LOCUS       DAEPOZ010000001        69992 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.001, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000001 DAEPOZ010000000
VERSION     DAEPOZ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 69992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69992
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.001"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..451)
                     /locus_tag="KR440_000001"
     CDS             complement(<1..451)
                     /locus_tag="KR440_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0697079.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLVFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLELYSKSSEIKAFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLI"
     gene            complement(448..1101)
                     /locus_tag="KR440_000002"
     CDS             complement(448..1101)
                     /locus_tag="KR440_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0697080.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYNDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            complement(1199..2575)
                     /locus_tag="KR440_000003"
     CDS             complement(1199..2575)
                     /locus_tag="KR440_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0697081.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKIFQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSSKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDVHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFVGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIARQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFIIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2575..3231)
                     /locus_tag="KR440_000004"
     CDS             complement(2575..3231)
                     /locus_tag="KR440_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0697082.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQKKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3241..4518)
                     /locus_tag="KR440_000005"
     CDS             complement(3241..4518)
                     /locus_tag="KR440_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0697083.1"
                     /translation="MIKNAIAYITRKRNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIGENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNKSENNKIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKHIIKIMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMLNITTLGQ
                     SYLILISIIVLSVVFASSLILIKKPKEILSKIS"
     gene            complement(4537..5427)
                     /locus_tag="KR440_000006"
     CDS             complement(4537..5427)
                     /locus_tag="KR440_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0697084.1"
                     /translation="MDRIKNIINGSTKKDKYKIRDRFRHLFQSVWFLITNSYFEGFKT
                     GKIYGGNLKKICVPGMNCYSCPGAKGSCPIGSLQAVIGNSKYKISYYIVGILFFIGAL
                     VGRFVCGWLCPFGLIQDLLHKIPFFKKVETFKFDKHLRKLKYIILLVFVIILPLFLVD
                     ILGQGSPYFCKLICPIGMLEGGLPLVLLNKSMRNAIGFLYYWKGIILIVTLLLSIIIY
                     RPFCKYICPLGAIYSIFNPISIFRYRLDKDKCINCGKCKKVCQMNIDPVENCNHLECI
                     RCGRCKNACPVDAISYGVRK"
     gene            complement(5594..6751)
                     /locus_tag="KR440_000007"
     CDS             complement(5594..6751)
                     /locus_tag="KR440_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBH0697085.1"
                     /translation="MKKIVSRGACLLLTLCLSLSTVGCKKSNESNKIKEGQSISSEEA
                     GMSTNEKDKNETFKPSDYTLKTKKEYVYEYLGLKFKLSDKFKKYMDDKKIAMLDDQSP
                     IDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELERIGTIGIFEKNTSEEKISK
                     ITKCDTHTKIGVSSDGKYDCYLSTNSGAESNLLDELKRTEIQIIDKKERPKNGFVLSE
                     KTDLENTEAFNKESVKDLRKLSTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEI
                     PDLVEVQNEMKSKGVNIVGVVTDTVDDNGENKEAIEKSKLIHEKTKASYPFLMPDKTN
                     FNGRLNGIQAMPETFFVDSNGNIVGDTYSGAKSSKEWKQVIEKELEKIKNK"
     gene            7085..7435
                     /gene="mobC"
                     /locus_tag="KR440_000008"
     CDS             7085..7435
                     /gene="mobC"
                     /locus_tag="KR440_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBH0697086.1"
                     /translation="MANRIRNERLEIKLTEEEKALFEEKRKLARCRNMSHFIRKCVLE
                     KEIYQVDLEPFRDLQGLLSNATSNINQIAKRVNSTGIIYKDDINDMKKQIEYFSKELW
                     QIHSLLLNRTSGGD"
     gene            7441..8772
                     /locus_tag="KR440_000009"
     CDS             7441..8772
                     /locus_tag="KR440_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBH0697087.1"
                     /translation="MAITKIHPIKSTLNLAISYIVNGKKTDEQILVSTHKCHQETAHT
                     QFLRTRNDVGTNGTVLARHLIQSFLPGEASPKMAHQIGLELCKKILKDEYEFVLSTHI
                     DKGHIHNHIIFNNVNMVTGKCYQSNKKSYHKIRYQSDKLCKENNLSVIDEFYESYKRK
                     YKTNGKSWYENEQSKRGTSWKSRLQFDIDRLIKQSKYWEEFLKKMAELGYEIKYGKHI
                     AFKPKDKTRFTRAKTIGEDYTEERLKERITENQSITTPPVKRRIGNIINMNANAKVKE
                     SKGYEYWATKHNLNTMAESVIFIREHGINSIKQLDKYIQKSAEERQDLQDKMKEIDKD
                     MQLLSDTMEQVHTVKKYRAYYKEYKANPSDKAFFEEHKAEITHYEIALSKLKKSYSKL
                     PNSKDILDKLNKLQEKKNTLIQEYSSTKSTMDELYQIRKNYGIYMGKEMER"
     gene            complement(8808..9614)
                     /locus_tag="KR440_000010"
     CDS             complement(8808..9614)
                     /locus_tag="KR440_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009354872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HBH0697088.1"
                     /translation="MEISKKELRKISRKFRILASNVMNSYYGEQNANLEEMINYIEQT
                     LLIKEYLHSLEYDVDGLDDLLNTISNSYGRKVLNLGNDSNKRTYLLYRAFSFITGNNL
                     PTYTFGWYYAGSNKYQDMAKAFGDRMVYPFVLEVENYMKDISTDMGYDENNALYNINV
                     NSSGVQVNIAEHGSTINADQENKFNTEEVIKAINNVEDLIKGMEDDNSKSILNQNLDN
                     IKNEIKQKHPKKEVLTTCLKSMHFITTSVALIPDLALGIKMLASLIGISI"
     gene            complement(9604..10065)
                     /locus_tag="KR440_000011"
     CDS             complement(9604..10065)
                     /locus_tag="KR440_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBH0697089.1"
                     /translation="MANDIKFEISVPADEEGYVQLKCPSCSEKFMLTVEDIEAESTID
                     IWCPNCGLTHENYLDDDIYELAERIVENQVAEMLNDFSKNLEKSFRNSKSIKFKRGKE
                     IKKEAELPIGRKTGDYEEKKYLCCGKTARISSIKKLEGSYCPFCGEMIDGD"
     gene            complement(10093..11160)
                     /locus_tag="KR440_000012"
     CDS             complement(10093..11160)
                     /locus_tag="KR440_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009354804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HBH0697090.1"
                     /translation="MSGKLYIAAAGSGKTRKIIEDSLNTHKKILILTYTITNEQQIIS
                     RIKNKVGVIPNNITVSTWFSFLLKDGIRPYQGSRFKERIEGIIFVNGQNNTYVKKTSN
                     KYFAKDNRVYTDKLSECVVELNDKCNGKVFERISGMYDCIYVDEVQDMVGYDLEILKC
                     LIASRIEVIMVGDPRQCTYSTHSSKKYKKYSCGKIDEFLKNECRKLDVRIDETTLNCT
                     YRNNDIICKFANSLYPERTSTNFVRRAEDESDGVMIIDKADVEKYLQKFNAMQLRENK
                     RTSVNNEYPYYNFGESKGLEFQRVLIYPTQPMWDWIINNNTSLKEQSKARLYVAVTRA
                     ENSVVIVRKDARPCSLPVVKL"
     gene            complement(11157..12779)
                     /locus_tag="KR440_000013"
     CDS             complement(11157..12779)
                     /locus_tag="KR440_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0697091.1"
                     /translation="MSIRKLKIKNFKCFSDWFIVDFENGINILVGNNGTGKSTILEAI
                     NLVLTGTYHGKNIRNELTQYLFNKNIVENYIREVNEGVNVEPPKIIIEAYFDAEMPSF
                     LGDGNSEKTKDTPGICLSISFDEKYSNEYKMLCKNKITSLPIEYYEVSWMSFARENIT
                     ARSIPIKSAMIDTTSYTPTSGSDIYISRIVKNLLDDNEIINISQAHRLMKEQFSRDEN
                     IANINRKLTETSNNELTISVDFGTKTSWESSLVTQLNEIPYNNIGKGTQCIVKTQLAL
                     SDHKIENKQIVLLEEPECHLSYSNLNILLDKVNESVKEKQIIVTTHSSFVSNKLGLEN
                     LILLNRGKTLRLGDLSNETNLFFKRIAGYDTLRLLLSNKAILVEGDSDELIVQRAYMD
                     SHNGKLPINDGIDIISVGISFLRFLEIAKLLELRVSVITDNDGNIDALNRKYSDYLGD
                     NCCENINIYFDDSTHEYNGSLENYNYNTLEPCIYRSNSMSILNEILNKSYEDPDDLLK
                     YMKGHKTEVALKIFESDKAIEFPQYIKEGILI"
     gene            complement(12982..13212)
                     /locus_tag="KR440_000014"
     CDS             complement(12982..13212)
                     /locus_tag="KR440_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0697092.1"
                     /translation="MRVFSYKRLFKKLIDLDMTNNELMEKAKVSKSTFYKMKNGQNVT
                     TDVLLRICNALDCDIEDIMECVIIEDVRRMGV"
     gene            complement(13352..14011)
                     /locus_tag="KR440_000015"
     CDS             complement(13352..14011)
                     /locus_tag="KR440_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006627214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBH0697093.1"
                     /translation="MDYKTMRDRVEDMLSDNHKDFVKAIISMEKGINDESALDKLYDA
                     YMDNDSLNLLHEEFDYMIEDLRGQGQIKDLLYVREEKDNLINIVGNIVGEVDVIEREN
                     KNGETFKVANFSVVSKDDEGNKLYHNCSVYGEKSDIPKDFKQGDFVKLFGQIRTSIDD
                     NGKEHSNIRILSSKLLKAKEQMKGQEEKKESILGAIKKYQAEDKEKPKEKKEASKEAE
                     R"
     gene            complement(14054..22783)
                     /locus_tag="KR440_000016"
     CDS             complement(14054..22783)
                     /locus_tag="KR440_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBH0697094.1"
                     /translation="MRINDFHNILELIKQDVLYNEAEYLKLLKVVGNNQRYDFRSQLS
                     IYDKNPEATACAKFDYWRERFNRTVMRGQKGIPILEDYGTYKKVDYIFDISQTVSKNR
                     DVNEVNLWRFDKEAHQDVLKEMIKSEGYEESESTLENIFSLSRLYGDEKIDTLMNELR
                     VADEDRISFTKFVRDSVSYAVASRFKLDYPMDYELLRENFQRFDSISLMSLGESVSDI
                     SGKIIDETIQKSKELELQKEVLIGKEAGYNKIKEELEEVEENVFRRDDQERNENERVL
                     RNGEYGRDNRKNQGEYAKQLGGTDGLYKEVSESDLRSDEAELPFTERGAEPLRDAFGS
                     IQGEEADRTPDGYSETGSRVYESREAKVDSSLEDRGREQSAVWGDDFSSQRNDHQGSS
                     GDLKDNTDKGIIEAEKASFSLPENSYGQISLTIPLTEKDIDTVLINGGNHDGGRLPVI
                     AEFSKGKSIEELGEYLKDTFKGGNGFYIDEREVSSWYSDKGIHLAYGTSAREDNTQIL
                     SWSDAASRINELLDRGEFATNVELLEAQDYERDRISESLWYLTHDLSEEGIEQGYFNS
                     FERGGGFPQETKRLSEALKNPEYLKETIKEYSRFLEGYKESRNVLRYHYHKVDSLYQK
                     LQELELQRKEYSTNLTELPKVKLFITEDEVLESLSRGSGVDRGKERITKFFKENHTLQ
                     EKANFLKDEYGIGGHSHVVSGAMGSDEWHDAKGFKLQKNDCNDVFLTWTSVAKHIDEL
                     LSKKLYFEEKETESTAEIEEVKEPQYYSKNDPGNLMPDEILERVPEIYAQEDVDLADK
                     QVHAAYIIPFRSNWTWYMTEYDRESGDAFGLVLGIEPEWGYFNLEELKELNAQRLILE
                     DFPKTFRELKDIELIKQMDEQELQFVFNGELSFEDKVELEAPEETEEATKRGTVQATL
                     FDYLKEREEVELNEKEDSLLEEFAVKAGDTVYFNHEEYKVREISKNEITGRYDLWLDP
                     VRSGNHQIPIVDFTDNEDLLNKISLERPNFIVGDEVKYKDKNHTITRFDDMRKNLNTV
                     TVKDNTEYFGGMITSFDVIPYRLESDLERVFENLTYKQPEQTTEETQIRKAEAHNFKI
                     TEETLPEKLSPSERLNNNLEAISMLNRVESGQRELDSTAQEVLARYVGWGGLSEVFDE
                     SKEGQWKEARAFLNENLSHAEYEAARESTLTSFYTPKTVIDGIYKTLSDMGFKQGNIL
                     EPSMGIGNFIGNIPDEMNKSKFYGIELDSVSGRIGKLLYPESEVQIKGLEETSFSNNF
                     FDAVIGNIPFGEYKVNDREYNKNNFLIHDYFFAKSIDKVRNGGIIAFITSSGTMDKKD
                     ESVRRYLAARAEFLGAIRLPNDTFKGIAGTEVTSDIIFLKKRDSIRERDEDWIHLAED
                     ENGLTYNKYFVDHPEQVLGTMREVSGRFGNTLACLPKENADLKELLTKASEEISKGST
                     YEEIELLDDEITSIPATDDVKNFSYTVIDDEVYYRENSLFIKKEITDKNKEKIKDYLE
                     LNTALKDVIYKQKEDYSDDEVKKAQEKLNEVYDNFSKKHGFINNLSNTRALKEDSNFP
                     LVSSIEILDEEENVKAKGDIFSKRTITKAKVIDHVDTSLEALVLSVSEKGYVDFEYME
                     SLTGKDRPTLIEELRGEIYLNIREEQNFYRPLSFNLEDGDLPFACANGSNSYKYGYVT
                     KDEYLSGNIRDKIAIVDSYLAKLRQTERELPHLGFVENGKEKELISYEMNRLEYQKAE
                     LTKVLPKELEASEINVRLGATWIPIKDIEKFIFETLKTPGYARWDIKVKFSNITSEWN
                     VEGKSRDKGNDLAEMTFGTSRVNAYKLIEDALNLKETKVFDQIINPDGSKTSVLNKKE
                     TMLAGQKQELLKEEFKNWIFNDQERRNRLVKLYNERFNSIRNREYDGSNLSFEGMNTE
                     IDLRPHQRNAIARSLYGGNTLLAHVVGSGKTFEMVASAMESKRLGMCSKSLFVVPNHL
                     TGQIGREFMQLYPSANIMVADKKDFEPKNRKRFIGKIATGEYDAVVIGHTQFEKIPMS
                     KEYQEKHIQDQINEIVNYVEEYKHDRNQNFTVKQLEKTKKKLETRLEKLNDDFKKDDV
                     ITFEELGVDMLFVDEAHNYKNLYLYTKMRNVAGIGQSEAFKSSDMFMKCRYMDEMTGG
                     KGIVFATGTPVSNSMTELYTMQRYLQYESLKKNHLEHFDSWASTFGETQSSFELSPEG
                     TGYRVKTRFSKFYNLPELMSMFKEVADIQTADMLNLPTPEANYEVIKTEPSEEQKEIL
                     KSLSERADDVRNRVVEPDVDNMLKITNDGKKLALDQRLINPLLPDNPDSKVNVCVKNV
                     FAIWDKTKENRSTQLLFSDMSTPKGDGEFNIYDDIREKLVAMGIPKEEIAFIHEANSD
                     KQKDELFAKVRKGEVRILMGSTQKMGAGTNVQNKLIALHDLDVPWRPADLEQRAGRIV
                     RQGNENEKVNIYRYVTENTFDAYLWQTIENKQKFISQIMTSKTPVRVAEDVDESSLNY
                     AEIKALATGDPKIKEKMDLDNEVTKLKMLEANYKSNRYRLEDKVAKNYPEEIARTEKL
                     IEAVKKDISNVEPKAEGEEKFTSITIAGEKITDKKLAGERLLEAISKVKINESKVIGK
                     YRNMDLEVSYNFFTNSHNFSLNGVAKHLGELGTSADGNITRLDNALEKMPEKLNMLEE
                     KLISTKEQLENAKEELKKPFEKADELKTKVLRLAELNKLLDMGEVEEKKNDNPLVEDV
                     KRAIIDFCNREYEENHSYDEFHTLYPDLKHIGIAYTNTPDERHGIQYELNLEEKTWTQ
                     YIDDTPIKTESFDYENKGENEALRNMKNEIELSTFSDLVYVDSEDLRVATGLDIDDEG
                     NFYDPFAKDLDNDGIPDRYDSDFKDSDYFESTYDVEDNLHSKEETTQKSDDKPSILGQ
                     IRAYQEESKTEEKQKIKEQEFLR"
     gene            complement(22866..23669)
                     /locus_tag="KR440_000017"
     CDS             complement(22866..23669)
                     /locus_tag="KR440_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004804477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HBH0697095.1"
                     /translation="MSKINVEGSEISIIAIDNRDYISLTDMVRNIENGSALIEKWLRN
                     KNTIEFLGIWEEMYNTNFNSPEFEGIKNEAGFNRFILSVKQWVEKTNSIGIVARAGRY
                     GGTYAHKDIAFEFASWVSPYFKLYLIKEFERLKEEEQKKLGWDIKRNLAKINYRIHTD
                     AIKNKLVPDKLSKEKINFIYASEADILNVSLFGITAKEWRDENPDLKGNIRDYADISQ
                     LVCLSNLENLNAVFINEGMSQSERLEKLNAIAIEQMKILSESESIKKLK"
     gene            complement(23754..25460)
                     /locus_tag="KR440_000018"
     CDS             complement(23754..25460)
                     /locus_tag="KR440_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBH0697096.1"
                     /translation="MKLVIAEKPSVAISIAKVIGANKKKDGYYEGNGYRVSWCVGHLI
                     QMANPDAYDEKYAKWNMADLPIIPSDYKYEVAKATKKQFNILKKLMNDKEVDTVINAC
                     DAGREGESIFRLVYNQVNCKKKMKRLWISSMEDSAIKEGFDNLTDGKDYDNLFESAQA
                     RAIADWLVGMNISRLYSCLYQQNYSVGRVQTPTLAMIVKRDDEIANYQKEKYYTVELS
                     MNGFTLSTDRIDDEVAAEQLISLVGDKIEITDVIQKEKITKPDLPFDLTTLQRECNKY
                     FGYSAKQTLDYTQSLYEKKLITYPRTDSRCLTEDMIVSTVNNILGKNDFDTERIKVVF
                     NSKKVTDHHAIIPTISSVKEDVSELPLSEAKVYFLISDKFHASVGYPLIENTTKIVAS
                     FDGFEFTSSGKVIKDEGFSKYLKEYKSKKSEDAVLPDVSVGDVLSVENKEVKEKFTQP
                     PKHFTEDTLLKSMEIAGNDALEKGVEVERKGLGTPATRAGIIENLIFKRFVERDKKNL
                     IATHKGISLVTIVADTFKSAETTAKWEMELSDIAQGKSSKKEFLDAIENEIKEAVLTY
                     RK"
     gene            complement(25555..27096)
                     /locus_tag="KR440_000019"
     CDS             complement(25555..27096)
                     /locus_tag="KR440_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HBH0697097.1"
                     /translation="MKTSLKKNNKFWTVALAVIVSISCILGLWTVVYAQEQKSEAPTK
                     NEAVQTEVEVNVRYIFEDEKVFKEEKIKAEKGQLIDSGDLPMLPDDMKFIDEFLFYEV
                     KGDGKDEIIRKVAKMEVKDKETQTEEEKPKQDECTQTEDKGTQTELSKDDISKIEKEA
                     KELQDKLDKLNCELKDKDKLSDKQKNKIKDLEDEIDSLKEKMKKDKGNKDLSEDMKKE
                     IDKLTEKVKELEKKATETNKAPVTSQSVTPISPISGIKTSSGISPQTPQTSVSSTGKG
                     SSDSVSSGNTTKDTGKGKTEVKEKEKEVRYPNKLTAKAPANNSQDSSMGSASKSVNTN
                     KGVASSPSKARASVTENKDNANKEYPIHHGDSSDNKETDQYSADARQFITFQTKNGKT
                     FHLIINHDEDSENVMLLTEVSEDDLLNMVEKKEAPKQEVVKEEPVKEEVKPEKKDEKS
                     NLGTYIILLLVVGGALGAGYYFKVVKKKEDKELEALEEEDDDFFSESESEEEINDADE
                     VEDEE"
     gene            complement(27071..27310)
                     /locus_tag="KR440_000020"
     CDS             complement(27071..27310)
                     /locus_tag="KR440_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH0697098.1"
                     /translation="MKKELTTVKNRIKKLKDKKALIDEELEPLFIREEELENEEIIAI
                     CRKNNITISDLIAKVNRQKAEMKKEKTNENQFEKE"
     gene            complement(27322..29454)
                     /locus_tag="KR440_000021"
     CDS             complement(27322..29454)
                     /locus_tag="KR440_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBH0697099.1"
                     /translation="MGKKLKKDFKERHKASLEREMFHSKTDTIPEESKLKHNDDYRGK
                     IVHEKERFQDKVHEKTSKVSADNEKSYGTSKRNEKYRTSDKGDVNSAIETGRSDYITE
                     VKDGKIYDPLGKDLDNDGIIDRYDNDFRDSNYFESTYDVEDNLSHKEKSLENSFKNHK
                     TQKSRYKRKNYSESLYTRKKDDAPKGNKADDKKAGKDAVSGKVYSSLSKDQKKKLKKD
                     MVKVSALSGLAKGSETVRDYLSHGSDENQGVEAGEKTADTSSKLIHGIKKYSDKKRAK
                     RGYDLTNKDYKIRKRKSKLEFRDAKKELKKTDEYKRASAYKRFQKKNQVKAAISRENK
                     SRLRDRIKEGLIGTLKSSKDMIIRKAKGLMLIFIGIIILGTFVINFAGTGMTGFMNST
                     SSVLTTSYLSKPNVLSEINQKFSDMEGELQSEVDYVKENYPGYDEYILNNTEYIGHNV
                     HELLSYITSRCGEVKSVSEVESILKELFESMYDLEYREEIEIRYRTVTETYTDEDGNE
                     YTESHEEPYEYKKLIVTLHKKEMDSIIRKVFANYPDNLKHYEALFLAQGNMREAFGNS
                     DLISANGGIGGGKEYEASTEVQKKIVNAAYITPSPGAGWCAMWVSQVYQNAGLGYIGG
                     NACDMYRNYTFTSDRSKLKVGMLVAVESSSSGSSAGLTYGHVGIYIGDGKVIDNIGRI
                     RVTTLDDWIATFCKHHPVGFGFPPNVKK"
     gene            complement(29458..31887)
                     /locus_tag="KR440_000022"
     CDS             complement(29458..31887)
                     /locus_tag="KR440_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0697100.1"
                     /translation="MNKKRKIQQIKLMQNEKALRSQKRDLKKSNCKSKKDKGGILDLI
                     FKKEPKRYTVEDTIPYLRLLKSGICQLDEKHFSKSIAFQDINYQLALDEDRDLIFNQF
                     ANFLNSFDPSISIEFSYINQLGRNEEMKSAIQIPDKKDGFDDIRFEFREMLKSQIVKG
                     NNGLKKSKYVTFTVEADNLEQATSKLERLEIDILSSLKSMGVRAESLNGEERLKILHD
                     VLNPNKTFKFSYKDLKKRESTKTYIVPDEFNFTPSRYFKFGKFIGATSHFQILASELS
                     DRMLSEFLDIDDNINISFHIRAIDQSEAIKMVKRKNTDIDKMKIEENKKAVRSGYDMD
                     ILPSDLITYGEDVKSLLKDLQTRDERMFVVSIVFMNFARTVQKLDNTIAQISSIANKH
                     NCKLKRLDHSQEQGLVSVLPLGVNKIEIDRGLTSSSTAVFMPFTTEELFINSSNSLYY
                     GLNALSHNLIMADRKKLKNPNGLILGTPGSGKSFSAKREMANAILVTDDDVIICDPEG
                     EYGNLVRQFKGEVIKVSSKSKDYLNPLDINMNYGDGDAPLKDKANFIMSMLELVVGGS
                     GLTAEEKSVIDRCLPKIYEKYFEHPTPDNMPILQDLYDMLKGQEEKVGKKLATEMEIY
                     VSGSLNVFNHRSNVDLNKKLLCFDIKELGSQLKKIGMLVIQDQVWNKVSQNRGNKATR
                     YYIDEFHLLLKDEQTASYSVEIWKRFRKWGGIPTGITQNVKDLLMSKEIENIFDNTDF
                     VLMLNQASGDREILARKLKISLPQLRYVTNSNEGEGLLFFGNTIVPFIDKFPKDTILY
                     QKMTTKPEEVR"
     gene            complement(31799..32188)
                     /locus_tag="KR440_000023"
     CDS             complement(31799..32188)
                     /locus_tag="KR440_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HBH0697101.1"
                     /translation="MAYVPIPKDLNRVKTKVAFNLTKRQLIGFTLAGLVGIPVYLFMR
                     KFVPNDIAVIFLIVSTLPIFFITLFEKDGLTFEKYFKYIYLHKFYQPQKRVRKEVYLE
                     QEKKNSANKTHAKRKGIEKSKAGLKEK"
     gene            complement(32192..32452)
                     /locus_tag="KR440_000024"
     CDS             complement(32192..32452)
                     /locus_tag="KR440_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH0697102.1"
                     /translation="MNKRKTVFIVVIGAGIMAIIDRIRLHNKVEELEEKTEDIGRCHN
                     DFYLIQQRYNKNTDEQLATIQEEIGSVYEHFEELSKGKEDGR"
     gene            complement(32470..33333)
                     /locus_tag="KR440_000025"
     CDS             complement(32470..33333)
                     /locus_tag="KR440_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697103.1"
                     /translation="MFGIFDKIEEFFKELLLGGIQANLESMFLDINDKVGAVATDVGK
                     TPMGWNGDVFAFIKSINDSVIIPIAGLIITAVLCIELINMVMQKNNMHDTDTFEFFKY
                     IIKMWIAVWLVSHAFEFSMAVFDVAQHMVNKAAGVINTSAVISGDQIVKMVDGLKDKG
                     LGELVMILFETSLIKVAIQVISIVIMLVVYGRMFEIYVYSSISAIPFATMGNKEWGQI
                     GTNYIKGLFAIGLQGLFLMVCLGIYAVLVKTIQITDIHTSTFTILGYAVLLGLMMLKS
                     GTLAKSVLNAH"
     gene            complement(33353..33568)
                     /locus_tag="KR440_000026"
     CDS             complement(33353..33568)
                     /locus_tag="KR440_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HBH0697104.1"
                     /translation="MEFFAQAVNVLKILVMAVGAGLGAWGVINLMEGYGNDNPGAKSQ
                     GIKQLMAGGGIVLIGLKLIPLLANVLK"
     gene            complement(33572..33883)
                     /locus_tag="KR440_000027"
     CDS             complement(33572..33883)
                     /locus_tag="KR440_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBH0697105.1"
                     /translation="MKQEMININANLVAEPTFSSFEKEGEEVEVVNFTLVKKYGKGKE
                     YINCAAYGEKAEKAEDFEKGDLIHVFGYFKKREKEGKTYKNFVVKSYNKIEKKEENEE
                     E"
     gene            complement(34047..34280)
                     /locus_tag="KR440_000028"
                     /pseudo
     CDS             complement(34047..34280)
                     /locus_tag="KR440_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
     gene            34422..34595
                     /locus_tag="KR440_000029"
     CDS             34422..34595
                     /locus_tag="KR440_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0697106.1"
                     /translation="MDYMTLKEASEKWGVSSRQINYYCTDGRIPGAVKMAGVWLIPKE
                     AEKPKDRRYNESR"
     gene            34600..35310
                     /locus_tag="KR440_000030"
     CDS             34600..35310
                     /locus_tag="KR440_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0697107.1"
                     /translation="MLHIAICDDEKDFVQYLTDLLNQYSKETGRDIKITPYYDGMELI
                     EKYDPTIDLIFLDIQMKVVDGLHAAERIRQMDETVGIIFLTTLTQYGLEGYKYHATNY
                     IIKPMKYARLKSEMDKFLERSQKEDTPSLVITNDTGKYKVPLKSIRYVETYNRNLMFH
                     TEQENIICYKSMKEVERELCDKDFVRCHTSYIVNLFYVKGIKKLDIELITGEIIPISQ
                     PKRKEFMERLTDYWGDML"
     gene            complement(35581..35742)
                     /locus_tag="KR440_000031"
                     /pseudo
     CDS             complement(35581..35742)
                     /locus_tag="KR440_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569186.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            36353..36538
                     /locus_tag="KR440_000032"
     CDS             36353..36538
                     /locus_tag="KR440_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH0697108.1"
                     /translation="MLKKYWIKCPICNGKTRVQVFHNTVLKDFPLFCPKCKLTHIIDV
                     EKLEIVIKNTEKQTFII"
     gene            36555..37538
                     /locus_tag="KR440_000033"
     CDS             36555..37538
                     /locus_tag="KR440_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBH0697109.1"
                     /translation="MKRLPKYTPAEVRNDPYGFTYKEMSEVIGENEAKALYEELYKQL
                     PRKKNLSMLVKNICKSSDTEKYVYELKDNKYIETVFIKRRDGGTVCVSTQVGCPVGCI
                     FCESGRNGFVRNLTSSEIVQQIILLRRKVNRIVFMGMGEPLFNYDNLIKAIHILRDRY
                     GLNFPTDGITISTVGPVDQLKKLREEHLKIQLTISLHAATQSARNRIIPHMRIYAIED
                     VVKQALSYSERHNRKIVFAYLLLPGINDRPSDVRQLAKWFRGKKVMINVLQYNPTSNS
                     RIKAPQKREIVAFKHQLEQAGLEVTMRVSHGREINAACGQLANTYNKFKKK"
     gene            38012..38449
                     /locus_tag="KR440_000034"
     CDS             38012..38449
                     /locus_tag="KR440_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003508282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH0697110.1"
                     /translation="MELSSSDKERIQHQYDALAKKTLVGEAKSHRRTLAKRAAREVTF
                     SDLSESELAQLFTTDEYESDYFRFQVSGFDVLVKNELLAEALNALPERKRDIILLSYF
                     LDMSDAEIGELLNVVRTTVFRHRKSALAKIKQYLEGKADDEYR"
     gene            38433..38675
                     /locus_tag="KR440_000035"
     CDS             38433..38675
                     /locus_tag="KR440_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0697111.1"
                     /translation="MMNTVRKSENLLPFPVISAAANGDTTAMCAILKHYEGYIAKLCT
                     RTLKDDAGNTYSYVDEEMRNRLQVRLITRTLAFHVG"
     gene            39134..39307
                     /locus_tag="KR440_000036"
     CDS             39134..39307
                     /locus_tag="KR440_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-encoded TnpW family protein"
                     /protein_id="HBH0697112.1"
                     /translation="MDKKQTITTERKIGKITYLVQALPSEKATDTIHKKIEKLIVKDL
                     QKKPGNPGFLASE"
     gene            39385..41058
                     /locus_tag="KR440_000037"
     CDS             39385..41058
                     /locus_tag="KR440_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH0697113.1"
                     /translation="MLQSNKITALYCRLSQEDMQAGESGSIQHQKMILQRYADEHHFL
                     NTKFFVDDGFSGVSFEREGLQAMLQEVEAGRVATVITKDLSRLGRNYLKTGELIEIVF
                     PENGVRYIAINDGVDTAREDNEFTPLRNWFNEFYARDTSKKIRAVKQAQAQKGERVNG
                     EYPYGYIPDPNNRHHLIPDPETAPIVKQVFAMFVSGVRMCEIQKWLAENKVLTIGALR
                     YQRTGQARYQRAMIAPYTWPDKTLYDILARQEYLGHTITAKTHKVSYKSKKTRKNEEE
                     QRYFFPNTHEPLVDEETFELAQKRIATRHRPTKAAEIDIFSGLLFCAGCRHKMYYQQG
                     VNIEPRKFSYSCGAWRNRARTGSECTSHYIRKNVLLDLVLEDMRRVLRYVKEHEQDFI
                     CKATEYGDMEARKALAQQQKELFKAQARMTELDTLFRKLYEDNALGRLTDERFVFLTS
                     GYVDEKKSLAARIDELQQQIATVTERKRDISRFIQIVGKYSDIQELTYENVHEFIDRI
                     LIHELDRETNTRKIEIHYSFVGQVDTEQEPTQVVNHDRRNMVDVKSIAI"
     gene            41082..41537
                     /locus_tag="KR440_000038"
     CDS             41082..41537
                     /locus_tag="KR440_000038"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697114.1"
                     /translation="MKEYTLIIKGEMDFIILSPQVLSSLITQIHNSPERKVVVSIESI
                     MPPKFTDYLLRVINSNRFSNERFRYRYILENPVTKKGMYEILRQQLSRTNTERFPCFQ
                     TIQLTDTFQGNVELDMECNDLFFWACKDTAAKFVYTFPDGREETLVIEY"
     gene            complement(41585..43237)
                     /locus_tag="KR440_000039"
     CDS             complement(41585..43237)
                     /locus_tag="KR440_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008978103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH0697115.1"
                     /translation="MSRKKQVYEKITALYCRISLDDGGDNESMSISNQKLMLRDFAEK
                     HGMFQYEYYVDDGYTGRNFNRPSFQRMIADIEAGKIGCVITKDLSRLGRNYIEAGSYI
                     EIFFPKHNVRYIAVTDGVDSLTRQEMDITPFKNILNDMYSRDISKKVLAGRMTRSRQG
                     KFCGGQPPLGLMRDPEDKGHLILDPETAPVIRKIYDMALDGWGCMRIAKQLMDDKIPI
                     TRVKSNTECDVNYYSWGSARISHILRNPFYKGAHLVCRTHQKGIRSNTYDIIPREDWE
                     VIEGCHEAIVTPEEWEQVQDIIDRRPTIMKGNACPFYNLFHGLVYCATCGKSMQVRYE
                     KVGRTGKNRFTGEMREPIDRAYYICQTYNRLGKNACTSHKIEARDLYNLVLKDIQELA
                     AQAMKDADAFYQRLSSRMERRYLVDASQTEKERKRLEDRNQEIDGMFLSLYTDKAKGV
                     LTEQRFMKLTAALEQEQEANQKRLHDLAVMQSRADAQESEVRTFIKEIRRYAAIAELD
                     ESVLNRLISKILIGEVKKVDGQKVQEVRIVYNFVGEIPEIAA"
     gene            complement(43310..43477)
                     /locus_tag="KR440_000040"
     CDS             complement(43310..43477)
                     /locus_tag="KR440_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008978104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697116.1"
                     /translation="MEKSKNQKNKVRFVVVREFSGEKSMREAFEQLIERQTCEHFEEW
                     RHQREMAEKAA"
     gene            complement(43745..45103)
                     /locus_tag="KR440_000041"
     CDS             complement(43745..45103)
                     /locus_tag="KR440_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated protein E"
                     /protein_id="HBH0697117.1"
                     /translation="MSAAEALAPEQSVDEVREGLSVTEKGQPANTIGNCRTVFSHDPL
                     LRDAIRLNLLTDRVDIVRDLGWRRNTSALTDTDVKYLLLYFEQNYGLTSEKKMTAALS
                     IVANENCYHPIQDVLNGLVWDGTPRIRSCLHHFLGAEVSDYVEEMLKHFLLGAIRRVF
                     SPGSKYEEMLCLVGGQGAGKSSFFRLLAIRDEWFSDDLKKLDDDRVFLKLQGHWIIEM
                     SEMLATSSAKSIEEIRSFISRQKETYRTPYEAQPKDRLRQCVFGGSSNTLDFLPLDRA
                     GNRRFLPIMIYPEKAEVHILEDEDASRAYLLQVWAEAMTIYRSGKYSMKFSKSIQRQL
                     VEVQKDFMPEDTEAGQIQGFLEHYTGSMVCSKQLFKEALGHTYDEPKRWQLHNINEIM
                     NTVVTGWKPFSNPRMFAGYGRQKGWERDVSGNELPGNEDGFVELSEEECRQLELPKEW
                     IA"
     gene            complement(45063..45749)
                     /locus_tag="KR440_000042"
     CDS             complement(45063..45749)
                     /locus_tag="KR440_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBH0697118.1"
                     /translation="MKTGCLFLCLEVRKSNVFEAVKQAVPTRQAAESYGIQLGRNGMT
                     CCPFHDDKHPSMKVDRRFHCFGCQADGDVIDFTARLFGLNKKDAALKLAEDFSVGFDA
                     KGHDPPRRKPGKRKISEELRYRQAEQKCFRVLCDYLHLLERWEKEYAPQTPEEAWNPL
                     FVEALQKKPYTEYLLDILLSGSMEERACVVAEYGKEVRKIEQRISEFTASHPAGRHER
                     SRSFSAGAER"
     gene            complement(45765..47009)
                     /locus_tag="KR440_000043"
     CDS             complement(45765..47009)
                     /locus_tag="KR440_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/arginine repetitive matrix protein 2"
                     /protein_id="HBH0697119.1"
                     /translation="MKLTRHNGRSGKHGTYNPRHNDRRFDVENSEHIDAERARQNVYW
                     DCYRGFTTHEFRENPEQPDFSFEEIERMYYNEHYGGHVEAQNARNEKTRHTERNRTVE
                     DLLKNNKTCPEESIYQIGTMGESVPPDTLFSIVNEFYEEFERRFGSHIHILDWALHLD
                     EGTPHIHERHVFDCENRYGELCPQQEKALEELGIPLPNPEKPKGRNNNRKQTFDAVCR
                     TILFDIARRHGLHLDQEPSYGGRDYLEKQDYILMKQKEQLAAQEQKLEELTLKIEDVE
                     TLLDDVSDAAYDKAVEVVTDTVRQETHKEDIRLVEESKKWVLSPERKAPKKEREYAAE
                     RLDGVITKIKNAMQHALAKIQRTLMQPEVKQAGKEQVKKKAKESIMDILAKAKINADR
                     DNRERWEREGRIAPTKKNDIEL"
     gene            complement(47437..47742)
                     /locus_tag="KR440_000044"
     CDS             complement(47437..47742)
                     /locus_tag="KR440_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBH0697120.1"
                     /translation="MRKLSKYEKETIINWNEAENLASVYTFNASLKRRLADFSRKYPL
                     LCRLERSTPEGSVTYVLDKSRLSIRLVPPYSEERKAAASAYAKEHGFQVVGAEEKIA"
     gene            complement(47726..48019)
                     /locus_tag="KR440_000045"
     CDS             complement(47726..48019)
                     /locus_tag="KR440_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697121.1"
                     /translation="MTLSMKEKKILYAYGCPSHHNTVTRLKWLTALTVDPEAKRRMLG
                     LARKVETEVGENWYEDFYHHLRMEMDEYRRLKRSLRVLKSYTDYEEDLYEEAV"
     gene            48385..49233
                     /locus_tag="KR440_000046"
     CDS             48385..49233
                     /locus_tag="KR440_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0697122.1"
                     /translation="MYAKLTIPERLKDLRVVDKHLTLEQLAEQTGLSKSALGKYETDD
                     YKDISPFAIATLADFYGVSTDYLMGLTENKNHPNIELQSLHLSDDMVELLSSGKINNR
                     LLCELATHPNFRRLMIDMEICIDQIANMRVEQMNLVLEATRQTVMNKYAPGEDDLYMR
                     TLELGQVQESDFYSHVMHDDLDSIVRDIREAHRKDKTTADPQPTLDDVKEKFEQAIGQ
                     GSDTEMLIHEFCDKLQIPFEKISSEDFSAFLRILSLSKQLKSSNNMRGKASPIPPNRK
                     ARRKRK"
     gene            complement(49355..49636)
                     /locus_tag="KR440_000047"
     CDS             complement(49355..49636)
                     /locus_tag="KR440_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697123.1"
                     /translation="MILEEMYNGRFYPCETVVADSPRFKQAVKASAALMDTLSERLSK
                     EDYALVEELREQVAIAQCEENESHFKYGFSAGLLVQQEAHAQVSQKDKE"
     gene            complement(49650..50027)
                     /locus_tag="KR440_000048"
     CDS             complement(49650..50027)
                     /locus_tag="KR440_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HBH0697124.1"
                     /translation="MEHLPKKIHHNGISYTLVGDYYIPDLKLSEEIRPIGRWGRIHKA
                     FLQEHRPGQYNELLLSGKLWTYLADLNEQADDRLACIISQMQAAEGVTEELKARDQLA
                     WVGGMNSIRSRVEEIILSEMIFV"
     gene            complement(50125..51687)
                     /locus_tag="KR440_000049"
                     /pseudo
     CDS             complement(50125..51687)
                     /locus_tag="KR440_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
     gene            complement(51684..52169)
                     /locus_tag="KR440_000050"
     CDS             complement(51684..52169)
                     /locus_tag="KR440_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HBH0697125.1"
                     /translation="MINEEIARKTLNMEINAGKVTGKVLLNLLKKLMKEAEKFGGLEK
                     LVNANGNEVKLKDMVKKGQLEEIPVEEAELKELKKELNRYGVKFSVMKNKETGKNSVF
                     FQAKDMKVMDKAFKHALSKSEKKAERKESIHKNIEKFKEMAKNSISKEKIKNKQKEQS
                     L"
     gene            complement(52171..53193)
                     /locus_tag="KR440_000051"
     CDS             complement(52171..53193)
                     /locus_tag="KR440_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH0697126.1"
                     /translation="MYLFDEQPIVANKTLAREIGLNEALILQQINYWIEINKKSGKNY
                     HDGKYWTYNSIRAWQENDFDYMSFDTVKRTFSKLEKAGYLLVGNYNKDPRDKTKWYTI
                     NNDKLEELYMDMDLKKQKIQTEALKNSMPNALGQNAPMDKGKISQCNNADCTNAQVQN
                     ALMQKDNLHQPLPENTTNNTTNNTTNNSAYISIHPSIKEKNQLFSENMKGGRIDISYK
                     NIYQNCLEELRHQTGYYEHMSIGNKSVAEEFDEILKILADVMVLDDESFITINQTKIS
                     VQVVKERFRKLNSGHLEYLVGVLRENEYRIRNIRAFVLTAAYNAPSGIDAYYSALVSY
                     DMRGGY"
     gene            complement(53279..53560)
                     /locus_tag="KR440_000052"
     CDS             complement(53279..53560)
                     /locus_tag="KR440_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697127.1"
                     /translation="MRWILKIILFPISIALSILIAFLSFLLGIGTAILYLLMMFCIFG
                     AIASFLQKEVTIGIEALILGFLLSPYGIPMVGTAVIAVLTGVKEAIRSI"
     gene            complement(53579..55213)
                     /gene="rlmD"
                     /locus_tag="KR440_000053"
     CDS             complement(53579..55213)
                     /gene="rlmD"
                     /locus_tag="KR440_000053"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBH0697128.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAEGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVETVALLS
                     KLDVDKHIDVEIKLDELDLTSAESKATYAQIKEYVLEKFGLKVSTLYIAQIKKKCGIE
                     LREHYNKSKKDNQAIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(55388..57148)
                     /gene="pyk"
                     /locus_tag="KR440_000054"
     CDS             complement(55388..57148)
                     /gene="pyk"
                     /locus_tag="KR440_000054"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBH0697129.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEILTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSATDITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(57200..58159)
                     /gene="pfkA"
                     /locus_tag="KR440_000055"
     CDS             complement(57200..58159)
                     /gene="pfkA"
                     /locus_tag="KR440_000055"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBH0697130.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(58368..61937)
                     /locus_tag="KR440_000056"
     CDS             complement(58368..61937)
                     /locus_tag="KR440_000056"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBH0697131.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(62094..63041)
                     /gene="whiA"
                     /locus_tag="KR440_000057"
     CDS             complement(62094..63041)
                     /gene="whiA"
                     /locus_tag="KR440_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBH0697132.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            complement(63058..63483)
                     /locus_tag="KR440_000058"
     CDS             complement(63058..63483)
                     /locus_tag="KR440_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBH0697133.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(63552..64658)
                     /locus_tag="KR440_000059"
     CDS             complement(63552..64658)
                     /locus_tag="KR440_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBH0697134.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(64670..65527)
                     /gene="rapZ"
                     /locus_tag="KR440_000060"
     CDS             complement(64670..65527)
                     /gene="rapZ"
                     /locus_tag="KR440_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBH0697135.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIDEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(65558..66271)
                     /locus_tag="KR440_000061"
     CDS             complement(65558..66271)
                     /locus_tag="KR440_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBH0697136.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYNDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFKLA
                     MKTISDSNLDISLVENIII"
     gene            complement(66275..67189)
                     /gene="murB"
                     /locus_tag="KR440_000062"
     CDS             complement(66275..67189)
                     /gene="murB"
                     /locus_tag="KR440_000062"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBH0697137.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKEESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(67462..68232)
                     /locus_tag="KR440_000063"
     CDS             complement(67462..68232)
                     /locus_tag="KR440_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBH0697138.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
     gene            68550..>69992
                     /gene="cls"
                     /locus_tag="KR440_000064"
     CDS             68550..>69992
                     /gene="cls"
                     /locus_tag="KR440_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBH0697139.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKI"
BASE COUNT        19637 a        14961 c        10647 g        24747 t
ORIGIN      
        1 atattaataa agaatatggt aaaaatttaa ggcttaaatc tttcgcatct ttttgcatat
       61 cagctgtaga aacaaattgt agatttgttt ttttaccatc attaagcttt atttctcttt
      121 cttcaattat caaagaatta ctatcacttt ctaaattaac atttatatta tctttgattt
      181 gtatttcatt cttattattt ttttctttta taaatgcttt tatttcacta ctcttagaat
      241 aaagctccaa agtttgttct atatatttta tttcttttcc attcatatta ctagaaattt
      301 catttgccat attatgaatc ttttcttttc tcgtctccaa atatgtttta ggaaagataa
      361 aaaaaactaa tgaatgaact aatataatta ttattccaag aacagaaaat atttgtatga
      421 acatttttgg aaatatcttt aattttttca ttttctctcc aatttatatc ctacatttct
      481 tatagtagtt atacagtcta attgtaattt ttttctaagt tcttttatat atacatctat
      541 tactcgatca taaggagttt cttcgctatc tttccataca taatcaataa tttgcattct
      601 tgttaacact tgtccattat tatccaataa acattttaat acttctagtt cttttgcatt
      661 tacatctatt tcttcaccat ttattttagc tgtatagcta ttaaaattaa ccgatagatc
      721 attatattca aacttctcta aatgtccata attcttctta attaaagaat ctattctagc
      781 ctttaagacc ggcaatgaaa atggcttttc tacatatccg tctactaaat tagtaaatgc
      841 atcaatttta tattcttcat cactaaatgc agtcaagatt agaattggta aattgctttt
      901 ctttctaatt tctttcaaca cttctaaacc atttataaaa ggtatctgaa tgtctaagat
      961 aaccaaattt atatcgctat taaattttga caaggcttct cttccatctt tagcttgaat
     1021 aacagtatat ccaaattctg aaagatattc acttattccc tctcttatca tattatcatc
     1081 ttcaacagtt aatattttca tatatacctc cacttaagcc tctacactta tattatacag
     1141 catattattt gatatttaaa agccaaaaaa tttttggtgc taaaaagcac cgaaaatttt
     1201 aattattgtc aattaataat tcttttggat tccttcttaa tatattgaat gaagaaataa
     1261 tcaatgatac aagaagtact aaactcataa atataactac ataaattatg aatttaggta
     1321 atacatttat atctaaactt gataatgtct tgttaaatcc ttcagcttct gccccaccac
     1381 ctaattggct agatgcagat tgtctagcta tttgtttagc tatatcacca gtaaccttat
     1441 tcaatatatt attcccaagc ttatcagctg tatattgagc taagaaataa gaaccaacga
     1501 atgcggggat tgatataaat accatctcaa ttaggaattg accaaaaatt tctaattttg
     1561 atatacctaa tgatagtaat actgctattt ctttctttct agcattcatc cataagaata
     1621 ataataatga aactacaact cctgcaaata ttaatgagcc aacaaataat ttatttgaaa
     1681 ttgagtagat acctgatata gattgttgta atgcagggta atttgatgaa cttttaatca
     1741 aattatattc tctccaattt atatctaatt tcccaagatc ttttattaca ctatcaagat
     1801 tcttatcacc ttttacaaag aatgttgcat cttggtatac agctgtatct tctgtattac
     1861 cataaacttt agcagctgta tgaacatcag tgattaatgt attttcgtat agttcttgtg
     1921 ctgcacttac tccaccgcta ttatgcccat cgaatagacc tttaatttct acttctactg
     1981 tttcatctgc acctttctca ttatcagcat caaataaatt agattttatt tttatcttat
     2041 ctccaacttt aagattattt ttttcagcta aatctttatg cattaagatt ttatttttat
     2101 cttcattttc taaatgcttt ccttctacta atttatatgc ttctgataca aattttgttt
     2161 cttttgatga gtcgttaacc ccagttaaca taactgttct tttaaaattc ttcgctcttt
     2221 caggtgattg attcgcaaga gtctcttgag tttcaataat atcataatca actaaatctg
     2281 caacactgtt tattcttttt acataggagt ctatactatc tgtttgaaat atctttttaa
     2341 tatcttcacc ttttacattt cccccacctc taggtgttcc tggatttact tgtctattta
     2401 tctccataga aaaactattt gttatattag caaatgtttc ttttgaagct ctgtccgtag
     2461 catctttaat agataaacta atgagactta aagttgacat agataaaata actaatataa
     2521 taattaatga ttttaagctt tttctagtta cataagcaaa tgcatttttt atcattattc
     2581 aacctccaaa ttcattttat ttactttttt caacttttta ccacttagtt ctaaaatgat
     2641 atccgcagaa tctgctactt ccttactatg tgttacaact attacacatt tatttctatc
     2701 tttagctaat gtctttaata tattaattat ttctccagca gtaacactgt ctagattacc
     2761 agtaggctca tcagctagta ttattggagc atctgatacc aatgctctag caatagctac
     2821 tctttgttgc tgtccaccag ataatttcat aacatttctt tttatttgtt ttttatctaa
     2881 acctagttca aacaagatac tttcatctgc tgatttattt actaatctaa tattttcaat
     2941 tggtgataaa taatctatta aattatagtt ttgaaatact aaagatatat tattttttct
     3001 atgattacta tatcctttct tttgtatatc ttcattctta aacaatattt ttcctgtttg
     3061 aggtttatca agtccagcaa gtaaggaaag aagtgtagat tttcctgttc ctgactttcc
     3121 tactatcgca taaaacttcc caagttcaaa tttttgattt actcctgaca aaactttttc
     3181 tttagaattt gcataactat atgctacatt ctttatttct aatatatcca ttattttctc
     3241 ctaacttatt tttgataata tttctttagg cttcttgatt aatattaatg aagaagcaaa
     3301 tacaactgat aaaacaataa tacttattaa tattaaataa ctttgtccaa gtgttgttat
     3361 atttaacata aaactactat tatttattaa acttccaccg gaaatcattg agtcctctga
     3421 gttaatgaat ccatctacaa ttacttttag taatacattt cctaaaaata aggaagatat
     3481 tatacttggt atcgatatga atattaactc gaatataaat tgcattataa tttgtatctt
     3541 agatgttcca atagataaaa atatacctat ttcataaatt ctttctctta accatagaat
     3601 caagattaat gaaagaacaa ccatcccacc taacataata gaataagtca ttatttttat
     3661 tatatgtttt attccactca ctgactctaa agactcttca aacgcattag aatctttttc
     3721 aacaaaatac tttgactcat caattttaag ctctttcaat ttgtttaagg ctaagtctgt
     3781 agattctgca ctaccagaat acattaaaat tttatttgcg attttattat tttctgattt
     3841 atttaatatc tcttggcttg ttgaataatc tacaaacacc atattttcac taaaatcaga
     3901 agataatcct gtatatgttt cctgtttttt accagaaaag atccctataa ttttaaattt
     3961 atgacttttt atttttccac ttttttcaat atctagtaat tcaagatcaa cttcatcacc
     4021 taattttaga ttgttttgtt tagcaaattc ttcatgaaca ataattgaat tcttatcatt
     4081 ttctcctata tttttgcctt ctttaattgt aaatactcca ctactaaata aaatatttct
     4141 tttagtatta tttgtagctt cgagtgaaac aacattctta aattcgtcag ataaatcttc
     4201 tctatttatt ctttgttctc cgctaactac tttagcatct tttagttttg ctaatccatc
     4261 atattgaatt attttttctt caatttcttt taatttttca atatctttaa attgattaac
     4321 attaaaatat ttaccatctt tttttgttat tgatattgaa gaattagaac tttcatataa
     4381 agccttttct atttcattgc ttgatttcat tattgttaaa caagaataca agcaagaaag
     4441 aactatagtt aatatgataa aaattattaa agttctattt ctctttcttg taatatatgc
     4501 tatagcattt tttatcactt tcctaactac tcctctctat tttcttactc cataactaat
     4561 tgcatccact ggacaagcat ttttacatcg tccacatctg atacactcca gatgattgca
     4621 attctcaact ggatctatat tcatttggca aactttttta cattttccac aattaataca
     4681 cttatcctta tccaatctat atctaaatat tgaaataggg ttaaaaattg agtaaatagc
     4741 tccaagtgga catatatatt tacaaaaagg tctatatata attatagaaa gtaacaaagt
     4801 aactattaga atgattcctt tccaataata caagaaacct atcgcatttc tcatactttt
     4861 atttaataaa acaagtggta atcctccttc cagcatacca atcggacaaa taagtttaca
     4921 aaaatacgga gaaccctgac ctaaaatatc aaccaaaaat aaaggtaata ttattacaaa
     4981 tacaagtaat attatgtatt tcaattttct caaatgttta tcaaacttaa atgtttcaac
     5041 ttttttaaaa aagggaatct tatgtagcaa atcttgaatc aatccaaatg gacacagcca
     5101 tccacaaaca aatcttccca ccaatgctcc tataaaaaac aaaatcccaa ctatataata
     5161 actaatctta tattttgaat ttccaattac tgcctgtagt gatcctattg gacaagaacc
     5221 ttttgctccc gggcatgaat aacaattcat acctggaaca caaattttct ttaaattccc
     5281 accgtagatt ttaccagtct taaacccttc aaagtaagaa ttagtaatta aaaaccaaac
     5341 ggattgaaat aaatgcctaa aacgatctct tattttatat ttatcttttt ttgtactacc
     5401 atttataata tttttaattc tatccaattc caatacactc catacaaatt cttatcgcct
     5461 tatccaagac aactttagtt tctccattac tatgaccata gtaaatcatc gccatactaa
     5521 taaagataat taataaagac aaggcacggc tttttaaaaa cttattaacc atataccaat
     5581 caccgcttta cttttattta ttttttattt tttccaattc tttttcaatt acctgcttcc
     5641 attcctttga actctttgcc ccagaataag tatcacctac aatatttcca ttactatcaa
     5701 caaagaaagt ctctggcata gcctgtatac cattcaatct accattaaag tttgtcttat
     5761 ctggcattaa gaaaggatat gaagcctttg tcttttcatg tattaacttt gacttttcaa
     5821 ttgcttcctt gttttcaccg ttatcatcaa cagtatctgt aacaactcca acgatattta
     5881 cacccttgct tttcatttca ttctgaacct caaccaaatc aggaatttcc tttacacaag
     5941 ctgtacacca agttgcaaat acatttacca tagtaagatc atacttctca aaatctttac
     6001 ttgtaaaatc cttgccattt atgtcctttg ttgatagttt acgtaaatct ttaaccgatt
     6061 ctttgttaaa tgcttctgta ttttctaaat cagttttttc tgatagcaca aaaccatttt
     6121 taggacgttc ttttttatct ataatttgaa tctcagttct ttttaattca tccagaagat
     6181 tactttcagc tccgctattt gtgcttaaat agcaatcgta ttttccatca cttgaaactc
     6241 ctattttagt atgagtatca cattttgtaa ttttagatat tttttcttct gatgtatttt
     6301 tttcgaatat accaatagtc ccaattcttt caagctcatt ttgccaattt ttatatccat
     6361 cgcccatctt ttctataact gcattctttt gttcttctgt cattttttca aatgtcaaaa
     6421 tagcatactt tagttcttta tctataggac tttgatcatc taacatcgct atctttttat
     6481 catccatata ctttttaaat ttgtctgaaa gtttaaattt aagacctaaa tattcataaa
     6541 catattcttt ttttgttttt aaagtataat ctgatggctt aaatgtttca tttttatcct
     6601 tctcattagt tgacatacct gcctcttcag aacttataga ttgtccttct ttaattttat
     6661 tgctttcatt acttttttta cacccaactg tacttaagct caaacataac gtaagtaata
     6721 ggcatgctcc tcttgatact atttttttca taaatttttc ctcctttatt cctcctttat
     6781 ttttacacaa tagcagttta taactacttt caataatatt ctacacacct tttatgaaac
     6841 gttgatgaaa tataattttt tatttttcca aactatggta taataatata aatattatgc
     6901 ttaaagttgt tcttacacga ttagtatatg atcgattagt atatgagcaa gcacagtaag
     6961 tgtacggaca cttacgaaaa aattgatgaa gcagccctct gggatatgct tcttttttta
     7021 tcaattttta acttgttagg gtatacccta agaccccgaa atacattcaa aggaggattt
     7081 acctatggca aatagaatac gaaatgaaag actggaaatt aaactaactg aagaagaaaa
     7141 ggctcttttt gaggagaaac gaaagctcgc aaggtgcaga aatatgagcc actttatccg
     7201 caaatgcgtt ttggaaaagg aaatatatca agtggattta gaaccgttta gagatttaca
     7261 gggcttactg tccaatgcta caagcaacat caatcagatt gcaaagcgtg taaattccac
     7321 cggcataatc tataaggacg atataaacga tatgaaaaag cagattgaat atttctcaaa
     7381 agagttgtgg caaatacatt ctctgcttct taacagaact tctggaggtg attaaatctt
     7441 atggctataa caaaaataca tcctataaaa tcaaccctta atcttgccat ttcatatatt
     7501 gttaatggaa aaaaaacaga tgagcaaatc ttagtaagca ctcataaatg ccatcaagaa
     7561 actgctcata cccagttttt aagaacacga aatgatgttg gaacaaacgg aactgttctt
     7621 gccagacacc ttattcaatc ctttttaccc ggagaagcaa gtcctaaaat ggcacatcaa
     7681 atcggtcttg aactgtgtaa aaagatatta aaggacgagt acgaatttgt cttatctacc
     7741 cacatagata aagggcatat ccacaaccat atcatcttca ataatgtgaa tatggtaaca
     7801 ggcaagtgct atcaatccaa caagaaaagc taccacaaaa tcaggtatca aagcgataag
     7861 ctatgcaaag aaaacaacct ttctgtgatt gatgagtttt acgaaagcta taagagaaag
     7921 tacaaaacaa atggcaagtc atggtatgaa aatgagcaat ctaagagagg tacttcttgg
     7981 aaaagcagac ttcaatttga cattgatcgt cttataaaac agtctaagta ttgggaagaa
     8041 tttctaaaaa aaatggcgga gcttggttat gaaataaagt atgggaaaca catagccttt
     8101 aaaccaaaag ataagacaag atttacaaga gctaaaacga ttggtgagga ctatacagaa
     8161 gaaagattaa aagagcgtat tacagaaaat caatctatta caactccacc tgtcaaaaga
     8221 cgaatcggaa atatcatcaa tatgaatgcc aatgccaaag tgaaagagag caaaggctat
     8281 gaatattggg caaccaaaca taacttaaat acaatggctg agtcagttat ttttatcaga
     8341 gaacatggca ttaactccat taagcagctt gataaataca ttcaaaaaag tgctgaagaa
     8401 agacaagact tacaagataa aatgaaagaa attgacaagg atatgcagct actttccgat
     8461 acaatggagc aggttcatac tgttaaaaaa tatcgagcct actacaaaga atataaagca
     8521 aacccttccg ataaagcatt ttttgaggag cataaggctg aaattacaca ctatgaaata
     8581 gctctttcaa agctaaaaaa atcctattca aagctgccga attcaaagga tattttagat
     8641 aaacttaata aactgcaaga aaaaaagaat acccttattc aagagtattc ttctacaaaa
     8701 tctactatgg acgaacttta tcaaatacga aagaactatg gaatttacat gggtaaggag
     8761 atggaacgat aactctaata ttatatagct ttcttatttt tataatatta aattgaaatg
     8821 cctatcaatg atgcaagcat ttttatacct aaagctaaat caggaattaa agctacggaa
     8881 gtagtgataa aatgcatact ttttaaacaa gtagtcaata cttctttctt cggatgcttc
     8941 tgcttaattt cattttttat attatctaaa ttctgattaa gtattgattt ggagttatca
     9001 tcctccatac ctttaattaa gtcttctaca ttgttaattg ccttgattac ttcttcagta
     9061 ttaaatttat tttcttggtc agcgttaatc gtgcttccgt gttccgcaat attgacttga
     9121 actccagaac tattaacatt gatattatat agagcattat tttcatcgta ccccatatcg
     9181 gtagaaatat ctttcatata attttcaacc tcaagtacaa acggatagac cattctatcg
     9241 ccaaacgcct ttgccatatc ttgatattta ttactgccag cataatacca gccaaaagta
     9301 tatgtaggta gattatttcc tgttataaag gaaaatgctc tatacagtag atacgtcctt
     9361 ttattactat cattgccaag attcaaaact ttacgtccat aagaattact tatcgtattt
     9421 aataagtcat ctaagccatc aacatcatat tctaaactat gcaaatactc ttttatcaac
     9481 aatgtttgct ctatataatt tatcatttct tccaaatttg cattctgttc tccataatat
     9541 gaattcataa catttgaagc taatatccta aattttctac tgatttttct aagttctttt
     9601 ttactaatct ccatctatca tctctccaca aaacgggcaa taactacctt ctaatttttt
     9661 tatactactt attctagcgg ttttgccaca acacaaatat ttcttttctt cataatctcc
     9721 agtttttctt cctatcggaa gttctgcttc ctttttaatc tcttttccac gtttaaactt
     9781 tatactttta ctattcctaa aacttttttc aagattcttt gaaaaatcat ttagcatttc
     9841 agcaacttga ttttcaacta ttcgttctgc taattcataa atatcatcgt ctaaataatt
     9901 ctcgtgtgtt aaaccacaat taggacacca aatatctatt gtgctttcag cctcaatatc
     9961 ttccaccgtt aacataaatt tttcactaca acttggacat tttaattgaa catatccctc
    10021 ttcatccgct ggaacagaaa tttcaaattt tatatcatta gccatttaaa tttcatctcc
    10081 tcacaataaa agttataatt tcaccactgg tagactacaa ggtcgtgcat ctttacgtac
    10141 aataacaaca ctattctcag cacgtgtaac agcaacatat aatctagcct tactttgttc
    10201 ttttagagat gtgttattgt taataatcca atcccacatc ggttgagtcg gatatataag
    10261 tactcgttgg aattccaagc cttttgattc tccaaaatta taatatggat attcattatt
    10321 gacggatgtc cttttatttt ctctcaactg cattgcatta aatttctgca aatacttctc
    10381 tacatctgct ttatcaatta tcataacacc atctgattca tcttcagctc tacgcacaaa
    10441 atttgtagat gttctctctg gatacagact attcgcaaat ttacatataa tatcattgtt
    10501 tctataagta caattaagtg tcgtttcatc aattcttaca tctaattttc tgcactcatt
    10561 ttttaaaaac tcatctattt tcccacaact atatttttta tatttttttg atgagtgagt
    10621 tgaatatgtg cattgtctag gatccccgac cattatcact tcaattcttg aagctattaa
    10681 acactttaat atttccaaat cataccccac catatcttgg acttcatcaa catatataca
    10741 gtcatacatt ccactgattc tttcaaaaac ttttccatta catttatcat ttaactctac
    10801 aacacactct gataatttat cagtatatac cctgttatct ttggcaaaat atttgttgga
    10861 tgttttcttt acgtacgtat tgttctgtcc atttacaaat ataattcctt caattcgttc
    10921 cttaaatcta cttccttgat aaggtctaat accatctttt aacaaaaaag aaaaccatgt
    10981 agaaactgta atattattag gaattacacc aactttattc ttaattcggc taattatttg
    11041 ttgctcattt gtaatagtat atgttaggat tagtattttt ttatgagtat taagagaatc
    11101 ctctataatt tttcttgttt ttccagatcc agcagcagct atatataatt tcccactcat
    11161 attagaatac cctcctttat atattgtgga aattctatag ctttatcact ttcaaaaatc
    11221 tttaaagcaa cttctgtttt atgccccttc atatatttaa gtaaatcatc tggatcttca
    11281 tatgatttat tcaatatctc gtttaaaata gacatggaat tcgatctata tatacacggt
    11341 tccaaagtat tatagttgta attttccaaa gaaccattat attcatgggt actatcatca
    11401 aaatatatat ttatattctc acaacaatta tctcctaaat aatcgctata ttttctattt
    11461 agtgcatcaa tatttccatc attatcggtt atcactgaca cacgtaattc tagcaatttt
    11521 gcgatttcca aaaatcttaa aaaagaaatt ccaaccgata ttatatctat tccatcatta
    11581 ataggcaatt taccattatg agagtccata tatgctcttt gaacaattag ttcgtccgaa
    11641 tcaccttcga ctaaaattgc tttatttgat aataataatc gtaacgtatc ataacctgca
    11701 attcttttaa aaaataaatt tgtttcattt gataaatcac ctagccgtag tgtttttcct
    11761 cgatttagta aaattaaatt ttcaagtcct aatttatttg atacaaaact actatgcgta
    11821 gtaactatta tctgtttttc ttttactgac tcatttactt tatctaggag aatatttaag
    11881 tttgaatatg acaaatggca ttcaggttct tctaaaagca caatctgctt attttctatt
    11941 ttatgatcac ttaatgccaa ttgtgtttta acaatacatt gtgtaccttt accaatgtta
    12001 ttatatggta tttcatttaa ttgggttacc aagctgcttt cccatgaagt tttcgtaccg
    12061 aaatctacag aaattgtcaa ttcgttattt gatgtttccg ttaattttct atttatatta
    12121 gcaatattct catctctaga aaattgttct ttcataagcc tatgcgcttg cgatatatta
    12181 ataatttcat tatcatctaa cagattcttt actattctcg atatgtatat atctgaccca
    12241 ctggtaggag tatagcttgt cgtatcaatc attgcagact taattggaat gctccttgca
    12301 gttatatttt ctctggcaaa agacatccaa cttacctcat aatattcaat tggcaacgat
    12361 gttatcttgt tcttacaaag cattttatac tcattgctat atttttcatc aaaactaatt
    12421 gataaacaaa tcccaggggt gtctttagtt ttttcagaat ttccatcacc taaaaaactt
    12481 ggcatttctg catcaaaata cgcttcaata attattttag gaggttctac attcactcct
    12541 tcgttgactt ctctaatata gttttccact atattcttat tgaaaaggta ctgagtaagt
    12601 tcattcctaa tatttttccc atgataagtt ccagttaaaa ccagattaat agcttccaaa
    12661 attgtagatt tacctgttcc attattgcca actaatatat taatcccatt ttcaaaatca
    12721 actataaacc aatctgaaaa gcatttaaaa ttctttattt ttaatttcct aattgacatt
    12781 ttatccccct tctttatcta atataaaaat catgatttta gaatataaat attgataact
    12841 atataaatac agttatgagt ttcctgttta atcacaatca taaatttttt atgctacatc
    12901 ccttaatatt ttttcaagca ataatttaac aataagtttt atgatcctct agctcctgaa
    12961 aataaatcta tgactgtaaa cttagactcc cattctcctt acatcttcaa taattacgca
    13021 ctccataata tcttcaatat cacaatccaa agcattacaa attctaagca aaacatccgt
    13081 agttacattc tgtccattct tcattttgta aaatgtactt ttactcactt ttgccttttc
    13141 catcaattca ttatttgtca tatctaaatc aatgagtttt ttgaacaatc ttttataact
    13201 aaaaaccctc attcttatcc tccgttcaag aaaactatat ctagtatatt ataccacaaa
    13261 atatactttt ttcataaata tattctcttt aatcgaactt gcataaataa aaaagagccg
    13321 gtgcagtcct aagaccacac cgaccgtaaa tttatctctc agcttctttg cttgcttctt
    13381 tcttttcttt cggtttttcc ttatcttcag cctgatattt tttgatagct ccaagtatag
    13441 attctttctt ttcttcctgt cctttcatct gttcctttgc ctttaacagt tttgaagaaa
    13501 gtatcctgat attgctatgc tccttgccgt tatcatcaat ggaagttctg atctgcccaa
    13561 agagtttgac aaaatctccc tgcttaaagt cctttggaat atcactcttt tctccataaa
    13621 cggagcagtt gtgatataac ttatttccct catcatcttt agatactacg gagaagtttg
    13681 ctaccttaaa ggtttctccg ttcttatttt ccctttcaat tacatcaact tctccaacga
    13741 tatttccgac aatattgata agattatcct tttcttcccg aacataaagc aggtctttta
    13801 tctgcccctg tccccttaaa tcctcaatca tatagtcaaa ctcctcatgc agcaaattaa
    13861 ggctatcatt gtccatataa gcgtcatata gcttatctaa agcactttca tcattgatac
    13921 ccttttccat gcttataatc gctttaacaa agtctttatg attatcactt aacatatctt
    13981 caactctatc tctcattgtt ttgtaatcca tgtttttatc tcctttcatg gtaaaaggga
    14041 ggttgccctc cctttatctt aaaaattcct gctcttttat tttttgtttt tcttctgttt
    14101 tactttcttc ctgataggct cttatctgtc ctaagataga cggcttatca tctgattttt
    14161 gtgtggtttc ttctttgcta tgaagattgt cctccacatc ataagttgac tcaaaataat
    14221 cactgtcctt aaaatcacta tcatatctat ccggaatacc gtcattatca aggtcttttg
    14281 caaacggatc atagaagtta ccctcatcgt caatatcaag tcccgttgct actcttaaat
    14341 cttcagaatc aacataaacc aaatcactaa atgtagataa ctcaatttca tttttcatat
    14401 ttcttagagc ttcattttct cccttatttt cataatcaaa gctctctgtt ttaataggcg
    14461 tatcatcaat gtactgtgtc caagttttct cctctaaatt aagctcatat tgaataccat
    14521 gtctttcatc cggtgtattg gtataggcga taccgatatg ctttaagtca ggatatagag
    14581 tatgaaactc atcataactg tgattttctt catactctct gttacagaag tcaatgattg
    14641 ctctttttac atcttccaca agaggattat catttttctt ttcttccacc tctcccatat
    14701 cgagcagctt atttaactct gcaagtctta ataccttagt tttcaactca tctgcctttt
    14761 caaaaggctt ttttaattcc tctttggcat tttcaagctg ttcttttgta cttatgagct
    14821 tttcttcaag catatttagt ttctccggca ttttctcaag ggcattatcc agtcttgtga
    14881 tattaccgtc cgcacttgtt ccaagctctc ctaaatgctt tgcaacaccg tttaagctga
    14941 aattatgaga attagtaaag aagttatagc ttacctctaa atccatattt ctatactttc
    15001 ctataacctt gctttcattg atttttacct ttgaaatagc ttcaagtaat ctttctccgg
    15061 ctaatttctt atcggtaatt ttttctcccg caatggtaat agaagtaaac ttttcttcac
    15121 cttccgcttt aggctctaca ttggatatat cttttttgac agcttctatg agcttttctg
    15181 ttcttgcgat ttcttcagga tagttttttg ccaccttatc ctctaatctg taacggttag
    15241 acttatagtt tgcttcaagc attttaagtt ttgtaacctc attatccaaa tccatctttt
    15301 ctttaatctt tggatcaccc gttgccaacg ctttaatttc ggcgtaattt aagctgcttt
    15361 cgtccacatc ttctgccaca cgaacaggag tcttactggt cataatctgt gaaatgaact
    15421 tttgcttatt ctctatcgtc tgccaaaggt aggcatcaaa ggtattctct gtaacataac
    15481 gatagatatt taccttctca ttttcatttc cctgccttac aattctaccc gcacgctgtt
    15541 caagatcagc tggtctccaa ggcacatcca aatcatgaag tgcaatcagt ttattttgca
    15601 cattcgttcc ggctcccatc ttctgcgttg aacccattaa gatacgaact tcgccttttc
    15661 ttacctttgc aaagagttca tccttttgct tatcagaatt cgcttcatgg ataaaggcga
    15721 tttcttcttt cggtattccc attgctacaa gtttttctct aatatcatca tagatattaa
    15781 attctccatc tccttttgga gtagacatat cggagaaaag tagctgtgtt gacctatttt
    15841 cttttgtctt atcccaaata gcaaatacat tttttacgca tacatttact ttactatcag
    15901 ggttatcagg aagtagggga ttgattaaac gctgatctaa ggcaagtttc ttaccgtcat
    15961 tggtaatctt aagcatatta tcaacatccg gctctacaac cctatttctg acatcatccg
    16021 ctctttcaga aaggctcttt aggatttcct tttgttcttc agaaggctca gttttaataa
    16081 cttcataatt agcttcaggt gtaggaaggt taagcatatc cgcagtctga atatctgcta
    16141 cttccttgaa catactcatt agttcaggca agttatagaa ctttgaaaat ctcgttttta
    16201 ccctatatcc tgttccttca ggagatagtt caaaggagct ttgtgtttct ccaaaagtgg
    16261 aagcccaaga atcaaaatgc tctaagtgat ttttctttaa actctcatac tgaagatacc
    16321 tttgcattgt ataaagctct gtcattgagt tacttacagg tgttcctgtg gcaaaaacta
    16381 tgccttttcc tcctgtcatt tcatccatat atctgcattt cataaacata tcggaggact
    16441 taaaggcttc tgactgcccg atacctgcaa catttctcat tttggtatag agataaaggt
    16501 tcttgtaatt atgtgcctcg tcaacaaaga gcatatcaac tcccagttcc tcaaaggtaa
    16561 ttacatcgtc tttcttaaaa tcatcattta atttctcaag ccttgtttcc agttttttct
    16621 ttgtcttttc aagctgtttt actgtaaagt tctgatttct gtcatgcttg tattcttcta
    16681 catagtttac gatttcatta atctgatctt ggatgtgctt ttcctgatat tccttactca
    16741 tcgggatttt ttcaaactgc gtatgcccga taacaaccgc atcatattcc cctgtggcaa
    16801 tctttccgat aaatctcttt ctgttcttcg gttcaaagtc ttttttatca gccaccataa
    16861 tattagccga cggatatagc tgcataaatt cacgaccgat ttgtcctgtt aagtgatttg
    16921 gaacaacaaa caaagactta ctgcacattc caagtctttt actttccatt gcggaagcta
    16981 ccatctcaaa ggtctttcca ctgcctacca catgagcaag caatgtgttt cctccataaa
    17041 gacttcttgc tatggcattt ctttgatgag gtcttaaatc aatttctgta ttcattccct
    17101 caaaagaaag attgcttcca tcatattctc tgtttcggat tgagttaaaa cgctcattat
    17161 acagttttac aagacggttt cttctttcct gatcgttaaa tatccagttc ttaaattctt
    17221 cttttaatag ctcctgcttt tgtcctgcaa gcatggtttc ttttttattt aagacagaag
    17281 ttttagaacc gtccggattt ataatctggt caaatacctt tgtttcttta agatttaggg
    17341 catcttcaat cagcttatag gcatttaccc ttgatgtacc aaaggtcatt tctgcaaggt
    17401 catttccttt atccctgctt tttccctcta cattccattc acttgtaata tttgaaaatt
    17461 taaccttaat atcccatctt gcatatcccg gagtttttaa agtttcaaag atgaattttt
    17521 caatatcttt aatcggtatc caagtcgcac caagacgcac attgatttca cttgcttcta
    17581 attcctttgg aagaactttg gtaagctctg ctttttgata ttccaaacga ttcatttcat
    17641 agcttatcag ctctttttct tttccattct caacaaagcc aagatgagga agctctcgtt
    17701 ccgtttgtct taactttgca aggtagctgt ctacaatggc aatcttatct ctaatatttc
    17761 cgcttaggta ctcatccttt gttacatagc cgtacttata tgaattactg ccgtttgcac
    17821 aagcgaaagg caaatctcca tcttcaaggt taaaggataa tggtctataa aagttttgtt
    17881 cttctctgat gtttagataa atttctcctc taagttcttc tatcaaagtt ggtctgtctt
    17941 tacctgtaag gctttccata tactcaaaat ccacatagcc tttttcagat accgataaga
    18001 caagagcttc aagggaagtg tccacatggt ctatgacttt ggcttttgta atggttctct
    18061 ttgagaaaat atcccccttt gccttgacgt tttcttcttc atcaagaatt tcaatggaag
    18121 atacaagagg gaaattgcta tcctctttta aggctctggt attgcttaaa ttatttataa
    18181 agccatgctt ctttgaaaag ttgtcataga cttcattaag cttttcttga gctttcttta
    18241 cctcatcatc ggaatagtcc tctttctgct tgtagataac atctttaagg gcagtattca
    18301 gttcaagata gtccttaatc ttttccttat tcttatccgt tatctctttc ttgatgaaaa
    18361 gggagttttc tctgtaatag acttcatcat caataacggt ataggaaaag tttttgacat
    18421 catccgttgc aggaattgaa gtgatttcat catctaaaag ctctatttct tcataagttg
    18481 atcctttgga tatttcttca cttgcttttg tcagaagctc ttttaagtcg gcattttctt
    18541 tcggcaggca cgcaagtgtg tttccaaatc ttcctgaaac ttcacgcatt gtgccgagta
    18601 cctgctcagg atgatctaca aaatatttgt tataggtaag accattttca tcttcagcaa
    18661 ggtgaatcca gtcctcatct ctttctctga tactgtccct tttctttagg aatataatat
    18721 ctgaggttac ttctgttccg gctatgccct taaaggtatc atttggaagt ctgattgccc
    18781 ctaaaaactc tgctcttgct gcaagatagc gtcttacact ttcatccttt ttatccattg
    18841 ttccacttga tgtgataaag gcgataatac cgccgtttct gactttatca atggatttcg
    18901 caaagaaata atcatggata aggaagttat ttttgttata ctccctgtca tttaccttat
    18961 attctccaaa gggtatattg ccgataaccg catcaaagaa gttattggaa aaggaggttt
    19021 cttccagtcc tttaatctgc acttcacttt cagggtatag tagttttcca attcgaccgc
    19081 ttaccgagtc aagttctatg ccataaaact tggacttatt catttcatca gggatattgc
    19141 ctataaagtt tccgatgccc attgatggtt ctaaaatgtt tccctgttta aatcccatat
    19201 ccgaaagcgt cttatatatt ccgtcaatta cggttttcgg tgtataaaag ctcgttaaag
    19261 tagattctct tgcagcttcg tattctgcat gtgataagtt ctcatttaga aaagcccttg
    19321 cttctttcca ctgaccttcc ttgctttcat caaagacttc ggaaagtccg ccccagccta
    19381 cataccttgc taaaacttcc tgagctgtac tgtcaagctc tctttgtccg ctctccactc
    19441 tgttaagcat ggaaattgct tcaaggttat tatttaacct ttcgcttgga cttaactttt
    19501 cagggagcgt ttcttctgta attttaaagt tatgagcttc cgctttcctt atttgagttt
    19561 cctcagtagt ctgctctggc tgtttatatg tcaggttttc aaataccctc tcaaggtcgc
    19621 tttccagacg ataaggaatg acatcgaagc ttgttatcat cccgccaaaa tactcggtgt
    19681 tgtccttcac cgttaccgta tttaggttct ttctcatatc atcaaagcgt gttatggtat
    19741 ggtttttgtc cttgtattta acttcatcac cgacaataaa gttcggtctt tcaagactta
    19801 ttttattcag caagtcctca ttatctgtaa agtctacaat cggaatttga tggtttccac
    19861 ttcttaccgg atcaagccac aaatcatatc ttcctgtgat ttcattttta gaaatttctc
    19921 tgactttata ttcttcatga ttaaaataga cggtatcgcc tgctttaact gcaaactctt
    19981 ctaaaaggct gtcctctttt tcattcagtt ctacttcttc tctttctttg aggtaatcaa
    20041 ataaggtagc ttgaactgtg ccacgctttg tagcttcttc tgtttcttca ggtgcttcaa
    20101 gctctacttt atcttcaaag ctaagttctc cattaaagac aaactgaagc tcctgctcat
    20161 ccatttgttt tataagctct atatctttaa gttctctaaa ggtctttgga aaatcctcta
    20221 aaatgagcct ctgggcattt aattctttca gttcctcaag gttaaaatat ccccattcag
    20281 gctctattcc aagaacaagt ccaaaggcat caccgctttc cctgtcatac tccgtcatat
    20341 accaagtcca atttgaacga aaaggaataa tatatgctgc atgaacctgc ttatccgcta
    20401 aatctacatc ctcctgtgcg taaatctctg gcactctttc aagaatttca tcaggcatta
    20461 gatttccagg atcattttta gaataatatt gcggttcttt tacttcttct atctctgccg
    20521 tactttctgt ttccttttcc tcaaaataaa gttttttaga aagcagctca tcaatatgct
    20581 ttgcaacact tgtccaagta aggaacacat cattacaatc attcttctgt aatttaaaac
    20641 cctttgcatc gtgccattca tcacttccca ttgcacctga cacaacgtga gaatgtcctc
    20701 cgattccata ttcatctttt aggaaattgg ctttttcctg caaagtatga ttttctttaa
    20761 aaaacttggt gattcgttct tttcctctat caacgccgct tcctcttgaa aggctttcaa
    20821 ggacttcatc ttctgtaata aagagcttta ccttcggaag ttctgtcaga ttggtactat
    20881 attcctttcg ttgcagctca agttcttgca acttctgata aagactatct accttgtgat
    20941 aatgatacct taatacattt ctgctttctt tgtatccttc taaaaacctg ctatattctt
    21001 tgattgtttc tttcagatat tcaggatttt ttaatgcttc agataatctt ttcgtttctt
    21061 gcggaaagcc accacctctt tcaaaagagt tgaaatatcc ttgctctatt ccttcttcgc
    21121 ttaaatcgtg cgttagatac cataaggatt ctgaaattct atccctttca tagtcctgtg
    21181 cctctaaaag ttctacattt gtagcaaact cacctctgtc aagaagttca tttatcctac
    21241 ttgctgcatc actccaactt aaaatttgtg tattatcctc tcttgccgat gttccataag
    21301 ctaaatgaat acctttatcc gaataccagg aagatacttc tctttcatca atgtaaaatc
    21361 cgtttccgcc tttaaaggta tctttgaggt attctcccaa ttcttctatg ctcttgcctt
    21421 ttgaaaactc agcaataaca ggaagcctac cgccatcatg atttcctccg ttaataagga
    21481 cggtatctat atccttctca gttagcggaa tagtaaggct tatctgtcca taagaatttt
    21541 cgggtaaaga aaaagaagcc ttttcagctt ctattatccc tttatccgta ttgtctttta
    21601 aatctccact acttccttga tggtcatttc tttgagagct gaaatcatcg ccccatactg
    21661 cggattgttc tcgtcctcta tcttccaagc tgctatcaac tttggcttct ctgctctcat
    21721 aaactctact gcctgtttct gaatatccat caggtgtcct gtcagcttct tctccttgta
    21781 tagatccaaa agcatctcga agtggctctg ctcctcgctc tgtgaaaggt aattcagcct
    21841 catcactgcg taggtcggat tcggatactt ccttataaag tccgtctgtt cctccaagct
    21901 gtttagcgta ttctccctga ttttttctat tatctcgtcc gtactctcca tttcggagaa
    21961 ctcgctcgtt ttcatttctt tcctgatcat ctcgtcgaaa tacattttct tctacctcct
    22021 ctaattcttc tttaatttta ttatatcccg cttcttttcc tattaaaact tctttttgaa
    22081 gctcaagctc tttgcttttt tgaatggttt catcaataat ctttccgcta atatccgata
    22141 cactttcacc aaggctcatt aaggatatgc tgtcaaatct ttgaaaattt tctcttaaaa
    22201 gctcataatc catcggataa tctaacttaa atcttgaagc taccgcatag cttaccgagt
    22261 cccttacaaa cttggtaaat gatattctat cctcatccgc tactctaagt tcattcatta
    22321 aggtatctat tttttcatca ccgtagagtc tgcttaaaga aaagatgttt tcaagtgtgc
    22381 tttcactttc ctcatagccc tcgcttttta tcatttcctt taagacatct tgatgggctt
    22441 ctttatcaaa tctccaaaga ttgacctcgt tgacatccct gttttttgaa actgtctggc
    22501 ttatatcaaa gatatagtcc acctttttat atgtgccgta atcctctaaa attggtatcc
    22561 ctttctgccc tcgcataacc gttcggttaa agcgttccct ccagtagtcg aacttggcac
    22621 aggctgtcgc ttcaggattt ttatcatata tacttaattg acttctaaaa tcataccttt
    22681 gattgtttcc gacaactttt aggagcttta gatattctgc ttcattatac aaaacatctt
    22741 gttttattag ctccaaaatg ttatgaaaat catttattcg catttttacc tccttctttt
    22801 ttcaaacaaa aaaggcggtt agattttact ctaatcacct tcgctatata ccaaatatta
    22861 ttactctatt tcaatttttt aattgattca ctttcagata gtatcttcat ttgttcaata
    22921 gctattgcat ttaatttttc caatctttca gattggctca tgccttcatt tataaatacc
    22981 gcattaagat tctcaagatt agaaaggcag acaagctgtg atatatccgc ataatctcta
    23041 atattgcctt ttaaatccgg attttcatca cgccactcct tagctgttat tccaaataga
    23101 gaaacattaa gaatatccgc ttcacttgca taaataaaat tgattttttc ttttgaaagt
    23161 ttatccggta caagtttgtt tttaatggca tctgtgtgaa ttctataatt gatttttgcc
    23221 aaatttctct taatatccca tcctaacttc ttttgttctt cttcctttag gcgttcaaat
    23281 tccttgatta aatataattt aaagtatggc gatacccaag aagcaaactc aaacgctata
    23341 tctttatgag cataagttcc accatatctt cctgctctcg caacgatacc gatagaatta
    23401 gttttttcca cccattgctt gaccgataat ataaaacggt taaatcctgc ttcattttta
    23461 attccctcga attcggggga attaaaattc gtgttataca tttcttccca aatacctaaa
    23521 aactctattg tattcttgtt tctgagccac ttttcaatca aagcagaacc gttctcaata
    23581 tttctaacca tatccgttaa agaaatataa tctctattgt ctatggcaat aatgctgatt
    23641 tctgaacctt caacatttat tttagacata taatcacctc tcaatcatta aattgattac
    23701 tttgcattat agcattttta gagcattttt tccacgctga aattagtgct gtcttacttg
    23761 cgatatgtca aaaccgcttc ttttatctca ttttcaatcg catctaaaaa ttctttctta
    23821 gaggatttac cctgagcaat atcagataat tccatttccc actttgctgt cgtttctgcc
    23881 gacttaaagg tatctgctac aatcgttaca aggctgattc ctttatgcgt tgcaattaga
    23941 ttcttcttat ctctttcgac aaatctctta aagattagat tttcaataat tcctgccctt
    24001 gttgccggag tgccaagacc ttttctttcc acctctacac ctttttctaa ggcatcattt
    24061 cccgcaatct ccatagactt taagagcgta tcttcagtaa agtgtttcgg aggttgggta
    24121 aatttttctt taacctcttt attttcaacg ctcaaaacat caccaacact tacatcgggc
    24181 aatacagcat cttcactctt ttttgatttg tattctttaa ggtatttgct aaagccttca
    24241 tccttaatta ccttacctga acttgtaaat tcaaaaccgt caaatgaagc tacaatcttt
    24301 gttgtatttt caataagcgg atagcctaca cttgcatgaa atttatcgga tataaggaag
    24361 taaacttttg cttcacttaa aggtaattca gaaacatctt cttttactga actgattgtc
    24421 ggtatgattg catgatggtc cgtaaccttt ttagagttaa atactacctt gatacgctct
    24481 gtatcaaagt catttttacc caaaatgttg ttgactgtac tcacaatcat atcctctgtt
    24541 aaacatctgc tgtctgttct ggggtaagta atcagtttct tttcatacag gctttgagta
    24601 tagtcaagtg tctgcttggc actatatcca aaatatttat tgcactccct ttgaagcgta
    24661 gttaggtcaa aaggtaaatc aggctttgta attttttctt tttgaataac atcagtaatt
    24721 tcaatcttat cgcctactaa acttataagc tgttcagcag caacttcatc atcaattctg
    24781 tctgtggaga gtgtaaatcc attcatagaa agctctactg tgtaatattt ttctttctga
    24841 taatttgcta tctcatcatc tctttttaca atcatggcaa gggtaggtgt ctgcactctg
    24901 ccaacactgt aattttgctg atacagacaa gagtagagcc tactaatatt cattccgact
    24961 aaccaatccg caatagctct tgcctgtgcc gattcaaaga gattatcata gtcttttccg
    25021 tctgttaggt tatcaaaacc ctctttaatg gcactatctt ccattgaaga aatccaaagg
    25081 cgtttcatct tctttttaca attcacttga ttatatacaa gtctaaaaat actttctccc
    25141 tcacgccctg catcgcacgc attgataacc gtatcaacct ccttatcatt catcagcttt
    25201 ttaaggatgt taaactgctt cttggttgcc tttgctactt catacttgta gtcgctcgga
    25261 ataatcggta aatcagccat attccacttg gcgtactttt catcataagc atccggattt
    25321 gccatttgaa ttaggtgtcc aacgcaccag ctcaccctat atccgtttcc ttcataatat
    25381 ccgtctttct ttttatttgc tccaataacc tttgcaatgg agattgcaac actcggcttt
    25441 tctgcaataa ctaacttcat ctgtgttttc ctcctgttat aaatcaaaat agggcgaaag
    25501 atgttactct cccgccctaa ctttgcaccc aaaataatat gtttttaggt gcgtttattc
    25561 ttcatcttct acttcatctg catcatttat ttcttcctcg ctttcagatt cagaaaagaa
    25621 atcatcatct tcctcctcta aggcttcaag ttctttatct tctttctttt tcacaacctt
    25681 aaaatagtag cctgctccta atgcaccacc tactacaaga agcaaaataa tataggttcc
    25741 taaattactc ttttcgtcct tcttctcagg ctttacttct tccttaacag gttcttcttt
    25801 tactacttcc tgctttggtg cttctttttt ctccaccatg ttaagcaaat catcttcaga
    25861 tacttccgta agaagcatta cattctcact atcttcatca tgattgatga ttaaatgaaa
    25921 agtcttaccg tttttcgttt gaaaggtaat aaactgcctt gcatctgccg aatactgatc
    25981 cgtttctttg ttatcgctgc tatctccatg atgaatcgga tactccttat ttgcattatc
    26041 tttattctcc gtaacggatg ctctcgcctt tgacggagat gaagctactc ccttgttggt
    26101 gtttacactc ttacttgcag accccattga tgaatcctga ctgttattcg caggagcttt
    26161 tgccgtcaac ttattgggat agcgaacttc cttttctttc tcctttactt ccgttttgcc
    26221 tttaccggta tcctttgtag tattacctga gcttaccgaa tccgatgagc ctttaccggt
    26281 actacttaca gaagtctgtg gtgtttgagg agaaatacct gaacttgtct taattccgga
    26341 aataggacta atcggtgtaa ctgattgtga tgttacagga gctttatttg tttcagttgc
    26401 ctttttctca agctctttca ccttttccgt cagcttatct atttcctttt tcatatcttc
    26461 tgataagtcc ttatttccct tatctttctt cattttttct ttcaggctat caatttcatc
    26521 ttcaaggtct ttaattttat tcttttgctt atcacttaat ttgtctttgt ccttaagttc
    26581 gcaatttaac ttatcaagtt tatcctgaag ctcttttgcc tctttttcta tttttgaaat
    26641 atcgtcttta gaaagctctg tctgcgttcc cttatcttct gtctgagtgc attcatcctg
    26701 ctttggcttt tcttcttccg tctgcgtttc cttatcctta acctccatct ttgctacctt
    26761 acggataatc tcatcttttc catctccctt tacctcataa aacagaaatt catcaatgaa
    26821 tttcatatca tctgggagca ttggaagatc tccgctatca ataagttgtc ctttctcagc
    26881 ctttatcttt tcttccttaa agaccttttc atcttcaaag atatacctta catttacttc
    26941 aacttccgtt tgaacagctt cgttttttgt tggagcttca gatttttgct cctgtgcata
    27001 aacaaccgtc caaagaccta aaatacaact aatacttaca attaccgcaa gtgctaccgt
    27061 ccaaaactta ttattctttt tcaaactggt tttcatttgt tttctccttt ttcatttctg
    27121 ctttttgtct gtttaccttt gctatcaaat cgctgattgt gatgttgttc tttctgcaaa
    27181 tggcaatgat ttcttcattt tcaagctctt cttcacgaat gaataaaggc tccaattctt
    27241 catcaatcag agccttttta tcctttaact tctttattct gttttttact gttgtaagtt
    27301 cctttttcaa attgttacct cctatttctt tacatttggc ggaaaaccga atcctacggg
    27361 atggtgctta caaaatgttg ctatccaatc atctaaggta gttactctta ttcgaccgat
    27421 gttatcaatc acttttccat ctccaatgta aatacctaca tgaccatagg taagccctgc
    27481 actgcttccg ctactgctgc tttcaactgc cacaagcatt cccactttta gctttgacct
    27541 gtctgatgta aaggtatagt ttcggtacat atcacaggca ttcccgccta tatatccaag
    27601 tcctgcattt tgatagacct gtgataccca catggcacac cagcctgcac ccggagatgg
    27661 cgtaatgtat gcagcattaa caatcttctt ttgtacttcc gtagatgcct catactcttt
    27721 tccaccgccg atacctccat ttgcactgat aaggtcagaa ttaccaaagg cttctctcat
    27781 attgccttgt gcaaggaaca aggcttcata gtgctttaga ttatcgggat agtttgcaaa
    27841 gacctttcgg ataatactgt ccatctcttt tttatgaagc gttacgatga gttttttata
    27901 ctcataaggt tcttcgtggc tttcggtata ttcattgcca tcttcatcgg tataggtttc
    27961 cgtaaccgtc ctgtatctga tttcaatttc ttccctatac tcaaggtcat acatggactc
    28021 aaataattct tttaatatgg attctacttc cgatacactc tttacctctc cgcaccttga
    28081 tgtaatgtag gataaaagct catggacatt atgaccgatg tattctgtat tatttaagat
    28141 atattcatca tatccaggat aattttcttt cacatagtca acttcacttt gaagctcacc
    28201 ctccatatct gaaaatttct gattgatttc acttaggaca tttggctttg ataaatagct
    28261 tgttgtaagc acagagcttg tagagttcat aaagcctgtc attcccgttc ctgcaaagtt
    28321 aatcacaaaa gtccctaaga taatgatacc tatgaaaata agcattagtc cttttgcttt
    28381 tcggataatc atatcctttg agcttttcag tgttccgata agtccctctt taattcgatc
    28441 tctaagcctt gacttattct cacgagaaat tgctgccttt acctgatttt tcttttgaaa
    28501 tcttttatat gcacttgctc ttttatactc atccgtcttt ttcagttcct ttttggcatc
    28561 acgaaattcc aactttgact tacgctttct gattttatag tccttatttg taaggtcata
    28621 gcctctttta gctcttttct tatcagagta cttctttatg ccatgaatga gcttggagct
    28681 tgtatctgct gtcttttctc ctgcttccac tccttgattt tcatcacttc catgagacaa
    28741 gtaatctctt acagtttcac ttcctttagc aagtcctgaa agggcagata cctttaccat
    28801 gtcttttttt agctttttct tttggtcttt tgataggctg ctataaactt ttccgctgac
    28861 agcatctttt cccgcctttt tatcatcagc cttatttccc tttggtgcat cgtccttttt
    28921 ccttgtataa aggctttcag aatagttctt cctcttgtac ctgctttttt gagtcttatg
    28981 atttttaaag gaattttcta atgatttttc tttatgggaa agattatcct ccacatcata
    29041 ggtcgattca aaatagttgc tgtctctaaa gtcattatcg tatctgtcta taatcccgtc
    29101 attatcaagg tcttttccta aaggatcata gatttttcca tccttaacct ccgtaatata
    29161 atctgatctt cccgtttcaa ttgcggaatt tacgtctccc ttatctgaag ttctatattt
    29221 ttcgtttctc ttggatgttc cataagactt ttcattatca gcacttacct tacttgtttt
    29281 ttcatgcact ttatcctgaa accgttcttt ctcatgaacg attttacctc tgtaatcatc
    29341 gttatgtttt agcttacttt cttcaggtat cgtatctgtt ttactatgaa acatctctct
    29401 ttcaaggctt gccttatgcc tttccttaaa atcctttttc agcttctttc ccataggcta
    29461 cctcacttct tccggcttgg tagtcatctt ctgatataaa attgtatctt tcggaaactt
    29521 atcaatgaaa ggcacaatgg tatttccaaa gaataagagt ccctcacctt catttgaatt
    29581 agtaacatat ctgagctgtg gaagtgagat tttaagtttt cttgcaagta tctccctatc
    29641 tcctgatgct tgattaagca ttaagacaaa gtctgtattg tcaaagatat tttctatctc
    29701 cttactcata agtaggtctt tgacattctg cgtaatacct gttggaatac ctccccattt
    29761 acgaaatctt ttccaaatct ctacggaata ggatgctgtc tgctcatctt ttaaaagcaa
    29821 atggaactcg tcgatatagt accttgtagc cttgtttcct ctgttttgtg acaccttatt
    29881 ccacacctga tcctgaataa caagcatacc gattttcttt aattgacttc caagctcctt
    29941 aatatcaaaa caaaggagct tcttatttaa atccacattt gacctgtgat taaagacatt
    30001 gagagacccc gatacataga tttccatctc tgttgccagc ttcttaccga ccttttcttc
    30061 ctgccctttt aacatatcgt ataggtcttg aagtatcggc atattgtcag gagtaggatg
    30121 ttcaaaatac ttttcataga ttttaggaag gcacctatct ataacggact tttcttctgc
    30181 tgtaagacca ctaccgccta ctacaagctc aagcatactc atgataaagt ttgccttgtc
    30241 tttcagcggt gcatccccat caccgtagtt catgtttata tcaagaggat tcaggtaatc
    30301 cttcgactta ctgctgacct taatgacttc tcccttaaac tgccttacga ggtttccata
    30361 ctctccttcc ggatcgcaga taatcacatc atcatctgtt actaaaatcg catttgccat
    30421 ttctcttttg gcactaaagg acttaccgct gcccggagtt ccgaggatta gaccgtttgg
    30481 gttctttaat ttctttctat ctgccataat caggttatgg cttagggcat ttagtccgta
    30541 gtaaagacta ttacttgaat tgataaaaag ctcctctgtg gtaaatggca taaatactgc
    30601 cgttgatgag cttgtcaatc ctctatctat ttcaatctta tttacaccaa gaggcagcac
    30661 gctcactaaa ccttgttcct gagaatggtc aagtcttttc agcttacagt tatgcttatt
    30721 tgcaatggag cttatttgag caattgtgtt atcgagcttt tgcactgtcc ttgcaaagtt
    30781 cataaagacg atacttacca caaacattcg ctcatctctt gtctgcaagt cttttaagag
    30841 gctctttaca tcctcaccat aggtaattaa gtccgatgga agaatgtcca tatcataacc
    30901 gcttcttacc gccttcttat tttcctcaat tttcatctta tcaatatcag tgttttttcg
    30961 ttttaccatc ttaatggctt ccgattggtc gattgcccta atatgaaagg aaatattgat
    31021 gttatcgtca atatccaaaa actcggataa catcctatct gaaagctcac tggcaaggat
    31081 ttgaaaatga cttgttgctc cgatgaattt gccgaactta aagtatctac tcggtgtaaa
    31141 gttaaactca tcaggcacaa tatatgtctt tgtgctttcc cttttcttta ggtctttgta
    31201 ggaaaattta aatgttttat ttggatttaa aacatcatga agtatcttta gcctttcttc
    31261 tccgtttagg ctttctgctc ttactcccat acttttaaga ctcgataata tatctatctc
    31321 cagtctttca agttttgatg ttgcctgttc taaattatcc gcttccactg taaaggttac
    31381 atactttgat tttttcaatc cgttatttcc ctttacaatc tggcttttaa gcatctctct
    31441 aaattcaaag cgtatatcgt cgaaaccgtc ctttttatcc ggtatctgaa ttgccgactt
    31501 catttcttcg tttcgtccga gctgattgat atatgaaaac tcaatgctga tacttggatc
    31561 aaaagagttt aggaagtttg caaattgatt aaaaatcaag tccctatctt catctaaggc
    31621 aagctgatag ttaatgtcct gaaaggctat acttttactg aagtgctttt catcaagctg
    31681 gcatattccg ctttttaaaa gccttagata gggaatggta tcttctaccg tatatctttt
    31741 cggttctttc ttaaagataa ggtcaaggat tcctccctta tctttttttg acttgcaatt
    31801 acttttcttt aagtcccgct tttgacttct caatgccttt tcgttttgca tgagttttat
    31861 ttgctgaatt tttcttttct tgttcaaggt aaacctcctt tctcactctc ttttgcggtt
    31921 gataaaactt atgaagataa atgtacttaa agtatttctc aaatgtcagt ccgtcttttt
    31981 caaatagtgt gatgaaaaag ataggaagcg tggatacgat aaggaatatg acagctatgt
    32041 catttggcac aaacttcctc ataaataaat agactggtat tccaaccagt cctgcaagtg
    32101 tgaagcctat gagctgcctt ttcgttaggt taaaagcaac ctttgtcttt accctattta
    32161 agtctttagg gattggtaca tatgccatat cttacctccc atcttcttta ccctttgaca
    32221 gctcctcaaa gtgttcatat acagagccga tttcttcctg aatagtcgca agctgttcgt
    32281 ctgtgttctt gttatatctt tgctgtatta ggtaaaaatc gttatggcag cgaccaatgt
    32341 cctctgtttt ttcttccaat tcttccacct tattatgaag cctgatccta tcaattatcg
    32401 ccataatacc tgctccaatt actactataa atactgtttt tcttttgttc atattaccct
    32461 tcctttcttt tagtgtgcat ttaatacgct tttggccagt gttccgctct ttaacatcat
    32521 taaccctagc aaaaccgcat atccaagtat cgtaaaggta cttgtgtgta tatctgttat
    32581 ctgtattgtc ttaactaata ctgcgtatat tccaagacaa accattaaaa agagtccttg
    32641 taaacctatg gcaaacaatc ctttgatata gtttgttcca atctgtcccc actctttgtt
    32701 tcccattgtg gcaaatggaa tggctgaaat ggatgagtaa acataaatct caaacattct
    32761 tccgtaaacc acaagcataa tcacaattga aatgacctgt attgccacct ttataagcga
    32821 ggtttcaaag agtatcatga caagctctcc aagaccttta tcctttaatc catctaccat
    32881 ttttactatc tgatctccgg agataacggc agaagtattg attacccctg ccgctttatt
    32941 taccatgtgc tgtgccacat caaagaccgc cattgaaaac tcaaatgcat gtgatactaa
    33001 ccatacagca atccacatct tgatgatgta cttgaaaaat tcaaaagtat ctgtatcgtg
    33061 catattgttc ttttgcatta ccatattgat aagctcgata caaaggactg ctgtgatgat
    33121 tagccctgct atgggaataa tgacggaatc gttaatgctt ttaataaagg caaatacatc
    33181 tccgttccac cccataggag tttttcctac atctgttgca actgcaccaa ctttatcgtt
    33241 gatgtcaaga aacatggact ctaagtttgc ttgaataccg cccagtagaa gctctttaaa
    33301 gaattcttct atcttgtcga atattccaaa catctaagct ctccttttct tattacttga
    33361 gtacatttgc aagaagcgga atcagcttaa gtccgataag gacaatgcct cctcccgcca
    33421 taagctgctt gatgccctga gatttcgctc ccggattatc attaccatat ccttccataa
    33481 ggttaataac tccccatgct ccaagtccag cacctactgc cataactaaa atctttaata
    33541 catttactgc ctgtgcgaaa aattccataa tttattcctc ctcattttct tctttcttct
    33601 caatcttgtt atatgacttc actacaaagt ttttgtaagt ctttccctct ttttcacgct
    33661 tcttaaagta gccgaataca tgaatcaaat cgcctttttc aaagtcctct gctttctctg
    33721 ctttttctcc gtaggctgca caattgatgt actccttgcc ctttccatat ttttttacaa
    33781 gcgtaaagtt tacaacttct acttcttcac cctctttttc aaaacttgag aaagtaggtt
    33841 ctgccactaa gttggcgttg atgttaatca tttcttgttt catcttttta aatttctcct
    33901 tttcatttca ataaaaaaaa cgactggagt ttttcttttc caatcgctga tactcttact
    33961 atttagtttt cgtttaagtg ggatttctga tccttatcat cttgcctcct gattttgaat
    34021 aaaaaaacag caaatctttt taagacttac tgcattcttg taataaccgt ctctctattt
    34081 aattttgctt ttccttttcg cttcatatag ctttctatgt caaacaaatt cttcttgtca
    34141 taatcctcaa gcagcttata attcttgtgc tttgtaatat cgtatttatc tgaaagaaaa
    34201 ggtctgacac ctctaagctg aaatatacat tttccaccgt ccattacagt aatctcatct
    34261 tgactcatca gttctttgcc acaatgtcaa gtgatgaatt aaaaaaagct atcaaagaaa
    34321 tccttcttcg atagcagcgt attcaacatc tattcacatt tcctgccttg cgttcttcga
    34381 gctgatgaaa tataataaga aatgttgtcg gaggtgcgaa tatggactac atgacgctca
    34441 aagaggcaag cgaaaaatgg ggcgtttcgt cccgacagat aaattattac tgcaccgatg
    34501 gacgcatccc cggtgctgtc aaaatggccg gtgtctggct gatccccaag gaggcagaga
    34561 agccaaagga cagacggtat aacgaaagca ggtgatacag tgctgcatat agcaatttgt
    34621 gatgatgaaa aagactttgt tcagtatttg accgatttac tgaaccaata cagcaaggaa
    34681 acaggacggg acataaagat taccccgtac tatgacggta tggagctgat tgaaaaatac
    34741 gatccaacca tagacctgat tttccttgat attcaaatga aggtggtgga cggacttcat
    34801 gctgccgagc gtatccggca aatggacgaa accgtgggca ttatttttct cactactctt
    34861 acacagtatg ggcttgaggg atataaatac catgcgacga actacatcat taagcccatg
    34921 aaatatgccc ggctcaaatc agaaatggac aaattcttgg agcgcagcca gaaagaagat
    34981 accccctctc tggtgattac caacgatacc ggcaaatata aggtgccgct aaaatcaatt
    35041 cgatatgttg aaacatataa ccgcaatctg atgttccata ccgagcagga aaatattatc
    35101 tgctataaga gcatgaaaga agtggaacgg gagctttgcg acaaagactt tgtgcgctgc
    35161 cataccagct acattgtcaa tctgttctat gtgaaaggca tcaagaagct ggacattgaa
    35221 ttgattacag gcgagatcat tcccatcagc cagccgaaac ggaaagaatt tatggaacgg
    35281 ctgacggact attgggggga tatgctgtaa tgtggttata tcaagtgctc gacataacag
    35341 caacgctgac agaatctttg ggactgtatt tattcagtat ttgtttttgc aaagaaccac
    35401 ggtatcgtgc ttctgtaaat agatggctta taacaggtat aagttttttg tgtgcgtata
    35461 cattgacatg gttttctgaa cttggcgcac tcaaagcgcc ttttcttctt acgatttatc
    35521 ttgtcctttt gaaagtttgt tatagagatt ctgtctatca atgtgctgtt gctgaagaac
    35581 tgaattatat tgcaacttgt tctcgatatt cgcaaggtag cgcggggaaa tatataactc
    35641 gtcgcttacc tttttgcggc tctcgccgta ttcatttctc gcggctttta tagctgcccc
    35701 gaaagcctta aagtcgtaca atggtacggg tctttttgcc attttcattc accctgttac
    35761 attttacagt tcacctttct ttttggatat gtccacacaa ttcatatcat taggttaaat
    35821 acttcctgtt gtgtattgac agtagactga ttttctgata aaataaaatt tatgtaaaag
    35881 gaggcggcgt ttatgttgca tagcatgcgt attagataag cattgaaaaa aaggattttc
    35941 ccattttcaa catgggcttt tttgtcatgc ttattttgcg gatgctatac aaaaaactaa
    36001 cgacgtatag atatagtata gacatagtgc aagctatgcc ttagttttgt tgtactgctc
    36061 tgtgcatttt ttgggtaggg aaaccaccgg ctgtgccggt ggaggtaccc cgtaaaaaag
    36121 ctttagcttc aagcatcgag cgaagcgagc tgctaacagc aaattatcag tgaagaaaga
    36181 cttgcattag aacagcggct gcccgtttac gggctgcggg gcaaatgatg cgggtgaaat
    36241 aaaattcagt gcgtcttgag acgcatgccg gtaataggcc cttatagggc caactcaagc
    36301 caccggctta gccggtggtt atgacttgtg taaaaaggac gagtgaaata taatgcttaa
    36361 aaaatattgg ataaaatgtc cgatttgtaa cggaaagacg agagttcaag tatttcataa
    36421 tactgtatta aaagattttc ctcttttctg ccctaaatgc aaattgacgc atatcattga
    36481 tgtagaaaaa ttagagattg taatcaaaaa tacagaaaaa caaactttta ttatttagaa
    36541 gaaaggatat aaaaatgaaa cgtttaccta aatatacgcc tgcggaagta cggaatgatc
    36601 catacggatt tacttacaaa gaaatgtcgg aagttattgg cgagaatgaa gcaaaagcct
    36661 tatatgaaga attatataag caattaccac gcaaaaaaaa tctatcaatg ttggtaaaaa
    36721 atatttgcaa aagcagtgat actgaaaagt atgtttacga actgaaagac aacaaataca
    36781 ttgaaacggt ttttatcaag cggcgagatg gtggaactgt ttgcgtgagc acacaagtcg
    36841 gttgtcctgt tggttgtatt ttttgtgagt ccgggcgaaa tggctttgtt cgtaatctaa
    36901 catcgtcaga aatcgtacaa cagattatat tgttgcgtcg aaaagtaaac cgtatcgttt
    36961 ttatgggtat gggagagcct ttattcaatt atgacaactt gataaaagca atccatattc
    37021 tccgagatag atatgggctc aactttccaa ccgacggcat taccatatca acagttggtc
    37081 cggtcgatca attaaaaaaa ttgcgcgagg aacatcttaa aattcagttg acaatatctt
    37141 tacacgcagc aacacaatct gcaagaaatc gtattattcc tcacatgcgc atatatgcta
    37201 ttgaagatgt tgttaagcaa gccttatcct attctgaaag gcataatcgc aaaattgtct
    37261 ttgcgtattt gcttttaccg ggtataaatg accggccctc agatgtaaga caacttgcaa
    37321 aatggtttcg gggcaaaaaa gttatgatta acgtgttaca atacaaccca acaagcaatt
    37381 caagaattaa agcaccacag aaacgggaaa tagttgcatt caaacatcaa ttagagcaag
    37441 caggacttga agttactatg agagtttctc atggcagaga gattaacgcg gcttgtggac
    37501 agttagctaa cacatataat aaattcaaaa aaaaatgatt aaaagtgcca gacgcataga
    37561 cgcagagccg ataacgcatt aggtcaaaaa acctatgtgt tatcggcttt tttatttaat
    37621 attgcgcaaa agccgctgtt ccctattata gaatggattg cgggtagtgt attaaagtcg
    37681 ccctttttta aggatacggc ggtatcgggg aggtatcgcc gtgtccattt ttatttactg
    37741 taacccgcct aatgctttgc ggtcaaaaaa agctatgctc cgcaagcggg atattccggc
    37801 tatgaatgtg aactgtcaat acatttagct actgcttaca tttcctttgc gcccgtccgc
    37861 gtcttgcgga cgggcgcatt ttgctttttt caactttttt ctgaaaagcg cactcaagag
    37921 ccatctcccg aacgggtacg gagcgaaagg ggcagagagg acaagccctt aactcccgtc
    37981 ctctactcca cgcgggaagg aggtgaactc tatggagcta tcttcttccg acaaggaaag
    38041 aatacaacat cagtacgacg cattagcaaa gaaaactttg gtcggcgaag cgaaaagcca
    38101 ccgccgcact cttgcgaaac gcgcagcacg cgaagttact ttttcggatt tgagcgaaag
    38161 cgaactcgcg cagcttttca caacggacga atacgaaagc gattatttcc gttttcaagt
    38221 gtccggcttt gatgtactcg tcaaaaatga actgcttgcc gaagccctta acgctttgcc
    38281 cgaaaggaaa cgcgacatta tccttttgtc ctacttcttg gatatgagcg acgcggaaat
    38341 tggcgaactg ctgaatgttg tacgcacgac ggttttccgg cacaggaaat ccgcgcttgc
    38401 gaaaatcaaa cagtatttgg agggaaaagc agatgatgaa taccgttagg aagtctgaaa
    38461 atctgttgcc gttccctgtc atttccgcag cggcaaacgg cgacacaacc gccatgtgcg
    38521 cgatcttgaa gcactacgag ggttacatag cgaaactttg tacccgcacg ctgaaagacg
    38581 acgcgggcaa tacctattcc tatgtggacg aggaaatgcg taacaggctg caagtgcgcc
    38641 ttattacccg cacccttgct tttcatgtag gataatcttt tagcccatgc ggggagcgtg
    38701 tcccctttcc acgcttcccg ttatgggcta tttgtcgttc cgcaaaagca tatccgctgt
    38761 tgatgtgctt ttgcaggccg acaaagccta ttgttctttg acaaagaaag cggcctttgg
    38821 tgcgcagcat aacaggcaaa cgggtacatt ccgtctgtac cgagccagtc ggcgggtacg
    38881 ccatgacagc ttttccccat aggggaaaag ccgagcgaga actaccgcgc cgtaaaacag
    38941 gtttagcagc ttgtgggcga cgacatacaa ggcggcacaa tgatactccc gcgttgaaca
    39001 ccctcaaagt attgcgcggc gcacccataa gcatgggccg gggtgaaact cccgtgaggt
    39061 tgcagctaac aaccgcccgt ttttgccttt tgacgaatat agtttatcca tacaggaaaa
    39121 ggaggttttt ctaatggata aaaaacagac aattacaacc gaacgcaaaa tagggaaaat
    39181 cacctatctt gttcaagcgt tgccgagtga aaaggcaacc gatacaatcc ataaaaagat
    39241 tgagaaactc attgtaaagg atttgcagaa aaagcccgga aatccgggat ttttagcgtc
    39301 cgagtagtgc attaggcggc gggatatggt ataatgatag caacacatta tacctgttta
    39361 ttcggctgtc ggaaaggagg actaatgtta cagtcgaata aaatcaccgc cctttactgc
    39421 cgtttaagtc aagaggatat gcaagccgga gaaagcggaa gcatacagca ccaaaaaatg
    39481 atacttcaac gctatgcgga cgaacaccat tttttgaaca caaagttttt tgtggacgac
    39541 ggattttccg gcgtgagttt tgagcgcgag gggctgcaag cgatgttgca ggaagtggaa
    39601 gccggacgag tggcgacggt cattaccaaa gacctttccc gtcttggcag aaactatctg
    39661 aaaacgggcg aactcataga gattgtattt cccgaaaacg gagtacgcta tatcgcgatc
    39721 aacgacggag ttgacacagc gcgggaggat aacgagttta cccccttgcg gaactggttt
    39781 aacgagtttt acgcccgcga cacaagcaag aaaatccgcg cagttaaaca ggcacaggcg
    39841 caaaaaggcg agcgcgtcaa cggggaatat ccatacggct atatcccaga cccgaacaac
    39901 cgccaccacc ttatacccga cccggaaacc gcgccgattg tcaaacaggt tttcgctatg
    39961 tttgttagcg gcgtgcgtat gtgcgaaatc caaaaatggc ttgcggaaaa caaagtcttg
    40021 acgattggag cgttgcgcta tcagcgtaca ggacaggcgc ggtatcagcg ggcaatgatc
    40081 gccccctata cttggccgga caaaacgctc tatgacatat tagcaaggca ggaatattta
    40141 gggcatacca taaccgcgaa aactcacaag gtatcctaca agtcgaaaaa gacccggaag
    40201 aacgaagaag aacaacgcta tttcttccca aacacccatg agccgcttgt tgacgaggaa
    40261 acctttgaac ttgcgcaaaa gcggattgct acccgccacc gcccgacaaa agcagcggag
    40321 attgatattt tttccggctt gctgttttgc gctggttgca gacataagat gtattaccaa
    40381 cagggcgtaa atatcgagcc gcgcaagttt tcctattctt gcggcgcatg gcgcaacagg
    40441 gcaaggacag gcagcgagtg tacctctcat tatatccgca aaaacgtact tcttgattta
    40501 gtgctggaag atatgcggcg ggttttgcgg tatgttaagg aacacgaaca ggactttatc
    40561 tgtaaagcta ccgagtacgg cgacatggaa gcgagaaagg cattagcgca gcagcaaaag
    40621 gaacttttca aagcacaggc gcgtatgacc gaacttgaca cgcttttccg caagctgtat
    40681 gaggacaacg cattaggcag actgacagat gaacggtttg tgtttctaac ttccggctat
    40741 gtggacgaaa agaaatccct tgccgcaagg atagacgagt tacaacagca gatcgcaacc
    40801 gttaccgagc gaaaaaggga tatatcaagg tttattcaga ttgtcgggaa atacagcgac
    40861 atacaggaat tgacctatga aaacgtccat gagtttatcg accgtatttt gatacatgaa
    40921 ttagaccggg aaaccaacac ccggaaaatc gaaatccatt atagctttgt cggacaggtt
    40981 gataccgagc aggagccgac gcaagttgtc aaccatgacc gccgcaacat ggtagatgta
    41041 aaaagtatcg ctatctaacc ccagcaaaaa aggggcctga aatgaaagag tacacgctaa
    41101 ttatcaaggg agaaatggat tttatcatct tatctccgca ggtgctttct tccctgatta
    41161 ctcaaatcca taacagcccg gagcgaaaag ttgtggtaag tattgagagc atcatgccac
    41221 caaagtttac cgactatctg cttcgggtaa tcaatagcaa ccgctttagc aatgaacggt
    41281 tccgctatcg ctacatttta gaaaatcctg ttacaaagaa agggatgtat gagattttac
    41341 ggcaacagct ttcccgcacc aatacggagc gatttccttg ttttcagacc atacaattga
    41401 ccgacacttt ccagggtaat gtggagctgg acatggagtg caacgatctg ttcttttggg
    41461 cttgcaaaga tacagcagca aagtttgttt acacgttccc ggatggacgg gaggaaacac
    41521 tggtgattga atactgaatg agaaaagccc catccctcaa tggttgagag atggggcaaa
    41581 ctatttacgc tgcaatctcc gggatttccc cgacaaagtt ataaacaatt ctgacttcct
    41641 gcaccttctg accgtccacc ttcttaacct cgccgatcag gattttgctg ataagccggt
    41701 tcagtacgga ctcgtccagt tccgcaatgg cggcatagcg ccggatttcc ttgataaagg
    41761 ttcggacttc gctttcctgt gcgtccgccc ggctctgcat caccgccaga tcgtgcaggc
    41821 gtttctgatt ggcttcctgc tcctgttcca gcgccgccgt cagcttcata aaccgctgct
    41881 cggtgaggac gcctttggcc ttatcggtgt acaggctcaa aaacatcccg tcaatttcct
    41941 gattccggtc ttccagccgt ttccgttcct tctctgtctg ggaagcgtcc accagatacc
    42001 ggcgctccat ccggctgctg agccgctggt agaacgcatc ggcgtccttc atggcctgcg
    42061 ccgccaattc ctgtatatcc ttcagcacca gattgtacaa atcccttgcc tcaatcttgt
    42121 ggctggtaca ggcgttcttg cccagccggt tgtaagtctg gcagatatag tatgccctgt
    42181 caatcggctc ccgcatttcg ccggtgaagc ggttcttccc ggttctgccg accttttcat
    42241 accgcacctg catggacttc ccgcaggtgg cgcagtagac aagcccgtgg aacaggttat
    42301 agaaggggca ggcgttgcct ttcataatgg ttggcctgcg gtcaatgatg tcctgtacct
    42361 gttcccattc ctccggcgtc acaatcgctt cgtggcagcc ctcgatgacc tcccaatcct
    42421 cacgggggat aatgtcataa gtgttggagc ggatgccttt ctggtgtgtc cggcagacaa
    42481 gatgtgcgcc tttataaaag ggatttctca ggatatggct gatccttgcg ctcccccatg
    42541 aatagtagtt cacgtcacat tctgtattgc tttttactct ggtgatgggg attttatcat
    42601 ccatgagctg ctttgcgatc cgcatacagc cccagccgtc cagtgccata tcatagattt
    42661 ttcggatcac cggcgcagtc tccgggtcaa ggatcagatg ccctttgtcc tccgggtcac
    42721 gcatcaggcc aagaggcggc tgcccgccgc agaactttcc ctgccgggaa cgggtcatgc
    42781 gtcccgccag cacctttttg gaaatatccc ggctgtacat atcgttcagg atattcttaa
    42841 agggcgtgat gtccatttcc tgacgggtca ggctgtccac accgtccgtg acggcgatat
    42901 agcgtacatt gtgtttgggg aaaaagattt cgatatagct gccagcttcg atataattcc
    42961 taccgagcct cgataggtct ttcgtgatga cgcagccgat tttccccgcc tcaatgtcag
    43021 cgatcatgcg ctggaacgag gggcggttga aattgcgacc cgtatagccg tcatccacat
    43081 aatactcata ctggaacatc ccatgctttt ctgcgaagtc ccggagcatg agtttctggt
    43141 tgctgatgct catgctctca ttgtcgccgc cgtcatccag agaaatacgg cagtagagag
    43201 cggtgatttt ttcatagact tgttttttcc tgctcatagc gttctccttc tatgaacagg
    43261 gacacgatac cattatagta ccatgtcccc gtccctgaat caaccgtttt tacgcagctt
    43321 tttctgccat ttcccgctga tgacgccatt cctcgaagtg ttcgcaggtc tggcgctcaa
    43381 tgagctgctc aaaggcttcc cgcatggact tctccccgga gaactcccgc accaccacaa
    43441 aacggacttt attcttctga tttttgcttt tttccatagg ctacctccat agagagacag
    43501 gccgaacggc agcaccggca acgaagtccg gcaacgaacc tctaaaaacc tgtaaactac
    43561 cgtctggcct gcaaatctta ctttgtgaat tttcttgaaa aatctcgttg ctttcgttgc
    43621 cggagataat gataaatatt aaaatatccc ttatttactg gctttttccg tatccagaca
    43681 gggaaatccc cggcaacgaa cccggcaacc aagtggcaac catcccggca acagaccgcc
    43741 attttcaggc gatccactcc ttcggaagct ccaactggcg gcattcttcc tcgctcagtt
    43801 ccacaaatcc atcttcgttg ccgggcagtt cgttgccgga aacgtcccgt tcccagccct
    43861 tctgcctgcc gtatccggcg aacatccggg gattggagaa tggcttccag cccgtcacca
    43921 ctgtattcat gatctcgttg atattgtgaa gctgccaccg cttcggctcg tcataggtgt
    43981 ggcccaatgc ctccttaaaa agctgtttgg aacagaccat gctgccggta taatgttcca
    44041 gaaagccctg tatttgaccg gcctccgtgt cctccggcat gaaatccttc tgcacctcca
    44101 caagctgccg ctggatggac ttgctgaatt tcatggaata cttcccgctg cggtagatgg
    44161 tcatggcctc cgcccagacc tgcaacaggt aggctctgga agcgtcctcg tcctccaaaa
    44221 tgtgaacctc cgctttctcc gggtaaatca tgatgggaag gaaacgccgg ttcccggccc
    44281 ggtcaagggg caggaagtcc agcgtgttgg atgaaccgcc gaacacgcac tgccgcagcc
    44341 ggtctttggg ctgggcctca tagggtgtgc ggtaggtttc cttctggcga ctgatgaacg
    44401 agcggatttc ctctatgctt ttggcgctgc tggtggccag catctctgac atctcaatga
    44461 tccagtggcc ttgcagcttc aaaaataccc ggtcatcgtc cagctttttc aggtcatcgg
    44521 agaaccactc gtcccggatc gccagcaggc ggaagaagct ggacttgcca gccccctgtc
    44581 cgcccaccag acagagcatt tcctcgtatt tggagccggg agaaaacacc cgccggatgg
    44641 ctcccagcag gaagtgtttt aacatttcct ctacataatc gctgacttct gcacctaaga
    44701 agtgatggag acaggagcgg atgcggggcg tcccgtccca cacaaggccg ttcagcacgt
    44761 cctgtatggg atggtagcag ttctcattcg ccacgatgga gagcgccgcc gtcattttct
    44821 tctcgctggt caggccgtag ttctgctcaa aatagagaag cagatacttc acatccgtat
    44881 ccgtcagggc gctggtgttc ctgcgccagc ccaaatcccg cacgatgtcc acccggtcag
    44941 tcaggaggtt cagccggatg gcgtcccgca ggagagggtc atgggagaac accgtccggc
    45001 agttgccgat ggtgttggcg ggctggccct tctccgtgac agaaaggcct tcccgcacct
    45061 cgtcaacgct ctgctccggc gctaaagctt cggctgcgct catgacggcc tgccgggtgg
    45121 ctggcggtaa actctgatat tcgctgctca atcttcctca cctcttttcc atactcagcg
    45181 accacgcagg cccgttcctc catactgccg gacagcagaa tatccagcag atactccgtg
    45241 tacggctttt tttgcagggc ctccacaaac agcgggttcc atgcttcctc cggcgtctgc
    45301 ggggcatatt ccttctccca gcgttccagc agatgcagat aatcgcacaa cacccggaaa
    45361 catttctgtt ccgcctgccg gtagcgcagt tcctcgctga tttttcgctt gcccggcttc
    45421 ctgcggggcg ggtcatggcc tttagcgtca aagccgaccg agaaatcctc cgccagcttc
    45481 aaggccgcat ctttcttgtt cagcccgaac agacgggcgg taaaatcaat cacatccccg
    45541 tccgcctgac agccgaagca gtggaaccgg cggtcaacct tcatgctggg atgtttgtca
    45601 tcatggaagg gacagcaggt catcccgttc ctgccgagct gtatcccata agattccgca
    45661 gcctgccttg tggggacagc ctgcttcacc gcttcaaaca cattcgattt tctcacctcc
    45721 agacagagaa aaaggcatcc ggttttcaca gaaaatcaga tgccttacaa ctctatatcg
    45781 ttctttttgg tcggggcgat tcgcccctca cgttcccaac gctcccggtt atcccggtca
    45841 gcattaattt ttgcctttgc cagaatatcc ataatggatt ctttggcttt cttcttgacc
    45901 tgctccttcc cggcttgctt gacttccggc tgcatcagcg tccgctggat tttcgccaga
    45961 gcgtgctgca tggcgttttt gattttggta atgaccccat ccagacgctc agcggcatac
    46021 tcacgttcct tctttggagc tttgcgctcc ggcgaaagca cccatttttt cgattcctcc
    46081 accagacgaa tatcttcctt gtgcgtctcc tgccggacgg tatcggtcac cacctccacc
    46141 gccttgtcat aggccgcatc ggaaacatca tctaacagtg tctccacgtc ctcaattttg
    46201 agcgtcagtt cttccagctt ctgctcctgc gccgccagtt gctccttctg tttcatcagg
    46261 atataatcct gtttttccag atagtcccgc ccgccgtagg acggctcctg atccagatgt
    46321 agcccatgcc gtctggcgat gtcaaacaga atcgtccggc aaaccgcatc aaaggtctgc
    46381 ttgcggttgt tgttccggcc tttgggcttc tcagggtttg ggagagggat gcccagttct
    46441 tccagcgcct tttcctgctg gggacacagt tccccgtagc ggttctcaca gtcaaagacg
    46501 tgccgctcat ggatgtgggg cgtcccctcg tccagatgga gcgcccagtc caggatgtgg
    46561 atatgggaac cgaagcggcg ctcaaattcc tcataaaact cattgacgat ggaaaaaagc
    46621 gtgtccggcg ggacggattc tcccatcgtg ccgatctggt agatgctttc ctccgggcag
    46681 gtcttgttgt ttttcagcaa gtcctccacg gtgcggttgc gctccgtgtg ccgggttttc
    46741 tcattccggg cgttctgggc ctccacatgg ccgccgtaat gctcgttgta gtacatccgc
    46801 tcgatttcct caaagctgaa atccggctgc tccgggttct cccggaactc gtgtgtcgta
    46861 aagccccggt agcagtccca gtagacattt tgccttgccc gttctgcgtc aatgtgttcg
    46921 ctgttctcca catcgaaccg gcggtcattg tggcggggat tataggtgcc atgcttgccg
    46981 gagcgcccat tgtgtcgtgt cagtttcaaa cagatttcct ccttcctgcg aagatgttct
    47041 ggggccgggg aaggggcggc gaagccgcaa atcctgcgaa ttttgcgtca ggtaataccc
    47101 agtacgagtt gacgcaggca tcaactctcc ctgggcaagg cgtttcaccc ttgacccgat
    47161 aatggctgcc gcccttaacc tgccaatggg cgttgcccct tgaccccagc agtgcgctgc
    47221 cgcccctgca ccccggcaca aagggctggc tgccctctgt actcccgcac cggcgaagct
    47281 ggattccacc gcagaagcgg tgtttgccag tttcaccggc tccgtatccg cacatttgcg
    47341 cccataagcg gggaatggat acggaatacg cctgcgggga ttgccggtca aaacagcggg
    47401 tgcagacccg gcattttttc cgtaaacgcc ccggtttcag gcgatttttt cttccgcccc
    47461 taccacctga aagccatgct cttttgcgta ggcgctggcg gcggctttcc gttcctcgct
    47521 gtacggcggc accagccgga tcgacagccg ggacttatcc agcacatagg tcacgctgcc
    47581 ctccggcgtg ctgcgctcca accggcacag cagcgggtac ttccggctga aatcagccag
    47641 cctgcgcttc aagctggcgt tgaaggtgta gacgctggca aggttttccg cctcgttcca
    47701 gttgataatg gtttctttct catacttaga cagcttcctc atacagatcc tcctcataat
    47761 cagtgtaaga tttcagcaca cgcaggctgc gcttgaggcg gcggtactcg tccatctcca
    47821 tccgcagatg gtggtaaaaa tcctcgtacc agttttcgcc cacctccgtc tccaccttcc
    47881 gggccagccc cagcatccgg cgtttggcct ccgggtcaac cgtcagtgcc gtcagccatt
    47941 tcagccgtgt caccgtattg tggtggctgg gacagccgta agcgtagaga atcttctttt
    48001 ctttcatact cagtgtcata atgatttcct ccgtttcttt gctgcggagc catgcgcccc
    48061 gcatgattga taaacttgtt tctgtctgcc ggtctgcctg caagccgctc ctgcccggaa
    48121 tttctcccgg cgtatcggcc tctttggtgc gctcgatttc tgtcctggcg tcacttcccc
    48181 ggaggccata ccccttgcag ccggtcattc gattttcaag gttctgtgcc tgtctgcaag
    48241 aaccagttta ccgaaaaaag aggccctttc ccgtatatcc aaaacgtcgg aaaaacgctt
    48301 gaaatccgca tatttccacg cttatggaat cgtgctataa tgggtgtaat cactgaaaaa
    48361 cgataacgag agagggggct ttggatgtac gcaaagctga caatcccgga gcggctcaaa
    48421 gacctgcggg tggtagataa gcacctgacg ctggaacaac tggcggagca gaccggcctg
    48481 tctaaatccg cgctgggaaa atacgaaacc gatgactaca aggacatcag cccgtttgcg
    48541 attgcaacgc tggcggactt ttacggcgtg tccaccgact atctgatggg gctgacggaa
    48601 aataaaaatc acccaaacat agaacttcaa tctctgcatc tgagtgatga tatggtggaa
    48661 cttttgagca gcggcaaaat caacaaccgg cttctctgcg aactggctac ccacccgaat
    48721 ttccggcggc tgatgataga tatggaaatc tgcatcgacc agatcgccaa tatgcgggtg
    48781 gagcagatga atctggtgtt ggaggccacc cggcagaccg tcatgaacaa gtatgcaccc
    48841 ggagaagatg acctctatat gcggacgctg gaactggggc aggtgcagga aagcgatttt
    48901 tacagccacg tcatgcacga tgatctggac agcatcgtcc gggacatccg ggaagcacac
    48961 cgcaaagaca agaccaccgc cgacccgcag cccacgctgg atgatgtgaa ggaaaaattt
    49021 gagcaggcca tagggcaggg cagcgataca gaaatgctga tccacgagtt ctgcgataag
    49081 ttgcagatac cctttgagaa gatttcctcg gaggacttct cggcgttcct gcggatactg
    49141 agcctgtcca agcagctcaa aagctccaac aatatgaggg gaaaggccag ccccattcct
    49201 cccaaccgca aggccagacg taagcggaag taaaatttcc atatccggaa gcggcaaaaa
    49261 agccatccgc cgattgattg gcaaatggct ttcgtgaagc tgtattctgt tgtagtggtt
    49321 gtgcccagtt tgggaacagt cactattgaa gatgctattc tttgtctttc tgcgatacct
    49381 gtgcatgggc ttcctgctga acaagcagcc ccgcagaaaa gccatatttg aagtggcttt
    49441 cgttttcctc acactgggcg attgccacct gctcccgcag ttcttcaacc agtgcatagt
    49501 cctctttgct cagacgctcc gataaagtgt ccatcagggc ggcgctggct ttgactgcct
    49561 gcttgaacct cggtgaatca gcgaccaccg tttcacaggg ataaaatctg ccgttataca
    49621 tttcttccag aatcatgcac ccacctccct cagacaaaaa tcatctcaga caggatgatt
    49681 tcctctaccc ggctgcggat gctgttcatg cccccgaccc atgcaagctg gtcacgggct
    49741 ttcagttcct cggtgacgcc ctccgcagcc tgcatctgcg agatgataca cgccagtcgg
    49801 tcatcggcct gctcgttcag atcggcaaga tacgtccaga gttttcccga cagaagcagt
    49861 tcattgtact ggccgggacg gtgttcttgc agaaacgcct tgtgtatccg accccaccgc
    49921 ccgatggggc ggatttcctc cgacaacttt agatcgggga tatagtagtc tcccaccagc
    49981 gtatagctga tgccgttgtg atggattttc tttggtaaat gctccatgtt cgacctcctg
    50041 tattcagttt tttcagaatc cagttgatcg tgtccctgtt catgtgaaat tcaaggcaac
    50101 tgaactcttc acgaacaagt atggcatatc cttaccagtc ttttgatagt ttaaaccaaa
    50161 gctcttttga tttgaccttg tttccgacgt gttatacagg tcgatagttt cttttcccag
    50221 tgtttcagac aattctttta gcgtagtttt ctccttgcca ccaagaaaca aggtactatc
    50281 acagttacct acgattgtat cagcgttatc cttgtagatt gcctttagct gagattgtgc
    50341 ttgaagtatg atacttgctg aaatctctct tgaacggatt gttgcaatta acttctcaaa
    50401 cttaggaatt aagccgatat tagcgaactc atcaagtaga cacctcacat ggacaggtaa
    50461 tcgtccgcca tatacatcat cggctttatc acataaaaga ttaaataact gtgagtacat
    50521 aatggatact acaaagttaa atgtatcatc ggtatcggag ataataacaa atagtgctgt
    50581 ttttctatct cccagtgtat caagctcaag ttcatcgtca ctcatcaagt ccctaagttc
    50641 ctgaatatca aagggagcaa gtcttgcacc acaagaaata agaatcgatt tggcagtttt
    50701 tcccgcagcc aatttgtact ttttatattg cttcactgca aagtgtgtag gttctttctt
    50761 ctccagtgct tcaaagagcc tatctatcgg attcatatag gtttcattat cttctctgac
    50821 ttctgaagca tcaatcatat caagcagagt tgcaaagttc ttttcttctc ttggagcttc
    50881 atagaagata tagccaataa gtgctgtata gtagagtttc tcagctttca cccaaaaatc
    50941 ttcacctgat ttttctccct ctcccttggt gtttgcgata attgtctgta ctaatttgag
    51001 tatgtccttt tcacttctaa gataagcaaa gggattgtat ttcatagact ttttgaagtt
    51061 aatggtattt aggattttta tctcatatcc gttatcttca agcatcttac cgcactcaag
    51121 gactatagtt cctttaggat cggtcacaca atatgacgag tgcatctgca taaggttcgg
    51181 ttttacataa aatctcgttt ttccacttcc tgatccaccg attacaagta cattcttatt
    51241 tcttgcatac tttggatttg ccggtctgcc attcatggtt aatcgttctg tttgcgtaag
    51301 taggatattg ttttgaaact tttcatccat atatggctct atatcttttt tcgttcccca
    51361 tcttgctgag ccatactctt tcccctgtct aaacttttta gcatttttcc ctttggtata
    51421 tacgataatt ttgattaaaa cagctactac cactcccatt aaaatatccg taggatgaat
    51481 gcttggaaga aagctcatgg tgttaagttc cagtatacct tgaaatattt tgtctattac
    51541 atcgccacca gtatatgctc ttacatgatg agaaaagata ttgccaacat agaaaaatgc
    51601 aagataggga atgttctgct ttagaaactt tgccttatct tgcaccttaa ataagccttt
    51661 gatgtctttt agtatcttat ctatcatagg ctttgctcct tttgtttatt tttgattttc
    51721 tcttttgaaa tagagttttt agccatctct ttgaacttct caatattctt gtgaatagat
    51781 tctttccttt ctgctttctt ttctgatttt gaaagagcgt gcttaaaagc tttatccatc
    51841 actttcatat ctttggcttg aaagaataca gagtttttac ctgtctcttt atttttcatc
    51901 actgaaaatt taacaccgta tctattcaac tcttttttaa gttctttaag ctcagcttct
    51961 tccactggga tttcttcaag ctgtcccttt ttaaccatat cttttagctt tacttcattt
    52021 ccgttggcat tgaccagttt ttcaagtcct ccgaatttct ccgcttcttt catcaatttt
    52081 tttaataagt ttaatagaac ttttccagta actttaccag catttatctc catatttaag
    52141 gtctttcttg ctatttcttc attgatcaca tcaatatcct cctctcatat catagcttac
    52201 aagagccgaa taataggcat ctattccact tggtgcatta taagcggcgg tcaggacaaa
    52261 agctcttata tttctaatcc tatattcatt ttctcttaat actcctacta agtactctaa
    52321 atgtcctgaa ttaagtttcc taaatctttc ctttactact tgtacagata tctttgtttg
    52381 gttgatagta atgaaagatt catcatctaa taccattaca tctgccagta tctttaatat
    52441 ttcatcaaac tcttcagcca cactcttatt cccaatactc atatgttcat aatatcctgt
    52501 ttggtgtcgc aattcttcta aacaattttg atatatattt ttatagctta tatctatcct
    52561 ccctcccttc atattctctg aaaataattg attcttttct ttgatggaag gatggatgga
    52621 aatatatgct gaattatttg tagtattatt tgtagtatta tttgttgtat tctctggtaa
    52681 tggttggtgc aaattatcct tttgcattag tgcattttga acctgtgcat tagtgcaatc
    52741 tgcattattg cattggctga ttttgccctt atccattggt gcattttgcc ctaatgcatt
    52801 gggcatagaa ttttttaatg cttctgtttg tattttttgt tttttcaaat ccatatccat
    52861 atataattct tcaagtttat cattattaat tgtataccat tttgttttat ctcttggatc
    52921 cttattgtaa tttccaacta aaagataacc tgctttttct aactttgaaa aagttctttt
    52981 tactgtatca aagctcatat agtcaaagtc attttcttgc catgctcgaa ttgaattata
    53041 tgtccagtat ttaccatcat gataattctt tcctgatttt ttatttattt ctatccaata
    53101 attaatctgc tgtagtatga gtgcttcgtt taatcctatt tctctagcaa gagttttatt
    53161 cgcaactata ggttgttcat caaataaata cataactcat caaacactcc tttctttttt
    53221 tcaaacaaaa agacgatagt tatttctatc gtctaaaatc atatataaat atcattttct
    53281 aaatagagcg tattgcttct ttcactcccg ttaaaacggc tataacagct gttccaacca
    53341 ttggtattcc gtatgggctt aacaaaaatc ctaatatcaa tgcttctatc cctatggtaa
    53401 cctctttttg taaaaaagat gctattgctc caaatatgca aaacatcatc aacaggtaaa
    53461 gtattgctgt tcctatgcca agtaaaaatg aaagaaatgc aatgagaata cttaacgcta
    53521 tactaattgg aaataagata atttttaata tccatctcat atatactctc catttatact
    53581 aaatcatttt aaaatgtctt aaagcgtcca ttatagcttc ttctttttca ggcgtacact
    53641 gtgggatagc ctgattgtct tttttagatt tattataatg ctctctcagt tcaattccac
    53701 atttcttctt aatctgtgca atataaagtg tagaaacctt tagcccaaat ttttctaata
    53761 catactcctt aatttgagca tatgttgctt tgctctctgc acttgtcaaa tcaagctcat
    53821 ccagcttaat ttcaacatct atatgcttat cgacatcaag tttggacaat aacgctaccg
    53881 tctccacatg tgtagaatga gggaacatat ctacaggttg tgcctcaaca catttaaatc
    53941 cgtgaacatt tagatagtct aaatcccttg ctaaagtaga aggattacaa gaaacataga
    54001 caattctttc cggattcatt gaaacaatag tctctaatac cttttcgtca catccctttc
    54061 taggtgggtc tacaactact acattggcag tcttaccctc tctatacatc ttaggtacaa
    54121 tttcctctgc ctttccaaca taaaattcag cattatgtat attgtttaat ttagcattta
    54181 tcttagcatc ttttatggca tcatctataa tttctattcc ataaaccttt ttagcctttt
    54241 gtgccaaaaa taaagatatt gtacctatcc cacagtaaat atcaaaaaca gtatcagtat
    54301 catttaaatt tgcatattca agagccttat catacaatac tttagtttgg acagggttta
    54361 cttggaaaaa tgataatggt gaaatctcaa acactaagtc acctatataa tcgtttattt
    54421 ttccatctcc atatataact atattttctc ttcctagaat agcattcgta tctttttgat
    54481 ttacatttac aaccaaagtc ttaaatccag gtatattttc cttaagcact gaagctaatt
    54541 ctttaagata tggaagtttg tttccatttg ctactaatac aaccattact tctttagttg
    54601 taaaacctat ttttgttact aaatgcctaa tcaaccccgt gtgagtcttt tcatcatata
    54661 tacttacctt ataggccctt atatatgttt ttattatttt aacaatttta tcatttatct
    54721 catgttgtat tacgcatttt tctgtcgata ttacatcatg agtcttcttt ttatagaagc
    54781 caataattgg aataccatta ttcttttgta ttggaaattg tgctttattt ctatatctat
    54841 atggattatc cattcctata gtgtcatgaa cttctacatc ctctaactta cctattctcg
    54901 aaagtacttg tttaacttca tttgttttca tttcaagttg tttattataa tctaaatctt
    54961 gtatctgaca ccctccacaa tccctaagat tgtcactaca cactcttttc accctgaatg
    55021 gagacttttc tattatctca acaatatctc cctcagcata atttttcttt actttattaa
    55081 tcctaacttt aactttatca tgtattaatc cgccctctac aaaaactgta ataccattgt
    55141 actttcctac tcctactccg ccttgaccaa tgtctactat atctacaata tacatctcgt
    55201 ttttcgaaag cataaatcac cctccaatca atctattata tttaaatctt tacaaacttt
    55261 tttctttaaa tttatattaa aatattgcta ttaattaaac tatatttttt actaagctac
    55321 aatattttat tttaccaaaa aaaaaggagc ttataaagcc ccttagtaaa taaatttatt
    55381 atttttctta taaaactcta gttgaacctc tatataccac gcctcttgat gcatccacag
    55441 ttacaacttc tccatctttt actagatttg ttatatcagt tgcagacaca ataactggta
    55501 tatctaaatt tatacctaca atagctgtat gtgatgtcat tccaccattc tcagttacta
    55561 cagctcctgc tttttctata taattgttca tttcagcatc agtagcagta gaaacaagta
    55621 tgtcgccttc attaaagtct acacaagcat ttccattctt tattatacga actttacctt
    55681 ctaccgtttg agtacctaca cctatacctt gcacgatttc ttcgcttatt acatgaacct
    55741 ttattaaatt agtagttcca cttactccta ctggtactcc agcagttata actactaatt
    55801 ccccattatc tatataatca gcttgtctag ctgcctcaat tgccttttct ataacttcat
    55861 ctgtatttcc tgcaacagaa gacttaactg gatatactcc ccatgtaaga gctaatttat
    55921 tcatagtctt ttcattattt gtagtagcta ttattggaga ttttggtcta aatttagata
    55981 ccattctagc agtatatcct gaagaagtag atgttattat agctgatgca tttaaatcta
    56041 ctgctgttgt acaagttgca tggcttattg catctgttac agtaacattg ttagtaccat
    56101 tttcctttag taatctatca taatctaaag tctcttctgt tctttttgct attgtagcca
    56161 tcatttttac tgcctctaca ggatattttc cagcagcagt ttctccagat aacattatcg
    56221 catctgttcc atcatatata gcattggcaa cgtctgtaac ttctgctctt gttggtcttg
    56281 gatttcttat catagaatca agcatttgag tggcagtaac aactggcttt gctaattcat
    56341 tacatttttt tatcatcatt ttttgaacta ttggcatctc ttcagttggt atctcaacac
    56401 ctaagtcacc tctagccacc attataccat cagaaacttt taatatttca tctaggttct
    56461 ctacaccttc ttggttttct attttagata ttatttgtat atcagttgca ttattgttct
    56521 ctaatacttc tcttattgct agtacatcag atgcctttct tacaaaagaa gcagctatat
    56581 aatctattcc ttgacttatt ccaaactcta tatcacttat atctttagga gttattgctg
    56641 gtaaatttat cttaacacca ggtacattta ctcctttatg atttttaact attcctgaat
    56701 tttcaacaac acaaactata tcttctccat ttatttcttt tactcttaag ccaactaaac
    56761 catcatctat tagtatagaa tctcctgatt taacgtcttc agcaagacct ttatagctta
    56821 ctgtacacat ttctttagtc cccataatat ctttcatagt tatagtgaac ttttgccctt
    56881 cttctaaaaa tacctctggg tcttcaaagt tacctgttct aatttctgga cctttagtat
    56941 ctaataatat agcaactggc ttatttaatt tttctctaac tttctttgcc atatcaattc
    57001 tttctttatg ttcttcatgc gaaccgtgag aaaagttaaa tctacacaca tttagaccat
    57061 tttgcataag ttgagttaaa atttcctcac tttgtgatgc tggacctagt gtacaaacaa
    57121 tttttgtctt ctttacatta tttaacatat ttgctcctcc tgagatatta ttccacagat
    57181 ttatttttaa tactaaaact tatatagata atatttttgc catctcataa gcttctttat
    57241 caaatacttt cttttgagct aaagcttcac ttatttctaa atcaattatt ttgttttctt
    57301 ttattcctac aactctagct gattttccat ctaataataa ttctactgct cttactccaa
    57361 gtctacttgc tagtattcta tcagatacag ttgggcttcc tcctctttgg acatgcccca
    57421 atacagtgac tcttaaatct gctcctgact cttttttaag ttccttttct aaatcactag
    57481 cacttccgac tccttctgct aaaacgatta tactatgtct cttacctctt ttttgagtag
    57541 tctttaatct tagagcaact tcatctactg taatttctac ttctggtact attatagttt
    57601 ccgctcctcc tgcaagacct gcatataaag ctaaatctcc acagtgtctt cccataactt
    57661 ctactatgtt aactctttca tgagatgatg atgtatctct tatcttacct atagcatcta
    57721 taatagtatt cattgcagta tcaaatccta tagtataatc tgtatatgct aaatcgttat
    57781 ctatagttcc tggtatgcct atagctggga atcctaagtc actaagtttt tgggctcctg
    57841 caaatgaacc atctccacca attacaacta agcaatcaat tttatacttt ttaagaattt
    57901 ttactgcttt taatctacct tcttctgttt taaattcttc acatctggaa gattttaata
    57961 tagttcctcc cctgtgtata atatctccaa cagaagataa gttcatttca gttaaatctt
    58021 cttctagtaa tcccttatat cctctattga ttccatatac tttgcatcca taatatattg
    58081 ctgatcttac aaccgctcta atcgcagcat tcattcctgg agcatctcct ccacttgtta
    58141 gtaatcctat agttttcatt ttttacctcc ttggatttta tgtcaatttt ttattataat
    58201 cattgttatt gtattctaat ggactttttt tgtccctata tcacttttta atcccatcaa
    58261 gactaaaaaa attaaatgtc cttaaatgtc cattttgtga tattatatca caaaacggac
    58321 aatatttttc atattattat aatatattta gatttttata taaaaaatca agcttttact
    58381 tttatgttat ctttaggaag taactcttct aagttattta taaattcatc acatatacaa
    58441 gtcaatgccc cggtcatttt atatgcttta cgtttaccat ttatattttc atccataggg
    58501 tacaagtata tactgtcatt acctggatat tgtttggcta tttctacaat attattagtt
    58561 gtatcagtat cagacatact atctattttc aaatacaatt ttttaccact ttctggctta
    58621 atattttcta catttctagt tcttcctgaa ttgtagtttc tatctgcaaa gtcagtatca
    58681 tcatttatat cttttatctc tctagctata agttttgcat tttcgccttc ttttatactt
    58741 aatgttcctt ttacatatat tatattatct tcattcaaaa tgacattata cttttgtaat
    58801 aattgaggaa aaactattat ttctatagca ccatacaaat cttctaattc aataaatgcc
    58861 ataatttcat ttcgttttgt tgttttaata gttttagtta caatcattcc acccattata
    58921 acatctgttt cattcatttg taggtatgac tcttcttcct cttttaatga atttaatttt
    58981 ccattatcca tagatgtttt ttcttgtagt tctttttcaa attgtgataa aggatgtcca
    59041 cttacataca ttcccaagac ttctttttca aggttaagtc tctctctttc ttcatactct
    59101 tgccttttag gtaaactgta tatttgttga aaatcactat cttcctcact atctccaaaa
    59161 gcatcaaata aagaaatttg accttgaaca tttttctttc tatccataga tacactttct
    59221 aataaacttt cataaccagc catcagacta gctctatttt cacttatatt atcaaaagct
    59281 ccacatttta ttagactttc tattactctc ttatttgtat ctttttggtc taatcttttg
    59341 actaaatcta ctaaatctac aaataatcca ttagattctc tttcagcgat tatattgttt
    59401 attatattta caccaacatt ttttacagct gctagaccaa atcttatatt gtcgccttca
    59461 actgaaaact ttgaaaaact tttgtttata tctggaggta atacatctat ttctagtgct
    59521 ttacattctc ttatatactc aactacctta tcagtatttc ccattacact ggttatcaat
    59581 gctgccataa attcaactgg ataatatgtt tttagataag ctgtctcata tgctaaaacc
    59641 ccatatgcag ctgcatgtga tttattaaat gcatatctag cgaagtctat catatcatca
    59701 aatattttat tagctatttc ctcttctaca ccatttctga tacaccctgc aatttctata
    59761 ttaccatctt catcaaattt accatgaata aaatattgtc tttcttcttc catcacgtcc
    59821 atctttttct tactcatggc tcttctaact aggtcacttc gtccgtaact ataaccacct
    59881 aaatctctaa cgacctgcat aacctgctct tgatatacca aacaaccata tgtaacttcc
    59941 attataggct ttaatttttc atgtagatat gttacatctt ttgggttgct tttatttttt
    60001 atatatgcag gtatagaatc cattggacct ggtctaaata gggatattcc cgctacaata
    60061 tcttcaaaat tgtctggttt tagctgtttc ataaaacttc tcatgccagc actctccaat
    60121 tgaaacaccc ctagtgtatt tcctgatgaa agcatttcat atactttcgg gtcgtcataa
    60181 tccatctttg aaaagtctat tttttctgtg tagtttttta taactctatt tttttctatt
    60241 aaatcaagag tatctcttat aactgtaaga gttcttagcc caagaaaatc catctttaaa
    60301 agcccaagtt cttcaagtgt tgtcatagta aactgtgttg tgattgcatc ttgatgtttg
    60361 tacaaaggta catattcatc tacgggattt tttgatataa caactcctgc tgcatgagta
    60421 gaagcatgtc ttagcattcc ctctatctgt tttgatatat ttattatttc ttttgtttcc
    60481 gggtcttggt catacaattc tttaagatta ggatttgtat ctagggcctt atctattgtc
    60541 attcctaaag caaatggtat ttccttggct accttgtcaa ctttattgta gcctatatcc
    60601 aatacccttc caacatctct tattgcagct tttgcaccca ttgttccaaa agttattatc
    60661 tgggctacat ggtcatcacc atattttctc tttacataat ctataacttc ttctcttctc
    60721 tcataacaaa aatctatatc tatatctggc atggatatac gttctggatt taaaaaacgc
    60781 tcaaataata gagagtactt tattgggtct atatcagtta tcttaagtgt atatgctact
    60841 atcgaaccag ccgcactacc tctacctggt cctactataa tattattttc tttagaaaaa
    60901 tttataaaat cccaaacaat taaaaaatac tctacataac ccattttttc aattacatct
    60961 aattcataat ttaatctctc tagtatctca ttgctaggat tatcatatct ctcttttaat
    61021 ccattaaaac acaactccct taaataagtt tctggtgtat atccttctgg aacatcatac
    61081 ttaggaagat gtatagtatt aaaatcaaac tccacattac acatatcagc tattttcaca
    61141 gtattgtcaa tagcctctaa tgcgtatgga aataattctt ccatttcttc tcttgatttt
    61201 aagtaaaact catcactacc aaatctcatt ctatttgggt catttaatgt ttttcccatt
    61261 tgaatacaca taagaacatc atgtattttc gaatcttctt tatttacata gtgaacatca
    61321 ttagtagcaa ctagtggtat accagtctct tttgataatt ttactaaacc agagtttact
    61381 tctttttgct caggtaaatt gtgatcttga atctccaaaa agaaattttc ttctccaaat
    61441 atgtctctgt agtcaagagc aaattgtttt gctttttcat aatttctatt cattaaagca
    61501 cgtgctacat ctcctgcaag gcatgcagaa agtgctataa tccctttgtt atgtttttta
    61561 agttcttcca tgtcaactct aggcttatag taaaaacctt ctacgtatga agttgaaact
    61621 atttttatta aatttttata tccttccata ttcttagcta acagaactaa atgtccttga
    61681 tatttgtcat agtttgggtc tttatctctc aatccacgtg cagctgtata gacttcgcac
    61741 cctataattg gctttattcc ttcttttata gctgttttgt agaaatctat aacaccaaac
    61801 atacaaccat ggtcagttat agctattgag ctcatgttta gttccttagc cctttttata
    61861 agttttttca ccctcgaaaa cccatcaagt aaactatact cagtatgtac atgaagatga
    61921 gcaaaatctt tgttcattaa atcacctcct gctttataat tttaatttta ccacaaatat
    61981 atgtcctaaa acaaaaacag agacttttat ctcaaaatag gaattaactt caacatatac
    62041 cattatatgt tataaattaa tgcttattct gagattgtcc cctcaattta ttgctatgat
    62101 ttttttttca aatctgttgc aatttcttct atttttctaa gtctatgatt gactccagat
    62161 tttcctattg gtggttctaa catctctcca agttctttga gagtcatatc ctcatactcg
    62221 attctaatct tagctatctc ttgtaaattt tctggcaaac tgctaattcc tatagtctct
    62281 tgtatgagct taatattttc tacttgtctt actgctgcat ttactgtttt agaaaggtta
    62341 gctgtctcac aatttactat cctgtttaca ttatttctca tttcttttac tattttagta
    62401 ttttgaagac ttaaaagagc gtggtgtcct cctataatac tcaacaaatc tgatatttgt
    62461 tcactctctt tgatataaac aacgtgatta ttttttctag ccacaatttt actattaaaa
    62521 cctaaagagt ttataagttc ttttagagaa tctgcaaact cctcattatt agttacaaat
    62581 tccatatgat agttcttttc tgggtcgctt atagaacctc caccaagaaa agctcctctt
    62641 atgaaagctc tcttacatcc ttcatgctta attaagttct ctgaaatttt gtttatagta
    62701 aaaaagcctt ctttatcttc aagtatacct aactttttta ataacatttc tgaacccatt
    62761 tcgcttgtta ccattaatac ataactgtta tttctcttta gcatttgatt cttatttact
    62821 gagattgtag tattgatatt aaaattcttt tttaataatt taaatacttt acgggctatt
    62881 gaatttaact ctgttgttat ctttagattt agcttcttat atccaacgat ttgtatactt
    62941 ccccctgatt taactagagc tgataattca gctatattac aacattcatt cccagatata
    63001 actcttgcta gttcattttt ggtttcagtt gaaaaagaca tatctatcac ctattaacta
    63061 atttttcttt aacctttttt ttatttcttg taaggcaacc tttattatct ccttttcatc
    63121 atcataccta gctaggtcta ccactctatc tagatggata aatttagcat caataaagcc
    63181 ttcctctttt tgtggaatag tatccatatt ctttgcttgc atcaaatacc aaaaaactgt
    63241 tttatgaact gctctatttt catcccagtt ttctttgaat gtatagtgta tttcacctaa
    63301 atatttcaat atatctgctt ttactccagt ttcttcactt acttcacgta atgctgcctc
    63361 ttggttattt tccccttctt ctacttttcc cttgggtaac acccaatctc cattgaactt
    63421 ccttagtaaa agaatcgtat tgccaaacag gactacacca ccagcactaa cctcttctct
    63481 catcgatgtt cactccttaa aactaacata atgataagga tttctacaca attttttaaa
    63541 atcctttttt actaactttt gttgtaatta tgatttaggg ctaactcaat aactatgtta
    63601 gatatatact ttgcatcatg tcttatataa ttatttttta tttcaattaa attcttctct
    63661 acggttttta tacccatctc ttttaattta tctttttgct ttttatctaa caagacttgc
    63721 tctgctccat cttgtttata taagtcaagc atttcttctg gcaatatctc attatttgcg
    63781 attacataat ctattaaatt ctcatcagtg tgctttacta tagcattaac atgctctaat
    63841 acattgtacc cttctgtctc acctggttga gtcattatat ttgctatata tacttttgga
    63901 gctacagacg atttaatagc gtctacaatc ccctctacaa gtaaattggg tattatactc
    63961 gtatataagc ttccagggcc cataattata atgtcagcat cgtaaataga tgcaataaca
    64021 tcctttaatg gtttgacatc ttttggattt aaaaaaattt tatcaataga gcttttttga
    64081 atttttgact cttcaggtat actagattct ccatttatta tattgccatt ttctaatttg
    64141 gcaactaaat ttacatcttc caaagttact ggtaaaactc ttcctgttat agcaaaaatt
    64201 tcacttaatt tatatacagc tttttcaaag tttccataca acccattcat agcagccaaa
    64261 aacaaatttc caaaactctg accttttaaa agcccttctg taaacctata ttgcataact
    64321 tcattcattg ttggctctat atttgcaagt gctaatagac agtttcttat gtctcctggt
    64381 gggagcattc ctaagtcttc tctcaataca cctgaacctc caccatcatc agcaacagtt
    64441 acaattgcag ttatattttg cgtagtgtgt ttaagacctc ttaaaaatac tgattgacca
    64501 gtacctccac ctatcacaac cacatttggt tctctatttt caacaataga cctttgaaat
    64561 agttttactt tttcattttt tgctttttta tatataaata acgatacaat taatgcaatc
    64621 caacctaaaa ttcctacaat aataagtaat ttagccatgt ttgcctccat tatcgcaaca
    64681 tagagtctct atgtttcttt acaactctat atcccttatt agataaatcc tccgctataa
    64741 gatttgttat agtaactgac ctatgtcttc cacctgtgca tccaacacca attactagat
    64801 gttgttttcc ttcatctata tattgaggta ctaaaaaatg aatcatatct aaaagtttca
    64861 cataaaattc ttcactgatt ttagaattca taacataatc tctaacttct ttatcatcac
    64921 cagttttagc tcttaactct tcaacataat agggatttgg aagaaatctt acatcaaata
    64981 ctaaatctgc atcagcaagt ataccatgtt taaatccaaa agatactaca gaaatagtta
    65041 ggtttggatt atcttcacca gaagaatata ttttctttat ttcttcctta aggtctttag
    65101 gtttcatgtt agttgtatca attatacatg tagataactc ctttaaaggt tccattatct
    65161 ttctttccat ttttatacct tctggaatct gcatatcttt tgccaatgga tgatttcttc
    65221 ttgtcatttt atatctcttt aacaatacat catcattaca gtccaaatat accatttcat
    65281 attcataatt ttctttttct aaataattta agctttcatg aagcgcttca aaaaactttc
    65341 tccctcttat atctatccct agagcaactt tatcaatact tgaatttggt tgataacata
    65401 attctgcaaa tttagttatc aaagcaggag gtagattatc tacacaataa aatcccatat
    65461 cctctaaagc tctcattgtc tcactttttc cagatcctga aagacctgta actataacaa
    65521 atttcattgc taactcctcc cttttatctt atgtttatta tattataatg ttttctacta
    65581 acgatatatc cagattagag tcagatatag tcttcatagc tagtttaaaa tcaccaactc
    65641 tttctggaca atgtgcatct gagcctatta taaatttatt ttctagccct tttattttat
    65701 ttaattgatt tacattcaaa aaagaatggc tactatttat ttcaagtcta gtatttgtct
    65761 tttttgctgt ctttgctact tcttctatat acacatcgcc tttatctcct ggatgagtaa
    65821 ttataaaaat atcattatta cttatcgcat ttattacagc ttttgtattg tattcttttg
    65881 ctttttcagt tttaaaaata tagttattac aatgatttaa taagcttctt aatgatgttg
    65941 gagtagaacc aaaatgatat ccagccataa tataatcaaa atctctaata aatttatcat
    66001 ccatatctat attgccttta tcatctaata tgttacactc catacctaaa agtatattta
    66061 tatcattgta cttatcattt aaataatcta cttcttctct cattttgaga atatcttttt
    66121 tctttacacc atatgcaata tgtttatagc tatggtcaga tattcctatt gtttttattc
    66181 ctttagaaat ggctaccttt acattatctt caatagtccc tttgccatga ctatataatg
    66241 tatgtgtatg ataatcagcc agtattttca tttgttaatc ctctccaagt attttgactt
    66301 cttcttccaa cataacccca aacttggcat atacagcact ttttataaca tatattaaat
    66361 ccaaaatatc tttagcactt gcttctcctt gattgactac aaatccacaa tgcttctcag
    66421 atacttgtgc tcctcttaaa gtaagacctc tcaatccagt ttcttctatt aatttacctg
    66481 caaaatatcc tgttggtctc ttaaatgtac ttcctgcact cgcaaaattt aatggctgtt
    66541 tagttattct tcttgtcgct aaatcatcca ttaaactttt tatttcatca taatttcctt
    66601 cttgtaattc tagttctgct gataaagcta tatatccgtt tttcgatatt atactttttc
    66661 tatacccaaa ttccatctgt tcatttgata attcaaatat attgccttcc atatccatca
    66721 gtcttactga cttcactatg tccttcattt caccaccata tgctccagca ttcatggcta
    66781 gtgctcctcc caatgtacca ggtattcctg atgcaaactc aaaccctttt aattcttgct
    66841 tttggagagc tttacctaaa actgataata aagctcctga ctgagcagtc atcctagtat
    66901 catttatctc ataagaattg aaattatcag ctaattctat taccactcct cttattccac
    66961 catctttaat taaaatgttt gaaccatttc caattattaa atacggaata gattcttctt
    67021 ttaccaactt aagtacattt tttaattgtt cttcagttct aggtctaact aatatatctg
    67081 ctggtcctcc taccctaaat gaaatatgtt ttttcatagg ttcatcgact tttatatcct
    67141 ctttacttaa tatatttaat aaatttatat atatatcttg attattcatt gtatttcctc
    67201 ccaaattata ctgaacttta gctgtcatta ttatttaaat tacattagta tatagtatta
    67261 actatattca ataatatatg attttgctta tcatatggat tttatatttt aagcttctta
    67321 attacaaact atattcaata aatatataat aaagtaaaaa aaaaaaaata acaacatata
    67381 aaagaatata aataatttaa aaaaagcatc aatatcactg atatcaatgc tttaaaaggc
    67441 aatttaattc gtattattta tctaactatt tttgacatag caaagataat aaatccctcc
    67501 aactactatg gaacctccaa cgatatttcc aagcgttaca aataccaaat tagttataaa
    67561 ttgaaccata gttatatttg ctcctaatgc cattcccatt ggtataaaaa acatgtttgc
    67621 aacactatgt tggaatccgc ataatacaaa taacattatt actgtccaac atgaagccaa
    67681 ttttgacatt atatcttgag atgctgtagc catccataca gctaaaacga ctaaaatatt
    67741 acaaagtatt gcttttaaaa acattgatgc tatagttgaa ttaaccttag cttctgccac
    67801 agctatagct tttgtagctg catctcctgt caatgtagaa gaataaaaca caagtgcaac
    67861 tagcagaatt gaacctacta aatttgcaaa ataaaccagt ccccagtttc ttaacatttt
    67921 tcctaatgtt atttttttgt ccataagtgc aatagccatt aaggtgtttc cagtgaataa
    67981 ttctgctcct gccaccacta caagcataag acctactgga aagaccatag cacttaaaaa
    68041 gctagctacc cctgggtcta tcttgccaaa agtttgacta actatcatac tccccaatgc
    68101 cccaaaacct ataaatgctc ctccaaataa acctaataga attgcatttt gtgtcgaaat
    68161 gtttgctttt ttaatgcctg cacttatagt tgcttgtgca gtttccccag gtgtcaagaa
    68221 tcctttactc ataaatcctt cccccttaaa aattatactt tcccttgtct ttttatggca
    68281 ataattatat aataacaata tgatatataa taaattttat taacataaaa aatatactat
    68341 atacttatat atacttgtct catataatta tactatttat tatataacaa ctttgttaat
    68401 ttcgaaataa tttatattga atttgaaaaa tttacctttt gattatttat tttacttttt
    68461 aattattgag gtattatata taatagatat atgtttgtgt tattatatat aattacttat
    68521 tttaataaat atttgaagga gggaatttta tgtttgatat atcatttttc gaaatgtcaa
    68581 tatatgaaat agcagttact acaatatata taattaattt catggttata ctaaacctta
    68641 tctttagaga aaaaagaaac ataaatacaa ctttaacatg gcttcttata ttagtattga
    68701 ttcctgctct tggattcata ctttatatag cttttggcag aaatatttca aaaaacaata
    68761 tgtttagatt gaaagaaaag gatgataaga taatcaaaag taatatacta gatactcagg
    68821 ttaaactcca atctacatct gaaattgatt cagatataca tcaacataag gatatgattt
    68881 atgcattggc taattcaaat aatgctcatt ataccaataa taacgatgtg tggatttatg
    68941 ctgaatctag tcaatttttt aacagtcttc ttgaagaact taaaaaagct aaaaaatata
    69001 taaacataca attttacatt ttcaaagatg acaaaatcgg aactgaaata atagacattc
    69061 ttatagacaa agctaaagaa ggtgttgaag taagactttt atttgatgca gttggtggta
    69121 gaacactaaa aaatagtaca ctttccagac ttaaagaaag tggtgttaaa gttggaagct
    69181 tcttcccatc attcttgaaa attgtaaact ttaacttaaa ctatagaaat caccgaaaaa
    69241 ttgttgtcat tgatggtaag gtaggatttg taggtggata taatgttggt gatgaatatt
    69301 taggtagaaa tccaaaattt ggtctatgga gagatacaca tactaagctt actggtgatt
    69361 gcgtaattga cctaaatatg agattcatcc ttgattggag atatacaact aaagaagatt
    69421 tagatttaga aaagtacttt atagaagaag atacaaattc taatcatcct tctgaaaata
    69481 ttgggattca gatagtatca agtggtccag atatatcaga acttgatgag attaaatatg
    69541 gttaccttaa aatgattcag aaagctagaa agtatatata catacagagt ccttatttaa
    69601 tacttgactc aacttttata gatactttaa aaatagcttg tttatctggt gttgatgtga
    69661 gagttatgat tccttctaaa cctgaccatc cctttgtata ctgggcatca tattcatatg
    69721 ctggagaact tttaaagttt ggagctaaaa tctacactta tggtcaaaat gcatttttac
    69781 atgctaagac aatagttata gatgactcta tatgctctat aggaacagct aatatggata
    69841 tcagaagttt tgagcttaat ttcgaagtta atgcatttat gtactcttca aaaaaagcat
    69901 tggaacaaag aattatattt gaaaatgata ttttaaattc taaggaaata acattagata
    69961 tatataattc taggtctact tatataaaaa tc
//
LOCUS       DAEPOZ010000002        67998 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.002, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000002 DAEPOZ010000000
VERSION     DAEPOZ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 67998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67998
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.002"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..305)
                     /locus_tag="KR440_000065"
     CDS             complement(<1..305)
                     /locus_tag="KR440_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0697140.1"
                     /translation="MKLYISVKTMLKVLKSSFMLNLIYFLALPLVLAGFLGMVTESIF
                     QNPIKTDLTSIVIYDKDNTKLSSNLTGYLKNDLSSILTVQKDDSKADLKLTIPKGYE"
     gene            complement(319..1254)
                     /locus_tag="KR440_000066"
     CDS             complement(319..1254)
                     /locus_tag="KR440_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0697141.1"
                     /translation="MDIVRVNNITKRFNDKLALDNISFSVKEGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGDIQINGLDLSKDCIKAKSIIGLVPQELALIETLTPFDNLEYFGT
                     YYGLKGKLLQERITEALEVTGLTDVKKKKIKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKNISKKQKTTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGD
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVVSILENNSLLELGVDKKLQLTDV
                     LSVVDKNNSKITRISYEEPSLEDVFLNLTGKNLRD"
     gene            complement(1442..2086)
                     /locus_tag="KR440_000067"
     CDS             complement(1442..2086)
                     /locus_tag="KR440_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0697142.1"
                     /translation="MNKIKVLIVDDEKLIRKGLNIILSSYDDLEIVGDASNGYEALEF
                     CKTNDVDIVLMDIRMKVCDGVLGTRLIKEYDSSIALLILTTFNDDEYIEDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMYPQKTIEQKHISDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILGKLELRDRTQLVIFAFKNKIVI"
     gene            complement(2079..3239)
                     /locus_tag="KR440_000068"
     CDS             complement(2079..3239)
                     /locus_tag="KR440_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0697143.1"
                     /translation="MNRKKTFIFIRYLSLFILLISYLNHTENTLYPAITMIILFLIIN
                     NQVRFFSLSSENKFILISYFLELILIFIFCKYIKTFNPLYYVSLILDASFLIKEKYKY
                     ILFSLVIIFSFIIGLDKNLYLALESSSTLLIITLFSVYLENENLSKLYNQSLYDKLRV
                     SKDELKKANADLEIYANSIEELAILKERNRISREIHDSVGHSLSTTIIQLNAIEKLLK
                     DKPLISDLVHELREFVTESFQDVRRAISELKPIEYENYQNLLKIRELVNSFIKLTNIN
                     VRLTISKNTWNLSTNQSIALYRLVQESLSNASRHGKATEIRIFITFNPSNLIITISDN
                     GIGCNNIKRGNGLSSISERIYELNGKVEFDNSNTGFTIRASFPKFSGGDFFE"
     gene            3424..3768
                     /locus_tag="KR440_000069"
     CDS             3424..3768
                     /locus_tag="KR440_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBH0697144.1"
                     /translation="MNNKEKGDFGEEIAVNYLLSKGAKILEKNYRLRIGEVDIIANIG
                     DEVVFVEVKSRSNIKYGYPCESVDFKKRKKIIGVAKYYIIKNNLNNTPIRFDIIEVYL
                     LEKRINHIMNAF"
     gene            3799..5400
                     /locus_tag="KR440_000070"
     CDS             3799..5400
                     /locus_tag="KR440_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBH0697145.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLLVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVSIMSFLDDKSRKENGELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISIEEMIEVSKV
                     YSILGMINESKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFNKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            5419..6519
                     /gene="dprA"
                     /locus_tag="KR440_000071"
     CDS             5419..6519
                     /gene="dprA"
                     /locus_tag="KR440_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBH0697146.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGYAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            6552..8639
                     /gene="topA"
                     /locus_tag="KR440_000072"
     CDS             6552..8639
                     /gene="topA"
                     /locus_tag="KR440_000072"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBH0697147.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            8804..9589
                     /gene="codY"
                     /locus_tag="KR440_000073"
     CDS             8804..9589
                     /gene="codY"
                     /locus_tag="KR440_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBH0697148.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            9696..10961
                     /locus_tag="KR440_000074"
     CDS             9696..10961
                     /locus_tag="KR440_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBH0697149.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDIEKGL
                     KRAVEVLNEDSYMDIECNNNAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            10992..11762
                     /locus_tag="KR440_000075"
     CDS             10992..11762
                     /locus_tag="KR440_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0697150.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDTLAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            11871..12317
                     /locus_tag="KR440_000076"
     CDS             11871..12317
                     /locus_tag="KR440_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBH0697151.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            12335..13528
                     /gene="nifS"
                     /locus_tag="KR440_000077"
     CDS             12335..13528
                     /gene="nifS"
                     /locus_tag="KR440_000077"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HBH0697152.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGRVDLRQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            13582..14022
                     /gene="nifU"
                     /locus_tag="KR440_000078"
     CDS             13582..14022
                     /gene="nifU"
                     /locus_tag="KR440_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HBH0697153.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            14311..15390
                     /gene="mnmA"
                     /locus_tag="KR440_000079"
     CDS             14311..15390
                     /gene="mnmA"
                     /locus_tag="KR440_000079"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBH0697154.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     misc_binding    15533..15771
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15839..18478
                     /gene="alaS"
                     /locus_tag="KR440_000080"
     CDS             15839..18478
                     /gene="alaS"
                     /locus_tag="KR440_000080"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBH0697155.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     gene            18503..18760
                     /locus_tag="KR440_000081"
     CDS             18503..18760
                     /locus_tag="KR440_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBH0697156.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            18770..20941
                     /locus_tag="KR440_000082"
     CDS             18770..20941
                     /locus_tag="KR440_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697157.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYGTMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNVGYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIININMIIFEKNN"
     gene            21147..21572
                     /gene="ruvX"
                     /locus_tag="KR440_000083"
     CDS             21147..21572
                     /gene="ruvX"
                     /locus_tag="KR440_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBH0697158.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            21650..21937
                     /locus_tag="KR440_000084"
     CDS             21650..21937
                     /locus_tag="KR440_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBH0697159.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            22219..22677
                     /locus_tag="KR440_000085"
     CDS             22219..22677
                     /locus_tag="KR440_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBH0697160.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(22794..23531)
                     /locus_tag="KR440_000086"
     CDS             complement(22794..23531)
                     /locus_tag="KR440_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBH0697161.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            23748..25424
                     /locus_tag="KR440_000087"
     CDS             23748..25424
                     /locus_tag="KR440_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBH0697162.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            25630..25830
                     /locus_tag="KR440_000088"
     CDS             25630..25830
                     /locus_tag="KR440_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HBH0697163.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            25884..27047
                     /locus_tag="KR440_000089"
     CDS             25884..27047
                     /locus_tag="KR440_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBH0697164.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(27258..27680)
                     /locus_tag="KR440_000090"
     CDS             complement(27258..27680)
                     /locus_tag="KR440_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HBH0697165.1"
                     /translation="MENKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(27701..28537)
                     /locus_tag="KR440_000091"
     CDS             complement(27701..28537)
                     /locus_tag="KR440_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH0697166.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            28835..29431
                     /locus_tag="KR440_000092"
     CDS             28835..29431
                     /locus_tag="KR440_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HBH0697167.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAGYVLIYSMGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGIYQIILSPIYTVLLKIVYLFV"
     gene            29461..30180
                     /locus_tag="KR440_000093"
     CDS             29461..30180
                     /locus_tag="KR440_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBH0697168.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKDKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            30182..30721
                     /gene="scpB"
                     /locus_tag="KR440_000094"
     CDS             30182..30721
                     /gene="scpB"
                     /locus_tag="KR440_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBH0697169.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
     gene            30883..31512
                     /locus_tag="KR440_000095"
     CDS             30883..31512
                     /locus_tag="KR440_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HBH0697170.1"
                     /translation="MELNELFNFKYLIIAVIFIISVTMLILISHINILIIADINNKDI
                     CLKLNIKYMFNLININRQLYPAENSKNNDKKEGMKNNIDSSILLADDLLSIYRLLKKI
                     KIHELYSNINFGTGNIGLTSSVYVLINTLYGNLFNMIDAEKMYLNVNPDFTKDYVLGN
                     IRIHIRPRIKALFNIIIMINKIMNKNKGNKEGDSNESNRFDTKSYGNNS"
     gene            31466..31894
                     /gene="ytfJ"
                     /locus_tag="KR440_000096"
     CDS             31466..31894
                     /gene="ytfJ"
                     /locus_tag="KR440_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HBH0697171.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            32142..32582
                     /locus_tag="KR440_000097"
     CDS             32142..32582
                     /locus_tag="KR440_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0697172.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
     gene            32598..34049
                     /locus_tag="KR440_000098"
     CDS             32598..34049
                     /locus_tag="KR440_000098"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBH0697173.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLVENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGSFVEGILLGNYKFDKYKTKSKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAIETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALTLLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(34140..34808)
                     /locus_tag="KR440_000099"
     CDS             complement(34140..34808)
                     /locus_tag="KR440_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HBH0697174.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSKNLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFRVNRYALRDNLAI
                     CFIYFSFIFLLNTFTNSQYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     KKSK"
     gene            35077..35217
                     /locus_tag="KR440_000100"
     CDS             35077..35217
                     /locus_tag="KR440_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697175.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(35392..35712)
                     /locus_tag="KR440_000101"
     CDS             complement(35392..35712)
                     /locus_tag="KR440_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBH0697176.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(35832..36569)
                     /locus_tag="KR440_000102"
     CDS             complement(35832..36569)
                     /locus_tag="KR440_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBH0697177.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            36784..38607
                     /gene="acd"
                     /locus_tag="KR440_000103"
     CDS             36784..38607
                     /gene="acd"
                     /locus_tag="KR440_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HBH0697178.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYIS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            38822..43120
                     /locus_tag="KR440_000104"
     CDS             38822..43120
                     /locus_tag="KR440_000104"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBH0697179.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEILPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            43324..43785
                     /locus_tag="KR440_000105"
     CDS             43324..43785
                     /locus_tag="KR440_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBH0697180.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            43815..44969
                     /gene="nusA"
                     /locus_tag="KR440_000106"
     CDS             43815..44969
                     /gene="nusA"
                     /locus_tag="KR440_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBH0697181.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVKSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELGTSEDNFSEEE"
     gene            44976..45257
                     /locus_tag="KR440_000107"
     CDS             44976..45257
                     /locus_tag="KR440_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBH0697182.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            45247..45558
                     /locus_tag="KR440_000108"
     CDS             45247..45558
                     /locus_tag="KR440_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HBH0697183.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            45580..47520
                     /gene="infB"
                     /locus_tag="KR440_000109"
     CDS             45580..47520
                     /gene="infB"
                     /locus_tag="KR440_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBH0697184.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            47563..47943
                     /gene="rbfA"
                     /locus_tag="KR440_000110"
     CDS             47563..47943
                     /gene="rbfA"
                     /locus_tag="KR440_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBH0697185.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDNLIKRVNEKNAQQNEDENYDE"
     gene            47924..48907
                     /locus_tag="KR440_000111"
     CDS             47924..48907
                     /locus_tag="KR440_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBH0697186.1"
                     /translation="MKIMMNNMDSIINYIYEGDYFIVTSHISPDGDNVGSTLSMYYTL
                     KNLGKNVYYVLDDEAPLNLRFLVEGVKIYKSNEFNMKNYSLIALDCGDKMRICVSDEI
                     KNNASKLICIDHHASNDSYGDLNYVDIDASSTCELVYNLLVRFQQTKDINIINEDIAT
                     CLYTGLVTDTGNFSYSNVHASSFEMAKNLLVLGAQKNTIIQNIYQSNSSDYYKLLGEA
                     LKGLEIFDSKVSSIVLTQDMMNRNNISFNDVDGITTYTRDIKGIEVGILFKEKKQNEI
                     KVSFRSKNYVDVSEIAKLFGGGGHVRAAGCTIRDSIDNAKKMVLEAVLKSI"
     gene            48920..49825
                     /gene="truB"
                     /locus_tag="KR440_000112"
     CDS             48920..49825
                     /gene="truB"
                     /locus_tag="KR440_000112"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBH0697187.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEVVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            49956..50885
                     /locus_tag="KR440_000113"
     CDS             49956..50885
                     /locus_tag="KR440_000113"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBH0697188.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEYAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            50989..51246
                     /gene="rpsO"
                     /locus_tag="KR440_000114"
     CDS             50989..51246
                     /gene="rpsO"
                     /locus_tag="KR440_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBH0697189.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            51486..52331
                     /locus_tag="KR440_000115"
     CDS             51486..52331
                     /locus_tag="KR440_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBH0697190.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            52456..54567
                     /gene="pnp"
                     /locus_tag="KR440_000116"
     CDS             52456..54567
                     /gene="pnp"
                     /locus_tag="KR440_000116"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBH0697191.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            54833..55576
                     /locus_tag="KR440_000117"
     CDS             54833..55576
                     /locus_tag="KR440_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBH0697192.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            55734..56981
                     /locus_tag="KR440_000118"
     CDS             55734..56981
                     /locus_tag="KR440_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBH0697193.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAICLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            57016..57261
                     /locus_tag="KR440_000119"
     CDS             57016..57261
                     /locus_tag="KR440_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBH0697194.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            57271..58494
                     /gene="dapG"
                     /locus_tag="KR440_000120"
     CDS             57271..58494
                     /gene="dapG"
                     /locus_tag="KR440_000120"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBH0697195.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEENIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            58607..59341
                     /locus_tag="KR440_000121"
     CDS             58607..59341
                     /locus_tag="KR440_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HBH0697196.1"
                     /translation="MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            59464..61875
                     /locus_tag="KR440_000122"
     CDS             59464..61875
                     /locus_tag="KR440_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBH0697197.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKSNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKSSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            61878..62936
                     /gene="mnmH"
                     /locus_tag="KR440_000123"
     CDS             61878..62936
                     /gene="mnmH"
                     /locus_tag="KR440_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBH0697198.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNEVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESVFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            62930..64264
                     /gene="rimO"
                     /locus_tag="KR440_000124"
     CDS             62930..64264
                     /gene="rimO"
                     /locus_tag="KR440_000124"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBH0697199.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFSELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHQIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            64251..64793
                     /gene="pgsA"
                     /locus_tag="KR440_000125"
     CDS             64251..64793
                     /gene="pgsA"
                     /locus_tag="KR440_000125"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBH0697200.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNVPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            65081..66127
                     /gene="recA"
                     /locus_tag="KR440_000126"
     CDS             65081..66127
                     /gene="recA"
                     /locus_tag="KR440_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBH0697201.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
     gene            66303..67844
                     /gene="rny"
                     /locus_tag="KR440_000127"
     CDS             66303..67844
                     /gene="rny"
                     /locus_tag="KR440_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBH0697202.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
BASE COUNT        27381 a         7244 c        12405 g        20968 t
ORIGIN      
        1 ttctcatatc ctttaggtat agtaagtttt aagtctgcct tagaatcatc tttttgaact
       61 gttaatatag aagataaatc gttttttaga tatcctgtca aattacttga tagctttgta
      121 ttatctttat catatataac aattgatgtc aaatctgttt ttataggatt ttgaaatata
      181 ctttctgtca ccatcccaag aaatccagct aaaactagag gtaaggctaa gaaatatatt
      241 aaattaagca taaaggagct ttttaaaact tttagcatag tttttacact aatgtacaat
      301 ttcatgaaat cacctccatt agtcccttaa atttttacct gttagattta aaaatacgtc
      361 ttctaaacta ggttcttcat aagatattcg tgttattttt gaattatttt tgtcaacaac
      421 agataataca tctgtaagtt gtaatttttt gtctacacct aattctaata atgaattatt
      481 ttctaaaata gatacaacac ctttagtatt ttttaaatca aatataagtt gagcatttat
      541 attttttatt tccataatta attttgtatt tgtagatact aatgatttta aataatcatt
      601 atctccaaaa gcaatctctt taccttcatc cataataaaa atcttagaac aaagatgctc
      661 aacttcttcc atataatgag aagtatatat tactgtggtt ttttgctttt tagatatatt
      721 ttttataaaa ttgaaaatat ggtttcttga ttgcgggtca acacctacag ttggttcatc
      781 caatattaat attttaggat gatttaaaag tgctattcca atgttcaatc tcctttgcat
      841 accaccagat aattttttga tttttttctt tttaacatca gtgagtcctg tgacttctaa
      901 ggcttcagtt attctctctt gaagcaattt tccctttagt ccatagtaag tgccaaagta
      961 ctctaagtta tcaaaaggag taagagtctc tataagtgct aattcttgtg gaactaatcc
     1021 tattatagac tttgctttta tacaatcttt tgacaaatct aaaccattga tttggatgtc
     1081 gccactattt ggtaacataa gatttgtaat tatatttata agtgtagatt taccagcacc
     1141 attcggtcca ataagtccaa atatctctcc ttctttaaca gaaaagctga tattatcaag
     1201 tgctaattta tcattaaatc tttttgttat attatttact cttactatat ccataataat
     1261 tcctcccaaa ataattttgt ataagttaaa aatctataat actataataa agaattatga
     1321 atcataattc tattactata agtcactaaa aaaagtgact taagtcactt tatattttca
     1381 tttattataa atgagtttgt gagtaagaaa tttctagggt atagatttct tacctatata
     1441 tttatattac aattttattt ttgaaggcaa atataactag ttgtgtccta tcacgaagct
     1501 ctagtttccc taagatattg gtgatattat tctttattgt accttcagat aagaataatt
     1561 cttggctgat ttctttgttg ttaagtccat ttgcgattaa tctaattatg gatatttctt
     1621 tttctgttaa attatacata tcagaaatat gtttttgttc tatagttttt tgagggtaca
     1681 taattttttc agccacactt ttatcaagta caatatttcc aaaaaacgag gaacgaatcc
     1741 cttcatgtaa tacttcatcc gaactatcct ttaaaagata tcctgaagcc ccaaatttca
     1801 tagcatcttc tatgtactca tcatcattaa aggttgtcaa aattaataat gctatagagc
     1861 tatcatattc ttttataagt cttgtgccta gaactccatc acaaactttc atccgaatat
     1921 ccataagaac tatgtctaca tcatttgttt tacaaaattc taatgcttca tatccattgg
     1981 aagcatctcc tacaatttct aaatcgtcat aagatgataa aataatgtta agaccttttc
     2041 taatcaattt ttcatcatct actataagaa ctttgatttt attcaaaaaa atcacctcct
     2101 gaaaatttag gaaaagacgc tctaattgtg aatccagtat tagaattatc gaattcaact
     2161 ttaccattta attcatatat tctttcgcta atactgctaa gtccatttcc tctttttata
     2221 ttgttacacc ctataccatt atcgctgata gtaattataa gatttgaagg attaaatgtt
     2281 ataaaaattc tgatttcagt tgcttttcca tgtcttgatg cgttggaaag agattcctga
     2341 acaagtctat ataaagcgat agattgattt gtagataggt tccaggtatt ttttgatata
     2401 gttaatctaa cgttgatatt tgtaagttta ataaagctat taaccagttc tctgatttta
     2461 agtaaatttt ggtaattttc atattcaatg ggttttagtt cagatatagc tcttctcaca
     2521 tcttgaaaac tttcagttac aaactctcta agttcatgaa ctaaatcaga aatgagaggt
     2581 ttgtctttta agagtttttc tatagcattt aattggatta tcgtcgtaga aagagaatgt
     2641 cccacactat cgtgtatttc cctcgatata cggtttcttt ctttcaaaat tgctaactct
     2701 tctatagaat ttgcataaat ctctaaatcg gcattagctt tttttaattc atctttagaa
     2761 accctgagtt tgtcataaag actttgattg tataatttag ataaattctc gttttctaga
     2821 tacacagaaa agagggttat aattagtaat gtagaacttg attctagtgc taaatataaa
     2881 tttttatcta aaccaataat aaaagaaaat attataacta aagagaataa tatatattta
     2941 tatttttcct ttattaaaaa tgaggcatcc aaaattaaag aaacatagta aagtggatta
     3001 aaagtcttta tatacttaca aaatataaaa attagaataa gctctaaaaa ataagaaata
     3061 agtataaatt tattctcact tgataaagaa aaaaatctaa cttgattatt tattattaga
     3121 aataatataa tcattgttat agctggatac aatgtatttt cagtatggtt taaataactt
     3181 ataagtaata taaatagaga taaatatcgt ataaaaataa atgttttttt tctgttcatt
     3241 aaagacattc ctccataaaa ataaactttc attataaaaa ctagcataat atcataaaag
     3301 aataaagtca atagaagtat ttattaatat caatggaaat aaaatcaatt tcaaaaaatt
     3361 gaatagttaa attgtatact gacgataatt taaaagtaaa aatttaatat attggagtag
     3421 ataatgaata ataaagaaaa aggtgatttt ggagaagaaa tagcagttaa ttatctgcta
     3481 tcaaaaggtg caaagatact agaaaaaaat tatagattaa ggatagggga ggtagatata
     3541 atagcaaata taggagatga agttgtattt gtggaggtga aatcaaggtc taacataaag
     3601 tatggctacc cttgtgaatc tgtagatttt aaaaaaagaa aaaagataat tggtgtcgca
     3661 aaatactata ttataaaaaa taacttaaat aatactccca taagatttga catcatagag
     3721 gtctatttgt tagaaaaaag aataaatcac ataatgaatg ctttttaaaa aagataatgt
     3781 ttttggaaag gattaaatat gttaagtatt attaattcaa gcaacttagt aggaatcgac
     3841 agctttttag ttaaggttga agtagatgta agcaatggaa tacctagttt taatattgtt
     3901 ggtcttccag gcaaagaaat taaagaagca agagaaagag taaagtcagc aattcttaat
     3961 agtggatata aatttccaag cacgagaata gttgtaaatt tatcaccagc agatataaaa
     4021 aaagaaggag cttttttaga tttatctata agtataggcc ttttaagaga gttaataaaa
     4081 aaagatgaaa attacataag agaaagtatg tttataggag aattatcatt agatggaaaa
     4141 ataagaaaag taagagggat attgcccata attatgggtg caaaaactca gaacataaaa
     4201 cgtattttta taccaattga gaacataaaa gaaagtctat tagtagatga aattgatata
     4261 attcctatta aaagtttaaa agaatgtgtc gattttttaa acgaagagat aaaagtcgat
     4321 aaagtaagta taatgtcttt tttggatgat aagtcaagaa aagagaacgg agaattagaa
     4381 aaagataata gttatataga ttgcaaatat actaagatta ataatgaaga aagtaaatat
     4441 gatgaagact ttaaagatgt aaaaggaaat tattttgtaa agaggagtgc agaaattgca
     4501 gcagctggaa atcataatat gtttatgatt ggacctccag gctcaggaaa aactatgatt
     4561 gcaaaaagag taaggacaat tttaccagat ataagtatag aagaaatgat agaagtaagt
     4621 aaggtttata gtatattggg tatgataaat gaaagtaaag gaataattga taaaagacct
     4681 tttagagcac ctcatcatac aactacaaag caatctttaa ttggaggagg aatggatgca
     4741 agacctggag aaattgcttt agcacataga ggtatcttat ttttagatga gatagccgaa
     4801 tttgatagaa aaatacttga gactttaaga caacctatag aagatggata tgtaaatata
     4861 tccagagtaa agtacagtgc aaaatatcct tgtagagtac tgttagttgc agctatgaat
     4921 ccatgtccat gtggttatta tatgtcagag acagagtgta gatgtagaag taacgaaatt
     4981 gatagataca taaataaaat atcaggtcca ttgttagata ggtttgatat ttttgtagaa
     5041 gttaattcta taaaatattc agattttaat tctttaaaac aagaagaaag ctctcaaaaa
     5101 ataaagagaa gagtagaaaa tgctagaaaa atacagataa atagatttaa gaaagataat
     5161 ataaaaaata atagtgaaat taaagcttac aatttattta aatattgtaa actggaaaaa
     5221 gaagcttcaa aaacggctga gatgatattt aataaataca acctaagcag tagaagttat
     5281 acaaaacttt taaaaatggc aagaacaata gcagatttag aagaaagaga cttgataaat
     5341 tcacagtgta ttatagaagc tttttcattt agaaaggctt attatagtta ttttaaatag
     5401 ttttttgagg tggattttat ggaaaaaaga gatacttatt tatggttaaa atcaattggt
     5461 ggaataacaa caaaaacaat tgaaataata gaaaatgaaa tagtaaatat tgaagatata
     5521 tttgattttt ctgaaaaaga aatatataat ttaaaaaata taagcttgaa tattagaaaa
     5581 aatatagtaa aatatagagg ttacgcttat ttagaaaata taaaagaact actttacaaa
     5641 aaagcgatta aatatatttg taaatatgat aaagaatatc ctgaaaattt aaagaatata
     5701 tacaacgctc caaagttatt attctacaaa ggtgacatag gtttagttaa taataatttt
     5761 aatatagcta tagtaggttc aagaaaacct acagcctatg gaattaattg tgcaaaaaca
     5821 ataagttgtc aactatccca atatggagtt aatatagtaa gtggattagc tataggaata
     5881 gatgcatact cgcacatagg ttgtatgagt ggaaaatcta aaactatagc agtacttggt
     5941 tcaggagtag acaatcctct tccaaaacaa aatttacatt tatcaaataa aatattagaa
     6001 aatgggggat tattattatc agagtataat attaactcga cagtagcccc gtaccatttt
     6061 tctaatagaa atagaattat cagtggttta agtgatggag tagttgttgt agaagcagca
     6121 ataaaaagtg gtgcactaat cacagtagat tttgctcttg aacatggaaa aaatgttttt
     6181 gcaatacctg gtaatataaa ttctcaaatg agtagaggtt gtcataaaat tataaaagaa
     6241 ggtgcaaaat taattgaaaa tatagatgat attttaaatg aatataacat atttaatatt
     6301 attgataaaa aaataaatca aaaatatgat aatataagtc taaatgccaa gtctaagcaa
     6361 ataattgaag ctataaaaag agaaggaaac ttgcatatag atagtatttg tgattatact
     6421 ggtatagaaa taaagtatgt aaattcaata atcaatgaat tagtactaaa tgagttagta
     6481 gtagaaatga ataataaaac gtacagttta aatgtataaa ttaagattag gtagtggggg
     6541 tgtatggttt tatggccaaa acattagtca tcgtagaatc gcctgcaaaa gctaaaacta
     6601 tagaaaaatt tttagggaaa agtcattaca cagttaaagc atcagttggt catgtcagag
     6661 atttacctaa aagtaaatta ggtgtagata tagaaaataa ttttgaacct caatatataa
     6721 atataagagg aaaaggcgat gtaataaaag aattaaaaaa agaagctaaa aaatctaaac
     6781 aagtatatct agcaactgac cctgatagag aaggtgaagc tatatcatgg catttagctc
     6841 atatactaaa tttagatgaa tctgaggatt gtagaataga gtttaatgag ataactaagg
     6901 atgcaattaa aaaagcaatt aaacatccta gaaatattga tataaatttg gttgatgcac
     6961 aacaggcaag aagagtgcta gacagacttt taggttatca aataagtcct atattatggc
     7021 aaaaggttag aaaaggtctt agtgcaggta gagtgcaatc tgttacaaca aagttaattt
     7081 gtgatagaga aaaagaaata aaggcattta ttccaaaaga atactggaca atagatgttg
     7141 aggctaagac agtaaaagac aaagatataa ctttgaaatt ttatggtaaa aatgatgaaa
     7201 agattgaatt agaaaatgaa gaagctgtaa atgaaatact aaaagaaatg gaaggtaaag
     7261 atttaaaggt tgagaagata gaatctaaat caagacgtaa atcagctcca aaaccattta
     7321 caacaagtat gcttcaacaa gaaggagcaa ataagttatt ttttactact aaaaaaacaa
     7381 tgatgatagc acaagaatta tatgaaggta tcgacataga aggtgaaggt actgttggtc
     7441 ttatatcata tataagaact gactctaaaa gaatatctga tgaagcaaaa gaaaaagcta
     7501 agggttatat cgtaaacaat ttaggagaaa actactataa aaacactggt gataaaaaag
     7561 aaaaaaagga tactaaaaag gttcaagatg cccatgaggc aataagacca acctcagttg
     7621 atagaactcc agagagtata aaagcctcat tgagcaaaga ccagtacaag ttatacaatt
     7681 taatttggag aagatttgta gctagtcaaa tggaagattc agtgtttgat atactaaatg
     7741 tagaatgtaa aataggcgat attgtattta aggcaactgg ttcaaaaatg aaatttgatg
     7801 gttataccaa ggtatataat ttttcagaaa gagaagacaa aattttacca agtatagaaa
     7861 aaggagatat attaaatata gaaaatatat tgccagacca acattttaca caaccaccag
     7921 caagatatac agaagctagt ttagtaaaaa cactagaaga actaggtata ggtagaccaa
     7981 gtacgtatgc tccaacaata gctactatac tgaatagaga gtatgttgaa aagcaaggaa
     8041 caagtctatg tccaacagaa cttggaataa tagttactga tattttggaa aataatttcc
     8101 ataaatttat agatgtagat tttactgctg atatggaaag taaacttgat gttatagaag
     8161 aaggcaatac agattggaaa aagattgtat cagaatcata tgcaccactt aaagaggcta
     8221 ttgaagaagc tcttgaaaat atagaaaaag taaatatgga tgaagaaact gatgagattt
     8281 gcgagaactg tgggtctaat atggttatca aatatggtag atttggaaag tttatggctt
     8341 gtaaaaacta tcccgattgt aaaacaacta gaccaattat gaataaaatt ggtgttaaat
     8401 gtccaaaatg tgaagaagga gatattatac ttagaaaatc taaaagaggt aaagcttttt
     8461 atgggtgttc taattatcca aactgtgact ttgtagcttg gaataagcca acaggagagt
     8521 tttgtgagaa gtgtggttca tatatggttg aaaaagtgac taaatctgaa acaaaaacag
     8581 tgtgttctaa taaagaatgt aataaagaga caaaagagca taatgacgga aaagagtgaa
     8641 aattgaaaaa aatcacataa ttctcttgaa aaaggttcca atctatgata atctttcaag
     8701 tgtaaacaac taatagaaaa ataaattttc attaaatgtg aaaataatat tagaaaatat
     8761 aaaaggttat taaaaggcct aaatttcatg aggagatgat taaatggcaa gtgaagtgtt
     8821 acaaaaaaca aggaaaataa ataaaacatt acaaacaagt ggtggaagca gtgtctcttt
     8881 tgatttactg gccggagcat tgggcgacgt tttaagttct aatgtttatg tagtaagtgc
     8941 aaaaggtaaa gtactaggtc ttcatttaaa tgatgttcaa gacagttcag ttatagaaga
     9001 tgagtatact aagcaaaaga aattttcaga tgaatatact caaaatgtgt taaaaattga
     9061 tgaaacatta gaaaatttaa atggtgagaa gatattagaa atctttcctg aagaacatgg
     9121 aagattacaa aaatatacta cagtagttcc aatattagga agcggtcaaa gattaggaac
     9181 attggtactt tcaagatatt caaattcatt caatgatgat gatttagtaa tagctgaata
     9241 cagtgcaact gttgttggtc ttgaaatatt aagagcaata ggtgaagaat tagaagaaga
     9301 aatgagaaag aaagctgtag ttcaaatggc aataggcact ctgtcctact ccgagcttga
     9361 agcagttgaa catatttttg ctgaattgga tggaaaagaa ggtctacttg tagcaagtaa
     9421 gatagctgat agagttggta taactaggtc tgtaatagta aatgcactta gaaaatttga
     9481 gagtgcaggt gtgatagaat caagatcatt aggtatgaaa ggtactcata taagaatact
     9541 taatgacaaa cttacagatg aattaaaaaa attaaaaaac aatcaataat ctatatttta
     9601 taggtttaga ttagaaaagg tatctatttc aaatagatac cttttttaga cgaattatta
     9661 aggtaatgat aagtatatta aaaaggagtg agaaaatgcc tttagcagat aaaataagac
     9721 caaaaacaat gaatgatgta ataggtcaaa gtcatataat aggtaatgga aaaatattgt
     9781 ctaagatatt acaatctaat tttttaccaa atatgatatt ttttggtcct cctggtgttg
     9841 gtaaaactac agtagcggaa ataatagcag aaagaagcaa taaaagcttc tataaaataa
     9901 atgctactaa ttcatcttta gaagatataa aaaaggtgat atcagagctt ggaagtataa
     9961 ataatgtaaa tggagtccta ttatatatag atgaaataca aagttttaat aaaaaacagc
    10021 aacaatcaat attagaattt atggaaaatg ggagtataac tttgattgct agcactacag
    10081 aaaatccata tcattatgta tataaagctc ttttaagtag gtctactgta tttgaattta
    10141 aacctttaga aaaacttgat atagaaaaag gactgaaaag agctgttgaa gtattaaatg
    10201 aagatagcta tatggatatt gagtgcaata ataatgctat tgaagatata gcaattcttt
    10261 ctgatggaga tatgagaaga gccttaaata ttttggaagt ggtagtctat tcaactaaac
    10321 caaataagga taatataact tatatagatt cagatgtaat aagggcatct acatttaata
    10381 aaattataaa ctatgacaaa aatggtgata gtcattatga tattttaagt gcatttcaaa
    10441 aatctataag aggaagtgac ccacaggcat ctatacacta tctagcaaga cttataaaag
    10501 gtggggattt gataagtata tgtaggagac ttttagtaat agctagtgaa gatataggtt
    10561 tagcttatcc aaatgctatt gtaattgtaa aggcatgtgt agactcagct atgcaacttg
    10621 gatttccaga agcgaaaata ccattagcag aagctactat tcttttggct acatctccaa
    10681 agtctaattc tgcatgtata gcaataatga aagctatgga tgaactggat aaagagtttg
    10741 ttaaggatat accaaatagt ataaaggatg ctcactattc tggttctaaa gaaataggta
    10801 ggggttgtgg atacaaatat cctcataatt ttaaaaatca ttatgtaaag caacagtatt
    10861 taccagatag cataaaagat agtatttatt acataccaca agaaaataaa actgagaaaa
    10921 atataaaaaa atatttagaa tatctaaata gtgattttta gactgaattt tatgtatcgg
    10981 gggtagatat tatgattata aaacttaact caacttatca cagtagagtt atgaaatact
    11041 taaaaaaaga acctgagtac aatttgttta taatagggga tattgaaaga tatggatatg
    11101 gaaataattt tttaaatata tgggcagacg tgggtgaaca tggtgaaata aaggcaatat
    11161 tacttaaata ttttgaattt atgatgtttt attctgatgg agagtatgat gttgaaggtt
    11221 tttataatct acttcgtaat actaattacg aagaaatatc tgggaaaata tgtgctgtag
    11281 atactttagc aaagagatta ggacttaata atttaaaggt agttgatttt tgcaaacttc
    11341 aaactaaaaa gtttttgata gataataatt gtaatgcaaa ggttaagaga attaggcttg
    11401 gaaatctaaa aaagacagtg aaactttatg acttaataga tgaatttcac agtacaactt
    11461 tagagaattt aaaaaatgga ctaaggacag gtagaggata ttgtatagag attaacaaac
    11521 aggtggtgtc aatggcaaag agtacttcag aaaataggac tcatgcaatg ataattggag
    11581 taggaacaca tcctaaatat agagctaaag gacttgctac aaaatgccta attaaactat
    11641 gttcagagtt actgagagaa aacaaaattc cttgtttgtt ttatgataat gaagaggctg
    11701 gaaagatata taaaaaacta ggatttgaaa acattggaaa atggggaatt tattcaaaat
    11761 aaaaaattaa aattaatctt gacaaaatta gtagggtata ataaaattat cttgaaagta
    11821 attcctacta aaacagtatg aattagattt ctatatagag gtgataatat atgaagttgt
    11881 ctactaaagg tagatatgga ctaaaagcaa tgtttgaatt agcattaaat caagacaatg
    11941 gaccagtatc attaaaattt attgctaaga agcaaaaaat atcagatcag tacttagaac
    12001 aaatattttc atctttaaaa aaatcgggtc tagtaaaaag tgtgagaggt gctcaaggag
    12061 gttatttgct ttcaaagaat gctgaagaca taacagtagg agatatactt gtagtattag
    12121 aaggaccagt tgcattatct gactgtgtac tagatgaaga tgtatgtgaa aactcaaaca
    12181 tgtgtgtaac aaaaatagtt tgggaaaaga tgaaaaaggg tatagaagat gttatagatt
    12241 caattactct taaagatatg ataaatgact ataacaaaaa taaattagaa aatgatataa
    12301 caaatattaa aaaatagatt tggagatgaa tgtaatggaa aaaagaagat tgtatatgga
    12361 ttattctgca acaacaccta ttaaaaaaga agtattggat gcaatgatgc catacctaac
    12421 agattatttt ggtaatgcat ctagttttca cacattcgga agagaagcaa aagatgcttt
    12481 ggataaagca agagaacaag tagcagccct tataaatgct gaacctagtg aaatatattt
    12541 tacagcaggt ggttcagaaa gtgataactg gacactagag ggtgtagctt atgctaataa
    12601 aaacaaaggt aatcatataa taacttcaaa aatagagcac catgcaatac ttcatacatg
    12661 tgaatactta gcaaaacatc atggatttga aataacttat ttagatgttg atagcgaagg
    12721 aagagtagac ttaagacaac tagaagattc aataaaagat actactatac ttataagcat
    12781 aatgtttgca aataatgaaa taggaactat acagcctatt aaagaaatat ccgaaatagc
    12841 taaaaaacat aagatattat tccacactga tgcagtacaa gctacaggta atattccagt
    12901 agatgttaaa gaattaggaa tagatttgat gagtatgtct tcacataaaa tatatggacc
    12961 aaagggtgta ggagctttat acataagaaa aggtgtaaga cttcataact ttgtccatgg
    13021 tggagcacaa gaaaaaagca aaagagctgg tacagaaaat atacctgcaa tagttggata
    13081 tggaaaagca gcagaattag ctaaggaaaa tatgcaaaat catgtagaaa ctttaactcg
    13141 tcttagaaat aagttaatag atggtgtgtt agaaagaata ccttacacaa gagtaaatgg
    13201 tagcttagaa aatagattgc caggaaatgc aaactttgca ttccaattta tagaaggtga
    13261 aggaatactt ttattattag atatgttagg aattgctggt tcaagtggtt ctgcatgtac
    13321 ttctggttca ttagaccctt cacatgtact tcttgcaata ggtttaccac atgaaatagc
    13381 acatggttca ttaagactta cagttggaga ttttacaaca gatgatgata tagattatat
    13441 actagagaac ttaccaaaag taatagaaag acttagaagc atgtcaccac tttatgatga
    13501 tgcgaagaaa caaggtttag taaaatagac agaaagataa agcataaaaa atagacataa
    13561 attgagtagg aggaaaagat aatgcaatat agtgataaag ttatggaaca ttttatgaat
    13621 ccaagaaata tgggagaaat tgataatgca agtggtgtag gtgaagttgg aaaccctaca
    13681 tgtggagata taatgaagat atttttagac atagatggag atgtaataaa ggatgttaag
    13741 tttaaaacat ttggctgtgg ttcagctata gcaagttctt ctatggcaac tgaaatgata
    13801 aaaggaaaaa ctataaaaga tgcattagaa cttacaaata aagctgttgc agaagctttg
    13861 gatggtttac caccagtaaa gatgcattgt tcagttttag ctgaacaagc tgtaaaagca
    13921 gctcttatag attatgctca aaagaacaat atccatatac cagaattgga tggatatgtt
    13981 atagatgatg ctcatgacca tgatgttgaa gaagaagaat aaaaataagt gattgtgtta
    14041 tgagaagttt acaatgatta aaaagaagta ctaatgatat aattaggaca taaaaaatag
    14101 ctgtttttaa aatggctatt ttttattgcc taaattaagt attttaactt gatttagagg
    14161 ttaattagtt aacaaaataa tccataagat tcaaataata aagatgatta tttttttaga
    14221 aaatagaaca agctatattt taaataagta caatacttgc tctatatggt ataataataa
    14281 gtaatgcaaa agatgaggtg aaatttgttt atgaataaaa aagtaatgat aggtatgagt
    14341 ggaggagttg atagctctgt agctgcatat cttttaaaac aacaaggata tgatgtcatt
    14401 ggtgttacta tgaagttgtg gcaagatgat gatgttgttg aaattgaagg tggttgttgc
    14461 tctttatctg ctgttgaaga tgctagaagg gtagcaaata aaataggtat accattttat
    14521 gttttaaatt ttagagaagt gtttaaagaa aaagtaatag attattttat agatgaatat
    14581 ttggaaggaa aaactcctaa tccctgtata gcttgtaata aacatataaa atttgatgat
    14641 ttttataaga aggctagaca aataggttgt gattatgtag ctacaggtca ctatgcaaag
    14701 atagaaaagg atgaaagtac aggaagatat ttgctaaaaa aatctgttac agataaaaaa
    14761 gaccaaactt atgcacttta taatcttaca caagagcaac tagagcatac tttattgcca
    14821 ataggtgact atgaaaaaga tagagtaaga gaaattgcta aggaaatggg gatggcagta
    14881 cacaataagc ctgatagtca agaaatttgt tttgttaaag ataatgacta tgcaaattat
    14941 gtaaaaaaac attctaaaaa gcgtattgaa gaaggttttt ttgtagatac taaaggcaat
    15001 atacttggaa aacataaggg tatattatat tatactatag gccaaagaaa agggcttgga
    15061 attacttttg gaaaaccaat gtttgtaata gacataaatc caataaataa taccatagtt
    15121 cttggagaca atgaagattt atttaaaaag gaacttattg ctaaagatgt taactttata
    15181 tcaatagata cgttagaaga acccttaaga gttcaagcca aaataagata ttcagcaaaa
    15241 ccatcaccag ctactattca tagagtagga gaagatacta ttaaaatagt ttttgatgaa
    15301 gctcaaagag ctattactaa aggtcaatct gtagtaatgt atgatggaga tattgtagta
    15361 ggtggaggta taatcgagaa aagtttataa aaacatctaa aaaattaaaa aaataagcaa
    15421 taaagtatat taagagtaaa taaagtttat aaaataaatt tattgtatat taaaatatag
    15481 tattgattaa ttttgtaaaa tttagtaata taattagtat attaattaaa aaacaaatac
    15541 agtgataagg aatagtaaaa tatttgatgt cttacagaga gagggaaatg ctggaagccc
    15601 ttagaatatg atatttgaag cagcctttta gtagcaatct tgaacaattt atcttttata
    15661 aagaagattt aagtagagat tgacggtagt tacgttatag ctaaggtttt ccgaagatat
    15721 catactttgt atgataatta gggtggtacc gcgattgtcc tcgcccctat aaatttattt
    15781 atagggacga ggttttttat ttaaacgaaa aaataaaatt tattaggagg aacatgatat
    15841 ggaaaagatg ggattaaatg aaataagaag taagttttta gaattttttg agtcaaaagg
    15901 tcattatgta gcaaatagtt attctttagt accaaacaat gataagagtt tgttacttat
    15961 aaactcaggt atggcacctt tgaaaaacta cttttcaggt gtagaagtac ctccaagtgt
    16021 gagaatgtgt acatctcaaa aatgtataag aacaggagat atagaaaatg taggtataac
    16081 tgcaagacat gctacattct ttgaaatgat gggtaacttc tcatttggag attactttaa
    16141 aagagagtca atcaaatggg gatgggaatt tgttacagaa tggctaaata taccagaaga
    16201 taagatatgg gtgactgtat atgaagaaga tgatgattct tatgatatat gggcaaaaga
    16261 aatgaatttc ccagaagaaa gaatggttag acttggaaaa gatgataatt tctgggaaat
    16321 aggtacaggt ccttgtggtc cttgttcaga aatatatttt gatagaggag aagaatatgg
    16381 atgtgacaat ccagactgta aaccaggctg tgattgtgat aggtatttag aattttggaa
    16441 ccatgtattt actcaatttg atagagatga agaaggaaat tatagtctat tagaaaacaa
    16501 gaatatagat acaggtatgg gtcttgaaag aatgggatgt attatgcaag gcgtagacac
    16561 tatctttgaa gttgatacaa ttaaatctat actagaagca gttgagaagt taacaggagt
    16621 aaaatatgga gagaatccaa aaaatgatat atcaataaga ataataactg accacataag
    16681 agctgtaaca ttcttagtta gtgatggagt gcttccatca aatgaaggaa gaggatatgt
    16741 tcttagaaga ctccttagac gtgctgctcg tcatggtaag ttattaggag taaaagaatt
    16801 atttttacag aaactaatag atgaagttat aaaggtaaat gataaagcat atccagtttt
    16861 agttgaaaaa gaaagttata ttaaaaaagt agttggaata gaagaagaaa aattcaatga
    16921 aactatagac caaggaacag aaatattgaa ctcttatata gaagtattaa aaaatgaagg
    16981 aaagactgtc ctaagtggtc aagaagcttt taaactatat gatacttatg gcttccctat
    17041 agacttaact aaagaaatat tagaagaaga acatctatct gtagatgaag aagcttttaa
    17101 tgaagaaatg gaaaagcaaa aagaaagagc tagaaatgct agaggaaata tggatggaga
    17161 aagttggaag gaagaccctc tatcaaaatt agaatctaca gttgacagta catttaatgg
    17221 gtatagtgaa atttatggtg aaggaactat tgaagctata gttaaagatg atgagctagt
    17281 tcaaagtgca gaagaaggcg ataaggtatc tatagtacta gataacacta ctttctatcc
    17341 tgaaggtgga ggacaagttg gagattgtgg attgataact aatgaaaact tagttttaga
    17401 agtattaaat actaaaaaag gtgctaataa tagtataaaa catataggta taataaaatc
    17461 aggaagaata agtaatgggg acaaagtaaa gacactcgta gatagagaaa ctagaatgtc
    17521 agctgcaaga aatcacagtg ctacacattt acttcataag gcattaagag aagtattagg
    17581 agaacatgtt aatcaagctg gttctttagt aactccagaa agacttagat ttgatattac
    17641 tcactttgaa gcgatatcaa atgaagaatt aaaagtaata gaagaaaaag taaataatgt
    17701 tattttatca tctttagaca taaaatgtga tattatgaat ataaaggaag ctaaagaaaa
    17761 aggagctaca gcactatttg gtgaaaaata tggtgatgag gtaagagtag tttctatggg
    17821 agattattct acagaacttt gtggaggaac tcacttgaca aatacttctc aagtaggaat
    17881 gttcaaaata ttatctgaag gtggagtagc agcaggtgta agaagaatag aagcaataac
    17941 aggaaaagct gtttatgaat acctaaaaga aagagatgga attatctctg aagtttgtgt
    18001 aaacttaaaa tctaaagaag ataatttaat tcaaagaata agttctttat tagaagaaaa
    18061 taaaaactta tctaaagaat tgcatgatat gaaagctaag atgagtttac aatcagttga
    18121 ttctattttt gattctaaag ttgaagtaaa tggagttaat ttgataacta ataaatttga
    18181 aggtatggat atggatacac taagagaaac agcagataat ctaagagata agcttggttc
    18241 tggtgtagta gtacttgcaa atgtagtaga tgataaagtt aactttgtag ttactgccac
    18301 taaagatgta ttagataagg gaatacattc aggtaatatt gttagagaag ttgcaaaaat
    18361 agctggagga aaaggtggag gaagacctaa tatggcacaa gcaggagcta gtgatgtatc
    18421 taaggttgac caagctttaa gctatgcaag tgaggttatt aagacacaag ttaaataaca
    18481 ttcactcaag gaggttttta gtttggaaaa agatttagac tacactatga aatttgaagg
    18541 aataccagaa gataggatga gtgtaggaga tactattgat tttgtatata aagcattagt
    18601 tgaaaaagga tataacccta taaatcaaat tatagggtat ctcctttctg gtgattctag
    18661 ctatataact agtcataaaa atgcaagagc tataattaaa aaatttgaaa gagatgagat
    18721 acttgaagaa gtaatcacgc actatctgaa tagaaagtag gtcaaataaa tgagaaaaaa
    18781 gattatgtcg ttgtttgcaa tagtcattat gatttttgta tcaactgcta gctatggttt
    18841 tgctgaagaa aaaggcaagg tcatatttat tgatatgaat aggaccaatc ttggaagtat
    18901 gatgaatatt ccaatcttga aaaatgagat agaaaagaga ggctatgtag ctctcatgaa
    18961 tataagaggg gatcaaggta ctgatgatag aaggtcttat gcaagtatgg gtgcaggaac
    19021 aagagctagt gtaactactg aagactatat caattttcaa aaagtagacc aagatactgc
    19081 taaagctttt gaagtcgcaa caggtcaaaa agctaaagaa ataaatgatt taggcattaa
    19141 tagatctata aatgaaaatc ttgagtatgg agaatatgga gcaaatttag ggtcattagg
    19201 aaaaacttta tcagaaaatg gtttaaagac atctgtaatt ggaaatgctg atataattga
    19261 aaataatcaa ttagttaaaa atagaaactt atgtttagta gctatggacc aatatggaag
    19321 gattgattca ggtaatattg ataatattaa taagaaagat tctaaaatgc catttggaat
    19381 atctacagac tacgataaat tactagatga aactaaaaaa tcctataaaa atagtgatgc
    19441 tatatttgta gaacttggag atacatatag attagatatg tatagattaa atctaaatga
    19501 taatacttat ggtacaatga agaaaaatat tgtaaaaaat ataaataaat atcttgaaca
    19561 agtattttct atggttggag aaaatgacac tgtttatata gcaagtgcat ttccaagtga
    19621 cctagattat aagaataaga gaagactttc tccaataata aaatttagtg gagaagggaa
    19681 aggtctactg gaatctgcca caacaagaaa ggctggaatt gttgccaatt tagatgttgg
    19741 agtagatata ctaggtacat ttggtcttga aaataaagag atggtaggta gaagttatac
    19801 tttagtaaat aaagaggaca atgttggcta tctaaaatca ctttatgaaa ggatagtgtc
    19861 tatatcttca ataaggtcaa ctattataaa tggctttgta ggcgtaatat ctgcttcttg
    19921 ggtaattgcc atgtttgcga ttttctttag aaattatatg ccacataaag agcgtgtatt
    19981 caaagtactt aaggagttta taaaattagg cataataatg ccactatcat ttatgttagc
    20041 tcctttgatg aattttaaaa ctccaatttc aatatctttg ggaatatttg cgactacgct
    20101 aatattatac ttgttaggta gaaaattatt taaggacaat gatataaagc acatgggatt
    20161 ttttgcaaca cttactattt tagtcatagt tatagattct gtatttggaa catacttaat
    20221 gcaaaataac ataatgagtt atgatgctgt cataggagct aggtactatg gtgtaggaaa
    20281 tgaatatgaa ggtgtaacta tagcaagtgc agtatttggc ttagcagtat tattacatta
    20341 taagaaggta tctaaattac ttgttgtaat tttttcgtta ataatactta taactagtgc
    20401 atatccttca atgggtgcaa atgttggtgg agctatatca gaatgtgtag catatttgtt
    20461 gtttataatg cttatatatg acattaaaat agattttaag aaagctgtat tattagctgt
    20521 ttcagctgta gttgtcgttt ttgcatttgc agctttagat atactttcag gtagtgaatc
    20581 tcatttaggt atgtttgtac agagaatttt cctaaatgga ccaggagaga tattacaaac
    20641 atttggaaga aaaattcaaa tgaatatgca actagcacag acaagtgtat gggtaaatat
    20701 tttacttgta ggaattggag taattgcagt acttatattt aaaccatcta gacactttag
    20761 aaagataatg aataattttc caatcttgtt taaagggttt attgcatcta tggttggttg
    20821 tataattact cttttagtta atgattctgg aatagttgct gcatctacag catctatata
    20881 tatacttata ccactaataa taataaatat aaatatgata atatttgaaa aaaacaacta
    20941 aaaaattttt tactgttcat ataggattta aataaaaaat gcaatatgat tattaatgta
    21001 aactggatgc ttacataaaa aatcatattg caatttttat gattgattat gcaaaattct
    21061 atgaagcagg aagcctgtga ccttagttat gagaggttca gaatatgtat ttaatctata
    21121 gaatataaat agataaagga ggaactatgt tagatggaag aataatgggt cttgatgttg
    21181 gtgataaaac tattggagtt gctgtgagtg atttgatggg attaactgca caaggtgtaa
    21241 aaacaataaa aagagttggc aagaaaaaag atattgaaga gttaaaagca ataataaaag
    21301 aaaaacaagt aaataaaatt gtatcaggat taccaaaaaa tatgaatgga actttaggtc
    21361 cacaaggaga aaaagtaatc aagttttgtg aattggtaaa agcagaaaca ggtattgatg
    21421 tagagttttg ggatgagagg ctttctacag tagcggcaga gaggtcttta ttagaagcag
    21481 atgttagtag acaaaaaaga aaaaaggtta tagatatgtt ggcagcagta ataattttgc
    21541 aaggatattt agattttaaa ataaattcat aaaagaacta taaaattgaa tacttatgat
    21601 atataatata aataataaaa ataaaaataa atttgaggtg aatgacatta tggaagaaaa
    21661 tataataaat ttaatagacg aaaatggcgt tgaaagccaa tttgaaataa tattaacatt
    21721 agaagcagaa ggaaaagagt atgcaatatt aatgccttta gatgatgaag aagctgaaga
    21781 agctttaatc tttagaattg atgaggatga agaaggtgaa atattaatac ctttggaaag
    21841 tgatgaagag tatgaaactg ttgtagctgt gtatactgct ataatggaag aagaaggttt
    21901 aaattacgac gaagatgaat caaatggact taactagata agtaattaag tttgtatata
    21961 aaaatcagat atttattata tttgctttaa ttaaacctta ttaattgtaa aagcatttat
    22021 agtatatatc tgtttttttg ttttttatta aaaataccaa aaaatttatg aattttggtg
    22081 aataatatat cttttatggg tatatattta tattatgaga ataaattaaa tttatttaaa
    22141 tgtcttgaca aaaaatgaat tatcaattac aatattattt aataaagatt atcaattgaa
    22201 aatagtaagg gtgatgttat ggcaaataca atggatttat taaaagacaa gttaaaagaa
    22261 actggtttta agattacgcc acaaagaaga gcaatagttg aaatattgct taaacatgac
    22321 cattcacatc taagtagtga ggaaatctat gatttagtta gggttgactg tcctgaaata
    22381 ggcttagcta ctgtatatag aacaatgcag ctattggatg aaataggatt aatatcaaaa
    22441 ctaaacttag acgatggatg tataagatat gagattagtc tacataaaga agactgtcat
    22501 aatcatcatc atcttatttg taaaaattgt ggaaagataa tggaagctaa agaagacctt
    22561 ttagacaata tagaaaaaga gatacaatca ttgtacaaat ttaaaatact tgatcatgat
    22621 gttaaatttt atggattatg tgacgagtgt aatggagtaa gcgattcaga agaataaact
    22681 tctattgctt aatgtcgatg gaagaatagt atagaacttt tatattctat gctattcttc
    22741 cattttttat tataaaccat ctacaaaatg caaatataca gttttcaaga tgcttatagt
    22801 tttaatattg aaaatataat taatcctata agtacaaaaa tgaatgattt ttctatgaaa
    22861 ttactaaatg gtcctccaac agttaaaaaa cctaaagaaa ttttcttttc gcttagcgga
    22921 taaaaaattg gtattccttg ctttgtaaat aaatctccta aaaacatatg acttgcataa
    22981 ccaatcgtac cataaaaaga taaattatta atattagtga ttaattctat ctgttttagt
    23041 aaaaaatata ctattatagt tgcaaatatg ctatgagaaa aacttctgtg ctttaaccag
    23101 ggaaaaattg acaacattag acaaaaaatt gtaaaatata tttcaacttc tatgatgtat
    23161 aagcatatag ctaatagtat aaatattaat gatagaaaaa cttttcttag aaatgtgtgg
    23221 gttagttttg cttcaataat tataattgca acaaatgtaa ttattgcgct taaataaaaa
    23281 ttatcattta tatttattga aataaagaaa ataataatat taacagcata tataaaaatt
    23341 ctatatatgt atttggatac attgtttttt agaatgaaat ttgagaccat agaattagat
    23401 ttatccaaat cgggtagtac agcaaaagta gctgaaacta aaatatcaac taaagatata
    23461 ggaattttaa atattaaaga tgtttggata gctgttaaga ttcctattgc acaatgagtt
    23521 ttaccacgca taaatcctcc taaattatcc gatattaaat catataatat ttagaggata
    23581 tcaaaataaa ataggtttgt caaaataata cgttaaatgt attttaaata tagcttaaaa
    23641 tatgatacga attatggtac aataagaata actggtagcg agtgcgtgat ttgatattat
    23701 ttatgattgt agaacgtgtt aaaaaattaa atagacagaa ggagatgatg caattgttca
    23761 aaaagaacac caacaagata aaggtaatgg ccttgggtgg acttaacgaa gttggaaaaa
    23821 atatgactgt tgttgagtat aaagatgaaa taattgttat agatgctgga cttagttttc
    23881 cagaggatga aatgttagga gtagatatag ttataccaga tataacttat ttagtaaaaa
    23941 atagggataa aataaagggt atatttatta cacatggaca tgaagaccat ataggagctc
    24001 ttccatatat attaaagaaa ataaatgtac ctgtatatgg ggcaagactt agtataggtc
    24061 ttatacaagt aaaacttaaa gaacataaaa tgaataatgt taagttaaat gttataggtc
    24121 ctagacaagt tataaagctt gataatatgg aagtagagtt tttgaaaaat aaccatagta
    24181 taccagatgc ttactctatt gcaatacaca cagaccaagg tataatatac cacacaggag
    24241 attttaagat tgacttgact cctattgatg gagatgttat ggatatgcat agaatctgtg
    24301 agttgagcaa aaaaggagtt cttttaatgc tggcagatag tactaatgca gaaaaaccag
    24361 gatttactat gtctgaaaag actgttgggg taggtcttga cgaattattt gcaaaaggaa
    24421 atggcagaag aattatagtt gctacctttg catccaacat acacagatta caacaaataa
    24481 taaatactgc tgaaaagttt aatagaaaag ttgctatatc aggtcgctca atggtcaatg
    24541 tagtcggagt tgcaaaagaa ttaggatacc tagatatttc tgatgacatg cttatagatt
    24601 taaatgatat atgtaagtat gaagatagtg aattagtgat aataacaact ggttcacaag
    24661 gtgaacctat gtcagctctt gctagaatgg ctttttcaga gcacaaaaaa gtagaaataa
    24721 agagtggcga tttagttata atatctgctc atccaatacc aggaaatgaa aaattaatat
    24781 caagagtaat caatttctta tttgaaaaag gagcagaagt agtatacagt gatatagctg
    24841 atatacacgt ttctggacat gcctgtcaag aagaattaaa acttatacat gcattagtta
    24901 gaccaaagtt ttttatgcca gctcatggtg aatatagaat gttaaaaaga catgctgaaa
    24961 tagctgaaca gcttggtatg gataaagaaa atatttttgt catgcaaaca ggagatgttt
    25021 tagaattaga taaaaactct gctaaggtag caaaccgtat acaaacagga aacatattag
    25081 ttgatggtct aggagtaggc gatgtaggta atatagtttt aagggataga aagcatttat
    25141 cagaagatgg tcttatgata gttgtagtta ctatttccaa ggatgagggt aaggtcttag
    25201 caggtccaga tataatttcc agaggattcg tatatgtaag agagtcagaa gatttaatgg
    25261 atggagcaaa ggatataatt aagaatgttc taaatgaatg tgaagagaaa aatattaaag
    25321 aatgggctta tttaaagaat aatattaaag aaaacctaaa agaatattta tatcaaaaaa
    25381 ctaaacgaaa tccaatgata cttcctatca taatggaagt ataatataaa aaaactataa
    25441 atttgaaaat tagacctctt gtaattttta aagtaatatc aaaaatccaa ttgatattat
    25501 cttaagaatt acaagaggtc tttttaatac tcatttaaaa aaaatatata ttacataaaa
    25561 taccaattat aacaatcttt tattagtaaa aactacttat atataaattt tactgatagg
    25621 aggattctta tggatgatgt ttcaagacaa aatgctataa aagctttaaa acaaacaaaa
    25681 atggaaatag caggagagta tggaatgaat tatgaagatg cttttgaaat aatagaaaat
    25741 gcgtctaata aaggtgtttt agaaggttac tttaaaaagc ttgaaaagaa aaaaaattta
    25801 ggtcaaggta tatctaggca tttagaataa aaaaagttaa atgtgagaaa ataagtttaa
    25861 ataaacttta agaggtgaca tcgatgaaaa aattagctag tactgctttg gctatattaa
    25921 ttgcgcttac tcctttaagc ttttcttttg caaataataa ggaaaatgct gatgccaacc
    25981 aactaaatat atcttcaaaa tcagcaattt tgatggatgt tggtagtgga caaattttat
    26041 atgaaaagga tgctcataaa aagcttcctc ctgctagtgt aactaaggta atgactatgc
    26101 tacttatagt agaagctttg gattcaggca agattaagct tgatgatgaa gttcaagtaa
    26161 gtgaaactgc atctagtatg ggtggtagtc aaatattcct agagccagga gaaactcaaa
    26221 aggttgatac attattaaaa ggtatagctg ttgcttctgc aaatgatgct tgtgttgcta
    26281 tggctgaaca cttagctgga agtgttgaag gatttgttga tagaatgaat gctaaagcaa
    26341 aagagttaaa tatgaatgat actcattttg caaatacaaa tggacttcca gttgcaaacc
    26401 actatacatc tgctcatgat atagctttaa tgtctagaga actattaaaa cacgaaatga
    26461 taagtaaata cttaactaca tggatggata aggttgtagt agggaagaaa caagttacag
    26521 taggtcttgc aaatactaat aaacttataa agcattatca aggagcaact ggtgttaaaa
    26581 ctggttttac tcaagaagca aaatactgtt tatcagcatc tgcaaagaga ggtaatactc
    26641 atctagttgc tgcaacttta ggagcagaga cttctccaga aagatttaat gatgcctcta
    26701 gccttttaaa ctatggattt gctaattatg aaagtgttaa gttatgtagt aaaggtgata
    26761 atatagcaac acttacattg gataaggctg atgaaaacaa agttaaattg gttgctaaag
    26821 aagatttaaa tgctcttatt aaaaaaggaa gctctaaaga atttgagaag aaaattgaaa
    26881 tagtaaaaaa tcctaagatg ccaattaaaa agggaactgt tttaggaaaa ataaagatat
    26941 gtaaagataa aaaagtaata ggcgaagtgg aattgataaa tactaaggat ataaataagg
    27001 caagctatct acaaatgctt caaagaataa ttgataatat gatataaata attatcagat
    27061 gaaatataat tataatacac aaagatatga ttataataca cataaaaaat acccttatgt
    27121 agttatttgc cataggggta tttttattta ccatatagaa gtattttata atcttatgat
    27181 ttttatagaa atatagaaaa gtttatgtat cttaaaatat catttgattt aatatcatat
    27241 atagggttgt ttaacaatta tctaagtaaa ggcatactac cagtaagttt attcactttc
    27301 tttttattcc cataaattgc tatcccaaaa tactgtaact catcttctgg aacactagca
    27361 atcttttgtg taaattcttc ataaagattg caactttgtg ctacatctga aaaatctaca
    27421 acagttaaat cgttaaattc caaagtatat aattttttac gtaaatcttt tattatttct
    27481 ttgtttcctt ttaaaatagg tacaggaatg tcaataattc ctaagtggct attacctgtt
    27541 ttgtcaatta catctggccc aacagtttct ggggcatgtt ttcctagtgt aattcccatt
    27601 atagctgctg tattggatat aattcccatt ggtaaatttt cgtctattac catgacacat
    27661 tttgaatttt tattttccat ttttatttct cctttttacc ttatttaaag ttgtttgaat
    27721 catcatttat aaatatattt tgatactgct ttggtgtaag accaataaaa ttcttaaaaa
    27781 agtttgtaaa gtgactttgg tctacaaatc ctgtttgtat cgcagcatct ataggttcta
    27841 cgcctttttc taaaagcttt ttagctttgt ttacccgtat attttctagg tatctatatg
    27901 gtgtaatacc ttttaatttc gtaaaattac gaagtagaga gtacttattc atacctgcta
    27961 tagtgcttaa ttcgtcaagt actatatgtt cagcataatt attttctaga taatcacata
    28021 ctgcttgaat ttctatattt gtattttcca agttagattg caaatttggc tctgtatgtt
    28081 cttcaattaa ttgctcaatt aggaagaaaa aaagctcttc ctttttaaaa tccaattctt
    28141 cttccatgat tatatagtgt aattcttgta gtagaggtac tagctcactg cgaaatataa
    28201 caggttgatt aaattttggt aaataatttt tacctgtaat ttcaaagacg gtttttttca
    28261 ttatttctgg cttaatattg atacatctgt aatcaagtac tttatcatct atttgttcac
    28321 aagtatgact gtcaaaaggg ttaaaaagta gcaaatctcc agtacttgtg gtatactctt
    28381 tatttttaca agataaatat ctttgacctt tttctataaa accaattaca taatgttcat
    28441 gaaaatggtt tgggaatttt tgcattatgc ctttaaatct atatgcttca atttttaatt
    28501 ccctatcaaa gcatactgtg cgttcttctt gtctcactta ggtaacctcc tttcatgtta
    28561 tacaaagtgt aacacatatt tttttatact tcttgtataa tattgactaa gagacaataa
    28621 tgtcaattca tgtcaatata atattacact tacagtaggt gtaattatta tattttcaca
    28681 atgaatatga tataataata aaagttataa aataaaaagc aaattttaat tttatgccta
    28741 gaagttaaaa ttattataat gtaaaataat tttgattata agctatttaa ttgttataat
    28801 aatattaaaa atttaattta ggagatgtac attaatgtta agtatgaata taggagaaat
    28861 agtagcaagt ttagttggta tagctatggc aatatcaata catgagtttg gccatgctta
    28921 ttcagctcac ttattgggag atgatacagc taaagcttat ggtagaatga cattaaatcc
    28981 tgtaagacat gttgatccaa taggtctttt ggctatgttt atccttaaga tagggtgggc
    29041 aaagccagta ccagtaaatc caaataattt taaaaactat aagataggaa acctgatagt
    29101 ttcacttgca ggcgtttttt gtaatgtttt gactgctata ctgtgtgtaa ttatcaataa
    29161 gtatgtgcat atgtatgcta taaatacaat agctggatat gtgcttatat atagtatggg
    29221 atttgcagct tttaacttac ttccgatacc tccacttgat ggatggggag ttatatctac
    29281 atttgtacca tataaatgga atgaatattt gtataaattt gaaagtatga gttatataat
    29341 tttattaatt gctttattta ctggtatata tcaaataata ctatcaccaa tatatacagt
    29401 gttgctaaaa atagtatatt tatttgttta atataataaa cagattaaag agggattgat
    29461 atgaagtata atatacaatt acaagtctat gagggaccat tagatttact ttatgattta
    29521 ataacaaagc ataaaataga tataaaggat atatctataa tagatataac taagcagtat
    29581 ctaaattatt tgaaaatgct agataaaatg gatttagaga taactagtga atttataact
    29641 atggcatcaa aacttttaga gataaagtct aaatatctgc tttataagca aaaagatgaa
    29701 gaagaagacc caagaataga acttatggaa aagttagaag aatatagaaa gtttaaggta
    29761 gcatcacaag atataaaaga aaatataact tatgttaatg agagatttta tagaaataaa
    29821 gaagagataa ttattgatga caatgtggat ttagaagata tatcaattga agcaataaaa
    29881 aatatacttc catacatatt taaagttaaa acatcacaga tagaaaatgc taatgatgag
    29941 aaactagaca aaatagtaag gaagaagata atatctgtgg aagaaaagat attgtatata
    30001 agagatatta taaaagataa gattgaagtt acttttacaa atattataaa atcatatgaa
    30061 aatgatgaaa taatagctac ttttttatct atattagagc ttataaaaga aaaagaaata
    30121 gtagtagtac aagacatatt ttttgatgat attctaataa gaaaaagttc ggagtgttaa
    30181 aatgaaaaga gaagatataa agtatattat agaatctgtt atgtttgcat atggggaacc
    30241 aataagtata aaagaattaa actatataat aaataaagaa ctttcatcaa aagaaataga
    30301 gattatgtta aatttactta ttgaggagta cagagaacaa aatagaggta ttcaaataat
    30361 caagctagag aataagtacc aaatgtgtac aaataaagac tatgcagaat atataaagaa
    30421 gattattgaa ccaaagaaaa agaaaagttt gagccaagct acattagaaa ctcttactat
    30481 aattgcctac aaacaaccaa taacaaaggt agaaatagag gatataagag gagtaaaatg
    30541 tgataaggta ttacaaactt tgtttgaaaa tgaacttata agagaagctg gtagattgaa
    30601 taagataggg aaaccaataa tttataaaac aacagatgaa tttttaaagt tgttgaatat
    30661 agaaagtctt gaagaattgc cgcctataga aaattatcaa gaagtggcaa caaatgaata
    30721 aaaatataaa atcacaataa tttagtatgt aattttaaat tttaaaagat ttaaaatgct
    30781 ctaagttgtt gtgttttttt gtataaatat aaaattataa ttctcttaaa ataaatatgg
    30841 ataattaagt ataaatagta attttttgat gataatataa acatggagct taatgaatta
    30901 tttaatttta agtatttaat aatagctgta atttttatta taagtgtaac tatgttgatt
    30961 ttaatatctc atataaacat cttaataata gctgatataa acaacaaaga tatttgttta
    31021 aagcttaata taaaatacat gtttaattta ataaatataa atagacaact ttatccagct
    31081 gaaaatagta aaaataatga taaaaaagag gggatgaaga ataatataga ttcaagtata
    31141 ttattagctg atgacctttt gagcatttat agattattaa aaaagataaa aatacatgaa
    31201 ctttattcaa atattaattt tggaactgga aatattggct taacatcttc tgtatatgta
    31261 ttgataaata ctttgtatgg aaatttattt aatatgattg atgcagaaaa aatgtattta
    31321 aatgtaaatc cagattttac aaaagattat gttttaggaa atataaggat acatataagg
    31381 ccaagaataa aagctttatt taatattata ataatgatta ataaaattat gaataaaaat
    31441 aaaggaaata aggaaggtga tagtaatgag agcaacaggt tcgatacaaa gtcttatgga
    31501 aacaactctt gaaacaatta aaggttctat agatgcaaat actattatag gagaccctat
    31561 aaaaacagat actacagtgg ttgtacctat atcaaaggtt acaattggct ttggtatagg
    31621 aggaggagaa tactcaaaag gatatgatga taaagatagg gaaattgaat taaaaaatga
    31681 aaaatcagat acgaattttg ctggaggtag cgcaggggct atatctgtac aaccagtagc
    31741 atttgtagta gttgaaagtg gagagactag aataatgagt ttagacagta atatcaatct
    31801 agtggataat atattatcta taactcctag agtattggag aaaattcaaa atatatcaca
    31861 aagtaataag aatcaagata aaaacaatat gtaagaataa aatattttta aatatgtata
    31921 tagttttaag taaagtagtg gggttagtat aaatcccact attttgttgt gtactttaaa
    31981 catttatatc taataagatt ttagtttaaa acaatttaaa tagataataa agttgatata
    32041 tagtataatt ataaaaagaa aagaagtatt atattataga tatataaacg catttgagat
    32101 ataagaggga gttataaatc attagttttg aggtgaatgt tatgagcgat aaagaattat
    32161 ctattttgtt gttagaaagt atgaataatt ttcataattt aataaagatt attaataacg
    32221 aaagatataa aaaagataag gttcttacag aaagacagtt ctttgcctta gtaaaaataa
    32281 gaaaacatga taaaatagag cttaaaaatc taagtagaga tttacatgta tctacttcta
    32341 gtctgtgtat actacttaat aaattggttg agcaagagta tgtatatagg gaagaagata
    32401 gtagagatag acgaaatact ttttatggaa taactaaaaa tggagaaaag atattggata
    32461 atgaaatact aaagtttgta tctataataa gtgataaaat ggattgctta gatatagata
    32521 ataaagataa attatttact tctttggaag agtctaaaaa tataatagaa caattatttt
    32581 aaaaaggaga atttattatg aatataaagt attctaaaga gatattaaaa agtttagaaa
    32641 atgctataac agtagtagct ctatttgagg gagaagatgt ttctacgaca tttgataaaa
    32701 taaatatgat aagcagaaat tttaattcta aagaaaatag tatagatact gtaacattgg
    32761 ttgaaaataa caacttggta aatgtttgtc ttgttggttt tggtaagaga gagttattga
    32821 atagagaaaa aattagaatc ataggtggta atttgtgtaa aaaattaaaa aatatattga
    32881 aaaatgaagc atataagagt agtgatgtta atgtacttaa tcttaattta aatagtgatg
    32941 aaataggttc ttttgtagaa gggatattac ttggaaatta taagtttgat aagtataaga
    33001 caaaaagtaa ggattttgaa agaaaagaag ttgatactat aactatattt actgaaaaag
    33061 aagttgagtg tcaaatagaa aaggctaaaa tattagctaa ttcaactata atagctagaa
    33121 atctagttaa tgaaccatca aacgtaatat atccaaaaac attagcaata gaaacagtta
    33181 aacttggtgc agagttcggg tttaatgttg atgtttatga agaagaaaaa ataaaggcat
    33241 tgggaatgga tgcttttttc gccgtttcaa gaggttcaat caataaacca agattcatag
    33301 taatgagata ctttggagat aaagaaagta cagatatatt aggtctagta ggtaaaggat
    33361 tgacttatga tactggtgga tattctttaa aatcaaatgc ttctatgcta gatatgaaga
    33421 cagatatggc tggtgctgcg agtgttatag gtgctatgtg tgctatatct caaagtaaat
    33481 taaagaaaaa tgttatagca gtagtggctg catgtgagaa tgctttatca ggaggttcat
    33541 acaaacctgg agatataatt agttctatgg caaagaaaac tattgaagtt ttaaatacgg
    33601 atgccgaagg tagacttaca ttagctgatg caatatacta cattataaac aatgaaaaag
    33661 taacaaaggt agttgatgta gctacattaa ctggtgctgc tttaacattg ttaggaaatg
    33721 ttgcaactcc tatagtaaca aataatgatg atttttattg tgaattagaa aaagctgcca
    33781 ctttatcagg agaaagggta tggaagatgc caatttatga tgaatttaaa gatatgataa
    33841 aaggagaaga agcagacctt aaaaacactg gtggtaaaaa tgctggatgt ataacagcag
    33901 gagcttttat tggtgaattt gttgggaata ctccttggat acatatggat atagcgggga
    33961 caagtacatc atcaaaatca attggataca aagcaaaggg ggctacagga gaacctgtta
    34021 gaacattata ctatttggca gaaatctaaa aaacaattat aaaatataat ttataaagcc
    34081 acatggatat ttttgtaatt taagattatc tatgtggctt tattttttat ttaagtaaat
    34141 tatttactct ttttcttttt ctcaagtata aaagtatctc tattaaataa aagagcaggt
    34201 atgtacataa tactagtaac aaaaataaac gtaaaaatag cagttgtgct atgacttaaa
    34261 aaaccataat agtattgtga gttggtaaat gtgtttagta aaaatatgaa tgaaaaataa
    34321 ataaaacata tagctaagtt atctctaagt gcatatctgt taactctaaa ttttctaaca
    34381 tcaaccatat atatatctgc ataaattatc aagcacttag agaagataaa tataaaatat
    34441 attaagctag gaaattcata gcccaagttt ttagaaaaaa tgagttctcc aactgaacaa
    34501 actaaactcc aagaaaaaaa tatattaaaa agatgatact gcttaaaaat tagtatggct
    34561 atacacagat atatacagat ataattgatt ccaattggta aagagtttag agtactataa
    34621 ttacctgaag aaataagtaa taattgctct agagccataa ttattacaag taatgtacaa
    34681 attatcttct caactgtata gctataaggt aaaatatttt tggttagttt tggacaatag
    34741 tataaaaata atccaaatat aaagagactt acaaattgtt cactagaaaa aagaaaaatt
    34801 ttaaacatat aacattctct cctataataa atttaatatg tatctatata aatatattca
    34861 ttatatctca atttaatata taaaaaagat aataatagtt agacttttta tatgaatttt
    34921 aataggtaaa atatcctatt gttttgtaga tatatcctaa ttatgtcaaa aaataaaaaa
    34981 atttttattt attagagttt ttggaaataa taataacaga tattataatt aattaacagt
    35041 ttttgtaagt atttgacaat agagagggtg taacaaatgg gaataataga gtcagcatca
    35101 aaattggctg agatggttca tttattggct gttgaaaagg ggataactga tatagaagca
    35161 tgggatgaag ctgtgaaaga atacagcaaa atttatgaag aaagaagaaa tgaatagaat
    35221 gataaaaaat gaaaacttga tgataaaaaa taactttatt taaagaaatt caatttttat
    35281 agaaatatta aaaataatat aaataaaaag atggtatttc aaatgtaagt aaaaaatcat
    35341 tttgggatac catccttatt atttatttta ataatatata agtaataaat cttacttatc
    35401 catatttttt atgtaacctc ttttttctag cttttttata tgctccttac gtatttcatc
    35461 aagattaata ccatattgtt cttctagctt aaacatcata gtaacacatg tctgagctac
    35521 atccattagt tccttagcag ttttctttat aatatcaact tcatctaaat cattattttc
    35581 tccacttaga tttctgaatt tacctataca ttcagctaat tcaccttgtt cttcacccat
    35641 tttgataaat gtagattcta gtgttgggtc taaattatta agctctggta aagatatagt
    35701 ttttagtttc ataaaagctc ctttcaagtt attcgtattt atactaaaac atcataacaa
    35761 aaataatact aaaaaaaaac tttaaattca acataaaaac ttacatatat aataaaaatc
    35821 gtgaaataaa tctatatttg tcttaataca gcctcatata aaacttctcc aataggttta
    35881 tttattacaa gagaagctga cttatcatag actgtatttg ctttattaat aagagcaata
    35941 taatcacctt tgtaataatc tataaaactt gctgctgggt acacagctaa tgatgtacct
    36001 cctataatca ataaatcagc attacttata gcagaaattg ttttagtaat gacatcactg
    36061 tcaagagctt cctcataaag tactacatct ggttttacta tagaaccaca gttgtcacaa
    36121 taaggaatat tacctcccag atttaacata gattctaagt caaaaaactt accacattta
    36181 gtacaataat ttctatgtac agaaccatga agttctagca catttttact tccagccatt
    36241 tgatgaagtc catcaatatt ttgagttatg acagctttaa gcttacccat ttcctcgagt
    36301 tttgctaaag ctatatgagc attgttgggt tttgcattag gatatattaa tttatcctta
    36361 taaaattcaa aaaattcttc tggatatcta acaaaaaaag tatgtgaaac taattgttca
    36421 gcagtaaatt gtttattgag tttttggcta aataatccag tagaacttct aaagtctgga
    36481 atattagatt cagttgaaac acctgctcca ccaaaaaata caatattatt gtgattagct
    36541 attaagtctt ttaagctatt tgcatccata gttacacctc caaattatta tatataaatt
    36601 gtactacata ttaggtcttt tatgttttat taatttaaat aaatatgtag tttaataagt
    36661 taaataaagg gaatataaat aaaatgtata cagagtataa atagacaata tttactaaaa
    36721 taacaagttg tcgtataatt atattaaaat tgttttagtg tataagaagg gcgggggaag
    36781 tacgtgaaga aaaaagcagc tttagcaaca ttggctatgt tacccttagg tgtggtaaat
    36841 gcacatgctg atggagatat aggtatagtg actataaatt atttaaatgt aagaaatgag
    36901 ccaactgccg aaagtagcat agcctttgtt gctaaaaaag atgataaagt tttgattaaa
    36961 gactcttcta atggatggta taaaataaaa gctgaatctg gacaagaagg ttgggcttca
    37021 tcaaaatata tagcaaaatc aaatagtgac tctttaagaa cgtctacaaa taaagaaaaa
    37081 caagttattt caaatagttt aaatatgaga aatggagcag gtacaagtta cagagtaata
    37141 actgtactaa aaaaaggtca aaaagtagaa gtaatatcag agagtaatgg atggtctaaa
    37201 atcaaatatg atggaagact aggatatgta tctagctctt atttaggaga cgtttcaaat
    37261 tcaactaata aatcaaaaac taaacaagtg aatacaactt cactaaatgt aaggagtgga
    37321 ccaaatacaa gttatggttt gttaggcaag ttgccaaaag gaagtaaagt agaagtaata
    37381 tcagaaagta atggatggtc caaaataaag tataatggaa aagatgccta tatatctagt
    37441 atgtacttat cggatgtaag tcaaagtaat tcagacaatt ctagtcaaag caatgataaa
    37501 aagaatactg ataaggttgt aaatacagct tctttaaatg taagaagtgg accaggttct
    37561 acatatagta agttgggaaa agtttataaa ggaagtaaag taactgtact atcagaaagt
    37621 agtggatggg ctaagattaa ttttaacaat aaagaagcat ttgtagtagg caattattta
    37681 tctacttcag cagatacttc aaataataac tcaaatagta actctgataa cagttctaat
    37741 agtaatggca ataattcttc atcatcaggt caagtaaatg gtatgtcagg tataagtggg
    37801 gctaaaattg attataaatc tttgagttat actttagaat ctcatataag taaacaagtt
    37861 gagaaagcag catcaggagg aaatgtgata gctccaagta ataggaaaag cactccaagt
    37921 cctgaattta gtactttttc agctcaaaga acaagctcat ttgtaaatgc aagttcaagt
    37981 gatatagaat attatttaaa tcctaaaaat tttacaaata ctactaaagg tatgatgcag
    38041 tttttaaaga ttaacagtta tagagatggt atttcagaat caagtcttaa ctcgtatctt
    38101 aatggtttat cttctagtgt atttaaaaac cagggagcag cctttataaa tgctgccaag
    38161 aagtataata tagatgttgt atatctagta tctcatgcta tgtgggaaac tgcttatggt
    38221 aagtctacac ttgcccaagg tcaaacatta acatcttata agggacaacc tcttagtaag
    38281 ccagttaaag tatacaattt ttttggtata ggagcaatag ataaaagtgc taatgtttca
    38341 ggtgcagaag cagcgtattc caatggctgg actagtgtag aagctacaat agatggctct
    38401 gcaaagtgga tatcacaaaa ttacgtaaat agttctaagt acaatcagaa tactatctac
    38461 aagatgaagt ggaattatga ttatacatgg catcagtatg caactgatgt aaactgggct
    38521 aatggaattt ctggaattat ggaaaattta attggtcttt atggaggagg aagtagtttg
    38581 gtttttgaag ttcctcaata taagtaatac ataaataaaa aatatagact atgggtagtt
    38641 ttcattttat ttaaattatc tatagtcttt ttgtatataa tttataatct gtaaatttaa
    38701 tgttaacaaa acttttatgt tataatatta attctaagta aatataagtg agtaataggt
    38761 agaaatagat aaaaagtggt aataatatat ataaagataa ttttgagaaa ggaggatata
    38821 tatggaaagt attaaggaat atttggataa gttagaaatt aataatagtg gtcttggaaa
    38881 gcaacttaaa gaagtttata taaatagagt agtttatttt aaggaagata agatagttta
    38941 tttttatcta acttctaaag atattgtttc tcatgaactt ttagataaat ttaaagaaga
    39001 attgatgtat aaactcgatt attttaaaga tatgaggatg aagataagat ttactggtct
    39061 tgaaagaaaa tccaataaag atgttataaa aaaatattgg aataatatat tgtatatatt
    39121 aaagtatctt tgtccatcta ttgcagggtg gtataaacaa gtagaatttc tttgtttaga
    39181 agaagaatta aaaataaaac ttccaaaagg tatattttat gagcgattaa tgaaaaaaaa
    39241 tgtaacttat gtacttaaaa ccgtgcttag cgaagagttg ggattagatt taaatataac
    39301 aatagaaaaa gctgttgatg aaaaggtaaa taaagaaaga ctcataagaa tcaatgatag
    39361 agaaatggaa gaaaaaatta gggctttaga aattggaaaa gttaataact gtgaagaaaa
    39421 tgaagaggaa agctatgtca ttaagtctga tgtagatgaa aatttaatct atggagataa
    39481 tgcaaatgct atgattgaaa atatagttga attaaatgca tcttctggaa cagttgcagt
    39541 tgttggagat atatttgatg ttgacactaa agaacttaaa aacggaaaaa tacttatgat
    39601 agcttcaatt acagattata ctagctcaat aagctgtaaa ctttttttaa cagatacaaa
    39661 taaggatgga gtacttgaaa atgttaaaaa gggagcatac ttaaaaatca agggtgacat
    39721 agtttatgat acatatcaaa gagaaattag tatgatgatt agtgggataa gaaaagaaac
    39781 tagaatagag agagaagata aatcagagga aaaaagggta gaactccatg cacatacaca
    39841 aatgtcttct atggatgcta tttgttctgt taaaaagctt gtagagcgtg cagccaaatg
    39901 gggtcattct gctattgcta taacagacca tggtgttgta caaggatttc cagatgctat
    39961 gaatgcaggt aagtcaaata atataaaaat actttatgga gtagaagggt acttggttga
    40021 agatgattct ctcattatag aggatgcaaa tgataaagaa ctttcgcaga catttgttgt
    40081 atttgacata gagacaactg gtttttctaa tacaaatgat aaaataactg agattggtgc
    40141 tgtaaaaata gaaaacttta agattgtaga tagatttagt gagttaataa atcctgaaaa
    40201 agatatttcc tataagatac aggaattgac aggaataact aatgaattaa ttaaagacaa
    40261 gccaaccatt gaagaaattc ttcctaagtt tatggaattt gttggagata gtgtattagt
    40321 agcacataat gcagagtttg ataccggatt tatatcgcaa aaatgtaggg aacaaggctt
    40381 gttatataat aacaaaaaag tagatacact tatgttagca agagtaatgt tgccaaactt
    40441 aaaaagacat agattaaatg tcgttgcaaa agaacttggt attcctcttt taaatcacca
    40501 tagagcagtg gatgatgctg aggctacagc acttatattt aataagtttt tgcagatgct
    40561 tacagaaaaa ggtgcaaaaa ctctaagtga tgtaaacaat atacttggaa agattgatta
    40621 cacaaagctt agcacaaatc atataacctt gatagcaaaa aattctatag gtataaaaaa
    40681 tttatataaa ataatttcag atgcacatgt aaatcatttt tttagagcac caagaatcct
    40741 tagaagtgta ttaaatgaat ataaagaagg attaataatt ggttcggctt gtgaagctgg
    40801 ggtggttttt caagctgtaa agaaaaacgt gtctgatgag gaaatgaaaa aaataataga
    40861 tttatacgat tatattgaag taatgcctat agataataat agatttatga ttgataaagg
    40921 agaagtaaag gatgaagaag agttaaggga gttgaatagg aaattaattg atacagccaa
    40981 aaaatttgac aaaattccag tagctactgg ggatgttcat tttttagaca aacatgaagc
    41041 tgtcttaaga aaagttctta aatattctca aggttttaaa gtagatgaag aagaaactta
    41101 tttacatttt agaacaacag atgaaatgtt ggaagagttt agttatcttg gagaagaatt
    41161 ggcatatgaa gttgtagttg aaaatagtaa tctgattgcc gatatggttg aagatataaa
    41221 acctatacca gatgatactt atcctccaat tatagagggc tcagatgtgg agttaagaga
    41281 aatgtgttac aaaaaagcca aacgaatcta tggagaccca ataccagaaa tagttcaaaa
    41341 aagactggat agagaattaa attcaataat aagcaatggg tatgcagtta tgtatattat
    41401 agctcaaaaa ctggttgcaa aatctctttc agatggatat ttagttggtt caagaggctc
    41461 agttggctca tcttttgcag ctactatgag tgatataaca gaagttaatc ctcttccagc
    41521 acattatatt tgtgaaaacg aagactgtaa atattcattc ttttatgaaa taggtgaatg
    41581 gggctcaggt gttgatttac cagacaagga ttgtccaaag tgtggaagaa aacttgctaa
    41641 aaacggtcat gatattccat ttgaggtttt cttaggtttt gaaggtgata aggaaccaga
    41701 tatagactta aatttctcag gaaattatca gccaacaatt cataaataca cagaagaact
    41761 atttggagag gggtatgtat atagggctgg gactataggt actgtagcag aaaaaacagc
    41821 ttttggatac gcaagaaaat atgtggaaga aaataatata agtgtaccaa atgcagaagt
    41881 acttaggctt tcaaatggat gtactggagt aaaaagaacc tcaggacaac atcctggtgg
    41941 agttatggtt ataccagact ataaagatgt atacgatttt acacctatac agtatccagc
    42001 aaatgatact tcttgtggtg ttataactac tcactttgat tatcattcaa taagtggtag
    42061 aatacttaaa cttgatatac ttggacacga cggtcctacc attataagga tgcttgaaga
    42121 tatgactgga ataaatataa cagaaattcc tttagatgat aaagagacta tgtctctatt
    42181 tacatctaca gaagcattag gtgttacacc agaagaaata aactgtccta taggttgcct
    42241 tgctgtacca gagtttggaa ctaagtttgt acgacagatg ttacttgaca ccaaacccac
    42301 tacatttgca gaacttgtgc gtatatctgg tctctctcat gggacagatg tttgggtaaa
    42361 taatgcacaa gacttagtta gagaagatat agttggatta aaagatgtta tatctacacg
    42421 agatgacata atgaactatt taatatttaa aggattacct cccaaaatga gttttacaat
    42481 aatggagagc gtaagaaaag gtaaaggtct aaaaccagaa cacattgaag aaatgaaaaa
    42541 aaataatgtt ccagaatggt atataggctc atgtaaaaag attaagtaca tgttcccaaa
    42601 agcacatgct gttgcttatg taatgacatc atttagaatt gcatattgta aagttcacta
    42661 tccagaagca ttttatgcga cttattttac gacaaaagtt gaggattttg atgcagattt
    42721 aattgttaaa ggattagatg ctataaaatc aaaaataaat gaaattgagt cactgggaaa
    42781 tgatgcaacc acaaaagaaa aaggtatgtt gaccgtttta gaagttgctc ttgaaatgta
    42841 tgctagagga ataaaattat taccagttga tatatataaa tcagatgcaa ctgagtttat
    42901 agttgtagga gaaaaaacat tacttccacc aatggcagca atacaagggc ttggaggaaa
    42961 tgcggctata aacatccaaa atgaaagaaa aaatggtgaa tttatatcaa aagaagattt
    43021 gagaaagaga acaaaaatat caaaaacggt aatagaaact cttactaatc atggttcact
    43081 tgagaatatg agtgagagaa atcaattgtc attgttttaa gttagcccaa aaagttgcaa
    43141 ataaaagaat tttatgatat catagtatag aaaatagtat actttgcaga aactaagagt
    43201 gagacagctg ttctcactct tttattttaa atatattatt aaaaagttgg tttattttta
    43261 ttaaatatat tttatttaag attatataaa ctaatcattt acaattgaat aggaggaact
    43321 ttaatgaaaa aaaatttaga agctacaatt gaggagattg taactaaaat aacggatgaa
    43381 catggatttg aaatggttga tgttgaatat gtaaaagaag caggagaata ctacttaaga
    43441 gtatacatag ataaagaaga aggaatatct ttaaatgaat gtgaattagt tagtagagaa
    43501 ttaagtccaa tactagatga aaaagaccct ataaaagaaa attacttttt agaggtatct
    43561 tctccaggac ttgatagagc attaaaaaaa gacagagatt ttgttagata tcaaggtaga
    43621 gatgtagatt taaaattata taaaccatta aatggatgta agcaatttga aggtgaactt
    43681 gtaggattaa cagaagataa taatataaaa atcattgcaa atggtaagga aatggaattt
    43741 aatagaaaag atgttgctat tgtcagatta gcaataaagt tttagtggta tattaaatag
    43801 ggaggatata aaaaatgaat catgaattta tggaagcatt agatgaatta gtagttgaca
    43861 gaggtataga taaagaaata cttatagata ctatagaaca agctctttta actgcataca
    43921 agaaaaactt tggttcagca caaaatgtta gagttgaatt tgatagagac aagggagata
    43981 taaaagtatt ttctcaaaga gttgttgttg atgaatcaga tttatatgat actttcttag
    44041 aaatagagtt agcggatgct agagagataa accctaatta tgaattaggt gatattatag
    44101 aacatgaagt tacacctaaa aactttggaa gaattgcagc tcaaacagca aagcaagtag
    44161 ttgttcaaag aataagggaa gctgagagag aaatagtgta taatgaattt atagaaaaag
    44221 aaaatgaaat agttacagga gaggttgcta gagcaaataa aaatgttgtt tatgtaaatc
    44281 ttggaagaat agaaggtata atgactcaaa gtgaacaaat accatttgag aattataaag
    44341 caggtcaaaa aataaaattc tatatacttg aagttaaaaa gactaacaaa ggacctcaaa
    44401 tagttgtatc aagaagtcat ccgggtctag taaagagatt gtttgaagaa gaagttcctg
    44461 aaatatatga aggaatagtt caaataaaat ctgtagcaag agaagcaggg tcaagaacta
    44521 aaatggcagt aaaatctata gatgaaaaaa tagacccaat tggagcttgt gttggaccaa
    44581 aaggacttag agtaaaaaat atagtggatg aacttggcga tgagaaaata gatataataa
    44641 aatatagtga agacccagca gagtttatat cagcagcact tagcccatca aaagttgtta
    44701 gagtagatat agatgaagat gaaaagtctg ccttagtaat cgttccagat tatcagttat
    44761 cgcttgctat aggtaaagaa ggtcaaaatg caagactagc agcaaaattg actaactgga
    44821 aaatagatat taagagcgaa tctcaagcag aacaagaaat gttaaatctt gaaaattcca
    44881 aagaaataga agctgaggat gttgaagctg aatttatcga ccaagaagag ttaggtactt
    44941 cagaggataa ttttagtgaa gaagagtaag gagggatgat tttgaaaaaa gtaaaaaaag
    45001 tgcctcaaag aaaatgcata gcatgtcaag atagagactc taaaaaagag cttataagaa
    45061 tagttaaaaa taaagaagga aaaatatttt tagacccaac aggaagagcc aatggaagag
    45121 gagcatatgt atgtaaaagc tctgaatgtc taaaaaaagc tataaagagt aaagcattaa
    45181 ataaagcttt taaaatagat gttccagatg aagtctatga taatctactt atggagttag
    45241 aagaatatga aaaataataa agaaaaagta ctttcgttct tagggctcgc aactcgtgca
    45301 gggaaaatag tgtctggtga tgattcaact ttgcttgacc ttaaaaaagg aaaagtaaat
    45361 ttgattttaa tcgcagaaga tgcatcaaat aatactaaaa aactttttaa agataaatca
    45421 acttttagaa atattccata tctatttttt tcaacaaaag aggaaatagg atttgcaata
    45481 ggaaaatctc caagagcagt tgtaggaata aaagatgaaa atttttctaa aaagataatt
    45541 gaactaatag aaatttaaaa taaaataggg ggtgatcttg tgtcaaaaac aagagtatac
    45601 caaatagcag aagaattaaa tatatcaaat gaagagctta taaataagtt agctgaatta
    45661 gatataaatg taactgacaa agattcagta ttagaaggtg aagagttaga attagcactt
    45721 gaaatgttag gtgaagattt atctcaagaa aatggaaatg ttatagaaat agatggaaag
    45781 ttgacagtac aagtattagc aacgaaatta gataaatctc catcagaaat aattatgaag
    45841 cttatgaaaa tgggaactat ggcaacaata aatcaagaga taagttttga gattgctgct
    45901 ttggctgcca aagactatgg atttgaatta acagtagctg aaagtgatga tacagaagct
    45961 ttagaaatag aagctttaat ggaaattgaa gaagataaag aagaggattt aaaacctaga
    46021 ccaccagttg ttacagttat gggacatgtt gaccatggta agacatcttt actagatgca
    46081 ataagaaaaa ctgatgtgat ttcaggtgaa gctggaggaa taactcagca tataggtgct
    46141 tcagaagtta aaatcaatgg acacaaaata gtatttttag atactccagg acatgaggct
    46201 ttcacatcaa tgagagcaag aggagcacaa gttacagata tagcaatatt agtagttgca
    46261 gcagatgatg gtataatgcc tcaaacagtt gaagctataa accatgctaa agcagcaggt
    46321 gtacctttaa tagttgctat taataaaata gataaaccag gtgcaaaccc agataaggtt
    46381 aaacaggaat tagcagacca aggattatta gttgaagact ggggtggaga agtaatagca
    46441 gttccagtat cagctaagaa aaaagaagga atagatacac ttttagaaat ggtattattg
    46501 gttgctgaaa tggaagagtt aagagctaat ccaaataaaa gagcagtagg aactgttata
    46561 gaagctgagc ttgataaagg aagagggcca gttgcaacag tacttgttca aggtggtact
    46621 ttaacagtag gtgacccaat cgttgcaggt gttgcatgtg gtaaagttag agcaatgata
    46681 aatgcaaaag gtaagagagt aaaaacagca ggaccatcta cagcagttga gatattaggt
    46741 ctatcagaag ttccacaagg aggagaccaa tttgtcgaag ttccaactga taagattgct
    46801 agaagtgtag cagcaagaag acaacaaata gttagagatg aaatgttaaa atcaactcaa
    46861 agattatcac tagatgctct atttagtcaa atgagtgaag gttctataaa agaccttaat
    46921 atagttataa aagcagatgt acaaggttca gtacaggcag ttaaacaatc tttagaaaaa
    46981 ttatcaaatg aagaagttca agttaaagta atacatggtg gtgttggagc tgtaacagaa
    47041 tcagacatat tacttgcagc agcttctaac gcaataataa taggattcaa tgtaagacca
    47101 gttccaggtg cagaatctct aggagaaaaa gaaaatgttg atataagaac atatactata
    47161 atatacaagg caatagaaga tatacaagcg gctatgactg gtatgttaga ccctgaatat
    47221 gttgatgaag aaacaggaaa agctgaaata agagaaatat ataaaatatc tggtgttgga
    47281 acagtagctg gatgttatgt gactaatggt aagatattta gaaattgtaa agtaagatta
    47341 gttagagata gcataataat acatgaaggt gagcttgcag cacttaagag atttaaagat
    47401 gatgtaaaag aagttaattc tggatacgaa tgtggtatga gttttgtaaa ttataacgac
    47461 ataaaagaag gcgatatagt agaagcttat ataactaaag aagtagaaag aaaattataa
    47521 attcgtatca aagaaaatca gttagtaaag aaggtcgatg gtatggcatc atataataga
    47581 acaagaagga tagcagaaga aataagaaaa gtcgtaagta ctatgcttat aaatggagta
    47641 aaagacccta gaataacatc tatggtgagt gtaactgatg tagaagttac aaatgaccta
    47701 agatatgctt atgtatatgt aagtattcta ggtggagatg aagaatccac actaacaggt
    47761 cttaaaagtg ctggaggata tataagaaga gaagtaggaa aaaatataaa acttagatat
    47821 attcctgaga tagtatttaa acttgatgat tcaatagaaa aaggaatgta tatggataac
    47881 cttataaaaa gagttaatga aaaaaatgct caacaaaatg aggatgaaaa ttatgatgaa
    47941 taatatggat agtattataa actatattta tgagggtgac tattttatag ttacctctca
    48001 tatcagtcca gatggagaca atgttggttc tacactttca atgtattata cattgaagaa
    48061 tttaggaaaa aatgtatatt atgtacttga tgatgaagct ccattaaatt taaggttttt
    48121 ggttgaaggt gtaaaaatct ataagtctaa tgaatttaat atgaagaact attctttaat
    48181 tgcacttgat tgtggggata agatgagaat atgtgtgagt gatgaaatca aaaataatgc
    48241 atcaaaatta atctgtattg accatcatgc tagtaatgat tcatatggag atttaaatta
    48301 tgtagatatt gatgcatctt ctacatgtga actagtttat aatttattgg ttagatttca
    48361 gcaaacaaaa gatattaata ttataaatga agatatagca acatgtttat atacaggtct
    48421 tgtaactgat actggaaact tttcgtattc aaatgtacat gcaagtagtt ttgaaatggc
    48481 aaaaaattta cttgttttag gtgcacaaaa aaatactata atacaaaata tatatcaaag
    48541 caattcttct gattattata aattattagg agaagctcta aaaggtttgg agatatttga
    48601 ttcaaaggta tctagtatag ttttaactca agacatgatg aatagaaata atattagctt
    48661 taatgatgtt gatggaataa caacatatac aagagatata aaaggtatag aagttggaat
    48721 tttatttaaa gagaaaaaac aaaatgaaat taaagtgagt tttaggtcaa aaaattatgt
    48781 tgatgtaagt gaaatagcca agttatttgg cggtggtggt catgtaaggg ctgctggatg
    48841 tactattaga gatagtattg ataatgcaaa gaaaatggta ctagaggcag tgttaaaatc
    48901 aatttaaggt gacagtaaaa tgaataagat tataagtata ttgaaaccca caggtatgac
    48961 atctcatgat gttgtaagta gagtgagaaa gattttaaat ataaaaaaag ttggtcatac
    49021 aggtactctt gacccagatg cttcaggtgt tttaccaatc tgtataggga aagctacaaa
    49081 agtatgtgaa gttattttaa acaaagataa atcgtatata tgtgagctta ctttaggtat
    49141 aagtacagat acttatgatg cttcaggtga aattttaaaa aaagttgatg attttaaatt
    49201 tagtaatgaa gacatagaaa gagcttttga tacacaaaga ggagagataa atcaacttcc
    49261 tccaatttat tcagctttaa aggtaaatgg aaagagaatg tgtgatttag taagaagtgg
    49321 tagacaatct gaaattactt taaaaacaag aagagtaaat ataaaagaca taaaaatact
    49381 aagtataaaa ggtaataaag taatgtttta tgttgagtgt tctaaaggga cttatgtgag
    49441 aagtatttgc catgatatag gagaatatct gggttgtgga gctcatatgt cgtttttgaa
    49501 tagaacatct tctggtaaat ttgacttaga taattcaata acattagaag aattagaatt
    49561 attttatgaa aataaaactt tagacaaata cttatatgat atagattatg tccttgattc
    49621 gtttaattat gttgtattaa accctaatgc tataaaatat tatagtaatg gtggctcaat
    49681 agatgataaa aggtttttaa aaaataattt cgataaagat gatgaagttg taagagtata
    49741 cagtacagat aatttcttag gattaggaaa gttatcaaaa cacaataata caataagtgt
    49801 taaaagtgat aagatgttca tataaccaat gtatttaaaa aaacaacaaa agagatagta
    49861 ctataataac ttctatagtc tatctctttt acttatgcta taatagtttg tgaataagtt
    49921 aagagtaaat tatattttag cggggaacat ttattatggt gattataaaa tctataaaag
    49981 aaatagcaaa cataaaagaa agtgtcatta ccataggaaa ttttgatggt gtacacaaag
    50041 gacatcaagt tcttatagga aaaacagttg aatatgcaaa aaaagaaaat atacagagta
    50101 ttgtatttac atttgcaaat catcctgtaa attactttag accaaactca acaaaaaata
    50161 ttataagcaa tgatgataaa ataaaattat tagaagattt gggaatagat atagttgtaa
    50221 tcataccatt tgatgagtat atgactaaaa tacctgcaaa ggatttcgta gatgagatac
    50281 tagtaaaaaa attaaaagct aaaaagatag taattggaca tgactttaca tttgcaaggg
    50341 caaaagaagg caatgttaat ctattaaaaa gcttagaaca tgaatttggt tttgaggtag
    50401 aagtaataaa acccatcaaa atcaatgatg taagagtgag tagtacttat attagaagtt
    50461 tagtatccca gggtgatatg gctaatgtca aggaatattt gggtagaaat tacaagttag
    50521 aaggttgtgt tatacattct aaacaccttg gaagaactat aggattccca acggcaaata
    50581 ttgaccttaa gaataatatg ctcgttccaa agagaggtat ttatgcttct attgtacata
    50641 ttggagatga gacttatttt ggtgctacaa atgtaggcta taacccaaca gttaatggaa
    50701 aaactttgtc tattgaaaca aatattttag agtttgatag agatatttat ggggaaaata
    50761 taattgttga atttttggaa agaataagag atgaaagaaa gtttaattca atagatgact
    50821 taaaaaatca attgtataat gatacaaatt atgtgtatga aaactatgtt tgcaaaaaca
    50881 agtaatgtat gataaaatga ttaaggaaaa taaccattac tcggcatttg aatactccaa
    50941 tcaatgctta gtaataggcg tttctaaaaa aatacggagg taatctaaat gataaacaaa
    51001 tcagaaataa taaaagaata tggaagagct gaaggggata ctggttctcc agaagtacaa
    51061 atagctttat taacagctag aataaacgag ttaaatggac atttaaaaac tcataagaaa
    51121 gaccaccatt caagaagagg actattaaaa atggttggta gaagaagaaa cttattagct
    51181 tacttaaagg aaaaagatct tgaaggatat agagcattaa tagctaaatt aggattaaga
    51241 aagtaatttc attctttata aaaaagcagg tatagtgtat acctgctttt ttatagttaa
    51301 ttcattataa agtaaaatag ttaataataa aagtagttta tatgttataa aaaattataa
    51361 aatgaataat attgggtaag aatattaaaa tagtatacaa atacataatc aatttaaaag
    51421 gaaagacagg ataaatatag aaattttata acaggataat tagttggaat aaggaggata
    51481 ttattttgaa aataaagtta gtatgcgata gtttgtgcga tattccagat gaaatatcag
    51541 aaaaagatta tatagagata gttccactta cagttatttt taatgatagg gaatatatag
    51601 aaggagtaga tataaagaaa gaagagtttt ataaaaaagt aaaagaaatc aagcaaatac
    51661 ctaaaacttc acaagctaca tatatggagt ttaaagaagt gtttgataag ttcataacag
    51721 aaggttatca tataatctgt atgacagggg ctgcaaacgc atcaggaact tttcaaagtg
    51781 ctatgatagc taaaaatgat gtaaatgaaa atgaaaaaat acatatattt gatactagaa
    51841 atttatctct aggtagtgga caatatgtta taaaggcatg tgaacttctt gaagaaggac
    51901 tgggatttga agaaattata gatgaattag aaaatactcg cagtagtgta agattacttt
    51961 ttgcaccata tacacttgat tttttaaagc agagtggaag agttccagtt gcaactgctt
    52021 tgataggaaa tatgttaaat ataaagccta tatttttctt tgataatggg gaagctaagt
    52081 tggtaaataa ggttagagga ataaagaata tagcaagtaa actggtcgat ataattttag
    52141 aaatgaatga agggtcttta gaaggaaaaa tcgtcacaat aggttgtgga gataatttac
    52201 acgattgtga aatattaaaa gatgaagtaa ataaaagaat aaaagcaaga agagtattat
    52261 ttactcgagg aggagtttct atttgctctc atacagggcc agatatattg gctataagtt
    52321 gttctaatta gtagtaatat aaggtttttg catatatatg tgaagataat tttatattgt
    52381 taatttatag tatttttgat aatatagtaa atagagtgat atacgtgttt tttaataaga
    52441 ggaggacaag cattaatgtt tgaacataaa atatttaaaa tggattttgc aggaagagaa
    52501 ctttctgtag aaataggaaa gatttgtgaa atggctagtg gtagttgtat agttcgatac
    52561 agtgacagta tggtaatggt aaatacaact aaatcagcca aacctagaga cggaatagac
    52621 ttttttcctt taagtgtgga ttatgaagaa aaattatact ctgtaggtaa gatacctgga
    52681 ggttttttaa agagagaagg gaagccttca gaaaaagcta tacttacatc aagattgata
    52741 gatagaccaa taagaccttt attcccaaaa ggatttagaa atgatgttca ggtagttgca
    52801 acagttctat cagtagacca agattgtaca ccagatatag tagcaatgat aggttcttca
    52861 atagcccttt ctatatcaga cataccattt aatggaccaa caggttcagt atgtgttggc
    52921 cttgtagatg gtgcatttgt tgtaaatcca aacgcagagc aaagagaaaa aagttcaatg
    52981 catctagttg tttcaggtac aaaggaagca ataatgatgg ttgaagccgg agctgatgaa
    53041 gttccagatg aggtaatgct tgatgctatt ttatttgctc accaagaaat aaaaaaaata
    53101 gttgagttta tagaaggtat agttgcagaa gttggaaaag aaaaaatgcc tgttgaatta
    53161 taccatgcag gagaagaaat aactcaattg gttcgcgaat ttgcaactga taagatgaaa
    53221 aaagcagttc agacttttga aaagttagag agaatggaaa atatggatag agttaaagaa
    53281 gaaactttag ctcactttga agaaacttta gaagattttg aagattttgt tggagatata
    53341 gaagaagtat tacaagatat aataaaagaa gaagtaagaa aacttatagt acatgaaaat
    53401 gtaagaccag ataatagaaa acttgaagaa ataagaccta tatggtgtga aactggcatg
    53461 atacctagag ctcatggttc agccatattt actagaggac aaactcaagt acttaatgtt
    53521 gctactttag gtgctttagg agatgttcaa aaattagatg ggttagatga agaagaaaac
    53581 aagagatata tgcatcatta taatttcccc gcatatagtg ttggagaagc tagaccatca
    53641 agaggaccag gaagaagaga aataggacat ggtgctttag cagaaagagc tttattacca
    53701 gtaataccat ctcaagaaga atttccttat gctattagat tagtttctga agtattaagt
    53761 tcaaatggtt ctacatctca agcaagtgta tgtggctcaa cattatcttt acttgatgca
    53821 ggtgtaccta taaaagatat ggttgcaggt atagcaatgg gacttataaa gcatgatggt
    53881 aaggttgcag tgctttcaga tatacaaggt atggaagacc atttaggcga tatggacttt
    53941 aaagtagctg gaacagagta tggtataaca gcaatacaaa tggatataaa aattgatggt
    54001 atagataaag aaattctaca acgtgcttta aaacaagcta aagagggtag aatccatata
    54061 ctaggagaga tgagaaaaac tatatctcag ccaaaaccag aactttcacc atatgcacct
    54121 aagattgtta aaatgcaaat aaatcctgac aagataaaag atgtaatagg gcctggtgga
    54181 aagataataa ctaagataat agacgaaact ggagttaaga tagatattga acaaactggg
    54241 gaagtattta tatcaggtat tgagattgat atgattaaga aagctcaaga acttataaat
    54301 aatatagtag ttgaaccaga agtaggaaaa acttataaag gtaaggtaag tagaataatg
    54361 aactttggag catttgttga aatacttcca ggaaaagaag gattacttca tatatctcat
    54421 atagctcatg aaagagttgc caaagttgaa gatgtattga atatagggga tgaagttgaa
    54481 gttaaagtta cagagattga tgaaaaagga agagttaatt tatcaagaaa agttctttta
    54541 ccaaaaccag aacataaaaa taaataactt attatataat taaaagcaaa gtcttaggat
    54601 tttgcttttt taaatacaaa aaagtagata attttaaaat gatagttatg gatggcttat
    54661 agttcttttt ctgtaaggct ttactcttat aaagaagcta tttaaaagct gattaataga
    54721 aagctttatt gatatatata gaatcactta agttatattt agtgcataaa cagagcttat
    54781 ttttaatagt attaaataag aaacaatttg tacataatta gggggagttt taatgataat
    54841 gatggtactt aataagagta agctcaaaaa aatcataata ctaattttag tagtgttatt
    54901 agtattatca ggaggggtaa tggtattaaa gttatttaac aacaagccag tatttaattt
    54961 atttaatggt gtagatttac catatgaaag aggtacaaag gataaaagtg gatatgttgc
    55021 attaacctgt aatatagacc ttggatggga aacagagtac gtagaatcca ttttagaaac
    55081 acttaaaaaa gaaaatgtaa agattacatt taatgtaact ggaaaatggg cagaaaagaa
    55141 taaggatgaa ttattaaaga taaaaaaaca aggacatgaa ataggtaatc atggatataa
    55201 acatttagat tattctacat tatcatatga agacaattat gagcaaatag agacctctaa
    55261 aaagataatt gaagaaatta taggtgaaaa aactaaattt tttcaagcac cagcaggttc
    55321 ctttggtcca gaaacagtaa aggctgctaa agcattaggg tatacttcta tcaaatggga
    55381 tgccgatact atagattgga aatacaaaga ccaaccagag gtaatcattg atagaatgaa
    55441 gaaaaaagac attaaagata gtagtataat cttaatgcat ccaaccaacg caacgacaaa
    55501 atgtatagat gatattatag ctattgtaag agaaaaagga ctaaaaccag ggaaacttag
    55561 tgatgtattt aagtagacaa agtataaata tttggttaag attgtataag ttgtagttga
    55621 attaattaaa agactgactt ataatgtaat acatataaaa aataaatata attaaaacat
    55681 agtgttttca ataatccaat atgaaacaaa gaataaattg ttaggagaat gtaatgtata
    55741 agacaaaaat actggaaaat gggcttacaa taattggtga agaaatacca tatctaaagt
    55801 caataacatt aggtatttgg ataaatgcag gctctaggat agaagaagca caggtgagtg
    55861 gaacttctca ttttatagag catatgatgt ttaaaggaac caaaaacaga acatcaaaag
    55921 agattgctag ttcaatagat aatttgggtg gtcaaataaa tgcatttaca agtaaagaat
    55981 gtacatgtta ttatgtaaaa ctaatagatg agcatataga tacgggaatt gatgtattga
    56041 gtgatatgat actaaattct aaatttgata aaaatgatat agataaggaa agacttataa
    56101 tattggaaga acttaagatg tatgaagact ctccagatga cttatcttat gatttattag
    56161 tggaaaatat atatgctaat gatggattag gaatgaatat aattgggaca aaagaatctc
    56221 tatacaatat aactagagaa tccatgttag aatacttaaa taagtattat attccaaata
    56281 acgctgttat ttctatagct ggaaacttta actttgatga tatggtggag aaaatcaaat
    56341 caaaatttgg acactgggaa aagaaaaatt tgagtataga cataagtgag gctaagttta
    56401 atccttgctt tatatcaaaa aataaagata ctgaacaggt gaacttagct atctgcctaa
    56461 aaggtattcc ttttgaaaat gatgaagaag tctactcaat ggcagtggta aataatattt
    56521 tcggtggaag tataagctca aggctttttc agaaaataag ggaagaaaaa ggtttagtat
    56581 attcaatata ctcatcacag acattatata ggaaatgtgg tgaattaggc atatttgcaa
    56641 gtatgagtac agaaaattta caagatgtat ataatttaat aaagaaagaa attgaaaata
    56701 taagagaaaa ctatcttaca gaaaaagaaa tatctgaaag caaggaacag ttaaaaggaa
    56761 attatatatt ggatttagaa agtactagca gtagaatgat gtctactggt aaatctatgt
    56821 tacttagcaa aaaagtaaaa actacagatg aaatacttga atgtataaat aatgttaata
    56881 ttaattcaat aaagaaggtt gttgataagg tattcaatat cgaaaatatt ggtacatgta
    56941 tagttggaag agatgtagaa aagattctac acttggatta atattgaaaa aagtaaatct
    57001 ctggaggtat aaaatatgaa tttatctgaa atagcaggta aggaaatagt taacttggtc
    57061 acaggagaaa gattgggggt cattggagaa tgtgacctta tactagatga aactacaggc
    57121 ggtatattag cgcttctaat tccaaaggaa aaaggatttt ttgccagaag aaaagataag
    57181 tcatttttag aagtaccatg gagaaatgta aaaaaaatag gtaatgacat gataattata
    57241 gaatatgagg gatatattta ggaggaaaaa atgagtattt tagtgcaaaa gtttggtggg
    57301 acatcagtag aatcatatga aaaaatgaat gaagtatgta agatagtaaa agcatacaaa
    57361 aaaaatgatg aagaattaca attagtatta gtagtttcag caatgggaag aaaaggagct
    57421 ccatatgcta cagatacact tataaatttg tgtagtgcag taaatgatga gccaagtaaa
    57481 agagaacttg atttaataat gtcttgtgga gaaataatat caggaacaat tcttgctaat
    57541 ttattgaatg cacaggggat agattctgtt ttctttactg gtcagcaagc aggtataatt
    57601 acaagtgatg aatattctaa tgcaaagata aagtatataa atcctaaaaa ggtaaaaaga
    57661 gctttagatg atggtaaggt tgtagttata gctggattcc agggtgtaac agatgatgga
    57721 gaaataacta ctcttggaag aggaggaagc gatacatcag cagttgctat tggtaaagct
    57781 ttagaatgtg aaacagttga aatttacact gatgtggatg gtataatgac tgcagaccct
    57841 agagtagaac caaacgctaa agtattgagt tttatagatt atgaggaagt attccaaatg
    57901 gctgacaaag gtgcaaaagt aatacatcca agagcagtac agttggctaa aagtggaaat
    57961 ataactttag ccataaagaa tacaatgaat ccaacttttg aaggtacaaa aataggttct
    58021 ttatgtagac acttagaaga gaatatagaa tatgaacaag aaagagattt taaggttgct
    58081 gttgcaaata aagatagtgt agcacaagtt aagattaaat cagcagaaga agtgtttaca
    58141 gaagtattaa atgagataga aaagaaactt ataactatag atatgataaa tttctttata
    58201 tcagaaaaag catttgttgt agaagatgct gatataaaat cattaaagga aatactagat
    58261 aaatttgaat tagattatga agtaaataga gactgtgcta aggtaacatt gatatgttca
    58321 agaattgctg atgaaatgtc tggtatcatg tcaaaggtag ttagaggatt atcaaaagca
    58381 ggggtatctc ttttacaaac atcagactca aatatgacta tatcttgttt agttagtgaa
    58441 gaagatatgc atacagcagt acatgctata caccaacaat tttatttaaa ataaactgta
    58501 taatacaatt attttttaat caaatttaag aatttcatat atatcttaat atatttgatt
    58561 tatttggtaa aactaaatta aatataattt tgataggagt ttttttatga aagattatag
    58621 agaagataat gaaagatttg ataaagataa ttgtagtgaa gaatctagtt gcgaagagag
    58681 agaatgtata aagcaatttg gtaccaatga aatgccacct caacctccta aagatattca
    58741 atgtataact ataattggag aaattgaggg acattttata ggaaatcctc aaaaaaaagc
    58801 tacaaagtac gagcatataa ttccaatgct atactcaatt gaggaaagca atgatgttaa
    58861 aggagtgtta gttgttttaa acactgttgg aggagatatt gaagcaggtt tagctattgc
    58921 agaactttta aacagtacat ctaaaaaagt agttactctt gttctaggag gaagtcatag
    58981 tataggtgtt cctcttgcta cagcaggaga ttactcattt atagcaccaa cagcaaccat
    59041 gattatacat ccagtgcgaa ctactggtct tgtgattggt ataaatgaga cttttgaata
    59101 ttttaaaaag atgcaagata gaataattca atttataata agaacatcaa atatcaaaaa
    59161 agatgtactt gaaaaactta tgcatgaaaa agatgaactt gtaagtgatg ttggtagtgt
    59221 attaatagga aaagaagctg tggattatgg tcttatagat gaagttggag gactaaaaga
    59281 agcacttaaa aaattaagag agcttataaa agaatctgag gaagaaaaaa ataatgatta
    59341 aaatttgttt aaataataaa atttatgata taatgatgta ataaagtagg agtgttagtg
    59401 ttaaggcatt agcacttttt gctgtgaagg ggtaaaactt gctaaaaata aggagtgata
    59461 ttaatggcca cgaaaaagaa gaaaaaaaag aaggtaaata ccttaagttt taatgcagaa
    59521 tatcataatt taataacaat atttataggt gtatttttat tatatagttt aaattctaat
    59581 tctatgggat ggatacctgt gttaatgcaa aatcttttta agggactatt tggagggctt
    59641 tcaatagcaa taccatttat agtaattata acaggacttt taggattttt tgatggaaat
    59701 gaatatatat ataggctaag aaagactaaa ctatactaca taattatttt atttatattt
    59761 gtattttatg gattattaaa tgcaggaact cttcctgtag atagcccact aaaaggtaac
    59821 atgtttgatg atgttatgaa attgggtgta agtggacaag gtagtggttt gatagcaaca
    59881 actattgcgt attatatgag taaaatattt ggtatagcag gtggatggtt aataagtatc
    59941 ttcgcactta tactttctgt aatgtttata tttaatattt ctataaaaga tttggtgtca
    60001 aatgcaaaat caaaatctaa agcatctaaa gacagcaatt taacgtttaa agataaaatt
    60061 gcaaatatga aaaaatctgc aatagatatg atgactgatg aagtagatga tactactata
    60121 aataaaaaac caggtttctt taaagggtta atgtcaaaag gtcttaataa tgatgatgaa
    60181 gaagatgaat attttgaaga agaaaatagt gatggtgtag atgataaaac tataaaaata
    60241 gttggattta ataaggcaga agatgagtat ttagaaatat tagaaggaac tcaaagtatg
    60301 ccagagttag atgttttaaa agaattacaa aaagctacca atgaaaatcc agttgtagat
    60361 actaaaccag aaaaaaaagt tgatatagct aaatctaatt taaatataga aaaaactcaa
    60421 cctatgagta ttgtagcaga gccagttaat gaggattatt caaactataa aaagcctagc
    60481 attgagcttt taaataaagt aaataagaag tctgatgaaa atggtaaaaa gaaagtgttg
    60541 aaaaatgctt ctcttttaga aaagacacta tcagattttg gagtggaagc aaaaattaat
    60601 caagttacag ttggacctac tataacaaga tatgaaatac aaccaagtcc aggagtcaag
    60661 gtaagtaaaa ttgtaaattt aactgatgat atagctttaa gtttagcagc taaaagtata
    60721 agaattgaag ccccaattcc tggtaaaagt gcaattggaa tagaagttcc aaatgaagaa
    60781 gctcaaatgg ttggtgtgag agaagttcta gaaagtgaag aatttaataa ttttaactct
    60841 cctcttgcaa tgggacttgg aaaagacgta gcaggaaaaa ttataattgg agatataggt
    60901 aagatgccac acttactaat agctggttct acaggttcag gaaagagtgt gtgtgtaaat
    60961 acattaataa gtagtatttt atataaggca aatcctgatg aagtaaaatt actattaata
    61021 gacccaaaag tagttgaact tgcaaattat aatggaatac cacatctatt gataccagta
    61081 gttactgacc ctaaaaaagc tgcaaatgct ttaaattggg cagttacaga gatgaataga
    61141 aggtataaat tatttgcaga tgctcaagta aaagatgtaa caagttataa tgagaaagcg
    61201 gaagaaaaac ttcctaaaat agtaataata atagatgagt tagcagattt aatgatggca
    61261 agtgcaaatg atgtagaaga ttatatttgt agattagctc aaatggcaag agctgctgga
    61321 atgcacttga ttgtagcaac tcaaagacca tctgttgacg ttataacagg ggtaataaag
    61381 gctaatatac catctagaat agcatttgca gtatcatctc aaactgattc aagaacaatc
    61441 cttgatatgg gtggtgcaga gaagcttcta ggtaaaggtg atatgttatt ctatccactt
    61501 ggtgcagcaa aaccagttag acttcaaggg gcattcatat cagagagtga gtcggaaaaa
    61561 gtaattgatt ttgtaaaaag tcaggttaaa gatggaataa aatatgaaga agatataatt
    61621 gaaactatat ctaaggttaa tacttcaaag agtagtgatg aggatgagtt tttgagtgaa
    61681 gcgatagaat ttgtcgtgga aagtggtcaa gcatctgcct ctatgctaca gagaagattt
    61741 aaaataggat tcaatagagc agctagactt atagattcta tggaagaaag aggcataatt
    61801 ggagcaagtg agggaagtaa acctagaaag gtattaataa gcaaacagga tttgcagaat
    61861 ttagaaggtg aatagaattg tcagttataa aaatagaaaa agcacttaag ctagataaag
    61921 taatatttgt agatgtgaga actgaaggag aatatgaaga agaccatata ttgaatgcaa
    61981 tcaatatgcc tctttttaaa aataatgaac ataatgaagt tggaacaatt tataagatgc
    62041 agggaaaaca tgaagcaata caaaaagggt ttgactatgt atcgtataaa ttaaaggata
    62101 tatatttaca agcagcagaa cttgcattaa attatgataa tattgtcata tattgtgcaa
    62161 gaggcggaat gagaagtgga agtatagtaa atttattatc atcattaggt gtaaatgtat
    62221 atcaacttga aggtggatat aaggcttata gaaattttgt attagagtat ctaaggcatg
    62281 taatggatac aaaaaacttt gtatctgttc atggattgac tggtacagga aagactgatt
    62341 tgttacattt attagatgaa aaaggaatag atgtactgga tttagaaggc atagctaaaa
    62401 acagtggttc tacctttggt tttataactt ttgataaaaa gccaccatct caaaagaact
    62461 ttgaaacaaa actatttgaa agtgtatttt tttcgaaaga gaattatata tttgtcgaga
    62521 gtgaaagcaa acgagttgga agtgttacag ttccaaatga aattttcgag gcaatgacac
    62581 gagaaggaca tcatatatta ttagaatgtg atattgaaaa tagagttgat agactttgta
    62641 gagattatat ttatgataaa gatgaaggta atatattgat tttaaaagaa tgtataaaca
    62701 agtttagaaa aagattgggt aataaagtag tagatgatta tattgattta ttagaatcag
    62761 gtgaatataa agaacttgta aaaagatatc ttttagaata ctacgaccca ctttatatgc
    62821 attcagtaga gaaatataag tatagtaaga ttataaattt taatgataca caaaaagctt
    62881 tagaagatgt tatagactta tatgagagta tattggaagg agaaagtgaa tgttaaaaat
    62941 agcattggaa tcattaggat gctcaaaaaa tttagtagat gcagaaataa tgatgggaat
    63001 tttaaataac aaaggctata aattaattgg agattttgaa gaagctgatg taattatagt
    63061 aaatacatgt gggtttatag agtcagctaa acaagaatct atagatacga taataaactt
    63121 ttcagagtta aaaaaaactg gcaatttaaa gttgttgata gtaacagggt gtctagccca
    63181 aagatattct gaagaattaa aaactgaaat tcctgaaata gatgctatag taggaacagg
    63241 aagctatcaa aatatagata aaatacttaa agaattaagt gaaatgcatc aaattgtaag
    63301 ccttaatgat atagagtttg tatttaatga agatttacca agatatatat ctacacctag
    63361 ctatatggcc tatttaaaaa ttggagaagg atgctccaat aattgtactt attgtataat
    63421 accaaaacta agaggtaaat atagaagtag aaaatttgaa gatataatta aagaagctaa
    63481 gaaattagct gaatctggag taaaagaact tgtggttata gcacaagata cgacaaagta
    63541 tggttttgat ttatatggaa aagaaagatt atcagaactt ttagaagaac ttgcaaaaat
    63601 agatggattt aagtggataa gggttatgta ttcatatcca gaatctataa cagaggaatt
    63661 gatacaagtt ataaagaaat atgataatat atgtagttac tttgatatgc caatacagca
    63721 tgcaagtaac aatattttaa aattaatgaa tagaaaaact actaaagaag atatattaaa
    63781 taaaatcaat ctgataagaa gtaatatacc agatgctata cttagaacta cgataatagt
    63841 agggtttcct ggtgaaacag aagatgactt caaacaactt gtggactttg ttgaagaggt
    63901 taaatttgat agacttggtg catttgcata ttcaagagaa gaagatacac cagcagatag
    63961 attgccaaac catatagatg aagaagtaaa aatacaaaga agagatactt taatgatgat
    64021 tcaacaaaaa atatctgaag aacttaatga caaaaagata ggaaaaactt atgaggtgtt
    64081 aatagaagaa cagatagaag ataatgtata tacaggaaga acacaaggtg atgcggaaga
    64141 aattgacagt atagtgtatg taaaaagtgt agacaatctt gaagtaggag aatttgtaag
    64201 tgttcaaata aatgatgcaa tggaatacga tttaatggga gatgttttat atgaacttgc
    64261 ctaacaagtt aactttgttt agaatatttt taataccagt atttgtttta attatgttgc
    64321 tcaatgtacc aaataaattt ttaatagctt gtattatttt tataatagct tcaattactg
    64381 atgctttaga tggaaaaata gctagaaaat acaatctagt cactgacttt ggaaaattta
    64441 tggacccatt agcagataag ttattggtta tttcagcact tacatgtatg atagaagacc
    64501 atcttgtaag tagttggatg gtaattataa tagtggctag agagcttaca gttagcatat
    64561 taagagctat agctgctgct gatggaaaag taattgctgc tggaaacagt ggaaaactta
    64621 aaacgataac tcaaatggta tctatagtgt ttttattgtt aggcgctcag tttgaaaatg
    64681 tattgatatt aaatattggt gaaattctca ttcttatagc tacactactt acattatatt
    64741 ctggatggga atatctatat aaaaataaag agctttttat gtcttctaag taaaaaatta
    64801 tgctttaata aataaaaact aatgattaat aaataaaaaa taacaactta aaattttaaa
    64861 aatgtaaaaa agggtatact attatagtat acctcttttc attttaagaa tattttcttt
    64921 tgtgtatata attattaata atggtttata aataaaatgt aatgaataaa aatataatca
    64981 ttagtagcgt ttttaaattg actaggtttg atttatgtta tataattaat atataacaag
    65041 agaacaaatg tttgtagata aatgaaggga tggtatagag atgagtgtag atcaagaaaa
    65101 attaaaagcg ttgaatgaag ctttaggtaa aattgaaaaa gattttggta aaggttcagt
    65161 aatgaaattg ggagaagcaa catctatgtc tatagatgtt atatcaacag gagcgattgg
    65221 tttagacata gctattggta taggaggtct acctagaggg agaatagttg aagtatatgg
    65281 tccagaatct tctggtaaga ctactgttgc gcttagttgt gtagcatcag ctcaaaaaga
    65341 tggaggaata gctgcattta tagatgcaga acatgcactt gacccagtat atgcaaaagc
    65401 tttgggtgtg gatgttgata acctaataat atctcaacca gatacaggtg aacaggcttt
    65461 agagatagca gaggcattga taagaagtgg agcgatagat ataatagtaa tagactcagt
    65521 agcagcatta gttccaaagg ctgaaataga tggagatatg ggtgattctc atgtaggttt
    65581 acaagctaga cttatgtcac aagcacttag aaagttaact ggttcaatta aaaaatcaaa
    65641 ttgtgttgct atatttataa accagttaag agagaaagta ggaataatgt ttggaaaccc
    65701 agaaactact actggaggac gtgcactaaa attctattca tcagttagat tggatgttag
    65761 aaaaatagat acaataaaac aaggtgataa agttataggt agtagaacta gagttaaagt
    65821 tgttaaaaac aaagtagcac caccatttaa gcaagctgaa tttgatataa tgtatggaga
    65881 agggatttca aaaattggag accttttaga tatagctgct gatgtagata tagttaaaaa
    65941 atcaggttca tggtatagtt acaatgatac taaacttgga caaggaagag aaaatgttaa
    66001 aaaattcttg gaagataatt tagatttaac tactgaaata gatgagaaag ttagagcatt
    66061 ttacaattta aatgaagaac atgaagaatc aggtacttca gtatcaaaag aaattgtaga
    66121 agaataagta aaaatacaaa aaaataacta gtaattaaag acatgccacg ctccctatta
    66181 gcttgacaca atgttgaaaa aattataaaa ttaagtgagg ataaaatatc taaatttaat
    66241 tttaattatt aggaatattt tttaatcata gtgatgacat tttgggatta ttaacgaagg
    66301 aggtggatgt catagatagt gtagtaatga ttgtaatagg tgcagctgtt ggtattatag
    66361 ctggatattt tgttagaaaa aatatatctg aagcaaaaat tggacaagct gaaaacttgg
    66421 ccaaagaaat aatagataaa gcacaccacg actcagaaac agtacaaaaa gaaaaattac
    66481 tagaagctaa agaagagatc cataagtgga gaacagaagc tgaaagagaa aacagagaaa
    66541 gaagaacaga agtacaaaag tatgaaaaaa gagtagtaca aaaagaagaa gtcttagata
    66601 gaaaattaca aaatttagaa tctaaagaag taaacctaag tgaaaaatta aaggtagtgg
    66661 aaaagaaaga agaagaagtc gaagtaataa aaactcaaca gttagaaaaa ttagaaagta
    66721 tatcaggaat tacttctgat aaggccaaag aaatcatctt aaccaatgcg gagagagatg
    66781 taagacgtga aatgtctata atgataaaag agatagaaag tcaagctaaa gaagaagctg
    66841 ataagaaatc aagagaaatt ataggatatg caattcaaaa atgtgcagca gatcacgtag
    66901 ccgaaactac tgtaacagta gtaagcttgc ctaatgatga aatgaaaggt agaataatag
    66961 gtagggaagg tagaaatata aggactttag aaaccttaac aggtatagac cttataatag
    67021 atgacactcc agaagccgta attttatcag gatttgatcc aataagaaga gaaattgcaa
    67081 gaattgctct agaaaaatta atagcagatg gaagaataca tccagctaga attgaagaaa
    67141 tggttgaaaa ggcgagaaaa gaagttgaca atattataaa agaatatggt gaacaagctg
    67201 catttgaaac tagtgtacat gggattcatc cggaattaat aagattatta ggtagactta
    67261 actatagaac tagttatgga caaaatgtac ttaaacattc tatagaagtt gctcatatag
    67321 ctggtataat ggctgctgaa ataggagcag atattagatt ggctaagagg gcaggtttat
    67381 tacatgatat aggtaaggct gtagaccatg aaatggaagg tacacatgtt gaaataggta
    67441 tggatttact tagacgttat aaagagtcta aagaagttat acatgccatg agtactcatc
    67501 atggagatta cgaacctcaa acagtagagg ctgtattagt tacagcagca gatgcaatat
    67561 cagctgctag acctggtgcg agaagagaaa ctttagaagc atatataaag agacttgaaa
    67621 aattagaaga aatagctaat tcatatgaag gtgtagaaaa atcatttgct atacaagctg
    67681 gtagagaaat aagaataatg gtaaaaccag aagctataaa tgatgaagaa atacatctat
    67741 tggctagaga tatgactaag aaaatagagg atgaacttga atatcctgga caaataaaag
    67801 taagcataat aagagaaaca agagctatag agtatgcgaa gtaaaagaaa taatatctaa
    67861 ataataagaa aatattttta gaaatttaaa tttgccacaa gatatgataa tatatcttgt
    67921 ggcttttaaa ttgtttaagt ttaattttat ataatgcaaa aataatctaa gtgctaaaag
    67981 tgctacttac aaaagatt
//
LOCUS       DAEPOZ010000003        57373 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.003, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000003 DAEPOZ010000000
VERSION     DAEPOZ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 57373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57373
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.003"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(121..894)
                     /locus_tag="KR440_000128"
     CDS             complement(121..894)
                     /locus_tag="KR440_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HBH0697203.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
     gene            complement(894..1280)
                     /locus_tag="KR440_000129"
     CDS             complement(894..1280)
                     /locus_tag="KR440_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0697204.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            complement(1305..1622)
                     /locus_tag="KR440_000130"
     CDS             complement(1305..1622)
                     /locus_tag="KR440_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HBH0697205.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVKKIFTITGLDKIFK
                     VEG"
     gene            complement(1634..1894)
                     /locus_tag="KR440_000131"
     CDS             complement(1634..1894)
                     /locus_tag="KR440_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBH0697206.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            complement(1894..2190)
                     /locus_tag="KR440_000132"
     CDS             complement(1894..2190)
                     /locus_tag="KR440_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBH0697207.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="KR440_000133"
     CDS             complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="KR440_000133"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBH0697208.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDIAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKIGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKNEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="KR440_000134"
     CDS             complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="KR440_000134"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBH0697209.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            complement(6607..7722)
                     /gene="recF"
                     /locus_tag="KR440_000135"
     CDS             complement(6607..7722)
                     /gene="recF"
                     /locus_tag="KR440_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBH0697210.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="KR440_000136"
     CDS             complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="KR440_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBH0697211.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVKIEGTKIKVL"
     gene            complement(8083..9189)
                     /locus_tag="KR440_000137"
     CDS             complement(8083..9189)
                     /locus_tag="KR440_000137"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBH0697212.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            complement(9431..10753)
                     /gene="dnaA"
                     /locus_tag="KR440_000138"
     CDS             complement(9431..10753)
                     /gene="dnaA"
                     /locus_tag="KR440_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBH0697213.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            11409..11546
                     /gene="rpmH"
                     /locus_tag="KR440_000139"
     CDS             11409..11546
                     /gene="rpmH"
                     /locus_tag="KR440_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBH0697214.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            11624..11968
                     /gene="rnpA"
                     /locus_tag="KR440_000140"
     CDS             11624..11968
                     /gene="rnpA"
                     /locus_tag="KR440_000140"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBH0697215.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            12000..12251
                     /gene="yidD"
                     /locus_tag="KR440_000141"
     CDS             12000..12251
                     /gene="yidD"
                     /locus_tag="KR440_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBH0697216.1"
                     /translation="MNKIIYILKEISRYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRLLKCHPFHPGGYDPLK"
     gene            12283..12990
                     /locus_tag="KR440_000142"
     CDS             12283..12990
                     /locus_tag="KR440_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBH0697217.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            12959..13588
                     /locus_tag="KR440_000143"
     CDS             12959..13588
                     /locus_tag="KR440_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HBH0697218.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            13700..15079
                     /gene="mnmE"
                     /locus_tag="KR440_000144"
     CDS             13700..15079
                     /gene="mnmE"
                     /locus_tag="KR440_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBH0697219.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGERALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNILDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKEIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            15174..17069
                     /gene="mnmG"
                     /locus_tag="KR440_000145"
     CDS             15174..17069
                     /gene="mnmG"
                     /locus_tag="KR440_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBH0697220.1"
                     /translation="MIKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSI
                     ALMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQAD
                     KFKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVY
                     LNSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMS
                     LQEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPR
                     YCPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLEN
                     CKIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINA
                     VRKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLT
                     QKGYDIGLVKKDRYERFLNKKAAVDKEFERLKNERVTPKEVNSLLEEKGATPIKVGIS
                     LYEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLE
                     NKKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQVRR
                     TRGGKGE"
     gene            17062..17781
                     /gene="rsmG"
                     /locus_tag="KR440_000146"
     CDS             17062..17781
                     /gene="rsmG"
                     /locus_tag="KR440_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBH0697221.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVEWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKNEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGRPAKNPIK"
     gene            17956..18741
                     /gene="noc"
                     /locus_tag="KR440_000147"
     CDS             17956..18741
                     /gene="noc"
                     /locus_tag="KR440_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HBH0697222.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            18876..19649
                     /locus_tag="KR440_000148"
     CDS             18876..19649
                     /locus_tag="KR440_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBH0697223.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            19651..20514
                     /locus_tag="KR440_000149"
     CDS             19651..20514
                     /locus_tag="KR440_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBH0697224.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            20547..21689
                     /locus_tag="KR440_000150"
     CDS             20547..21689
                     /locus_tag="KR440_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBH0697225.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKSNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVSDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(21823..22398)
                     /locus_tag="KR440_000151"
     CDS             complement(21823..22398)
                     /locus_tag="KR440_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HBH0697226.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDTSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKTQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            22658..23950
                     /locus_tag="KR440_000152"
     CDS             22658..23950
                     /locus_tag="KR440_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBH0697227.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSENKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            24055..24657
                     /gene="yedF"
                     /locus_tag="KR440_000153"
     CDS             24055..24657
                     /gene="yedF"
                     /locus_tag="KR440_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBH0697228.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            24730..24993
                     /locus_tag="KR440_000154"
     CDS             24730..24993
                     /locus_tag="KR440_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HBH0697229.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            25008..25193
                     /locus_tag="KR440_000155"
     CDS             25008..25193
                     /locus_tag="KR440_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBH0697230.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            25296..26492
                     /locus_tag="KR440_000156"
     CDS             25296..26492
                     /locus_tag="KR440_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBH0697231.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLEKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            26706..26984
                     /locus_tag="KR440_000157"
     CDS             26706..26984
                     /locus_tag="KR440_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBH0697232.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            27021..27455
                     /gene="ssb"
                     /locus_tag="KR440_000158"
     CDS             27021..27455
                     /gene="ssb"
                     /locus_tag="KR440_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBH0697233.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            27474..27701
                     /locus_tag="KR440_000159"
     CDS             27474..27701
                     /locus_tag="KR440_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBH0697234.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            27844..28164
                     /locus_tag="KR440_000160"
     CDS             27844..28164
                     /locus_tag="KR440_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBH0697235.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            28172..29128
                     /locus_tag="KR440_000161"
     CDS             28172..29128
                     /locus_tag="KR440_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HBH0697236.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            29139..31133
                     /locus_tag="KR440_000162"
     CDS             29139..31133
                     /locus_tag="KR440_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBH0697237.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            31134..31583
                     /locus_tag="KR440_000163"
     CDS             31134..31583
                     /locus_tag="KR440_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBH0697238.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            31602..32930
                     /gene="dnaB"
                     /locus_tag="KR440_000164"
     CDS             31602..32930
                     /gene="dnaB"
                     /locus_tag="KR440_000164"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBH0697239.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            33135..33443
                     /locus_tag="KR440_000165"
     CDS             33135..33443
                     /locus_tag="KR440_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697240.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            33525..34814
                     /locus_tag="KR440_000166"
     CDS             33525..34814
                     /locus_tag="KR440_000166"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBH0697241.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNKIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            35140..35215
                     /locus_tag="KR440_000167"
     tRNA            35140..35215
                     /locus_tag="KR440_000167"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35173..35175,aa:Lys,seq:ttt)
     gene            35222..35296
                     /locus_tag="KR440_000168"
     tRNA            35222..35296
                     /locus_tag="KR440_000168"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35255..35257,aa:Glu,seq:ttc)
     gene            35301..35376
                     /locus_tag="KR440_000169"
     tRNA            35301..35376
                     /locus_tag="KR440_000169"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35334..35336,aa:Val,seq:tac)
     gene            35410..35484
                     /locus_tag="KR440_000170"
     tRNA            35410..35484
                     /locus_tag="KR440_000170"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35442..35444,aa:Thr,seq:tgt)
     gene            complement(35867..36859)
                     /locus_tag="KR440_000171"
     CDS             complement(35867..36859)
                     /locus_tag="KR440_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0697242.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKEKLLSESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(36868..37926)
                     /locus_tag="KR440_000172"
     CDS             complement(36868..37926)
                     /locus_tag="KR440_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBH0697243.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNGEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            38051..39514
                     /locus_tag="KR440_000173"
     CDS             38051..39514
                     /locus_tag="KR440_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HBH0697244.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            39616..40413
                     /locus_tag="KR440_000174"
     CDS             39616..40413
                     /locus_tag="KR440_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0697245.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            40426..42528
                     /locus_tag="KR440_000175"
     CDS             40426..42528
                     /locus_tag="KR440_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HBH0697246.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVIMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTITSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEKEI"
     gene            42596..43075
                     /gene="rlmH"
                     /locus_tag="KR440_000176"
     CDS             42596..43075
                     /gene="rlmH"
                     /locus_tag="KR440_000176"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBH0697247.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIVLAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSKMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            43224..43535
                     /locus_tag="KR440_000177"
     CDS             43224..43535
                     /locus_tag="KR440_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0697248.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            43583..43900
                     /locus_tag="KR440_000178"
     CDS             43583..43900
                     /locus_tag="KR440_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBH0697249.1"
                     /translation="MDEKIIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SKEVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            44113..44814
                     /locus_tag="KR440_000179"
     CDS             44113..44814
                     /locus_tag="KR440_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0697250.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKKTIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            45016..46317
                     /locus_tag="KR440_000180"
     CDS             45016..46317
                     /locus_tag="KR440_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0697251.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKATLDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            46454..47548
                     /locus_tag="KR440_000181"
     CDS             46454..47548
                     /locus_tag="KR440_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBH0697252.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMVSKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNEYQVVFNVVAKEKTSEVEKIIMVNEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNKNIFKRGDVVIGNDDFGKYKGELQLILQDCTDERK
                     NKVGRIKEDEIILLDFIEPWSKFKIILEGE"
     gene            47550..48683
                     /locus_tag="KR440_000182"
     CDS             47550..48683
                     /locus_tag="KR440_000182"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HBH0697253.1"
                     /translation="MKIDILIKCGKTVDKSIIDIAILDDKIIEVKNHIDDSKYQAKQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGEFY
                     EDTKKYKTNVYALINIAKQGITSQDELSNMMNIDEYELKNAVRKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEAKERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGNSIEENE"
     gene            48701..49810
                     /locus_tag="KR440_000183"
     CDS             48701..49810
                     /locus_tag="KR440_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HBH0697254.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFL
                     IIEELVNKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEINFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDNIYNRIKNIVES"
     gene            49875..50615
                     /locus_tag="KR440_000184"
     CDS             49875..50615
                     /locus_tag="KR440_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HBH0697255.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGHVLVGVLSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGDTKVEDIGVLYKIMKELLG"
     gene            50620..51612
                     /locus_tag="KR440_000185"
     CDS             50620..51612
                     /locus_tag="KR440_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBH0697256.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYIITGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEEMDIVHICGIAMQLTDSVKKIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIDIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(51799..52680)
                     /locus_tag="KR440_000186"
     CDS             complement(51799..52680)
                     /locus_tag="KR440_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0697257.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            52953..54341
                     /locus_tag="KR440_000187"
     CDS             52953..54341
                     /locus_tag="KR440_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697258.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPNNPVEIGKWWRPDQYADGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFMPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKAGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            54369..55322
                     /locus_tag="KR440_000188"
     CDS             54369..55322
                     /locus_tag="KR440_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBH0697259.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLESGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIEPLTKEKQAGVSLG"
     gene            55382..55606
                     /locus_tag="KR440_000189"
     CDS             55382..55606
                     /locus_tag="KR440_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697260.1"
                     /translation="MNEKPSFKQIEIILAVIFLVLTYYKIGLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            55599..56111
                     /locus_tag="KR440_000190"
     CDS             55599..56111
                     /locus_tag="KR440_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBH0697261.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDKKKGN"
     gene            56143..56997
                     /locus_tag="KR440_000191"
     CDS             56143..56997
                     /locus_tag="KR440_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HBH0697262.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
BASE COUNT        21693 a         7031 c         9542 g        19107 t
ORIGIN      
        1 atataaatta acctactgtt taattttcaa tgttcgtgtt tatttcttgt tccttttttc
       61 aaggacaagt aatactatac catctttaaa acagtaggtc aatacttttt ttatattttt
      121 ttataaattt ttttcatatt cttttttcag cttctctact acctttttct ctagtctaga
      181 aacagtcatc tgtgatatcc ctagttcttt agctatacta gattgtgttt tatcaaagaa
      241 aaatctatct ttaaaaattt taacttctaa ttcatttaaa gtttcaataa acttattaat
      301 aaaatcttca tactctatac ttccaaagtt accttcttct tttccaatct tatcaataag
      361 agttatatct ttatcctctg aatcatcatt acttgatgaa tccaaagaca ttggttgata
      421 tccataagaa gcctccatag cctctaaaac atcttcttca ctaacgccta tataatcagc
      481 tatatctttt acctttggat gcttcttatt ttcttgttct aatttaatct tcgcatcact
      541 tatctttttg cttaattcct gtattcttct tgggactcta agtgtccata ccttatctct
      601 aaaatacttt tttatttctc ctactatagt tggtgtcgca aagctggaga actcaaatcc
      661 tttctcaaca tcatatctat caatagcata aattaatgcc aatgaagcaa cttgatatat
      721 atcttcaaaa tcaacacctt tgtttatata cttcttagct aacaatcttg ctaaatacag
      781 atgtctttca ataagaatat ttctaatgtc tacattttta tttttattat ataaattaaa
      841 tagctctttt gtatccatat ttaggtaatg tgtagcatta gctacatttt tcattaaata
      901 tcaactccta aatatttagt catttttatt tttgtccctt gattatgttc tgattcaata
      961 tctacatcat ccattaaagt tttaattata aacaatccaa gaccactctc ttttggattc
     1021 tctaaatctg gtgtacttat tgagctaaca tcatatcctt ttccattatc ttgaatttct
     1081 atattaattg cattatctaa aatattaaat actatattaa acacatcatc tttactgtgt
     1141 tttattgcat ttgtacatgc ttcagatact gctaccttta tatcttctat atcatctatt
     1201 ggaaaaccca tcttattggc aattccagat gctgttaatc ttattatact aacataatct
     1261 ggatttgtag taatttccat ctttatagtc tcacaagcca aagtttatcc ctccacttta
     1321 aatattttat ctagacctgt tatagtaaaa atctttttta cgttactctt aggatttact
     1381 atgtatatct ctttttcatt tatttttaat ttttttaata cacctatcat agctcctaag
     1441 cctgttgagt ctatataatc taaatcttta agattaatct ttacatctaa catatttttt
     1501 tctattaaag catgtaaatg ttcctttagt ttatctgctg tagaaacatc aagttctcca
     1561 tcaagacaaa cattccaaaa tttattttgt gaatctaaat ttgaatcaat attcatagac
     1621 atgtaaaaac ctcctatttc cttttaataa ttttaaatat atttcctata ctcaatattt
     1681 tattaattac ataaactata tcatctattc cctttgatat tgatgatgca ttttcaacaa
     1741 tatcttgtat atgtctttca ttataatcta caatcttata tgccttttca acaaccttat
     1801 ttatgctgtt tagagtttta gctaaataaa ttgctactat aagagcagag cttcctacta
     1861 acactgctcc aatttgccac ccccatgcat tcattagtta acatctccct catcatcaaa
     1921 gcttttactt attactatat cttcttctga aatctttata tcttcttcat tattatcatc
     1981 gtccactaaa tttattattc tctctttaac atcattaaat gtttcatgta gaacttcttt
     2041 tggattttct ttgacatcat taaacttctc ttttgtttct tttcttagtt cagaaccttt
     2101 tttaggtgca aaaaacatac caaatataaa ccctaaaata gctcctaata ataaaaatcc
     2161 acctttttta caacttcttt tattgcacat aacaattact cctttcaatt ttaaattttt
     2221 atctaactac attatacaaa aagaatagga tatttatcct attcttcttc aatatttatt
     2281 ttggcaatcg aaactacatt gacttcatca ttcgtcttca ttaatttaac accacttgta
     2341 actcttccaa acagtgatat ttcattgact cttattctaa taagaactcc atcagaattt
     2401 ataatcatta tctcatcatc ttcgttgacc atatctgcac ctacaattgt accagttttt
     2461 tcagatatat tataagtttt aattccttta ccagctctta tttgacttct atactcttct
     2521 atatttgttc tctttccaaa accattctta cttacaacta acacatctgt acctttactg
     2581 caaaggttca tagacacaac aaaatcttct ttacttaaag ttataccttt tacacccatt
     2641 gctgttctac ccatatatct tatatcattt tcatcaaatc ttatagacat accttcttta
     2701 gttactaaaa gtacttcttg ttttccatct gtaagttcta ctccaataag ctcatcatca
     2761 tctcttaaac ctatagcaat aagaccagat ttatttatat ttttgaactc ttctctctta
     2821 gtctttttaa caataccttt tttcgttgca agtaataaat attcattttc atcattacca
     2881 tcaataggta ttagagtagc aattttctca tctgctgaca attgaagtaa attcactata
     2941 gcagtacctt tagcctgtct cttaccttct ggtatttcat atgcatttaa tttaaatact
     3001 ctacctttat ttgtaaagaa taatagtcta ctatgagtag ttgtagttac caagtgcctt
     3061 acaaaatctt cttctctagt tgtaagtgct gaaatacctc ttccacccct tttttgactc
     3121 ttataagtat cagatggaag cctttttata tatccaaagt gagtaagagt tattgctatt
     3181 tcttcatcac ttataagatc tctcatatct atttcgcctt cagcatgtct tatttctgtt
     3241 cttctctcat cagagtaatt ttcttttatt attgtaattt cattctttat tacatttaaa
     3301 agtaatcttt catcagctaa aatttctttt aatctattta tcttcttgat taaatcttca
     3361 tattcagctt ctatcttatc tctttctaaa cctgtaagtc tttgaagtct catatctaat
     3421 atagcttgtg cttggatttc agttaatttg aatttttcta ttaaacctat cttagcttct
     3481 tgcccagtct ttgaagctct tatcaagctt ataacagcat ctatattatc taaagcaatc
     3541 tttaatcctt ctaaaatatg tgctcttgct tcagctttat ttagttcaaa tttagttctt
     3601 cttgtaacaa catcttcttg atgtttaata taatgatata atatttgttt aagatttaaa
     3661 actcttggct gaccatctac aagtgcaagc attattatac taaaagtatc ttccatttga
     3721 gaatgtttat acaaattatt taatactata ttagcattag catccctctt taattctata
     3781 acaattctca taccatttct attactttcg tctcttaggt ctgatatacc ttctattctc
     3841 ttttctttaa ctaactctgc tattctttca accagtttag ccttatttac ttgataaggt
     3901 atttctgtaa ctattatttg ctgttttcct tttggtagtt cttctataaa agctctagac
     3961 ctaactttaa cttttcctct tccagttctg tatgcttctg ctatactttc ttttcccatt
     4021 ataattgcag ctgtagggaa atctggtcct tgaacaaatt ttattaaatc atctacacta
     4081 cactctggat tatctatcaa atatacagtt gcatcaatta cttctgctaa attatgtgga
     4141 ggtattgaag ttgccatacc aacagctata ccatttgagc catttactaa taaattagga
     4201 tatctagctg gcaatactga aggctctttt aacgactcat caaagtttgg tttaaagtct
     4261 acagtttcct tttcaatatc tctcagtagt tctaatgata atttactcat tttagcttct
     4321 gtataacgca tagcagctgg tgaatcccca tcaacagaac caaagttacc atgaccatct
     4381 actaaaagtg ctctagttga aaaatcttgt gccattctta ccatagcata ataaacagca
     4441 atatctccat gaggatggta cttacctaaa acgtccccaa caatacgagc tgacttcctg
     4501 tatggtttat ctggagttaa atttaactca ctcattgaat ataatattct tctatgaact
     4561 ggctttaaac catctctaac atcaggaaga gcacgtccag ctataacact cattgaataa
     4621 tcaatatacg attttttcat ttcttccgct atttcaatag ggagtatttt gttattttct
     4681 tccatgtata ctaatcccct ctctctatat atctaaattt ctaacaaatc ttgcattttc
     4741 ttctataaat tcttttcttg gagcaacctt atcacccata agcattgaaa atacttcatc
     4801 tgcaattgca gcatcttcta cagtaacttg taataaagtt cttgtctcag gattcatagt
     4861 tgtttcccat aattgctctg cattcatctc tccaagacct ttgtaccttt gaagttcaac
     4921 accatttctt ccaatttcat ctaataagat atttaactct ttatctgagt aaacataatg
     4981 ttccttcttt tgctttgtca ctttatataa aggtggctgt gctgcataaa catacccctc
     5041 atctataaga ggtctcatat atctaaagaa gaatgttaat aataaagttc ttatatgggc
     5101 tccatctaca tcagcatcgg tcataattat aattttatga tacctagcct tatctatatc
     5161 aaaatcttct ccaataccac aaccataagc tgttatcata ttttttattt catctgaaga
     5221 tagtatccta tctagtcttg atttctcaac attaagtatt ttccctctta atggaagtat
     5281 agcttgacta tttctatctc taccttgttt agctgaacct cccgctgaat ccccttcgac
     5341 taagaatatt tcacttttag atggatcttt ttctgcacaa tctgcaagtt ttccaggtaa
     5401 agatgtactt tctaatacac tttttctcct tgttaattct cttgcttttt ttgcagcttc
     5461 tctagctctt tgtgctctta aagctttatc aactatgatt cttgctgttg atgggttttc
     5521 ttctaggaaa gaccccagtt catcaactgt tacactatca actatacctc tcataaagct
     5581 attacctagc tttgttttag tttgaccttc aaattgaggt tctggtaatt taactgatac
     5641 tactgctgta agaccttctc ttatgtcttc accaagtagg ttaacatcat tttcctttaa
     5701 aaatttattt ctcttagcat aatcatttac agttttagta agagctgctt taaatccact
     5761 taaatgagaa ccaccttcgt gtgtatttat attgtttgca aaagaatata tgttctctgt
     5821 ataaccatct gtatattgca tagctagttc aacaaaacaa tcatcaacct ttttatctat
     5881 ataaactatg tcatcatgta taccagttct agatttattt aaatacttaa tatattctac
     5941 caaaccacct gtatagtgaa attcttttct tttttcttgt ccttctcttt tatcttcaaa
     6001 tactattttt attcctttat tcaagaaaga aagttctcta agcctatact ccaatgtttc
     6061 atatttaaac tctatttcat caaatattgt ctcatctggc ataaattgaa tcattgttcc
     6121 tttatcttga gattctccaa ctacttcaag cttagatgtt ggtaatcctt tctcatatgt
     6181 ctgcttatat atctttccgt ttaaataaac ctctgctacc atccattttg acaatgcatt
     6241 aactacagaa acaccaactc catgaagacc tccagatact ttatatcctc cgcctccaaa
     6301 ctttcctcct gcatgaagat tagttaatac tgtttctaaa gttgatttac cagttttagg
     6361 atgtatttca acaggtatac ctcttccgtt atcttttact aaaacactgc catcttcatt
     6421 aatagataca tatatgtctg aacaatatcc ttgtaaagcc tcatctatac tattatctac
     6481 aacttcataa actaagtgat gtaaacctct agggcttgta ctaccaatat acatacctgg
     6541 tctttttcta acggcttcta atccctctaa tacttgtatc tgacttgcac cgtattcttg
     6601 tttcatttaa tttcctccat tctctatgct aataacatcg ccattttcaa tattaaatat
     6661 tgtaacattt ttttcatcaa atattttttt atgtgaaact tcagcacttg ttataaacat
     6721 ttgaacattg cttaaattat ttactaaaag tttttgtctt gcttcatcta actcactaaa
     6781 aacatcatct aaaattaata ctggatattc ttcaacttca ttttttatta attctatctc
     6841 tgataacttt aatgatattg aagcagttct ttgttgacct tgtgaaccaa aaagtcttgc
     6901 atctaaatca tttatgaata tatttaagtc atctttgtgt ataccatatc tggtagtctt
     6961 agattctata tcattaggtc tattcgatga caacttggct aaaaatttat tatatactgt
     7021 atctatagta tcttcatcag ttatatttat ttgattttta tatcgaattg ataatctttc
     7081 aactccatta gtcaaattca tatgcatatt ttcagatata ttagctatct tttttataaa
     7141 gtctctacgc aaaatataaa tataacttcc atactttgct aacgtatcat catatacatc
     7201 taataaagct tcatcaacat gtatattttt aagcacccta ctcctttgag aaagtgtttt
     7261 attataattg gtaagatatt tataatattt aggtataatc tgactaatct ctttgtctat
     7321 aaatgccctt ctttctttag gaccctcttt aaccaacctt aaatcttcag gagaaaatat
     7381 aacaacattt aagttgccaa gtagttcttg tatcttttgt aaaggaactt tatttattct
     7441 tatccccttt ttatcttttc caataattag ctctattaga ctatatttat tatattttga
     7501 aaaacttcct ccaatgtata agttttcact attaaatcta accatttctt tatctttgtt
     7561 agtcctaaat gattttccaa aagaaagcat ataaatagat tctactatat ttgtctttcc
     7621 ttgaccattt ttaccaacaa gtagatttac tttcccatta aattccagat gcaacttttt
     7681 ataatttcta aaattaacaa gttgcaaact tttaagtttc acaaaaagtt ctcctttttt
     7741 tacaatactt ttattttagt tccttctatt ttaactatat ctcctgattt tatctttttt
     7801 cctcttctta attctatttc atcatttact gtcactaaac cttcttgaat taaaaatttt
     7861 gcatgacctc ctgttgatgc aatctctgct aatttaagaa attgatccaa ctttatatat
     7921 tctgattcta tagttatttc agtcataata gttcctcctt gtcactattt taaacaaatt
     7981 cctattgata cactaaaagt atattatacc ataaatatgc ttataaaagg agttgtctaa
     8041 gattagaaaa attttctaca aaagacaact ccttcatgtc tgctatatat ttgatgatat
     8101 tcttactgga agcagtaagt aaatataatt aacatcatct gttggtttaa ttatacaagg
     8161 atttacattt gtagtaaatt ctataaatat ttcctcatta tctatatttt tcaaaccttc
     8221 gataaaatat ctagaattga aagctatatc taggtaatct ccatctaaat ctatttcaac
     8281 ttcttcatac acatttcctt tttctgtgtt agaagtaatt gccattactt tatctcttat
     8341 agataacttt ataagattat ttttttctga ttgagacaat aaagacgctc tctctatact
     8401 atttaaaagt tccttagtgt ttaatttaac tctgctgtta tgttctcttg gcaataattt
     8461 tttataatca ataaaatctc cttcaagcaa tcttgtaata attttcgtat cattaattat
     8521 aaaaatagca tttttttcgt taaacccaac tttaacatta tcctctccag aaagcaaact
     8581 atttacatct ataagtgtct ttccaggaat tatgactttt atattttcag ttaaactatc
     8641 aactgaacaa ctttttacag ctaatctata tccatcaatt gctactaaat ttaagtttct
     8701 atctacaatt tctaaaagtt ctcccattaa aacaggtttt gtttggtcct gtgagatagc
     8761 aaatacagtt tgtttaatca tattttttag aatttcttga ggtatgctat ataaatcttc
     8821 ttcatttaat tctggtaatt taggaaattc ttttgctgca taacctttaa ttttaaatct
     8881 tgaatttaca caatttatgt aaatattatt ttctgaatca gtttctatct caacaaagga
     8941 atctggtaat tttctaatta tatctccaaa tagccttgca tctacaacaa catcaccttt
     9001 ttctatgatt tctgcttgta cataagtttc tattcctatt tcagcatcat atccagtaag
     9061 ttttaattga ccttcttctg tagatataag tatacctttt aataattcta ttgttgtttt
     9121 tccattaata gctttttgag caattccaat tctatttgct aggatttttt gattacaaat
     9181 tattttcaat gtggataacc tcctctggct ttttatatat aaaaataagt taaaatcata
     9241 gtagtaatag tagtaggtgt tgttgataat gttaataagt atgtataaca ttataaatac
     9301 aaagatatta acatgtgtat aagatgttaa tatctttgtt attttagact tgataatttg
     9361 ttaataattt ttgttcaaaa aaagtattaa caggttattc aagagatata aacatacaat
     9421 tgtgtataaa ttatcccttc aaatctgata taattttgtc tattttagtc ttaatttctt
     9481 cactttcttc tatatcctta gatactttgt catgagcatg aattactgtt gtatggtctc
     9541 tacccccaaa ctcttcacct attttaggaa gggataaatc agtaagctcg cgcgttaaat
     9601 acatagctat ctgtcttgga taagctattg aacgtgttct ttttttagaa ttaaaatctt
     9661 ccatctttaa attgaataca gaagatactt tttcttttat tctaagaaca tttatttctt
     9721 catttttaga agttgttata atatctttta aagcttctgt agcaagatca aaagatatta
     9781 ccctattgct aagagaagaa taagcaacca ctctagttaa tgcaccttct agttccctta
     9841 tgtttgactt aatatttttt gctatatatg acataacctc atttggcaca tctatacgtt
     9901 ctagttgagc cttttttcta agtatagcaa ttcttgtctc aaagtcaggt gcttgaatat
     9961 cagttataag tcccatttca aatctcgatc ttagcctatc ttctagtgta ggtatgtctt
    10021 ttggcggtct atcactagat ataattattt gtttgtttgc ttcatgaaga gtattgaatg
    10081 tatggaaaaa ctcttcttga gttctttctt ttcctgctat aaattgaata tcgtcgatta
    10141 gtaatacatc tacgtttcta tatttattcc taaattcttc gtttttatcg tcttttatag
    10201 agtttataag ttcattagta aatttttctg aagaaacgta aactactttt gagtcttttt
    10261 tttgactaac aatatgatga cctatagcat gcattaggtg agtcttacct aatccaactc
    10321 ctccatacaa aaaaagtggg ttataagctt tagctggaga ttctgcaact gcaacgcatg
    10381 cagcatgtgc aaatctattg ctattaccaa taacgaatgt atcaaatgtg tattttggat
    10441 ttagattggg gtaaagaact ctataattta aatctgtgct atcagaattt aaatcagcaa
    10501 cctctttttc gctcaataca aatttaatat tatatttttt taatgacagt tgactaatag
    10561 catcttcaat taaatgtaaa tatctattct ctaagatatc tttattaaaa tcactaggag
    10621 ccagtaagat taaatcatta agatgtattt ttaatggtac gatgttttta aaaaaggtat
    10681 taaaacttac tgaagttaag tcacctttta ttaattgtag ggttttgtcc cataaagaaa
    10741 ctatatccat aatataaacc tccgaacaga ataattaata ttttccacaa agttattaac
    10801 agaaaagcaa aaatatatat aaattttaca gaataactaa atttatggat aatggtgttg
    10861 ataaattaat aatagtataa attattaaaa aaatatgaaa agatataaac aatatgtaga
    10921 taaagttata aataacataa acatacaata aatggtgatt ataactagag tattgtcagt
    10981 aatcttaaaa gttatacaca aaagtatcca caagctgtga ataatttcta ataagttgat
    11041 aacatttatg cacattatca acaagtatat ttataatatc aaaatatatg tcaaacttca
    11101 atgatttgta actaatttat ccacaatatt aactaattgt tgataaaaat ataaacatgt
    11161 taaaattact ttatttgtgt atataattga aaaaaataat ttagaataaa attaaataga
    11221 tttttgctag tattttcaat aaaaaaaata cataatatat atatggtaga ttttgtgata
    11281 taagcgtccc aaaaactcgt tgacaacagt agtaaaagta tttataatta tatgtgttat
    11341 gtttactttg agggtaaagt tattattttt ataaacggaa aataaaatct gaaaggcggt
    11401 gcaaaataat gagtaaaaga acttatcaac caaaaaagag acaaagaagt aaagaacatg
    11461 gtttcagaaa aagaatgaag acgtctaacg gaagaaacgt gttaaagaga agaagagcta
    11521 aaggaagaaa tagattaact cattaattac aaaagggccg ctgcaaaggt ggcctttttg
    11581 tgtaaataat tacaattcat agctgtgttt atggaggtta actatggact ttaataggac
    11641 taaagggttg aaaaaagact ctgattttag aaaagtatat aaacacggca aatcttttgc
    11701 aaacaaatat ttagtaatat atatacttaa aaataaatca gattatagta gagtaggtat
    11761 ttctgtttca aaaaaagtag gaaaagctat tactaggaat agagtaagaa gattaataaa
    11821 agaagcctac agattaaata ttgatgaaaa aattaagcct ggatatgata tagtatttat
    11881 agcaagggta tctagtaagg atgctacttt taaagacata gacaagtcta taaaaaattt
    11941 agttaaaaga acggatattt ctatttaatt gtatgctata aatatggagg ttaatgactt
    12001 tgaataaaat aatttatata ttaaaggaaa taagtaggta tttatcgaat ttatgtattt
    12061 atttagtgag attttatcaa aagtatatat ctcccttaaa gggacctacc tgtagatttt
    12121 atccaacttg ctcacaatat tcaatagagg cgtttaaaaa atatggattt gtaaaaggta
    12181 tgtatttaac gataagaaga cttttaaaat gtcatccttt tcatccagga ggttacgacc
    12241 ctctaaaata aaagagagta actattagga ggtataaaaa aattgaatat aataggaaat
    12301 ttcttaggat tgattcttaa ggtaatattt gaatttgtta atcattatgg tatttctata
    12361 atacttttta cgatacttgt aaagattatt ttattaccat taacaattaa gcagactaag
    12421 tcaacaaagg caatgcaaga tatacaacct cgtataaaag agatacaaga aaaatataaa
    12481 aataagccag aaaaacaaaa tgaagaaatt gttaagttat atggagaagc taaaattaat
    12541 ccactatctg gttgtttacc tttattaata cagtttccta tattaatagg attatttagc
    12601 gttttgagag aaccagttgc tcatggcgtt tttgcaaaca aagctgcatt tttagcagct
    12661 gacaatgggt ttttatggat aaaaagctta acatcaccag attatgtatt agcagtattt
    12721 tcaggagcaa gtgcatatgt catgcaaaaa gtaatgacac ccaaagatca gttacaaggc
    12781 tctatgaaag taatgacata tgtaatggca ggtatgtctt tctattgggg atttatattc
    12841 ccagcaggac ttacattgta ctggacagta agtaacttat tctcaatagc tcaatattac
    12901 ttaataatga atcctttaaa agctaaactg gctgctaact ctaaggagga aaaagttaat
    12961 gagaaaaagc ctaaaattaa taaaaagtaa gagtaaagag gaagctataa gtaaagctat
    13021 agcagagttg aatttaaaag cagaggatat agaagtagaa atacttgaaa atccaagtaa
    13081 agggttttta ggttttatag gtgctaaaga tggtacttat gaaatttttg ttatcgagag
    13141 ggaagtagat gtagctaaaa attttataga ggtaatgttg aaaaatgcaa atgttgatgc
    13201 caaagtcaat gtatctcaaa aagataattt aatcatggtt gatattgaag gcaaagaagc
    13261 tgcaagttta attggtagaa gaggggaaac tttagattct attcaatttt tgactggatt
    13321 agctcttaat aaaataaata aagattctca tacaagagta cttgtagata tagaaaatta
    13381 tagatcaaaa cgagaagaat ctttaataag atatgctaat aaagttgcta gagaagtagc
    13441 gaaaacaaga aaaactaaaa aattagatta tatgaatcca tatgagagaa gaataataca
    13501 ttcagctctt caaaatgaca aatatgtaat tacatatagt gaaggaaccg atccatatag
    13561 aagattagta attgagtgta aaagataaag tcaacctcct aaacattagg gggttttatt
    13621 ttttaatatt attaaatcat taatgattgg ttaaatgtaa acctatatgt aaaaaattat
    13681 gaattaagag gtgataaatt tgtttataga tgatacaatt gcagcaatag ctacagcacc
    13741 tggagaaggt ggtataggta tacttagaat aagtggagaa agagctttga aggttgcaga
    13801 agaaatcttt aaatctatgt cgggaaagag tattgaagaa tacaataaga gaacattaat
    13861 atatggaaat atattggata atgaaaatat aattgatgaa gttttattgg catatatgaa
    13921 aggaccaaac tcctatacag gagaagatgt tatagaaatt aattgtcatg gtggatttat
    13981 ttcggtaaag aagatattag aattaatact atcaaaagat gttagattag cagaggctgg
    14041 tgagtttact aaaagagcat tcttaaatgg aagaatagat ttatctcaag cagaggctgt
    14101 aattgatgtt ataaaagcca aaactgatat agctcatgaa gttgctcaaa atcaattaga
    14161 gggttcttta tcaaagaaga taagagaatt gagagataaa gtcactgaga tattagctca
    14221 tgtagaagtt gctatagatt atcctgagga agatattgag catataacat atcaaactct
    14281 taaagaaaaa actgatgaac ttaaaaaaga gattaaaaaa ttatatgata cagctgaaag
    14341 tggaaaaata cttagagaag gcttaaagac ggtaattgtt gggaaaccaa atgtaggtaa
    14401 atcttcacta cttaattcta ttttaggaga gaatagagca atagttactg atataccagg
    14461 aactactaga gatgttatag aagagtttgt aaacataaaa ggtatacctt taaaaattgt
    14521 tgacacagca ggtataaggg atactgatga tatcgttgaa aaaataggtg ttgaaaagtc
    14581 taaagaatca tttactagtg cagatttgat agtaatggtt ttagatgcat ctagaaagtt
    14641 gtcagaggaa gatatagaga tattagaaaa acttaaggat aaacaaacaa tagtgctgtt
    14701 aaataagaat gatttgaaac aagagattga agaagaaaaa atacttaaat atgtagaaaa
    14761 taatagtata ataaaaatat cagctttaca acaagaagga atagaagaat tgcaagataa
    14821 aatagagtct atggtatata aaggtagtat caaaaataat tctagtttag tagtaacaaa
    14881 ttcaagacat aaagatgcac ttagtaaagc atataaatct gcaacagatg ctttaattgc
    14941 tttggaacaa agtatgccat ttgactttgt agaagtcgat ttaaaaaata tatgggacta
    15001 tcttggatat attaatggtg atacagtaac agaggattta ctagataata tattccataa
    15061 tttctgtatt ggtaagtaat gtacaaaagt aaatttgttt ttattataga gatttttaaa
    15121 aaatttgaag tgtttctcat gaaacattaa ttttgaaaag aaaggaatta taaatgataa
    15181 agtttgaagc aggaaaatat gatgttatag tagtcggagc aggacatgct ggatgtgaag
    15241 ctgctttggc tactgcaaga atgggatata aaactttgat aataacaatg tctttggatt
    15301 ccatagcatt aatgccttgt aatccatcaa ttggagggac tggtaaaggt caattagtta
    15361 aagaaataga tgccttaggt ggtcagatgg gattaaatat agacaagaca tatatacaaa
    15421 gtagaatgtt aaatactgcc aaaggtccag cggtacactc tttaagagca caagcagata
    15481 aatttaaata tcatgaagaa atgaaaaaga cattagaaga tgaaccaaat cttgatatag
    15541 ctatggatga agttgtagaa atacttcatg aaggaaatgt tgtaatagga gttggaacta
    15601 aactaggatg ttcatttaaa tctaaagctg taatattggc cactggagtt tatctgaact
    15661 caaagatata tatgggtgaa gttgcatttt atgaaggtcc taatgcattg gggtatgcaa
    15721 aatatcttac agatagctta gttgagcttg gtcttagaat gagaagattt aagacgggaa
    15781 ctccagcaag agttcataga gatagtatag acttctctgt aatgtcttta caagagggcg
    15841 atgaaaaagt aactccattc tcatttatga atgaaaatat agaaaaaaaa caagaacctt
    15901 gttatttaac aagaactaca gaagaaactc aaaaagttat actggataat ttaaaaaggt
    15961 ctgctatgta tagtggagta atagaaagta ctggtccaag atactgtcct tctatagaag
    16021 ataaagtagt aagatttagt gataaaacct cacatcaatt atttatagaa ccagaagggt
    16081 taaatactaa agaaatgtat atacaaggaa tttcaactag tttacctttt gaagttcaat
    16141 tagatatgta caaaactata aagggacttg aaaattgtaa gataatgaga cctgcatatg
    16201 ctattgaata tgattgtgtt gacccaactc aactaaaaat aagtttggaa ataaagggtg
    16261 tcgagaactt atttagtgca gggcaattta atggaacttc tggatatgag gaagcagcag
    16321 ctcaagggct tatggctggt ataaatgcag ttagaaagat agaaggaaaa gagccgtttg
    16381 tattagaccg ttcagaagct tatataggtg tcttattaga tgaccttgtt actaaaggaa
    16441 ctaacgagcc ttacagaatg atgacatcaa gagcagaata tcgactatat ttaagacaag
    16501 ataatgcaga tatgagatta actcaaaaag gttatgatat aggattagtt aaaaaagata
    16561 gatatgaaag gttcctaaat aaaaaggctg ctgtagataa agaatttgaa agattaaaaa
    16621 atgaaagagt tacaccaaaa gaagtaaata gtttgttaga agaaaaagga gcaacaccaa
    16681 taaaggttgg aatatctcta tatgaatttt taaagagacc agaggttact tacgaattgc
    16741 ttgaagaatt agggaagggc gctggagaag atgtttcaag agaagttaaa gaacagtgtg
    16801 taataataac taaatatgaa ggttatatag agaaacaatt gaaacaaata gaccaattta
    16861 aaaaacttga gaataaaaag ctggatgaga aaataaatta ttcatctata gaaggtctta
    16921 gattagaggc tagacagaaa ttagatgata taaaacctat ttcaataggg caagcatctc
    16981 gtatatcggg tgtttctcca gctgatatat cagttttact tatataccta gaacaagtca
    17041 gaagaactag aggtggcaaa ggtgaataat atagaacttc tgaaaaacgg gattgaaggt
    17101 tttaatatag atacgaatga cagtatgtta gaaaaattta aaatctatag agagatatta
    17161 gttgaatgga ataaaaagat gaatcttact ggaatagaag atgaaaaaga agtgtatata
    17221 aaacattttt tagactctat ttcagctgta aagaatggat atataaaaaa tgggatgtct
    17281 ataatagatg ttggtacagg tgctggtttt cctggaatac cattgaaaat atgtttagat
    17341 agcttagaac ttactttgtt agattcgtta aataaaagaa taaacttttt agaggaagtt
    17401 agtagagtac tagagttaga taatattaca tttatacatg gaagagctga agattttggt
    17461 aaaaatgaag actatagaga aaaatatgat gttgctacgg caagagctgt tgctggatta
    17521 ccgattctta tggaattttg tgtaccattt gtaaaagttg gagggtattt tatttgctta
    17581 aaaggtccga atgctaattt agagctagaa gaatcacaaa aggctattga tgttttagga
    17641 ttggagtata ttgaaaaaat agatgtagag ttaccagagg tagatttaaa tcataatata
    17701 ttagttttta aaaaggtgat ggaaacacca gttaagtacc ctagaaaagc tggtagacca
    17761 gctaaaaatc ctattaagta atgaaaaaag taatgaaaaa attattattg gtaagcttaa
    17821 ttatttctta ggaaataatt aagcttaccg taaaaaaaca aaaataaagg aattattaaa
    17881 gtagatgcag aaataaattt gtatgtacat atagagaaca aaaataattt atatctaaaa
    17941 taagagaggt atgttatgga agataaaaga gttatggaga tacctataga agatgtagtt
    18001 ccaaatccat atcaaccaag aaaaatattt tcacaagttt cattggagga attgagcaat
    18061 tctataaagg tatatggtat aattcaacct ataacagtta gagcaaaaga tggcaaatat
    18121 gaactaattg caggagaaag aagattaaga gcagctaaat tagcagagct taaaaccata
    18181 ccagcaatta taaataatat gaatgatgaa tcttctgctg ttctagcgtt attagaaaat
    18241 ttgcaacgag aagacttaaa tttcattgaa gaagcaattg gttatgaaaa cttaataaaa
    18301 gaacatgctt ttactcaaca gcaattagca gaaaaattgg gcaaaaatca atctactgta
    18361 gcaaataagc ttagaatatt aaagcttcca aacgatataa aaatgaagct aatagagaat
    18421 aatttaacag aaagacatgc tagagcattt cttaaactac caagtgaaga tttaatgcaa
    18481 agtgtgttag acaaagtcat aaaaaatgaa ttgactgtta aaaaaacaga aaaattgata
    18541 caagatgtat tagaagaaac taaagttcaa gaagagcctg ataaaaaaca gaatataaag
    18601 ggtgctatga gtataagaat ttacataaat actataaagc aggcctttga tgctatatca
    18661 aatacaggaa ttgatgctaa gtataatgaa atagataagg gagattatat ggaagttgtt
    18721 gtgaaaatcc ctaaaaaata gagattactg cttaccagta atcttttttt gtaatataag
    18781 ccttgtaata taaattgatt tatagacatt atttatactt aaaaaacacc tattcaacat
    18841 ttaaaattaa tttaattctt aaagggggga acatcatggg caaagttata gctgtattca
    18901 atcaaaaggg tggagtcggg aaaactacaa caaatgtaaa tttaagtgct agcttaggaa
    18961 ctttgggaaa gaaaatatta gtattagatt tagacccaca aggaaataca actagtggat
    19021 atggtattaa taaaaacgaa gtagaaaata caatctatga aattatgtta gatgggttac
    19081 atattaaaga agcaataata agtactgaat ttgagaatgt agatgtagtt ccatcagcta
    19141 ctgaactatc aggtgcagag atagagctta catctaaaac taatagagag tatattttaa
    19201 aaaattccat aaaagcggta atagatgaat atgattatat atttttagat tgtccaccct
    19261 cactggggat gcttacaata aattgtttaa ctgctgtaga tagtgtttta attccaatac
    19321 aatgtgaata ttatgcttta gaaggtgtta gtcaattaat ggagactata aaattagtaa
    19381 aatcaagatt aaatgctgat atagaaattc aaggtgttgt tttaagtatg tttgatggaa
    19441 gagctaattt atcaatacaa gtagttgaag aagttaagaa atactttaaa ggtagtgtat
    19501 atacaacttt aattccaaga aatgtaagac ttgcagaagc tccaagtcat ggaaaacctg
    19561 taatttatta tgataaaaga tgtagagggt ctgtagctta tttggagcta gcagaagaat
    19621 ttatagattt agaagaggag gaatggtagt atggagaata agtctaaaag aagtaataga
    19681 ttaggtagag gtcttagtgc attgatacca gaaattaaag gagaaacttc agaaaaagaa
    19741 atagtcaaca tagatataga caaaatatat ccaaacgaag ttcaacctag aaagcaattt
    19801 gatgaagaaa agataaaagt attgtcggac tctataaaaa attatggtgt tttacagcca
    19861 atagtggtta aaatggatga aaataataaa tatatgataa tagctggaga aagaagattt
    19921 agagcatcaa aacttgcaaa taaaaatcaa atacctgcta taattaaaga tatagatatg
    19981 aaagatataa tggagatagc cttaattgaa aacttacaga gggaagattt aaattcaata
    20041 gaagaggctc tagcatataa gagtttaata gagcattaca atgtaactca ggaagaaata
    20101 tcagaagctg ttgggaagag tagacctcat ataactaata cactaagact tttaaatctt
    20161 ggacaagatg ttatagaaat gatagacagt ggtagaatta cagcaggcca tggtaaggct
    20221 ttattgagaa ttgcagataa agatttgcaa cttcaaatag cgaaaaagat agaagaagaa
    20281 gaactttctg taagagaagt cgaaaatata gctaaaaaaa tatctgaaaa taaacaagaa
    20341 gaacctaaaa aatctaaacc taaggatgta tttattttag atgttgaaga taaacttaga
    20401 aatatatttg gaacaaaggt gaatatttct aaaggaaaga agaaaggtaa aatagagata
    20461 gaatactata atgatgatga tttaaatagt attgtatcaa tgcttcttga atagaacaaa
    20521 aatataaaac agagaggaag attgttatga tttatttaga taatgctgca actacatatc
    20581 caaaaccaga aagagtgtat aatgctgttt tagattgtat gaaaaattat tgtgcaaatc
    20641 caggtagagc aggccataaa cttgctatga gagctgctag ggagatatat gatacaagag
    20701 agaatatagc taagttgttc aatgtaagta atcctatgaa tatagtattt acatctaatg
    20761 caacagattc tttaaattta gcgataaaag gtgtattaca agaaggagac catgtaataa
    20821 caacgtctat ggagcataat tcagtaataa gacctataaa agcattggaa aaaaggggta
    20881 ttgaaaatac tgtagtaaaa tgcgattatg agggattttt agattatgaa gatttagaaa
    20941 aatcaataaa gagtaatact aaactgattg taacaacaca cgcatctaat gtatgtggaa
    21001 ctttaataga tataaaaaaa gttggagaaa tagctaaaaa acataatata ctatttttag
    21061 tagatgcatc acaaacagca ggtgtttatg atatagacgt aaatgaatgt aatatagata
    21121 tgttggcaat gccaggtcat aaatgcttat ttgggcctca aggaacaggt atattatatg
    21181 taagagaagg gttgaactta aatatattaa aagaaggtgg aacaggtagt aaatcagaag
    21241 aaatagttca accagagtta ttcccagata agtatgaatc aggaactcat aatactccag
    21301 gtatagcagg tcttaaccaa ggtatactat ttatatttga aagaggaatc aacaatataa
    21361 gacaacatga agaagaatta tgtcaatata tgatagacaa actagaggaa gtatcagata
    21421 taaagatata tggaccaaag gatagtaaaa aaagagcatc agtaatcgct ctaaatatag
    21481 gagatatgga ttctggagag gttactttct tattagatag tgattataat atagcaacta
    21541 gatcaggaat acattgttca ccactagcac ataccacttt aggtacttta aaacaaggtg
    21601 cagtaaggtt cagtatagga tatttcaaca caaaagatga gatagataaa gcagtggaag
    21661 ctttgaaaaa aatttcaaaa aataaatagc atgagattta ataatataga aataaaaaaa
    21721 ggggatattt caaaataaaa atttgaatca tctccttttt tgtacaaaaa agaaaagggg
    21781 ctcctaagaa cctctaatct atttgataat tgtttcaaga aattacttta agtaatctcc
    21841 ttcttcaaga actatatgac cactttcgta attagaacca tcaacagtaa tttttatttg
    21901 ttcaacattc atatttttta tgtaagtacg agcaactgaa tcaagcatta aagtttctgc
    21961 atcactacct aagtttgact ttgtaaagtc agagctaaca tctaatatac cagtttttac
    22021 accatcaatg tcttttgtag taaatgaatt taattttgca gattctggaa caactttaag
    22081 tttttgaagt tcttcaaata aagtgttctc atcaatttta tttaaatcaa ctttattttc
    22141 aattaaatct gtcttatcta catcaaaaga gtaaatagta gctgattgag ttttagtttc
    22201 ttcttttgga gtaggtttag ctttgttatt aggaacctct ttttcagtat tgttttcttc
    22261 ttttggttgt tcaacatttg tattgctctt agtttctttt ttagatgtat cacttccatt
    22321 ttgagcatta gtacaaccaa ctgcgaatat agatatagat aatatactta acactaacat
    22381 tattttctta tttttcataa aaatttctcc tctcttaaaa actattctta tatataatta
    22441 tagtcgttat gagcaaatat atgaatacaa aatagtaaca aatttaaaaa atgatttatt
    22501 taaattaata aaactcaaaa taaactaaat tttagtcatt tttatgggaa atcatttaat
    22561 gtacagtgaa aatattgtaa aataaaagta gtgtttttaa aataaatata agttataatt
    22621 atattataaa aaaatgtctt caaaaggtgg gaatagtatg gaaattttga gcttaggaga
    22681 aaagattaaa aaattaagaa aagaaaaaaa catgacatta aaagaattag ctggagatag
    22741 gataacagca gcacaaataa gccatataga gagggataaa tctcatacta gttatgaact
    22801 gttggaatat ctaggcgata agctagatgt aagtgtagaa tacttattag agaccaagga
    22861 aatgcaatca aaaaagataa ctgataattt aatactacag agcgaaattt atataaaaga
    22921 aaatgaatta aacaaggctg aggatcaaat aaaagaagtt ttagatatat gtaagagata
    22981 cgatttaatg gagaactatg gcagatgtaa ttttttaatg gctaatatta atttgaaaaa
    23041 atctaattat aatgatgcta tagaaaactt tgaaaaggca ttgtacttct ttataaaaaa
    23101 tagtgatagt gaaaatatac ttaagtgtta tttaaacata gggaaaatat atatgcagga
    23161 agacttttat aaaggtgcta taagccactt taattttgca gaggaaattt tgtcagagaa
    23221 taaatttgag gatgttaaca tacacaagga actatatagt aagatggcat attgttacat
    23281 aaaactagat aatccaaata tgtcattgaa gtacatagaa aagataaatg aaatagataa
    23341 tagaaatgat agaaaagaag atgtcgatat tttggtgtta aaagcaaata atatgcttga
    23401 gattggaaaa tatgaagaat caagagatta ctttaaaaaa gcactaaaaa tacttgagag
    23461 tgaggataat aaaactggat tagcaaatgt gtatttaaca atttgtgatg tttacagaaa
    23521 aataggtgaa acagatagag tacttgaata ttcacaaaag gtatatgaca taaagaaaaa
    23581 tgatgaagat gaatacatga tgtcaggatt atttaagata atagaagcat atatagataa
    23641 agaagattat gatttagcta gaaaatattg taagatagcc ttagcttcat caataaaaaa
    23701 taaaaataaa ttcaatgaat ataaagcatt gagattctat gctaatatgt ataaaaatca
    23761 gaatgaagta actcttgcaa ttgaatatct gattaaatgc ataaaaatag tatcagattt
    23821 aggcaacaat agaatattgg ccaatttata tatagactta ggtcaattat attcaaatat
    23881 atcaaaagaa aaagagttag aatattatca aaaaggtgta tttatgtaca aaaatttaga
    23941 aataatgtag ggtaaataaa cctataaggg tatagaaaca atctataaca agcaagataa
    24001 tatctatgat aaaatataaa ttgtatgaat atttttaagg agatgaatca gacaatgagt
    24061 ataaaaatag atgcgagagg gcttgcttgt cctaaaccag ttataaatac aaaaagagaa
    24121 ttggataacc tagaagaagg tgttgtagtt acaatagttg ataatgaaac agctaaggag
    24181 aatatattaa aacttgctaa atctttaagt tgtgaagcaa atattgttga cgaaaaagag
    24241 aattttatat ctatagaaat tagaaaaggt aaaaatatac tagataaaca aaatgttgta
    24301 gataataaag aaagtgaatt agacaataca tgtatattta tatcttcaga taagatggga
    24361 cttggaaatg acgaattagg aaaagtgtta ataaaaggat ttatatatac attaacagaa
    24421 tctaaacctt atcctaagta tgtactttta gttaatggag gagtaaaatt aagtgctgaa
    24481 aatgaagaaa ccatagaaaa tctaaaaata cttgagaata tgggtgtaga agttttatct
    24541 tgtggtactt gtttagatta ttataattta aaagacaaat tacaagtagg tacagttaca
    24601 aatatgtata ctatagttga gacattaaag aatgcatcaa ataccatttc tatataatgt
    24661 atgtttgttt aataatataa aactaataat aaagattaac tcaaatagat agtcagaaag
    24721 gattaagtaa tgaatcaaat gtatatagtg tcatttaatt cgacacatca tgcgataaga
    24781 tcagaaaagt tatttggtga aaatagtcta aaagtaatgg ctcttccaac accaagagaa
    24841 ataacagcaa gttgtggaat atcaattaaa ttttcttttg aagatatgga aaagataaaa
    24901 actattttgg ttgaaaataa tgtagatata aaaggaattt attgtataag taaattagaa
    24961 aatggttcaa aagaagtaga gaagttagat taggaggcta attatttatg ccaatggatt
    25021 taaaaatagg tgatattgtt gaattaaaaa agcaacatgc atgtgggtgt aaagagtttg
    25081 aaatcataag aacgggaatg gatataaaga taaaatgtac taaatgttca aggctaataa
    25141 tgcttgatag agagacctta gaaaagagag taaaaaagct aataaaaaag tgataaaaat
    25201 ttaacactta aaaaatgtca aataccgtat attaatatcc aaaattttgg tataataaga
    25261 attgaagaaa acattttcat gggggtgctt taattatggc agttaaatat gcaaaaagaa
    25321 tgcaaggatt acaaggatca gaaatacgtg agcttttaaa acttactcaa caaccacaaa
    25381 taatatcttt tgctggtgga atgccggctc cagagttatt tccagttgag gaaatgaaaa
    25441 aagtatcagt tgcagtgctt gaagaaaatg gaagatcagc tatgcaatat actacaacag
    25501 aaggatatga accattaaga gaaaaaatag cagctagaat gaatgataaa aacaaaacta
    25561 atgtaaacaa agatgatata ttagttacaa gtggttctca acaaggtcta gattttgcag
    25621 gaaaagtatt tattgatgag ggtgatgtaa ttttatgtga aagtccatct tacataggag
    25681 ctataaacgc atttaaatct tatcaaccta aattcataga tgttccaaca gattctgatg
    25741 gaatgataat ggaagaatta gaaaagatac tagaaacaac tgatagaata aaaatgattt
    25801 acgtaatacc tgacttccaa aatccaacag gaagaacttg gccacttgaa agacgtaaaa
    25861 aattcatgga aatagtaaat aaatttgaaa taccagtaat agaagataat ccatatggag
    25921 atttaagatt tgaaggagag actttaccat ctttaaaatc aatggataca aaaggattag
    25981 taatattctt aggaactttc tctaaaatat tctgcccagg atacagatta ggatggactt
    26041 gtgcttctcc agaaatttta tctaaattta actttgcaaa acaaggtgca gacttacaag
    26101 catcaactat atctcaaatg gaagtaagca aatttatgga tatgtatgac ctagatgcac
    26161 atgttgacaa aataaaagca gtttatgtta aacgtagaga tgtaatgctt aagactatgg
    26221 aagaagagtt cccagaagga ttagtattta ctcatccaga aggtggatta tttacatggg
    26281 tagagcttcc aagtaattta aatgctaaag aattaatgcc aaaatgttta gagaagaatg
    26341 ttgcttatgt tgctggagga ggattcttcc ctaatggtgg tagagaaaat actttcagac
    26401 ttaactattc aaatatgcca gaagaaaaaa taatagaagg tataaaaaat atagcagctg
    26461 tattaaaaga agctatgggt gtagaagctt aattaaaaat aataacttat atataataca
    26521 agggatggtt gttgccatcc cttgtttgtt tgaaaagtat ataattcacc ttatactaaa
    26581 ctttgtccaa aatttaaatt gacagaaata agaataaatg ataaaatgtg taggtatgag
    26641 attttagtct catttctctg ctctaagagt gtagagccgt taagtccaaa gggaggtgaa
    26701 ttatagtgag aaattatgaa ttagtttatg tggtaaagcc aaattctgat gaagaagtaa
    26761 gagaggctat actaaacaaa gttaaggaag ttgttgcaac tgacggagaa atagttaaag
    26821 ttgatacttg gggaactaaa aaattagctt atccaatagc taagtttaca gaaggtttct
    26881 atgtattagt taacttcaaa tcagcagtag acgttcctaa agagatagac agaaacttaa
    26941 agataaatga aaacgtaata agacacatga tagttgttgc ttaataactg gttttattat
    27001 aaaaaatagg tggtgttttt atgaatcaag ttgtattagt gggaaggttg actagagacc
    27061 ctgaattaag atacatccca ggtacaggaa caccagtggc atcctttaca atagccattg
    27121 atagagacta tgtaaaaaaa gatggatcta aagaaactga ttttatacct gttgaagtaa
    27181 tggggaaatc tgctgaattt tgtgctaact acataactaa aggtagatta gtagcacttc
    27241 aaggatctat aagagtagac aattatcaga cacaatcagg tgaaaagaga acttttacaa
    27301 aagtaagtac tagatcagta caagctttgg atagtaaaaa taaatcggaa aattcatata
    27361 gagagagtgc tccagctttt gagccaagct ttgaacctca aggtttagac ccacaaggtt
    27421 ttcaagctat agatgatgac gatataccat tttaaaaagg agggaaaaaa gtcatgatga
    27481 ataagaaaag acgtaagaaa aaaagagttt gtcaattttg tgctgacaaa aacgctaaaa
    27541 tagattacaa aagtactcaa agattacaaa agtatataac tgaaagagga aagatattac
    27601 caagaagaat atctggaact tgtgctaagc atcaaagaga attaacagtt gctataaaaa
    27661 gagcgagaaa tatagcactt ttaccatata cattagacta aaaaaatgag ccttgtgctc
    27721 attttttatt atatgtattt atatatctaa atgaggcgag ctataaagct cgtttttttg
    27781 tttatatact taaatatatt attatttatg atataatatt atatattaag gagggttgta
    27841 aacgtgaaaa tagataaagg ttctgagata acgagaaaca taaagataat agaatggatg
    27901 aaaacagaaa tattaatgag tgtaagtgat ttgtttaatt tattgtttaa aggggtaaaa
    27961 ccattagatg aagcaattca agatacttta gcaaatataa taatgataac atatctctta
    28021 gcaaagagat taggaattag ttttagagat gtggattata aagtcaagga aaaaataaaa
    28081 attggtatag atgaggacca tagcgttgaa agatggtatg gagatttatc taatttaaaa
    28141 aaacatatgg ataataggga gtgatattaa attgaataat aaaattagac tatctaaggc
    28201 aatgtctata atagttttag ctataataat cgctttagta atagcctata ttccaatgct
    28261 aggaatattt agtatgttag cagctgttcc atatgtaatt ataggtgcta ttactgatag
    28321 aaagtattct cttatatcta ttttggttac attttgtgta ctgattctat ttgcagacat
    28381 ttcttatgca ttaaatatat gtataatgta ttctttacca ggtatagtaa taggaaagat
    28441 gctaaagaga agctttgaac aagaggacag taataaattc gaaccgatat atggtggaac
    28501 cataatattt gtactttcaa tgcttgtata tttttttgta ttaaagacat ttttaaatgt
    28561 gaatctatta gatgaagttt ctaagatgat atcagaaatt gtaaatattc aaaaaaatag
    28621 tttttctgca actgaactta aagtgtttga taaaatgaag ccagatgaga ttgtaagtta
    28681 ttttacaaat atgctaccaa tgatgttatt tttacaaggg ttgttatcag cgtttgtcac
    28741 atattatttg agtgtatttt ttataaaaag gattacaaag atgaatataa gatttcctaa
    28801 atttgctgat ttttatttac caggaaatgc tatactcaca actttcttac tttatttatt
    28861 agtcttattt attcaaatta ttggttcaaa attatataca gaattaataa tgactaattt
    28921 acagctagtt tttaatttaa tgtttgtaat tcaaggtata gcagtttgca tttatttctt
    28981 aaagaaatgg ataagacagg gaccaaataa aattatgttt ttttcaggga taattttatg
    29041 tttatttgga tttatgggaa tatcttttgt tggtatggta gatagtataa ttgactttag
    29101 aaaggtaaga agctataaat ccacttagga ggatagaaat gagtaacaaa caaaccttta
    29161 aattaaatat gccagaaata aatttatata taattgttat tggtatttct agtataattt
    29221 tgctttatta caacttgtat gtagggtgtt tatttttctg tatttttgta tatatggttt
    29281 ttcacaattg gagaactact aatattagac gtcatgaatg gacagagtat attcaaaatt
    29341 tatctttaga tatagatgaa acaactaaaa aagccataat aaatcttcca ataccattat
    29401 gtattttaga atttgatgga aatatatcct ggtataatgg aaagttttat gatatgattg
    29461 gtcaaaaaga tttacttgat aaaaatatag aggatatagt taaaaatctt aatttaagaa
    29521 aagtattaaa tgaaaataaa gagatgtaca cagaaataaa ctataaagaa aaagaatata
    29581 caataattta taatgttata aaaaatgatc aagaaaaaaa tcctaaatat ttgatgatat
    29641 tatactggat agacaagact gaatatttaa aagtaaaaca aaattatgat gatgaaaaaa
    29701 atgcaatgat gctaatacaa gtagatggat atgatgaagt attaaaaagt gcagcagaag
    29761 ataagagagc tttaattaat gttgaagtag agaaaatact ttctgcttta gaattaaatt
    29821 caaatggtgc attgagaaga acttcaaaag acaaattctt cttggtaatg cataaaaaag
    29881 agctaaaaaa attggaagct gagaagtttt ctattttaga tacaataaga catattgatt
    29941 atggaaataa tcttcctgtt actataagta taggaattgg tatagatgga gatacattaa
    30001 atgagaacct aaaacttgct acgggtgctc tcgatttagc acttggtaga ggtggtgatc
    30061 aggctgttgt aaaaacaaaa gataaatttg tgttttatgg aggaaaatct aaagctgtag
    30121 aaaagaaaac caaagtaaaa tccagattaa taggtcatgc tttaagagag gttatacaac
    30181 aaagtgacca agtttatata atggggcata aatatcctga tatggatgct atgggagcag
    30241 cagttggtgt ttatgatata tgtaaatcct gtaacaaaac tgcaaacata gttttacaat
    30301 ctgtaaatga atctatagaa atatttataa ataaaataaa tgaaaataat tactataaaa
    30361 agctgtttat tggaaaagag gaagctattg acaactgtac taaaaataca ttagttgtag
    30421 tagttgatac tcatagacca aattatactg aatgtgaaga actgttaaag ctatcagaga
    30481 aggtagtagt tatagaccat catagaagag gtgtggagtt tataaatgat gcagtgcttt
    30541 tattccatga gatatatgta tcctcaacgt gtgaaatggt tacagagtta gttcagtata
    30601 tggatgaaga tgtaacaata aataaactta cagctgaagg gcttttagca ggtataagtc
    30661 tagatactaa aaactttgca ttcaaaacag gtgttaggac ttttgaagct gcttcttacc
    30721 ttagaaaagt tggagcagat actatagagg taaagaaatt cttcaattct gatgtaaaag
    30781 atttcataat aaaagctgaa ataattcaaa gtactaaaat aattaataat agaatatgtt
    30841 tagcatattc aagtactgag atagatagta taaatgttat aatagctcaa actgctgatg
    30901 agttattaaa tatcaaagaa gtagaagctt cctttgtatt aggtgaaaaa gatgatacta
    30961 tatttataag tgcgaggtct ttagggcaaa taaatgtaca tgtacttatg gaaaaattag
    31021 gaggcggggg acacatagat atagctggag cacagttaaa aaatgtttct ctaaaagaag
    31081 cttataagat ggtaaataaa ataatagagg agtatttgga ggaggaagaa tagatgaaag
    31141 ttatattatt aaaagacgta aaaggaactg gtaaaaaggg agaaatgaaa gaagtaagtg
    31201 atggatatgc aagaaatttc ttatttccta aaaagatggc tgtgcaagca gatagtgtag
    31261 caataaaaga attaaatgaa aagaataaat ctaaagaaat aaaagcacaa aaagagtatg
    31321 aagaagcagt tttattaggt aagcaaatgg aagaaattaa tatagaaata tattcaaaag
    31381 caggagaagg tggaagatta tttggttcaa taacttctaa agaaattgct gagcaattaa
    31441 agaaacaaaa agacatagac gtagataaaa gaaaaatatt attagatgaa ccaataagat
    31501 ctttaggttc aactttcgta gaaataaaaa tacatcaaaa agtaactact aaaataagag
    31561 tggatgtaaa agaaaaacag taataactag aggtgattaa gatggaagat atgacgagaa
    31621 ttcctcctca tagtgtagaa tcagaacaat caatattggg ttctatactt ctagataaag
    31681 atgctataat aacggttaca gaaactataa agcctgatga tttctataaa gaggctcaca
    31741 aaataatata tgaatgtatg ataactttaa gtaataaagg tgagcctata gacttgataa
    31801 cattaacaga agaactaaga aaacaagggc atcttaatga tattggagga atcagttata
    31861 ttacaagtct ttctactata gtaccgacga cttcaaatgt aaaatactat gctgatatag
    31921 taaaagaaaa gtctgtactt agaaagctta taaaagcttc taatgaaata ataaatctag
    31981 gatatagtgg agcaactaag attgaagatg ttttagaaca agctgaaaag agtatatttg
    32041 atatatcaca ggagaaaact agtgatgatt ttaaatctat aaacttagtt ttgatggatg
    32101 cctatgatat gatagaaaaa ttatatacta acaagagtga tgtcactggt ataactactg
    32161 gttttaaaga tttaaacaaa aaaataaatg gtcttcaaag gactgattta atattaattg
    32221 cagctagacc agcaatgggt aaaactgcct tttctttaaa cttggttcaa aatgcagcct
    32281 taaaaggtga tgcctctgta gctgtattta gcttagagat gtctaaggaa caattagtac
    32341 aacgtatgtt atcttcacaa tctagtgtag aattaaaaaa aataaagact ggaacactta
    32401 atgataatga ttggcctaga attatagatg ctatggcagt attatcagat gccaaaatac
    32461 acatagatga tactcctggt ataaaaatat cagaactaag gtcaaagtgt agaaagttaa
    32521 aaatagaaaa aggactagat ttagtgctta ttgattatct tcaacttatg gaaggtgaag
    32581 gtaataatga aagcagacaa caagagatat ctaagatatc aaggtcacta aaaatattag
    32641 caaaagaatt aaactgtcct gtagttgcac tatctcagtt atcacgtgca ccagaacaaa
    32701 gagcagacca tagacctatg ttatctgatt taagagagtc tggagcaata gaacaggatg
    32761 cagatatagt tatgttctta tacagggacg aatattatca tgctgattct gagagtaaaa
    32821 atataggaga agtaataata gcaaaaaata gacacggtga aactggttct gtggaacttg
    32881 tttggcttgg tgaagttcaa agatttgggg ataagttgag agacctataa tctaacgaat
    32941 atccacagaa cgtcctttct ttctacagga aaaatgtgga tacatgtcac taaatatgtc
    33001 caaacattga aatttagcta aaaaactatc cacactatcc acagatagcc tgttgataag
    33061 tgtgaataag tagataaagc aagcttttgg cttgctttat ctgtgtataa atattaatta
    33121 ttaggaggaa taatatggaa aaaataagct taaaatatat atatcccaat ataattaaag
    33181 tacttgatga aataaactta tttagagtaa ttgacaataa tttaagagag tctattgttg
    33241 tttatgctaa taatgtggat aatcagtatc atataaacat gactaataca aactttggaa
    33301 acataattaa catatgtaaa ttagaaaaac ttttagatgt ggataaattt atggaaaaag
    33361 ttataaaata tgaaaaagaa ataatagaga aagaagagtt ttcaaaaata gaagaatata
    33421 tgttaaatat tggagaatac taaaaaagtt gaaaaaaata cgaatgtttg atagaataag
    33481 taagtaaagt gttcgagagt atataaaata tagaggtgaa ttacatgaaa acagttgcaa
    33541 ttgttggttc tcaatgggga gatgaaggta aaggtaaagt tatagattat cttgctactc
    33601 aagcagatgt agtagtaaga gggcaaggtg gaaataatgc aggacataca ttagttgtag
    33661 aaggtaaaaa atacgcatta catctaattc catctggagt tttaaatcca aatacagtga
    33721 atataatcgg aaatggaata gtgtttgacc ctaaaggatt tttagaagaa ttggaaatgt
    33781 ttaaaacgga taatataagt acagaaaata taaaaataag tgatagagca catgtcatat
    33841 tcccatatca taaagaacta gatgcattgt cagaagaagc cagaggcgat ttaaagatag
    33901 gtacaacaaa aaaaggtatt ggaccttgtt atatggataa aacagagaga tctggtatta
    33961 gaatatgtga cttaatggat aaagataaat ttgctataaa attaaaagct caaatagatg
    34021 ctaaaaataa aatagttaaa aatatatatg gaaaagaaga attatttgat tttgaaacta
    34081 tatataacga atatctagga tatgcagaac aaataagaaa atatgtggca gatacgtctg
    34141 ttattgtata tgatgcagta agagcaggta aaaaagtatt gtttgaagga gcacaaggga
    34201 ctttattaga tttagattta ggaacttatc catttgttac atcttctcat ccaacatctg
    34261 gtggatttgc aattggtgca ggtataggac caaatatgat aaaagacgtt gttggtatag
    34321 taaaagcata tactacaaga gttggagaag gaccgtttgt tacagagcaa ataaatgaaa
    34381 ctggagataa gataagagaa caaggacatg aatttggggt aactactggt agaccaagaa
    34441 gatgtgggtg gtttgatgca gttatagtta agtatgcagc tagagttaat ggattgacta
    34501 gtatttcatt tatgttactt gatgtattaa ctggatttga taagataaaa gtttgtacgt
    34561 cttataaaat gggcgataag ataataacgg atttcccagc ttcattagat gatttagcaa
    34621 aatgtgaacc tgtatatgaa gaattagatg gatggaatga agatataact caaatagata
    34681 actttgatga ccttccagaa aatgcaaaga aatatgtagc aaaaatagaa gaactagttg
    34741 gagtaagtgt agatatggtt tctgttggtc caaatagagc tcaaactata ataagaagaa
    34801 atatatttgc ttaatatttt atcaattaaa taatttaaaa tagttgaaga agagtatctc
    34861 aagataatgt ttatagttat tagagatgct cttttatatt tgacttacat tgatttttta
    34921 ttaagttaaa gctacttata catattctag ctgaaattaa agttgaattt atgtagatac
    34981 atataggttt tttaaaaaga attttgttga aaataaatgg ctaaagatga ttttaattat
    35041 attctaaaaa aagtaaaaaa aactattgac taaaaattgt atagacgcta taatataact
    35101 tgtgagtgaa agaaacgacg aaaaaagtcg aaaaaatgtg gtctattagc tcagtcggta
    35161 gagcacctga cttttaatca gggtgtcccg cgttcgagtc gcggatagat caccaaaata
    35221 tggctcgttg gtcaagaggt taagacaccg ccctttcacg gcggtaacag gggttcaatt
    35281 cccctacgag tcaccagtat gggactatag ctcagctggg agagcacctg ccttacaagc
    35341 agggggtcac aggttcgagc cctgttagtc ccaccaaatt ttgaattaga gataaatact
    35401 agattatatg ctggtgtggc tcaacggtag agcagctgac ttgtaatcag caggttgtag
    35461 gttcgattcc tatcaccagc tccacaaagt acataaattc aaattaatat aattttctat
    35521 gttacaggat acttttaagt atcttttttt aatttcattt ttattgtatt aaaaatgaaa
    35581 ttaaacatac aacaaaattt tgaattactt gcaatgtgat agttttaaat ataaaattta
    35641 gaatcattct agcatagtat tatgtaatat agcaaattta atttatagta tcaataaata
    35701 tagactacta agaatgtaaa tgatttgtta gattaagata cattagatat gtaaatagaa
    35761 aaataaaaaa tatgttttaa tttatgtggc ataagtttta aacagagaag caagtttgct
    35821 cttacctctc tatttaaaaa taaaaaaatg ttttttaagg gctattttat ttaaatcttt
    35881 cccatctcaa atcatctcca gtaaattcta aaatcctaaa cttatctaag attctagaga
    35941 gagttctttg agtgtaagta tttcctattt gagatggtgt taagttggtt gaaataatga
    36001 tttttttacc agcaacaagt cttgtattta caatattgaa gatttcccca ctagtaaaag
    36061 aattattaag ttcagttcca aggtcatcta ttattaataa atcacaatca aacaagtttt
    36121 tataattatc ttcacttatt tggttatttg tgtctcttct aaatttataa tcttcaagaa
    36181 tatctaaaat tctaaaagaa gtttgataga ttacaacatt tcctttatct aacaattcct
    36241 ttgcaataca attgcacata taggtctttc ctagtccagt agaaccataa aacagtagat
    36301 tttcactatt atccttctta aaatcatgaa caaaattttc gcagatactt aaattattta
    36361 gcatattttc tctaggagat atttccccat cagagccttt cttaggagaa aatatattca
    36421 agttaaaatt cgaaaaattt tcctgactta aaatcctatc taaattagac attttataag
    36481 cttcatttac aatttcttgt ttgaggcaag aacatttttc tccattaggt aaaaatcctt
    36541 tatctttaca agaaatacat tgaaacttta tttccaagta atcaagaggt atgttacttt
    36601 cagataaaag tttttctttc tttactttta aagactctat attctcttta gtcttcttta
    36661 caatttcatc tttagatttg ggatttaaaa gtactatctt agctaaactg agacctattt
    36721 tagaaatttc atcatctata gattttatct cagggattct attatatacc tcattttttc
    36781 tatgttctaa taagagttca ttatcatctc ttctcttagc atatttagca agtatttttc
    36841 ttattttatc ctcattcatt taatcaccta cttaaacttt tctttttgac tttttttaat
    36901 aatctcatcg agctcatcag aggtatactg agtgaatgtt tcgttgaaat tatgaaactt
    36961 agtttttttg taagtattgt tgttttgagt agtattaatc tgcttcttat taatattctc
    37021 cttaactata cgttcttcct ctttttgctt taaatcattt aaatttttta tatttttttc
    37081 attccaattt ttaattatac catttatata agatatactt gggtttgaaa tattcttaga
    37141 cttagaacat gcatttatta tcaaatctat atccatatta aatttatcaa accaggtgtc
    37201 cataagttct ttttctgatt ttgatggttg tcttgaaaat cccaattcat tgaaaatcgt
    37261 tttatataaa atatatcgtt cacttcttac caaaaagctt tcttcaacat cttttggcgt
    37321 atataaattt gaatcatacc aatttctaat aatgccttca atgtatttaa ctggctttga
    37381 tgtaccagtt ttatctttga cgtattcata tgcgcaaagt atcatatcgg ggctcatatt
    37441 gtatttattc attatatcca taatactcat cttttcactt gggtcgagat atcgcccaac
    37501 tattttattt atagaattaa acatttttct aatacttgga ttttcagaag tagatacaac
    37561 cttatctgta ttagatttta tagatgaatt attgcttaat atattctcaa tatagaaatt
    37621 ttttaaatct aaaaattcta ttgaataatt aaaattatca tattctccat tgtcatgtat
    37681 ttttataatc ttcttttctt cccagaattt ccaagcagat aacacatcag acaatggtat
    37741 atttaggttt ttggctattg aattattgtc gaacttagga ttagaagtaa tatcacatgc
    37801 ttgtctatat cctaaaagat aaacttttac gtataacccg tccgccattg gcataaatat
    37861 atctataaag atattgggaa tagtgatttc acctaaatct atttcattgc tttctttaaa
    37921 aaacatttta tcacctcaga ttaaattata tcatttttta tattatattt gtctgtaaac
    37981 aaataaaagg tcacaagatt agcatgtaaa tattttctaa aagtattcat tttgaaaagg
    38041 aggagatatt ttggttataa aattcaacca aaaagtaaga agaacattat cagtagcgat
    38101 tctatttatg gtattaataa taggttgctt tatagaaaag gatgacagtt tagaaacata
    38161 caaagaaaaa actaattcaa aaataaatca gtataatatg gatgttatat ttgatgatga
    38221 gactaagagg ttaatgtgca atcagaatgt tgactacata aataatacaa aatcaaacat
    38281 agataaaata tatttccata tatatccgaa tgctttttcc aagaaagatt ttgctccttt
    38341 tgaaaaaagt gaaatgacaa gggcttatcc taatggattt aacgaaggat atatagatat
    38401 aaagaatata ttaaataata atagtaaaat ggagtataaa attaaaggag acaaaaatga
    38461 catattagaa atagaattag ggaaagagtt aaaaccaaat gaaagaatgt cattagatat
    38521 aaaatataac gtaaaaatac caaattcaat tggaagattt ggatatggag aaaatactat
    38581 aaatgttact aattggtttc ctatagcttg tgtaaatgat gaaaggggat ggaatttaaa
    38641 gagttatgaa actattggcg acccatttta tagtgagact agcgattttc atgtgaaact
    38701 attgattcca aataaatata aaattgctca tacaggaaaa cttattgatg ataaacatga
    38761 taataagaaa atgctatatg aaatagaagc tgaaaacgtt agagattttg catttatact
    38821 aagtagtaaa tttgatgtaa acaaagtaga ttcaaaggga gtagctatca atacatataa
    38881 tttaaataaa aagctttcta aaaaagcaac tgaagtggca aaagattcaa taaatatatt
    38941 tagtgaatta tttggaaagt atccttacaa agaattttct gttgtagcaa gtgacttttt
    39001 tatagggggg atggaatacc ctatgcttgt tatgatagac caaagtttat ataatgaaaa
    39061 aaatgaattt ttactagaat atgttattgc ccatgagaca gcacatcaat ggtggtattc
    39121 tgctgttgga aatgatgaaa taagtgaacc ctggttagat gaagcactta ctgaatattc
    39181 tacaatagtt tattttgaac aaaagtatgg taaagatatg gcaaacaagc ttataaaaac
    39241 tatggagata cagacaaaaa gttatttgag tgaaaatata tttaagccag caaatgagta
    39301 taaaaattct actgaataca gcttgaatgt ttatacaaaa ggcgcaatag catttaatga
    39361 aataagaaaa gaagttggag ataaagtatt ctttgagact ttaaatgaat actataataa
    39421 atataagcat aaaaatgcta atggaagagc ttttgtggaa ctatggaata gtaaaggtgt
    39481 agatataaat aagattataa gtgaatgtaa gtaaatagtg aatgtaagta aattttgtgc
    39541 agagtattaa attttattta atactctgtt tttttggtat aatataattt ataaggcaga
    39601 aaaagaggtg aaggaatgct aaagtattgt tcagtaggga gtggtagtag tgggaattgc
    39661 cactatatag ggtataaaaa tactaatata ctagtagatg caggattaag tggcaagaga
    39721 ataactacag gtcttaaaga cattggtgta gatgcagata aattaaaagg aatatttata
    39781 acacatgagc atgttgacca tataaaagga gcaggtatat tatccagaaa gttcgatata
    39841 ccagtatttg ctaatataaa aacatggtgt tctatgaagg acaaattagg tgacatcaaa
    39901 gataaaaata tgaaagtatt tgaaaatgat aaaacctact cattaggtga tatcatagtg
    39961 agaccttttt caatagaaca tgatgcgtct gatccagttg gatataattt ttatacagag
    40021 agtgatgaaa aaatgtctat agctactgat ataggatgta taactcaaaa tataaaaaaa
    40081 catctatata aatctaaatt agtagtattg gaatcaaatt atgaccctaa tatgcttatg
    40141 atgggttcat atacatatgc tttaaagaaa agagtaatgt caagtactgg tcatttatcc
    40201 aatgaagatg cagctaattt ctgcgttgaa cttattaatg aaggtacaga atctatatta
    40261 ttagcacatt taagtaaaga aaataatttt cctgagttag catatgagac ttcaaaagga
    40321 gtattagcat caaatgatat agtagttgga caagatgtaa aactagatgt actatcacga
    40381 aatgatgttt ctaatgttta tgagatgaag tagaggtgaa taactttgct aaagagaaat
    40441 tttgaaaatg ttgaggataa taaaatttta tactctataa agcagggtat gattttagca
    40501 atacctgcta ttatgactgg ttcaacggct cttgtaattt tgaatcttcc aataaaggct
    40561 tatcaagaat atttatcagg cttgtttaat ggagaggtta caaatattct taactttata
    40621 aatgattcta ctttaggaat tatttcatta ataatattat taacaataag ctatagttat
    40681 ggaaaaatat atgggagcaa gtatacagta cttgttccta tagttgcaat gtgctctttt
    40741 ttagtatttt cacatggtaa tgagcttaat tcttatatag aaatatttaa gacaaagtgg
    40801 ttatttacat ctataattgt ttctatgaca tcttctgtat tgtttgtaaa acttacagaa
    40861 tcttttgtta caagtgtaaa gtttcataca gaaggggctg atgcagactt taatatggta
    40921 gtttcggcta tagttccttt cataatagta gtatttttgt tttctatttt tagagtgata
    40981 atgataagtc taattggcag ttcaaacttt caagatatat tttataattt attttcaaat
    41041 gtttttaata aaatgggaac aaaccttatg agtgctctgt tgtttatttt tttaatgcac
    41101 tttatgtggt tttttgggat tcatggtagt aatgtattag ataccgtcgc aaaaaattta
    41161 tttgaaaata gtatggcaat taacataaat ttagttaata ataatcaatt accaacagaa
    41221 atttttacta agaccttttt tgatacaatg gttttattgg gaggatgtgg ctctttacta
    41281 tgtttagtaa ttgcaatttt tcttagtgaa aaacgtataa atgtaagaaa gctagctaaa
    41341 attgcttcaa taccagctct ttttaatatc aatgagatgc tagttttcgg gataccaatt
    41401 gtttttaact atattatgtt tgtacctttt gtaattactc ctataatctt aaccattaca
    41461 agttatgtgg ctatgtctac aggtattgta ccatgtacag tttcagctgt agaatggacc
    41521 tcacctatat ttttaagtgg atatatgtct acaggttcaa taagaggtag tttgttgcaa
    41581 gtatttaatt tatgtgttgg tgttatgatt tatattccgt ttataaaaat gtcgcaaaat
    41641 agatatacat atatgtttaa gaataatata gaaaatttaa caaacttatt aaaaaggtat
    41701 gaattaacag gtgaacagcc taatcttcta aaccataatg gtaaaatagg ttcaatttca
    41761 aaaatgttgg catcagactt gaagtttgct atgaaaagag gagaaataga acttttttat
    41821 caaccacaag tcaattataa tggaaatgtt gtgggagcag agggattact tagatggaaa
    41881 cattcaatag gagggttcat ttatccacca ctagttataa ttttggctaa agaagaaggg
    41941 ttcttagatg agcttggaga gtatattatt aataaagcat gtattgattt aaagaagagt
    42001 gaaaagctat tgagaaatcc tgttaagttc tctgtaaata tatctccaga ccaacttgat
    42061 gaccctaagt taccggagca aataaaaaat atcattagta gtaatgatat aaatcctaat
    42121 atgttaggta tagaaataac agagcaaatt gcattatcag gttcacaaat aattattgaa
    42181 cgtatcaatg cgatacataa cttgggaata aagttaatta tggatgattt tgggatgggg
    42241 catagctctc ttatatatct tcaaaataat aactttgata tagttaaatt ggatggttca
    42301 ttagttaaag agatacttac aaataaaaga agtagtgaaa ttataatgac tattgtaaat
    42361 ctttcaaaaa atttagattt tgatatagtt gttgagtatg ttgaaaatat agaccaaagg
    42421 gataagttat atggtttagg atgtgaaata tatcaaggat attattatag caaagcaata
    42481 ccttttgaag agtttgtaga atatgcaaat caagaaaaag agatataatt ttataagagc
    42541 ttgtaaattc tagtaatgca tagatttgta ataaaattaa gtttaggaga aatatatgaa
    42601 tataagtata gtagtagtag gtaaaattaa agaaaaatat ttaaaattag gtattgatga
    42661 gttcaaaaaa aggctttcta agtattgtaa gttagaaatt atagagttgg atgatgaaaa
    42721 agctccagag aatttgagtg ttaaggaaat ggaaattata aaagacaaag aaggaaagaa
    42781 aattttaggg aagataaagc ataatagtta tgttatagtc ttggctattg atggtaaggg
    42841 tttatcctct gaagaacttg ctaagactat gagtgattta gcagttagag gtaatagtag
    42901 tctctgtttt ataataggag gttctttagg gctttctgat gaggttttag gaagggctga
    42961 ttataggtta tccttttcta agatgacttt tcctcatcag atgatgagat tgatattgtt
    43021 agaacagatt tatagggctt ataggataaa taatggagaa ccgtatcata aataaatgat
    43081 acggtttttg ctaatttgac acttaataat attaaaataa caaaaaagta ttgaaagtat
    43141 attgcattta aggtagaatt aaataaaata ttataacatt ataatgttta tatagttgtt
    43201 aataaaaatt tggaggaaaa tatatgataa aaataatgtt ggcctgttca gcaggaatgt
    43261 ctacaagttt attagttact aaaatggaaa gtgcagctaa agaaaatggt atagagtcgc
    43321 aaatttgggc aataccagaa tcaactatac aaaatgaaat agaaaaatgt gatgtattat
    43381 tactaggacc acaagttaga tatgtattac ctaaggctca agaaattgct aagccatata
    43441 atataccagt agaggttata aatatgatgc actatggaac agttaatgga gaagctgttt
    43501 taaatagagc gatagaactt aacaacacta agtaatataa attataatgt atttttaata
    43561 tataaattgg gagaatcaaa ggatggatga aaagattata gaaatatctt ttaatataat
    43621 aggttatgca ggtgaaggta aagggttagc ttttgaagca ataaaagagg ctaagaatgg
    43681 aaatatagaa aaagcaaaag aattattaaa agagtctaag gaagttataa ataaagcaca
    43741 tagatatcaa acagaattaa tacaaaacga agctagtgga aacaggactg aaataagtgt
    43801 catacttata catgcacagg accacctaat gaatggtatg aattttcaac agttagctga
    43861 agaaatcgtt gatttacatt tgaaattaca agataaataa aaattaaaga gaataaatta
    43921 agagagttct taaaagatac attatgtgta gcacactaat tgttagatta ttgattcaat
    43981 atttagtgag taatacatta ggattatttt gaggactctt tttatattta gtttaaagtt
    44041 aataaatatt tattattaat aatataagat tatatgttat aatattataa catataattt
    44101 aagaggtgaa tcatgttaag aaatcaacca ttatatatac aagttgtaga tatattaaag
    44161 caaaaaatat taaataacga atataaaatt gatgaaaata ttccaacaga aagagaatta
    44221 gagcaagaat ttggagtcag taaaattact attagaaaag ctattgagat attagaatca
    44281 gaagggtatg tagagaaaaa aagtggaaaa gggacaaaag taataagtaa tagtcttttt
    44341 aataagctat caaaagcaga gtctttttca agtatacttt tatctaaagg tcataagtta
    44401 tctaaaaaga ctatttcaat tgaaaaaata ataaatgata caaattcaca agtatttagt
    44461 atatttggag aaaaatgtag caagattact agaatgtatt acttagatag taagccatat
    44521 atatttttta ctcattatat tccttttgat gaaaatctaa ctgttgaaag ttttaataat
    44581 gaaagttctt tatatatgta cttatatagg agaaatataa atataactag atttgaagat
    44641 gaatttttta tagaagaatc agaactagag gtatcaaagg ctctaaatct agctaaagac
    44701 atattattag gaagaaaaag aaagacctat gatgagaatg ataatatagt tgagctatca
    44761 tttgcaagat ataatacaga agtacaaaac tacgtaatta aatacgaaat ataataagaa
    44821 tcctgagttt attgaaatga taatatgctc aggatttttg tttagttgta aaaacgaaaa
    44881 atattataac attataatgt tataatattt ttaataaata agtgatatta tcaattacat
    44941 aaaaattagt ttatgttatg aaatcgttat tgcattataa ataaaggata atatcaataa
    45001 tacaggggga taactatgga aaaattcgca agttttttag agcgcaagat tatgcctgta
    45061 gcagcgaaga taagtaatca gagacatatg agagctgtaa gagcaggtat aatagcaaca
    45121 ttaccactaa caatagtggg gtcatttttc acaatattat taaacatacc tattgatgct
    45181 tatatggatt ttatagctcc atttaaagca acattagata taccatttag atatactgta
    45241 ggatttttat ctctatatgc tacttttggg atagcatcat cacttgctaa aagctacgat
    45301 ttagatacaa caggtgttgg tatgcttgca gttatggcat ttttagtttc tacaatagtt
    45361 ccaactcaag tagttgaacc agtaggtgga gttatagaag ctggtagatg gatggatatg
    45421 gcaaaactta attcgccatc attatttgga gctatcgtaa catcattaat tacagttgaa
    45481 atatatcgtt ttatgaagga aaagaatatt acaattaaga tgccagcagg agttccagta
    45541 gaggtttcaa attcatttac tgcattatta ccgacagcag taattttaat gttattctgg
    45601 gtaataagac atattttagg atttgatata agtgttgcac taagcgctat gttatcacct
    45661 ttaaaaggat tcttatcagg aaatagttta ggtggtgggc tactgacagt attcctaata
    45721 actttattct gggtacttgg gatacatggt ccagctataa tggggccagt aataagacct
    45781 atatgggaca tgtctatagc agaaaatata actgcattca cagaaggagt atcagctcat
    45841 gcaatgccaa acatatttac agaacaattt ttacagtggt atgtatggat agggggagca
    45901 ggggctactc ttgcattagt tgtaatgttt atgttttcta agtcacaata tctaaaaagt
    45961 ttaggtagat taggattttt accagggtta ttcaacataa atgaacctat aatatttgga
    46021 gcaccaatag ttatgaatcc aatactaggg ataccattta taattggacc tataataatg
    46081 acaattctat catatgttct tactattaca ggggttatac ctatgatggt agcaagatta
    46141 ccatttgcaa tgccagcacc aatagcagca gtaatgagta ctaactggag tatactagca
    46201 ggtatcttag tattaatcaa ctttgtgata tcatttgctg tttattatcc attcttcaaa
    46261 gtttttgaaa aacaacaatt acaaagagaa caagaagaaa tgagtgaaaa tttataaaaa
    46321 tacatagata tagaggtttt gtactatata aaatatagaa aaagcttata agttattaag
    46381 ggtgaaggta ggaggaatga cctttgcctt taattttata tattttaaat aatgtaattt
    46441 gaggaggaga aagatggaaa gaagattagg aatatcagta tatccagagc atagtaacat
    46501 ggaagatgat aaggcatata taaaaatggt ttcaaaatac ggatttaaaa gaatatttat
    46561 gtgcatgctt tcaattacaa gacctaagga agaagttaaa aaacatttta aagagattat
    46621 aacctatgca agagaattag gatttgaagt aatccttgat atagctccaa acatatttga
    46681 tatattacaa atatcttatg atgatttaac attctttagt gagttaaatg ctagtggaat
    46741 aaggcttgac tatggatatg atggaagtaa agaagctatg atttctttca atccatatga
    46801 tttaaaagtg gaactaaata tgagtaatga tgtagcatac ttggataata taattactta
    46861 caagccaaat gtaaataata tatatggttg tcacaatttt tatcctcaaa ggggtacagg
    46921 cttgccatat gatttttttg taagatgctc aaatagattt aaaaagtatg gaataagaac
    46981 tgctgctttt gtcaattcaa aatacgcaac aatcggacct tggaatataa atgatggact
    47041 gtgtacgctt gaaatgcata gaaacttaga tattgctaca gcaactaagc acttattcgc
    47101 aacaggattg attgatgatg taattgtagg aaatgcatat gcaagtgaag atgaattaaa
    47161 aagaatgtct gaagtaaatg aataccaagt tgtatttaat gtagtagcta aagaaaaaac
    47221 ttctgaagta gaaaagataa taatggtaaa tgagcaacac tttagaagag gagatataac
    47281 taatcaggta gcaagatcta cagaagtaag aaaaaaatat gctaaagaaa atttccctaa
    47341 acatgataat aagaacatct ttaaaagagg agatgtagtg attggtaatg atgactttgg
    47401 aaagtataaa ggggaattac agttgatact tcaagattgt acagatgaaa gaaaaaataa
    47461 ggtgggtaga ataaaggaag acgaaataat acttttagac tttattgaac cttggagtaa
    47521 atttaagatt attttagagg gagagtaaag tgaagataga catattaata aaatgtggaa
    47581 aaacagtaga taaaagtata attgatatag ctatattaga cgataaaata atagaagtaa
    47641 aaaatcatat agatgattct aagtatcagg caaaacaaat tataaactta gatggagaga
    47701 aatttattag tgcgggttgg atagatatac atacacactg ctacgaaaga ctagaccttt
    47761 ataaggatta tccagatgag gttggaataa aaagtggagt tactactgta gtagatgcag
    47821 gaacaacagg agctttagat ataggtgaat tttatgaaga tactaaaaag tataagacca
    47881 atgtatatgc tttaataaat attgctaagc agggaataac atctcaagat gaactttcaa
    47941 atatgatgaa tattgatgaa tatgaactta aaaatgcagt tagaaaatac aaagattttg
    48001 ttgttggaat caaagctaga atgagtaaaa gtgttgtaat ttcaaatgat gttgaacctt
    48061 taaaagtagc taaaagaata aaaaatgaac ttaatctacc tatgatggtt cactttggga
    48121 gttctcctcc aacgatagag gatatatttg attatatgga aaaaggagat atattaaccc
    48181 atatatataa tggaaagcca aatggaatac ttagaggcaa tgaggttaaa aaggaaatca
    48241 tagaagctaa agaaagaggt ataattcttg atgtaggtca tggaaccgaa agttttagta
    48301 tggatattgc aatgaaatct aaggatgcag gaatttttcc agacactata agtacagata
    48361 tatatattaa aaatagaata aatggtccag tgtataatct atctacaaca atggaaaagt
    48421 ttatatatat gggctattca cttgaagata taatagataa agttactaaa aatgcagcag
    48481 atgcaataag tttaaaaaat aaaggtttaa taaaagaagg atatgatgct gatttaacta
    48541 tatttgatgt ggttaatgaa gaaaaagaac ttcaagattc tttgaataag tcagtaatca
    48601 caagtacgtc tataaagcca agagcagtag tagtcaatgg agaatattta aatataggca
    48661 actctataga agagaatgag tagaagggag aaactattgt atgagtagta tatttgaaaa
    48721 atactctgta agacgtgtta taaatgcttc aggcaaaatg actatactag gagtatcaac
    48781 agtagataag gaagttattg aagcaatagg tgaagggtgt aaaaatttcc ttataataga
    48841 agaacttgta aataaaacag gtgaatatat agcaaattta ttagggtgtg aaagtgcact
    48901 aatagtttct agtgcatctg ctgggattgc acagagtgtg ggagcaatga tagcaaaaga
    48961 tgatatgagt ctagtttaca atgtaaataa tcctaagaaa caagttaaaa gggaaataat
    49021 aattccaaag gggcataatg ttgattatgg agttccagta gaagtaatga taagacttgg
    49081 aggaggagaa gttgttgagg ccggctatgc taatatgtgt aacaaggaac atataataag
    49141 agagataaat gaaaatacag cagcaattat gtatattaaa tctcatcatt gtgttcaaaa
    49201 gagtatgcta agtgtattag atgcagtaca agttgctaaa gaatataatt tacctttgat
    49261 agtagatgca gcagcagaag aagatttaaa tgtatattat gaattgggag cagacctagt
    49321 gatatacagt ggtgctaagg ctatagaagg tcctagctcg gggttagtaa taggtaagaa
    49381 aaaatacata gataatgtaa agcttcaatc aaagggtata ggaagagcta tgaaaatagg
    49441 aaaagaaaat attgtaggtc taacatgtgc aattgagaga tatataagta atgataaagt
    49501 aactttaaaa gaaatggagg acaagctaaa tccatttata gacaagataa atactataaa
    49561 gggagtgagt gctagtataa ctagagattc agcaggtaga gaaatacttc gtggagaaat
    49621 taactttaat gaagatataa taaataagtc aacacaacaa attataaatg aacttagaag
    49681 tggagagata gctatataca ctagagacta taaggctaat gaaggcaaga ttgagattga
    49741 tataagaagt gtaactggaa gtgatttaga taatatttat aataggataa aaaatatagt
    49801 agaaagttag agtgtagagt aagtgacatt ataatgattt aataaattga attttaccaa
    49861 tggaggggat aaatatgaat tgtataccac aatattataa aaatagagtt tgtttaaatg
    49921 ttcttgcagg aagcataaaa aatgctaaag aagtatatga tgcctgtgat ggacatgtac
    49981 tagtaggagt actatcaaag aattatagca ctgtagaagc agctattgaa gatatgaaaa
    50041 aatactcaaa ggaaatagat aatgcaatat ctgtgggtct tggagctgga gacccaaatc
    50101 agtcaaatat ggtatctcag atttctaaag aacttcagcc acaacatata aatcaagtgt
    50161 ttacaggtgt cgctacatct agagcattgc ttggtcaaaa tgatagtata ataaatggat
    50221 tagtttctcc aactggtaaa ccaggatttg taaaaatatc cacaggtcca ttaagttcaa
    50281 aagaaaaaga tggtatagtt cctatagaaa cagcaatagc tatgatgaaa gatatgggag
    50341 gaagctcaat taagtttttt ccaatgggag ggcttaaaac aaaggatgaa tatatagaag
    50401 ttgcaaaagc ttgtgcaaag cataattttt atttagaacc aacagggggt atagacttag
    50461 ataacttcaa agagatagtt caaattgcac ttgatgctgg tgtagaaaaa gttatacctc
    50521 atgtttatac atcaataatt gataaagaaa caggtgatac gaaagtggaa gatataggag
    50581 tactttataa aataatgaaa gaattattgg gatagattta tgaatatatt agcttttggt
    50641 gaaataatga tgagactttc agtacctgac tacaagttct taacacaaac caatgaacta
    50701 aattatataa ttacaggcac gggattaaat atattatctg gacttaaaaa ctttggatac
    50761 aatacctata tgttgacaaa gttgcctaat aataatgtag gtaaagcatc aagtgcaaat
    50821 ataagaaagc ttggagtaaa agatgacttt atcacatatg gagggaatca tataggagtg
    50881 tattttcttg aaaatgggta tggagaaaga ccttctgaag tgacttattt gaataggtta
    50941 aatagttcat tttgtgagag taaattacaa gattatgata ttgataaatg tttagaagaa
    51001 atggatatag tccatatttg tggtattgct atgcaactta cagattcagt taaaaagatt
    51061 gctttagaac tagctagaaa atcttatgaa aaggggataa aagtattctt tgattttaat
    51121 tttagacaat cattaaatcc agaaaggact tatgatgatt tagtaaatga ttataagaaa
    51181 atactaccat attgtcatgg gctgtttgct agttatagag atataaataa gatacttaat
    51241 atagatagtc taaatataaa atctagttca gaattgatta aaaaagagta tgatatagat
    51301 attgtagctg gaactatgag aagatttgat gatatgaata atagatttat aaagggatat
    51361 atatcaaatg atgatggtta ttttgaaagt agggagtata agttagaaat atatgacaga
    51421 gttggtgctg gggatggata tgtggcagga attatatatt catatttgga aaatatgaag
    51481 agtgaagata tagttgagtt tggggttgct agttcggtgt tagctcatac aacttatggg
    51541 gataattctt tagtaagcac aggtcatgta cttagactga tgaacaatga aaaaatagat
    51601 atattgagat aatacatttg tatataataa aatttttatc ttttttagac acacttttga
    51661 cttgcttata tttgttaaac agagttattt ttaacgatta taaatattaa attaaaagtt
    51721 agataataaa atagtagaaa atagcattaa ccatataaaa atggttaatg ctatttttat
    51781 agttaatatt gattaatttt atattataga aatgaacttt tctaatgctg gatttgtatt
    51841 ttttttattc catacaacca caacttcaat tctattttct acttctttta taggtactat
    51901 tgcaatatca cgttctatca ttgtatctgt aacacaaaat gaaggtaaaa tggatattcc
    51961 catgttagaa gataccaatg attcaatagt attaagatta tttgaacgtt gtaccacatt
    52021 aggtacaaac cccgcttcac gatatctatt ttgtatcatt gacttttgtc catactcatc
    52081 ccctgtttct tttacaaaaa taaatttatc atgttgtaat tcaattaaat cgatagaatc
    52141 ctcattgtaa agtggatgac caggaggtaa aagagctacc aaagaatatc tttctacagt
    52201 tttatattca aaatctttta atggttgatt ttctggatta ataacaaaag cgaggtccat
    52261 agaattattt tttactaagt ttaaaagttc ggttatttca ttagtacaaa tatccataga
    52321 tatattaggg tatgtattag aaaattcttt taagtattgt tgaagtttgt ttttttcata
    52381 tcctattaag gttccaatgc tcaaagttcc aaaaaaacca tgtgcaaatt gttgtgtttt
    52441 tgcaatagca tcattgatat tttttattat agtacgtgcc tcagataaaa aaacttttcc
    52501 ggctggtgtt agttcaacat gacgtttact tcttgtaaat agagttacat ttatttgttc
    52561 ttctaaagct tttatttgct gtgtaacagc agtttgagaa atataaaatt tttctgctgt
    52621 tttggtaaaa cttagatttt ctgctacaga tataaaatat tctaattgtt tagtattcaa
    52681 ataatcaccc ctatttaaac ttgattgttt gtaaacctta tttatctata attaaagctt
    52741 attatagtat aagaaaattt acttttaaag aatattagaa aatatttgta taaaaatata
    52801 tgttttaagt atatctttac attagatttt ataaatttat taataagaat aacttatata
    52861 ttccaatgaa aacgatattg gatttaagta agtttgtctc ataaaatgaa agtatatcaa
    52921 ttgataatac tttaaataag aagggagaaa aaatggaaga taaattttat gcaaaaggca
    52981 acggaaataa cggatatatt aaaaatcttg aagtttgttc ctttaataac ttagatggaa
    53041 cttgtggaat gtttcaaatg gctctgtaca aaagagatga aaaatactat ttatatggat
    53101 gctgttttgg aggaaataaa aaaaatggag taatgattag cgatattaca gacccttata
    53161 atccacaatt tataaaacat tttcaaatgt tagaccctaa agagtatcct acaacaacaa
    53221 ctccaaaaat tcaaatagca gatgatttaa tgatagtagc aatgagttgt ggaagtggac
    53281 caggagcact tgttgaccaa gctaaattag caaatattaa gtgtgaagca ggaattagaa
    53341 tatacagttt aaaagaagac cctttaaatc ctaagttttt aggatattgg gattgtggct
    53401 taaagcatgt aatgggtgtt catagattca tgtacaatgg tggaagatat gtacatttat
    53461 caagtgattg tgttggcttt gaaggtctga tttatagggt catagatata ataaatccta
    53521 ataatccagt ggaaataggt aaatggtgga gaccagacca atatgcagat ggatatccaa
    53581 atagaacttt tgatgcagga gcacctcatt gcccagaatt tatggataaa ggatggcttc
    53641 atggacctcc atttgtaaga gacggaaaag catattgtgg ttatggagga gctggtttag
    53701 ttgtattaga tgttgaagat ttaacaagac caagatgctt aggtgaattg ccatttatgc
    53761 ctgcattttc tagtagactt gcaggtgcaa gaactcatac agcattacca ttgccaggaa
    53821 gagatttagt cgttgttcaa aatgagggag aaagattcca gttctttaaa ccagataaca
    53881 ttacagatgt tcaagctatg aataatatac atatggttga tgttagtgac ccaacaaaac
    53941 caacattaat tgctcaattc ccatatcctg aagttccaaa agatttccct tatcctaact
    54001 ttaatgttgc aggattagga aaagcagggc catttggtcc acataatctt catgaaccaa
    54061 tggataataa gccatggtta gagcaaagag gagatagagt atattgctgt tatttccatg
    54121 cagggctaag ggtttatgat gtatcagacc catattatat caaagagcta gcatatttta
    54181 taccaccaaa tccaaataaa acaccagaag aatcttattt cccaggattc ccaggaccac
    54241 gcttggcagt aacagaagat cttatcgttg atgatagagg ctacatcatc atagatgctt
    54301 tagatgatgg attctatata ttaaaaatga aagaagatta aataaatact aaagagggag
    54361 agttgaaaat gaaaatacta ggtatttctt ttggaacaaa aaatggaaat aatgacacaa
    54421 tgtgtaaagt tgcacttaaa ggtgccaaag aagcaggggc tgaagtagaa tttattcaaa
    54481 tgtcatctat taacattaaa cattgtactg gatgttgtgc gtgcgttaaa actttattat
    54541 ccgggaaagg tagtatgtgt gtattaaaag atgattttga atggttgctt gacaaaatga
    54601 aagatgcaga tggtatagta gtttcagacc ctatttttga agaaggggca tcaggattat
    54661 tccatactat aatggaccgt tttggaccca gagcagatag aggaaataac attataggaa
    54721 caaaagttgc agaagctata ggaggaaaaa ttccagactc aagaatgctt aatgataaag
    54781 taatatcatt tatgggaata ggaggttctg actggggtac aagagttcaa tgtgaacatg
    54841 ctatgctagc tctcatacca atgtggaaag ttatagataa tgcttggttc ccatgggcaa
    54901 aggaattagt aatggatgat acaaggcttg ttcaagttca tcaaattggt ttaaatatag
    54961 ttgaagcagc aaaagatttt gaaaaagctt catatcaagg agaagaaggt gtatgtccac
    55021 attgccacaa tagattattc taccttgagt caggtactaa aaaggcaatt tgtgctctat
    55081 gtggtatagt tggagagtta gatactataa gtggaaaaac tatatttagt tttccagaag
    55141 agcaattagg tcatgctcat gatactttac caggtaagtt tatacatggt gatgatatta
    55201 agaaaatgga aggtcattat gcagaagttc gtaaaacaca agaatttaaa gaaagaaaaa
    55261 catcttataa ttttatagag ccattaacta aagaaaagca agctggagtt tcattaggat
    55321 aataattaaa atactaagac tcttttttaa gggatgttag atgttatagg aggcttattg
    55381 tatgaatgaa aagccaagtt ttaaacaaat tgaaattatt ttagcagtta tttttttggt
    55441 acttacatat tacaagattg gattaactcc agcatttatt gttatattta taccattata
    55501 cttaagttat tttgcagtat ctatgattgt aaatcaaata gcaattaagc tattggagat
    55561 aaggcaagct agggaagcta ctaattacaa tataggaaat ggatagaaag aaaaaagaaa
    55621 tattaacact gtctatgggg attgctttac tacctccttt atgggcagta ttagcacctt
    55681 atataggaat aaaaactgga gctgtagcac taatctgtgc aggattatat gtaactaatg
    55741 gtaataaaca aaaagatgga ttaaaaataa tgtttggttt ttggtgtgga gatttatggg
    55801 cagtgttagc cattttaatt atgggatata tgaatttcaa tcaaaaccta gaattatttt
    55861 taacacttag tatattaggt ttttttgcag ttgtaattgc atccttattt gaaaaaataa
    55921 ttttcctacc atcatggctt tgtggttggg ctataggtct aactattatg acaggtgaaa
    55981 atattattaa tttacaagat atttgtattc aaattgctgt agctatggca gtaggagtat
    56041 ggtacgtggg tgcaggtgta gatttgttta caatatatat tttgagtaaa gataagaaaa
    56101 agggaaattg aaagcatgtg aatatacttt gattatagga gaatggctat gggttttgaa
    56161 tttaaaataa tgagaagttt aatatatgta ggacttgcca aggaagaata tagacctaag
    56221 ttaatggact ggttatatcg tcaccatatt ccagatagta ttagcacttt tggaccatat
    56281 tgtactaaat atgcctttta tcaagcatat cctacaccaa atgaaggtga gcgttttggt
    56341 gcacgtaaga tgcaactaac agaacattat tggcttgtag atgaacatat gcctgagatg
    56401 gcaaatagaa ttatgacaga atatatgcct atggatgttc tacgttggca agggtgtata
    56461 ccagatgtag aaaataaaag ggttcatgaa aatgcagaaa gtggagatgc aggacgtgca
    56521 gtaggtggag ataatggatg cccaccattt atatttgctt ttgttccaat aaactgggaa
    56581 gaagacttta gaggaaaagg acgtactgta caagatggac caaactatcg ttggcaattt
    56641 atgattaagt atccagatgg tatctctaaa gaagaaggag aaaaatggtt ctatgatgag
    56701 gtagtgccat actttacaaa ctgttgctat gttaatcgtt ttgtcagtag taaaataatg
    56761 attaattatg gagcaactgc ttttgaccgt gtatcagaac tatggtttga aggggaagag
    56821 gaatggtata aagctgtggt tgaagagaca aagtcgttta ttaaaaaacc agaatgggca
    56881 caagaagagg agttcccata tttaaaacca caattcaata tcgcatcagt attcttaggt
    56941 gatatagcaa ctatggatgc atactcacag tatcgtggat atataccaat gagataaaaa
    57001 ttatagtttg tgataagtag aggaagtact tactcttact gctagaagaa gacttattgt
    57061 acacttaaaa gaatgaaata gataagtgtc aatttattat atgagagaaa ctttaacttt
    57121 cttggtttaa aatattaaag tcataaggaa catttattat taaatacttt atttatatga
    57181 attaattgaa gaaaaaggat attaaactac aagttaaaaa ataaatttaa tatattagaa
    57241 ttataaataa ggcacattaa ttttatttta aattaatgtg ccttttattt atggtaattt
    57301 atgcataatt agtatcgctt tgattcttta aataaatttt ttttggtttc tttacataag
    57361 gaaccctttt tta
//
LOCUS       DAEPOZ010000004        47702 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.004, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000004 DAEPOZ010000000
VERSION     DAEPOZ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 47702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47702
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.004"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..1350)
                     /locus_tag="KR440_000192"
     CDS             complement(<1..1350)
                     /locus_tag="KR440_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBH0697263.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     "
     gene            complement(1361..2581)
                     /locus_tag="KR440_000193"
     CDS             complement(1361..2581)
                     /locus_tag="KR440_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBH0697264.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(2595..3383)
                     /locus_tag="KR440_000194"
     CDS             complement(2595..3383)
                     /locus_tag="KR440_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBH0697265.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKDENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(3400..4674)
                     /gene="larA"
                     /locus_tag="KR440_000195"
     CDS             complement(3400..4674)
                     /gene="larA"
                     /locus_tag="KR440_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HBH0697266.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(4792..5505)
                     /locus_tag="KR440_000196"
     CDS             complement(4792..5505)
                     /locus_tag="KR440_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBH0697267.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            5630..6889
                     /locus_tag="KR440_000197"
     CDS             5630..6889
                     /locus_tag="KR440_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HBH0697268.1"
                     /translation="MKKNKIIAFFAPAIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKHEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFIKKFSRKK"
     gene            7157..7723
                     /locus_tag="KR440_000198"
     CDS             7157..7723
                     /locus_tag="KR440_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBH0697269.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKHLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(7924..9129)
                     /locus_tag="KR440_000199"
     CDS             complement(7924..9129)
                     /locus_tag="KR440_000199"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBH0697270.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     regulatory      complement(9232..9338)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(9430..9798)
                     /locus_tag="KR440_000200"
     CDS             complement(9430..9798)
                     /locus_tag="KR440_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HBH0697271.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(9850..11127)
                     /gene="obgE"
                     /locus_tag="KR440_000201"
     CDS             complement(9850..11127)
                     /gene="obgE"
                     /locus_tag="KR440_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBH0697272.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(11238..11528)
                     /gene="rpmA"
                     /locus_tag="KR440_000202"
     CDS             complement(11238..11528)
                     /gene="rpmA"
                     /locus_tag="KR440_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBH0697273.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(11542..11859)
                     /locus_tag="KR440_000203"
     CDS             complement(11542..11859)
                     /locus_tag="KR440_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBH0697274.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            complement(11874..12185)
                     /gene="rplU"
                     /locus_tag="KR440_000204"
     CDS             complement(11874..12185)
                     /gene="rplU"
                     /locus_tag="KR440_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBH0697275.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(12286..13707)
                     /locus_tag="KR440_000205"
     CDS             complement(12286..13707)
                     /locus_tag="KR440_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HBH0697276.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLIIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(13725..14426)
                     /locus_tag="KR440_000206"
     CDS             complement(13725..14426)
                     /locus_tag="KR440_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HBH0697277.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(14428..16272)
                     /locus_tag="KR440_000207"
     CDS             complement(14428..16272)
                     /locus_tag="KR440_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HBH0697278.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFI
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            16591..17784
                     /locus_tag="KR440_000208"
     CDS             16591..17784
                     /locus_tag="KR440_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HBH0697279.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            complement(17996..19078)
                     /locus_tag="KR440_000209"
     CDS             complement(17996..19078)
                     /locus_tag="KR440_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBH0697280.1"
                     /translation="MKKIRKLATKLLIATMVLTSCMSVTVYGMTAKDVTAKTPKSYVA
                     GTNSIYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKIRVAYNHIKNNTTYVNWNA
                     AEGANTAYTLVTKKGACSGMCRSLKALCDAMGIECYYVHSTTNDHQWNLIRFEDGRLY
                     HVDIDANKSAGADIIYKSLTHPLPFDKKAYPAVGESVKTQNPTPTVKKPTPAKPSAPV
                     GPGNHTMIADQEEAILDEVYGNNLRPKIERYEGVPCSLINSSINTIKFTSKNSNLDPS
                     RHTVSVIGFMNTPYGMVALAPDGVPVEYPFEYDKPFSLDFTGKNLSAAELSKKCKNNN
                     VIIKIEIGEIEPNSGRGQVRYARYVN"
     gene            complement(19642..20433)
                     /locus_tag="KR440_000210"
     CDS             complement(19642..20433)
                     /locus_tag="KR440_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697281.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTNIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFAFGLLCIIAYFILRFKISLIKNNN"
     gene            complement(20437..20766)
                     /locus_tag="KR440_000211"
     CDS             complement(20437..20766)
                     /locus_tag="KR440_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0697282.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
     gene            complement(21251..21664)
                     /locus_tag="KR440_000212"
     CDS             complement(21251..21664)
                     /locus_tag="KR440_000212"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBH0697283.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
     gene            complement(21705..22835)
                     /gene="rodA"
                     /locus_tag="KR440_000213"
     CDS             complement(21705..22835)
                     /gene="rodA"
                     /locus_tag="KR440_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HBH0697284.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            complement(22920..23204)
                     /gene="minE"
                     /locus_tag="KR440_000214"
     CDS             complement(22920..23204)
                     /gene="minE"
                     /locus_tag="KR440_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBH0697285.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            complement(23222..24019)
                     /gene="minD"
                     /locus_tag="KR440_000215"
     CDS             complement(23222..24019)
                     /gene="minD"
                     /locus_tag="KR440_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBH0697286.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            complement(24043..24726)
                     /locus_tag="KR440_000216"
     CDS             complement(24043..24726)
                     /locus_tag="KR440_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBH0697287.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYENESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            complement(24824..27802)
                     /locus_tag="KR440_000217"
     CDS             complement(24824..27802)
                     /locus_tag="KR440_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBH0697288.1"
                     /translation="MEENKQVDRLKIIKIVMSVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMLTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKMNVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            complement(27814..28302)
                     /gene="mreD"
                     /locus_tag="KR440_000218"
     CDS             complement(27814..28302)
                     /gene="mreD"
                     /locus_tag="KR440_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBH0697289.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            complement(28317..29210)
                     /gene="mreC"
                     /locus_tag="KR440_000219"
     CDS             complement(28317..29210)
                     /gene="mreC"
                     /locus_tag="KR440_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBH0697290.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKDMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            complement(29220..30230)
                     /locus_tag="KR440_000220"
     CDS             complement(29220..30230)
                     /locus_tag="KR440_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBH0697291.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     INMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            complement(30351..31010)
                     /gene="radC"
                     /locus_tag="KR440_000221"
     CDS             complement(30351..31010)
                     /gene="radC"
                     /locus_tag="KR440_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBH0697292.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            complement(31055..31639)
                     /gene="maf"
                     /locus_tag="KR440_000222"
     CDS             complement(31055..31639)
                     /gene="maf"
                     /locus_tag="KR440_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBH0697293.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            complement(31859..32830)
                     /locus_tag="KR440_000223"
     CDS             complement(31859..32830)
                     /locus_tag="KR440_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HBH0697294.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            complement(32846..33421)
                     /gene="rsxA"
                     /locus_tag="KR440_000224"
     CDS             complement(32846..33421)
                     /gene="rsxA"
                     /locus_tag="KR440_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBH0697295.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            complement(33436..34029)
                     /locus_tag="KR440_000225"
     CDS             complement(33436..34029)
                     /locus_tag="KR440_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HBH0697296.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            complement(34042..34611)
                     /locus_tag="KR440_000226"
     CDS             complement(34042..34611)
                     /locus_tag="KR440_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HBH0697297.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQENNEL
                     RKAVLPEAKEFKEMDKSTYKDLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            complement(34611..35588)
                     /locus_tag="KR440_000227"
     CDS             complement(34611..35588)
                     /locus_tag="KR440_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HBH0697298.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            complement(35611..36948)
                     /gene="rsxC"
                     /locus_tag="KR440_000228"
     CDS             complement(35611..36948)
                     /gene="rsxC"
                     /locus_tag="KR440_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBH0697299.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            complement(37164..38108)
                     /locus_tag="KR440_000229"
     CDS             complement(37164..38108)
                     /locus_tag="KR440_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697300.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            complement(38219..39493)
                     /locus_tag="KR440_000230"
     CDS             complement(38219..39493)
                     /locus_tag="KR440_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBH0697301.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSNQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKSKNNGWYKVKLSSGTIGWVSASYISETNEGTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            complement(39625..39996)
                     /locus_tag="KR440_000231"
     CDS             complement(39625..39996)
                     /locus_tag="KR440_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH0697302.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            complement(40224..40823)
                     /locus_tag="KR440_000232"
     CDS             complement(40224..40823)
                     /locus_tag="KR440_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697303.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            complement(41024..41236)
                     /locus_tag="KR440_000233"
     CDS             complement(41024..41236)
                     /locus_tag="KR440_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBH0697304.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDISIEKLKEVIAELGFELKGIE"
     gene            complement(41309..43024)
                     /locus_tag="KR440_000234"
     CDS             complement(41309..43024)
                     /locus_tag="KR440_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0697305.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEEARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANINSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEE
                     DRIKKEAEKKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            complement(43024..43578)
                     /locus_tag="KR440_000235"
     CDS             complement(43024..43578)
                     /locus_tag="KR440_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBH0697306.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            complement(43571..44173)
                     /locus_tag="KR440_000236"
     CDS             complement(43571..44173)
                     /locus_tag="KR440_000236"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBH0697307.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNISIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNSLTHPRIREKIISEIEELR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSKQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            complement(44182..46830)
                     /gene="polA"
                     /locus_tag="KR440_000237"
     CDS             complement(44182..46830)
                     /gene="polA"
                     /locus_tag="KR440_000237"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBH0697308.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKEYKAGRKGMPDELAEQLQPLKDLLDKFKINR
                     LEIDGYEADDIIGTVSKKAENNGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPAQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMVFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDTEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETIPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            47149..47231
                     /locus_tag="KR440_000238"
     tRNA            47149..47231
                     /locus_tag="KR440_000238"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47183..47185,aa:Leu,seq:caa)
BASE COUNT        14646 a         8961 c         5287 g        18808 t
ORIGIN      
        1 agctaacttt atatttttaa ttaacatcat ataagcatct gaatttgatt catgtaagta
       61 ctctttttta gcatatccta tgccatgttc accagaaact tgaccactaa gttctctaga
      121 ttttttatac atatagtcaa aagtttcttt taatcttatt ttccatgtat tatcatccat
      181 accatctttt aaaatatata tgtgtagatt tccatctcct gcatgaccaa aactttttat
      241 tcttattttt aatttgtctt gaagctcgtg agtgtatctt ataaactctg ctatcttatc
      301 tcttggaaca acgacatcac attcatccat ttgtgtcgta gatgccttta tagcttctaa
      361 aaatgcacct ctggctgacc atatagaatc gtttctttct tgtgtatctg atataaatac
      421 atctaatgca ccattttcta aacataaatt agctactttt tcatattcct tttctatatc
      481 ttcagtgcta tttccatcaa atgtaagtaa aagataagca tctgatgtat tatctggaaa
      541 ctttttacct aaaaattctt ctgctgccaa tattacatct ctttccataa attctatagc
      601 cgttggtatt gattttgatt ttattatttt aggaacagtt tctattgcca ttgataaatc
      661 tggaaatggt ataagcaaac ttatactttt ctttggtaaa ggaagcaatt ttaatatagc
      721 tttagtaact attcctaaag taccttctga acctactaat aaatccttta tactatatcc
      781 agaactattt ttaaccacct ttccacctac atttataatt tctccatttg gtagtacaac
      841 ttcaagtcct cttacataat cccttgtaac gccatatttt actgctctca taccacctgc
      901 attagtgttt atattacctg caattgtagc acttttttca cctgggtcag gtgggtaaaa
      961 taaatctcta tcttgaacat attgacctat tgtcatcaat aaaacacctg gttctactgt
     1021 caatgttaaa ttttcataat caacttcaag tattttattc attttacata gattaatcat
     1081 tattccacca tttatggcaa cagcagcacc tacaagacca gtaccttgac ctcttggtgt
     1141 gactggtata ttatttttat atgcgtattt cattatttta gatacttgtt cagtttcaag
     1201 tacatttact aaaacttcag ggtacttctc aatccctcca agttcatcat gtgaaaaatc
     1261 ttcatttata tcacttccaa ctaatacatt ttcttctcca caagtatcta ttaaaaaatc
     1321 tatatctttt ttatcgatta atttatacat ttctatcccc ctatttagct gctgatgcca
     1381 tatatttttg attattatct ttgttatttt ctattttttc aatcaattta ggaataatat
     1441 cataaatatc acctattaaa gctaaatgtg ctacatcaag tataggcgct ttatcatctt
     1501 tatttattgc tactatatag tcagcaccct gcatccctgc aacaaattga actgctccag
     1561 atacaccaca agttattatc aatttaggtt ttacagttct accactaaga cctatttgtt
     1621 ttttagcatc tatccaacct gcttctatta caggtcttgt tcctgccact tgagcgttta
     1681 gcttatcagc taatttatat atcatctcca tatcttcttg tttttttatt gctctgcttg
     1741 ccacaactat cacttcagct tcttccagac caacttcttt atttttttct tttacactca
     1801 gtaccttaat tttagatttt agttcttttt tttctacttt acataaagtt atctttcctg
     1861 tagtattctc tattttttct ggtgctgaaa atattttata tcttacagtg gcaaattgtg
     1921 gtctattatt aggtgtgttt atatgagcca ttatatttcc accaaatgct ggtcttattt
     1981 ggtctaaatc agtatttgat tgaatatcaa gtattgtaca atctgctgta agacctgttc
     2041 taaatctagc ggcaagtctt ggagctaatg acctacctaa tgtagttcca ccaactaata
     2101 ctatcgttgg ttttatttta tctataaagt tttctattgc ttttgaatat ggttctatcc
     2161 taaaatcttt aaattcatct tcatcatata caaatacttc atccacccca taagaaatca
     2221 aattactgca cttatctttt atgtcttttc ctacaaatac acaatacaca ggttgtttta
     2281 tttttgatgc catctcacga gctttaccta taagctcgta cgttacagga tgtatatttc
     2341 cattatgatg ttcaacatat actgcaattc ccctccattc atctttatta atttgtactt
     2401 tactactctc tataaattca aatgcgcctt ttggtccact tttgacacac aacttgcaca
     2461 tcttacagcc agcacttatt tctactatac ctgacttttc ttctatagcc ccaaaaggac
     2521 atatttctaa aattctcttt aagtcagtta ttttatcttg atttacaact atctttgaca
     2581 tttattactc ccccttacac caactttaat tcctctaatt tatttacaag tgcaaaactt
     2641 agttcatctg catttcctct tacaatattt ttatctgtat ttggctttgg aggaaatatt
     2701 cttacaactt gagtaggaga accgttaagt ccatagttat tttcatcttt atcttcaaag
     2761 tcatctaagc tatatacagg tatttctcta tctttagtat taagttttaa tttaaatgaa
     2821 ggtagtcttg gttgaaaaat gtctttttct acagttatta aacatggata ttcaatctca
     2881 catacttgta aatcatttgg catatctatt tcaacaacta tactttcatc tttaacttca
     2941 attaattttg taacgttagt aacatgaggt atatctaaaa attcagctac ttcaggtccc
     3001 acctgtgctg tatcaccatc agtagtttgt tttccacaaa ttattaggtc tacctttccc
     3061 atcttcttaa taccttgtga tatggtatag gaagtagcca atacatctgc tcctccaaat
     3121 ctcctatcac ttattaatgc tccttcatca gctcccatca taaaactttc ttcaattacc
     3181 ttctttgcct gaggaggacc catgctaagt acttttagat tcgctttctt atcttccttt
     3241 attctaagag ctgtttctaa agcatatagg tcatagggat tcatttttga atctactcca
     3301 tctcttttta gtactcctgt agtttcatca acttctactg aagtcgttcc aggaacttgt
     3361 ttaatacatg tcaaaatttc cataaagtac actccttttt tatttaacta taactgatac
     3421 tccatcaggt ataactacta ctttgctctc cttaccaact atactattcg ccatttctaa
     3481 agcttcttta aagttaaatg catgtttcat atgcattttt tttatcattt caggattaca
     3541 cttatctgtt accattatca cagtatattt agataatatt ctcgctagta tctgaaattc
     3601 ccattggtct ggaatcgtat ctactctatc gacacttcta attttatcta aaagttgttt
     3661 tggagaatct gcatttgcta cattttcata aaaagattga cctccatgac catcattaca
     3721 tgctgaaatc attattatta ctccaccgtc tttacaagta gcttcagctg ctgtcattcc
     3781 tttaacagat tgataaatat tttggtctag tggatatcca ccatttgtag ttacaacaat
     3841 atcagatttt atagaattta cttttgataa ctctgttaca aatctacatc ctttagtatg
     3901 tgctttttcg acatcaccag caaaggaagc aattattttt ttatctttat ctattacaac
     3961 attcaatatg aatgctaact ttgcaacttt agctgcatat accatatcct catgaatagg
     4021 attattattc aatttcccag ttcttgaatt atcactatct ataaagtcac tacagtgatt
     4081 gtacattata gtctttgcag atgcgattcc tggaagtacg ctttttctgc cacctgaaaa
     4141 acctgcaaag aaatgaggtt ctataaaacc ttctgctatc aaaagttctg cctcatatgc
     4201 tagtttgttt atatataaat ctccacctga aggcaatatc cctactttac acatagattt
     4261 atcatcagta gatatatgca taactatatc ttcattatct actatttctt ctcccatttt
     4321 ataaattagc tcttcccttg tacttggcct atgaaatcca gttgccacta taatcttcac
     4381 atcaatttct ggattaacat tcctaatcct tcttaatata attggcatag ttattctact
     4441 tggtacaggt cttgtatggt cacttgtaat tatgactaca ttctttttcc ccttaaccag
     4501 ttcttccaga gaattagagc ctataggatt attcatagat tcttcaacta tctcttcttg
     4561 agtcttatgt tctatataat tatccgattt tgactccaaa acaccgagaa aacgttcatc
     4621 aggtatttct aaaatcattc cttgttttga atacggtact tttaacttta ccatttttaa
     4681 tctcccatta taataaacta taaaattggt atgtggtcat accagtttca catttatatg
     4741 atagtaccat tatctaccat ttgcaagaat aatttatttt ataacaaact tttaattttc
     4801 attttttcta taatttttac ttataaaatc catatgttcc tttgctttct cacatgcaag
     4861 ttctacatca ttttctacaa ctgcattgta tatagcttca tgctgtttaa gcaatatatt
     4921 ttcattaaaa ggttctgcga ttattttttc tcttgcatct tctataaatc catcaaataa
     4981 ttttgatgct gtcataaaaa gactttgaat taaataattt ttacttaatg ttgctagttt
     5041 actgtgaaat ttttggtcta ataacaccag tactcgatta ttatcttttt tattagtttc
     5101 tactctcatt tcatctatta tatgtttcaa ttctataact tcagaagaag ttattctctt
     5161 tgaagcaagt tttgcaatct caatttctag gattattcta agctctaata tatcttcaag
     5221 tgtgccatta ttaagtttaa aaatcataga taaaggttct attaaagttt tttctatatt
     5281 tgtacatata aaatttcctt caccctgtct gctttctatc actcccatag tctctaatac
     5341 cctaatagct tcccttatgg aagttctact cacattcatt tgttcagaca attctctttc
     5401 tgatagtaat ttatccccct tttttaactc tccatttagt attttatatt gtatttgctc
     5461 aattacttgt tcataaactt ttttactagc tatactatta aacatttttt cctccaatat
     5521 gataatcgtt gttgtaatat atatctatta tatatcttaa accattacat tcatatattt
     5581 ttattacttt taatataata tcttataact caaatttaca ggtgatatta tgaaaaaaaa
     5641 taaaattatt gctttttttg ctccagctat tatagttgga gttctagttt tagggattat
     5701 cctataccca aatgaagcta taaaatctgc aagagaaggc ttctctatat ggtctaatgt
     5761 tttagttcca tcactactac catttataat tggagcaaac ttaattgttg atttaaaaat
     5821 agtggatatt atagggttta tcatcaatcc cataactaga tttgtattta atgtttcagg
     5881 aaagagcgct ttagcttttg ctatatctac agtatctggt tatccagtag gagtaagttt
     5941 agcttctgaa tttaggagta atgggcaaat ttctaagcat gaagcacaaa gacttgtgtc
     6001 tttttgttct acatcaggac ctttattcat aataggttcg gttggtacag gtatgttcca
     6061 aaattcaaac ttaggttatt taatgattct ctgccattat ctaggaacta tattggttgg
     6121 tcttttcttt agaaattatg gtaatgaaaa tcttccaaag acaaaattgg atttaaaaac
     6181 aaatataaaa aatgtaatca acattagaaa ttcaagtggg aatggttttt ttgttttatt
     6241 tggaaatgct gtatttaatg gagtaaatac attacttaca gttggtggat ttgtaattgt
     6301 tttttcagta gtatttaaaa tactatctct ttttaatgta atctcattaa tagcatcagt
     6361 tatatatttg cctctttcat ttttaggtgt tagtaaagaa ctctgtcaag catttgtaag
     6421 tggtctattt gaaattacta taggatgcaa taaagtatct agtataacaa gttctccaga
     6481 aattttaaga gcatctttag ctagcttttt gataggattt agtggattat ccatcctagc
     6541 tcaatgttgt acatttctat caaaaacgga catagattta aaattataca tactaagtaa
     6601 atttgcgcat ggagttttag ctgctatctt tatatttata ctttatccaa ttgcaaactc
     6661 tgctgtgttg gtttctagct ttgctgatac atacaacgtt atatataata atttaatctg
     6721 gttttattat cttagctatt atgatacaat tcttcaaatt gtagtaataa tttatctaat
     6781 ctcagcagta tttattgcaa aaaaacatat gaaaacttta tctacaaatg ggaaactaat
     6841 gcgatataaa aatacttttt ttattaagaa attttcacga aaaaaataaa actaataaaa
     6901 cttaactaaa tacttccatt tttttattat atttgtcata attttaataa cctttttaat
     6961 tagtttaaca gcatagttat ttcatcacaa tttaataatt ttattagaaa tatttttcaa
     7021 aaaattctcc ttaatcacat atatcacact tgtataagtt tactgggaaa tttttccatt
     7081 atataatgtt tcatttatat tatataataa ataccgacct aaacttacca aatcaaacta
     7141 agaggagtgg ttgaaaatgg caacaagacc tatagaaata gacgaatata aaaagataat
     7201 ggaactatta catactggct ttacttatag tgaaaatggc gttgaaaaaa gatttagaaa
     7261 aaatcctaaa gttgcattgg cactaatgtt agaagctaat ttaggtctaa gaatatcaga
     7321 tattttaaga ttaaagatag gtaattttaa aggtaatatg ttggaaataa atgaaaaaaa
     7381 gacgggcaaa ttacagtata gaccagttaa taaaaatata attgagtcca taaatgaaca
     7441 tgcaagaaag tataatctta aatcaaatga ctacttagta aatataaaga caaaggctat
     7501 tcaaaaacag ttgaggataa tttgtaaaca cttaaatctg tataatataa gtacacatag
     7561 ctttagaaag ctctatgcta ctactcaatt tgaaaagagt aacaataatt tagagttggt
     7621 taaagaactt ttaaatcaca gttctgtagc cactacacag cgatatataa gagtaactca
     7681 gcaagctatt aatgaagcta gtgaaaattt ctttattggt taattataac gtatacattt
     7741 ttctattatc ttattaattc accagatatt aaaaaagagt gtctcaaaat gaacttttta
     7801 gttcatgaga cactcttttt ttataaaatc aaatatattt ttattatttc aatactggaa
     7861 aaaaggcttg tctctctatt ttgagacacc ctattaaatt ttcataattc ttgcataaac
     7921 atattaatct atcttcaata attttttagc tgattcactg taattatttc tttttacaat
     7981 ttccaaaact ctatcaatct catgttctga ctttgcactt acaaaaatcg ccaattcttc
     8041 atcatctata atatcataac tatctacata tttatctaaa cattctaaaa ttccatcttt
     8101 attattatac ccaccaaatc taactactgc actacactca tcttctataa gtccacttaa
     8161 atccaatttc ccttttgaaa aagactttat atttgaactt ctattatcta ttatatctat
     8221 aatatctgca taaacagcac ttcctgttgc acctcttcca gctcccttac ctacaaaaga
     8281 aagttcacct actgaatcac ctactaaaat aactacgttg aactcattat ctatctttga
     8341 aagcatatta ttattgtcca ctaatgctgg tctaacaaat ccacttactt ctcctgattc
     8401 atacttactt ctagcaacca attttatctt acaatttaat ttttttgcat attctatatc
     8461 tttttcatct attgaagtta ttccttctaa taataaatct ttccaatata ctatttttcc
     8521 ataagataaa gtagacaata tagataactt tctagcacta tcatatccta aaacatctgc
     8581 atctggatta acttctgcaa atcccaactc ttgtgcctgt tttaaagcaa catcatatgg
     8641 taaattttca tcatacatct ttgataatat aaaattacaa gtcccattta aaatagcgtt
     8701 tatgctcttt atattgttgc cttccagtga ttctatcaat ggttttaaaa ctggaatacc
     8761 acctgccaca gatgcttcaa acttgatgct aacattattt aagttagcta attctataag
     8821 gtcactacct ttttcagcca gtaaatcttt attagctgtc actacatgta tttttttatt
     8881 taacgctctt gttacataat caaaagctgg atttaatcct ccaattactt caactagtat
     8941 atcaatatcc ttgtcaaata tatcttctat atcagtagtt atcttatcaa aatacttctt
     9001 atacttgtgt ttttctttat ttttaactaa aattccaaca acttctatat ctctttttga
     9061 agtattattt tctattaaat caattaatcc acttcctact gttccatacc ctaatattgc
     9121 tatcttcact gtaacatacc cccatcattt tgattatatt aaattattta tatatataaa
     9181 tataccaata aaaaaagctt gagtataaac tcaagccata tgacgggttt atactcatct
     9241 ttcagcataa atgctgcagg atttagcacc aatgtataat taaatatact ggttgccggg
     9301 cttcacaggg ccagtccctc tgccactctt gataagattg ctaattaaaa tttatgttta
     9361 atagtataat acaatttctt ccaattgtca ctattttttt aacttatttc cctaaaagga
     9421 tataaactat tatctaacag ctcttttagt tggtttaccc ttttttgtaa cattattatt
     9481 tttagtcttt gctttcgcct gttcatgtcc tggaaaaagt attttaggct cttcaatatt
     9541 cctcttgtaa atagtaaatt taaaaccaat agcctgcaca aattcagctc ttaaagcttc
     9601 acatactgca ttagcagttt ctttagtatc aagacctgca ttttctaata tagtaacttt
     9661 tactaattct ctagttctta acataccatc taattgctct aagaaactct ctgtaacacc
     9721 ttctttacca atttgtgtag taggctttaa agtatttgct aaagatctta aatacgccct
     9781 ttgttttcct tttaacatat tctcacctct ataattctat acttaaatta tctataatct
     9841 atatttaaat taattgtaaa actcaaattc taattcataa attttaacca catctccatc
     9901 ttctattccc atttctctaa gcctatcaaa aacaccttga ctctccatag ctttttggaa
     9961 atattgaagg gattccatat catcaaaatt tacagaatac attattcttc taagtgcttt
    10021 acctgtaact acatacacac catcttcaat atctatactt aactcttctt cagtaccaat
    10081 atcaagttct ggtctataca tttcttcttc tgaaactagc tccacatctt ctacttcttt
    10141 tagcatttta gaaacatagg ctattacttc atctatgcct tgacgagttg ctgctgacat
    10201 tttaaatact gtatatcctc tgccttctaa ttcactctta aacttctcaa acttactctc
    10261 atcttctaat atatcaaatt tatttgcaac tacaacttgt ggcctctttg aaagcttttc
    10321 attgtacaat ttaagctcat catttatctt gtcaaaatct tcaatagggt ctcttccctc
    10381 taatccagat atatctacaa tatgtatcaa tacctttgtt ctctcaacat gtcttaagaa
    10441 ctcatgacct aatcctatgc cttcagaagc accctctata attccaggta tatctgcaag
    10501 tacaaaacta tctccaaact tagtttgaac aacacctaga tttggtgtaa gtgttgtaaa
    10561 atgatagttt gcaattttgg gtttagcttt agttacaact gatagaaatg ttgatttacc
    10621 tacatttgga aatcctaaaa gaccaacatc tgctatcatt ttaagttcaa gagttatcca
    10681 tctttcttct ccatctgtac cagattttgc aaaagctgga gcttgtctta ctgcatttgc
    10741 aaagtgttgg ttacctttac cacctatacc accttttgca actatagctg tatctccttc
    10801 tttttttaaa tctgctaata caagacctgt agcctcatct cttattactg tacctgctgg
    10861 tacttttaat actaaatttt ctccattttt accagctctt tttcctttgc ttccgtcgcc
    10921 accattttga gcttgatatt ttttctggta cttaaagtcc attagagttc taagccccaa
    10981 atcaacttca aatatgatgc ttgctccacg accaccatct ccaccgtctg gaccaccagc
    11041 tggaacgtat ttttctcttc taaatgctac cgaaccattt ccaccatttc cagccttaac
    11101 aaatattctt gccttatcta taaacaaatt cttcacctac ttaattaaaa acttttaatt
    11161 agcattacca aaaataccat tataaattca tcagtattat aagaaaaagg agtgcctcaa
    11221 cactcctttt atgtatatta ctcagctatt gctactggat atatactaac tttttttctc
    11281 ttcttgtctt ttctttcgaa tttaacagta ccatcaacta atgcaaataa agtatcatca
    11341 ctaccttttc caacattagt tcctggatgt attttagttc ctctttgtct aactattata
    11401 cttccagcag ttactaattg accatcgaat ctcttaacac ctaatctttt agatatagaa
    11461 tctctaccat ttttagaaga acctacccct tttttagatg ctaataattg taaattcatg
    11521 tttaacatat ggagtcaccc cctacttttt taaaattttt atattttttg ggtattcccg
    11581 tttaatttct ttaaccgcta attgaaagtg atttagtaat aattgtgcgt cttttacatg
    11641 cgtatctttg actatacact ctatatgacc ttcttctcct actacttttt caaattcaat
    11701 cttttgtaat ttgtctaaag aatttataac tgcaatagca ttagatgtaa ctgcagcaca
    11761 tactatatca tagcctattt cagcaaaatc agcatgtccc tttaaacaaa aaccttgcaa
    11821 caagaaatca tcattataat aatatttaac ttttatcata acttaaccaa taactaagcg
    11881 tttatttttt ctacaactat cttagtgtat gattgacgat gtccttgttt tcttctgtaa
    11941 tctttttttg ctttatactt gaaaactata actttcttag ctttaccttg ttcaacaact
    12001 ttagcttgaa cagatgctcc ttctactact ggagaaccta attttaattc tccatcttta
    12061 gagcaagcta atacttcgtt taaagtaaca acatcacctg cgttagcttc taatttttca
    12121 acgaataata catcaccttc agaaacttta tattgctttc caccagtctt tactatagcg
    12181 tacattaaat acacctcctc ggcctcaaac tcgccagtac aaggtacttc atttgaagat
    12241 ttaaaccttt actgtgcggc attacaatta ttaattataa aatatttaca ttttattgtc
    12301 aactatttta tcgaaaataa cattggtttt attgtgcttt aatgtactat ttgcgtttaa
    12361 agaaatcttt atattatatt tttcaccaat tttatcaatt atatccatat aatattcttt
    12421 tatttgttca aaaataaaat cattaaattc taccattatg tttttgtaaa cagtatgttc
    12481 acttattctc ataatttctt tttcaatact atctagtata taatgtacag attttattgt
    12541 ttgttcacca tcacaacatg gacaagacat taaataatac ttatcaatgg attctttttc
    12601 tctacgccta gcaacttcta ctaaaccaag ttttgtcata cctaatactt cagcttttct
    12661 tttatcttta ttaaattctt cttctaggat gtgtaaaatc tccttttgat gcttcttttt
    12721 atgcatatca ataaaatcga ttattattat cccagctatg tctctaatta taagctgtct
    12781 tacaatttct tttgcagctt caagattagt tttataaacg gtttcatcaa gtttaccagt
    12841 tccaataaac ttacccgtat ttacatctat aaccgtaaga gcttctgttt tttcaattat
    12901 taaataacct ccactcttta accatacttt tttatttaat aatttttcta ttttagattc
    12961 tattctgtat aaatcaaata catctctatt ttcctcaaga cacagtttat caacataagt
    13021 cttatctata cctcttaaaa tagacttaag ttcagagtac ttatcataac tattggtaat
    13081 tatagattca atatcatcat tcacattatc ttttacatat ttagttgcaa aatctaaaga
    13141 cttatataat aatttcggac ctataccaag tttatattct tttaaaatat tttcatactt
    13201 taatttcaac tcttctatat ctttctttat ttcatcatga gtgcaacctt gagcctcagt
    13261 ccttattatt aatcctagat tttccttatt tactgcctta gtaatttttt ttaatctaaa
    13321 tcgttctttt tcatcagtaa ttttatttga tattgtagtt ctatcattgg aaggtatata
    13381 gacaatatac cttccagaaa tactaatctc agtgtttaat tttgcgcctt tagttcctat
    13441 ttcttcttta tttatctgaa ccaaaatatc ctgaccagat tttatagtac ttcccttttt
    13501 taattgtaaa taagcaagtt tcttaaatcc aatatctaca aaacaagctt ctattcctgg
    13561 tataactttt tttacgattc cacgatatat attggataca gtcttcttat ttaaattgtc
    13621 ttcaacaaaa agttctgtta atctttcatc ttcaagtact gctgttttct gggaccctat
    13681 tagggactca ataactatct ttttcacaac ttcacctctc gagcttacat aggagtcact
    13741 aattccccat cttctaatac atataaatct cttctcaata tatcaacatc taatgggtca
    13801 atatcaagtt caaacatctc taatatcttt ggtataagta tattagtatt taaattagtt
    13861 tttgagcctg tagctatagt agcagtaaga gtaatatgtt catcttctaa atttaaaaca
    13921 tcaaaggttc ttatcatagg tcttatatct acttcaacca tcttaccttt tttattcttt
    13981 tttgttatga ttatttcttt attattcata aagtcaatta cttttctttt tacaaactct
    14041 ttcgtcatag gtcttttcaa atctatatta aacaaatatt ctccatagtc tatcacagaa
    14101 gaaagagaag gtgtttgtct atcaatttct tttgctttga taaaatctat cccatctgga
    14161 agttgaattg atactttatt taaaaattcc tctactgata aatcatcttc ttctatttca
    14221 atatctacat actcaccttg actttctgtc cctaaagcca aagcattgcc ataactcatt
    14281 ttagggtgag gattaaatcc ttgagaatgt gataagttta tttcagctct tctaaacgct
    14341 ctttgtaaca atctctgaag gtcaagatgt gatatatata tcatgtcgcc ttccttttta
    14401 aattttactc ttattatctt actcatatta gcaaagcccc cttcctatat catgtatatt
    14461 tattccacaa ccattgcagt tatgtctaca atcagaagtg attttttctt ttttagcatt
    14521 ttcgtcttct ctaattaaga attttttact aatacctaca tctatatggt cccaaggaaa
    14581 tacctcttca tagtttctat ttctatgtgc ataaaaatct atagaaagat tattcttttc
    14641 catagcttct ttccatatat ctaaattaaa atgttctgcc catccatcaa atttagcacc
    14701 tagtctaaat gcatcatata taacttttcc tattcttcta tcgcctctag ctactactgc
    14761 ttccatcaaa ctagtctttg aatcatgata attgtatttt atattgttat ttcttaattt
    14821 atttactaaa tgtctctgct tattaataac ttcttctgta gtatcttgac catgccattg
    14881 gaaaggtgta aatggctttg gtacaaatgt agaagtactc actgtaacac taaaacttct
    14941 ctttagcttt cctccattta cttttctata aatatcaata actttataag ctaatttagc
    15001 tataccatct aaatcttcat aagtctcagt tggtaaacca atcataaaat acaacttaac
    15061 actattccac cccatctcaa aagctctttc tgttgcattt tccaaatctt cttctgtaac
    15121 acctttattt ataacatctc taagtctttg agttcctgct tctggagcaa aagtaagtcc
    15181 agattttctt acttgttgta ttttatcagc aatttccatt gaaaaattat ctaatctaag
    15241 ggatggaagc gaaatcccaa tattattaga tgcatattca tctaccaaat aatcagtcaa
    15301 ctctgataac ttactataat cactcgtact aagtgatgat aatgatattt catcatatcc
    15361 agtattctta actaatttat ctaatatttc tttaagtctt ttaacactct tttctcttat
    15421 tggcctgtat atcatacctg cttgacagaa tctacatcct ctagtacatc ctctaaataa
    15481 ttctagtact attctatcat gaacagtatc tatataaggt actattagtt tttctggata
    15541 atctacagtc tcaacatctt ttattatcct tttagtagga tttttagtaa tcccttctct
    15601 attaggagtt acactttgaa ctgtaccatc ttcattgtac ttgacatcat aaaaacttgg
    15661 tatgtatact ccttctatgc tagatatttt atataaaaaa tcttctctag tcgttttatt
    15721 tttcttccat tctttatact catttactac ttctaaatta acctcttcac cttcacctaa
    15781 tattactaca tctataaaat ctgctaatgg ttcggaatta taagcacaag gaccaccaac
    15841 cattataaat gggtcttcca aagttctttc ttcttttaat attggtatat tggctaaatc
    15901 taatatattt aagatattag tgtatgaaag ttcatattga agtgtaaaag tgacaaaatc
    15961 aaagtttgtt ataggctctc ttgactctaa tgcaaataat ggtatacttt tttctctcat
    16021 tatattttcc atatcaactg caggagaata cactctctca caaaatacat cttcatcctt
    16081 atttattaca tcatataata tatgactccc taagtgactc ataccaactt catataaatc
    16141 tggaaagcaa tgtgcatatc ttatcaattc actattacta gtatctttat gaattgaatt
    16201 tatttcattt ccaagatatc tagcaggctt ttctactttt tttaagattt ttttcaagtc
    16261 tactttattc atttattaaa attcctccaa acaagctctt tatttttatt cttatttata
    16321 agtagtatta tacacgataa aatattttct atcaaaaata taactaaaat attgaattta
    16381 aaatacatat tattcacaaa atagaatagc taaaaatatt taagaatatt ttctattaaa
    16441 tttttattga aatacaattt ttttacaaag tatgatatat tataaacata aaactattgt
    16501 tttacatgac ttatcatgac atcttttgtt tcattcacct caaaattggg tatgttactt
    16561 atataatgat aaattttagg agggttaaaa atgagtaaag tttttgaagt aaaaaaagat
    16621 atctacttta ctggcgtagt agatgaaggt ttaaaagtat tcgacataat aatggaaact
    16681 gagtttggga caacttacaa ctcttattta atcaaggacg aaaaaacggt tttatttgac
    16741 actgtaaagg caaattttaa agatgagttt ttaagtaatt tatcagaggt tactgatata
    16801 gctaaaattg actatgttgt aattcatcac acagaaccag accatgcagg tagtttaaaa
    16861 tacttattag atataaatcc taatatagaa gtttattgta ctaaagctgc taaactatac
    16921 ttagatggtc aaataaatag accttttaac tgtcatgtaa taaaagatgg tgaaatttta
    16981 aatatcggta aaagaaatct tagatttata actgctccat tcttacattg ggttgataca
    17041 atgtttactt acattgagga ggacaagact cttttaactt gtgatgcatt tggttgtcac
    17101 tttgcaagtg ttgatgcaga ggtagtgaat agtgaagatt atctaaaatc agctaaacac
    17161 tactatgatt gtatagttaa gccatttgct aaacatgttt taagtgctgt agataaggtg
    17221 gtaggactaa acatagaatt tgatactata ttgacttctc atggtccaat gcttactaaa
    17281 gacccaatgg ctgctgttaa aagatatgtt gagtggtcta cagaagctgt taatacaact
    17341 aatcaaaatc aagtttctat attctactta tcagcttata gtaatacttt agagatggct
    17401 aaaaagataa aagaaggact tgataaagaa ggtgcaaagg ctgaattata tgatttagaa
    17461 gatatgacat taacagaaat gcacgataca ttagtagtgt caaaagtaat attactaggt
    17521 tctccaacta taaacaaaac tatggttaaa cctatgtggg atttattctc tgttatagac
    17581 ccaatggcta accaaggcaa gatagcagga gtatttggtt catttggttg gagtggtgaa
    17641 ggtataacta tggctgagac tttacttaaa tctatgtcat tcaaaatgcc tgtagaatct
    17701 cttaagaaaa aattcttccc tagtgaagag acattaaaag agtgtatggc atttggtgca
    17761 gaatttgcaa aattagttaa atagccatta atcacacata atattattta aaataaatga
    17821 ttttatattt aaaaataaat taaaatggag ttgtctcaaa attaattttt ctaatctaat
    17881 tttaagttca actccttttt tatttcccaa aatgactaat ataatgataa atgcaccctc
    17941 accaagaggg tacattattt ataatctttt ctatttgtat acttgtatat ttatattaat
    18001 tcacatatct agcatatctt acttgccctc ttccagaatt aggttctatt tcaccaattt
    18061 ctattttaat aattacatta ttgtttttac acttttttga taattctgct gcagaaaggt
    18121 ttttaccagt aaaatctaaa gaaaatggct tatcatattc aaaaggatac tcaacaggta
    18181 caccatctgg agctaatgca accataccat atggtgtatt cataaaccct attacagaaa
    18241 ctgtatgcct tgatgggtct aaatttgaat ttttgcttgt aaacttaatt gtatttattg
    18301 atgagttgat taaactacac ggaactccct catacctttc aattttggga cgtagattat
    18361 ttccataaac ttcatcaagt attgcttctt cttgatctgc tatcatagtg tgattcccag
    18421 gtccaactgg cgcagaaggc ttagctggcg taggtttctt aacagttggc gttggattct
    18481 gtgtctttac tgattcacca acagctggat aggctttctt gtcaaatggt aatgggtgag
    18541 taagactctt gtatatgatg tctgctcctg ctgatttgtt ggcatcaata tctacatgat
    18601 acaaacgtcc atcctcaaaa cgaataagat tccactgatg gtcatttgtt gtactatgta
    18661 cataataaca ttcaattccc atagcatcac ataaagcttt caagctccta cacattccag
    18721 aacaagcacc ttttttagta accaatgtat aagcagtatt ggctccctct gctgcgttcc
    18781 agttaacata tgttgtgttg tttttaatgt gattataagc aaccctaatc ttagcatctg
    18841 tggtcatgtt ctttgtaatt ttcttgttca taaagtctgc tgttgcttgt gctaccgaat
    18901 taagttgagc ttgtgataac tttggaccat atatactgtt ggttccagct acatagcttt
    18961 tcggtgtctt tgcagtaaca tctttagctg tcataccata aactgttaca ctcatacatg
    19021 aggttaaaac catcgttgca atcaatagtt tagtagctaa tttcctgatt tttttcatat
    19081 tagcattctc ctttataata tattttactt tcaattccct accattaatt aagttcattt
    19141 tagatttaaa atatggatta tcaacatgtg tgcatagcaa ttcacgtggt catagaatat
    19201 ttagatttct tggaaaatct gttctatatt tcctatatag ttcgtacaca atccaactca
    19261 caaacctgca tttttgatat catcacaata aaatgataat aattaatata ttcaaaaatg
    19321 ttggtctgta acaggtttgt caattttcta attattggta atgacgagag tgaggagaat
    19381 tgtcaataat acatttaaaa ttaaaattgt atacacttca tacattctcc cctaacaaca
    19441 ttatacagat tataatttgt atattcaata cattttttgg aaggtataat tatatcaaaa
    19501 tatgtgatta tgtatagata caatccacta tggagaaata taaaagaaaa gagtataaca
    19561 aacaataaaa aataagatga ttcaaatttt attttgaaac atcttattta ctctacaaat
    19621 taaaattatt tggtgaatta tttaattatt attttttatt aaagaaattt taaatcttaa
    19681 tataaaatac gctattatac ataataatcc aaatgcaaat gctactggaa ttaacatctc
    19741 cgattcaaaa taaaagaaat attctaagaa aatatctgga tttactatat tcccattctt
    19801 gtctaaaaaa tcttttccat tatatcctaa atgctgtata aaattactta tgttattata
    19861 atctgcttcc ctctcatata ccatattagc attcaatcca gaagaactta aattagtgtt
    19921 tccatctttc atatctatta catatagtct aagagctttg actttactat acttatcttt
    19981 atcaagtatt ttttcaagtt ctaacttata ttcaccaata tttgtcatat catgtctttc
    20041 tgccaattgc tttatttctc catcaagttc ttttcctaaa ttatacatat tatgttgtaa
    20101 actattattg taataccaca taaatccaga aattaaaaat gcaattatac ttatgtaacc
    20161 taatactttt ttaaacccca ttacaattga tttatgccta tccaatgata aagatagttc
    20221 ttttattata ttacataatt catactgcat ttcatcacca tcatcaaatc tttcaattgc
    20281 ttttttacat gcttcttctt catctaatcc ttctaattta aattcatttg cagattctat
    20341 aagatggcat ctcatttcct gttttaattc tatagtactt ttattgtcac attttttata
    20401 tagtttatct aaatattcat ctattagttt cataaattaa tccacctctc ccaaaaattg
    20461 attcataacc tttttaataa atctccattc ttgtttcttt atttctaaaa aatctttacc
    20521 ttcattagtt aaattataat attttcttct tccacctgaa ctttgttcat tgctataata
    20581 agatttaatt aaatttttag attccattct tttaagtgca agatacattg ttccttcttt
    20641 taattcaaac tcacttcttt ctcttacttg ctttgcaatt tcatatccat aacaatcttt
    20701 cttttctaat atagaaacaa ttaatatatc gatataacct tttaacactt ctttatttaa
    20761 ctgcattatg taccaccgcc ttttcaccta ctactctata atgtatagta cttatattta
    20821 ctataataat acatagtact ctataataca aggtttatat tctacaatag acttaaaaat
    20881 aataaatagc tgtatattag tatttataga ttaaatacta atatacagct atttatcaaa
    20941 ttttaaaaat aaatttttgt tttattaatg ttgtcgtaac aacatttact tggttgaaat
    21001 agtattacct ataagtttaa atatatataa taatagactt aattctattt catattatta
    21061 aaatagagta atagtttaaa ttctactact ctatctataa tctatttcct aattatttaa
    21121 tctcaaattt agctcctcat catttaaatc tttcataaga tttctacagg tactaggttt
    21181 attgcaacag aaaaactagg gcattaaaca cttatatatc tgtttcttta gactgtaata
    21241 ttaatatttt ttaaatacgt tgacttttgc tttttctaac ttctctccat gatagttctc
    21301 ctctatcaag agcttttata aatatttctg ctgaagctag atttgtagct attggaacat
    21361 gataaacatc acaaagtctt attaacgctt gtatatctgt ttcatgagcc tgagatgtca
    21421 atgggtctct taaaaaaata actaaatcta tttcttgtgt tactattaaa gaacctattt
    21481 gttggtctcc acctaaagga ccagatgcta atctatttat atttaattca gtttcatcca
    21541 ttattctttt tcctgtagtt ccagttgcat atagtccata cttttttaaa attgattcat
    21601 aacctataca aaatccgacc atagtgtttt tcatttggtc atgtgctact aatgctatat
    21661 tcatatatct aatctcctca atttatgttt agatgtattt aaatttagaa atttattttc
    21721 tttcttctca tacacacatt tagtactaat cctaagtttg ctaaagatgt caataacgaa
    21781 cttcctccat aacttacaaa aggtaatgtt actccagtta ctggtattaa cgcaaccgtc
    21841 atacctatat tttggattat ctggtaacca aacattccca taacaccaac cactatgagg
    21901 gttccataaa aatcttttgc atctcttgca attgctaaaa gccttaatag gaatatcata
    21961 aataagatta tcaacactgc cattcctatt acaccaagtt cttcacctac aacagcaaat
    22021 ataaaatcac tatcttgtac aggtaaaaaa tcttcttgat tttgacttcc attgtaaagt
    22081 cctttaccag ttacgccccc agaaccaatt gcaatcagag attgcataac ttgatagttt
    22141 ccttttagag ttacatcttc tggatttaga aaagcttcta ttcttacctt ttgatggtct
    22201 gccatcatta aatacatcag tggcattgac actaatagta tgattattcc tctttttatc
    22261 aactttgagc taagacctgc tgtgaacagc attgcaaata tcatacaaca aaatacaatt
    22321 cctgttccca agtctggttg tgcaatcaat aatccgataa atggaagaga ataaactaca
    22381 acaggcataa cttcctttaa cgtatttaat ttatcctttt tggattcaag tatctttgca
    22441 taacttagta caaatgttaa tttgactatt tcagatgtct gcaagtcaag tggtcctaag
    22501 ttaatccacg acctagctcc accttttact gtcccaattc caggtaataa aactatggct
    22561 aacaaaataa gacttattat ataaagcgct ttataatacc tgcctagaag attatagtca
    22621 aaaaataata tgactataat cattcctata ccaagtacaa aagctaatcc ttgtttaatc
    22681 aattgattat atgaacctgt tgaattagca tgtgttgcac tactaagtat aacaagccca
    22741 aaaataaaga tagctaatac tgtaactata agtttccagt ctaactgttt tatcaactta
    22801 attgtcgact tataattaat attgattttt ttcaaagaat caccgtcttt ctatctttaa
    22861 acatataatt aaataaaagg ggcatatacc aataaggata tccccctttt aagcactaat
    22921 tacatacatc tatcttttat attctttagt ggtatattag caactaatgc agatacaggt
    22981 ttgtcatcat gctcacctct gcatttagac atttttattt caagaccatc atcttctatt
    23041 tctgcataat tagctattac ttttaaaatg tctgttttta tcatctctaa aagttgtggt
    23101 gaacaatcta ctctatcatg aactaaaact agctttaacc tctctttagc aacagattta
    23161 ctagttttag cctcgttaga aaaaactcta aataaatcta acacagatgt atcctccttt
    23221 gctacttagc catgctaaac atttttttaa gtctaccaaa gaatccatct tcaacttcaa
    23281 gatcaagtag aggaacttct tcatttagta tcctttttgc gatatttttg tatgcttgac
    23341 cagcaagtga cttagaatca agtatagctg gctctccttt atttgttgat ataattatgc
    23401 tttcatcatc aggaacaaga cctaacaaat ctattgctaa tatttctatt atatcttgtt
    23461 tatccatcat gtctccacgc ttaaccatat catttctaat tctatttata actaatctta
    23521 tctcttttat ttcatttgct tctaatagac ctattattct atcagcatct cttactgctg
    23581 atatttctgg attagtaact acaatagctc tatctgcccc agctactgca tttttaaagc
    23641 cttgctcaat acctgctgga cagtctatta ttatatactc gaaagattct tttagtttct
    23701 cacacagatc aatcatctgc tcaactgaga cagcattttt atctctagtt tgtgctgctg
    23761 gtaataaata tagattatca aatcttttgt cttttattag agcttgctta agcctacaag
    23821 ttccttcaac tacatctact atgtcataaa ctattcgatt ttcaagaccc attactacat
    23881 ctaagtttct aagtccaata tctgcatcca caactactgt ttttttattt tctaggctta
    23941 gcgcagttcc taagtttgct gcggtagtag tttttccaac cccaccttta ccagatgtta
    24001 taactataac ttcgctcata atactacccc ctatttttct ttctatttgt ctaactttga
    24061 caaatagctt tcgattacaa ttcttccatt acttacaaag gctatttctg ggctaatttc
    24121 tgattcattt tcataatttt cttcatcatc tggagcttct gcaatattat ctgctatttg
    24181 taatatttta ggattaagat ttccagcaac aacatatgca aattcattgc cttctgcacc
    24241 tgcatgtaca aaaccttcta ttttacccat tacaaaaaca ttacctcctg ctacaacctt
    24301 agcaccagca tttatatctc ccataacaac tgtatttgat tttgaaagga cttttgaacc
    24361 aggtttcata tcattcaata taactacatc accttcaaat tcaatatttt caccagatct
    24421 tagggtattg acatattttg ttggaaaatt actattttct tcaatttttt gatcttcaac
    24481 aaattccaca tcaaatctag aagtaattcc ttctttaagt tctagaatct ccatgtcagt
    24541 taaacaatca ctattaattt tactaatttt agccccatta aaaaagccaa catatgcttc
    24601 caatttgttt attatttttt cctgtatttc ctcaaatgga gcttctcttt ttatatttac
    24661 tattatccct cttttattac ccttgaactc tactagttct tgactacaaa tttctcttaa
    24721 agacataaca acctccagag tgatatatca tttatattct tcaaatacgc tttaaaccct
    24781 tttatttttt ctatataaag ctaaagttta tacatagatt aagctatcta ttaatttgtg
    24841 actcgaaatt tatattttcg tcagaagaat tgttaacatc attcgtcttg ttgtcatttt
    24901 tactattagt tggctctaat cccatataag ttccaataac ttctctagtt ggtaaaagtg
    24961 agaatacact tgaatcacct tgaggaatta cacatacaac agctatttct gggtcatctg
    25021 ctggagcaaa agcaacagtc catgtaaaag aaccataatc ttgtttaaat ctatctattt
    25081 ggtcatctgt tatttttgga tttaattctt ttatagcttt tcttagatat gaagaattca
    25141 ccttatcagt gtcctctaat ctaactttta ctccatcttc taattcttcc tctaatttct
    25201 ttctttcttc gtctttcaaa tctttatttt ctaatttctt ttttatttcc ttttccttag
    25261 ctaatgaaag ctctttttct ttttctgctt tcatcttatc agctagttta atcgcatcat
    25321 ttaggttgac attataagat gacatatgac tttttaaata ttcatactcg ttatctgtag
    25381 gtattttccc acttttctca gctgtaccag tttttgctgc tacatctatt ggaaaatcag
    25441 caaaagcact cttcgctgta ccttgtcttg caaccaattt catacctttt gttaattctt
    25501 ttaagttatc tggattttta aaaggtattt tttcgacttt cttctggtca ttaattttaa
    25561 ctgatgaata atcacttgaa acagctctat caactacaga taattctact aaattacctc
    25621 catttgctat agcagctaca tatcttgata tttgagctgg tgtataagca ttttcacctt
    25681 gacctatagc taagttaaat gtatctgcag tactccattt tgcaaagtta aaataactga
    25741 atactgctaa atctgctact tcttctattc tatcttcttt aacattcatt ttctttagcc
    25801 tattcattgt ttcgactcta ccaggagttt tcttttctgc tgcccagctt acaatctcat
    25861 tgattctttt ctcatattct ttaggatttt tttctctagt tatatcaaca aaatcatttg
    25921 ccatttctct ttctaaattt gactttagaa gagcttgtgt tgatttcaat ttatcttctg
    25981 tgctaggaac tttacctgct ctttcttcta cttcatcttg aagccctgta ttttggtcaa
    26041 gcccaaataa ttttgcatac tcaagtattt tatttgcatt catatctata ccaatgcttt
    26101 ttccatctgg ccatgtttta cctgtaccaa ttgttgccat atatatatta caagactctt
    26161 gtatcgcttt atataagttt gtcataccat ggtttcctct accatggttc catacatagt
    26221 caccaaaaga ttttttacct aacattataa ccccagggtc atttatagta aagtttggat
    26281 ccaaaccatt ttctaatgct gccattgaag ttagcatctt aaatgaagaa cctggctgaa
    26341 attccccttg tgtaaccaag tttaaaagtg gacttcctgc tagtaagtca tttggatttt
    26401 ttggttgaag tgctttataa tcctctgtag aaataccttt tgcaaattta tttgggtcat
    26461 aattaggata acttgatgaa gctaatactt ctccattttt aacatcaata gctataagag
    26521 ctgctgactg tgcttttcct gcatatgcac taattggttt gtccccaaat ttacttttaa
    26581 aagtacctcc tttagaagca acttcgatta tctgttttaa tgcattatct gaaacttctt
    26641 gtaaatcttt atctagtgta agatacacag tatctcctga tttaggtttt ttagattcaa
    26701 tttctttact gattctaccc aatgcatcaa ctttaaccat tttataacca tcagttcctc
    26761 ttaatctact ttcattactc ttttctactc ctgcaagacc taccatatct ccagtttcat
    26821 aacctttttg aaggtatgat tctatttgag tggatggcat ttttcctaca tatcctaaca
    26881 tatgagaagc taatgaccca ttaggataat atcttactgg ttctactgct attgaaacac
    26941 caacaaggtc catagcactt tcttcaattt gagcaatcgt agtttctcct acatccttag
    27001 caatagttac tggattatat tgagaatatc cttttgattt tatcaaatcc cttacgacca
    27061 ttatttttct tgcatcttca tcactcaaac ttttatcaat ttctaatgca tcactatttc
    27121 ttactttttc aaacgcttct ttggcactca gtttagtttc ttttatctta taactagcca
    27181 accaatctct cttatctctt tctgctgtga acatccattt actaactaaa ataggtggat
    27241 aatatccatt ttcatttagt tttgcctgta atcttgtcgc atccaatctt ttatcttctt
    27301 gagataatat tccagctgat ataagtttgt ctactaagta ataaaaactt tccttagcat
    27361 tataatcatt tggaatacca ttttctgatt tatattccct aatatctctg tcatatgtat
    27421 aatagtactt tccattttct acatatatag gaaactcatc tacatattct tcaccatttc
    27481 tctccagcaa atttataagt tttaatgata tttcatttgc tcttgaatgc ttatttgcat
    27541 ctttcttatt aatatcgtta cctgaaactt gaacagtaaa taagttttta tttcctgcca
    27601 acagtcttcc atatctatct tttatttctc ctcttggagc ttctattgcc aactttttat
    27661 atgttttatt ctcagctaac tcattgtaat attcatattt aaatgtagtc atatagatta
    27721 tttttactaa aatagctaaa aatatcacgc tcatgactat cttaattatt tttagtctat
    27781 caacttgttt attttcttcc atgaaatcac caactaatct tctttaagtt ttaaaactgc
    27841 tcttttagat aagcgatata ttactaaacc aattaaacta ttcactattg gtgctactag
    27901 tagacctttt acaaaaagtg ttgatatgtt gtagctttga taaattatac tagatgtacc
    27961 tatattgaca attgaatcaa ttaaactgcc tataaaaatt aaaacgaaag taataatata
    28021 actaccttta tatagctgat ctctcatgta tccaattgca tatgcaataa taaacaaaat
    28081 taatgcattt acaccaaaaa ttccaccaac ggttatgtct ttaataagcc ctattattgc
    28141 tgctataatc ccaccttcta attcatctaa ataaagagag attatggtta catatataag
    28201 aactaaatta aagcttatgc caaaaatatc tatataattt gttatactat tttctatagt
    28261 cataagcaca atccccaaca gacaaagtaa aacttttttc attgttttta tcccctttat
    28321 cctatatttc ttggttctat gactacaacg tcatttatat tcttaaaatc aacataaggt
    28381 tttacaacta catattttaa tgacttatct ttatcatcaa ccaccttttc aacttcgcca
    28441 attggtatac catctgggaa gaatccaaga cctgatgttg ttattatatc tccttgaatt
    28501 acattatatg atgagtcaaa catatatcct tgcaaatatc ctttactatt ataactatct
    28561 ttattatcta aagtagtatt ttgagtaatt gtacctttag cattggcatc ctttgctagt
    28621 ttaaagctta cagatgcctt agaatccaat agtgaaattg ccttactata attgttggaa
    28681 acttcataaa ctatgccaac taacccacta ccattcataa ctatactttc tttttttaca
    28741 cctgaatttt ttcctgcacc tatgacaaaa ctctcatacc agtttccatc atttttacca
    28801 acaacacttg ctgaaatact tgtcgcctta tactcttctt gtacaaagtt cagtgtcttt
    28861 tttaactctt ctagcgattc tgttttatct agtttggcat ttaaagctat catatctttt
    28921 ttaagttttt cattttcttc ttccaatttt tttacctttt tagaattatc tttaaaattt
    28981 gctacatctt taaaaccatt tttgataaaa gaaaatccat catttattcc cttacctaca
    29041 cttgtaatag catctgaagc tactcctaaa tttactggac taccactaga aaatttacca
    29101 attgatatac ccacaattcc aattaaagtg atagcaacta cgcccgttgc tatcacttta
    29161 acattaatcc tttttttctc atttttgttc ttgtcaaatc tcaaggccat caccaacttc
    29221 taattcttag tattcatcat taatactttt tcaaagattt cttggtcttc aacagattta
    29281 cctgttccaa gtgcaacaca atcaagaggt gtttctgcta tttgtacaga cataccagtt
    29341 tcctgtgtaa taagcttatc aagacctcta agcaaagcgc ctcctcctgt tagcattatt
    29401 ccgttttcca ttatatctga ggctaattct ggtggagttc tttctagtgt agattttatt
    29461 gcatctacta ttgaacttac aggttctttt aatgcttctc taacttctgt agagcatatt
    29521 tctattgttt taggtaatcc tgagattaaa tctctacctc ttatttgcat atttatttct
    29581 tcatcatctt taaatgtaga acctatgtta atttttacat tttcagcagt tctttcacct
    29641 atcataagat tatattcttt ttttacataa gccactatag actcgtcaaa ttcgtctcca
    29701 cctattctta tagatttagc agtaacgatt cctcctaagg atattacagc tatttctgaa
    29761 gtacctccac caatatcaac taccatatta ccttctggtt cttcaacttt taatccagcc
    29821 cctatagccg ctgccattgg ctcttctata aggaatgcat ctttagctcc agcttgtctt
    29881 gcagcctctt ctatagctct cttttctact tctgtaactc caaaaggaac acatatagct
    29941 attcttggac ttacaacacc ttttttatca gccgcttttt gtataaaata gctaatcatt
    30001 gattgagtta tgtcaaaatc agctattact ccatctttca taggtcttat agctactata
    30061 tttcctggtg ttctacctat catttttttg gcttcttcac ctactgctaa tacctcttta
    30121 ctatcatctc ttattgcgac aactgatggt tctctaacaa caataccttg tccttttatg
    30181 taaactaatg tatttgcagt ccctaaatct attcccatat cctttgtcat cttattaaaa
    30241 ctaaaaaaac cttttttttc tttctttttt tccttagcca tgattttact ccttcctact
    30301 attaagaatc atgtttaaaa cattctccta ttattttatc acatataaag tcaaattatc
    30361 atattttctt taaagctaaa gtataatcca tctccaataa ttatatggtc tattatttct
    30421 attccaatta tttctccaca tttaataagt cttgatgtga tatttttatc ttctcttgat
    30481 ggttctacac ttcctgaggg gtgattatgc atcactatta tcttacttgc acttcttctt
    30541 atagcttctt taaaaacctc tcttggatgg actaatgacg agcttaaagt ccctatagat
    30601 acatctacat cagatattat ctcatttttt gtgtttaaaa gaactgcttt aaaaacctct
    30661 ttttttaaat accttaggct atccatataa tacctctgta tatcccaagg attccttatt
    30721 ttatacttta taggtttaaa actagctact ctagagccta gttctaaagc tgctttaatt
    30781 tgggttgatt ttgataaccc aattccatct atattacaca gctcctctat tgtcatatct
    30841 tctaaatgtc taataccctg aatatctcta tttattatat agttggctaa ttcaatagca
    30901 tttttattct tgttgccagt tcttaaaagt atagccaaca actcagcatt tgataagctt
    30961 tttacgcctt tggcaagcat cttttctctg ggtctttcct ctggtgccat ttcttttacc
    31021 ttaatggttg aattaaatga ctttttcaga taaatcacac tccataaaaa agatttatac
    31081 taaaatattt ttttaatagg tcgcctaacc ttgaaattgg aagaccaact atattaaaat
    31141 aatctcctct aatttcttca actaacaaag ctccataacc ctgtatacca taggctcctg
    31201 ctttgtcaaa tgactcatta gttttcagat aatcctcaat atcctgctct gataacgttt
    31261 taaatttcac attgctgatt acatagtcaa ttaccttttt atcttcgcac aagtttatta
    31321 agctaatacc tgttatgact tgatgctctc ttccagatag tctttttaac atatctcttg
    31381 cacaagactc atcttttggt tttcctaaaa tagcattgtc taaaacaaca actgtatcag
    31441 ctccaataac aacatcttca ttatgttcag atgctacaga catacttttt tcaaaagcta
    31501 atctcataac taaatgtttt ggtgtttcac cttccaatat aatctcatca atttcatttt
    31561 taattatatc aaatcttaca ttcgtattct ctagaatttc tttccttctt ggagaggctg
    31621 atgccaagat tatattcatt tattcacctc atttgtacgt aatttgtacc tatttagtct
    31681 atcactatat tgtaaaaaat tcaacagaaa tgtcgaacct tttttgttac actattgtta
    31741 tatttattac cattagaata aatatacata aaatagaggt atggaaatta ttccatacct
    31801 ctgttttatg tactcattag agatatggaa attattccat atccttattt tgtccatatt
    31861 acaaaatttt aatagctttc tttggacatt tttccataca taaatggcat cctacacatt
    31921 tatctgcatc taccttatgt ttttctttta attctccctc tattgcatca aacttacatt
    31981 gttttttgca cactgtacat ccaacacaaa gttcttggtc aatagtaact ttctttttct
    32041 tagttatgtc acctgatatt acttttgtag ggcattttgc tacacattgc atacacccta
    32101 cacacttatt agggtctatc ttagctattt tgtcttcaaa tattattgca tcaaatttac
    32161 aagcttttac acacattcca cagccaatac atccagcagt acatttttcc ttaacagctt
    32221 taccaaactc tttgctatta cactctacaa ctacttcttg actttctggt tttgtgatta
    32281 ttataccctt aggacaaact tctttacatt ttccacaatt tacacacttt tcttcatcta
    32341 ttactgctat tccatcaact atagaaatag catcaaattt acaagcatcc ttacatgttc
    32401 caagacctaa acatccaaat ttacacatct tagcacctga gtttaaaaca tttgctgctc
    32461 tacaatctga tattccttca tactcatatt tatcttttgc acttgaacaa gttcctttac
    32521 aaataacttt tgctacttgt ttctcacctg cttcagcact tattcccatt atttctccaa
    32581 cttttgcacc aacatctgct ccacctacag ggcaaccatt aactggtgca ttgccttcaa
    32641 ctatcgcatt tgcaagacct ccacaacctg gaaatccaca acctccacag ttggctcctg
    32701 gaagtactcc tagtatagct tccactctct cgtctacttc tacagcaaac tttttagatg
    32761 cgaaatctag tactatcccg aagattaagc ccataattcc taaaactaat acagctgtaa
    32821 gtatcaccat tttcacccct cttttttata gctttataag tcctgtaaat cctaagaaag
    32881 ctattgacat taaacctgct gatataagcg ttatagggaa acctttaaat gcttctggta
    32941 tatcagctaa ctctagtctt tctcttatac ctgcgaatat tactattgct aaagtaaatc
    33001 cagctccggc tccaaatcca tttattattg tttctactaa attataactt ttttgaacat
    33061 taactagagc tataccaaga actgcacagt ttgtagttat aagaggtaaa taaactccta
    33121 atgcttgata taaagatgga ctcatctttt gaattaccat ttcaacgaac tgaactatag
    33181 aagctataac caatataaaa gctattgttt gtaagaatcc agttccagtt tttactaata
    33241 aaatctgtat aaaataagtt ataattgaag ctagagttaa aacgaacgta accgcaactc
    33301 ccatccctac tgctgtatca accttcttag aaactcccaa gaaaggacat atacccaaga
    33361 attgagatgt tataacgtta ttaacaagaa cgatacttaa aaataaaagt attaaattca
    33421 tttattacac ctccactatg cttttttact tcttaattta ttaaatcctg caaataagaa
    33481 tcctaatgtt aagaatgcac ctggtggtaa tataaatatt aatactggtt ggaaagatgc
    33541 tccaaataat gtcattccaa ataaactacc atttccaagt aattctctta ctgcacctat
    33601 tattgtaagt gcaacagtaa atccaagacc ttgacctaca ccatcaacta ttgaaggcat
    33661 agctcctgtt ttgaaagcaa aactttctgc acgagctaat attaaacagt taacaactat
    33721 taatggtata tatataccaa gagccttatc aagagcagga acatatgctt ttaaaagcat
    33781 acctactatt gtaacaaatg tagctataac tactataaat ataggtattc ttatcttatc
    33841 tggtgtaatt tttcttatta aagatataac taaatttgca catattaaaa ctgctgctgt
    33901 tgaaagaccc ataccaattc catttattgc tgaagttgta acggctagtg ttggacacat
    33961 acctataact tgaacaaatg ttgggttctc atctattagc ccatttttaa atactttagc
    34021 taaattcata attaaccctc cttatttatt aagagcacta ttgaaaacat ctatagcagc
    34081 atttactcct gtagtaactg ctgtagatgt aatagttgca cctgatattg ctaatatttg
    34141 attatctgct gttgtacttc cttttgctac ttcaagaggc tcagcagttt ttcctttgta
    34201 ttgaccctta aatgcatcat ccttagcttt tgctccaaga cctgctgttt ctgacatatt
    34261 accaatgtca acacctgtaa cttgaccttc agatgatata cctaccataa cttcaatttc
    34321 tccaccatat ccacttggtt tagcttttaa agtatatcca accacttcag aaccatctaa
    34381 accttcatac acttcagcta taagaccatc tccaaggtct ttatatgtac ttttatccat
    34441 ttctttaaat tcttttgctt ctggaaggac tgcttttcta agttcattat tttcttgtat
    34501 atttctttgt tctattactg gagctgtaac ctgattagta gctccaagag ctaatgcagc
    34561 tattgcactt atagcaagta aagtaccacc taatctcacc atactgttca ttatttagcc
    34621 acctccccaa atactctatt tctaacaaac ttatctataa gtggagcaag acagttcact
    34681 aatagtattg agtaagatac accttctgga tatccaccat acattcttat aacagttgtt
    34741 ataagaccag ctaatacagc gtatattatt tgaccttttt tagtcatagg cgaagttgta
    34801 taatccgtaa gcatgaagaa tccacctaac ataagtcctc cagcaaacag ttgatatatt
    34861 gcgaaagtta tattaaatcc acctaaaagg aatgttaata taaatacagt tgctatgtaa
    34921 aatactggta taacataatt tattatgcct ttatacatta ggtatactcc acctaataat
    34981 aataatattg ctgatgtctc acctatacat ccaccaatat tccctatcat catgtcagca
    35041 agactaattc cattgctaga aaggtctgcc agtcctgctg ctccattttt caaaaagctt
    35101 aatggagttg ctgtagtaac tgcatctaaa tttttaccta tccagttaac acctggttga
    35161 gtccaagcag tcattgctac tgggaatgat gctaataaaa atgctcttgc agcaagtgct
    35221 ggattgacaa agttacatcc aattccacca aataccattt taactactaa tattgcaaat
    35281 attcctccta ctagacacat ccaccaagga agtgatgctg gtacgttaaa tgctaataat
    35341 aatcctgtta caacagctga aaaatctcct atagtacttt tttgcttcgt aaatttttga
    35401 tataaaaatt cagtacctac tgtaccaagt atacaaaaaa acatagcact caaagcatta
    35461 agtctaaaga agtatactcc tgctactgct gctggaagga gtgctataat aacttgtttc
    35521 attatatatg aagtatcttc attacttctc acatgaggag aagatgatac tatcaactta
    35581 ttttccatcg tttttccccc ttaactttct ttatttctga gcagcttgct ttcttctctt
    35641 tgctcctatt tctctctttg ctgatcttat agattgaagt aatggtcttc ttgctggaca
    35701 aataaatgaa caagacccac attctataca atccattgct ctatgatatt cagctgtgtc
    35761 atagtcatct tttaaagaat atgcacttat gaaaagtggt tgtaagaatg atggacatac
    35821 atctgtacat cttccacatc ttaaacagtt ttgaatatct ggagtacgtg attcttcctc
    35881 atccaagcat aaaattcctg aagaaccttt gttagtcgct atttcagtag tatattgagc
    35941 tattcccatc ataggtccac ccattataac cttaccaatc ttcttatctt ctttaaatcc
    36001 accacattgt tctattattt ctgaaactaa tgttccaact tttgttatta ggttctttgg
    36061 ctctttgatg caactacctg ttattgtagt gattctttca accaaaggca tacctgtttt
    36121 aatagactta gctatttgtg ctgctgtggc aacattgtca acaactgcac ctgctgctat
    36181 tggtaatcca ccagatggaa cttctttgcc tgtacacgca taaataagtt gtttctctgc
    36241 tccttgtgga tatttcactt gaaggccaac aacttcgatt tcactatagt ctttagctac
    36301 attttgaatt gcttctatag catctggttt attcgtttca attcctataa atcctttttt
    36361 gacatctaat accttcatta atgctcttaa accaaacaca acatcttctg gattttcaac
    36421 cattaatctg tggtctgcag ttaagtatgg ttcacattct gcaccattta atataactac
    36481 ttctgccttg ctatctggtg gaggagatac ttttacatgg ttagggaatg tagctccacc
    36541 catacctact attcctgcct cttttatgat acctactata tcttctttgc tcaaatcctc
    36601 taactgacct tttggttgaa cactctcatg taattcttcc ttaaagtcat tttcgataac
    36661 tacacattga gctgaacctc ctggtacttc atgttgttca atagcaataa ctttacctga
    36721 tactgaagaa tgaacgttac atgaaacaaa accttgttgt tcacctattt tttgcccaaa
    36781 ctttacttca tcaccaactt caacaatagg tttagctggt gcccctatat gttgctgaag
    36841 aggaatgtaa actatctttg gagcttgagc tttttcaagt gcctttgtgt tagagtactc
    36901 ttttctatat ggaggatgta tccctccctt aaaagttaag agtttcattg ttttaccccc
    36961 ttatctataa aaaaacattt tttctgcctt ctataagtat actatatata ttaaaaaatc
    37021 ttaacaattg ttaaaaaaat atccgtattt ttataattta ttttataaat caatacctct
    37081 atattaagtc aaactgagga aaaggattgc ttaattaaac aatccttttc atatagcttt
    37141 ctaaagtcca aattttctgt attttatttt atagaattta taaacataga gtactcctgc
    37201 atggatgata aaagcagact tttacttaca ctatattttt cttcatttgc tgcttttgaa
    37261 gctgtatcaa ttttttcatt cacattcttt acatactcta ttaaattatc tttaaataaa
    37321 cgcatttcag aatagtcaat cttttcagct tcttttgata tattttgtga tattgccttt
    37381 ctatttgtca aaatagtatt atatttttcc atatctacat tattttcagc attcgaccaa
    37441 aaacttgatt cttctttaaa gtttgttatt aatttattta attctttaga tatactctct
    37501 atataagcgt agttacttgt atactctaaa gacaacatag gtgcatctaa gtgtgataaa
    37561 aatgcatctg gaaacacctt tcttacactg cttatctgct ttctaacatc attttcatca
    37621 aaagaggaat aggtctgtat tttttttact ccatccttct ccataactga aaaaggtatg
    37681 tcattattaa ctaatgatgt ctgtattttt ttcaaatcat cattattatc tattgcagca
    37741 acttgaatag tgtacatatc ccagccttct atagtagcta atttgacttc ttctggctct
    37801 ttagtgttat ttaccgcttt ttctggatta gtttcttgtt tatcatctga attggtttgt
    37861 gatttagatt ttttcccttt ttttattgaa tttaattcat cagatatatc tttataagtt
    37921 atatctgatg aattatttag aaatggtatt ttagccgata catactccaa tatcttgcta
    37981 tcttttattt ttgtataaga ataatagcca attagacaca agcaagcaat cgttataaca
    38041 tatttacgta aatcaatctt atgtctatta ttattaaatc ctctgtatat cgtttttctt
    38101 ctattcatta gtttgtccct cccaaaatta tattattaat aaagtatatt tttaagagtc
    38161 acaatttatg aatataaata attatttctt ctaatttttt aataagaaat tgtacttact
    38221 aaacaaatct tcttgcacca agtaatactt tagaccaata tgaactagtt aaattatcca
    38281 ctttaacctt attaggatta gtctgagtac cactacaatg gataaattta ctatttccta
    38341 catatatacc tacatgatta gttgttccat caccatcagt gtcaaaataa actaaatctc
    38401 ctggtgcata attccccata gaaattgcac ttccaaaatt agcctgttct ctagaaactc
    38461 taggaataga aacgcctaca gaatgtttaa aaacatactg tgtaaatcca gaacagtcaa
    38521 atttatcagg tcctgaagct ccccattgat atggtattcc aataagagta tatgcaaaat
    38581 ctacgattgt tgaacctttt gctgtagatt tatctgaatt tccagtaaat gatggctttg
    38641 agttactatt agattgagaa ttttggttag atgatgaatt tggtttttct tttgtacctt
    38701 cattagtctc agaaatataa ctggcactca cccatccaat tgttccactc gataacttta
    38761 ccttatacca accattattt ttggatttta attcaacaac atctccacca tttaattttc
    38821 ctattataga atagcttgtc ccaggaccac ttcttacatt taaactcaca gcagatgtta
    38881 ccttaccatt cttgccttca attgattctt cgctagatgg ttttttatca gaattattag
    38941 tactatttga attactagaa ttattcgttc caacatcctc tgatgtttta gagatatatt
    39001 ggctactagc ccatccagta actccatttg atagttttat tttataccat ccattgcttt
    39061 gttctagtaa ctttactaca tctccattat ttgctttacc aactaatgaa tagttagtac
    39121 ctgcaccact tcttacattc agtctagaac tcacatttac tttaccattt ccactaatag
    39181 ttgtcccact tgaagttgga ttattttgat ttgatgtacc ttcagatgaa ccacttgttg
    39241 atatgtattt tgcgcttccc catccaacaa cagaactcga tacccttact ttataccatc
    39301 cattagactt ttctaaaatc tctacagttt ttcccttgta taattttgct accttatcac
    39361 aatcagaact tggtccactt cttatattca aggcacttgc tgttactgtt cccttttcaa
    39421 gagcacttgc atttatggag ctaacagata ctgctactgc cccaattcct aaagctgcta
    39481 tagctttctt cactactatc actcccccca acaaattttt atagtcataa attcataaca
    39541 ttattatcca tttttagtcc ctacaattta atgataactt attctcttat taattgtaaa
    39601 gaaattataa acatatcctt aaaattaatg atttttagga agtatttcaa tccaataatt
    39661 atctgggtcc gctataaaat aaattcccat actttcattt tcaaaacata tgcaatctaa
    39721 atctttatga agtttttttg cctcatcaaa ctcctcaact tctaaagcca gatgaaattc
    39781 attgtctcct aaattatatg gtctgtccca atctctaagc caagttaatt ctaatgtatg
    39841 actagtaata ccatctccta aataaactaa tataaaactt ccatcttctg cttccttacg
    39901 ctttacctct ttaagcccta aagctttttt ataaaaatct aaactttttt ctaagttagt
    39961 aacattgaaa ttgttatggc aaaaattaaa cttcattttg aaatcactct ccaattttta
    40021 ttttttttat attatatcat tattataaat ttatttaaat catattaagt taaaaatttg
    40081 aatgatgatg accaattacc aaatggtggt aaattattta aaagaatatg tcaaaaaaag
    40141 aataagctct ttcaaatgca caaaaaattt atacccaagt tatacttaat catgcaaatt
    40201 ggcagaactt attctttaaa actctaactt ttaagttttt ctttgataat ataatttatt
    40261 actttttttc taatatcttc agcattataa tcatcttcta cttctatatc caaaagtttt
    40321 gctattgacc tcatatcttc tctagtcttt gacctatagc ttgatattaa ttcatctttt
    40381 attttctctg gttctaaaag atattcttca tctcctcttt tgaaaacaac gtatttttga
    40441 gcatatgaac ttctattata gtatatatat cttgatattt ttcttaaaat ttgattatat
    40501 gaaagtcgct tgttaatatc aagttttaag tactctgaaa aagcaaatag ctcttttttt
    40561 gatttaaata atgcttcttt ctttatagtc cttgatattg aagttacact ttcttgatat
    40621 gcacactttt ttaacatttt taaatatact ctattaagac caatactttg attgaatttt
    40681 tttatattat tattaataat cttatccaag ttaccttcaa agtatttttt tgctcctttt
    40741 tgtgatacat aactctcaag ttctttgtgg gccatatcaa gtatcatttt ttttaaatcc
    40801 tctttatatc ccctagattc catgatactc cctcccttag ctatttatag tatattcaaa
    40861 aagctcaaga aatgtgagtc attacaaaat ttttttactt tttatgtata tattaattta
    40921 aaaggggaga taatagtaat gtaaaatcaa acatactcaa tctcccccat ttttagacct
    40981 ctattactcc cccaatagag gccttttttt agtttatcaa tttctattca attcccttta
    41041 gttcaaatcc taattctgct atcacttctt ttaatttttc aatagatata tcatctttca
    41101 tatctacaga tgcacactta ttttctaaat ccacatctaa aacttcaata ccatctatat
    41161 cttctgttaa tgcagtttta agatggttta cacaatgacc acaactcata ccttctacta
    41221 atagtttctt cttcatttaa ttacctcctt ataccccata agggtattct atcatatagt
    41281 ttatactttt taaatagaaa ttaatcattt aatttgattc attcgatttt atatctaaat
    41341 tagatattcc ctcatataca tcaaaatcat ttggagtaat tttacctttt aatctactat
    41401 tacttacaat atagttgttt ctcacagcta aacttataaa tgttgcatta tcttttatat
    41461 atgtctgtac atctttgtat gctttttttg tttcaatttc agacgtagaa cttgtaagtg
    41521 cagacatata tccatttagc ttatcatcag tatatccact acttgctata atactacttg
    41581 catctggtat aattgatagt ttccatccaa ctaatgctaa atcataattt ttagaattaa
    41641 gagcattttc catatcctta tctgacagct gatttactgt actatttatt cctatagctt
    41701 ttaagttttc atttattatg tttgcagttt ttattctctc actattatct ctgtttacta
    41761 ttatctttaa gttcatttca gaaagacttt tctttacttc ctctttttct ctattttttt
    41821 tcttttcagc ttcttttttg attctatctt cttctgcttt tttagctttt gcttcttctt
    41881 ctggcgtaag cttctttgag ttctttttag ctgatttttt ctgatttttt actttattgt
    41941 cattttgatt tactgagttt atatttgcat attctacctt agcaagacac tctatcgcct
    42001 tatctttatt aaactctaaa tccttcattt cactattata gtattttgat tttgaattaa
    42061 gtggaaaatt aactggagta gcatcatcca tataaccttc actaataatt ctatctttat
    42121 ctatagagtg agcaattgct tttctaaaat taacatcttt aaaaaatgga ttattataat
    42181 taaataatac acactcataa tctcttcctt gatattttgt tatattaaac tctttttctt
    42241 gaaacttgct taaatcattt aatgtaacat tggttatatc actatcaaga gccataacca
    42301 tagatgtcct tgcctcttca ttaggcacca tacctacttt tatgttcttc atggtctttg
    42361 gaacttcttc atagtaatct ttgtttacag ataaaaccat tccttctctt tctgtatatg
    42421 attctatttt atatttccca tttcctatca tattattatt cttgtcattt acatcctttg
    42481 aatctaaatg attttgagat acgattggaa aaattaaact atcaattgaa aaagcatatc
    42541 tagctttaaa ttttattgta aaatctttat ctgatttaat attcacagaa gctatatttt
    42601 tagtaaattc attatatgga ctatctgcac ttttttgaat taaaccaata gtatagttta
    42661 catcatttga agtaacattt tttccatcat gccactttgc atcttttagc tttatatcta
    42721 tactcattcc atcctgagct atattatact ccttaactaa ttgaggaatt acattgtagt
    42781 tctcatctat agtaaataaa ctatcataaa ctaaattcat tatataacca acagacttat
    42841 cagtattagt tataggattt atagtttttg gagttaccat tgttaagttt atatacttag
    42901 catctataga actttgactt tgtaaacttg aatcactact actttgtttt gttttagtat
    42961 tactacaacc agctaccata agagttatag ctaaaacgac tgttaagact tttattctct
    43021 tcattagttt tttcctttct tgccatatat cttattataa tgttcgtaat tattttttac
    43081 tttttctaca tattttgaag tttgcttaaa aggtatatca tgtagatttt cgccatcttt
    43141 actatattca ttattttcta accatttttt cacattacct gaaccaccat tatatgcagc
    43201 tatcactaaa tctaatttac caaattcttt gtataactta cttaaatacc aacagccaag
    43261 tcttatatta gtctctgggt cgaaaatatc cacatttttt aaatttagtt cctctgctcc
    43321 ccaatctcta gttatatcta atatttgcat taatcctttt gcttcctttt ttgaaacagc
    43381 agaactatta aacttacttt cggctttaat aacactgtaa actatatttt catctaaatt
    43441 aaattctttt gaatactttt ctacatactc tgaatatttt ttaggatata aaaatttatg
    43501 tattactttg ctttccatta atagtgcccc aaataagatt ataaaaatag atagaatcaa
    43561 tactttctta ctattcacgc caattctcct tcatgtactc aataaattta tgtgctttac
    43621 tttctaattc agttattgtt cccgaattat ctattatata atcaccatat ttggattttt
    43681 cttcctgact catctgtgaa tttatcctac taagtgcttc ctgcttacta caattatctc
    43741 tcttttgtat tctactaatt tgtacttctt gtttgcaagt aactactaat agcttgtcaa
    43801 ccatatctaa aaaaccactt tctaccaata ttgctgcatc taatactact atattttcgc
    43861 ctttttttct caattcttct atttcactta ttattttttc tcttattcgc ggatgagtca
    43921 agctatttaa ttccttaagt ttttcttcat cgctaaaaac tattttccca agagcttttc
    43981 tatttagagt tccatcatca ttttttatac tttgcccaaa atggacaaat actttttcca
    44041 ataacaactt atcctcaaat atcttcctgg atataatatc agcatccact atagaaatat
    44101 taagatttct aaatatattt gataggctgc tttttccaca gcctattcct cctgtaagtc
    44161 ctaatatcaa cataaaatca cctactttgt ctcataccaa gaactaccta catttaaatc
    44221 tacatctaag tgaacatcca tatttacagc attttccatt tcttctttta ctattttctt
    44281 aactatatct atttcatcat caattgcttc aactatcaac tcgtcgtgaa cttgtagtat
    44341 aagttttgat ttcaaattgt tctcttccaa ttttttatga acatttatca tagcaatttt
    44401 tattatatct gctgcacttc cttgtattgg tgcattcatt gcaaatctct ttcctctatt
    44461 tctttccata aaattgcttg actttatttc tggtatatat cttcttctgt ttaaaattgt
    44521 tacagcatat ccatcctcac tagccttttc tactgttgtg tccataaact cttttatttt
    44581 atggtattta gcaaaataac tttcgatata ttctttcgcc tttggaacag gtatatttaa
    44641 atcatcagct agaccaaagt cacttttacc atatataata ccaaagttta cagctttagc
    44701 tgcacctctc aattcactag taacatcttc cataggaaca ttaaaaactt gtgaagctgt
    44761 tttggtatga atatcttcat gatgtttaaa tgcttctatc atattttcat cctgactcat
    44821 atgtgcaaga actcttagct ctacttgtga gtaatcggca tcaaccaact tacagttatc
    44881 atctgcaata aacacttttc ttatttttct acccatttcc atttttatag gtatattttg
    44941 aagatttggt tctgtagatg aaattcttcc tgtagtcgtt atagtttgat taaatgatga
    45001 atgtattcta ttactttctg gatttattat atttaaaagt ccttcaacgt aagtagaatt
    45061 taactttact atttgtctat actcagttat cttatctatt attggatgct tatctcttaa
    45121 tttttctaaa acatctgcat tagttgaata tccagtttta gtttttttaa ttattggaag
    45181 ctctaatttt tcaaataata taacacccaa ttgttttggt gaatttatat tgaatggctc
    45241 tcctgataat tcataaattt ccttttctag tgtagctatt atttctttaa actctacact
    45301 caattcatcc aatatacttt tgtctacttt tataccttca tactccatat gacctaagac
    45361 ttctactaaa ggcatctcta catgataaaa aagaccatcc atactcatat ccattagact
    45421 tctttccatc ataggaattg tttctttaac tgtgcatatt atttgaccca tatactctgc
    45481 taaatcctca aattctaaat cctcatagtt tttagattta acaccctttc ctaataattc
    45541 ttcttttgat ttaacctttt ttgataaata cttcatagct attgcactac aatcatatga
    45601 ggtagatgaa gttgaatcta ttaagtactc agctattgtt atatcaaaat acatattttc
    45661 taagtttata ttatatggtt ttaaagatat ataatcatct tttaaattat atccatactt
    45721 tttaatttca ttatttgaaa atatactatc taactttgac acttcatttg aatctacata
    45781 atatattttt tctccatcta cacttataaa aatatatatt atattttttt caagtatatt
    45841 accttgtcct ctaacagttt ttaaaataac ttgtcctttt ttgtttactt catctataaa
    45901 tttttctgta tcttttaatt ttaatatttc tattttttct tctacgtgtt ctgtgtcatc
    45961 tgaatcgacc aaatcaacta atcttgataa gagacttgta aatccaaagt atctaaattt
    46021 atctaaaagt tcatctttat cataatcgcc aaaaaccata ctatctaaat ccacttctat
    46081 tggcacatct cttataattg ttgcaagtct tttactttga atagctattt ctttattttc
    46141 ctctattttc ttttttacac tacctttaag cttatctgta ttctctatta aattctctat
    46201 actagaaaac tcttttataa gctttatacc tgttttttca ccgattccag gtactccagg
    46261 tatattatct gacttatctc ccataagacc ttttaaatca ataaattgtg ctggagtcat
    46321 ctcgtatttt tcaattacag aattaaagtt gtattcttca acttctccaa ctcctttttt
    46381 tgttataaga gtagttgtat tatctgaagc aagttgaata gcatctttgt ctccagttac
    46441 tatgtatacc ttatacccat tattttctgc ttttttagaa acagttccta taatgtcgtc
    46501 tgcctcatac ccatctattt ccaatctatt tattttgaat ttatctaaaa ggtcttttaa
    46561 tggctgtaat tgttctgcca gttcatctgg cattcccttt cttccagcct tatactcttt
    46621 atattctaaa tgtctaaatg taggtgcttt tctgtcaaag gctacactta tatgtgttgg
    46681 tttatatata tctatcattt taaatagcat tgttgtaaat ccatatattg cattggtctt
    46741 taacccttct ttattattca tttctggcag agcataaaag gctctgttta ttattgaatt
    46801 tccatctatt ataattaatt ttttttccaa attatatcac tcctaaaaat tatctatttt
    46861 tgtaacatat atattatata ccaatatagt gttatttaat attcccataa taataatagt
    46921 atattaaaat taaatatgtt gcaaacaata tatttttttg ctataataat atacatgaca
    46981 tgcccgaatg gttgaattgg taaatacaat ggattcaaaa tcaactgtgt tgattcataa
    47041 gtttttataa tgttttacag tttgatgagt attttgtatt caatattcct cttgcatatt
    47101 cttggaaaat ctggtaggat atacaagtag tttggattta aaatataagc cgctgtggcg
    47161 gaactggcat acgcatacga ctcaaaatcg tacgggaaac catatgggtt cgagtcccat
    47221 cagcggcacc aataaaaaaa actctttagt atatactaaa gagttttttt attgtaatat
    47281 tctttcatct ttatttctag ccttgtataa gtataaatac catagtcata acttcttgaa
    47341 ctcctaaaat atatctaaac aaagtaggat tttttatgta ttcacttaat aaatccatac
    47401 ttcttatcat attgattttt tcccaatctc taaaaatttt gatacaaata aaaaatacag
    47461 agaactactt gatatactag aaggttattc tgtaccattt ggtattgctg taggtgttcc
    47521 agatggaaat ttaccaaatg ctagagatgt agtttataaa gtttctagag tttaggataa
    47581 agtgttgcaa caatcaatta gttacttgaa gagttaaatc agtaatatac aaataaatta
    47641 atggaagagg aagctattta aaaagtagtt tagatttttc attgtaaaat aattaaataa
    47701 ag
//
LOCUS       DAEPOZ010000005        44983 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.005, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000005 DAEPOZ010000000
VERSION     DAEPOZ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44983
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.005"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(303..653)
                     /locus_tag="KR440_000239"
     CDS             complement(303..653)
                     /locus_tag="KR440_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HBH0697309.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(669..1085)
                     /locus_tag="KR440_000240"
     CDS             complement(669..1085)
                     /locus_tag="KR440_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HBH0697310.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(1104..1295)
                     /locus_tag="KR440_000241"
     CDS             complement(1104..1295)
                     /locus_tag="KR440_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HBH0697311.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(1446..2261)
                     /locus_tag="KR440_000242"
     CDS             complement(1446..2261)
                     /locus_tag="KR440_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBH0697312.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(2308..3246)
                     /locus_tag="KR440_000243"
     CDS             complement(2308..3246)
                     /locus_tag="KR440_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH0697313.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSVGVDKIGIAIDASNKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYIYLKENDVTISLFAFTPVRGTKMEKIN
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(3243..4115)
                     /locus_tag="KR440_000244"
     CDS             complement(3243..4115)
                     /locus_tag="KR440_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH0697314.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEK
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(4140..6068)
                     /locus_tag="KR440_000245"
     CDS             complement(4140..6068)
                     /locus_tag="KR440_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HBH0697315.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNSL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKETLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(6180..6557)
                     /gene="gcvH"
                     /locus_tag="KR440_000246"
     CDS             complement(6180..6557)
                     /gene="gcvH"
                     /locus_tag="KR440_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBH0697316.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(6644..8770)
                     /gene="cdhC"
                     /locus_tag="KR440_000247"
     CDS             complement(6644..8770)
                     /gene="cdhC"
                     /locus_tag="KR440_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HBH0697317.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(8827..9633)
                     /locus_tag="KR440_000248"
     CDS             complement(8827..9633)
                     /locus_tag="KR440_000248"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBH0697318.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(9683..11050)
                     /locus_tag="KR440_000249"
     CDS             complement(9683..11050)
                     /locus_tag="KR440_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HBH0697319.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(11081..12025)
                     /locus_tag="KR440_000250"
     CDS             complement(11081..12025)
                     /locus_tag="KR440_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HBH0697320.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(12045..12815)
                     /locus_tag="KR440_000251"
     CDS             complement(12045..12815)
                     /locus_tag="KR440_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0697321.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(12871..14256)
                     /gene="lpdA"
                     /locus_tag="KR440_000252"
     CDS             complement(12871..14256)
                     /gene="lpdA"
                     /locus_tag="KR440_000252"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBH0697322.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVIEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(14300..15181)
                     /locus_tag="KR440_000253"
     CDS             complement(14300..15181)
                     /locus_tag="KR440_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBH0697323.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMQKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(15217..15891)
                     /locus_tag="KR440_000254"
     CDS             complement(15217..15891)
                     /locus_tag="KR440_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HBH0697324.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(15928..16800)
                     /locus_tag="KR440_000255"
     CDS             complement(15928..16800)
                     /locus_tag="KR440_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HBH0697325.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLVKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(16892..17524)
                     /locus_tag="KR440_000256"
     CDS             complement(16892..17524)
                     /locus_tag="KR440_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HBH0697326.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKIEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(17629..19305)
                     /locus_tag="KR440_000257"
     CDS             complement(17629..19305)
                     /locus_tag="KR440_000257"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBH0697327.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(19554..20333)
                     /locus_tag="KR440_000258"
     CDS             complement(19554..20333)
                     /locus_tag="KR440_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0697328.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(20643..22562)
                     /gene="cooS"
                     /locus_tag="KR440_000259"
     CDS             complement(20643..22562)
                     /gene="cooS"
                     /locus_tag="KR440_000259"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBH0697329.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKAGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(23169..24080)
                     /locus_tag="KR440_000260"
     CDS             complement(23169..24080)
                     /locus_tag="KR440_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBH0697330.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(24196..24318)
                     /locus_tag="KR440_000261"
                     /pseudo
     CDS             complement(24196..24318)
                     /locus_tag="KR440_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
     gene            complement(24401..25543)
                     /locus_tag="KR440_000262"
     CDS             complement(24401..25543)
                     /locus_tag="KR440_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBH0697331.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYKVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELGINK"
     gene            complement(25561..27315)
                     /locus_tag="KR440_000263"
     CDS             complement(25561..27315)
                     /locus_tag="KR440_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HBH0697332.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMKINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKQSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            27451..27657
                     /locus_tag="KR440_000264"
     CDS             27451..27657
                     /locus_tag="KR440_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697333.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(27662..28585)
                     /locus_tag="KR440_000265"
     CDS             complement(27662..28585)
                     /locus_tag="KR440_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697334.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIIDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(28839..30539)
                     /locus_tag="KR440_000266"
     CDS             complement(28839..30539)
                     /locus_tag="KR440_000266"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBH0697335.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(30950..31363)
                     /locus_tag="KR440_000267"
     CDS             complement(30950..31363)
                     /locus_tag="KR440_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBH0697336.1"
                     /translation="MILVSACLIGINCKYSGDNNENEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKIKSEKDL"
     gene            complement(31431..33809)
                     /locus_tag="KR440_000268"
     CDS             complement(31431..33809)
                     /locus_tag="KR440_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBH0697337.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSEISAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKPGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRNLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            34126..35577
                     /locus_tag="KR440_000269"
     CDS             34126..35577
                     /locus_tag="KR440_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HBH0697338.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(35781..38294)
                     /locus_tag="KR440_000270"
     CDS             complement(35781..38294)
                     /locus_tag="KR440_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBH0697339.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNSYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTSDEQLEVLKSGKADVLLNVSMNPIEGVRTV
                     AKFSSKPFYFITPKGSTDLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETNYIKDTEKLKVGVLLNKPPFQNADKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLEDMAKKHEIDIVAGMIYDYEYSREHNIAMSRPFLSTQYTMMINNKLNENSLNGK
                     RIALAKGIDYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYEAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKSDKESENGNIENERDKIRREYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRITSKIIYNDRKIPMYVIGKIANIDSEKEEKNRLLDKVQR
                     DSLTNIYNAAYIRKLTTQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLVGDKVLIDV
                     AKVLDESFAKDDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVITKRGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(38517..39338)
                     /locus_tag="KR440_000271"
     CDS             complement(38517..39338)
                     /locus_tag="KR440_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH0697340.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSIYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMIVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEMR"
     gene            complement(39857..40333)
                     /locus_tag="KR440_000272"
     CDS             complement(39857..40333)
                     /locus_tag="KR440_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HBH0697341.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVGNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(40367..40945)
                     /locus_tag="KR440_000273"
     CDS             complement(40367..40945)
                     /locus_tag="KR440_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBH0697342.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(41049..43667)
                     /locus_tag="KR440_000274"
     CDS             complement(41049..43667)
                     /locus_tag="KR440_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBH0697343.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIETGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MNRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTVPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMASSISIDSSISIDSSISIDSSISMDLDTSSSLGIPKELMGSNTNNIDTLSNTSK
                     IDTLNKNHYNTAVTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFK
                     KKDLNIDCYLNAFNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGY
                     APMMVTEYCPMGVVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSK
                     VTCMLDNLYELEEIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKKATDLYKK
                     LDEIGTVAGHYYKGVE"
     gene            complement(44159..44842)
                     /locus_tag="KR440_000275"
     CDS             complement(44159..44842)
                     /locus_tag="KR440_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBH0697344.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
BASE COUNT        13587 a         8771 c         4975 g        17650 t
ORIGIN      
        1 actcaaattg tttgatgaag cttttgcagt atttaaagaa tatataatta tttaacatta
       61 aataatccaa tcttgtaata aaatttactc attaaagtat attacagaaa atatctcaaa
      121 ataaactttt tagttcatga tatactcttt tttgcatgaa attagatata tttttatcat
      181 ttcaacaatg aaagggaggc ttacatccat cttgagataa ccccttagat aaaattttta
      241 ataattaaac atcataaaat ataaaaatcg cttatatcaa acgtataagc gattaattat
      301 atttacatat gttttttagc ctctaacaac attttattag cttcttccat atactctata
      361 gccttttcaa tactctttgc tgtttcttct tttccaatag ctctagcctt ttcaacccac
      421 tctctaaatc cttcttcatg agtttcattg tgatttatcc aatgtactaa aagtattttt
      481 aaagtttttt catccttaga ctctgatgat tcctcatgac tatgttcatg gtcgtggtcg
      541 tgagcatggt catgtggatg ttcatgacat atatctccat gagtatgttc gtggcagtgg
      601 tcatggtcat gatgtccatg atgatatctt acaatctctt tatcaaaaat cattagttat
      661 tccccctgtt attttatatt aaactctttt tttataaggt ttacagaatc ttttacaagt
      721 agtgctaatg gtttaccttc taatccaact aactccacat tttctggcat aataggtaaa
      781 agtatcttaa gtgcctcact gtctgcaata gcttcactta tattacaagt aacttctccc
      841 atcatagaat taggtattat tacagaaata ggtgcaacta ttatatttgc ttttttagct
      901 gatactacta tagcattttc ccctgtagct cctttattag catgagcttt catcatagaa
      961 ctagttgcta tagaattagt ccctagtgca taaatttcta catatgtagg taactcttct
     1021 ctcaaagaag aaactatctg ggctccaata cctccaccca ttccatctat cactgctatt
     1081 atcatatgaa attcccctat taattaattt tcttgtatta ttatcttatg gtccatcaat
     1141 cttatttctt taattatccc atctattatc ttttgctctc ctaataaatc cgttaaataa
     1201 atctttccat cacaagggtc tatattaacc acattttcca taaccttttc taactcatta
     1261 ttacttttac aaatgaaagc tgatgattca cacatataaa tctcctccta atatgatttt
     1321 tattaaaaat caaaaaaaga ttcccttagg tatttcaata ctccaaagaa accttcatga
     1381 tttcagtttt tgtatattat tataccttaa gtatatcact ttaatttatg taaagtcaat
     1441 taaagttaac gctttttatt atactcttca ttcttgtatt tttcatatag ttctttaact
     1501 aattgcttct cataatcagt caaatcgccc tccacaaact caacattaaa ttgttcagct
     1561 aatcccttta ctatgttttt ctgtagaata tctatgtcta tttttctgcc aatttcattt
     1621 tctattccac ttgctttttt agctgtaaat ttcatagctc tttctttcaa ttctggtgtc
     1681 ttagttttta ttaatttaaa caatttttct acatcaaaat ccagtattat tgaaccatgt
     1741 tgtaataaaa caccatcttt tctgctttga gcacttccaa ttaccttctt attattaata
     1801 gtaacttcat atgatgcatg tgcattaaaa catgcagctg ataaattttc cctacttatt
     1861 ctctcgcctc tatttaaatt atctgtttct ataccactta gattcaatcc tttaactaga
     1921 ccttcactta tatatctata agataggttg atgctcttat cgattaaagg attatcttct
     1981 cctataatta tactataagt tagctcatta tcatgtaata cagctcttcc acctgtaatt
     2041 cttctagtat agtctatatt catacatcta cacttatcta aatctatctc atcttccaat
     2101 ttctgaaaat atcctatact caaacaagct ggttcccatg tataaaatct aagagttggc
     2161 ttattatgtc ctttcttata tgcagtaaat atagcttcgt caattgccat attcattgct
     2221 ccttcatatg atttattatg tataactctc cactgattca tgattattac gcccccattt
     2281 ttagtataaa tatatcctag aaatcctcta tagatttatt tctcttattg ctaaatctat
     2341 ctcactttga tttaaaggtc ttgaatagtt gtatatagta cttccgggtc tttcattata
     2401 gtatggtcta ttacaatcct tacaacctct tatttcaaaa ggatagcctt tgtttatgtc
     2461 ttttaaaata tcattgtcaa gctttatact atttaagtat ccatttttaa acttaaaata
     2521 ctcttttgga taacctttat ttatcatata agacattaac tgtacccttc tataactttc
     2581 tatgcttggt tgatttatct tttccatttt agttcctcta actggagtaa atgcaaataa
     2641 actaatcgtt acatcatttt cctttaaata aatatataaa ttgtatatat cttcatgact
     2701 ttcacccata ccaactatta tatgtgtact tattttattg ggatatgatt ttgacatttc
     2761 agttataaat tttaactttt catcatagtt atttcctttt attttttcat atagttcttt
     2821 atttgatgca tctattgcta ttcctatttt atcaactcct actgaaaaaa acttctttat
     2881 ctgttcatca ttttctaatt ttgcagatag tgaaattggc atgtctatct taccacttat
     2941 atagtttata aagtctaaaa cagagttatg tgcttcttca cttgccattg attgaataca
     3001 tattctcttt atattttttc ctttataagc ttttaaagca tctaatattt cttcttttga
     3061 aaactctggc caaattactc ttgataattt atcttttcta gtactacttt ctattgattg
     3121 agaacaaaaa ctgcatttat ttatacattt ttctccaatc attatatatg ctgtggtagg
     3181 aggtatatcg ctttttttgt ttaatatccc aagctctatt gctgtcccaa ctgatagtct
     3241 aatcataaaa cgcaacactc cttacactca tttattgtca tattcatttc tatagcctta
     3301 ttctttgcag accttgatgg taatactatt ctattgactc cacacattat agataattgg
     3361 tctagctctt ttctatacac tcctcttggt ctcatacaac ctagattaat ttcagtatct
     3421 ggtaaattaa ttctagcttc acataaaata tctgctaccc cttctaactc aggaggttct
     3481 acattttcat actcagtccc ttttgttgga ataaggacta taaatgtaag cttattagga
     3541 ggatttttct gtaggtattc tataatttca tattcaccct ttatttgtcc tccttttaaa
     3601 ccaatacata tatgtggtgc tacttctgta tgttcttgta ttgtattata aacttcaata
     3661 taatcttctt tactcttttt catcttataa acttctctta ttgtctcatc atcaaacact
     3721 aaatcaaaag aaactatatc taaatactta cacaactcta caatgctatc tttatccata
     3781 agacctaaat gagcattcaa tctatatcct ttctctttta agtttttaat ggtattaata
     3841 tgagtattta tagggacatc tccctcataa ctacaaccgc cactcaatag aaagctagtt
     3901 atattccttg attgtacttt ttcctcatat tcttctattg gcaccatatt gtttaagtag
     3961 tgaccgttgc aatgggcaca tttcaaacta cattgattgc ttgtagtact aagagctagt
     4021 gtctggttag gataagcaaa ctctatttta tttccaaaat tctttaattt aatatcaaat
     4081 gctattttca ttttttcaga tagattctta ttcatagttt attccttctt aagtacattt
     4141 tactcttcta gtaatttctt tgctgttggg aactgctcta tattcgtatg tggtaagaaa
     4201 catgcagaaa taaattcatc catataactt ggatatacac taagttcaac ataagttatt
     4261 tgattaccta tttcttcaag tttatttttt gcatctttac ttatgagagc taagtaagaa
     4321 ccaacaagtg aactattccc tatataagta aatttttctc tatcaatgtc aggaagtaac
     4381 cctataatta ttgagttttc tatgtctagg ttgtttccta taccacctgc tatataaacc
     4441 ctatctaata tactaaaatc catacctaaa ctatctacaa gagtgtatgc acctgaatat
     4501 atagctcctt tagctctgat gaaattatct atatcaactt cattgactac tatatcattt
     4561 tctaaatcaa attcttcttt aaatgctaat acatactctc ctatctcatg ctcattaaat
     4621 ctaactctct tattatctaa atccctgtat attttacctc ttctatctat tacaccttta
     4681 gtaatcattt gacagattaa atcaattata ccagaaccac atataccaac tggtgcacat
     4741 tcatcaataa tctttaatgt tggctctaaa gtttctttat caataattac tttctctatt
     4801 gctcctgctg atgccctcat tccacagctt atccctccac cttcaaaagc aggtcctgct
     4861 gaacatgcac agctcatcat ataatcttta tttccaaata caatctctcc attagttcct
     4921 aaatctataa ataaaacatt ttcttcactt gaccatatcc ctgctgataa tacacctgct
     4981 gttatatctc ctcctacata acttgcaaca gatggagcta agtacacata tgcactgtca
     5041 tttacaaaaa gaccaacatt ttcacccata agttttggag attttaaaaa tggtggtatg
     5101 taaggttctt gtcttaagaa gtctgtatac acacccaaga ataaacttgt catagttgta
     5161 ttcccggcag ccactaatgt aactacatct tccttatcta ttccatttct ttcatatatt
     5221 gtttttaata gtggatttat agtttcctca actatagctt tatgaagtgt ctctaatcca
     5281 tttttcttag ttgaatatat aattctattt ataacatctg ctccatattt tatctgtgca
     5341 tttccagaag atgctttatc tactacctct tttgaatata ggtctactaa acaaacaact
     5401 actgaagttg taccaatatc tattgcaact ccgtataagg aattttcttt attaccttgt
     5461 tctatattta tgattgtaag tttcttttgt ttttgaacat atgttattgt aaccttaaaa
     5521 tcactctttc taaatacagt tggcattttt ctaagtatat ccaatctaaa atctatttca
     5581 ttatatccta ggctatttct aacatatctt tccaatctat ctacatcact tatattatca
     5641 tctaaatttg gttcttccat ttctatgtat ttctttttta tatttgtttt aaattgaagg
     5701 ttatgttctt caattatttt tttagctctt tcaaaaattt ccctatcctc ttttttatta
     5761 ctaccttcta tcttcatacc atgcatagaa gatgatactt tagaaggaac ttcaatttct
     5821 atatctgata ttacctttgt acaacaagct agtatataac cttgctccca ctcatcatct
     5881 gttatatgtc ttgttttttc tgtatcaact tttccattca ataattttac tttacacttc
     5941 ccacaagaca cgtttccatt acaaggagca tctatgaata tatctgcatt tcttgcaacc
     6001 tctagtaata tatcaccctc attacaataa acctctttat tgtttggtgt aaaactcact
     6061 tttatcatgt tatccctccc aatttgttct atatatgata aaaggaaagt atagctaaat
     6121 ttgcgtgcac tcaaatttta ctatactttc cccaattata ataatatctt aatctttaat
     6181 tattctaatc cagcctcgta ttctttatca cttaataacg tatcaagttc agccacatca
     6241 gctagtttta cttttaatat ccagttttca tatgggtctt catttataca ttctggctca
     6301 tcttctaatt tttcattaac ttccaatacc tcaccattaa caggagatat taaatcagaa
     6361 gctactttag aagactcaac tactccaaaa tctactccct tagttacagt atcttctact
     6421 tctggtgttt caacaaacaa tatttctcct aattgatctt gagcatagtc tgttatacct
     6481 atatatacaa catctccatc aattactttt acccactcat gatctttaga gtattttaat
     6541 tctggtaata atttcatttc gatagacccc caatattata ttattgatta aattacttaa
     6601 atcatattaa tattatatca tattatttta aattttccat caattacatt acactttcca
     6661 tagctaatgc tggatgacct ttttcttcta agaaagctaa tactccttca gaatcgtcag
     6721 ctatagtttc atcacaaacc atatcacaga agttttctat tccagtcatt tctttaactg
     6781 tagcatttaa tttatcagct acataatctt ttaattcttt tggcatccaa actattcttt
     6841 ctggtccacc ttctgcataa gcgaattttt tagaagatat gaaatgtctt ccgtgtccca
     6901 taaatcctgg agtttgaact cctccacctg tcatagaagc aagttctccg aaagtcatac
     6961 caactggagt aacacttgca aattctctgt taactacaac aaatccgttt gcttctggca
     7021 ttataccaca gatacattcg aagcatccac aagaagtcat tgggtcttct agtatagaat
     7081 ataatgtaac agattcaact gcaccttgag atatttggtt tacagtttca tttactgaat
     7141 tccatacacc tgttctatca tctaagcact cgcctttttc tattggttga caaggacctg
     7201 ttgggtctaa ttctttagta gccttagcat ctaaccagct aactgctcca caaagaccaa
     7261 gtctttcagg agtaacaaca caaacgtgag ctggtgcaaa tgattgacat aagttacaag
     7321 aatagaaagt atcaacactt tcatcaacta atgaagctaa tctttcatct ctagcattgt
     7381 acttagctat tgcttctcct tttaattcag acactttttc agcatctgta attatagtta
     7441 tttcacactt atcaactact gattcaaatt catctaacat cttagcatat aaaacttctc
     7501 ctatatgctc aagtctaaat cctttatcaa atgcatcttt agagattctt acccaagcca
     7561 tatctctttg tccaacgtgc attacacctt ctatatagtt caagaagtag tggaaacgtc
     7621 tttcaagaac tggctcgaaa tcttcttgca tatttttacc agctatttta actattacag
     7681 caagcggtaa tcttaataca ccatctgcat caacttcatc tatatttggt cctattattt
     7741 ctatcttatg gtcttcaact tctgttgctt ctttcttaac aacaagctcc caagcttcag
     7801 ttctgtttcc accaaactca gcaaacatat cgctctttct aattctctca ccttcaaatg
     7861 ctgctgcaaa tgaaactggg ataggtattt cagtaacttt tatttttatt cctctagctt
     7921 ctaatgaagt agctactatc ttatcgtaat ctttttgagt taataggttc attggaactt
     7981 caagaactgt ttggtcagtt ataactggga atcctaaagc tattgctcca gcacctgcag
     8041 atacaactaa ttcacttaat ggtccaaatg cattaacaaa tgcaggaact ctattagctg
     8101 tgtactctaa taatccgttt aaatcaccag gagttaatcc accgaatatt aatgctgctc
     8161 ttactgcaac agatacaacg tgtataacag aagttacatc atatcctaga ggtataactc
     8221 ttagctcaag acccattttt actccagctt ctatagcttg gtctataact ttaccaacta
     8281 agaaagttag taaaccttta gattggtaat cttttataac ttttgctgct gattctgcat
     8341 ctggacactc accaagaaca actgcaacac caggtatatc tcctgtaact aatggtacac
     8401 caagtgatct gattataggg tcagttatat gacctgcaca tggctcacta tatggagcat
     8461 ccatagttga atattttaat gcttcaatta cttctgcagc taaagccgta gctaaaccag
     8521 cattaaaagc atgttctagt aaatgagttc tatttattaa agattttact acttctaaag
     8581 caccttctaa gtcccctaaa gtgttcatct tttgtcctgt tgcagcatat atacaaggta
     8641 atgaatatgc tgtatcaggg aaagcaactt tatgctcttt tccattcttt tctatagctt
     8701 cagctaacat agcttgagcc gcacctaaag cttgttctga ccctgtaaag attatattat
     8761 atagattcat tctatttccc cctttaatat ttacacctat tttttattaa ctatacccta
     8821 aaaaccctag aaagcaaatc caccttcatt taactctaag aaagagtctg catataaaga
     8881 tgccccgttg attatgtcac aagattttac agttttcatt aattctgggt cacatggatt
     8941 cattatagct gaactaaatc cattagccat tgccattgct aagaatgcac tatctaaaac
     9001 aggtctaaca tgcttaggac atccattaga tacgttagat aatcctcctg tagttaaaag
     9061 tcccatctca gtcatcattt ttatagcttc taaaacttct acttgttttt cttgcatacc
     9121 ttttataact aagcataatg ggtcaaatag tatatcagtt ggctccattc ctaacattaa
     9181 gcctttttct aatatttctt ggcagtaagc catacgctca tcattatctc ttgggatacc
     9241 ttcttttgca cataaaccta taaccattgc ttcgtattca gctgctatat ctattaaatc
     9301 aaatcttgag ccagcatcag cagagtttat tattggtttt gcattagttc tatcataaac
     9361 tttaagtcca gcttcgatag cttttttatt agctgtatct aatgctatag gaacattgtt
     9421 gaactcagat tgaagtaatt tgattcccca tgtcattatt tcttcgccat ctctttcggc
     9481 aggtcctata ttaaaatcta tatagtgtgc tcctgcttct aattgctctt ttgctctttt
     9541 taagattgga gctgggtctc tttctgctaa tgcttttctt attgaaggtg atatacaatg
     9601 tattctctca cctataatca taaatttttc catttataat gtcccccttt aaatttaata
     9661 ttaatagtta gttaatattt tattagtttg tttggcatgc tttttcttgg tatcctttta
     9721 agaacttagg taattccata gattcctctg gtcctataac tacattccat cctggtaagt
     9781 tatcttctat atcaccttta agtactgcaa cttttcctgg aatgataagg tctttacaat
     9841 tagttacttc ttctacctta ctttctttaa tgaaagcaga gattgagttt ccaccaaatt
     9901 taccagcagc ccaagatgta agaactgaat atccacctgc atctggtatt actaaccata
     9961 ctggaacttt tgatctttct atatcaccag caactatgaa gtaagttaat gcaaagtcaa
    10021 cagtaactaa tactggagaa ttttcatctg ggttgttgat tggataaatc tttggttcaa
    10081 ctctcattgg tctttgtggg tcagtgtata tgttttgtct tagtgcaaat aatggtaatg
    10141 ccttagcata actaatatca tctattacta ttatagaacc gtattttata gtgaatattg
    10201 atgataaagc aacttccatc atagggtttg aattagataa tctatttgca aatactattg
    10261 aaggatatcc aaatgttcta tcttgctctt ttaaagctgt tcttctaact tgtatagcat
    10321 tagtgtaagt atcttttatg ttttctcctg ttacatctaa tactaattct ttatatccag
    10381 cggcttgaat taattcaact gtttcatata attcttctaa gctaccagct tttacaccta
    10441 atggtaaact tgctccttta actacttcta tcatatcttt gtagttctct ttagttgctc
    10501 catataccat tggtctagca tcttttaata cttcaacagc ttctttaact acttgtgcat
    10561 catcacaagc taaaatataa gctacttcta atccactgcc ttttatttta tctattaatt
    10621 ttaaatacgc atctttatct ccacaatact ctaaaacagc catttcaact ttcatttgtt
    10681 caccaattct tacatagtca acttttttca tgttagctat tttagcatct actgcttcat
    10741 cactcataca agtacagaat gaaactgcat atctatttct acttactaat gttttttcat
    10801 gtctaaataa tactgtttct ccacctagtt catactcaga tgcaccagca ccaactttta
    10861 aagctttcat taaaggtgca gtagcctctg ataattttgc tatagcgtca tcagacatat
    10921 gtgggcattt acctacttct acagctcctg aagcaacctt catagagaaa gccatacaag
    10981 ttgggaatcc acaatcctta cagtttttct ttggtgttaa tttaaatata tctaaagctt
    11041 ttaatgccat tattttttcc ccctaacctt aacaatttaa ttaagctaat gcgttaacca
    11101 attcttttat agtttctatt gattttggat gacgaagtat aactgcgttt gaaccaccta
    11161 ctaaaacact tgctgcagtt gaaacttcca ttgctatagt tctttcttct ggacaacccc
    11221 aatctggctc atcttctatt ggagctatag cttctttaac atgacctact tcaaaagcta
    11281 ctggtgttat gataggcatt tgtaatgtct tatcattttg accaaatgct gcaagtctaa
    11341 ttctatccat agtagatgca acgtactcat atccataacc aactgctgaa catccaacat
    11401 tcataactat attttcacct ttaacgccta gttgagttaa caatacgttt aattgtttag
    11461 ctaggtttat atcaacagaa gattcagctc ctactttatg tgcataagcc ataccagctg
    11521 atgctcctac tcctttgtag ttatcttcta ctgctgacat aaataagcag ttatgtccat
    11581 ccaatgtttg agctaatttt tcaaataatt taccatcttt ttcatgattt cctgaaccag
    11641 ctacaactaa aggtaatttt attgcttcaa ttactgcctt agctacatca gcacattcat
    11701 caactgactt atctaatcca tttgggtcag aaccatcaaa ttttaaacaa ataaaatctg
    11761 cttgtgtatt agcttcaaca tatttagccc actctactgg acaattagct acatctttgt
    11821 ataattcctt atatgaatca gtccagcttt caggataaac gtctgatatt tgtataccta
    11881 tttttggaga attacctact tctccatcaa aactataaaa aggtaataca ttttccccac
    11941 ctaatttaat tgctttttcc cctattccta cttcaacttc tgatattttt ccagaatatt
    12001 tttgagtaga cattttaaat gccatatttt tttccccctt aagcctattt taattctaat
    12061 ttagcaaaaa tttctttcat agccacttta gattttgaat cttcaggtaa attaactaaa
    12121 ggaatacctg tagaatcata ttcatatatc aattcatcca taggtacaac accgattagc
    12181 tctaggttat gcttttcaat ttcttcttta acaccatcat tcataacccc atttggaact
    12241 ttatttacaa tcaaaagtat tcttccaaca cttaacttta gttcctcagc taaatctctt
    12301 attctagcaa cagcttgtat gctacgtctt gagcaatcac taaccaataa aagtgtatca
    12361 acatgtctag tgacttttct acttagatgt tccataccag cttcgttgtc catgactaag
    12421 tatttgtaat gaccagatat tttgtttact tgttctctaa gtatcccatt tacaaaacag
    12481 taacatcctt caccttctga cctacccatt actaataagt catatccttc gccttcttcg
    12541 attatagaat ttaatctaaa ttgaagatat tgtgcctttg tcataccacc agggaatgca
    12601 ttgcctaact tttctctttg atttacttct tctcttattt ctcctattgt tgcttcaact
    12661 tcgataccta acacttcatt tatgttagca ttggcatcag catctacaac taatactggt
    12721 ccctttttgt ccttaaccaa ataatcaatt aaaagccctg tgagacttgt tttaccagtt
    12781 ccaccttttc ctgcaacagc tatattgtat cccattttaa gacttccccc ttaaatctag
    12841 ccacagagcc ttatgtactc tgtctgtcta ttataattta ggtacagaat gaacacattc
    12901 tccatgtaca tcatgaagtg cttccattag cccttcagat aaacttggat gtggatgtat
    12961 tgcatcccct acttgttcta ctgttaatcc tagatgaaca gcaagtgaca actctgtcaa
    13021 taaatctgta gcatgaggtc ctactactgc tgcacctatt atcttatcag tttctttgtc
    13081 agctataact tttacaaatc cttggaaatg tcctatagct tgtgcttttc caagacctct
    13141 aaaatcaaat tgacctacat tgtattctac accttcagct tcaagttgct tttcagtctt
    13201 accaacacca gcaacttcag gttcagtata aacacatctt ggaattgctc tataatctac
    13261 taccttagtc ttacctaaag cattttcaac tgctacgata ccttctttag aagcaacatg
    13321 tgctaggaaa ggagtatcta ttatatcgcc tattgcgtat ataccttcta cattagtttc
    13381 aagatgttca ttaactacta cttttcctct ttccatttca attcctatat cttctactcc
    13441 agaattgtca aggttaggtc ttctaccaac acatactaat gcatattgtg cttctataac
    13501 ctttccatta gaaagagttg caaccgcctt accatcaact acttcacaag tttgtactcc
    13561 aatacctgta ataaccttaa ttttatcttt cttaaattgt ctaagtagtt gtttagctac
    13621 atctttgtct tcatttaata gtatttggtc aaccatctca actatagtta cttctgttcc
    13681 taaggctcta aagaactgcc ctatctcaca tcctataact ccaccaccta ctattaacat
    13741 agactcaggt atctcttcaa gtccaagaac ttcatcacta gttattacaa cttttccatc
    13801 atatgggaac attcttggta caactggaac agaaccattt gctagtatta tcttatctgc
    13861 ttttatagtt tctactgttc catcatcttt agttacttct atagtgtttt tatcaattaa
    13921 ttttccaaaa ccattaacta agtttactcc tcttttttca aataagaact ctataccact
    13981 tattaattgg tttacaactt tattttttct ttccattatg gcagtgaagt ttggcttaac
    14041 tgtaccatct atttctatac caaaatcctt tgcttctttt acagtattca atactcctga
    14101 agaagcaaga agtgctttag ttggtataca tcctgcattt aagcaagttc ctccaactct
    14161 tcttttttca ataaccgtta catctgcacc aagcatagaa gcttttatag ctgctacata
    14221 tcctcctggt ccaccaccta ctactactat cttcatcctt aattccccca aacctaatat
    14281 aatctacaat cttgcaatac tataaacctg cggcatccag tatagcaggt atattctctt
    14341 ctgccccaat tgccattata tgaacaccat cacataaatc ttcttctttt aattgtttga
    14401 taagttcccc tgccattttt attccttcac ttacccagtt ttcttttcca gctgctctta
    14461 atctttcgat ttgttcatct ggaacgaata ttcctggtac atttgcagtc ataaatttag
    14521 ccattcctgg tgattttaaa ggtactattc ctgctaatac cttgcaatcc atatcccttg
    14581 ttaattctct aaactttctc attgtattta tgtcataaag tgcttgtgtt tggaaaaatt
    14641 tagcccctgc ttttatcttc ttttgcattt ttaataattg aacttctatt ggagaatatt
    14701 ctggagttac tgatgctccc agataaaaat ctggagaacc ttttaactta ttaccagcca
    14761 tgtccgtacc tgccattaaa gtctcagcag tttgaagtat ccccacagaa tctaaatcaa
    14821 acactccctt agcctgtgga tggtctccta cgctagtatg gtctcctgtt agtgccaaca
    14881 aattgtttat cccaaataca cctgctgaca acatttctcc ttgtatagct attctatttc
    14941 tatctctccc tgttatttga ataactggct ctaatccagc atcttttaat aatttacatg
    15001 tagcaagaga agttgctctc ataactgccg attgaaagtc tgttacattt gctgcatgaa
    15061 ctcttccaac taacggcttt gcacattcta ttaaatgtga aaggtcagtc ccttttggag
    15121 gagccatttc agtagtaact gcaaattttc cactttctaa tgtttctctt aataagctca
    15181 tctctaatcc cccttttaac cttctaaaaa aaacttttat gaatttgctg ctgcttcttt
    15241 ctttttcttt gcacttaaac ttcttggatt attttgctta gaatagtctt ttggtggtct
    15301 tatttctgct aagttatcta attgtcctat agcttctagt ctttcatata tctttatcca
    15361 agcacaatca ttctctgagt ttacttcaca cttaccattt ttagctccac cacaagcccc
    15421 attcataaga cctttagcac acatagttac tggacatatc ccacctgtcc aaccaagttc
    15481 acagtctcca caagccttac aggcttcttc atattctcca actctttgta cttcccctat
    15541 aaacaaagta ttgttggctg gataaactgg cttatctttt aaattcttaa ctattgtctg
    15601 tgtaccatct ccacatgcta aagataaaac tgcatcagct tccttagtct cttcttttag
    15661 agcctttaaa tcctttttag actttaaaag gttacaagca gggtcaagca ttacatatcc
    15721 taagattttt ttgccttcac cttcaagagt ttccttcatt tttaaaactt cttcttcccc
    15781 accactttta caagttgctg cacattgatt acaaccaact aaaaccagct tgtcaaaatc
    15841 ctttaagtac cctagcactt cttctaatgg tttattttca gaaattatca tatctacttc
    15901 ccccctaaac cttccacata tatatctcta tttattgttt tgtaatttac aagccttaac
    15961 tacatgtttt gcaagtatag atgttgtaac tgaaccaacc cccgctggaa ctggagttat
    16021 cattgatact ttgtctaaaa ctgcatttgt atcaacatct ccacataaat ttccttcttc
    16081 atcaacattt ataccaacgt ctataactac cgctccatct ttaacaaaac tttcatcaac
    16141 catcttagct ctaccaatag ctgctattag cacgtctgcc tctgctgcta cacctgatag
    16201 atttttagtt tttgaatgac atattgtaac agttgcatgt tcacttaata aaagcattga
    16261 tactggcttt ccaactacca ttgatcttcc aagaactgtt accttacttc cttttaaatc
    16321 aacattatag tgttttaata tctcaactac tgctgtaggt gtacatggtg gaaatccact
    16381 tttatcccct tccattacct tagcagagtt tattggacta aaacaatcta cgtctttttc
    16441 cggtgcaata acatatttta taacttcttc atttaagtgt tttggaagag gtctaaaaca
    16501 taatatacca ttaacatttt tgtcatcatt aactctcttt aatacatcaa tatattcctc
    16561 ttgtgttata tcctcagcta actctaacac ttctgttgtt atacctatgt tttgacatct
    16621 ctttagtgct cctctttcat aagataaatc atcacttctt gccccaactc taactattgt
    16681 tagtttagga tttatacctt ctttaactag taaatcgact tcttttatta attcttcact
    16741 aattttatca gctacaggtt ttccttttat aatttgtcct ttagttgaca ttccttccat
    16801 acattcacaa ctcccctaca agcaaactta aattaattta ttctatatac cacatattaa
    16861 taacatagta tctactttcg tcatttaaaa attattttcc aagagctttt tcaactttgg
    16921 cataaacttc atcacaaatt tttacaccat cttcaactaa cttatctact tctattttta
    16981 ccttattaac atattcttta tcttgtatag aatttatatt tataactaca tttaattgtc
    17041 caccaagtat agcagctctt aaacactgaa ctcctacacc aacatcactt atagcaagtc
    17101 ttgaaccttt atcaactaaa tcttcatgta acttaatagc ttcatagcaa actcttacta
    17161 tgtttaatgg tacttcacaa gcaactttta atgccttttc cattgtttct gcttttattc
    17221 ttttttcttc ttctgtttct ttaggaagcc cgtaagcttt tgataatggt aagaaacatt
    17281 ctgcatcatc atctatcatt tttaataaat ctttttctaa ctttcctgcc ttacttaata
    17341 tctcttttac gctttcttca tactcagcat acttcttctt ccctattgta aggttacata
    17401 ccatactacc taaagccata cctattgcac ctactagtgc agcagctccc ccaccacctg
    17461 gtactgcttc tttggaagat agtacttcta caaaatcaac acaagtttta tctgctattt
    17521 tcatgataat cctccttgaa aggatgtagg aaaatatctc aaattattcg agatatttta
    17581 cgaactattt taagatatct cctactcttt atttataaat taatttcctt agaataatcc
    17641 agctattact ccattttcat caacatctat tttttctgct gctggaactt ttggtaatcc
    17701 tggcatcttc attatttctc cagtaagtgc aactataaat ccagctcctg ctgaaattgt
    17761 tgcttgtctt acagttattc taaatcctgt tggtctacct aatttagttt ggtcatcagt
    17821 taaagaatat tgagtcttag ccatacatac tggaacttta gtaaatccaa gtttttctaa
    17881 gttagctatt tccttatcag cttcaggagt ataatctaca ccatctgctc cataaatctt
    17941 agtagctata gcatttatct tgtcttttat agataaatca tcttcatagc agaatctaaa
    18001 gttattttct tcttcatcca ataatcttaa aacttcttta gctactgcaa ttccaccttc
    18061 tccacctttt gcccatactt cagaaagagc aacattaaca cctaattcct tacacttgtc
    18121 ttcaacaagt ttaagttcag cttcagtatc aagtgggaat ctattgattg caacaactgc
    18181 tggtaatcca tatacttgag ttatgttttc aacatgtttt aataagtttg gaagtccttt
    18241 ttcaagagct tctaggtttt cgttatttaa gtctgcttta gcaactccac cattgtattt
    18301 taatgctctt acagtagcaa ctattataac agcatctggt tttaagtttg ccattctaca
    18361 ttttatatct aagaattttt ctgctccaag gtcagcaccg aaaccagcct cagttattac
    18421 atagtctgca aagtgcatag ccattcttgt agctataaca gagttacatc catgagctat
    18481 atttgcgaat ggtccaccgt gtacaaatga tggtgttcct tcaagagttt gaactaggtt
    18541 tggttttaaa gcatctttta ataatgcagc catagcacca ttagctttta attgtctagc
    18601 tgttacaggt tcaccttcat agctgtatcc aactattatg tttcctaatc tttcttttaa
    18661 atcagatata tcacttgcca aacagaacgc tgccattatt tcagaagcaa cagttatatc
    18721 gaatccatct tgtctaactg ctccatcacc ttttttaccc ataccatcaa cgatatttct
    18781 aagttgtcta tcgttcatgt ccacacatct tctccaagtt attttcttag ggtctattct
    18841 aagtgcattt ccttggtgaa tatggttatc aagcatagca gctaaaagat tgttagcagc
    18901 tcctatagcg tgaaaatctc ctgtgaagtg taagtttata tcttccatag gaacaacttg
    18961 tgcatatcct ccaccagctg ctccaccttt aacaccaaat actggtccta gagatggttc
    19021 tcttaaagca actaatacat ttttgtctag tttagcaaat gcatcagcaa cacctatagt
    19081 agtagtagtt tttccttcac ctgctggagt tggatttata gctgtagtta gtattaactt
    19141 agctttttta ccagtctctc tttttagtag attgtagtca acttttgctt tgtatttacc
    19201 ataaagttct acatcatctt cacctagtcc taattttttt gcaacctctc taatatcttg
    19261 aggcttagct tcttgagcta tttcaatgtc agatttaaat cccataactg aatctcctcc
    19321 cacaacctaa atagatttaa aatataattt ttgtaaaaat tgtattaata tgtaaaaatt
    19381 atattttata agataattat acatgtaaaa ttacttaaaa actagctact aagctattaa
    19441 aatattcaaa cttaatagct agttatttat ctaattactt acttattacc tatctattgc
    19501 ctatgtctaa cctttaacca acttaaaatc ccaaatcaaa atttctatct attttattta
    19561 tccttaagtg acattaattt atcttttatt gctttaatct gttctttcgt tgtttcacta
    19621 gaatcatatg gagattgatt agctctatca gaatcaataa catcttggtt atagtaaata
    19681 aatcctaaag attctccatc ttctaagttt tggattataa attcttcatc ttccttattt
    19741 ctaattttat ttcctactac aaatacttta tttacaccta tatcatgtcc aagtttctta
    19801 acttttctat aagtctgtag acttctctct cctggttcta caactactat aaaagcatct
    19861 acaccttggg ctgttcctct tcctaagtgt tcaattcccg cttccatatc cataaccact
    19921 acatctttat tttgtagtat taaatgtgag cagagtctct tcaaaagaac atgctctgga
    19981 catacacatc cagtaccacc agaatctacg gttcccaatg taagtaatct tactccattg
    20041 tattctttac agaaattttc agggatatcg tctactttag gattcatctt aaacattttt
    20101 ccaaatgaac ctacagaagc agctgttcta tctgaaacta attttttcat ttctgatata
    20161 ggtactattg attcatatac ttcttttgga aaacctagtg ctaaagctaa attagcatca
    20221 gggtcggcat caacagcaac aactctgtat ccgtcttcag caaacatcct agagagcatt
    20281 gaagaaaaag tcgttttacc tactccacct ttacctgtta ttgctatctt cataatccct
    20341 accacctttt attactattt gctttttagt agttaatttt acagttaaat attttttata
    20401 ctctatattt ttatacacta aaaataaact acagctagtc cttacaggaa ccaattaaat
    20461 ttaccctact agcttttcaa agcagcatgt atagttttat tattctttaa tattgtttat
    20521 attttattta ttcagtatat tcattatatt tcgatgttct ttactttaat atttgacttt
    20581 attcaacttt actcaaccag aaacttctta taaaaacgtt tattctataa cttcactatt
    20641 ttttaagctt cagccttttc tgccattttt tcttcaacta atgcttcaaa gtgaacacgt
    20701 tttttctcta tatcttctat tattgtagct gctaactctt ttggatttgt atttacagta
    20761 aatgtagctc caactttatc ttttaactca ctagttaata tatccactgc tcttgaagaa
    20821 ccagctattg gaggcattat tcctaaataa gtgtctatac cagaagctac aacatagcat
    20881 cctatagcaa ctgctttttc tgacatccat tctggagcta tacctacaac tggaaggtca
    20941 cacatatcca tatctaagta attagctgct gctccaacta aatctagtat acgacttata
    21001 tcaacacaag aacccatatg tagtactggt gggattccta ctaatttaca gacagttgca
    21061 agacctttac ctgcatattt ttcagccgca tcagattcca ttaaaccaaa cttagctgct
    21121 gctgaagctc cacaacctgt agtaactact attatgtcgt ttttgattaa ttctttcatt
    21181 attgttacgt gagcctcatt tgaaactcct ttagcattgt tacatccaac tacaccagct
    21241 gctcctctta atactccagc ttttagacag tctgttaatg gcttaactgt tcctgctggg
    21301 tctatatgag agtttacaac tctgtctaat tgatttacta ctgcttctac actataccca
    21361 actgttgcgc ctgattttaa ttcaggtata agtacttttg atttatctct atttttaaag
    21421 tttaaaatag cttctttaac tattgcttta gcatcatcaa gtgcttgttc ttctctaaat
    21481 tcaatataag ttgaacctgt tatcttagct tttggagaag ttgttacaaa tttagtatgg
    21541 taacaatctg caactctagc taatgcaggg aatatacatt gaacgtcaac tattacagct
    21601 tcaactgctc cagttattat tgcaagttct tgttgatgga agtctcctgc tatttttaca
    21661 ccatgtctca ttgtaatctc attacctgta caacacatac ctgctaagtt tattccatca
    21721 gcaccaactt ctttagctaa agctactaag tctggctctt ctgatgctaa aactatcatt
    21781 tctgataatg ctggctcatg tccatgtaat attatgttaa ctttattttc ttctaatact
    21841 gctaaattag cctctgttct tcttggcact ggagttccaa ataagatatc gcttagacga
    21901 gtacctatca tagaaccacc ccaaccatca gccatagaag ttctaagact catgtgtatt
    21961 aaactatcta tatcaccagt acatcctata tgagtagagt gcattattgt tgcaatttct
    22021 ctatctatag ctcttggctc tatagcgtat tctttccata ttttttgtct tggttctggt
    22081 gcatttttta taaatcttaa tgtcccaaaa ggttttccaa attccattaa tgcaatttct
    22141 gccacttcat gtgctacatc atagatatct ctaccttctg tctctacatc ccattcttta
    22201 gctaaagtaa ttaacttagc ttcgtctctt attttatagt ttccatttgg atcagctaat
    22261 gctaatgtat gtgctatatc tctagcatgg tctgaatggg cagctgtccc accagcaacc
    22321 attcttgcaa agtttctacc tactatagta tgctcatctg ctccacataa cccccttgga
    22381 gtcttagggc ttattctaca tggtcccatt ccacatattc tacagcaaac accttgaaga
    22441 ccaaaaccac attgtgcctt aaagccttct tttctatcat acattgtttc tacgccttct
    22501 tctctagctt tagctatcat cttctggctg tttaaatcaa ttgttaacat tttttcatcc
    22561 atattcctat cccctttttt tgatttttaa aatcccccag gtgactgact tcatgtcata
    22621 aaataataaa ataaatgctt tctaagttga ttatatacta attttcagtg ctttgcttta
    22681 gatttaaata tttttttaac aaaattagat aatacataaa aattttattt tctacactta
    22741 atttaagttg attttatcgc ttgtttttgg ctatttgtta atatctaaac ctactttttt
    22801 gatacttcag tttaattaaa ttatgttttg atttttttaa atcaaatttg aaatttttct
    22861 agtcattttt atattattgc agattgtttg tgtatttatt atctttcact actgtttgga
    22921 cttattacag atttttactc cctaaactta attagtaaaa acttcttaga atagcatttt
    22981 ttggactaat ttttttgaat ttttttattt ttcctacttt aatattaatt atagcttcaa
    23041 attgaacatt tgtatatatt catttgaagc tataattatt ttttatataa aactttttta
    23101 atacagcatt aatttagaca ttttgactat tttttctact ttaattcatt tatttcaagc
    23161 gatttatcct atttcatatt tttagctaca atagccccat atgcaataga atataattta
    23221 gattcatgtg aatcagctct tgacactaaa actataggag cttttgcacc cataattata
    23281 cctgctgatt tagagtctgc catatatgta aaggctttcc ctattccatt gccaacctct
    23341 attgttggaa ccaatatgat atctacatct ccagatactt cacttttaaa cccttttatc
    23401 tcactagctt cttttgaaac agctaaatca aaagccagag gaccttctac aattacattt
    23461 tcaccaaatt ctccttcttt acaagctttt gctaacatat ctgcatctac agttgcttgc
    23521 atcttaggat ttactttctc tttagctgcc aaacatgcaa cttttacagt ttccattcct
    23581 aaagcttttg cagcttttat agaatttttt aatatctttg ccttttgttc ataatcagga
    23641 gctatattca tacctccatc tgtagttata agtaatttat ggtatttttc taactcatat
    23701 accataacat gacttaataa actatcagtt ctaagaccat agtctttatt taacacttct
    23761 tttaatatga cagatgtatc taataatcct ttcattacaa agtcggcttc tttacttgat
    23821 actaatttaa ctgctatttc tgcacactct ttgatatcat ccttatccac tattttgata
    23881 ccacttaaat caaatcctat ttcttttgat atttctctta ttttgccttc atttccaacc
    23941 aatataggag taattatatt tctttctaca gcatccttta ttgccattaa aacttcttta
    24001 tcatgagctg ctgctacaga gagtacaatt ttctctcctc ccttaagttg ttctaaaata
    24061 tcatctaatt tttttatcaa aagtaacccc tcccttatat atatttaact aaataatacc
    24121 atatttgcta taccttttct caatttattt tttatgttat aaagaattta tatgactaaa
    24181 agacagttta atattttatt ctgggatttc atcaatagct cttaaaagct ttgcaggaac
    24241 accacctaca atagtatttt ttgttacaac tgctccagca gcgataattg cattatctcc
    24301 aatagtaact cctggaactc ttcatacaaa aaagaggctt atctctattt tgagatagcc
    24361 ccacttaacc gataaaatta aatacaacct aacaatatcc ttacttattt attccaagtt
    24421 ctcttagaca agcaatagta atttcatctt gtggatttaa ttcataagct ctttctatat
    24481 agtaagttgc atcatcaata tcaccattat ttaaataagc cattcctaaa ttgcaaagta
    24541 tttctgggtc ttcctttatc ttagccgctt gttcaaaata ctctattgct ttatctaaat
    24601 tattcttata agcctcacaa agccccaatt ccactatagt atccacctga ttaggcttta
    24661 ttatcaatat cttctcaaga tacatcttag cctgctctat ttcgcccata cccttatatc
    24721 caagtgctat cataaacagc aagttccacc aatccatatg ttcttcttct aatggaagta
    24781 acttctctaa tccttcctta aacttacctt ggaatacaag tgtatatcct tcttcatatt
    24841 gaactttaaa gtccatcttg cctagattct cttgtacctc agctactaaa tcagcatcta
    24901 accctagcct taatgcttct tcccaagtca gcttagtctt taaatattga ccttgattat
    24961 aataatggta gcctaagtga taataaccta atgcaaaatt ctcatcaaca tcaattacct
    25021 tttcaagctt agccatggct tcaagtaaga atgcattcat tgccttatca tccatatctt
    25081 tttgatactg tttagctatc tcttggcaaa caatcgcata gttatacatt gcatctaaat
    25141 catctggata cattgttata aatgctttta tatatattaa agaatctgta tgttcaccaa
    25201 tctcaaagta ttttctcgac atatatccca tatacgattt caaatccaaa tcaatatctt
    25261 ttgataaagc atttaaaaac ttctcatact ctgcattgtt tttaaactta ctatctattc
    25321 caattatata tatcattgca tctgctatag acattgaatt taacttatct tgagcagtct
    25381 tttcttttat cccttttact aatacctcat tttttatagg tacatctaat ccgccttttg
    25441 gcactttata gctttttaat ttaaactctc caccatgttt tatagttata aaagctaatt
    25501 cttctgcttt ttttagcaaa tatttttcta tcttaaaatc cattttatcc tcctaaatac
    25561 ttaaatctct actttatgag ttttagattt ctcaaatatt tttttatcat catactcaaa
    25621 taagactaca ttttctactt ctcttatttc tgtatttata tcatttttta aatccacaac
    25681 attaaatcta aatggctcaa aatgaacata ttttattata tgtttagctg gtttatcaac
    25741 aaaactaggt aaatatgttg ataagttatc attatcttgt aaaaatacat ggcatctatt
    25801 cttaaaatca tccacatcta gcttattaaa aaactgtggt atatttgatg ttttacccag
    25861 tgatatatag tcatatttta atatatcatt gaatacatca ttatctatgt tgattttttc
    25921 attatagaaa tccatcaata ttttatataa ctgattcttt ccttgtgcca aatcaaagta
    25981 gccattttca tcaaaatact tggcaaaaac ctcaaagaat ttaaaaggac tttgtttata
    26041 aaatctgcct ataatatatc tcatagataa cacaaagttt ttagaattgt agtatctctc
    26101 cagtatatct tctatatcct ttagcttcaa agtttcttca tagcttatat aattattgta
    26161 taaaaactca tatggagcat aatccttata tttaaaacca tgctcatctg caacttttct
    26221 catacctgtc cctttaatca tctttaaaaa gcctaattgc agatgctcta tacctagatt
    26281 aaatacatca ttaaaagaat tttcaaagct cttataatct tcataaggca gtcctgcaat
    26341 taagtctaag tgctgatgta tatttctata agatgctaca gtttgtacaa catgagacaa
    26401 ctttttaaaa tcatcccttc tacctactgc atcaagtgtt ttttcattcg ttgactgaac
    26461 ccctatttca aattgaaaaa gaccttcttt acaatcagcc aaaaactcaa gcatcttatc
    26521 atctatcaaa tacgctgtaa cttcaaaatg ataagttgta tatccattat catttttcat
    26581 aaggaaatcc atgatttcca tagccacttt tctatttgca ttgaatgtcc tatctataaa
    26641 ctttatctgt gatactcttg catctattaa agcttttaaa tcactcttta tcctgtctaa
    26701 actaaaatac cttagacctg gaatagtaga tgataaacag tactggcagt taaaaggaca
    26761 acctcttgat gattcataat acactattct attttcatat tcttttggat tcaagttttc
    26821 ataaggacta ggaatctcat ccaaatcttg aagtaattcc ataggcttat tcactattat
    26881 ctcattgcct tgtctatata caagtccatt tacatcattt attttcatct ttccttgcaa
    26941 gtgaagtact aagtctctaa aaattaattc tccctctcca taaagtatat aatccacaaa
    27001 atcatacttt ttcattgcct cataactatc ataactcact tccggtccgc ccaaagctat
    27061 tttagtatta ggtctaattt tttttagatt atcacaaagt ttaactatat ctcccacatt
    27121 ccagatatat gttgagaata atattatatc atattcattt ttatatatat cctttaaaat
    27181 atagtccaaa tcgttgttta tcgtgtattc tttcatatta acttctatca aatctcttac
    27241 aaattctttt agatatctta tagccaaatt tgtatgaata aacttggaat ttaatgttgt
    27301 taataaaatt ttcaaatatt tctcacctct ttttaagatt atactgtctt tttgtacccc
    27361 ttataggtta aaatagtata tgtaaagtct aaataatatc tatataatta atttattaat
    27421 ttcaagtaat gtgaggaaag gaagtttttt atggaattaa ttgaaaaagt atctatcaac
    27481 tctttatcaa aaagagattt actacttatt ataaagtctc ttgaatacac taatgaaaat
    27541 actcaaatta gtgatttcat tgaccttagg aataatatag taaaggaatt atgtttttta
    27601 actgacacta aggaaaaaga ttttttagat tatctagaaa aaaatagcac actctaataa
    27661 cctaaacctt tttaaacttt aatatataaa tacctgattt agaatttatt tttatgctta
    27721 atatattatc attaaaggtt ttcgacataa ctgttatttg attaaatata gggtctatta
    27781 tagtattacc ttcttcaagt cttataagac ttcttgggtc tatgctttta ttatatgcct
    27841 taactccaat tttatatttt ctcaatttaa caacatattc ggacatagaa aaaataaaat
    27901 ctatattatc ttcatttaat ttactatctt cacccaaaat ggataatata gattttttta
    27961 tgctattaat tccttcattt tctacagttc tatcttccat atatacatca tatagaacat
    28021 ttaaaacatt taataaaata ctttcttcat cataatcttc agatttcaaa tcaatgtact
    28081 tatcaattaa ttgtattgct ttaatccttg ccatctgaaa ctttacaaca tctgatttgt
    28141 ctaattctat agaaaatcct attatcttgt ctttaatact ctgtagtaat tcgacatact
    28201 ctattttact attatttata agattgtgca taatggaatt atacactaca gaacctaata
    28261 aataatcaaa taaaagttct tcttttttaa aataaataac atactttttt attagtttaa
    28321 taacctcatc ttcgcttttt tgaatattag tatttgataa aattagtggt agtaaaatat
    28381 ttaaaaaatt gatatttcct attttttctc cataaaatct atagtaaata agttcatctc
    28441 tcagtctatt ttcgtctata tacacacatc tttgcgtttt atcatctatt aaggattttg
    28501 tatttattat agttaaatct ttaagagccc tacttgcact actaaaagaa gatgtgtaga
    28561 ctttttttag taattcgtca ttcataaata tatcctttct gaaaaattga atgtaaataa
    28621 attgttatta aaaaaaaaga gacagcccgt ttaagaagct gtcatatata gggggatagt
    28681 agtgtacaaa aatcattgta atctactatc tatatattat tatacgatac tactttttta
    28741 tatgtaaagc atcagttttc gacatcccta tattttacag ttatacaaat tgcgtactaa
    28801 gttcaatttt attcttttta tttcaacatt ttatcatgct acattctttc cggagcgtgc
    28861 attcccaaca atttcagaga attttctaaa gtttgttttg ttgctaaaac taatgcaagt
    28921 cttgcttttc ttatttccaa attttctact aaaattggat tgtcatgata aaatttatta
    28981 aacgcttgag ctaaatctaa cataaatcta gtcactatat gtggctcatt tttctttaga
    29041 gcaagtaaaa tattcttatt gaatgattct ataactctaa gaacttctgc actatcttca
    29101 tcagctaata gactataatc tatatcagaa gtcacttcta cttctgcttt tcttaaaact
    29161 gcacagcatc tagcatgtgt atactgaaca tatggtccag tctcaccatc aaaactaagc
    29221 gttctttccc aagagaaggt atagtcttta attctactgt tagataactc ttggaatact
    29281 actgccccaa cacctacttg cttagatatt tcatccacat ttttagcatt tggattttta
    29341 gctagtataa tttccttagt tttatctata gcttgattta aagcatcttc taaaaatact
    29401 actcttcctt ttctagtcga catagtacct tcttcaagtg caaccatacc aaatccaaca
    29461 tgaattaaat cttttgccca atcatatccc ataagctcaa ttaccttaaa ccattgttgg
    29521 aaatgtaagt tttgttgaga accaactaca tatatacact tgtcaaaatc ataagtctct
    29581 tttctatata ttgcagcagc taaatctctt gtcatatata acgttgaacc atcattctta
    29641 gtaataagtg ctggtggcat tttatactct tctaaatcaa caattcttgc tcctttagac
    29701 tcttttaaaa gatttttctc ttctaatagt tcgataactc tgttcatttt atcagaataa
    29761 aaactctctc ctgctaaaga atcaaattct atatctaaaa gtttatacac tctgttaaac
    29821 tcttttaaac tctcatttct aaaccattgc cataattctt gtgcttcttc gtcaccattt
    29881 tctaatttat taaaccatgc tcttgcttca tcttccattt ctgggtgttg ttcagcttca
    29941 tcatggaatc ttacgtataa ttttagtaat tctggaattg gattagactc tactacttct
    30001 ttttctcccc actttttaaa ggccactatt aacttaccaa actgagttcc atagtcacct
    30061 aaatgattta ttcttatagt cttgtatcct tgcgaatcat atatcttata taaagcatta
    30121 cctataactg tagttcttat gtgccctata tggaaaggtt ttgctatatt tggagaagaa
    30181 tattcaacta ttacagtttt atcttttcca aaatcactat gtccgtaatt ttctttttca
    30241 tctaaaactt ttttaattac agtttctgct aattgagatt tatttacaaa gaaatttaca
    30301 tatccgccta attgtattac ttttgttatc tctccacttg gttctatact ttccgccaat
    30361 tcacttgcta tcatgtttgg agcttttcta aacactttgg ctaatttaaa gcatggaaaa
    30421 gcatagtccc ccatatcctt atttggtgga acttctataa gtgcctctat ctcttctttc
    30481 gataaatctt ctattttttc tttaaggcaa tttgatattg ccaccttaaa atcttgcatt
    30541 ctgttgctcc tctctacatt gttcctggtt tatattgtgc ttatctcttt atgttaaatc
    30601 aaatttcgtg ttttttcaag tatttcatta cagtgtaaaa cactttaata attatgtttt
    30661 ctgtatatat taaaaatact ataattgcag tttttaatca cactattatt gattttttat
    30721 aagacttttt tctattttat tgtttcttgt actatccagt tgactttata tacttttttt
    30781 aatcttttat caattttcat acatcaatat taattttata tacatttatt ttgctctttt
    30841 atctattttt atacatcaat attagcttta tatacttttt ttactctttt atctattttt
    30901 atacatcaat attagcttta tatacttttt ttactctttt atctattttc tataaatctt
    30961 tttcactctt aatttttatt ccttcttctt ctaacagcct tgctgttata cctttccctg
    31021 gtatcttttt acctgaaaaa ctaccatcat atattagatt acatccacaa gaggggctac
    31081 cctcttttag tattgcttcg ctacatacat ataaatttgc tatttttaga gtttcattag
    31141 cccctcttac aaagttttct gttacatcct tattatcttt agtaattact tttccatcaa
    31201 ctatctcagc aggtggtctt ggtgtagata gaccacctaa ctgctctggg catacaagtg
    31261 taaattcttt gccttttaaa tattccttga ctttttcatt ttcattatta tcgccactat
    31321 atttacaatt tattcctatt aaacatgcag ataccaatat cataaaaatg tgctcctata
    31381 caaattctta atatattctt atacaaatta ctaatgtatt cttatacaaa ttattttaat
    31441 ttaacaattg ttactcctgc tccaccttct ccatattgac cacctcgttg tgattttaca
    31501 tgtctatttc ttcttaaaat gtcttgtagc cctgctttta atactcctgt gcctattcca
    31561 tgtattactg ttacagactc taaacctgcc acatatgcat catcaagata cttttctact
    31621 tccattatag cttcttctag gtttaaccct ctaaggtcaa cttcattttt tacactacca
    31681 gattttgacc ttataatgtt tctcgttgat ttagttacat ttgttgaaac atctttgcgt
    31741 gttttttgta aagatttaaa aggtagagtc atcttcatta taccaatttg cactactgct
    31801 tcttttctct ttttatctac tgaaactaca ctaccatttt gatttaaagt tattacctta
    31861 acttcttcac ctggctttaa gtcttttatc tccttatttg atacctttgg aactatcata
    31921 ctttttactg ttggctgaag acttcccata gagctggtca attcttttct aagttcttct
    31981 atcttacgat tcttttcttt tgaagctctt tcttgttcaa gatttctaag ttcttttatt
    32041 ataatatcta cttcttcttt agcttgtctt ataattgaaa atgcttctga tttagctttt
    32101 tcaatcatct tatctctttg actgaccaat ttttcaagtt tttcgtcata ctctactttc
    32161 agtttttcaa tctcttcttt taatctttca gcttcttctc tatcttctac tgctttgatt
    32221 ctattctttt caacattctg taacacatct tctaaagcta tgttttctgt atttatatat
    32281 tctttagcac gtgaaatcac ataatcactt agcccaagtt ttctagaaat ctcaaacgca
    32341 tttgattttc cgggaacacc tataagtaac ttatatgttg ggcttaaagt ttctatatca
    32401 aattcaactg ctgcattttc tacacctggt ttagtaagag cataattttt aagctcacta
    32461 tagtgagtgg tagctataca tttagctcct acagaattta tatcttctaa tactgctatt
    32521 gcaagggcag ctccttcaac agggtctgtt cctgctccta actcgtcaaa tataactaaa
    32581 gagtctgctg ttacgttttg taatatagat actatattcg tcatatgaga tgaaaatgtt
    32641 gatagactct gttcaatact ctgttcatct cctatatctg caaatacatt atcatataca
    32701 cacatactac ttccataatc agcaggtata tgaagtccac tttgtgtcat aagtgcaaat
    32761 agacctacag tttttattgt aacagtctta ccacctgtgt ttggacctgt tatcaccaat
    32821 gtatggaagt ctcttcctaa ataaattgta tttgctacta ccttcttttt atccagcagt
    32881 ggatgtcttc cattttttat gtttaaatat ttatcctcat ttaaagttgg ttctattcct
    32941 ctcatctgta tagaaagttt tccctttgaa aatgcaaagt ccaatcttcc aagtatctct
    33001 tgattagaga ttaaatcttc gctcacttct ccaaccatag ctgaaatctc tgataaaatt
    33061 ctttctattt cctcttgctc tcctagtttc aactgcctca attcattgtt catctctact
    33121 atagtcattg gctctataaa aagtgtcgca ccagaagatg actggtcatg cactatacca
    33181 gctacttgcg acctatattc agattttaca ggtactacaa atctatcacc tcttagagaa
    33241 attattgcat cttgtaagta cttttgataa gtagttgaac ttattattga atttaatttt
    33301 gacctaatgg attgattttt ttgggctatt cttcttctga tatctctaag tgtacttgaa
    33361 gcattgtcag atatttctac ttcactaatt atagcattgt atatctggtc ttctatgtcc
    33421 ttgtacacat atagagaatt tgatagagat tgtattattg gataattaaa atcttcttca
    33481 tcactactag ataagctatt tcttaatctt ctagccactc ttaaggtatc tgctatcata
    33541 atcagacttc ctgggtcaag agaagcacct atataagctc tctttacctt atcttctata
    33601 tcatgaatac cttccaaccc tactgaacct ctctttataa taattgattg agcttcacta
    33661 gtttcttcta gcatagattt cacttctaca aaatcagtat ttggtactaa attttctata
    33721 tattttagac ctaaagaaga tgaggctttc ttctttaaca aatctattat tttattgtat
    33781 tctagaactc ttaacgattt ctcattcatt ttctatattc accatcctcg atattttaac
    33841 ttttttattt aattttaaaa atgtatgttt tcaagcattt ccaataaaaa atgcaagttt
    33901 tatttctttt tcatttctaa aattattata ttttttctct tttagatata ttttacacat
    33961 attttgtatt atactatgta tgtagctatc tatatattat acataaacat gctttatttg
    34021 taatataatc tgttaaaaaa tcttctttaa tatttttaaa ggagtttatt ttagcttata
    34081 aaaaaatagc tacttatata gataaattaa ggaggaacat gagtcatggg aaacgtatta
    34141 gaaggattaa aaccagaaag tgtttttaaa aactttgaga aaatatctca aattcctaga
    34201 ggttctggaa atgaaaaagg cataagtgat tttttactta gctttggtaa aaatttaggt
    34261 cttgaaacta ttcaggatga aagcttaaac ataataataa gaaagcctgc tacaaaagga
    34321 tatgaaaatt gtccaggtgt agttcttcaa ggacatatgg atatggtttg cgaaaaagaa
    34381 aagaatgttg aacatgattt tcttaaagac cctataaaat taagaattga tggtgacatg
    34441 atatatgcta ctggaactac tctaggagct gacaatggta tagctgttgc tatgggtatg
    34501 gctatattag aagataatac tttagagcat cctgcactag aagttttagt tactgttaat
    34561 gaagaagatg gtatgaatgg tgcagatgca ctagacccta gcttaattaa aggtcaatat
    34621 atcctaaata tggactccga ggaggaagga tacttattag ttagctgtgc tggtggtaaa
    34681 acttgtgttg ttagtttacc tgttgaatac aaggaagtta aaggtgacaa acaaggatta
    34741 ttagttgagg ttactggact attaggtgga cactctggta tggaaattgt tttacaaaga
    34801 gcaaatgcta acaaagcaat agctagagta ttaagtgtat tgaatgttga ttatgaatta
    34861 gcttctgttg atggtggtac taagcacaat gctatacctc gtgaagctaa atgtgtaata
    34921 gctgttaata aagctgatgt tgaatctgct aaaaaacaaa taaatgacat tttaactgca
    34981 tttaaacatg agtttactac ttctgaccct ggcatgactt actcagtagc tgaaacttct
    35041 gtagacaaag ttcttactaa agattgtaaa gaaaaagttg ttcaaatgtc ttgcttaact
    35101 cctcatggag ttcaatcagt aagtcttgat atagaaggat tagttgaaag ttctactaac
    35161 ttcgctataa ttgaaactaa agaatctact attgaatttt taacttctgt tagaagttct
    35221 gttatgtcta ttagagatga aatcgctgat agaataagac ttttggctca agctttaggt
    35281 gctaattacg accttatagc tcaataccca gcttgggaat ttaaaaaagg ctcaaaatta
    35341 gaaaaaatat gcagtgaaac ttatgagaaa ttaactggta aagttcctac tgttatggct
    35401 ctacatgctg gtcttgagtg tggattatta cttgataaac ttcctcatgc tgaagctatc
    35461 tctataggac cagatatgtt tgatgttcat actccaaatg aacatgttag tatcccttct
    35521 gtagctaatg tttgggatta tgttattgaa atattaaaat ctatgaacca atattaaatt
    35581 taagttttga ttctaaatag attttaattt taagtaatct atataaaatt aactctgtaa
    35641 attaatacta ctgttagtat aatatttact ttcaatatta acttatagaa ataaagccta
    35701 atgatagata ataacatgta tcattaggct ttattgtaat ttaaatatta ttaaacttta
    35761 ttctgtttta aaattaattc ttatactatt ttaaatctat ttcttccttg tctcttagaa
    35821 tcataaagag cattatctgc tatttgatac agttcatcat actcttgacc acgctttgtt
    35881 ataactaccc ccatactaat agataaactt tctccatttg gcaacttaat gagatgaata
    35941 tgattgtata catcattaca tttattgata agttcttcct tatttattat atcatttata
    36001 taaacactaa attcatctcc acctaaacga cctactatat catcttttgc aaaactttca
    36061 tcaagtacct ttgcaacatc aatcagtact ttatctccta ccaggtgacc ataagtatca
    36121 ttaattgact taaaataatc aatatctata atcaataaag ctccattttt ttcagttgat
    36181 aaatttaata aattttgtgt agttaatttt ctaatatatg ctgcattata aatatttgtt
    36241 aaactatccc tttgaacttt atccaacaat ctattctttt cctctttttc agaatcaata
    36301 ttagctattt taccaattac atacattggt atttttctat cattataaat tatttttgaa
    36361 gtaattctaa tccaatgaaa agtatcatca agcatgatag aatacatttc agtagtccca
    36421 ttttcttgtg attttattac atcaaaaaac tttttattat attctcttct tattttatct
    36481 ctttcatttt ctatattgcc attttcactt tccttatcac ttttatccaa accataataa
    36541 gtctcaatac ccttttctgg catagaaatt atcaatttat cttgcttata atcatactca
    36601 aaaaagtatt cacttgtcat ttcatacaac tgagtatgtt ttttgagttc caaagatatc
    36661 ttttcgttca tacttgtacg agtatgtaaa ccaaatgcca taaatccaat aatacaaatt
    36721 aataaacttg ctacaataaa aatagtctca attggattct gttgcataat ataaagcaac
    36781 gagaaatctt gtctatatgt agtattttgg tagacaattg attgcatttt ttctgatgaa
    36841 atacttataa tagctttatt aaatatattc aataattctt gtttatcttt tttaattatg
    36901 ccaaaacaaa tactctttgc ttcataagtt tgaggaacta ggcgcacatt tttatactta
    36961 ggttgattca tatagtactg catagaataa ccttctccat aagtgtaatc tgcatagcct
    37021 ttattaacag cttctataca ctcttctaaa ttatcatacc aaactatatt ttcttcaaac
    37081 tgaccataat aatcaatacc tttggctaga gctatacgct ttccatttag actattctca
    37141 tttaatttat tattaatcat catagtatat tgagtagaaa gaaaaggccg agacatagct
    37201 atattatgtt cacgagagta ctcataatca taaatcattc ctgctacaat atcaatttca
    37261 tgttttttag ccatatcttc caaatcttct tgagatttta caggcacaag ttcaaattcc
    37321 aaacctgttt tatcacatat atgatttaac aaatcaattg atatacctct taattctcct
    37381 gtatctttat ctgcattttg aaacggtggt ttgtttaaaa gtactcctac ttttaatttt
    37441 tctgtatctt taatgtaatt agtttcttca tcagataaaa acatttgatt attctctggc
    37501 gtaaagtatt tctcatataa agtagttgaa aaatatgggt ccttctgttc aatattatta
    37561 attgctgaat taagcttttg agttaagtct gtactgcctt ttggagtaat gaaataaaat
    37621 ggctttgatg agaattttgc tacagtacgc acaccttcta ttgggttcat actaacatta
    37681 agaagtacat ctgctttacc actctttagt acttctaatt gctcgtcact tgttttacaa
    37741 tataccaatt caggtgacat aagattcatt ttacaatact catctaattc tttaagccta
    37801 gtatcagaat tttctattac agcaatacga atttttttca catcttgcga actcattatg
    37861 ttatcaatat ctgtattttc atataatgct tgtagtactg tatttgctac tccataactg
    37921 tttcccgcat aattatatat tttttgcatt ggctcattgt ataacatacc tcccattata
    37981 tcaatttcac cattttctaa catcttcatt aattttgtaa tagattcatt tatctctcca
    38041 ggaacttgaa caaattcata attccatcca gtatactgtg caatttctag caaatattca
    38101 tatgtatacc caaaatagtt accttgctca tctatttcag ttaaaccttt ttgaatagga
    38161 taagcaacac gaatggtacg attatcatta cctatagcat tgataccaaa agaaaatatg
    38221 caagaagtga atatagatgt aattacaata attaataaaa gtaatttttt taaattcact
    38281 acacaatcct ccatatagtt aagatttaca gtataaatta ctgcataaac ttgtagacat
    38341 gaaaaaatag attagtatat aaaaggatat ataaatctac ttagaaattt aaatatcctt
    38401 aatatataca atatatttaa attataactc atttatatat ctattacaac aaatttaccc
    38461 tgctaaatac ctactctata tcccataatt ttacaagttg taatgaagtt taagttctat
    38521 ctcatttcaa atttagcagt gtttctattt acagttcttt tatatttcac atctggataa
    38581 cccaatataa atgaagtgat aactgcttcg ttctcgctca ttcctaacag ttcttttatc
    38641 tttggctcta catttgatgc cataacaaaa aatccattat aacaaattcc taatccttga
    38701 gaataggcca taagttccat attagatgca gccaatcctc catctacata agcacttcca
    38761 cctagagaca tatcacctac aactatcata agtagtgggg catcataaaa taaagaatca
    38821 tttccgttct ctttatatct tttgtacatt ttcttaaatg tatttttata gatagaacgt
    38881 acagggtcat tgtcatcagt atctaatgcc aaattataaa gaccttgtat tgccagttct
    38941 ttcacttctt ttagcttttc ttttactaat atgtatctta taggctgtct attaccacct
    39001 gttggtgtgt atctaccagc ttccactatg ttttttattt tttcttcttc tacctctata
    39061 tctttatatt gtcttatact tcttctaaat ttaataaaat tcaataactt ttcgggttct
    39121 aattcaaaac tttcacgatt gtattctatt acttcatttt tatcaaatcc ttgcatttct
    39181 attgcatctg ttgggcaaat tgccatacag tgaccacagt taatacagag aatattttta
    39241 acctttgctt tatcatcctt aacttttatt gcatttacaa gacagtctga ttcacacatc
    39301 ccacagccta cacatttttc taaatttatg tctatcattc caaattattc ctccttttaa
    39361 aaaatttact ttagtataaa acaaatagtt gtctaagtca atttttattt ttttaacaat
    39421 atactagcct taactacact ttaatggaga aaaatataca taaaattaga acttaaatta
    39481 gaatttattc aaaataaaag ataaaagaag taaatgtcta aaagtgaact ttttagttaa
    39541 tgagacattc tttattgtat gaaatcaagt ggatatttat catttcaaca ataaaaaagg
    39601 cttacctcta ttttggatat gtccttgtta agtagacaga ctaaaaaagg ctatctttat
    39661 gcactgagat agcccctttt cccaactact attgtttatt ttaatattat atcaataaat
    39721 ttaatttgct ataacctgta agttacattt taatttgatg taactttaaa ttacattttt
    39781 aagtaatatc tttaaattat attcttaaaa tatatcatta aactacattt taattaatct
    39841 aaaaactata tcttaattaa tctaagaaac ttacattata atcttttagt ttcccattat
    39901 catatacaat ctttgcacta aagatatttc cagtcttagc ccaatttaag aacaacctgt
    39961 caccttccca catatttaaa tcgaatattt tatcattacc aacccaaatt aaatttcctt
    40021 ctttacaatc atttaaatct ttttcagaca attcaccttc aaactcatct gagaaataca
    40081 aaaacatata ccaatcttct tcatctttaa attttggaaa agttatcaac cctttatatg
    40141 acaatgattt aagagtaaga ccagtctctt cctttacttc cctaattaca cattcttctg
    40201 gagtctctcc ttgctcaaac tttcctccaa ctcctacata cttaccttca tgtatatcat
    40261 ctttcttttt atttctgtac aacatcaaag tcttgtcatc tttttctata taacaaatag
    40321 ttgtaagttt catgtatctc gccttacttt ctctttatat tctcaattaa ttattttcag
    40381 cactttcaag cacatcactt atattcttca ttgcattaag agaaagttct gcaaattcat
    40441 caaatggaat ccctatatct tctatacttc tcattgcatc tctatttaca tttgaagcaa
    40501 atctcgtttc tttcattctt ttaataacag acttagtctt tacactagta agttttttat
    40561 ctggataaac taaagctata gcagttataa acccagttat agggtctgct gcatatattg
    40621 ctttttccat taagctagtt cttgtaactc ccattccatc atgatgacca agtattgctc
    40681 tatacatttc ttcgtcaccg aagttttctt ttttaagcag ttctactgtt gttgtagcat
    40741 gtccttcaca tatttctcct ggctttcttc caactatatc tgaatccaaa tcatgtaaaa
    40801 gacctgccac tgcccactct tcttcttttt ctggctctag tcttttggct aattctctca
    40861 taacagcttc aacagcataa caatgttttc tcattctatc agtttctaaa tacttaaata
    40921 aaatttctaa agcttcttct ctcattttca ccaatccccc tgttttttca catatatgat
    40981 atttttatat attaatacac ataaattcaa gtctttatat atattcatgt atatttttca
    41041 tgttatatct attctacacc tttatagtag tgtcctgcaa ctgttccaat ttcatctaat
    41101 ttcttataca aatctgtagc cttttttcca agtttataat tattactcaa tacctcaatg
    41161 tgagcttcaa taaccttttt aaccatctca ctatcttcaa ctgtaaaatc tatcctaaat
    41221 acatctattc caatttcctc taactcatat agattgtcca acatacaagt aaccttcgaa
    41281 ttgtaaattg tacttctaca gaatatatct tgtgttatcc tataacttgc atccgtaaag
    41341 tctcttaaag cataactact ttccttacat ttagcaactc tcttgtcctt tttacaatct
    41401 ctaacaacta ctcccatagg acagtattct gtaaccatca taggagcata tccatatatt
    41461 actgcttcaa tttctacatc tgtatatttt aaagtttgtt taatttcatt taagtttagt
    41521 tcttgtgaca aacaaactgt acttgctccc tcttctttga agtggtttat actttcacta
    41581 ttgaatgcat ttaagtagca atctatattt aagtcttttt tcttaaagta ttccatagag
    41641 ccccagttac caatttgaac cttattaatt ccttttatat tatccaatat ctcaaattgc
    41701 ttgtactctt tatttctaac aatccttggt gctgaataga ttaatttttt gtcagtgact
    41761 gctgtattat agtggttctt attcaaagta tctattttac ttgtatttga caaagtatct
    41821 atgttatttg tatttgaacc cattagttct ttaggaatac ctaaagacga agaagtatct
    41881 aaatccatag aaatacttga atctatagaa atacttgaat ctatagaaat acttgaatct
    41941 atagaaatac ttgaggccat ttgtaaagct tttgataatg tatttatatc ttcataatag
    42001 attaaatcta cattataatt taatactgct tctaattgct ccaagttctt aactttaact
    42061 ctaatcttag gaactgtatc aagttttttc tgtttattta ctctatttgg ttggtatttt
    42121 aatctattct tcttaaattt tctatctttt atctttattc tttcttcatc taaaagctca
    42181 atacaatccc ttctcatttg atttaacaca cttataggca tacttacacc ttcatccagg
    42241 tcaatttcta tctcttttaa cttatatggt gtatttccaa gcttttgaat ttgtgtttct
    42301 attttttctt tacttaaagc cacctttata gctttttcaa ctattttgtc tccttctacc
    42361 accgatttat ttccacaaaa atcacttata aaaagctctg gctttttacc tagtttaata
    42421 ctcacttttg cacgtatatt agtttttatg aactctttat cttgctgaaa tgtagcttgt
    42481 actctattca ttaattcgct gtcagaagtc ttatatataa gctgatttct ctttgcttct
    42541 ccaacaaaat ctaattctat caaatcccct ttatgtgcta ttgtagttat ctctccatcc
    42601 tttattattc ttccaatagt acctccacct aagttaattc catcaccttt cttaagcgta
    42661 ttttctagca ttactctaag tcttttgttc tttctattat aatctaaaac ttttccaata
    42721 tatagccctt gattattagg tctattagag ttcattatat ccttaccaac ttcacctaat
    42781 atatatcctt ttgtaaactt tctattaaat atggtataca aattatttat tatttcctca
    42841 gaaatactta tttttttctt atctacaaat tcattaatcg cttctctata tcctgaaacc
    42901 acagtagcta catactctgg ttttttcatt ctcccttcaa tttttaaaga taatacacct
    42961 gtttcaatta ttttatccaa atcttctata gtacttaaat ctctaggact caacaaataa
    43021 tctccattac tttttactac ttctcctgta tttatatcta tcaactcata cttttgtcta
    43081 catggctgtg cacacctacc tctatttcca gacctattgc ctatcatact actcataagg
    43141 cattgacccg agtaacatac acataaagct ccatgtacaa aaacttctat atctacagtt
    43201 gtattttcac aaatccattt tatctcatca acatttaatt ctcttgctaa aacaactcta
    43261 tcaaacccta cactctgaag gtattttaca tcttctaagg aatgtgctac catttgagtg
    43321 cttgcatgta gttcaaagtc aggtaatagt ttttttataa gtcttgccat tcctatatcc
    43381 tgtactatta aagcatcaat atctatatcg tataaaaatt ttacatactc tacaaaatct
    43441 tctatttcat actgttttat caatgtattt acagtaacaa agacttgcac tccccttata
    43501 tgagagtatt taacagcctc aatcagctca tttctatcaa agttatttgc agatgctcta
    43561 gcactaaagt ctttaccacc taaatacaca gcatttgctc cgttttgaac tgctgctttt
    43621 aatgaatcaa aagagcctac tggtgcaagt aattctattt ttttcatatt tttctcctct
    43681 atttatactt gttaggttaa tataattata tctataactt aaatatttgt attaaattta
    43741 tatatttctt actatatttt aaattcttca attaatttat atattagtta tttatatttg
    43801 gtccatttga gttaacatat acattattta ctatatttag tatatttcta ttaacttata
    43861 tattgattac tgtattttaa attcttcaat taatttatat actgcttact aaatttaaaa
    43921 cattttaatt aatttgtaca ctgtttacta tacttgaaat acttctatta atctatatat
    43981 tgcctactat acttaagaca ttttaattaa tttatacact gcttactata cttaaaatac
    44041 ttctattaac ttatatattg cttactatat tttaaattct ttaattaatc tatacaatat
    44101 ttgctatata taaagtatat caaataacag cttcattttt ttgtaaattt acagaaagtt
    44161 aattttcatt aaatgaatca tctttattga taaacataat tccaaagacc actgccaaaa
    44221 taaaaaaaca tattagtatt gatttttcca cgaaaatttt tgtaaaaaaa gttcctaaaa
    44281 tagctccaga tataaagaat ataataatag cataatactg taaactcttt tttatcataa
    44341 aaatattctt agtatgtata taatgaaata aagtttctgt agcacttctt aaatttccag
    44401 tacacattgt cgttgcaaaa ctattcccat ttacttttct aaaactctct acttgcattg
    44461 aacaaataaa agaaatagca acatttacaa acatattaaa tcttcctcta ggaataaatg
    44521 aaacaataag tattgtaatc atttcaatta taatgataac ctgacgccaa tgaatctttg
    44581 tcttattatt tgtaaaatac tttcttatct tctcggctat aaatacacct aacataaatg
    44641 ccaaaatagg aatcaaataa taaaatgatt cctttacatt accttctgct atattaagcc
    44701 caaataaaac tatatttcca gtttgtgcat tagcaaagac tttacctctt accacataag
    44761 tataaatatc caaaaatcct cctgttattg ccaacaaaga acataaaaag aatgattcag
    44821 acatctgact cttgcttgtc atatataaac ctctcgcttt ctaatgatag atacaaaatc
    44881 aaaatcattt tagattctat ttttcattga tataaaaaaa ttatttatat ttaaatatta
    44941 tctatattta tacatcattt atatttatac attatttgta ttt
//
LOCUS       DAEPOZ010000006        44376 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.006, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000006 DAEPOZ010000000
VERSION     DAEPOZ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44376
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.006"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..442
                     /locus_tag="KR440_000276"
     CDS             <1..442
                     /locus_tag="KR440_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBH0697345.1"
                     /translation="VVSSLLISEMAAFYYSKGISLYDGLIELYKKYGFFKEQTISLTL
                     KGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKSNVLKYFLEDESWV
                     AIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            656..1495
                     /locus_tag="KR440_000277"
     CDS             656..1495
                     /locus_tag="KR440_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBH0697346.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
     gene            2012..3868
                     /locus_tag="KR440_000278"
     CDS             2012..3868
                     /locus_tag="KR440_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBH0697347.1"
                     /translation="MDIHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYRGGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIIDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALINKIFESDKGVVENKKVVTFELNVSINDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSENNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    3951..4191
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    4257..4492
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4577..5794
                     /locus_tag="KR440_000279"
     CDS             4577..5794
                     /locus_tag="KR440_000279"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBH0697348.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRSIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            5908..6402
                     /gene="cdeM"
                     /locus_tag="KR440_000280"
     CDS             5908..6402
                     /gene="cdeM"
                     /locus_tag="KR440_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HBH0697349.1"
                     /translation="MENKKYANGGYSEDWYERGESTAKWFQNDREEYERAAYDEDRER
                     RGSNCGCSDSGGNRPRNCERFRREAEIREREAREAFCESSERKKEALAYECEARKLWE
                     EAEKYWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCN
                     CCRK"
     gene            6667..7932
                     /gene="hisD"
                     /locus_tag="KR440_000281"
     CDS             6667..7932
                     /gene="hisD"
                     /locus_tag="KR440_000281"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBH0697350.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(8135..8887)
                     /locus_tag="KR440_000282"
     CDS             complement(8135..8887)
                     /locus_tag="KR440_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0697351.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYVE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANKKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIHIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(9005..9274)
                     /locus_tag="KR440_000283"
     CDS             complement(9005..9274)
                     /locus_tag="KR440_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697352.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            9749..10843
                     /locus_tag="KR440_000284"
     CDS             9749..10843
                     /locus_tag="KR440_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBH0697353.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVES
                     LRERSINGVISMCLKETDYQYSLSNKKGGY"
     gene            10845..11795
                     /locus_tag="KR440_000285"
     CDS             10845..11795
                     /locus_tag="KR440_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBH0697354.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGLKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINNNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKIDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            11788..12972
                     /locus_tag="KR440_000286"
     CDS             11788..12972
                     /locus_tag="KR440_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697355.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYEKNNIDKKSLMREMNNVSKGVIDNEDTPSGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKSLLEISSYFKSKGLKIGIDTFSPFISYFTGQDLKKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIIAPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            12985..14481
                     /locus_tag="KR440_000287"
     CDS             12985..14481
                     /locus_tag="KR440_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBH0697356.1"
                     /translation="MSKILEMQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNSHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEEYMKICEDFDFNIDPHTVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSEGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            14504..15454
                     /locus_tag="KR440_000288"
     CDS             14504..15454
                     /locus_tag="KR440_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0697357.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMLLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLVSFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            15475..16437
                     /locus_tag="KR440_000289"
     CDS             15475..16437
                     /locus_tag="KR440_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBH0697358.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            16809..17441
                     /locus_tag="KR440_000290"
     CDS             16809..17441
                     /locus_tag="KR440_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBH0697359.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            17852..17944
                     /gene="kdpF"
                     /locus_tag="KR440_000291"
     CDS             17852..17944
                     /gene="kdpF"
                     /locus_tag="KR440_000291"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBH0697360.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            17985..19667
                     /gene="kdpA"
                     /locus_tag="KR440_000292"
     CDS             17985..19667
                     /gene="kdpA"
                     /locus_tag="KR440_000292"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBH0697361.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGIVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            19692..21749
                     /gene="kdpB"
                     /locus_tag="KR440_000293"
     CDS             19692..21749
                     /gene="kdpB"
                     /locus_tag="KR440_000293"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HBH0697362.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNVGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMIL
                     NLG"
     gene            21768..22394
                     /locus_tag="KR440_000294"
     CDS             21768..22394
                     /locus_tag="KR440_000294"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBH0697363.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNASYGGVSSGSFNY
                     GATNPELHDRVQKDIEKFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNIEVAKILGLI"
     gene            22631..23539
                     /gene="cysK"
                     /locus_tag="KR440_000295"
     CDS             22631..23539
                     /gene="cysK"
                     /locus_tag="KR440_000295"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBH0697364.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            23566..24156
                     /gene="cysE"
                     /locus_tag="KR440_000296"
     CDS             23566..24156
                     /gene="cysE"
                     /locus_tag="KR440_000296"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBH0697365.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            24316..24504
                     /locus_tag="KR440_000297"
     CDS             24316..24504
                     /locus_tag="KR440_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBH0697366.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(24558..25427)
                     /locus_tag="KR440_000298"
     CDS             complement(24558..25427)
                     /locus_tag="KR440_000298"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HBH0697367.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDMNKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(25624..26868)
                     /locus_tag="KR440_000299"
     CDS             complement(25624..26868)
                     /locus_tag="KR440_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697368.1"
                     /translation="MENNLNIAKKNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDSLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYYMENNTNITIYENNTLQYKFDEESEDIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKSRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
     gene            27159..27470
                     /locus_tag="KR440_000300"
     CDS             27159..27470
                     /locus_tag="KR440_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBH0697369.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQL
                     T"
     regulatory      27563..27655
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            27755..28549
                     /gene="thiD"
                     /locus_tag="KR440_000301"
     CDS             27755..28549
                     /gene="thiD"
                     /locus_tag="KR440_000301"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBH0697370.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            28573..29361
                     /gene="thiM"
                     /locus_tag="KR440_000302"
     CDS             28573..29361
                     /gene="thiM"
                     /locus_tag="KR440_000302"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBH0697371.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGIYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            29384..30016
                     /gene="thiE"
                     /locus_tag="KR440_000303"
     CDS             29384..30016
                     /gene="thiE"
                     /locus_tag="KR440_000303"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBH0697372.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITERYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEATCAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            30155..30457
                     /locus_tag="KR440_000304"
     CDS             30155..30457
                     /locus_tag="KR440_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0697373.1"
                     /translation="MIKILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRVPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(30523..31272)
                     /locus_tag="KR440_000305"
     CDS             complement(30523..31272)
                     /locus_tag="KR440_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0697374.1"
                     /translation="MLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMNNI
                     NINNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQLLE
                     AILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLPNE
                     IVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETILI
                     GDILPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(31266..32228)
                     /locus_tag="KR440_000306"
     CDS             complement(31266..32228)
                     /locus_tag="KR440_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0697375.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVKMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(32232..32690)
                     /locus_tag="KR440_000307"
     CDS             complement(32232..32690)
                     /locus_tag="KR440_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBH0697376.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            33046..33402
                     /locus_tag="KR440_000308"
     CDS             33046..33402
                     /locus_tag="KR440_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0697377.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            33474..34190
                     /locus_tag="KR440_000309"
     CDS             33474..34190
                     /locus_tag="KR440_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0697378.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            34183..35007
                     /locus_tag="KR440_000310"
     CDS             34183..35007
                     /locus_tag="KR440_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0697379.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQNILFFGMLGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIVLITMIYGFLISQTATLFICKYIFNFIFRNIPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKFYVILITLLIIAG
                     LYFSTIYINIINLFRKYYNLIGFQFNNLVAGLLSFLIIMFILNGISYLLIKKKVSL"
     gene            35019..36218
                     /locus_tag="KR440_000311"
     CDS             35019..36218
                     /locus_tag="KR440_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697380.1"
                     /translation="MKLKSKISILIIFLTLAIYFSIGLLNNEDNLKIKTIKGDSNELD
                     HININFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNEESKDINFVIDEKKEDLEKGVFPSEGSDKEIEYEKLDMNNFTRTRYKVDKSTDD
                     YYANILASKRYKKYLYVSVLYATSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLS
                     NYFSEKYQDIENIGVSYASKYEDKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDN
                     REKRGIRSIEIDSNKIVAYTSRLNSILSWDNKFMLYIINKNSKEIVYEGELQFNSSNS
                     MLEFEIK"
     gene            36227..37039
                     /locus_tag="KR440_000312"
     CDS             36227..37039
                     /locus_tag="KR440_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697381.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVNVTGFSSFRNIMGSTESWFIFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     LFLVLEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            37050..38318
                     /locus_tag="KR440_000313"
     CDS             37050..38318
                     /locus_tag="KR440_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697382.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNKNPKSRIRNDYLNIDKKYSDFVNDKG
                     KELIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISGSKVNNICILKIDIKNKSYKLLNRFNLGLQNG
                     YQQNINEIKFSIGSKLYLEIVNNKSKNGDIEGHDSYSNLIIYDIEDNTFNISNKFMDN
                     LNKEKNANPKEIGLLSKTKLDYMIKNNKLNLIVTDMSNSIINLVYDINSNEIKLEDYE
                     ILDLKVDYNEASSGVRKVKLLDDKIYSISGMKNSISGELKGFKGIVDVVGTKPVKFQV
                     FDMKLKKNIYEAELINGDISLQEKLFFVAN"
     gene            38684..39865
                     /locus_tag="KR440_000314"
     CDS             38684..39865
                     /locus_tag="KR440_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBH0697383.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQVDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(40007..40924)
                     /locus_tag="KR440_000315"
     CDS             complement(40007..40924)
                     /locus_tag="KR440_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697384.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
     gene            41177..41971
                     /locus_tag="KR440_000316"
     CDS             41177..41971
                     /locus_tag="KR440_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0697385.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            41972..44032
                     /locus_tag="KR440_000317"
     CDS             41972..44032
                     /locus_tag="KR440_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBH0697386.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFACYIKADFKEVFSLTVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPAICEELFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT        17241 a         4676 c         7562 g        14897 t
ORIGIN      
        1 tgtagtttct tcattgttaa tatctgaaat ggcagcattt tactattcaa aaggtataag
       61 tttatatgat ggtttaatag aactttataa aaagtatgga tttttcaaag aacagactat
      121 atctttgact ttaaaaggta tagaaggtgt agaaaagata aaagaaataa tatcttattt
      181 tagagaaaat caaattgatt atataaatag tattaaagta gttgataaaa aagattataa
      241 aaatggtata gataatcttc ctaaatctaa tgttttaaaa tactttttgg aagatgaatc
      301 ttgggtagct ataagaccat ctggaacaga accaaaactt aaattttaca tagctgtaaa
      361 aggagctagt gatattgaag cagataaaaa gattcaaggt ttgaaaaagt atatagatga
      421 catggtcgaa aaattaaaat aaaatattat tattttataa gataattttg taataatatt
      481 tatttagctg aaaattataa tttactttca ttgagtagaa tatggaaatg ttagtagaat
      541 tgttattttc agcttttatt tgtgttattt ttacttaaat aattatatta tagaatgaat
      601 ctgagtataa ttaatattgt ataatgaaat taatttgata gaaaagggga tatttatgtt
      661 tggtatagat aagaaattga ttgaatatat aattgcaaaa tccaattaca gtgaattaag
      721 ttcaaagtca gcagaagtat ctttcttctt aatgttgtcc atatttcctt ttttaatatt
      781 tacaataagt tctatagctt atataccgat tcttcattta aataagtata tagctttgtt
      841 tagaaatatg atgccagaag gtgcttttgc tgtgttatct tcaatcatag tttctgcaat
      901 agacaataga aacctgaaat ttttagcagt cagttttgta cttactatgt ggacattttc
      961 aagagcagta aaagctctta ttaaaggaat gaatagagcc tataaagtta aagaaactag
     1021 gtcttttttt aaaattttat ctatatcatt tttatttaca ataatgcttt tggtccttat
     1081 ttttttatct atgatatttt tagtttacgg ggaaaaaata ggttatttta tttttaatct
     1141 tgtaggattg gatgagatat ttataaaaat atgggatatt ctaagataca ctgtaggtat
     1201 aataacaata atagtgatat ttacactttt atataaatac actccaaata aaaagttaac
     1261 tattaaagaa tctgcgccag gtgctatttt tgctactttt gcttggtttt tagtatcatt
     1321 tttatattct tattatacaa attactatgc taactatgaa gtaatctatg gaagtatagc
     1381 tgaaataatt gtacttatga cctggatgta ttttagtagt tggtctatcg ttattggcta
     1441 tgaggtaaat tcaagattgt attttagaaa gataagacat gaaatgttaa agtaaagtat
     1501 ttaaaataaa agagagtatt tagtattata aatgtaattg aattaataaa gaaatattga
     1561 catttaagtt aaaaagtcta gaatttttct gggctttttt tatttttagt ataacttgac
     1621 aaattttatt taatgggata gttttaattt ataatcatct ttgaaaagat gactattgat
     1681 aagtaaggta taaaattggt aagtatcaaa aaatatacta aataaaaatt atagtcctaa
     1741 tgagttaatt tctgtatttt caaataatgc agtatttagt catgtgtttt tatatgcgcc
     1801 tgtatttcca attaccaata tattagaata agctactgta attagattta gaaaaaagcg
     1861 acataaataa ttataaacaa ataataaatt acataaaatt aaatttattc acaagtatat
     1921 cttataaaat atgttataat taatagaaaa ttgtcatata attaattaag tagtattatt
     1981 ataaataaca ttgaaaggta gtggttagaa tatggatatc cataataaat atgtaaattt
     2041 tataaaaaat atacctgtac cttttttata ttgtagaatt gtaaagagac aagaagatat
     2101 agaatatcga gtagaatata taagtaaggg tatgggcaaa gtattacaat tagaagaagg
     2161 tatttgtgat aaaaatatac tagatgtatt gcctgtattt aagtctaaaa aatatttcaa
     2221 agagttgttc tcaaatgaag tggattgtat aaaaaggtac attccgacac ttaaaaactg
     2281 gataaatata aaaaaacaga taatagggga ttcatacata attttgtatt ttggtaaaat
     2341 tgtatttgat tatagacaga taatcgattc ctttgataaa aaggaaaaag tagcttatat
     2401 taaagacgaa gaaggcatat atatagattg cagtgaaaat ttaataccaa tattaaataa
     2461 caacattaaa acaacgaaag atatctttgg taaaaatgat atagaagtat ggggagagaa
     2521 tacaggaaaa ttatttagag atgattatag aggaggagta tctagtaaaa aaagattttt
     2581 acaaaatctt tttgaatatg aagaaacatt ttttatggtt gaaaaatatt ttttgtatga
     2641 tgaagacgaa cttttaggta ctatagggat aatagataat ataatatata gtggttacag
     2701 taatagaaat tataactcaa aagatttaat gaagatgata gaacactcaa ttccagagaa
     2761 tatgttttat aaagatgtgt atggaaatta tattggattt aattcaggtt ttttgaattt
     2821 agcttgtatg aataaagaag aattattagg gaagaatagt tataaaatat cagaagaaga
     2881 agctttaata aataagatat ttgaaagtga taagggtgta gttgaaaata aaaaagtagt
     2941 tacatttgag ttaaatgtaa gtataaatga tgaaaataag tgtattgaga ttacaaaaag
     3001 gccgtttttt gatagctatg gaagtgttat tggaataatt ggaacagcaa gggatataag
     3061 tagaagaaaa agattagaag aggaaatgga taaaactagg atggaatttt ttgcaaattt
     3121 atcccatgaa cttagaacgc ctataaattt aatatcatct tcattacaag tgatagaaaa
     3181 aaaagaagca gatttaatag aatcaaatga tactttaaaa agaaatttag gtataataaa
     3241 acaaaatgga aatagaatac tgaggttagt aaataatgtt atagacttta caaaaatgca
     3301 atcaggatat ttagatttta aaccagaaga atctgatata attgcattta tagaggagat
     3361 atgtatgtct gtagctgatt ttgcaagcca aaataatata caacttacat ttgatacaga
     3421 aatagaagaa ttttcaatgt tatttgattc agaaaaattg gaaagaataa tattaaattt
     3481 actttctaat ggaattaagt acaataagaa agatggaaaa ataaatatat ttttatatgt
     3541 gaaagataat gtatttaata tgaaaatatc tgatagtgga ataggtatac caaaagaaaa
     3601 aatagataaa atatttaata gatttgaaca gattgacaat gaactttcct acagagttaa
     3661 aggaagtggt attggacttt cattagtgaa atctttggtt gaattgcatg agggaagtat
     3721 atctttaaaa agtcaacttg gtattggaag tgaatttata gtttcattac ctgttagaag
     3781 tgaaaataat attgaaaaat ataatcataa aagagaaatc agcaatgaac taagtaaaaa
     3841 attagaaata gagttttcgg atttataagt ttaaatataa aaaataaaat tttaaaaaag
     3901 tattgacaat tgatttagaa gggtttaaaa taaattacaa cataataaat aaaaaggcga
     3961 tgaataggaa gagtaaatct aattcgatta ccagagagaa gagtccattg gctggaagac
     4021 tttttaaatt agagggattg aaaactacct aggagctact ttctgaagtc atctgcatta
     4081 gtagatttta gtaagaatag tcgttagctg cgttaaagtg taaagtgagt tttcatgata
     4141 tatgaaaact aaattaggtg gaaccgcggg aataatactc tcgtccttat gttataagga
     4201 tgagagtttt ttgttgttta aaaatataaa cagtaatcat aataattata gaaaatataa
     4261 aagcgatgaa taggaagagt aaatttaatt cgactatcag agaggaaagt tcacaggctg
     4321 aaagactttc taagtttgag aaattgaaaa ctacctagga gctgtggtct gaagttactt
     4381 taaaaagttt tagtaagact aaccgttaac tgcgttaaag tgtaaagtgg attttatcaa
     4441 taaatgataa aatcaaattg ggtggaaccg cgagtataga ctcgtccctt tatataggga
     4501 tgagtctatt tttattgcaa taaaagattg aaaataaaaa aaaatcggaa aattataaat
     4561 ttaggaggaa aataaaatgg aaaataaagt gaaaattgga gtactaggat atggagttgt
     4621 aggaagtgga cttatagata taatagataa taataaagaa aaaagaagta tagaaatagt
     4681 tggaatttta gttaataatt tggaaaaaca taaggataaa aaatattcta atataataac
     4741 taataatata gatgatatat ttaataaaga tatagatata cttgttgaag tcatgggtgg
     4801 tttagaacca tcattatctt atataaaaaa agcattaaac aataaaattc atgttgttac
     4861 agcaaataaa gatttgttag ctgaatgtgg agatgagctt gcaaaattag caagtgaaaa
     4921 taaagtaagt attaaatttg aagcttcagt cgcaggaggt attccagtat taaaaccaat
     4981 aatagaatca ctagaaggaa ataatataga tagtataaat gcaattttaa atggaactac
     5041 taattttata ttatctaaaa tgtatgatga gaatctatct tatgatatgg cattaagaca
     5101 agctcaagaa ttaggttttg ctgaagcaaa tccagagtca gatgtattag ggtatgatgc
     5161 tgctagaaaa ctatcaatat tatcaactct agcttatgac aatagagtat attggaagga
     5221 tgtgtattta gaaggaatta cagatataga tgaaaaagat attgaatatg caaaaaaatt
     5281 aaattgtaaa ataaaattga ttggtcaaag taagtatgaa aatgataaag taagtgcttt
     5341 tgttagacct gttttagtag aaaaagataa tatccttgct agaatagaca atgagttcaa
     5401 tgctgtcata gtgaatggag attcagtagg agaagtttct tttgtaggta aaggagctgg
     5461 aagtttagca acaggaagtg cggtttattc agatgttata gatataatag ataatagagt
     5521 ttcaagtata gattctttta ctaaagataa aatacaagtt aataaaatag ttagagaaaa
     5581 gtgtggagca cttcttagat ttaaaaaatg taataaagat gaaattctaa atatagttga
     5641 gaattgttta gtaaaattcg atattttaaa tgatgatgat gaacttgcta ttatggttta
     5701 tgctgattct gaatatgaaa taaacaacag tttatgtcta ataaaagata aaggatattg
     5761 cgaaaaaatg aataaaatgt tgaaaataag ttaaaaaaga aaaaataagt ttatatgtaa
     5821 catattttga cgattttaat actatatagt atgagatgaa acaaaacatc tcataaaaaa
     5881 ttaaaattca agggagagat atgttatatg gaaaataaaa aatatgcaaa tggtggttat
     5941 tcagaagact ggtatgaaag aggagaatct acagctaaat ggttccagaa tgatagagaa
     6001 gaatatgaaa gagcagcata cgatgaagat agagaaagaa gaggctcaaa ctgtggatgt
     6061 tcagattcag gaggaaatag acctagaaac tgtgaaagat ttagaagaga agctgagata
     6121 agagaaagag aagcaagaga agcattctgt gaatcttcag agagaaagaa agaagcacta
     6181 gcatatgaat gtgaagctag aaaattatgg gaagaagcag aaaaatactg ggatgaatat
     6241 tcaaaataca actataaagg aatcgaatat ttagcagaag ctgctagatt atttgatgaa
     6301 ggtatggaat gtgaagctag aagaaatgga aataatggag gaaacaataa taattgttgc
     6361 cataaatgcc ataaatgtaa ttgtaactgc tgtagaaaat aaaaaaattc attgacaaat
     6421 caatagttaa aatatatcat attatgtata ataaagagaa aattcagaaa aaaataatga
     6481 ttagaataaa tttaagtata tttattagac aaatagtgct cagttagtga ggtgagtaca
     6541 atatgattaa actcccatcc tctagctttt gatagagggt gggagttttt atttattgat
     6601 atatcttaaa tgaggataat attgtaaaat aaatgagtat gagattgtaa aattaggagg
     6661 taaaaaatga aaagtattaa aacatcaagt atgaaagaaa aggtagatga aaagatatta
     6721 agagagcaag taagttctat aatagaagat ataagaaata ataaggatgt tgcacttaaa
     6781 aaatataatg agaagtttga tagaaataca agagatgaat ttagaattac taaggaagaa
     6841 ataaaggaag cttataagca tgtagatgac gaatttataa ataacttaaa gattgctgcc
     6901 aaaaatataa gagaatttgc taaagctcaa aaactaagtt ttgaaaaccc ttttgaaaaa
     6961 gaaatttatc caggtgtaat cttaggtcaa actaatattc caatagagtc atgtcttgca
     7021 tatgttccag gaggtcagta tccattgttt tcaacagctc ttatgcttat aatacctgca
     7081 aaagtagcag gagtaaaaag aatagtggca tgttcaccaa ctatgaaaaa tactgaaaag
     7141 ataaatccta aaacattggt agctatggat atagcaggtg ctgatgaaat atatgcaact
     7201 ggaggagttc aagctatagc agcatttaca tatggaacag aaaagattaa tccagttgac
     7261 ataattgtag gaccaggaaa taagtttgta actgaagcta aaagacaatg ttatggacaa
     7321 gtaggaattg attttgtcgc tgggccaagt gaagttctta taatagcgga tgaaacttca
     7381 aatccagttt atattgcagc tgatttatta gcacaatgtg agcatgattt aaatgcaaga
     7441 ggaatattat tgacaaatag cttggaagta gcacaaaaag ttgaaaaaaa tatagaaagt
     7501 atgctaaaag atttgccaac aaaggacata gcatatagtt catgggaaaa taatggagag
     7561 ataatattgg ttgatgatat ggaagaagcc attaaaataa gtaattttta tgcaccagaa
     7621 catttagaag ttgcagtaaa tgagtataat gatatttgtg atagattaac taactatggt
     7681 tctttattta ttggaaatct ttctgctgag gtgtttggtg actatgtttc aggtaccaat
     7741 catacattac caacacttaa agcatcaaga tatactggtg gtgtttgggt tggaactttt
     7801 atcaaaactt gtacaaagca gatatttaat gaaaaagcta tacaatcatt agcacctgta
     7861 gctgaaaagc tggcaaaaga agagggatta tatgcacatg caaaggctgc tgaggttagg
     7921 tttaaaaagt agtaaaaaat tttattttgg cagtcgagat ttagtattag actgagataa
     7981 agaaaacgct gagaacctga ataaatacta tattttgtgg gcctcggcgt ttcttttata
     8041 tctgtatata ataggataat actacctgtg tgtttaaatg ctaacttaat atttcttttc
     8101 taaataatgt ttatgaaata gttaatattt ttagttaata cttgaaaaaa acttttgcta
     8161 actgtgcaaa ttcatctgca tttgtcaata tagcagggtg tcctccctga ctaaaaatgt
     8221 gaatacttgc ttttgaaata aaagttgcca tttgcttgta ctcttcttca agattatgac
     8281 gacacatttc gtcttcctta cttcctgtaa ataaaattgg catttctaat ttacacaatt
     8341 ctttatgaaa taaagggcgt ttcttgtttg cacattgcaa tagagctttt gtatcaagat
     8401 taacaacatt ctcccaatct tttccatgac accattcata gaattttcgt gcttgaatat
     8461 cttgctttga tttttccctt ccagtgatta gattatctat gaagttatca ttaagagtac
     8521 gtccatcaaa gctatctgct ataattgctt caactaaatc gggacgttct aatccagtat
     8581 taacagctgc ccatgctcct ccacttgttc caattaagcc aactttacca caattcaaat
     8641 gttcaatcag tgctattgtc tgtaaggctt catcatgcca tatgtcagga gaaaattgag
     8701 aaactctatc agactgtcca ttacctaaaa aatcaattaa gatacatcta aaattttcta
     8761 catataatgg cacaagtgct ttaaacatat tagaagaacc agtattacca tgcaggaaaa
     8821 ttatcggttt accttgtcca tattctttat aaaaacaaga attaccttga tactgaaaat
     8881 atgacatata ttttcctcct ttttatttgt taagttaatt ataacaatat aggtatgaat
     8941 gaaaaacctc atgttctttc ttagaataaa aatgtgccac gcataaaacg tgacacattt
     9001 ctaattataa attttgctgt ttaagtattc tttgtatgct tttctctgtc aaaaagtatt
     9061 tctctgataa ctgcttgata ctcattccat tttggaaatt tatacagatt tgagaattac
     9121 gagaagctaa gacttgtttg gtgtttgtgt tttcacccca atgctttttg tgctcttgct
     9181 ttttgggaat ataaatcact ttaccatcta tatattcttg cacttgctca atcagttctt
     9241 taggcagtaa gtcaatcgaa ttttgatagc tcattttgca cctcctaaat aattactctt
     9301 taggattagc aaaggctact gctactcaca attaaaaata aaatttttta ttatgtacaa
     9361 tagcctttgc tatgcacaag ttccaattcc cataaactaa atactccttt ctggtttgaa
     9421 aaattcatct gaacattgat ttcgatttta agatagagtt caatgacaaa tttacatttt
     9481 tattatatat attcagatag ataattgcaa ctgtctttgt atgaggatat tttattatag
     9541 tcaatactat aatatatatt tatatcatca aactaatatg aacagttgaa aggaatatga
     9601 acaataaaac ttagtcagga agaataatta tgaagcagac aattgagaaa tttgatttta
     9661 aagttttatt gaaaatttat tgacaaattt aacttgtaaa tattatacta aacttaataa
     9721 ataaagttta taaagtaagg tgttgtctat gaaaaatata gttggaaaac cgcaaacgat
     9781 aaaaaaagta aatgaagatt taataaaaaa cattataaaa aatgaaggtc ctataacaaa
     9841 accagaaatt tcaagcatta ctgaattgag tttagctaca gttaataaga ttgtggagca
     9901 attagctttg aaaaatgaag ttaaagtaag tggactaagt gaatctactg gaggaagaag
     9961 agctcaatta tttgaaataa atgcaaactt agaacacata atagctttat attattatag
    10021 aaattgttgt ataggggtag ttgccaattt attaggtgaa ataatttatc aagaagaatt
    10081 taatataaga atggatacat atgaaaacgt taaccaagat actttttttg ttatagatac
    10141 tctaataaaa aaggaaaaag gaaacaatat aagggctatt ggaattggtg tcccaggagt
    10201 tgtaaaaaaa ggtgtagtta aaaatatacc aactataaaa agttgggaag gtataaattt
    10261 agaaaattta ataaagagta agtacaatac taaagtgttt atagaaaatg atacaaatat
    10321 tactactatt gggatttatc aaaaacagta taaagacaaa tataaaaata tggctcttat
    10381 gtatttagaa gatggtattg gttcaggtat agttataaat ggtgaattgt atttaggttc
    10441 tacaaatttt gctggagaat taagttatct aaatatagaa tttggtaagt ataactctaa
    10501 caacttaaat cttgaagatt atattatgag tttaagaaaa agatatttag aaagtaaaga
    10561 taataaattt aagaaagaaa ttttatctat aatatcaaac tttataagga ctcttgtatg
    10621 tgtcctaaat ccacaagcta tgattataca gtgtagtata ttaaccaaga gtgacatgga
    10681 gtttatagaa aaagatgtaa gtaataatgt tggagaagaa aattttccta aattaataaa
    10741 agtagagtca ttgagagaaa gaagtataaa tggagtaatt agtatgtgtt taaaagaaac
    10801 tgactatcaa tattccttat ctaataaaaa aggaggatat taatgtgaaa tatttggttt
    10861 caatagatgg agggggaaca aaaactaaat tttgtgtaag tgacttagat ggaaacatac
    10921 ttaaagaaca taccactggt tcaactaatt ataagtcagt tggaattaaa aaaacatatg
    10981 aaaatattaa taatggactt aaaaaaatat taaaagattt atatatcgat tatgacgata
    11041 ttgagtacac tgtatttggt atatctggtt gtgattctcc taatgattat aagattataa
    11101 tggatgaaat attaaaaatt gggattaaca aagaaaaaat atatctggca aatgatgcag
    11161 ttttagcttt ttatgcacaa gctgattccc ctggtttagt aattgtggct gggacaggtt
    11221 caattatact gggaattaaa gaagatggag aaatatatag agttggtgga tgggggtata
    11281 attttagtga tttgggttct ggatatgata tagggagaaa attattaaag aaagtattgc
    11341 tttattgtga tgaatgtcat gagtatagtg atttatttag ttgtgtcttg gatttctttg
    11401 gagcaaatag ttttgaacaa cttgcatata tgattactga tataaacaat aatgttgaaa
    11461 tagcaaacct agcttcatta gtgattgatt gtgccgaaca aggtgataaa ttagctattg
    11521 aaatattaag agaatcatca actgaactat ctaaattagc tcaagtaata ttgagaaaaa
    11581 tatctaatcc aatgaaaata gataaaagcg aaataaatat agttttgtct ggtggtacat
    11641 taaataaaac tgtatatgct caaatgttaa tgaataattt aaaaacagaa aatacagata
    11701 aaaatttaaa atttatatct caagaaaatc agccagtata tggggggata agacttgcta
    11761 tggcattagc taataggaga gttaagcatg gatagaaaaa aggtaatagt acaaattttt
    11821 actggaggtt ttaaggctaa agaaataaag tttaatgata taaaatgtaa actactagaa
    11881 ttattggact gtattgatat agaaaaggtt atcatgggat ggagtgtaga tgaagaatta
    11941 tatgtagaaa ctaaaaaact attaaataaa tataatgttg aactatatct ttggttgcca
    12001 tgtttttctg aaataggatt acttgaaaaa agtaatttac taattgatta tatggggaag
    12061 gaagtcaagg gctatgcttt acaagaggat gaaaattttg aattttattg cccaaataat
    12121 aaggaaaata taaaaaatat aaagaaaata tatagtactt attttagtga tattaaattt
    12181 gatggaattt ttttagataa aataaggtat ggttcttttt cgaataaact aaatggtgtt
    12241 tttaattgtt tttgtaaaga ttgtttaaat atatatgaaa aaaataatat tgataaaaag
    12301 tcgttaatga gagaaatgaa taatgtgtct aagggagtta tagataatga agatactcca
    12361 tctggtataa cagaatacat taatggaaaa tacaaattta aaaataaaat atgggaagat
    12421 ttttttgaaa caaaaaatca aaatatatac aagtcattat tagagataag tagttatttt
    12481 aaaagtaagg gattgaaaat aggtatagat actttttctc cttttatttc ttactttaca
    12541 ggtcaagatt taaaaaaatt acaagaagta gcagatttta taaaaccaat gatgtataga
    12601 actacaaaag ctccagctgg tttaccattt gaatttgaaa ggtttattga tgaatccata
    12661 cataagaata aagagattgg aaaaagaaat ttcttagata ttttaggaat taaagagtat
    12721 gaaggagatg tatatcctat agaatttgta ctagaggaaa tcgattttat gacaaggaat
    12781 cttaataaag atatatatgt aggagttgaa ataaataaaa aggaaattat agctcctgtt
    12841 acacctaagt acataagtga aaatatttca aatataaata agacaacagt aaaaggatat
    12901 gttttatctt gggatttgtt atcagcacca aaagaaaata ttgatgaaat tataaaatgg
    12961 tttcggaatt agtgaggtga tatagtgagt aagatattag aaatgcaaca aatatataaa
    13021 agttttggaa aggtagatgt tttaaaaggg ataaattttg agttagaaaa gggagaagta
    13081 agagctcttt tgggagctaa tggagcagga aaatctacac ttattaaaat tttaggagga
    13141 gttcattctc aaactaaagg taaagtaatt ttgactggta atgaaataat gtttaaaaat
    13201 tcacatcatt ctaaagaaaa tggtattagt ataatttatc aagaattaag cttgatacca
    13261 acgcttacag tacttgaaaa tatttttctt ggtagagaaa ttttaaaagg aagtgtgttt
    13321 ttaaacaaga aagacatgct tgaggaatat atgaaaattt gtgaggattt tgattttaat
    13381 atagacccac atacagtagt ttctaattta agtgtagcaa accaacaaat ggttgagata
    13441 atgaaagctg tttcatgtaa ttctgaaatt atcataatgg atgaacctac aacatcactt
    13501 actaataatg aaaaagatgg gctatttaaa ataataagta gactaaagtc taaaggtaaa
    13561 acaatagtct atatttctca catattagag gaagttttta aagtatgtga tagtgcaagt
    13621 ataatgaaaa atggaattat gattggaact tatgatgtag aggatttaac taaattaaag
    13681 ataactcaac ttatgacagg aaatgataat cacaaactta ttgagaagaa ggattataca
    13741 tttgcaaagt atgaagagcc accagtacta gaagtaaaga atataagctg gtctaacaaa
    13801 ataaaaaatg tttcatttaa agtttataga ggcgaagttg taggtcttgc tggtcttgta
    13861 ggttcaagaa gaactgaaat tataaatttg atatttggta tagataataa agatagtgga
    13921 gacattttta tcaatggtaa aaaagtagac ataacttctc ctaaggttgc tataaaaaac
    13981 aaaataggat tgattcctga agatagaaaa aatctaggat taattttaga acaagctata
    14041 tacacaaact catcatcagt acaaattgat aaattcaaga aatttttgtt tatagataga
    14101 aataaagaaa ttgagttttc taaaaaaggt gttaaggaat taggaataaa agttagtgat
    14161 gtaaagcaag aagtaaaaga gcttagtggt ggaaatcaac aaaaagtagt agtttctaag
    14221 tggttagaga aagatttaga tttaataata tatgatgagc cgacaaaggg aatagatatt
    14281 gctgcaaaag aagatatttt caatacaatc aaagaatttt caagtgaagg aataggagta
    14341 atatttatat cttctgattt agaagaagta ataagagtaa gtgatagaat tttagtaact
    14401 agagaaggtt ctattgtaga tgatttaaaa aatgagaata tacaggttca agatataatg
    14461 aacagtatat ttaatgtata aaaaatgaaa atggaggact gagatgaaga aagcttttga
    14521 gaagttaaat ttaagagatt atggagtatt attaggtttt ttagctttat gtatagctat
    14581 aagtattgca actccatcat ttttaggtaa acaaaatata cttaatcttt tacgacaatc
    14641 ttctataatt ggaataattt cagcaggtat gacttttgtt ataatatctg gaaactttga
    14701 tatatcagtt ggagcagtag cagctcttgc aggtgctata acaatgaaat ttgcgactgg
    14761 tggaactaat ttgtttgcat ccatgctctt aggtgtagcg atttgtgcag taataggatt
    14821 aatcaatggt atagtagttg caaagataaa tgtaccatca ttaatagcaa ctatggctat
    14881 ggtcactatt gtaagaggat tgctgttgat gcttacagga ggatatccaa ttactgaaaa
    14941 tataccaata cttgattaca ttggaaatgg atatttgttt aatataccaa taccagtagt
    15001 aattttcttt atagtggtgt tagtttcatt tattgtactt aataaaacta agtttggtag
    15061 atatgtttat tctgtaggag ggaatcaaga agcaagtaaa cttaatggta taaatgtaga
    15121 ttctcacaaa gtgaaagtgt ttattattaa tgctgtactt gcaggaatag ctggaatcgt
    15181 actaacagga agacttggga ctgcaactgc tattgcaggt gaaggatatg atatggatgc
    15241 aattgcatct gtagtaatag gaggtacatc agtggcagga ggttctggtt ctgtacttaa
    15301 aactgttata ggagtattac ttatgagtgt tataaacaac agttttaatc ttcttggaat
    15361 agatgtatat ttccagtaca tatttaaagg attaataatt ttagcagcag ttggatttga
    15421 ttcttatagc aagaaaaaac ttgcttcaag ataaaaaata agggggattt ttttatgaaa
    15481 aaattaatga aacatttagc attgttgtta tcacttgtta tgatatttgg tttagttggt
    15541 tgttcaaatg gtggtgacaa agaaaaaaat ggagaaaaga aaatagcagt attactacca
    15601 ggttcaacag gttactttgt agcaactaaa caaggtatag atgcaaaatc taaagaatta
    15661 ggtgtaagcg tggaatatgc agatgctcaa tgggatgcta gtaaacaatt atcacaagct
    15721 gaagatttta tggctaaagg ggtagacatg attgcattat gtggagttga ttctgctgta
    15781 agtgagagaa tagttaaagc tgctaatgat tcagacgtcc ctatagtagc atttacaaat
    15841 tctataggaa gtaacccaac tggagaattt aaaggtttag ttacatatat aggacaaaat
    15901 gaggaggaaa caggagcact aactggaaaa attgctaaaa acttattagg agaaactggt
    15961 gggaaagctg tattaataga aggagtacca ggaactactc cacaagtatt aagaaaaaaa
    16021 ggtcttgaaa aagaattaaa agatagtaat atagaaatag tttataatca aacttcaaat
    16081 tgggagaaag agcaagcttt aaaagtaaca gaagatttaa ttcaaaagaa aactgatttt
    16141 aatgttataa tatgtcaaga tgataattct gctacaggtg caggtcaagc tttaaaagat
    16201 gctggtctta aggataaagt taaagtcata ggtttgggtg gaagtaaaga tggattaaaa
    16261 gctataactg atgggttaat agatggtaca acttatatgt ctgctgtaga agaaggtgga
    16321 ttagtcatag aaaaggcatc taaattcctt aaaggtgaaa aaatagaacc tgttacacaa
    16381 attaaacaag ttgaagttaa caaagataat atttcagagt tcaagggtga atggtaaatt
    16441 gtcatagata ataaataaga tttttagagt tactttagaa actatataaa gtaactcttt
    16501 ttttaattta atttagtgat aaattattga atagtatttc taaacaaata aaaaattaat
    16561 ataaacaaag acttaattat actaaattat aagtaaaact aatataaact tatggaaaaa
    16621 aggaaaacgt ctaaagtatt ggaattacta tagtgtaggt gtggtattaa aaaaacataa
    16681 aaaaacataa aaaaattgtt gaaaaaaata tagaaatttt gtataattaa agatttgaaa
    16741 agttcggtat acaaagccaa ttcttttgta tataatatat ataaattcag aaaaaggaag
    16801 ggatatgtat ggccaggcgg ttatttttag aaaggattgg acttatattt ttaggttcag
    16861 caatattagc atttggagtt tataattttt attatttaaa caacataaca gaaggtggag
    16921 tgttaggtat tttactactg ttaaaaaatt tattcaatat acaaccagca atagcaaatg
    16981 ttgtaataga tggtttacta cttttagtag gatataaatt tttcggtaaa aaattcctta
    17041 tatattcaat agtagcttct ataacttttt cagtgttata tgacttattt gaagctatag
    17101 gacctctagt accacaatca caaaatatgc ttttatcaac tattttggca ggtgtaactg
    17161 ttggaactgg tgtaggtata gtagtaaaag caggttgtgc atcaggagga gacgatgctt
    17221 tagcattagt aatttctaag actacatctt tgaacatagg gcaagtttat ttagcaacag
    17281 atgttattgt attgttgtta tcattatttt atctatcagc gtttgatatc ttttattctt
    17341 tgattgctgt tacaattagt ggcaaggtaa ttgattttat ttattatcat ggtaaaagtt
    17401 tggatatgga tataagtaat gatatagttc cagaatgtta atataaatta aataacttta
    17461 gattttatta ggggtggcag atttgccacc ttttttgtgt ctaaatcttt atagaaagta
    17521 taatcccatc taggtttttg caaatctcaa atatagaatt atgttggaat gactgggaat
    17581 acgattgcta gatgtgcatt ttaaaaaccc aattttatat ttatgaaata ctaatcatta
    17641 atggatgttt cttaatccta tctttatacc taaaatgata agataaatga agtagcaata
    17701 caaaactaaa atttgtaaaa gagattaaat taaatggagg agctaaaatg ttggatatag
    17761 ttatgatagc attgttattc ataggatttg tttccatgaa gttacttgct gattggtgtg
    17821 ggaaacaaac tgagaaaaaa taatgtaatg tatggaggga ttacctatgt ggtttctggc
    17881 agttgtaatt ataatgttaa taatatattt ggtatatgcg cttattaatc ctgaaaaatt
    17941 ttaaacagta taaggctaat aagtaagttg aattgggggg aacaattttt atggtgcaga
    18001 tagtaatagt gttagcgatt tttatgattt tagtaatacc tatgggcaag tatttatatc
    18061 atatagctac taatcaaaaa acatttggag atagactatt tgataaagtg gacaatttta
    18121 tatataaggt atgtagtata gataagaaaa aagaaatgaa ctggaaacag tatgcattag
    18181 ctttattatt tacaaatgca gttatggtat ttataggata tatcatactt agaactcaaa
    18241 gtatgcatat ttttaatcca agtggaatta aaagtatgga acaaggacta tcatttaata
    18301 caataataag ttttatgaca aatacaaatc ttcaacatta ttcaggtgaa tcaggacttt
    18361 catactttag tcagatgaca gtgataatat atatgatgtt tacatcagca gcaacaggat
    18421 atgcggctgc tatggcattt gtaagaggtc tagtcggtaa aaagaaaaca ttgggaaatt
    18481 tttatgtaga tttaattagg ataacaacaa gagtactttt accaggagct ttaattattg
    18541 ggcttatatt agttacacaa ggagtaccac aaacttttgc tgggacagaa acagtgacta
    18601 ctatagaagg aaagcttcaa gatatagcta gaggtcctgt ggcagctctt gaatcaataa
    18661 aacatttagg aacaaatggt ggtggattct ttggttcaaa ttcatcacac ccatttgaaa
    18721 atccaacaat aatatcaaat atagtagaaa tattatctat gatgatatta ccaggagctt
    18781 gtgttgttgc attcggtcac atgataaaga ataaaaaaca aggttgggtt gtgtttggcg
    18841 caatgtcaat catattttta ataggtctag tagtgtgctt taaagcagaa agtgcaggaa
    18901 accctatttt atcacaatta gggcttaatc aaagtatggg aagtatggaa ggtaaggaag
    18961 ttagatttgg tatagcacaa tcatcattat tcacaacagt gactacttcc tttacgacag
    19021 gtacagttaa taatatgcat gatacactga ctcctttagg aggattagta ccacttttaa
    19081 atatgatgct aaatgtagta tttggtggta aaggcgtagg tctgatgaat atgttgatgt
    19141 acgctataat agcagtattc ttatgtggat taatggtagg aagaacacca gagtttttaa
    19201 ctaagaaaat cgaaggaaaa gaaatgaagc ttattgcttt acttattata ttacatccac
    19261 ttttaatact aatgttctct ggtctttcag tagcaatacc agcagggctt gaaggaattt
    19321 caaatcctgg attccatgga ttatcacagg tattgtatga gtttgcatct tcagcagcca
    19381 ataatggttc aggatttgaa ggtctaggag ataacactat gttttggaat ataacaacag
    19441 gtatagttat gttctttgga agatatgtgt ctattatagt actactagca atatcaagtt
    19501 tgttagcatc taaaaaagct gtaaatgaaa gtataggaac acttagaacg gataatttta
    19561 catttacaat agttttagta cttgttgttt taattgttgg tgctttaaca tttttcccag
    19621 ccttagcgct tggacctatt tcggagcatc tagtgttgtg gcactaaaaa attgggaggg
    19681 agtaaagttt tatggaaact aaaaaaacaa agtttattac aaaagatatt ttcaaaactt
    19741 ccattataga agcttttaaa aaattaaatc ctaaatatat gatgaaaaat ccagttatgt
    19801 ttgttgtaga aattggattt atagtaacat tgatacttac aatagtacct agtttgtttg
    19861 gtgatgaagg caataattta agagcttata atgggattgt aacaataatc ttatttataa
    19921 cagtcctttt tgcaaatttt gctgaagctg tagctgaggg aagaggtaaa gctcaagcag
    19981 atacacttaa gaaaactaga aaagatacag tagcaagggt tatagatacg ttaggaaatg
    20041 aaaaaatgat aagtgcaagt gagctaaaac ttggtgatat tgttttagta aatgttggag
    20101 aagttatccc aaatgatgga gaagttatag agggcatagc ttctgtggat gagtcagcaa
    20161 taactggaga atctgcacct gtcatgagag aatcaggtgg agatttcgca tctgttacag
    20221 gtggtactac agttgtaagt gactggttaa agataagaat aacaacaaaa ccaggagaat
    20281 cgttcttaga taaaatgata gctcttgtag aaggtgcttc aagacaaaaa acgccaaatg
    20341 agattgcttt aaatactttg ttagtagggt taacacttat atttatggtt gttttaatat
    20401 ctctttatcc aatggcaaac tatgcaggtg taaagattcc aatttctact atgatagctc
    20461 ttttggtatg tttaatacca actactatag gtggattgtt gtcagctatt ggtattgcag
    20521 gtatggatag agtaactaga tttaatgtaa ttgcaatgtc aggaaaagca gtagaagctt
    20581 gtggagacgt tgatactatg attttggata agacaggtac gattacttat gggaatagac
    20641 ttgcatcaga atttatagca gttggaaatg cagattcaaa agatttaata aattattctg
    20701 ttatgtgttc attaaaagat gatacaccag aaggaaaatc tatagttgat ttaggtatga
    20761 aattaggttc aacaggtaag acaaaagagg cagaggaagc tgagtttgta gaattttctg
    20821 cacaaactag aatgagtggt attaacttag ctgatggtag agctataaga aaaggtgctt
    20881 atgatagcat aataaaaaga gtaaaagaat ctggtggtac agtgccagaa gatttagaag
    20941 aaaatgtaaa tcgagtagct aaactaggag gaacaccttt agtagtgtgt gtagataatg
    21001 agatatatgg aattatctac ttaaaagata cagtaaaatc agggcttgta gaaagatttg
    21061 ctagacttcg agagatagga ataaagacaa taatgtgtac aggggataat cccttgacag
    21121 cagctacaat tgcaagagaa gctggagtag atggatttat tgcagaatgt aaaccagaag
    21181 ataaaataga tgctataaaa aaagaacaat tagagggtaa aatagttgca atgacaggtg
    21241 atggaacaaa tgatgctcct gcacttgctc aggcagatgt aggtcttgca atgaatagtg
    21301 gtacaacatc tgcaaaagaa gcagcaaata tggtggattt agattcagac ccaactaaga
    21361 tattggaagt tgttgaaata ggaaaacagc ttttaataac tcgtggggct ttgacaacat
    21421 ttagtatagc aaatgatgtt gcaaaatatt ttgcgattat accggctata tttactcttg
    21481 caatacctca aatggagata ttaaatgtaa tgaaactttc tacatcattt agtgcgatat
    21541 tatcagcttt aatatttaat gctataatta tcccatgttt aatacctctt gcaatgaagg
    21601 gagtaaaata tagacctatg aaatcacaag ctatgttaat gagaaatatg cttatatttg
    21661 gtcttggtgg agttatagta ccatttttag gaataaaatt aattgattta ttgattacac
    21721 ctttagtaat gattcttaat ttaggttaat aaatggagga tataaaaatg aaaacattaa
    21781 agccagcatt acttgtaagt attgtattgt tagttgtttg tggacttgta tatccacttg
    21841 ttttgacagg tgtaagccaa gttgcattta aagataaagc caatggaagt atgatagaag
    21901 taaatggtgt aaaagtaggt tcagaactta ttggtcaaag ctttacagat gcgagattct
    21961 ttaagggaag agtatcatca gtaaactata atacttatac aaaagaagat ttagtaccag
    22021 ataaagatgg aaatgcatct tatggaggtg tatcatcagg gtcttttaat tatggagcta
    22081 caaatccaga acttcatgat agagtacaaa aagatataga aaagttttta aaggataatc
    22141 caactgtcaa aagagaggat ataccaacag atttacttac agcatcaggt tcaggacttg
    22201 accctaatat aagtccagcc tctgcaaaaa ttcagatacc agctatagca aaagcgtctg
    22261 gaatatcaga aagtaaatta caaaaaattg tagatgataa tacttccaaa aaactttttg
    22321 gagttttagg tgaagataga gttaatgtac taaaggttaa tatagaggtc gcaaaaatat
    22381 taggtcttat atagtatttt gtggaatttt agtaacaaaa tattactcaa gatttaattt
    22441 aaaggttaaa ttttaaagag tgaaaatcaa taatgatttt cactttttta tttgaatatt
    22501 actaatattt ggaataaagt gattgtaagt ataaaatcca aaaaaaggtt gcattatcaa
    22561 aatgataata ctataatata tttatcgtta tcaatttgat aatagatttg gacatatagg
    22621 aggaaatagt atgttatata ataacgcatt agagttgata ggaaacacac ctgtagtaag
    22681 gctgaataat ttaggctgta ctaatgggtg ttctaacata tatgtaaaat tagaaaaagt
    22741 aaatccagca ggaagtataa aagatagagc tgtttatgaa atgttagagg gcttagagca
    22801 aagaggagaa ttaaaaaagg gagatgtact agttgaagca actagtggaa atactggtat
    22861 tgcattatct atgataggaa aattaaaagg atatgaagta gtaatcgtaa tgcccgaaac
    22921 aatgagcgtt gagagaagag atttaatgaa ggcttatgga gcaaatttgg tgcttacaga
    22981 tggtaaagct ggaatggcag gttccataga aaaagcaaat gaacttttaa aagaaaatcc
    23041 aaactataaa agcttaaaac aatttgaaaa taaagataat ccaaatgcac attataaaac
    23101 aacagcagtt gaaattatga atgatgtcaa agatttagat atattcgtat gtggagttgg
    23161 aacaggtggt actttaatag gaactgcaag atatttaaaa gaacaaaatc caaacataag
    23221 agtaattgca cttgaaccaa aaaaatctcc agctatatca gaaaacaaga ctggacctca
    23281 taaaatacaa ggaataggtg ctaactttgt tcctgaaaat tatgattcta gtgtagttga
    23341 tgaagtaata ttagtagatg atgaagatgc atttgataca gttaaattac ttgcagcaaa
    23401 agagggaatt ttagtaggga tatcttctgg agcaaatata tttggtgcta tagagatggc
    23461 aaaaaagtat ccagataaaa agatagttac agtagctcca gatggagttg ataagtatat
    23521 gtctatggga atattttaat aattttattt gagggagtga atactttgtt taagaaaata
    23581 aataaagata ttgaatatat aatgaagaat gatccagcag ctaggtctaa gatagaagtt
    23641 tttttacttt atccatcagt tcatgcaatg ataatgcata ggatggctca tgctctttat
    23701 aagaagaaga agctttttac agcaagatta atatcccaga tatccagatt tatgactggc
    23761 atagaaattc atcctggtgc taaaatgggg gaaggtatat tgatagacca tggaatgggt
    23821 gttgtaattg gagagacagc tgaagtaggt aatagggtga caatatatca aggagctact
    23881 ttaggagcta caggaaaaga tactggaaag agacatccaa ctgttggtga tgatgtatta
    23941 attggtgctg gaacaaaaat actaggacct ttaaatatag gttcaaattc aaaaattggt
    24001 gcaaattctg ttgtcgttaa ggatgtacct aatggtgcaa cagttgttgg tataccagca
    24061 aaaatagtaa aaataagaaa cctagaacct gtaaaaaaga ataaaaaaga agtgtcctat
    24121 gagtatgatg aattagataa tgtatattat atataggatt tatataacta taaaataaac
    24181 tttataaaaa ttattttaaa aggtattttt ttcaaatttg aggacataat aaaaaagaac
    24241 tgaatgttac agttttggtt catcaatctt tgataaacaa gattttaaat ttgtagcgtc
    24301 taggaggatt ttattatgaa agcaaatgtt aatcaagata cttgtatagg ttgtggatta
    24361 tgtccatcaa tatgtccaga agtatttgat atgaaggatg atgggaaatc acatgttata
    24421 gttgatgaag taccaagtga tgctgaagaa tcagcagcag aagctagaga aagctgtcca
    24481 gttgatgcaa tagatgtgca ttaataaaat aaatgcctat aaaaattgaa ttttcaattt
    24541 ttataggcat ttgtctttta ttcagttagt atttcaaccc ctgtttctgt aactaaaacc
    24601 atacattccc attgagcaga taatttacca tcttttgtat aaatagtcca attattttct
    24661 tcatcaacaa aaatttcgtg agttccttca tttaccatag gttctattgt gaatatcatc
    24721 ccaggaacca atagcatctc agtccctttt ttagatacat aacttacaaa agggtcttca
    24781 tgaaagctta atccaactcc atgaccacct atttctctaa caacagagta tccatttttc
    24841 tttgcataag agttgatagc atcacctata tctcctaaaa atccccatgg tttggcaact
    24901 tcaagacctt tttggacaca ttcctttgta acttgaacta atttttttgc attttcatta
    24961 acattaccta tcataaacat tcttgaagca tctgaatagt atccattaaa aatagtagaa
    25021 acatctacat ttataatatc tccatctttt aagataatgt ccttgcttgg aataccatga
    25081 caaacttcat tatttactga tgtacataca cttttaggaa atccattata gtctagtgga
    25141 gcagggatag ctccattttg tagtgtaaag tcatgaacca acttattcat atcttctgta
    25201 gacattccaa tttcgatttt atctgaaata taatccaaaa tttttgtgtt tattacagca
    25261 ctttttttta tttcctcaat ttgactttca ttttttataa tatcatgagt tggtacaata
    25321 tgacctaact tagcaaatga atctatttta ttatccatat ccatatgaca ttttttatat
    25381 tttttagtac ttccacacca acagctatca tttctattta acttcataac ttttcatcct
    25441 tcttttatta tttatttaaa ttatgggtta cataaactat tttaaaacat aaccattata
    25501 aatattataa cataaatact aagaaatcct ttatagatag tgatttattc aaagtataaa
    25561 ttattatatg gttattgttg atgaaaagag gatagttaaa tgtattattg gatattagcg
    25621 cacttaatag tttagttcaa gtttttcaag aaaatcatat gttagaggac aattttcctt
    25681 taaagtattt tttgaatctt catcaagata ataatgcaca aatgattctg caaaaaattc
    25741 ttctataaac ttgcttatat atgcatgtgc aacttgttta gggtcataaa gcttatatct
    25801 ttcctgagca aatacttcca taaattcttc agaatttgat tttttattta aaacattttt
    25861 atctatagca tgtgcagttt catgtaattc taaatttttt gatgaatgta catttgcata
    25921 gctagggtta ctataaccta ttttagctac tatagaatta tttctatata taccaggtac
    25981 atctttccaa gtcttgcctg atcttaccca acatggaggt aattgatatt ttaaatcact
    26041 aaattctggt tcatcagtta gatttgaatt tattaatttt atttttatat ccttttcttt
    26101 aagtgtctgt aaatatttaa tatttatttt tgcaagtctg cttttcatat tttcaacttc
    26161 atctttatca taattcaagt catcaggaag tataagcatt tgttcaatta tatctatgct
    26221 actgtctttt atatcttcag attcttcatc aaatttatat tgaagagtat tattttcata
    26281 gatagttata tttgtattat tttccatata ataagagtga atatcctcta agtctaaaat
    26341 attcatttgt tgtaaaatat gttttgtttc gccactccat actactaaag aaagtgaatt
    26401 aaaattaatt agtggatttt cctttgcttt tatatcattg cttatatcta catcaaatac
    26461 atattcaatt gagtcttcta tgttaagact atctgtattc tttttaatat ataaatatgc
    26521 ttgttttaag tctatataaa tctcatctaa agattcatca taaaattctg ataaattatt
    26581 agtaaagagt tcttcactta aaagttcatt tttatcagtt atactttcaa ttaaaatatt
    26641 aatttctttt atcatatcgt aaatacttat ttttctattt gctttttcac tagatacatt
    26701 gcatggcgca agagcaatgc aataaaaatc tttttctttt ttataaaagg ctttaagtga
    26761 ttttgataaa agtgtaaagt ctacacctat ctgtaagttg accttaagtg agtcattttg
    26821 taataattca gtattatttt ttttagctat atttaaattg ttttccataa tcattgcccc
    26881 ctttttatta gggtatcata atttttttat atgtgtcaat ttgttttttt gaaaattata
    26941 atttttttga aaattttatt ttttataatt tgtgaatttt ttattataga aataaatatg
    27001 ttattattat gtcaaaattt agcattagat tcatgtagag tatgttttat acatatatta
    27061 taattaattc tttgaagatt gcatattcaa aatacacacc atttttttta attctataat
    27121 ataatattta tatatttaat gttaggatgt gatttaaggt gtattttaca tatattataa
    27181 gatgtaaaga tgattctcta tacactggat ataccagtaa tatagttaga agaatgaatg
    27241 aacataagtt aggaattaat tcaaaataca caagagcaaa gggatttgaa aaactagaag
    27301 tttattttgt gaccaacaca aaaagcaatg ctatgaagtt agaatactat ataaaaaaat
    27361 taactagaaa taaaaaattg tctataataa aaaacccaag tatacttata aatttaattg
    27421 ataataaaga agattatatt attggtaagg aaatagaaca attaacataa atatttatag
    27481 taacattata atgggtgcat atagtgtaaa aacttgacaa taaatactat tgtaatataa
    27541 tgaagttata aatatagtaa attaaaatag ctaggggagc caagagctga gaggtatacc
    27601 gacccttaca cctgatctgg ataataccag cgtaggaaag cttagtaaaa attcaagtat
    27661 gtattatttg tgatatatgc tatttctaag ctatacctat tgtaggtata gcttttttaa
    27721 ttaagttatt taaattaaac tttaggaggc aacaatgagt aattataaaa taccaacact
    27781 tacaatagct ggctctgatt catctggagg agctgggatt caggctgatt tgaaaacgtt
    27841 tagtgcaatt ggtacttatg gtatgagtgt aataacagct ataacagcac agaatactaa
    27901 aggagttttt gctgtagagg atttaaataa gaaaataata aaaaaacaaa tagaggcagt
    27961 ctttgaagat atacctccaa gagctgtaaa aataggaatg gtttcaagtc ctgagattat
    28021 acttgaaata gttgagaatc taaagaaata caatccaaaa tatttggttg tggacccagt
    28081 aatgatatct aaaagtggat attatctatt aaagccagag gcaaaagaaa acttaattaa
    28141 gtatttgata ccacttgcat acataattac accaaatata ccagaggcag aagaaataac
    28201 aggaataaaa atacataatg tagatgatat gaagagagta ggagaagaaa tactacaact
    28261 tggacctaaa tttgtactta tgaaaggagg tcatttagat ggagaagcag tggatatttt
    28321 agttggtaaa aatatattta aagtttataa aagtgaaaga attgataaaa aaaatacaca
    28381 tggaacaggg tgcacattat cttctgcaat aacatcttat ctagcattag gatatgaaat
    28441 aacagaagca gtaaatctaa gtaaaatcta tataacagaa gcaataaaaa gaagttttga
    28501 tataggtcat ggagtaggac cagtacatca tttttataaa tttgagtaat aaaaaaatat
    28561 tggaggtaga atatgtataa tttaattaaa gatgtaaaaa aattaaatcc attggtaatt
    28621 cattatacta ataatgtaac tataaatgat tgtgctaatg tgactttagc tgtaggagca
    28681 agtcctctta tgagtttttc atatgaggaa gtagaagaaa tggtaagtgt agcaaattct
    28741 gtagtaataa atataggaac tatgaactca aatatgttgg atttatttct attagctgga
    28801 aaagcagcaa ataagtacaa taaacctgtt gttttggacc cagttggagt atttgcaagc
    28861 aaagcgagag cagaacttac aagtagactt ttaaatgaag tcaaatttag tgttgttaaa
    28921 gggaatgtat cagaaataaa gtttataggt ggctttaatg taaggggtaa aggtgtagat
    28981 tcatttgatg aagaagaaga ttctactgag ataataagaa agattgctga aaagcttgaa
    29041 tgtgtagttg tagcaactgg aaaaatagat ataataacta atggtaaagg tatatataaa
    29101 ataaacaatg gaactgataa attaaaagga attacaggaa ctggatgtat gactgctagt
    29161 ttgattgcaa gttttatggc tgttactgag aatatattag aagcagcaac aatgggtgtt
    29221 cttacaatgt ctttaagtgg ggaattagct aatttaaata atccaccaat aggtacattt
    29281 aaagaaaatc ttatgaatgc tatttatcaa atggatatag atactttaag taaaaattct
    29341 aatattgaat ttttaaatta ataaagagta agggagactt aaaatgattg ataaagaatc
    29401 tttaaaaaaa tgcttaaagt tgtatttggt aactgactct gaaatgctta aaggtagaga
    29461 cttttataaa tgtcttgaag atgcaatatc ttctggaata acaactgtac aacttagaga
    29521 aaaaaatgct tctggaagag agtttttaag aaaagctatg aaacttagag aaataactga
    29581 aagatatggt gttaagttta taataaatga tagagtggat atagcactta tttgtgatgc
    29641 agatggagta catgtaggac agagtgacat tgatgtgaga gaggttagaa agcttatagg
    29701 aaacaataaa attctaggag tatcagcaag aactttagaa gaagctactt gtgcaaaaaa
    29761 tgatggcgca gactatttag gtgtagggtc tatattcaca acatctacta aattagatgc
    29821 aaaatcagct tcgtttgaaa ctgttaaaga aattaaagaa aaagtagaca tgccatttgt
    29881 gctaattgga ggtataaatt tagacaatat agataaactt aaatgtttag aaagtgacgg
    29941 gtatgctata atatcagcta tattaaaagc tgaagatatc tctaaagaag tggaaaagtg
    30001 gacattaaaa atataaaatt ggctctggta gtgaaaaaaa tggaaaaatt tgatataatc
    30061 taagagtaaa atttaagcaa ttataaatga taaacttttc tacatatact ttttaatcaa
    30121 gatatatgta gatttggtta aaaaggagct ataaatgatt aagatattgt tagtgtgtgt
    30181 tggaggcatg tcttctaccc tattggtaaa taaaatggag aaggatgcaa aaaaaagaaa
    30241 tatagactgt aaaatatggg cagttggaga aggagatata aaatcggagc ttgataactt
    30301 tgatatttta cttcttggac cacaacttag atttatgctt gatgacgtaa agtctatagt
    30361 tggagataga gttccagtat ctataataga tatggtaaac tatggtactt gtaatggtca
    30421 tgctgtgtta aattcggttt tagaaatatt aaaataaata ataaaagtgg attctatata
    30481 atagagtcca tttttattat ttgttattaa taggcttaaa acttagctta ccatttcttt
    30541 ttttaatctc cacatagcaa gtgaaaaact tgaacagcac aatattaaaa atagtaggat
    30601 aacaggcagt atatctccaa taagaatagt ctcaccaaat attaagctac gtaaagcgtt
    30661 aataacatgg gtaaatggat tgagcatcac tgctactttt aatcctccag ataaactttc
    30721 tatagggaaa agagctgaac ttaaaaagaa aataggaagt acaattgcag tcataattgt
    30781 ttcataaact atttcatttg gcaataataa acttatagaa taagctagac ttgataaaaa
    30841 gaaggctgtc ataaatatca gtactatcat aagtaggatt ccaccaatgc cagattctat
    30901 ccttactgag aagaatatac ttactataca caaaatagtt acttctatga aggaaactaa
    30961 gatagcttct aataattgac caaggacgat tgaataccta ctgataggtg caattaagac
    31021 tctatagaaa cttccactgt tcttcattat aaagtttata ataccaccac tactacaaga
    31081 agaaaacaca accagtacaa tgatacctgg tagtataaaa gcagtataat tattgatatt
    31141 tatattattc atagtttggt ttgcaataga actataaagc acaagccaaa gaaaaggttg
    31201 taatatagta actacaaatg agattggatt tttaaaacgc cacttcatac tacgccataa
    31261 gatatttaac attcccattc ctcctttaag ctttttttag aacttgtaag tgctaaaaat
    31321 acatcttcga gacttggctc tacaatttct attgcatcaa attctatatc atgttctaaa
    31381 agccatttat ttattaaagt aaaagcagtt ctactatcgt ttacacttat aaaaattgaa
    31441 ttttctcgta cgctcataaa ctttactaaa ccagtacttt taattgaatc tttatatttt
    31501 tttatatctt cagtattatg gaaagttata cgtagtatat tttgtctaat gtaactacgt
    31561 aaacttgatg gtgttccttg tgctaaatcc tttccattct tcataataca aatattatca
    31621 ctcaattgtt cagcttcttc aagataatga gtagttagaa atatagttgt gccgtactca
    31681 tctctaattt ttaataacat atcccacata gattttcttg aatcgacatc cataccaaca
    31741 gttggttcat ctaaaaatag gattttagga gatgatacca tattcatagc tatgtccaat
    31801 ctacgtttta caccccctga atatgatgaa gtaggatatt ttaagtaact agataagtca
    31861 aacttatcaa ttaaagagtc tattctttgt tttgcaatct gtggttctac tttatacatt
    31921 ttactttgaa ataccatatt ttccataagg gataaatgtt catcaataga gatttgctgt
    31981 gctacacaag caatttgagt acgtatccaa gaaggattat ctactaaatc ttttccaaac
    32041 atttttacat ttccagatgt aggtttgtaa aaggtagtca aaatatttat aagagaagat
    32101 ttaccagcac cattaggacc aagtaaagaa aaaatttctc cagcatttac attaaagctt
    32161 agaccattta aagctttcac accattttta tattctttta ctaaattgtc tatttcaatc
    32221 gctaacatat attattcctc ctctttaagt ggaaatatta tctctgtaat atagttatct
    32281 gggtttcctt ttatcaacat acctggtcct ttaataaata cttctctcca aggagtttgt
    32341 atttttatat tattttcatg aatatattta aatatcattt catacgtttt tggcaaatta
    32401 ctataagagc ctgtgtgagt aaaaaataaa gcttttgttt taggaaattc ttttgtagtt
    32461 atacccatac gatttggagt ttcagcagtt ggaacacata attccatttc tgcaacacca
    32521 gttttttggt caacatcata gtagcaaaag aatggtgcac cgttagactt tccttttata
    32581 gctttaaata catttggaaa atactttgtt gcctcagtca taggtccatt ataacgcatg
    32641 gtagccactg tgactgattc gatatttttg atttcaattt tgcaattcat ttgtttacct
    32701 cctaatgtgt gttcatattc ttactagatt tttaagcatt aatacatata agattttgat
    32761 tagttgaaaa catataatct tatctcgttt gcttgttttc aagtctaaca taacataaaa
    32821 tatatatcct ctcatttgat gctccatttt gtaaagctat tatttaagct ttactttaga
    32881 ttttttagca aattgaaata aagcaattta taaaataagt atttacaaat taagtgtatt
    32941 aatcgtataa tacacttaat aggatactag ataaagtctg aaataaaggc gtattttttt
    33001 aaagtaagtg tactatcgta ttaatacact agaataggag gaatcatgaa ttttgaatta
    33061 gataatacaa ctcccattta tttgcaaata gtaaagtata taaagagaca aatagtaaca
    33121 ggagagttga aaccaggaga gacaatacca tcaagaagag aaatggcact taatctaaaa
    33181 gtaaacttaa atacagttca aagagcatat aaggagatgg gtgatatgaa tataataaat
    33241 acttttaaaa attatcaaag cagtgtaaca gttgatgaaa atatattaaa aaatttaaaa
    33301 ttagagctta taaatgaatc actatctgtt tttatagaag atatgaaagc aataaatgtt
    33361 agcaaagaag aagttttaaa aataatagaa gataagtatt agttttttaa caaatagaaa
    33421 acttagttta gaaagtttat ttataagcat attggatgtg gaggatagta aatatgatag
    33481 aaataaagaa tgtcagcaaa acatataaaa ggatgcaagg gcttaaaata agaaagatag
    33541 aagctttaaa aaatgtaagt tttaatatag aaaagggaaa aataactgca ttacttggta
    33601 taaatggtgt tggtaaatca actatgttaa aagcaatagc aggacttata aacattgata
    33661 gtggagaaat tcgtatagat ggagaaaaaa taaatgaaaa ggtatacaat aaattggctt
    33721 ttgttccaga tgtacaatct cactttagca atacaacaat aaaagaaaca tttgaattta
    33781 tggaaatttt ttattcaaag tggaataaag aaaaatcaaa agaaatgatg gacatattta
    33841 aattggatga agatgaaata attgataact tgtcaaaagg aaacattgca agagtaaaat
    33901 taatacttgg attttgccaa gacccagagt acatacttct tgatgaacca tttacaggaa
    33961 ttgatttgtt taagagggaa gagtttatag gagtaatagc gcaatatatg gaggaaaatc
    34021 aagccattat cataactact catgagatag tagagataga aagtttagtt gatgaggttg
    34081 taattcttga tgaagggcag attataactt catttaatgc agaagaatta agagaaagag
    34141 aaggtaaatc tatattagat aagatgaggg aggtatataa gaatgaataa acttctatat
    34201 ctaataaatg tagatttgag aagaagcaat aagttttata tagcttatat atcatttttt
    34261 tcattaatta cacttagttt aaatttattc gaaattaata aatttaaaaa ttctaaattt
    34321 attatgaact tagcaataga agattttgga ggtatatttt atggactagg gatacttagt
    34381 aatcttggtt ttatccaaaa tatattgttc tttggaatgt tgggactttt tatatactat
    34441 atttttatgt ggaaaagaga gttcttttca aataatcaaa gtatttatac acttatgatg
    34501 ttacctcaaa ataagtttaa gatttgtata tctaaatcaa tagttttgat aacaatgata
    34561 tatggttttt tgataagtca gacagcaaca ttatttatat gtaaatatat atttaatttc
    34621 atttttagaa atattcctat aatcaatatg aactttgcca aagatttata ttatttaaac
    34681 ttgaagagta taccaattga ttttatctct tttatggcta tttatgtatt tttactactt
    34741 atagctattt ctattgtaaa ttgctgtata ttatttatct tttcctatat aaataaattt
    34801 tatgtaattt taatcacttt gctgattata gcaggcttat atttttcaac aatatatata
    34861 aatataatca atttatttag aaaatattat aatcttatag gatttcaatt taacaattta
    34921 gtagcagggt tattaagttt tttaattata atgtttattc taaatggaat atcttatttg
    34981 ctaattaaaa agaaagtatc tctttaagga ggggtggtat gaaattaaaa tctaaaatat
    35041 ctatacttat tatatttttg actttagcaa tatattttag tataggttta ttaaataatg
    35101 aagataattt aaagataaaa actataaaag gtgactctaa tgaattagac catataaaca
    35161 taaattttgg atatgctttg aatggatata taaattcata ttatacaata aatggaagta
    35221 acaaaactaa aaagactgaa ttaataaaac tacaaaagga tagtaagaac acaaataatt
    35281 taattgatgt atctagtggt tcagatttaa ttgctcatgt tggatttgaa atgaatgagg
    35341 agtcaaaaga tataaacttt gtgatagatg agaaaaaaga agatttagaa aaaggtgtat
    35401 ttccatcaga aggtagtgat aaagaaattg aatatgaaaa attagatatg aataatttta
    35461 caaggacaag atataaagta gataaaagta cagatgatta ttatgcaaat atactagcat
    35521 caaaaagata taaaaaatac ttatatgtat ctgtacttta tgctacaagt gattcatcag
    35581 aaaataacaa agaatattca agtactatag atataataaa aattaatcca gaaaataaaa
    35641 aaatcaagac tataaaaagt ataaatttaa gtaattattt ttccgagaaa taccaagaca
    35701 tagagaacat aggtgtaagt tatgctagca aatatgagga taagatgtat attttagtaa
    35761 atgtactaaa tacaaacata ggtgataata gtaattctaa atttcattta cttgagtata
    35821 atcttgaaga aaataaggaa agcataatgg aattaaaaac tgatagcaaa atggctttag
    35881 caaaagcaaa gttagaagct aataaattat atatggtaat gtcgaatgat tatgataata
    35941 gttcaattac agagttaaat ttacttacct ataatatatt agataaaagc ttagatgaag
    36001 ataaatatgt tttagataat agagaaaaaa gaggaattag aagtattgaa attgattcaa
    36061 ataaaatcgt tgcatataca tcaaggctaa actctatatt atcttgggat aataaattta
    36121 tgctatatat aataaataaa aattctaagg aaattgtata tgaaggagaa ttacaattta
    36181 acagttcaaa tagtatgtta gaatttgaaa taaagtaggt gagttgatga aagatttaat
    36241 aagattattc gaatttgaat taaaaagaaa tataaagaat tatatattta taataatttg
    36301 ttgttgttct tttgtaattt taaacatagt aaaaaattta aatgactata attacataat
    36361 agaaaatgca gttaaaagtg aacagttaac aaaaatcgga aatacgatag aaacggtaaa
    36421 tgttacaggt ttctcaagct ttagaaatat aatgggttct acagaatcgt ggtttatatt
    36481 tggtataatt gcatgtattt gctatgcttt ttttatttgg tatagagact ttaatggtag
    36541 gagtaaatct atatatacac ttataatgct acctaaaaat agaatcaata tttatatatc
    36601 taaattatta aatatattat ttctagtgta tagctataca gtagtactta caatatcttt
    36661 gtttattgca agtaagttgt tgcctagaca tatgcttggt aatgtcacaa actatggttt
    36721 tgtacaagaa acaatatatg agttaaaaat gttacttcca tatagctttg aaatactatt
    36781 tgtagagtat atatttttat tgataggatt tgtatctgta gtatttacat caattcttat
    36841 aaataaatct atttataaag taagcacttt aataagtttt ctatttttag ttttggaaat
    36901 tttagtgttt ataatttcta tagaatttat tataccttat gaatacagtg atgtatttac
    36961 tgttctatat tcaagtataa acatatttgt atgtagtctt atatctaata aacttttgaa
    37021 gcaaaagatt gacttttagg agggatatta tgaaagcaaa aaatatagga aaatatacaa
    37081 tatgttttat aatagtatgt tttttaatga ttactatgac cttaatattg agttatttta
    37141 ataaggataa actatctgta aaaactttaa aaggcaatgt aaaagatata gggagagtaa
    37201 gtgtagtata tacacctaat ataagcaaat tttcttatga ggaggtaatt ataagtaaaa
    37261 atggagtaag aaataataaa aaccccaaat caagaattag aaatgattat ttaaatattg
    37321 acaaaaaata cagtgatttt gttaatgata aaggaaaaga acttatatat aaagatgatt
    37381 tgaatatagg agaagcgtat acctctgtta agtctgattt agtacaagga atgaatgctt
    37441 taaatgattg tataaaggtt tcaaataaaa atttaaaaag tggggtagaa gaaaattttg
    37501 atattaattt aaataagtct gattttaaag aaaacagttg gaaaaccttg tttaataaaa
    37561 tatataaagg caatttatat atggtaatta gtgaaactaa tatttcaggt tctaaggtca
    37621 ataatatttg tatattaaag atagatataa aaaataagtc ttataaatta ttaaatagat
    37681 ttaatttagg attacagaat ggatatcaac aaaatatcaa tgaaattaaa ttttctattg
    37741 gaagtaaatt atacttagaa atagtaaata ataaaagtaa aaatggagat attgaaggac
    37801 atgatagtta ctctaacctt attatatatg atatagaaga taacactttt aatataagta
    37861 ataaatttat ggataatcta aacaaagaaa agaatgcaaa tccaaaggag attggcttat
    37921 taagtaaaac taagctagat tatatgataa aaaataataa attgaatctt atagttacgg
    37981 atatgagtaa ttccataatc aatcttgttt atgatattaa ttctaatgag attaaattag
    38041 aagattatga gatacttgat ttaaaagtag attataatga agcatcatct ggagttagga
    38101 aagttaagtt actggatgat aagatttatt ctatttcagg tatgaagaac agtatatcag
    38161 gagaattaaa aggatttaaa gggattgttg atgtagtcgg aactaagcca gtaaaatttc
    38221 aagtatttga tatgaaactt aaaaagaata tttatgaagc tgagctaata aatggagata
    38281 taagtttaca agaaaagtta ttttttgttg caaattaagt attagaatta attttagaac
    38341 tttaaacttt tatatgtatc acagataagt ataagtaagg gagtgtctca aaataaactt
    38401 tttagttcat gagacacttt ttttatatga aatcaaatat attttcattg tttagattta
    38461 ttttgataat gaaaaaaaga ttatctttat tttaagaaag acatttattt ataattatat
    38521 acaaaaatat tttttaatgt ttttgtgtat aattataaca atttaattta tattaatgca
    38581 cttaaatttg aaatagaatg cttatagtgg taaaattaat tattagaata ttagaaataa
    38641 taaataaata atacattgta cttaaaaggg ggagaaattt aagatgaaca aaaaatatat
    38701 taaagcaggg aatttaattg atggaacagg aaatagtatt atgcaaaata agggtataat
    38761 tattgatgga gatactatag ttgaaattga agatataaga gatgatttaa atggatatga
    38821 tgtgtacgat tacttagata aaactgttat gccaggcatt atgaattgtc atgttcactt
    38881 gacaatggag ccagttggaa atccaaagat atattatgat aatgtatcag atgtagagct
    38941 tgtagtaaat acgataaaac aagttgatgc ttatttggat tcaggagtaa catatattag
    39001 aagcttaggt tgtcccaaat atgtggatgt acaacttaaa aatcttatag acaaagggtc
    39061 tatagatggt cctggtatag ttacttcagg tccagttata tgtatgacag gagggcatgg
    39121 atattatttt ggtattgaaa gtgatggagt ggatgaatgt agaaaatcag ccagaacagt
    39181 tttaaaaaat ggagtagatt gtgtaaagat aatggcaact ggaggtgtaa caactgatgg
    39241 agttgagcca gggtcaccac aattaacttt ggaggaaatg aaggctgctg tagatgaagc
    39301 tataaaagca ggtaaaataa ctgccacaca tgcacaagga agaactggta taaaaaatgc
    39361 agttcttgca ggaattactt ctatagaaca tggtgtttat ttagatgatg aaataattga
    39421 tttgatgctt gaaagaggaa cttacttagt tccaactgtg gctgcaccat attttattct
    39481 aaatgcagga aaagaaagtg gaataactga agctactctt agaaagtgtg agattgttgc
    39541 cattccacat aaagaatcat tcctaaaagc atataaaaaa ggaattaaaa ttgcagttgg
    39601 ttctgatgca ggaacttctt ataatgaaca tggaaaaaca tattatgaaa tgaagctaat
    39661 gtcagattat ggtatggata atatggatat aatagttgct gctacaaaaa cagcatctga
    39721 acttttaaga attgataaaa attatggaac tctagaaaaa ggaaagaaag cagatgttat
    39781 agttgtaaat ggaaatccag tagagaatat agacgtactt gcagatgtgt tagcagtatt
    39841 taaacttggt aaaaaagtta gataaatagt ataaaaataa aaaagagtgt ctaaagaaat
    39901 ttttaaataa ttatagacac tcttttttat tagtaataat taaatctttt atactttaga
    39961 aaatagattc tattttatat ttagatataa ctctttaaaa tcattcttat gcaatataat
    40021 ctatagaatc tacacataca aagtatattc ttgaatcatt agaatttcca agtactataa
    40081 catcattttt cttacctaga acttcaacac cagttagaac caaactacca gctactacag
    40141 taacttgatt atttatattt gacatataaa gattatctag gataccttta ttacaacagc
    40201 aatcatcaca atcgcagtta caattacatt ctttgtaatg ttttttatca agatagtaac
    40261 ctaaagatgc tatgaaatct tcataatctg caggtgtaat atcaaatgca acagctttaa
    40321 gattacacaa tgatgctaaa tctacgtttg ctaatggagt agatgcagca agtataaaat
    40381 caattatagc agcaagtatt gcatctaata tagccccaag gtctatcgta ggaggaataa
    40441 ctgcatcaat aagtgcaatt aattcagcta ctcctggtat agttcctata gtattaatta
    40501 atccctcaag agttaaaggg actggaatag gataagatac tctaccagct atatctatta
    40561 aatcacagtt acaagcagaa aatctttcaa aagtaccatc aagtccagat aaattatctt
    40621 ttggaggagc tgaaagatct atacctacca aattagcacc tactataaag aaatctgaga
    40681 taaaagcaaa ttgattaaag tttacaaaag gtcttagagc atcatatctt agaagttcta
    40741 aggcctctct catacttttt ttacaacaac attgttttat tttaggttct ttatatttat
    40801 ttggataatt atagtatcta tcagctactt gataattttc atagtatctt tcatttgtat
    40861 ttggataatc ttcctcatat tcttgtgtaa atctaaagtc ctctctacat ttattatttt
    40921 ccactaaata cactcctttt atattttaat ttaaatgtat agataattaa gcttatctat
    40981 atagtaatat tatatttata atgtaaaaaa atgttacaaa agatattata aacgaataaa
    41041 aaataataat ttgtcagata aacttgtaaa tttttatgaa taatccattc taaaacaaaa
    41101 aactgtgtat aaaaaaggtt tatatgttat atttatataa atataacata tgaacgagaa
    41161 aggagtggtt tgataaatga ttgaggtaga taaattgtgt aagtctttta caagggttgt
    41221 aaaagatgat aagaataaaa gttcaataag aaagctaaag aaagtaaaaa ctaaaaaaga
    41281 agaatttttt gctgtaaata atgtttcatt taaagttatg gaaggtgaaa ttgttggaat
    41341 attaggtcca aatggtgcag gaaagactac attacttaga atgttaggtg gcatattaac
    41401 accaacatca ggaagtataa atatttctgg atatgattat tcaatagata gaaattcagc
    41461 aaaaaaagaa ataggttatc tctctggaaa tacaaaacta tatggaaggc tttctccgag
    41521 agaacttcta acaacttttg caagtctata tgagatgtca aaagaagata tagaagaatc
    41581 aattgaaaat attgtaaaaa taatggatat gagtgaattt atagataata gaattgaaaa
    41641 tctatcaact ggtcagactc aaagaacatc tatagcccgt tgtttaatac attctccaaa
    41701 agtatatatt tttgatgagc caacactagg tctggatgtt ttaagtagtg catctattat
    41761 agattttatg aagaatgaaa aactaagagg aaaaactgtt ctttactcaa ctcattatat
    41821 ggaagaagca gaaactttat gtgacaagat ttttatgatt cacaatggga aaataattgc
    41881 atcaggaaca ccacagagtt taaaagagga atctggagta aataatttga gagatgtatt
    41941 cataaaactt gccagaaagg aggacattta aatgagaagt aagattgtaa aatacatatt
    42001 taaaaaagaa atgttagaca tattgagaga taaaaagact ttatttatga tgattgttgt
    42061 gccacttctt ttatatcctt tacttatgtt attaatgatt caagtaatga atatgtctgc
    42121 aagtagtatg aatagtaagg acattaatat agcttttaat aataaaccta ataaggtgtt
    42181 agtatccatg attgaagatg ataatttaga aaaagatact aaaaatactt caaatgataa
    42241 aggcaaaata agagtgttag atgtagataa ctataaaaag tcattagaag aaaataaaat
    42301 tgatgcttat ataaagatgg aagaaaaaaa atcatctata aattatcaaa tatatataaa
    42361 ttcttctcaa gaaaattctt ctaatgcact aaaaagaatt gaaactgttt tggataaata
    42421 taaaagattt accatagaaa aaactttatc tgaaaaagga ttagatgtac aagctacttt
    42481 agaaccaata acatatagca ctgtagatgt tgcaaagacg gaagaagtgg caggatactt
    42541 tttaggtcag atacttccat ttatattaat aataggagtt ttactaggtt caatatatcc
    42601 agcaatagat gttatggctg gagaaaagga aagaggtaca ttagaaacat tatttacact
    42661 cccaatatca aacttagaac tggttatggg aaaatatatg gcagtttctc ttagtgcaat
    42721 agtaacagca attttaaaca ttttatctat gggactgact atggcattta ttttagttag
    42781 tggaggtatt agtagccaat ttggctttgg aaactttaat tatggagact tagtcttacc
    42841 aattataact actttaattt gcatatgtct tttttcaatg gtagtttcag caataagtat
    42901 gtgtgtatgt tcattagcaa aaagttttaa agatgctcaa aactacataa cacctcttat
    42961 gttaattatt atgataccat catatgtttc tatgatacca aatatagtat tggatagagt
    43021 gacagctgta atacctgtag taaatatatc tttacttata aaaagtgtat tatcttttaa
    43081 aagcgattta aatttaatag ccttagtttt agcatcaaat gtagcctttg ttatattggc
    43141 aatcatacta ctgtctaaga tgttcaattc tgaggaaata ctttttggta atagtaagag
    43201 tttttctttc ttagagaaaa gaagtaatat aaaaaaaggg tctataccaa ctgtaagtga
    43261 tggtgttatt ttatatgcag taggattagt tctattgatt tatgttggtt ctttagttca
    43321 aattaaattt ggtatggcag gtattataat gacacaattc atgataattt ctttaccatt
    43381 attatttgca tgctatatta aagctgattt taaagaggta tttagcttga cagttcctaa
    43441 attaaagcat gtgtttggga gcattgttct ttggattggt ggatacatac ttattatgat
    43501 tataacacag ctaattttat tcttatttcc tcaaaatatg gaagttgctg aaagtttaaa
    43561 taaggctctt tttatcaaag atagtgtttt actgaattta ctagcaatag ctttattacc
    43621 agctatatgt gaagaactgt tttttagggg atttatattt tcttcattta gtaaatcaaa
    43681 agataaaaat aagtctatca aactcgctat aatatgtagt ggggtactat ttggtattat
    43741 gcatatggat tttattagaa taattccaac atcaatatta ggtattatat ttgcatacag
    43801 tgtgtataaa tctggttcaa tatttgtatc aatgttattg cattttttaa ataatagtgt
    43861 agcagttgtg ttaaatcatt atacaactgg aaatataacc aacttatata aatttgttga
    43921 agttgatttt tcgaacttaa atgtacttaa atttgcctta attcttctaa taagtcttat
    43981 attaattatg ttaggattaa gagttttaga tagaaagtct ttaagaaatt aagtataaag
    44041 ttattatata gattaaaata ttaataagca ctaaagtata tggatatatc ttgtatataa
    44101 aaatacttta gtgctatttt atatatttta attatatgaa ttatttatcg tttcatttat
    44161 gtaatgaaaa tgttatataa atgaaacaat aaataatttt tattttagaa atttatattt
    44221 aaataatcaa gttgaaagtg agatactaaa aaaattaatt taggaattat ttgatttatt
    44281 catttataaa aattaattac tgatttgtcg aaaccattgt aataagaaaa aaatgaaaaa
    44341 ataaagtgtg aatatgttgc aaatactgta ttttaa
//
LOCUS       DAEPOZ010000007        39771 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.007, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000007 DAEPOZ010000000
VERSION     DAEPOZ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39771
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.007"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            235..798
                     /gene="raiA"
                     /locus_tag="KR440_000318"
     CDS             235..798
                     /gene="raiA"
                     /locus_tag="KR440_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBH0697387.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            1011..1247
                     /locus_tag="KR440_000319"
     CDS             1011..1247
                     /locus_tag="KR440_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HBH0697388.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            1260..2603
                     /gene="yqfD"
                     /locus_tag="KR440_000320"
     CDS             1260..2603
                     /gene="yqfD"
                     /locus_tag="KR440_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HBH0697389.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKEFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            2542..3657
                     /locus_tag="KR440_000321"
     CDS             2542..3657
                     /locus_tag="KR440_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBH0697390.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKVKMQEKTFKRK"
     gene            3714..4175
                     /gene="ybeY"
                     /locus_tag="KR440_000322"
     CDS             3714..4175
                     /gene="ybeY"
                     /locus_tag="KR440_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBH0697391.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            4181..4900
                     /locus_tag="KR440_000323"
     CDS             4181..4900
                     /locus_tag="KR440_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBH0697392.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            5019..5420
                     /locus_tag="KR440_000324"
     CDS             5019..5420
                     /locus_tag="KR440_000324"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBH0697393.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            5484..6377
                     /gene="era"
                     /locus_tag="KR440_000325"
     CDS             5484..6377
                     /gene="era"
                     /locus_tag="KR440_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBH0697394.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            6389..7768
                     /gene="mgtE"
                     /locus_tag="KR440_000326"
     CDS             6389..7768
                     /gene="mgtE"
                     /locus_tag="KR440_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBH0697395.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            7841..7996
                     /locus_tag="KR440_000327"
     CDS             7841..7996
                     /locus_tag="KR440_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HBH0697396.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            7962..8735
                     /gene="recO"
                     /locus_tag="KR440_000328"
     CDS             7962..8735
                     /gene="recO"
                     /locus_tag="KR440_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBH0697397.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            8728..9327
                     /locus_tag="KR440_000329"
     CDS             8728..9327
                     /locus_tag="KR440_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HBH0697398.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            9487..10365
                     /gene="glyQ"
                     /locus_tag="KR440_000330"
     CDS             9487..10365
                     /gene="glyQ"
                     /locus_tag="KR440_000330"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBH0697399.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            10365..12431
                     /locus_tag="KR440_000331"
     CDS             10365..12431
                     /locus_tag="KR440_000331"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBH0697400.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQSDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFNKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIRLYNEFKQVKVKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLEICDL
                     SKIVYK"
     gene            complement(12597..14144)
                     /locus_tag="KR440_000332"
     CDS             complement(12597..14144)
                     /locus_tag="KR440_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HBH0697401.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     VENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
     gene            14424..15191
                     /locus_tag="KR440_000333"
     CDS             14424..15191
                     /locus_tag="KR440_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBH0697402.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            15474..15671
                     /locus_tag="KR440_000334"
     CDS             15474..15671
                     /locus_tag="KR440_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0697403.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            15700..16776
                     /gene="vorB"
                     /locus_tag="KR440_000335"
     CDS             15700..16776
                     /gene="vorB"
                     /locus_tag="KR440_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBH0697404.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            16779..17498
                     /locus_tag="KR440_000336"
     CDS             16779..17498
                     /locus_tag="KR440_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBH0697405.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            17511..18050
                     /locus_tag="KR440_000337"
     CDS             17511..18050
                     /locus_tag="KR440_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBH0697406.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            18207..19265
                     /gene="buk"
                     /locus_tag="KR440_000338"
     CDS             18207..19265
                     /gene="buk"
                     /locus_tag="KR440_000338"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBH0697407.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            19283..20176
                     /locus_tag="KR440_000339"
     CDS             19283..20176
                     /locus_tag="KR440_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBH0697408.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            20206..21414
                     /locus_tag="KR440_000340"
     CDS             20206..21414
                     /locus_tag="KR440_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBH0697409.1"
                     /translation="MKYAKRMDMVKASAIRASQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDIVVTENPSYLGAINAFRPYECDFLGVDTDDEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            21438..22700
                     /locus_tag="KR440_000341"
     CDS             21438..22700
                     /locus_tag="KR440_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0697410.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFSTPVLGMRWVMISLGILSIVMCPVLWFVIKDVDIREDKEEEASEKMSAGDFM
                     TVLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFG
                     APFGGYCADKVKSPTKVLLPIYVFGIAVIILFLVLPASTPMTIFIALTFVVGILGYMG
                     KGIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIF
                     QIAILIIGILSCLYILKVKKKRESKLEA"
     gene            23276..24739
                     /locus_tag="KR440_000342"
     CDS             23276..24739
                     /locus_tag="KR440_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBH0697411.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSMKLRFFQIKDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSILFSVVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVTSSIALGIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIIANINLVPGAIMTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIIITGMYNVTDVYTTLPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKHIIN
                     VCRFMLIICAAMCCFIEPLTSWALGDICIGVLTYINLVGIFTLKKPAIKAYYDYQRQK
                     KMGVKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            24795..25931
                     /locus_tag="KR440_000343"
     CDS             24795..25931
                     /locus_tag="KR440_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH0697412.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            26167..27282
                     /locus_tag="KR440_000344"
     CDS             26167..27282
                     /locus_tag="KR440_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HBH0697413.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALTPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTVMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLEPQSMKNSIHRYKL
                     IKEYINKAKKIGEKELMNLLSLKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            27434..28303
                     /locus_tag="KR440_000345"
     CDS             27434..28303
                     /locus_tag="KR440_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH0697414.1"
                     /translation="MDYLKYFEKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSKRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
     gene            28679..29224
                     /locus_tag="KR440_000346"
     CDS             28679..29224
                     /locus_tag="KR440_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBH0697415.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
     gene            29237..30250
                     /locus_tag="KR440_000347"
     CDS             29237..30250
                     /locus_tag="KR440_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBH0697416.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            30472..32445
                     /locus_tag="KR440_000348"
     CDS             30472..32445
                     /locus_tag="KR440_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBH0697417.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKENNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDDKFRKNIVNREEKSPTVFD
                     KGLLFPHSVNEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            32514..32873
                     /locus_tag="KR440_000349"
     CDS             32514..32873
                     /locus_tag="KR440_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697418.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAIISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            32908..33264
                     /locus_tag="KR440_000350"
     CDS             32908..33264
                     /locus_tag="KR440_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBH0697419.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            33301..34593
                     /locus_tag="KR440_000351"
     CDS             33301..34593
                     /locus_tag="KR440_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBH0697420.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDINLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
     gene            35029..35667
                     /locus_tag="KR440_000352"
     CDS             35029..35667
                     /locus_tag="KR440_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0697421.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            35674..36516
                     /locus_tag="KR440_000353"
     CDS             35674..36516
                     /locus_tag="KR440_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBH0697422.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            36577..39204
                     /locus_tag="KR440_000354"
     CDS             36577..39204
                     /locus_tag="KR440_000354"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBH0697423.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            39418..39663
                     /locus_tag="KR440_000355"
     CDS             39418..39663
                     /locus_tag="KR440_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBH0697424.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
BASE COUNT        15653 a         4228 c         7472 g        12418 t
ORIGIN      
        1 gcaggatttt tgaactattt tatatggttt attaataaat agaatgtaaa gattttgtaa
       61 attaccctgt acttttaata atatcataac aaacgaaaaa taaatagact attaaaaata
      121 atcattttca tatacaataa tattaattta aatacaataa atttataaaa attgtattta
      181 aagattaaca tattaaagta ttagaattgg aaaggcttga agggagaggt ttttatgcaa
      241 ataatagtat ctgggagaca aatgaaatta actgacggaa taaaaggata cgtagatgga
      301 aaattaagta ggttagaaaa ataccttgat cctgaatcag aagtgaaagt aacagtaagt
      361 gcgaaaaagg atagacaaaa agtggaggtt acaataattc ctattaacgg tcaaataata
      421 agagcagaag atgtagaaga tgacttatat gcagcaattg atattgtttg tgacaaatta
      481 agtagacaag ttgtcaaata taaaactaaa gttaaagaca aagttcaaaa taataagagc
      541 ataagatttg aaaatcttga ctttatagat aatagcagtg aatttgatga ttatgattat
      601 gatgatgaag aagatgaaaa tatagtaata gaaagaagaa agaaatttaa tgtaaaacct
      661 atgagttctg aggaggcgat acttcaaatg gaactagtag gacacaattt ctatatgttt
      721 agaaaccaag ataattttga aataaacata gtgtataagc gtaaagctgg tggatatggt
      781 ttaatagaac aagattaata aaaattgtat acgtttagat tttatataag aaaaaataaa
      841 aaggtaatca tgttgatttt ttatcaatat gattaccttt ttattgttta aataatatat
      901 tagatattag gtatttatta tataaaaatt ttaaaactaa tctgttacaa catcaataaa
      961 gtgcataaat acctatttca agcataaaat taattaaggg ggtaatgctt atgttagaaa
     1021 tatccactga tttacaagta aatcaaccag ttattactgt tacaagtaat acgtttatta
     1081 gtatagaaaa ttatctatca atattagaat atgaagttga tttaataaga ataaagacta
     1141 aagttaaaac aattaaaata tctggggata aactttcact taagtatatt actgatagtg
     1201 agataggtat aaaagggatt atctacaatg ttgagtatgt tgattaggag gagatacact
     1261 tgataagttt tataagggga tactatgtta tagttgtgga aggagtagga ctcgaacaat
     1321 ttttaaatca tcttataaga aatgggataa gtgtttacaa tgtaactaga ataaaaaata
     1381 ctaaaatgga gtttcatata gatagacaag atataaaaga atttaagaat gtttatagag
     1441 gaagtaagtt tgatataaag gtaaagcaaa aaactggggt cccttttatt atcaaaagag
     1501 tttataagca caaaggtatg tggatttgtg ctcttgtttc tttattttta ttgatgtcta
     1561 catcgcagtt tgtgacagat gtatatatac aatctcctga aggtataaaa aaagaagctc
     1621 ttagaaatga gctttataag gttggagtaa gaccaggagt ctataagaag agtattgata
     1681 gaaaagaagt tagagaccat atgatgtcaa aatttaatga tgtggcatat ttatctatca
     1741 atgtaaaagg aacaaatata tttgttactg ttacaaaaaa ggcagaatcc ctaaaatcta
     1801 cagaccaatc aaattattgt aatgtaattg ctttaaaaaa tggtataata gaaaaggtaa
     1861 tccctagaag tggtaaatca gttattaaat ctggagacat agtccaaaag ggagatgtct
     1921 tattaaatgg agctaatacc aagtcaatac cagaagtatg ggcaagtact ttttatgagt
     1981 ccacaaaaaa ggcaagttat gtagatactg taaataaaaa aactggagaa aagaaaaata
     2041 tatatacact aagtttttat gataaagagt ttacaatgag aaaaaatata aaatataaag
     2101 attatgttgt tgaaaataaa gaaaaaaaac tatcaatagg aaactatact tttccaataa
     2161 agataaaaac gagtactttt tatgaaacaa aaaaggttga agtaaaaagg aataaagaag
     2221 agttaaaaaa agaattatct gaaaaagctt taaaagagtt agaatacata atcccagctt
     2281 cggctagaat aatagatgta aaacataact ttaaggttaa taaaaatatg ttagaatatt
     2341 taataacagt tcaaactagt gaaaatattg ctaaaattta tcctcttagt aagtcagaag
     2401 cagaaaggtt tataaaggaa gaaagtaaac cagatgaagg tgaagaggaa gttccttcca
     2461 atccagaaaa aagaccatta aatgatataa gaaatgagtt tgatgaggat aataaggata
     2521 ataaagacca aaacaattct gatgaaaata atagtaatca aaatagtaat aatagtcaaa
     2581 ataataatag caataacaac taataaaatt aaacttaaaa tcttaggagg actaattcaa
     2641 ttgataatac aaaagaaatt tacaatagca gatggtaact ttgaaagaga gttatttggc
     2701 aactttgatg aaaatgtaaa attaatagag aagactttaa atatagatgt aatacttaga
     2761 gaaggcaata taattttaat tggagaagaa aaaaatgtag attctgcatt aaaacttatg
     2821 aatgaattac accaaactgt atcaaatggt aagcatttag ataagcagag tatttcttat
     2881 tcattatctc ttttgcttga aggaagtgaa caaaaaataa aagaacttga ggggacaatc
     2941 gtcataactc aaaggggaaa agctgttcaa cctaaaactt taggtcaaaa agaatatata
     3001 aaacttatag aaaataatga tataactttt ggtgttggac ctgctggaac aggaaagact
     3061 tatcttgcgg ttgctatggc agtaaaagct tttaaaagag atgaagttag tagaattata
     3121 cttacaagac cagctgtgga agcaggagag agtctaggtt ttttaccagg agatttaaag
     3181 gacaaagttg acccatattt aagaccacta tatgatgcct tatttgaaat gttgggtgct
     3241 gataaattta ataagtattt agaaagaggt actattgagg ttgcaccact tgcatttatg
     3301 agaggtagaa cacttgataa ttcatttata atattggatg aagctcaaaa tactacttct
     3361 gaacaaatga aaatgttttt aactagactt ggatttggct ctaaagctgt agttacagga
     3421 gatgtaactc aaacagattt accacaaaat aagaagagtg gtctaataca agctacagag
     3481 atccttaaag gtgttccagg tattgggtct ataatgctaa cagatagaga tgttgtaaga
     3541 catgagttgg tacagagaat aataagagct tatgagaagc acgacaaaag agaagaattt
     3601 aaaaaagaag aacgtaaaaa agtgaaaatg caggaaaaga ctttcaaaag aaagtaaaag
     3661 tgtagtaata tagatatata gcattattaa tgaataaaaa gtaggtgtag tgaatggatt
     3721 tgatactaga tgatagacaa gataaattag aagtaagtga ggagttaatt gaaaagataa
     3781 aggatataat catagaatgt ttagattatg aaggatatga tgataattat gaagtgagtc
     3841 tttcttttgt agataataaa gagattcatg aattaaatag agaatataga ggagttgata
     3901 gagtgactga tgttttatca tttcctcttt taagtgatga ttttgaggat gttgagttag
     3961 aagaagaatc cctaggtgac atagtagtat ctcttgaaag agccttagaa cagagtatag
     4021 agtataacca tagttttgaa agagaagtat gttttttaat atgtcatagt atgtttcatc
     4081 ttttaggtta tgaccacgat acagatgaaa atacaaaaga aatgagagaa aaagaagaac
     4141 acatattaaa taagttaaac ataacaaggg agtagttttt atgaaaccag aaaaaactcg
     4201 tcaaggtata attaaagcat ttaatgcagc tatagaaggg atattgtata catttaaatt
     4261 tgaaagaaat atgaaaatac attatttagg ttcagttgct gttcttataa taagtttgtt
     4321 ttttaacttt tcaaaattag aaatgataat gttacttatg tccatatgct tagttgtagt
     4381 tgccgaaatg tttaatacag ctatagaaaa agctgttgat ttagtaactg atgaatatca
     4441 tgttcttgct aagatagcaa aagatgttgc agctggaggc gttctagtgg cagcactaaa
     4501 ttcagtagta gttggatata ttttgtttta tgataaattg acagatatat ctggaatatt
     4561 aatttataaa ataagagaat cagaattaca tataaccttg atatgtatat tattggttct
     4621 tatagctgtt gtagttgtaa aagcattgac ttctacaggt actcctctaa aaggaggaat
     4681 gccaagtggt catgctgcct tagcatttgc tatagcaact gcaattacac ttatgactga
     4741 aagagttgtt gcctctactc ttgcatatat aatggctgta ttagttgccc aaagtagaat
     4801 tgaaggaaaa atacatacct tttgggagac tattgctgga gctttattag gtgtgttgat
     4861 agctatatta gtatttcagc ttggaatgtt ctataattaa aataaatgta cataaaatat
     4921 ttaaaataaa acaaatctgt gctaattaaa tttttatgat ataatgtaga gtattaaata
     4981 aaaataactt ttaattattg tatacaggag gagttgttgt ggataacata gaattgttaa
     5041 ggttggcaga agatgccaga caacattctt atgctcctta ctcaggtttt agagtaggtg
     5101 cagctctttt aactaagagt ggtaaagttt atactggatg taatatagaa tgtgcatcat
     5161 taggtggaac aaattgtgca gaaagaacag ctatttttaa agcaatatca gagggtgata
     5221 aggatatcta taaaatagct atagcaagtg ataactcaga aaataatgag caaacatatc
     5281 catgtggaat atgcaggcag gtaattatag agtttggttc tgatataaaa ataataacgg
     5341 gatatactaa aggagaggtg tttgagcata gtataaagga tttgttgcca aactatttct
     5401 ctggaaatga tttaaaataa atttaaaagt ataaataaat ttaagtaatt aaaacaagaa
     5461 gataaaaagt taggagacag ttaatgttca aatcaggttt tgtcagtata gtgggtagac
     5521 caaatgtagg aaaatctact ttaatgaata atgtagttgg agaaaaaata gctataatga
     5581 gtgataagcc tcaaactaca agaaatacaa tacaagcagt atatacagat gaagaaatgc
     5641 agatagtatt cttagataca cccggtattc acaaaccaaa aaataaatta ggtgaattta
     5701 tggtaaaggc agcaacagaa gcttttaaaa atgtagattt aatattattt gtagttgatg
     5761 attctaaaaa aataggtcct ggagatagaa agataataga ggatttaaga agtgttaaga
     5821 caccaataat tttagttgta aacaaaatag accaactaga ccaaaaagat gagctatttg
     5881 atataatcaa aatgtatgat agagaaggta tttttaaaga aatagtacca atatcagcat
     5941 taaaaggaaa gaatacggat accttaataa aagttataca aaattattta gaagaagggc
     6001 caaagtattt tccagattat atgattacag accagccaga aagagtatta atagcagaac
     6061 ttataagaga aaaagttctt cactatctta atgatgaaat acctcatggt gtagctgttg
     6121 aaattgaaaa aatgaaagcg agaaatgata aggaaatagt agatgtatct gctgtaatat
     6181 actgtgaaag agattcacat aagggaataa taataggaaa aaatggtagg aaactaaaag
     6241 gaataggtaa gtcagcaaga caagatatag aattgctttt aggttcacaa ataaatcttc
     6301 aattatgggt taaagtcaaa gaaaattgga gaaatttaca aaattatata aataattttg
     6361 gatataatga taaataaagg tgggttatat ggataggaaa ctggatgctt cccaagattt
     6421 attatatgaa gtaaaatcat taattgataa taataaagta ttggagttaa gagaattaat
     6481 agaggaatat catataattg atatattcga tataatggaa aatttagagg aagacatgaa
     6541 aattcaatta tttgaagtac ttcctttaga tatggcatct tctatattag aagaaggtag
     6601 tgtagagttt tttattagta ttttatctaa actggatgta gaacactcga aaaatatact
     6661 tgaacttatg tctcttggag atatggcaga caagttgagt gaacttgaag aagaagaaag
     6721 agaacacata ataaatcttt tgaatcaaga gaatgccgat tatgtaaaag agttactatt
     6781 ctatgatgaa gattctgctg gtggaacaat gaccactgga tatatttcta taaataaaga
     6841 tatgacagct cttgaggcta tagaccatat gagagaagaa gctgaagaag ctgaaactat
     6901 ttattatata tatgtggttg atgatgaaga aaaactagtt ggagtattgt cattaagaga
     6961 attaataata gctagagacg caaatatagt agaagatttg atgagtgaaa atataatctc
     7021 tgtttatgta gatgaagata gagaagaagc agtaagatta gtatctaaat acaatttaat
     7081 agcaattcca gtagtagaca gacaagaaaa attaaaagga ataataacag tagatgatat
     7141 aattgatgta atggaagaag aagcaactga agatatgtat aaatttgcag gaagttcaga
     7201 acatgaaaga gaagttgctg aaaaagaaaa ccctacattg agagagcaaa taatatcagc
     7261 acttagagga cgattacctt ggttaattat taccttggtt ggagggttgt tggcttcgtt
     7321 aatactttct aacttagact atataatgaa tcctgtatat gctccattag tattttttat
     7381 acctgtagta attggtatgg gtggaaatat tggaactcaa tcatcatctg ttacagtaat
     7441 aacattatct aataaggatt taaattttag caatgtggtt agagaaggta ttgtaggtat
     7501 tattacaggt cttttgtgta gtataataac aggtattgta atttactttg ttatgagaaa
     7561 gttagacatt gtattaattg tttcaatatc attatttata aatatggttt taggtgcaac
     7621 aataggtgcg tttatgcctg ttttattaaa aaaaatggat gcagacccat ctacagtttc
     7681 atcaccaatt atatctacag ctcttgatat aactggtata gcagtttact ttataataac
     7741 aacggcatta ttgtcaaaaa ttgtttaata actttatgta tgatttatcc tcttcttact
     7801 aaaaatataa gtataaatgt aagaaggggg ttttttaaaa atgaacaata tcagttggga
     7861 agtttttaaa aaaacaggta gtatagatgc atatttatat ttctgtgatt gcaaaaacat
     7921 acatgaagaa attgaagaag taagggagaa aaaagaagac gatgataatc ctgaacaccc
     7981 aggggatagt acttaaagct ataaggtata aggaaagtga cataatactt acactattta
     8041 caagaaagct tggaaaagta tctgcaatag caaaaggagc taaaaaaaat aagagctctt
     8101 tgctttcatc atcacaatta ttttcttata gcaattttac cttaaaaaaa caaggtaata
     8161 tgtacaaggt tactcaaagt gaaataataa aaagttttta taatatatcc tatgatatag
     8221 aggcattttc ttatgctaca tatattacaa aattagttga aaattctata cttgaaaatc
     8281 agactaataa taggctattt atattacttg cgcagactct gtatttgtat acacaggata
     8341 atacagacaa taggtttata acagcagcat tcgagttaaa atttttagac tatataggat
     8401 ttaaacctat agtaaataaa tgcattaatt gtgaatgtaa aattttacaa aattctgtgt
     8461 ttaatatcta cgaaggtggt atactatgta gtaagtgtag taaacttttt gataataata
     8521 taaaattaga cttaacaact ataagtctta tggaatatgt attaagaaat gatattttaa
     8581 catgtagtaa agctaaagta tcaaaatata taacacatga acttgagaat attctagaca
     8641 agtatttgaa agtttatgtt gataatatta attttaaatc attacacgta ttgcaaagtg
     8701 taaaaaataa taagggagtg gataatgatg agtagtaata taactattga aatggttgat
     8761 tcagtctttg aaagagttcc aggagcaact tatgcagaag caaaagaagc attagtaatg
     8821 tgcaatggag atgtaattga ggcagtaata tatttagaaa ataaaaaaaa tacttgtaat
     8881 tgtaaagcaa aatctgctaa agaaactatg gaagaagtat ttggcaaaga tggagaaaat
     8941 ataaagtttc aattaaaaga gcttcttaga aaaagtagtg tagtgagaat aatagttgaa
     9001 aaagatggaa aaactatgat gaacattcct ttaacagttg gagttgttgg attagctttt
     9061 gtaccattag ctactttagt tggattatca gctgctgtta taagtaaata tcgtataaaa
     9121 gttcaaaatg aagaagatgg agaaattgta gatttaggtg aattgaatga agaaaaactt
     9181 cacgttttga agaatatgat aactaatgca gcaaaagatg tgaaggatgt agtagttgac
     9241 aaaaaagaag atgacaaaga tgtaactgct gaccttatga aagaagaatc tgaaaagaat
     9301 aaagaagacg aagacgaaaa taaatagata taaagtgact tttaaaaaat gaagagaaga
     9361 taattataag aattatcact aacattgaca aattttatag aatttgatat tctaaaagtt
     9421 gtagaagaac tattaaaata gcctttcgtg tagacgagag gctttattgt tataggaggg
     9481 atttttatga attttcaaga gatgatactg gcgttgcaaa aatactggtc aaagcaaggt
     9541 tgtatcatga tgcaacctta tgatattgaa aaaggtgcag gaactatgaa tcctaatact
     9601 tttttaagat cattaggacc tgagccttgg caagtatgct atgtagagcc ttcaagaaga
     9661 ccagcagatg gaagatatgg tgaaaaccca aacagactat atcaacatca tcaattccaa
     9721 gttatactaa aaccttctcc agataatata caagaattat atttggaaag tttaaaagag
     9781 attggaattg acccaagtga gcatgacata agatttgttg aagataactg ggaagctgct
     9841 acagttggtg cttggggact tggatgggaa gtttggctag atggtatgga aataactcaa
     9901 tttacgtatt ttcaacaagt aggtaatatt gaatgtgagc ttgagacagg agaaattaca
     9961 tatggattag aaagattagc tatgtacatt caagaagttg atagtgttta tgatttaaaa
    10021 tggaatgata aaataactta tggtgaagta tttaataaag ctgagtatga aaactcaatg
    10081 tacgcatttg aattatgtga tgcagatatg ttgtttaatc tgtttgatat atatgaaaaa
    10141 gaagcattaa gacttatgga aaatggcctt gtaatacctt catatgacta tgtgttaaaa
    10201 tgctctcatg catttaatac attggacgca agaggtgcaa taggagttag tcaaagagct
    10261 tcatttatag gtagagttag aaatatggca aagactgttg cagaaacttt tgtaaaacaa
    10321 agagaagaaa tgggattccc tcttttaaag gacggtgaca aataatgaaa aattatcttt
    10381 tatttgaaat cggtgttgaa gaaatgccat caagatttgt aggcagtaca ctagaacaat
    10441 taaaaaacaa tcttagtaaa ttatttgatg aaaatagaat agcttttgat aaaataaatg
    10501 cgtatgggac accaagaaga cttactttag tagtcgaagg attgagtgat agacaaagtg
    10561 atttagaaga ggaaataaag ggtcctgcta agaaaattgc tgttgatgca gaaaataatt
    10621 taacaaaacc agcattagga tttataaaaa gcaaaggatt aagcgaatct gacatatatt
    10681 ttaaacaagt tggaaaagaa gaatatatat ttgcgactat aaagcaagat ggaaaagaaa
    10741 cttcagaagt actaaaagac atattaccag agactataaa atctgtagta ttcccaaaag
    10801 ctatgcgttg gggaggaaaa aatcttaaat ttgcaagacc tataagatgg atagttgcat
    10861 tgttaaatga taaagtatta gagtttgatt tagaaggaat aatatcttct aatgtaacaa
    10921 aaggtcatag atttttaggt gaaagtacaa tagaagttga ttctatagaa gattattttg
    10981 aaaaattaga aaaaaattat atagttttag accaaaacaa gagaaaagaa atgataagaa
    11041 aacaatgtat agaagtagca aattctttag gtggagaagt tgaatttgat gaagatttac
    11101 ttgaagaggt aacttattta gttgaatatc caactgcttt ttatggagaa tttgatgtgg
    11161 attatataaa actaccaaaa gaagttgtta taactcctat gaaacaacat caaagatatt
    11221 ttccagtact taaagaagga aaactgttac ctaactttat agctgttaga aatggtgatt
    11281 cttacagaat agacaatgtt aaagctggaa atgaaaaagt tttagaagca agacttgcag
    11341 atgctttatt tttctataga gaagatacta agagaaactt agaaagctac atagaaaaat
    11401 taaaaactgt tgtattccaa gtaaaactag gaactattta cgacaaaact ttaagacttg
    11461 aaaaattaag tgctgatata cttgatagtt taggtttagt agatgaaaag gcagatacta
    11521 taagggctgc taaattatct aaggcagatt tagttactgg aatggtagga gaatttgatg
    11581 aattacaagg ttttatggga aaagaatacg caaaagttgg aggagagaat gatgttgtaa
    11641 gtgaagctat ttttgaacat tatcttccaa gatttgcagg agatatactt cctaagacta
    11701 atgcaggggt tgccttatca atagctgata agttggattc aatagcaggt ttctttgcta
    11761 taggaataca accaacaggt tctcaagacc catatgcttt aagaagacaa gctttaggta
    11821 tacttagtat acttatggac agaaaagttg ctgtggatat aaaagtactt attgaacatg
    11881 ctttagataa ttattctgaa ttagatttta ataaagaaga agttgttgag caaataatga
    11941 atttcttcaa tgagagagta aaaaatctat ttaaagattt aggaataaga tatgatgttg
    12001 ttgatgctgt tttaagttct gatataaatg atgtttctga tatgcatatg agagctttag
    12061 agcttaataa ttggctagaa aaagatgaat tagtggagat gcttactgca tttaatagag
    12121 tttctactct agctcaaaaa gcagaaagtg ataaaattga tgaatcttta ttaaaagaag
    12181 atgctgaaat tagactttac aatgaattta aacaagtaaa ggtaaaagtt aatgaattat
    12241 taaaggataa gaaatatggt attgctttag atgaatttgc atctttaaga ccagtagttg
    12301 ataatttatt tgatacagtt atggttatgg ataatgatga agctattaag aacaatagac
    12361 ttgctttact taaacaagta tatgatacta tgctagaaat ttgtgattta tctaaaattg
    12421 tttataaata aaatttaaac tatttataag aaataaggct gtctcaaaat agagatagtc
    12481 ttatttttca ttgttgagat gatgaaaata tatttgattt catagaaaaa gagtgtctca
    12541 taaactaaaa agttcatttt gagacaccca tgctttttgt aagtaaatat aataaattat
    12601 attttatact tatcagttat tttgtttaaa atattatcat ttgaatttac ccattcataa
    12661 aaattgcctg atgatgcgtc cgatatttta taaagcattt caggaatatc actagcttta
    12721 aaatctccaa gtgattttcc caatctagtc ttacctattt caaaactgcc tccaacaaat
    12781 aattcaaatg catctgtaga tataccatca acattcttct ttttaccaca taaacctatg
    12841 ctaccaactt gatgaacacc acatgaattt aaacatcctg atatatataa cttaggtgct
    12901 ttatttaaaa tttcttcatt attttgtaat ctaaaataat ctatgatttc atgtaacatt
    12961 ttctgactat tttgtatgcc catttgacaa attggaacac ctatgcaaga tatactttgt
    13021 tctaattgag aattacaact aatagtttta cttatttcta atattctttt tgcttcattt
    13081 ccatttaaat ttaaaatata taatccctct gtcattccta gtctaatcat aggattttca
    13141 acattatcta attctttaag taaagtactt aaatcctttg ttaacaaaat acctccaact
    13201 ggatgtatat atacagtgta taaaccatcc tgtttttgag gtattagtct agaatcttgt
    13261 atatctattt taactccagg tttagagtaa tctatttctt caatattgag ttcaagacta
    13321 ccattttctt tttctaattt atagtattct ttaaattttt ctaagaatag ttcttcacca
    13381 agtgattcga ccatatatct aaccctagct ctgtttttat tttcataatt tccataatcc
    13441 ataaagaatt taatcatacc ttcaacacaa tataaggcgt ctttcggctt tataagttca
    13501 tcaagttcta aagcaacttt gggttgttta cctagacctc cacctaagaa aactctgaaa
    13561 tatggttcat catttttcaa agtagcaaca aatcctaagt cttgtatact acaatgagca
    13621 gtatctgtgt aacaattaga ataagaaact tttaattttc taggtaaatg gtatgtagta
    13681 gcatttttga taaaatattt atcagtagca actgcatatg gagtaacatc aaagacttca
    13741 ctagggtcaa ctccagataa tggagataat ccgacatttc taggataatt accaccacca
    13801 cctctagtga atatatcatt ttttattcct tcttccatga tatcaacaat tgaatttata
    13861 gataaatcgt gtaattgtat agcttgtcta gttgttaagt gtatcttatc taagttgtgt
    13921 ttaatagcta tttcatagat tttattaagt tgatactgag ataaaactcc agcagatact
    13981 ctaagtctta tcataaaaga ttttttgtct ctttgagcat aaacaccata tccaccagaa
    14041 aatcccttat attgcatttt atttatttct ccatttaaaa attttgaaga atattcttta
    14101 aaattaggta gttcatttaa caatatcttt ttaaaattat ccattatttc acctcattgt
    14161 tagtcttgta taaaaatata tctattgtat aatagatata tatattatta tctagtcagg
    14221 ataaaaatat aacagaataa ttctattaaa agtaagaata ataaggacat agtgagtatt
    14281 ttgtttttat tttacaaatt aagtataata tttttgtttt ataaaatata attaagataa
    14341 atagataatt ttgtaatttt aatatataat atttagtaga gtgtgggagt agcacttaat
    14401 atttgcagga ggagtttttg tatatgggag aaataataaa tgcattagat agagccctag
    14461 acataatttt gctgttatat catgaaaaga gagaaatggg aataactgaa ataagtaagg
    14521 ctatgggagt atataaaagt actgtacata gaactttggt tactcttgaa aacaaaggtt
    14581 ttgttataca aaatgcagag aatggtaagt attggctagg aataaattta tatgcaatag
    14641 gtatggttgt gggagaaaaa atgtcattga ctgaaatagt aaaaccatat acgaaaaaat
    14701 taaatcaaga atttaacgaa gtagttaatg tatctatatt agaagaaaga gctcaagatt
    14761 ctccaagaag tatcataata cataaagagt atggttcaaa tcaattactt tcagtaaatc
    14821 catccgttgg ttcaagtagt gaatgttatt gttcagcagt tggtaaatgt cttatggcat
    14881 ttaatgattc tatagatttt gaaaaatata gaaaaacacc aatacacaag tatacagaac
    14941 atacaattga taattgggat gatatgatgc tttttttagc aaaaataaaa gaacaaggat
    15001 atgctataga tgatgaagaa ctagaacatg gactaacttg tattggagca cctatcttag
    15061 ataaaaataa taaagctatt gcagccataa gtttgtcagg tccaactata agaatgagag
    15121 aaggggactt tgagtataag attaaaagag ttatagaaac tgcaaaaagt atatctgaac
    15181 tttttagatg atttttaatc tcgataataa aaataggcta tatttaaatt gttatttgta
    15241 aatatcagtt taaatgtagc ctatttttct ttaatctaaa attaaatatt cttaaaattt
    15301 aaaatgttat tgaaatatat aagcgaatgt gttatctttt aattaagcaa taaaaataga
    15361 acaaggttct atactataga acaaaattga tgcatatata gtatttatac aaaatataaa
    15421 tattatatat ttaaaattaa gtaaaaatat tacataaagg ggagatttga gaaatggaaa
    15481 aagtcaagtt gagtgttgaa agatgtaagg gatgctatct ttgtattgaa gcttgcaaaa
    15541 aagatgcaat aagtctttct gactacacaa ataaaaaagg ctttgtaact attaaagtcg
    15601 atgaagaaaa atgtattcaa tgtggcgctt gttatacaat gtgccctgat ttagtatttg
    15661 agatattata aattattttt attagagggg agaaaaggta tgcaaagttt attaattaaa
    15721 ggaaatgaag ctttagcaga aggtgctata cgtggtggct gcagatttta tgcaggttat
    15781 ccaataacac cacaatcaga aatattagaa atgatggcat ggagacaaaa agaagttgga
    15841 ggagtattta tccaaggtga atcagaaata gccagtgtaa atatggttat gggagcaagt
    15901 gcacttggag caagagctat gacttcttca tctggtccag gattttcatt aaaacaagaa
    15961 ggtattgcat attgggtatc agctggtttg ccagcagttg tagcatgtgt gcaaagatat
    16021 ggtataggtg atggatttat aggagctggt caagataatt attggcaagc agtaaaaggc
    16081 ggtggaaatg gagattatca tttaatcgtg ttagcgccta acagtgttca agaaaatatg
    16141 gatatggcat atgaatcttt tgaattggct gaaaaatata tgcatccagt acttatactt
    16201 tctgatgcta caataggtca aatggtagaa ccttgtatag caccaccaat gaaagaatac
    16261 gatatggata aagctccatg ggctgcaaaa ggaacacctg taggagaaaa aaataaagta
    16321 ataactgata taacatattt tgaagaccaa gatacatggc aaaaagggta tatagaaaaa
    16381 atgagaaaag ttcaagaaga tgaacaaaga tgggaagaga tagaaataga agatgctgaa
    16441 gtggtatttg tggcatacgg tatttcatca agaatagcag aaggtgcagt tagagatgct
    16501 agagcaaaag gaatgaaatt aggtctaata agaccaaaaa cattgtggcc ttacccaaga
    16561 aaagcattta agaaattaag tgattcattg aagggacttg taacagttga aatgaacatg
    16621 atggcacaga tgaaggaaga tgtaattata gcatgtgata atagatttcc agtatattca
    16681 gtagcaacag ctcaatatat ggcagaagta aatcaattaa tagattttgc tcaacagatt
    16741 atagatggaa aagctaagca agaggaggta ttttaattat ggctaagaaa gcaccagcaa
    16801 taatgatgtg tgaaaatatg ttttgtggag gctgtggaca ctctatagtc gatagaatta
    16861 ttggagaagt attaacagaa atggatatat gtcaagatac aattatatgt tcagatatag
    16921 gttgtaatca tatgtttcaa tttagtatga atgtagatgt tgtcgttcca cctcatggta
    16981 aaatgggtgc agcaatggca gctatgaaaa gagttagacc agataaatat atatttacaa
    17041 tagctggaga tggtggagct tatgcgatag ggcttgggga aactatgtca gctgctacta
    17101 gaaatgataa tgttatattc cttgttatga acaataatat gtttgcaatg acaggagggc
    17161 aaatggctcc aactactata gaaggtcaat atacagcttc tactccagcg ggtagaaatt
    17221 acgcagatca tggttcaaca tttgatgttg taaaagttat gggaaattta gatatagcat
    17281 atctagcaag aggaacaata actaacccta aagaaataaa tacaacaaaa agatacatca
    17341 aaaaagctct agaaaaacaa aaagaaaaga aaggtttttg cttaatagaa gtgcttgtac
    17401 catgtccaac aaactgggga ttaaagccag tagatgctat gaagagaatt gaaaatgaac
    17461 ttgttccaaa gtatccactt ggagaattta ttgattaggg aggaaataat atgttgaatg
    17521 aaattatatg tgctggtttt ggaggacagg gagttcttac agcaggaaaa attataatgt
    17581 atgttgcata taaggcagaa aagaaaatta catggttccc atcttatgga aatgagatga
    17641 ggggaggaag tgcaaattgc aatgttgttg taagtgatga aaaaattgca aatccttgtg
    17701 taaaaaatcc agatatacta ttgggaatga acagcgtatc agtagataag tatgaaagtt
    17761 taatgaaaaa aaatggttat atgtttgtca atgaatcact agtaagtaag gatagaaagt
    17821 acagagaaga cattaatgtt ataaaagtac ctgttactga aatagcacaa aatttaggca
    17881 atgaaaaagc tgctaacatt tgcatgttag gtgctatggt aaagagtttg gatttattta
    17941 caaaagaaga atttgaaagt ggtatgtgtg aatactttga aaatcaaggc aaaggtaaat
    18001 tcaattctaa aaatgtagaa gcatttgaag caggatacga ctacgaatga atattttaaa
    18061 tatttaaaat aattattgaa atacttattt ttatatgtta gtatttaatt aataaaatat
    18121 agaacgtcgt tctggacaat aggacgaagt ctttaggccg gaatacaaga acgacgttct
    18181 gtatagtaga actagggggt gttaaattga aagttttagt aataaatcct ggtggtacat
    18241 ccacaaaaat ttctgttttc caagatgaaa atgaaatttt gaaaaaaaat ataacccata
    18301 caagagaaga tttaaaaaat ttttctaaag tatttgatga atatgattat agaaaacaac
    18361 tgatagtaga tattttaagt agtgagaatc atagtataaa ttcttttgac gcagtagttg
    18421 gtcgtggtgg acttatgaag gctataaaag gtggtaccta tactgtgagt gaagaaatga
    18481 ttgaggatat gagaaatgaa ataaatgggg aacatgcatc aaatttaggt gcattattag
    18541 ctaaaactat agctgatgaa ataggagtac aatcatttgt agtagaccca gtttctgtag
    18601 atgaatttga tgatgtatcg aggattacag gaatatcaga catagaaaaa gcaagttggt
    18661 tacatgcatt aaaccataaa gctgtttcaa gaaaaattgc acaaaaacta gggggaaaat
    18721 acgaggatta taactttatt gtagctcatt taggttcagg aatatcgatt gtagcacata
    18781 aacaaggcaa aatgattgat ggaagtggtg gtaggtctga tggtccattt tctccagaaa
    18841 ggagtggtgg attacttgca tatcctctta tagaattgtg ttactccgga aaatatactc
    18901 atgatgaaat ggtcgcaaaa gtaagttcga ttgggggaat gtacgattat ttaggaacaa
    18961 aggacatgat agaaattgaa aacagaatca aggagggtga tgaaaaggca aaacttataa
    19021 tggatgcatt tatttatcaa gtagcaaaag aaatttctat gtatggagct tcactttcag
    19081 ggaatgtaga tagagttatc ttgacaggag ggatatctca ctcagaaatg gtagtcaaag
    19141 gagtagcaga aaaagtttca tatctagctc caatagagat agtaccagga gagatggaaa
    19201 tggaggcact tgctctagga gcacttagag tacttaatgg agaagaaata gcaaaaactt
    19261 attaatattt aggagaaaga gtatgttaaa agattttaat aatttattag aagaggctaa
    19321 aaagcttgaa cctgttattg taagtgttgc tgttgcagaa gacaaagaag tattaaaggc
    19381 tgtgaaatca gcaacagatt taaacattat aaagccaatt cttgtaggag acaaggataa
    19441 aatagaaagt atatcaagag aaattggatt aaaaggtcat gaaataatag gttgtcaaaa
    19501 tcccgatgaa tcagttaaaa aagctgtaga gatagttaaa aataaggaag ctaaagttct
    19561 tatgaaggga ttagtaaata caagtgtcta tatgagagct atattaaata aagaaacggg
    19621 tcttagaaca ggaagattat taagtttgct tgcagtatat gaattacctc aatatcataa
    19681 acttctttat tgtactgata gtggaataaa tgtatcgcca aacttaaatc aaaaaaaaga
    19741 tataatgaca aatgcacttc ttactatgaa aagtataggc atggaaaatc ctaaaactgc
    19801 gatacttact gcaaatgaaa tggtagaccc taagatacaa tctacagtgg atgccaacct
    19861 acttgtagag atggaaaaat cgggagaaat accaaagtgt atagtggaag gtccaatagc
    19921 atttgatgta gcatttgatg cacatgcagc agaacataaa ggtattaaaa gtaatattgc
    19981 tggtgatgtt gatttattag tatttccaaa tatagaagca gggaatatat taggaaagtc
    20041 atggcttaga tttaataaag ctaagtgggc aggtatagtt ttaggagcaa caaatccgat
    20101 aattttaggt tcacgttctg acacagctga aataaaaata aattcaatag cattagcgtg
    20161 cctagcatca caataagaaa ttattattaa ggaggagaaa gtattatgaa atatgcaaaa
    20221 agaatggata tggtaaaagc ttcagctata agagcttctc aaaagaaaat agctcagaag
    20281 gtggcaagtg gtggaactgt tatatctttt gctgcagggc ttcctgaccc aaatttattt
    20341 ccaatagaag agataagtga aataactcaa aaagtactta atgaaagggg aaaatttgcc
    20401 ctagcatatg gacctactaa aggtgaagat gagttgttag atctattagt aaaaagaatg
    20461 aaagaagaag aaaatattga tacaaaagca gaaaatatta taataactac tgggtcacaa
    20521 caagggatag cattaagtgc tatgatttta ctagaaaaag gagatatagt agttacagaa
    20581 aatccaagtt acttaggtgc aataaatgca tttagacctt atgaatgtga ttttttagga
    20641 gtagatactg atgatgaagg aatggtaaca gatgatttag ataaattatt atctgaaaat
    20701 tctaacataa aggtaatcta tgtaatacca acatttcaaa atcctacagg aaaaacatgg
    20761 tctttacaaa gaagaaaaga ttttatggaa gtcgtgaata agtatgatgt catagtaata
    20821 gaagataatc cttatggaga aataagattt acaccagagc ctctacctac acttaaatct
    20881 cttgatacta aagataaagt attatactta ggttcatttt caaaagtatt atgtccaggg
    20941 tttagagttg cttggatgtg tggtaatgta gacatcatag ataaggcaga acttttaaaa
    21001 caaggtatag atttacagtc aaatcaattt tcgcaagtac aagtaataga atttttaaag
    21061 aaatacaact taaatgaaca tatagaaaaa ataagagcag agtataagaa aagatgtctg
    21121 ctaatgttag atgctatgaa gaaacatttc ccagaggaag ctaaatatac agaaccagat
    21181 ggaggaatgt ttatatgggt tgaacttcct gaaagtataa atgtagatga tttattggat
    21241 aaagccattg atgctggagt tgcttatgtt tctggagagt ccttctttgc aaataatggt
    21301 ccaaaaaata ctatgagact taactataca acaatgtcag aagaacaaat cgttaaaggt
    21361 gttgagatac ttgcagaagt tataaaaaaa gaactcaatt atgcttgtga ataaatataa
    21421 aaagtgaggg tttaaatatg ggagaaaaag aaaacggtat aaagtttgga aagttaatgt
    21481 ttcaacttgt tattatagca atatcttggg aattagttta tattatacca tttatccaat
    21541 atacattata tgatccaatc ctaaaagcat tagaatgtac aaacactcaa ctaggttttc
    21601 tacttacaat ttatgggtta ggtaatatat ttggagctcc tataggaggc tggttagcag
    21661 atagatttga ttatagaaag ataattttag gttctgtatt tcttaatgga gtagtatcat
    21721 ttttatttgc atttaatatg aactatccat ttgcagttgc tacttgggta ggttgtgcaa
    21781 tcacatcatt agttatgaac tatccttcta tggtaaaaat acttagagtt ataggaaaag
    21841 ataatcaagg taaagtttat ggattcaatg aagctatggt tggagtttct ggcgtagtaa
    21901 tgggagcaat attcttatat atttatacat gcttttcaac tcctgtattg ggtatgagat
    21961 gggtaatgat atctttagga atattatcaa tagtgatgtg tccagtatta tggtttgtaa
    22021 ttaaagatgt agatatcaga gaagacaaag aagaagaagc aagtgaaaaa atgtctgctg
    22081 gcgattttat gacagtacta aaatcaccaa acacttggct agtaggaata agcatattct
    22141 gtgtatattc attcacagtt acaatgtctt attttacacc atatataaca tcagtacttg
    22201 gaggttctgt ggcattgtct ggggcattag caataataag acaacatggt ctaaaattgt
    22261 ttggagcacc atttggagga tattgtgcag ataaagtaaa atcacctaca aaagtactac
    22321 taccaatata tgtatttggt atagcagtaa taattttatt cctagtacta cctgcatcta
    22381 ctccaatgac tatctttata gctttaacat ttgtagtagg tatattaggt tacatgggaa
    22441 aaggtatata ctatgcagtt caagatgaag taaaagttcc agttaaatat tcagcaacta
    22501 ctataggtat agctgcagct ttaggatttt caccagatgt attccaattt gcattaatag
    22561 gacactggat agatacttat ggcaataaag gttacacata tacatttata ttccagatag
    22621 caattctaat aattggtata ctatcttgtt tatatatatt aaaagttaag aagaaaagag
    22681 aaagtaagtt agaagcataa atattgtaaa aataccaaat tattaagttt tatagcaata
    22741 cttaaaattt aaagtgcaac acaccaaaat gttatcaagt tttctaggtt ttagtataaa
    22801 gttaccccta aaggtctgga attgagtagc aattatcatc tatttatata tttacataaa
    22861 cctatttaat ttaaattaaa agaatttcaa aaataggtgg taattgtact caattttaca
    22921 cctcataata aatacgaaaa tttaaaatct aactatatac tattcaatta tatattaata
    22981 attgtatatg gtactattta tatagtaaca gtttcaggct tttcattaca tgtattccaa
    23041 tttatattgt ttgattattt gtcaaataca tatcgttaaa aagtttataa atttacgttt
    23101 atatatcgat taatattctt tgttttagag atagatactt atagcttgtg gattaaaaat
    23161 aatataaata gaagatagat tttttagata tattgagttg aagattatat agaatgttga
    23221 aacatagcta tttaaattat tgtctataaa tataaaatga atggtggtga gttaagtgac
    23281 cgatttagtt aacaccatta atggaattgt ttggtcacct gtattagttg caatgtgttt
    23341 gtgtatagga ttatattttt caatgaaatt aagatttttt caaataaaag atgtaaaaga
    23401 aatgtttact cttttgcttg aaggagatag ctctcatgtt ggaatatctt cgttccaagg
    23461 ttttgctaca tctatggcag gaaggatagg aactggaaat atagctggtg tagctgtagc
    23521 gatagcaatg ggagggccag gagcattagt gtggatgtgc ataatggcta cattagggtc
    23581 ggcaactgca tttgtagaat ctactttagc tcagatatat aaagatgagc atgatggaca
    23641 gtacagggga ggtcctccat attactttga aaaaggtctt ggttggaaaa aatgttcaat
    23701 tttattttca gtggttgcaa tcataagtta tacatttttt atgcctggca cacaatcaaa
    23761 tactctatat ctagcaatga atcaagcatt tggattttca aaaatgacaa tagcagttac
    23821 atcttctatt gcactaggta taataatttt tggaggaatt aaaagaattg gtttatttgc
    23881 tgagaaagta gtacctataa tgggtggagc ataccttatt atgacattgg taataatcat
    23941 agcaaatata aatctagttc caggagctat tatgacaatg tttaaatctg catttggatt
    24001 tggttcggtt tttggaggga tgttaggttc agcaatatct tggggagtaa aaagaggtat
    24061 atactcaaat gaagcgggag aaggttctgg aacttatggt gcagcagcag cagaagtgag
    24121 tcacccagcc aaacaaggat tagtccaagc tttttctgtt tatgtagaca cactattaat
    24181 atgtatggct acaggtatta tgattataat aacaggaatg tataatgtta cagatgttta
    24241 cacaacatta ccaggtgtag aaccaggtcc acaatatgta cagagtgcaa taaatacatt
    24301 acatcctcaa ttaggtgata tttttatagc aatagtttta ctactattta catttacaac
    24361 attattagcc ttctcataca tgatggagac caacgtatct tacttagtga ctagaattaa
    24421 ggcagaaaaa catgaaaagc acataattaa tgtctgtaga tttatgctta taatatgtgc
    24481 agcaatgtgt tgctttatag aaccacttac aagttgggca cttggtgata tatgtatagg
    24541 tgtattaaca tatataaact tagttggtat atttacacta aaaaaaccag ctataaaagc
    24601 atattacgat tatcaaaggc agaaaaagat gggagtaaaa aggagcgata gaacttttaa
    24661 tcctatagaa ttagatataa gaaatgctga gttttgggaa aatagatact taaaagagga
    24721 taagcatgag ttaaattaaa gttataataa aatataaaaa ttactaaata aatttagtgg
    24781 gaggtaagat tggtatgaaa gaaagaataa tacaagtggc tgattcaaag aaagaaaaaa
    24841 ttctagggct atgtcaatct ttatatgatg aaccagaaat tgctttgcaa gaatataaat
    24901 ctgctaaaaa aatatcagaa tttctaagag aagagggttt tgatgttgag gaaaacttgg
    24961 caggtatggc tacagctttt aaagcaacca agaaaaatgg agatggtcct aaaatagcat
    25021 ttatagcaga atatgatgct cttccaggca atggacatgc atgtggacat catttaattg
    25081 catctatggg agttggagct ggaatagctc tttcaagtat actggatact tacaagggag
    25141 aagtaagtat aattggaaca cctgctgaag aaactggaga tggaaagcct tacttgatag
    25201 aacatggtgt atttgatggc tatgatgcag caatgatgat acatccaaat tcaaaaactt
    25261 gtgtaacacc tgaaattata gcaataggag gtctagattt catatttact ggaaaagcat
    25321 ctcatgcagg agctaagcca tataacggaa taaatgcttt agatgcagta gtactactat
    25381 ataataatat aaatgcacta agacaacaat tagtagatgg aacgagaata catggtatta
    25441 tactagaagc aggaactgct gcaaatgtaa tacctgatat gggaaaagta agattagaga
    25501 taagggcaaa agaacaaaat tactttgatg aagtagtaga aaaagtgaaa aattgtgcta
    25561 gaggtgctgc aatagccact ggatgtgaac ttgaatttta tcactttgaa ccaacttgcc
    25621 aaggattaaa tgaaaataaa gttttggtag acatatttac aaagattatg gaggaatttg
    25681 gaatatatga agatgaacaa gtaatgttag gttcaacaga tatgggaaat ttaagtcaaa
    25741 taatgccatg tatacatcct ttgatgaagt tttctgaaaa tggagaagaa ctacatacaa
    25801 aagaattttt ggaggcttct ataaattcat atgcaaaaga tagagtaatt gatggtataa
    25861 aaatattagc tttaacagga tttaatttat ttgaaaatcc agagttattg aaaaaaatga
    25921 aggaagagta gaacttagac tatttttaat tatactcttt aaaataagaa gagtgtctca
    25981 aaatgaactt tttagtttat gagacactct tttttgcatg aaattaaata tatttttatt
    26041 attttaacaa tgaaaaagag gcttatctgt ttttatataa ccttttttaa cttgtagcac
    26101 aaaatatcat gaatgattac aattcgaatg ataaaatcga ttataagata tataggaggg
    26161 gtacatatgg aaactttaga agtaagaaat attgaattga gtggtacaaa ttatgagata
    26221 ggatacagac taggagaatt ggttgctaat atgccagaga taatagaagg tcagattaat
    26281 aaatctaatc tggtaagcaa aaaagaagaa aaagagatga tagagttgtt tgataaatat
    26341 tgtccaggtc ttaatgaaga attgcaaggt tttgctgatg caattcaagt gaattgtaat
    26401 caaattttat attatactat gacttatctt aaaccaggtt gtagtcaagt tgcattaaca
    26461 ccagaattaa ctgaaaatgg gcatgtacta tttgcacgta atttcgattt ctcacacaat
    26521 atggaggatt ttgttttatg taaaactaaa gtaaatggaa aatatgcaca tataggtact
    26581 accgttatgc aatttggaag aggtgaaggt atgaatgaat gtggattagg tgtaagtcag
    26641 tcatcatgtg gtattcctgt aggcaattca gatggtttga gaaaaccagc aattgtaggt
    26701 ttacagtttt gggctgttat tagatatttg ttagaaaatt gcaaagatgt agatgaagca
    26761 ttagaatatt taaaagatat gccaattgca tacaatatta atttattgct tgcagataag
    26821 tctggaaaca ttgcacttgt ggagacttta gatgggaaaa aagaagtaaa tataatcaac
    26881 agtttagaaa gtaagagaga atatttttta cactctacta atcatacaca tattgataaa
    26941 cttcataaat tagaaccaca atcaatgaaa aattctattc atagatataa attaattaaa
    27001 gaatatataa ataaggctaa aaaaattgga gagaaagagt taatgaattt attgtcttta
    27061 aagtatccaa atggcttatc ttgtaattac tataatgatt tttttggaac tttaaaaagt
    27121 atagttatgg atttaaatat aggaaagttt aatattttat ggggtggact agagaataaa
    27181 tgggagagct actatttaaa aaatgacatt aaatctataa cacagaggat aaacataaat
    27241 attgaaaaag caccatctga cttttttgat ttcattgaat aaaaaatgta tttaattatt
    27301 ctaaataatt aggctatata aatcaactaa aagtttttat ttatcgtatt gaattagtta
    27361 taatatttta gtatagtaaa aaatagtgct attttacata tttaaagagt ttaagtagaa
    27421 gagaggtgcc atattggatt acttaaagta ttttgaaaaa gctgtaatgt atattgagaa
    27481 taatttacat gaaaatataa ctgtaaatga tgtggcaaaa gaaactggat attcttatta
    27541 tcatttaaca cgcttattta agtctatgtt tggtgagagt gtaggaagtt atataaaaaa
    27601 aagacgatta gtaagtagta caaaagaact tttatatagt gataaaaaag tgatagatat
    27661 agctattggt agtggatttg aatcttcgga agcatttagc agagcattta aatctatata
    27721 caaggttagc cctatagaat atagaaaaaa tagaattgat gtatttgttg gtaaaaagaa
    27781 aaaattagag ttagatttta tgagacatct tgttggaaat ataacgatta aaccagttat
    27841 aaaagaaata gaaaaaataa aggtaatagg tataaaggat aaagtaattt tagaaaataa
    27901 ttcattacct gacttgtggg agaaattcag aaaagtacat catattgtcc caaatacttt
    27961 gccatcaaag agagtttttg gaatctgtga agcaatctct gaaatacacc tagttagtga
    28021 aagtatggaa tttaatgaga taataggact tgaagttaat agttatgatg tgattccaaa
    28081 ttcatttgtt tcaaaaacta ttaaggaagg aaagtatgct atatttactc atactggtag
    28141 cttagataac ttagacaaaa cttatgaata tatatggggt acttggtttt taagttcaaa
    28201 agaagaactg gatataagag atgattttga agtatatgat gaacgatttt taggtcctag
    28261 taatgtgaac tcacaaattg atatttatat acctataaaa taatctataa gaaaactaaa
    28321 agataatttg attaagaaat agatgttcgt aaaagagcat ctatttttta tttttatcag
    28381 atttagataa ttttaataat aaaagaaata aaaataagtt cttaaatttc taagaagaat
    28441 ttagaatata gtcgaaataa gaattgtata tttctgaaaa ttctaattag tacaattttt
    28501 tcctttataa aattgaaaaa agatgtattt atttatcaaa aatattatat taaaatgata
    28561 ttaataaaaa aacatttaca atattagcat atagtacaga aaaaagatgt aatttgtaaa
    28621 atttatgtta agcagtaaaa ttaaaaattt aaagatatta tagggaggtt ataattttat
    28681 ggaaaacgtt attacaggtc taagtaaagg tgcagagtgg tttatcggtt tgtttcagaa
    28741 agggggagaa caatttatag gtcttgtttc aggtactctt ccaacgctaa ttgtattaat
    28801 ggtagctatt aactctttga ttaagataat tggtgaggaa agagttaata attgggcttc
    28861 taagctagga aagaactctt ttacaagata tattctactt ccattagtat cagtattttt
    28921 cttgggaaat cctatgtgct atacatttgg tagattttta aaagaagagc acaaagcagg
    28981 attttatgat gcagcagttt catttgtaca tccaattaca ggtctttttc cacatgcaaa
    29041 tgcaggagaa ctttttgtat ggttgggaat atcagcaggt ttgactactt taggtaaaga
    29101 aactactaca ctggcattgt ggtattttat agttggtcta attgtaattt ttataagagg
    29161 tatagtaaca gaaaaaatgt atgcttttct aacaagaaaa aatagagcaa caaataaggc
    29221 ttaggaggta gataaaatgg aggagaataa aatattaaaa atagaaaagg gaacttcagg
    29281 ctggggaggt cctttgtata taaaaaaaga agggaataga aataagatac tatcaatgac
    29341 tgctggtggt atccatgaag taacattaaa aataaaagaa ctactgggtt gtgaaattgt
    29401 agatggattt aaaactggag tttctgatga agaagtagca gtagttatta ttgattgtgg
    29461 tggaactgct agatgtggag tttatccaaa gaaaaaaata cctacaataa atgtaaatcc
    29521 agttggaaaa acaggtcctt tagcaaaatt tataacagaa gaatattatg tatcagatgt
    29581 taatcctaac tgtataagtg ttgtagatgg ggaagatatg ccacaaaaat ctcaagaaaa
    29641 taaaagcgaa aataagagtt ctattagaaa gcctgataat tatgatgagg ttaagtcaaa
    29701 agcacaaggt gaatatgcta aaaagaacat aatactttct attggtcaag gggcagggca
    29761 agtagtatca aaattttatg atgcaggtcg agacactatc caaatggtaa tgaataacgt
    29821 aataccattt atggcatttg taagtatgct tatgggaatt attttagcat ctggactggg
    29881 agactggatt gcaagagtta tatctccact tgcaggtaat attggtggat tattgattat
    29941 atcagtaatt tgcacacttc cattcttatc accaatacta ggacctggtg cagttatagc
    30001 tcaagtagta gggactcttg taggtacaca aattggtctt ggtgcaatac cagcatattt
    30061 agcacttcca gctttatttg caataaatgg gcaagctgga tgtgattttg taccagtagg
    30121 attaagtttg ggagaagcag aaccagaaac tgtagagtat ggtgttccag ctttatttta
    30181 ctctcgttta ataactggtc caatagctgt tattattgca tatggagttg cagtatttgc
    30241 attgagataa tatttaatgt caatataagg cttggaatta attaataaaa ttgtaaatat
    30301 aaatttaagc tagtcaagat tttttgttaa tgaaagacta gcttttatac tattaattag
    30361 ctgtaattat cgagttagat taatactaaa ttttccttta ttaaagtgaa attttatgta
    30421 ttaatttggt ttcaaatttg tactagaatg aaatcaagga ggagatacaa aatgagtgta
    30481 actgagaaaa ttccttctat tgaccgcaag atatttaaga tattgattat gtgtagtaaa
    30541 aaagagtttg tgagtattaa ttcaatagca aatgaattga atgtcacaac tagaagtgta
    30601 aggacgtata taaaacaatt gaataaagat ttaggtaatg atatagcagt aatcaaatat
    30661 ataaagggtc aaggctataa actagaaata aaagatgagc agatactcaa taagattatt
    30721 gatatgaata gaaaaaatgt attttcatta aattcaaaag aagatagagt agagtttata
    30781 ctaaattact tgatagaact agatggattt attacacttg atagtttagc tgatgagatg
    30841 tgtgtaggaa gaactacttt agtaaatgat ttccagtatg tagaaaaagt tttggcttca
    30901 tataatttaa acttgattaa aaaacagaat acaggtatga aattaaatgg aaatgaactt
    30961 gatattagac tgtttatatt aaatcagttg tataaaaact caaggaaaga tttcagtaat
    31021 tcaaagtatt ttaaaggaat caaaaaagaa gaaatcataa atttagaaga aaaactattg
    31081 actttattta aagaaaataa tttttatgtt acagatgaaa tgttaagaga agttattaac
    31141 tacatagtag ttttagtcta tagagtaaaa gaagaaaaga aagtaaaaga ctatgatgta
    31201 aagtttgatt tacttaaatc atatgatgaa tactttattg ctagggaaat aaaacatata
    31261 atatcaggaa tgtttgaatg tactttaaat gatgaagaaa ttatttattt aactatacca
    31321 ttagttagtg gaaatgcacc agcatcagaa tgtgctttaa atagtagtag aattagtaaa
    31381 aacattgatg agttgatgga aaatatattt aatcaaatat atgtcgatat gggtattttt
    31441 ataaatgagg atgaattaag agtaggtcta ggatatcatc ttagttttac tttaaacagg
    31501 ctattattta atataaagtt aaaaaatgtt ttgttagaag agattaagca aaattatata
    31561 cttccattta aattagcaca aatagcagga aaagtaatag agaaaaagta taacttagaa
    31621 gtttcagaag atgagattgg atatatagca atccatttca gtggatattt agaaagaaat
    31681 agcagtagat tctattcaat aaaaaaaata gcaattatat gtagtacagg tctaggaaca
    31741 gcaaaactat tgaaaataag agtagaaaaa ctgataggaa ataatcctaa aattgatact
    31801 ctttcaagtt ttaatttgag aaatattaac ttagatgaat acgatatagt ttttacaact
    31861 attgatttag acacatcgaa tataaataca atagtattaa aaataaatac aatatttgat
    31921 gaaaacaaat taagagaaca attaaaaaca gtattgtgcc taagagaagg gaatattgac
    31981 actactaatt ctacaaattt actaataaat aatttgttag atgaagataa atttatgata
    32041 ttaaatgaaa agactatact tgcttcatta gaaaaaatga tggaccatct tatggatttg
    32101 ggatgtattg atgataagtt tagaaaaaat atagtcaata gagaagaaaa atctcccaca
    32161 gtatttgata aaggtttatt atttcctcac tctgtaaatg aaaaatcaga taagttttta
    32221 atggcagttg gaattttaga agaaccaata gaatatgcaa atagaagtat aaaaataatt
    32281 cttatgacaa tgtttccatg tgagaataag atagactcag acttattagt aaaaatttat
    32341 gaagaagttt taaagatagg ccaagatgta aaattaacga ataagataag taaatgtaga
    32401 acttttttag aatttaaaaa ggtgttactt aaaaacctta tataactatt gtacagcatt
    32461 tatagttgtt atacagattt aaaaataatg tgatttttgg aaggtgtata aatatgaaat
    32521 tattagtaat tttatttgtt gcttatattt taaatactat atttataatg aagcagaata
    32581 aattgtattt taaaacttta aatagagcta aaaaaaaggg tgatatagtg tctacaggca
    32641 aaaagaaaag ctatttttct aaaggaagta tagccattat atcttcagat tcagatgggt
    32701 ttataaagtt tggagaaatt ttaaaagggc gtacagtaat ggctaaattt aaagagattg
    32761 aagaaataga aggattagat atatatagtg cagagcaaaa atttaaagat gaagagagta
    32821 ttgtacaagc tataaaattt ataaaagaaa aaataaatgt ttcttttaat taactcaata
    32881 taaatataaa ttatcaggag gaatgtcatg aaatatgaag ttaagataag tgggataggc
    32941 agtttagtta aagagttaat ggatgaaagc aattgtctta ttatatatga tgaaaccatt
    33001 aatgatgacg acttaaagga tatatctgta attcattcga tagcaacatt aaaaagtgat
    33061 gttgaaatag gagatacact tactataggg aatagagatt attgtatagt ttcagttggt
    33121 gatattgctc agaagactct tagagaaatt gggcattgta caatcaaatt tgatggaaaa
    33181 tgtgaagtaa atttaccagg agaaatacat gtggaaatag gaaatccaga tataactatt
    33241 ggagatttaa taactatttc ttaatttgaa aataaaatta ttataacagg aggaattggt
    33301 atgttagatt taaacagaaa acttgcaaaa ttggaagagg aaggtaaaaa aataaaagta
    33361 gctcttatag gagctgggca tatgggaaat ggaatggtaa gccaaatggc aaatatgaaa
    33421 ggtattgaag cttctattgt tgtagatata aatttggagt tggctcataa agcttttact
    33481 gatgcaggaa tagatgaaga aattatagat aatgtaacaa atgaaaagga tgcagaaata
    33541 aaattacaag aaggaaaagt attgacatgt aatgactttc ttgtggcttg taaaactaag
    33601 tcaatagatg tagttataga tgcaactggg ggtattgcaa ttggtgcaga gatagcatta
    33661 aactctattt taaataaaaa acatatagtt atgttaaatg ctgaaactga ttgtgttgtt
    33721 ggaccaatac ttaaaaaatt agcagatgat gctggagtga tatttacagg ttctgcagga
    33781 gatgagccag gtgcagtaat ggaattattt gattttgcag atgcaatggg gtttgaagtt
    33841 agagtgatag gtaaaggtaa aaataataaa ttagatttag attgtaatcc tgatacagtt
    33901 agagaagaag cagaaagaaa aggagcttca cctcatatga tagcttcttt taaagaagga
    33961 actaaaacaa tggtagaaat ggctttaatg tgtaatgcaa ctggatttgt gcctgatgta
    34021 agaggtggtc atggtattga agctacagtt aatgaagtac ctaagaaata tgcattaaaa
    34081 agcgaaggtg gagtattaga taattatggt gtagttgatt ttgtaaatgg tatagcacct
    34141 ggagttttcg tagtagtagc ccataaatta aaagcagtta atgatgaact aaaatattta
    34201 agtatgggtg atggacctaa ttacatatta tatagaccat atcatttatg tagtttagag
    34261 actcctttat cagcagctat ggcagtctta gaccataagg ctacaatagt acctaaagct
    34321 ggactagtag cagaagttat gactatagct aagaaagatt tgaaaaaagg tgagtttatg
    34381 gatggttatg gagcatatac atgctatggt actatagaaa aatacgatgt agcaaaggct
    34441 atgaatgctg tacctattgg tttgataagt aaaaagacta aagttgtaaa agatattaaa
    34501 aaaggtgaag ttataactta cgatatggta gagatagaaa aagatactac tttatatcat
    34561 ttaagacaat tgcaagaaaa aatatttggg taagatttga tggtaatata aatagatatt
    34621 agtaaaaagg gttgatggtt atttttaaat caaccctttt aaatttttat atattcttgt
    34681 taggatactt tctttaaaag tatattatag gttatttgta gtcgtaaaaa ttattttgaa
    34741 ttagttctgc aattttatat tcacattgaa acaatattat taaaaatttt aatataaaat
    34801 gaattaaaat gaccaattat gtattataat attaactgta atactaatat agttctatta
    34861 attaaagaaa tatcccccat taatatataa taaatatctt taagcgagag aacttagtaa
    34921 aaaatatatc gtattcaata aaaaatctgt ttacagaatg gtatatatag ttatataata
    34981 tactatataa gatgcgaaaa gataaaaagt atagcacaat gaaagaaggt gattattatt
    35041 cagcttaatg aaagacaact aaaaattata gatatagtta aggaaaatga accaataacg
    35101 agtgaaagta ttgcctccag cttgaatgtg actcgtgcta cacttagatc tgatttagct
    35161 atattaacaa tgacaggaat attagacgca agaccaaaag ttggatattt ttattcagga
    35221 gttagtgaaa taaatttaat tggtaaaagt gtaaaagaaa aaacagtaga ggatataatg
    35281 agcatgcctg tactagctaa gaaggatgaa agtatatatg atgtaatagt cactatgttt
    35341 ttatctgatg tagggagtat agttattata gatgagaatg aagagttatg tggagttgtt
    35401 tccagaaaag acttgctaaa agcaactata ggaggttctg atataaataa aatgccaata
    35461 ggtatgataa tgacaagaac tccaaatgta gtcactttaa ctaaaggagc aagtgtttta
    35521 ttagcttcaa gaaaaataat agaacatgaa gtagattcga ttccgattgt tgaatataag
    35581 ggagaagaca aaaatcatat gagagttgtt gggagaatat caaaaaccaa cataactaag
    35641 ctattcttag aaatagttga caattaagga ggtatgctgg tgaaaaacct aattatatat
    35701 gcagtatcag attcagtagg ggagactgca caacaagttg caaaggcatg tatgtctcag
    35761 ttttatgtga atgaaactta tgaaataaaa agatttccat acatgattaa taaaggagta
    35821 ctacttgaaa ctttggagaa tgcaaaagct gaaaacgctt taattgtata tacattagtt
    35881 gatgaagaat tatgcagtat tgttgaaaga tattgcgaaa gagaaggtct tagttgtata
    35941 gatttaatga cagatatact aagagaaata agcaagagaa caggaagaaa acctaaaaga
    36001 gaagctggta taattagaaa attagatgaa tcttacttta agagagttga agctatagaa
    36061 tttgcagtga aatatgatga tggaaaagac cctagaggtg ttcttcaagc agacataatt
    36121 ttagtgggaa tatcaagaac atctaaaact cctttgagta tgtatttagc taataaaaat
    36181 ataaaagtag ctaatgttcc attagttcca gaaataccaa ttcctaaaga agtgtttgag
    36241 atagatacta aaaagataat aggtcttact aattcacctg aaaagctgaa tgagataaga
    36301 acccaaagat taaaagcttt aggattgtcc agtaaagcaa actacgctaa tttagaaaga
    36361 atacttcagg agttagatta ttcagaagag ataatgaaaa gaattggatg cccagtaata
    36421 aacgtttcaa ataaggcaat agaagaaaca gcaggtataa ttttagacat aatgaaagaa
    36481 aatggtctaa agatatacaa agaaatagaa atttaataat gtctattaat aaaaatgata
    36541 taaattaaaa taaaaagatt aatgggggat ttaacaatgg aaactaagta tgtttatagt
    36601 tttggtgaag gaagcaaaga catgaaatct ttactaggag gtaaaggtgc caatttagca
    36661 gaaatgacta agataggctt acctgtccct cctggattca ctataacaac agaagcatgt
    36721 aatgattatt atgttaacaa tgaaagtata agagcagaga taataaaaga aatagaagag
    36781 catttagcta ctttagaaaa agacttaaat aaaacattag gatgcaataa gaatccatta
    36841 ttagtatcag ttcgttctgg tgcagtattc tctatgccag gaatgatgga tacaattctt
    36901 aacctaggtc ttaatgacaa tagtgttgtt ggcttagcag aggctacaca aaatgaaaga
    36961 tttgcatatg atagttacag aagattcata caaatgtttt ctgatgttgc tatggaagtt
    37021 ccaaaatata aatttgaaaa tgtattagac agagtaaaag aagcaaaggg atacacagtt
    37081 gatacagaac ttacaactga tgatttaaaa gaaatagtaa aagaatttaa agcaatttat
    37141 aaaaaagaaa taaaaaatga tttcccacaa gacccaaaag agcaattaat gcttgcaata
    37201 gaggctgtat ttagatcatg gaataatcct cgtgcaatcg tataccgtaa attgaatgat
    37261 atagctcata acttaggaac agcagtaaat attcaatcaa tggtatttgg taatatggga
    37321 gaaactagtg gtacaggtgt tgcatttact agaaatccag ctacaggaga aaataaatta
    37381 tttggtgaat ttttaatgaa tgcacaaggt gaagacgttg ttgcaggtat aagaactcct
    37441 caaaatatat caactcttgc agatgtaatg ccagctgtat tcgatgaatt tgtaaaaatt
    37501 actcatatac ttgaaggtca ttataaagat atgcaagata tagagtttac tatagaaaat
    37561 gaaagattat atatactaca aactagaaat ggtaagagaa cagcagcagc agcaataaat
    37621 gtagcggttg atttagttga agctggaata attgatgaaa aagaagctat catgagaatt
    37681 gagcctaatc aattagacca attattacat ccaaaatttg aagataaggc attaaaagaa
    37741 gccaaggtta tagcaaaagg tttaccagca tcaccaggtg cagcaagtgg taaagtttat
    37801 ttcaatgctg atgatgtagt aaaagcaaat gaaaaaggag aaaaagtagt tttagttaga
    37861 ttagagacat ctccagaaga tatagaagga atggttaaag cagaaggaat ccttacagct
    37921 agaggtggaa tgacatcaca tgcagcagtt gttgctagag gtatgggtaa atgttgtgtt
    37981 gctggatgtg gagaaataaa agttgatgag tttaataaag aaataagagc acttgatgat
    38041 gtagttataa aagaaggtga atacatttct atagatggtt ctacaggaaa tgtgtaccta
    38101 ggagatgtta agaaaacaga agtatcttta actggaaact ttgaaaaatt aatgaactgg
    38161 gtagacaaac acaaatgtat gatggttaga actaatgcag ataatcctag agatgctcgt
    38221 gcagctatag agtttggtgc agaaggtata ggtctatgta gaactgagca tatgttcttt
    38281 gatgaagcta gattgccagc agttagagaa atgatattat ctaatacagt agaacaaaga
    38341 gaaaaagctc ttgaaaaaat attaccaatg caaagagaag attttgtaga gttatttaaa
    38401 gttatggatg gaaaaccagt taatataaga ttactagacc caccactaca tgaatttttg
    38461 cctcatgatg atgagactat agaagaactt tcaaaatcta tgggaataaa agtaagtgat
    38521 attaagaaga gaatagttga tttagatgag tttaacccaa tgcttggaca cagaggttgt
    38581 agacttgcaa taacttatcc tgaaatatgt gtaatgcaat ctaaagctat aatacaaggt
    38641 gctatagaag caatcaaagc aggtgttaaa gtttctccag agataatggt tccattagtt
    38701 ggagaagtaa atgagttaaa aataataaga aaaatgatag tagaaactgt tgacgctata
    38761 ataaaagaag aaggtataga agttccttat actgtaggta ctatgataga aatacctaga
    38821 gcttgtttaa ctgctgatga aatagcacaa gaagcagact tcttcagttt tggtactaat
    38881 gacttaactc aaatggcatt tggatattca agagatgatg ctggtaaatt cttaggtcaa
    38941 tatgtagatg aagaaatatt agaaaaagac ccattccaag tactagacca aaatggtgtt
    39001 ggtaagttag ttaagatggg agctaaatta ggtagagaag ttaaaccaga attaaaacta
    39061 ggaatatgtg gagagcatgg aggagaacca tcttcagtag agttctgtta tagtgtagga
    39121 cttaactatg tatcttgttc accatttaga gtgccaattg ctagacttgc agcagctcaa
    39181 gcatctataa aaaatcctag ataataatag actgcaaaat atgtaactaa ataaaaataa
    39241 aatcccctat tgttcaatgt atcaaattaa gtatttgtaa attgaatata ggggattttt
    39301 attataaaaa tttaacttat acagttgtaa gtgaaacgta tttaattata ttgcatatat
    39361 tattgtagag agaaatttca actatcaatt aaattgagtt tactggaggg gataaagttg
    39421 acaaagtgtc ctaaatgtgg aaaggaagta tcatcacgtc caggtagtat atgcccacga
    39481 tgtggattta aagtagcaga tgagagtcta aaaataagat gtccagaacc tagttgtagg
    39541 gctttggtat ctaggaaact ggattattgt cctaaatgtg gatgcaaact tagaggatat
    39601 aatatttctg aatttgttaa catggtaaga tgtaaaatag atgagacatt tgataaaaaa
    39661 taattaaatt tagagttaag cttttgaggg tgtctcaaaa tgaacttttt agttcagaag
    39721 gcactctttt tgtatggaat caaatatatt tttataattt aaacaataaa a
//
LOCUS       DAEPOZ010000008        38245 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.008, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000008 DAEPOZ010000000
VERSION     DAEPOZ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38245
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.008"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            5..1057
                     /locus_tag="KR440_000356"
     CDS             5..1057
                     /locus_tag="KR440_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBH0697425.1"
                     /translation="MRLNASDIHITVGTNPVARVKGGFVKLSEQILTSEVTMQMAKDI
                     AGESIFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAEIPTFEKLGLP
                     ESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLEDPIEYVHHHKQ
                     SLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAAETGHLVFSTL
                     HTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKGRIAAIELMFA
                     TPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDMALSRCTDQEF
                     MTRMIGGVNYNGYYNR"
     gene            1038..1820
                     /locus_tag="KR440_000357"
     CDS             1038..1820
                     /locus_tag="KR440_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBH0697426.1"
                     /translation="MDIIIVNLYVFIVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGILFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILIFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGLYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILCGKQLLELYFSMVI"
     gene            1922..2572
                     /locus_tag="KR440_000358"
     CDS             1922..2572
                     /locus_tag="KR440_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBH0697427.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLAFFNI
                     AQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            2586..3146
                     /locus_tag="KR440_000359"
     CDS             2586..3146
                     /locus_tag="KR440_000359"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBH0697428.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            3163..6549
                     /gene="mfd"
                     /locus_tag="KR440_000360"
     CDS             3163..6549
                     /gene="mfd"
                     /locus_tag="KR440_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBH0697429.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGAENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            6580..7575
                     /locus_tag="KR440_000361"
     CDS             6580..7575
                     /locus_tag="KR440_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBH0697430.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            7699..8238
                     /gene="spoVT"
                     /locus_tag="KR440_000362"
     CDS             7699..8238
                     /gene="spoVT"
                     /locus_tag="KR440_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HBH0697431.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            8358..9971
                     /locus_tag="KR440_000363"
     CDS             8358..9971
                     /locus_tag="KR440_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBH0697432.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            10078..11526
                     /gene="mazG"
                     /locus_tag="KR440_000364"
     CDS             10078..11526
                     /gene="mazG"
                     /locus_tag="KR440_000364"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBH0697433.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            11615..11893
                     /locus_tag="KR440_000365"
     CDS             11615..11893
                     /locus_tag="KR440_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBH0697434.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            11965..12207
                     /locus_tag="KR440_000366"
     CDS             11965..12207
                     /locus_tag="KR440_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBH0697435.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            12269..12523
                     /locus_tag="KR440_000367"
     CDS             12269..12523
                     /locus_tag="KR440_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HBH0697436.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            12524..13057
                     /locus_tag="KR440_000368"
     CDS             12524..13057
                     /locus_tag="KR440_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697437.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            13063..13347
                     /locus_tag="KR440_000369"
     CDS             13063..13347
                     /locus_tag="KR440_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBH0697438.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            13460..14380
                     /locus_tag="KR440_000370"
     CDS             13460..14380
                     /locus_tag="KR440_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HBH0697439.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIKFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYDEIDKTLNHIKDRGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            14470..16842
                     /gene="spoIIE"
                     /locus_tag="KR440_000371"
     CDS             14470..16842
                     /gene="spoIIE"
                     /locus_tag="KR440_000371"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HBH0697440.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNNEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            17193..19085
                     /gene="pepF"
                     /locus_tag="KR440_000372"
     CDS             17193..19085
                     /gene="pepF"
                     /locus_tag="KR440_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBH0697441.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAIAVMNNN
                     DTEDDAEKKQEVDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMSDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESVDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            19199..20164
                     /gene="galU"
                     /locus_tag="KR440_000373"
     CDS             19199..20164
                     /gene="galU"
                     /locus_tag="KR440_000373"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBH0697442.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
     gene            20664..22268
                     /gene="rho"
                     /locus_tag="KR440_000374"
     CDS             20664..22268
                     /gene="rho"
                     /locus_tag="KR440_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBH0697443.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQENRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            22421..22621
                     /gene="rpmE"
                     /locus_tag="KR440_000375"
     CDS             22421..22621
                     /gene="rpmE"
                     /locus_tag="KR440_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBH0697444.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            22766..23659
                     /locus_tag="KR440_000376"
     CDS             22766..23659
                     /locus_tag="KR440_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HBH0697445.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            23677..24525
                     /gene="prmC"
                     /locus_tag="KR440_000377"
     CDS             23677..24525
                     /gene="prmC"
                     /locus_tag="KR440_000377"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBH0697446.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            24569..25633
                     /gene="prfA"
                     /locus_tag="KR440_000378"
     CDS             24569..25633
                     /gene="prfA"
                     /locus_tag="KR440_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBH0697447.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            25762..26442
                     /locus_tag="KR440_000379"
     CDS             25762..26442
                     /locus_tag="KR440_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBH0697448.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            26471..27511
                     /locus_tag="KR440_000380"
     CDS             26471..27511
                     /locus_tag="KR440_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBH0697449.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            27528..27977
                     /locus_tag="KR440_000381"
     CDS             27528..27977
                     /locus_tag="KR440_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HBH0697450.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            27994..28446
                     /gene="rpiB"
                     /locus_tag="KR440_000382"
     CDS             27994..28446
                     /gene="rpiB"
                     /locus_tag="KR440_000382"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBH0697451.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            28482..29111
                     /gene="upp"
                     /locus_tag="KR440_000383"
     CDS             28482..29111
                     /gene="upp"
                     /locus_tag="KR440_000383"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBH0697452.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            29411..30226
                     /locus_tag="KR440_000384"
     CDS             29411..30226
                     /locus_tag="KR440_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HBH0697453.1"
                     /translation="MKNCIAVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            30452..30889
                     /locus_tag="KR440_000385"
     CDS             30452..30889
                     /locus_tag="KR440_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HBH0697454.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            31074..31301
                     /locus_tag="KR440_000386"
     CDS             31074..31301
                     /locus_tag="KR440_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HBH0697455.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            31306..31692
                     /locus_tag="KR440_000387"
     CDS             31306..31692
                     /locus_tag="KR440_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HBH0697456.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAIRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            31695..32399
                     /gene="atpB"
                     /locus_tag="KR440_000388"
     CDS             31695..32399
                     /gene="atpB"
                     /locus_tag="KR440_000388"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBH0697457.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            32468..32728
                     /gene="atpE"
                     /locus_tag="KR440_000389"
     CDS             32468..32728
                     /gene="atpE"
                     /locus_tag="KR440_000389"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBH0697458.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            32851..33348
                     /gene="atpF"
                     /locus_tag="KR440_000390"
     CDS             32851..33348
                     /gene="atpF"
                     /locus_tag="KR440_000390"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBH0697459.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            33345..33890
                     /locus_tag="KR440_000391"
     CDS             33345..33890
                     /locus_tag="KR440_000391"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBH0697460.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            33907..35409
                     /locus_tag="KR440_000392"
     CDS             33907..35409
                     /locus_tag="KR440_000392"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBH0697461.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            35437..36315
                     /gene="atpG"
                     /locus_tag="KR440_000393"
     CDS             35437..36315
                     /gene="atpG"
                     /locus_tag="KR440_000393"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HBH0697462.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            36328..37722
                     /gene="atpD"
                     /locus_tag="KR440_000394"
     CDS             36328..37722
                     /gene="atpD"
                     /locus_tag="KR440_000394"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBH0697463.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            37725..37985
                     /gene="atpC"
                     /locus_tag="KR440_000395"
     CDS             37725..37985
                     /gene="atpC"
                     /locus_tag="KR440_000395"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HBH0697464.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
BASE COUNT        15333 a         4151 c         7209 g        11552 t
ORIGIN      
        1 aggtataaga ctaaacgcat cagatataca cataacagtt ggaacaaatc ctgtagctag
       61 agtaaaaggt ggatttgtaa aactaagtga acaaatattg acatcagaag taacaatgca
      121 aatggcaaaa gacatagcgg gggaatctat ttttaaagta atagaagaac atggagaagc
      181 tgatttttca gcttctctaa aaacaggaga aagatttagg gtaaatgcat ataggcaaaa
      241 aggaaattac gcaatagcaa taaggactat aacagcagaa attccaacat ttgaaaaatt
      301 aggccttcca gagagtataa aatcttttac tgaaaagcat aaaggattag ttttagttac
      361 aggtccaaca ggaagtggta aaagtacaac acttgcaagt atgataaata taataaatga
      421 aaaacaacaa aaacatataa ttacacttga agacccgata gaatatgtac atcatcataa
      481 acaaagttta gtaaatcaaa gagaagtagg aactgataca gaaagttttc attcagccct
      541 tagagctatt ttgcgtcagg acccagatgt aatactaata ggagagatga gagaccctga
      601 aactgtttca atagcattaa cagcagcaga aacaggtcat ttagtatttt caacactaca
      661 tacagtaggg gcagctaaga caattgatag aatagtagac atgtttcaac catcccaaca
      721 gcaacaaata aaaacacagt tatctacagt ttgtgaaggt gtggtatcac agcagttact
      781 tcctacagca gatgggaaag gcagaatagc agcaattgaa ttgatgtttg ctactccagc
      841 aataaaaaac ttgataaggg aaggcaaaac atatcaaatt ccaaacatga tacaaactgg
      901 agttaaagta ggtatgaaaa ctatggacca agatttaatg gaactttata aaaatggaaa
      961 gattacaaaa gatatggcac ttagtaggtg tactgaccaa gagtttatga caagaatgat
     1021 aggtggagta aactacaatg gatattataa tcgttaatct atatgtattt atagtgggta
     1081 tagtgtttgg aagtttcttt aacgtctgta tttttaggat tccagaaggt atatctattt
     1141 ctagacctcc ttcccattgt atgagttgta atactagact aaagccaata gacttaatac
     1201 cagtgctaag ctatttgatg agtggcagaa agtgtagata ttgttatgag aaaatttcta
     1261 gtagatatgc tatagtggaa ttaattacag gaattttatt tttaacagta tttatgttat
     1321 atggaatatc tacatcaact atttattatt tggttcttgt gtctttactg attataatta
     1381 catttataga tattgaccac tttataatac cagataaaat attgattttt ggagctatat
     1441 tttctatgat ttttaacctt ttatttaaag aaattccagt taaggattca attctaggtg
     1501 tttttatttg tggaggaagt gtctggatta tagttctttt gattgaattt gtaataaaaa
     1561 aagagtgtat gggtggagga gatatcaaac tatttgcaat gctaggcctt tacatgggtg
     1621 ttaaaaatgg tcttttgaca gccttactaa gcgtttatgt aggagcagtc tatggaatct
     1681 gtgtaataat tataagtaga ataaaaggaa aggaatataa ttctgttata ccatatggac
     1741 cttttatatc tattggagca ttaataacaa tcttatgtgg aaagcaatta cttgaacttt
     1801 atttttctat ggttatatag atgatagtca agttaaataa agttttaatt taaaaaaatg
     1861 aattaaagta tttaataagg acaatattat tattaaccct attactaaag tgctacaaac
     1921 tatggagtat ataataataa tttccttatt ttttgcacat ttgataaata aatctctatg
     1981 tgttttttgt gatagagaaa agaaaaattt atataatacg ttttttattt acataataac
     2041 tgtagtttta tctgtattaa tatatctaaa atttgggatt agtgtagaat gtgtacaata
     2101 tatggtttta attccatttt taataattat atccattgta gattactaca caatatatgt
     2161 atatgatata acaatagtta gtggtataat agttcaaggt ataatttttt tggccttttt
     2221 caatatagct caatttgatt atataaatca tatatttgga ctattgatag gctttattat
     2281 accttatatt attgctaaac taacaaaagg attaggctat ggagatgtag gtctctatag
     2341 cttatgttgc tttgcattgg gaaataatta tgggttttat gtgatagttt tatcatttat
     2401 tttagcgttt ttgtttttta ttattttata cataactaaa atatttaaag atgtagatat
     2461 aagaaagcaa attcctttta caccttttat ttccttgtca acaattttaa taatatttac
     2521 agagtataat atatttaatt tttattataa tttgttatac aaatttatat aacaggaggt
     2581 aagaaatgta cgtagtagta gggttaggta atccaggaaa gaagtatgag aagactagac
     2641 ataacgttgg atttgatgtt atagatattc ttgctaaaga atacaatatt agtgttacta
     2701 aaataaagca taaagctttg attggtgaag ggagagtagg tactgaaaaa gtattattag
     2761 ttaaaccaca aacttatatg aatttaagtg gagaaacatt aatagatata tataaatatt
     2821 ataaggttga tttaagtaat atagtagtag tatatgatga tatagattta gaagttggaa
     2881 agatacgtat aagaaaaaaa ggaagtggag gaactcataa tggcatgaaa tctataacta
     2941 agtgcctagg ttcaaatgac tttccaagag tgagagtagg tgtatctaaa ccagaagctg
     3001 gtcaagactt agcagacttt gtactttcta gatttagaaa agaagaatct gataacataa
     3061 atgaagcatt agaaaaagcg gcagatgcta ttgactcaat aataagagaa aatatagata
     3121 tgtctatgaa caaatataat ggatagtgag agggagattt aaatgaatga tgttttttta
     3181 tatcctttgc aaaattcaaa agagtataaa gatataataa actgtataaa aaatacaaaa
     3241 ggctcattat tggttaatgg acttttacca gtacaaaaac ctcatatatc gtattctata
     3301 tttaatgatt tatcacgaca aatgattttt ataacgagta gtgatttaga agcaaagaaa
     3361 gtatacgaag atttaagttt ttatatggaa gataaagttg aatatttagg ttttcaagat
     3421 atttattttt atcacttgga tgcaaaggac agaaacgaag aagcaaagaa attaaaagta
     3481 ttgttaaaac tagcaaataa agaaaaaatc attttagtaa cttctattga ggcagtttta
     3541 agaaaataca taccaaaaca agttttgctc gatagtgtat ctcattataa ggtgggagat
     3601 agcttagact tagaaaagtt aactgagaaa ttagtttcat tgggatatga aagagtttca
     3661 aaaatagagg ggtttggtca atttagtata agaggtggaa ttattgatgt attttcactt
     3721 gaatatacaa atccaattcg tatggaatta tttgatgatg agatagattc catacgaact
     3781 tttgacgtat attcacaaaa atcaattgat aaattgcaac aattctctat aactccatct
     3841 agagaattta tttatcccga aaaaactaca gatgcattag ttaaattaaa aaaagaaaca
     3901 actaaaaata ctgatgaagg tacatttcaa aatatagatt atatatcaag taagacttat
     3961 tttgaaggtg cagagaacta tatagattat atttatccag aagaaaacaa aagtatattt
     4021 acttatttag cagatgatgc aatagtattt attaatgata taacgagact taaagaaaga
     4081 tgcgaaaatt atataaatga gtttagagaa aattataaat taaatcttga aagaggtctg
     4141 gctattaaaa accaaggtaa attgttatat cactatacag atttagagta cttagtaaaa
     4201 gacaagtctg tggtattaaa ctcgctattg ccaaagtcta taaataactt ttctataaaa
     4261 tctataataa attttgaaag tagagaagtt cctacattta atggaaaggt agatttgctt
     4321 gtagaagaat taaatcggct gaaatataat ggatacaaaa taattttagc aacaaatacc
     4381 ttagaaagag caaataaatt aggaaaagat ttattggaca aaggcttaga aacgaccatt
     4441 tctaaagata gagatattga aattaaatct tcacaagtta taattgtacc tgcacacata
     4501 aacagtggtt ttcagtataa atctataaag tttgttgtta taacagataa cgaaatgata
     4561 ggtgtttata aaagagcgtc taagactagc aataaaaaag ttaaaaaagg aaagaaaata
     4621 gaatcctttt tagatttaag tgttggagac tatgtagttc atgaaaatag tggtgtaggt
     4681 agatacacag ggattgaaca aattacagtc aatgctatta aaaaagatta tatgaaaata
     4741 gtatatcaag gtggagataa tctatatgtg ccaatagacc agatggacaa agtacaaaaa
     4801 tatattggtg cagaagtaga aaaagttaag ttaaataagc ttggaacaaa tgagtggacg
     4861 aaagcaaaag caaaagttaa aaaagaaatt gaagatatga caaaagactt gattgagctt
     4921 tatgcaaaac gtgaaaaaat acaagggtat aagttttcaa aagatacacc ttggcaggca
     4981 gagtttgaat cgttgtttcc atatcaagaa acagaagacc aattaaaggc tatagaagaa
     5041 actaaaaaag atatggaatc aagtaaggta atggataggc tagtatgtgg agatgttggt
     5101 tatggaaaaa cagaggtagc tattagaagt atttttaaag cttgtatgga ccaaaaacaa
     5161 gttgctgtat tggttccaac aacaatattg gcccaacaac attataatac attcaaagag
     5221 aggtttgaaa attatccttt gagagtagaa gttttaagta ggtttaaaac accaaaacaa
     5281 caaaaacaga ttattgaaga tgctaaaaaa ggattagtag atattttaat tggaacacat
     5341 agaataatat ccaaagatat aaatttacca aacttaggtc ttgtagtcat tgatgaagaa
     5401 caaagatttg gagtaaaaca taaagaagct ttaaagaaaa ttaaatctac agtagatgta
     5461 cttacacttt cagcaactcc tataccaaga acattacata tgtctttaag tggaataaga
     5521 gacatgagtg ttatagaaga acctccacaa gaaagacatc ctgtaataac atatgttaca
     5581 gagagtaagg aaagcgtaat tcaagatgag atagagagag aattaagtag aggtggacag
     5641 gtattttttg tatacaatag agttgaacac attgaagaaa tggcaagtat gattcaaaaa
     5701 ctcgtaccag atgctagagt ggctgttgca catggaagaa tgacttctaa atcattagaa
     5761 aatataatcc taggattttt aaataaagat tatgatgtac tagtctgtac tacaataatt
     5821 gaaactggta tggacatatc aaatgcaaat acaatgatta tatatgatgc agataaaatg
     5881 ggattggctc aactttacca attgagaggt agggttggaa gaagttcaag acaaggatat
     5941 gcatatcttt tgtatgaaaa agataaaact ttaagtgaga tagcggagaa gagattaaaa
     6001 gctatcagag aatttacaga gtttggttct ggttttaaaa tagcaatgag agacctcgaa
     6061 ataagaggag caggaaatat tttaggttct caacaacatg gtcatatggc agttataggt
     6121 tatgatttgt acgttaaaat gcttaatgat gcaattaaaa aagtcaaagg tgaacctatt
     6181 gtggaggaaa ttgatgttga aatagattta agtgtaaatg catacattcc agataattat
     6241 atcaaagatg agttgattaa aattgaaatg tataagaaaa tagcttctat tgaaaacaaa
     6301 gaagacatgt tggatataca agaagaactt gaggatagat tctctgatat acctaaacca
     6361 gtccagacac ttcttacaat agcttatata aaatcgttat gtaagatatt aaaaatagaa
     6421 aagataagac aattaaaaga tgaaatatta ttagttccaa taacaaagta tagaactaaa
     6481 cagaaaatag gttataatat agttacagaa ttagaagaat tattagaaaa gatgtgcaag
     6541 gtaaaataac ttgtaaattt aagaagggat gttttaaatt tgaaaaaagt aataacattg
     6601 gtaatagcta tgattttggt agtatctgtg actgcttgca gttctagtaa aggagaaact
     6661 gtggcaacag ttgaaggtac aaagatatca tcagatgaat ttaaaaaaac tatagctctt
     6721 tataaagatt ctatggaaca aacttatgga aaagatatat gggataagga agttgaaaag
     6781 ggtgtaaaat acaaggacaa gtttaaagat ttaattcttg accaacttat aactacagaa
     6841 gtaatatatt ctcaagctaa aaaagataac ttacttccta aaaaagagga tgttgaaaaa
     6901 agttttaaag aattaaaaga cgctatgggt aaagatgaaa aatataaaga acaacttaag
     6961 aaattaggta tagatgatga gtttttaaga gaccaacaag agaaagattt agcaatgcaa
     7021 aactatcaaa gtaactttgc taaaaagact aaaatatcag atgaagaaat gaaaaaatac
     7081 tatgatacac ataaagatga atttaaaaaa gatgaggtag aagcttctca tattttattg
     7141 aaaacagtag atgataataa caagccactt tcagataagg aaaaagctga agctaagaaa
     7201 aaggctgaag aggctcttaa agaagttaaa tcaggtgagg attttgcaaa agtagcaaaa
     7261 aaatattcac aagatacttc agcaagtgat ggtggtaaat taggattctt ttcaaggggg
     7321 caaatggttg ctgaatttga agatgctgct ttttctatga aaaagggtga agtatctgat
     7381 ttagttgaaa ctcaatatgg ataccacata attaaagtga cagacagaat aaatgagcaa
     7441 acatcatttg aagatgctaa agaaactata aaagaccaac tgttgaaaaa taaatatcaa
     7501 gaacaaatag aaaaattaac taaagaagct aaggtagaaa aagatgaaaa agtaataaat
     7561 aaaatcacaa tctaagactt aaaaaaagag taatatgctt aaaaaagcta tatctggata
     7621 agatatagct ttttttgtat atttttctta acaatggtaa aaataaactt aaagtaattt
     7681 atggagggat taaaaaatat gaaagcaaca ggtatagtta gaagaataga tgatcttgga
     7741 agggtagtta tcccaaaaga gataagaaag acattaagaa ttagagaagg agacccttta
     7801 gagatattca cagcaaaaga tggagaagtc atacttaaaa aatactctcc aataggtgaa
     7861 ttaaatgaat tttcgcaaga atatgctgaa acattagctg aagtactagg atatggagtg
     7921 ttgataacag acttagattc tataatagct gtgtctaagc ttcctaaaaa agattataaa
     7981 gaaaaaagta taagtgatga gttagaagca ataataagtg aaagaacagt aaaatattca
     8041 aaagataaaa acattttacc attattcaag gaagaccaaa aagaatatac tagtcaagtt
     8101 atcatgccta taattagtgc atctggtgac tgtataggtt caattgtagt tgtttcaaag
     8161 gataaagata caattgcaga aagtgaagaa aaatctttaa aaattgctgc taacttttta
     8221 ggaaaacaag ttcaataatt ttatcataaa gaccttttac atgaaggtct tttgaagttt
     8281 ttgtgtaaaa tattgaatat agcattaaaa atatggtata ttaaagtttg ttatataaac
     8341 agtatggagg ttatgttatg aacaataaca aaaataaaga ctcctttcta aaaggagccc
     8401 ttatacttgg gctagcaggt gtaatcgtaa aaataatggg tggatttttt aggattcccc
     8461 ttggaaacat gattggttca gaaggaatgg gatactatca ggctgcttat cctgtgtata
     8521 ctctattttt aacattagct actgctggat ttccaacagc tgtggcaaaa ttggtttcgg
     8581 aaaaagttgc aattggaaat tttaaaggtg caaataagat atttaaggta tcacatacag
     8641 ttttattcat taccggtata atatcatttt gtatattatt ttttggagcc gactacatag
     8701 taacaaacgt aatgaaaaat ccaggtgcgt tatactctat gaaagccatt gctccagcat
     8761 tactatttgt tcctgcaatg tctgcatata gaggatattt tcaagggaga caagatatga
     8821 ctaagattgc tgtgtctcaa gttgcagagc aattttttag agttgtatta ggtcttactt
     8881 tggcctactt gcttatgaag agtctaggtc agacttttgg tgcagcaggt gctatatcag
     8941 gggctacaat aggttcaata gcatctatgc tttatttggt gtttgcttat atgctaggga
     9001 gaaaagagcg tagagcagag atagatgcaa gccaaagatt taaagatgaa agagtgtctt
     9061 atatttttaa aaagctttta actgttgcca ttcctataac aataggagca tcagtaatgc
     9121 cacttgtaaa tatgatagat aatgttattg taataagaag acttatggaa gcaggattta
     9181 catacaaagt ggcaaattca atgtttggtc aattaacagg tatggcaatg gccacaataa
     9241 atttaccagc agtcataaca actgcaatga gtatgagctt agttccagca atttctaaag
     9301 catatgcatt aggaaataag tcaaaagcta gaaaagatac taagagtgca gttaaggtaa
     9361 ctttacttat agtattgcct tgtgcgtttg gtatggcttc tttagcaata ccaataatgg
     9421 gtcttttatt tccacatgaa ccatcttctg taggaactat tttgtttact cttactccat
     9481 gtgtattatt tttaggatta atacagacat tgactggtat aattcaggga atgggaaaac
     9541 caatagtacc tgtaattgct ttatgtgttg gtatgctatg taaaattgta ataagttata
     9601 ctttaacagg tataccagat attaatgtac tgggttcagc atttggtaca gttacagcat
     9661 actttgtggc agccatgatt aacttactat atgttaaaaa acacatgaat gtaaactttt
     9721 ctaaaaaaga atttataata aagccattta taacagttat gacaatgttt ataatggtta
     9781 agttgagtta tggtgcttta gttggattct taggaaacag catatctaca ataattgcaa
     9841 tatgtgttgg tggaattgta tatgtagtag taatacttgg aataggtgga attaaaaaag
     9901 aagagattct aactatgcca aaaggtgata aattatataa attgttaaaa aaagttaaac
     9961 ttataagata atataggaat atatatgcaa ataaaggaaa tactatacaa atagtaaatc
    10021 tccttatttg tataataata taaaaataaa gtgaaatttg ctaataggag tgatgttatg
    10081 ggtaagataa gtattgtagg tcttggacca ggagactatt ctcttataag ccaaggtgtg
    10141 ttagaatctt taataaatag ccctaaagtt ttttttagaa cagagaaaca tccaataatt
    10201 gataaactta aagaaactat acaatacact tctttagact acttttatga aaaagaggaa
    10261 aattttgata atgtttattt gaaaatatct gaatttatca tagaagaatc agcaaagggt
    10321 gacctagtgt atgctgttcc tggtcatcca agagttgcag agaagactgt aagtataata
    10381 gagagattag ccaatgaaaa gcacatagca gtagaaatat tgccttctat gagctttgta
    10441 gacgctatgt ttaactattt ggctatagac ccatctgagg gatttaaact ggtagatgct
    10501 tttgaaatag aaaattcata tatagacttg gatacaagca tgataataac tcaggtatat
    10561 gataaattta tagcatctaa tgtaaagtta aaacttatgg aacattatga ctataatcaa
    10621 gaagtatgta tagtaaatgg agcaggtgtc aaagatttag aaagtaaaaa gtttataaag
    10681 ctttgtgaat tagacagaaa tgaaaacttt tttgactatt taactagctt atatataccc
    10741 aaaagttcta aaaagatgta taatacagta catgacttag aaataatagt taatacactt
    10801 agaagtccat ctggatgtga atgggataaa aaacaaacac atcaatcatt aaaaaattct
    10861 gttatagagg aagcatatga actttgtaat gcaattgata ataatgatat agatgaaatg
    10921 gtagaagagt taggtgatgt tcttttacag gttatattcc attgtcagat aggaaatgag
    10981 gagggctact ttgatttaaa agaagtagta aatggcatat gtaaaaaatt aattcataga
    11041 catcctcata tatttagtgg tgcaaaatta gatatgtcta agtttgagaa aacttgggaa
    11101 gaattaaaga aagaagagaa aggcgaaagt actataacag atggccttag aaggatacca
    11161 aaacatttac cagctcttat gaaggctagt aaagttcaac ataaagcagc acttgtcggc
    11221 tttgattggg acaatgtaga agatgtattt aaaaaaattg aagaagaata tcaggaatta
    11281 cttgacgaac acagggtcgg gaatataaaa tacataaagg aagagctagg cgatttatta
    11341 ttttcgatag taaatctagc tagattttta gacatagact caggagaagc cttgaactgt
    11401 actacagaaa aattcataaa tagatttgac tttatagaaa aaagtgcaat gaacttgaat
    11461 aaaaaattag aagacatgac acttgaagag atggatgagt tttggaatca ggctaaaaaa
    11521 caataaaatt gtggaaggaa atttgtcaaa aatctagaac ttttatcaca aagatagaag
    11581 tctatattat aaacattttt aggaggtaaa tatcgtgaat aaagctgaat tagtatcaaa
    11641 gatggcagaa aaaagtggat taacaaagaa ggaagcagaa gctgcgttaa acgcatttat
    11701 gagttctgtt caagatgcac tagtaaataa tgaaaaagtt caattagttg gatttggaac
    11761 atttgagaca agagaaagag ctgctagaca aggaagaaat ccaagagatc cagagcaagt
    11821 tatagatata ccagcttcta aagcaccagt tttcaaagct ggaaaaggat taaaggatat
    11881 aataaatgga taattagaaa tcttgtgaaa aagtgtggat aaatttgcca cactttttct
    11941 atagaaccaa ggaaggtgat aattatgaga ttagataaat ttttaaaagt atctaggatt
    12001 ataaaaagaa gaactgttgc taaagatgca tgtgataaag ggattgtcac tataaatgga
    12061 aaagttgcta aatcttcttc agaggtgaat attggagata cattagaaat acaatttgga
    12121 gagaagaaga tgaagtttaa gataaatgaa gtaaaagagc atgtattaaa aaatgatgca
    12181 aaagaaatgt acgaaatttt agaataaaat taatagtcct ctagtatatt aatagtagta
    12241 ttattgaaat atttactggg gggatattat ggaacagaac ataactttga aagatagatc
    12301 aaagctggtt atttcgggag tcgagcacat atactctttt aatgacaaaa gagtagagct
    12361 caaaacttct gtaggcgaga tggtaattga aggcgaaaac ctagatatga gcaagctaag
    12421 tatagacgaa aatataatta gcatagatgg aactataaat tctatggtat atgcaaagcc
    12481 taaaaagcca caagagagtt tcttgaaaaa ggtatttaga tagatgatac cttttactca
    12541 agatgtaagc attttttatg ctactattta tggtggaata ctcatagggg tgttgtttga
    12601 tttttataga gggctaagag ggaattttaa attcatcaat tattttgcta taatctttga
    12661 tgtattgttt tggtttctag ctacggttat aatattcgta acaataaacc ttacagaatt
    12721 ttttgattta agatattatc attttgtggc tttgtttatt ggttttattt tatactataa
    12781 caccataagt aaaattgttt tatcaattat caataaaata attcgttttg tgagaaattc
    12841 ttttaaaaaa gttacacatt acatagtcag ttttttaaat aatttgtatt acgttataat
    12901 ctattcacta cacctactat ttgatattat attttatata ccaaacatat ttatagcaac
    12961 aagaaaaagt ataaaaagaa aatcaaataa aaaattaaag aacaagaaaa aatcaaaacc
    13021 taagaagaaa aaaaataaaa ctaaaaaaag ggtgtaggaa aaatgaactt aagaaaaaaa
    13081 ttttcaggac agattattgt tatcagttta tttttaggaa tatcaatttt tagtatgatg
    13141 acaggatttg tatttgagta tacaaaagct aaggaatata aaaaagaaat agcaagtcta
    13201 aataaacaat taaaaaagac tgaaatacaa ataaacagct taaagaaaga tgaaaaatca
    13261 tatgaaggag acttagagga tatagcaaga aaaagattga atatggtaaa accaaatgaa
    13321 acagtttatg tagacataaa tcgttgatta aaacttgcta ataagtttac agatgtattg
    13381 attttaaaat ttaagattta ataaaaaagt cattcagtta aggatggctt tttgttttta
    13441 ttgaggaggg aatttcataa tgaaagtagg agctatagat attgggacaa actcaatgag
    13501 attactgata gcagattata gagatgggaa ttttataaac agaaaaaaat ttgttaatac
    13561 aactagaatc ggttatggtg tggatagtaa aggatatata aaagaagatg ctatcaaaag
    13621 aaatataaaa gcattaaaag aattttctga taagtgtaat gatgaaaagt gtgaaaaaat
    13681 ttattgtatg gggacatctg ctcttagaga cagtaaaaat ggagattact ttgttaaatt
    13741 agctaaagaa gaaactggaa tagatgttga tatagttacg ggaatagaag aatcaaattt
    13801 gggctttaag ggtgttttag aaggaataca aggtgatgat actattattg ttatagatat
    13861 tggaggaggt tcaacagaat ttattgtagg tgattcagaa ggaattaaat ttaataaaag
    13921 tgagaatgta ggtgctctta ggatgacaga gaggtttttg aagcacgata ttattgaaga
    13981 tgaagagttt gaagctatga gttcatttat atatgatgag attgacaaaa cgttaaatca
    14041 tataaaagac agaggattaa aaactcttat aggtatagga ggtacgatta catcattatc
    14101 agcaatgaat caagaattag agatttactc tatggaaaaa atacataata gtaaaataca
    14161 caaaaaagaa atagaagaaa ttctacaaaa attaataaag atgacattaa gtgataaaaa
    14221 gaacttaaaa gggctacaag aaaaacgtgc ggatattata actgctggtg taaaaatatt
    14281 gaatataata atggaaaaat tagaaataga agaaataacc gtaagtgaat acgataattt
    14341 ggaaggctta atatgtcaaa agtcaaaaaa aatgtattaa tattttttga tttttaaaac
    14401 cgattatgac aaaaaaaatt actcctcttt gttacaattc ttaatataaa caaaagagga
    14461 ggttttttta tgcaaagaag tgtagcagtt gtgaagaata agactgtaaa accaaatgtc
    14521 ataaaattaa taagcggata tattgatttt actacggcgg cattcgctgt gatgggattt
    14581 ttacttagta ggtctattat agtagactct gtagctcctt taggaattgc cttttttata
    14641 tgtgcatcaa agattgataa gtacaagata cctgtattca tatcaacatt attaggaata
    14701 ttgttatcat ctaatgattt tggaaatgta gcaaaatatg ctgtgtgcct tacaatattt
    14761 atggctatga ataaaaaatt aaaattatca gattcaattg ctaaggttgc atttataggt
    14821 gcaataatac tacttccaat ctccatggga caagctttct tttcaaatag gtatgtatat
    14881 ggtcttctta tagcaggagt agaagcaata ataatgttta tatctatata tgtgttttct
    14941 tatgggatag gtttaatggc caatgtcaac aataggatgt ctgtacggac agaagaagct
    15001 atatcaataa gtttactgct aacctttagc ataatgggaa taggagatat atctctgttt
    15061 ggaatatcaa taaggaccat tttatcgacc gtattgatat tgctgacctc aatagtaggt
    15121 ggagctacca tgggagcatc tagtggtgta attattggtg tagcatccat tttaaataat
    15181 atgacatcag ctgtttatat ggggatatat tctttttcag gattaataag tggagcattt
    15241 aataaaataa ataaatattt ctgtatacta ggatatattc taagttggac aataatatac
    15301 ctatatactt ctggtattac ttctaatatg atgcaattaa gagatatact attaggttgt
    15361 ttgatagtac tagtattgcc agagagatta tttaataaga ttgaaaaatt aataaaaagt
    15421 aatgtggctt caaatgaaat cgtatacgat tatattatga gaagcaaaaa cctaactaat
    15481 agcagattga acagtattta taaaacttat gatgaccttg cagatacatt tgataagata
    15541 agagaaaaag acaaagtgct agaccaaaga gatatagcta atgtaataga tatgattcat
    15601 aatgatgaat gtaaaagttg tagtatgaga agaatgtgct gggagtcaag atttaatcat
    15661 acatacacta tgatatataa cattcttgaa aaaattgagg agaaagggga gttaagtcta
    15721 aacgatatac ctaaaaattt tagaaaggaa tgtatgaaac cagagtcgat agtgaaaata
    15781 tcaaatcact attacaaaat gtttgtatta gattatgatt ggagtgtgaa gttttcagaa
    15841 agtagaaagt taatagcaaa tcagataaga agtatttcaa aatccattaa atccttatct
    15901 caagatttag aaggtgacat aatgctagat atagaaaagg aaaaaaatat atatgaacag
    15961 ttagaaagat atgatataac agttgataaa gtaagttact taacaaaaag taacaatgag
    16021 tttgaaatat caatagaaaa gaaaacttgt catgatggat gtatgtgtga agataaaatt
    16081 gtaaatataa tttcagacct agtaggtgaa aatatgtctg ttagaaaaat aggatgtcac
    16141 tgtttaggag gtaaatgcaa ggcaacattc gttaaatctc aaaaatataa ggctgttaca
    16201 gaagtttcag caatgtcaag ggatgggcat atcctttgtg gagacaatta cacatacatg
    16261 gagataaatg atggtaaata catgatggct ataagtgatg gtatgggaaa gggtaaaaaa
    16321 gcatatgaag aatcttctgc cactattgat atactggaaa aaatgataga tgcaaaaatt
    16381 aaagatgaga tagtcattga tactataaat aatatgttgt tgttaaaatc ttcagaagag
    16441 atgttttcaa ctctggattt gggaatttta gatttaaaac gaggttgttt agaaactata
    16501 aagatgggag cgtgttctac ttatataaaa agagaggatg gagaagttga cttaatatca
    16561 tcatcatcat tacctgtggg tatattatca gatgtaaaaa ttgatagaaa aaatgtaaaa
    16621 gttaaggaag gagactatgt tataatggtt tctgatggaa ttgtagatgc aggaagaaat
    16681 aacaatttag gagacaattg gttaatatac ttcttaaaga atatagagac tacaaatcca
    16741 aaggaaattt caaatttaat attggataga gcattagagt tacaagcgtt acaaatcgaa
    16801 gatgacatga ctgttttagt tacgaaaata tgtacaaaat aaggtttaca agattatgtg
    16861 tataagatgt ggataacttg tgtataatat gttaatagac ttttgaaaaa agtatagtta
    16921 ttaacaaaaa taacatataa atcaacaaaa tttgttgata agtatggcag aaatgttaat
    16981 aaaatgtaaa aaaagaaaaa acaaaagcta ttatcaaaag agattcatgt tgaaataact
    17041 aaatttctaa gtaaagaaaa cctgtgaata tgttgattat tatgtggata actattaatt
    17101 cgatatttat ttatatagta tgaataattg aataatatat ctaaaaaaag ccatcctatt
    17161 attaagtaaa tttattgaaa ggatggcttt ttatgaatag aaaattgtgc aaagcaataa
    17221 tacctttatt tatttctatt ttttgcatgt taataataag tataaaagct gaccaaggat
    17281 atcaaaagaa taatgacaga gcaattgctg tcatgaataa taatgatact gaagatgatg
    17341 ctgaaaaaaa acaagaagta gactatgaaa aaacatgttg gaatttgcaa tctttgttca
    17401 aaagtgatga ccaatggaaa aaagaactta agagttttga taaagatact aaagagttga
    17461 aaaattacat aggtaaagta acaaaatcgc caacacattt gtcttttgcc ttaaacatta
    17521 aggaaaaact tgatataaga ctaaataaac tttctgccta tgtaaaatta aaacaagata
    17581 ctaataaaaa ttcatataag tacttagata tgagtgatag tatgagtaaa tcatatggga
    17641 attatatggg gatttgttca gatttagaat tagaaatact aaaattatca aacaaagatt
    17701 acaagaaaat tatttcaaat aaaaatataa ataggaaata tggtgcatat ttaagagata
    17761 taagaaggaa taaagtacat tatttggatg ataaatcaga agatattcta agcaaagtat
    17821 cttcaataag tagtcttcca agccaagtat atgaattatt tagaaatatg gatagaaaaa
    17881 ctaatttgac accagcgcaa tatgcaagtg aattagagtc agtagataga gaaaatagaa
    17941 aaaaagctta tcaagatgaa cttataactt acaatgataa tataaataca attgcaggtt
    18001 taattactgg tcaagttaaa aagaatattt tttattctca ggaaagaggt tatgagtctt
    18061 ctttagaaat gtacttggaa tctgataatg tagatgaaaa agtatacaat aatttgataa
    18121 ggactgtaaa tagtaatatg gacagtctac acaaatatat agatttaaga aaaaaagtat
    18181 taaaattaga taaggttcat tcatatgata tgtttgtgcc tatggttaag gctgttgata
    18241 ataatattaa ttatgaaaag gcacaaagta tagtatattc agcgttgtca cctttaggaa
    18301 aagaatacaa tgatgtagta tataaagctt tcaatgaaaa atggatagat gtttattcac
    18361 atgaaaataa agtaagtggt ggatactgct tatctgttta tgaaaatcat ccatatatac
    18421 ttttaaatta taataattca ttaggttcag tttcgacttt atcacatgag cttggacatg
    18481 ctgtatatga atacttaagt tctaagaatc agaattattt taattcaaat ccgtctatat
    18541 ttactcacga agttgcatct acaacgaatg aagcattact ttacgagatg cttataaaag
    18601 aagctaaagg caatgatgaa aaagcttatt atataacaca gtatttagac ttgataaaag
    18661 atactcttta tacacaaact atgtatgctg aatttgaaaa aactatacac gagttagtag
    18721 aaaaaggaaa aagtgtaaac gcattagtat taaatgatgt ttggggacaa cttcttaaaa
    18781 aatattatgg acaaagctat gaattagacc aattatctaa ggtcggatgg gctagaatcc
    18841 cacattttta taatagtttc tatgtctata aatatgctac aggttgtagt gcaggagtaa
    18901 gttttgccca agacatccta aagaatggtt cagataacta tatgagcttt cttaaaaagg
    18961 gaagttcaga ttatccaata aacttgttaa aatcaagtgg cataaattta actaatacaa
    19021 aacccataga aaatacaatc aaaaaatttg atagtttagt agcagaactt gaaaagctat
    19081 tataaacatt attgtaatcc acatggtaaa tatatcgaat taatgataaa atatactaaa
    19141 agtttaaagt cctatcaaga taggactttt tatttattaa ttagtgggag atgattctat
    19201 gcaagttaaa gttaaaaagg ctgtaatacc agcagcaggt ttaggaacta gatttttgcc
    19261 agctacaaag gcacaaccaa aagagatgtt accaatagtt gataagccaa ctctacaata
    19321 cataatagaa gaagctgtgg cctcaggaat agaggaaata ctaataataa caggtagaaa
    19381 taaaaaatct attgaagacc actttgataa atctgtagaa ttggaacttg atttagagaa
    19441 aaaaggtaag aaagaattgt tggaggtagt acaaaacata tctaacatga ttaacataca
    19501 ttatataaga caaaaagagc caaaaggatt gggagatgca atttattgtg cgaggcattt
    19561 tataggtgat gagccgtttg cagtaatgtt aggggatgat attgtagata atgatgtacc
    19621 ttgtctaaaa caactaacag atgcatatga ggaatataga actacaatat tgggtgttca
    19681 aaaggtaaat caagaagata ctaataaata tgggataata gaagcaaaaa atatagaagg
    19741 tagagtttac aaggtaaaag acatggtaga aaagccagag tcaggaaaag ctccttcaaa
    19801 tattgctatt ttaggtagat atataataac tcctgaaatc tttgatattc ttaaggattt
    19861 accaccaggt aaaggtggag aggtacaact tacagatgct ttaaaaatat tatctaagaa
    19921 agaagctatg tatgcataca attttgaagg caagagatat gatgttgggg acaagctagg
    19981 atttttagaa gctactgttg actttgctct aaagaaagaa gaccttaagg aagattttat
    20041 aaaatatttg aaacatgtat gtagtgaatt tgataaaaat aacaatgtgt tatacaataa
    20101 tacagatgaa aaaatagatt taattgaagt caatgaagct agagaacttg aaattcaaaa
    20161 ataattaatg aaaggtgatg tatggtggca aaatcaagtc agaaaagaat taatagaata
    20221 atagctgttg ggattgtggg aatcctcgta ataacgagta taacagctgt tttgagtatg
    20281 attgcaacta tgtaataaga taaatgacag cagttgatat acataaatat aaaaaccata
    20341 tataacttga tgtagtcaaa aacttacaat attttagtta tatatggttt ttagaattta
    20401 taaatgtaaa aaaaaatttt tataatagtt gcatatttta aaggaaaata ttattataag
    20461 atataaatat agtgataaat ttataaaaac gtgctaagat tggacttgaa atacagtaat
    20521 atagtaggta ataatgtttt aaattttatt tattgaccat aatatataaa taatgatatt
    20581 attagactta actgtttaaa taaaactttt ctttttcatg ccttcaattt ccaaaataag
    20641 tatgtatata aatgaatata tagatgagga ggattttttt gattttagat gataaagtat
    20701 tagaaggatt aggtagtaag accttagttg agctacgaga gatagctaaa gaattaaaaa
    20761 taaaatcaat tactacatat aagaaaaatg agttaattga gattataaat tccaaaagta
    20821 aaaatgagga aaaagtaaac gaagagacta aagaaaaaaa tgaagatatt aaagaagaca
    20881 taaagaatag aaaagaaaat atagagcgtg aaagtataga aaaagaagtt gaatataaga
    20941 gccctaccaa atcatacaac aagaaagaaa tagataccca aaatatttcc caaaaccaag
    21001 aaaatagaaa tcaaagaaat gactataata agacaacgag tgtaaatgat agtaataagt
    21061 catcaaatat atctaataga atggtcagaa ataataataa aaactattat atgcctaaac
    21121 aagttgatga atctaagata gtagatgaat ttaacacatc aaaagaggat gaagtagtag
    21181 gtgtacttga aattctgcca gatggatttg gttttttaag aggttcaaat tatttgtcta
    21241 ctgaaggaga tgtttatgtt tcaccatctc aaataagaag atttaatatg aaaacaggag
    21301 acaagattaa gggaataact agacatccaa aaagtggaga gaaatttaga gcacttttat
    21361 atgttcaaaa aataaatgat gaaaaccctg acacagctat acaaagaaat gcgtttgaga
    21421 cattaacacc aatatttcca gaagaacgac ttacactgga aactaataga aatgaaatag
    21481 ctacaagaat tatagattta atttcaccta taggaaaggg acaaagagga ttgatagtag
    21541 cacctccaaa ggctgggaaa acagttcttt taaaaagcgt tgcaaatagc atagctaaaa
    21601 atcatccaaa tgttgaactt atagtactcc ttatagatga aagaccagag gaagttactg
    21661 atatgaaaga atctattgaa ggagacgtta tatattcaac ttttgaccaa gtatcaagtc
    21721 atcatgtcaa ggttgcagag atggtgttaa atagagctca aagattagtt gaacatggta
    21781 aagatgtagt aattttacta gatagtataa ctagattggc aagggcatat aaccttacaa
    21841 tatctcctac aggtagaact ttatctggag gtattgaccc tggtgcactt catggaccta
    21901 aaaagttctt tggtgcagca aggaatatta gacagggtgg ttctttgaca atattaggaa
    21961 cagcattggt tgaaactggt tctaggatgg atgatgtaat atttgaggaa tttaaaggta
    22021 caggaaatat ggagctacat ttagatagaa aattagcaga aaaaagaata ttccctgcaa
    22081 tagatattta caagtctggt acaagaagag atgatttatt gcttgatgat gaagaaaaga
    22141 ctgctctttg gagattaaga agagaaatga gtaataattc agtaatggaa attacagata
    22201 aagtcattga acttataaag agaactaagg acaataaaga gtttgtaaaa tcaataaaga
    22261 atttgtaaaa tcaataatac aaatgcaaat atggattata taaactaata gaaaaatcac
    22321 ttgaatggca taataagttg tgctataata atgtagttga taatgtaaaa agaaagttaa
    22381 tataattaat ttttttaaat aaagagaaga ggtgactata atgcaaaagg aaatacaacc
    22441 aaaatacaat ccagttgaag tgcgttgtgc ttgtgggaac acatttgttg ctggttcaac
    22501 taaggacgaa ataaaagttg aaatatgttc agagtgccat ccattctata caggaaaaca
    22561 aaagaacata gaaaaaggtg gaagaatcga caaattcaag aaaagattca aaatggacta
    22621 atttttataa aagtggagtt ggaggaagcc aactcctatt ttattacaaa aaaacattca
    22681 tattctactt taaattgtct ttttgtatta taataataaa tatgtaaaat tgagtattct
    22741 aaatacaata aaatgaggtg agtggatggc aaaacaagct gtaggtgggc aagctgttat
    22801 tgaaggtgta atgatgcagt caaaagacaa aagagcggta gctgttagaa aaagtgacgg
    22861 agaaatagca ctaaaagaag atagaataaa aagttgggta agagataaga atatagataa
    22921 gatacctttt gttagaggtt cttttgttat gatagataca atgattcaag ggataaaaag
    22981 tttaaacttt tcttcagagt tttttatgga agaagctgaa gaggataaat ttgatttatt
    23041 tataaagaaa atttttaaag ataaggcaaa tgatataatt ataatatttt cattggtcat
    23101 agcaatgtta ctttctgctg gattattcat ttttatacct acattggtag gaggagcttt
    23161 ttctaaagta atgcctaatg attttatgct taatttaata gaaggaatca taaggatagc
    23221 tatattattt gcatatatag tattgatttc acgaagtaag gatatagaaa gagtctttca
    23281 atatcatgga gcagaacata aatctatata ttgctatgaa aatgatttag aacttacagt
    23341 agagaatgct agaaaattta aaagattaca tcctagatgt ggtacaaatt tcctatttat
    23401 agttatggct gtatcaataa tattgtttgc attttttggt tggccaaatc caattcttag
    23461 aatatttatg agaattatat gtgtgcctat agtagcaggt ttatcttatg aggtaattag
    23521 agttcttggg aaatatgata atggatttac taaaattatt gcatatccag gaatgatgtt
    23581 gcaatatttt acaacaaaag agcctgatga tgagcaatta gaggttgctt tggaagcatt
    23641 gaaagcagta gtagattaga gaaaaggata tttgttatga cgataaaaga tataattatt
    23701 aaatattcag ataaactaaa agatataagt gatactccaa gattagatac agagttattg
    23761 ttacaaaaaa cattgggtgt tgatagatta tatattcact taaatctgaa taaagagcta
    23821 actgaagaac aaaaaacaaa atttatgggt tttgcagaag aaagattaaa tggaagacca
    23881 atagcatata tagttgaaaa tagggagttt atgggattag atttttttgt taaggaaggc
    23941 gtattgattc caagaccaga tacagaaact ttagtagaag aaataattga aatatgtaga
    24001 gaaaaaaaag atgtaagtat attagatata ggaactggtt ctggagctat tacaataagt
    24061 ttagcaaagt acattgaaaa ttctaaaatc atgtcttttg atatatcaga gacagcatta
    24121 gaaatagcta aaaaaaatgc tattataaat gaagttggtg aaaaaataaa gtatattaat
    24181 tctgatttat ttacagcaat aagtgatagt aacataaaat tcgatatcat agtttcaaat
    24241 ccaccatata ttaaaaaaca agatatagaa actctgcaca aacaagttaa agattatgaa
    24301 ccatataatg ctcttgaagg tggagaagat ggattagact tttatagaag aataacagaa
    24361 caaggtaaga aatatttaaa taagtgtgga atattagcat atgaagttgg tcataatcaa
    24421 gctgaagatg taataaatat tatgaaaagt aatggatata aaaaaatata cacaaagaag
    24481 gatattcaag gcattgatag agttgttata ggttacaata tatgatataa tcaacaattg
    24541 atatgttaca tttaatcgag gtgaaagtat gttaaaaaaa ttagaggtat tggaagatac
    24601 atataaagac ttaagtgaaa aaataggtga tccggatgta ataaatgatc aaaaagtttg
    24661 gcagaaatat ataaaagaac atgctgactt ggaacctatt gttatgaaat acagagagta
    24721 taaaagcgtt ttagatagta taaaagaatc aaaagaaatt ttacaagaag agtcagatga
    24781 agaattaaga gaattagcta aaatggagtt agctgagatg gaagaaaaag tagcacctct
    24841 ggaggaagaa ataaaaatac tattattgcc taaagaccct aatgatgata agaacgttat
    24901 agttgaaatt agaggtggag ctggaggaga tgaagcagct ctatttgcgg gtgatttatt
    24961 tagaatgtac tcaaggtatg ctgaaagaag aagatggaaa atagaattat tgagtgcaag
    25021 tgatactgga gttggtggat ataaagaagt atcttttatg ataaaaggta aaggtgctta
    25081 ttcaagatta aagtatgaat ctggagttca tagagttcaa agaataccat caactgaatc
    25141 tggtggaaga atacacacat caacttctac tgttgcagta ttaccagaag ttgaagatgt
    25201 agaggttgaa ataaatccaa atgatttaag aatagatgtt ttccgttctt cgggtaatgg
    25261 aggacaaagt gtcaatacta ctgactctgc tgttagggta acacatatac ctacaggtga
    25321 agtagtatct tgtcaagatg gaaaatcaca attaaaaaat aaagagcaag ctttaaaaat
    25381 attaaaagct agactttacg ataaagcatt ggcagaacaa cacaaagata tagcagcaga
    25441 aagaaaaagc caagtcggaa caggagacag gtcagaaaga ataagaacat ataatttccc
    25501 tcaaggtaga ataagtgacc atagaataaa tttaacttta tataagttag atgcattttt
    25561 agatggagat atagatgaaa tgatagatgc tttaattact gttgaccaaa cagaaaaaat
    25621 gactgcaata taagtaatgt tttaaagaag ctgtttcttc ctagaaatta ttttctattt
    25681 tgaaacagct attttgcatt ttaggtcatg ttatcatatt ctattgtata taattgttat
    25741 atacaagtat aggagggtta tatgatacta aaagtaacaa taattgggct tttggcaggt
    25801 gttataggga caggattagg aggagtaata tcagccattt ttaaaagaga ggtcgataaa
    25861 tatctaagtt tttttatggg attatctggt ggaataatgt tggctgtagt ggtgtttgat
    25921 ttaatgaaag aatccatgga caaaatgggg ataatcaata cagttatatt tacgtttgtt
    25981 ggagctctca taactatgta cataaaaaca aaattagatg tgtctggaaa tatggcatct
    26041 ggatatctta tattcataag tatattacta cataatttac cagaaggact agccattggt
    26101 tcttctttta tgtcaactga aagtttaggt attacacttg ctatagttat aggtctacat
    26161 aatataccag aagggttagc tatggcatta ggcttagttt gcaataaaat gaaactaagt
    26221 aaagttattt tgtttactgt aatagctgga ttacctatgg gtctaggaag ttttttaggt
    26281 gtttattttg gaggagtatt tacttctctt ataggagtat ttcttgctac agcaggaggt
    26341 actatgatgt atgttgtatt ggaagaaatt tttccacatt caaaaagtgt gtattgtata
    26401 attggtttct tgttaggtac aatgattgta aattatatat aaaattaaaa tgaaagcagg
    26461 catacaaaag atgaaaacta ttataagtaa tattgatata aataatattg attatgagga
    26521 aataaaaata caagcaaaac ttcttagaga aggtaaaaca gttatttttc caactgagac
    26581 agtatatggg ttgggtgcaa atgcattaga tgaaaatgca gtaaagaaga tttatgaagc
    26641 taaaggaaga cctagtgaca atccattgat agtccatata tatgaaaaag aagaagtata
    26701 tgacttagcc aaagatataa gtgataaagc taaacttgtt atagaaaagc tttggccagg
    26761 acctataaca attatattaa ataaaaaaga tataattcca tacaaaacta gtggagggct
    26821 tgatacagtt gcaataagga tgccttctaa tgttattgcc agggcaataa taaaagaagc
    26881 tggtatacct attgcagctc cttctgcaaa tatctcaggt cgcccatctc caacaaaagc
    26941 aaaacatgta tatgaagaga tgaatggaag agtggatgga atagttttag gaggagacag
    27001 taattttgga ttggagtcaa ctgtattgga tttaacagaa gagactccta tgatactgag
    27061 accaggaagt ataactaaag aagttttaga ggaacttctt ggagaagtaa agcttgaccc
    27121 atcattaagt aaaaaagaag ataatcaaaa agctaaagct cctggaatga aatataaaca
    27181 ttattctcca aatgcagatg tatatataat atctggaaaa agagaaaatg tagcaacaaa
    27241 gattaatgat atgataaaat ctaatagaga caaaggtcta aagacaggtg taatgtgtat
    27301 cagtagaaac cgagagtttt atgatggaga agtaatcgat ttaggaaact ctcttgaaga
    27361 agttgcaagt aatctgtttg atgctcttat agaaatggac aaaaagggcg ttgatgtcat
    27421 atattcagaa gagttcccta agactggagt tggacaagca attatgaata gactactgaa
    27481 gtcagcagga tacaaagtga taaaggctta agagggagtt gtaaaatatg aatatactta
    27541 ttgtgtgtac aggtaatact tgtagaagcc ctatggctga ggctatacta aggaaagcaa
    27601 ttaaagaatc tggaagaagt attgaagaat attcgatatc atcagcaggt atatctacag
    27661 caaatggaat gggtgcttct gaaaattcta tagaagtact aaaagaaatc ggtatagact
    27721 tatcaaatca tagaagtaaa gtaattacta aaaaattaat tgatgaatca gatataatac
    27781 taacaatgac taaatctcat aaagaaattt tagtacaagc agttccaaaa tgcaaagaaa
    27841 aagtgtatac ttttaaaggg tttgcaaata aaaatgagga agatatatct gacccatttg
    27901 gtggaaattt ggatatttat agaagcacta tgagagaaat aatgtattct gtcaacgaaa
    27961 tagtaaaaaa aatatgaaac ggaggataaa caaatgaaga taggattagg ctgtgaccat
    28021 ggaggatata atttaaaaaa agaaataata agctatttag aaagtaaagg tatagaatgt
    28081 gtagattatg gaactaataa tgctacagat tctgttgatt atcctgttta tggagaaata
    28141 gttgcaaatt cagttataaa taaagaagta gattatggaa tattatgttg tggtacaggg
    28201 ataggaatat ctttagctgc taataaggtt cctgggataa gatgtgcagt tgtatcagat
    28261 gtattttctg caaaaatgtc aaaagcacat aatgatgcaa atatgttatc attaggcgaa
    28321 agagttttag gaaaaggtct agcacttgag atagttgaag catggattaa tacggatttt
    28381 gaaggtgata gacatgctag aagagtaaac atgattaaat caatagaaga aaaacataat
    28441 aaataataaa aattacagtt ttatcaggag gaattatcaa aatgagcaaa gtggtagaga
    28501 caaaccatcc attaatacaa cataaattaa ctttaatgag agataaaaat actggttcta
    28561 aagactttag agagctttta actgaaattg caatgttaat ggggtatgaa ataacaaaag
    28621 atataccatt aaaagatgta gaaatagaaa ctcctattca aaaaacatca tctaaagtag
    28681 ttgcaggtaa aaaattagca ataattccaa ttttaagagc aggtcttgga atggtagatg
    28741 gattagtaag cttaatgcca gcagcaaaag ttggacatgt aggactatat agagacccag
    28801 aaacattaaa accagttgaa tattattgca agcttcctca agacatagga gaaagagata
    28861 taatagtagt tgaccctatg cttgcaacag ggggttctgc tgtagcagct atagatttac
    28921 ttaagtctaa aggagctaaa agtataaaat tagctaattt agtagctgca ccagaaggta
    28981 tagcagaagt gcaaaaatat catgatgatg ttgatatata tgttgcatca gttgacgaaa
    29041 gattaaatga acatggatat ataattcctg gtcttggtga tgctggagat agattgtttg
    29101 gaactaaata aaaataaata actataaata tttattaggg actttcttat atagtgtata
    29161 taattttatg taagaaggtt cttttttata tattttttct caaaatagga attatgaatt
    29221 gataaatttc tcgaattgag aataaaaagt ttttaccaat taaaaaagat tgcttatttt
    29281 acaaggtttt atagaatagt agtctatttt aatgtggcat gttagttgct tacgattaag
    29341 attataggta ttaagtgtat tagttaaatt gagaattgaa tattaataat attattggga
    29401 gtggtaatac atgaagaatt gtatagcagt aagaggggct ggagatttag caacagcagt
    29461 tattcataaa ttacacaaca gtggttttaa agtagttgcg ttagaaatag aagagccatt
    29521 agctataaga aggcaaattt ctttttgtga ggctgtttat gaaaaaaaag taactgttga
    29581 aggtgtagaa tgtatatttt gcaatactga aaaagaaata gaagaagttt tatctcaaca
    29641 taaagtagcc ttgattatag acccaaaagg agaaaaatta tctaagttaa atccaatcgt
    29701 tttggttgat ggaatactcg ctaagaaaaa cttaggtaca actaaagata tggcaccact
    29761 tacaatagct ttaggtcctg gattttgtgc aggagaagat gtagatgttg ttatagagac
    29821 aatgagaggt cataatctag gaagaataat aactaatggt agagcagaaa aaaatacagg
    29881 agtaccagga gcaataaaag gtttttctaa agagagagtt atgcattcac agtatgatgg
    29941 tataatggaa aatgtatgta agataggtga tatagttgaa aagggagacc tactttgtta
    30001 tattaataat tatgaaaaaa aacatgaagt tagagcaaca atatctggag tccttagagg
    30061 tttattaaag gatggagcaa aggtgaacaa aaatttaaaa atactagaca tagaccctag
    30121 aaaagaagaa gttaagaatg cttttacaat atcagataaa gctagatgta tagctggagg
    30181 ggttttagaa gccatactaa acaatgggat ttatccccaa aattaattta tattagataa
    30241 agctattaat attttattaa gatgatataa taaatttaat agattaaaga gagcatatga
    30301 cgggtattta aaatttaaat ggtaaaaatg ctcttttttt attggacatt caattaacat
    30361 gtgtatataa tataaaaata agtatttatt taggttgaaa tctaataaat atataatacc
    30421 agtacataag caaagaggag aaaagtaaat tatgagacca tcatgggatg aatacttcat
    30481 ggaaattgca gaagtagtga aaaagcgttc aacttgtatt agaagacaag taggagctgt
    30541 aattgtaaga gacaaacaaa tattaacaac tggatataat ggttctccga gaaatcttga
    30601 acactgtgaa aatattggat gtaagaggca ggaattaaat attccctcag gagaaaggca
    30661 tgaattatgc agagcattac atgctgaaca aaatgcgata atacaagctg ctcataatgg
    30721 tattagtgta gatggggcaa ctttgtatgt tactacaaga ccttgtgttt tgtgtgcaaa
    30781 aatgtgcata aatgcaggta tattgaaaat tgtttatgaa ggtgattatc cagatgatat
    30841 gtctacagaa ttattaaaag aagcaggaat agaactcata aaattttagt ggataaaatt
    30901 tatatgacaa taaatgtcaa attatgacac tgaaatacaa tggtatcaaa tgattaaaag
    30961 aaagtatttg aaaaatcatc agattagtag taagatatat tttataatta aataaattta
    31021 tgagatagat taattacatg ttatttattt tataataatg agggatacta tttatgagta
    31081 aaagaaatac acatacagaa attgcaaagt tgtttagttt aataagtcaa ataggattga
    31141 tgattgtaat atcaatatta ggatgtactt tcttaggaaa atttttagat agtaaattga
    31201 atactactcc agttctgaca ataatatttt tattgttggg tgttggaggt gcttttagtg
    31261 gtgtttataa aactctaata gtatatacac gaaggaagtg atatattgga tataaaatta
    31321 cttaaccagg taaggtcagt aacaaaaggt atagtaatat ttgatgtagt tttaattgtg
    31381 ctaatgttga taacatcaac attaagtaaa ccagtattat taggacttat atttggctct
    31441 ataatagcta tgttaaattt tagattattg gcgatatcac tggaaaaatc agtgacattt
    31501 cctgctggga aggcacaagc ttatgcatca gcacaatatg ctattagatt atttataatg
    31561 gcagttgttc taattgcatc agctacagta ccacatttaa atataatcgg aacagcattg
    31621 ggattattga gcacaaagtt tgtcatatta tcaaaaaact ttataaataa attaaagagg
    31681 aaggaggcat aagtatgaat caagcgcatt attatctcat aggaaacttt aagattagtg
    31741 aaacaatact agtaagttgg ggaataatgg cctttttagc gatagcatct tattttttga
    31801 ctagaaactt aaaaaaagtt ccaactagta aagttcaaat attcttagaa tttgcagtag
    31861 gtggtcttgc taaaatggtt acagatgtta tgggtaaaaa tacagtaaaa agaatgccat
    31921 atatgatacc atacatagga agcttgtttt tatttttcgc atgttcaaat ttaagcccat
    31981 tacttggatt aaggtcacca acaaccgact tagacacaac tgctgcatgg gcaatgatta
    32041 cattttttat gatttatttt gcaggagtta aatttaaggg gttatcatac tttaaagaat
    32101 tggctcaacc acttgccata atgttaccaa tcaatattat aggtgagtta gctagaccaa
    32161 tatcattaag tttccgtcca tttgggaata ttttaggtgg aacaataata atgggtctgt
    32221 tttatcaact tttaggtttt atatctagtt taataccagg attatcaata ccaattggag
    32281 aaatattaat accagtccca ttacacttgt actttgactt atttgcagga ttgttacaag
    32341 catatatatt tataatgtta acaatggtat ttatatcaag tgcaggagag ttagattaag
    32401 aaatcaactt atattaagat aaaaataaaa taatttaaaa aattaaagtt ttaaggagga
    32461 aataattatg gaaacagcaa tagtagcggc agcatcagca ataggagcag ggatagcagt
    32521 tgcaacaggt ataggtgcag gtataggtca aggtatagca gcagctaaag ctgcagaagc
    32581 agtaggaaac caaccagaag ctaaaggtga tataacatca actttattac taggagttgc
    32641 tatagcagag tcttcagcaa tatatggttt ggttatatca ataatacttc tattcgtaaa
    32701 tccattcttt aaatacttag gaatgtagat ttagtcctta aatattttga gataggttta
    32761 taggtaaatc atctcaaact tataatcatg agatgaactt tatcatctcg tgattataag
    32821 tagttatgat ttatggaaag gaggataatt atggaaaaag cactagtagg tataacgtgg
    32881 gaatttgtgt tccaaattgt taatactttt ataatatttt tacttctgag aaagctttta
    32941 ttcaagccag tattaaatat aattgaatca agagaaaatg atattaaaag tgatttggca
    33001 gaaggcgaaa aagcaaaaaa tgaaggttta gctctgaaaa aagaatatga gtcaaaaatc
    33061 aattttgcca aggatgaagg tcaagaaatc ataaaacaag caactataag agcagagcag
    33121 aaatcagatg atatagtaaa tactgcaaag aaagatgctc ttgatataaa agaaaaagca
    33181 aacaaagata tagaacaaga aagacaaaag gttataaatg aaatcaaaaa tgatatatct
    33241 aacatagcac tacttgcagc atctaaagtt attgaaaaag atttagataa atctaaacat
    33301 gaagaattga tagaaaactt tataaaagag gtaggggaag ctaaatgata aatgtaatag
    33361 caaatagata tgccgaagcc ctatttcaat taggagaaga agaaaattca actgatgtct
    33421 tattcaaaga actagaaaaa gtagtagata tgatgactaa agttagtaaa gatttctaca
    33481 aagttttaaa atcaccatta gtttcaaaat cagaaaagaa aaacttggta gaaattattt
    33541 ttagtaagga agtaagttct aacataaaaa actttttaaa agttttagta gataaagata
    33601 gaatatcata tttagaagat atcgaattag cttataaaga attattaaat aagaaaaaca
    33661 atgtaattga tggagtagca ataagtgcta ttccaatgag tgaaacagat attaaagaac
    33721 ttgaagtcaa actttcaaat aaatacaaca aaaatgttac tatagaaaat gttgttgaca
    33781 agactatact agggggagtt ctagttagaa ttggaaatga acaaatagat ggcactgtga
    33841 aaactcgtct tgataagatg aaagaaaagc tatctgaagt aatatcttaa gaatggcggt
    33901 gaacccatga acttaaaacc tgaagaaata agttctataa ttaaacagca aataaaaaat
    33961 tatgagaata aagttgagtt gacagataca ggtagtgttt taacagttgg ggatggtata
    34021 gcgagtgtat atggattaga aaaagctatg tctggtgagt tgctagagtt cccaggtgaa
    34081 atatatggta tggcacttaa cttggaagaa gaagtagttg gagctgttat ccttggagat
    34141 gacagtgaaa taaaagaagg cgatatagtt aagagaactg gaagaatagt tgaagttcca
    34201 gttggagaag ctttaatagg tagagttgta aactcactag gtcagccaat agatggaaaa
    34261 ggacctatag cgtacacaaa aactagacca gttgaatctg aggcacctgg tataatagat
    34321 agaaggtctg tttatgaacc attacaaaca ggaatcaaat caatagattc aatgatacca
    34381 attggtagag gtcaaagaga gttaataata ggtgacagac aaactggtaa aacatctata
    34441 gttattgaca ctatactaaa tcaaaaagga aaagatgtta tctgtatata tgtagcaatt
    34501 ggacaaaaac gttctactat agctcaatta gttagctctt tagaaaaagg tggagctttg
    34561 gattatacaa tagtagtttc agcaacagca tctgaatcag caccacttca atatatagca
    34621 ccatatgctg gagcagctat gggtgaagaa tttatgtaca atgggaaaca tgtacttata
    34681 gtgtatgatg atttaagtaa acaagctgtt gcatatagag aaatgtcatt gttacttaga
    34741 agaccaccag gacgtgaggc atatcctgga gatgtattct atttacattc aagattactt
    34801 gaaagagcag ctaagttatc tgatgaattg ggtggaggtt caatgactgc cttgcctata
    34861 atagaaactc aagctggtga cgtttctgca tatataccaa caaatgttat atctataaca
    34921 gatggtcaaa tatacttaca accagagtta ttctattcag gtgtaagacc agcagttgac
    34981 cctggtatat cagtatcaag ggttggtggt tctgcgcaaa ttaaagcaat gaaaaaagtt
    35041 gcaggaacat taaaacttgc atattcacaa tatagagaac ttgcagcatt ctcacagttt
    35101 ggttctgact tagatgaaga tactaaaaag agacttgctc aaggtgaaag aatcgttgag
    35161 atattaaaac aaggtgagca tcaaccaata aaagttgaaa atcaagttat gataatatat
    35221 gctgttataa ataatcattt agaggatatt ccgatagata atatagctag atttgaatca
    35281 gaattgtatg catttgtaga taataattat ccggaaatat caagaaaaat attaggtgga
    35341 gaagacttca ctcatgattt aacagatgca ataaatgaat ttaaagaaaa gtttgttgtt
    35401 gaagtataat cctttttaaa aaaggaggta aattaattgg caggagctgg aatcaaagca
    35461 atcaaaacac gtatgggaac tgttgaaagt acaaagcaaa taactaaagc aatggaatta
    35521 gttgcatctt ctaagcttag aaaagcaaaa gaaaaggctg aagattcaag agaatatttt
    35581 aatactttat acaatacagt aaaagatatt gcccaaacaa cacgtggaat tagaaatgaa
    35641 tttttaaaag aaagagaagt aaaaaataaa tgttatgtag tagtagctgg agacagagga
    35701 cttgcaggtg gatacaattc caatgtatta aaggaatctg tctctcatat gaaaggtaaa
    35761 aaggaaacag tgattactgt tggaaagaaa gcacatgaat ttttttctaa aagaaactac
    35821 aatatagcaa aagatataaa tagtgttgaa gcttgtggat atgaagatgc gacagaaatt
    35881 tcacaaacta ttattgacct gtataaaaaa ggtgaagtag atgaagttta tattgcttat
    35941 acaaaattta aatctgcttt atcacaagaa gttgaaattt tgaaattgct tccattagtc
    36001 tttgaaaaag atacagacga acaaggaatg gcagtaaaag aagaaaaaca aaatagatca
    36061 aaagtacaat atataccatc agcagaagca gttttaagtt atattgtacc tcagtttata
    36121 tcaggtacag tatatggtgg aattttggaa tcttttgcat cagagcaagg tgctagaaga
    36181 actgcaatgg aatcagccac agacaatgcg aatgaaatgc tatctaattt agagttaagt
    36241 tataacagag caagacagtc agctgtaact caagaaataa cagagatagt tggtggagtt
    36301 gaagctctaa aatagggagg ttaggaaatg gcaaatgtag gtaaagtagt acagattgta
    36361 ggagctgtac tagatgttaa gtttgatagt gaacaaagcc tacctaactt attaaatgcg
    36421 ttagtaataa agttaggaga taaagaaata gttgcagaag ttgctcaaca tataggtgat
    36481 gatactgtaa gatgtatagc tatgagtgca acggacggac ttgtaagagg tatggaagtt
    36541 gtagatacag gagggccaat aagtgtccct gttggagatg agactttagg tagaatattc
    36601 aatgttcttg gaaaaccagt tgatgggaaa ccagctccaa aatctgcacc taaattacct
    36661 atacatagac cagcaccggc gtatgatgaa ctagaaacta cagcagagat actagaaact
    36721 ggtattaagg tagttgactt actagctcca tatctaaaag gtggaaaaat aggtctattt
    36781 ggaggagcag gagtaggaaa gacagttcta atacaagagc ttataaacaa tattgctaag
    36841 caacatggtg gtatttctgt attttcagga gtaggagaaa gaacaagaga aggtaacgac
    36901 ctttatggcg aaatgagtga gtctggagtt ataaataaaa cagctctagt atttggtcaa
    36961 atgaatgaac cacctggagc gagaatgaga gttgctttaa ctggacttac aatggcagaa
    37021 cattttagag atgagcaagg acaagacgtt ttacttttcg ttgataatat attccgtttc
    37081 acacaagctg gttcagaagt ttcagcactt ctaggacgta tgccatcagc tgttggttat
    37141 cagccaacat tagctactga aatgggtgca cttcaagaga gaataacatc aactaagaaa
    37201 ggttcaataa catctgttca agcagtatat gtaccagcag atgacttaac tgacccagca
    37261 ccagctacaa cattctctca cttagatgca aaaacagttt tatcaagaca aatatcatca
    37321 ctgggtatat atccagcagt tgaccctctt gaatctacat caaggattct tgaccctagt
    37381 atagtaggaa aagaacatta tgaagtagct agaggtgtac aatcaatact tcaaagatat
    37441 aaagaacttc aagatataat tgctatactt ggtatggatg aattatctga tgaagataag
    37501 ttaatagttg cacgtgctag aaagatacaa agattcttat ctcaatcatt cacagttgct
    37561 gagcaattta caggaaatcc aggtcaatat gttcctgtta aagagactgt aagaggattt
    37621 aaagaaattc ttgaagggaa acatgatgac ttaccagagt cagcattctt atttgtagga
    37681 acaatagaag acgctgttag aaaagcgaag ggaagtatgt agtcatggcc agtgaatttt
    37741 cattgaaagt tgtaactcca agtaatactt tttatgatgg agatgttgaa atgatagtag
    37801 ttagaactac aaaaggagac aaaggggtgt tgaaaggtca tataccattt gtttcaggta
    37861 tacctgtagg aactttaaag ataaaaaaag agggaagttt taaagaggcg aaaatagcag
    37921 aaggattcat taatgtaact caagataaaa ctgtcatatt gacagaatct gctgagtgga
    37981 tttaataaat taatttagag tataaaaagt cattttgacc aaaaaaactt ttgggtaaaa
    38041 tggctttttt gtgttttggt gttaataaaa taacgataaa attgtaagtt tttagtgata
    38101 ataaattcac aaaatgttta aatttactgc caaattatgt tgtaattaat aagataaaga
    38161 agatttagat agcttttatg taagtgaaaa agtcttcata taagaaatac atataaaatt
    38221 tttataaaat ttaaaaaatt tgctt
//
LOCUS       DAEPOZ010000009        37297 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.009, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000009 DAEPOZ010000000
VERSION     DAEPOZ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37297
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.009"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(127..288)
                     /locus_tag="KR440_000396"
     CDS             complement(127..288)
                     /locus_tag="KR440_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697465.1"
                     /translation="MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFD
                     FKIKFHKFK"
     gene            complement(629..1069)
                     /locus_tag="KR440_000397"
     CDS             complement(629..1069)
                     /locus_tag="KR440_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBH0697466.1"
                     /translation="MSNLSAFLSQNAIKVDNVKYVASNRFLDKEGKPVEWELKVLSSE
                     EDEALRRKCTKRVKVIGNNGKHTGQYTSEIDYNSYVAELCVASTVFPDLKDAELQNSY
                     GVMGEAQLLKTMLTAGEYVNYTVKVNEVNGFDTSFEDKVEEAKN"
     gene            complement(1141..1968)
                     /locus_tag="KR440_000398"
     CDS             complement(1141..1968)
                     /locus_tag="KR440_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697467.1"
                     /translation="MEFESGIKLGKLTLIKYLGRNKHSKKVWLCQCECGNKVERVENN
                     LKQSIKNEKPPHCGCSPNWKGQNKRFKDITGKTFGKLTVLKMSGKDKYSHNLWLCQCE
                     CGNRTITSTSALEQGKAQSCGCIRKEILLERSTTHNKSNDSLYRVYHRMIKRCTNKSN
                     KDYNYYGGRGIEVCNEWLEDFINFYNWSIENGYRKGLTIDRINVNGNYEPSNCRWVTW
                     EVQRNNKRNSIRVNYKGEMITLKQCAENLNVKYLFLYNQLVTKKIPLEEVIKNIGME"
     gene            complement(2027..2497)
                     /locus_tag="KR440_000399"
     CDS             complement(2027..2497)
                     /locus_tag="KR440_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBH0697468.1"
                     /translation="MAQTINAKDTVSAKKAECFITIEGKRYNFMQAIDLEAKMEKNKS
                     EVPILGRTTKGNKTTGSTNTGSATFHYNTSIFRELLYRYKETGEDIYFDIQVTNEDPT
                     SAVGRQTVVLKDCNMDSGIITKFDADGEYLDEDMDFTFEDWELVESFALLNGML"
     gene            complement(2514..3824)
                     /locus_tag="KR440_000400"
     CDS             complement(2514..3824)
                     /locus_tag="KR440_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HBH0697469.1"
                     /translation="MALGGGTFVTQNKILPGAYINFISAKRATSSLSDRGIVAMPLEL
                     DWGIDEEAFQVTSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIATAKYSGIRGNDLKVTVTTNIDDNAKFDVVILLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKSDADYEGVVSIENKIKDKDLVESSLIYWATGAIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNEKYLGKVPNDKAGRISFWNDVVKHHKELENI
                     RAIEDFKTDDVSVELGNDKKTVIVSDAIKVINAMSKLYMTVSVN"
     gene            complement(3825..4001)
                     /locus_tag="KR440_000401"
     CDS             complement(3825..4001)
                     /locus_tag="KR440_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697470.1"
                     /translation="MSKALSKEDNYKFTKEQIVNSKKYVNRKDLLNAILKENELYSFS
                     EVEDRINKFMKGVS"
     gene            complement(3994..4431)
                     /locus_tag="KR440_000402"
     CDS             complement(3994..4431)
                     /locus_tag="KR440_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697471.1"
                     /translation="MLNNIIDGISVKLDKSFGEKYTIYSEDVEQGINEPCFFIVPLNP
                     SKTPYPSGRELKKNSFDVHYFPRSEDKNFEINEIAEMLLEELEYIEIDGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKNNDTNKMDTVELFGGLKRGDNFE"
     gene            complement(4780..5586)
                     /locus_tag="KR440_000403"
     CDS             complement(4780..5586)
                     /locus_tag="KR440_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697472.1"
                     /translation="MAKFLDITGKKFGRLRVIKFSKEIKSGKRNRKYWLCKCDCGNFK
                     EIRTDSLTSGLVQSCGCLKKEQDKLNLTDKYQFKKKYKVQNKRLYSIWKGIISRCTDK
                     NNKRYNRYGERNIIVCDEWFCYDNFANWALSNGYSEKLTIDRINNEGNYESSNCRWVD
                     IKTQCRNRSTNILVKHEEKEITLIELSEKTGISYSCLRSRYSKGLVGNKLIEKVKIIE
                     ESRAKLSIEDVKEIRKKYSDGYTIKQLSEIYPVTYSSISNIVHRRTWKNI"
     gene            complement(5696..5986)
                     /locus_tag="KR440_000404"
     CDS             complement(5696..5986)
                     /locus_tag="KR440_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBH0697473.1"
                     /translation="MARWGSVDFREFKRVCKKMEKFTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLREGWSGVAYARSLPVYKQGNNYIIEVVNPTEYALTQWGI"
     gene            complement(5986..6333)
                     /locus_tag="KR440_000405"
     CDS             complement(5986..6333)
                     /locus_tag="KR440_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697474.1"
                     /translation="MVSKTRKAIEMLYRDKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSYKNIVSATEGKLAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            complement(6327..6728)
                     /locus_tag="KR440_000406"
     CDS             complement(6327..6728)
                     /locus_tag="KR440_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697475.1"
                     /translation="MENNIIDEIEKRLESFGYILKDGDKWLIGFVREKIENIIKLDCN
                     IKTMPIELKEIEVDMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLIAYLTTYGKNKILTFRCLRW"
     gene            complement(6738..6971)
                     /locus_tag="KR440_000407"
     CDS             complement(6738..6971)
                     /locus_tag="KR440_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697476.1"
                     /translation="MPQVKKLNRILTIEECKIDDFLEMGYDLIDETGKAVKYGKSLSV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            complement(6990..7880)
                     /locus_tag="KR440_000408"
     CDS             complement(6990..7880)
                     /locus_tag="KR440_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HBH0697477.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATENNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWEPKVLTNQRKWSTLVHPADINQTNYVASI
                     GNITKVYNEEQKFPEMDAYCISKIYADWTALGNTADTTVLTTANVLEVFDKLMEKMTE
                     ARVPENGRILYVTPVVNTLIKNAKEIQRTVNIKDGGTSLNRQTTDIDTVKIIKVPSNL
                     MKTAYDFTTGWKVGAGAKQIFMSLVHPSAIITPVSYQFSKLDEPTAVTEGKYFYFEES
                     FEDVFILNKKADAIQFVVEA"
     gene            complement(7892..8455)
                     /locus_tag="KR440_000409"
     CDS             complement(7892..8455)
                     /locus_tag="KR440_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HBH0697478.1"
                     /translation="MDWLKELLEGIKVEDNKIDVASLQKSIEKKIKETTVTQEDYVNL
                     ETQLNTANETIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLGDAF
                     KSVKCRDEIALKAHLDMEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPGQEPKINDFGFNFTGVRPHENNNK"
     gene            complement(8523..8771)
                     /locus_tag="KR440_000410"
     CDS             complement(8523..8771)
                     /locus_tag="KR440_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697479.1"
                     /translation="MDNKFKNLQHLIDSIDLGLDIEFDLYNKPYNISIGDDDTRFITL
                     CPNGDTKYYKNGKDMVDNYNIDGKLLKDLWKDIQIVNM"
     gene            complement(8773..10308)
                     /locus_tag="KR440_000411"
     CDS             complement(8773..10308)
                     /locus_tag="KR440_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBH0697480.1"
                     /translation="MKHKDYWRKRFEQLEEVQNNKSVKYYLELEKKYKLAMNSIEKDI
                     LSWYNRFAENEGISLLEAKKLLNTRELEEFKWNVEEYIKYGKENAINQKWMKELENAS
                     ARLHITRLEALKLQIQQHVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWS
                     LMSLDTNRINKIISKPWATDGLNFSERIWGKHRPTLINELHTKLTQSIIRGENPKNLV
                     NDFAKRFNVSKSQAKNLIMTESAFFASASRKDCFSDLDVEKYEIIATLDLRTSNICRE
                     LDGKIFDMKDYQVGITAPPFHCRCRTTTAPWFEDEEGYRAARGEDGKTYYVPSSMKYN
                     EWYEKHIEGRLGKEKADTLRKMQLNESKDRKQFEEYKKVLGNEIPSKFDEYQHMKYND
                     TIRYEEAKKLYKDVNWQVKNQRNLTSGSVHSVPFESKPNSVFDNYKDGKLIQRRYYGN
                     TGKPRLDLDLTDHRNPKQHKIVPHKHDWLADENNHDKVKREKDMELTKAHKIANKDIL
                     NGE"
     gene            complement(10298..11740)
                     /locus_tag="KR440_000412"
     CDS             complement(10298..11740)
                     /locus_tag="KR440_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBH0697481.1"
                     /translation="MLNIYISETDLIKVQLKKESTFNLVKVIEHYILKHRPEKYKQGE
                     EYYYGNTDVNNKRRYYLLDGAKVDDFTKVNNKAINNYHKLLVDQKVGYSVGNPIVFNA
                     DDDNLTKLLNDLLGEEFDDTITELYLNASNKGVEWLHPYINRKGEFKYVIIPAEEAIP
                     IWDSKRQRELVAFIRFYYIEDIDGNKIKRVEYYTENDVTYFIERGNSFIQEFLYDEYG
                     KMTDIQEGHFRINNKEQGWGKVPFIPFKNNEKCVSDLTFYKSLIDIYDNNISTLADNL
                     DEMQEVIYVLKEYPGTSLQEFIDNIRYYKSIKVDGGGGVDKLEINIPVEAKKELLDRL
                     EKNIIIFGQGVNPESQNTGDKSGVALKFLYSLLDLKCSKTEKKFKKAIRELLWFVCEY
                     LKISGSKSYDYKSVQITFNHSMIINESEKIDMASKSIGIISDETIVSNHPWVEDVNDE
                     LERLKKQEETQKEYDDLIPTNNQEGVIDET"
     gene            complement(11746..12999)
                     /locus_tag="KR440_000413"
     CDS             complement(11746..12999)
                     /locus_tag="KR440_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBH0697482.1"
                     /translation="MVIEMIVRINFNPDFKEANFTKKRYRAMKGSAGSGKSVNVAQDY
                     ILKLGDKKYQGANLLVVRKSEATHKYSTYAELTGAINRIYGKQADKYWKTTLNPLEIK
                     SKVTGNSIIFRGVNDAKQREKLKSINFSKGKLTWVWCEEATELMESDIDILDDRLRGI
                     LTNPNLYYQMTFTFNPVSATHWIKRKYFDYKNDDIFTHHSTYLQNRFIDEAYYRRMQM
                     RKEQDPEGYKVYGLGEWGETGGAILKNYVIHEFPTEFEYFDNMRLSQDFGFNHANAIL
                     RIGFKDGELYICNEIYVHEMDTSEIIKIANSRGLEKTLFMYCDSAEPDRIKMWKSAGY
                     KAKGVKKGPGSVKAQIDYLKQLRIHVHPSCTNTIKEIQQWKWKQDERTGLYLDEPVEF
                     MDDAMAALRYSIDNKLKNNGISFLK"
     gene            complement(12977..13669)
                     /locus_tag="KR440_000414"
     CDS             complement(12977..13669)
                     /locus_tag="KR440_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0697483.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISAKYDISLNTLKSWIKRYNWSSE
                     KKKGAPINKRGAPFSNKNSVGYGAPKENKNAEKFGFFSKYLPEETRELIQEISIKDKF
                     DILWEQITIQYAAIIRAQKIMYVKDKEEMIKELKKHEITENSEKIEYEFQFAWDRQAS
                     FLNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVANLNKDDIGDDEL
                     KISVDYGDRNDS"
     gene            complement(13739..14248)
                     /locus_tag="KR440_000415"
     CDS             complement(13739..14248)
                     /locus_tag="KR440_000415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697484.1"
                     /translation="MYKKVLNILLNYYKAINSKEVVELVLTPVLICIISYMFFNSSIN
                     KEVVISLNKDVLTLSGLLVAFGVCIITLLFTTYNKSISEAKDIKTERKVNGFNISYFQ
                     FIQLKAYYTVIMEILVVVICFINTIMLTRYYSNIIFYVLIFFTVHIILSLTTLIISMF
                     HLSWKDRGD"
     gene            complement(14269..15243)
                     /locus_tag="KR440_000416"
     CDS             complement(14269..15243)
                     /locus_tag="KR440_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697485.1"
                     /translation="MSNFKKRYIDVYRIDIINKINGSVYSDEEILKEVIKTFFKDKEY
                     CKENKNGINITKDKKFWITEVKCSEDDLIIKVKLEYTKYNQNTNIINAHTKEVEGNKK
                     MDQGDSNKQHLFIKFMKENNIAVVLFERVFVGVPMIELSKNLHLYYEDMIENNKFDNN
                     MSKTSINITQIASKDFIEQILDLDGVSKITLNVDREKFGTDEDNLFSNLNNSRRHNDL
                     IYKPTFRTRYLPSEVKRCCEKYIKGEEFRNKKINRIIIEGEKNKRKLKLDTEGIKLCR
                     EIEVSLDIDNHIDSEDIFEKMNNLIDDLIENNSVFFNLMYQEVAMSEA"
     gene            complement(15438..15987)
                     /locus_tag="KR440_000417"
                     /pseudo
     CDS             complement(15438..15987)
                     /locus_tag="KR440_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
     gene            complement(16270..16749)
                     /locus_tag="KR440_000418"
     CDS             complement(16270..16749)
                     /locus_tag="KR440_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
                     /protein_id="HBH0697486.1"
                     /translation="MDKESIKEIVRELRKEEREDRKKGVLYNTRLLMKHYNDFKRHID
                     SAVSEAKDVDYLEDDLGKLDNEELYILSIKKSKARTIIMIAHIDSAMKTLKLRQERLH
                     SYEKYRALELYYCDEKTYEEIAEILNCGVVTSRRWINEMVKELGVYLFGIDGLKLTV"
     gene            complement(16742..16879)
                     /locus_tag="KR440_000419"
     CDS             complement(16742..16879)
                     /locus_tag="KR440_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697487.1"
                     /translation="MELISPGKIVRCAWCGRRFYKLDKSKVEYCSRSCSKRARRSDLS
                     G"
     gene            complement(16903..18273)
                     /locus_tag="KR440_000420"
     CDS             complement(16903..18273)
                     /locus_tag="KR440_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBH0697488.1"
                     /translation="MEFKPHPYQEYTIRKTIDNNNIGLLLDMGLGKTVCTLTAISELM
                     YDYFDISKVLVIAPLRVARDTWSSEVKKWEHLKHLKVSKVLGSKLDRVRALSTDSDIY
                     IINREMVPWIVDFYKRKWPFDMVVIDELSSFKSNKAQRFKSLKKVLPLTKRVVGLTGT
                     PTPNSLIDLWAQMYLLDRGERLGKTITGYKERYFEPGQKNYQTGAIYNWQPKNGAENA
                     IHNKIKDICISLKAEDYLNMPRKIDNKIEIHLDSKILKYYKELEKEKILELDRDIITA
                     SSAAVAANKLLQLANGAIYDNDKNVKELHREKLEALKEIIDVSNGKPIIVFYNYKHDY
                     NRLMKEFKSLKPRTIENSKDIYDWNNGRIQLLLCHPASTGHGLNLQSGGSIIVWFGLT
                     WSLELYQQANARLYRQGQRETVIIHHLICKGTIDEQVMEAIENKDKGQSALLEAVKAK
                     LKEYRE"
     gene            complement(18222..18548)
                     /locus_tag="KR440_000421"
     CDS             complement(18222..18548)
                     /locus_tag="KR440_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HBH0697489.1"
                     /translation="MLESKIEKRLKKEIELLGGKAMKFISPGEAGVPDRIVLLPEGHV
                     IFVELKAPGKKLRKLQQYKMRELRELGFKVKCVSTLKEIDDFIKEVKGWNLNHIHTKN
                     TQLEKL"
     gene            complement(18830..21247)
                     /locus_tag="KR440_000422"
     CDS             complement(18830..21247)
                     /locus_tag="KR440_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697490.1"
                     /translation="MDIKASEIEHINVRHDGQLMLAIGKNKLETHWKNKSILWSELVN
                     RLSKTLRTQETYTEYRKMSKTEKDRVKDVGGFVGGSLKNGRRKAENIANRSIITLDID
                     YANKDIWEDITLLNDYACLMYSTHSHTENNPRYRLVIPLTRPVLPEEYQAISRMIADT
                     IGIDMFDDTTYQPHRLMYFPSTSIDGDYIFKFQDGEFLNPNEILDLYLDWTDVSYWPE
                     SSRERQKFNTQLKKQQDPIEKAGIIGAFCRSYSIKETIENFLNEVYIPGIDETRYTYS
                     EGSTSGGVVIYDDKFSYSHHGTDPASGILCNAFDLVRIHKFGELDEDAKPETPVNRLP
                     SFTRMSEFASSDTKVRKTIGRENLDKAKDDFGDIDFEDDEWLTRLDYDNKGSYKKTTN
                     NILMFIENDPYLKGKIAYNEFSNRAVVLGKLPWRKDDKLNDWNDSDDSGLRHHIETIY
                     NISSPSKVNDALIIAFENNTFHPIKDYLNSLKWDGIKRVDTLLIDYLGAEDNHYTRTI
                     IRKVLVAAVARVFNPGIKFDNMMVLSGPQGMGKSTFIKKLGGDWYSDSLTTVQGKEAY
                     EQLQGVWLLEMGEMMATKKADIEAVKHFLSKSEDIYRVAYGKRTSRFLRQCVVIGTTN
                     DKEFLRDKTGNRRFWPIDTGVKKVNKSIFNGQLDNERNQIWAEAVELCKANEQLYLSD
                     EEKKEAERQQRTHSEENAKSGIIEEYLNKPITENWYDLSISEKREYIHGSDFGDLKEG
                     TILREKTCVMEIWVELFNGEPKQLTPILSREINDILKGLDDWISYNGNLRFGKIYGRQ
                     RAYVRKS"
     gene            complement(21259..23217)
                     /locus_tag="KR440_000423"
     CDS             complement(21259..23217)
                     /locus_tag="KR440_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HBH0697491.1"
                     /translation="MRTLSIDIETYSDLDIKKVGVYRYVDSANFEILLFAYAFDNEEV
                     KVIDLVNDEELPKEVIEALNDNKVIKSAFNANFERTAISKFLNINLKPNEWSCTMIKA
                     LTLGLPGSLDSVSKALKFNEDKQKMKEGKALIQYFCKPCKATKVNKGRTRNLPIHDME
                     KWNKFKEYCKQDVVVEREIRNKLSKYKTTEREIKLWYLDQRINDTGIKVDTELIENAI
                     ECDKRYTEKLTKEAIKITGLNNPNSPAQLKKWLSDKVGFEITSLTKESIPEILKQVDD
                     ENVVRILELRKLMSKTSIKKYEAMKLAKGNDNRVRGLLQFYGANRTGRWAGRLVQVQN
                     LPQNHIEDLDLARNLLKEGDFDLIELLYDSVPDVLSQLIRTAFIPSEGHRFIVSDFSA
                     IEARVIAWFAGEKWRLDVFNSHGKIYEASASHMFKVPIESIKKGDPLRQKGKISELAL
                     GYGGSIGALTSMGAIKMGLDEEELQPLVTTWRNANPNITKFWWDVDKAAKKAIKDRTI
                     VKLQHGIKFIYNPGVLFIELPSGRRLSYLRPKIEPHTTFSGDKITYEGMEQTSKQWKR
                     IDTYGPKLVENIVQATARDCLREAMFNVTDAGYSIVMHVHDELVIDVDKKGGSLEEVN
                     SIMGKEISWAKGLPLKADGYECDYYKKD"
     gene            complement(23217..23789)
                     /locus_tag="KR440_000424"
     CDS             complement(23217..23789)
                     /locus_tag="KR440_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HBH0697492.1"
                     /translation="MSNSVQSTKVVTGKVRLSYCNIFKSRAMVEGAEPKYSVCILIPK
                     SDKVTLGRIKKAIDAAKEQGKTSKWGGKLPGNLKTPLRDGDAERADEAEEYVGMYFLN
                     ANSTQKPGIVDKDLNEILDNTEVYSGCYGRVSINFFPYNSAGNKGIGCGLQNVQKLAD
                     GEVLGGARASAEADFSDDFEYEDEEEDFLS"
     gene            complement(23812..24384)
                     /locus_tag="KR440_000425"
     CDS             complement(23812..24384)
                     /locus_tag="KR440_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HBH0697493.1"
                     /translation="MSNQEITKLVENNLNLVHFSINRFFKSYVEQHPYLYEEFYQEGC
                     IGLYKAALNYDSNRGKFSTVAVSYIRIEMFKALRYGEKHYRNSIEGLDVSVFEDSKRP
                     LKDIHGFYEIQFNYDVDYIRKIARKSYLKDIDKIVDWILQDKKISDIGELLNVSSTVI
                     CGRIKLFSKQVRDLDKFGEITSNIKSIYQI"
     gene            complement(24437..24772)
                     /locus_tag="KR440_000426"
     CDS             complement(24437..24772)
                     /locus_tag="KR440_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697494.1"
                     /translation="MPLDAGIKDKLDESISIILDLKDRKKSIEKDLDYEKDNLLDMLK
                     RLNITEYNSTEENAKVIVMDFKRESLIKDKVMSTFLEVNDNHLDRVYIPEHIKVSPVC
                     FVSVRAKDN"
     gene            complement(24787..25953)
                     /locus_tag="KR440_000427"
     CDS             complement(24787..25953)
                     /locus_tag="KR440_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HBH0697495.1"
                     /translation="MPLQHARLSASGAHRWLHCTPSIKLEENYPPLTSIYAEEGTVAH
                     ELAEVKLMLEYEKISKKAYNARIKKIQKSEYYNSEMEDYIQSYVENVVELVNDSKAIC
                     DDVIVMLEERLDFSEWVPEGFGTGDVVVISDGILQVIDLKYGKGLEVSAIENPQLRLY
                     GLGAYNQFEMLYDIDLIKTTIIQPRLDNISSEEIEVTKLLTWADNVKKKAQMAFNGEG
                     EFVSGSHCGFCRAKNDCRKRAEDNLKLARKYDFADTFALNKYEIADILGFAKNIQDWL
                     KDVQSYALEQAEKHGVKYPGYKLVEGRSNRKYVDEQEVAKVLLNSDYDEEKIYKPRTL
                     KGISDMEKAIGKKSFAKLLSDLIIKPVGKATLVVESDKRSEINSIDSAKKDFEI"
     gene            complement(25965..26402)
                     /locus_tag="KR440_000428"
     CDS             complement(25965..26402)
                     /locus_tag="KR440_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697496.1"
                     /translation="MVEVNVNVKVKVEAPEFTNALLVVANALGGLNLGKAMQIEPINI
                     TDMKEEKKVEVKKAEKIKKVEVKEDVKEEIAEAKEEIEKNNENTTSEVKYTKEEVRTK
                     AAQVSKAGKKDKLKELFGEFGASKLSEVKEEDYSAFMNKLESL"
     gene            complement(26473..26622)
                     /locus_tag="KR440_000429"
     CDS             complement(26473..26622)
                     /locus_tag="KR440_000429"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697497.1"
                     /translation="MKLLSLDTNNKNVVTLKKDGKVIADIVFKDIKTGKKISVGILNK
                     KVLVK"
     gene            complement(26603..26995)
                     /locus_tag="KR440_000430"
     CDS             complement(26603..26995)
                     /locus_tag="KR440_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0697498.1"
                     /translation="MDISESIAKQFSDSLKSLIEIEISKQETDRVKSQTIEQKVKVLE
                     PKDIVVLIKRGYPNYLITVEEARGILKLDTAFMRRLVSTGLIKSLVRGDGRKISRYEV
                     DDFIERNQGKNLDELLKAVEWGDEIAES"
     gene            complement(27026..27223)
                     /locus_tag="KR440_000431"
     CDS             complement(27026..27223)
                     /locus_tag="KR440_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0697499.1"
                     /translation="MLIGDNIKQMLRKRDIKPYKLAKELNIDVSGLYKLLRNKSSNPT
                     IDTLIKLADYLDITLDELVGR"
     gene            27299..27673
                     /locus_tag="KR440_000432"
     CDS             27299..27673
                     /locus_tag="KR440_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697500.1"
                     /translation="MQFSNNISKELQESIRKTVSDTSNTEYFYYAEFQYKIILKSIEE
                     FEKELDDEHEIALKLTNFGKDVLMIVEEVGYHNPCLIHYYGIVNGVYSEILQHTSQIN
                     FMITSVKKTDPSKPARRIGFIL"
     gene            complement(27683..27892)
                     /locus_tag="KR440_000433"
     CDS             complement(27683..27892)
                     /locus_tag="KR440_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697501.1"
                     /translation="MENKKIEEIEKRITMLENQLQAQLFSQITAQSNSDFIDYMYQCI
                     VLYLHEMNKSEKRKISEITKERIRK"
     gene            complement(27939..28748)
                     /locus_tag="KR440_000434"
     CDS             complement(27939..28748)
                     /locus_tag="KR440_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBH0697502.1"
                     /translation="MNNLQIFKNKTFGEIRVIELNGEFWFVGKDIAEQLGYKDTSDAL
                     KRHVDDEDKGVGEIPTPGGNQNMKVINESGLYSLILSSKLPSAKLFKRWVTNEILPSI
                     RSTGTYNMIDLQTKLPTTYKEALQHLIEQVEVNEQLQLESKMKDQVIKELKPKADYTD
                     MILKNKGLVTITQIAKDYGMSGKEMNKILHERGIQYKQSGQWLLYKQHQGKGYTHSET
                     IDITRSNGMPDVKMTTKWTQKGRLFLYDLLKTNNILPDIEKGYSYQTSILG"
     gene            complement(28796..28999)
                     /locus_tag="KR440_000435"
     CDS             complement(28796..28999)
                     /locus_tag="KR440_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0697503.1"
                     /translation="MKKRSLKAGRIEAGYTQEELAYKIGIAKSTYNLKENGKRNFTEK
                     EMIMISYILNKTMDELFLEKRLT"
     gene            29176..29517
                     /locus_tag="KR440_000436"
     CDS             29176..29517
                     /locus_tag="KR440_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBH0697504.1"
                     /translation="MATFGERFKQLREEKKLTQDELVSKFNKVYFTSFNKSTISQYEN
                     NKRKPEINILENWADFFDVSIDYLLGRTLVRNHIDTVAAHRVNPHKDLPEEAQEQLND
                     YIEFLMNKYKK"
     gene            29529..30077
                     /locus_tag="KR440_000437"
     CDS             29529..30077
                     /locus_tag="KR440_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0697505.1"
                     /translation="MLILKKLRTEKGISLDKLSTDLNINKSTLSRIENGLREPKESFI
                     KDCSDYFGVSTDYLIGKINIDDSNKLTKCLNSTFPIRLKELRKKKELTQAELSKLLNC
                     SLSKIAMLETSKREPVKEDLLRISEFFNVSVDYLLGKTSIENYITTDEISKIIKSYES
                     LPKEAQEHINSYIEFLVDRYKK"
     gene            30153..30659
                     /locus_tag="KR440_000438"
     CDS             30153..30659
                     /locus_tag="KR440_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBH0697506.1"
                     /translation="MNKLDALLDLANNEEIEIYYTDKIADDIKGLYINRQGLKIISLL
                     NSLKQNNAKLIEILAEELGHHFTSVGNYVSSKNSYKNKILIDKTENKALKWACEFLIT
                     EEEIIHVINSHATSVYEIAEELQVSINFLLKRLEFLSKKKSMLDLGNNRFLVLTNLPN
                     FYIYEDIF"
     gene            31279..32148
                     /locus_tag="KR440_000439"
     CDS             31279..32148
                     /locus_tag="KR440_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HBH0697507.1"
                     /translation="MIKNFDEWKEYDGFSYGSGRSEKIWLINEKTKDIGLFKFPKTKE
                     TGEYWAEKLACEIASLLKIPCANVEIGTYNKRIGSMSYLINKNDEKLIEGVWLINKYY
                     PCYNTDKLYDYKNNEYYSLKMIFKSIEEYNIKNDFLKMLIFDYLIGNRDRHQNNWALL
                     ESQNKITLSPLYDNGSSLCCYVNIENIDSFFTDHQRFEALINTRSRSRIRIDGKNKKE
                     PTHLEVLKYLNSNYYEQTIDFVEKIINLMNEVNINTLIDNYSENIISPKLKKLLKIYL
                     KEKVLRIKNIYNL"
     gene            32161..32868
                     /locus_tag="KR440_000440"
     CDS             32161..32868
                     /locus_tag="KR440_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HBH0697508.1"
                     /translation="MSIKNGKDFLYVIWKDPKSRRQFIIGKLAKNGLYEFSYEYEIDE
                     AIKAGFNALISFDDISKTYYSEDLFSPFASRLPDKKRKDINKILDKYDMSEYDTYELL
                     KRSGARLPIDTLEFVDPIPLDIDKNIIRYFYIAGVRHYAGCEGHECDNSIELESNEAL
                     VLELEPENKFDPHAIKVFDKKENHIGYIPRYYSGELTSIINRGLKYNCIIKFVNKENN
                     CDECIKVKLEILFDEYN"
     gene            32947..34077
                     /locus_tag="KR440_000441"
     CDS             32947..34077
                     /locus_tag="KR440_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBH0697509.1"
                     /translation="MKGGVRKRSNKWYYYFDLGIVEGKRKKVERVGGNTKKEAEKALR
                     EALNEYENSGIVFEESNISLSDYLDFWYKEYVLLNCKYNTQESYRINIEKHIKPKLGA
                     YKVKALTPAILQNFINKKYKEDYSQNTLQVLKAILHRSLKSAVHPYKHIRENPMQYVS
                     IPKTKSKTETNKVKTITLEEFNQILNIFPQDSFQRIVLLIGFHTGMRRGEIIALKWDN
                     IDLDNKTITVKHTLIKKPNGMFELGQPKTESSCRTIFTGDTLIKALKEHKLYQKKMKL
                     KYGEFYFDSDWVCTKENGQQVNTHTLDTIVRQIRVALNNDFHFHSLRHAHATLLLENG
                     ANIKDIQNRLGHSQLSTTMDTYSHVTDKMKNETVDIFEKITN"
     gene            complement(34435..36123)
                     /gene="ilvB"
                     /locus_tag="KR440_000442"
     CDS             complement(34435..36123)
                     /gene="ilvB"
                     /locus_tag="KR440_000442"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HBH0697510.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPVIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKTFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWSAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKAGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSNMEELGKVLDVIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            complement(36176..37171)
                     /gene="ilvC"
                     /locus_tag="KR440_000443"
     CDS             complement(36176..37171)
                     /gene="ilvC"
                     /locus_tag="KR440_000443"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBH0697511.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VVIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDYT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDAAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
BASE COUNT        11672 a         6800 c         3931 g        14894 t
ORIGIN      
        1 taataaaaat ttttattttt tattttatat aaacaatatt ttttctaatt tgtggtataa
       61 taaaagcaag aagaactaca atctatttga cagtagagtg aagttcataa tttaaacaat
      121 tagtaattat ttaaatttgt ggaacttgat tttaaaatca aattcccagc cacttttact
      181 cttgccacga gttgagtggc tttttacttt tagaaatact ttacaaatta agcaaaatat
      241 taaactagca ataacgccag ctattacatt aagtaaaaag ttattcatac ttcccacctc
      301 ctttcattag gaagtaggtt ttatcccagt atgaactcca ctctataaat tgtagattac
      361 atcttcttgc taaaaatatt ataacatata attattacat attttaccta ttctatattt
      421 atttttttat tttgctatct tcttcgtccc ctctttctct ctctttcagc ttctttcatt
      481 gcttcctctt catcttctat ctttataagt attgaggcgg ctgctaacgc tctctcatta
      541 acttctaaat tcatatattc acttggcttc cactttaatt tttgaataca ataatgagtg
      601 atgctagcat caaaatcgcc acctctgatt agttttttgc ttcttctact ttatcctcaa
      661 aagatgtatc aaatccattg acttcattca cttttactgt ataattgaca tactcacctg
      721 ctgtaagcat tgtctttaat aactgagctt ctcccattac tccataacta ttttggagtt
      781 cggcatcctt taaatctgga aatactgtag atgctacaca taattcagct acataactat
      841 tgtagtcaat ttcacttgta tattgtccag tatgcttacc attgttacca atcactttta
      901 ctcttttagt acactttctt cttagtgctt cgtcttcttc agatgataaa acttttaatt
      961 cccattcaac tggtttccct tctttatcta aaaatctgtt actcgctaca tattttacat
     1021 tatcaacctt tattgcattt tgacttaaaa aagcacttaa attactcata ttattctaat
     1081 ctccttttat tttaattttt catataaaaa atacacattt ataacttata aatgtgtatt
     1141 ttactccata cctatatttt ttattacttc ttccaatgga atttttttag taactaattg
     1201 attataaaga aatagatact taacattcaa attttctgca cattgtttta aagtaatcat
     1261 ttcaccttta taattaactc taatactatt tcttttatta tttcgttgaa cttcccaagt
     1321 aacccatcta caatttgatg gttcgtaatt tccattcaca ttaattctat ctattgttaa
     1381 accttttcta tatccatttt ctatagacca gttataaaaa tttataaaat cctctagcca
     1441 ttcattgcat acttctatgc ctctacctcc ataataatta taatctttat tactcttatt
     1501 tgtgcacctt ttaatcatac ggtgataaac tctatataag ctatcattag atttattatg
     1561 cgttgtagag cgctctagta atatttcctt tcttatacag ccacaacttt gtgcttttcc
     1621 ttgttctaat gcacttgtac tagttattgt tctatttcca cattcacatt gacataacca
     1681 taaattgtga ctatatttat ctttcccaga cattttcaat actgtaagct tcccaaaagt
     1741 tttcccagtt atatctttaa atctcttatt ttgtcctttc cagtttggag aacaaccaca
     1801 atgtggaggc ttctcatttt taattgattg ctttaaatta ttttctactc tctcaacttt
     1861 attcccacac tcacattgac atagccagac ttttttacta tgtttatttc tccctaaata
     1921 tttaattaaa gtaagtttgc ctaatttaat tccactttca aattccatga aatacctcca
     1981 aataatttat acttacatca taatattttt caagtataat tcaattttat aacattccat
     2041 taagtaatgc aaaggattca actaattccc aatcctcaaa agtgaaatcc atatcttcat
     2101 ctaaatactc accatcagca tcaaatttag taattattcc actgtccata ttacaatctt
     2161 taagtactac tgtctgtctt cctacagcag atgtagggtc ttcatttgta acttgtatgt
     2221 caaaataaat atcctcacca gtttctttat atctgtaaag taattctcta aaaatagaag
     2281 tattataatg aaatgttgca cttccagtat ttgtactccc agttgtttta tttccctttg
     2341 ttgttcttcc tagaattgga acttcacttt tatttttttc cattttagcc tctaaatcta
     2401 tagcttgcat aaaattatat cttttacctt ctatagttat aaaacattca gctttctttg
     2461 cactaactgt atctttagca tttattgttt gagccatatt atcactctcc tctctaatta
     2521 actgaaacag tcatataaag cttactcata gcatttatta ccttaatagc atcagatact
     2581 atgacagttt tcttatcatt tccaagctct acactaacat catcagtttt aaaatcttct
     2641 attgccctta tattctctaa ttctttatgg tgtttaacaa catcattcca gaaacttatt
     2701 cttcctgcct tatcattcgg aactttacct aaatactttt cattaaataa agttgcaata
     2761 tcattagcaa tttggtcaag tactctaaca ctttggttac ttgaaaaatc gtcattttta
     2821 tcatctgtaa atgatacaaa agtatttata tcctctaaca catgaacttc atctcccacc
     2881 ttgtggaata taaatttacc agtttttaaa gcttcttcaa gttgtatttg tgtataatta
     2941 acatcaacat caaactcacc atcatacttt ttattagtat tagatttatt tatatcgcat
     3001 cctgctatag ctccagtagc ccaataaatc aagctagatt caactaaatc tttatcttta
     3061 atcttatttt ctatagacac tacaccttca taatctgcat cacttttctt atatagtact
     3121 gtttgaaact tagctcctac cttatctctc attctctttg taaattctac aaataaactt
     3181 ttaatttctg ttgttgtagc caaacaccct agtgcattaa atgaataact ttctatttta
     3241 tccaagaaag cttggtactc tgctcctgtc acagcttcgc cattggttcc accagtaaat
     3301 acaagtcctg cacttgcttc tagtgttgca tccttcttcc aagtgatata gtcattgtct
     3361 tgtaagtctg taataacctt tgctatttga gtatctacct tcttattatc taaaagtatt
     3421 acaacatcaa acttagcatt atcatctata tttgttgtaa ctgttacttt taagtcattt
     3481 cctcttatac cactatattt tgctgtggct atagtacaac tggctttaac gcctttattt
     3541 aatttataaa aatatcccaa ccttatattt ttgaataaat ctctcaaacc tttcagcttc
     3601 tcatgagtat aatcatatcc aaaatacttc actgaatact tctcaaaatc atcactggtt
     3661 acttggaatg cttcttcatc tatgccccaa tctaactcta aaggcattgc aacaatacct
     3721 ctatctgata atgaactggt tgccctctta gctgagataa aatttatata tgcaccagga
     3781 agtattttat tttgcgttac gaatgttcct ccacctaaag ccatctaact cactcctttc
     3841 ataaatttat ttattctatc ctctacctct gagaaggaat ataactcatt ttcttttaaa
     3901 attgcattta ataaatcttt tctatttaca tacttcttag agttaactat ctgctcctta
     3961 gtaaacttgt agttatcttc tttgcttaat gctttactca aaattatcac ctctcttcaa
     4021 accaccgaat aactctactg tatccatctt attggtatca ttatttttta tagtaaaata
     4081 gttataatca acaaagaagt gaagaacatt gtctataatt tcaaaattca tatttgtacc
     4141 tcttactaaa tctccatcaa tttctatata ctctaattcc tccagtagca tctcagctat
     4201 ctcatttatt tcaaaattct tatcttctga acgagggaaa taatgtacat caaaagaatt
     4261 tttctttaat tctctcccgc ttggatatgg tgtcttgctt ggatttaaag gaacaataaa
     4321 aaaacaaggt tcattaatac cttgctccac atcttcgcta taaattgtat atttttctcc
     4381 aaacgattta tctaacttta ctgatattcc atctataata ttattaagca tcaaacactc
     4441 ctttaagcaa tattaatagt tttttctcta ttatcttatc aacttgactt tgtagttcca
     4501 tttctgaaat agttaagaaa tgttgtcctt taacccaacc cttgccattt ttagttctat
     4561 gaccaaattc aacatatgca ttttgttatc ctaaaggctt tttatcctct agctcttata
     4621 gtttcctata agttcggcgt acatcatcaa caaaataaac tttatttagt tgcccagcac
     4681 tcttggagag attatattta ttcactctct acgctctacg gaaacctata gcctatccgc
     4741 aatctatagg ttttcctcgg tattggcata tataattaat taaatatttt tccatgttct
     4801 tctatggact atatttgata tagatgaata tgttacagga tatatttcac ttagttgttt
     4861 tattgtatat ccatcagaat acttttttct aatttctttt acatcctcaa tagataactt
     4921 agctctgctt tcttcaataa tcttaacttt ttctataagc ttatttccta ctaatccctt
     4981 agaatatcta cttcttaagc aagagtatga aatacctgtt ttttcagaaa gctctatcaa
     5041 tgtaatttct ttttcttcat gttttactaa tatatttgtt gacctatttc tacattgtgt
     5101 ctttatatcc acccatctac aattgcttga ttcataattt ccttcattat ttattctatc
     5161 aattgtaagt ttctctgaat atccattact caatgcccaa tttgcaaaat tatcatagca
     5221 aaaccattca tcacacacaa ttatattcct ttcaccatat ctattgtatc ttttattatt
     5281 tttatctgta catctagata ttataccctt ccatatactg taaagtcttt tattttgaac
     5341 cttatatttc tttttaaatt ggtatttgtc tgttaaattt aatttatctt gttctttttt
     5401 taaacatcca caagattgca ctaagccact agttaaagag tcagttctta tttctttgaa
     5461 atttccacaa tcacacttac ataaccaata tttcctattt cttttaccgc tttttatctc
     5521 tttagaaaat ttaatgactc ttagccttcc aaattttttt cctgtaatat ctaaaaattt
     5581 tgccataaaa tcacctcttt tatacattat aaatcaagag ttattctatg tccatattct
     5641 acctacttta taatgtaaca ctattacact tagcgtttac cgattttgct gggttttata
     5701 tgccccattg tgttaaggca tattcagttg gattaacaac ttctataata taattattac
     5761 cttgtttgta aacaggaagc gacctagcat aagctactcc actccaaccc tctctcaaaa
     5821 atcctgtatc aactggtgtc cttctaatta cttttcctaa gagtcttgct gctaattctc
     5881 ttgctgcatc cttgcaaaac ttatctaaat caatctttgt aaatttctcc atctttttac
     5941 aaactctttt aaactctcta aaatcaacac tgccccatct agccattatg ctttatcctt
     6001 aaataactca agtattattt cttgatgatt tggatatata gctgattctc cacttcttac
     6061 atactcttta tcatttataa taagttttga acctgcttta atttctatat ctggagatat
     6121 aaagagttta atagtttgct ctagcttagc taatttccct tctgtagcag aaactatatt
     6181 tttatatgaa agtttacatg gttgattttc taatacaatc acttctttat tgttagttcg
     6241 ttttgttaca gggtctttaa ttggctgata ctcaactata gtacatttat ctctatataa
     6301 catttctatt gcttttctag ttttacttac catcttaagc acctaaaggt taatatctta
     6361 ttcttaccat aagtagtaag ataagctatt aagctatcaa agcgttgttc tggtgtttga
     6421 gaaccacttc ctatagcaaa atctaccttt gtatcacctt ctgatataga cttttctaca
     6481 gcttcaaagt taatgctttc tatatctaat tgacccatat ttttcttggt aaataagaac
     6541 tctccaacta tcatatcaac ttcaatttct ttcaattcaa ttggcatagt ttttatatta
     6601 caatctagtt taataatatt ttctattttt tctcttacaa aacctattaa ccacttatct
     6661 ccatctttta atatatatcc aaaactttca agtctttttt ctatttcatc aattatatta
     6721 ttttccataa ttttcaccta ctttttagta agtttatttt tctctttaag ctgcttattt
     6781 tcttcttcta aagactcaac ttttgacctt aaaatattat tttcagctat taaatctttt
     6841 acacttaatg acttgccata ctttactgcc ttaccagttt catctatcaa atcatatccc
     6901 atctctaaga aatcatctat tttacattct tctatggtta gtattctatt tagttttttt
     6961 acttgtggca ctatatatca ctcctttttc tatgcttcaa caacaaattg tattgcatca
     7021 gcttttttat ttaatataaa tacatcctca aaactttctt caaagtagaa gtattttccc
     7081 tctgtaactg ctgttggttc gtctaactta gagaactgat aagaaacagg tgtaattatt
     7141 gcacttgggt gaactaagga cataaagatt tgtttagctc ctgctcctac tttccatcca
     7201 gttgtaaaat catatgcagt tttcattaga ttagatggta ctttaattat tttaactgtg
     7261 tcaatatctg tggtttgtct atttaaagaa gttccaccat cttttatgtt tactgttctt
     7321 tgtatctctt ttgcattttt gataagtgta tttactactg gagtaacata caatattctt
     7381 ccattttcag gtactctagc ttctgtcatt ttttccatta acttatcaaa tacttctaat
     7441 acgtttgctg ttgtaagaac agttgtatct gctgtgttac ctaatgcggt ccaatcagca
     7501 tatattttag atatacagta agcatccatc tctggaaact tttgttcctc attatatact
     7561 tttgttatat tacctattga agccacataa ttagtttggt ttatatctgc tggatgaacc
     7621 aatgttgacc atttcctttg attagttaat accttaggtt cccaagcatt atcatagttt
     7681 ctttgagcta ctgctattgt atctctgttt gaatctaccc ttccagttgt agatatagtt
     7741 ggtatttcta ttgttttaga acccgtccat ctatatcttc cattattttc tgttgcatac
     7801 aaatccccga agtttaaagt ataaggatat gcttgtgcta aaacatttga atattctttt
     7861 gcataattta gtgctgccat tttatttcct cctatttatt attattttca tgaggtctta
     7921 ccccagtaaa attaaaacca aaatcattta tcttaggctc ttgccctggt gttatagtat
     7981 ctattttagg ttcttcacct tctagtgttg cattaaacaa ataatcttta tcctgtttca
     8041 aagggtttat ttgctcttca aaagcttttt gtctatcttt actatttctt agggcttcca
     8101 tgtctaaatg agcttttaac gctatttcat ctctacattt aacagattta aaagcatcac
     8161 ctaaccagta attaaaatct ttttcttcaa tttctttttt gtaggtttct tccaaagttt
     8221 tcttatcagt ttcataagtt gtttttagat tctctacatc ttcttttgtc atacctcctt
     8281 caaacttttt aatagtttca ttagctgtat taagttgtgt ttcaagattt acataatctt
     8341 cttgagtaac tgtagtctcc tttattttct tttctataga tttttgaaga gaagctacat
     8401 caattttgtt atcctctact tttattcctt ctagcaattc ttttaaccaa tccattttaa
     8461 atttctcctt tcatttttta caaaataaaa gcacctacta atttattatt tagcaagtgc
     8521 ttttacatat ttactatttg tatatctttc cataaatcct ttagtaattt accatcaatg
     8581 ttgtaattat caaccatatc cttaccattt ttataatact ttgtatctcc attagggcaa
     8641 agagtaataa accttgtatc atcatctcca atagatatat tatatggttt attatataag
     8701 tcaaattcta tatctaatcc taaatcaata gaatcaatta aatgttgtaa attcttaaat
     8761 ttattatcca ttttattctc cattcaaaat atctttgtta gctattttat gagcttttgt
     8821 aagctccata tccttctctc tttttacctt atcatggtta ttttcatcag ctaaccaatc
     8881 atgtttatga ggtacaattt tatgttgctt tgggtttcta tggtcagtta agtctaaatc
     8941 taatctaggt tttcctgtat taccatagta tcttctttga attaatttac catctttgta
     9001 attatcaaat acactatttg gttttgattc aaatggcact gaatgaacac ttccactagt
     9061 taaatttctc tgattcttaa cttgccaatt tacatcctta taaagctttt tagcttcctc
     9121 ataccttata gtatcattat acttcatatg ttgatattca tcaaatttag aaggtatttc
     9181 atttcctaat acctttttat attcttcaaa ttgttttcta tctttactct catttagttg
     9241 catttttcta agggtgtcag ctttttcttt tccaagtcta ccctctatat gtttctcata
     9301 ccactcatta tacttcatac tagatggtac ataatatgtt tttccatctt ctcctcttgc
     9361 tgctctatag ccttcttcat cctcgaacca aggagctgtt gttgtcctac aacgacaatg
     9421 aaatggtgga gctgttattc caacttgata atctttcata tcaaatattt ttccatctaa
     9481 ctctctgcat atatttgaag ttcttaaatc taatgtagca ataatctcat atttctctac
     9541 atctaaatca ctaaaacaat cttttctact tgctgatgca aagaaagctg attcagtcat
     9601 tatcaaattc ttagcttgtg atttagatac attaaatctc ttagcaaagt catttactag
     9661 attttttgga ttttcacctc taataattga ttgagttagc ttagtatgta gttcatttat
     9721 taaagtaggt ctatgcttac cccaaattct ttcactaaag tttaatccat ctgttgccca
     9781 tggtttagag ataattttat ttattctatt agtatcaaga ctcattaaac tccaaccaac
     9841 gtttactcct tgttgaacat taaaagctgt atgatagtat ccacttgtat aaatatctct
     9901 cattagttta tcaataccat caagttcatt tccatataaa acttctacgt gttgctgtat
     9961 ttgtaacttt aaagcttcaa gccttgttat atgaagtctt gcactagcat tttctaactc
    10021 cttcatccac ttttgattta tagcattttc tttgccatat ttaatatact cttctacatt
    10081 ccacttaaat tcttctaatt ctcttgtatt taacagtttc ttagcttcta ataaagatat
    10141 tccttcattt tcagcaaatc tgttatacca tgataatata tctttttcta tactattcat
    10201 agctaatttg tatttctttt ccaattcaag ataatatttt acacttttat tgttttgaac
    10261 ttcttctaat tgttcaaatc ttttcctcca ataatcttta tgtttcatct ataacaccct
    10321 cttgattatt agtaggaatt aaatcatcat attctttttg ggtttcttcc tgttttttaa
    10381 gtctttcaag ttcgtcatta acatcttcaa cccaaggatg attactaaca atagtttcat
    10441 ctgatataat tccaattgat ttagaagcca tatctatttt ttcagactca tttattatca
    10501 tagaatgatt aaatgttatt tgaactgact tataatcata gctcttacta ccacttatct
    10561 ttaaatactc acacacaaac cataaaagct ctctaattgc ttttttaaac ttcttttcag
    10621 tcttggaaca ttttaaatca agtagtgaat ataaaaattt aagagctaca cccgatttgt
    10681 cacctgtgtt ttgagattcg ggattaactc cttgaccaaa aataattata ttcttttcta
    10741 atctatcaag aagctccttt ttggcttcaa ctggtatatt tatctctagt ttatcaactc
    10801 cacctccacc atctacttta attgatttat agtatcttat attatctata aactcttgta
    10861 gacttgttcc tggatactct tttaatacat aaatgacctc ttgcatttca tctaaattgt
    10921 ctgcaagtgt agaaatgtta ttatcatata tatcaattaa agacttatag aaagttaaat
    10981 ctgagacaca cttttcatta tttttaaaag gtataaatgg aactttaccc catccctgtt
    11041 ctttgttatt tattctaaaa tgaccttctt gtatatcagt catttttcca tattcatcat
    11101 ataaaaattc ttgaataaaa ctattacctc tttcaataaa gtaagttacg tcattttctg
    11161 tgtagtactc aactcttttt attttatttc catctatatc ttcaatataa taaaacctaa
    11221 taaatgcaac taattccctc tgtcttttac tatcccaaat aggaattgct tcttcagctg
    11281 gaattattac atatttaaac tcaccttttc tattaatata tggatgtaac cattcaaccc
    11341 ctttattact agcattgaga tatagttctg ttattgtatc gtcaaactct tctcctagta
    11401 agtcatttaa aagcttagtg agattatcat catctgcatt aaatactatg ggatttccga
    11461 cactatagcc taccttttgg tcaactaaaa gcttatggta gttgttaatt gctttattat
    11521 taactttagt aaaatcatca accttagctc catctaagag ataatatctt ctcttattgt
    11581 ttacatcagt attaccataa tagtattctt ctccttgttt atatttttct ggcctatgct
    11641 ttaagatgta gtgttctatg acttttacta ggttaaaggt gctctctttt tttaactgaa
    11701 cttttattaa atctgtttca cttatataaa tatttaacac ctcctttact ttaagaagct
    11761 tattccatta tttttaagct tattatctat agaatatcta agagcagcca ttgcatcatc
    11821 cataaactca actggttcat caagatataa tccagttctt tcgtcttgtt tccatttcca
    11881 ttgttgtatt tcttttatgg tattagtgca actaggatgt acatgtattc ttaattgttt
    11941 caaataatct atttgagctt taacacttcc tggtcctttt ttaactcctt tagctttata
    12001 tcctgcactc ttccacatct taattctatc tggttcagca ctatcacagt acataaatag
    12061 agtcttttct aaacctctac tatttgcaat ttttatgatt tctgaggtat ccatttcatg
    12121 tacatatatt tcattacata tatataactc tccatcctta aagccaattc taagtattgc
    12181 atttgcatga ttaaagccaa agtcttgtga cagtctcata ttgtcaaagt attcaaattc
    12241 tgtgggaaat tcatgtataa cataattttt aagtattgct ccaccagttt ctccccattc
    12301 tccaagacca tatactttgt acccttctgg gtcttgctct tttctcattt gcattcttct
    12361 gtagtaagct tcatctatga atctattttg tagataagta ctatgatgag taaatatatc
    12421 atcattttta tagtcaaaat actttctttt tatccaatga gtagctgaga ctggattaaa
    12481 tgtaaatgtc atttgataat acaagttagg attagttaaa atacctctta aacggtcatc
    12541 tagtatatct atgtcacttt ccataagttc tgtagcttct tcacaccaaa cccatgttaa
    12601 ttttcctttc gagaagttaa ttgattttaa tttttctctt tgttttgcat cattaactcc
    12661 tctgaaaatt atagagttac cagtaacttt actcttaatt tctaaaggat ttaaagtagt
    12721 tttccaatac ttatcagctt gtttaccata aatacgattt atagctcctg taagctctgc
    12781 atacgttgaa tacttatgtg tagcttctga ctttctaact actaatagat tagctccttg
    12841 atacttctta tctcctaact ttagtatata gtcttgtgct acattaacag attttccact
    12901 ccctgctgaa cctttcattg ctctgtatct ttttttagta aaattagctt ccttgaaatc
    12961 tggattaaaa tttattctaa ctatcatttc tatcaccata atctacactt attttcaact
    13021 catcatctcc aatatcatct ttatttaggt tagcaacctc acatttcaac ttctcaactc
    13081 ttgttttctg ctcctctgta gccaaattcc aatccttatg aatcatttca tcatactgct
    13141 taattaaact ccttaactca ctcatagctc tactctgtgc attaagaaaa gatgcttgcc
    13201 tatcccatgc aaattgaaat tcatactcta tcttctcact attttctgtg atttcatgtt
    13261 tctttaattc cttaatcatt tcctctttat ctttaacata cattatcttc tgtgctctta
    13321 ttattgctgc gtattgaatt gttatctgtt cccaaagaat atcaaattta tcttttatag
    13381 atatttcttg tattaattcc ctagtttctt caggtaagta ttttgagaag aaaccaaact
    13441 tttcagcatt tttattctct tttggagcac cataaccaac tgaattttta ttagaaaagg
    13501 gtgcacccct tttatttata ggtgcaccct tctttttttc actagaccaa ttgtatcttt
    13561 ttatccatga ctttaaagta tttaaactaa tgtcatactt tgctgatatt tccttttgtt
    13621 tcattccttt taagtaatct tgttttacct tttctttgac atcttgcaca tcaccacctc
    13681 tttatttgtt tgttttggga ataaaaaaac aatcctattt aataggattg tttcaaatct
    13741 agtctcctct atccttccag gataaatgaa acatacttat tattaatgta gttagagata
    13801 aaattatatg aactgtaaaa aatattaaaa catagaaaat tatattagaa taatatcgag
    13861 taagcattat tgtattgata aaacatatta caacaaccaa aatctccatt attacagtat
    13921 aatatgcttt taactgtatg aattgaaagt atgatatatt aaatccattt acctttcttt
    13981 ctgtttttat atcttttgct tctgatattg atttgttata tgtagtaaac aataatgtaa
    14041 taatgcaaac tccaaaagct actaatagtc ctgacaaagt tagtacatct ttgtttaaag
    14101 aaattacaac ttctttatta attgacgaat taaaaaacat gtatgaaata atacatatta
    14161 atactggtgt taaaactaac tctactactt ctttagaatt tatagccttg taataattga
    14221 gtaagatatt taataccttt ttatacattt taacacctcc ttagcaatct atgcttcact
    14281 catagcaact tcttgataca ttaaattaaa aaaaactgaa ttattctcaa ttaaatcatc
    14341 tatcaaatta ttcatcttct caaaaatgtc ttcactatct atatgattat ctatatcaag
    14401 agaaacctct atttctctac acaactttat accctcagta tccaacttta attttctctt
    14461 atttttctct ccctcaatta ttatcctatt tattttttta tttctaaatt cctctccttt
    14521 aatatatttt tcgcaacatc ttttaacttc actaggtaaa tatctagttc taaatgtggg
    14581 cttatatatc aaatcgttat gtcgtcttga attatttaaa ttagaaaaca aattatcttc
    14641 atcagttcca aacttctctc tatcaacatt taaagttatt tttgaaacac catctaaatc
    14701 taatatttgt tctataaaat ctttacttgc tatttgtgta atattaattg atgtttttga
    14761 catattatta tcaaatttat tattttcaat catatcctca taatatagat gtaagttttt
    14821 acttagttca atcatgggaa cccccacaaa aactctttca aataatacta ctgctatatt
    14881 attttccttc ataaatttaa taaataggtg ctgcttattg ctatctcctt gatccatttt
    14941 tttattaccc tctacttctt tagtatgagc atttataata tttgtattct gattgtattt
    15001 agtatattca agtttcacct tgattattaa atcatcctcg ctacatttta cttctgtaat
    15061 ccaaaacttt ttatctttag taatattaat tccatttttg ttttctttac aatattcttt
    15121 atccttaaag aaggtcttta ttacctcttt taaaatttct tcatctgaat agacactccc
    15181 attaatttta tttataatat ctattctata aacatcaata tatctttttt taaaattact
    15241 cattatacca ttattccttt cacaaatatt tataatgata taattctata taatcagtca
    15301 atatccttca ataatcattc gacaaccaca aaataagtct aaataatact tttaccttat
    15361 tcctgtctat aatgtacaaa tatatttcat ttttaattca ttacataaaa aaaaagaccc
    15421 tctattaata cagtcattta aatcatttcg attaattctt taatcttttt atatacctct
    15481 ttataattca tacctttatc tatcaaccta ggtaatttca tagatataac tctttcaagt
    15541 acttgtatta ataaaaaaac tcttgtattt catctgtaaa atcttttctg accttttacc
    15601 ttctgttctt atatccagcc attcttgatc tttttctgtg gcaatatagt aaccatgtat
    15661 tctaatttgt ttaagtgctt ttgtaaccca cttcgtaaat agttttgctt ctgctttatt
    15721 acttctaaat gacatattgt acacagcttc ttctgtaaca aaagtagtac caaagttagg
    15781 caatttatct ttaaagtttc tagtgtagga atctccgaca gtagactcat taaatttctt
    15841 tttatattct ctatctatat ttcttaatgt atcacgaata tttactatgc ctagttcctc
    15901 tcctacgtca tttgcattaa accaaacttc ttctccattt tttgaccata tcatccttac
    15961 atttttctct tgtaaaattt tcaacatact actacctcct aaattagtta ttaataaaga
    16021 atattcaatt tgaacaccct tattttcgac ccttcaatat gagagattaa aattgtataa
    16081 aaaaagacct agaaatcact ctaagtcatg aattactcat ttatcattct ccttatttac
    16141 aaaataaaaa agactaagtt ggggtaactt agcctttttc aagggggaat atattataca
    16201 cttgtttcat actaccatta taacaggggt taaatatcat taaaatatca tctttttatc
    16261 atgtatttat caaacagtta atttcaagcc atctatacca aataaataca ctccaagttc
    16321 ttttaccatt tcattaatcc aacgtcttga tgtaactact ccacaattta atatctctgc
    16381 tatttcttca tatgtttttt catcacaata gtaaagctct aaagctctat acttttcata
    16441 agaatgtaaa cgctcttgtc ttaatttcaa agttttcatt gctgaatcta tatgtgctat
    16501 cataataata gttcttgctt tacttttctt tatacttaaa atatacaact cttcattatc
    16561 aagcttacct aaatcatcct ctaagtaatc tacatcttta gcttcactta ccgctgagtc
    16621 tatatgtctc ttaaaatcat tataatgctt catcaataat ctagtattat ataaaacgcc
    16681 ttttttccta tcttctcttt cttcttttct taactctctt acaatttctt ttatactttc
    16741 tttatccact taaatcactc cttcttgctc tcttcgaaca acttctactg caatactcaa
    16801 ctttagattt atccaactta taaaatcttc taccacacca agcacatctt actattttac
    16861 ctgggcttat aagttccatc ttttcttttt actttcatac cttcactccc tatattcttt
    16921 taattttgct tttactgctt caagtaatgc actttgccct ttatccttat tctctatagc
    16981 ttccattact tgttcatcta ttgtaccttt acagattaga tgatggataa taacagtttc
    17041 cctttgaccc tgtctataaa gccttgcatt agcttgttga tacagttcta agctccaagt
    17101 taatccaaac caaacaatta tacttccacc tgattgtaaa ttaagtccat gtcctgtact
    17161 tgctggatga caaagtaata attgtattct tccattattc caatcatata tatcttttga
    17221 gttttctatt gttcttggtt tcaaactctt aaactctttc attaaacgat tataatcatg
    17281 tttatagtta taaaaaacta taataggctt accatttgaa acatctataa tttcttttaa
    17341 agcttctaat ttttctctat ggagttcctt tacattttta tcattatcat aaattgctcc
    17401 attagctaat tgtaaaagtt tatttgctgc tacagctgct gatgaagctg ttattatatc
    17461 tctatctaat tctaatatct tctctttttc taattctttg taatatttaa gtattttact
    17521 atcaaggtgt atttcaattt tattatctat ttttctaggc atattcaaat aatcttctgc
    17581 ttttaagctt atacaaatat cttttatttt attatgtatt gcattttctg ctccattttt
    17641 aggttgccaa ttatatatag ctcctgtttg ataatttttt tgtcctggct caaagtatcg
    17701 ttctttatat cctgtaatag ttttacctag tctttcacct ctatcaagta agtacatctg
    17761 tgcccataag tctattaaac tatttggtgt tggtgtacca gtaagcccaa ctactctttt
    17821 agttaaaggc aatactttct ttaaactttt aaatcgttgt gctttatttg atttaaaaga
    17881 actaagttca tctatcacaa ccatatcaaa tggccatttt cttttataaa aatccactat
    17941 ccagggtacc atttctctat ttattatata aatatctgag tctgtactta aagctctcac
    18001 tctatctaat ttactaccaa gaactttaga tacttttaga tgctttagat gttcccattt
    18061 ttttacttca ctactccagg tatctcttgc aactcttaaa ggtgctataa ctaaaacttt
    18121 tgatatatca aagtaatcat acattaattc acttatagct gttagagtac agactgtttt
    18181 acctaatccc atatctaata gtaaacctat attattatta tctatagttt ttctaattgt
    18241 gtattcttgg tatggatgtg gtttaaattc catcccttaa cctccttgat aaaatcatct
    18301 atctctttta atgtgcttac acacttaact ttaaatccta attctcttaa ttctcgcatt
    18361 ttatattgtt gaagcttcct tagtttttta cctggtgctt taagttctac aaatataaca
    18421 tgtccttctg gtaataagac aatcctatct ggcacacctg cctcacctgg tgaaataaac
    18481 ttcatagcct ttccacctaa caactcaatc tcttttttaa gtcttttttc tatttttgat
    18541 tctaacaaaa tatcacttcc ttaaaattta agtgtcaaca atgtcaacag attttctata
    18601 tatatgtata tatacatatt aggcatacat atgtatatac gtatatgcct aatattactt
    18661 atactacttt atacttaatt tttgttgaca ttgttgacat taatacctca aacattgtaa
    18721 ttactgcatc ttaggtgtca acataagcgt caacaaagcc attttttttg ttgacattgt
    18781 tgacactttt aaaattttca aatttactct gtttttaatt ctgttgacac taacttttac
    18841 gtacataagc tctctgccta ccatatattt tcccaaacct taaatttcca ttatatgata
    18901 tccaatcatc taatcctttt aatatatcat taatttctct tgataagata ggcgtgagtt
    18961 gtttaggttc tccattaaat agctcaaccc atatctccat aacacacgtt ttttctctta
    19021 atattgttcc ttcttttaaa tcaccaaaat ctgaaccatg aatatattct ctcttttctg
    19081 aaatacttaa atcataccaa ttctcagtaa taggtttatt tagatactct tcgataattc
    19141 cagatttagc attttcttct gagtgagttc tttgttgtct ttcagcttct tttttctcct
    19201 catctgatag atatagctgt tcattggctt tacataattc tactgcttct gcccaaattt
    19261 gatttctttc attatcaagt tgaccattaa atatactctt gttaaccttc tttactcctg
    19321 tatctattgg ccaaaacctt ctattcccgg tcttatctct taaaaattct ttatcattag
    19381 ttgttcctat aactacacat tgacgtaaaa accttgatgt tctcttccca tatgcgaccc
    19441 tatatatatc ctctgatttg cttagaaaat gcttaactgc ctcaatatct gcttttttag
    19501 tagccatcat ttcacccatt tccagcaacc atactccttg taattgttca tacgcttctt
    19561 taccttgtac agtagttaaa ctatcagaat accagtctcc accaagtttt ttaataaaag
    19621 tactttttcc cattccttga ggaccagata aaaccatcat attatcaaac tttattcctg
    19681 gattaaatac tcttgctact gctgctacta aaacttttct tattatagtc cttgtatagt
    19741 ggttatcttc tgcacctaaa tagtcaatta aaagtgtatc cactctctta atgccatccc
    19801 actttaaaga atttaaataa tcttttatag gatgaaaagt attattttca aaagcaatta
    19861 ttagagcatc atttactttt gatggtgatg agatattgta aattgtttct atatgatgtc
    19921 taagcccaga atcatcacta tcattccaat catttaattt atcatctttt ctccaaggta
    19981 acttacctaa aacgacagct ctatttgaaa attcattata agctattttt cctttcaaat
    20041 atgggtcatt ttctataaac attaagatat tgtttgttgt tttcttataa cttcccttat
    20101 tgtcataatc taacctagtt aaccattcat catcttcaaa atctatatca ccaaaatcat
    20161 ccttagcttt atcaaggttt tctcttccta tagtctttcg tactttagtg tcactgcttg
    20221 caaattcgct cattcgagta aatgaaggta atctatttac aggcgtttcg ggtttagcat
    20281 cttcatcaag ttcaccaaat ttatgtatcc taactaaatc aaaagcattg cataaaattc
    20341 cgcttgctgg gtctgtacca tgatgactat atgaaaactt gtcatcataa ataactactc
    20401 caccacttgt actaccttct gaatatgtat acctggtttc atcaatacca ggaatatata
    20461 cttcatttaa gaaattttct atagtttctt ttatactata ggacctgcaa aatgcaccta
    20521 taattccagc cttttcaata gggtcttgtt gtttttttaa ttgtgtatta aacttttgcc
    20581 tctccctcga actttctggc caatagctta catctgtcca gtctaaatat aaatctagta
    20641 tttcatttgg gtttaaaaac tctccatctt gaaatttaaa tatatagtct ccatcaattg
    20701 aagtacttgg aaaatacata agtctatgag gttgatatgt agtatcatca aacatatcta
    20761 ttcctatagt atctgctatc atcctggaaa ttgcttgata ttcttctggt agtactggtc
    20821 tagttaaagg tattacaagt ctatatctgg gattattctc agtatgtgag tgcgtagaat
    20881 acataagaca agcataatca tttaataatg ttatatcctc ccatatatct ttattcgcat
    20941 aatctatatc taaagttata atacttctat ttgcaatatt ctctgctttt cgtcttccat
    21001 tttttaagct tccacctaca aatccaccaa catctttaac tctatctttt tcagtctttg
    21061 acatcttcct atattctgta tatgtttctt gagttcttaa tgttttactt aatctattta
    21121 caagttcgga ccataaaata cttttatttt tccaatgtgt ttctaattta ttttttccta
    21181 tggccagcat gagttggcca tcatgtctta catttatgtg ttcaatttca ctggccttta
    21241 tatccataaa tcaatcacct agtctttctt ataataatca cattcatatc catctgcttt
    21301 aagaggaaga cctttagccc aagatatttc ttttcccata atactgttaa cttcttctaa
    21361 agaacctcct tttttatcta catcaattac aagctcatca tgtacatgca ttacaatgct
    21421 ataacctgca tctgtcacat taaacatagc ctctcttaag caatctctag ctgtagcttg
    21481 aacaatattc tcaactaact taggtccata agtatctatt cttttccatt gtttacttgt
    21541 ctgttccata ccttcatatg ttatcttatc cccactaaat gtagtatgag gctctatctt
    21601 aggtcttaga tatgataatc ttctaccact tggtagttct ataaataaaa ctcctggatt
    21661 ataaataaat ttaatcccat gttggagttt tactatagtc ctatctttta tggctttttt
    21721 agctgcctta tctacatccc accaaaattt agttatattt ggattagcat ttctccatgt
    21781 tgttacaaga ggttgaagct cctcttcatc aagacccatt tttatagccc ccattgatgt
    21841 taaagctcct atgcttcccc catatccaag agctagctct gaaatcttac ctttttgtct
    21901 aagtgggtca ccttttttaa tactttctat tggaacttta aacatatgac tagcactagc
    21961 ttcatatatt ttcccgtgag aattaaatac atctaatctc cacttctcac cagcgaacca
    22021 agctataacc ctagcttcta ttgcactaaa atctgatact ataaatctat gaccttcact
    22081 tggtataaaa gctgtcctta tcaattgact taagacatct ggtacactat catataaaag
    22141 ctctattaaa tcaaaatctc cttcttttaa taggtttcta gctaggtcta aatcctctat
    22201 atgattttgt ggtaaattct gtacttgtac taatcttcct gcccatctac cagtcctatt
    22261 agccccataa aactgtagta gacctcttac tctattgtca ttacctttag ctaatttcat
    22321 agcctcatat ttctttatag aagttttgga cattaatttt ctaagttcta aaattctaac
    22381 tacattttca tcatcaactt gttttaatat ttctggaata ctttcttttg ttaggcttgt
    22441 aatctcaaag ccaactttat cacttaacca tttttttaat tgagctggac tatttggatt
    22501 atttagacca gttattttaa ttgcttcttt tgtaagtttt tcagtatatc ttttatcaca
    22561 ttctattgca ttctctatta actctgtatc aactttaata ccagtatcat taattctctg
    22621 gtctaaatac cataatttaa tttctctttc ggtagtctta tacttactaa gtttgtttct
    22681 tatttctctt tcaactacaa catcttgttt acaatattct ttaaatttat tccatttttc
    22741 catatcatgt attggtaaat ttctagttct tcccttgtta acttttgtag ccttacaagg
    22801 tttacagaaa tattgtatta atgctttacc ttctttcatt ttctgtttat cttcattaaa
    22861 ctttaaagcc ttagacacac tatctaaact tcctggaagt cctagtgtta acgcctttat
    22921 cattgtacat gaccactcat ttggttttaa attaatattt aaaaatttac ttattgctgt
    22981 tctttcaaaa ttagcattaa atgctgattt tataacttta ttatcattta aagcttctat
    23041 tacttctttt ggtaactctt catcatttac taaatcaata acttttacct cttcattatc
    23101 aaaagcatag gcaaataaca gtatctcaaa attagcagag tctacatatc tgtagactcc
    23161 aacttttttt atatctaaat cactatatgt ttctatatca attgataagg tcctcattaa
    23221 cttaagaagt cctcttcttc atcttcatat tcaaagtcat ctgagaaatc tgcttctgca
    23281 ctagctctag ctcctccaag tacttctcca tctgctaact tttgaacatt ttgtaagcca
    23341 caacctattc ctttatttcc tgcactatta tatgggaaaa agtttatact aactcttcca
    23401 taacaaccac tatatacctc tgtattatct aatatttcat ttaagtcttt atcaactatt
    23461 cctggctttt gtgtactatt tgcatttaaa aaatacattc ctacatattc ctctgcttca
    23521 tcagctcttt ctgcatctcc atcacgaaga ggtgttttta aattacctgg taattttcca
    23581 ccccatttag aagttttacc ttgttcttta gctgcatcaa tagccttctt tattcttcct
    23641 aaagtcacct tatctgattt tggtattaaa atacaaactg aatacttggg ctctgcacct
    23701 tctaccattg ctctgctttt aaagatattg caataactta atcttacctt tcctgttact
    23761 acctttgttg attgtactga attactcata attttcttcc tcctaatatt tttatatttg
    23821 gtatattgat tttatatttg aagttatttc tccaaattta tctaaatctc taacttgctt
    23881 actaaacaat ttaattcttc cacaaataac tgttgaagaa acatttaata actctcctat
    23941 atcagatatc tttttatctt gtaaaatcca atctactatt ttatcaatat cttttaaata
    24001 tgactttctt gcaatttttc ttatataatc aacatcatag ttaaattgaa tttcataaaa
    24061 tccatgtata tcttttaatg gtcttttact atcttcaaat acacttacat ctaaaccttc
    24121 tatagagttt ctataatgtt tttcgccata acgcaaagct ttaaacattt ctattcttat
    24181 gtaagaaaca gccactgtag aaaacttgcc tctgttacta tcataattta atgctgcttt
    24241 atataaccca atacatcctt cttgataaaa ctcttcatat aagtatggat gttgctctac
    24301 ataagactta aaaaatctat ttatactaaa atgtacaaga ttcaaattat tttcaactag
    24361 tttagttatt tcttgattac tcataaaatt atatcccctt ttaaatcttt ttatagctac
    24421 taggaattaa atatttttaa ttatcctttg ctcttactga tacaaagcat actggactaa
    24481 cttttatatg ttctggtata tacactctat caagatgatt gtcatttact tcgaggaaag
    24541 ttgacatcac tttatccttt attaaacttt ctcttttaaa atccatcact ataacttttg
    24601 cattttcttc agttgagtta tattctgtaa tatttagtct ctttaacata tctagtaaat
    24661 tatctttttc ataatctaaa tctttttcga tactcttttt tctatccttt aaatctaaaa
    24721 ttatacttat gctttcatct aatttatctt ttatacctgc atctaatggc atgtaatatc
    24781 ccccatttat atttcaaaat cttttttcgc tgaatctata ctattaattt ctgacctttt
    24841 atcactttct actactaaag tagcttttcc aactggtttt ataattaaat cacttagtag
    24901 tttagcaaaa ctctttttac ctatagcttt ctccatatca ctaattcctt ttaaggtcct
    24961 tggcttataa attttttctt catcataatc tgaatttaat aaaaccttag caacttcttg
    25021 ctcatctaca tactttctat tacttctgcc ttctacaagc ttatatcctg gatatttaac
    25081 accatgcttt tcagcttgtt ctagtgcata actttgaaca tcctttaacc aatcttgtat
    25141 attcttagca aaacctaaaa tatctgctat ctcatattta ttaagagcaa atgtgtcagc
    25201 aaaatcatat tttctagcta gtttaaggtt atcctcagct ctttttctgc aatcattttt
    25261 agctctacaa aatccacaat gactgccact tacaaactct ccttcaccat taaaagccat
    25321 ttgagccttc ttcttaacat tatctgccca tgtaagtaat ttagtaactt ctatttcttc
    25381 acttgatatg ttatcaagtc taggttgaat tattgttgtt ttaattaaat caatatcgta
    25441 tagcatttca aactgattat atgcccctaa accatatagt ctaagttgag gattctctat
    25501 agctgaaact tctaaacctt ttccatactt taggtctata acttgaagta taccatcaga
    25561 tattacaaca acgtctcctg ttccaaatcc ttctggaacc cactcactaa aatcgagtct
    25621 ttcttctaac atcactatta catcatcaca tatagcttta ctatcattta ctaattcaac
    25681 tacattctca acataagatt gtatatagtc ctccatttca gagttataat actcactttt
    25741 ctgtatcttt ttaattcttg cattatatgc ctttttactt attttttcat actctagcat
    25801 taatttaact tctgctaatt catgtgcaac tgttccttct tctgcatata tactagttaa
    25861 tggtggatag ttttcttcta attttatact aggagtgcag tgaagccatc tatgggctcc
    25921 actcgcacta agtcttgcat gttgtaatgg catttatgta ccccctataa actttctaat
    25981 ttgttcataa aagctgaata atcttcttct tttacttcac ttaacttact agctccaaat
    26041 tcaccaaata actccttaag cttatccttc ttacctgcct tacttacttg tgctgcctta
    26101 gttcttacct cttcttttgt gtatttaact tcactagttg tattttcatt attcttttct
    26161 atttcttcct ttgcttctgc tatttcttct ttaacgtctt cttttacttc taccttttta
    26221 atcttttcag cttttttaac ttcaactttc ttctcttctt tcatatctgt tatatttata
    26281 ggttctatct gcattgcttt tcctagatta agacctccta aagcattcgc cactactaat
    26341 agtgcatttg taaattctgg tgcttctact ttaactttta catttacatt aacttcaacc
    26401 attttaaata tctccttttt gtgttatact ttacttgtgt tatattttat tttttatttt
    26461 tttgtgtgtt ggttatttta ccaacacttt tttatttaat attccaaccg atattttctt
    26521 accagtttta atatctttaa atactatatc tgctataact tttccatctt ttttaagagt
    26581 tactacattc ttattattag tatcaagact cagcaatttc atccccccac tctactgctt
    26641 ttaaaagttc atccaaattt ttaccttgat ttctttcaat aaaatcatca acttcatatc
    26701 ttgaaatttt tctaccatca cctctaacta gtgattttat caaacctgtg cttactaacc
    26761 tacgcataaa agctgtatct aactttaaaa ttccccttgc ttcttctact gttattagat
    26821 aatttggata acctcttttt attaaaacaa ctatatcttt aggctccagt acctttacct
    26881 tttgttcaat agtttgactc ttaactctat ctgtctcttg tttacttatc tctatttcta
    26941 ttaaactttt taaactatca ctaaattgtt ttgctatact ctcagaaata tccatgtgaa
    27001 aattctctcc cctcttttta attatttatc ttccaactaa ttcgtctaat gtaatatcta
    27061 aataatcagc taattttatt agagtatcta tagttggatt agaactttta tttctcagta
    27121 atttatataa accacttaca tctatattca attcttttgc caatttataa ggttttatat
    27181 ctctttttct tagcatttgc tttatattgt cgcctattaa cattgtattc acctcagttc
    27241 tacatggtat aatataaaca tggaataaat tccaaaattt tcaaaaggat ggtgtattat
    27301 gcagttttca aacaatatat ctaaagaatt acaagaaagt ataagaaaga cagtctctga
    27361 tacttctaac acagagtatt tttactatgc tgaatttcaa tataaaatta ttcttaaatc
    27421 tatagaggaa tttgaaaaag aacttgatga tgaacatgaa attgcactaa aattgactaa
    27481 ctttggaaaa gatgtcttaa tgattgttga agaagtaggt tatcataacc cttgtctaat
    27541 tcactactac ggcatagtaa atggagttta ttctgaaatt cttcaacaca catcacagat
    27601 aaactttatg ataacttcag taaagaaaac tgacccttca aaacctgcta gaagaatagg
    27661 ttttatattg taattatcat tcttattttc tgattctttc tttagtaatt tcactaattt
    27721 ttcttttttc acttttattc atctcatgta aatacaaaac tatacattgg tacatataat
    27781 ctataaaatc actatttgat tgagctgtta tctgactaaa taactgtgct tgcaattgat
    27841 tttcaagcat ggttattctt ttctctattt cttctatttt cttattttcc aattcaatct
    27901 ctcctttcta aaatattctg tatttagttt tcaaagtact aacctaatat tgaagtttga
    27961 taactatatc ctttttctat atctggtaat atgttatttg ttttcaataa atcatataaa
    28021 aacaatcttc ccttttgagt ccacttagtt gtcattttta catcaggcat tccattactt
    28081 ctagttatgt ctattgtttc tgaatgagtg tatcccttcc cttgatgttg tttatataaa
    28141 agccattgtc cactttgttt atattgaatc cctctttcat gaagtatttt attcatttct
    28201 tttccactca ttccatagtc ttttgctatt tgagttattg tgacaagtcc tttatttttt
    28261 aatatcatat ctgtataatc tgcctttggt tttagttctt ttattacttg gtctttcatt
    28321 ttactttcta gttgtaactg ctcatttact tctacttgct ctataagatg ttgtaacgct
    28381 tctttatatg tagttggtag tttagtttgc aaatctatca tattatatgt cccagtacta
    28441 cgtatacttg gtaatatctc attagttacc caacgtttaa ataattttgc acttggcaac
    28501 ttagaactta atattaaaga ataaagacca ctttcattaa ttactttcat attttgatta
    28561 ccaccaggag tcggtatttc acctactcct ttatcttcat catcaacatg tcttttcaaa
    28621 gcatcagatg tatctttata tcctaattgt tctgcaatat cttttccaac aaaccaaaat
    28681 tctccattta gttcgattac tcttatttct ccaaaagtct tatttttgaa tatttgtaaa
    28741 ttattcataa tttctttcct ccttatgatt gtttttgtga acaataaatt ttaatttatg
    28801 ttaacctttt ttctaaaaaa agttcatcca ttgttttatt taaaatatag ctaatcatta
    28861 tcatttcttt ttccgtaaaa tttctttttc cattttcttt tagattatat gttgattttg
    28921 ctattcctat cttatatgct agttcttctt gggtatatcc tgcttctatt cgaccagctt
    28981 ttaaacttct tttcttcatg ttcttcacct tccaatgttt tatttttgtg aacttcatac
    29041 ttttattata ttcacacaaa caaaacttgt caatacataa gtttattttt ttgttaactt
    29101 ttttattttt ttctaaacat aagtttacgt ttctgtaaac ttatagtatc atttaattat
    29161 tgggaggtgt tgtttgtggc aacttttgga gaacgtttca aacaattacg agaagagaaa
    29221 aaacttactc aagatgaact agtttcaaaa ttcaataaag tttactttac aagttttaat
    29281 aaatcaacaa tatcgcaata tgaaaataat aaaagaaaac cagaaataaa tattctagaa
    29341 aattgggcag atttttttga tgtatcaatt gattatcttt tgggtagaac tcttgttaga
    29401 aatcacatcg atactgtagc cgcacacaga gttaatcctc acaaagattt accagaggaa
    29461 gctcaagaac aactcaacga ttatattgaa tttttaatga ataaatataa aaaataagga
    29521 ttgaatctat gttaatatta aaaaaattga gaactgaaaa aggaatttct ttagataaat
    29581 taagcacaga cttaaatata aataaatcca ctttatccag aattgaaaat gggttaagag
    29641 aaccaaaaga atcatttatt aaagattgct cagactattt tggagtatct acagattact
    29701 taattggaaa aattaacata gatgattcaa ataaattaac aaaatgctta aattcaactt
    29761 ttccaataag gttaaaagaa ttaagaaaga aaaaagaatt aactcaagca gaattatcaa
    29821 aattacttaa ttgttcacta agcaaaattg ctatgttaga aactagcaaa agagaacctg
    29881 ttaaagaaga cttgttaaga atttcagaat tttttaatgt gtcagttgat tatctacttg
    29941 gtaaaacctc aattgaaaat tacataacta ctgatgaaat atccaaaata attaaatctt
    30001 atgaaagttt acctaaagaa gcgcaagaac atattaatag ctacatagag tttttagttg
    30061 atagatataa aaaatgaata tttagagcag ttcactcctg ctctttatat ataaaaaagt
    30121 aacacataca ttctttttat agggggattt caatgaacaa actagacgca cttttagact
    30181 tagcaaataa tgaagagata gaaatttact acactgacaa aatagcagat gacataaaag
    30241 gattgtatat aaacagacaa ggactaaaaa ttatatcatt acttaattca ttaaaacaaa
    30301 acaatgctaa actaatagaa atcttagcag aagaattagg acatcatttt accagtgttg
    30361 gaaactatgt atcttcaaaa aacagttaca aaaataaaat cttgatagac aaaactgaaa
    30421 acaaagcact aaaatgggca tgtgaatttc ttataacaga agaagaaata atacatgtta
    30481 ttaattcaca cgctacaagt gtatacgaaa tagctgaaga attacaagtt agcatcaact
    30541 tcttactaaa aagattagaa tttctatcaa aaaagaaaag catgttggac ttaggaaata
    30601 atagattttt agtattaact aatttgccaa atttctacat atatgaggat attttttaaa
    30661 ctcatttatt ctacttttat agatttttta cttaataaat atatatttca atattattat
    30721 aataaactac acataaaagc taaaaaattg taagaatatt aagaaaataa ttaagtaaaa
    30781 accagataaa caaaattaag ataatattgt acataacaaa agtatataaa gagcagttaa
    30841 tctgctcttt tatataaaca ccaaacatac atacattcta aaagggagag atactattat
    30901 gaaaggtgga gtaagaaaaa gaagcaacaa atggtattac tactttgacc taggcatagt
    30961 atctgaataa agcaatatca gcttaccttt tggtacaaaa aatatttctt acttaacaac
    31021 actcaaaaaa attaccaaat aaatatagaa aactaaaatt aagtatttat aaagtaaaac
    31081 cattatacac agtagtaatt ttgatgagta aacacatatt ccatatgtaa taaagcttga
    31141 agttaaaagt ggaataaata aaatctagaa aaaattttta ttttaagtta agcttattat
    31201 ggataataaa attcttgcaa atttagaatc aaatgataca ataagaatat aaaatacttg
    31261 tttgagagga ttattttcgt gattaaaaat tttgatgaat ggaaagagta tgatggtttt
    31321 tcttatggta gtggaagaag cgagaaaatc tggctaatta atgaaaaaac taaagatata
    31381 ggcttgttta aattccctaa aacaaaagaa actggagaat attgggcaga aaaattagcc
    31441 tgtgaaatag ctagtttgtt aaagattcct tgtgctaatg ttgagatagg tacatataat
    31501 aaaagaattg gttcaatgag ttatttaatc aataaaaatg atgagaaatt gattgaaggc
    31561 gtgtggttaa taaacaaata ttatccttgt tataatacag ataagttata tgattataaa
    31621 aataatgaat actattcatt gaaaatgatt tttaaatcta tagaagaata taacataaaa
    31681 aatgattttc taaaaatgtt aatttttgat tacttgattg gaaatagaga taggcatcaa
    31741 aataattggg ctctactaga aagtcaaaat aaaattactc ttagtcctct atatgataac
    31801 ggatcatcat tatgttgtta tgttaatatt gagaacatag attctttctt cactgatcat
    31861 caaagatttg aagctcttat aaatacacgt tcaagatcaa gaataaggat agatggaaaa
    31921 aataaaaaag aaccaacaca cttagaagtg ctaaaatatt taaattcaaa ttattatgaa
    31981 caaactatag attttgtaga aaagataata aatcttatga atgaagtaaa cataaataca
    32041 ttaatagata actattcaga aaatattata agcccaaagt taaaaaaatt gctaaaaata
    32101 tatctaaaag aaaaagtgtt aagaataaaa aatatatata atttgtgaaa gagggataaa
    32161 atgtcaatta aaaatggaaa agatttttta tatgttattt ggaaagatcc aaaaagtaga
    32221 agacaattta taattggaaa gttagctaaa aatggattgt atgaattttc ttatgaatat
    32281 gaaattgatg aagctattaa agcaggtttt aatgctttaa tatcatttga tgatattagt
    32341 aaaacatatt atagtgaaga tttatttagc ccatttgcaa gtagattacc tgataaaaaa
    32401 agaaaggaca tcaataaaat tttggataag tatgatatgt ctgagtatga tacatatgaa
    32461 cttttaaaaa gaagtggtgc aagattacct atagatacac tagaatttgt agatccaatt
    32521 cctttggaca ttgataaaaa tataattaga tatttttata ttgcaggagt aagacattat
    32581 gctgggtgtg aaggacatga atgtgataat tcaatagaat tagaatcaaa tgaagctcta
    32641 gttctagagt tagaaccaga aaataaattt gatcctcatg caatcaaggt atttgataaa
    32701 aaagagaatc atataggtta cattcctaga tattatagtg gagaattaac aagtataata
    32761 aatagaggat taaagtataa ttgcattatt aaatttgtta acaaagaaaa taattgtgat
    32821 gaatgtataa aagttaaatt agaaatatta tttgacgaat ataattaatg tcttcttgta
    32881 aatataggag acatttttta tataaacacc aaacaaacat acattctaaa agggagggat
    32941 actattatga aaggtggagt aagaaaaaga agcaacaaat ggtattacta ctttgaccta
    33001 ggcatagtag aaggaaaaag aaaaaaagta gaaagagttg gtggcaatac aaaaaaagaa
    33061 gcagaaaaag cactaagaga agcactaaat gaatatgaaa actctggcat agtctttgaa
    33121 gaaagtaaca tcagcttatc agactaccta gacttttggt acaaagaata tgttttactc
    33181 aactgtaaat acaacactca agaaagctac cgaataaaca tagaaaaaca tataaagcca
    33241 aaactaggag cttacaaagt aaaagcttta actcctgcaa tactacaaaa cttcataaat
    33301 aaaaagtaca aagaggatta ttctcaaaat acattacaag tattaaaagc catattacat
    33361 aggtcattaa aatcagcagt ccatccttac aaacacatac gagaaaaccc tatgcaatat
    33421 gtaagcatac caaaaactaa atctaaaaca gaaactaata aagttaaaac tattacatta
    33481 gaagaattta atcaaatact aaatatattt cctcaagatt catttcaacg tatagtttta
    33541 ctaattggat ttcatactgg tatgcgaaga ggtgaaatta ttgcactaaa atgggataat
    33601 atagaccttg ataataaaac tatcacagta aagcatactt tgattaaaaa accaaatgga
    33661 atgtttgaat tagggcaacc aaaaacagaa agctcttgca gaactatatt tacaggtgac
    33721 actttaataa aggcattaaa agaacataaa ttatatcaaa agaaaatgaa attaaaatac
    33781 ggagaatttt actttgatag tgattgggta tgtaccaaag aaaatggtca acaagtgaat
    33841 actcacactt tagacactat agtaagacaa attcgagtag ctttaaacaa tgacttccat
    33901 tttcattctt taagacatgc acatgctact ctattattag aaaatggtgc taacattaaa
    33961 gacatacaaa accgtttggg tcatagccaa ttatcaacta caatggatac ctattcacat
    34021 gtaactgata aaatgaaaaa tgaaactgta gatatatttg aaaaaattac aaattagagt
    34081 ttgccaccca aaaatataat acggtggcaa atgggtggca aaatctaatt tatctatttt
    34141 aaaagctaaa attatcaaat ttatatagtc agctatacgc ttgtaatttc aaggctttag
    34201 agtatataac aaccataact aatataaggt attaataata aatctaacga ataaaactta
    34261 atatttactt aagttgtaat actattctaa attgtataat taaaatttaa taagttctta
    34321 attatatatt cattgattta tttgcaaaaa taaaaaatgc caatctatct ctaaacaaaa
    34381 actgaatacc aataaaaatt taataaaaaa ttttatcaat attcagtata agttttagtt
    34441 acatatcaaa ttttccaatg catcattagg tgctacaata ggataaacat cataatcctt
    34501 atctatatca cattgtaaaa atacaggttt attaaaatct atcacatcta gaacctttcc
    34561 taattcttcc atattggata ccttatatcc atcaatatta tatgcattta cgagtttaac
    34621 ataatccaca ttttcattaa tatcagtttg agagtatctt tgattggaaa ataacttctg
    34681 ccattgtctt accattccta atgttctatt attgagtagt aaaataagca taggtacatt
    34741 ataatttgca actgtagcta gttcattaca gttcattcta aaacttccat caccagttac
    34801 taaaaccaca tttttatcta cattaccagc tttagttcca atagctgcac ctaaaccaaa
    34861 tcccatagtt cctaaccctg ctgatgttat aaaactttta ttacctttaa aattccagta
    34921 tttagcagac cacatttgat gttgaccgac atcagttact accacagtgg gtttctttaa
    34981 tgtttcatat ttttcattta tttttttaag tatattttgt ggatgaaatt catatgtttg
    35041 tacaccctca ctctttctaa atgtctttat ctcttctttc caattgctat tattcttact
    35101 ttcaaccctt tcaattaata aactaaggac tagctttaca tctccaacca aagatacatt
    35161 agattctata tttttactta tttcagatgg gtctatgtct atgtgtatta tttttgcatt
    35221 tttagcaaat tcagaacttt tacttatcac tctatcacta aatctcgcac ctatcgctat
    35281 tactaaatct gaattagaaa gtgctaaatt actttctcta cttccatgca ttccaaccat
    35341 tccaagagat agttcattct ttctatctat atttccaagc cccataagtg tatttagtac
    35401 gggtgtatct atcttagttg caaatttttc aagaatttcc tcactgtctg aagatttaac
    35461 acctccacct gcatatatta caggcttttt agattctttt atgatatcta ttgcttcatt
    35521 taaaagctta atattagttt catcatctag atcaaaatca ctcttataat ccatataatc
    35581 atctatttga cataaatcat agtcctcacc actaaaatcc atttctgcta aaaataaatc
    35641 ttttggaaca tctacaagta caggtccttt tctacctgaa tttgcaactc taaaagcttc
    35701 ttttattgta ggaactaatt ctttagtgtt tctaactaaa taattatgtt ttgtcataga
    35761 taatgttgct ccagttatgt caatttcttg aaaagaatct tttcctaata aactagttgg
    35821 cacctgccct gaaattacaa ccataggtga agaatccata aaagctgttg ctattccagt
    35881 tatggcattt gttgctcctg gaccagatgt tgtaaaacaa actcctactg tattagtact
    35941 tcttgcgtac ccatctgcag catgaactaa accttgttca tgggatgtcc ttatatgttt
    36001 aaaatcatct gaataatcgt atagagcatc ataaagaggt atcacggctc caccaggata
    36061 tccaaatatt gtgtcaattc cctctttctt taaacattct aaaattacct ttgctccatt
    36121 catacgcact actatacacc actctttccc tcaaactaat ccatttaata tatatttatt
    36181 ttaagaatga catcatactt cttagatttc taccaacttc ggcaatttct gtattatatt
    36241 cttcttctct agtttttagg aagttttcac gtccttcttc attttcttta atccacgctt
    36301 ttgcaaattt tccattttgt atatcctcta aaacattttg cataccttgt tttgcagcat
    36361 cagttattac ccttttacca gatacataat ctccatattc tgctgtgtca cttatactat
    36421 atctcattct ttcaaatcca ccttcatata taaggtcgac tattagtttc atttcatgta
    36481 aacattcaaa gtatgctact tctttttgat aacctgcatc tacaagcgtt ttatatccca
    36541 attttataag ttcacttatt ccaccacaaa gtactgattg ttctccaaat aaatctgttt
    36601 ctgtttcttc tttaaaagta gtttctaaaa cacctgctct tgttgctcct atcccttttg
    36661 catatgctaa aactgtctct gtagcttttt tagtgtagtc ttgatatact gcaaatagtg
    36721 caggaactcc acttcccttt gtaaatacat ttctaactaa gtgaccaggt cctttaggag
    36781 ctaccattac aacatcaaca aattctggtg gttgtacttg attataatgt atattgaatc
    36841 catgagcaaa agctaatgtc tgaccttctt ttaagttatc ctttacactc tcttcataaa
    36901 ctttcttttg tttttcatca ggcattaaca tcattgtcaa atcactttct tttgtagctt
    36961 ctgctacact cttaacttca aatccatctt cttttgcttt ttgtgctgat ttacttccgt
    37021 cataaagacc tatcactaca tgtacaccgt tatctctaag attttgtgca tgtgcatgtc
    37081 cttgacttcc ataacctaaa actgcaacct ttttattttt taaaacctct aaatccacat
    37141 ctttttcata atacattctt gccattttca tttcctccca atactttttc ttatttttat
    37201 gatatttatt tttattagaa attcatactc attatcattg ttttaatatt attcaacaga
    37261 tttaaattct aaatttatat aatcttacaa ttataat
//
LOCUS       DAEPOZ010000010        35996 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.010, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000010 DAEPOZ010000000
VERSION     DAEPOZ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35996
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.010"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            194..1585
                     /locus_tag="KR440_000444"
     CDS             194..1585
                     /locus_tag="KR440_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBH0697512.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
     gene            1838..3631
                     /locus_tag="KR440_000445"
     CDS             1838..3631
                     /locus_tag="KR440_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBH0697513.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDGIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILDELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIVPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(3712..5094)
                     /locus_tag="KR440_000446"
     CDS             complement(3712..5094)
                     /locus_tag="KR440_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBH0697514.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPSNGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            5352..5699
                     /locus_tag="KR440_000447"
     CDS             5352..5699
                     /locus_tag="KR440_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBH0697515.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            5717..6751
                     /locus_tag="KR440_000448"
     CDS             5717..6751
                     /locus_tag="KR440_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697516.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVVWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(6800..7849)
                     /locus_tag="KR440_000449"
     CDS             complement(6800..7849)
                     /locus_tag="KR440_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0697517.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENRVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            8124..9659
                     /locus_tag="KR440_000450"
     CDS             8124..9659
                     /locus_tag="KR440_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697518.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFNLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(9845..10531)
                     /locus_tag="KR440_000451"
     CDS             complement(9845..10531)
                     /locus_tag="KR440_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HBH0697519.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEGVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            10656..12038
                     /locus_tag="KR440_000452"
     CDS             10656..12038
                     /locus_tag="KR440_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBH0697520.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
     gene            12440..13708
                     /gene="eam"
                     /locus_tag="KR440_000453"
     CDS             12440..13708
                     /gene="eam"
                     /locus_tag="KR440_000453"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HBH0697521.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDICDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(13801..14157)
                     /locus_tag="KR440_000454"
     CDS             complement(13801..14157)
                     /locus_tag="KR440_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697522.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(14308..15402)
                     /locus_tag="KR440_000455"
     CDS             complement(14308..15402)
                     /locus_tag="KR440_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBH0697523.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
     gene            15866..16687
                     /locus_tag="KR440_000456"
     CDS             15866..16687
                     /locus_tag="KR440_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBH0697524.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
     gene            16708..17904
                     /locus_tag="KR440_000457"
     CDS             16708..17904
                     /locus_tag="KR440_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBH0697525.1"
                     /translation="MFIEFFYLLRSRGLNISLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSINYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMLSR"
     gene            18070..21468
                     /locus_tag="KR440_000458"
     CDS             18070..21468
                     /locus_tag="KR440_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBH0697526.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNTLLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVVTVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIISFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSSINQDLETEDIGYRSINKSFKDEENRYKFIDGYNIQI
                     HNDLENEIYISKNASRQSGILSGIDVVHTPEFEEELAGLGMSQNFFKISDSLGVLSIN
                     NTDYSSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICSGLGNVNSEYAGIAEDSELIIIKLGKIDGFYNS
                     AMLFAASQYAYKKAFELRRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPHVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITVGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            21478..23151
                     /locus_tag="KR440_000459"
     CDS             21478..23151
                     /locus_tag="KR440_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBH0697527.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPVDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSQLNIAINRND
                     GSLSQDNIGTGTLVSGILAGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     ISGEYDIYLPNKNLIAPDTRFLDPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            23425..23619
                     /locus_tag="KR440_000460"
     CDS             23425..23619
                     /locus_tag="KR440_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HBH0697528.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     misc_binding    23788..24021
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            24098..25495
                     /gene="asnS"
                     /locus_tag="KR440_000461"
     CDS             24098..25495
                     /gene="asnS"
                     /locus_tag="KR440_000461"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBH0697529.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     gene            complement(25668..26843)
                     /locus_tag="KR440_000462"
     CDS             complement(25668..26843)
                     /locus_tag="KR440_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH0697530.1"
                     /translation="MNLEKIKKFFGFPLDKKHKENVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFATPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCDETDAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIEKAITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            27262..28626
                     /locus_tag="KR440_000463"
     CDS             27262..28626
                     /locus_tag="KR440_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBH0697531.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLINPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            28648..29139
                     /locus_tag="KR440_000464"
     CDS             28648..29139
                     /locus_tag="KR440_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBH0697532.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            29412..30083
                     /locus_tag="KR440_000465"
     CDS             29412..30083
                     /locus_tag="KR440_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBH0697533.1"
                     /translation="MLDKVKGRLIVSCQALENEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            30168..31034
                     /locus_tag="KR440_000466"
     CDS             30168..31034
                     /locus_tag="KR440_000466"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBH0697534.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            31067..32569
                     /locus_tag="KR440_000467"
     CDS             31067..32569
                     /locus_tag="KR440_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBH0697535.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSVVLAGLTGIPVNVLIVV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFIGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            32699..33151
                     /locus_tag="KR440_000468"
     CDS             32699..33151
                     /locus_tag="KR440_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBH0697536.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            33311..34114
                     /locus_tag="KR440_000469"
     CDS             33311..34114
                     /locus_tag="KR440_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697537.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDALYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACNECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            34172..35059
                     /locus_tag="KR440_000470"
     CDS             34172..35059
                     /locus_tag="KR440_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBH0697538.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEVWLGAGRGATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNINGSSFQELASTSSLIKKVAKIKNLNEKDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            35078..35926
                     /locus_tag="KR440_000471"
     CDS             35078..35926
                     /locus_tag="KR440_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH0697539.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
BASE COUNT        14027 a         4102 c         6152 g        11715 t
ORIGIN      
        1 attaagattt tctactgaag atactaaaaa aataatattt ttcataattt taaaaataat
       61 aataaaaaaa tgaaaataca ggcgataatc tattgagtaa acatagaaaa gatgatatta
      121 taagtatgtg ctaaaatggt taaagcaata ttaatattaa attcaactat aaaacaaaag
      181 ggggcgtttt tttatgtcaa gtacagaaag cgtatctaaa aaaagtggtc atccgaagag
      241 tttttggctt atttgcatga caattttgtg ggaaagattt agttattatg gtttaacagc
      301 tttattggta ttatttttta cagcaagtgt agctcaaggt ggtttaggtc tttctactca
      361 agaagcaaca tccatgtttg gcttatttac aggtcttata tatcttacac cattagtcgg
      421 tggctggtta gcagataaag ttttaggtca acaaaaatgt atttctatag gtgcactttt
      481 aatagcaata ggagatttcg tactatttgc ttcatctaca tctattgcca tggtttgggt
      541 aggtctatca ataattattt taggaaatgg tttttttaaa tcaagttgtt caaatatagt
      601 tggggatata tatcctaaaa ctgatgtgtc aagaaaagat gcagcataca gtttattcta
      661 tatggcagta aatgtaggag catttatagc tccaataata tgtggattgc tttctgataa
      721 tgtatttgca gtaagaggag caagtggaga tatagtatct tatggatata agatggcatt
      781 tttaatttgt ggtataggca tgatgttagg tatgattata tttacacttc ttgcaccaaa
      841 attattaaat gaagtcggaa aatatcctgt gactaaaggt aataaaggtg aaaaaattga
      901 gtatggacca cttactaagt tggagaaaaa taggataata gcaatggtta ttttattttt
      961 atttgtggtc ttattctgga cagcatacta tcaaacacaa agttctttta tgatttatgt
     1021 aagggataat gtaaatagaa ctctatttgg atttgaaatg ccagtagcat ggcttacatc
     1081 actaaatgct atattatgtg tggctttatg cccagtatta gctaccctat gggttaaatt
     1141 atctcatacc aaaagaggtg atttctctat tccagttaaa atgggattag gaatgataat
     1201 aatgggtgtt gggtttatag ttatggtatt tgctacatta gctaatggtc cagatggaat
     1261 ggtaaaggct agtatattgt ttatagtatt gacttatata tttacaacag taggtgaatt
     1321 attcttatct cctataggac ttgcaatgtt caataaatta gctccagcta agtatgctac
     1381 attatctatg ggagcctggt atctaacaag tttttttgca agtttattat caggaaagat
     1441 tgctggtttt acagccacac ttggtttttt acagatattt ggaggaatag caacagtttt
     1501 aataatattt ggtttaatat tattgggaat aagaaatgta ttaacaagat taatggctat
     1561 ggatttacta acaaaagaaa aataattcat gacgaattat tgtaaataga tgaattactt
     1621 caaataaagg aagttacatt taaatataaa attaaatgta atttcctctt tgattatgaa
     1681 aaaaagcttt acacaaatag atattataat taaaattata attgtgataa ttagagtgta
     1741 taagatttat ataaattata caaaaaatat ttatgtaata aactttacat acttatatat
     1801 ttagttattc aaactattat ttcgaggagg tcttgttgtg aatataaaag atagattaag
     1861 tggattaaga aaatttatgg aagaaaaaaa tatagatgcc tatatgattc catcttctga
     1921 taatcaccaa agtgaatatg taggagatta cttcaaatca agagaattta tatcagggtt
     1981 taatggttca gcagggacgg ttattgtgac taaagacgaa gctgggttat ggaccgatgg
     2041 aagatatttt atacaggcag aatctcaatt agaaggaagt acaataaaat tattcaaaat
     2101 gggacaagaa ggttgcccaa ctacagatga atatttatat aaaaatattc ctgaaggagg
     2161 aactttagga tttgatggta gagttatatc tgctagagaa ggagctacat tagctgaaaa
     2221 attatctaaa aaaggtataa agatagaata tcagtatgat ttaatagatg gtatatggcc
     2281 agatagacct gctttatcag atagtaaagc atttttatta gatgttaaat attgtggaga
     2341 aagttttagc tcaaaattag ctagattaag agaaaaaatg agtgaaaaag gtacttctac
     2401 tcatgtaata actactcttg atgatatagc atggttattc aatataagag gtggagatgt
     2461 taaatataat ccagttgtac tttcttatgc agtaataact ttaaaagaag tttatttatt
     2521 tgttgatgaa agtaagttaa atgaagaaat attagatgaa ctagctaagg aaaatgttca
     2581 aataaagcca tataatgatg tgtatgaatt tgtaaaaaat atagacaaaa ctgaaaaagt
     2641 tttattagat ggaactaagc ttagttatac aatttacaat aatatacctt gtgaagttga
     2701 gaaagttgat gagttcaatc cagtaatgtt ttttaaagct caaaaaaatg aggtagaatt
     2761 agaaaatata agaaattcac atgtaaaaga tggtgtggct ttcactaaat ttatgtactg
     2821 gttaaagaaa aatgtaggta agatggaaat cactgaaata agtgctactc aaaaattaga
     2881 ggacttaaga agagagcaag aaggtttctt tgagccaagt ttcaatacaa ttgctgcata
     2941 taaagagcat gctgcaatga tgcactatag tgcaactcca gagtcaaatt ataaattaga
     3001 ggcagaagga ttatttttag tagattcagg tggacaatat tatgatggaa ctactgatat
     3061 aactaggact acagttttag gacctataag tgatgaatta aaactacact ttacttctgt
     3121 agcaagagga atgataaatc tttctaaggc taagttcttg catggttgta gaggatataa
     3181 cttagacata ctatctagaa gttgcatgtg gaatatggga attgattatc aatgtggaac
     3241 tggacatggt ataggatttg tgctaaatgt tcacgaagct ccaaatggat ttagatggag
     3301 agtagtacct gaaagatttg atagtgcagt tcttgaagaa ggaatggtaa ctactaatga
     3361 accaggtata tatatagaag gttctcatgg tataagaaca gagaatgaaa ttgtagttag
     3421 aaaagctgaa aagaatttct atggtcaatt tatggaattt gaagttgtta cgttagctcc
     3481 tattgactta gatggtatag taccagaatt aatgaataag gatgaaaaag attatttaaa
     3541 ctggtatcat aagttagttt atgataaaat atcacctttc ttaactgatg aagaaagaga
     3601 atggttaaaa gtttacacta gagctatata gtttgtacta gatacaataa aaatcgaggt
     3661 attataatgc ctcgattttt tattgtatat tttattaact ttaaaaaatt tttaatttga
     3721 taattctact aattttttat tcattataag tagtataatt ccaaatatca ataaaaatga
     3781 tggtatcact atgaagactt gtaacatacc aagttggtct ataatagctt gcgtatagcc
     3841 agataattta tttgctgcaa atttgcctaa aaaccaaaca cccattagga atgaaatata
     3901 cttttcaggc gaaagtttat ttaccatacc atacccgact ggagaaaagc aaatttctcc
     3961 tatactttgg aatgttaaaa atccaactac ccaaaataca cttgctttgt gaattgaatt
     4021 agggtctgtt cctgtttgta taactgctac tatcataaac aaaaatgcta tagctaatag
     4081 tataaaacct aagctcattt tttttggcac acttaaatcg ccttttttag tttgagataa
     4141 ttttacccat actaaagcag agataggtcc aagaataaca caaaggagac cattgtatga
     4201 atctatccat gaagtaggaa tttgaaaatt gccaacagta agatttatgt gttcctttgt
     4261 atagagtgca atagacatag aggcttggtt atatgctatc caaaaaaata ctgtgaaaaa
     4321 agctaacacc aatattacta ttattctttt tttctcaatc aaagttaaag gtcttgaagc
     4381 gagcattttc ttatattcta gtgattttct tatagactct ctcccgttac tagggtcatt
     4441 attcaaaggt ttataaaact ttctgtttcc aaatataaag aagaataaac ctattagaac
     4501 cgtaattgat gaaaataaaa atacatatct atatccatac atacttatag ataatccgct
     4561 tttcatagca aaccattcat tagcaactaa accgcatatt aaagggccaa ataaagtacc
     4621 tagattagta gccatatacg taatagagaa agctccatct tttcttgggt catcattggt
     4681 atataagttt ccaaccatag tatgtaagtt tcctttataa aaacctattc ctaatccaga
     4741 taaaaatatc atgatgttta tatccatctt actatttgct atgtatccta gaaaataacc
     4801 aattgaaacc attagcattc caagtataat acagtttttg ggacctaaaa atctatcact
     4861 gacccatcca ccaataactg gagagaaata agtccatgct aaaaaagatg caagcattga
     4921 tgcggattct gctggtgtta accctaaacc acctttggag acttgttcag ctaaaaacaa
     4981 taataatacc aatttcatac catagtatgc aagtcctaca aaggtaaata ttgaacagca
     5041 gaaatataaa tctctaggtt gtttaagttt ttcttgcgtt tttaaaactg acatatgtcg
     5101 agaacctcca gataaatagt ttaacaaaat tttaccatac tatatgagta tatgtttttt
     5161 tcatatagac gtcaacttgt aagataattt aattaagtcg aaatatatgt aaaaatagta
     5221 tcatttttgt gtattcagta atattttttt atgataatat atgttattat tatattaaaa
     5281 tacaaacata tttatggaaa ggatatgcca atttagtagt tagattgatt gtttggcagg
     5341 tgggatgagc tatgataaga aagaaatctg aattacaagt agaagtaaaa gaaaatttaa
     5401 atggtggtat aggaaaggtt aagcttgaaa acataataca aaatgaagag cttaaaggaa
     5461 aaggaagact atttaaaaga gtagctttac cagtaggaag ttcaattggt gtgcatgacc
     5521 atacaacaga ttttgaagta tattatatac taaagggaaa aggtaaagta tttgataatg
     5581 gggaatttgt agaagtaaat gagggtgatg tagtgtatac agctgatggc gaaaaacatt
     5641 ctatagaaaa tataggagaa gaagatcttg agtttgtagc actagtttta tatatgtaaa
     5701 atatggagga ggagtcatga caggcaagat agaagaaatt aaggacaaga taaatagatt
     5761 ttgcaactcc aatttaaatc aagaatacaa agatataagc tttaaaattt tgcaaaacct
     5821 tttagataat aataaagaga taattcattc ttctagagct gatatatggt cttcagctat
     5881 actaaatata gttttagaac aaaatctttt atacaataag aagcatccac tacatataac
     5941 aaaaaaagag ttttcaaaac aaataggtgt ttctttaaat actataaata ataaatcgtc
     6001 tttaataaaa gatgtttgta gtattgactt tttaaatcaa gatactatag ctatttctaa
     6061 tgtagaattt tgggtaaaag agaccaattc caaatcaaag tctgtagatt tagatttaga
     6121 aaagaaaaaa atactatata aaagatatat taagctatca caagatgcga atagtgatat
     6181 tgaatcaatt agatatatgg aggaagctgt aaatataggt aaaagtatga ttactaaaga
     6241 agataaagaa cttggatttt ggaaaggttt atctaccaga gcctatatga tagcacttga
     6301 atcccttgct agaaagttag aaagtattaa taatctaaag gagtccaaaa aagtattaga
     6361 atatttaata gaaattaatc ctagtgatga gcagggaata agatacaaat tattaaatgt
     6421 attaattaga ttaaatgata gaacttcaat aaataaatta tttgaacttt ataaagaaga
     6481 aaaatctgct acatggatgt acagtaaagc tctttattac tttaaaaata aaagtatgtt
     6541 tttggcaaat gattctatta aggttgctag aagtaaaaat aagtacgttg gattatattt
     6601 gttagattgg cgaaatgctt ttggtagaga atttgttaca gaggaagaaa aggcagaagc
     6661 tgtctattac tatgatgaaa acatagtggt ttggaatgaa gttaaaggtt ctatggattg
     6721 gctattaaaa aaaatgttgg aattttcata aaaaaaacta ctccttactt ttatataagt
     6781 aaaaggagta gtttttaatt tatacatcta gttttaaatc ttcaggtttt attagacctt
     6841 tttttctcat aaattctgaa atcaacaaag ttacaatagc aggaagtata aaattaagta
     6901 aaagtatacc aacccacata gatatacctc cttttccagc actttgcata gcagttactg
     6961 ttccaaattg tccaaccaat ccacaagtac ccataccaga accaatagga atattctcca
     7021 ttttgaatat tgttgtagat aatggtccaa gtaatattga agctacagta ggtggtatcc
     7081 aaattttcca attttttatt atattaggta cttgcaacat agatgtccca agaccttgtg
     7141 ccacaaggcc accaactcta ttttctctaa agctcataac agcaaagcct accatttggc
     7201 aacaacaacc tacagttgct gcaccaccag caagaccacc taaacttaac atcatacaaa
     7261 tagctgcact acttataggt aatgtaagag ctattccaac aagtgcagag actaaagctc
     7321 ccattattat aggttgcata gttgtagctt gcattattat atttccaaat gaagtcataa
     7381 aagcagaaac agctggacca ataagttcag ctacaagtac tccaattagt attgtcacag
     7441 caggtgtaac caatatatct atttttgttt cttttgaaac taattttcca acctctacag
     7501 caaaaagtgt agcgacaaat gctccaacag gtcctcctaa ttcattacca caagctccaa
     7561 caagtgcaca agaaaatagt acaagtggag gtgcttgaag tccatacgca atagccactg
     7621 ctatagctgg accagtaacc tgtttagcaa gagggcttat aacttcagta aaaagagaaa
     7681 tattgaaatt atctcctaat gtattgaaaa ttgttccaat taaaagggac gaaaaaagac
     7741 caagtgccat ataacttagt gcgtcaataa gatacctttg aacagaaatt tcaatatttt
     7801 ttcttttagc aaatgatttg aaatttcttt ttttataatt gatttccaat ttaaaatcct
     7861 cctcatataa tataagtata atttcttttt aattaattat ataacttttc agctgacaag
     7921 acaaatgtta tttcactaaa attagactat tgtaatagta agaaattaac attaatttta
     7981 gttttaaatt ttaaatttat ggtttataat tagtataggg atattctgaa atataataat
     8041 tttccagttt atcccaatat ttaacttagg tagattgctt acaaaacaaa tgattatata
     8101 ttaggctaaa aggaagtgga tagatgatat ttgtaaaaaa taagaaatac agaaaaattg
     8161 ccataattat atctattcct atattgcttg ttgcgcagta ctaccttttt aaatttggaa
     8221 tacttagacc aatggtaaag ggagtagaaa ttgaaattgt agatggggaa tatgttaaag
     8281 atatagataa atatgtagta aaattaaatg aaacagttac tttgtctaca ggtgaatata
     8341 ttaaggttcc ttcgtatgct aaaaatccaa atatttggtt taatgtattg gataactcag
     8401 ggactttaaa aatagatgga aataaaatga cagcattaaa ggttgggtat tcttctgtgg
     8461 gtatcatgaa aaattcaaga gtattaaaaa aggctactat aaaagtagta gacccagagg
     8521 tagaatcact agatatggag attaatgggg atttaaaaca tgttggtgat agtgctacta
     8581 tagaaagtac aatagaagtt gattatgata agtttaaaaa atcatataag ccagtttata
     8641 agagtagcaa tgaaaatatt ttaaaaatca aaggaaataa agtaagtgca gttggagttg
     8701 gtaaagctac aatatatgct aagtgtggaa ataaagaaat tgagagaaca tttaggattc
     8761 aagctagagt tgctgaaata aatatggatg atataaaaat agaagttgac caaaatgaaa
     8821 aattgaaacc agatataata acttctccac ctggtttaga accaccaaaa atatattatg
     8881 agttagtaga ttcaaaatta cctgtagaaa gagcaataag tttaagtagt aatggaaatg
     8941 ttgtaggtct tagagaaggt aaagataggg tgagaataac ttgtggagat aaatctaaag
     9001 taataacagt aacagtagtc aaagaaacta taacgaatga ctatatacaa aacttggttg
     9061 gttcacataa aatagtaaac aataaagtta ttattaaact tgaatgggat tatatgaaag
     9121 atgtctttga ttatgatata tatcttaaag ataagttgtc aggagaatca tttaagaaaa
     9181 ttaaatctat aactataaat gaaagtgatt taggtaagtc taacaaggta tatgcaacta
     9241 tagaagtaga tatgaagggt aatcaaaata tagattttga tatttatgtc atagggaaaa
     9301 aggattcaga tacaactaag ccaagtaatg taataaatat tactaatgga aatggaaatt
     9361 caaataatga taataatatt aaagatatga gggtagaaaa tatatcagct aatgttgata
     9421 gagataataa tgtcattaga gttaactgga gtagtatatc tcataaggac tgtacttaca
     9481 gtatatatgt aaaaaataat actaatggag atggagaatt taatctatat caaagtgata
     9541 tacagggaaa tgagcatact ataagtattc ctgaaggaga tttaaatcta gatatttatg
     9601 ttgttgcaaa ttataatgga aatagctctc aaaatagtga tgttataaat gtaagataga
     9661 tttgtataca aattgaaagt gttgagttta tttaaatatt aaattaagaa aaaggatagt
     9721 atagggtaat tatactatcc tttttttgtt acaatatata ggtttaaaat ctctagtaat
     9781 ataaattata aaaagtctta tttacacaaa tctatagata ttttttttat taatcaatat
     9841 tgtcttaaat tgaacttagt gcggcttcta ttgcatgtat tactgtcgta tcaaatagtg
     9901 gaatagaagt gtcattttgt ttaattaaaa gacctatttc tgtacatcct aatataacac
     9961 cctctgcacc ttgtttgcca agcttatcta taatagataa gaaagctttt tttgaagatt
    10021 cagatataat tcctaagcaa agttcgttaa atattgtgtc atttactatt atacggtctt
    10081 cttcatttgg aattaagact tcaattccat tattaataat tacattttta taaaaatctt
    10141 gttccattgt atatttagta cctaataagg ccactttttt tatttctttt tcccttaata
    10201 ctgtagcagt tacatctgct atgtgaagta atggaatatg tatactttct tgtattttat
    10261 ctgatacctt atgcatagta ttagtacaga taacaataaa atctgcccct gcctcttgta
    10321 atttaattgc agcatcagct aaaattttgg cgcttttttc ccaatttcca ctagattggc
    10381 attcttcaat ttcttgaaaa tctacactgt aaagtataca tttagaagag tgtagtccac
    10441 ctaatctttc ttttattact gtattaataa cttgataata tgtaatggta ctttcccaac
    10501 tcataccacc aattaagcct atagtcttca tattatacct ctcttattta tttattttga
    10561 aatagctatt tacaaataca agtatagcat ctaaaataga agcatacaat aataaaattg
    10621 tatggagtac aattataatt aagaggagta attatatgcc aataaattct tttgaaaact
    10681 atcctatgac atggaaacca aaattagata accgaaaacc accaatatat aaaactttag
    10741 caatgttatt agaagaagat attaaaagag gaaatttaaa tcctggagat aaactgcctc
    10801 cacaacggga attagcagat tttctagatt taaacttaag tacaataaca cgagcattta
    10861 aactttgcga agaaaaggga ctaatatgtg caaaagtggg aaagggaaca ttcatttctt
    10921 ctgatgtaaa tgtaagtaat acattattat atcaaacgga atcaaaagac ataattgaat
    10981 tagggacagt tcatcctcca tatgagcaaa atacatatat tattgacttt attaaaaatg
    11041 tactaaaaca accagaaatg gataggtttt tacaatacat gtctccatct ggcacatata
    11101 tgcaaaaaaa gtcaattgca aaatggatag aaagaaataa tgtatacacc agtgaagaaa
    11161 atatattatt atctacagga gggcaaaatg ctatatgtgc aactttacta ggattattta
    11221 aagctggtga tagaattgct acagattctt taagtttttc tggaattaag tcaatagcta
    11281 aaatgatagg tattcaatta gtcccaattc ctcaagaaaa taatgaaata tcgattgaat
    11341 atcttgaaag ttattgtaaa aatgaaaata taaaaggcat atatcttata ccagattatc
    11401 ataatccaac gacacatact atgtctgatt tatctcgtaa aaagatagcc aagatagcaa
    11461 agcaatataa tataataatt attgaagatg ctataaatag catttttaga gacggtatac
    11521 aaactcccat attttcttta gctagtgata atactatata tatttttagt acatctaaat
    11581 ttttatgtgc tggtcttaga gtagcatttg tagttgctcc aaaacgatat atagaaaatt
    11641 tagaaaatgc actatataat atgaatctaa tggtttctcc atttacagca gaaatagtac
    11701 atcgtttatt atattctcct attatagata aaattataga agagaaaaga gatgcaatta
    11761 tagaaagaaa tgaagttgct gataaagttt tgtcagatta taatttgatt ggtgataaga
    11821 attgcagttt tcgttggtta cttcttcctg atgaattgga tggaaaatca tttgaaattt
    11881 ctgcaaaaaa tctaggagtt caagtatatt gtgctgaacg attttcagta ggaaactcta
    11941 cagttccaaa agcagttcgt atttgtgtaa cagctccaaa agatgtagaa gaattagaaa
    12001 aagggcttaa tataataaag tcacttctta agagttaaat ttattttact attatttaat
    12061 taaataaata aaaaggcgga gaaatttatt ataataaacc tccgctttta ttatttaatg
    12121 aaatacttta ctttttatta aaaaatttaa ttttaataat cttgaaaata taatcgtttt
    12181 atggtaaaat aattcttaaa aatagaaaaa attaatcatt tgttaaaaaa aatgcataat
    12241 ttattgtaat ttattgacga aggttgaaaa atattcataa atgtgcgaaa taacagaaaa
    12301 aatgcaaaaa ttaattcaat ttcatttgtt ataaaatcta tatggcaaaa ttaaatctgc
    12361 ctatcagaat gatataaaca catcagaaag gaggacgcta tcaaataata caagtagtta
    12421 actttgatag caaaaaatta tgaatgaaca aactagaata tccttagaga gagctgctga
    12481 attaaaatca aaaattgatg attatattca ggctagaaaa acgattaaca gaggtcttga
    12541 aaaagaagaa gagataaata aacgaaaaca gaaaatatta agtatcttaa atggaactga
    12601 agaggattgg aataactaca aatggcaatt atcaaataga ataacagatg tagatacttt
    12661 atcaaaaatt ataactctaa ctaaaaaaga aaaagaatat ataaaagagg ttggtactca
    12721 atttagatgg gcaatatctc catattattt gagtcttata gacccagaag atatatgtga
    12781 cccaataaaa ttactgtcta taccaacaca tatagagttg gaagatgagc aagaagattt
    12841 ggacccaatg ggagaagagt atacaaaccc agcaggatgt ataactagaa gatacccaga
    12901 tagattaata ataaatgtaa caaatgagtg tgctatgtat tgtagacact gtcagagaag
    12961 aagaaatatt ggacaacaag attctcataa gtcaaaagct attatccaag aatctataga
    13021 ctatatcaga gaaaatgaag aaataagaga tgtactagta actggtggag atgctcttac
    13081 attaaaagat gattatttag agtggattct tagccaactt aaagagatac cacatgttga
    13141 ttatgttaga ttaggtacta ggactcttgt tacaatgcca caaagaatta cagatgaatt
    13201 ttgcaatatg ctaaaaaaat atcaccctgt atatataaat actcatttta atcatccaat
    13261 ggaaataact aaggaatcta aagaagcttg tgaaaagtta gcaaatgcag gagttccatt
    13321 aggaaatcag gcagtattat taaatggaat aaataatgat aaatttgtaa tgagatgttt
    13381 aaatcaagaa ttactaaaaa taagagtaaa accttattat atattccaaa gtaaacatgt
    13441 aaagggaaca aaacatttca atacatcagt agatgatggt cttgaaatca tggagtattt
    13501 aagaggatat acatcaggaa tggctatacc aacatatata gtaaatgcgc caaaaggagg
    13561 aggaaagact cctttgcttc cgcaatacct tgtatcaaaa ggaacagatt acgttatgct
    13621 tagaacatgg gaaggaaaag ttataaaaat ggaagatgaa cctgctgtag atataaagaa
    13681 acttataaaa gagcaagcac aggattaaaa aagtatttaa agtttataaa aagaaacaac
    13741 tttaaaataa aaagaaaatg gatgtcaaat ttatatcaat ttgcatccat tttttattag
    13801 ttatttatta ttttgttgtt gattaactaa ttcaagatat ccacttgggt ggaagtaata
    13861 tatctcagaa gtattttcat ttacaaagta accaatatca ccatctgcat ctggtaaaaa
    13921 tacataacca ttaagatttt tagcttttaa tgcttcaaaa tcactagcat ttccttgata
    13981 tatgtaatca acttcattgt tgtattttac taaaagttct ttagcttgtt cttgagtcaa
    14041 tttagtattt tggcttacag gaacattctt ttgagaatct gcataagaag attcatttac
    14101 tggtgcagtt aataacaaac aagataaaac taataaagat ataagtgctt ttttcatact
    14161 aaaaccctct ttcatgttta attatataag attcattact taataatatt gtatgtttcc
    14221 tataattaaa tattaccatt aaagagaata atattacagt tacacagctg taacttttga
    14281 ttacaaaatc gtaattaaat tgtattttta ttccatttgt attttatcat gtctacgatt
    14341 tacaaactca cagaagtata tctttataac gcccatcaca ggagcagata gaatcatacc
    14401 aggtatacca aagaatcctc caccaacagt aactgctagg attgtaaaga atgggctaag
    14461 acctagttgt ccacctacga tttttggctc tataaatgta acttcaactt gttgaattat
    14521 tattaagtaa attaaagaag ttaaagcaat tgttggattg gagaataaat taattattac
    14581 aactggtgcc atacctatta ctggaccaaa atatggaacc atattcatga cacccataag
    14641 tgtaccaaat aatagtgcat attcagattt taaaaagtaa agaccaattc cagaaagtac
    14701 tccaactata aaagaatcta aaatcttacc tgtaaagtat ttaccaatat ttaaatttaa
    14761 gctttgacat agttctatta taaagtctcc atatttttta cctagggaaa tataaactac
    14821 tttttttgta aacagtaaga atttttcttt ttctaataat atatagaaac aaattataaa
    14881 acctaaaaga aattgtacta taaattttcc aactgaaaat gtagtgctaa atatcccacc
    14941 aagtgaacca ataagcaagt tactaaattt aggcattaca ctcataattt tgtcaccata
    15001 ctcttttaat gtattagggt caactgattg taaactctta cctaagtcga ataagaactg
    15061 ctctgtttta tctatgtatg taggaatttg agcaactaaa tcagctaaac tatttactat
    15121 ggatggaact gtaaataata taaaggaaat tagtataact atgagtacac cataagtaag
    15181 aagtaacgaa tatattctct taaaatttaa tttactttct aagaacttta ctagtgggtt
    15241 gaatatatat gctaatacaa atgctattat aaatggtgtt agtagtgaca ttaataaact
    15301 tatcactcca aaaaaatatt tgtaattatc aataacttta atcaagatat atgaaacaac
    15361 tacagtgagt acaatattta gataaatttt tttatcttgc aaaataacac aacctttctt
    15421 atatttatta aatttagata tacttaaatc taagttaaag ttaatattta tagcttatta
    15481 tacaataaaa agcagtatta atatagtatg attaatatga aattataaat acgttaaaac
    15541 tttatgctta tagctaaaat aataaaaatt atttatatta tgtttatata taatctaaaa
    15601 tgaaaaaaag tggtgccttt gattaaacta agtttttata atttaaattt tgtacaataa
    15661 gctatctttt attattcttt aaaatatata tttcatttaa ttatgacaaa taaagataaa
    15721 aattaaatat tttagaggtg attttattta gatatagaat tttagaataa tactatacaa
    15781 atgtattgta ttattatttt tatatcaaaa atagtaataa agtctatata catttagaaa
    15841 atactgttta aaaggagtag gatttatgat aaaaaaagag atatctaact ttagaggaag
    15901 tagtgattat gtagtatcac cagaattgat ggcatcagtt aatgttgcta ttgctcttga
    15961 aaagccactt ttgattaagg gcgaacctgg aacaggtaaa actatgctag cacaagcaat
    16021 ttcaaatgaa ttaaaaaaag atttagtaat ttggaatata aaatcaacta caaaagcaca
    16081 agaagggctt tatgtatatg atactgttca aagactttat gatagccaat ttggaggaga
    16141 aggtgtagat gatattagca agtacataaa atatggtaaa ctaggagagg cctttagctc
    16201 aaatcaacaa gtaattcttt taatagatga aatagataag gcggatttag aatttccaaa
    16261 tgacttgctt tgggaattgg ataaaatgga gttttacata aatgagacaa aagaaactgt
    16321 aagagctaaa caaagaccaa tagttattat aacttcaaat gcggaaaaag aacttccaga
    16381 tgctttttta agaagatgta tatttcatta tatagaattt cctgatagag acatgatgga
    16441 agaaatagtt aaagtacatt ttgataaagt agaagaacac ctattggaac aagtaatgac
    16501 tactttttat tggattagaa gtttaaagga catacaaaaa aagcctagta catcagagtt
    16561 aatagactgg atacaagcat taactttgag tggaatgcct attgaaaaaa tagaaaaaga
    16621 agttcctttt gcaggaatac tacttaagaa taatgaggat attgaatcta tgcaaagaca
    16681 cttatagtta ataggagctg gataattatg tttattgaat ttttttattt attgaggtca
    16741 agaggattaa atatatcttt aaatgaatgg atgactttaa ttgaagcatt ggataaaggg
    16801 ctgtgctatt caaacttttc aaatttttac tatctttgca ggatgatttt aataaaaagt
    16861 gaaagtgatt ttgataagtt tgatgcagtg tttttggagt attttaaagg tatagaacac
    16921 caagaagaaa ttccagaaga gattatgaat tggcttaaaa aaccagatat agatttagaa
    16981 gagtttgaaa gattggaaca aaatccaaat ataaatcttg ataaattaag agctaaattg
    17041 gaggaacgaa taaaagaaca agactctcct cataatggtg gtaattattg gataggcaca
    17101 ggaggcactt ccgagttagg acattcagga aaagggcaaa ctggtatacg tataggtgga
    17161 aattctactt atggtagagc tgttcttgaa gttgcaggag aaagaaaata cagagatttt
    17221 agagatgatg aagttttaaa tatgcgtcaa tttcaagttg cactaaagag acttcgtcaa
    17281 ttttctacta gaatagatgc tccaaagaca gagcttgatt tggataagac tattgaagag
    17341 acttgtaata atgcaggata tttaaagtta ttttttgaga agccaagaaa aaatacagtg
    17401 aagcttttat tgcttatgga ttcaggtggt tcaatgaggg ggtatagcac tttatgtaat
    17461 acattatttc agtctgtaag taaatcaaat cattttaaag atgtaaagat atattatttc
    17521 cataactgta tttatgatag attatttaca acacctgaat gttggttaag taaatctatt
    17581 aatactgaat ggatattaaa aaatatagat aaaaactata aagttataat tgttggagat
    17641 gcttctatgt cgccttctga gttacttcat attggtggta actatcgtgg tccatataat
    17701 tacacacctg gtatagaatg gcttaaaagg tttaagagaa agtacagtaa aattgtatgg
    17761 atgaatccag aacttagaga tggatgggat tcaattaatt attggtacca aactcaaaga
    17821 atgatacaaa gtgaatttaa tatgtttcca ctgactgtaa aaggtcttga aaaagcattg
    17881 aaaaatttaa tgctttcaag ataaagtaaa tataaatgat ggtatgattt attcaaaatt
    17941 gtaccatcat tatttatttt ttaatatatc tattataaat tgtataactg aaacttaaaa
    18001 aattatatag tctaatacat tttagactta cacaaaacat atacattaat aataatttta
    18061 gaagtcgagg tgattataat aaattatgaa ttaattgtaa aatacaatgg agatatattg
    18121 agattagaag aagaattagg tgtatcagta gagatactta attcatcata tgcaataata
    18181 acatcaagta atgaagaaga tgtaaataca cttttaactt atccagaaat tgaatttata
    18241 gaaaagcctt ttatattaca gactcaggat gtgcaaagtt tttcaagtac aggaataacg
    18301 gggtttaaaa atagaactgg tttaactgga aagggaacta taattggtat aattgattcg
    18361 ggaatagatt atactctacc tgtgtttaga gatagtgatg ggaggtctaa gatattatat
    18421 tattgggacc aatctattca aggaaatcca ccagaaggtt ttagagaagg aacattatat
    18481 actaatgaag atataaataa tgcaatagat ggaagtatgt atatacctat ttcaacaact
    18541 agtttacatg ggacacatgt tgcaggtatt tgtgcaacta ttgcaagtga tgccagaata
    18601 atagtggtta gagttggaaa tatacagacc gatatttttt cgagaagtac agagtttatg
    18661 agggcaatta aatttatttt agatagggca ttagaattaa gaatgcctgt tacattaaat
    18721 ataagttatg gaagtaatga aggttcacat agaggaacat ctttatttga acaatatata
    18781 gatgatatgt gtttattttg gaaaaacaat atagttgtag cagctggaaa taatgcagat
    18841 aaaggaggtc ataaaaggat taggcttcaa aataatatta cagaagaagt tgagtttata
    18901 gttggggaag gagagcgtat attaaacata aatatatggc cagattttgt tgatgatttt
    18961 agtgtacatt tggtaaaccc atcaaacaat caaacacaag caatctctct aacttcaggt
    19021 gagataagaa atactttggg tgaaactaga ataactggtt atttttaccc tatagcaccg
    19081 tactctctta caagaagagt tactctacaa ttaagttcaa atacacagat aactcctggg
    19141 ttatggaaaa ttgtatttga accaatagat atagtaactg gtaatgtaaa tatatatttg
    19201 ccaacttctg aaggactaaa tagaaatact agatttctga ttcctactca agagcttact
    19261 gtaacagttc ctggaacagc aagtagagtt gtaacagtag gtagttttaa ttcaagaaca
    19321 gacatagtat ctatattttc tggtgaagga gatacacaat taggtgtttt taaacctgat
    19381 ttattagctc caggtgaaga tataatatct tttttaccag gaggtacaag tggagcatta
    19441 actggaacta gcatggctac acctcatgtt acaggggttt gttcattgtt tatggaatgg
    19501 ggaattgtaa atggaaatga tttatttttg tattcacaga aattaagggc attactttta
    19561 aagggagcaa ggagattaag taatcaatct tatccaaata attcatcagg atttggtttt
    19621 ttaaatttat cagatataga tttgtacact ctatctagta taaatcaaga tttagaaacg
    19681 gaggatattg gatatagaag tattaataag tcatttaaag atgaggaaaa taggtataag
    19741 tttatagatg gttataatat acaaatacat aatgacttag agaatgaaat ttatataagt
    19801 aaaaatgcaa gtagacaatc aggaatacta tcaggaatag atgtagtcca tactcccgaa
    19861 tttgaagaag aactagctgg gttaggtatg agtcaaaatt tttttaagat aagtgattca
    19921 ttaggtgtac tatctataaa taatacagac tatagtagta tacaacgagt tttgcaactt
    19981 ccttcaatca taagaactgt atcgactaca aagatgacat tacttggaga aattaatcga
    20041 ggtacttttg gaggtgtagt tgcaacagaa gaaatgggcg taaatttctt taaaaataat
    20101 cctaatatta atataacagg aagagggact ctaatatcaa ttgcagatac aggaatagac
    20161 tatctacatc cagattttat ttatccagat ggtacttcaa aaatagtata tttatgggac
    20221 caaacaaaag aaggaactcc acctgatggt ttttatattg ggacagaata tacacgagaa
    20281 gatataaatc gagctatcgc agaaaatgac cctagtctat ctcaggatga agttggtcaa
    20341 ggaactatgt tgagtggtat atgctcaggg cttggaaatg taaatagtga atatgcaggt
    20401 atagcagaag attctgagct aataataata aaactcggta agatagatgg tttttataat
    20461 agtgcaatgt tgtttgctgc atctcagtat gcatataaaa aagcttttga acttagaaga
    20521 ccactagtta taaatatgtc tttaggtacc agtagcttgg caggtttaac caataggagc
    20581 aatagtgaaa aagcattctt tactagaggg ttatgtataa cagcaggagc aggaaatgag
    20641 ggcaatacac agacacatac atctggaata ataccacatg ttggaggttc tgttgaagtt
    20701 gaattagagt taaatgaaga cgaggaagaa ttatcattag aactttggtt aaatagacca
    20761 gataaagctg atgtaataat agtatctccc actggtgaag aaagtaaaag tgttggaatt
    20821 tcaaattata ataaagtaac aggtttattt gatttagagg ggactgaata ttctataacg
    20881 tatatatacc caacaacttt ttctgggcag caatttacta atgttacttt aaagaatgca
    20941 aaaagaggtg tatggaagat tcgattagta ggggtttata ttataacagg aagatataac
    21001 ttataccttc caaatagaga acttttaaaa agtggaacta gatttagaga ggtagaccct
    21061 ttttacacta taaattatcc tgctattcaa gatgatttaa taactgtagg agcctacaat
    21121 actattaatg gtagtttatg gcaaagttca tcaagaggac caactattga agacaggtta
    21181 aaaccagata tagtagcacc aggtgtcaat ataatagctg cttatcctgg gaacacgtat
    21241 gcaactataa ctggtacagc agcagcaagt gcacatgcag ctggtgctgc tgcaatgtat
    21301 ttccaatata cttttgttga tggtagatat ccaaaccaag catatgtgca aaaaataaaa
    21361 acttttatgc aagctggtgc tagaaaagat tcaaacacag tatatccaaa tacaaattct
    21421 ggatatgggc ttttggatgt tagaggaatg tttgatgttt taagatagga gggatttatg
    21481 gaaaaatctt attgtataat ttatcaaggt gatatagaat ctgccctaca agaaaatgga
    21541 ataaataggt atatggtttt aaatagtcag cttgcagtaa tatatgtacc tgtagatttt
    21601 gatgaaacta ttttaaataa tataatacaa gttgcttggt gggaagaatc tgaaccaatg
    21661 agcagtctaa ttgaaataac taataatgta aataatggag aaaccattac aacagcagct
    21721 gaaactgatt atatttatga aaatccatat aatgatataa caggaagagg aattttatta
    21781 gcagttatag attctggtat agattatttg catcctgatt ttataaatga tgatggaact
    21841 agtaaagttc tatatttatg ggaccaagaa gcaaatacaa atccacctcc agaaggtttt
    21901 atttttggaa gtgaatttac aagaagtcag ttaaatatag ccataaatag aaatgatgga
    21961 agtttgagtc aagataatat aggtactgga actttagttt ctggtatatt agctggaaat
    22021 ggaaggataa attcgcagta tagaggtatt actacagaat cagacttaat agtagttaaa
    22081 ttaaaatcat atacagatac ttattatgct ggaaggataa attatagtgt ttcagatttt
    22141 ttagcagcta taacgtatgt tactaatata gcaagaacag aaaataaacc actaataata
    22201 aatttgacta ttggagttaa gtcaagtgct gtagcaacta cttcaatatt agatactttt
    22261 aatatattga gtagtgctgg tgtggtagta gtgagtggtg caggaaatca agggaataca
    22321 gatattcatt attctggcag atttagcagt gtgggagaag ttcaagatgt aataatacaa
    22381 gatggagatg actatgctct tgatataaca ctaaatacga atggtccaga taaagtggga
    22441 gcacagataa tatctccttc aggtgaagtt agtcatgata taaggtattc tccagacttt
    22501 tatatatata gaggaaaatt taacttagaa aatactactt atgcaatgag atttatttat
    22561 ccatatatta cctctggaaa agaaaatttg gagataagat taagagatat aaaacctgga
    22621 gtatggattt taagattgac ttcagaactt ataataagtg gagaatatga catatactta
    22681 ccaaataaaa atttgatagc tccagacaca aggtttttag accctgattc agtagctact
    22741 ataactatgt atgctgctag tgatgatgta attacagttg gtacatttaa taataagact
    22801 gatagtatgt ggattggttc ttcgaaagga ccaattagag gaagaggaat taagccagat
    22861 atagtagctt caggtgtaga tataatatcc acatataaaa atggaacata taatacagga
    22921 acaggaacag gagtgagcag ttctatagtc acaggtgttt tagccttatt gatggaatac
    22981 ttagaaaaac aggataatgt accgagatta tcattgttta cacaagtttt aaaaacatat
    23041 ttaatattgg gtgctacaaa acttgagata tatacatatc caaatgtatc gcagggatat
    23101 gggattttaa atttaaaaaa tacgattcaa cagatagcaa atactctata gataagaacc
    23161 tatgtaaaat tataattgat tagtaattta attgtatcaa atactttaga tagagtagaa
    23221 aaatggcata aaaaataaat atgaaaatta aaaaaacagt tttatatttt gaatacacaa
    23281 agtaaatatt ttgtatataa ataggtataa attaagtatt gaaaagatat gatgttaatt
    23341 taaaaatcat tataatttag acttatgaat tttatttgta aaattgctaa aactattaca
    23401 aaataaataa tagaggtgat agttttggaa agtaaaaagg caagagcatt aaaaggtaaa
    23461 gataataaaa gacttaaaag ttcactttta gttggaaatg agtcgacagc agcatgggct
    23521 gatgttgaaa aattaaaacc tcatagtaaa gtttcaatac cttcaattaa gggtgtcgaa
    23581 gaagcaaaag actgggttga caatggaagt aaattataaa attttggaaa tttaagtaat
    23641 aaagaagtaa tgaagataag tatttattat cattatgcaa ggtagaatta ttatatttta
    23701 aataacaatc agtatcaatt atataaattt attgactaaa aatttataaa ttggtataat
    23761 tttgttaatt agtagaaata ataaaagata aatactgtga agagaagagt agatataaaa
    23821 tgtagttcta gcgagttgag gatggtgaaa gctcaatacg aggtttatat tgaagaacat
    23881 cttggagttt ctaatcgaaa gcttatttag taagtagact tagacggaag gcaccgttat
    23941 aggctttaag cattgcttaa ctaagaggtc attatgacaa ctagggtggt accgcgagag
    24001 taatagtctt tcgtcccttt atatagggat gaagggcttt ttttattgaa attatattta
    24061 aaagaaatta ataacaatta tactaggagg atttaaaatg caaaaggaat ttataacagt
    24121 aaaagaattg tacagagaaa aagaacagta catagggaag actgtaaaag tagctggatg
    24181 gataagaaca tctagagtat ctaaaaattt tggatttata gagctaaatg atggaagctt
    24241 ctttaaaaat atgcagatag tatttgatga gaaagtagaa aattttaaag aggtaggaaa
    24301 attagcaata agctcatcta tacttgtaga gggagagtta gtatctacag aaggggctaa
    24361 acaaccagtc gaaatacatg caaaaagcat agttgtagaa ggggaatcag atagttcgta
    24421 tccactacaa aagaagagac atacgcttga atatttaaga acgatagcac atttaagacc
    24481 aagaagtaat actttttcag ctgtatttag agtaagatca atagctgcct atgcaataca
    24541 caaattcttc caagaaagaa actttgtata tgcacattct ccaatcataa cgggtagtga
    24601 ctgtgaaggt gcaggagaaa tgtttagaat aacaactatg gatttaaatg atattccaaa
    24661 aactgaagat ggtaaaatag actatactca agatttcttt ggaaaagagg ctaatttaac
    24721 agttagtggt caactaaatg ccgagataat ggcactttct tttagaaacg tttatacttt
    24781 tggacctaca tttagagctg aaaactctta tacaggaaga catgcttcag aattttggat
    24841 gatagagcca gaaatagttt ttgcagacct tgaagataac atggaacttg cagaagatat
    24901 gataaaatat gttataagct atgtattaga aaatgctcct gaagaaatgg aattttttaa
    24961 tagttttata gataaaggat tgttagaaag attaaataag atagtaaact ctgagtttat
    25021 tagaataacg tatactaaag cagtggaatt attaattgaa tctggtcatg aatttgagta
    25081 tcctgttgag tggggatgtg accttcaaac tgagcatgaa agatatataa cggagcaaat
    25141 atataattgt ccagtgtttg ttacagatta tccaaaagat ataaaagctt tctatatgag
    25201 aatgaatgaa gacggtaaaa ctgttagagc aatggattta ttagttcctg gagttggaga
    25261 aatcgtagga ggttctcaaa gagaagaaag agaagatgta ttgttagata gaattaatga
    25321 aatgggatta agagaagaag attattggtg gtatttagaa cttagaaagt ttggtactgc
    25381 tactcactct ggatttggtc ttggatttga aagaataatt atgtatatga caggtatgtc
    25441 taacataaga gacgttatac ctttcccaag aactcctaaa aatgctgaat tttaataatt
    25501 atatataata aaacttatat aactttaagt ataaacacta aaatgacttg catatttatc
    25561 tattttagag atatgcaagt tatttttatt gtactataaa tatataattt gattactttt
    25621 attttgcgat ataatactta taggtttata taatagtatg taacttttta ataaaatgca
    25681 acacaattac gtccctgttc ttttgcagta tatagagcct tatcacaatt acttataaac
    25741 tcatttaatg ttattgcctt ttcaattttc tttgtataaa ctccaatact aacagttaca
    25801 cattttaaag gaacatcatc acgctcaaaa ttgcaggact ctataatttt tcgtaaacat
    25861 tctgatttaa gtttagcatc tgtctcatca cagttaaaaa tacatataaa aaattcctca
    25921 ccaccatatc gtatagcatg ttcattttca gaacttgtaa aatctaaaat tactttggca
    25981 atattttgta gacaatagtc tcccttttga tgtccataat tatcattaaa ttgtttgaaa
    26041 taatctatat ctatcatcat tccagttata ggttcttgat ttgcatggct gattaatttt
    26101 ttttgtagaa tatcttcaag gtatctacgg ttgttcattt tagtaaggtc gtctgtcata
    26161 actttgtcgt taagtaaatt atttatatga gatatttctt catattgttg actaattaaa
    26221 agtttatcat agtaagtaaa tacacgattt ctatatagca ttgtagaaac aaaaatggca
    26281 agggatgtta taaaaaatgt gttgacaata ttactaaact cattgcctgg tatagtttga
    26341 atcccaggta agaataaatt aagaaacaaa aagcatcctc caaaaactat tactgattct
    26401 ccaaatttta atactgagaa agctgcagct gctaacataa tataactaaa tacacttagg
    26461 tcatttccac ctaactggtc aagtagagta attacacaac cccataggca gaaaaaagtc
    26521 acataaatta tccataaatt tatataaaac gtacaacaat cctttttttt attccaaaga
    26581 taagaaaata caaacataaa gacagaagat acacaaaata gtgttacata catataaaaa
    26641 taccattggc gtcttggtgt ggcaaaaggg cctccttctc tagctgagat tgatatcatc
    26701 ataattattt gagatattac tattattatg caaatgattt tacccgtatg ataaagataa
    26761 ttgcagagtt ctttttcaac attttctttg tgttttttgt ctagaggaaa tccaaaaaat
    26821 ttttttattt tttctaagtt catataagcc tccattaaaa ataaagcata aaaataagta
    26881 ttcctttcta tcattagata tttggaatac ttcgtttatg atttaattat atcaaaatat
    26941 attgtgtgta acaatctaaa attgtgatag ataaaattta ttagctaatg ttgagtaact
    27001 ttattaatta atactttact ataattaaga tttacagata gtttgctgct gaaaataaag
    27061 aatagaataa atttaaatta agcataataa aatataaata ataaagttcg cattaacaaa
    27121 ctatttaaat atcaagacct gttaaaaaac aacagcaata aacaaataaa atagataaat
    27181 atatccaatt aaggtataat taagttttat atgatatagt tatatttgat aatttacaat
    27241 agtaaaactt ggagggaaaa aatgggtgaa aagaaaataa ataatatgaa ttattcacat
    27301 aataattatt ccaaaaagac aggtgaacca actagaaaaa taaaaaatag ccatatcact
    27361 cttaaaagta aagaagaaaa aatttgttct agttgcaata aagaaaataa tactaaggat
    27421 aattactgta aattttgtgg aaatgaactt tatgaaatag catcattaag accacttgaa
    27481 acaaaactag acttgaaatc taaaataaaa gagctttcat atcatgcaaa taaaagaggt
    27541 gtatttttaa caacatttac aaccatattt atattgttta ttattgcatt gatatttaaa
    27601 gctattataa ctattcaatt taatgatatt agttatttaa taaatccagc acatataatc
    27661 ctagctctca acttgggtca aattagtgta tctatgtcta ccatgatggg ttctgggttt
    27721 ataaatgcca atataggttt gttgatatta cttataatcc ctattttggt attattaata
    27781 tcaaacctaa tttttatgag aaaaagatgt agagattcaa aaactgtatt agcaaattct
    27841 ctaggagttg gaatttttta tggattagtt ttagctattt tatctatttt tactaatgta
    27901 aaaactagtt cacatagtat gttagaatat ggatatgcat tagaatatag ttatgaattt
    27961 tttagtgttc ttttaaatgg atttgtatta gggtttatat gtacatacat aactacttat
    28021 aaaaaaagtt atgaaaagga aaatatgtat ttatctcttt tttcaaattc tataagaaca
    28081 ttcatattag gttatgtttt agtattagta atactattgg tactaactat atctgatagt
    28141 agttacttaa atgaactaga tatgagtagt tattcaaatg gattaaattt atttactata
    28201 ctcccacaaa tagctagtta tatgtgggct tttgcaaatg gaatttcagt aaccataata
    28261 aactcaacag tatcaatgtt tactttaagt tcttcatctt tatttggaga tactaaatta
    28321 atgttttatg caatgggagc attgtctatg ttaatattat tattaaatgg atacaagcta
    28381 agatttaagt ataatacaga tagtataaga cctataatag tatttagtat atattatgca
    28441 tttttgatgg gaatattggc tttatttagt acatttatac tagatagtaa tattaatttc
    28501 tttaatacaa ctaattatgg aacaacttta ataatgcaat ttaaagtctt acaagctatt
    28561 gtaatttcat ttgtatatag ttttgtgata tctttaatag gatataagct aaattcagct
    28621 gattaaatat gattggaggg aatttctgtg gaaggaaata ctaataataa agtagatgat
    28681 aaattttcta aggttaagaa aaagtttggt agtggaaaat ttaaagttga attatataaa
    28741 gaaaaatcaa aattaaatag agagattgat gatattcaag aaaagaaagc aaagatattc
    28801 ttggaaatgg gaatactgac atatcaaaaa attagagaag attctattaa tgataatagt
    28861 tttgataaat tctgtgatga gttactagaa cttgataagt taatttatga aaaaaatatg
    28921 gaaattaatg aaatggaaat ggcagaaagt aatgtaactt gtgagtgtgg ttatgttgga
    28981 aatgtaaatg ataaatattg tgctgaatgt ggtaaaaaat ttgaattgga aaatgaatgt
    29041 gatgatttta taatttgtgg ttattgtgaa agtgagatag atacagaggc tgaattttgt
    29101 ccatgctgtg gaagaaaaat tataatggat ttagaataat attgggaaac ctatgtatag
    29161 tatacatggg tttttttatt gaaattattt aaatggactt ataacagtag ttatataaaa
    29221 tgaaaataaa caccaaaaaa atgaaataaa atttctttta aatgtaaaaa acaagctaat
    29281 atataaaata aatcagtaaa aatatagatg cgagaaaaaa atatgaaaaa atattgcaaa
    29341 gaaaaagttt tcatgttatt attaatacat aaatcaagaa aataaatttc agaaacgagg
    29401 taatttaaaa aatgctagat aaggtaaagg gaagattaat agtatcatgc caagctttag
    29461 aaaatgaacc tcttcatagt ccttttataa tggggagaat ggctaaggct gctatggaag
    29521 gtggagctgt tggaattaga gctcaaggag ttgaagatat tattgaaata aaaaaagtta
    29581 ctggacttcc tgttataggt attataaaga gaaactatga agattcagat atatatataa
    29641 cgccaactaa aaaagaagtt gatgaacttt taacaacagg gtgtgaaatg atagcattag
    29701 atgctaccaa tagagttaga ccaaataatg aagacttaaa agaacttata aagtatataa
    29761 aagaaaacgg tgttctagta atggcagaca tttcaaatta tgatgaggct atcaaagcac
    29821 aagagtacgg tgtagattgc gtttcaacta cactatctgg atatactcca tatactaaaa
    29881 cattagaagg tccagatttt gtattaatgg aaagattagt taaagattta gaaatacctg
    29941 taatagctga aggaaaggta aatactcctc aagatttaaa aaaggtcttt gagttgggtg
    30001 ttcattcatc agtagtaggt tcagcaataa caagaccaca attaataaca gaaaaatttg
    30061 ttaaagcaat tgaaattaat taaaaattaa tttataaatt aaaaaattat tatatttaac
    30121 tatacttgaa aattaaaatt ggaggtaaag atatgagaac aacagatatg aagggaattt
    30181 attcagcatt attagtttca tttgataagg aagggaatat aaatgaaaaa gggttaagac
    30241 aaataattag acacaatata gatgtttgta aagtagatgg tctttatgta ggaggaagta
    30301 caggggaaaa cttcatgctt tcaactgatg aaaagaaaag aatatttgaa atagctaaag
    30361 atgaagtgaa agaagaaata aaattaatag ctcaagttgg ttcagttaat ttaaaagaag
    30421 ctgtagaact tgctaaattt acaactgatt taggatatga tgcaataagt gctgttacac
    30481 ctttttacta taaatttgat tttgaagaaa taaaacacta ttataataca ataataaatt
    30541 ctgtagataa tagattaata atatactcta taccattttt aactggtgtt gatatgagtt
    30601 tagaccagtt tggagaatta tttgaaaatg aaaaaataat aggtgttaag tttacagcag
    30661 cagatttcta cttattagag agaatgagaa aaacattccc aaataaatta atatttgcag
    30721 gatttgatga aatgatgtta ccagctacag ttttaggtgt agatggggca attggttcta
    30781 cattcaatgt aaacggtgtc agagctagac aaatattcga attaactaag aatgaaaaaa
    30841 tatcagaagc acttgaagta caacatgtga ctaatgattt aataacagat atattaggaa
    30901 atggcttata tcaaacaatt aaattactac ttgaagaaca aggtgttgag gctggatact
    30961 gtagacaacc tatgaaagaa gcaactgatg aaatgaaatc aagagcaaaa gaaatataca
    31021 gaaagtattt ttaataaaat acttttagta tgggaggatt agtcaaatgg taggatttac
    31081 ttggatagat tttgcaatac tagtggttta cttattggca gttttatttg caggattgtt
    31141 attttcaaaa aaggaaatga agggaaaaga gttcttcaaa ggtgatggaa ctattccgtg
    31201 gtgggttaca tcagtatcaa tatttgccac attattaagt ccaatatcat ttttatcatt
    31261 agcaggaaat tcttatggtg gtacttggat tttgtggttt gcacaattag gtatgtttat
    31321 agcaatacct ttgacaataa aatttttctt acctctgtat agtaggttag aaatagatac
    31381 agcatatgaa tacttagaaa tgagatacga aagtaaaggt cttagagtac ttggagcttt
    31441 gatgtttata atatatcaaa taggacgtat gtctatcata atgtatcttc catcagtggt
    31501 tctagctggt cttacaggaa tacctgtaaa tgtattgata gtagttatgg gtgtaatagc
    31561 aataatttat tcatatacag gtggattaaa ggctgtcttg tggacagatt ttatacaagg
    31621 tatggtatta atagtaggtg ttacagggac attgttctat ttaatagcaa gtataaatgg
    31681 tggttttgga acagtaatgg atactcttac aactggacac aagttcttag caagtaatga
    31741 agttatgttt gataaaaata tactatctac aagtgcattt ataatattta ttggagctgg
    31801 tcttaatact ttttcatcat atgtatcgag tcaagatata gttcaaagat ttactacaac
    31861 aactgatata aagcaactta ataaaatgac atatggaaat ggtgttttat caatgggact
    31921 tgcaacagta ttttatctaa ttggaacttg tttatttata tactatactc aaaacccaga
    31981 tttagctcaa acagttcaac aagaccaagt atttgcatca tatatagctt atgaattacc
    32041 agtgggtata acaggaatac tacttgcagc gatatatgca gcttctcaat caactttatc
    32101 gacagggctt aattcagttg ctacaagttg gactttagat atacaaacta tcataacaaa
    32161 agatataagt atggaaaagc aaactaagat agcacaatat atatcattag gtgtaggtat
    32221 actttctata gctgttgcaa tagtattagc aaatggagaa ataaagtcag catatgaatg
    32281 gtttaatagt tttatggggc tagtactagg tgttttagca ggaatatttg tacttggtgc
    32341 attttgtaaa aaagcgacta aaatgggagc atatgttgga ttcatagtat cagcaatttt
    32401 agttgtttat ttaaaatacc gtatgccaga agtaacttct tgggcatatt ctctaataac
    32461 aatcacgact tctgtagtgg taggtacaat agttagtgca atagaagcta aagttaaagc
    32521 aaaagtatct ttaccaaaag ctgagactac tgtttattat gaaaaataat atttaaatga
    32581 taaattgtct tatagagtga ttagataaat aatttagtta ctctataaga ttgtataata
    32641 tactttgtat ataaaagtat aaaaatagac tttataatta tatagggaga tagtgcttat
    32701 gatatttgga aatataaatc atagtaagac atattcaaca ttgcatgaag atttacttag
    32761 atgttttgaa tatgctaaag ataattcatt aatagattat gaaaaaggta cttatacaat
    32821 agatggtgat gatatttttg tgaatattgt agagtataaa acttgtgaaa aagaagatag
    32881 attctgggaa gctcataaga agtatataga tgttcatttg atgcttgatg gatgtgaacg
    32941 aatagatata aattttatag aaaatttaga acaaaaaata tatgaagaaa agggcgattt
    33001 cttgcctctt gatggtaaaa ataatggata catggaactt agaaaaggtg attttttagt
    33061 ttgttatcca gaagatgctc atatgacagg aataaaagtt ttagaaaaag aaaatatcaa
    33121 aaaagctatt tttaaggttt tggttagata atgataattt atataaccac tagggctgaa
    33181 aatagtgatt taactttcag cgattagtga gacttcgtaa attttagtta gattactatt
    33241 ttcagcctta gttttttatg agaaaattga aaataattaa aagcttaaaa ttttgtaaag
    33301 gagttaactt atggaaaata tagatgtatt cttattaata ggacaaagca atgctagagg
    33361 attgggaaat ccaaaagaga gcatcattcc aaatgaaaat tgttttgaat atttgagtac
    33421 tgatgaaatt ataaatatga gatgtgagct agagacttca gaaggtgatg gtactatagc
    33481 acctgcattt tcaaatgaat ggaataagct tactggaaat aaagtttgtt ttattcacaa
    33541 tgcaaaagat ggttcaagaa taaaaaactg gaatcatgat aataactggt ttctaaatga
    33601 cactatagaa aaatttaata caggctgtac taccttaagt aaacattata atatagaaaa
    33661 taaatacgtt atttggattc aaggtgaaag tgatgcaaaa tatggtagtg atgcattgta
    33721 ctataaagaa agtctaaaaa aaattgcgta taggcttaaa gaagaatgta tgattgataa
    33781 aatgtttgta agtttaactg gttattggct tggagaagat gaatatttta ttagaacaag
    33841 acgaattgca gcagctcaag aatctgcttg taatgaatgt gatatactgt gtgtaggaag
    33901 taaaattgct atgcaatttc atgacagagg attaactatt gatgatgttc attacactca
    33961 agaaggtctt aatatgttag gagaggactt atgtaaaaat atttataagt atcatataac
    34021 caaagaaaaa agtgtattaa gtgatactat agacttatca gaagcaagga aatatatctg
    34081 tgaattggaa aagataagta aaagatttgt gtaatataaa attttaaata gagttgttaa
    34141 tttataaaaa taatttgtat ggagatatgt tatgaaaaat tacgtttgta tagatgttgg
    34201 aggtacttca ataaaatatg gttttattag agaagatgga tttataattg ataaatcaag
    34261 tcttgatact gaagcaaagg aaaaaggtgg agaaggaata ttagccaaaa taaaagatat
    34321 agtaaaaaaa tatatagagg aaaatgaaat aagtggtata tgtatatcaa ctgcaggtat
    34381 ggttgaccca gttgaaggta aaatactatt tgcattagaa gaattaattc caaattataa
    34441 gggtatgcaa ttaaaaaaag aagtagaaaa agagtttaat ataaaatgtg aagttgaaaa
    34501 tgatgttaat tgtgctggac ttggtgaagt gtggcttggc gcaggtagag gtgcaacatc
    34561 ttctatatgt ttaacaatag gtactggaat tggtggatgt attataataa acaataaact
    34621 gataaatggg tttagtaata gcgctggtga agtaggatat atgaatataa atggaagttc
    34681 ttttcaagaa ttggcatcta ctagcagtct aattaaaaaa gtagcaaaaa taaaaaattt
    34741 gaatgaaaag gacttaaatg gaaaaataat ttttgattta gcaaagaata atgatgaaga
    34801 ttgtttaaaa gaactcgata atatgataaa atcattagca gttggaatcg caaatatatg
    34861 ttatgtaata aatccagaag tggtaatttt aggtggtggg attatggcac aagaaaagtt
    34921 cctaaaacca aaaatagaca aagcattaaa ggaggttctt atagagagag tttacaaaaa
    34981 cactaatata gaatttgcaa agagacaaaa tgatgcagga atgcttggag cattatataa
    35041 ttttttaaac aaaatataaa gaatagaagg gtttttaatg agtattttag aacaattgga
    35101 atcaccaact tttaaagtca caaaatcaga taaattatta atagcttata taaaagaaaa
    35161 tatagatgat gtattttata aaccaatatc acaaatagct aaagagagca atattggtga
    35221 agcaactata actaggtttg taaaaaaaat gaattttaat ggattacagg attttaaagt
    35281 tactttagca caagaaatat caactatcaa taaaaaaaat attataaata aaaatataca
    35341 aaatgatgag ccagctttgg atacagcaaa aaaactttta agttcaaatg taaccacttt
    35401 agagaacaca gtagagataa tcaatagtaa ggatgtacat gactgtgcaa ggcttataat
    35461 aaatgcaaag aaagtttact tcataggcat tggatattct ggtataatag cacaggattc
    35521 aaactataaa tttatgagaa taggccttaa ttgtgtaagt tttgatagca gtcatacgat
    35581 gataatgatg tcttccataa tggaagaagg agatttaata attgcaattt cacattctgg
    35641 tgaaacagaa gagattataa aaactgtaaa gcttgcaaga gccaataatg ctaaaattat
    35701 atcaattact gaaaataaaa attctgaatt aaaggatatt tcagatgtac acttatctta
    35761 tgtatcagga gaaacagtac tggaaactgg ttcaatatct tctaaattag cacaattttt
    35821 cattattgat ttagtatata cacaggtagt aaaagaactt tctaatgaag ctattgaaag
    35881 gaaaattaaa actacaaatg ctataaaatt gtttaaaaat gaataaaata agaaatttgt
    35941 aatgttttat agagctctct taaaaattta ggaatggaga gatattacat gtctaa
//
LOCUS       DAEPOZ010000011        35192 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.011, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000011 DAEPOZ010000000
VERSION     DAEPOZ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35192
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.011"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            438..1280
                     /locus_tag="KR440_000472"
     CDS             438..1280
                     /locus_tag="KR440_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBH0697540.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
     gene            1295..1759
                     /locus_tag="KR440_000473"
     CDS             1295..1759
                     /locus_tag="KR440_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697541.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIITCFGQMIALKTQAQDLLIGSISG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            1746..2918
                     /locus_tag="KR440_000474"
     CDS             1746..2918
                     /locus_tag="KR440_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH0697542.1"
                     /translation="MECFKEVVNMNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTNVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGKVALIKAIKVLAMCGVDILYSK"
     gene            2962..3897
                     /locus_tag="KR440_000475"
     CDS             2962..3897
                     /locus_tag="KR440_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HBH0697543.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(4696..5094)
                     /locus_tag="KR440_000476"
     CDS             complement(4696..5094)
                     /locus_tag="KR440_000476"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBH0697544.1"
                     /translation="MKWKELYNNARGKLNPRTISPFIDAGGVAAAILTDSGNVYTGVC
                     IDTACTLGMCAERNAIANMITNGESKITKLVCVMSNGSVGSPCGACREYLMQLDKDSA
                     CIEILVNVETEKTVKLSELLPDWWGSDRFI"
     gene            complement(5393..8086)
                     /locus_tag="KR440_000477"
     CDS             complement(5393..8086)
                     /locus_tag="KR440_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBH0697545.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQST
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANQG
                     VYVEPSIFTTITTFDGQLLVKNAPEEHKVVDPEVAYVVTDMLESVITEGTGGVATLPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     STTPPDNGGNNGGGSTTPPDNGGNNGGGSTTPPDGGGTPAT"
     gene            8300..8998
                     /gene="yunB"
                     /locus_tag="KR440_000478"
     CDS             8300..8998
                     /gene="yunB"
                     /locus_tag="KR440_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HBH0697546.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            complement(9210..10217)
                     /locus_tag="KR440_000479"
     CDS             complement(9210..10217)
                     /locus_tag="KR440_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBH0697547.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNSEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISNLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            10445..11329
                     /locus_tag="KR440_000480"
     CDS             10445..11329
                     /locus_tag="KR440_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH0697548.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDKKNKIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            11531..13081
                     /locus_tag="KR440_000481"
     CDS             11531..13081
                     /locus_tag="KR440_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBH0697549.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKEKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            13110..13745
                     /locus_tag="KR440_000482"
     CDS             13110..13745
                     /locus_tag="KR440_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBH0697550.1"
                     /translation="MKKFLNVLFSVCISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSEDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVFLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWIFNPNLDPVINILPEEFFFHSGMMILILILLASILLFVMYRLYKSFKIK"
     gene            complement(13856..14116)
                     /locus_tag="KR440_000483"
     CDS             complement(13856..14116)
                     /locus_tag="KR440_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HBH0697551.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFKYTDKGIKDCSSCMLPHKKDNYNYIMGKFQDLVKITSKK"
     gene            14333..15616
                     /gene="hflX"
                     /locus_tag="KR440_000484"
     CDS             14333..15616
                     /gene="hflX"
                     /locus_tag="KR440_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBH0697552.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTSESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYADLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGENGITLDVNFDEEDFNIYREYILEK"
     gene            15624..16301
                     /locus_tag="KR440_000485"
     CDS             15624..16301
                     /locus_tag="KR440_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HBH0697553.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEAGEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            16387..16830
                     /locus_tag="KR440_000486"
     CDS             16387..16830
                     /locus_tag="KR440_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBH0697554.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTNCVLAPEFQE
                     GGFYKVKDALDMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            17136..17780
                     /locus_tag="KR440_000487"
     CDS             17136..17780
                     /locus_tag="KR440_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBH0697555.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVVVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITNGTGKIILGEEFYLSEQNGEILI"
     gene            complement(17866..18243)
                     /locus_tag="KR440_000488"
     CDS             complement(17866..18243)
                     /locus_tag="KR440_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBH0697556.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(18243..18629)
                     /locus_tag="KR440_000489"
     CDS             complement(18243..18629)
                     /locus_tag="KR440_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBH0697557.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            18808..19563
                     /gene="nadE"
                     /locus_tag="KR440_000490"
     CDS             18808..19563
                     /gene="nadE"
                     /locus_tag="KR440_000490"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HBH0697558.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYRAEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            19646..20365
                     /locus_tag="KR440_000491"
     CDS             19646..20365
                     /locus_tag="KR440_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBH0697559.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(20533..21537)
                     /locus_tag="KR440_000492"
     CDS             complement(20533..21537)
                     /locus_tag="KR440_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBH0697560.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            21803..22699
                     /locus_tag="KR440_000493"
     CDS             21803..22699
                     /locus_tag="KR440_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HBH0697561.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREINLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            22837..24099
                     /locus_tag="KR440_000494"
     CDS             22837..24099
                     /locus_tag="KR440_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697562.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            24175..25368
                     /locus_tag="KR440_000495"
     CDS             24175..25368
                     /locus_tag="KR440_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBH0697563.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LKRCEHYKALKPEIEKWSVKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            25997..26791
                     /locus_tag="KR440_000496"
     CDS             25997..26791
                     /locus_tag="KR440_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBH0697564.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDIAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            26885..28225
                     /locus_tag="KR440_000497"
     CDS             26885..28225
                     /locus_tag="KR440_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBH0697565.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            28362..29594
                     /locus_tag="KR440_000498"
     CDS             28362..29594
                     /locus_tag="KR440_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBH0697566.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKNGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     ALIESGIVTMNKEACEIC"
     gene            29634..30776
                     /locus_tag="KR440_000499"
     CDS             29634..30776
                     /locus_tag="KR440_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBH0697567.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGTDIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGIGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASSTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            30787..31581
                     /locus_tag="KR440_000500"
     CDS             30787..31581
                     /locus_tag="KR440_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBH0697568.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFAPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            31592..32614
                     /locus_tag="KR440_000501"
     CDS             31592..32614
                     /locus_tag="KR440_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBH0697569.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELEVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEVLPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            32713..34089
                     /locus_tag="KR440_000502"
     CDS             32713..34089
                     /locus_tag="KR440_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBH0697570.1"
                     /translation="MKEQWYKDIFARILSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKDKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(34421..34765)
                     /locus_tag="KR440_000503"
     CDS             complement(34421..34765)
                     /locus_tag="KR440_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697571.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
BASE COUNT        12991 a         4434 c         6408 g        11359 t
ORIGIN      
        1 cacattccaa gaaaattata tccaaggtta ataatacgaa atagcgttaa aagaataaag
       61 tagtaagctt ttaagtatca aaagtttatt aaaaatatta tagattatgt gaagtttttt
      121 aacaaacaat ataaataaaa attaaaagga gtaggtgtat ataagtacat tgtactcctt
      181 tatttattga gatagttttt ataatatgaa tgaataattt tattcaatta tttgaaaatc
      241 cgttatttta acaatataaa ttaataaatt atttgtattt tatatcatga atctataata
      301 tttttttgat tcataactaa aagtatataa aaatgatgaa taaatatata ttatacatta
      361 tcaatgatag gttatataat aaaatcacta acaattatat tataagaaaa ttataaaaat
      421 tacaaggagg tatagaaatg aaagaaaaag cagggattag aaatgccata atagcagcct
      481 ttataatcac aatattggca gaattaattg gacctatttc atttaaagtt atgggaataa
      541 atgtaacttt acttagtata ctatgggcta tattcatagg aatggctgtt tctcctcatt
      601 tattagggag agttatacca gccttaaaaa agtttatagg ggataatgaa ataaatgtat
      661 ctccatattt attatctttg acactatatc cattaggaat aatgtttgga attaatgcag
      721 ggcctaaagt tggtatatta cttcaagcag gaccagcact tttattacaa gaatttggtc
      781 atatgggtac aatgttgata gcattacctg ttggagtagc attaggtctt ggaagaagtg
      841 cattaggtgg tactttttca ttatgtcgtg acacagcact aggcattatt ggagataagt
      901 atggattaaa ctcaccagaa ggtataggaa cactaggaac ttacataagt ggtagtatat
      961 ttggaacatt attattttca tttttagcac cagtgggtgt acttttagga tttcatccat
     1021 atgctttagc aatggcatca ggaatgggta gtggttcaat gatgggagct gcaacagcat
     1081 cattaatgaa tacggttcca caaatgtcag agcagatttt agcatattca gctactagtg
     1141 gattgattac agctgttact ggaatatata tagaattatt cttatcttta ccagtagcca
     1201 actactatta tagcaaagta gctccagtta ttgaaagatt tagaggaaga aaaacaagaa
     1261 gaacaagtaa taaattgtaa aggaggatat atatatggaa aaaactttta agactattat
     1321 ggagcagata aaaatagtat tgtttatata tattattact tgctttggac aaatgatagc
     1381 acttaagact caggctcaag atttgttgat tggaagtata tctggatttt tgttggtgat
     1441 aattgctatt ataataaaga attttgttaa aaaacctaat ttaccaggtt ttgcatgggc
     1501 tacattagtt gcattcgttt tatcgcttcc aatatctcca gtgggggata tcatagcaac
     1561 aaatgtaggg aaaatcaatt ttatgtctac agtaacacca ttattagctt ttgcaggaat
     1621 ctctgtaggt aaccaattag atatattaaa gactttgagt tggaagttag tagttatatc
     1681 atttatagtt atgacatcta cttactttgg ttctgctttt atatctcaaa cagttttaag
     1741 attaaatgga atgctttaag gaggtagtaa atatgaatga attaaaagca aaaattaaag
     1801 aggaaataaa gttattatct gaagagaaaa agacatcatt tgaaaaagtt tctgactatt
     1861 tattttcaaa tccagaatta gcatttgagg agtataagtc acaaaaagca ttatgtgatt
     1921 tgttagaaga aaatgaattt aatgtaacta aaggtgtagg aggattagaa acatcttttg
     1981 aagctgtgta ttcaaatgga actaatggga aaacagttgc ttttctagct gaatatgatg
     2041 cacttccagg aatgggacat gcatgtgggc acaatatcat agggacaagt tctgttggtg
     2101 caggtatcat attaaaagaa atcatgaaaa aacataatat agaagggact gtaaaggttt
     2161 ttggaacacc tgcagaagaa agagtaggtg gaaaaataac tatgattaaa gaaggtgtat
     2221 ttaataatgt agatgctgca ttaatcttac atccaagtga tgcttcaatg cctgatgata
     2281 tatcatttgc tcaagttaat ttaaaatttg attttactgg taaggcatca catgctgctg
     2341 cttttccttg ggaagggaaa agtgccttaa gtggagtaat agcactattt aacagtgtaa
     2401 atagcatgag attacatctt aaagattatg caagagtaca tggaattata actgatggag
     2461 gtagcataca taatataatt cctgaaaaat ctacagctat atttaatgta agagccttaa
     2521 gcatagaata tttaaatgag atttgtgaaa tgcttaaaaa ttgtgctaaa ggagctgcaa
     2581 tttctactgg aactaatgta gaaatagttc aattagatga aatatacaaa gagattaaga
     2641 atgattctga attagtgaat atagttagac aaaattttga agttttgggt gaagactatg
     2701 ttgaaagaga tttaagtcaa ggtataggtt caactgatac aggaaattta actcatgaga
     2761 tacctgcaat acaagcatat ataaaattaa aagaaaatac tgcaactcat acagatgaat
     2821 ttgctgttgc agctggtggt gaagaaggca aagtagcact tataaaagca attaaggtgc
     2881 tagcaatgtg tggagtagat attttgtata gtaaataata tataatttta aaatatatta
     2941 agaaaggaaa tcggattaga catgatatta aaacaagtaa tagaggttta tgacatttta
     3001 gataaagcaa atgctaatgg ggaagaagta aaaacttact tacaaggtta tggtgaagta
     3061 gatgtaactg taaaagaatt gtcttcaagt aaaggttcaa cagatttagt aaagcttaca
     3121 attcctggga aaaatggaaa attaaaaggt ggagatgctc ctactcttgg tattttaggt
     3181 agattaggag ggattggagc aagaccagaa gttataggtt ttgtctcaga tggtgatgga
     3241 gcattggtag caattgcagt ggctgctaaa cttttggata tgcaacgaaa aggggacata
     3301 ttagatggtg atgttgtaat ttcaactcat atttgcccag atgctccaac taaagaacac
     3361 tatcctactc cttttatgga ttctccaatt gatatgatga ctatgaatga aaatgaagtt
     3421 gatagtagtt gtgatgcaat actttctata gatactacta aaggaaatag agtaatcaat
     3481 actagaggat ttgcaatatc gccaactgtt aaagaaggct acatattaaa aactagtgat
     3541 aaccttttag atattatgca gactgtaaca gggaaatctc catttgtatt ccctttgtct
     3601 attcaagata taacaccata tggaaacaat ctgtatcact taaatagtat tttacaacca
     3661 gcagtagcta cagatgcacc aatagtagga gtagcaatta caacagagat gcctgttgct
     3721 ggatgtgcta caggtgcaac taattttagt gatttagatt cagctgggag atttgcaatt
     3781 gaagttgcca agctatttgg tagaaataag tgtgatttct atgataaaga agagtgggaa
     3841 atgcttatta agagatatgg caaattaaat agatttcaaa catttggtat acagtaatta
     3901 gaaaaatatt aagtttatat aatttgtgat acaactattt aagaagatat aaacaattag
     3961 gaataaaagt aagaataaag cacttagtta aaaactttaa aaaaaaatat ttatttgttt
     4021 atataaaaag gaggattctg gttattggga ttctcttttt tatgaataca aataatatat
     4081 tgtaattata aaaaaaggag cgtttcaaag tgattttttt aatcaccttg agacaactcc
     4141 ttatattttt tatttaaatt ctatttttta ttgactgtat agctattttc accatcataa
     4201 ggtggcatga ccttgaacag cgtggttttg taaagttggg caccacaaaa caaaaaatca
     4261 acaagccctt gtgtggtctg ctgacgtgtt tactatacta tactttccca aaaaccgcaa
     4321 gcactatggg aggctataag gcggcgacca ttcaggggcg atcccctcga aaccgggata
     4381 gggttcccat gtgggacata ataaagagcg ccccggaggt atcaactacc atccggggcg
     4441 ctctttatta tgtcccgtgg gaaccatgcc cacgaagcag ggcgtacaac ggacagatag
     4501 gtcctaccct cctgacagac tggaatctca atcccatcga aaacaatcat aacaatcatg
     4561 gcaagattat atacactcct gtaaaatatt tttgtgtgta ccatgaagta ctgcttgcca
     4621 ctattgagaa cggataaaag ggaagtacgt attataccaa taaggtgaac gaaacaaaat
     4681 actcatttga taaagttaaa taaacctgtc ggaaccccac caatccggca agagctcact
     4741 caatttaaca gttttttctg tttcgacatt aactaaaatc tcaatacaag cactatcctt
     4801 atcaagttgc attaaatatt cacgacaagc accacatggt gagccaacac tcccattgct
     4861 catcacacaa actaatttag taattttact ttcgccattt gttatcatat tggcgatagc
     4921 atttctttct gcacacattc ccaatgtaca ggctgtatca atacacacgc cagtataaac
     4981 atttccgctg tcggtaagga ttgctgcggc aaccccacca gcatcgataa aaggtgaaat
     5041 tgtgcgtgga tttaattttc ccctagcatt attataaagt tccttccatt tcatcaggtt
     5101 gatcctcctg ttgtatccat ataaataatc ttttatacgc cttttccgcc taaggcacac
     5161 aacacattac tcgctttttt cattctaata ttcgtgcgtg aaaatgtcaa acggtttact
     5221 tcgtttcatt acttcatact gctgttcata accctgtttg ttcacttggg caccattcga
     5281 gcaccaattt acccgtttca gggttgtagc gaacacagca gaaatgctca tttcatgggc
     5341 ttttctggct agaaaaaaaa ttttatcgct gccagatatt tactattttt tattaagttg
     5401 ctggagttcc accaccatca ggaggagtag tagagccacc accattattt ccaccattat
     5461 caggaggagt agtagagcca ccaccattgt ttccgccatt atcaggagga gtagtagagc
     5521 caccaccatt gtttccgcca ttatcaggag gagtagtaga gccaccacca ttgtttccgc
     5581 cattatcagg aggagtagaa ccgccaccat tatcacctgt gttattattg ttattattat
     5641 tttcttctgg ttggttattg tctggttttt ttgtatcttc agatgatggt tgagaactta
     5701 ctttagtagg agcagtacct tcaataaatg ctgctttaga gccagaagat tgctttcctc
     5761 catcctctaa attaattttt gtaaaatata catttttagg aacttcaaat tcagtaaccg
     5821 ttaaattagc atgtattttt tccataattt tttcccatag ttttgcagta ctagtacttc
     5881 catgtggtac ttctctacgt tttatagtgt taccagaatc atcttttcta ccagcatcat
     5941 ctccaatata agttgcacca acataataag gagtgtaacc gacaaaccaa gcatcatatg
     6001 cactatttgt agtacctgtt ttacctgcta caggcattcc ctttggaagt gtagcaacac
     6061 caccagtacc ttcagtaatt acgctttcta acatatctgt aacaacataa gctacttcag
     6121 ggtctacaac tttatgttct tcaggagcat ttttaactaa taattgacca tcaaaggtag
     6181 ttatagtagt aaaaatagaa ggttctacat aaacaccttg atttgccaat gtaccataag
     6241 cagcagccat ttgaagtgga gtaataccat tcgccatacc acctagtgtc aatgatggga
     6301 attttctatc acttgcttca gatgaacctg agtgattatt ctttaaagtt gttataccaa
     6361 agttctttaa gtaatccatc attatatcta cacattcatc ataagagtct ccaagcattt
     6421 cagcagtttt gacagctatc gtgtttgaag atttagcaag tgctttacgt aaactcatag
     6481 aaccatgacc agcagtagta gttcttggat tccatttatt attttcttca aacttatatc
     6541 ctcctcgaga atcacttaga gcagttgatt gtgttatttt tagggtatca atagcaggtg
     6601 tatatattgc taaaggtttt atagaagaac ctggctgatg agggttagta gcacgattca
     6661 aagtttttct accacttata tttcttcctc cagctagtgc tcttatttca ccatttttat
     6721 aatctaagat taccattgca gcttgtggtt ggctaactcc atcagggccg accatatgtc
     6781 ctgggaaatt gctatttcta tcaaattctt cttctaaagc atcttgcatt ttagggtcta
     6841 tagttgtgtt tacaattaga ccaccattgt agaataagtt ttgagcttca tcatttgtat
     6901 gtccttgttc cattaaagca tttataactt cactttcgat aaggtcttct acagatgaag
     6961 aaactttatc tgatgattga ggcaatttta tttctatttt agctttgatt gcttcatcat
     7021 attcactttg ttttatatat ttcaattcta acattttctt taaaacaatt tcttgtcttt
     7081 ttttagcttc agggttactt acagcctttc taacaactaa tgtacctgct tttaattgtc
     7141 tataagtatc attagatata agctcccaag attttatttt ttcaatcata tcaaagtcca
     7201 cttgagtagg gtcatctaaa tcatcatcag ttgtgttaac aaaaaataat aatttatttt
     7261 ctaaatcctc cttagtttca tttccctcta acttagaagt tttataggct gaaaatcttg
     7321 atggattttt agttgaacca gcaagaagtg ctgactctgc tagagtgagg tcagctgcag
     7381 acttatcaaa ataaccacgt gcaccagcct cagcacctgc tagacctcta ccaacgaaga
     7441 agttatttaa ataagtttct agtattttat ctttacttac tgttttactc atctcatgtg
     7501 catagtaaat atcctttatt ttacgaggta tagtttgctg ttcagttgtt aacagcattt
     7561 tggatacctg cataggaata gtactaccac cttgcttagt acctgtaagg gtctttaata
     7621 ctgacctaaa taaaccttta atatcgacac ccttatgctc ataaaaacgc tcatcttcta
     7681 ttgcaattac agcatgttga agataagggg aaatcttatc taatggaacg ggtgtctttt
     7741 tatttacact tggagctgta gacaatgttt ttccattagc gtatttaata gtagttgttt
     7801 ggttcgtctg tttatctaaa acagcttctg taactggagt tacatctctt agtgacgcaa
     7861 atactagacc agcagtacct gctgtaccaa caactagtaa aactaaaaat actatcccaa
     7921 atactcttag ttttttaaat ttatcgcttt ttttagattt tttcttgttt tttgttgaac
     7981 gagtcttgtt agctgaagtt ctattaattg gtttatttgt attaccagat gagctaactt
     8041 tttttctcct gattttattt ccatttttat cattatcgtt attcataaga ttttaacctc
     8101 cttacttaat gcaatattat aacacaaata ggagtagttt tgtaattaat agaaaaaatg
     8161 aatttgctat aatattctgt acattgtata tgtataaacg taatatatta aaaagtctat
     8221 atttttattt tatttatttt tgacaaatat tataaaaatt atgccaaagc tacatataaa
     8281 taaataggag gggataattt tgaatagaat agtagataga aaaacaaaaa atgaatttag
     8341 aaaaatcata gctatttttt tagtaattct tttcttgtct gtatttatag gcagttttat
     8401 atatatagat aaaactttaa gaccgacaat aacagtttta gcagagacta aagctcttga
     8461 actagcaaat aggtcgataa ataaagctgt tgctgagatg gtagaaggaa aaataaatta
     8521 tgaagattta atggatatac aacttgataa taatggtaaa ataactatga tacaagcaaa
     8581 cactataatg atgaatgaaa ttgcatctgc aatagcttta gaaatacaag atgaattaaa
     8641 aaaggataag acagcaagtt cttacatacc tataggaaca gcattaggaa gtcctatatt
     8701 agctaaatac ggtcctaaac ttgaagtgtc tattgaacct ataggaacag tatcagtaaa
     8761 ctttaaaacg gaatttgagt cttcaggtat aaatcaaact agacatagaa tatatttaga
     8821 agctcaaaca caagttaaag tagtaatacc acttataact tcaacaaaac aaattaaagc
     8881 tcaaatacct atttgtgaaa ctataatagt aggtgatgtt ccagaaagtt atgtaaatat
     8941 tccagaaaaa aatctaggaa atgttttacc taacactgga aagaatacca acaagtaata
     9001 taaagttgta tatttttaaa taaattcaaa ataatagttt ataaataaaa tttttggaaa
     9061 taatattaat ataattttaa taaaaattta tattttatac tatgtaaaat atctaatata
     9121 tttggtctgt aggttaaata aaagtacctt tagaccaaat atattattaa taacttaata
     9181 acatcttaaa acttatttat ttatattatt tacaacatcc atttaataaa tacaccataa
     9241 cctacagctg ggtcattttc aattgcatgt gacactgctc cgattatttt attatcttga
     9301 actattggag taccactcat accttgtaca ataccaccag tttgagctaa aagcttttta
     9361 tctgtaattt taattttaaa acttttactt tctggatgtt tttggttttc tatatctata
     9421 atttcaatat tatatttttt acacttatta aagttatctt gtaaaattat ttgagctttt
     9481 cctaatttaa cttcatctaa agatgctact ttatattttt ttaaattgga aatatcaaag
     9541 ttattaattt gacctcttat tccaaaatca gtattttctg taaagctacc aataatattt
     9601 tctcgttttg catttataca tccaacacta cctttataag atttttctat attaaggtgc
     9661 ttagttgtag aaattgaacc tgttttaata ggtatttttc ttgcattacc aacacttatt
     9721 ggatgaccta cagctccaaa ctctgatgta tctggattga tatacgttaa agtagcaaat
     9781 ccagaaagta aattgttaaa actaattttt tctaatatgt gtttggtagt tttcattgtt
     9841 ataagttgtc cacctctatt taataaaata gatacttgtt tttcttctgg taaagcattt
     9901 aaggtattgg caaagtcact atatccatca acttcagaat tatttacctt tactatagca
     9961 tcacccaatc taaatggaga tgcttctcca ggatttctaa ctatgatatt ttttaattct
    10021 gcgtcaattt gaagtacgtt tccaattggt attaaatatt caggttcatt tttttgtaaa
    10081 ttttgtgcat aaattaaatt atttgagaaa aaatataata aaaataaaaa tgttaaaaca
    10141 attgtaaatt ttgacttagc taagttggtt tttttactta aagtattatt tttaaaatga
    10201 aaatttttaa gttgcataaa acacctcttt cttattaaat tattttaata atagaattga
    10261 agttttcttg ctgcttctat attatgttgt gcaagaatta tgttttgatg cttggaaaac
    10321 tgactcaata acaccttagt tttcataaaa ataaataaaa taaatagtgt atatgataaa
    10381 ataaatacat attcatataa ataaaaataa acaataactg tctagcataa gaaaggagca
    10441 aaaaatgaaa aaatatagat tgttagttac ttgtataatt gccatgggaa tcataattat
    10501 aggaacatca aatccagtta gcaattttta caagactaca aatttgatta gttcaggaaa
    10561 tatcacaaat gaaaaagcag ataaagatga aaattcaaac aaaaataata aagataaaaa
    10621 tgaagacaaa tctaattcta aaagtaataa agataagtca tcagaggaca aatcgtcaga
    10681 taaaaaaaat aaaattcaaa aaaataaaaa acttttaatt tgtatagacc cagggcatca
    10741 aggaaaagga gatagcaatt tagaaccagt agctccaggt tcttcctcta aaaaagcgag
    10801 agtatcttca ggaacagagg gtattgctac aaaaaaacca gaatatgttc taaacttaga
    10861 agcatctctt gttttaaaga gtatattaga atctaaaggt tataatgtaa taatgactag
    10921 agagacacat gatgtaaata taagtaattc agagagagca atacttgcta atgataaaaa
    10981 agcagatatg gttgtaagaa tacatgcaga tagtttaaat aattcttcaa aaactggagc
    11041 atctatattg attcctgaaa aagatggaaa atatacagct cctatatatg aagagagtaa
    11101 taaatgtgct gaatttatca aacagaatat ggaacaatca ggtatacaaa tcaatggaat
    11161 cgttcaaaga ggtgatttga ctggatttaa ctggtcaaaa gtaccagccg tattagtaga
    11221 aatgggattt atgagtaact ataatgaaga ccaaatgatg tcaaatccag actaccaaag
    11281 aaaaatgatg cagtgtatag ctgatggatt ggatgcgtat tttaaataag atatgaatag
    11341 agaaaaagtt aggttggtat tattttccta atttttatct ctatttttta ttgtacataa
    11401 tgcaaagaag aagtttattt taggttaaat tgtcgtaaat ataactaaaa atattgggaa
    11461 tacttgattt aagtttaaaa agaatatatt atagtataga atgtgtaaaa tttttttgag
    11521 aggtgttgaa atgctagtag tagaaaatgt gagtcatgga tttggtgcga gaacaatatt
    11581 agaaaatgta tcctttagac ttagaaaagg tgaacatatt gcactagttg gtgctaatgg
    11641 agaaggaaaa tcgagttttt taaatataat aacaaagaag cttatgccag atgctggaaa
    11701 tataaaatgg tcttcaagag ctacagtagg ttacttagac caacatacgg tattaagcaa
    11761 ggaaaaaact ataagagaag tgcttagaga ggcttttaag catatgtttg acttagagca
    11821 agaaatgatt gctatgtatg acaaaatggg agaagctagt gatgatgaaa tgagtaagct
    11881 tcttgaagaa acagcagaga tacaaactat acttgaaaat agtggttttt atatgataga
    11941 tgcaaagata caagaagttg caaatggtct tggtcttgga gaaattggtc ttgataaaga
    12001 tgtaactgac ttaagtggag ggcaaagaac aaaagtactt cttactaaac ttctacttga
    12061 aaatccaact attcttatat tggatgagcc tacaaactat ttagacgaag agcatataac
    12121 atggcttact aagtaccttc aagagtatga gaatagtttt gtcttagttt ctcatgatat
    12181 tgaatttata aataatacat gtaatgtaat atatcatatg gaaaatggag agttaaatag
    12241 atataaagga aattatgatg aatttgtaag acttaatgat ataaagaaac gtcaagaaga
    12301 acaagcttat gataaacaag ttgaagaaag aaaaagacta gaggattttg tagctagaaa
    12361 taaagctaga gtagcaacta gaggaatggc taacagtaga caaaagcaac ttgataagat
    12421 ggaaatatta gaaagaccaa aagaaaaaat aaaacctact tttgctttta aagatgctag
    12481 agcagctagt aaaataattt ttgaaacaga gaatttagtt ttagggtatg atgaagcatt
    12541 gacaaaacca cttaatttcc atttagaaag aggaaaaaag attgccttaa aaggaatgaa
    12601 tggtattggg aaatctactc tattaaaaac tttacttgga ataataaaac catttgaagg
    12661 aaatgttaaa ttaggtgact atttggaagt tggatatttt gaacaagaga gttcaaggga
    12721 aaatagtaat acacctatgg atgaagtttg gagtgaattt ccagggctta caaactttga
    12781 agttagacag gctcttgcaa aatgtggact tacaaatgaa catataacga gtcaaatgag
    12841 agtattaagt ggtggagaag ctgctaaggt tagactttgc aaagtaatgt taaaaaatat
    12901 aaatttctta gtattagatg agcctacaaa tcatttagat gttgaggcaa aagatgaatt
    12961 gaagaaggct atcaaagagt ttaagggaac agttctttta gtatgccatg agccagaatt
    13021 ttattcagaa attgttgatg atgtatggaa tatagaagat tttaccacta aaatcgtgta
    13081 gtgatttaac ttattgctgg ggggatttca tgaaaaagtt tctaaatgta cttttttcag
    13141 tttgtattag tattgttata atagtaggtg ttattaattt tactgttgga tttaaacaac
    13201 tttattattt tgacattgat tacttaaata tatcagagtt atctggttta tctgaagatg
    13261 atataaaatt gaactacgat tatctaattg attataactt aaataagaat gtgtctgagt
    13321 tcaagttacc aacattaaaa tcttctccac aagggaagat acattttgaa gaagtcagga
    13381 atatatttca aaatataaat aaattagcta aattattatt ggtagttttt ttagtaggaa
    13441 ttatattgag tgtaaaaaat aaaaatataa aaatcttgaa aactacatca ataacactta
    13501 taattatgcc attattatta acagttccaa ttttattaaa ttttgaaaaa tcatttatta
    13561 tttttcataa attattattt agaaatgatt attggatatt taatccaaat ttagacccag
    13621 ttataaatat attgccagag gaattttttt ttcactcagg aatgatgatt ttaatattaa
    13681 ttttgttagc aagtatctta ctatttgtaa tgtatagatt atataaatct ttcaaaataa
    13741 aataagatag caaaaacgaa ttatattaaa ggttatacaa taaaaatagc tatgtaataa
    13801 tatttaaact aagagtagtc gataaatatt tatgcatagc tatttctaat agaaattact
    13861 ttttactagt gattttaact aaatcttgaa atttgcccat aatgtaattg taattatctt
    13921 ttttgtgagg taacatgcaa ctactgcaat ctttgatacc tttatctgta tacttaaagt
    13981 ttccaccaca gttttctcct aaagcataca aaggacaata gcaaaataga cagttaaact
    14041 cttctggttt attagtttta tgacaaggga aaaattcgca atctttgtga ttaaaaaact
    14101 tataattttc gcccatattt tcctcctaat taaataaaat ttaaattttt tatactatat
    14161 aagattttgg tgataatata attatactaa ataattttat gaatagtcga taatatagta
    14221 ttattaatca tagtattaat ggtataatta gtcaacagaa aaataatgta aaaattatgt
    14281 aagtgtattt tgtaattaga atatgtttta taacaaaaaa tgaggtgatt aagtgaaaga
    14341 aattcaagaa aaagcacttc ttgtaggtct gaatttgact acaatagtga aaaaaaatga
    14401 tgatatagac actagtgaat ctatggaaga attaaaagag ctaacaaaag ctgctggggc
    14461 agaagttgta ggtagcttaa ttcaaaataa acattctgta gatgcagctt attatatagg
    14521 aaaaggtaaa gtagaagaaa taagagccta tagtgactct ttagatgcta ctttagtaat
    14581 ttttaatgat gaattatctg gtgctcaaat cagaaatata gagaatgttg ttggaagaaa
    14641 agtaatagat agaactactt taattttaga tatatttgct caaagagcac ttagtaaaga
    14701 aggtaaatta caagttgaat tagcacaatt aaaatataga ttgccacgac tttatggaat
    14761 gggtggtgag atgagtagaa caggtgcagg gattggtact agaggtcctg gagaacaaaa
    14821 actagaaata gataagagac atatacttaa taaagcagca gacataaggc gtgaacttaa
    14881 agaagtaaaa aagaataggg agacacagag agttaagcgt ttaaagtcaa atataccgat
    14941 tgtagcactt gttgggtata caaatgcagg aaaatctact cttttgaacg aattaataaa
    15001 gacacacaag gattatgaac aagaaaaaga agtttttgta aaagatatgc tttttgcaac
    15061 tttggatgta actctaagaa aggctctttt gccgaataaa aaagaatttt tagtagtaga
    15121 tactgttgga tttgtaagta aacttcccca tgatttagta gaggcgttta aagcaacatt
    15181 ggaagaagta cagtatgcag atttaatttt acatgtaatt gatgcgacaa atactagtta
    15241 tgaattgcaa aaaagtacga ctgaaggtgt attaaaagaa cttggtgtaa atgataaaaa
    15301 acatatactt gtatataata aagtggataa gctagaattg gatatatatc ctaaaagtca
    15361 agaagatatt gtgtatattt ctgcaaaaca aggcataaat atggataagc tattaaatat
    15421 gatagaaatt gctttaatgg aaaatactta ttcagtaagc ttgatgttac cttatgagag
    15481 aggtgatata tttagtagaa taaaagataa atataatgtg gagaattttg agtatgggga
    15541 aaatggcata acgctagatg taaacttcga tgaagaagat tttaatatct atagagagta
    15601 catactagaa aaataggttg aatatggata taaattggga aaatatagat aatcttgaag
    15661 attattttat aacctatctt ctttataaag aatctaaaac agtatctcaa attagtaaaa
    15721 ttagaaatat ttcaagtact gaagcaggtg agcaacttat acaagcaaaa ttaaaaataa
    15781 aggaaatgca aaaagatgat tttgaggcat ctaaggatat tttagataaa tttttagaac
    15841 tagataaaat taagagactt gattttatgg atagcttaga tgacgaaaaa atggtatact
    15901 ttaagagaaa ggttttcaaa agaatccttg tggaaaaaaa tgctgaagat ttaattgttt
    15961 taatttgggc gacaggtgag ctaaaagatg atagattctt aaagttgctt catcagctca
    16021 caaaccacag acactcagat attagaagaa taacttattc agcaatcaga aagattgaat
    16081 caccaagtag tagagaagta ctgcaaaaag gtctctacga taaaaatgca caaactagac
    16141 agtattgtgc aaaggctctt tcaaaactag gggatgaaaa cagtttaaag attttacagc
    16201 agttgaaaga caagaataag aattttgaaa aagaatatgt gcttagagct tatgacgaag
    16261 ccattaagtc attggaaaat gcaaaaaatt tgaaaaagta attttttatt aaagcaattt
    16321 gtttacttaa tgatgtattt ctagagttta acaaggtaat aaataaatta atgggagggg
    16381 tttcaaatga tagtaagacg ttgtgcaggt ggagtagtgt tttatgctaa taaagtactt
    16441 attgtaaaaa atgatagggg agaatggaca cttccaaaag gaaagatact tggaggagga
    16501 cttccatatg agagtgctgt tcaaagagtt aaagtagaaa ctggcataga tgctaaaatg
    16561 atagatgtgg ctggcgatac tatgtatgaa tttttctcaa gaagtagaca acaagaagtt
    16621 tgtaatgcaa taatgtggta tgttatggaa gcttgtaaca ctaattgtgt acttgctcct
    16681 gaatttcaag aaggtggatt ttataaagta aaggatgctt tagatatgtt atcacatcat
    16741 aaagagcaag ctttagtaga agtatcttat aaaaagttta aggaattaaa gaaattagtt
    16801 tcagagggcg atgatgttcc agcaaattaa ttgtgaattt agaggattct gaggaaggga
    16861 tagccccttc cttttttaat tgaagcagtt ttaagcgtaa attatatagt ctctttaaat
    16921 tatgatttat caatttagag ctttagtagt ttatatttaa aatcagtaag atatatttaa
    16981 aataaaaatt aaaaagtata caattttatt atataattgt cttatgatat tgattaaaga
    17041 agtattaaaa gtccatttat ttttaaatag aataatttta tagatatata atttgatgtg
    17101 tatattagaa aatgattaaa ataggaggaa atacaatgag caattacagg acattacatg
    17161 aatttggaac agatgaaata actattgaaa agtctgtttt tattggatat gctaagccta
    17221 taaagagtga agaagaagca ttagagttta taaatgaaat aaagaaaaag cataaagatg
    17281 caactcacaa tgtgtgggct tatactgtag gtaaaaatat gaatatacaa agatatagtg
    17341 atgatggaga accacaagga acagctggaa tacctacatt agaggttata aaaaaagaag
    17401 atttgagaga tgtagtggtt gttgttacta ggtattttgg aggaacaaaa ttaggtgcag
    17461 gcggtctagt tagagcttat acaaaaggtg ctaaattggg acttgaagca ggtaagataa
    17521 tttataaggt aatgtatcaa gaagttaagg ttaagataga ctatactcaa cttggaaaag
    17581 tgcaaaatga gttaatgaat ttggggtatt ttataaaaga tactgtatat gaggataatg
    17641 ttgagatagt agtgtattca agattagaag atgtagagaa attatcagaa aaaatgattg
    17701 atataacaaa tggaacagga aaaataattt taggagagga attttatttg tctgaacaaa
    17761 atggagaaat tttaatataa tctaaaaggt tattagaata gttaatacct caaatagcca
    17821 attagtattt ttaagtgaaa agccattaaa ttgatttaat ctaaatcaat ttaatggctt
    17881 tttatcggat tttgcacaga atcctttttt tacttcaaat aaaccgacta aaagtaaaaa
    17941 tatcccaaat atttttttta atatatctga agacagataa ttagctaata gtgaaccaaa
    18001 tacagctcct aaaaccccaa acacagctat aggtataacc aatttaaaaa ttacattttt
    18061 attttttata tgtatagcaa gtgctataag tgtcataggg atagatgaaa gtaaatttac
    18121 gctttgtgct attttagtgt ctatgccagc aagtaaaacg agtgcaggta taaggattgt
    18181 tcctccaccc attcccattc ctccaataat tcctgcaaaa aatccaatta gagcaaataa
    18241 cattaaaaca ccatccttaa tgctgcaatt atcataatta ttccaaaaga aattctaaga
    18301 aactttccag taaccttatt tagtaattta gctcctataa taccaccaag aatacttcca
    18361 actgcaactt taattgttaa gttaacatca taagtacctt tagatacata taaaacagaa
    18421 cttgctgtcg ttaagaatat tattatagaa agagcagttg catgagattt atgttcttct
    18481 accttaacga tgtcatttaa tattggaact aaaagagtac ctcctccaga accaaaaact
    18541 ccatttataa atcctgtaaa aactccaatt atcgtatttt ttaagttaaa atcaaagtat
    18601 tttttcttta aatcatcagt ttttttcata aaaatcacct tctgaaatag ttttaccaaa
    18661 ataggcaaat tatatttata taaaataaga ttaaatataa tctttcatgt ttgtttaatt
    18721 ttgcataaaa aaaaacatat taaaacaaca tattaatata tggtataata tattaaaaca
    18781 aagtagcaaa tttgagagga gataacaatg tcaaatataa aaatacaaat agacaaaact
    18841 gtagaatggc ttataaataa ggttaatgag gcaaatgcaa aaggattaat tgttggtgta
    18901 tctggaggaa ttgattcggc agtagttgct aatttaatta aaaaggcatt tccagaaaac
    18961 tctatgggag ttataatgag cataaaaagt aatcctcaag atagagaaga tgcacttaag
    19021 gttatagagg gatgtgatat agaatacctt gatttagacc ttatagaacc acaaagtgct
    19081 atattagaca tggtagtagg aaatttaaaa gataaacatt tatatagagc agaatattta
    19141 aagatgacag atgcgaactt aagagcaaga gtaagaatga gtacaattta tacaattgct
    19201 aacaatctag gatatttagt tgttggtaca gacaatgctg cagaaataca cacaggatat
    19261 tttactaaat ttggtgatgg tggagttgat attttaccta ttgcaaattt aactaaagga
    19321 gaagtatatg aatgggcaaa agagcttggt gttcatgaag atttaataaa caaagctcct
    19381 tcagcaggtc tttgggaagg tcaaacagat gaagatgaaa tgggaactac ttacaatatg
    19441 atagatgctg tgttagaagg tagattggat gaagttccta aaagagacca agagataata
    19501 gaaagacttc atagattaag tgaacacaag agaaaaacgc ctgcacaacc tcctaagttc
    19561 taatttacta tttataagat ttgtgataaa atttacaagt gatgaaaatt agaataatta
    19621 attgtacgta ttaaggagtg gaaatatggg acgtataggt aatataatta atagaaaagg
    19681 taaacaagat gctcaaagag caaagatatt tactaaacat gctagagcaa tagcagttgc
    19741 agctaaagaa ggtggagcag acccagagta caatgcagcg ttaaaaacag ctatagaaaa
    19801 agctaaggca gataatatgc caaatgataa tatagataga gctatcgcta aaggtgctgg
    19861 agcaggcgct ggagaagact atgaaactat agtttatgaa ggatatggac ctggtggagt
    19921 tgctgttata gtagaaactt taactgacaa caaaaacaga actgctggta acgtaagata
    19981 ctattttgac aaaaacggag gaaacttagg aactagtgga tgtgtgtctt ttatgtttga
    20041 taaaaaagga caaatattag ttggtttagg agatggagtt tcagaagaag aattgatgga
    20101 tgttgcatta gaagcaggag ctgaagattt cataacagaa gaagatggtt atgaaataat
    20161 aactacacca gaagattttt ctagtgttcg tgatgagtta aaagctaaag gatatgagtt
    20221 tatatctgct gatgtgaaga tgatacctca aactacgact gtattgactg aagaatctca
    20281 tttaaaaatg atgaataaat tagttgatat gcttgaagaa gatgatgatg ttcaagatat
    20341 atatcataac tgggaagtag aataaatagt ataagcatat tgaaatttat aagcattgaa
    20401 tcaattttaa catgtgataa attttataga ataagaaatt actaaaatag acatacgcac
    20461 ctctaaccta tatttgcata ataatatgta agtataggta aggggtgttt ttatatttat
    20521 aggttaagaa atttaaagta aattttgagc tggacttgat tgtctataaa taattgatgg
    20581 gtatataact tgttgctgaa taccatttat acgcccagtt actgtatcaa taatcatcct
    20641 aagactacgt tctgctgtct cttccatttt taaatctata actgtcaaag gaggaatact
    20701 tcttgccatt agtcgtgaag gtccattact tgctgcaaat atttgaatat cctctccaat
    20761 tacaattcca gattcatgaa atgcattcat cataccaaga gctacaacat catgattaca
    20821 aaaaattact ttttttagtg aattttttgc aagcattttt tgagcaatat catatccact
    20881 atctatttta ttagaacaat aatatataga atctgataga tgtattccat gttctttaca
    20941 tgtttttaaa aaagatttac cccttaaagt catacatgta aaggaagttg ggttgaatac
    21001 aacagcaata tcatttttac cattttgaat agcatgtaaa gctgctatac gaccagtttc
    21061 ctcattatca atagatacac ttgaaaaacc aggtatttca cgatttacaa caactacagg
    21121 aattttaggt ttatttttta ctagatattc tatatcatca gcattgatac ctgttaaaac
    21181 aattccatca taataattat ttgcaaaaaa gtattcatca tcacatagat gaccaacttt
    21241 gtaaggtcgt atgatgatat ttatgtttaa atgttctaaa gaaattatgt ggtttattgc
    21301 ttgaagtata ttggttgatg ccaactcaaa tccctcatcc tgccaataaa aagctatttt
    21361 aatattatta tgtttaatag gagtagagcg tttatatcca agcttatcag ctgaatttaa
    21421 tataagctct tgtgtatgta gcggaatcct gcgttcattt cctttaccac ttaagacgta
    21481 cgagacagtt gctgaagaaa gaccacagtc ttttgcaata tctttgattg ttgccatgat
    21541 aaaatcctcc ttaaatagtt gcttaaattt gcttatacca ataatcttaa catatattca
    21601 actaaagaac aatattctac aaggtgcata ttttacagga ttagaataaa taaatttaaa
    21661 aaaacttctt acttatgttg acataattta gtttatagga ttataatatg cttaaataag
    21721 cttattatta actcaaatta agcaaagtta agcaggaaca tgcaatgcta gcttgcaatg
    21781 tgactgtaaa gggggaaaag gtatgagtaa ggtaaatata tttgaagttg gtcctagaga
    21841 tggatttcaa aacctgaaag agtttttaga aactgataaa aagcttaaga ttattgatgg
    21901 tcttattgct tcagggatta aacaacttga aattagttca tttgtaagcc caaaagcaat
    21961 acctcagatg aaagatgcaa aagaagtagc aacttattgt gtagaaaaat atcctgatgt
    22021 gaaactttat gcattagttc caaatttata tggtgctaaa gttgcatggg agtgtggtat
    22081 aagagatata tcctatgtta tatctttgag tgaaactcat aataaaaaaa atataaatcg
    22141 tacccatgaa gagtctattg atgagcttaa aaaaataatg aatacttatc cagacatgaa
    22201 tatatgtatt ggaatggcta ctgtatttgg gtgtcctttt gaaggtttac caaaaattga
    22261 agatgccata agatttgcta aacgcctttg ggaaaacggt atcagggaga taaatctagc
    22321 agatacaata gggattgctg accctaagca agtaagagaa actattagtg cattagttga
    22381 agctttacca gagtgcagat ggcaggtaca tattcatgat actagaaata tgggaatggt
    22441 taatactctt gtggctattg aatctggaat tactactgtt cagtctacac ttggaggatt
    22501 aggaggatgc ccatttgctc ctggagcatc tggcaatact gcaactgaag atttagtata
    22561 tatgcttgaa cgtatggggt atgatacagg agttgatttt gttaaacttc ttgaattatc
    22621 aaagtatcag aagagttttg taaatggagt atatagtggt catcatgtaa atataaagtc
    22681 tgatagttgt aattgttagt tctattgatt aatcgattaa aaaaatatgt tgattaaaaa
    22741 aatatgctga ttggtaaaat agatttattg ataaaaaaga taaattataa aatacattat
    22801 ctaataaaac aaataaaaaa attgaggggg attagtatgg atattatagc tttactttta
    22861 tttttaggag ttattattat tgcatttgta cgtaagaata atgcaggtat tttggctcta
    22921 gctattggtg tcatagctgt tagaattttt ggaatggatg ataaagaact ttatggagct
    22981 ataagcaact caatgtttac tacacttgtt ggtataacac tactctttgc aatagtgaac
    23041 tcaacaggag cattaaagct tgttgcagaa aaaattgtag ctgcttctgg taaacgtgtt
    23101 tggcttatac ctatagcagt ttttatagca ggatttgttg ttgctggtgt tggacctggg
    23161 gcaattccag cacttgcaat tatcccagca atggctgttc ctgtagccat aacggttggt
    23221 tataatcctg taatgcttgc acttataggt gaatgtggat taatggcagg tcgtatgact
    23281 cctattactc cagaaggtca aattataaca accgttgcag aaggggttgg aattgaaaat
    23341 gttatgccta ctatcttagc ctgtcaaact ctttctacaa ttgtattttc aatagtattg
    23401 tttataattt tcaaaggata taaacttaaa aaacctataa atgttataga tagtaagcaa
    23461 atagaaaaat ttaatataaa gcagataatt tctctaataa gcatagtagt tatgattgct
    23521 tttatcataa tttttgatat gaatgttggg cttgcagctt ttgcagtttc agctgtactt
    23581 atttttttca atattgctga tgaaggagaa tgtatcaaat caattccatg gagcacgata
    23641 gtaatggttc ttggagtagg tgcaatgatg acaatcgtag atgcagcagg aggtattgac
    23701 ttgatgagta atgcattatc atcactgatg aatagtaaaa cagctactcc aattatgagt
    23761 atttcagcag gacttatgtc tatggttagt tctgctttag cagtagttta tccaactatg
    23821 atgcctatgt gtgttgatat agctaaggca gttggaggag tagacccact agcacttatt
    23881 gctgcagtcg gtgttggagg ttctcttgct ggtgtaagcc cactttctac tggtggggca
    23941 ttgattttag cagctatggg ttcaagtaag aaagacttta ataaggaaga acaaaataag
    24001 gtttttgtac agcttttcat tatgagtgca gttggcttat tggttattgc agtagtatca
    24061 atattatcat ttaatgcaat aacacattta atgaactaat tataataact gaaaataatt
    24121 cattaaattt aatttttaat attaatcaat ataatataat ttggagggga agttatgaaa
    24181 aaaggagcat taagcaatat aaaggtactt gaccttacaa gagttcttgc aggtccatat
    24241 tgtacaatga tgttggcgga ttttggagct gatgttataa aaatagagat gccaagaaaa
    24301 ggtgatgata caagaggttt tggacctttt gtaaatggag agagtttata ctatgcaaat
    24361 ataaatagaa ataaaaaagg tatcactcta aaccttaaat cagaagaagg aaaaaatata
    24421 tttttagaaa tggtaaaaca tgctgatgtt gtagtagaaa attacagacc aggtgtaatg
    24481 gaaaagttag gtctgggtta tgatgagttg gctaaagtta acaatcaaat aatttatgga
    24541 gctgtatcag gatttggatg ttatggacca tattcagaac gtccaggtta tgatattatt
    24601 gcacaggcaa tgggtggtct tatgagtata acagggcaag aaggaggaga accaacgaga
    24661 gttggtaatg ctatgggtga tgtactaggt ggaatgaatt taactattgg tatactcaca
    24721 gcactcaacg ctagaaaaat aataggaaaa ggacaaagag tagatgtttc cctcgttgat
    24781 tctgttgtat cgtctctcga aactggtata cagagatact ttgattcagg acaacctgaa
    24841 cttatgggta atagatatgc ttcagcctat ccttatgact cttttaaggc aagtgatgga
    24901 agttttgtaa taggctgtgg taatgacaaa ctttttgaaa agttatgtaa aaaagttctt
    24961 tttagagaag accttttaat agatgagaga tttaaaacaa atcttaagcg ttgtgaacat
    25021 tacaaggctc ttaaaccaga aatagaaaaa tggtctgtta agcatacaat aaaagaagca
    25081 gttgacttaa tatcagaggc tggagttcct gctgctccta tctataatct tgattgtgtt
    25141 acaacagatg aacatatagc aaaagcaaga gaaatgattg tagatattag tcatcctatt
    25201 ataggaaata tgaaagtcaa tggaaatcca gttaagttga tgacaacaaa agctaaaatt
    25261 gatatgccag caccatctct aggggaaagt aacgaggaga tttataatgg ttggcttggt
    25321 tttgatagtg ataaaataaa agatttaaaa gaaaatggag ttatttgatt aaaaaatagc
    25381 taaacacact tgtgacttta gtcatgggag gttcagttag aataatatca aaaaatatta
    25441 aatcaatact gtaaatcaaa tatagaattg tcagaagcat ttaagatggg aaaagctact
    25501 tagacattga gtctttgtgg cttttcctat tttcattaag aactttatat tatcagaata
    25561 gatatgtact aaactaaagt attaacaatt taaattaata gaaaataaac tacctaatta
    25621 tgttgaataa agactacttt agtgaaaata aacttataga gatgctattt aaattatttt
    25681 aatggaaata tcaaaaatga gactgcattc tcaaaaatga gactgcgttt tttaagtatt
    25741 ttctcgaaaa tgagacaaaa atattgtttt atgagtttta ttgaagaaaa attcttctgt
    25801 gaattacaat ttaatcaatt ttaaagttga aaattattaa aaatttagtg aaatttatgt
    25861 atatttcgtt tattttatta aaaataacaa ttttttacta aaaataaaga acttcaatgg
    25921 tgattggcat agtacttgct attttatatt aacaagaaat gtagaaaaaa taacaatgta
    25981 taatgggggg aatatgatgg aatctgtaat ggaaaatttg aacaatctta aggtggaggt
    26041 taaggacaaa gtctgtgtta ttactataaa tagacctaaa gctttaaatg ctcttaatag
    26101 tgacactctt agggaactat cacaagttat tgatgtagta tcagaaaatg aggcaatatt
    26161 aggagttatt attactggag aaggtaaagt ttttgtagcc ggtgctgata tacgccagat
    26221 gcaaaactat aaaagtgagg aaggcagaaa atatgcaggt tatgctcagg gaatttttga
    26281 taaaattgaa gctcttgaga aaacagtaat agcagcagtt aatggatatg cattaggtgg
    26341 tggatgtgaa cttgctatga gttgtgatat tagaattgca tcagaaaagg ctatatttgg
    26401 acaacctgaa gttaatttag gagttattcc atgttttggt ggaactcaaa gattatcaag
    26461 acttgttgga actggaatag ctaaagagtt aatttttacg gggagacaag taaatgcaga
    26521 agaagctaaa tctattgggc ttattaataa agtggttcca agtgatttac ttttagaaga
    26581 gtctatgaag atgatgaacc aaatagttga aaaagcgcca atagcaataa gatatgcaaa
    26641 agttgttatt aacaaaggga tagatatgga tttaaagaat gctcttgaac tagagaaaga
    26701 tattgcaggt ttaacttttg ctacaagaga taagcaagaa ggaatgaatg catttataga
    26761 aaagagaaag ccagtttttg aaaataagta gctattataa ctaaattgga acaatgtaga
    26821 atatattcaa aatctaaatt tcgtgaaaaa atacatatag tttaatttta aaaggagtta
    26881 atttatgaat acgggagtaa ttataagttt agtagggatt atcatagctc tatctcttct
    26941 agttgtaatg gttatgaagg gaataaatat ttttattata gcaattgtat gttcatcatt
    27001 agttgctata acaggaagcc taaatgtgta tgatgcactt aaagtggatt atatgacagg
    27061 atttgttgga tttcttcagg caaatttctt tattttccta acagggacat taatgggaaa
    27121 aataatggag attactggtg gagcaaaatc tattgctaag atgattgtaa gatggattgg
    27181 taaggataaa gcactacttt ctattccaat agcttgtgga atattagcct atggtggagt
    27241 aagtgttttc gttgtaagtt ttgcagtgtt tccgatagct ttagaaattt ttaaagaagc
    27301 tgatttgcca cgaagattta taccagcagc tttgactttt ggatgctcaa cttttgctat
    27361 ggttgcacca ggggcaccac aaattcagaa tattgttcca gcatcaacat tagggacaga
    27421 tattatggcc ggagcagtaa atggttttat atcatgtgca gtaatgttta tagttggaag
    27481 taccattttg tacaaaatgg tttcaaaaga aaaagccaat ggtggtcatt ttatagcaca
    27541 tgaatcagat atatttgatg aagcagaagc aactaattct aagagagaaa atggtcctaa
    27601 tggtgtaatt gccttaattc cattgatagt gtcaatttta ataattaatg taaaagttaa
    27661 tggaaaagcg atagtaccaa ttgaggtggg agtatttatt ggagctttat tagtatattt
    27721 attgttaaat aaatatcaag ataatactaa aatagttgga catgttggtg atgcatgtaa
    27781 aactacagta gtagcaatat gtaatacttg tgctgtagtt gcatttggtt cagtagtaaa
    27841 atcagcagtt ggttttgact tcatagttaa tgccatgact aatattcctg gtcctcctat
    27901 tgcaggggct gcagttggaa ctacagttat tgcaggtatt tgtgggtcag cttcaggtgg
    27961 actaggaatt gcagctccac ttcttggacc agtattttta gccaagggtg tttccgctgc
    28021 agcattaact aggactatgg caatttcatc agcagcatta gattcacttc cacacaatgg
    28081 atatattgtt actgtaacaa atggtctgtg taaagaaaca cataaggatg cctatatgcc
    28141 aatattctgg gtaactgttt taactccgtt tattggaacc atagtagcag tcctactgtt
    28201 tacaatgttc ccgggattgc cataagagaa aagataaaag gtataaatta tattttgata
    28261 ttataaaatc aattttcaaa atatatacaa taaatttcat gttttaatac tataaatatg
    28321 tatttaaaat aacaaattga ccatataagg aggaattgaa agtgttaaat aaaccattag
    28381 agggagttaa ggttattgac ctaacgtatt ttgtagcagg tccagggact tctaaaatat
    28441 tagctgattg gggtgctgac gttattaagg tagagccaag ctttggagac ccaggaagaa
    28501 agacaggggc aacaatgaca atgccaatag atgattataa taatcctttt tacagcacat
    28561 ataattcaaa taaaagaggt ttgtctataa atcttaagag tgaaactgga attgaaatta
    28621 tggataagct tttaagtgaa gcaaacgtat ttgtttcaag ttatagaaca ggagcattaa
    28681 aaagactagg tcttgattat gaatcattaa gtaaaaagca tccacacctt atctggggtc
    28741 agataaatgg ttttggcgat tttggacctg ctaaggataa tgcaggattt gatacagttg
    28801 ccttttgggc aagaagtggg gctatgcttg atatagcaga aaaagataca agtccaataa
    28861 atccacctat agcctttgga gatgcaacaa cttcttgtag tttatctgga ggaatatgtg
    28921 cagctttgta ccagcaagct aagactggaa aaggacaaaa agtaatggca tctttatttg
    28981 ggcaagcaat atggaatgca tcatctttat tagcatctac acagtttagt gatgagtatc
    29041 caaaaactag aaaaaatgct atttcaccag taataaattc ttatcgttgt aaggatggaa
    29101 agtggatatt cttaagtatt ttagagcatg aaagatattt tgaggcactt tgtagattat
    29161 ttggaagaga agacttaata aaagaagaga aatttgcatc ttcagtagaa agtaagaaaa
    29221 atgctacaga acttattaat attttggatg gtgaatttgc aaagtttact caaaatgaga
    29281 tggttgaaaa acttactgct gctgatatag ctcatgaaaa aattcaacat gttaatgatg
    29341 tagtgactga tgaacaggct attgctaata actatatata tgagcataca agtaaaaatg
    29401 gaaataagac aatgcttgca tccactccag ttaaatttgg aaatattgaa gttaacatga
    29461 cttgtgatgc tccacttata ggagaacatt cagatgagat attgagagaa cttggataca
    29521 gtgattctga tatagaagct ctaattgaat ctggaattgt aacaatgaat aaagaagcat
    29581 gtgaaatatg ttaataaaca tgtgaagtat gttaataaat aggagggaat tttatggatt
    29641 ttagattaac agaagcacaa ttaatgcttc aaagagtagc aaaagaattt gcagagaatg
    29701 aaatagcacc aatagctgct gaaacagata aaacaggtat ttttccaagg gagcttttta
    29761 gcaaaatggc taaaatagga tttaatggca ttggaacacc tgtagagtat agtggttctg
    29821 gagggacaga tatagaaaag gtaattgttg ttactgagat tgccaaaaaa tgtgcagcat
    29881 ctgcagcaat attatcaata cacacaatat atgctcaagc tattttgaaa tttggtactg
    29941 aggaacaaaa gaaaaaatac cttccaatga tggctgaagg tgggtgtgta ggtgctttcg
    30001 ccttaactga acctaatgct ggttcagatg ctgcaagagc tgcaacaact gcaattattg
    30061 atgaagaaac ggatgaatat gttttaaatg gaactaagtg ttttatttct ggtggaggtc
    30121 aagcagaatc attaataata tttgcactga cagacccatc aaagggaata aagggaatgt
    30181 ctgctatcat tgtcgacaaa ggtactccag gtttttcaat aggaaaaatt gaagaaaaaa
    30241 tgggtattca tggttcagaa actgcagagt taatatttga taactgtaga gttccaaagt
    30301 ctaacttatt aggaaaagaa ggcaaaggat ttaatatagc tatgacttgt ttagatggag
    30361 caagaattgg aattggagct caagctgtag gaattgctga aggggcttta gaagaaagta
    30421 tcaaatattc taaagaaaga gtccagtttg gcaagccaat ttcagcgttg caaggtattc
    30481 aatggtatat agcagatatg gcgacaatgg tggaatcagc aaaattatta gtatattatg
    30541 ctgcagattt gaaagctaga ggagaaaaac acactaaaga agctgctatg gcaaaataca
    30601 atgcgtcaag tactgcaaga gaggttacaa atctagccct tcaaatccat ggtggatatg
    30661 gatatatgaa ggattatcca ttagagagaa tgtacagaga tgccaaaata acagaaattt
    30721 acgaaggtac ttcagaaata cacaaagttg ttatttcaag agcagtacta ggataggagg
    30781 aaaagaatga atatagttgt ttgcttaaag caagttccag atactaatga agttaaaata
    30841 aataaagaga caggtactct aataagagat ggtgtaccaa gtataatcaa tccagatgat
    30901 agaaatgctt tagaagaagc tctaaaaatg aaagatgaac taggagcagt gattaaggtt
    30961 ataagcatgg gacctcctca agctaaaagt gctttaaaag aagctttggc aatgggagct
    31021 gatgaagctt atttaatctc agatagagct tttggtggtt cagatacttg ggctacatct
    31081 acaattattg ctgctgctat agaaaaggtt ggaaagtatg atgtaatatt ctgcggaaga
    31141 caagctatag atggagatac agctcaggtt ggaccagagg ttgcagaatt tttagggata
    31201 ccacaagtta cttatgcaaa agaagtaaag gttcaagatg acaaattact agtaactcgt
    31261 tatacagaga caggggatta tttaatagaa gcaaagatgc cagttttatt aactgctata
    31321 aaagagttaa acaatccaag atatccaagt gtaaaaggaa tcttagaagc ttataataat
    31381 ggagatgcga agattactgt attaacactt gcagatttag atgttgatac aactcaaatt
    31441 ggattaaagg gttcacctac aaatgtttat aagtcttttg caccagttaa agacaagcat
    31501 aatgaaataa tagaaggtat aaataaaaaa gaaaaagctg agaaactaat agaaatacta
    31561 ttcgatttga aactagtata ggagtgttaa gatgatgaga gcaaaagtta atcaaggtat
    31621 aaatttaaat gattataatg gtgtttgggt aataggggag caaagggaag gcaaaattaa
    31681 tcctgttacg atagaactta ttggtgaagg aagaaagctt gctgaccaat taggaaaaga
    31741 gcttgaagtt gtgatagctg gatatgaagt agagaaagaa gtgaaggaat tacttcacta
    31801 tagtgttgat aaagtatatt acatcaatga ccctcttctt aaagatttca ctactgatgg
    31861 atatgcaatt tcaattgcaa atttaatcga aagaaaaaag ccagaggttg tattggttgg
    31921 tgctacttct ataggaagag atatagcgcc tagaattgca ggtaaggttg gaacaggtct
    31981 aactgctgac tgtacaaaac ttgaaataga ttcaacagat aataagttat tacaaacaag
    32041 accagcattt ggtggaaatt taatggccac tattgtttgt ccaaaaaata gaccacagat
    32101 gtcaacagtt cgtccaggag ttatggcaaa agctgtaaga aacgaatcag agactggaat
    32161 tttagaagtt gttactccag aacttactga aaaaatgatt agaactagat tagtagaggt
    32221 acttccacaa gaaaagaaat ctgtaaatct aactgatgct agaataatag tatcaggagg
    32281 aagaggatta aaaagagcag aaggatttga gcttataaaa gaattggctg ataaattggg
    32341 ggcagagatt ggtgcttcaa gagcagcagt agattcaggg tggatagaac attctcatca
    32401 agtgggacaa acaggaacta ctgttagacc agaactatac attgcatgtg gaatatctgg
    32461 agcaatacaa cacttagcag gtatgagtga ttctaaatat atagttgcta taaataaaga
    32521 tgcaaaagcc cctattttta gtatatgtga ctatggaata gtaggagatt tatatgaaat
    32581 tatacctgaa atgatagaat cattaaatag gtaactttta gaaaacattg aatatgtaga
    32641 atgataagtc ttataatatt gtattgtaag acttattatt tttgctgaag gttatagtgg
    32701 ggagatgaaa caatgaaaga gcagtggtat aaggatatat ttgcaagaat attatctatg
    32761 actgatgatg gttttattgt tgtaaataca agtggagtaa taatagatat taatgataaa
    32821 tattgtgact ttttaggaaa agaaagaaaa gatataatag gacaaaatat acaaagtatt
    32881 ataccaaata caaaaatgct agatgttatg aaagataagt actgtgaaga aggagctata
    32941 catcattatt caggtggaaa tacaaaagaa aaaagtgtaa tagtaagtag gtcttatgta
    33001 gaaaatgaca atggagaagt agttgcagga gtagctcaag ttaaatttag attgcaatca
    33061 tttgatgtag ctaaaaaatt aatgtcagaa tatatggagc tccaatatta taaagagcag
    33121 tttaaggata actgcggttt tgacaagctc ataggtgaaa atagagactt catagaactt
    33181 aagaaaacag gtgtgaaagc ttctaaaaca aactttccag tacttttaac gggtgaaaca
    33241 ggcacaggta aagaagtctt tgctagagca attcacaata atagcagtag gtcagataaa
    33301 cctatggtta gtataaactg cgctgcaata ccagaagaat tattagagtc agagctattt
    33361 ggttatgacg aaggagcttt tacaggagca aaaaagggtg gtaaaaaagg taagttttta
    33421 gttgcaaata atggtacaat ctttttagac gagataggtg acatgccttt gaccatgcaa
    33481 gcaaaacttt taagggttct tcaggaaagt gagatagaac cagtgggagg tcttaaaact
    33541 ataaagatag atgtaagagt aatctctgcc acaagaaaga atttaagtaa aatggttgaa
    33601 gagggattat ttagagaaga cctatactat agattaaatg taataaacat tcatatgatg
    33661 gaattgaagg acaggcaaga tgatatttta ttgctagcaa attatatatt aaacaaatta
    33721 aatgttgaat acaaggaatt gaaggtttta agtgataagg ttaaaaattg ttttattaac
    33781 tatacttggc cgggaaatat aagagaacta caaaatgtaa taaaaagtgc ctatgctgta
    33841 agtgatgata tggtcattat gatgtgtgac cttccatcta agatggataa tatcagcaga
    33901 gtagctcaat gtaatgtgga tagcaattgt tcaattcatg aaatggttga gaattatgaa
    33961 aaaagcttaa ttattgatgt gttaaggaag tataattgga aatgttctaa ggcagcagag
    34021 gttatgggaa tacacaagag tcttttatat aagaaaataa aaaagtatga aattgaatta
    34081 aataattaag ataacttgaa ttaaggcaat gctaaaaaaa gcgcccttat tcaaagcgaa
    34141 ctgtataaac tgttttatgc agttaaaacg tgtaaaagta gatgtgaagt actaatgtca
    34201 atcattagta ctttttattg tatttataat tactatctat atttcagtat cttttgatat
    34261 ctaagattat tatgtttaat ttaggatata acaaaaccct cttagttatt gacagttttt
    34321 tatattttaa ctgtctacta agaggggata gtttagttta aatttagagt aaattttatc
    34381 tagcatttat aaaaaatatt ttgaatagct tttttgttat ttagtcataa ttttctactt
    34441 ctttatcaag ttctttgcca caagaatcac acttatatat agcattgtat ccataatcat
    34501 attcatcaac tattttcttt tgtctaaata caccaccaca ctcacatagg aaaccaattt
    34561 ttataagtgt aacgataaat tgtgatttta taaattttat atcattacat ttatcgttag
    34621 tgtaaaatgt ataaatataa gagttatcgc actcatctct taattcaaat ctttcaatat
    34681 atgtgttatt ttttaattcc tcaaattctt ctttagttaa atatgatttt tgttgcaatt
    34741 catgtaaacg cttaatttgg tccattattt atatcctccc aataatttta gtattttaag
    34801 cttagataag gaatctaagt tttattaaat ttatcccaag gaattattta aatcatattt
    34861 agttttatta attacaacat atataaatta cacgaaaata aaactaattg taaaaataat
    34921 atcctgtgaa aacctaaact actaacccct tactaacccg aaaactaaaa aaagaaaata
    34981 tatccaaata taagtgatat ataaataaag atgttttata taaatttaac tttaaatatt
    35041 ctgacaataa tatatataaa tatattatag ttaaaatata ccacatattt tgggcattgt
    35101 atactggaat tagagtaatt aatttatttt ttaacataca aatgatttta tgaaaaaaaa
    35161 caagactcaa aaatttttat attctttgat ag
//
LOCUS       DAEPOZ010000012        33769 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.012, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000012 DAEPOZ010000000
VERSION     DAEPOZ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33769
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.012"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            246..812
                     /locus_tag="KR440_000504"
     CDS             246..812
                     /locus_tag="KR440_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0697572.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            complement(1031..2017)
                     /locus_tag="KR440_000505"
     CDS             complement(1031..2017)
                     /locus_tag="KR440_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HBH0697573.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSIAGGIDVPLAMGSKSTYTRSKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLNSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(2047..2742)
                     /gene="pxpB"
                     /locus_tag="KR440_000506"
     CDS             complement(2047..2742)
                     /gene="pxpB"
                     /locus_tag="KR440_000506"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBH0697574.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            complement(2762..3982)
                     /locus_tag="KR440_000507"
     CDS             complement(2762..3982)
                     /locus_tag="KR440_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HBH0697575.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVDTMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            complement(4011..4802)
                     /locus_tag="KR440_000508"
     CDS             complement(4011..4802)
                     /locus_tag="KR440_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HBH0697576.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRSVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELEGIEVCPLENIEVCSSENIV"
     gene            5391..6020
                     /locus_tag="KR440_000509"
     CDS             5391..6020
                     /locus_tag="KR440_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0697577.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            complement(6134..7648)
                     /gene="glpK"
                     /locus_tag="KR440_000510"
     CDS             complement(6134..7648)
                     /gene="glpK"
                     /locus_tag="KR440_000510"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBH0697578.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            7891..8433
                     /locus_tag="KR440_000511"
     CDS             7891..8433
                     /locus_tag="KR440_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0697579.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            8447..10943
                     /locus_tag="KR440_000512"
                     /pseudo
     CDS             8447..10943
                     /locus_tag="KR440_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11364..11762
                     /locus_tag="KR440_000513"
     CDS             11364..11762
                     /locus_tag="KR440_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0697580.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMMILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(11979..12188)
                     /locus_tag="KR440_000514"
     CDS             complement(11979..12188)
                     /locus_tag="KR440_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697581.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(12220..12429)
                     /locus_tag="KR440_000515"
     CDS             complement(12220..12429)
                     /locus_tag="KR440_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0697582.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            12728..13147
                     /locus_tag="KR440_000516"
     CDS             12728..13147
                     /locus_tag="KR440_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0697583.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            13375..13830
                     /locus_tag="KR440_000517"
     CDS             13375..13830
                     /locus_tag="KR440_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697584.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(14055..14315)
                     /locus_tag="KR440_000518"
     CDS             complement(14055..14315)
                     /locus_tag="KR440_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697585.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTDIKGMLQNGV"
     gene            complement(14578..14931)
                     /locus_tag="KR440_000519"
     CDS             complement(14578..14931)
                     /locus_tag="KR440_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697586.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQAKQDDDITNNFI
                     ANAEIYEMLLSQSSVNEASNISTFSVRKSGGESGMVEVYVALILRGRKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(14948..20125)
                     /locus_tag="KR440_000520"
     CDS             complement(14948..20125)
                     /locus_tag="KR440_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome condensation regulator"
                     /protein_id="HBH0697587.1"
                     /translation="MKQNKLLQRGAYFNDKNILIDDFDKRYNDYDFVEFFTGISNSTF
                     GLKSDGNLYACGDNTGFQLGLGKDSSERRMFSKVKIDNVKYVSCGSKHSVAVTKDGFA
                     YGAGTSNVGQLGVIESTVYYEFTKLPIDDVKTVACGYDFTFVLKNDGTLYSAGLNSSG
                     QLGLGDTNNRATFTKVNIDSVKDVVTYNQSVFIIKMDGTAHACGLNSNGQLGINSTLN
                     KSVFNKIEGMDNVKQIACGSSHTILIKNDGTMYTTGYNGVGQLGTGNNNNSIVFTLSS
                     INNVKYASCGNNHTMILKYDNTLFSTGQNNYGQLANANKDVASRNTFAKVNVENIKDI
                     KCGSQFNFLINGSKEIFVSGCNLAGQLGSFFHTTFLYEFSNVQSSNLDNYSGLLVNDD
                     YLYVTKDNSEFLNVKLSDNFQDYKKIELTDSNMFIVMNDGTLYACGLNNYGQLGLGDT
                     VNRSVMTKVDIDNVLDIKGNGNSTFVLKNNGTLYSCGLNSNGQLGLRDEVNRNIFTKI
                     EIENVKDFCVGSNYVIALNHSKEVYGWGNNPYNNIEKTSNYPYKQGISNIEKIAAYDY
                     SVYMINSEGKLYVSGYNYNYQLGKGNNSNQSKALVSQCRTNSTSSTSNGLRTLPKITN
                     VFPFYDGCAIIDEGGYVYLTGYHGYLRTLNSSPSISDYSRYGTFIEATNSNHNTYFIQ
                     ETDFSGIEKVIGMSNNILFFKKGSSYITGYPKTFGSTITGHRSYTSINSESSNLGSNF
                     IIYHSNSKLYGKGIANSGQFGNSTNIDGTSNYDTGLKDIKDIIVKGNTVVVVDKNNNI
                     YVTGMNQNNKLGIGEYNNEPVKKFTNITEQSNSFIFMDDIKEITTSRNTMFIVKNDGT
                     AYATGNNSSGQLGLGDTINRNKFTQINLDNIKKISTSIDGNTTFAIRNDGTLYSTGLN
                     TKGQLGLGDIVNRNTFTKVNIQNVRDVVLGTTHSHAIKDDNTLYSCGENTHGQLGLGS
                     ESNHPDVLTFTVNNITNVRDVYCSDTTTFIVKDTNIAYCCGYNNNSQLGMGNTTDQYS
                     FIKCMENVKEVIPNEINTYIITIYNTAYSTGLNTDYCLGLNSNSNQSSFSEIPISNVV
                     KVAPNRNNAVLLLTSEGDVYTAGKCSNGSGTGSETPEKIKKIASKAKDIGMNYRCGHY
                     VSDNGDLYGTGFNNNGQLGVGDVTKRDTFIKTNTRVKKILPLEYANIAIKDTNDIYIC
                     GLNNYGQLGVGNRYDSRNNDNRIFNYKHMNFVMGDLTSIKNRHNFILLNNKIVIPTTK
                     DIDYGLVLGNLYKGDLYTELPYEDIKEVSISKTHIIILLNDGTMYGCGTNYHGELLQD
                     LSINQVDEFVQINVSDVKHVSCGDNFTYFIKSDDSLWSIGKNSEYQLGIGHNNPVTEL
                     QRITTISSCKEVHCGKNYTLVVTTGNELFVQGYNDKGALGLGSDSENTIIKFFTKALT
                     DIREIKSYGSDHILVLKNDNSVWVTGKNRDVYKIEQPVEFLKEFTIVPISEDVNTVKD
                     VLATDNTLYIISEVGTTNAAIEITEKSISSIKIKIQDPNKDISRIEMLINGESVKSVS
                     DLTTEKISFEVPPDKIKIGENKILFRAYCKGDDLYASLFIFKESTGNSIIKDSYVMIG
                     NRMYKVVNTTSNEQDITITLDRGLEEDLNLGDPIYQLINKTKVQVKINKSDLFKDMKL
                     VEIKKSDSSYQEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAENNEPQA"
     gene            complement(20147..21133)
                     /locus_tag="KR440_000521"
     CDS             complement(20147..21133)
                     /locus_tag="KR440_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBH0697588.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKFD
                     KNIKEIKVKIGDTDILTTDSKDLSGAINEVVKKIENISFDDVISGQIQTDISVLKNSY
                     NKLSEKVLDILIYLELESEVTVDEAGYWYDTLANGNNIVAIEGLKLDLNRKCITGEIG
                     NVIFRDVVLPFSANRVRYIHDMDNNFVETKSSNTYLKEQKDITLSKYSYEIR"
     gene            complement(21134..21832)
                     /locus_tag="KR440_000522"
     CDS             complement(21134..21832)
                     /locus_tag="KR440_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBH0697589.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSEMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMLSREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(21829..22881)
                     /locus_tag="KR440_000523"
     CDS             complement(21829..22881)
                     /locus_tag="KR440_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBH0697590.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKIHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGENNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEISISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(22874..23302)
                     /locus_tag="KR440_000524"
     CDS             complement(22874..23302)
                     /locus_tag="KR440_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBH0697591.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDVLSANVKVSTIYGEVEINV"
     gene            complement(23302..23628)
                     /locus_tag="KR440_000525"
     CDS             complement(23302..23628)
                     /locus_tag="KR440_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HBH0697592.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(23644..25173)
                     /locus_tag="KR440_000526"
     CDS             complement(23644..25173)
                     /locus_tag="KR440_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBH0697593.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGTIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLSVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            complement(25455..25877)
                     /locus_tag="KR440_000527"
     CDS             complement(25455..25877)
                     /locus_tag="KR440_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697594.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEVATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(25877..28330)
                     /locus_tag="KR440_000528"
     CDS             complement(25877..28330)
                     /locus_tag="KR440_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697595.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASSRVNSLNSSLRKVQTTASSVSSTMAKLSSSINAVSGVIGSLNGSI
                     MRLAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     IWNIVIKAKDMTKRVISSILGKLKRVEKRPYQGSINLKDMVSSAMARILPKLMLFKNT
                     FWSGVIAIKDMASSIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKTSQKDVDKYYDSLVNMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSSKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(28330..28497)
                     /locus_tag="KR440_000529"
     CDS             complement(28330..28497)
                     /locus_tag="KR440_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697596.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(28524..28970)
                     /locus_tag="KR440_000530"
     CDS             complement(28524..28970)
                     /locus_tag="KR440_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBH0697597.1"
                     /translation="MNENGLSKNINIVDLLLNADTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(29084..29512)
                     /locus_tag="KR440_000531"
     CDS             complement(29084..29512)
                     /locus_tag="KR440_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBH0697598.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(29526..30590)
                     /locus_tag="KR440_000532"
     CDS             complement(29526..30590)
                     /locus_tag="KR440_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBH0697599.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESRVNKGELILIKEAGAIR
                     IARGVNSLTELTEEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(30595..31038)
                     /locus_tag="KR440_000533"
     CDS             complement(30595..31038)
                     /locus_tag="KR440_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697600.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPIMLNTNNKDIVISIKYLPKPQSKSIRMYEISDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTITLNYLDSVYEEDVVYENMEEINLNLGE"
     gene            complement(31287..31727)
                     /locus_tag="KR440_000534"
     CDS             complement(31287..31727)
                     /locus_tag="KR440_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697601.1"
                     /translation="MENKKDILFKETDERLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(31832..32029)
                     /locus_tag="KR440_000535"
     CDS             complement(31832..32029)
                     /locus_tag="KR440_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0697602.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            32198..32518
                     /locus_tag="KR440_000536"
     CDS             32198..32518
                     /locus_tag="KR440_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBH0697603.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            32600..33106
                     /locus_tag="KR440_000537"
     CDS             32600..33106
                     /locus_tag="KR440_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697604.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
BASE COUNT        10983 a         5776 c         3789 g        13221 t
ORIGIN      
        1 ctcttttcca aaatataatg tgataatctt aattcaatta atactcttct attaaatttt
       61 tttattatta ttttctttat attaatttaa caataattca ttttcataga cttttatttg
      121 gatttaatta aattttaatt gtcaaaatct actatgtatt cttattgcca ctacttctat
      181 gttgtgctat aattactttg ttttaagcga atattttata ttaattttat tatttggagg
      241 ttcttatgct aggtgaaaag atgagaaata taagaaaaag caagaaaaaa acattaagtg
      301 atgtttccaa attgacagat ttatctataa gttatatctc tcaaatagaa agagatgcta
      361 ttgaaccatc cttgtcttca ttgagaaaaa tcgctgaagt tttagacact cctttatata
      421 tgtttatgga tgacaataat actgatgatt tagtaattag aaaagaagat agagttatga
      481 tgaaatttcc aaagagtgaa atgttttacg agatagtatc tccaatgcct acaactgaat
      541 ttacaccttc aattttattt gtagagtttg aacttaaacc agaaagtgag gatgctaaag
      601 attatatcta tcatgcatca gaagaaatta tggtggtaac gagtggtgtg gttgatattt
      661 gtatgggtga aaaggtagtt agattaaatc caggtgactc aacatttata aaagctaatg
      721 taccacataa aatcatcaat ccaagtaaag atacaatagc tcgtggatat ggagttatat
      781 cacctccaat ttggccaata aaatctaaat aaactcaaca taacacacct actttttgta
      841 gataatataa taaatttaaa tattgctttt attttaaaat tgaagtattt atttaaattt
      901 aaaatattta ctcaaactaa aaaaatagct atttgactaa tctaaaccat cttaattatg
      961 gtaaatagta aatagctatt tgtaagttat attataaatt ataataaaaa ttgtatttgt
     1021 taaaattaaa ttaaactgct atatcattta atacttttgc ctttacaact cttatatttt
     1081 tcatactctt gtagatatct tgaatcttat cttcttgctc tctcaatagt ctatgtgctt
     1141 catatatatc tacaagttca aattttacta catcatttgg ctttgcttgt gctaacttat
     1201 acaaatctac acttataacg ttccctatct tagtatagcc accactagtc tgtctatctg
     1261 ataacattat aatcggtttt ccatgaccag gtacttgtat agctccataa gaaataccat
     1321 cagatataat atctgctcca ttaaggtgct ttatactctc tccatctagt ctacaaccca
     1381 ttctatctat ttcattcgta actttgtatt tatttgataa aaatttctct acttcaccgt
     1441 tagaaaaagc atcaaattga ggacctttta caattcttag tacgatttct gaactataag
     1501 ttggaatata ttttgaattt aattttttat ttattgtaaa atctttttcc tgaataaaag
     1561 tatttatata atctcctttt ttaagtgctc taccttcaaa tccgccaatt ttagatcttg
     1621 tataagttga cttacttccc attgcaagtg gaacatctat tccaccagct attgacacat
     1681 aggctctagc acctgatttc acacctctaa atgataatac atcacctgga tttatttgat
     1741 atgacctcca gttttgaatt tcttttccat ttattttagc ccctaaatct cccccagtta
     1801 ctgctatttg cgtatattca tcaaaggtaa ttgtaggacc taacattaaa acttctaaag
     1861 aagcctcatc ttcagaattt cctactaata tatttcctac tctatgtgca tactcatcca
     1921 ttgctccaca tccagataca ccatattgac catagccaac tctacctctg tcttgtatta
     1981 atgtttggaa acctcctaat tcaactttga atcccatata taccactccc tgtactataa
     2041 cgatatttaa atacttaagt tatttttttc gatttctata aattcatcta atgttattgg
     2101 aacaaatttt ataaagtctc cagctctaag caaaatctgt tcttctctat tttcatcata
     2161 taattttaaa ggtgtcctac caattatttg ccatccacca ggactatcaa taggatatac
     2221 accagtctgg gaacctgcta ttccaacact cccaccataa attttagtcc ttggcacttc
     2281 taatctaggt gtagctattc tttcatccat accacctaga tatggaaatc caggagtaaa
     2341 ccctagcata tatatcaagt actctctgct tgtgtgtatt tttataactt catctacagt
     2401 gagattattg tagcttgcaa cgtttttaat atctatacca aaaacctcat cataacatac
     2461 tggaatctca tgtattttag gttttggtaa tactatactt tccaaattat cttctatttt
     2521 ttttaaattc tcaatcaaac tatcaaaatc tatttctaat ggattataac ttatcataag
     2581 tgacctatat gttggtacca tttctgttac aataccttta agattcgata tttctattgc
     2641 tctcataaat gaaattactt ttttgtttat atcttctgat atctcatttc caaattcagc
     2701 aaccactgcc ttatcccctg atatcaagta tctagtttcc ataaatgtca cctcttctaa
     2761 attaagcaaa taaagcacct aggctgctaa gagaagttat tcctgtatat gcagatatta
     2821 aaactactat taatccaaat attaaaagcc atgtaggatg cttgtaatct ccaacaatat
     2881 cttttctttt agatgctata agcataattc ctaatgtgat tggaagtatc aatccattta
     2941 aagctcctgc taaaattaat aaagtagctg gttttcctat aaatgccatt ataagtgttg
     3001 atattgctat aaatgctatt ataaaatatt tttcatattt atctataaaa ggatttagtg
     3061 tctttaagaa agaaactgat gtataagcag ctcctataac tgatgtaatt gcagcagacc
     3121 ataatacaag tccaaagaat ttatatccta ttgctcctgc actaaattta aaagctgaag
     3181 cagctggatt ttctgggtca agttgaagac cttttgaaac cactgccagt attgctaaaa
     3241 ataaaagaac tctcatcatt gtgtcaacta atatacccaa aagagaagat ttagttattt
     3301 cttttatatt ttcttctcct gttattccac cgtctattaa tctatgtcct ccagcaaaag
     3361 ttatataacc tccaacactt cctccaagta gagttattat tgggaatatc aatccttttg
     3421 gattttctgg ttttaccatt cttgatactg cctctccaac aggaggatga tttgatattg
     3481 ctacatatcc aactataatt atcattccta atgctaaaaa tttagttatc ttatcgacta
     3541 gagaatttga attttttgac ataaatacaa atatagctat taatccactt aatacagttc
     3601 ctaatgtcat attcatatta aacattacat tcataccaag tgcagctcca ccaacatttc
     3661 ctatattaaa agctaatcct ccaagagcta ctaaaaaagc tactaaataa cctaatcccg
     3721 gtattatttt atttgctata tcctgacctc taagtcctga tacaccaatt actctccata
     3781 catttacttg agcccctata aaaaggattg ttgtaattaa aattacaaat gaaaaatttg
     3841 gtccaaaatc ttgggtaaat tgtgcagtct gagttaaaaa ccctggtcca attgctgatg
     3901 ttgccattat aaatgcagcc cctataagtg ctccgacatc ttttttattt ttagttttat
     3961 ctgtaatatt ttttgtactc atattttatc tccccctcat gttatcagtc ttatactatg
     4021 ttctctgacg aacaaacttc tatgttttct aatggacaaa cttctatacc ttctaattca
     4081 aaacgctttc ttatttcttt gacaaactct attgcttttg gattgtcccc atgtacacat
     4141 atagaatctg ctttgatatg tataacttca cctgttaaag tttctacagt tccttctttt
     4201 accatcctaa caaccctatc tattgcatgt tttgtatcat gtataactgc tccttctaca
     4261 cttcttggaa ccaaaaaacc attattatca taagctctat ctgcaaatac ttcatttgca
     4321 aacctaagtc caatttcttt agcagaattt atcatttcac tattatatag ccccaacagt
     4381 ataatatctt tatcgacttc atatatagct tcacaaatcc ccatagctaa ctctttatct
     4441 ttagctgcca tattgtataa tgctccatgt ggttttacat gttgtatatt acatccattt
     4501 gaacttgcaa atgccattaa agcacccaat tgatattttg tataatcttt agcttcctgc
     4561 tttgttattt tcatctcacg tcttccaaat cctattaaat ctaggaaacc tggatgtgcg
     4621 cctattttca ctccattttt tttagctagc tgtactgttt tttccatatg actagggtca
     4681 cctgcatgaa acccacaagc tatattagct gatgaaatat attttaaaac ttcttcatct
     4741 aaaccaatct tatacgttcc aaaactttct cctaaatcac tatttaaatc tactttatac
     4801 aatttatgac cccctccttg ctaacatttt gatagaataa gtagttttaa tttttaaatt
     4861 ttgtttattt tatatattgc atgaaatatt ttatcacttg tattcaattt tatttttgca
     4921 actattctat tgcatttaat taatataatt ttaccattta aggaatattt ttgcaattat
     4981 tttaattgaa cattcatttg tatttctttt tcaaaaaatc tttttaccat tttttagtta
     5041 ataattaatt attttcactt aaaataaggg ccttaacctt catttttaaa gtatttttca
     5101 tttttaatca ctttatatgc aagacagaat catttttctt catttatttg tttctttcat
     5161 ctatataatt atcattcttt gtaagtaaaa tatctttata tttttataat taattcttta
     5221 tatagtttta agtataataa aatcatgtat actaaatgca tgttaaaata ttattttaaa
     5281 acatgcacaa tatttgtaca tattgtagac atataaacct ataaattata caatagtatt
     5341 gttaagatta tatatttttt taacataaat tttgtattgg gggaaaaaca atggataaaa
     5401 actatacgat gccaatatac caaaaaatag cgttggatat tgcaaataaa atatacacgg
     5461 gtgaaataca agaggattca gtactatttg gacgctctgt tttggctggt aaatacaatg
     5521 tatctccaga aacgataaga agagctgtca aaattttaga ggacattggc gttgttaaaa
     5581 gcataaaggg taaaggtgtt attgtactat ctccagacaa agcttcttct tttataaaaa
     5641 aatatagaga tattacaaat atatcatcat ataaatcaac actttataat ttaatagata
     5701 ctaaatctaa tttagaaaat gaaatattag atactataaa taaaatttta gactattcca
     5761 acagacttga aataatcaat cctttagtgc ccgttcagtt caccataaat tctaattgca
     5821 aatatattgg tcaaacagct gcacaaacta aattttggca aaatacaggt gctacaatag
     5881 ttgcaattaa aagaggtgaa gaacttataa tatctcctgg accatatatt gaatttttag
     5941 aaggagacat tctcttagta gttggtgacc aacacattta caattctata ccaatgtttt
     6001 tatatgaaaa cgaaaaataa attatataat ttagtatgat tatataaaaa tgcaaaatta
     6061 acaacaaaaa aagagcttca tattttacat aaagctcttt ttaaattcaa ataaatttaa
     6121 ctttgattaa tttttaaata tccaattcat cttctttttc ccagcttaaa gctctcgata
     6181 cagctttttt ccatccttta tataatttac atctcttctc ttcactcata tttggattaa
     6241 actctttttc tactgaccac tttgaagtaa tttcatttct tccaatatag aatccaacag
     6301 acaaccctgc taaataagca gctcctaaag ctgtagtttc tactattttg ggtctatcta
     6361 tatctacatt taatatatct gattgaaatt gcattaaaaa attattatta cttgctccac
     6421 catcaacttt taatgatttt agttttaatt ttgaatcgtt ttgcattgct tctaaaacat
     6481 cctttgtctg ataagcaatt gattccaatg tggctcttac tatatgttct ttttttgctc
     6541 ctcttgtaag tcctaaaatt gaacccctag catacatatc ccaatatgga gctccaagac
     6601 ctgtaaatgc tggcactaca tatacaccat tagtatcctc taccctattt gcatacattt
     6661 cactatcttt tgcagattca attatcttta attcatctct aagccattga acagatgctc
     6721 ctccaataaa tatacttcct tctaatgcat atgttacttt tccatcaact ccccaagcaa
     6781 ttgttgtaag taacccatgc ttagatttaa ctatgttact gcctgtattc ataagtaaga
     6841 aacaacccgt tccataagta ttttttgcag taccttcttc aacacaattt tgaccaaata
     6901 atgctgcttg ttggtctcca gcacatcctg caataggtat tttagctcct gataacatgc
     6961 gctcatctgt atgaccataa acatagctag aaggttttac taatggcaac atgcttattg
     7021 gtatgtccaa ttctcgtaat atatcctcat cccattctaa atcatttata ttgtacatca
     7081 ttgttctaga tgcatttgag taatctgtta catgtacttt cccacgagtt aaattccata
     7141 ttaaccatgt atctatagtt ccaaataata actctccttg ttctgctttt tccctagctc
     7201 cttctacatt atctaatatc cattttactt ttgttgctga aaagtatgca tctattaaaa
     7261 gacctgtttt ttcttttatt gattcttcca atcctttatt tttaagttca tcacatattt
     7321 ctgatgttct cctacactgc catactattg cgttataaat aggtttacca gtatacctac
     7381 tccaaacaac cgtagtctct ctttgattag ttatccctat tgaagcaatt tcttctggtc
     7441 ttatacctgc tgtttctata acttctctca ttacaccact ttgtgacccc catatttcca
     7501 tagggtcatg ctctacccat ccagcttttg gataaatttg attgaactct ttttgtgcaa
     7561 ttttcactat ttcgcctttt ttattaaata gtatagctct tgaactggtt gttccttggt
     7621 ctaatgccat tacatacttc ttttccatca ccatattctc cttatcatat aatatttaat
     7681 ttattaaaaa gaaaaattta atatcacata tttcaataaa taatcatact taagtacaaa
     7741 tttaatataa tagatattta taaaaagaca cttattttct atcatatcta tataataata
     7801 ttatacatta tagcttacat attgattaat aaattttaaa ggagtataat aaacagtgtt
     7861 cataataatt ttaataaaag aggtggtaat atgccaaaga ttttagaaaa tgtaaaagaa
     7921 gatatattaa aagtatctcg ggatatgatt ttagaagaag attactcgaa tattagcata
     7981 aggaaaatag ctcaaaggtg tggtataagt gcaggtacag tatataatta ctttaattca
     8041 aaacaagaaa taatagaata tatcacaaaa tctgaatggg atttattgat aaggcgaatt
     8101 gaatactcca ataaaaacac tgaagattac ataaaaaaac taaacattat ttttactgaa
     8161 ataagaaatt ttataaacaa agttcataat atccaataca atgacttttt aaacactttt
     8221 gaaacagaga gattttttga aatgaaaaaa cacaaagacg actttcatga acagctttca
     8281 aataaagtat atgaagcttt agaaaaagaa agttttttta ataatgacaa attagtatgc
     8341 aatatcataa taaaaatgtt tttttcatat tcaacatatc ttcatataga atttgaagat
     8401 ttaaaaccct atattgaaaa attaatacat taaggtggtg tttaccttga atcataatac
     8461 tagtattaag aacaaggaca gtaatccaaa aatattttta gtacttacac tttatctatt
     8521 aggtatattt atgggagcta ttgatactgg aatagtatca ccagcaagaa cgattataca
     8581 aaatagcctt ggagtaaatg aaaaaacggg aatttggatg attaccatat acacattaac
     8641 atacgcttct gtaattccta tctctggaaa attagcagat aaaataggaa gaaaatatgt
     8701 gtatctagtt agtatatttc tatttggtag tggttcatta atatgtggat tatcatcact
     8761 attttccaat ttctatattt tattaattgg tcgtgttatt caagcaattg gtggtggtgg
     8821 aatcatgcca attgctacag cagaatttgg aactaccttc ccagaaaata aaagaggcat
     8881 ggctttaggt ttagttgggg caacatatgg tattgccaat attttaggtt caagcattgg
     8941 tagcacaatt ctaagtatct ttggaactca aaattggaag tggctatttt ttgtaaacct
     9001 tccaatttgt ttaatcatac ttattggtgg agttttctgt attaaaaaca ataagtctga
     9061 atctaaagaa aaaatagata agcttggtac attgatgttg atatgtataa tcgtatcact
     9121 attatatggt cttatgaata tagacttttt taattttaaa aattctatac aagatatatc
     9181 agtttatcca tacttattga catttattgt acttatacct attttcatat ttattgaaaa
     9241 tagagctaaa gaccctattt taagctttga atatttttta aatccgagaa tattgatagt
     9301 acttatttta tctttaattg ttggtattgg aatgatgggt atggtattcg ttccacagta
     9361 tgctgaaaat gcattaaaga taaatgctgg ttctggtgga tattttgtag ctatattggg
     9421 attatttgca ggtattgctg ctcctttaag cggaagactt attgacaagt ttggggcaaa
     9481 gaaaatacta ttactaggat tttctgtatc tatgataggc tctctttacc tagttttaat
     9541 tgctctaaaa accaatactg tatttagtgt atgtgtaagt ttaatattta tgggattagg
     9601 aatgggcttt actatgggaa ctcctctaaa ctatatgatg ttatcaaata caagattaga
     9661 agaatctaat tcagcacttg caacattatc cttgatacgc tcaattggta cttcaatttc
     9721 tccagctata atgattggat ttatagcaag cacatgcagg attatctgtg caagataata
     9781 ttatggactt agttggaaag ccctcaactc ctaaaatagt tcaacttgaa gaacttgaaa
     9841 acatgataga tgacttaaaa tcagaccctg aaatggcaaa aaatttaaaa aatgtctcta
     9901 tcccaaatat gaatgatact tcaaatataa atatggacat gacaggtggc aaacttccaa
     9961 aatccttact taataaagtt cagtcagcag atacaaccaa cattaccaat ataacaaaag
    10021 aaatatcaac aacaatgttt gatgaaaaag ttccatctat aattactaaa atacagaaaa
    10081 atgtacaaaa aggaatagat ggaacacaaa gtggaataaa cggtattgaa gatggtgaaa
    10141 ataaactaaa tagtggtatt aaaggagttc aaactggtat tgacaatatg gtaaaagcta
    10201 gagctggtat taatcaagga atagatggtg taaaaaaagg tattgctggt gttgacaaag
    10261 gtataaaagg tatggaacaa ggtcttgaaa aacaagatga ggccatcaag gaacttacaa
    10321 ctgcttataa taaaatacct actacagaaa agactcctac tgataacgaa aataactcaa
    10381 atgaacaaaa ccagagtaat accagtaatt ctgaatcaga cgacactaag aatgattcta
    10441 cagaaaataa gaataacaat tccagcgaga ataaaccaaa tgtaaatgaa caaaaagaaa
    10501 gtttaaatgc tcaaatacag caactaaaaa agacaagaga tgacttaaat acaaagttac
    10561 aaaaaaataa aactcaaaag aacgaattaa gtaaaaaact aagatcaatg gaatctcaaa
    10621 agaaagagct acaaagcaag ttaaataatt ctataaacca aaagaaagac atggaaaaaa
    10681 gccttgatac aatgcaacag caaaaaaaat cactccaagc agttttagca aagacacaag
    10741 aggtaaaaac tgaaatacca aaggcttttg ataaatcaaa attggattat ataaattcca
    10801 tagaaaataa tagaacaaaa atagaaaata cactacaatc tactctaaac tcaggattca
    10861 aacagatgta tataacagta ttctgtgtaa atctactagc atttataata ttattgttct
    10921 ataaagaaaa taaaactaga taaaagagtg ttatagtatt atattgtaaa tttatcttat
    10981 tcttaaatat tttaccctaa agaataaatt cataaaatct ttgtaattga ttaatataaa
    11041 ttacaatata atactatttt tatcttaatc tttttctctt ttacagacaa tcaaaaagca
    11101 atcttagttt atcgaaataa atttatacaa tctattcata caccccttta catttttata
    11161 acaattaaat ctattgttat atatacaaat caaaataaac catatatttt caactataat
    11221 atgttaaaat catattatga gcatattaat ttaagcaaaa tgtcatttag actttaattt
    11281 agtagtttta tattataaat ttatatattt ttaatttcaa atgtcattta gacattactc
    11341 ataaaaagaa aggagtgtat tttgtggata ctttaggaaa aagaattgct tatttgagaa
    11401 actccaaaaa acttactcag cgcaaactta tggatatttt aaaatttgaa aacttaggca
    11461 agtatgaaac aggagataga aaacctaatt gtgatatact aatgtcaata gctgattatt
    11521 ttaatgttac gactgactgg cttctctatg gtaaagagaa ggtaaatgtc aatagtagtg
    11581 taaaagaaga taaagaagat tacttacatg ttactaatga tgaaatgatg atattaaatc
    11641 tatacagaca actaaacgaa cgtgataaaa taaaaataga aggaatactt gaacttaaga
    11701 tatctgaata taaagactta aaaaagcatt cctctaataa taatgaagat aaaatggttt
    11761 aaacatacat aatttgatac gattttacat catctattaa ccttcttttt ttgtagtata
    11821 ttactaaaat atggatattg gattatttaa aaacaagtat agtctaattt caagcacatt
    11881 tataatattt agattatctt atccatattt tagtttaagt actttgaaaa atataataca
    11941 tttctatgta taatttatat tcctaagaaa tgcattactc atgtttttaa ttcttccaac
    12001 atctttctat acacaattgt acatccttca taattcccct ctttaagaag gattgaacat
    12061 gtactagaat atttagttaa agtatttaat ctttccatat ttaagctttt tatattacta
    12121 agtgctgtaa taaaatcatc aattgctcta caacactcta taaccccatc tgaatccaat
    12181 ttgcacacaa gaatattcac ctactttata tactatttat taatcatctt ctgaatattc
    12241 tatattaagt aattgataaa ttgatgattt atatttttta cctgttcttc ttccatttaa
    12301 aatagctgac aaatagtttt cattaacacc tattttcttc gctaattccc tttgagtcat
    12361 atttaattca ataagtcttt gcttgacttg cactcccaaa aaaggtatcc atttttttcg
    12421 agcatccatt tatcaacttc ctttctaagt gatttatatt tacttttata ttactttagc
    12481 ttacgcaccc cctctattta gtatatattt ttaatatgta cacatattaa gtatataaat
    12541 ttatttaata ctaaataaga ttttttgatt actttgtgct aaaattagga tgtaaattcg
    12601 tatcattaag ttaattataa agtcattttg accttaagtc aatggttttt atgtcatttt
    12661 gacctttaat ttgaaactat ttacatctta aaaagtcttt ttgacattaa ttgaatggag
    12721 agtgtttatg gatactttag gagaaagaat tgtttatttg agaaaagcaa aaaaccttaa
    12781 acaatatgaa ctagaagaaa tgttaggctg tgacaactta agtaaatttg agagaaacat
    12841 tagaaaacca aattatgaaa tattaaaatc tatagctgag atatttaatg tttcagtaga
    12901 ctggctatta aatggagata atctatcaca taaatcagat ttaatttgcg attcctcctc
    12961 aaactatcca cttaatagca taaactctaa tgaaataaag cttttaaaca actttagaaa
    13021 gttaaatgat tatgataaag ccaaaataga aggtatgatt gaattaaaac ttcatgaata
    13081 tgaaaaagag aaggatttag gaaaaattga gtataacaaa aataaagatg agaaaataga
    13141 taaataattt ttatgttgct tttgatacga ttttacatca tttctttgtt ctaatatttt
    13201 tatttcattt attttctttt ttataatttt atgataaaat aattagtata ttttgcttta
    13261 cttgaatgta attatttata caaaagtcaa acataatata aatgtagtaa atttttagtt
    13321 tcaagtaata tctcgttatt ataactaaat atttattaaa attaggagga taaaatgggc
    13381 ttaagagaca aattcgcgca atcatttgct agatcaaaaa ctatgagtgg ccctgagaaa
    13441 aaggctaatg aaataatggg taaattgctt cttaagaaag ctatattacc tatcgtttta
    13501 atgttcgtga taataatcgc tggagctatg cttaaaatca attcttgggt tacacttggc
    13561 ataaatttag ttattgctgt tggtgctttc ttctacataa gaaattctag taaaaagtat
    13621 cagaacttta aaccttatgt tggcaatttg attagtttag agaaaaaagg taaaaaagag
    13681 tacgttgcta ttataaaaca aggtaagtta ccagttaagt tacaaatagc ttatggtggt
    13741 gaagaccttg aacatgttaa gaaaaatcaa atggttcaga taagttataa tccagatgct
    13801 aaaatagcta ttttggttaa tagacaataa tttaataaaa aaaaggagtt gtcttaaaat
    13861 tgattttttc taatcaaatt taagcttaac tcctttttga atttttatat gttattttta
    13921 tatattattt ttattacttt ttttaattaa tattttaaaa tataaaatcc ttctcaataa
    13981 aatagggcac tatctacaat actactttat cttttcctaa taatatataa tctggtgcta
    14041 ctgctacttt catgttatac accgttttgt aacatacctt ttatatcagt aacatcttcc
    14101 ttaatttcct ctacatctgt tttcattatt cccatttgaa caagtatatt tttgtttatt
    14161 tcttgttgtt gtgtagataa ttctataaaa ttttctacag tttttttata catgtctcta
    14221 tcttcttttt tctcctgcat agtattttta aataatagag cacatagtat acctattgcc
    14281 cccagactac tcaattcggt tattaattta tccattatag cctcctattg tctaaatttt
    14341 ttatacttat agctttaatt tttgttatat tcttacatca tgcaatatta tattttcagg
    14401 attatcaatt taatcttctg tactttaaat tgtaggttta atttatagat tgaataaata
    14461 ataaatatat atacaatttc taatataaca aaaaataagt acaaggctta cttattaaag
    14521 ttaagacctt gtactttaca taaaaaaaat aactaataca tagttattct aaatttacta
    14581 ttcaactggt atttctaatt ctttacatct aattctaact tgctctctaa ttactgctgg
    14641 tacttcttct attgtttttc tgcctcttaa aattaaagct acatatactt ctaccattcc
    14701 actctcacct ccagattttc ttacactaaa agtgcttata ttacttgctt cattgacaga
    14761 actttgactt aacaacatct catatatttc tgcatttgct ataaaattat ttgttatatc
    14821 atcatcttgc tttgcttgac ctatacttat gtctgaaatg tctttatact gcatttctac
    14881 ttttctacct tgtttgattt gttctatttt atttttatcg aatttaaaca atttttacac
    14941 ctccatttta agcttgtggc tcgttgtttt cagcatcata tctaaaaatc atagatggac
    15001 gttttatagc tgtccattta tctccttttt ctactatgat tttaggctga gcacttttta
    15061 tgttggcttc ttctaattca tagatttctt gataacttga gtctgatttt ttgatttcaa
    15121 ctagtttcat gtctttgaat aagtcagatt tatttatttt tacttgaact ttagttttat
    15181 ttattaattg atatataggg tctccaagat ttaaatcttc ttcaagtcct ctatctagtg
    15241 taattgtaat atcttgttca ttagatgttg tattaactac cttgtacatt ctattaccta
    15301 tcataacata agaatcttta attatagaat ttccagtact ctctttaaaa ataaataaag
    15361 atgcatataa atcatcacct ttacaataag ctctaaatag tatcttattc tctcctattt
    15421 taattttatc tggtggtact tcaaaggata ttttttcagt agttaaatca cttacagatt
    15481 ttacactttc accatttata agcatttcta ttctacttat atctttatta gggtcttgta
    15541 tttttatctt aattgatgaa attgattttt cagtaatttc tatagcagca tttgtcgttc
    15601 ctacttctga tataatatat aatgtattgt ctgttgcaag tacatccttt actgtattta
    15661 catcttcaga aataggtact atagtaaatt cttttaaaaa ttctactggt tgttcaattt
    15721 tatatacatc cctatttttt ccagtaaccc atactgaatt atcattttta agtactaata
    15781 tatggtcact tccataagat tttatttctc ttatgtctgt tagtgctttt gtaaagaact
    15841 taattatagt attttcacta tcgcttccta atcctaaagc tcccttatca ttatatcctt
    15901 gtacaaataa ttcattacct gtagttacta ctaatgtata gtttttacca caatgtactt
    15961 ctttacagct agatatagtt gtaattcttt gtaattcagt aactggatta ttgtgaccta
    16021 tacctaattg atattcggaa tttttaccaa tagaccaaag actatcatca gattttataa
    16081 aataagtaaa gttatctcca catgaaacat gctttacatc tgatacatta atctgcacaa
    16141 attcatccac ttgatttata gacaagtctt gcaataattc tccatggtag tttgtaccac
    16201 atccatacat tgttccatca ttaagtaata taataatatg agtcttagaa atagatactt
    16261 cttttatatc ttcatatgga agctcagtat aaaggtctcc tttgtataaa tttcctaata
    16321 ctaaaccata atctatgtct tttgtggtag gtatcactat cttattgttt agaagtataa
    16381 agttatgtct gtttttaata gatgtcaaat cacccattac aaaattcata tgcttataat
    16441 taaatattct attatcatta tttctactat cgtatctatt tccaacacct aattgtccat
    16501 agttatttaa tccacaaata tatatatcat tagtatcttt tattgctata tttgcatatt
    16561 ctaaaggaag tattttcttt actcttgtat tggtttttat aaatgtatct ctttttgtta
    16621 catcaccaac acctaattgt ccattattat taaaacctgt accatatagg tctccattat
    16681 cacttacata atgtccacat ctataattca ttccaatatc ctttgccttt gatgctattt
    16741 ttttaatctt ctctggagtc tcacttcctg tacctgaacc attactacat ttgcctgcag
    16801 tatatacatc cccttcactt gtaagtaaaa gtactgcatt atttctgttt ggagctactt
    16861 ttactacatt tgaaattgga atttcagaaa atgaactttg attgctatta ctatttagac
    16921 ctaagcaata atcagtattt aaacctgtac tatatgcagt attatagatt gttattatat
    16981 aggtatttat ttcatttggt ataacttctt ttacattttc catacacttt ataaaactat
    17041 actggtcagt agtatttccc atacctagtt gtgaattatt attgtatcca caacaatatg
    17101 caatgtttgt gtcctttaca ataaatgttg ttgtatctga gcagtacaca tctcttacat
    17161 tagttatatt gtttacagta aatgtcaata cgtctggatg gttgctttcg cttcctaagc
    17221 ccagttgccc atgagtgttt tctccacatg aatataatgt gttatcatct ttgattgcat
    17281 gcgagtgagt agtccctaaa acaacatctc ttacattttg gatgtttact ttggtaaatg
    17341 tatttctatt tactatatca cctaatccca gttgtccttt ggtatttaat cctgtggagt
    17401 atagtgttcc atcatttcta attgcaaatg ttgtgttacc atctatactt gttgatattt
    17461 tctttatatt atcaaggttt atctgagtga acttatttct atttattgtg tcacctaatc
    17521 ctaattgtcc agaactatta tttcctgtgg cataggctgt tccatcattt tttactataa
    17581 acattgtatt tcttgatgtt gtaatttctt ttatatcatc cataaatata aatgagtttg
    17641 attgttcagt tatatttgtg aattttttta ctggttcgtt gttatattcc cctatcccaa
    17701 gtttgttatt ctgattcatt cctgttacat atatattgtt atttttatct actactacta
    17761 cagtatttcc ttttacaatt atatctttta tgtcttttaa tcctgtatca tagttacttg
    17821 tgccatctat atttgttgaa ttcccaaatt gcccactatt agcaatccct tttccatata
    17881 acttggaatt actatgatat attataaaat tacttcctaa attagaactc tcagaattaa
    17941 tactagtata acttctatgt ccagtaatgg ttgagccaaa tgtttttgga tatccagtaa
    18001 tatatgaact tcctttctta aaaaataata tattatttga catccctatt actttttcaa
    18061 ttccactaaa atcagtctct tgtataaaat aagtattatg atttgaattt gtagcctcaa
    18121 taaaagttcc atatcttgaa taatcagata tacttggact gctatttaat gttcttaaat
    18181 atccatgata tcctgttaaa taaacataac ctccttcgtc aattattgca caaccatcat
    18241 aaaaaggaaa aacattagtt attttaggta acgttctaag tccatttgat gtagaagatg
    18301 ttgaatttgt tctacattga gatactaatg ctttgctttg gttactatta tttcctttac
    18361 ctaattgata attataattg tatccagaaa catatagttt cccttcactg tttatcatat
    18421 atacagaata atcatatgct gctatctttt caatattact tattccctgc ttatatggat
    18481 aattagaagt tttttctata ttattataag gattatttcc ccatccatat acttcttttg
    18541 agtgatttaa agctatgaca taattgcttc ctacacaaaa atcctttaca ttctctattt
    18601 ctatttttgt aaatatattt ctattaactt catctcttaa acccaattgt ccattactat
    18661 ttaaaccaca tgaatataat gttccattat tcttaagcac aaaagttgag tttccgtttc
    18721 cttttatatc caaaacatta tctatatcca ccttagtcat aactgacctg ttaacagtat
    18781 ctcccaatcc taactgtcca taattattta aaccacaagc atacaatgta ccatcattca
    18841 taacaataaa catattgcta tctgttaact ctatcttctt ataatcttga aaattatcac
    18901 ttaactttac atttaaaaat tcactattgt cctttgtaac atataaataa tcatcattaa
    18961 ccaataaacc tgaataatta tctaaatttg aagattgcac atttgaaaac tcatacagaa
    19021 aagttgtatg aaaaaatgaa ccaagttgac ctgctaaatt acagccagat acaaatatct
    19081 cttttgaacc atttattaaa aaattaaatt gagaaccaca tttaatatct tttatatttt
    19141 ctacattaac cttagcaaaa gtatttcttg atgctacatc tttattggca ttggctagtt
    19201 gaccataatt gttttgtcct gtactaaaca gtgtattatc gtattttaat atcatagtat
    19261 gattatttcc acaagaagca tacttaacat tatttatact agaaagagta aatacaattg
    19321 aattattatt atttcctgta ccaagctgac caactccatt atagcctgta gtatacatag
    19381 ttccatcatt cttaataaga attgtatgac tactaccaca cgctatctgt tttacattat
    19441 ccataccttc tattttatta aatacacttt tatttaaagt actattaatt cccaactgcc
    19501 catttgaatt taatccacat gcatgtgctg tcccatccat ttttatgata aatacagatt
    19561 gattataagt cactacatct ttcacactat ctatatttac tttagtaaaa gtagctctat
    19621 tgttagtatc acctagtcca agttgaccac ttgagtttaa acctgctgaa tataatgttc
    19681 catcattttt aagcacaaat gtaaagtcat aaccacatgc aacagttttt acatcatcta
    19741 ttggtagctt agtaaattca taatatactg tagactcaat tacacctaat tgacctacat
    19801 tacttgttcc tgctccatat gcaaatccat ctttagttac tgctacactg tgttttgaac
    19861 cacaagatac atattttaca ttatcaattt ttactttact aaacatcctt ctctctgacg
    19921 aatcttttcc aagtcctagt tgaaaacctg tattatcgcc acaagcatat aaattaccat
    19981 ctgattttaa accaaaggta ctattactta taccagtaaa aaattctaca aaatcataat
    20041 cattatatct tttatcaaaa tcatcaatca atatgttctt atcattaaaa taagcaccac
    20101 gctgtaaaag tttattttgc ttcattatta gtactacctc ctttatttat cttatttcat
    20161 atgaatattt acttagagtt atatcttttt gttcttttaa ataagtgtta ctagattttg
    20221 tctcaacaaa gttattatcc atatcatgta tatatctaac tctatttgca ctaaatggta
    20281 atactacatc tctaaaaatc acattaccaa tttcacctgt tatacatttt ctatttaaat
    20341 ctaacttaag cccttctata gctactatgt tatttccatt tgctaatgta tcataccaat
    20401 aaccagcctc atctacagtt acttctgact ctaattctag gtatattaat atatctagca
    20461 ctttttcaga taatttgtta tagctatttt ttaatactga tatatcagtt tgtatttgac
    20521 cacttataac atcatcaaaa gatatatttt ctattttttt aactacctca tttatagctc
    20581 ctgataaatc tttagaatct gtagttaata tatctgtatc tccaattttt acttttattt
    20641 cttttatatt tttatcaaac ttatcatcta aatcttgaat atccttttga gttacaaaaa
    20701 ccaacgtcgg gtctacttct aatgtaacat ttgaagtatt ggacaattgt aaaatcattt
    20761 ttacaaccaa atctttaata ctgccatctt cagcacgtgg cttatacgtc tctggatact
    20821 tacctatagc tattatatta ttctcattat ccagaacagc agcttctctt atcataaatc
    20881 caccaactgt tcctggtaaa atagtctcta taactatcca attaggattt ttttcatcaa
    20941 ttcttagaga atttatattt ccttcccaaa ctacattctt taaagcagtt tgttcctctg
    21001 ttggctcata atattctcca ttactatctc caacttgaaa cttggttaat ccaacaagct
    21061 ctcctagtgc agttgcattt gctatttttt ctctaccaat tttggttaat attatataaa
    21121 atttttcttc caattatacc acctccgatt taggataaac tactacattt tctacgcctg
    21181 tatcatttgc aatagctata ttaatcttac aattattttc aatgcattta atgcttaaag
    21241 gataaacctt tatttcttct ctacttaaca ttccagctcc acagtttaca acagcactac
    21301 attttataat tggttcaaag tatggatgaa cttttacctc ttcactacat accatacctc
    21361 caccacaata gaccatactt ttatctagta ttattggttc taaatagtgt aataagtgag
    21421 caggttttac tctttcaatc atctcgctac aatctaaaag agttttatag tcacagttat
    21481 ttgctataaa actcaatacg aatgtaaatt catcactata aacttttata tctgtttctg
    21541 attttgtata tgcctcacat atacttttta taacttcaat agtactagta cctctgcttc
    21601 tcattttagc ttttatattg ctacgtcttg tgtcaaaatc aagttctttt ttttcaatgc
    21661 agagtatgtc ttcccacata tctaatcccc atgtcgctgt atcaacaaat aattggttaa
    21721 aggtatcatc aatatcttct ttaagaatat ttaattctgt atcgtatgca ccttgtatct
    21781 cctctacaat gtaatttcta tcaaatgatg gtagtttatc aattagcttc attgattttc
    21841 cacctcacta aaattaacag ttgttacact aggtatttta tcttcattaa tagttatatt
    21901 atctgtactt ccatttataa gtagattact taaatcgtgt acaccagtag tatttataag
    21961 tattcccatg acttttgtat agattatctc tcctctaata tctctaaagt atgtatttat
    22021 actttctagg aaagattctt ttacattgtc taatgtatat ccatcttcta gtttcattac
    22081 tgcacttata cttatttcta ttggtaatgg tgtcacaact gttatagttg gtccaatagg
    22141 cttctcttca tctatatgtt gctgacacct ttcaatcgtt tctgtatcaa cagcttgatt
    22201 attttcccca aatatcaaga ccttaactgt tcctggacca tcccatcttg gataaacctt
    22261 aacattatag actccatcta cttccaaagc ccattcttca tagtgagctt tatttccact
    22321 tgtagcttga tttctttgga ttttataaaa tctttctttt agttcttcat ctgtctctat
    22381 atctgtacca ccttcaaaac ttcttgtgtt agtaatcttt gttactccag atatattatc
    22441 aactagttta aattcacaat ttgtaggtaa attatatttc ttaccaactt ccagagcttg
    22501 aactgggcta ttgtcacctt cttcactacc aatagttaca tcttttatta ctacaaatag
    22561 taaatctcta tatgatatta ttgtgccatt atttattaca gttcctttct ctccaataaa
    22621 ttcaacttct ccatttgact ctgtacctaa ctttctatat acaccaaatt cattgactct
    22681 tttatcaaga aactggttat atgtgtcttg tataaaagcc attttatgta tctttgaaag
    22741 ttctagatat atcttcgaaa gttctagatt atttccagat accatgttgt ttagaaaaga
    22801 accttctcct ttataaatat caagattaat attttcaaga gttctatttt ttattacttc
    22861 atatgtctgg tcactataca tttatttcca cctccccata gatagtggat acttttacat
    22921 ttgcactcaa tacgtctctg ttaaagttag cactttttac gtttacttct aatatatatg
    22981 gatttattag aagggcctct tttatgaatc tactagcttc actttctgta agaccttttg
    23041 tatatttttg tcctattagt tctgaaagct ctgtcccata ttctaaagag tatatctcat
    23101 gttcatatct atttgtcttt atacacttgt atatccaaac ttttaaggct tctttttttt
    23161 caattatttt aaagtcacct ttttctaaaa taggttcatc tttttcaaaa tcccatgcca
    23221 cttcacgaaa gattggcaat tcttctgttt taggtaagat ataatcctct gggacaccta
    23281 taaaaggaaa tattgtactc attataagct caccaactta cttacaacag caaatttttc
    23341 gcctattcta aacattatta ccatatcccc agattcaaag ttatctataa aaggattttt
    23401 tacttcatgc tggtgttctt gatttgtttc tgtgtcaaat gtttctagct gtctatcaat
    23461 catccaacta tctatcaaaa tatcttcttt ttctaatatg atgttatttg tctctatttt
    23521 taaatctggt aatttacttt taatttttcc aataaaaaaa gaaggttgat tatgatactt
    23581 tccttcttct cttattattc ctataaattc atttattgga ttagccatta tatcaccatc
    23641 cttttataat actctcctag ctgtattaaa atctcttcta ctacttaact tacttatttt
    23701 aactacatca cctttttgtg gagcatgtaa aaattctcca ttacctatat ataatccaac
    23761 atggctgact ggattatgaa aaaatactaa atctcctgct tgaagatttt tttgttctac
    23821 tttcttgcct tttttagatt gttgatttga cgttcttggc aaactaacat taactttttt
    23881 aaaacagtag accattaaac cagaacagtc aaaactactt ggtccatttc caccccattt
    23941 ataaggttta cctagatgtt tttttgcctc tgatattact attttagctt tatcagatac
    24001 attaccagaa taaccaccaa taataatttt tccttttctt cttccaaatt tactagcttc
    24061 ttcaacattt ccaaataaaa tatctatatg gtatgttcca tttttttcta tagttattgc
    24121 aggtcctacg tcatttactt tatagactcc atcacgacca gaaactcctg taattacctg
    24181 tatttcatct ccatagttca ataaaggatg tttatttaaa aactcttttg tataataagt
    24241 ttgctcatat ttaccaacca taggagcagc gcaagttttt ttagatgggt caagtttttt
    24301 ctttctacaa tctgtatctc caccttcttc ttttctagga cagtaagctg taaattctgc
    24361 tgtaaactct tttcctcctg cataatcttc tccccctaaa ttactttctt ccttaggttc
    24421 atcctgtcct gctgactttt catccataag attttgaaaa ttaagttcaa gctcaatttg
    24481 atattctcca ttttgccaag tatgtttgtc tgtatctata taaaatagtc ctacaagctt
    24541 tgtataagaa tctttaactt ttactcctct accagttata caacttacat ctccataacc
    24601 tttaagagaa cagctttttt ctatcccatt aaactcgcta tcaatatcta catcttgatt
    24661 ttcttgttgc tgaattactt tttgcattat tacatttact tccttaaaaa tttcattatc
    24721 tattttttcg ctaatcttgc ttccatactg gtctaccact attactttat tttttacatt
    24781 ttccatgctc tccgaaaagg tggtatttat aatattaaat ccctcttcaa acataacact
    24841 taatgtaaca gttccttttt caataacatt aaacttatct aaattagcct ctatcatata
    24901 ctttttcttt gtctttttac ttgcctctgt atatgcactc attattgtgt cataaccatt
    24961 tactcctata aacatctttg tatacttgac attggtcttt gctattgttc caactgaaag
    25021 cctattttca gcaaatactt gctttgctat gtcttcaact aacttatctt taaaattgta
    25081 tgacacttca ctttgtgtaa gtaaaaatcc catatcttta gatacaaaac taatttcatt
    25141 actgcttgaa tctttagacc tattaattat cattcctcta aagagttctt tctcatctac
    25201 atagaaacag actgtactag ctattggtat atcgatttgt tggaaattta catcaaatga
    25261 tgattgtatt attgaaaact ctagtgtcct tgatggagat ttataatcac ctgaccatga
    25321 taccttgtct actatgtcag ttatgtcata tatacttccg ttttttatgt gtacccaaat
    25381 cttaataata aaaaatcacc tgccttttca tttaattcta tcagcagtta cttacaactt
    25441 attatctata atagttattg ttttggagtt acttttggta tctttattct tttatactct
    25501 tttaaatcta atgtaaaata tacatctcga gtcccatctc tttcttcata actaaaccct
    25561 tcaattatga cttccatatt tatatttgtt tccgtaattg taaatcttaa tataaaacct
    25621 tccttcatcc atttttctat cttatttaca caatcatatg gtgatggaaa acctgtatag
    25681 tcacagaaag tgtagtcttt atttggaaaa aaacttgata ttgaaatgct tttaagagct
    25741 acaccaccaa aagttgctac ttcacctatt ttgagtatac tagaagagtt tacagcagct
    25801 tttccattaa tactaaagga agatggaaat actggaaatc taaatctatc ttctgcttgt
    25861 ctaagccaca tttccattat aagacacccc ctaactgtgc caattggata cttgcaacta
    25921 attttgatgt tatcttttct atatcagctt cttctctaat cactattgta tcagctaact
    25981 tagctatgtt tatatttcca ccattttttc tgcttctata ttgatttgct tcttgtttag
    26041 ttaaaaccat ttcaccttca tggagtctgg tttgatagtc attataagga acatagttca
    26101 atccaaatgc attattacct ttcttacttg aataagcttt accagaatca ccagcctttg
    26161 ttccagcata tggattatct ttgtaatttt ttttactctt ttccactaga tttaaaactg
    26221 ttcctgtgat gcttccattt tttatcttat taatcatagt ttgccaccat ttgcatatat
    26281 ctttaactat tttacatact ttatctaata tgttaaaaaa agctcccaat attggctcta
    26341 taattttcca tccagtttcc aacagaggtc ctaaggtctc ccatacagtt ttccaaataa
    26401 cacccagtgt ctctataaaa ttgtttattt gaggtgcata ttcacctaaa aaagtaaaaa
    26461 ttcttgtgat aataggacta acagcttcaa ctacaggtcc gattagttgg aatgctgtag
    26521 ttactattgt accaaatata cttattaatc cttgtgcaat tggtgattta ttgtttatac
    26581 tatctagtaa attatttact aagtttacta ctggaagtag tgaatctatt aatgctgtaa
    26641 aatcaatatt ttgtaatagt gtacttcctg cgttaacaat tgcttttggt gcattcccta
    26701 tcattgttgc tgcatgattt tcaaaacgtt cttgcttatt ttcaaccatc attttacgct
    26761 tttcagcctc attagaaatt cgatattgag tagaatcaga aaactctttt ccatttttta
    26821 cacttctaga ttgcatctgg tcatatttgt tgccaataat tatcttacca ttcattacat
    26881 tgcctatttt accagccatt ttttcagata agccagctaa attccctaat ccactttcta
    26941 taaaagtttt tattttcttt agtccttgac ctaaaatatc atcaaaaggt ttaacaatac
    27001 tcttcaaacc attgttaatg cttgtttgag ctcctgatat tatacctggt attgtattac
    27061 tcatttcttt agctaacccc atctgcttta cacttatgcc ttccaatgct tcatcaaaag
    27121 ttttataatt ttctcctacc agagtattaa gagattccat atttcctttt gctgcactta
    27181 agaaagctgc tgatacatct tgttcactac ttgtattcat atttaaagct ctaacatcta
    27241 ccatagcttg agttatatct ttttcttttt tgccaccagt aatattacta atcattttag
    27301 ctttagttcc cattgcaaca acatcttcag ggtcaaaagg cgtatcattt gccatattta
    27361 ctaaactgtc ataatactta tcaacatctt tttggctagt ttttccagta tttcttttca
    27421 ttacactttc tagaacaact ttctgatttt gttcctgacc aagtaagtca acaccctttt
    27481 tcacagcgac acctatagta gcaccatttg tcaaatcaga tatcttccct tttatcgaac
    27541 caagtattcc acttgccata tcctttacag ctattgcacc actccatacc ttacctgcaa
    27601 acaatctcaa tttgggaaat actttactta taatgctact tgccatatct tttatagcta
    27661 ttacaccact ccaaaaagta tttttaaaca acattaactt aggcaaaatt ctagccatag
    27721 cactacttac catatcttta agattaatac ttccttgata aggacgtttc tctactcgtt
    27781 ttaattttcc caagatacta cttatcactc tcttagtcat atcttttgct tttattacga
    27841 tattccaaat cttgccacca atctttttta aatttccaag taatttattt ttagtttttt
    27901 ctacttcatc acttttagct ttaaatatta atataatcat aatatttgaa ttaaactcat
    27961 ttttcttttg aatcaactta ttaaaataat caataatcat agctatagtt attgctagtc
    28021 tcataatact tccatttaaa cttccaataa ctcctgaaac agcatttatg ctagaactta
    28081 attttgccat agttgaactt acactactag cagtagtttg aacttttctt aacgaggaat
    28141 ttaaactgtt tactctacta cttgcacttc caatagaatt ttttatgcta ctagccatta
    28201 cattagtgga aagtgaaaca gccacagttg ctctcctaaa attatcagtt actgatataa
    28261 tttggttcat ttttggtgtg taattatctg taagatacat atttatacct tcttctctca
    28321 catttcccat tacataccac ctcctgtcaa aggacacact ccatatccat gtttcatttt
    28381 ttcaacttct tcctcaattt ctaaggcgaa aaaagcttca ataagcttta attcgccttt
    28441 atccattgca taaaaaaggg acggtcttat accttttttc ttccaataat agtacattat
    28501 tgtagtcttg gtatccgtcc ctattagttt tttacctctt ctatcactgc atttttacca
    28561 taacccataa catcttgaag tattctatat aggttggtaa tttcacctgg taataatagt
    28621 tttcttgcca attcttttgg tgttggagcc ttaaatttat ccattagttc cttattttta
    28681 aataaaagct ttccatccaa atcacaaaca ccttctataa ttgtaagcat ctgcatctcc
    28741 ggtaagtcta tatccgtttt catattttca tcaattttaa gacaagtatt ttgtatctct
    28801 tcatctttac ttattgttaa agctctacac attactttaa attcctgccc aaatatagtt
    28861 gataatctct taagttcaac tatagtactt ggtctttcta agttttctgt atctgcattt
    28921 aaaagtaaat ctactatgtt tatatttttt gataatccat tttcattcat tattatttgc
    28981 tcctttatca atcaaattca atcaactaac ttttttactt attaactacc taaatttatt
    29041 aagtatatat ctactaaata tcagtataaa accaaatata aaactaaatt atatcaagaa
    29101 aatcatactc agtaaatgtg aaaggtgctt ctatctctcc ttttactcca acctcccaat
    29161 cagctaaagt taaatcatca aatgctatat tttttactgc tattctttct gctccataag
    29221 aatctgggtc atttaattta gatatagcaa caaatctagg ttcagaacct ctctttatct
    29281 tttctccaat taacttgtgc attcttgaac taacatggta tagagttatt gaaccttttc
    29341 ctttatatcc catatacttt gtatcagtac ccatttgacc tgctattata atatcctctt
    29401 ttgtaaattc catctttgct tgaaacttct ttacttctgc tactttgttt ccatcaagcc
    29461 aaagttctcc ccaagtacca ctcattacat ttctagcttc catatttgcc atattaataa
    29521 tcctcctata tttctattga taaatctata tcttccatag catcaagtac ttttattttt
    29581 gcttttaaaa atactttaga acctgtgtta gcttctttta tttcttgtaa tgtcatataa
    29641 gataaatcta ctccttttga ttttaaatac gatttttgtg cttcaaaatc tatttcaaca
    29701 gtagaatcag attctataag tgctgacttt tctaattctt ctaaataact ttttatagct
    29761 actatcaata aacatttgtt gtcataactg ttagtaacct ttcctatata gtcatctatt
    29821 atcacctttc ttatgtcact atgtataata tctaaagtgt caactatttt tattttctgg
    29881 aacatttctc ctttttcttc tgttaactca gttaaagaat ttactcctct tgcaattctt
    29941 atagcccctg cttccttaat aagtataagc tctcctttat taacccttga ttctgcatca
    30001 actttcgtca tcttaggtat atcaactaca tcactaagct tagtataagt tactgattga
    30061 cttaaaggtg ttcctgctat aagtccagcc accctacttg taaactcatc aacactgtat
    30121 ttcttttctc caactaacac atcttctgta gtaaaattaa ttatcccttc atgatttcct
    30181 acaacttttc ctaatacagc tttaacctta accttatcta tatctctaag tttaattatc
    30241 caatttttta tagcagtctt atcagcttct actgcttttg gcatacatag ataattaaat
    30301 tccttagtct ctaaaaaatc taatgcagtt tgaatatctg cttctccttc tattacataa
    30361 actaataatt tatttggagt gttaacattt cccatcaaag ctaaatttat atactcttta
    30421 ttttcagcag ataaatcaac tggtatatcc tctttttcat gtatttcatt aagacctagt
    30481 gccttagcat ctttaagcac cattgcaatt attcctctag ctgaacgttc tttaacagtt
    30541 gtagctagct ccttaaatga tatgttgata cttggtaatc ctatagccat actatcactc
    30601 tcctaaattt aaattgattt cttccatatt ttcatatact acatcttctt catatacact
    30661 atccagataa tttagtgtaa ttgtaaagtt caatacataa attgactctt cttttttaat
    30721 actttgttct agctttgtta tatttaattt tctgtctgtt acctttatat ttctgttgaa
    30781 taacttattt aattcatctg aaatttcata cattctaata ctctttgact gtggttttgg
    30841 taagtatttt atagagataa caatatcttt attattcgta tttaacatta taggtgtaca
    30901 aataactgat agtatactta caaaaaaaca agacccttca gagccttgca cactttcatc
    30961 atcaataaaa atactttcag taaagttttt tttgagaatc tcaataattg tttctaatat
    31021 ttccttgtat ttaagcaaat aatcaccaca ctatcactaa atattcttgc ttaatctgat
    31081 aatcttacta taaatgatat aagtcatttt taaatataca tctcatttat tcctgtcaaa
    31141 tatcttctat atttttaggt aatattattc ttttcaattt atcacctcct tcaatatgga
    31201 tttctttcac aataacatta tctcacatat tttattgcct agtgttgcaa tagtgtatat
    31261 aaagtgttgt aaagttgtca tattttttat aatatctcac tcaatttaaa tatcattttt
    31321 ttctttaaat tgtagcaaga tgttctatct aagtgtagtt tcatagaaat atatgtcata
    31381 ttgttttttg tcttactatt ataaaaaaga ttaaaaaatt ccatttctat atcatttaga
    31441 catgttagag cattttctat cttctctttt tctatttcta tatcttcttt ttccattttt
    31501 aattttctta atctttcctc tctttttaac acctcatttt ccacagaaga acttatgtta
    31561 tatgtgttac tagtcttttc tgtatatacc attgctccac atccagagta ttcattctca
    31621 catcttttta tgtccaaatt aatattcttt atctttatat ccaaatactt ataattatgt
    31681 aatctttcat ctgtttcttt aaataatata tcttttttgt tttccatagt acttctcctt
    31741 ttttatatac ttattattag cttcaaattt acaataatct ctttagtgca cagtttttta
    31801 ctaatctcac aactttcaag aacatattgt tctataactg tataaaaaaa acttcttcta
    31861 tattttgttc taaaatacta gaaatcttct tagccataag aagaccagga ttttttatac
    31921 ctctttctat ttgagatata tagtcatttg taattccagc ctttctggca agttgaattt
    31981 gtgtcatacc tttgcatttt ctataatatt tcaagttatt tttaaacatt gttttatacc
    32041 tccattacca ttaagaatat tttgttcttc cacacttaga ttatatagaa tcatttgttc
    32101 tttatcaata gattttagaa aaaaatattc ttttatattt tattagtttt attttttaga
    32161 acaatttgtt ataatctata cataaggtgg tgttataatg tttgccaaaa gattaagaga
    32221 acttagaaag gaatttggat tgactcaaag agaacttgga gaaaaagtag gcgtttccca
    32281 aagagtctta gggtactatg aaacagaaaa cagatttcct gatgaacaca ttttaaacaa
    32341 attggctgat gtatttaatg tatcagttga ttaccttctt ggaagaacct tagttaagga
    32401 aaatattgat acagtagctg cacatagaaa aaatccacat gaagagttac ctgaagaagc
    32461 acaagaacaa cttaatgatt atatagaatt tttactaaat aaatataaaa aaaaataaac
    32521 catatgatga gcagtctatt tttctgctct tttaatataa tttaaaggca aacatacatt
    32581 ctattttagg gggattttta tgaataattt ggataagctt tttgaattag cttctcaaga
    32641 agagataata attcattata ccacttatat tgcaggtgat ttagaagggc tatatataaa
    32701 caaacatggt attaaaatta tatcattact cagtaattta aaacaaaact caaaaaaatt
    32761 gacatccatc ttagctgaag aacttggaca tcatttcact agccttggat actatgtatc
    32821 ctcttataac gattattata caaaaattat tatagataag tgcgaaaata aagccttaaa
    32881 atgggcttgt gagttcttaa ttactgaaga agatataata aacataatta attctggcat
    32941 cacttgcgtt tatgaaatgg cagacatact taatgtggat attactttct ttcaaaaaag
    33001 attagaattt ttatcattaa aaaaacagtc tttacaactt ggaaataata aatatttaat
    33061 attaactaat ttgccgtatt tctacatatt tgatccaatt tcataataaa aatagtagta
    33121 attttaactg gcttaacagt aaaatctata tatttatttt aacaaatttt ggtttctata
    33181 aattcaatat taatatataa taatgatatc atattattta aaattctaaa aacaaatata
    33241 tgcttttgtt aagaaaggtt gtttgatgaa tgtataaaga aaagttgttt gataattatt
    33301 ttaagttctt agcactatta ttttggccaa ttatgtggta taagtggata gtaatttcaa
    33361 atggtactct tgaaaatatg ttatttacca tatatgcaat cgtcgcaatt attttcatca
    33421 ttttatattc ggtgtttatg ataaaatata aagacattac acaaatagat ttcttttaca
    33481 ggataagtac tctacttgct tttatattta cactttttag ctttttaata tacccaaaga
    33541 gtttattttt cttgtattta aagattattt ttactggaat atacctttat tattcaatag
    33601 taaaaactct taaatttaaa gatgatgaag gtgtagtggg tattatgagc tcacttttat
    33661 taattgtaat aactttgttc tactaaattt aattctattt gacttaatat ggctttaaat
    33721 attagacatc tacattattt tctgatatta tttaagatgg tagtcttat
//
LOCUS       DAEPOZ010000013        33651 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.013, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000013 DAEPOZ010000000
VERSION     DAEPOZ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33651
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.013"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(278..976)
                     /locus_tag="KR440_000538"
     CDS             complement(278..976)
                     /locus_tag="KR440_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0697605.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKHHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
     gene            complement(1010..3712)
                     /locus_tag="KR440_000539"
     CDS             complement(1010..3712)
                     /locus_tag="KR440_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HBH0697606.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEVITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSSKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            complement(3890..5707)
                     /gene="ftsH"
                     /locus_tag="KR440_000540"
     CDS             complement(3890..5707)
                     /gene="ftsH"
                     /locus_tag="KR440_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBH0697607.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            complement(5738..6181)
                     /locus_tag="KR440_000541"
     CDS             complement(5738..6181)
                     /locus_tag="KR440_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0697608.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            complement(6672..7562)
                     /gene="metA"
                     /locus_tag="KR440_000542"
     CDS             complement(6672..7562)
                     /gene="metA"
                     /locus_tag="KR440_000542"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBH0697609.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            complement(7592..8857)
                     /locus_tag="KR440_000543"
     CDS             complement(7592..8857)
                     /locus_tag="KR440_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HBH0697610.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     regulatory      complement(8967..9069)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(9123..11780)
                     /locus_tag="KR440_000544"
     CDS             complement(9123..11780)
                     /locus_tag="KR440_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBH0697611.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNFSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFVPVFRNAFNLTVLNNL
                     EWLITIVLAIMSVIQVEVGKIIKRKKRKK"
     gene            complement(11908..12651)
                     /gene="yaaA"
                     /locus_tag="KR440_000545"
     CDS             complement(11908..12651)
                     /gene="yaaA"
                     /locus_tag="KR440_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBH0697612.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLRVGEFKNLYEYWGNRIYKELIKEKTAIIN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFNLEGYKYKENLSNNSEYIFVKE"
     gene            complement(12661..13125)
                     /gene="bcp"
                     /locus_tag="KR440_000546"
     CDS             complement(12661..13125)
                     /gene="bcp"
                     /locus_tag="KR440_000546"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBH0697613.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
     gene            complement(13477..15174)
                     /gene="ade"
                     /locus_tag="KR440_000547"
     CDS             complement(13477..15174)
                     /gene="ade"
                     /locus_tag="KR440_000547"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBH0697614.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNVEIDCSNKYISPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADP
                     HELVNVKGASAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNN
                     PRILGLGEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDH
                     ECITFDEVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDI
                     EKQGHIRWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLD
                     KMEVDSVYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEI
                     VPYQILTNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAIASLKGFGIKNGAIA
                     TTVAHDSHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFT
                     AEEVQEITDNMLEIARKMGVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
     gene            complement(15480..16634)
                     /locus_tag="KR440_000548"
     CDS             complement(15480..16634)
                     /locus_tag="KR440_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HBH0697615.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKFYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            complement(16879..17718)
                     /locus_tag="KR440_000549"
     CDS             complement(16879..17718)
                     /locus_tag="KR440_000549"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBH0697616.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFNNSKKYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            complement(17731..18327)
                     /locus_tag="KR440_000550"
     CDS             complement(17731..18327)
                     /locus_tag="KR440_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBH0697617.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            complement(18378..19028)
                     /locus_tag="KR440_000551"
     CDS             complement(18378..19028)
                     /locus_tag="KR440_000551"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBH0697618.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGVDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKSSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            complement(19074..20327)
                     /locus_tag="KR440_000552"
     CDS             complement(19074..20327)
                     /locus_tag="KR440_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBH0697619.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEKENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLREIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            complement(20351..20992)
                     /locus_tag="KR440_000553"
     CDS             complement(20351..20992)
                     /locus_tag="KR440_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBH0697620.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            complement(21194..21607)
                     /locus_tag="KR440_000554"
     CDS             complement(21194..21607)
                     /locus_tag="KR440_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HBH0697621.1"
                     /translation="MIELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDDIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNLYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            complement(21758..22099)
                     /locus_tag="KR440_000555"
     CDS             complement(21758..22099)
                     /locus_tag="KR440_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HBH0697622.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
     gene            complement(22393..23052)
                     /locus_tag="KR440_000556"
     CDS             complement(22393..23052)
                     /locus_tag="KR440_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBH0697623.1"
                     /translation="MLDAFITIVSSIIEIWSCKKVFDYTSQSKTDLKKLNMYFLFSVF
                     LIISSLYINIGANSRILICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSML
                     LVATVSNLDNMNVLLSGSLYRYEAIFLGKIMLGILLYVYKASFKNFKISKKEIYICIP
                     MIANLLSFFVLYDYIINLVKISPERRTAFLVIVLLLILSNISIFVLIHIILKIKIRKI
                     R"
     gene            complement(23583..23960)
                     /locus_tag="KR440_000557"
     CDS             complement(23583..23960)
                     /locus_tag="KR440_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH0697624.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
     gene            complement(24147..25118)
                     /locus_tag="KR440_000558"
     CDS             complement(24147..25118)
                     /locus_tag="KR440_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0697625.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFMNALWMYKFTGLFIKDKTK"
     gene            complement(25152..25349)
                     /locus_tag="KR440_000559"
     CDS             complement(25152..25349)
                     /locus_tag="KR440_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBH0697626.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAFFSPI"
     gene            complement(25417..26628)
                     /locus_tag="KR440_000560"
     CDS             complement(25417..26628)
                     /locus_tag="KR440_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HBH0697627.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSECGVSKYDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKHNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIVFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            26909..27511
                     /locus_tag="KR440_000561"
     CDS             26909..27511
                     /locus_tag="KR440_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH0697628.1"
                     /translation="MEICILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWAGKPVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEKHSRQFAKQIYYILDNSIEVDNC"
     gene            complement(27767..28738)
                     /locus_tag="KR440_000562"
     CDS             complement(27767..28738)
                     /locus_tag="KR440_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBH0697629.1"
                     /translation="MKKVISIGEALIDFIPNQNGCKLKNVSEFRRVAGGAPANVSVVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFMTGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(28764..30245)
                     /locus_tag="KR440_000563"
     CDS             complement(28764..30245)
                     /locus_tag="KR440_000563"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBH0697630.1"
                     /translation="MYKEPKYRTILESIKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGVIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSVLEIFI
                     NDGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            complement(30276..30359)
                     /locus_tag="KR440_000564"
                     /pseudo
     CDS             complement(30276..30359)
                     /locus_tag="KR440_000564"
                     /inference="COORDINATES: protein motif:HMM:NF012587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
     gene            complement(30582..31568)
                     /locus_tag="KR440_000565"
     CDS             complement(30582..31568)
                     /locus_tag="KR440_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBH0697631.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKELKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPMVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     ETSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKNIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            complement(31952..>33651)
                     /locus_tag="KR440_000566"
     CDS             complement(31952..>33651)
                     /locus_tag="KR440_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HBH0697632.1"
                     /translation="LVLTMFLFNNLTANAQIKEDSIIGIDRYETAGLIADRQTYDTVI
                     LVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLNRLDNKSLNVVKKVYIIGGY
                     NTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINEVSSVKEVMLTNGFVGEADA
                     MSIAPVVAKNKGAIILTDGKSVPFKTEGLNVYAIGGKSAINEDLVNETKATRIGGNDR
                     FETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEKPIVLVADGSNKGILKGAKS
                     ITKVGGIDANIYKQCLDMIKYNNMDVTPNILKHLTSVEGNEVSEILIESDNLGVVVEK
                     TNTNKFEFDYVSVTNEKNCTFSVYKENSSNNVKNGKLVVSAKKKIDKKDSDLQDMNGD
                     NMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYNIKGGATVDVNDGIANIVDN
                     NVTNPFNINTNSGISGVTAETISSEIKFRANNGIVSVTATNINGNISLFGSDNKGTFD
                     GIFKLNLKKEPSNLHLKLVGNGINKLPEGWSKDYILGNGQPIIEVKNNGINNISIGE"
BASE COUNT        10817 a         5807 c         3503 g        13524 t
ORIGIN      
        1 ttttacacta ttttaacaat tattatttaa tatttacatt aaatttatta taaggtaagt
       61 gttaaataca tttattattt aaccaaagtt tttattacag attacactat atacttattt
      121 taaaacatat ttcatattat tgatactaaa aagctaaaaa ataaaatgca tgtgctaaac
      181 tgacaacaag cacatacact tcattcttat tttaactaaa ctatataatt ttaatatata
      241 ttaataagca cataatctaa taattaatat attacaacta ttcatcattt aatctgtatc
      301 ctacaccaac ttctgtaatt atatatcttg ggtttgcagg ttgtttttct atttttcgtc
      361 ttaatgttgc cataaagacc cttaaagact tagtctcatt ccctataaca ctaccccata
      421 tttcttttat tataaaatta tgagtaagca cttttccatg atgctttgca agtaatgaaa
      481 gaatattata ttcaataggt gtcaaatgga cttcaacatc atcaacaata acttttcttt
      541 tatcaaaatc aatcaataat ttatctactt caaacgtaga tttaacatca tctttttgtt
      601 gagcttctac ttgtgaatgt ctaaatgcga ctcttactct tgctaataac tctactatgg
      661 agaatggttt tgttaagtaa tcatctgccc ctgcatcaaa tacttctacc ttatctctat
      721 cttgctctct agctgaaact acaattattg gtatatttga aaattgtctg atagctttta
      781 ttacctctat tccatccata tcttctaagc ctaaatctaa aagaacaaca tctggagaat
      841 atgagacagc aatagatata ccttcttttc cagttatagc ctccttgaca tcataatcct
      901 gtgttaaaag tgcagtagat ataaattttc ttattgtctt atcatcctct actaacaata
      961 taagtttttt actcatttct tcctccaaaa ctaattttaa aatttaaaac tattcatttt
     1021 ccttaggcaa actaaactta aaagttgaac ccttatcttt attgttttct accataattt
     1081 cacctttatg agcttgaatt atagatttac aaattgataa tccaagtcca acaccctttc
     1141 tggaatcttt agctccttct tctccagtaa aaaatctgtc aaagatatgc tttttaagtt
     1201 cctttgatat accaggacca ttatcagata cttcaaacca aacataatca tctttctcgt
     1261 atacatttat ctctatctta caatcttcct ttgaatattt caaagaatta tctattaaat
     1321 taattaaaac ttgttctatt aattttgcat ccataggaac aaataatatc tgttctggta
     1381 tattaatatc tataagtgca ttttcaattc ttttttttat tttttgtaac gcttctgata
     1441 taatttcctc aaccagttca gaatgttttt taacttccaa tttcccttca tctatcttgg
     1501 tcatactaag taaattttca acaagtctta ttaaccactg agtatcttca tatacaccac
     1561 taagtaattc atctattata tcttgaccta tagtaccctt gttttttata attgtactta
     1621 ctgcaccaga tattcctgct agtggagttc taaggtcatg cgaaactgct cttaaaagat
     1681 tacttcttaa tctttccctt tcaatctcaa gattggtatt ttctttagct tctgacaaga
     1741 tttctctatc aagcgctatt gccatttgag caattattgt ttcaattaag cttatatctt
     1801 ctgtatccaa ttttgaatca atacatgata ttcctactac tcctaaagtt aaattcattc
     1861 ctacaattgg tatatagtat ccctttgaac caggaagtgt atccgtgtca tttcctgcca
     1921 ctgttgaatt ggataaaacc caacttgcaa cagctttttc atcttcacta tccaaatcat
     1981 ttttatcctt attattagta ttttttttgt aaagttttga tttattatgc ttatctacat
     2041 ttaaataaca aattacatct ctacttaagc taagagacaa aagttctata cctttgttta
     2101 ctatatcatc tttacttgat aatcttaaaa atcccttaat tatttgatat atcatttttg
     2161 tattttcttc tcttttagca gcagtttctg cttcatgctg tatcttagac gtaagagtgc
     2221 tagttattat acctactatt gagaatacaa ccagtgttac tatatagttt gaatctgcta
     2281 tagataatgt atataatggt gctgtaaaaa aataatttaa aagaactata ttaaatacag
     2341 aacttattat acctgtacta taccctttag tccacataga aactaaaact acacccaaca
     2401 tatatattaa caatatattt tctcttataa atcccatgct ttgtactgca tatgctaaaa
     2461 tacttatcat caaagtaata aatataagtt ttataaaatc aactttgcta attttaaatt
     2521 tagattttat tgatgattta tcctttttag gtttgtattt aacatctgag gcaaatttat
     2581 atggaattat atgtatatct atataatcaa cttcatcaat taatttgtca actatatctt
     2641 ttttaaatct tttactaaat cttttattac ttgaatgatt tctacctatt acaatttttg
     2701 ttacatttct cagttttgca attctaagta tttgctctat tatattttca ccatgtaaaa
     2761 caattatttc tccacctaat ttcttagcta attttaggtt attttgtagt tgtttttgtt
     2821 catcaaatgg aagtcttgct gtgtttggag tttcaacata taaagctatc cattttgcaa
     2881 atgtactgtc tgatattctg gatgcagttc taataacttt tgctgaagat ggagatggac
     2941 ttatacatgc aagaagtgta tctgatgtag gtattacagt tacatcaccc tttgatagtc
     3001 tggtcatttg aacttcttta ttaactctct ctgctgtttt tctaagtgcg atttctctaa
     3061 gtgcaaccag gttatctttt ataaaaaaat tattttttgc tctagtagct tgttctttct
     3121 tatatatctt accttcattg aacctttcaa gaagtacatc aggttcaaca tctataagtt
     3181 ctaactgtgt atttgtatcc aaaaacttat ctggtatagt ctctttaact gatacgtttg
     3241 taattacctc aacaatgtca tttagacttt ctaaatgttg tacatttaca gtagtgtaga
     3301 catttatgcc tgctaacagc aattcttcta tatcttgcca tctttttgta tgccttaacc
     3361 cagcaacatt agaatgggca aattcatcta ctaatataac ttctggtttt cttgctaaag
     3421 cagcatctaa atcaaattct tttagtttta tagatttata ttctatctcc tttacaggaa
     3481 gaatttccaa gccatcaagt agagccaatg tttctggtct agtgtgtggt tcaatatatc
     3541 caactactac atctacaccc acttttttta aattttgtgc agattccaac atagagtagg
     3601 tttttcctac tccagctgca taaccaaaga atatttttaa ttgacctcta cttagttttt
     3661 cttcttcatt tttaattttt tctaatagaa tatcaggatt aggtctatcc attaaaaaac
     3721 ctccttacac cattcattac tattataata tcaattccga tataaaattg tagttaagat
     3781 gtatttttaa tgattaagat tatgtaaaga tttaaatatg aatttaaaaa atttagttgt
     3841 aaaataaaaa tgcctaacaa taggcatttt tatcttgtgt ataatcattt tagtttaagt
     3901 taagtatttc cataaactca tcacctgtaa tagtttcttt ttctaataaa taattagcaa
     3961 gttcatgcaa tttatcaata ttattttcta agatttcaat tgcatttttg tgacaagcct
     4021 ttactatttt aaatacctct tcatctattt tagcagcagt gtcggaagaa caagcaagag
     4081 aagtatctcc tcctaaatat tgattgctta cagtttcaag agcgaccata tcaaactctt
     4141 cactcatacc aaggcgagta accatagctc ttgcaatttt agtagcttgt tctatatcat
     4201 ttgatgctcc tgatgtacaa gtgttaaaga taacctcttc agcagcacga ccacctgtaa
     4261 aagtagttat tttatcaatt gcttcttctt tagacataag tacactctca tcttcagcaa
     4321 cttgcatagt atatcccaat gctcctgaag ttctaggtat tatagtaatc ttatggacag
     4381 gtgcagaatg tttttgcata gcagcaacaa gtgcatgacc tatttcatga tatgctataa
     4441 ttttcttttc cttatctgaa attacagctc ctttacgttg atacccagca attacagttt
     4501 caacagattc atcaaaatcc tgttgttcta ctatatctct gccagactta actgctaaaa
     4561 gtgctgcttc gtttacaata tttgcaagtt ctgcacctga tgctcctgat gtagaccttg
     4621 caattgcatt ataatctata tctgagctta catttacatt ttgagcatgt acctttaata
     4681 tagcttcacg tcctccaagg tctggaagtt caactggtat acgtctatca aatcttcctg
     4741 gacgaagtaa tgctttgtca agactgtcag gtctatttgt agcagcaagt attactactc
     4801 caattccacc atcaaaacca tccatctcac ttaacaactg atttaaagtt tgttcacgtt
     4861 catcatttcc acctaatcca cttccattat cacgcttttt accaatagta tcaatctcat
     4921 ctataaacac tatacatgga gctttttctt ttgcttgttt aaataaatca cgaatcctag
     4981 aagctcccat tcctacaaac atctcaacaa actcagaacc agatattgag aaaaatggta
     5041 catttgcttc acctgctaca gcttttgcaa ggagtgtttt tcctgtacct ggaggtccta
     5101 ctaaaagtgc tcctttaggc atttgtgctc ctatcttttt atacttttct ggattgtgta
     5161 agaaatctac tatttcagtt aaagcttctt ttgcctcatc ttgaccagct acatcattaa
     5221 atctttttcc actttgagca gatacataca ttttggcgtt gctttttcca aattgcatag
     5281 cattaccacc cattcgcttt tgcatagact tcatcattaa gttaccaaat agcataagaa
     5341 tacctatagg tattatccat gtaagtaaaa tacttaaaag aggtgaattt tccttaggaa
     5401 taaccttagt aaattctact tttgcatttt ttagtctatc taccaattct gggtcatcca
     5461 ttctaccagt tatgtatata ttcttatcct tgctactaga tggatttatt gctattctat
     5521 tattttctat ctcaacagat ttaacctttc ctgtatccaa cattgtgaga aactttccat
     5581 agtcaatctc aaccattttt ttatccatta aacttggaaa tattaaagca tttaaaaaca
     5641 ttactactat tattataact gtataataat agagtgctga ccttttttgg ggtttatgtt
     5701 catccataat tttattctcc tttaaattat ttttatgtta tttgcaatca atgtaagtta
     5761 aaagtttatt taatttctct aaaccttcta ttatatcttc catatcttgc aatttttcac
     5821 tggtcatatt ttcaaaataa ctatcaaaaa tactacttga aatatgttct tgtatatcca
     5881 atgatttatt tgtaactttt acttttacaa ttcgttggtc ttctatatct cttatgcgat
     5941 ttaaaaagcc atttttttca agcctttgac agattactga tacattacta tttgtcatgt
     6001 ttaagttctt ccctaaatcg cttatttttt tctctccatg ttcacataac tcagataaaa
     6061 tcaaagcttg cactgctgta ataccaagtg gctgataaaa atcacttaat aaagttgtaa
     6121 atttcatatt tatatctcta gtcaactcta gtagctcttt cttaaaactc atctcatcca
     6181 tacaaacgct ccctacgaaa atactttatt tcaaatttag gctgaagtgc atactgtaaa
     6241 taaatactta atactataat gtttaatgta ttaaatatta tagtattaag tatttattgt
     6301 caattacaat attgtaactt caattggaat tttgttatct tattgatatt taattttata
     6361 caggatattt ttaataaaat ttaataaaaa atcgatatat atattttaca ctgatttctc
     6421 cctttttaat ctcttttaaa agttaaaata caaatttatt gatactataa aggtttagtc
     6481 tcccattatc gcaccatata taaaattaaa accacctcta tctacataca cctaaagtga
     6541 ttttcttaaa atataaaact actattataa ggttaaattt aaaaaaaata aggtataaag
     6601 tatcattaag attaacttta tacctataaa tttctatatt tttttagtta acatttatat
     6661 actcttaaaa tttattgtat caaatagttt tttatccaat ttctaaacaa caattctgaa
     6721 tgttttttcc attttacagt tggctcttct gaagggtcat cattaatata gtaattttga
     6781 ggtatagata ttttatctcc tttatttata tccctcttat attctttatc aagagtgtat
     6841 ttgtcatact cgctatgacc cattacgaac acatctcttg aatttgtaat tatataggga
     6901 ccagcttctt tagaatgact cacaatgctc aactctttat tctctttcaa agcttcaata
     6961 tttacagtag tatgtcttga atgtggagca aaaaattcat tttcaaatcc atctactatt
     7021 ttactatctt tatctacctt atgcttaaat actccaaaac atttttgact tagtggaagt
     7081 ttttttacat tgtagtattt gtaaagactt gcttgagctg cccaacatat atataatgta
     7141 gatttagttt ttctcttaga ataatccata atttcttcta gttcattcca ataatcaact
     7201 tcttcaaact ccatttgttc aactggagct cctgttatta taaagccatc aaaatactct
     7261 cctttggctt catcaaatgt tttataaaat tctttaacat attcattaca acttctttta
     7321 ctttctcttg ttgaaatttt aataaattca acagacacgt tgaatcctgt tttatctatc
     7381 cttcttagta aatctaattc tgtatctatc tttataggca ttagatttag tatagctatt
     7441 ttagtgtcta tgttttcttc atactttaat tcctttttaa aatcttcttt ataaattaca
     7501 tctattcctt catctaataa tttattaata acaggtaatc cctttggtag tattaaagcc
     7561 atatcttttc acttctcttt ctcttagaat attagaaatt taaagcttga tttaaatcag
     7621 ctattaaatc ctctacattt tctattccaa cagataatct tattagtgaa ggcaatactc
     7681 cagatgcaat taattgttct tctgttaatt gtctatgagt agttgaagct ggatgtataa
     7741 cacaagttct tacatctgaa acatgtgtaa ccaaagatgc tatctgtaat ttttccacaa
     7801 attttttggc attttctaac ccacctctta ctccaaatga taaaacacca ctacatcctc
     7861 ttgataaata ctttttggca ttctcataat acttgttatc ttcaagtcct gggtaattaa
     7921 tccaatctac attttcatgt ttttctaaaa atctagctat tttaagtgca ttttcactat
     7981 gtctctccat tctaagatgt agtgtttcaa catttaaatt agtaagatac gcattgaatg
     8041 ggcttaaaca atttccatag tctctaagca actgaactct tgctttagtt gcatatgcgg
     8101 catttccaaa tttttgagta tagcttattc catgatatgt tgggtctggc tcaactaatt
     8161 ctggaaattt tccattatcc cagttaaagt ttccaccatc cactataatt cctccaacac
     8221 ttgaagcatg cccatctaaa tattttgtgg tagaatgagt aactatattt gctccatact
     8281 ttaaagggtt acaaagcact ggagatgcta atgaattatc aacaataaat ggaacattaa
     8341 ttctcttagc tacatttgct attttttcaa aatctatgac ttcaagagtc ggatttgcaa
     8401 gtgtttctgc aaatacaacc ttagtatttt cctttgcaag ttctactatc tcatcttcac
     8461 ttgaatctaa gtcaaacgat attaaatcta taccaaattt tttaagacta ggtcctagta
     8521 aattgaatgt tcctccatat acttttgaag aacaaagtat actatctcct gatttacata
     8581 tatttaaaac tgccaacata tttgcagatt gccctgatga tacagctaca gaagatactc
     8641 caccctctag taaacttatt ttttcttcaa aagcttgaat agtaggattg cttattcttg
     8701 aatatatatg tccatcaact tttaaatcaa ataattcagc aagttggtct atactgctat
     8761 atttaaaagt tgtactttga tataaaggta atactcttgg ttctccatta cctggtttat
     8821 aatttccttg cacacatatt gtttctttat tatacattct tgtatccccc ttagatttta
     8881 gtaaaaaaaa ttaaactatt tatcagatga gagttaaaat aaataaagcc tgagtatata
     8941 actcaggctc tgtataagct taattatact catctctcag ctttcgccgc aggatttagc
     9001 accaatgtat ataaatatac tggttgccgg gtttcatagg gccagtccct ccacctctct
     9061 ggataagatt tatttaattt ttctatgttt tatatcttat atatttttcc taattttgtc
     9121 aactattttt ttcttttttt tcttttaata attttcccaa cctctacttg aattacagac
     9181 attatggcta aaacaatcgt tataagccac tctaagttat ttaaaactgt taagttaaaa
     9241 gcatttctaa aaacaggtac aaacagtatt actgctatta aaaatgcaga agttaaaaat
     9301 gagattaaaa gaaatggatt tttaccatta tttctattaa acattgaatc tgtatttgaa
     9361 cgctgactaa atgctcgtaa catctgtgaa aatgctaata cacaaaatgc cattgtttga
     9421 cctacagaat gattttctat attaagacca attttatatg ctaaaatagt cattattgcc
     9481 acaaatatac cttgggtaac aactctttgt ataagatttt tttcaaacaa agtggttgat
     9541 ttcactggct ggtgcttcat tatatttctg cttggtgggt caactccaag agcaagggct
     9601 ggaagcgtag cagttgcaag gtttacccat agaatatgaa cagcaagaag cggagtatcc
     9661 caatttaata atattgctac aaacaacgta agtatctctg ctatattacc aacaagcaaa
     9721 aattgtataa ctttttgtat gtttctatat acacgtctac cttcttttat tgcataagca
     9781 attgttgtaa aactatcgtc cattaaaatc atgtcagaag aatcttttgc aacatctgta
     9841 ccacttatac ccatggctat accaatatca gcagacttta aggcaggaga gtcatttaca
     9901 ccatccccag tcattgctgg tacttctcca attcttttta gagattgtat aattctaagt
     9961 ttatcaaacg gggacacacg agcaaatact gtagtatttt taacagcttc atctagttca
    10021 tcatcactaa aattatcaag ttcatcacca gatatcacag tattttcttc actccatatt
    10081 cctaactcat gagcgattgt tgtggcagta actttatggt cacctgttat cattatggtt
    10141 cttatacctg cttctttaca agtacttact gcatcaatta cctcagttct tggtgggtca
    10201 atcataccaa ccatacctat aaatgtcaaa tcatattcta tattttcact atcttctttt
    10261 ggtatagatg aaatctcacg cttagcaaaa ccaagtacac gcaatgcatc tttagacatg
    10321 ttaagacata attcgtgtat attcttttta tccacttcgg taatgtttct ttcacctttt
    10381 gaagttaata ttttagtaca taggtcgaga atttcgtcta ctgctccttt agtataagca
    10441 ataatctttt catctatatt atgaacagtt gtcatacatt ttctatctga atcaaaaggc
    10501 tgttcaaata cacgtggata ttcttcttca agagcttctt gatctattcc aaattttttg
    10561 gccataaaaa ttaatgcgcc ctctgttggg tcacctaata tttcatctgt tttatctgga
    10621 ttaatacatg catcattaca aagtgcacca gcataaacaa tctctttata cactaatgga
    10681 tgtttttcag atgcagtatc tattgatgtc acttttccag attcaaaatc tccattgaca
    10741 gcaatatgtg taacagtcat tttatttaag gttaatgtac cagttttatc acaacatatt
    10801 actgtagctc ctcctaatgt ttcaacagca ggtaattttc taattaaagc atttctttta
    10861 gccatacgct gtacgcctaa agccattaca atagtagctg ttgcaggtaa tccctctgga
    10921 ataattgata tggcaaggga aattgcaatc ataaacattg gcaagagagg tctctgataa
    10981 aatgcaccta ttgcaaatat aacaacgcat acaataatac ctatgacact taatgtcttt
    11041 ccaacagtat taagtttacg ttttatagga gtatcaaaat catcttgtga atctaaaaga
    11101 tgtgctatgt tacccacttc tgtatccata ccagttgcta ctacaacacc tacacctcta
    11161 ccatatgtga ctattgaaga tgtatacgcc atattgctac ggtctcctaa tacacattct
    11221 ttaggtaaga ttgtatctgc atctttttct gatggaacag attctcctgt taaagaagcc
    11281 tcttgaattt ttaaacttga tgtttctatt aaacgaaggt ctgctggaac catagctcca
    11341 tcttctaaaa aaacaatatc tcctactaca agattacttg ctggaataac attttcttca
    11401 ccatcacgca taacacgagc tgtaggtgag ttcatatttt ttaatgcttc taatgatgat
    11461 tctgcttttt tctcttgaac aattccaata actgcattta ctgttacaat tattagtatt
    11521 acaaaagctt ctgtaaattc gccaaatatc aaagataata ttgctgctcc tattagtatc
    11581 ataaccatag catctgtaat ttgactccaa agcatttgta aagttgttct aggtggtttt
    11641 tttctaagtt catttaagcc attctcttta agccgttttt ccgcctcggt atcggtgagc
    11701 ccctcctctg atgaatctag actttctaaa atttcatgcg ttgctcttga atgccaaggc
    11761 atctgtttgt gattttccat tttggaagac cactcctttc tttttttaat ttaagaataa
    11821 ttatatttta aaatcaagtt caattcaata cacactaagt atattttacc taaatttaac
    11881 aatgccattt aatatttaac aaaacttcta ctcttttaca aatatgtatt cactattgtt
    11941 agataagttt tctttatact tataaccttc taaattaaac tttttcagtt cttcaatatc
    12001 tcttatccta ttttttacaa tgtattttgt catcatacct ctagctttct ttgcttgtgt
    12061 agattccact ttcaatatgc catttttatt tactttaaaa gtacatgtaa tatatgtatc
    12121 actatcttta ataaattttt ctatggactt gctgtattca tttgatgaaa gattaatgat
    12181 agctgttttt tctttgatta gttctttata tatacgattt ccccaatatt cgtataaatt
    12241 cttaaactca cctactcgta attttgtctg catttctaat ctatattcat atatactatc
    12301 atatggtttt acaacaccat acaatccact tatgattcga atacagctat ttgcaaactc
    12361 ttcatctttc cttgtaaaat tttctgcttc tatattttta tattgtattc catcataagt
    12421 taagattgca gggtttccta atctatcaaa tttaatgctt tcaaatctat ttttatttaa
    12481 tacagctaat ttttcattaa ttttcatttt atttttaaaa tcttctatac caaatgtctt
    12541 tatattttcc actatttctt ttgtgttatc tataaaacaa ggcaaactca aatctctatc
    12601 aaatacttct atattcttca tatttttagc tggtgataac attataatca taaattctta
    12661 ttactcctgt ttttccaaat attcaagaat ctcttgtgca tttgtgtctg gttttgcttt
    12721 ttcaaagact ttttcaataa ttccattttc atctataata tatgtcgcac gtacaactcc
    12781 caatgcagtt tttccataca tttttttctc tttccataca tcaaaagctt ttatagcaac
    12841 taagtcaggg tcagaaagta aaataaaagg aagttcatgc ttttctgcaa atttttgatg
    12901 tgattttata ctatctttac ttatcccaat aacttgtata tcttcctttt taaatccatc
    12961 atatgcattt ctaaaagcac aagcttgacg tgtacatcct ggtgtattat ctttaggata
    13021 aaaatatact actacttttt tgcctttaaa atctgacata cttaccttat ttccatcttt
    13081 atcttctagt gtaaattctg gtgcttttgt tcctatactc aacataaata tctctccttt
    13141 tgtttttatt aataatttta tactatgctt atattattac cctataaatc tatatataaa
    13201 taaaatattt tattttttat gtataaagtt tcataaatat ataattttca tgtatttata
    13261 ttctaaaata gactgaaaat agttttatta agatatctat gcttaaaaca aggttaaatg
    13321 atagattaat taaaataagg atatctaaaa ataaaatttt tagttcatta gatgctcttt
    13381 tttgtatgaa atcaaatata ttttcatcat ttcaacaata aaaagagact tatccctatt
    13441 ttgagaaagc ctcatttatt attactttta tattatctat ataaatttaa attcctcaac
    13501 atcaaataga cctaaatctg taagtcttat ttgtggaaca actggaagtg ccataaaaga
    13561 aagtgtaata aaagggtcta catactctgg aactcccatt tttcttgcta tctccaacat
    13621 attatcagta atttcttgta cttcttcagc tgtaaaagta ctaattaatc cagctacttg
    13681 aagacttagg tgtgcaagaa cttttccatt tgatacaatt acataaccac cttgtatttc
    13741 ctttaagtaa tttatagcta ctagaatatc atcatcatta tcccctgcaa ctataatatt
    13801 atgagaatca tgggccacag tagtagcaat tgctccattt tttatgccaa atccttttag
    13861 tgaagcaatt gctacatttc cagtcattct atgtctctca acaacacaaa gttttgaata
    13921 ctctttattt ggaacaaaat atccatctgc acatggtaaa gattcatata ccttatttgt
    13981 caaaatttga taaggtacaa tctccattac ataatttttt tcacttactt ttaactgtat
    14041 tttttcttta ttaatatact taaacttgac tgtattaagc aattctttat ctttaatctc
    14101 ataattgtaa ttagaaaaca tctcttcgtt taccaaatta ccatctttat atacactatc
    14161 cacttccatt ttgtctaaat cattcaatac aacaatatct gccttataac ccgcacctat
    14221 tgctccaatt ttccttaacc catatgcttt tgcagaatta taagttgcaa cttttatagc
    14281 cctaacaggt tccataccta aatcaatcgc acattttata ttccatctta tgtgaccttg
    14341 tttttctata tcatctaagt gcttatcatc tgtgcaaaac ataaattctt caattggcaa
    14401 gttatgcttt agcataccac ttactataga ctttaaattt tttgctgcgc ttccttctct
    14461 tattaaaatt tttaatcctg ctctcaattt ttcatatact tcatcaaaag ttatacattc
    14521 atggtcattt tctattcctg cacatacata tgtctgaagt gatttaccat ttatattagg
    14581 tgcatgtcca tctacaactt tatttttaaa aagctctagt ttatccaaga tttcattttc
    14641 agaatttata acatcattaa aacacattac ctctcctaaa cctaaaattc taggattatt
    14701 tacataactc tccatgtctt ttgcactaaa tttaccaact ccattagttt caaaacttgt
    14761 tgcaggtacg gatgaaggca ccataatata tacattaagt ggtatatctt ttgtcgattc
    14821 taataaaaaa tctattgcac tagctccttt tacattgacc aactcatgtg ggtcggctat
    14881 gatagttgtc gtacctgatt ttaataattt tttcgataat tcaattggca ttaccattgt
    14941 agattcaata tgcatatgtg catcaataaa gccaggtgat atatatttat tgctacaatc
    15001 aatttctaca ttgcccttgt aatcgccaat gccaacgata gtgtctccta ctattgctac
    15061 atctcctgtt attaactctt ctgtaaatac atttatgatt gtcccatttt ttagtacaat
    15121 gtcagctttt tctcttccac ttgaaacatc acactgtctt tttaaattac ccatttttac
    15181 aactcctcct aagtttttta tatcaatata ctggctgtat tacgtattat agtttaattt
    15241 attaatatat attatatatg agttttgata gaaagtatca cttgaaattt gttccataat
    15301 tcctttaatt aaatagagag aacatgagga ataatattat ctcccatgtt ctcattaata
    15361 tttaagcaat aatttaattc atattgaatt ttttattttt atatttaatc ttcactattc
    15421 ttataaataa atttatgtgc tttattattt ttttaatact tctttattcg taattatatt
    15481 taacctaaaa atgcataaac tatcatacct acaagtattg ctggtatagt agtaataatc
    15541 atagcccata aagaagattt tttatccatt gctaaaagtg ggaaaagtgc atctccatct
    15601 tgagaaatag agttggctat cactgcacca agtggtatag ttcccttcaa ataaaatgac
    15661 ataagcacaa tttgtatccc acaacctgga ataagtccaa gacctgcacc tataaataca
    15721 cttattacac ctgttgataa tactagttta actaagtatt catctccacc taaaattaat
    15781 attatcaaat cgtaaataag atatgcaata aaaatccatg taattacaaa tgatatttct
    15841 ccaacagaat ggataagcat ttctcgcaaa gaaatctttt tattttcttc ctctttttga
    15901 ttagaatttt taaatacctt tttaaaacac cacatataaa ttattgaaaa taatattcca
    15961 atcactgcta ttacttcttc caatgaatgt aacttctcaa ttataggaag attaagtcca
    16021 gagtgagcaa gtgacataaa tataaaaccg attactataa ttcctatata aaatttatat
    16081 ccctttccat gtgttattat aaatgcaagt ctatttgtat ttccatgaga tttagctaga
    16141 gtgtctaaaa ttatttcatc atgattatgt tgttctttat tttctttagg gttattttta
    16201 cttatatcat atttatctga ttttctacgg ttttttaacc ctaatttttc ttctagttta
    16261 aagtaatcca ctaattgtcc cgttattatt cctgtaattg tactaactat agtgacaaaa
    16321 aaatacattt taatatttga tgaaataagt acaaatgctg catctcccat actagctatt
    16381 aaacttgcaa ttattgctcc aaagcttaat tttccattta tatacaatgg cattattgcc
    16441 agacttccgc cacaacctgg tatagctcca attaaagcac ctattaaggg ttgaaatttt
    16501 ctgttttttg atatgatatt agtaaaattt ccactagttt tataattaat atatccaaat
    16561 aacaaaataa aaaatccaat catagaacct acatgtaaaa acgcctcttc agaagcggat
    16621 atcaacattt ccattaattt acctcctaga ctaattttgc aacaattata ttatattcta
    16681 ttttctattg ataatcaata tcaatatttc ttttgaattt ttaatattaa aaagtgcgca
    16741 tcttatactc ttagtttttc atcttaagat acacactttt tgatattagg ttatctattt
    16801 accacatgta atatgaaatc aaaggtggat attatgataa attataacaa tgtccaccta
    16861 tttttattac ctatcaaatt ataaattttc aaggaatgtt acaacttctt ctattatcca
    16921 attaggtgta gatgcccctg ctgtaatacc caaattttta tattttttaa gcgtatttac
    16981 atccaattca tctttagtct ctattgcaaa agttggtact aagtcttcac aaacctttac
    17041 taacttttga gtatttgaac tgtgtttacc tcctataact atcatacaat caacttcttt
    17101 agctaactct ttagctgatt cttgtcttgt ttttgtagct gaacaaattg tatcattaaa
    17161 tactatttct tctaatttat ctgatagttt attaactata cttatataca actctggatt
    17221 tattgtagtc tgtgcaacaa gacaatattt tttggagtta ttaaattcca tattttccat
    17281 ttgttctaag gtctttatta ttatagctga attttcacac catccattta tacctataac
    17341 ttctggatgc ttcatatctc caataactat tatttcatag cctttattat gatattcact
    17401 tactactgta tgtattttct ttacaaatgg acatgtagta tccacaattt ttaatttttt
    17461 atctttggct tccttatata tattttcagg aactccatga gaccttatta tcatattttc
    17521 atgatttggt attgtatcaa tttcattcac agtcttaaga cctttttctt catattttgc
    17581 tactgcttgc ttgttatgaa taagaggacc taaagcatat atgccagaat catttttttc
    17641 aacttcatcc catgccattt tcattgctct ttttactcca aaacaaaagc cagcattttt
    17701 tgctattttt atgttcataa ctttaccccc ttattttgaa tatttaaagt aattttgttt
    17761 tatcacttct aaaatctctt gtgatatcct ttcatggtca tcattactca atttttcctt
    17821 aaaatgttct tcaaatgata ttggttctcc aatatataca attactttac taaaaagttt
    17881 atatttagat attattgata ctggaacaac tttagattta ctttttattg taaatacaga
    17941 aagacctgcc ttagccttac caaaagaatc tccttttatt ctagtaccct ctggaaatat
    18001 tcctaataca taaccatctc taactgacct taaaatagtt tttattgtag atacatctgg
    18061 tttttctcta tttataggca taacatgcaa tttttttaat ataaatccca atggctttac
    18121 tttaaacaat tctttctttg caattgcagc tatttcccta ttttctatgg cagcagccaa
    18181 gaatataggg tcaagattcg atttatgatt tgatgctatt actatatttc ctctatctgg
    18241 aatattctct gcacctataa cttcatattt aaaaaatatt ttagaaaaac ccttaaaaat
    18301 atttattaca aatctataaa aattcacgtt tatctccctt caacactaca tttattttca
    18361 ttatgatgca taaaaaacta aagatttatt ttagatacaa cttcttctac aacttcttct
    18421 atagtttttc ctgtagtatc tatttctagt gcatcattag cttttaccaa tggtgcaaat
    18481 tctctatttg aatcaatttc atctcgtctt attatatcct caattacttc ttctaaagta
    18541 gtattatatc ctttattaca aagttcttta tagcgtctat ttcctctttc ttctggagta
    18601 gctactaaaa agaatttgta atctgcatct gggaaaacat atgaacctat atctctacca
    18661 tctagtatta ctgaactttt cattcctatt tttcgttgaa cttcaaccat caactgtctt
    18721 acttctttta tttttgctac attagaaaca ttattgctta cttctattgt tcttatttct
    18781 tcatttataa cttctttgtc caaatatata ttgttatctt taaaatctat atccgaattt
    18841 tcagctattt gaataacttc tttttcatta ttgacatcaa caccactttt taaacactta
    18901 taagtaactg ctctgtacat agcacctgta tctatataat ttatatttag cttttttgct
    18961 actatcttgg ctattgtact ttttccagct cctgctggac catctacagc tataactaaa
    19021 ttacccatat atacacctct atttattcat atttaattat taaaaaatta tatttataca
    19081 cttatacccg caagatatcc agttgaaaat gctatttgta aattatatcc accagtttct
    19141 gcatctatat caattacttc acctgtaaaa aataagttct taactaattt agattccata
    19201 gtagaagaat taatttcttt aactgaaata ccaccagaag taaccatacc agtgtttata
    19261 cctgtagtcc cattacaagt tagcttcata ttttttatat actcaataat tctaatttca
    19321 tctgccttag acaaatcact tgccttttta tcaactaaag ataatagaga aaaaatctct
    19381 cttaagaaat tttgaggtag tatttgctta agattattta gaacattttt atttggaaat
    19441 gctctgatta tactagagat ttcatcagta cttaaatttg gtaagaaatc tatatttaat
    19501 tctacctttt ccttttcaat aatcttgtta atataagaag acattataag aacacaaggt
    19561 cctgttatcc caaaatgggc aaatagcatg tctccagatt ttgatatttt ccttttctta
    19621 atcttacaag atatttccac attcttcatg gatattcctt gtaaatcttt aatccatttt
    19681 tcctctattg taagtggaac taaagcagga tatagtttgt ttaatgtatg tccatgcttt
    19741 ttaagaatct tatacattga accatctgaa cccgtctttg aataactcac tcctccagtt
    19801 gatataataa ccttgtctcc tagtatctta tctccattat ctaaaataat cccttttatt
    19861 agatactttg acttgtcctt attagaatca tcttttagag caatttcttc agttataata
    19921 tctataactt tcttattata cattatcttt acattgtgat tgagtaaatc tttatttaat
    19981 acttctatta cttctataga tttatcattt ttagtgtata ctttgtggtc attttctttt
    20041 tcaatcttat attccaaatt tctactttca aaatacgaaa taaggtcttt atttgtaaat
    20101 gtatagaaac tactatagag aaatttttta ttactgacaa ctttatcaaa aaaatcttct
    20161 atctctctat tattggtaaa attgcatcta ccgccaccag ttgccctaag ttttcttcct
    20221 aattctccat ttctctcaac taatattact tcattattgt tttttgaagc tgacaaagct
    20281 gccatcattc cagaagctcc tccaccaact actataactt tactcataaa tttctcctta
    20341 taattgattc ctactccagt gcagaattat ttatctttac atgatttttt atatgacttg
    20401 tatcattcat ttttactata acaggtccat aatctttaaa ttctactata tttaaagcag
    20461 tattatcttg cttaattcta tatatatttt ccatacctga ttctaaaaat gacaacaaca
    20521 taaccctcaa tgtaattgaa tgtgtaacaa gaattatatt tttattatca tatttttcat
    20581 ttagttcctt tataaaagca tctactcttt cctttataat cttaagagtt tcgccttctg
    20641 gtatatttac taagtgtggt tcatttctcc atgttgcata tatatctgaa taatcttttt
    20701 gaatttcttt tattaaaagg ccttcccaaa ctccgaatcc catttctcta agtgcctcag
    20761 ttttttgaac ttcaatattg agcttatctc ctaaaatttg agctgtttgc attgctctcc
    20821 caaggtcgct actaaaaata taatcaattg aatacttgcc aatatcttca gaaagttctt
    20881 ttgcctgttc tataccttga ggcgtaaggt cagaattacc atgaccttgt gtttttccca
    20941 atatattcca atccgtttgt ccatgtctta ctatataaaa agtatttccc atatgtcctc
    21001 ctaatatatg cctatttttt attaaatata aattatcact tctcaaaact taattatatc
    21061 atataatact ttactttgtt tctattttat aatctaatct aacatattat ttacaataaa
    21121 tctcatttgt atttctgcaa attattgtac tgttaaagaa tttttctaaa tttataaaca
    21181 tatttctctt atattattta tatacttctt caaatctgta ttcttgaatt gaattgacat
    21241 acttatctgt atctactttt tctaaaaaca tatccaaagg tcttgcaaat attcctgtat
    21301 catcatataa ggtcttgtat aaaactaaat cattagtatc ttcttctttt ttatgataaa
    21361 ggtttccatc taaatcccta taaacataga tatcttcttc tctttcagta tgtctaataa
    21421 ctaatttata tttatttctg ttttggatta aattttcttt accatatatt tcacatatgt
    21481 tttctatatc atcaatagcc ttgctaagac ccattgtagc ataatatttc cctttaaaat
    21541 gtttatagat acaaggatat tttaactttc tttcactgat acctaagtta ttctttaatt
    21601 caatcatttt attataatct cccatatctc gtccctttct tttaaaaagt attttattct
    21661 gaaataagtt acttgtttta tattttatca taatttaaaa tttattaata atacaatcaa
    21721 taaataaaga ggctgtataa aatacagcct cccaatatta atcttcataa tcatataatt
    21781 tttctaattt cttttttaat ttcttttttc tttctgaacc taaaattctt ataaaattat
    21841 tattcacaaa aatacaatta ttgctgagat aaaattcaca cttgtctaga tttacaatat
    21901 aactattact gcatttgaaa aaattttctt ctttcaatag ttttcttata caaggagcat
    21961 tttctccaac ttcataatta ccactttcag tataaatctt tacttcatac tcttgctttt
    22021 ctatgtacaa aacttcttta ctattaattg aaaaacgttt atcctcgtat tcaacattaa
    22081 taaaattttc ctctttcatt tccatccctc caagtacttt tattaattta attttagtgt
    22141 tttttatcaa tataatctat cggaaatata tgtaagactg atttacttaa gatatttaaa
    22201 taattattat taatattaat gtataagttt attttttaac attaattttt aaagttaaaa
    22261 agactttttt taatcatatc cgtatatata agcatatcta tctttaataa aataactttt
    22321 atagaaattc atgtaaaatt atacataaaa aagtaattat taacttttaa tttaaatgaa
    22381 tcgtattaaa atttatctta ttttcctaat tttaatctta agtattatat gtattaatac
    22441 aaagattgaa atgttagata atattaatag taaaactatt actaaaaaag cagttcttct
    22501 ttcaggagag atttttacaa gattaataat ataatcataa agtacaaaaa atgacaataa
    22561 atttgctatc ataggaatac atatgtatat ctctttttta cttattttaa aatttttaaa
    22621 agaagcctta taaacataaa gtaatatacc taacattatc tttcccaaga atattgcttc
    22681 atatctatat aaactgccac taagtaaaac attcatatta tccaaattgc ttactgttgc
    22741 tactaataac atacttaatc catttatacc tattaatatt aaccaataaa aaaatgagat
    22801 taccagacat ttatatatat taaccttata gttagactta tagaaaacga acattacaaa
    22861 tatacatatc aagattctac tattagctcc tatatttata tataaagaag aaataataag
    22921 aaaaacagaa aagagaaaat acatatttag tttttttaag tcggtctttg actgactagt
    22981 ataatcaaaa acttttttac atgaccatat ttctataata gaagaaacaa ttgttataaa
    23041 tgcatctaac ataataatat cctcaaaaat gattaaatta tattttttat aaaatatatc
    23101 ataaaaaata catactaatt atagcataaa aaacacactt aaatttaata taattattta
    23161 atatttttaa caactttcta tagtacattt taaaatattt acagataagt taccaataat
    23221 aaagtttaaa aatataaaaa tcatttctta cttttagttt acctataata acaaaagaca
    23281 ggtttatatt gtaataatta aaaatagtaa aatatatttt gatatggtta aatagaaata
    23341 atactttaga ttaatgaata ttgacaagtt ataaaattat tttaaatttt aaaaatcatt
    23401 tcttatttaa ataagtggta tcatttaact tgatactccc tattccaatt attttaggta
    23461 ttttactaat tagagaacct atttaatatt actttatgaa tagtgagtag ctgattaaat
    23521 aataccattt atggttttca gccatagatt aattaaattc ttagagtttt atcctgatat
    23581 aattactctt cttctaaatt tttaaaatga ctttcaaata aatccaacat atctttatca
    23641 acaacttcat catcatgtag tgctgaaatt aaactctgta aagaattgtc gtgtatattg
    23701 cttaaaaaat cttttgtctc aacacttaaa tactccttat gtccaacaac tattgtataa
    23761 tgagtgtatc tatctatttt ttctgcactt aaaaaatgtt ttttaacaag tcttgataaa
    23821 agtgtcaatg tagttgtttg tttccaacca tattttcgtt ccatagcttc aatagtttct
    23881 tttgatgtta ctgtatcatt tttttcccaa ataaacttca taacttttaa ttctgcttgt
    23941 ggtatttttg aaattgtcat aaccttacgc ccctctcaaa cttttattaa attaatacta
    24001 tcgcaatata gtttttaagt caaagatttt gtcgataata atattttcca atttttacca
    24061 tagagttatt ttttatataa ttctatgata aattacataa aaaatattta ttttacatca
    24121 taaaattaga aatgtaaata gtatgattat ttagtcttat ctttaataaa caatccagta
    24181 aatttataca tccaaagtgc attcataaac aacaataaac ttgttgaaaa aaatactgtt
    24241 ctaataccta aatgtgctga tatttgtccc cctaaaaatg ctcctgatga acaacctata
    24301 tactgggcag atgcattata tccaaatatt tttcccacat attcttctgg tgcaattttt
    24361 ttaagcaagc tgttaacaga aggattaagc cctgcaactg acaaccctaa taaaaatctc
    24421 aacaacatca gttgccatgc agtttttaca aaagcttgtg gtataaatat aatcccagcc
    24481 cataaaagtg aaattaacaa tactttttga ggacctattt tatctgacat ttttccaagt
    24541 ttttgagctg ctaataaatt tgctaatcct gatgcagaaa atgtcaatcc agctattaaa
    24601 gctacatttg atgcaaaatt tgtaagttgt gatatatata tggttacaat tggttctata
    24661 gagtataaag ctagttgagt aatgaatgtg gttacaaaaa tagatgcaat taaaaattta
    24721 tttggcaata tatttaattg ttctttaggt atagaaattg attcttttat ttctttaggt
    24781 ttaaaattat ctactacaaa taaaatagag atgataaatg atacaaacaa caatcctcct
    24841 gttataataa atgtactttt aagacccaag attgattcta tatatcctcc tactgttgga
    24901 ccaattaatg agccagtagt tgatgcagtt gcaagagtac ccaaagccca tcctgaatga
    24961 tttttatctg tctgtgttgc aataagagta gtacatgcag tagcataacc cgtaaaaact
    25021 ccttgaagta ttcttaatcc taataactga taaacatttt gaacaaacgc catggtaaat
    25081 ataactattc ccatcccaat acttgcacgt aaaagcattg attttcttcc atatttatca
    25141 gcagctttcc ctcaaattgg cgaaaagaat gctgctacta caaaggtaca tccaaaaatt
    25201 atacctgaat actgttgtat taaactaaca tcagttaccc ccaactgttt aatatataat
    25261 ggaagtatag gtgctatttg gctcatccct atagaactaa aaaacatacc tatccaacat
    25321 acaaataaat ttcttttcca caattccata tagaattaca ccccctgcat tttaaatttt
    25381 ttcaataata ctaagtttat taatttatcc tattgtttac tcaatatatc catcttttct
    25441 tttataatat tgtttaggaa attcataact cgaaaataat agtggtgata aagaatagga
    25501 gtaacttcca gcattactaa ttgaaatcaa atctcctata tttgcttttt tcaatttaac
    25561 attatttgca attacatcta atgatgtaca gagattccca acaacattta catactcttc
    25621 ttcttttaaa ttatttaata ctgaaagaga aaaggcatga tttgatgtat ataatggctc
    25681 ttgaccttct aattctactc cacaagtaat attttcaatt aaattagaaa taataggtct
    25741 cataaatcca ttaagtccat tttttacaat aagatatttt ttccctcttg atactttaat
    25801 atctacaatt ttaacataat aattacctga tttacaaact atgaaacgtc ctgattcaat
    25861 aagaagtctt gatttcagtg tgtttgaatt agtaaaaaca atcttttgag ttaattttga
    25921 aagctcttta agattaagtg gattttctct aagattatca taagctactc caagtccact
    25981 accaaaattt ataaattcaa tattaatata cttactttta tttaaattta aagctataaa
    26041 aaaacatttt tcataataat gtcctaactt ttcaaaatcc aatacctgag atttaatatg
    26101 tatatgaatc ccaactacag atacataatc acacatactt aacatcttaa aaacactgct
    26161 catatcttct atatctatac caaatttgct tgatttagca ctatcatcat ccatagtaaa
    26221 atcaggattt attcttattc ctattttaac tttctgatta tgctttttag atatcttatt
    26281 tataagttct atttctgtaa tactatctgc aataattatg catttatcta aaacagtttc
    26341 tatatcaatc tctgttttgc ctggtgatga ataataaata tcatatttag aaactccaca
    26401 ttctgatgaa atcataactt cattagttga agaagcatct gctccaaaac cttgctctgc
    26461 aatgcttttt acaattttgt tatatggatt tgtttttata gaatataaaa aatcaaaacc
    26521 taaaatatta tttttcaatt ccttacattt ttcaattata ttagtctcat catatatata
    26581 acatggtgca ttttcttcta taaatttata gtcttcattt gagtacatat tttttacctc
    26641 caagatttta aatataatca taaattaagc aattataatc ctataagata attttacaat
    26701 aaattaccat agttaactac acacatatat gtaaaaacat acctaaaata tttttaatta
    26761 tagatacatt ccaactttta ttgataaaac aatttgtttc aattttatta aaaatataca
    26821 aagtgtattg ttgttaacgt attctcatag tatcctaaaa ctatattaag cactaaaaac
    26881 tattttaaaa tactgtggag gtatcaatat ggaaatatgc attcttatgg gaagtcctaa
    26941 gaaaaaaggt aatactgctg ctattttaaa accttttatt gaggaattag aaaattataa
    27001 ttcaaatatt gaagtaatat ggttatatga ttgtaaaata gagccatgta ttgcttgtaa
    27061 aaaatgtcag gataacttat ttgattttgg atgttattgt aaagatgatg ttcagaaaat
    27121 tttcaagaaa attcttatgt gtgatttaat tgtttttgct actccaattt attcttggta
    27181 ctgcacttct ccaatgaaag cactacttga cagattggtg tatggaatga ataagtacta
    27241 tggtaatgag aaaggccccg ctctatgggc aggaaaacca gtagcactta ttacaacatg
    27301 tggatataga cctgaaaagg gtgctgatat ttgggaaaat ggtatgaagc gctactgtaa
    27361 gcattcacag ttaaaatata taggaatgct tgttgaacgt gatttaggta aacctaattt
    27421 tatgaataaa gaaaaagaaa aacattctcg tcaatttgca aaacaaattt attatatttt
    27481 agataattca attgaagtag ataactgcta aagacataat actccaagta aagtattgat
    27541 aatataaata aatcttacat aatttgctta tttaaaataa gatatatatt cttctaaaaa
    27601 gtaacaataa ttaaaatatt ttttatcatt tcaacaatga taaagaggct tatatctatt
    27661 ttgagacagc ctctttattg ctttttatat gttaattatt ttcatagtaa acatccttaa
    27721 aaactcttta catgttactt ccttcaaaac ctattactca ttcctactat aaatttttta
    27781 taaattcagt tatctcttcc tttgttgcat atgaacttat cgcaccttta atagttgtac
    27841 tacgagctgc ataataatta gaaaattcta aatactcatt taacgtttct tgtttcattt
    27901 cttttatatc attcaatgtt atttgtttat acaataaggt gtataaaaat gaacctatat
    27961 aagaatctcc tgctccggtt gtatctattg catttacttt aaccccttta cacatacctt
    28021 ttactttttt agtgtatgca tctactccat cttttccttt tgtatataat actagttcaa
    28081 cattccccac aaagagttta tgtaatgatt tttctatatc attttctcct gtcataaatt
    28141 ctaattcttc atcagatatc ttaacaatat gagcaaaagg taaaaattct gatattgcct
    28201 ttttacaaga ttgctcacta tcccataaag gtaatcttat attagggtca aaacttatta
    28261 tactattact ttcaagtgcg tattctattg cttttttatg tgctaatttc attggactat
    28321 ctattaaatc tactgaacaa aaatgtaaga tatgacaatt attaaaccac tcttttttaa
    28381 cctcatcagc ttctaaaagc atatctgcac ttggatttct ataaaatgag aaatcacgat
    28441 taccatcttc ttttaatgat acaaaagcaa gtcctgtatt tgctttactt gttctcaata
    28501 cataatcagt atttacattt acttcattta atacatctat tatataatca ccaaatgcat
    28561 cttttcctaa cttagatata aaacttgatt ttcctccaag ctttgctaca acaacactaa
    28621 catttgctgg agctcctcct gctactcttc taaattctga tacatttttt aatttacaac
    28681 cattttgatt aggtataaag tctattaaag cttctcctat actaattact tttttcatct
    28741 tttctacccc ttataatgtg tttttataat tcaatacctt gctttaattt aaatttagta
    28801 tactcatact ctactatttt atcactataa acccttattt gattagaatt ttctttagca
    28861 aatattctac tagtaaatac tacttctcca tcatttataa aaatttctaa aacacttcta
    28921 tccgctagta ctttgatatt agtattttca tcaatattta tatattcttt tctatttgtt
    28981 ccatattcaa caccgaaaac ttttttaaag ttgcttctgt ctaaagttac aatgtttcct
    29041 aatttgttaa attctaattt tacaccttca tcttgtgatg aaaataattc cagcccaaat
    29101 ttattgctgt caatattttt tatattaata ttaagctcat atacattttc atcattttct
    29161 ataatattga tattattctt tatagtattt tgcccacttg tttcatcata tcttaattta
    29221 attaattcat ctacaggcat ttgcaatagc ttgttatttt ttatatttaa ttttcttgga
    29281 acagttagac aatgtgccca tttgttttta tctgtagggt ataaaacttc tcccattcca
    29341 gcccatgcaa atagtattat ttgtgagtcc tttataaatg tttgtggagc atagaaatca
    29401 aatccttttt ccagttcttt cattgtatct atttcaaatg ttaaattatc taaatccata
    29461 ttaccaatag catatactac attatatata ttttgataat caaatccttt aggttctact
    29521 ccttgtggcg aaaatattaa tatatctttt tcatctatat taatataatc tggacattcc
    29581 cacatgtaac caaaatcttc atctatattt tttacattta attctccttt aaaattccaa
    29641 tctatagagt ttttagattc atatactatt ataactcctt ttttatcact tgtttgtgct
    29701 ccaagtaaca taaagtattt atcctttctt ttaaatactt ttgggtctct tacatgtcct
    29761 gtatatcctt ttggtatttc acttactatt ggattatttt catatttagt tattttacca
    29821 tccttgttca ttatagctaa gttttgatat gcatatctat cttctgcact atattttata
    29881 ttccctgtat aatacatatg caattttcca tctacctcta tcgcattacc tgaatatgct
    29941 ccatgagatt cataactttc gattggtatt aatgctacat cttctctatt ccagtttaca
    30001 aaatcatcag aacttacata agcccaatgc ttcattccat gagttgcatc atatggatac
    30061 cattgataaa atacatggta cttaccatta taatatgcta atccatttgg atcattaagt
    30121 agtccatgtt gtggatgtat atgaaatagt ggtttatatt tatcatttaa agatatattt
    30181 cttaatttat ttaattcttc ttttatcgat tctaatatgg ttctatattt aggctctttg
    30241 tacatatttt tattcctttt cttttgtaat tttatatttg ctttattatt actatctaaa
    30301 acaattctta atcttgttac acaatgtgct acacctttta tattttgttt atttcctatg
    30361 tactctaata attactttga tactttgtta caatcagtta tttttgcact cattgtgcta
    30421 ttctttcggt ttaattaaac cggtttcgaa accggtttaa ttaaagatta gcataaattt
    30481 atttcagatg caatatttct ttcaaaaaag ttttcctaat ttttagaatt atagtttttt
    30541 tagtaaaaaa atacacatta ctaatatatg taatgtgtat cttaattttt atttattgta
    30601 ctttcacctt ctattaattc aaatttaatc tcctttaata gtggttcttt tctttcttgt
    30661 attaagttca ctatcgtatt tgctgctact ttacctgcat ttttattttc aaactttatt
    30721 gttgtaagtt tagggcttat aatagacaat acatcatatc ccccaaatcc agctacagat
    30781 atatcttgtg gtatattttt acctctctta ttgatttctt tcattgttgc tattgctata
    30841 ttatcagttg cacatattat tatatctgga ttttcacttt ctagcatttc tccacttctt
    30901 tgtatagacg tttcttggtc aaagtcagta taaaatactt ttgcatcata ccctttatct
    30961 tttaacccat taagtactcc attttttcta tttaaaccaa ctgatatatc actttcatca
    31021 acacctaaat atacaatgtt tttgtaacct ttattagcaa tatattgacc cattttatat
    31081 cctgctccat aatcatcatt cactatgcta ttgataccat caactttttg tccaactaca
    31141 accattggta tatctaactt ttcaatttca ttcttatgtt ctttagttat ttcggtacca
    31201 attagtatta ttccatcaac ttttagacta gctaactttg atatactatc tatttctgac
    31261 cttatttttc tattagtatt aataattagt gatgtatatt ttaattcttt gagttcttca
    31321 tctattgcca tcataatttt agattttaca aaagaatcta aacacggtgt tataatacca
    31381 ataaagtatg tttttttagc tctcaaactt tgagcaaatg cattcggctc atatccatat
    31441 tcatctatta ttgtctttat tttctcttta gtagaatcac tgatatcttt attatttaga
    31501 tatcttgata cagtactttt agatactcct gccaagttag caatatcatt tattgttatt
    31561 tttttcatag gttatcacct tctacatttg atatatttta ttacattata ttatatattt
    31621 aaaataaaat atagttattt gtccagtcaa tactttttat atactacttg tactatccat
    31681 tccacatcca aataattgag tctttaacat taaattaatt gtaattttca ttttttgttg
    31741 ttatatttaa tttcaatttt atctactatg aattgataaa tactctaatt tatttataat
    31801 ataaaaataa ggctatgatt tttacaacca tagccttatc gataaatact taattatcac
    31861 ttaaaaaata attttttatt taaagaatat atataaatat tttaaaattc tataattttt
    31921 aaaactatat aaactttatt ttcatatgtt tttattctcc aattgatata ttatttatgc
    31981 cattattttt aacttcaata attggttgtc catttcctaa aatataatct ttagaccaac
    32041 cttctggcaa tttatttatc ccatttccaa ctaacttcaa gtgtaaattt gatggttctt
    32101 ttttcagatt taatttaaat attccatcaa aggtaccttt attatcgctt ccaaataaac
    32161 ttatattgcc atttatattt gtagctgtta cacttacaat tccattgttt gctctaaatt
    32221 ttatctcaga tgatatagtt tcagctgtta cacctgaaat cccactatta gtatttatat
    32281 tgaaaggatt tgtcacattg ttatcaacaa tgttagcaat tccgtcattg acatcaacag
    32341 tagctcctcc tttaatatta taaagttcta ctgaaccact ttcaacatct actttaaaat
    32401 tagaatacat tttatcggga actccaattt ttatcacatt taccatccta tctttatttg
    32461 catttatcat attgtcgcca ttcatatctt gcaaatcact atctttctta tctatttttt
    32521 tcttagcact tacaactagc tttccatttt ttacattatt agaagaattt tctttatata
    32581 cagaaaaagt acaatttttt tcattagtta cacttacgta atcaaactca aatttattgg
    32641 tattcgtctt ttcaacaacc acacctaaat tatctgattc tataagtatt tcagaaactt
    32701 catttccttc aactgaagtt aagtgtttta aaatatttgg agtcacatcc atattattat
    32761 atttaatcat atctaaacac tgtttatata tattggcatc aatacctcca acttttgtta
    32821 tacttttagc tccttttaaa atacctttat tacttccatc agcaactaaa acgattggtt
    32881 tttctttggc taaaggagat aatgttagtg catctactaa ttgatatcca tttgttatat
    32941 aaaaatctgt cgctccatta taaaactttt ccattacctt tttattggtc tcaaatctat
    33001 catttcctcc gattctagtt gctttagttt catttactaa atcctcattt atcgcagatt
    33061 tgccacctat agcgtaaaca tttaaacctt cagtcttaaa tggaactgat ttcccatctg
    33121 taagtattat agctccttta ttttttgcaa ctacaggtgc tatactcata gcatcagcct
    33181 ctcctacaaa tccatttgta agcattacct cttttacact acttacttca tttatttttt
    33241 tagctacatt ataactagtt tcaattctat tgttaccatt tattctttcc acttgaatac
    33301 cttttctttt tatatctttt tctactgaat tttcaatagt attataacca ccaattatat
    33361 atactttttt tacaacattt aaactcttat tatctagtct atttaaagta gcttttggta
    33421 tttcactttt tttagtcaaa agtattggtg cgttaatagc tccagctaat ccacttgatg
    33481 aaagcccatc agataaattt ttatctccat taactaatat tacagtatca taagtttgtc
    33541 tatctgctat aagtcctgct gtttcatatc tatcaattcc tattattgaa tcttctttaa
    33601 tctgtgcatt tgcagttaaa ttattaaaaa gaaacatagt caaaactaaa a
//
LOCUS       DAEPOZ010000014        33262 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.014, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000014 DAEPOZ010000000
VERSION     DAEPOZ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33262
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.014"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            66..812
                     /locus_tag="KR440_000567"
     CDS             66..812
                     /locus_tag="KR440_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBH0697633.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKLYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            874..1548
                     /locus_tag="KR440_000568"
     CDS             874..1548
                     /locus_tag="KR440_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0697634.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKVEELADWISKYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCLNCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCALRCMMSCPWSL
                     RYANQK"
     gene            1916..3262
                     /locus_tag="KR440_000569"
     CDS             1916..3262
                     /locus_tag="KR440_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBH0697635.1"
                     /translation="MEIETKTPDWINVLELNSKIDITWKYDVSNMIQDIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGDNQVSNLDKKQSIVGTTNFITRGVSNI
                     SIITLEGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLFHFEHLFISGFSGI
                     GIEIPYYSTGNDIIVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     REIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITKCGQYYKSVDYNTYE
                     NMEDRKEEAYSIFYGDILENCNIVLMNSNIDTLENDSLDEHKIYVIKANETNKVMLTC
                     YAEADELIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSIEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            3289..4689
                     /locus_tag="KR440_000570"
     CDS             3289..4689
                     /locus_tag="KR440_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBH0697636.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSSKLQEIIDAAPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENIAI
                     ITAKSVRHCIKSICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAIVCEDKQ
                     GEPGHYENLFFSGFSGIALDMPNNSTANDITVFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNKDNWEDFKEDKH
                     KVYLVEALETKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSKDKVIKMHKPEVGDIMSTIIKDENILSAHYGGTWGKIGE
                     KIEFGITVHYYKLIEK"
     gene            4899..6335
                     /locus_tag="KR440_000571"
     CDS             4899..6335
                     /locus_tag="KR440_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBH0697637.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNIGPDLQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSPTETTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNEEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKDYEGYGSYKINNITIDQCGYCGFLFDSVKGSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSSPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIETSYGGKW
                     EKLGSEVIGKETVYYYKHHEPDKTNQIK"
     gene            6679..7623
                     /locus_tag="KR440_000572"
     CDS             6679..7623
                     /locus_tag="KR440_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HBH0697638.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLKEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLKDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGIILEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            7792..8724
                     /locus_tag="KR440_000573"
     CDS             7792..8724
                     /locus_tag="KR440_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBH0697639.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            8829..9296
                     /locus_tag="KR440_000574"
     CDS             8829..9296
                     /locus_tag="KR440_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HBH0697640.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGETMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(9540..10394)
                     /locus_tag="KR440_000575"
     CDS             complement(9540..10394)
                     /locus_tag="KR440_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBH0697641.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            10560..12524
                     /locus_tag="KR440_000576"
     CDS             10560..12524
                     /locus_tag="KR440_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH0697642.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLSEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYIGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVAADQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSCCTSKK
                     YKSFRNLIFEENILLNIEASNYEDAIKYITDNLIKKGFVKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLLEESI"
     gene            12524..12985
                     /locus_tag="KR440_000577"
     CDS             12524..12985
                     /locus_tag="KR440_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0697643.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSDAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            12988..13308
                     /locus_tag="KR440_000578"
     CDS             12988..13308
                     /locus_tag="KR440_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBH0697644.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            13324..14424
                     /locus_tag="KR440_000579"
     CDS             13324..14424
                     /locus_tag="KR440_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBH0697645.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            14544..17144
                     /locus_tag="KR440_000580"
     CDS             14544..17144
                     /locus_tag="KR440_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBH0697646.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKHVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDYILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNVFIEESGYYILKVR
                     INVQLPALGYKVVSFESSDKEMVSLDKSNDTFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLILKDSGNAGDTYDFSPLKNDEDIKLSFDRVYTEKTLGYEKLVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRENGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTVFTRGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKKLEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPEKY
                     LISACYPSYYSKDKFIIRVENPSSNRMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
     gene            17613..17948
                     /locus_tag="KR440_000581"
     CDS             17613..17948
                     /locus_tag="KR440_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0697647.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            18085..19362
                     /locus_tag="KR440_000582"
     CDS             18085..19362
                     /locus_tag="KR440_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HBH0697648.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNNSQKDSNNDNNDNG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKDQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            19483..20871
                     /locus_tag="KR440_000583"
     CDS             19483..20871
                     /locus_tag="KR440_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBH0697649.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRVLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSESAIVEYEVDGKSKELTSNMILVATGRKANTEGL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
     gene            21242..21955
                     /locus_tag="KR440_000584"
     CDS             21242..21955
                     /locus_tag="KR440_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017208807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HBH0697650.1"
                     /translation="MNKISKRGFDDSDKRWFSSKELTRLIKAKEEIEWLLNRDYKIDP
                     VVTFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLFLDKIKEDTVNIDGFNLIISI
                     EVALSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDA
                     PVSNSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKECNIINLNK"
     gene            22073..22942
                     /locus_tag="KR440_000585"
     CDS             22073..22942
                     /locus_tag="KR440_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBH0697651.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANVNLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLREDKSITSNIITV
                     IPEKSRVEVLQVDGDWSKIVYDDKIGYVFNYFLSTDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(23097..24242)
                     /locus_tag="KR440_000586"
     CDS             complement(23097..24242)
                     /locus_tag="KR440_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBH0697652.1"
                     /translation="MSTYYVPPINLLGKGCLNEAKDSIKSLGTKKAFVVSDKFLVSNG
                     TVKKVTDILSQIDVDFVVYDEVKQNPTVTNVDKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIVAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAVEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKEKIIELFMKIS"
     gene            complement(24575..25351)
                     /locus_tag="KR440_000587"
     CDS             complement(24575..25351)
                     /locus_tag="KR440_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBH0697653.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
     gene            25933..26907
                     /locus_tag="KR440_000588"
     CDS             25933..26907
                     /locus_tag="KR440_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBH0697654.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
     gene            27005..27310
                     /locus_tag="KR440_000589"
     CDS             27005..27310
                     /locus_tag="KR440_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBH0697655.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGNEISFKLKDKTVKTITALDNI
                     TIYHKLAIKDMAQGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            27466..28626
                     /locus_tag="KR440_000590"
     CDS             27466..28626
                     /locus_tag="KR440_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBH0697656.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            28676..29578
                     /locus_tag="KR440_000591"
     CDS             28676..29578
                     /locus_tag="KR440_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBH0697657.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESIFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            29658..30551
                     /locus_tag="KR440_000592"
     CDS             29658..30551
                     /locus_tag="KR440_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HBH0697658.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNKLNIC"
     regulatory      30604..30785
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            31013..32143
                     /locus_tag="KR440_000593"
     CDS             31013..32143
                     /locus_tag="KR440_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0697659.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTEEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     gene            32215..33060
                     /locus_tag="KR440_000594"
     CDS             32215..33060
                     /locus_tag="KR440_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBH0697660.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KLIKK"
BASE COUNT        13045 a         3545 c         5794 g        10878 t
ORIGIN      
        1 tatattaata aactttaata taatatattt atgaaaaatg taaattgtga aatggaggta
       61 gtaacatgaa aattgaaaat ctttctattg atgatattaa acgaggctat atatttaatg
      121 atgatttaaa ttgttatagt tgcattaaat gtacaaaaac atatgaggta ggagaaatat
      181 ataaaatagg taatagattc tttgaagctt caaaagctat tgaacttcat atggaattag
      241 aacatagtga ttatttagag caattgctta atagtgattc aaagtacaac acattgactg
      301 aaaatcaaaa acaattattt caattattta ttagtgattt accagataag gagatagcaa
      361 aacaggttgg agtatccaca tctactatac gccatcaaaa atttattttc agagaaaaag
      421 caaaacaggc taagctctat ttagcactat atgaatctgt atttgaagat aaatctaata
      481 aaagtagtac tataatacca atccatgaac atgctcgaat ggtagatgaa agatatgtag
      541 taactgaaga agagagaaaa cacattttgg atacatcatt tgaaagtatg aatcctttaa
      601 aattaaaagt attttcttca aaagaaaaga aaaaggtcgt aattttatct aagattttgg
      661 aacaatttga acgtgggaag aaatattcag aaaaggaagt taatcagatt ttagaatcta
      721 tatatgatga ttttgcaaca attagaagat atttaattga gtatggcttt atgagtagaa
      781 ataaagaatg tacaaagtat tggttaacat aattaaaata ataaatttta ctaatgtgta
      841 atgttatttt tatctagggg ggaggagtta aatatgaatg atgaattaaa atctatttta
      901 tacaataaag gtgtagacat tgttcgcttt gttgatatat ctgaatttcc aataaatcaa
      961 acacaaggtt tttctaaagc aattttattt tgtatagggt tatcaaaaaa atttattaca
     1021 gatatataca ataacttacc tacagatagt gatgagtttt tggaaaaaga ggagaaagta
     1081 gaagaattag ctgactggat ttcaaaatat attcaaaata aaggatatag agcgtattct
     1141 caatcggaga aaaacaatct agaacatgga tattttgaaa aagcctatat taatccagaa
     1201 atgcaatcag ggattagtcc tttaccacat aagacaatag caaatattag tggcattgga
     1261 tttatgggaa aaaataattt gtttgtaaca gaggaatacg gatgtgcttt tagcatgtgt
     1321 acagtactta cagatgctcc aatttctgtt gaaagatatc ctttgattga ttcaaaatgt
     1381 ttaaattgta atgtttgtgt agagaactgt cctgcaaaag ctatacatgg aaatgaatgg
     1441 actttacctg gaaaaagaga atctattatt gatgtttcaa agtgtttttg tgcattgagg
     1501 tgtatgatga gttgcccatg gtcattaaga tatgcaaatc aaaaataact ttactagaga
     1561 aggtattaaa ttgaatatta taaaattttt aaaatgtaag attatataag atttctcaaa
     1621 ataaaaattt gtatagaaca ctcagaaatg agtgtttttc tattgggatt aaattatatt
     1681 gatacaaagc ttaactattt taatcattat ataaaatttg tattaaaaaa ttaagaataa
     1741 cttgctaata gtaaaaatat aatgagatta taattgattt ataatataac tgtaatgatg
     1801 ttgtaaaatg aaaattgtca tctcaaaatt cccaaaaaga gtaaaagtca cttcggttat
     1861 agtcaaaaaa ttttttctgt gaaaagaatt tagatttaaa agggaggaat taatcatgga
     1921 aattgaaact aaaactccag attggattaa tgtactagaa ttaaacagta aaatagatat
     1981 tacttggaaa tatgatgtaa gtaatatgat acaagatatt gtctctgata agtcactaga
     2041 aggttccatt atatattttc caaaaggtaa ttacttattt gagaaaggta ttaaaattag
     2101 tcaacaaata acacttcaag gagattctta ttatggagga gataatcaag tatcaaattt
     2161 agataaaaaa caatcaatcg taggaacaac caattttata acccgaggag tttctaatat
     2221 aagtattatt acattggaag gaacaagtca atgtatcaaa aatattaatt tttattatga
     2281 tagtcatgat atagagaaaa taccacctaa aaatgtatct gctattacag aatatggaga
     2341 aacacaagga ctatttcact ttgaacacct gtttatttct ggattttcag gaatagggat
     2401 tgaaattcca tactattcaa ctggcaatga tatcattgtt agttcatgtg gtcttggtat
     2461 gaggcttgga gagaagtcaa tgctttctag tagcaagatt tatgaatgta aaaatggaat
     2521 ggagattact actggtgtta gcttaaataa tgtgagaata gaagaaataa gagaaatagg
     2581 tattaataat aaaggatttg gttttaacct gattatgaat cttatggtcg accaatgtgg
     2641 atattgtgga tttatgtttg aaaaaatgtg ttatagccag ataactgcca gaattactaa
     2701 atgcggtcag tattataaaa gtgttgatta taatacttat gagaatatgg aagatagaaa
     2761 agaagaagct tattccattt tttatggaga tattttagaa aattgcaata ttgtattgat
     2821 gaatagtaat attgatacct tagaaaatga ttcacttgat gaacataaga tatatgtaat
     2881 caaggcaaat gaaacaaaca aagtaatgtt aacatgttat gctgaagcag atgagcttat
     2941 acagtgcgca aagggcaact tgttgctaga aaatggtgaa aatacttata aattttataa
     3001 tggtgagatt tgttctgtag gaggtgttca aatttctgat atagatgaaa aagatttaat
     3061 caaaataatc gagaacactc tgtatataga tgataaaggt aagtacttaa tggttcctag
     3121 attaaaaaag aatactgtaa tatattcagt actagattct atagaagaaa taaataaatt
     3181 acatggtgga atatgggaac agattgatgt aaaagtatta gctggggaat ctatgtacta
     3241 ttataaaaag ataaaagatt aaattatcaa actaggaggt agtgattaat ggaagagtct
     3301 ataataatta aagatataag tggctggatt aatgtacttg attatgggat ttgtaatgat
     3361 gaaacaaaag atgtaagttc caagttgcaa gagattatag atgctgcacc agaagattct
     3421 attatatatt ttccaaaagg aaaatactta tttgaaagtg gtgttaagat taagaaacga
     3481 ttaaccttat gtggagattc ttatattttg gcgaatgttc ctaaaaacac tgctggagta
     3541 acacaattta cttttaaagg aaagaaaaat cctaaaagtg aaaatatagc aattataaca
     3601 gcaaaaagtg taagacactg tattaagagc atttgctttt attcagatag ttgtgataga
     3661 gatatgacat ctaatcatga acctccaaca gatggaaaac ctagatttca tcataatgta
     3721 attataaatt atagaaatat atcggctatt gtatgtgaag ataaacaggg agaaccaggt
     3781 cattatgaaa acttgttttt tagtggtttt tctggaattg cacttgatat gcctaataat
     3841 tctacagcaa atgatattac agtttttaca tccggacttg gaattagtac aggggaaaat
     3901 tcaacgattg ctaacagtaa ggtttggggc tgtgttgatg gaatgataat ttctactgga
     3961 acttttataa atggtatgag ggtagaagaa ataaaaaaag ttggaattaa aaatattggt
     4021 agaggtttaa attttattac aaatatcaca attgaccaat gtggatattg tggagtttgg
     4081 tttgattcta tatccaatgt tcagatatca ggaaatatca caaggtgcgg acagtactat
     4141 tatgatgtaa gctatgataa ttatttaatg ttggataata gagttaaaga agcatattct
     4201 ttattctatg gtaatttatt ggagtcaagt aatatagttt tgtcaaatac caataaagat
     4261 aattgggaag atttcaaaga ggataagcat aaggtgtatc ttgtagaagc acttgaaacc
     4321 aagaatgtac tattaacatg caatatagat gctactgact ggattgttaa gtcagaaaaa
     4381 ggaaatttaa cgtacaataa tatgcgacaa actgttatat tttacgatgg aaaactaagt
     4441 agtatcaatg gaattggtat atctgatgaa gattatgggg ataaagtcaa gataaaaaaa
     4501 ggaaatatat atataaatag taaggataag gttattaaga tgcataaacc agaggtaggt
     4561 gacattatgt caacaattat taaagatgaa aatatacttt ccgctcatta tgggggaaca
     4621 tgggggaaaa ttggtgaaaa aatagagttt ggaataactg tacactatta taaattgata
     4681 gagaaataag taaatacgta gtatttgaat agatgtattt tattggagag ctaggactat
     4741 agttttaatg taaatggttt ggtaatagca tggattgaat aatataaaaa taatataaaa
     4801 aattaatttg attatgtaaa attctatgaa gtagtaaacc tgtgacttta gtcatggtag
     4861 gttcaaatta agtaaatggg aaaggaatat ataatattat ggaaggaaga actgggatta
     4921 aagatggaag atggattaat gtagtagaaa gaggtattaa aaatgatggt tcgtacaaca
     4981 taggtcctga tttacaggat attattgatg aaatagaaac agatttctgt gtcttatatt
     5041 ttccaaaagg aaaatactta tttacaagtg gtgttaaaat taataaagaa attgtattac
     5101 aaggtgattc taattcacct acagagacta cacaatttat aactcgtgga gtagaaaata
     5161 tgagtattat tacattgaca gggaagaaac aatgtattaa aaatataaat ttctattctg
     5221 atagttgtga gatacaggta aatgaagaac caccgacaaa aggtaatcct aaatatcatt
     5281 atgagatgat tattaataag aatgaaaaaa atgttgagat gaataatgta tcagcaattc
     5341 tgtatgataa tagtaagaat atgattaaag gtctaggtca ttatgaaaat atttatataa
     5401 gtggtttttc gggtacagga attaggatac catattactc aattgtaaat gatattacag
     5461 tttcatcttg tgggttgggt attgatacag gaatagacac aattatttct aatagtagaa
     5521 tatctaagtg tcaaaatgga atgaggatta gaactggaac tagcataaat aatgtaagaa
     5581 ttgaaaaaat acagaaagtt ggtcttataa gtagtcaagt taaggattat gagggatatg
     5641 gaagttataa aattaataat attacgattg accaatgtgg gtattgtggt tttttatttg
     5701 actcagtgaa aggtagttat ttttctggta ttataagaag atgtgggcag tattattata
     5761 atacagatta tgacacatat ttaagtatta aagatagagt agaagaagca tattctttat
     5821 tttatggaaa ttattttaca gaatgtaata ttgatttagt tagtgataat aaggataact
     5881 gggatgatgg cttagaatat aaacataaag tttatgtatt taaaaccctt caaataccaa
     5941 aattaacatt gagatgcaat atagatgcct atgattatat cgttaaatcg gtacatggag
     6001 gaggtaattt aatgttaaaa aataacttta acaattatat atttattgat tcatctagtc
     6061 cagaagttat taatggaatt aattttaatt atcaaaagaa tggaaacctg ttaaggttaa
     6121 ataatggtaa attaagttat aaaaatggaa aggaagaaac agctatattc agacctaatt
     6181 atggtcttat tgtagcatca acagattcag aatgtgaaca aatagagact agctatggag
     6241 gaaaatggga gaagcttggt tctgaagtaa ttggaaaaga aacggtatat tattataaac
     6301 atcatgaacc agataaaaca aaccaaataa aataaaattt gtattatata atgatataaa
     6361 gttttaatat atagtgttta cctttaatgt aatattggta tatttgtata gtattaaatt
     6421 tgttaaaaag taaaattgta gggtgcttga aaatagcacc ttttttagtt tcattataaa
     6481 attagttttg aaagtttagg aataatgtat atttattttt aaagaattat aaccttatta
     6541 aggatatttt aaatgctaga aatgaatcag taaatcatac tggaattatc ttaaatgttt
     6601 tgattaaatt aagtatttag agctataatt atattaaaaa tatattagga cataattata
     6661 ttactatgga ggttaaatat gatagtaagt gtgagtagaa gaactgatat acctgctttc
     6721 tatagtaagt ggttttttaa cagacttaaa gaaggttttg tttatgtaat taatccaatg
     6781 aatccaaaac aggttagtaa gatagagtta aatccacata ctgttgattg ttttgtattt
     6841 tggacaaagg atgtaactcc aatgttatat gatttaaata aattaaaaga ctataaatat
     6901 tattttcact atactataac atcatatgga aaagaagtag agacatgtat tttagataaa
     6961 agaaaggtaa tagattcatt taaggaattg tctagaaaaa taggcaaaga aagagtaatt
     7021 ttaagatacg acccgatttt cttaagtgaa aagtacacag tagattatca tatcaaagca
     7081 tttgcgagtt tatgtaatca gcttgatgga tttacagaaa aatgtataat aagttttatc
     7141 gatttatata agaaaacaaa atttaatact aaagcattac atataaagcc aatagaaatt
     7201 acagaaatag aaacattatc aaaagaaatt tctaagattg ctcataagca tgggataata
     7261 cttgaaatgt gttctgaaga atataattta agtaaatttg ggattggaaa aagtaagtgt
     7321 attgatgata gattaatatc aaaaattata ggatgtgaag tagaggtaaa aaaagatagt
     7381 acacaaagag atatttgtgg atgtgttaaa agtgtcgata taggacaata caatacttgc
     7441 agacattatt gtctgtattg ttatgctaac tttaattatg caaaagtaga agaaaactgt
     7501 aggctttata aagaagataa taaattactc atagatactt taagagatga tgcaaaaata
     7561 agcattagag atatgaagac tattaaagta gataagaata aacaaattag tatatttgat
     7621 taaaataaat atactaatta ccttaatata tcaagaatat agatactttt gataacaaaa
     7681 ttaaacttaa tagatatttt ctatataaga aagatggtca taatatgtca atttgaccat
     7741 ctttttgata taattaaaac acaaataatt tagtaacaaa ggaggcagaa cttgaattta
     7801 ttaaaaaaat atcctatacc tattgcaagt ttgattttgt caatttttac tttaggtaac
     7861 ttattacaat cttatagtga atcattacgt aatgtaattg gatttatagg atttattttg
     7921 tatgcaattt atatagtcaa aataatatta cttagaaagg gtgttaaaga gcaattagag
     7981 aaccctttaa tatcaagtac atttccaact ataacaatgg caacaatgat atttgctaca
     8041 tatataaaac cattatcttt agaattaagt attatgattt ggagcatagg aattgtgggt
     8101 catcttgtgt taatagtttt atttagtaag aagttcttag ttaaattctc aataaaaacg
     8161 gtatttccat cttggtatat agtatatgtt gggatagttg cagctagtgt tacggcacca
     8221 gcatttaatc aacttttgat tggcaaaatt gctttttgga taggatttgt atcttatata
     8281 gttcttatac cagtaatttt aaagagagta tggataatca aggaaatgcc tgaaccatct
     8341 ttaccaagta tagctatatt agctgcacca gggtcactat tattgtctgg atatattaat
     8401 tctttttcag ataaaaataa tgtagtatta tacttgttat tcatattatc aattgtattt
     8461 tatattatag ttttgggata cttaccgaaa ttattaagat tgccttttta tccgagtttt
     8521 gctgcattta cattccctat ggcaataagt gcaatgggta tgaaactaat gaatggttat
     8581 ttaactaaat caggtcattt tataagttgg atttcatatg cttctaagtt agaagagata
     8641 attgctacag tgattatttt atatgtttta gttttattta ttatatatct tagtaaatct
     8701 gaaactacat ctaaagctaa ataagaatta ttaaatatgt gtatttaata ggaatattga
     8761 tataaatata tgaagataag ataaaccata gaaacttaat ttagcttaat tttattggag
     8821 gaaaacaaat gaaaacaaaa gaagaatatt taaaagaagt tagaaacata tcagaagaat
     8881 actttagaaa aggagagttt ttttgctctg aagcagttct tcagacaata aatgatgctt
     8941 taggccaacc tttaagtcca gagataacta aattagcatc aggatttccg ataggacttg
     9001 gaaaagctca atgcctttgt ggagcgatat ctggaggaga gatggcttta ggtatagtat
     9061 atggaagagt acatggagaa actatgaatc caaagatgtt tgagcatgct aaaggtttgc
     9121 atgattatat taaaaaagag tatggtgcaa catgttgtag agctattaca aagaaatggg
     9181 atggtgataa ctttatgtct cccgaaagaa aagagcactg tattaaaata acaggtcaag
     9241 ttacagagtg ggttgcagat aagcttatag aagatgaaaa acttaatatc aaatagtgta
     9301 atattagttg gtgaatttat aaagttaaat ttacttatag tataagatac ttgatattct
     9361 cgtattgata gaaaaattta tagcatatgt atttaatgaa atatacttat atttagagtg
     9421 tggtttaatt tcataaatac atatgctatt attttaagta tataagcaaa tttatttaaa
     9481 taaatagaaa cagttaattt atatgtacaa aactatatat gattagtgta aataaatacc
     9541 tattttataa tactcaacat aaagacacca gacatgatta gtattatacc aataaaaata
     9601 gcaagccatt cttttttgtt cttcttttct ttaaaaaata taatccctcc taaagtggct
     9661 actatcacac aactctgaga gatagaaaaa cttattgtaa ctcccaatgt attagtcgtt
     9721 atgaacatac ctaaatttgc tattgaccac aatataccag taattaaatt gaataaaaca
     9781 ttttccctat atacaatctc tttagaaaaa tacttgacaa taatagatga agttatcaac
     9841 ataccaacag attgaggtaa tataattcta tatccttcaa ttaaaaatat ttgattagtt
     9901 acaacatata atgataaaaa tagagatgat aataatacaa ctccaacaca ttttaacata
     9961 tttttatctg tagatttttt agtttgataa ttagtgagaa gtataccaag tattatacat
    10021 aatattgata aagaaccaat cagtaacatt ttagaactgg tccattcatt aaacaagatt
    10081 acagctaata atgtcgtaaa agttaattgt gaaccatttg aaattggcat tactttagat
    10141 acactagcaa tttgaattgc tttaaactgc attaattgtc ccaaactcca gaatatgcca
    10201 gaaatcatac ttatacaaaa tgaaaaaaat gtaatttcag gatgtataac cagagttatt
    10261 atagtggaaa atataaacgc acttatggat gtacctaaaa gctgttcatg aggagaactt
    10321 ttctttagat ttgcaactat gggcatcaat ccccatccca ttacaggaag taaagcaata
    10381 attattgtca gcattaaact atttcctttc atattaaatt tagacagctt ttaatttatc
    10441 atctaaaatt aaagtgttca atacataaaa attaccgtat ttaatcagga aatattagta
    10501 gttttttaga aaaacatttt ccattaaaat aaagataaag ggaaggagta aaattgaaaa
    10561 tgttaactga tatgattcaa aatcgtgaaa ttaaaataat aaagttttta ttaaaagaag
    10621 gtcatacaac tgtaaaagca atagcagatg atttagatgt ttcagaaaaa acagtttcaa
    10681 aatctcttaa agaagtagaa gcagtgctta aaaagatagg tctttcttta ataagaaaac
    10741 ctaagatagg tgtttatatt gatggagatt taaaaaaaat acttccttac ctagataata
    10801 aaggaagtca aattcctaca accagagagg agagagttat ctatatatat tcagtacttt
    10861 taaagagtaa tgattatatt actatacaac aactttctga tgaactatat ataagtcgtg
    10921 gaactattga aagggattta tcagaagttg aaaaactgct aaaaaacgaa gggatttcca
    10981 tatataagaa acctagtaag ggaataaagt taaatataag tgaaagagat aaaagacaac
    11041 ttactgctaa gtttatacat aaattttgga gtagaaactg gtatattaag caagagcaag
    11101 aaagctttta tcaagcattt gaaaagatac aggcagatgt aaatggtatt ttttcggaag
    11161 atgatttatc aataattatt gatattctta ggaatttcag cagagaaaga aaatttgagt
    11221 tctctgatta tgaatttcag tcattagtta ttcatttggc aatagctgta gagagaatta
    11281 gaaaaggcga atatatagaa gaaagtttgt atattggtaa tatagaaaat aatcaaatgt
    11341 caaataccaa ttatttagca aaacaattag aagaaaaatt ttcaatatta ttaccaaaat
    11401 ctgaaattgg atatataaat ttacatttag tagcggctga ccaacataga gatactaatg
    11461 aactaaaaaa tacttcaact aatccttgtg aaacaagtac aaataatata tcaaatcttc
    11521 gggaaattat aaaaaataca ttgtatgaga cagcttatga caaggattta attgatggat
    11581 tgacttcaca tgtacattca tcaataaata gactgaaata tggacttagt attaaaaacc
    11641 catatgtaaa tacaatcaaa caaaactttt ctttatcatt tgagcaagct ttagatttaa
    11701 agaaagtaat tgaatttata tatgatatta gaataaatga tgatgaatgt gcttatatag
    11761 cattacattt tgaagctttt agagaaagaa tgaaagaact tccacaaaat attagagtaa
    11821 ttctagtatg cagtacagga ttaggttcat ctaggttatt gtctgcaaga ataaaaaaat
    11881 actttccaat gataaagatt gaaaaagtac tttccgtaca agaattagta tccagtaaaa
    11941 ttgatgcaga tttagtaata agtactattt atttagagtt agaaacaatt ccaaccatag
    12001 tagttagccc tattttaaat gaaagtgata taagaacctt ggaaagaaat atacaaaatg
    12061 tttcatgttg tacttctaag aagtataaat ctttcagaaa cctaatcttt gaagaaaata
    12121 tattgttaaa tattgaagct tcaaactatg aagatgcaat taagtatata acagataacc
    12181 taataaaaaa aggttttgta aaagaaggta tagctaaaag tgcaattgag agagaaaatt
    12241 tatcttacac gtcttttcag tcaatagcta cacctcatgc taatcctagt tatattaaaa
    12301 aatctaatat atctgttgca acgttaaaaa atcctattac atggggaaat gaaaaggtgg
    12361 aagttatttt ctttatttca ttagaaaatg acaaaaacct agagcttgat gaaatatatg
    12421 aagtttttta tgactttatt gacaataaaa aaaatataaa tgacatatta agtgcaactt
    12481 ccacaaagga agtgtacgat ttgctattgg aggagagtat atagtgaaaa tatcagatgt
    12541 attgaaaaaa gagcaaattg tttttaatct tgatacaaat acaaaggaag aaacaataaa
    12601 aaaattaagt agtatatttg ttgaaaatgg gattgtggac aatgaagaag aatatgttaa
    12661 gtctgttttg gaaagagaag aacattcaac tacaggtatt ggtaatggta ttgctattcc
    12721 tcatggaaaa agtgatgctg tcaaaaaatc ttctataata tttgcaaaga ctaagcataa
    12781 gattgagtgg gatgctttag atgaaaaacc agtagatagt attttcttat tggctataag
    12841 caatattgat aaggatagca atcatcttgt actattatct aaattagcaa ctaaacttat
    12901 ggatgatgac aatgtagatg cactaaaatt agccaattca gaacaagaaa taatcaatat
    12961 atttagtgaa gaaggagaga tgtagaaatg aaaagaaaaa ttatagcagt tacagcatgt
    13021 gcaacaggag ttgcacatac atatatggca gctcaagcgt taaagaaagc ttcaaaaaag
    13081 atgggtcacc ttataaaagt tgaaacacaa ggagctacag gaatcgataa tgagttaact
    13141 acaaaggatg cacaaattgc agaagtagta atatttgcag tagatactaa agtcagaaat
    13201 gctgaacgtt ttgagggaaa agaaatacta gaagtaccag ttaatgcacc tataaaggat
    13261 gcagagaaag taataaatga tgcattaaag ttaattgaca ctaaataaat ggagggaaat
    13321 gtaatgttga aaaagatagt ctctgagtta aaaaagcatg tcttaactgg aatctcatat
    13381 atgattccat tagtaattgc aggtgctatg ataatggcaa tatctcgtgt aggtggttct
    13441 ttctataata taccagatat atgggatgct aaatacgcag aaagtgctag tagtattgtt
    13501 agattattac atgatctaga tggatttgga ggtactgcat taggattaat gtttcctgta
    13561 attgctgcat ttataggatt ttctatagct gataaattag ctattgtacc aggtcttgtt
    13621 ggaggtatga ttgctaaaga tataggtgca ggatttttag gagctttggc agttggtctt
    13681 atagcaggat atacatgttt gtttattaaa aatcatgtta aattaccaaa atcagcagct
    13741 tcaatagtac caatatttat agtaccagta tttggaacat taatcactgt tgtattaatc
    13801 aattatgtga taggtatacc ttttgccaca ttaaatactg gattagaaaa ttggttaaat
    13861 ggattatctg gtacaaatca gatattaatg gcagctatta taggggctat gatggcagtt
    13921 gacttaggtg gaccagttaa taaagcagct ttaacaacat cacttgcatt attaacaagt
    13981 ggaatttatg cacctaatac tgctgcaatg gtaggaatag ttataccacc actaggattg
    14041 ggacttgcta ctatactagc aaaacaaaaa tataataaac agttacgtga agctgggaaa
    14101 tcatctctta taatgggttt aattggagta agtgaaggag caattccttt tgcggtagaa
    14161 tctccattaa aagtaatacc ttcatgtgta attggaacag caattgcttc agctatggca
    14221 gttggtttag gttcagtaaa tgcaactcca attagtggat tttatggatg gtttacagta
    14281 gagaattggc caatgtatgt gttatcaata gcagttggaa cagtaattgt agctggatta
    14341 gttatagtat tgcgtggaaa tccagtgatt gaggatgatg aatttgaaga tgatgatttt
    14401 gatgaagcag agtgggaatc ttaaaattta ataactattt aagacgtgtt ataagctata
    14461 ttcaatatga atcttatact aaataaagtg ttaaatattc agtttagact taaatataaa
    14521 agataaagaa cagaggagaa taaatgataa aagcacatat tgttaatcat acacactggg
    14581 atagagaatg gtattttact tctggagatg cattagtact tagtgaacaa ctatttacag
    14641 atgtaattga tgaattagaa cgtaatccag atgtaagttt tgtacttgat ggacaacttt
    14701 ctattttgga tgattatgta caattacaca aagaaaaaat agaaatcatt aaaaagttaa
    14761 taaaagaaga tagactacat attggaccat ggtttactca aactgatgca ttctttgcta
    14821 gaggagagtc tattttaaga aacttaatga ttggaatttt tgaaagtaaa aagtatggta
    14881 agtatatgaa aattgggtat ttgccagata catttggatt taatgcacag atgcctatac
    14941 tattaaagca tgtaggtctt gataatatat tcttttggag aggtatacat cttggtaaac
    15001 atgttcaatc accatatttt aagtggaaaa gcttaaatgg agaaagttat atatatgcag
    15061 taaatatgcc acatggatat ggaactggta tgcttttaga gccttcatta aagtatgtta
    15121 atggacgctt agacccagct attgatttta ttaaatcata tactgatgta gatgaggtgt
    15181 taataccttc tgggaatgac cagttaaata taataggtga tttcaaaaat aaaattaaag
    15241 cgattaatga tatcggaaag tattcatata taacgagttc atatcaagag tttttaaaat
    15301 atataaagtc catagaatca ctagaaagct atagaggtga atttagagaa cctgtattag
    15361 caagaataca taaatcaatt ggttctagta gaatggatat aaaattagca tgtgataaac
    15421 tagaaaacaa gctgattaaa agaatagagc cattattagt aattgctaaa aagagtaaaa
    15481 ttgaaataag caatcagtta cttataaata catggaaaaa acttttggaa ggacaagcac
    15541 acgatagtct agctggatgt gtaactgata ctgtaacaga ttatattctt caccgtataa
    15601 gagaagcaaa tgaaatatgt gacagtattg aaaatactat tgtaaaaaag atttcagaag
    15661 ggcttaaatt atctaaaaat gatattttag tatttaatac agaagctaag cgttttaatg
    15721 gatataaaga gatacaagtt gtaagtgata gtaaaaatat ttattttaaa gataatttag
    15781 atgctactat aattgaagaa acttatgtaa aaccgagaga aaatgtatta gaagaaacac
    15841 ctgctggcaa cgtatttatt gaagaatcag gatattatat attaaaagta agaataaatg
    15901 tacagcttcc tgcacttggt tataaagttg ttagttttga atcaagtgat aaagaaatgg
    15961 tatcacttga taaatctaat gacactttta tatctaatga taattataag ataatctatg
    16021 aagatggaag cttaaattta caacttaaag atggaactta catatcaaac tttttaatat
    16081 taaaagatag tggtaatgca ggcgatacct atgacttttc tccattaaaa aatgacgagg
    16141 atataaaact ttcatttgat agagtgtata cagaaaaaac tttaggatat gaaaagttgg
    16201 tggttaaagg tactacttta ctaccactta cattagaaga tagaaaaaac aaatatctta
    16261 atgggaaatt aaatgtaaaa gtaagtattg tcttatccaa agaaaatcca ttgatggatg
    16321 taaaactttg tgttgataac acgatatata atcatagact acgtgttcat ataaaaactg
    16381 atataaaaga taataaaaac atagcatcat tgccatttgg atatataaca agagaaaatg
    16441 gagttttaga caactgggaa gatatttaca gtgaaatgcc tatagattta gaacctttgg
    16501 aaagtaatat tactctaaca aaccaaaata gaagctgtac tgtatttact agaggaataa
    16561 aagagtatca acatattgaa gatgaaattg cattgacctt acttgctact acagatgaac
    16621 taggaaagcc tgatttatta tatagaccag gacgtgcttc tggtgataca actaaaaaag
    16681 gtcatataag aattaaaaca gataaagctc aaatgcttaa gaaattagaa ttttcatttg
    16741 caatatatat ggattctaaa tcatttgatg aattggaaat tgcgaattta acccacaatt
    16801 atctaaggga atgtgtaaat tatcaattac aggattacaa tttcttttta tatcgtatag
    16861 ataataagat tcaaaaatca attgtcaata aaactgtaag tagagaatta gaaataatat
    16921 ctttaccaga aaaatatctt atatcagcat gttatccttc ttattacagt aaagataaat
    16981 ttataataag agtggaaaat ccaagtagta atagaatgat attggatgag caaatattta
    17041 aaaatagaaa tgggaaaatt gtaaatgcaa tagaagatgt agaagaagaa caggtatatg
    17101 aaatagcacc ttttgacgtt atttcaatat tattggattt ataagtgtaa aagtaagttt
    17161 attgtttaaa gtctgacaga tattaatctg ccagactttt taatttgtat aaatttgggt
    17221 aaatattaat gaatagtttt agtatttaaa tatgaattta gtatttaata tagaatttat
    17281 tgaagtagaa taattagttt aacaggataa gcagtttaaa taaagaatta ttatattaga
    17341 gtgattttat atataattta aaataataaa atgttaataa aatgagttaa agattgtata
    17401 ttaatagggt tgatgtagtt acaaaaaagt taccaattgt tatgtattta caaaactgat
    17461 atattaatta cataaaatct aaaatagttt gtaataggag tatatataat aaaaatttta
    17521 attaaataaa gctaggtata tttataatat taataaatat attgctataa aagtttgttt
    17581 aataaattaa ataaagaaaa gaggagaata aaatgtacaa catagatgat atgatatata
    17641 attgtccagt agaagcttta tcaaatattt taggtaaaaa atgggtggct ataattatat
    17701 ggaaaataca agaagataag aaacgatttg gagagctaca aagagctata ccagactgta
    17761 gtaagaaaat gttagttcaa caactagatt ttttaattga acaggggatt atagtcaacc
    17821 aaaaaaagtt ggttaataat gttgtagagt caacatattt tttaagtgaa tcaggattac
    17881 gattgcttcc tgtcatggaa aaaatgatta tgtggagcaa taaaaattta aattgtgata
    17941 aatactaaaa tttactctgt aagtaatgaa aatttaatta agctatgagt agaaaaatgt
    18001 attaaaatta cataattttt tagttagatt ttaatttaga aatgggattg taatattgga
    18061 aataaaataa ggaggaattt taatttgcaa aatgtaaatt tatttttagt atttatagaa
    18121 ggtatagtat cattcttttc accatgtata ctgccaattt tgcctattta tttgagtata
    18181 ttgtcaaata gtagtgtaga aaatctaaaa gaaggaaaga cgagttttat aggaagttct
    18241 ttatttaaaa atactatatt ttttgctctt ggaatctcaa caacattctt tatattgggt
    18301 tcatcagtaa aagtattgag tatgtttttt aatgaaaata aagatttaat aatgtttatt
    18361 ggtgggatta taattattat catgggatta ttttacatgg gaataattaa atcttcaata
    18421 ttgaatagag aaaaaagatt taatgttaag tttaaagaaa tgaaagcaat aacagctttt
    18481 atattaggtt ttacgtttag ttttggatgg acaccttgta ttggtccaat attggcatct
    18541 gtattagtca tggtttcaag ttcaagtaat catcttagtg ccaatctttt aatagcagta
    18601 tatactattg gttttatttt gccatttata attacagcta tgttttacag taaactattt
    18661 aaaactatag ataagataaa atctaacatg gaaattatta aaaaaatagg tgggattata
    18721 cttatagtat caggaatatt aatgatggta aatggatttg gaagtataag caaacacttt
    18781 aatacgtcac aaaatagcaa aattgaaagc aagcaagaag aaaataagcg agaaaattct
    18841 actgataaag aagaaaattc tgatggtaat aatagtcaaa aagactctaa taatgacaat
    18901 aatgataacg ggtctaatga tgaagataga ataaagtcta ttgattttac tttaacagac
    18961 cagtatggta aaacacataa attaagtgat tatgaaggca aagttgtatt cttaaatttc
    19021 tgggctacat ggtgtcctcc atgtaaagaa gaaatgcctt atatagaaca actgtataaa
    19081 gactataata aaaacaatga tgatgtagta attttaggag tagcttcacc taatttaggt
    19141 agagaaggtt caagggaaca tgtagttaac ttcttaaaag accaaggtta tacattccct
    19201 gttgtacttg atgaagatgg agcattagca tatcaatatg gaattaatgc ttttccaact
    19261 acatttataa ttgacaagga aggatatgta actcagtata ttccaggagc tatggataag
    19321 gcaactatgg catcatttat tgaaaatcag agaaataaat aaaagtattt aagtatatgt
    19381 ttatgaatca atttaaggtt acaaaaaagt gcctaattgt accacctaaa taatcttgtt
    19441 atagtaaaag tataaacaat acaagaaagg gttgaatttt atatgaaaaa aacatttgat
    19501 gctataataa taggatttgg aaaaggtgga aaaacattag caggagattt ggcaaacagg
    19561 ggattaaaag tagctttaat agaaaagtca aacaagatgt atggtgggac atgtgtaaat
    19621 gtggcatgta taccaacaaa aagcttagaa aattcagcaa acagcgtaaa aacaaagaat
    19681 ataaattctt gggatgaagt acaagctgaa tatgagaaag ctatagataa aaaagaaact
    19741 ttaattacaa aactcagaga agctaactat aataaattaa attcaaatga gaatgtaact
    19801 atattcacag gaatgggaac tttcattgat gaaaaaacag ttcaagttaa aactgaaaat
    19861 gaaatttatg agttagtagc agataatata tttattaata ctggttcaag accatttata
    19921 cctaatatta agggaattga aaataagaat atagtatatg atagtgagtc tcttatgaat
    19981 ctaagagttt tacctaaaaa aatgacaatt ataggagctg gatttatagg tttagagttt
    20041 gctggtattt atagttcatt tggagcagaa gttacaatat taaactctaa taatggcatt
    20101 ttaccaaatg aagatgttga ggattcagaa gaaataataa aattacttgc aaagagaaat
    20161 gtaaaaattg ttaataatgc aaatataaaa gagattaaag aagtttctga atcagcaata
    20221 gtagaatatg aagtagatgg taaatctaaa gaattaacaa gtaatatgat acttgtagct
    20281 acaggaagaa aagcaaacac tgaagggcta gggctagaaa atgcaggtat agaattaaat
    20341 gaaagaggat ttattaaagt ctcagaaact cttaagacta ataaagagca tatatgggct
    20401 ataggagata taaatggagg tccacaattt acttatatat cattggatga ttatcgcata
    20461 gttattaatc agttatttgg agataagact agaacaacta atgatagaaa gaatatccca
    20521 aattctatat ttataagtcc agcattttca agagttggtc ttaatgttaa acaagctaaa
    20581 gaaaagggat atgaagtatt agtggcaaag atgccagtag aagcaattcc aagagctaaa
    20641 cagattggca aggcagatgg gtttataaaa atagtgatag ataaaaaatc caataagata
    20701 cttggagcta gtatgatatg tgaaaattca agtgagatta tccatcttat tcaattagct
    20761 gtagatttag aagtagagta tacatattta agagaccgtg tttatgcaca tccaactatg
    20821 acagaagctc ttaatgatat tctatctcct aatatgataa aagaagtata acataagttt
    20881 aatatataaa cctaaaatta tcattataag tagattttag aaagaggcta tctcaaaaaa
    20941 tagagataag tctctttttt actttttaaa tgataaaaat atatttgatt ttataaaaga
    21001 agattatctt atgaactaaa aagtttattt taaaacacta cctatttatg ttataaactt
    21061 ataaatataa tatagaatct attattctgt attattaagt gaggttaagc gttataatta
    21121 attataaata ttgaataatc taaatggata agataatggt attaaaaatt atcttaaaag
    21181 gtgaaataat ctgtatttaa tgtagtacag ttatatcttt aaagcaaggt ggtgaaatga
    21241 tataaataag atatctaaaa gagggtttga tgatagtgat aaaagatggt tttcaagtaa
    21301 agaattaaca agacttataa aggctaaaga agaaatagaa tggttattaa atagggatta
    21361 caaaatagac ccagtcgtta cttttgttgg tgatagatat cagttttcta taagacaaag
    21421 agatgcatta aaaagagcag tctgtactga tgaaaaaaat attataagac aatcaaaaag
    21481 gctttttcta gataaaatca aagaagatac cgtaaatata gatggcttta atttgataat
    21541 atccatagag gtagcattat ctggtggtac tctagtaata ggaagtgatg gaaatataag
    21601 agatttagca ggtcttaggg gaacctataa gattatagat aaaacagaag aagctttgaa
    21661 tctgataggt aaatttttta ataaatacaa tgcacagaat cttaaatttt atttagatgc
    21721 tccagtttct aattcaggaa atttaaagta taggatatta gagcatgcca aaacttgggg
    21781 aattgaaact gaagtagaat tagtaaaaaa tgcagatgtt gtattggaaa aattagatag
    21841 agtagttagt agtgatgcag tcatagttga caagtgtata agttatttta atgtggctag
    21901 aggtataatt gaagagtata ttaaagaatg taatatcata aatttaaata aatagataaa
    21961 tagaggtgag aatatataag tatatgtagc aatattatta gtaatacaag taaagtttga
    22021 aaataggaga acgaatacat atcgaatttc ataaaatgtt gtaaaaagac atatgagagg
    22081 atggatatgt gtgaaattaa ctaaactaaa tattaaaaaa tatagagcac ctatatataa
    22141 atatgcttta gcaaatgtaa atttgaggtc tgctaaatct actaattcaa gtatcataac
    22201 agtaatacct cagggagcta aaatggaagt attggatgaa gaagatgact ggattaaggt
    22261 gatgtacaat tctcaagaag gatatgtgta taaggatttg gtatcagtaa gcgaatatgc
    22321 ttggagtaac ttaaacttaa gagaagataa atctataact tccaatataa ttacagtaat
    22381 tcctgaaaaa tctagggttg aagtccttca agtagatgga gattggagta agattgtata
    22441 tgatgataaa ataggatatg tttttaatta ttttttatca actgatggga ataaaccaaa
    22501 tgaattagat tataagtatt tttatactga catgattaaa tttgttaacg aaaataatat
    22561 taaaagtact agcgattatc taattgtaac agaccttaga aataaatata cttatatttt
    22621 taagaaagat aatggaggct gggggcaact ttacaagtgg caatgtacta taggcaagcc
    22681 tgagactcca actataacag gtatatttta tataagtgga agaaaacctt cttttggaac
    22741 ggatgagtat tcagttaagt atgctactag aattaaaggt ggatattact atcattctgt
    22801 gctatatgat tcaacaggaa gttatataat agatggcaga cttggtgaag ctcttagtca
    22861 tggatgtata agattaagta cggaaaatgc aaagtggata tatgataata taccagatac
    22921 aacaacagtt ataattcatt aagaaaaatt ttgagttttt attgggataa tgattctcaa
    22981 attaatagga ttatatatta acttcttcat aaagagtact atttaaaaag tgtaataaga
    23041 gagtgctatt aacaaaacaa agatattttg aggcactctc ttataaatat acataattaa
    23101 gatattttca taaacaattc tataatcttc tccttatcaa agaaaacagg attagcacca
    23161 gcacaagcat ctttcataga gttttctgca agcaattcaa aattaggatt atcaacacct
    23221 acatctttta aagattttgg gattccaacc tcttcagaaa gagcttttat cttatctatt
    23281 acaaagtcaa cacactcttt atctgattta ttatttacat ccattccaat aacttcagct
    23341 attggtcgga atttggcagg ggctgattta gcattttcct cctcaactat tggaagtagc
    23401 atggcattac atactccatg aggtaaatca tataaacctc ctaattgatg agccatagca
    23461 tgtacatttc caagacctgc attactaaag gcaattccat ttaaaaaaca agcataacac
    23521 atttgttctc tagcttctat gtcattacca tttttaacag ctcttggaag gtatttaaat
    23581 atctgcttta tagcatataa ggcagttgaa tctgtaacat cataagcacc attagctact
    23641 acagcttcta cagcatgagt tagtgcatcc ataccagttg ctgctgttaa attagatggt
    23701 ttgctaatca ttaattctgg gtcatttact gtcattgtag ctagtgagtt ggtatcaacc
    23761 attatcatct ttacatgtct gtcttcatca gttataacat agtttattgt aacttcagca
    23821 gatgtaccag cagtagtagt tatagctacg ataggaagac attttttaga agttttgttg
    23881 ataccttcat agtctctaat atctcctcca ttagtagcta atatagagat agctttacca
    23941 cagtcttgtg gagaacctcc tcctatagtt ataacgaagt cacaattttc tgattttaat
    24001 attttaagtc ctttatccac attagttacg gttggatttt gtttaacttc atcataaaca
    24061 acaaagtcta catctatctg agataaaata tctgttactt tttttacagt accatttgaa
    24121 actagaaatt tatcactaac aacaaatgct tttttagttc ctaatgattt aattgaatct
    24181 ttagcttcat taagacatcc tttcccaagt aaatttattg gtggtacata ataagtgctc
    24241 ataactaaaa tctcctttta ttttcattga tttttatgta aaaatatata tctaacaatt
    24301 ttcactaatt tactcataat atatcgtatc ttagattgag atagggttac tggttcttat
    24361 ataagaactt aattcgtaat tacgattatg attagaattt aacatagtat ttttatcatg
    24421 tcaataaaaa tataaaaaat attataaaaa aaacaaagtt actgtagtta aatcagcaac
    24481 tttgttttta tagtataaat caaataaata cattagttta aaacaaaatt tttaattatt
    24541 atgttttgat atcttccata tacagaatat taatctaatt tattacaatg tcttcattac
    24601 gttcttccat cattgattga atatttttct gagcatttaa gagtaatttt tttatcagtt
    24661 ctattttttc gtcattagct ctaaataaag gcagtgttac actaacagca gctcttatac
    24721 atccattttt tcttattggt acggcaagac attttaggtg ttccattgat tcttcacact
    24781 ctgaagctat ttgtgttctt tgaacttcaa gaagctggtc gtataaaaca tcaaaatctg
    24841 taattgattt ttctgtaaaa ccttttagac catctggata taagcttatt agtttttcct
    24901 tgtcaaaatc acataaaaga gcttttccaa gacctgtaca attagcaggt attttttttc
    24961 caacataaga aataagtcta attggttctg gagaatctac ttttccaata taaagaactt
    25021 cattattgtc aagtatacca agctgacaag tttcagaaca ggaattaact atattagtca
    25081 tttcgtcaat tatatagtta aaaatagttc ttttttcaaa aaatgaagac cctaatgtgt
    25141 aagaacgtat ccctatagaa tatttggatg tgtttttatc aatagtaatg tattttctag
    25201 cattaagtgt atgaactata ggaaagatgc tactttttgg agcattgata acatctgcta
    25261 tttctgtaag agtatatcca ttttcggatt ctgctaatgc ttctaaaata tctaatatcc
    25321 tagctgttgg gcgatgatta tctgaagtca taaatttccc cctcttttta ttaatttaaa
    25381 gtattagaat atgttgattt tagcacacta tacgatattt tacaatgtag aaaataaata
    25441 tgtaaattta tatataaata agctcttgat ttagatacag atagatattt ttttatactg
    25501 tacattaagg aataactaat ttccaaaata tacctaaaga aagtaagtga tatttagtta
    25561 tataatttaa gtaaatatat gcaatagtta tttttatatg taaatatcaa aatattatga
    25621 atttatattt tatctatttt ttacactgta taaaaacttt atagttttga atactatata
    25681 attttaaata cgctgtattt ttgcatataa ggaagtcatt tgataatata caaggtatta
    25741 tcagaaagtt tgaaaaaaat ttgacaaaaa tttatttttt ttattgactc ataaatagga
    25801 aaatgctatc attaattcaa agattcgtaa ttaaaaactt agttcttaaa tacgaaccga
    25861 aacggccgtt agaattaaag ctaaaaactt aataagagta attaaatatc atgttttagg
    25921 aggattaact ttatgataat ggattttatt aaaaaaattc ctgcaggtat gatgatagtg
    25981 ccaatgttta tagcagcttt cattaatacc ttttgcccag atattgtaca aataggttcg
    26041 tttacgacag cagtattttc aagtgttggg tctgcaacta taattggggt tcaactggta
    26101 tgcttgggaa ctcagcttca atttagagaa atgccaaaag ttttaaagag aggtggtata
    26161 cttcttggtt ctaaatttgc tataggtgct ttgataggta tacttatagg aaagctattt
    26221 ggaatggatg gtgtccttgg tcttacaaca ttggctgtta taagtgcagt tacaaatagt
    26281 aatggtagta tatatctttc attgatgact tcatatggag atgaaacaga ttgtgcaact
    26341 atgagtttac ttactttaaa tgatggacca ttttttacat taatagctct tgggacttca
    26401 ggtcttgcaa atgtgccatt gttgtcatta ttagctgcaa tagtgccaat acttgttggt
    26461 atgataatag gaaattttga caaaaaaatg aaagagtttt tagagccagg gtgtaattta
    26521 ctcgtacctt ttgtagggtt tgcacttggt agtagcataa atcttgcaag tatactaaaa
    26581 ggaggtttat caggagtatt acttggatta atatctgtat ttgtaggagg tatatttata
    26641 gtattctgtg ataaatttat aggaaaacgt ccaggatatg caggatgggc tgtagctaca
    26701 accgctggaa atgcaattgc tgtacctgct gctgttgcat tagttgaccc atcatggcaa
    26761 ccatatgttg caacagctac aacacaagtg gcagccgcag tagttgtcac tgcaatatta
    26821 gtacctctta taactagttg gtgggctaag aagtatggat gtcctaagtt cccaaaacaa
    26881 aatgaggatg gtacaataac agcttaattt atattacagt gagttgttgg taaaataata
    26941 aattctttaa ttagatttta ggatattaat aaaattatat tatttataaa ggagagcaag
    27001 aataatgata aatgcagtga taattgatga aaaagataat gttgcagtag ctattgaagc
    27061 aatagctaaa ggtaatgaaa ttagttttaa attaaaagac aaaacagtaa aaacgattac
    27121 agcactagat aatataacta tttatcataa attggctata aaagatatgg cccaaggtga
    27181 taaagttact aaatatggtg aacatatagg agaagcaaat caggaaataa aaagtggtca
    27241 acatgtacat attcataatg ttagaagtgt tagagaggat ttggataaac aagttatgac
    27301 taatatataa aataatattt atagagttat aaaagttgta ttcattgctt ttagtgtcaa
    27361 atagttttta aataggattt tagctgtttg attagtgagt gtaataaaaa ttataatata
    27421 taaaaattat aaaaagtata tagactattt taggaggaaa tcagtatgaa attttatgga
    27481 tataaaagac cagatggaag agttggtata agaaaccaca tattaatatt gcctacgagt
    27541 gtgtgtgcat cagatacaac tagaataata gcatcacaag ttcaaggggc agttaccttt
    27601 aataatcaga atggatgttc acaagtacat tcagaccaac aattaactat ggatgttatg
    27661 gctggttatg cagcaaatcc aaatgtatat ggtgtaatag ttgtatcttt gggatgcgaa
    27721 aattgccaaa atgatttggt agttgatgca ataaaagaga gaacaaataa gccaataaaa
    27781 actcttgtaa tacaagaaga acatggaact ttaaaaacta tagaaaaagc tgttcgttat
    27841 gctagagaaa tggcacaaga agcttctttg cttagaaaag aagaatttcc aatatctgaa
    27901 cttatactag gaactgaatg tggtgggtct gacccaacaa gtggattagc agcaaatcca
    27961 ttaataggtg aattaagtga taaacttgtc gatttaggag caacatcaat tttaagtgaa
    28021 actactgaat ttataggtgc agagcatata ctagctagga gagcagtaaa tgaagaagta
    28081 aaagaaaaaa tattacatat agtacataga tatgaaaatt cattaaaatt ggttggagaa
    28141 gaagttagag aaggtaatcc atctcctgga aatatagctg gtggtttaac aagtttagaa
    28201 gaaaaatcac ttggttgtat acataaagga ggtcatagac aaataagtga agtatatgat
    28261 tatgccaaac aaattgataa gaaaggtctt gttataatgg atacccctgg aaatgatgct
    28321 tcatctgttg ctggtatggt agcaggagga gcacaaatag tggtatttag tacaggaaga
    28381 ggaacaccat caggaaatcc aatagcacca gtaataaaaa taactggaaa taaaataact
    28441 tttgcaaata tggaagataa tatggatttt gatgctagtc cagttatcta tggtccacaa
    28501 acaatggaag aattaacaga tgatttgtta aatatggttg tagatgtagc aaatggaaaa
    28561 caatcaaaag cagagtccct tggatatact gaaatggcaa tagctagagt atgcaactat
    28621 gtataatctt taagtaagaa aataaataca tagaatttta ataaagggtg agattatgaa
    28681 gtttgtaagt tttactgaag gagaaaatga atgtaaaaga actggagttt tttctaaaga
    28741 tgaattattt attatagatg ttaactcttt aaatctcagt agaaatttta aggatttaaa
    28801 tgaattaatt caaaatgtat ctagtgaaga tattattaag ttgaaaacta ttataaatga
    28861 aagtatattc tataattatg atgtttttaa aagagagaga tgtactgtac atgttcctat
    28921 agaaaaacca atacatgata tcttatgtgt gggagtgaac tataaagacc atcttgaaga
    28981 aactcaaact cactttgatg atagttttga agagccacaa aaaacggttt attttacaaa
    29041 acgtgtgaca aaggcaatag gtccagaaga tgagatagat ggtcattttg agataaataa
    29101 tcaactagac tatgaagttg aacttggagt agtaataggt aaaacaggtg ttaatattag
    29161 aaaagaagag gtagaagatt atatctttgg ttacactgtt ataaatgata tttctgctag
    29221 aaagttgcaa aaagaacatg ttcaatggtt tagaggaaaa ggattagata catttacctc
    29281 tttaggtcca tgtattttac ataaaagtgc agttcctttt cccataaaac ttaaagtatg
    29341 tagtagggta aatggtgaag aaagacaaag ttctaataca ggattattca tatcagatgt
    29401 ttcagacata gtttcagaac tttcaaaagg tatgacttta gaggctggag acattatagc
    29461 tactggaaca ccttctggag tagggatggg attttcacct cctcgatata tgagtagtgg
    29521 agatgtagtt gaatgtgaga ttgagagtat tggaatttta aaaaattaca ttaagtaaat
    29581 gttttaatat aattaatatt aaagctaaat ctgatgtatt aatgaacata ttaaaataaa
    29641 ttttaggagt ttaaattatg aaagcagaat ttttgacacc agttgttaca atatttgatg
    29701 aagaaggaaa tttagataaa gaaggtaata aaaaagtgta cgaccatctt atagatggag
    29761 gtgtagatgg actagtcata atgggaagta ctggagagtt ctttaatatg tctatggata
    29821 tgcaaaaaga acttatagat ttagtaacta gtcatgtaaa taagagagta aaagtgttta
    29881 taggtacaag tcgtatgtgt ataaatgaat ctatagaact ttcaaactat gcacataata
    29941 gtggagcaga tggcgttatg ataataagcc cttattattt ctctcttgat gatgatagta
    30001 ttgagttatt ttatagtgaa atagctaaga atacagaggc tgaaatatat ttatacaatt
    30061 atccagatag aactggttat gatttaagtc cagaacttac attaagactt gcaagaaaac
    30121 ataaaaacat agttggatat aaggatacag taactttgat gggacatacc agagatttaa
    30181 taaatactat gaaaatagag ttcccagaat ttaaggttta tagtggttat gatgaaaact
    30241 ttatacataa tctaatgtct gggggagcag gttgtatagg gggattatca aatttaattc
    30301 cagaagtttg ctccaaatgg gctgaagctt acaataatag agatatggag aaggttatag
    30361 aaattcaaaa atatattaat aaagcaatgg atttttactt aataaccaat ccatttatac
    30421 cagcaatgaa aaaagctatg aatatcagag ggttacaaat aagtgattat atgactgctc
    30481 catttgtaaa accaaatgaa aaacaagtat gtgaaattaa aaaattgatg aataaattaa
    30541 atatatgtta aatcaacctt gcaaagaggt caacaataaa ttacaattat cttaataaaa
    30601 attaaattaa aataatacta gggtactata ttagtttaat agggaaagtg atgaaaattc
    30661 actacagccc ccgctactgt atacggatac gaaatcaaaa tccactgaaa tttataaaaa
    30721 ttttgggaag ggtgatggag gaaaagccgt gagtcaggag acctgcccag tattatataa
    30781 gttcaattta gggtggagat tgtaatttat aataaaatga agtatcaatt cgtttttgta
    30841 aagttgatat atgaacttct ccttattaaa ttaggagaag ttttttattt tattaaaagg
    30901 ttatacttat taaagatgac tttaggataa ctttaataag tatactttaa aaagcatata
    30961 ttattaaagt tttttagcca ctcataaaaa ataagatatt ggagtgaaag agatgaaatt
    31021 taaatcaaat gttttaaagc agtttttttt aattgctatg gtaattttta tggtaggagg
    31081 tcttataggg tgtagtaaaa gtgacaaaga gaataaacaa gctagtactg gtaagaaatt
    31141 agaattaaaa tatgctagtg gatttagtgt tgaagattta gaagatggaa ttaaaaaagt
    31201 aactgatgga gataatcgag aactgatttt aatcccaaag aagattaaaa ttcctgaaaa
    31261 atataaaaat gctaatatag ttagaacgcc tgtggacaat gtattaatag catctacaac
    31321 acaggcttgt tctctgagaa caatagatga actagatagt ataaaagccg taacaacaga
    31381 agagcagtac tggactatta aagaaattaa agatttaatg aaagatggaa aaatacatta
    31441 cgttggttca aatttagcac cagattatga aaaaatagta gacttaaatc ctgacttaac
    31501 tatagtgagt agtggtttat cagaggctga tacaaaattt attgacaaat taaaagaatt
    31561 agggcttaat tatgttgtag acaatgagta taaagagcaa aatccatttg gaagaatgga
    31621 gtggacaagt ttaatagctg cattttatga taaagaagat atggctacta gtgaactaaa
    31681 taaaactgta caaaaggtag aagaagtaag taaaaaaata aaaaacaaag ctaaacctaa
    31741 agttgtatgg gcatctgtat atgaaggaag tgcttatata gctcctaaag attcatatgt
    31801 ggataatatg ataaaaatgg ctggtggaat aaatgcatgt gcttcaatag attcaaatga
    31861 agggcgagta agtattgaac aattgcatga agttgcaaaa gatgcagata tatttgtata
    31921 ctcatcaagt tcagattatg caccaaattt agattctata aaatctagtg cacctgtatt
    31981 ggctgatttg gatgttgtaa aaaacggaaa cctatgggtg tttgctccag attattatca
    32041 atcagtagat aagacggacg aattaatagt agatttagtt gagatgttcc acccaggtac
    32101 tacaggtgaa aaaatcaaac attatataaa ttatgataaa tagtacagag aggacacaaa
    32161 aagataggtt ttatagattt tagttaatat aactttgcta agaggagtat acaaatggat
    32221 tttatcaata aaatgagaat aaggaatacg acaatattta tagtattaat tataagtatg
    32281 atacttactt ttttagtgtg tgtagcattt ggctctgtaa aaattgaatt taaagaagta
    32341 atagatgttg tattaagagg tatggataat acagaacatt ataaaataat atacaatata
    32401 agaatcccta gagtacttgc aaccatggta ggtggagcat gcctagcagt atcaggtatt
    32461 ttattacaga tattttttaa aaacccaata gttgaaccgt atatattagg agtatcttca
    32521 ggttctactt tatcagtagc tctcatgatt ttgggaggat acacattggg atttgagagt
    32581 gtaaattcaa tgagtatatt tgtagcagct tttataggag ctttaattgt aatgtctata
    32641 gtcattttat ttgcaaataa ggtaaagaat ataacaactc ttttaataat aggaattatg
    32701 accggatatg tatgtagtgg tataacaaat gtgttacaat catttgcaaa tagtgataaa
    32761 ttaaaagaat ttactatgtg gtctatggga agtttttctg gatttacatg ggaaaaggta
    32821 gaaatactta taatagttgg tatattaggt cttttgatga ctgtattcat aataaaacct
    32881 ttaaatgctt ttttattagg agaagagtat gcaaaaagta ttggcataaa tataagattt
    32941 tttagagttt tgatagtgtt tatatctagt atattagcag caatagtatc agcatttgca
    33001 ggacctatag ggtttattgg acttgcagtg ccacaaattt caaaattgat taaaaaatag
    33061 ctacgcacac ttgtgacttt agtcatgagt tagtagctaa aatagtcagc atatagggaa
    33121 acctatatgt agagatagga tagaacctat ccaatactac ttgaattgct ggaaacccct
    33181 aaagctaacc aaactaaaac ataaggatga aataaaccta agtgtgaaag ttacgaaagt
    33241 agaaaaaatt ggttagatgg ta
//
LOCUS       DAEPOZ010000015        32145 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.015, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000015 DAEPOZ010000000
VERSION     DAEPOZ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32145
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.015"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            188..1405
                     /locus_tag="KR440_000595"
     CDS             188..1405
                     /locus_tag="KR440_000595"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HBH0697661.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1500..1880
                     /locus_tag="KR440_000596"
     CDS             1500..1880
                     /locus_tag="KR440_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBH0697662.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2164..2646
                     /locus_tag="KR440_000597"
     CDS             2164..2646
                     /locus_tag="KR440_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0697663.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVNLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2667..3482
                     /locus_tag="KR440_000598"
     CDS             2667..3482
                     /locus_tag="KR440_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0697664.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3533..5083
                     /locus_tag="KR440_000599"
     CDS             3533..5083
                     /locus_tag="KR440_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH0697665.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPLLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5180..6532
                     /locus_tag="KR440_000600"
     CDS             5180..6532
                     /locus_tag="KR440_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBH0697666.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKERQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAESLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKVFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6663..7421
                     /locus_tag="KR440_000601"
     CDS             6663..7421
                     /locus_tag="KR440_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0697667.1"
                     /translation="MNIINKIFWQEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLIYNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELLNLLRTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7468..8187
                     /locus_tag="KR440_000602"
     CDS             7468..8187
                     /locus_tag="KR440_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBH0697668.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8311..9711)
                     /locus_tag="KR440_000603"
     CDS             complement(8311..9711)
                     /locus_tag="KR440_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBH0697669.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADIGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9715..10623)
                     /locus_tag="KR440_000604"
     CDS             complement(9715..10623)
                     /locus_tag="KR440_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0697670.1"
                     /translation="MKGDNKVTEKQRLIINIAQKIFDQKGFQNTSISDVVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWKNIFSRT
                     TINTYIRENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYF
                     SLHNKSLFFKTIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKETL"
     gene            10884..11531
                     /locus_tag="KR440_000605"
     CDS             10884..11531
                     /locus_tag="KR440_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBH0697671.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11856..14495
                     /locus_tag="KR440_000606"
     CDS             11856..14495
                     /locus_tag="KR440_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBH0697672.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHTEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMDTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14553..15656)
                     /locus_tag="KR440_000607"
     CDS             complement(14553..15656)
                     /locus_tag="KR440_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HBH0697673.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSSK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
     gene            16017..16838
                     /locus_tag="KR440_000608"
     CDS             16017..16838
                     /locus_tag="KR440_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBH0697674.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    16917..17128
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17215..18531
                     /gene="argH"
                     /locus_tag="KR440_000609"
     CDS             17215..18531
                     /gene="argH"
                     /locus_tag="KR440_000609"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBH0697675.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVGLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELHKFSDAFKEDIYDFIDYESILNKGIKKNLK"
     gene            18780..19118
                     /locus_tag="KR440_000610"
     CDS             18780..19118
                     /locus_tag="KR440_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HBH0697676.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGRP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(19245..20534)
                     /locus_tag="KR440_000611"
     CDS             complement(19245..20534)
                     /locus_tag="KR440_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBH0697677.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFFTGNNILMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            20614..21318
                     /locus_tag="KR440_000612"
     CDS             20614..21318
                     /locus_tag="KR440_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HBH0697678.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHNKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKPNQKNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            21410..22456
                     /gene="selD"
                     /locus_tag="KR440_000613"
     CDS             21410..22456
                     /gene="selD"
                     /locus_tag="KR440_000613"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:21458..21460,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBH0697679.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDNPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            22474..23886
                     /locus_tag="KR440_000614"
     CDS             22474..23886
                     /locus_tag="KR440_000614"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBH0697680.1"
                     /translation="MIVLSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVAELRKAFN"
     gene            24000..25883
                     /gene="selB"
                     /locus_tag="KR440_000615"
     CDS             24000..25883
                     /gene="selB"
                     /locus_tag="KR440_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBH0697681.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALEALISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            25988..28726
                     /locus_tag="KR440_000616"
     CDS             25988..28726
                     /locus_tag="KR440_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HBH0697682.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSIILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIISGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEYELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(28845..29789)
                     /gene="manA"
                     /locus_tag="KR440_000617"
     CDS             complement(28845..29789)
                     /gene="manA"
                     /locus_tag="KR440_000617"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBH0697683.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            29962..31323
                     /locus_tag="KR440_000618"
     CDS             29962..31323
                     /locus_tag="KR440_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH0697684.1"
                     /translation="MELFKAESKKYEKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSIVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            31411..>32145
                     /locus_tag="KR440_000619"
     CDS             31411..>32145
                     /locus_tag="KR440_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBH0697685.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIYCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYY"
BASE COUNT        12587 a         3587 c         5576 g        10395 t
ORIGIN      
        1 tatcacttaa tgtaatttaa atgaattgaa gatatatgtt tattgtatgc aattgttgta
       61 tttttaggtt ttttgtgatt aattaaaaaa ttataaaaat cgtttacatg ttgattttta
      121 aaaaaaaaag atatagaatt atatagaaag taataaacaa aaattggatt tacgggagtg
      181 gaatgaaatg atgaatgtta ccttaacaga tgtgaaagag gctagagaga ccattaagaa
      241 catagtaaaa agaactgact tacttgaaag tgttaaactt agcgaaaaaa caggagcaaa
      301 tgtgttctat aaatgtgaga atcttcaaaa aacaggttct tttaaattaa gaggagcttg
      361 taacaaaata gcgagtttaa cagatgaaga aaaggcaaat ggagttatag cttcaagtgc
      421 agggaatcat gctcaaggag ttgcattggg agcaaaaatg actggtataa aatctacaat
      481 agtaatgcca gcaactgcgc ctttagcaaa agttagtgca actaaaggtt atggagcaga
      541 agtagtttta aatggtgcgg tttatgatga tgcatatgca aaagctgttg aaatacaaaa
      601 agaaacagga gcaactttct tacatccatt caatgataag tatgttatag caggacaagg
      661 aactatatct cttgaaattt tcgaacaatt agacaataaa gttgatacta tactatgccc
      721 agttggtgga ggaggaatca tctctggtgt tgctgttgca gcaaaagcat taaatcctaa
      781 tgtaaaaata gtcggtgttc aaactgcaaa tataccttca atgaaagagt caataaaaaa
      841 tggaaaagta acaacagcat ttaatgatac tacaatagct gatggtatag ctgttaaaac
      901 tccaggagat ttaacttttg aaataataaa tgaattggtt gatgaaatag tagtggttga
      961 agagacagaa attgcagaat caatattatt tatgatggaa agtcaaaaaa tagtttcaga
     1021 aggtgctggt gctgtatgta cagctgctat attaagtggt aagtatgtac cagcaaaaga
     1081 tgaaaatgta gtttgtataa tatctggtgg taatatagac ataaatacgt tatatagaat
     1141 aataggtgtt gctttagcta aagaaggaag aagatattca tttagtacaa ttatggaaga
     1201 taagccagga aactttgctg agttaactag aataataagt gagaatggtg gaaacatttt
     1261 aagtgctaat caaggtaaat tatcagcagg ggaagctcta ggaaaacaaa gtgcggaatt
     1321 tatattagag acaatagatt atgaccatat agctagaata aagaaagcta tagaagaaaa
     1381 aggttttaaa attatagaat tataataaaa agtgtatata atatacaaga aaaatagtat
     1441 aatagtgtat gaagatacat tttatgtgta ttagattaac gaattaggag gattttaaaa
     1501 tgaaacatga agtaatacat actaatgatg caccagcagc tttaggacca tattctcaag
     1561 ctataaaagc tggaaactta ttatttgtat ctggacaagt tcctcttgta ccagagacta
     1621 tggaagtagt tgaaggagat gtacaagctc aaactgctca atctttaaaa aatcttaaag
     1681 ctatattagc tgaaagtgga gctgattttt caaatgtagt taagactaca gtatttataa
     1741 aagatatgaa tgaatttggg gctataaacg aagtttatgc tgaatatttt ggagaaaata
     1801 aaccagcaag agcttgtgta gaagttgcta gattaccaaa agacgttaaa gttgaaatag
     1861 aagtaatagc tgtattataa aaatacaata tttttaattt gaaaaggctg aaaaactata
     1921 ttctagtgat tcagtctttt tataaaaaaa taaatcacaa taaagaacat gataaatatt
     1981 gtcgaattaa aaaataaaat taaaataaat attgcaatat ttttgagaag gttttataat
     2041 gagtatataa tttgaaacgt gtaactatta aattaggcat gagttcttgg atatttttat
     2101 cctgaggatt catgcctttt ttgtattgaa attcaaaaat ctatttaata aggaggaagt
     2161 attgtgttaa attttttcaa gaaaaataaa agctataagt tacatgcagt agtgagtggt
     2221 aacagcatta atattgagaa agtaaacgat tctgtgtttt caaaaaagtt aatgggagat
     2281 ggagttgcaa ttattcctaa tagtaatgtt gttgtagccc cttgtaatgg taaagtaact
     2341 gtgcttacag aatcaaagca tgcctttggt atggtctcag atgagggtgt tgaaatatta
     2401 gtccacatag gaattgatac agttaattta cagggtgagg ggtttaaaaa tgaagtgtct
     2461 caaggagata ctgttaaaaa aggtagtcca ataataagtt ttgaaagaga aaaaataaat
     2521 tctcagggaa ttgattgtac aacaataata attgtattaa atcatagcga attttcagaa
     2581 ataaattgca tggtagaaaa tgaagttgtt gcagggcaag atactgttat tgaaattatg
     2641 aaataatagg aagggattaa ttcaatatga gagtagtaaa aatctttaat aataatgcat
     2701 tatctacagt aactaatgat aggaaagaag ctattttact aggtcttggt ataggtttta
     2761 ataaacgtcc aggagataaa gttaatgaag ataaaataga aaagatttat tatgttcaag
     2821 accacatgca aacaaaattt ttagaattat taaaaaatgt tagtccagaa gtgatggatg
     2881 cttcacaaca aattataagt cttataccag gaaatgaggg aaaatttaat aataaaggga
     2941 tattatcttt agtggaacat attagttttg caatagatag aatgaaaaat aatgtttttt
     3001 tacctaattt aatgcttttg gatataaaga tgatgtattc aaaagagttt gaattaggag
     3061 taaaagcttt agaaattata tatagagtat gtcatatcca tttgcctgaa gatgaagcag
     3121 gttacatagc tttacatttt gtcaatttac aatcaaatga taatttggca tatgatactt
     3181 taaaatttgt aaaaggtagt attgatttga ttaaagaatg ttatggatta gaattagacg
     3241 aatcgagttt aagtacatta cgttttagaa ctcatcttaa atttcttgct caaagaattt
     3301 ttaagaatga aatttgtcag gatgataaaa tgattgaaat gtatgattat ttaattaaca
     3361 atcatcctaa aaataaagaa tatttagaaa agttaaatgt atatattgaa aaggagttta
     3421 ggtataaatt agataaacct gaaaaaattt atctactact acacctaaca aaaatcttat
     3481 aagaatgaga tgaattagta atagaaatat ataattttag ggggggataa gattgaaaga
     3541 aaaactactt ggaggactgg aaacatttgc aaaggcaatg gttcagccat taatgtattt
     3601 atcgagtgca ggaatattaa tggtggtagg tgtactactt acaaatgata tgttattagg
     3661 agtacttcca ttttttaaat gggagccaat tcaaatattt ggaagcctaa tttataactg
     3721 tataatggct attattaata atcttgctgt tatatttgct ataggtattc ctgctgcact
     3781 agcaaaaaaa gagaagcaca aagcagcgtt aataggattt atgagttata taatatacct
     3841 tacagcttcc aacactatgt taacatcact aggtcaacta gcaaaagaaa gcaagatgct
     3901 tggactgata gggactggac aagctactat tttaggtatt caagttgtag atacaagtgt
     3961 atttggtgga attattttag ggtgtatagc tggatatgta tttaatcgta caagcaacaa
     4021 aacatttaaa ggagctttac agatatactc gggagcaaat tttagtttta tatgcatgtt
     4081 ctttgtatcc attttaattg gagttgtgag taactttata tggccattag ctcaacaagg
     4141 aatagcttct cttgcaaatg tcatcaaaga tacaggtaca tttggtttat tcttatatgg
     4201 ttttttagag agaatattag taccaactgg acttcatcat ttaatctata caccatttca
     4261 gttttctgat ttaggaggag tgcttaaagt tggtgcagat acagtttcag gagcctatgc
     4321 tatagttatg acagaaatga atttaccagt tgagcgtttc tcagatagta tatactacat
     4381 ggcaactgga tttacaaaga tgtttggtta tataggtata ggagctgcct ttatttatac
     4441 tgcatataag caaaataaga agaaagttaa ggcaacgatt attccactaa taattacggc
     4501 agtattagcc tctgtaacag aaccattaga ctttatgttt gtattctgtg caccactttt
     4561 attctttgta catgcagtta tagctggtct ttttatagct ttattaaaaa tatttgatgt
     4621 tactgcatta tgtggaggga acttattagt atcaattata atgaatacta cagtaggtgt
     4681 agaaaaaaca cactggccag taatgatgat attaggactt gcacaaatta ttgtatattt
     4741 tgtagtattt agttttttaa ttaagaaatt taattataaa actccaggtc gtgaagatga
     4801 gagtacagct acaggcgaag aacaatcaaa acaacttaat ttagatgcag gtatagaaaa
     4861 tataattgat gggttaggcg gaaaagaaaa tattaataca gtagaaaatt gcatcactag
     4921 attacgtgtt aatgtaaaag atgaaagtaa gattaatgaa gatataatta atttaacgcc
     4981 aaatagcggg attgtaagga aaggaaaaga tatacaaata atttttggac ttcatgtgca
     5041 tgaggtaaga cgtgcagtag aagatttttt agaagcttat taaagatata tatttatata
     5101 gcaagggtaa ataacagctt taagtgctgt ttactattta ttaaagatac atataattta
     5161 tagaggagga ataattatta tgattattac tattgttggt ggaggaagca catttactcc
     5221 aggtattgtt aagtcaattg cgttaagaga aaaagagtta gagcttgagg aaattcgctt
     5281 atttgacatt gataaagaac gccaagataa agtagctgta gttgtcaact ggatactaaa
     5341 agaagatgtt aaaacagatg ttaaactaac tgtaactaat tgtgaaaaag aagcgtatac
     5401 agatgctaca tttatatttg cacaaatgcg tgttggtaaa tatgaaatgc gtgagcagga
     5461 tgaaaaaatt ccattgaaat atggttgtgt aggtcaagaa acttgtggtt gtggagggat
     5521 ggcatacgga ctgcgtacaa tacaaccaat gataaaggtt attgatgatg tagagaaata
     5581 tgctaaaaaa gattattgga tattaaatta ctctaatcct gctgcaatcg tttctgaagc
     5641 ttgtcgtaag ttaagaccaa atgcaaggat tataaatatc tgtgatatgc caatagctat
     5701 tatagatatg atagcggagt cattaaatat caatgacgtt cataatatac gctatgatta
     5761 ttttggtcta aatcattttg gctggtttac ttcaatagat tataaacata gagacttaat
     5821 ggaagagatt aaggaataca taaaagaaaa taaaatatta ttaccaaaat cttatataga
     5881 aaacttaaat aaaagtcgta gtacaggaaa tcgccatgct gtaagtagct ggatacatgt
     5941 ttgggaatct gtttatacaa tgttagaaag cttcccaaaa tatctgccaa acacttattt
     6001 aacatactat cttttaagta aagaaacagt ggaacattca aacataaatc gtactcgtgc
     6061 aaatgaggtt atggaaagcc gtgaaaaaga cttatttgaa ggtgtgagac attacttaga
     6121 aactggagaa atttctgaaa aagtatttta tgcaggttca catggagatt ggattagtga
     6181 tttggcagtt tcaattaaaa atgatacacg tagtcgtttt ttagttataa ctgaaaatcg
     6241 tggagctata ccaaacatgc catatgatgc aatggtagaa attccagcat atattggtaa
     6301 agatggtcca gaagttattg ctcgaggtcc aatacctcta ttccaacaag gattaatgat
     6361 gcaacaactt aacagtgaaa aattgatagt agaagcttat atagaaaata gttatgaaaa
     6421 agctttacaa gcattcacat taaataaaac tattccaagt atgaatgttg ctaaagaaat
     6481 tttggatgaa atgatagaag ttaataagga atactggcca gaattaagat agtaggataa
     6541 aagaaagggt ttttatttaa taattagata tgttttcaga gtcttttatc aaaatgtatt
     6601 cacaaacata tggcaatata ttaaaataaa gagtgatatt tttagaagaa tggggtgatg
     6661 caatgaacat tataaataaa attttttggc aagaagatag gattggcatg attacaaata
     6721 atttagaggc agatacacat agccactgca tgttacaact gttcttaggg atagaagaca
     6781 gcattgaaat aacagtcaat gaaaaattag ttaaatgtaa ttgtatcata gtagataaaa
     6841 atatctctca ttcattttca gcaagaaaaa aagtttatta ctctgctatt attgaaccta
     6901 catctatata tgcagaacag cttacctcaa aaatgaatga ttttggctac tggatatgtg
     6961 acaatgatgg attagagaaa ttacgtcaac aaggaacttt tttgatatat aactctagta
     7021 aagaacaata tttaagattt atggaaatgc ttaataacta tttgaatata cctataactc
     7081 tcaaacatta tgatgacaga attactgaat tgctcaattt attacgtact tgtaattgtg
     7141 ataatcatac tatttcaagt tttgcagata aagtgtccct atcagcaagt agattatcac
     7201 atctgtttaa agaacaaata gggatacctt taaaaagcta tatcttattt catcaaatgg
     7261 agtgtgcttt tagagaatta ctttctggga aaaatgttac cgaagcttct atgactgctg
     7321 gattcgatac tccatcacac tttgcaggga cagttaagcg aatgatggga atgcctgttt
     7381 ctttatcttt gaaagatagc gaatttttga aagtatatta aatattctcg ttatataatg
     7441 aagttgtact aagaaagggg ctttgttatg aataaagaat atttagagga aacaaaaatg
     7501 ttaaattata atgaaccaca acttaagctt ttggtatcat caaagaattg gttggaatta
     7561 gatgattttc ataaaataaa atctatttat gagtttgtac agaatgatat tttatttgga
     7621 tataatgctt ctgatatgtt aagcgcaaca caggtattaa atgatggtat gggacaatgc
     7681 aatacaaaag ctactctttt aatggcatta ttacgtgcag tgaatatccc ttgtaggctt
     7741 catgcttttg atgtaacaaa agattttcaa cgaggtgcta catccaaatt gatttcacta
     7801 ttagcaccaa aatatattct tcatacttgg gtagaggtat tttatcaaga taggtggatt
     7861 gctctggagg gtgtcataac tgataaaaaa tatttagaag caattcagaa gaagtttttt
     7921 aatcatggtg gaacgtttaa aaagtatgca atagctacaa atgatttgaa aaatacatct
     7981 attgattggg atggtaaaga tacttttatc caaaaagaag ctatcgtgta tgattatggt
     8041 atttttccat caccagatgt atttttttct actcattcac aacatatgtc aaaattaaaa
     8101 aatttcatat atgtacatct tatcagaaaa ataatgacaa aaaatgtatg taaggctaga
     8161 aacaattata ttgataaaaa cgaatgagtg tttaagaggt aactatatag gaaatatata
     8221 gaataattaa aactgtactg gttaaaatga ataaaaagga gttatcatag agcaaactta
     8281 aattgttcat aggataactc cttttctgta ttaacttcta ataatttgat gtctttttat
     8341 aaaacatata gatacaatta aaccagtaaa tgcaagagta gctgctattg aaaatgcaac
     8401 atctattcca tgaatgttag acagcatgtt tgaactcata tgagctttct tagtagcact
     8461 tgtcattata gtaataagta tagcagaacc tatagctcca gatatctgac ggagtgtgtt
     8521 attaattgct gtggcatgag atataagttc tctatctaat gttttaattc cccatgtagt
     8581 taatggcatt aaaaccattg aaataccaat catacgaata caatacatca atgacacatg
     8641 aattaaagat gtatctctgc ctaaaaaagc gaaggataaa gttcctaaaa acaaacatcc
     8701 tgttcctaat attgatagag catgaggtcc atatttatct aaatgacgtc ctgcaattgg
     8761 attgagtaca accatcaata tagcaccagg taacattaat gaacctgaag ataatgcaga
     8821 aaatcctctt acagattgaa cgtacagagg aatcattaat gtagcagaca tcattgaggc
     8881 atatacaatc ataattaata ttgtagaaat aagaaatggt ttatttttaa aaaccctcaa
     8941 ttctaaaaaa ggttctttag attttaattg acgcaatgta aataaaatta atgacataat
     9001 tcctattaat attggtaaat atgtggcaat attagtccac ccataattac cttgattagt
     9061 aaatccaatt aataaaccac caaaacctag agatgatagt actattgaag gtatatctaa
     9121 ctttagtttt tgagtttccc caacatttct taatagaata aatgatagta taacatctaa
     9181 tatagcaata ggggaaagaa acagaaataa actatgccaa ccaaatgaat ctactaacca
     9241 tcctgataaa gttggaccta ttgctggagc aaatcctact gtaataccaa ctaaactcat
     9301 agctgcacca cgtttttcct cagggcataa atagagtata ataacttgta aaagtggcat
     9361 tagagaacca gaacccattg cctgcaacac acgagcaatc aacatagttg aaaaattatt
     9421 agaaaaaagt gcaataatac atcctataga aaagatagac attgatgtaa taaataactt
     9481 tctagtacta aatcgattta ttaaataagc tgttgtgggt acaataatac ctgcaataag
     9541 tagataaatt gttgttaacc actgaccaat atcagcagta attttaaaat ctgacattat
     9601 attaggtaat gctgttgaca atagtgtttg actgagagaa cttataaaag caccagtcat
     9661 tacaattgct attattaaat ttcctttttt attgttactt tgggtttcca taatttataa
     9721 cgtctccttt tttaattgat ttttaatgct gttcaataaa agtacttttg gtcttaaagt
     9781 gtcttctttt tctaaatatg caatcattgc atccataatt aaagaatcat attgttgatt
     9841 ttttccctcg acgtataatt tttttattgc ttctagtaat ttaggatttt caaggagaga
     9901 agtatcagtt ttgattagtt cttctatatc ttgtatcgtt ttaaaaaata gcgatttatt
     9961 atgaagggaa aagtaaactt catcttcttg tttatgtaaa aacattgatt tttcaattaa
    10021 aggatttaga ttatccatat tttctacaac taaatctaga atttgtatag taaatttggc
    10081 aataaagtat acatttattt ctcttttttg tacatgaata atgtatataa actgatgaat
    10141 tagagcatcc aataagaaaa ctaaatcaaa tataattttt tctattttat caccatagtt
    10201 atcaaataag cttaatttgt attcattcaa taagttagct cgtgattttg tttgcaattt
    10261 acttgtagcc ttacgttgct cttcagaaaa attttctctt atataggtat ttatggtagt
    10321 tctagaaaaa atatttttcc atacagcaat tatctttctg tttaactttt ctgatgatgg
    10381 aatgtcatta ttttcgttta tggaatgtat aatttctaaa aacttttcat cataataatt
    10441 gattatttcg cagataaaac tttccttagt ttcaaaatgc ttgtaaaaag tagctttaga
    10501 cattttacac tcttttacaa catccgatat tgaagtattt tgaaaacctt tttggtcaaa
    10561 aattttttgt gcaatattta taatgagcct ttgtttttct gtcactttat tatctccttt
    10621 cacgcaaaaa cgtttagtac tatatagtac ctacaagttt taataaatat attatacatc
    10681 atttggaaaa aaaatcaata tatagactgt aatatgaaat ttttgaaaaa tttgttgtta
    10741 tttttagtcg tataaaaaag aaacttaaga gtttcttaag aattgtttaa agaagttgca
    10801 ataaattagt ggtatgatta tagtatgaaa gagaatgtct tgaaattttt ctgtatcaaa
    10861 ttccaattat ggaggtgtag tatatgaaaa agaaaatttg tgtagcaata atttttgctc
    10921 tatttatagt ttttgtagaa atgtttattg atagctctag cgaaggaagt aaaattatga
    10981 cagctgaatt aagtaaatac aggaaaggcg taaatgtatt atcaataaac aatattatag
    11041 tagcgaataa aaaatatagt ttgcccaaag attattctcc tcaggaaagc agtgaagcga
    11101 gagatgcttt ttataaaatg aataaagatg ctcaaaagtc aggattaaat ttaaaagctt
    11161 ttagtacata tagaagttat gaatatcaag atagattatt taaatcatat gtaaaagaac
    11221 atggagaaaa agaagcaaat agattttcag ctaaacctgg agagagtgag catcaaactg
    11281 gacttgcttt tgatattgga ggagatgacc agtcttgttg ggcaaataaa aagttcaaca
    11341 atacaaaaga agctaagtgg ttgtatgaaa atgcatataa atatggtttt atacttagat
    11401 atccagaagg taaagagaat ataacaggat atatgtatga atcttggcac tatagatatg
    11461 ttggaactga acatagcaaa aactttgcaa tgaataattt gactttagaa gaatatttac
    11521 atataaatta agagatggtt tgttaaaatt aggttaaaat aaaaaataac ctagactatt
    11581 agctgtgtaa agtgatataa tgtaaataca atttaagata actactcgaa ggggagtagc
    11641 ttacacgaaa gtggtaatta atcgtcaaga cagtagaaat actcggttaa ttagcaaggg
    11701 attccttgat agcaagacct tcaaaataag cattaggtat gtttgttttg gggtcttttt
    11761 ttattcaagg aatttttatt taaaaaaatt taaatttcaa gtatacataa gagcccccaa
    11821 acataaaata aatttattga agaggagggc aacaaatgtc ttacaaaagt acttatcaag
    11881 aagtaattaa aaatttagac tcaagtttat ctggtttgag taaagaaaga gcagaagagt
    11941 tgttggaaaa aaatggagct aatgaattaa aagaagcaga taaggttcct acttataagc
    12001 ttttcctaga aagttttaaa gacccattag taataattct attgatagct gctttagtac
    12061 aaatattttt aggtgaaaca gtggagtcta taataatttt tgcagttatt attataaact
    12121 ctgttctggg tgttgtacaa acaaaaaaag cagaaagttc attagaaagt ttgaaaaatt
    12181 tatctgcacc aaacgcaaag gtaataagag acaataaaaa aatgacaatt ccagcaaaaa
    12241 atctagtagt tggagatatt gtattcttag aagctggcga ttacatacca gcagatggaa
    12301 ggcttataga agcacaatct ctcaaggttg tagaagggat gttaactgga gaatcagaac
    12361 cagttttaaa gcatacagaa aaaataagtg aagatgtaag tttaggtgac cagaaaaata
    12421 tggtatttag tggtgctgta gttgtttatg gtagaggttc ttttgtagtg actggaacag
    12481 gtatggatac tgagatgggt aaaatagctg ggcttttaga aagtgctgaa aataaactta
    12541 caccattgca acaaaaaata gaggatttta gtaaaaaact tggtgtaggt attgtaatac
    12601 ttgctctttt aatatttata attcaagtag caagaaatta ctttatggct ggaaatggag
    12661 aaatgacaca aattatttta gattcattca tgttctctgt agcagtagca gtagcagcta
    12721 taccagaagc tctatcatca atagtcacta tagtattggc tgttggaaca aatagtatgg
    12781 caaaaaaaca agccataata agaaaattac cagcagtaga aactttaggt tcaacaagtg
    12841 taatatgtac tgataaaaca ggtactctta cacaaaataa aatgacagta gttgattatt
    12901 atatgtatgg aactaataat gatgagttag ataaagaaaa agtaaactat tcttatcatg
    12961 ctaaattatt gactatggtt tctacattgt gtaatgactc tcatataact agtgatggta
    13021 aagaagttgg tgacccaaca gaagtggctt taataaatta ttcaagtaaa aatgatttag
    13081 attatgataa attaagaagt aagtatatca gaataaatga aataccattt gattcagaca
    13141 gaaagcttat gtctactgta aatagtatat atggagatta tataatgttt acaaaaggtg
    13201 ctccagatat tgtgtttagt agatgtaaat atgctttgaa aaatggttca aaagtagata
    13261 taacagatga gataatagaa gaatataaac ataaaaatga agagttttca aatagagcac
    13321 ttagagtatt agcttttgca ataaaagatg tcccagaaga agattttata ccttcaattg
    13381 atgacgaaca tgacatgaca ttggttggaa taatggcaat gatagaccca ccaagagaag
    13441 aagtaatgga tgcagtaaaa gaggcaaaga gtgctggtat aaaaactgtt atgataactg
    13501 gtgaccataa aacaacagca gcagcaattg caaaagagat tggtataatg gaagaaggag
    13561 acttggctct tactggaaaa gagcttgatg ctttgagtga agaacaacta tatgaaaaac
    13621 tagaaaatat aagtgtatat gccagagtct cgccagaaaa taaaattcgt atagtaaaag
    13681 catggcaaca taagaataat ataacagcaa tgactggtga tggtgttaat gatgcacctg
    13741 cattaaaaca agcagatata ggaattggta tgggaagtgg tactgatgta gcaaaagatg
    13801 cttctgctat ggtattggtt gatgataact ttgcaagtat agtaaatgct gttgaagttg
    13861 gtagaacagt atacagtaat ataataaaat caattactta tctatttgca ggaaacttag
    13921 gagctatagt atccatagta tttgcagtgt ttgcaggttg gtcaaatcca tttacagctt
    13981 tacaactttt gtttataaac ttagtaaatg actctttacc agcaattgca ttagggtttg
    14041 aaaagtcaga aaaaaatata atgaataaac ctcctagaca cccggatgaa agtatactag
    14101 caggaggaac attgaaggca gttgctataa gaggttgtat aataggtgta gttacaataa
    14161 ttgcacaata tataggatta caagtatctc cagaacttgg agtagcaatg gcattttcaa
    14221 cattgatatt agctagaata atgcaaacat tacctgcacg ttctaataat caaacagtaa
    14281 taaaattagg ttttttcagc aataaatacg ttataggagc agttttagtc tgtcttgggc
    14341 tttactcttt aacactaata ccagtgctaa gaggaatatt ctctatacca acaacgtttg
    14401 gattgtacca gttggggatt tgcctagggt tagcattaat ttcatcaata ttaatggaac
    14461 ttaacaaatt aataaataga agtgaagtaa aataatcaaa aaattaaagg gcatattgat
    14521 tttaacaata tgccttttat aaatagtcta atttattcta tattatcgat tatttggaat
    14581 gcttttataa cttcaggtaa ttctttttct tcaggcattc taaatcttat gaaattttta
    14641 tcaagtccag taaaatcact tccagatata actcttattt ttaatttagc aaactcttgt
    14701 tgtaaatcta tgtccttgtt tttatgttca actgtcataa ttgatacagt atcactagtt
    14761 tcagctatat taaggttttt ccatggaatt agtaattgat tttttatatc ttttgttttt
    14821 tcttttaatt cttctataaa ttgaacatca tctaataaat ttgctgcaat actacgagat
    14881 atttcagaca cttcgtaagg agtagaaatc tttttaatag ggctaacaag ttgttctgga
    14941 agaactgcat atcctgctct tagtcctgct aaaccaaatc cttttgaaaa tgtttttaaa
    15001 gcaattacat tatcataatt atttagaagc ttgacagctg aattttcttt tggcatgtat
    15061 tcaccataag cttcatctac cataactgct atatcatatt tagctgcttc ctttactata
    15121 ttttcaattg atgataaagg tataatttgt cctgtaggat tgtttggatt gtctatgtaa
    15181 attactttat attctggatt tattttttca ataaattctt tttcattaaa cttgaaatta
    15241 tcctctttct ttaaaagaac ataatcataa gttgcaccat gcatttttat atctgtctca
    15301 tattctgaga actgaggtga atatcctaaa actttatctc ccttttcaat aaatagtcta
    15361 tttaaaaggt aaataacatg tatagaacca tctgccaaac aaattctgtc agtatctaat
    15421 gctataaaat ctttccaata atcgactata ttatctttta aagcaattga atgaggatat
    15481 cctcttatca tatcaaattt taaagcatta aaagcttcta cagctttgct tgagtaagac
    15541 acggtattta taccttcacc acagtctatc tcgaatacat cttctgaatg tccagattct
    15601 attgcataac tttttttcaa ctgcatttca atagctttgt taatttttag tttcatactt
    15661 tatcactcct tatttagtca ataataaatg ttataaaagt tgctagattt aaaatattat
    15721 gaaaatatat aatagtaaaa aatgaatata aatattataa tagctcactt tatttaatat
    15781 gattatatat catatattgt aaaatttcta gcattataag actataaatt gaaaatattc
    15841 agaaaaatac tacaagaaac gatttatttt gctcttaaaa taagttaaat gaatgttatt
    15901 aattttgtaa attgaaatag tttaagtata aagttatgat ataatctaat aagggtaatt
    15961 ctttagatta tattaaggaa atataggtat acaataaaaa gttatggagt tggattatgg
    16021 gatataaatt aattgtaaca gatatggatg gaactttatt aggcaataat cataaagtta
    16081 cagatgaaaa taaaactgca ctacaaaaag ttataaaaag cggtattaat gtaactttag
    16141 caacaggaag agccttcgat tcagctaaat gcaatgtaga ttttcttaaa gaggacatgc
    16201 caataatagc ttgtaatggt tcattaataa gagagcaaaa tggaaatata atatattcaa
    16261 ataagattga tacaagaaca tgtttaaaca ttttagatgt attagataaa tacgatattt
    16321 actaccaatg taatagtata gattctatgc tttcgaaaaa aatagaagga cgtgaagata
    16381 gattaagtgt atttttaggc tctgaaacag aagtaattgt aaaagatgat ttgagagaag
    16441 aaatctttaa aaaagatata ctaaaatttg taatcataga agaaaaaaat ccatctattt
    16501 tggatgaaat aagaaaagaa ttgagaaaag ttcaaggaat aaagataact tcttcatggc
    16561 caaacaatat tgaagtcatg aatgaaggtg ttgataaagg taatgcagtt aaaattttag
    16621 ctgaaaaaat gaatatagac agagaagata taattgcatt tggagacaat tacaatgaca
    16681 tagaaatgat aaagtttgct ggtcttgggg tagctatggg aaatgcagaa gaattgatta
    16741 aacaagaagc tgattatgtt acagatacaa atcaagacag tggagttgca aaggcaattt
    16801 ataaattcct agagttaaaa gaatcactta gctcttaaaa cagtatataa taggaaaaaa
    16861 taggttgact attttgtaga tagtaattat aatacaaata gataaaacat aaaaatttag
    16921 attcagtgaa aaagagagta gatatagaga aaatctcagc gaattggggg atggtgtgag
    16981 cctaatgata acactttata ttgaaaagaa ctttggagaa tactacttga aattcagtat
    17041 ggtagtaggt aatctcgcct taagagaata gagtgggtaa acattgttta ccaactagag
    17101 tggtaacacg gaatatttcg tctcttggtc tttttgacca agagattttt tagtttcatt
    17161 gagttacaag acttataatt aaaatacaaa aaattaaaat atgaggtgaa agttatgaaa
    17221 ttatggggcg gacgctttag aaaagcggaa aatcaactta tggaagaatt taataaatct
    17281 tttggctatg attgtgtact gtacaaaaaa gatatcgaag gaagtgtagc acatgttcat
    17341 atgcaagtaa aatgtggact tttaacagaa gaagaaggaa agtctataac agaaggttta
    17401 aaagggatac ttgaagatgt agaaaatgga aagttagtat tggatggaga gtatgaagat
    17461 atacatagtt ttactgagat aaatttaatt caaagaattg gagatgttgg caagaagctt
    17521 catacagcac gtagtagaaa tgaccaagtt gcagttgata tgagattgta tgctaaagaa
    17581 aaagcaaatg atttggtggg tttaatatct gaatttaaag ctactataaa agatgttgct
    17641 gacaaaaacc cagtgatgat gccaggctat actcatcttc aaagagctca agtagtgaca
    17701 tttaagcatc acttaatggc atattatagt atgtttgata gagatgagaa aagaataaaa
    17761 aatgctatag aaatattaga tgaatcacct ttaggctgtg gagctttagc aggaactacg
    17821 catgatatag atagaagtat aacttgtgaa caattaggat ttaaaaaagt agtagataat
    17881 tttatggatg gagtaagtga tagagattat ttactagagc taatgagtga tttttcaatt
    17941 ataatgatgc acttaagtag attaagtgaa gagttgatac tttggagttc tcaagaattt
    18001 ggatttgttg agatagatga tttatataca actggaagta gtataatgcc tcagaagaaa
    18061 aatccagatg gtgcagagct tataagaggt aagactggca gagtatatgg aaacttattt
    18121 ggtcttttta cagtaatgaa aggaatacca ttagcatata ataaagacat gcaagaagat
    18181 aaagaaggtt ttttcgatag tgtacatact cttgaaatgt gtatacaaat aatggataga
    18241 atgatagcca cactaaaggt aaatgaggat aaaatgaaac aagcagtaaa aaatggtttt
    18301 ttaaatgcaa cagaagttgc ggattatttg gttaaaaata atgttgcttt tagagatgca
    18361 catggtatag taggaagtat cgttatatat tgtgaagata ataaaaaagc aatagaagat
    18421 ttaacattag aagaattaca taagttttca gatgctttta aggaagatat ttatgatttt
    18481 atcgactatg agagtatatt aaataaggga ataaaaaaga atttaaaata atgtatttat
    18541 ttttaaaagc tgtaaatttg aatgttatca tttaaattta cagcttttta ttgtgtaaga
    18601 ttttattata taaaagtaag aaacttaaaa ggagtagtat ttaaaataaa tacaaataaa
    18661 aaattattat taaaatgata attaatatca ataagtattg acaaaattat ttctaaatac
    18721 tattatatat aatataaata ataatgaaaa tcaatatcaa ataaatggga ggaaatctaa
    18781 tgaacttatt agtgattggt ggtcatgaaa gaatggaaaa agattatatg gttatggcca
    18841 agaaaaaagg ttataagaca aaggtatata ctaccatgtc atctaaatta aataattcta
    18901 taggaagacc agatgcagtt gtcatactta catcaactgt ttcacataag atgtctagaa
    18961 cggttgagtc tcaagctaaa aaacaagata tattaatcgt aaggcataaa aatagtagta
    19021 aggtagcatt taatgaatgt ctagatatgg ttgatgaatg tctgggaaat tgttcaaaat
    19081 gtaagcttaa agacaaacaa aaacaaaaaa atatataggt ataaaaaata cataaacata
    19141 aaattaaaag tactgagata atagttataa ttattatctc agtactttta gatttataag
    19201 gataagtata ttataaattt tttattataa tttccaacta cttgttactt tttcatattt
    19261 tttttacttc tagctttttt tctttttttc ttctttatat ttgaagctat tataaataaa
    19321 actaaaagca ctagaatacc tgcaataatc ttaacaaaag tcattaaaat attatttcca
    19381 gtaaaaaatg gaagactgct atcaagatta ttttttgcaa ttaagtctat actgttttct
    19441 agtttaccat tattatatac atttaaagta ccaaccttag ctcctttttt tataggcaaa
    19501 tcaattttat ttaactctgt tttggtatca taattgcctt gagattcatc tttttctaag
    19561 aatatcctat aactattttt tggttcataa atcaattcat gttgttttgt aaacaatact
    19621 tttttagtat ctgtgtattt ttctttatca acaatagtag cacacttaaa gttatcaaat
    19681 ccataatcaa tcagaatacg tgagtctaaa tataaatcag cattagttga attaaataca
    19741 gcaacgataa ctcttctgcc atcttttaca gcactcgata aaagacattt accagcagca
    19801 tcagtatatc cagtttttat accatcaact atatcatatt ttatatttat aggttgacct
    19861 ttgtatgtaa tcttatcatg tgatgtcaaa aacttattag tgtttacaaa gtatctctca
    19921 taaggatatg cttttgtagc ttcaaattta agtgacttag tttttacaat ttgtctaaaa
    19981 gtatcattat tcatagcttc tctagctatg agagccatat catgagctgt tgtgtagtga
    20041 tttgggtctg gtaatccatg aggattatta aaatgagtat tagtagctcc aatttttttt
    20101 gcctcagagt tcatgaggtc aataaatttt tgtacattgc caccaccaac atatctagcc
    20161 aatacaaaag cagcatcatt agcagaatga accaaaagag catctagtaa ctgctttaca
    20221 gtaaaagatt ccccattttt tagatacata ctagagccat ctatttgagg taaatcttta
    20281 atttctatta cttgatttaa atctttaacc tcctttagaa ctaaacaagc tgtccatact
    20341 ttagttgttg aagctggata aagcttagaa tttccatcct tattatatag tattttgcca
    20401 gactcatagt ccattaaaac tgcatgttta gcaactatag ctggttcatt atctgcaaaa
    20461 gagatattag agcttcctaa aaatgaagta aatatcaata aacagattaa taaaagtgat
    20521 aaatttcttt tcattgtgag cctccaaatt ttaaattcat cttttagtat agcatatatt
    20581 tgtcaataat taaaagaaag gagtgattaa tatatgaaaa aaaaggtaaa atttatttcg
    20641 ttttttgttt tatttatttt aataatatca tcaataatcg tttataaatc gcttattttc
    20701 aaagatgata tttatgttgt aagtaaaaaa aatttaccta atgaaaaaac acatgttgta
    20761 gagaatgaaa ttaaaggtga agataataat cacaataaag taactaaaga catagataaa
    20821 aattttcaaa ataataaaat aactatatac ataagtggag cagtaaatag acctggaatt
    20881 gtaactatag agtcagacaa gagactttat gatgctgtag aattactagg aggaacgaca
    20941 aaagaagcag atttaaatgg agtcaatcta tcagtaagat tagaagatga acaacattac
    21001 ataataccta aaataggtga agcaacatct gttacaagta atgatgattc caataaacca
    21061 aatcagaaga atgagtcaag aggtgagcct aaaaataaat ccaataaaaa tatatctaac
    21121 gaatcaaaag taaatataaa tgtagcgact atagaagagt tagattctct acctggggtt
    21181 ggagaagcta cagccaataa aattttacaa cacagagaag aaaatggaca gtttagttca
    21241 attgaggaaa taaaaaatgt aaatggtatt ggagataaaa agtatgaaaa tataaaagat
    21301 ttaatttgtg tagactaaaa tataatacaa aacttacata tagatatgtt attataaata
    21361 tgtatgtata tgtgtgtaca tgtggaaatt tagtaaagga ggcactttta tgaaaaaaga
    21421 acaaaaaaaa cttacagaga tgacaacagc aggtggttga gccgctaaaa tagggccaga
    21481 ggttctggca tcagttttat ctcaattgcc taaaaatgat aatatagaaa atttattagt
    21541 aggactagat acagcagatg atgctgcagt ttataaatta aatgatgata tggcacttat
    21601 ccaaacttta gacttcttta caccaatggt agataaccca tacgtatttg gtcaaattgc
    21661 tgcatcaaat tcactatcag atgtatatgc tatgggaggc aaacctttgg ttgctatgaa
    21721 tattgtttgt tttccatcat gccatgatat ggatgtactt gcggaaatat taaaaggtgg
    21781 gtttgataaa gtgaaagaaa gtggagcact tttggttgga gggcatactg tagatgacaa
    21841 agagcctaaa tatggtcttt cagtttctgg gattgtaagt ccaaataaag ttttatcaaa
    21901 tgctactgca aaacctggtg ataagttaat tataacaaaa ccaattggag tgggtgtatt
    21961 aaatacagcc atgaaagaag gtatggtaga acaacatata gcagataaag taatagaaat
    22021 aatgatacat ttaaataaat atgcagcaat gagttttgag aaatttgatg taaattcggt
    22081 tacagatata actggttttg gtctacttgg tcatacttta gaaatggcca aagcttctga
    22141 agtaagtatt gaaattgaat caaaacatgt accaattatt gaaggtgcaa tagaaatggc
    22201 acaaatggga attatacctg ctggtatgta taaaaatatg cattatgtat ctaaagatgt
    22261 agaagttgta ggaaatatag aagttgcagt tcaagatatt ttatacgacc cacaaacatc
    22321 aggagggttg ttaatttcag taaaagaaga acttgcagag gaacttgtaa aagatatgaa
    22381 attaaatgga gcaatagaag ctaaaattat aggttctgta gttccaaaag gggaaaaata
    22441 tattacagtt ttataaaata ttatagagaa agagtgattg ttttgagcaa aagagaattg
    22501 tttgcaatgt tgccttcagt tgatgaagta ttaagtgata atagaattgt agaaataata
    22561 aatgaatatc ctagaagttt agttttagaa tctgtgagag aagtaataga tttaaaaaga
    22621 caatttatat taagattaaa agaagatgca tcaaactctg taacaataga gtttggtgaa
    22681 ataatagaat ctgcaataga gagagttaag ttaaattact cattatcttt aaaaaaagtt
    22741 ataaatgcaa ctggtacagt tattcataca aatttaggga ggtctttact tagtgaagat
    22801 ataaaagatg aattatggtg tgctgcttca aggtattcaa acttagaata cgatttggat
    22861 aatggagaaa gaggttcaag atactctcat ttaactagta caataaaaag acttactgga
    22921 gcagaagatg tactagttgt aaataataat gcagcggcag tacttcttgt acttagcaca
    22981 atggctaaag gaggagaagc aattgtatct cgtggagaat tagttgaagt aggtggttca
    23041 tttaggattc caagtataat ggctcttagt ggtgcagaat tagttgaagt tggttcaact
    23101 aataaaactc atttgaaaga ttacaaggag gccattacag aagatacaaa tgtattgatg
    23161 aaggttcata caagtaatta tagaataatg ggattcacag agagtgtttc tatagaagaa
    23221 ttggttaatt taggtaaaaa atataaacta cctgtaatag aagacttagg aagtggagtt
    23281 tttatagatt tatcaaaata cggtttatct tatgaaccaa ctgtattgga ttctattcga
    23341 caaggtgcag atgtagtaac ttttagtgga gataagatgc ttggaggtcc acaagcaggt
    23401 attatagttg ggaaaaaaga gtatatagaa aaaatgaaga aaaatcaact tactagagca
    23461 cttagagttg ataagcttac aatatgtgct cttgaagcaa cattgagaat gtatttagat
    23521 gagacaaaag ctatagaaaa tatacctact ttaaaaatgt tgacatataa aattgaagaa
    23581 ttagaagtaa aagcaaataa actatttgaa aaaataactg ctttaaactt aaatgcaaac
    23641 ataaatatag aagatggatt ttcacaagtg ggaggagggt ctatgcctct tgaaactata
    23701 agtacaaagg taatatcaat aactcctgaa catatgaatg tttcatcttt agaaaaaaaa
    23761 ctaagactta gtgaagcaca tataatagcc agagtgtatg ataataaata tgtactagat
    23821 gtaagaacta tatttgatga tgaatttgat gtaatagttg cggaattgag aaaagcattt
    23881 aattaaataa atttgagcta ataaatatta aatttagaag tataagtatg aagttttgat
    23941 taaaaaatag ctacacacac ttgtgacttt agtcatagca ggtttagagg gggcaaaaaa
    24001 tgaaaaatgt agtaattgga acagcaggtc atattgacca cggaaaaact actcttataa
    24061 aagcattgac tggaagagag actgatacat tagatgaaga aaaaaagaga ggtatttcaa
    24121 taaacttagg atttacttat tttgatttac caaacaatag aagagctgga attgttgatg
    24181 tcccaggaca cgaaaaattc ataaaaaaca tgttagctgg agcatcagga attgatatag
    24241 ttttacttgt tgtagcagca gatgaagggg ttatgccaca gacagttgag catttagata
    24301 ttctaagctt tttaaatatc aaaaatggaa tagtggttct aactaagtct gatactgtag
    24361 atgatgaatt tagagagcta gtaaaagaag atatttgtga aaaaataaaa ggaacttttc
    24421 ttgaagatgc tgaaataatt gaagttgatt ctatatctaa aagaggtata gacactctta
    24481 ttgaaaaaat agataaaatg agtgatgata tagaagataa aaatgaaaac tctccagcaa
    24541 gacttaatat agacagagta ttttcaataa aagggtttgg tactgtaata acaggcactt
    24601 taatagaagg aaagataagt atagaagatg atttagttat atatccacag gtattaaaaa
    24661 caaaaataag aagtatacaa gttcatggag aaaataaaga tacagcttat gcaggtcaaa
    24721 gaacagcaat caatatatca aatgtaaaag tggatgatat aaaaagagga gatgttttag
    24781 ctgcaccaaa ctcattagag gaatcaatga tgttagatgt aaaattatca ttggtaaatc
    24841 atttagataa aggattaaag cactgggata gattaagatt gtatcatgga actagagaga
    24901 tactttgtag agcagttcca ctagataaag aattaattga aagtggagaa agtggctatg
    24961 ttcagttgag acttgaagaa agcatagtat ctaagaaagg tgatacgttt gtagtgagac
    25021 gttattctcc aatggaaact ataggtggag gtgtaataat tgacccatct cctaaaaaac
    25081 ataagaaatt tgatgaaaaa gttatagaag cattaaaaat aaaagaaaaa ggcgaactaa
    25141 aagatataat agaagagtac ttaaaaagga atttaaaaaa ttatccaaat ataaaagaaa
    25201 taatgagcta tagtggtgct catgaagaag atgtaaaaag agcgcttgaa gcacttatat
    25261 cagaagataa agtatttatt ataggaaata tgtatatgca tataaatcaa tacaataaat
    25321 taaaagaaaa tacaataaag cttttaagtg agtaccataa gaagtatagg cttagaaaag
    25381 ggatactaaa agaagaagta aggtctaaga tagagagtaa ttttaagact agggagatgg
    25441 atattttatt agaaaaattg tctacagaaa atgctataaa aatagaaaat aatatagtat
    25501 ctctattaga ttttgaggtt attttaaatg ataagcaaaa agaaatagca aaaaaaatag
    25561 aaaaaagatt aaaatcatgt ggagtttctt ctatactgac aatagatgaa gtttctgaag
    25621 gaaatcataa ctatgctgaa gtattggagt caatgatagg taataaagta gagaaattag
    25681 atgatttgta tataatggac aaagatattt atgaaaatgc aaagaacatt ttgataaatt
    25741 atataaaaga aaataaagaa ataacattag gagaatacag agatttgatt gattctagca
    25801 gaaagaattg catgattata ttggaaaact tcgatagaaa caaaataact aaaagagtag
    25861 aaaacaaaag aattttgttt tagcagaaaa tgtctgtttt tgctttaagg tataaagaag
    25921 gaattataat gtcgagtatc gtataattaa ttagtataaa atattaatta agaggaagtg
    25981 taaagtattg aagaatataa tacctgatgt aaaaccagat ataaaagaaa gagaagacat
    26041 gagcctttta tttataataa tgggctcagt acttttatta tttattataa aagatataga
    26101 gaaatatgaa actttattac atgccatata tacgatattt ttagtagcta caggagtaat
    26161 tatatttaat actacaaaat ttaaagtaga ttcattttct acatttttag gattaatttt
    26221 tatagctact ggagtattgg agtgtatata tttatttaat tccttgggga ttaaaactaa
    26281 gtctataatg gagattaaca tcacaatatc agcaataaca gatttatttc ctatactggg
    26341 tgtatactta agttttaaat ttgttaagga taacaaacaa atttattcaa gtataatttt
    26401 atttatagca actgctacat taacaataac atctttattc ataatatcag gaatttgtga
    26461 ctatctggga aatggtaaat cacaatatgc tttaggagtt ataatatcta tattcataat
    26521 aattatttct atcattagtg gaattgaatt aaattcaagt tcaaataaag gtaaatggga
    26581 atatgatgaa aaaaagattt ttaacagaat aatagtaata ataattttat caagagttcc
    26641 aaatctattg catatagtta ttgataatag aaatgtagag cggatattat cacagataat
    26701 aataaatgta gccttatatt atttatataa ttacatagta tctaagaata ttaagaaaac
    26761 agcactggaa ttgcatgata cgaatgaaga attaacaaaa aaaacggaaa gtttaaaaga
    26821 aaagaataaa aaattaatct atgagacaga aaagatagag gaacttaagg gaatacttat
    26881 gaaaagagaa tctaggctac aatctacatt agatgtagca gtaaatagca tagtagtgtt
    26941 tagcaaagat ggagaaatta catatgcaaa taagagcttt agaaatactt ttggagacta
    27001 taaagaacaa gataaattga ctgataaaat aaaaaacttc aatgatttga tagaaagcat
    27061 tcattatgtg tttgttcatg agaagaatgc agagaaactt attagtacaa gcgataataa
    27121 agtttatcaa gctatatttg cacctttaat tattgcttca caaacagaag gagtattgtg
    27181 tgtactagaa gataagacta agaaaaagga gtacgaacgc aaacttattg aagcaaataa
    27241 gagaagtaaa gattttttag aaagtattgg agatggcata gtagtgcttg aaggtaataa
    27301 caaaatatat gttaataatg catgtaggga aatttttaaa aataatttag agagtattga
    27361 tttttcatta gtatgtaaaa gtgaagaaag tatagaaaaa agatatgtaa tagatggtga
    27421 agtaaagtat gtagagatga gtttttctca gtatactaat gctggtacta ataaaactat
    27481 tattgtaata cgtgatacaa cctctcgaaa aatatctcaa ataaaacttg aggaaagtca
    27541 atcatcatat tcaagattta tagatatatt acctgatggc atatgcttat taaaagaaga
    27601 tttaagtata aactatgcaa ataagtcgtt tttagatatg ctagctttta caaacataga
    27661 tgatataaaa gattctaata ttaaactatt aatgaatgcg aatactgaag aaaaaatgaa
    27721 atttacggat aatatggcta aagttttaaa tgaaaataga agtatacttt tactggaata
    27781 tgaattgata aatagttatg atgatatagt agaagtagaa ttaagtgcat taccttttgc
    27841 tatatacaat actagataca taatgcttat tataaaagat ttagtacata agaaatattc
    27901 cgagcaagca gaaaaagaac tgcttgaaag atttaaaaca gataaaatca aaacggagtt
    27961 ttttgcaaat atgtctcatg aattaaaaac tccactaaat gttatatcta gttcaaatca
    28021 actggtagat tctttctata gaaatgaaaa gataaaagat tacaataaca atattaaatc
    28081 tcatgtggat ttagttagac aaagttctta taggcttcaa agattaataa ataacattat
    28141 agatttaaca aagatggagt ctgggttcta tacactaaaa cttgcgaaat acaatattgt
    28201 atctgtaata gaagacttat ttatgaatat agaagagtat gcacttagaa aagacataaa
    28261 aattcttttt gatacagacc ttgaagaaat taatgtttat atagacaagg ttgaaattga
    28321 gagaattatg ttgaatctat tgtcaaactg tattaagttt actgataatg gaggctggat
    28381 atatgtaagt attcattata aaatagataa agtaatcata agtgtaaaag acactggagt
    28441 tggaatacca caagataaat tagaattgat ttttgaagaa ttttcacaag ttgataagac
    28501 tttatccaga aatactgaag gaagtggaat agggcttgct atagttaaga atctagtaag
    28561 tcttcatggt ggagatatag aagtagtaag tgaagtgaat aaaggcacag agttcttaat
    28621 ttcacttcca atgaagggat ttagtagtga acattacaca gaagataaga gaatatataa
    28681 tattcaagaa aaaataaaaa tagaattttc tgatatatac tattaaaaat gattatgagc
    28741 aaaaataaaa taaaaatact atttgtttaa atatttaagt tgtaagaaat aggctatcat
    28801 aaggtaaatt attatgatag cctattttta gctaggtttt atatctatat gtgagaacat
    28861 ataatctcta aattaccttc aaaactaaaa tctccaaagt cgtttggcag tataaaatgc
    28921 ataccctttt ttaaattata ttctacagaa ttgtgtataa gttttccatt tccatccaac
    28981 acactgtata aagaaaaagg tgtattgtgt gtaaaattac attttccaaa aacatctaat
    29041 ttataaacac taaaaaattc gtttgacaca aatgttgtgc atttaaagtc tgatgttgat
    29101 acgattttat agtcagtatc gaaatttata tgaggaacat tcgtaacatc tattgatttt
    29161 tgaacatgaa gttcacgctt attacctgaa ttgtcagttc tatcataatc atagactcta
    29221 taagtagtat ctgagttttg ttgagtttct aaaatcaatg ttcctttgca tatagcatgt
    29281 atagtaccac ttggaacata gaaaaagtct ccctttttta tattaacctt tctaagtagt
    29341 ttgtcccatt cattattatt taccatattt acaaattctt tatgagattt tgcattatga
    29401 cctattataa tttctgcatc atcagaacaa tctattacat accaacactc tgtcttacct
    29461 aactctccat tttcattttt cttagcatat tcatcatttg gatggacttg aactgataaa
    29521 ttatcatttg catccaatat ttttgtaagt aaaggaaact tatcaccagg tgtgtttcca
    29581 aataattctc tatttttatt ccacaattca gatagttttt tacctttata ttttccattt
    29641 tctattaagc aatctccatt tttatgagag cttatagccc aacattcacc agttgttggc
    29701 gaatctattt cataattaaa tttatcttta agtgcagtac caccccatat tctatccata
    29761 aaaataggtt ttaaaaataa tggttccata atcctgttcc tcctatgata ttaaatttat
    29821 cctacaaata attatatata aatagattat aaaatcaata gtaaaaaagt tttgtccctt
    29881 gaaatgacat aaagtataag gagttattaa ataagataaa ctatgatata taatataaag
    29941 ttctaaattg gagtgataat tatggagttg tttaaggcag aaagcaaaaa atatgaaaaa
    30001 gaatttttaa aattattgga gcaatgggtt agtatacctt cattttatga tagaaaaact
    30061 gtatctaaag atatgccttt tggaaaagga gtatatgatg cattaaactg gtttgaaaat
    30121 ctaggtagag aaaacaattt caaagtaaaa aatatagata atcatgctgt tcaaattgaa
    30181 tatggaaatg gtaaagaata tgtagatata tttggacatt gtgatgttgt aaatccaggt
    30241 gaaggatggg atagtgaacc atttaaacta aatattatag gtgataaatt agttgctaga
    30301 ggtgtatctg ataataaagg acctatgata gtgaattttt tagcactaaa aatgataaag
    30361 gatttagata ttaatttaaa aagaaaggtt agattaatag ctggaggaaa tgaagagagt
    30421 ggatttaaat gtataaagca ttattatagc aaagagccat atggtgtatg tggatttact
    30481 ccagatgcta aatttcctgt attaaatgga gaaaaaggtg gagcaataat aaagttaatt
    30541 tcaaatatag acgataaatc tttatatata agtggtggaa tagagtttaa tacaatacca
    30601 gataaagttt atataaagaa tgtagaaaaa ttgggtaaag ataatatatg ttttgatatt
    30661 aacaatatat cgataaatta tgataatggc aattatattg tgtatggaaa aggtggtcat
    30721 tcatctaaac ccgaaaaatc aataaatcct atattagcta caattaaact tttgtcagaa
    30781 aatattgatg aaaaatggac aaaagattta tataagttga taaatcaaga taatataaat
    30841 ggaaacttat ttggtttaaa tattgagggg aaatgtggga ttttatcaat agtgccaaca
    30901 ataataaata tagtagatgg aaaattagaa gtagttttaa gcgttagata tccagaaata
    30961 ttaaccattg aagatataat aaaaaaattt aatttgtata tggagcaaaa taatattaat
    31021 aaatttgagc ttattgggga aaatttaaag caagcaaatt atatagatag aaattcaaaa
    31081 cttgtaagaa gtttgcatga tatatatata aagtattctg gagatttgaa aaatgatgtt
    31141 agagtaacta gtgcaggaag ttatgcttct gaaatgaata attcagtaat atttggttgt
    31201 gaatttcctg atggtagctt tggcaatgtc catagtgcga atgaatttgc tagcttagat
    31261 agatttataa ctgcaatagg tatatatgca gaggcaataa ttacattatg taataaaata
    31321 taaatacaaa atgtccctta tatagacata ttatatatac aattaaaatt ttacttgata
    31381 tataattgaa atcaaagaga aggtgataca atgataacta agaaacatgc aataatagta
    31441 aagagtttaa gcaataaaga tggatatatg acatcaaacg aattagctat caaattggat
    31501 gtgtctacta aaactattaa aagatatata gcagatttaa atagtgtttt aagcaaatat
    31561 gatttggtaa tagcttcttc tagaggtata ggatacaagt taagcggttc taaaaacaat
    31621 atagccagag ctgttaaaga agcaaataaa tatattgatg gttttttaga tgactctgaa
    31681 gagtcaagaa tgtcaaatat aatatgtatg cttattaata gaaattatat gagtatagaa
    31741 gcaatggcag aagaattaaa tctaagtata gccgctataa ataaattgtc tagtaagctt
    31801 aaaagaaaac ttgaaaagta tgacttagtt ataaaatcta aaccttacta tggaagctat
    31861 atacatggtg aagaaattaa tataaggcaa ttaattactg actatgcaat aaaattagat
    31921 gaaaaaagta aagtgaaggt gtttttagat gacattagtg aaaacgatat atactgtata
    31981 gaaagtattt tagaaaagca cttaagagaa aaagatacaa taatttcaga taaagatttt
    32041 aatcttttgc tttcaaaaat aattgtttct gtatttagga gtaagagagg tcatagtaac
    32101 aatataaact taatggatac aagttataga tttcataact actac
//
LOCUS       DAEPOZ010000016        31748 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.016, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000016 DAEPOZ010000000
VERSION     DAEPOZ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31748
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.016"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            9..326
                     /gene="flgB"
                     /locus_tag="KR440_000620"
     CDS             9..326
                     /gene="flgB"
                     /locus_tag="KR440_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBH0697686.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYVTFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            334..738
                     /gene="flgC"
                     /locus_tag="KR440_000621"
     CDS             334..738
                     /gene="flgC"
                     /locus_tag="KR440_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HBH0697687.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            748..1065
                     /gene="fliE"
                     /locus_tag="KR440_000622"
     CDS             748..1065
                     /gene="fliE"
                     /locus_tag="KR440_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBH0697688.1"
                     /translation="MPITQINNLMQGAKLDNLIGETKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            1090..2640
                     /gene="fliF"
                     /locus_tag="KR440_000623"
     CDS             1090..2640
                     /gene="fliF"
                     /locus_tag="KR440_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HBH0697689.1"
                     /translation="MIASFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDVQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAIPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDEDVDENNQPTNSLINVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSATNNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKAEAKQELEEIKKEGFKKNIIKAVIGVVAVVAIGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            2653..3723
                     /gene="fliG"
                     /locus_tag="KR440_000624"
     CDS             2653..3723
                     /gene="fliG"
                     /locus_tag="KR440_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBH0697690.1"
                     /translation="MSTEITNVNAFDLSDINESDKKEKIEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNAKANKLLEGISINTSTKPFKHIIKLDVKKILEILRDESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSISGDDFKDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQDPALAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAADALKEEMEMVGVIKVTQVEAAQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            3716..4447
                     /locus_tag="KR440_000625"
     CDS             3716..4447
                     /locus_tag="KR440_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBH0697691.1"
                     /translation="MNNKIIKSKKASYKGVLDLTIIDKVRVGESNVNKENDCRFRDEV
                     IKEAIESAEIKKNEILEEAKMNAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYTQKA
                     KDEADFIKNQADLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESMSDL
                     LKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGNAII
                     QKENGRIIVGIDCAMEKLKEELLGE"
     gene            4451..5770
                     /gene="fliI"
                     /locus_tag="KR440_000626"
     CDS             4451..5770
                     /gene="fliI"
                     /locus_tag="KR440_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HBH0697692.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGIAIG
                     EPPAQKGYTPSVFAILPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRIFE"
     gene            5802..6248
                     /gene="fliJ"
                     /locus_tag="KR440_000627"
     CDS             5802..6248
                     /gene="fliJ"
                     /locus_tag="KR440_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HBH0697693.1"
                     /translation="MELDKNFKFRLQKVLDLKIKDEEEIKMEFAKIQQKKIDIESNLE
                     NLESNYSKYSISKNNDSVQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEEQLKEQSTNDEFASMSYLRNRQVL"
     gene            6276..7481
                     /locus_tag="KR440_000628"
     CDS             6276..7481
                     /locus_tag="KR440_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HBH0697694.1"
                     /translation="MKIQADSYNIIKDIGNRKSSSKNKNIKNNKFEDNLYKATDHKKV
                     KDRRETSKQNVSSDKRENSDNECHLDEDKSNHLEDENTYEKDCSNNNEALHQLLNLLK
                     DNYKKDKDINTILNNLKKLSISDELKQELKLNKNILNKIANQNLELNRASTNNPNDIE
                     NLDIMKLRELLENNASEDIEVISKTTENDTKSIDLYSFDSNRIDTLNSNKQKDDSSDI
                     LEKLAGVNGNKSGNFGQVINKSVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSKQNTNDKRNNVVIEDEIKENII
                     DDDRVDLLA"
     gene            7499..8107
                     /locus_tag="KR440_000629"
     CDS             7499..8107
                     /locus_tag="KR440_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HBH0697695.1"
                     /translation="MYGIATALAGTQVNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMND
                     KLDILLALSNSTLINSALSMATSLIGKEVEVSGEEEEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVGVKYEKLLKIGEVDANSGEENDSSEQESPEEV"
     gene            8134..9117
                     /locus_tag="KR440_000630"
     CDS             8134..9117
                     /locus_tag="KR440_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HBH0697696.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMTQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGVIYDGEYKADT
                     GEFIKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GNIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            9130..9333
                     /locus_tag="KR440_000631"
     CDS             9130..9333
                     /locus_tag="KR440_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HBH0697697.1"
                     /translation="MIRVTDLNGRRYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKVNVGYIRSEK"
     gene            9333..10151
                     /locus_tag="KR440_000632"
     CDS             9333..10151
                     /locus_tag="KR440_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HBH0697698.1"
                     /translation="MKKTDSLTFIGLILSTVLVLFGAVKGSSSGLKSFFDFASILITV
                     FGSFGALMITFTIDDIKLIKDALQYSFKTMNISKLDLLNQFKTLSKKARREGLLSIES
                     DVMKIQDPFMKKGLELCIDGLEVNEIRSILELEIDSVEETNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLSDLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            10141..10836
                     /locus_tag="KR440_000633"
     CDS             10141..10836
                     /locus_tag="KR440_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBH0697699.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGISMFSTGSSTSEKSPIESVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKADSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPRLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            10848..11291
                     /locus_tag="KR440_000634"
     CDS             10848..11291
                     /locus_tag="KR440_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HBH0697700.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQIDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            11306..11680
                     /locus_tag="KR440_000635"
     CDS             11306..11680
                     /locus_tag="KR440_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliO/MopB family protein"
                     /protein_id="HBH0697701.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYIKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMNKIKDLTKEEILRIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            11681..12346
                     /gene="fliP"
                     /locus_tag="KR440_000636"
     CDS             11681..12346
                     /gene="fliP"
                     /locus_tag="KR440_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBH0697702.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLKQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            12359..12628
                     /gene="fliQ"
                     /locus_tag="KR440_000637"
     CDS             12359..12628
                     /gene="fliQ"
                     /locus_tag="KR440_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBH0697703.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMMSLITK"
     gene            12640..14454
                     /locus_tag="KR440_000638"
     CDS             12640..14454
                     /locus_tag="KR440_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HBH0697704.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGINETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLIDNLMNWTARMIFFSMDGHHVLIRGIRYSFELIPIGKSFVDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIIGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSTDKTEEATPKKKGEQRKKGNIAKSK
                     ELPVAMTLLAFTLLVPTLFSYVVDTLKSSLNYFLSLDFYMNINYSSIEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIVANLFQVGILFTGETLKPNLSKLNPVSGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIVIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEIKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVLKR"
     gene            14463..16538
                     /gene="flhA"
                     /locus_tag="KR440_000639"
     CDS             14463..16538
                     /gene="flhA"
                     /locus_tag="KR440_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HBH0697705.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVSKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAEIKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDDDKTIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMNNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            16513..17529
                     /gene="flhF"
                     /locus_tag="KR440_000640"
     CDS             16513..17529
                     /gene="flhF"
                     /locus_tag="KR440_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HBH0697706.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIQGIFS
                     KKDIELTIGWEKRENVEQKDLKREIEQLKLIINNMGFDNKNDNDIDKICKNLLSLELN
                     EEIVESIRIDLQEMKFNGIDTSKNLVEILKKKIKIENQAINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVVNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            17526..18404
                     /locus_tag="KR440_000641"
     CDS             17526..18404
                     /locus_tag="KR440_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HBH0697707.1"
                     /translation="MIDQAQILRKMAMEKRGLDEFIVENENVPKIITIASGKGGVGKS
                     NLATNLSICLTKLNKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHDVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKRSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVSLCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            18418..19119
                     /locus_tag="KR440_000642"
     CDS             18418..19119
                     /locus_tag="KR440_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HBH0697708.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDAEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELNVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            19135..19557
                     /locus_tag="KR440_000643"
     CDS             19135..19557
                     /locus_tag="KR440_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBH0697709.1"
                     /translation="MIQIILIVVSILIIVTIVINLHKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEIRSNNHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELSLIRSCYKI"
     gene            19542..19727
                     /locus_tag="KR440_000644"
     CDS             19542..19727
                     /locus_tag="KR440_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBH0697710.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            19768..20541
                     /gene="flgG"
                     /locus_tag="KR440_000645"
     CDS             19768..20541
                     /gene="flgG"
                     /locus_tag="KR440_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBH0697711.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNDYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            20562..21323
                     /gene="flgG"
                     /locus_tag="KR440_000646"
     CDS             20562..21323
                     /gene="flgG"
                     /locus_tag="KR440_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBH0697712.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGNDISTGTGAKATAPMRIYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNIDGLGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            21346..22227
                     /locus_tag="KR440_000647"
     CDS             21346..22227
                     /locus_tag="KR440_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBH0697713.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMIVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVIKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNEYIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTKKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            22230..22604
                     /locus_tag="KR440_000648"
     CDS             22230..22604
                     /locus_tag="KR440_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBH0697714.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELNTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            22636..24038
                     /locus_tag="KR440_000649"
                     /pseudo
     CDS             22636..24038
                     /locus_tag="KR440_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            24154..26091
                     /gene="htpG"
                     /locus_tag="KR440_000650"
     CDS             24154..26091
                     /gene="htpG"
                     /locus_tag="KR440_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBH0697715.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITIQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
     gene            26479..27609
                     /locus_tag="KR440_000651"
     CDS             26479..27609
                     /locus_tag="KR440_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBH0697716.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDIDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
     gene            27836..28858
                     /gene="splB"
                     /locus_tag="KR440_000652"
     CDS             27836..28858
                     /gene="splB"
                     /locus_tag="KR440_000652"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HBH0697717.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
     gene            29040..29951
                     /locus_tag="KR440_000653"
     CDS             29040..29951
                     /locus_tag="KR440_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH0697718.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYNTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            29966..30382
                     /locus_tag="KR440_000654"
     CDS             29966..30382
                     /locus_tag="KR440_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBH0697719.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(30476..30868)
                     /locus_tag="KR440_000655"
     CDS             complement(30476..30868)
                     /locus_tag="KR440_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0697720.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            31009..31404
                     /locus_tag="KR440_000656"
     CDS             31009..31404
                     /locus_tag="KR440_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBH0697721.1"
                     /translation="MKALEFLNKAGVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
BASE COUNT        13006 a         3373 c         5857 g         9512 t
ORIGIN      
        1 gagtgagtgt gaatacatat gacattataa aaatgggatt agatgcaact aatttaagaa
       61 gtcagacaat agcaaataat atagccaata taaatactga aggatataag agacattatg
      121 taacttttga agaaacatta aatgatgcta tgccaaaaat agaagaaaaa acagacaatt
      181 ctaaaggatt aagagtggat ggcaataatg tagatataga agaagaaaaa gtaaatcaag
      241 caatggcaac attacaatat aatgctttga taagttttgc aaatggaaaa atagcaatga
      301 ctaaatcaat aataagtggg aggtaattaa aaggtgagtg tgttcagtgg aatgagaata
      361 tctgctagtg gattaacagc agaaagcttg aaaatggata tcatatcgtc caatgtagct
      421 aatataaatg ccacaagagg tccaaatgga gaagcatata ggagaaaagt tgtttcattt
      481 gaagaaaact atgataataa attaggtatg ctaggagtaa aagttacatc tgttgaagat
      541 gataaatcac ctcttaaaga agtatatgac ccatctcatc cagatgcaaa gccaaatgga
      601 tatgtagaat atccaaatgt aaatatattg gtagaaatga cagatttaat aacagcttca
      661 cgagcatatg attctaacgt agatacattg aatgctcaga agtctatgat atcaaaagct
      721 ttggaaatag gaaagtaggg ataaattatg ccaattacac aaattaacaa tttaatgcaa
      781 ggtgcaaagt tagataattt gattggagaa acaaaaccat taactcttaa taataaagaa
      841 gaaaaaaaga actttgaaga tgtaataata gatactatag ataaagttaa ttttcaacaa
      901 gtgacagcag atgatatgaa agaaaaattt ataaaaggcg aagatgtttc tatgcatgaa
      961 gttatgttaa taggtcaaga agcacagcta tctatgcaat atttaataga agttagaaat
     1021 aagattcatg atgcatatca agaaatgaat aaggtacaaa tttagccttt tgaacaggag
     1081 taataatcaa tgatagccag ttttaaaaat aagattaaag agcaagttgc taaatctagg
     1141 acaaagataa gttctctaaa aaagggaact aaaatagcat taggtatagg ggttattgct
     1201 atatgtttag cgctagtatt tacagcaata tctgcgagca aaaataagta tgcagtctta
     1261 ttttcagaac tagatgttca agatgctgca gatatatcag cagaattaga gtctaaaaag
     1321 atagatacta aggtaaaggg aagtacgatt tatgtaccta gaacagaagt tgataagtta
     1381 aggctggagc tagcaccaag tcttacaggt ggttctaaag gatttgcact aatggatgat
     1441 agttcatcaa taggacttac tgatgaagag tttcagatta agaagcaaag aatgatacaa
     1501 ggtgaaatag aaaaaacaat aaagagtttt ccacaagtgg aagatactag agtacatata
     1561 acatttggag agtcatcagt atttgagaag gaggctattc cagggagtgc agcagtttat
     1621 ttgatgttaa aaccagggac gactttggaa aaaaaccaaa taaaatctgt tatggcactt
     1681 atatcaggaa gtgttacaaa tataccagag aaaaatgttc aagtcattga taacaaaatg
     1741 aatttattat ctgaaggtat tttagaggat gaagatgttg atgaaaataa tcaaccaaca
     1801 aatagtttga ttaatgttga taaaaataag gcatctgaac aagcattgaa taaacaatta
     1861 gaaaagtcta ttttagaaat gctagaacca atatttggta aaggaaaagt aaaagccaca
     1921 gtaaatgctg atttaagttt tgatacagct gaaaaaactg agataaaaat tgaccctgat
     1981 aaagttgcaa taaaggagac aaggtcgaaa aattcatcta aatctgctac aaataatgtt
     2041 caaggtgtag atgctaacat gaataaccaa ggaaataaca atggtactaa tacagaagat
     2101 agtttagatg agagttttga gtatgaaaca ggtaagattg aaactcacac tatagttgcg
     2161 cctggagaac ttaatagatt gacagcttct gttgcaatag atggaaaaat agctaaaggt
     2221 gtacaagctg atgtagagga tattgttaat aatgctatag gtatggatgg agcgagggga
     2281 gatactctta gcgttgtttc aatggtattt gacccagaag ggaaggcaga agctaaacaa
     2341 gagctagaag agattaaaaa agaaggattt aaaaagaaca taataaaagc agttattgga
     2401 gtagtggcag tggtagcaat tggagctatt ggatacttaa tatataagaa aagaagtgaa
     2461 cgagaagata tggattatga agatgaagat tatatttcta aatctgattc tgttgatagg
     2521 aaggataatc ctctattagg agatgaccca gaactgacat tagaggaagc tgttaaaatg
     2581 tatgcagaag aaaagccaga ccaagtaaca gagataataa aaacttggtt aaatgagtaa
     2641 gggggataga aagtgtccac ggaaattaca aatgttaatg cgtttgactt aagtgatata
     2701 aatgaaagtg ataagaaaga gaagatagaa ggtgagggtt taaaaaaagc tgctgtatta
     2761 ttaatgacat taggaccaaa tgtgtctagt gatataataa aaagcttgcc tgataaacaa
     2821 gttcaaaaaa taggtttaga aattgcaaat atttcttcaa tagcttcaaa agaaagaaga
     2881 gaaatcttaa aagagtttgt agagataaat agggcaaaag attatgttat agaaggtggg
     2941 cttgaatatg caagagagtt gttaagcaat gctttaggaa atgctaaagc aaacaaatta
     3001 cttgaaggta tatcaataaa tacaagtaca aagcctttta aacatattat aaaactagat
     3061 gtaaagaaaa tattagaaat acttagggat gaaagtgcac aaactatagc tgttttactt
     3121 gcacatatgg agccagaaaa tggagcagaa gttttatcat acttagattt tgatttacaa
     3181 aatgaagtag ctatgagaat aagctcaatg ggttcaatat ctccagaagt aataaaggta
     3241 attgaaagtt cattagaaga taaattacac tcaataagtg gtgatgattt taaggattat
     3301 ggaggagtgt ctaccttagt tcaaatattg attaattctg atagaaaaac agaaaagaat
     3361 attttgggaa ctattgaaga acaagacccg gctttagcag ctcaaattaa atctcatata
     3421 tttgtctttg aagatattgt caagttagat gatataagta tacaaaaagt attaaaagaa
     3481 gtgactatga aagatttagt atatgcctta aagggtgtgg atgatgagat ttcagatgct
     3541 atatatagaa atcaatcttc tagagcagca gatgcactta aagaagaaat ggaaatggtt
     3601 ggtgtcataa aagttacaca ggttgaggct gctcaacaaa agatagcaaa tatagtaaga
     3661 aaacttgaaa gagaaggtgc cataactatt gtaaggaatt ttggtggtga ctttgttgaa
     3721 taataagatt attaaaagta aaaaagcatc atataaagga gtgctagatt taaccataat
     3781 agataaagtt agagtgggag aatcaaatgt aaataaagaa aatgattgta gatttagaga
     3841 tgaggtaata aaagaagcta tagaatctgc tgagataaaa aagaatgaga tattagaaga
     3901 agcaaaaatg aatgcacaaa atatagaaaa acaagcatat gaaaaagggt atagtcaagg
     3961 tcaaaaaaat ggatatgaag atggatataa agagtcatat gatgaatata cccaaaaagc
     4021 taaagatgaa gcagatttta taaaaaatca ggcagactta atactattac aagctaatga
     4081 gaagatgact gagtacataa aagaaaaaaa taaagagatt gtaagacttg ctattaatat
     4141 ggctagttct gttctaaaaa agcatttcga agatgaagaa tctatgagtg atttgttaaa
     4201 gcaaattatt caagattatg aaggaagtag ttcatttata gtaagatgta attctttcta
     4261 taaagataat atagagaaag agtttgaatt attcaaagag gaaacatcac taaaatcaag
     4321 tgtatttgtt attgcagatg atagtataga taaaggcaat gctatcatcc aaaaggaaaa
     4381 tggaagaata atagttggta tcgattgtgc tatggaaaaa ctaaaagaag aattgcttgg
     4441 tgaataaagt atgtataaaa ctgatatatc tatagatttt gacaaaataa ataagaaaat
     4501 agaaaattta gaactcacta tatcagaagg tagagtaaag aagataatag gacttactgt
     4561 agaagttgaa ggaataaaag cttttgttgg ggagttatgt gttatataca atcaagtcaa
     4621 taaaccagtg aattgtgaag tagtgggttt taaagataaa gaagtcatat taatggcgtt
     4681 gggagagcta acattgatag ctcctggttg taaagttatt tctaagggaa tacctttaag
     4741 tgttatgtgt agcgataatt tactaggtaa agtacttgat ggtcttggaa atcctataga
     4801 caactcagat gctgttttag gagatagata caacttgaac aatgaacctc ctgacccaat
     4861 gaagagaaaa aagatacgaa atataatgga aacaggtgta agagcaatag atgcatttac
     4921 tacttgtggt gaaggacaga gaatagggat atttgcagga agtggagtag gtaagagtac
     4981 gactctaggt atgattgcta ggaatgcaaa agcagatgta aatgtaattg cccttattgg
     5041 agaaagagga agagaggtac tagattttat tgataaagat ttaggtgaag agggaatgaa
     5101 aaaatctgtt gttgtatgtg caacatcaga taaagctcct ttggttaggc taaaaggtgc
     5161 tcttactgct actgcaatag cagagtattt tagagaccag ggaaaaaaag taatacttat
     5221 gatggattca gtaactagat ttgctatggc acaaagagaa gttggtatag caataggaga
     5281 accaccagct caaaaaggat atacaccatc tgtatttgct attttaccaa aacttatgga
     5341 gagaacaggg acatcagata aaggttctat aactgcattt tatacagtat tagttgatgg
     5401 agatgacttt aatgagccaa tagcagatac aacaagaggg atacttgatg gtcatatagt
     5461 tttatctaga gacttggcaa ataagaatca ttatccttct atagatgtat tgaacagttt
     5521 aagcagactt atgaatgaga ttgcttcaaa agaagacata aaaatagcat cttttgcgag
     5581 agatatgctt gctgagtata gagaagcaga agatttgata aatataggtg catatgcttc
     5641 aggtacaaac aaaaaaatag atgaagcaat ctactatcat gagcatatta ttaatttttt
     5701 aaaacaagga ataaatgaaa agagttcttt taatgaaact ataagtagtt taagaagaat
     5761 ctttgaataa acaatttaaa aaaatatttg aataggttgt gatggaattg gataaaaatt
     5821 ttaagtttag attacagaaa gttttagact taaaaataaa agatgaagaa gagataaaaa
     5881 tggaatttgc taagattcaa caaaaaaaaa tagatataga gagcaattta gaaaacttag
     5941 aaagtaatta cagcaaatat tctataagta aaaataatga tagtgttcaa aatcaaaaaa
     6001 taactattaa ctatttgtta gctttaaaca actctataat ggatttatca gaagagctgg
     6061 ataagtcgac aaatgaactt gaaaaagcta gaaagcagct tataagcaaa cagatagaga
     6121 gaaagtcatt agaaaaatta aaagaaaaaa aatatggaca gtattataaa gaagaacagt
     6181 taaaagaaca aagcactaat gatgagtttg caagcatgag ttatttgaga aatagacaag
     6241 ttctataaaa tagtaggaag tgaggatatt attgtatgaa gatacaagca gattcttata
     6301 atattattaa agatattggg aatagaaaaa gctcatctaa gaataaaaat ataaaaaata
     6361 ataaatttga agataatcta tataaggcaa cagaccataa aaaggttaaa gatagaagag
     6421 aaacaagtaa gcaaaatgtt agttcagata aacgggaaaa tagtgataat gagtgtcatt
     6481 tagatgaaga taaaagtaat cacttagaag atgaaaatac atatgaaaaa gattgtagta
     6541 ataataatga agctttacat caattactga atcttttaaa agataattac aaaaaagata
     6601 aagatataaa cactatattg aataatctaa aaaaactaag tatatcagat gaattaaaac
     6661 aagaacttaa gcttaataaa aatatattaa ataagattgc aaatcaaaat ttagagttaa
     6721 atagagctag tacaaataac cctaatgata tagaaaattt agacataatg aagttaagag
     6781 aacttttaga aaataatgct agtgaagata ttgaagtaat tagtaaaact acagaaaatg
     6841 atactaaaag catagacttg tacagttttg acagcaatag aatagatact cttaactcaa
     6901 ataaacagaa agatgatagt tctgatattt tggaaaaact agcaggggta aatggaaata
     6961 agagtggaaa ttttggacaa gtaataaata aaagcgttga tatgaataaa attaaaaatg
     7021 ataatgttaa tttaaaacag atagaatcaa atgtaatgga tgattctata aaagctatta
     7081 agcatatgaa aacaaatgat atacaagaat tgacaattaa attaagacca aaagaattag
     7141 gagatatgaa catacaattg ttaaaagatg gtgaaagtat gagagctgtt gtgactgttt
     7201 tcaacaaaga tgtctttgac tctataaata aaaacatagc agatttaaag caacatcttg
     7261 aacttacaaa tgtaaatata aaagatgtat cagtgcaaat gcatagcgat aatacaaata
     7321 catctgatac ctttgataaa gcatttgaac aacagaatag acaaaataac caagaaaaca
     7381 tgagtaagca aaatacaaat gataagcgta acaatgtagt catagaagat gagattaaag
     7441 aaaatattat tgatgatgat agagtagatt tactagctta gtaaggggga agtattgcat
     7501 gtacggaata gcaacggctt tggcaggaac tcaggtaaac tataggtcaa ataatttaag
     7561 ccaacttgaa agaaatatta atgatattgc caataaacaa gttagaagta aaggaaatca
     7621 gacagtaaca gacaatggga ctccaattat aggacaaggc gatgatgctg ataaagattt
     7681 gtttatgaag attcttgtag cacagatggg taatcaagac ccaatgaatc cacaagaccc
     7741 aacagaatat gtatcacaat tagcacagtt tgcaacttta gaaaagatgt cagctatgaa
     7801 tgataagcta gatatacttt tagccttaag caattccact ctaataaatt ctgctttgag
     7861 tatggcaaca tcattaattg gtaaagaagt agaagtatct ggggaagagg aagaagatga
     7921 aaacattgta ggagtagtaa aatccacata tataaaagat ggggaagtac acttagaatt
     7981 agaagtagat ggaagtgaca aaactgtagg tgttaaatat gaaaaattac ttaaaatagg
     8041 agaggtagat gcaaattcag gagaagaaaa tgactcttca gaacaagaat ctccagaaga
     8101 agtataatat taaatgagaa aagaggtaaa tttatgataa aagcaatgta ttcaggtgta
     8161 agtggaatga aagcaaatca aacaaaacta gatgtaatag gtaacaatgt agccaacgct
     8221 ggaactactt cttttaagaa aagttcagct agaataacag attcatttta tcaaactgta
     8281 ctatatgcaa gtgctccaac agcggcatta gggggaacaa acttaggaca agtaggtgta
     8341 ggtagtaaga tatcaagtat aaataaagat atgacacaag gaaatgtaca accaactaat
     8401 aggccatctg atttaatgat agatggagat ggatatcttc cagtagtaag acaaggaaca
     8461 gttatgtata cgagagatgg ttcttttaat ttagatacag gatttaaagc agctgatggt
     8521 gctttgaaga atattaaagg tggaagactt gtaaatggtg atggatatat gttacaaggt
     8581 gtgatttatg atggggaata taaagctgat actggagaat tcattaaaaa gggtgatgct
     8641 aaaggaaaag agcctattgt aataccactt caaagtataa gtcctaagga tggaacagtt
     8701 caaaatgtac tagagtataa tatatcaaag gatggaacag tagaattttt attgagtgat
     8761 ggtcaaagaa ctcaatttgt ctatgatata cctgatggtg ctaaggtagg agatacacct
     8821 gctggaatag ataaattaaa aggtacaata caaaaagtgc aaacatatgc attccaaaat
     8881 ccagcaggac tagatgcaga aggaggaaac ttattcaagc catcagcaaa ctcaggagat
     8941 ccacaaatag caggagctac aggaaacata atacaagggg caatagaagc ttccaatgta
     9001 gatatagcag aagaatttac agaaatgata atagcaagta ggtctttcca agcaaacagt
     9061 aaaataatat cgacatcaga cgaaatactt caagaaataa taaatcttaa gagatagtag
     9121 gtacatatta tgataagagt aacggattta aacggaagaa gatacatatt aaattcagac
     9181 cttatagtga gaattgactc tgtaccagaa agtaggattc tccttacaac aggagaaaaa
     9241 aatcttgtaa aagagtcagt agatgagata gtagataaag taataaagta taaaagaaaa
     9301 gtaaatgtag gttatattag gagtgaaaaa taatgaaaaa aacagattca ctaacattca
     9361 ttggactaat attatctaca gtattagttt tatttggggc agtaaaagga agtagttcag
     9421 gattgaaaag ttttttcgat tttgcatcta tattaatcac agtatttggg tcatttggag
     9481 ctttgatgat tacatttact attgatgata taaaacttat aaaagatgcg ctacaatact
     9541 cattcaagac aatgaatata tcgaaattag acttattaaa tcaatttaaa acactttcta
     9601 aaaaagcaag aagagaagga cttttatcta tagaaagtga tgtaatgaaa atacaagacc
     9661 catttatgaa aaaagggtta gagttatgta ttgatggcct tgaagtaaat gaaataaggt
     9721 ctatactaga actcgaaata gattctgttg aagaaaccaa taacagatat tctaaactat
     9781 ttaaaacttg ggggactttc gcacctgcat ttggtatgtt gggaactcta ataggtttaa
     9841 tacaaatgtt atctgacctt acatctccag atttaatagc atcgggtatg ggaaaagcat
     9901 taattacgac attttatggt tctttacttg ctaatatcgc tttgaaccca attgcatata
     9961 atatagatga gaagactgaa aaagagattt atgtaaagga aatgatgctg gaaggtataa
    10021 taagcataca atctggggag agcagcatag tagttgagga acgtttagct acataccttt
    10081 caaacaatga gaagctagaa attatgaaat ccaataaaaa tacagaaaga gcaatgagta
    10141 atggcgcgta aaaaaaataa gaaaaaggat gagataaatc cagatgcatg gttggctact
    10201 tatgcagata ctataacact gattcttaca ttttttgtgt tattgtatgc tacatcagtt
    10261 gtagatatgg aaaaatttaa aaacatagca gatgcgttaa aaggtcagtt ttctgggata
    10321 agtatgttca gtacaggttc aagtacaagt gagaagtctc caatagagag tgttttagtg
    10381 gataaaaatg ataagtcaaa tttagaacag gatttaaaag ataaagttaa taattctaac
    10441 ttacaaaata gtgtaacagt caaagatgat agtagaggga ttttactaga actagatgat
    10501 tcaattttat ttgactctgg gatttctgaa ttaaaagcag atagtaaagc cactcttaat
    10561 aaagtatatg acatgataaa gatgatgaaa aataagatag ttatagaagg ccatactgat
    10621 aatgtacctg taaaatcaag tacacatgaa tccaattggg atttatcttc tagtagagca
    10681 gtaagtgttg taagatactt tgtagaagaa aagggcatgg accctagact attttcggca
    10741 acaggatgtg gagagtatca acctttggtt tcaaatgaaa cagcaaaggg aagggctgag
    10801 aatagaagag ttaatatact tataactatt gactaggagg aagaggtttg ttcaaaaata
    10861 aaaaaattat tataattatt ttgatactga ttattttctc agcaggagca ggttttttta
    10921 tattaggaga aggtggaaat aaacaaattg acaagattgc aggtttgttt attccaacag
    10981 ggaaaggcaa agatgaagta aagaccgaaa atcttgactt agaagagatg gtattaaagc
    11041 tagctgatga aggtgtaaga tatttaaaag tatcaataac tgtatcttat gattcttcat
    11101 ataaagatat agaaaaaaat acgcctttaa ttagggataa tattataaat tgttttatgg
    11161 caaaaagggc agatgatttt actgctgata atttaaagaa cataaaagaa gaaattaaaa
    11221 atacattaaa tatacaacta ggtgaaaatg taattcaaaa tatatatttc acaaatatag
    11281 tagtgcaata aaaggatggt aagacatgat actttattta ctaaaaatat taatatgtat
    11341 cccaataatt ttaatattga tagtcttatc tttaaaaatt agccaaggaa ctttattaaa
    11401 aaaatataat gataaatata taaaaatact agatattgta aatataagta aaaacaatag
    11461 cataataatt ttaaaaatag gtgaagaagg atgcattatg gcatcttctt cctctagcat
    11521 gaataaaata aaagatttga ccaaagaaga aatattaaga atagaaaacg acattaaaga
    11581 aagaaatttt aaaattcaag attttaaaat ggaaagaatt aaaaagaaat atgatttcag
    11641 tagatatatt ggaaaattta caaaaaagga agataagtaa atggaactat tatcaaaatt
    11701 acctttggct gatgtaccat atacaagttc aatgcaattg tttttatttc taactgcatt
    11761 aatgttttta ccatttgtaa tgtttatgat gacaagcttt gtcagaatag ttataagttt
    11821 atcatttttg aaatcagcat taggagcaca gcaagcaatt ccaagccaaa ttttggtagg
    11881 gcttgcaata gtattgacaa tatttataat gagaccagta ttaaatgaga taaacgaaaa
    11941 ggcattacaa ccatatatga aagaagaagt tactatggaa gaagctatga aggaagcaga
    12001 gggacctatt aaagaatttt tattaaagca aaccagacag acagatttag atttatttgt
    12061 agaacaggct ggtttgaaag aaaagaaatt aactcgtgaa aatataccac tatctgttgt
    12121 agttccagca tttgctataa gtgaactaaa aactgctttt caaataggtt ttttaatata
    12181 tataccattt ttgataatag accttgttgt ggcaagtgtt ttgatgtcta tgggtatgtt
    12241 tatgttgcct ccagtcatga tatcattacc gtttaagtta ttattatttg taatggtgga
    12301 tggatggaac ttgatagtaa aaacattgat attgggattt ggataggaga aaaatagtat
    12361 gagtgaattt atgataatga gtgttcttaa aaatggcatg tttacaggag ctatgattgc
    12421 agctccagta ctcattgttt ctcttgtagt tgggctttta ataagtgtgt ttcaagctac
    12481 aactcaaatc caagagcaaa ctttgacttt tgtaccaaaa ctaatagcaa ttcttttagt
    12541 agtagctatt ggtggaagct ggatgttaca tactcttatt agctttactt cggagatatt
    12601 caatatgatg tctcttataa ctaaataagt aggatatata tgatttttgt atttattaga
    12661 atagtggcat tctttggaac actaaggata ctatttccag ctggtacacc tgctttattt
    12721 aagtcgctct ttgcaatcat tatatcaata ttgatttcaa gtactatgca aattgaatat
    12781 gcaacaaata tagacaacat tgtattgttt accttatatg gtataaatga gaccataact
    12841 ggcattatgc ttggatatat aaccaatcta tgtttttata gtatcagaat ggcaggttct
    12901 atgatggatc aacagatggg attatcaatg attaatatgt ttgacccaaa ttcaatgact
    12961 cagacaactt taattgataa tcttatgaac tggacagcac gtatgatatt ttttagcatg
    13021 gatggtcatc atgttttaat tagaggcata aggtatagtt ttgagttaat tccaataggg
    13081 aaatcattcg tagataataa tatagattat ataataaaca tatttgtgca gtgcttttta
    13141 actggattca aaatagcaat acctattgta ctttgtctgc ttatggcaga ttttatatta
    13201 gggcttatat caagaagtgt acctcaacta aatgtaatga taattggtat gccattaaaa
    13261 atactggttg gtattgcgtt atttatcata tcaatacctt taattgtcaa tcaaataagt
    13321 catttattaa gtcaaatacc aaagatgtat gagggaactt tcgcactagc tcctatgttt
    13381 tttatgggtt caacagataa aacagaagag gctactccaa agaaaaaggg agaacaaaga
    13441 aaaaaaggta atatagcaaa gagtaaagaa ttacccgttg caatgactct acttgcattc
    13501 actttattag tacctacatt attttcatat gtagtggata cacttaaatc ttctcttaat
    13561 tattttttga gtctagattt ttatatgaat ataaattatt caagtataga gaaattagtt
    13621 atagcaggat taatggattt ttttaagata tttttaccaa tagctattcc attcttagtt
    13681 ttaggtatag tcgcaaacct atttcaagtg ggaatactgt ttactggtga aactttaaaa
    13741 cccaatttat caaaattaaa tccagttagt ggatttaaaa acatgttttc tatgagaagt
    13801 ttgtcaacct taataaagga tacagcgatt atatctattt tggcttacat aggatataca
    13861 ttcttccaag ataattattt agatatatta aagctaggta acatatacct gccaacttta
    13921 atgtacacag ttaaagattt agtatattca atattaagta agatttgtgt agctatgata
    13981 gttatagcag ttgcagatta tgtttatcaa agatacagtc ataaaaagca acttagaatg
    14041 acaaaacaag aagttaaaga tgaatataaa aactcagaag gagacccaga aataaaagct
    14101 aagataaaac aaaaacaaag acaaatttcg tctcaaagaa ccatgcaagc agtaccaagt
    14161 gctactgtaa tagtaaccaa tcccacccac ttatctatag cagtgagata tgaaaaaggg
    14221 aaagaccaag ctccagtagt ggtggcaaaa ggtgcagatt atttagcttt taaaataagg
    14281 gaaattgcaa agggaaatga catacctata atagaaaata aacctattgc taggttgtta
    14341 tataagcaag tagaaataga ccaagaaatt ccagaggaca tgtatcaagc ttttgcagag
    14401 atattagtag cagtatataa aatcaaaaat agatacaaag tcctcaaaag gtaggataag
    14461 taatgaataa gtttaattta aaggaaaata tggacgtaat agtggcattt ggtgttgtag
    14521 ggattgttct tatgatgata gtgccgttac ctacatttat attagatatt ttactggcaa
    14581 ttaacataag tttatctgtg ctaattttac taatgacatt atttacaaca aatgtacttg
    14641 atttatctat atttccaaca gtgttacttg tgacaacact atttagattg ggattaaatc
    14701 tttcatcaac tagattgatt ttaacacagg gatatgcagg tgaagttata gaggcatttg
    14761 gaagttttgt tgtaggtgga aattacgtag taggaataat tatattctta attatattaa
    14821 ttgttcaatt tgttgttata accaatggtt ctggaagagt atctgaagta acagctagat
    14881 ttacgttaga tgccatgcca ggtaagcaga tgagtataga tgcagattta aatgcaggag
    14941 ttatagatga caaaactgct agaaaaagaa gaaaagagct tcaacaagaa gcagattttt
    15001 atggctctat ggatggagca aataagtttg taaagggaga tgccatagca ggtcttatag
    15061 taacagcaat aaacataata ggtggaattg taataggagc tgtaatgctg aatatgactc
    15121 ttatgggtgc agcacagaca tatgtaagac ttactatagg ggatggtctt gtaagccaga
    15181 tacctgctct attaatatct acatcagcgg gtataatcgt aacaagagct tctactgatg
    15241 aaaactttgg tggattagta agtaaacagt taacggcaat accaaaagca gtaatgatga
    15301 ctggttgtgt tctaatcgta cttggtatga tgcctggatt acctaaacca gcattttttt
    15361 cactaggtat aggtgctata gcagcaggtt actttttagg aagagaaaag aaagatactt
    15421 tagaagactt ggagtttgaa aaacctgata tggtaaatcc agataaaaat gaagtagaag
    15481 atgtaacaaa cttaataaat gtagaatcaa tagaagtaga aattggatat ggtttaatat
    15541 ctttagctga tgaatcagca ggaggagatt tacttcagag aatagcatca ataagaagac
    15601 aatgtgcaat agacatgggt atagtcgtgc aacccataag aataagagat aatttacagt
    15661 taaatccaaa ccaatacaat ataaaaataa agggaaatgt agttggtggg tatgagataa
    15721 tgcctaatat ggttttatgt atggacccta tgaatcaagg atttagtata tttggaataa
    15781 agacaattga gcctactttt ggaatagagg cactttggat agaaagtgac aaggtagaag
    15841 atgcagagat aaagggttat acagttgtag attcatccac aatacttata actcacttac
    15901 tagatataat aaaatcaaat gcacacgaat tactgggaag acaagaagtt aagactatta
    15961 tagatgcagc aagagaaaat tatagtgctg tagtagatga attgatacca gatttaatga
    16021 cacttggtga aattcaaaaa gtacttaaaa atctgttaag agaaaaagta aatataaagg
    16081 atagggttac aatacttgaa acgcttgcag accagtctag aaatacaaaa gatatagagc
    16141 ttcttactga atatgtaaga atagccttgg ctagaagtat ttgtaataat ttagttgatg
    16201 atgataagac aattgtagtg gctacattgt ctccagaaac tgaagagttg gtatctaata
    16261 atttacaaag gtctgtaaat ggaacttatc cagcagttga cccagaaaat acaaataaaa
    16321 tatttgagag tatacaagaa gtaatgaaca atgtatattt caatcataat ataccagtaa
    16381 tagtagtatc accaaagata agagctccat ttagaaaatt agttgaaatt gtatatccta
    16441 atttgactgt attatcatta aatgaaatac ctaacgatat taaaatcaaa gcagaaggtg
    16501 tggtgagcat atatgatgac gaaaaagtat acagctaaca caattcagga tgctatgaat
    16561 ctagccaaaa tggaattggg tgacaatatt acattaatag ataaaaaaga agtaagaaag
    16621 tctggaatac aaggtatctt ttcaaagaaa gatattgaac taactattgg atgggaaaag
    16681 agagaaaacg tagaacaaaa agatttaaaa cgagagatag aacaattaaa gttaattatt
    16741 aacaatatgg ggtttgataa taagaatgat aatgatatag ataaaatatg taaaaatcta
    16801 ctaagtttag aattaaatga agaaattgta gaatctataa gaattgattt gcaagaaatg
    16861 aagttcaatg gaattgatac aagcaaaaat ttagtagaaa ttttaaagaa aaagattaaa
    16921 atagaaaatc aagcaataaa tggtaaaatt gctctagtcg gaccaccagg agtaggtaaa
    16981 acgactacaa tagcaaagtt agccgcaaaa ttagtttttg aagaaaataa aaaagtagga
    17041 gtaataacta tagatacata caggattggt gctgtagaac agttgaagat atatactgat
    17101 ataatgaata ttccttttaa aggggtaata agtcccgatg aaatggaatt ggcacttgat
    17161 gagatgaaag attgtgatgt tgtattaata gatacaacag gtagaggcta taaaaactct
    17221 atgcaaatac tagaaatcaa aaatcttata gataaagctg aaactgataa tatacattta
    17281 gttgtgaatt gtacaacaag agaaagtgat acaaaagcaa ttatagattc atatagaaat
    17341 gtgaatttta aaagcttaat aattacaaag ctagatgaaa caataacata tggctcaata
    17401 tttaacataa tgaattatgc acaaaaacca atatcatata ttacaacagg acaaaatgtt
    17461 cctgatgata ttataaaacc taatgaagat aagattataa ggttactatt aggagttgaa
    17521 agtatatgat agaccaagct caaattttga gaaaaatggc aatggaaaaa agaggacttg
    17581 atgagtttat agtagagaat gagaatgtac ctaagataat tacaatagct tctggtaaag
    17641 gcggtgttgg aaagagtaat ctcgctacaa atttgtctat atgcttgacc aaacttaata
    17701 aaaaagttct tatattagat gcagacatag gaatgtctaa tatagatata ataatggggg
    17761 taaatgtaaa agggactata attgatgtaa ttaatggcga aaaaaatata gaagatataa
    17821 tttcacagac taagtatggt gtaaatataa tatctggagg ttctgccctt aatcatatag
    17881 aagactttac agaagctcaa agaaataagt ttatacatag catagaacag attcacgatg
    17941 tggattttat aataattgat acaggagctg gtatgagcaa aagcctattg tcatttatat
    18001 actgtagtac tgaatttttc ttaataacaa ctccagaacc gacatcattg actgacgcat
    18061 atagtttatt aaaagctata agtaattttg gcataaagag aagtgcaaat ataataatca
    18121 atagggtaat tgatttagaa gaagcaaaat ctacatacaa aagaataaaa atggttgtgg
    18181 acaaatttct aaaccttaat ctagaattgt atggatatat aatagatgac aaaaaagtaa
    18241 gtttatgtgt aaaaaaacag ataccattca ttttagaata tcctacaagt attgcatcaa
    18301 aatgtattat agccatagca aagagaatgg taagtcagtc aagagaagat attgttgata
    18361 ataaaagttt tctaagaaag atgtttagta tatttaaaag ctaaggaggc gtagttaatg
    18421 aatagagaag aattaataaa agagaatatg cctcttgtaa agagtatagc aagaaaattt
    18481 tttataccag ggaaaggttt tgaatatcaa gacttagtaa atagtggagt cattggattg
    18541 attgatgcaa taaataaatt tgatgctgaa aagggggcaa agttttctag ttactcttat
    18601 ataaaaataa aatctgctat attagatgaa attagaaatc aaagtccaat ttctaaacat
    18661 aatctaacta aagtaaataa gtataataga gtggtagaaa agttgcaatc taaattatta
    18721 agagagccaa cttcatatga aattgcaaaa gaattaaatg tttcagaaaa agaacttcat
    18781 gatatcgaaa gtaatataga catgttaaat attgtatcat taaactatgt aatctttgaa
    18841 gatacaaatg aaactgtaca agatgtaata agtgatagag aagaggaagc tccagaaaat
    18901 ataatagaag aagaagaaaa gttagagatt ctatctaaag caattagtaa tttaaatgaa
    18961 agagaaaaat taatactttc actgtactat tatgaagatt taaatctaaa ggagataggg
    19021 aaagtgctag gtgtttcaga atcaagagtt tcacaactcc acagaaaatc aataagaaac
    19081 ttaagaaaca agataaaaga acttaaatat tctatataga tggtgattga taagatgata
    19141 caaattatat taatagtagt gtctatttta ataatagtaa ctatagttat aaatttacat
    19201 aaggaatcaa aaaataaaga taacagatac tttgataaaa tatttgagca agaatatgaa
    19261 gcaaatgata gattaataat tgcagaaaaa agaagattct ttttagacaa agtacttgct
    19321 gaaataagaa gtaataatca tataaaagtt tatgaaaatt ataacctaat aaaggctaat
    19381 gaagaaagaa aggaaaacat atttatagaa tcaaaaaagg aagtaagaca agaaaatgag
    19441 ttatctctta aagtgaaaca tcttataagt tcaggttatg atgatgtgga aatatgtaag
    19501 atgctagata ttggaaaggg ggaactgtca ttaataagga gttgttacaa aatataagga
    19561 tattattatg tgataaaaaa gtattcttag gaataggtat tggtatatta atatcaactt
    19621 tatgcataat gctttttagt aataatgata tgagtaaggc agagatagaa aaaaaggcca
    19681 gagcgttagg catgaaatat acaagtgaga tgaaagtttt agaatagatg atactgttta
    19741 gacatttaca aaaaaggagg tcataatgtg ataagaggat tatatacagc agtttctgct
    19801 atgataacta atcaacaaaa gcaaaatgtt gttgtcaata acctagctaa tatggataca
    19861 aatggatata aaagtaaaca acttttgact aaaagttttg atgaattaaa gttggagggt
    19921 tacaataact acgcaaatgg aactaaacaa aagcagatta taggcgatat aagtccaggt
    19981 atttctatgg atgaaactat cacaaatttt aatcaaggac ctataaaaac tactgataat
    20041 aaaatggatg tagctattca aggtaaggga ttttttctag ttagtgatgc agctggaaat
    20101 caattttaca ctagaaatgg gaacttcaga acagacaatc aaggtgacct tataacaagt
    20161 gaagggtatt atgtacttgg gacgaatact gctactggtg ctgtaggtca tataaatgta
    20221 ggaaatcaaa aatttgaagt ggcaaaagat aatacaatat cactagatgg aaatgctatg
    20281 tataaattca atattgtaga ttttaatgac tacaataact taaaaaaaga aggagataac
    20341 ttgtattctg gaggaggagc tatgaaagta aataatgcac ttacaataca aagtgcgata
    20401 gagtcttcta atgtagatat ggtatctgaa attaataata tgatgaccat atcaagagaa
    20461 tatgaggcaa atcaaaagat aattcaggct atggattcta aattagcaaa aatagcaagt
    20521 gagattggtt cagtaagata ggtgatagga ggtgaatttt tatgtacaca atgttttata
    20581 ctagtaaaac agctttatca gcaaatcaaa gtaaactaga tataatatct aacaatatag
    20641 caaattctga ttcaacagga tataaaaaaa taaatatgga atattctgat ttagtggatg
    20701 aagtactaaa tagaccatca tatcctacaa atggcaatga tatatcgact ggaacaggag
    20761 ctaaggcaac agctcccatg agaatttatg aacaaggtgc attggcccct acagatagta
    20821 agagtaattt ggcgatagat ggagaaggat tctttagagt gataagaaaa gatggtacat
    20881 atgcatatac aagaaatggt ggatttaata tagatggttt aggtaagatt gtagatgata
    20941 atggaaatat attagatgtt caatttgatg caggatataa ctataataat acgaatatag
    21001 atagcacaaa cttaacagtt tcaagaaatg gcgagttatt cgtagataat aaaaaaatag
    21061 gaaaaatcaa tttatatcaa cctataggaa ctcaaaattt tatatctgaa ggagatagtt
    21121 tattcgtcgc aactgatgca gcacagatta aacaagttga gaaagtagat atagttcaag
    21181 gctatagaga gaggtcaaat gtttctttac aagaagaatt tgttgactta atagccactc
    21241 aaagagcatt tcaaatgaac tcaaagggaa taaagacagc agatgaaatg tggcaaatag
    21301 ctaataattt aagagctaaa taattatgta aggtgaggaa tattcatgaa tgatatgaaa
    21361 gagtacgatt ttggcaaacc tcagagttat tcaagagaaa atttaacttg tttaaatttt
    21421 ttattagctg aattttgtaa aaaatataaa aactatataa tttatgaact taagtgtaat
    21481 tctagtatga ttgttgatag tatagaccaa gtaaattatc agactttttt agatagagta
    21541 acttcttcat gtgtagtagt acaaaatgca atggaacctg ttatgaaaaa cttttctttt
    21601 agaatagata gaagtgtagc tgatatgtgg attgatataa cttctggagg aactggagtt
    21661 ataaaggata atgatagaga gcttacagaa cttgataaaa aagtgctttt acatcttatg
    21721 gatgatttag taagaaatat gcacttattt gaaggttttg aaaatataca agtattggat
    21781 acacatacac atattaattt accacaatta tgttctccaa ctgcaccagt atgcgttgta
    21841 gatttaaaag tgctaaatga taatgagtat attggaagtg tttctctttg ttttccgtat
    21901 aatggtttag aggctctatt agaaagtata tctgttatag aattttttga taatgatgta
    21961 ggagatgatt cagaggaatt taccaaaaaa atctataata gtgtatctaa tatagaatta
    22021 tcagttattg cagagatagg aaaagtatca attaatgttg aagacttact taaactagaa
    22081 gttggagatg taatagtaac aaataaaaaa ataaatgatt atatagatat atttgtagaa
    22141 aattcaaaat catatacagc aacaccagga tttatatcaa gcaaaaaagg tgtaaaaata
    22201 aatgatgctg ttggaaaaga ggtgtagtta tggacaatag taatcttatt tcagatgaag
    22261 aagcaaagat gttattaaat gataaagttg aaagtgaagt tgatggggat gatgagttag
    22321 ccttagatgt ctctgaagtt aatcgaaata atatacaaag gatacttgac cttaagttag
    22381 aattatctgt agttataggg agaactaaga tgactctaag agatatttta aatttacata
    22441 aaggttctct tattgaactg aatactttag cagaccaaga tgtggaaata ctaatagata
    22501 ataaggtttt ggcttatgga aaagtagtgg tggtagattt aaattttggt gtaaagataa
    22561 caagtatagt aagtgaagaa gacatggtaa aatctcttgt ttagagataa catcaattaa
    22621 tacttaaggc atgaggtggt acttatttta aacttaaatg atataaaaaa agatatacta
    22681 aaactcaata aaaaagactc atgttctcta tataatgaat ctattaataa tataaataat
    22741 aattgtatat caactggatt aaaattttta aaacaagcaa gagatttaaa cccagatgat
    22801 gttgatatat taaatctcac aggtcttgta aacttactaa aatgtaattt tgatgatgct
    22861 gtagaaagtt tctataagag ttcactttgt gaaaaaactg ctctgtgtga aaagtatata
    22921 aacatgctta catctgagga gtttttaatt ttctttgaaa agtacaatca acttataagg
    22981 ttaataaata aaggaatgta taaagaagct atacaagggt ttaaacttat aactgaccaa
    23041 tactgtgatt taatagaacc ttatgagttg cttgccttac tgtatgacaa ggaagaagaa
    23101 tatataaaat ttgatgaatg tttagaagta ttgaagatta ttgataagga aaattattta
    23161 ttaaatcaag aaaataacac attagatatg tataatagtg ctaatataca aactaaaagt
    23221 aatgtaaaag tagatgatta taagaatgag tttttacata aaagtagtag taaccataaa
    23281 attaatgatt ataataatgt aactaaagaa aaaagttcaa gcaaaaacca gaaaagttca
    23341 gattctaata agaaaaaaag tattaaaaaa ataagtacct atatatagcc ataatattag
    23401 gaatattatt agtaggacaa agtatatata gttcttataa gatgaataat ttaactgata
    23461 aaatatctaa aaataatgaa actaaaaatg aagagtcaat agattctaac ttgactaaga
    23521 aagaaaataa tatagaagaa aatgaagata tgagtaaggc ttctaatata aataagagta
    23581 ataaaaacat taataaagag gctgatgtaa aatctaaaaa aaatactgta gaaaatgaag
    23641 atgataaaga ttctgactta tttacagagg atgaattgat gtctaaggcg aatactttaa
    23701 aatcagaaaa aaaaactaaa tcttctatac aactatataa gaaagttgca gatataggtg
    23761 aaaataaagg gaatacatct gaagcaactt atcaagttgc tattctcagt gaaaagttaa
    23821 aagactataa aactgctgaa gaatattata aaatgtatgt agaaaattac tcagaaaaag
    23881 atgcttattt tgatgaatct tattataatt tagggatgat gtactataac aatggtgatt
    23941 taaaaaattc taaattgaca ttaaagaagt tagttaataa ggtaccaaat agtatgtata
    24001 ataattcaaa agtaaaagaa attttaaaag aagaatagtt aaattagata agaaaaataa
    24061 attattttat tttaaaataa tatgattata atatattgac ataccatttt aaaaaattta
    24121 aaatattaat tataaaatga ggagggataa aaaatggaat ttgaaaaagg gagtatttca
    24181 attcatacag aaaatatttt tcctataatt aaaaaatggt tatactctga taaagacata
    24241 tttataagag agcttataag taatggatgt gatgcagtaa gcaaacacaa aagacttgta
    24301 tcactaggag aaatatcaga aaataagtca tcagattata aaattacagt atctgtaaat
    24361 aaaggagaag gtacacttaa atttattgac aatggaattg gtatgactga agaagagata
    24421 aaaaaataca taaatcaagt tgctttttca ggtgctgaag atttctttaa taaatataag
    24481 gataagatgg aagagtcaaa tgacataata ggtcattttg gattaggatt ttattctgca
    24541 tttatggtgt ctaaaaaggt acaaatagat acactttcat atacagaagg agcgactcct
    24601 gtaagatgga tatctgaagg tggtacagaa tacgaaatat ctgaatcaga tgctagaaat
    24661 gatagaggaa ctactataac actatttatt gatgatgata gtaaagaatt tttagatgaa
    24721 tttactgtaa gaggaataat aaataaatac tgttctttct taccagtaga aatatattta
    24781 gaagatgttg aaagattaga aagagaagct aaagaagcag aagaaaaagc taaaaaacaa
    24841 aaggaagatg gaaaagaaga gattgtagat gcaaaagtaa ttgagccttt aaacgacact
    24901 aatcctctat ggttaaaatc tccaaaagac tgtactgatg aggagtataa agagttttat
    24961 agaaaggtat tcaatgtatt tgatgaacct ttattttgga tacatttaaa tgtagattat
    25021 cctttcaatt taaagggtat actttacttc cctaaattaa aaaatgagtt tgaactgact
    25081 gaaggaaaag taaaattata caacaatcaa gtgtttgtgg ctgacaatat aaaagaagtt
    25141 ataccagaat ttttactttt attaaaaggt gttatagact gcccagactt accattaaat
    25201 gtatcaagaa gtttcttaca aaatgacagg gatgtaagca aaatttctaa acatataatt
    25261 aaaaaagtag ctgataagtt aaaatctctt tgtaaaaatg aaagagaaga atacaataag
    25321 ttttgggatg atatacaaat atttattaaa tatggttgct taaaagatga aagtttctat
    25381 gagaaggtaa aagaatgtat attattcaaa actattgatg atgagtatat aacaatacaa
    25441 gattatttag aaaaatgtaa ggacaaacat gaaaataagg tattttatgt aagtgataag
    25501 gaacagcaat ctcaatacat taaactattt aaagaatacg acttaagtgc tgttgtttta
    25561 aactcatcaa tagatactca ttttatttca tttatggaat ataaagagaa tggagttaag
    25621 tttaatagaa tagatgcaga cctttctgat gtattaaaag ataaaaatga aaataaagat
    25681 agtgaagaaa acaaagaaga gatagctaag atagaaggtt tgtttaaaga agctgttgga
    25741 gaaagagtga agaattattc tgtagaagga ttgaaaaatg aagacactcc agctatggta
    25801 ttagtatcag aacaatctat tagaatggca gaaatgcagt caagatttgc tggaatggat
    25861 ttaggaatga attttgagga agaaaaaact cttgtaataa atgagaacag tccaataatc
    25921 aaaaaattgg tttctcttaa agatgatgag gaaaagaaag ataagattac gcttatttgt
    25981 aaccagatag cagatttagc tttactttct aataaagaat taaagccaga tgagttagat
    26041 tcatttgtac aaagaagtaa taagcttatg agtcttttga tagaattata agattgaatt
    26101 tgaatacatt gaagcaaatt tataaaaagt attcaatatc agtgatgaga aagaagtgtc
    26161 tcaaaatgaa cttttcagtt catgaggcgc tttttttatg tgaaattaaa tttattttaa
    26221 tcatttcaac aatgaaaaat atatttatct ctattttgag acagaccgtt ctttattttt
    26281 ttatctttgg ttaattttta aatatttaag taggttctaa taaaaaactc tatttagtta
    26341 aatatagtta acttgatata taataagata atacatataa ttaagtaaaa gattgttttt
    26401 tagtttatgt ttaaatacat aattacactt tgttaaaaac ataaaattat tactaaagta
    26461 gaaataggag gttttagcat gaattttaat tataatttgc cagtaaatat cttatttggg
    26521 agaggcagaa ttaatgatgt gggaaaagaa gtatctaaat atggcaaaaa agtattgata
    26581 gtaacaggta aaaatagtac aaagaaaact ggattacttg ataaaacaat agatttacta
    26641 aaagactcta aaatagaata tgaagttttt gatagagtag aacaaaatcc tcttataact
    26701 actatatatt caggcgtaga aattataaaa tcaactggat gtgattgtgt tttagggctt
    26761 ggtggtggaa gcattatgga tgctgccaag agtatagctt tttcttataa aaacccagga
    26821 gatttaaatg attatatatt tggaattaga caaggtgaag aagctttacc tataatctta
    26881 gttccaacaa catcaggtac aggtagtgaa ggaaattgtt ttgcagtact tacaaattca
    26941 gaaactaaag ataaaaaatc tataaagaag aatagtatgt atgcaaaagc atcaataatt
    27001 gacccagaac ttatggttac aatgccaaag catataattg catcagttgg atttgatgca
    27061 cttgcccata atatggaagc atatctttct aatggaagaa accctcttgc agatgtacaa
    27121 gctatttatg ggattgaact tatatcagaa aacctaataa aggtatataa tgatgttaat
    27181 gatttagaag catgggaaaa aataacactt gcaagtacta ttggaggaat gacgattggt
    27241 acagcgggta cagccttgcc acatggaatg gagcatcctg taagtggtct tagagatgta
    27301 atgcatggaa aaggacttgc agcgttgact cctactatta tagaaagttc atgggatatt
    27361 gatattgaaa gatttggtaa tatttcaaag gtattaggag gaacttgtgc agaagattgt
    27421 tcagatgcaa ttagaaattt ccttaaaaaa atagatttag atataaaact aagtgattta
    27481 ggtattgaaa aatcagatat agattggtta actgataatt gtatgaaaat atcagttgca
    27541 aatattaaga gacatccaaa gtattttaat aaggaacaaa taaaggaaat gtaccataag
    27601 tctttataat ttgactttac atatttagtt ttatataaaa attataaatt aataataaaa
    27661 aattataagt aattaatagt aaaaaaattg tgtatagaag ttatatatat aataaacttg
    27721 tcttaagctt ttattttact ttctaataaa taaaggagtt taaaaaaggg aagattaatt
    27781 aagtaatatt ttataaaatt cttcgattta aaaactttaa gtagaggtga aataaatgtt
    27841 taatcccaaa agaataatat tcgaaaaagg aactttagac actgatgtgg gcaaaaaaat
    27901 atacaataaa tttagagata ataaagatgt agagataatt aatatatcat caaataggat
    27961 taaagaacac attccaggtg aaaatctata tgaacaatat agaagtggga agcagactat
    28021 ggtagttggg attagaaaaa gtctgaagtt tcaaacatgt aaaccttctg ctaattatca
    28081 actaccactt gtcagtggat gtatgggaag atgtgagtat tgttatttga acacacaatt
    28141 aggtgataaa ccatttataa gggtgtttgt aaatgtagat gaaatacttg aacagacaaa
    28201 aaaatatatt gaagatagaa agccacaggt aaccatattt gaaggggcag ctacatcaga
    28261 cccagttcca gtagagccat atactaattc tttgaagaaa acaatagagt tttttggaag
    28321 agaggaaaaa ggaagattta gatttgttac taaatacagt gatgtggata gcttattgga
    28381 tgctgaacat aataatcata cagaaatacg atttagtatc aatactccta gaattataaa
    28441 tgaacatgag cattatacag cctctgcaaa taaaagaata gagtcagctg taaaacttgc
    28501 tagggctggt tatcagattg gtttcataat agcacctgtg tttttatatg aaggttggaa
    28561 agaagagtat aaaaatctga ttataagtat aaaagaagct ttaccaaagg attttgataa
    28621 aaaaattata tttgaagtta tatctcatag atatacaact aaagcaaaaa atagaatact
    28681 agagatttat ccagatacac tattgccaat ggaaaatgag gatagaaagt ttaaatacgg
    28741 tcaatttgga tatggtaagt acgtgtatga taaagaacaa ttagatgata tgaaagagtt
    28801 ctttcttaag gaaataaatg gagtatttaa aaatagttta ataaaatata taatatagca
    28861 aatcttacct aaagatttgc tatattttat ttttatatat tttatgttta taataaagca
    28921 atgagattta gcactatcat ataagaaaat taaaaagggc actatctatc aagaacattt
    28981 tttctagttt tgatactata aagggtaaga gtaacaaaaa tatcaggagg agttatagta
    29041 tgcatgcatg ggagtctatt caaattacat tggatttaat agaagcaaat ttatcagaag
    29101 aaatatcaat taatgaatta gcaaataaag caaatttatc accattttac tatcaaagac
    29161 tatttaaaag attggtaaat aagactgtaa tggagtatat aaagcttcgt cgtcttgcaa
    29221 gagcttctga atacttagca gaacataaga atcgtatact tgatgtagct ttaaattttg
    29281 gatttgctag tcatgagaca tttacacgtt cctttaaaaa agcatatgga ttaactccag
    29341 aaaaatatag agcaaatcca gtaaaactca atcactttat taagccagaa ttaattttaa
    29401 attatgctat ggttgatgaa ggtgttccac tgattgcaga tgatatagtg attgaagtca
    29461 atcgtaagac attgagtaat ccaaggacat ttgttggaat agaaatagaa gtacctattt
    29521 gccagttggt gggtggtgaa acaactggaa ttgcaatagt agaggaatta tggatgaaac
    29581 ttggaagtca aagacataat ataccaaatc aaattccagg tggaaatgaa tttgctgctc
    29641 tttatatggg aaatgcaaaa gaaggaaatt gtatgtatat ggcaggtgta gaggtagagt
    29701 caggtacatc agtagaagga tataatactt ttgagttgcc agctaaagaa tatttagtat
    29761 gtggatttga ggcagaaaac tttaatgagt tagttaattc agctgttttt aaagcagata
    29821 aatttatgga aagatggatg aagaaacata atttaacaac tactgatttt gcaattgaga
    29881 tgtattaccc cactacacct gaagcagcat atctggaaca ttggattgtt ccggttccta
    29941 ttgaacaata gaaaggatga tgaatatgaa ttggcaagat gcgttttttc aagatactaa
    30001 acctacattt gagcaaatta atgaatttat taataatcca ttatgggaca agttaaataa
    30061 aactttggtt tctacttata atgtagaacc taagcttgaa tacagtaaat gttcaatgca
    30121 aagaggttgg aatgtaaaat ataaaaaaag aggtaaatca ctatgtactt tatatccaca
    30181 agaaggttat tttaaagctt tggttattgt aagtgaaagc aatagagtag aggttgattt
    30241 atttattaat acttgttgtg attatatcaa aaaaatatat aatgaggtaa acttttttaa
    30301 tggaagtaaa tggctaatga ttcaagtaga tagtttattg gtattaaacg atatgcttga
    30361 actaattaaa tttagagctt aacatataga acaggatgaa tatataagtc atcctgtttt
    30421 tatattcaat cagataaatt aatatatagt ctataatatg aataatttag taaatttact
    30481 ttatctttct acattctttg caactaagtc cacatgtgtt tttttcttgc atattagtag
    30541 caccccaatt agcaaattgt tgtaaaatag gcacaatact tttaccttta cttgttaatg
    30601 tatactcaac ttttggagga atttcattat attgtttacg atgaacaata ccagtagact
    30661 ctaaatcttt taatgattga gctagcatag tatttgtaat gccatccaat tttcttttta
    30721 tgctactata tctaattgga ttttcaactg ctaacataca tataataggt aatttccact
    30781 taccaccgac aatgtctaat gcataacgga ttggacaggt taattcttca acgtttttag
    30841 ttatattttt ttcagtattt atactcattt taattactcc ttagttatag tagtatcttt
    30901 tttatacttt atcagaaaaa actgcatact tgttttttct gtattttata ttaaaattat
    30961 atcaaaggat aatccttaat acaagtattt aaaaggggtg taagaattat gaaagcatta
    31021 gaatttttga acaaggcagg agtttattat ttagcaacag ttgatgagaa caatcaagca
    31081 catgtgcgtc ctttaggatt tgttatggaa tacaatggta aacttacatt ttgcacatct
    31141 aatcaaaagg atatgtttaa gcaacttatt tttaatccaa aggttgaact ttgttgtata
    31201 gaccaaaacc ttaatactct acgtatttta ggtgaagcag tatttgttac atcaaaagaa
    31261 acacaagcta aggcacttga aataatgcca agtttaggtc agatgtactc tgtgggagat
    31321 ggtaagtttg aaatattctc tattgataag gcaaaagtta gttgttgttc aatgagtggt
    31381 aaaaagattg atgtagagct ttaattataa ttgaatcgac atccatagtg aaaattaaga
    31441 aaacgttatt actgaaatat tgatagattt aatttaagtg caataaaaag atttttaaat
    31501 aatgaatatg taaaaatttt tagttattag atgtgtattt caataatgat atactatttt
    31561 gaataattac atactagaaa taagataatt aaaatttact ttataatggg atagatttct
    31621 tttaaaaaat attttaacta taattgattt ttatttaatc tgaaaaaaat attatgacta
    31681 taaaaaattg ctacttatta tttggatatt tttgtatata aataataagt agcatgactt
    31741 tatacatt
//
LOCUS       DAEPOZ010000017        31725 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.017, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000017 DAEPOZ010000000
VERSION     DAEPOZ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31725
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.017"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            8..214
                     /locus_tag="KR440_000657"
     CDS             8..214
                     /locus_tag="KR440_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697722.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFNKID"
     gene            complement(525..1268)
                     /locus_tag="KR440_000658"
     CDS             complement(525..1268)
                     /locus_tag="KR440_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBH0697723.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
     gene            1434..3999
                     /locus_tag="KR440_000659"
                     /pseudo
     CDS             1434..3999
                     /locus_tag="KR440_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            4229..4633
                     /locus_tag="KR440_000660"
     CDS             4229..4633
                     /locus_tag="KR440_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0697724.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            4657..5154
                     /locus_tag="KR440_000661"
     CDS             4657..5154
                     /locus_tag="KR440_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0697725.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            5157..5558
                     /locus_tag="KR440_000662"
     CDS             5157..5558
                     /locus_tag="KR440_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBH0697726.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            5576..6064
                     /locus_tag="KR440_000663"
     CDS             5576..6064
                     /locus_tag="KR440_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0697727.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            6091..6846
                     /locus_tag="KR440_000664"
     CDS             6091..6846
                     /locus_tag="KR440_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0697728.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            6846..7709
                     /locus_tag="KR440_000665"
     CDS             6846..7709
                     /locus_tag="KR440_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBH0697729.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKKKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            7756..8235
                     /locus_tag="KR440_000666"
     CDS             7756..8235
                     /locus_tag="KR440_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HBH0697730.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            8300..9607
                     /locus_tag="KR440_000667"
     CDS             8300..9607
                     /locus_tag="KR440_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH0697731.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETDMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVRGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            9918..10058
                     /locus_tag="KR440_000668"
     CDS             9918..10058
                     /locus_tag="KR440_000668"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HBH0697732.1"
                     /translation="MNGEGCILSNKITKECYKTGYMIREKSIDTYPDSGRGFMTWDEN
                     GE"
     gene            10570..11664
                     /locus_tag="KR440_000669"
     CDS             10570..11664
                     /locus_tag="KR440_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0697733.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQTQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSLLSENKEYKFLIEKLSLLEE"
     gene            11669..11869
                     /locus_tag="KR440_000670"
     CDS             11669..11869
                     /locus_tag="KR440_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HBH0697734.1"
                     /translation="MENLMEYLPLLIPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            11869..12786
                     /locus_tag="KR440_000671"
     CDS             11869..12786
                     /locus_tag="KR440_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0697735.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLEPDSGTIDVCGEKVTYGRTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIKIRSEELLSLVGLGNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNNEIQKFKSSGGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            12783..13541
                     /locus_tag="KR440_000672"
     CDS             12783..13541
                     /locus_tag="KR440_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0697736.1"
                     /translation="MREYIAFTKKEFKENIRNYKLLSLTILFLVFGIMSPLSAKFMPD
                     LIAHFAPTLKVTTAPTALDSWTQFSGNISGLGTSLTLIVFCNILSNEYSKGTLVIMLT
                     KGLSRSSVVLSKFSIAVIIMTIGFWLSFLCTYGYTMYFWPTANLNHIIFSAFNLWLIS
                     IMYISILILGCVLFRPAFASVLLVLAATAILSLISIPKQIAPYTPNFILSKNIDLVSG
                     KVTPPEFIIPIIVTIVISIVCLLLAVVLFNKKAV"
     gene            13924..14997
                     /locus_tag="KR440_000673"
     CDS             13924..14997
                     /locus_tag="KR440_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBH0697737.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            15067..15330
                     /locus_tag="KR440_000674"
     CDS             15067..15330
                     /locus_tag="KR440_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697738.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            15536..16513
                     /gene="bioB"
                     /locus_tag="KR440_000675"
     CDS             15536..16513
                     /gene="bioB"
                     /locus_tag="KR440_000675"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBH0697739.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSHYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMVFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEL"
     gene            16741..17754
                     /locus_tag="KR440_000676"
     CDS             16741..17754
                     /locus_tag="KR440_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBH0697740.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNSAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDVSVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            17796..18686
                     /gene="rbsK"
                     /locus_tag="KR440_000677"
     CDS             17796..18686
                     /gene="rbsK"
                     /locus_tag="KR440_000677"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBH0697741.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIKVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKKLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            18734..19696
                     /locus_tag="KR440_000678"
     CDS             18734..19696
                     /locus_tag="KR440_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBH0697742.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            19750..21261
                     /locus_tag="KR440_000679"
     CDS             19750..21261
                     /locus_tag="KR440_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBH0697743.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            21245..22243
                     /locus_tag="KR440_000680"
     CDS             21245..22243
                     /locus_tag="KR440_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBH0697744.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            22427..23689
                     /locus_tag="KR440_000681"
     CDS             22427..23689
                     /locus_tag="KR440_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH0697745.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIGGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFQMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARLMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            23709..24452
                     /locus_tag="KR440_000682"
     CDS             23709..24452
                     /locus_tag="KR440_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HBH0697746.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            24625..26010
                     /locus_tag="KR440_000683"
     CDS             24625..26010
                     /locus_tag="KR440_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0697747.1"
                     /translation="MDNLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KECLKKHGFSIEGLEGMDTAFVASYGEGRPVIAVLGEYDALDGLSQKVSTTKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFEYTPII"
     gene            complement(26347..26991)
                     /locus_tag="KR440_000684"
     CDS             complement(26347..26991)
                     /locus_tag="KR440_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH0697748.1"
                     /translation="MNNLDKNNVNEVLDMLLRTRRSIRSFTKEVPSKGLIEEIIETGR
                     LAPYAILSVAKQTDFRHFFVISNNSLQMNKIKTILVDVIKTQISVNEKKAKNNITLQS
                     FITSLKMQVEKGVLGIGTAPYLIIVAERRGIPPIEQKSLSHVMQNMWLKSTALGLGFE
                     LVSVIGRLSDNKDFCDILGVPTGEYGFDACAIGFPTTSVTREGRAIPHPSIKWI"
     gene            27255..27869
                     /locus_tag="KR440_000685"
     CDS             27255..27869
                     /locus_tag="KR440_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HBH0697749.1"
                     /translation="MKKVSIDRRTIRTKKMIRSALAELIDEKGFNNISVTDLTQRADI
                     NRGTFYLHYVDKYDLLERIENEIIQEIEEETKHLDSSNMMEIDTSSEPLPFMVKLFEY
                     FRKNSVIIKSILGPKGDPTFERKIKKFIETNLFEKQKLINFNIDEAIISEEYFIQYIL
                     SADLGVIQYWLEKNMKESPEEMALILARMSLLGPLRAVGIRKDS"
     gene            28090..28230
                     /locus_tag="KR440_000686"
     CDS             28090..28230
                     /locus_tag="KR440_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697750.1"
                     /translation="MKNENISYLALGLCFGVVLGSLFKNLAIGLSFGLVIGVVLDSKK
                     NK"
     gene            complement(28460..28907)
                     /locus_tag="KR440_000687"
                     /pseudo
     CDS             complement(28460..28907)
                     /locus_tag="KR440_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002599264.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            29677..30516
                     /locus_tag="KR440_000688"
     CDS             29677..30516
                     /locus_tag="KR440_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697751.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            30506..30949
                     /locus_tag="KR440_000689"
     CDS             30506..30949
                     /locus_tag="KR440_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697752.1"
                     /translation="MYSNTYNNENFMRARAFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            30960..31451
                     /locus_tag="KR440_000690"
     CDS             30960..31451
                     /locus_tag="KR440_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697753.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
BASE COUNT        12464 a         3263 c         5764 g        10234 t
ORIGIN      
        1 atataatatg agtaaatata gttctttatg ggaatatgtt aagaaaaata atagtcaatc
       61 gtttaaatta acttttgaag aaataaaaga tattgctgga atagagatag accattcgtt
      121 tttgaaatat aaaaaagaat taaatgaata tggatatcaa gtaggaaaaa tctctttaaa
      181 agagaagact gttattttca ataaaattga ttaagatata aataattaca tataataaaa
      241 taatttgtat tgacgattat tacaaataaa aataaagtaa atcttatata agatttactt
      301 tatttttaga tgatattttt ttagctttat actataaggt atataaaact aagcctatta
      361 aaaatgttat aatttctatt accatcatag accatatcaa tccatcaagt tcataaaagt
      421 aattaagctt tattaaatag cttctttatt ataatttctt tatttcatat aatataaata
      481 gtattaaaat ttttatggga gttaaagtaa gcatgtatta ttacttagat atttgtataa
      541 tcaacatcta gtaatttacc tttttggtga aaatgattaa taagtttttt aggataattc
      601 attattaatt catctggaaa attaatttcc tttaagatat tctcagaata ctcatagtta
      661 cctatgtcaa aacaaatgtg agcatctgaa gttagtatga ttttagcacc atattttttg
      721 caaagtgtag caatatactt acagttatcc gagctaccat ttcttgacac gcctttaata
      781 gaacaattat ttatttctat taagatattc ttttctatag ctaatttaat gattaattca
      841 tagtcgattg ggtagtttgg gtttccaagg tgtcctaata tttcaatatt atcatgctta
      901 tttattgcat ttattaaagc tttagtatta ttctctaatg tatttggaga aaaaacagct
      961 tcgtgaaatg acaagataag ataatctaac cgaggaatta caaaaggctc taggtctatg
     1021 tttcctttaa tatctagtat atttgcttca cagcctctta aaattataat gttatttatg
     1081 attcttggga tattttgaat gttgtgaaag taccatctgt ggggagaacc aggcatctta
     1141 ggaccatgtt cagatgtacc tagtatctta attccatttt ctttacaata attaatattt
     1201 tctagtaaag tgctatagcc atgaccactg actatagagt gggtgtgtaa atctgataaa
     1261 aaattcatat aaattcctcc tctataaaag atgagggtaa cgtaatttat attataaaac
     1321 gaataggaaa gaaaatcaat agttacataa ttagacaaaa atattaaaat attgtagaat
     1381 taagcttaat aataaatgtt taacagtatt actgcttata ggggaggtta actatgacac
     1441 aaaagaagct atcaaggaaa gaaaaagttt taatttgctt gaaaaggtta acagaagaac
     1501 acttgaactt taataatggc attagatttg gatttgatgc agaatatata gctactgaag
     1561 taggtataaa tagaagtaat gctagtaagg aattaaatca attaataaaa gaaagtatta
     1621 ttataaagat aaagggtaag cctgttcagt acttatataa agaaagtatt gagagtattt
     1681 tgggaaagaa actaaataaa gggatatttg ataatattaa ctctataaac gaatatagtg
     1741 aaaatgattc tatcaaagat gagtatattt tagagagtat aaaaaaagag aacaatattt
     1801 ttaatttagt aggatgtgat ggaagtttaa ggtcccaaat agaacaggct aaagcagcag
     1861 taatatatcc acctaagggg ttgagtacat taataatagg acctacagga gttggtaaat
     1921 ctatatttgc agaatatatg tataagtata gtttaactat aaaagaagat agtgataatg
     1981 caccatttat catatttaac tgtgctgatt attctgataa tcagcaatta ttattagcgc
     2041 agctttttgg atatgtaaaa ggagcattta ctggagctga taaagataag tatggtattg
     2101 tacatgaagc taataatgga gtattgtttt tagatgaagt tcatagatta tctgcagagg
     2161 gacaagaaat gctatttctg cttatggata aagggatata gaaggttagg agaggctaat
     2221 aaggtacacg agtgtagagt tatgataatt gcagccacta cagaagaccc agaaacagca
     2281 atgttggaaa cgtttttaag aagaatacct gtaactataa agattccgtc tttagaggaa
     2341 agaggctttc aagagagaat gaagcttata tgttattttt ttaaagaaga gagtgttagg
     2401 attggtgtta agttaaaggt ttcaaaagaa gttattaagg catttatttt atataagtgt
     2461 aagggaaaca taggccaatt aaaaagcgat atacaattaa tttgtgcaag agctttttta
     2521 gattatatga cctataaaag ggaatgtgta tatgtaaggc tctctcttct accaaataat
     2581 attagagaat cattatatgg aaataataga aaagaggagt ttgtacaaaa tttcagtttt
     2641 attggaaaag atgatattat ttttttacca gaagaaaagg attttgaaag tgaaaattta
     2701 ttaatagaaa ataaaaaata tgatttagat ttttatggaa tgataaaaga aacttggaat
     2761 aaacttcaac aagaaggtat taaagagtca caaattagaa gtgttataga tggagacata
     2821 cagaagtatt cctataattt aatgaatact tttatatata ataatagtaa tcaaacagct
     2881 tataacaaca ttgttaatga ttcaattgca actactgtaa aatatatttt acaaaagcat
     2941 gataaatgga gcaaaagaga aggattagat aaattaataa aagctatagc cttacatatt
     3001 cagaatttaa ttgaaagaat aaagattgga aatatagtaa agcatcctaa taaagatgaa
     3061 attatgaagg aaagagcata tgaatataac atagcaaaag aaatattatc taacatgtca
     3121 ttaatgtatg gagtagagtt tccagaagat gaagcaattt ttttagcaac atttctttat
     3181 ttatcgtatg tgggattaaa tgaggagagt atagcaatat tagtcataat gcatggtgaa
     3241 tctacagcat ctagcatggt gaatgtagcc aatactttac tggattgtaa ccatgcagtg
     3301 gcaattaata tgggattaga agatagagtt caggatattt tagtacaagc tgtagaaata
     3361 gccaataaaa ttgataaagg aaagggaatt ttaattttaa ctgatatggg gtctatttta
     3421 acatttgcta aagttattcg tgatgctaca ggaaaagaag taaaagcagt agatatggta
     3481 agtacaccta ttgtaataga agcaactaga aaggctctaa ctccagaaat gacattagag
     3541 aaattatatt ttaatattat agaatctata aagaaacatt atggagatat ggaagttaca
     3601 tatgctgaaa aagataatgg tggtagatat tttgatagac tactaattga taatattagt
     3661 aaaactttga cttttttaaa tggggaaaag gcttatttta tattaaaaga agtaattaat
     3721 agaattagtg aacactattc tttagtaatt caagatgaac ttttagtaaa gtttatattt
     3781 cattgttcat gtatgattga aagagtaata ataaaagagt ctttagttta taaatcatat
     3841 gaagaaagaa ttagtagaaa taaagaatta tatttagtta taaaagaaag ttttaagcta
     3901 gtagaagaga cattcgatat aattatttca gatatggaat atgcaaatat attagatata
     3961 tttgaatcac aatatgatac agataaattc aaaagttgat acaaaattta aaaatagcca
     4021 aaaggctatc ttttttttac tttttttgat acagatttga tacaggttga aaaaatcttt
     4081 tgatacatat cgaaataata agtttttaaa aaaaattgag taaaaagttt tgattaaatc
     4141 ctgtaaaata gccatttatt attgatacac ataagttggc acggcaattg cttatataaa
     4201 aagtgacaag aaaagtgagg tgaaattggt gataggaata atagttatga ctcatgggag
     4261 ttttagtgaa gaaattataa aaagctgtga acttattgca ggaccagcag aaagaacagc
     4321 agcaattaaa ctaaatagga atgataatat tgaagactta aataaaaatt ttgtagaaaa
     4381 gttaaatgaa ttagatgaag gtgacggagt tttagtatta gctgacctat taggaggttc
     4441 gccaagtaat gttgcaagtt taaaccttaa aaaaggtggc aagtttcatg cacttactgg
     4501 agtaaatctt ccaatgctac ttgaagcttt aattaataga gagggaaaaa gtttagaaga
     4561 gttagcagaa gcatgtattg aagcaggaca aactggtata aacaatatta ataaagttct
     4621 tagctcaatg taatagaaaa aggagtttga ttaaaaatgt ctttagtaaa atattgcaga
     4681 tgtgatgata gattaataca tggtcaagtg atatataaat gggttaagca tctaggtgta
     4741 aaaaagatag tagtagtaga tgatgaaaca accaatgatg ttattgctaa aggtttgata
     4801 aaaatggcag caccaaagaa tattgattta tcaatattaa ctgtaagtga atcaaggaga
     4861 tatttttata ataatcaagc tgatgataac gtttttgtat taattaaaaa tttagatact
     4921 gcaaatagaa tgatagaaga agggattaat atcaaaaaac ttattgttgg aagaatacct
     4981 acaggaatag gaaaaaagaa aatatctcaa aatgtataca ttaataaaaa agagtttctt
     5041 ttaatagatg aatttattaa aaagaatatt aatgtatcaa ttcaaatggt acctgatgaa
     5101 gaagaagtag aattaaacca aaatattaat cgctataaag gagagtttat ttagcaatga
     5161 ataaatataa ggtaattata ctgtgccatg gaacttgggg aaaagtctta gtagaagaaa
     5221 caaataaaaa ctttggattg tcaaatcaat atgaagtttt atcactaagt tctgaaaaag
     5281 atgtttctgt atttatgaaa gaggttgaag aagtagtaga taaagaagat gtgaaaatta
     5341 taatatcaga tttatatgga ggatctacat caagtgttgc aatagctgtt gctataagaa
     5401 gaggaataga tgcaataaat ggacttagct tacagacaat tttaatcgta gatgaagaat
     5461 taagcaaaga agataaatta catacattac cagaaagaat cgtcaaaaaa aataataacc
     5521 tttgtgtgga tttaattaaa gaatttaaaa aaatataaat tatgaggtga atataatggc
     5581 aaaaataaca ttagtaagag tagaccacag attaattcat gggcaagtta taacaaaatg
     5641 ggtaaaaatc gctcaagctc aaaaaataat aatagttgat gacttcttag gtcaagatga
     5701 atttatggct gacatatata aaatggcagc accatcagga gtagaagtag ttattttaac
     5761 agcagaagat gcgggacaag cattccaaaa caacacttta ggagataaaa atatatttat
     5821 tctatttaaa aatgttgata tggcaaataa ggcttataaa gctggactta aatatgaaaa
     5881 gattcaatta ggaggtattc caaatgaagc aggaaagaaa atggtgttta cagctgtttc
     5941 attaggaaat gaggatgtag aacaacttaa tgaacttaat gaaaatgggg ttgaaatagt
     6001 cctacaagtt ataccagagg aatcatctat gacatatgaa aatgcgttaa agaaatttaa
     6061 ataaaaaata taaattagga ggaattataa atggaatctg ttttgatgtt ggcaattgta
     6121 acaggattat ggtattggtt tgcagcaggt cttgcagggt ataccctttt ctcaacacta
     6181 aagtctcctt tatttatagg ttttagcctt ggattacttt ggggtgatgt aacaactggt
     6241 atgattgttg gtgcaagtat agaaatggtt tatctaggaa tggtcgcagc agggggaaat
     6301 attccatcag ataaatgtct agctgctctt attgcaatac cagtagcact tcaaactggt
     6361 gttaatgcag aagttgcagt atctattgcg gtacctttag gtgttatagg agtattagtt
     6421 aataacttaa gaagaactgg taatgctgta ttggttcata aggcagataa gtatgctgaa
     6481 gaaggtaata caaaaggcat ctggagatgt gcaacacttt attctttaat atttgggttt
     6541 gttttaagat ttccaatagt ttttgtatgt aacttttttg gagcagattt agttcaatca
     6601 ttattagatg ttataccaca atggttaatg aatggattaa cagtaatggg cggtatatta
     6661 cctgcattag gttttgcaac aacaatattt actataggta aaaataaatt cttaccaatg
     6721 tttattatag gattttttat ggttcaatat tttgaaatat caattactgc agctgcaata
     6781 tttggtgttt gtatagctct tttaattacc tttatgaaag aagacaaaag ggtaggtgaa
     6841 gtataatgca agaaataaaa aataaagaag ttaaaaatgg acaaggtcca actgaagtta
     6901 ataagacaag agtactcaca aagaaagatg taacaaaaac atacctaaga tggtggtgga
     6961 cagcagagtt atctaattct tttgaaagaa tgcaagcatt agcagtttgt gcaagtttca
     7021 ctccggcttt agaaaagtta tataagaaaa aagaagattt agtagatgca ttaaagagac
     7081 acttacaatt ttttaatact caagctatat ggggaggatt aattcatggt acagttttag
     7141 ctatggaaga agagaaagct actgaaggaa aaatcccagg agaagttatt tcaggtgtta
     7201 agaatggtct tatgggacct ttggctggaa ttggagatac tttagatttt ggtacatttc
     7261 aaacaatatt cttagcttta ggtgcttcat ttggagcaga aggaagtgtt ataggtgcat
     7321 ttttcccaat aatgtttagt atattactat tttgcgaagg ttattactta tttcatttag
     7381 gatattcttt aggtagagat tcaattaaga aaattctatc aggtggtatt gtcaataaga
     7441 taattgatgg tgcttcaatt ctaggaatgt ttatgatggg agcattatct gcaactacag
     7501 ttaagctttc aactccgcta tcatttgata ttggtggtaa agcaattgtt gttcaagata
     7561 cattaaatat gattgctcca ggtttattac cacttggtgt tgtattcttc gtatattggg
     7621 gaatgaaata caagaagtgg acaataacta agttattagt aattttagtt gtattagcat
     7681 tggtaggatc attcattgga atattctaat tcacagtgat taaaatatag aatttaattt
     7741 taggaggaga aagaaatgtc agaaaaaatg tatacagaaa aggaatattt agaaggaatt
     7801 agaggagcaa caggagaaag agcagtatgg ttctatcttc ttatgaaaga agctgaaaaa
     7861 ttaggagcta atccagatga tatatgtaaa gaagcaatat atggatttgg taaaatgaga
     7921 ggtcaaaaat ataacgtagc agatactcca ggaaaaatgg cagaaatgtt atataacagt
     7981 aaaggacaaa aagtgtttga aatggaatta gtagaaaata cagatgagaa tggcgtttta
     8041 aaattccatc actgtccatt agatgctgct tggaaagaat atggattaac taaagaagaa
     8101 agaaaagaaa tttgtagatt agcttgttat ggggattatg gaagagttga ctgtgcacaa
     8161 ggtgttaaat tagattttgc tcaaaaatgt gctcatgatg atgaagtttg tgaattagta
     8221 tttacaaaaa aataattttt aatgctaaaa gcctgttctt taaggacagg ctttaaaaag
     8281 atagaaagta ggttgattta tggatataag cagaagtgta gataatagtt taaatagaac
     8341 catagaattt ctaaaagaat tgataaaaat tgattcacaa caaggtgaac ctatttcaca
     8401 atgtcctttt ggtataggac ctaaaaaatc tttagataaa actttagatt actgtgctag
     8461 tttaggtttt agtgttaaaa atattgataa ttatattgga tatgcagaaa ttggagaggg
     8521 agaggaatta atagggattc caatgcattt agatatagtt ccaccaggag aggggtggag
     8581 tgtagaccca ttttcgggag cagtaattga caatattatt tatggaagag gtgtcataga
     8641 taataagggt gctgtatcta tgcttataca tgtattgaaa aacatagagg atatgtatcc
     8701 tacaattaat aaaagaataa gattaatttt tggtacaaat gaggagacag atatgaaatg
     8761 tatcaaatat tacttagata aaggtgaaga aattccaagt atgggattta ctccagatgc
     8821 tatgtatcca gtggttaatg gagaaaaagg aagggttcat ataaggatag aaaaagaaat
     8881 taaaattgac aaaagtaaac cttacataat agtaagggga ggaactaaag aaaatgttgt
     8941 tccatctcat tgtactgcaa aaattattaa tggaattatt tcagagttaa ctactaaagg
     9001 agtagctgta catgcaagta atcctgaaaa gggagaaaat gctatatcta aaatggtaat
     9061 aaagatagtt gaggataata tggattttca gcatagagaa gatatagaat tagtttcaaa
     9121 atacctttgt agtgattatt atggagatgc attggggatt aatcaatatg atgaggtatt
     9181 caaaaacact accttaaatt taggaatatt aaaagtaaat gaggaaaaaa tcgtatgtga
     9241 gttagatata aggtatggta aaaatatagt tttaaataac ataatagata gattcaaaaa
     9301 ggttttttgt aatggttgga aaatagaggt tattgctcat aaagacttac attatgtgga
     9361 tgagagtaat ttagtactta aaaaattatt agaagcctat gaagaagtaa cagatgaaaa
     9421 tggatataca attgctatgg gtggtggcac atatgcaagt tggtttaaag atatggtggc
     9481 gtttggacct aagttcttag cgtataaaac tggtggtcat ggagtagatg aaagagtacc
     9541 aataaatcat atacgaaaga atatggagat atatactttg gctttaataa aactattaga
     9601 attgtaaaag aagaagagac tttatgtgca aatttacaga tatcttaaaa agagagataa
     9661 aacgagttca actttattta aatgttcaac accatcaaat ggggctgtct caaaatagag
     9721 ataagcctct tgcttattat tgaaatgatg aaaatatatt ttacttcata caaaaaagag
     9781 tgccttatga agtaaaaagt taattttgag acaccctcat ttgatgatgt tgaagagaaa
     9841 ataagtatga aattattaaa aaaagatgat tttataaaag ttaaggttga aaataaaagt
     9901 taattgaatg gaattagata aatggagaag gctgtatact atctaataaa ataacaaaag
     9961 agtgttataa aactggttat atgattagag aaaagtctat tgatacttat ccagatagtg
    10021 gacggggatt tatgacttgg gatgaaaatg gagaataata aaaattatag tttatacaat
    10081 ttaataagat atatgtacaa gcagttaatc taaatgattg actgcttttc ttttatagat
    10141 ttttatataa tagacaatta ctaatacatg atagttgcct gttataatta gaggaataga
    10201 tacgaataat aactttataa aagaattaaa tggaaaattg aagagttata ttcaggattt
    10261 taaaaagaat atgaaatgag aaatgaaaat aaggtgttta tattaaaaga ggttttacac
    10321 aaaatactta aaaacgtgat aaataaaaac tgtatttaga taaattaact actagtttta
    10381 tatataatat ggattttgtt tacaaagttt acatatttca caaaatattt ttaaaatata
    10441 tttaaaaata tattttatat acttgttcgt ttgtttctct gtggttgatt ttttcgtcaa
    10501 aagtcaactt caaatcgctt gttagcttta catgtttaag gatataatta tacataagga
    10561 gggattgaca tgaatgaatt aaacatcgca aaaacgctga ttttaaagcg taaagaaaaa
    10621 ggaattacac aagatgaact tgcaaattat ataggcgttt ctaaagcttc tgtatctaaa
    10681 tgggaaactg gacaaagcta tcctgacata acctttttac cgcaactcgc gacatatttc
    10741 aacattaccg ttgatgagtt aatatgttat gaacctcaaa tgatgaagga agatattaat
    10801 aaactgtata ataaactgtg caaagacttt acggttaaac cgtttgatga agtcatggat
    10861 gaaataagag aaatcataaa aaaatattat tcatgttttc ctttgatttt tcggatgggt
    10921 cttttaatag ttaatcacta tgatattgta gatgaaaaaa agcgtgaact attaattagt
    10981 gaagcattag aaatatttat tcgtatacag gaaacttgta atgacataga tatttgccgt
    11041 caagcaaaaa gtatggaggc aacttgttat atattgttaa atcaacccat acaagtgatt
    11101 gatttattgc aaaacagcaa ttttcccatg ataaatgaat ctatacttct tgcacagggg
    11161 caaatgatga atggtcaaat ggatgaagca agagaaacat ttcaacttgg tgcatatcag
    11221 aaccttatat cattagtcca aaatctagtg ggtattttgc aaaatgcaga taaattacaa
    11281 atgaaggaaa tagaaagacg tattctagca atttcagata tatttgagct tgatacatta
    11341 tctcctgcta ttatgctttc ttcatattta acacagacac aaattaattt aatacatggg
    11401 gataatgaag gagctattaa atcattacga aaatatgtag atttagcaac acgtgatata
    11461 tacccaatta taatacatgg ggatgacttt tttaataaac ttgacagatg gatttctgaa
    11521 attggaacag gtattacacg tgatgataca atcgtaaaag caggtattgt tgctgctatt
    11581 aaaaataatc caatgttttc tttattaagt gaaaacaaag aatacaaatt tcttatagaa
    11641 aaattatctt tattggagga ataagattat ggaaaatttg atggaatatt taccactttt
    11701 gattcctgtt attattttag atttaatact tataattact gcacttgttc atgtattgag
    11761 acatccaaat tataaaattg gaaataaggc aatatggatt attgtagtac tgtttattag
    11821 tctaatagga ccaatcttgt attttacaat aggaagaggt gaagaatgat gaatattgtc
    11881 tcaataaatg gattatcaaa aggttttggt aatcgaaaga taatagataa tctaaatttt
    11941 actgtaccag aagggtcagt ctttgggttt gtaggtaaga atggtgcagg taagactaca
    12001 acaatgaaaa tggtacttgg gttacttgaa ccagatagtg gaactataga tgtttgtggt
    12061 gagaaagtga catatggaag aacaagttct aatcgtcatg taggttatct gcctgacgtt
    12121 ccagagtttt acaactatat gagaccactt gaatatcttt ctctttgtgg tgaaattacg
    12181 gggctttcaa aaaaagaaat aaagataagg agtgaagagt tgttatcact tgtggggctt
    12241 ggcaatgaaa agagacgaat aggtgggttt tctcgtggaa tgaagcaacg acttggtata
    12301 gctcaagctt tgctttcacg tcccaaatta cttatttgtg atgaaccaac aagtgcactt
    12361 gaccctgttg gtcgaaaaga aatattagat attatgctta aaatcaaaga ctcaacaact
    12421 gtaatatttt caacacatat actatcagat gttgaaagaa tctgtgacca tgttgctata
    12481 ctaaacaaag gaagcattgc tcttagcggt acactttcag aaataaaaag tatgcacgga
    12541 aaggatagac ttttactgga gtttgcaagt aataatgaaa ttcaaaagtt taagtctagt
    12601 ggtggcatta aatccttact aaaagattca gaagaaacta atatggaaat tgttttgcat
    12661 ggtaaagata taaaagcaat acaaaaaaca gttatatcta cactcgcaga aatgaatctt
    12721 tgtccagtaa agatggaact aatagaattg tcacttgaaa atttgttttt ggaggtagta
    12781 aagtgagaga atacatagca tttacaaaaa aagagtttaa ggagaacata agaaattata
    12841 aattacttag tttgactatt ttatttttag tatttggaat aatgagtcct ctttctgcaa
    12901 aatttatgcc agacttgatt gctcattttg ctccaacttt aaaagttact actgcaccaa
    12961 ctgctttaga ttcttggaca cagttttctg gaaatatttc tggtcttgga acgagtttaa
    13021 ctctaattgt tttttgcaat attttatcaa acgaatattc taaaggaaca ctcgttatta
    13081 tgttgactaa agggttatca cgttcatctg tagtattgtc taaattttct attgcagtaa
    13141 ttattatgac aattggtttt tggttgagct ttttatgtac ttatggatat acaatgtatt
    13201 tttggccaac tgcaaatctt aatcatatta tattttcagc atttaactta tggttaatta
    13261 gtattatgta tatttccata ttaattcttg ggtgtgtact atttagacca gcatttgcaa
    13321 gtgtgttact tgtattagca gcaactgcta ttttgagttt gatttctatt cctaaacaga
    13381 ttgcacctta tacgcctaat ttcatattat caaaaaatat agatttagtt tcaggtaaag
    13441 tgacacctcc agaatttatt attccaatca ttgtaacaat tgttatttcg atagtatgtc
    13501 tgttattagc tgttgttttg tttaataaaa aagcagttta aggatatttt tattataaat
    13561 acaaatagtg ctgtattgtg ggaaaatgtg tttagtattg tagaagaata tatttaagta
    13621 aaaaagaggc tgtctcaaga tagagaaata agcctctttt ttattttagt cctcctcact
    13681 atactttata ataattaagt tattacctta aggaactatc taaaaaaaga gatatagatt
    13741 ataatctaat gcttatcagt tatttgaggt gtttagtata tagagtctaa agtagtgaag
    13801 gactaaaata atattgtttt cattaaataa agataagaat ctttaattgt ggtaaaatat
    13861 actaaaaaat cattttaaaa gtgataaaat tattttaagt aaatatttta aaggggtgta
    13921 gatatggaaa agtcaaaagt atatttttgt gatttatact caaattcgca aaataaaaac
    13981 gtaccaaaca atgtaagaag attatttgat gaagcaggat tcaaagattt aatagaaaag
    14041 aatgaccaag tcgcaattaa acttcacttt ggagaaaagg gaaatactac atatatgagt
    14101 cctgtggcag ttagacaagt ggttgataag gttaaagact gcgaaggaaa gccattttta
    14161 acagatacaa atacactgta tacagggagt agaacaaatt ctgtagacca tcttacaact
    14221 gctatagaaa atggatttgc ttatgcagtt gtaaatgcac cagtaataat tgcagatgga
    14281 ttatatagta gaaattatga gaatgtaaaa atagacaaaa aacattttga aagtgtaaaa
    14341 ataggtggag aaatttataa ttcttctgca atgatagtaa tgagtcattt taaaggtcat
    14401 gaagcagcag gttttggagg agcattaaaa aacttagcta tgggatgtgc aagtgcagca
    14461 ggaaaacaaa tgcaacattc tgatgttact ccagtagtaa aggaaaagaa gtgtgtaggt
    14521 tgtggaaagt gtgttaattc ttgtcctaca aaagctataa gtatagtgga taaaaaagca
    14581 gttatagatt ctgatgtatg ttatggatgt ggagaatgtc caactgtctg tccaactaga
    14641 gcagtaacta tacaatggga aagtgactca gatgtgtttg tcgaaaagat ggcagaatac
    14701 gcttatggag cagtatcaaa taaaaaagac aaggtagggt atataacttt tgttatgaat
    14761 gtaactccac tttgtgattg tgtaccttgg tcaggtagac caattgccca tgatatagga
    14821 atattagcat caacagaccc tgtagctatt gaacaagcat gctatgattt gatttgtaag
    14881 gaaatgggtc atgatgtttt caaacatgaa catcctcatg taaatggaac tagaataata
    14941 gactatgcat gtgaaatggg tatgggaagt aaagaatatg aattaataaa attataagaa
    15001 tagtagcact taaatgcata taattattgt aagagaaatc taaaagtatc aaaggagtat
    15061 gcatttatga aaatttacta tgagaaggaa aacagcaaga aggctaatgt aaacaatatt
    15121 ccaagtgtat ttgcgtttaa ttttctaaga aactttttga ctagcaaaga gtatgaggaa
    15181 actagaaaag aatattttat aaataatcta acaaaaagtc aggttaatca agccatgaag
    15241 gatttaaaat ggctatttag agaatatgaa ggcttagagg ttattacaat ggaaagcttg
    15301 gatggagtta agacaaggat agttttataa accagtaaga attatattct aataaaaaat
    15361 actgctttaa taataatatg tataatctat atactaatat tattttgaag cagtattttt
    15421 ttgtgtatag taaacttgtg tctaaaataa agttgaccaa taaatgttat atatgcaata
    15481 ctatatataa taagtaaact acataaaaat attaagttga caaataggag gatatatgga
    15541 aaaatacata ataaaattaa aaaataaagt attgagagaa aaagaaataa gttatgaaga
    15601 agctcttaat ttaataagcc ttgatacaaa taataaaaac gattttgata ttctattaaa
    15661 atctgcaaat gaaataagag aatattttat gggaagaaaa gcagatttat gtacgataat
    15721 gaatgctaaa tctggcaaat gtagtgaaga ctgtaaattt tgtgctcaat catctcatta
    15781 taaaactggt gtagaagaat attcattatt agattacaat gaaatcttaa atagagctaa
    15841 agagatggag agtaaaggag tgcatagatt ctcattggtt acaagtggta aaggtatgtc
    15901 aggaaaggag tttaataata ttttaaatat ctatgaagga ttgagggaaa atactaattt
    15961 aaaattatgt gcatctttag gtattataga ttatgagcaa gctaaaatgt tgaagtcagc
    16021 aggtgtgact acatatcacc acaatgttga gacgtgtaga gataattttc ataatatatg
    16081 tacaacacac acatataaag ataggataaa aactataaaa gatgctaaaa aagctgggct
    16141 agatgtatgt gtaggcggta taattggaat gaatgagagt gaagaacaga gactaaaaat
    16201 ggtttttgaa atacgagaat taaatgtaaa atcatttcca ataaacattt taaatcctat
    16261 taagaatacg ccaatggaaa attatgatgt attagaacca atggaaatac taaaaacaac
    16321 agcagtattt aggtttataa ttcctaatgt gtacataagg tatgctggtg gaaggctatc
    16381 attaaaggga tatgacaaag ttggatttaa tgggggagta aattctgcaa ttgttggtga
    16441 ttatttgaca actgttggaa gtgggattga aaatgataaa aaaatgatta tagagcaagg
    16501 atttgaactt taataaatac tacttaaatt aattaatata cttttagata attataaaag
    16561 tatattaatt ttttttgcag attaatttaa atattaaaat tgaattataa gttgcaatat
    16621 tttgaaaaaa gggaaaaata tcaaaattta tataaaatgg ttgcaaatgg gaaaaggatg
    16681 tgatagtatc taggtaaccg gttaagtaaa acggtttcct aaattttggg ggtgaacttc
    16741 ttgagtataa ctataaaaga aattggcgaa ttggctggtg tttctaaaac aactgtgtca
    16801 aaagtcatta ataataaaga tgaaaatata agtcaggcaa caagagaaaa aattttgaaa
    16861 ataatgaagg aaaaaaatta tgtaccaaat aaattagcac aaagtttagt aactaaaaaa
    16921 actaatacta tagggcttct tataccagat atacgaaatc cattctttac agatgtatca
    16981 agaggtgtag aagacaaagc taatgaagaa ggctacaata taattttatg taatacagat
    17041 gaagatgcta aaaaagaata tgaaggaata agaacactat cagagaggat gatagatgga
    17101 attatatttg cagcatcttc aaatacaaac tggaaagaag caaattataa agatataaaa
    17161 ataccaactg tcttgattga taagaagata agtatgaaca aagaaacctt aaaaggaatt
    17221 gttaaaataa ataactttga aggggcctat atagctacaa aacatttatt agatattgga
    17281 aataagaaaa taatatacct aagtggtcca cttcaaaatg aaatagcagt agatagatta
    17341 gaaggatata aaaaggctct aatcgagtgt aatttaagct acaattctgc ttatgtattt
    17401 gaaggaaaat ataagattga atggggacaa gaatttataa aaaatttaga aaagatagat
    17461 tttgatgcaa tattttgtgc aaatgatttg attgcaatag gtgtcataag aggtcttaaa
    17521 gaaagagggt taagtatacc aaatgatgta agtgtcgtgg gatttgatga tattcaaaca
    17581 tcaagtctaa taagcccttc tcttaccaca gtaaaacagc cgtcttatga tataggatat
    17641 aaagccagtg aaatattgat aaattgttta agaggagata aaaaagaatc atttgatgaa
    17701 ttgattttca aaccagaatt agtcataaga gattcaacaa aagaaagtga ataacagcac
    17761 gagagacata aatagatatg taaaggagta atgtaatggg aaacatagtt gtcataggaa
    17821 gtgtaaacat ggacatggta tgttctgtag ataaaagacc agaaaaagga gaaacagtat
    17881 taggtaatag tttttttaca tcacctggag ggaaaggggc taatcaagct atctcagctt
    17941 ctaaactagg agcaaatgta aaaatgatat catgcatagg ggaagatggc ttaggagagg
    18001 agttaataag aaattttaga acgaataaag ttgattatag tttagtatcc agaaataatc
    18061 acaaaagttc tggtgttgct gttataacat tatgtgaaaa tgataatagt attgttgttg
    18121 taccagggac gaatgagcta gtagatatag aattaattaa aaagaatgaa gaagagataa
    18181 agaatgcaga tatagtattg ctacaattag agattccatt aaaaacaata aattatgtag
    18241 tgaatttctg ttttgaaaat aggattaagg ttttattaaa tccagcacca gcagtaaaac
    18301 taaatgaaga tataatagaa aaagtaactt acttaacacc aaatgaacat gaatataaga
    18361 tagtttttga cacaaatgaa gggatagaag aagtattaaa aaaatatcca aataaacttg
    18421 taataacaga aggaaaaaat ggagctagat tttatgatgg tgaggaaatc aagcatgtat
    18481 cttgtataag tgttgatgtt caagatacca caggagcagg agatacattt aatggagcat
    18541 tggcagtggc tataacagaa gggaaaaaat tatatacagc agtagaatat gcagtagtag
    18601 tatcaggtct ttctgtaact aaactaggtg cacaatctgg tatgccttat aaagaagatg
    18661 ttgaaaaata tttaaataat aaataaaaat ctaatttgtt caaatttaaa actattaaaa
    18721 atggaggtta attatgagag tgagaaaaat tttatcttta ggaattgctg gaatactagc
    18781 aataggaatg ttaacagggt gttcaatgga aggaccaagt aaatcagata ataaaggtgg
    18841 ttcagacaag aaagatttaa ctattggagt atctacaata actcttcaac atcaattttt
    18901 tatagatatt gatgagggaa taaaagaaaa agctaaggaa ttaggtgtga aagttatagt
    18961 aaatgaccca gaccaagatg tagctaagca aacatcagca atagaagatt ttatacaaca
    19021 aaatgtagat ggaatgatag tacttggaac tgataattcg gctatagtac cagcagtaga
    19081 aggagcattt gaaaaaatgc cagtagttac agtagatgct gtattaaata ctgaaaatat
    19141 tacaagttat gtaggaacag ttagttatga tgctggtaaa aaattagggg aatacactaa
    19201 aaaatacata gatgaaaagt taggtggaaa gtcagaaata gcaatagtga cagatttaaa
    19261 atctcaaata caaatgcaga gaatagatgg atttaaagat gcgttaaaag gttcagcaaa
    19321 tgttaagata ttaaattcac aaccaggata tgacagagaa gaatctctta atacagtaga
    19381 aaacttaatt caatctaatc cagatgtaga tataatatat gcaacagctg aaaatagtgt
    19441 actaggagct aaagctgcac ttgaatctgc aaaaaataag gatgttaaga ttgtaggatt
    19501 tgacttaact gaagaagcat caagtggaat aacagatgga actattcttg ctatgattca
    19561 acaacaacct aaagagatgg gacgtttagc tgtagaagca gtagtaaaag ctataaaagg
    19621 ggaaaaggta gaaaagaata taccagtacc agctttactt tatgataagg aaaatataaa
    19681 agactttaag aattagtaaa ataaaatgag attatttgaa agaaatccta tttaaaaagg
    19741 agtgtcatta tgagtaatat aattctgaag ttaagtaata ttgctaaaga atttcctggt
    19801 gtcagagcat tagataatgt taattttgag ttatttcatg gcgaagttca tgcactactg
    19861 ggtgaaaatg gtgctggtaa atcaacaatg ataaagattt taactggtgc acattcgaaa
    19921 acttctggaa aatttatttt tgaaggcaaa gaaatagaga atatatcccc agatatctct
    19981 aaaaagatag gtataaatgc catatatcaa gaactaacag tttttgacga attaacagta
    20041 gcacaaaata tttttatggg aaaagaaata aatggcaaag tcttaaccaa tgacaaaaaa
    20101 atgaatgaag aggctaaaaa gattttcgat aatatgggaa tagatataaa tccaaatagt
    20161 ttagtcaaag agcttagtat agctcaaaaa caaatggtag aaatagcaag agttttatca
    20221 tcagaaacta aagtattaat aatggatgaa cctacttctt caataagtaa aaaagagact
    20281 gaaatacttt ttagacttat aaatgattta aaagagagtg gagttagtat aatatatata
    20341 tcccatagga tggaagaact ctttgagatt tgtgacagaa taactataat gcgagatggg
    20401 aaaacgattt cgactttgaa taccaaagat gtatcgtctg aggaagaatt agttaattta
    20461 atgatagata gaaagctaga ccaatttttc ccaaaaagaa aagtagaaat aaaagaagaa
    20521 attatgcgag tggaaaatct aactaagaac aatgttttta atgatattag ctttaatata
    20581 agaaaaggtg aaatattggg aataggtgga ctagttggaa gtaagagaag tgaaatagta
    20641 gaagctatat ttggacttag aacatatgat tctgggaaaa tatatttaaa caatgaagaa
    20701 gtaaagttta aaactccatc agatgcaata gaaaatggac tgggattaat aacagaagat
    20761 agaaaaggta ctggattgtt tttacaaatg agtgtcaaag aaaacacaac tatggctggg
    20821 ttaaaaaaga tatctaagtt taaatcaatt atagatagaa aaaaggaaaa agagatttta
    20881 gaaaaataca tagaagcttt aaagataaaa acaccacata tgaatcaagt tatacaaagt
    20941 ttaagtggtg gaaaccaaca aaaagctata atagcaagat ggcttttatt acaaccagat
    21001 atattgatta tggatgaacc aaccagaggt atagatgtaa atgctaaggc agagatatac
    21061 aatctaatgg gagatttagt ggagagtggt gtaagtataa taatgatatc ttctgaaata
    21121 ccagaattaa tatcaatgag tgacagaata atggttatga gagaaggtca tataagtgga
    21181 tttttagaag gtgaagaaat ggttgaaaat aatgtattaa aattggcttt cggaggtaaa
    21241 attaatgagt tcaataacta agcataaaga agaaaaatct tctccattat tgacaaaatt
    21301 aacaagtgta tttagagacc aagggagtgc agctgtagga cttgtaataa tttttataat
    21361 aatgagtgta gcttcatcta actttttaac tttagataat ttaattaatg ttggaagaca
    21421 aatatcaata aatgcaatac ttgcagtagg tatgacattt gtaataatta caggaggaat
    21481 agatttaagt gtaggagcag taattgcttt agtaggtaca ttttgggcta caacagtagt
    21541 gaattataat gcacctatat gggtaggtat gatacttgca ttagcaatag ggacaatatt
    21601 aggagtcata aaaggtgcaa ttatttcaac acaaaaacta cctccattca ttgtaacttt
    21661 agccatgtta acgataataa gtggggcaag ctttgtattt acaggaggaa gacctatatc
    21721 agtaaataca gatgcattta agatgctcgg tagaggatat attggaccaa taccaatacc
    21781 agttataatt atgattatag ttgtaatagc aggacatttt ctattaaaaa gaactaattt
    21841 tggacgtcat gtacatgcag taggtggaaa tgaagaggcc gctagattat gtggagtaaa
    21901 agtaaataaa gttatagtaa aagtatacgc actagctgga ctactaacag cattagctgg
    21961 gataatacta tcatcacgtt tggcatcagg ttcaccaact gttggagatg gagcagaatt
    22021 ggatgctata gcagccgttg tgcttggtgg tactaatatg atgggtggtt ctggttctat
    22081 agtaggtaca tgtataggtg tcggaataat aggaatatta ggaaatggat taaacttatt
    22141 aagtgtatct tcatataatc aaatgattat aaaaggtttg gtaatgttat ttgctatatg
    22201 gataaataat ataaaattaa aaaaatcagc taaatctaaa tagtaattaa attttagctt
    22261 ataaactata aaaattatta agtttttgta tatattttta tactttaatt tgcatacatc
    22321 tttatacatt gaatgggaat atgctaagtg aaaaagttta ctatggctta aatagatgag
    22381 attagacttg aaattttaaa tgtaaaaata aatcttagga ggaaatatga aaaaaattct
    22441 cttagataca ttaaatagta aaaaacaaga atacatagat tatttaaaag aacttgtaag
    22501 tataaagact gaagatgttg gtcatggaat actaggtgga tttgaaaaag aaggacagga
    22561 atatattgaa aaacttgcaa attacatagg ttttagtgta gatagacaag aaatgagcga
    22621 agaattaatt aaaaaagcaa aaaatatata taaagaagga aatctaggtc acaattatca
    22681 agatagatat aatttaattt gtaagtatag tgatgattta cctggtaaga caattgtatt
    22741 taatggtcat gtagacacta tgccaccagg tgatataagt aagtggaaat ataatccata
    22801 tcgtgctaca gaagataatg gaaaacttta tggtcttgga actgctgata tgaaatctgg
    22861 acttatagca tcaatattag ctgtaaagct tataaaagat agtggtctaa atgtacctgg
    22921 taatgtaaaa ataatgtcag tagtagatga agaaggtggc ggaaatggta ccataaatgc
    22981 agttatgaat ggtattggtg gagattgttg cataatttgt gaaccatcag aacaaaactt
    23041 aatagttgca catatgggat ttgtattctt tgaagttgag gttaaaggtg tgtctcttca
    23101 ctgtggtagt aagtgggagg gtgtaaatgc aattgagaag gctatgttgt tgcttcaaga
    23161 tataaaagag ttggaacata actggcttat gatatataag catccactat taccttcacc
    23221 tacaataaat ttaggtgtaa taaatggtgg aacagcaggt tctactgtgc cagataagtg
    23281 tgtatttaat ttatgtgtac acttcttgcc aaatataatg agttatgaac aagttgtaaa
    23341 tgatgtaaca aatgtcataa tgacaagggc taatggagat ttatggctta aagataataa
    23401 accaaatata aacatatatc aatcagggtt aggttttcag atggacaagg attctgattt
    23461 tgtagtgaat gcacataaaa tactagaaga aactttagga aaaaaattag agataaaagg
    23521 ttcaacagct ggcaatgatg cgagattaat gaaaaattta gctgaaatac caacacttat
    23581 attagggcca ggttcaatag aacaatgtca ttcaattgat gaatatgttg aaattaagga
    23641 gtatttagat agtatactta tgtatgcaag tctaatatta aacttataaa agtaggagga
    23701 atatataaat gaaaaaagta ttaatagtag gggaatcttg ggtaaaaaat ataactcata
    23761 taaaaggatt tgatacgttt gttacaactc attatgaaga agctgtaaag tggttgaaag
    23821 aagcaataga atctggagga tatgagacag ttcatatgcc tgcacatgta gcagcagata
    23881 gttttccgta taaattagaa gagttaaatg agtatgactg tataatatta tctgatatag
    23941 gttctaatac gttcttatta tctaatagta cttttataga ttgcaatagt aatccagata
    24001 gacttgaact aatcaaagag tatgtaaata atggtggggc gttaatcatg gtaggtggat
    24061 atatgtcatt tacaggaata gatgcaaaag ctagatttgg agaaactgct atcaaagatg
    24121 tattaccaat aacaatgata gataaagatg atagagttga aaaaccagca ggaataattc
    24181 cagaagttat tgactctgaa catccagtat taaaaggtat accaacagaa tggccaaagt
    24241 ttttaggata taacaaaaca gtagctagag ataattgtcc tgttttagca actattggag
    24301 gagacccatt tgtagctgtt ggcgagtttg gaaaaggaaa atctgctatc tttagttctg
    24361 actgtgcacc tcactggggt ccaaaagaat ttactgattg gaaatactat aataaattat
    24421 gggttaatat gcttgattgg ttaacatgct aaaaatttta taaaacttga aaacctctta
    24481 cttgataaaa tgaaacttac aagtcaagag gttttattgt ataaaatggt tgtatggtag
    24541 aaaatatttt taaatcagtt aaaattagtc attttggctg ataatcgata agttattaga
    24601 aacagggagg tttatataat taatatggat aatttaaata aaaagttttt gatggaatat
    24661 atagatgaga tacagggaga attaagagat atatcagata agatatggaa aaatccagaa
    24721 cttcagtata aagaatacta tgcttcaaat ttacaaaaag aatgtttaaa aaaacatggt
    24781 tttagtatag agggattaga aggtatggat acagcatttg tggctagtta tggagaaggt
    24841 agaccagtta tagcagtttt gggagaatat gatgctttag atggtttgtc tcaaaaagta
    24901 tccactacta aagaacctat tgtagatggt ggtgcaggtc atggatgtgg acataactta
    24961 cttggtacag gttcaatagg agctgtaatt gcagtaaagg aattaatcaa actaaaaaag
    25021 ctgaaaggga ctataaaata ctatggatgt ccagcagaag aagatttatc tggaaaagta
    25081 ttgatgataa aaaaaggttt ttttgatgga attgattgtg cttttagttg gcatccattt
    25141 gatataaata ctccgataag aatcccaaca cttgctaatt actctgtgaa atttagatat
    25201 aatgggatta gtgctcatgc agcacaagct ccttataacg gaagaagtgc acttgatgca
    25261 gtagaactta tgaatatagg ttgtaattat ttgagagaac atatatttga taattgtaga
    25321 gtacactatg taactactaa tggtggaaaa atgccaaata tagttcctga ttttgcagaa
    25381 gtgtggtatt acataagagg agttaaaatg gagcatgtaa gagatgtatt tggtaggatt
    25441 gttgatatag caaaaggagc agcattaatg actggtacta ttatggaata tgacattata
    25501 agtggagtat atgactatat tccaaataca atattaacag atattctatc acaaaatatg
    25561 aaattagtag gtgtgcaaaa ctataatgaa gatgactact attttgcaga taaattagct
    25621 gagacagtgt ctatagataa aagaagtagt gtttctagtg tattaagtgg aaataaggat
    25681 attactaaga tgaatttaca tgatgaggtt acagatgata catttactca taataactgt
    25741 atatcattat cattggatat tggagatgtt agctacataa ttcctacagc acaatgttct
    25801 tgttcagttt ggcctattgg tatttctgcc catacatggc aatcttgtgc cagtgcaggt
    25861 tctgatatgg gatttaaggc aatgttattg gcatctaaat ctatatcatg ttcaataaat
    25921 gatgtaatgc ttgatgaatt ggttataaat aaagcaaaaa aagaattgaa ggatactgta
    25981 ggtagttttg aatatactcc aattatttga agagtaattt aatacaagta agattgaatt
    26041 gtatgaatta ggatgaatat ataaattaat attattttta acttaacttt ctaattattt
    26101 tcaatctaag atacaataat tagaatatat ttttaaagtt tcctatttaa ggaaaataat
    26161 ttgattttat gtttgataaa aggacttata atttgtaagc ccttttatta tatttatttt
    26221 ttgtttataa ttaattttat tttttaggtt tagttaggtt atagattacc ctgaaaaata
    26281 acatttaact tattattaaa aacatgtaag aatagtttat aaatatttta ttttcgatgt
    26341 tgtttattat atccatttta tcgatggatg aggaatggct ctgccttccc tagttacaga
    26401 tgtagtaggg aaaccaattg cacaggcatc aaatccatat tcacctgttg gtactcctaa
    26461 aatatcacaa aaatctttat tatcgctcaa tcttcctatt acactaacaa gttcaaaacc
    26521 taatcctaaa gcagttgatt taagccacat attctgcatc acatgagata atgacttttg
    26581 ttctataggt ggtattccac gacgttctgc cacaataata agataaggtg ctgttcctat
    26641 accaagaacc cctttttcaa cttgcatttt taaggaagta atgaaggatt gaagggtaat
    26701 gttgttcttt gccttcttct cattgactga tatttgagtt ttaataacat caacaagaat
    26761 ggtttttatt ttattcattt gtaaactgtt atttgaaata acaaagaaat gtctgaaatc
    26821 tgtttgtttt gctactgaaa gtattgcata aggtgcaagt cttccagttt caattatttc
    26881 ttcaatcagc ccttttgaag gtacttcttt tgtaaaactt cttatggatc ttctagttct
    26941 taatagcata tccaaaactt cattaacatt gtttttatct aaattgttca tttttatcct
    27001 ccactaatag tattcttaag atgttctata atgcatccat ataaaggcga acttttcggt
    27061 catattaaac atttccttca aattaagtcg ctatttctat gaacacaatg ttgattaata
    27121 atctatatgt atattataat atttatattg cattatgcaa taaacaataa tatttaaact
    27181 gttaagtaat aaacatttac tataaaattg ttaattaaaa ttaaaaaaat ttatatggaa
    27241 tgaggaaata attcatgaaa aaagtatcta ttgatagaag aactatacgt actaaaaaaa
    27301 tgattaggtc tgcattagca gagttaatag atgagaaagg atttaacaat atatcagtta
    27361 ctgatttaac tcaaagggca gatataaata gaggaacctt ctatctgcat tatgttgata
    27421 agtatgactt gttagagagg attgaaaatg aaataataca ggaaattgaa gaggagacca
    27481 aacatcttga ttcatccaac atgatggaga tagatacctc aagtgaacct cttccattta
    27541 tggtaaagct ttttgaatat tttagaaaaa attcagtaat tattaaatct atcttgggtc
    27601 ccaaaggtga ccctactttt gaaagaaaaa taaaaaaatt tattgaaacc aatttgtttg
    27661 agaaacaaaa actaataaat tttaacatag atgaggcaat aatttcagaa gaatatttta
    27721 tacaatatat actttcagca gacttaggtg taattcaata ttggcttgaa aaaaatatga
    27781 aagagtcccc tgaagaaatg gcactgatac ttgctagaat gtcgttactc ggtccgttaa
    27841 gagcagtggg aattcgtaaa gattcataaa aatagtttta tatatattag actttgaaaa
    27901 tatatgttta aaaaccattt tttactacta ctgtttataa attaggggag atatcttaaa
    27961 atattagtaa aaggagtgct aagtgagtgt aataactatg agataagagg tttagttgat
    28021 atagtcaaaa cttcaaaaga atcaattaga aaagttaaaa tattgcaatt aaatttaaag
    28081 gagtaatata tgaaaaatga aaatataagt tatttggcgt taggattatg ttttggtgtt
    28141 gttttgggtt cattatttaa aaacttagct attggtttat cctttggatt agttattgga
    28201 gtagtattag acagtaagaa aaataaataa aacttcaata gtaacatata acaattatac
    28261 tatttttgta taaaatcatt ggaaatcaac aatagaaaat atttgttatt taaaaactaa
    28321 aaatacagtt taaacgacct aaaaatatat tatataatta ttataacaag ataagtgaga
    28381 gtaaggttat gataatagag taatattttt caattattac tctatttata tttatttagt
    28441 tatactatag tttatattgt tattttattt tcatatgcag tatagggaat ggattacctt
    28501 gttcatcgaa ctcagatttt ttaaagactt taaatcccat atgtttataa aagcctaatg
    28561 cttgttgatt ttgctcattc acatctacat aatttacatt taatacttct attgcatatt
    28621 ctacaagtct ttttccaatt ccatttccta tactataatt acttacaaat aacatttcaa
    28681 ttttaaaatc atgtattccc ataaaagctt ttataatacc tttttttgtc tcttacacat
    28741 aaaaaattac taacatattt agcacattct ttaacttgag gctttattga tattatattt
    28801 tcttcactta aaaagtcatg tgttgctctt actgatgatt cccgtgtatc tagtatttct
    28861 ttcatatctt tattgttaat aatatttaaa ttttcaattt ttatcattta ataattcctc
    28921 ttaagtaagt atgttactta atattatatg ttaaatatga tttaaaaagg ttataagaat
    28981 atttttgtat taaatataat caaataaata tatttatttg tcataaatta aatgtccact
    29041 aaaatagaaa aaataagaag aaataggtaa taaatgatag cttatagcaa aataaagatg
    29101 taagtataac aatagatgag attggaatta agtcactata cctaacagaa cattcttagg
    29161 cgaatataga agacttaaaa aagaaaagag aagtagacaa gaatttatta atgattacat
    29221 ataaaataaa tgaattataa ttttattaat gatataataa taattaacaa aaaataaaag
    29281 aggaacgagt taatgtttga tggcatacac tcattttcta accaataaaa caatagattc
    29341 aattaaggtt actaaagtct aatcaacaca taaacatata ggataaatac atctttttaa
    29401 tatattaatt atgttagact tacaaaaaat gtaaatacat agcttgttaa atcttgattg
    29461 aaccttaagt aataagggat aaaaaatgac ttgttcaaag gcaaagaata tactattaaa
    29521 tgatataagt aacttagaga taatatgtag taagtcaaaa gaactatata gcatcagtaa
    29581 gtaaaaagcg taaaaactta cataatgcac tataaaataa atgagttaga aaataaatat
    29641 aatttatata ataaagtagt acaaaaagga gtatttatgt ttggatatgt taaaataaac
    29701 aaaatggatt taacatttag agaatatgat tattacaaag cttactattg tggactatgt
    29761 aaatacttaa aaagaaatca tggagaaatc tctaggtttt ctttgaatta tgatataaca
    29821 tttttaattg ttttattaac agcagtatat aacccagagt ctataagtac agaggaagtg
    29881 tgtatagtaa atccatttaa aaagaaaaag gtaattacaa atgatattac agaatatgca
    29941 gcaagtatga atattctttt aacgtattat aaattggaag ataatttaat ggatgataaa
    30001 cgcataaaag ataaattggc atattatata tataaaaaca aattaaaatt agcatatgaa
    30061 aagtatcctg aaaaagcaga gtatataaaa caacaactaa atgaattaaa taagttagaa
    30121 aaagataaaa atataaatat agatgaagta tctagtatat ttggaaatat aatgggtgaa
    30181 gtatttgtct ataaaaaaga tgaaaatgag agaaacttaa gaatgatagg ttttaatata
    30241 ggtaagtata tatatctact agatgcctat gaagatttag atgaagattt taagaaagga
    30301 agatataatc catttataga gtatattgat aaaaatgatg agttaaaaga aaaagtaaaa
    30361 aaaataatag taacatcatt aggattttta gctaggggaa ttgataatct aaatctaaat
    30421 aaaaatgttg gaataataga aaatataatt tattctggtg tttatttaag atatattaat
    30481 atattagaaa gtagaggtgg aaagaatgta cagtaatact tataataatg aaaattttat
    30541 gagagcaaga gcattcatag aaaatagtga atttaaaaaa gcatatgatt ttttaaaaac
    30601 tttgactgac aaatgtgcag agtggtatta tttaactggc ttttctgcaa tgaacatagg
    30661 atattatgaa gaaggtgaag acttcttaaa aagagcaaaa tttatggagc ctgaaaatag
    30721 tgaatatagt gatgcactta gaagttatac acaatataga aatgattata gcaatagggc
    30781 tgacaattat aatagaagaa gacgtaatga tttagatgga tgttgttgct gttgctgtga
    30841 tgattgctgt tgttgtctag gtgacgattg ttgtgaaaat tgtgctaagc tttggtgttt
    30901 agattcatgt tgtgaatgtt ttggtggaga tttaataact tgttgttagg atgataaata
    30961 tgagtataaa agttgcttat ggaggagttt tattagcatt aaatgtgata ttgttgactc
    31021 ttacaaatat tattcctgta aatacattgt ttattatggg gttagcatca cttttagtat
    31081 caattgtaat aatggaatgg ggctttaaga gtggaatcgc tttttatata ggttctatag
    31141 ttttaggatt tatagtgatg gcaagtaagt ctcaatggat tgtatattca cttacctttg
    31201 gaatatatgg tattgtaaaa tatcttatag aaaaagatag gtctatatat attgagtatt
    31261 ttatgaaatt agtatttgca aatataatga tacttatatt gtatttttta ttaagaacaa
    31321 tagtttatat tccaataaac atatttatta taggttcatt tgagatagcc tttatagtat
    31381 atgactatgt ttacagttct tttataggtt attacaataa taggttaaga aagatgctat
    31441 ttaaaaaata atagttgatt tttatattta attttaatga aattgtagtt tggataagtg
    31501 taaatgatat atctgaatat acattaaagc ataaagctaa atttaatata ttcttagtgc
    31561 aacatataaa agctttgatt tttagtcaaa gcttttatat gttagatata aatattagat
    31621 ataatataag tttttaaaat agatagtata aattaaatat ccactatatt tacatctgta
    31681 gatagagaat aaataatatc ttttctttct actattatct caaca
//
LOCUS       DAEPOZ010000018        30247 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.018, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000018 DAEPOZ010000000
VERSION     DAEPOZ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30247
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.018"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            86..1144
                     /locus_tag="KR440_000691"
     CDS             86..1144
                     /locus_tag="KR440_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HBH0697754.1"
                     /translation="MIVCNNPGTWMRMYPQLRHAVWHGVTLADFAFPFFVISLGVTIP
                     ISINSKLKNNKSTLSIILSIFKRSILLILFGFFLNYLGNPDLNSVRILGVLQRMGLVY
                     FVTSLVYLLLKKLNVGSTATIITFLCIATFIIVGYYILAKPYGFELEGSLAQLVDLHF
                     FKGHLYKPEFEPDGFLTSIVAISSGMLGCTMGCVLLKENIGEYKKFFKILVMSIILLI
                     GAFIFNQYFPFNKRLWSSSFVLLMAGSYGMLLSIFYFICDIKNKSKIFTPIIALGSSP
                     IFTYMCLEILSHVFWNVPKLTNKVDYPTTLVEWTTYELITPWAGTTWDSLIFSLLYVL
                     FWVIVMSIMYKKKIFIKI"
     gene            1398..3422
                     /locus_tag="KR440_000692"
     CDS             1398..3422
                     /locus_tag="KR440_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH0697755.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHSTNLSEKTAGT
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGAGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            3642..4328
                     /locus_tag="KR440_000693"
     CDS             3642..4328
                     /locus_tag="KR440_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBH0697756.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            4423..5478
                     /locus_tag="KR440_000694"
     CDS             4423..5478
                     /locus_tag="KR440_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HBH0697757.1"
                     /translation="MIKKISTILSLVLLISISSTIEVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGIKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTAKLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            5593..7152
                     /gene="murJ"
                     /locus_tag="KR440_000695"
     CDS             5593..7152
                     /gene="murJ"
                     /locus_tag="KR440_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBH0697758.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDDKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRRR"
     gene            7194..8900
                     /locus_tag="KR440_000696"
     CDS             7194..8900
                     /locus_tag="KR440_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBH0697759.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNDTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            8922..9995
                     /locus_tag="KR440_000697"
     CDS             8922..9995
                     /locus_tag="KR440_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HBH0697760.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEYISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            10007..10729
                     /locus_tag="KR440_000698"
     CDS             10007..10729
                     /locus_tag="KR440_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBH0697761.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LICGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            10745..11902
                     /locus_tag="KR440_000699"
     CDS             10745..11902
                     /locus_tag="KR440_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HBH0697762.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNIDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRIILFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPIILTLFSVIIGTIFDKSTLLNRLLASRPKFWHVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            11905..12744
                     /locus_tag="KR440_000700"
     CDS             11905..12744
                     /locus_tag="KR440_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBH0697763.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNEDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYTFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            12759..13934
                     /locus_tag="KR440_000701"
     CDS             12759..13934
                     /locus_tag="KR440_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HBH0697764.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            13963..14712
                     /locus_tag="KR440_000702"
     CDS             13963..14712
                     /locus_tag="KR440_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBH0697765.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYKRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            14734..15642
                     /locus_tag="KR440_000703"
     CDS             14734..15642
                     /locus_tag="KR440_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBH0697766.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            15690..16442
                     /locus_tag="KR440_000704"
     CDS             15690..16442
                     /locus_tag="KR440_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBH0697767.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            16420..17763
                     /locus_tag="KR440_000705"
     CDS             16420..17763
                     /locus_tag="KR440_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HBH0697768.1"
                     /translation="MPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLAEVGNENVV
                     CVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDVDVILIAVG
                     TPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYINRNLKNNVK
                     VELASNPEFLSQGTAVKDTLYASRIVIGVESEWAREILEEIYKPFNQPIIVTNRNSAE
                     MIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLKAGIGYGGS
                     CFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQDMKVAVLGL
                     TFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYADSVDDAIE
                     DAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEYYSIGR"
     gene            17769..18860
                     /locus_tag="KR440_000706"
     CDS             17769..18860
                     /locus_tag="KR440_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBH0697769.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            18944..20857
                     /locus_tag="KR440_000707"
     CDS             18944..20857
                     /locus_tag="KR440_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBH0697770.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISVCIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNSELKKVAVAIIDDDKDKIGRIIHNVKIVGTTSDIKAVVEKYN
                     VDEIIFSIANIEKRRKKEIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            21056..21763
                     /locus_tag="KR440_000708"
     CDS             21056..21763
                     /locus_tag="KR440_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBH0697771.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYRWGATGPNAFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYARRLITATRVLQ"
     gene            22065..24176
                     /locus_tag="KR440_000709"
     CDS             22065..24176
                     /locus_tag="KR440_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HBH0697772.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDNWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTAL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(24374..25339)
                     /locus_tag="KR440_000710"
     CDS             complement(24374..25339)
                     /locus_tag="KR440_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBH0697773.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(25624..26580)
                     /locus_tag="KR440_000711"
     CDS             complement(25624..26580)
                     /locus_tag="KR440_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBH0697774.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVDIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            26948..27736
                     /locus_tag="KR440_000712"
     CDS             26948..27736
                     /locus_tag="KR440_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBH0697775.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVDDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(27859..28329)
                     /locus_tag="KR440_000713"
     CDS             complement(27859..28329)
                     /locus_tag="KR440_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HBH0697776.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            28468..29112
                     /gene="uppS"
                     /locus_tag="KR440_000714"
     CDS             28468..29112
                     /gene="uppS"
                     /locus_tag="KR440_000714"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HBH0697777.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            29155..30060
                     /locus_tag="KR440_000715"
     CDS             29155..30060
                     /locus_tag="KR440_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH0697778.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTSQQQKVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
BASE COUNT        11993 a         3299 c         5371 g         9584 t
ORIGIN      
        1 aatttcaaat aatgttgtag attctaaact tacgaatagt aggattaaat caatagatat
       61 aataagaggg ctatcaatag ccctcatgat agtctgcaat aatcctggga cttggatgag
      121 aatgtatcct caattaagac atgctgtatg gcatggtgta acacttgcgg attttgcatt
      181 tccttttttt gttatatctt taggtgttac aattccaata tcaataaata gtaaactaaa
      241 aaataataaa tccacactga gtataatact tagtatattc aagagaagta tcttattgat
      301 attatttgga tttttcttaa attacctagg aaatcctgat ttaaattctg tgagaatact
      361 tggagtactt caacgtatgg gattagtcta ttttgtgact agtttagtat atttactact
      421 aaaaaaatta aatgttggaa gtactgctac aataattact tttctatgta tagctacatt
      481 tataatagtt gggtactaca tattagcaaa accttatgga tttgagctag aaggttctct
      541 tgcacagtta gtggatttac atttcttcaa gggacatttg tacaagccag aatttgaacc
      601 agatggtttt ttaactagta tagtagcaat ttcttcaggt atgttgggat gcactatggg
      661 atgtgtactt ttaaaagaaa acataggaga atataaaaaa ttctttaaaa tactggttat
      721 gagcataatc ctgctaatag gtgcttttat ctttaatcag tattttccat ttaataaaag
      781 attatggtct agctcatttg tacttttaat ggctggcagt tatggtatgt tactgtcaat
      841 attttatttt atatgtgaca tcaaaaataa aagtaaaata tttaccccaa taatagcact
      901 tggaagtagc cctattttta catatatgtg ccttgagata ttgagtcatg ttttttggaa
      961 tgttccaaaa cttacgaaca aagtcgatta tccaactacc ttggtagagt ggactacata
     1021 tgaacttata acaccttggg caggtacaac ttgggattct cttatatttt ctttattgta
     1081 tgtcctattc tgggttatag taatgtctat aatgtacaag aaaaaaatat ttattaaaat
     1141 ttagtgcaat atttttagat gataaagtct ttgatttgtt caaatatacg gaaactatca
     1201 aatataatca aaatttctcc tcgataaaat ttaaaaacca tgccataaaa gattacaaaa
     1261 agtttacaat ttgtaatttt ttgtgctata atgaaaaaag atttacataa tattgaattt
     1321 atgtaatata aaacaagctt aagatgtatt aaaaaagatt taaagattta aagaaccaaa
     1381 aaattagggg ggatatgatg aaaaaaacaa caaaattatt ggcaacagga atgttgtctg
     1441 tagcaatggt ggcaccaaac gtagctttag cggctgaaaa tactactgca aatacagaat
     1501 ctaattcaga tattaatata aatttacaaa gaaaaagtgt agtgttagga agtaaatcta
     1561 atgcaagtgt aaaatttaaa gaaaaattaa atgctgattc aataacatta aattttatgt
     1621 gttatgatat gccacttgaa gctactttaa attacaatga aaagacagat tcatatgaag
     1681 gtgtaataaa ctataacaaa gacccagaat acttaaatgt atgggaactt caaagcataa
     1741 aaataaatgg taaagatgaa cagaaagttt taaacaaaga agacttagag tctatgggat
     1801 taaatcttaa ggattatgat gttacacaag agtttataat aagtgatgca aattcaacaa
     1861 aagcagtaaa tgaatatatg agaaaaactt ctgcgccagt aaaaaaactt gctggagcta
     1921 ctagatttga aactgctgtt gagataagta aacaaggttg gaaagatggg tctagtaagg
     1981 ttgtaatagt taatggagaa ttagcagcag atggaattac agctactcca cttgcatcta
     2041 catatgatgc accaatatta cttgcaaata aagatgatat accagaaagt acaaaagcag
     2101 aattaaaacg tctaaatcca agtgatgtaa taattatagg tgatgatggt tcagtttcac
     2161 aaaaggcagt atctcaaatt aaatctgctg taaatgtaaa tgtaactcgt attggtggag
     2221 ttgatagaca tgaaacatct ttacttatag caaaagaaat agataagtat catgatgtta
     2281 ataaaatata catagcaaat ggatatgctg gtgaatatga tgctcttaat atttcatcaa
     2341 aagctggtga agaccaacaa cctataatat tagctaataa agactctgta ccacaaggta
     2401 catataattg gttatcttca caaggtttag aagaagctta ttacataggt ggtagtcaaa
     2461 gtttatcatc aaaaattata gaccaaatat ctaaaatagc aaaaaatggt acttcaaaaa
     2521 atagagtttc tggagcagat agacatgaaa caaatgcaaa tgtaattaag actttctatc
     2581 cagataaaga gttgagtgca atgttagttg caaaatctga cataatagtt gactcaataa
     2641 cagcaggacc tttagcagct aaattaaaag ctccaatact tataactcca aagacttatg
     2701 tttctgcata ccatagcaca aacttatcag aaaaaacagc aggaacagta tatcaaattg
     2761 gtgatgggat gaaagatagt gttataaatt ctatagcatc tagtttatca aaacacaatg
     2821 caccaacaga accagataat tcgggttcag cagcaggaaa aactgtagtg atagacccag
     2881 gtcatggggg ttctgattca ggggcaacaa gtggattaaa tggtggagca caagaaaaga
     2941 aatatactct taatacagca ttagcaacta ctgaatattt acgttcaaaa ggtataaatg
     3001 ttgttatgac tagagataca gacaaaacaa tggctttagg agaaagaaca gcattatcaa
     3061 acactataaa gccagattta tttacaagta tacattacaa tgcgtctaat ggagctggta
     3121 atggtgtgga aatttattac aaagtaaaag ataaaaatgg tggaacaact aagactgcag
     3181 catcaaatat cttaaaaaga atactagaaa aatttaacat gaaaaataga ggaatcaaga
     3241 caagaacact tgataatgga aaagattacc tatatgtgtt aagaaataac aattatccag
     3301 caatacttgt tgaatgtgca tttattgaca ataagagcga tatggataag ttaaatactg
     3361 cagaaaaagt aaaaacaatg ggaactcaaa ttggaatagg aatagaagat acagtaaaat
     3421 aaaataaaaa atattaaagc aaaacatatc atctcctgtt ataatgtaaa atataacaag
     3481 agatgatata tttttgtaaa gaatcaaatt gtttacaatg atgtttcttg tattgataat
     3541 ctgctcatta ttgagtataa tatagaaata agtcaaaaat aagatagaaa taagttgaaa
     3601 ttaagaatta aaaaaatttt tattccaata tggaggtaac tatgtctgga tatactaatg
     3661 atgagtgtga aatacctaaa attaaaagct atccaggggc agataaagaa attgcaagtg
     3721 agattgacta tagcatagta aaaggaacgg ttttattcga cttgtaccaa agaattatgg
     3781 atttagtttt atctataata ggacttgtga taggtttacc tttaatagca atatttggaa
     3841 ttcttattaa aatagaggac aaaggaccaa taacttataa gcaagaaaga ctaggaaaat
     3901 gcgggagaag attctatata tataaattaa gatctatgag aacagacgca gaaaaatttg
     3961 gcgcacaatg ggctgaaaaa gatgatccta gaataactaa ggttggaaag tttattagga
     4021 aaactagaat tgatgagatt ccacagctgt ttaatatatt aaagggggat atgggcttaa
     4081 taggccctag accagagaga ccgaacttta ctgttcaatt caatgaggaa atacctggat
     4141 ttatcaacag gctcgctata aaacctgggc ttacaggttg ggcacaagta aatggtggat
     4201 acgaaatcac tcctgaagaa aaacttaaag aagatattta ctatataaaa aataggagta
     4261 tattacttga ctttaagata ctctttaaaa ctgttaaagt agtattgaca ggagacggag
     4321 ctagataatt ttaaaatatt agattactaa tcaatgaaaa agctctttaa aaagagcttt
     4381 tttattgtca aaaaatacaa aaaagaaaag gaatgagaac ttatgattaa aaaaatttct
     4441 actatattat cgttagtttt gttaatctct atttctagca caatagaggt atttgcagat
     4501 gcaaatccca aaagagaatt gatagaaggg agtataccag agatttcgac agaattaaat
     4561 aaaagagcat ttaaggattc taaagaagta attcttgtaa atgaagagtc aattgtagat
     4621 agtataagtg caacaccttt agcatatgca aaaaatgctc caatagttgt aactaagagt
     4681 aaaaatcttg gtagagtaac aagaaattat ctaaaagaac taggtccaga gaaggttacg
     4741 atagtaggtg gtttaaaggc agtatctaaa gatgctgaac gtaatataga aaaaatggga
     4801 ataaaagtag aaagaataag aggaaaagac agatatgata catctttaaa gatagctaga
     4861 gaaatgtata gaacagtagg atttgatgaa gcatttttac ttagctcaac aacaggatta
     4921 gagaatgcca tatctgtata ttcttatgcc gctaaaagtg gaatgccaat aatatgggct
     4981 aaagatgaag ggtttgagga acaaattgat ttcttaaaag gtaagaatct taaaaaaata
     5041 tatgcattag gtgattcaaa agaatttatt gcagagattg attctaactt gaaaaatata
     5101 gaaggaataa agcaaataaa taagtcaagt acaaatgttg atttaataaa gaagttttat
     5161 gatgaaaaag atataaagaa aatctatact gctaaattag actttggtag cagatctgat
     5221 gtaaacgaat atatttcttt gggagtagta tcagcaaaag aaaatatgcc aatactgatt
     5281 tgtagtgata acttaagtcg tgcacaagat aaattcttga aagatagcaa tataaatgat
     5341 gtagttgaag ttgggtatac agtaggtgat tatagtctat ttaagagcat atttaatcta
     5401 acattcttat cttgtatagt attaatacta ttattactat tgattacatt tagggcactt
     5461 agatatgagt cgaaatagtt tttaatttat aaggaagctt ttcataggtt aaatttactt
     5521 aattagaata atgtttataa gcagttttaa aagttctact tttagagttt aaattagata
     5581 aggagaaaga agatgtcaaa aacagctaaa gcagcattgt ggattatggc agccactatg
     5641 ttttcaaaag tattaggttt ccttagagaa cttgtacttg caaatttcta tgggacaggt
     5701 atgtatgcag acgtatttgt attaacatta aatatacctg gtttaatcat agcagtcata
     5761 ggctctgcag ttgctacaac ttatatacct atgtactttg agacaaagaa aagactcgga
     5821 gatgaaggtg ccttaaaatt tactaataat gttttaaata tatgctatat aatggctata
     5881 gtaatagcta ttattgggct tttatttaca gagcaatttg ttacagtatt tgcagcagga
     5941 tttagaaacg accctgctaa gttccaagca gcaatattat ttactaaaat aatgatttca
     6001 ggagttttat tcctaagtgg aagtaagata tttagttcgt ttttacaggt gaatgacagt
     6061 tttgtaattc ctgggcttat aggaatccca tataatatta taataatagc agcaatagca
     6121 ctaagtgcag gtaaaaacgt atggatacta ccagcaggag cactattggc tatggcgagt
     6181 caactattat ttcaactgcc atttgcattt aagaagtcct acaagtataa gccatatata
     6241 aacttaaagg atgagtctat aaaagaatta gtaaacctag tattgcctat gttggtagga
     6301 gtagctgttg gacagttaaa tatatttgtt gacagattat tggcaactac tttgggtgat
     6361 ggaaagttgt ctgctcttaa ctatgcaaat aggctaaatg agtttgttat ggcattattt
     6421 gttacatcta taataacagt tatatatcct aagctagcaa aaatgtctgg taaagatgat
     6481 aaagaaggat ttataagtac tattgtaaaa tcttcaaatt gtattatatt ggtggtgctt
     6541 cctatatcta taggagctat tatattagca gagcctcttg ttaggatatt atttcaaagg
     6601 ggtaaatttg atgcgttatc aacagatctt acatctatag cgcttagatt atattcacta
     6661 ggtttattgg cttgtggagt aagagatgtc ttatataggg cattttactc tctttcagat
     6721 acaaaaacgc caatgataaa tggaagtata gctttaataa taaatatagt tcttaattta
     6781 atattgataa gaccattagg tcatgcaggt attgctatat caactagtac atccaatata
     6841 attacagtta tattgttatt tatatctctt aaaaagaaaa atggatattt tggaggggat
     6901 aaaattataa agactggtct aaaaagtttg gtagcatctg gtgtgatggc agtagctact
     6961 ttattgatat ataataatct atatgcattt atgggttcag gaacaattaa agaaattata
     7021 tcagttggag ctggagtatt aggtggagct tctgtatata ctgtattgat agttttattt
     7081 aaggtagaag agatggactt agcttttgaa tttttaagaa aaggaaaaca aaaactactt
     7141 agaagaagat aattaccttg aaattttaaa ttagagtcag aaaggatgaa aatatggatt
     7201 ataagaataa ttatgagatg tggttaaatt ctccttattt tgatgagcaa acaaaaaatg
     7261 agctattaag cataaaagat gatgaaaaag aaatacaaga tagattctat aagaatttag
     7321 agtttggaac aggtggttta agaggtataa taggagcggg aactaataga atcaatattt
     7381 atactgttag acgtgcgaca ctgggcgttt taaattatat catgaagact caaggagaag
     7441 aaggtaaaca aaagggaata gttatagcac atgatagtag atacatgtct agggagtttt
     7501 gtatagaagt agcaaagact ttaagtgctt atggtgtaaa ggcttatatt tttgaagaat
     7561 taaagcctac accagaactt tcttttgctg ttagatattt aaagtgtgct atgggaatag
     7621 taataacagc aagtcataat cctaaagaat acaatggata taaagtatat gactcagatg
     7681 gtggtcaaat ctgtatagat atggcaaatg atataatagc agaagttaat aagattgatg
     7741 attacagcac tattaaaagt atagattttg aagaagcttt atctaaaaat ttaataacta
     7801 tattagataa tgaagtagat gatgagttta taaaagctgt taagaagcaa gttttaagac
     7861 aaaatataat agatgaatac gggaaaaaat taaaaataat atatacacct attcatggta
     7921 caggaaataa accagtaaga aaagtcttaa atgaatgtgg ttttgaaaat gtaatggtag
     7981 ttaaggagca agaattacca gattctaatt tttcaacagt aaagtatcct aatcctgaag
     8041 aaaagtcagt gtttaatata gctattgaaa tggcaaagaa taatgacact gatttgataa
     8101 taggaacaga ccctgattgt gatagagtag gtatcgttgt aaaagattca agtggtgaat
     8161 atgtagtctt aaatggaaat caagtcggtt cacttttagt tagatatata ttagagagtt
     8221 tagttgaaga aaataaactt cctaaaaaca atcctacaat aataaaaact atagttacat
     8281 cagaactagg tgcaaaaata gcaaaggctt ataatgtaga ttgtttaaat actttaacag
     8341 gatttaaatt tataggagaa aaaataaaag cgtttgaaga aagcaatgat agaagtttta
     8401 taatgggata tgaggaaagc tatggttact taattggaac tcatgctaga gataaagatg
     8461 gtgttgtatc ttcacttatg atatgtgaaa tggcagcata ctattcttca aaaggtatga
     8521 acttgtatga agctttgata gatacatata ataaatttgg atactacaaa gaggacttaa
     8581 agtcagtaac tttaaaaggt atagatggaa taaaaaaaat aaaagagatg atgctttact
     8641 ttagaagtgt taaaatagat aatgtggctg atgtaaaagt agataaaatt ttagactata
     8701 aagatggtgt tgatgattta cctaagtcag atgtacttaa atttttactt gaagatggct
     8761 cttggatagc tataagacct tcaggtacag aacctaaaat taaattttac tttggagcaa
     8821 attcagacaa tcaagaagat gtagaattta agttgaacaa tttaatatct tatatattaa
     8881 atgtagtgga ttctatataa aaagctaagg ggagataaat aatgaaagta tacaatgtca
     8941 taatggctgg aggaggagga actcgtttct ggccacttag tagacaagaa gtacctaaac
     9001 agcttataaa tttaagtgga gaagatgctt taataaacga aactataaac agaatagatt
     9061 ctctagcaaa aaaagatgat ttatttatag taaccaatga gaagcaattg gaagctttaa
     9121 aggatatagt aaaagataaa tgtttggata gtaatatatt gccagagcca tgtgccagaa
     9181 atacagcagc agctataggc tttgcagcat ttaacataat gaaaaaatat ggtgatggtg
     9241 ttatgtgtgt atatccagct gaccattata taaaagatga aaaagaattt aaatctattt
     9301 tggaaaaagc tatctatata gcagaaaata atgataaact agtaactatt gggataactc
     9361 ctacatttcc atcaacagga tatggataca taaattttaa tagagaaaat acgatagaag
     9421 atgttgcata tgaggtagtg gagtttgtag aaaaaccaaa ctatgaaatt gctaaagaat
     9481 atgtaaactc taaaaagtat gtatggaata gtggaatgtt cgtatggaag gtatctaaaa
     9541 tattagaaga ttttaagaga tacttgccaa aagtatatga aaaattagaa tatataagta
     9601 aatacttagg aacaaaagaa gaaatggaaa aaataaaaga aatataccca actatacaat
     9661 caatctctat agattatggt attatggaaa gaagtaatga tgttatagta gttcctggtg
     9721 attttggatg gaatgatgtt ggttcttggg attctctagg tgcaatatat ccaactgatg
     9781 atgaaggtaa catcaaaaga ggtgaaaata ttactataga tactaaaaat tcaattatat
     9841 attcagatga caaattaata tcaacaatag gtattagtga tttgatagta gtatctacga
     9901 atgatgcagt tatggtttgt agaaaagata aagctcaaga tgtcaaaaaa atagtagaac
     9961 agttaaaaga agaagataga caagaatata tgtaggaggt gttcatttga ttcctaaaaa
    10021 aatacattat gtatggtttg gtggtccaaa aggaaatata gaaaatatat gtataaattc
    10081 ttggaaagaa aagcttccag aatatgagat agtagagtgg aatgaaaaaa actttgatat
    10141 agagaaagaa ataaaaggaa ataagttttt ggaagaatgt tataaaagaa aactttgggc
    10201 attcatatct gattatacaa gaataaaagt actctatgaa caaggtggag tttatatgga
    10261 tacagatatg caaatattaa aggatataac tcctctttta gaaaataata ggctaatctg
    10321 tgggtatgaa gatgatagag agtatataaa tggtgctata ataggtgtag aaaaaggaca
    10381 tcctttttta aaagacttat tggagtatta tgaaaaagaa gtacttactt cttcattgtt
    10441 tacaatacca aaaataatga ctcatcttat ggaaaagaat tataagaaga ttgacccaaa
    10501 taattatgaa gaaggaatac gtgtttacga caaagaatat ttttatcctt ttggatttaa
    10561 agaagacttt acaccagaat gtataacaga aaacactttt gggatacatt ggtggggaaa
    10621 aagttgggct aaaaagagaa attatttctt agaaagcaaa cacttaacag gtgtaaataa
    10681 gatatggaag tgttgtaaaa tatttgcaag taatacttta cgaagctaaa agggagaaag
    10741 taaaatgaag actttaaatg atgatagaaa tattatgatt agaaaatatc tttatatatt
    10801 tgttttgttt atcatattac ttagaaagta tatgatatca tttattgacc caaatataga
    10861 tatagggatg attaaatcag cgttatttta ttcttcaata ggaatactaa tgctactttt
    10921 tttatttgat aagagaaaaa gtataacaga aatggtacta gttggagttt gtgtcttact
    10981 ttatttatta aatagggaag gagctatatt attaatcgtt ttattagctg tatcagctaa
    11041 gcaaatagat gataaattta tagttaaaaa ttatttaata atatcggcat gttttttaat
    11101 ggtagctata ttattattta atttatttcc ttcattaata tttaatcagg aagtccacta
    11161 tagatatata gaaaaaatag atatgcttgt tacaagaatg gattttggac ttggtaatcc
    11221 aaatagtgta ttttatcata tggttactat atatgcagca tatatattct taagatataa
    11281 agattataat aaatgggata gaataatttt atttggttct gcattttttg tttatcaaac
    11341 cacatatagt agaactggat ttttcactat attagcagga ttaatatttg tagaaataat
    11401 aagatggata gatataaaaa aaataaaagg actgccaatg ctcctaaaga cattaccaat
    11461 aatacttact ctatttagtg taattattgg tacaatattt gataaaagca ctttattaaa
    11521 tagattatta gcaagtagac ctaagttctg gcatgtatac ttggctgaaa aggggaattt
    11581 tctaaaacct tttggaaatt catatagtcc tgctataaaa gcaactaatc cattagatag
    11641 ttcttatgta tatataatta gtgtactagg agtagttgct tgtatattat ttatgtattt
    11701 gatgtataaa ggaatagaaa gctttataga aaaagataaa aaagcatatt tagtagcttt
    11761 atttatattt ttactatact cttttgcaga aaatttatta ttagaagctt catttagttt
    11821 tgcagtagta ttactaataa aagaagtgat attaaatgat aaaagggaaa tagatttgtg
    11881 gaagatgaaa agtaggaggt agtaatgtta atatcattaa taatgccaac attaaataga
    11941 tatgatgata tatacttact tatggatagc ttagaaaatc aaacttacaa aaactttgaa
    12001 cttatagtag tagaccaaaa tgataatagc aaagttaaag aaatagttga taaatacatt
    12061 gataagttag atataaagta tataaaaagc tctaaaaaag gattgagtta taacagaaat
    12121 gtaggcatag ataatgctgt agggcaaatt ataggtttcc ctgatgatga ttgtgtctac
    12181 gaaaatgata cattagaaaa agttataaat ttctttaata aaaatgaaga ttataaaata
    12241 tacagttgca aaactatgga ttcaaataaa gttgatgcat ttaagaaaat gtatgatgga
    12301 acttgtgata tcacaagttc caacgttttg gatactataa catccataac tttttttatt
    12361 gattttgaag gtaaagatta tacaagattt gatgaaaaat tgggtgttgg tggagagttt
    12421 ggtgctggtg aagagataga ttatgtttta aacttattga gtttaggttt taaaggaaaa
    12481 tactttggaa atgatataat ttaccatcct gcaaaaaaac attctaaatc taaagaaaag
    12541 tatcaaaaag attataatta tggaagaggc tttggagcgc tttgcaaaaa ggaaatagtc
    12601 tatagaaaaa attatacgtt tgcaaaagtt atggtatcaa aacttgtaag aaatataggg
    12661 ggtcttatat tgagttccaa tagagattat catagtgcca ctataaaagg aagaattaat
    12721 ggatttagac agtacaagtt ataatgagga tggtgtttat ggacaaggtt aaatttttaa
    12781 taaatatgct tttggctttt tttatatatc catttaataa gcataagttt aatggtagag
    12841 atatctggct tataggtgga cattcagggg atatatacaa tgataattca aagttttttt
    12901 atgagtatat gctaaaagaa cataatgatg tagaaactta ttgggtagta aataaagaca
    12961 gtaaagtatt tgataaaatt ccaggcaaaa aattaataag aggaagtgta gaaaactatc
    13021 tgtattatta caattcaaaa gctatagtat tttcacatgc accatcagca gatatagcac
    13081 catacaattt tgctgtacct gttcttaatt attttcataa aaaaacgata aaagtgtttt
    13141 taaatcatgg gactataagc tttaaaaaaa gaaaacctat gaacaaaaag ttcaaaaata
    13201 ttatagataa cctatataaa agctataata tagttacagc aagttctgaa tttgaaagaa
    13261 atgttatggt aaatgattgg ggaatgttag atgactcagt ttatataatt gggaatgcaa
    13321 gatatgacaa tttaccaaca aatgaagttg cacaaactcg agatatatta tatactccta
    13381 catggagaga ttggataaaa tttagttcag gaaaatttac ggatacagat tattttaaaa
    13441 atataatgaa ttttttaaat gatgacaaat taaataaaat attagatgag aaagatataa
    13501 atgtaaaaat ttatatgcat catcttatgc atgagtttat agatgatata aaagaaaata
    13561 taacaggaaa acgaattgtg tttttagata aaggggtaac tctagcaaat gaaatcagaa
    13621 aatcagctgc aaatataact gattattcaa gtgtagccat agactttttg tatatgaata
    13681 gacctatctt gttttaccaa ttcgatttgg atgaatatat ggaaaaagtt gattcatata
    13741 tagatttaaa aagtgaaatg tttgggtctt tggcttataa taatgatgaa gccgtgaata
    13801 aacttattga tattattgaa aataattttg aggttatgga taaccaaaaa aatgaaagaa
    13861 ataagttttt cagatataat gataataaga actgcaagag gatatatgat tgtgtattaa
    13921 gtaaaattaa ataaatattt tataaatgat gaggaaggta atatgaagaa gaatttagta
    13981 tctataatta ctcccatgta taattctgaa aaatttattg aagcaaccat aaaatcagta
    14041 ttaaaccaaa cttatcaaga atgggaaatg ttaattattg atgattgctc aacagataat
    14101 agtcctaata tagtcaaatc ttatatgcaa caggatagta gaataaaatg tataaagact
    14161 gagactaata agggtgtctc taatgctaga aatttagcac taagtaaggc aacaggacaa
    14221 tttatagctt ttttagatag tgatgaccaa tggaatagca gtaagttaga aaaacaagta
    14281 aattttatgt tagaaaatga ctatgtaatt tcatttactt catatgaact gatggatgaa
    14341 aatgataaaa aattaaacaa agtaataaaa gttccaccta atgtagatta taaaagatta
    14401 ttaaaaggaa atatcttagg atgtcttact gttgtgattg ataagtctaa attggatttt
    14461 gaaataagaa tgagtggtgt aagacatgaa gactatgttt tatggttatc gatattgaaa
    14521 aaagggcata ttgctcatgg aataaatgaa gttctagcat tatatagaaa atctagcaat
    14581 tccctaagtg gtaataaaat aaaagctgct atgtggactt ggaatatata tagaaatata
    14641 gaaaaaatac ctttatacaa agctatatac tactttatta attatggaat aaacggaatc
    14701 aaaaaaagtt agacagagag gaagatttaa ttaatgtata aatataaatt tactgtattt
    14761 actccgacgt ataacagagc tcatttactt gaaaatttgt acaatgattt aaaagctcaa
    14821 acttatgatt tcaatgattt tgaatggttg attgttgatg atggaagtag cgacagtact
    14881 aaagagttag ttgaaaaatt tatatcagaa aataaattaa atataagata tatatacaaa
    14941 gaaaatggag gaaaacatac tgcaataaat ctaggtgtaa aaaatgcaga tggagagcta
    15001 tttttcattg tagatagtga tgatggttta attaaagatt ctcttgaaat tgctaatgat
    15061 gagtgggatt ctttagagaa taaagaagga tttagtggag ttgttggact ttgtgtatat
    15121 ccaagtggag atttgatagg gacagaaatg cctgaagata agaaaatatg tcactttgca
    15181 gacctatatt ttaagtatgg cgttaaggga gataaaagca tagtatttgt aactgatgag
    15241 cttataaaat ttccttttcc agaaagaaaa gaagtgagat ttttaccaga aagtgtagta
    15301 tggaatgaaa tgtctaaata ctataaagtt atatgtgtaa ataaacctat gataataaga
    15361 gattatttgg atgatggttt gactaagaat atattaagta aaaatgcatt aagaggtaga
    15421 gcattagagt ttttatattt aataaatcaa aatacatatc cattaagtag atatccatat
    15481 atgtggataa aaaattacat aaatttagcc agatactcat tgttgtctga tagtcattat
    15541 tttggtgaat taagaaaagt atctgataaa ttgttgtatt tagcattatt cccattagga
    15601 tattacaaat atattggaca aagaaaatta gtatctaagt aaacttgttg gtatagtact
    15661 gctagttgct atataaggag agtaaaaata tgaatgagcc tttagtatcg ataattactc
    15721 ctgtttataa ttcagaagag tttttatcag agacgataaa gtcaatccaa aatcaaactt
    15781 ataaaaattg gcagctttta ttagtggatg attgttctaa ggacaattca agtgaaatta
    15841 taaaaagttt tcgaaaagaa gatgctagaa taaaatatat aaagcttgaa aaaaattctg
    15901 gagctgcagt atctagaaat gttggtatta agaatgcgga aggtcgattt attgcatttg
    15961 tagatagtga tgatttatgg gatagtagaa aattagagat tcagatagaa tatatgttaa
    16021 aagaaaatgt aggtttttct tttacatctt atagatatat gagacaagat gggtcaaaaa
    16081 cgaataaggt tgcaagagct cctaagaaaa ttgactatga aggattactt agaaatacta
    16141 taataggatg ttctacagtg gtcattgata aggagattgt aggagagttc agcatgcctt
    16201 tggtaagaag aggacaagat actgcgacct ggttacagtt acttaaaaag gaaaaatatg
    16261 catatgggat tcaggaggat ttagtgaatt acagattagt tggaaattct atatcaagta
    16321 acaagataaa agcattaaaa cgaacttgga atacttatag aaatgttgaa aatttaagtt
    16381 taccaaaaag tctatatgta ttttgtttct atgtttttaa tgccattaaa aaaagagtat
    16441 aagttttata tacttggagg taaaaaaatg aaaatagcta tagctgggac gggatatgta
    16501 ggtttggtta caggtgtttg tctagcagag gtaggcaatg aaaatgtagt ctgtgttgat
    16561 gtagatgaac aaaaagttaa atcaatgaaa cttggaatag cacctatata tgaagaaggc
    16621 ctagaagaac ttatggttaa aaattacaac agaggaagaa tagattacac tacagactat
    16681 aaaaaagctt atgaagatgt agatgtaata ttaatagctg taggtacacc tgagagaagt
    16741 gatggctcag ccaacctaga ttatataaag gcagttgcaa agcaaatagc tgaaactgtg
    16801 acaaaagata ctctagtagt aataaaatct acagttccaa ttggtacaaa tgaagagata
    16861 gaagattata taaatagaaa cctaaaaaat aatgtaaaag tagagcttgc atctaatcca
    16921 gagtttttat cacaaggaac agcagtaaaa gatactctat atgcttcaag aatcgttata
    16981 ggtgtagaaa gtgaatgggc tagagaaata ttagaagaaa tctataagcc ttttaatcaa
    17041 cctataatag ttacaaatag aaatagtgct gagatgataa aatacgcatc aaataacttc
    17101 ttagctctta aaatatcatt tatgaatgat attgcaaatc tttgtgaaat agcaggagct
    17161 aacatagaag atgtagcttt aggaatgagt tatgatgata gaataggaag taagttttta
    17221 aaggcgggaa tagggtatgg cggttcttgc tttccaaaag acacaaaagc tcttcactgg
    17281 ttatctgaac ataatggtta tgaattaaaa acagtaaaag ctgcaataga agttaatgaa
    17341 agtcaaaaac ttaaattagt tagaaaggca tctaagttag tagagagctt tcaagatatg
    17401 aaagttgctg ttttaggttt gacttttaaa ccagacactg atgatataag agaagcacca
    17461 tctataccaa atataaaata tttgcttgaa agaggggcaa gagttactgt atacgaccca
    17521 attggtgtaa ataatactaa gaaaatattt ggtgactcta tactttatgc tgattctgta
    17581 gatgatgcga tagaagatgc agagatatgt tttataatga cagaatggaa agatgtatta
    17641 aattatgata tacataagta taaggaaaag atgaaaaatc ctttagtcct tgatggaaga
    17701 aattgttata aaattaaaga tatggaagag ttaggaattg agtattattc gataggaagg
    17761 taaatattat gaagactgtt tgttatttag ctgatgggtc aaatcctcat actttaaaat
    17821 ggtgtagttt ttttaaaaat aaaggatatg acattcatgt aatctcttta aatggaggtc
    17881 acatggaagg aataaaaatt tataattttg gttctaatgt agaagaatta aaaaatgaaa
    17941 atatttctaa aaaaacggga tacttaggtt caataaagca gataaagaag ctagtaaatg
    18001 aaataaagcc agatatattg catgcacagt atgcaagtag ttatggattt ataggaagtc
    18061 tacttgggta tcatccatat attatttctg tttggggtac tgatatttat gatttcccta
    18121 ataatggttt tatacaaaaa caaattataa aacataactt tagaaaagca gactatatct
    18181 tttcaaatag tagagatatg gctaaagagg ctaataaata tacagctaaa catgtagatg
    18241 taaccttttt tggagtagat atggataggt ttaagcctat ggaagtagaa aaagaagacg
    18301 cttttgtaat aggaattata aagagtttag aaaaaaaata tggaatagaa tatttaatac
    18361 aagcatttaa gatgcttaaa gatgaatata aagataagaa aataattctt aaaataggtg
    18421 gctctggaag tcagatggac aacctaataa atttagctaa agaattgggt atagaaaatg
    18481 atgttcagtt tttaggaaga atatctccag aaaatgtaag caagactttt aattcttttg
    18541 atgtaactgt atttccttct ttaagagagg gatttggagt ggcagctata gaatcagaag
    18601 catgtgaagt acctgttata gttacaaatg taggaggaca tccagaatct gtatgggaaa
    18661 atgaaactgg cttaattgta gaaccaaaac aaccagaaga aataaaaaat gctataataa
    18721 aactaatgga aaatgatgaa cttagactta atatgggtaa gaaagggcgt caatttgtta
    18781 gagaaaatta tgaggttaat ttaaacttta atgatataga aaaaatttat gattctatat
    18841 ttgataaata taaaaaataa aatgaaaaaa tacagcaaaa gatagatgtt gttggctatg
    18901 cttaggtgcg tagcttttgc tgtttaaagg agggcttcta gttatgcgag aaagtataga
    18961 tttagagaaa gaaagctttt ttaataggct ttttaaaaaa tataaattta gacatttaac
    19021 actattaatt ctagatattt gtagcgtact agtagcgttt cgaatgtctt taagcttgac
    19081 aggtaattta aatgcattta ccactaatga tataataatt tcagtgtgta tatatatagt
    19141 acttcatata gtatcattta gattgtttaa gtgctacaac acattatgga gatatgcagg
    19201 agaagaagaa attatatcta tttttgtggc aactttagct tatttaattc cgatttatat
    19261 aataaataaa ttattagggt ttgattatcc tataatgttt tatgtattaa atacaatatt
    19321 tattataatg tttacaagtg gcgcaagaat agcttataga gctataagaa tagtcatgaa
    19381 caagacatat tctagaggaa aggtttcaaa tatccttata attggtgcag gagatgcagg
    19441 agaaatggtt atacaggaac taaaaagaaa ttcagaactt aaaaaagtag cagtggctat
    19501 aattgatgat gataaagata aaattggaag aataatccat aatgtgaaaa ttgtaggaac
    19561 aacttcggat ataaaagctg tggtagaaaa gtacaatgta gatgagataa tcttttcaat
    19621 agcaaacata gagaaacgta gaaaaaaaga gattattgat atatgtaaaa acactaactg
    19681 taaaataaag acaataccag gaatttacga aattatagat ggaaaagttg atataaaaca
    19741 gattagagaa gtagagatag aagacttgtt gggtagagag cctataaaaa caaatttaag
    19801 agagataagt aattacattg agggaaaagt aatcctaata actggaggag gaggctcaat
    19861 tggttcagag ctttgcagac agattgcagg atttaatcct aaagagctta ttatagttga
    19921 taattatgaa aataatgctt actcaataca acaagaatta cttagaaaat ataaaaataa
    19981 attagattta aagacagtaa tagcatcaat aagagaagaa aaaagaatgg atgaaatatt
    20041 taataaatat aaaccagaag tggtatttca tgcagcagca cataaacatg ttcctcttat
    20101 ggaatcgagc cctggcgaag ctataaagaa taatatattt ggaactttaa acatagctgg
    20161 cttgtcttca aaatatagag ctaagaaatt tgtccttata tctacagata aggctgtaaa
    20221 ccctactaac ataatgggag ctactaaaag agctgctgaa atgattatac agactatgaa
    20281 tgctgaaagc cagactgaat ttgttgctgt tagatttgga aatgtgcttg gtagtaatgg
    20341 tagtgtaatt ccacttttta agaagcaaat tgaagatggc ggaccagtga cagttactca
    20401 tccagatatc attagatatt ttatgacaat accagaagca gtaggcttag ttatacaagc
    20461 tggggctatg gctaaaggtg gagaaatatt tgtgcttgat atgggagaac ctgtaagaat
    20521 attggattta gcaaaaaact taattaaatt cagtggattt gagccagatg ttgatataaa
    20581 aattgaattt tctggtctta gaccaggaga aaagttgtat gaagaactac ttatgtcaga
    20641 agaaggtctg ttggatacag agcataaaaa gatttttatt ggtagaccaa tagatgttga
    20701 tagagaaaaa ataacaaaat acttaaaact tttaagagaa attactaaca atgaagaggt
    20761 tgaaaaaata gatggtatta tgagggaatt agtgccaact tatattaaac cagaagatgc
    20821 taatatcaaa gaaattgcaa caactagaga aaagtagaat atatttctaa tttgtaacca
    20881 gggggagcaa aaagaaacaa atatatacta aaaagaaaca aaagattaca aaaagattac
    20941 aaaaagatta caaaaaaggt tgcaatttat tttgatgtaa gatagaatgt taattaggaa
    21001 ttggaaacga aggaacaaaa tgttaaattt tcgaaaggat taggtatttg agattatgat
    21061 gaatattaaa aataaaaaac atatattgaa aaaatttata gctatggtgt taatagcagg
    21121 agttgtcaca gtagaagcag gagcaataac ggctagtgca gcagagccaa ctaattcacc
    21181 tatgagtgca acagtagacc agtgtgattt tttaaatgta agaagtggag ctagtgcaaa
    21241 tgatgcagta gtaggaaaga taaacacagg agacaaagtt gaagttttag aattacattc
    21301 aaatggatgg attaaaataa aaagtgttga caatgttaca ggatgggtaa atggagatta
    21361 tttaactatt caaggtggaa atgtagatgc aaaggtacaa aatgttttaa acttagcatt
    21421 taagcaacaa ggtaagccat atagatgggg agcaactgga ccaaatgctt ttgattgttc
    21481 tgggttcact tcttatgtct ataaaaatgg agcaggggtt aatttaccaa gagtatcaag
    21541 atcacaagca acagtaggaa agaaagtatc aagagcagaa ttaaaaccag gagatttagt
    21601 tttctttggt tcaggtggtt caataaatca tgttggattg tatgtaggag attctaaatt
    21661 catacattca ccacaaacag gagatgtagt aaaagttact agcatggctc caggaactaa
    21721 ttatgcaaga agacttataa ctgctacacg tgttttacaa taataataga tattgattta
    21781 cataggattg aatattaaca ttaaatatat aattacattt aatgttttac atagagattt
    21841 aagagtttag taatattaaa atagtgatga acgttataaa aacagcaaag aagataaatt
    21901 tctttgctgt tttttttata ataaattatt tcatattata atcaaatatc aaatataaat
    21961 tttaactata ttattcaata tttgacagat aatatgaatt ttagatatac ttaataagaa
    22021 gtatattgta gaaaaaataa atttaaaaaa ttaaggggga tgaaatgaaa aaaataagta
    22081 tattagtatt atcattgata atgacactta ctatgtgtag tgtaagttca tttgccgatt
    22141 catcaaatga taaggaaatg agagctgctt ggatatctac agtttataat ttagactggc
    22201 ctaagactaa gaataatgaa gccaagcaaa aaaaggaata cacagattta ttggacaaac
    22261 taaaaagtgt aggaataaat acagcagtgg ttcaagtaag accaaaatca gatgctttat
    22321 ataaatcaaa tataaaccca tggtctgaat atcttaccgg tacacaaggt aaagaccctg
    22381 gatatgaccc attaccattt ttaatagaag aagcacataa aagaggaatg gaatttcatg
    22441 catggtttaa tccatataga ataactatgg cagatgagag catagataaa ttgccagcaa
    22501 accatccagc aaagaaaaat ccaagctggg tagttaaaca tggaaataag tattactacg
    22561 acccaggttt accagaagtt agaaagtaca tagtggatag tatagcagaa gtagttcaaa
    22621 attacgatat agatggagtt cattttgatg attacttcta tccaggagta agtttcaatg
    22681 atactgcaac ataccaaaaa tatggaaaag gacaaaataa ggataattgg agaagagaaa
    22741 atgtaaatac tctacttaga gatgtaaaag cgtctataaa gagtataaag cctaatgttg
    22801 tatttggtgt aagcccagca ggtatatgga gaaataagag tagtgaccca acaggttcag
    22861 atacatcagg taatgaaagt tatgtaggta catatgcaga tacaagagca tggataaaac
    22921 aaggattgat tgactatgta gttccacaac tttactggcc aattggtctt aaagcagcag
    22981 attattctaa attagtggca tggtgggcta atgaagtaaa aggaacaaat gtagacttat
    23041 acataggaca aggtatatat aagcaaggtc aatctagtta tggtggacaa aatatagcaa
    23101 aagaaatagt acaacaagta acattaaata gaaagtatag tgaaataaaa ggtagtatgt
    23161 atttctcagc taaagatata gcaaatagta cttctataca aaaggattta aagagtttat
    23221 attctagtag tgaagaacca gttactccac catctaatgt taaagtggag aaattaagag
    23281 gcgacgaaag atatgacaca gcagtagcaa taagtaaaaa aggttgggct acaaattcag
    23341 atacagtagt attagtaaat gggtactcta tagttgatgg cataacatca acaccacttg
    23401 ctacatcaaa tgatgctcct atactattgg taaataagga taatatacct acatccacta
    23461 agaatgagtt aaaaagactt aatccaagta aagttatttt aattggagga aataactcta
    23521 ttggagataa agttgagagt gagataaaag atacattgtc aaatgtatca ataaatagag
    23581 ttggtggttc agatagatat agtacatctt taatgatagc aaaagaatta gtaaaaacta
    23641 atccagtaga aaaactatat ataacaagtg gtacaggtga agcagattct ctttctatag
    23701 cttcaaaagc aggagaagaa aagcaaccaa tagtactagt atcaaaagat aatgtaagtg
    23761 atgaagttta caattggata tcagatttaa aagtaaaaga tgcttatttt ataggtggta
    23821 atctaagtat atctgattca gtaataaata agctggataa ggtcattaca aatgatgtat
    23881 ctaaaaatag aatagctgga gaaaatagag aagaaacaaa tggaaaagta atacaaaaat
    23941 tctatccaaa tgctgaatat tcatcaatgt ttgtaagtaa aagtaatcaa ttggtagatg
    24001 cattaacatc aggtccttta gcagctaaat taaaatcacc agtagttatg cttggaaata
    24061 gtgtaacatc tgctcagaaa actgctttag agcctaagaa aactgctcta gtatacgaag
    24121 ctggagatgg tataaatcaa aacacattaa acactttctt aaacttagtt aagtaataaa
    24181 aaagttaatt taaaatacag agtattttat aaatgtagat taaaatgaaa attatcatca
    24241 taaattagaa aataaaaaag aactatatgc attttgtaat caagtgtaca cagcaacttg
    24301 aaaataatta tgcgatatag ttctttttta gtaattaatc ttatactttt taataaactt
    24361 tatgttgaat tttttattgt ttaaactcta tgtcattaaa tataaaatcg tgtaatatgt
    24421 ctaaagtatc tgggtcaaat ctaagaaccc atccagcttt tccatatata ccacctgtac
    24481 tatgaatttc atcatcaata ggaaattcaa attgcttaat atttaagtca cccattttta
    24541 aaacttgagc tcctaaagaa agtattgcat taggcttcat gttagtttta acatatggaa
    24601 gaactgcatc taataaacct ggatatttag taacaggtaa agtttccgtt ttcttgatta
    24661 tagcttgtat aatctgtctc tgtctgccat ctctagcaaa ggctgtatcg ttgtgtctga
    24721 tacgtgcaaa agatagtgcc tgatatccat ttagagtctg ctttccagag tttctaatgt
    24781 attgtatgct acctttatca tcacttttat tccatttata agtttcagga ataaatttat
    24841 ttagttctcg tatttctcct ttttcaacag taacctcaac gccaccaata gcatcaatta
    24901 tatacataaa tgattcgaaa ttaacacaag cataattttg aatatcaata ttaaaattct
    24961 cttcaattgt ttgtattaat aaatcagctt gtccataagc ataagcatga gtaagttttt
    25021 gtttaccatg accagggatg tctacatagc tatcacgagc taatgatgta agtttgatat
    25081 cattgtgttt gttatcaata gtaagtatca tcatagcatc tgaccttgaa gaatcttcat
    25141 ttggtctagc atctgttccc ataagtagta tgttaattat cccatcttca tttttatggt
    25201 cattgttatc caataaatca ctacttatgc tcgagtcatg aatagcagat aatttataat
    25261 aaaaatatcc atagacactt atagggaata ttactaccaa gaaagccagt agtataacaa
    25321 atttctttaa ttttgacaaa agtacccctt ctttcttctt tttcctgtat attttttaat
    25381 agcatacact taattatatt aaagtattga gatatattca atacttgttt cagttacaaa
    25441 aaaaagacaa tgatataatt tataagaaaa tttatcgacc tatatgtata gttaaaagta
    25501 tatataatat attttttaaa agtaatttat attttaaaat aaaaacacac cccataaata
    25561 caaagcaatt tgcatttaag aggtatgtat ttatcatcaa attattggtt tgttttggaa
    25621 tttctaatct tcaaccataa tatctttaaa tatgaaatca tgcattgcat ctagttcata
    25681 ttcctcaaaa ggtattacat agcctgcatt acctattttt ctaccatttt cagtagaaag
    25741 aggaaactcc atagatttca aatttaaatt tcctatactt aaaagttctt ttcctaaaga
    25801 tattatttca ttaggattca tattagtttt tacatacgga agtattgtgt taactagatt
    25861 aggatattta gtgactggta aatccttaac accatttatt aaactctgta taacacttct
    25921 ttgtcttcta tctctttcat ctgtactatc attttttctt atacgagaaa atgcaagtgt
    25981 ctgatatcca tttaattttt gctcaccaga atttcttata tatttaagag aacctttatt
    26041 gtcattttta ttccatttat aagtttcagg aataaattta tttaattctc ttatttcact
    26101 cttatcaaca gtaacagtaa ctccacctaa agcatcaact atatccataa aagagaaaaa
    26161 gtcaactacg gcgtagtttt gtatatcaag ttggaagttt tcttctatag tttctattaa
    26221 taagttagct tgtccatata cgtaggcatg tgttaatttt tgttctccac gtcctggtat
    26281 atcaacataa gtatctctat ttaatgaagt tagcttaaga cttttatttt tattatcaat
    26341 agtaagtatc atcatggcat cagaacgaga aacttcttct cctggtcttg catctgtacc
    26401 taacaataat atgtttgtga tattttcttc gtttttatag tcattactac taagcgcctc
    26461 tgtatttata ctatcatctt gtatagtttt taatttagta aaaacatatc catatatact
    26521 aacaggaaat gctataacta aaagggctag aaatataacg aactttttga gtcctgacaa
    26581 taatatcccc tactttctaa attttttaat tacacatagt taattatatt aaaaatttgt
    26641 tacataagca ataaaaaaca caataaatta gcataagttt gtcataatat aattattttt
    26701 atagtatttt atagaagaaa gtgtgtagtt attattgatt ttattggaat ataattgtat
    26761 gaagacttaa atttttataa actgatgtgt atattgaaaa aaatattttc ctgaatgtag
    26821 ttatagcatc ttatgaaagg tgtaaattgt aatttaaaat ttttagtgta taataagaat
    26881 tgaaaattca aaccgataaa aattaataaa aaataattat aaattaataa tttgggggag
    26941 gaaatgtatg ataaaattaa tagcaacaga tttagatgga actttgttag atgaaaagag
    27001 cgaaataaat ccagagtttt ataaagtatt taaaaaatta agagagagag ggataatgtt
    27061 ttctgctgct agtggcaggc agtatcaaaa tcttataaaa aaatttgaag atataaaaga
    27121 tgatatgatg tttatatcag aaaatggaac actagtagta tataaaggaa aagaaatatt
    27181 atcaaatcct ttaaataaag aattagtaaa tgaaatcata gagacaacaa gaagtataaa
    27241 agggaaaaaa attgtaatga gtgggaaaaa atacgcttat atagaaagca aagatgaagc
    27301 gtttatacaa gaggttagta cttattatgc aaagtttaaa attgtagacg atttgacgaa
    27361 agtagaaggc gatatattga agatagctgt atttgacttc aaaggtgcag aacataataa
    27421 taatatctac tttgaaaagt tttctgatag agctcaagta tgtatttctg gagtggagtg
    27481 gcttgattta actgcaaaag gtgcaaataa agggagtgcc ataaaaaaag tacaaaaaat
    27541 gttagatata aaatatgaag aaactatggt atttggtgac cagttaaatg atgtggaaat
    27601 gatgaaaagt gcctatcata gttatgccat ggaaaatgca aatgaacatt tgaaacaaat
    27661 tgctagattt agagcaaaaa gaaatactga aaatggcgtt gtagataaga taaaagaggt
    27721 cataaaaatt ggatagtcat atgtatagat gaaaaagatt attttgaaga aatcaaaaaa
    27781 atatcaatgt aattttgata aataagttta tttatgcgaa aattacattg atatttaata
    27841 aacatcaata atagatattt atttttttat gatattcagt attttgtttt ctatttgagt
    27901 tgcattttca gcagaaatta aataaacata tctacctgct ttctcaatta tagtattatt
    27961 tgctatttca gaattgattt tttctccatg tccttctcca aaaggtgctt gtatcatatc
    28021 ttttttataa ttttctagag ttttatatat tttatctacc tcgtcagttt ttataacgat
    28081 aatatcttct agagataatt tttcagaaga attaattaca aaaccttgaa ttatgtcttc
    28141 ctctacatca ttaaaatagt taaagtcctt tgcatcaaag atttttgatt cttttataag
    28201 taaatcagaa gattttaatg ctttttttat ttttgatgta gaaacgtttt tgtaatcacc
    28261 acttgaagaa caactaacca ttcctaatga taagattatc gaaaaaaaca atattaaaat
    28321 ttttttcatg ataaacctcc aagtttttta aactctgtct taaattagtt tactacataa
    28381 ttatataaaa ataaatgtat ttaatgttac agttttgatt aataatgtaa tattttgata
    28441 ataataccta ttgagaggtg attagaaatg agaataccaa aacatgtagg aattataccg
    28501 gatggaaata gaaggtgggc aagtcaaaat ggtatgacaa aagataaagg atatgaaagt
    28561 ggaatagacc ctggattaga gtcttataga ttatgtagag atttaggctg tgaagaggtg
    28621 actttttatg gatttacaac tgataataca agacgtccat cagtgcagac aaaagctttt
    28681 acaaaagcat gtattgatgc tgcaaatata ttaatagatg aaggtgctgc tttattagtt
    28741 gtaggaaata caaaatctcc aatgtttcca gaagaattat taccatatac taatagaatt
    28801 aaagccaaag aaggtcaaat aaaagcaaat cttctggtaa attatgggtg gtattgggac
    28861 ttaaataata tatcaggagt agaaaatgta aataaaaata atatacagga ttacatacag
    28921 tcatttgatg tatccagact agatttaata atcagatggg gtggaagaag gagattaagt
    28981 ggtttccttc ctgtacaatc aatttatgca gatttttatg ttatagataa ttattggcca
    29041 gattttaaga gtgacgatat aaaggacgca tttagttggt atgatacaca agatgtcact
    29101 ttaggtggat aattaaaaat attctgattt aaggttgtag agtgttttaa attattgaaa
    29161 aaaaaacttc ttgatggtaa aataacacta ataatatgta aaagtgtaaa aatttatacc
    29221 aagaagggtg aagaaatgtc aaaaggcaat aataacaata attcaagaaa caaatctaaa
    29281 aagaccagtc atctgaatag aaaaaagcga aaattaaata agaaaaagtt agcagtgttg
    29341 atttgcttta cagttttatt tttatttatt gcatttaagg ctactcaagg tgtagtagct
    29401 ttggtaaaaa gtatggataa aagtaataaa actagtcaac aacaaaaagt taactcagaa
    29461 caatttgacc ttggtaatga agaggaaaat aaaaagaaaa aatatactgt atttatagac
    29521 ccaggtcatg gaggcaatga taaaggaact gaaagcaaga ccagtaatag atatgaaaaa
    29581 gacctaaatc tacaaattgc aaaaaaatta gcaaataaat tatctaaaca aaaggatatt
    29641 caagtagtag tatccagaac tgatgataca tatatatcat taaaggatag agctatctta
    29701 gcaaataata gttctgctga tgtgcttgtt tctattcatt taaatgcaga gaaaaatggt
    29761 aatacagcaa caggtataga aacttggtat aggaataaag ctacagatgg ttcaaaagaa
    29821 cttgcacaga cagtacaaag tactatagta tcctatgtta aagttagaga taggggaata
    29881 gttgaaaata actttgaagt acttagagaa tcaaatatgc ctgctatatt aatagagtgt
    29941 ggttttttaa caacaccttc agaggaacaa aaaataataa atgaaaaata tcaagaccaa
    30001 cttgcggaag gaattgttca aggtgtactt tcatacctag attctaaagg gaataagtaa
    30061 gttaacttta ctttaatgtg aggataaatt taaaagttta actgtgtaaa ttgtattaac
    30121 tttatttcgt aatgttggaa acattgtttg aatttagaaa ctatgaaatt agaaattatt
    30181 ttatctatat ctaatttcat agtttttttt gcaaaaaatt tatagttata aaatttaaaa
    30241 aaataaa
//
LOCUS       DAEPOZ010000019        30041 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.019, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000019 DAEPOZ010000000
VERSION     DAEPOZ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30041
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.019"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..952
                     /locus_tag="KR440_000716"
     CDS             <1..952
                     /locus_tag="KR440_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBH0697779.1"
                     /translation="LSESMSYFKNTLGIDRVKNVEKIIEPIFDYKNRVSVVGFSDICE
                     YRNSEFPNEMSKIISNISKITEGHTLALFNSKDRQEKTYEILKKYLHSFNLEIYADKK
                     GIRHLNDLNRKCVVLGSKGCFEGVDIPGDGLVCVTLDKLPNLNPKDPLYFTIMKKYGI
                     DYYTINYPQMTIKVKQAMGRILRSKYDYGCFVIFDVGTNISVLKRLEKDLHDCKISKV
                     NSNEFYTYIRRHLNKSRSLILKSVIFDTIKALNVDAKMDNNDVDKDIIKKDINENIRQ
                     RAVKGEVYHIDIIKKDMKVKYFDRNYLINLDIFMREEDKN"
     gene            970..1569
                     /locus_tag="KR440_000717"
     CDS             970..1569
                     /locus_tag="KR440_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HBH0697780.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(1826..3955)
                     /locus_tag="KR440_000718"
     CDS             complement(1826..3955)
                     /locus_tag="KR440_000718"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBH0697781.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            4221..4469
                     /locus_tag="KR440_000719"
     CDS             4221..4469
                     /locus_tag="KR440_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697782.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKAIHKTRTKTVSTNKKQKSHSRDIKRKIS"
     gene            4635..6026
                     /locus_tag="KR440_000720"
     CDS             4635..6026
                     /locus_tag="KR440_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBH0697783.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKEDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            6141..7610
                     /locus_tag="KR440_000721"
     CDS             6141..7610
                     /locus_tag="KR440_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBH0697784.1"
                     /translation="MSNVNKKLVIAIGAVALVAIIFIGIMCMQFKGEKIAKNTYVNGV
                     DIGKLTKSQAKQELAKKYKLENVEFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SSFFGNLSKTISTNFGKKSNLVVVINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLTNIDTNLGTY
                     STIFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            7675..8268
                     /locus_tag="KR440_000722"
     CDS             7675..8268
                     /locus_tag="KR440_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HBH0697785.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            8450..9796
                     /locus_tag="KR440_000723"
     CDS             8450..9796
                     /locus_tag="KR440_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBH0697786.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDTSKKEEIRKVKRKKSILPYIIVGCVLIGMGIYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPEREITVADEEDLYYQAL
                     NLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            9978..10349
                     /locus_tag="KR440_000724"
     CDS             9978..10349
                     /locus_tag="KR440_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBH0697787.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            10342..11031
                     /locus_tag="KR440_000725"
     CDS             10342..11031
                     /locus_tag="KR440_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBH0697788.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGIESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            11551..12417
                     /locus_tag="KR440_000726"
     CDS             11551..12417
                     /locus_tag="KR440_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBH0697789.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            12659..13468
                     /locus_tag="KR440_000727"
     CDS             12659..13468
                     /locus_tag="KR440_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697790.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKKGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGQGSYTFFVDEGT
                     AMEYIGEFKEDKKNGKGNFVFSNGNEYIGHFEEDMMNGIGNMLYNSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            13611..14675
                     /locus_tag="KR440_000728"
     CDS             13611..14675
                     /locus_tag="KR440_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBH0697791.1"
                     /translation="MKRLIALVLVILIVLFYSPELIEEGYDLKEKFFQSEIWNEFLDK
                     ININNNSKKDKKEQNQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     KLYPDDREELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HINKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            14703..15422
                     /locus_tag="KR440_000729"
     CDS             14703..15422
                     /locus_tag="KR440_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBH0697792.1"
                     /translation="MTSWKKKTVYKCLIAVVLFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKDLI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVKNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            15446..16030
                     /locus_tag="KR440_000730"
     CDS             15446..16030
                     /locus_tag="KR440_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HBH0697793.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGEAVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGYVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIIDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLENFITEARERVHM"
     gene            16032..17381
                     /gene="pabB"
                     /locus_tag="KR440_000731"
     CDS             16032..17381
                     /gene="pabB"
                     /locus_tag="KR440_000731"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HBH0697794.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDTHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            17371..18114
                     /locus_tag="KR440_000732"
     CDS             17371..18114
                     /locus_tag="KR440_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HBH0697795.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDINYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FIKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            18148..18849
                     /locus_tag="KR440_000733"
     CDS             18148..18849
                     /locus_tag="KR440_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697796.1"
                     /translation="MIGCIYEVYNEEMTKIIENQNTLAVYLVGSSKDVDFTLYDVEIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISNIIKDISRDIYLEGPSKMSLEEKSFIKQDIGAKISNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELASKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            18875..19447
                     /gene="folE"
                     /locus_tag="KR440_000734"
     CDS             18875..19447
                     /gene="folE"
                     /locus_tag="KR440_000734"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBH0697797.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            19513..20304
                     /gene="folP"
                     /locus_tag="KR440_000735"
     CDS             19513..20304
                     /gene="folP"
                     /locus_tag="KR440_000735"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBH0697798.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELEIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            20315..20680
                     /gene="folB"
                     /locus_tag="KR440_000736"
     CDS             20315..20680
                     /gene="folB"
                     /locus_tag="KR440_000736"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HBH0697799.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            20673..21179
                     /gene="folK"
                     /locus_tag="KR440_000737"
     CDS             20673..21179
                     /gene="folK"
                     /locus_tag="KR440_000737"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBH0697800.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDILFFN
                     DVASTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTVKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            21160..21945
                     /gene="aroF"
                     /locus_tag="KR440_000738"
     CDS             21160..21945
                     /gene="aroF"
                     /locus_tag="KR440_000738"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBH0697801.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLKILKAVKDEVGMAVITELMDVRD
                     IDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            22200..23990
                     /locus_tag="KR440_000739"
     CDS             22200..23990
                     /locus_tag="KR440_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBH0697802.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLDCGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            24008..25174
                     /gene="rpoD"
                     /locus_tag="KR440_000740"
     CDS             24008..25174
                     /gene="rpoD"
                     /locus_tag="KR440_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBH0697803.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            25339..26028
                     /locus_tag="KR440_000741"
     CDS             25339..26028
                     /locus_tag="KR440_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBH0697804.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            26042..27139
                     /locus_tag="KR440_000742"
     CDS             26042..27139
                     /locus_tag="KR440_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBH0697805.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSIEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            27201..27773
                     /locus_tag="KR440_000743"
     CDS             27201..27773
                     /locus_tag="KR440_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBH0697806.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEVLEEMNIEVDIFRVSDEVAKT
                     LPQYYLINYKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGAPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(27833..28300)
                     /locus_tag="KR440_000744"
     CDS             complement(27833..28300)
                     /locus_tag="KR440_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBH0697807.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRI"
     gene            complement(28686..29162)
                     /locus_tag="KR440_000745"
     CDS             complement(28686..29162)
                     /locus_tag="KR440_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBH0697808.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLRVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(29282..>30041)
                     /locus_tag="KR440_000746"
     CDS             complement(29282..>30041)
                     /locus_tag="KR440_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HBH0697809.1"
                     /translation="EIYSKLGHIDVDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTL
                     GMSIFIAFNYNKLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIG
                     SIKSRIKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLSTAMN
                     ITTLLVIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIG
                     WTINFGNPLGKILYIAIALLLIFSLFSLIKSLLL"
BASE COUNT        12211 a         3087 c         5269 g         9474 t
ORIGIN      
        1 tttatctgaa agtatgtctt attttaaaaa tacattagga atagacagag ttaagaatgt
       61 ggaaaagatt atagaaccaa tttttgatta taaaaataga gtctctgtag ttggattttc
      121 tgatatttgt gagtacagaa attctgaatt tccaaatgag atgagtaaaa ttatatctaa
      181 tatatcaaag attacagaag gtcatacatt agctcttttt aatagtaaag atagacaaga
      241 aaaaacttat gaaatattga aaaaatactt acatagtttt aacttagaaa tttatgctga
      301 taaaaagggg attagacacc ttaacgattt aaatagaaaa tgtgtagtac ttggctcaaa
      361 aggatgtttt gaaggcgtag acattccagg ggatggtttg gtttgtgtaa ctttagacaa
      421 gcttccgaat ctaaatccaa aagacccttt gtattttact ataatgaaga agtatggtat
      481 agactactat actataaatt atcctcaaat gaccataaag gtaaagcaag ctatggggag
      541 aattttaaga agcaaatatg actatggatg ttttgtgatt tttgatgtag gaactaatat
      601 aagtgtttta aagagattag agaaagattt acacgattgt aaaatttcaa aggttaatag
      661 caatgagttt tatacttata taaggcgaca tttaaataaa tctagaagtt taatactaaa
      721 atctgttata tttgatacta taaaagcgtt gaatgttgat gctaaaatgg ataacaatga
      781 tgttgataaa gatattataa aaaaagatat aaatgaaaat ataagacaaa gagctgtaaa
      841 aggagaagtg taccatattg atattataaa aaaagatatg aaagtaaaat attttgatag
      901 aaactatctt ataaatttag atatatttat gagagaagaa gataaaaatt aagagtggga
      961 gtgagatatt tggcaaaaca aaagttttat gctgtgaaaa aaggtaaaaa tataggtgta
     1021 tataatacat gggatgagtg caaaaaacaa gtaaatggat tctcaggagc tgaatataag
     1081 agtttttcta cttttcaaga agctaaagaa tatattgatg ggtcagaaaa attgagtttt
     1141 caagaagata aggagtttat agaagcatat gtagatggaa gttatgaaca ttcagtaaaa
     1201 atgtatggtt cgggtgttgt gattttaaaa aataatgaag ttataaaaac ttatagtgaa
     1261 aaaggaaaag agaaaacttt agtaagtatg agaaatgtag ctggagagat agaggcttct
     1321 aaaattgcaa tgcagtactg tattgacaat aatgttcaaa acttgatttt atactttgac
     1381 tatgaaggga ttgaaaagtg gtgtactgga gtttggaaaa caaataaaga aggaactata
     1441 gcatataaaa atttctatga cagtattaag aataagctta atgtaaaatt tacaaaggtt
     1501 aaagctcatt ctggaaataa gtataatgag gaagctgata aattagctaa aaaagctatt
     1561 ggtgtataat aaaaaaatat ttatacataa ttacatggta taagagtata tctaaattcc
     1621 tactttataa ttaaacaact tgtaattaaa caacttgtaa ttaaacaact tataattaaa
     1681 taaattttga ttaaaaaagc agattgtatt atgaaaaaat aagtatctaa tttataaaca
     1741 attaataata gtattaatta gatacaactt atttaatata ataaaatctg ctttttatat
     1801 aactataaaa tcttcctaca ttctattaga attgcccata aataccttca agttcttctt
     1861 ttaatacttc agggtcattt ataccaatag acatggttct agccagtata tttgcttggt
     1921 cataagttga tgtccatgag aatacaccac ctagacaatt tctaagaaca tattgagttt
     1981 ttaagaatat tgataaagca tcatcatatg acattgcaaa atctccatcc ttaactaaat
     2041 acggaacttg agcagtatta tcaaatctat attcatatcc atttttattt atataatccc
     2101 ttctaagctc atcataagtt cttgttatag tggcacctaa tcttccataa aatggtatac
     2161 cgagaaggat tttttcagaa ggcattccag cattctcaag atttctcacc attgcatcaa
     2221 cactatatcc tggcaaagat aagtctgaat caaaaagatt tgcttgatgt tttctaccat
     2281 ttgggcctgt ttcacctgct gtaaaatcat aactcataag attaaaataa tctattatag
     2341 gagctatttt atctatttca gcacttgaat ttatatatcc tctatctcct gttccagcta
     2401 cactaagcca tttatcatct cctataacat ctcttatggc agttagtaag agtgtaaagt
     2461 tttctctatc ttgaggtctt gatgttattc cagatgcact acttccagga tattcccagt
     2521 ctatatctat tccatctaaa gcatattcat ttatcatctg attgacctgt cttgcaaaat
     2581 tatatctaga tgtaggtgtt aatgctgcat cagagaatcc ttcagctccc caacctccaa
     2641 tagctacaat tacttttaag ctaggttttt cacctttaag agcaagtaat tgatttaaaa
     2701 atctaggata aggaataaac aaatttccag actggtctat ctcagcaaaa gcaaatataa
     2761 ctgcatctaa tagttgagca tctacattag ttggatttcc aagaacatat tctgctacaa
     2821 ttggatttat atctggacag tttgcatttt caactattgg attgtatggg tctgttattt
     2881 gtggtctaac taattttttt gaatctttta aagttttcat agctattttt ttagtatttt
     2941 tttcgctagt ataatcgtag tttttataat tatgtttata gaaatcttca taactactat
     3001 cttctttgtt atagctagaa tttattttgt cgcaagattc atctttatca tagttaaaat
     3061 tcatttcatc acaagactta tctttaaact tatcttgttt ggagttgtga actttgttag
     3121 tatttttagt atgttttttc tcatggttgc aatcatcttt aatatcagtc ttgtgttctt
     3181 gtttacagtc aagagctttg ttcaggtagt catgatgttc atgttcacag ttacattcat
     3241 tttcaatgtt tttagtatgt tcttttttcc agctacaagt attatcaatt ttcttgctca
     3301 cttcttcatc attataatta tctggtacaa aacataagta ccagtccata catgaatatt
     3361 tcttattaca actgttaact ctatttttta attccttata gtttttagga taaggtaaaa
     3421 tcactggcat tccaggaaac cagtttgcag gagtaactat attatctcta tcactagtct
     3481 gaagtgcatc tactattcta agtatttctg gaatatttct tcctgtagtt agtggataat
     3541 aaagaatcgt tcttagaatt tgattattat ctataataaa tacagagcga acagttgatg
     3601 tatcactcat tggttttgat atcatgccgt ataacttggc aattctcata tctctatctt
     3661 ctataatagg aaatggaatt tctacacctg taagtaaaga aatattatac atccaagcta
     3721 aatgtgaact gttactatca acacttagac caattagttc tgtatttctt tttttaaatt
     3781 cgtcataata tttagcaaaa cataaaaatt ctgtagtaca aactggtgta aaatcaccag
     3841 gatgtgaaaa taaaacaatc caattaccct tatagtcaga gagtctaata ggaccatttg
     3901 ttgtattggc tttaaaatca ggagcctttg accctaaact tggcaaattt ggcatgtaaa
     3961 tcacccctca ataaattatc atttatttta ttctattaat taatacatat tagtgttact
     4021 attttattaa aagttaagta atccttatgt caaaaaaaga tatttattaa aaagatttaa
     4081 tattttccta atataaatga caatagtaat tgctattttt ttactaattt ggtatagtaa
     4141 tggataaggg gtagtgtcaa ttttagtgat tatacgaggt gattggattg aaaaaaattc
     4201 taagaatttt agttatctcc atgatgatat gttttgcaat gacttttgca ttagtgttaa
     4261 taaattcagt tatgggagta catataggat tggattatta ctttttgatg ggaagtgtgc
     4321 ttacagtttg tttaacaatt ggaattgttt tatgcaatgc tagaaaaata tttgatagca
     4381 aagctataca caagacaaga acgaaaactg tcagtactaa taaaaaacaa aagagtcatt
     4441 ctagggatat aaaaagaaag atatcataat ttatgtaaag gaaatattta tgtataaaat
     4501 aatgattgat aggtaaaata aaaacacgat ttaacttgtg tttttatttt ttttcaagaa
     4561 ttatgatata ataaccaata agaaatttag tttaccataa aatataaaga ttattaaatg
     4621 gagagggaac tataatgaat tttagagatt tatttgagaa ggctgtagat tttattgatg
     4681 aaaaaaggaa atctatagtt gcgatttcat tatcaacatt aggtgtaatt gctttgataa
     4741 tagtattttt cttaagtagt aacgagttta gtgtaggaaa tgaagctaat gagttgttaa
     4801 agattataga gaaaagacag tattctatag ctgttgatta ctacacaagt atagaaaaaa
     4861 agttttctga ttcaaaaatg gaaaggttta ataagtctgt ttctaaaaaa ataaataaat
     4921 tgctattaaa tagtggtgat aaatatttag atggagacat aagtcaagaa agttttattg
     4981 gattaataaa tactgtgaaa gaattaaaca aaataagtat agatgtggat gacttattag
     5041 ctcaagctga tagagttaga gaaatgtata aagaagaaaa tactacttat gatattgcca
     5101 ttaactacat aagtactgtt tcaatattaa atggtatggg tagtaaccta gatgtataca
     5161 aacagaatat agaaacaatc aaagagtcta gagatgttta tgactcagca gtaaaagacc
     5221 aaaaagcata taagtattat gaagctattg aaaagtataa taaagtatta aaagaagatg
     5281 aaaagtatta cagtatggct caaaatggga aagaacaatg tattgaagaa atgtatgatt
     5341 actatataag tagagctgaa gaggcaaata cttctggaga ttatgaaaga gcattacaat
     5401 atattgagta tttaaaagaa gattattcag atgatgaaaa ggtacagagt ttagaaaaaa
     5461 aatataaaaa gaatttatca ttgtatacct taacatctga tgacattata aatgtaatag
     5521 ctaaaaaaag tggcaaagac aaggcaaatc tatctataaa ttcacttcca caaatgataa
     5581 aaaataataa atactattat gctgaagtat atgagtatga taaattaata aatgaagttt
     5641 tggtaagtgc aaaaactaaa gatttatatt cgtataaaga tggtaaaaaa gattacaaag
     5701 tagattatgg tgatggatac tttagaatat tagaagatgg aagttatcaa tttggtatta
     5761 caaaagataa agctaagttt gtgttaacta atactcttga tgaaaaagaa aataagtata
     5821 aaaagattga gatattagat atagagaaag cagatagata tgtaaaaagt aaaaagagtt
     5881 tagaagaatt gtttggaaaa tataaaaata tttattatta tgctgtagta aataaaggct
     5941 tatttagagg caaagaagtt tatgctatta atatttacaa tgaaaaaata tatgccatat
     6001 cagaaaatgg actaaatgaa tattaattaa taatattaaa ttaatatata attaaaaagt
     6061 ttttggggtt ttaaggcgca ttatgtagta atataataat agggatatat aaataatttt
     6121 attgtaagga gaagtatggg atgagtaatg tgaacaaaaa gctagttata gctattggag
     6181 ctgtagcatt agtagcaata atttttatag gcataatgtg catgcaattt aaaggggaga
     6241 aaatagcaaa aaacacatat gttaatggag tagatattgg aaaattaact aaatcgcaag
     6301 caaaacagga attagctaaa aaatacaaat tagagaatgt tgagtttaat tacaatgata
     6361 aaagttggaa agtaaagagc aaagatttaa atttatctac tgatctggat aaaacagtag
     6421 aaaacgcata taatttaaat agaaagagta gtttctttgg caatctatca aaaactattt
     6481 caacaaattt tggcaaaaaa agcaacttag tagttgtgat aaattatgat aaaaataaat
     6541 taaaagcaga aatggaaaaa atagctaaag aaatagatgt agatgtaaaa gatgctacac
     6601 ttgatataag tggtgaaaaa gtcaaggtta taccagattc tgatggtctt aaaatggata
     6661 tttctaagag tatggagaat tttgataatc aaactaaaaa aggtaactat aaaaatgagt
     6721 tagtagtaaa agctactcca gcaaaagtaa aaaaagagca acttactaat atagatacaa
     6781 atttaggtac gtattcaaca atatttaaaa cttctcaaat aaatagaagt ataaacataa
     6841 aattagcaac agataatata agtaatgtat tacttatgcc aggagaaact ttctcattta
     6901 ataaacatac agggaaaaga agtaaggaaa atggatataa aagtgcacct gtaataatgg
     6961 aaggtgagat ggaagaagat tatggtggag gagtttgtca agtttcatct acgttataca
     7021 attctgtgtt atacgcaggt ttagaaattg ttaatgtaaa aaatcatact ataccatcaa
     7081 gttatgttcc aaaaggaaga gatgcaactg ttgcagatag tggaatagat ttcttattta
     7141 aaaataatct aaagcatcca gtttacataa aaaattacgt ttcaggaaat caaattgttt
     7201 gcaatatata tggaagtgca gaagataaac aaaatataac gatatcaaca aaattagatg
     7261 gcgtttcaca aactacaatg aaaagagtta atgaccctac aatgcctaaa ggtaaagaaa
     7321 aagtagacaa aagtggaaga aatgcatatt ctgtatcaac atatagaaca tttaatgatg
     7381 caaatggtaa aaaaattaaa actgaaaaaa tagctaattc atattatcct aagaaggaag
     7441 gtattatatt agttgggact atggaaccaa aaccagagga aaaaccaaat acagacgaaa
     7501 ataagaataa tcaaaataca aacaatcaaa atccaaataa tcaacaaaaa ccagaaacac
     7561 caccaactga taataagcct aatgagacac aaccacaacc gcaagcttaa agttctcaag
     7621 aataggattt atgtagagta ataaaaaata taaatctaaa taggagggat tactttgaat
     7681 ttaaataata taacgaatta cgttcaaagt atttgcgaga atatttctaa tgtattggat
     7741 gtagatgtta ctttagttac aaaagattta actaggatag caggtacagg aatattcaga
     7801 ggtaaaatag gagagacaat atcagagaag tcagtgtact ctcttgtttt aaaagatgga
     7861 aaatcctatg ttataaataa ggaaatagaa gagaactgta ataaatgtgc aaatagagaa
     7921 aattgtaaag aattagctga tatatgtact cctgttaaaa tgggagaaca tatacttgga
     7981 atcttgggta tagctgcgtt taatgaagag caaaaagaaa agatactttc aaaagataag
     8041 gagttaactg cttttgtaag gagtatgtct gatttaatat cttttaaact agatgaactt
     8101 tataaacaag aagttaagac tatagatgac cttgagaggg aagctataga aaatgccata
     8161 aagaaatatg gaagtaatac agaaggtatg aaaaaagtag ctgaggcatt aaatataggt
     8221 atagctacac tctatagaaa agtaaaaaaa ttcaacatta aaagttaata gtattgaaaa
     8281 agaggtaacc tttttatgaa aagaagagga ttaccttttt tattatgaga atggtggaaa
     8341 aatatgtata gttttgactt gacatattaa atatgttatc ttaaatatat attttagagt
     8401 ggaggtgagt attattttag ttaaagatat aaaacaagac atagaaagca tgcctagaaa
     8461 aattttagat atatctttga tagaagataa taaaagtgat ttgacttttt ttaatataga
     8521 agaagaagac ggtgatgaaa cattaaaagc attaaaacag agttatgtga ctacaaatta
     8581 taaagaagaa actctattaa acttgcaagg agctttaaat tcaaagtcag acacaacgtc
     8641 taaggagtta aaattagaat catataaaag atataacaat gcattggatt tagcatataa
     8701 aaattatata actagtgcca ttaatatggt taataaagct ctggagataa atcctaaaga
     8761 tattgatata ctaaatctta gaggtctttt aaagttatta aaatgtgatt ttgctaaatc
     8821 atttgagagt ttttatactg ctttatgtta tgagaataat catatatcta gaaaatatgt
     8881 aaatctaatt tcatctgaag attttaaaac ttttttagga agatacaatc attctataag
     8941 atttataaat gaagaattaa attatgagtc aatacatatt ttagagaaca taattgaaga
     9001 agagcctgag ctaatagagc catatgtaat cttatctttg ttatatgata aactaggtaa
     9061 tgtaaaaaag agagaaggat atttagacaa acttaaagag ctagataaag ataatcctgt
     9121 atttgaaaaa aatgaagaag aaaaagaaga tacaagtaaa aaggaagaaa taagaaaagt
     9181 aaaaagaaag aaaagtattt taccatatat tatagttggc tgtgttttaa taggtatggg
     9241 gatatatttg attcaaagca aaaagagaat agaaaacttg aataaccaaa taagtagtaa
     9301 agaggaaaag ttaagtgaaa ctgataaaaa gttaggagaa acgagtaaaa aattagaaaa
     9361 aacgaataag gaattaaatg aggttaaaaa taaagaacca gaaagagaga taactgtagc
     9421 tgatgaggaa gatttatatt atcaagctct aaatttaaag aaaaacaaag aatatgaaaa
     9481 agccatagat aattttaaaa gtgtagtttc ttctggtaaa actaaaaaat atatttctga
     9541 gtcaatatat caattagcaa taacaaataa attattagga aataaggatg aagctataaa
     9601 atattacaaa aaatatataa atacttatac taaaaatgac cagtattatg atgattcata
     9661 ttacgagttg ggtatgcttt attatgataa tggcgattta aaaaatgcac aacagacttt
     9721 ttatagtttg agaagtgaag ttccagacag tatgtataac aattcaaaaa taaaagaaat
     9781 cttaagtgaa aaataggttt atatagttaa gttttttgaa acaaccaagt atgtatttta
     9841 ttaaattaaa ataaaaataa atttaaaaag agattgtgtc aaattataac tttctatttt
     9901 aaagaaagat atttgcttgt tgataaaact acaaatatat gatttgaagc aatctcttta
     9961 atttttggag gtggaaaatg aaagttttca atcaattagt aataatttta tctatctggg
    10021 taataggaga gtatataagt tcctttatac aaggaattat acttattcct gggagtatag
    10081 ttggtatgct attattattt ctactactac aatttaaagt tttagacttg agtagtatag
    10141 aaaatgtaag tggatttttc ctcgataata tggctatatt ttttattcca gcaggtgtat
    10201 cacttataaa gtcattagac ctaataaggg aaaatgtatt agtgctttta ttagtcatat
    10261 gtttaagtac actaatagta atgtatacta ctggaattat tgtggagaaa atgataaaaa
    10321 agaaggaaga aggacaaaaa aatgtataat gtattacaga caccagtatt tggtataata
    10381 gtaacaatag tttttttcaa tttaggaaga tacattcaac gaaaaacaag taatcctata
    10441 tgtaaccctt tattaattgc aatcgtagga ataatattgt ttttaagcat atctaaaatc
    10501 ccttatgaga attacaagat aggagctgat agtctaaatt tctttttagg tcctgtcaca
    10561 gtagtacttg cagttccact ttataaacaa tttgaattat ttaaaaagca tatgtttgag
    10621 attattgtag gaataggttg tggtatagtg atttcttttg tatctgtatt gataattggc
    10681 aaaatagcta attcagatgt aagtataata aattcactaa ttccaaaatc tataacaaca
    10741 ccaatgggaa tatctttagc aaattcttta aatggtatag aatcaataac agttgtagcc
    10801 ataattttta caggtatttt tggtggtatg gttgcttcta cagtattcaa actggggaat
    10861 ataaatcatc cagttgctaa aggaattgct cttggaacat ctgcacatgc tttaggaact
    10921 acaaaagctt ttgaacttgg agaaatagaa ggagctatga gtggtgtttc tattggagtt
    10981 tctggtacaa ttacagtaat actaattcca attataatga attttatata aaaaataaaa
    11041 ttaagacgag taccttgaat acttaaaaca aaataaaata aaaaaaattt aaaataaaaa
    11101 ctgaacgaaa aaaatgcaaa taatgaaata aaaaaatcat gtactgaaat tataaaaaga
    11161 aaaatttcat ttaggaatta attataaaga gataattact aaacttgaat ttgctaaaat
    11221 aaaataaatt actgcgaaaa tagatattta gaaaaaaata ctataaattt actatattgc
    11281 aagttaaata ataagcagta tcatattatg ctagaaaaag tttcatttaa ggcataaatg
    11341 agatgatttg aacgttttta aagattagaa tataaaagat tgcactagac atatatgaaa
    11401 aagataatat aatagatgat gtaatagatg acagttcaag aaattataaa aaaaatttat
    11461 ttgaaatatt atctattaca tcaggaataa tatatgtaag agtaagcttt taaaattcta
    11521 aagatttaga aagattgaag ggagaaagaa atgagtgaag ttaagaatac cctacaaaaa
    11581 catccagttt taagatattc aacaatgcta tttgggatgt tgatgacatc tataggaata
    11641 aatgggttct taagaccagc acatctgctt agtggaggtg ctacaggtat agcaacatct
    11701 ataaactatt tgacaaatat aaatgtaggt ttattaacat ttttaatcaa tattccaata
    11761 tttatcttag ggttcatata ccttgaaaaa gagttttgta tttcaagttt ggtaaatatg
    11821 attgtatttt cattattatt aggagcaact caggaaataa gtaatattat acctatacat
    11881 gatatattgc ttcaaagtgt atatggtggt atattatctg gtttaggagt cggagttgta
    11941 tttagaacta ggtcttctca aggtggtaca gatataatag ctgcaatact aaaaattaag
    12001 aagaatatag aaatgaaaga cactgcttta gcaataaatg gcttgatagt acttacagga
    12061 agttttttat ttgggttgga tttagcatta tacactctta taggtctatt tctaaatgcg
    12121 tattcaatga gttttattaa agatgcaatg aattaccaaa aatctgttat ggtaatgtca
    12181 aatgaagtcg atttaatagc agaagatata atgaagagtt tagttagagg tgtaactttt
    12241 ttagatgcag aaggtgcata cactcatcaa aaaaagaaga ttatatatac aatagtttca
    12301 tcaaatgaga taccaaagat aaaagatata gctttgaagt atgataaaaa ggcctttata
    12361 tctgtaaatg atgttacaga agtaaaagga agagggttta aagcaaaaga tttatagaaa
    12421 attttacaag ctgggagttt ataatgtaaa tttgtatatt atatctctca gcttatttaa
    12481 atttagtata tgaaaataaa ggttttatca atgatttgtg tatcacttta aaagatatca
    12541 ttttaaaatt aaaattgaaa aaattactca atatgtagaa aacataacca ctattaggat
    12601 ataataaatt taatagattt agtaaaatat ctaggaataa cgttggagga atagcattat
    12661 gaatttatct aaagaatatg ttaatttgta tgataattat ttgaaatata taaatgaagt
    12721 aaacaaagag attagaagta taaagaacat gaagaaacgt gttttaaaaa agggcgattt
    12781 tgctccagaa aatgaagtat tagaattgga aggaaaaaca atacgcgata tagatgatgt
    12841 cgatacaaaa aaaccaaaaa ataaaatata taaattttca aatggggatg tgtatgtagg
    12901 aaaattttta gaaggaaaga tggaagggca aggatcatat actttttttg ttgatgaagg
    12961 aacggctatg gagtacatag gagagtttaa agaagacaaa aaaaacggaa aaggaaactt
    13021 tgtattttca aatggaaatg aatatatagg ccattttgag gaagatatga tgaacggtat
    13081 tggaaatatg ttatataata gcaaagatga atatattgga acttggaaaa atggaaaaaa
    13141 agatggaaaa ggtatttata agtggagtga tggatgtata tatgcaggtg aatttaaagg
    13201 tggaaagatg gaagggtatg gtatttgcta taatagtaaa ggggaagtac tttatgaagg
    13261 agaatggaag aataatttga ttcatggtaa aggaacttat atatgggaaa aaggtaaaaa
    13321 gtatataggt gaatttatgc atggtaaaaa acatggacaa ggaacttttt atctaaataa
    13381 tgaattggtt tatgaaggga cttggaaatt tgataagcca tccatcttcg acagaacatt
    13441 agatgaaata ttttcattaa gactatagct ttatctatta atagataaag cttttttcta
    13501 atacttactg taaataagta tgaatgatat tgattagtat acactatcaa tattaactca
    13561 ataaatgtga tattaaaaat ttttgattga ttttaagaag gagggatata ttgaagagat
    13621 taattgcttt agtgttagta atacttattg ttttatttta tagccctgaa ttgatagaag
    13681 aaggatatga tttaaaagaa aaattttttc aaagtgaaat atggaatgaa tttttagaca
    13741 aaataaatat aaataataat tctaagaaag ataaaaaaga acaaaatcaa aatttacaat
    13801 caaataatac tgaaggggag aacttaggag agtcagatat aaagaatttt gaaaaaataa
    13861 gcttaggaga taatttaagc tatgttttat caagtattgg taaacctggt agaattgata
    13921 ttagtgaata tggttttgat tggtatgtat ataatcaata tggaaaagaa tttgcaatgg
    13981 tcggacttga aaatgatgaa gtagtagcac tttatagtaa ttctattaat tcatgtgaaa
    14041 atcaagatat aaaactaaat caagatagac aaacagtgag aactaagatt acaccattaa
    14101 aatataagag aaaaggaaat accagatata taataaactc agaaaatcaa tatgatataa
    14161 taagtaaaga aggtaagtat attacaattt tctacgatat tcatgaagaa aatagagttt
    14221 gctcatattt aataattgat aaaagtactg aagatgagtt taaaaaatta tatccagatg
    14281 atagagaaga acttaaaaaa tgttttgaac ttgaagttat agaccttgta aatagtgtta
    14341 gaaatcaaag aggattaaac tcattgagat acagtgaaca ggcaactttg agttcaagaa
    14401 aacatagtga agatatgaga gataataatt tttttgacca tataaataag aagaatgaaa
    14461 ctccatttga cagaatgaaa agagaaggta tagtatatac ttcagctgga gaaaacatag
    14521 cagcaggaca gattaatgca atatatgcgc atgaagcttg gatgaactct gaaggacata
    14581 gaaaaaatat tttggggaac tataataata ttggagttgg agtaatattt ggaggaagct
    14641 ataaaacata ttacactcaa aatttttata aataactaaa atatttgaaa ggaagttaaa
    14701 aaatgacatc atggaaaaaa aagacagtat ataagtgctt aatagcagta gtcttgtttt
    14761 gtggaatagt gttaatatct aattttagta aagtttctgc tttgatgatg gatactaatg
    14821 gaaatgtttt gattaaacat ggttcaaggg aaaaaaagct aatagcaatt acctttgatg
    14881 atggtcctca tcctaaagaa actagtcaag ttttagatgt attaaagaaa tacaatgtaa
    14941 aagctacttt ttttatagct ggaaaacatg caaaatggta taaagaacca ttagtaagag
    15001 caagtaaaga aggtcacgag ataggaaatc atactttcaa tcatcctgat ataagtaatt
    15061 taagttctag tcagatagaa gaggagattg ttaagtgtga agacattttg aaagaagtaa
    15121 ctggaaaaaa acctacatta tttagaccac catttggaag ttatagagaa aaagacttaa
    15181 tagaaattgc aaaaaagcat gactataaag ttgttttatg gacaggtgta gatgtgaaag
    15241 attggaaaaa tccaggagct aatagcattg cagataaaat aattaataaa gtaaaaaatg
    15301 gagatataat acttttgcat gattatgcaa ctaatgatac agttgaagct ttagatatgt
    15361 ttattccaaa aatgattgaa aaaggtttta aatttgtaac agtttctgag ttgataaaat
    15421 aatttttagg ggggagattt acagtatgat tcttatgata gacaattatg attcttttgt
    15481 atacaaccta gtccaatata tagaagaatt aggggaagca gttgtagtaa aaagaaataa
    15541 tgaaatcaaa ataagtgaca ttgaagaact aaatccagag gtaattgtgt tatctccagg
    15601 accgtgttct ccaaaagaag ctggtatttg catagatatt gttgagcatt ttaaggggaa
    15661 aaaacctata ctaggaattt gtttaggtca ccaaactatt ggatatgttt ttggaggaga
    15721 tattataaaa gcacaacagc ctgtgcatgg aaaagtatat agtatcaacc atactaataa
    15781 aggagttttt aggggactta aaaatccttt aaatgtcact aggtatcatt cactaataat
    15841 tgattccaat acagttccaa aagaattaga aattacagca attactgata aaggggaaat
    15901 aatggggatt aggcataaga aatatttaat agaaggagta cagtttcatc cagaagctat
    15961 tttgtcagag tatggtcatg aaatgcttga aaattttata acagaagcta gagaaagggt
    16021 acatatgtaa tatgataaga gagataaata ctaaattaaa ttcttttgag atatttacta
    16081 tttttagaaa tgaacatgac agttttatat tagacagtgc tatggataaa gaaaaattgg
    16141 gaagatattc ttttataagt agccaaccat ttaaggtatt aaagtataag gatactgatg
    16201 aaaatccttt agaagtttta aaagaagaac tacataagta tagggttgta aatgatacta
    16261 atcttccatt tgtaggaggt gctgtgggat atttatctta tgatttaggt aattacatag
    16321 aaaatttacc tagaactgct gtagatgata ttgagatgcc tgatatgtat tttggatttt
    16381 ataatcatgt aatagttata gaccatcttg tgcaaaaaac ttatatagct actccaaaca
    16441 tagatatcga attagaagaa aagataatag atgatataga acaaagaata ttaaaagaag
    16501 agaaaaaagg tatagatagc atatgttatg aagaaaaaga agtaacacct ataagactta
    16561 aatctaattt tacaaaggaa gaatttaaaa atgcagttca aagtgttaga gagtacataa
    16621 gacaaggaga tatatatcaa gctaatttaa cacaaaggtt cagtggagaa acagaactta
    16681 caagctttga attgtatagg gacttaagaa gatttagccc agcaccattt ggggcattct
    16741 taaactttga agatacacat atattatcaa attctccaga aagatttata agatgtgtta
    16801 ataagagaat agaaactaga ccaataaaag gtactcgtcc aagagggaaa gataaagaag
    16861 aagatttaag acttcaacaa gaacttagaa atagtgaaaa agatagagct gaactactta
    16921 tgatagtaga tttagaaaga aatgatattg gcagaatatc taaaactgga agtgttaaag
    16981 ttccagagct atttgtaatt gaaccatatg caaatgtaaa tcacttagtt tccacagttg
    17041 taggtgagtt aaaagatgat aaagatgcta ctgatgtaat aaaagcaact tttccagggg
    17101 ggtctataac aggagcacct aagattagag caatggaaat tatagacgaa ttagagccaa
    17161 cgcaaagaaa tgtatatact ggttcaatag gttatatagg ttttaatggt gatatggatt
    17221 ttaatatagc aattagaaca ataatcaaaa atgacaaaaa agtctatttt caagttggag
    17281 gaggaatgac ttgggattct gacccagatg aagaatatca agaaacactt gataaagcta
    17341 aatccataat gaaggctttg aggggatatt atgaagaata atgtaggttt tgatagtagt
    17401 ttaagtaaat ttggaattgg tttgtttgaa actattagag ttaaaaatgg aatagcagta
    17461 gatttagata tccatataga aagaatgcta agttcaataa attgtttgga tttagatata
    17521 aactataaaa aagatttctt gataaatgaa attgttacat atataaaaaa agaaaatgtc
    17581 attaataaag cacttagaat tactgttttt gatgaaggtt ataatatatc tattagggat
    17641 attccatata atcaagagac gtatgagaag gggtttaaat tagctatatc gccaatagtt
    17701 agggggaata gtttaattca tagacataag actactaatt attttgaaaa catatatacc
    17761 aaaaatatcg caaataagac tggttataat gatggtatat ttttaaattc agaaggtgta
    17821 atattagaat gttctatgag caatattttc tttataaaag gagaaagagt gtacactcct
    17881 agtgatagat taccgatatt aaatggaata ataaaaaaaa ggattataga gatttgtgac
    17941 gaattacata tagagttgat agaaaatgaa attaatatat cagaaattag tagttttgat
    18001 tttgtttttg tcacaaatag tcttatgggc gctataaagg ttacagaaat tgacaaaata
    18061 aaatttaata aagaaaatgt tgtttttgat aaaatagtga agttattaga ataaattaaa
    18121 atattaacaa tttgttggag acaaaagatg ataggttgta tttatgaagt atacaatgaa
    18181 gagatgacta aaataataga aaaccaaaac actttggctg tatatttagt aggttctagt
    18241 aaagacgtag attttactct ttatgatgta gagataaacg atattgatgt gttcgttttt
    18301 gtaaaagaag gggaaaaaca agagagaata ctgtttaaaa aaaagggaat agaatttgat
    18361 gtaaattact tttcaaaaga tggtgtaaaa aaattattat caaataggga atatttcttt
    18421 gtgaaagaaa tgaaagatgc taaagtactg tttgataggg agaatatatc aaatattata
    18481 aaagatataa gtagagatat ttacttagaa ggacccagta aaatgtcatt agaggaaaag
    18541 tcttttataa agcaagatat tggagcaaaa atatcgaatt taaaaaataa agaaaaattt
    18601 gatgtttttg aatatcattt tttgacgaat ttatatttaa aagatataat aattgggtac
    18661 tttaatataa atgataaatg ggtaccaaaa gacaaaaaat tgctaaaagt tttaaaaaaa
    18721 gaaaataatg agctttttga actagcgtca aaagtaagtg aaaattatga ctatcaaaga
    18781 cttttagatg tttataatta tatttttaaa gaaattgaga caaaagaggt tataaaacta
    18841 atcttctagt cacaaatcaa tggggggagt ttaaatgaat aaagttgata aagaaaagat
    18901 acaacatgca gtaagagaaa tactagaggc aattggagaa gacccagata gagaaggtct
    18961 aatcgagact ccaaatagag ttgctaggat gtatgaagag atattctctg gtcttagtga
    19021 agaaccaaga gaccatttaa aagttctttt tgcagatgaa aaacatgaag aattagtatt
    19081 ggtaaaagat attccatttt attcatgttg tgagcatcat ctagttcctt tctttggaaa
    19141 agctcatatt gcttatttac caaaaggagg aagacttaca gggctttcta aacttgctag
    19201 agtaatagat acattggcaa aaagaccaca attacaagaa agaatcacta aaaatgctgc
    19261 agatataata atggaagaat tacaacctta tggtgtctta gtagtagtag aagctgaaca
    19321 tatgtgtatg acaatgagag gtgtaaaaaa accaggttca aaaactgtta catcagcagt
    19381 gagaggaata tttgaaaagg acattgcgtc tagagccgag gctatgagtt tgattactat
    19441 gaagtagcaa tttattatag atataatcat ttattgagat ataaaattta atttaaggga
    19501 gtgtcattaa ttgtgtttga ttatggtaaa agaacttata taatgggaat attaaatgta
    19561 actccagata gtttttctga tggaggagat tttaataact tggacatagc aatacaacat
    19621 gcaaaagata tggttgacca aggtgcagat ataatagact taggaggaga gtctacacgc
    19681 ccaggtcata gctatgtaga ctcagacgaa gagctaagaa gagtaatccc tgtaataaaa
    19741 aaacttaaac aggaattgga gataccaata tcaatagaca cttataaggc agatgttgca
    19801 gaagaagcat taaaattagg tgtcactatg gtaaatgatg tttggggtct tagaaaagat
    19861 aaaaatatgg cttctgtaat aggaaaatat gatgcagaag tgtgtataat gcataatcaa
    19921 gatgggacaa actatgacaa agatataatg gagtcaataa aagacttttt taaggtaagt
    19981 atagaaatgg caatgagttg tggtgtaaaa aaagaaaaaa tagtacttga cccaggcgta
    20041 ggatttggca aggattttga acaaaatatt gaagtattga gaagattaaa tgaattaaaa
    20101 gatttagggt atcctatttt gcttgggaca tccagaaaat cagtaatagg aaaagtactt
    20161 ccagttgagc ctaaaaaaag attagaaggt acaatagcta ctactgttct tggtataaga
    20221 gatggcgtag atatagttag agtacatgat gtttatgaaa atttaatggc agctagaatg
    20281 actgatgcaa tatatagaaa gtaggtataa tataatggat aaaatattac ttagtaatct
    20341 aggattctat ggatatcatg gtgttcttaa agaagaaaat tttctaggtc agaaattctt
    20401 tgtcgacatg gaactttata ttgattcaag agaagcaggt cttagtgacg atataaataa
    20461 atctgtaagc tatgccgagg tttacaatgt tgttaaagat attactgaaa ataaacaatt
    20521 taatcttttg gaggctttag cggaaaatat tgctgaagaa gtcttgaata aatttatact
    20581 tataaatgga gttatggtca gagttagaaa gcctgaggct cctgtaaatg gtatttatga
    20641 ctattttggt gttgagataa ggagaacaag agatgaataa ggcttatttg ggaataggta
    20701 ctaacatggg tgatagattt gataatttat caagagcatg tgaacttttg aaaaatagtg
    20761 attctatata taaagtaaag gaatcaagtt tatatgaaac aaaaccatgg ggatatacag
    20821 aacaagcaga ttttctaaat atgtgtgtag aaatagaaac agaatttgaa ccttatgagt
    20881 tgttagaata ttgtcaagaa atagagagag aactacacag agagagaata gttcattggg
    20941 gaccaagaac tatagatgta gacatactat tttttaatga tgtagcttcc actgatgaaa
    21001 gactaacaat acctcatccg aggattcaag atagagcttt cgttttgata ccacttatgg
    21061 atttaaatga agaacttata ataaatgaaa aaacagtaaa agaacattta aacctcctat
    21121 ctgctgaaga aagagaagaa gtaaaggagc ttgtaggtta tgaaagaaaa cctatttaat
    21181 aataattcaa agatagagat aaatacaaat aacaaaaaga taactgtaaa aaaagataaa
    21241 cttataatag ctggaccatg tgcaatagag agttatgagc aacttttgga gactgctaaa
    21301 tttgtaaaaa gtcagggagc aaatatttta agaggaggag cgtacaagcc aagaacatcg
    21361 ccaaattcat ttcaaggatt aaaaaaagaa ggtcttaaaa tattaaaagc agttaaagac
    21421 gaagttggta tggctgttat tacagaactt atggatgtaa gagatatcga cgaactgtat
    21481 agtataagtg atataattca aattggttca agaaatatgc aaaactttac tcttttaagt
    21541 gaagtgggaa aacaaaataa acctgttatg ttaaaaagag gaatagcatc cacaattact
    21601 gaatggatag gtgcttctga atatatagca atagaaggaa atagtaacat tataatgtgt
    21661 gagagaggta taagaacgta caatgattat actagaaata cattagattt agctgcggta
    21721 cccattattc aaaaggaaac aggtcttcct gttgtggtag acccaagtca tgccactgga
    21781 gttagatatt tggtaaaacc tatgtcactg gcatcttttg catgtggagc agatggaata
    21841 atggtagaag tacatcctga cccagaaaat gctttgtcag atggtgctca atctctttgt
    21901 tttaatgaat ttgaagattt aatgaaatca attaataatt attaaaagag gcaagagcct
    21961 cttttttatt cgtaatcatt gctaaggtaa atttataagt tataatttga tagttagatt
    22021 atttaatata aataaagatt taatattgat agaattaata taaaaaataa aaattttttg
    22081 attaataaga aggatatcaa taaaaaaagt cgaattgtgt atattgagtg attgacaaat
    22141 ttagaatata gtgtagaata gaaatgttaa cacaaaaaat tacaccagaa ggtgataata
    22201 tgaatgatat aaaagacatt attgaagaaa taaaagctag atgcgatata gcaagtataa
    22261 tatctgaata tatgaatata aaacaatctg gagctaatta caaagggtta tgtccatttc
    22321 atggtgaaaa gacaccttct ttttatataa atacatcaaa acagatttat aagtgttttg
    22381 gttgtggtga aggtggagac gtaataaatt ttgtcatgaa aatggaaaat ttggatttta
    22441 tggatgctgt aaaaatttta gcaaataaat gcggtataga aattaacact aacatgaatg
    22501 aagaaactag gattaaaata gaaaaatcta agaaatttca agatatccat acggaagcag
    22561 ctagatttta tctttcaaac ttattgggaa gcaaaaatct tgggtatgaa tatttaagaa
    22621 taagaggact agatgataaa attattaaga aatttggttt aggattttct ttggattcat
    22681 ggaattcact tatgaatact ctaataagta aaggatacaa aaaacaagac ttattggatt
    22741 gcggtctaat tgcaaaaaat agagatggaa ctaattgcta tgataaattt agaaatagag
    22801 ttatgtttcc tatatttgac tatagaggaa acataatagg atttggggga agagtattag
    22861 atgattcttt gccaaaatac ttaaattctc cagacacatt aatttttaat aaaaaacaga
    22921 acttatacgg attaaatttt gctagaaaaa atttagaaag taaaactata gtcttagttg
    22981 aaggatatat ggatttaatt tcgctttatc agtatggtat aaaaaatgta gtagcaaccc
    23041 ttggaacagc cttgactgaa caacaaggca ttttaataaa gagatatgcg gacactgcta
    23101 ttatatctta tgattctgat gaagcaggaa taaaagcgac tttaagagct atagatatac
    23161 ttactaaatt aggtatcaat gtaaaagtgt tagacttaaa agatgctaaa gacccagatg
    23221 aatttgtaag aaaatacgga cttagtgatt ataaaaaagc tatggatgtt tctactcact
    23281 acataaaata taaaatagat catcttaaaa aagaatttaa cattcaaaaa gatgaagaac
    23341 gagtgaagtt cgcaaaagaa gcttctaaaa ttataaaaca attgacaagt cctgttgaaa
    23401 ttgattttta tacaaagtat ttaagcaatc aaatagatat taatgtcgag tctataaaga
    23461 gagaagttta tggcaaaaat tacaataagc catataataa caaaaatcaa aagaaaatag
    23521 aagaaaaagt aattgaaaaa gtcgaagtca gacaagacgg aaaacaatta gttgaagaaa
    23581 ctctaataaa aataatgtta gaagacaaaa aaattagaga aatagcgtta cttaaagtag
    23641 aagaaagtga ttttttattg aaagaaagta aagaaatttt aaattacatg attaaaaatc
    23701 aagaattgga caaaataact attgacaaat tgaaaagttt aaacatatcc gaagaatact
    23761 taaaagaatt aaattcaatt tctttgaata gtataaactt agagaacaca aaagaaatag
    23821 aaggaataat aacaaatatc agaaaaaact cattagaaga gcaaattaat agcttgttga
    23881 gagaacaaca agaattagaa aataataatg atatgaaaga ggtagatggt agagttatgg
    23941 aaattgcttt aaaaatagtt gagataaata aaattttaaa gagcttgtag agaaaggagg
    24001 tttgagtgtg gaaaataaat cgaataaaaa agagttaaaa aaggttactg ctaagacatt
    24061 aatagaaaaa ggaaaaaagc aaggttctct gacacttgca gagataatgg aagctttttc
    24121 ggagactgaa cttgataagg atcaagtaga aaatctttat gagactttag gaaatttggg
    24181 aatagaaata acagaaacaa aaaattataa agctgatata gatttttcgg ttgctgatga
    24241 cgatttaagt ataggccact tagatgaaga tgcagaggca atttcacatg atgactcttc
    24301 tgcaatagaa atagaaactg tggatttatc tttaccaaaa gggataagta tagatgaccc
    24361 tgtaagaatg tacttaaaag aaataggaaa aattcctcta cttaaaccac atgaagaagt
    24421 agaatttgct agaaggatgc acgaaggtga tgagatagca aaacaaaggt tagttgaagc
    24481 aaacttaaga ctagttgtaa gtatagcaaa aagatatgta ggaagaggta tgcttttctt
    24541 ggatttaata caagagggaa atttaggtct tataaaagca gtcgaaaaat ttgactatac
    24601 aaaaggatat aagtttagta cttatgcaac atggtggata agacaagcta taacacgtgc
    24661 tattgcagac caagctagaa ctataagaat accagttcat atggtagaga ctataaataa
    24721 gctaataaga gtatcaagac aactacttca agaacttgga agagatccaa aaccagaaga
    24781 aattgcaaaa gaaatggaaa tgacagaaga taaagtaaga gaaataatga aaatagctca
    24841 agaccctgtg tctttagaaa caccaatagg agaagaagaa gatagccatt taggtgattt
    24901 tattccagat gatgatgctc cagccccagc agaggcagca gcatattctt tattaaaaga
    24961 acaaatagaa gatgtacttg gttcgttaaa tgatagagaa caaaaagtat taaagcttag
    25021 atttggtctt gaggatggta gagccagaac tcttgaggaa gttggaaaag agtttgatgt
    25081 aactagagag agaataagac agatagaagc aaaagcacta agaaaactaa gacatccaag
    25141 tagatcaaaa aaacttagag attatttaga ctaaaaagat acatcattga tgtttttgtg
    25201 ataaatgcca tgagataagt gatatctcat ggctattttg tagtaaagat ttaattttat
    25261 ttttgtagtt atttcagtct aatatctata acttggatta aatcctataa ataaagggaa
    25321 ttgaaaggag aattatattt gaaattaaca gatagattat tgaaaatagc atcattagtg
    25381 tcagatggta aaaaaatagc tgatataggg acggaccacg gatacatacc agtatatttg
    25441 ttaaaagagg gaaaagtacc atttgcagtg ctagcagatg ttaataaagg acctcttgac
    25501 aatgcacata aagaagtcat acagaacaat cttttagata aagttgattt acgattgggt
    25561 tctggtatag agatacttga aataggggaa gtagaagaag tgataattgc tggtatgggt
    25621 ggaatcttga taagtgagct tctcgaggct aaaaaagaag tagcacataa tgtagaaaaa
    25681 ttaatattac agccaatgca agcacaagaa gaacttagat attacctttt aaacaacgga
    25741 tatgaaattt tagaagaagt tttagtgaga gaagatttta gaatatatga gataattgtt
    25801 gccaaatata caggtaaaaa tactataata gaagatgaga tatattatga ggttggaata
    25861 aagcttttag aaaacaaaga ttctcttttt aatgatttta tagaaaaaaa gataaagaca
    25921 tatagttcta tagttaataa attagaaggg aaaaatgggg aagctataga taagaagaga
    25981 aaggaaagcg aagttgcaat aaaaaaactt gaaaatttaa taaaataaat aggaggtata
    26041 tatgctatta aagagtctaa ctagaaaaat tgaaaaaaag tatcctctaa acttagcaga
    26101 ggattgggat aatgtaggtc ttatagttgg agattttgat atggatgtaa aaaaggtatt
    26161 ggtatcttta gaagctaatg aagatgttat tgatgaagct atatccaaaa atatagattt
    26221 aattgtgaca catcatcctt ttatatttgg gaaaattaat aaaattaata gtggagattt
    26281 aaaaggcaga cttatccaaa aacttattaa aaatgatata tcactttatt ctatgcatac
    26341 gaattttgat atagcatttg atggtctaaa tgactatttt atggaaatta tggaatttgg
    26401 taattcaaag gttttagata taactaaaag cgaaaatttg tataaattag cagtatacgt
    26461 tccacataac tattcagatg aacttagaaa ggttttaagt aattcaggag caggtcatat
    26521 agggaattat agtgattgta cgttttcaat agaaggtgaa ggacaattta agccattaga
    26581 aggctctaat ccgtttcttg gaagtgtaaa tgatatagaa actgtaaatg aagttaaaat
    26641 tgaaactgta gtaccacaga agcttttagg tggggtaata agttctatgt tagatgcaca
    26701 tccttatgaa gaagttgcat atgatttgta caagttagaa aataaaggtg agatatttgg
    26761 actaggtaga ataagtaaac ttgataaaag tatgacatta gaaagtcttt caaggaaaat
    26821 aaaagagaaa ttaaatatga aacacataag agtagttgga aaccttagta cagacataac
    26881 gaaagtagcc gttgtaacag gagctggttc agaatttgta aaaaaagcta agagacaagg
    26941 tgcagaagta ctaattacgg gtgatgtaaa gtatcatgag gcacaagatg cacttgatat
    27001 tggaatgtgt atagtggatt gtggtcattt tgatacagaa gatatattca aaaatgttat
    27061 gaagagattt ttagatgaat ttagtgaaat tgaagtaata aagagtaatg tatatttgaa
    27121 tccatttagt actatttaga agtgtttgat taaaaaatac tatataaaaa ataaatttga
    27181 gtttaattag ggggataatg atgaaagtag cagtaatttt tcatagtgtt tgtgggagta
    27241 catatttatt agctagagaa tataaagaag tgcttgaaga aatgaatatc gaggtagata
    27301 tatttagagt aagtgacgaa gttgcaaaaa cacttcctca atattatttg ataaattaca
    27361 aagaatacaa agacgaattt gaaagtataa atgtaattaa atctggaaaa gaaatattag
    27421 attatgatgc tatatttatg ggttcaccaa catattatgg aaatgtatct ggaccaatga
    27481 agatgtttat ggatagtttt tctgatattt gggtaggtgc tcctttgagt ggtaaaatat
    27541 ttggttgttt tgctacagca ggttcacaac atggaggagg agaattagct ttgcaagcta
    27601 tgaatatatt tgctcaacat atgggaatga ctcttttgtc agttccatgt tcagtaagag
    27661 gaggttatcc agcgtatgga atacttcata tagcaggaga taattctgat ataagaccaa
    27721 atgatgatgc caagataggt ataagagatt acctaaaaag acttaacata taagaatatc
    27781 atataaaaat acctaaaagt agctgtatat acttttaggt atttttactt aattatatac
    27841 gtgaagcttt accagaaata tggtcaattt ctattttaac aacagttgtt aagttcatat
    27901 ccttctctat gtacttcatg ccttcttcaa agaatccttt agaatatttt tttattattt
    27961 ctacaagagc atttttcttt tcttcatttt ctacagtgca agcttttcca aaaactatag
    28021 cactagaata agtagtacta aatttatctg gaataacact ttcattagca actactaaaa
    28081 aagaaacttt gttgtttttc gatatattat ctaacttatg tccatttctt gcacaatgaa
    28141 agtaaataga atcattaaag tatacataat ttacagcaac tccataagga taaccatttt
    28201 cagatatagt ggaaaaagta ccaaattcac cattttttaa tacttcaaca gtatcttctt
    28261 ttgtcatttc tctttttttc aatctcattt ctttaaacat aatatcatcc tttctaattt
    28321 tgatgcttaa attaaatttt agcacattta taaagattat gctatgtttt ttacaaattt
    28381 taagaaaaaa atatagttta agataaaagg tagtgaaaga gaagctattt ggaggtttat
    28441 ttatgatatt attgttagag tagtacaaac aatactaata aatttaatag ctggtttctt
    28501 gttatttaag gtagtaagca tgtctataag aaggggaagt tatcgagggt agaagttgtt
    28561 atttataacg atattataaa gaaaatatta ttatataaaa taataaatag taaagaggta
    28621 tcaagttgaa tataatactt aatacctctt caaaattaaa ttataaaagt gttgtgggaa
    28681 ttgtcctata catggtaagg agataataaa gaatgtatct ttaaagtctc aactaaacag
    28741 caaaatttat ccaacaaaca tacaataaat gcttctttgt atctaggtgg cttaacagta
    28801 agtggccaca tgtgttttaa aattatgtcc gtttcttttt catttatttg aaaaaataaa
    28861 gttgcatttt ttaatgcttc tctagggtga gtaaatccat gtaatccttt tctatcacct
    28921 ttgtaatgcc aatcataaag aaaaaagtcg tgtagtaaag ctcctctaat aacactttga
    28981 atatcaacac gtaagtgcaa ttttttacat aatagataac tataatatgc tactgacaaa
    29041 ctatgttcaa gacatgaaac attgccatga tgaggaaatg ttctcataag ttgaactttt
    29101 tcagaagaaa gtatctcaga agcacattca ttaaaatatt tgatttcagc ttttgacaac
    29161 attatgaaaa tctccattct tagtaatata taaatactat acttcaacga taatatgaaa
    29221 tatttcttat cctacaaaat ataataccca ataatacaag taagatacct tttatggtat
    29281 tttaaagtaa gagagatttt attaggctaa acaatgaaaa aattaaaagt aaagctatag
    29341 caatatatag tatttttcca agaggattgc caaaatttat agtccatcca ataccaaacc
    29401 ttttattaac cattaatgat gggtcatttg gattattata catgatgcct gctatccaat
    29461 acttctcatc ttcttctgga gaataagatg agttgaattt taaatttggg gaattaatat
    29521 atgttgctat taaataaatt ataactaaaa gcgttgtaat attcatagca gttgatagtt
    29581 ttgtttttat tgataaaaga gtagtaaaaa actgtgttgt cattataatc atcaatatta
    29641 aaaacaaata accaatttta tttaaatatt taatgttttc aactctactt tcttcaattc
    29701 tatttgtatc tatcttaatc cttgacttta tggatccaat ggaagttata tataataaaa
    29761 ccattaaaca aaagtcagta ccgattaatt taaatacatt aagaaatgat ttatctataa
    29821 aaacatcagg agacccattt atgttccaat gagtggcaat tgagtctggc aatttattat
    29881 aattaaatgc tataaaaata ctcatcccca aagtaaatag tatcggtatt aaatagatta
    29941 ttttaaattt ttttattatt ttatttttct tatttataaa atccatatct actattaatt
    30001 ttgaatctac atctatatga cctaatttag aataaatttc t
//
LOCUS       DAEPOZ010000020        29626 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.020, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000020 DAEPOZ010000000
VERSION     DAEPOZ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29626
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.020"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..764)
                     /locus_tag="KR440_000747"
     CDS             complement(<1..764)
                     /locus_tag="KR440_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HBH0697810.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKSHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKSIIQLIKNISENLNMN
                     PKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKEGK
                     SYEFIKRLLRDFKYVYYYGIENCFIEEYFKYTDKSI"
     gene            complement(817..1176)
                     /locus_tag="KR440_000748"
     CDS             complement(817..1176)
                     /locus_tag="KR440_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBH0697811.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            complement(1230..2315)
                     /locus_tag="KR440_000749"
     CDS             complement(1230..2315)
                     /locus_tag="KR440_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBH0697812.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKVKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANVFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            complement(2486..3865)
                     /locus_tag="KR440_000750"
     CDS             complement(2486..3865)
                     /locus_tag="KR440_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBH0697813.1"
                     /translation="MINIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGVLLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            complement(3858..4538)
                     /locus_tag="KR440_000751"
     CDS             complement(3858..4538)
                     /locus_tag="KR440_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBH0697814.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            complement(4930..6009)
                     /locus_tag="KR440_000752"
     CDS             complement(4930..6009)
                     /locus_tag="KR440_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0697815.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKATEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     regulatory      6297..6412
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6587..7957
                     /locus_tag="KR440_000753"
     CDS             6587..7957
                     /locus_tag="KR440_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0697816.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYALGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDISGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     gene            complement(8222..8833)
                     /locus_tag="KR440_000754"
     CDS             complement(8222..8833)
                     /locus_tag="KR440_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBH0697817.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(8823..11186)
                     /gene="lon"
                     /locus_tag="KR440_000755"
     CDS             complement(8823..11186)
                     /gene="lon"
                     /locus_tag="KR440_000755"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBH0697818.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFNSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLGNIPRPLLDRMEVIEVSGYIEEEKLNI
                     ARKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTAVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            11366..11878
                     /locus_tag="KR440_000756"
     CDS             11366..11878
                     /locus_tag="KR440_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBH0697819.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            11866..12393
                     /locus_tag="KR440_000757"
     CDS             11866..12393
                     /locus_tag="KR440_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBH0697820.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(12464..13714)
                     /gene="clpX"
                     /locus_tag="KR440_000758"
     CDS             complement(12464..13714)
                     /gene="clpX"
                     /locus_tag="KR440_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBH0697821.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(13733..14317)
                     /gene="clpP"
                     /locus_tag="KR440_000759"
     CDS             complement(13733..14317)
                     /gene="clpP"
                     /locus_tag="KR440_000759"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBH0697822.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            complement(14466..15752)
                     /locus_tag="KR440_000760"
     CDS             complement(14466..15752)
                     /locus_tag="KR440_000760"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBH0697823.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            complement(15946..16416)
                     /locus_tag="KR440_000761"
     CDS             complement(15946..16416)
                     /locus_tag="KR440_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBH0697824.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTNMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            complement(16416..17765)
                     /gene="rph"
                     /locus_tag="KR440_000762"
     CDS             complement(16416..17765)
                     /gene="rph"
                     /locus_tag="KR440_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBH0697825.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEIADEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARAIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNVPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            complement(17925..19949)
                     /gene="ligA"
                     /locus_tag="KR440_000763"
     CDS             complement(17925..19949)
                     /gene="ligA"
                     /locus_tag="KR440_000763"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBH0697826.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            complement(20135..21073)
                     /locus_tag="KR440_000764"
     CDS             complement(20135..21073)
                     /locus_tag="KR440_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBH0697827.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            complement(21175..21597)
                     /locus_tag="KR440_000765"
     CDS             complement(21175..21597)
                     /locus_tag="KR440_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HBH0697828.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSEKNGENIIRDILDGNISSECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            21785..23068
                     /locus_tag="KR440_000766"
     CDS             21785..23068
                     /locus_tag="KR440_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HBH0697829.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSHSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            complement(23146..23661)
                     /locus_tag="KR440_000767"
     CDS             complement(23146..23661)
                     /locus_tag="KR440_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBH0697830.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(23661..25037)
                     /locus_tag="KR440_000768"
     CDS             complement(23661..25037)
                     /locus_tag="KR440_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBH0697831.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            complement(25062..25613)
                     /locus_tag="KR440_000769"
     CDS             complement(25062..25613)
                     /locus_tag="KR440_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBH0697832.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            complement(25671..25844)
                     /locus_tag="KR440_000770"
     CDS             complement(25671..25844)
                     /locus_tag="KR440_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697833.1"
                     /translation="MCTKKEEQKCSKCEICGVLIDDTDKVYNNTYNLCKKCKRNISTE
                     KIVKYINIYDESK"
     gene            complement(25855..26688)
                     /gene="fdhD"
                     /locus_tag="KR440_000771"
     CDS             complement(25855..26688)
                     /gene="fdhD"
                     /locus_tag="KR440_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBH0697834.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYVNTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCASVKSNIKINYDTIYSIMDRNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            complement(26737..28881)
                     /gene="fdhF"
                     /locus_tag="KR440_000772"
     CDS             complement(26737..28881)
                     /gene="fdhF"
                     /locus_tag="KR440_000772"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(28465..28467),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HBH0697835.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKTWGVKLSPNNGYSLTQVPDLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            complement(29132..>29626)
                     /locus_tag="KR440_000773"
     CDS             complement(29132..>29626)
                     /locus_tag="KR440_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBH0697836.1"
                     /translation="FMRITIKHLSKIKFLKFLDNKIESIESFIDDYSKAINFFAKNKK
                     VLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFLYMAISPIPTPGNVG
                     ANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVITHYRMKNRIGRNIV
                     LSEN"
BASE COUNT         8870 a         5341 c         3227 g        12188 t
ORIGIN      
        1 tcaatagatt tatctgtata tttaaagtat tcttcaataa aacaattttc tataccatag
       61 taataaacat acttaaaatc ccttaataat ctttttatga actcatatga ttttccctct
      121 tttgatagca agtatgtttc tcttattttt ttatcttttg ttatatttat aattaactga
      181 ctttttttac attctataaa taccccttct tctgaaaaat tctctattaa atttaagttt
      241 ataagctttt gtaatatatc aaaatttata tttatagtct ttggattcat atttagattt
      301 tctgaaatgt tcttaattaa ttgaataata ctctttggga aaagaattac ttgtatattc
      361 aatttatcat ttttagaatt tataacttta tattttattg aatagttatt tatgtttatt
      421 ggaatgtatt gtgttatttc aaattcaatc atgcttaata tgtctctttt tttcttagta
      481 tttagaactt caacatttct aatgactata tcttgaattt gaatgttaaa gtataaattt
      541 ttatataata tttttgattt accaatctcc tcttttacaa atctaactac atcttctata
      601 tatttaatct tctcaatcga attactatca ttttttatat caaagttaat ctcttttata
      661 attgatttat ctataaaaac ttttttatga ctttttctta aaaatacaag ccttatataa
      721 tcatcattta tttctaagtt tagaattttt ttaattatag ccatctcctt cttaaataaa
      781 aataatttta aaacttacag caatcaatat aaaatattat tttggataaa attgtttaga
      841 atttatattt acgcttatat ctgttccgct atcatttatg acaagttcga atttacctga
      901 tgtactctga ggaactggaa cagtatttag gtatcccttt tccttcaatg tttctacact
      961 tagagagtct attgtctttt catcatcatt aattgccatg ctagctgcag ttactatatt
     1021 agcagcattc gtatagtctg catttaattt ggcactttct tgaaccttac tatacttaac
     1081 taatgctata ctagatataa cgcctaaaat agttactact acaatcattt caacaagtgt
     1141 aaatcctttt cgttttttat ttatcaaatt tatcaattaa atattctctc cttttcaaaa
     1201 gtttcatcta ataaaattta tttttctctc taaaaattag taattgcatc aaacatagga
     1261 acaaccattg ctaaaataaa agtgccaata attaatccca taaatacaat tagcataggt
     1321 tctataaatt tcattatttg ctctattttg ttatttaatt cattttcata aaatttattt
     1381 attacattta gacttttatc tagtttccca ctttcttctc ctgtatttgc cattgaaata
     1441 aataattttg gaaaaacatt tgctaaattc aaagatttac ctatgctatt tccttttttt
     1501 atataattat tagaaatctt taacctttca taaataaatt cgttgtctat aacactagct
     1561 gacatgtcta tagcttccac aatttgtatt ccactgctaa ttaaaacgga caatgccctt
     1621 gaaaatcttg ttgtagctac taactgatta atttttttta taaaaggtat tttgaatttt
     1681 aacgaattaa tcaatttttt tatattatca tttgttataa agaaataaat gcttcctatt
     1741 gaaaacaata taaaactaaa tattatataa gctaagttat ttgtaaccac tgtagatatt
     1801 cccattaaaa ctctagttat taaaggtggt tcaattccat tgttggtaaa aaccacctga
     1861 aaattaggta ttacaaatac gaatataaac aacattgata agatagataa tattataagt
     1921 attattggat atattgaaat agttattatt ttcattttta atttgtattc tttatcataa
     1981 taattagata aatcactcat tattatatct aagtttccac tagtttctcc tgcttttatc
     2041 atacttataa agaatttaga aaatatacct gttatttgaa atgattctgc tattgagtta
     2101 cctttttgaa tatgatttga tactatacta agcaaattct ttactttttt acttgactga
     2161 ctctcaatga tttcaaatat ttttgtaatt tcacatccag attccagcaa tatgcctatc
     2221 tctttgcaaa ttactctaat ttctttatca ctgattctat ttaatactaa ataaaacttt
     2281 tctaaattat aaaatatatt tttagataat ctcatgtact ataatacctt ccaaattata
     2341 ttaattacaa ttaaataaac ttaatatttt ctaatttaat gcatttaatt ttatttcact
     2401 taaatataat ttatactcaa acatcttaat taaaatagat taaataaaga caactgacca
     2461 aacgaagttt aactatacaa ttcagttaaa tcatttcatt tactactaaa cactcttcta
     2521 atgtcgttat tttttcattt attagtcttt tacagctatc ttcaaatgtt atcattccat
     2581 tattttgtgc cacatcttta atagtactag aatcctccat atttcttata catgtcttta
     2641 tatcatcatt aatttctaag atttcataaa ttgctgttct tccaaagtat cctttatcat
     2701 tacatttttc acaccctaca gctaccttag tatttacatc tccactaaaa ttatctttta
     2761 tcataatctc atggctacaa tgattacaaa cttttctaac caacttttga gatataacac
     2821 ctataagaga ggcatttaaa agatatggtg gtatttgcat ttctagtaat ctagctatgg
     2881 aagatactgc atcattagta tgtaaagtac ttataactaa atgacctgtt atagctgctc
     2941 ttactgctat cttagctgtt tctgcatctc ttatttctcc gaccattatt atatctgggt
     3001 cttgtcttaa tatcgctcta agacctacat caaagctcaa ccctactttt gaatttactt
     3061 gaatctgact tatgccatcc atcttatact ctacaggatt ttctatagtc ataatatttt
     3121 tatttattcc ttttaaatca tttaataagg aatatactgt tgtagtcttt ccactacctg
     3181 taggacctgt cactaataat actcctgctc ttttatttat tatacgattt atagatttta
     3241 tagcttcctc tgaaaaacca agttctaatt tgtcttttaa aaaagtatct ctattaagta
     3301 tcctcaaaac tattttttca ccatagacag ttggtattga tgaaactcta atgtcaatta
     3361 aatttccacc taactttata tccaccctac catcctgagg aagccttttt tctgttatat
     3421 ccattgatgc ctttaatttt ataaccgtag acaatgatgg atagacatca actaaaaact
     3481 ttgatacttc ctttagttct ccatctaccc tcattctcac tattaaatgt tctttaaatg
     3541 gttctatatg tatatcactt gcattttctt ttatagcttt ttctaaaaga ttttcaaata
     3601 atttttttgc ataatcctca tcaagttcaa agctattata atatttttca atcttttcac
     3661 ttataagttc ttttttctcc ttcttcaata taatttcttt acctgtagct aatctcaagt
     3721 cctgtagagc ataaatatcc tcatcttcta tttctacaac taaattatca tcttgaaact
     3781 caactggaaa tacattatat ttctttgcaa gtttttctgg tattaacatt ggcaaactgc
     3841 tttcctctaa tataatatta atcacccttt atatacttta atttattagg tatattcatt
     3901 tttttcatag agatattttc ttcagttttt atgcttattt tcattggata attggtttgt
     3961 atgctatcta aatatttact acctttatct acataattag ataatttctc cttatctcca
     4021 atgcacttta tttcatatgg ttgtgctaca ggaactccat ttatatttat atggcttcct
     4081 gcaagtacaa cttctgaata ttgattaatt ctttgaccat ttatttctat gtattttcct
     4141 ccgtttactt ttaattcatt tataactttt acaagaatct tattatagtc tatactatta
     4201 gctatattcc ctgttttttc atctatagca tctatactta atattatccc actacccttt
     4261 acatctgaat atgatagact atctctaagt gtttctattt ctgatatttc ttcagaattt
     4321 ttttgatttt gtttcattga attaatttct ttatctaact tgtctttctc tttggttaat
     4381 tcttttatag attttcttgt catttttatt tcttttctta ttgacttgtc tttgcttgta
     4441 taaactgttt cttcattgct ataatctcta aatatggttc caacaagtat acctaaaata
     4501 gtacttgcta gtattataaa cacatattgt tttttcatac tgtattgctc cttacaaatt
     4561 tatttttata tgataatttt ctttaattaa atattaaaca tttacatatg cttattatat
     4621 caaagttata tattaaaatt catctttagt atataatatc atacattgaa ctcctatcac
     4681 taatttacca atatcaagta tgcattttta gatgttctta aaattatata aactttcaat
     4741 aaacattgtt gtgatattat aaaaaaacat aaattaggaa aggaaataat aattatgaaa
     4801 aacaaataat ggctttggag attattaata tcatttttta gcaaaaaaaa gtaaaatggc
     4861 atagcctcaa agctacacca atttactaaa aattatatta ttattaacat taattttaaa
     4921 catatacgtt tataagtttt cctcatattt taaaattctt ttaattaact tatctcttaa
     4981 tttccctact tcatcaaatc tcatattagg actatagtaa ctttctataa tatcatgttt
     5041 agacttggct ctcttaagat ttagtactgc ccaacaaatt aaatcatcta aaactttttc
     5101 atcaaattta atatcttctt catatttaag tagtttttct ctatttaaaa actgatttaa
     5161 atcaattgta tcttcttctt taaaagtcgt acttatagtt atgcctatat ctaaatcagt
     5221 taatagtaca gtttcaattt tctctggaat aagtggataa tgataaactt ctacatttaa
     5281 acctttttca gtagctttct tatatacttt gtttaataat gtagttttcc cagttcctat
     5341 ttctccatct aaataataga ctttctttac atcttttaaa atgttttctg tataatctaa
     5401 acaccctaca ggagttatag ctgaaccaaa taaatgcttt tctcttttta gatttcctgt
     5461 gcttttaact ccatcaaata acctttttat aaattcttct gtgaccaaat ttactttgcc
     5521 ataatccata caatcagaat atttttcttc tatgtctcta aaaattggtt ctgctgactt
     5581 taaaaactta aatgctcttt taaatgaatt actaatatct ttatcataaa ctgatatttc
     5641 ttgtttattc ttttctaatt cttctgtatt ccagtaatca cctaaattaa ttatctcatc
     5701 caaagctcca ggtagctttg ggtccatagt atgtggggca gttgcatcca taagtaccac
     5761 gcctaatttc tttataacta aaccatccaa cgaacttggg tcagaaggac aatgatataa
     5821 atcaatatca tatcctctat gcaaaaactc tttataaatt tttttcataa gtgaggattt
     5881 tcctactcca ggtccaccct ttaaacaaaa aactcttttt acattttcag gaattatata
     5941 gtcaaaaaaa gaatatgagc cttgtgatgt atttccacct gggaatgcct tctttacctt
     6001 tccttccata ttagcgcctc ctcataattt acaatatctc ttaattaaga tattcatatt
     6061 ataagaattt tattacagct ttctgatttt gacaaattta aaacttacca tctaatacta
     6121 ataacatatt aattttatct aattattata gattctcata ctgtacctta ttctatctat
     6181 aaatacaatt taagtgtcca tattgaaata tttgtattgt aatacagctg gatattactt
     6241 aaatccaatt gtttccatta taattttatg ttaaaataat attacaaaat acatctgttt
     6301 ttcttcataa acgggtgaaa ttccctatcg gcggtaaaag cccgcgagcc ttatggcata
     6361 atttggtcat attccaaagc cgacagtaaa atctggatgg tagaagaaaa tagtatatga
     6421 gtacctttat gtaattttac atgagtaatc tatacaaatc cttcaactac cgtatttatt
     6481 catgaaatta gacacattca aggtacctaa tatacaggtg ctttttttgt tgtttatttt
     6541 acaattatat cgtacttata aaatctatta agattggagt gttatcatga aacaaaaatg
     6601 gatagtattg attatcatct gtattggtgt atttatgtct actcttgatg gaagtatact
     6661 aaatatcgca aatcctacaa ttgctgctga ctttaaaatt aacatgtcac aaattcagtg
     6721 ggttgtaaca gcttatatgc ttgttgtaac agctactatg ttatttttcg gaaaacttgg
     6781 tgataaagta ggttcaaatc gtctatacgc attaggattt tttatattta caataggttc
     6841 ttttctgtgt agtatgtcaa acaatttatc aacattaatt tcttcaagaa tctttcaggc
     6901 agttggagca agtattttaa tggctactgg gcttggtata gtttccaatg catttccagc
     6961 aaatgaaaaa ggaaaagcta taggaatcac tggagctgta gttggtatag gaaatatgag
     7021 tggtcctgta attggaggta taattttaga acattttgga tggccttcta tttttattat
     7081 caatatacct attggtatta tagcagtctt tcttggcatt aaatttctgc ctaaacctgt
     7141 tttggatgaa caaaacaaga gctttgatat ttcaggtctt ctacttttcg catcttgcac
     7201 tacgcttata ttacttgcaa tgaatgagaa gggtaataca agattgtatt taggtataac
     7261 agcacttata atcttcttat tgttggcatt aagagaagta aaatttgaac aaagctttat
     7321 tgacctacct ctatttaaaa atagaaactt tactgtaggc aatatcattg gagtagcttg
     7381 ttattttcct caaatggcag tatcttttct acttccattt tatttagaac aattaaaaaa
     7441 tttatctcct atgatggctg gttatgtaat gactgtacat ccacttatta tggtacttat
     7501 agcacctatt gcaggttcac tttctgataa acacggtgca aaaaacattc taacagcatc
     7561 tttttcattt atgaccatct cactagttgg aatggcactt ttaaaagcag attctccatt
     7621 gtatctttta attgtatgtt tagttatttt tggacttggg ctaggagcat ttagttcacc
     7681 aaacaatagt agtattttag cagatgttcc tcctcaaaaa caggggtacg gaggcagttt
     7741 tcttgcaact attcgtaatt tatcatttgc tcttggaaca gcatttttta gtagtttttt
     7801 tgcacaaagt cttacataca atcaaaagtt taaaagtcac acatcagcct atgttatagc
     7861 tagcaatcag tcttattgga ttgcagcatc agtatgtttt atagggctta tactaactgt
     7921 tttctttatg agaaaaacag ataaatctat ttcttaaatg ctatttattg ctgactacat
     7981 aagtcaataa ttttttagtt gaactaattt atactaattt tcccatacct tactaaaatt
     8041 tattagtatt tagcaaaatt actgatattt attgtttata aacaataagg atgccaaaaa
     8101 aagttcatta aaactatttt aaggcatcct atattttgct ttaatattaa tataattaaa
     8161 actaactatt attacattta ttattcaaat aaatcttatg tcaatattta acattttaaa
     8221 tttaatctac tgttatttca tatccatgtt gattgtattg atttacaatc tcttcccata
     8281 attcttcaac accagtcttt tttagtgaag aaacaggaat tactttttca ctactttcca
     8341 tttgcaattt ttttcttatt atacttatgt gtttttgata ttgtcctcta gatattttat
     8401 ctgccttagt tgcaataact acacagttat atccaaagtg ctttatccat tcatacataa
     8461 gcttatcatc tgctgtaggc tcatgtctaa tatctacaag taggaatata gaacataact
     8521 cttgtctact ttctagatat ctttccatta tacctcccca cttttctttt tcagacttag
     8581 ctatctttgc atatccatag cctggaaggt ctacaaaata aaattcatta tttattagat
     8641 aaaaatttat agttctagtt tttcctggtg tttgactggt tctagcgaag tttcttctgt
     8701 taagcaatgt atttattatt gatgattttc caacatttga cctaccagcc aatgctatct
     8761 ctgggattcc ttctgctgga tactgtgatt tattcacagc actcatagtt atctctgaac
     8821 ttctaatttt cattattttc accactcttt aataaagctt gttctaatac ttcatccata
     8881 tgttctacaa gcacaaactc cattttttct tttacatttt ctggtatttc atctaaatca
     8941 gcttcacatt ctttaggaat taatactttt gttataccag ctctatgagc tgctaataac
     9001 ttttctttaa cccctcctac tgctaagact ctacctctta aagttatctc tcctgtcatt
     9061 gctatatttc caggaacagg tcttttagta agtgctgaga ttactgcaag agccatagtt
     9121 ataccagctg atggtccatc ttttggaaca gcgccttctg gaatgtgtat atgaatatca
     9181 ttcgtcttat aaaattcagg gtctatatca aacttgtcca ctattgacct tatataagag
     9241 atacctgtct tcgcagattc cttcataaca tctcctaatt ttccagttaa tacaatttct
     9301 cctttacctt taagtacgtt tacttctact tcaagtgtaa ctccaccaac tgctgtccat
     9361 gcaagaccat ttactaatcc tatttgagga ttaacttctg caagttgata cttaaacatg
     9421 tcttttccaa gatatgtctc taggtcagac ttatttataa caacctcttc aagagttggg
     9481 tcttcaacgt attttttggc aacctttcta caaatcttac caatagttct ttctagtgtt
     9541 cttacaccag cttctcttgt ataatggtta attatacttc ttaaagtttc atcatctatt
     9601 tttataaagt tttcttttaa agcatgttct ttaatctgtt ttggaagtag gtattttctt
     9661 gctatattta atttttcttc ttctatatac ccagatactt ctattacttc cattctatct
     9721 aataatggtc ttggtatatt acctagacta tttgctgttg taacaaatag tatttttgat
     9781 aaatcaaaag gtatttctaa gtaatggtcc acaaagtcct tattttgctc tgggtctaat
     9841 acttctaaca ttgctgaagc tgggtcacct ttataatctg ctgccatctt atctatctca
     9901 tcaaataaga atacaggatt ttttgtttgg gcttctttta ccccattaat tattcttcct
     9961 ggaattgaac ctacataagt tcttctatga cctcttattt ctgcttcatc tctaactcca
    10021 cccaaagata ttcttacaaa ttttctattt aatgctctgg cgattgattt tactatagat
    10081 gtctttcctg ttcctggagg accaactaga catattatag gccctttaag tgacttagca
    10141 agagttctta tagctagata ctctagtatt ctctccttaa ctttttctaa tccataatgg
    10201 tcttcatcta atatatcttt tgccttagtt atatctaatt tatcttttgt ctctttattc
    10261 catggcaatg agaatatagt atctaaatag tttctactta cagatacatc tggagacata
    10321 gaagatattt ttgaaaattt atctatctct ttttctattt tttctttggt tgtcttaggt
    10381 gcttttattt ttttaagctt ttctctatac tcatctgctt cagaatttat atcttcttct
    10441 tcacctaatt ctttctgtat agcttttaat tgttctctaa gataatattc tttttgaacc
    10501 ttattcatct gtttttttac tcttaaagtt attttctttt ctatttttaa tatatctatt
    10561 tcctctaata atatagaata aataagttca agtctttttc taatatcaaa ttcttctaat
    10621 atttcttgtt tttggcttga ttttagatat atgtttgcag ctatcgtatc tataaatcta
    10681 tctacatctt ctatatcagc tagagatatt aatatttctg gtgataccct atttccaata
    10741 tttatatact cttcaaaggc atcaaataca tttcttacaa atgcttcaat ttcaacttct
    10801 gtttcactat ccaaattatc actattaaat actatttctt ctattactgc tctgaaatac
    10861 ccatcttctt gttctatttt ctttactcta ccccttgata caccttcaac caaaacacga
    10921 actgtatctc ctggtaattt tatcatttgc ttaactttac agattgttcc cacatgataa
    10981 aaatcctctt ctcctggctc atctacctct gcttctttct gagaagttaa aaatataagt
    11041 tcttcatcca tcatagcttg gtctaaagct ttaagagata tctctctacc tatatcaaaa
    11101 tttaatatca tatatggaaa tatagctaat cctcttagag gaattaatgg taattcatga
    11161 tctattttag tataattttg ttccatcaac attactcctt ttctagtcta attgctttgc
    11221 ttttaatatt atatatttga acaagttaat tttcaacatt aatgataaat tcttagaata
    11281 ctatcatact atattatctt tttattatga tataatatta atattagtat acttagatgt
    11341 tatttatctt ttggaggttt ctattatgtt gaaattgttt ttaacctttt taaaaattgg
    11401 tgcgtttaca tttggtggtg gatacgctat ggttcctctt atacaagatg aacttgtaaa
    11461 caaacaaaaa cttttagagc aggaagaatt tattgattat ttatccatag ctcaaagtta
    11521 tcctggagta ctggcagtta atatatctgt attattagga tataaacttt atggtatacc
    11581 aggagttctt atatgtacct taggctctgc tttaccatca ttttgtatag tcttagtctt
    11641 atcgtatcta tactttaaaa attcatcatc taaaatttta gatggatttt ttaaaggagt
    11701 tgcgcctgtt gtaatagctc ttatattgta ttcttttacg agcatgttca agaaactacc
    11761 aaagtctaaa actaacatac tactattaat tatagcagtt ttatctgttg gtgtattcaa
    11821 tataagccct atttatttaa taattggagg aggtgtatat agtctatgtc agaaataata
    11881 ctactcttct taatattcct taaaataggg gcatttagtt ttggtggcgg ttatgccatg
    11941 ctaccattta tccagcaaga atttatatct aaatatcact ttataactaa tcaagagttt
    12001 ttggatttac ttgctatatc acaatctaca ccaggtcctg tagcaataaa tagtgctaca
    12061 tttgtaggat ttaagactgc tggcttcttt ggttctctag ctgcaactat tggtgttaca
    12121 atttttgcat taataggtct aactattata tcaaagttat tttctaaatt taaaaataat
    12181 aaaaaggtag aaaacttatt gacagttctt agaccaatta ctctaggatt catattatca
    12241 gcagcttttt catctgccaa agaagttaat tgggatttat atggaataat tgcatttata
    12301 gcatcatttt atcttttata taaaggaaaa attggttcca ttgcaattgt attcgtcttt
    12361 ggtattctag gtatattact cacattttta tgataatgat acctatttta tatttagcat
    12421 attaacataa aaaaaggagc ttttaaagct ccttttcatc atcttatgca ctttcctctt
    12481 gatccttaag tactatgata ggatttactg agtcttcgtt cacagatttc tctgttacta
    12541 taaccttctt tatattatct ctagatggta cttcaaacat agtttccatc attacgcttt
    12601 caactatact tctaagtcct ctagctcctg tatttctttc tatagccttt ttagcgattg
    12661 cacgaagagc accttcttca aattctagct caacatcatc taactccaat aactttttat
    12721 actgttttac tagagcattt ttaggttcct gtaatatctg cattaatgca tcttcatcca
    12781 acaactctaa agtagctaaa actggtattc ttcctataaa ctctggtatt ataccatatt
    12841 ttaataaatc ctctggaagt actttttcat acagcttccc taaatctaat tcttttttac
    12901 tttctatttt agctccaaaa cctagtgttt tatctccacc acgtttttga attatcttct
    12961 ctaatccatc aaaagctcca cctaatataa ataatacatt agtagtatct atctttaaaa
    13021 attcttgatg gggatgtttt cttcctcctt gtggtggaac atttgcaaca gtaccttcta
    13081 atatttttag aagtgcttgt tgaacacctt caccactaac atctctagtt atagatgggt
    13141 tttcagattt tcttgcaatc ttatctatct catctatata tattatacct ctttcagctt
    13201 tttctatatc aaaatcagca gcttgaataa gttttaaaag tatattttca acgtcttctc
    13261 caacatatcc agcttcagtc aatgatgtag catctgccat tgcaaaaggt acatttagag
    13321 ttcttgctag agtttgagca agtaatgtct ttccagaacc tgtaggtcct aataaaagta
    13381 tattactctt ttgaatttct atatccttac tactagattt tttactgtat attctcttat
    13441 aatgattata aaccgcaact gataaagctt ttttagcttt ttcttgacct attacataat
    13501 catttagaat ctccatcatt tcctttggtt ttggtaaaga tgtagtatct tcatctatag
    13561 tatcttctat ttcttcctga ataatttcgt cacaaagttc aacacactcg tcacaaatat
    13621 atacatttgg acctgcaatt aatcttctta cttgatcttg atttttaccg cagaatgaac
    13681 actttaattg tcgtttttct tcatatttag acatattaac acctctctct atctacggtc
    13741 tctttgtaaa tacttcatct attaaaccat attctttggc ttctagtgca ctcatgaagt
    13801 tatcacgttc agtatccatt ttaatttttt ctaatggctg accagttctt tcagataata
    13861 tctcatttaa agtttctttt atttttaaaa ttctctttgc atgaatttct atatctgttg
    13921 cttgaccttg agctccacct aatggttgat gtatcattat ttcactattt ggtagtgcta
    13981 gtctttttcc ttttgctcct gctgccaaca agaatgctcc catagaagca gccataccta
    14041 tacatatagt acttacatct ggtttgatgt attgcatagt atcatatata gccattccag
    14101 aagttatact tcctcctgga gaatttatat ataaatgtat atctttgtct gggtcttcag
    14161 cttctaaaaa taatagctgt gctactataa gccctgctgt ggcatcgtta acttgatctc
    14221 ctaagaagat tattctgtct tttaatagtc tagaaaaaat atcataagat ctttctcctc
    14281 ttcctgtttg ttcaacaact acaggtacta atgccatggt ttcatacccc ctttattcta
    14341 tataatttac agtagctttt ttaagctact gtaaattatt atacgcattt aactaaatta
    14401 tatttgcaca tcattatata ctaaacattt cataacgtca actatattag ttagcgttta
    14461 ttttattaag ctattgttgc attatcaact aataaatcta ttgtttttct tatttttatt
    14521 tgacctttta tatcttcttt gtcagcatct cttaaagaag cttctatttt ttctacttcc
    14581 atattgtaag cagaagccat tttttctaac tcagccttaa attcttcttc tgttgcttca
    14641 attccttcag ctttagttat tgcttctaat actaaagaag acttaactaa ttttttagca
    14701 tcttcttttc tctcttctct taattcttcc atagttctac cagtcatctc tagtaattgt
    14761 tgtaatccaa atccttgata ttgtaattgg tagttaagtt catttaacat attatctatt
    14821 tgatgttgta tcataacttc tggtatttct atttctgtat tttcagcaac tttttctact
    14881 actgaatttc tagtttctgc atctgctcta tttttagctt cttcttctaa cttagctctt
    14941 acatctgctt ttaattcatc taaagaatca aattcactag tatctttagc aaattcatca
    15001 tcaagtgcag ataattcttt tacttttaca tcatttattt ttacattaaa tacaactggt
    15061 tttccagcta aatcttgtga gtgatattct tctgggaaag taacattaac ttctacttct
    15121 tcaccagctt ttttacctac taattgttct tcaaatcctg gtataaatgt gtttgaacct
    15181 ataactaagt tataattttc acctttacca ccatcaaaag ctacaccatt ttcaaaacct
    15241 tcgaagtcta ttatagctgt atctccatct tctaaagctt tgtcttctac acttactaat
    15301 ctggcatttt tttcaaccat ttcttgtagt ttagcatcaa cattttcgtc acttacagta
    15361 ttctcaacct tagctatttc tatacccttg tagtttccta attcaaactc tggtttaact
    15421 tctacattaa gaaccataac caacccatta tctttactta tttcttctat atctaaatct
    15481 ggattgtcta ttgggtctat gtctaattcc ttgatagctt ctggataaac ttctgggaat
    15541 aatatttcta tagcatcatt gtagaatata ccttttccat attgagtctc tataacaatt
    15601 cttggagctt taccttttct aaatccaggt atattgtatt tatttctgct tttattataa
    15661 gccttattta ctgctgcttc aaatttattg ttatctactg ttatttttaa agtaacttta
    15721 ttaccctctt ttttaattag ttctgctttc atccttctaa tcctcctagt gatatttatg
    15781 taatacccga taacggtatt ataattcatt ctatttataa ttcaaccact taattatatc
    15841 acataagttt ttttatttac aagtgttcca ttaatatcgt tgtacttatt tgataaaatt
    15901 taattttttg agcgtttttt gccatttttt tagataaata cacctttata tgcgtatttc
    15961 ttcaattttt atgtcatctt cttctatatc cataatgaca aaacttttat acgatactcc
    16021 tcttggaagt gaagtgcttc caggatttat gtaaagtaca ccatctttaa tttctctaaa
    16081 tggggtatgg gtatggccaa atataactat atcagcatca acttcagttg cctttttttc
    16141 taacatatta gttccatatt ttacaccata cttatctcca tggcataaga atatggtttt
    16201 atttgccact tcaaagacaa cttcttcctc aacagcatca aagtcacaat tacctttaac
    16261 tgcaataata cctacattag tcatgctatg aatatatctt gaatcagtaa agttatctcc
    16321 tgcatgaatt attaggtcac attctttcaa atatggtatt gttttttcca tatttttaat
    16381 catcatatgt gtgtcgctta ctatgcctat tttcattact tcactaccct ttttataaac
    16441 tcttgtttca taagttttag tgcgtttgct ctatgactga ttgaattttt tataacacta
    16501 ggtatttcac caaaagtctt atcatatcct tttactataa ataagctatc atatccaaaa
    16561 ccatttttac ctttttcttc aaatcctatc attccttcac aaattcctct tactacaaat
    16621 tcttttccat caggaaaaac tactgctatt gcagagacga atcttgcatt tcttttgctc
    16681 attggtacat tttgcatagc ttttaacagt ttttttctat tttcttcatc tgttgcatgt
    16741 tctcctgcat atctagctga ataaacacca ggttttttgc ctaaagcatc tacttctaag
    16801 ccagaatcat ctgatattgc tataagttta gtcttttttg ctattgctct agcttttatc
    16861 aacgcattat gctcaaatgt ttttccatct tctactatct ctatacctgc taaatcaaca
    16921 tcttttaaag agtacacttt atattcaaaa tcttttaaga tttcacctat ttcttctagc
    16981 ttatgagcat tattagtagc aatcactact tcattaccat aatccatacc caatatctca
    17041 tctgctattt cacctaaagc ttctctttga agccttatta attctttatt ccctttttcg
    17101 cctaactcta acaataaatt taaatctgct cttgaaaaag gtttctcttc tcctgtcccc
    17161 tgtacttcta caaactcaca cttatcagtc attattatat tcatatctac ctgtgcttta
    17221 gaatcttctt cataacataa atctagcatg tgtattccat ttacaatacc aacactaatt
    17281 gcagatacaa aatttgttat tggcatctct tttattgtct ttgctttata taatttatat
    17341 attgcttctg caactgctac aaatgcccct gttatagatg cagttctagt tccaccatct
    17401 gcctgaatca catcacaatc tacccaaata gttctttctc ctaatttatt caagtctaca
    17461 acagacctta ttgctcttcc tattaacctc tgtatttctt gtgttcttcc atcaattttt
    17521 cccctagatg catctcttat ctttctttgt tgtgttgctc ttggtagcat agaatattca
    17581 gcatttatcc atccagtacc actatttctt aaaaaaggag gaactttttc ttccactgat
    17641 gcagtgcata taacctttgt ctctcccatt tctataagaa cagaaccttc tgcatacttt
    17701 gtataatctc tagttatctt tacaggtctt atctgttcgt tacttctatc ataacttctg
    17761 gtcatttttt atatcctttc tcatattttt tattttttat aaatttattt ttataatttt
    17821 taattttgtc aaatattaat tattgtcatt tttcgtaatc ttattagctt actgaactat
    17881 tctatcatta attatagtag ttttctacta aattaagcga ctttttaact ctctaataca
    17941 gcttttactt catctttcga tgataacttt aataattcta aaaatctctc ttcatctata
    18001 actttaattc ctaaatcatt tgctttagta agtttagaac cagcttcttc acctgccaaa
    18061 acaaatgttg tcgatttact aacactagat gtagcttttc cacctctttt ttctatcata
    18121 tctttcgcat catttctttt taatgtagga agtgtaccag tcaatacgat tttcatccct
    18181 tcaaatattt tttctacttt ttcacctgta ttatccaatg attttgtatt tactcctaca
    18241 ttttttaact tgtttataac gttcatattt ttgtcttcct taaaaaactc gacaacgctg
    18301 tttgccatta tacttccaaa ttcctctaat tcttctagtt gctcactagt tgcagaaatt
    18361 acttcatcta aatccttaaa gttatatgca agtatcttag ctgctttaac tcctataaat
    18421 ttaataccta atccatttat aagtctccat aaatcatttt cttttgattt ttcaattgaa
    18481 tttattaaat tgtcagcaga tttttcgccc attctctcta ggtcgactac atcttccttt
    18541 tttatataat ataaatcaga tacatcttga ataatttttt catttaatag cagtgtaatt
    18601 atagattctc ccaatccatc tatattcatt gcatcccttg acacaaagtg tataattccc
    18661 cttcttattt gggctgggca agacatattg atacatttta cagcagcctc tccttctaat
    18721 cttattgttg gctctgaaca aactggacac ttttctggca ttttaaaatc tatttcatct
    18781 ccagtacgtt cctcttttat aacttctaaa acttgaggta ttatgtcccc tgctttttga
    18841 actaatactg tatcatttat ttttatatct ttttctctta tataatcttc attgtgaagt
    18901 gtagctctac ttacagtagt acctgcaagt ctgactggtt caagaattgc tgaaggtgtt
    18961 attgtaccag ttctcccaac ttctactatt atatctacta ttttagtttt cttcttttct
    19021 gctgggaatt tatatgcaat tgcccatcta ggacttttgg ctgtatatcc catttcgtct
    19081 ctttgtttta tattgtttac ttttattacc ataccatcta tttcgtagtc taaattaccc
    19141 ctgttatctg tccagtattc aatatgctca ataacttcat ctatactttt acaaacttta
    19201 taactctgac ttactgaaaa tcctagtttt tctaagtact ctagtgattc actgtgactt
    19261 tctaaatcat attcttctat attttccata ttaaatacaa atatatctaa tggtctcttt
    19321 gctgttagtt ttgggtctaa ttgtctcaat gaaccagctg caagattcct tggattagca
    19381 aataatggct gttcttgttc ttcttgttgt tcatttactt ttctaaaatt atcttttgat
    19441 atatatactt ctcctctaac tattagttct tttttatcat ttatcttaag aggtatgctt
    19501 tttactgtca ttaagttttg agaaatatct tctcctacaa ctccatctcc tctagttgct
    19561 cctttttcaa actctccatt gttataagtt agacctacag aaagaccatc tattttaaat
    19621 tcaacaacat attcaacatt ttctcccaca tattcttgaa ctctcttatc aaaatctctc
    19681 aaatcttgct ctgaaaaagc atttgataaa ctcaacatag gagttttatg tacaatttga
    19741 ttgaatttat ctaatggctt acctcctacc ctatttgttg gagaatcaat tctctttagt
    19801 tctggatgtt cttcttctaa agaaattaac tccttcatta acttatcgta ttcataatct
    19861 gatatttctg ggctatcttg attataatac ctttcattat ggtagtttat tgtctctata
    19921 agtttattaa tttcatcttt tacgctcact ttttgcacct cttcatattt ttaaaattaa
    19981 attattttga aatttcccta taattaatta tctcctattt atatattttt tagtatatca
    20041 cttttttgac tttaaattta ttacaaaaaa aactcttatt agtatcttta atacgactaa
    20101 taagagtttt tatttttatc aaaatttttt atttttaata gcctcttgat atgtctacaa
    20161 cattatctac aggtttattc tccattatat agtgtttcaa attattataa accatattaa
    20221 atgttctctc tttgttttta tctgacaccc aagaattatg aggagttact atgacatttt
    20281 cgcactccca caacttattg tccttgttta aaggttcact ttcaaatacg tctagtgcta
    20341 cccctctaaa tttctctaca cattcttcta aatctttctg attaactgta tttcctctac
    20401 ctacatttat aaatacagaa ccatctttca taagtttaaa catatctttg tttactattc
    20461 catctgtact ttttgtagaa ggcattgcta caactactat atcacattct tcaaatacaa
    20521 tattcatatc ttcagtagca aaacacttat caaagtattt tttactactt ccatcagtat
    20581 tgacacccca tatttctaca ccaaaagctt ttaatcgttt tgctgcttct gaagatatag
    20641 tacctgttcc taaaaatcca actctttgtc catacaattc tgatacagaa aaatccatct
    20701 tccattcttt attttgttgt tgtttaaaaa attttgcact atttttatat atttctaaaa
    20761 tatacaatac aatactttca ccaataggta cactatatcc gcctctatta ttagctatct
    20821 taatatttct cttcaatacc tcttcttttg gaagttggtc tatacctaca ctagttgtct
    20881 gtatatattg taaatttttc atcttagaaa tatctaatct ctcaaatgga ttgtatgtaa
    20941 ctagtacatc tgctgtattt acatcctcat tattttcaat actatcttca gagtaatgta
    21001 tgacatcata gcctaattct cttattttat caaatttttc ttttccataa tttattgtaa
    21061 ataaaacttt catctcaaat ctcctctatc tacccacatt taaaatttta tattctaaaa
    21121 tataatatga tactcaatgt acattagtta aaattactat aagatatctt aaatttactt
    21181 atgttcaatt gtttttccta aagacatagc attacgttct tgaactaaat tttctattaa
    21241 attattcaat gtccaatcaa tagatgtctc tgttaccata gctgtaaaag gtctatttat
    21301 tctcactttt tttagacctt ctaaaaatgc atttacttgt gtgcttggag tgttattaaa
    21361 tattacagac ttactcttaa tcccattttc acattcactt gatatatttc catctaaaat
    21421 gtctcttatt atattctctc cgtttttttc acttaagatt atatggtctc ttattccagc
    21481 aaagttactt acgtttttaa ttaatgataa ttctttttta ttaaagttta caagcataat
    21541 acacttccta ttgtgtattt tatctaggtt attattattt attttttcaa atgacatttt
    21601 atccatcctt tcatttattt atatacttta atttacccca tacatttaaa ataatcaatt
    21661 tttatagtta atttttatat taatacccaa attacattta aatagttagg ctatccaaaa
    21721 ctaatatata aaattagaat aattagatta atatctacaa atcataacct aaaggagaga
    21781 taccatgaat acattactaa acaataaatt tagcattaaa aacttttcaa atgcttgggt
    21841 tatagtactt ggttgtatgt taatacaagc tgttccattt agtattgtag caagtataca
    21901 gcctcaattt ataagttatg ttgtagaagg agaaggcttt agtttagctt ctttttctct
    21961 aatattcact attggtacta ttgcctcagc aattgcatca ccttttatag gtgtattatt
    22021 ttctaaagtc aatattaaga tactatattt attaggttgt ataatttcta gtattgcttt
    22081 tgctactttt tctatatgta ctgaactatg gcaattttac actgtatcat gtattgttca
    22141 aattggcgct acaattatat ctgctatagg aatacctgtt ttaataagtg cttggtttga
    22201 tgaagaaagc aaaggaaaag ctttgggctt tgcttttgct ggtggttcta ttggtagtat
    22261 atttctacaa ccacttgcag ttcgcttatt agcaactcaa ggatacagtc attcttatct
    22321 aatatttgga attatagcat taatagttgg tatacctgta atattattca tactcaggat
    22381 gcctaaagat attaatgaaa aggtaaaatc taaaggtaaa aaagagaaag catctgatac
    22441 aatttctcct tcagtatggg gttatacatt aaaagaagct aaaaaaatta aatatttttg
    22501 gattatgact attggattta tatttgttgg tatatatgtt tcatcattat ccgtacaata
    22561 tccaacttat cttagaaatt atctaaatcc tattctaata ggaacagtag gctccacatt
    22621 tgctttattt gctctcattg gtaatttatt tggaggatta ttgtttgata aattcggtca
    22681 aacaaagcca cttattatat cttttgtatt ggttcttata gcaactttat cattactatt
    22741 ggtagataaa tcttctactt ttgcatttgt atttgccatt ttgaaaggtc tttctgtatt
    22801 tgcttatatg attggtcctg cttatttaac tggctctttc tttggtcaaa aggagtttgg
    22861 tagcatatat ggaatcgtac aattaatttt tgcagtggga tttgcattag gttcaacatt
    22921 atttggattg ttagttgata attttggata cagattttct tggtctatta taattttatc
    22981 agttataatt gcctatactt tacttattat aacttctata ggaatgaaaa aattgaataa
    23041 agaaagatta gataaactaa tgcaataatt gatttttaga tatataaata ttataataaa
    23101 aaagatggag cttttaatca taaataaaac tccatctttt ttatattaca atatagctaa
    23161 ttttctctta tttttaactt tactatctat atcctcatcc ttaactatag taaatgcctt
    23221 agttggacaa acatttacac atgcaggccc ttctggcgat tctatacaaa aatcacactt
    23281 atttgctgtc attctttctc taaagcaaag ctttccttca tctacatctt ttaatgtaac
    23341 tatgtctatt gcacctatag gacatgctaa catacaagtc ttacatccta tacaattttc
    23401 ttcatttatt tttattgtgt tcccaaccct aactatacca ccatttggac atgcctttgc
    23461 acaaggagca tcttcacaat gtctacactg tattggagca cttacttctt ttgtctttat
    23521 gacagaaagt tttggattaa aatttatttc atctggattt tggtaaaaaa tatcttcatc
    23581 tgaatgtgca actacacatc ctatcataca agttctacat cctatgcatt tatcagggtc
    23641 tgctataaca aaatagttca tcacttagcc acttcctttc ctatacaata tgtagtgtgt
    23701 aatagatgat gagatttttc tcctaatggt tctaccagat attcatcata cagtttttta
    23761 atatcaggat tctcatgaga ttttctaatt gctaaatgtt catcatgcac gtatgttgct
    23821 tttgttctat tttcataagc caattcctta tattcttcta gtaaaatttt tggttgacct
    23881 ccaccactta cacacccaac aggacaagtc ataacttcaa taaaatctac atctaatttt
    23941 cctttttcca agtcttcaag cactggtatt acatttttta atccacttac tactccaact
    24001 cttaagtcta agtctcctat tttaatagtt gactttctaa aaccattttc tcctcttact
    24061 tgttttactt ctacatcagg aatactttct ccagttataa gctcatatcc tgttcttata
    24121 gcagcttcca taaccccacc agtaacacca aagatagttc cagctccact ataactacct
    24181 aaaggactat caaatttttc ttctcttaaa tttttaaaat caattcccat gtcttttatt
    24241 aaataagcta gttctctagt agttaatact acatctacat ctctatatcc actagaatcc
    24301 atttcttctc tatcacattc atatttttta catgtacatg gcattataga aacactaaat
    24361 atctttgatg catctacatt atttatctct gcaccataag ttttaaatat tgcaccaccc
    24421 atctgctgag gtgatttaca agaagataaa tgattagtta atttaggata atttttttcc
    24481 atgaatttta cccaagctgg acaacaagat gtaaacattg gaagagttcc accttctgtc
    24541 actcttttaa ttaattcact accttcttcc attatagtta aatctgctgc aaaattagta
    24601 tcgtagactc tatcaaaatt taaagctcta agtgctgctg ccatttttcc agcacctaag
    24661 tatcctaaat aatatccgaa atcttctcct aaagcaactc taactgctgg tgcacattgg
    24721 accatagtga atacatttgg gtcacttaaa gctttcttta ccttatctat atcacataca
    24781 ttgtatgctg caaatatagg ttcatttata gactctggta tctctctttc ttgttttttc
    24841 tcttgtaaaa actcaaatcc ttcatctata actgaggcat aagatttaca agtttgaaca
    24901 cattgaccgc acataacaca tctatctaaa tttatctctt gtggctttcc ctcttcacct
    24961 tcaatagcat ttactggaca gacctttgaa cattctctac atccagtaca taaatcctta
    25021 tctatattta ttatcataaa cgtcacctac ccctgtatcc tttatttatt tgttaacaat
    25081 aaactaagtg cagcttgtct attcttagct tttttgtcat ctattggagt aacaagtttt
    25141 aatgcttcct gaggacatgc acttatacaa gctatttttt cattgtcttt acataagtca
    25201 catttataag ctatcttctt attttcatct aaaattactt gttctacttc tttaccatct
    25261 tcatattgtg gaagtaagtc tatagctcca aaaggacatg ctagcagaca agttttacat
    25321 cctatacact tttcttcatt tacagacata atattgtttt ctttgactat agctttttgt
    25381 ggacatgagt ttaaacaagg agcatcttca caatgtctac actgtattgg catacagaaa
    25441 ttgtcacctt ttactaaata aagtctaggt ataacaggta tatcaactgt tccaactgta
    25501 taacccacat tattatgctg attatgcatt gtaaaacaag ctacttcaca agctctacaa
    25561 cctgtacatt tgtttgggtc cgctattaca aaagagccta ttttattttt catattatcc
    25621 cacctttcaa tttgtatatt tccaaccctc tagttacttt catcatatgt ctacttactt
    25681 tcatcatata tatttatata tttcactatt ttctcagtac ttatatttcg tttacatttt
    25741 ttgcacaaat tgtatgtgtt attatacacc ttatcagtat cgtcaattaa tactccacag
    25801 atttcacact ttgaacattt ttgttcttct tttttagtac acatatactt attctcactt
    25861 tttgataact ctctgtggat ttgcataaat attcatccgt cttcctctta caaatccaat
    25921 aagcgttata tttaattttt tagaaagctc tattgataaa ttagtcggtg ctgattttga
    25981 tactacaata ggaatttgag tcatagctgc ttttaatatc atttccaatg atattcttcc
    26041 acttacagcc acaattttat cttttaatgt tatgtcattt aaaacacaaa atccaatagc
    26101 cttatccatt gcattatgcc ttgctacatc ttcacaagtg actatttttt tatcaaaatt
    26161 aaatacagat acgctatgta ccccaccagt atttttaaat aattctgatg aatttaaatt
    26221 tctatccatt atactgtaaa tcgtatcata atttattttt atattgctct ttacagaagc
    26281 acatgttata tagtctagtg aatttaaaaa tataatttgc tcatcttgat ttttttgttt
    26341 ctcttgaata gttacttttg caaaacgttc actctcattt atctcaaatt ttaataagct
    26401 ttttttgttt tctatgtatc cttttgtttt caagaatcct actataagtt cctctaaatt
    26461 atatggggta cataaaaatg tatttacata tttatcattt aaaataaaac taagtggata
    26521 ttctgcaatt atttcttcat tatctattga tacttcatgt tcatttattt tgcttacttc
    26581 tacattaaaa gaatttaaat atttgtagtc cactagacta taactatttt caaaaacttt
    26641 atccttacta ttttcttgta atttagtttt tgagtatata ttgttcataa aatattagcc
    26701 tttctgcctt ttataaatct taaatctaat aaatcattag atatttgtag ctgtcatttt
    26761 gtctttcaaa ctttgatact cttctcttac acatttttct gctaattctt ggtcttcaag
    26821 tttttcaagt ttaacagcac aatatttata ctctggtgtt ttagatatag ggtctaaatt
    26881 tgctattgta agttcattgc aagctccaac ccaccactga taagtcatat atgttgcacc
    26941 ttccttaact ctatcagtaa ctgttgctct tgttattaca cttcctcttc ttgaactaat
    27001 tctaactaat tcatcatctt caattcctaa tttttcagca tcattagaat ttatttgaac
    27061 acgtcctggt tcatcttcta atgaactaag tgttctacaa ttaccagtca ttgttcttac
    27121 tgaatagtgt ccaacttctc ttacagtaca taaactaaat gggtattcat catcttcaac
    27181 ttccataggg ggtctccatt cagcagcgaa caaattacct ttaccattag gagtagaaaa
    27241 cttttgacct tcatacagat acatagttcc tttatcttcc atactttcac tcttacaagg
    27301 ccattgaacg catccttgag tttctatctt ttcataagta gcacctaaga aacttggaca
    27361 taattgtcta agttcgtccc atatttcctt agtattttta taattcatag gatatcccat
    27421 tgctgtagaa atttcgctta taatttgcca gtctggtttt acatctcctt gtggctctat
    27481 agcttttctc atcaattgga atcctctatc tgcacatgta tatactccct catgttctcc
    27541 ccaagaagta gctggaagta taacatcagc gtgaagtgct gtcttattca taaatatatc
    27601 ttgaacaatg acaaattcca attcatctaa tgcttctcta acttctgatg catctgggtc
    27661 actttgcact gggtcttctc caaatatata ataagcttta agctttttct cttttagtac
    27721 taagtctgga acttgagtta aactataacc attattagga gataatttta ccccccaagt
    27781 tttttcaaac ttttctctta tcttatcatc agttacattt tgatagccag gatatacatt
    27841 aggaagagct cccatatcac aagctccttg aacattattt tgtccacgta caggtcctat
    27901 tccaacgctt tctctaccaa aattacctgt aagtagagca attgatgcaa gtcccttaac
    27961 tacatcaaca gcttgaccaa attgacagac acccattcca taaagtatca tagctttttt
    28021 cccttttgca tattctctca tagcttttct tataagctct tctgggattc ctgtaatctt
    28081 ttcagcatat ttagctgtat atggtttaac aatttcttta tactcatcaa atccttgtgt
    28141 atgattttga acaaattctt tattgtaaag accttcttct attaatacat ttccaaaagc
    28201 atttactaaa accatgtttg ttccaccttt tataggtaga tgtatatcag caattcttgc
    28261 agattcagtt actctagggt cagtaacaat taatttagca ccattttttt tagcttttac
    28321 tattctattt gccactattg gatgtgaatc tgctccatta tatccaaata taaataaaac
    28381 atctgcattt tctatctctg gtatagaatt agacattgca ccactaccta atgagtaagc
    28441 cagaccggct acagatggtg catgtcatac acgagcacaa tggtcaacat tattagttcc
    28501 tattgtagct ctcataaatt tttgcattat atagtttgct tcattaccag gtcctcttgc
    28561 agaacctgtt cccataatag aatcaggtcc atatttttct ttaatttcat ttagtcttga
    28621 agctgtatag tttatagcct catcccattc aacctcttct agtacacctt gttttcttat
    28681 cattggcttt tttagcctag gtgttagaat tttagggtca tttagaaaat cccatccata
    28741 acgacctttt aaacacaaac tttcttgatt tgttctacca ttagccggtt cagctcttaa
    28801 tatcttatta tcttttacta ctagatataa ttggcatcca gctccacagt atggacaaac
    28861 tgttaaaatt tttttctcca tacaattccc cccttagatt tatcccctga taagtaaaat
    28921 ttatcttaaa gataatgagt gtttttataa tatgcaaaat cataaatatt ttgcaatact
    28981 atctattaaa ttcaacaatt ttctcacaat tcctcttaaa tttcaaaaaa aatatgtcat
    29041 aataatcttt atatttcaac catttaatta aaatattcta gttcttatga catatttttt
    29101 aatatactca atatatattc tttaaactta tttagttttc acttaaaaca atatttctgc
    29161 caattctatt tttcattcta tagtgagtaa ttacagtaaa tattccactt ccaatcagaa
    29221 ttaaatagta cataaatcca ccatataaaa atactgcata tcccatcaaa ggttgaggga
    29281 atactgattt gaatatagtg aaaaaggcta attcattagc tcctacattc ccaggtgttg
    29341 gtattggaga tattgccata tataaaaatg cttgtaatgt cagtatataa atatagctat
    29401 aaccttgtaa attaaatgct ttatatatcc aaaatgaaac actaaagtaa gcacttaatt
    29461 gaataaaagt tactattata gttgaaatta ataccttttt attctttgca aaaaaattta
    29521 ttgctttact ataatcatct ataaaacttt ctatggattc aatcttatta tctaaaaatt
    29581 ttaaaaactt aatttttgat aaatgcttta tagtaattct cataaa
//
LOCUS       DAEPOZ010000021        29066 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.021, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000021 DAEPOZ010000000
VERSION     DAEPOZ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29066
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.021"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            27..470
                     /locus_tag="KR440_000774"
     CDS             27..470
                     /locus_tag="KR440_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697837.1"
                     /translation="MKVDKTINFLNGFKKTKPCNKIYKIIIVLILTCILFKSFMYVKS
                     INELEDYINQNKLVDKSKHTLPTTDKTREKFSKIKLNEIKKIYETIGINSITKISIDK
                     NKLEMEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            485..1069
                     /locus_tag="KR440_000775"
     CDS             485..1069
                     /locus_tag="KR440_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697838.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVELNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANVLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            1322..2359
                     /locus_tag="KR440_000776"
     CDS             1322..2359
                     /locus_tag="KR440_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBH0697839.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLSGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            2440..2805
                     /locus_tag="KR440_000777"
     CDS             2440..2805
                     /locus_tag="KR440_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697840.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINVAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            2810..3127
                     /locus_tag="KR440_000778"
     CDS             2810..3127
                     /locus_tag="KR440_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697841.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            3175..4779
                     /locus_tag="KR440_000779"
     CDS             3175..4779
                     /locus_tag="KR440_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697842.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVVLKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYINVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            4903..6531
                     /locus_tag="KR440_000780"
     CDS             4903..6531
                     /locus_tag="KR440_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBH0697843.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIAKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            6796..8145
                     /locus_tag="KR440_000781"
     CDS             6796..8145
                     /locus_tag="KR440_000781"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBH0697844.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSAEKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            8301..9380
                     /locus_tag="KR440_000782"
     CDS             8301..9380
                     /locus_tag="KR440_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBH0697845.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
     gene            9725..10633
                     /locus_tag="KR440_000783"
     CDS             9725..10633
                     /locus_tag="KR440_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBH0697846.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGVNIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
     gene            10638..13007
                     /locus_tag="KR440_000784"
     CDS             10638..13007
                     /locus_tag="KR440_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBH0697847.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKKDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRLTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPKDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            13332..14135
                     /gene="proC"
                     /locus_tag="KR440_000785"
     CDS             13332..14135
                     /gene="proC"
                     /locus_tag="KR440_000785"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBH0697848.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEEFGINTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
     gene            14733..17489
                     /locus_tag="KR440_000786"
     CDS             14733..17489
                     /locus_tag="KR440_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0697849.1"
                     /translation="MRDEILSFVNECVLFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSKFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNTILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEIFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALESKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDTIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINGPMLFIDQISTISALEVGNLLMQGKNIEQIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLPNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
     gene            17621..18100
                     /locus_tag="KR440_000787"
     CDS             17621..18100
                     /locus_tag="KR440_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0697850.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTRDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKEIGDLGIDIELRMLPRDKKKQLSDLIK"
     gene            18116..18538
                     /locus_tag="KR440_000788"
     CDS             18116..18538
                     /locus_tag="KR440_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBH0697851.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLANIEYFCMTKDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSIFL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENRNKIKNVM"
     gene            18578..19369
                     /locus_tag="KR440_000789"
     CDS             18578..19369
                     /locus_tag="KR440_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0697852.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMFDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTTLINAIPKVILDGLGVAGTLLPAVGFALLMDMLISKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAMNTLTEGEIDFE"
     gene            19362..20162
                     /locus_tag="KR440_000790"
     CDS             19362..20162
                     /locus_tag="KR440_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBH0697853.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     KVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFLIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            20210..21265
                     /locus_tag="KR440_000791"
     CDS             20210..21265
                     /locus_tag="KR440_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBH0697854.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRANLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGTISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(21425..23371)
                     /gene="feoB"
                     /locus_tag="KR440_000792"
     CDS             complement(21425..23371)
                     /gene="feoB"
                     /locus_tag="KR440_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBH0697855.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIMEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKSNTH"
     gene            complement(23389..23607)
                     /locus_tag="KR440_000793"
     CDS             complement(23389..23607)
                     /locus_tag="KR440_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBH0697856.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            23813..24322
                     /locus_tag="KR440_000794"
     CDS             23813..24322
                     /locus_tag="KR440_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBH0697857.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            24400..24561
                     /locus_tag="KR440_000795"
     CDS             24400..24561
                     /locus_tag="KR440_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HBH0697858.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            24712..25377
                     /locus_tag="KR440_000796"
     CDS             24712..25377
                     /locus_tag="KR440_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBH0697859.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            25443..27227
                     /gene="pepF"
                     /locus_tag="KR440_000797"
     CDS             25443..27227
                     /gene="pepF"
                     /locus_tag="KR440_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBH0697860.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYTKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEKYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            27479..28321
                     /locus_tag="KR440_000798"
     CDS             27479..28321
                     /locus_tag="KR440_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH0697861.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVMANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     regulatory      28554..28637
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
BASE COUNT        11628 a         3142 c         5155 g         9141 t
ORIGIN      
        1 taaatagtat aggaatgatt atttaagtga aagtggataa aacaataaat tttcttaatg
       61 gatttaaaaa aactaaacca tgtaataaaa tctataaaat aataatagtc ttaattttaa
      121 cgtgtatttt atttaaatca tttatgtatg tgaaatctat aaatgaactt gaagattata
      181 taaatcaaaa taagctagtg gataaatcaa agcacacttt accaacaaca gataaaacta
      241 gagagaaatt ttctaaaata aaacttaatg aaataaaaaa aatatatgaa acaattggaa
      301 taaatagtat tactaaaata tcgatagata aaaataagct agagatggaa ggtgtgtgta
      361 gtgatttaga tgttttaaac aacctaagga aaataaagta cataaaatat tattacatag
      421 acagtattgt aagagatgga gaatattata gatttagaat aaaaagttag ataggtgatt
      481 atttttgaga ttaaataata gagaaaaaaa tctacttaaa atattgttaa tattagttat
      541 tatattggca agtgatatag cagtaagtta tttggaagaa aacctaacta gtttgaagaa
      601 tgaaaagcaa aatataataa ataaaataaa taatcaagat aattatcaaa taaaggataa
      661 taagcatata gatgataaat atatagaaag tgttgaatta aataaaaatg aagaattgaa
      721 aaataatttt aataactcat ttttagataa aaaaacaaat aaagatatag tgttattagt
      781 agaaaatgaa ctgagagata ttgtgaatgt agaatatatt aatcaagaaa gtagtttaga
      841 taatcacaat aaaaaacaag ttaatttaga aataaaagtt aatggagaac ttgcaaatgt
      901 gttgaaactt gaaggtgtaa tgaagaagtt aagactaaat aataaacttg aaaagataga
      961 aattaacaaa ttaaaagtca ataattttga tgttcaaaat attccaaatg aatataaaca
     1021 agaaaaagac aatattgttg aatgtaaaat gacagttaga gtcatataac aaaaaagaat
     1081 agataaagta aaaaactatt atcaattaat aaaattttat agaggaatta gttttacgat
     1141 aattcatttc tacaagaggg attgttgatg attttatcga aatcctaatt aaagttaata
     1201 tattgtacca aaatataagt gaatttaata gaatatttac ttatactata gattgattaa
     1261 taaaaaaatg gtataataac aaattgtact ttaatttaat aataagggag tggaagtcat
     1321 attgttaggg agaaatgagc tatgcccttg tggaagtggc aaaaaataca agaaatgttg
     1381 tttaaataaa gatattgttt cagaaagagc atgcagaaaa actgaacttt cacaaaaaca
     1441 atatacagat ttatattcaa agttatatga ttattcaaga caggataagt ttaaagaaga
     1501 atgtgaaaaa gctaaagaaa tgttttatat aatgcaaaat gatagtgtaa atgaaaaatt
     1561 tgaaagattt tttaatactt atttaataca agaccacata atggaaaata aaaaggttat
     1621 gacagttggt tttcttgaag aaaatggtgc aaacctaagt caatcagaag tatcaatatt
     1681 aaaaagtatg tttgaatcat acgtaagtat ttatgaggta aaagaagttt ctgctggaaa
     1741 aatagtatta aaggactgtt taagtggaaa tgaactttgt acagaagatg taaaacttct
     1801 gagaagtttt aaaatcggaa gttgtatgat agctagaata gttgatattg aaggaactaa
     1861 catattgata gatataacta taagtatatc aaatgaggtt aaagatataa ttttaaagga
     1921 tattatgaat ctattcaatc aatatcaaga tgtatacaaa gatatgaagt cgttcttaat
     1981 ttatcataca catatactat acaagtatat acaacagctt ttagacccaa gtatagctga
     2041 atatttaaag agagaaagag ataaaaacga tacaaaagtt aattcagaaa atgaattagc
     2101 tgaagaagaa tgtaaagttt tagatactct taaagaaaat gtagaaaaag aaaatataga
     2161 tgcatgtata gagttttgga atgaatataa gaaaaatcat gaaaatataa aaggagctga
     2221 aaatggttgg gcagctgctg ttgaatacta tgttaaaaaa gaagcaggac aacctataac
     2281 tcaagttcaa atatcaaaga aatacgatat aagctctagt acactaggaa agagatacaa
     2341 agatttaaaa atatcttaat taattataaa aagctttaag tcatagataa agaatctata
     2401 gtataatata tgtataatgt ttagaaagag gtattaataa tgagtcttag tttaggaaat
     2461 gcaattgctg tatcaacgaa ctttataaaa tctaacccag ctgtacctgt gtacaacgga
     2521 gaagaaatat atgcagttat ttatcatata gaaataggat ataataatgg tgtatcacct
     2581 gataccaaac aatatataat aatagactat aaagagtttg atttatctat ggataaaaaa
     2641 aatgaactaa taaatgttgc taaagagtac tgtaaatcta aaggtattgt agaaaacgaa
     2701 aaccttgaaa taaacctttt agacaatgaa gaagcagaga acttcttaga aaaggtgttt
     2761 aataacatga aggttataag aggtcttata actaaaaaag gataaataaa tggactatat
     2821 aggaatagaa aatataacac cttatgaaaa tacatatgaa tttagtgtat atgaatatga
     2881 tgatgaaatc accttaggta gtgaaaagtt atatgtgtgt gaattaaggg ttgtattgat
     2941 taaagttaat tctctgtatg ttgaaagatt gcataaatca gttgaagcaa tggtcttagt
     3001 aaaaaacttg aaaaaggatt tagataaaac acttgttgta aacaaaataa agaattttgt
     3061 gctagatgag atttgggtag aaaatctagt aaaagagaat atagaagtta tatttgtaga
     3121 aagctaggaa taaccctagc ttttttatat tacaatctaa gagaggaaaa caatatgtta
     3181 agagtatcta atgtaaaact tggaattgat aaagatatat ctttattaaa agatgttgta
     3241 cttaagaagc taaaaataaa agaaaaaaac ttagtcaaat atagtatata taaagaatct
     3301 atagatgcta gaaaaaaagg aaaaatggaa tttgtttatt ctgtagatgt agaagtcaaa
     3361 gatgaaaata aaatacttaa ctcaaaaata aaagatgtaa caaaaataaa ggaaatcaga
     3421 tacataaatg tacctgtggg tgataaaaaa ttaaagaata atcctttggt aattggtagt
     3481 ggaccagctg gtttatttgc aggtctgctt ttagctcaaa tgggttatga accaatcata
     3541 cttgaaagag gtcttgatgt agacagtaga accaaagata taagtgattt ttggacaact
     3601 agaaaattaa aaaataattc aaacgttcaa tttggagaag gtggagcagg aactttttcg
     3661 gatggtaaac ttacaactag aataaaagat atacgatgta gaaaagtatt agaagagtta
     3721 gttcagtttg gagcaccaga tgaaatatta tactcacata agccacatgt tggaacagat
     3781 atattaaaaa atgtagttaa aaatataaga aatgaaataa taagtttagg tggtcaagtt
     3841 agatttaatt caaaagtaac agatatagtt gtatcaaatg attctataca atctgtaata
     3901 ataaatgata ctgaaaaaat agatacagat acaattatac tagctgttgg acatagtgca
     3961 agagatactt atgaaatgtt gtattcaaga ggtataaaaa tagttcaaaa accttttgca
     4021 ataggagcta gagttgaaca tccacaagat ttaataaata aagcacagta caaggatttt
     4081 tataatcatc ctaggttagg agctgcagat tatagactta tagagcatgt ttcaaatgga
     4141 agaacagcat atactttttg tatgtgtcca ggaggtagtg tcatagcatc tgcatcaaat
     4201 gattttgaaa tagttacaaa tggtatgagt gaacatgcaa gagataaaat aaatgcaaat
     4261 agtgcatttt tagtaaatgt aacgccagaa gattttaaga gtgataatcc actagcaggt
     4321 gttcatttcc agcagaagta tgaaaaatta gcttttgaat taggtggaaa aaattacaat
     4381 gcaccagttc agcttgttgg agattttata aacaataaga cttctttgaa tttaggaagt
     4441 gttgagccat catatagacc aggatataca cttacaaatt taagtgaatg tttgcctagc
     4501 ttcgtaacag agactatgag agaagcgttt atatctcttg ataaaaaatt gaatgggttt
     4561 gcaatgtatg atgctgtttt gacgggagta gaaactaggt catcatcacc aataagaata
     4621 gtaagagatg aaaatacttt agaatcagtg agtattaaga atctatatcc atgtggtgaa
     4681 ggcgctggat atgcaggtgg tatagttaca gcagcagttg atggtataaa atgtgcagaa
     4741 aaaataatac aaaaatacac caataataca aaaatttaac ataaacttaa tgatttttta
     4801 atatttaaca tgaatttaac attatcactc ctataatttg ataaaataca ataggttata
     4861 aaatttttag atacagtaat ctctttacaa ggaggaaata atttgaacat tataataagt
     4921 ctaatgggtg gattaggtct gttcttatat ggtatgaacc ttatgggaga aggtcttcaa
     4981 aaatctgctg ggacaaagct aaaaaagata ataaaactac ttactagtaa cctatttatg
     5041 ggagttttgg taggtacagg agttacagca gtaatacaaa gttcaagtgc tactactgta
     5101 atggttgttg gtttcgtaaa tgcaggaata atgactttaa aacaggcaat aggagttata
     5161 atgggtgcta atatagggac tactgttaca gcccaattgg tatctttcga cttaactggt
     5221 atggccccag tagcattagg tgtaggtatt atactttatt tatttggaaa taagcctaga
     5281 ataaaaaata tagccgaaat acttataggt tttggtatat tatttacagg tatggatttt
     5341 atgaagatgg cagtggagcc actaagagac tatcaaggat ttactgacct acttgttaca
     5401 tttggtagat atccactact tggattatta ttgggatttg ggattacagc aataatacaa
     5461 agttcaagtg catctatggg tatgttggtt gcactggcag cagaaggtct agtgccatta
     5521 tcagcagcac tgccaattct ttatggccaa aatattggta catgtgtaac ttcactgtta
     5581 tcaagtattg gtgctaacaa aaatgcacga agagcagcta tgatgcatct aatcttcaat
     5641 gttttaggaa cggtgatatt cttaatattc ttgaataaac cagtagtaag catggttaca
     5701 agttgggatc caagcaatgt tgcaagacaa atagccaata cacatacatt gtttaatata
     5761 atatctgtat tgatactttt accatttaca aatcttatta taaaactagc tataaagctt
     5821 gttcctgata gagcaggtga tatagatgaa gatgaaacta aaactattaa atatatagat
     5881 gatagaatga tagaaactcc ttctatagct cttgcaaata cgataaaaga agcacttcgt
     5941 atgggtgaga aggcgaagga aagcctaaat gcatctatgg aagccttagt agaacattca
     6001 actgaaaaaa tagctaagac atatagacga gagagattaa tcaatgattt acagaaagct
     6061 attttaaatt acttacttaa actttcaaaa gcacctttag atgatgattc aagagaagtt
     6121 gtggatactt tgtttaatac agtcaatgat atagaaagaa ttggtgacca cgctaaaaat
     6181 atagctgaat tatcgcaagt tgcaatagat tcaaatattt ctttctcaga agaaggacaa
     6241 tctgaacttg atgttatgta taatagagtt gtttcagcat atacttatgc attagaatca
     6301 atgagaactg ataatgttga tttagcttgt aaggttataa aaattgaaga acaagttgat
     6361 ataatggaga aatcttgtag agctaatcac atgtatagat tgaacaataa tctatgtagt
     6421 atagaaaatg gagttattta tttagacgta ataagtaatt tagagagaat atctgaccat
     6481 gctgtcaata tagcacaaca agttatagcg aagagattag gaaatgatta aaaataatta
     6541 atttaaaata atataaaatt taaaatagag gataaataaa agagaaactt taaatgatac
     6601 gtttagagtt tctttttttg taaatttatt tgactaaaat ttttaaattt atacatagtt
     6661 atagtattta gaagaatcga tttcctgata ataaatgatg agatatgtat gattttgtgg
     6721 caaactttta aaaaaaacac ctatattttg tgttataatc tctttaaaac gaatatgctg
     6781 aaaagaggga tttaaatggg aaaaataaat tttgattata gtaaagctac agagtttttt
     6841 tgtcaaaacg aaatagatgt catgcaacca tatgtagatg ttgcgcacga catgttacat
     6901 aataaaactg gtttaggaaa tactttttta ggatggatag accttcctaa aaattatgat
     6961 aaagaagagt ttgacagaat aaaaaaatct gctgagaaga taaaatcaga ctcagatgtt
     7021 cttctggtaa taggtatagg aggctcttat ttaggttcga gagctgctat agatatggta
     7081 agtcattctt ttaggaatgg acttaaaaaa gagcaaagaa aggctccaga agtatatttt
     7141 gtagggcata atataagctc aacatatatt atggatttat tagatataat tgaaggaaag
     7201 gatatttctg taaatgtaat atctaaatca ggaactacta cagaaccagc tctggctttt
     7261 agaatattta aagattactt agagaaaaag tatggtaagg aagaagcaag aaagagaatt
     7321 tatgcaacaa cagatgcaag taaaggtgca cttagacagt tagctacaga agaaggatat
     7381 gagacttttg taataccaga tgatgtaggt ggtcgttttt ctgtattgac agcagttggg
     7441 ttacttccta ttgctgctgc tggacttgat attgatgcta tgatgaaagg cgcaaatgat
     7501 gctagagaag catttcaaaa tcctgactta aaaagtaatg atagttatag atatgctgtt
     7561 gctagaacaa ttttacatag aaaaggaaaa gatgtagagt tattagtgaa ctatgaacct
     7621 caactacatt atgtaagtga gtggtggaaa cagctttatg gagaaagtga aggaaaagaa
     7681 aataaaggtt tattccctgc ttctgtagat ttctcaacag atttacattc tatgggtcaa
     7741 tacatacaag atggaaaaag gcttttattt gagacagtac ttaatgttga aaactgtaag
     7801 agaaatataa ctataagttc agaggaagta gacttagatg gtttaaatta tttagcaggt
     7861 aagactgtag attttgtaaa tcataaagct tttgaaggaa ctctattggc tcatacagat
     7921 ggaaaagtac ctaatttagt aataaatata cctcaactag atgagtataa ttttggatat
     7981 ttagtttatt tctttgagaa ggcttgtgga ataagtggat atttattggg agttaatcca
     8041 tttgaccaac caggagtaga agcttacaag aaaaatatgt ttgctttact aggcaaacca
     8101 ggttatgaaa aagaaaagga agagctagaa aaaagactta aataatttta aatagttaag
     8161 gtttaattaa gattaaaata atataatgaa atcatcgaaa aggagtttac tcaaaataga
     8221 tgatttcatt ttttttatga aaaattggga aaaatatatt ttgataattt atattatatt
     8281 aagataggga gtggtatgag atgtcaagaa gaaagaaagg gataagttta gtaatattag
     8341 ttgctataat aagttcaata cttagtagtt ttctaacaat aattttagtt aaggacaatt
     8401 tagtaagcaa atctacagga agttctacgc cgatagttgt aaatgatgat ggtaagagtc
     8461 aaaatattta tcaagcagtt gcagaaaaag ctacgccatc tgttgtaggt attacaacaa
     8521 ctagtgttga tactagtaat atgtttgcaa taccaactga aacacaaggt gtaggaactg
     8581 gaatcatagt tgattcaaat ggatatatac ttactaattc tcatgttata tcagatggtc
     8641 aagctacaag tgtaaatgta ttattcaatg atggttcaac aacttcaggt aaagtagtat
     8701 ggtttgacca acaacttgat ttagctatag ttaaggttga taaaactgga ctaacaccag
     8761 cagaatttgc tgatagtgac aaagtaaaag ttggagatat atcaatagca ataggtaatc
     8821 ctcttggatt agattttcaa aaaacagtta ctcaagggat aataagtgga ttagatagaa
     8881 ctatacaaac agaaaaaact aatatgactg gtttattaca aactgatgca agtataaatg
     8941 caggaaatag tggaggacca ttgttaaatc aaaaaggtca agtaattggt ataaacacag
     9001 caaaagcttc tcaagcagaa ggattaggat ttgcaatacc aatcaataca gctaagagta
     9061 tagttgaaga agtaatcaaa aatggaaaat atgaaaaagt aactttaggc ataaaaggta
     9121 cagatgtatc aaattatgaa gcagcaacag gtacaaaatt aagtactgat aaaggtgttt
     9181 atgttgcaga agtaatatct ggttcatcag cagaaaaagc tggagtaaag gtaggggata
     9241 ttataactaa agtaggagat acagatataa caggtatgaa tgatttaaat aaaaagttgt
     9301 atactttctc aaaaggtgct agtacaaaaa taacagtaaa tagaggcgga aaagcagtaa
     9361 caataaatgt gaatttctaa atatgaccag aaagaaggag actacatggt aaagtgtagt
     9421 ctctattttt atttaatttt tatagtatat acatctaaat taattaaaat gatgtattgt
     9481 ttatagttta ttgctatatt ttcttataaa aataaattta ataaatattt aaatttaatt
     9541 tacaaaaaca atataaatat cataataaaa taaaattttt taatattact aaaaatatat
     9601 agacaaaata ataacaaata tagtattatg taattgtaga aatgcttttc ataggtatat
     9661 tctatgaaga tgaattgcat aaaaatgaaa aaatggaatg gatattattt aggggtgaaa
     9721 aatcatgaat ccattagtta taaacttaca aaaatgtagc atacatgatg gaccagggat
     9781 aagaagtaca gtatttttta aagggtgtcc acttgaatgt gtttggtgtc ataatccaga
     9841 gagtcaaaca tatacaaaac aagttttgta taatgaagaa agatgttcaa aatgtgaagc
     9901 atgtataaat atatgccctc ataaggcaat ttacaaaggt gaaacaaaaa tatgcttaga
     9961 ccaagataag tgtgagtttt gtgaaacttg cttagactat tgtgtaaaca atgcaagaga
    10021 gattgttggt caagagtatt ctgtaagaga cttggttaaa gagatagaga aggatagaat
    10081 tttctatgaa gaatcgggtg gtggagtaac actatcaggt ggagaagtaa tggctcaaga
    10141 tatggacttc atttgtggag taataaacat gtgcaaatca aagggaatcc atgtagcaat
    10201 tgatacatgt ggctatgcaa agtcagaaaa ttatgaaaga gttgctaaat gtgcagattt
    10261 gtttttatat gatattaagc ttattgatga ggataagcac ataaaattta caggaaaatc
    10321 aaatgactta atactgaaaa atgttaagat tttaagtgaa ttaggggtta acataaatat
    10381 aagaatacca ttaattgtag gagtaaatgt agatgatgaa aatttagagg ttaaaaaaat
    10441 gatagagttt ttaaagcctt tgaatataca agcagtaagt cttcttccat atcataatat
    10501 tggtaaacac aagtatgata aaatttataa aaagtatgaa ggagaagaac tacaaagacc
    10561 atcagaagaa aaattagagg aaataaaaag actgtttgaa gcaagtaatt tcaatactaa
    10621 gattggaggg taataaaatg gcaagaggaa cttttgagag aactaaaaaa ttaagagaag
    10681 aaagtattaa tgcagaacca cacatatcta ttgaaagagc agtacttatg acagaagctt
    10741 ataaaaaata tgagggaagt gtagagattc ctgtacttcg tgctttatca tttaagcatt
    10801 atattgagaa tagaactctt agcataaatg atggagaatt aatagtaggt gaaaaaggtg
    10861 attctcctaa tggagcacca acatatccag aaatttgttg tcacacaatg gaagatttag
    10921 aagtaatgca caatagagat ataataaatt ttagcgtatc agaagaagct agaaaaatac
    10981 ataaagaaga aataattcct ttttggaaaa aaagacaaac aagagataag ataataaatg
    11041 caatgacacc tgagtggtta gcagcatatg aagcaggtat gtttacagag tttatggaac
    11101 aaagagcccc aggacataca gtttgtggag atacaatata taaaaaagga tttttagatt
    11161 taaagaaaga catagaagca agattaaaag agctagattt tcttaatgat ttagatgcat
    11221 ataataaaaa agcagattta gaagctatgg caatagcttg tgatgcaatg gttatattag
    11281 gtaaaagata tgctgagaaa gcaagacaaa tggcagaaga agaaacagat gaagctaaga
    11341 aaaaagattt attactaatt gctgaaactt gtgatgttgt accagctcac aaaccagaaa
    11401 cttatcatca agctatacaa atgtattggt ttgttcatat aggggttact actgaactta
    11461 atatatggga tgcttttact ccaggaagac ttgaccaaca tctaaatcct ttctatgaaa
    11521 gagatgtaga aaatggaata ctagatagag atagagcaca agaattactt gaatgtttat
    11581 gggttaaatt taacaatcag ccagcaccac caaaagttgg tataacatta aaagaaagta
    11641 gcacatatac agattttgca aatataaaca ctggtggaat aaatccagat ggtcaagatg
    11701 gtgttaatga agttagttat ataatacttg atgttatgga tgaaatgaaa ttgatacaac
    11761 ctagttcaaa tgttcaaata agtaaaaaga ctcctcaaaa attcttaaag agagcttgtg
    11821 aaatatcaag aaaaggatgg ggtcaaccag cattttataa tactgaagca atagttcaag
    11881 agcttatgga agctggaaaa actatagaag atgcaagact tggtggaacc agtggttgtg
    11941 ttgaaactgg atgttttggt aaagaagcat atgttctaac tggatatatg aacattccaa
    12001 aaatacttga attaacatta aataatggat atgacccaat tagtaaaaaa caaataggta
    12061 ttgaaactgg agaccctaga aatttccaat cttatgagga actatttgaa gcatttaaaa
    12121 agcaattaca ttatatgatt gacataaaaa tagaaggaaa tgctgtgata gaaaatatat
    12181 gtgcaaaaca catgccttgt ccacttatgt ctacaatagt tgatgattgt atagaaaaag
    12241 gcaaggatta ccaaagaggt ggagcaagat acaacactag atatatacaa ggtgttggta
    12301 taggaactat aacagatagt ttaacagcaa taaaatataa tgtatttgat aagaaaaaat
    12361 tcgatatgga tacattgctt aaagcacttg atgctaattt tgaaggatat gaagctatac
    12421 ttaatttagt ttcaaataag actcctaaat atggaaatga tgatgattat gctgatgaaa
    12481 taatgcaaga gatattcaat gcatattaca atgaggtaac tggtagacta actgtttgtg
    12541 gtggagaata cagagtagat atgttaccaa caacttgtca tatatacttt ggagaaatta
    12601 tgggagcaag tccaaatggt agactttgtg caaaaccagt ttcagaggga atttcacctg
    12661 aaaaaggtgg agatactaat ggacctacag cagttataaa atcttgtgct aagatggacc
    12721 acataaaaac tggtggaaca ttattaaatc aaagatttgc accatcagta gttcaaggag
    12781 aaaaagggtt agataatatg gctaatctag taagagcata ctttaatatg gatggtcatc
    12841 atatacaatt taatgtattt gataaaaatg tactgttaga ggctcaaaag aatcctaagg
    12901 attataaaga cttaatagtt agagttgcag gatatagtga ccatttcaat aatttaagta
    12961 gaacactaca agatgagata ataggaagaa ctgagcaaac attctaggtt tgcttgtaaa
    13021 taaaacttct tactatataa aatcgtaact gatataataa agtataaatg gtttgattcc
    13081 atttttaaaa ttttagatta attatgagct aactttctaa ataggaagtt agctcgttaa
    13141 gtttaatgaa aaaatcgtta aatttatatt tagtaaatca tacacatatt agtaagtaat
    13201 acatacaaaa gaataattta ttatagttat aataaggtta tattttataa aaataagata
    13261 attttaagca gttaaaataa agtcaattta gctactttaa aataaaacta atagaaaaaa
    13321 ggagatttga tatgaaaact ttaggattta ttggttcagg gaatatggga agtgcaatga
    13381 taggtggcat agtacagtca ggattagtaa aggcagaaga tataactgta tcagatttaa
    13441 gccaagttgc cttagataaa gtaaaagaag aatttggaat aaatactaca actgattcaa
    13501 aggtagctgt tacaaattct gatgtggtaa tagtagcact taaacctaat atatgcgaaa
    13561 aggctttaac tccacttaaa gaattaatag atgcaaataa aataatagta tctatagcag
    13621 caggtaaaac tatagaaagt ctggaaagtt ttataggttc agataaaaaa atagtaagag
    13681 taatgcctaa tactccagcc cttgttggcg agggaatgtc agctctttgt aaaaatagta
    13741 atgtaacaga tgaagagtta aatatgatta aggctctgtt tgaatctttt ggtgaggcag
    13801 aaatagtatc agaatattta atggatacag ttacaggtat tagtggttct tctcctgcat
    13861 atgtatttat gtttatagaa gctatggcag atgcagcagt attagctgga atgccaagac
    13921 aacaagctta taaatttgca tctcaagcag taatggggtc agctaagatg gttcttgaaa
    13981 ctggaaaaca tccaggtgaa ttaaaagata tggtttgttc tccagcagga actactatag
    14041 aagctgttaa ggtattagaa gaagaaggct ttagagcagc agttataaaa tctattgttg
    14101 cttgtataga taagtctaaa gatatgagca aataacagat gtaattaagt ttactatatg
    14161 gtttcttgaa ttgttttttt agtacttatt tatatctata cataaatgta tttggtataa
    14221 gtaaaataag agtaatttac tataaaaagg aatttaatga ttgagtgaga atttattact
    14281 taaatattaa taattaaata gatatatttt taactatatt tgagatatat cattgtgaaa
    14341 aaataaaatt tataagtatt attgtgaggt tataagtaga tttatttaaa aataaattta
    14401 tttatagcct ttttaaattt gaaataatat tcataaggta ataaaagtta caaatatttt
    14461 cttcgctaat aagttttaaa atacctataa tgttttactt tttattataa taaatacaat
    14521 aaatatattc ttatataaat tctatgtcag atatatggaa agataagaac cttgagatta
    14581 tgttaaaaaa tctagatttt atacttataa aaagtttgat tttacaacct tacaaaagag
    14641 aatcaagata ggaatataat tatctttttc ataaatatat aaatgatata ctaatattat
    14701 agtattattg atatttgtat tggaggttga acatgaggga tgaaatttta agttttgtaa
    14761 atgaatgtgt attatttaac aactttgatg aacttaaaaa agaaagtggc ataataacag
    14821 aagatatagt aaaaaagttt aatttaagta gaactcaatc aagtagatta ttaaatgatt
    14881 tagtaaaatc tgataatctt ataaagataa acagtagacc tgttattttt ttacctaaaa
    14941 ataaaataaa tgatgtagta ggacctacaa aaaaaagtat ttatgaaaat ttagatgaac
    15001 ttaaaaatga gtgtaaatta ttaaagcaag agactgtgtt taatagtatc ataggatatg
    15061 ataatagctt aaaagaagta atcgaacaaa taaaaaccgc tgtactttat ccaaatggcg
    15121 gacttacaat aatgctgaca ggtgaaagtg gagtaggaaa aactttttta gccaagataa
    15181 tatacaaata tagtgtaagc agtaatgtat tagaaaaaaa ttctccatac aatgtgttaa
    15241 actgtgcgca gtattataat aatttggagt tattgtctag tctattattt gggcatgtaa
    15301 aaggagcata tactggagca aatgaatcta gagcaggtct aatagaacaa tcagatggtg
    15361 gtgttttatt tttagatgaa gtacatagat taaatagtga aggtcaggaa aagttattta
    15421 catttatgga tacaggtacg ttttctagga ttggagagaa tggagtaatt agaaaagcta
    15481 gggtaagatt aatatttgca acaacagaaa gtctatccaa atttttgcaa acttttttaa
    15541 gaagaatacc cataaatatt tatgttccca atatagaaga aagagggact attgaaaaaa
    15601 gaaagattgt agaacatttt atttatgaag aaagtaaggt acttggcttg cctatagaga
    15661 taactcaaat tacattaaat acgattttaa agataaaatt caaggggaat ataggtgaat
    15721 gtaaaaatgt aataaaatat gcatgtggac gagcttatgc aaaaaatcaa agaagaaatg
    15781 gaaatatttt tgttactctt caagacctcc ctagaaatct ttatatagaa aatgtagata
    15841 tatttcaatt ccaaagtggt aaaagcaaaa gcattatctt ttctcctaaa caatttagta
    15901 ttgaaatgtc aaatggaaga aataaaagaa atataatcta tcaaagtttc ataaaatgtt
    15961 tagactactt tgatagacta gaaaaaaatg aaataacaaa agaaatattt agtaaaaaga
    16021 gttctaatct aatgattaga ctaatggaca atttagtctt ttcaaataat gatgaaaata
    16081 ataacgtact tatggagctt ataatatcga taatgcagga tatatttaga aatttagaag
    16141 ttaataacaa tataaaatac gatgggaata atgtattggc attttcgtct tatctttatt
    16201 tgaaggacga atcttatcaa ttatctgatg agtataaagc tttagaaagt aaattactaa
    16261 tatatttaaa aaaagagagt tacaaagaat actctataat aagtaaaata tcaacattaa
    16321 taacgaatag attggatatg tatcttaata atgtagatac aatcataatg attatattta
    16381 ttaaatcaac aattataaga atgaaattta atcaagttaa tgcgactatt atagcacatg
    16441 gatatgctac tgccagtagt atggcgaatg tatgtaatcg gatgttgggt aaaaatatat
    16501 ttacagctat tgatatgcct atagatgcta ccattgctga tgtatcaaaa caaatgtcta
    16561 gatacctaga aggagatagg ataaagaatg gtttaattgt gttatttgat atgggctctc
    16621 ttaatcttat ctatgaagat ttaaaaaata agataaatgg gccaatgtta tttatagacc
    16681 aaatatccac tatttctgca ctagaagtag gaaatttatt aatgcaaggt aaaaacattg
    16741 aacagattgc agaaattatg gatgaagaaa ctaaacctaa tatacaattg tattatcctc
    16801 aaaaaaataa agaatatgct ataatatcat cttgttttac agggataggt actgctataa
    16861 aaatccaaca tctactttcc gaaagtttaa ttgggatagt tgatgtcaaa ataatacctt
    16921 atgattatga tagtcttgtt tctatgggag ataaagaacc agtttttgaa atgtatgatg
    16981 tgatggctat agtaggaact gctaatccaa atgtgaatgg ggtggatttt atattgttag
    17041 aggatataat atcaggaaaa ggagaagatg atgtttttag aatatttagt agagtagtag
    17101 aaaatgaaaa aataaagaac ataaatgatt ctattgtaaa gaatttttca ttaattagag
    17161 taatagacag tctaactatt ttagattcaa aaaagataat agagagaata gaagaaggaa
    17221 tcaatgctat agaaaatatt caaaagaaaa aacttccaaa tgataaaaag atttctttat
    17281 atgttcattt aagttgtatg attgaaagat tagttagaca aactgaaatt gaagagtata
    17341 ctgatatgga tttgttaatc aaaaaccatc atagtgaagt aaaactaata aaaaatgcat
    17401 ttagtgtgct agaaaagagt tatagtgtgc aaataccaat ttcagagata ggatatattt
    17461 ataatattat ctatggtcta ccacagtaaa atacaagatt acaaaataag taaatgaaat
    17521 ttaaaatagt atgccagtat ttggcatact ttttgctttt atagataaat gagagtataa
    17581 acaataaaaa ttttaatgta tgatggagga tttggggaaa atggtatctt tgatgagaat
    17641 tgatgacaga ttgatacatg gtcaggttgc atatggatgg gcaacatcac taggtataaa
    17701 tgtaatacta gttgcaaatg atgaagcaaa aaatgaccaa atgaaagcta tgtcactgaa
    17761 tttagcgaaa cctagcaatg taactttata tataaggggg gtagaggaat ctggagaaat
    17821 agtagaaaaa tttgcttctt caaagaaaag caatgtatta gtgttagtaa aaaataccag
    17881 agatgcttta gttgtagcta aaaattcagg aggagtatta aaagaaataa atgtaggagg
    17941 gttaagatat gaagaaggaa aaaagaaact tacagattta gttgctgttg atgatgatga
    18001 tataaataac tttaaagaaa taggtgattt aggaattgat atagaactta gaatgttacc
    18061 tagagacaaa aagaaacaac tatcagattt aataaaataa tttaggagaa agagaatgaa
    18121 aaaatttcta attgcaacac atggagaatt gtctagagaa cttgttgaca caagtaaatt
    18181 aataattggt tcattagcta atattgagta tttttgtatg acaaaagata aatcagcaga
    18241 tgatgctgag aaagaaatta gaagtatatt atcaaataat aaagatagtg aagaatttgt
    18301 tgttttaaca gatgtgtttg gtggaagtgt ggcaaatata tgtacaaact tacttttgca
    18361 agggttcaaa tttgaattgt tgacaggcgt aaatttacca atgcttttat ctatattctt
    18421 attagttgag gaggatacaa aactaatagt taaatcaggc ttagaggaag caaaaaatgg
    18481 aattgtttat gtaaatgagt tattagaaaa tcgaaataaa atcaaaaatg taatgtaatt
    18541 taatgtaaat taaactaagt taaaatggag gttcaatatg ttagtacaag ctactatgtt
    18601 agctattata gcaggagttg gtatactaga tgggcgtata tttggtcaat ctatgtttga
    18661 taggccaata gttacaggta tgttagttgg attagtttta ggagacataa aatcaggaat
    18721 tatgattggt gcacagcttg aattaatttg gatgggaatt gcagggatag gtgcagctac
    18781 tcctccagac gtagtaacag gaggtgtatt gggtacagct tttgctatat tatcaggtaa
    18841 tggtgcagaa gttgctttag cagtggcagt gccagtagca gtattagcac aatctcttgg
    18901 agtattagtt agaataataa attcgtactt ttctcaaaag gcagttttct atgctaaaca
    18961 ggctgatttt aagaaagtta ccataatgat gtggttgcca gtaatattat tctttttaag
    19021 tacatcagtt ccaacctttt tagcaattat gattggtgct gataaagtta caacattgat
    19081 aaatgcaata cctaaagtta tattagacgg attgggtgtt gcaggaacat tgttgccagc
    19141 agtaggtttt gcgttgctta tggatatgtt gatttcaaaa aagatgtctg tatttttctt
    19201 tatagggttt ttgttagcat cttatggtgg attagatata actgctatag ctttatttgg
    19261 agtatgtgta gcatttgtac taaactttta tatcaataac aaagaaaata atcaaaatcc
    19321 tcaattagca atgaatactt taacggaagg agagattgac tttgaataaa ttaacaaaaa
    19381 aagaattaag aagtatgttt tggcgttcat ttgctcttca aggtgcattt aattacgaaa
    19441 gaatgcaaaa cttaggttat tgttattcaa tgttacctgc aataaagaag ctatataata
    19501 agaaagaaaa tcaagcaaaa gcaatagaaa ggcatttaga gatttttaat acaacgacag
    19561 tggtagttcc tgctatatta ggtataacag cagctatgga agaagaaaat gctaataatc
    19621 cagaatttga tgaaagttca attagtgctg ttaaaacagc tcttatggga cctcttgcag
    19681 gaattggtga ttcattattc tggggaacat ttaggattat agcagcagga gtaggagtat
    19741 cacttgcaaa agaaggcaat atctttggac cactattatt cttattatta tacaatatac
    19801 cagcatttgc ccttagaata tttggactta agtatggtta taaagtaggg gtaaattctc
    19861 ttgaaagaat acaaagagag ggacttatgg agaagataat gtctatggct acaactgtcg
    19921 gtttattcgt tgtaggagga atggttgcaa ctatgttgag tataactact cctttgaaat
    19981 ttaacttaaa tggtgcagaa gtaattttac aagatatact agataaaata atacctaata
    20041 tgttaccttt aacctttgca tttttaatat attacatgtt gaagagaaag gtaagtgtaa
    20101 caaaattaac tataggcact atagtaacag gaatcgcttt acatgctata ggtttgttat
    20161 agaaattaat tataatttat tgaatatata gataaggaag gtgtataaaa tgacaataca
    20221 agattatatg ttagaaacac cagttagaat gagagaaatt atatctaatg cagatagctt
    20281 atttaatgaa gttaagagag caaatctaaa aaagattata ataacaggtt caggtactag
    20341 ctatcactca ggagtgcaag tgcaaccata tttacaaaat ttattagata tagatgttgt
    20401 gaaaatgtat ccatttatga taactgaaga tacattcaag tttgataatg aaaataccct
    20461 agtagtaggt gtatctcaag gtgggagtag ttattctaca tacaacgcta tgaagttagc
    20521 tgaagataag ggttgtaaaa tagcatctat ggcaggttgt aagaatgctt taatagatga
    20581 gatttctgat tatatattaa cagtaaattg tggtgaagaa aaatctggag ctaaaacaaa
    20641 aggatattat tgtacaaagt taaacttaat gttattaggt cttcaaatag ctagagaaaa
    20701 aggtacaata tctagtgaaa agtacaatga ggaaataaat aagatattag atgcaattaa
    20761 tagatttgaa gcagtgtata agctctctaa acaatggatt gaaagaaaca aagaaaagtt
    20821 agttaattct aaagaaataa gaataatagg tcactctgat atatatggag atacattaga
    20881 agcagcatta aaattattgg aaacaatgag aatacctgta actgggtatg agtttgaaga
    20941 gtttatacac ggtatatata atgctataaa tagtgattct actatattta tactagatac
    21001 aggaaaagaa cctagagtaa ctaagatgat tgatgtactt tctggttgga cggaaaatgt
    21061 atttgctata ggtagagatg ttacagaaaa tgataaaaat ttaaagattg atatcacaga
    21121 taatccttac tatcaaacat ttaattttat agtacctata caactaatat gtggggaaat
    21181 acctacatta agaggcgtag acccaagtgt tccaaaagat actaggttcc acatgaagtt
    21241 aggtagcaag aaactgaata agtaacaaaa aaagaggttg tctcaaaata gagataagcc
    21301 tctttttgac tgttgaaatg atgaaaatat atttgatttt atataaaaaa gagtgcctca
    21361 tgaactaaaa agttaatttt gagacaccca cttttatctt attttaaact aaaatattta
    21421 tatattagtg tgtattactt tttttattgc tgtcttcctt acttgagcaa gaattagcac
    21481 ttggacatcc agagcaaata gaacatccac tacagacatt tccgtttttt gtagacttga
    21541 atttattaaa cagaataaag agtgcaagga ctataattat tccacctatc aaagcttcca
    21601 ttattgaatt acccataaat agtactccac ctatgctttt aactataaaa gccattatcc
    21661 atgctagtat aaattgatat gcaaatgatg taaacatcat tttattgcca tattcttttt
    21721 tcatagttgc aagtgcagct atacatggag tatataaagc agtaaatatc aagaatgtat
    21781 atgcagtaac tccattaaat attgttggta agacaacagt tagattacca tatagtatat
    21841 ttaaagagtt tactacgatt tcttttgctc caagaccact taaaatagca actgaagttc
    21901 tccaatcatc aaaaccaaga ggtttgaata taggagatat aatatgacct agagtagcca
    21961 agaagcttac atttatatct tctgtatatc caaaaacatt gaatgaagaa agtccccaaa
    22021 ttacgactga cattgcaaac atgacagtaa caactcttat caagaaacct tttgatttat
    22081 tccaagtatt tttaataagt gcatctatag tagggattct atattctggt aattctaata
    22141 taaatggttc agcctctgtt ttaaaggctg ttttatttaa gaataatgca actacaattg
    22201 ccataactat acccaccaaa tacagtgacg tagttactaa agcagcatta tgtggaaaga
    22261 atatagctac aaatagtgca tatataggta gctttgctcc acaagtcata agtggtgcaa
    22321 taagagctgt aactcttcta tctttttcgc tttccaatgt tctagttgcc attatagctg
    22381 gagaagaaca tcccataccc ataactatag gtataaaagc tttaccagat agaccaacct
    22441 ttctcataac tttatccatt aaaaatgctg ttcttgacat gtatccacta tcttcaaaca
    22501 acgatatacc aaagaataat gtaaatatta aagggaagaa tggtaaagta ccaccaacac
    22561 ctccaattat tccatcaact ataagtgatt taaaccatgg gctagagtta gataacatat
    22621 cactaacagg tgcagaaata taagcctcta taagactagc aaatccctct tgtagtggtc
    22681 ctccgaccca gtcaaaggta aatttaaata agaggaacaa tacaccaata aatatcggat
    22741 atgctaatat aggattcaag actatattgt ctatcttatc actgatagat ttcttttgtg
    22801 tatatgtagt ttttgtacat ctcgataaaa tggattctaa ttttttatat gtttctgttt
    22861 cacttccaaa gtttttctga tatataaaag ctttttgcgt agctccctta atctcttctt
    22921 ctatcctatc aacaccattt ttcttttttg ctataattgg aacaacaatt actccaagtt
    22981 cttcacttaa ttttttggca tctatattaa ctcctttaga ttctgcaatg tctaacatat
    23041 taagcaatat aactataggc ttgttaaatt gcataagttg agttgttaag taaaggtttc
    23101 ttgataaatt agaagcatca actacattta ctattacatc aacatcttca ttttctaaat
    23161 atgatttaga aaccttttct tcatttgaga aagtatccat tgcatatata ccaggtaaat
    23221 cgacaacctt tatctctttt ccaagaaatc cttctttttt ttctatagta acaccaggcc
    23281 agttacctac atattgattt gaacctgtaa gtagattaaa aacagtagtt ttaccgacat
    23341 ttgggtttcc taataatgct acattaatca cgggtagctc ctcctctatt attgtaaaga
    23401 aatattttta gcatcagatt ttctaatcgc aaggtcaaag cctctaaatc taattataat
    23461 tgggtctcca agtggggcaa cctttttaac ttctatttca gtactattta tacatccaag
    23521 tgcaagcaat cttttagata atttaatatc tccatctata ctattaataa atcctttttc
    23581 tcctattttt aaatcgtaaa cagtcattat taacacctcc taatgaaatt cattatcatt
    23641 aaaattgtaa caagtatgat acaaaaagtc aagatttata gaggtaattg cacattgaaa
    23701 taactaatat ttattctaaa tagattaaaa atattttagt ctaaattagg tgtttttata
    23761 tattttgtat tataatgtaa gatgatagat aaaatttgga ggagttaact taatgaaaac
    23821 tagaaaaatg acatttttag gtcttatgat agggtatagt ttagctttat atgtactaga
    23881 gacatatata ccaaatcctc ttatagttat atttccaggg gcaaaattgg gtttgagcaa
    23941 tattataact ttaatttcat taatgttgtt aggtgtcaaa gatactgtta taattgttac
    24001 tatcagggta attttgtcat caatatttgc aggcccttta tcatatctac tatttagtat
    24061 aggaggagct tatctgagtc ttttatttat gtatttagcg agtaaaataa aagatttatc
    24121 actaataggg ataagcatta taggtgcaat agggcataat ataggtcaat taatagttgc
    24181 aagtttaatc atagaaaata tacttgctat agggtactta ccatttatgt taatggcatc
    24241 attagtaact ggtatgtttg taggcttagt atccaggtat acaataccta aattaaagca
    24301 tttttatagt aagttttatt gacataatat tgacaaaata taatattgtt tgataaaatg
    24361 tatataaaaa tatacaaatt tgtaatagtg gtgattatta tggcgaacaa tgaattagaa
    24421 gcgttaaaaa atgagattga agcagttagg gaagagataa atacatacat agaatatcca
    24481 gaaatatttc aagaagagtt agtagaatca agtaagaaaa tagatatatt aataaataaa
    24541 tacatgtacc taagcaaata gcccacagtt gtgggttttt ttgtatcaaa aattaacaga
    24601 tggctatatt ttacatatat taataataat attaagatat aaaatagtat ttctgatgtg
    24661 taaattatct gtaggacata agttgtaatt aagtcaggag ggttttatat tatgattgat
    24721 ataggaagta aggagatagc aataaggtta ctattagcac taataatagg aggaataata
    24781 ggcttagaga gagaaaaaat aagacagttt gcaggattta ggacacatat actaattgca
    24841 ataggttctt gtattgtttc aataacttcg attcaattat tctttgatta taatttgtat
    24901 acaaatgctg acccagcaag aatgcctgct caagtattat ctggaattgg ttttttaggt
    24961 gcaggagcaa ttttaaaaaa taaaggtgga gtaaaagggc ttactacagc tgctgggatg
    25021 tgggcaactg catgtattgg tatagcagtt ggatatggtt attatgaatt ggcaattcta
    25081 ggtggattat ttgtaatgat agcattattt gtgcttagga taatcaatac gtttttattt
    25141 agccaagaaa agaatgtatt gatattaaaa ttaagcaatt tggactctat acctttttta
    25201 tatgataaat ttgaaaaaaa tcaacttatc gtaagaaata tggaaataga atgtcgtaat
    25261 gaagaatact ggaaggttaa tttttttgta tcttatgcta agagaataca cttacacgac
    25321 gttatgaaag aaataaatat gatagaagga gtaataaatg tagattattt ggactaaaat
    25381 atatacaata tattttgtaa agtgaataaa atataaaaca aattaaaata gaggtgtatg
    25441 atatgggaac tgatagaagt acaatagaag aaaagtacaa atggaagata gataagatgt
    25501 actccagtaa agaggaaatt gaaaaagaca taagtaaagt aaaaaatctt atccaagaag
    25561 taaaagaata taaagggaag ctttctgaga gtaaggagaa cttatataaa gtattaaata
    25621 tctctgaaaa tgcatctagg attgttcaaa atttatatgt atatactcat atgaaacaac
    25681 atgaagatac tagaattaat gataatcaag gaagagccac taagacagaa atgttatcta
    25741 ctgatttagg tgtggctact tcttatatag ttccagaaat actggcgata gaagagaata
    25801 agttaagtga atacttaaaa gatgagaagc tatcctttta tacaaaatat ataaaggata
    25861 tattgagaga taaaccacat actttgagtg aacgagagga agaaatactt gcagctacat
    25921 cagaattatc gacaattcca gaaaatgtat acgatatgtt agcatatgca gatatggaat
    25981 ttcctgagat agaagatgag gagggtaata aggttaagct ttctcattca aactactctt
    26041 tatttataaa aagtaagaat agtagagtta gaaaagacgc ttttgaaggt gaattttcaa
    26101 catatgagaa atataaaaat acatttgcct caacgcttta tggtggaata aaatcagaga
    26161 tattttacgc aaaaactaga aagtataatt cagcaataga agcgtcttta ttttcagatg
    26221 atgtaagttt agatgtttac aataatctta tatcagctat agataaaagt attcctaatc
    26281 taaataaata tgttgagctt aaaaagaaat ttttaggatt gaatgaaata cacatgtatg
    26341 atttgtatgt tcctttgaca gataaatttg acatggatat accatatgat aaagcacagg
    26401 atatcatatt agaggcactt aaacctttag gagaaaaata cttgaaggtc ataaaaagtg
    26461 cttttgatga agggtggatt gatgtatatg ataatgaagg taaaaaagga ggagcttatt
    26521 cttggggaag ttatgattca catccttata tattaatgag ctataaaaat gaccttaatt
    26581 ctctattcac tttaattcat gaattagggc actctgttca cagtcattat tcaagaacaa
    26641 gtcaaccatt tttatattcg gactataaga tattcgttgc agaagttgca tctacattaa
    26701 atgaattact tcttataaat tatttattag aaaattctaa gtctaaggat gaaactatat
    26761 acttattaaa ttattattta gaacaattta gaactacagt tcatagacaa actatgtttg
    26821 ctgaatttga aaaaattgta catcaaaggg tagagagtgg agaaccactt actgctgatg
    26881 aatttactaa tatatactac aaattaaatg aaaagtatta cggaaaatct gcaatagtag
    26941 ataaacaaat aggaatagaa tgggcgagaa tacctcattt ttattcaaac ttctatgtat
    27001 ataagtatgc cacagggttt tctgctgcaa gtgctttaag tcaacagata ctttcagaag
    27061 gaagtactgc agtagataga tatataaact tcttaaaaag tggagggtca gaatacccac
    27121 tagaacaatt aaaatctgct ggtgtagata tgactaaaaa agaatctata gaggaagcct
    27181 taaatgtatt tgctgaatta gttaataaac tagaaaaaga attataggat gtgataggtt
    27241 gatagcttca atgaagactt ctgataatat gagttgtatt gtgtttatat ctgcctaaag
    27301 taactttaaa aagttaatct aattaagaat aaatcatata aaattaagtt tttacatgta
    27361 tttaacaata tttacattta cttaaccctg ctctaatatt catttaacaa agaatctgta
    27421 ttattaaaat atagaaaaat gacaacaaaa aattaaaata taagcggggg taagtaaaat
    27481 gtttaaaaag aaaataggcg ttcttttagc tagtacaatg ttagttggag ctttagcagt
    27541 aggatgtagt tctaactcag gaggttcagg tgattcagga gattcaagca aagtttctgt
    27601 ttcaggttca acttcaatag gtcctcttat ggaaactatc ggagaaaagt ttcaagaaaa
    27661 aaatcaaggt gttagtgtag aagtacaaca gattggttct tccggaggta taaaaaatgc
    27721 catagaagga acatctgaaa taggaatgag ttcaagagat ttaaaagatg aagaaaaaag
    27781 tgctggatta aaggaaacta aaatagcaat agatggtata gcattgataa ctaatactgc
    27841 aaactcagta aaaaacctga caatggacca agttaaaggt atatatacag gaaagattac
    27901 taactggaaa gaagtaggag gaaatgatgc accaatagta atagtatcaa gagaagaagg
    27961 ttcaggaact agagatggat tccaagaaat tgtagggttt gattcagaag aactaactaa
    28021 agatgcacaa ataagtgatg gttcaggaaa tataaaaact acagttgaag gaaatgaaaa
    28081 tgctatagga tatatatcat ttggatatgt tgatgagaaa gtaaatagtt taaaaataaa
    28141 tgatgttgaa gcaactgatg caaatgtaat ggctaataaa tatccaatat caagaccatt
    28201 tatattagtt aataaagaag atggtttgtc agaaaaaggg aaagctttaa tagattatat
    28261 attaagtgat gaaggacaag cacttgtttc agaaaaagga tttattaaag ttaatcaata
    28321 acatgaatta taatataaag taaacatata ataccctgtg agataagctc ataaaaatga
    28381 tgctttaact tgcagggtat tgtctattat tgaatgtaga aatataaata tctttagata
    28441 aaataggtat tttagtatct tatatctaaa aatatggaaa tattgacaaa ataatattac
    28501 aatgttagaa taaaaaagaa atttatgaaa atatataaaa gatttttaat aaaaatattt
    28561 tagaaactga gaagtatatc ttattattgg gcatctggag atatatggag ttagtggtgc
    28621 aaccggctat gaatataatt tttatattgc atagtctttt tttgctttaa aattaacaaa
    28681 aatgttgcaa aatttgaaaa taagagtatt agtcgttaag atttttattg ataggtgaaa
    28741 ttttggcttt taaagtagcc atttgttgga aaagggaaat tttttaaaaa gtataatact
    28801 attataataa gaaaataaag tttttataaa cattgagatg caaaatataa accttttgta
    28861 acaatatatt taccaaaata atacaattta ttttatatta taaccatata aatcatataa
    28921 atcaatactc aaactagcca atagacaagt ttctagaaaa ataataaatt tataagtgaa
    28981 aatgacttta agctccattc tccaacaatc ggagacttta attagtattt aataataaat
    29041 cttaatgttc aatacaatat tcaaga
//
LOCUS       DAEPOZ010000022        29034 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.022, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000022 DAEPOZ010000000
VERSION     DAEPOZ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29034
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.022"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            426..3086
                     /locus_tag="KR440_000799"
     CDS             426..3086
                     /locus_tag="KR440_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HBH0697862.1"
                     /translation="MKKILKRLCTGFLAFATVVTALPSSTVHASNTQYWTESKERVSI
                     VEQVMNDGSISSTFNEGHLTVEGEDAYCIDINTAFKNGYKTKADASTRMSADQIADVA
                     LSIEYVKQYTDIHSGISSKHAYLLRQLVVWQRLSVQPDWQWDNVRASYDEIPKAVQDE
                     VFAGAKAFVKENKGRYDCGGYIYSGEGQELGQFWAKLAVGNTKLQKTSTNANITDGNG
                     IYSIAGATYGVYSDKDCTKQLTTLTTDTSGNTEAVEVRATIVYIKELSAPAGFKIDKT
                     VYSLSVEVGKTATLKVSDTPKVTDTLIELFKIDMETQKSNPQGNASLEGAEFTWNFYA
                     GYYNKNNLPAQPTRTWVTKTIAEKDSDGAIHYITRLADKYKVSWDSFYTQDGKNVLPL
                     GTLTVEETKSPSGYLLEGTYMQADGSEEQIKGMYLTQITEDGDLAVLSGSNQYHVSDK
                     VIRGGVKIQKRDLETKDTKAQGGATLKDTAFEIISLNENAVLVEGKLYKKNEVLKTIH
                     TDIEGIASTSADLLPYGKYRLSEQKPPEGYLTDGAKPIDFEITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGSHKRLADVPFRITSKTTGESHVVVTDDNGQFSTASDWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALLYDTYIIEELRCESNKGFKLIPPFEIVISRN
                     KVVVDLGTLTDEYEKEITIHTTATSKDGEKTILAGKDVTITDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELIINDKRIENDYIFTADSETMEVKIEFTFDASNLGGKQLVTFEELYD
                     LSNPDEPIKVAEHKDIEDDGQTVTIKEVPETPTPEEPEKPTTPDTPTKTDSPKTGDNT
                     NILAFAIMMFVSAGGLAGTYFFKHRKMKKS"
     gene            3311..3634
                     /locus_tag="KR440_000800"
     CDS             3311..3634
                     /locus_tag="KR440_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBH0697863.1"
                     /translation="MELKYIVPNVAQTFGNLEFAGENKVDQQRINGRYTLVSRSYNLY
                     SDIQRADDIIVVLPAKAGEKHFKPEQKVKLINPRITAEGYNVKGRGFTNYILMADDML
                     PAESK"
     gene            3649..4032
                     /locus_tag="KR440_000801"
     CDS             3649..4032
                     /locus_tag="KR440_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBH0697864.1"
                     /translation="MRLTNGIVLDNEKTFGVLKFSALRHEVRVENEDGTTSDEIKRRT
                     YDLKCSVQERMIQVSIPADVPVKDFPYNAEVELINPVADTVANANFRGTTVDWYIKAD
                     DIVLKNKGGQAGNPQNNAPQGQQKK"
     gene            4128..4541
                     /locus_tag="KR440_000802"
     CDS             4128..4541
                     /locus_tag="KR440_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBH0697865.1"
                     /translation="MLDKIPNAEEMTALVGQSLYSVWNELCALIDEKYEMECLWNKGG
                     KAWNYEYKYRRGGKTLCALYARENCVGFMIILGKDERLKFDTYRDNYSKEVQRIYDET
                     KTYHDGKWLMFEPTDTTLFDDFVKLLRIKRKPNRK"
     gene            4648..5106
                     /locus_tag="KR440_000803"
     CDS             4648..5106
                     /locus_tag="KR440_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH0697866.1"
                     /translation="MKLKNPMLVVTDIDKSVEFYKKVFGLYVIMDFGANKTLTGGLAL
                     QTSETYKEFIGTSDISFGGNNFEVYFEEEDFDKFADRLKEYDIEYVHPIIEHSWGQRV
                     VRFYDPDKHIIEVGENMKIVCKRFLNSGMTPEQVAERMDVPMKFINACMR"
     gene            5131..5673
                     /locus_tag="KR440_000804"
     CDS             5131..5673
                     /locus_tag="KR440_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBH0697867.1"
                     /translation="MKGFERKNQLFSLCGLNCGLCPMLLGNYCGGCGNGNQSCRIAKC
                     SLEHGQIEYCYECKQYPCEKYQHIDDYDSFITHKRRKADLERAKNIGIEQYNLEQQEK
                     TQILSYLLSNYNDGRRKTFFCVAVNLLELSELQEAMKQIQSNDELTLLPFKAQCLYVV
                     EVFQKIAERRNIKLKLFKKK"
     gene            5709..6236
                     /locus_tag="KR440_000805"
     CDS             5709..6236
                     /locus_tag="KR440_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HBH0697868.1"
                     /translation="MTNGGIPLETVELRFQYTQSEYIKAERQYLISSKTIHKYDIALV
                     AVFLLLSVVYMLFSSFSVFSILIFGLIIVVTALGSYLYILMPILKFKQTAKYHEEYTL
                     VFSKENIKFKTQSIDSEVKWDIYSALWESHDFYYLIQAPRLYTLIPKRIFKDLNEKQL
                     FEEIAQSRVKTTKHI"
     gene            6346..7740
                     /locus_tag="KR440_000806"
     CDS             6346..7740
                     /locus_tag="KR440_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0697869.1"
                     /translation="MRMILNKGHRIRARDKNLVYHFSIGTLLFVFVAVLLLLNIKQLM
                     RTDWEHFSLLDNGLTLSPYNFITILIATGICALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMENGLLHIHCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRISIDEVKAENGCLRLMKNLV
                     WEYDVLPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEDMIDCVNAFYEGMVQRSEEMKQHPDYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGSLAFVRQ
                     DGTATCEAKGDGTD"
     gene            8020..9141
                     /locus_tag="KR440_000807"
     CDS             8020..9141
                     /locus_tag="KR440_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBH0697870.1"
                     /translation="MAGISREHLSRIEAGKVALTEDMKHKLLEAVEKFNPDAPMFLLF
                     DYVRIRFPTLDIQHVIKDILKLNIDYMLHEDFGHYKYTEHYYIGDVFVYTSQDKEKGV
                     LLELKGKGCRQFESYLLAQERSWYDFLMDALVDGGVMKRIDLAINDRTGLLDIPELIE
                     KCKNEECISKFRSFKNYGSGELVKHNEANKDGMGHTLYIGSFTSEVYFCVYEKNYEQS
                     SKLGIPIEEVPIKNRFEIRLKNEHAYYAIRELLTHYDAELTAFSIINQYIRFADKEAN
                     KRKSDWKTNARWSWFIGEGRPPVKLTTKPEPYTLQRTLNWLQRQVAPTLKMLKQIDAG
                     NQMDYLKTIEQQAKLTDKHKQIIRQQTASVDELIEKEKE"
     gene            9148..9282
                     /locus_tag="KR440_000808"
     CDS             9148..9282
                     /locus_tag="KR440_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBH0697871.1"
                     /translation="MWELIKDLLLVSLGMGIGVVLMCIMSVGKEADRHMIELKESEEK
                     "
     gene            9283..9504
                     /locus_tag="KR440_000809"
     CDS             9283..9504
                     /locus_tag="KR440_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697872.1"
                     /translation="MNFGQNLYTWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALVAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            9824..10462
                     /locus_tag="KR440_000810"
     CDS             9824..10462
                     /locus_tag="KR440_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase CAT"
                     /protein_id="HBH0697873.1"
                     /translation="MEYKYTKIDLNKWNRGKLFQSYIDNMRIVMSLTVDIDVTKFIEF
                     SKKHDLKFYPAMIWAVSKVVNEHDEFKYSWDNNGNLIKWDYISPSYTEFHKEDENFTK
                     IVTDFSDSIFEFHKRFILDREKYKTERAFVENQSLNFFDVSCLPWVKYNHFDVHVFDE
                     GKFLAPVITWGKYELEQERYIMPLTMNIHHAVADGFHLSRFFNEVQELINSI"
     gene            10539..10727
                     /locus_tag="KR440_000811"
     CDS             10539..10727
                     /locus_tag="KR440_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697874.1"
                     /translation="MKDIFSDMQASIGCTYISDLPHHKRMVWQEMKRLNLADYPQKQL
                     EDFAGYVFGVPYTSIAEA"
     gene            10748..11245
                     /locus_tag="KR440_000812"
     CDS             10748..11245
                     /locus_tag="KR440_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBH0697875.1"
                     /translation="MNEMRVCIESRLHTSEEIRSAWFTLPIDEEELEEQIGVPIDSED
                     YHIVEKELPFADEVGEDATIEKLNDLYHTFESLPADLQEDYGELMCYFTSLDELHQHR
                     HDVIHYSWCKNMADVARHLLDNDPAFSAIEERLTRYFDYEAYGQYLDDNGEFIETDHG
                     IYELP"
     gene            11261..11764
                     /locus_tag="KR440_000813"
     CDS             11261..11764
                     /locus_tag="KR440_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBH0697876.1"
                     /translation="MVEDMRVYIANLGKYNEGYLVGDWFSFPIDEEDVAERIGLNERY
                     EEYAVHDTENFPIEIGEYISIEELNEMYEMICELPDYITDSLDEFVSNYGSLEELYDH
                     RDDMILYAGCEDMTDVAYYFIDELQALGDIPPSLQNYIDYEAYGRDLSFEGTFIETRY
                     GMCEIPW"
     gene            11877..12266
                     /locus_tag="KR440_000814"
     CDS             11877..12266
                     /locus_tag="KR440_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH0697877.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDLPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITFALRPKITY
                     AGKAVKLDKQVLHEAITAVRSVSYVPD"
     gene            12253..14685
                     /locus_tag="KR440_000815"
     CDS             12253..14685
                     /locus_tag="KR440_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0697878.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSSRSIQEQSKKLVTGRLKDVAYQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTDQSMNLKSVKQSAFLTFKEFIHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLDKNDFGYLIEHIYGRDGVAYEDYEYSLPKKKLKKE
                     TLITHYDLIRPTRCLVEESQRHLRLEHEDSESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALSTVRNKKKELKDLDNHAYQSGSETSSNVVDALDSVD
                     ELETDLDQSRESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYIGYSVDTGRNVYLQPS
                     LASQGVKDTVTNALASAFVESLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDRENAGLLDPFVIMQNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRAVTQSDQRGLLHVIEELRKEDTPISCNIADHIDSFTDYDFAHLLFSDGTV
                     QNAISLDNQLNIIQVADLVLPDKDTTFELLSVSMLIVISTFALDFIHSDRSVFKIVDL
                     DEAWAFLNVAQGETLSNKLVRAGRAMNAGVYFVTQSSGDVSKESLKNNIGLKFAFRST
                     DINEIKQTLEFFSIDKEDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFEELLHAF
                     DTRPPIQRNEVE"
     gene            14685..16820
                     /locus_tag="KR440_000816"
     CDS             14685..16820
                     /locus_tag="KR440_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBH0697879.1"
                     /translation="MKERIKSVFTRKKIFRFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINTENLYSKYPLSNYQLDFYADNNWAWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     RLYLSNATGYVVQEAYKLDFINDMADSIGRSIQTLAGVTQNGFSTSGFYVGFLLLIIL
                     VVGIYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKVNDFSSDISTAS
                     LDLGTKIMLPDSDSKGKDSVDLIRDSLFSIQVQQPWLLLQFGNSGIEEIGADRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTILQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVYFAGIYMKLGDLMSMFSLNAGDSQSMGRRIFRRP
                     YLFMRHRARRMEHRLARAVSAGGVAGAVAGGVVASSKSTRQNGTKNNRGNTSSSMGQK
                     AGSAVGAVLDTKNKVKDKATAVKENIKDMPTQTAYAVHSAKEKAKSSVSDFKRGVVQE
                     KENRQSGRLKKQQLHRQNIADKRMELQKAQESRQAGKKTDGSATSGATRPHERPATTS
                     KVNVEKQQEVKRPAIASTVKNDEPIKKNVVKERPLSSGDTSAKTVNQETTRQNYKTDR
                     VTKTTVQKHTQQNTEKNKRTVFKKGQKKK"
     gene            16817..17824
                     /locus_tag="KR440_000817"
     CDS             16817..17824
                     /locus_tag="KR440_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBH0697880.1"
                     /translation="MKLRHIAIVGSLFSILFSLVLFFGVLISAEDDDGGNGYSSNYVG
                     MNLSAEVLKHQPMVEKYAKENGISEYVNVLLVIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGISDINVVIQSYNYGGGYVGYVAGNGKKHMFNLA
                     ENFARDKSGGQKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLVVNQVSGELAQKVM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLVQWCYNKAGVSLPRTAQNQYDATQHIPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGIYVGNTQMYHAGDPIGYADLNNAYWQQYLICAGRIK
                     Q"
     gene            17840..18733
                     /locus_tag="KR440_000818"
     CDS             17840..18733
                     /locus_tag="KR440_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBH0697881.1"
                     /translation="MFKKKEKPVKETKVRTMKVGTHKKSVIALWVVLIASVSFGVYKN
                     FTAIDMHTVHETETIQLRLNDTNGVENFVKNFCKSYYTWDNSKEAIEARTQAISNYLT
                     KELQDLNLDTIRTDIPTSSTVTNVLIWDIKQSGTNDFIATYEVDQQIKEGEQTSNVKA
                     TYTVKVHVDKDGDMVIVQNPTIAPAIEKSDYEPKAQEADASVDADTVNDATAFLETFF
                     KLYPTATEKELAYYVAGNVLEPIGRDYLYSELVNPMFTDDRDNVKVRVAVKFIDNQTK
                     AMQVSQFELVLHKDSNWKIIM"
     gene            18946..19803
                     /locus_tag="KR440_000819"
     CDS             18946..19803
                     /locus_tag="KR440_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0697882.1"
                     /translation="MYKEVGISSNNVPITLSIWYTERNSPTIVFLPGTMSHPLMYENF
                     LCGLSERGFNIIGIHYLSHGKSPKIKKNYTMEDLLHNVYDATTYGIENFGENIAVMGS
                     SQGGILAAMAAGRDTRLKAVFPHNIMLTTLKETMSLTKYPAWSHRFMGLIQLGFRVGG
                     KLLPNYKVPGDAYLDYDRVFYSEQAKQDCLNDPMLLPYYPLRFVASLFNADLSCLENG
                     AIQCPVILITAKGDPLFRFDYTNKVFDLIHVPKKELLTLELNRHMIFNEDTDTTINAL
                     EATLHKYLG"
     gene            19881..20462
                     /locus_tag="KR440_000820"
     CDS             19881..20462
                     /locus_tag="KR440_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0697883.1"
                     /translation="MPRFTEQEKEIINSKLLIEGEKLFSLYGLKKVTVDDLAAAVNIS
                     KGSFYAFYPSKEHLYVEINFRLQKELFTSIETTVKKKKYKNHRDLAKDVIMLSLTGVI
                     TSPILSQIDLSLMDYLQRKISSDIFENHMYSDIRILEILEGLGVVFLVPHTVIIKSLY
                     SVLSCLEQFKEDEELNMVQNLLVNGIIQQVVAE"
     gene            20566..21411
                     /locus_tag="KR440_000821"
     CDS             20566..21411
                     /locus_tag="KR440_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0697884.1"
                     /translation="MIDVKKLYFSYTDKPFVENVSFHVGRGEIFGFLGPSGAGKSTIQ
                     KILTGLNTSYKGSVKVAGTEIREHTNRFYENIGVDFEFSTCYEKFTARQNLAYFASLY
                     DKQPRSIDELLHMVGLENDGDKKVADFSKGMRSRLNFIKALVHDPDILFLDEPTSGLD
                     PTNNRLMKDIILAEKKRGKTIIITTHNMYDATELCDQVAFIVAGKVSALDSPHNLIMS
                     RGAAKIHYTYYDKGEKTGECLLDRTTEDKLLKSLISENRLLSIHSSEPTLNDIFVDIT
                     GRTLQ"
     gene            21408..22091
                     /locus_tag="KR440_000822"
     CDS             21408..22091
                     /locus_tag="KR440_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0697885.1"
                     /translation="MRLGRLICGDIHFQWKYGFYFIYFILTVLYVCGIAALTGHWKTD
                     IASIMIYSDPAAMGLFFMGAIVLLEKSQKVLNAMVVSPVKVSEYILSKTIALIAISTI
                     IAVILGFVSGSNHLLGIAVGTALTSAIFTMLGIIAATKISNLNQFLIVIMPIEIVCFV
                     PPIVGLFVKLPDIFRFFPFTACMNLITGKSILLSFDMVLVIATLIILYIVARHTVKHM
                     WRSLGGVKL"
     gene            22088..22786
                     /locus_tag="KR440_000823"
     CDS             22088..22786
                     /locus_tag="KR440_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0697886.1"
                     /translation="MKRILSSTIQVFKQIKSDPMMFAACFTPFVMGALIKFGIPFLDG
                     ITDFSLQTYYPIFDLLLSIMAPVLLCFAFAMITLEEIDDKVSRYFSITPLGKSGYLFT
                     RLGVPSIISAVIAFIVLLLFSLEKLSIGMTICLALLGSVQAIIVSLMIITLSSNKLEG
                     MAVTKLAALTLLGIPAPFFIDSYYQFAVGFLPSFWVAKAMQNEAVLYFSIGLMVALVW
                     YYFLIKRLFRKLAG"
     gene            22798..23709
                     /locus_tag="KR440_000824"
     CDS             22798..23709
                     /locus_tag="KR440_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBH0697887.1"
                     /translation="MEKGRLLQNENINKAIQVSKITLIINLGLSLLKFAAGYIGKSSA
                     MLSDAVHSASDVLSTIVVMVGIKISEKQPDKEHPYGHERMECVASIILSVALAITGAG
                     IGYSGIKKIFSGQYNTLSVSSGIALTAAVLSIVIKEWMYWYTRSAAKHTNSDALMADA
                     WHHRSDALSSVGSLIGILGARLGYAILDPIASIVICGCILKAALDIFKESINKMVDHS
                     CDNATETKIREVVLQQQGVDGIDELKTRMFGAKMYVDIEILADGNLALYDAHRIAEGV
                     HQTIENNFPQCKHCMVHVNTNELPYSP"
     gene            23762..23902
                     /locus_tag="KR440_000825"
     CDS             23762..23902
                     /locus_tag="KR440_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697888.1"
                     /translation="MEQYWWIIIVMVLIVVGLLKIVIMKKYNQRRNSNNRSKNSEDDK
                     MY"
     gene            24017..24208
                     /locus_tag="KR440_000826"
     CDS             24017..24208
                     /locus_tag="KR440_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HBH0697889.1"
                     /translation="MKKYEYVEINYNAKGVVFLCTDKHKEIIDSYAKTGYHYAGFVPT
                     EIDAKGCMRKIDLIFEKED"
     gene            24232..24462
                     /locus_tag="KR440_000827"
     CDS             24232..24462
                     /locus_tag="KR440_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697890.1"
                     /translation="MIGLKRRDMNYIMKVGAKAQGVSVSEFRAMLQDTIDKTMESTDP
                     DVQANFKRYFGNKRPTPEEYIYTITKKTKVKV"
     gene            24728..25018
                     /locus_tag="KR440_000828"
     CDS             24728..25018
                     /locus_tag="KR440_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697891.1"
                     /translation="MKPSDFQKTIQCQFECKLKKVVKGIIWNYRKELRRRKNKEISFS
                     ELPDITVESLATWDDYETNYTIFNVCGIDIKVLDDELAEALKQLPEKNVTMY"
     gene            25018..25152
                     /locus_tag="KR440_000829"
     CDS             25018..25152
                     /locus_tag="KR440_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH0697892.1"
                     /translation="MYYFLEMSDTEIADIMNYTREAVFKHRHRALETMKEILNEKTRQ
                     "
     gene            25133..26314
                     /locus_tag="KR440_000830"
     CDS             25133..26314
                     /locus_tag="KR440_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBH0697893.1"
                     /translation="MKRQDSKGRNLRLGESQRKDGRYVYKYTDIFGKPQFVYSWKLVP
                     TDKTPAGKRDDISLREKEKQIKKDLDDGIDTVGGKMTVCQLYEKKNNQRKNIKRNTEL
                     GRQYLVNALENDPLGMRAIDTVKQSDAKEWAIRMNGKGYSYKTIDNYKRSLKASFYMA
                     IQDDCIRKNPFNSKLSDVLEDDTEAKVILTPEQEEKLLAFMETDNVYSKYRDEVILLL
                     ETGLRISELCGLTTHIDMLNRVINIDHQLLRDTEVGYYISKAKTKNGKRELPLTERAY
                     QALKRILKNRGKAQPLVVGGYSNFLFLNREGLPKVAGNYESMVRGLIKKYNKTHKDKL
                     PNITPHSFRHTYCTNMANKGMNPNTLQYIMGHANITMTLGYYAHGTFQSAKAELERLA
                     S"
     gene            complement(26654..27466)
                     /locus_tag="KR440_000831"
     CDS             complement(26654..27466)
                     /locus_tag="KR440_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0697894.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRIL"
     gene            complement(27542..28777)
                     /locus_tag="KR440_000832"
     CDS             complement(27542..28777)
                     /locus_tag="KR440_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBH0697895.1"
                     /translation="MGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITSGVTGMF
                     CHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLRGSLASLLEVESGSNDPIAY
                     MLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFYTIF
                     ITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIMLFF
                     MLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVGLRG
                     AASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTPVLK
                     TFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGSTII
                     KTGDILVLSGDRIEELIQL"
     gene            complement(28925..>29034)
                     /locus_tag="KR440_000833"
     CDS             complement(28925..>29034)
                     /locus_tag="KR440_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH0697896.1"
                     /translation="REWICPSCNETHDRDINASINILKEGLRLITIQNK"
BASE COUNT         9460 a         5194 c         6064 g         8316 t
ORIGIN      
        1 tactttatat ccttaaaaaa atatacaact ttatatataa aacatcaata tttatatata
       61 ttttgtatat taaatacaat tatttataaa atctttgata aaaatttata aatagagcat
      121 ctttagaaaa ctattaacag aaaaacacac taaaaatttg attatccgct aaaacactgt
      181 atctatcggc attatcaagg agctaaacac atcaaaatac tactaattta ctacgattga
      241 atgagccttg atacgtccta tttcccagat atgcaaagat acggtacagc acatcagtat
      301 tgaaacaaga aaaattgaat acttcataga ctacggaacg cacaatatca gcgttccttt
      361 tctatttcca gccgttccta gtggacggct attttactac caaaatgaaa ggagcattta
      421 gaattatgaa aaagatatta aaacgattgt gtacaggctt tcttgccttt gcaactgtcg
      481 ttaccgcttt accgagttca acggttcatg cgtctaatac acagtattgg acggaaagta
      541 aagagcgtgt cagtatcgta gaacaagtaa tgaatgacgg ttctatcagt tctaccttta
      601 atgagggaca tttaaccgtt gagggtgagg acgcttattg tatcgacatc aacacagcct
      661 ttaaaaacgg ctataagacc aaagctgacg caagtacccg tatgagtgcc gaccagatag
      721 ctgacgtcgc cttatctatt gagtatgtga aacagtacac agacatccac agcggaataa
      781 gcagtaaaca cgcctaccta ttaagacagc ttgttgtctg gcagagatta agtgttcagc
      841 ctgactggca atgggataat gtgcgagctt cctatgatga aatcccaaaa gccgtacaag
      901 atgaagtatt cgcaggagca aaagcctttg tgaaagaaaa caaaggacgt tatgactgtg
      961 gcggatatat ctactctggc gagggtcaag agttagggca attctgggcg aagttagctg
     1021 tcggaaatac caaacttcaa aagacttcca ccaatgccaa tattacagac ggcaacggga
     1081 tttattcaat cgctggtgca acatacggtg tgtattctga taaagactgc acgaaacagc
     1141 ttaccaccct tacgactgat actagcggaa atacagaagc cgttgaagta agagccacta
     1201 tagtatatat caaggaactt tctgcaccag caggatttaa aattgataaa acagtttact
     1261 ctttatctgt ggaagttgga aaaacagcga ctttaaaagt atctgataca ccaaaagtaa
     1321 cagatacctt gattgagcta ttcaagattg acatggaaac acagaaatcc aatccgcagg
     1381 gaaacgcttc tttagaggga gcagagttta catggaactt ctacgctgga tattacaaca
     1441 aaaacaatct tccagcacag cctacaagaa catgggtaac aaagactatc gctgaaaaag
     1501 acagcgacgg tgctatccat tacattactc gtttagctga caaatataaa gtatcttggg
     1561 acagcttcta tacgcaggac ggtaaaaacg tacttccatt aggaacacta actgttgagg
     1621 aaacaaaaag tccgtcgggt tacttgttag agggtacata tatgcaggct gacggtagcg
     1681 aagaacagat aaaaggaatg tatctgacac agattacaga ggacggcgac cttgccgtat
     1741 tatctggaag caatcaatac catgtttctg ataaagtcat tcgtggcggt gtgaaaatcc
     1801 agaaacgaga tttagaaacc aaagacacga aagctcaagg cggtgcgact ttgaaagata
     1861 ccgcctttga aatcatttcc ttaaatgaga atgccgtctt agttgagggc aagttataca
     1921 agaaaaatga agtgttaaag acaatccata ccgacattga gggtatcgct tctacttctg
     1981 ctgacctatt accttacgga aaatacagat tgtctgaaca aaaaccgcca gagggatatt
     2041 tgactgacgg tgcaaagccg attgattttg aaatcacaga aaatggaaaa atcgttgatt
     2101 tgactgacga agcccactct atctataacc agattaaacg tggcgatatt gagggcgtaa
     2161 aaattggtgc aggttcgcac aagcgtcttg cagatgttcc gtttaggatc acaagcaaga
     2221 cgacgggcga aagtcatgtt gttgtaactg atgataacgg acaattctct actgcttctg
     2281 attgggcttc ccacaagcac aataccaatg caggaaaaac aagcgaggac ggtgtgtggt
     2341 ttggtacttc tgaaccagac gacagcaaag gtgcgttgct ttatgatact tacatcattg
     2401 aagaattacg ttgtgaaagc aacaaaggtt tcaaacttat tccaccgttt gagattgtca
     2461 tttccagaaa taaggttgtg gttgatttag gtacgctgac tgatgaatac gaaaaagaaa
     2521 tcactatcca tactactgct accagcaagg acggcgaaaa gactattctt gctgggaaag
     2581 acgtaactat cactgataca gttaaattag acggtcttac caaagggaca aaatatcagt
     2641 tgaaaggctg gcagatgtta aaagaagaaa atgcagagct tatcatcaat gacaagcgta
     2701 ttgagaatga ctacatcttt acggctgaca gcgaaactat ggaagttaaa atcgagttta
     2761 cctttgacgc ttccaatctt ggcggtaaac agcttgtaac ttttgaggaa ttatacgatt
     2821 taagcaatcc agacgaacct atcaaagttg cggaacacaa ggacattgag gacgacggac
     2881 agacagttac tatcaaggaa gtaccagaaa cacctacccc agaagaacca gagaagccaa
     2941 ccacaccaga tacacctacg aaaacagaca gtcccaaaac gggcgacaat acaaatatcc
     3001 ttgcctttgc aattatgatg tttgtttctg ctggtgggct ggctggtaca tactttttca
     3061 aacaccgtaa gatgaagaaa tcataaggcg gtagctgtat gagaaaagaa aaatcacgct
     3121 ctccgccata gcttagatag gcagactata cctattctgg cttgtctgcc gattacctgc
     3181 aagaacacgg atagtcaagg gtgcgttagc atttatttac cctttactat ctgggggctt
     3241 gctggcactt tcctaaatgc cagaatacac acatcaatca aacttaaagt aagaaacgag
     3301 gtaaaataat atggaattaa aatatatcgt gccgaatgtg gcacagactt ttggaaactt
     3361 ggaatttgca ggggaaaaca aagtagacca gcaacgtatc aatgggcgtt acactctcgt
     3421 atcaagaagt tacaacttgt attcggatat tcagagagcc gacgacatta tcgttgtatt
     3481 gcctgcaaaa gctggcgaga agcattttaa gccagagcag aaagtaaaac tgattaaccc
     3541 acgcattacc gcagagggtt acaacgtcaa aggacgtgga tttacaaact acattttaat
     3601 ggctgatgat atgttaccag cagaaagcaa ataagggggt atttgattat gagattaaca
     3661 aatggaattg tattagataa cgaaaaaact tttggagtat tgaaattctc tgcacttcga
     3721 catgaggtgc gtgtagaaaa tgaggacgga acgacaagtg atgaaattaa gcgtcgtact
     3781 tatgatttga aatgtagtgt gcaggaacgc atgatacaag tgtcaatccc cgctgatgtt
     3841 cccgtaaagg actttcctta taatgcggaa gtggagttaa tcaatcccgt agctgatacg
     3901 gtggcaaatg ccaactttag agggactacg gttgattggt acatcaaagc tgatgatatt
     3961 gtattgaaga acaaaggcgg tcaagctgga aatccgcaga acaacgctcc gcagggacag
     4021 cagaaaaagt aagctgacaa actagttttt cattgtagga gatataaaca actgttataa
     4081 tatggatgtg gaaacagtta atttagactt ataaaggaga aatgtgtatg ctagataaaa
     4141 tacctaatgc agaggaaatg actgctttgg tcggacaatc attatatagt gtatggaatg
     4201 aattgtgtgc cttgattgat gaaaagtatg aaatggaatg tctatggaat aaaggtggca
     4261 aagcatggaa ttatgaatat aagtatcgtc gaggtggcaa aacattatgt gccttatatg
     4321 ctagagaaaa ttgtgtggga tttatgatta tcttaggtaa agatgaacgg ttaaaattcg
     4381 atacatatag agataattat tcaaaagaag ttcagagaat ttatgatgaa acaaaaactt
     4441 accatgacgg aaaatggctg atgtttgagc caacagatac aactttgttt gacgattttg
     4501 ttaaactttt gaggataaaa agaaaaccaa atagaaagta gtttattacc acaccattag
     4561 caaatggata gtttcttata atagcggata tatgaaaatg ctgtcatgtg gacataaaga
     4621 aaattggaag ttgtggaggt agcaaaaatg aaattaaaaa accctatgtt ggtagtaact
     4681 gatatagata aatcagttga gttttataaa aaggtttttg gactttatgt cattatggat
     4741 tttggggcaa ataaaacttt aacaggtgga ttagccttac agacaagtga aacatacaaa
     4801 gagtttatcg gtacaagtga tatttctttt ggtggtaaca attttgaagt ctattttgaa
     4861 gaagaagatt ttgataaatt tgctgatagg ctgaaagaat atgatattga atatgtccac
     4921 cctattatag aacattcatg gggacagcgt gtagtccgct tttatgaccc agataaacat
     4981 attatcgaag ttggagaaaa catgaagata gtgtgcaagc gtttcttaaa tagtggaatg
     5041 acaccagaac aagttgcaga acgtatggat gtacctatga aatttataaa tgcttgtatg
     5101 cgataaattg aaagttagga gactaatact atgaaaggat ttgaacgcaa aaatcaatta
     5161 ttttctcttt gcggattgaa ttgcggacta tgccctatgc ttttgggaaa ttactgtggc
     5221 ggttgtggca atggtaatca atcatgcaga attgcaaaat gtagtcttga acatggacag
     5281 attgaatatt gctatgaatg caaacaatat ccatgcgaaa aatatcaaca cattgatgat
     5341 tatgattctt tcatcacgca taaacggcga aaagcagatt tagaaagagc aaaaaacata
     5401 ggcattgagc aatataatct tgaacagcag gaaaaaacac aaattctttc ttacttactt
     5461 tctaactata atgacgggcg tagaaagact ttcttttgtg tggcagtcaa tttattagaa
     5521 ctttcagaat tacaagaagc aatgaaacaa atacaatcca atgacgaatt gaccttacta
     5581 ccatttaagg cacaatgctt gtatgtggtg gaggtgtttc agaagattgc tgaaaggaga
     5641 aatataaaac tgaaactttt caagaaaaag tgattttcaa atttgaaatt gtaacaaaga
     5701 ttatttatat gacgaatggg ggaatacctt tggaaacagt agagttaaga tttcaatata
     5761 ctcaaagtga atatataaaa gcagaaaggc aatatttaat ttctagtaaa actatacata
     5821 aatatgatat tgctttagtc gctgtatttt tgttgctgtc agtcgtttat atgctgttct
     5881 cctcttttag cgtatttagt attttaatat ttggattgat aattgttgta actgcattgg
     5941 gaagctattt atatatccta atgcctatac tgaaattcaa acaaactgca aaatatcatg
     6001 aggaatatac gctggtattt tctaaggaaa acattaaatt taaaacacaa agcattgact
     6061 cagaagtgaa gtgggacatt tactctgcac tatgggaaag ccatgatttt tattacttaa
     6121 ttcaagcacc acgattatat acgttaattc ccaaaagaat ttttaaagac ctaaacgaaa
     6181 aacagctatt tgaagaaatt gcacaatcaa gagttaaaac aacaaagcat atataatgaa
     6241 cataccctac aatgtaacaa cttcatattc atacacacaa agcggttagt cttttaagat
     6301 tgactgcttt tttcatactt aaattaagat tggagtgatt gatttatgcg aatgattttg
     6361 aataaaggac accgtatcag agccagagac aagaaccttg tctaccactt ttccataggg
     6421 acgcttctgt ttgtatttgt ggcggttctc ctgctactga atatcaaaca gctcatgcgt
     6481 accgactggg aacatttcag cttattggac aatggcttga cgctttcccc ctacaacttc
     6541 ataaccatat tgatagcgac tggtatttgt gcattggtcg cttttctgta ttaccgcttc
     6601 tgttatgaca gtttcaaaaa gctcctgcac cgtcaaaagc tggcacgaat gatactggaa
     6661 aacaagtggt atgaagcaga taccgtacaa gacagcggtt tttttactga cctgcaaagc
     6721 agatcaaggg aaaaaatcgt ctggtttcca aagatttatt atcaaatgga aaacggattg
     6781 cttcatattc actgtgaaat cacattagga aaatatcaag accagctttt gcgattagag
     6841 gataagctgg aaagcggttt gtactgtgag ctgaccgaca agaccttgca tgacggctat
     6901 attgagtaca cgcttttata tgacatgata gcgaaccgta tttccattga cgaagtgaaa
     6961 gcagaaaacg gctgtcttag gctgatgaaa aatcttgtct gggaatatga cgtactccct
     7021 cacgctctga tagctggtgg gactggtggc ggtaagacct actttctgct gacgcttatt
     7081 gaagctctgc tacatacgaa tgctgtcctt tacatcttag acccaaagaa cgctgacctt
     7141 gcagacttag gtacagttat gggaaatgtg tatcatacca aagaagatat gattgattgt
     7201 gtcaatgcct tttatgaggg catggtacaa agaagcgaag aaatgaagca acacccagac
     7261 tataaaacgg gcgaaaacta cgcctatctt ggtttaccgc cctgctttct tatctttgat
     7321 gaatatgtgg cgttttttga aatgctgggg acaaaggaaa gcgtaagttt acttagccag
     7381 ttaaagaaaa tcgttatgtt gggacgacaa gctggatatt tccttattgt tgcctgccag
     7441 cgtccagacg caaaatattt ctcggacggt atcagagata atttcaattt ccgtgttgga
     7501 ttggggcgta tcagcgaatt gggttacggt atgctctttg gttctgatgt gaaaaaacag
     7561 tttttccaga agcgtatcaa ggggcgtggc tattgtgatg tgggaacaag tgtcataagt
     7621 gagttttaca cgcctttagt cccgaaagga catgattttt tgcagaccat aggctctctt
     7681 gcattcgtaa ggcaggacgg gacggcgacg tgcgaagcga aaggcgacgg cacggactag
     7741 ccgttgctgg tgtggcgtta gccacgccag cagatataag cccctcatta tctaacagag
     7801 gggcacaatt acgctggaaa ctggttcaaa aaccttgaaa agtccgctag gaataaggat
     7861 tttcttagtt tttagctagt tgtgataatc acttgaaaat atcacaaaat cacaacaata
     7921 tcacgattgg gggtatgaca actgaatgat actgattttc tgaacacctt gaaagcaaaa
     7981 cgcagtgatt atggcgtatc gcagacaagg cttgcaatca tggctggtat cagccgtgaa
     8041 catctaagcc gtatcgaagc tgggaaggta gcactgaccg aggatatgaa gcacaaacta
     8101 ctggaagcgg tggaaaaatt caatcccgac gctcccatgt tcctgctctt tgattatgtg
     8161 cgtatccgat ttcccacgct ggatatacag cacgttatca aggacattct aaagctgaat
     8221 atagattata tgcttcacga agatttcggg cattacaagt acaccgagca ttactatatc
     8281 ggggacgtgt tcgtttacac ttcacaagac aaggaaaaag gtgtcctgct ggaactgaaa
     8341 ggaaaaggct gtcgtcagtt tgaaagctat ctgctggcac aggaaagaag ctggtatgac
     8401 ttcctcatgg acgcattagt tgatggcggt gtgatgaagc gtatcgacct agctatcaac
     8461 gacagaacgg gcttactgga tattccagaa ctgatagaga aatgcaagaa cgaggaatgt
     8521 atttctaaat tccgtagctt caagaactat ggttctgggg agctggtaaa gcacaacgaa
     8581 gccaacaagg acggcatggg acatacgctt tacattggtt catttaccag tgaagtgtac
     8641 ttctgtgtct atgaaaagaa ttatgaacaa tcctcaaaat tagggatacc gattgaggaa
     8701 gtccccatta aaaaccgatt tgagatacgt ttgaaaaatg agcacgccta ctatgccatt
     8761 agagagctgc tgacccacta cgacgcagag ctgacggcat tttctattat caaccagtat
     8821 atccgctttg ccgataagga agccaacaag cgaaaaagcg actggaaaac caacgcccgc
     8881 tggtcttggt tcattggcga gggacgaccg cccgtgaagc tgacgacaaa gccagagcct
     8941 tacactctgc aaaggacgct gaactggtta caaagacagg ttgcaccgac cttgaaaatg
     9001 ctgaaacaga tagacgctgg caatcagatg gactatctca agaccattga acagcaggca
     9061 aagctgacag ataagcacaa gcagataatc agacagcaga cggcttcggt ggacgaactg
     9121 atagaaaaag aaaaggagtg attgatgatg tgggaattga ttaaggactt actgctggta
     9181 tcacttggca tgggtatcgg cgtggttctg atgtgtatta tgagtgtcgg aaaggaagct
     9241 gaccgacaca tgattgaatt aaaagaaagc gaggaaaaat aaatgaattt cgggcaaaat
     9301 ttatatacat ggtttttaag caacgcccag agcttagtgc ttatggcgat tgtggttatt
     9361 ggtatctact tagggtttaa acgtgagttt tcaaaactta tcggcttctt ggtggttgcc
     9421 ttagttgctg ttggattggt attcaatgca ggcggtgtca aagacgtact cttagaacta
     9481 ttcaataaga ttatcggcgc atagccgtac tttttcatta tggtttgcgg atacgcaaac
     9541 atacctttcc cactttggga gtgggctaag gggaaagggg gtaacggaat ggtacacaga
     9601 taaaaataca gcataatgaa tttcattgct tggctcaaac atagttgata taataaagat
     9661 atgaaaataa taaacgaaag tttttttgat aaggagtgat attgtgaaat tgtgtattgt
     9721 tttatctcca tataaggctt aatggaatag acttgtatgg agtgaaatgt tatccagaga
     9781 gccttacatt ttatcaaatt tcaaaatgta aaggagaatg ggcatggaat ataaatatac
     9841 aaaaattgat ttgaataaat ggaatagagg taaattgttt caaagctaca ttgataatat
     9901 gcgtattgtt atgagcttaa ccgtagatat tgatgtaaca aagtttattg aattttcaaa
     9961 gaagcatgat ttaaaatttt atccagctat gatatgggct gtttcaaagg ttgttaatga
    10021 acatgatgag ttcaaatata gttgggataa taatgggaat ttaattaagt gggattatat
    10081 ttctccgtcc tatactgaat ttcataaaga agatgaaaat ttcacaaaga tagttacaga
    10141 tttttctgat agcatatttg aatttcataa acgttttata ttagataggg agaagtataa
    10201 aacagaacgt gcatttgtgg aaaatcaatc tttgaacttt tttgatgtat cttgtttacc
    10261 gtgggtaaaa tataatcatt ttgatgttca tgtttttgac gagggaaaat ttcttgcacc
    10321 agttattact tggggcaaat atgaattaga gcaagaaaga tatataatgc ctcttacaat
    10381 gaatatacat catgccgtag cagatggatt ccatttatcc agatttttta atgaagtaca
    10441 agaattgata aactcaattt aacagctata tacaaagcag ttagtctata cgattgactg
    10501 ctttttttca tacctaaatt taagattgga gtgattgctt gaaagatata ttttcggata
    10561 tgcaggcaag tatcggctgt acctacattt cagatttacc acatcataaa cgtatggtgt
    10621 ggcaggaaat gaagcgactg aaccttgccg attatccgca gaaacaatta gaagattttg
    10681 caggctatgt ctttggtgtc ccttacacta gcatagctga agcatgaaag aaaggacgtg
    10741 aaaacctatg aatgaaatgc gtgtctgtat cgaaagccga ctacacactt ctgaagaaat
    10801 ccgttccgca tggttcacac ttcccattga cgaggaagaa ctggaagaac agataggcgt
    10861 tcctatcgac agtgaagatt accatatcgt tgaaaaggaa ctgccctttg ctgatgaagt
    10921 cggcgaagat gcgaccattg aaaaactgaa tgacctttac catacctttg aaagtctgcc
    10981 tgccgattta caagaggact acggggagct gatgtgttac tttaccagcc ttgacgaact
    11041 gcaccagcac cgccatgatg tgatacatta ttcatggtgt aaaaacatgg ctgatgtggc
    11101 aaggcatttg ttggacaatg accctgcttt ttctgccatc gaggaacgcc tgacccgtta
    11161 ctttgattat gaagcctacg gacagtatct tgatgataac ggagaattta tagaaacaga
    11221 ccacggtatc tatgaactgc cgtagaagtg aggtgtttct atggtagagg atatgcgtgt
    11281 atatatcgct aatcttggga aatacaatga ggggtatctg gtcggcgact ggttctcttt
    11341 tccgattgac gaagaagatg ttgccgagcg tatcggcttg aatgaacggt acgaagaata
    11401 cgccgtacat gatacggaaa actttcccat tgagattggc gaatatatct cgattgaaga
    11461 gctcaatgaa atgtatgaaa tgatatgtga gcttcccgac tacatcacgg acagtttgga
    11521 cgaatttgta tcaaattatg gaagtttgga ggaactgtac gaccataggg acgatatgat
    11581 tttatacgct ggctgtgagg atatgaccga cgttgcctat tacttcattg atgaattgca
    11641 ggcacttggc gatattcccc ctagtttgca gaactatatt gactatgaag catacggcag
    11701 ggatttatcc tttgagggaa cttttattga aacaagatac ggtatgtgtg agataccgtg
    11761 gtaagaagct ggaagatcag cgacaagcat tgttgcttct gacagctttt tctattgggg
    11821 acagtctgaa aaacggctgt ccttttctat tatacgaaag gagttgaaac aaaaacgtga
    11881 aaaagattaa aagctacacg ggtatctgga acgtggaaaa agtcttgtat gcaatcaatg
    11941 actttaacct gccctttcct gttaccttta cacagattac atggtttgtg ataaccgagt
    12001 ttatcatcat tctgtttggg gacttaccgc ccttatccat gattgagggg gcatttttaa
    12061 aatacttcgg tattcccgta gctcttacat ggttcatgtc gcaaaagacc tttgacggca
    12121 agaagccgta cagctttttg aaatcacaga taacctttgc cctacgacca aagattactt
    12181 atgcaggaaa agccgtgaaa ctggataagc aggtactaca tgaagctatt acggcagtaa
    12241 ggagtgtgag ctatgttccc gattaaatat atcgacaaca accttgtctg gaacaaggac
    12301 aatgaggtgt tcgcctacta tgagctgata ccttacaatt acagttttct atccgctgaa
    12361 cagaaattca ttgtgcatga cagcttccga cagctaatcg cacaatcccg tgagggcaag
    12421 atacacgctt tgcagattgc cacagaaagc tcaagccgaa gcatacaaga acagtcaaag
    12481 aagctggtta cgggacgact aaaagacgtt gcctatcaga agatagacga acagaccgaa
    12541 gcgttagtat ctatgattgg ggacaatcaa gtggactacc gcttttttct tggcttcaag
    12601 ctcatggtaa cagaccagag catgaaccta aagagcgtga agcaatcggc gtttctcaca
    12661 ttcaaggagt ttatccatga agtgaaccac acgctgatga atgactttgt ttccatgcca
    12721 aatgatgaaa tcaaccgcta catgaaaatg gaaaagctgt tggaaaataa aataagccga
    12781 cgcttcaaag taagacgctt agataaaaat gacttcggct atctgattga acacatctac
    12841 ggcagggacg gtgtcgccta tgaagattat gagtatagct taccgaagaa gaaactgaaa
    12901 aaggaaacgc tgataacgca ttatgacttg atacgtccga cccgttgcct tgtggaagaa
    12961 agccagcgac atttacgctt ggaacatgag gacagcgaaa gctacgtttc ctactttact
    13021 gtcaatgcga ttgtcggcga gcttgatttt ccgtccagtg aaatcttcta tttccagcaa
    13081 cagcagttta cttttcccgt tgataccagc atgaatgtag aaatcgtgga aaaccgaaaa
    13141 gcgttatcga cggttcgcaa caagaaaaaa gagctgaaag atttagacaa tcatgcatac
    13201 cagtctggca gtgaaaccag ctcaaatgtg gtagacgcat tagacagtgt ggacgagctg
    13261 gaaacggatt tagaccagtc cagagaaagt atgtataagc tctcttatgt tatccgtgta
    13321 tctgctccag accttgacga gttgaaaaga cgctgtgatg aagtcaaaga cttctacgac
    13381 gatttgaacg tcaagctggt acgccctgct ggcgatatgc tggggcttca ctctgaattt
    13441 cttcctgcca gcaagcgata catcaacgat tatgtgcagt atgtcaaaag tgattttcta
    13501 gctgggctgg gctttggtgc gacacagcag ttaggggaaa acacgggtat ctatatcggc
    13561 tattctgtcg atacgggacg caatgtttat ctacaaccgt cccttgccag tcaaggcgta
    13621 aaagatacgg ttactaatgc tcttgcttct gcctttgtag aaagtcttgg cggtggaaaa
    13681 tcattctgta acaacctgct tgtctactac tctgtgctgt ttggcggtca agcggtaatc
    13741 ttagacccaa aatccgagcg tggcaactgg aaagaaacgc tcccagaaat cgcccatgaa
    13801 atcaatatcg tgaaccttac cagcgacaga gaaaatgcag ggcttcttga cccgttcgtg
    13861 attatgcaga atgtgaaaga cgctgaaagt ctggcaatcg acatactcac attccttacg
    13921 ggcatttctt caagggacgg cgagaagttc cccgtactta gaaaggcagt gcgtgccgtt
    13981 acccagagcg accagcgagg attgctccat gtgattgagg aattaagaaa agaagataca
    14041 cctatctctt gcaatattgc cgaccatatc gacagcttca cggactacga ctttgcacat
    14101 ctgctgttct cggacggtac ggtgcaaaat gcaatcagct tagacaacca gctcaatatc
    14161 attcaagtag ccgacttagt gctacccgat aaggacacga cctttgaatt actgtccgtt
    14221 tccatgctga ttgtgattag tacctttgcg ttagatttta tccactcgga tagaagtgtt
    14281 ttcaagattg tagaccttga cgaagcatgg gcgttcttaa atgtggcaca aggcgaaacg
    14341 ctctctaaca agctggttcg tgctggacgt gccatgaacg caggcgttta ttttgttaca
    14401 cagtcctctg gcgacgtgtc aaaggaaagt ctgaaaaaca atatcggttt gaaatttgcc
    14461 tttagaagca ccgatattaa cgaaataaaa cagacccttg aatttttcag cattgacaaa
    14521 gaggacgaga acaaccagaa gcgattgcgt gatttggaaa acgggcaatg cttgcttcaa
    14581 gatttatacg gacgtgtggg cgtggtgcag atacacccag tctttgaaga attactgcac
    14641 gcctttgata ccagaccgcc gatacagcga aatgaggtgg agtgatgaaa gaaaggataa
    14701 aaagtgtgtt cacaagaaaa aagatattcc gctttctcaa aatggctctg tttgtcgtgg
    14761 cactttccct tatcctgctt tcacttttgg gaacggtggc acacgcaacg ggacttgtgg
    14821 acgacactat caacaccgag aacctttatt ccaaatatcc gctgtcgaat tatcagcttg
    14881 atttctatgc agataataac tgggcgtggt tgccgtggaa ctggctggac gggattggaa
    14941 aaagtgtgca atatgggctt tactgtatta ccaactttgt ctggacgata agactatatc
    15001 taagcaatgc aacgggctat gtagtgcagg aagcctacaa gctggacttt atcaacgata
    15061 tggcagacag tatcggaaga agcatacaga cccttgcagg cgtaactcaa aatggttttt
    15121 ctacttcggg cttttatgtg ggatttcttc tgctgattat cttagtcgta ggtatctatg
    15181 ttgcctatac gggactgata aaacgggaaa ccagtaaggc actacacgcc gttatcaact
    15241 ttgttgtggt gttcgtatta tcggcttctt ttatcgccta tgctcctgac tacatcaaga
    15301 aagtgaatga cttttcatca gacatcagta ccgcttcact ggacttggga acaaaaatca
    15361 tgcttcccga cagcgacagc aagggcaagg acagcgtgga cttgataagg gatagcctat
    15421 tttccataca agtacaacag ccgtggctac tgcttcaatt tggtaattcg ggtatagagg
    15481 aaatcggggc agaccgtgtc gaagctctcg tatcggcaag cccagaggac gaggacggca
    15541 agaccagaga ggaagtcgtg aaaacagaaa tcgaggacaa cgacaacaac aatctgacga
    15601 tactgcaggt ggtaaaccgt ttaggtatgg tgttcttcct cttgttcttc aacttgggta
    15661 tcacgatatt tgtattcttg cttacgggta tgatgttgtt cagccagata ctttttatca
    15721 ttttcgctat gtttctgcct atcagctttc tattaagtat gataccgagc tatgagagca
    15781 tggcaaaaca agccatagtc cgtgtgttta atacgattat gacaagggca ggtattacac
    15841 tgattgtcac ggtagccttt agtatttcca gtatgttcta caacatatcg acggactacc
    15901 cgttctttat ggtggcgttc ttgcagatag tgtattttgc aggtatctac atgaagctgg
    15961 gcgatttaat gagcatgttc tctttaaacg ctggcgacag tcaaagcatg ggacggcgaa
    16021 tattccgcag accgtatctg ttcatgcgac atagggctag acgtatggaa cataggcttg
    16081 caagagctgt tagtgctggt ggtgtcgctg gtgcggtggc tggtggtgtc gttgccagtt
    16141 caaaatctac acgccagaat ggaacaaaga acaatcgtgg aaatacaagc tccagcatgg
    16201 ggcagaaagc aggttcagcc gtgggagctg tccttgatac aaagaataag gtcaaggaca
    16261 aggcaactgc tgtcaaagaa aatatcaaag atatgccgac acagactgcc tatgcggttc
    16321 actctgctaa ggaaaaggcg aagtccagtg tgtctgattt caagcgtggc gtggtgcagg
    16381 aaaaggaaaa ccgccagtct ggacgcttaa agaagcaaca gctacataga cagaatatcg
    16441 ctgacaagcg tatggaactt caaaaggcac aagaatcaag gcaggcaggt aagaaaacgg
    16501 acggatcagc gacaagcgga gctacccgtc cccatgaaag accagctacc acttctaagg
    16561 ttaatgtaga aaaacagcag gaagtcaaac gccctgccat agcttcaacc gtgaagaatg
    16621 atgaacctat caagaaaaat gttgtcaagg aacgtccact atcttctggc gatacatcag
    16681 caaagaccgt caatcaagaa accacaagac agaattacaa gactgaccgt gttacgaaaa
    16741 caaccgttca gaagcataca cagcagaata ccgagaagaa caaacgaacc gtattcaaga
    16801 aaggacaaaa gaagaaatga aactaagaca tatcgctatt gttggcagtc tgttttctat
    16861 cctcttttct ctcgtattat tctttggggt tctgattagt gccgaggacg acgacggggg
    16921 caatggttat tcctctaatt atgtaggtat gaacctatct gccgaagtct tgaaacatca
    16981 gcctatggta gaaaagtatg ccaaagaaaa cggtatttcc gagtatgtca atgtgttgct
    17041 ggtaatcata caagtggaaa gtggcggtac agccgaggac gttatgcaaa gctctgaaag
    17101 tctgggacta ccacccaact cactagatac ggaaagctca atcaagcagg ggtgtaaata
    17161 ttttgcgtcc ctgctttctt cctgcaaaaa tcaaggtatc agtgatataa atgttgtgat
    17221 acagtcctat aactatggcg gtggctatgt tggctatgtg gcaggaaacg gaaaaaagca
    17281 tatgttcaac cttgcggaaa acttcgcaag ggataagtcg ggcggtcaaa aagtaaccta
    17341 tacgaaccct atcgccgtgg caaaaaatgg tggctggcgt tatggctatg gaaatatgtt
    17401 ctatgtggag cttgtgaacc agtaccttgt tgtcaatcaa gtatcgggcg agctggcaca
    17461 aaaggttatg aatgaagcct taaaatatca aggttggaag tatgtatatg gtggcagtaa
    17521 cccgaacact tcttttgatt gttcgggact ggtacagtgg tgctacaaca aagctggcgt
    17581 atctttaccg agaaccgcac aaaatcagta tgacgcaacc cagcatatcc cactatcgca
    17641 ggcaaaggca ggcgatttgg tctttttcca cagtacctac aatgcaggaa gctatgtaac
    17701 ccacgtcggt atctatgtag gaaacactca gatgtatcat gcaggcgacc ctatcggata
    17761 tgccgattta aacaatgctt attggcaaca gtacttgatt tgtgctggaa gaataaaaca
    17821 gtagaaagga ttggaaacca tgtttaaaaa gaaagaaaaa ccagtaaaag aaacgaaagt
    17881 ccgcactatg aaagtaggca cacataagaa atctgtgatt gctctgtggg tggtgcttat
    17941 cgccagcgta tcatttggcg tgtataagaa ctttaccgcc attgatatgc acacggtaca
    18001 tgaaacggaa accattcagt taagactaaa tgacaccaac ggagttgaaa attttgtgaa
    18061 gaacttctgt aagtcctact acacatggga caacagcaag gaagctatcg aagcaaggac
    18121 gcaggcaatc agcaactatc tgacaaagga gttgcaggac ttgaaccttg ataccattag
    18181 gacggatata ccgacaagct ccactgttac aaatgtactg atttgggata taaaacagtc
    18241 tggaacgaac gattttatcg ctacctacga agtagatcag cagataaaag agggagaaca
    18301 gacaagcaat gtcaaagcaa cctataccgt caaagtccat gtggataaag acggcgatat
    18361 ggttatcgta cagaacccaa ccattgcacc agcgattgaa aaatcagact atgagccaaa
    18421 ggcacaagaa gccgacgcaa gcgtggacgc agataccgtc aatgacgcta ccgcattttt
    18481 ggaaactttc tttaagctgt atccgacagc aacagaaaaa gaacttgcct actatgtggc
    18541 tggcaatgtg cttgaaccta tcggcaggga ctacctctat tccgaattag tcaatcctat
    18601 gtttacggat gacagggata atgtgaaagt cagggttgcg gtgaaattca ttgataatca
    18661 gacaaaggca atgcaggtat cacagtttga gcttgtgtta cataaggata gcaactggaa
    18721 aattattatg taagatattt tgttggcagg ctctattttc ggatatagtc tgccttttct
    18781 atagcaaatg tgctgcacta acaagcgatt tcattgtatt ttaagcaatt aaatctttct
    18841 tgacaatttt atcttaaatc atatatttat ggatattgac tttctgcttg caataagtta
    18901 taatatgact ataaaactaa attagtcata taggaggcgt gtggtatgta taaagaagtt
    18961 ggtatttctt caaataatgt tccgataaca ctttctattt ggtatacaga aagaaatagt
    19021 cctaccattg tttttttgcc gggtactatg tcacaccctc tcatgtatga gaattttcta
    19081 tgtggtttga gtgaaagagg atttaatata ataggcattc actatctttc acacggaaaa
    19141 agccctaaaa tcaaaaaaaa ttatacaatg gaagatttgc tgcataatgt atatgacgca
    19201 acgacttacg gaatagaaaa ttttggtgaa aacatagctg tcatgggttc cagtcaaggt
    19261 gggattttag ctgcaatggc ggcgggtagg gacacaaggt taaaagccgt ttttccacat
    19321 aatatcatgc tgactacatt aaaagaaact atgagcttaa caaaataccc cgcatggtca
    19381 caccgtttta tggggctgat acagttgggc tttcgtgtag gtggaaaatt gctgcctaac
    19441 tataaggttc caggagacgc ttatttagac tatgacagag tattttatag tgaacaggca
    19501 aaacaagatt gcttaaatga ccctatgctt ttaccctatt atcctttaag atttgtcgcc
    19561 agcttattta atgctgactt atcgtgcttg gagaatgggg ctatacaatg tcctgttatt
    19621 ttaattactg ccaaaggcga tccgcttttt agatttgatt ataccaacaa agtatttgat
    19681 ttaattcatg taccgaaaaa ggaattactt actttagagc ttaatcgtca catgattttt
    19741 aatgaggaca cggacactac aataaacgca ttggaagcta ccttgcataa atatcttgga
    19801 tagtatattg attatataat cagtaaatgt tatacttttc atgacgataa aaacatttta
    19861 gtcataaggg ggttatgagt ttgccacgat ttacggaaca agaaaaagaa atcataaaca
    19921 gtaaactttt gatagaggga gaaaagctgt tttcattata cggcttaaaa aaagtaacag
    19981 ttgatgattt agcagctgct gtaaacatat caaaaggttc attctatgct ttttatccca
    20041 gtaaagaaca tctatatgtg gaaatcaatt ttcgcttaca aaaagagtta tttacaagta
    20101 tagaaacaac tgttaaaaag aaaaagtata agaaccatag agatttagca aaggacgtta
    20161 ttatgctaag tttaacgggg gtgattactt cgcctattct ttcacagatt gatttatctt
    20221 taatggacta tctgcaaaga aaaatatcgt cagatatttt tgaaaatcat atgtacagtg
    20281 atattcgtat attggagata ctggaaggtt taggggttgt atttttggtt ccgcatacgg
    20341 ttatcataaa atccctgtat tctgttttat cctgtttgga acaattcaaa gaggacgaag
    20401 aattgaatat ggtacaaaat cttttagtga atggtattat tcagcaagta gtagcagagt
    20461 aataattttg tttggttatt tgacaggcaa gtggccaaac aaatttttta cagtttatat
    20521 gactatatat ctataaacgt cataaagtta aaggaggaaa gaataatgat tgatgtaaaa
    20581 aaactgtatt ttagttatac tgacaaaccg tttgttgaga atgtaagttt ccatgttggg
    20641 agaggagaaa ttttcggttt tttaggaccc tctggtgctg gaaaatcaac tattcaaaaa
    20701 attttaactg ggcttaatac aagttataaa ggcagcgtaa aagttgcagg tacagaaata
    20761 agggagcata caaaccggtt ttatgaaaat attggagtag actttgaatt ttcaacctgc
    20821 tacgagaaat tcacagcacg acaaaatctc gcctattttg cttctctata tgacaagcaa
    20881 cctcggtcta ttgatgaatt attgcatatg gtaggcttag aaaatgatgg ggacaaaaaa
    20941 gttgctgact tttccaaagg tatgcgttct cgcttgaatt tcattaaagc gttagtacat
    21001 gaccctgata tattattcct tgatgaacct acaagcggtc ttgaccctac aaataatcgt
    21061 ttaatgaaag atattattct tgcggaaaag aaacgcggaa aaacgattat cattactact
    21121 cacaatatgt atgacgcaac agagctatgt gaccaagtag cctttattgt tgccggaaaa
    21181 gtcagtgcat tggatagtcc acataatttg attatgtcaa ggggagctgc caaaatacac
    21241 tatacctact atgataaagg cgagaaaacc ggagaatgtc tgcttgatag aaccacagaa
    21301 gataagttgt taaaaagttt aatttccgaa aaccgtctgc tttctattca tagtagtgag
    21361 cctacattaa atgatatttt tgttgatatt acagggagga cacttcaatg agattgggac
    21421 gtttaatttg tggtgatata catttccaat ggaaatatgg tttctatttt atctacttta
    21481 tattaacagt tttatatgtg tgtgggattg ccgctttgac gggacattgg aaaacggata
    21541 ttgcttctat catgatatac tctgacccgg cagctatggg attgtttttt atgggggcga
    21601 ttgtcctttt agaaaaaagc caaaaggtat taaatgctat ggtagtatcg cctgtaaaag
    21661 tatcggagta tatactttca aaaaccatcg cattgattgc tatttctaca atcattgcgg
    21721 tgatattggg atttgtttcc ggcagcaatc atttacttgg tatcgcagtt ggtacagctt
    21781 tgacatcagc aatctttact atgcttggca ttatcgcggc tacaaaaatt tcaaacttga
    21841 accaatttct cattgtgatt atgccgatag aaattgtttg ctttgttcca cctatcgttg
    21901 gtctgtttgt aaagttacca gatatattcc gcttttttcc gttcacagct tgcatgaacc
    21961 tcataactgg aaaaagcatt ttactatcgt ttgatatggt acttgtaatt gctacgttga
    22021 ttatcctcta catagttgcg cgacatacag ttaagcatat gtggagaagt ttgggaggtg
    22081 taaagctatg aaaaggatat tatccagtac catacaggta tttaagcaaa tcaagagcga
    22141 ccctatgatg tttgcagctt gttttacgcc ttttgtcatg ggagctttga ttaaattcgg
    22201 tattcccttt cttgatggaa taacagattt ttctttgcaa acatactatc cgatctttga
    22261 tttattactt tctatcatgg ctcctgtatt gctctgcttt gcatttgcaa tgattacatt
    22321 agaggaaatc gacgataaag tgtcacggta cttttcgatt acccctcttg gtaagtcggg
    22381 gtatctcttt acaaggttgg gagtaccctc aattatttcg gcggtcatcg cttttattgt
    22441 actgttgctt ttttcattag aaaagttgtc cattggaatg acaatatgtt tggcgcttct
    22501 cggctcagta caggcaatta ttgtttcgct tatgattata acattatcaa gcaataaatt
    22561 agagggtatg gcagttacaa aactagctgc acttacatta ctcggaatac cagctccctt
    22621 ttttattgat agttactacc agttcgcggt tggcttcctt ccatcatttt gggtagcgaa
    22681 agccatgcag aatgaagcgg ttctttattt ctccatagga ttgatggtag ctttggtctg
    22741 gtactacttc cttataaaac gtctgtttag gaagctggca ggataagaag gcatattatg
    22801 gaaaaaggta gacttctgca aaacgaaaat ataaataagg ctattcaggt ttccaaaatc
    22861 acactaatca tcaacctggg tttatcgcta cttaaatttg ctgccggata tataggaaag
    22921 tcaagtgcca tgctgtcgga cgctgtacac tcggcttctg atgtattaag cacaatcgtt
    22981 gtgatggttg gaattaaaat atcagaaaaa cagcctgata aagaacatcc ttatgggcat
    23041 gaacgtatgg aatgtgtggc ttccattatt ctctctgttg cattggctat aacgggtgct
    23101 ggaataggat attcaggtat caagaaaatc ttttcaggac agtacaatac gctttctgtc
    23161 tctagtggaa tcgcacttac ggcggcagtc ttatccattg ttataaaaga gtggatgtac
    23221 tggtatacaa gaagtgctgc aaagcataca aattctgacg cacttatggc tgacgcatgg
    23281 catcaccgct ctgatgcact ctcgtcggtt ggttccttaa ttggcatttt aggagcacgt
    23341 ttaggctatg ctattctcga ccctattgca agcattgtga tttgcggttg tatcttaaaa
    23401 gctgctttgg acattttcaa agaaagcatt aacaaaatgg tagaccatag ttgtgacaat
    23461 gctactgaaa cgaagattag ggaagttgtg cttcagcagc aaggggttga tggtattgat
    23521 gaattaaaaa cccgcatgtt tggagcgaaa atgtatgtag atattgaaat attagctgat
    23581 ggtaatttag ctctttatga cgctcataga attgctgagg gagtccatca aacgattgaa
    23641 aacaactttc cacaatgtaa acattgtatg gtacatgtta atacgaatga acttccctat
    23701 tctccatagg acagggaaga tgatttaaga ctattgaaag ggaaaggagg ggaagtaggt
    23761 tatggaacaa tactggtgga ttatcattgt tatggttttg attgtcgttg gcttacttaa
    23821 aattgtaatt atgaagaagt ataaccaaag gagaaattct aacaatcgtt caaaaaatag
    23881 cgaggatgac aagatgtatt aagagcaatt atccgatggt atgagatata tacctatgaa
    23941 atttgaacga taaatcccag tttgtaaaag tcaataaatc caaagataaa tttgtatttg
    24001 aaaggagttt tgaagaatga aaaaatatga gtatgtagaa attaattata atgcgaaagg
    24061 agttgtattc ctttgtactg ataaacacaa agaaattatt gacagttatg caaaaacggg
    24121 gtatcactac gcagggtttg tacccacaga aattgacgca aaaggctgta tgcgaaagat
    24181 agatttaatt tttgaaaaag aagattaact tattttaggg agttggttct aatgatagga
    24241 cttaaaagac gagatatgaa ttatattatg aaagttggtg ctaaagctca aggtgtttct
    24301 gtatctgaat ttagggctat gctacaagat accatagaca aaactatgga aagtacagac
    24361 ccagacgtgc aggcaaattt taaacgatac tttggaaata agcggccaac accagaggaa
    24421 tatatttata cgattaccaa aaagacaaag gttaaggttt aaatgttgta aagggggctt
    24481 gccgtatgaa acgctttaaa tacaattcag tatattcaag cccaatttaa cagcatacta
    24541 tttacaatta aatacgctat accagtagct cgtacaaaat ttatattttg tgcgggcttt
    24601 tttaataccc gaaaagtttt ttaattattt tcattttttt ggaagcaaat cacaccttgc
    24661 tgtacagata tggtgcggaa agagggataa acacttttca attcatagct ggaaaggagg
    24721 tgagattatg aaaccgtctg acttccaaaa aacaattcag tgtcagtttg aatgtaagct
    24781 gaaaaaagtt gtcaaaggta ttatctggaa ctaccgcaaa gaattaagac gaagaaaaaa
    24841 caaggaaata tcttttagcg aattaccaga cattacggta gaaagtctgg caacatggga
    24901 cgactacgaa acaaactaca caatttttaa tgtgtgtggc atagatatta aagtccttga
    24961 tgatgaactt gcagaagctc tcaaacagct acccgaaaaa aacgtaacaa tgtattgatg
    25021 tattacttct tggaaatgag tgatacagag attgcagata ttatgaatta taccagagag
    25081 gctgtgttca aacacagaca ccgtgcacta gaaactatga aagagatact aaatgaaaag
    25141 acaagacagt aaaggacgca atttgcgtct tggagagagc cagagaaaag acggaagata
    25201 cgtttataaa tataccgata tattcgggaa gccacagttt gtatattctt ggaagcttgt
    25261 acccacagac aagacacctg ctggaaaacg tgatgatata tcgttgaggg aaaaagaaaa
    25321 gcagataaaa aaagaccttg acgacggtat cgacacggtg ggcggtaaaa tgacagtctg
    25381 ccagctttac gaaaaaaaga acaatcaaag aaagaacatc aagagaaaca cggaattagg
    25441 acgacagtat cttgtgaacg cactggaaaa tgacccattg ggtatgaggg cgattgatac
    25501 ggtcaagcag tccgacgcta aagaatgggc tatcagaatg aatggcaaag gttattctta
    25561 caagacgata gataactata agcgttccct aaaggcttca ttctatatgg cgatacaaga
    25621 cgactgtatc agaaaaaatc cttttaattc caagttgagt gatgttctgg aagatgatac
    25681 ggaagcaaag gttatcctga caccagagca ggaagaaaaa ctccttgcct ttatggaaac
    25741 agataatgtt tacagcaagt atcgtgatga agttattctt ctgctggaaa cgggacttcg
    25801 tatctctgaa ttatgcggac tgacaacaca cattgatatg cttaaccgtg tgattaacat
    25861 agaccaccag ctattgcgtg atacagaggt gggctactat atttccaaag ctaagacaaa
    25921 aaacggtaag cgagagcttc cattaacaga aagagcatat caagcactca agcgaatttt
    25981 aaagaaccga ggaaaagcac aaccgcttgt tgttggtggt tatagcaatt tcttattctt
    26041 gaaccgtgag ggattgccaa aggtagcagg aaattatgaa agtatggtgc gtgggttgat
    26101 taagaagtac aacaagaccc ataaggacaa gttaccaaac atcacgcccc attcattcag
    26161 acatacttac tgtacgaaca tggcaaataa gggaatgaac ccaaacaccc tgcaatacat
    26221 catgggacac gcaaacataa ccatgacatt agggtattat gctcacggta ctttccagtc
    26281 tgcaaaggca gagttagaaa ggctggcttc ttaatatcga aaatgtatct tactacttat
    26341 ttactacttt tggttgcatt ttcatgctgg taaatgccag cttatgcaag gtatcttcca
    26401 aaaagaaaat gcccatacag cccgaaaatg cgggatatat cggcatctac caacttatga
    26461 aaagataatc agaaatttag taagttttta taataaaaaa ataagaggct gtccaaaaat
    26521 agagataagc cttttttcat aatcggaatt ataaaaatac acttgatttc atatgaaaaa
    26581 gagtgtctca taaactaaaa agtttacttt gagacactac cttttttatt cttatttata
    26641 ctttgtagac atattataat atgcgtttca ctggtatctg aacttctgtc aaccagtctt
    26701 cttcactctc tttattccat ataccatcta tataagattc tctaggattt ccaaccactt
    26761 tatatttatt gtcctctatc catttaaatg catctgcata agtttttcct aatgttgaat
    26821 atggaccatt gtgtaatata caaactgcat tttcaactgc atctaattct ttaaatttta
    26881 atatatccgt atcttccttt aaatcattta tagcttgaca catttcaaca tccatatcag
    26941 tctctctata ttcaccatca tgatatatat taaaacaata atcaggaact gcacatgtac
    27001 accctgtttt catcatttct tcagccatta catttggaaa tagattaaaa agttcatcat
    27061 atccctttaa cacttttctc atagaagcaa ctttaacttt tggtaaagac ttaataatta
    27121 catcattcat attgacttct cctttcaaat tcataaggta attacgtagt ttaattattt
    27181 tatattcttc acttttaatt acatccaaca tttctttttc tttattcatt aaaatatttt
    27241 caacttcttc aggattttct acagcttttt ttatctcagc taatgataaa cccatttgtt
    27301 ttaaagttat tatctgatgt aatttaggta attgctctgt tgtataatac ctatattttg
    27361 taaattcatc tacatattct ggttttaaca accctatatc ttcataatac cttaatgtct
    27421 ttgtagttat tttatttatt ttagaaaaca ttcctataga aaacatctca tcacctgcgc
    27481 attagatttg attaatttaa gtttaaactt tccccttagg ttaaagtcaa tacaaaataa
    27541 attataattg tattaattct tcaattctat ctccactcaa taccaaaata tcccctgttt
    27601 ttataatggt tgaaccttta ggtattaaaa cctgttcctt tctttttatc ataactatta
    27661 atatatcgtc tggtatatta gaatctatta tactcttatt agcccattta ttaccctcat
    27721 caacatttac ttcgataagt ttagagctaa tttccccatc ataatcatta aatgttttta
    27781 atacaggtgt attatcatca actaaatcca actttctggc tacagttggt atcaatgaac
    27841 cttgtattcc tactgaaagc aacgctataa aaaatattat atggaatatg tcattctcta
    27901 tggctactcc ttgagtaaca gcataaattg caaaaactac tgaagccgca cctctaagac
    27961 ctacccacga tatgaataat ttttctttat ttgagaattt aaattttgct agaactataa
    28021 atgttgctaa tggtcttgca ataactatca tgaatataga tatggccatt gctataccaa
    28081 atacatttgg taacttagaa gggaaagata acaatcctaa cataaagaat aacattattt
    28141 gcataatcca tgaaattcca tcaaagaaat gaaccaaact ttttttaaga ggtatcctac
    28201 tatttcctat aataatacct gaaatgtaaa cacttaaata tccatttcct ccaagatact
    28261 cgctaaatgc ataagaaagt attgcaatag ctgttataaa tatagtataa aatccttcta
    28321 tctcgaagtt tgaatgcctt aaaatataaa cagataattt tgctagtata aatcctatag
    28381 ctaacccaaa agctatttgt tttattacca ttggtgcaat aagttctacc ccagaattat
    28441 tcatcaatgt caaaagaatt agtgtcaaca tatacgctat tgggtcatta cttccacttt
    28501 caacttctaa taatgatgca agtgaacccc ttaagtttag tttttgtgaa cgcaacacag
    28561 caaacacaga tgcagcatct gttgaagctg caattgcccc aattaataaa ccttcaagta
    28621 aactagttcc tattaccatg tggcaaaaca tacctgtaac cccagacgta attattacac
    28681 ccagtgaaga cattaatata gaaggtatag caacaggttt agccatattc cagtttgttc
    28741 caaaccctcc aaaaaacatt ataaatatta gtccaataga acataattgt tttgttaatt
    28801 catagttctg aacctcccat gactaaagtc acagagttcc tgcttcatag aattttgcat
    28861 actaaaatat gtcttactaa ttctccacag gctatcccca tagttcctac ggttcttaca
    28921 taagttattt attttgaatt gttattagtc ttaatccttc ttttaatata tttatacttg
    28981 catttatatc tctatcgtga gtttcattac aactaggaca aatccattct ctta
//
LOCUS       DAEPOZ010000023        28253 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.023, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000023 DAEPOZ010000000
VERSION     DAEPOZ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28253
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.023"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(138..1388)
                     /locus_tag="KR440_000834"
     CDS             complement(138..1388)
                     /locus_tag="KR440_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HBH0697897.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLYEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDSDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1420..2361)
                     /locus_tag="KR440_000835"
     CDS             complement(1420..2361)
                     /locus_tag="KR440_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBH0697898.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
     gene            complement(2601..3677)
                     /locus_tag="KR440_000836"
     CDS             complement(2601..3677)
                     /locus_tag="KR440_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBH0697899.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
     gene            complement(4050..4565)
                     /locus_tag="KR440_000837"
     CDS             complement(4050..4565)
                     /locus_tag="KR440_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HBH0697900.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(4928..5998)
                     /gene="leuB"
                     /locus_tag="KR440_000838"
     CDS             complement(4928..5998)
                     /gene="leuB"
                     /locus_tag="KR440_000838"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBH0697901.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDEIGEPLPNKTVETCRKNEAVLLGAVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTRAMGELIVNEI"
     gene            complement(6005..6496)
                     /gene="leuD"
                     /locus_tag="KR440_000839"
     CDS             complement(6005..6496)
                     /gene="leuD"
                     /locus_tag="KR440_000839"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBH0697902.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKEKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(6511..7788)
                     /gene="leuC"
                     /locus_tag="KR440_000840"
     CDS             complement(6511..7788)
                     /gene="leuC"
                     /locus_tag="KR440_000840"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBH0697903.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTITKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(7745..9487)
                     /gene="leuA"
                     /locus_tag="KR440_000841"
     CDS             complement(7745..9487)
                     /gene="leuA"
                     /locus_tag="KR440_000841"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBH0697904.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIKGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(9611..9857)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(10005..10451)
                     /locus_tag="KR440_000842"
     CDS             complement(10005..10451)
                     /locus_tag="KR440_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697905.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTDESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(10472..11191)
                     /locus_tag="KR440_000843"
     CDS             complement(10472..11191)
                     /locus_tag="KR440_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBH0697906.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYSGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGTRVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(11309..12583)
                     /locus_tag="KR440_000844"
     CDS             complement(11309..12583)
                     /locus_tag="KR440_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBH0697907.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
     gene            complement(12783..14150)
                     /locus_tag="KR440_000845"
     CDS             complement(12783..14150)
                     /locus_tag="KR440_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0697908.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIRYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
     gene            complement(14400..14864)
                     /locus_tag="KR440_000846"
     CDS             complement(14400..14864)
                     /locus_tag="KR440_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HBH0697909.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            complement(14869..15498)
                     /locus_tag="KR440_000847"
     CDS             complement(14869..15498)
                     /locus_tag="KR440_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBH0697910.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADKVIGTEFFYENSRYKNQFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            complement(15532..16419)
                     /locus_tag="KR440_000848"
     CDS             complement(15532..16419)
                     /locus_tag="KR440_000848"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HBH0697911.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYSLYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            complement(16448..17686)
                     /locus_tag="KR440_000849"
     CDS             complement(16448..17686)
                     /locus_tag="KR440_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697912.1"
                     /translation="MDGDILMSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYNL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNVNGFIEILNIGIKSYPV"
     gene            complement(18070..19212)
                     /locus_tag="KR440_000850"
     CDS             complement(18070..19212)
                     /locus_tag="KR440_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBH0697913.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
     gene            complement(19353..20219)
                     /locus_tag="KR440_000851"
     CDS             complement(19353..20219)
                     /locus_tag="KR440_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBH0697914.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            complement(20553..22100)
                     /locus_tag="KR440_000852"
     CDS             complement(20553..22100)
                     /locus_tag="KR440_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBH0697915.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVVVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            complement(22093..23229)
                     /locus_tag="KR440_000853"
     CDS             complement(22093..23229)
                     /locus_tag="KR440_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0697916.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            complement(23485..24582)
                     /gene="dinB"
                     /locus_tag="KR440_000854"
     CDS             complement(23485..24582)
                     /gene="dinB"
                     /locus_tag="KR440_000854"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBH0697917.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGKPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKSSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVEVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            complement(24972..26477)
                     /locus_tag="KR440_000855"
     CDS             complement(24972..26477)
                     /locus_tag="KR440_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBH0697918.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            complement(26739..27089)
                     /locus_tag="KR440_000856"
     CDS             complement(26739..27089)
                     /locus_tag="KR440_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697919.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKTVTSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
     gene            complement(27312..27503)
                     /locus_tag="KR440_000857"
     CDS             complement(27312..27503)
                     /locus_tag="KR440_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697920.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            complement(27622..28170)
                     /locus_tag="KR440_000858"
     CDS             complement(27622..28170)
                     /locus_tag="KR440_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0697921.1"
                     /translation="MGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDSLKKADIEPVL
                     LKDNMNIVINSAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGDAEEERLSEFI
                     NGNTLKYDFVKMPHHGRYDKLTETFLESTSPQYAVITCSEKKEPEEGVLKILERLNIK
                     TFLTSSGEVVINSDGKTLSVNQ"
BASE COUNT         9169 a         5115 c         2938 g        11031 t
ORIGIN      
        1 cctaaaatta tccatttcta aaaaaaggta actattatta agaatatcaa cttatagaat
       61 ttatattcca catacgattt tcaatcaaaa aaacaagggc taaaaagtga attgtacact
      121 ttttagccct ttattaatta ttcatttatt tcatgtaaga atagcccaca tcttacttta
      181 ggttcaaacc atgttgactt agctggcatt atcttatttg catctgctac tcttattaaa
      241 tcttcaatac tagtaggata cattgcaaat gctactttca tatcttcatt aactcttttt
      301 tctaattctt caagacctct aattccaccg ataaaatcaa ttcttttatc tactctagga
      361 ttgtcaattc ctaaaatagc atcaataaca tagtcttgta atatagaaat gtctaatgag
      421 tcaacagggt cttctatttc taataaattt ttatttgctt tcattttata ccacttatca
      481 cctaggtaca ttccgaaagt tccttttttg tcaggtttat atttttcttt tccttctatt
      541 tcttctagtt caaaattttc ttttatttta tttataaatt cttcctcgct taaaccattt
      601 aagtctttga taaccctatt ataatccaaa accattaaat tctcatctgg tgcaatcatt
      661 gctatgaagt aattaaattc ttctttgcca gtataatttg gatttttagc tctcatttct
      721 ttggctacat tttctgctga agctgtccta tgatgaccat ctgcaatgta tagataatct
      781 aatttttcaa atttatctac taggttatct attatatcat cactatcaat cttccatact
      841 gtatgctcta tcttgtcatc tgttatgaaa tcatataaag gttcattatt atccataaaa
      901 tcattgatta tatcatctat caccctttga tgtttgtaag ttacaagtat agttcccgta
      961 ttagcatcac agtattttat gtgtttagtt ctatcaattt ccttttctgg tcgagtgtac
     1021 tcatgttttt ttattgttcc attcaagctt tcatcaactg atatacatgc tactatacca
     1081 acttgagctc taccatccat gatttgttta tatatatata tacactcttt gtcatctttt
     1141 actagaattt cttgttttcg aaactcatat aaattttctc gcgcttttaa ataaactttt
     1201 tcatcatata aatctatatt ttcatctaaa tcaatttctg atttatctat atgcaaaaat
     1261 gaatatggat tcccttctgc aattcttcta gcttctttac tattcataac atcgtaagga
     1321 agtgctgcta ctttatctac tatatattta tttggtctaa tcgccttaaa tggtttaatc
     1381 gttgccatta ctaatttcct cctaattata caaaatattt tataatgcta cccctattag
     1441 attgttgaag tcaagaaaga aatcttgaat cacatttact atttcgtctc ctattctttc
     1501 ttgagcttct actgtagatg ccccaatatg aggtgtagga gaaactcttg gatgatttac
     1561 taagtctaaa tttaatgtag gttcttgttc ataaacatct atagcagctg ctgataattt
     1621 accttcatct aatgctttta gtaaatcttt ttcatcaact aaaccgcctc ttgcacagtt
     1681 tattaaataa gcccctttct tcatcttatt tatttctttt tcagttatta ctgccccctt
     1741 attcttatca aatggtatgt gtaaagataa gaaatctgca ttttctaaaa cttcttccat
     1801 atcacagaat ttataattgt taaaaccttg cgcctcaccc attatgtcag tatatataac
     1861 attcatacct aataattctg ctctctttgc aacttctttt gcaattcttc caaaacctat
     1921 aagtccaagt gtctttccat taatttctgt acctttatac ttcttcttct cccattttcc
     1981 atctctcata gttacatttg cagtatttat gaatcttgca agaacaaata attgacctat
     2041 tgtaagctct gcaacagata tactgcttgc atttggagta ttcataactt ttatgccatt
     2101 agcttgagca tatttaacat ctatattatc cagacctact ccacctctta ctatcaactt
     2161 caacttttta ccctctaatg ctttatctat tacatcttta gtaactttag tagctgaacg
     2221 tactacaagt acatcaacat ctttaagttt atctcctaaa acatcttttt cataaaactg
     2281 ctctatcaca ttaaaatcta actctctcaa ttttctcgct gcttctttct ctattccatc
     2341 agtaactaat atattataca taacaaactc ctccaatttt tattcactta atttttccaa
     2401 tttttatttt ttaaagtatt ttcatcaata aaaaacttta cttaatttaa ctttctgttt
     2461 atataatttt caaaatattt ttttattaaa gtcatatcaa attttcctat tgtttgttaa
     2521 aaatttaata tgtaattttt ataaaatatt atttattata tattctaaat ttatcttaaa
     2581 acatacatat ttttacaata ttatttatta aaacctaata tatcatcaat attatctaat
     2641 aattcttgta catcttcgac agtatagtca cccatatgtg aaattctaaa agttttttct
     2701 tttaaatctc catagccatt tgctatttgg aatcctcgtt cttgtaattt tgaatttaaa
     2761 tttgatacac taataccttg agtattttct ataacagtaa gtgtattgga taagtgattt
     2821 tcattggtaa aaatttggaa gtgtttttta gcccagtttc taactaagtt tgccatgtct
     2881 tcatgtctgt tgaatctgtt atctaggcct tcatctaata tattatctaa ttggaaatct
     2941 aaagcgaaca tgtgagaaag agatggtgtt gaagggtatt ggtagttttt cttgattaag
     3001 tattcataca ttgctaacaa atcaagatat actcctctaa aaggtacttg cttagctcta
     3061 tcgattgctt tttgagaaaa tgtgcaaata gacattcctg gtggtaatcc cagtgctttt
     3121 tgtactgatg ttatacaaat atctattcca atcttatcaa cctcgacttt tattccaccg
     3181 gctgaactta cagtatctac acagtagatt acatcttcat actttttaac aacttctcca
     3241 atttcttcta ttggatttct tattccagtt gaagtttcat tgtgagttac agttattaaa
     3301 tcgtattctc cggtagaaag caccttatca accatttctg gcgttgttgc ctctcctaat
     3361 tcaactttaa atatatccgc tgggacatta ttagccactg ccattttgta ccatctatca
     3421 ccaaaggatc cacaggaaaa tactgctgcc tttttggatg tacaggaacg aatggagcct
     3481 tccattaaac cagttccgga tgacgtggat aaaagtatcg tgttgtttgt atagaatagt
     3541 ttttgcatct tttcacttat actcttttgg agcatagatg catcttttcc tctatgtcct
     3601 atcattggtg tagccatctt ttgcaagact tcttctttaa catcaatagg accaggaata
     3661 aataatttct tactcattat aattcccccc tttttagttt gtaatatgga tagcgttttt
     3721 actgtcctta tttttaataa ctataatatc aagctgaatt aactatgtca acataaaatt
     3781 tattttttat atataattta ttttatatat tttatgaaaa cattcatttt ctgccttttc
     3841 ataagtctga attttctgaa ttattctctc aaaaaaaata tactttttat ctatataaat
     3901 taattttttt agtatgtaaa tttgacgcta ttacgataaa tctttttagt ataactaatt
     3961 tatttttatc ttgtattttt attgtatttt tgaattttat tttttctaaa tataaaattt
     4021 atgttttaaa atataaaatt tttatttaat caaagttcaa tatattccac tttttctttc
     4081 gattcttcaa aaaaccattc ttcattagaa aaatcacctt ctgcaatttg tccaataggc
     4141 attttcatat actttccagt tgctgtcact gctatatctc catttggtag aattatttct
     4201 cctgtacctt caaataattt tctgttttct ttagttattc ttccaacaac ttttatagta
     4261 gcatctgttg gtactggttt cttatatttc aactctaaag atactgttac tccccatatt
     4321 tggtcatcat ttatacttac agctcttcca atagtctcat ctaatattgc agcagacata
     4381 cctccatgaa ctctcccagg ataactttga tgccaatcct ttgtattaca aattgataca
     4441 agctctccat tttcaagttc ataaaaatct gctttaagtc ctagctcatt ttgtgttcca
     4501 caaactaaac aagatttact actattttgc ttcttcaaaa ctttatactt tgccaaaaaa
     4561 tccatctcct aaccgacatt agtagtcgct taatattaaa taattctaaa atatattata
     4621 taatattaat aaaatccctg caaacttcta ataataaaaa taggctactt caatatgata
     4681 agtttaattt aaagtattat tttccaaaaa ataactttat caaaatagaa actcaaagta
     4741 gatataagcc tctttttcgc tattaaaata gtaaaaatat atttgactta atataaaaaa
     4801 gagtgtctca ttaactaata aagtttattt tgagacaccc ctcataaaac aaattatact
     4861 acaatagatt tttactaatt ctacaagtta tatatttaaa ttatattatt caaattaaac
     4921 tataattcta aatttcattt actattaatt cgcccattgc tcttgttcct acaacatttc
     4981 ccacaccatt atagatatca atagttctat aacctttatt gagtacactt tgtacagcat
     5041 cttcaataca cttagcttct tcttctaagt taaaactatg tcttaacatc atgcttacag
     5101 atagaatagt tgcaattgga tttacaatat cttttccagc tatatcaggc gcacttccat
     5161 gtattggttc atacatacca aaactatctt ctctaagtga tgctgatggt aacataccta
     5221 tagaacccgt aatcatagat gcttcatcag atattatatc tccaaatata ttattagtca
     5281 ctattacatc aaattggcta gggtctttta caacttgcat agcagcatta tcaacataca
     5341 tatgtgacag ttctacatcc tcaaactctt tagctaagtc ttccattata cttctccaaa
     5401 gtcttgaact ctcaagcaca tttgccttat ctacacttat aagtttttta tttctatttc
     5461 tggctatttc aaaagctctc ttaccaattc ttcttatttc attttcgtta tatatttcta
     5521 catcataagc attttcgata ccatcaatga tttttcttcc acgttctcca aaataaatgc
     5581 cccctgttaa ttctcttaca acaacaaaat caatgccttt ctcaactata tccgttctaa
     5641 gtggtgaagc ttcttttata ctttcataca ttgtagctgg tcttaaattt gcaaataaac
     5701 ccaatgactc tcttaatttt aaaagaccag actctggtct tttatctccc ttacatttat
     5761 cccactttgg tcctccaaca gcaccaagca atacagcttc gttttttcga caagtttcaa
     5821 cagttttatt aggtagaggt tctccaattt catcaatcgc gcatcctcca gctaatacat
     5881 attcacaatc gaatctatga ttaaatttac aacaaatttt atttagaacc tttatacctt
     5941 catctataat ttccggtcca acaccatcac cttttatgac tgctatatta caattcatac
     6001 tccactaccc cctatttgct tttattgagt taattaagcc ttcatttaat ataatatttt
     6061 gcataaactc aggaaatggt tctgctttat attctttttc ttttgttata ttttttatca
     6121 cacctgtaga aaaatctact tctatattat ccccttcatc tatgttattg gcagcttctt
     6181 cacattcaag tataggaagc cctatattta tagagtttct aaaaaatatt ctagcaaaag
     6241 tagaagcaat cacacaggat actccacttt cttttattac aattggtgca tgttctcttg
     6301 atgaaccaca accaaagttt ttagttgcaa ctataatatc tccttttttt accttagaaa
     6361 taaatttatc atcaatatcc tccatacaat gcgttgctag ttctttataa tcagctatat
     6421 ttaaatatct agctggtatt attacatctg tatctatatt gtcaccgaat ttaaaaacac
     6481 ttccattggc aatcataaat tttacctccc ttaaattatt tctgatggtt ttgatatttt
     6541 tccagttata gctgatgctg ctgctacagc tgggcttgca agatacacct ctgaatcaac
     6601 atgccccatt cttcctacga aattcctatt tgttgttgaa atggattttt ctcctgctgc
     6661 aagtataccc atatgtcccc ctagacatgg accacaagtt ggtgtagaaa ctgctgctcc
     6721 tgcctctata aaagtggtaa tatacccttt ttctatagct tcaagatata ctttttgagt
     6781 tccaggaaat atcataactc taactccatc tgctactttt ttgcctttca ttatctcagc
     6841 tactacttca aggtctgata ttcttccatt tgtacaagaa cctattacaa cttggtctat
     6901 ctttatatcc ttatctatct catctactgt tctagtattt tcaggaagat gaggaaaggc
     6961 tactgtttct tttagttcgg ctaaatctat ttcgtatact tcatcataga ctgcatcttc
     7021 atctgcttca tatatattta catcttttgt tatagttgaa cacttatgtg aatttatata
     7081 ttccatagtc ttatcgtcta ctggaaagat tccatttttt gctcctgctt ctattgccat
     7141 attacaaata gtaaatctat catccataga taagtattct actccatctc cacaaaattc
     7201 catcgacttg taaagtgctc catcaacacc tatctcccct attatatgaa gtattatatc
     7261 tttaccactt atccatttat ttggtttatt cttcaaaaca aattttattg ctccaggaac
     7321 cttaaaccaa cattttcctg tagccatacc tgctcccata tcagttgaac ctacaccagt
     7381 agaaaatgct cctaatgctc catatgtgca agtatgagag tcagcaccta taattacatc
     7441 tccagcagtt actatacctt tttcaggaag aagaacatgc tctattccca tatctcctac
     7501 atcaaaaaaa tttacaactc cttttgattt tgcgaaactt cttgtaaatt tacattgttc
     7561 tgcactcttt atatctttat taggtgtaaa gtggtctaaa accatcacta ctttagtctt
     7621 atcataaaca tcttcaattc ctaatttttc aaattctcta atggctactg gtgatgttat
     7681 atcattacct aaaactacat ctaaatctgc ttcgattaat tgccctttct taacttcatt
     7741 taatttagca tgtttggcta atattttctg cgtcatagtc atccccattt tttatttcct
     7801 cctcaatata acttttattt attgcattca taagtgcatt aatagctgct gtatttatgt
     7861 tttccgatat acttacacca aaatagactt cattattgtg ctctattcct acataagcta
     7921 cccctcttga atgtgaccct tcctctaatg catgttccca gtaatatcta aatttataat
     7981 ctttcatgcc acattgcttt agtgcattat taaatgaatc tacaggtcca tttcctatac
     8041 caacaatcct ttgttcattt cccatataac ttattcttgc agtcatctca atcatgttag
     8101 tatcattaga atcatcattt tcatagttta attcatccat tgattttatt ttatacttct
     8161 taactttata agggctctca acttcaatat attctttctt aaataaatta aatattgctt
     8221 cactactaag ttcttcccca attctatcag attccatttt gactacattt ccaaaatcag
     8281 aatgcatatt cttaggtatc ataaatccat aatctgtttc cattatataa acagctcctc
     8341 cttttccaga ttggctattt atccttataa tttctttgta ttctcttcca atatcatgag
     8401 ggtctattgg taaatatgga acttcccagt aatcattatc tctgtttttc attgctatca
     8461 tgccttttct aatagcatct tgatgtgaac ctgaaaaagc acaatgtaca agattacctg
     8521 catatggatg tctatcatgt accataagtt ttgtacagtc ttcatatata ccaattatct
     8581 tatctatatt gctaaaatct agctctgggt ctattccttg tgtataaaga ttaagtccca
     8641 catttactat atccatattt ccagttcttt caccatttcc aaatagtgtt ccttcaagtc
     8701 tatcggcacc agccaataat ccaagttcac ttgcagccgt acaagttcct ctatcattgt
     8761 gagtatgcaa acttagtata atagaatccc tacaacttat atttttacaa aaccattcta
     8821 tttggtctgc atatacgttt ggtgttgcca tttccactgt tgatggaaga tttattatta
     8881 cttttttttg aggagttggt ttccaaacat ctatcactgc ctcacatatt tcaagtgcat
     8941 aatccatctc tgttcctgta aaactctctg gtgaatattc aaaagtaaat tctgtttctg
     9001 gatatttttt tacttcttca ttaaataatt ttgctccttc aactgctata tctattattt
     9061 cttgtttact catattaaaa accacatctc tttgaagaac agatgtagag ttatataaat
     9121 gtataattgc ttttttacat ccctttattg attcaaatgt tttttctata agatgagccc
     9181 tagattgtgt aagtacttgt atagttacat catctggaat catattctca tctattaatt
     9241 ttcttaaaaa tgtatactct gtatttgatg ctgatggaaa tcctacctct atttctttaa
     9301 atcctgtatc aataagcatt ttgaacattc tgactttttc atttacactc ataggcgttg
     9361 gcagtgactg atttccgtct cttaaatcga cactacacca aattggagcc ttaaatattt
     9421 cattatctgg ccatttacga tttactattt gcattttatc atatttttta tactttccac
     9481 acttcatgtt atttccccct ttttctgttt tttgtattca gtatacctta tatcgacgca
     9541 atcctgcaca cttcataagg ataaatgtca attttatcat tagaataaaa aaaacctcat
     9601 ctctaataaa attagagacg aggttttgct cgcggtacca ctctatttag ctttctaaaa
     9661 agctccctta gtaatagcca atacaataat tataatgact atatttccta taacgtggaa
     9721 ttactccgat tattctaaga tttaaatctt agaaaacagc tcctagacaa gttcagaact
     9781 atcaactact aaatcacacc atcctttagc tctctgaaag tttcattgtt cttactactt
     9841 ctattcatca cttttttaat catttaaatt ttctaaatat tttgatttaa tagttattat
     9901 atacacatta aaatgtattg tcaacaaaaa aaataattat ctcaattttt tatttttgat
     9961 aagcaattct ataaaacatg ctaactattg aaaacagtat gatattatgc cttaaacttt
    10021 ttcatctttt catctatgat attcaataat tttttttcat cttccaaact caatgccctt
    10081 aatctaattt tataattaga ttcgtcagtg tatatattta atatattttt tactacttct
    10141 attttttcta ttatcttata attaaacttt tgactttgtt tagattttat actgaatata
    10201 cattgattat ttattaatat tgaatgatta tcgagagtaa tatatacttt tccctcatat
    10261 ttatttgaaa aatcacgacc ttgtttaaat agaaaaactg ctgttattat acataaaata
    10321 tataatattg tcgttgaata tataaaaatt gtagctaaaa ttataagtgc taaaaccata
    10381 tatatagtca ttcctttttt aaaataacca ttgtcataat aattcatata gataaactta
    10441 tttttactca aattttttca ctcccaagat tctaaaaatt tatatcaaaa aattctataa
    10501 tttcatcttc atgataattt atgactaatt catctggaaa atctactgct tctaaaactt
    10561 tttctgcatt ttcaaattct cctatagaat aacatatatg agagtcagta ccaagaatta
    10621 ctcttgtacc atatgtttta cacaatgtca acatatgact tacattttcc caagtacccg
    10681 ttctatgaga gtttgaactt aatgatgaat tatttatttc taaaaggata tttttatctt
    10741 tagctttttt tactataggt tcataatcta aaggatacct actgtcatct ggatgtccaa
    10801 ttatttttac cttaggttta tccataactt ttaaaatagc atttgtattt tcttcttttg
    10861 taccagaagc tatacaaggt ctatgcaaac ttgcgattat ataatccaaa tgttgcaaca
    10921 tatcttcttg aacatctaaa ttaccactat aatccattat atttccttct attcccctaa
    10981 gaatctttac accttgcact tcacgtggaa gcaaatgcaa attataaaaa tagaatggat
    11041 gtggtcctcc tggcatatta ggtccgtggt ctgatattcc taagtatttt atattcttct
    11101 tactcgcctc ttcgacattt tccttgaaag tactatgtgc atgtccactt attagagtgt
    11161 gacaatgtac atccataata gactttttca ttaaatttcc tcctaatatt ttacataaat
    11221 tcgtgaaaat agctaaaggg ctatttcaaa actaaagctg tcataataga cagtgtaacg
    11281 ttttaaaaca gccccatttt caattatttt acacttctaa atctttaaat ttatctttaa
    11341 ataattctcc aatacttcct aaagattcct ctacttctag gtattctgtt atatcctctt
    11401 ctggctctct agttgcttct tttattgaaa ccaacattct tttagtcttt ggtttaaaat
    11461 caagaatctt aactttaacc tcatctccaa tattaacaac attagtaact ttagccactc
    11521 tagtatcact caattcagat attggtaaat acgcatctac accttcttta atttgaacaa
    11581 aagccccttt ttctattatt ctaagaactt tagcattgac aacatcatca atttgtactt
    11641 cttttgctat taaatcccaa gggtcattat ttatgtcttt taatgccaag gcaatttttt
    11701 tagtctcttt atttacatcc aacacataaa cttctacttc ttgtccctct gaaacaacat
    11761 cttcaacact ttcaactctt gtccaagcta aattatttat atgtgctaat ccttcaatcc
    11821 caccaatatc cacaaatgca ccataaggca ttatctttgt caccttacca tctctttttt
    11881 gtccaacttc aagagaatta aataagtctt ctttagctgc tttgatacga gctttttctt
    11941 cttctctttc ttgcttaatt cttgctcttt cagcttcttt ttctgcctta atttgagctc
    12001 ttctttcttc tctctcttta tttatacgct cttgtaatac atctctatga gatgctacaa
    12061 gtctattttt tctaacatcc aattctttta tataaacttc taagttaaga ccaacaaatt
    12121 tagatgtatc tgtaataaat tttgtatcaa gttgagaaat tggtataaaa aatgtatatg
    12181 tattgtaatt agcaaataca cctttatcta tattcttttc aacatttaca gttattattc
    12241 tatgttcatc ataagccttt tgtaactcgg caaaatcatt tcttttgtca agttgtaatt
    12301 tagataaagt tattgtacca tctttttgag atacttttgt tattacagca gttatttcat
    12361 cacctatgtg gtatatatct tctatatatt gatttttttc tatgttcaat tcacttatag
    12421 gtattattcc atcaaaccca tagtctaatc ctaattgtac ttcatcattt ctcactgcag
    12481 ttatttttcc agttgtcaat tctccaactt tcactttgct gaatacttgt tcttgctggt
    12541 caagtaattc ttgcattgtt aaatcatttt ccattataaa catcctttcc tcttattgat
    12601 tatcatatct gtaaatatta taccattaat attgcaaata cttacatttt tcttactata
    12661 atttttaaaa taaattcaac ttcatatatt tatacattat agaaaatatc ttattaaatt
    12721 aaaatacaaa ttctaatttt tatctaataa aatattttca taaaaaattt atattgtata
    12781 tcttattctt ttattgcttt aggttttgat agaaaacatt tataaaatat aaagaaagaa
    12841 attgctgtta tagtagcagc tgttatatct gcaactggct ctgctaaaat tactgcaaaa
    12901 agtttttcat taaagaatgt tgggagtata aatattaaag gtattaaaag tacaatcttt
    12961 ctaagtagag ctaaaaccaa agatattgtt gcttttccaa gtgctaaaaa tgtctgttga
    13021 caagctattt gaatcccaaa cataaaagca ccagcaaaat atatttttat actccaacta
    13081 gtcatactaa gtaactgcgg attcttatta aatatactaa caaaaaatgc aggaaatatc
    13141 attaataaaa gccataaaat agtagaatat gtaaaacatg acaataaaca caacttaaat
    13201 gtctttttta ctctatcaag ttgacctgaa ccataattat aacttattat tggttgtgct
    13261 ccttgtgtaa cccccatatt aggaagcata agaatttgca ttatactgct cattatggcc
    13321 attgaaccta ctgctaaatc tcctccatac atttgtaatt tactatttaa ggctataagt
    13381 actaaacttt ctgtagattg cattataaat ggagagattc ccaagccaag cactggtaag
    13441 atgatacttg cttttggtat catatacttc ttttttattt taagtatact tttctttcca
    13501 aagagaaaca taagtaccca aatagcagaa acgccttgtg caattatagt agctaatgca
    13561 gctcctttta cacccatatt aaagccaaat ataaaaatcg ggtcaagtat tatgtttatt
    13621 aaagcaccta tagcaactgt catcattcca gttgtagcaa acccttgggt atttataaaa
    13681 gtattcatac ccattgaaat ctgaacaaac actgtaccta ttaaataaat atttaaatag
    13741 tccattgaat atctaattgt agattcactt gctccaaaca tccaaagtaa aggctctttt
    13801 ccaatcttaa aacctatagt caaaagtatt gctaaaatca ccagtacaga aaaactgtta
    13861 ctcattattt tctctgcctc atcattgttt tgctcaccca tttttatagc agctaatgga
    13921 cctcctccca taccgattaa ataactacat gcagttatta taattattat tggaaaggcc
    13981 actccaacac cagccatagc aacttctcca ttttccattc taccaataaa aatcctatcg
    14041 actatgttgt ataaaacatt tacaatttgt gcaataatag caggtgtagc aagtttaaat
    14101 aataatttac ccacactttc actgcctaag ttagtgtttg aattttccat tttaaaacct
    14161 cctcaatcca ataattaaaa tgtatcacta aaattttatt ctatataaaa atttcatttt
    14221 atcagcagac aaatttagag tactttccct acaagtaata ctatactata tttcttttgc
    14281 catagtaaac attttagaac taactgaatt taaatacttt atctcatttt ttatttatta
    14341 ttaaaaattt ttcacatgta ctcataaaaa aactatccaa gtatattact tagatagttt
    14401 tatattatat gtaaagaaag agctgaaact ataacccctg ataacaaatt gccaagaaat
    14461 actcccataa aagcatcttt tattctcatt tttaatatgg aagctatagc agatgcagtc
    14521 catgttcctg tagttggcag tggtatacct acaaatagta ttataccaat tatcgaaaca
    14581 ctttttaatt tcttcattct tgagtttatt tttctatcaa ttacatcagc aatcccttta
    14641 aatagtttct tccctcttaa aaattgtaat acatgtctga atgtcaatat caagggaatc
    14701 gataccaatg tagaacctat tacactaaat acataaaccc atataggatt taagcccatt
    14761 gcaataccta taggaatggc tcccctcaat tctgttacag gaaccataga taaaattaat
    14821 atgtgtaaat atcccaaact attccctcct attctaagtg acattaagtt atgaatttat
    14881 ttgctctata acctcaccat cctttttact aactaaaaat gcatttttag tcaatgaaag
    14941 cattggtaaa tcactcttag agtctgaata tgcatatgaa ttttcataat caatctctat
    15001 cccaagttta cctaatacag cctttattct ttttactttt tcaattccct tacagttttc
    15061 tccaatgaat tgatttttat atcttgaatt ttcataaaaa aactccgtgc ctataacctt
    15121 gtctgcaaga ccataatact taaaatattt catatatgca tatggagatg cagttatcat
    15181 aattataaca ttatcttctt ctttattttt gtaaatgacc tttttcatag attcgttaat
    15241 ttttttcatt atgcaatcat caaaaaactt ttttaaatct tcttcactaa aatctactat
    15301 tggataaaag attgcctctt ttgccttctt caagggtatc attctaatta aatacataaa
    15361 tagaataaaa ataatgtatg gcaactttat aatagtttta aaggttttat tttttaaaga
    15421 gtaaataaaa aaatctatca tgctatcagt atttataaca gtataatcta catcaagtaa
    15481 taatacactt tttttcataa gtatttcccc atcctatcac taaaaatatc actaaaaaat
    15541 caatattatt aaacatgtaa ttatccataa gattacgttt atttttaaag cattatcttt
    15601 taggacaata tcagtaggat tattttcatt tgtagtgtta attatataat tatatctaag
    15661 tacaccataa actacaaata ttgttgtaaa tatcatatgt ggcttttctg gattacttgt
    15721 agaatacaat gaataacata caattgtaga acttaatata actatcatca tttgatttag
    15781 attttctatg tcataatctt ccaatatttt tctatgttca actgcatcat ctcttagagt
    15841 ttctatttct tttttacgtt ttccaagagc taagtacaga gataagaaaa atgtacataa
    15901 aattatccaa tttgataaac taacacttat ggctacacat cctgcataaa ccctaagtat
    15961 aaaaccaaga gatattgaaa acacatctaa tagtattata ttctttagtt taagagaata
    16021 taatatattg tttatcaaat agagactaag aattatagaa agattaaaac taacaactgt
    16081 tgataaacag actgaaatta aagctaatac tacaaatagt atattagcac tttttttgct
    16141 tattcttcca cttgccactg gtctattaca ttttttagga tgttttttat ctttatccat
    16201 gtctatagca tcattcagtg catatactga tgatgacatt aggcaaaaac acacaaacgc
    16261 aataaacgta tttaaaaata aatttatatt tagtatgttg tttgaaaaaa ttatagctga
    16321 aaatacaaag aaattcttta tatattgctt tattctcatt aactttatat aatctgaaat
    16381 tttagtaaga agccttatac ttgtattggt ctgtttcata aacttactac cctttcaata
    16441 ttatttttta aacaggatat gattttatcc ctatgtttaa aatttctata aatccattga
    16501 cattactgat tactatagta accaataaca gcgatagaaa tatattatac atattctttt
    16561 cattttttct aagcgctttc gtaaagtaag ccaataaaat tataactgca aaactaaaat
    16621 ttatagccat aatacttgaa ttgactagat tatatcctac tataccatgt aaagttagat
    16681 tgaatataat tattcccata catagcttag gaactctttt ttctgaattt tcaataactg
    16741 ataaaacaca aaaacataaa gctaataatg taagaattac tggtattgta gcaacttctc
    16801 cattttgcaa aaataaccct tttttaatac ttgctccagg aaaataaaaa accgaataca
    16861 ataatctctc tataatcatt gcaatctttt ccaaaattgg ttctccactt ataaatttcg
    16921 tatcatactg tattacattt ccaaggttca taaaactctc ataagacttc gttgaaacta
    16981 cacaaatata tgccattagg ataaatgtaa ttaaatgttt taacattttt cctattttta
    17041 atttaaactc aaatatcata ttaaaaaaat acaaatatat attactaata gttattccaa
    17101 atagaggaat agctgctaat tctactaaat aagaattttt tcctttcatc ttatttgcaa
    17161 taactatgaa aaatattaat gaaaattggc tatatataaa tctctctaca tttaaagtca
    17221 taaataactg tggaaaagaa aatatcatta tcatagtaag taaaattaaa gttaatctct
    17281 gcatttttat ctttttaata tgattaaaga ctaaagttat tgacatagca ctagttatta
    17341 tttgtagaca aattagtcca aacccatagg cactattaaa atctgttgtc ccaggatttg
    17401 ctattgaagt acaaaatagg tatattggat atgcaaaaat tgatataatt gctgaaaaca
    17461 atatgtgctt actattgtct tgacttatag caaatacatt taaattgaat aaaacccctg
    17521 tatctgtatc taacaataca tcataagtat tgtttaaatt ataaaatata gtagtcttag
    17581 aaaaaaatat cttagttatt attaagtaag ttattacaac taatataaac aaaaacatct
    17641 cgtttttata ttttaatatt ttactcatca atatatcacc atccattcat tatataggat
    17701 ttgctgacac ctacaattta ttccttatat ttaatagcca tcttcataat gttcttactt
    17761 gttttattgt aattgaataa ctctttattt acaaccacaa tatttacttt attgatgtta
    17821 taatcagtca agtattgttt atataacata gattagtgtt gtataaaatg agaatttgta
    17881 agcaatatta atgtaaaata tttgaaaaac tttatataaa aatatacaat gttgcttatg
    17941 gaattaaact ataacaaatt aaagcataat ttaaggagac aaaattataa tctgtctcct
    18001 taaatattaa aactatattt taaaacatta ctaaatacta tacaaatttc aaatatagta
    18061 taaaattaat tagactttaa cactattagc tccatcttct tcccattttt taatttcttt
    18121 taatctatct attataggta aatgctgagt acattcaact tcacattttc cacaagatat
    18181 acattcttta gcttttgcct tactattcat tgtgtaattc caatgtccta aaccataaac
    18241 taactttgtc atttcttcat cactcttttt aaacatctgc ttttcattat aaaaaagcat
    18301 ataggctggg gtattaatac cttttggaca tactttacaa taaccacaac ctgtacatat
    18361 ttcgttcata ttcttattta atctagtctc aatatcatgt atatcctcat ctgaataaat
    18421 tctattttcg tctgcaattt tacatgcatc atctatatct tgttttttat taaaccctat
    18481 caaagatata cttatttgtg aaattcctat gttgaatctt aaagctgatt ctatagtact
    18541 ttcgccatct tttgatagaa aagaaagttc tttattatac tttggaatag ttccaccact
    18601 taatggattc attgccacaa caccatatcc attatcaaca gcatatttgg caccttttag
    18661 cctatatgga aagtttaata tatttattcc catagttaca ccttcaaact tattttcact
    18721 tagaatatct ataacctcat ctcctggttg atgagatgaa aatactatat ggtctatcaa
    18781 tccttcttct ttacatttca ataatccttc atattgacct ccaggtctca ttgctagttc
    18841 gtaatgttcc atctttctaa tattccaaac atgataaaaa tttattttag atacgcctaa
    18901 tcgttctatt gattttttta cagcctctat agctttttct gatgtatcac atgcttttgg
    18961 cttttcttta gttgatacat aaaaatcggt cttaccttcc tttatcattt gtctaaaggc
    19021 tagtccaaat atatcttcac ttctgtcatc acaataccct ggcgctgtat caaagtaatt
    19081 aatacctttt tcatacgcat atttgactaa atcagcattt tcttgattac ttttttctaa
    19141 atcaaacctt aatcctccat agccaactac tgaaaccatt ttccctgtct ttccatattc
    19201 aacgtatctc attgtaaatt cctccttaga tatattaaga cttaatttct ttatagaaaa
    19261 tattatacca taaataattt ttataaatta tttatggtat aatatttttt ttataaatta
    19321 tattttttta taacttgtat tttatagcta agttaaaaac tataaaaagg ttcaatatgg
    19381 actattactt gagcattttc attaatttct tcttgtattt gtttctcaat tttatgtgtt
    19441 aaatcatgag attgctctac gcttataaat gggtctacca ttacatgcat gtcaatataa
    19501 atagaacttt tgctacctct acttctgata ttgtgaacat cccttacctc attaaattca
    19561 aacacaattt cttttatata atcctcatct acagccactc tatcgaccaa aattccaata
    19621 gatggcctaa atattccata agccgaataa attatgaatg cagaaataat aataggaact
    19681 atagattcta ttattactgg aaatcctaac ttaacaccaa ctaaagtaac caatacacct
    19741 agagacacaa atatatcact tcttgtatgc aatgaatccg aaattaaaac ataactattt
    19801 agtttagtcc caattctata ttcatacatg catacaaaaa tatttataaa aagtgtaata
    19861 attaaagcaa ttaaactttc tatagttata gcaggtacaa ctggattagc tattcttagc
    19921 actgcactaa gaattatctt aattgctata acaaacaaca taccacttat aaataaactg
    19981 gttataattt caaacttttt atgtccataa ggatgatttt tatcttttgg tttagatgca
    20041 aagaaaatac ctataagccc cactatatta gatgctccat ctgacaaaga atgaaatccg
    20101 tctgctgtca tactatagct ttttatttga ttaccaataa tgatttttaa taatgctact
    20161 gcaaaattag caaataaaat tatccacaat acttgcttca ctttcttata gttatccata
    20221 acaaattccc cctcccttcg attcaataaa gaaagctatc tatcaatatc aatcacccag
    20281 ataataaaat cgtatataag cacatttact atatttatat tcaaaattgg ttgtacttgt
    20341 tcattctatt tgaaacttaa ttataaataa aaatcgtagg tatggttttg gtatacataa
    20401 aaataagaga ctgtctcaaa ataaagataa gcttcttttt tattgttgaa atgataaaat
    20461 atatttgatt tcatataaaa aagagtgcct catgaactaa aaagttcatt ttgagacact
    20521 cccttaaatt tttgatataa actattaatt ttttatacta agccttcttt aactaagaat
    20581 gaatgagcaa cttcttctgg tgatttacca agttcatcca cttgatagtt aagttctctc
    20641 atagtttctt catttatttt tcctgaaagc atattcataa ctttttctaa ctcaggatat
    20701 ttcttcaaag tatcttctct tactaatggt gctgcataat atggtacaaa gaaattttta
    20761 tcgtcctcta atatcttaag attaaacttt ttaataagac catcagttaa aaatgcatct
    20821 ataacctgag attcattatt atctaaagct gtatatctta aacttccatt cattgctttt
    20881 acatctttga attttaaacc ataatctcta gaaagcccta caagaccatc ttctctattt
    20941 tcaaactcca aagttggtga gaatgtaaat tctttaccat atttagtcaa atcagaaata
    21001 gtatttattc catacttttc agcagtttca ggcatcatag ctaatgtgta agtattatta
    21061 aatccaagag ggtcaagtaa agttaactga ttttcttttt ccatagtatc ttttacaaca
    21121 ttatatgctt catcagcatc tttaatcgga gcatgtttca ttacatttac caaaagtgta
    21181 cctgtatagt caacatacat gtctaactca ccagatttaa ttgcattaaa cgcaacagat
    21241 gaaccaccta gatttaaatt cttcttaact ttaagattcg tatttttttc tactaattca
    21301 gcataaacat taccaagtat aagttgttct gtataatctt tagaaccaat agttattgta
    21361 tcttttttac ttgaaaaact agaactaaaa acaacgaaca acattgaaac aacaaccact
    21421 acagatatta ctttaagtgc tatataattt ttcttaggtg cttttggatt taatcctttt
    21481 ggagtaactg caacttcaat tttactaaat aaataatcaa ctaacagtgc aattatacat
    21541 gatgggattg cccctgctaa aatcatatta ttatttacag tttgaacccc tgaaaatact
    21601 aaatatccta aaccaccagc acctataaat gctgcaagag tcatcaaacc aacagctgta
    21661 actgcagata ttctaactcc acccattatt ataggaagtg ctaaagggaa ttgtatttta
    21721 aataatgtct gatttcttgt aagaccaata cctttagctg attctaatac tactgggtca
    21781 atacttgtta tacctatata cgtatttttt acaattggta gtaatgaata aaccactacc
    21841 ataaatatgg ctggtttact tcctatgcca agtataggta caagaagacc caaaagtgcc
    21901 atagacggaa ctgcttggac taagtttaca aatccaagta caaactttct aagagatgag
    21961 attcttgaaa taattattcc aagaggaaca ccaactatta tagctattaa aatagatgta
    22021 acagttagac tcatatgttg tattaaaagc tctataatct tatctttttg taatagtata
    22081 aagttaaaaa aattagccaa gaacactcac ctccatttct aagaattgtt cacttaaaac
    22141 agaaagtaaa ctacttctag taataagccc taccagttta ttttcatcag aaataaccgg
    22201 aatatatcca accgaattac gattcattac atttagtatt tctactaaat tatcaccttc
    22261 atttacatgc aaaggctcac tcgacataat atcagcaagt aatattgact tatcatcaat
    22321 atctttcata tcttttacag taacaatacc ttttaatgtt tttgctctat ctataattag
    22381 taagctatca accttactcg ttctcataat ttctattcct tgagttacag ttctagtgga
    22441 attaacagca ataggatttt ttatcataat atctcttgct tttatatatt ctggattatc
    22501 ccaaactcta tcttctccga taaaatcttt tacaaagtca tttgcaggct ttctaagtat
    22561 attttcggga gtatcatatt gtgctattct tccatccttc ataatacaaa ttttatcagc
    22621 tatctttaaa gcctcatcca tatcatgcgt tacaaatatt atagtttttt tcaattcatc
    22681 ttgaagagaa aataattgtt cttgtaaaga tgttctcgtt attgggtcta aagcactaaa
    22741 aggctcatcc atcaatatta tttcagcatc agtagcaatg gctcttgcaa caccaattct
    22801 ttgttgctgt ccaccactaa gttctgaagg atacttattc aaaaattcat cactatcaag
    22861 tcctaccatc tcaagtagtt gcaacgttct tttttcaatt tcttcaacgg atttttcctt
    22921 ttttaggcgt ggaattagct ctatattttc ttttattgta agatgaggga ataggcctgt
    22981 gttctgtata acatatccta tttttcttct aagctctatt tcattttcct gagaaattgg
    23041 tttgccatta atatatatct caccagatgt aggttttatt aatttgttta taagcttcaa
    23101 agtagtagtc tttccacaac cacttgaacc aatcaaaact accaatgtac ctgtttctac
    23161 aactagagaa atatcatcta atataacatt atttcctatt ttttttgtta catttcttat
    23221 ctctatcata atttatcatc ccctttttcg aacaagcaac cttctccaat attataagtt
    23281 gtttttataa ttatatcata ctattttatt tatgtaaaca aagtacgatt tttaggtcac
    23341 tatttgtctg aatattgcta caaatataaa gtatttatat atagtatata atgtcaaaaa
    23401 acaaaaagta taaaaatatt cataaaataa cactagcctc aattatatag tattaaagct
    23461 agtgttattt catttaaaca aaactcacat ttgaatacta tccatgtatt caaataatgt
    23521 aatatcttta taagtttctt ttttatcact taagtttgat attgtaactc ccaatagcct
    23581 aatttgtttt ccattaacct caacatttct caacaaatta tctacaccac ttcttatatc
    23641 attatattcg tcaatatagt gttctaaact aaggcttcta gttatctttg taaagtcctc
    23701 atatttaatt tttaatgtta atgtttttcc aaacttctca gagcttttta atctatgaca
    23761 tacttcttca caaagttcat caagaatgtt gcgtgtttct tcttcatcaa tatccaaatt
    23821 atgacttaat gtagtctcag ccccaacaga ttttctaacc ctatttggtt ccactggtct
    23881 atcgtctatt cctctagcaa atttatataa ttcataacct ctttttttga atatattttc
    23941 aagttcaaat aaatttaaac accttaaatc atatccagtt tttataccta aatttttaag
    24001 agtatttgaa gtaacttttc caacaccaaa aaatttattc acaggtaatt tatctaaaaa
    24061 gtcctgggca ttttcttcgg ttattacagt taacccattt ggctttctta agtctgatgc
    24121 aatcttagca agaaacttat tgtatgacac accagctgaa gaagtaagac caacttctct
    24181 aaatatatct ttttttattt gctttgctat ctcaattgat gaatcaatat tttttttatt
    24241 ttttgttaca tctaagtaag cttcatctaa agataatggt tcaactaaat ccgtgtatct
    24301 ataaaatata tctcttattt gctctgacac actcttataa acatcaaatc taggtcttac
    24361 aaaaactgca tatggacacc tattgtaagc tacagctgat ggcatagctg aatggatacc
    24421 aaattttcta gcttcataag aacacgttgc aaccacacct cggctttgtg gatttccacc
    24481 gacaataaca ggttttcctt ttaatttttt attatctctt tgttctatag atgcataaaa
    24541 agcgtccata tctatatgaa ttatttttct ataatgtttc attattatca ccttttaaag
    24601 tataaataga ttaattttat ttatattact ttttaattat accaaactta tgtttctttt
    24661 tggtattttt tcattatttt ctttatatat tcatattttc cataaataaa accatctata
    24721 atccattaac ttcaatataa tttataaaaa atccttaaaa acacttgcaa aaactatata
    24781 aaagctttac aataggtatt ccaaggaact aaatttaaaa ttccttatgg ataacagtat
    24841 atatatttaa tttatgggat gttttaaaaa caaaagactg tcttatacaa gacagcctac
    24901 tgattttatt ataattgtaa tggactgctc aaactattac tttaaaacag cccatatttt
    24961 tttatataat tttaactata gtaaagattt tgtgaaggat attctgggtc acaagttaca
    25021 tctataccaa gctttctaaa tgtttgttca tcatctctat ttaatatagt agtagaatga
    25081 gcttgacacc cttttaataa tgctaacttt tccatagcaa actgtgcagt tggattagtt
    25141 acagcacaaa tactaagtgc agttaacacc tcttggcaac taagtgaaac atttctacta
    25201 gctaaagtct ttgtttttaa gtttatgata ggttctaata ttacaggtga tattaagtgc
    25261 atatcatcag atatattggc taaatgttta atggcattta atactactgc tgctgttcca
    25321 ttcattaaat cagaaccctt accagttaat attgtaccat cttctaattc taatgcaact
    25381 acaggacaag tatcattctt gtcggcactt tcttttaatt tattactgta atttctagca
    25441 ggtaatacaa catttctatc ttctggcttt aagttaagct cttccataat cattttcatt
    25501 ctatcataag cgcctttatc aacttgtccc tttttatact cacaggcagt tttaaagtat
    25561 cttcttatta tttcttctat agaagctttt cttattactt catcatctat tattccaaag
    25621 ccaactctat taacacccat atcagttgga gattggaaaa ttgattcttt tccagttatc
    25681 ttttctatta tttttttaag cactggaaat aattccaaat ctctattgta gtttactgac
    25741 atttcaccat acgtttcaag gtggaatgaa tctatcatat tcacatcttt taaatcaact
    25801 gtagctgctt cataagctat attcaaagga tgttttacag gtacattcca aactgggaat
    25861 gtctcaaact tagaatatcc aactacatta cctcttttat tttcatgata caactgactt
    25921 agacaagtac caagttttcc actattaggt cctggtgctg tgactactac tattggcttt
    25981 gtagtttcta tatattcatt tgctccatat ccctcatcac taactatagt ctcaacatct
    26041 gacggatatc cctttgttgg ataatgctta taaactttaa ttcctctacg ttctaattta
    26101 tttataaata cagttgtagc aggttgtcct tcaaatctag taattactac actatttaca
    26161 tctagttcat aacctcttaa atcatctatt aatcttaaaa cttccatatc ataagtgata
    26221 ccaaagtctc ctcttatttt gtttctttct atatctcctg catagacaca aattacaact
    26281 tcaacttttt ctttcatatg ttgtaatact tttatctttg cattttcatc aaatcctgga
    26341 agtactcttt ttgcatgaag gtctcccatc aattttcctc caaactctaa ataaagtttg
    26401 tcaaaattat ttactctttc taagatgtat ttagactgct cttctaaata ttttttgtgg
    26461 tcaaatccta ctttcatctt aacacctcac taaattaaat ttcttgttga taacagtata
    26521 tttatatata tttttttagt aaaacatctt ataaaaaaat gttaatactt tttcactcaa
    26581 agtaatatta tacaattaag attaattttt ttcaagatag ttttatggta aataagatat
    26641 acctaatata actaataaaa taagaatatt atatttttat atataagtat aatattctca
    26701 ccttaacatt aatttatttc aatattttca tatatctttt attctaaatt caatactttt
    26761 ctaatatctt ctataccttg ataaacttct tgtttagaaa aattcatagc ttctaattct
    26821 tcatcagtct tttccataag catagtataa aattctattg ctatatactg catattttca
    26881 ctagttacag tcttagcttt atcaaataaa atttcttctg cttcgtttac atttccctga
    26941 aaaactaatc tttttaaagt tataagtata acttcttctt ctgtattata cactaaatct
    27001 ttttcttcat attcactgta tttttttccc aataaaagtc ttgcaaccat aagttttatc
    27061 tcattgaaaa tcttatccac tatagccatt tttacatctc ctataaataa attttatttt
    27121 ctttttaatt atatcagact ttattgtaaa aattattata tgtaaatgaa ataattcaaa
    27181 aataaattct aacaataaaa aaaagaaggt agcgggggat ttccgactac cttcaaacac
    27241 tagattgtga ttggtttggt attttattta tacccaccat aagctttatt aaatcatcta
    27301 aattgatttt tttagcatcc acatcctcca tcaaaaacgt caccacattc acaaagtatt
    27361 aataataata ttaagaaagg tatccatgca cataagtcca tttcagccca acaatcttgg
    27421 aatgctaaga ataagaagaa taatattatt atccaccaag cacatccacc aaatccgcct
    27481 tcaccaaaaa atctatccaa cataatatgt tacctcctag tcattttgta tgtggagttt
    27541 cagctcctaa aatatactat gtttaagggg taatatttgc taatgatttt tttaaatttt
    27601 ttttagatat tttaaaaaaa attattgatt cacactcaat gtcttaccat cactattgat
    27661 aactacctct ccactagaag tcaaaaatgt ttttatattc aatctttcca atatttttaa
    27721 aacaccttcc tctggttctt ttttctctga acatgtaata acagcatatt ggggtgaagt
    27781 agactctaaa aatgtttcag ttaatttgtc atatcttcca tgatgaggca ttttaacaaa
    27841 atcatatttt aaagtatttc catttataaa ctctgacaat ctttcttcct ctgcatctcc
    27901 tgcaaataaa aacttatgtg ctccatagct tatattagtg attattgaat aatcatttga
    27961 actctcgtac ttgctttttg aagccggatg tatgtttatt tctgcactat tgattacaat
    28021 attcatatta tcttttagta gcactggttc tatatcagct tttttaagtg agtcaatata
    28081 ttcatcatat tgcttactat ctttcttata atttgcttgt ataatattct ttactttaac
    28141 actagataac acactatcta ctccacctat atggtcctta tctaaatggg ttaaactgaa
    28201 cctcctatga ctaaagtcac agggttcctg cttcatagaa ttttgcatac taa
//
LOCUS       DAEPOZ010000024        27524 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.024, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000024 DAEPOZ010000000
VERSION     DAEPOZ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27524
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.024"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            19..1152
                     /locus_tag="KR440_000859"
     CDS             19..1152
                     /locus_tag="KR440_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HBH0697922.1"
                     /translation="MSNFILNGIFRTVIVSSLSILLILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTINILRSKKHININNINLEGFNNVSTISNNIILYTWIITVVVY
                     LIYTIFKYIKLKNLINDLSYDVDDREIVNLYKNILEEFNITKDIKLKYSYEVETPAFF
                     NSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLKTVYWFNPFIYIMDKHI
                     DLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKFIAGLHKQSDIKKRVMN
                     MFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNVLPKGFEDLRTVSSIRFSVIGF
                     TYADAPKNMREEYKINCESLGLIPKDSDKIEVGVEYYDSLLND"
     gene            2079..3101
                     /locus_tag="KR440_000860"
     CDS             2079..3101
                     /locus_tag="KR440_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBH0697923.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            3397..4317
                     /gene="pyrB"
                     /locus_tag="KR440_000861"
     CDS             3397..4317
                     /gene="pyrB"
                     /locus_tag="KR440_000861"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBH0697924.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            4356..5048
                     /locus_tag="KR440_000862"
     CDS             4356..5048
                     /locus_tag="KR440_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBH0697925.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDNIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            5041..5943
                     /locus_tag="KR440_000863"
     CDS             5041..5943
                     /locus_tag="KR440_000863"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBH0697926.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            6028..6612
                     /locus_tag="KR440_000864"
     CDS             6028..6612
                     /locus_tag="KR440_000864"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBH0697927.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            6827..7753
                     /locus_tag="KR440_000865"
     CDS             6827..7753
                     /locus_tag="KR440_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HBH0697928.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFNYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIGKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYILSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVENSLHRILEHSKEGRTSMLQDVEAHRPTEVDMF
                     SKNICKLGKKYSIPTPINQTLFYMIKVIESRF"
     gene            complement(7805..8695)
                     /locus_tag="KR440_000866"
     CDS             complement(7805..8695)
                     /locus_tag="KR440_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0697929.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSQKYPDIYVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYNSHLLKDFSFDKLQDQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            8883..9914
                     /locus_tag="KR440_000867"
     CDS             8883..9914
                     /locus_tag="KR440_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBH0697930.1"
                     /translation="MLKKKSSLTLKTIREILPGLFISVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGAIVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPKEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGTVQVVS
                     AVVLIKIFI"
     gene            10208..11083
                     /locus_tag="KR440_000868"
     CDS             10208..11083
                     /locus_tag="KR440_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine DNA glycosylase"
                     /protein_id="HBH0697931.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     RGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            11101..12591
                     /gene="cls"
                     /locus_tag="KR440_000869"
     CDS             11101..12591
                     /gene="cls"
                     /locus_tag="KR440_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBH0697932.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            12784..13068
                     /locus_tag="KR440_000870"
     CDS             12784..13068
                     /locus_tag="KR440_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBH0697933.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            13101..14729
                     /gene="groL"
                     /locus_tag="KR440_000871"
     CDS             13101..14729
                     /gene="groL"
                     /locus_tag="KR440_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBH0697934.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVIVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDAGMPGMGGGMPGMM"
     gene            14991..15731
                     /gene="larB"
                     /locus_tag="KR440_000872"
     CDS             14991..15731
                     /gene="larB"
                     /locus_tag="KR440_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HBH0697935.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            15815..16549
                     /gene="larC"
                     /locus_tag="KR440_000873"
     CDS             15815..16549
                     /gene="larC"
                     /locus_tag="KR440_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HBH0697936.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKSKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDELEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            16579..16998
                     /locus_tag="KR440_000874"
     CDS             16579..16998
                     /locus_tag="KR440_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HBH0697937.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKVSILCNES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGKLVKVTP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      17058..17159
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            17380..18915
                     /gene="guaA"
                     /locus_tag="KR440_000875"
     CDS             17380..18915
                     /gene="guaA"
                     /locus_tag="KR440_000875"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBH0697938.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTSITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            19454..20884
                     /locus_tag="KR440_000876"
     CDS             19454..20884
                     /locus_tag="KR440_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBH0697939.1"
                     /translation="MILNQIFIEDGGIMRNKIIIGITFSLVLLMSGCASKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGD
                     NNTESYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTY
                     KKNGDSYTKPYIIKNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVGIMEKEKPDIIVAVAHTGEKPKKPRNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGEYISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKGDDMVQIVVMNNNKPVSYIYGEAEVMPINIKCDESEYKNNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(21032..21907)
                     /locus_tag="KR440_000877"
     CDS             complement(21032..21907)
                     /locus_tag="KR440_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0697940.1"
                     /translation="MINIRYLEILEAIEQTGTFTGAAKKLHITQSAVSHAIAELEQQA
                     ETSLFDRLPRGVCLTRCGISLLDEARSILTACRNLDKKISRLEECTPINIVSSITIAS
                     FLLPPILSRLKNSFPSLKVNVRVESANSAMDILKSGEADICFWEGIEPQGAFRTILLG
                     SYKLCVACAPTFPLSEQTLSLRQLCTFPLLLREQGSAIRDTLDSVLSLSNQKAYPVWE
                     SVNSFALIKAAEAGLGITILPENLLLDSLLHKKLRLIELDEIDMENKMLAILHKDKNI
                     TQPLEIILDNISNVL"
     gene            22027..22590
                     /locus_tag="KR440_000878"
     CDS             22027..22590
                     /locus_tag="KR440_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBH0697941.1"
                     /translation="MNQSRKIYFWLLIINLFISTFTFGGGYVVVPMIRRYFVNQKQFF
                     TEDDLMSMAAVAQSTPGAIAINLSALAGYRVAGTLGVVISCTAAIIPPLVILGLVSAF
                     YTTFISNAIVTAVLKGMQAGVAALIVDLIIDMCSMILKQRSLFLSTMIPIAFFANFVM
                     SINVIFILSVCCFVCVMQVFYKKRENK"
     gene            22587..23153
                     /locus_tag="KR440_000879"
     CDS             22587..23153
                     /locus_tag="KR440_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBH0697942.1"
                     /translation="MNTIWNLFFIFLKIGLFSIGGGYAIIPLIQEKVVNQTGWISEKM
                     FTDIITISQMTPGPLAVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKH
                     QNSTYIFEILNGLKSASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFV
                     ILLIVRKWKINPIVIIIISGILGIFFYL"
     gene            complement(23282..23518)
                     /locus_tag="KR440_000880"
     CDS             complement(23282..23518)
                     /locus_tag="KR440_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697943.1"
                     /translation="MLECFIAEDSKIKDGVNIPTLVFKTLHDRYFKTQYFDSYYNSLK
                     CKKKLVEINNVHNSYYIDSEKFCQEAYDWFVENQ"
     gene            complement(23828..27256)
                     /gene="uvrA"
                     /locus_tag="KR440_000881"
     CDS             complement(23828..27256)
                     /gene="uvrA"
                     /locus_tag="KR440_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBH0697944.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFENELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFAKAINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLGNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKTPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNQLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPNAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKISSQNKIIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPNKNYTALTGFDAIDDFILMDQTPIGKSSRSTPATYINIF
                     DEIRSLFTETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCNEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
BASE COUNT        10466 a         3094 c         4807 g         9157 t
ORIGIN      
        1 aagataggag aaaaatgtat gtctaacttt attctaaatg ggatttttag aacagttata
       61 gtaagtagtc taagcattct tctgatattg attttcaaga aaaatgtatt taaaagattt
      121 agcaaaaaat ttaattatta tatatggatg atagttgtta ttaaattatt tttaccattt
      181 acatattata cttttacaat aaatatcttg agaagtaaaa aacacattaa tattaataac
      241 ataaatttag agggatttaa taatgtttca actataagta ataatattat attgtacact
      301 tggattataa ctgtagtagt gtatttaatt tacactatat ttaaatatat aaaacttaaa
      361 aatttaataa atgacttatc ttatgatgtt gatgatagag agattgtaaa tctttataaa
      421 aatatattag aagaatttaa tataacaaaa gatataaaac tcaaatactc ttatgaagtt
      481 gaaacaccag cattttttaa ttcatgtgta cttttgccac cacatgagta taaattgaaa
      541 gagttagact ggatttttag acatgagctt atgcatttta agagtaggga cctttattta
      601 aaatatgtaa tcctgttttt aaaaacagta tattggttta atccatttat atatataatg
      661 gataaacata tagatttaga ttgtgaactt tattgtgatg aaagagtttt aaaaaatcga
      721 aattcagagg aaaaaaagga ttatgcttta acaattctaa atgcaatgag aaaaggttca
      781 aatacttcaa ataaatttat agctggactc cataagcaat ctgatattaa aaagagagtt
      841 atgaatatgt ttaatgaaaa gtacaagaat ggtatattaa tggcattaac tttatgttta
      901 ttatcttcta ttactttttt gaaggttaat tcaacgagta ttgttaatct taatgtatta
      961 cctaaaggat ttgaagactt acgaactgtt agcagtatac gtttttcagt tataggattt
     1021 acatatgcag atgctcctaa aaatatgagg gaagaatata aaataaattg tgaatcatta
     1081 ggtttgatac ctaaagactc tgataaaata gaagttggtg tagaatatta tgacagtcta
     1141 ttgaatgatt aaataaatat tattttaaca gatattaatt ataaagtttt gaatagtaat
     1201 ttgttgagaa ataaatatat ccttataaaa atagatactt atataccaag aataaaaaat
     1261 agttaagata gtacacaagg atatacttat aaagaactta aataagtgaa ataaattatt
     1321 aattataagg tttataattt taaaaaatta gataaattca ggtgtaaaat actataaaac
     1381 acaattcgga aaagtgtatg ttgaacttca tttaaaatag aattttcaaa gttgttatag
     1441 taagtagttt aagcattctt cttatattaa tctttgagaa taatgtactt aaaaaagtta
     1501 gtaaaaaatt taactattgt atattaatga tagttgctat tgatatatta tctaaaaaaa
     1561 ttaagtatga aaagattact gctaggacaa tcttttcata cttaattttt taatatttat
     1621 attctccaaa attttcatct ttttaactta gaattattag aatttgagaa tcactattag
     1681 gaaataggtt ttgatttatg cagggttaaa aatttcacag aatatctaac tttatactaa
     1741 atccatcaaa aaatatacgc catataaaag cgcataaaaa aacacataaa acaatattta
     1801 tgcgcacttt aacctcgaat ggtacaaaat tctttacttt tacagaccca tattatataa
     1861 ttaagtaaat aatgtatatt taggtgacat attggataag ctaatttatt tattttatta
     1921 atataatgat actataaaag ttatataaag tggtttacaa aaaagttaca gatagagctt
     1981 ttattaatgt attgtgtatt agtattttat ccttaggtac acttcaccta caaaatagaa
     2041 tcataagata attatgggaa aattttagga ggtttatcgt gaagaaaaag atattaatac
     2101 cagtatttgc atcagttatg gcattatcag taagttcaat tgtaaatgca gatgaagtaa
     2161 atgattcatc tcaaaataag gatgataaaa caaatactga attaaattta ggtgaatata
     2221 aagaagtaaa atataaagtt gcaaaaataa aagatggagt agctataaag ataagagaag
     2281 aaggtcaagt tcaaaacata gcttattctg gagatgaatt tacggtttta ggaactcaag
     2341 gagaatgggt taaggttaaa gttgaagatg gcgaaggttg gttagctact agatatgtag
     2401 atatatctga aggtgtagga tatactaacg ctgataaagt aaacttaaga aaagataaga
     2461 gtgaaagttc agaagtaatt gaagagctgg aaaaaggaag ttctttattg gttttggagg
     2521 ataatggaga ttggttaaaa gttaaagatg gggaaacaga aggatatgta aaatcatctt
     2581 atatatctga taaagctcct gtaatagaag agccacaagt aaacccaagt attgaccaaa
     2641 atgtagattc tacaaataat aatcaacaaa acaatgcaaa tactaatcaa caaaataata
     2701 attcaaatgt gccaacagct aactctaatg cagttcaagc tgtacttaac ttggcatatt
     2761 ctaagcaagg ttgtccatat gtttggggag cagaaggacc aaacactttt gactgttctg
     2821 gatttacaca atatgtgtat agaaatgcag taggaaagaa tataccaaga acatctaaag
     2881 cacaatctaa atatggacaa actgtaagta aagcaaattt acaaccagga gacttagtat
     2941 tcttcacaac taatggttct ggttctgtta gtcatgttgg aatatatgta ggtgggggta
     3001 atatgataca ttcaccatca acaggaaaaa cagttagtgt aactagtata aattcatcat
     3061 actatacagc aagatttgtt acagcaaaaa gaatattata atcggtttgt aatatgacaa
     3121 attgataaat acataaatat atataaacgc tctatattat atttaattta atcatggaaa
     3181 caaaaaatga ttatatttta aatgtactat agagcgtttt tgtatcactt taaatctaat
     3241 caagattcaa gttaatatga ctattaaata ggagcctttt catatgatga ttatttgcgc
     3301 aatcccttga caggataggg taaaggtggt aagctactaa cagtacaaaa atacttttaa
     3361 ttttagttca gagaggctaa aaaaggagga cgaaagatgt taaaatcaag aaatttaatc
     3421 caaccagaag acttttcaat agaagaaata gatgaaatat tagaattagc tcaaaaaata
     3481 attgacaatc cgtcaaaata ttctcgtata tgtgaaggaa aactattagc tacattattt
     3541 tatgaaccat caacaagaac aagattaagc tttgaatctg ctatgaacag attaggagga
     3601 cgtgtagtag gcttttcaga gccaaattca tcctctgcat caaaaggtga aactctagga
     3661 gatacaatga ggatagtatc tggttatgtt gatataatag ctatgagaca tcctcaatca
     3721 ggtgcagctt cagaagcagc tagatatacg gaagtgccat ttataaatgc aggagacggc
     3781 aaaaaccagc atccaactca aacacttaca gacttactta ctataaaatc attaaaagga
     3841 actttagaaa gccatacaat aggattatgt ggtgatttaa aatatggaag aactgtgcat
     3901 tctttggtta aggctatggc aagatataaa aatacaaagt ttgtatttat agcgcctgaa
     3961 gagttaaaaa tgccagatta tataaaagaa gctataaagg gacatgcata ttatgaaact
     4021 aataatttgg atgatgttat aggaagcttg gatgttttat atatgactag agttcaacaa
     4081 gaaaggtttg aagataaatc agagtatgaa agacttaaaa actattatat tttaaataaa
     4141 gctaaattag aaaaggcatc taaagatatg ttagtaatgc atccattacc aagagtaaat
     4201 gaaatagaca tagatgtaga tagtgatgat agagctgtat attttaaaca agctaaatat
     4261 ggaatgtatg ttagaatggc tttaataata aaacttttag gtataaatga agactaatag
     4321 ttatcatata atagtaaaac aaaggagatt ttgggatgta taaaattctg gaaaatatat
     4381 atataggcga agatatgtat agaatgaaag taaaaggtaa ttttgaaggt aaaatgggac
     4441 aattctatat gttaagagca tgggatactt atccagtact ttcaagacct ataagtatac
     4501 atgatataga tgaagaaggg ataacttttc tttataaagt agtaggagaa ggaactcaaa
     4561 ttttgagtaa cctaaaggta aatgataata taaaattaga aggcccttat ggtaatggat
     4621 atgctaaggt tgatggaaag gtagctctag taggaggagg aatcggtgta gctccactat
     4681 atttggttgc taaaaatata aaaaattgtg atgcatatct tgggtttaga gaagatgtta
     4741 tattagaaga tgaatataag caagtatgta ataaagtcta tacaacagta ggaaatactt
     4801 ttgtaacaga tataattgat gttgagaaat atgattatat attaacatgt ggtccaactc
     4861 caatgatgga aaaattagtt aagatggtag aaggtactaa aacgagaatt atggtatctc
     4921 ttgaaaacca tatggcttgt ggagtaggtg cctgtttagt atgtacgtgt aaaactaatg
     4981 gaggaaacaa aaagacttgt aaagatggtc cagtgttctg gggagaggac gtgattttta
     5041 atgggtaatt taagtgtgaa atttggaagt gtagaattta aaaatccagt aataatggcg
     5101 tctggaactt ttggatttgg aaaagaatat aatgagattt atgatataca aaaacttggt
     5161 ggtataagta gcaagggtct tactttaaat aaaaaacctg gtaacaatgg tatgagagtt
     5221 catgagacac catccggtat gatgaatagt gttgggcttg aaaaccctgg agttcaagga
     5281 tttatagact atgaattacc tttttttagt aaactagact tagtcagaat agctaatgtt
     5341 ggaggtggga cattagaaga ctacttatta ggggttcaaa tgttaaatga caagcctata
     5401 gacattattg aattaaatat atcttgccca aatgtcaaag ctggtggaat ggcttttggt
     5461 ataaaaaatg aagtggcaag agaagttgtt cgtgaggtaa gaaacataac gaaactgcct
     5521 ttagtaataa aattatcacc caatgcagaa gatattgtag gaatggcaaa agtttgtgaa
     5581 gaagaaggtg ctgatggagt ttcattagtt aatactttta aagcaatggc aattgatata
     5641 aaaaatagaa gaccagtatt tgaaaatgta tatgctggat tatctggacc agctataaaa
     5701 cctatagctt tgagaatggt acatgaagtg tgtaaaaatg taaatatacc tgtaatggga
     5761 atgggcggaa taactaaagc cacagatgct atagagttta taatggcagg agctacttgt
     5821 atccaagttg gaactgcaaa ttttatgaat cctagaatag gtattgaaat aatagatggg
     5881 attaatgaat ttatggatag agaaggaata aaaagtttag acgaaataag aggaattata
     5941 taaggtagaa taaatagtat tttaaagata aaaaatatag attaaataaa caataatttt
     6001 ataacattaa catggaggaa aaataatatg agtaaagtag atgtagtgga tatattaaag
     6061 aaaagtgatg cattgttgga gggacatttt ttattatctt caggaaaaca cagtaataga
     6121 tacgtacaat gtgcaaaagt attaagattt ccagaatatg ctgctgaggt attaagtaca
     6181 gttgttgaac aaataaaaga cttagatata gacttagtag taggaccagc tatgggtgga
     6241 gtaatagttt cttatgagtt aggaagacaa ttaggaaaag aagctgtatt tactgagaga
     6301 aaagacaata caatggagtt aagaagagga tttgaagtta aaaaaggagc aaagataata
     6361 attgctgaag acgttgtaac tactggtaaa tcaactatgg agacaaaaag agtattagaa
     6421 gccttaggtg gagaagttgt aggtgttgca tgtatagcag atagaactaa tcatgatata
     6481 ggtatgccta tatatagtgc tataaaactt gatattcaag tttatgaatc tgatgagtgt
     6541 cctttatgta aggaaggaaa attaccagtt gttaagcctg gaagtagaga gttcaaggaa
     6601 ttagggatgt aataaaaact taattattta tagtgttact taaaaaatgt aaatttttta
     6661 gagtaaaaat ttgtaatata acatataact taaaaataaa aagcagttag ccttataaat
     6721 gaaggttagc tgtttttttt gttaaaaata tatttacaaa ataagagata gtagcagtac
     6781 aatataagta taaggtaaaa gtattatata ttagggaggg gtaagtatga aaaatcaatt
     6841 aaatgaaaat cataaaaagg ttgtattttt tggagtaggt gctgtaggag ctacttttgc
     6901 agaacaattt tttaactcta aatatgattt taaaattctt tgtgataatg aaagaaagaa
     6961 aagatattta gaagaaggat ttataataaa tggaaaaagg tatgatttta attatgtaac
     7021 taaagatgag tataaacaag aggctgattt tataattata ggtctaaaat ataataattt
     7081 aaaagaaaat ataaaagaat tagatggatt agttggaaaa aatacagtta taatgtctct
     7141 gctaaatgga gttgatagcg aagagataat aggagaaaga tttggaattg gaaaaatggt
     7201 atattcatat gttaccaata tagatgcaaa gaaaatcaat aataatatta tacacactac
     7261 taatgggata attgtatttg gtaacaaaga taatagtgaa gatagaaaaa ctaatataat
     7321 aactgaagtc tttgatgatg taaatataga atatatttta tcaaaagata ttcaacgaga
     7381 tatgtggtgg aagtacatgg ttaatattgg tgtaaatcaa acttcagcta tacttggtgc
     7441 accttatgga gtttttcaga gttctgagca tttaagagaa ttagcaaaat ctgcaatgag
     7501 ggaagttgtt gctatagcac aagcaaaaga catatctctt acagaagatg atgttgaaaa
     7561 ttcattacat agaatactag aacattcaaa agaaggaaga acatcaatgc ttcaagacgt
     7621 ggaagctcat agacctacag aagtagatat gttttctaag aatatctgca aacttggaaa
     7681 aaaatatagc atacctactc ctataaatca gactttattt tatatgataa aagtaattga
     7741 aagtagattt taaagggtat ttgtaagaaa tattgataat tattgatttt aaattttatt
     7801 aatgttatat actatttaag ttactaaata tttttaactc ttcaatgaaa atagttgctg
     7861 cttttgacaa agttatatct tttggaaata tatatgagaa atgtctgttg tagctgcttc
     7921 caagctctat gacagacagc tcattgttta aaacaggtaa gcttgtcact agattagaaa
     7981 ctattgtaat tcctaaatta tttctaacag attctttgac agcataatta cttccaaaaa
     8041 ccatcatact ttttggaata atttctttaa cacttaaaaa catatctaaa tagtctctgg
     8101 ttccagaacc atcctccctg actatccatt tttggtcttg aagtttatca aaactaaaat
     8161 cttttaataa atgactattg tatggaagtg ctaaaaccat cttatcttca aaaaaatatt
     8221 cttgaataaa tgaaggggaa gagcaagtgc cttcaataag accaatgtct aaaatataat
     8281 ccttaacatg agaagatacg attgatgtat tttttataaa aacttctaca tatatgtcag
     8341 gatacttttg agaaaataat gctaaaaaat ttggtagaat atattctcca atagttaaac
     8401 ttgcaccaat ctttaagtga ccagttatag catctgaaac ttggcttaca tcatgataag
     8461 taatatttaa aagatttaat atttctttag ctcttttgta caatatttgt ccactttctg
     8521 ttataaaaat ggttttttgt ttaatagacc tgtttataag cacaacttta aaataattct
     8581 ctagattttt tatgtgttta cttacacttg gttgtgatag atttaagtat tccccagctt
     8641 tagtaaagtt tttgtattca acaacagcta caaatgtttt taattcttca aacaattttt
     8701 tgtctccttc acctaatgaa attttacatt ttttatatac tttctacaga taattatact
     8761 atatataata accattaaaa acattaatca ttacgatatt atatatttat tttacttatg
     8821 gttattacaa ttatatacta ttgctatgaa gaaaactata ttaattataa ggtaggtata
     8881 ttatgttaaa gaaaaaaagc agtttgacat taaaaactat aagggaaata ttaccaggac
     8941 tgtttatatc agtgttagta ggttatatta gtatatttat ttctatttta attcctaagg
     9001 taggggcagc atcaatttct atatttttag gtatgtttgt aggaaaccta tttttaaatc
     9061 aaaaggtatt tcaaaaaggt tataaatttt cagagacaga tttgttatca tattcaatag
     9121 tactattagg ggcaacactc agtgtatcta ccttaataga cttaaaagtt tcaggaattt
     9181 tatttataat attgcaaatg acaataacaa taatagctgc actttatata ggtaaaaaac
     9241 taggatttga agaaaacttt agatttctaa tggcaagtgg aaatgcagta tgtggttctt
     9301 ctgccatagg agcaatagtt ccagtggtta atgcgacaga taaagaaaag ggaattgcag
     9361 taactattgt aaatgttact ggtatattcc ttatgttttt acttccaata atatctcaat
     9421 ttttatatag ccatgaactt gtaaaaactt cagcaatgat aggtggaact ttgcaatcaa
     9481 ttggtcaggt tgtagcaagt ggagcaattg ttggagaaaa tgtaaaagat ttggctacta
     9541 tttttaagat agtaagagta atatttctag ttgttgttgt attagtattt ggacatatga
     9601 aaaatcagtc taatagggaa atccttgaag aagaaaagga tgagataaaa aagaaaaaag
     9661 taactatacc atggtatgta attggattct ttataacttg tgcacttttt tcaatgaaca
     9721 ttataccaaa agaagtatct gtgttgtgca aagaaataag taacaaacta gaaataatag
     9781 ctttagcagc aattggatta aaagttaatg taaaagattt agtgaaacaa gggaaagaag
     9841 tttctttata tggtttattt gtaggtacag tacaagttgt atcagctgta gttttaataa
     9901 aaatatttat atagcacaaa aatagtattt gtaatattaa tttgattaat taaaataata
     9961 tgatactaat aatttataat ataaataaaa aatgaatttc gataagttta agtgggtata
    10021 aacttataga aattcatttt ttatttaagg taaaaattta aagtaaaata ttattttaca
    10081 taataataac cttataaatt ttgaaattac ttatctttgt gttaaaataa gtaaaataca
    10141 catattaaag ttaagttttt aggcaaataa acaaaatgaa aatataaata ttaaggagat
    10201 ttggtatatg aatgtttatg aaaaaggtaa tggagttata ctagaaggag taacagattt
    10261 tgacccagta catatatttg agtgtggtca atgttttaga tggcacaaac aagaagatgg
    10321 ctcatacact ggagttgcaa gggggagaat attaaatgtc aaaaaagaga atgataaaat
    10381 atatttaaat aatacgaatt taaaggagtt taacagcatt tggtataatt actttgattt
    10441 aggaactgat tatacagaaa taaagaataa gctaaaaaat atggatgaat atttgaataa
    10501 ggctactgaa tttggatggg gaataagaat attaagacaa gatggatggg aaatgcttat
    10561 atcatttata atttcctcta ataatagaat tcctatgata caaagagcga tagaaaattt
    10621 atcaagaaag tttggtaagt atataggtga atatgaaggt aatgaatact atgcattccc
    10681 aacaccagaa gaactaaata aggcctcaca agaggaaata agagcatgtc aaacaggttt
    10741 tagagataag tacataaaaa gtactacaca ggcagttata gagaataatg atgaggtatc
    10801 tgaatataca aatttaagta cagaggattg tagaaaagag cttttaaaat ttaatggagt
    10861 aggaccaaaa gtatgtgatt gtattgcatt atttggtatg caaaaatatg attcttttcc
    10921 agttgatgtt tgggttaaga gagttatgca agaattttat atagatgaag acatgagttt
    10981 acctaaaatg agaacttatg gaatcgataa atttaaagaa atgtctggat ttgctcaaca
    11041 gtacttattt tactatgcaa gagaactcgg tataggaaaa taaattaagg gggagaaatt
    11101 atgggagtaa taggaacaat atttctgttc tatttaatta tatcatatct agctggagcc
    11161 ataatttctg tgattatatt gcttgaaaat agagaccctg caaaaacaat gtcttggcta
    11221 ttgatgttta ttatatttcc aggtgtaggt ttaatgatat atgctatttc aggtagaaat
    11281 attagaaaaa gaaaattatt taagactcaa aaactagcta ataatataaa agaaaagaaa
    11341 ctatttgata cattagaaaa gataacagaa atagttgaat tagaaaaaga atctataaaa
    11401 caaaataagc ttttaagaga tgaagaggat ggtagttata gaaaaagggt aataaatatg
    11461 ttgttaaaga ctggtatgtt tccctttact aaaaataata aagtggatgt atttgtagat
    11521 ggaaatgaaa agtttaaaag acttatagaa gatataagag aagctaaaga ccatattcat
    11581 ttagagtact ttatcatcaa agatagtgaa ataggtaggg tgttaaaaga agaacttata
    11641 aaaaaggcta aagaaggcat taagataaga attttatatg atgatgtagg atgctggaga
    11701 ttttggttta atagaaaatt tttccgagaa atgagggaag taggtataga aattgcagca
    11761 tttttaccta caaaatttcc tataatagga gggaaattaa actatagaaa tcacagaaaa
    11821 attgttgtta ttgatggaat aattggatat acaggtggaa taaatatagg agatgaatat
    11881 ttaggcaaaa atgataagtt tggatattgg agagatactc atattaggat taagggtatt
    11941 tctgtataca tgcttcaaat gacatttctg attgattggt attatacgac aaaagaagta
    12001 ttagtgacta aaaattattt cccaagtgta gggaatgttg gtgaaagtat gatacaagtt
    12061 gttgcgagtg gtccagacag tgactgggaa gatattcatt atgcttattt ttctgcaata
    12121 tgtcaggcta gaaaaaatgt ttatatagaa actccatatt ttattccaga tgaaagtcta
    12181 ctaaaagcta taaagagtgc tgctcttagt ggagttgatg tgagaataat atttcctaaa
    12241 attgcagacc acaagatagt aaatatagca tcttattcat attttgaaga aatattaaga
    12301 gcaggtggta aagtttattt atataataaa ggatttatac attctaaagt cgtaataata
    12361 gatgataaaa ttgcttctgc aggtacagct aatatggatt taagaagttt tatgcttaac
    12421 tttgaggtaa atgcatttat ctatgatgaa gaagttatta gggtaatgac agatgatttc
    12481 tttgaggatt taagtcactg tgaagaactt aatttggagg tatttaagaa tagaaatatc
    12541 atacaaaaaa tcaaagaatc tgtagcaaga ttattttcac caatattata aaaaattatt
    12601 aaaaaaggta ttgaaaatta tgtgtgattg gtatattata ataattgtta gcactctatt
    12661 agatagagtg ataataaata aaaagtatat atataaatct acatatataa gtagtattaa
    12721 tttaaaaata actaaaaaca taggcgaact agtctatact taatatttta ggaggcgtga
    12781 ataatgaaaa taagaccatt agctgacaga gtagtaatta aaaaagtaga agcagaagaa
    12841 aaaactgcaa gtggaatagt tttaccagga gcagctaaag agcaacctca aatagctgaa
    12901 gttgtagaag ttggaccagg tggaatagtt gaagggaaag aaataaaaat ggaattgaca
    12961 gtgggagata aggttatatt ccaaaaatat tctggaacag aagttaagat agaaggacaa
    13021 gaatacacaa tactaagaca gagtgatgta ttagctgtta ttgaataaat atagaataaa
    13081 tttattagga gggatttaaa atggctaaag aaattaaatt ttcagaggaa acaagaagag
    13141 ctttagaagc tggtgtaaat aaattagcag atacagtaaa agtaacatta ggacctaaag
    13201 gaagaaatgt tatattagat aaaaaatttg gttctccact tataactaat gatggagtaa
    13261 ctatagcaaa agagatagaa ctagaagata gatttgaaaa tatgggtgct caattagtta
    13321 aagaagtagc aactaaaact aatgatgtag ctggagatgg tactacaact gctacagttt
    13381 tagcacaagc tataataaga gaaggtttaa agaatgtaac agcaggggct aacccaatac
    13441 ttttaagaaa aggaatacaa aaagcagtaa tagtagcggt agaagaatta aaaaatcaat
    13501 caagaatagt agaaacacaa gaggctatat ctcaagttgc ttctatatct gctggtgatg
    13561 aagaagttgg aaaattaata gcagaagcta tggaaatagt aggtaaagat ggagttataa
    13621 ctgttgaaga gtctcaaact atgaatactg aattagatgc tgttgaaggt atgcagtttg
    13681 atagaggatt tgtttctgca tatatggtta cagatgtaga taaaatggaa gcagttttaa
    13741 atgacccata tatattaatt actgataaga aaatatctaa catacaagaa ttattaccag
    13801 ttcttgaaca aatagttcaa caaggtaaaa agttattgat aatagctgaa gatgtagaag
    13861 gtgaagcatt atctacatta gtagtaaata aattaagagg aacatttgat gtagtagcag
    13921 ttaaagctcc aggctttgga gatagaagaa aagaaatgct tcaagatata gcaatactta
    13981 caggtgctca agtaatatca gaagaattag gttatgattt aaaagaagct gatttatcta
    14041 tgctaggtag agcttcatct gttaaggtaa ctaaagaaag tactacaata gtagatggtt
    14101 ctggagataa aaaagctata gaagatagag taactcaaat taaacatcaa gtagagcaaa
    14161 ctacttcaga ttttgataga gagaaattaa tggaaagatt agctaaactt gctggaggag
    14221 tagctgttgt aaaagttgga gctgctacag aagtagagtt aaaagaaaga aaattaagaa
    14281 tagaagatgc tcttaatgca actagagcag ctgtagaaga aggaatagta gctggtggag
    14341 gaactgcttt tgttagtgtt ataccagcaa taggaacact aattgagagt ttagaaggag
    14401 aagttaaact aggtgctcaa atagttaaaa aagcgttaga agagccatta agacaaatag
    14461 ctataaatgc tggtcttgaa ggtgctgtaa ttgtgcaaaa tgttgttaat tctgaagcag
    14521 aaactggatt tgacgcattg aatgaaaaat atgtaaatat gatagaagct ggtatagttg
    14581 acccaactaa agttagtaga agtgctttac aaaatgctgc atcaatagca agtactttct
    14641 taactactga agctgctgtt gctgaccttc ctgaaaaaga agatgcagga atgccaggaa
    14701 tgggtggcgg aatgcctggt atgatgtaat ttaaaaaatt taaaatataa attttaatat
    14761 aaagaactat ttcgacttag attttaaact ctaagttgaa atagttcttt tgtatatatc
    14821 taatttttgt atatctataa attcaataca catatttaga taatcagtat ctagtttgta
    14881 ataatatttt agagtagtat tgttattttt taggttatga ttttagatat atattaatta
    14941 aggtataatt gttgatagag aataaatcaa atttaaaagg atgcgataat atggacttaa
    15001 gaaaattact tgaagaagtt aaaaatgaaa atattgatat agattttgcg ttagaaaagt
    15061 taaaagattt accttatgaa gaccttggat atgcaaatat agaccatcat agagaattaa
    15121 gaaatgggta tccggaagtt atttattgtg aaggtaagag tgatgagcat attttaggga
    15181 ttattgataa aatgaatcaa aaaagttcta acatattggg gacaaggtgc agaaaagaaa
    15241 cttttctgaa aattaaagaa atctataatc atgctgagta cgaagaggcg tctaaaattc
    15301 ttaagattca aaatcatgat atagagaata taggaaaagg taaaattgtg attgctacag
    15361 ggggaacttc tgatatacca gtagcagacg aagcttatca tacagctaag tttttaggta
    15421 atgatgttga tagaatatat gatgttggag tggcaggtat acataggctt ttaaataaaa
    15481 gacataaaat agatagtgct agagtaatag tagccgttgc aggtatggaa ggtgctcttg
    15541 caagtgtaat tggtggactg gttgatgttc cagtaatagc tgttccaact tctgttggat
    15601 atggagcaaa ttttggagga ttagcagcat tactagctat gttaaatagc tgtgcatctg
    15661 gaatatctgt tgtaaatatt gataatggat ttggagcagg atatttagca tcaatgataa
    15721 ataaactgta aatacattga tataaattaa acaaaactat ataataaaat ttaatttcta
    15781 ttatattttt actattaaaa ttgaggagac atatatgagt gaaaaaatac tttattttga
    15841 tataataagc ggtatatcag gggatatgac ccttgcaagt ttattaaatt taggtgtgcc
    15901 taaggaaatt tttttggagg aaattcataa actaagaatg agcgatgaat ttaatataaa
    15961 catcagctcg aaaactgaaa atggaatagt aggtacaaaa gtagaagtaa ttacaaaaga
    16021 aaaacataca catagaaatt tagtggatat atttgaggtt atagataaaa gcactctaaa
    16081 cgaaaatgta aaatctaaag caaaaaaaat atttatggta attggagaag cagaagcaaa
    16141 agttcatgga actacaatag acaagataca ttttcatgag gtaggggcta tagattcaat
    16201 tgtagatata gtaggagctt ctatattagt tgacttactt tgtgtagata aagtttgtgc
    16261 tagtacagtg tctgttggtt caggatttgt aaaatgtgaa catggaatta tacctatacc
    16321 tgcacctgca actatagaaa ttttaaaagg tgtgccaatt aaattaaata atgtaaatgg
    16381 agaatgtaca actccaacag gagctgcaat cataaaagtc ttgtgtgatg agtttgtaga
    16441 tgagcttgaa ttttctccaa agcaaattgg atatggtata ggtcataaga agtttgaagt
    16501 accaaatata ctcagaactg tattaggtga aaaaaaaaag aagaattaat ttatgaaatt
    16561 agtgcaaaca ttgatgatat gtcttcagaa atctattctt atttgtatga aaaaatatta
    16621 gatgagaaag cattggatat ttatacagag agtatttata tgaagaagaa tagaccagca
    16681 acaaaagtat caattttatg taatgaatca gatttagaaa agtttattaa aatattactt
    16741 ttagaaacta gtacttttgg agtgagatat aatgcatata atagagaaat tttagagaga
    16801 aaatttgtaa aattagatac aaaatatgga gtggttacag taaaactagg gtattataaa
    16861 ggaaaactag tcaaggttac tccagaatat aatgaatgta aaactatagc aaaaaatgaa
    16921 gatattccta taaaaaaagt gtttgctgat ataaatttta gaataaaaga agaatttagt
    16981 gaaaacttat tgacataagt aataaatttt gataaattaa tattcagcct tattaaataa
    17041 aagtaaacag aaaaaaatat ataaaatata aactcactcg tatatgctca gaaatatggt
    17101 ctgagagtct ctaccaagat accgtaaata tcttgactat gagtgaaatt attataccaa
    17161 aagacctagt taagtaaatg tgtaggctga gttatactcc ctatgtgttg gctataagat
    17221 aaaggtattt tggaagcctg tatagtaatt tcacttttgc tagaaattat ggaagttact
    17281 atacaggttt tttgtgtaaa aagatttaat ataatttaga ctgtattgtg ttaaaataaa
    17341 tatataaaaa ctatattata caaggagaga tgtgaattta tgaaacatga attagtactt
    17401 gtaatagatt ttggaggaca atataatcag ttgatagcta gaagagttag agaaaacaac
    17461 gtatattgtg aaatacttcc atgtactgca agcatagaaa gaataaaaga aaaaaatcct
    17521 aaagggataa tatttacagg cggtccaaat agtgcgtatt tagaagattc tccaacaata
    17581 tcaaaagaaa tatttgaact aggagtacca atacttggaa tatgctatgg tattcaaata
    17641 atgtctcatg tattgggtgg agttgtaaga aaaggtaata aacaagaaaa ggaatatgga
    17701 aaaacatcta taacttatgg aaaatcatca ttatttgaag gaataactac aaatagtgta
    17761 tggatgagtc atacagattt aatagaaaaa gttccagaag gatttactat agttgcaaat
    17821 actaatgatt gtccagtagc agctatggaa aatgtagaac gaaacctata tggagttcaa
    17881 ttccatccag aggtagaaca ctgtttggaa ggggacaaga tacttacaaa cttcttatat
    17941 aatatttgta aagtaaaagg tgactggact acagattcat tcatagaaga taaaataaaa
    18001 gaattaaaag aaaaaatagg cgataaaaaa gctttatgtg ctttaagtgg aggagttgat
    18061 tcttctgttg ctgcagttct tattcataaa gcaattgggg acaacttaac ttgtatattt
    18121 gttgaccatg gtttacttag aaaaaatgaa ggaaatgatg tagaaagaat atttagagag
    18181 aaatttgata taaatttaat aagagttaat gcagaagata gattcttatc taaattaaaa
    18241 ggagtttccg aaccagaagc taagagaaaa ataataggtg aagaatttat aagagtattt
    18301 gaagaagaat caaataaatt aggaaaaatg gatttcttag tacaaggaac aatctatcca
    18361 gatgttatag aaagtggaca tggaaatgct gctactataa agtctcatca taatgttggt
    18421 ggaatcccag aagatgtaga cttccaagaa attgtagaac cattaagaga gttatttaag
    18481 gatgaagtta gaaaaatagg attagagctt ggtatagaag aaggattaat atttagacat
    18541 cctttcccag gaccaggtct aggaataaga gttataggtg atgtaactaa agaaaaatgt
    18601 gatatattaa gagaagctga tgctgtttat atggacgagc ttagaaaagc aggtttatac
    18661 agagaaatat ggcaagcttt tgcaacatta ccagatgtta agactgttgg agtcatgggt
    18721 gatgaaagaa catatgctta tttagttggt ttaagagcag ttacttcttc tgatggaatg
    18781 acttctgact ggtataagat gccttatgat gtattagaaa gaatttctaa tagaataata
    18841 aatgaagttg atggagtaaa tagagtagtt tatgatataa cttctaaacc tcctggaact
    18901 attgaatggg aataaaatat agaatgccag aaatccacat gaatacagga tttgaggtct
    18961 ctcttacttc tatattatac tggtttgata ctatcaatat ttgccagtat cttatggatt
    19021 aataagcaaa aaacatcctg ctgaatggta aattcggcgg ggtgttttta ttttgttttg
    19081 aaactccttt gctctgaaac tcgaagtatt taacactgat gatgttgtta tcttttagtt
    19141 ccttttgtgt catgctctga aagacaaatt gatacaataa aaaagtaatt tcttaatcat
    19201 tgatggagat atgtatttat gttactcttg atggtaattt gagtaatatt gttcatagct
    19261 ttttatattc acttaattag aaaaccattt ggtataatca taaataaact tgatgatata
    19321 atagaaaaga tttataatta ttagaagtat ataaaagtat tttatgtcta tgaaatgtat
    19381 gtctataaaa catatagatg acttttgtat attaatgtat gaatattgat attatagaag
    19441 aattgagttg tcaatgatac taaatcaaat atttatagaa gatggaggga ttatgagaaa
    19501 taaaataatt ataggaatta ctttttcact tgtattacta atgtctggat gtgcttcaaa
    19561 acaaacagca gaaataaacg tattagcaac tactgattta catggagtta taccttatga
    19621 gttaacttct tatgtaaaag aagaaagaaa aaaagataaa aatctaactt tagtagatgc
    19681 aggagatttt tttgatagtg gagaagtgtt tggttctagc atggatagtt attttagtgc
    19741 tagatataaa gatggtgata ataacactga aagttatatt gagacgccta tttcaaaaga
    19801 tatgaaagag gtaggttatg atgttgcagt tcttgggaat catgaattta tatccaacaa
    19861 taaattttat ctagataata tgatatctga ttttgagaaa caaaaaataa atattttatc
    19921 ggcaaatacg tataaaaaaa atggagatag ttatacaaaa ccatacataa taaaaaatat
    19981 taatacacct gaaggaaacg taaaactagg aattttaggg cttactataa aagaagttgg
    20041 tgagaggaaa cagtggaaag atggaaaact tgttgactct aagtctttag aactaaaaga
    20101 ccaagagggc tacaatggtg aattgtatat gaatgattta gtagaagatg ctaaaaagtg
    20161 ggttggtata atggaaaaag agaagcctga cattatagtt gctgtggcac atacaggtga
    20221 gaaacctaag aaacctagaa acccaggaaa tagaatacaa gacttagctc aacaagttga
    20281 tggaattgat gctatagttg caggtcataa tcatgttcaa attgagcaac atgattataa
    20341 aaataaatca ggagaaaatg ttatagttac tgagccagga aagcatgggg aatatatttc
    20401 taagattaat tttaaattag aaaaaaacaa tgataaatgg aatatagttg acaaatctag
    20461 taaattaatt aaatttgaaa aaaatcaaga aagtgataaa tttggagaat ttatttcttc
    20521 aattaataaa gtaagtaaag ataagaaaga aataagatta tcagaagtaa agactcctga
    20581 atgggataaa atatatgttt ttaaatctgg aacatctgca gatgatatat ataaaacaat
    20641 aggatataaa tggagaaata tatcatctcc aaagggtgat gacatggtgc agattgttgt
    20701 aatgaataat aataagcctg ttagctatat atatggtgaa gctgaagtta tgccaattaa
    20761 tataaaatgt gatgaatcag agtataaaaa taatgttgta actatttatc ctaataaaaa
    20821 tgataaattt aaaattgaaa aaggtaagca agaatatagt attaatttaa aacatataca
    20881 atagaaactt ttatcttatt taagtttaga ttgtacttaa aataaagtgt gtattatttt
    20941 aaaaagtgat gataattaaa atcacaacat taaggaatag tacacacttt ttatgctaaa
    21001 aaatagaatg ttaaaaaatt aaacctttaa attaaagtac attgcttata ttatccaata
    21061 ttatttccaa aggttgtgta atatttttat ccttatgtaa tattgctaac attttatttt
    21121 ccatatctat ctcatctaat tcaatcaatc gcaatttttt atgtaagagt gaatctaata
    21181 ataaattttc tggcaaaatg gttataccta accctgcttc tgcagcttta ataagtgcaa
    21241 aagaattaac actttcccaa acaggataag ctttttggtt tgaaagagat aatacactgt
    21301 ccaaagtatc gcgaatagca cttccttgtt cacgtaacaa taacggaaac gtgcataact
    21361 gtcgtagaga taaggtttgt tcagataaag gaaaagttgg agcacatgca acacataact
    21421 tataggaacc taataaaatt gtacggaatg ccccctgtgg ttctatacct tcccaaaaac
    21481 atatatcagc ttctccactt tttaaaatat ccatagctga attagcactt tcgacacgta
    21541 cattcacttt aagagatgga aatgaatttt ttagtcgact taaaattggt ggaagtaaaa
    21601 aagaggcaat tgtaatgcta gaaacaatgt ttataggtgt acattcttct aaacgactga
    21661 tttttttgtc taaattgcga caagcagtta agatactacg tgcttcatct aaaagagaaa
    21721 tgccacagcg agtaagacat acccctcgtg gtaatctgtc aaatagtgat gtttcagctt
    21781 gttgttcaag ttcagctata gcatgagata cagcagattg tgtgatatgt aatttttttg
    21841 cagcacctgt aaaagtgcct gtttgttcaa ttgcttctaa aatctctaaa tatcgtatgt
    21901 ttatcataat aaatatcctc actatacatg aataaaatta attaattcca ttaaattcta
    21961 tcattttaca gattgcaaag caacaggtat actgaataat atgaagtgaa aagaaggagg
    22021 aatattttga atcaaagcag aaaaatatat ttttggcttt taattattaa cttgtttatt
    22081 agcacattca cttttggcgg agggtatgta gttgtaccta tgattagacg ttattttgta
    22141 aatcaaaagc aattctttac agaagatgat ttgatgagta tggcagcagt tgcacaatca
    22201 actcctggag ctattgcaat taatttatct gctttggcag gttatcgagt agctggtact
    22261 ttaggggttg tcattagttg tacagcagct ataatacctc cactcgttat tcttggatta
    22321 gtatcagcat tttacaccac ttttatatct aatgccattg taactgcagt attaaaagga
    22381 atgcaagctg gtgttgctgc actaattgtg gatttgatta ttgacatgtg ctctatgata
    22441 ttaaaacaac gttctttgtt tttatcaact atgataccta ttgctttctt tgcaaacttt
    22501 gtgatgagta taaatgtaat ttttatatta tctgtttgct gttttgtatg tgtaatgcaa
    22561 gttttctata agaagaggga aaataaatga atacaatttg gaacttattt tttatatttt
    22621 taaaaatagg cttgtttagt attggtggtg gttatgcaat cataccttta atccaagaga
    22681 aagtagtaaa tcaaactgga tggataagtg agaaaatgtt tacagatatt ataactattt
    22741 cacaaatgac accagggcca ctggctgtta atacttctac ttttgttgga cttcaaattg
    22801 gagggttttg gggagcagtt gcagccactt taggttgtgt attatgtggt gtagtaatat
    22861 ctctattttt atataatttt tttcaaaagc atcagaactc tacttatata tttgaaatat
    22921 taaatggtct aaaatcagct tcattaggtt taattatctc agctgcggct acaattataa
    22981 tccttgcatt atttgggacg aataaagtgt acttcaattc tatatttgag atgctaaact
    23041 ggactgcact aataatcttt tttgttatac tactgatagt acgtaagtgg aaaattaatc
    23101 ctattgtgat tataataata agtggtatac taggaatatt tttttattta taagtttttg
    23161 gtaagtaaag gatatctatt tttttatatc ttaaatccaa atagccaata aagtaaggct
    23221 atttggattt ttaataaatt ctgaaaagat aatcagaaac ccatataaca tttaaattat
    23281 atcactggtt ttctacaaac caatcataag cttcttggca aaatttctca ctatctatat
    23341 agtaactatt atgaacatta ttaatttcaa caagtttctt tttacatttt agtgaattat
    23401 aatagctatc aaaatattgg gttttaaaat atctatcatg aagtgtttta aagacaagag
    23461 taggtatgtt gactccatct tttatcttac tatcttctgc aataaagcat tctaacattg
    23521 tattaattat ctttgtacta tttttccaaa tatgaggatt tttcttttca aatattttat
    23581 aaaattcatt atcatagact tcattataac ctttatattt aggtggtttt actgttatgt
    23641 gaaaattagg aagaaactta aagaaaaaat ataataattt ttatgttctg ttttatatat
    23701 tattcatatt ttatcacaga taatatacta aagttaattt ttttattgta caactattat
    23761 aataattctt ctataatata agacattcta taattttata tttaagttca tgtgtattta
    23821 attgttttta tataaaatgt ccagtaatac tatttggatt tatttttatt tcatcaggag
    23881 ttccagaagc aacaatttcc cctccattta ttccactgtc tggacctata tcgataatcc
    23941 aatcagaagc acgtataacc tctgtattgt gttcaataat taaaagtgaa tgctgttcat
    24001 ttacaagttt tctaaaagta atcaacaaat tttctatatc tttaaaatga agtccagtag
    24061 taggctcatc caatatgtac agcattcgtt ttttagtttt tgtccctaat tcttttgcta
    24121 attttatcct ttgagactct cctccgctta atcctaatgt attttgtcca agctttagat
    24181 atccaagtcc cacttcatct aataaggaga gttttctata tatatcagag caatcactaa
    24241 aaaatgtttt tgcttctgct acttccatgt caagtatatc tgcaattgtt tttcccttgt
    24301 attgtataga cagtatttct ttttgatatc tcttaccttg acattcatta caagttaccc
    24361 attggtctgc catatattgt agaataattt ttgttttacc taatccttga cagtttgggc
    24421 attgcccctt tttgctatta aagctaaaat agctttcatc caataatttt tgttttgctt
    24481 gtggagtttc agtaaataga gaacgaattt catcaaaaat attgatatat gtagcaggtg
    24541 tagaacggct agattttcca ataggtgttt ggtccattaa gataaaatcg tcaatagcat
    24601 caaatccagt aagtgcagta taatttttat ttgggataga tttttgtttc aatttttctt
    24661 ctagagcagg aagtaaactg tgaaatacaa gagaactctt accagaacca ctaacacctg
    24721 taatagagca catacagttt aagggaattt ctacatcaat attttttaag ttatttgcat
    24781 ggcatccctt taagcttaac catttacttg gtataatttt attttgtgaa cttatcttat
    24841 tgtatgtact caaatatttg cctgttatag aatttggatt ttttataatt tcttgggttg
    24901 taccttctgc aatgatgaag cccccttttg taccagcatt tggtcctaca tcaataatat
    24961 aatcagcaga cagtattgta tccttatcat gttccactac tataacagta ttacccttgt
    25021 ctcttaattc ttctatcatt ttgatgatta gattatggtc tcttggatgt aaaccaatag
    25081 atggttcgtc taaaatatag gttaatccta ctaattcact tcctagctga cttgataatc
    25141 gtactctctg cagttctcct ccagataaag ttggagctgt tctatctgtt gatatatagc
    25201 taagacctac ctttaataga taactaactc gaatttttaa ttctgttaag atatcattta
    25261 ccaaagatag ctcatttttt tgaagttgat ttggtaattc acacaaccaa ttccataact
    25321 gttctatcgt catttttgtt agttcaggga agcgataacc tttgattgta gtgaatctag
    25381 cctcaatgca cagcaattcg ccaccacatg tattgcaagg aattttgttc atatattgat
    25441 gcaaggtatt attttcagaa ctagattctc taaaaagtct ttgaatgtgg ttgattgctc
    25501 ctgaagcagg ccttctaatc tttgattctc gtccctttga ttcaaagcta aattcatata
    25561 tcttatcgtc tgtaccatat aagattgcat ctataaaatc tcttggcaat tcattccaag
    25621 gtatatctag gtctatattt ttatcttttg ctattgcata taattcacct accatccaat
    25681 taccatttgg ctttttcccc ctcaattttc caaaatagta agtagctcca tctaatattg
    25741 aaatagttgg gtcttttaca attaattgag gattaacttg ataggtaaaa cccaatccat
    25801 tacaatcgtg acaagctcca gtatgggagt tagcattgaa ggttgaagta tttatcttag
    25861 aaaaaatagt ttggcaatgt ggacaataat tttttgtatt attctttttt tctaatggag
    25921 ttttgcaata tggacagtaa ctttttccga ttgatacaaa taacattctt aagtaatccc
    25981 aaatgcttgt aagagtacca acagtagaac gaggattttg gcttcctttt ttctgttcga
    26041 tagctatagt aggggttaat ccctgaagta aatcaaattc cggtttttga agctgttctg
    26101 aaagttttgc tccattacct agattttcaa aataacgtct ttttccttct tcataaatcg
    26161 tatcgaaagc taaagaagat tttccactac cacttacacc tgtaataaca gttatttgat
    26221 tttttggaat tgtaacctca acatccttta aattgttttg ccttcctcct tttagataga
    26281 tagagctttt attatgacaa agtgaatttt taattgattt atttattgct tttgcaaatt
    26341 tgaatttctt ttctagttca agaggattgc ctaattcttt tatttccgaa atacctaata
    26401 tttttaactg agaaaagatt gatttcaaat aatcttcagg caatttttct attaacaaat
    26461 taaggtcttc tatttttata tttccgcttg ataatatctc tgccatttca tctaataaat
    26521 ttttttctgc tcttgttaat tgattgtatt ttttggaact taattctgtc cataaatccc
    26581 atggaagcaa ttctaaacca atcatattat taaagtcatg tagaagatta ccctttacag
    26641 aatgccaacc tttccaattt ttattatatc caaagtcatc tggatgagca tgtccttgtc
    26701 gacatcttat ccaggcttct gaaccagtta aaaaaccatc tgtttttccg acatcatgag
    26761 tgtcaaaatt aatttgaaaa taattttttt gtatgtcatc tatttgtgca tctattctta
    26821 tccatctctt tgttagagta tcatagtatt ctacaagcca gtggtcttca taagtaagct
    26881 cattttcaaa ataattagca aaacctgctc tcattctagc ctcaaaacct cgatatctta
    26941 ggagcgatac aagaagtaaa gaataatctc tgcacattcc aacaagcctt tgttttgggt
    27001 ttcttggaat agttagtggg ttagaattta tttcaaatat tctttttaac atttgaggaa
    27061 ttgtacgaag caattcctca tcagattgtt gcttattaaa ggatatacca tataattttc
    27121 cttggtctcc atgaattact aggctttgaa ccacttttaa taactcaatt ggagtctttg
    27181 gtaaatcttg atatttattt tcaaattttt taggatttgt gatgatgctt tgatttttat
    27241 agttatttat tttcatttat atttcacctc tctaaatgga tgatataccc tgacatggtg
    27301 tcatatgcaa ggacaattta gattttttgg ccaaaattta aaaataaata aaaaatgata
    27361 cagtgataaa ttgtaataca aatatagttt caaattgcat ataatataat tatttaataa
    27421 aaccattatc tatggaatgt taatgaaatg ttaagatggc attcttgaat attaagaaat
    27481 agaatactat aatatagtgt gaaggattct tagtaaaaaa attt
//
LOCUS       DAEPOZ010000025        27438 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.025, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000025 DAEPOZ010000000
VERSION     DAEPOZ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27438
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.025"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(50..721)
                     /locus_tag="KR440_000882"
     CDS             complement(50..721)
                     /locus_tag="KR440_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBH0697945.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
     gene            complement(753..1364)
                     /locus_tag="KR440_000883"
     CDS             complement(753..1364)
                     /locus_tag="KR440_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBH0697946.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            complement(1402..2205)
                     /locus_tag="KR440_000884"
     CDS             complement(1402..2205)
                     /locus_tag="KR440_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH0697947.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINKTLKEMKKDGTFDKIYDKWFK"
     misc_binding    complement(2331..2593)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(2679..3986)
                     /locus_tag="KR440_000885"
     CDS             complement(2679..3986)
                     /locus_tag="KR440_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697948.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLVLDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYHNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQSKILTDEAFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     gene            complement(4617..5057)
                     /locus_tag="KR440_000886"
     CDS             complement(4617..5057)
                     /locus_tag="KR440_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HBH0697949.1"
                     /translation="MKNLNLLINKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFL
                     EMIDNENSYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPN
                     ITKYKENLINRIKMELSNADISIYNNNMKPLVEKDINDTLSNIS"
     gene            complement(5263..5640)
                     /locus_tag="KR440_000887"
     CDS             complement(5263..5640)
                     /locus_tag="KR440_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBH0697950.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
     gene            complement(5756..6250)
                     /locus_tag="KR440_000888"
     CDS             complement(5756..6250)
                     /locus_tag="KR440_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBH0697951.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSVYIGDEKFNNLKNECIKK
                     GIDIKISKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKNIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            complement(6399..10652)
                     /locus_tag="KR440_000889"
     CDS             complement(6399..10652)
                     /locus_tag="KR440_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBH0697952.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKISYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDVPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            complement(11033..12202)
                     /locus_tag="KR440_000890"
     CDS             complement(11033..12202)
                     /locus_tag="KR440_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697953.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYNPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            complement(12597..13613)
                     /gene="gap"
                     /locus_tag="KR440_000891"
     CDS             complement(12597..13613)
                     /gene="gap"
                     /locus_tag="KR440_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBH0697954.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYSDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNQKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            complement(14250..14714)
                     /locus_tag="KR440_000892"
     CDS             complement(14250..14714)
                     /locus_tag="KR440_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 family lipoprotein"
                     /protein_id="HBH0697955.1"
                     /translation="MKKLVIILISIISVVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTDETLWSNTWH
                     GSVDNDTFAISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
     gene            complement(15038..15646)
                     /locus_tag="KR440_000893"
     CDS             complement(15038..15646)
                     /locus_tag="KR440_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBH0697956.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            complement(15728..16429)
                     /locus_tag="KR440_000894"
     CDS             complement(15728..16429)
                     /locus_tag="KR440_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0697957.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            16656..16922
                     /locus_tag="KR440_000895"
     CDS             16656..16922
                     /locus_tag="KR440_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBH0697958.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSMIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            complement(17025..17444)
                     /locus_tag="KR440_000896"
     CDS             complement(17025..17444)
                     /locus_tag="KR440_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HBH0697959.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERIFNLKQEKRKAKHKGH"
     gene            complement(17711..18493)
                     /locus_tag="KR440_000897"
                     /pseudo
     CDS             complement(17711..18493)
                     /locus_tag="KR440_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
     gene            complement(18558..19319)
                     /locus_tag="KR440_000898"
     CDS             complement(18558..19319)
                     /locus_tag="KR440_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBH0697960.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLARILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNKKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            complement(20368..21180)
                     /locus_tag="KR440_000899"
     CDS             complement(20368..21180)
                     /locus_tag="KR440_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH0697961.1"
                     /translation="MYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPVNYPNLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSINYAQNRN
                     KETNLNINYLFQSYLNMNYNDEFDLATLIYCDYGALSTENRRLLMEKIYDSLKPGGKL
                     ILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTLVITEKD
                     ENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK"
     gene            complement(21550..22452)
                     /locus_tag="KR440_000900"
     CDS             complement(21550..22452)
                     /locus_tag="KR440_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH0697962.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSAGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDGQISFFD"
     gene            complement(22502..22963)
                     /locus_tag="KR440_000901"
     CDS             complement(22502..22963)
                     /locus_tag="KR440_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HBH0697963.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            complement(23015..23470)
                     /gene="def"
                     /locus_tag="KR440_000902"
     CDS             complement(23015..23470)
                     /gene="def"
                     /locus_tag="KR440_000902"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBH0697964.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            complement(23442..23705)
                     /locus_tag="KR440_000903"
     CDS             complement(23442..23705)
                     /locus_tag="KR440_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0697965.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            complement(23802..24518)
                     /locus_tag="KR440_000904"
     CDS             complement(23802..24518)
                     /locus_tag="KR440_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0697966.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGTLAFIITMILLIFFSLTKISFLLNLSISLLVLLQGVIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYIYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            complement(24515..25192)
                     /locus_tag="KR440_000905"
     CDS             complement(24515..25192)
                     /locus_tag="KR440_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0697967.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLILASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILISIIIIIYFITYYFICSSWK
                     KVGGVKL"
     gene            complement(25189..26034)
                     /locus_tag="KR440_000906"
     CDS             complement(25189..26034)
                     /locus_tag="KR440_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0697968.1"
                     /translation="MIKVDDLSFSYTDRNFLQNINFEVEKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENEANKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIKKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            complement(26148..26738)
                     /locus_tag="KR440_000907"
     CDS             complement(26148..26738)
                     /locus_tag="KR440_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0697969.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEHFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRVVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            27027..27260
                     /locus_tag="KR440_000908"
     CDS             27027..27260
                     /locus_tag="KR440_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBH0697970.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
BASE COUNT         9054 a         4682 c         2618 g        11084 t
ORIGIN      
        1 ccctttattt atattataaa attctaccct agtttatatt tataaaactc taaacaattt
       61 tttcttttat ttgaacattt tctactatac catcttttat acttataatt ttatctgcaa
      121 cattatttga gaagacaaca tcatgtgtaa ttattaatat tgtcatacct ttgtctttaa
      181 gtctattcat tatattgact actctttgaa tagtatcaat atcaagtgca gaagtaggtt
      241 catcaaaaca aaggactttt ggcatcaatg cacatgacct agctatagca actctctgtt
      301 gctgtcctcc tgacaattca aatggataag aatttaattt atcttctaaa tcaactaatc
      361 ttaaaagttc tctagccctc tcttcagcct cttttctagg aactttaaaa acattaactg
      421 gggattcaat tatattttca aggactgtca tatgaggaaa taagttgaaa ttttgaaaaa
      481 ccatacctat ttttcctcgt atttttgcca tatctctctt attttttatt acctcattat
      541 ctattataat ctctccacta tcaaattctt caagaccatt tatacatctt aatattgtgg
      601 tctttcctgc accagactta ccaagtaaaa ctccaatctg accttcttct aactcaaaag
      661 aaatatcttt aagtactcta ttttttttaa atgatttatt taagtttttg attttcaaca
      721 tataaaaaac ctcctcctat aatattgtaa ctttatctat aataagaata ttttttttca
      781 agtaattcaa atcccttagt aagtatagct acaaatataa gatatatcgc tcctgcctca
      841 aataatggta acagacttgc ttccctgttc attgctattt gagatattct taaaatatca
      901 tttaatccta atatatatac taaagatgta tcttttacta atgtgattac ttcatttgaa
      961 ataggtgcta gtattctttt aaaaacttgt ggaaagatta ctctcttata catagtaaat
     1021 ttatcaaatc ctagtacttt tgaagcttca tattgtcctc tatcgattga ctgtattcca
     1081 cctctaaata tttctgcaaa atatgctgca taatttaaga agaaagctac tactccagct
     1141 gtaaatctgt caaatacaat tcctaaaaga ggtaatccgt aaaatatgac tatcatttgc
     1201 aaaagtaaag gtgtacctct cattatcaat atataacatt gtgtaatacc acttactaat
     1261 ttattctttg ataatcttaa aaatgcaact ccaatcccca atggaattga tactatcaat
     1321 gttatacaaa acattgctat aactatctct aaaccttgta acatgccttt agtgccccct
     1381 tcttaacagc taacttatct tctatttaaa ccacttatca taaattttgt caaaagttcc
     1441 atcttttttc atttctttta atgttttatt tattttctca caaagttctg gattttcttt
     1501 agaagttgct acaacataat cttctaatcc aaaatcgtct tttaatacct tgtatttgtc
     1561 ttcacccttt tgtcccatat aatatctaat taaaacttca tcaccaacaa ctgcactagt
     1621 tctacctatc tctaaatctc taagtgcttt atcataagta tcataaagaa ctggtgctcc
     1681 accttttaag ctattcataa aatctttatc cttttccact gcatcaagtg ctgttgaacc
     1741 ttgttgtgtt ccaacttctt tatcttttaa atcagctttt gaatttattt ttgaatcact
     1801 tagagtaact attatctgtt tattttgtaa atatggttct gtatatgata ctattttctt
     1861 tctttcgtct gttatagaat atccattcca taatacatct acagtttttg aatcatttaa
     1921 ttcagtttcc ttcattgacc aatctatagg ctgaaactta acttccattc caagtctttt
     1981 aaatgtttct ttagctaagt ctacgtcaaa tcctactgta tctccctttt catctaaaaa
     2041 tcccattggt acaaacgtat tatcaaaccc tacaactact tcttttttag attcctttga
     2101 agcatcctta tctttttcat tatctggttt tgaacaacct acaactccac ctaataaacc
     2161 taacataatt gtaaatattc ctactttttt taatatattt ttcataatat tctccctcgc
     2221 cttttaattt ttaaaatatt ttgattattt tttaaaaagt gtatctattg caatataatt
     2281 ctctgcgcgc ttaaaaaatt atattaaaaa actcccgcct ctagcttctg ctagaggacg
     2341 ggagttaact ctcgtggttc cacctctgtt cattaatgcc tcacgacact aacctcttta
     2401 agtacaataa aattattata ctctagcact ataacgggtg caaggattcc ggaaatctcc
     2461 taataaagta tttctactct ttcagagtcc tgctcaaaga tgtgttcaaa gtaaaatcta
     2521 ttgctcctct caccaatcgg aaactctctg tttaagatta actaccttta ctatttcttt
     2581 tcttagcatt taatgttatt ctgaattatt attatgatat tgattttata gcataaggca
     2641 atatatgtca atatatattt taaaataaat ataaaaatct agttccaagg tacaacttcc
     2701 acttcaccat aacacctttc tactaatctg tttgaatatt ctcctctata acctaaaata
     2761 aagagtaaaa caagttcaat atatctcgtt cctattgaaa ttatattcca aatatcttca
     2821 acctctaaca cagctctatg agtctttcta cttggatgaa ctatgctatt tcttaaataa
     2881 ataactaaat ccactccatc atcaaattta ttttttatta tattatcaaa tatatttaat
     2941 tcatgtttac catatggtat cttacatgta tcaagtaaga gtcttatatt tttagatgcc
     3001 aaattacagt caaatgcttc atctgtaagt atcttacttt gttccactag aattacatat
     3061 gacaaagttt caagtgcaat ttgtacagat attatattgt tttccatggt agcacttcca
     3121 agagattcta tgtaccaatc aacaacactc tttatagcag aaccataata aaaatcttct
     3181 agctttttac acataaggct tatatatttt tccatattat gataatttga aagtgtatct
     3241 gaccatgttg gtacatatct aaaaggagat atttgatttt cattccatat tctataaact
     3301 tcattaccac ttctataccc tttggccaaa caaaacccta catatcttcc acacataaaa
     3361 ctcaaagctg ttgatattct atctaaaaga tttattgtat tatttgttct aaaaattcta
     3421 ccatcttttc ttctaatcct acctatatga gttattattg ctccagattt agactttaat
     3481 tctgatttta attcttttct ataatcatat cgcttgtcta tagttacaac ataatcattt
     3541 atatcaaatt caattctacc tgcgtatacc ttatcattgt atttaattag ttttccaggt
     3601 attttatcta agtttattat attaaaatct acatattcaa cataggagtt cttagatttt
     3661 atgcagtcat cattgatata accttctaca ctaaattcac ttaaagtatt tattgttata
     3721 gaaataattt tgtatccatg aacttcaaga atagcattat cataatccaa aactaaattg
     3781 tcatcttcat tgtctatctc ttcaacacaa cctatgtctg ctttaaaact tatagatatt
     3841 ggaggtgtca ttttataata aattttccca ttacatctat attttttatc taaatatatg
     3901 caaaatctac cttcatagat actaatatct tcattgatac catagcttaa atactctgat
     3961 gttatagcat cagcaatttc tttcattttt attcccacct tcgcttttat gtaaaactca
     4021 atatattata taatttaata tattattttc atcaattata tacctcaata aatttctata
     4081 aaaatagtat cattttagta tcatcattta tttaatacat tatgtattcc gtttagattt
     4141 agttatccga aaacttagat aactagcttg aaatttctac agattattac atactttatt
     4201 gtaattatat cataatgtta taattacata ttatcaaatt acacactatc ttttattcat
     4261 atagatatta tcatccttgt gtatatattg tcaaagaaaa catttaattt acaacatatt
     4321 ttatatcata tcatctaaat tattatatac accttatcta attcacacat taacactatt
     4381 gtactttttc taaaaatttt taaataaaaa tgacagcttt aatttattat tttaaaactg
     4441 ccattaaact gtaaatattc ccttttagta tacaaaattt tacctttatg ttaatatatt
     4501 ttacttatat aaatttttca taattaattt atacatataa attagcacca ttgttgtttt
     4561 attatcttag ttataaataa ttgtaactag ataatattca atttctatat taatatttaa
     4621 ctaatattag ataatgtatc gtttatatcc ttttctacta atggtttcat attattatta
     4681 taaatactta tatctgcatt acttaattcc atttttatgc gattaataag attttcttta
     4741 tactttgtaa tattaggtat ggatttaata aacattcttg atacagaagg ttttttatct
     4801 acgatatgac ttagtatact atctatattt atttcaattt tattatcaat atcccactga
     4861 gcatttgcag agattaataa caatcccctt gttcgaatat atgagttttc gttatcaatc
     4921 atttctagga atgagtcaaa aaaacaatat gtaatatttg attttaaact ttcattttct
     4981 aagaataaaa aagttttata tgcttcatta tgattttttg aatataattt attaataagt
     5041 aaattgagat tcttcaatct tctcatctcc ctcattacaa atatttattt aaacaaagta
     5101 taacatattc aaaaataaat ataaataaaa actatattaa ataaaaatcc taaaattatt
     5161 tgctgtttat ttacaaataa taattctaaa atatatttga tttatttagt atcgactata
     5221 aaaataagct atcaaataat ttatacttga tagcttattt tcttataatt ctttttttaa
     5281 agtttccttt agactatcat atgcgaaatc cattatttct ctacaagcaa ctaaatcttt
     5341 ttttaggtct atacataact ctgaaccata ctcttttctt actgttgtag ttaaatcttt
     5401 agcatatact ttagctttag tactttcatc ttcgtggttt ctgcctttta cgtatccaat
     5461 tattatattt gatgcattta ctgcaccaca taaactagca accccacacc ctgaacccag
     5521 tccacttcct aaacaaactg gtatatctgt attaaattcc tcattatatg cttttataac
     5581 tgattctgca caattaaatc ccttctcatg atattttgat gctataactt tatcacacat
     5641 taaaattccc cctcattttt gtaaattaaa ataaattctt cattgttttt gtaaaaaata
     5701 cttcttttta ctattattat atcaaatatt aacaataaaa acatttcttt ctaagttaac
     5761 tctgtctttc taaatttaat agtatctctt tcaaatcaga atgattcccc atatatccac
     5821 caatattttt tttgccaact actctatgac aaggtattat tattggaatt ttattacttt
     5881 tacttgcttg tcctattgct cttactgatt ttggattgtt tatatttctt gctatatctg
     5941 aataacacaa tgtttctcca taaggtattt ttaacatttc cctataaact gatttttgaa
     6001 attcagtccc ttcaatacga atatttaaat taaagacttt tctttttcca ttaaagtaat
     6061 catctatctg gcttattgct tcctttaaat cttccttact tatctttata tcaattcctt
     6121 ttttaataca ttcatttttt aaattattaa atttttcatc tcctatgtat acactaacta
     6181 taaaaccatc ttcacttaat atgtacaaat ttcctaattt tgatttataa acatcatata
     6241 ctaaactcat attacttccc ccctttaata tcagtataca tataattttt taactaataa
     6301 agagttaccc taaaaaatag gataactctt ttttatagat acttaatata gagtatattt
     6361 ttaaattcat taacttttaa catatcaaaa tataaatctt atatattttt aaaagcctta
     6421 gaaagcataa gttttatcct gttaagttga tttacctcac ttgcagaagg gtcataatca
     6481 ataggtatta tatttgcatt tttatacaac ctctttaatt cttttataga acccttacca
     6541 attatgtgat taggtaaaca tccaaaaggt tgcatacaaa taatgttttc tacaccttca
     6601 tttaataatt caaccatctc acctgttaaa agccaacctt ctccagtttg attacctaat
     6661 gacactactg gttctgtact tttagcaaca ttttttatat tggctggagc ataaaatctc
     6721 tcactcttat caagtgcttt tttatatact ctttgatata ttccagttat gtatataaat
     6781 gcttctccta tcattctact ctttctactt ccttctaaat gagtgtgttt gtatatagta
     6841 ttaaaggcac aacttaagaa gaaatttgta aggtctggca cgactgcttc agcaccttct
     6901 cgctctaata tatctactaa attattattt gcaattggat ggaatttcac caatatttct
     6961 ccaaccaaac ctacttttgg ttttttgata tctagtatct ctaaattatc aaattctttt
     7021 actatatctt ttatatttct agtaaataca gaaatttttg ctttaacaag agaagatttg
     7081 catatatcaa cccatttttc atatagagca tttgcacttc cagtaacttt ttcatatggt
     7141 cttactctat atagtacttt catcagcaaa tcaccataaa caacagacat aaagagtcta
     7201 ttgattaatt ttagtgatat attatccatt ataccactat cttctattcc atttacacta
     7261 agtgcaatga caggaacatc tttaaatcca gcatcatata tagcctttct taagaatcca
     7321 atataatttg tagctctaca acctccacca gtctgagtta tagtaacaga agtattattt
     7381 aaatcatatt tgccagattt taatgctgtt attagctgac ctacaactat tatagctgga
     7441 taacatgcat cattatttac atatcttaac ccttcttcaa ttacactact atcagtatct
     7501 tttagaattt ctatattata accacttaag ttaacagctt tttcaaggaa ttggaaatgt
     7561 attggagaca tttgtggtgc taatatagta tgtttgttcg ttatctgatt atttttagca
     7621 tactgtatcc tgtttacctt tatatttttt acatctatat ttttgctttc tctctcaaac
     7681 atagctgctt ttaaggacct catccttatc ttagctgctc ctaaattgtt gccttcgtca
     7741 atctttataa tagtatatat cttcgatttt tcattaagta tttcttgaac ctggtctgta
     7801 gttactgcat ctaatccaca gccaaatgaa ttaagttgaa caagttccaa atagttttta
     7861 tctcttacaa aactagctgc tttatacaat cttgaatgat atacccattg gtctacaact
     7921 ctaagtggtc tttgtacatt ggctaaatga catatagagt cttcagtaag cactgccata
     7981 tctaatgaat ttataagctc tggcattcca tgatttattt ctgggtctat atgatatggt
     8041 cttccacata acacaatccc cttcatattt ctttctttta tttctttaag tgctctttca
     8101 cctgctgctt gtatctccat tttaaataaa ttctgttcat cagtagcttt atcaactgca
     8161 tgattaactt cttctttagt tatttttata tcagaaaaat gtcttgataa ttcatcatat
     8221 agtcttttct tcaatttttc cttatcattt agacttataa atggatttat ataatttatg
     8281 ttcttatttc ttatattctc tacattgttt tttattactt cagaatatga tattacaaca
     8341 ggacaattat aataattatc tgcatttgaa tcctctttat tttcatttgt tacacttgga
     8401 taaaatatat tttttatacc ttttgcaatt aaattttcta tatgtccatg aactaatttt
     8461 cctggataac aaactgtttc agatgcaata ctagttatac cagtttcata taactgtttt
     8521 gatgacctat ctgaaagtac aactctaaag cccatagcat tgaaaaaagt aaaccaaaat
     8581 ggataatctt catacatatt taatactctt ggtatcccta tttccccatt ttttgcttct
     8641 tctttttcca atggtttgta tctaaaaatt ctattgtatt tgtacttaaa taaatttatt
     8701 ggttttttct tttctaatcc tattttttta tcaccataaa tagcttcacc cttctcacat
     8761 ctattacctg aaataaaaat ttcattatca gaaaatttat ttattgtaag aagacaatga
     8821 tttgaacaac ctttacatct agttacactt gcttctagct tgatattatt caactcttcc
     8881 ttagataaca aggatgtttt gtatccactt tcatattttt ctttagctat aagtgctgaa
     8941 ccaaaagctc ccataattcc tgaaatatta ggtctaatta cattttttcc aattagtttt
     9001 tcaaagcttc taagcaccaa atcattataa aaggttccac cttgtacaac aatattatta
     9061 ccaatctcat ttaaatctct aatctttatt actttaaata aagcattttt tattactgaa
     9121 taagatagcc ctgctgatat atctccaaca cttgcacctt ctttttgagc ttgttttact
     9181 ctagaattca taaatacagt acatcttgaa cctaaatcta caggattttg agaataaata
     9241 ccttcatgtg caaattcttc tattgtcata gataaagaac ttgcaaatgt ctctaaaaat
     9301 gagccacaac ctgatgaaca agcctcattt aatattatat tatcaattac tccatctttt
     9361 atttttaggc acttcatatc ttgaccacca atatcaagta taaaatctac atctttattg
     9421 aaaaatttag cagctttgta atgtgctata gtctctatct ctccgttatc gattttaaga
     9481 gcttttttaa taagaccttc tccatatcca gttacagttg aactcaatat tttaatattt
     9541 tttggtaata tatcatatac ttcatttata actttaatgg ttgtttttaa tggatttcct
     9601 tcattgctat tataataaga atatacaagt cttccatcct catcaataag tgctgcttta
     9661 gttgtagttg agcctgcatc aattcctaaa taacaattgc tttttattga atttatgtcc
     9721 acataactta ttttttcttt ttcatgtctt cttgaaaatt cttcatactc atttttatct
     9781 ttaaataaag gttctaataa atttacttct ccatcttcaa tattctttat actatccaat
     9841 ttttctatta gagattttag ttgtatatta tcttcatcta ctgataataa tcctgctcca
     9901 atagcaatat aaagttgtgc attttctggg aagattatgt tttcatcttt taaatccaat
     9961 actcttttaa atgcttctct aagttcagat aaaaagtaca atggaccacc caaaaacgca
    10021 acatttcctt ctatttttct tccacaagaa aggacactca cagtttgtac gacaactgca
    10081 ttgaaaatac tcattgctat atcagtctga ttggctccat catttataag aggttgaata
    10141 tctgttttag caaaaacacc acatcttgaa gctataggat atataacatt atacccttta
    10201 gcaagttcat ttaatccact ggcatctgtc tttaaaagtg atgccatttg gtctatgaat
    10261 gctccagtgc cacctgcaca tattccattc attctttggt ctattcctcc acttagatat
    10321 gttatctttg catcttcacc cccaagttct atgacaacat cagtttcagg atgaaaatac
    10381 tctattgatt ttgtactaga tataacttcc tgaacaaatt ttacacctaa tttttttgat
    10441 attccaattc cacctgaacc agttattata atggtagtat ttatatctcc caatttttca
    10501 tatataccat ttagtacttc tttaactgat ttttttacat cagaataatg tcttctatat
    10561 tctttatgaa ctatattatt actattatta agtaccacaa ccttcaccgt tgtagaacct
    10621 acatcaattc ctatatgaaa taattcatcc attgtacccc tccatattta tatgcttttt
    10681 taatttttgt aagttctatg gctttatttt aagtaagtta tttataatct ttatgactaa
    10741 tatttttatt ttatgattct tttattaatc aaagcttact ctactaggta attcctaatt
    10801 ctaaccttaa attttaaata agaacatata gttagtgtac taaattttgt cataaatttc
    10861 tacagatttt tgttactttt tacataaata taacttaagt ctaatatcca cttaatgttt
    10921 atgtattata aattatcttt agtcagaaaa aaatggttgt tgatatgtaa attcttatta
    10981 catatcaaca accatttttt tgtatattat tttttatctt agctgattac gtttatccta
    11041 cgagtgtaaa tataaagtga gctgctgctc ctgcacataa accacctatt gtatgagaaa
    11101 gaatagcttt acctgccaac tgtctagcat ttaatgcatc catcatacca atatgtgtac
    11161 ttaaatatcc actccaacac atacccatag ctgtaaatac tgctatatca ttaggagtta
    11221 tagctcctga ctttataaac tctggaacta gagacattgc tgcaccaaca gctcccaatg
    11281 aagttatagg gaatgctatt gcctctggtg atgtaaatcc aaataatggt tctaatataa
    11341 atccaagttt atctccaata actggaagta attttattcc ttcatatgca gctcctgtat
    11401 aaacttcttg acctgtaaca gggtctgtag atggtccaaa agttaataac atcaccaatg
    11461 tacatactac taggactcca ggaattatag ccattcccat atctaccccc attttaccac
    11521 cttcaagaat tgcatctaaa gctctttgaa atacatttcc ttctctgatt tctctaaatt
    11581 catccttttt ctctaatgtt cctgttgctt tatcttcttc cggattgtat ttataaaact
    11641 tcttagtaaa tgtcaacatt attctaacac ttatgacgct acctataatt gcacctaaat
    11701 taccaattaa tgctggaagt acatattctg taccttgaga aatcataaat gttgttaaga
    11761 taagtcccat accaaaggct gttcctaagt tacataatgc tggaacttgg tattgtttaa
    11821 aatactgtgt gaatgtttta tctttagcaa aaggtataat agctggatta tctgataaat
    11881 aagttgttat agctcctgct atactagcac caggtagtcc atataaaggc ttcataaata
    11941 cagcgaaaat tttattaatt aacgctatta ctccaaactc tgaaagtagt gcgcttaatg
    12001 cgcctgcaag aactgccata gccataatta agaaaactgt ctccaatagt aaagcatgtg
    12061 ctgtactcat tattactttg aacatcattc ctgctcccat aatactacca acatacccaa
    12121 aaccaactac tagtaatatt aggaaaatga aggtttccat gcttactgcc ttaacctgtc
    12181 tcctgtctgt taacttctcc attttgaatc atcccttctt tttttgttat aaagtttaac
    12241 aaaacctttt ctctttttta attatactta taagtattac tatttgcaac tttatttgtc
    12301 atttttttat catttttaaa atgaaaatca gtattttaat aataaaaatt tttcagattt
    12361 atgcaagttt tcgacatctt atctttcatt ttataaaaag tatgattcct tactaaatat
    12421 atatatactt ctctaacgac ctaatatgca aatataagtt gtaacttgta tctttttgca
    12481 tgaaagaaaa aagattatga aaaaattttt ccttcaatat ttttcataat cttttttttt
    12541 agttttatat aattttttca aaaaattttt ttaaaatttg ctttaagaaa tatatattat
    12601 tttaattttt cagctacata ttttactaag tcaacagttc ttgaagaata tccccattca
    12661 ttatcatacc aagaaactac cttaaccata ttgtcttcta taaccattgt ggatagagcg
    12721 tctataatag atgatcttga atctccttta taatctatag aaacaagtgg cttgtcacaa
    12781 tatcctaaaa caccttttaa ttctccttca gcagcatctt ttaacacaga gttaacttgc
    12841 tcagatgtaa cgttttcttt aagttcgcac actaaatcaa caagtgaaac tgttggtgtt
    12901 ggtactctaa gtgaaaaacc atttaatttt ccttctaatt gtggtagtac tcttgacaca
    12961 gcttttgctg ctcctgtagt ggtaggtatt atagattcag ccgctgctct tgctcttctc
    13021 aaatctttat ggcttttatc taatattctt tgatcgttag tatatgaatg tactgtagtc
    13081 attaaccctt ttactatacc aaatttttcg tccaatactt tagcaaaagg agctagacaa
    13141 tttgtagtac aagatgcatt tgaaattata ttgtgctttt gattatcata ttctttctca
    13201 ttaactccga taactatagt tatatcttca ttttttcctg gagctgttat aattactttt
    13261 tttgctcctg ctttaatatg tttttctgcc ttttctcttt gtgtaaataa tcctgttgat
    13321 tctattacta tatcaacccc cagttctttc catggtaatt cttctgggtc actatatctt
    13381 aatattttta tctctttaga gtttactact agagtgtctt catcctttgc ctctacttca
    13441 cctctaaaag ttccatagca tgaatcatat gtaaataaat gtgccaaaga ttctgttgtt
    13501 gctcttgcat ttatggctac aatttctata ttatctccta attcttcttg agctattctt
    13561 aatacagctc ttcctattct tccaaatccg ttcattccta ccttaatttt cattatgaaa
    13621 tgtctccctc cattactatg tttttacatg attattgtac actaaataaa tcatttgttc
    13681 aatacttttt cttgattaaa taatcaaatt ccatgttttt ttattatcaa aagacaatta
    13741 gtatatcatt tttctttatt tcacccctca tttagtatgt actttttagt gtaaataagc
    13801 tgaaatcatt tattttactc aatgtccctt gggacatatt ttgccatctt ttttttgaat
    13861 ttttaaacta tacttatttt taaaaataat atatcttcaa gaatctcctc taatttaaaa
    13921 taataaaaac aaatttttat ttagttcaaa aaatatatga ttttttaaaa tttgccacaa
    13981 ttattattta tatatcaaaa tataaataat aattagctta ttgttaacta aattatggaa
    14041 atatacttaa ttgaaaactt aaatttattt ccttgtgtta tcaagatttc atgcttacca
    14101 tttccaatat catgctcacc aacttttgat gtgcataaac tatttcaagt aaaaatgcac
    14161 aacctatcct ttttgatagg aaaaagcagt caatcataaa tatcaactgc ttttatgtac
    14221 tgaatatcaa gttatagaca agctggaatt tattttgagg gtttttcttt ctccttaact
    14281 aaattgcttt caaaagtaac ctttacaact gcatgattta ttttaattcc ggtaaagcat
    14341 atagcatact ctttctcttt ttgtatatta gaaagagaaa ttgcgaatgt atcattatca
    14401 acgcttccat gccatgtatt actccataaa gtttcatctg ttttattgtc ctttatttcc
    14461 acaataccac tatctccatc cattttaaaa gaaatctctg tgtctaaaac atctatatca
    14521 ttagacacac aaaacaaacg tgcattaaca aatgggtctg aagtatcata atttttatct
    14581 aagtccattt caatggttgt ttgtcctgat tctttgttgt ttaaattatt atatttactt
    14641 tcattgcttt ttttacaagc tgtcaagcta accacactaa tgatactaat taaaataata
    14701 actaattttt tcatagaatt tatctccttt acaatttcta atttttaacc ctacaaacta
    14761 taatattaaa ctaatctact attttacatc aaatttttca atcattcagc ctttaccttt
    14821 tctcatttta attgcaaata tgctaaataa ttttggaatt ttaattattg tagtatctta
    14881 aattggttta taacatatta tgaaaattta agagtcaagt aacttattta aaaatacaca
    14941 aaattatact ttattgtata aaaaaagaga ttataattta tacaagtaaa ataaacttta
    15001 ttattttact cttctatata acctcttttc tatatatcta ttcaaataat ttagctccta
    15061 aactcacaaa catttgtaaa atcaaaaatg cttttgtgtc tatcttatga ttactttgta
    15121 aaatctcttt tgcatcttct tgcgaaatta aaagcgcttc aatctcttca tctggttcta
    15181 aaaattcatc tgttatatca ccatcacata tacaataaac aaaggcaaca gattcatcac
    15241 tcattccagg agataaataa actttatcaa tacttttatt ttttttaatt tctaaaagag
    15301 taagtcctgt ttcttctcta agttctcttt caacagaaat atctatagac tctccttcat
    15361 caacaagacc tgctggtaac tcgtatatat atccattaat aggtactcta aattgtctta
    15421 ttaaaattaa ttttttacta cttttatgaa gcccaactat tacaactgca tctacactat
    15481 cttcttcatt cttcaaataa atatttttta attgctcact actttttctt gatgctacca
    15541 tccaaacctt atcttcatct aatttgtttt tatactctat ttcaaaaagt cctataaact
    15601 tattttcaat cattggattt acattcttta ttatagtttt tttcaaattt actacgacct
    15661 ttctttttta ataccttttt aacatcatta agtaaatata atatcttaac aagtattcaa
    15721 caacttatta tatttccatt tcaggattat ctttatctac aaaacctact ttgatttcac
    15781 aattaaatat ttcctttaac aattttgcta attcttctct tgcactcatt accttatcta
    15841 aattagcatc agtaaaacca tctataggaa aaaatatatt ttttgagcta ttagtaattt
    15901 ttccttcctc tccttgtctc catatccatc ttctagtttt taccatgtct ccaactgaat
    15961 aaattaattc tccttcttcc attaattcaa catcagtttc tccaaatggt acaaatttat
    16021 cccctttttt agaaaatcta acatatacat catcatttct aaaatccata tcatgagcac
    16081 ccattggaag taaatatttt aaagaaatag agtttcctaa atctacaata ggatttatat
    16141 gaggaagttt cttattctta gcaacccttg ttgtcattgc ttctatcgaa ctcataaatt
    16201 tatttgggtt catgcctaga cttctaaatg cctctctata tggtataatt tcttctgatt
    16261 cctttacttt tttatctttg aagtaatctt ctactctatc tatacttata tctaaaaggt
    16321 tgcttattct ttctatttct ttagtattat ctattccttt tgcaactact acaccaaagc
    16381 atacattttc taatttatca aaaacttctt tttctactat aaatttcatt ttgctttatc
    16441 ctcctgttat tttacattat tttctaaata gattttatca ctttgtgtct tataaatata
    16501 ccccctattt gctaattgtt taattttttt aaattttcca attaatatta tatttattct
    16561 tatcagatat tttatatgta ctaattacaa atttaacttt ccaaaacaaa tatcttgttt
    16621 tataatgtat aatatatttt tatggaggca agactgtgaa aaattttatt ctaatatatt
    16681 ttatagtgat taattctatt gctttctttt caatgtacat agataagaaa agagcaatta
    16741 gaaacgaatg gagaataaaa gaagctactc ttatgtctat agcagtaatt ggaggtagta
    16801 taggttctat gattggaatg tattctttta gacataaaac taaacacatt aaatttacat
    16861 ttggtatacc atttatacta tttttacaat ttttattagt atatttttat attttaaagt
    16921 aattgaaata taaaacctta ttcttttcaa taaattaagg tgtataaaat taaaaacttt
    16981 atacaccttt ttctagcaat tattaaaaag atatttaatt aaaattaatg acccttatgt
    17041 ttagcttttc tcttttcttg tttcaaatta aatattcttt ctttttcttc ttcttttcta
    17101 tttttagcta ggttttttct atcctgtttt ctttcttcat attgaagctt aagagcattt
    17161 tgagccttag tccctatagt ttctttttct tgttcctttt ttacttttct ctgcattctt
    17221 ttgtatccaa tgttgtcttt ggtcacattt ttttctaatt taatgctacc aaaatctaat
    17281 gagaaaaatc tttcaagtat aaattcatac acttctactt ctttaggttc tgctccaaaa
    17341 actaccctac atgcttcata ttttttacca tcttgtcttt caaatatacc tacccaaaat
    17401 ggttcttcaa ataagactgt taattttcca ctaattttgt ccatcgctag ttcctcctta
    17461 aattactttc taaagaacgg acaaccaagg aggcaggtta ctgacagcta ttgctgcgtc
    17521 tggactacca accagactgt gtttttatct ttatagatta aatattatct tttttaatca
    17581 tgcttgtcaa tcttagtatc aacatctatc atattgattt aagtgaaatc aagcttttta
    17641 actctacttt attattgtta acactaagaa aattaattat ttttcataat aaattagtta
    17701 ttatacttat ttaaatatca atctttattg ttccctgcat aaacaagact gcttcaccag
    17761 aaatttttac agtattatcc ttcaacactt cacactgaat tataccacca cgattagaaa
    17821 gttgagcagc taccattttg tgttttccca acttttcaga ccagtatgga ataaaattac
    17881 aatgtgatga accagtaaca gggtcttctg aatctaattc tggacaaaaa tatcttgata
    17941 caaaatctgt attacttcct tgtggtgtga ttattatacc taaccagtct gtcaatttac
    18001 gcaattgtgc gtaattaggt ttataattta taacttcttg ttcactgttt agcaataaga
    18061 ttaaatctct tgaagaatag acagcacttg gtacacagcc tatcaaatta gcttgttatg
    18121 gtgataactc tatttcaata ggcattattt ctggaaatat catttcatat agatttccat
    18181 ttcgtgtaac ttcaagcttt ccactttgag taaagaaatc tatccttttt acattaacat
    18241 caataaaatt tgatattaca taagctgctg ctaaagttgc atgaccacat aaatcaattt
    18301 ctgctttagg agtaaaccat cgtaattcat aatttccttt acctttaact acaaatgctg
    18361 tttctggtaa attgttttct tctgctattt tctgcattaa ctctaaaggt attattctgt
    18421 caagaacaca aactccagca ggatttcctt taaatagttt agttgcaaat gaatcaacta
    18481 tgtagtattc cattatttga cctctctttc caacataaaa tatacatcaa tttacattca
    18541 ataaaacttt aaatttctta catatatttt ttaatattgc tctgctcaac aactttttta
    18601 cctaacaatg taattgcttt ttttgcacaa ttatttacac aacgataaca cattgtacat
    18661 tgcccatttg caacaatcat acaattttta attactagat tcttcattgg acataaatta
    18721 acacatttac cacatacaat acattcctca gtattaattt ttagtttatc tgtatacata
    18781 tttgttttat tgataaaata caatcgttgt ccaaataacc ctacaatatg actcaaaaaa
    18841 cctaaccctt cttttggtgg tgacccatgt ttcagttttt tagcagcttt agttactttc
    18901 ttttctgctt ttattacaag ctttttatta tactctaaag aatgttttaa taccttttca
    18961 tctcctatac tatctggcat cttcacatgt aatccaccaa cagttatagc accgtatttt
    19021 tttaatattc tagctaacac accagagcca tctccactaa atagagccat agttgcaata
    19081 ataaaaacac gtttaccatt ccacaaagtt gaatgattta ttataaaatc tttcaatatt
    19141 tttggcacat tactatattg cacaggataa ctaaaaataa tattctcatg attattgatg
    19201 tgctgtaaaa gttcattatc ttcaatagaa taagaatttg ctgttatatc atattcttgt
    19261 aaaaattttt ctacacagta tcttgaattt cctgttccac taaaatatat tcctatcata
    19321 gtacttctcc taatagctaa gattatatag ctctattctt aaaattctga ctttttatat
    19381 tatctaaatt aagtagttta tttaaataat ataaacttaa ttcaacaagt tacctactga
    19441 acctcccatg actaaagtca tagggttcct gcttgataga attttacata atcaatttac
    19501 taaatacttg tctaattctt tttttatcat tttaaatata cattttaata atattagtaa
    19561 gctatacttc cttaagcata tattttccct aaagtaaaac tattgatgat tattccatta
    19621 tataacctaa ttataaattt agtaagttca tcacatacta tacccttaat tctcaattat
    19681 aaagttccct aaaaaatatt tgaaaattta actatgaaaa gatttcctat tgcctaatat
    19741 gtataagtaa ggagtactat atagtactcc ttatattgta ctcttataac attgatacct
    19801 taatattatt ttaatcttaa tcattattga tttgatttaa aattataaat aggttccaat
    19861 attttaatta tgtcaactat atctcttata gttttaataa tctcatcaat tggtttatgt
    19921 gcttgttgca ctttatcaat agtattaatt aaatccattt acacatctag cacctgtatg
    19981 gcaatttagc attatcctta tcttatactt tgttgtaaat tcttgattac ataattctat
    20041 tatctaactt atagctgtgc aatctacatt atctgtaaat atctttgctt tatatatttt
    20101 ccctgaacct ctaacattaa tataacagcc tttttgctat acacttttaa ctatatactt
    20161 aattttaata acattacttg tttataaaaa tatactatag ataaatttca cataaaaata
    20221 ataattcaag tattgatttt tatataagaa aatttgtaat gtactaaaaa taacataaaa
    20281 gtttccattt ttcaatatgt aagcttttct tatgttattt tattgttaaa ggtttcaagt
    20341 ttttatttat tcattttaaa aaaattatta tttttgtaaa actaatccca tagtcatact
    20401 atcgtcagaa tatgcttcac ccttaacatt actaaaaaac tcaacagact taaatccaat
    20461 attttcaact tctaataaga aactttcttt tgaaaaatag tgattccaaa cataaaatac
    20521 attttcatct ttttcggtta ttacaagagt ttgttctaat gtattataat cattgtattt
    20581 acaattatct tgtagacaca tatatttttc attactccaa aatcctccat cttcatttat
    20641 ttcccaatat ttaatttcct caaaattatt gtatttattt attgtaaata catctaatat
    20701 taattttcct ccaggtttta aactatcata aattttttcc attaataacc ttctattttc
    20761 agttgacaaa gctccataat cacaataaat taaagtagcc aaatcaaatt catcattata
    20821 gttcatattt aaataacttt gaaacaagta atttatgttt aaattagtct ccttatttct
    20881 attttgtgca tagttaatag aacgttttga gaaatcaatt ccagttacct tataaccttt
    20941 ttgtgcaaat ttctctgcat ataaacctgg accacaacct aaatcaagta aattaggata
    21001 attaactgga tttgcaactg taactatcca atttacagaa tcctcgataa agttaaaatt
    21061 tctacttgct ccttcaaaat tagtgtctaa atgtgctttt aataattgtt ttgaaatata
    21121 ctcatcatcc caaaaattaa tttcactttg tttataaagc tctggtcttt ctaaatacat
    21181 acataatttg tttaacaaaa cataccactc cttaaaattt atttttaaaa taaaaaaacc
    21241 acagagttta ctctgtggtt taacttcaaa aagatgagtt taaataagag taaaatagaa
    21301 ccaaatattt ttaagcacaa caataaaact atagctttac tttgtttgct tattaaattt
    21361 gattttattc actcttttcc agcaatactt atatcaacga ttttatgata tcaaatatca
    21421 ccgaacaaca tcatcctttc atatttttat tttatgaatc aattatatat ttaaatttta
    21481 tatttgtcaa taaattttaa ttttaataaa ttttaatcat catagtaaaa aaaaattttt
    21541 tatataaaac taatcaaaaa aagatatctg tccatctcct gagtttttta acttttcctt
    21601 cattatttta gagtaactgc ttgatagaga atatttatat ctaagttcat taaccatttt
    21661 atacaattga tttttatact ccctattaag tgaacctgca ctatatagta ttgataattt
    21721 atcaaataat tcaggaaact cttttttaac aaagtcaaaa aaaacacctc tagttatacc
    21781 ccttaagtat aaagtcccag gtaaaacata atgtacatta cattcttttg catgtctaaa
    21841 tagcgaattt atattatcaa aatcatctgt aatatatggt ataattggca taatatgtag
    21901 accaactgat gcgttagttt ttctaaattc ttttaacatt ttaaatcgtc tcattgaatc
    21961 cacaccattt ggctcaataa gcctttgtgt atcttcattt actgtggtaa ttgtagatgc
    22021 tacattgata tatgtaatcc tagatagctt atcaattaaa tcgtaatctc ttaaaattaa
    22081 atctgacttt gtagaaataa tagctggtgt cttgtatttt atcagcaggt ttaatatttc
    22141 tggcataatt ttatagtcag cttctattgg ttgataacta tctgtaactc ctcctatatt
    22201 gattacttct ttcttccatt tactgctttt aagttgtctc tcaagttttt ctactatatt
    22261 tgtttttaca taaatctctt caaaataatt attggagtta atgtacttat gagagtaaat
    22321 agcataacaa tatttacatc catgctcaca tccacgataa atatttaaat cccatttata
    22381 tggcatactc ctttttaatt tattacaagc tatttcacaa tttattggaa tatattttat
    22441 tttattatac aaataatcac ctctttatat acaataatac tataatgtta atctatagta
    22501 attaaaacat gatatttcca gaccacaatt cataatcttt actatattta caaagtaata
    22561 tattccattc atttattggt attcctccac atgttgcttt ttcttctaaa ttatataagc
    22621 attgctttaa atacatttta ttatgccaat catcatataa attttctaaa tttaaactat
    22681 gttcattaga tatatcattt gcaaaatttc tatttttcca ttttaatata cttgtatatt
    22741 taagttcatt aggtttataa tttcttttta acatttcatc atgtacaatt tttgtataaa
    22801 atttaaattc atctatacta taattcaata ctttattcac taatagatgg tttggagtac
    22861 cttttttatc tatagaacct tttatagcaa gtagctctct ccattgactt actaattgat
    22921 ttttaggtaa tacatcaatt aaatccttat gccataatct cataatttaa ctccttacta
    22981 taaaatattt tataaattca ataaccaatc tatcttacaa ttttacatgt tcaataattt
    23041 tatctgtaaa aactatacca tttaaatggt ctatctcatg acaaagacat tttgccataa
    23101 aacctgaacc tttaatttct attttctcac cgtattcgtt tagagcttgc actgtaactt
    23161 tttttggtct ttttaattta ccccatactt ctggaaaact taaacatcct tcaacaacaa
    23221 tttgttcccc ttcttgttta ataatttcag gattaacaag cttaataagt ccttctccta
    23281 aatctataac aactaatcgc tttaatactc ctacttggca tgcagctaat cctcctccat
    23341 ttggagtatt gtacatggtc tctgccatgt catttaatat ttctcttatc ttattatcta
    23401 cgttttctac atacttactc ttttttctta aaatttcatc atcaaatgtt cttatctttc
    23461 ttatagccat aatattctcc cctacttatc tgtgctcttg attttctttc actttcttca
    23521 aataaaaata cactttcaat tggttcatta aaaattcttg aaatatcata tgccaaccat
    23581 attgaaggtt catttttacc agtttctata gcatttatag cctgtctaga tgttccaact
    23641 aatcttccta gttgttcttg tgtcaatcct aatttttctc ttaatatctt tattttattt
    23701 ttcatatttg ctccattcat gtaaggttaa ctttacatct taaatgtaac atttttcaac
    23761 attactgtca agtcaacctt acatttatat attaaatttt cttatttaac tattttttta
    23821 ctaaactttt taaataataa taaaatccat attaatgata taacaattga aataaacata
    23881 tagatataat tatcttcctt aaatgccttc gatatccaaa aagttggtaa gaagaacaga
    23941 atatattgtg ctttatttaa aataaaaaat ggtgctaaaa tacccatcat aaaaacacct
    24001 gagaatttcg taattaccat tccttctagt ttatttgatg ataaagaaat cactagtatt
    24061 gatataataa ctccttgtaa taaaactaat aaagaaattg ataagtttaa caagaaagaa
    24121 attttagtta gagaaaaaaa gataagtaga atcatagtaa taataaatgc taaggtacct
    24181 ggtattccaa atcgtgaaat aagataacca gttttaccaa gcggagttac tattaaatat
    24241 cttgaaatgc tgtcatcaat ttctcccaat atgaccattg tagaagcaaa aaagaacata
    24301 aaaggtgtaa taaaagctaa aagtaaatca aacatcaaga aatacggctc taaatttaat
    24361 tgataataaa acttgttaag tgatatattc tgtattaatg gtattccaaa ttttataaat
    24421 actccacata atattggaga aacacatact ataaaaagca tagcatctct cttaatttgt
    24481 tcaaactctt gtttaaacgc atttataatc tttatcatag ttttactcca cctacctttt
    24541 tccaactgct acaaataaaa taataagtta taaaataaat tattattata attgatatta
    24601 aaatattaat cattataaaa tttttatctc cactaataag agaaatacat atattaaaag
    24661 ggtacaaatt tgcatatgat tttgtgtcaa gaagtacatt gagtattggt ggaataaagc
    24721 atataatctc aataggaata cttaaaacaa taaactgatt caaactagat gctttacttg
    24781 caagtatcaa tccaagcaaa gaaaatataa tagaagaaaa aaaggtacct atagttgata
    24841 ttataatatt atctaaattt aatgtaatag caataaacat agctacaata ctagatatta
    24901 ctcctaatga aattaccttt gataaaatat actcagatac ttttactgga gatacagcta
    24961 aggaatttaa aaccctttga cttttctcaa gaagtacaat agctcccata aaaaacaatc
    25021 ccattgctgc tgggtcagaa taaatcataa ttatagctat tttttctctc ataaaagttg
    25081 gaaatacatt gataatacaa atatatataa tacttaaaaa aagatataaa aaataaaaac
    25141 catacttaaa ttgaaacttt atatctccta aaataagatt ttttagcctc attgtaaatt
    25201 cctccctgtt atctcaataa aaatatcatt taatgttggt tcactactat gtattgataa
    25261 aagtttattt ttcttaataa gcatattcaa atttttatca ttagcagtat tgttcagaaa
    25321 acattcagaa gttttttctc cattatcaaa gtaagtatat ctaactttaa tagctccttt
    25381 tgacataata agattatgtg gtgtatctaa agcagatatt ttaccattta caatgaacgc
    25441 tactctgtca caaagctccg ttgcatctaa catgttatga gttgttaaaa taatagtttt
    25501 acctttagat ttttctgata aaattatatc tttcataacc ttgctatttg acgggtcaag
    25561 accacttgtt ggttcatcta aaaataaaat atctggatta tgtaataaag cttttataaa
    25621 atttaatcga gatttcatac cttttgaata ctcagatacc cttttatttg cttcattttc
    25681 tagtcctact gattttaata actcatcaat agatagtagt tttttagaat atagagaccc
    25741 aaaatatttt aaattttcta ttgcagtcaa cttttcatat aaactaggaa attcaaaatc
    25801 aactcctata ttttcataaa atttatttga gtgtctttta ctctctactc catttacaat
    25861 gacacttcct ccataattag ttatcattcc aattagaatc ttttgtaatg tagattttcc
    25921 tgcacctgat ggtcctaaaa atcctagtat ttcccctttt tctacttcaa agttaatatt
    25981 ttgtaaaaaa tttctatctg tataactaaa tgataaatca tcaactttaa tcattcaata
    26041 tcaccctttc aaatttttga ctattttgac tatatggtca ataaaatata atttttttgt
    26101 ttgttaataa tttttaataa aagttattag caaacaaaat ttaaattcta ttccataagt
    26161 tgtataacta atccgtttat cattaatctt aaagcatctt caaaacaatt acttccaacc
    26221 tcgcgcttat atagcatggt aagaaaaact actctaaatg ctccactaaa gctttctatg
    26281 tctttatttt ttgcatttgg tatgcaagat ataatctctc caatttcaaa gtcatcatgt
    26341 ctaaagtgtt cttcaattat agaatctggt aactttctca ccaatatctc aaattctcca
    26401 tttatcatga gatttaaaat tgaagtacta tcaaccatcc tatagaattt cataaatata
    26461 tctgtcaatt gttcacatgt aatactttca tctactctat ttatctcata taagatttct
    26521 ttttgtatag agtcatgtat atctctcaat acctcaaaaa agagcaattc ttttgattga
    26581 aaaaatagat aaaaagttcc ttttggtatt tttacccgtt ttacaagctc atcaacagta
    26641 gtttttctaa taccatattg ctccatacac aatcttgctt cttctttaag tctttttatt
    26701 atatactctc tttctttatc agaatacgat ttaggcatct ttttcctcca gtttgactaa
    26761 aataatttat atagtcaata taatactttt tctatctaat gtcaatagta ttattagtat
    26821 atttaaaaat aagcttaaaa actttcgaaa attctgaatt ttatatagca ttttttatta
    26881 tctagtgtta taattaagat ataaataata tattattcta attttcatat ctatttgctt
    26941 attttttaac ttagtttcta atagaagtac tcatatttat ctttttaaaa gtttcaactt
    27001 taataaaaaa taaaggagtt gattttatgg ctactactac aaaaaaccgt ttttacgatt
    27061 atgttcaaga aaacttaaat aaatatgttg aagtacattc ttactttagc agttgtcctc
    27121 tatatggtga aattatagaa gcagatagct tatcaattac actttgctca agatatatag
    27181 atgaatctaa tccaacttta aatgaaactt acacattata tttaccatta agttcaataa
    27241 tatctattag aacactataa aaagttaatt aaatatgggt tatttatata taaaatataa
    27301 gattttctat atttctatat atagtaaccc ttttttatgt ttttaaactt atactacata
    27361 aatttaaagc tgaaaacaaa actttacaat cattgattat cttatttatc aataatttaa
    27421 agtaattatt ttcagctt
//
LOCUS       DAEPOZ010000026        27097 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.026, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000026 DAEPOZ010000000
VERSION     DAEPOZ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27097
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.026"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            273..1607
                     /locus_tag="KR440_000909"
     CDS             273..1607
                     /locus_tag="KR440_000909"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HBH0697971.1"
                     /translation="MRVVIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFDDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREVSYQGSLSSSCIKIMD
                     MEAASTGITEKQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTSQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(1736..2653)
                     /locus_tag="KR440_000910"
     CDS             complement(1736..2653)
                     /locus_tag="KR440_000910"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBH0697972.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLNSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            2861..3745
                     /locus_tag="KR440_000911"
     CDS             2861..3745
                     /locus_tag="KR440_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HBH0697973.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEDKKVVEGSKKIQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRIKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGKKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            3958..5799
                     /locus_tag="KR440_000912"
     CDS             3958..5799
                     /locus_tag="KR440_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HBH0697974.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKNKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVEN
                     IKIENKSVRLIGKEEALKYKLDYVVSKKVDEIKEQQEKILDKVIGATTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGERITSIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPILKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSARESSNQGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
     gene            6103..6810
                     /locus_tag="KR440_000913"
     CDS             6103..6810
                     /locus_tag="KR440_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0697975.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            6804..8114
                     /locus_tag="KR440_000914"
     CDS             6804..8114
                     /locus_tag="KR440_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0697976.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNIAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMAQE
                     NYLKDTTESEETGYLVEYNEYFKEGTFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEKLEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKNEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            8231..8914
                     /locus_tag="KR440_000915"
     CDS             8231..8914
                     /locus_tag="KR440_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0697977.1"
                     /translation="MKILYTENLSKHYGKGENLVRALDNVNLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDESFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            8907..11294
                     /locus_tag="KR440_000916"
     CDS             8907..11294
                     /locus_tag="KR440_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBH0697978.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGEMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFELIKGKLPTKENEIVLDSGALKALGYKNKDLGEKIKISYNDYKNDK
                     KIEKEFVISGILKTSEISEAGKYYYAIISELYMRNTRDMSQEDFNIYVKFNDKSNLSI
                     EQAKEKLDKIANDIGLDTINTAVNENYINALKPDMETIMGGGFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQIKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKNNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNLIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKAVISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAMYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            11573..12715
                     /locus_tag="KR440_000917"
     CDS             11573..12715
                     /locus_tag="KR440_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBH0697979.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIVGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKEYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLDRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKIPESIGALKLTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNENLLNLFNKYYF"
     gene            12734..13258
                     /locus_tag="KR440_000918"
     CDS             12734..13258
                     /locus_tag="KR440_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBH0697980.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGSKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYITEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            13358..14176
                     /locus_tag="KR440_000919"
     CDS             13358..14176
                     /locus_tag="KR440_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH0697981.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYTASNMHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSELFAKKLEESLNPTQSVEKIVLYLEEMLLSILEEDLISNKSV
                     KLLNSFIYDNRYSNIKAFCDNTNINIKTLERICLKYTGYTPKILKRIYRFKMASSQLI
                     YNYKDNELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFIGENKTIKENIKYNYL"
     gene            14254..14565
                     /locus_tag="KR440_000920"
     CDS             14254..14565
                     /locus_tag="KR440_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBH0697982.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSASSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(14868..16871)
                     /locus_tag="KR440_000921"
     CDS             complement(14868..16871)
                     /locus_tag="KR440_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HBH0697983.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     NFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELIKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDNGI"
     gene            complement(17024..17662)
                     /gene="recX"
                     /locus_tag="KR440_000922"
     CDS             complement(17024..17662)
                     /gene="recX"
                     /locus_tag="KR440_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBH0697984.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKS
                     YNLVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
     gene            17938..18627
                     /locus_tag="KR440_000923"
     CDS             17938..18627
                     /locus_tag="KR440_000923"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBH0697985.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
     gene            18628..19200
                     /gene="yqeK"
                     /locus_tag="KR440_000924"
     CDS             18628..19200
                     /gene="yqeK"
                     /locus_tag="KR440_000924"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBH0697986.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSRNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            19317..19667
                     /gene="rsfS"
                     /locus_tag="KR440_000925"
     CDS             19317..19667
                     /gene="rsfS"
                     /locus_tag="KR440_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBH0697987.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            20021..22441
                     /locus_tag="KR440_000926"
     CDS             20021..22441
                     /locus_tag="KR440_000926"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBH0697988.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDTLDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            22868..23180
                     /locus_tag="KR440_000927"
                     /pseudo
     CDS             22868..23180
                     /locus_tag="KR440_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431045.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23279..23947
                     /locus_tag="KR440_000928"
     CDS             23279..23947
                     /locus_tag="KR440_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBH0697989.1"
                     /translation="METVSNNQSSVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFSEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(24105..25100)
                     /locus_tag="KR440_000929"
     CDS             complement(24105..25100)
                     /locus_tag="KR440_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBH0697990.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGIGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(25221..26849)
                     /gene="aspD"
                     /locus_tag="KR440_000930"
     CDS             complement(25221..26849)
                     /gene="aspD"
                     /locus_tag="KR440_000930"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HBH0697991.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDEI
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
BASE COUNT        10442 a         3125 c         4489 g         9041 t
ORIGIN      
        1 tcaatattat atcacaattt acttaaatag agtaaaataa attgattttg aaaaatgtga
       61 ttaagtatag aaaaataggg tataataaat gtttaaagaa tttgaattgt ggtaagaaaa
      121 taaaaaatag cttttataat atttgatttc aaattaaaat atgttaaata aataactaaa
      181 aattgttatg aaaaatgttt acatagaata tatatcaaat gaaaagctta aaataagtga
      241 agaaattaac tagacatcaa aggagagata aaatgagagt agtaataatt ggtggtgttg
      301 cagctggtat gagtgctgca gcaaaattaa aaagaataaa acctgagtat gaagttgttg
      361 tttatgaaaa aactgaaata gtatcttttg gtgcttgtgg attaccatac tttgttggtg
      421 gtttttttga tgatgctgat gaattattag caagaacacc agagaaactt agagaagctg
      481 gaattgattt aaatatattt agagaagtcg ttgaggttga tagtgaatct aagaaaataa
      541 aagtaaaaaa tataaaaaca gatgaaatat atgaagatta ctatgataaa ttgatgatag
      601 caactggagc aagaagtatt atgcctccta taaaaaacat aaaattgaag aatgtttcta
      661 cattaaaaag tttatatgat ggagaatatc taaaaaaact tttaagtaat gaagataata
      721 aaagagtgac aataatagga gctggtttta tagggttaga agcagttgag gcatgtaaga
      781 aattaggtaa agatgtagat gtaattcagt tagaagatag aatattgcca caagtatttg
      841 ataaggagat gacggatgta ttagaagaag aaataaatag acataatgtt aatttgcact
      901 tggatgaaat ggttgtagaa ctttgtggag aagataaagt agaaaaagtt ataacaaaca
      961 aaggggaaat agatacagat gtagtaataa ttgctactgg tgtgagacct aataccgagt
     1021 ttttgtcaaa tactaatata aaaatgctaa aaaatggagc tatagtagtt gatgaatatg
     1081 gaagaacttc tgtagaagat atatactctg ctggagattg tgcaactata aagaatatag
     1141 taagtaatga aaatgtatat gttcctttag ctactggagc taataaatta ggtaggatag
     1201 taggagaaaa cctagcagga agagaagtat cttatcaagg ttcactatct tcaagttgta
     1261 taaaaattat ggatatggag gcagcatcaa ctggaattac agaaaagcaa gctaaagatt
     1321 taggcataaa tgtcaaatct aaatttattt ctgattataa tcaaactcat tattatccag
     1381 gaagaaataa aatatatgta aaacttattt atgatgcaga tacaaaagtt attttaggcg
     1441 gacaagtggc tgggtttaag gatgctgtcc aaagagcaaa tgtattggca gctgctatct
     1501 ttggaaaaat gaccacaagt caacttggaa tgttagattt atgttatgcc ccaccatttg
     1561 caagaacatg ggatgtttta aatgtagctg gaaatgtgtc taaataatat ttagattata
     1621 gctaatatag ttgacaagca attaagggaa tgaatttacc taaactattt ttatcatggt
     1681 aaaattcatc cctaaatttt tattccaaat ttttagaaat aaggattcca ttaaattatt
     1741 catcaaaatt taaacgcaag cttaatctat cttctccaag aatagtattt tcaaaaacat
     1801 tagtagcatt ttctaaatac atactaggta tgtctaaaga aggactaaaa tgggtcagta
     1861 aaagttgttt gacatttcca agttttgcaa gattagcagc ttctctaaaa gtcatatgtt
     1921 tgtttctaac agcttttgat atatctaaat catcaccata catggcttca catataaata
     1981 aatcactatc ttttatgaaa tctggtattg attctatagg tcttgtatcg gttgtaagac
     2041 ttatcttaac tccctttctc aattctccta agaccatatc tggagtgtat tgttttgaat
     2101 ttaaaactat attttgacct tcttggagtt tgttccataa tattttagga acttcgtttt
     2161 tagttgcctt atctatatca aatttagggt ttcttctaaa attaaaactg tatcctaaac
     2221 actctataga gtgttctaaa ctgattgttg aaatttctaa atttcttaaa actttattat
     2281 ctaaagaaaa attgccttga gggttttcta taatttttaa agtgtatggt acatattcta
     2341 ctaaatttct catagaacgt atacaatcga ctattccaac tggaccaact atagttaaat
     2401 cacttgtacg accactattc ccaattgttg ataaaagtcc taaaagtcca aatatatggt
     2461 caccatgtaa atgggttata caaatcaagt ctatatcttt aaagccacat ttttttagct
     2521 tcatagatac ctgagtacct tcaccacagt caattagtat ttttctacct ttatagttta
     2581 taaatacaga ggatagaaat ctgtttggca taggtacatt tccaccacat ccaagtaaag
     2641 ttaaatctat cattttttcc cctttctaaa attaacacaa atctgttgtt tgtttttatt
     2701 atgatatcct attataatta ttttaacatt aaatataaca ataaatgtgt atttgagtca
     2761 agaaatatta tcaaataaag tttttatata gcaatgtata aaaatttgga gaacgaaaag
     2821 tgaaaattat atttaggagt atttaaagga ggaaaattta atgaaagtat taaagaaaat
     2881 tactattttg tcattgatta cattaacctt agttggttgt agtaacggag aagataaaaa
     2941 agtggttgaa ggttctaaga aaattcaaaa ttctaaaaaa gatgaacagc ctataatagt
     3001 acataaagat gcaaatatat tgaaagatag gtatgagcca ccaaaaggat ttaaaagagt
     3061 tgatgtagaa aaaggtagtt ttgaatcatt tcttaggaat actaagctaa aaaattatgg
     3121 agagaaagta aagtattatg atggaagaat taaggaatca aaaggtattt atgatagtgt
     3181 atttgatgtt gatattggca atagagattt gcatcaatgt gcagatgcga ttatgcttat
     3241 gagagcagaa tacttgtatc agaataaact atatgatgaa ataagttttg attttgttga
     3301 tggttttaag gcgaagtatt caaaatgggc acaaggatat agaatttcag taaaagatag
     3361 tggttcaagc tggtataaag caactgaata ttcaacatct tatgagagtt tcagaaagtt
     3421 tatggatata gtgtttgcat attcaggaac gttatctctt gaaaaagaat tggagcctgt
     3481 aaaagttgag gatatgaaaa taggagatgt gtttatagtt ggtggaagtc caggacatgc
     3541 aagtataatt atggatatgg cagaaaatga aaagactggt aaaaaggtat ttatgatagc
     3601 ccaaagctat atgcctgcac aacaaacgca acttcttatt aatcgtaaaa atccaaattt
     3661 gagtccttgg tatagtttag attttgaagg tgatttgaca atacctcaat ggacttttaa
     3721 aagagaccag ttgaaaagat tttaaaataa taattttata taataaaaga aaaatataga
     3781 tgtgaaaaaa tgtaattaga ttaaaactaa ttggatatgg acaaaattaa attgatagat
     3841 aaatattaaa aattacacta atcaatctta ctatagttta atatttatct aaaaagtttt
     3901 atgtaaaata taaattttat attaaagcta aaactaattt gaggaggcta atttatattg
     3961 aagattagaa ggtatttaat tttggtaatt ataattctaa ttacaacaag tcagagtttt
     4021 atttcttatg ggctagagcg atttgaagaa ataaccattt ttcacactaa tgatatacat
     4081 ggaagatatg ctgaaggtga tgaccatata caaataggaa atctagccac attgaaaaaa
     4141 gaaactccaa actcaatttt agtagatgca ggagattgtt tgcatggact tccaatagta
     4201 aatatggata gagggaaaag tgcaattgaa ttaattaaat gtgctggata tgactatatt
     4261 actcctggaa atcatgactt taattatgga aaagatagac tgtttgagtt atctaaaagt
     4321 gctaatttag gagagaatac tttaaaattt ttatcatcaa atatttttga aaatggaaaa
     4381 aaagtttttg atagtaatga tataaaagaa atagatggaa taaaaatagg attttttgga
     4441 ctctcaaccc aggaaacaaa gaacaaaact ggatttaaaa atgtggaagg attagagttt
     4501 aaaaatccta tagaaagtgc aacagaacaa gtaaatgaat taaaaggaaa aggtgccaat
     4561 gtaatagttg caatatgtca tattggtaca aatagtttaa gtaagccaaa tagtatagat
     4621 atagcaaatg aggttcaagg aattgatttg attattgatg gacacagcca cactaagttt
     4681 gaaaatggta aacaagtggg tgatacattg attgtaagtg caggacaata tttagaaaat
     4741 attggcgaga taaaactaac tttggatact aatgatgtag aaaatataaa aatagaaaat
     4801 aaatcagtta gattaattgg aaaagaagaa gcattaaagt ataagcttga ctacgttgta
     4861 agtaaaaagg ttgatgaaat aaaagaacaa caagaaaaaa tattagataa agttataggt
     4921 gcaactacaa atactctaga tggttcatat gaaaatgtaa ggacaaaaga aactaatttg
     4981 ggtaatttaa taagtgacat tttactagat aaaactaagg ctgatatttc tttatttaat
     5041 ggagggaata ttcgagatac gatagaaaaa ggcaatataa caagaagaga tattgtagat
     5101 gtatttcctt ttagcaatac tatagttacc aaagaactta caggagcaca gattaaagat
     5161 gtattggagc atggagtaaa actttatcca gaaaaaagtt cagccttttt gcaagtggga
     5221 ggaatttcat actactttaa tcctaaacaa aaagaaggcg aaagaataac aagtattcaa
     5281 aaagaaggaa agccattaga tttaaataaa aagtatgtag ttgcaacgaa tgattatata
     5341 gcctcaggtg gagatgaatt tccttgtttt tcaaaagagc caatactaaa ggaatttgga
     5401 aatttagaga gtgcagtcat tgaatatata gagtacaaaa aagaaatatc aaaaaatgta
     5461 gatggaagaa ttggtattaa atctaaagat gagttaataa gcaagaatca tgagagtgat
     5521 ttgatattaa gtgaaaatga aaaaaatatc aatgataatg aagagaatct atctaataat
     5581 ataaaaagct ctgatagtga caaaaataaa agcttagata aaacaagtgc tagagaaagt
     5641 tctaatcaag gaatatctaa aagagaagct aaagaaaaat ctcctaaaac aggagattta
     5701 ggtttttcaa atagtataat tatatttata gtttcaagta cattgatatg tttattgaat
     5761 tttaatcaaa aagagttaaa ggataaaaag tcaaaataaa aattaatgag caaaattagt
     5821 agatagtaat ttaattttag ataatagtaa acagcaaagg aaattagtta tataaatact
     5881 aatttccttt gttttatgct ttaacatgat tatttttaaa taaaattgta ttttatgaaa
     5941 aataattaca ctaaatggca tagtattatg gggattttta gtttttattc aagtaaatga
     6001 aatcatatta acaacttctg atatatttac aaattgataa aaaaatgatt ttattagtat
     6061 gatataattt agctagtata tatgatgatg gaggatatag ggatgaataa tatattactt
     6121 ttggaagatg acaaatcttt aaatagagga attagtttta aattaaaaaa agaaggatac
     6181 aatgttttta gtgcatttgg tatagaagaa gcaaaaaata ttttcgcaaa agaagagata
     6241 tgcttaatta tttccgatat aggtctacct gatggtagtg gttttgactt ttgtgaggag
     6301 attagaaaga aaagtgatgt ttacataata atgttgacag ctcttgatga agaggtggat
     6361 attgtcacag gatatgattt aggtgcagat gattatatca caaagccttt tagcttgatg
     6421 gtattaatat caaaagtgaa tgcgcttatg aaaagagtaa atacagtaaa aaattatact
     6481 ctattagttt gtgatgattt attcttttat tatatagaaa ataaattaat tataagaaca
     6541 gatgataagg aagaagagat aattttaagt aagacagaga caaaactatt aaaatatttg
     6601 atggaaaatt ctatgcagac attgactaaa gaacaactgt tagaatcttt atgggatagt
     6661 agtggtaatt ttgtagatga taatacgatt gcagtaaata taagaagact tagacaaaag
     6721 gttgaaaaaa atccatctga acctaaatat ataaaaacag taagaggggt tggatatatg
     6781 tggggagaaa ggagtataaa aaaatgctaa aaaatatgaa cctcaaagat ggtttaagtg
     6841 tgaaagatga ttatataatg agaagagtta ttgtatattc tgttttatct gtactaatag
     6901 taacccttgt atttatactt tttgaatata gcagattaca taaattgtat gaacatcaaa
     6961 taaaaaatac tcaaaatatt gctggaatat tgattgaaaa atatcctaat gatgaaatag
     7021 atatagtaaa ttctatttat aattccgatt atagtaaggt gtctactgga gaaaaggcat
     7081 ttaagaaatt tggctatgga ttggaaaata aaatgtcaaa tgataaaaat tttagtttat
     7141 acttaaaatc tttttttaag gaatcatttt ttattttttt aatgatgttt atagtagtta
     7201 taggtataat atattatttt ataagataca taaataaaag attgagtaaa atttatttta
     7261 tagtagataa gatggcacaa gaaaactact tgaaggacac tactgagtca gaagaaactg
     7321 gttatctagt agaatataat gagtatttta aagaaggtac atttagtaag ataaataact
     7381 gtctatatga gcttaataga agtttaaaga taaagtttat taaattagaa aaagaaaaag
     7441 aaagtgtaaa atcattagta acagatattt ctcatcaatt aaaaactcct ttagcatcct
     7501 taaaattata caatacatta cttatagaag aaaaattaga agaagatgaa gccaacgagt
     7561 tcttattaac caataaggct tctataaata aattagaaaa tttaataaat tctttagtga
     7621 acatatcaag gttagaaatt agcatgataa acataaaaaa agaagataat gatataaaaa
     7681 atactattct aaatgcagta aaaagtgtta cccctaaggc taagagcaaa agtataacat
     7741 taaatttaaa tgaatttaac agtagaatta taccacatga taaaaagtgg actgaggaga
     7801 gtatttttaa tgtattagat aatgctgtaa agtacacaca ggtaaatgga gaaataaata
     7861 tatctgtaga agaaactgct aatttcttta aaattataat agaggataat ggcataggga
     7921 ttgaaaaaaa tgagtttaat aatatattta aacgttttta tagaggttct gctgatgaaa
     7981 ttgaatctgt agaaggtagt ggggtaggat tgtatttaag tagaaagatt ttagaagaac
     8041 aaggtggaaa tattatagtt tcttcaaaag tagggatagg gagtaagttt tctttgtttc
     8101 ttacaactat gtaatattaa atgaaaggaa aatgtaagcc tctacattta ttatataagt
     8161 ataaacagct ccagaaataa aaaattaata aaatatgggg taatataact tagaatgagg
     8221 ggataatttt atgaaaatac tatatactga aaatttatca aagcattatg gaaaaggaga
     8281 aaacttagta agagctttgg acaatgttaa cttagaaata aatgaaggtg aatttgttgc
     8341 aataatagga aaatcaggtt ctggaaaaag tacactttta catatgattg gaggtcttga
     8401 tatacctaca tctgggaagg tgtatataga taataaaaat atatttacat taaaagaaga
     8461 agaactagct gtgtttagaa gaagaaaaat agggtttata tttcaaagct ataatttaat
     8521 tccatctctt aatgtatggg aaaatgtagt gttgccaatc ggtctagatg gtagagaagt
     8581 agatgaatct tttataaaag aacttttgaa atcattagga cttgaaaata aacatgacgt
     8641 tttaccaaat acattatcag gtggtcaaca acaaagggtg gcaatagcaa gagcattagc
     8701 tacaagacca gctataatac ttgctgatga accaactgga aacctagatt ctaaaacatc
     8761 tgatgaggtt atgagtatat taaaatctat gtctaagaag tacagtcaaa ctctagttat
     8821 gataactcat gatgacagta tagcccaaat ggctgataga gttatattca tagaagatgg
     8881 cagggtgtca aaggttggtg ataaaaatga ttaatactac taaaatagca aaaagtaatt
     8941 taaaacaaaa taaatcaaaa agtattctaa taataatcac tataatactt tctactttaa
     9001 tgctttcttc tattggaatt tatattgttg atgcaggagc atatcaaaaa gaaaatacta
     9061 taaagtattc aggtaattat caaggtatat tagcaaatgt agatgaaaaa caggctgata
     9121 tattgagtaa tcatgcagat gttgaattaa ctggtgaaat gaatggagta ggtgttgaaa
     9181 aactggaaga tgattcaaat atttctctag catacatgaa tgaagatgct ttgaaattaa
     9241 atagttttga acttatcaaa ggaaaattgc caacaaaaga gaatgaaatt gtgttagata
     9301 gtggagcttt aaaggcactt ggttataaaa ataaagactt aggtgaaaag ataaaaatat
     9361 cttataatga ctataaaaat gataaaaaaa ttgaaaaaga gttcgttata agtggaatct
     9421 taaaaactag cgaaatatct gaagcaggta aatattatta tgctattata tcagaattat
     9481 atatgagaaa tactagagat atgtcacaag aagattttaa tatttatgta aaatttaatg
     9541 ataaaagtaa tttatcaata gagcaagcaa aagaaaaatt agataaaatt gctaatgata
     9601 taggacttga tactattaat actgctgtaa atgaaaatta tataaatgct ttaaaaccag
     9661 atatggaaac gattatgggt ggaggttttg taggattagt aattgtatta tctagtattt
     9721 tagtaatata caatatattt tatatttcaa tagttacaaa agttcaagaa tttggaaaac
     9781 ttagagcaat tggtgcaact aagaaacaaa taaaaaatat agtgtttaag gaagggttta
     9841 tattagccgg aatagcaata ccaataggaa taattttagg ctatgtactt gcaaatatta
     9901 taataaaatc atttatggat atagatgtaa aatcatcacg attaccagtt atattatcgg
     9961 ttgtggtaat aagctttata tctgttgttt tatcactttt aaaaccaatg aaagtagcat
    10021 caaaagtatc cattgttgac gcagtaagat atacaggaaa taaaataagt aataagagta
    10081 agagaaaagg atataaaaat ataaatctaa agagattatc acatgccaat ttagaaagaa
    10141 ataaaaaacg tacttacatg acattggcat cacttatact tagtggaact atatttataa
    10201 cagtatcaac tgctttagaa agttttgatg ctgagaaaat ggcaagagaa cactttcctt
    10261 atgatataga agttagacta tctggttatg aaatgaacag tgataaaaat cctaaaaata
    10321 atttgaatat tttacaaatg gataatcctc taagtaaaga tttttttaat caaataaaga
    10381 acatggaagg aattaaaaga atagaatctg caagaagtgt aaaaattggt atggaggact
    10441 atgatgttga atttaagtat gaccttttac aaagtataaa tgaaaatgat gtaaaatcat
    10501 taaacaagaa tctaatagat ggaaaaataa atcttgaaag actgcaaact ggagatgaaa
    10561 tagtaattac tcatgttgat acagcaaaag aaatgggagt aaaagcagga gataaaataa
    10621 gattaacttt atatgatgga gataaaaaga taaaaaaaga atttaaagtt caagcaatag
    10681 ctatgggggt tccaagtttt ggtataggga aggattttat tgatagaact ttaaaatacg
    10741 attcaactag tagcttggga atatatacag aagaaggaaa gtatcaagaa atcaaagata
    10801 gtatcaagaa gattgctaaa aataatggat ttttagagac tgattttata gatagtagaa
    10861 ttgagtcaaa taaggctgtt atttcattta taaagataat gggatacacg ctaacaggca
    10921 taataggtgt gattggattt atgaatcttg tcaacacaat gataactagc atagttacta
    10981 gaaaaaaaga acttggtatg cttcaagcta ttggtcttac taataaacaa ttagtgaaaa
    11041 tgcttaattc agaggcaatg tattacactt ctggaatgat gataggaagt attttatttg
    11101 gaggtatatt agggtatatt gctgttatat ttctaaaaaa gactggatta tcttatgcaa
    11161 catattcttt gccaatagta ccaatcttgt tgatgattgt gtgtattctt atagctcaat
    11221 ttataacaac ttatttgata ggacgaagtt ttaataaaga atctcttatt gatagagtaa
    11281 gatacagtga ataaaatgca aatatttgtg gttagtttaa ctagcaaaaa gcatataaaa
    11341 aatatagtta aagagattca taattaaata ggttaaagca tagtacagag caaacaattt
    11401 aaacggatgt ttgctctgta ttttattatg taatcaataa tatatctaat atactgatat
    11461 tattcatgtt agaaaatgta tcggtacaat tctaaaaaag tataaaattg taccatataa
    11521 aatgaaaatt ttgtataata atcgtataga aaataaatag gagctgatat atatgaataa
    11581 gaagattaat tatgcaccag gaccaactga aactagggaa aatgtaagat tagcaagagc
    11641 agaaaaaaca acaaatccag atatagatat tgattttgtt gaattttata aaaagacatg
    11701 cgagaagttt ggcaatatag ttggaacaaa aaacgatgtt tacatattga gtggtgaagg
    11761 aatattaggg cttgaagcag catgtgcatc acttaccgaa aaaggagata gagtacttgt
    11821 aattgacaat ggtatatatg gagaaggttt taaagatttt gtaacaatgt atggtgggga
    11881 gtatgtgctt ttttcaagtg aatatactaa aagtatagat attgatgaat tgaaagcatt
    11941 tttagatgag gatagtaatt ttaaatatgc aacagtagtg cattgtgata ctccaactgg
    12001 cgtattaaat gatgtaagca aaatatgtcc attacttaaa gaatatggaa tattaactgt
    12061 agttgattct gtagcaggta tggtaggaga aagattaagt gtggatgaat ctaaaataga
    12121 tataatatta ggtggttcac aaaaagctat ttctgcacca gcaggactta ctatagttgg
    12181 aataagtcaa gatgcaaaaa attgtataaa aaatagaaaa actgatgtaa taggttttta
    12241 ttgtaattta aatatatggg aaggctatta tgaaaaaaaa tacttccctt atacaatgcc
    12301 aataagtgac ataatggggc ttgataaagc acttgataat atattagaag aaggaataga
    12361 aaatgtatta gatagacatg aaaagatagc ttatagtgtt agaaaatcta ttgaggaata
    12421 tggtctagaa ctatttttag aagaaggata ttctaataca gtcacagcta taaaaattcc
    12481 tgaaagtata ggagctttaa aacttactga ttatatgctt aaaaattata atacacttgt
    12541 tgcaacatca ctaaatcaat atatggatac tattttaaga ataggtcata tgggagagaa
    12601 tgcaaatcta aataaaatag agcatgtact aaatgtcttg gataaaagtt taagtgcatt
    12661 aggattcaaa ggaaatgaaa atttgttgaa tttatttaat aagtattatt tttaatagat
    12721 agggggatat agtatggaag ttacagctaa gcaaaaattt acaacgaaag acatggtgga
    12781 aacctcacta ttaatagcat tagtatttat tgcaactaaa tttataaata taagattacc
    12841 aatatctata aatggtgggc ttgtgcattt aggaacagca atgttgttca tatcagcaat
    12901 agtatttgga agtaaaaaag gtgctttatc aggagctatt ggtatgtcac tttttgattt
    12961 aatatctggg tggactttat gggcaccgtt tacctttata gttagaggag ttatgggtta
    13021 tttacttgga aagattgcat gggcaaatgg taataatggg aataattttt taatcaatgt
    13081 aataggaata tgtgtctcat caatttggat gttatttgga tattatataa cagaagttat
    13141 actttatggt aattttatag tacctcttac ttcaatacca ggtaacttaa tgcaagtatt
    13201 gataggtctt ataatagcgc ttcctatttc taaggtattg aaaaaatgta taaaataaat
    13261 ataaagtgaa ctaaaattta tgatagcata aattatactg tataatatta agatggagta
    13321 cataattaaa aaattttagt atagaggtta gttcacaatg ttatcagtta caagatattc
    13381 aatcaaaaat gttcgtttaa agccgtgggt aaagtttatt tggtattttg aaacaaaatc
    13441 aaatatactg ttaaataata agttacttcc tacagacagt atagatatta ttcttaatct
    13501 ttctgatgtt atggaatata aaattgaaaa tcaagactat actgctagta atatgcattt
    13561 taatggaata agggataaac atggatttat tattcaacat ggaaatatac gtgttatagg
    13621 gatttctttt tatccatttg gtctctatcc atttttaaag ataccaattt cagagtttaa
    13681 cagacaaatt gttgacttag aagcagtatc agaactattt gccaaaaaat tggaagagtc
    13741 attaaatcca acacaatcag tagaaaaaat tgttttatat ttagaagaaa tgttattatc
    13801 catattagaa gaagatttaa tttcaaataa atctgtaaag cttctaaatt catttatata
    13861 tgataataga tatagcaata taaaagcatt ttgtgataat acaaatataa atattaaaac
    13921 attggaaaga atctgtttaa aatatacagg gtatacacct aagattttaa agagaattta
    13981 cagatttaaa atggcaagta gtcaacttat ttataattat aaagataatg aactttttga
    14041 ttttatttat gaaaatgagt actatgacca agcacacttt ataaaagaat ttaaaaaatt
    14101 ttcagggacg tcaccaatta aatttattgg tgagaataag acgattaaag aaaatataaa
    14161 gtacaactat ttataattat actgtcgatt ttttacaata cctccaattt attttttgat
    14221 atgatatagg aaaataaatt ggaggtttta catatgaaaa ttattacaga aatagttgag
    14281 tttaatgtgg aagaaaattt atcaaaagaa gagtttatag aaattgtaaa tgacttagaa
    14341 ttaaaatttc attctattca atctggtttt attgatactg agcttttata tgatgaaaag
    14401 tctcaaaagt ggattatgat acaacattgg gaatctatag aagacctcaa gagtgcttct
    14461 agtaaaatgt ttaaagattc ttcaactgag aaatttagaa aagctttaaa gaatcaaact
    14521 gttaagatga atataatacc acaaattaaa agttggaaat tatagaactg aatgtaaaag
    14581 atagttaaat agtatcaaac taaattagcc aaataacatt ttgtatttaa attgttattt
    14641 ggcttttagt gtttacttta atatgagtgt tatatactag aaaaaccaac aaactgttag
    14701 agtgtggatt ataataatgt catatttggg agctaaaagg cttaatactg atttgtcaat
    14761 atgtcttttt agcatgttga acaggtatat tgccatgctt tcattatttt atttttttgc
    14821 cttatactgg actacatttg cagtttccta atatatttgg ctcatattta aatgccatta
    14881 tctaaaagta ctattgctgt acgagaggca ataggcattt tagaagaagg aggagttgag
    14941 ctaaagtata catcataatc tcttaagttt tctttttttg tgtttcctcc aaactctacc
    15001 atatcagtat ttaatatcgt tttgtagttt gtattttgaa actttgaaat atcaactgat
    15061 tgtattagat tagggtggaa attaaatata aataaatatt tgcccttact gtacaccaaa
    15121 atctttttat cattatccat atgtataagt tcatgactat tttggataaa aatatcactg
    15181 tattttgtaa gttcgagcat agatttatca aaatttaata attgtttata ctttaagttg
    15241 ttatcttctg aaaggctcca ttgtcttctg gcatacttat agctccaatt gttgccttct
    15301 cgtggaaaat caatccactc aggatgtcca aattcatttc ccataaaatt tagatatcct
    15361 tcacctgcta gagaaaaagt tattaattta attaatttaa taagagatat tgctctattg
    15421 ataacatggt tatttgagtt tatttccata ttccagtaca tttccttgtc agctagccaa
    15481 aaaataattg ttttatcgcc aactaatgct tggtcatggg attcacaata tcctatattt
    15541 ttttctccag gtcgtctagt agtaagttca taccacattt ttcccaaatc ccagtcttca
    15601 tcagataagt tagagatagt ttttatccaa aaatcaggta ctcccattgc gagtctatag
    15661 tcaaatccaa ttccgccgtc ttttataggg atacacatac caggcattcc actcatatct
    15721 tctgcaatac ttatcgaatt aggtttaatt tcttttatta attcatttgc aaattggaga
    15781 tatgttatag cttcaatatc tgtattcata ctaaaatatt tttcataact atcaaatgaa
    15841 actccaagac catggttatg atataacatt gaagtcacac catcaaacct aaagccatca
    15901 aaatggtact catttaacca aaattttata tttgaaagca aaaaatgtat tacttcaggt
    15961 ttaccatagt taaaaagttt tgtaccccaa gcaggatgat ttccctttga acctgagtgg
    16021 aaaaattgat gttcacttcc atcaaactca tttattcctt ctaaagtatt ctttacagca
    16081 tgagaatgaa ctaaatccaa taaaacagca atccccattg aatgagcagt gtttatgagg
    16141 ttttttaagt cttcaggagt accaaaccta gatgagattg cataaaagtt tgaaacttgg
    16201 tatccaaaag atgcataata tggatgttcc ataatagcca taagctgaat ggtattgtat
    16261 ccagcttttt ttatcttagg aagtattttt tcagtaaatt cattataagt tccaatagat
    16321 tcgctttcag tagacatacc tatatgacat tcatagatta gtggagaagt tatgtttttt
    16381 aagtcaaaat tattgtcagt ccagataaat ggagtttttg gttgccatat ttgtccatta
    16441 aatccaccaa aatctttttg aattactctt tttatatata atggtatacg gtcaaatgtt
    16501 tttccatttg cagtaacttg tacctttacc tctgatttgt gaggtaagct attttgacca
    16561 ggaataaata tttcccaatt accagaccca atttttctta gaggatgaga ttttctattc
    16621 caattattaa agtcaccaat tagtgacaaa ctatctgcat ttggagccca ttccctatag
    16681 aagaatccat catttgtctg atgaaatcca tagtaaagat gagcatttgc aaaagaacta
    16741 aaatctttat agtttttcaa tgtttttgat ttcatatcct cataatgttt cattcgcttt
    16801 tttatatcag attcaaatgg ttgtaggtat acatcaatat ctaaaatttt aaatcttttt
    16861 tgacttttca ttgtatttct cctccataat aaatattaca tttaaaaaat aatatttatc
    16921 tcatactatc ttctctgtat aattgatata tattatataa tcctaataaa aagcttaagc
    16981 caccaatgat taatttcata atttacctcc ctagtattta tacttatata tcgtattcat
    17041 caaaatttaa aagtttgttt aaaactcttt taataatatc ataatcaaag cctttatagg
    17101 aaagatgttg agaaattttt tgatatattt tctttttatc ttctttcttt actctttcat
    17161 accttttttt tgctagatac atagcatttt caaattcagt gtccttgtct aattcagata
    17221 ctgcttcatt gattgtactt ctattgatac ctttattata taaattctgc ttaattctgt
    17281 ttttgccaca tttatttagg ttaacatttg tgttaacaat tttttgagct aatacactgt
    17341 catttacgag attataactt ttaagaaaat ctatgaccct ctcaattata ttttcatcaa
    17401 attctgatga tagtttttct tttaattttt tttcagattg gtcagcatga gataaaatat
    17461 ttaatgcttt atttttagct tttatataca tttcatcatc taaaataggt tttaattctt
    17521 cttcatttat ttcattccct ttttttaaat taaatgtata aacaagttct ttaaatatag
    17581 caataaaaaa tttatcatct acataaatat taactctatc atcatttctt ttttgttgtt
    17641 ctatttttgt aattataccc ataagtcctc cttttttata tgtaattata ttaacaaaag
    17701 caactaataa aatttaacaa aaaacttata tttgtatgtt atattttatc aaaattaaga
    17761 caaaatagta aaaagttatt tttattaaaa agtaaagcag aatcatgtta aataattatt
    17821 ttttaaagtg ttttttagca tataaatata attatatacc caaaaaaaca aattataagc
    17881 tttaagaaat aaaaaataat ggtacaatag tattataaca ttaaaaggag acaaaatatg
    17941 agaagtgaaa atctagttaa gatggcagag ttgaaaaata ctgaaaaact agagaaattt
    18001 aatcgccata aaggcaaaat caaaataggt atattgggag gtacttttga ccctatacac
    18061 tatgctcatt tagcaacagc agaatttatt agagacaagt atgacattga caaaataatc
    18121 tttataccat caggaaatcc accacataaa ttatgtatca ctacagataa gtatgataga
    18181 tataatatga cacttctagc aactgaaagc aacgaagatt ttttagtttc aaaagtagaa
    18241 atcgaaagaa agaaaaggac ttatacaata gatacattaa aatacttaaa gaaaaaatac
    18301 aaaaatgcag acatttattt tataacaggt gcagatgcaa tttgtagtgt tgaagagtgg
    18361 aaagatgtaa agaaaaactt tgagttagct acttttatag ccgcaactag accaggaatt
    18421 agtttgttaa ggtcacaaga gactatagaa aaactaacta agaagtataa tgctgacatt
    18481 ataacagttt atgttccatc actggatata tcgtccacat atataaggga acagctaaat
    18541 gaaggcaagt caatacgtta cttagtaccc gaaaatgtag aaaattattt atatgaaaat
    18601 aaactatatc aatatggaga tgattaaatg aacttagaaa atattaacca aagactaaat
    18661 caaatgctac cagtggggag gcttagccat tcaagaaatg ttgcaaaatg tgctgaaaaa
    18721 ttatgtgaga tttatggatg tgacaaagaa aaagcttact tagcaggaat gattcatgat
    18781 tgtgcaaagt atttaagtga caaggaaata gaggattatg taaataagta tgaaatatat
    18841 ttagacccaa tagaggatgg aaatcgttcc ttgtctcata gtgtaattgg ggcgtacata
    18901 tgcgagtatg aatttgaagt agaagatgaa gatattataa atgcaatcaa atatcataca
    18961 acagggagag aggacatgtc tcttttagaa aaaataattt acatagctga tttaattgaa
    19021 gaaggaagaa aatttccagt tgtagataca ttaagagaat tagcatatgg agggaagctt
    19081 gatgaagcac ttttaacttc ttttaataat actttaatgt ttgtcatcaa taaaaaagaa
    19141 gagatacatc caagaacagt tatggcaaga aactatctaa ttaaagaaaa gttattataa
    19201 aaaagctata taaaggcaaa catattaata aatttaaatt tgactatgtt acaaaatcgt
    19261 attaatatgt tacaataact aagtgtattt taattaggaa agtgaggaat ttttatatga
    19321 cagtggaaca aatgacaaaa atagcatatg atgccataga agataaactg ggtcaagata
    19381 cagttataat aaatatagga aaagtttcta gtttgtgtga ttattttatt attactacag
    19441 catcttctca gagacaggta aaagctattg ctgacaatgt ggaagatgaa ttagcaaaac
    19501 taggattaga gccaagaggt aaagaaggac aaggaactca aacttgggta ctccttgatt
    19561 atggagacat aatggttcat gtatttaatg aggaaaatag agggttttat aatttagaaa
    19621 aattatggaa agatgcacct tatatagata ttgacacatt agcataaaca tgataatata
    19681 tgttattata attagaaaat ttcaaaaatt atattaaatt gaatagacta gagcagtaaa
    19741 agattatatt ttttcagaga gttagtggaa ggtgagaact aatattatat aactttgaac
    19801 ttactagatt aaaatctatt ctccatggct ggattaaaag ctatctaaga gagtctatat
    19861 atgtagtagg ctaattaggg tggtaccgcg aagatttatc ctcgtcccta aacgtaagtt
    19921 tagtgacgag gattttttat tttcaaagaa ttatttggaa attataagta aataatacta
    19981 aataaggtag ttatacacaa ttaaaaatag gaggcaaaat atgagcgtat ataattttaa
    20041 agaagtagaa tcgaaatggc aaaaaatttg gaaagataat aatcagtata aaatggatac
    20101 tgcacaaact gaaaaaccta attattacac attagaaatg ttcccttatc catctggaaa
    20161 aatacacatg ggacatgtta gaaattattc tataggtgat gttgtcgcaa gattcaaaaa
    20221 aatggaagga tataatgtac ttcatccaat gggatgggat tcttttggat taccagctga
    20281 aaatgcagct ataaaacatg gaatacatcc gcataaatgg acaatggaaa atatagaaga
    20341 aatgaaagag caattaaatt tattaggtat tagttatgat tgggataaag aagttgcaac
    20401 ttcaactcca gaatactata gatttacaca agaaatattt ttgaagtttt tagagcatgg
    20461 gcttgcatat aagaaaaaat catatgttaa ctggtgtcct tcttgtgaga ctgttcttgc
    20521 aaatgagcaa gttgttcaag gagcatgtga gagatgtaaa gcaacagtat taaagaaaga
    20581 tttagaacaa tggtatttta agactactga atttgcagaa gaattactta atgacttaga
    20641 tacattagat ggatggccag aaaaagttaa aacaatgcaa aaaaattgga taggtaaaag
    20701 tacaggagca gatttagttt ttgatataga tggaactgat aaatctatga cagtatttac
    20761 aactagacca gacacaactt atggagttac ttatatggta cttgctccag agcatgaatt
    20821 agttaaagaa ttggttgcag ggactgaata tgaagcagat gttgaagctt ttgttcaaaa
    20881 aatgcatact atgacagaaa tagaaagaac agcagctgat gtagaaaaag aaggaatgtt
    20941 tataggtaga tatgttataa atcctttaaa tggcaagaaa gttcctctat ggatagctaa
    21001 ctatgtatta gttgagtatg gaacgggtgc tataatggca gttccagcac atgatgaaag
    21061 agatagagat tttgctgaaa aatataattt agacataatt gatgtaataa cagaagataa
    21121 caaaatgata aattcagaag aatttaatgg attagatgct tcagaaggat ttgaaggaat
    21181 aatcgataag ttagaaaaag aaggtagagg aaagagaaca attaattata gacttagaga
    21241 ctggttagtt tcaagacaaa gatactgggg ttgtcctata cctgtggttt attgtgatga
    21301 atgtggaata gttccagtta aaaaggaaga tttaccagtt cttttaccaa cagatgttga
    21361 atttactgga aagggagaat ctccacttac gacttcaaag caatttatgt caacaacttg
    21421 tcctcactgt ggaaaaccag ctagaaggga agttgatact atggatacat tcgttgattc
    21481 ttcttggtat ttcttaagat atgtggatag caataatgaa aatgaaccat ttagtaagga
    21541 attagttaat agatggcatc cagtagacca atatataggt ggagtagaac atgctataat
    21601 gcatttgctt tatgcaagat ggtttgtaaa ggcatttaag agtatgggaa tggtagactt
    21661 caatgagcca tttaaaaact tacttactca aggaatggta cttatggatg gttctaaaat
    21721 gagtaagtct aaagggaata cagtatctcc tatggatata atagatgagt atggagcaga
    21781 tactgcaaga cttttcgtat tatttgcagc accaccagaa agagatttag actggtcaga
    21841 gcaaggtgtt gatggatgct ttagattctt aaatagggtt tatagattgg ttgacgagtt
    21901 agcagatgtg gttaaaaagg atgtagagtt tggagaatta aattcccaag ataaggatat
    21961 gagatatact atacactcta cacttaaaaa agttactgct gatttaagtg aaaaattcgg
    22021 atttaatact gctatatcag ctcttatgga attaataaat gatatgtata aatataaaga
    22081 gttagataat ataaacgaag cagttataaa agaaggtgta caaactatag taactataat
    22141 tgcaccattt gcccctcatt taggtgaaga actatggact atgataggta aagaaggaag
    22201 tgtatttgat atagactggc ctaaatatga cgaaaaagct ttagtaaaag atgaaataga
    22261 agttgttgta caagttaatg gaaaagttag aggaaaatta actgttaact caaatatatc
    22321 taaagatgag atggaaaagg tagctttaga agatgaaaaa ataaaaggat tagttgaagg
    22381 taaaactata gttaaagttg tagctgtacc taaaaaatta gtaaatatag ttgttaaata
    22441 actcaaaaat atgaacaaaa atataaattt ataaaataaa tgaggctgtc tcaaaataga
    22501 gataagcctt ttttcattgt tgaaattaca aaaatagatt tgattttata caaaaaaatg
    22561 attaaagaac taaaaagttc attcttatac actctaattg ttgttttaaa tattattata
    22621 attaatgtta aatcgtgtta atgtattaag tggtatgtaa attattcagt ttctaaagtg
    22681 ttgttcatca aggaactgct ctaagcttaa acgaaaaaaa tataattatt gtatttttaa
    22741 aatttttttt atataatgaa aacaggcaaa aaaggtgcgt atctatgctt tatcttaaac
    22801 acccagacaa cgagattcag attcatataa taactaccat gtttctaaaa aggaacttgc
    22861 aaaaaagata ggcgtttcaa cttcccagtt gattaaaatt atcaccagga aaacaaggat
    22921 ggtcagcagt agtattctaa tgggtgttgc tgatatatcc aagttgggat tgtttgagga
    22981 tgttatcaaa tgacttgtga taggtgcagt agatgtggag attcctgata cggatttatt
    23041 ctcaggggat gtttgcttca ggagatatgg agcgtaacca gtttattgat atggtaacca
    23101 tttttttgtc taatttgatg aaagaatccc aagaatacca tacagaagta agacaggaat
    23161 taactggtgg tataaactga taaaggcaaa tttaaaaatt ttgataggat gattttaaga
    23221 tttatcttct attttgtttt ttagaaataa ttgaaatgtt atgagggggt gtgtacaaat
    23281 ggagactgta tcaaataatc aatcaagtgt agaaattcaa ctcagtctgt ttgaagctat
    23341 caaagattta ttgttaatta atcctatttt cttgttggga attatcttga ttggtatttt
    23401 cttagttgta atattcaaga aaaatagtga tattccaaag gtaaaaacat ctattttatc
    23461 actggcaatg tattactact tatgtttaac actatcgcat attgttggta tccctacatt
    23521 cagtgaatat ataagactgt cacagttagg ggaaacgttc tttaatccaa acataaatct
    23581 tattcctttt agtgatggat ttagtttgag ttttattttg aatattttac tttttgttcc
    23641 attaggtttt ttatgtccgc ttatcagcaa aacctttgaa cggttgagaa atacattctt
    23701 gattggtttt ggattatctc tctttatcga aactattcag ctttttacgt tacacagagc
    23761 aaccgatata gacgacttac tcacgaatgt aattggaaca ataataggct atttctgctt
    23821 caaactgatt gctaaattgc gaattgtaaa actacattca gatgatgact ctatggaaca
    23881 ggattacaca gtatgtattc cgattgccat tataatagtt gcccttatat taggattttt
    23941 tagttaagtt ccaaatagct aatttgggaa tccagacaat ataacagata gtatgttacc
    24001 ttttacagga gcatactgtc tgttatgttg tcaacatctc attttatggt ttgtgtgtat
    24061 tttcttgtaa actgaactaa ataattaaat agaagttatt agaattagca atagattcct
    24121 ttttcaaaga aatctttgat ttcatcaagg ttatcagttt ttccaagtgc taagataagt
    24181 tttattctag ctttttgacc tggcaagtcc ccaccaaata tacaaccaag attttttaaa
    24241 tctctaccag aaccaaagta accataacta tcgaatactc tacctgaatg acatctagaa
    24301 actataacta caggaagtcc tttttctcta gcatattcaa ctccagaaag catttgagga
    24361 ggaatattac ctatacccat tgcctcaata actatacctt tataaccact atcaacagca
    24421 aatcttataa agtcagcatt ttctcctaca tatgctttaa ataaggcaac tttagattct
    24481 accttatcag tatctataat tgttctattt acaatatctc ttgtaaggac aagttcgttg
    24541 cagtcaatta ttcctaatgg accactagtt agagatttaa atgtatttag agaaagagta
    24601 tttgttttag tagcttcaga agctaataat acttcattgt ttaaaactac aaggactcct
    24661 tttcccatag catctttgga tactgcagta caaactgatg cagataaatt acttgaacca
    24721 tcatatccaa gttcagaact acttctcata gcacctgtga ctattacagg tttgatgttg
    24781 tcaattgtaa ggtctagaaa ataagctgtt tcctctaaac tatctgtacc atgtgttatt
    24841 acaacacctg taatatcttt tcttgctaat aaatcattta cataattttt caattccatc
    24901 attctataag gtgttatgtg aggtcctgga atttcatcaa aattaaaaac ttcaacatct
    24961 gcaactttat caatgttggt aaccattgat agtatttgtt cacctgataa agttggtata
    25021 gctgctccga ctttatcatc tactgtcata gatatagttc caccagtaaa aactatggca
    25081 acttttttct taatactcat acgattctct gcctttcata aataaaattt ataattccga
    25141 taggattata tcaaaaaaag tagttaaggt ctacataaac tcttttattc atataattta
    25201 gatatttcag tgtaatataa ttatttctta attttttgcc attcaacgat ataaccattt
    25261 aatatctctc ttatggcttt tcctatctta gtataagctt catcatatag atttgctaga
    25321 gatattctaa ttgaccattc agggccatag aatccatttc cactaagtaa aactactgaa
    25381 tagtcattag ctaatttata aagaacatct actggagatg cagttttact tataaattta
    25441 aataagtctt ctccgtagtt taatttagcc cattcttcaa tatcaaattg agtgtaatat
    25501 gcagcattgt ttttgttttc attaattgga agctctaaag cttcaaataa tagtttttga
    25561 cgtctatggc agatactcat attgagtttt ttgtaggcat ctactttgtc aatcaatgca
    25621 aaagcagaga aaaacgccat ttgtacttgc tgtggagtag aaagaccagc agtatggttg
    25681 agagcaacaa gtctgctatc agcaacaact ctttccataa atgaaagact agaagggtta
    25741 agactcatat cactgtaacg cttatctact gattttttta attctccagt taaatcattt
    25801 attttcttat caaaaacatt tttcttatga agggcaaatg taccaagtct ccatcctgta
    25861 actccaaaat attttgaata agaatatact ccaacagtgt tgtaaggtag ttttgacatt
    25921 aaggatgaaa aatcttctac aaaagttcca tatacatcat ctgatattat cattaaatct
    25981 ttattataat tttcaattac atctattaga ttattacaag atatttcatc catagctata
    26041 gaagcaggat tattaggatt aacaacgaat aaagctttaa tagatttgtc acgaagtttc
    26101 tctaattctt tttttgtata ttgccatgta tgagcacctt tttcatcaac ttcattagca
    26161 tttatattta ccactttaaa gtcgtaacga ggaaggttag gtatttcaag ataaggtgta
    26221 aatattggag tcattaaggc tattgtatct ccttttttta gaagttcatt tgccattaat
    26281 gaatcaaata tatagcacat agcagctgta gcaccttcaa cagcaaaaat ttctacatca
    26341 ccatatttac ctgatatatc atatttcatt tcttgaacta ggtaatcgtg tactattttt
    26401 tccatatgag gtaacattct atctggaagt ggatagttat caccaataat tccatcagct
    26461 agttcatgaa caaattcatc tccatcaaat ccaagttcat ttataccaaa gtttataata
    26521 tctctagcta aatatgctcc aggcatatct atattttcat ttataaattt aaaaaatctt
    26581 tctttaatac cctttttttg aggcattcca gccaaatgac ctaaatccca attagaacga
    26641 gtttcagtaa tagcgaaatg accaaaagta aaaaaggctt ctctagcagt tgaacaagtc
    26701 cagtttggat ttccacgacc tgcatcaagt aattctctgt tattttcttt tgcagtgtat
    26761 tttgcaatat ctattaattt gtccttaaat tcaaaagggc ttatttttcc atatatgttt
    26821 ttgatttctt tataagtatg atttttcatg ttaaattcct ccttatgtaa taagtttcac
    26881 atttttataa taaaataaac aagttgcaaa aatgattata ctttaattat aataggttta
    26941 tagaaaaagt aaagtatgaa attaaaataa taactctatt tagccttatt aaatatatgt
    27001 taaaataaaa aatacctttt aataaaaaat gattgttata taaaataaat attcaattta
    27061 attatcacct tagttttttt attgtatttt attatat
//
LOCUS       DAEPOZ010000027        27054 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.027, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000027 DAEPOZ010000000
VERSION     DAEPOZ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27054
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.027"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            56..589
                     /gene="pyrR"
                     /locus_tag="KR440_000931"
     CDS             56..589
                     /gene="pyrR"
                     /locus_tag="KR440_000931"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBH0697992.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            873..2084
                     /locus_tag="KR440_000932"
     CDS             873..2084
                     /locus_tag="KR440_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBH0697993.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKIVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLLLRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISIGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            2383..4035
                     /locus_tag="KR440_000933"
     CDS             2383..4035
                     /locus_tag="KR440_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBH0697994.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEYLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGLLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     IQDGMEIISKNLLEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGCFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYIISRNKKTPSRDKQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQITKLKNSF"
     gene            complement(4250..6025)
                     /locus_tag="KR440_000934"
     CDS             complement(4250..6025)
                     /locus_tag="KR440_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HBH0697995.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDSLHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEIANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIVLSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            6321..7355
                     /gene="ltaE"
                     /locus_tag="KR440_000935"
     CDS             6321..7355
                     /gene="ltaE"
                     /locus_tag="KR440_000935"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBH0697996.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGSKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            7642..8472
                     /locus_tag="KR440_000936"
     CDS             7642..8472
                     /locus_tag="KR440_000936"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBH0697997.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKINLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGVLEV
                     "
     gene            8476..9357
                     /locus_tag="KR440_000937"
     CDS             8476..9357
                     /locus_tag="KR440_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBH0697998.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            9387..10004
                     /gene="gmk"
                     /locus_tag="KR440_000938"
     CDS             9387..10004
                     /gene="gmk"
                     /locus_tag="KR440_000938"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBH0697999.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLEELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            10007..10273
                     /locus_tag="KR440_000939"
     CDS             10007..10273
                     /locus_tag="KR440_000939"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBH0698000.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            10278..11477
                     /gene="coaBC"
                     /locus_tag="KR440_000940"
     CDS             10278..11477
                     /gene="coaBC"
                     /locus_tag="KR440_000940"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBH0698001.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLAFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            11716..14241
                     /gene="priA"
                     /locus_tag="KR440_000941"
     CDS             11716..14241
                     /gene="priA"
                     /locus_tag="KR440_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBH0698002.1"
                     /translation="MNNILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVI
                     KIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNEIELKEKISTLSDKNREIVE
                     KVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSL
                     KLQKEIRYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            14259..14699
                     /gene="def"
                     /locus_tag="KR440_000942"
     CDS             14259..14699
                     /gene="def"
                     /locus_tag="KR440_000942"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBH0698003.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            14714..15643
                     /locus_tag="KR440_000943"
     CDS             14714..15643
                     /locus_tag="KR440_000943"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBH0698004.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTIYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            15653..16405
                     /locus_tag="KR440_000944"
     CDS             15653..16405
                     /locus_tag="KR440_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HBH0698005.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNREDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNVGELI
                     KLKEKTNVKIFVATGGTLARKIIMDNKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDMMDIEKAIDFLTGKNILICN"
     gene            16474..17175
                     /locus_tag="KR440_000945"
     CDS             16474..17175
                     /locus_tag="KR440_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBH0698006.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            17237..18562
                     /gene="rsmB"
                     /locus_tag="KR440_000946"
     CDS             17237..18562
                     /gene="rsmB"
                     /locus_tag="KR440_000946"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBH0698007.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            18570..19601
                     /gene="rlmN"
                     /locus_tag="KR440_000947"
     CDS             18570..19601
                     /gene="rlmN"
                     /locus_tag="KR440_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBH0698008.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGNIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            19621..20373
                     /locus_tag="KR440_000948"
     CDS             19621..20373
                     /locus_tag="KR440_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBH0698009.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            20390..22405
                     /gene="pknB"
                     /locus_tag="KR440_000949"
     CDS             20390..22405
                     /gene="pknB"
                     /locus_tag="KR440_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBH0698010.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     EEEEEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGSSNSGNSGGNSGNNGGNSGGSNSGNSGGSSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            22513..23250
                     /locus_tag="KR440_000950"
     CDS             22513..23250
                     /locus_tag="KR440_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBH0698011.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKADISNREDVENMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            23348..24232
                     /gene="rsgA"
                     /locus_tag="KR440_000951"
     CDS             23348..24232
                     /gene="rsgA"
                     /locus_tag="KR440_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBH0698012.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDEDEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            24237..24908
                     /locus_tag="KR440_000952"
     CDS             24237..24908
                     /locus_tag="KR440_000952"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBH0698013.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            24917..25558
                     /locus_tag="KR440_000953"
     CDS             24917..25558
                     /locus_tag="KR440_000953"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBH0698014.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTVSVTAI
                     KGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            25689..26318
                     /locus_tag="KR440_000954"
     CDS             25689..26318
                     /locus_tag="KR440_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBH0698015.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSKHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            26373..27029
                     /locus_tag="KR440_000955"
     CDS             26373..27029
                     /locus_tag="KR440_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH0698016.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGASIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
BASE COUNT        10759 a         2752 c         5066 g         8477 t
ORIGIN      
        1 agaaaggagt agatttgtca aatagatata ttatatcttt gacaataatt atgatatggt
       61 agaaaaagct caattaatgg atgaaaaggc aatagcaaga gcaattacta gaataagtca
      121 tgaaataatt gaaagaaata aaggtgtaga aaatctagtt ttagttggaa taaaaactag
      181 aggtgtgcca attgcaaata gaatttctaa gaaaatagaa caaatagaag gaacaaaagt
      241 agatacagga gatattgata taactttata tagagatgat ttggaaaaaa ttcatgttga
      301 acctgtagtt aaaggaactt atttagattt taatgtaaat gataagacag tagttttggt
      361 tgatgatgtt ttatacactg gaagaactgt gagagcttca ctagatgcga ttatagatat
      421 aggaagacct aaatcaatac aattagcagt tctagtggac agagggcata gagagttgcc
      481 tataagagca gattatgtag gaaaaaatgt acctacttct agacatgaaa ttatatctgt
      541 cagtttatta gaaattgatg gagaagattc agttacaatc aaagaataat tagttttaag
      601 tattatataa aagcttagca ttgaatagtc agggaggagg ataaagctta catagattgc
      661 acaaaataaa tattataatt ttatggatgt gagtcttaat gtagttttta ttctccttta
      721 tttgagtttt tgatgtcgta aaatgtaaaa taatgataaa ttatataata agtatataga
      781 ggttaaaaaa tagaatctag ggaatgataa ttaattaaaa gatattaatt aaacagagga
      841 tttatttata tataataatt ttggaggttt ttatgaaaaa aacaataatg tctttacaac
      901 atctattagc catgtttgga gcgacagtat tagttcccat tttgacaggt tttaatccgt
      961 cagttgcaat tttctgtgct ggtgttggta cattgatatt tcatttttgt acggaaggaa
     1021 aagtacctgc ctttttaggt tcaagttttg cgtttatacc agttatacta gcagctaaag
     1081 aggcgtatgg tggagattta gcatatgctc aaggtggtat aatagtagca ggattaattt
     1141 atattataat gtcgattata gtaaaaatag ttggtgtaaa taaaattaaa ttatattttc
     1201 ctgcacaagt tacaggagct atgatagtag ttataggatt aaatttattg ccaacagctt
     1261 ttagtatggc ttcagctaat tttgtaatag catttataac tttagcaata gcaattttaa
     1321 ccaataagtt tggtagagga tttataaaac aattaggaat attaatagcc gttttttctg
     1381 ggtatataat ctgtttactg ttaaggttag tagatataac tactattaca gaagcaagtt
     1441 tatttgcaat accgaacttt acagttccta agttcagttt aggagcaatt gtgattatat
     1501 ctccagttgt attagctgta tttatggaac atataggaga tatgactact aatggtgctg
     1561 tagtaggtaa aaactttata gaaaatccag gattaaatag aacattactt ggggatggat
     1621 ttgcgactat agttgcaggg tgtttaggag gaccagccaa tacaacttat ggagaaaata
     1681 cagcagtact tgctataact aaaaactatg acccttcaat acttagacgt acagcaatat
     1741 ttgctatatt gcttgcatgt gtaggaaagt ttggtggatt tcttcaaagt ataccaggtt
     1801 cagttatggg tggaattagt ataatgctat tttcaatgat aacttatgta ggattaaaaa
     1861 ctataaggga cagttcttgt gtagaaagta agataaatat attaataata gcagttatat
     1921 tattaatagg tcttggaaca acatatttat ctaataaagg catttctatt ggaatcccta
     1981 taacaagtac agtaaagata actggtctta gtttagctgc aattgttgga attgtattaa
     2041 acagaatttt aaataatcag gattttaaag ttgaagaaga ataaatttaa agaataaatt
     2101 gtatattgaa taactaaaat tagttaattt tctatagaca tttgtaatta atgttgatat
     2161 aatgattaaa aataaatgaa agaaagggca aggtaactag gacaatgaga atataatcta
     2221 ttttttataa tttaaagtat tgtaattttt gaaaagccta ttttgatggc tttatttgca
     2281 atgcttaaat taaggagagt agattatagt ctatacgttt atttgttatg cctatttata
     2341 gaatctctcc ttatatttaa gtgtgttaag gagggatttt ttatgttgtt agtaaaagta
     2401 gagaatttaa agaaatatta tgcagataaa ttaattttgg atatagataa acttgaaata
     2461 ttagaaaatg acaaaatagg tctagttggt tcaaatggtc aaggaaaaac aacattatta
     2521 aaagcaatat tgggtgaaat agaaattgat gaaggatata cttaccttac agaaagctat
     2581 tcttatataa gtcagagtga aaataatatt gaaacatgta gtcatagtaa ggagaagagt
     2641 cttttaaatg ctccagataa atttgaagaa tacttatcag gaggggaaaa ggttaagtta
     2701 aaaatagcag atgcgctgag taataagaaa aatatcataa tagctgatga gccaacttca
     2761 aacttagata aaaaaagcat tgggcttttg gaagatatgt ttaaaaggca tgagggagca
     2821 ttattattaa tatctcatga tagacgtttt ctggatgagt tatgtacaac tatattagaa
     2881 ctagaagatg gaaaactaaa agcttacaaa ggcaattaca ctgattattt aatgcaaaaa
     2941 gatgaagaag taaaaagagc tgattttgag tatcaagaat atgttaaaga gaaaaaaagg
     3001 cttgaaaaag ctcttttata taaaaaagct ttaagtgatg gcataagaaa gacaccaaaa
     3061 agaatgggta attcagaagc taggttgcac aaaatgggtg gtcagactaa taagaaaaag
     3121 ttggactcaa atgtgaaggc tataaaaagc agaattgata aacttgaggt aaagaataaa
     3181 cctaaagttt ctaaagaaat gaacattaag attcaagatg gtatggagat aattagcaaa
     3241 aatctattag aggtaaaaga tatgactcta aagttagaaa ataagctctt gttagataat
     3301 gtttccttta agataaaaag aggtaaaaag atagcattat taggtgataa tgggtgtgga
     3361 aaaagtactc tgataaaaga aatattggct gataaaaatg acaatataaa aataaataac
     3421 aaggtaaaag taggttgttt tgaccaaaat caaagtttat tagatgagga aaagagtgtt
     3481 ctatacaata ctaaagttaa tagttcattt gatgaatctt ttataaggat aaacttaagc
     3541 ctatttgggt ttaaaggtga tgatgtctac aaaaaagtaa aagtacttag tggtggagaa
     3601 aaggtaaaaa tagcactatg taaaataata ttggaagata ataatttttt agtatttgat
     3661 gaaccaacaa attacttaga tataaaatct atggaagcac tggaaaaggc actgataaat
     3721 actgataaaa ctatgcttat agtgtctcat gatagagtat ttgtatcaca tatttgtaac
     3781 tatattatag agataaaaga tgctaaaata agggagtttg attgtaatta tgacgaatat
     3841 ataattagca gaaataaaaa aactcctagt agagacaagc aaatcaaaaa agagaatctt
     3901 ctagtattag aaaatagact tacaactgtt atatctatgt tatctataga aaaagataat
     3961 ctaaaaaaag aattgtatga atcagaatat aatgaattac taaaacaaat cactaaatta
     4021 aaaaatagct tttagtgtta actttttaat tacatctata taagaagact gaaaatattt
     4081 gttgggaata taagtatagt tattaaaaca gaaaagtatt ttgttagaaa attaaaagta
     4141 agctaattag aaagtggcct tattattgta ctttataaga aggccactat ttttatttag
     4201 aatgataatg tgtgcttgtc taacatttat tttgtattta gtgcagaatt tattcagatt
     4261 taaccttaag cttggctact gtttcttctt caggagtaac ataaatagta ctgttagttt
     4321 cataaataac catacctggt ttagaaccac taggtttttt cacatttttt ctcttagtat
     4381 agtctacagg cacctgagaa gaaagttttg acttactaaa gaatgctgct agcatagctc
     4441 cttcaaatac agtattatca gggacttcct taccagcaca ctttattatt acatgagaac
     4501 caggaatatt ctttgtatgc atccataagt catcattatc agcaagtctt agtgtaagat
     4561 agtcattttg cttattgttt ttaccaacca atattttaaa tccatcagaa gacacaaatt
     4621 catgaggttt tgtagatggg atagtatcct ttttactgtt tttcttttga gtcttgatat
     4681 atccaacctt agcaagttct tcttttatat cttgtaattc agctaaattt tcgcagtttt
     4741 caatacttaa aactatgttt tctaagtaat ctatttcttc cttatttaag gaaatttgat
     4801 gagatatctc gactttagca tgtttcattt tgttatactt tttaaagtat ttttgagcat
     4861 tttcagatgg tgtcaagttt ttatttaatt ctattgttac atcattacaa ttttcatcat
     4921 agaagttagc aatttcaata ctttccatac ctttttctac catataaata tatgaagtaa
     4981 tcaattctcc ttttatctta taaatgtctg cattttcgga ttctgataat tcttcttctt
     5041 gttttttaag tttattataa agtctatcaa gcttaacaga tatactcttc tttaaatcag
     5101 atgaacgttg attaattctg tctttaatat ctttagttct atagaaatct tctagaatac
     5161 gagacatact atccttattt atatatgata aatcgctaaa taatgtaagg tttatacaac
     5221 tgaaatcaac taccttatcc acctttttat ctataataat gcaaggggag tatttgttag
     5281 aatttatatc attgaataaa ttacaaaata ctttgtgtaa agaatcaaat tgttcatcag
     5341 atatatcctt tatgatagca ttttgattta cacctgctct gtagcaaatt tctttagcta
     5401 taatagggct tatacctaga aatttagaat atatagactt aaagataggt ccttccaatt
     5461 cttctaggtt tttaataaac aaatccttag atatatcatc aagagggttg agtttgtttt
     5521 gttctggtgg taatgaataa tcatgaccag gtaaaacttg acgaactcta cttatgctaa
     5581 aagggactct ttttatagaa tctataattt tattttcttc accatgagtt attattatgt
     5641 tgctatgtct tcccataatt tcaatcataa tatttttaac tgtcttttct tttaattcat
     5701 ctaaagattc aacacttatt tttattattc tttcaaaacc tatttgagaa atttctacta
     5761 tatttccacc ttgtatgtat tttctaagta acatacaaaa cataggcgct tttagagggt
     5821 tttctttttg ataattactt gtaagataaa ctctaggatt tgaggcactt gcacttaaaa
     5881 ccaatctaaa attttcttta ttatttctta tattaaaaat tacctcatca tcttcaggtt
     5941 gatgtatctt atctattttt ccacctgtaa gttttgatga taattcatcg actattgaat
     6001 gtataactaa tccatctaat gccatttata gcaccccttt ctttgaccaa aatgagtcag
     6061 taaattaatt atacacataa tatacgcttt gtgactaggg ggaatttaaa cgatagagat
     6121 taatatttaa attgtataat tatttatact tattagagag gaaactttat ttttaaaatt
     6181 aataatttaa agtagattaa atataaagta aatatagtat aattattgtg tagagtataa
     6241 gctatgaaag tatatgaact ttacagaaaa aatcaatttt aaggttctat agtatataaa
     6301 tatgaaagaa ggttgaaaaa atgagattta tcgatttaag aagtgatact gttactatgc
     6361 ctactgacga aatgagagaa gctatggcaa aggctcctgt tggagattca atctttagag
     6421 atgaccctac tgtaaataaa cttgaagaat tagcagcagc taaagttgga aaagaagatg
     6481 ctattttcct tccaagtgga acttttggga atcaattagc tctatttact cattgcctaa
     6541 gagggcaaga aataattatt ggtaaaggtt atcacattgt aactcatgag gttggggcac
     6601 ctgctgtaat agctggagtt cagcttagaa ctatagatga agacgaaagt ggagctctaa
     6661 atcctgaact tgttgaaaag gcaattagaa gagatgacat acatgaacca cagacagggc
     6721 ttatatgtgt agaaaatgca tattcaggtg gaactgttgt gagtcttgat aatttaagag
     6781 aaattaaaaa aatagctgag aaacacaact taccagtaca tttagatgga gcaagattat
     6841 ttaacgcagc attaactttg ggtgtagaag ctaaagaaat agcaaagtgt tgtgatagtg
     6901 ttatgttctg tgtgtcaaaa ggtttagctg caccaatggg ttctatactt gctggaagta
     6961 aagaatttat agcaaaagct agaagaaagc aaaaagttat gggtggggga atgagacaag
     7021 taggtatagt tgcagcagct ggtataattg ccattgaaaa aatgacttta agattgaatg
     7081 aagaccatga aaatgcaaaa tatttagctt gtgaattaaa taaaattgat ggcattgaag
     7141 taaacaatgt taatccagat ataagtatgg tattttttaa gatgagtgaa gatattataa
     7201 aagaggatgt tttaataaaa gaattttttg aaagaaatat aaaaattaat aaaatggaaa
     7261 atggtgaata tagatttgta actcatgtag atattactaa ggaagattta gactatgttt
     7321 taaaaacact taaagaatta ataggtgtat gttaatagat atctactaaa aggattatag
     7381 ctggttacca gatgtttaat taaaaaattc atagacagtt taaataaaac atacataaaa
     7441 ttcatataga aatattaata tataaacata gaaagaacta gcttaaaaac ataatataat
     7501 taccctaatt gtaatgacaa ctctccctca catcctcaaa ttatagttgt cattacaaaa
     7561 cttttcccat aaattataag tttatgggaa tttttttatg ttataatagt ttaagcgtta
     7621 atttacgaag gagcgatact tatgaaattt tggaaactac atggaatagg aaatgatttt
     7681 atagcaatag atggaagatt cgaccagata aatccatctg attatactgc tttagcaaaa
     7741 aaagtatgcc atagaagatt ttctgtagga gcggatggat tgttagttgt gaaagattca
     7801 aaagttgcag atgtagaaat ggtatattat aattctgatg gttcaagagc agctatgtgt
     7861 ggaaatggcc ttagatgttt ttgtaaattt gtttatgata attgtatagt taaaaaagaa
     7921 agttttacag tagatacatt agatggtata aaagatataa aaataaatct taccaataga
     7981 gaaataaact ctataagagt caatatggga aaaggaagtt ttatagctaa ggatgttcct
     8041 gtacttacaa ataaagaacg ttttgtgcaa gaaagtataa aggttttaga tagggagctt
     8101 aaagttactt ctatgttgat gggggttcct catacagtag ttatagtaga tgaattagat
     8161 gtagatgatg tttgtaaata tggaaaagag attgaaaata ataaaatatt tcctgaaaaa
     8221 acgaatgtaa attttgtaaa ggtagaagat aagtataata tacatgttta tacttgggaa
     8281 agaggttgtg gatatacttt aggttgtggt actggtatga ctgcatcagc aatagtgtgc
     8341 aatttactta atatggtaga aagtagtgta aatgtaacgt ctcaaggtgg aacagttaaa
     8401 atagatgtag gagatttctc ttatatgaca ggtccagcag taaaaatatg tgaaggtgtt
     8461 ttggaggttt aattaatggc tataagtatg actggttttg gaagaggaga atacaaggat
     8521 gataattatt actttttagt agagtgtaaa acgataaatc ataagtattc agatataaac
     8581 atacgtcttc caagaaaaat ttcattttta gaggataaag ttagaaattt agtaaaaaat
     8641 tatgtaaaaa gaggaagagt agatttatac ataaagtttg acttattagg caaagaagat
     8701 gtaaacttaa attttgatga gggattagca tctcagtata tagacatact aaaagaaata
     8761 aaaaataaat ttgatattat agatgatatt agcgttatga atgttgcaaa gtttcctgat
     8821 attgtgaaaa tagaagaaaa ggaagaagat gaagatttat tgtggtcaat gttgaatcaa
     8881 gcagttgaag atgcattaat caaattaaga gaaatgagaa gtgaagaagg taaaaaatta
     8941 gctgaagata ttgccatgag atgtgattta ttaaaaaatc atatagaaga gattgaaaaa
     9001 tattcaagta gtgttgtaga agattataga gaaaaattga atttgagaat aagtgaatta
     9061 ctggatgacc caagtataat agatgagaat agattagcac aagaagttgc aatatatgca
     9121 gataaaagca gtataacaga agaaatagtt agatttaaaa gtcatatagg gcaacttaaa
     9181 aatactatat ttaaagacga ctctataggt agaaaaatag atttcttaat acaagagatg
     9241 aatagagaaa ctaacactat aggttcgaag tcatctgata taaatataac taatttagta
     9301 gtagaagtaa aaagcgaatt ggaaaaaata agagaacaaa tacaaaatat agagtaagaa
     9361 tacaatatta gaaggggaat gcttaaatgt ttgtgaaaaa aggattattg ctagttgtat
     9421 caggaccatc aggtgcagga aaaggtacta tttgcaaaga actacttaaa gaaaatgata
     9481 caataaaact ttctgtatca gctactacaa gaaaacccag aactggagaa gttgatggag
     9541 taaattattt cttcataagt aaggaaaagt ttgaagagat gatagaaaaa ggtgagtttt
     9601 tggagtatgc tcaaatttat gataattttt atggaactcc aaaagcggca ataatggaat
     9661 gtttagaaaa aggacaagat gtattactgg agattgaaat gcaaggagca aaacaaataa
     9721 aagaagtttg cccagaggga gtatttatat ttgtactacc accatcactt gaagaattaa
     9781 aaaatagaat tgttggaaga ggtacagaaa cagaagctga aatagagaag agatttagct
     9841 gtgcatatga agaaattaaa atgattaagg attatgatta ttttatattt aatgaagatg
     9901 taaaaacatc tgctaaagaa atagaaggta taatatcatc tgaaaagaat aaagttagca
     9961 gatataaaaa tattataata gagaaattta aggaggaatt ataattatgt taaaaccatc
    10021 tatcaatgag gtactagaaa aaatagacaa cagatactat ttagtaggga ctgtttcaaa
    10081 gagagcaaga aaattaattg atggagaaga accatatgtt tcaaataaaa ctaaggaaaa
    10141 gcctgtatgt gtagcaacta aagaagttgc aagtggaaaa ataacatata gattattgac
    10201 agaagaggaa atagagatag aagaagctag acatcatgct gagcaacacc aacaaatttc
    10261 agaagaggag taaggtcatg ctaaaagata aaactgttgt aattggcata agtggcggta
    10321 tagctgcata taaggcttgt gatgttgtaa gtaagttaaa aaaactaaat gcaaatatcc
    10381 atgttataat gacaaaatca gcaacagaat ttgttaaacc acttgcattt caatcgctga
    10441 gtcaaaatta tgtagtagaa gatatgtttg aagaaccaaa aacttgggaa gttgaacata
    10501 tatcattagc aaaaaaagca gatttattct taatagttcc agcaagtgca aatgttatag
    10561 gcaagattgc aaatggtata gctgacgata tgttgacaac tactgttatg gcaaccaaag
    10621 cacaggttct tatagcacca gctatgaata ctaatatgta tgaaaatcca attgttcaaa
    10681 aaaatataca aactttaaag agctttggat ataaatttat tgaaccagaa agtgggaggc
    10741 ttgcatgtgg agatattgga acaggcaaat tagctaatcc agacataata gtagaggaag
    10801 ttttgactaa tcttactaaa gagcaagatt taaaaggtca aagtatgata gttacagctg
    10861 gtccaactgt agagagtata gacccagtaa gatatttaac taataggtct tctggtaaaa
    10921 tgggatattc tatagcaaga gaagctatag ctagaggtgc tgatgtaaca ttaatatcag
    10981 gtcctacaaa tctttctatt ccacaaaatc ttaaaaaatt tgttcaaata gagtcagcac
    11041 aagatttgta tgaagcagta gtatcaaatt taagtgaaaa taaaatagta attcaaagtg
    11101 cagcagttgc agattataaa ccaaaaactt attctgacaa aaaaattaaa aagaagaatg
    11161 atgatttatc aatagaacta agtagaaatt atgatattgc ttatgaaata ggaaaaataa
    11221 aagaagataa gatactaatt ggatttgcag ctgaaactaa tgaccttata gaacatgcca
    11281 aaggaaaaat aaaaaagaaa aatttagatt ttattgtggc aaatgattta actaaagaag
    11341 gtgctggttt tagagctgat actaatattg taaaaataat agatgtagag ggaaatatta
    11401 ctgagtatcc taagatgaaa aaagaagaag tagccaatgt tattttagat aaagttaaag
    11461 cgttgcttga gaaataagac cttttttaaa tatgatattt gtgtatttta tcacagctaa
    11521 agtggctgaa agctgcaatt taattttagc gagtaaaaac tcgcaaatgt cagttaaatt
    11581 gtagttttca gcatttattt tgtcataaag tataaaatat aattaatagt taattattaa
    11641 aataaagtta atattaataa cttgaaattg cttataaatt ctgagatttt aatatcttgt
    11701 aaagtatatt aaaatatgaa caatatatta aatttaagta gaggttttat aatgaaaaaa
    11761 tatgcaaagg taatagttag aagtaatact atatatacag ataatttatt tacatatcaa
    11821 attcctgtat ttttatctga tgtaataaaa atcgggcaca gaatattagt accatttgga
    11881 aaaggtaata aaccaacaga agcctttgta tttcaattta cagatagctt agatgaaaaa
    11941 ataaaaataa aagaaattat tgatatatta gatgaaaatc caatatttag aaaagaagat
    12001 ttagagttgg tttattggat gaaaaataga tatttgtgta cttatataga atgtataaat
    12061 ttgatatatc caaagggata caaattaaat aattataaag tagtcctatt aggtgaaagt
    12121 ttaagtggat taaatgagat tgaattgaag gaaaaaatat caactttaag tgataaaaat
    12181 agagaaatag tagagaaagt gattgatagt aaaggtaaaa ttaaggttga taagttaaaa
    12241 tatataccaa acttaaatag ttctttgtac acaatgaata aaaatggaat tataaaatta
    12301 tgttgggaat ataaaaatca caaaaatgaa aagaaagtat gttatatatc tttgagttta
    12361 gaaagtgata agattgacga ttatatagag caaaataaaa taaatgtggg aagtaaacaa
    12421 aaagagatat taagttttct taaaaataat gaaaatgtag agataaatga tttattggat
    12481 ttattaaatg cttcaaaaca aagtataaat tctctaagta agaaaaagct aataacccta
    12541 gaatttaagg attattatag agagccaaaa agtatttaca aatccgtact aaaaagtata
    12601 aaattaaaca atgaacaaca agaagcagtt gatgagataa agtcgaatat gtttgtagat
    12661 gataaaaaac catacttaat tcatggagta actggaagtg gaaaaactga agtttacatg
    12721 gaaataatag aatttgctct aaatcaaggc ttagacagta tattcttagt acctgagata
    12781 gcattaactc ctcaaacaat agataggcta aagagtaggt ttggagattt agttggagtg
    12841 ttccacagta aactatctga aggagaaaaa catgatgttt acaaagctgt caaagcaggt
    12901 aaagtaagag tgttaattgg agctaggtca gctttatttg caccttttaa ttccttaggc
    12961 ttaataataa ttgatgaatg tcatgagtca tcatataagt ctgagaagaa tccaaaattt
    13021 aatgctatag aagttgctag atttatggca ttaaaaaata atataactct tattttaggt
    13081 tcagcaacgc cctcaattga agagtattat agggcaaaat ctggagaata caaacttata
    13141 aatataaaaa gtagagcaaa tgataagcct cttccaaaca ttgaagtggt agacatgaaa
    13201 gatgaattag ataaaggaaa tagaagtata tttagtttga aattacaaaa agaaatacgt
    13261 tatgcgattg aagaaaataa tcaagtgata ctatttttaa acagaagagg ttatgcaaat
    13321 tttgtatctt gtagaaagtg tggatatgtg tttcaatgtg aaaattgtga tatatcttta
    13381 acttatcata agaaaagcaa tactggaaga tgccactatt gtggatatga gaaagaaatt
    13441 ccaaaagagt gtcctgaatg taaaagtact tatgttaagc catttggagt tggaactcaa
    13501 aaaattgaag aggaattaaa atatattttt ccagatataa aaactttgag aatggataaa
    13561 gatacaactt caaaaaaagg tgctttagat gagatactaa ataaatttaa agataaagaa
    13621 gcagatgtac ttattggaac acagatgtta agtaaagggc tggattttga gaatgtaact
    13681 ttagtaggta ttttgtctgc agacatgata ttaaattttc cagactttaa aagttttgaa
    13741 actacatttc aattgataac tcaagtctct ggaagagcag ggagagctga taaagaggga
    13801 aaagtagtac ttcagactta tgacactgaa cactatgcta ttaagcatgc tattgaatat
    13861 gattatgagg gtttttatga agatgagata aaaataagaa aagcatttgg atattctcct
    13921 tttaataata tgttaagtgt agttgttagt ggtgaagatg aaagattagt gataaaaaat
    13981 ataaaaaata tgcatgcatc attgatttat ttattagaaa aaaggggtat aaatgattta
    14041 ggatttatat taggtccaaa tccatgttca atatcaaaaa taaatcaaaa ttatagatgg
    14101 caaatactct ttaaagatga aaatattgaa attaatctct taaagggtat aataaaatat
    14161 atatgtataa caaaacgaga tttgatattt gataagaata tcaatgtatc tatagatatt
    14221 aatccaaaca gtgtattata aatttaggag gaattataat ggctttaaga caaatagttc
    14281 aaataggaga accagtatta agaaaaaaat caaaaaaggt tgaaaaaatt gatgaaaaga
    14341 taatacaatt attagatgat atggcagaaa ctatgtatga tgcagatggt gttggactgg
    14401 ctgcacctca agttggaata ttaaaaagag tagtagtaat agatattgga gaagagctta
    14461 tagaacttat aaatcctgaa ataatagaaa catctgggga gcaaatagat gaggaaggat
    14521 gtttaagtgt tgttggagaa gctggaaatg ttagaagacc aaattatgta aaggtcagag
    14581 ctttaaatag aaatggcgaa acaatagagt tagagggaga agaactttta gctagagcat
    14641 tttgtcatga gatagaccac ttagatggaa ttttatttgt tgacaaaata gaaaagtagt
    14701 aggagttgat ttgatgaaaa tagtatttat gggaactcct gatatagcag ttccatgttt
    14761 acaaaaaata atagatgaga agtatgaaat attgggtgtt gtaactcaac cagataaacc
    14821 aaaaggaaga ggtaaaaaac taggtatgag tccagtaaaa gaactggcaa ttgaaaataa
    14881 tatacctgta tatcaaccag taaaggctag agataaagaa tttatagata aaattaaatc
    14941 tttaaatcca gatgtaatag tagttgtagc ttttggacag atacttccaa aagaaatatt
    15001 agagattcct aaacttggat gtataaatgt tcatgtttct ttacttccaa aatatagagg
    15061 tgcggcacct ataaattggg taataataaa tggtgaagaa aagactggtg ttacaactat
    15121 gtatatggat gaagggttag atactggaga tatgatacta aagacagaag taaatcttga
    15181 tgaaaatata acagcaggag aacttcatga taaaatgatg aatataggag cagaaacttt
    15241 aaaagaaaca ttaagattaa ttgaagaagg aaatgctcca agagaagtac aaaatcatga
    15301 agaattttcc tatgcgccaa taatgaataa atcattaggt aacatagatt tttcaaaaag
    15361 tgccagagaa atacacaatt tagttagagg agtaaatcca tggccaagtg cttatactat
    15421 ctataatgat gtcataatga aagtttggaa aactaaagtg ttagatgaaa aaagcacaaa
    15481 agatgtaggt acaataattg atgtaagcaa agatgggata aaagtaagta ctatagataa
    15541 tgtactttta attgaagaaa ttcagatgcc gaataaaaag agaatgttgg ttggtgaata
    15601 tataaaaggg aatactatag aaactggttt agtattgggt taggtgaaaa gtatgacaga
    15661 tataaagaag tatttaacaa gtgttgggat tttgatttta atattaggaa ttttgatagg
    15721 agttgttaat ttttttataa taagtttaac tcaaatattc tccttgatga taaatattat
    15781 aataatatca ctaatactta taatactatg ttcaacagca gttacttata gagtaattaa
    15841 aggaaagtat gttaactcaa atataatgaa aataaatttt aatattgtca gtggattatt
    15901 tcctattata tctttcattg catcatcatt tggtatgtca aaaagtgata ttagaagaat
    15961 atatataaag ttaaataatg aatatatata cagtaataaa tataatttta atcgagagga
    16021 tataataatt ttaatacctc attgtattca agagaatagt tgtaaattaa aagttactaa
    16081 tgatatagat aattgtaaag aatgtggaag atgcaacgta ggagaattaa ttaagctaaa
    16141 ggaaaaaact aatgtaaaaa tatttgtagc tacaggagga accctagcta gaaaaattat
    16201 aatggataat aaacctaaag ctgttgtagc tgttgcatgt gaaagagatt tgacatctgg
    16261 aatacaagat attaagaaaa ttcctgtttt aggtgtattt aacaaaagac ctaatggacc
    16321 ttgtgtaaat actaatgtag acatgatgga tattgaaaaa gctatagact ttttaacagg
    16381 aaaaaatata ttaatttgta attaaactgt ttttaagtta agtgaatatt aagaggatgt
    16441 ataataaaaa tatattttag gagagtgtta tttatgtacc ctgtatatgg cggtttttgg
    16501 ggatttgacc caacaatggt tgtattaatt ccagctatat tacttactat atatgcacaa
    16561 tttaaagtga gttcaacaac aaataagtat ttaagagtaa atacacgtcg aggatatact
    16621 ggagagcaaa cagcaagaag agtacttgat tcaaatggtt tgtatgatgt aaagatagaa
    16681 atggttagag ggcatctaag tgaccattat gacccaagaa gaaaggctgt aaggttgtca
    16741 gaagatgtat attatggaac atctataact tctgttgcag tagctgcaca tgagtgtggt
    16801 catgccatac aacatgctaa aggttatgcg cctcttcaaa taagaagtag tttagtacca
    16861 gtagtgaatt ttgcttcaag tatatcttgg tttttaatat ttttaggatt tattatggct
    16921 ggtccatttt taaaaattgg tatactattg ttttcggctt cagtgttatt ccaaataata
    16981 acattgcctg tagaatttaa tgcttcaagt agagcaatcg ttcaacttgg gaatttaggt
    17041 attatagatg aaagtgagtc aagacaaagt agaagagtac tttcagcagc agctttaaca
    17101 tatgttgcag cagcattggt ttcaatactt caattactta gattgttact aatagctcaa
    17161 agaagaaatg actaaaagta aagtgggttg tttagaaaca accctttttt atgtttaata
    17221 aaagggagaa atttatatgg atgcaagaga agttggattt aaagtattat gtgatataga
    17281 aaaaaatgac aattattcaa atattgcaat aaataagcat tttaaaaatt tagaaataag
    17341 tgatatggat agaggacttg caacagagtt aatttatgga gtaatagaaa ataaatatta
    17401 tttagattat ataatcaata aactttcaaa aataaaggta aaaaaaatgt caacttatgt
    17461 taaaatcttt ttaagaatgg gaacatatca gattttattt ttagatagta tatcagatta
    17521 tgcagcagtt aatgaaactg taaaattatc taagaaatat gataaaaaat cttctggttt
    17581 cataaatgct attcttagaa atgaaataag agctaaagag actataatgg acataactga
    17641 ggaagatagt gtaaaatact tatctataaa gtattcctat aactcttgga ttataaaaaa
    17701 ctggatagat aaatttggac aagaatttgc tgaagatttg ttggaagcaa ataatgaaaa
    17761 gcctagtatc tatataagaa cgaatacact taaaataagt agagaagaac ttattgaaaa
    17821 attaagtgaa atgggtattg tatgtttaaa agttcctatg gtagaagaag ctattaaagt
    17881 cgaaaaaatg aaaaatatag aaaaaaatga attatttaag gctggtttat ttacaattca
    17941 agatataagt tctatgatag tagggaaagt aattaatcca aaagaaggtt catttgtttt
    18001 ggatgtatgt agtgctcctg gagggaaatc tactcactta gctactttaa tgaacaatac
    18061 gggtcaagta gtagctagag atatttttga gcataaacta aagcttataa actctactgt
    18121 aaatagattg gggcttaaaa atgtgtttgt agaaagattt gatgcttctg agattgatga
    18181 aaatagtata agtaaatttg attatgtttt agcagatgtt ccttgctctg gttttggtat
    18241 aataagacgt aaaccagaaa ttaagtataa gaaagaagaa gagttaaaag acatcacttc
    18301 tatacaaaag aaaattttag aaaatgcatc taaatatgtc aaagttggtg gaactttagt
    18361 atatagtaca tgtacaattc aagatgtgga gaatataaat atagttactt cttttgttga
    18421 agaaaatcac aactttgaat ttgttccaat tgatacagta aatgtagact tagacaatca
    18481 agataaagga tatttaaaga tatatcctaa tattcatggt atagatggtt ttttcatagc
    18541 aaaattaaag agaataaggt agtgagagaa tggaaaagaa aaagatagta ttaaaaaatt
    18601 ttactgaaga tgaacttaaa gaatttatga aaactataga tgaaaagccc tttagaggaa
    18661 gtcaaatatt ctcttggata tataagggtg caaaaacttt tgatgatatg aacaatatac
    18721 caaaaagttt gagaaataaa ttagaagagg tttcatgtat agggaatata gatatagaat
    18781 taaagttaga atctaaggta gataatacaa aaaaatattt gtttttatta gatgatggaa
    18841 atataataga aactgtgatg atggattatg atagtagagt tactgtctgt gtttctaacc
    18901 aagtaggttg taggatggga tgtaattttt gtgcatctac aatggatgga ttaattagaa
    18961 acttagagcc atgggaaatt ttagaccaag ttattaaaat tcaagaagat actggtaaga
    19021 gggtatcaaa tcttgtattg atggggagtg gtgagccact tgataacttt gaaaatacaa
    19081 aacaattctt aaaaataatt aacgaaaaaa atggtcttaa tatagggtat agacatataa
    19141 ctctttcaac ttgtggaata gtacctaaaa tgtatgagtt ggcagactta gaaatagcta
    19201 taaatttagc tttatcactt cattcaccat atgatgaaga acgaagaaaa attatgcctg
    19261 tagcaaatgc ttattctatt gaagaaatat tgaatgcatg tagatattat ataaaaaaga
    19321 caaatagaag ggttacattt gaatattctc ttataaaagg agtaaatgac tcagaaaaag
    19381 aagcaaaggc attagctaag ctcctaaaag gtatgctatg tcatgtaaat ttaattccaa
    19441 taaataaagt tgaagaaaga gaatacgaaa agccagataa agcatttatt tataaattta
    19501 gggatagttt ggagaagaac aatatacctg ccacagttag gatgtctatg ggttctgata
    19561 ttagtggagc ttgtggtcaa ttgagaagaa aatacaagta atttgtaagg aggaagtcgt
    19621 atggtttata gttgtgcctc ccatatagga aaaataagaa aaaacaatga agactattgt
    19681 gagggagaag ttatagatac agaacatggt ccaataggaa tatttgccat agctgatgga
    19741 atgggtggac ataaaaaagg tgaagtcgcc agtaaattag cagtagaaaa tataattgat
    19801 tttctaaagg aaaacttatt acaacatgat aatgtaaaaa tagactatat agatgatata
    19861 ttaaaacagg cttataataa tgtaaactca attgtgcaca aaaaatctat ggaagatata
    19921 gaatttgaag gaatgggaac tacattagtt acggctatag tgtataataa tgtcttatat
    19981 gtggcaaatg ttggagatag cagatgttat ttgttaacag atgaaaaatt tgataaaata
    20041 actatagacc attccgtggt tgaagaactt atgatggccc atgtcattac agaagaagag
    20101 gcaagaagac atccacagag aaatagaata actagagcaa ttggaacaga tgatatggtt
    20161 gttgtagata tttttaagaa agaaatcaaa aaaagtgata taatacttct tgcgacagat
    20221 ggattaactg ggtttattga tgatgaagat ataagagatt taatattaga ttatgaatat
    20281 gagagaacta gtaatataag tgaagaattg ataagtatgg ccaatgatgt ttctggtaaa
    20341 gataatgttt cggttatagt gataaaagta taattaggac ggtgatattg tgggagatac
    20401 aattttagga aatcgatatg aaatcatcag gaagattggt gatggaggaa tggcctttgt
    20461 atatgaggct aaagacagat tattaaacag gactgttgca cttaaagtac ttagacctga
    20521 atttgtagat gatgatgaat ttctaacaaa gtttaaaaga gaggcagaag cagtagcaag
    20581 tctttcacat ccaaatatag taaatgttta tgatgttggt gaagatggaa aagttcacta
    20641 cattgtaatg gagtttgtag atgggaagaa tctaaaagag attatacaag atgaaggtat
    20701 attagatgaa tatactgcat tagatataac aaaacaaata gctatggcac ttagtgcagc
    20761 gcacaaaaag gggattatac atagagatat aaaacctcat aatattctca tatcaaatga
    20821 aggaagagta gtaaaagtgg ctgattttgg tatagccaaa gctgtttcaa attcaacaat
    20881 gactaatatt ggtagtataa taggttcagt gcattatttt tcaccagagc aagctaaagg
    20941 taaatttgta actaataatg ctgacctgta ttctctaggt atagttttat atgaaatgtt
    21001 gataggaaaa gttccattta ggggagatag tccaatttct atagcacttc aacacattaa
    21061 tgatgatata gactttacat cagaagaaaa agttaggatt cctcaaagtg taagaactac
    21121 aataaaaaag cttacagaaa aatctagcgc agatagatat caaactgctg aagaattgat
    21181 agaagatata gagtatatag aaaaaaatat tgatttagat tttataaaag aatatgatga
    21241 ttttgcaact aagaaaattg atgaaaaaga aattaataaa gttgtaaatc caacacttgc
    21301 aaaaccagct cctgaaaaag tggttaagcc tgttgaagta gctgatttgg atgatgatga
    21361 agattactat gatgattttt atgaagaaga tgaagaagaa gaagaagaag aggaagagat
    21421 aatgagagct aaaaagaatc agagacctaa gagtactcca agtaagagaa ctaagaaaaa
    21481 gaagaaaaaa caggaaagcc caaaatctag aagaagatta aaagtaattg cggctgtttt
    21541 gatactgata ttgtgtgctc aagtattctt agcatataag tttttatttg ctggagggtt
    21601 tggcaataaa tctttgactg ttcctaatct tgtaaatatg acattagaag aagctcaaag
    21661 tgctgttgaa aaagaaggat tatacctgag tgtaaaaagt gaagagtata atagtgaagt
    21721 tgatgaaaat tgtataatat cacagacacc tgaaggtgga agtacaaatg tcaaaaaggg
    21781 tgatactata aatgttgtcg taagtaaagg ttcaagtcag gcatctgttc ctaatgtggt
    21841 gggacttact ttgagtaatg caaaacaact tattgaagaa aataacttaa aagtgggaac
    21901 tgttaagtat gaatatagct caatatataa agaaggtaca gttttaagtc agagtccagg
    21961 tgctggctca tcaagagctc aggaaggtga tgaggtaaac ctatatgtaa gcaaaggttc
    22021 agaaaaatca aatacacaga ctccaacagt accagataaa aaaccaacaa caccagagaa
    22081 taatacacca acagaacctg gttcaaactc agggaacagt ggtggaaatt caggaagttc
    22141 aaactcagga aatagtggag gaaactcagg caataatgga ggtaactcag gaggttcaaa
    22201 ctcaggaaat agtggaggaa gctcaggagg ctcaaactca ggagataatg gaggaagttc
    22261 aggaggttca aactcaggag ataatggagg aagttcagga ggttcaaact caggagacaa
    22321 tggagatagt tcaggaggct caaactcagg agataatgga ggaacttcat caggaactgg
    22381 cgcaaacatt ggtaaaacag aataaatatt aataaaataa gagctgtcct tttatagaaa
    22441 atattttctt taaagggcag tttgttttgt ataaaatact gaattggtta taatataagg
    22501 agaaaagtat ttatgaagaa gaaaaccgtt ttaattacag gaggagctag agggattggt
    22561 aaagctatgt ctaaagcttt tgcaaaagaa ggttacaatg tattagtaaa ttttaataag
    22621 tcagaaaatg aagctaaaga gttgtatact atattaaatg aaaaaaattt ttctgtaaaa
    22681 ctttttaagg ctgatatttc aaatagagaa gatgtagaga atatggtaga ttattgtata
    22741 aaagaatttg gaggattgga tgtacttgta aacaatgctg gtgtaagtca agataagttg
    22801 tttacagata taaccgatga agattgggat aatatgatga atattaattt aaaaggaagc
    22861 ttttattgtt cgcaagttgc tttaaaatac atgatatcag aaaaaaaagg aaatataatt
    22921 aatatatctt caatctgggg tatatcaggt gcgtcttgtg aagtgcatta ttctataaca
    22981 aaagcaggta taataggtat gacaaaggca ttagctaaag aagtaggacc atcaaatatt
    23041 agagtaaata gcatagctcc aggtgtcatt aatactgaca tgttatctgg atataatgag
    23101 gaagatatag acgctcttgt ggaagaaacc cctctaatga ggctaggaac tccagaagat
    23161 atagcaaatt gtgctatttt cttggcatca gacaaatcaa attttatcac tggtcaggta
    23221 attagtccaa acggaggatt tgttatttaa aaaaaatgtg gtattatata aaaataggaa
    23281 agaataacac gatatactat tcataaacag tatgtgttta tataaatttt ataaagggag
    23341 gcatttgatg ctagaaggaa aaattataaa ggggattagt ggattttatt atgtagatac
    23401 ttataatggt atttatgaat gtaaggctag agggatattt agaaagcaga aaataacacc
    23461 tttggttggt gatagagtca aaataagcat tgttgatgaa gatgaaaaaa agggtatctt
    23521 agaagaaatc gacagtagag atacagaact tataagacct cctattgcaa atgtagataa
    23581 agcattaata gtattcgcaa taaaaaatcc taaaccaaac ttatctcttt tagatagatt
    23641 tatagttttg gcagaaaaag aaaatttaga aactgtaata atccttacaa aagcagattt
    23701 agatgataat gatacattag aaactgttaa aaatatatat gaacttagtg gctataaagt
    23761 cattcctgta agcaatatta ctaagttaaa tatagataaa gtaaaagaag aattaaaaga
    23821 aaatgtagta gtgtttgcag gaccatctgg agttggaaaa tcaagcttat taaatgaaat
    23881 agatgaaaat tttaaacttc aaacaggagt ggtaagtgat aaaataaaaa ggggaaaaca
    23941 tactactcgt cacgctgaac ttttaaaatt ggaatttggt ggaatggttg cagatacacc
    24001 aggttttagt tcccttgcac ttgaagatat agaggaagtt gaacttaaag attattttat
    24061 agaatttgat aaatttaatg attgtaagtt tggttcaaag tgtattcatg aaaatgaacc
    24121 taattgtgct ataaaagaag ctgttacaaa tggagaaata tctaaagaaa gatatgatag
    24181 ttatatacag ctgctacatg aaataagaca aaataatagt aggaggtatt agaaaaatga
    24241 ttaaattagc accatcaata ttatcagcag attttgcaaa attattagaa gatgtaagaa
    24301 aagtagaaag tgcaggatgt gaatatcttc atatcgatgt aatggatgga cactttgtac
    24361 caaacattac attaggacca cttgtagtca aaagtttaaa gaaagaaaat ataaatatgg
    24421 tatttgatgc tcatcttatg atagaaaatc cagaccagta tattgaagaa tttgtaaaag
    24481 caggatgtga tataataact gttcaccagg aggcttgtgt tcatctacat agaactatac
    24541 aaaatataaa atcacatgga ataaaagctg gtgtagtact taatccagct acaccagtag
    24601 atacgattaa acatgtacta cctgatttag atatggtatt attaatgtct gttaaccctg
    24661 gatttggagg acaatcattt attccttgtg ttttagataa gattaaagag ttaaaagcta
    24721 ttatagatag tcaaggatta aacattgata tagaagttga tggtggtata agccctaaaa
    24781 acgtagccga agttgtacaa gcaggagcta atgtaatagt agcaggttct gcaatatttg
    24841 gaagtgatga cattcaagaa acagttaatt tatttagaaa gaatgcttct cttgaagaat
    24901 tagtttaaat ttaataatga aaatctgtat tgttttaaat ggcgaaattg aagattacga
    24961 agttacaaga gatattataa ttaaggaatg ttatgactgc ataatatgtg cagatggagg
    25021 ggcaaatcac acttataaaa tggaaataat gccagattat atattaggag atttagattc
    25081 agtagaagaa gaaaagataa acttctataa aaataaaggt gttaaatttg aaaaatttcc
    25141 atctaaaaaa gatgagactg atacagagct ttgtcttttt ttagctaaga cacttaaagc
    25201 aaatcatatt gatttttttg gtgctttagg gggaaggatt gaccacactc ttgcaaatat
    25261 aaagctatta tattatttaa aagaagatgg tatatattct aggatacttt ctgataaaga
    25321 agagatgtat atagttgaaa atgaggaaat ttcattgtat ggaaacccag gggacactgt
    25381 ttctgtaaca gctataaaag gtgatgctaa gggtgtaaca cttactggct tagaataccc
    25441 tctcgataat tattatatga aatactctgt acctattgga atatcaaatg ttatgttgag
    25501 taattcttgt aagattaaag tagagcaagg ctgtgtactt gtagttagaa atttataaaa
    25561 tttgattttg acaattggtt ttatattgta gagcagatac aatcaagtaa aatatattta
    25621 tgattgtatc tgctttttag tatataatta taaatatata ataaactttg acataagggg
    25681 gattttttgt ggtagatagt attatttttg atttagatgg aacattgtgg gattcaactg
    25741 aagaagtatg caaagtctgg caggatgttt taagtaaaca taaggaaata gaattaagtg
    25801 tcacaaaaga tttatttaga agtcttatgg ggctttcttt tgaggaaata gcaaatcgtt
    25861 tatttccaaa tttaagtgaa gaagaaagaa tgaatttatt gaatgaatgt tctttgaaag
    25921 aatgtgacta tttatctgaa aatggagcta aattatttga tgatatagaa gatactctta
    25981 aattactatc taaaaaatat aaattattta tagtaagtaa ttgtcaagct ggatatatag
    26041 aatctttttt aaaagcacat aaactagaac agtattttat agattttgaa tgtcctggta
    26101 atactggttt gcataaagga gaaaataata aacttataat tgaaagaaat aaattaataa
    26161 atccaatcta tgttggtgat acgcaaggtg atgcaaatag tgctaagttt gtaggtatac
    26221 cttttatata tgccaaatat gggtttggga atgtagatgt atatgattat tttatagatt
    26281 catttaaaga tttgttagaa catgatatat taaaataaga tgagtttaaa aattatatta
    26341 aaataataaa aaagttatgg ggggatttaa aaatggaatt acaagatact atttttaaaa
    26401 gacaaagtgt aagaaagttt aaaaatcaag atgtatcaga tgaagatatt ttaaaaatga
    26461 taaaagcagc aggtgcagca ccatcaggca agaatattca aaactggcat tttgttgtta
    26521 taaaacgccg tgatttaatg gaaaagatag cagatgtgat aactaaaaag caacaagaaa
    26581 tacttgtgga aatggataaa gtatcagtgg ataaggctaa tagatttaga aaatttgtta
    26641 aaaactttac actattttac ttaaaagctc cagttttagt attagttttt acaaaggtat
    26701 ataatccatc aggatattat gaattagagc ttatagatgc acctaaggag actatagata
    26761 aattatttat aagaaatcca ggtatgcaga gtctaggtgc ttcaattgaa aactttacac
    26821 tatcagcaat tgaacttgga tatggttctt gttggttgac aagtcaaaac tatgcagcag
    26881 atgagataga agctgtttta gaagcagaaa ctggttttga aaagggagag tattttttag
    26941 gtgcaatgtt agctcttgga gtgccagagg acaatttgaa gagtccatct aaaaaaccag
    27001 tagaagaaat atgtacattt ataaaataaa agtcaaggac attttaaaat caaa
//
LOCUS       DAEPOZ010000028        25209 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.028, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000028 DAEPOZ010000000
VERSION     DAEPOZ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25209
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.028"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(9..1607)
                     /locus_tag="KR440_000956"
     CDS             complement(9..1607)
                     /locus_tag="KR440_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH0698017.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELNISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINILESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIIW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
     gene            1935..2111
                     /locus_tag="KR440_000957"
     CDS             1935..2111
                     /locus_tag="KR440_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698018.1"
                     /translation="MEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNEAF
                     SILEKELNISKANT"
     gene            2285..3415
                     /locus_tag="KR440_000958"
     CDS             2285..3415
                     /locus_tag="KR440_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBH0698019.1"
                     /translation="MNNFEIKTRIKFGEGSLKVLQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKSIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSDTAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILNNIK"
     gene            3656..4006
                     /locus_tag="KR440_000959"
     CDS             3656..4006
                     /locus_tag="KR440_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBH0698020.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKRD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            4014..4463
                     /locus_tag="KR440_000960"
     CDS             4014..4463
                     /locus_tag="KR440_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HBH0698021.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDDIGLEKLFKYLEKSKNIDEV"
     gene            4565..5140
                     /locus_tag="KR440_000961"
     CDS             4565..5140
                     /locus_tag="KR440_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBH0698022.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENLAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            5133..6542
                     /locus_tag="KR440_000962"
     CDS             5133..6542
                     /locus_tag="KR440_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HBH0698023.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSNENGTEVLITIPTQINI"
     gene            6726..8159
                     /gene="eutA"
                     /locus_tag="KR440_000963"
     CDS             6726..8159
                     /gene="eutA"
                     /locus_tag="KR440_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBH0698024.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDENIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQTIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSVDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            8177..9541
                     /locus_tag="KR440_000964"
     CDS             8177..9541
                     /locus_tag="KR440_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HBH0698025.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFYKDGKLTELAGDAS
                     VLLG"
     gene            9554..10435
                     /gene="eutC"
                     /locus_tag="KR440_000965"
     CDS             9554..10435
                     /gene="eutC"
                     /locus_tag="KR440_000965"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HBH0698026.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVKKTTEVVVDNNAFI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSQETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            10455..11108
                     /gene="eutL"
                     /locus_tag="KR440_000966"
     CDS             10455..11108
                     /gene="eutL"
                     /locus_tag="KR440_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBH0698027.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEIAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            11119..11820
                     /locus_tag="KR440_000967"
     CDS             11119..11820
                     /locus_tag="KR440_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBH0698028.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRIDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEITDNVSKNLSEKENVILDVNENSGDTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            11823..13292
                     /locus_tag="KR440_000968"
     CDS             11823..13292
                     /locus_tag="KR440_000968"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HBH0698029.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISE
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNIPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            13388..13675
                     /gene="eutM"
                     /locus_tag="KR440_000969"
     CDS             13388..13675
                     /gene="eutM"
                     /locus_tag="KR440_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HBH0698030.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            13802..14563
                     /locus_tag="KR440_000970"
     CDS             13802..14563
                     /locus_tag="KR440_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HBH0698031.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYKYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLEDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            14576..15205
                     /locus_tag="KR440_000971"
     CDS             14576..15205
                     /locus_tag="KR440_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBH0698032.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            15247..15972
                     /locus_tag="KR440_000972"
     CDS             15247..15972
                     /locus_tag="KR440_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HBH0698033.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDEFEILEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMKGKKVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDVLNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKL"
     gene            15985..16257
                     /locus_tag="KR440_000973"
     CDS             15985..16257
                     /locus_tag="KR440_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HBH0698034.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            16250..16801
                     /locus_tag="KR440_000974"
     CDS             16250..16801
                     /locus_tag="KR440_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBH0698035.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKVCAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYVVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            16813..17904
                     /gene="eutH"
                     /locus_tag="KR440_000975"
     CDS             16813..17904
                     /gene="eutH"
                     /locus_tag="KR440_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBH0698036.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NSGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLRKENV"
     gene            17906..18379
                     /locus_tag="KR440_000976"
     CDS             17906..18379
                     /locus_tag="KR440_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HBH0698037.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            complement(18499..18600)
                     /locus_tag="KR440_000977"
                     /pseudo
     CDS             complement(18499..18600)
                     /locus_tag="KR440_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            18895..19416
                     /locus_tag="KR440_000978"
     CDS             18895..19416
                     /locus_tag="KR440_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0698038.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
     gene            19795..20970
                     /locus_tag="KR440_000979"
     CDS             19795..20970
                     /locus_tag="KR440_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0698039.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQAYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDTLKLGCEFMAKYAVEFLNKNNN"
     gene            21005..22543
                     /locus_tag="KR440_000980"
     CDS             21005..22543
                     /locus_tag="KR440_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBH0698040.1"
                     /translation="MQGALKENKKKSGGGFLGWIERVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQVI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            22575..23777
                     /locus_tag="KR440_000981"
     CDS             22575..23777
                     /locus_tag="KR440_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH0698041.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFIQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     SEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQEKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     IWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
     gene            complement(23956..24555)
                     /locus_tag="KR440_000982"
     CDS             complement(23956..24555)
                     /locus_tag="KR440_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0698042.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEARCIKVQL"
     gene            24724..>25209
                     /locus_tag="KR440_000983"
     CDS             24724..>25209
                     /locus_tag="KR440_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698043.1"
                     /translation="MSNIITNLIESVFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FS"
BASE COUNT        10031 a         2969 c         4455 g         7754 t
ORIGIN      
        1 aagtgcaact aattgcttaa agaaacagaa ttatccactt cagcacccat ataaacaata
       61 ttaagatttc ctaaactgct atcccaaatg atactattaa taatagtaga taataacaat
      121 ctcttatcat ctaaactggc attatcaact tcatcattaa atctcttcaa attactaaca
      181 ataatatcaa tattaatact gtcactacta atattgatat tactagattc caaaatatta
      241 atcttatcct ttaaaatact aatctcatta tttaggctct caatctggga aactatataa
      301 tcagaagcag tactaccagt attttttgca agctctataa ctaaattttt aataagtctt
      361 tccttatcta aaatttgatt tttaagtaaa ttagtctcag atttaaaaat attaaaaaca
      421 ttatcagtct taatgttatt actattgaat gcacttataa tagactcttc tttacaattc
      481 ttaattttat ctataactaa actttcaact aatggaccat taagattttt acagttgcaa
      541 ctatcagacc caagtgtttt tttagtacca caaatataat aatactttaa atccttgtca
      601 gtttttttgc ctttataggt aattctcata ttagaaccac atttaccaca ttttataaga
      661 ccagataaaa gagcagtttt tccagttgat atccttggag ttttatcacg atttttatct
      721 aattgatttt gtacttcaag ccatatttca ggttctatga ctccatggtg ttttgcaatt
      781 gctgctattg agttagatgt atttttagca taggtaagga ttccacattt tctatctgga
      841 gtacctacaa catccatacc actattttta aggtaatcta aaatttcatc attagctttt
      901 acatatgctg gatttctaag tattctagat aaagaactag ggtcaaattt accaccacga
      961 ggacccctta ttccattatt atagacatat ttatataact tagataagga cctaagttct
     1021 aaatatttat tgtatattag ctttacaatc tccatttgtt catcatttac ttgaagctta
     1081 tacatacttc tttcttttag attttcatct aagaatgtga ttttttcact gttgaatcca
     1141 tgaggtgtat tgcctccaag ccatctacca ctacgggcta attcatacat gttgtctttt
     1201 atacgttctg ctattgtttc tctttctaat tgagcaaata cacttgatat aaacatcata
     1261 gctcttccca ttggagttga agtatcaaat tgttctttga tagatataaa tgatatgttt
     1321 agttcattta atttttctat taaagtagag aagtcagata catttctcga tatcctatct
     1381 aatctataac aaactaggta actaaatttc ttatctttag catcctgcat cattttctta
     1441 aattgtggtc tatctatatt gctaccacta aatccctcat cttcatatat caaaaaatct
     1501 tttacaccaa tagactttgc ataatcaata cataattgta tttgattttc tatagaatca
     1561 ccttttccag taaatcttga ttttcttgaa taaattgcag ctttcaaaat taaaacctcc
     1621 tataaattag gatatatatt tgaaattatt tatatacaac cttttaagta aacttattag
     1681 aataaattta ttaattaggt aaatttatat aattctactt tttataatat atgactattt
     1741 attttatttc tttactattc aagttctaaa tcaataataa attttgatat ttaattgtat
     1801 caggacaaat ctctaatttc atatattatt aatataaaat cataggttag tgtgaaaaag
     1861 ttgaaattta aatattttaa aagataattt tttggaacta ataggtataa caaaaattta
     1921 tagggggatt gcatatggaa atcaatgtta tagaaatttt ccctaaagat aaagctaaac
     1981 ttaataaaat agaaatggat aaagctagtt ggtttgtaaa tataataagt aaaaaatatc
     2041 ctaaagaagc tttaaatgaa gcatttagta ttttagaaaa agaattaaat ataagtaaag
     2101 ctaatacata agctttgatt attgatttta tatactattg gttttatacg ctatgacttg
     2161 acattatatt aatcaaatag tatattttta aatatagcac aatgaagtgt taaaagagca
     2221 atggagctcc atagataaag tctatggagc ttttttgcgt atttaactta aggaggatag
     2281 agctttgaat aactttgaga ttaaaactag aataaaattt ggagaaggtt cactaaaagt
     2341 tttgcaagaa ttaaaaaaca aaaatgtatt aataataaca gaccctttta tggtgaaatc
     2401 aaaaacaata gataaaataa catctaattt aataaactca acatatcaag tttttgaaga
     2461 aatagtacca gacccaccaa tagaagtagt agtagcagga atagaagcat ttaaatctat
     2521 aaatccagac gtaataatag cattaggtgg tggttctgca atagatgcag caaaatctat
     2581 aatggacttt tctaaaaaaa tattaaaaat taacgaagta gagtttatag caataccaac
     2641 aacaagtgga acaggttcag aagtaacatc atttgctgta ataacagata gtcaaaaaaa
     2701 atgtaagtat ccattagtga gtgatgattt acttccagat atggcaatac tagacccaga
     2761 gcttgtaaaa actgtaccaa attttataac agcagataca gggatggatg tattgacaca
     2821 tgctatagaa gcttacgtat ctacaaattc taatgacttt tcagatgcac tagctgaaaa
     2881 atctataaag ttagtattta aatacttaat aaaagcatat aaagatggaa atgacataga
     2941 agctcgagaa aaaatgcaca atgcatcttg tttagcaggt atggcattta accaagcatc
     3001 acttggcata aatcattcta ttgcacatgt attaggtgga aaattccata taccacatgg
     3061 aagaacaaat tctatattac ttcctcatgt aatagaatat aattcatcaa tagtaggata
     3121 tactaacaca acttactctg atacagctaa aaaatatgca caaatagcag aattagtagg
     3181 acttgggagt gctaatataa gaatatctgt tagaaattta gtaaatgaaa taaagaaact
     3241 acaaaaagaa atgaatatgc caactaaatt gacacaatgc aaattaagtt tagaagatat
     3301 aactaatgaa gaaagagaaa tagcaaaact tgctataaaa gatacttgta cattaactaa
     3361 tcctaaggta ccaactgaag atgatataat aaacatatta aataacatta aataactaca
     3421 ttttattaca tgtaaatata atattataca aatagggaaa atgtttttaa aaatataaat
     3481 tatatattga caaatataaa acataaaact ataatgaaag tatcacaaag gcgtgatact
     3541 ttagtagaaa gcaatggagc ttcttaggaa tattaatatt attcttatgg agctcctttt
     3601 attttaagca aatagctttt taataaacta tattcaaaga aagcagggaa taaatttgac
     3661 tgaagaatct aagcaaagag ttattcaaga atatgttcct ggaaaacagg ttacattagc
     3721 acatatcata gctaacccta atgaagacat atataagaag ttaggattag tacttgataa
     3781 aagagatgca ataggaatac ttacaataac accaagtgaa gcgtcaataa tagcagcaga
     3841 tgttgctaca aaggcttcaa atgtatcttt aggttttata gatagattta gtggttcagt
     3901 agtaatatca ggtgatgtaa gttctgtaga atcagcgtta aatgatgttt tagaagtctt
     3961 aggaaatatg ttaaacttct catctacaaa aatcacaagg acataataat acaatgaaga
     4021 atataatatt tatgggaaag acaggtagtg gtaaaactac cttatgtcaa aaactagacc
     4081 agttagaatt aaagtataaa aaaactcaat cagtggaact ttataataat gcaatagata
     4141 ctcctggtga gtatatggaa aatagaaacc tatataatgc cttaataaca acatcagcag
     4201 atgctaaaat tatagcaata gtgtatgatg ccacacaaga agaaaattat atagcaccag
     4261 gttttgccag tatgttttgc aaagacgtaa taggaattat aactaaaata aataaagcta
     4321 ctgaaaacga agtcaatata gcttatgaaa gactatcact agcaggagca agtcaaatct
     4381 ttaaaataga cacagttgat gatattggac ttgaaaaatt atttaaatat ctagaaaaat
     4441 ctaaaaatat agatgaagtt taaggggaaa tattttttta ttcaaaaata ttattaaagt
     4501 atgataaaat aaaaaaatgt ataaactatt ttaagaagat ttaatcttta agcgaggtag
     4561 gaacatgggg aaaaaaatac tattggttga tgatgaacca ctgttaagga tggatacaaa
     4621 agatatatta gaaagtcatg gctatgaaat aataggcgaa gcaagtgatg gatttgaagc
     4681 tgttgaaatg tgtaaaaaac acaaacctga tttagttata atggatatag agatgccaat
     4741 acttgatggt ataaaggcag gcaaaataat atgtaaagaa aatctagctg gtggagtact
     4801 tttattaact tcatttgaag ataaagaata cgtagaaaag gctaaaagta taggtgctta
     4861 tggatattta gtaaaaacag caagtgaaaa atcacttatc ccaaccatag aaatgtgttt
     4921 aaataaggta aatgaatttg aaaaaatgaa aaaagattta gaaaaagtaa attctaaatt
     4981 gaatgaaaga aaattagtag aaagagcaaa aggtatactt ataagagagt ttaagatttc
     5041 tgaggatgaa gcatacacta gaataagaaa attaagtatg gataaaagaa ctacaatgat
     5101 ggaaattgcc aaaatgatta taataggata cgatgactaa tacgataaaa aaaatatcaa
     5161 ctgcacaaac atacttaaat aataacgaaa ttgaaaaaat tatagaagta tcaaaatcct
     5221 tagatttaat ggcttctttt tatgaggctg atgtttttat agatgtacta agtaaatttg
     5281 atgatgaagc aattgttgtg gcacatggta gacctaaaga aaagtcaatg tactgtgaaa
     5341 atgttatagg taaaaaggct ttaattgaaa atgaaccagg agttataaag tgcttaaaaa
     5401 caggagaaag agtaagagat attaaagctt taacacagga atataaactg gtcaaacaaa
     5461 atattcagcc aataacacta gatgacaaag ttttagcagt actaatagtt gaaaaagata
     5521 taagtagtga agttaagaat gagtttaatg ctagtgaaga cgaagaaaat aaaaatttac
     5581 atcagtttat aaaacttatc ggaaataatg actttttagt tgataattta aattcagcca
     5641 tattaatatt tgataaaaat ggaaaattaa aaattaaaaa tcgtattgca actgagatat
     5701 acaaaaatct tgggtttgga tatgatatac aagatatgca ttacaatgat ttagatatag
     5761 aaaccaacac ctttgaaaat atattaatgg atgaaaatta tgatgagaca aatgaagttt
     5821 caataggtaa tatgtttttt aaaataaaga ccatatacat caaagatgat gaatttaagg
     5881 tttgcaaaat agtacaagat ataagtgatt taaagagaaa agaagaagaa ttagttttaa
     5941 aaacagtggc tataagagaa gctcatcata gagtaaaaaa taatcttcat acagtaatat
     6001 ctataataaa aaaacaaagt agattatctc aaaatgatga agtaaagcag tgtttagata
     6061 acataataaa cagagtattt gcaatacttt caactcatta tttactatct aaagaagttg
     6121 ataataatat atctatacga gaaggtatca atttattagt gtcaaatatt caaggtggct
     6181 atttatttag taaggatata gatatacgta taactggtga agattttaaa attagtggag
     6241 aaaaagcaac agcaatactt ttggttataa atgaaatact acaaaattgt tatgaccatg
     6301 cattttcgga tagagaacat ggtactatac aaatattaat aaataaagat aacgactata
     6361 gaaatattgc tattatagat gatggaattg gatttgatag taataatata aataaaaata
     6421 gcttagggac atatataata gatagttata taacacagat gttaaaagga aatatagaga
     6481 gaaaaagtaa tgagaatggt acagaagtat taattactat acctacacaa attaatattt
     6541 aaataataac tcatctacaa agtagtagtt ttaattgagc aaagaggctt aaggttatta
     6601 atagattaag ttaattgaat gtatttataa aaattataag tatatattca atttaacagg
     6661 tatttattat aactttaagc ctctttgctt gtttaagaca tacttaaatt ttaggaggtg
     6721 agaaaatgag agaagagtta ttaagtgttg gtattgacat tggaacaagt actactcagt
     6781 taattttttc taaattaata gtagaaaata ctgaatcaaa tttttcggta cctagaataa
     6841 gtatagtaga caaagaaatt atttataaaa gcaaagttta tttcacccct cttatttcaa
     6901 ataatgaaat aaatggtcaa caaataaaag atatagttga aaatgaatat aaaaaagcaa
     6961 atatatctaa agaggatata catacaggag cagtaataat aactggtgaa acagctagaa
     7021 aagaaaatgc aaatgatgta cttcatactt tgagtggatt tgctggggac ttcgttgttg
     7081 caacagcagg acctgattta gaaagtataa tatcaggaaa aggagctgga gctcatattt
     7141 attcaaaaga acattcaact acaattgtaa atatagacat aggtggggga actagtaaca
     7201 tagcctcatt taaaagggga gaagtaaccg atactgggtg tttggatata ggtggaagac
     7261 ttgtgaagat agataaaata agtaaaaaaa taacttatat atcacctaaa atagataaaa
     7321 tcataaaaga taataattta aatcttcaag taggcacaat ggctactgta gaaaatacaa
     7381 agccaatagt ggatataatg gttgacttat taatggaaag cataggatta aagaaacaga
     7441 cacctttgtt tgattttata ataaccaata aatcaataag tatagatgaa aatattaaaa
     7501 atgtttcatt ctctggtgga gtagctgatt atatttacta tgatggagaa ataaaagatt
     7561 attttgaata tggtgacttg ggaattttac ttgggcaaac aataaaaaat tgtgaatacc
     7621 ttaaaaaagt aaatgtagtt aaatctatgg aaacaataag agccacagtg gttggggcag
     7681 gttctcatac tactgaagta agtggaagta caataactta cactagcgaa aagtttccac
     7741 ttaagaactt gccagtttta aaattatcag ttgatgaaga aacaggtact tcaaaacaaa
     7801 tcagtgaagc tataaaaaat aaacttaatt ggtttaaact agaaaatgat ttgcagaaag
     7861 ttgctatagc catgatagga aagaaaaatc caaccttttt agatatacaa gagtatgcta
     7921 aagggtttgt agatggaatg caacaaatca tagaaaatga tttacctttt atagtaattg
     7981 ttgaaaatga tatggcaaaa gtgttaggtc aagcaatata tgcattatta aattatgaaa
     8041 aagaaattgt ttgtatagac agtgtcaagg ttgaaaatgg agactatatc gacattggaa
     8101 aacctatagt tgaaggtaaa gttttacctg tggtaataaa aacattagtt tttaattaat
     8161 agaaggaggt aagtaaatga ttttaaatac aaaattattt ggacgtactt atcagtttaa
     8221 aggcttatat gatgtaatgg ctaaagctaa tgaagaaaaa tcaggagata aattagcagg
     8281 tcttgcagca gaatcagctg aagaaagaat agcagcaaaa gttgttttat cacacataaa
     8341 attggaggat ataagaaata atcctgcagt ggcatatgaa gaagatgaag taacaagaat
     8401 aatacaagat gctgtaaatg aaaaagttta tgaagaaata aaaaattgga cagtatctga
     8461 acttagagaa tggctattgg attcaaatac tacaagtctt gatataagaa gaatatcaag
     8521 aggattaact tctgaaatga tagcagcagt tgcaaaatta atgtcaaata tggacttagt
     8581 ttatgcagct aaaaagataa aggttactgc tcactgtaat acaactatag gagaacaagg
     8641 aacattatca gcaagacttc aacctaatca tccaacagac gacccagatg gaatacttgc
     8701 atcacttctt gaaggtttaa cttttggtat aggagatgca gtacttggat taaatccagt
     8761 tgatgactct gttgagagcg ttactaaagt attaaaaaga tttgaagaag taaaacaaaa
     8821 atatcagata ccaactcaaa catgtgtatt ggcacacgtt actacacaaa tggaagcaat
     8881 aagaaatggt gctcctgcag atttaatatt ccaatctata gcaggttctg aaaaaggaaa
     8941 tgaagcattt ggatttaatg gcgacactat agaagaagct agacagttag ctttaaaaca
     9001 cggtacagct acaggaccta acgttatgta ttttgaaact ggacaaggtt cagaattatc
     9061 ttcagaagca catcatggaa tagaccaagt tactatggaa gctagatgct atggatttgc
     9121 taaaaagtat gaaccattct tagtaaatac agttgttggg tttataggac ctgagtactt
     9181 atatgatgct agagaagttg taagagcagg acttgaagac cactttatgg gtaaactaca
     9241 tggcttacca atgggtgttg atgtttgtta cactaaccat atgaaggctg accaaaatga
     9301 tatggaaaac ttagccatac tattaacaac agcaggatgt acgtatttta tgggaatacc
     9361 tcatggagat gatgttatgc ttaactatca aacaacagga taccatgaaa cagcatcact
     9421 tagagaaatg tttggactaa cagcaataaa agaatttgat gcttggatgg aaaagatggg
     9481 attctataaa gatggtaagt taacagagtt ggcgggagac gcatctgtat tattaggata
     9541 ggaggtattt aatatgaatg agaaggattt aaaagcattg gtagaacaat tagttggaca
     9601 aatggttgga gaattagata ctaatgttgt atctgagact gttaaaaaga caactgaagt
     9661 tgtagtagac aacaatgctt ttatagatga tataacagaa gttgatataa gaaagcaatt
     9721 actagtaaaa aatcctaagg atgcagaagc ttatttagat atgaaagcaa aaacacctgc
     9781 aagattaggt ataggaagag ctggtactag atataaaaca gaaacagttt taagatttag
     9841 agcagaccat gctgctgcac aagatgctgt attctcttat gtagatgaag aatttataaa
     9901 agaaaataat atgtttgcag tggaaacttt atgtaaagat aaagatgaat acttaacaag
     9961 accagatttg ggaagaaaat tttctcaaga aactataaat aatataaaat ctaaatttgg
    10021 tacaaatcaa aaggttttaa tattagttgg agatgggctt agctcagctg caatagaggc
    10081 aaatttaaaa gactgtgtgc cagctataaa acagggtcta aaaatgtatg gtatagattc
    10141 aagtgaaatt ttatttgtta aacattgtag agttggtgct atggaccact taggtgaaga
    10201 attaggttgt gaagttatat gtatgttggt tggagaaaga ccaggattag ttactgctga
    10261 atcaatgtca gcatatatag cttataagcc atatatagga atggcagaag caaaaagaac
    10321 agttatatca aatattcaca aaggtggaac aactgcagtt gaagcaggtg ctcatatagc
    10381 tgaacttata aaaactatgt tagataaaaa agcttcagga atagacctta aataaagtcg
    10441 agggagtgta gaatatgaaa aatgatgtaa ttcgtcctaa tatactagga attaaaataa
    10501 tttcgaatat aagtccagaa atggcacaaa aactagaatt aaaatcacat cacaaaagtt
    10561 taggtttaat aactgcagac tgtgatgatg ttacgtatac agctcttgac gaagcaacta
    10621 aagcttcaga ggttgatgtt gtatatgcaa aatcaatgta tgcaggtgct gcaaatgctt
    10681 caacaaaact tgctggtgaa gtattaggaa taatagcagg cccaagccca gcagaagtaa
    10741 gaagtggtct aaatgcagta gttgattttc tagagtatgg agcaactttt ataagtgcaa
    10801 atgatgatga ttcaatagca tactatgctc actgcgtatc aagaacaggt acataccttt
    10861 cagagatagc aggaataaga gaaggtgaag cattagctta tttagtagca ccaccacttg
    10921 aagcaatgta tgcactagat gcagcaatga aagctgcaga tgttaagatg tgtgaattat
    10981 ttgcacctcc aactgaaaca aactttggtg gagcattact tacaggttct caatcggctt
    11041 gtaaagcagc atgtgatgca tttgcagaag cagtaaaatc agttgcagac aatccaacag
    11101 gattttaagg atagttagat ggatgctata ggcttaatag aagtaatagg atatgttgct
    11161 gcaatagaag cttctgatgc ttgtttaaaa tctgcaaatg ttaatattgt aagaattgat
    11221 aaggttgggg caggtattgt tactttaaca atatgtggag atgtcggtgc tgtaaaagct
    11281 gctttagaag caggtgaaat agcagcttcc agagttggta cattaagaac atcacatatt
    11341 ataccgagaa tccataatga agtaacaaat gctttattca aaactaaaga acctaaagtc
    11401 tgtgaagttt cagaagataa agaaaaaaac tttgagatta cattggaaat aactgataat
    11461 gtatcgaaaa atttatcaga aaaagaaaat gtaattttag atgtcaatga aaacagtgga
    11521 gatactgaaa atttatatag tgatatagat aaaacaaaca aaaaaattga aacagttgat
    11581 gatagtttaa cagaagctaa tgaggaaaaa atagaaaata ctaatgataa agaagtattg
    11641 gaacaagtta aaatagaaaa ttccgatttt gatagagatg acctatcaaa gaacagtgta
    11701 aaaaaactta aagctatggc taagaaatta gattcttcaa taacttataa agatttaaat
    11761 gttcttaaaa aagaagaatt aataaactta gttaaaaagc ttagcagaag ggatagatag
    11821 ttatgacact tttagataaa gatttagtat ctatacaaga agtcagaact cttgtaagaa
    11881 gatctaaagt ggctcaagag aaactttcta aaatgagtca agagcaaata gataaaatag
    11941 taaaagctgt tgtagatgca gcatttaata atgctactaa attagctaaa atggcaaatg
    12001 aagaaactgg atttggaaaa tgggaagata aagtaattaa aaatactttt gcatctatgg
    12061 gagtttatga aactttaaag aacacaaaaa ctattggaat aattggtgaa gataaagaag
    12121 ccaatacgat agatatagct acaccagttg gagttgtggc aggtcttata ccttcaacaa
    12181 acccaacatc aacagtaata tacaaaacta taatatctct aaaagcagga aatactatag
    12241 ttttctctcc acatccaaat gcaaaaaact gcatacttga aacagttaat ataataagtg
    12301 aggctgctat aaaggctgga gctccagagg attcaatatt atgtataact aatccaagta
    12361 tagttggaac caatgaattg atgaaacata aagatacaaa cttaatacta gcaactggag
    12421 gagaagcaat ggttagagct gcttactctt caggaacacc agctattgga gttggaccag
    12481 gaaatggacc agcatttata gataaatcag caaatgttaa attagcaatc aaaagaataa
    12541 tagattctaa aacttttgat aatggtgtta tatgtgcatc agagcaatct atagtaatag
    12601 aaaaatctat ggaagaaact gttttaaaag agcttaaaaa tcaaggtgca tatgttttaa
    12661 atgaagagga aagtgaaaga ttagcaaaat tcatattaag agctaatggt actatgaatc
    12721 cacaaatagt tggtaaaagt gttgaggtaa tagcaaattt agcagactta acaaatatac
    12781 ctaaaaatac aaaagttcta gttgcaagag aaaataaagt tggtcacaat gtagcctatt
    12841 caagagaaaa acttacacca atactagcgt tttatgttga agaaaatgta gaagctatta
    12901 tgaacagatg tagagaaata ttgttaaatg aaggtgcagg tcatacattc tcaatgcatg
    12961 taaatgatga agaattggta aaaagatttg cccttaatat gccagtatca agaatacttg
    13021 tgaatactcc ttcagcactt ggaggaatag gtgcatcaac aaaccttgta ccagcactaa
    13081 cacttggttg tggtgcaata ggtggaagtt caacttcaaa caatatagga ccacttgatt
    13141 taataaatgt taaaagagta gcttatggaa tcaaagaaat agaagaactt ggaagagata
    13201 aatctcaagt tgaaaaatca atgtttgact caatggataa agaagaatta ataaatcttc
    13261 ttgttcaaaa actagtcaaa gaactagcat aactaaaata aacaatgaaa cttatggcta
    13321 tcgctataaa tttataaaaa tataaactat aaaaaactat aaaattaaat ttttggagga
    13381 taagattatg gcaagtgcaa acgcattagg aatgatagaa actaaaggat tagtaggagc
    13441 aatagaagca gcagatgcaa tggttaaagc tgcaaatgta caattagtag gaaaagagca
    13501 agtaggtgga ggattagtta ctgtaatggt aagaggagat gttggagctg ttaaagctgc
    13561 tacagatgca ggagctgcag ctgccgagag agttggagaa ctaatatcag ttcatgttat
    13621 accaagacca cactttgaag tagatgctat attaccaaag gtatcagctg agtaatataa
    13681 gaataatttt aaaatttaaa ttttgattga aaaagaggct atctcaaaaa tgagtcttac
    13741 taataaactc aatgtatttt gaggtagtct tttttataca tctaatagga gatgtgataa
    13801 aatgggtgtt ttaacagaaa acgaagtaaa aagaaaaatt ggaaaaaaca aaaataatat
    13861 aactgaattt tatatagaaa aaggtcaaat aataactcct tctgcaaaat cttatttagc
    13921 tgagagaagt attgagctta aatatgttga taaactttca gatgtaaaca atgaacctaa
    13981 agaagttgta aaagaagtag ttaaagagaa aaaatataaa tatactactg tatttggcgc
    14041 taaattggat gaaaaaccag aacatatgac tcaattaaat ggaaatcttt tagtttttaa
    14101 agaccataaa agaataatat tgagaggaaa aatagattct cttgaatcag aaatactaaa
    14161 ggtacagata ctttgtcaga aactagatat gccaaagctt gttaaggaat tgcaagaagt
    14221 attagacttt gtaagaaata taatgagatg tgaagtttta gatgaaaagc tagaagactt
    14281 taaactttta ggattaactc ctcaagaact tagagaacaa tctcactatc ctaaaaaata
    14341 ctttggtata ggacatgaat tcccaagtta tgaaatggga gaagtagtag tagctataaa
    14401 tagtataaga agtaatacac gagaggttga acttgcagcc tatgaagcat ttaagggtga
    14461 gtatggaaat gttcacagag aagatttaat aaaggcactt aatagattat cgtcagtatt
    14521 ttggataatg atatataagg ttagaactaa taagtataag tagttggagg acataatgga
    14581 gaatctatta aatgaaatag tggaagaagt aataactaga gttaaaaaag aagcatttat
    14641 agaagttgaa gcatcaggaa gacacgttca cttaagtaga gaagatgtcg ataaattatt
    14701 tggagaagga tatactctta caaaactaaa agacctatct caaccaggtc aatatgcatg
    14761 taaggaaaga gttacaataa ctggtcctaa aggaagtata aaaaatgtag ttgtacttgg
    14821 accatgtaga aatgaaactc aggtagaaat atctttaaca gatggttcaa ctcttggact
    14881 aaaagctcct ataaaacaaa gtggagactt agaaggtact ttaagtatta aaatatcaac
    14941 acaatatggt gaagttgaac ttgataaggg gttaatggtt gctaaaagac atatacacat
    15001 gactcctaaa gatgctgaaa aattcaatgt atgtgataaa gagatagttg aagccaaggt
    15061 gatgggtcaa agacctttaa catttgatga tgtagtaata agagtgagtg acagttttaa
    15121 aacttatatg catatagact atgatgaagc caatgcatgt ggttattcaa aaggaactgt
    15181 tgctaaaata ataaagaaag cataaattta acctactata taaattacct ctagggagga
    15241 tttaatatga attatgataa ccttgtagat ttaataacgg aagaaatcta taaaaaaata
    15301 aatagtaatg aaataaaaat atctaataaa cctaaagctg tgattgtatt tgaacaagat
    15361 aacaataaat ttaacttatt aaaagatgaa tttgaaatac tagaatttga taaaagtata
    15421 agggaatgtg aaatagtgat agtttcaaga ctttgtatga gggggctatc gaatatagca
    15481 cttggaaatt caacaagtga tgaagaaaga tttatactaa aaatgattat gaaaggcaaa
    15541 aaagtatatg tcctagatga aggaattgaa tacaaaaaat ataaggatac tgcaccaaaa
    15601 gcattatata agaaatttat gtcatttgaa gatgaaatat gtaaatttgg tgtagaaatt
    15661 ataaaagact taaatgttat aactaaaaat aagttaaata taaagaaaga actagaaagt
    15721 actagaggtt ctaaaaaaga agatacaaaa gttttagata aagatgtttt aaatttaacc
    15781 agtgtagaaa gtgtaaaaat agataatgaa tttagtttgg atttaaggaa taaaaaactt
    15841 atatcggaag ctgacttaag aaaaccaact ataaatggag ttaaaaacat attagttaat
    15901 aaaaaaagta taataacacc attagctgta gattttatga gaatacacca tctcaaacta
    15961 aagaaactgt aaggaggtaa ttttatggat ataggaaaag ttgtaggtaa tgtttgggct
    16021 acaagaaaag atgaaaaatt atgtggccaa aaacttttag ttgtaaaaat tttaaaaact
    16081 aaagacacat acaaggaagg cttcatggtt gcagccgata atgcaggagc aggaaatgga
    16141 gacttagttc ttatatccaa aggttcctca gctagagagt cgattgaaaa atctcatgtt
    16201 ccagtagatg caactatagt tggagtagtc gattcgtttg aggtgtttga tgaataaaag
    16261 tataggagct atagagttta aaagcattgc taaaggtata gaagtatcca atgaaatgat
    16321 aaagaaatct tcagttgatg tattgtactt aaaaagtatt tgcccaggta aatttctaat
    16381 aatagtaggt ggagaaactt cttatataaa tgaatgtgta gattatggaa taaaacttgg
    16441 agaaggatat attgtagata acttcgtaat aaatgcaatt tcacaagaaa tattagatgg
    16501 atttaaaaat aagtatcaaa agctagatag tatagtctct ataggcgttg ttgaaagtag
    16561 taaagtatgc gctggaataa aaatgcttga taagacttta aaatctggcg acttagtatt
    16621 agtaaaactt caattatctt ttgctatagg tggcaaatta gtatatgtag tagcaggaga
    16681 tttaagtagt ttagaatatg cattaaaaga aagtgaaaat gtagtaagag aaaaagaagt
    16741 aatatataag actgtaatac catcagtaga cagtcaaata ataaaaagtc taataaaata
    16801 atggggggaa ttatgagtat aaacgaaatt ataatctatg taatggtagt cttcatggta
    16861 cttggagcta tagataaatg tataggtaac aaatttggtc ttggagaaca atttgaagaa
    16921 ggtataatgg caatggggtc acttgcagtt gcaatggttg gtgttatatg tttagcacca
    16981 gttcttgctg atgtattaag accaattgta gtaccagtat ttaatatact tggagctgac
    17041 ccagctatgt ttgctggaag tttattagct aatgatatgg gtggtgcgcc acttgcactt
    17101 gaacttgcga aagaccctaa ttctgggtta tttggtggat taatagtagg ggctatgatg
    17161 ggacctacaa tagtatttat aataccagtt gcgcttggaa taattgaaaa agaagaccaa
    17221 aaattcttag caacaggaat acttgcaggt ataataacaa tacctattgg agcattcgtt
    17281 ggtggattag ttgcaggatt cgaggtaatg atggttctta gaaacttagt gccaataata
    17341 atatttgcag tagttatagc acttggatta ttaaaattcg aaaatgctat gataaaagga
    17401 ttcacatact ttggaaaagg tgtagtaata gttataactt taggacttgc tgctgccata
    17461 gttgaagctt taacaggaat agttgtaata cctggaatga aaccaatcag tgaaggtata
    17521 gaaatagttg gagatatagc aatagtttta gcaggagcat tcccattagt gtttgttata
    17581 acaaaggtat ttaataaacc attaatggct ttaggaaaag ttcttggaat gaatgatgta
    17641 tcagcagcag gccttgttgc aagtttagct aactgtatac caatgtttgg tatgatgaaa
    17701 gatatggaca atagaggaaa aataataaat gtagcattct ctgtatcagc agcattcgta
    17761 tttggagacc acttaggatt tacggcagga tttaattcta atatgataac accaatgata
    17821 gttgctaaat tagttggtgg tataacagca gtattgttag ctatggttat cgcaaataag
    17881 acactaagga aggagaatgt ataagatgga tatatcaaat atagataaaa atttaattga
    17941 aacattagta agacaaataa tagaagaaaa aataagtgga actaaagaca cagtagattt
    18001 tgttagaaat aaagacatta gtggaataac ttctataaag ttaccaactg taaaagttag
    18061 tgaatctgat agattagata cagggaatcc aagtgatgta gtttacacaa aagatttatt
    18121 tacattagag gaaagcccaa gattaggttg tggtatgatg gaaatgaaag aaacaacttt
    18181 tgattggact ctaaactatg atgaaataga ttatgtaata gatggaactt tagatataat
    18241 aatagatgga agaaaggttt ctgcttcttc aggtgaatta atattcatac caaaaggaag
    18301 taagatacaa ttttctgtac ctgattatgc tagatttata tatgtaacat atccggcaga
    18361 ttgggcatct caaaattaat aaaaatcaag ttaagttaat aataatcaaa taaataattt
    18421 aaaatacaaa ctcttaaata aacacacata tttaaaaaag ctatgaatta aaattcatag
    18481 ctttttttat aaagaatgtt atattttaga cgtatttttt ctatatttac tgggagacat
    18541 attatatttt ttattaaata atctaataaa atgctctaca tttggataac caatactatt
    18601 ttactgataa tcttttcctt atcactaatc ttacatttta aaatattatt tttactcgta
    18661 atattatcat aagaatttgt ttcttattta aaactaatta gaattataaa gtttattgcc
    18721 atgttattaa taaaataaat ccagttaaat cgagtgctag ttaaagtaat aagaaacttt
    18781 tacaatggtt tcctttatcg attgaaataa aagttaatat ggtataatat ctaaagagag
    18841 aatcttatca attagaatat gcatagaaac aagtggctta aaaggagacg ttatatggaa
    18901 gagatagtaa agaaggaaca agcaagtatg aatgatattt ttttgtatct taaagctaaa
    18961 tcaaactcat tatataaatt tgtaatatta tataatgagt ttatcaataa acctaaagat
    19021 tatggaactg gggaacttat aaacatggtt gaagtacata tgctaacagc gattgaagaa
    19081 aatccaggaa taagtccaac tcagttagct aaaatatgga acagaactaa aggtgcaata
    19141 tctcaaacta tatctaaatt ggagaaaaag ggttatataa ctaaagaaaa gaaagaagga
    19201 aatgcaaaaa ctatattact atatccaacc acatcaggag tagagttaag taaggcacat
    19261 aaactatatg acaccataga tgttacaaga acatttgaat acttaatcaa ggagtgtaca
    19321 ttagaggaga tagatagttt ttataaagta attgatttgt ttattaattt attagaagaa
    19381 tctccgcagg aaaataatga ttctgaaaaa gtgtagctaa gattgaagtt gaataaagag
    19441 aaatagatat tatagaagta gtacttaagt acacattata taaaaatata ataggtatag
    19501 gattaaaaag tttagtggtt taaagtttaa ccactaaact tttttaatgt ctataatcta
    19561 ctattattta atttactaaa aataaaaaaa ctcattatat taagatgaaa atagaaaaat
    19621 aactagaaaa atactataat atatcagctt aatacagaaa aaagttaaaa atattgagaa
    19681 aaataaaaat gaaaacgttt tatattgaca tagagaaaaa gtagtgatac catttaaata
    19741 tagtttagta gctaaactat atttaaatta taataactat tttaggggga tgttatgaat
    19801 ttaaaacaat taagtgaaaa atacgaagaa tatgtaattc aaatgagaag agaatttcat
    19861 aagtatccag aattatcttt taaagaaata agaacaaccc aaagaataag ggaagaatta
    19921 caaaaaatag gggtcccttt tgtatctatt gaaccaaata tcgtaatagc tactatacaa
    19981 ggcaaaaatg aaggtaagac aattgcaatt agaggagata tagatgcgct tccaatgcaa
    20041 gaagattcag atgttgaata caaatcagaa tatgatggaa taatgcattc ttgtggacat
    20101 gatgctcatg gagctatgtt actaggactt gcacatatgc taaatgatat aagaaaagat
    20161 ttaaaaggta gagtattatt agtttttcaa gcagctgaag aagttggtgg cggacacaag
    20221 gagataatac agtactttaa ggaaaatgga ggaccagata gattaatagc tactcatgtt
    20281 tggtccgata ttgaagctgg taaaatttct gttgaaccag gacctcgtat ggctggtggg
    20341 tctggttgga gaatagacat aacaggtaga ggaggtcatg gttctagacc agatcaaagc
    20401 atagatccta taaagccagc ttgtgatata gttttaaaag tatcttctat accagttaat
    20461 catataagtg tactagaaca atgtgttgta catgcatgtg caatacatgg aggaactaca
    20521 ataggaaatg ttattccaaa caatgcagag gtaataggtg gatatagata ttttactgaa
    20581 gaatcaggca aaaaagtgtt tgacattata aagcaaatga gtaatggtgt tggacaagct
    20641 tatggagtgg atgtgaaggt tactcatact ggaggcatag gacctgttat caatgatgaa
    20701 gaagcagtta agatggctaa agaaatagtt tctaatatag atggattgga gttagattct
    20761 tatgagccaa tatgtgcatc agattgttat ggtgaaatat taaaagagta ttctggtttt
    20821 tattgttatc taggggtagg taacaaagaa aaaggaataa tatatgcaca acaccatcct
    20881 aagtacaata tagatgaaga tacattaaag ttaggttgcg aatttatggc taaatatgca
    20941 gttgaatttt taaataaaaa taataactaa aataaatcga attttataaa tgggggggat
    21001 gaaaatgcaa ggtgcattaa aggaaaataa gaaaaaaagt ggtggaggat ttttaggatg
    21061 gattgaaaga gttgggaata aaatgccaca tcctctagca ttatttacat atattacatt
    21121 gatagtagta gcattatctg caatagcaag ctttttaggt ttttcagcaa caagtcctgc
    21181 tacaggaaag ttaataaaag ttatgaactt aataagtgca aaaggtttag tactatttat
    21241 gcaagagttt gttaagaact tccaaaactt ccctgtgctt ggggtagttt tagttatggc
    21301 agttgccaca ggagtttgtg aaaagacagg atttttttca actgccataa aaatgagttt
    21361 atcaaaagtt aaggggaatg cggttgtatt tataatagct tttataggag tatttgctaa
    21421 tcaagctggt gatgctgctt ttgtattagt accaactctt gcaggagcaa tattttatgg
    21481 attaaataga aatccattag ctggtatatt ctgtggattt gcatcagttg gtggtggatt
    21541 tgcaacagct gtaattcctg ggggatggga cgttacgcta acgccaataa ctgtatcagc
    21601 tgctcaaact attcaacctg catttgatat gacattatta gctagttatt atgcactatt
    21661 tgtatctgcg tttatagtag ctacagtatc aacaatagta acggttaaat ttatagaacc
    21721 taaattaggt aagtatactg gtaaaccaga aggtatagat gataatcaag gttttgaggt
    21781 cacaaaagaa caagcaaaag cagcaaagtt agcaggtttt acagttttag cttatgtagc
    21841 gttattaatt gctttgtgta taccagctaa tagtttttta agaagtccag aaggttcttt
    21901 aatatttgga gcaccactta tgtcttgctt acagtttttt atagtaatac tattcttctt
    21961 gccagggatt gtgtatggaa tgagggttgg taaaatcaag actgtaaaag acttaaatga
    22021 tatattatgt caatcaatgg cagcaatggc accgtttatc gtacttgcaa tagttatagg
    22081 tcaattctta acgctattct cagtgtctaa tttagcacag gtactagcta taaaaggtgg
    22141 agcattactc aactcattgc cactaccacc acaagttata atactgctat tcttagtact
    22201 agtagcgttt ataaatatat ttgttataag cggaagtaca aaatggatga tatttggacc
    22261 agtatttgtt ccgatgttaa tgcaattaaa catacatcca gcatttactc agtttgtata
    22321 tagattagga gattctgtaa ctaatcattt atcaccatta aatgcattct ttataatact
    22381 tttagcaaca gtccaaaaat acgataaaaa tgcaggtatg ggaactatat tctcagctat
    22441 gataccttac actataggtt actttgtagt acttgcagtt ttagtagtag tttggatgac
    22501 aataggtata ccagtaggaa taggtggaca agtttggtta taaaacatat ttaataattt
    22561 tgggaggaaa gaaaatggaa acaactgaaa taagatacaa ttttgatgaa gtaatagata
    22621 gaagtggaac aaactgtctt aaatatgatc ttaagagtca atttatgcca gaatctccag
    22681 aagatgcact accactatgg gtagcagaca tggattttgc atgtgctcct gagataatta
    22741 atgcaatgca tgaaagaata gacagaaaaa tatttggata tagctctcaa caaactgatg
    22801 attattatga agctgtatgt ggatggttta agagaagatt tgattggaaa atagaaaaag
    22861 agaatatatt ctttagccca ggggttgtgc ctgcgttagg atttttcata caaatattaa
    22921 cacaaaaggg tgatggtatc ataatagaaa agcctgtata ctatcctttt gaagataaaa
    22981 taactaataa tgaaagaaaa gtagttaata gtcctttaaa gtttgaagat ggtcattatt
    23041 ctatggatta tgatggattt gaagaattag ctaaagatga aaataataag gtgtttatat
    23101 tatgtagtcc tcataatcca gttggtagag tatggaaaaa agaagaactt agaaaaatag
    23161 tagacatatg taagaaatat gatttgtgga taatatctga tgaaatacat tgtgatatta
    23221 ttcgtaaaag tgaaaagcat ataccattag aagtagcatg tcctgattat aaagatagaa
    23281 taataacttg tacagctcca agtaagagtt ttaacttagc tggtatgcaa ttatcaaata
    23341 tagtaatcaa taataaggaa ttacaagaaa aatggatgct tgaaacagat tctaaattag
    23401 ctatattcct acctaatcca ttcgcaatag ttgctactaa agttgcttat aatgattgtg
    23461 aagattggat gaatcaagta aatgagtata tagatggtaa tattaaatat gtagaagatt
    23521 ttgttaatga gtatatgcct aaagtaaaga taataaaatc tcaaggaact tacttaattt
    23581 ggttagactt tagagggtat ggattagatg caaaacagct tgaatatgta atgcttaaca
    23641 aggcgaaggt attgctagat gaaggataca tattcggaga agaaggagca ggatttgaaa
    23701 gaataaatgt tgcttcgcct agaacaatta tagaagagtg tatgaataga ataaaatgtg
    23761 catttgaagg attataatag taatatctaa ataatatatt tgtgtatttt aaaaaaagac
    23821 tatgttaaaa aaatatttca aagcatagtc ttttaaagtt ttatgatgag tttaaaaaat
    23881 ttcaaaacgt tactattatt gataatcttc acttatatgt ttatattaga aataattcca
    23941 caatatgctc attttctaaa gttgcacttt tatacacctt gcttcaccaa tatcatgtgt
    24001 aaccaaaata gtagacttat tctcattctt aataatacta taaacatcat cacaaactaa
    24061 taatctagtc tgataatcaa gagctgaaaa aggttcatct aaaagtagaa tatctggatt
    24121 tacagaaagt gttctaataa gcgcaactct ttgtctcata cctccagata attcttttgg
    24181 atacatactt ctaaaatccc ataatccata agtctttaaa aggctttcaa ctctatctaa
    24241 agatttttta tcttttttgc cttgaatttc caatccaata gttatattat ccattatatt
    24301 tctccattca agcaaattat ctttttgaaa catatatcca atatttccag taatctttat
    24361 atcaccacta gtcggtttta aaagattagt aagaatattt aatattgtag atttaccact
    24421 tccagaagga ccaagcaaag ttaatatttc tccttctttc aagttaaaat tgacatcctt
    24481 taatacttca agctcgcctt ccttagtgta gaagttcata tttatatttt taacttctac
    24541 tatgttgtca ttcattatgc caccccctat aacttttagc tcgtatatat tatgttatta
    24601 acaatatgac ctatttgtac catttttcat aaatataata taaatatttt gtttatttac
    24661 gtattttctt agtaacttgt acgtataaat tataataaat aaattataag gtaggggata
    24721 aatttgagta acataataac gaatttaata gagtcagtat ttactaatcc aataactata
    24781 ttgtttttac ttgcaataac tgtgtattct gtttttaaaa taatagaaaa taacagagta
    24841 tattcatttg taagttcaaa attagacgaa atcaatagag attataagac aagtgagttt
    24901 tataaaaagg taagggatga ctactataca tatagtaaag aaaatcctta tgcagacgta
    24961 aatataactt catttataga ggaagttgta tctgatttaa agcataataa tttgccattg
    25021 ttagaaaaaa ttagaagtat aaaaaattca tcttcaataa gtatattact tggggtttta
    25081 ggtacgtttg ttggtctttc tacaatgctt ttatgtgttg atactaaaga cattataaat
    25141 tcacttcctt caactattag tagtatgcaa actgcattta caactagtat atttggagtt
    25201 gtattttct
//
LOCUS       DAEPOZ010000029        24677 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.029, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000029 DAEPOZ010000000
VERSION     DAEPOZ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24677
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.029"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            67..417
                     /locus_tag="KR440_000984"
     CDS             67..417
                     /locus_tag="KR440_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBH0698044.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            436..2079
                     /locus_tag="KR440_000985"
     CDS             436..2079
                     /locus_tag="KR440_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBH0698045.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            2160..4217
                     /gene="recG"
                     /locus_tag="KR440_000986"
     CDS             2160..4217
                     /gene="recG"
                     /locus_tag="KR440_000986"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBH0698046.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIRNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMERFKNKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            4276..4830
                     /gene="rsmD"
                     /locus_tag="KR440_000987"
     CDS             4276..4830
                     /gene="rsmD"
                     /locus_tag="KR440_000987"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBH0698047.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNVKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            4832..5329
                     /gene="coaD"
                     /locus_tag="KR440_000988"
     CDS             4832..5329
                     /gene="coaD"
                     /locus_tag="KR440_000988"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBH0698048.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            5333..5857
                     /locus_tag="KR440_000989"
     CDS             5333..5857
                     /locus_tag="KR440_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698049.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            6201..8120
                     /locus_tag="KR440_000990"
     CDS             6201..8120
                     /locus_tag="KR440_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH0698050.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQERFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENVL
                     EINSISKEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKSVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSILELINKQN"
     gene            8133..8420
                     /locus_tag="KR440_000991"
     CDS             8133..8420
                     /locus_tag="KR440_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0698051.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            8553..9812
                     /locus_tag="KR440_000992"
     CDS             8553..9812
                     /locus_tag="KR440_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBH0698052.1"
                     /translation="MLDFISNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAF
                     GIIIMETGTGMLVSSIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAI
                     HLMIARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPW
                     LLRKYVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREIS
                     ISGGMAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQI
                     MPAFQGISEKVVPNALPAFDCPILFNYKPNAVLIGFLVAMLTSTAMILICNYFNVFNL
                     LLIPLVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNW
                     ILIFGGNDLSLFGIISKFIGSLFGGIL"
     gene            9815..10555
                     /locus_tag="KR440_000993"
     CDS             9815..10555
                     /locus_tag="KR440_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBH0698053.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQILVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            10878..12188
                     /locus_tag="KR440_000994"
     CDS             10878..12188
                     /locus_tag="KR440_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBH0698054.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAANFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            12286..13203
                     /locus_tag="KR440_000995"
     CDS             12286..13203
                     /locus_tag="KR440_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBH0698055.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVKRDNSDF"
     gene            13219..14028
                     /locus_tag="KR440_000996"
     CDS             13219..14028
                     /locus_tag="KR440_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBH0698056.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            14085..15833
                     /locus_tag="KR440_000997"
     CDS             14085..15833
                     /locus_tag="KR440_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HBH0698057.1"
                     /translation="MEISYVLKDCTFKHNKYSVIEAETWKEKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSTISWKGLENRVRLALNVPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLNIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDKSKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYISIADKY
                     KMSKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDENDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVGTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIENLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKGREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     GYDKVKKYVLELLDRV"
     gene            16096..17118
                     /locus_tag="KR440_000998"
     CDS             16096..17118
                     /locus_tag="KR440_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HBH0698058.1"
                     /translation="MFGGINIKRIGIIIMFFLSIAFIGFYLAIRGNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEEFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLSSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNVPKDSNIVV
                     SFHYYEPNDFAFQGNIYHNGFEHLSNITWEGTNEQMDYLKKRFDIVENWANKNKVKIF
                     LGEFGVTKEAPEASRRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILS
                     ALIEKR"
     gene            17123..19429
                     /locus_tag="KR440_000999"
     CDS             17123..19429
                     /locus_tag="KR440_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBH0698059.1"
                     /translation="MKKNSKKILITLTIITNIVYILWRIFYTVPKEEGMFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPVISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMRILAEKMGVNYITRTEREGAKAGNLNNAMQHTNS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKNKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKIRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKNIYPKEIALEINENIVLECDLCEGKIDERGLLYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKTIPVSNPSEKKEKSIDEFEPMEYI"
     gene            19401..20516
                     /locus_tag="KR440_001000"
     CDS             19401..20516
                     /locus_tag="KR440_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698060.1"
                     /translation="MSLNPWNIFRRDEMKTLKVFFCSAKYLILVFIFFINTTFISSAT
                     SDKILDLSFKKIETDLSSRITYEDTGVKIETDSSKSDKERYLYIYQNIKENWSMYNNF
                     YIEIQNKNKSSQKINLSIQSKNMLEFRLKEGSEIFLEDKNIIYSDKIKEGCIEVPGEF
                     EGKIYVNFNSLINEKSNVVLDSSMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKL
                     SFLHNIKITGNEEVQIPVLGQSISQYEVLGLKRDSKIKYSLMGKQDNVSISQNGKLTL
                     SNKAEPGQIILQVNVDDKFKIGRKIALTESWSINKKDKDGVPYTLVSPEQSPTVQYMK
                     KINFMNNIITFVRILFVSLVIICFGIYLYWKKCSKTK"
     gene            20534..21952
                     /locus_tag="KR440_001001"
     CDS             20534..21952
                     /locus_tag="KR440_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HBH0698061.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCVYNIGVLFIFKYLAFAL
                     RNISELINTELTIPSIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTMQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMLGG
                     NGVIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            21967..23175
                     /locus_tag="KR440_001002"
     CDS             21967..23175
                     /locus_tag="KR440_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698062.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNICYSGKDMIQEIKNSVAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSEEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKENYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCK"
     gene            complement(23256..24644)
                     /locus_tag="KR440_001003"
     CDS             complement(23256..24644)
                     /locus_tag="KR440_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBH0698063.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVTWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IDFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFTIHLIFITLNGLNPITYIRNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSLYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFGKKNIS"
BASE COUNT         9628 a         2654 c         4421 g         7974 t
ORIGIN      
        1 aagtaaaaat agtaaaaaat aaattaagaa atgtttttaa taaaaataag cttaaggggg
       61 tcaaccatga gtgcaaaggt aacgaatcaa tatggaaata tagaaataga taatcaagta
      121 atagctcaag tcgtatatag agctgcaatg gaaagttatg gcttagtagg tttagcatat
      181 aagtctaaag gaatagtaga gctattaaaa ggcgagaacg caactaaagg tgtaagagtt
      241 gaggaagtag aagaagatgc catagcaatc gaactttatg ttataataca atatggtaca
      301 aatatatcta ctgttgccaa taatataata gatagagtaa agtatgtagt tgaaaaaatg
      361 actggcgtaa gagttactag aattgatatt aatgtacaag gaataagagt taagtaattt
      421 cataggagga aacaaatgat tcaatatata gatggtaaaa ggttaaggga gatgtttatt
      481 tcaggtgcaa acaatctcca aaacaataaa gagttagttg acaagttaaa tgtattccct
      541 gtaccagatg gagatacagg aactaatatg tcattaacaa tatcttatgc cttaaaagaa
      601 ttagcaaaag ttgaaaatga taatatatca gatatcggaa aagcattatc taaaggttct
      661 ttaatgggag ctagaggaaa ctcaggagtt atactttcac aaataatcag aggtatagct
      721 aaatctatag aaggaaaaag taaattatca acagaagatt tagcaaaagc ttttaaaaat
      781 ggttcagaca cagcatataa agctgtaata aaaccaattg aaggaactat acttactgta
      841 gttagagaaa gtggagagtt tgcaataaaa actgcgaaaa aagagaaaga tgtagtaaag
      901 tttttaagta tgcttgtgaa ggaatcaaat tcttcattag aaagaactcc agaccttcta
      961 aagaatctaa aagaagctgg tgtagttgac tctggtggaa agggtcttgt acttatatat
     1021 gaaggaatgt tagcttctat aaaaggcgat aacatagaaa taaaaaatgt tgacctagat
     1081 actaatattt caacaagtat ggattttgca aagagtacta ctagcacaga tgatataaaa
     1141 tattgctatt gtacagaatt tattttagaa agttctaagg tagaagatac taaaataaga
     1201 gacataatga tggcttatgg tgatagtcta gcagtagttg gagatgatgg agtcataaag
     1261 gtacatgtac acactaatga cccaggaaat gtacttcaag aagctctaaa gtacggtcaa
     1321 ttattaacta taaaaataga aaatatgaag ttacaacatg aaaatacatt actggatgta
     1381 gaagaaaaga aagaaaatga tagtgaacct ttagaagaag agaaagagtt tgggtttata
     1441 gctacttcta tgggtgaagg tttggcaaat atatttaaag atttcggagt tgaccatata
     1501 atagaaggtg gtcaaactat gaatccaagt actgaagatt ttatgaatgc tataaagcat
     1561 ataaatgcta aaaatatatt tattttccca aataacagca atataataat ggctgctaat
     1621 caggcaaaag aattgagtga taaaaatata atagttatac ctacaaaaaa tacgcctcaa
     1681 ggatttgctg cattagtaac ttttaatgga gaattaagcg aagatgaaaa taaagaagct
     1741 atgatgaatg ctttaaactc agttaaatca ggtcaagtaa cttttgctgt tagggataca
     1801 gtgatgaatg aaattgatgt taaagaaggt aatataattg gaattgcaga aggaaattta
     1861 ttatcagcag gagattatgt ggatgaagta acatcaaacc ttattgaaaa gttggttgat
     1921 gaagatactg ctataataac attattcttt ggtgaagatg ttacagaatc tcaagcaaat
     1981 gaactacgta cttctttaga agaaaagttt gaagatgtag atgtagagtt atattatgga
     2041 ggacaacctc tttattatta cttaatttca gttgaataat gattataaga acctgtatga
     2101 ccaagtagct tttataaaat tggttgtaca ggatttttgt gtagtaggtg atatatttgt
     2161 tggatttata taaagatgtt caatatgtta agggaatagg acctaaaaaa gcagataaat
     2221 taaataaact gggtatattt acactaaaag acctattgta ttattttcca agacaatttg
     2281 aagatagaaa taatctaaag aaaatagcac aattagaaga tggtgagaaa gttacgataa
     2341 aagcagtaat atcaagtata aatacattta gcccaaaaga aggtatgact ttaacaaaga
     2401 ttgatgtaaa agatgagaca ggctcggcta aattagtgtt ttttaataaa agttatataa
     2461 aaaatacttt tcgtccagga gattcaatat tagtttttgg gaaggttaag aaaaaattta
     2521 ataacttaga acttacatca tgtgaattag aatatcttac taattcacct aagaatacat
     2581 gtagatttat gcctgtgtac cagcttacat acggtgttac aaataaagaa attatgagta
     2641 taattagaac agtacttgaa gataaagaat taattataca agaatatatg ccacaaagaa
     2701 ttatagaaaa atataggcta tgtagtatag attttgcagt tagaaacata cattcaccaa
     2761 gtagtaagga atcattaaaa atagctttat atagaattgt atttgaagaa ttacttatac
     2821 tacaactagg gttgtttgta tttaaaagtg gcagaaataa agaagatgga ataaagtttg
     2881 aaacaagtaa agacttaaaa aaaataataa gtgctttacc atttaaactg actaaagctc
     2941 aaaacagggc attagatgaa attatacaag atatgaattt agagaaaata atgaatagat
     3001 tggtacaagg tgatgtaggg agtggtaaaa cagtagtagc tctcttagca cttgcaaatt
     3061 gtgttttaaa tggttatcag ggcgctttga tggcacctac agagatatta gcagggcaac
     3121 attacatttc acttactgag tctctaaaag attttggaat aaatgtaggt ctattaatag
     3181 gtagtcttac aaaaaaacaa aaggatacag tattggaaca aataaggaat aatgagattg
     3241 atatattaat aggtacacat gcactaattg aagataaggt tgaatttaat aatattggtt
     3301 tagttataac agatgaacag catagatttg gtgttatgca aaggagcaaa ctttcattga
     3361 aaggagcaaa tcctgatata ttagtaatga cagcaacacc aataccaaga actttagcac
     3421 ttatactcta tggagattta gatatttcta ttatagatga acttcctcca ggaagacagc
     3481 caatagaaac aattgctatt gaaaaaagta agcgagatag agcatataat aaccttgtaa
     3541 gaagagaggt tgaatcagga agacaagtgt acattgtatg tcctttggta gaagaaagtg
     3601 aagctataga agctaaatca gcagttgaac ttgtagagga attaagagct gaatatttcc
     3661 acgatttaag gcttggattg cttcatggta aaatgaaatc gagtgaaaaa gacgaagtaa
     3721 tggaacgttt taaaaataaa gaaatagata ttttagtttc aacaacagtt atagaagttg
     3781 gtgtcaatgt cccaaatgca acattaatga taatagaaaa tgcagaaaga tttggacttg
     3841 cacaattaca tcagcttaga gggcgagttg gaagaggtag tcataaatcg tattgtgtac
     3901 taatttatga ttctaaaaca gatgtatgta gacaaagaat ggctataatg gaagagacca
     3961 atgatggttt taagatttca gagaaggatt tagaaataag aggacctgga gaattttttg
     4021 ggacaaggca acatggtctt ccagaactta aagtagcaaa cttatttaaa catataaaaa
     4081 tattgaagtt agcacaacaa gaagctcgat atatattggg tgaagataat aatctacaat
     4141 tgaaagaaaa catggcactc aaaaaagaaa ttatagataa atttaaagat acattgaaag
     4201 aaatttcatt aaattaattt taaattatgt taaaatatta ctaatgtaat aatgtgctga
     4261 agggagattg tcatattgag agtaatttca ggaaaagcga gaggattaaa actaaatact
     4321 ccaaaaaatg aagatgtaag gccaactaca gatagagtaa aagagtctct atttaatatg
     4381 ataaattcat atataatgga aagtgaagtt ctggatttat ttgctgggac tggttcttta
     4441 ggaatagagt gcttatctag gggtgccaag tcatgtactt ttgtagatat cagtaaggaa
     4501 agcatagata ttgttaagtc caatgtaaaa aaggcaagag ttgaaagtga aagtattatt
     4561 ttaaaccttg attttaagac tgcaatagat aaacttaaat tacaaaatag taaatttgac
     4621 ataattttta tggaccctcc gtattataaa aatatgttta tagaagctat tgaaaaaatt
     4681 gataattcta atctattgaa cgaagatgga ataatagttg tagaacatga tacgaatgat
     4741 ttatttccag ataagatatg taaattggag aaaactagag ataagaaata tggaaatact
     4801 acattaactt tttataagat ggaggcataa tatggaaaat aaacctagaa aagctatatt
     4861 tgcaggaagt tttgacccaa ttactaatgg acatttagat attatttgca gggcttcgaa
     4921 gttatttgac gaattacaaa taggagtatt aaataatcct aataaaaagg gtctatttag
     4981 ctttgatgaa agagtaaaac ttatagaaaa aagtacaagc catttaaata atataaaagt
     5041 tgtgactttt gatggtttat taataaatta ctgtcaggaa aatgggattg gagcattagt
     5101 tagaggtgtg agaagtggtg ctgatgttga ttatgaactt cagatggcac atatgaatag
     5161 agagcttaat ccagatatag agactataat tttaccaagt tgtacaaaat attcatttat
     5221 aagttcatct ttaataaaag aggttttgct ctttgatgct gatataaaaa atttggttcc
     5281 aaagattgtt ttggaagaat taaagaaaaa aactagtggg ggtaattgat atatgaagat
     5341 agatttagaa atgattgaac tatttgagca gttagaggag atatttagaa gtgcttctac
     5401 aatccctttc tcacataaat gcacagtgga taaagatgag gttttagcaa taattaatga
     5461 tataagaact ttaataccag aagaagtaac acaggcagta tggattaata aagaaagaaa
     5521 taaaataata agtcaagcta agcaagatgc tacaaatata gttgaacaag cgaaaaaaga
     5581 agctgagaga atacaacaag aatatcaaga taatatagag gaattaaaga aaaattctga
     5641 agatgtagta aaggcatatg tagaatccag cgaacctgtt gttcaagcag accaaagagc
     5701 aaaagatata gttgatagag ctgagagaat agccaatgag ataagaattg ggtcaattga
     5761 atatgcagag gatgtgcttt ctagtgttga gtataattta agagaaatat tagaagaagt
     5821 aaaaagagat aaagctgaac ttagacctaa aaaataaatt ttaaataaat aaaaatggag
     5881 ctatcacaca aaaaattcca attgatgtgt gatagctctc ttttaatata gcaatttttt
     5941 gttatataat tgtgcttttt agattactta tatcttattt tgatattata atgtagctta
     6001 atcttttatt tgttgaaatc aacttattta tttgtataaa taatataggt tattttcaaa
     6061 tttttttaat aattttacca ctgtcttctt ttagattagg gttagactgt gcaaaattat
     6121 aaaataatac taaatcatgt gcaattattt ggtatggcac tacatgtaga aaatatagat
     6181 aataaaatta aggaggtagt atgaaggaaa gggctaaaaa aatattagat tttattatta
     6241 aaaataacaa tgtaacatca caagaactac aagaaaggtt taatgtttca aagagaacta
     6301 tttattacga tattctagct ataaatgaac aactaggaaa aagtggtaat ataaaaaatg
     6361 taaaacacaa atttatattt gaaggtaatt tatgtgatgc aaggaaaata atttcaactg
     6421 aagaagataa atttttagat agtgattaca gaaaaacttt tatattaaac aagatattac
     6481 ttggcgaaaa aatatcaata gaaaaactaa caaatgaaat gttactttca aaaaacacag
     6541 tagtacaaac aataactgat gtaaaaaaat accttcaaac aatggggtta agattagagt
     6601 ataagggaaa gtacaaaatt ataggtgatg aatacgttat tagagagctg tttctgataa
     6661 ttgttcaaga aaatgtactg gaaattaatt ctataagtaa ggaagtaagt agtttcgata
     6721 caaaaggaca tataaaactt actgattatt ctttattaaa tctaactaag tttgtggaat
     6781 ttctaaataa aaggataaga gatgggaaaa cattatactc atataaatat ctaaatgaag
     6841 ctaaaaaaat tagttacttt agtaattgta aagaactttt atgtgaagaa gccaatgaaa
     6901 atgaacaagc ctatatatgc acttacataa gttcacttcc tagcttaaat agtgaagtta
     6961 aggaagatgt tgttgaagaa tatgtagata aacttataga taagtttgaa gtaaatacag
     7021 caataaaatt agaaagtaag catgagttta agaaaaatat tttaaggcac ctccactctt
     7081 cttacaacag aatacgattt aagtttccta ttagaaatcc aatgttagat gagactaagt
     7141 ataaacatga gtcactttat aaaattataa aatcaattat agaaaatgaa gaagagtttc
     7201 ctgtatttga ggggataaga gaagaagaga taggatttat agcagcgtat tttggagggt
     7261 atttaagagg gtctagggat aatggcctaa gaagaaataa agtcttacta gtttgtccta
     7321 atggtcttat ggtatcaaag agtttggaaa tacaactata taaatacata ccaaccatag
     7381 aaatagtagg tatagtgtcc attaagcaac taaaagaagt aaatgtatac tacgattaca
     7441 taattacaac tatagatata caaaatgtaa acaatgtaat tgttgtaaat cctcttttaa
     7501 catcatcaga tgttcaatta cttatgaaca aactaataag tgtaaaagaa aacgagaagt
     7561 atttcaatct tgaattaata atacaagcta taagaaaaaa tggagttatc aataatgaag
     7621 aagcactaaa agctgattta ttaaacataa tacacaagat agatgaagga gagatgtatc
     7681 aaccaatgtt aaaggaatta attaatgcag aaagggtcaa tataataaaa agtgttagag
     7741 attggaaaga agcaataaaa atagcctcaa agccattgtt agaagataac tctatagaag
     7801 agttatatat agaaaatatg ataaagtcag ttgaaaaata tggaccatat attgttttag
     7861 ctgatagatt tgctctacca catgcaagtt ctaaagaagg agtaaacaag cttgctatgt
     7921 ctttactaat agtagaagat gaagttgatt tattaggaaa accagtaaat atatttatgg
     7981 tattagctgc tgtagataac actactcaca ttagagcatt agcatcacta tcagagatga
     8041 tgtacgagga agaaaatgta aaactaataa ttaatggtga taaaagttca atactagaac
     8101 ttataaataa gcaaaattag gaggaatgaa aaatgaagat attagcagta tgtggttttg
     8161 gtgtaggttc atcaatggta ttaaaaatga caatacaaaa ggctctaaag cagttaggta
     8221 tagaatgtga agtagaaaat acagatataa attcagctag aggtgctgat tgtgactgta
     8281 tattcacaag tcaagaattg ttaaatgaat taaaaggaac ttgtaatgta gatgtatatc
     8341 cagttaaaaa gtacatggat ttagcagaag tcacagaagt tgttcaaact tttttaagtg
     8401 ataaaaatga taagaaatag tataagtttt aaaaagtaaa tttaagttaa tcagaataaa
     8461 ataaaaactt actaaattca aaaatagtaa gaaagagagg agggatttag tggtagaaaa
     8521 cttaaacagt tgacctatca ctaaaaaata taatgcttga ttttatttca aataatattt
     8581 tacgtaatcc atctatgtta attggattaa tagcagcatt tggtcttgta ctacagaaaa
     8641 agaaagcatc tgatgttata aaggggtcac tacttgcagc tttcggaatt ataattatgg
     8701 agactggaac aggtatgtta gttagttcaa tagcaccaat taatggagct ttccaaagta
     8761 taagtggtgg tgcagtaaca aaaggtttaa gtgatgttac ttttactgca agttatggtg
     8821 gaattatagg tttagcaatg tttgcgggtt tagctattca cctaatgata gcaagattta
     8881 ctcctattaa gacaatcttc ttaacaggtc acatgcttta ttggttccca ttcatatttg
     8941 tagcttcagg agttgaagct ggattaaatg gtggagtatt aatagctttt gcagcagtat
     9001 tatcagcact ctattggtca ataatgcctt ggttattaag aaaatatgtg tttgatgtta
     9061 taggagatga ttcttttaca ttaggtcatc catctggttg tttagcactt atatcaggat
     9121 ttgttgctaa attagtagga aataaagaaa aatctacaga agatataaac atacctcaaa
     9181 gtttatcatt ttttagagaa atatctataa gtggtggaat ggcagtgttt ttaatgttta
     9241 tagttgttgg gatagtaata ccaggtgcgt ttaaagatag taatgagttt attggaacgt
     9301 atgcaataaa gcaaggtctt tcttttggag caggtcttat aataatgctt catggggtaa
     9361 gaatgctaat aaatcaaata atgccagcat ttcaaggtat atctgaaaaa gttgtgccaa
     9421 atgcactacc tgcttttgac tgtccaatat tatttaatta taaaccaaat gctgtattaa
     9481 taggattctt agttgctatg ctaacatcta cagctatgat tttaatatgt aactacttta
     9541 atgtatttaa tttgctactt ataccacttg ttataacttc attctttgag tgtggagcag
     9601 cagcagtaat aggagaatca caaggtggta tacgtggagc tataataggt acagctatag
     9661 ctgcaatatt tatggtagtt ttagtaggaa tatctgctgt aatttactca ggaactattc
     9721 aaaattggat actaatattt ggaggtaacg acctttcatt atttggtata attagtaagt
     9781 ttataggttc actatttgga ggaatattat agatatgaaa tttaaataca tagatgagat
     9841 aagaagatta attgatataa tagaaaacga agaaaaagtc aatatggaaa aagcagtaca
     9901 aatccttgtt gaagcaatag aaagtaaaaa tgctatattt tcatttggag cttcacatgc
     9961 aggaatatta acagaagaat tattctatag agcaggtggc tgtgttctta taaatccagt
    10021 atttgcaaaa tctctaatgt tagatacagc gcctataact cacacaagta agatggaaag
    10081 attagaagga tatggtacag ctctagctga taaaactcca attaaaaaag gagatgtagt
    10141 tatagttcat tcagtatctg gtagaaatcc agtaagtata gagtttgcta tgaaagctaa
    10201 ggaaaataag gcaatagtaa tatgtataac taatttaagt tactcaaaaa gtgtatcttc
    10261 aagacattct tcaggaaaaa aactttatga agtatgcgat ttagtgatag ataatcatgg
    10321 tcaaaaggga gatgcctgtg tagagataga tggattaaac caaaaagtta gtccaacttc
    10381 tactgtaata gcaactactg tgatgaactc tattatagct caagctactc aggaattagt
    10441 taataaaggg ctaaagaatc ctccgatttt ctatagtgcc aatattgatg gtggagatga
    10501 gttaaataag aagatttttg atgaatataa aagtgtaatt cattatgaat attaaggtta
    10561 aaaaaatatg ttaatgaact ttgaagttta tcaacaaaga atattaataa tatataataa
    10621 atgctatcat ttaaaagctg taggtaaaaa tctacagctt tttattattg tagattaaaa
    10681 agatgcgtaa ttaaatttta aattggagaa aataaattgg aaggtatcaa ttataaatat
    10741 tagaaacgtt gtcatagaaa aaaactatat taaaaaaaag tacatagaaa aaactgatta
    10801 gacaataaat acatgctttg ataatatttt aaatggagtg aaaatgtgta gaattataat
    10861 gaaaaaagaa aataaaaatg aaaaaagaaa ataaaatgga gggaataaaa gtgggtatgt
    10921 taaaaaaatt atgttcacta gctatggtag caattatgac tacaacactt ataacagggt
    10981 gttcaagtgg aaatggaaaa gattcaaaaa aggatggcga aaaagaaaaa ctagttcttt
    11041 ggatgccacc agtagaaaat aatatgaagg aagtttggga ccctatttta gataaatttg
    11101 aggaaaaaaa taactgtgaa gtagatttac agataattcc ttgggataat tattctgaaa
    11161 aatttgcaac agctataagt gctggcgaag gtccagatgt aggatatatg tatgcagaaa
    11221 tgtatccaca gtttatagaa agtggagcag tagaagattt gactaactat gcaactaaag
    11281 aggataaaga gcaatctatt tatataaaaa catctgaaat gatgggtgga atgtatggta
    11341 tgccattcca agcagcaaat ccaggggtat tatactataa caaggatatt ttagagaaat
    11401 taggagagaa acctcctaag acatgggaag attttaaaag aatttgtaaa aaagcaacac
    11461 aagatactga tggtgatgga aagatagacc aatggggatt agctcaggga tggggagcta
    11521 aaacttttgg taacttaaac tggaactggt atccttattt atggcaagct ggtggagata
    11581 tttttaatga tgatttaaaa tcggttaaat ttaatgataa gagtggttta gaagcagcta
    11641 atttcttaaa agaattacaa gcatatgtgc ctgaagattc tttatctaaa gatagtaatg
    11701 aaatgataga aagcgtattt ggaccaggga aagcagcatt tacaataatg ttatcttctg
    11761 ctgcaacaag tgtatttgat gaatcattcc cggatttaaa ttgggggttt gtaactggac
    11821 tagaagataa gaaagcagca acttttggag cagtagacca tttatcttta atgtcatctg
    11881 ctaaaaataa agaacttgca tacaagttaa tacaacatat gttaagtgtt gaatcaatga
    11941 cagaatttca taaagcaata ccaagagcac caataacaaa aggtgaacca tatcaaggtg
    12001 atgaaagatt caaagaaatg gtggaaaatg acaaaaatgt atatagacca ttagtagtag
    12061 gacctcatgg agttgaaata tatgaatatt tatggaaaga acttcaaact atgatttcag
    12121 gagataaaac tccaaaacaa gcattagatg atgctgcaaa atattcaaat gatttattag
    12181 cacaataaaa gtttaaagct gtaatatgtt gtaaaggact ttacagatta aaattagcat
    12241 tgtatttata aatacaatgc taatatttat attggagagg ataaaatgga aaacttagtt
    12301 agagagtttg aagaaaattc aaaaagaaaa aaaacaaaat taaatattaa accatatatg
    12361 tatatattgc cattgggaat aattttagtg gcgttttatg tgataccaat aataatgtca
    12421 atatacttta gttttacaaa gtataatatt attagtccag ccacttttat aggtttagaa
    12481 aattataaaa aattatttac agatgaaaca ttaaaagtat ctattataaa tactattaaa
    12541 tttactgttg ttgtagtacc ttgccaaact atattatcat tgattttggc agtttggatt
    12601 acaggaaaag gaaatagtaa aatagcttca tttgctaaag gtgctatatt tataccagta
    12661 ttatcatcta tggttttgat aggtatggta tggagggctt tattgaatgg agaaggttct
    12721 attatatatc aggtattagg tgcctttgga atagaatcat ctaaactttt aggagatagt
    12781 aagacagcat tacctacact tatgtttata tctatgtgga aaaatatagg gtatttcatg
    12841 gtaatttata tttcagctat aatgaattta ccaaaacact gctatgaagt agcgaaggtt
    12901 gatggggcta caaaacttca agaatttata aaaataactg tacctttatt gaaaccaact
    12961 acaataatgg ttgtattttt agggtcgata tggtcattac aagtatttga cttaatttat
    13021 actgtgacag gtggaggtcc aggtatatct actatgagta tagttatgca tgcatttaac
    13081 ttaaacttta aaaactttaa ttctggatat gcaatgactg tagcaaacgt attatttttg
    13141 ttaatagcag tagtttcgat tttacaaaat aagcttgtaa aacgagacaa ttcagatttt
    13201 tagaaaggta ggtataatat gaaaacagca agaagattat taagtgatgg tttactttta
    13261 tttatagcgt gtgcaagttt ggtacctttt atatacatgt tgataatatc attgaagata
    13321 acatataact catatagttt ggatatatct ttttcaacag taactcttca aaactatata
    13381 gatatcttta ctaaaaaagg atttgcacaa tattttttta atacagcaat agtatctttt
    13441 tcaggtgtat tattaaattt agtatttagt acattagcag gatattcttt tgcgaagatg
    13501 gattttaaag gtagtgataa gttattttta tttatgataa tgactttaat tataccttcc
    13561 caagttacaa tgataccact atatataata atgaaacact taggatggat aaatagttat
    13621 cttgcactta ttatgccaat accaactgca tttggcgtat ttataatgag acaagctatt
    13681 ttaggagtac caaaagagtt attagaatca gcaaagatag atggttgctc ggattttaga
    13741 attttaatac aaatagtatt gcctttaatc aaaccagcat taattacact agcaatattt
    13801 acttttatgg gagcatggaa cgagtttatg tggcctctta tagctacaac taaagatgca
    13861 atgagaacat taacagttgg attatctact cttaagacat tccagataac taattatgga
    13921 cagatgatgg caggggctac aataacattt ttacctccat ttattttcta tttgatttta
    13981 caaagtaaat ttgttgaagg tgtgtctttg tctggtataa aaggttaaat agtctaattt
    14041 aataacataa aagaaactta tataaatatg gaggaattta aactatggaa atttcatatg
    14101 tactgaaaga ttgcacattc aaacacaata aatactcagt tatagaagct gaaacttgga
    14161 aagagaagga tttggatatt gtagtagcta aaggtgaaaa atttgcattt caaattatgc
    14221 taaaagcaac agaagaattt aattgtacaa ttgataaaag cagtactata tcatggaaag
    14281 gactggaaaa tagagtaaga ttagcattaa atgtgcctaa tagtttagaa aataatttta
    14341 gtataaatat attaggatat gttcaagatg atacaaaggc atttgtgaat gatgctattt
    14401 taagggataa agatgtgtta gtagagcaat atttgcctca aacattttgg attgaaggtc
    14461 aagtaccaga agattttgaa gaaaataacc ttaatatagg tattgatata tttaaaagct
    14521 ttggatatga agatgaagaa aaagtttgta caattgatgt tcctgtaaaa gtaagaaatg
    14581 tcgtgctaaa gcctttagat aaaagcaagt tctttttaga tttatggcaa catccaagtt
    14641 gtttagctag aatgtataaa gttgagttat ggtctgatat acattttgaa atagtcgata
    14701 actatttaaa agaattagca tctttaggag aaaaagttgc aacagttata gtttcagatt
    14761 atccatgggc agggcaaagt tgttataaag tttataaaaa tccatcaaac ctatatgaat
    14821 ataacatggt aagtgtatca aaaggattgg atggaaagat taaatgtaac tttgagagta
    14881 tggataggta tataagcata gctgataagt ataaaatgtc taaggagata gatttatttg
    14941 gtctattagg taattggtgt gctggtgagt ttggaaatcc tgtagaaggt tataaggacc
    15001 ctataagagt aagatatttt gatgaaaatg ataaagtatt taaatttata aacaatacaa
    15061 atgatttaaa agaatatata ggattagtat taaatcattt aatagagtgt ggattatggg
    15121 atagagtaag gattatagct gatgaaccaa ataatccaga agtagtcaaa gagtgtatag
    15181 aatttataaa ttctactgta ggtactcatc aagttaaata taaatcagca actcacgacc
    15241 aaaacttttt agatagagct aaagatgaaa ttgatgatat gtcaataaat ttaaaactta
    15301 ctatacaaaa ttataaagat atagaaaatc ttaagaaaaa aatcaatgac aagggtggga
    15361 tattgacttg gtttgtatgc tgttttccag agaaaccaaa tagtttttta agttctcctt
    15421 ttgtagaaaa tagaataata ggatggtata cttattactt tggtcttgat ggatttttaa
    15481 gatgggacta taatttatgg acagaagacc cttggaaaga ttcaagttac aaattcccta
    15541 tttggaaagc tggagatatg ttctttgttt atcctggaaa agatttaaag ccagttagaa
    15601 gtgtcagaat ggagaattta agatttggaa tacaggactt tgagttattt acaatgttag
    15661 aaaaagaaaa gggcagagag tatatagaag ttgagcttat gcaagaatta ttaaataaaa
    15721 aagaaaatgc tgaaattaaa ggttttggtg acatagaatt aggatattca cttgataatt
    15781 ggggatatga taaagttaaa aaatatgtat tagagttgtt agacagagta taaaaaaata
    15841 taagagtgtc tcaaaatgaa ctttttagtt catgaggcac tctttttgta tgaaatcaat
    15901 atagtttcat tgtttcaaca atgaaaaata ggattactta tattctaaga tagcccttta
    15961 gttttttagt tgtattcaat gttttaaaac taactataaa cgctataaag tttataaatg
    16021 acaataatta tatgtaacat gatgtacttt aatttgctat aatagggatt gaataatatt
    16081 atatggaaga taacattgtt tggggggatt aatataaaaa gaataggtat tattataatg
    16141 ttttttttaa gtatagcttt tattggattt tatcttgcaa taagaggtaa cagtgataca
    16201 gttaagatgc aacgagggat aaatataggt aatgcactag aatcacctaa ggattttccg
    16261 tgggatgtaa aaatgtcaaa taagtttttt gatgatataa aagatgcagg ttttgacaca
    16321 gtacgtatac cagtaaggtt ttctgattat acgtcagatt ctgataattt taaaattgat
    16381 gaagaatttt ttaaaaagat tgataaatat gtagattatg cattggataa agacttaata
    16441 gttgtgttgg atttacatca ttttgaagaa attatgaaag aaccaagagt tcataaagaa
    16501 gagtttttaa aaatatggca acagatagct aataggtatc aaaaatatga taaaaaattg
    16561 gtatttgaat tattaaatga acctaaagaa aatttatctt cacaattact aaacgaatac
    16621 attgaagaag caataaagat aattagaaaa actaacccta aaagaactat tatagtggga
    16681 ccatataatt tctatcaaat tgattatttg aatgaattaa atgttccaaa agattctaat
    16741 atagtagttt cttttcacta ttacgaacca aatgattttg catttcaagg aaacatttat
    16801 cataatggat ttgagcactt gagcaatatt acttgggaag gaacaaatga acaaatggat
    16861 tatttaaaaa aaaggtttga tattgtagaa aattgggcaa ataaaaataa ggtaaaaata
    16921 ttcttgggtg aatttggagt aacaaaagaa gcaccagaag cttcaagaag agcttggata
    16981 aaagctgtaa gagaagaagc tgaaaaacga aacttttcat gggcttattg ggagcttgct
    17041 tctggatttg gtatatacaa tcaaattgaa gggacttggg atagagacat attaagtgca
    17101 ttaatagaaa agaggtgatt ttatgaagaa aaattcaaaa aagatactta taactttaac
    17161 tattattact aatatcgtat atatattgtg gagaattttt tacacagtac ctaaagaaga
    17221 agggatgttt gccttaatct gtgctataat acttcttttt gtagagataa tgggtatgat
    17281 ggaaatgttt gtacattatt atggtatgtc aaatattgag tatcctgaaa aaccagtcat
    17341 aagtgaagaa ctttatccac atgtagatgt attcattgca acatataatg agtctgttga
    17401 tttggtgaga aaaactgtaa atggatgtat tcatatgcag tatccagata agaaaaaggt
    17461 acatatatac atatgtgatg atggaaatcg tgaagaaatg cgtattctag cagaaaaaat
    17521 gggtgtaaat tacataacta gaactgaaag agaaggagca aaagctggga atttaaataa
    17581 tgcaatgcaa catacaaact ctcctttgat tgctacgttt gatgcagata tgataccaat
    17641 gcatgacttt ttaattgcta cagtaccata ctttttgaaa aatgagcaag ctaaaaaaga
    17701 tggagaaaaa gaggagtatg aaaaggttgg ttttgtgcaa actccacaga gtttttataa
    17761 tccagacttg ttccaattta atctgcactc tgagggaagg attccaaatg agcaagatta
    17821 tttttacaga gatattcaat tggctagaaa taggacaaat tctgtaattt atggtggtag
    17881 taatacagtt atttcaaggg aagcacttga agaagttgat ggattttata cttattctat
    17941 tacagaagat tttgcaacag gtatcttaat tcaaagtaag gggtatagat gttatgctat
    18001 tccagaagta catgcatcag gtttatctcc tacagattta aaaagtctta ttaagcaacg
    18061 tgaaagatgg gcacgtggat gtatacaaac tggtagacgt ctaaatattt tgtttaggag
    18121 aggtttaggt ttttggcaga aaataagtta tatttcttca attacttatt ggtatgcaag
    18181 tataaaacgc tttgtatata taatggcacc tattttattt tctgtgttta atgtgattgt
    18241 agtaaaatgt acattacttc aagtattggt attttggttg ccaatgtata ttttaagtag
    18301 tttatctctt aaaatatttt ctcaaaatat aagaaatact agatggacca atatatatga
    18361 aactataatg tttcagtctt taatgccagc agtaatactt gaaacgtttg caatatctaa
    18421 aaataaattt tcagtaacaa ataagaataa attagaagaa aatagaatgt ataagttttt
    18481 acagggaata ccttatttta tttacatggt cttatctatc ataggaattt taaaaatgtt
    18541 tgtagcaata tttaaaatga gttcaatgac atattcagtt gtattatttt ggttgattgg
    18601 aaaccttttt aaccttgtta tggctacatt atttatatca gggagacaac agttaagaaa
    18661 gtctgagcgt tatattgctg aaattgattt taagttaaag caaaactctt atgttctttc
    18721 ttcaaaaacg attgatatat cagaaaatgg gtttgcattt ttgctagaaa atccagaata
    18781 tatttcccca gaagaagagt tcgaggtaga gtttagagaa aaatcaggaa atgaaatgta
    18841 tatagctaat atgaaagcaa agattgttaa tgttgtggaa gtaaattcaa agtggaaata
    18901 tgcagcttat attacacata ttgaagatag tgaaatagat aattggatgt gtattgttca
    18961 cgatagaata cctactttac cgatgacaat atctaatcaa ctaggtttct ttgatgattt
    19021 gcaaattaat gtaaaaaaac gtattgaaaa gattagaaca ttatcacgaa gaagtccaag
    19081 aatcaatatg aattttcaga tggatataaa aaatacaggt aaattaagaa tagtaaattt
    19141 taattatcaa tatgtattat taaattttga gaataaaaat atttatccta aagaaatagc
    19201 attggaaatt aatgaaaata ttgtgttaga atgtgattta tgtgagggta aaatcgatga
    19261 aagaggtctc ttatataaag taaataacat tgacagcata atgcaaaatt tctttttaag
    19321 agatgaaatg atggattgga tattacaaaa taagacaatt cctgtttcaa atcctagtga
    19381 aaagaaggaa aaaagtatag atgagtttga acccatggaa tatatttagg agagatgaaa
    19441 tgaagacttt aaaagtgttt ttttgttctg caaaatatct tattttggtg tttatttttt
    19501 ttataaatac tacttttata agttcagcga caagtgataa aattttagat ttatccttta
    19561 aaaagataga gactgattta agttctagga ttacatatga agatacaggt gtaaaaatag
    19621 aaacagatag ttctaaaagt gataaggaac gttatttata tatttaccaa aatataaaag
    19681 aaaattggag tatgtataat aatttttata ttgaaattca aaataaaaat aaaagttccc
    19741 agaaaataaa tttaagtata caaagtaaga atatgcttga atttaggtta aaagaaggta
    19801 gtgaaatatt tttggaagat aaaaatataa tatattcaga taaaataaaa gaaggatgca
    19861 tagaagtacc aggtgaattt gaaggcaaaa tttatgtgaa ctttaatagc cttataaatg
    19921 agaaaagtaa tgttgtgctg gactcaagta tgctttcaaa cattgttagt tggggaatta
    19981 catttattcc atctgatgaa gaacataata ttgttattat aaaaaagata tctttattgt
    20041 ctgaagagaa attaagtttt ttacataata ttaaaataac tggcaatgaa gaagtacaaa
    20101 taccagtatt gggtcaaagt ataagtcagt atgaggtatt aggtcttaaa cgtgattcta
    20161 aaattaagta ttctcttatg ggaaaacaag ataatgtgag catatctcaa aatggtaaat
    20221 taacattaag taataaagcc gaacctggac aaattatttt acaagttaat gttgatgata
    20281 agtttaaaat tggaaggaaa atagcattaa ctgaatcttg gagtattaat aaaaaagata
    20341 aggatggtgt tccatataca ttggtatctc ctgagcaatc accaacagta caatatatga
    20401 aaaaaataaa ctttatgaat aacatcataa cgtttgttcg aattttattt gtatctctag
    20461 taataatatg ctttggaata tatttatatt ggaaaaaatg ttcaaaaact aagtaaagag
    20521 aggaggttag attgtgttat tttcaagttt aattttttta ttttattttt tacctataac
    20581 tttggtttta tattatgtat ttaggtttaa ccgaacgata cagaacatga tattgcttgc
    20641 tgttagttta tttttttatg cttggggaga gccaaaattt gttgttatca tgatagtttc
    20701 cataataatg aactatatat ttggtttact agtagataga tatagggaaa gtaaaataaa
    20761 agtaaaagtt tttttagttt tgatgtgtgt atataatata ggtgttttat ttatatttaa
    20821 atatctagct tttgcgctaa gaaatattag tgaacttatt aatactgaac taactatacc
    20881 tagtattgtt ttacctatag gaatttcatt ttttacattt cagggaatgt cttatgttat
    20941 agatgtatat cgtagacatg gagaagtcca aaagaatcct ttttatgtgg gactgtatat
    21001 agcatttttc ccacaattaa tagcaggtcc tatagttaga tatgagagtg tggcagaaca
    21061 aattctcaat agaaaagaaa cttggaataa attttctatt gggacttgca ggtttataac
    21121 aggtctaggc aaaaaagtat taatttcaaa taacatggct atagtagcag actatattta
    21181 cactatgaat agtcaaggag aaatagctgc atcacttgca tggcttggtt caatagccta
    21241 tactatgcaa atattctttg atttttctgc ttattcagat atggctatag gtttaggtct
    21301 tatgtttggt tttaaatttg aagagaactt taattatcca tatatatcaa aatcaattag
    21361 tgagttttgg agaagatggc acatttcttt gggaatgtgg tttaagagct atatatactt
    21421 tccgcttgga ggttctagag tagcaaataa agatattatg attagaaata tgtttatagt
    21481 gtggttattt actggaatat ggcatggagc agaatggaca tttgtaattt gggggatttt
    21541 aaactttgtt ttcttgatta tagagcgatt tataatgttt gaaaagattg aaaatcacaa
    21601 ttttataaaa catatttatg cgttattagt agttaacttt gggtgggtat tgtttaggtc
    21661 acctaattta aaagaagcat ataattattt caaagctatg cttggaggca atggagttat
    21721 atggagtgat tatacatata tgtttttaaa agaatactgg gttttcttta tatttgcatt
    21781 tatttttagt gtacctattg caaagaaaat caataagttt gttgttgaac aagctaaata
    21841 tagtatgata tttaacgtat tttatccaat ctcagctatt ttattgttct ttatttctgt
    21901 gacatattta gtaactggaa gctataatcc atttatttat tttaattttt agttgggagg
    21961 aaaagtatga atagattttt taaagtaaga ttaataactg caattatgtt cttaattatt
    22021 ttattcgctt tctctatctt taatatttgt tattctggaa aagatatgat acaggagata
    22081 aaaaattcag ttgctgaaaa aagtagttta aaagatacaa tatcatctat tgataacaca
    22141 gtaaatgaca aagtaatata taaaaatacg ttcattgaaa cttatggcta cttacaaaag
    22201 ttaatgggta aaaatgagtt ttcaaaattt agtgttgtaa aagatacttc tggaaagatg
    22261 cattatacat attttgctaa agctccaaat ccagttggtg tgatagcaga tagggtaaga
    22321 aaattttctg aggaaatgga aaagcaaaac actaaattag tttacttaat gactccagat
    22381 aagtatataa aaggagtgac aaaatttcca aaaggaatac cttattctta tagcaatgag
    22441 acagcagata attttttaaa tcaactaaaa aagtataatg tggactatat tgattttaga
    22501 gaaaatattt taaagagtgg tattccaaaa gatgatttat tttttaaaac agaccatcac
    22561 tggaaagtag aaacttcttt ttgggcattt ggagaattag ttgagcaact taataaaaaa
    22621 tacaatatga atttagatga aaatcattac tatagagata aagaaaatta taactcaatt
    22681 gtatatccta agtcatttct tggttctatg ggaagaaaga ctggtatctt atatggtggt
    22741 atagatgatt ttacattaat ttatcctaaa tttaagacta attatactta ttatacagat
    22801 tctaaatctc aaaaatttga attaaaagga aggtttgaag agagtttaat attatcatat
    22861 ccttttaatg cagaccttga tttaatggat ggtcaatcag ataagtattt tacatattta
    22921 ttggggaata gaccattagt taaaataaaa aatatagaaa atccagatgg attaaaagtg
    22981 ctgtttgtta aagattctct aattgtacca acagcagcat ttttttcctc tgtttgttca
    23041 tctatagata tgatagaccc tagatattat gatggagata tactagaata tgcaaaatcc
    23101 catgactatg actttgtttt tatatctgta tatccccaaa atcttacaaa agaatttttt
    23161 ccgttttgta agtaaatttc taaagaaatt ttcttataaa ataaaaaata aatggctaac
    23221 tagttaaaag ttagccattt atcttaagtg ttactctaag aaatgttttt tttaccaaat
    23281 acttctttat ctatttttcc aagtgatttt cctacagtaa ttgatgatac catagcacca
    23341 ttaacattta tcatggtacg tcccatatct agtatagggt ctattgcaag tataccacca
    23401 actaatggaa aataagaacc aagaccaact ccagagataa caactgaaac ggccattgta
    23461 gcagttcctg gaagaccagc tattccaaac gaactaattg ttataacaat taacaacata
    23521 acataaaggc tcatatccat gtttactcca gccatattgg caagtgttac agccattaaa
    23581 gcaggatata tacctgcaca accattcata cccatatttg cacctaaact tgctgtaaag
    23641 cttgatacac cctcatcaac tccgtgattt tgtactagac tttctatcgt tacaggcaat
    23701 gttcctaaac tagaacgtga agtaaatgcc agaagtaaag cttcagttga atttcttata
    23761 taagtaatgg gatttagacc atttaaagtt ataaaaatta aatgtatggt aaataatatt
    23821 gccactgata tataaagagc taatataaac tgtaaaacag atactattga gctaatacct
    23881 tgtgaagtta ttgtatttgc aagcagagca accacagcat aaggcataaa tttaataaca
    23941 gtcatagaca cacttacaat tattttgtaa gatgcttcta taaaatctat aaatggtttt
    24001 attgtttcaa agtgtttttt acttaatctc tttattgagg tagctataaa tgtagcaaat
    24061 attattgtag ctacaatatt tccatttgcc atagccatta caggattaga tggtagtaat
    24121 cctcttaaag tatccacaag agagtttatt tctttaagtt cttgattttc aactaaagta
    24181 ggcatattag ctcctacacc taacttcatt atatttgcaa cgataattcc aacaattgca
    24241 gctatagcag tagtagcaag aagcatacct aaagatttaa atgtcagagt tccaagattg
    24301 ttatctttca tatttataat tacttttata atggataaga aaactaaagg tactactaac
    24361 atttttaata agtccataaa tccagaacca aataggctat accacttgct tacttcttgt
    24421 agccaagtga cttgagtggt atcattagga aaaccagcaa aagcttgtat tatgattccc
    24481 aatatgagtc ctagtactgt agcatatatt gttctcaatg aaaattttat ttttttcttt
    24541 tcaaattgtc taacaataaa gaatgtacct agaagtaata ttatgaaaaa aatcgtttta
    24601 aaatctgtaa tcataagaaa ttgtgataaa aatgaacttt ccaatatttt ttctcctttt
    24661 atttaaaaat aaatttt
//
LOCUS       DAEPOZ010000030        24415 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.030, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000030 DAEPOZ010000000
VERSION     DAEPOZ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24415
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.030"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(371..574)
                     /locus_tag="KR440_001004"
                     /pseudo
     CDS             complement(371..574)
                     /locus_tag="KR440_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
     gene            complement(574..903)
                     /locus_tag="KR440_001005"
     CDS             complement(574..903)
                     /locus_tag="KR440_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HBH0698064.1"
                     /translation="MPQIKIRGINENDICKISKKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESMDLAFTVFEKEKY
                     YENGEHF"
     gene            1233..2159
                     /gene="fba"
                     /locus_tag="KR440_001006"
     CDS             1233..2159
                     /gene="fba"
                     /locus_tag="KR440_001006"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HBH0698065.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            complement(2294..3925)
                     /locus_tag="KR440_001007"
     CDS             complement(2294..3925)
                     /locus_tag="KR440_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0698066.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(4408..5445)
                     /locus_tag="KR440_001008"
     CDS             complement(4408..5445)
                     /locus_tag="KR440_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBH0698067.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            complement(5464..6249)
                     /locus_tag="KR440_001009"
     CDS             complement(5464..6249)
                     /locus_tag="KR440_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBH0698068.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            complement(6282..7415)
                     /locus_tag="KR440_001010"
     CDS             complement(6282..7415)
                     /locus_tag="KR440_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBH0698069.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            complement(7463..8590)
                     /gene="hadC"
                     /locus_tag="KR440_001011"
     CDS             complement(7463..8590)
                     /gene="hadC"
                     /locus_tag="KR440_001011"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HBH0698070.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKNETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            complement(8590..9816)
                     /gene="hadB"
                     /locus_tag="KR440_001012"
     CDS             complement(8590..9816)
                     /gene="hadB"
                     /locus_tag="KR440_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HBH0698071.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            complement(9831..10631)
                     /gene="hadI"
                     /locus_tag="KR440_001013"
     CDS             complement(9831..10631)
                     /gene="hadI"
                     /locus_tag="KR440_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HBH0698072.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            complement(10670..11869)
                     /gene="hadA"
                     /locus_tag="KR440_001014"
     CDS             complement(10670..11869)
                     /gene="hadA"
                     /locus_tag="KR440_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HBH0698073.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            12546..13544
                     /locus_tag="KR440_001015"
     CDS             12546..13544
                     /locus_tag="KR440_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HBH0698074.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            13906..14433
                     /locus_tag="KR440_001016"
     CDS             13906..14433
                     /locus_tag="KR440_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HBH0698075.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            14460..15056
                     /locus_tag="KR440_001017"
     CDS             14460..15056
                     /locus_tag="KR440_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HBH0698076.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEVAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKDVIDTVSISLNAPNKEAYNRVTQPKFGEKSFDYMLGFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(15121..16704)
                     /gene="asnB"
                     /locus_tag="KR440_001018"
     CDS             complement(15121..16704)
                     /gene="asnB"
                     /locus_tag="KR440_001018"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBH0698077.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDQYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
     gene            complement(17270..18106)
                     /locus_tag="KR440_001019"
     CDS             complement(17270..18106)
                     /locus_tag="KR440_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0698078.1"
                     /translation="MNYIIKKVLNSSVVLVHDAKYNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKKFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
     gene            complement(18130..19596)
                     /locus_tag="KR440_001020"
     CDS             complement(18130..19596)
                     /locus_tag="KR440_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBH0698079.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASALATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIAKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIENNSIDFK"
     gene            complement(19599..21461)
                     /locus_tag="KR440_001021"
     CDS             complement(19599..21461)
                     /locus_tag="KR440_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0698080.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            complement(21708..22571)
                     /locus_tag="KR440_001022"
     CDS             complement(21708..22571)
                     /locus_tag="KR440_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBH0698081.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFSNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            complement(22626..23558)
                     /locus_tag="KR440_001023"
     CDS             complement(22626..23558)
                     /locus_tag="KR440_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HBH0698082.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            complement(23644..24195)
                     /locus_tag="KR440_001024"
     CDS             complement(23644..24195)
                     /locus_tag="KR440_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0698083.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
BASE COUNT         7803 a         4375 c         2938 g         9299 t
ORIGIN      
        1 actatatctt atccttcggc attatccgct aaggtctacc cacttccact accgatagct
       61 tgtagtgtac ttccctcaag aggaatagtc gttgaagttt ctcctattcg gagcttacct
      121 gctgattgcc cattttaaag tacttagggt ttaaccttat accatctaac caattttttc
      181 tactttcgta actttcacac ttaggtttat ttcatcctta tgttttagtt tggttagctt
      241 taggggtttc cagcaattca agtagtattg gataggctct atcctatctc tacatataag
      301 tttccctata tgctgactat tttagctact aactcatgac taaagtcacg agtgtacgta
      361 gctatttttt aatcaaatat atatattctc tatatataac ttcttctcca ttaaataata
      421 tctcatcatt ttcagtgtct acttgagttc ctggatttga aacttcactt ccatttacta
      481 ctacagaccc ttgtttacaa tacttcttta tttcacttct acttccataa cctaaatttg
      541 aaagaatttt atctatacgt tgctttttag ccattaaaaa tgctctccat tctcataata
      601 tttttctttt tcaaatactg taaatgctaa atccatactt tctacatcta aattatttct
      661 tatactatca gttattattc ttgctactat atcttgtact tcttgtcctc tatcaaacca
      721 agctacttca acaaaaggat atacatctgc tattttacca tctcttattg caactgactt
      781 tatacattct atttcaaagt agtctcttgg acattttaca gcttcaacta aatcatttat
      841 cattttttta cttattttac atatatcatt ttcattaatt cctcttattt ttatctgtgg
      901 catagtttat ctcctttatt ttaaattttt agtgaattat catccactta attcccttcg
      961 ataacgtttg aagtggtaac ttcttggtca agacaactat tgttatcagt atctccaaac
     1021 taataaggta gttcctaccc ttatagttag cattatatag ctaattttcc taaattattt
     1081 atattcttta ttattttatt taatcctatt gtcttctatt taacattata cttgtaattt
     1141 atagttatta caatttacta tatacttttt ttgatttaac gttataatat aagtgatggg
     1201 taaattttaa aagttaatag gaggagttta aaatggcatt aattactaca aaagaaatgt
     1261 ttaaaaaagc ttacgaaggt ggatttgcta taggtgcatt taacataagt gacttagaac
     1321 aattacaagg tgttttaaaa gctgctaaag ctaaaaattc ttatgttatg atacaagctt
     1381 ctatgtcagc tgttaaatat gctggacctc atacattagt tgaaatggtt aaggctgctt
     1441 ctgatgaaat aggtgtagat gtagctcttc acttagacca tggtccaaat atggatgcta
     1501 ttaaaacttg tattgatgct ggtttctctt cagttatgat agatggttct cattttgact
     1561 ttgaagaaaa tgttagaata actaaagaag ctgttgaata tgcacactct aaaggtgttg
     1621 ttgttgaagc agaacttggt gttctagctg gaacagaaga tgatgttact tctgatgttc
     1681 acaagtatac tcaaccagct gaggcagttg agttcgttga aagaacaggt gttgattcat
     1741 tagcaatagc tattggaact tctcatggtg ctttcaaatt caaaggagaa gctaaattaa
     1801 gatttgatat actagaagaa atccaaagca aattaccagg tttcccaata gttcttcatg
     1861 gtgcatctgc tgttgaccaa aacgctgttg ctacatgtaa tgaatttggt ggaaatatag
     1921 ctggtgctaa aggtgtacct gtagatatgt taagaaaagc atcttctatg gctgtttgta
     1981 aaataaacat ggatactgac ttaagactag ctatgactgc tgctatcaga aaattcttag
     2041 ctgaaaatcc aaaagaattt gacccaagaa aatacattgg tgctggtaga gatgctatac
     2101 aagctgtagt tgaaagcaag atagatgatg ttttaggttc tgctaactct ataaactaat
     2161 ataactttta aatatttata aaagaaaaga gatgacttaa agtaattaaa tttattttta
     2221 agccatctct tttaagttgt atcaattatg tatcttataa tattttaatt atattgtctt
     2281 tttaaactga cagctaaacg tgaatatgtc cactctttat ctttctatgg atggtagaag
     2341 gatatatacc tataacttca gcagcctttg ttattgaacc atacttattt atagccttac
     2401 ttatcattat ttggtccact attttatatg catcattaag attcataatt ccatttacca
     2461 ctattggtga taaatcatct gtttcattat caatcatatc taaatttatc agcatgttaa
     2521 attcatcttc tccaattaca tttttagctg ataatactac aaatctttca atgatgtttt
     2581 ttaattctct tatgtttcct ggccaagaat attcttctaa taactcaata actttaggtg
     2641 aaacttctac ttctctattg tacttttcat tgtaaagttt taagaaataa tttactaatg
     2701 gtactatatc ctcttttctt tctctaagag gaggtatttt tactggaata acacttaatc
     2761 tatagtacaa atcttgtcta aatttaaggt tatttctaag gtcttcatga gaaatattag
     2821 tagcacttat atatctaaca tctattttta taggcttact accacctatt ctagtaatag
     2881 tattttcttg tataacccta agtaattttt tttgcatctg cattggtaac tcacctatct
     2941 catccaagaa aatagttcca ccattcgcct cttcaaaaag tcccttttta ccttttttct
     3001 tagctccagt aaaagaacct tcttcatatc caaaaaactc tgactctaat aattcatttg
     3061 gtatagctcc acagtttata gctataaaag gtttgtctct ccttgaacta tacttatata
     3121 ttgttcttgc tattatttct tttcctacac ctgattctcc atttataaat atagaagaat
     3181 ctgaggctgc agctctctta acaacttttt ttatattgtc cataatctta ctttgactta
     3241 tatactctgt gtcttctgat atatcactat attttatctg agattcaagc ttaataaccc
     3301 ttgaaatatc tctccctgtt agtactcctc ctgtaagctt tcccttagaa tcccatcttg
     3361 gaactgccgt atatgcaata agcttcccat ctgggtaaac gatatttcta taaatctttt
     3421 tcttcacttt aagcatgttt aatattattg gttcttcaac taatccttgt ctcacaaggt
     3481 ctgttatatt tctaccaagt acctcagttc ttttaaaagg taatatctca tcactgacag
     3541 tgttcatctt ttcaattacc ccatctttgt taaacacaac aacaacttca tctatatgct
     3601 ctattatatc ttcaattact ttatcagata ttagagactt ctcaaataaa taaactatat
     3661 tattttcatt attattatct ttcatattgt atatatgtgc agtcatcctt aactctctga
     3721 aagctatttt ctttttacta tctgtcagta tttctgtttt gtcaaaattt gaatctaatt
     3781 catttatatt tctaggcttt tttatatcta gaaaactaaa aatactatct attagatgat
     3841 tacagtatat tatttcgcct gattctttac ataaaatggc tggattttct acatgttcta
     3901 taagccctat taaattatta tccattaata ttcgcacctt tcattaagta atttattgaa
     3961 ctacatacaa aatttattgt ttgcttacaa taaataccat cagcacatat aatcaactcc
     4021 ttataatatt cgtcttttca agtagtttat ttcattaaaa cgtaactaag atataatttt
     4081 aatgattata gtttgttaaa aatttcattt tgcttttaat tgtattttat cacatattta
     4141 gtaattattt gtctatttaa tacaatttaa ttaaatttaa gatttattta aaaaaacaaa
     4201 gataatcctt acagaatata actcacaatt tgtaagcaat atacatataa ggattatcca
     4261 gattatcttg tattataaaa ttttattcaa acagactttt taataactga caaatacaca
     4321 ctagcaataa cttatcaaag ggttaataat tatgttacta actaataaga cttattcaat
     4381 cagtaatatt tatatagttt gtatctatta agcttttata gcttctaata aatcatcaac
     4441 gctatctaat ttttctataa gcattggaac tatttcatgt aagtctccaa ctactccata
     4501 gtcagcgatt tctaaaattg gagctgctgg gtttttgttt atagcaatta tgaaatctga
     4561 tgattgcata cctgctaaat gttgtattgc tcctgatata ccacaagcta tatataggtt
     4621 tggtttaaca gtagttcctg tttgacctac ttggtgagaa tggtctatcc atccagcatc
     4681 aacagccgca cgagaagaac caactactcc acctaatttg tcagctaatt tcttaagcat
     4741 ttcaaatcct tctggtccac ctaatcctaa accacctgat actataacat ttgcatctgt
     4801 aagagatact aaatctttct tagttttaac tgtttctaaa acagtagttc ttatatcatc
     4861 ttgagttatt ttgtagtcta atgctataac ttcaccagtt cttgtttcgt ctttttcagc
     4921 tttatccata acaccaggtc taactgtaga catttgaggt ctatggtttg gacaaatgat
     4981 tgtagccatt atgtttccac caaatgctgg acgagtttgt tttattttct tatcttctgg
     5041 gtctatttct aacttagtac agtcagctgt taatccagtt ccaactcttg aagctattct
     5101 aggtgctaag tctctaccta tatgagttgc tccgaaaagc attatttctg gttttatttc
     5161 atctattgca tcttttatta cttttgtata tgcatcagtt gtatattttt ctaaaagagc
     5221 atcatctgca acataaactt tgtcagctcc aaaagtgatt aattctttag ctaatccatc
     5281 aacatctttt cctaataata ttgcacaaag ttctgcatct acttctttag ctaattttct
     5341 tccttctcct aataattcta taactactgg agctattttt ccttctcttt gttctgcaaa
     5401 tatccatacg tttttataag aacttaaatc ttttatatca ttcatagttt gtactcctcc
     5461 aacttatata atgtgctttt gttttagacc gattattatt ttactagcta tttcatcagc
     5521 tttaccttct aagatttctc cttttcctct tggttcagga gtaaatgatc taaatacttt
     5581 agttggtgat gcttttaaac caacttcttc tttatttaca tctaaatcat caattgtcca
     5641 aacttttaca tcatgtttat atgcttttac tattttatca acagacatgt atctaggtgt
     5701 atttaactct tttactgctg ttaaaagtac aggtgtactt acttttatta attcgtatcc
     5761 atcttctaat tgtctttgaa ctattatatc ttttccttct attttgaaat cttgtacata
     5821 tgtaacttga ggaatgtcta atttctctgc aatttgtggt ccaacttgtg cagtatctcc
     5881 atctattgct tgtcttcctg caaatattat gtcatagtca cctactttag atattcccgc
     5941 tgcaatagta tttgatgtag cccaagtatc agctcctcca aatgctctat cacttaataa
     6001 tattgcatca tcagcaccca tagctaaaca ctctcttagc ataaagtctg cttgaggagg
     6061 tcccattgtt ataactgtta tagttacatc atcatgctca tctttaattt ttaaagcttc
     6121 ctctaatgca tttgcatcat ctgggtttaa tatacttgga actccatctc ttataagagt
     6181 acctgtttct ttatttattc ttacttcgtt agtatctgga acttgtttta cgcaaactaa
     6241 tattttcaaa gtctatacct ccaatttcat tagattattt attattttaa tacgtttgat
     6301 gaaattacca ttttttgaac ttctgatgtt ccttcgtata ttgatacgat tctagcatct
     6361 ctgtacattc tttcgatttc ataatctttt ataaatccat atccaccgtg aagttgtaat
     6421 gctttataag caacttcatt tgcaatttct gatgcatagt atttagccat agcagattct
     6481 tgagcaactc tcattcctgc atctctcttt tgtgctgcat cataaactaa tcctctagct
     6541 gcacaaactt ttgtttccat gtcagctata gtaaattgag tattttggaa tttagctata
     6601 ggcttaccaa attgaactct ttgttttgta tatttaacag cttcatctaa agcaccttga
     6661 gctattccta gagctaaggc agcaacacct attctaccaa cttctagagt acccattgct
     6721 atcttaaatc cttgaccttc ttttccaagt aagttttctt ttggaacttt aacgttttca
     6781 aatattaagt cagaagtttc agttcctctt atacccatct tatcttcatg agctcctgtt
     6841 gagaaaccat cccatttact ttctactata aacgctgatg ttcctctaag tcctttactt
     6901 ctatctgtaa ctgctattac tatagctaca tcacagaatg gtccattagt tatgaaagtt
     6961 tttctaccat ttaatatata gtggtctcca actaattctg ctgttgtttg ttgtccacct
     7021 gcatcagaac cagctcctgg ctcagttaac ccaaatgctc ctattaattc acctgttgct
     7081 atacctttta agtatttttg tttttgctct tcagtaccat tgtaaagtat tggtaatgaa
     7141 gcaagagaag aaccagctgt aacaaaagta gctgttgaag cacatcttct agctatttct
     7201 tccattacta tagcgtaaga aacatatcct gctcccccac caccatattc ttctgggata
     7261 ttactgctta taaattttgc atcagctaat tttttgatta attccttagg cattacacct
     7321 gttttatcca tttctgctgg taaagtatct aattctttac taacaaaatc tcttactgct
     7381 tttcttaaaa gttcttgttc tttgttatat aacatagctt cctccaattt tcatatttta
     7441 tagtaaattt attttaatga acttacgcta aactcatcat ctcagcaaaa gtttgaatac
     7501 gagttctggc ttgttcatta ttctgagttt gttggtcgat ttcaacatat aaagtaggta
     7561 ttccactatc ttctatatct tttctaacta aaggataatc gtattcttct gggtcacaga
     7621 atttcatcat acagaagata acaccatcta tatctttctt tttaacttca tctactataa
     7681 gtgacccacg ttttttctta gggtcataag ctaatgaaca tccttctatg tttgaccatt
     7741 gtcttgctaa tctctctaac gcatcatcac ctgctggtac atctgttctg aattgtcttg
     7801 tttcttgtgc taagtcgtca gctacaactg atatattgtt gtcttctaaa atgtctaata
     7861 tatctttaga atcagctaat atacctgtta ataaaacttt ttttccagaa cagacttctt
     7921 ctggcatagc atttaatttt gctattaaat ctttcactag ctcagtatgt tcttcttttc
     7981 tcataaagaa cccactttta attactaagc ttcttattga tggttttata gtattagaat
     8041 gtttataagc tacttcaaca aagtctctca tagtttttct atgttcattg taaacttcta
     8101 tactttcatg aatttttgct tcttctattt catatccaca aatttcttca agttctcttt
     8161 ttacaccttt gtactcactg attaagtatt ttacaccagc ttcaagtttt ctattttgtg
     8221 ggtgtactaa tgatatatat tttatatgag gtactgctga tttccagttt tgtcctaaac
     8281 aaattagtgt atcacacata cctggtataa taactccaga taattcatca taagcacctt
     8341 ttaatccata ttctaaacat gattgcatta ttgaacatgc aaatgcaggg aaatattgtt
     8401 tagctaaatc taattctgtt tgtcctcccc atataccaac tggaagcatt ccagctgcat
     8461 gtataatttc ttctgggcaa taaactggga aacaacctat agctttttta ccagtttcat
     8521 ttttatattt ttttacagcc gcatttggat tttcaactac ttctttcatt ttagataaaa
     8581 tagcttccat tatatctcac ctctattaag tttttttctt tcttccatta cttcaactaa
     8641 accttgaatt cttgtttcaa attgagcatt agtgaaagct ctagggtctg cttggtcacc
     8701 atcaaatcca gcatatggta atccagtttc ttcagctgct cttctttgca tttcatattg
     8761 tattaaactc ataagtttac agcttctgtt catatgatag aatgctccat cacatttacc
     8821 ctctactaaa gaatcaactc tatattttgt catacggtct aggtttacat tgttaaacat
     8881 agtactatat gctactgcca ttccatctaa atcattaact tcatattgta atgcccaagc
     8941 atgtgggtaa acactacctg tcatgttaac tccaaattta gctaatgttt tcatcttgta
     9001 tcctatatat ggccaacaag gtataccttc catcattatt ctgtattttt cttcccctct
     9061 gaaagaagat ttaccagttt tcatgttgtc ttctaattct tctataagta acttaaatgc
     9121 ttctgtagtt tcttttttac ctctagcaca aactattaca gccatgtaag taaataagtc
     9181 aaatccattc attggagaag gagaagaatc tgctggtaaa ctcatagaat acttccatag
     9241 tcttccgttt tcagctgata ttttcattac ttcttcaaac ttcttagggt caaatttctt
     9301 tcctgatata atttctagtt gttttatagc ttcttcaaat tgagctttaa tataatctat
     9361 tcttgattga gtaacttcgt cttcattatt gaaagttgta tcaatcatta ttaaaggtat
     9421 atctaattct cttgaaatat tttcatacca ttttataact tgattacata tattgttaca
     9481 gcaaagtagg aaatctggag ctggcatatc caaagcttca catccaccat tttctaaaag
     9541 accaaaattt gttcttgcat atgcacatag gtcaatagaa tatcctttag attcagctat
     9601 ttcacataat tctaaagaac cttttttagc tgctactcca gctgcttggt tttctggata
     9661 taatacgttt aagtcaaata cttctgctaa ctcttgagga aatactgatg ttgaccaacc
     9721 tacaggtctt ccttcttctt tagctttaaa tgcatttgca tattgttcag ctaataaatc
     9781 attaattact actctagctt cttttttttc agacattttc atccctcttt ttatatattt
     9841 ttcacttctt tttgtgattc ttttgcttca tcaaaagcat ataacgctgc tcctaaagca
     9901 ccagttaatt gaggtatatc aggtacaata atttctgtgt tgatttctct tgccatagct
     9961 cttacaatac cactatttct agcaactcca ccaaccataa ctacatttct ttgtactcct
    10021 attcttttta ctaggctaga aactctcttt gctactgaag tatgaatacc tgccactata
    10081 tcttcaattt ttgcattttc agataaatgt gatataacct cagactctgc aaatactgta
    10141 catgtactgc ttattgatac ttcattttga gaattcatag atatacttcc gagttcagat
    10201 acatcaacct ctattatttt tgccattaca tctaaaaatc ttcctgtacc tgcagcacat
    10261 ttgtcattca taagaaagtt taatagtctt ccattattat ctaattttaa tacctttgca
    10321 tcttgaccac ctatgtcaat aatggttctt gtctctggaa ttatgaaatt tacccctcta
    10381 gcatgacagc ttaattcact tatttgctta tcagcatctg aatagttcat tcttccatat
    10441 cctgtaacta caactttttt aatatcttct cttgcaagac ctgtcttgcc atataatttt
    10501 tctaaaactc ttgatggtcc agtagtccca gtaccagagg atattgtttc agaagctaca
    10561 atatcttccc cattctttaa gattactccc tttgatgcag ttgaacctat atctaatccc
    10621 attgtgtaca tctttagcat tctcctttta aaaaatctag aattactaat taatatctta
    10681 caactttact atctttaaag ttatttattt tctcttcatc gtagcctaaa gattttaata
    10741 cttctacagt atgttgacct acttttggtg ctggtgtata ttctttaatt ccttcatttc
    10801 taaacataac tggagtatta actaagacac ctgtatttcc gctatcgtat gttttcttaa
    10861 ataagaagtc atttgcccaa gcttgttcgt catctaataa atcttcacag ctttgaattt
    10921 tttcaaatgg taagtctgct tcttctaata aagctgacca ctcgtctaat gttttttcta
    10981 acatagcttc tccaactatc ttaactaaat cttcaacatg attaaccatt gaatctatgt
    11041 tattatatct atcatcttct aatatatatt ctctatttat aaccttacag aatttgccta
    11101 accacttgtt gtattgtatt aaagctaatt gaatccatct tccatcttta catttatatg
    11161 tagtcattaa tgggctgttt ggattttctc ttgataaagg catttcattt ccgtattgtg
    11221 ctgttgttat cattgttccc attccatata tagctgtatg gaaaagactt actgttactc
    11281 tctcaccttt accagtttga gcttttttat gtaatgctgc taaacttcct gctgctagtg
    11341 ctagacctgc atagtggtca ccaaatcctg ctgctgtatt tgctggagat gttccttttt
    11401 ccataacaga ttggctaact cctcctcttg cgaagtatgc agtatagtca aatcctggtt
    11461 tatcttttaa aggtcctttt tcaccatatc ctaatatttg agagaatatt aatcctggat
    11521 acttatcttt tatttggtca taagctatac ccattttttc taatgcttga actctaacat
    11581 tagttacaaa tatgtctgct tctgataata atttatgtaa tatttctact ccttcttttg
    11641 attttacatt aatacttaca ccctttttat ttccattttc taattcaaac atagggtttt
    11701 catcatctga tgctggagat ttaaatgttc cacccataac tcttattcca tcaccttcta
    11761 taggttcaat cttaataacc tctgcacccc agtcacctaa catttttgca caacatggtg
    11821 ctgcgatgaa acttgaaagt tctactactt taactccttc taaaagcata acgttttcct
    11881 ccttaatagt atcaattata aaatttcgat taaatcactc ataaaatact tttttctgat
    11941 attaactgtc tgatgtcaaa gttcctaatc ttatctaaaa taacttaaaa attattaaaa
    12001 ttatttttta ttgttttatt gtcttacacc tatattgtta agcatttatc gtgccaactt
    12061 tatatttaat caaaatatcg cttgatttga atgtttttta acaatatgca attgaataat
    12121 ctttttcata tcgtggcatc ttgccactat ccattgcatt ttgctattct ttctatgctt
    12181 ataaaagttt tttttatata tttttttatg aaaaagcttg acatttcaaa aaaagttttc
    12241 attgctaaac tcaaaaacac atgttatcat tgtaattaat cagcaagtta gttaactaat
    12301 ttcaaactta gataattttt taataatatt taatctaaaa aataacaatt tctttgataa
    12361 attttagagt aattattaca ttaaataaaa aattactaaa tatcaaaata acacagggaa
    12421 aacaatttca aaactaaatt tttaacctta aaaattttgt ataaaaaata taaccttata
    12481 tgctacatat tatttctaaa tgaaaaatta ccataataat ttaataatta caggagggta
    12541 ttgatatgaa aatactagta tttggagcac gcgattatga agaaccagta ataaaaaaat
    12601 ggtctgaaga acataaggat gttcaagtgg atatttatcc tgaaaacatg actgaagaaa
    12661 atgtagttaa agctaaaggg tatgatggta tatctataca acaaactaac tatatagata
    12721 atccttatat ttatgaaact ttaaaagatg ctggggttaa agttatagct tcaagaactg
    12781 caggggttga catgatacat tttgatttag ttaatgaaaa tggacttatc gttacaaacg
    12841 ttccttctta ttcacctaat gcaatagctg aattagctgt tactcaagct atgaaccttt
    12901 taagaaagac tcctctagta aagaaaaaag tctgtgaagg tgattaccgt tggatagctg
    12961 aacttcttgg aacagaagtt agatctatta cagttggtgt tataggtaca ggaaaaatag
    13021 gtgctacttc tgcaaaatta ttcaaaggcc taggagctaa tgtaattgca tttgaccaat
    13081 atccaaatag tgatttaaat gatatattaa cttacaaaga ttctttagaa gaccttctaa
    13141 aagaagctga ccttataaca ttacatactc ctttacttga aggaacaaaa catatgataa
    13201 ataaagatac tctagctata atgaaggatg gagcttacat agtaaatact ggccgtggtg
    13261 gtttaattaa tacaggggat ttaatagaag cactagagtc aggaaaaatt agagctgctg
    13321 cccttgatac atttgaaact gaaggattgt tcttaaacaa aaaaatgaat cctggagaat
    13381 taactgaccc agaaataaat aaacttcttt ctatggaaca agttatattc actcatcacc
    13441 ttggtttctt cactagtaca gcgattgaaa atatagttta ttctagttta agtagtgctg
    13501 tagaagttat aaaaacagga actgctacta atagagtaaa ttagtatttt ataaatatat
    13561 tttttcatta attagctatt atctaacaaa atagccttta atttcccaaa tataagagag
    13621 tcagctcagt atatattgag tagactctct tatattattt tttatattaa acttgtactc
    13681 ctcttattta taccaaatta aatttatatt aacttactct aactactcct actctataca
    13741 ttttacctat ttttactttt ctaattatat acatttgaat gggtacaatc tgtattttac
    13801 aaaaaaaaca cctaacaaac tatgaaaatt gtatgattaa tattaatata tttataagaa
    13861 aacataacaa ttatgttagc tctggatttt tagggaggga gtatattgga aaaaatgaat
    13921 aaagttactt ctaaaaaaac tacctatatt gttacttcag cattatttgc atccataata
    13981 tgcctgacaa tagcatatat attacatatt ccagttggtg gaaacaatgg atatgttcat
    14041 ataggagatg cttttattta tctagctgca actattttgc caacaaacta tgctatagct
    14101 gcttctgcaa ttggtgctgg tctagctgat ttatctacag gtgctgctat ctgggtaata
    14161 ccaactataa tcataaaacc aatacttgta ttatttttca cttctaaaag tgacaagata
    14221 ataaataaga gaaatatagt tgcttcggtt gtagcaggta tagtaggatt agttttatac
    14281 atgtttgctg aaggtataat tattggaagt tttacaagtg catttgtaat gagcttactt
    14341 ggcttattac agccaatagg aagttttatt gtttttataa tcttaggtat ggcacttgat
    14401 aaacttgatt ttaaaaaacg ttattttaat taagaattta aatttatgga gggaaaatta
    14461 tgaatatctt atatacaatt gaaaatagta tctatgtaaa tataactaat acatgtccat
    14521 gtagttgtgt tttttgtata agaaacgaaa aagatgaagt tgcaaatagt ggtagtttat
    14581 ggttggagca tgagccaagt gtagatgaag taaaagaagc cttcaataaa tacaatttag
    14641 acgactatga tgaaatcgta ttttgtggat atggcgaacc attaatgaga ataaatgaat
    14701 tgatagaggt agcaaagttt ataaaagaaa aaagtagcat aaaaataaga ataaatacaa
    14761 atggtctaag tgatttaata cataacaaaa aaacagctat tctgttaaaa gatgttatag
    14821 atacagtatc aataagtctt aatgccccaa ataaagaagc ttataataga gttactcaac
    14881 caaagtttgg agaaaaatca tttgactata tgttaggttt tgcaagagat tgcaaaaaat
    14941 atataaaaga agtagctttt tcagtagtag atgaaatatc tccagaagaa atagaagaat
    15001 ctaaacaact ggcaaaaaaa cttgatatac cattaagagt tagacataag aattagcaag
    15061 taaaattata aaacattaag aatcacctga atggtctttc aggtgattct tcctcttgta
    15121 ttaacatttt acaaaataga tttcatacca cactaggaat gtgtaaatca tccaaatctt
    15181 tctccaatta tcagagtttc cactaatgta gtcattaaaa atctcattaa tctcatctat
    15241 gttaaagaat ttatctgcta tctcactatt gaatttatct cggatatcct tattatattg
    15301 gtcatcagcc atccatatac gaattggcac aataaatcct aatttatttc taaaagcaat
    15361 ctcttctggt aatgtttttg atgcagctgt acgaaaggca attttattct cttttccttt
    15421 cactttatat tctgatggta ttctggatgc aatatcaaat attcttgtat ctgtaagagg
    15481 cattctgact tccaatccaa ctgcagtact cattttatct atattaaaat aaatatctcc
    15541 ttccaaccat atttgaatat ctacatttga catgttagta agtgtatcta gttcttctgt
    15601 ttcttcataa attcctttta ccacttcaat aggaagcaca tcaggattgt atttcttaag
    15661 aatttttctt ttctcaccct cattcataat gtttgtattt ccaatataaa atgtcttcaa
    15721 attctctcca taacactctg ccttatggta tatattatat ccaccaaaaa actcatctgc
    15781 tccttctcca gagtaacaga tatttgtgtt ttcagctgct gccttgcatc caatagcaaa
    15841 aacgatagca gatgcatctc cagttggttg ctccatatgg tacattacat aaggtactga
    15901 cgcaaaatac tcttcaggtg taattatata tctcgaattt ttacacccaa gaatattagc
    15961 tgtctgttgt gctaattttg attcatcaaa acgctcttcc tcataaccac atgaacctgt
    16021 tttttctata tctgacattg ccaatatata agatgaatca acacctcctg ataagaataa
    16081 ctctgcagtt tcattctctg attttacctc tggcataatt ttctgaagtg ttgtatgaat
    16141 ttcatccgtc cattcctcaa tagacttgct gtggtcagga tgaaattcag gtttccaata
    16201 acgaccaata catactgttt tattctgata gacaaggtaa tgtcctggca taagcttctt
    16261 tactccacgg aagaatgtat tttcacctgc tacatatgtg aaagacatat aaatctgtaa
    16321 catgtcttca tttaactctt tcacaaatcc tggttgctcc ataatcttac gaatagatga
    16381 accatagaga agcttcccat tttcagtctc ataataatag aaaggtttta ctccaaactg
    16441 gtctcttagg cagaagagtt tcttttccac agtatcccat aatgcaaagg caaacattcc
    16501 atgcatgtga tttgccatat catatcccca tgtcttatat gcttccacta aaattgctcg
    16561 ttcacgctca tctctacata atgaagaatc gattccaagt tcttcacaga gaactttcca
    16621 gtttcgaata tgtccattgt attctagaat ttttatcata tatgtgtctt ttcgtgttaa
    16681 atacttttct tctaaattta tcatatgtgt atcctttcgt attaaaacat ttaacttcta
    16741 aatttattaa atgtttatat atatttattc acttataatc actgaaaaat cgactttttt
    16801 caaagtaaat tctttttgaa ttagtactaa tctgctcttt attaatggca taaatatgat
    16861 tatcaagtat tatcagtgtt ataacattga attttttatt cttcataaat attgccccct
    16921 ttatattacc aactaaatat aatatatctt atgttactta catattcttt aagaaattct
    16981 gaagatttta taaagttttt tgtattataa ttaattaaaa ataaaattct agttataaaa
    17041 catattttcc attaaacaaa aaaatagtta aaataaattc ataaccacat aaacttattt
    17101 taactatttg tatattttaa ttataaatat taacttaaat ttttaacagg atacactcat
    17161 aaaataaaca taatgaacaa caaaataata tgtctcagtt tctttatata ctttaccttc
    17221 tttcaatttt ctaccatgta gacgtataag ataatattag taattcactc tattgcttaa
    17281 ctcgtgcgat atggacaacc aagaaagcta tttcctcatt tgttaacttt tttccatatt
    17341 tcttttctaa aaaatctcta acttttaatg caattaacat ttcttttgga tttgaccttt
    17401 tcatttgatt aaataataaa tcatcaccac tgcttaacat actatctgaa aaaaatcttt
    17461 ccacaaaaaa ttttatatgt gttataaatc tcatataatg catactcttt ttatctaagg
    17521 ttctattcaa agaaaaaaca aaaatattaa ttatttctcc aattactttg gcatacttaa
    17581 tcgtatcata tgaatttcca tctgaagcca tagcatttgc aaaatggaaa gcaatgttag
    17641 ctgcttcttc ctctggtaat tgaatgccca atctctcctc taccatataa attgcaacca
    17701 ttccaacctt aaactcattt ggataataat tttttatttc ccaaaatact cgatttgtaa
    17761 tttttattcc tttacgcatc ctttcaattg caaagttaaa atggtctgct aatattaagt
    17821 ataagttttt attaaaagat gagttcaata acttttctgc ctctactatt atttcctgtg
    17881 ttatctttaa aatgtcgaca ggaatatcat ccattaattc taaaaatttt tttgattttg
    17941 tattttcaac agggacaaac atttgattat cattggaacc acaaatatat tctcctgttt
    18001 ttttaccata tccaattcct ttccctagca aaataaattc attatacttt gcatcatgaa
    18061 ctaaaacaac gctcgagttc aaaactttct ttattatgta gttcatttta tttgctcacc
    18121 ttctattggt catttaaaat ctatactatt attttcaatt acttttttat accaataaaa
    18181 actatctttc ttatatcgat tatatgtccc ctttccataa tcatcagcat ccacataaat
    18241 atatccataa cgttttgaca tttgtttcgt tgattcactg accaaatcaa tacaacccca
    18301 tgaagtgtat ccccaaagtt caacgccatc ttcgataatc gcatctaaca tacatttaaa
    18361 gtgttctttt aaataattaa ttctatattg gtcatgaact tttttatctt cagttaaaat
    18421 atctttagct cccaatccat tttcaactat aaataaaggc tttctgtatc tatcatataa
    18481 ttcaatcaaa gatatacgta gacctattga atcaatttgc caaccccatt cactcatttc
    18541 taaataagga ttttttacag ctaataacgt attcccaggt gtaatatcta atccagttgt
    18601 atcagctgcc tcacaagaag acatatagta agagaatgaa ataaaatcta ctggatactt
    18661 tttcataatt tcatcatcat gttcttcttt tgctatttga atgttattat ttttaaaata
    18721 cgaaagtaaa tatactggat actcaccaaa aacctgcgta tcactatatg cataaatact
    18781 tctcattttt tgttgagttg ccaaaacatc ttctggtcta catgtatatg gataatacgt
    18841 taattttgtt aacatacacc ctacttttga atttggaata atctcatggc aaatttttgt
    18901 tgcaagggca ctagctacaa attgatgatg catagcttga aaaataactt cctcaaagtt
    18961 tttatcctta aaacggtctc gaactaaacc accagtagtg tacggatgac gaatcataga
    19021 atcaacttca ttaaatgtaa gccaatattt cactttgttt ttatatcttt cacagatagt
    19081 ttctacatac ctaacaaaca tattgataac ttgtcgtgaa taccatccat catagtttaa
    19141 aacgatattt aatggtggtt cataatgtga catagtaaca agtggttcaa ttccataact
    19201 tatcaactca tcaaatacat catcataaaa ctgtaaacct tcttcatttg gctctttatc
    19261 atctccattt ggaaaaatac gagaccaagc aatactcatt cgatatgtct taaatcccat
    19321 ttctgccatt agagcaatat cttctttata gtgatgataa aaatcactac catgacgttt
    19381 tggataattt acttcatctt caggatgtgc taatccttct tctatatctt ttgttgtgat
    19441 ttcattatta gatttatagt ctttcacatc tgcatcaaag tgtgcctttg ctacatctga
    19501 tacagaccac cctttaccac ctaaattcca accaccttca aattgattag cagctgtagc
    19561 tcctccccaa agaaatcctt ctggaaactt tctcatattt atattcctcc ctttacatca
    19621 aacaagatac ttcctttttt tacattgcca taagactggt ttaacacttt ataagcataa
    19681 tctgctccat tattgtttac aatcataaca gttgaatcta aattagcatg tgagattttg
    19741 tctaaatcaa attgaattaa taaatctcct tcttttacat tatcgcctgt cttaacaaat
    19801 gttttaaatc catctccatt catacttaca gtatttattc caatatgaat caaaatctca
    19861 gccccatttt ttgttcttaa accaattgca tgttttgttt caaataacat cacaacttct
    19921 gaatcacaag gtgcgtataa atttccattg gtcggtataa ttgcaactcc atctcccaaa
    19981 gtcttacttg caaatagttc atcattaacc ttacttaaat ctattacttt tccttctact
    20041 ggagttacta agatatttac ttctttttct tcttctagct cttcttcaac aacatctcca
    20101 attgatgact ctactgtatt tgtatcttcc ttccaaataa tcagtgtaat aataaacgag
    20161 cctaatattg caataacaaa accaatactc gcaaaaatca aattgtttcc attgtttggg
    20221 tcaataaaca tactcatact ggcaagccct ggtccaacaa ttacaaatcc ttttactgca
    20281 aataatccta aaaatgtacc agaaattgca cttgagaata caacacctaa cattgctctt
    20341 ttatgttgta aagtaatacc atataaagct ggctctgtaa ttcccatcaa ggcagaaatt
    20401 cctgcagata atgctgtttg ttttaatgtt tcatcttttg ttcgcaaggc tactgcaaaa
    20461 caagcaccac tttcagaaat attgtgggct aatgaagatg tcatatataa agcttcataa
    20521 cccaactttc caattgtatt aattgaataa ggtattagtg ctttgtgcat tcctgttgca
    20581 accataaatg gaagaaaagc agcaagtaat cctacaacta agaatcccat tttgttatat
    20641 aagaataaaa taatggttgt aaaaattgta ccaacgttat atcctaaagg acctaaaata
    20701 gttagagtaa ttggaacagt aattgccaaa gacatcatag gaacaaagaa agttcgaatt
    20761 ggttttggtg aatacttgtt aaaaattttc tccattgttg ccaaaaaaga cacacataag
    20821 atagccggga aaacttgtgc attataggca atgtttggaa ttgtaaatcc aaatagttcg
    20881 acaccttcac ttaaagcagt tgttgttgct ggaagcaata agtatcctac agcggcaata
    20941 gctactaaac gattacattt caatacattg gcagtagtta cagctaccat aagaggtagg
    21001 aaatagaatg ttgcatctga aatagcaact aataatgtat atatagtaga atctgatttt
    21061 aaaatattaa acattgctaa taacatcaac agtgatttta aaataccagc tcctgcaata
    21121 gcaaaaatca gtggttgaaa aatattaata ataaattcta taaatcttgt tcctaagttt
    21181 attttttctt cagtttttac agtactatca tttgtatcta aagggcaaac ttttaaaatt
    21241 tcatcataca catcaacaac actatttcca atgacaactt gaaattgtgc cccattaact
    21301 atactcataa caccttgtac tttttttaaa gcatcctgat tgactttact tctatcaatc
    21361 aacttaaagc gtagtctagt tgaacagtgt tctaaagcta aaatattttt tgaaccacca
    21421 acttgttcaa taatgtcaat ggcagttttt ttataattca tcttgtaatc tccttctcca
    21481 taatttttta tgaatggcca ttcaagatgc aaaaaaggca gaacctatat aaacaccttg
    21541 aaaaatcaaa gcaatatatt cgatcctgcc tgcattacca gtaacatgtc ttctatttat
    21601 actattttgt attattatat taacatttgc ctaaaagaat tgtcaatact tttttaaatt
    21661 ttctgatact aactaatagc acctttataa ttcatttttt atgtcaatta aagtaacagt
    21721 agccacccac caattagtat caaaattgaa cctggtattt tttcttttat attttctttg
    21781 aatataaaat gagcaagtat taaagttatt atcatagata cttgagaaaa tggctctgct
    21841 atactaagtt ctagattatt taaagcaatt aataatgcta aataagaata tccattaata
    21901 gcaccactta aaattgaaag ccccttttta ttactaaaaa tctcacaaat tattttttgt
    21961 ttctttttaa atgctaaaaa gataaataaa tttaaaccta cgaaaaaata cagtatagtt
    22021 gaatataata taggagatac ttgtacaaaa aaagccttat ccaatactct tcctactgac
    22081 tgcaaaaata tgtataaaaa cattaatcta catggtaaat tgttaaaaat tacagagaca
    22141 ttctttaaaa aagagcttac tttttttaat ataaacactc caccaatcat aattatgatt
    22201 cctgcaactt ttgctaaact taaaccttca cctaaaaata taactgataa aagcagtaaa
    22261 aacaatgaat ttaaactatt tattggagta acaagtgaaa cttccccagt tgctaaagac
    22321 gttacatacg ctactgaacc aattgaatag actaaactac ttatataaca tggtattaaa
    22381 aacgaccaat cactaactct atccattatg ctaaatggaa gtaaacatac tccccctata
    22441 aagaaaaata gaaatgatga agctatattt ttataaatat ctccttcttc atcacctaat
    22501 aatcttacaa ctactctttc aaaagctaat agcactactc taattaataa tgcaaaataa
    22561 gataatatca tttttgacct cgcttaaaaa ttaatccgac ttattgttat attaactgta
    22621 taagactacc atataggtaa gtctttggga ttttttaata ttttatgttt aaatacaggt
    22681 acattaagtt tttcacctag tttacggcat tgctctttat aggtaccttt taatttatca
    22741 tgaaaaacta tacaatcaat tccaccttca atactattta tacacttttc tagtttctca
    22801 aatcccgatt catcatcata cattccactt acaatccatt gatttggcaa tacatgtcca
    22861 attgatatgt tatattcttt attttttata atatctgaga aagatttctc aaaatgtacc
    22921 cctccaacac ataataaagt cttcaaaggt tcatcacaat caaatactct aattaatgat
    22981 tttgccaata cctgtattgc tatactatta ttaaaactat cactactact tcctatctca
    23041 atatccaaca taggtacaga atattctgta attaatttag attcttcacc atatgttgtt
    23101 ccagaccagt gagttgcttc tgttaatgta gtaaattcat caagtagatt ttcatttttt
    23161 atatcctcaa tagcattgat tagattttta aagtatcttg gatttgaatt tccaaattct
    23221 cctgttggaa catctccaat tgtatgagct gttaggatat gttctggagc attttctccc
    23281 tcatgccaat ttacaactcc tgcaaaatca taatctctga aatgtctatt taaaattgga
    23341 agataatatt tataattatg acttattaca tcttttgttt ttacaaaatg aaataagtta
    23401 ttgtttttat ccttaaattc taatacaaaa ttatcatcaa taacaatatc agtttgattt
    23461 aaatccatta tttcttcaca acactttatc acattactgg ctactgggtc tttctttaaa
    23521 tcactacaaa agaaaaaaac tgctttttta ttattcatcc ataaatcaca ctcctataac
    23581 ttaaatataa tatatcttca tacctaaaat catctaaata tcactctaat tccattaaat
    23641 tatttatata taatatacta ccatactcat tttagctata cctttgcctg agtttctata
    23701 tatatgtgat acatcagctt taaatcttat agaatttcca ctagatacaa tgtaatcttc
    23761 tccatttatt ttaatggtta cttcgccact aaaaacagtt ataaactctt gtgttcctgg
    23821 aagttcagca tttgaatcta aatttccacc ttcttctatt tcaatcataa atatctcaaa
    23881 tcttcttttg gagtcaaatg gaaatattgg ataacttata tactttccat tatcttcaat
    23941 aaaatggtct atatcacttt tttgaatcaa ttctatgtca ctttcctgcc tctccatcaa
    24001 tgatgtaaat gagattttta gtccattagt tattttcttc aaagtagata tagtaggatt
    24061 tacctcacac ttttcaattt gagatagcat actcttactt acaccactaa gttgtgcaat
    24121 cttgtctaaa ctaagatgtt tttctttcct tatctttttt aaattttcag cgattatttc
    24181 atttaaactt tgcataaata ccccctaata catactaata taagataaat tattttgatt
    24241 attaaataaa cttagtaatt taatagaaac tttttatcca ttatactgta cattatgttt
    24301 aatatacatt acttttttta gaatgtaaat agttttctga aattttgtga aagtgaacaa
    24361 ataaaaaact agtaacctat atttttacaa ggctactagc ttttattata ataat
//
LOCUS       DAEPOZ010000031        23167 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.031, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000031 DAEPOZ010000000
VERSION     DAEPOZ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23167
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.031"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(356..1447)
                     /locus_tag="KR440_001025"
     CDS             complement(356..1447)
                     /locus_tag="KR440_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698084.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEMTVMKEDISEV
                     KQEMTVMKEDMSEVKQEINVLKGDMSEVKQEMTVMKEDMSEVKQEINVLKEDMSEVKQ
                     EMIVMKEDMSEVKQEMTVMKEDMSEVKQEMTVMKEDMSEVRQEINIMKEDISEVRQEM
                     TVMKEDMSEVKQEINVLKEDMSEVRQEINIMKEDMSEVRQEMTVMKEDTSEVKQEINV
                     MKKDMSEVRQEITVMKEDVSEVKSEVNFMQNKINNINEDMNGIKDEVSIANEKLYGIE
                     IKIDSLECEDKSMKEIQIEQNNILGSLLHNSEINKATHDNIEHNIAYIKGDTNSIKED
                     IAEIRRDLNLVELATSKNWSDIVKLKSVK"
     gene            complement(1677..2762)
                     /locus_tag="KR440_001026"
     CDS             complement(1677..2762)
                     /locus_tag="KR440_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBH0698085.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASVGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            complement(2798..4111)
                     /locus_tag="KR440_001027"
     CDS             complement(2798..4111)
                     /locus_tag="KR440_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBH0698086.1"
                     /translation="MIAIKSKYLISSADEIYTNSAAVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            complement(4112..6403)
                     /locus_tag="KR440_001028"
     CDS             complement(4112..6403)
                     /locus_tag="KR440_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBH0698087.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDTKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGRTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            complement(6393..7187)
                     /locus_tag="KR440_001029"
     CDS             complement(6393..7187)
                     /locus_tag="KR440_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBH0698088.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLESELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVVRMYNSIGGECDGK"
     gene            complement(7226..7672)
                     /locus_tag="KR440_001030"
     CDS             complement(7226..7672)
                     /locus_tag="KR440_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBH0698089.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVDGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            complement(7744..9150)
                     /locus_tag="KR440_001031"
     CDS             complement(7744..9150)
                     /locus_tag="KR440_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0698090.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     VRGTIKNRMEGHKWNSLY"
     gene            complement(9168..10538)
                     /gene="hydA"
                     /locus_tag="KR440_001032"
     CDS             complement(9168..10538)
                     /gene="hydA"
                     /locus_tag="KR440_001032"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBH0698091.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKMAREKNIDMIVETCPQYLLLEDNLYNKN
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISVNKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            complement(10671..11858)
                     /locus_tag="KR440_001033"
     CDS             complement(10671..11858)
                     /locus_tag="KR440_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBH0698092.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGVNAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKSV
                     YGK"
     gene            complement(11985..13199)
                     /gene="dpaL"
                     /locus_tag="KR440_001034"
     CDS             complement(11985..13199)
                     /gene="dpaL"
                     /locus_tag="KR440_001034"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBH0698093.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            complement(13562..14233)
                     /locus_tag="KR440_001035"
     CDS             complement(13562..14233)
                     /locus_tag="KR440_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH0698094.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            complement(14316..16532)
                     /locus_tag="KR440_001036"
     CDS             complement(14316..16532)
                     /locus_tag="KR440_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBH0698095.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHNIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFRGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLIVEEAEKLLETSKEDLYLDNGYVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGLTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVNSYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            complement(16535..17005)
                     /locus_tag="KR440_001037"
     CDS             complement(16535..17005)
                     /locus_tag="KR440_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HBH0698096.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIKEAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            complement(17067..18461)
                     /locus_tag="KR440_001038"
     CDS             complement(17067..18461)
                     /locus_tag="KR440_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0698097.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQLAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            complement(18534..19895)
                     /locus_tag="KR440_001039"
     CDS             complement(18534..19895)
                     /locus_tag="KR440_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBH0698098.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            complement(20193..21515)
                     /locus_tag="KR440_001040"
     CDS             complement(20193..21515)
                     /locus_tag="KR440_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBH0698099.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            complement(21871..22911)
                     /locus_tag="KR440_001041"
     CDS             complement(21871..22911)
                     /locus_tag="KR440_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0698100.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
BASE COUNT         6911 a         4666 c         2538 g         9052 t
ORIGIN      
        1 tagtaatatt atttttatag taaaaatttt attcatatta ctatttataa tttttataga
       61 gcaattttag tgccaatatt tgcatacatt tgtatagtat aaaattttta tgttctcatt
      121 gataaaattt tacttatttc aagcgtttta ttcgatttta tcatctaaat tttaggtttt
      181 atttttataa attaattgta tttacataaa aaaaaagacc taaaaacaaa tttttaacgg
      241 tcttttctca attttttatt ttcatttcta aaatttataa taaatagtaa gaacactata
      301 aattctttta tataaaattc ttagtttgtg gatgataaat tagtgaatta aaatactact
      361 ttactgattt tagtttgaca atatcactcc aatttttact agtggctaac tctactaaat
      421 ttaagtctct tcttatttca gctatatcct cttttataga attagtgtca ccttttatat
      481 atgctatgtt atgttctata ttatcatgag tagctttatt tatttcagaa ttatgtaata
      541 aacttcctaa aatattattc tgttctatct gtatctcttt cattgattta tcttcacatt
      601 ctagactatc aatcttaatt tcaattccat ataatttttc atttgctatg gatacctcgt
      661 ccttaattcc attcatatct tcattaatgt tatttatttt attttgcata aagtttactt
      721 ctgatttaac ttcggaaaca tcttctttca ttacagttat ttcttgtctt acttctgaca
      781 tgtctttttt cattacattt atctcttgtt ttacctctga tgtatcttct ttcattacag
      841 tcatttcttg tcttacttca gacatatctt ctttcattat atttatctct tgtcttactt
      901 cggacatatc ttcttttaat acatttatct cctgttttac ttccgacata tcttctttca
      961 ttacagtcat ttcttgtctt acttcggata tatcctcttt cattatattt atctcttgtc
     1021 ttacctctga catatcttct ttcattacag tcatttcctg ttttacttcc gacatatctt
     1081 ctttcattac agtcatttcc tgttttactt ccgacatatc ttctttcatt acaatcattt
     1141 cctgttttac ttccgacata tcttctttta atacatttat ttcctgtttt acttccgaca
     1201 tatcttcttt cattacagtc atttcttgtt ttacttccga catatctcct tttaatacat
     1261 ttatctcctg ttttacttcc gacatatctt ctttcattac agtcatttct tgttttactt
     1321 ctgatatatc ttctttcatt acagtcattt cttgttttac ttccgacata tcatttttta
     1381 tgctaactac atcttcattt atagtatata ataactctaa aatcttctct tgcacttgca
     1441 tcaccatccc tattaattat tataacattt tacacaaatg ctatacaatg ttaacatata
     1501 tatcagaatt attaaaattt taattaaaca aaattgattc actttattac ttcaaaacta
     1561 attaatatct aaaataacta caaataaaca aaaataaggt caacaacaaa aaaggcttat
     1621 ctctttgaga tagcctttct tatttttatt taaaactttg ttttttataa aataatctat
     1681 acattcagct ctgaacatga aatttttctt ccaagtgctt ttggtttaca aactgatata
     1741 caaagtccac aatatctaca ctcttcttca ttcaattcca tcttatcatt caattttctc
     1801 gcttgatatg cacaaactct ctcgcacatc ttacatttag tacatttttc ttcatcataa
     1861 gtaaactcaa agctaagtct ttcattaaca tctcccatat gattatgtac tataccacaa
     1921 gcattttcta tagtattata tcctaatcta ttaagattag attttaaatc atcatgtatt
     1981 ttagatatta cttgtgcgcc ttttaaaatt actactgaac atataccaac aaagtcagca
     2041 cctgccataa tcatttctaa ggcatcatct ccacttacac atccacctaa gccaattatt
     2101 ggtatattaa cttgtttttt tatttcatat attctctgta aagttattgg cttaatagtt
     2161 tctccactta aatatcctat tccatttcca cctaataaag gttgccctgt ttctatgtct
     2221 attctaaatg ctggtcctac agagtcacaa gcagttattg catctgctcc tgcttcttct
     2281 aattttttag caaaatcgcc tatctcatct atcataggag gaagtttaac tattacaggt
     2341 atatttactc tttcctttgc atctttaagc attggtatca aatctctata gtcataagat
     2401 actaattcaa taaagtcagc tcctgcattt gcaactttct ctactaattc gctactttct
     2461 tcaattgtat gacctactga agctatacag acagtcccat ctcttttcat ttgtggtatt
     2521 tcaaaatcta tccattgctc tggctcaaaa tctgtccatt tttcattatt taaaagagca
     2581 ttagctgtat ttctaaccat atgtggtgct ggatttatag ctctttcttt tcttatagtc
     2641 ttagtcacaa ctgcccctgc tcctgcatca cttaatatct tgcatccagc tgcactatat
     2701 gttaatggtc ctgaacctat tataagtggt gattttaatt ctttacctaa aaaattatac
     2761 attatctgct tccccttttc atatatattt acattcgtta aaatttccaa aattcacaag
     2821 caacttcttt catttcttta acaatagctt ttttatctaa atcaactaac tgtttttctt
     2881 ttaaaatatg tctaccatct atgtatacat ttgatataaa ttctttttta ccctgaacta
     2941 ctatctggtc aaatatatta tcaagagtca caggagtttt aaattcatct tccataacta
     3001 ttatatcagc cttattacca actgctattt taccagaatt ttgaagtcct aatacataag
     3061 caccatgctc tgttgccatc ctaaatacta aatttgctgg cattactgat gcatcttctt
     3121 caactgattt atgcattaaa aatgcacctc tcataacttc aaaaaaatca tttatatatc
     3181 catcagtacc cagtgccact tttatgcctt ttttaatcat ttttggtact ggagaaaaac
     3241 ctccacctac ttcacagttg ctaagaggca tatgaaccac ttttacacct ttttctttta
     3301 atatatctat ttcttcatca tttactttta cacactgaga tgctaataca gtttcatcca
     3361 atatatctaa atcattatag tagtcaactg cttttttgcc aaaatgtttt tcagcatagt
     3421 ttggctcata tatactctca cataaatgaa attgcattgg tgcatttaat ttttttgcat
     3481 cttctttagc tttcttaata aatctatcag aacatgtgaa gcttgtatgt gtacacatta
     3541 ttccatttat taatttacta ttttctctac tccactttat taaattagaa ttttcatcta
     3601 aacatctaag tccattttca aagcttattc tttcacaact ttctaaggat aaaatcgctt
     3661 tcatgcctat ttcttctagt atttttcctt gctcaattaa agttccttct tctgtatttg
     3721 gagcttctag ggtatcacaa aaagctacca caccagattc tataagttct attgctgatg
     3781 ctttagttgt tgccttcact tctttcaatc caattctatt ttctataaat ggccaccaat
     3841 agtcatttaa aaaaccttca aagtcagtaa aatgtacatt tgccggtatt cccctagaaa
     3901 gaactccata ttgatgcata tgaccattga cgaatcctgg cattattata gcatctgatt
     3961 tatctattat ttcttcaata tttttatatt ttatttcaac ttcttcattt ggcaatatat
     4021 cttttataat gtcattttct actacagcag cagaatttgt atatatttca tcagcagaag
     4081 atattaagta tttactttta atagcaatca tttatttacc tccctgattt aattttttca
     4141 tggccatata aactttctca gaagttatag gaagttctct tatacgaact cctgtggcat
     4201 tgtatattgc atttgctatt gctggtgcag gtgtatgaat aactatttct cctaaagatt
     4261 tagctccata tggacctgtt ggctcataat tatctacaaa atcaacaatt atgtttccta
     4321 tatctttttt tgttggaact ttgtattgta atagattaga agtttgaagc tttccatcag
     4381 aaccatgtct agggtcttca tacattgcaa gccctatacc ttgagttaca ccaccttcaa
     4441 cttgtattct agcaagtgca ggattcataa ctgtaccaca atctgtagaa caagcataat
     4501 ttattacttt aaactcccct gttgttttat ctaactctat ttcagcaaat cctgctaaga
     4561 atggtttagc actctcttgt ataccataag attcagatga atataaaact tcttgatttc
     4621 caccaccaac agattctttt cctaattcac ttaacaaaac aaacttctct acatcttcac
     4681 tataacaaac tctgtcttca tatagtatta aatcatctat tggtaaatcg agtttcttct
     4741 gtgctacttt taatatcttt tgttttaaac tttcagcaga ttttattgta gcatttccag
     4801 ttacataagt agtacaagaa gcgtatgcac cagtatcata tggacacata tctgtatctc
     4861 ctgtataaac ggaaattcta tcaatagatg tattcaatgc ttttgcagct atttgtgcaa
     4921 gtattgtgtc agaaccagtt cctaagtctg aagacccaga aaacaactta taagttccat
     4981 cttcatccat cctcatattt actattgcca tgtctatgtt ggctatacca gaaccatgag
     5041 ttcccacaga cattccaaca gccctcacct tattgtttcc aatatcccta acagggtact
     5101 tttcatccca tccaataagt tttttacctc taattataca ttcatcaagt gcacaacttc
     5161 ttattggaaa attcattatt ccgccttcag tttgtttttt tataatattc tttaacttta
     5221 tttctgttgg gtccatatta agtttatgcg ctaattcatc aattgcacaa tcaagtgcaa
     5281 atgtaccttg tgttgctcca tatcctctta atgctcctcc tggaactaaa ttagtatata
     5341 cagttctccc atcaaatttt attgcaggta cattattata tgttggaagt acattatgac
     5401 ctgattcaga gcaaactgaa ggtccattat caccataagc gcctgtatta ttcaatgctt
     5461 tcatatctat agcttttatg tttccttctt tatctgcccc aatttttata tcaaaaaaca
     5521 tctgatgtct agtattagtc attgcaaaag tttcttctct agtataaatt agttttgctg
     5581 gtctttttgt catccaagtt acaaatgatg catatatctc tgttacagct atgttttttc
     5641 caccaaaacc tccacctatt cttggtttta ttactctaac ttttgaaaga ggtattccca
     5701 gtgctttaga aacctgtctt ctcatatgat aagctgattg gtttgcagaa gttactacta
     5761 atctgccatt cgtatctata tacgtataag ctctatgagt ttccatcata caatgggctt
     5821 gtgattgagt catataagaa tgttctacta ttacatcact ttctttaaac tctttttcta
     5881 catccccttt acccattttt tctctcgata caatattttt tgtagcatcc aatccaaaat
     5941 caaatggaca aaacaaatca tcttcttcat ggattaaaat tttatttcct tccgattctc
     6001 ttgcatccaa tataggagtc attacttcat attcaacttt tataagtttc atagctttaa
     6061 tagcagccct ttcatcttca gcggctatta tagcaacttc atctcctaca tatctaacta
     6121 cgtcttcaag tattaattgg tcatgaggtg atgcttctgg atatgactct ccaaccattg
     6181 aatatctata atttggaaca tctttgtaag tgaatatgtt tactacacct tttaccttta
     6241 atgcattttt tgtatctatg ttttttatct tcgcaaaagc atgcggactt ctaagaagct
     6301 taattgttaa aacatcttta tgaaaaatta aatcttctgt atatattggt tttccagtaa
     6361 caatggcttc actatctatt tttcttacct ctttatttac catcacactc acctccaata
     6421 ctattataca ttctcactac taaggctttt gccatatctt ttctgtattc tttacttcct
     6481 cttatattac ttccaaatga aagttcttca cttgatactt caccagcttt ttctatatca
     6541 ttttctaaac ttaatatttc acttgcttta taggcaattt ttgctcttct tggtctagcg
     6601 cctatcgcaa ttttataaat acttccttct tttaacattg ctccatttaa tactgcaaaa
     6661 tcacctgtac attttcttat agaatcaaat acacctatag catctttttt aggtaatata
     6721 acttctatta atatatcttt ttcaagctca ctttctaaaa aatcagctaa gttcataacc
     6781 ccttttttat ataacttaac ctttgcatct accactagta atgaaggtat taaatctgaa
     6841 aatccatact tagcaaatac gctagctcct actctggctc cacttctaaa ttgaactcct
     6901 actatattag aaacagcgtt tgaaattacc ccattacagt aatttttaat aaccttattt
     6961 aattctaaag ttcttaaact tgtgtctgct ccaattaata tgctatcatc agtttcattt
     7021 atataatcta aagctaagtc ttttaaatct attgctgagc caatattttt actacctagt
     7081 tttaaaaatc cacatcctcc aagtattatg ttgttttttc ttgatattag cagttcataa
     7141 gcttcatcta aactttttgg aactacatat tcctttatag ttatcatact ctctccccat
     7201 atcaacttaa ttttcactta aaagtctaag tgtttaaata tttatgtatt gctcttaatt
     7261 gacctttata tcctgtacat ctacaaaggt taccatttac ataatctata acttcttctt
     7321 ctgttggatt ttgaagctct cttttcattg ccaaaaccat cattataaat ccaggagcac
     7381 aaaatccaca ctggtcagcc ccttcgtctg caagaaaatc tccaaacttc tttgcttcat
     7441 ccaagacccc ctcaagtgtt gtaatatgct gaccatctac tctagcagta agataattac
     7501 atgaaagcac ggccttgtca tttaccatca ctgtacataa cccacatgag cctgtatcac
     7561 accctttttt aacacttaca tatccatttt ctcttaatgt atctgctaaa aaatcatctg
     7621 gtcttatact taattctttt tctttattat ttatggtaca tttgataatc aaataaatca
     7681 cctcaaaact catattttta agctaaagtt ttactatact tttctatgaa taaattacat
     7741 ttattaataa aggctattcc atttatgacc ttccattcta ttttttatag ttcctcttac
     7801 acaaagtctc tcagctgtat cttgagcatc ttttaatgct ttctcttcat tagctgtcaa
     7861 tatttttgaa tcttccatta ttatatttcc atctactata acactctcta tatttttcat
     7921 actagaagaa tatactaatg tagataccgg attatgcata ggtatcgctt ttggtgaaag
     7981 cattggatta aatattagta agtctgcttt tttacctatc tccaatgaac ctatctcatc
     8041 ttccattcca atagctcttg ctccatctat agttgccaac tcaagaactt tttctgccga
     8101 cattgctaat gggtcacact tattaacttt atgttgaagt gctgttaatt tcataagttc
     8161 aagcatatct tgagagttat tactagcggc tccatctact cctaaactaa ctgttattcc
     8221 ttttttaagc atttctggca ctggtgcaac acctgaagat aagtacatat tacttgctgt
     8281 attatgagac actttcatat cgtatttttt agtaagttcc atatcttttt cagttagata
     8341 cacacaatgt accataagca catttggtcc aagtattcct aatttttcta aaacatctat
     8401 gtcatattgc ccatgcaatt cttttgctgc ttctctatca aaaggagtct ctgatatatg
     8461 tactgtaaat aaagcatcat catattcttt taccactctc catagcattt caagcatttc
     8521 ttgagaattt gaccatattg ctgcaggagc tactcctatt ttgattcttc cattttctgt
     8581 attatggtgt ttttcaaaca atcttcttac atctttttca accgtttcta catcttgcat
     8641 tattccagga tgaactccaa attgagctcc tgtattcata catcctcttc caagtattcc
     8701 tcttattcct aattctttat aagcatctat tattccatca caaagacctg gcttactatg
     8761 aggatacatg tagtcaacca tggtcgttat accacttcta agtccttcaa tacatccaag
     8821 cattgcagca tcataagtat cttttggctc taaatagtta gctgctggaa atgtcatagt
     8881 ttctaaccag tctttaagta ccatatcatc tcccaatcct tttaataaag tttggaataa
     8941 atgattatgt gtatttataa aacctgggaa taaaacttta cctttagcat ctataatttt
     9001 tttaacatca gtatatttac tttctatctc tttagaattt ccaatatcag caatcttgtt
     9061 atcttttact actaatgccc catcaaaaat aacttctctc tctttattca cagttactat
     9121 tatggcattc ttaataagta tatccatcat tcttatttcc ccccgcatta tttagtaatt
     9181 tttcttttaa tatattgacc tgttggatgt tctttaacat tacaactttg tactacatga
     9241 tttcctctca aaaatacatc ttcaacttta catttaacct taaatccttc atatggagta
     9301 taatctacat tttgtttttg agttttataa ctaattgttt cttctttatt gatatttaaa
     9361 atgactatat ctgcatcact tcctacagct atctcacctt ttttaggata cattccaaat
     9421 atttttgcag cattcgtaga agtaacttcc acaaacttat ttacacttat tctattttct
     9481 aagaccccat atgtatataa aagtgctaac ctatgttcaa caccaggtgc tccatttggt
     9541 atcttactaa aatcatcaat tcccaagtct ttttgcccct taaaattaaa tgaacagtgg
     9601 tctgtgccaa ctgtttgaat atctccacta gacaatcctt cccaaaggta atttatgtct
     9661 tctttttttc ttaaaggagg agacattaca tacttagcac cctcaaattt attcttatta
     9721 tataaattgt cttctaataa tagatactgt gggcatgttt ctactatcat atctatattt
     9781 ttttctctag ccatttttac agtttcaagt gactccctac aacttaagtg gactacatag
     9841 gattttgaat tagataattt agttatatca gcaagtcttg aaacagactc tttctcaact
     9901 atgttaggtc ttgtaagtgg atgatatttt ggctctaaat taccattttg tatattttct
     9961 tctatcaata catctagtaa atccccattt tcacaatgaa atccaactat acaacctaaa
    10021 tcccttgctt tctttaaaac cctgtatatt gttccatcat ctactttcat gccatcatat
    10081 gccatataca tcttaaaaga tactatccca tccgaaataa gtttttccat atgattaaat
    10141 gtatctttat ttaaacatgt aattgtcata tgaaaaccat aatcacaata acaatttcca
    10201 cttgcttttt tatgatatgc ttctacacct tgcagtaggt tttccccctc attggcttct
    10261 gcaaaatcta gtattgtagt agttcctcca gctatagctg ctcttgttcc tgtttcaaaa
    10321 ttatctgcag ttgtaataga acctgcattc atatcaaaat gagtatgtgt gtctattcca
    10381 ccaggaatta caattttacc tgtagcatca ataaccttat caccatttat ttctaagtta
    10441 tttcctattt caactatctt ttcatcttct atccttaaat cacttatata agtcttatca
    10501 gaggtaatta tagttccacc tcttagtatt gttcccatat atacctccta acttttaagc
    10561 tttaaagtat tttctaagta tcgtaaaact ttatttaaaa ttgtattttc taaaggtgct
    10621 atattcagca cctctagact atatttaaat aatttattgt gaactaaata ttattttccg
    10681 tatacagatt ttaatattcc atagtaacct tctgttccta taaataactg ttcttgctct
    10741 atatactcat ctattgtgtg agcaagattt tccttagaag gaccaaatcc tatagttttt
    10801 attccagctt cacctgcata gtgacttcca ttcgtacaga atgagtattg agttatttct
    10861 gaatctattc cagcttcttt taatccctta tatgctgctt gaacaaattc atcttcttcg
    10921 tcatataacc aacctgggaa gaatctttca ctttctatag tatttcctgt atagcaatca
    10981 gctttttcaa ccgcataaga aactttcgca tttaattctg ggtcttcttt catcatttca
    11041 tctaataaag cttgtattgg agctaatacg ccttctcttg tttctccaac taataatctt
    11101 ctatcaaaag tagctttgca ataatctggc actactgatg cccctggata tggagaagat
    11161 tttatatctg ttaaaactaa tattccatca cctaaaactg gatgagttgg aggtactaat
    11221 tgttgtatct tttggattac attagccatt ttataaactg catttacacc tttttctgga
    11281 ttagcagagt gagctggttt accaaaagtt tctactacta tttctcctct acctctctga
    11341 ccaatcttta agtttaactc tgaagactca cctattacaa catagtcagg ttgaacagcc
    11401 ttacttatct ctctagccga aacaccttca aatatttctt catgaacaac acctgctaca
    11461 tatagttctc cagcaaaatc tcgattagtg tctttagcaa aatacgctac agcagacatc
    11521 attgcagtat attgaccttt catgtcagaa gttcctctac catatatctt tccatcaact
    11581 atttttcctt caaaaggagg aacactccat ttacttgcat ctggaactgg aactgtatct
    11641 atatgtccat caaataatat tttcttacct ggcttgttac cttttataca tcctactata
    11701 tttccatatc tatctcttga ccaactatca aaacctaatt tttcaaaaga ctcttctatt
    11761 gcttttacaa caccttcttc atttccagaa gaactaggat tttgaattaa tttttggcat
    11821 atttcagtta cttcttgttt tctttgttcg tttaacattt cattatcccc ctagattttt
    11881 tataatgtat gtattttata tacttatata ttttctaaat tttagaactt atatatcttc
    11941 taaatatgta aaccaatatc taataattgc attttagtaa ttccttatat acttggatat
    12001 tctccatccc aaactatact tctatatttg tcagggtcag tatctccttc tgtgcttatt
    12061 aaaagtattc ttgaattttc atctaatttt aaatcttttc ttaaatctgc taaatcttct
    12121 ctttccatag ctgctactaa aagaccagtt gtaactgctc cagattctcc agaaattacc
    12181 ttttcatcac catttaaagg attacctaat gttctcatac ctttagcaga aacccagtct
    12241 ggtgctgaaa caaatgctgc agcatgattt tttaatactt caaatcctat agtatttggt
    12301 tctccacaag caagaccagc cattatagtt ggcatatctc ctccaacaaa tcttggtttt
    12361 ccatctcctg cttctgctga tttatagtag caatcagctt catcagcttc tactactact
    12421 gttattgggc attcatcgta tattgaagca acatatcctt gaactgctcc agcaagtgaa
    12481 ccaacccctg cttgcacaaa tacatgtgtt ggtctatcta ctccatattc ttttaattgc
    12541 tctatcgctt cttgagccat agttccatat ccttgcatta tccatgctgg aatttcttca
    12601 tatccatccc atgctgtatc ttgaaccatt actccatgat catcagcttc tgcataatca
    12661 ttagctaatc ttactgcatc atcatagttt aagtctgtta tactagcatc tgctccttct
    12721 cctttaattt tatttaatct aaactcagaa gaaccttttg gcattaatac aacagatttt
    12781 tgacctaatt tattagctgt ccaagcaact cctctaccgt gatttccatc tgtagctgta
    12841 aagaaagtta tttctcctaa tttttctttt atttctttcg aagttaattt gtcataccct
    12901 aactcagata tatctgtatc taatctttga gctaagtatc tacccataga ataagaacca
    12961 cctaatactt taaatgcatt taatccaaat ctataagatt catcttttac atatactcct
    13021 gcaacaccta atttttttgc taaaccttct aggtttgcta atggagtttc tttatattga
    13081 ggaaagcttt tgtgaaaatt tctaacctta gttatttctt cttcatttaa aaatttgata
    13141 caattttcct tatcaccctt tggcaaatta tttactttcc atttaatttc ttttaacatc
    13201 taaaatacct ccaaattatt gttaaatttt tatactttaa tatataagca attaacatgc
    13261 caacactaaa tatatactag atatttttct atgactcttt agtatagaaa ttgcaatgct
    13321 tttattattc tttcttttct atctttagtt catacaatgt atgtagaatt taataactaa
    13381 aaaacctttt ctatcttctc actttttaat tctcattttt aaaatttaaa aataaatctc
    13441 aaaacacttt gtttctatat tttttaatat aaaatttata aaatctaaat taaaacataa
    13501 aatagtgatg caataatcta ttacatcact atttgtatat atttaataat ataataaatc
    13561 tttagtggtc tctaagaggt gatttttcat cttttttaac atctaattct tcttttacga
    13621 cattccattt ttcaagacca aacttagaaa ccataactgc ctctgcagca ccttttccaa
    13681 gtctttttac tgtgtccata cctgtctcat cctcttgggc accttttgct tttccatctt
    13741 tactccatat tgtaccacct atatagcatc cacctaaacc tccagatact aacatttcat
    13801 gcattaggaa gaaatttatt ataggaagtt ttgtcaattc ttggccacca tatctagttc
    13861 caccagtagt tatagctgca ccaaccttat tttgaagttt tcctggatga actaaataca
    13921 ttggacgaag tctattaaat actgctgtaa gttgagcagt tatattcata tcataaactg
    13981 gagaacctac tataattcca tctgcctcca atattttttg ctctaattct tggatatcat
    14041 cttttataat acacatagtt tcttttctta tacatgcatc acaatgtaca caaggactta
    14101 tttttttccc tctaactgac caatactcag tttctatacc aggtatagtt tctgcttctt
    14161 tcaaagcttc taacactgca tattctgttg ctcctttacg tggacttcca caaataccta
    14221 atatctttgc cataataata tctcctcacc aaataaatct atttttatta tctatttaca
    14281 aatacaataa attgttgttt gtgctttaat ttctactatt ttaattttat tggcaatgaa
    14341 tgtatttcta catcagttgc attatatact gcatttgcaa cagctggcgc aactggtaca
    14401 acagcacatt ctccaatact cttaccacca aaaggtccag ctttttcata actatttaca
    14461 aatgcaactt gacattttgg catatctaca gttctaaata atttatatgt tttaaaatca
    14521 gtagctttag gtctaccttg ttcatcaaat gccaattctt cactcaatgc gtatcctaga
    14581 cccatatgaa tcccaccttc taattgacct tctaaattta aaggatttat aactctacca
    14641 acatcatgta cagctacaaa atcaactact tttatatcct tagtttcttt atctactaaa
    14701 acttcagcaa aatgtacacc ataagaagta agtccagctg gtgaagaata atcttcaaca
    14761 acagttaact ctcttaatga tttactttga ctatatactg cgacatcaga aagtgatgct
    14821 tttacatttt catcaagttt actaattaca tatccattgt ctagatataa atcttctttt
    14881 gatgtttcaa gtaatttttc tgcttcttct actataaggt ttttcaattt ctctgctgtc
    14941 tttttagctc ctccaccttc tacaaatacc cctctacttg catagtcacc aaggttgaat
    15001 gtacatgcat ccgtatcagt ttcaaatgta tcaactttat caggtgttat tcctaaaact
    15061 tcagctacca tcattgtttg catagttacg acaccattac ccatatcatg tgctcctgtt
    15121 agtagtgtca atgtaccatc ttcatttaat ttaagagtca atgtaacaac atctctatgt
    15181 gcaccaaaaa ctccattacc atgtgcacct attgccatac ctatacctct taaatatttt
    15241 ccttcttctt ttggaagttt tttcttttca tcccacttaa acatcttctt accttgctct
    15301 atacaatcca atactctagg atttccaaga gaaccgcgga agattatatc gacatcatct
    15361 ggctctactg catttttatt ttgaaaatcg actaaatcca tacctatttc cttggctatt
    15421 ttcgcaaatt gagcctgttg agccataaat atttgaggtg acccatagcc tctcatagct
    15481 cctgctattg gtgtatttgt atatacaggc atcccagtaa actttatatt tggtatttta
    15541 taaactttaa atactttatg actaagtgct cctataacat ttaaagcact tgatgcatat
    15601 gctcctgtat ttgtatatat ttttatatct tgtgctatta cttcaccatc tttatttaca
    15661 ccagttttta attttacaaa agacccatga cgagttcttg ttgatatcat agattccttt
    15721 cttgtaagaa ccattttaac tggtcttctt gtcatttttg ataatgcggc tgctactggt
    15781 tcaattgtaa gttctaattt tccaccaaat gagcctccaa tagcaggtct tataactctt
    15841 actttattta atgacatccc aaaaatttga gatagtatta ttctaaatcc aaatgtattt
    15901 tggcaaggtg tatgtacagt taatttatca ttataatcat agtgtgcaat tacactatga
    15961 gtctctattg cactatgatg tattgctgga gtagagtatt catcttcaaa tatatagtca
    16021 cattccttga agcctttttc tacatctccc ccatttatgt tgttttgaga aactatattt
    16081 gaatctccat gtatgcagat agcatcttct tttattgcat cttgtatagt aaatacagct
    16141 ggcaattctt catattcaac ttttataagt tttacagctt tctctgctat ttctggagtc
    16201 tctgcagcta ctgctgcaac acggtcacca acaaatctaa ctgtatctga aaatataact
    16261 tcattttcag gtatattgtg ctcataaaat cttaatgaac cattgtattt tctttgtgat
    16321 gtattgagat aagtagctat agctctaaca cctactactt tctcagcttc acttgtatct
    16381 atacttttta tttttgcatg tgctacagga cttaaaagca tcttagcgta aagtacattt
    16441 tgtggtttca tatcagccac gtatttcatt tgaccagtaa cttttaaagc tgcatctttt
    16501 agagggtatg attttcctat taccttttcc ataattatct accccctaat ctagctgcag
    16561 ataattttac agcttctata atctttacat atcctgtgca tctacagaga ttttctttaa
    16621 aagcttcttt aatttcatct tcattaggag atgcatttct atttaaaaga gcttgtgttg
    16681 ttataatcat accaggagta caatatccac attgtacagc tccaacttct ataaaagctt
    16741 cttgtattgg atgaagattt tctccattag caaaactttc tatagtgtat ataactttac
    16801 cttcgcagtt ttttgcaaga agtttacaag atttcatgtc aactccatct atcaaaactt
    16861 tacatgctcc acaactgcca ctattacaac cttgtttcgc tccagttaga tttaattttt
    16921 cacgaagtac atgcaaaaga gtttcattct tttcaacttc tatttcatac atgtaatcat
    16981 taatatttaa ttttattttt tccattttct cctcctacag ataaactaat gttgtaatag
    17041 aatattatta caatcttatt ttaatattag tataagctat tccacttatg tccttccatt
    17101 ctattagtta cattagctct cttacataat tcttctgctg caagttgtgc attaagcaac
    17161 acttgttctt cactttcaat tgtagttact tttccatttt caagtattac atttccatca
    17221 actaaaacac tctctacatt gtgcatacta gctgagtata ccaaagttga tacaggattg
    17281 tgaagaggta ttgattttgg agaaagcatt gagttaaata ttactaggtc agcttttttc
    17341 ccaatctcca aagaacctat ttcatcttcc attctaagag ttcttgctcc ctcaattgta
    17401 gccatttcaa gaactttctc tgcagaaatt gccagagggt cacaattatt tactttgtgt
    17461 tgtaaagctg ttaatttcat aagctctacc atatcttgtg cgttattact tgctgcaccg
    17521 tcaagaccta aactcacatt tatacctttt tttaacattt ctggcacagg tgcaacacca
    17581 gaagcaagat acatattact tgctacatta tgagaaactt ttatgtcata ctttttaagc
    17641 atttccatgt cttcatcagt tatacataca caatgtactg ctacaacttc tggacctact
    17701 ataccccatt tttcaagtaa ttttatatct atttctccat gtatatcctt tgttgctgtt
    17761 cttgcaaatt ctgtttcaga tatatgaact gtaaaatatg aatcatattc tttaactata
    17821 ttccaaagca tcttacccat ttcttcagat atagcccaca ttgaagaagg agctacacag
    17881 atttttattc ttccattttc tgaattatga tatttttcga atacccttct caaatctttt
    17941 tcaaccgttt ccacatcttc tataagctct ttgtgaatac ctaaatctat acaacctcta
    18001 cctacaaccc ctcttatacc taaatcttta tatgccttta ctattccatc tgtaagtcct
    18061 ggtctattgt gagtatgcat ataatcaacc attgtagtta taccactttt taaaccttcc
    18121 atacatccaa gcattgcagc atcataagta tcttgttctg ttaagaattt tgctgatgga
    18181 aacatcattg tatttaacca ctcatgaagt gccatatcat cacctaaacc ttttaataac
    18241 acttggaaca ggtgattatg agtatttata aagccaggga atattacttt tccattagca
    18301 tctataactt ttttagcatc aagatactta ggctcaatat caactgtatt tcctatatcc
    18361 aagattctgt caccttgaat agctattgct ccatcaaata taacatctct gtctttatta
    18421 actgtaacaa ttattgcatt ttttattagt atatcaatca tcttaaatcc ccctttgaat
    18481 actttcatta caacatttta ccatatggtt ttctcatatt atatctaata catttatcca
    18541 atattttcct caagatttga agtaacgttt tcagcacttt tacgtgactt taatttaaat
    18601 aatgtttgaa gtaatatagc tattattgct ccagaaacta tacttgaccc tccaaggaca
    18661 gttccaattg tttctgggaa ttggtctaaa acttgtggag cagttgtaag acccatactt
    18721 gttgctatag atacacctgc tataagattg ttttcttcag tgaatccatc catactcata
    18781 atagagaaac cagacattgc tattgatgag aaaactacta aagttgctcc accaacaaca
    18841 caagatggta tagttgtcat tattgcaccc aatgctggag atatccctgc tagtaataat
    18901 acaagtgccc ctagtgctat tacaaatcta cttgtaactt tgttcataga tacaattact
    18961 gtattttggc taaatactcc agttggaaga gctgcaaata gtgatgaaat tatagaagtt
    19021 atagaatttc ctaaaacaac tgatgctaat tcttcgtcag ttacctctct atccattgct
    19081 cctactgtag ctattgtaca ggcacccatc atatcagcta tacctattat gtaaataata
    19141 gtaaacatta ctattaactc tggtctaaat tctaatccaa aagctgctgg tgttggtaaa
    19201 gatactatag ctgctccttg aacggctgag aaatcaacca atcctaaaac taaagatata
    19261 gcataaccta ctaatattcc tattaatatt gaagaatttt ttactaaacc tttaccgtat
    19321 ttatttatca ttattattac aaaagcaact ataactccta ctgtgaagtt tattgggctt
    19381 ccaaatgttt gtgtgccttc tcctccagct aaattcttta tagctgttgc gaatagtcca
    19441 agtcccatac atgctattat agttccagaa actattggag tgaatatatg tctaattttc
    19501 ttaactccaa atcctacaaa gaatacgatt accccagcag caatctgtgc tccaaataga
    19561 gctgctaaac catctttagc tgctactgaa agacaagctc ctaaaaatac ataactcatt
    19621 cccatcatta caggtaatct tgaacctaac ttaaatcctt taaataaagg tattggatat
    19681 aactgtaaaa acgttgctaa acctgctgct aacatagaac cctgaatcaa cattgttgct
    19741 tgcccctggt taagtcctac cacgtttgct attaatatcg caggtaccat atttcctact
    19801 atcattgcta acagatgttg taaggccaaa ggtattgatt cctttagtgg tggtatccca
    19861 tcgacatcat attttgaagt attacgtgtt ttcataaaat aaatcctccc atacataaat
    19921 atcttgacta aatattttta taattatttt ctataaatat ttttatgacc atgaatttat
    19981 tgctcaaccg ataaacacag ctaaactgag ctatttttat aaaaaattaa caaatttcta
    20041 aatattccct ccccgatttt caacttaaat gaaacattaa ttgcctggta tatatttatt
    20101 tgttaaattt tttattaaaa tgaatagcta tcaaaaataa tctaacaatc tcatactaaa
    20161 taatttaaac aattctttta aattattata tcttaattag tctcttccat aacagctctt
    20221 tctttagcct catcctctag acttttattt ggtattatta gatttaaaac gaatgcaacc
    20281 aatgttgcaa ttactattgg agattctcca aatactaact ttacaaatga aggaaatgcc
    20341 tcttgggctg ctggtactga tgttattccc atgcctaagg caattgccaa accaacaatg
    20401 gtaatatttc ttgatgacaa ctcttctgta ataattaact ttatacctgt cattgttatc
    20461 atactaaata tagatattgt agctcctcct aaaacagaat atggtatagt agtcattaat
    20521 gcaccaaatt taggcacaaa ccccgacaat aacataaata taccagctat tccaagcaca
    20581 aatctactta ttactttgtt catagctacc atccccacgt tttggctata tgatgatggt
    20641 ggtaaacctc caaacaaagc tcctatcata gtacaaattc cacttcctat tactccactt
    20701 gataattcct tatctgtgat attcctatcc atgcctccca ttgtagtagc tgataaatct
    20761 ccaattgctt gaacagcatt gattatactt acaattacta ctgagattat tacaggcata
    20821 tcaaattcca tcccaaattc aagcggttta ggtatcgcaa accaagttgc ctctctaata
    20881 gggtcaaaat ttattatacc cagaactaaa gatacacaat atccaactat aattcccaaa
    20941 aatattgctg ccaattttgc atatcctttt gcaaattgac taagacctat aactaccact
    21001 aatgttatag ccgctactgc ccagttttgc atagaggcat aagtaggact atttacacca
    21061 cctgccatat aatttattgc tataggatac aaagataatc caattgtaaa aaccactgtt
    21121 cctgcaacta tattcggaaa aaattttctt atctttttta tgaatatccc tattaatact
    21181 gtagccactc cccctattaa ttgtgcaccc aaaatgcttg ctaatccata atttcctccg
    21241 actgctacaa gtgttggtac ataagtaaaa ccaactccaa aaattatagg caatcttgaa
    21301 ccaatatttc ctattggata tagttgtata agtgttgata taccagctat tattaatgca
    21361 tattgcacta aaagtgttat ttgtgtaggg ctaacaccca cagctcctcc tacgactata
    21421 ggtactgtaa cagttccaac tatcattgct agtatatgtt gcattgatag tggcaaacac
    21481 tttaaaattg gtggccttcc atcaatatca aacatagaaa cattcttttg atatttactc
    21541 aaggttatca ctcccaacta ttttttaatg acagttatta ttatctcaca caacatcctt
    21601 tcttttttta ttttgtcatc tcaatttaat atgaagcaat tataatgcca attttttaac
    21661 ataattgttt tacatatata atttttatta tatttgttag tatttgataa tttattaaag
    21721 catttatttt tttaaattct tttaaatttt ttttattttt ggataataaa atgctattac
    21781 acaaaaaaac acttagaaaa tagttttcca agtgtttttt atcaatttta taatttcaaa
    21841 ttttagaatt gagaataaaa aacctaatta ttatacatct atttttctgt acaatgtagc
    21901 tagtgatatt ccaagttttt tggcagccag ttttttagac ttagtatcat ttccgtaaaa
    21961 ctttaacact ttctttatat aggctctttc aaactcttca agtgtaagta tgtcttgttc
    22021 catacttatc tcatagtcac tttcctcaag taactttata tttatattat ttaacttaaa
    22081 atattcaagt atctttctat ttattattcc tttatgtatt attccatctt cctcaagaag
    22141 gtttaatgcc agttctatag tattttccaa ctctctaata tttcctggcc aaggatactt
    22201 aagcaacatt tgttccacat ctggttctat atggtacacg tttttcttca tttttctcga
    22261 atatttgtct ataaaattat ttattaatat ttgtatatcg tctcctcttt ctctaagtgg
    22321 aggcacttct aaaggtatta cattcaatct atagtataag tcttctctaa atttattttc
    22381 cttaaccaac tcaaataaat ctctattagt agctgatatg attctaacat ctatatcaat
    22441 tagttcattt gaacctattc tcgtaaactt cttttcctgt aataccctca ataactttga
    22501 ttgtagttga attggcatat ctcctatctc atctaaaaat acaacccctc cattggccaa
    22561 ttcaaactta ccaattttac cttttgaatc tgctccacta aaagctcctt ttgtatatcc
    22621 aaaaagttca ctttctagta aaggttctgg tatagcagca cagtttatag caacgaatgt
    22681 tttattccct ctgtttcctg cgagatgaat ggctcttgct attaattctt ttccagtgcc
    22741 actttctcca gttatagcta cagtagaatc actatttcca attttgcaaa ttctattctt
    22801 aagagcacgc attacatcag actctccaat tatggagtct actgttgttg ttccccaaat
    22861 attatatact tccattccac catacattac tgaatcactg tgtttgtcca taaaatatca
    22921 actccaaata agtattctat ataataatca ttcacattat ctatttaatt atatcataac
    22981 agaaaagctt agttataatt taataatatt attttaaaac aaaacttaat aaaaagtata
    23041 tctacttagt tttaatttga tagatatact ttcttcattt tacaaaaatc ctctattaaa
    23101 taagcaatta caatgccata ttttatatga aaataatttt taaaattttt aatttaagtt
    23161 cttatta
//
LOCUS       DAEPOZ010000032        22963 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.032, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000032 DAEPOZ010000000
VERSION     DAEPOZ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22963
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.032"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(99..1196)
                     /locus_tag="KR440_001042"
     CDS             complement(99..1196)
                     /locus_tag="KR440_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBH0698101.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILFTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRN
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
     gene            1489..3015
                     /gene="glpK"
                     /locus_tag="KR440_001043"
     CDS             1489..3015
                     /gene="glpK"
                     /locus_tag="KR440_001043"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBH0698102.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFESGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKEIKMLRVDGGITKNNFTMQFQSDILDIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTKQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            3056..4171
                     /locus_tag="KR440_001044"
     CDS             3056..4171
                     /locus_tag="KR440_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBH0698103.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLVFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(4452..5216)
                     /locus_tag="KR440_001045"
     CDS             complement(4452..5216)
                     /locus_tag="KR440_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBH0698104.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            5475..5565
                     /locus_tag="KR440_001046"
     tRNA            5475..5565
                     /locus_tag="KR440_001046"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5509..5511,aa:Ser,seq:gct)
     gene            5653..6441
                     /locus_tag="KR440_001047"
     CDS             5653..6441
                     /locus_tag="KR440_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HBH0698105.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHIGVLFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSVDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEISE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            6462..7202
                     /locus_tag="KR440_001048"
     CDS             6462..7202
                     /locus_tag="KR440_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBH0698106.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDSLQNAKFKKVGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAVVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            7380..7754
                     /locus_tag="KR440_001049"
     CDS             7380..7754
                     /locus_tag="KR440_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698107.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKDNKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            7846..9306
                     /locus_tag="KR440_001050"
     CDS             7846..9306
                     /locus_tag="KR440_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBH0698108.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTIGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIEEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLNVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFETWFESFKAELENAIKK"
     gene            9359..12235
                     /locus_tag="KR440_001051"
     CDS             9359..12235
                     /locus_tag="KR440_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0698109.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKILNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILNDKNINDKPIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQEGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRIEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKDKKLKWIRMNIFEDELKVLG
                     VVVDVTKEFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLD
                     NLKYINDTYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRD
                     IINSVKKQMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTI
                     KGEVSEFDINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPK
                     IKTFSSPIDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLS
                     KVDFEEIEDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNG
                     DAVLLYIMPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVISEGVETKGEME
                     TLINLGVDYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            12312..14378
                     /locus_tag="KR440_001052"
     CDS             12312..14378
                     /locus_tag="KR440_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBH0698110.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVYTANRLIDKNKNRYEKVIKCSQGDGGSVTYISPHDSEEEAL
                     YIAKEIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     misc_binding    14970..15180
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15406..16221
                     /locus_tag="KR440_001053"
     CDS             15406..16221
                     /locus_tag="KR440_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBH0698111.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     gene            16249..16926
                     /locus_tag="KR440_001054"
     CDS             16249..16926
                     /locus_tag="KR440_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBH0698112.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            16985..17707
                     /locus_tag="KR440_001055"
     CDS             16985..17707
                     /locus_tag="KR440_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBH0698113.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
     gene            18355..19506
                     /locus_tag="KR440_001056"
     CDS             18355..19506
                     /locus_tag="KR440_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBH0698114.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDVVISKVNNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            19552..20727
                     /gene="thiI"
                     /locus_tag="KR440_001057"
     CDS             19552..20727
                     /gene="thiI"
                     /locus_tag="KR440_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBH0698115.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            20883..21686
                     /locus_tag="KR440_001058"
     CDS             20883..21686
                     /locus_tag="KR440_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBH0698116.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKDKNEVIKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGIEMARDIHQSMKDLIASFAQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYNPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            21929..22570
                     /locus_tag="KR440_001059"
     CDS             21929..22570
                     /locus_tag="KR440_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HBH0698117.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
BASE COUNT         9062 a         2370 c         4151 g         7380 t
ORIGIN      
        1 tatacaaaaa tataaaatat aatttattat aggaaaattt aaaaaaatca gtctatttaa
       61 tttatactga tgtattgtag gattttacaa acaaactgtt atataacttt ttttatctcc
      121 aattttgatt ttaagatatc taagaattta taacaatctt ctggatatat ttctcccata
      181 tttgcaactt ggaatatgtt agcatctagg aatttgcctt ttcctggata gacaacatag
      241 ccactttccc tttctaggcc taatataaac tcatttacat tgatttcttt aggtagaaat
      301 acagatgtta ctgtattaga catatactta tctggaagta aaaatgttag ccccaattct
      361 tccatgccat tccttaaaat agcagaacat tctttgtatc tttctatttt actgtcatct
      421 tccaaaaact cagttaaagc ttcattaagg gcaaatatta aagtaacatt aggtgtattt
      481 ggtgtttgag aagatgattt agcaacttta tagtgtttat aaagattaag atatacattc
      541 ttacattgat tttctttaat attttgtaaa atactctctt tagcacaaag gtatgcagaa
      601 cctggaaatg ctccaagagc tttaccacca acacctgtta ttatatctat attaaactct
      661 tctacatcaa tatattcacc tgctgaggca cttattgcat ctacaaaaaa tattttgtta
      721 tacttcttag caagttttcc tagttcaggg actggattta tcattcctga actagtttca
      781 tggaacacca tagcaattac ttttatatca gaattttcaa ttatatattt ttctaaaatg
      841 tccaggtctg ggtattctgc ccattcaaaa ttaggcttat atgtttttac attataagta
      901 aataatattt cttctagcct ttctccaaaa acaccattac taactagcag aactgcatct
      961 tctttgtcaa agatagaaga tagtacacat tcatttacag atgttccaga gccagataca
     1021 attaatgagt agtaattaga agttgcatta tatagtttat tgattttttg ttgtgtatct
     1081 ttgaataaat ccataaactc gtttcctcta tggcatatat catagtgaag aagtgaattt
     1141 ctgactttat ctgtaaccat tactggtcca ggactgaata gatatgtttt tttcattgta
     1201 aagcctcctt aaaaagttaa atttaaattt tcgaatgact gactatgtgt aaatattaaa
     1261 atattgtgtg ttattttaac tagattatag aataaaataa tgaatattgc aataatattt
     1321 caataaaaat acttttttaa tcaccataat taaaaataac aaaaaatatt gtaatgaata
     1381 ttgcataaat ccacatgatg atataaaata taaataactt gaatagatta tatattcaaa
     1441 aaataaaaaa gaatttatta attctgaaaa taaaggggga cattttaagt gaaatatgta
     1501 ttagcaattg accaaggaac aacaggaaca agagcagtat tgataaacga aaaatcagag
     1561 tttgttttta gcgattatat ggaacatact caaatatatc cacaatctgg atatgttgaa
     1621 catgacccta tagaaatttg gaataataca aaaactgtga gtaataatgt attacaacag
     1681 gcttttgaaa gtggaatcaa tgaaagtgat ataaaaggaa ttggtataga taatcaaggt
     1741 gaaacagtaa tgttatggga taaaaataca ggtaaaccac tttacaatgc tatcgtatgg
     1801 cagtgtagga gaacttatga ttatgtggaa aatttaaaga atataccagg tttagaaaat
     1861 aagataatag ataagactgg gttaattata gacccatatt tctcaggtac aaaaattaaa
     1921 tgggtaatag ataatgtaaa aggtgtaaaa gaaaaaatta aaaatggaga agtacttgca
     1981 ggaacattgg atagttggtt aatatggaaa atgacaggag gaaaatcttt tttaactgat
     2041 gtatctacag cagcaagaac aatgcttttt aatataagag atatggattg ggatagagag
     2101 ttgcttgaca ttataggagt gcctagaagt attttagcag atataatgcc tactagtggt
     2161 gattttggaa ctacagataa ggaggcattt tgtggggttt ctataaaaat tactgcttca
     2221 atggtagacc aaccagcagc tttatttggc aatggatgtt ttgaaccagg aatggttaaa
     2281 aatacttatg gtacaggatg tttcttatat atgaatatag gtgaggagtt aaatatagca
     2341 ggtaatggta ttttgacaac agttgcttgg aagataggag ataaagttac atatgcttat
     2401 gatggtggag tgtatatagc aggtgctgca atacaatggc ttagagatgg aattggagtt
     2461 ataaaaaatt attcagaaac tgatgacatg gcaaattcca tatcaagtac tggaggggta
     2521 tattttgtac cagcatttgc aggtatagca gcaccttatt gggaccaata tgctagaggc
     2581 acaatggttg ggataacagg aagtactaaa aaagaacata tagtaagggc tactcttgag
     2641 tctgttgcac ttcaagtcaa ggatgttgtt gattccatga atttggcttc agggaaagaa
     2701 ataaaaatgt tgagagtaga tggtggaatt actaaaaata actttacaat gcaatttcaa
     2761 tctgacatat tagatatacc aattgaaatt gcagaaaatc ctgaaacgac agtacttggt
     2821 gtagggtata tggcagggct ttcctgtggt gtttggaaca gtattgatga aattagaagt
     2881 gaatttacaa taagcaaaaa atatattcca aatatgacta agcaaaaaag agaagaaata
     2941 ttacttggat ggaatcaagc agtaaaaaga gctatgaatt gggaaaacga taccaaaaag
     3001 gtgtgtgcta attaagttac aatagagtat tttacatgta taggaggaat taaaaatgcc
     3061 ctttgataaa attatattat acataatggc tgtgggagtg cttttaggag cgttagatag
     3121 aataattgga aataaatttg gacttggaga gcagtttgaa gaagggttta attcaatggg
     3181 tccattggct ttaggaatgg ttggtatagt ttgtttagct ccagtaattt cagatgtact
     3241 aggtcctata ataataccaa tattcaatct ctgtggagct gacccatcta tgtttgcaac
     3301 tattttagcg aatgatatgg gagggtatcc attggctatg gaacttgcac acaataaaga
     3361 ggcaggaatg cttgcaggat tagtcgtgtc atccatgctt ggatgtacat tagtattttc
     3421 aattccagta ggactaggtc ttatagaata tgaagacaga ccattctttg caaaagggtt
     3481 actgataggc ttgataacaa taccttttgg aggtattata ggaggattaa tagcaggatt
     3541 taacttatca atggtaatta taaatatgat accagtgata atattggcag taatattggc
     3601 agtaggttta aaatttaatt ctacacttat gataaatggg tcattagttt ttggaaaatt
     3661 tatatctata ttgataacga taggtttggg agcagcagca tttcaagaaa taacaggtat
     3721 agtacttata aaaggcatga cacctataag agaaggtatt caagtaattg gaagtattgc
     3781 aatagtgtta ttaggtactt ttccaatatt acatttatta gttaaagttc ttaataaacc
     3841 acttacaata gtgggtggaa aattaggtat ggatgccact agtgcagcag gattagtttt
     3901 tactttagcc aatagtattc cagtatataa aatgatgaaa gatatgagtc caaaggggaa
     3961 agtagtaaat actgcttggc tggtttgtgc tacagcagct ttgggggacc atttaggatt
     4021 tacagcagga gtagttccag agatgattac acctgttgta attggaaaat tggttggagg
     4081 agtattggca attgcacttg ctatacttat gacaaaagat ttaactttgg aagaagaaca
     4141 aagtattaaa atgaagaaag atgcagtgta gaataaacaa tacttaagtc aaatttaagt
     4201 tattttaaag taggagttta aataactaaa aataaattaa aaagacagac aattaaaaaa
     4261 taatcgaata accaaaactg ataaattaaa taaaatggca atcaaataac taaaaatgat
     4321 aagctaactg aaagtagtta cataattaaa atataaagta tagtttagac ataagtggag
     4381 ctaaataaat aattatttgg ctccttaatt ttaatttatt acatcaagga actaaaaatc
     4441 aatcatacaa gctaattatc agctatgact aaaggtattc ctttttgaga aaactctatt
     4501 attatattat ggtctatact agcagaggta ataatagtat ctatgtgttc tataggagct
     4561 actgatgcaa atgaatttat accaaactta ggaggttcga caactactat agattttcta
     4621 gacctttcaa tcattttttt tctaaccagt gcttcttcta agtgatagtc agagattcca
     4681 ttttcaagag tgatacctcc agcacctaaa atagctttat caacattaaa ttgttcaata
     4741 gctgagagtg taagtggacc agaaaaagaa ccttcagttt ctctaagata gccaccaagc
     4801 acatatactt ttatattagt attcataagt tcatttgcaa ctaaaattga gttggttatt
     4861 acagttagat tctttttatc tttcaaattt tttgaaactt caagtacagt agttccagta
     4921 tccataaaaa tagtatcatt tgtatttata aactcactac atttaatagc aatctgtttt
     4981 ttatcgtttg agtttatttt ttttctttta acatagttaa gttctacgct acttgctata
     5041 actggtatag caccaccata aactcttttt aaaagaccga tactttccat atgggctaaa
     5101 tctcttcgtg cagtttctat tgatatatca aacatttgaa ctatgtcatt tattttaatt
     5161 atattatctg atttgagaat acttaaaatc ttctcgtatc tttcctgtgg taacataaaa
     5221 tatcctcctt ggactagtat tattttagtt ttggcataac ttacaattaa gtcagagttt
     5281 aggtatattt gttaatgtat gtatatttat caaataatat tataagtgtt atgcaagata
     5341 attttaccat atttaaaact tacatttaag caaaaaagta aaaatagatg tttaataatt
     5401 caaaataact caaattttat aaaacaattg actaaaatat gattttatta tataatttac
     5461 tagtatagag atatggagaa gtactcaagt ggctcaagag gatcccctgc taagggatta
     5521 ggctgggtaa ccggtgcgag ggttcgaatc cctccttctc cgccaattgt taaaggctat
     5581 ttttattaaa gatagtcttt ttataatgtc ataaatgatt agatatgtat aattaaagaa
     5641 aggagggaat agatgacaac tttaattttt ttcatagtgg ggatagcatc tttggtagtt
     5701 gcttttgtta ttgatggggg acatattgga gtattatttg tattttcatc agcacttgtt
     5761 gtgtttggtg gaactatagg cgctgttgga gcttcgactc cattccctgt gtttaaaaag
     5821 tctataaaga taatgttatt agcttttaaa aataaaaagc ctaataacat agaaaatata
     5881 atctatttca aagaaattgc aacaaaggct aggaaagagg ggcttcttag tgttgacaaa
     5941 gaaataagtg agaatgatga attagaccct tttattagaa aagggcttga acttatggta
     6001 gatggggtgg atgcagttac tataagaaat atattggaat cacagataca gataacatca
     6061 aaacgacata aagaaggaat agcaattttt gattcagcag gtggatatgc acctacactg
     6121 gggataatag gtacagttat gggattagta catattttag gtaatctttc aagtaatcca
     6181 gatgcattag gacctcaaat ttctgttgca tttttggcta ctttgtatgg tgttggttct
     6241 gccaatttaa tatggttacc aatagcatca aaacttaagg ctataaatca acaggaaata
     6301 agtgaaagac ttttggtgat agaagcaata ttatccatac ttgagggtga taattctaat
     6361 ttaatgacag aaaaattaaa aatattttta aattcaaaag aactaattga acttgaagaa
     6421 attggtggta gagctgaata atataaaagg atggaatgtt tatgttgtat gatgaagatg
     6481 aaaaagaaga aaacaatgag cgatggcttt taacttattc agacctcata acattactta
     6541 tgattttctt tgtaataatg tattctatga gtaatgttga tgcggagaaa tataaacaat
     6601 tatctcaatc tctaaattct gcttttggtg gttcttctgg agttattgaa ggtggtaata
     6661 gcaaaataga accagtagtt gaaccaggaa gtaatgattt agatagttta caaaatgcta
     6721 agtttaaaaa agttggtgaa gaaatccaga agtatttaaa tgaaaatggt atggctaatt
     6781 cagtatcatt aagagttcaa gatagaggat tagtaataag ccttaaagat acaatactat
     6841 ttgatacagg aaaagcagtt gtaaaggata attctagaga taagattatt caaataggta
     6901 agatgttaaa tgaaatgaat agttacatga gagtagaagg tcatacagac aatatgtcta
     6961 taaaaaattc agagtttaag tcaaattggg atttatcagt tatgagagca accaatgttg
     7021 ttcaacttct tattgacaat gcaggtatag caccagataa attatctgct gtaggatatg
     7081 gtgagtttag accaatagca gaaaattcat ctgaagaagg taggtcaaaa aatagaagag
     7141 ttgatatagt tttagttgat agtaaatatg ataatgtaga aaatgtttca aaggataaat
     7201 agttaaatta gctgtctcaa tacagaatta attctaaagg agatggcttt tttatgcgaa
     7261 aattaaaaat agatacatag taagttacct ttttgtagga atatatggta tcttaaatat
     7321 ataatattta aataatatat aagctcaatt aaaaaaattt tatagaggat gggaaataaa
     7381 tggggaataa aaataaaaat acgaaatata aagataaaac aagagaaaga tataataaaa
     7441 aagttgagaa tttaaataaa gaaaaattaa ataaagataa taaacagtct aagaaggtag
     7501 ataagattga tgatattatt aataaatcta gtattgtatt atgtgtactt ctagtggtgt
     7561 tttcattact ttacttattt aaattggttc aacttaatat aataatgata cttttagtaa
     7621 ttacagtagc tattcaacaa ggattggtgt catttggtgc atttagaaat aaaaattatg
     7681 taaaagcttt attttctata tttgtaatag tatgtttctt aattgctttt aaatatgttg
     7741 taaatcctgt ttaaatatat caataaacgt tctataatta atataaatag tgaagttaca
     7801 gttttaatgc tattataaat tttataattt gaaggagtag attttatgaa taggtgtaag
     7861 tttgcaaaat taattatcac atcaatgatt gttttattac ttacaggatg tagtttaaaa
     7921 aaggaagata attataagtt agacccagaa aatccgacat caatacaaat ttggcattat
     7981 tataatggac agcaaaaaat ggcttttgat aagcttgtta aggaatttaa tgacactata
     8041 ggtgctgaaa aaggaattat tgttgaagcg tttagtcaag gtaatgtagt tgatttatca
     8101 gagaaagttt tagattctgc aaataaaaaa gttggctctg aagatgtgcc aaatatattt
     8161 gcagcatact cagatactgc atatgaaatg gataaattag ggttactagt agatatggat
     8221 aagtacataa gtaaagaaga attaaatgaa tacatagacg aatatgtaga agaaggcaga
     8281 ataaattcta aggatgagtt taaacttttc ccaattgcaa aatcaactga aattttgatg
     8341 ttaaataaaa ctgattggga taaatttgca aaagctacaa attcaaatat aaatgatata
     8401 aaaacaattg aaggattagt agagacatca aaaaaatatt atgaatggac agatagttta
     8461 acgaaagagc ctaatgatgg taaagcattt tttgggagag atgctttagc aaattatatt
     8521 attataggaa gtaaacaact aggagtagaa attttttctg ttaaagatgg taaagttaca
     8581 ctaaatattg aagaaaaagt tatgaaaaag ctatgggata atttctatgt accatatata
     8641 aatggatatt ttgcttctta tggtaagttt cgttctgatg atgcaaaaac aggtgatata
     8701 ttagctcttg taggctctag ttcaggagca gcatactttc cagatagagt aatgctaaat
     8761 gataatgaga gttataatat tgaaacatct gtttttcaag caccacgttt taaaggagaa
     8821 ggaaaatatg ctgtacaaca aggtgcagga atggctgtaa caaaatctac taaagaagaa
     8881 gaatatgcat cagtagaatt tttaaaatgg tttacagata aagagcctaa tataaaattc
     8941 tctattgctt ctggatattt accagtaaag aaaaaaacga atgatttgaa tgttataaaa
     9001 gaagttatga agaaagataa tttgagttta tctgataaag ttgaaggttc aatagcctca
     9061 tctattgaac aaattaacag ttgtaattta tacactaata aagcatttga tggtggaact
     9121 aaagctagac aaatacttga atattcaatg cttgataaag ctaaagaaga ttcaaataaa
     9181 ataaatgaat tggtaaaaag tggaacttct aagaaggatg cggtagcaca atttaataat
     9241 gataagaatt ttgaaacttg gtttgaaagt tttaaggctg agcttgaaaa tgctataaaa
     9301 aaatagatga attggcagtt agtgtgtaga caagcacaaa ggtataggtg gaataagtat
     9361 ggaaaacaat ctaagaaaaa acaattcaat aatgaaaaag atgatatttc ccctaattat
     9421 tgttatgcta attcaaacaa gtttattttg tgccacaatt ttatggggag gaactattaa
     9481 gaaattaaat gataattcgt ttgacatctt aaatgaaaga gtaattaatc gtaaaaatta
     9541 tatacaaaat gaaatgttac aaagatggtc aaatacaagt gaaacggaag ctacaattaa
     9601 ttcctctgta aaaaaaatat taaatgaaaa aaatgcctta attgaggatg taggcaacaa
     9661 tgaagaaatt aacaaagata ttataaaaag catatcaaat gatttaatat atcttttgag
     9721 aaaaaactct gttacaggag cttttataat tctaaatgac aaaaatatta acgataagcc
     9781 aatagatagt aaaggtgaaa taagtaggac tggattgtat ataagggatt tagaccctaa
     9841 gtttaatcca aatgataatt ctgatttgtt aattgagaga ggttcatcag atatagcaag
     9901 aagttttgga atatctatgg acagttattg ggaacctaaa ttttcattta aaagtgaaaa
     9961 tgagcaagag ggagatgatt ttttttataa accatttaga gctgcaattg ataatccaaa
    10021 cgtatcaagt tctaatttgg gatactgggg aagtaagttt ttattgacaa atgcaggtga
    10081 taggcttatg acatatacag ttccccttat ttatgaagat ggaactgtat atggagtact
    10141 tggaatagat ttaagtgtaa attatttaag agaattactt ccatactcag agataaatgg
    10201 aaataaaaaa ggtaattata ttttaggtgt agatagaaat aatgatatgg cctttgaaaa
    10261 tgtaatttca aatggatact cattaaaaga aaaattttgg gatggtttac attctaattt
    10321 agagcagaag gaatcgtata aaaatatata taagattaaa aataaagata atgaatgtgg
    10381 taatggcaac atttatgcta gtgtacaata cttagattta tataactcca atactccatt
    10441 tgaaaatgaa cgttgggctc ttttgggtat tattgaagaa aaaagtcttt tgtcattttc
    10501 aaaaaggata gagaaattag ttataatatc tatagcaatt tctttattaa taggtctaat
    10561 aggtatttac attgcatcaa gattatttac aaaaccaatt gttcttttag ccaacaaagt
    10621 tagaaacagt aatccatcaa aaccagtaaa tcttgataag ataaatatat ctgaaataga
    10681 tgaattatct tcagctatag agttactcag tgcaaatgtt gcagattctt catcaaaatt
    10741 atcacagatt attgaaatgg tagatatgcc aatcggggct tttgagttat acaaaaacgc
    10801 agagaatgtt ttttgtacaa aagataaaaa acttaagtgg ataagaatga atatctttga
    10861 agatgagcta aaggttcttg gtgttgttgt tgatgtcaca aaagaattta ttgagaaaag
    10921 gaagatagaa tatgaaagag attttgatat tttaacgaat cttttaaata ggagagcttt
    10981 tcattcacag atacaagaaa aatttaagaa taaagatgaa cttaagatag cagcatttat
    11041 tatgtgggat ttagataatc taaaatatat taatgataca tatggacatg atgctggtga
    11101 tgaatatatt aggtgtgctg cagacatact taagaagttt atatcatgga atggtatagt
    11161 atctagactt tcaggtgatg agttttatgt atttatatat ggatatgaaa gtaaagatag
    11221 tataagagat attattaatt ctgttaagaa acagatgaat aataccttat taaaacttca
    11281 tgatggaaca gaatttaaaa taagggcatc agcaggaatt tcctggtacc ctgatgattc
    11341 aaccaactat gatgaattaa tgagatacgc agattttgct atgtatcaaa ttaaaaatac
    11401 aatcaagggg gaagttagtg aatttgatat taatatatat aataaggatt cattcttatt
    11461 gagtaataga gaagaattaa acaagttaat tgatggtcaa ttagttgagt atgcatttca
    11521 gcctattgta gattgtaaag gtaaagtatt tgcatatgag gcacttatga gacctaaaat
    11581 aaaaactttt agttcaccaa tagatgtaat tagattagct cgttcacagt ctaaattata
    11641 tcaaattgag cgtattacat ggtttaaagc acttaaatca tttaaaaaat acaaagaaaa
    11701 atttggcgat tgcaaactgt tcttaaattc tattcctaat aataatttat caaaagtaga
    11761 ttttgaggaa attgaagatt tatatggtga atatttaaat agagtagtat tagaaataac
    11821 agagaatgat aagattaatg aagattttat tgagaaaaag aaagcatata tagaccattg
    11881 gaatatagaa attgctttag atgactttgg gacaggttac aacggagatg ctgtactatt
    11941 atatataatg ccagactatg taaagattga tatgtctatt gtaagaggta ttgataaaga
    12001 tgaaaacaga cagaaaatat taaaaaatct tatctcatat tctaacgaaa gaaatattaa
    12061 agtgatatca gaaggtgtag aaacaaaagg tgaaatggaa acactgatta atcttggagt
    12121 agattatatg cagggttact atattagtaa accatcattt atacctgaag atataagtga
    12181 caaaattaaa aaagagctca agatatgtca tgaaaatagg aatgatgtgg tttaatagag
    12241 gaaaaattaa atttattttt atgaactttt agtttataat taaaaagaaa ataatataat
    12301 tataaattaa ggtggtggag ctaataatca caacaaacat agatataaga aagttaaatg
    12361 aaaatcaatt agaagcagtt gaacatatag atgggccttg catgatactt gcagggcctg
    12421 gttcaggtaa gaccagagta attacatata gaatagccaa tatggttgta aacaaaaata
    12481 tagcaccaac aagaatcttg gcaataagtt ttaccaaggc atcttcaata gaaatgaaaa
    12541 atagagcttt gaatttaagt gatgatataa gacttaataa ggtaacctat ggtacttttc
    12601 actctgtatt ttttaaaatt ctaagatact ttgaaagata caatttagac agtatatttg
    12661 atgaaaagtc aaagcgaatg acaatcaagg caatcctaaa aagtttaaat atagagaatg
    12721 ctgatgatga tgagaatata ggacaagtta taaatgaaat ctcctatgta aaaaatgaac
    12781 ttatggataa aaatgaattt aattcagaag tattaacaaa agatgagttt ttaaaagtat
    12841 acaacttata cgaagaacaa aaatctaaag ttaacaaaat agattttgat gatatgctta
    12901 taagaactta ttatttactt ttgaacaata agtctgcact agaaatggtt agaaatgtgt
    12961 ataaatatat attgatagat gaatttcaag atataaataa agtacagttt gaagttttaa
    13021 agttaatttg tagcccatta aacaatatat ttgcagttgg agatgaagac caaagtatat
    13081 atggatttag aggtgctaga ccagattttt tgcttgaatt tgaacaatac tttaataata
    13141 ctaaaaaaat aatattagat ataaattata ggtctaaaag tgagatagtg tatactgcaa
    13201 atagacttat cgacaaaaat aaaaatagat atgaaaaagt cattaagtgt agtcaggggg
    13261 atggaggaag tgttacctac atttctccac atgattcaga ggaagaagcc ttatatattg
    13321 ccaaggaaat aattgacgaa atacaaaaag attatgtaga gtattcagat tttgcagtaa
    13381 tttatagaac aaatatacag tcaagagcat tagtagatgt atttatggat atgaggattc
    13441 catttgtagt aaaagactca gtaattacaa tatatgacca ttgggcaagt caagatatac
    13501 tagcttattt aagaataggt attaatccaa aatcaaataa agattggttg agaattataa
    13561 acaagccttt tagatatata tccaaagaca gtataaatat ggttaaggat gaaaaagact
    13621 ttataacagc attaataaat aagtgtaatc ttcatcctaa acaagttaaa acaataaatg
    13681 atttggaaat agatttaagt tatttaaata ctttaaatcc taaaaatgca atatcttata
    13741 ttagaactag tcttgattat gatagatacg ttttagatta ttgtagcaac agaaagataa
    13801 aaactaatgg acttgtagag atattaaatg aacttgagag ttcagcaact aattttaata
    13861 ctgttacaga gtttttagag catattgata gagtaaagac tgagttaacg gaaaataaca
    13921 agaataagca gactgagggt gtcatattta caactatgca tagtgcaaaa ggattagaat
    13981 ttagaaatgt ttatattata ggtgtaaatg aaggaacaat acctcatgaa aaatcatatg
    14041 atatttgtaa ggaagagaaa aaagaagaac aattagaaga agaaagaaga cttatgtatg
    14101 ttgctattac aagagctgaa gaaaagttat gtataagctc tactttaaat aagtatggta
    14161 agaaagttga taaatcatta tttattaatg atataaaatc tccaacgaaa aaagaaattg
    14221 acagtattgg tgtaggggat aaggtatacc ataaaaaatt tcatgaagga gaaataatta
    14281 aaaaagatgg aattatgttt acaataaagt ttaaagatag agagcgaatc ttggatttga
    14341 aaacttgttt attgaagaat ataatttata ctatttaatt tagaatctaa atttagatag
    14401 aaatataatg tattatttat tatgtgcaag acaaatagat atgtgtaaaa tgtattattt
    14461 aacttattag aaaaagaaat aaggatttaa tatattattt atagttgttt agaaaaagca
    14521 acaatgtgga aaatattatt tagtattatt aaagaaaagc aacaatgtga aagatattat
    14581 ttagtgtaat ttaagaaaaa gcaacaatgt gaaagatatt atttagtatt attaaagaaa
    14641 gagcaacagt gtgaaagata ctatttagta ttattaaaga aaaagcaaca gtgtgaaaga
    14701 tattatttag tgtaatttaa gaaaagcaac aatgtgaaag atattattta gtattgttta
    14761 agaaaaagca acaatgtgaa agatattatt tagtattatt taagaaatag ctaaaaatat
    14821 gaaatatact atttataatt acaaaaataa tatcaataaa tataaacagt agtatttaga
    14881 taatttacaa gtaaaaatta ttaaaaaaat aaaaaactta ttgacaaaaa tattataagg
    14941 aagtacaatt gtattaacaa tttaaggaaa caaatccaat gatagagaaa gtagcatata
    15001 tggatttaca gcgagttagg ggttggtgta agcctagcaa tgaagtctat gtgaagagag
    15061 ctctggagga gattatcaga aactttagta tgataattgg tttacccgcc ttaagggatt
    15121 gagaggataa gtacttactt atcaaaaaga gtggtaacgc ggatataatt cgtctcttag
    15181 ctgtaaagct aagggacttt tttgatttaa aaataaaaat atccatacag cttaaatgct
    15241 aacagtcaca aattttgaaa gtaatataag tatttatcaa taagagtggt aacgcggtaa
    15301 agtcgtctct tagtcattaa agactgagag actttttttg taactcgaaa catttaagta
    15361 ttaaaatctg agggaaatta taattaaaag agaggggtag ttaaaatgaa agtatttaaa
    15421 aaattattaa gtttaggatt agtattagga ttgacattat cgctagtagg atgtagtggt
    15481 ggaggagaaa aaacaaagtt agaacaaata aaagacaatg gaaaattagt tgttggtact
    15541 agtgcagaat ttcctccatt tgaatttcat aaggtagtag atggaaaaga ttcaataaaa
    15601 ggattcgata taatgttggc tgaagaattt gcaaaagagc ttggtgtaaa ggttgaaatt
    15661 aaggatatgt catttgatgg attaataggt gctttaaatg cagaccaagt tgatattgtt
    15721 cttgcaggta tgtctccaac accagagaga gaaaagagtg ttgatttttc agaattatat
    15781 tatttaagta gaaatgcagt tatagttaaa gatgcagata tagacaaagt aaaaactgaa
    15841 gatgatttaa agaaacttag agttggagtt caagcaggaa gtatccaaga agaatatgta
    15901 gttaacactt taaaaatgac aactacaaaa tctttaaaag caatacctga tttaattaca
    15961 gaattaaaaa atggaaacat agatgcagtt gttactaatg aagcagtttc tttaataaat
    16021 gttaaaaaat atgatggaat aaaaatggca aatactgaag ttggtaaaga tgtaacagaa
    16081 ggtatggcag cagcaatcaa aaaatctgac aataacaaag attttataga attattaaac
    16141 aaaaagataa aagaattaca agatggtaag aagatagaag aatttttaaa tgaagcatct
    16201 actgaagctg cttcaaatta gtaagaaagt tatgtgggag atgaaattat gagtttagat
    16261 tttagttttt taagtagatt tgggacttct tttttggaag gaacaggtgt gacagtatca
    16321 atttctcttg tggcattatg ctttggattt ataataggta taattatctg tatggcaaaa
    16381 atatcaaaga gcaaagtatt aagagcaata tcatcaatct atattgaggt tttaagaggg
    16441 acaccattat tagtacagat atatatagta tggtttggtt tacctcaatt aggaataaga
    16501 ttccctatgt tgtttggtat accaagtgaa tttatagcta gtgcatttgc attatcagta
    16561 aactctggtg catacgtggc agagatactt agatcaggta tacaatcagt tgataatgga
    16621 caaatggaag caagtagatc attaggatta aattattggt caactatgag atatataata
    16681 atacctcaag ctataaaaaa tatattacct tcattagcaa atgagtttat tacacttgta
    16741 aaagaatctt caataatatc tgtaatagga gttgttgaga taatgcgtac tgctgatata
    16801 gtaaaaaatg cagcatttag agcattagag ccattaatag tagcagcagc aatttacttt
    16861 gttataacat ttactctatc aagattagtt ggattattag aaaagaaact atctgtgtct
    16921 aactagatga gagcatgttt agcatttagg gaaaacatag aatagtaaaa aggaaagtga
    16981 taatatgatt acaataaaaa atttaagtaa atcttttgga gatttaaatg tattaaaaaa
    17041 tatagactta gaaatcgcta aaggcgaaat aatggttata gtaggtccaa gtggttctgg
    17101 taaaagtaca cttcttagat gtatgaattt actagaaata cctacaggtg gagaaataat
    17161 atttgaagga aaaaatttgg tcgataaaaa aactaatatt gatgaggtta gacaaaatat
    17221 aggtatggtg tttcaaaact ttaatttatt cccacataag actattttag ataatataac
    17281 attagcacca attaaattaa aaaagatgac taaggaagaa gcagaaaaaa aagcagaaat
    17341 tttgttgagt agagttggtc ttttagataa aaaagattca tatccatcac aattatctgg
    17401 aggacaaaag caaagaatag caatagctag agcacttgct atggaaccag atatgatgtt
    17461 gtttgatgag ccaacatctg ctcttgaccc agaaatggta aacgaagttt tggatgttat
    17521 aaaagaactt gctaaagaag gtatgacaat ggcaattgta actcatgaga tgggatttgc
    17581 taaggaagtt gcagatagag ttatttttat agatggagga agtatacttg aagataatac
    17641 tccagaagaa gttttcggaa atccaaaaca tgaaagaaca aaagcatttt tagcaaaagt
    17701 gctataatat ataaaaagga tgttattgat attagaaatt caatacatcc ttttttagtg
    17761 atggttaata ttaattttaa aattaaagtt atattaatat taactttatt tttacgattt
    17821 tttgcagaaa aaaaaagagc tcggggtaag ctcttttttt ccaccgaatt aatttccgtt
    17881 actaatccga tttttaataa atataaaata tatccttatg aaagggtatt gggaatagat
    17941 atattaacta actagggtta ccagggggat aaccttaatt tattataaca aaatgcgaca
    18001 caaaaatcaa tatttttttt gaaataaatt ataaaagtta tttttataac aagtaagctt
    18061 ataaatttaa cttttatagt aaaaataagt atatgtataa tataatatat ttattttata
    18121 taatatatta ttttatcata tattgtattt tataatatat ttttgtaagg tatagtataa
    18181 gtatattata ttttactata aaaatttgta ataaagtgaa aaaactagct tttcatgaaa
    18241 taatatttta taatttaaga ttgtcttttt aataaagatt aacaaaaaaa atactataat
    18301 tagtggtata atagttagag ttgaagattt taaataaagg taggtgtaat taagttggaa
    18361 atatacttag acaatagcgc aactacaaaa ccttatcaag aagttataga taaaatggtt
    18421 tatgcactta atacggaata tggaaatcct tcttctgtcc atagaaaagg tgtagaagtt
    18481 gaaaaagcta taaaagaagt aagacaagat atagctaaat ctctaggagc gaaagaaaag
    18541 gagatttatt ttacatcagg tggtacagag tgtaataata ctataataag aggtattact
    18601 agtttaaata aaaaaagaaa aaatcacata atatctacta acatagaaca tccttctgtt
    18661 ttaaatacat taaaagattt agaagaagat ggttttgaag taacttattt agaggtcgga
    18721 aaagatggga aaataaatat agaggattta aaaaatgcga taaagtcaac tacatgctta
    18781 gtaagcatga tgcatgttaa caatgaaata ggaactatac agccaatagg tgaagttgga
    18841 aagtatttga agggtttaaa agaaaaaatt tatttccatg ttgatgctat tcagtcatat
    18901 ggaaaaatta attttagacc atctaaatac aatatagatt ttatgagtgt aagtgcacat
    18961 aaatttcatg gtccaaaggg aattggattt atgtatataa aagaaaataa tcgccttaag
    19021 cctatgttaa caggtggtgg tcaggaaatt ggaattaggt cgggaacaga aaatgtacct
    19081 ggaatatatg gtcttggaga agctgttaga atactaaaca aagatttaga tgttgttatt
    19141 tcgaaagtaa ataacttaaa aaatatatta aaaaatgaaa taattgataa tatagaagat
    19201 ataaaaatca attctccaga agatggagta tgtcatatat taaatgtatc atttagaggg
    19261 acaaaaggag aagttttact tcattaccta gagcaaaaag gtatttatgt atcaactggc
    19321 tctgcatgtt cttctaaaaa gaaaggtagt tacgtattaa atgctatagg tcttacaaat
    19381 gaagaaataa atggaacaat aagatttagt ttatctgata tgaatacaga agaagaaatg
    19441 ttagaagctg tgaaagtttt gaaagaatca atttgtgact taaggtcaat aatgaaaaga
    19501 aaataaagaa tactgtatag atagtgataa taaattagtt taagaggtga aatggtgtat
    19561 aatatattaa tagtaaagta tggagaaatt ggagtaaaag gaaagaatag atatatattt
    19621 gagaataggc ttataagaaa tataagaaat atgttaaaac caattggaag atttaatgta
    19681 tataaagagt atggaagaat atatgttgat ttagaagact atgattatga agaagtaatt
    19741 gaagaagtta gaaaagtatt tgggatagtg ggtgtgtgcc ctggagtaag agctaaaaaa
    19801 gattatgata cattaaaaga aatagcttta aaaatgttag aagaaaaaat agaggctgga
    19861 tataaaactt ttaaggtaga gtcaagaaga ggagataagt ctttcgggtt gacttctcaa
    19921 gaaatgagta tggatatagg tggatactta ttatcaaagg taggagatag gattaatgtt
    19981 gatgtaagaa atccagaagt taaaataaag tgcgaataca gagaatttca tactatggtc
    20041 tatagtgata ctatacctgg atatggtgga ttgccacttg gaacgaatgg tagagctatg
    20101 tctcttttat caggaggtat agatagtcca gtagctacat ggatggttgc aaaaagaggt
    20161 atggaggtag aggcagttca cttccatagt tatccattta caagtgaaag gtcacaggaa
    20221 aaagttaagg atttagctaa aatactggca aaatactgtg gtagagtaag acttcataaa
    20281 gttaacatat tagaaataca aaaagcaatt ggtgagaatt gtaatgaaga agaagctact
    20341 attctttcaa gaagatttat gatgaggata gcacagagac tttctgaaaa aagacattgt
    20401 gatgctttaa ttacaggtga aagtattggg caggttgcat ctcaaactat acaagggctt
    20461 acttgtacta atgccgttgt agacttacca gtgtttagac cacttatagc aatggataaa
    20521 tcagatatag ttgatatagc caaaaaaata gggacttttg aaacatctat agtgcctgaa
    20581 gaagattgtt gtagtgtatt ttctcctaga aagcctgtca ctaaacctag attagagaaa
    20641 atagaaaaat ctgaaacagc acttgatatt gaaaaattag tacaggatgc tattgacaaa
    20701 atagaggtag aggatattga gttttagcat acaagttatt taattactaa attgaatatt
    20761 gagtatatta gtagttctgt aagtatatta gtagttctgt tgaaaggatt tatgtagaca
    20821 atttattaat atatagtatt taaaaataag tactataatt ttatttaatg ggaatgatta
    20881 ttatgaataa aaagggtttt acattaattg aattgttggt agttatatct ataataggaa
    20941 ttttagttat agtagctgtt ccagcgttat ttagaaatat agaaaaaagt aaggcagtta
    21001 catgtctttc taatagagaa aatataaaga ctcaaattgt tattgcaatg gctgaggaat
    21061 caagtaaaga caagaatgaa gtcataaaag aggtattaga aaacaaagat ggtaagtact
    21121 ttgaaacaga accaaagtgt aagtcaggtg gaatatattc agcaacgttt gatgatggtt
    21181 atgatggaat aactggaata gaaagcattg caaaagtgta tgttacttgt acaaaacatc
    21241 cagatggtat tgaaatggct agggatatac atcaaagtat gaaagatttg attgcatcat
    21301 ttgcacaaga cccttctata ataccaggag cttcaaaggg caatgatgat tttagaaaat
    21361 atttattaga caataaatat aaaaatgggt ggcctacaat tccagatgaa tttaaggcaa
    21421 aatatggatt aagtaaggat acactatata tacaaccata tgcatataat cctactaaat
    21481 ctgatgctac tgtagttgta tttgcaaata ataagactgg aggtaattgg tatacttccc
    21541 tagtttacga ttatgatgaa ggtagatggt ataaaggtaa aaatggtatt tctgttgcag
    21601 gtaggtcatg ggatgttgac acagatagtg ttaagtctgt aaaaacagag attcattcta
    21661 aagagggatg gggtccttta aattaatata tcatgtttta ttaatctggt attgattaaa
    21721 ctatagaaaa gagcagtttc tatagatgac gaataccaga ttttcattta tatgtataat
    21781 taagatatat taaaagtatt tatattttta agtcttcaca tacaagctaa agataattat
    21841 aataatattg taaagaaatt taattataag attaattttc tgaagattta gtatttgtac
    21901 taagaaatac ataaaaaggg gaatagatat gaataataat tttcaagaca accaaacaat
    21961 aaatttaata caaagtcgta gaagtatacg aaaatttaca acagagcaaa ttagtgatga
    22021 gcaagtaaac acattattac actgtgcatt tgcagcacct tcaggatgta ataaacagcc
    22081 ttggcacata actgttgtgc aagaccagaa acttttaaaa gagattagtg atgatacttt
    22141 atctaggatt catgaagtga gtaatgttga gataaataaa aactttaagt tattttatgg
    22201 tgctccaacc gttttattta tttcttatga tgaaagtagt tcatgggctc catatgatat
    22261 aggaatactt acagggaata taacaacagc tgcacaagca ctagggcttg gaagttgtat
    22321 aataggaatg gttagaggtt tatttacacc tgttgaacaa ggggatattg aaggtcttgt
    22381 gagtgtattg gataaagaag atgtaaagga atcagagtca ataaagatga agtttgatac
    22441 aaataaaaaa tacagagaac tacttgatat accagaaggg tattctgtac catttggtat
    22501 tgctgtaggt attccagatg gaaatttacc aaatgctaga gaggtagttt acaaagtttc
    22561 cagagtttag gataaagtat tgcaagaacc aatcagttac ttgaagaatt aaaccagtaa
    22621 tatacaaata aattgatgga agaggaagct atttaaaaag tagtttagat ttttcattgt
    22681 aaaataatta aataaagaat aaggtgataa gtattttatt tattatctta ttctttttta
    22741 tttttgtttt gtagaatgat aaaaataagg attaagtgta ataaaataga aaacaagtat
    22801 gttaatttta agtatatgct ttaaaattta tccaagttaa aaatttaaga tatcttttca
    22861 gtataatgga aaaaatgtca aaatattgaa atagggaaaa aatagtgata taataaaaaa
    22921 aatagagaaa aaaagtaacc cttagttgta aaagggttct ttt
//
LOCUS       DAEPOZ010000033        22811 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.033, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000033 DAEPOZ010000000
VERSION     DAEPOZ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22811
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.033"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            89..553
                     /locus_tag="KR440_001060"
     CDS             89..553
                     /locus_tag="KR440_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBH0698118.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            560..1054
                     /locus_tag="KR440_001061"
     CDS             560..1054
                     /locus_tag="KR440_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HBH0698119.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            1070..2095
                     /locus_tag="KR440_001062"
     CDS             1070..2095
                     /locus_tag="KR440_001062"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HBH0698120.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            2082..4529
                     /locus_tag="KR440_001063"
     CDS             2082..4529
                     /locus_tag="KR440_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBH0698121.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            4709..6082
                     /gene="radA"
                     /locus_tag="KR440_001064"
     CDS             4709..6082
                     /gene="radA"
                     /locus_tag="KR440_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBH0698122.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDENIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            6085..7155
                     /gene="disA"
                     /locus_tag="KR440_001065"
     CDS             6085..7155
                     /gene="disA"
                     /locus_tag="KR440_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HBH0698123.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            7244..8338
                     /locus_tag="KR440_001066"
     CDS             7244..8338
                     /locus_tag="KR440_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBH0698124.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            8467..10644
                     /locus_tag="KR440_001067"
     CDS             8467..10644
                     /locus_tag="KR440_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698125.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDMSTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSENGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVVFVELGDTYRLDLYKPNLNEKTYESVKDNIEKNIDVYLKNIFSMVEEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVLFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
     gene            complement(10830..11675)
                     /locus_tag="KR440_001068"
     CDS             complement(10830..11675)
                     /locus_tag="KR440_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0698126.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCTYQDTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSGKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKKST"
     gene            11892..13058
                     /locus_tag="KR440_001069"
     CDS             11892..13058
                     /locus_tag="KR440_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HBH0698127.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFATIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNIVLDTHQYLMLAESDGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLGKCVAQEWYPIEY"
     gene            13080..14726
                     /locus_tag="KR440_001070"
     CDS             13080..14726
                     /locus_tag="KR440_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBH0698128.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYIHSSGFDASILHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWRPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNVLGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYIQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIADESKDVD"
     gene            14823..16196
                     /locus_tag="KR440_001071"
     CDS             14823..16196
                     /locus_tag="KR440_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0698129.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNYIG
                     VIFASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMFFYKLDKEYSTI
                     LNELNTK"
     gene            16641..17594
                     /locus_tag="KR440_001072"
     CDS             16641..17594
                     /locus_tag="KR440_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBH0698130.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            17632..18594
                     /locus_tag="KR440_001073"
     CDS             17632..18594
                     /locus_tag="KR440_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HBH0698131.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            18619..19605
                     /locus_tag="KR440_001074"
     CDS             18619..19605
                     /locus_tag="KR440_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBH0698132.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            19618..20664
                     /locus_tag="KR440_001075"
     CDS             19618..20664
                     /locus_tag="KR440_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HBH0698133.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYSGKIRKCDVEKLTAKETIVSNNTSKYSE
                     KKELKIENENSSMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            20831..22561
                     /gene="lpdA"
                     /locus_tag="KR440_001076"
     CDS             20831..22561
                     /gene="lpdA"
                     /locus_tag="KR440_001076"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBH0698134.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDIV
                     EENQNIEVKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEVPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
BASE COUNT         9229 a         2380 c         4326 g         6876 t
ORIGIN      
        1 atacaattta gggctttaaa aatttaaata ataggtgtaa aataatagtc aaagaaagtc
       61 aaaaataaaa aggtgtgtgg tgatattaat ggcaactatg acagatataa ttgaaaaatt
      121 tattaaagat ttgatggaag aagataatag catacaaatt caaagaaatg aactagcaaa
      181 cttgtttagt tgtgcaccat cacaaataaa ttatgtctta acaacaagat ttacaataga
      241 tagaggatat tacattgaaa gtaaaaaagg tggcggagga tatgttcaaa tagaaaaaat
      301 acgaaaaagt aaagatggac atattagaga actactaaat gaaaaaatag gaagtcagat
      361 atcctataaa aaagcaaagg aattattaga aggtttaaaa gagtcggatt tgattaacga
      421 aagagaatta aaattaatat tatatgcaat agatgataaa tcattgtgta tgccaatata
      481 tgaattaaaa gagaaggtaa ggtctaatat tttaaagaat ataataatag gattatttag
      541 tatagaggag tgataattta tgctatgtca aaaatgcaat aagaataaag catctgtata
      601 ttataataag atagtcaatg gagaaaaaac agagatgtat ttatgtagtg agtgtgcaaa
      661 agagaataca gaaatgaatt ttaacttaga tatgcctttt tcaatgatgg atatattttc
      721 taatttaggt tttcaaccta aaaaagaact tgaggaaaag ctagtttgtc ctaaatgcaa
      781 tacaacatat agtgagttta agaataatgg gagatttgga tgtagtgaat gttataatgc
      841 atttagtagt caagtaaatc caatgcttca aaatatacat gggcatatag aacatacagg
      901 aaaagcacct aaaaaatctt tttataagat aagtgtagaa aatgagatta aagaactaaa
      961 agaagattta gatagagcta taaaaaatga agagtatgaa ttagcagctc aatttagaga
     1021 taaaattaaa tatttaaaag gttctattga ctaagattgg agtgatatta tgaaagagaa
     1081 catagtcatg aaatcaagag ttaggctagc tagaaattta aataactatc catttccaaa
     1141 taaattagat aaagaatgtg ccatggaaat aatagaaaaa gtaaaaaatg cgtttattaa
     1201 ttctaactta gaacaaaaag aagaatttga tttctataaa attgaagatt tagaccaatc
     1261 taaaaaaatg cttatggttg aagaacatat aataagccca gatttagcag aaaatgataa
     1321 aagtgcagtt atagtaaaaa aagataaaac tataagcata atgataaatg aagaagacca
     1381 tataaggata caaactatat gtgatgattt aaacttagaa tatgcatata gtgtagcaaa
     1441 tgaaatagat gatttgttag aatcaagttt agaatatgcc tttaacacaa agctaggtta
     1501 tctaacatct tgtccaacta atacaggaac aggaatgaga gcttctgtaa tgatgcattt
     1561 accagcatta agtcaactag gctatatgga tgaactttat aaaatttcat cacaaatagg
     1621 tatagcaatt agagggatat atggagaacg aacagaagca ttgggaaata tatatcaaat
     1681 atctaatcaa cttacacttg gaagaacaga gtctaatatt atagaaaatg taagtggact
     1741 tacaaaagat gcaatttcaa aagaaataaa agctagagaa atattgcaaa aaaaattagg
     1801 tataaaacta gaagataaaa tatttagatc gatagggaca cttgaaaatt caagagtaat
     1861 gagtagcgca gaggcaatga gccatctatc aaatataaaa atgggaatag aaatgaacta
     1921 tatagataaa ctagatttaa gggctataga acaacttatg ataggtatac aaccagcgca
     1981 tcagtcaata atgtataaga gtgatgatgt tgagaataga gatataaata gagcaacata
     2041 tattagagaa actttggaaa aattaagagg agggatgaat tgtgaacttc aatagattta
     2101 cacaaagagc taaaaaggca attgatttag cgtttgaatc tgctaaaagt ctaggacaca
     2161 atattgttgg aagtgaacat atacttttag gtcttttaag agaagaagaa ggtatagctg
     2221 ctaaagttct aagtaaagtt ggatttacag aagcttattt agaaggcaaa atagttgata
     2281 tggaaggtaa gggagaagaa atctcagaag atatagtatt aagtccaagg agtaaacaaa
     2341 tacttgaatt atcaggaatg tttgcaaata aattaaaaac aaattatatt gggactgagc
     2401 atatattatt agcaattatt caagaaggtg aaggaatagc taataagatt ttaaattatg
     2461 ctggagtaaa tgataggact ttagcccaat taacaattga tatgatgggt attagtgata
     2521 aaaatcaata caaagcagag aatagttaca cgggcaacca gaatcaagca gaatctaaag
     2581 tacttgataa gtatggaaga aatcttacac tatatgcaaa acagaataaa atagaccctg
     2641 taataggtag agaaaaagaa atacaaagag taatacaaat attaagtaga agaactaaaa
     2701 acaatccagt gttgatagga gacccaggtg tagggaaaac agccatagct gaaggtctgg
     2761 ctacaaatat agccttagga aatgttccag aaacactaaa aaacaaaact ttatactctt
     2821 tagagatggg ttcattattg gctggggcta agtatagagg agaatttgaa gaaagaatta
     2881 aagaagttgt agatgaagtt gttaaaaatg gaaacataat cttatttata gatgagatgc
     2941 acacaataat aggggctggg tctactggag aaggctctat agatgcttca aatatattaa
     3001 agccagcctt ggcaagagga gaaatacaag ttataggtgc aactacaatt gatgaatata
     3061 gaaagcatgt tgaaaaagat tctgctctag aaagaagatt ccaaccagtt atggtagatg
     3121 aaccaactaa agaggactca ataaaaatat tagaaggatt aagagataaa tatgaagctc
     3181 atcataaagt taaaataact gatgatgcta taaaggcagc tgtagaatta tcaactagat
     3241 atatatcaga cagatatctg ccagataaag ctatagactt gattgatgaa gctgcatcaa
     3301 aagtaagatt aaaagaaaat acacctcctt cagaaataaa aaaattagaa ctagaaatag
     3361 aaaatataga taaagaaaaa gaagaagcag taagatgtca agactttgag aaagcagcca
     3421 aaataagaga tgaacaaggt ctacttaaaa aacaattaga agatgttaga gaaagatgga
     3481 acaagtcatc taaacattca gatttagttg atggagaagt tattgctgaa gtagtgggct
     3541 tatggacagg aataccagtt aataaaattc ttgaagaaga ggctgataga cttttgagac
     3601 ttgaggaaat actgcacaat agagttatag gtcaagaaca agcagtaaaa tctatttcaa
     3661 aggcaatcag aaggtcaaga gcaggtctta aagaccctaa tagaccaatt ggttcatttt
     3721 tatttttagg tccaacagga gtaggtaaaa cagaattatc taaggcatta gcagaagtgc
     3781 aatttggaga tgaaaatcaa ataattagaa tagatatgtc tgaatatatg gaaaaacatg
     3841 ctgtgtcaag aatgataggt tcacctccag gttatgtagg tcatgatgaa ggtggtcaat
     3901 taactgaaaa agtaagaaga aatccatatt cagttatctt atttgatgag atagaaaaag
     3961 ctcatccaga tgtatttaat attctgttac aaattctaga tgatggtaga ctaactgatt
     4021 caaaaggaag aactgtagat tttaagaata ctattgtaat aatgacatca aatgttggag
     4081 catctacaat tggtagacaa aaaactttag gatttagtat agctaaagga gacgaagaag
     4141 aaaaatctca atatgaaaaa atgaaagaaa atataatggg tgaattaaaa caaagattca
     4201 gaccagagtt tttaaacaga atagatgata taattgtttt ccactcacta aatgaaaatc
     4261 atatatcaaa aatagtactt ttaatggcag cgaaactaca agagagatta aaagaaatgg
     4321 atataaaatt agaaatgagt gatgaggctg ttaaattaat ttctaaatct ggatttgact
     4381 tagaatatgg agcaagacct cttaaaagag ctttacaaaa agagttggaa gatgagctat
     4441 ctgaagcaat cttaaaaggt aatgtgaaaa agggaagtaa tgtagtagca aaagtcaaag
     4501 atgaaaaaat agtttttgaa actaaataat ttaatagagt tgcattttaa aatattgcaa
     4561 atataagttt tataatctaa acaaaatttt aagggcaatg aatctataat aattcattgt
     4621 ccttaattat tttatttaat ctttacatat tgtctttcag atatgctaaa attatagtat
     4681 agaataaaaa attaaggatg ataaaagtat ggcaaaaata aaaacaaaat atgtatgtca
     4741 atcttgtgga tatgaaacag ctaaatggct tggtaaatgt ccagaatgta caaaatggaa
     4801 tacatttgta gaagaaatag accaaaaaag caccaaaaaa gaggtcttta taatagataa
     4861 atcttcatct aaaccagtca gtataaactc aatagaaagt aaagaagaag agaggtttac
     4921 aacagatata aatgaattag atagagtgct tggaggaggt atagttaaag gctctttagt
     4981 gcttgttggt ggtgaccctg gtataggtaa atcaacactt ttaatccaag tatctagtaa
     5041 tgttgccaat ttaggaaaaa ctgttttata tataactgga gaagaatcag aatctcaaat
     5101 aaaaatgaga gctaaaagat taggtattaa ctcagaaaat ttatatatat ttgctgaaaa
     5161 taatttaagt ataattgaat cttatttaga aagtgtaaat ccagaattaa taattctaga
     5221 ctcaattcaa actgtcttca gtccagaaat atcctcggca ccaggaactg tcagccaaat
     5281 taaagaaggt acttcaaaat tcatgaaaat ttctaaaaaa atgggaattt caacgttcat
     5341 agttgggcat gttacaaaag aaggttcact agcaggacca aaattattag agcatatggt
     5401 tgatactgtt ctatattttg aaggagaaag atataatact tatagattag taagagctgt
     5461 taaaaatcga tttggttcaa ctaatgaact tggagtattt gaaatgaggg atttaggatt
     5521 agtagaactt gataatccat ctaaaatttt aatctctgaa aaaccaaaag atgtagcagg
     5581 ttcagtaata atatctactg tagaaggtac gagaccaatg ttacttgaat tgcaagcatt
     5641 agtttctcct acaagttttg gtataccaaa aagaacatct acaggagtcg attacaatag
     5701 agtaggtatg ttattagcag tattagaaaa acgtgttggt ttgcaaatac aaaatcaaga
     5761 tgtctatata aacatagttg gaggaataaa gataaatgaa ccctcaattg atttagggat
     5821 agccatttct gtagcatcta gttttagaaa tataccgata gatgaaaata tagctgttac
     5881 aggagaagtg ggacttacag gagaagttag agcagtaagc tttatagaaa agagaatagc
     5941 tgagtgtaaa aaacttggat ttaaaaagat agtagttcct agaagtaatt atgatgttgt
     6001 aaaagagacc aaaggcatag aaatatggcc agtagataac ttaagacaag ctataaatat
     6061 agtacttggg aggaatcaat aaatatggag aattttctag ataataaaaa tatgctatat
     6121 gcattaaaaa tgatatctcc tggaactcca cttagattag gtctaaacaa tgtactaaga
     6181 gctaagactg gtggattaat tgtaattgca acaaacgaag atgtaatgaa aatagtagat
     6241 ggaggatttg ctataaatgc agaatattca ccatcatatc tatatgaatt agctaaaatg
     6301 gatggagcta tagttttaag tggtgatgta aagaaaatat tatttgctaa tgcacaactt
     6361 atacctgact attttataga aacatcagag acaggaacaa gacatagaac agcagaaaga
     6421 gtagcaaaac aaactggtgc tatagtcata ggaatttcac aaagaagaaa tgttataaca
     6481 gtttatagag gaaatgagaa gtatgtagtc gaagatatat ctaagatatt tactaaggca
     6541 aatcaggcta tacaaactct ggaaaaatat aagacagtat tggaccaagc tgtaacaaat
     6601 ttaaatgcct tagagtttaa tgatttggta actatttatg atgttgcatt agtcatgcaa
     6661 aagatggaaa tggtaatgag agttacaagt ataattgaaa aatatgtgat agaattgggt
     6721 gatgaaggaa ctttagtaag tatgcaatta gaagaattaa tgggtacaac cagaatagac
     6781 cagaaattaa tattcaaaga ttataataaa gaaaacacag aaataaaaga acttatgaaa
     6841 aaggtcaaaa atttaaattc agaagaacta atagaattgg tcaatatggc aaaactatta
     6901 gggtatagtg gtttttcaga aagtatggat atgcctataa aaacaagagg ttataggatt
     6961 cttagcaaaa tacatagact accaacagca ataatagaaa acttagtaaa ttattttgaa
     7021 aactttcaac aaattttaga tgcatctatt gaagaattag atgaggttga aggaataggt
     7081 gaaataagag caacatatat aaaaaatgga ctcataaaaa tgaaacaatt agtcttatta
     7141 gatagacaca tatgatgaat tttaaatgaa ttttactact tttttcttga atataatata
     7201 cactttggtc tataatagat aagtataact agggggtgaa gcattgataa ggaaagtaac
     7261 aagaatattg tttattttac ttggatttac aataggtata acaacctatt taacattaat
     7321 gaaagatttt gaaatattaa catttggaaa agaaacatat ggttatatcg cagctgtagt
     7381 agcaggtata ataatagcca ttttaggata tcttatagaa ccatgggttg taaataaagt
     7441 aaaagaaatt gctaaaatag tggataaaga attgtcaaaa tatcctcaga ctgatatatt
     7501 gttagggtct atgggtctta tagtaggatt tgttattgcg tatttattaa gtggattagt
     7561 aaatagaata ccaatagtag gtggaatact atccttacta ttatatttat tcctaggata
     7621 tcttggaatg aaagttgcgc ttaaaagtaa aaatgattta tttaatattg gaaaactagg
     7681 aagattagca aatcctatta aggataaaga taaggataaa gaaaataaaa aagaagtaaa
     7741 agccattcct ccaaaagtat tagatacaag tgtaataatt gatggaagaa tagctgatat
     7801 ttgtaagact gggtttatag aaggaaaact tattattcct gcttttgttt tggaggaact
     7861 aagacatatt gcagattctt cagatgattt aaaaagagta agaggtagaa gaggtctgga
     7921 tatattaaat ataattcaga aagaattaaa tatagaagtt gaaataagtg agagagactt
     7981 tgatgatata gcagaagttg atagtaaatt attaaagctt gctcaggttt taaatggaaa
     8041 agttgttaca aatgattata acttaaataa agttgctcaa ttccaaggtg ttgaagtatt
     8101 aaatataaat gagttagcta atgctataaa accagttgct ataccaggtg aagatatggt
     8161 tgttcaagtg gttaaagaag gaaaagaggc tcaacaaggt gttgcatatc tagatgatgg
     8221 aactatgata gttgttgatg gaggaagaaa atatatgaat gagactataa aagtactcgt
     8281 tacttctgtt cttcaaactc ctgcaggtag aatgatattt gcaaaaccta aaaattaata
     8341 tgttcaaaga aacctataat ttgggctgtt tattttaaat agatggtcca aatttattta
     8401 tttataacga taatgtttaa tgtattctta aagatgggga tttaaatcag aaagcaggga
     8461 gaatttatga gaaaaaagat tatatctttc ttagcaacat ttatcataat attaacatca
     8521 gcaagtcagt atagttttgc agatgatatg tcaacacgtg gaaaagtaat atttatagat
     8581 atgaatagaa ctagtatgag taatatgtta agaataaaat cattaagaga agaactagac
     8641 aatagagggt atattggctt gatgaatata agaggcgata aaggaagtga tgatagaagg
     8701 tcatatgcta gtatgggtgc tggtggtaga gctaatgtgg ctaatgaaga agatattaat
     8761 tttgaaagct cgagtaagga tagaaatata gtttttgaat cagctacagg aaaaagtgca
     8821 aaaggtataa ataatttaac tataaataag tctataaatg aaaatttaaa ctttggagag
     8881 tatggttctg tattagggtc attaggacaa agtttatctg agaatggatt aaaagcatct
     8941 gtattgggta attcagatat aattgaaaat ggtcaactta taaaaaatag aaacttatgt
     9001 ctaactgcaa tggatgaata tggaagaata cctaatggaa atgtagatac tataaataaa
     9061 aaagacttat ctatgcctta tggaatatca actgattatg ataaattgat tgtagagacc
     9121 aaagaagctt acaaaaataa tgatgtggtt tttgtggagc ttggagacac atatagatta
     9181 gatttatata aaccaaattt aaatgaaaaa acatatgaga gtgtgaaaga taacatagaa
     9241 aaaaatatag atgtatattt aaaaaatata ttttctatgg ttgaagaaaa tgacactgtc
     9301 tacatagcaa gtgcatttcc aagtgattta gattataaaa ataaaagaag actttctcca
     9361 ataataaaat taaatggaga agggaaaggt atattatcat cttcaactac tagaagagaa
     9421 ggtattgtaa caaatttaga tgttggggtt gaaatattag acaactttaa tataaaaaat
     9481 caaaatatgg ttggaagaaa atatgaactt attaacagag atgataataa agaatttttg
     9541 atggatgaat atcaaaagat agtatctatt tcaagcataa ggtctacaat actgaatggt
     9601 tttgtaagta tagttttctt gtcatggata gttgctatga ttgcaatttt atttagaaag
     9661 catatatcaa aaaattataa agaaacaaca ttttttatat taaaggaact tttaaaaata
     9721 ggaatagtaa tgccactgtc atttatgata acaccaataa tgaatttcaa aacaccatta
     9781 gcaatatctt taggaataat tataataaca cttactatat atcttataag taaagtttta
     9841 ataaagaatg atttaaaaaa catgctgttt tttactggat taaccatagt aattatggtt
     9901 attgatgctg ggtttggctc atatttaatg aaaagcaatg ttatgagtta tgactgtata
     9961 attggagcta gatattatgg agtgggaaat gagtatcaag gtgtggcaat cggtagtgca
    10021 atatttacat ttgcaatttt acttacatat aaaaatatac caaagtggtc tataatagtt
    10081 ttttctttgg ttatcttaat tacaagtgca tctccaatta tgggagcaaa tgttggaagt
    10141 gctatttctg aatgtgttgc atttttacta tttatactat tgatatataa tgtgaagata
    10201 gacttcaaaa aaattgtatt attaggtata gcagtattat ttgtattagg agtttttgta
    10261 gcaatagata tgattttggg aagtaattca catttaggta tgtttgtaaa agaaatttac
    10321 tttaatggac caggagaaat aatacaaaca tttagtagaa agatagagat gaatttaaag
    10381 ctagctcaaa ccagtgcatg ggtaaatata ctgttaactg gaataggtgt aattttagtt
    10441 cttatgatta atcagattag atactttaag caactgatgg atgagtatcc aattgtattt
    10501 aaaggattta tagcatcaat agcaggatgt ttagtaactc ttttagtgaa tgactctggg
    10561 attgtgtcat cagctacagc ctttatttat gtaattgtac ctatgataac attaagtata
    10621 aatctcacag cattaaaaga ataagtactg tatgaataat atgtaaatat gaacacatta
    10681 atttaacttc tttatttaca aaataaagaa gaaagtttat aaaaaataaa ttataataaa
    10741 aaagagttta cctcaaaatt gattattcta atcaaatttt aaggtaaact ttttttatta
    10801 taaagataaa taacatataa cctcatattt taggtagatt tttttcttat atgtttagga
    10861 gtgcatttaa atttttcctt aaaaattctg taaaataatt ttgtattagt aaatccatga
    10921 gcttctaata tactgcataa ggtatcattt gtgttaatta agtctttata aatatgagaa
    10981 atacgcacat catttaaata ctctaagtaa gtttgtccca tatactgttt aaattttcta
    11041 caaaaatatt ctggctgtaa gtgtgctacc ttagcgattt tgttaataga aataggcatt
    11101 ttgtaattaa tctttgtata ttctaaaact ggttcaaatt ttgacaaatc aagtactgtt
    11161 tttttcatat ttttattacc tacagatatt ttaaagtttg tatataattc aaataaaagt
    11221 tcaaataata agcttgtaaa gagcagatta gctgcaggag gagagctaag tttaatttct
    11281 ctcatgtttt ctaatataag tttaacttta cctgaatttt ttttatagtt attgtcattg
    11341 gcattaacct taaagtcaaa atagcaattt ttaaaatcag ggatatattt gtttagaaaa
    11401 gacaatggta cttgcaataa tatagaggta tcttgataag tacatctagt tgagtgaata
    11461 attccagaat taatgaacat acactcatta gcttttaaat tataagtgta accctccatg
    11521 ttaatttgta aatcaccaga agttatataa attatttcta atgagttatg ccaatgattt
    11581 gctacataac taccactatc atcaaatagt ttgaaattaa catctaaatt atcaataggt
    11641 tcaattattt catgtgaaaa attatttctg ttcataataa tatcctcctt ataattttaa
    11701 attataaata gtaaatttat aatgttcaat attaaaaaga taatatagac taataaatta
    11761 gataataaac atacttaaga aaacggataa aatcaaatat aataactgta tattaaaata
    11821 acttaaaaaa attattttat tataagtgca aaatatacct atttaaataa gataagaggg
    11881 gaggatttta tgtgtatata aaaggtgtaa atttaggagg ttggttagtt ttagaaaaat
    11941 ggatgacctc aagtttattt gaaggcacag aagctgaaga tgaatactat ttaccaagac
    12001 agctttcaag agaggcatat gaatctagaa ttaagactca tcgtagtgaa tacatcacag
    12061 agagagattt tgcaacgatt aaatctatgg gatttaattc agttcgtata ccagtaccat
    12121 actttatttt tggagattgt gaaccattca taggatgtgt taaagaactt gataaagcat
    12181 ttgcttgggc agataagtat gggcttagta tattgataga cttacataca gttccaggaa
    12241 gccaaaatgg atttgataat ggaggaatat caggtatatg tagctggtca caaaatccag
    12301 aatatgtagc gtttacatta aatgtattag aaagacttgc aaaacgttat gggatgagac
    12361 atgaattgta tggaatccaa attttaaatg agcctataac agaaagaatg tggaacataa
    12421 tgaatgttcc aaatcggttt aaggctgtag ataaggagat ggcaaggggg agtaaaccta
    12481 attcattaga gtttttacgt gatttttata taaaagcata taaggtaatg cgtccttata
    12541 tgagagagga aaacgttata gtgtttcatg atgcatttga attaaaagca tggaaagact
    12601 ttatgagaga ggaagaattt aaaaatattg ttctagatac acaccaatat ttgatgttag
    12661 ctgaatcaga tggatgtgaa caaagtattg atagctatct caaatatatt agagaaaatt
    12721 atgctaaaga tattttacaa atgcaaaaat attttcctgt tatatgtggg gagtggagct
    12781 tatttaattc atatgcttgt ggtatagata caaatggagg tcaatcacct ctaaatggga
    12841 ttgaatcaaa tatagataaa ttatcaaaag acgataaaag agaattatat aggaagatag
    12901 caaaagcaca gcttgatgca tggagaaatg gaagtggtca ctactattgg aattataagt
    12961 tattactaga tacagttaat gaagaaggtt ggataggatg ggatagttgg gacctaggca
    13021 aatgtgtagc tcaagaatgg tatcctatag aatattaaac taaaattgga ggaaaagata
    13081 tgattaaaaa ccctatactt ccaggtttta atccagatcc ttgtatttgt agaaagggag
    13141 atgattatta tcttgtagtt tcatcttttg aatggtttcc aggtatacct gtttatcact
    13201 ctaaagactt aaaaaattgg gagttatata ctcatatttt gacagatgaa acaaaaatag
    13261 atttgaagaa gcttccttca tctaaaggaa tttgggcacc ttgtttgact tattgtgaag
    13321 aagaagattt attttacata gtttatggaa ttatgaactc tatgaatgca agatattttg
    13381 atgttgataa ttatttaatt acatcaaaag atataaaggg agagtggagt gaaccagtat
    13441 atatacactc atcaggtttt gatgcgtcta ttcttcatga tgatgatggt aaaaagtgga
    13501 ttgcatcatt agactgggag actagagaag gatatgaaaa acctggtgtt atatgtttag
    13561 tggaatactg tacaaaaaag aaagaaatag taggatatcc aaagagaata tggtcagggg
    13621 gaactgatag aggttgtatt gaagcaccac atataacaaa gcgtggagat tactattata
    13681 taatgtgtgc agaaggtgga actggatatg gtcatagtgt aactatggga agggctaaaa
    13741 atatatggag accttatgaa aaagattcaa tgaatccaat tgtaacttca attccaggcg
    13801 atttttatga aagacatgac cctgaccatc taaagcctaa atactataat ccagaatcaa
    13861 aacttcaaaa atctggtcat ggaagctata tagaaacaac atcaggtgaa gtatatttag
    13921 ttcatctaac atctagacca tttgtaccag agttaaggtg tactttaggt agagaaacag
    13981 ctattcaaaa aatgaaatgg acaaaggata attggcttcg aatggaagat gaaagtaatt
    14041 tggcaaaaga atacgtatca gaaagtaaac tagaagaaca tttagtttca tctataccaa
    14101 gttttgatga ctttgattca aatgtattag gtttacagta ctatgctcct aggatttcac
    14161 cattatcatt tgcagatgtt aagtctagac caggatatgt aaggattaga ggtcaagaat
    14221 caagaacatc attaaataaa gtaagcatat tggcaagaaa attaacttca gtatatgcaa
    14281 gaataaccac taagatggag ttttacccag aggtacatca gcatagtgcg ggtttgataa
    14341 tgtattatga taatatgaat tatataaatt taagaaagta ttatagtgaa acattgggtc
    14401 aaagtgcact atcaataata catcttgaaa atggagagaa gacggaattt ttaaatacta
    14461 ggataccaat aaaagatata ccaatatatc ttcgtctgta tatacaaggt cgtaagtcat
    14521 attttgaatg gagttatgat gaaaaaaact accaaagaat aggaaaggta tttgatacaa
    14581 ctaagttttc agatgagtac tgtaaatatg gagaatttac aggtacgttt ataggattaa
    14641 cttgtgcaga tcgtgtaaag cataaacact atgctgattt tgattttttt gaatatatag
    14701 cagatgaatc aaaagatgtc gattaataaa atcattacta aaattttcag agcaaaggta
    14761 aatcttatgc tctgaaataa gaaacattag aaggcaaatc ttacaaaaag gagtatggta
    14821 ttatgaatac agaagttaat gttaaagcaa ctacgattag atatgaaaag atttcatttt
    14881 ttgaaagaat ttcttatggt tgtggggatt tggggtgcaa tatcatatac tcagctatgt
    14941 cagcattttt gctgttttat tatactaatt atgcagatgt tagtgcagca gctgtaggta
    15001 gtattatgct tgtatctagg atactggatg ggtttagtga cttaacaatg ggaattatag
    15061 tggatagaac taaatccaaa tacggtaagg ctaggccttg gattttgaga atggcgattc
    15121 cttttgctat agcagcagta ttattatttt ctgttccatc aaatcttggt atcacttcta
    15181 aattaattta tatttttatt acatataact tagtttctac tgttatttat acagcaatca
    15241 atgtacccta tgcaactcta aattcactta taactcaaaa ccagtatgaa agaggagtat
    15301 taagtatatt tagaatgata ttagccactt gtggtacctt actaataaat ggacttacac
    15361 tacctctagt tgaatacttt ggaaataatt tatcagcatg gactaaaaca ttctttgtat
    15421 ttggaatagc atctataatg gtatttttta taacatttac tggaaccaag gaaagagtaa
    15481 aagcagttaa gcaagatagg aatgaagtaa taccatttaa aataggtatt aaatctttat
    15541 ttagaaataa gtactggata caaataacat tatgtttagt ttgcatattt attgtatttg
    15601 cgataaatgg tggttcatcc gtatattatg caaagtttat tttgggagat gaaaaattat
    15661 ttggtcctat aaatatggtt tcaaatatat cccaaattat agctatgttt atggttgctc
    15721 cttttataaa aaaatttgga aaaagaaatg tattaatagt aggttcaata atattaatat
    15781 cgtcaaatgt tatgtttata atagttggac aaaactatat aggagtaata tttgcaagtg
    15841 ctataaaggg aataggaagt gctggaatag catcaacaat gtttgctatt gtttcagata
    15901 ctattgaata tggagaatgg aagactggat atagaactga ggggcttata aatagtgcat
    15961 ctagttttgg gtttaaagtg ggaaatggat taggttcagc aatattaggt gcagtattat
    16021 caattggtgg ttatgtagga acatcagcta ctcaaagtga tttagctata gtatcaataa
    16081 aagtatgttt tatataccta cctatattta taacaatatt acaaattatc ataatgtttt
    16141 tttataagct tgataaagaa tatagtacta tacttaatga attaaatact aaataatgaa
    16201 aaagtttctt ataaagtctt aagtttaatt aaggtctatt gtaaatgttc cctataaaaa
    16261 ctattatata tttatgatta ttgaagaatt tttagagaag cgaataataa aaatataaga
    16321 agactttaaa tgatgaaatg gttggtcttt tagaaatgat ttaataagtt atttttattt
    16381 aaagcattat aagtggacaa ttaattttta atttatggag atgatattta acaattagaa
    16441 gtaatatata atatgcgcta agaagcaatt tttcaactat ttatgaaggt ggtggtacaa
    16501 gaaaagtatc accttttttt tgttatacaa aaaaacagtt gactttttat atgaacccct
    16561 tctataatgt tactgaaatt aagtaaaatg attgaacaaa tgtaaagata attgaaaagc
    16621 ttgattttgg aggaaatcaa atgaaaaaaa ttggagactt aagattaatg atgaagtgtt
    16681 gttcacttta ttacgaagat aatttgaatc aacaggaaat agcaaatcaa ttgggaatat
    16741 caagaccaac aatatcaaga atattaaaag aagcttttga acaaggaata gtaaaaatac
    16801 agatagttga tgttttgaaa aatgattacc aaaaaataga gagaagtttg gagagaaaat
    16861 ataaattaaa agaagtaata gtagtagatg ataaacaaga tgcactaaca caaaaacaag
    16921 aactagcaag agctgtatct gaatatttaa ctagagtagt aaaggaaaat gatataatag
    16981 gtgtttcaat aggaacaaca ctaaaagaaa taccaagata tgtggaaaaa agtaactgta
    17041 aaaatgttac atttatacct ctattaggag ggattggaga caatgaaata gatattcatg
    17101 caaatcaaat agcagtaagt ctagcgaggg cttttggagg tgattttaaa ttattacatg
    17161 ctccagctgt tatgtctgac ttgtctacaa aagagaaact ttgcaaagac gaaaaaatta
    17221 aagaagtact agatttaata gataaaacaa ctatagcaat tgtgggtata ggaaatccta
    17281 tgagtttaaa ctcaacaata atggcttctg gatacatgaa tgaagcagat atagaagatt
    17341 taaaaaaata caactcaata ggtgcaattt gtttacaagc ctttgataaa gaaggaaaaa
    17401 cttctatact tgaatttaat caaagagtat taggagtgaa actggaagat ttaaaaaaga
    17461 taaaaagaac aataggtgtc gcttcaggag atgaaaaaat agaggcaata aaggcatcat
    17521 taaaagcgaa atttataaat agtttagcaa ttaatcacag tttagcactt aaattattag
    17581 aagattgtga ctaatagaaa gatagataaa agttaacagg agggaatgta tatgtcaata
    17641 tcaaaagaaa cattgcttga aatgtataag agaatgaatc aggctagaaa atttgaagag
    17701 aaagttagtt ggttttttgc aagaggtatg gttcatggaa caactcactt atctgtaggt
    17761 caagaagcgt caagtgtggc ggctgtaatg gctttagaaa aaggagattt agtatcatta
    17821 actcatagag gacatagtca atttatagga atgggaatag acctaaataa aatgatggct
    17881 gaacttatgg gtaaagaaac tggattttgt aagggaaaag gaggctcaat gcatatagca
    17941 gatattgagt caggtaactt aggagcaaat ggagttgtag gtggaggtct tacaatagct
    18001 ccaggagctg cacttacaca acagtataaa aaaacaggca aaatagtttt atgtagtttt
    18061 ggtgatggag catcaaatga aggtactttt catgaaggaa taaatttatc ttcaatatgg
    18121 aaattgccaa taatatttta ctgtgagaat aatttatacg gtatgtcaac aagcataaaa
    18181 cgtcatatga atatagaaag tattgccact agagcagctt cttatggaat agaaggaata
    18241 tcaatagatg gatataaccc aatagaagta tacgaaacag tacaaaaagc tgctgagaag
    18301 tgtagaagag gagaaggacc tgtattaatt gaaagtagaa cttatagatg gttaggtcat
    18361 tcaaaatcag atgcaaatgt atatagaaca aaagaagaaa tagaatcatg gaaagcaaaa
    18421 gaccctattg aatttttaaa aaattatctt atagaaaata atctatctaa tgaagatgaa
    18481 ttagataaga ttcaagaatt tgcaaagcaa tcaatagaag atgcagtaga gtttgctcaa
    18541 aatagcccaa atccaaaaat agagtcttta ttagaggatg tatatgcaga ctaagaatgg
    18601 taaaggagga aaataaaaat gagtacaagg gaattaacat atgcacaagc aataaaggaa
    18661 gcaatgtcag aagaaatgag aagagatgaa aatgtaatat ttatgggaga agatatagga
    18721 atatatggag gggcatttgg tgtatcagtt ggtatgatag atgaatttgg accagaaaga
    18781 gtaagggata ctcctatatc agaagcagct atagcaggag cggcagcagg tgcagcagca
    18841 actggtttaa gacctattat ggaagtaatg tttatggact ttgtaacaat ttctatggat
    18901 gcaattgtaa atcaagcggc aaaaatgaga tatatgtttg gaggaaaagc acaagttcca
    18961 atggttgtta gatgtccagg tggttctgga actggttcag cagagcaaca ttcacaaagt
    19021 ttagaagctt ggttttgtca tgtaccagga gtaaaagtag ttgcaccttc tacaccagca
    19081 gatgctaagg gattgttgaa agcagctata agagataata atccagtcat atttgttgag
    19141 aataaattgt tatacagaaa aaaaggtttt gtgccagaag atgattatgt aattgaaatt
    19201 ggaaaggcag atattaaaag agagggtact gatgttacag taattactta tggaagaatg
    19261 ttacaaagtg ttgaagaagc agcagagact ctatcaaaag aaaatataaa tgttgaaatt
    19321 atagatttaa gaactttata tccattagat aaagagacta tagtaaagag tgtatgtaaa
    19381 actgggagag ttttaatttg ccatgaagca gctaagactg gtgggctagg tggagaaata
    19441 tcagccctga taacagaaag tgaatcattt gattatttag atgctccagt aaaaagaatt
    19501 tgtggaaaag atgtgccaat accatataat ccagagttag aaaaagcagt tgtcccaaga
    19561 gttgatgaaa tagaagaagc aataaaatct ttaattgtta gataaggagg gatagatatg
    19621 gtggcaaata aaataaaagc cacaccagca gctagaagtc aggcaagaaa agacaatata
    19681 aaattagata ggctgattgg aagtggagaa aatggaagga ttcatttagt agatgtgtta
    19741 aattatttaa aagataataa agcaaatact actcctttag ctagaagaat agcagaagat
    19801 ttaaatatag atttagaaac tattgtaggt actggatata gtggaaagat aagaaagtgt
    19861 gatgtggaaa aattaactgc aaaagaaact attgtatcaa ataatacatc taaatatagt
    19921 gagaaaaaag agctgaaaat tgaaaatgaa aattcaagca tgtttaacac agtagaagga
    19981 atatttgaaa agccaaatcc aatgagagct actgtagcaa aacgaatgtc agaaagttat
    20041 tttagtgctc ctgtatttac attcaatata gaagtagatg ctacagaatt gaaggtactt
    20101 agagcaaagt taatcgacac agtaaaagaa tcaactggag taaaattaac tatgacagat
    20161 ttaatagtaa tggcagtatc taaaatactt ccaaatcatc aagccttaaa ttcagcatgg
    20221 acagatgagg gtatatttag atataaagat gtaaatatag ctattgcagt tggtttagat
    20281 gaaggcttat atgttccggt agtaaaaaat gcaaacaaaa agtctttaaa agaaatagca
    20341 aaagaaagca aagaacttgc ggaaaaagta aagacaggga aacttatgcc agcagaccaa
    20401 gaaggtaata catttacaat aagtaatgtt ggaatgtatg gtataactac atttacacct
    20461 ataataaata tgccttcaag tgcaatctta ggggtaggtg ctacacaaga taagtttgtt
    20521 ccagtaaatg gagaggctaa aataaaacct ataatgaact tatcattaac atcagaccat
    20581 agagtaatag atggaacagt agctgcaaaa ttcttaaaag atttaaaaga attgttagaa
    20641 aatcctttat caatgcttgt ataggttttg atatttcgtt actgatataa atagtttgaa
    20701 tagattatat tattataatt gtttcagtaa atttaaagct tttatatatt aagaattatt
    20761 tttaacagta actatatgtg aatatgattt gcttaattaa aatatatgaa aataaatagg
    20821 aggagagcct atgtcagtag aagtaataat gccaaaagca ggagtagcca tggaagaagg
    20881 tactatagta tcttggctaa agcaagaagg cgaagaagta aagattggag aacctatact
    20941 agaaataaca acagataaag ttaatatgga gatagagtca gaaggagaag gtacacttgc
    21001 tgtaatcata cataaagagg aaggtgaagt attaccagta tttacagtaa taggtgtaat
    21061 agcagaaaaa ggggaaaatc aagaggaagt aaaagctaaa tatttatctg gaaatgtttc
    21121 aaaagaagat atagttgaag agaatcaaaa tatagaagtt aaagaagaaa aaataaacaa
    21181 aaaagagtgt aatcatgatt atgatgtagt ggtaatagga ggaggacctg ggggatattt
    21241 atcagcatta aaagcagctc ttttaggtgg aagagttgct ttagttgaag aaaatatact
    21301 aggaggtact tgcttaaata gaggttgtat ccctacaaag acttatataa aaacagcaga
    21361 aatattagaa gaaattgacc aattatcaaa gagaggtgta aaagtaactg tagataaaga
    21421 acaggacatt aagaaggcca taaaatataa aaatagagta gttaaaaaac ttacagctgg
    21481 tgttggcgga ttattaaaaa gtagagatgt agatgtgttt aatcttaaag ctagtgttaa
    21541 agaagagcat aaagtaattt tatcagatgg aaaagtttta gatacagaaa atataataat
    21601 tgctacaggt tccaaagtaa ggatattacc tataaaagga atagaatcaa atttaataat
    21661 aactagtaca gaagcattag atttggaaac agtgccagaa gaattagtaa taattggtgg
    21721 tggagtgatt ggttgtgaat ttgcagagat attcaattca agaggttcta aagtaactat
    21781 agtagaaatg gaagatagag taatcccaag aatggataaa gagttaagcg aatctttaaa
    21841 atattctctt agtaaaaaag gtataaatgt attaactaaa aagaaagttt ctgaatttaa
    21901 agaagaagga aataacatat tagtgtgcat tgaaggtgaa gtgccaataa aagcagattt
    21961 atgtctatat gctataggaa gagaagctaa tctttctggc atagaggact tagatattaa
    22021 aatagataaa ggttcaatag ttgtaaatag taaaatggaa acaagtatac caagcatata
    22081 tgcagtaggt gatgttactg gaggtgtaat gttagctcat gctgctttta aaatgggaga
    22141 ggtagcagct tcaaatgctc taggtgtgaa taaagaagtt gacttaggtg cattacctag
    22201 ttgtgtttat acaattccag aagtagcatc agttggtatt acagaagagg atgctagaaa
    22261 aaaatataat gtaaaagttg gtaagtttaa ttttgctgga aatggaagag ctttagcttc
    22321 tggtcaagaa caaggatatg tgaaagtagt agcagatgct aaatatggag aaattttagg
    22381 tatacatatg tttggatgtg gtgtagcaga acttataaat catgcagcat catttaaagc
    22441 attagagata cctactgatg aagcaagtga gttgatattt ggtcatccat gtacttcaga
    22501 agctttaatg gaagctttgg cagatgtaaa tggagaatgt ttacacttac ctaaaaaata
    22561 aatctaaaca taaattatta atttaagtct attattaata ttaatttata tcataaaatt
    22621 ttaaaacact tacttagcca taccccttat tatgcaaata atttatttgt ataataaggg
    22681 gtatttttta tggaaaaata tagaatatag aatccttgta gtttttgttg tacaaaaaag
    22741 atagtaatat aaaataacat tatcatttta gaatgatttc aattgtaatt aggaaaagtg
    22801 atttttttgt t
//
LOCUS       DAEPOZ010000034        22390 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.034, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000034 DAEPOZ010000000
VERSION     DAEPOZ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22390
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.034"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(70..942)
                     /locus_tag="KR440_001077"
     CDS             complement(70..942)
                     /locus_tag="KR440_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBH0698135.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVSYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYKQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLHLNPEVFKNDALIYTNIDEVSIYNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(1336..1527)
                     /locus_tag="KR440_001078"
     CDS             complement(1336..1527)
                     /locus_tag="KR440_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698136.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            1747..2793
                     /gene="cobT"
                     /locus_tag="KR440_001079"
     CDS             1747..2793
                     /gene="cobT"
                     /locus_tag="KR440_001079"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBH0698137.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            2825..3385
                     /gene="cobU"
                     /locus_tag="KR440_001080"
     CDS             2825..3385
                     /gene="cobU"
                     /locus_tag="KR440_001080"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBH0698138.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            3395..4168
                     /gene="cobS"
                     /locus_tag="KR440_001081"
     CDS             3395..4168
                     /gene="cobS"
                     /locus_tag="KR440_001081"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBH0698139.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWIIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            4188..4802
                     /locus_tag="KR440_001082"
     CDS             4188..4802
                     /locus_tag="KR440_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBH0698140.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENDSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      4869..5048
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5178..6677
                     /locus_tag="KR440_001083"
     CDS             5178..6677
                     /locus_tag="KR440_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HBH0698141.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVGVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQMLGKK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKVKGY
                     EIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            6716..8092
                     /locus_tag="KR440_001084"
     CDS             6716..8092
                     /locus_tag="KR440_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HBH0698142.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDASVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            8093..9064
                     /locus_tag="KR440_001085"
     CDS             8093..9064
                     /locus_tag="KR440_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBH0698143.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKFGGFILWFITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYVSSFVSMLVFTLIHVLCRMILINLII"
     gene            9096..10166
                     /locus_tag="KR440_001086"
     CDS             9096..10166
                     /locus_tag="KR440_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBH0698144.1"
                     /translation="MKDLGHGANVGEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIGINPDFIIPGNGATEVIYLLMKSVKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLGKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEERKYML
                     QELRNIRNIKVYDTDANFILIRAYKKTTKELKKDLLKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            10168..11055
                     /locus_tag="KR440_001087"
     CDS             10168..11055
                     /locus_tag="KR440_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBH0698145.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSEVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVIILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKSMNDKKMIDTLCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            11043..11675
                     /locus_tag="KR440_001088"
     CDS             11043..11675
                     /locus_tag="KR440_001088"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HBH0698146.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            11684..12910
                     /locus_tag="KR440_001089"
     CDS             11684..12910
                     /locus_tag="KR440_001089"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HBH0698147.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDSLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDEGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCKERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            12907..13515
                     /locus_tag="KR440_001090"
     CDS             12907..13515
                     /locus_tag="KR440_001090"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HBH0698148.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNVVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKIPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            13505..14080
                     /locus_tag="KR440_001091"
     CDS             13505..14080
                     /locus_tag="KR440_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HBH0698149.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNIKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            14073..14825
                     /locus_tag="KR440_001092"
     CDS             14073..14825
                     /locus_tag="KR440_001092"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HBH0698150.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            14809..15930
                     /gene="cbiG"
                     /locus_tag="KR440_001093"
     CDS             14809..15930
                     /gene="cbiG"
                     /locus_tag="KR440_001093"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HBH0698151.1"
                     /translation="MNIEGNIAIICITENGKNLAVKINSLVNNCVIYQVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIIFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYVDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTKKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSIDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPIAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            15927..16652
                     /gene="cobJ"
                     /locus_tag="KR440_001094"
     CDS             15927..16652
                     /gene="cobJ"
                     /locus_tag="KR440_001094"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HBH0698152.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     GKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            16649..17401
                     /gene="cobK"
                     /locus_tag="KR440_001095"
     CDS             16649..17401
                     /gene="cobK"
                     /locus_tag="KR440_001095"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HBH0698153.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            17420..18898
                     /locus_tag="KR440_001096"
     CDS             17420..18898
                     /locus_tag="KR440_001096"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HBH0698154.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEEDGFDVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            18928..19833
                     /gene="hemC"
                     /locus_tag="KR440_001097"
     CDS             18928..19833
                     /gene="hemC"
                     /locus_tag="KR440_001097"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBH0698155.1"
                     /translation="MNIVVGTRGSTLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            19823..21322
                     /gene="cobA"
                     /locus_tag="KR440_001098"
     CDS             19823..21322
                     /gene="cobA"
                     /locus_tag="KR440_001098"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBH0698156.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEDIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDNDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEDKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            21339..22304
                     /gene="hemB"
                     /locus_tag="KR440_001099"
     CDS             21339..22304
                     /gene="hemB"
                     /locus_tag="KR440_001099"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBH0698157.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     NSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
BASE COUNT         9111 a         2212 c         4159 g         6908 t
ORIGIN      
        1 tttcataccc ttatattaac ttatataaaa atattttatt attttgggaa cgggttagct
       61 aacctttatc tagtgtatat ttatattgta agaagcagta aaaacttttc ccttactaag
      121 tttattaata aacttcttat ctttataaat tttgttagaa tttatactat cagcaagacc
      181 ataccaaggt tctatacata aaaaaggagc agtagaatta gtttcactat aataaggagt
      241 ccaaatacca actagaggga aatcttccat agatatagtt acatatttac taccatcatt
      301 attatatata gacacttcat ctatgttggt gtatatcaaa gcatcattct tgaatacttc
      361 tggatttaaa tgtaagtttt ttaattttcc aataggtatt atatcatctg taaaagaacc
      421 atttaaattg attttttcta catcattttt acttttaaac tcaaggtagt gtttagaaaa
      481 atcattttgt ttgtaaaaag gaaggttgaa tgcaggatgt ccacctatag aaaagaatat
      541 atctttagag tcagtatttt ttacaatcca ttctacctca atactattat catttaatgt
      601 atagtttatg attagctcaa aagaatatgg atacttcttt aaagtggatt cattatctgt
      661 taatttataa gatacaaatg tatctccttt atctacaatc tcaaagtcca tatctctagc
      721 aaacccatgt tgagtcatgc tatataaatt ttcttctatt attgtttcat tatcaaaaag
      781 tttacctact atagggaaca atataggtga ttgtctaccc caatattttt tattaccatc
      841 ccaaagaatg tttttgtcgt attttttaga aaatatttta gttagttcag caccaaaact
      901 gcttgattcg ataattaaat tttcattttg taatatattc atttaaaatc tcctttatta
      961 tattttatga gtatattata ctataaagta taaagattat taaattttat ttgatattat
     1021 atttgaaagg tagattttag tatattttga aaattttgtt ttgaaaattt gggattattt
     1081 aagaaatttt gtattataaa tagaaggaaa tagataaatt gtatagaata aaatatatta
     1141 agataagtta ataatgttaa atgggaagtg agtttatgga aaaaccaaat acaaaagttg
     1201 ttaactttca agagtataaa gacagaaaaa accaaagaga agtaaataga gacgaacttt
     1261 taaagcttat aaaaaaaatt gtgacttcaa aatagggagt tgttaaactc cctattttaa
     1321 tgggtttcat aataattaag acattctgag tccatctata taatctttga attgtgagaa
     1381 atctgattca ctacttaatt ctatggtttc tgaaccttcc gtataaaaat tcttaagata
     1441 gatagtataa ttattatcaa cacaatcatg ataaatacat ctatatccat cttcatacaa
     1501 actttttaac ttagaaaaat catccacaat atcatctcct taaagttctt ttaaatatat
     1561 ttttacaaaa agcatctaat tgtatgcaaa ttgacaatgt aaaataaagg aaatgaacat
     1621 aaaatgtaga ataattttta tatgtatgta aaaataaaaa gaaaaagttg aaatttttag
     1681 aaaataagag tattttttat ctaaaaagag agtttaaaaa ataaaaaaat gttgggagga
     1741 taaaaaatga gtttattaga aagtatatca aaaaacatat attccctaga taactcttct
     1801 attgaaaaaa caaagcagag attggatagg cttatacatc caactggaag cttaggaaag
     1861 atagaagata tttgcatgca attggcagga atatttggca atgaaaattt tgacacaagt
     1921 aaaaaggtta taattgcttt tgcgggagac catggtgtat atgaagaagg cgtagcacct
     1981 gaccctcaaa atataacaaa attacagttt ccaaattttt cgaaaggtct atgtggagtt
     2041 ggagtaataa gtaaatttgt aggggcagat gttgtagctg tagatgtagg aataaactgt
     2101 gatgaaaaac tggatggggt attagactac aagataagaa aagggacatc aaatatggca
     2161 aaaggtccag ctatgagtaa acaagaagcc attagatgtt tagagatagg tattgaaata
     2221 gcagagcagt gtatagaaaa agattataag gttattggta ttggggagat gggtatagca
     2281 aatactactc caagtacagc aataatatct gtaatttcag ggtgtgaccc gttagaagtt
     2341 actggtatag gtgcaggtct taagaaagaa agactaaagc ataaggctga agtaattaga
     2401 aaggctattg aaattaataa tccaaatcca actgatggag tagatatttt atctaaagta
     2461 ggaggatttg aaataggctc aatggcaggt gttatattag gttgtagtgc taataggatt
     2521 ccagtagtaa ttgatgggtt tatttcttat gcagcagcat taattgcata taagataaat
     2581 ccaaaaacta gagaatatat gatagcatct catttatcag cagaatcagg aactaaaaga
     2641 gcattagaca tattaaaatt agaccctcta ttaaatatgg atatgagatt aggtgaggga
     2701 agtggagctg ctttagcatt tgatataata gaagcttcaa actatactta caaaaatatg
     2761 gcaacttttg atgaaattga catgggaaga taaatataaa aatttaatat gtaggagtgt
     2821 tttgatgagt aaaattattc tagtaacagg aggagctagg tcagggaaga gtaactttgc
     2881 agaaaaacta tgttttgaca gaaataataa tacagcttat atagcaacct ctattccatt
     2941 tgatgatgaa atgaaagata gagtaaaaaa acatcaagag aatagaccta gaaactggaa
     3001 tacttatgaa atatatgaag atatatattc cataattaag gagatatata aagaacatga
     3061 aactgttata ctagattgtg taacattatt agtaaataat ctaatgtttg aacacaatct
     3121 aaatatagaa gagtctagtc aagaagaaat aaacaaatta gaacaataca taaaagaaca
     3181 agttgtcaaa cttattgaag aaatagaaaa aacaaatcta tactttgtat ttgtaacgaa
     3241 tgagttaggt atgggaattg ttcctgggaa taaacttagt agaatttatg ctgatattgt
     3301 aggtcgtata aatcaatata tagcttcgaa aagtaatgaa gtttattttg tagttagtgg
     3361 catagctatg aagataaagg agtagtatgc aattatgaaa agatttattt taatactaca
     3421 gtttttgaca aggataccta taaaattaaa tgtaggattt gatgatgaat tttataagtc
     3481 aatagtatat tttccacttg taggttttgt cataggtata ttatcctatt taataggatg
     3541 gatttctatg cttttatttg aaccatttat agcttctata ataataactc tagctggtgt
     3601 cttaattact ggaggacttc atatagatgg attaggagat acatttgatg caatatatag
     3661 ttatagggat aaagaaaaaa tgcttgaaat aatgaaagat tcaagattag gtacaaattc
     3721 acttctagca attatgtttg tacttttatt aaaggttgga ttcgtttacg atataataag
     3781 taataattca ttgtggatta taatttttat gccaatgatt gcaaggttag gagtaatgtt
     3841 attgacatat aaaaccgtga caccaagaga aaaaggaatg ggaaatttat ttataggaaa
     3901 attaactaca agtatgttaa taactgctat aatatacaca ttattgatag ttgctcttat
     3961 tactaaattt atatttttat tacctaatat agtgttgatt aaagtattag gttcgattat
     4021 agtggttttt gtatttataa tattattcaa aaaacatatt tataaaaaaa ttgacggagt
     4081 aacaggagat atattaggct gtggaataga actttcagag cttgtatatt taatttacat
     4141 atatttgtta atttttatgt ttttttaaag taaaaagtgg tgataatatg ataagattaa
     4201 tattaataag acatgcactg acaaatgata ataaaaaagg aagattatca ggccatataa
     4261 atagttgtat tagtgaagag ggcaaactac aaataaataa aattaccaga tatctgagta
     4321 atgaaaacat agataaggtt tatacaacac catctacacg tactaaagat acagtagaaa
     4381 aaatatcaaa attgaaatta ttagaaatag aagaaaaaga agctttaaga gaaattagtt
     4441 ttggagactt tgaaggaaga acttttgaag aaattaaaat caaatatcca aatgagtttg
     4501 aaaaaatgat aaaagaaggt aacaactata gatatccaaa tggagaaagt ttgatagact
     4561 cttataaaag agtggcaaaa gaaattgata atataatttt agaaaatgat agtaatttag
     4621 atacaaagac aatacttata tgttctcatg caggaacaat aagaaatata ataacacatt
     4681 tgatttctgg tagttataag tatcactgga actttaaaat tgataatgca tctattacag
     4741 ttttagaaat tgatggtgga tttgctgtaa tagataaaat gaattttaca gacttcatat
     4801 aatttaatca aaaccaaaca ttacaatctg tgtttgtaat atatctgtaa aattagcaat
     4861 gaaaatagaa tattagatta aaattaggtt cttaattaga tgttaaaagg gaaaaaggtt
     4921 agatgccttt gcagcccccg ctactgtgaa accaacgaaa ctataaatac cactgtcagt
     4981 ttgatgggaa ggttatagaa gtaggatgaa gttaagccag gatacctgcc taatttaatt
     5041 taaaacaaga ggtcttcggg gtgaaagatt tttaattaat atatataaaa gtcctatctt
     5101 tagaagtatt aggacttttt ttaatttgtt ttttagtaaa ttttaacaag gagggatgga
     5161 aagtggggaa aaaaataatg ctacaaggaa cagcttcaaa tgtaggtaaa agtgtcttag
     5221 tagcaggttt gtgtagaata tttaagcaag atggttatac tgttgctcct tttaagtcac
     5281 aaaatatggc tttaaattct tttataacta aagaaggatt agaaatggga agagcacaag
     5341 tatttcaagc agaggctgcg aaaatagagc caatagctga tatgaatcca gttttattaa
     5401 aaccttctgg gaataataaa agtcaagtta ttgtaagagg taaagttgta ggagaaatgc
     5461 cttcttctaa gtatcatgat tataaattgg agttagtagg tgtgttgaag gaaacattta
     5521 atagcttaaa ttcaaggtat gatattgtag ttatggaagg ggcaggaagt aatgcagaga
     5581 taaacttaaa agatagagat atctctaata tgggtatggc agaaatagct gatgcacctg
     5641 taataatagt cggagatatt gatagaggag gcgtatttgc ctcattagca ggaacaatgc
     5701 ttttattaaa tgaagatgag aaaagaagag taaaaggtgt aataatcaat aaattcagag
     5761 gaaaaaaaga gcttttacaa gatggtgtaa agatgctaga agacatcata aaagtacctg
     5821 ttataggagt agtaccttat acagatataa aaatagaaga tgaagacagt gtaactacga
     5881 gatttaagaa aaaaatgaat aaaagtgata tacacataga aataattaga accccacata
     5941 tgtcaaattt tacagatttt aatatatttg agacacaaga agatgtaagt attagatatg
     6001 tagactatgg agaatcttta ggaaatccag atatattaat aatacctgga actaaaagta
     6061 ctatagacga tttaacatat ataagaaaaa gtggtcttga atcgcaaatc aaaaatttac
     6121 atagtcaagg taaattgata tttgggatat gtggtggata ccaaatgtta ggtaaaaaat
     6181 taaaagaccc ttatcatgtt gaaagtgaaa ttgaagaagt tgatggaata ggtcttttag
     6241 atacagaaac aacgtttgag aaagaaaaga ctacaacaca agtagaggct actattgttg
     6301 aatgtatcag tgaatacatg aataacctta agggaaaaaa agtaaaaggt tatgaaattc
     6361 atatgggcat tacaaagcga ggtaatgatg taagaagttt agatttaata actaaaaaac
     6421 ttgataaaga agtgaattat actgaaggaa gtgtaaataa agaggggaat gtaattggga
     6481 cttatcttca tggaatattt gatgaaataa gctttacaag agcaatttta aacaatataa
     6541 gaaagaaaaa aggtttagga gaattagaaa gcaaagtaaa ctcttttaat gaatttaagg
     6601 ataaagagta tgataagttg gctgattttt taagagaaca tttagatata gagaaaatat
     6661 atgagattat gaaataatga tgacctttat ttataattta ggtaggaggt gtattatgaa
     6721 aaaaatttta attgctggta ctaatagtgg agtaggtaaa acaacaatat cattaggaat
     6781 aatgcaagct ttggttaaga gaaatatgaa agtacagcca tataaagtag ggccagacta
     6841 tatagaccct tcatatcaca cttttataac tggaagacat tctagaaatt tagattctta
     6901 tatgttagat gatgaaaaaa taaagtatat agtaaataag tcctcaaaag atgcagatat
     6961 ttcagtaata gaaggtgtta tgggcttata tgatggtttt ggaatagatt tagataattg
     7021 tacaagttca catacttcaa aggtattaaa gtcacctgtt atattagtta taaatggtaa
     7081 atctatggca gcatctagtg ctgctatggt acttggatat aaggaattag ataaagatgt
     7141 aaatataaag ggtgttatag taaataatgt aaagactaat agccattatc aaataattaa
     7201 gagttctata gaaaaatatt gtaatgttga agtattgggg tattttcctc caaataacaa
     7261 attctcttta gaatctcgac atctaggatt agttcctagt gtagaaatga aatcactaag
     7321 ggaaaaattt tataccttag cagatgaaat tgaacactat atagatattg acagaataat
     7381 tgaaatatca gaaagcgatg aatttgagag tgattttgat tttcaaactt tgatagaaaa
     7441 taataaaata aaagaacatg ttaataataa atcaatagct atagcatatg ataaagcatt
     7501 caatttttat tatagagaaa atatagagct tttagaggaa cttggtttag aaataaacta
     7561 ttttagccct cttgaagatg catcagtgcc aaatgcagat tatatttata ttggaggagg
     7621 ttttccagaa atatttggaa aagaattaga agcaaatgag tcaatgagaa cttccatatt
     7681 agaagcacat aacaataata tacctattta tgcagaatgt ggaggactga tgtacctagg
     7741 tgagaaacta ttgaatcaag aagaacaaga atttaatatg gttggaatat ttagtggaat
     7801 tagtaagatg acatcttctt taaagagatt tgggtactgt attggtgtag ccaaggaaaa
     7861 tactctttta tctaaaaaag gggaatctat taaagggcat gagtttcatc attcaatatt
     7921 tgaaagtgat gaaaaatgtg tatattcaat gagaaaagaa agagatggaa atatagtaga
     7981 agagtgggat ggtggttata gtaaaggaaa cactttggct acatatcttc atactcattt
     8041 ttataataac ttaaattgta tagaaaattt tttgaaaaaa ggaaatgaat aaatgagtat
     8101 tttatctatt tatataggat atgtgacaga cttaattgta ggagacccat attcttttcc
     8161 tcatccagtg agatttattg gtaaacttat aaattttact caaggtataa taagaaaggt
     8221 ctctaaaaat gataggcagt taaaatttgg gggatttata ttatggttta taaccgttgg
     8281 aataacatat ttaacaactt atgctatagt gaagctattt agttttaata tattattgag
     8341 tattgtagtg aatagtttta taatttatac aacactagcc acaaaatgtt taaaagacga
     8401 agcattaaaa atttataatg tattaaaaac cgaagatata gaaaaatcta ggattcagct
     8461 atcttatata gtaggtaggg atactactaa tttaagtgaa cctgaaataa taagagcaac
     8521 tgtagaaact gttgcagaaa atactgtaga tggtataatt gcgcctatgt tttatgcatt
     8581 tattggagga gcaccacttg ctatggccta taaagctata aatacattag actcaacagt
     8641 tggatataaa aataaaaagt ataaagatat tggttttgca tctgctaaga ttgatgatat
     8701 agccaattat ataccagcta gaatatctgt tattcttatg acaattggaa gtttcttttt
     8761 aaattataac tataggaact gttttaaaat ctctattaga gatagaaaga atcataaaag
     8821 tccaaattgt gctttttcag aaggcgcagt atctggtgct ctaggaatac aactaggagg
     8881 taccaacgtt tattttgggg aaaaagtata taagccaaca ataggggata agcttaggga
     8941 gatagataaa gaagatataa ttaaaactaa taaaattatg tatgtatctt catttgtatc
     9001 tatgttggta tttacactaa tacatgtgtt atgtaggatg attttaatta acttaattat
     9061 ataaattatt gtttgctaac ttaaggggga catagatgaa ggatttaggg catggtgcca
     9121 atgtaggtga aatggcaaga ttatatggga aaaacccaaa agaaataatt gattttagtt
     9181 caaacataaa tccaaatgta ttaccaaatt tagagagata cattttaaaa ggattagaag
     9241 agtgtaggaa ttatccagat ataaattata caaacttgag agaaaatata tcaaaatata
     9301 taggtataaa tccagacttt ataatacctg gaaatggtgc aacagaagtg atttatttgc
     9361 ttatgaagag tgttaaaaaa aaattggcta taataaatcc gactttttca gagtatagaa
     9421 gaagtgctga gttaaataat ttagatatta tagatttaga gctagattta gaaaataact
     9481 ttaaacttaa tatagatata ataaaagaaa atataaaaag atttgatagt ttatttatat
     9541 gtaatcctaa taatcctagt ggtaatgtcc aagatttaaa ggaacttgtt catttactag
     9601 gtaaacataa taaggtacta ataattgatg aaactttcat ggaatttgtt gaagatgaaa
     9661 gtaaatacag tttggttaaa tatatagaat caaataaaaa tatatttata attaaagctg
     9721 tgactaagtt ttttggtatg cctggtttaa ggctaggata tggtcttaca agcaacactg
     9781 aaattatgaa caaaatttat gaacacaaag agccatggac tattaattct tttgcagata
     9841 tactatccaa ttttattttt gaagataaag agtatattaa aaatagtaaa gaatattaca
     9901 tagaagaaag aaaatatatg ttacaagagt tgagaaacat aagaaatata aaggtctatg
     9961 atacagatgc taattttatt ttgataagag catataaaaa aacaactaaa gaattgaaaa
    10021 aagatttgct taagcaagga aacatattag ttagagatgc gtctaatttt ataggattag
    10081 atgatagttt tataagagtg gctataaaat ctcatgaaga taacaaaatt cttatagaaa
    10141 atatgaaaaa tttattgggt gattaaaatg aaatcttatg gaatatgccc agcatcttgt
    10201 ggagaatttg tacaaggtat aattgatgat gaagaatatt tatgttcata tgcaatagat
    10261 atgtattctg aagtgtatat tgaagaaaaa ttagtagata taaatttagg gagatataag
    10321 tcaaggttag caattgaaaa ggtatttgaa aaatttaatt tacctaaaaa atatactaaa
    10381 aatatttctt taaatataaa tagtaaaata cctgttggaa aaggaatggc aagttcaact
    10441 gctgatatag gagctacaat aaaggcaaca ttatcattga tagataaaga tttaagtagt
    10501 gaggagattt ctaaattagc agcagaaata gaaccaacag attctatatt tatagataaa
    10561 aatagtatat ttaatcctct aaatggaact gttataaaat acctaggaaa tctgaccaat
    10621 gcgaaagtta taatacttga accaaacaaa gtactagata ctatgaaaat tagattgagg
    10681 caagattata ataaattaaa agtagaaaat aaagaagtaa taaaaaaatc atttgctctt
    10741 ttagaagaag gattgaaaaa aaataattta tctctagttg gagaggcttg tactttgagt
    10801 agcttagcaa atgagaatat agaaaaaaaa gagtatttaa atgaaataat aaaaatatca
    10861 aaaaaatatg gagcatatgg agtaaatatt gctcacagtg gaactgtagt aggtatttta
    10921 attgataagt ctatgaatga taaaaagatg atagatacct tatgtgaatc taacataaat
    10981 tctgtctata ataaaattta tacacaaaac ataattaatg gaggaattaa gggggaaata
    11041 gaatggaata cataaagaat cctatgaaaa tagaagaaaa aagttttgaa ttgatacaag
    11101 aaataataga cgaaattaga ccagattata aatttaagaa taatattgaa gaaaaaataa
    11161 taaaaagagc tatacatact actgctgatt ttgattactt agatatatta aaaatatcag
    11221 aagaagcagt tgatagtata atagatgcat taaaaaataa tgcaagtatt tatactgata
    11281 caaatatggc tcttagtggt ataaataaaa gaaagttaga atcattagga tgtaagtata
    11341 aatgtttagt ggcagatgat gaaactgtaa aattagctaa agaaaaagga attactcgtt
    11401 ctatggcagc agtagaaatt gcagctaaag aagaaggaag aaaaatattc gttttaggta
    11461 atgctccaac agctttatat aaagttatgg agatgaaatc agaaggtaga ttagatttag
    11521 atgcagttat aggagttcct gtaggttttg taggagcaca agaatcaaaa gatgaagttg
    11581 aaaaaacaga tataccatat ataatttcta aaggtagaaa aggtggcagt aatttagcag
    11641 cagcaatagt aaatgctata ttatatacta tgtaggtacc tatatggaag aatatgtata
    11701 tatagatgga aaaaaatata gaagaggata tactacaggt tcatgtgcaa caggtgcatc
    11761 taaggctgct gtatatatgt tgattacaaa aaatagaatc aatactataa atatagatac
    11821 tcctaagggg ataccattac tactaaaagt tgataatata aatatttctg atacatttgt
    11881 ggaatgttcc ataaaaaaag atggtggaga tgatatagat gcaactcata ccatggatat
    11941 ttatgcaaga gctgaaattg tagctaaaaa tgataagaac aaagggtatc ttactctaaa
    12001 ggatatagat agtttaagca ccaatagtga gtgtaaaagt gagctctata agtttattag
    12061 ggtttatggc ggaactggta ttggtgtggt tacgaaaaaa gggcttagtg tggatgttgg
    12121 taaacctgct ataaatccga ctccacttaa aatgataaac catgaaataa gaaagcttat
    12181 aggagataat tttgaatcta tacttggaaa tgataaagtt ttaaaaataa caatctttgc
    12241 tccacaagga gaaactgtag caaaaaaaac atttaatcca agattaggta tagttggagg
    12301 aatatctata ataggtacaa ctgggattgt tgaacctatg agtgatgagg gttggaagaa
    12361 gtctctttct atagaacttc agatgaaaaa agaacaaggc ttagataaaa taatattggt
    12421 tccaggaaat catggagaac agtttataag agaaaaatta aacttagata ttaagtatgt
    12481 agttagggta agtaatttta taggctatat gattaaagaa gctcaaagaa taggatataa
    12541 aaaaatctta atggcaggtc atataggtaa atttataaaa gtatctgctg gtatttttaa
    12601 tactcatagt aaggtagcag atgctagaag tgagatatta gtggcaaact tagctttaat
    12661 gggagctaga tacgaatttt taaataaaat aaatcaatgc gtaactactg aagaagctgt
    12721 agagctaata aataatagtg aatatagaga agtatataat atattaagta ataaatgtaa
    12781 ggagagagta aagcaatact tgaatgaaga ttcagatgat attgatgtgg aagtaatcat
    12841 attttcaatg gataagtctt tattaggtaa atcggataat acagatgatt tagtggaggt
    12901 ctttatatga taaacataat aggtctagga cctggtaact tagactatat aacaaaaaaa
    12961 ggtgaaaatt taatttccac ttctgatgta ttaattggtg gtaaaagaaa tctagagtct
    13021 ataaaaaatt ttgaaggaga aaaaatagtt ctggattcta atttaagaga aattatagag
    13081 tacataaata acaataaaga aaagcaaata tcaataatag catctgggga cccattaata
    13141 tatgggatag gtagatatct atctaaaaat attgataata agatgttaaa cgtggtttct
    13201 gggattagtt cattacagta catattttca aaaatatatg tagatatgaa tgatgtatat
    13261 attacaagta gtcatggcaa aataccagat tttgattaca tattatcaca taaaaaagta
    13321 tgtatggtta cagattcaaa gataggacca aaacaaataa gtagagaaat aatagataga
    13381 aatctaaata aaattattgt tgtaggagaa aatctgtcat atgacaatga aaaaataaca
    13441 atagcgaaac cagaagagat aataagaatg gacaattttg atatgaatgt tgtggtgatt
    13501 ttagatgagg aatagtgaat ttataacagg taaagttcca attactaagg aagaggttag
    13561 agcaatatca ataagtaagt taaatttagt aaatgctaaa aacctcatag acataggggc
    13621 tggtacagga agtgtaagtg ttgaagctgc atataattat cctaatttaa aggttatatc
    13681 tatagagaaa aatgatgacg ctatagagct tatagagaaa aatataaaaa aattcaactt
    13741 aaaaaatata gaagtgataa aaggatatgc tccaattaat ctagggttaa atgttaaagt
    13801 tgagtctatt tttcttgggg gaacaggaaa taatctggaa gaaataatag aatggtctaa
    13861 aaaaaatctc gttacaggag gaagacttgt agctaatttt ataataatag atacatttaa
    13921 tcaaacttta aaactgctta aaaagcatgg atttaaagaa atagatgtat gtgttttgaa
    13981 tgtttctaaa ctagagaaac taggaaaagg tgaatatttt aaaccactaa atcctatata
    14041 tataatatct tgtgaaaaag gggaaaatga tgatgaataa agtacatttt gtaggagcag
    14101 gacctggaga taaagagctg ataacattaa aaggatacaa attattaagt aatgcagatg
    14161 tagtgatata tgcaggttca cttgttaatc ctgaactttt ggaatactgt aaagaagatt
    14221 gccaaataca taatagtgca catatggatt tacaagagat aatagatgtt atgagagaag
    14281 gtatagaaaa taataaatct gttgtaagac tgcaaacagg tgatttttct atatatggtt
    14341 caattagaga acaagtggaa gatttaaata aattgaatat agattatgac tgtactcctg
    14401 gagtaagttc ttttttaggg gcagcatcat ctttaggggt tgaatataca gttccagaaa
    14461 tttctcaaag tgtaattata acaagaatgg aaggcagaac tccggttcca gagaaagaat
    14521 caatacaatc atatgctaag catcagacat ctatggtaat atttttatct gttcaagaaa
    14581 ttgagaaagt tgtgtctaaa ttactggaag gtggatatcc aaaagatact cctatagcag
    14641 ttatatacaa agcaacttgg gcagatgaaa aaatagttaa aggaacttta agtgatatag
    14701 ctgtaaaagt taaagaaaac aacataaata agactgcttt aataatggtt gggagatttt
    14761 taggagaaga gtataataat tcaaagttat atgataagga tttcaaacat gaatatagag
    14821 ggtaatattg ctataatctg tattacagaa aatggaaaaa atttagctgt aaagataaat
    14881 tctctagtaa ataactgtgt tatatatcaa gtaaaaaata aaaaaagtaa tttagttgtt
    14941 gaagaaacat gtgtacatac agttcaaaag aagttaagtg attttgtagg agaaatattt
    15001 agcaaatttg attacataat atttataatg gctacaggta tagttgtacg aagtatagca
    15061 ccatacataa taagtaagtt tagtgaccct gcaattttgg taatggatga aaaaggcaaa
    15121 aacgtaataa gtttattgag tggtcatatt ggaggggcaa acagcttgac acagtacata
    15181 agtagtctta ttgatgctaa tccagtaata acaacagcta ctgatataaa tcaaaaagct
    15241 tccttagata tgatagcaaa aaaactaaat gcacatattg ataattttag agaaagtgta
    15301 aaagaagtaa attctatgct agtgaataat aaatgtgttg gattatatgt tgatgaagga
    15361 tatcaagttg atactagggg atttaaactt atagaaagta aggatgagtt aagagatagc
    15421 ttttcaaaca catatataga aaaattagtt gtagtaagca ataaaataaa ttgtattgaa
    15481 gatattgaga atttaaactt agataataaa tttgaacatt gctatagtac taaaaaaata
    15541 ataaaggtaa ttccaagaga tatagtgata ggaattggct gtagaagaaa cacagatagt
    15601 gagctgatgt ataattcact tgaagagttt cttaataaac aaaatataga tattagagca
    15661 ataaacacag ttggaagtat agaactgaaa aaagacgaaa aagcaataat tgaattatca
    15721 aaaatactaa aagtgccatt taaaacattt tcaatagatg aaatatctaa ggttgataat
    15781 ctttttgata aatctgattt tgttaaaaaa aatgtgggcg tgtattcagt agcagaacct
    15841 atagcatatc ttttgagcaa aggtaatttg ttagttgaaa aacataaata taaaggaata
    15901 actttttcag tggggagaat aaaaatatga tatacgttgt aggaattgga ccaggaagta
    15961 aagatacaat gactttagaa gcaataaaag caatagaaga ttctgaggct attgttggat
    16021 ataagactta tataaaacta atagaagaat ttatacaagg taaagaaata atccaaaatg
    16081 gtatgaggca agaagttgat agatgtaaac aagcagtaga ggaagcgaaa aaaggaaaaa
    16141 aagtagcagt tgtaagcagt ggtgatgctg gaatatatgg gatggcaggt ctcattttag
    16201 aattaatatc taaagaagat gaagatataa aagtaaaggt aattccaggt gttactgcaa
    16261 gtattggtgc agcagctata cttggagctc ctattatgca tgatttctgt catataagtt
    16321 tgagtgattt aatgactccg tgggaagtaa ttgaaaaaag attgagattg gctgctgaag
    16381 ccgattttgt aatttgtttg tataatccaa gaagtaaggg aagaagtgaa catctaagca
    16441 aagcatttaa aataatgggt gagtttaaag gtggagatac acctgttgga gtggtaaaag
    16501 atgtaggtag agaaaaagaa gaaaagtttg tatgtacttt tgaaaatatg gactttgaaa
    16561 aagtcgatat gactacaatg gttataatag gaaataagtc tacctatatt aatggtgaat
    16621 tgatgataac tccaagaggt tatacagtat gattttaatt ttaggaggaa cttcagatag
    16681 tttatctata tgtgataaga taaatgaact gaaaaatcaa ccatatatcc tttcagttac
    16741 tacagattat ggaagggaac ttgcacaaca acatgcagag aatgtaattt taggtaagct
    16801 tggaaaagaa gatatgctaa aatttataaa agataataat atagtaaaaa taatagatgc
    16861 cactcatcct tatgcagttg aagtatccaa aactgctatt acttgtgcaa aattattgaa
    16921 tatagattat ataaggtttg agagaaaatc tttaattgaa gaaatagatt atgataataa
    16981 atttatagta gatacaattg aagaagcctg tgaaatagca aataaaatag ggacaaatat
    17041 attcatagga acaggaagta aaaatttgaa cagatttata gaggaaataa gtgataaaaa
    17101 tttagttgtt agagttcttc caacgagtga agtcatatta agttgtgaaa aattaggttt
    17161 aagtgcagat aatatagtag ctatgaaagg tccatttagc cagtatataa atgaagaaac
    17221 atataaacat tacaatatag atttagtcat aacaaaagaa agtggaatag ctggtgggtt
    17281 cttagagaag gtaaatgcat gtaaaacttt caatatacca gtaataatta taaaaagaga
    17341 atcgattaac tacgaaaatg tatttaatga cataaataaa attaaagaca tacttgtgta
    17401 atttttagga ggagaatata tgaagaaagc cttattagta gtgagttttg gtacaagcta
    17461 tcatgaaacg agagaaaaga caatagatat atgtgaagat aaaataaaaa attcgttaaa
    17521 aagccatgat ttttttagag cttatacatc aaatatgata ataaataaaa taaaaaaaag
    17581 ggatggaata gaaatagata atccaataca ggctttagat aaaatatatg aacaaggata
    17641 tgatgaagta ataatccaaa cacttcatat tatatgtggc gaagaattta acaaattaaa
    17701 agaacaagta gatggatatt cttctaaatt caaaaagatt gtattaggaa gacctttgct
    17761 tacacatatt gaagattatc aggaagctgt agaggcaatt aagcatcaaa ttcctcatat
    17821 ggaaagtaat gaagcagtag tatttatggg acatggaaca cttcatgaat cacattcagc
    17881 gtatccagca ttggaataca tgcttaggga taatggaata aatgcttatg ttggtacagt
    17941 tgaagggtat ccagaaatag aacatgtaat aagaagatta aaagaaggaa atataaaaac
    18001 tgtaaatttg atgccattta tgttagttgc aggagaccat gctataaatg atatggcagg
    18061 agatgaagaa gattcttgga aaacaatatt agaagaagat ggatttgacg taagagttca
    18121 tttaaaagga ctaggagaaa attcttatat acaagataaa tttgttcgtc atgctgtaaa
    18181 atgtgcagag aatgctaagt tctatggtat aggagcaggg ccaggagatg gttctttact
    18241 tacgataaaa gcggttaata ctttaaagaa actggatatt ctatacactc cagaatcaaa
    18301 aaagggtgga gatagtctag ctttatcaat agttagtgaa tatttacctg aatcagtgga
    18361 aataaagtct aggcatttcc caatgagttt tgatgggaat gaaaaaagtt tagcttggga
    18421 caatgtagca caagaaatta tatcagatgt taatgatggt aaaaatgtag gatttgttac
    18481 attaggagac cctatgattt atagtacata tgtatatata atgaaaagaa tattaggaga
    18541 ggtagaagtc gaaactatac caggaatttc atcattttcc aatatagcat ctaatcagaa
    18601 ttttccatta gtcatggata aagaagcttt agtggtaatt ccttgtacta tggaagatga
    18661 taagatagag tatgctttgc aaaattataa ttcaatagta ttgatgaagg tatataaaaa
    18721 ttttaaagaa ataataaaaa aattagaaaa gtatgattta atagaacatg ctattttagt
    18781 aagtaattct tcccaagaaa gcgaaaatgt gtttacagat ttaaaagaag cgcaccttga
    18841 agataagata tcttattttt ctactatact agtaaataaa gataataaaa taaaataggt
    18901 gaaagcaata tttaaaggag acttaatatg aatatagtgg ttggaactag gggtagtact
    18961 ctagcgctta tacaaacaga gtgggtaata aatgaattaa aaaagaagta tccagaaatt
    19021 agttttgaga ttaaaatcat aaagacaaaa ggagatttaa ttcaaaatgt ttcactggat
    19081 aaaataggtg ataaaggatt gtttgtcaaa gaaatagagc aacaattatt agatggaaaa
    19141 atagacatag cagtacatag tatgaaagat atgccttctt atttagcaga tggactaaaa
    19201 tttgctcata cgcctaagag agaagaccca agagatgttc taatacttag agaaggatat
    19261 aaaaatttag atgatttgcc tcatggagca gtaataggaa caggaagtaa aagaaggaag
    19321 tttcaattat tgaaacaaag acctgattta aatatagtac aagttagagg taatgtggaa
    19381 actaggatac gaaaaataaa agatgaaaac atgcatggta ttgtgttggc agcatcagga
    19441 ataattagag ctaatttgca agataaaata agttcttatt tacctgttga tgttgttatt
    19501 ccagcacctg cccaaggagc tttagctata gaaattagaa gcaatgatag tacgatagag
    19561 ggaatagtaa attcactgaa ggatgagaat acagaaatcc aaatattggc tgaaagaggt
    19621 tttttagatg gtgtaaatgg aagttgtcat attccaatgg cagcttattg tgaaataatc
    19681 caagataaaa tacatcttac tggcttatat ggagattcag aaggaaaaaa agtagtaatc
    19741 aagtctatag atggagatat ttcttctcct agagagttag gtttaaagtt agctaaactt
    19801 gtattgaagg agtatgagaa ttatgaaggg taaggtttac ttagtagggg caggaccagg
    19861 tgattataaa ctcatgactt taaaaggttt agagtgtata agaaaatcgg atgtaatagt
    19921 atatgataga ttagcaaata gtagttattt aagagaagct aagcctgatt gtgaacttat
    19981 atatgtaggc aaggcatcaa gtaatcatat tttgccacaa gaagatataa atagaattat
    20041 agcagaaaaa gctaaagaag gaaaaattgt aactcgtctt aaaggaggag acccttatgt
    20101 atttggcaga gggggagaag aagcagagac tttagtagat gaagatatag aatttgaagt
    20161 agttccaggt ataacttctg ctataggagg tttatgttat gcgggtattc ctataacaca
    20221 tagagaccat gcttcgtcat ttcatgtaat aacaggtcat ttgaagggtg atgatagtgg
    20281 agaattaaac tggaatgctc ttgcaaataa caaaggtact cttgtgtttt tgatgggaat
    20341 ttcaaaccta gagaaaataa gtgaaaactt aatgaaagaa ggaaaagata aggatacacc
    20401 tgttgctctt ataagttggg caactagata taatcaaaga gttgttacat ctacacttga
    20461 gaatgtttat gaaactgcta ttaaagaaga agtaaaaccg ccaactctta tagtagttgg
    20521 gagtgttgta ggacttagag aaaagttgaa cttctttgaa agtaagcctt tatttggaaa
    20581 aagtattgct gtaactaggt ctagaaatca aaatagtgta ttggtagaaa aaataatgga
    20641 tttaggtgga aatcctatag aaatacctac tattaaggta gaaaaaatac aaaataatat
    20701 taacttagaa aatgaaataa aaaatataaa taagtataat tatttaatac taactagtaa
    20761 aaatgccgta gaaatcttct ttgaaaagat atatgaaatg aattttgatt taaggatatt
    20821 atctaattta aaggtatgtg ctataggttc tgctacatca aatgagttga aaaaaagagg
    20881 aattatagcg gatatagtac caaaaaaatt tgtagcagag tcactgtatg aagagttagc
    20941 tcctatttta aatgataacg ataatatact tatacctaga gcagaaaatg ctagagatta
    21001 tctggttgac aaacttaagg ctatttgtga agtaacagaa gtacatatat atagaacagt
    21061 aatagatgag gataaaaaag gtgagatatt agatatttta aattctaatg atatagatta
    21121 tataacattt actagttcct caacagtaaa taatcttgtt gaaattatag gaaaagaaaa
    21181 tattaaaata ttagaaaaaa taaaaacact atctataggt cctataacta cagagacaat
    21241 aaatagtttg ggaataaaat tgtacaaaga atctgctaca tctactatag attcgttagt
    21301 agatttaatt ataaaaaatt aattttggag gcttgaatat gttaagaaga ccaagaagac
    21361 taagagcaag taaagcaatc agaaatttgg taagagaaac taaggtgaat gtagaagatt
    21421 taatatatcc actgtttgta gtagaggggg aaaatataaa agaagaaatc aattctttac
    21481 cagatgtata tcatttatca atagacatgc tagaagacga aataaaagaa ataagtgagt
    21541 tgggaattga acatataatg ttatttggtg ttccagatga aaaagatgag tgtgcttcac
    21601 aagcttttaa tgataatgga atagttcaaa aggcaattag aaaggtaaaa gaaataaatt
    21661 cagatataaa tgtaataaca gatgtctgta tgtgccaata tacaagtcat ggacattgtg
    21721 ggattttaac tgatactggt tatgttgata atgataaatc attggagtat ttggctaaaa
    21781 tatctgtaag ccatgctaaa gcaggagctg atatggttgc tccatctgat atgatggatg
    21841 gaagaataca agcaataaga gaagctttag atgaaaatgg attagaaaat gttggaatca
    21901 tggcttatag tgttaagtat gcttcaaatt attatggacc ttttagagag gcagctcatt
    21961 cagcaccatc ttttggagat agaaaaacat atcaaatgga cccagcaaat agcaatgaag
    22021 ctataataga gactgaactt gatatattgg aaggtgcaga tatacttatg gttaaacctg
    22081 cattatcata tttagatatt ataagaagag ttaaagataa ttttgactta cctctagcag
    22141 cttataatgt aagtggagaa tactctatgc ttaagtttgc tgtgaaggaa ggtttattaa
    22201 atgaagatgt aattcttgaa tcaattacat ctataaaaag agcaggagca gatataataa
    22261 taacttattt tgctaaagac attgctaaac tgttgaaaaa ataaattcta gtagtataaa
    22321 gaatatcaaa aagtatagga attgtaatta actattatac agtagttttg tttagacatt
    22381 tattgaagaa
//
LOCUS       DAEPOZ010000035        22240 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.035, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000035 DAEPOZ010000000
VERSION     DAEPOZ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22240
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.035"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..1395
                     /locus_tag="KR440_001100"
     CDS             <1..1395
                     /locus_tag="KR440_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBH0698158.1"
                     /translation="ELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKMDTTEI
                     LGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSKIITNR
                     DCHQSVINGCILGDIDIEYVPCEISEETNVLKGISISNVIDIIDKNLDAKAVLLTYPT
                     YYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQSTHKTL
                     PAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGKELMEE
                     LLNNIRIFKDKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQVELSNY
                     YGVLLICTIGNTAEDFSSLETALNNVVKEDFKFEKLENKKYPVVIPKKILTPREAFYK
                     TKKSVKIYDSIGKICGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIVSGLKD
                     VDLNFIQVIDLEDI"
     gene            1526..2215
                     /locus_tag="KR440_001101"
     CDS             1526..2215
                     /locus_tag="KR440_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBH0698159.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2218..3153
                     /locus_tag="KR440_001102"
     CDS             2218..3153
                     /locus_tag="KR440_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBH0698160.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3150..4043
                     /locus_tag="KR440_001103"
     CDS             3150..4043
                     /locus_tag="KR440_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBH0698161.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4121..4867
                     /locus_tag="KR440_001104"
     CDS             4121..4867
                     /locus_tag="KR440_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBH0698162.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRASSRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            4868..5701
                     /gene="rsmI"
                     /locus_tag="KR440_001105"
     CDS             4868..5701
                     /gene="rsmI"
                     /locus_tag="KR440_001105"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBH0698163.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRTLKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            5724..6428
                     /locus_tag="KR440_001106"
     CDS             5724..6428
                     /locus_tag="KR440_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBH0698164.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLNTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(6616..7044)
                     /locus_tag="KR440_001107"
     CDS             complement(6616..7044)
                     /locus_tag="KR440_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBH0698165.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7081..8511)
                     /locus_tag="KR440_001108"
     CDS             complement(7081..8511)
                     /locus_tag="KR440_001108"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBH0698166.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            8643..9227
                     /locus_tag="KR440_001109"
     CDS             8643..9227
                     /locus_tag="KR440_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HBH0698167.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9243..9764
                     /locus_tag="KR440_001110"
     CDS             9243..9764
                     /locus_tag="KR440_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HBH0698168.1"
                     /translation="MEIFSNLLIPIIILYIVVYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    9830..10076
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10160..12097
                     /gene="metG"
                     /locus_tag="KR440_001111"
     CDS             10160..12097
                     /gene="metG"
                     /locus_tag="KR440_001111"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBH0698169.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTDFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12127..12897
                     /locus_tag="KR440_001112"
     CDS             12127..12897
                     /locus_tag="KR440_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBH0698170.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGICYEEVCRVTKENAKKFFNIK"
     gene            13234..13665
                     /gene="phnG"
                     /locus_tag="KR440_001113"
     CDS             13234..13665
                     /gene="phnG"
                     /locus_tag="KR440_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HBH0698171.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            13680..14255
                     /gene="phnH"
                     /locus_tag="KR440_001114"
     CDS             13680..14255
                     /gene="phnH"
                     /locus_tag="KR440_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HBH0698172.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTKLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTVSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSTINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14258..15343
                     /locus_tag="KR440_001115"
     CDS             14258..15343
                     /locus_tag="KR440_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HBH0698173.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRDFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRSAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKVKHPIYEDEEIIIGDMLVTECEIVSKASQNK
                     TENSKTNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIK"
     gene            15363..16232
                     /locus_tag="KR440_001116"
     CDS             15363..16232
                     /locus_tag="KR440_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HBH0698174.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16225..17091
                     /locus_tag="KR440_001117"
     CDS             16225..17091
                     /locus_tag="KR440_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0698175.1"
                     /translation="MSKINVSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQRELKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17132..17845
                     /locus_tag="KR440_001118"
     CDS             17132..17845
                     /locus_tag="KR440_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0698176.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMAKGTISESGVA"
     gene            17846..19015
                     /locus_tag="KR440_001119"
     CDS             17846..19015
                     /locus_tag="KR440_001119"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBH0698177.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDRIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVLNTIVNGKTVYKREV"
     gene            19019..19855
                     /locus_tag="KR440_001120"
     CDS             19019..19855
                     /locus_tag="KR440_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBH0698178.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSSLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            19856..20851
                     /locus_tag="KR440_001121"
     CDS             19856..20851
                     /locus_tag="KR440_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0698179.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLDVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            20869..>22240
                     /locus_tag="KR440_001122"
     CDS             20869..>22240
                     /locus_tag="KR440_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBH0698180.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     DFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYIIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAK"
BASE COUNT         8887 a         2482 c         3973 g         6898 t
ORIGIN      
        1 gaattaaata agattgtaaa tgaagaagta atatcttttc atatgcctgg tcataaaaag
       61 ggacaggttt ataaaaagtt aggatataaa gatatactaa aaaatttgta taaaatggat
      121 acaactgaga tattaggaac agataattta cactttccgg aaggaattat aaaaaattca
      181 caaaaaaaag catcaagagt atttaaaagt gattatactt gctatttagt aaatggtagt
      241 agctgtggaa tacaatctgc aataatgtca gtatgtagtc ctaaaagtaa aataataaca
      301 aatagagact gtcatcagtc tgttataaat ggatgtattt taggagatat agatatagag
      361 tatgtgcctt gtgagataag tgaagagaca aatgtcttaa aagggatatc tataagtaat
      421 gtgattgata taatagataa aaacttggat gcaaaagcag tactgcttac ttatcctaca
      481 tattatggaa tgacatatga tttagaatac atatgtaatt atgcacatag caaaaagatg
      541 gtagtgatag tagatgaagc acatggtgcg catttagggc ttagtgagag gcttcctaaa
      601 acagctcttg agcaaggtgc agatatagtt gtacagagta cacataaaac tcttccagca
      661 tttacacaat cgtcaatgat acatatacag ggagaccgag ttgatataga taaaatattg
      721 aacatgttaa gaattacaga atcttcaagc ccttcatatt tattattatc ttctctggaa
      781 ttagcagtag atatatatga aaataaagga aaagaattaa tggaagagct tttaaataat
      841 attagaattt tcaaagataa agttgattca aatattaata tatatgacac aaatgataaa
      901 acaaaaatat ttatatcatc taaaaacatg ggtttaacag gttatgaact agaaaatatt
      961 ttgagaaaca aatataacat acaagtggag ttatctaatt attatggagt tcttttaata
     1021 tgtacaatag gaaatactgc tgaggatttc tctagtttag aaacagcatt aaacaatgtt
     1081 gtaaaggaag actttaaatt tgaaaagtta gaaaataaaa aataccctgt tgttattcca
     1141 aagaaaatat taacacctag agaagcattt tataaaacta aaaaaagtgt taaaatatat
     1201 gacagcatag gtaaaatttg tggtgaatgt atagttccat acccacctgg aataagtgta
     1261 atatctcctg gtgaagtaat aagtaaagaa ataatagact atctaatgtt ttgtcattct
     1321 aaaggcatga ttgtgagtgg attgaaagat gtagatttaa attttataca agtaatagat
     1381 ttagaagaca tttaaaaatt atgatataaa agtagataga agtagatagt ttaaaattgt
     1441 gaagagaaat aaatagactg cttatataga gctatctaat aaaagtacta aaaaagtaaa
     1501 aagtgtttgt atggaggata tgaaaatgaa aggtaagtta ataattatag agagtggttc
     1561 agatgcaagt ggaaaggcta ctcagacaga aatattatat gaaagattaa aaaaagaagg
     1621 ctatatgatt aaaaaggtag aatatccaaa ttatgaatct ccatcatctg ccttagtgaa
     1681 aatgtaccta aaaggagact ttggtaaaaa tgcatcagat gtagacccat atatagcatc
     1741 aacttttttt acagctgata ggtatgcatc atttaaaacc gattgggagg agttttacaa
     1801 tgaaggtgga ataattatcg cagatagata tacaacctct aatatggtac atcaggcctc
     1861 taaaatggat atttgtgata gagaagagta tttaaattgg ttagctgatt atgaatttaa
     1921 tttatttaag ataccacaac ctgattgtgt tatattttta gatgtgccta ttgaatttag
     1981 taaaaagctt atggagaata gaaaaaataa aattacaggg gaagaaaaaa aggatattca
     2041 tgaaagtgac atagagtatt tagagaaatc ttacaacaat gctttatata tagcagataa
     2101 atatgattgg aaaaaaataa attgtgtttt agataacaaa ttaagaagta ttgaaagtat
     2161 acataatgaa atttataaaa tagttcttga ttcaataaaa cctatggaga gataaatatg
     2221 tattttgaaa atattatagg gcaagatttt gcaaagaaat atttaacaaa ttctataaag
     2281 aaaaataaac ttaataatgc gtatatgttt gaaggaatgg atggaatagg aaaaaagaaa
     2341 tttgcagatg aattatcaaa actattactt gattatgaaa atctcgaaaa tagtccagac
     2401 tatgtactca taaaaccaga tggaaatagt ataaaaatag ctcaaatcag aaatttacaa
     2461 tcagatatag taataagacc tcataaagac tataaaatat atataatcaa taatgctgaa
     2521 aaaatgactg tggaagctca aaatgcactt ttaaagacgc tagaggaacc tccaaactat
     2581 gctataataa tcttagtcac aaacaacaag gaatctttat tagaaactat aaaatctaga
     2641 tgtgatataa taaagttctc acctatacca attgaagact taaaacgtta tttgataaat
     2701 acaggtatag aagaagaaag agctcaatta ttggctattt tttcgagagg aagtattgag
     2761 aatgctctaa atctatctca gtcttctgag ttttcaatga tgagagaaga tatacaacag
     2821 tatattcaga taatgttaga taaaaacata gtggagattt taaatatacc aaacaatatg
     2881 gaaaaatata gaggtaagat tatagcttta ttagatatga tgataaacta ttttcgagat
     2941 atcatcctat taaaagaaaa tgtaaacaag aacatgttaa taaatgtaga taagctagtt
     3001 ttcattcaaa atatgagtgg aaaaattagt tattctcaat tgtccaagat tattgatata
     3061 atagaagatg caaagagtaa aataaaaagc aattgcaatt tcaatataag catacaagtt
     3121 atgtctttaa atatatacga ggtgattaaa tgataaagat agtaggtata aggtttaaaa
     3181 gtgctgggaa aatatattat tttgaccctg ttgattttaa tatagagcaa gatatggatg
     3241 tagttgtaga aactgcaaga ggcttggagt atgggaaagt agtcgttggt ccaaaagata
     3301 tggatgaaag cgaattagtt tctcctttga agccaattat aagaatagct acagaagaag
     3361 ataaaaaagt atatctagaa aataaggaaa aggcaaaaga aacttttgag ttgtgccaac
     3421 aaaaaataaa agaacatgag ttaacaatgt ttttaataga ttgtgagtat acgtttgata
     3481 ggaataagtt gattttttac tttacagcag aaggaagaat agattttaga gaattggtaa
     3541 aagacttagc tgctatattt aaaactagaa tagaacttag acaaataggt gtcagagatg
     3601 aagctaaatc tataggaggt ctagggcctt gtggtagaaa actttgttgt tcatcttggt
     3661 taggtgattt tcaaccagtt tcaataaaaa tggcaaaaga ccaaagtcta tctctaaatc
     3721 caacaaaaat atctggaata tgtgggagat tattctgttg cttaaaatat gagcatgatg
     3781 tatatagtga agcaatagaa aaaatgcctg ttgtaggtgc attggtgcaa tcagatgatg
     3841 gaaaaggaaa agttgtagaa gtgaatcctt tattagaaca aattaaagtg gaatttcaag
     3901 acaagacagt aaagatatac acaagagaag atataaaaat acttagagag cctaaaaaat
     3961 gtgatggctg tggtggtaaa tgtaaggata atgatggatt agatgaagcc acactaagag
     4021 agctaaagaa attagaagat taaatttaag gtagggcttt tcctaccttt tttaaagcaa
     4081 ttaatgctta aagttattaa tatttgagga gaatttatat atgcaattaa aagattcaga
     4141 aagaatagat gatttacaat taaaaggcct taaaattata caagatacta atggtttttg
     4201 ttttggtata gatgcagttt tattagccaa ttttactaaa ataaaaaaag atgccaaagt
     4261 agtagatttg ggtactggaa ctggaattat acctattttg atagctggaa aaagtgaagc
     4321 taaaaaaatt attggcgtgg aaatacaaga agatgtatat gaaatggcaa ctcgttctat
     4381 aaaattaaat ggattagaag aaagagttga gattataaat gaagatataa aatacataga
     4441 caaagtctta gacataaatg gttaccatgt agtcacatca aatcctccat atatgcatat
     4501 tgatggaata aaaaatccaa atgataaaaa agcaatatca agacatgaag taaggtgcaa
     4561 tttggaagat gtaattagag cttcatccag attggttatg cctagaggaa aattcttcat
     4621 gatacataga ccaacaagat tggttgatat aataacttta gggagaaaat ataaattaga
     4681 accaaaggtt attcaatttg tgcatccaag acctcaaaaa gcaccgaact tagttttggt
     4741 tcaatttgta aaagatggaa gaccagagct taaaatattg gacccattgt atgtttatgg
     4801 agaagatggg aattatacaa aagaattaaa agcaatatat aataataaag atataggaga
     4861 gttgtaaatg agtgggaaat tgtacatatg tccaacacca ataggaaacc tagaagatat
     4921 aacatataga actttaagaa cattaaaaga agtagacttg attgcagcag aagatacaag
     4981 acatagtata aaacttttaa atcattttga aatctcaaaa ccactaacta gttatcatga
     5041 acataataag gattcaaagg gagattattt gataaataaa ctaattgatg gtgaaaatat
     5101 agctctgata agtgatgcag gtatgcctgg tatatctgac ccaggagaag agataataaa
     5161 acaagctata caaaataaca tagagataga agtattgcca ggtgcaacag cctttgtaac
     5221 ggctttagtt ggctctggta tggacacaca tagatttgtt tttgagggct ttttagatag
     5281 agataaaaaa gttaggaaag cacaattgga agaattaaag gaagaaagta gaactataat
     5341 tttctatgaa tcaccacata gattgaagga tacactaaaa gatatgctta aaattcttgg
     5401 taataggaat atttctataa atagagaact tacaaaaaaa tatcaagaaa taataagaga
     5461 aaatatagaa actaccataa aaatatttga ggaaaaagaa gtaagaggag agtttgtatt
     5521 aatagtagag ggttttcatg gtgagaagtc aaagaaagaa aattatgatg ggcttacaga
     5581 tagggaatat gttttaaaac taattgaaag tggtatgaac aaaaaagatg caataaaaat
     5641 agtttgtaaa gatagaaagt taaagaaaga tgtggtttat aaacaagttt tagatttata
     5701 aaacctgaat ctgaggtgat tttatggaaa tagggaaaat tataatagag agtttaaaga
     5761 gggaagatac acataaaata tttatagaat tagataataa gcatgaatta gaaaaaataa
     5821 ttcctaaaat aagctgtatg aagtctgtag gtgaatgtaa atatcatgta gtaaattgtt
     5881 ttgaacattc tataaatgca ttaaaagaat tagaaatagt attaaatgat aaggattttt
     5941 ttccaataca tctaagaaaa catgtgtcaa actatttaaa cacatatatt gaagaaggaa
     6001 taaataagtt acatgtatta aagttgggaa catttttaca tgatataggt aagcctgata
     6061 gtatgacctt agatgaaacc ggaagagttc atttcacaaa tcatgaaaaa ataggtgctc
     6121 aaataattga caatatggga ataaagttag agttatcaac agagacatat agacttataa
     6181 gtaaatatgt aagatatcat atgatacttt tgtctctata taagaaaaat gatttgagta
     6241 ggaaagaatt aattaatgta tttaacttag tagatgagga cactataggt gtaataatgc
     6301 ttgggtatgc agatatagtg tctacagtaa aattattggg caagagtgga gaagtcagtg
     6361 tgttaaagac atatatggaa tatattttaa caaattactt gtataagagt aattatacac
     6421 atgtataaat ttagttaata taattgagtt ttagatgatt aattgaagtt tgaaatatac
     6481 ggatttcaaa aacataaaag aagttgtctt tgttgtaaaa ttatgaaaga caactttttt
     6541 tatatcaaaa attgaaattt tattaaggat acttaatggt ctattaatta ataaaccatt
     6601 agtatttaat ttatattaca atattgattt tgcttgtatt ggcgttgata gtataacaga
     6661 ggtcttagta gaaccaattt tctttatagt atcaatcaca ttttctagct cgtacatatc
     6721 ttttacaatt acttttaaaa gtaagcaatc atcacctgtt atatggtgac attctacaat
     6781 cctagggtcc tttgcagctg actcaataaa ttctgtatat ccattgcttg gaagagaaat
     6841 atgaataaat gcctttataa ctctgcctaa tgaatctggg ttgacaatag ctttatatcc
     6901 ttctataaca ccagactctt ctaatctttt gactctttct gaaactgcag gagaagttaa
     6961 accaactatt tttcctaagt ccttcataga aattctccca tcatcctgta aaatttctat
     7021 gattctgtaa tctgtaacat ccataaataa acagtccccc ttaaataaaa ataattaata
     7081 ctataaaact tttttagaat ctatcatttg tgtttttaaa taccaaagat ttctagataa
     7141 atcaacctta taaatgtgtg gttttgaaag tcttttgtat tgttcccaga tagtacttaa
     7201 ttcatattgt acatgatttt ttatttcaag aacagctttt ttattatatt tgcatctgcc
     7261 ttttatatac aatggtttaa gaagctcttt aactttgtaa tttgtaaata cctttgtttt
     7321 ccatgtataa gtaggatgga agatttctaa aggtttagac tcatctataa cttcatcatg
     7381 taacataatc aaatctgcct ctgccttatt gtcttcgtta tatattctta taactttctt
     7441 gtaaccagga ttatttattt tttctggatt ctcagaaatc tttatcttag gttcaagaac
     7501 tcctttttca caagatgcag ctaatttata aactcctcca agagaaggtg aatcgaaaga
     7561 tgtaatgagc tttgtaccta ctccccatga atttatagta gctccttctg ctttaagtga
     7621 tgttatggta tattcgtcta ggtcattaga tgcagtgatg ctgatatctg aaaagccagc
     7681 gttatctaat tgttttttag cttctttaga taaataagct aaatctccag agtcaagtct
     7741 tatacccata ggtttgtatc ctttttcaga aatatttttg aatactttta ttgcattagg
     7801 aactccactg tttaatacat cataagtatc tacaagtagt aggcatttat ctggataaac
     7861 atcagcatat gcttggaatg cttcaagttc attatcaaat ttttgtaccc aactgtgagc
     7921 ttgtgtacca attataggta tatcaaacat tttccctgct agtacgtttg cagttccagc
     7981 acaaccacca actacagcag ccctagctcc atatatccct gcatctggac cttgtgcacg
     8041 acgtaatcca aattcaaata cagggtctcc ttgagctgca aagcaaactc tagaggcttt
     8101 agtagcaata agagactgaa aattaactat tgttaacaat gctgtttcaa taagttgagc
     8161 ttgatataaa ggtgccttaa cagtaattaa tggctcatta gggaacatga tagttccttc
     8221 ctcaacagca tatatatctc ctgtgaattt aaattctttt aaaaatttta aaaacttgtc
     8281 agaaaataaa tttaagtttt ttaagtactc taaatcttca tctgaaaaat gaagattatc
     8341 tatgtattca acaacctgat caataccaca aactatagta taccctccat cacaagcgtt
     8401 ttttctaaaa aacatatcaa atacaacaat gtcttcatga atattctttt caaaatagcc
     8461 atttaacata gtaagctgat ataaatcagt aagaagtgtt agatttctca ttatttctct
     8521 ccttaattat tattaaatat taaaaattgt aaaaactccc acacttatac taaaacataa
     8581 tatagtacaa ctttcaagta taaagtatta cctaactatc ataaaattat ttaaagagga
     8641 ggatgattat aataggaaaa atatggtttt atatggtatc gataggaata ataggaagct
     8701 tgatttctaa aaatatgggt gaattaaata aagttatatt aagtgaaaca tcaaaaggta
     8761 tagagtttgc aataagtcta gctggtatca tggcactctg gatggggata atgaatatcg
     8821 caaaagaatc aggtttaatt gaaaagatag gtcaaaaact aaatcctata atgagaagat
     8881 tatttccttc tattccaaag ggtcataaag caatgtctta tatagttatg aatattgctt
     8941 taaatatgtt gggtgctgga aatggtgcaa cagcatttgg tttaaaagca atgagtgagc
     9001 ttcaaacttt gaataacaag aaagatacag ccactaatga tatgattatg tttatggtaa
     9061 tcaatatatc atctatacaa ataattccat ttactataat caaattaagg atggatatgg
     9121 gggctcaaaa tccaagtgaa ataattttta cgactttatt tgcaaccata atatctacag
     9181 cagtagcaat aataacatgt aaattttttc aaagaagata tagataaagg ggatgtagat
     9241 acatggagat attttcaaac ttacttatac caataataat tctgtacata gtagtttatg
     9301 gtaaatataa aaaaatagat gtgtatgata gttttgtaaa aggagcaatt gatggattaa
     9361 aagcagcatg ggatatccta ccatatataa taggtatatt tttggcaata ggaatattca
     9421 aaactggtaa aggtcttgat atgttagaat ggctatttac accaatagca aatatgatga
     9481 gtataccaaa agaattaata ggtctaataa gtgtaaaacc tttatctgga agtggtgctt
     9541 tagggatgta cagtgaactt gcaaatagag ttggaatagg aagtttagta gaaaaaatgg
     9601 gtgctacgat tgtaggttct tctgagacta ttttttacac aatggcaata tactatggaa
     9661 gtctgaagat aaaaaatact agacatacat tatcatgtgc tatgattagc catgttgcag
     9721 gtgtcatagc agctgttttt atttgctatg taatatttgt ataatttgtt gacaatatgg
     9781 gaaaaatttt ataatatagt gattatatag aaacaaatag taatacaaat tacaagctat
     9841 gaaaagaaga gtaaataaag gttttttcta tcagagagct aggttaggtg aaagctagtg
     9901 tcaaaagctt tattgaagat ggtcttggag ctttttatct gagcaagtgt gctagggtaa
     9961 aacggttgga cacgttatag tctactaagg tatctataaa atgtttctac atttcatgtg
    10021 aaacataact attatagatg aattagggtg gtaccgtgaa taatctcgcc cctatgttaa
    10081 ctcataggag gcgatttttt attatttaaa tgaaagaatc atatgattac gaacgataaa
    10141 aaattaggag gtaaataaca tgagcaaacc gagtttttat gtaacaacac caatatacta
    10201 tccaagtgga ggattacaca taggacatac ttattcaaca gtagctgcag atacaatagc
    10261 tagattcaag cgtttctgtg gatatgatgt aaagttctta acaggaacag atgagcatgg
    10321 tgaaaaaatt caaaagaaag ccatagaaca aggaatgtca gagatagagt atcttgatgg
    10381 aatgataaaa gatataaagg ccttatggaa tactatggat atatcttatg atgattttat
    10441 aagaactaca gaaaaaagac atacagacat aatacaaaag atatttacta aactatatga
    10501 gcaaggtgat atatataaag gtgaatatga aggtagatac tgtactcctt gtgagagttt
    10561 ctggacagaa tctcaattac tagaaggaaa taaatgccct gactgtggaa gagaaacata
    10621 tttagtaaaa gaagaatctt atttctttag attatctaaa tatgaagaca gattaaaaga
    10681 gttatttaaa gatgatagtt tctgttttcc tgctgctaga aaaaatgaaa tggtggcaaa
    10741 ctttttagac aaaggattag aagatttaag cgtaactaga acgacttttg actggggtat
    10801 aaaagttcct tttgatgaaa aacatgtaat ttatgtgtgg gtagatgctt tatgtaacta
    10861 tataacagca ttaggttata tgactgataa tgatgaagaa tttaaaaaat attggcctgc
    10921 caatgttcaa atagtaggta aagaaatagt gagattccat acaataatat ggccagcgct
    10981 tttaatggct ttaggtctag aagttccaaa acaagtattt ggacatggat ggatactatt
    11041 tgctgatgat aaaatgagta aatcaaaagg taatgtggtt tatccagaac ctataataga
    11101 aagatatgga atagacacac ttaagtactt cttattaaga gagtttgcat ttgggcaaga
    11161 tggaagttat actcatagaa attttgtaac gagaataaat tatgatttag caaatgattt
    11221 aggaaactta ataagtagaa ctgtagctat ggttgaaaaa tacaataatg gtataatacc
    11281 aacagcaaaa gtttcaactg attttgatgc tgacttaaaa gaacaagctg tttcaactag
    11341 agaaaacttt gaagctgaaa tggataaaat gcaattccat gaagcattag agagtgtatg
    11401 gaaattagtt agaagaacta acaaatatat agatgaaact atgccttggg cactagctaa
    11461 agatgaaacg aagaaagatg aattagacac agttttatat aatttatgtg aatctataag
    11521 aataatcgca acactaataa atcctataat gaatgagact gctaataaaa tatatgaaca
    11581 tataggaata aaaggtcaag atgatataac tacatgggaa agtacaaaaa catttggtct
    11641 tattggagaa aatgtaaaag tatttaaagg tgaaccttta ttcccaagat tagatgtaga
    11701 aaaagagata gaagaattaa ctaaaatgtt ctctgggaaa cctccagtag aagaaaaacc
    11761 tttagagcat aaagaagaaa ttactattga tgatttagac aaaatagagc ttagagtagg
    11821 gaaaataata agctgtgaaa aacatccaaa agcaaataaa ttattagtat cacaagttaa
    11881 gattggacca gaaacaagac aaatagtatc aggaatagct gaatactata agccagagga
    11941 tttagtagga aaagaggtta cagtggtatg taacttaaaa ccagttaaat taagaggtgt
    12001 agaatctcaa ggtatgatat tagcagcagg tgatgatgga gacccatatg ttttaccatt
    12061 tacacaaggg gctaaagatg gatgcgaggt tcgttagtag gtaattaaat agtaaggaga
    12121 ttaaagatgt tatttgactc acatgcacat ttaaatgatg aaagttttga tgaagataga
    12181 gatgaactta ttggttcatt aaaagataaa ggtgtagatt tggttgtaaa tccaggagca
    12241 gatatagaga cgtcgataac agcaattgaa cttgcgaaaa aatacgactt tatatattca
    12301 gcagttgggg tacatccaca tgatgtatca aaactagatg atacagcaat agaaactcta
    12361 agaaaacttg caactgagaa tgaaaaagtt gtagctattg gagagattgg attagattat
    12421 tattatgatt attctccaag agaagaacaa aaagagtggt ttaagaagca aattgaatta
    12481 gctaatgagc taaaacttcc aataataata catgatagag atgcacatgg agatactttt
    12541 gagataataa agaatactaa aaatcctgaa ataggatgtg tacttcattg ttatagtgga
    12601 aatgtagagt tagctagaga atatgttaag atgggttgtt atatatcaat tccaggcacg
    12661 gttactttta agaataataa aaaaactaga gaagtagtaa gagaaatacc tctagaaaga
    12721 ttattcatag agactgattc accatatatg tcaccagagc cacatagagg aaagagaaac
    12781 aatccttctc aagtatcttt tgttgctgat aagatagccc aggaaaaagg gatatgttat
    12841 gaagaagtat gtagagttac aaaagaaaat gcaaagaaat tttttaatat aaaatagaca
    12901 aattaaatct tataatgata tatataataa gtcattataa gtataggatg agagtgcctc
    12961 aaaatgagtt ttttagttca tgaggcactc tttttttatg aaatcaaata tattttcatc
    13021 atttcaacaa tgaaaaagaa gtttatcata ttttgagaca gtttgttttt atttgtttaa
    13081 ttcatatttt aaaatatatt ttcttaacat tgttaaaaaa gaatttacaa gatattcatt
    13141 agaattaact caactctaat attcacttaa cttaagtggt ttattatatt attaaagaga
    13201 agtaaacgaa aggtagatta aggaggctaa attttgtata cagttgatag tactaagata
    13261 ttgataaata tgaacttaga gaaactagaa aagatatact caatgataaa agatacatca
    13321 agtatttcta taattaaaga acctaacttg gctacagtaa tggtaagagc taatgaaagt
    13381 gtaaaaaata caacatttaa tttaggagag atattagtta ctgaatgtag tgtaaaggta
    13441 gatgaaagct tgggttacgg aatagtttca gaaaacaata ataagaaagc catttactta
    13501 gcagttatag atgcagtttt acatagcaat aacagtaaat ttgatgagtt aaagaattac
    13561 ataaataaaa gtgtatatga agagaactta aggtatgaac aagagattat tgatgaattt
    13621 agcttgatta ataaaacgaa agttcaattc aatgctatgg attaaatagg aggagaatca
    13681 tggtaaatta tacacaagaa atatatagaa agatgctaga tagtgcttcg tttcctggga
    13741 aattaaataa tatagataat ataaatatag aaaataatac aaaactattg aatggagcaa
    13801 ttggtatagc aatcacttta ttagaccaag aagttacctt ctatatagaa aattatactc
    13861 aaaaagatat aaaaaatata agagcgctta cagtgtcaaa tcaaatagat tatagaaaat
    13921 cagattatat atttttagat attaattcag aagtggatat attacagatt aaaataggtt
    13981 cttttgaata tcctgatgaa agtgcaacta tagtacatca agtaaatgat ttatcaacgt
    14041 actctcaaac taagtatata aagcttgatt tgagtggtcc tggtataaaa tctaaaaata
    14101 gtatatatat aaatggaatt agagaagaat ttatagaaag attatctacc ataaataaag
    14161 attatccaat aggaatagat ttgattttag tagataaaaa aggagaaatt gcatttatac
    14221 caaggtcatc aaaattaagt tgggaggtat tgtagaaatg gcttatgtag cagttaaagg
    14281 tggagaagaa gccatattac aggctaaaaa tatacttgag ttttataggg taaaaggaga
    14341 tagcttgcct atagatgtaa agcagattga ggaacaaatg tcttatgctg tagacaaagt
    14401 aatgtctgaa ggaagtttat acaacaagga actatcagca ttggctataa aacaagctca
    14461 aggtgatata ttagaagcct ctttctatgt aagagcttta aggtcaacaa tgcctagaat
    14521 tacatattca gaacccataa atacagaaga aatgagaata aaaagaagaa tatcagctac
    14581 ttttaaagat ataccaggag gtcagtatct tggggcaact agagactatg aaaatagaat
    14641 attaaatgta gaattattag aagaagatga aaaaaagaat agagatttta gagatagttt
    14701 tataaatgaa ttagaaacag tagatgaaat ccctgaattt acaaaaatat ctaatatatt
    14761 tagaaaagaa aatttattac aagaagaagt ttgtgaaaac aaattggaag aagttattga
    14821 tgatattaca agaaatagtt taagatttcc agtttctagg tcagcaaagt tacaggcatt
    14881 gactagaggt gaaagtggag tagtgtcagg ttttgcttat gctattgtaa gaggttttgg
    14941 tgatgaacat ccatatataa gtgaacttag aactggtaat gtatttataa aagtaaaaca
    15001 tcccatctat gaagatgaag aaataataat aggtgatatg ctggttactg aatgtgaaat
    15061 agtttcaaaa gcatcacaaa ataagactga aaattctaaa actaattcgg aagataaaaa
    15121 taagatgtct ttaggatatg gtctttgtat ggggaataat gagaataagg ctatatctat
    15181 ggccatacta gacaaatctt tggagccaaa tagtgaaaaa ctagcacaag atgaagaatt
    15241 tgtattgctt catatagatg gaatagattc attaggattt atatctcact ttaagcttcc
    15301 tcactatgta gattttaaag cggatgtaga aagaataaaa tagtttgtgg aggtaagttt
    15361 aggtgatata taactatggt tttttagacg aaaacacaaa aaaagaaata agaagaaaga
    15421 tattaaaagc aatatcaata cctggttatc aagttccctt tggttcaaga gagttgccta
    15481 tagctaaggg atggggtact ggaggtttac aattaacact atcattaatt ggtaaaaatg
    15541 atgtagtaaa agtaattgac caaggaagtg atgattctac aaatgcttgt aatataagga
    15601 gctttattgc aagtgtaagt aatgtagaaa ccacaaaaga tacattagaa gctacattga
    15661 ttcaaacgag acatagaata ccagaagaaa aacttaaatc taatcaaata ctaatatttc
    15721 aagtaccaat accagaaacc cttagaatag tagaacctag tgaagtagaa acaagaagaa
    15781 tgcattcaga agaagattat agtaggatgt gggtatatct ttatgaagat atagttagat
    15841 ttaatgatat atctatagca gttgaatatc catgtaaagt aaatgataga tatttaatga
    15901 atccatcacc tatacctaga tttgatataa aaaaattaaa tatgtcagac aatttattct
    15961 tgtttggtgc aggtagagaa aaaagaattt atgctattcc tccatataca aaggtagaac
    16021 ctttagagtt tgaggattat aagtttgaag aggaaaagtt tgaaggaaaa tactgtagtt
    16081 tatgtaacag tacaaataca tttttagatg aagtatatga cagtgacact aatgaaaagt
    16141 actatagttg ctcagataca agttattgtg aaaaagttag attaaaaaat aacagtatag
    16201 atgtgacaat aggaggtact tggaatgagt aaaataaatg tctctaaaag attagaaaat
    16261 atagacaagc aagtattaat aggaagaaat atcaatctta tttatggaga tgggtgtgaa
    16321 aagtgttttg aaagcacagg atatgaaagc aataatatat gtaagtattg tggttctata
    16381 gttgcatgta ataatgtaaa tttagaactt tatgaaggag aagtccttgg aattgtaggt
    16441 gaatctggtt caggaaaaag cactttatta aaaatactgt ttttccaaga acatgcaaat
    16501 agtggagaag tatatatatc ccattacaat aaagacacaa atatactaga gctttcagac
    16561 caaaaaaaga gatacataaa gaatcatttt atgggtattg tgtatcaaaa tccccacctt
    16621 ggtttaaatt tagactttag tagtggtggt aatatagcag aaaaattatt gatggcaaat
    16681 ttatataatg ttgaaaaaat aagagataga ggcaaagaac ttttaataaa aactaatata
    16741 cctgtagaaa gaatagatca taaaccaaaa tactttagtg ggggaatgca acaacgagtt
    16801 caaatagcaa aagctctttc aaataaccct ccaatattgc tattagatga ggttacaaca
    16861 ggtttagatg tttctgtgca agcggaagta ttggatttaa taagagaaat ccaaagagaa
    16921 cttaagattt ctatgatagt tgtatcccat gactttgatg ttataaagat gcttgctgat
    16981 aggacaattg taatgaaaaa tggaagtgtg attgagtctg gtcttacaga ccaaataatg
    17041 gaggacccac aacacccata tactcagcaa cttataaatt cattgctata aatatcgttt
    17101 gtaaataagc aaaatggggg tttttaataa aatgaaagaa actatacttg aagtaaataa
    17161 ttttagtaaa gactttgagt tacatgcatt gaacaagact ataacggcat gtagtaatat
    17221 aagttttgaa gtttcaaaag gtgagttttt aggaataata ggtaaatctg gagcaggtaa
    17281 gtctaccata ttaaaaagta tctataaaac atatatacca acaactggag aaataatttt
    17341 taattcagaa atatatggta atgtagacct ggctaaaatt ggagaccgag aaatgataaa
    17401 tcttagaaag aaagaaatag gatatgtatc acagttttta aagacacttc cacgaataac
    17461 tgcaattgaa ctggtagtac attctttaat tgagagtggg tttgaaaaag attgttcgta
    17521 tgatatggca aaagatatac ttaaccaatt tgaaataaag gaaaatttat gggatgcgta
    17581 tccaaataat ttttctggtg gagaaaaatt aagattaaat ttagctcaag ctatggttaa
    17641 aaagcctaga cttttgttac ttgatgaacc aactgcatca cttgataatc aatctaaaat
    17701 atatgtaaaa gaaaaaatgt tggaacttaa aagtcaagga actacaatga taggaatttt
    17761 tcatgatata gagtttatgg aaactgttat agataagacc tttactatgg caaaagggac
    17821 tataagtgaa agtggggtag cctagatgat aaaactaatt cataatgcaa atattgtatt
    17881 agaagatatg attttagaaa atggtcattt aatcatagag ggagatacaa taaaaaaagt
    17941 atcaaatgat aggattgatt acgcatttga ctttaatgag atagatgaaa taatagatgc
    18001 aaaaaactta tatgtaatac ctggtattat agatattcac agtgatgcca ttgaaaagga
    18061 aatagagcca agaccaagta cattattacc atttaatatg gcattttatg aactagataa
    18121 aaaactacct gcaaatggaa taactacagt ttatcattct atatctttag gggatggagt
    18181 aggagttaga agtattgata actctttaaa gatgattaaa aatattgatt catataaaaa
    18241 tatagatagt aagagtataa atcataaagt tcatttaaga tatgaagtgt tgtattatga
    18301 aggtttagaa aaggttttag aactattaga tgaaaataaa atagactact tatcaataat
    18361 ggaccattct ccaggacaag gtcagtatac aaatccaaca ttttataaag agtatgctac
    18421 taaagtgtgg ggagttacag aaaattatgt agatacatgg ttagatgatt tagtaaatct
    18481 gcatgataat ttagattgga ataaaatagc taatattata ggtatagcca aaactaaaaa
    18541 tataaatgtt gcatcacatg atgatgatac attagaaaaa atggatttca taaaaaatat
    18601 aggcataaat gtatctgaat ttcctataac tcttgaagtt gcaaaacatt caaaaaaatt
    18661 gggtgttcta acttgtttag gtgcaccgaa tattgtaaga ggtaaatctc ataataacaa
    18721 tttaaaagca atggatgcta tattggaaga ttgttgtgac attgtgtgtt cggattatct
    18781 tccatcagct atgataaagt ctatgtgtat agttgcagaa agaataaata atttaaataa
    18841 agcagtcagt ttatttacct caaatcctgc taaagcagtt ggtatatatg atgaaagagg
    18901 tagtataaaa gaaaataaaa aagcagattt agttttagta gatattgata gtgaatatcc
    18961 aaaggtttta aatactattg taaatggaaa aacagtttat aaaagggagg tataaaaatt
    19021 gctaacagag ttacattgtc atacaagtat atcagataat aattttacta ctgaagaaat
    19081 aataaaaaaa gcagcagaaa agaatataaa tatcttagca ataacaaatc atgacacatt
    19141 atctggattg gatgaagctc ttgtgcttgg agagcattat ggaataacaa taataccagg
    19201 tatagaaatt tcagcctatg attataaaaa tagaaaaaga gttcatattc ttggatataa
    19261 catagattta aacagtcaag aaataaaaaa tttgtgcaat ccaatggtat gtgacagaca
    19321 taaagcatct gttaagatgg taaataaaat tatagattta ggatacaaaa tatctttgga
    19381 agatgtaaag aaatattcat caaagactgg aatatttaaa caacatataa tgaaagctct
    19441 tatagataaa ggatatacag ataatatata ttcaagtcta tataaagaat tgtttcatag
    19501 aggaaacggc aaggtatatg tatctttaaa atatgtggat tacagagatg caataaaagc
    19561 tataaaaaat tctggtggaa tttgtgtgtt agctcacccg ggtcaaatgg ataattttag
    19621 cgctattgaa gagatggtaa aagtaggatt agatggaata gaagtatatc atccatctca
    19681 caacaaagaa ctagaaaaag agtcgctttc ttatgctaag aagtataatc tagtggtcac
    19741 aggaggctca gattaccacg gattttattc tcaacataat tatgaacttg gaagcaagag
    19801 cttaaataaa gaagatttat ttaagtttag gttagcttta ggaggggtat tataaatgtc
    19861 ttttttacaa gtaaaaaatg taggtaaaag ttatggacaa gttaaggtgt taaaagacat
    19921 atctattgat attgaaaaag gagagtttat ttgtttacta ggtccaagtg gttgtggaaa
    19981 aagtacactg cttagaataa ttgcagggct tgaggataaa catggtggaa aaataataat
    20041 aaatgataag gatatgacta attcaccacc tgaaagtaga aattttggta tagtttttca
    20101 atcatatgca ttatttccaa atatgaatgt ttacaaaaac atagcctttg gactagaaaa
    20161 taaaaacata agtaaatcta atattgataa aaaagttaaa gaagttctag acgttgtaga
    20221 attaagtgga tatgagaaaa aatatccatc acaattatca ggtggacaac aacaaagggt
    20281 tgcattagct cgtgcgattg ctttagagcc agacttttta cttcttgatg aacctttatc
    20341 tgcattagat gcaaaagtaa gacttaaact tagggagcag attagaagtt tacatagaaa
    20401 actaggcatt acaactataa tggtaacgca tgaccaagaa gaagctcttt gtttagctga
    20461 taagatggtt gttatgaata gaggagaaat aatacaagtt ggaacgccaa aggaggtata
    20521 taaaaatcca gaaacacctt ttgtagctga ttttatagga acaataaatt ttatagatga
    20581 tggtattaat aagatagcta ttagaccaga agatataaaa gtagagtcta atagagattc
    20641 taaagataag gatataaaag tgggagaaat tttagatatc gaatttagag gatttaacta
    20701 tagaattaca gttgagtatc gttctaagca aatgaaatta gatgtagttt ccaaagttgc
    20761 agaacaaatg aaactgtgca taggttctaa aataaacttt aagataccta aagaaggaat
    20821 tgtacaatat aaatcagagg gatgtgctta atagctaggg gtgaataaat gataacatca
    20881 aaaaataaaa gaagaataag tgatagcaca atacaaaaat atttcttgat tttttcggta
    20941 attgtactaa tagtatttat attatttcca ttagtatcat tattaaaaaa tgcatttatg
    21001 gatacaagtg gaaattttat aggaatttca aactttagta aatatgttga aaatccaagt
    21061 cttatagcat catttaaaaa ttcaattttt gtatctacag tctcatcttt aatatcacta
    21121 tgtttagcat ttatatattc atacgcaata acaagaacaa atataaagtt taaagatttt
    21181 tttaaaacta tgggaatgtt tccacttttt gcaccaacta tgttatatgg tatttcactt
    21241 atataccttt ttggaaataa gggaatcttt acaaccatgg gtctggaagt accactatat
    21301 ggtccattag gaataattat ttctcagatt ctatttacat ttccacaagc ttttttaata
    21361 ttatctgtag cactttcgat ggctgattat agactttatg aagcagcaga tagcttgggt
    21421 gctagtgaat ttaagaaatt ttgcaaaata acaattcctg gtataaagta ctcattactt
    21481 agttcattct ttgtatcctt tatacttgca tttacagatt ttggagcagc taaggtagtc
    21541 ggaggaaact acaatgtact ggctactgac atatataaac aagtagttgg acaatttaac
    21601 ataccaatgg gagctactgt ttctatggta atgttaattc ctgtactaat agcatttaca
    21661 ttagataaga tggcaagtaa gaaacaaggt atgaccataa ctgcaaagtc tatgccatat
    21721 aagattaagg aaaataagtt tagagataga gtatttttaa tagtatgtag ttttataaca
    21781 ttgctaattt ttattttaat attcgtttca gttataggtt cagttataaa gttatggcca
    21841 tataatttaa gctttacaat ggaacactat acatttaaag gtattttggg aagtggaata
    21901 aaaacatact taagcagttt gaaaatatca actttaacag caatatttgg aactatattt
    21961 gtatttttta gtgcttatat aatagaaaag gtggataagt ttcctaaatt aagacaaaca
    22021 gcttaccttt tatcaatgat accaatggcc ttacctggct tagttttagg tatatcatat
    22081 ataacttttt ttaactcagc aagtaatcca ttaaattttt tatatggaag tacagcaata
    22141 ttagttatag taaatgtagt tcatttttat tcagtttgtt ttataacctc aaattcatct
    22201 ttaaaaatgt tggataaaga gtatgaatta gttgcaaaat
//
LOCUS       DAEPOZ010000036        22066 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.036, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000036 DAEPOZ010000000
VERSION     DAEPOZ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22066
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.036"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(275..1372)
                     /gene="ychF"
                     /locus_tag="KR440_001123"
     CDS             complement(275..1372)
                     /gene="ychF"
                     /locus_tag="KR440_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBH0698181.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
     gene            1688..2953
                     /locus_tag="KR440_001124"
     CDS             1688..2953
                     /locus_tag="KR440_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBH0698182.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            2968..4254
                     /locus_tag="KR440_001125"
     CDS             2968..4254
                     /locus_tag="KR440_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBH0698183.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDKLSKSIERFYPEEPESVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDVIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            4651..5397
                     /locus_tag="KR440_001126"
     CDS             4651..5397
                     /locus_tag="KR440_001126"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBH0698184.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKDDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKHNISEE"
     gene            5427..6362
                     /gene="rsmH"
                     /locus_tag="KR440_001127"
     CDS             5427..6362
                     /gene="rsmH"
                     /locus_tag="KR440_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBH0698185.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGKLVDIIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNKGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            6848..7195
                     /locus_tag="KR440_001128"
     CDS             6848..7195
                     /locus_tag="KR440_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698186.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIAIFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            7207..9186
                     /locus_tag="KR440_001129"
     CDS             7207..9186
                     /locus_tag="KR440_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HBH0698187.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            9353..10726
                     /locus_tag="KR440_001130"
     CDS             9353..10726
                     /locus_tag="KR440_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBH0698188.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREASAQNAFVA
                     IVGENLDGHTFMKPAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDIYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            10767..11735
                     /locus_tag="KR440_001131"
     CDS             10767..11735
                     /locus_tag="KR440_001131"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBH0698189.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            11754..13109
                     /locus_tag="KR440_001132"
     CDS             11754..13109
                     /locus_tag="KR440_001132"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBH0698190.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEVVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            13161..14291
                     /gene="spoVE"
                     /locus_tag="KR440_001133"
     CDS             13161..14291
                     /gene="spoVE"
                     /locus_tag="KR440_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HBH0698191.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            14309..15535
                     /gene="murG"
                     /locus_tag="KR440_001134"
     CDS             14309..15535
                     /gene="murG"
                     /locus_tag="KR440_001134"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBH0698192.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            15805..16545
                     /locus_tag="KR440_001135"
     CDS             15805..16545
                     /locus_tag="KR440_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HBH0698193.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNINLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSVDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
     gene            16568..17266
                     /locus_tag="KR440_001136"
     CDS             16568..17266
                     /locus_tag="KR440_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBH0698194.1"
                     /translation="MKKRFINKSIIIGSVVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKTEKENLQSEVNSVKKDIETYRGKNNKGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVFNTYMY
                     LKEDKLCMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            17271..17945
                     /locus_tag="KR440_001137"
     CDS             17271..17945
                     /locus_tag="KR440_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBH0698195.1"
                     /translation="MKKKIVILGAFILGIFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKNNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VNLFAK"
     gene            18044..18397
                     /locus_tag="KR440_001138"
     CDS             18044..18397
                     /locus_tag="KR440_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HBH0698196.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            18587..19744
                     /gene="ftsZ"
                     /locus_tag="KR440_001139"
     CDS             18587..19744
                     /gene="ftsZ"
                     /locus_tag="KR440_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBH0698197.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            20028..21302
                     /locus_tag="KR440_001140"
     CDS             20028..21302
                     /locus_tag="KR440_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBH0698198.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            21593..>22066
                     /locus_tag="KR440_001141"
     CDS             21593..>22066
                     /locus_tag="KR440_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HBH0698199.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGSAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTI"
BASE COUNT         8819 a         2337 c         4008 g         6902 t
ORIGIN      
        1 aaaatacttt agatagtaat aatagtaatt tctataggtg tgttatacat attttaaaaa
       61 ataaaaagat aatttataaa tttaaatgtt atatatgtaa gtaattttat tatatcaaaa
      121 aaagagtttt tcataataat ttatacaaat catttgaaat actcttcttt tatttttaaa
      181 ttctatataa ttattataag ataatattgt attattataa tcttagtttt ttagttatta
      241 tatataatag tcaaagtgta tatgattaat cattttaaac attaaatcta aataatatta
      301 catctccatc tttaactatg tattctttac cttctaattt aaccaaccct ttttctttag
      361 cagcagccat agtaccatgc tcaaccaaat cattaaaggc tattgtttct gctctgataa
      421 atcctctttc tatatcagaa tgtatttttc ctcctgcctg aggagcttta cttccaactt
      481 ttatagtcca tgctctaact tcttttggac cagcagttaa gaatgaaatt aaaccaagta
      541 aagcataaga agattttata agcttatcaa gccctgattg ttcaagacct aatgtttcta
      601 aaaactcttt cttttcttca gcagaatcaa gttcagaaat ttctgcttct atttgtgcac
      661 atactacaac aacttcggca tcttctgttt ctgcaaatgc tttaacttgt tgaacatatt
      721 tattattttc tccattatca gctaaatcat cttctgatac atttgaagca tatattacag
      781 gcttagatgt aagtaaatcc aatgaattta cgaatgtttg ttcatcttct gtaaattcca
      841 tagttcttac acacttactt tcttctaaag tagacattat tgattttaat aaatcaagtt
      901 cagaacctaa agttttatca gccttagcag ctttttgagt tttattaatt cttctttcaa
      961 gaatctctat atctgagaaa attaattcta agtttatagt ttctatatcg cgaagtgggt
     1021 ctacagagcc atctacatgt acaacatttt catcctcaaa acatctaaca acatgaacta
     1081 ttgcatcaac ttctcttata tgtgataaga atttatttcc taaaccttct cctttagaag
     1141 cacctctaac taaacctgct atatcacaaa attctatagc agtaggaact attttttcag
     1201 aattatataa ttcttgtagt ttgtttaatc tttcatctgg aacagataca actcccacat
     1261 ttgggtctat tgtacaaaaa gggtaatttg cagattctgc acctgcttga gttattgcgt
     1321 taaatagtgt gcttttacct acatttggca aaccaactat acctaatttc atattaatcg
     1381 tcctctctct tattcttttt tatattcttt atataacttt aaacaatcaa tctataggat
     1441 tgcaaaattg ttttaattat agacatacat attgattata catcatatat atcaagcttg
     1501 taaactagac tttaaagcat attgatttaa ataatatttg catttaattt aaataaaaat
     1561 attgctttta gaaaataata aaaaggatat aattaaatag aaggttttta tatgtgagta
     1621 tatataatta aaaaataaat aaatttcaat tttaaaattg atgtttaaat aaaaatagga
     1681 ggtacatatg gttaatacta aaaaaataat aaatttaggt aataatatta atcttacttt
     1741 gataaaaaca gaaaagttta aatcgaatct tataagttta tatgttcaga gactattgga
     1801 tgaaaaagaa actacaaaaa atgctctaat accatcaata atagcaagtg gaagcgctaa
     1861 atatcctagt gctagagcaa tatccaataa attagatgat ttatatggtt catctatggg
     1921 agctgatgct gtaaaacgtg gagaacgtca agtattgagt tttaaagtaa ttaacataag
     1981 tgaaaagtat ttagatgaga gtatatttga agaagtagta gaatttttta atgaagtaat
     2041 aaataatacg cttgttgtag atggtgggtt taaagaagaa tatttaaata ttgaaaaaga
     2101 gaatttaaga gaaaagatac aatcaataat aaatgataaa aaggaatatg cgcaagataa
     2161 atgtattgag gctatgtgta aaggagagag atatagtgta agtgaatttg gttatgaaga
     2221 tgaaatagat agtataactt caagagagtt atatgaacat tataaaaaca tacttaaaac
     2281 atcacctata gatatagttg tagaaggtaa ttttgatgaa gataaagttg tagatataat
     2341 aagtaaaaat ctaaaatttg aaagagaaga aattataaat ataccaagag cagattttat
     2401 aaaaaatgtg gatgaggtaa aagttataga tgaacaaatg gaaataactc aaggtaagtt
     2461 agttatgggt tatagagcaa atgtggatta tgcagacata gataaatatt atgctttagt
     2521 agttgggagc aatgtcttag gtggtggtcc acactctaaa ttatttgtta atgtaagaga
     2581 aaaagaaagt ttatgttact atatattctc ttcaatagag aagtataaaa gtatcatgtt
     2641 tatatcatct ggtatagaaa ctaaagatta tgataaagct gtagaattga taaaaaaaca
     2701 agtagaaagt gtaaaagcag gtgatatatc tgatgaagaa ttagaaaaca gtaaacttgc
     2761 tttagttaat tctatgaaat ctataactga taatataggt ggtatgtctg attttgtttt
     2821 ctctcagtct atggcaaaga caaattctga agtacaagac ataatatcta gtatagaaaa
     2881 ggttacaaaa aaagatatag ttgaagctat aaaaaatata gagcttgata ctgtatactt
     2941 cttgagaaat taatggagga gatgtctatg gaaaaaatag ttaatgatat attgaaagaa
     3001 gaagtttatt atgagaaatt gcaaaatgga ttagatgtat attttatgcc taaaaggggt
     3061 tttatgaaaa agtatgctat tttggctact aattatggtt caaatgactt agaatttgta
     3121 ccaattggag aagataagaa aattagagtg aatgaaggta ttgcacattt tctagagcat
     3181 aagatgtttg agcaacctga tggaggagat gcatttgata aattttcaaa attaggagta
     3241 aatgcaaatg catttactaa ttttacaatg acagcctact tattttctgc tactgaaaat
     3301 ttttatgaga gtttagaaca tctaattgac tatgttcaaa caccttattt tacagatgaa
     3361 aatgttgaaa aggaaaaagg tataatagca caagagataa aaatgtacaa tgatgaccct
     3421 gattggaatg tatactttaa ttgcttgaag gcaatgtatg taaattatcc tgctagaatt
     3481 gatatagcag gtactgttga tagtatatat aagataacta aggaagagct atataaatgt
     3541 tataatactt tctataatcc aggaaatatg gctttatttg ttgttggtga cttagatgta
     3601 gaaaaagtta tagatgtaac taaaaagtca aacaattaca aagtagacaa actctctaag
     3661 agtatagaaa gattttatcc agaagaacca gaaagtgtaa aagaaaaaga ggttatagaa
     3721 aaattcccta tatctatgcc tatgttcaac ataggtttta aagatagtaa tgttggttta
     3781 aaagggaaag aacttttacg taaagagatt gtaactgata tacttgtagg gatgttattt
     3841 aaaaaaggaa gtaagctcta tgaagatttg tatatgcaag gtttaataaa tgaaaatttt
     3901 ggagctggat tttcgtctca agtggattat gcttttagca taatagctgg agattctaaa
     3961 gagcctaaaa aagtaaaaga aataattctt gattatatag aaaaatcaaa aaaagaagga
     4021 ttatcaaaag aagagtttga gagaactaaa aagaaaaaaa tagggtcatt tattaagtgc
     4081 tttgattcta taaactttat aggtaatagt tttatatctt atgtttttaa ggatataaat
     4141 ctattggatt atttagatgt tattaaagat attacttttg aggaagttga agaaagatta
     4201 aaagagcatt ttaaagaaga gtattgtgtt atttctattg tagaacctaa atagaaactt
     4261 aatcataata agaataacta attaagtatt atttatgttt ttaaatgttt attttggtaa
     4321 aacaaattta taaaatatat ttatataaat tgaggtaaaa atttaagagc tatagataag
     4381 gaatagaaga ggttgatgta tatatgcatc aatctttttg attaaaaaat atctatgtac
     4441 acttgtaact ttagtcatga gagattcagt ttaaaaaatt ttatagtaat tatattagta
     4501 taatatagtg tagggatttg tagaataaat tttaatattt tttgtagaat taacattaat
     4561 gtgttatcat aatttagtat aattacaatt aattgtaatt atagcatatt gctttggaaa
     4621 tattttcaat taatacaaag aggtgacgtt atggatagag tagcatttac actgtttggc
     4681 atagacatca tgtggtatgg aatacttatg gcatgtggaa tgattttagg aactttaata
     4741 gcaataaaag aagcaaaaag agtgggaatt aaagatgatg atgtattaaa tatagctata
     4801 atagcaattc cagttggact aatctgtgca agaatctatt atgttgtatt taattggagt
     4861 tactatgctc aaaatatgtc tcaaattttt aattttagag gtggtggact tgctatacat
     4921 ggagggttaa ttggtggaat attagctggt tatatataca ctaagattaa aaatattaac
     4981 ttcttaaaaa tggctgatac agttatactt ggaatgcctt tagcacaagc tattggaaga
     5041 tggggaaatt ttattaatgg agaagcacat ggaggtgcta ccaatttacc ttggggaata
     5101 atggttgatg gagtaaaggt acatccaact tttttatatg agtccatatg ggattttgga
     5161 atatttatag ttttattact atttagaaaa aataaaaaat atgaaggtca agttatagtt
     5221 acttatataa ctttatattc aataggaaga ttttttatag agggattaag aacagatagt
     5281 ttaatgttag gaccacttag aatggcacaa gttataagtc taattggagt tattggtggc
     5341 ataattgcac atgtttattt atcaaagaaa aataaacata atattagtga agaatagttt
     5401 aaaaagtgaa tttaagggag gtaactatgg aatttcatca tgtatcagtt cttttaaatg
     5461 agtgtataga gaatttaaat ataaagccag atggcgtgta tgttgactgt actatgggag
     5521 gagcaggaca ttctaaagaa atagtaaaaa aactgtcaga taagggatta tttatagggt
     5581 ttgaccaaga taaaaatgct ataagcacag caaaagagag attgagtgaa tatgaaagta
     5641 gagtgaagtt tgttcacagc aattttgaaa atataaaaga ggaattagaa aaaataggag
     5701 tttacaaaat tgatggtgta ttagctgatt taggagtttc atctcatcaa cttgatgaag
     5761 ctgatagagg attttcttat atgcaagatg caccacttga tatgagaatg gatgttaggt
     5821 gtgagttttc tgcatatgat gttgtaaata cttatacaga agatgaatta actaagataa
     5881 taaaagatta tggagaagat aactgggcta agcgtatagc taaatttata gttgaagaaa
     5941 gagcaaacaa accaatagaa actacaggaa aattagtaga tataattaaa aaagctatcc
     6001 ctaagaaagc tagaatagat ggacctcacc cagctaaaag aacttttcag gcaatacgaa
     6061 tagaagtaaa taatgaactt ggagtaatta caaagatgat aaatgatgct tcttctataa
     6121 tgaataaagg tggaagaata tgtataataa cattccattc attagaagat agaatagtga
     6181 aaaatgcatt taagcattta gcatcagatt gtatatgtcc acaacattta cctatatgcc
     6241 aatgtgataa agaatctgag gtgaaaataa taactagaaa acctatatta cctagtgagg
     6301 aagaaataga agtaaatcca cgttcaagaa gtgctaagct aagagtagca gaaaaaatat
     6361 aagtagttat aaggctaaag aataatctta tatataaaat aagattacta taattaatta
     6421 aaaagtatta tatattttgt tgaactggta aagcaattaa aaaacaatta acactataag
     6481 aaatgttctt tgttattgag atatataatg aaaatttaga tataaaaata tagatataga
     6541 tggtattaag taatataatg gagaacttag gaatcagaga gtagatagtt agttacatat
     6601 tatatttatg gaagaaatta tctaaaaata ctagaagtga taaaaataaa tagaaattaa
     6661 tatctaacct atagattcat ataaaagtat aagtgaattt ataggttttt gttgtgtata
     6721 gaaaaaaata caattagatt gtgtaatagt gtattaattt agcgatgaaa ttttagagga
     6781 agaaatatga ttaataacaa atatagtata gaggaaaatt taaactatat actatcgggg
     6841 tgagaatttg aaaaaaaatt ataaaataca taatctaaat gaatataaaa ataaaaaaaa
     6901 gaataattat aaaaagagaa ataagaaaaa aataggagct aaatgcattt tactgattgc
     6961 aattttttca gtaataatat tgaatctatg tggttattca aaaatctcgg aaatgaagta
     7021 cgatatctat tatttacaaa aagatttaag aaaaaaagag atagtattag aggaactaaa
     7081 ggcaaaagaa actgaaaaaa catcaataaa agaaattgag ctaaaggcta aggaattgct
     7141 caatatggac tatccaaaag atggacaaat aaagtatata gatgtggata actaggggga
     7201 atagatttga gaaaagtaaa gaggataagt aagaaaaggc tggtactcgt tcttattcta
     7261 gcatgtgcat tgtttttttg ccttgttatt aggactgggt acctacaact tatgaaggga
     7321 aattggttga gtacaaaagc actagaacaa caaacaagag atataccaat agaacccaaa
     7381 agaggaacta tatatgatag aaatatgaaa gaattagcag taagtgtaac taagtatact
     7441 gtttggtgta aacctgtgga agtagaagat aaaaaagaag cagctgaaaa ggtagctgag
     7501 attttggatg aagattataa agatatttac gctttaataa gcaaaaaaaa tatggctctt
     7561 gtaaaagtaa aaagatggat agatgatgat aaggcaagtc aaataagaga tgctaaatta
     7621 agtggaatat gggtagcaga agataatcaa agatattacc catatgggaa ttttgcgcca
     7681 tatgtgttag gacatacttc ttcagatgca acaggtatat ctggtgtaga aatgcaatat
     7741 gataaaaagt taaaaggaaa gcctggaaaa cttatagtaa gtactgatgc ctcaggaaga
     7801 gaaataccac aaggaatgga aaaatattat gagcctgtac aaggtaatgg tcttgtgttg
     7861 tctatagatg aagtaattca acactatact gaaaaagcag tacagaaagc ttatgagtta
     7921 aataatgcaa aaaaggttac tgcaatagct atgaacccta aaacaggtga tatacttgct
     7981 ttagcatcaa aaccagatta tgaccctaat gactcaagaa cgccaatata tccatattat
     8041 caagaagaac ttgaaaaata taatgataaa gataaaataa aaggctatta tcaaatgtgg
     8101 agaaatccag cagtaagtga tacatatgaa ccaggttcta catttaagct tattacatct
     8161 tccagtgctc ttgaagaggg agtaattaaa gatggagaaa aattcacatg tacaggtagt
     8221 gtaactgttg gaggaagaaa gattaaatgt tggagacatt acagacctca tggaacacaa
     8281 gaatttaaac aagcagtaca aaactcttgc aacccagtat ttgttgaatt aggaagcaga
     8341 ttaggtgttg gaaaaatgta tgattatata gaatcatttg gccttatgga taagacaggt
     8401 atagatttac caggagaagc taagggaata ctgtataacg aaaaaaatgt aggaccagtt
     8461 gaattggcta caatatcatt tggtcaatct atatcagtaa ctccaataca acttataaca
     8521 gctatatcat caatagctaa tggtggagat ttaatgcaac caagagtggt aaaatcttat
     8581 actgacaata aaggaaatat aactgaaaca gtaaaaccaa aaaaagtaag aagtgttata
     8641 tctaaagaaa catctaaaaa aatgttagaa attgcagaat cagttgtaac tgaaggtgga
     8701 ggaaagatag catatatacc agggtataga cttggtggta aaacaggtac agctcaaaaa
     8761 gttatagatg gtaagtatgc tccaggaaag tatatatgtt catttgttgg aatagcacct
     8821 tgtgatgacc cgcaaatagt agttcttgct attgttgacg aaccaacagg agtaagtgca
     8881 tttggtagta ctacagcagg accaatagtg aaagaaataa tgaatgactc tttaaaatat
     8941 ttgggagtta aaccagtata taaagaagaa gaaaaagctg agtatgaaaa gaagcaagtt
     9001 aaagtgccag atgtaagaaa tctaaaaata ggagatgctg taaaggcatt ggaggatgcg
     9061 aagttaaaac cagacttaga tgcagatata gaacttccag aagatacaaa ggtaaaggat
     9121 atattcccaa aacctggagt aaaagttaat gaagactcaa gtataaccct atattttgaa
     9181 aactaaaaag taacagataa ttaacatata cccataaaca tcttcaaaaa aacagaaata
     9241 tagtatataa ttataaagtt aagagttatc gtaggcaatg tatatataac ggtatacatt
     9301 gccttttgtt gctaagagaa tatagctaag gttttaaagg aggagtttaa taatggagtg
     9361 cttaacaata aaagagttag tacttgcaac taatgggaag ttaatatatg gagattataa
     9421 tgactgtgta agtgatatag ttatagatag cagggaggca agtgcccaaa atgcatttgt
     9481 agccatagtt ggagaaaatt tagatgggca tacatttatg aagcctgcat atgatagtgg
     9541 atgtaaaact tttataaaaa atgaaagtaa tggaataaaa ttagaaagtt cggatataaa
     9601 tttaatagag gtaaaagata catctttggc tctaggagat atttcaaaat actataaaga
     9661 aaaatttgat attccattta taggtgttac aggtagtgtg ggaaaaacta ctactagaga
     9721 tatgatttac tctgccatat ctgcaaagtt aaatatacta aaaaatgaaa aaaacttaaa
     9781 taatcatttt ggagttccat tgactttatt taatctaaat aaagaacatg agtgtgctgt
     9841 aatagagatg ggtatgtctg gattcaatga aataaaatat ttagtggata tagtaaatcc
     9901 taaaattgca gtcatatcga atataggatt atcacatgta gaaaaactgg gttcacaaga
     9961 gggtattttg aaagcaaaga tggagattac ttcaaacttt gatgaaacaa atactttaat
    10021 tgtaaatgga gatgataagt ttttatctac attaaaagaa aaagaacatg tatataagtt
    10081 aaaaacattt ggctttaata aaaacaatga tatttattgt gaaagttata ctatggaaga
    10141 ggatagttta acatttatat gtgtaataaa tgggaaaaaa gaggaaatat ttataccaac
    10201 aataggagaa cataatatat ataatgcaat gtctgctata ttagttggtt tgtgtctaaa
    10261 tatttcttta gattatataa aaaaaggttt aaaaaatttt aaagggacaa aaatgagact
    10321 agacataata aaaaatgaaa aattaacaat aattaatgat tcatataatg ctagtccaga
    10381 ttctatggat gctgctttaa agatacttgg aagatataaa ggtagaaggg tagcgatact
    10441 tggagatata ctagaaatgg gtgaaatttc agagtatggt catagattag ttggaaaatc
    10501 atctatgaat aatactgata ttataataac tataggtgaa aattcagtgt ttattggaga
    10561 agaagcaaaa caattaggat tcaattcagc caatatatat cactttgaga atagagaaga
    10621 tgtgtttaac aagttaaatg aattagtaaa aacaggagac actattttag taaaaggctc
    10681 aagaggcatg agattagaaa aaatagtcga atatctaaat aaataagctt aaactaatga
    10741 gttaattgac aagggagagg agttttatga tgttaggaat aacagagctt acgtacaccg
    10801 cattgattgc gtttttaata gtaattataa taggaccaat atttatacca atgcttagaa
    10861 aatttaaatt tggtcagaca gttagagatg atggacccca aacacatcta gctaaaaacg
    10921 gaacacctac tatgggtgga attataatga ttgttgcaat tctaataact ggacttacaa
    10981 gagtaaaagt tagtcatgat atggcagtag gtcttatatg tattgcaggt tttggtttta
    11041 ttggcttttt agatgatttt ataaaaataa agctaaaaag atcacttgga ctaaaagcat
    11101 atcaaaaaat aatccttcaa gttgcattat ctttttatgt tgctttttat cagtatacat
    11161 catcatcaag tgcttcacaa cttatgatac catttacaga ttttgtgata aatgttggaa
    11221 tattatatat accaataatg atgtttataa tcgtagcaat agtaaatgct gtaaacttga
    11281 ctgatggatt agatggatta gcatcaggag taacattaat agtttctgta ttctttatgc
    11341 tatttgcaag ttctattgct ggaaatacag aagtcgcagt tttagcagca gcaactgttg
    11401 gagcatgttt aggtttctta ggatttaatt catatccggc tagagtgttt atgggcgaca
    11461 caggttcaat ggcattagga ggtgcagttg tagcattttc agttctaact aactcagtgc
    11521 ttataatacc tataattgga ggaatttatt ttgcggaggc tctatcagtt ttgattcaag
    11581 ttggatattt taaggcaaca agaaagagat tctttaagat ggctccaata caccaccact
    11641 ttgaacagtg tggttggcca gaaacaagag ttgtatttat tttttggatt ataacagttg
    11701 ttttagcttg gataagtata atagcagtat tttagggcta gggggaatga aaaatggact
    11761 taaaaggaaa aaaagtttta ttagtaggac ttgcaaaaac tggtatatct acaattaaac
    11821 atttagataa attgggtgca agtataatcg taaatgatat aaaagatgaa aataaattga
    11881 gaaatatact tgatgaattg aaatctataa atgacataaa atacatatta ggacatcacc
    11941 cagaagatgt agatgatata gatatggttg tagtgtctcc aggtgtacca ctagacctac
    12001 cattcatatt gaaattaaaa aattcaggca agtatataat tggtgaggta gaattagctt
    12061 ttaaactatc gaataatcca atttttatag ggattacagg tacaaatgga aagacaacta
    12121 caactagttt ggtaggagaa attttttcaa gagcaaaacg agatacatat gtagttggta
    12181 atataggaaa tcctgtaata gatactatag aaacttcaag tgaagagtct gtgttagtaa
    12241 ctgaactaag cagttttcag cttgagagta tagatgaatt tagacctaaa gtaagcgcaa
    12301 tattaaatat tacagaggac cacttaaata gacaccatac tatggaaaaa tacatagaag
    12361 caaaggcaaa tatatttatg aaccaaactg ttgaggattt ctgtgtgtta aactatgatg
    12421 atgaggtagt aaaatcttta gctgataaat gcaatgccaa agtaatttac ttttcaagaa
    12481 ctaaaaaagt aaatggagga gtatatttag aaaataatga cataataata gatatagatg
    12541 ataaaatcaa atttctaaat aaagatgatg taagtttacc tggaggtcat aacttagaaa
    12601 attgtatggc agctatagca atagcatatg tctgcaaaat tgatttagaa gttataaagg
    12661 atgtacttat gacatttaaa ggagtggaac acagacaaga gtttgtaaga aatttagaca
    12721 atgtaatata tgtaaatgat tcaaagggaa ctaatccaga ttccactata aaagctatac
    12781 agtcttatga tagacctata atacttatag caggggggat ggataaggga agtaattttg
    12841 atgaacttct tgaaactgca aagtcatatg ttaaatcatt ggtcttatta ggagaaactg
    12901 catcaaatat agaaaattgt gctaaaaata aaggatttaa tgacatacat atagtaaaag
    12961 atatggaaga agcagttaag acttcttatg aaatttcaaa aagtggagat atagtgcttc
    13021 tttcaccagc ttgtgcaagt tgggatatgt atgaaagttt tgaagtaaga ggaaaagact
    13081 tcaaagataa tgtaaacaat ttaaaataat ctcttacctc taaacttact taaatggaag
    13141 aatggaggta gattagttgg atgcctaaag aaaatttgaa aaaacaaatt gacataagga
    13201 tgaagactga atttgatgga gtagtgtttt acactactat gcttttagta tttgtaggaa
    13261 tagttatggt gtttagtgca agttttatac aatcttcatt taaacacaat gatgcgtact
    13321 attttctaaa aaagaatgta atatatgctg tacttggatt tatagttatg attattacat
    13381 caagaataga ttatagtttt tggaagaaaa atgctacagc aattggagct atagcagtag
    13441 tattattatt gttagttttg acccctttag ggatagaagc aaacggagca aaaagatggt
    13501 tgggaatagg agctttaaca tttcaacctg cagaaatcgc aaagtttgct acaataatat
    13561 taactgcaaa attaattgaa aaaaattatg ataaaataaa atctttgaca aaaggtgttg
    13621 taccacttct cgtagttcca gggatatttt ttgcgttgat aatacttcag ccaaatttgt
    13681 caacagcagg tactgtaatt ctagttactt ttgtaatgat atttgttgct ggaatggata
    13741 tgaaaattgt atttgctatg atagggagtg gggctgcttt atttgctgca ttagtaattg
    13801 ctgaacccta tagacttagt cgtgttacat catttttaga tccttttcaa gaccctcttg
    13861 gtaaggggta tcaggtaata caaggtctat atgcactagg ttcaggtgga ttgtttggtc
    13921 taggcttagg aaagagtaaa caaaagtatt tttatatacc agagccacaa aatgacttta
    13981 tatttgctat aataggagaa gagttaggat tgattggttg tataatagtt attatgctat
    14041 ttgtagtttt agtctacaga tgtgtaagaa tagctttgaa gacttctaat gtatttgctt
    14101 gtatggttgt tataggtata ggtgcacaaa ttggaataca agcagcttta aatatagcag
    14161 ttgcgacatc atcaatgcca gctacaggtg tagcactacc atttataagt tatggaggaa
    14221 cttcactcac catctttatg ggagctgtag gaatagtgtt aaacatctca aagcatgtca
    14281 aaattaatta acaggagatg atgatttgat gaaagtttta ttatcaggcg gtggaactgg
    14341 agggcacgtg tatccagcta tagctattgc aaacaaaata agagatgagc atccagacgc
    14401 tgagataatt tttgttggga cggaaaaggg aatagagtca gaaatagttc ctaagtatgg
    14461 atttgaatta aagactgtaa cagttcaagg ttttaagcga aaaatagatt tcgataatgt
    14521 aaaaagagtt tttaagcttt ttaaaggttt ggagcaatca agaaagattg ttaagaaatt
    14581 taaaccagat atagttatag gaactggtgg atatgttagt ggacctgttt tatttaatgc
    14641 atctatgggg aaaataccag ccataataca tgagcaaaat tcttttcctg gtgtaactaa
    14701 taagatttta tctaaaacag ttacaaaggt acttacaagt tttgaagatt cgcataaaag
    14761 atttccagag gcagcagaag ataaattagt ttttacagga aatccagtta gaaaagaaat
    14821 tttattatca agaaaaaata ttgcaagaaa gaatctatca ataagtgacg aaaaaagaat
    14881 ggtactttgc tatggtggta gtggaggttc aagaaaaata aatgatgcaa tgagacttgt
    14941 aataaagaat atggtaaatg aagatatagc ttttatattt gctacaggca aaagctatta
    15001 tgatgaattt atgggaagta ttagcgatat aaatcttaaa ccataccaaa aagttgtgcc
    15061 ttacttagaa gatatggcaa atgcacttgc agcaagtgat ttggttatag gtagcgcagg
    15121 agcaatttca ttagctgaga taacggctct tggaaagcca tctataataa tacctaaagc
    15181 ttatactgct gaaaatcatc aagagtataa tgccaagagt atagaaaaac aaggggcagg
    15241 aattgctatt ttagaaaaaa atctaactcc agagagccta aatacagcag tatttaaact
    15301 tttaggagat agagaacttt tagttgatat ggcaaatgca agtaaaacta taggtaagcc
    15361 tgaagccata gaccttatat atgatgagat aatgaaagta tacaattcaa cacaaaagtc
    15421 aacttctaaa aaaacaaaaa aggaaaaagt tattaaggaa gtaaaagaaa taaaaaaaga
    15481 aacgactcca agtatagaag gtcaagctaa ggtaatagga ataaagaaaa gataagtaaa
    15541 tatttacccc taatttataa tatattagat tttctaaatt attataaatt aggggtttat
    15601 ttgtatgtat acagatttaa atatataaat agtatgtagt atactatgtt tattttgatg
    15661 aaatattgat ttatttataa aatttaatac cttatttgga tgttatgtaa taaaatgcaa
    15721 aaatttgact ttaaatatta taattttttt gtaggaaaaa caagctctaa aggtgtattt
    15781 attaaccaaa ggggttgatt atatttgaaa aaaagaagaa agttaaatac gaataatgtc
    15841 atgatagttt tagtttttat acttatgtta tctttttgta tatgtataat tgtaaaaagt
    15901 gatttatttg atgtaaagaa aatagatgta attggaaata agagggtgac caaatcaaat
    15961 ataatgaaag aactaaatat aaatctaaat gaaaatattt ttgcatacaa ttttaaagat
    16021 atgaaaaata aacttataaa aaatccatat atagaaaatg tggaaataaa aagaaaactt
    16081 cccaataaga taattataag tttaaaagaa aaagaaatat ttgcagtatt aaaagatgaa
    16141 gataattatt gttatataga taaaaaagga aatttattgg aagaactaag aggttctaat
    16201 gaaagtaaaa aagacttgat agtagatgta gattatagcg ttgatgataa taaatctata
    16261 aaatttaaaa attataaaac taaggagaat gtatttaaaa ctttaaatta tttaaaagaa
    16321 gaaggaattt atagaaaaat aaattatgta aacttaaaaa aagaaagcaa tatagaaatg
    16381 cttacaagaa gtaatataaa aatcttactt tctaatgatg ataacttaga ttataatata
    16441 tctagggtaa gtaaaattct gattgacctg caaaataaaa atactaatgg agggactata
    16501 aatcttaact atggaaagtt ggcagtgtat agtccagaag gatagacaat tgtaaggaag
    16561 tgatatattg aaaaaaagat ttataaataa aagtataatc ataggaagtg ttgtattggg
    16621 tgttcttgtt agtttacaac ttaaaattat taatatagaa aatagaggga tgacaacttt
    16681 taaaagagga gagcaactta cagaagaact taaatcttta aaaacagaaa aagagaatct
    16741 acaatcagaa gttaatagtg tgaagaaaga tatagagact tatagaggca aaaacaataa
    16801 aggagaacaa gacatactta agtcagaaat agaaaaatat gaaacattag ctggatatac
    16861 agatgttgaa ggaacaggca taaaaattga gataaattct acaaaagact taggaaactc
    16921 tgataaaaat gatagtatac tatataatta tgatttatta ttgtcaatga taaataaatt
    16981 aaattcagca caagcaagtg ccatatctat aaacgaccag agaatagttt ttaatacata
    17041 tatgtattta aaagaagaca agctttgtat gaatgacact gtcataaaag aaccaattat
    17101 aataaaagca attggtgatt ctgatacatt ggcttcggca cttaatataa agtatggtat
    17161 tgtctgggaa atagaaacat attataatgc caaagtaaaa gtgaccaaag aagacaatat
    17221 agaaataaat ggtcatagtg atataaacaa agcagagggg aaataatagt atgaaaaaga
    17281 aaatagttat attaggagca ttcatattag gcattttcat atcaacttat ataaaatcac
    17341 tagacccaaa tagagtatac ataacattag gtcaaactga aagtattgaa agtgaggtag
    17401 aaaatacaaa aaaagaaata aaaaatttaa aagaaactct taaaaacaac aaaaaaactt
    17461 tgaatgaata taaagaagca aaaaagaatg gaaatgaatc tatccaaaca cttatgcaaa
    17521 aagagcttga agaagttaaa gaattaagtg gttattcaac tgttacaggg ccaggcataa
    17581 ctattactat gagggatagt gagagagagt taaaagacgg acagaatcca aatgatttga
    17641 ttatacatga tatagatatt ttgagagttt taaatgattt aaaaaaggct ggagcaagag
    17701 caatttctat aaatggggaa agagtactag caacaagtaa gataaaatgt tcaggagcta
    17761 ctattacagt aaatgatact acatatggac aaccatttat aataaaagct ataggagata
    17821 tagatttatt gagtgcagct ataaactctc cagaatcata tgctaaatct cttaaggata
    17881 tatatggaat taatataaaa gttcaggaaa ataatgggaa taaagtgaat ttatttgcaa
    17941 aataaaacga aaaatacaaa aaaataaaga aaaagtaaaa aaaattaata aaaaggattg
    18001 taatgtagtt gtgtttcaaa gtaaaataaa gaggtgaaga tttatgttgt gggttttagg
    18061 aggattagca tttggtgtag tagtaggtta ctatattcca tttacatatt ctatggatta
    18121 ttccatatat atgtcagtag ctattcttgc aataatggat tctatttttg gagcgataaa
    18181 atccaatttt gaagataact atgataatgt aatttttatt actggtttca taggtaatgc
    18241 tatattggct atattattga cttatatagg agaaaagtta ggagtttctc tttactatgt
    18301 tgctgtacta gtttttggta tgagattgtt taataactta tctataatta gacgatatat
    18361 tattagtaat attatgaaca aaaaacgtaa aaattaaaga gcatttaata tatacgtaaa
    18421 ttattttata cttaaagagt aatatttgac aatataagta agaaatttaa aaaggaaaat
    18481 ttacgttttt gtggaatatg ttacttaaag gttaacaaaa taaaagaaga aaatgatata
    18541 attaatacat acgataactt tgaatcatga gagaaggggg tttatgatgc taaactttga
    18601 cgtagaatta gaagaatgtg cacaaattaa agtaataggt gtaggtggcg gtggaaacaa
    18661 tgctgtcaat agaatggtag aagctcaact aaaaggcgtt gaatttatat cagtaaatac
    18721 agataaacaa gctttatata catcaaaagc tgaatataaa gttcaaatag gagaaaaatt
    18781 aactagagga cttggagcag gagcaaatcc agaagtaggg aaaagagctg ctgaggagag
    18841 taaagatgaa atagtaaagt tacttcaagg agctgatatg gtctttgtaa cagccggaat
    18901 gggcggtggt actggtacag gagctgctcc agtggttgca ggcttggcaa aagaaatggg
    18961 gatacttaca gtaggtgtag ttaccaaacc ttttgcattt gaaggtaaga taagaatgaa
    19021 gaacgctgaa ggtggaatag cagaacttaa atcaaaagta gatactctta taacgatacc
    19081 aaatgataga cttttacaga tagtacaaaa aaatacatca atgttagatg cttttgcagt
    19141 tgcagatgat gtattaaaac aaggtataca atcaatttca gaccttatag cagttgaagg
    19201 gttaataaac ttggactttg cagatgttac tactataatg aaagacaagg gacttgctca
    19261 tatgggtata ggtagtgcta gtggtgaaac tagagcaatt gatgctgcaa gacaagctat
    19321 tcaatctcca cttctagaaa catcaataca aggagctaaa ggagtacttc ttaatgttac
    19381 aggtggtcct aacttaggat tatttgaagt taatgaggca tcaactttag ttatggaatc
    19441 ctgtgaccca gaagcaaatg ttatatttgg agcttctata aaagaagatt taggtgatga
    19501 aataatgatt acagtaatcg caacaggatt tgaaggatta caaaatggtg ccttagattt
    19561 agatactaaa cctaaatcaa gcatacgttc ttcattaaat acaacagtaa aacaagctgt
    19621 aaaagaaatt gaagaagaag taattgctga ggaaaaaata gagcctccta aaaaggcaag
    19681 tataattgaa gaagatgatg atgagagcat ggagatacct acattcctaa gaagaagaag
    19741 ataatatgta agcttataaa gaagtcaata gacttctttt tattttgttc aatttagata
    19801 gttagtttgt ggctagactt aaaaatattt ttaaatattg ataaaattta aaaagtaagt
    19861 ttttagtatg aattaaatct aataggtata gaaaaaaaat atagttatag gagttagtat
    19921 agttttttat gattagacaa gagtagtctt atttgttaat attaaatctg aattaaatgt
    19981 aaacatttga ggaaaaagac aaattaaagt ctgaaagggg attaagcatg attaaactaa
    20041 aaaaggttct ttctttagtt atggtaggga ttctatcagt atcaattctt gcaggatgtt
    20101 caaaaaaaga agattctaaa ggcaaagagg gtaaagaaaa attagttgta tggtctgcac
    20161 cattaacaga ccatgatgca gatgcatgga agccgatatt tgaaaaattt gaaaaagaaa
    20221 ataactgtga aattgaattt cagatagtac catgggataa ctacgcagaa aaatatgcaa
    20281 cagcaataag tgcaggtgaa ggtccagata taggatacat gtatgcagag atgttccctc
    20341 aatttataga aatgggtgca gtagaagatt taacacctta tttagaaaaa tcaggaactt
    20401 cagataatta tctatactta gatgatgcaa aaatgatggg tggtatttat ggtttaccta
    20461 tagaagctgc taatccaggc gtattgtact acaataaaga tatactagaa aaattaggag
    20521 aaaaacctcc aaaaacatgg gatgatttta aaagaatctg tgaaaaagca acaaaagata
    20581 ctgatggaga tggaaaaatc gaccagtggg gtcttgcaca aggttggggt tctaaagtat
    20641 ttggaagctt aaactggaac tggtatccgt atttatggca agctggtgga gatatatata
    20701 atgatgattt aaaatcagtt aaatttaata atgcagaagg cttgaaagct gcaaacttta
    20761 taaatgatat gaaaaagtat attccagaag atgctttatc aaaagatagt aatgaaatga
    20821 ttgaaagtgt atttggacca ggaaaagctg catttactat aatgctatcc tctgctgcta
    20881 caagtacatt tgataaatca ttcccggatt taaactgggg atatgtaaca tcattagaag
    20941 ataagacagt tggaagtttt gcttcagtag atgatttaac attaatgtca gcatgtaaaa
    21001 ataaagaact tggatttaaa cttcttgagt atatgttaag caaagaatca atggaagctt
    21061 tccacaaaga aatacctaga gctccagttg ctaaaggtga aaaataccaa ggagacccaa
    21121 gatttgaaga aatggtgaca agagataaac atatgtatag accattgaca gttggacctc
    21181 atggagtaga gatatatgaa tatttatgga aacaacttca aatgatgata gctggagaca
    21241 taactccaga acaagcacta aatgaatctg caaaatatgc aaatggatta ctagcacaat
    21301 aatttatatt tgaatttaca gactatataa tttgatttta tataagtaaa agatttactt
    21361 gatatattat taaattatgt agtctttttt ttataaatat accaaaagta aagcgaataa
    21421 cttctaccta gatatattgg attctacaaa aaagtctatc tgtttggcat taaaaaaata
    21481 tcttttgacg acataaagta taagacatat atcattgttc tttttaaata gaatttgttt
    21541 tggattcgac catcaaaagt gacataaaat tttgtacata ctatcttata atatgtttaa
    21601 aggttttaag gggggataca tggtgtatat tgagtactat gttatagaaa acctagtaat
    21661 aaattatatt attataagtt gtacttctat tcttactaaa agatataatc ctaaaaaaaa
    21721 taagttattg ggggctagtt taggaagtgc ctattctgtt gcttatttgt atccatcaat
    21781 gaatatacta tttactgtac catttaaaat aataatcatg agcataataa ctttaatttc
    21841 atttagaagc aagagcaaaa aagattatgt tcatgtagcg ttagtttttt atctagtaaa
    21901 catttttatt tctggaagca cttattttat aatttatttt acaggaataa gtcacttaaa
    21961 aatatctttc ttaattgcat gtacttatgt gagttgtgaa ttattaaaat acatttataa
    22021 agatttgaaa atgcttaagt acattaagga gcttacaaag actata
//
LOCUS       DAEPOZ010000037        21794 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.037, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000037 DAEPOZ010000000
VERSION     DAEPOZ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21794
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.037"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            78..725
                     /locus_tag="KR440_001142"
     CDS             78..725
                     /locus_tag="KR440_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBH0698200.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTAFKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(892..1461)
                     /locus_tag="KR440_001143"
     CDS             complement(892..1461)
                     /locus_tag="KR440_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698201.1"
                     /translation="MKKFILCIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(1466..2365)
                     /locus_tag="KR440_001144"
     CDS             complement(1466..2365)
                     /locus_tag="KR440_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0698202.1"
                     /translation="MKLLSKVNNNIRLIIQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIMFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(2376..2525)
                     /locus_tag="KR440_001145"
     CDS             complement(2376..2525)
                     /locus_tag="KR440_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698203.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(2518..3102)
                     /locus_tag="KR440_001146"
     CDS             complement(2518..3102)
                     /locus_tag="KR440_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBH0698204.1"
                     /translation="MKKFFMVVLGCIFVLGVISGCSKKESKPVEKNKSNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKNIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEEWKKIIDERLKMVKANE"
     gene            3523..4848
                     /gene="cdeA"
                     /locus_tag="KR440_001147"
     CDS             3523..4848
                     /gene="cdeA"
                     /locus_tag="KR440_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HBH0698205.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GISFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFNYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIIALVFA
                     PFIVRVFVSPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKGRYKY"
     gene            complement(4943..5737)
                     /locus_tag="KR440_001148"
     CDS             complement(4943..5737)
                     /locus_tag="KR440_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0698206.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMVIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(5738..6613)
                     /locus_tag="KR440_001149"
     CDS             complement(5738..6613)
                     /locus_tag="KR440_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0698207.1"
                     /translation="MRSSIKIENLSHSFKKQNIFNNMSLNFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEEELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSENDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(6617..6985)
                     /locus_tag="KR440_001150"
     CDS             complement(6617..6985)
                     /locus_tag="KR440_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0698208.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(7168..7827)
                     /gene="deoC"
                     /locus_tag="KR440_001151"
     CDS             complement(7168..7827)
                     /gene="deoC"
                     /locus_tag="KR440_001151"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBH0698209.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(7863..9407)
                     /gene="murJ"
                     /locus_tag="KR440_001152"
     CDS             complement(7863..9407)
                     /gene="murJ"
                     /locus_tag="KR440_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBH0698210.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILITGIIFIGITSVMSAFLQIKENFIVVGF
                     GSIPYNIVIIISIMLSTVFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKD
                     DSLIKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYSDRLNGFVTGTFTA
                     SIVSVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFER
                     GAFDARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVL
                     DLVLIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIM
                     GVLSYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMTIFKVEEVDMILNIAKR
                     KLHLKK"
     gene            complement(9881..10765)
                     /locus_tag="KR440_001153"
     CDS             complement(9881..10765)
                     /locus_tag="KR440_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH0698211.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(11056..11532)
                     /locus_tag="KR440_001154"
     CDS             complement(11056..11532)
                     /locus_tag="KR440_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HBH0698212.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGVLDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQAKDLEEHHHHDKNCGCGCNHHHEN"
     gene            11799..12974
                     /locus_tag="KR440_001155"
     CDS             11799..12974
                     /locus_tag="KR440_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HBH0698213.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(13055..13858)
                     /gene="proC"
                     /locus_tag="KR440_001156"
     CDS             complement(13055..13858)
                     /gene="proC"
                     /locus_tag="KR440_001156"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBH0698214.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINNEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(13898..14482)
                     /locus_tag="KR440_001157"
     CDS             complement(13898..14482)
                     /locus_tag="KR440_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0698215.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
     gene            complement(15368..15976)
                     /locus_tag="KR440_001158"
     CDS             complement(15368..15976)
                     /locus_tag="KR440_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBH0698216.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(16085..18823)
                     /locus_tag="KR440_001159"
     CDS             complement(16085..18823)
                     /locus_tag="KR440_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HBH0698217.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIILINICISMFNLLY
                     LLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNHTYY
                     VYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEKLKK
                     FDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKDYEN
                     IILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNIDFF
                     DNRKFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFK
                     LLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELFGI
                     SSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQKR
                     SRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQLIE
                     NLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDIID
                     ITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAIKFTLEGGE
                     ILVGIYKKDETVEIIIKDTGVGIDKEKLNDIFNRFKQIENSGISNEFGSGIGLCLTKS
                     LIEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK"
     gene            complement(18971..19762)
                     /locus_tag="KR440_001160"
     CDS             complement(18971..19762)
                     /locus_tag="KR440_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH0698218.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(19798..20454)
                     /locus_tag="KR440_001161"
     CDS             complement(19798..20454)
                     /locus_tag="KR440_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0698219.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(20447..21412)
                     /locus_tag="KR440_001162"
     CDS             complement(20447..21412)
                     /locus_tag="KR440_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HBH0698220.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEHVKQYLTSKGIRWEEMINE"
     regulatory      complement(21518..21624)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT         7250 a         3579 c         2266 g         8699 t
ORIGIN      
        1 atacaataat atatcgtata atcttaatta gcataatagt tacaatatta aaatttaaat
       61 atgggagtga ttttagtatg ataagacaaa caccattaga gaaacgatat gacaagctag
      121 gtcctcatat tgtaacagca ttcaaaaaac gttattttga tgcttattac tgtaaaacta
      181 gaaatgaagc tttgcaacaa attttagatt taattcctca aaatcatgta gtatcatggg
      241 gaggatctga aacacttaaa gaaatgggcg tacaaactgc tgttagagag aaaggattta
      301 aagttattga tagagattta gcaaaaactc cagaagaaag aactgaaatt atgcgtcaag
      361 cacttctatg cgatactttt ttgatgagta gcaatgcaat tagtgaagat ggacaactct
      421 ttaatattga tggaaatggc aatcgtgtag ctgcaatgat atttggtcca aaaaatgtta
      481 tagttgtagc tggtatgaat aaaattgtaa aaaacattga tgatgcaata caaagaacta
      541 gaacaattgc atctccagca gttgttcagc gttttcaaga tgtcaataca ccatgctata
      601 ttaatggaag ttgtatagat tgcacaagtc ctgaaagcat ttgtgcatac atggttacta
      661 caagaatcag tcgtccagca aaaaaaataa aggtaatatt ggtaggagaa aatcttggtc
      721 tataaaatat acatacatgt aaatagctct ataaataata aaatcatatg aaataactta
      781 tttctaaaag tggtagaata aaagcttcta caagactttc attctaccac ttttatattc
      841 ttttaatttg tgtttctata ttattaattg ctccaatttt catatatatt actaactatt
      901 tttattttca taattttttt gttcttcatc aagctgttta taataatctt tattatagtc
      961 agctagataa tctataccac ctcctgtaat cacatttaat aatttcattg cagattcttg
     1021 attaacttta caaacataat tatcaccaga tttaacaagc tctaatgtaa tattaaattc
     1081 ttttttcttt gcagaatcaa tatctttcat agttggattt ggcatcattt ctcctagttt
     1141 aggaggattc ttttttaaaa tagaaaccat atcagtattg ttaattttta aatctacagt
     1201 cactttatcc ttattattct ttatatctga aatttcccaa gtcatatttt tagaatacat
     1261 tttaaatcct tctttatctt cgtatataag attacctaac cctgattttg ggaaatactt
     1321 atcaacagta tctgcatctt gtgtttttat tgaattaaac atactatcca cttcattttt
     1381 tatcttatta tcattagtac aaccagctaa caccagtact gatattgcaa acattataat
     1441 acatagaata aatttcttca ttaaattata tccccctata cttttttgca caaatattat
     1501 tctctttttt atcttttgaa aaagttgtag taattgcatt ttgtggacaa acttttatac
     1561 attcaccaca cctaatacag tctggactat ttacatcttt atatattggt atatcaagct
     1621 tacattttgt ttgacatgct gtacaatttg tacatttatc tttatcaatt ttaaatctat
     1681 atattgaaat aggattaaaa aaaccataga ttgctcctaa agggcatata tacttgcaaa
     1741 atggcctata agtaatgatt gacatcaata atatcaatag caaaatactt aacttccagc
     1801 taaataaaaa tcctattgat tctcttaaat tctcatttcc taaaataagt ggtattcctc
     1861 ccaaaagtgt tcctgaaggg caaatccatt tacaaaacca aggatttcca ccccctgcta
     1921 catttacaat agcaactggc aaaataatta caaacattat taagactata tactttagat
     1981 attttagata tttatcacca taaacttttt ttatctttac tggaaagggt actttatgta
     2041 gtaaatcttg tacaagtcca aagggacaaa gccaaccaca aacaaatctg ccaaaaaaag
     2101 caccaaatgc cattaaaaaa ccaacaacat aaaatgagaa tttatactct ctgcttgata
     2161 aaactgcttg taatgaacct attggacatg ctccaagtgc acctggacat gaataacaat
     2221 tcaatccagg tacacataat tttttacttt ctcctccata tatagttcct tctaaaaatc
     2281 catttacata tccatttgtt aatgctgtaa atgcaacttg aataataagt cttatattat
     2341 tatttacctt agataataac ttcattcctc ttctcctaac caataccaat acactcaaga
     2401 caaatcataa ttgctttagt taatactgta tttgtttctc cacgatatgt acctaatgca
     2461 ataaatataa tactaattgc aataatagaa atgccttttt tattatcttt aataaagtta
     2521 ctcattggct tttaccattt ttagtctttc atctataatt tttttccact cttccttact
     2581 atttgcacct acaaccttct cacccacaaa gtttcctttc ttgtctacaa aataagttgt
     2641 tggaaactcc ataactcttt taaaatcagt ttttttaagt tcttcagata cagtcaattg
     2701 ttggtaagtt gcatctgact ttttcataat gtttttaact aaatttttct cttcatttga
     2761 aagaccagtt ctcatatcag ttttatcaac ttctacaact aatcccttta tagctacatt
     2821 ggaattatta ctctcatatt cttttgataa tgcttcaagc tctggcattt cacccacaca
     2881 tggtccacac caagtagtcc agacattaat aagagtaagt tctttatttg aaaatatctc
     2941 ttttgttacc ttatttccat caacatcctc cgtatctaaa ttactaaacc attcactatt
     3001 actcttgttt ttttctactg gcttagattc ttttttgcta catccagaaa tcacacctaa
     3061 aacaaaaata catcctaaaa ctaccataaa aaactttttc atatctcaca ctcctagaat
     3121 ttatatagta tataaaatgg ctgcttgaaa taaaaataac ttctcttcta tattaaaaca
     3181 ttatatttaa taaataagaa ctaatttaaa ataagttgta taaaagatat atagttaaaa
     3241 tctatttctt ttcagacaaa atcttatata tatataataa cttataaagc ttaagattat
     3301 atacatatat ttctaaaaat tttcttaaga aacagttatt ccttactaat gcaatatacc
     3361 tatttttact aatataagtt tattaattag cattatacta atttatctta atatgatttt
     3421 ttgttgattt taagctgttt atcgtataaa taaaattttt cttgacatat agttactata
     3481 tagcttatta tagatagaga gtaataaaag gaggtaaaat ttatggaaaa tttatttaca
     3541 agaaaattca ctacttttga atttctaaaa tttgtttctc ctgcaattat atccatgata
     3601 tttatatctt tgtacacaat aatagatggc atctttgtat caacattagt tggttccgat
     3661 gctcttgcta gtataaatat tgtactacct ataattaacc ttgtttgtgg atttggaata
     3721 atgatggcaa cgggtggagg tgctatcgtt tctatacgta tgggtgaaaa tagacaggat
     3781 gaagccaact ctacattttc ttttatagtt ttgttttcat tgattgttgg gatttcattc
     3841 acagtaatct catatttctt tatcaaagaa atatctattt tgcttggtgc aacagataag
     3901 ttattaccat attgtataac ttatggtaaa gttatgattt tatgtacacc attttatatt
     3961 ttaaaattta tatttaacta ctttgcaaga actgatggaa attctaaatt tagtttattt
     4021 ctatcagtca ttggtggtgt aacaaatata atattggatt atgtatttat taaatatttt
     4081 ggaatgggtc ttttaggagc tgcagttgca actgctatag gtattatttt aacttgtgtt
     4141 ttaggtatta tttacttctt atctaataaa tctacactca aattaagaaa accaaaaacc
     4201 gattttagac ttataagaga tactatgata aacggttctt ctgaaatggt tacagaatta
     4261 tctacaggaa ttacaacatt cttatttaat gtagtagctt taaaattagc aggagaaaat
     4321 ggacttgctg ctcttactat agtattgtat gctcattttt taatgacatc agtctatcta
     4381 ggattcgctg ctggagtgtc tccattaata agctataatt ttggtgctga aaacagtgat
     4441 aaattaaaag aaacatttaa acattctcta aaatttatat ttgtttcttc tcttttagtg
     4501 tttattattg ctttagtatt tgcaccattt attgttaggg tttttgtaag tccagataac
     4561 acagtattta aactagcctt acaaggatta aaaatatttg catttgcttt tttgtttgtt
     4621 ggtataaata tatttgcatc aggatttttt acagcatttc acaatggaaa aatttcagct
     4681 attatatctt ttagtcgtgc ctttgttttt ataatcatag gaatcataat tcttcctcct
     4741 atgttaaaca tgactggatt atggcttaca gttccatttg ctgaggttat aaccatattt
     4801 atatctattc tatttataaa aaaatataaa ggtagatata agtattaaaa attaatgctt
     4861 aaaatcaaca tttattaaaa ctaataaacc tgctttaaaa tacaaattta caaatttgat
     4921 ttttaagcag gttttatttt agttataata agtcatttgc atactcttta attggtgctt
     4981 ttctaagtaa taaaattata aatattatgc aaataactat aattcctacc tttgttccaa
     5041 ataaaataaa aggattctga aatgaataaa taattttttc taaatacaat agcattttat
     5101 gcatattttt agttgcaaaa ttaactatat aaattaccat taatatcggt aatcctatca
     5161 atatagtaat actagtaact tttctaactc tatatgtaaa agctccaagt aatactccta
     5221 taaaacctat acatatttca tttatacata tttgtacaaa ataatttaaa taagttagtg
     5281 gcatacttga tgttgaagga tctatcagtg ctgatataga tgtccaaatc atatctctct
     5341 ttagtaaaac tgatagttct atactgtaac ctgttataat ttctataaat aacttcgata
     5401 aaaaaatcaa aactatacct aaaatagaaa tcacaaagct taaaataatt aaatctatga
     5461 caatagcttt tatacaactt tttcgatctg ctcttatact taaagctcct gaaaagtctt
     5521 tatttgctat atttactcca tacacaaata gcagtatagt tatatatgtt aaaataggat
     5581 tcacaatcgt ggataaatta gttttagatg cttctccaaa aatcttgagg ataaacactg
     5641 gcaaaatatt cataacaatt cccacaatca taaatataat tatatacaat ttagtatttt
     5701 tattaaaaaa ttttatatat ggctttaatt cattcaatta caattcctcc ttcttagtca
     5761 tgttaacaaa catatcttgt agccctatgt atcctacatc aatatttgaa tttttaagta
     5821 ttttttcatc attttcacta aaatcaccat agtaaaaata agcaactgca tttccaaatg
     5881 cctttttagg ttttatattt tttatacatt ctaatttttc taactcttct ttatttccag
     5941 taatataatg agctttttgt tttatgacat caatttcttc atctataata atttttccct
     6001 catttaatat tattacatgc tccagtaaat catctatttc atcaatcaaa tgagttgata
     6061 ttattattgt ccttggattt tttatataac tatctaaaat tatgttataa aactcttgtc
     6121 tatttactgc atccaaacca attgttggct catcaaatat ggtgatagct gaattagaac
     6181 aaatacctat tatatttgaa agtatagttt tcatacctct tgacagttgt ttatactttt
     6241 ttttagtatt taaatcaaaa tatttgcata attcttcttc taagccttta tcataacttt
     6301 tataaaaact agaagaaaat tcaaatatct gacctacttt aaaatctgga ctataaaatt
     6361 ctttttccct gacgatacaa acttcttcca aaacttttat atcttctttt gggttctttc
     6421 ctaatatttc tatctcacct tcattttgaa ttaactgatt taccattata tttaataatg
     6481 ttgtttttcc agctccattc ttacctaata aaccatatat cttatttttt tcaaagttta
     6541 gagacatatt attaaaaata ttttgcttct taaaactgtg agataaattt tctatcttaa
     6601 tagaactcct catatattaa tcctccttga aattaattat aatatcaacc aattcttctc
     6661 tatttatttc taatttgtta gcttcttgaa gtaggttttt tacaaaatta tgtttaaaat
     6721 tttcttttct agcttctttt ataatagttt ttgcaccatc tgatacaaac atccctatcc
     6781 ccctcttctt atataaaata tttttatcaa ccaatatatt tatacctttt aatactgtag
     6841 ctggattaat tttataaagc tttgaaagct ctgttgttga tggaacctgt tcttcttctt
     6901 ttattccatt tgatagtatc tcatcttcaa ttgctcgtgc tatctgaata aatataggtt
     6961 cttcattatt taatactaat ttcataggtt cctcctttct ccattagtta gttactcaac
     7021 taattaacta tataacttat aatatcttga aaaatatttt ctgtcaatac taaataaaaa
     7081 acagagggaa aattccctct gttttttgtt ctttatattt ataattaaat tttgattttt
     7141 gtaatcctat cccataagtt aaaaacttta ataatctgtt gtatcttcat tttttaatat
     7201 tttacatatt gagctagttc ctaacctttc tgctcctgcc tctataaatt tttctgcatc
     7261 agaaattgat ttaactcccc ctgcagcttt tatttttaca ttttttccaa catgctcttt
     7321 cataagcttt atgtcttcta aagtagctcc acctgttccc attccagtag atgtttttat
     7381 aaaatctgaa cctgacattg ttactatttc acacattttt attttttcat cttcatctaa
     7441 ataacatgtc tctataatga cttttaatac tttatctcct atagcttttt ttatttcttt
     7501 tatttctttt cctatattat cataatcttt attttttata tctgaaatgt ttacaaccat
     7561 atcaacttca tctgctccat tttcaatagc atcttttgct tcaaatactt ttgtagcagt
     7621 agtttgatat cccaaaggga accctatcac tgtacatatc tttattttac cttttagata
     7681 ttctgatgct cttttcacat atgatggtgg tatgcaaact gatgctacac tcatatctac
     7741 agcttcatca cataaaattt taatatcttc ccatgtagtt gtagctttta aaatagtatg
     7801 gtctactgtt tttaatatat gtttcataaa atcctcctaa atttataatt aatataatta
     7861 gattactttt ttaaatgcaa ttttctcttt gcaatattca gtatcatatc aacctcttca
     7921 actttaaaaa tagtcattag tagtgtataa attccacctc caactataac tgaaatagct
     7981 agtgatacaa actcattaaa tgttcctact cccaaaattc caaatataaa tttatacgta
     8041 aagtatgata aaacacccat aattaaagat gcaactaccg atttcaaagt tgcttttatt
     8101 attttatctt gtccaaaata tccaactttt ctctttaggt tcaagaagag taacaatata
     8161 catgcaattg atgatgaact agttgcaaga gcaagaccac catgagccat tggttttatt
     8221 aaaactaagt ccaatactat atttacccct acagatataa tcccatttac cataggtgtt
     8281 tttgtgtctt gtaatgaata aaaaaccttt cccaaaatat ctcttaatcc aaatgcagtc
     8341 ataccaactg cataaaaaat taaggcagtt gctgtcattt gggttgccct cgcatcaaaa
     8401 gcacctcttt caaaaatgat tctaacaaca ggagttgcaa aaaaaataga agcaactgaa
     8461 ataggaatca tagaaattat tatcatattt atactagatt taactgaact agtaaacttc
     8521 ttttgattat tttctgcact taacttagat aacataggat acataacaga tactattgaa
     8581 gctgtaaatg tccctgtaac aaaaccattt aatctatctg aataagtaag tgctggaatg
     8641 cttcctttaa ctaatgttga ggctaaagtt gtatctacca atgagttcac ttgattaaca
     8701 gcaacaccta taaatactgg tgaaagtaat gctagtaatt taataaggct atcatcttta
     8761 aagtttaagt agtataagta tttgtagttc gtttttttaa caaaaaacat gtaaaagagc
     8821 agttgaacta ccatagcaac cacagcacca attggaagta tgtatggtcc aaatacggta
     8881 cttaacatta ttgaaataat tattactata ttatagggta tactcccaaa tccaactaca
     8941 ataaagttct ctttaatttg cagaaatgct gacataacac tcgtaatacc tatgaatatt
     9001 attcctgtta tcaatatttt agtaaatttt acggtaagta aaaacctctc tccttcaaat
     9061 cctatagcaa atatacttac aagttgcttt gagaaaataa ctcctaatat agctacaact
     9121 atgcatatac caactattat atttaaaaca ttgtttaaaa actttagtgc ttgcttttca
     9181 ccttgtttac tacttatatc ttgatacatt ggaataaaag tagtaactat agcagtacct
     9241 attgctgcaa atataatatt tggtatattc atagctgtta aataactctc tgtatataag
     9301 cctgtaccat aaattgatga taatactaat tctctaccca ttccaagaat cttagagatt
     9361 atagtaacta tcatcaaata aaaagttgct ttagctacct tgctcatttt atcactccac
     9421 ttctaacata cctcttttta atcaagtaaa atttggctca ttagatataa ttctatcaac
     9481 taatttgcct tgcttagccc tatcaaaata taattaacac ctatttctta atatatcaca
     9541 aactacatta ttcttcatat ttttttattt ctccaataag ttttacatct ttcctatata
     9601 aattgtatag cttcaattat attcaaagaa atggagttgc tacccgataa ctttaaaagt
     9661 aaaaaacaga ttttattatc tttttaaact atattctaac atattttgtc catattgaga
     9721 aattttacta aactattatg tactttatta aataaataaa ataacagcac acctataaaa
     9781 tacaagtgtg ctgttcataa acagatttta taaaaaaata attcctaagt agagattaac
     9841 atgctttctc atactctcat atataatgtg tctaaaaata ttaataaaaa tgctttaatt
     9901 gttttaactt acttggatgt cttaattttc ttattgcttt agcttctatc tgtcttattc
     9961 gttctctggt aactccaaat acctttccaa tctgttctag tgttttaggg tcatcatcat
    10021 caattccaaa tctcattctt aaaacttgct gttcttgctc accaagacct gtaagtagtt
    10081 cttctatctt ttcttttaaa ttatgctgtt caacttcgtg tattacaaca tctttaaaat
    10141 cagcatctga tggtataaat tcaactaaac tactatcctc atcttctttt aatggagtat
    10201 ccaaagatac aacattttta tctcttctta ataattccaa cactttgtca acttctaagc
    10261 cacatgcatc tgcaatctca tctatagttg gctctcttaa taatacattt aaaagttctt
    10321 gtttcttttt ctttacaaaa tttattctct gatgaagatg tataggtatc ctaatagtat
    10381 tttcgcaatc gtcgatatat cttgttatac tttgttttat ccaccaagtt gcataagtgc
    10441 taaatttgta tcctttttta taatcgtact tttctacagc ttttataagt cctatatttc
    10501 ctgcttgaat taaatccaac atatcaatac tatctctttt atatctcttt gctatgctca
    10561 ctaccaacct ataatttgaa tttataaatt tttcttttgc tactgaagat gagttgatta
    10621 tttccttagc cagctctacc tcttctcctg ctgacaacat tttgtattcc tctatttcct
    10681 ttaaatacat tttcatcgca tttgatacat ttgatgaacc cacacaaata taattttcat
    10741 aattattttc ttttttagta tccataatca aaccccttct attcatagaa tttgctaaaa
    10801 caaaaataaa taatttaaac ctacataatc tacaattaca taatatccca ttctttaaat
    10861 gaattgtttt gtatattatg tatacaaatt tataaaaact atacctataa tattattcta
    10921 cttctaaccg caatatcctc cttatatata taatttattt tattatatgt tgttatattc
    10981 ttagtttttt gaatgatata tgtctaaaaa aagactgttt actaaacagt ctttttttat
    11041 atatatgctc aatatttaat tttcatgatg atgattacat ccacatccac aatttttatc
    11101 atggtgatga tgttcttcta aatcttttgc ctgttgttcc atttcatgaa actcttttac
    11161 cattatatca taagttggaa gagctgcctc agtatcgtat tcttcccctc tccaatcagc
    11221 gtacatcata gcatccccat tagttaaaac caatctacct gacatagtta tattatccac
    11281 ttcttctaaa aattctattt gtttttgtaa aacttctcct tctaactcta catcttcatc
    11341 taaacattgc actatcatta caggtgcata ataatcttct tcatctttaa catttacaac
    11401 aatatctaaa actccttctg caccttcttc aaattctgcc aactcaacat ttgtatcaat
    11461 catatcttga gggactaata aatcttctat taaatatttt attactctat cttttactaa
    11521 ttcttttgac atttattttt ctccttccga ttctataaca ttatcataat catttctata
    11581 ttatagtata ctacattaat tgttttttca atctcttaca aaaattattt ttttattatc
    11641 ttccttatat tccctttttt attacaaagt tgtaatcgca ttgatttaaa aaatagttta
    11701 tatttagtat aatagaaatg tagcacatat ttttattcaa tatattaata aaaaaataga
    11761 ttgaaaatgt aatgtatgtt aaatctagga ggaatattat gaagtttaaa aaacaaatta
    11821 gttctattgt tgtgtctact atgcttgtac ttgggagtat gaacctttct tatgcagatg
    11881 gtacaaatgt agttacactt ggagctaact tatcagagtc tcaaaagcag caaatgctta
    11941 attactttgg agtaaaaaaa gaccaagtct tagttttaga tgtaacaaat gcagaagaaa
    12001 gacagtattt acaaggtgtt gcaacagagg gtcaacttgg aaaagttact atctcttgct
    12061 catacgttga acctactaaa aaaggaaatg gaattaatgt taaaacagca aacttaactt
    12121 gggtaactag ttcaatgatt gcttcaaccc ttacaacagc tggtcttgaa gatgcaaatg
    12181 ttattgctgc tgctccatat cctgtaagtg gtactggagc attgacagga atcatgaaag
    12241 catttgagga tgcttcagga aagaaacttg atgaaactaa gaaagaaatt gcttctgaag
    12301 aattggtagt tacaggtgac ttaggagatg atatagggca agaaaaagca acaggtgtaa
    12361 tgaatgatat aaaaacagaa attgtaaaga atggtacaaa agataccaat caaatagcaa
    12421 atacaataaa taatgttgta aataattaca atattactat tacaccagaa caaaaagaac
    12481 aaattaaagg tgtaatgaaa aaaatagctg accaaaatta tgattacaat agcatgaaaa
    12541 atacactaga taatgtatca gataatgtaa atgaacaatt aaagaaatta ggtgaaagtg
    12601 ttaagggttc tggtatacta gatactattg gtggatggtt tagtggaata ggagactggt
    12661 tctctggttt attctcaggc ggagataaaa aagatttggg aatcttaaat aacactaatg
    12721 atgagtctct tggctctgat gctgtaataa attctacaga aggtgttcaa ataaatccac
    12781 aaaataatac taattctaac aatgatagtg atacaaatag ctcaaaaaat gatacttcaa
    12841 gtaataatga aaataacgat agttctcaaa caaatgaaaa caacacaaca aataattcag
    12901 aaaatcaatc aaataataat gactcagttg gtttctttga aaaaataaaa aattggcttt
    12961 caggcttatt ttagaaatta aacaaataaa aagaattgcc taaaatggaa attttctata
    13021 aaaggcaatt ctctttattt acaataaatt tttattactc actcattttt ttagattttt
    13081 ctatacatgc acatattgct tttatcacag aagctctaaa accttcttct tcaagtactt
    13141 taactgcttc tatagtagtt ccaccaggtg aacataccat atcttttaat tcaccaggat
    13201 gttttcctgt ctcaagtacc atttttgcag aacccataac agcctgtgat gcaaacttat
    13261 atgcttgtgg tcttggcatt cctgcaatta ctgctgcatc tgctatagct tctataaata
    13321 aaaatacata tgctggtgca gaaccacttg caccaattac agcttctata agatactctg
    13381 gtactacctc agtatttcca aaagaattaa atacttctgt aactgcttct aagtcttcat
    13441 tgtttatatt tttatttata gaaagagaag acatggcttc atttacaaga gctggagtat
    13501 ttggcatagt tctaactatt tttttatctt ctcctataat tgactctata tcctttatag
    13561 attttcctgc tgctattgta actataattt tattgtcatc tataaagtct tttatctctt
    13621 ccaaaatatc atcatatata tctggtttaa cagctacaat tactatatca gaggttctag
    13681 ttacttcctt cgagtctttt gtagtattta ttccaaaatt tgcatctatt ctatcgagag
    13741 ttccttgact atatgctgat gctataacca tattaggttc aactaatttt gaatttacta
    13801 ttccccctat catagcgctt gccatatttc ctgcacctat gaatccaatt tttttcactt
    13861 tagtacctcc ttattgacta atagtaatcg ttaaaattta agcttcttta actgcatcag
    13921 acttttcaaa tggatttggt atacttacta ccaaaatttt cactacctta ttagtatagt
    13981 tgacccaatt atgattgttc ttttcactat tatagtgtat tgtgtctcct ggatacattt
    14041 ctatttgttc atctcccaaa aacactgtaa gagtaccctc taatacatac acaaattcct
    14101 caccttcatg tacgtaacaa caaatttcct cactactctt acttggaaga atttcaatta
    14161 atcttggtag cattgttttt tcctttaaac tagatgataa atgataatgt ataaatgttg
    14221 agttttctac atcaaatact tctttttcat aacttcttaa tacagctctt ttatgctctt
    14281 ttggcttcat aaaaaagtaa gttaattcta catctagtac acttgctatt tttcctaagg
    14341 aatctgttgc cacacttgtt aatcctcttt caagttgtga taaaaaacca attgacaagt
    14401 ttgttttttc actcatatcc ttcagcgtca tttgcttttg agttctgagt tgctttattt
    14461 ttgctcctat gtccttattc aaaaccatca cccctaaata attttaattt tttcataata
    14521 taaatacaaa aatataaata taggtactga acctatcttt atttcattta aaaaaaattt
    14581 ttataagtat tactataatt tatcaatctt tttttgtcta catttttacg attatttacg
    14641 atttattttt cacatatgta tatttaatct tgccaactaa agatacattt tttataagca
    14701 tttactcttt atataataaa acttaagttg ttcatgtagt cttcttctgg tttaattatt
    14761 aaaatttcaa attatattat tatttctaca cattattaca tcctttagta caattataac
    14821 ataattttac aatttaatga tatctattca atgtcttttt ttactctaca acatatttta
    14881 ttttagagtt taatactata tatatttttt atatacagcc ttgtcatttt ttagattgtc
    14941 aagagttatt gaagtatttt atgtagattt atattttatt ttctaatttc tcatttaaca
    15001 agaattgctt atgataaaaa ataatatcat ctcaataaga ttcttgtaaa tatatctata
    15061 ttctaaatat aatttctttt tttgtcaaaa tttcttcatt ctttaataat taatttatta
    15121 cttataaaat aaactaatta tctatacaaa ttttataatt tttaataaaa tacacaaaaa
    15181 aattaatttt tatagctcat ttcgccaaaa tatatctttc tttatatcat taaaataatt
    15241 ataaatttat taaatatgtt tgtaattcta taaagttatt tttatcaaac taaaaattct
    15301 ataaaaaaat aagatacata cctataaaat accttcctat agatacatat cttacctata
    15361 taaaaactta ttctatataa aaacgccata aaaaatcttt tgcttcttct gcataatcta
    15421 tatttatacg tttactagtt tttatatcta actcatcttt actaaatact ttttttcctt
    15481 tatcatcata ataaaaatca cttatatata attcttcacc cattatactc ttactattta
    15541 gacttctatc tatttttaaa gctttgcaaa gttttcctgg tccgttagta atatttttta
    15601 cttgatattt actcaactca gtgtaagctt tgttgtatct attcatagat atctcatcta
    15661 aagaagttat aggctctacc cctcttatta atacacactc tggcacattt tctatatttg
    15721 cagataaatt taagcaatta tacataccat aaattaaata aacatatata tgtccaccat
    15781 ctaaatataa tggcttagtt ctatctgtct ttttattgcc atatacatgt gctcctttat
    15841 catttacacc catgtatgct tctgtctcta tgattttggt aacaattact ttattttcat
    15901 atttccttat tagatattta cctaatattg atttggctaa atcaattcca ttttttctaa
    15961 aaaaatcctt ttccaaaatt tcacctcata aaatttattt aaaacgcatc aaatacaact
    16021 aaagtaataa ataaaattaa ctcttattaa tgtgatatat ataaacaata aatttagcca
    16081 tttattattt atatatatca aaaaattcta tctcaaactt ttcaatatta tcattgtaat
    16141 ttgaattatc atatacttct tcaacaacct ctttaattgg gaatataact tttacattag
    16201 ttccttctcc taccttacta tctatgtaaa tcttcccatt ttgaatttca atcaatgatt
    16261 ttgtaaggca taaacctata ccacttccaa attcatttga tataccactg ttttcaattt
    16321 gtttaaatcg attaaatata tcatttaatt tttctttgtc tatgcccact ccagtatctt
    16381 ttataattat ctctactgtt tcgtcttttt tatatatacc tacaaggatt tctcctcctt
    16441 ctaaagtaaa ttttatagca tttgataata tatttagtat tattctttct atagattctg
    16501 ggtcaagccc taccataaca gtttcttcat ccgtatcaaa aattaaatct attcctttag
    16561 acttagcata actactaata gatgtaacta ttctttctgt tatgtcaata atatcaaaat
    16621 taactgcttt aatcttatat atgtctgact taacttgaga aatatttatt atattatcta
    16681 ttaatctcaa taatctaaac atattttgtt tagttatttt tatatgttct tttagtatat
    16741 cctgaaaatc aattattttt ccttttttat aaaggttttc aataagttga actgaactat
    16801 atatattatt taaaggtgtc ttaagttcat gagatatatt agcataaaac tccatcttca
    16861 tttggtcata ctgtctttgt tttattaatg cttttttaag tctttcagac tctctttgca
    16921 cacttaaatc tttaataatt ctcactctac ttcttttctg tccaaagtat aatgttcttg
    16981 atatcatttt tacttcttta atgtgattat tttcatttac tatatggata ctcttctctc
    17041 tttctcctat atttaaattg tttatgtatt tttttactaa tatatttcta tatttttgtg
    17101 ttgtaactcc taatacatca ctagaaattc cgaacaactc tctaaaggct ctatttgtat
    17161 atagtactct tgatgtactt aaatcctcaa gaaaaatagc ctctggtata acatcaagta
    17221 atttttcata tattattttt tgttcattta ctgttctaag aatatttatc tctttagtta
    17281 tatctctaaa tgtaagcaat ttaaagagtt tattatctga ccaatagtct ccaactctaa
    17341 cttcttcaac atgttcttca ccttctgagt cagacatcac aaaattaact atacagtttg
    17401 ctgtgtcatt gtaacttact atttcagctt tcttttcttg tgttagattt aatcttttaa
    17461 atttcctatt gtcaaagaaa tctatgttaa aagtttcaaa atattgttct aatgtcatat
    17521 cgactaaatc acttgaaaca catttaaaac tcttttcata ttttttattt atacttatta
    17581 ccttattctt ctttatgcca ccttcagcac ttcttacaag tataggagat ggaatattta
    17641 gtataatgtt ttcatagtct ttatttatat ttcttatttc ttcatctatt ttgatttcat
    17701 ttgtaatatc atttaaactt attaagtaat tgctttcatc tttattatta cttaaactta
    17761 acaaataaat tttatcattt aactctacca gtttatcaat tatacttata tcgttatcaa
    17821 atttcttcaa tttttcagca actttttcat cttcctttat aaaagttaat attttataat
    17881 aattattcat atcctttgaa aattcatctt taaatttatt atttgtgtaa cttatattaa
    17941 aatcatagtc tgttactatc catacatttg gtattgtatc cagagcttgt ctaagtaatt
    18001 cttttctctg gtctatgtag ttttttaatg ttctttgtat aatcatatcc ttttctattg
    18061 ctaaatttat ctttcctaat tgctctgact ttttacttaa ttttttattg attgaatcaa
    18121 gttcataata tggatacact actctattat taagactgta tacatataca taataagtat
    18181 ggttacataa tagtaaatat attactccta ttaatctatc tgaatctact atattaatct
    18241 tagaaattgc tacaaaacat ataatagttg taaaaaaaac aaaatatgtt aaatctttta
    18301 ttaacttata gtgctcaatg ttactatcaa gtttattcaa caaatatagt aaattaaaca
    18361 tagatataca aatattaatt aatataataa tcatattcca gttaatatca ctaaaaatat
    18421 ttcttataaa gtaatctaat atcacaaaag cactataaaa aattagtatt gggcttattc
    18481 ttagtttttt tactttagaa atggttaagt atataaaagt agacatatta attatgctta
    18541 agttaagcaa tacaatttgg aatttttgaa tttttaaatt tactactcct aaaatactta
    18601 caaaaactaa tacaagacac cctactttca atatatttat aaattcatct gttttaaagc
    18661 tacttatata aactatagaa tatccaaata tccaaagtaa aattattcta tacatattta
    18721 tagaaactgt atacaaattt atatttaaaa gtgcaataac caaaaataac ccattagcta
    18781 gtatatagac cttattctta cttaaagttc ctttagattc catattcccc accacttctt
    18841 catttttata tagttttacc tataaatatg ttaccacaat tcttccaaaa aatttgtcac
    18901 tttctagtta taaaaaaaaa tgagtaaata tttttaattt tactcatttt gtagattttg
    18961 tatattcagt ttaaaaagct ggaactatac ttcctttata ttcatcttgt ataaattttt
    19021 taacttcttt actattcata gcctctgata aagccttaat cttatcacta tctttattat
    19081 tttctctaca agctattata tttacatatg gagaatcact tgattctatt actattgcat
    19141 ccttagtagg atttaagtta gctgttaaag cataattaga atttattact gctccgtcaa
    19201 catcttttaa tactgttggt aattgctctg cattcatttc ttttattact atatttttag
    19261 gatttttagt tatatctttt ttagttataa gctctccatc ttttacttct atcaatttat
    19321 tttttgctaa taatttaagt gctctagctc cattagttgc atcatttggc acagctataa
    19381 cagctccatc ttttaattca tctaatgact taactttttc tgaatagaat cccattggct
    19441 ctatatgaac tttagatgta taagtaagtt tatatccttt ctccttcaca gtttcctcta
    19501 aaaatggaat atgttgaaag aagttagcat ccaaagaccc ttcatctaaa gctgtatttg
    19561 gaagtacata atcatcaaat atttttactt ctaaatcata tcctttttct tttaacaaag
    19621 gttttgcaac ttccaatatc tttgcatgtg gatttgatga agctcctact agtatttttt
    19681 tatcctcttt gtttgaacaa cctactgctg atattgctat tgctgacacc aatgctactg
    19741 ataataattt ctttaatttc atttttactc cccctcttta aaatgtttat aattaattta
    19801 ttttagtttc ttataagcca gatttcctac tccctggatt atctgaacta atattataag
    19861 tgctattaca gtatatacca ttactgctgt atcaaatctt tgatatccat aaataagtgc
    19921 tatattacct aatcctccag ctcctactgc acctgccata gctgtataac caagtataga
    19981 tattattgat aatgtaattc cagatactat tgaaggcata gcttccttta tcataacttt
    20041 aaatactatt tgtctttttg tagctccaaa agattttgct gcttctatca atcctttatc
    20101 aacttcattt aatgagcttt ctataattct agcgataaac ggtgctgccc caatagttat
    20161 tggcactatt gcagcagttt cccctatact tgttccaacg attaatctag ttattggaat
    20221 cattgcaact ataagtatca taaatgggaa acttctaaat atatttacta taaatcctaa
    20281 tgcactattt attgtgctat ttggttttaa tccatctggt tttgtaacta ctaatatgat
    20341 tgctaatata aatcctaata ttgttgccac tatagttggt actataacca tatacaaagt
    20401 ttgtaacaat gcatttggaa atagtgttgt taaaaagtca attaaactat tcatttatca
    20461 tttcctccca tcttatgccc ttactagtta gatattgttt tacgtgctct ccacttttat
    20521 ctgaaatgtt tataataagt gaacctaaaa tatcatcttt aaattgttct aatttcccaa
    20581 atattataga cacatctata tttagttctc ttgccatagt cgtaataata gcttcattag
    20641 aaatatcttt agggaatagt atctttatat ttgttccttt tggcaatatt atactttctt
    20701 cacctattaa ttttctaagt cctttagtat ttcttaagaa tatttcttct gtatctccta
    20761 cctctaatac ttctccattt tccattattg ctactcttcc acaaatctgc tttataactt
    20821 ccatttgatg agttacaact attattgtta ttcccaattt tttatttata tcttcaagca
    20881 atgaaagaat tgactttgta gtatttgggt ctaatgctga agttgcttca tcacaaagaa
    20941 gtacttgtgg gtttagtgct aaagctctag caattgcaac cctttgtttt tgtccgccac
    21001 ttagatttct gggtttatca tttttctttt cagatattcc aactacttct aataactcta
    21061 aaactctttt tttaatttct gcctttgagt atccaaaaca ttctaatggc aatgctacat
    21121 tatcaaatac agtttttctt tcaagcaatg aaaagtgttg aaaaatcatt cctaactctt
    21181 ttcttaaatc tctcatttgc ttttcattca atgactttac ttctttatca gaaacaagta
    21241 cactgccttc ttggtattct tctaatccgt ttatacatct cagtagtgtt gatttccctg
    21301 caccactatg acctattatt ccaaaaattt ctccagattc tatttcaata cttacatctt
    21361 taagcacttg aatctttcca taatacttat taacattttt tatgctaatc ataaaatttc
    21421 ctcccatata ttctgctcag tttttatgtt tatcctctct tatgtattac aacaaaataa
    21481 aagcccgagt ataaactcag gcttagaaag ctatttatac tcatctatca actatattag
    21541 ttgcaggatt tagcaccaat gtatataaaa tatactggtt gccgggtttc aaagggccag
    21601 tccctccacc actcttgata agacgttatt taattcaatt ctattatcat ttgttgctat
    21661 gttatatatg atatattcat taatagtatt tgtcaataaa tttatttaaa ttttttattt
    21721 tttattatcg ttaattaact atttttaatt agttttctag ctcctaacac tttatatttt
    21781 aatgtctata taac
//
LOCUS       DAEPOZ010000038        21573 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.038, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000038 DAEPOZ010000000
VERSION     DAEPOZ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21573
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.038"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..2563
                     /locus_tag="KR440_001163"
     CDS             <1..2563
                     /locus_tag="KR440_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBH0698221.1"
                     /translation="NILGGVKTCLKDEYMTMTYVSQGFLELTGYSCEDLNKIFKNRFK
                     DMIYKDDLDKFNCIILNQLEKQDKIEVEYRIVRKDGSIIWILERSKSINDNKGSECIQ
                     GVLTDITELKKIQEELDSKRFEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGY
                     TRKQLEDELNNKLIKLIFKDDRTELINSINKQLKLSDTFASEQRVEKRDGTIIWILLK
                     GRKIIYDNGKSVLCCVIVDITSAKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKM
                     NISPNWYNKFKYDPITENATKNIIKSVDIYHDDMDKFISLIKLTSQGSEYEECEIRLR
                     TRDTIYAWFSIKATTLFDNNGKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNK
                     KASQSLIEEYITLDKKRKGALMIIDIDNFKGINDNLGHLYGDAVLSEIANDLINLFRA
                     TDIVGRIGGDEFIVFIKDISETRDIINKAEELVKTFERSFLGKGRNYKISLSVGIAKF
                     PQDGMDYMTLFRNADMALYSAKGKGKNCYTLYDKKIDKVQYITNTRNAGDSDNVEMSF
                     KEHITNYIFDILYESSDIEVAVNLILSIVGKHFNVSRMYIFENSDDNRVCKNTFEWCN
                     EGVTSEINNLKNICYKDLGNYLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQC
                     KILEGNEFRGFIGFDECSYNRMWTNDEVNTLTFISKTLSMFLLKMRIQSKLEESLTIQ
                     KSVMDNMDIWAYVVDRNTYELLFINKKMLESASNTHVGDLCYKAIWNNQDKPCKKCPM
                     IYLKDGDDTYKSFMRNKKFEVQANITTTTLKWFDGKDACLIFGSEVTSIEAEENT"
     gene            2748..4682
                     /locus_tag="KR440_001164"
     CDS             2748..4682
                     /locus_tag="KR440_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBH0698222.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLENAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            4788..5783
                     /locus_tag="KR440_001165"
     CDS             4788..5783
                     /locus_tag="KR440_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0698223.1"
                     /translation="MKKYISIILLVVVTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSNIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            5755..6540
                     /locus_tag="KR440_001166"
     CDS             5755..6540
                     /locus_tag="KR440_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBH0698224.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            6528..7226
                     /locus_tag="KR440_001167"
     CDS             6528..7226
                     /locus_tag="KR440_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0698225.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            7349..7822
                     /gene="ybaK"
                     /locus_tag="KR440_001168"
     CDS             7349..7822
                     /gene="ybaK"
                     /locus_tag="KR440_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBH0698226.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKLYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            8019..8858
                     /locus_tag="KR440_001169"
     CDS             8019..8858
                     /locus_tag="KR440_001169"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HBH0698227.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            8871..9419
                     /locus_tag="KR440_001170"
     CDS             8871..9419
                     /locus_tag="KR440_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HBH0698228.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            9485..10666
                     /locus_tag="KR440_001171"
     CDS             9485..10666
                     /locus_tag="KR440_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HBH0698229.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEVKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYIYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            10839..11102
                     /locus_tag="KR440_001172"
     CDS             10839..11102
                     /locus_tag="KR440_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBH0698230.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            11628..12311
                     /locus_tag="KR440_001173"
     CDS             11628..12311
                     /locus_tag="KR440_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0698231.1"
                     /translation="MSSILLVEDDLSLIYGLEFSIQKSGFSIDIARTVKEAMQMYEEK
                     NYDLLLLDVSLPDGNGFQICEKVREVSNIPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNVASNVNELSSNNITIKLLENRVFKGEFEVELTTAEYKLLC
                     LFMKNKNIVLTRKNILDKLWDGNGSFIDDNTLSVYVRRLRNKIEDNPENPKFLLTVRR
                     MGYKWNVIK"
     gene            12322..13563
                     /locus_tag="KR440_001174"
     CDS             12322..13563
                     /locus_tag="KR440_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0698232.1"
                     /translation="MKIFSNKDIKIFFLILISILVMFIIVGQIVVISITNNYKKTLIE
                     HDYNIAGYLYSIGVDKSKIPSAFVAHEDKYILKGKEILDTAGYGFDIDVSFTPSVKLF
                     QSKYIKTMSIYLLVFFTMIIFILYMYFFRQQSKIEEASYKIDLFMNGNINERLNTYEE
                     GSLSKLFSSIDVMSTSLNTHILKEKQSREFLKNTISDISHQLKTPLTALIMYNQILQE
                     ESHNSDVVEAFVQKSQSELERIETLIKNLLKITKIDSKTIVLNKNNVNLKNLIVKILY
                     SFETRAEKEGKVISINGSENTLLFCDYEWMSEALSNLIKNALDNTKENDKITIEWFET
                     TITTIVSVKDTGNGIHVEDIHHIFKRFYRSKFSKDNQGLGLGLPLVKSIVEQHNGTIS
                     VESKLSQGSTFTISFLKLTNM"
     gene            13635..14318
                     /locus_tag="KR440_001175"
     CDS             13635..14318
                     /locus_tag="KR440_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0698233.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGRIFIDGKEICSMKEEKQTIFRRRNIGFIFQAYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKNHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSNDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGV
                     LTDLGVNRE"
     gene            14315..16645
                     /locus_tag="KR440_001176"
     CDS             14315..16645
                     /locus_tag="KR440_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBH0698234.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIIIAVCLVTAIFGLADMAI
                     QCQKIQFIKDDGNWHIVIRNIDEKFISEINSRVDIAHHGRGQVVDSGEFKSKEILIQG
                     LDEEVAKQFNLEIIRGRYPIGENETLIDEDAVKSFNLKIGDFIKIKLADGRNKNYKIV
                     GEYSSISSLRATDKHGLILSILGTQAIKKSETYDYYYITFKEKVNIRKATSDIKNTFN
                     LSDKQIGKNERLLSIIGQGDSDFSKPLYLTAGLLFVLVLVASIIMIYNSFNMSVIERV
                     KFFGLLRCLGSSKHQVKKFVVLESLILSIKAIPIGLVLGSLVTIISSIFLKYVNELFS
                     SMPILKVSFIGIAFGIIVGFLTVILSAIIPAKKASRVSPLSAVRGNLMSKEILNNGKT
                     SKVANTGSFKIETSMGLNHAFSRKKSFVLMTSSFAISIILFLGFSVLIDLMYQGLKPM
                     KPEAPHISIVDPNFKSVLTKEDINEISNIKGVKKVYSRMMTDIEATYNGKKTKSQLIS
                     YEKYQFNWAKKYLLKGEVNENKLQSENLALVEEGHGFDIGDKIYIKNRNAEKELEVGG
                     ILSSIPFEAKDKYIGNIITSENTFTNITGIKGYIIMDIQVDKDTPGDLIENIRRVVDD
                     NLEVRDKRQSNLEVKNSFYTMATFVYGFVFIIAIISMFNIINSMNISVTSRKNYYGVM
                     RAVGISNRQLRRMVIVEAGSYAMSGCLLGSILGLMLHRYMFISLVSSMFHLEWQVPFN
                     LLLITVLTMIIITLLSVRKPVKKICELDIIDSINAQ"
     gene            complement(16826..17668)
                     /locus_tag="KR440_001177"
     CDS             complement(16826..17668)
                     /locus_tag="KR440_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBH0698235.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKAGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            17963..18697
                     /locus_tag="KR440_001178"
     CDS             17963..18697
                     /locus_tag="KR440_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0698236.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            18707..19840
                     /gene="nagA"
                     /locus_tag="KR440_001179"
     CDS             18707..19840
                     /gene="nagA"
                     /locus_tag="KR440_001179"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBH0698237.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNITHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGDVVYKNI"
     gene            19939..20688
                     /gene="nagB"
                     /locus_tag="KR440_001180"
     CDS             19939..20688
                     /gene="nagB"
                     /locus_tag="KR440_001180"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBH0698238.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKLENTHLP
                     NGMADDIEKECLNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            20863..21135
                     /locus_tag="KR440_001181"
     CDS             20863..21135
                     /locus_tag="KR440_001181"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698239.1"
                     /translation="MLKIIMFSGILSSIIKLPIIGILPIGNIKKNTGISDSKKDGKQR
                     RSNLGISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
BASE COUNT         8594 a         2281 c         3800 g         6898 t
ORIGIN      
        1 taatatatta ggaggggtta aaacctgttt aaaagatgaa tatatgacta tgacatatgt
       61 aagtcagggg tttttagagc ttacaggtta ctcttgtgaa gatttaaata aaatatttaa
      121 aaatagattt aaagacatga tttataaaga tgatttagac aagtttaatt gtattattct
      181 aaatcaatta gaaaaacaag ataaaattga agttgaatat agaattgtaa gaaaagatgg
      241 aagtataatt tggattttgg agagaagtaa gtctataaat gataataaag gaagtgagtg
      301 tattcaggga gttttaactg atataacaga acttaagaaa atacaagaag agttagattc
      361 aaaaagattt gaaatacagg ctatcaatga tagtattgtt ggtggaatag tgataactga
      421 gataaatgat gattttgata gtatatattt aaatgaaggt tatttaaata tgattggata
      481 tacacgcaaa caactggaag atgagctaaa taataaatta atcaaactta tttttaaaga
      541 tgatagaaca gaattaatta atagtataaa caaacaactc aaattaagtg atacatttgc
      601 atctgaacaa agagttgaaa aaagggatgg tactattata tggattttat taaaaggaag
      661 aaagataatt tatgataatg gaaaatctgt actttgttgt gttattgtag atatcacttc
      721 tgctaaatta ttagagcaag aacttaaaaa gagtgaagaa cgtcatgaaa tcattctaaa
      781 gcagacgcaa aatatcataa tagaatggga tatagaaaaa gataaaatga atatatcacc
      841 taattggtat aataagttta aatatgaccc tataactgaa aacgcaacaa aaaatataat
      901 taaatcagta gatatatatc atgatgatat ggataagttt atttcattaa ttaaacttac
      961 ttcacaaggt agtgagtatg aagagtgtga aattagactt agaactagag atactattta
     1021 cgcttggttt agtataaagg caacaacttt atttgataat aatggtaaac cttataaagt
     1081 agtaggaagt ttgactgata ttgatgaatc aaaacgagaa gctgaaaagt tgaaagaaag
     1141 ggcagaaaaa gaccctctta cagatttgta taataaaaaa gcatcacaat cacttataga
     1201 agagtacata acattggata aaaaaagaaa aggtgcttta atgattattg atattgataa
     1261 ctttaaagga ataaatgata atttaggaca tttatatggg gatgctgtat taagtgaaat
     1321 tgcaaatgac ctaataaatc tatttagagc aaccgatata gtaggcagaa ttggtggaga
     1381 tgagtttatt gtatttataa aagatatttc tgaaacaaga gatattataa ataaagcaga
     1441 agaattggta aaaacatttg aaaggtcttt tttaggtaaa ggacgaaatt ataaaatatc
     1501 attaagtgta ggaattgcta agtttccaca agatggtatg gactatatga cactatttag
     1561 aaatgcggat atggctcttt actcagccaa agggaagggt aagaattgtt atacattgta
     1621 tgataaaaaa attgataaag tccaatatat aactaataca agaaatgcag gagatagtga
     1681 taatgtagag atgtctttta aagaacatat cacaaattat atatttgata tactatatga
     1741 atcaagtgat atagaagtgg cagtaaattt aattctaagt attgtaggta aacactttaa
     1801 tgtgagtcgc atgtatatat ttgaaaattc agatgataat cgagtatgta aaaatacatt
     1861 tgaatggtgt aacgaaggtg taacctcgga aattaataat cttaaaaata tttgttacaa
     1921 ggatttaggc aactatcttt taaattttaa tgcagatggg ttattttatt gtaatgatat
     1981 tgaaaattta ggtagtgatg tgaaaaaagt acttaagcca caaggcatac gttcaatatt
     2041 acaatgtaaa attttagagg gcaatgaatt tagagggttc ataggatttg atgaatgttc
     2101 atataatcgt atgtggacta atgatgaagt gaacactttg acatttatat ctaaaacgct
     2161 cagtatgttt ttattaaaaa tgcgtattca gagtaagcta gaagagtctt taacaattca
     2221 aaaatctgtg atggataata tggatatatg ggcatatgtt gttgacagga atacctatga
     2281 attattattt atcaataaaa aaatgcttga gtcagcatca aacactcatg taggtgattt
     2341 atgttataaa gcgatatgga ataatcaaga taaaccttgt aaaaaatgcc ctatgatata
     2401 tttaaaagat ggtgatgata cttataaatc atttatgcgc aataaaaagt ttgaggtaca
     2461 ggcaaatata actacaacta ctttgaaatg gtttgatggg aaagatgcat gtttaatatt
     2521 tggaagtgaa gtaactagta ttgaagctga agaaaatact taaaatactg tgttatatat
     2581 atacttaaaa tcgcaatttg aatttattag acttaaaatg tctatataaa tcatttaagt
     2641 tgtggtttta agtatttttt atgatataat taagcgttaa gaacaaaatt aaaagatact
     2701 tttgtataaa aaaatatttg ttaaatatat attgagcagg tgataatatg gaaaataagt
     2761 ttttaccaat aagcaaacaa gatatgatag atagaggatg ggaagaatta gattttgttc
     2821 tagttacagg agatgcttat gtggaccatc atagttttgg aacagcaata atatctagag
     2881 ttttagaaaa tgctgggtat aaagttggaa taatagctca accagactgg agaactacag
     2941 atgattttat gaagctagga aaaccgagac ttgcatttct agttaatgca ggaaatatgg
     3001 attcaatggt caatcattat tctgtaagta aaaagcatag agaaaaggat atgtattctc
     3061 caggtgggaa aatgggttat agaccagata gagctactat agtatattgc aataaaataa
     3121 gagaagcata cagtgatgtt gcaatagtaa taggtggaat tgaagcgagt ttaagaagat
     3181 ttgcacacta tgattattgg agtgataagg ttagaaagtc tatgttaata gatagtggag
     3241 cagatttatt agtttatggt atgagtgaaa agcagattgt agaagtagcc aatgcactta
     3301 atgatggata tgacccaaaa tatattagac atattgatgg gacatgctat atttcagata
     3361 cattggaaga aatatatgat aaatatatat taattccatc atataaagaa atttgtgaag
     3421 ataaaatgaa gtatgtagaa gcttttaaaa ttcaatatga tgagcaagac ccatttagag
     3481 gtaatataat tgttcaacct catggaagta agtatctggt tcaaaataag ccagaaaaac
     3541 cacttagtag agaagaacta gatgaagtat atggattacc atatcagaag acataccatc
     3601 ctgtgtatga aaaatttgga ggaataccag ctatagaaga agtaaaattt agtatagtaa
     3661 gttcaagagg ctgttttggt agttgttcat tttgtgcaat aacatttcat caaggtagag
     3721 cagtgcaaag tagaagtgaa aaatcaatta ttgatgaagc aataggaata acaaatttag
     3781 atgattttaa agggtatata catgatgttg gaggtccaac agctaatttt agaagaccag
     3841 catgtaaaaa acaaattact aaaggtgctt gtaaaaatag acaatgctta tctccatcac
     3901 catgtaaaaa tttagatgca gaccacagtg aatacttaca tttacttaga gctgtaagaa
     3961 aattacctaa aataaaaaag gtgtttgtgc gttctggaat tagatacgat tatgtaatgg
     4021 cagataagaa caataaattt cttagagagt tgatagagca tcatgtaagt ggtcagttaa
     4081 aggtagcccc agaacatata tcagaagaag ttttaaagta tatgcaaaaa ccagcaggaa
     4141 aaacttatga taaatttaga cagaagtttt ttgctataaa tgaagaatta ggtaaaaagc
     4201 aatatttgat accctattta atgtctagtc accctggttc taccttaaat agcgctatag
     4261 agttagcaga gtatttaaga gatactcact accaaccaga gcaggttcag gatttttatc
     4321 caactccagg gactctatct acaaccatgt tttatacagg tattgacccg cttacaatga
     4381 agccagttta tgttcctaaa tcaaaacgtg acaaggcaat gcaaagggct ttattacaat
     4441 atagagcacc tagaaattat gatttggtat actctgcgtt agttgaagca ggaagagaag
     4501 acttaattgg ttttggacat agatgtctta taaagccaaa aaatgaaaag ccttatttta
     4561 atagaaataa ttctaaaaaa aatgtatcta aaggaacaaa taaaaacaaa aaaactaata
     4621 caaataacag aagtaataaa aatcaacaaa aaagttcaac taaaaaaaag aaaaaacatt
     4681 aaagtataaa taaaaatact taaaaaagaa ggattattcg ataaattaac agaaattagt
     4741 ataaataaat ataattattt taatgacgtt acctttggga ggaagagatg aaaaagtata
     4801 tttctataat attattagtg gtggtgacta tggttgtttt ggtaggatgt tcaccaggaa
     4861 aagataaccc taaaaataag gagctatcag ttgtcaaaca agtaaaagta gctgttccag
     4921 atggactacc agcagtggca atagctaaac ttgcaaatga aaatccagag attaaagaag
     4981 gatatgaaac agtgtatagc attgaaaaga cacctgaggc gatttccact agagtgatga
     5041 aaaaagatgc agatatagct atagttcctt caaacatggc tgcaatagcg tataataaaa
     5101 cttcatcata taatattgtt ggaactgtag gaatgggttc actttatttg gtgtcaacag
     5161 aaaatataaa agattattct gatttagttg gtaaagaagt tggatgtact ggaaaaggac
     5221 ttactccaga cataactata aaaagtcttt tgttacaaaa ggatataaat tattcaaata
     5281 taaaatttaa ttatgtaaat tcagcaagtg aactggtacc attattggct actggtaaag
     5341 ttaagacagg tattgttcca gaacctgcac ttagtgcttt gatgtctaaa aatccagaca
     5401 taaaaattat aaaaagttta aatgactcat ggaaggaagt aagtggttca aaagacggtt
     5461 atcctcaatc tactttaatt gtaaagtctg aatttttaag agataataaa gattttgttg
     5521 attcttttgt aggtcaatta tcaaatagta tagattgggc aaacaaaaat cctgaagaat
     5581 taggtgcata ttctgaaaaa ataaaaatat ctactgaatc aaagataata ggaaaatctc
     5641 ttgaaagagc taatttaaaa tatattccag ttaaggacat gattaaagat tataaaaatt
     5701 actatgagaa attagctaat tttgatgata agacattagg aggcaaagta ccagatgaag
     5761 caatatactt tgttgagaaa taaaaaaaat aaagaacaat ttatagataa aatggaggta
     5821 acactttctt gtgttatcct cttattttta tggcaaatta ttgctttaaa aattgataat
     5881 gatatttttt taccaacagt aggtcaagta tttaatagta taaaagagat tatgctaaat
     5941 tctagtttct atatagatat actttactct atggggagat gtttttttag ttttttactg
     6001 gctatgattt ttgccattat actttcaata gtatcttatt taaatagatt ttttagaaac
     6061 ttattaaagc caatcaatgc tttgacacgt tcaataccaa ctatgatatt ggtagtactt
     6121 gcacttattt ggtttgaaaa ggatagtacc ccttttatag ttggatttac tatagttttt
     6181 cctatattgt atgataacgt tttgggcgct atattgaata tagacaagaa cttattagaa
     6241 atggctaatg tgtataaaat aaggtttata gataaggtgt taaaaattta tttaccagcc
     6301 ataaaatttc aaattatatc tatactagta tcaacattct ctttaagttt gaaagttgct
     6361 atagcaggag aggtatatag tcaacctaca tatggtattg gaactatgat acagactgaa
     6421 aaaattaact ttaatacatc tggaatattt gcatggataa ttattgttct tttaatatct
     6481 gctattttac aaatagccca aaagttttta gcaaggagag cttttttatg gaaaagataa
     6541 agttgagtat tgaaaatata aataaaagat atgatagtcg tattatattt agagatttta
     6601 atattgattt ttatgtaaat gaggtaaatt gtatccttgg aaaatctggt tgtggaaaaa
     6661 ctacactctt gaacataata agtgggatta ttaaaaatga caccaacaat ctaaatatca
     6721 aagaaaattt aaatagagta ggaaataagc ttgaagctag ttacatattt caagatgata
     6781 gacttataga ctggttaaca gtagaagaaa atataaagat agttgtaaat aaatactata
     6841 ataaaactca attaaataaa atttgtgatg aatacttgga attggttgga attagtgatt
     6901 ataaaaaatt ctatcctcag atgcttagtg gaggtattag acaaagagta aatattgcta
     6961 gagcttttat atatccatca aaaaacatta taatggatga accttttaaa tctattgatg
     7021 caaagaatac acagcttata atggataatt tcagaaatat attaagaaaa gaaaagagaa
     7081 ctgtattatt tgtaactcat aatattgagg aagcattgtt tttagcagat aggatattta
     7141 ttttaggaga ctctccaatt agaataaaaa aaatattaaa aaattctaag gaattggaaa
     7201 aaaatgaagt tttgaagttg atatagagtt ataataatac tgtcgtttta aaatatattt
     7261 ggttaaaatc tataagacta tattaaaata gtacagaata ttcaaaactg tgtataatgt
     7321 tcgtatttta taaaaaggaa gtgaatgtat ggttaagact aatgctatga gaatattaga
     7381 ttcaaataag attgattata aagtaatgtc ttatgaagtg aaaagtgaac atgtggatgg
     7441 tgttgaggtg gcacatgata taggaagaga tgtaaatgaa gtgtataaga cattagtaac
     7501 tcaaggagtt agtaagaata tatatgtata tgtaatacca gtacatgaaa acttagactt
     7561 aaaaaaggct gcaaaagtag ctaaagagaa aagtgtagag atgattcatg taaaagatat
     7621 aaataagctt acaggttata ttagaggcgg atgttctcct ataggtatga aaaagcttta
     7681 taaaactttt gtaaatgaaa gtgcaaagaa tttagataca attattgtga gtgcaggaaa
     7741 aataggatat caaatagaac tatctccatt tgatttacaa agacttataa aagtagaatt
     7801 tgtagatgta ataaaaaaat aagaattaca tataaaatat agagcttata ttcttaataa
     7861 taggtgttgt aatattaata aaatgaataa taattttaaa ataaatattc agttacttat
     7921 gtgtaattgg tataaaaata ttgagaaatt ttaaaaacaa tggtaaagtg taagtataat
     7981 cacgaatatt ttttataaag taatggaggg atgttcgctt gagaaaaata aaatctgaac
     8041 aaatcgtcga gcaggtaaaa aaactttgta tagaagcgag cctatattta ggagaagatg
     8101 ttttaagttg tataaaagaa aaagcgaaaa gcgaaaagag tgaagttgga aaaaatatat
     8161 tgaatatatt agttgaaaat gctgaaatag ctaaagaaaa aaatatacca atatgccaag
     8221 atactggtat ggctgtattt tttgttgaaa taggacaaga agttttaatt gaaggagaca
     8281 cacttactga tgctataaat gagggagtaa gacaagggta tgaagaaggc tacttaagaa
     8341 aatctgttgt aagtccaatt aacagagtaa atacaaaaga taatacacct gctgttattc
     8401 attatgatat ggtaaaaggt gataaaatta aaatagaatt tgcagctaag ggatttggaa
     8461 gcgaaaatat gagtaagatg aaaatgttaa agccatcaga tggattggaa ggaattaaaa
     8521 aattcataat agatacagtt tcagaagctg gacctaaccc atgccctcct atggtaattg
     8581 gagttggtat aggtgggact gtggacaaat gtgctcaaat agctaaaaag gcacttttta
     8641 gagaactagg tgaatttaat aaggacgaaa atatagcaaa acttgaaagt gaactattaa
     8701 cagctattaa taaacttggt ataggacctc aaggcttagg tggtactaca acagctttgg
     8761 gattaaacat agagactttt cctacacaca tagcaggtct acctgttgtt gtaaatataa
     8821 actgccatgc atcaagacat aagaaggtag taatttagga ggaaaataaa atgataaaaa
     8881 taactacacc tgtaaatgaa attgatatag caaaattaaa ttgtggagat acaattagtt
     8941 tgagtggtat actttataca gcaagagatg ctgctcataa aagattgata gattgtataa
     9001 ataaaggtga agaattacca tttgacgtat atggtcaagg aatatattat gtaggaccta
     9061 ctcctacaaa gccaggagaa gttataggtg ctgcaggtcc aacgacgagt tatagaatgg
     9121 atgatttgac tatacctctt ttagaaagag gtcttagatt gatgataggc aagggaaaaa
     9181 gaagtgatga agttatagaa ggtatgcaaa aatacggagc agtttatctt gctgcaatag
     9241 gaggagctgg agcgtatatt tcaaattcaa taaaatcatg tgaaattatt gcatatgaag
     9301 atttaggagc tgaagctata agaaagattg tagtagaaga ccttaaactt acagtagcaa
     9361 tagatagcta tggaaataat atatatgagc aaggtcgagc aatttatgaa tgtaaataat
     9421 gtttagaaat aaatatgaat atcaaaatta aaaatagttg tgtatatttt aaggaggaaa
     9481 attgatgtct aaggattatg caaaattggc attagaaatg catgaaatca acaaagggaa
     9541 agtaagtgta gaaagcaagg tagaagttaa aactaaggat gatttatcaa cagcatatac
     9601 accaggggtt gctgaacctt gtttaaaaat acatgaaaat caagatgatg tttatatata
     9661 cacttctaaa gggaatctag tagcagtagt tagtgatgga agtgctgtac ttggattagg
     9721 taacattggt gctgaagcat ctatacctgt tatggaaggt aaagctatat tatttaaaca
     9781 atttgcagat gtagacgctt ttccaatatg tttaaagact aatgatgtag atgagatagt
     9841 taaatctgta gagttgatgg agcctgtatt tggaggtata aatttggaag acatatcttc
     9901 tccaagatgt tttgagatag aagagagatt aaaaaaatct ttaagtatac ctgtttttca
     9961 tgatgaccaa catgggacag caataatagt tgcagcagcc ataataaact caataaagtt
    10021 aattgaaaat aaaaaaatag aagatttaga aatagttata aacggagcag gagctgctgg
    10081 tatagccata gcaaagatac tacttaatat gaatgttaaa aatataatct tatgtgatag
    10141 aagtggagca ttggaagcta gtatagaaaa tctaaactat gttcaaaaag aaatgttaaa
    10201 agtaacaaat ataagaaatg aaaaaggacc tttaaaagat attataaaag gaaaagatgt
    10261 atttattggt gtttcaggtc caggagctgt tacaaaggaa atggttgaaa gtatgtctga
    10321 gaagccaata atattggcaa tggcaaatcc tactccagag ataatgccag aagaagcaaa
    10381 attaggtgga gcatttataa tgggaacagg tcgttcagac ttccctaatc aagtcaataa
    10441 tgtactggct tttcctggaa tatttagagg agcattagat gttaaggcta aagagataaa
    10501 tgaagaaatg aagatagctg ctgcatatgc aattgctaat acaataagtg atgaagaaat
    10561 atgcccagag tatatattac cagatgtatt taataagaat gtagtaaaaa atgttgctaa
    10621 ggcagttaaa gaagcagcga taaaaactaa agtaaataga atataaattt ttataactta
    10681 ttaaaaacta gaaaagtata aaagactagt gaaattatgt acagtgggat gattttatta
    10741 gtctttttat tttaaatatg acacaataat gtcatattaa gcttgatata attttaatat
    10801 ggttaggagc caaagaaaaa tataaggagt gatttaaaat ggaagaaact aaattttgcc
    10861 aatgctgtgc aatgccaatg ggacaaactg atgaattata tgggacaaat aaagatggaa
    10921 gtaaaagtac tgattattgt agttattgtt ttaaagatgg tgagtttaca gctgatataa
    10981 gtatggaaga gatgatagaa gtttgtatcc ctcatatgct tcaaagtaat aaggatatga
    11041 ctgaagatga agcaagaaca atgatgaaca atttctttcc aacattaaaa cgctggaaat
    11101 aattttatgc aaaattagtt atatgcaaaa catgaaatat ggtgttgaga ataaggggtg
    11161 gtgtgcttta tagcatacca cctgttttgt tacttgctag ttcagttatt cttgtatgct
    11221 taaatatgtt ctcatctctc tttctatttt aaattgtaaa aatctaaaat atgttattta
    11281 tattctactt ctttatccat gtctttttct acctcaagtc aaatacaact acaaaatcat
    11341 cattttcaat tcactgttta atagaattaa cagataaaga gttataaaat tttctatttt
    11401 ttatataaaa taatacaggt caagctgact tctagctact tgggatagtg tatatattta
    11461 tgatagttat aggagtaata ccaatcatta gactgttgat ttgtgtagtc ttttgtaaaa
    11521 acatttgttt taatatattg agtttttgaa aaagtaaaat tataattata ataaattaag
    11581 tgtaataatg tattagaagt aaaaataatt aaaatagagg tgattaaatg agcagtatat
    11641 tgttagttga agatgattta agcttgatat atggattaga gttctctata caaaaaagtg
    11701 gatttagtat agacatagca aggacagtaa aagaagctat gcaaatgtat gaggaaaaaa
    11761 attatgactt attgctttta gatgtatccc tacctgatgg taatggattt cagatttgcg
    11821 aaaaagtacg agaggtttct aatataccca ttatattttt gactgcttca gatgaagagg
    11881 taaatgtagt gatgggtctt gatatgggtg gcgacgatta tataacaaaa ccttttaaat
    11941 taaatgaatt aatttcaaga ataaaagcat tacttagacg ttataatgtt gcaagtaatg
    12001 tgaatgaatt aagttcaaat aatattacta ttaaattact tgaaaatcgt gtttttaaag
    12061 gtgaatttga ggtggaactt actacagcag aatataaatt attgtgctta tttatgaaaa
    12121 ataaaaatat tgtacttact agaaaaaaca tattggacaa gttatgggat ggaaatggta
    12181 gctttataga tgacaataca ttatcagtat atgtaagacg attgagaaat aaaattgaag
    12241 ataatcctga aaatcctaag tttctattga ccgttagaag aatgggttat aagtggaatg
    12301 taattaagtg agggtattaa tatgaagata ttttctaata aagatataaa aatatttttt
    12361 ttaattctta tatctatttt agtaatgttt attatagtag ggcaaatagt ggttataagt
    12421 ataactaaca attataaaaa aacgttgata gaacatgatt ataatatagc tggatactta
    12481 tatagtatag gtgttgataa atctaaaata ccatcagcat ttgttgcaca tgaagataaa
    12541 tatattttaa aaggaaaaga aatactagat acagctggat atggatttga tatagatgtt
    12601 agctttacac caagtgtaaa attgtttcaa agtaaatata taaaaactat gtctatctat
    12661 ttattagtat tttttactat gattattttc atactatata tgtatttttt taggcaacaa
    12721 agtaagatag aagaggctag ttataaaatt gatttattta tgaatggaaa tataaatgaa
    12781 agactaaata catatgaaga aggtagctta tcaaaattat ttagttcaat agatgttatg
    12841 tcgacatctt taaatacaca tatattaaaa gaaaaacaga gcagagaatt tcttaaaaat
    12901 acaatttcgg atatatcgca tcaattaaaa actccactta cagctttaat aatgtacaat
    12961 cagatactac aagaagaaag tcataatagt gatgtcgttg aagcttttgt acaaaaaagt
    13021 caaagtgaac tagaacgcat tgaaacattg attaagaatt tactaaagat tacaaagata
    13081 gattcaaaaa caatagttct caataaaaat aatgttaatc ttaaaaattt aattgtcaaa
    13141 attttgtata gctttgaaac tagggcagaa aaagaaggaa aggttatttc tataaatggt
    13201 tcagaaaata cattgttgtt ctgcgattat gaatggatgt cagaggcttt aagtaatctt
    13261 ataaagaatg cattagataa tacaaaagag aatgataaaa taacgataga gtggtttgag
    13321 acgactataa ccaccattgt ttcagttaaa gacactggaa atggaatcca tgttgaggat
    13381 attcatcata tatttaaaag gttttataga agtaagtttt caaaagataa tcaaggttta
    13441 ggactgggat taccactagt taaatctatt gtggaacaac ataatggtac tatttctgta
    13501 gaaagtaaac tatcacaagg aagtacattc accataagtt ttttaaagct tactaatatg
    13561 taagataaaa tacatcttgc agtaaggtat atagattatt attatattaa tcaattagaa
    13621 aggtgagaga gaaaatggat attcttgtgg ttaataattt atgtaaaaca tatggaaatg
    13681 gatatacaaa agtagaggca ttgaaagatg tatcctttac agtttcaaaa ggagagtttg
    13741 tgactgtagt tggagaatct ggttcaggaa agagtacact attaaatctc ttaggaggtc
    13801 ttgatactcc aacatcaggg agaatattta ttgatggtaa agaaatatgt tcaatgaaag
    13861 aggaaaaaca gactattttt agacgtcgta acatagggtt tatatttcag gcatataacc
    13921 ttataccaga actaactgtg gagcaaaata taatattccc attgttatta gattataaaa
    13981 aaccagatac tatttactta gatgaagtat tgactatttt aggtcttaaa gataggaaaa
    14041 atcatcttcc aaatcaatta tctggtggtc aacaacagag agttgcaatt ggaagggctt
    14101 tgattacaaa gccaagtatt atactggcag atgagccaac gggaaatctt gacagtaaaa
    14161 atagtaatga tgtaatttca cttttaaaag tgactgcaga aaagtataat caaaccatta
    14221 ttatgataac tcataatacg aatatagctt ctacagcaga tagaatacta caggtttctg
    14281 atggtgtgct tacagacctg ggggtgaata gagaatgaga agctacttag aattagtacc
    14341 acaatatgca aaagtacata agaaaaaaaa tagaactaca attttatgta ttattattgc
    14401 agtatgctta gttactgcta tctttggact tgcagatatg gctatacaat gtcaaaagat
    14461 acaatttatt aaagatgatg gaaattggca catagtaatt agaaatattg atgaaaagtt
    14521 tatatcagaa ataaatagta gagtagatat agctcaccat ggtagggggc aagtcgttga
    14581 cagtggagag tttaaatcaa aggagatttt gatacaaggt ttagatgaag aagtagcaaa
    14641 acaatttaac ttagaaataa taaggggtcg ttatcctatt ggggagaatg agactttaat
    14701 agatgaggat gcagttaaaa gctttaatct aaaaatagga gattttatca aaataaaatt
    14761 agcagatggt agaaataaaa attataagat agtaggtgaa tattcatcaa tatcaagctt
    14821 aagagctaca gataaacatg gattaatact ttcaatattg ggtacacaag ctattaaaaa
    14881 atcagaaacg tacgattatt attatattac atttaaagaa aaggttaata ttagaaaagc
    14941 cacttctgat attaaaaata ccttcaattt aagtgataaa caaatcggta aaaatgaaag
    15001 attactttct attataggtc aaggcgactc tgacttttct aaaccactgt atttaacagc
    15061 aggattactt tttgtacttg ttttagtagc aagtattatc atgatatata atagttttaa
    15121 catgagtgta attgagagag ttaaattttt tggtctgtta cgttgtctag ggtcatcaaa
    15181 acatcaagtt aaaaaatttg ttgttttaga aagtttaatt ttaagtataa aagctattcc
    15241 aataggctta gtattaggaa gtcttgtaac tattatatct tctatatttc ttaagtatgt
    15301 taatgagtta ttcagctcaa tgccaatttt aaaagttagt tttattggta ttgcatttgg
    15361 aataattgta gggtttttaa ctgtaatatt atcagctatt attccagcaa aaaaagcttc
    15421 ccgtgtatct ccacttagcg ctgtacgtgg gaatctaatg tccaaagaaa ttttaaataa
    15481 cgggaagaca agtaaagttg caaatacagg aagttttaaa attgagactt ctatgggatt
    15541 aaatcatgct ttttcaagaa agaaatcttt tgtacttatg acttcttcat ttgccattag
    15601 tattatacta tttttaggtt ttagtgtgct tatagacctt atgtatcaag gacttaagcc
    15661 tatgaagcca gaagctcctc atatatctat tgttgaccct aattttaagt ctgttttaac
    15721 taaggaagat attaatgaaa taagcaatat caaaggagta aagaaagtat acagtagaat
    15781 gatgacggat atagaagcta catataatgg gaagaagaca aaatcacaat taatttctta
    15841 tgaaaagtat caatttaatt gggcaaaaaa atatctatta aaaggagaag ttaatgaaaa
    15901 taaactacag tcagaaaacc ttgcattagt agaagagggg catggatttg atataggaga
    15961 taagatatat attaaaaata gaaatgctga aaaagagtta gaggtaggag gtatactttc
    16021 ctcaattcca tttgaggcta aagataaata tattggaaat attataacat ctgaaaatac
    16081 ttttacgaat ataacaggta taaagggtta tataataatg gatattcagg ttgataaaga
    16141 tactcctgga gatttaattg aaaatataag acgtgtagta gatgataact tagaggtcag
    16201 agataaaaga caaagtaact tagaggttaa aaattcgttt tatactatgg caacatttgt
    16261 atatggattt gtgtttatta ttgcgattat aagcatgttt aatatcataa atagcatgaa
    16321 tataagtgtt acaagtagaa agaattacta tggtgttatg cgtgcagtag gcatatccaa
    16381 cagacaatta agaagaatgg taatagtaga ggcaggttca tatgcaatga gtggttgttt
    16441 attaggtagt atactaggat taatgttaca taggtatatg tttatatcgt tagtaagttc
    16501 aatgtttcat ttagagtggc aagtaccttt taatttacta cttatcactg tactaactat
    16561 gataataata actttattat cagtaagaaa accagtcaaa aagatttgtg aattagatat
    16621 aatagactca ataaatgcac aataaatata gctgttgatt taaattaaaa aaacgaagta
    16681 aaaatgaaat agaaaatatt tttcattttt acttcgtttt ttatattata aaatttttgt
    16741 tatgttacaa aatttctata aagatgatgt gaatctaaac attatatgac aacgtgagca
    16801 tttaagttaa aatattttaa atatactatt ttagataagt tccttcttca tcttgataaa
    16861 ttaatccttt tttcataagc actccaagag ctcgtttaaa atttttctta cttgaattaa
    16921 ataatataga aatatcttta gggtcagatt tatcattaaa tctcatatat ccatcagaac
    16981 cctctaaata acttaaaatc ttattttcta aagtatcaag ttcatccttt ctatttcctc
    17041 taggagaaag accaagtttt ccatcttcat atatttttat gacatgtaaa ttttcaagaa
    17101 catcaccagc ttttagttca gtaaagtact cattatgaag tattacacca gcatatttat
    17161 tgtctacaca tatcattgca gaattattag tttgaaatcc atatacaact ccagttacaa
    17221 tatctcctac attgtaagta tggtctgtta ataaatatga atctatatct gtagtagcag
    17281 caattctttt gcttttgtct aaatatatat aaaataaata tttttcgtct ctaaatagtg
    17341 gataagtctt agctttaaaa ggaactagta tgtctttagg aagtcccata tctataaaag
    17401 taccaatgtc cgtattatca actactttta aatgtgctac atccccaacc ttagctaaag
    17461 gcggtattaa agttgcagca gttctgtcat cagaatcttt aaatatgaaa gcatttactt
    17521 catcacctat gtttatttca ttttttccga ttaatctatt atgaagaagt atatcgtctt
    17581 ttgtgttatt agtttgtcca tccaaaaaat atccaaattc agtttttctt tttactgtaa
    17641 gtttattaaa atcacctatg tttatcaaat taaaatcatc ctctcatttt atatttataa
    17701 tttagccata atatacttat gtagtagttt gtcacagtat ctatattagt attagtgaaa
    17761 tttacaataa ataatatagt tatttatgta tttttcaatt ctctagcttc ttatatagta
    17821 taaactccaa tttctttttt gtctttaggt tttactaaaa tttttacaaa ataagttaaa
    17881 aagtattgaa ataaaatttc ttttaaagta taattggtat ataccaaaat tggtatatac
    17941 catatcgaaa agtggtgact atatgaaaat agtaaataaa tctagtaata taccattgca
    18001 tactcaatta agttcaataa taagagagat gatagaaact ggtgagctta aagagggtga
    18061 tgcaataatg ccagaaagag aactttgcaa catacaaaat gtaagtcgta tgacagtaaa
    18121 taaaaccatt gttggattag tcacagaagg acttctttat agggtacaag gtaaagggac
    18181 attcgttgca aagcaaaaaa agaagtatca gttttcaaat gtaaaaggtt ttactgatgt
    18241 tatgaaagaa aagggagtaa acataaaaac ggatatatta tcctttgaga tggaactacc
    18301 agatgattta gttaaaagaa agcttggaat aagtgataat actacaaata tatataagat
    18361 tgtcagactt agatatactg atggagagcc atttggatta gaaatagtat atctatcaga
    18421 agaaatgtgt aaaggtctaa caaaagggat attggataat tcttcattat atagggtttt
    18481 aaatgaaaaa tatggctata agatacaaaa agctgaacaa gtaatggagc cagtaatact
    18541 ttcagatgaa gaaagtaagc ttcttgaaac agatgaaggt gctttagctc ttaaacttca
    18601 tagaaattcc tataatagag aaggtagccc tatagaatat accatatcaa tatttagaac
    18661 agataaatat caatatgaaa tagtattgag tgagtaggtg aataatatga aatgtttaat
    18721 taatggaaag ataatattaa aaaatcaaat cttggaaaat aaagtattag tatttgatga
    18781 aaaaataatt gatatagctg atagtgtacc aaaagattgt gaagttatag atgctgatgg
    18841 aaaatatata tctccagggc ttattgatat tcatatacat ggaaatatgg gaaaagatac
    18901 aatggattcg actgatgaat ctattgaaac aatttcaaaa tcaattatga gacatggagt
    18961 aacatctttt cttccaacta caatgactat ggataaagaa catgtttatg atgcacttga
    19021 agttattaaa aaagcccaaa atagaaagct tgaaggggca caagtattag gagcacattt
    19081 agaaggacct tttataaatg agaattataa gggagcacaa aatgaaaaat ttataataaa
    19141 ttcaaaatac gaatttataa aagaatataa agatgttata aaagttataa cttatgctcc
    19201 agaaaaagat attgattttg attttactag agaaataaaa agatgtacag atatagtttt
    19261 atctattggt catagtaatg caaattatga ccaagctaaa gaagctataa atttaggagt
    19321 aactaatata actcacatgt tcaatgcaat gactggttta aaccatagag acccaggcgt
    19381 tgttggtgca gcacttacaa ctaatgtgta cagtgagcta atagcagata caatccatat
    19441 aaataaagat ttatttcaat ttatactaaa taataaaggg aaagaaagac tgattttaat
    19501 aacagattct atagaagctg gtggtcttga agatggcaat tattctcttg gaggtcagaa
    19561 agtcatagtt aagggtaatg aagctagact tgaaaatgga gcattagctg gaagtgtgtt
    19621 gtctttaaat aaaatggtat ttaacttctt agataataca aaccttaaag tgaatgaagc
    19681 aattaattta gcttctttaa atccagcaac atctttggga ataaatgaca aaaaaggaag
    19741 cttagaaata ggtaaggatg cagatatagc tgtatttgat gagaatttag attgtaaaat
    19801 gacactttgc ttgggggacg tagtatataa aaatatatag tatacaaaac aaactttaaa
    19861 gacagtaaaa acaatataaa aaataataaa ttaaacttac attagttaaa gtagtagtta
    19921 aaatttagga ggaataagat gagagtatta gtgtgtaaag attatgatgg aatgagtaaa
    19981 aaagccgctg aaatgatagc agctcaaata gttttaaaac caaattcaat actgggacta
    20041 gctacaggaa gtacaccagt aggtatgtat agagatttag taaaaaaata caatgataat
    20101 atagtagatt tttcagatgt gatgagcttt aatttagatg aatattataa gttaccaata
    20161 tctaatgacc aaagctatga ttattttatg aaagagaatc tatttaatca tgtaaacata
    20221 aagctagaaa atactcatct accaaatgga atggctgatg atattgaaaa agagtgtctg
    20281 aactatgaag cttctataga tgcagctggt ggtatagatg tacaagtact tggaataggt
    20341 cgtaatgctc atataggttt taatgaacct gatactaaat ttgcaaaaag aactcatgta
    20401 gttgaactta cagagtctac aatagaagct aatgcaagat tctttaaatc aagagaagat
    20461 gttcctaaaa aagctgtaag tatgggaata ggttcaatat tgaagtctaa gaaaatattg
    20521 cttttagctt caggagaaga aaaagctgat gcagtatata atactgttta cggagatata
    20581 actccagaag ttcctggttc tatacttcaa cttcataaag acactatagt tatagtagat
    20641 gaagcagcag caagtaaact gaatccaaaa gattataaat tagtataaga atagaatgta
    20701 gtttaagtga aaaatagcta tcattgaata atcaaattta tttaatgata gctattttta
    20761 tttaaatttt ttacttcttt ttatttaaat tagttattat tgtttaaatt acttgcttat
    20821 tactttttac gaaaactcgt tatcaagaag tatccgaata agatgttaaa aattattatg
    20881 tttagtggga ttctatcaag cataataaaa ttacctataa ttgggatatt accaattgga
    20941 aacataaaga agaatacagg catcagtgat agtaaaaaag atggaaaaca aagaagaagt
    21001 aatcttggaa tatcaatata ccaactacca tcctttttgt attctctaat caataattca
    21061 gttcctagta acaaaccaat accaccagag gaaattaaat taataaaaat gtatctttta
    21121 aagtcttgca tataaccagt gtgaaattga gtatagaaat cataatttaa tttaacggct
    21181 aaaaaatata taagcaatag aatgattaaa aataaaagat atctaagagc ttttttcatt
    21241 ttaaattctc ccttcagaaa tactttattc agttttattt tgttggaatc tgattttatc
    21301 atttagttta agaaatccat aatattagtg aagaatgaaa taagtattat atatgaataa
    21361 atatttttag aattaaggtg aactgtagtg cttaatataa ctataatatt aattgtttga
    21421 atattcaata aaaagtttaa aaaatatcaa ttttgatata aactgaataa atacagttga
    21481 atattaaatt attattttat caaaaatatg caaagtacta caccttcaaa tatcacctaa
    21541 agtaattaaa aggcaattaa agtgaagtag ttc
//
LOCUS       DAEPOZ010000039        21502 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.039, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000039 DAEPOZ010000000
VERSION     DAEPOZ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21502
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.039"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(91..756)
                     /locus_tag="KR440_001182"
     CDS             complement(91..756)
                     /locus_tag="KR440_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698240.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
     gene            1100..2530
                     /locus_tag="KR440_001183"
     CDS             1100..2530
                     /locus_tag="KR440_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBH0698241.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKALKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEDQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEEICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
     gene            2898..3476
                     /locus_tag="KR440_001184"
     CDS             2898..3476
                     /locus_tag="KR440_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBH0698242.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKFVYKKTAIFISTVVLLITILSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            3506..3652
                     /locus_tag="KR440_001185"
     CDS             3506..3652
                     /locus_tag="KR440_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBH0698243.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            3957..5336
                     /gene="scfB"
                     /locus_tag="KR440_001186"
     CDS             3957..5336
                     /gene="scfB"
                     /locus_tag="KR440_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HBH0698244.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            5365..5862
                     /locus_tag="KR440_001187"
     CDS             5365..5862
                     /locus_tag="KR440_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBH0698245.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            6160..7824
                     /locus_tag="KR440_001188"
     CDS             6160..7824
                     /locus_tag="KR440_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBH0698246.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            7837..10287
                     /gene="recJ"
                     /locus_tag="KR440_001189"
     CDS             7837..10287
                     /gene="recJ"
                     /locus_tag="KR440_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBH0698247.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDVSKNTDKLELIFSPNIDKIDLKRYNNIILYDYL
                     YNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEI
                     DLDMVNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNE
                     SLILNNLKNLEKQYNSSY"
     gene            10300..10812
                     /locus_tag="KR440_001190"
     CDS             10300..10812
                     /locus_tag="KR440_001190"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBH0698248.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            10886..13093
                     /locus_tag="KR440_001191"
     CDS             10886..13093
                     /locus_tag="KR440_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBH0698249.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            13118..13567
                     /locus_tag="KR440_001192"
     CDS             13118..13567
                     /locus_tag="KR440_001192"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBH0698250.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNASRPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            13582..14196
                     /locus_tag="KR440_001193"
     CDS             13582..14196
                     /locus_tag="KR440_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0698251.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVSDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            14287..15843
                     /locus_tag="KR440_001194"
     CDS             14287..15843
                     /locus_tag="KR440_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HBH0698252.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNRLDSESK
                     RNFKKLCKENIKKSMFVVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDKKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKETKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEELNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            15833..17095
                     /locus_tag="KR440_001195"
     CDS             15833..17095
                     /locus_tag="KR440_001195"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBH0698253.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            17169..18956
                     /gene="aspS"
                     /locus_tag="KR440_001196"
     CDS             17169..18956
                     /gene="aspS"
                     /locus_tag="KR440_001196"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBH0698254.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTKMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDIQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
     gene            19415..20647
                     /locus_tag="KR440_001197"
     CDS             19415..20647
                     /locus_tag="KR440_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBH0698255.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMKCFGIKDK"
     gene            20661..21467
                     /locus_tag="KR440_001198"
     CDS             20661..21467
                     /locus_tag="KR440_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HBH0698256.1"
                     /translation="MGRKVKIGIIQQHSVLGDVKKNIEKAVEMIDDLGKHGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
BASE COUNT         8624 a         2176 c         3931 g         6771 t
ORIGIN      
        1 acaatgaaaa agaagcttat ctctattttg agacagcctc tttgtatact attattaatt
       61 taattaataa tataatttaa aattataata ttagttaaga tttcctgctt tattagtaaa
      121 taattcttta atagcattgt ttctggaaac taaatcgtta tcagagttgt ttgttttttt
      181 cataatcact gaataacgtt ttttatttat aaaaaatatt aaaatcttat catcatcaaa
      241 attgtattct ttatatctgt caatagactc ataacctaga tttagaatgt ttttgtcttt
      301 tatactatct acatctaaaa tatatgatgg gtcagccttt agtaatatat taattaaaca
      361 tttttcttca ccagcacaga ataactccat cttacttgat atagatttag attgctctga
      421 agttattttt gcaggaataa ggttgtcatt atcatctctt agaagataac catgtgtgtc
      481 ttgttgatat actaaattaa cctttttctt aggaacacta atcattttat ctaaatgtac
      541 ataatgatga ttttcaacaa aaccttcatt taatatttca acaaagttat taattttacc
      601 taggcaaaaa gattctatag attctttaca gaatttattt gtaaatggta ttatgtttac
      661 gttttcctca tctgtattcc atgctatagc ataatccaac gaatatactt cttcctctat
      721 atctgcaaaa ttatattgta ctatgtttcc tttcaaaatt actcgacctc catcttataa
      781 tatatttaat atactttata aaacttatat tatatttaaa taatatcata aattggataa
      841 atatgatata gtaaataaga aaaaatttga aaaaaatcac tataaatgag taataaatat
      901 aaatagtgta tactaatggg atggaaaatg tccactatta actttattac cccacatata
      961 taagataaaa ttattaatat aaaatataat ctatcgacaa tgaatagact tttaaataaa
     1021 cttaatataa aattaattaa ataagtgctt aaaaacgatt tactttaatg atagaatcat
     1081 attaaaggag gataatttta tggatataaa gaataaaatt aaatctatat ttttaccaga
     1141 aaaaaatgac tatttagatg atgaagaatt tgagtatgat atagaaaata atgaagaaga
     1201 tttaaaggaa gataacaaag tacatttaaa tgatttaagc agagtaaaat atgattacag
     1261 agagttaaaa gacatagaaa accaaacaga agagatgtgt ctgctagcag taaagcagga
     1321 tggtactata attgagtttg taaaggacaa gacttataaa gtatgtatgg aagcagtaaa
     1381 acagacatat aaagctctta agtatataac aaatcaaaat gaggatattt gtatagaagc
     1441 agtaaaacag aactacagag ctttgtacta cataaataat aagacagaaa atgtactaat
     1501 tgaggctata aaaaatgcat caacttatga tgtcatggaa gtatttaaat ttgtggaaga
     1561 tcaaacagaa gatgtatgtc tagcatttat agaaagggca tctaaaaatg atgttgcaga
     1621 gatattaaaa ggaataaaag agcaaactcc agccatatgt ttagaagctg taaaaaaaga
     1681 tggaaaatct ttagcttatg taaaggaaca aagtaatagt atatgtttag aagctgtaaa
     1741 agaaaactac agtgcactat cttgtgtaaa agagcaaaca gaagaaatat gtatagaagc
     1801 agtaaaacag aatgactttg cattgtatta tgtaaatgaa cagacagaaa aaatatgtat
     1861 ggaagcagta aaaagaagtt atatggctct acagtatgta aataagcaaa cagaagagat
     1921 ttgtttggaa gcagtaagaa ttgatggaag agcattacag tacgtaaaag agcaaacaga
     1981 agaaatttgc ttggagtcag ttagacaaaa tggaaaagta ttgcaatatg taaaaaaaca
     2041 gactgaaaat atatgtatag aagcagtaag aggttcattt gaagaacttg aaataaaaga
     2101 aatattaagt tatgtaaaga taccaacaga aagaatattt gtagaagcag taaagcaaaa
     2161 tggaaaaatt cttaaatatg tagaaaatca gacagaatta atatgtttag aggcagtaag
     2221 agaaaactat aatgctttag cttatgtaaa ggaacaaaca gaagagattt gtttagaagc
     2281 agttaatcaa agttatgaag cgcttaaata tgtgaaagaa caaacagaag aagtttgtct
     2341 aaaagcagta aaacaagatt atagaatgtt gaaatatgta aataatcaaa cagaaaaaat
     2401 atgtttagaa gcagtaaagc aaaattatag agctttagaa tttgttgata atcaaacaga
     2461 aaaagtttgt cttgaagcag taaaacagaa tagaaaagca ctacagtatg taaaacaaaa
     2521 acaaagttaa aaggctaaaa tatatagaaa atatatgcaa ataaataaaa tttgatattt
     2581 tgtaataaat aaaatgggta tctcaaaatg aactttttag tttgtgaggt actcttttta
     2641 tatgaaataa aatatatttt tagcctttta acaatgggga aaaggtttat ttctattttg
     2701 acataggctt tttatgcata taaagcttga taaaattgtt ttattataat tgttttgtta
     2761 tataaataat aataagaaaa ttatataggt ctttttttga ccagttttgc atttttaaca
     2821 actagttttg taaagtatat tgaattttct caaaaaagaa tgtactctat atatgtaaaa
     2881 tttttttgga ggtaaaaatg tttaaaagat atgctgaaaa aatgacatct gttttaattt
     2941 gtaataatat gatagataat aatgaatcta aagtctattc ttatggattt gaaatattaa
     3001 tagctttcat agttaatata accaccatgc tgtttatagg ttttttattt ggtaaattta
     3061 catatgtatt atttttttta atgtgttatt gtccaataag acaattttca ggagggtatc
     3121 atgcagacaa ttattttaga tgcttattaa cctttatttt tataatttta tctacaatct
     3181 taattataga aaatataaat atagacttat ttaaaaatat tattatgatt attgcaagtg
     3241 ttagttgggt tggaatttgt gttctgtgtc caatagaaca taggagtaac cctataagtg
     3301 atagggaaaa gtttgtttat aagaaaactg ctatatttat atctacagta gtgttattga
     3361 taacaatttt aagtctttct ttgagtatat ttgtagatta tttcacatat agcgcatttg
     3421 ccatgttttg gatttttgtg atgttagtat taggtaagtt aaaagctaaa gtataagtaa
     3481 aatatgtatg agggagagtt aagttatgaa aaagtttatt gtaagattta tgaagtttgc
     3541 tagctcattg gcacttagca ctgctatttt gtcagctaat agtacctgtc cttggattat
     3601 tcatcagcct aaagtaccca aagaaatcag caatctaaag aagacaaatt aaatacaagc
     3661 acttatttat acaaaattat caggaaaaaa gaaattatta tattttaata agattaaaac
     3721 aatataaaat cagatacaaa atttatttta tttattttat aggagcctaa tacctatttg
     3781 gtaatatatt agtgcgccaa tttaggggga tatagtgtat ttaatgaaat gacttacgga
     3841 gagtaagtca tttttgtatt gtatgtattt tatgtaaact tatgatataa tgaagggtaa
     3901 agtttaatta aaataataaa aaactttagt atagatagta atttaggagg tagggtatga
     3961 gtatgataca taaattttct atgaatggat ataatatcgt tttagatgtg aatggtggag
     4021 ctgttcatgt gttagatgat gttgcatacg atttgttaga tttttataaa gaaaaaagta
     4081 aagaagaaat attagagata ttaaaatcaa aatatcaaga agaaaaaata aatgaagcat
     4141 atgaagaaat attaaatttg gaaaaagaag gtcttttata cacagaagat acatatcaat
     4201 atcatccaag ctttgtacat agagagcctg tagtaaaagc tttatgttta aatgtagctc
     4261 atgattgtaa cttaaaatgt aaatactgtt ttgcagctca aggtgatttt ggaggagaaa
     4321 aagaacttat gagttttgaa gttggtaagg cagcaattga ctatttgatt gctaattcag
     4381 gaagtagaaa gaacctagag atagatttct ttggtggaga gcctttgatg aactttgaag
     4441 ttgtaaaaca actggttgac tatggaagaa gtgtagaaaa agattataat aaaaatataa
     4501 gatttacaat aactacaaat ggtgtactgt taaatgatga gattatagat tatataaatg
     4561 aaaatatgca taatgtagtt ttaagtttag atggaagaaa agaagttaat gataatatga
     4621 gaccaacttt aaatgataaa ggaagttatg atattacatt accacgattt aagaaacttg
     4681 tggaaaaaag agcaaaagat aagtattatt atataagagg tacttttaca agagataatt
     4741 tagatttttc aaaagatgtt atgcattttg cagatttagg atttaaatta acctctgtag
     4801 agccagtcgt tggagatgag agtaatccat atgcacttag agaagaagat ttacctaaga
     4861 tatttgaaga gtacgaaaaa tttgctgtag agtatgcaga tagacaatta caaggagatg
     4921 gatttaaatt tttccatttt atgatagacc taaatcaagg tccatgtgta ataaagagaa
     4981 taactggatg tggagctgga aatgagtatc tttctgtaac tccaaatgga gatatctatc
     5041 catgtcatca atttgttgga aatgaagaat ttaaaatggc aaatatattt gatgaagaaa
     5101 tagtacttcc agaaaattta aaaaatatgt ttagagaagc tcatgtatat actaaagaag
     5161 agtgtaaaca atgttggaat aagttttact gttcaggtgg atgtcatgca aatgcgataa
     5221 actttaataa tgacatatca aaaccatatg aattaggatg tgaaatgcaa agaaaacgta
     5281 cagagtgctc tataatgata caagctaagt taatgttaga aggagcaacc aactaaattt
     5341 atattttaaa ggagttgttt atatatgatt agagattatt tagaagataa acctttaatt
     5401 gacgaaagtg tatttgtggc aaaatcagca gatgtaattg gaaatgttaa aataggcaaa
     5461 gattcaagta tatggtacaa tgcagtagtt aggggtgatg aaggaccaat aactattgga
     5521 gaaaacacta atatacaaga ttgttctata gtgcatggag atacagaaac tataattggt
     5581 aacaatgtaa cagttgggca taggtctata gttcatggtt gtaaaattag tgataatgtg
     5641 ttaattggta tgggttcaat aatattggat aatgctgaaa taggagaata tactctgata
     5701 ggagcaggta cactaattac atctaataag aagtttccac caggtgtttt gataatgggt
     5761 tctcctggta aggttgttag agagcttaca gaagaagata aaaaatatat tgatgaatct
     5821 tatgaatggt atttagaggc agcacaaaat caaaaatatt agtttaaaga gtggttgttt
     5881 caaaacatat ttaaatctgt tttgaggcaa ccactaattt attttattag attttgttga
     5941 tattttagtt ttcaaaaagt atttgattaa accagatata aaatgtcaaa aataaaaatt
     6001 aactgagtta aataatttta ataaaattgg tagtatatgt gataaaatta aatattaaag
     6061 aggatatagg atattagaat atcaattggt gaaataatgt catatacgat aaattaaggt
     6121 gtaagttttt gggtatacct atcttaggag tgaaaagatt tgagaataag ttatagaaat
     6181 ttaaagcgat atagagaaat tggatatgtt ttaataaagt atggttttag ttttattgta
     6241 gaaagactaa atatagaagg aattgcctat aaaataccac tatttgaccc accagaagaa
     6301 ataaaaaata tgacaactgg tgagagaatg aaaagagttt tagaagaact aggacctaca
     6361 tatataaaaa taggacagat attgagtaca agaaaagact tgttagacca ggatataatt
     6421 gatgaaatat caaaactcag agatgatgta gagaaatttg attcaaatat agctattgat
     6481 atatttaaag aagaagtggg tttaagtata gaagaaatat tcttagaatt taaagaagaa
     6541 ccaatagcag cagcttcaat tggtcaagtt tatgaaggtg tacttaaaac gggagaagaa
     6601 gtaatcgtaa aaattcaaag accaaatata gagaaaataa taaaatctga tttagagatt
     6661 cttagaacta ttgcaaatac tttaaaagat ttaaaaaagg attttaattt agatttagta
     6721 cagatgattg aagaatttca aactcaatta atgagggaac ttgattatac atttgaagcc
     6781 ataaatgcaa ctaaattctc taggatattt aaaaatagtg atgaagtata tatacctaaa
     6841 gtatactcag aatataatac taagaaaatt ttagtaatgg agaaagttaa tggtacaaag
     6901 ttaagtgatg tagaaaaaat aaggagacta ggttataaca caaaaactat tgttgaaata
     6961 ggagttaggt cattttttac acaggtatta tctcatggat tttttcatgc agacccacat
     7021 ccaggtaata tatttgtagt tgctaaaaat aagatagcat atatagattt tggaatgatt
     7081 ggaattattg ataataaaac tttaaaccaa ttaaatgaaa ttgcattagc aggtgtagaa
     7141 aagaatgttg acaagataat atatctattg atagaaatgg atgcattaaa tggagaggct
     7201 gatattaagg gacttagaca ggatttactt tatctaatac attattacta tgatatttct
     7261 atagaaaaaa taaatgtaac agatatatta aatgaactat ttaggttttt tagacagtat
     7321 aaaatagtca tgcctgcaca atttgtaact cttgcaaaga cagttataac attagaagga
     7381 acaagtagaa ctttaaacac agatttttct tttggttcta tgggaaaaga gtttatgaaa
     7441 catcattata agagtaaatt taaccctaaa aatgttgttt taagttcaag acaaaatgta
     7501 gaagaaatat tactggacat aaaaactatt ccaaagcaat taaaagcaat tttaaaaaat
     7561 atagaaagaa ataatataaa aatgcaaatt gaagatgtta aaatgacgag gttagaaaat
     7621 tgtataatag agctaacatc tcaaatatct ttgagtttag tgttggcatc tattatagta
     7681 ggttcttctc ttataattgc atctccaaat atagaaaata atatatggat aaaatttact
     7741 gcaatagcgg gtttctttat atcctttata ataggccttt gcttagttat aagaagtatt
     7801 aggtcaaaat acaaaaaaga ttagggggaa ttaaatttga aaaaatggat attgaagtat
     7861 aaaggtaaga tagaggaaag tgaatttagc aaaaaactta atatttcccc ggaaatctgc
     7921 caaattttaa aaaatagagg gatatgtact gaaaaagatt cagaaatatt tatgaatcca
     7981 tctttagatt atttaagaga ccctttttta atgaaagata tgcaaaaagc tgttgataga
     8041 ataaagttag cagttgagaa gaacgaaaaa atttggattt acggagatta tgatgttgat
     8101 ggtgtatcat ctacatccat cttgtgttta tattttaaga gtataggtta caatgttgat
     8161 tactatattc ctaatagatt agaagaaggg tatggtataa atgaggatgc tattaagctt
     8221 ataagctcta ggggatgtga cttgattata agtgttgact gtggaattac atcagtgtct
     8281 gaggtcaata cagcgaatga tttgggcata gatgttataa ttacagacca tcatgaatgt
     8341 caaagtgaaa ttccatcagc ctttgcagta ataaatccaa aacaagaaga ttgtaattat
     8401 ccatttgatt cactatgtgg atgtggtgta gcatttaaaa tgattcaagc cttgactcca
     8461 aaagaagaat ttaaaactag tatgtataat tatttggaaa tagtaacact agcaactatt
     8521 tgtgatattg ttccacttat agatgaaaat agaataattg taaaaaatgg tttaaaatca
     8581 atgaaagaag gcaaaaatat tggattaagg gagcttataa aagtttgtgg tgtagaaagt
     8641 gataagatag gttcttctca tataggtttt gcaataggtc ctaggataaa tgcttcaggt
     8701 cgattgggat attcttactt aggagtagag ctatttacta ctcagtcaca agaagaggca
     8761 gtagaaatag ctagtatatt ggaagaaaaa aataatgaaa gacagatgat agaagctaag
     8821 atgtatcatg aagctgaaga aatgctaaag tctaatagca gatacaatga tgacaaagtg
     8881 ttggttttag ccaaagaggg ttggcaacat ggaataatag gaattgtagc atcaaagtta
     8941 actgaaaaat actacaaacc aacaattctt ctaggtatag aaaatgggga ggctactggt
     9001 tcagcgaggt caataaaagg atttaatata tttgaagctc tcataaagtg caaagacttg
     9061 atgactaagt ttggtggtca tgaacaagca gcagggttat ccttggattc agacaatgta
     9121 gaaattttag caaatgaaat aaataaattt gcagattata atttaactga agatgatatg
     9181 atagagaatg taaatgtcga gtttgaatta caagaaaatg taataaattt aaatcttgta
     9241 gaagaattac ataagttaga accctttgga ttaaataatc ctaatcctag atttatagtt
     9301 agaaattata ttttaaaaga tttgaaagtt ataggtaaga atcaacaaca tctgaaatta
     9361 agtatagaaa aagagaaaag ctatgaatgt ataggattta atatgagtca tttaaaatca
     9421 atgtataaag taggagacaa agtagatgta ctattccaat tagatgaaaa taactatatg
     9481 ggaaatagga aagtacaatt tttacttaaa gatatacgat tggctcgacc taaaagtgca
     9541 agtaatgaca aactttcatt gaaattaatg tcaaaaataa taccaaaaga tactcaaagc
     9601 ctgtataata tatctgtgtc tgattttgaa ttatttgatg gaaatacaga tataaatata
     9661 tttgattatt ttgaaaaaga tacactaata atatctaatt ctataaatgg attttataga
     9721 gcaatgtcag atatatcact tatagatttg gattttaaca taaattacaa tattattgaa
     9781 gatgttagca aaaatactga taaattagag cttatatttt ctcctaatat tgataaaata
     9841 gacttaaaaa gatataataa tattattctt tatgattatt tatacaataa aggcgaatat
     9901 tcgtatatat atgagaataa aagagaagaa agtgaaatta taaaatatta taataaaaca
     9961 gacctattat atttaaagaa tgtagttagt aatatagttc catcaagaga tgaatttata
    10021 acaatttata aacaagcact tataaaaaaa gaaattgatt tggatatggt aaatataagg
    10081 gaaactttta atgtaattcc attaaagttt tttacgatat taaatgtatt tagagaatta
    10141 aatttacttg actttaattt aaattatgaa aaaaattctg tactcataag aatattgcca
    10201 aaaccacaga aaaaattaga cttaaatgag agtttaatac tcaataatct aaaaaattta
    10261 gaaaaacaat acaatagcag ttattaggag gcgtttccca tggatttaaa aaactttata
    10321 aggaatattg atgatttccc aaaaccaggc atagatttta aagatgtgac aactctattt
    10381 aaagatggag atgcatttaa atatgcagtg gattctatag ttgaagaatt gaaagataaa
    10441 gatgtagatt tagtaatagg gccagaagca agaggatttt tgatgggtac acctgtagca
    10501 tatgctttag gagtaggatt tgttccaatt agaaaacctg gaaagttacc aggtgaagtt
    10561 gagagttatg agtatggatt agagtatggt acagatactt tagagataca taaggatgca
    10621 attaaaaagg gtcaaaaagt agcaatagta gatgatttat tggctactgg tgggactatg
    10681 gaagccgctg ccaagcttgt agaaaaatta ggtggagaag tagtttctat gcaatttttg
    10741 attgagctaa aattcttaaa tgggagagaa aagttgtcta actatgatgt taattctctt
    10801 ataaaatact agtactttta taatagttgg tataacacca actattttca tatatattaa
    10861 aattttatac aaaataggtg gttatatgca tgataaagaa ttacaagaaa taattgaaaa
    10921 aataaaagtt tatgcaccta atgcaaattt tgaaatggtt gaaaaagcct ataatcttgc
    10981 aaaaaaagcg catgaaggac aatatagaaa atctggggaa ccatacttta ttcatccatt
    11041 agcagttgca aatattttaa tagagatgga attagatatt gaaactataa cagcaggttt
    11101 acttcatgat gttgtagaag atacagaata tacctatgaa gacataaaaa aagaatttgg
    11161 caaagaagta gcggatttgg ttgatggagt aactaaactt ggtcaaataa aatatcgttc
    11221 gaaagaagaa actcaatctg aaaatttaag aaaaatgttt ttggcaatgg cgaaggatat
    11281 aagggttata cttataaaac ttgctgatag attgcacaac atgagaacat taaaatacat
    11341 gcctccagaa aaagcaaagt ataaagcaaa agagacactt gaaatttatg gtggtatagc
    11401 tcatagatta ggaatttcca agataaagtg ggaactagaa gacagagcat taagatttat
    11461 ggacccagaa gggtattatg atttagttag tagagtatca atgaagagaa gtcaaagaga
    11521 ggattatatt caaggaattg ttgaacttct taaagataag tttaaagatt taagtatacc
    11581 atgtgatgta tatggtagac caaagcattt ttacagtata tatagaaaga tgcaaaaaaa
    11641 acataagact tttgaagaga tttttgattt aacagcagta aggatattgg ttgataatgt
    11701 aaaggattgc tatgcagtac ttggtatagt tcatacatta tggagaccta ttccaggaag
    11761 atttaaagat tatatagcaa tgccaaaacc caatatgtat caatcattac acaccactgt
    11821 tgtaggtcct gatggagaac ctttagagat acaaataaga actcatgaga tgcataatat
    11881 agctgaaaat ggtatagcag cccattggaa atataaagaa ggtatctcta gcaaagagtc
    11941 aaaggttgaa gaaaaacttc aatggttgag acagatgatg gagtgggaaa aagatttaaa
    12001 agacccacaa gagtttatgg atgcacttaa agaagatgta tttaatagtc aagtttatgt
    12061 atttacgcca aaaggagacg taattgaatt accagcagaa tcaacaccta tagattttgc
    12121 ctatagagtt catacaaatg taggtaataa atgtgtagga gctaaaattg atggaagaat
    12181 agttcctatt gattataagc ttcaaaatgg taatattgtc gaggtactta catcatctaa
    12241 ttctaatgga ccaagtaggg attggcttaa tatagtgaag accccaaatg caaaaagtag
    12301 aattagacaa tttttcaaga aggaaagaag agaagaaaat gttgaccgtg gaaatacaat
    12361 attagaaaga gaatttaaga agcatgatat accacaaaaa gaccctcttg ttgaaaaata
    12421 tatggcatta gttgctaaga aatttaatca gccaagtgtt gaggacttaa ttgccactgt
    12481 aggttatggc ggtataatgt catctcaagt ggtatctaag gtaaaagacc tatatttaaa
    12541 agaggttaaa aaagctaaaa aggaacaaca gatacaagaa gaagatttaa ataaacacaa
    12601 tataagtgaa acagaataca gtaaaaagag aaagaaaaac gcatcacaag gaattatagt
    12661 aaaagggctt gacaatatct tagtaagatt tgctaagtgt tgtaatcctc ttccaggaga
    12721 tgaaattgta ggatatatta ctaagggtag gggagtagcc atacacagag ctgattgccc
    12781 aaatgcaaac aataatggtg atttcttcca aaatagattg gtggaggttt catggagttc
    12841 agcaggaaaa ggtaagtttg aagcggaaat tcaaataaaa gctattgaca gaagaggaat
    12901 tgttaatgaa ataactcata ttgttgcaat ggataaactt agtttaaacg gaataaatgc
    12961 aagaaaaggt aaggatagta ttgtaagtat aaacttactt gtagaggtta atgacacaga
    13021 ggaattaaaa attctaatga aaaaattaaa agcaattcct ggtgtagaaa gtatttatag
    13081 aatgacaaat tagagttaaa ataataggga ggaaactatg agagcagtag tgcaaagagt
    13141 atcttcatct aaagttacag tagatgaaaa cactataggt caaataaata aggggttact
    13201 agtattgtta ggagtgactc atgatgataa atcaagtgat gtagattata tgattgataa
    13261 aatattgaat ctgagaattt ttgaagatga aaatgacaag atgaatttat ctctaatgga
    13321 tataggtgga gagcttttag tagtatctca atttacttta tatggagatt gcagaaaagg
    13381 aagaagacct ggatttagta atgcatctag accagaactt gcaaataacc tttatgaaga
    13441 atttgtaaaa aaagcaaaag ataaaggtgt gacagttggt acaggtcaat ttgcagctca
    13501 tatgatggta gagttaacta atgatggtcc agtaacaata ctattagact caagtaaatc
    13561 tttttaaggg ggaaaacttt aatgattata aaaaaatttg tagatagtta ttttggagta
    13621 aatacttatg tattaggtga tgaaaagact aaaaaatgtg cagttataga cccaggtgga
    13681 agtttagtag agacattaag ttttgtaaaa gaaaatgaat taaatattga atatattatc
    13741 cttactcatg ggcatggaga ccatatagga tatgttaagg atattaaaga aaaaactggt
    13801 gctaaagtag ttgcacatgt tgatgaaaaa gaattattaa atgataaaaa taaaaatcta
    13861 agttatacaa tgagatgtgg agcgcaagag tttgatgcag atatctatgt gagtgataaa
    13921 gacaagcttg aacttggtga attaaaaatg actttcttgc atactccagg acatactcaa
    13981 ggatgtatgt gtataagagt tgaagatgaa atgttcacag gagatacttt gtttgctgga
    14041 agtattggta gaactgattt atatagtgga gactttaacc aaatggaaaa atcacttaag
    14101 aaattatgca aatatgaaga tgctgttaga gtatatcctg gacatggacc aaccagtact
    14161 ctaggggttg aaaagaagac aaatccttac atgtaatttt atattagtat ttaaaaaagt
    14221 ataaaatatt taataactat attagtgagg gggttcttct actgttaaat agaaggagtt
    14281 aagttgatgt tgtatgtttt tttaaaagga catgattata agtatgaagt agcagaactt
    14341 ataaaattgt ttaccagtga atttaagttt aaagactcta atgctaacaa taatatagag
    14401 tcaaagcatt taataaatag attgatttat gagaatggtg tattattctc aagtactgaa
    14461 tactatgaaa atggaaattt aaagtacgag tcaatacaga atatagaaaa tatgaatctt
    14521 gaatttttag atggatttga tagtagcaaa acgattgaag aatttcttaa tagactagat
    14581 agtgaaagta agaggaactt taaaaagctg tgtaaagaaa atatcaaaaa aagcatgttt
    14641 gtagtgctta aacaagtatt taattcttat gtaccatggg gaattttgac tggtattaga
    14701 ccagtcaaaa ttgtccacaa tttaatggat aaaaagctag atgataatag cataagaacc
    14761 attctaaaag ataattattt tataatagat aagaaaatag atttagctct tgaaatagct
    14821 aaaagagagc gattatttat gtatcctata gataaaaata aaatttctct ttatgtaagt
    14881 attccatttt gtccaactag atgtgtatat tgttcatttc cttcaaattc attaaaacaa
    14941 tttggacatt taaaaagaga atatgtttat aaattgattg aagaaattaa ggggtttgcc
    15001 aaagttatta aggagactaa gaaagaagtt gaaactctat atattggtgg tggaactcca
    15061 actacacttg atgaggaaga attagatttg cttataaatt cattgttcaa tgaattagat
    15121 ttaagtaaaa ttaaagaatt tactgtagaa gcaggtagac ctgacactat tacagaacaa
    15181 aaactaagag tgttaaaaaa acacaatgtt agtagaatta gtataaatcc tcaaactatg
    15241 aatgatgata ccttggtaaa gattggaaga gagcatagag tatctgattt agtagattgc
    15301 tttaatatgg ctcgtagaat gggatttgat aatataaata tggatataat tttaggtctt
    15361 atagatgaag aattaaatat ggtaaagaac acattggagg agataaaaaa actttctcct
    15421 gaaagcctta cagttcatac attagccata aagagagcat ccaacttaaa tataaatatg
    15481 gataggtata aagaacatct aacacaatat gaagaaatgg taaaaatgat agatttatca
    15541 atgaaatatg caaaagatat gggattaaat ccatattata tgtacagaca aaaacatatg
    15601 ttgggaaatt tagaaaatat aggttatgct aaagaaggat atgagtgcat ttataatata
    15661 cagattatgg aagaaaaaca aagtaactat gcattaggtg caggtgctat atcgaaattt
    15721 gtttatgtag atgaagatag aattgaaaga gtagaaaatg taaaaaatgt tgaacaatac
    15781 attgaaagag tagatgaaat gataaaaagg aagaaagaag aggtatataa aaatgttgac
    15841 taaagctcct agaggaacta aagacataac tccaaaagag gcttacaaat ggcgttatgt
    15901 agaaaataaa tttagagaaa tatgtgcttt atatggatat gaagaaatgg taacacctat
    15961 atttgaacat acagaacttt tcaagagaag tgttggagat actacagata tagttcaaaa
    16021 agaaatgtat tcttttaaag ataaaggtga tagagaaatt accttaaagc cagaaggtac
    16081 agcaggtgta gtaagagcat ttatagaaaa taaattatat gcagatactc aaccaacaaa
    16141 attattttat gtgacacctt gttttagata tgaaagacca caagctggta gacaaagaca
    16201 atttcaccaa tttggaatag aagcacttgg aagtgatact ccttcaatgg atgctgaaat
    16261 aatcgcacta gctgttcaat tttttaatga agttggatta aatgatttgg tagtgagtat
    16321 aaattcagtt ggctgtccag tttgtagaaa agagtacaat gctttattaa aagagtatct
    16381 tgactcaaaa gctgatatat tgtgtgatac ttgcaatgag agaagagaaa aaaatccaat
    16441 gagggttata gactgtaaaa atcctacatg taaagaaaat atcaaagata taccatttat
    16501 tgcagaccat ttgtgtgatg attgtaaatc tcactttgat aaacttcaag aatacttaaa
    16561 ggaaatgaat ataaactttg taattgataa aacaatagtt agaggcttag attactatag
    16621 aaaaactgct tttgaaatta tatctaatga tataggagct caaagtactg tatgtggtgg
    16681 aggaagatat gatggtcttg ttgaacaatt aggaggacca aaaggtataa gtggaattgg
    16741 gtttggacta ggaattgaaa gacttttact tacacttgaa ggtaatggaa ttgaaataga
    16801 aaaccctcaa tctacggata tatttatagt aactataggt gaagaagcaa atacaagaag
    16861 ctttaaatta ttaaaagact tgagacaaaa tcatataagt gcagataaag accatataga
    16921 gagaagtgtt aaagcacaat ttaaatattc agataaaata aattcaaaat ttacaatagt
    16981 gattggagat gatgagctta aaaatgacac tgcgacatta aaaaatatga aaacatcaga
    17041 gcaaactact gtaaaattaa gtacattagt tgaagaatta aagcaaaagc tgtaaaataa
    17101 gaaagtatga tgttttgtgt attagataaa tatatagcat taaaattaaa taaaatggag
    17161 gtagtatctt ggaaacttta aagggtttaa agagaactca ctactgtgga gagttgagag
    17221 aaaaaaacat aaatgaagaa gtcgtactta tgggatgggt acagaaaaaa agaaatttag
    17281 gtggtcttgt atttgttgat ttaagagata caagtggatt atgtcaaata gtttttgata
    17341 cagatgtaga taaagaagct tttgaaaaag ctgaaaaatt aggggctgaa tttgtaatag
    17401 ctgtaaaagg aaaagtatgc gaaagacaat ctaaaaaccc taatatgcca actggtgata
    17461 tagaaatatt tgcaactgaa cttagacttt taaacaagtc agaaactccg cctatttata
    17521 taaaagatga tgatgatgtt tctgaagcac taagattaaa atatagatac ttagatttaa
    17581 gaaaaccatc tatgcaaaga aatttaaaat taagacataa agtaatgaat attacaagaa
    17641 attacttatc aaacaataga ttttgcgaaa tagaaacacc atttttaata gctccaacac
    17701 cagaaggagc aagagattat ctagtaccaa gtagagttaa tccaggaaaa ttttacgcat
    17761 taccacaatc accacaatta tataaacaat tattgatggt aagtggtatg gataggtatt
    17821 tccaaatagt taaatgcttt agagatgaag atttaagagc agatagacaa ccagagttta
    17881 cacaaataga ctgtgaaatg tcttttgtcg agcaagaaga tgttatgagt atgattgaag
    17941 gtttgcttga agcgatattt aaagaagttt tagatgtaga attggcactt ccacttccta
    18001 agatgactta tgcagaagct atgtctaaat atggttcaga taaaccagat actagatttg
    18061 gatatgagtt aacagatata tctgatgttg tttgtaactg tggatttaaa gtgtttgctg
    18121 atgctacaca accaggaaaa agtgttagag gtataaacgt aaaaggcaaa gctgacgact
    18181 ttactagaaa acaaatatca tcactagaag aacatgctaa gacatataga gcaaaaggtc
    18241 ttgcttggat gaaagtagga caagaaggag taacttctcc aatagcaaaa ttctttaatg
    18301 aagaagaaat gaacgctata cttacaaaaa tgaatgctga agtaggagat ttacttttat
    18361 ttgtagctga taaaaactca atagtatttg atgctttagg acaagttaga cttgaagtag
    18421 caaataggct taatctattg gataaaaatg tatataactt actatgggta actgagttcc
    18481 cagtttttga agaagatgaa gaaacaggca cattttcagc aatgcatcac ccatttacat
    18541 cacctatgga tgaagattta gacaagttag aagaaggtga taagtcatct ttaagagcga
    18601 aagcttatga tattgtattg aatggttatg aaataggtgg aggaagtgtt agaatatcaa
    18661 attctgatat tcaatcaaga atgtttaaag cacttggttt cacagaggaa agagctaatg
    18721 aaaagtttgg ttatttatta gaagcattta aatatggtac tcctcctcat gctggtctag
    18781 cttttggtct tgatagactt gttatgttac ttgcaggaac tgataatata agagaagtta
    18841 ttgcattccc taaaaatcaa aatgctgttt gccctatgac aaatgctcct acattggcgg
    18901 aagatgagca attagaagaa ttaagtataa aagttgatat aaaagataat gaataatatg
    18961 tagttgcaag aaactaaaat gtaaaaagtc tggatataaa gtatatttat attcagactt
    19021 tttttattag aattatttta tattttttct ctatatattt tcataaatta tccttgtatt
    19081 ctcagccaat ataattaaaa tactatgttt aaaaatttta cataaacttg attgttttag
    19141 aaattacttc tgtaatgagt acacgatttt aattaaagaa tatataacaa atttgaaata
    19201 agtctaatat atgacttaat agattaaatt aaaataataa atttggacta tttatctatt
    19261 tataacttaa attatatcaa attgataaca aactagatat tatacttttt tgtagaatag
    19321 aattacaatt atataaagaa aaattctatt aatagtgaaa aaaaagtaga aaaaagtaga
    19381 aattattctt ttataaagaa agggagggat aatattgcaa gagagagaag aattagtttt
    19441 aaatccagtt ccaaaagagg aaagagttgg atggttagct ccattgttta atatgcttgg
    19501 ttctaacata gctatatctg aactaatggt tggaggaact ttaattttag gaatgactct
    19561 ttctaatatg attattacat caattatagg gaacttaata ttagttgcaa tcattatgat
    19621 acaaggttat ataggttaca aagaagggtt aaatacgtat gtattagcta agggggcatt
    19681 tggagagata ggaggaaagt atcttatatc tctattactt ggtataacta gttttggatg
    19741 gtttggtgta caagcagggg ttgcaggact atcagtacaa aagatattcc caagtgtaaa
    19801 cctaacatta gttacagtta tacttggatt atttatggtt gtttttgcac tgtatggctt
    19861 taaagctatg gctaaattta attacttagt tataccacca cttatgatat taatggtgtg
    19921 gggagttttt aaagcttttt ctacttatgg agttgaagct atttataatt atactcctca
    19981 aacaactatg agtatggttg aaggtcttaa tattgtagta ggtcttgtta tagttggtgc
    20041 aattatatca cctgaccagt taagatatac gagacgagta aaagatattt ggattatcag
    20101 ttttttaggt ctagggatta tttcattatt ccaacaagtt gcagctggtg taatgtctat
    20161 gggagcacct acttgggata taacagaagt attggctaat ttaggttttg gatgggtagc
    20221 gtttgttatt ttaatacttg catcttggtc aacaaacgta tcaaatgctt attctggtgg
    20281 attagcatta aaaactattt ttccaaatgt aaaaagaaat gttttgactt tagttgcagg
    20341 tctaataggt acaataatag cagctactgg tataatattt aaatttcagt catttttatc
    20401 tttcttggga atcgcagttc ctgcaatagc aggaattatg tggtgtgagt attattttat
    20461 acaaggaaga acttataagc atagagaagg aataaattgg attgcagtaa taagctggtt
    20521 aataggtttt gcagcgtcgt attatagttc aaaaataaat ttcttaatac ctccgataaa
    20581 tggaattgta gtgagtatgg ttatttatta tatattaatg aaatgttttg gaatcaaaga
    20641 taaataaagg gggcaattac atgggtcgta aggtgaaaat aggtataatt caacaacaca
    20701 gtgtacttgg agatgttaaa aaaaatatag aaaaagcagt agagatgata gatgacttag
    20761 gaaaacatgg ggcagatata atttgtttac cagaactatt tgctacagga tataatttag
    20821 agtctttagg tggagttaaa acattagaat taataagaga acataataag tacatagagg
    20881 aatcaatgtc agaagctgct aaaagaaata atgtatacct gatatctcca tatggaacct
    20941 tagaaaaagg gtctacacat gtttataatt cagcagttat atttgataga aaaggtaaga
    21001 ttatgggaga atactgtaaa aatcatctgt ggtcattaga agcagtatac tttaaaggag
    21061 gggaaaaagt agaggtatat gatgctgatt ttggtagatt tggtgtaatg atatgttatg
    21121 atgcaggttt tccagaagta tccagagaat tgacattaaa aggttctgaa ataatattta
    21181 taccatctgc atggagaata caagatgaag atatgtggga tttaaatgta tctcaaagag
    21241 ctttagagaa cactgtatat acagtaggtg taaatttagt aagtaatgac tctaatttaa
    21301 tactatttgg aaagagtaaa atatgcaatc ctagaggtac tgtaataact caattagata
    21361 catacaggga aaaatatgta ttatcagaga ttgatttaga tgaaataaaa aagtataggg
    21421 aagatatacc ctatttgaaa gacagaaaaa gtagctataa catataattc tatactataa
    21481 tacaagttca atataaaatg at
//
LOCUS       DAEPOZ010000040        21281 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.040, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000040 DAEPOZ010000000
VERSION     DAEPOZ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21281
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.040"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            88..1302
                     /gene="dpaL"
                     /locus_tag="KR440_001199"
     CDS             88..1302
                     /gene="dpaL"
                     /locus_tag="KR440_001199"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBH0698257.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLRDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            1635..2822
                     /locus_tag="KR440_001200"
     CDS             1635..2822
                     /locus_tag="KR440_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBH0698258.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGINAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYNGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGAEGYYGILKSV
                     YKK"
     gene            2838..4421
                     /locus_tag="KR440_001201"
     CDS             2838..4421
                     /locus_tag="KR440_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBH0698259.1"
                     /translation="MRTLIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKSLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            4449..5777
                     /gene="ssnA"
                     /locus_tag="KR440_001202"
     CDS             4449..5777
                     /gene="ssnA"
                     /locus_tag="KR440_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HBH0698260.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            5976..7328
                     /locus_tag="KR440_001203"
     CDS             5976..7328
                     /locus_tag="KR440_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBH0698261.1"
                     /translation="MSQDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            7520..8629
                     /locus_tag="KR440_001204"
     CDS             7520..8629
                     /locus_tag="KR440_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0698262.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            8912..10303
                     /gene="hydA"
                     /locus_tag="KR440_001205"
     CDS             8912..10303
                     /gene="hydA"
                     /locus_tag="KR440_001205"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBH0698263.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            10297..12858
                     /gene="xdh"
                     /locus_tag="KR440_001206"
     CDS             10297..12858
                     /gene="xdh"
                     /locus_tag="KR440_001206"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HBH0698264.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETASLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSVPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            13010..14356
                     /gene="rpoN"
                     /locus_tag="KR440_001207"
     CDS             13010..14356
                     /gene="rpoN"
                     /locus_tag="KR440_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBH0698265.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
     gene            15153..16175
                     /locus_tag="KR440_001208"
     CDS             15153..16175
                     /locus_tag="KR440_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBH0698266.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            16235..17242
                     /gene="gap"
                     /locus_tag="KR440_001209"
     CDS             16235..17242
                     /gene="gap"
                     /locus_tag="KR440_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBH0698267.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            17464..18666
                     /locus_tag="KR440_001210"
     CDS             17464..18666
                     /locus_tag="KR440_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBH0698268.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKANNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            18692..19435
                     /locus_tag="KR440_001211"
     CDS             18692..19435
                     /locus_tag="KR440_001211"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBH0698269.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLSK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            19444..20976
                     /locus_tag="KR440_001212"
     CDS             19444..20976
                     /locus_tag="KR440_001212"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBH0698270.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFANKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAIKAVETVDTCVGKLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLI
                     SK"
BASE COUNT         8589 a         2452 c         4268 g         5972 t
ORIGIN      
        1 tattggcata gtaattgctt cactataaga cacaaagata aaaaattaaa atgacaccga
       61 aaaatttgaa aggggaatat aaaataaatg agagaaataa aatggaaatt aaataactta
      121 cctaaatcag aggaaaaaga aaaaggaatt gagtttttaa gagatgaaga aattgcaaag
      181 gctaaagctt ttcatgagag ctttccacaa tatgaaaaaa ctcctttagt taaattagat
      241 aacttagcaa aatcactagg cgtaagcgga atatatgtta aagacgaatc ttatagattt
      301 ggtttaaacg catttaaagt tttaggtggt tcttactcta tgggtagata cttagctcaa
      361 agattagata cagatatatc tgagttaggg tatgacaaat taacttcaaa agaaataaaa
      421 gaaaaattag gagaaataac tttctttaca gccacagatg gaaatcatgg tagaggagtt
      481 gcttggacag ctaataaatt aggtcaaaaa tctgttgtat taatgccaaa aggttcttct
      541 gaatttagat taaataaaat caaaggagaa ggtgcagatg ctagtataac agacttaaac
      601 tatgatgatg cagtaagatt agctaatgat tatgcagaag ctgatgacca tggagtaatg
      661 gttcaagata cagcatggga cggatatgaa gaaataccag catggataat gcaaggatat
      721 ggaactatgg ctaaagaagc tatagaacaa ttaaaagaat atggagtaga tagaccaaca
      781 cacgtatttg ttcaagcagg agttggttca cttgctggtg cagttcaagg gtatgttgct
      841 tcaatatatg atgaatgccc aataacagta gtagtagagg ctgatgaggc tgattgttac
      901 tataaatcag cagaagctgg agatggaaaa ccaagatttg ttggaggaga tatgccaact
      961 ataatggcag ggcttgcttg tggagaacca aacactatag gttttgaagt gttaaaaaat
     1021 tattcatcag cattcgtttc agcaccagac tgggtatctg ctaaaggtat gagaatatta
     1081 ggaaaccctc taagaggaga tgagaaagta atctctggag aatctggagc agttacaact
     1141 ggagcattag tttcaatcct tgaaagtgaa gatttaaaag acttaagaga agctttgaaa
     1201 ctagatgaga actctaaagt attattaata agtacagaag gagatactga ccctgataaa
     1261 tatagagata tagtttggaa tggtgaatgc caaagtaaat aaaagactta aggaaaataa
     1321 aaacttaaca aataaatgaa aaatttaagg aaaataaaaa cttaaataag tgaaaacttt
     1381 aaataaaata aaaactaaaa caaatgaaaa atctaaataa atgaaaaagt aaagcaaatg
     1441 aaaaattcaa aataaaaact aaataaaatt aaataaaacc aaataaaatt aaaataaagt
     1501 ctaaataaaa atttaataaa taaaatataa agaaataaaa aatcaataga ttaagtgaaa
     1561 tgctaataaa ttaaaaaagt gaaatatcaa aataaaacgt caacaaatta aaaattatat
     1621 gggggaatat caaaatgtta aatgaaacaa gaaaaaaaca attaactgaa gtatgccaag
     1681 acttaataag aaatgctagt tattcaggtc aagaagaaaa cgtagtaaaa gctatagaag
     1741 aaaattttgc tttactaggt tttgatagtt ggtcaagaga tagatatgga aatatagtag
     1801 gatgtataaa aggaaataaa ccaggtaaaa aaatattatt tgacgggcat atagatacag
     1861 ttccagttcc agatgcaagt aaatggagtg ttcctccttt tgaagcgaga atagtagatg
     1921 ggaaaatata tggtagagga acttctgata tgaaggggca agtaagtgct atgatggctg
     1981 ctgtttcata ctttgcagaa gatactaaca aagactttga aggtgaattg tatgtagcag
     2041 gtgttgttca tgaagaaata tttgaaggtg tttcagctag agagataagt aaggctgttc
     2101 aacctgatta tgttgtaata ggtgaatctt cagaattaaa cttaaagata ggtcaaagag
     2161 gtagaggaga aatagtagta gaaacttttg gtaaaccagc tcactctgct aatccagaaa
     2221 aaggtataaa tgcagtttat aaaatggcta atgtaatcca aaagatacaa caattagtac
     2281 ctccaactca tccagtttta ggtgatggaa tattagtttt aacagatata aaatcttctc
     2341 catatccagg agcatcagta gtgccagatt attgcaaagc tacttttgat agaagattat
     2401 tagttggaga aacaagagaa ggcgtattag ctccaataca agctttatta gatgaaatga
     2461 tgaaagaaga cccagaatta aatgcaaaag tttcttatgc agttgaaaaa gctgattgct
     2521 atacaggaaa tactatagaa agtgaaagat tcttcccagg ttggttatat gatgaagaag
     2581 atgaatttgt tcaagcagcg tataatggat taaaagaagc tggaatagat tcagaaataa
     2641 ctcaatactc attctgtacg aatggaagtc actacgcagg tgaggctgga ataagaacta
     2701 taggatttgg tccttctaag gaaaatcttg ctcatacaat agatgagtat atagaattag
     2761 accaattatt caaaggtgca gaaggatatt atggtatatt aaaatcagtt tataaaaaat
     2821 aattaggaga tggttttgtg agaactttaa taaaacatgg gcttatagta gatggaaata
     2881 aaacaccagc atatgaagga gatattttaa tagaaaacga gaaaatatta aaaatatcac
     2941 aagatttaaa cgaagaagct gacaaagtta tagatgcaaa aggtagagtt atatgtccag
     3001 gtttcataga tactcatagc cattcagacc tagttatttt agttaatcca tataatgagg
     3061 taaagattag acagggaatt acaacagaag tgttgggcca agatggtata tctatggccc
     3121 ctctacctca agagcatata tcttcttgga gaaaaaattt agcaggtcta gatggtgaaa
     3181 gtgatgaaat tgattggaaa tatgaaacaa cagagaatta tttaaaaatg atggattaca
     3241 atggggtagg tttaaatgaa acttatttag tacctcatgg aaacgttaga atggaagcta
     3301 tgggacttga agatagacct gctactaaag aagaaattca aaaaatgtgt gaaatcacag
     3361 agagagaatt aaaagcagga gctataggat tgtctactgg acttatatat ataccttgtg
     3421 catactcatt gactgaagaa attatagaga tgtgtaaggt agtagcaaaa tatgacggag
     3481 tatttgtagt tcaccaaaga agtgaagcag atacaatttt aacttcaatg gaagaaatca
     3541 tagaaattgg aaaacaatca ggagtaaaag ttcatttttc acattttaag gtttgtggta
     3601 aagcaaactg gaagtatata ccacaagtta tagagctttt agaaaaagca gaaaaggaag
     3661 gaataagagt atcttttgac caatatccat atgcagcagg aagtacaatg cttggagtag
     3721 ttttacctcc ttgggcacat tctggaggaa ctgataagtt aatagaaaga ttaagcgatg
     3781 aaaacgaaag agcaaaaatg aaaaaagata tagcaaatgg aatagaaggc tgggataatt
     3841 ttatagaatt tgctggaata gaccaaatat ttgtaacaag cgtaaaaaca gaaaagaata
     3901 aagatacaat tggtaagagc ttattagaaa ttggaaaaat gagaggaaaa gacccattag
     3961 atgcaacgtt tgatttatta aaagaagaag aaaatgcagt tgggatggta gatttctatg
     4021 gattagaaga acatataata ggattcatga aaagagacga gcaaaatgtg tgtacagatg
     4081 gtcttttagc aggtaaacca catccaagag cttatggttc tttccctaaa atactaggaa
     4141 gatatgttag agagttgaat gtactaacaa ttgaagaagc tgtttataaa atgactaaaa
     4201 aggcagctac aagctttagt ataaaagata gaggcgaatt aaaagaagga tattttgcag
     4261 atatagttat atttgacaag gatacagtta gtggttgtga tgattttatc aactctatgc
     4321 agtaccctac aggtatagat tacgtaatta taaatggaaa ttgtgttgtt gaagaaggaa
     4381 aatacaataa aattaaagca ggaaaagtac ttaaaaatta aatcttagct ttaaggagga
     4441 ataaatttat gattttagtg gggaatggga ggctaataag ccaagattct ttaaatccct
     4501 atatggaaga tggatgtgta gtaataaatg ataatctaat tgaagatata ggtactacag
     4561 aagaaatgaa agctaaatat ccagtacatg aatttataga tgcaggcaag aaaataatca
     4621 tgccgggtct aataaattca catatgcata tatacagctc atttgctaga ggaatggcag
     4681 ttccaggaaa accttctgag aactttttag agatactaaa taatttatgg tggagacttg
     4741 acaaacagct tacactggaa gatacaaaat acagtgcata tgcaacgtat atagaatgca
     4801 taaaaaatgg tgtaacaaca gtttatgacc atcatgcaag tccaaatgca attgaaggca
     4861 gtttgtttac aatatcagat gttgcaaagg atttgggaat tagaacgtgt ctttgttatg
     4921 aagtgtctga tagagatggt ggaaatacaa tccaacaagg tatcaatgaa aacgtaaact
     4981 ttataaaata cgctcaaaaa gatgatacag acatgataaa aggaatgttt gggcttcatg
     5041 ctgcatttac actatctgat gatacattaa ataaatgtgc ttcagagatg gctggtcttg
     5101 atacaggtta ccatgtacat gttgctgaag gactagcaga cttacaatat aatgtagata
     5161 aatatggaaa aagggtttta gaaagattca atgattttgg agtactaaaa gaaaaaacaa
     5221 tagcagttca ttgtatatat ataagtgcag aagaaatcag tcttttaaat aaaacaaata
     5281 catttgtagt tcataatcca gaatctaata tgggaaatgc agttggatgt tctcctgcaa
     5341 tagagttatt aaaaagggga gttacagtag gattaggaac tgatggatat actagcgata
     5401 tgtttgaatc attaaaagtt gccaatataa ttcacaaaca tcacttaaaa gacccaagag
     5461 ttggatttga agaatcacaa acaatgttat ttgataacaa tagaaaaata gctaaagctt
     5521 attataaaaa tgacctagga gtacttaaaa aaggtgctta tgcagatgtt atagtagttg
     5581 attatatacc acatactcca ttgacagaaa atacaatagg gggacacact atttttggaa
     5641 tgagtggaag aagcgtagat acaactatta taaatggaaa agtacttatg aaagatagaa
     5701 agttaataaa catagatgaa gaaagtattc tagcaaaaag tagagaatta agtcaaaatc
     5761 tatggaatag aatttaaata attgattatg caaaattcta tgaagcagga accctgtgac
     5821 tttagtcatg ggaggttcag attaatatac aagtaagaaa caagaattta gatgttaaaa
     5881 acagagctta aacaaaaata ggaatttaaa tgaagtatat aaataaaaaa tatacggaat
     5941 agaatattaa attaaatata gaagggagac ccaaaatgtc tcaagataaa agccctaaaa
     6001 tagctccagt agatgaaagg ttacctgtat ccaaatcttg ggtattttca ctacagcacg
     6061 ttttagcaat gtgtgcagga gcagtagcag ttcctatgat gataggaagt gctgcacgac
     6121 tagatcatgc tagtattgtt tttttaataa atgcttgctt gtttatggca ggtataggaa
     6181 cactcataca aagtgttggt ataggaaata ttgtaggtgc taaaatacca gtaatagaag
     6241 gaactagttt tgccgctgtg gcaggtatga cagcaatagc ctcatcatat agtgattcac
     6301 aagttgcaat acagacagta tttggctctg taatagtagc tggattgttt tgtatattac
     6361 ttgctccagt atttgaaaaa ttaataagat ttttccctag tgtagttact ggaacagttg
     6421 ttatggtaat aggtatttca ctatttccag ttgggataaa atggataact gacaacaaat
     6481 tggaagcggt tgaaccaaac tcattagcat tagctttagc agtattggca ataactttac
     6541 tttcatttaa attcttaaaa ggaatttgga atagcacagc tatattggta ggtatagtac
     6601 ttggtacact actttccatg gcagttggaa tggcagattt tagtgcagta aatggagttg
     6661 gttggcttaa cttaaatgta cctttaaaat ttggaatgcc tagatttgaa ccatctgcaa
     6721 tactatcatt gtgtttgatt atgttggtac ttatgacgga atcagttggt aatatgatag
     6781 ctattcatga aatggtagac aaagaagtaa caggtaaaaa tattaaaaga ggtctatttg
     6841 gagatggaat atctacagca ttatctggaa tattcaattc atttccaata actccatttg
     6901 gtcaaaatac aggaatagta ggtcttacag gtataaaatc aagatttata gcagtttacg
     6961 caggggttat attgctagta cttggattct taccaaaatt tgcagcaata ataggagcaa
     7021 tacctaagcc tgttctcgga ggcgtaggat ttgcaatgtt tggaatggta atggttggag
     7081 gagtaaaaac tttaagcaaa gtgaaatttg atggaactaa aaatggggtt atagtagcag
     7141 ttagtgttgg tttgtctatg ataccactaa caaatcccga tttctatcat aatttcccaa
     7201 cttgggtaca aacaatattc catagtggaa taacaacagg tagtttatca gcagtgcttt
     7261 taaacatatt cttcaatgaa ctaggttcta aaaaagaaaa aaataataaa gttgaagaag
     7321 caaaataaaa ataaaacaag taaaaatcta tattgactta aagtcattaa gcattaataa
     7381 aaataattca gagatattaa taattcaaca aaactatctt atattttaaa taatataaaa
     7441 aagtaaataa ataacgaata caaataaaag aatacaatct aaatattaaa aatatttaat
     7501 ttaagggaga gataataata tggctaattt aaaagttaat ttatatggaa tggaattttt
     7561 aaacccaatt atgacagcgg caggacctgg agcaaaagat ggtgctctct gtcaagaggc
     7621 agcaaaaggt ggagcaggag ctatatgtac aaaaactata tctgtattac cagctgatgt
     7681 accaagacca tgtatggcaa aaactaatag tggtttttta aacacagaat tatggtcaga
     7741 gcttccaaaa gagcaatgga tagaggaaga atacaaaaag gtaaaagaaa cagggcttcc
     7801 agtaatagta agtatgggat attcagcaga gcaaatagct gaggttgctc ctttagtaaa
     7861 gccttatgca gatgcagttg aattatctac tcattatgta ggtacagacg ttgcaccaat
     7921 agtaaatgct ttaaaagctg caaaagctgt tttagatgta ccagtattta tgaaaatgag
     7981 tcctcatact gacattcaaa ctattgcaaa agctgttgaa gaagcaggtg cagatggact
     8041 tgtaatgata aactcatttg gaccatgtat ggcaatagat gtaaatacag gataccctat
     8101 aatgggaagt aaagctggat acggatggtt atcaggtgca ccaataagac ctctagcaat
     8161 aagatgtata tatgaagctt ctcaagttgt aaatatacca ataatcggtg ttggtggaat
     8221 cacttgtgga aaagacgtgg ctgaaatgat gatggctgga gcaagtgctg ttcaagtatg
     8281 tacagaagca atccttaaag ggccttctat ctatggaaaa attgctaaag aattaaatga
     8341 atttttagat gagaatggat atgctgatgt taatgaaata aaaggattaa ctcataagaa
     8401 aatggcagct agagggttta gaactcattc agtagctcca gtagtggatg ataacaagtg
     8461 catcaaatgt aagcaatgtg taacaagctg tgtatacgaa gctttagaag ttacagataa
     8521 gttgaacata gatgcagaca agtgttttgg atgtggatta tgtgtaacta gatgtccaaa
     8581 aggagcttta agcatctgct acaatcaagt agaagctatg aatgagtaat aattatatag
     8641 aattttggta attaacttag agtaataggt attaggataa taaaatatat cagataaaca
     8701 aacacacatt atgcattaat aatacagtct aaagttaata attttattat attgatttaa
     8761 ctaaatatta gtttagctga atattaattt aattaggtat tggtttaatc ttaatataga
     8821 tttactacgt ataagtgaac taactagatt actttaagtt aattttttca tataaaataa
     8881 attgatacat tacaagtaag gtggagtcta tatgggaatt gttttaaaag gtgggaaagt
     8941 agtaacaagt gaaaaaactt actgtgcaga tgtaagaata gaagatgaaa aaatagtcga
     9001 tataggtctt gatatatcaa aagaaaatga tgaaatagtt gatgttagtg attcttatgt
     9061 tataccagga ggtatagata cacacacaca ctttgactta gatacaggcg ttactaagac
     9121 agcagataac tttgagacag gaacaaaagc tgctatagta ggaggaacta ctactatatt
     9181 ggattttgct acacaagcta aaggatgttc tttgaaagac ggattagaag aatggcatga
     9241 aaaagctaag gataaatcct attcagatta tggctttcat atggctataa cagattggaa
     9301 tgactcggtt tgtaatgaaa tggaggatat ggtgaaagag ggagtatctt catttaaact
     9361 gtatatggcg tataaaggaa gccttcaagt tgatgatggt gtaatctttg aagcattgag
     9421 aaaagcagaa gagataggag ggattattgg tttccattgt gaaaatgggg acataatatg
     9481 tgaacttgtg gataaagcaa agtcagaaaa taatttatct ccaaaatatc atcaattaac
     9541 tagaccagct ataatggagg cagaagctac atcaagactt atgaagatag ctgaagttgc
     9601 aaactcaaaa gtatatgtag ttcacttaag ctgtaaagaa tccttagagg aggttttaaa
     9661 agctaagcaa agaggtgtgg atgtaatagt tgaaacttgt cctcaatacc ttctattaaa
     9721 tgatgaatta tatgatttag atggatttga aagtgctaag tatgtaatgt caccaccttt
     9781 aagaaataaa gaaaataatg aaatattatg gaatgctatt ttagataatc aaatagacac
     9841 tataggaaca gaccattgtt cttttaatta taagggacag aaggaacttg ggataaacga
     9901 tttcagtaag ataccaaatg gttctccagg tgtagaacat aggatgagtc ttttatacac
     9961 atatggagtc tgtaaaaata aaatatctat caacaagatg gtagaagtaa catcaactaa
    10021 tgcagcaaag ttgtttggaa tgtatcctaa taaaggaact atagaaatag gcagtgatgc
    10081 agatatatta gtcttaaatc caaacaaaaa atccaaaata gaagcaaaaa aacaattaca
    10141 aaatgtagat tatacacctt atgaagggta tgaagtggat tgtcaaatag aacatgttta
    10201 tttaagagga aaagaagtag ttagagatgg agaattaatt tgtaatactc caagtgggag
    10261 atatataaat aggcgtctaa caaagggggc tagagaatgg tagaatttat attaaatgga
    10321 gagaaagttg ttgcaaaaga aggcaaaaaa cttttagact tccttagaga agatatgaat
    10381 ataacttcag ttaaaaatgg ctgttcagaa ggtgcttgtg gaacatgtat ggttataata
    10441 gatggtaaag ctactaaagc atgtgtattt aaaactgata aattagctgg gaaaaacata
    10501 acaactatag agggattatc agagagagaa agagatgttt ttgcatattc ttttgctagt
    10561 caaggagctg tacaatgtgg attttgtatt ccaggtatgg taattagttc aaaagcactt
    10621 atagatgtta ataataatcc tactgaagat gatataaaga aagctttaaa aggtaatata
    10681 tgtagatgta cagggtatgt aaaaataatt aaagctgtcg atttagcagc aaagattttt
    10741 agagaaaata tagatgtgcc aaaagtaaac tgtaagggtc tagtaggtga aaatcttcct
    10801 agagttgatg cagttggcaa agcacttgga tattctgaat atgtagatga tatgagaatt
    10861 gagggtatga tttatggttc agcagttaga gctaagtatc ctagagcttt agttaaaaaa
    10921 atagatatag aagaagcaaa agcttatcca ggagttgttg cagttctaac agctaaagat
    10981 attccaggaa ctataaaggt agggcactta aagaaggatt gggatacttt aataccagaa
    11041 ggtgaaataa ctagatattt aggagatgga atagttttag tagcagcaga aactagagaa
    11101 gcgttagaag aggccaaaaa gctagttaag atagattatg aggaattaac accattaagt
    11161 actacagcag atgcacttag agaagatgct ccaaagatac atgagacagg gaatatactt
    11221 gttaaggaac atttagtaag aggaaacgct aaagaaaaga tagaaaattc aaaatatgtt
    11281 gtaactaaaa gatatactac acctgctaca gaacacgcat ttttagagcc tgaatgtgca
    11341 gtagctggtc cttatgaaga tgatggagta ataatctatt caacagacca aggggtgttt
    11401 gccactcaac atgaatgtgc agatatgtta gggttaccat atgagaaggt aagagttata
    11461 aacaaaatgg taggaggagg gtttggtgga aaagaagaca tgagtgttca acaccatgca
    11521 gccttacttg cataccatac aaagagattt gtaaaagtac agttaacaag aaaagaaagt
    11581 attattgttc atccaaaacg acatgccaca gaaatggaat ttacgacagc atgtgatgaa
    11641 aatggatact taacgggtat gaaggcaacg ataatatctg acacaggtgc atatgcctct
    11701 cttggaggac cagtgctaca aagactgtgt actcatgcag caggaccata caattatcaa
    11761 gatattgata taacaggaac agcagtatat actaataacc ctcctggagg agcatttaga
    11821 ggatttggag ttacacagag ttgttttgca actgaatgta atctaaattt acttgctgaa
    11881 atggttggaa tatctccttg ggagataaga tataaaaatg caataagacc tgggcaagtt
    11941 ttaccaaatg gtcaatttgc agatgaagga acagcaatgg cagagacact agaagctgta
    12001 aaagaagagt atgaaaagca tccacgtgca ggtatagctt gtgctatgaa aaatagtggg
    12061 gttggagttg gtatacctga tgttggaaga tgtagattgg tgataaaaga tggcaaagtt
    12121 cacataagaa caagtgcttc ttgtataggt caagggcttg gtactgttat gactcaagta
    12181 gtttgtgaaa ctgctagcct acttcctgaa aatatagtgc atgaagttcc agatactcat
    12241 ttaacaccag actctggtaa tactacggct tcaagacaga cagtatttac gggagaagct
    12301 actaaggttg catcaaataa gttaaaagaa gctttagact ctggcaaaac tttacttgat
    12361 ttagagggag aagagtttta tggagaatat gaaagtatta cagataaaat gggttgtgac
    12421 aaagaaaatc cagtaagtca tgttgcttat ggatatgcaa cacaagttgt aattctggat
    12481 gaaaatggaa aactagaaaa agtagtagca gcacatgatg ttggaagagc tataaatcca
    12541 atcagtttag aaggtcaaat agaaggtgga gtagttatgg gtcttggata tgctctaacc
    12601 gaagattacc cagtagttaa ctctgttcct acagctaagt ttggtacatt aggtttattt
    12661 aaatctacag atataccaga aatacaagct ttaatagttg aaaagaacaa agatttattg
    12721 gcttatgggg caaaaggtgt aggagaaatt tgtacagttc ctactgcacc agcagtacaa
    12781 aatgcctatt atgtatttga taaagaattt agaactgagc taccactaca aaatactcca
    12841 tatagtaaaa aaaagtaaca aaaaaaagaa atattaaata aaaaaataga tgaaattgta
    12901 aataaaaaaa acccttttgc ataaggtata ttttaaatga tagaatatac atatacagga
    12961 gggtttttct tttataaaat aagtttaata aaattgagag gtgagcagaa tgaattttaa
    13021 taatagtcta gaattaactc agtctcagaa gctaataatg actacacaac ttaagcaatc
    13081 attatcaata ttaaatatga gtaagttaga gttagaagaa gagataaaaa gagaagcaga
    13141 gaataaccca ttagttgaag tcgagaaaag tggggagata aattgggaag agtatataaa
    13201 agacatggat aggtcaagac gtttagatag aacagagata agctataatc ctgataatga
    13261 agttaatcta gaaaatttag ttaaatattc gtcaaattta tatgaagatt taaaatttca
    13321 gataagcctg tataaactta cagacaaaga attggaagtc tgtgagtata taattgatag
    13381 tttagatgag gatggatatt taagaactga ggaaaaagta attgttgata ccttaaagat
    13441 agatgaagaa ctttttgaaa aatgtttaat tagtgttcag cagttggaac ctagtggagt
    13501 aggggcaaga agtctttcag aatgtctgat gatacaaatg gctagtttag gaatatataa
    13561 tgaaatttta gaggaaatcg ttacaaaaga cttaaattta attgcaaata ataaatataa
    13621 agagataagt aaaaagtaca atatgtcatt acaaaaatgt gttgaactaa taaacataat
    13681 taagacatta gaccccaaac caggaagaac ttgttcagta gagaaaagtg tatacattca
    13741 acctgatgta acagtagaaa agattgatga tgaatttatt gtatatttaa atgagaaaga
    13801 ttcttaccaa ataagaatca atagttatta taaagacata ttaaaaaatt cacagtctga
    13861 tgaaagtgca aaagaattta taaaagaaag actaaattca gcaacaggac ttatgaaaaa
    13921 tatagagagt aggaaaacta cagtacttaa gattgcagaa gagatagtaa aagctcaaga
    13981 tgaatttttg agaaaaggga caaaatatat aaaacctttg aaaatgaaag acatagcaga
    14041 aaaattagaa tttcatgaat ctactataag tagaggtgta aatggtaaat atatgctaac
    14101 accttttggc gtatatgaat ttaggtactt tttcagtagc gccattgaga ctgagaataa
    14161 tgaaatggca tctagtacaa gcataaaaaa aattataaag gaaacaataa aagacgaaaa
    14221 taaaaagaaa cctttaagtg atgaccatat atctaagtta ttaaaagaaa aaggaatcaa
    14281 tgttgcaaga aggacagttg ccaaatatcg tgaagaatta ggaatcccat cttcaagtaa
    14341 gagaaaagaa tactaataag taaaagacaa atatcagtat ggtaagggga aaaggctaat
    14401 taagagatga atgtagattg ggctaaaaat tttataaagt aaaataatag aatatggaaa
    14461 agtattaaaa tgctataagg tattaaaaaa ctggggaaca ttaaaacatt aagatagcaa
    14521 agaacataaa aaactaaaga agattaaaac attaaagaac attaggcttt gaggaatatt
    14581 agaactttaa agaatattaa aatttataaa atataaaaat actaaaaaga attgaaaaac
    14641 tggggaacat taaaatattg taaaatgcta aaaaactaga aaacataatt aaataagtgc
    14701 ttataaagaa tgattaaatt aaataagtgt ttgtaaagaa taattagata ttagaaaaag
    14761 aagaatacct agataaataa actattatta aatttaataa tcacaaaagg aaaacacgta
    14821 gggtataaat aaaaatctta actagataaa agtaaactta gttgtagata aaaataacta
    14881 gaaataaaag taactagaaa taaaattatt tatagtaata aatattagaa tgaaaatata
    14941 aaaaaatata tataaaaagc cttatataaa aagaaaaaat actgtgattt gaaaatggtg
    15001 gagaaatatt ttataaaata aaaaaataaa aaagtttttg aaaaactatt gaaatctata
    15061 cgcaaatcct ataaaataaa aatatcttgt gggacttaaa taaacactag ggacaaaaaa
    15121 agtcccgaaa ggaaagcaat aaggagtatg ttatgaagaa cttattagaa attcaaaaga
    15181 aattgattcc acaggcaatt gaacttatgg aaagaagata ctctatttta agacaaatat
    15241 ctttaagtga accaataggt aggagaacat tatcaaatgt attagagatt agtgaaagaa
    15301 taatcaggtc agaaactgag tttttaaaag aacaaggact tatagacgta gctgtatctg
    15361 ggatgactat cactaaagaa ggttctgatt tgttagataa acttaaggac atcatgagtg
    15421 acatcatggg attgtctaag ttacaagata gagtcagaga aaagcttgga ataaaaaggg
    15481 taatactagt tcctggaagc tgtgatgaaa atggaagttt actaaaagat gtaactagat
    15541 atggatgcga gtatttctta ggcattttaa aagatggaga tatcgtttca ataacaggag
    15601 gaagcactat gttggagttt tcaaacgtta ttaaaactga taagagatat ccaaactcta
    15661 caatagttcc agctagagga agtatgggaa ctgatgtaga gatgcaatct aacaatatag
    15721 ttgcttcaat aagtaagaaa ttacattcta attgcaaact tttacatata ccagatgagt
    15781 tggatgaaag tgcaatgaaa actcttagcc aagtgccaga gattaagaca actttggagt
    15841 atattaaaag aacagacatt ttagttttta gtataggtag agcagatgtc atggcaaaga
    15901 gaagaaaact tccagaagac aaggttgatg agatattatc taaaaatgca gttggagaag
    15961 catttggata ttatttcaac aaagatggag aaatagttta caaactgaac actgttggaa
    16021 ttgatttaga aacagttaaa caagtaaaag aaactattgc aatctttgca gggactgaaa
    16081 aagtagaagc cttattagct atatcaaata tgcataaaaa catggttctt gtaacagatg
    16141 aagaaagtgc ttataagata ttgtcattat tataaaagtc aagacctagc acagagctag
    16201 aaaacataca tttactttat aggaggtagc gaaaatggtt aaagtagcta taaatggatt
    16261 tggaagaata ggaagattag cattaagaaa aatgatggag caacaagata agttcgaagt
    16321 tgttgcaata aacgacttaa cagacgctaa aatgttagct catttattta aatatgatac
    16381 agctcaagga agatttaacg gagaaataga agttaaagaa ggtgcttttg tagtaaacgg
    16441 aaaagaaata aaagtaacag cagaaagaaa tcctgctgac ctaccatggg ctgaattagg
    16501 agtagatata gtattagaat gtacaggatt ctttacatct aaagataaag ctgaagctca
    16561 tatacaagca ggggctaaga aagttgttat atctgcacca gctactggag atttaaaaac
    16621 aatagtattc aacacaaaca gtgacatatt agatggttct gaaacagtta tatcaggagc
    16681 ttcttgtact actaactgtt tagctccaat ggctaaagta ttaaatgata aatacggtat
    16741 agaaaaagga ttaatgacta ctatacatgc atacactaat gaccaaaata ctttagatgg
    16801 tcctcatcca aaaggtgatt taagaagagc tagagctgct gctggaaaca tagttccaaa
    16861 cactacagga gctgcaaaag caataggctt agtaatacct tcattaaaag gaaaattaga
    16921 tggggcagca caaagagtac cagttgtaac tggttcaata actgaattag tttgtacatt
    16981 aggaaaaaat gttacagttg aagaaataaa tgcagctatg aaagaagctt caaacgaatc
    17041 tttcggatat actgaagaaa tgttagtatc ttctgacata atcggaataa gctacggttc
    17101 attatttgac gctactcaaa caaaagttat ggaagtagat ggaaaacaat tagttaaagt
    17161 tgtttcttgg tatgataatg aaatgtctta tacttctcaa ttaataagaa ctttaggata
    17221 ctttgcacaa ttagcaaaat agtcagtttt gagaaaatag aaaagtagat tgacaaagtc
    17281 cgagtttgta gtttaatact acggactcag gacttttttt aagaaaaaat atataaaata
    17341 cacctaaata atgttacata ctatatataa aaatatgata atatgatata tttaataggg
    17401 taaaagtatg tactatatta tacttggcaa taaaaaatta ctttattaaa aggagagata
    17461 tttatgtcaa tgcttaacaa aaaaactata gaagatattg atgtgtgcgg aaaaaaagta
    17521 ttagtaagat gtgactttaa tgtgccatta caagatggtg taattactga tgaaaataga
    17581 ttgaacggag ctttaccaac tattcaatat ttaatttcta aaggggcaaa agttatatta
    17641 tgctctcact taggaaagcc aaaaggagaa gctaaaccag aattatcttt agcaccagta
    17701 gcaaagagat tatctgagat gttaggaaaa gaagttgtat ttgcagcaga cgataatgtt
    17761 gtaggagaaa atgcaaagaa agcaactgaa aaaatggaaa acggagatgt agtattatta
    17821 gaaaatacta gatatagaaa agaagaaact aaaaacgaag agaatttctc taaagaacta
    17881 gcatcacttg ctgaaatatt tgtaaatgat gcatttggaa ctgctcatag agcacattgc
    17941 tcaacagttg gtgcaggcga gtttttacaa gagagagttt gtggatactt aattcaaaaa
    18001 gaattaaaat tcttaggaga agctgttgca aaccctgtaa gaccatttac agctatactt
    18061 ggaggagcta aagtttctga taaattagct gttataaacg aattattaga aaaagttgat
    18121 aatctaataa taggtggagg aatggcttat acattcttaa aagctcaggg ttatgaagta
    18181 ggaacttctt tacttgaaat agataaagtt gaatatgcta aagaaatgat ggaaaaagct
    18241 aataataagg gtgttaactt attattacca gtagacgttg ttatggcaga ccactttgca
    18301 ccagatgcta ctccaatagt aacagaagat gctaatgtaa aagaagatta catgggacta
    18361 gatatgggac ctaagactat agcaaacttt gtaaaaacta taaaagaatc aaaaactgta
    18421 gtatggaatg ggccaatggg agtatttgaa tttgaaaact ttgcaaatgg tacattatca
    18481 gtagctagag ctatggcaga gttgactgat gcaactactg taattggcgg tggagatagt
    18541 gcagcagcag ttaatcaatt aggatttggt gacaagatga ctcacgtatc aacaggtgga
    18601 ggagcatcac tagaattttt agaaggtaaa gaattaccag gtattgcagc attagataat
    18661 aaataaacta atatatagag gagattgtaa aatgagaaaa cctataattg caggaaactg
    18721 gaaaatgcat aaaacaataa aagaagcttt agaatttgta aatgaagtta aagataaagt
    18781 aaattctgat aaagtagaag ctgttatatg tgcaccattt actttattaa aggatttaaa
    18841 agaagctact aagggtacaa atataaagat aggtgctcaa aatatgcact ttgaagaaaa
    18901 aggagcattt acaggagaag tttcacctct tatgttaaaa gaaatagata tggactatgt
    18961 tgtaatagga cattcagaaa gaagacaata cttcaatgaa actgatgaaa ctgtaaacaa
    19021 gaaagtttta aaagcattag aagtaggaat agacccaata ttatgtgttg gagaaacttt
    19081 agaacaaaga gaagctggaa aaactaaaga tgtttgtaaa gttcaagttg aaaaagcttt
    19141 agaaaatgtt cttaaagatg atttatcaaa agtagttgtt gcatatgagc caatctgggc
    19201 tattggaact ggtaaaactg caactgctga agatgctaat gacgttatat cttatataag
    19261 agaagttata aaaggattat atggagaatt agctaatgaa gttagaatac aatacggtgg
    19321 aagtgttaaa ccttcaaatg tagccgaaat aatgggtcaa agtgatatag atggagcttt
    19381 agtaggtgga gctagtttag ctagtaatga ttacctagac cttgttaatt tctaaggagt
    19441 gaaatgatga aaaaaccagt tgctttaatt attatggacg ggtttggata caataaggat
    19501 gttaagggaa atgcaattgc tgagtccaaa actcctaatt tagatagaat aaaaaaagag
    19561 tatccaaaca cattgataaa tgctagtggt cttgatgttg gtctacctga tggtcaaatg
    19621 ggaaattcag aagttggaca cactaacata ggtgcaggta gaatagtata tcaagattta
    19681 actagaataa caaaatctat aaaagatggt gacttttttg ctaataaagt tttatgtgaa
    19741 gcaatggata atgcaaaaga aaattctctt catgtaatgg gacttttgtc tgatggagga
    19801 gtacattcac atatagacca tcttaaggct ataatcaaaa tggcaaaaga taaaggcgta
    19861 caaaaggtgt atgttcacgc atttactgat ggtagagata ctgaccctca aagtgcatta
    19921 gaatatgcca aagaagtaca agctagtatg gatgaaattg gagtaggtga atttgctaca
    19981 gtttctggta gatactacgc tatggataga gataagagat gggaaagagt agaacttgca
    20041 tacaatgcca tggttagagg tattggtgaa aaagcaaatt ctattgagga agctattcaa
    20101 aactcttatg atgatggaaa aaatgatgag tttataatgc caacagttat tatgaaagat
    20161 gataaaccag ttggaagtat aaaggaaaat gattcaatta tattcttcaa ctttagacca
    20221 gatagagcta gacaaataac tagagcttta gtttgtgaag agtttgatgg atttaaaaga
    20281 gaagatatta aaaacttctt tgtatgctta acagagtacg atataactat agagaatgtg
    20341 catatagcat ttggacctca atctttagcc aacactttag gtgagtatct agctaaaaat
    20401 ggaaaaactc aacttagagc tgctgaaact gaaaagtatg cacatgtaac tttcttcttc
    20461 aatggtggag tagaagagcc aaataaaggt gaagaaagac tattgatacc ttctccaaag
    20521 gttgcaactt atgatttaaa accagaaatg tcagcatatg aattgacaga taaagcttta
    20581 gataaattag gagaagataa gtttgacttt atagtattaa actttgcaaa tcctgatatg
    20641 gtaggtcaca caggaagtat agaagcggct ataaaggctg ttgaaactgt agatacttgt
    20701 gtaggaaaat tgatagataa aatagtagag ttaggtggaa gtgcaataat aactgctgac
    20761 cacggaaatg ctgaatatat gttagacccg gaaacaggaa aaactgtaac ggctcactct
    20821 attaatccag taccatttat tgtagttggt caagaatatg aaagtgcaaa attattggat
    20881 ggaggaagat tatctgatat agcaccgact gttttagata tgatgaagtt agaaaagcca
    20941 gaagaaatga ctggtcattc acttatttca aaatagtcga ttctgataag tagagaataa
    21001 tatataaact acaatataaa tttaaaaata atgcagttta ggtttatttt gtaaagttta
    21061 gactttatga cataagattt agattttgta agttctggat attgtaaggt gtagatgaag
    21121 gtatatttta taaaatttac aagatagaca atttataaaa taaaaaatta taatatagat
    21181 aataatgtaa ataatgatat aaataataac aagatagaca ataacaatat aaataataat
    21241 aattaatata tattatataa gcaaaaattt tagaatcatt a
//
LOCUS       DAEPOZ010000041        21036 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.041, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000041 DAEPOZ010000000
VERSION     DAEPOZ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21036
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.041"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            105..416
                     /locus_tag="KR440_001213"
     CDS             105..416
                     /locus_tag="KR440_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698271.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VRKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            418..2343
                     /locus_tag="KR440_001214"
     CDS             418..2343
                     /locus_tag="KR440_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBH0698272.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPLENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFDCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            2346..2837
                     /locus_tag="KR440_001215"
     CDS             2346..2837
                     /locus_tag="KR440_001215"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBH0698273.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            2889..3452
                     /locus_tag="KR440_001216"
     CDS             2889..3452
                     /locus_tag="KR440_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HBH0698274.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            3467..4444
                     /locus_tag="KR440_001217"
     CDS             3467..4444
                     /locus_tag="KR440_001217"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HBH0698275.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            4431..4754
                     /locus_tag="KR440_001218"
     CDS             4431..4754
                     /locus_tag="KR440_001218"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBH0698276.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            4871..6649
                     /locus_tag="KR440_001219"
     CDS             4871..6649
                     /locus_tag="KR440_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBH0698277.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            6646..8019
                     /locus_tag="KR440_001220"
     CDS             6646..8019
                     /locus_tag="KR440_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBH0698278.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            8025..8693
                     /locus_tag="KR440_001221"
     CDS             8025..8693
                     /locus_tag="KR440_001221"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBH0698279.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            8970..10019
                     /locus_tag="KR440_001222"
     CDS             8970..10019
                     /locus_tag="KR440_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH0698280.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTYNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            10115..11104
                     /locus_tag="KR440_001223"
     CDS             10115..11104
                     /locus_tag="KR440_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0698281.1"
                     /translation="MSYLKINNVFKSYDQKRVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKEKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDVSIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            11109..12827
                     /locus_tag="KR440_001224"
     CDS             11109..12827
                     /locus_tag="KR440_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBH0698282.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAAALAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(12917..14062)
                     /locus_tag="KR440_001225"
     CDS             complement(12917..14062)
                     /locus_tag="KR440_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH0698283.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQDVINKKYIVYRIGGDEYIIILKNLSHKEINSIINNINLKIV
                     EENSTNELKLSMSIGYAIFDEKVDVSLFDTYRRADSQMYDNKKKINK"
     gene            14561..15922
                     /locus_tag="KR440_001226"
     CDS             14561..15922
                     /locus_tag="KR440_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0698284.1"
                     /translation="MKDRINLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAIYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     MSE"
     gene            16017..16721
                     /locus_tag="KR440_001227"
     CDS             16017..16721
                     /locus_tag="KR440_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0698285.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            16921..17232
                     /locus_tag="KR440_001228"
     CDS             16921..17232
                     /locus_tag="KR440_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0698286.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            17254..18567
                     /locus_tag="KR440_001229"
     CDS             17254..18567
                     /locus_tag="KR440_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0698287.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            18644..19954
                     /locus_tag="KR440_001230"
     CDS             18644..19954
                     /locus_tag="KR440_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBH0698288.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNNGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLVAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            19980..20711
                     /gene="chbG"
                     /locus_tag="KR440_001231"
     CDS             19980..20711
                     /gene="chbG"
                     /locus_tag="KR440_001231"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBH0698289.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLVSLKPVVEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            20724..>21036
                     /locus_tag="KR440_001232"
     CDS             20724..>21036
                     /locus_tag="KR440_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBH0698290.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ES"
BASE COUNT         7903 a         2564 c         3912 g         6657 t
ORIGIN      
        1 tataaaaatg ctgtttatac tttaacaatg aggtagtaat ctaattagaa taaaaaaatc
       61 taattggtta tatacatagt aaaaaggtaa gagaggtgat ttggatggct tttgaaataa
      121 taaaaagcat tgttgaggca gagcagacag cagacagtat caaagtaaaa gctgttactg
      181 atgcagagtc tatcagagct gatgctgtaa acaaatgtga aagcatattt gctgatgtaa
      241 gaaaacaagc aaagcttatg gaagaaactc ttattgagaa ggcagtcacc gacagtagag
      301 cagaggttga taaaatctta gctaatgcta aaagtgaatg tctgaaaatt gaaaaaactg
      361 ctgaagaaag aaaaagtaag gctattgaag cggttattgg aaaggtagtg agataatatg
      421 gcaatagtta atatgaacaa aatatctatt gttggcttag aagctcaaaa aagccagatt
      481 ttaaagctac ttatgaaccg tgggtttgtt cagattgatg atagtgcctt tcttactgag
      541 gaggaggaat taaaaaatta tcttgtaaaa gatagtgaag aatctgaagt tataatgcta
      601 gagcagaaaa tgtctcatgt aaaccaagca attaatattg tttcttcact agtgaaacaa
      661 aaaaaatccc tacttgcacc aaaaagagag tttaacaaaa ttgatagtga aaaagaaggg
      721 caagtttatg ctgaagctct ggagttaaac aatttgcaca aggagatagg tacaataagg
      781 tctaacataa acttacttaa caacaataaa agtatgttag aaccatgggt gaactttgat
      841 attcctcttg aaaatatgga aactaagtat acaaaaacta tacttggaac tgtgcccata
      901 agcgcaaata ttgatgcttt aagagtaaaa ttagaagatg aaataccaga aagtgttatt
      961 gggattgtca attctgacaa gttaatgaac tatgtttatt taattacttt aaaagaaact
     1021 tttgatgatg ttttagaagt tttaaaagaa tttaattttt cagtggtttc attaccaaat
     1081 gaagaaggta caccgttaca agctatcaag aaatacaatg aaatgataat taggcaggaa
     1141 aacgtttcta aggacaaaac agaggaaatt aaaagacatg cagatagtat acacatgctt
     1201 gaaaacctat atgattttta cactatagaa agagaccaaa aaaagattat agagagactt
     1261 gtcaaaacaa aaacaacatt ctgtcttaat ggttggttac caagcaaaag ggcagatgaa
     1321 ttaattaaag aaattacaga aaagtttgat tgttgtgttc agactgaaga aggaagtaaa
     1381 gaagaaggct tcccaattct gcttgaaaac aataaattag taacaccttt tgaaagtgta
     1441 actaacatgt acagctatcc aagtacaaaa gatattgacc caaatacaat tttaactttc
     1501 ttctttgttg tattctttgg aatgatgcta agtgacgccg catatgggtt aataattgca
     1561 gtagtctgtg gatttgttgt atataaatta aagatacaaa aaggtgaagg aaatttaatt
     1621 aaacttattg gtatctgtgg agtttcaact actgtatggg gtcttatatt cgggggtatt
     1681 ttgggggatt taattcctat aaaagcatta attaatcctt tagaggatgt tatgacactt
     1741 atgggaatgt cgttactgtt cggtattatc catatatatg taggtttggg aataaaagca
     1801 tatacattaa taagaagtgg aaaggtaata gatgcaatct ttgacatagg tttttggtat
     1861 ttgtgtataa caggtgtatg tctacttata ataccttttg ttgcagggga cataggtgta
     1921 ttttcagaag taggaaaata tttagcaatt ataggtgcta taggtcttgt acttactcaa
     1981 ggaagaagct ttaaaggtat tcctgttaag ctttttaaag gattttcaag tctctatggc
     2041 ataacaagtt attttgctga tatattatct tatacaagaa ttatggcgct ttgtttaaca
     2101 acaggagtta tagcacaagt tatcaacctt ttaggggcta tagctggtcc gatacttgca
     2161 gttgtaatcg gtgttgtagg acacacaata aaccttctta taaatgcact aggtgcatat
     2221 gttcatacaa gtaggttaca gtatgtagaa tttttcaata agttttatga aggcggagga
     2281 gttccttttg ttccgtttaa gtataaaaca aaatatacaa gcattaataa aaaggagatg
     2341 taaatatgga atttttaaac gcgttaggaa atggtcaatt tttggcaata tcaggagctg
     2401 ctatagcagc attatttgca ggtatgggtt ctgctaaagg tacttcaata gcaggtcaag
     2461 ccgcagcagg tgttgtaaca gaagacccta gtaaattcgg tcaacttcta gttttacaac
     2521 ttcttcctgg tacacaaggt ctgtatggat ttatcatagc tttccttatt ttatcaaaag
     2581 cagggattat tggtggagat gcaataccaa catcagctca aggtttacaa cttcttatgg
     2641 caggacttcc aattggttta gttggtttag tatcaggtat agcacaaggt aaagcagcag
     2701 cagcaggagt tggaatagtt gcaaaaagac ctgaagaact tggtaaagct ataacatttg
     2761 cagcaatagt tgaaacatat gctcttttag gactacttgt ttctttctta gcttacaatg
     2821 gaattgcaat tggttaatta taaattataa ttcataagta aaaaaaatga aaaggaggaa
     2881 gcactcctat gggtaatgaa caaaaaatga ttgacagaat tatagctgat gccaaacaag
     2941 aagcacaaga aatattggat aaagctaaaa gtgaagcaga tttaaaagta aattcagcta
     3001 atgaaaaagc tgaaaaggaa atggcttcct atacaaagct tgcagaggca gaagcagaaa
     3061 aagcggcttc taaggaaata tcaggagctt atatggaggc taaaaagcaa atattatcaa
     3121 aaaaacaaga gattttagaa gaggtaattt tagaagctaa aaacaaactt ttaaatctta
     3181 aagataatga atatgaagag attatactaa acatgattga aaaatcaaat tgtacagatg
     3241 attcagagat agtattatct aaaaaagata aaaaaacatt aaaagatgtg ctatcaaaaa
     3301 aaggcattaa agtgtctgat gaaactagag atattactgg aggtttcata gttaaaaaag
     3361 gtgacattga gtacaattat tcatttgaag ccataattgc agtagagcat gaatatattg
     3421 aacaaattgc agcagaaatt ttgtttaatt aaggaggggt taaaaaatgg cagaagaaaa
     3481 aacttatccc tatgccgttg cacgaataag agtgcttgaa aaacagcttc ttaacagaca
     3541 aatgtttatt caaatggcag aagccaaaag ccctgaagac tctttaagaa ttatagctga
     3601 ggcaggttat gctgatacat caaataataa tatacataat tttgagaata ttttgactca
     3661 agaattatca aagacatatg aaattcttaa aagtctagct cctgaagaaa aatttgttga
     3721 tgtatttctt tataaaaatg actatcacaa tttaaaagtg ctgattaaag aagaaatttc
     3781 tggtgtagat ggtgagaaat atttaattga tggaggtaca attcctctta ttaagcttag
     3841 agaatcttta gcaaatcgta gttttagtga tttacctaag ataatgtcat cagctatatt
     3901 agaggcattt gatgtttata ataagacaca aaatggccag atggttgata ttgttctaga
     3961 taaagcaacc tttaccagta tgaaagaaac tgctaaagaa agccaaaaca aatttgtaat
     4021 agattatgtt atgaaagttt gcgatttaac aaatctaaag agttttataa gagtcaaaaa
     4081 tatgaagaaa gattttgata tgtttatgaa tgtgtttgtt tcaggtggct cacttgataa
     4141 ggaaaaattc ttagaagctt tttcctcaga tactccagca tcatgtttta aatctacttc
     4201 ttactctgat gtctgtaaaa atggcatgga tagtggtttt actgtctttg aaaaactttg
     4261 tgatgactat cttatggaat atgttagagg agcaaaattt aagcctctta cattagaacc
     4321 actaattgca tatttatatg ctaaagaatc agagataaaa acaattagaa ttattttgac
     4381 tagtaagctt aataatatag acgcagatac tataaaagaa aggctaaggg atgcttatgt
     4441 ataaggtagg agtagttgga gataaagata gcatcatggg atttttagct cttggaatag
     4501 atatcttccc agcctatgat agtgacgaaa ttaaaaaaag tatacataag ttggttgaag
     4561 atgaatatgc gataatttat attacagaac aggcatcatt gcttgcaaaa gaatcaatag
     4621 caaaatacaa agattaccaa cttcctgcca ttatagttat tccaggtatt ggaggaagta
     4681 tgggattagg tatgaatgaa gtaagagaat ctagtaaacg tgctataggt gcagatatat
     4741 tatttaaaga atagatgaaa gttgaataaa agacaatctc aaattttatt ggtgctaaag
     4801 atatgtgcta ataaaatata ggagggaata tttaaatttt gataaagtat atgattgcag
     4861 gtgagagaat atgaaaacag gcacaattgt aaaggtgtca ggaccgcttg ttgtagcgga
     4921 gggcatgaga gatgcgaata tgtttgacgt cgtaagagtt tcagataaac atcttatagg
     4981 cgagattata gaaatgcatg gagataaagc ttctatacag gtttatgagg aaacttccgg
     5041 tctaggacca ggggaagaag tagtttctct tggaatgcct atgagtgttg aacttggacc
     5101 aggtcttatt tcaactattt atgatggtat tcaacgtcca cttgaaaaaa tgtatgatat
     5161 ttcaggtact aatataacta aaggtgttga agtatcttca cttgatagaa caactaagtg
     5221 ggaatttaaa cctaaaaaat ctgttggtga taaggttgta gcaggagata taattggtgg
     5281 cgttcaagaa acagcagtag tagaatgtaa aattatggtt ccttatggag ttaagggtac
     5341 tataaaagaa atatattctg gtgaatttac tgttgaagat acagtttgtg ttattacaga
     5401 tgaaaaaggt aatgatatac ctgtaactat gatgcaaaaa tggccagtta gaaaagaaag
     5461 accatataga gaaaagaaaa caccagattc acttcttgtt acaggtcaaa gagttataga
     5521 tacatttttc ccaatcacaa aaggtggggt agcagctata ccgggacctt ttggaagtgg
     5581 aaaaactgtt acacagcatc agcttgctaa atgggcagat gcagatatag ttgtatatat
     5641 aggctgtggc gagcgtggaa atgaaatgac agatgttctt cttgaatttc ctgaattaaa
     5701 agacccaaga acaggcgagt cacttatgca aagaactgtg cttatagcaa atacatcaga
     5761 tatgccagtt gctgcacgtg aagcttctat atacactggt attacaatag ctgaatattt
     5821 tagagatatg ggatatagtg ttgcacttat ggcagactct acatcaagat gggctgaggc
     5881 tcttagagag atgagtggtc gtttagagga gatgcctggt gaagaaggtt atcctgcata
     5941 cttaggttca cgtcttgctc aattctatga gagagcagga aaggtaaatt gtctaggtat
     6001 ggatgaaaga gaaggaacac ttacagcaat tggtgcagtt tctcctcctg gtggtgatac
     6061 atcagaacct gtcagccaag caacgcttcg tatagtaaaa gtattctggg gtcttgatgc
     6121 atctttagca tataaacgtc atttcccagc aattaactgg ttgactagtt attcactcta
     6181 tcaggaaaaa gttgatgtat gggcagataa aaatgtaaat gataatttct cagctcaaag
     6241 agctcaagct atgaaacttt tacaagttga atctgaactt caagaaatag ttcaattggt
     6301 tggggttgat tcactttcag atggagatag attgatacta gaggttactc gttctataag
     6361 agaagacttc cttcatcaaa actctttcca tgaagttgat acttatacat cacttcataa
     6421 acaatataga atgatgggac ttatattaaa attctataag tcagcacaag atgctatcaa
     6481 gcaaaatgtt actataaacg atattatcaa gctttcagtt cttgaaaaga ttggtcgtgc
     6541 taagtatgta gaagaaggcg atatagatgc agcttatgat tcaattgaag atgaaataga
     6601 caacgagctt gcagacctta ttaaaaagag gggggctgaa gaactatgat aaaggaatat
     6661 aagtcaatac aagaggtagc cggtcctctt atgttaataa acggcgtaga aggtgtaaaa
     6721 tttgatgaac ttggagaaat tgaactttca aatggagaaa ttcgccgttg caaagttcta
     6781 gaggtaaatg gagatactgc acttgttcag cttttcgaaa gctcaacagg tataaacctt
     6841 gctgaaagta aagttcgttt tttaggaaag agtcttgact ttggagtttc accagatata
     6901 ttaggccgtg tttttagtgg aatgggtaga cctattgatg gaggtccaga aattatacca
     6961 gataaaagac ttgatataaa tggagctcca ataaatccag cagcacgtga ttatccggcc
     7021 gaatttatac aaactggagt ctctgctata gatggactta atacattggt tagagggcaa
     7081 aaacttccta tattctcagg gtctggtttg ccacatgcca atcttgcagt tcagattgca
     7141 agacaggcaa aagtacgtgg aactgattct aagttcgccg ttgtatttgc tgcagttggt
     7201 ataacttttg aagatgctga attttttata agcgatttta aagcaactgg tgcaatcgac
     7261 cgttctgtag tgtttgtaaa ccttgcaaat gacccagctg tagagcgtgt atctacacct
     7321 agaatggcac ttacagcagc agaatacctt gcttttgagc aagatatgca tgtacttgtt
     7381 ataataacgg atattacaaa ctatgcagag gcattgcgtg aagtttcagc agctaaaaaa
     7441 gaagttccag gacgtcgtgg atatccagga tatctttata ctgaccttgc tactatgtat
     7501 gaaagagcag gaaaaatgaa agggcatgaa gggtctataa ctatgattcc aatacttaca
     7561 atgcctgagg atgataagac acatccaatt cctgacctta caggatacat tacagaaggg
     7621 caaattatac ttagccgtga actttataaa aaagatatac agcctcctat agatgtttta
     7681 ccatcacttt ctcgtctaaa agataaaggt attggtaaag gtaagactcg tgaagaccat
     7741 gcagatacaa tgaaccagct ttttgcagct tattcaagag gtaaggatgc aaaagaactt
     7801 atggctatac ttggtgaatc tgcactttct gagattgatt taatgtatgc taagtttgca
     7861 gatgaatttg agaaagaata tgtttcacaa ggatttagaa ctgatagaac tattgagcag
     7921 acattagaaa tcggatggaa gcttcttagt atgttaccaa aaagtgaact taaacgtatc
     7981 cgtgatgaat attttgaaaa atatatgcct aaggagtgat atctgtggct agattaaata
     8041 taaatccaac acgtatggaa atgactagac ttaagaaact ccttaaaact gctacaagag
     8101 gtcataagct tcttaaagat aaacttgatg aacttatgaa acaatttctt gagattgtaa
     8161 gagaaaataa gcgattgcgt gaagaagcag aaaatgctct tgatactgct tataaaaact
     8221 ttataatcgc aagagcagtt atgagtcagg agtatctggg ttcagcactt atgatgccaa
     8281 agcaatcagt ttcagtagat gtttctacga gaaatataat gagtgttgat gttcctgtgt
     8341 ttgactttaa aactgaaaac aatcaaagtg atatttatcc ttatggtctt gcttttacat
     8401 caggtgaatt agattctgct atggaagctt tctcagatgc tatgcaacca cttttaagac
     8461 ttgcagaaag tgaaaaaagt gcacagcttc ttgcacaaga gatagaaaag acacgtagac
     8521 gtgttaatgc tcttgaaaat gtaatgatac caaattacat tgagactatt aagtatatag
     8581 ctatgaagct tgaagaaaat gagagagcaa gtactactag acttatgaag gttaaggata
     8641 tggtgcttaa aaaagcactt gaagaaaaga aaaaaaatga tttggttgta taattaaata
     8701 cttataaaaa taagtctaac attctacttt atagaggtta gacttatttt ttatgtttaa
     8761 attaaaatgt agatactata aaaattaata tattgtttaa aaacagactg tatttaacta
     8821 aaaataatat atttaacaaa aatttctaaa aattaacgaa ctgttaattt acacttaact
     8881 ttatattaac aaattaggtg tattatataa acataagcaa aaacaataaa aaacaaataa
     8941 aaaaaggaaa aaacacaaag ggggaagata tgaaagaatt tatatttact tgtctgtgca
     9001 ttgtagtgat tacatttatg acaagttgca tatcaacaac accacaaact actcaaggaa
     9061 aaggaaataa taaacttaca atatatgcag cactagagga agagcagata gcttattata
     9121 tggaaggttt tagagaagag tatcccaata tagatgtaga gatagttatg gattctcatg
     9181 gtgtaatagc ttcaaaacta ttagcagaaa aagataatcc acaagcagat attatatggg
     9241 gattatcagc aatgaacatg atagagctaa aaaaagatgg tgcacttgaa gcatataagc
     9301 ctaaaaatta tgataaagtt gataaaaaat ttaaagatac tgaagatgat gtatcttggg
     9361 taggaatgag tgtcccagag acagcaatag tagttaatta taaggaactt aagaaactag
     9421 gtatagatat tccaaaaagt tatgaagatt taataaaacc agaatataaa ggacttataa
     9481 caatgccaaa tccagcatca tctggaacgg ggtatcttac agtatcaggg cttattcaaa
     9541 tgatgggtga aaaagatgct tacaattaca tggataaatt acataaaaat atagctacat
     9601 atacacactc aggttcaaaa cctgcgaagt tagctggctc tggagagtat ccaataggta
     9661 ttacacttgg atatagagct acacaacaat tatctagtgg agaacctata gaggttgtat
     9721 ttccaaagga aggttcaggt tgggacttag aagcaaatgc cttagttaaa aaagataata
     9781 taaaaaaaga agcaaagcta tttttagatt gggcaataag tgattcagct atggatagat
     9841 atgcacatga agtagcagtt acttccataa agacatataa tccagttcca aaaggttatt
     9901 ctaaacaacc atttgaacag cttataaaag aagttgattt aaactctgct gccaaaaaca
     9961 gaagtacagt acttaaaact tgggaatcaa aatacggttc aaaatctgaa agtaagtaag
    10021 gtttaaatta ataatttaga tgaaagaatt ttagaagaaa aagtaggata taaaatttaa
    10081 aataaaaaaa taactatata aggagagata agatatgagt tacttaaaaa taaataatgt
    10141 gtttaaaagt tatgaccaaa aaagggtttt aaataatatt agtttggata ttgaagaagg
    10201 ggaatttcta tgtttactcg gacctagtgg atgtggtaaa actacattac ttagaataat
    10261 agcaggtctt gaagatgtaa atagtggaac tataattctc caggataaag atattacaaa
    10321 tttagagcca tcaaagagag gttttggtat agtatttcaa tcatatgcat tatttccgaa
    10381 tatgactgct tataacaata tagcttttcc actaaaagag agaaaagttt caaaagaaaa
    10441 aatagataat aaagttaagg aagtacttga aacagttgga ttgactaatg aagctcataa
    10501 atatcctaaa gcattatcag gtgggcaaca acagaggatt gcaatagcaa gagcattagc
    10561 tcttgaacca aagtttttac ttttagatga accaatgtcc gcattagatg caaaggttag
    10621 acacaagctt agaatggaca ttaaaagatt acaaaaagaa cttaatataa cgacaataat
    10681 ggtaacacat gaccaagaag aagctataac tatggcagat aaaatagcta ttctaaatgg
    10741 aggagatata atgcaaatag ggactccaga agaaatatac caaaatcctc aaaacctatt
    10801 tacagctcaa tttataggag atacaaactg ttttgataat ggagattcta tattaactgt
    10861 aagaccagaa tatgttcaaa tagagaaatc gacaaaagaa aactaccaag gtataatatc
    10921 aaatatagag tttagaggga atttattgag agttgaaata aaagataaat taaatgaaaa
    10981 tttcataata agtgatgttt ctataaaaga gtgggttaat ttaaatttag tagaaggtga
    11041 ccttgtaaaa ataagtatag acgaaaaata ctatttaaag tatccaaaag ttaaaggagc
    11101 ataagtgtat gaataataat gctataagtt ctaaaagtat aagtcttaaa tcaaaaatga
    11161 atttagatat aaaaaaatgt atgcttacag tattagttat gtctttagta atatttttat
    11221 tagcacctat aatatatcta tttgtacagg cttttttaga taaagatgga gtctttgtag
    11281 gattacaaaa ctttcaagaa tacttggctt ctgaatctct attaaattct ctaaaaaata
    11341 cagtatttgt atcagttata tcaactgtaa tatcactaac acttgcatta gtatatgcat
    11401 atgcattaac tagaacaaag attaaattta aaggtttttt taaatcatta gctatgtttc
    11461 ctatgtttgc tccaacaatg atgcatgcaa taggtctagt atatttattt ggaaatcaag
    11521 gtttggtaac taatttactt ggagtgcatg ttccaatata tggagcagtg ggaataatat
    11581 tggcacaaat tatatatgta tttcctcaat cctttcaaat actttatata tctctaagtt
    11641 caaccgatta tagactatat gaagctagta atactatggg gataagcaag ataagagaat
    11701 ttataagcat aacaatccca agtataaaat actctttaat aagttctgca ttcgtatcat
    11761 ttacattatg ttttacagat tttggagctc caaaggtgat aggaggagag tttaatgtat
    11821 tatcaataga tgtatataag caaattatag ggcaacagaa tatgccaatg ggagcaactg
    11881 tatcaatatt acttttaata cctgcaataa tatcgtttat agtaactaaa aaaattgaaa
    11941 aaaatcaaaa ctcatatata acttcaaagt caattccata taaggttaaa actaataagt
    12001 taagagatag tattttagga ggggttgtag gaattattgc atttggtata atagctgtaa
    12061 tgttaactgt aatcttatcc tcattaatat cagtttggcc atatgattta agacttacac
    12121 tggaacactt ttctatgaaa aacagtattg atggaattaa accatttata aattcaataa
    12181 aaatgtcact attgacagct attctaggga cgagctttgt tttcttattt gcatatttaa
    12241 atgaaaaaac agaaaaagct ccatatttaa aatcatttgg gtatttttta agtacattgc
    12301 ctatggcatt gccaggatta gttttaggta tagcttatgt attatttttt aataaattag
    12361 agtttaattt tatggataca atatacatta aaaatttgtt taatggatta tatggaacat
    12421 tacttataat ggttatatgt aatatagttc atttcttttc agtagcattt ataacagcta
    12481 ctactgctat taagaaagta gataaagaat tggacatggt agctaaatca atgggaataa
    12541 acagcgcatc aattcttaag aacgtaacta taccactttg tttgccagca gttttagaga
    12601 actttatgta cttttttcta aactctatgg tgacaatatc agcactagtt tttatatata
    12661 ctgcaagttc aaaagtagct gcaatatcaa ttgtacaact agatgataag gggagtactg
    12721 cacaggcagc agcactagct gtgttaatat tagtcacaaa tatattagtg aaagtaatat
    12781 atgaatttat aaagaatcgt ttagaaaaaa gaacaaactt taattaaata atttatctta
    12841 taaaaacaca aatttaaaaa gtctattctt atgatttatt atttaaagaa tagacttttt
    12901 aaatttatta ttgatattat ttattaattt tctttttgtt atcatacatt tgagaatctg
    12961 cacgacgata tgtatcaaat aaagaaacat ctactttttc atcaaaaata gcatatccaa
    13021 tagacattga taattttaat tcattagttg agttttcttc aactattttt aaattaatat
    13081 tatttataat actatttatt tctttgtgag ataaattttt tagaataatt atgtattcat
    13141 cacctccaat acgataaact atatattttt tatttattac atcttgtata attttacttg
    13201 cttgcttaat atataagtct cctgtctgat ggccaaattt atcattaatc ttttttaaac
    13261 cgtctaaatc tattgatatg atagatatta gattctttag atttgaattg tttaagttat
    13321 tcatatctat ttcaaaagca tttctatttt ttaggcctgt taaataatct gtattagcaa
    13381 aatcagtata tgtaggatta gatattcttt taaatagaat cactgcaatc aatgctgata
    13441 ttagacaagt taataatgct attattggtg taactatact tataaattta aatgtattgt
    13501 attgatgttt tgcttcaaat tcaattccta ttactccaac aaccttacca acatcattgt
    13561 gaataggaaa atacgatata aatatgtatc cccaagatgt tgatttaatc tttttaggta
    13621 acaatatatg attatctaat gctttgttaa gatctcccca aatctctttt tctatcaaat
    13681 caccagcatt tctaaaatca cttctagatg aatttaaacc atcaacaaca tatactagtg
    13741 atccatcttt tgttttttta gcagtatata gatacatgac tccagtagta ttttttacag
    13801 attctaatag gattttagaa cttttataag acttttttga ttggtcttct ttagaattaa
    13861 tttcagtaaa ggtattttta tcaatatttc tctctacata ttcatagata tgttttgacc
    13921 tctcacttaa agagtatatc atgctcttat aagttaatgt ataattaaaa aaatatacac
    13981 atgaggaaga tattattaca ataattgcag taagtattga aacctgcata tcaactctat
    14041 taaatttttt atttattttc atagcttaaa tgtcttgtga aaagaacctc ccacaatctc
    14101 cccataatta atatatatct ataaatttca ccataatata ttcatatatt atcaaatttg
    14161 tgtggaaatt acaattagta aacatgctta aagtattcaa atatagtttt tttattattg
    14221 cagtatacta ttttatgtat aaataaaatt atagataaat cacttatatt tgttatttct
    14281 taatggaaga tacattataa aatattctaa ttgattacag tatgtatact taattgaagt
    14341 taagttaagg gttataatat gtattgatga aatgactata tttatattca taagtattaa
    14401 ttttatcaga gataggtaaa atactttatc ttaaacataa gtatggttga gatgcaatct
    14461 tgagatatga taatataaat tgaaggatag tgaactcaat caaataaaat gattaatcta
    14521 gctatataag caaataaatt taatataaaa gaggtggaaa atgaaagaca gaattaattt
    14581 aacagaagga agaattacag aaaaattact taagctatca cttccaataa tggggacttc
    14641 atttatacag atggggtata acatgataga tatgatatgg gttggtaaag ccggaagtaa
    14701 agcagtagct gcagttggaa cagctggttt ttttccatgg cttgcaatgg catttattat
    14761 gatttcaaaa gtaggtggag agataaaagt agcacaaagt attggtgcaa ataatataag
    14821 cactactaaa tcatatatga agtcagcaat agaaataaat attatactag caataatata
    14881 tacaatttct ttaatagtac ttaataaaca attgataggt ttttttagat taggagactt
    14941 agaagttata accatgtcta gacagtattt gataatagta gctctaggta tggtatttta
    15001 ttttattaat ccagtattta ctgcaatttt caacggtctt ggaaatagta aaactccttt
    15061 tcgtattaat acagtaggat taataactaa tattatactt gatcctatat taatttttgg
    15121 atggggattt gctccaaaac ttggtgttgc aggagctgct attgcaactg tatttgctca
    15181 gatagtagtt actacatgtt ttatatatat aatagttaaa agtaaagaag aatattttaa
    15241 attaagacta tttaaagaaa tagagactaa atattataaa gttctgtata aattgggatt
    15301 acctatagca atacaaagtg gtatgtttac agtattctca atgctattag gagtaatagt
    15361 agcatcatgg gggcctgtgg ctgttgcagt acaaaaagta ggctcacaaa tagaagcaat
    15421 ttcctggatg acggctgaag ggctatcagt tgcattaggc agttttgttg gacaaaatta
    15481 tggtgccaag aagtattcta gaataaataa aggttgcaaa atagctataa ctgtatcttc
    15541 tgtacttggg ataataacta ctttggtatt agtatttgca ggagagagta tattttctat
    15601 ttttatagat gaaagtgagg caatagaaaa aggagcaatt tatcttaaaa tattaggcta
    15661 ttctcaattt tttatgtgtt tagaaataat aacaacagga gctttcaaag gattaggcag
    15721 aacctatata ccttctatta taattactat actgactggt gcaagagtac cattggctta
    15781 ctttatatct agaccagaaa tgttgggtct aaatggtgta tggtggagta taaccttatc
    15841 aagtatatta aaaggtatat taatgataag tctatttata tctttattaa aactcggaaa
    15901 gttgtacaag atgtctgaat aaaatttaaa ttatcttaaa tgtatagaca ttttctttat
    15961 ttgtatataa aattatctta ttatataaaa gatttaatta ataaggagtg aattaaatga
    16021 aagagccaat ttataaagtt atagaaaatc atgttagaga gttaataaat tcagacagtt
    16081 tgaaagaagg ggatttaatc ccttctgaaa aacagctgag tgaagaattt aatgtaacta
    16141 gaatgactgt aaggtctgca cttaataatc tagttaaaga aggctatata actagacaaa
    16201 gaggtgtagg tagtatagtt attgcaaata atatatatga taatatatcg tctgtaagtg
    16261 gatttactaa agagatggaa agtaaaggct ataaagtatc taatatattg gtatctcttg
    16321 aaatagttca ggcagatgaa aatttatcaa gtaagttgaa tataagttta gaagaaaatg
    16381 tttgggaaat aaaaagagta agattagcca atgatgcaag agtttcatat atgattactt
    16441 atatgccagt taagttattt ccaaatttaa ataagactca ttgtgaaaat tctctttata
    16501 attttgttga agaagtgtgt ggatacaaga tagccatgtc agagagagaa gtacaagctg
    16561 taatatcgaa tgaagaatgt atggataatc taaaattaga agaaccagag ccacttttat
    16621 atataagcca aatttgtaag ttacaaaata gtgagatttt tgagtactca cacacatatc
    16681 actatggata tacattaact ttgaatgctg ttgtcgaata aaatttaaat aaaatgaaaa
    16741 agttttcttg aaatgtatat acatttggta tataataata tcaagaatta aatgtatata
    16801 catttaaaaa taagatatat acattaagaa aaaattaata gaaaagcata ttttaaatgt
    16861 atagtttaaa aaatttatga cttaaaaaat atttaatgaa tctaatttgg aggggaaaaa
    16921 atgataaaaa taatgttagc ttgttcagca ggaatgtcaa ctagtttatt agttacaaaa
    16981 atggaggatg cagcaaagga aaacggtata gaagcaaaaa tatgggctat atcagaagtg
    17041 aacttaaaaa atgaaataga aaattgtgat gtcttacttt tagggcctca agttagatat
    17101 gtattaggaa aagcaactga aatggcaaaa ccatataata ttccagtaga agttataaat
    17161 atgatggatt atggaagatg taatggaaaa gctgtgttag atagagctgt agagttaaat
    17221 agttcaaaat aaagttgtac tggaggatgt aaaatggaaa aattcatgtc atttatggat
    17281 aaatatatag tgcctgtagc tgctaaaata ggagctcaaa ggcacttagt agcagtaaga
    17341 gatgcattta tagtaatgat acctataact atggtaggtg ccttgggaac attgataaat
    17401 aacttaccat tggaagcata caaaaatttg atggcaagta tatttggaga aaattggacc
    17461 acatttggag gcgacctttg gtggggagca ataggtacaa tggcagtatt cttagtaata
    17521 ggtgttgctt attttctagc aaaatcttat gagagtgatg ggttacaatc tgggcttata
    17581 gctttatcta tcttttttat aatggctcca caaattggta aaatagttcc tgagggagga
    17641 actacagtag ttgaaggatg gggaatgata caacaaacct acttaggtac agcagcttta
    17701 ttctcttcta tattaatagg attattatca actgagatat ttgtaagatt atctaaggta
    17761 aagaaattaa ctataaaaat gccagatgga gttccaccag cagtttcaag atcatttgca
    17821 aagttaatac caggaatgtt aactataatg atatttactg taataggaat atttataaaa
    17881 atgctttcta atggaagttt cttaacagat attttaaata cttatttagg agcaccgcta
    17941 tcaaatgtag ctgatagtct aggttctaca atgcttattg catttataat tcatatactt
    18001 tggactgttg gacttcatgg tgcaaatata gcacttcctt ttacagaaac tatacttatg
    18061 aaattaggtg gagaaaatgc tgccttggct caagcaggag caacggaagg ataccatgta
    18121 cttgcaggtg cattctttga tgcatttgta tatttaggtg gttctggaat ggtattggga
    18181 ttaatagtag ctttattaat agctgggaga agacgtaaag aaatgatagt attaggtggg
    18241 ccacctgcaa tatttaatat aggagaacca ttgatattcg gacttccaat agttttaaat
    18301 cctatattta tgattccatt tgtacttgca ccagtgatat gtagtgctgt atcatactta
    18361 gcaatagatt ttggattagt agcaccagta attttaccta aaataccttg ggtaacacct
    18421 cctatattag gtggagcgat ggctacaggt gactggactg gtggagcttt agcactattt
    18481 aatttaatat tatcaatatt aatatatatt ccatttgtca tagcgtctga aaaaatggaa
    18541 gctaataaat tgaaaattaa caactaggta gttttatttc ggtaaataaa tgtatagggt
    18601 tttgtagagt atattaaatt ataggtaagg atggtataat gagatgaaaa agggactaaa
    18661 aatagttaca attggaggag gttcaagtta tactcctgag cttgtagagg gttttataaa
    18721 tagatatgaa gagttacctg taaaggaatt atggcttgta gatatagaag ctggaaaaga
    18781 aaaattagag atagttggaa atctagctaa gaggatggta aaaaaagctg gagtagatat
    18841 gaaaataaat cttactttag atagaagaga agctctaaga gatgcagact ttgtgacaac
    18901 acaacttaga gtaggtcttt tagatgcgag aataaaagat gaaactattc ctttatctca
    18961 tggagttatg gggcaggaaa ctaatggtgc tggaggttta tttaaagccc ttcgcacaat
    19021 accagttata tttgatataa taaaagatgt ggaagaatta tgtccaaatg catggatggt
    19081 gaattttaca aatccaacag gaataataac agaggctgta ttcaaatata ctaattttag
    19141 aagatatata ggtctatgca atgtgccaat tcatttaaaa aatgatgttg ccaaactatt
    19201 taatgtagaa agtgacagaa taagtatgga ttttgcaggt cttaatcata tggtttatgg
    19261 tctaaatgta tctttagatg gagaagatgt tacaaaagaa gcaattgata agtttgtcaa
    19321 agcagatata agtatgcaaa atataaaggc aattgatttt aatgctgaat ttgtaaaatc
    19381 tttgggtgca ataccttgtc catatcatag atactactat aaaactaaag aaatgcttga
    19441 ggatgagttg agagagttta aagagggaaa agctagaggt caagtagtta aagaattgga
    19501 agaacaatta tttgaattat ataaggatga aaaattagat gtaaaaccac ctcaacttga
    19561 aaaaagagga ggggcttact atagtgatgc tgcttgtaat ttaataagtt ctatatacaa
    19621 tgacaaaaaa gatatccaag tagttaatac attaaacaat ggttctataa gagattttaa
    19681 agatgaacaa gcggtagaag tgagtagtgt aataaccaaa aatggtccaa aaccaataag
    19741 tataggatat ttaccagatt ctgtgcatgg attagtaagc caaatcaaat catttgaagt
    19801 attggtagct aaagcagctg tatatggcga ttatgaatct gctcttttgg cactttgtat
    19861 aaacccattg attccatctg atgacttagc aaaaactata ttagacgaaa tgttggaagc
    19921 tcataaagat tatcttccta gatttaatag atagttgtct atagaaagta ggttatggta
    19981 tgactaaaat tataataaat gcagatgatt ttggatattg tgaagctgtg aactatggaa
    20041 taatttcagc tcataataat ggtatagtta ggtctacttc tatgatggca aatatgcctg
    20101 gggtagaaca tggagtaggc ttacttaaag aaaataaaac tttaaattgt ggagttcata
    20161 tgactttaag ttgtggtaga ccattattat ctaacttaaa aactattgtt gataaagacg
    20221 gtttttttat taggaggatt acagacgaaa ttattgaaaa aatggattgt gatgagattt
    20281 atagagaact ttgtgctcaa atagataggg taaaagggct tggaatagat atatctcatt
    20341 tagatagtca ccatcatatc catactttag taagtttaaa gccagtagta gagaagattg
    20401 taaccaagta taatcttcca ataagaggtg gatttgaata taatttagag tattctaaag
    20461 tagttccttt aatagatagt ttttataagg aaaatgtaag tgaagaatat tttataaaaa
    20521 atatagacga aataatgaag tatgatgtag tggatattat gtctcaccca gcttttttag
    20581 atgattatat tttaaattct acatcttatg ctattgatag aacgaaagag cacaagatac
    20641 ttacatctaa aaaagtcaaa gaatttttag aaaagaatgg attagtcata tcaagctata
    20701 gagatattta ggaggtcgaa taaatggatg agaagatgat agagatatct tttggaataa
    20761 ttggatttgc aggtgatgcg aaagggctag catttgaagc aataaaagaa gcaaagagcg
    20821 ggaatataga taaagctaga gaatgtattg aaaagtcgaa agaatcaatt cataaagctc
    20881 ataaattcca aacagaattg atacaaaatg aagctaatgg taataaaact gaaataagtg
    20941 ttatacttat tcatgcacaa gaccatttaa tgacagctat gaattttcaa caattagcag
    21001 aagaatttat agatgtatat gaaagattag aaagta
//
LOCUS       DAEPOZ010000042        20911 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.042, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000042 DAEPOZ010000000
VERSION     DAEPOZ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20911
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.042"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            358..669
                     /gene="rpsJ"
                     /locus_tag="KR440_001233"
     CDS             358..669
                     /gene="rpsJ"
                     /locus_tag="KR440_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBH0698291.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            761..1390
                     /gene="rplC"
                     /locus_tag="KR440_001234"
     CDS             761..1390
                     /gene="rplC"
                     /locus_tag="KR440_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBH0698292.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1420..2043
                     /gene="rplD"
                     /locus_tag="KR440_001235"
     CDS             1420..2043
                     /gene="rplD"
                     /locus_tag="KR440_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBH0698293.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2043..2333
                     /gene="rplW"
                     /locus_tag="KR440_001236"
     CDS             2043..2333
                     /gene="rplW"
                     /locus_tag="KR440_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBH0698294.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTASFKKAVVKLTADSKEIEFFQGM"
     gene            2363..3193
                     /gene="rplB"
                     /locus_tag="KR440_001237"
     CDS             2363..3193
                     /gene="rplB"
                     /locus_tag="KR440_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBH0698295.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3228..3509
                     /gene="rpsS"
                     /locus_tag="KR440_001238"
     CDS             3228..3509
                     /gene="rpsS"
                     /locus_tag="KR440_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBH0698296.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3541..3876
                     /gene="rplV"
                     /locus_tag="KR440_001239"
     CDS             3541..3876
                     /gene="rplV"
                     /locus_tag="KR440_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBH0698297.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            3899..4714
                     /gene="rpsC"
                     /locus_tag="KR440_001240"
     CDS             3899..4714
                     /gene="rpsC"
                     /locus_tag="KR440_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBH0698298.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            4750..5181
                     /gene="rplP"
                     /locus_tag="KR440_001241"
     CDS             4750..5181
                     /gene="rplP"
                     /locus_tag="KR440_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBH0698299.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5183..5386
                     /gene="rpmC"
                     /locus_tag="KR440_001242"
     CDS             5183..5386
                     /gene="rpmC"
                     /locus_tag="KR440_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBH0698300.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5410..5664
                     /gene="rpsQ"
                     /locus_tag="KR440_001243"
     CDS             5410..5664
                     /gene="rpsQ"
                     /locus_tag="KR440_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBH0698301.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5690..6058
                     /gene="rplN"
                     /locus_tag="KR440_001244"
     CDS             5690..6058
                     /gene="rplN"
                     /locus_tag="KR440_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBH0698302.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6082..6387
                     /gene="rplX"
                     /locus_tag="KR440_001245"
     CDS             6082..6387
                     /gene="rplX"
                     /locus_tag="KR440_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBH0698303.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6417..6959
                     /gene="rplE"
                     /locus_tag="KR440_001246"
     CDS             6417..6959
                     /gene="rplE"
                     /locus_tag="KR440_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBH0698304.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            6977..7162
                     /locus_tag="KR440_001247"
     CDS             6977..7162
                     /locus_tag="KR440_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBH0698305.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7195..7593
                     /gene="rpsH"
                     /locus_tag="KR440_001248"
     CDS             7195..7593
                     /gene="rpsH"
                     /locus_tag="KR440_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBH0698306.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7624..8166
                     /gene="rplF"
                     /locus_tag="KR440_001249"
     CDS             7624..8166
                     /gene="rplF"
                     /locus_tag="KR440_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBH0698307.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8202..8570
                     /gene="rplR"
                     /locus_tag="KR440_001250"
     CDS             8202..8570
                     /gene="rplR"
                     /locus_tag="KR440_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBH0698308.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8591..9100
                     /gene="rpsE"
                     /locus_tag="KR440_001251"
     CDS             8591..9100
                     /gene="rpsE"
                     /locus_tag="KR440_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBH0698309.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9115..9300
                     /gene="rpmD"
                     /locus_tag="KR440_001252"
     CDS             9115..9300
                     /gene="rpmD"
                     /locus_tag="KR440_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBH0698310.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9333..9776
                     /gene="rplO"
                     /locus_tag="KR440_001253"
     CDS             9333..9776
                     /gene="rplO"
                     /locus_tag="KR440_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBH0698311.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            9820..11088
                     /gene="secY"
                     /locus_tag="KR440_001254"
     CDS             9820..11088
                     /gene="secY"
                     /locus_tag="KR440_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBH0698312.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11105..11755
                     /locus_tag="KR440_001255"
     CDS             11105..11755
                     /locus_tag="KR440_001255"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBH0698313.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            11756..12502
                     /gene="map"
                     /locus_tag="KR440_001256"
     CDS             11756..12502
                     /gene="map"
                     /locus_tag="KR440_001256"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBH0698314.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12524..12793
                     /locus_tag="KR440_001257"
     CDS             12524..12793
                     /locus_tag="KR440_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HBH0698315.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYDFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            12831..13049
                     /gene="infA"
                     /locus_tag="KR440_001258"
     CDS             12831..13049
                     /gene="infA"
                     /locus_tag="KR440_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBH0698316.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13085..13198
                     /gene="rpmJ"
                     /locus_tag="KR440_001259"
     CDS             13085..13198
                     /gene="rpmJ"
                     /locus_tag="KR440_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBH0698317.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13351..13722
                     /gene="rpsM"
                     /locus_tag="KR440_001260"
     CDS             13351..13722
                     /gene="rpsM"
                     /locus_tag="KR440_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBH0698318.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            13758..14156
                     /gene="rpsK"
                     /locus_tag="KR440_001261"
     CDS             13758..14156
                     /gene="rpsK"
                     /locus_tag="KR440_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBH0698319.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14188..14811
                     /gene="rpsD"
                     /locus_tag="KR440_001262"
     CDS             14188..14811
                     /gene="rpsD"
                     /locus_tag="KR440_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBH0698320.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            14889..15836
                     /locus_tag="KR440_001263"
     CDS             14889..15836
                     /locus_tag="KR440_001263"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBH0698321.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            15857..16198
                     /gene="rplQ"
                     /locus_tag="KR440_001264"
     CDS             15857..16198
                     /gene="rplQ"
                     /locus_tag="KR440_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBH0698322.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16318..17151
                     /locus_tag="KR440_001265"
     CDS             16318..17151
                     /locus_tag="KR440_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBH0698323.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17139..18005
                     /locus_tag="KR440_001266"
     CDS             17139..18005
                     /locus_tag="KR440_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBH0698324.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDISFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            17999..18802
                     /locus_tag="KR440_001267"
     CDS             17999..18802
                     /locus_tag="KR440_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBH0698325.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            18834..19565
                     /gene="truA"
                     /locus_tag="KR440_001268"
     CDS             18834..19565
                     /gene="truA"
                     /locus_tag="KR440_001268"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBH0698326.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19685..20116
                     /gene="rplM"
                     /locus_tag="KR440_001269"
     CDS             19685..20116
                     /gene="rplM"
                     /locus_tag="KR440_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBH0698327.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20145..20537
                     /gene="rpsI"
                     /locus_tag="KR440_001270"
     CDS             20145..20537
                     /gene="rpsI"
                     /locus_tag="KR440_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBH0698328.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
BASE COUNT         8354 a         2452 c         4347 g         5758 t
ORIGIN      
        1 atggatttaa tataaaaaaa agaggggggt aactcctctc ttttttgttt tttgttgaca
       61 agtagcgcta aagcatatat actaaactag tgccttgaga cagattaaca atcaagttta
      121 aggttatgat gttgtaatag aaaataaaat tgaaaaaaac agaaaaaaaa cttgaaaaaa
      181 tgtaacagat gatataaaat ataatagtgt gcttgagaga ttaagaaaat aataattaaa
      241 tcctagtgaa atacgaactg atatcgattt aaaagttaca acacacccgg agcagtgtat
      301 cggtattggt tgtacgcaac ttgaaaatta aacacgtgat aaaggaggga aaacaaaatg
      361 gctaagaatg aaaaaataag aataagatta aaatcatatg atcacaaatt attagatttt
      421 tcagcaggta aaatagttga aactgctaaa aaggctggat cacaagtttc aggacctgtg
      481 cctctaccaa cagaaaaaca agttgtaact atattaagag ctgtacataa gtacaaagac
      541 tctagagaac aatttgaaat aagaactcat aaaagactaa tcgacatagc taatccaaca
      601 cctaagacag ttgactcatt aatgagatta gacttaccag caggtgtaga tatagaaata
      661 aaattataag aacccctaag atattatcct atagatgatt atcttagaat gattactcta
      721 aaagatgtaa tccgctgtaa gaattatagg aggtgctaat atgaaaggaa tattaggcaa
      781 aaaagttggt atgactcaaa tattcactga taaaggtgta gttatacctg taacagctgt
      841 tgaagcaggg cctatggtcg ttactcaaat aaaaacagtt gataaagatg gatacaatgc
      901 tatacaaata ggttttgaag atgctaaaga aaaagcttta aacaaaccta aaaaaggaca
      961 tttagctgct gctaatgttt taaagaaaca tttaaaagaa tttagagtag attctgtaga
     1021 aggatacaca gtaggacaag aaataaaagc tgatgtattt gaagcaggtg caaaaataga
     1081 tgttactgga ataagtaaag gtaagggatt ccaaggtcca ataaaaagac atggacaaag
     1141 tagaggtcct gaaactcatg gttctagata ccacagaaga ccaggttcaa tgggagcttg
     1201 ttcttaccca ggtagagtat ttaaaaacaa aaaattagct ggacatatgg gaagcgtaaa
     1261 ggtaacagtt caaaacttag aggttgttaa agtagatgct gacaagaacc ttatattagt
     1321 taaaggagct ataccaggag ctaaaggttc agtagtaact ataaaagaag ctataaaggt
     1381 ttctaaataa tgactaacct agagaaagga ggaataacaa tgccaaaatt aaatgtacta
     1441 aatgttagtg gacaaaacgt tggagaaata gaattatcag attctatatt tggcgtagaa
     1501 gttaatggac atgttttata cgaagttgta aaaaatcaat tagcaaataa gagacaagga
     1561 actcaatctg ctaaaactag agcagaagtt agaggtggcg gaagaaaacc ttggaaacaa
     1621 aaaggaacag gtagagcaag acaaggttct acaagatctg tacaatgggt aggtggagga
     1681 gttgcttttg cacctaaacc aagaagctac aaatatacat tacctaagaa ggtaagaaga
     1741 ttagctatga agagtgcttt atcttcaaaa gttcaaaata gtgaagttat agtattagat
     1801 gcattaaaca tggatgctcc taagactaaa gaatttgctc aaatattaaa caatataaat
     1861 gctgctaaaa aagctttagt agttatagct gacaaaaacg ataacgtaat aaaatcagct
     1921 agaaatatag aaggtgttca aactgcatta gttaatacta tgaatgtata tgatatatta
     1981 aaatacgatt catttataat aactacagat gctgttaaaa aagtggagga ggtgtacgca
     2041 taatgactaa tccacatgat gtaataataa gaccagttgt aactgagcac agtatggctg
     2101 aaatgggtga aaaaaaatac acttttgttg tggctaaaga tgcgaacaaa actgaaataa
     2161 aaaaagcagt agaaaaagta ttcggagtta gtgttgataa agtaaatact ttaaactacg
     2221 atggaaaagt taaaagaatg ggtagaactc aaggaagaac agcaagcttt aagaaagcag
     2281 tagttaaatt aactgctgat agtaaagaaa tagaattctt ccaaggaatg tagtattata
     2341 acaaacgaag gagggaaaac agatggctat aaaaaagttt agaccaactt ctcctgcctt
     2401 aagacaaatg acagttttag tttctgatga aataacatgt aatcaaccag aaaaatctct
     2461 tttagttaac ttaaagaaaa atgctggtag aaacgtacat ggtagaataa ctgttcgtca
     2521 tagaggtgga ggacaaaaaa gaaaatatag aataatagat tttaaaagag ataaagatgg
     2581 tatacctgct aaggttgcta ctatagaata tgatccaaac agaactgcaa atatcgcatt
     2641 attaaactat gctgatggag aaaaaagata tatattagct ccagttggaa ttaacgttgg
     2701 agatactata ttatcaggat taggtgctga tataaaacca ggaaactgtt tagcattaaa
     2761 agatatgcca gttggtacta tcattcataa tatagaatta aagccaggaa aaggagctca
     2821 attagttaga tctgctggag tttctgcaca attaatggct aaagaaggaa aaaatgcttt
     2881 attaagatta ccatcaggtg agatgagatt agtaagcata aactgtaaag ctactatagg
     2941 tcaagttgga aatatagagc atggtaacgt agttatcggt aaagcaggta gaaaaagaca
     3001 tatgggaata agacctactg taagaggttc tgtaatgaac cctaatgacc atccacacgg
     3061 tggaggggaa ggtagatctc caataggtag accatctcct gttactccat ggggtaaacc
     3121 agctcttgga tacaaaacta gaaagaaaaa taaagcttca aataaactaa tagtatcaag
     3181 aagaactaag tagtaaattt atttttgctc ggaaaggagg aatataaatg tcaagatcaa
     3241 ctaaaaaagg gccttttgtc catgcaagac ttttaaagaa gatagaagct atgaatgcta
     3301 gtggaaataa agaagttata aaaacttggt caagatcttc aactgtattt ccacaaatgg
     3361 tagaaaacac tatagctgtt catgatggaa gaaaacatgt tccagtatat ataacagaag
     3421 atatggttgg acataaatta ggtgagttcg ttcctactag aactttcaaa ggacataagg
     3481 atgacgaaaa atctaataag agaaaataat taaattatga ctaagaaagg aggaataaca
     3541 atggaagcaa aagctactgc taaatacgta cgtgtatcac caagaaaagc aggccaaata
     3601 tgtgaccttg ttagaggaaa gaatgttgat gaagcattag caatattaaa gtttactcca
     3661 agaggagcag cttcaataat agctaaggtt gtaaaatcag ctaaagctaa cgcagaaaac
     3721 aatcacgaaa tggatactga aaaattatat atagcatcaa tagttgctaa tcaaggacca
     3781 acaatgaaga gattcatgcc tagagctatg ggtcgtgcaa ctacaataag aaaaagaact
     3841 tctcatatag aggttgttgt taaagaaaaa aaataattaa tagataggag ggaaagtaat
     3901 gggtcaaaag gttaatccac acggcttaag ggtcggtgtt ataaaagatt gggactcaag
     3961 atggttcgct actgataaaa aagagttcgg aaacttatta ttagaagacc ataatatacg
     4021 taaattctta aagaaaagat tatactcagc tggagttgct aagatagaaa tagaaagatc
     4081 agctaacaaa ataaaaatgg acttacacgt tgctaagcct ggtgtagtta taggaagagc
     4141 tggtgcagga atcgaagcat taaaagctga attagaaaaa atgacaaaga aaactataat
     4201 agttaatata gtagaagtaa gaagtacaga taaaaatgct caattagtag ctgaaaatat
     4261 agctttagct atagagagaa gggttgcttt cagaagagca atgaaacaag ctatacaaag
     4321 agcaatgaaa tctggtgcta aagggataaa agtttctgct tcaggaagat taggtggagc
     4381 tgaaatggct agaactgaag gttatagtga aggaaatgtg ccactacaaa ctttaagagc
     4441 agatatagat tatggtttcg ctgaagctga tacaacttat ggaaaaatcg gtataaaagt
     4501 ttggatatgt aatggtgaag ttttaccaac tagagacggt gtaaatccaa gagaagaaag
     4561 cagaaagagc gatagaagag acaacaagag agataataga agaaacgata gaagaggaaa
     4621 tgacagaaga ggaaatgata acagaggaaa ctacagagga caaagacctc aaggtggatc
     4681 aagacctcaa agaactgaaa acaaaggaaa ttaataatag ttaggttcaa ggaaggagga
     4741 aaaatactca tgttaatgcc aaaaagagta aagcgtcgta gagttcatag aggaagcatg
     4801 gctgggcaag ctcataaggg taataaagtt acttatggtg agtttggatt agtagcatta
     4861 gaagcttctt ggataacttc taatcaaata gaagctgcca gaatcgcgat gactagatat
     4921 ataaaaagag ggggaaaggt ttggataaaa atattccctc ataaaccagt aacaagaaaa
     4981 ccagcagaga ctcgtatggg tgcagggaaa ggttctccag aatattgggt agctgtagtt
     5041 aagccaggaa gagttatgtt tgaattagca ggtgtttctg aagataaagc aagagaagct
     5101 atgagacttg ctgcacataa attaccaatc aaatgtaaat ttgttaaaaa agaagattta
     5161 gaagtaaagg gtggtgaata ggatgaaagc taaagaatta agagatttaa caagcgaaga
     5221 gctaatgaac aaattaaacg acttcaaaag tgaattattt agcttaagat tccaattagc
     5281 tactggtcaa ttagaaaata cagctagaat aaagtttgtt aagaaggata tagcaaaagt
     5341 taaaactgtt cttgctgaaa gaaagttata cgaaactaga gcttaatttg gaaaggaggc
     5401 tttttaaaca tggaaagagg aagaagaaaa gttagaatag gccgtgttgt tagtgataag
     5461 atggataaaa caatagtagt agctgttgaa gaattcgtac gtcatccact atataataaa
     5521 cgtgttaaga gaactaagaa attcaaggct catgatgaaa agaatatatg taacatcgga
     5581 gatagagtta aaataatgga aactagacca ttatctaaag ataagagatt cagactagtt
     5641 gaagttgttg agaaagttaa gtagtttttg aagaaggagg gattacgata tgatacaaca
     5701 agaatcacgt ctaagagttg ctgataactc aggagctaaa gaacttttat gtatacgtgt
     5761 tctaggcgga agtaaaagaa gatatggtaa cataggcgac gttatagttg ctactgttaa
     5821 aagtgcaaca cctggtggag ttgtaaaaaa aggtaaggtt gttaaagctg ttatagtaag
     5881 aagcaaacaa ggcgtaagac gtaatgacgg aagctatata tcttttgatg aaaatgctgc
     5941 tgttataata aaggacgata aaactccagt aggaactcgt atattcgggc ctgttgctag
     6001 agagttaaga gacaatgaat tcatgaaaat agtttctctt gctccagaag tactataatt
     6061 aaggaggtgc aataggacat gatgcgtgtt aaaaaaggtg acactgttgt agttatagca
     6121 ggtaaagata aaggtaaaaa aggttcagtt ttaaaggtat atcctaaaac tagcaaagtg
     6181 ttagttgaag gtgtaaatgt aataactaaa catcaaaaac caagtgctat gaaccaacaa
     6241 ggtggaataa taaataaaga agccccaata cacatatcta atgtaatgcc atttgatcct
     6301 gaaacaggaa aaggcgttag agtaagatat gaagtaaaag atgggaacaa agtaagagta
     6361 tcagcaaaga gcggaaaaga attataataa atattgcttg aaaggaggga ccttaaatgg
     6421 cttctagatt acaagaaaaa tacatgaaag aagttgctcc tgctttaatg gagaaatttg
     6481 gatacaaaaa cgtaatggag atacctaagt taaataaaat agttattaac atgggtatag
     6541 gtgacgctag agaaaatcca aaaggattag aaaaaggcgt tgaagaatta gaaatgatat
     6601 caggacaaaa gcctgttata actaaagcta gaaaatctgt tgctaacttc aaattaagag
     6661 aaggaatgcc aataggaaca aaagttacat taagagctga caaaatgttc tactttatgg
     6721 acaaattagt ttcagtttct ttaccaagag ttagagattt tagaggagtt aaccctaatg
     6781 cttttgatgg tagaggaaat tatgctttag gagttaaaga acaattaata ttccctgaaa
     6841 tagaatatga taaaatagat aaagtaagag gaatggatat aatatttgtt actacagcaa
     6901 aaactgacga agaagctcgt gaattattaa aattattagg aatgccattt tctaagtaag
     6961 caaaggaggg attcccgtgg ctagaaaagc gatggttgtt aagcaacaaa gaaagcaaaa
     7021 gtacgctact agagaataca ctagatgtac tatatgcgga agaccgcatt cagttttgaa
     7081 aaaatttggt atatgccgta tatgctttag agaattagct tataaaggtc aaatacctgg
     7141 tgttagaaaa gcaagctggt agaagcttga ttagaatgga aggagggcaa caaaatgaca
     7201 atgacagatc caattgcaga tatgcttact cgtataagaa atgctaatgt tgttaagcat
     7261 gaaactgtag atgttccagc ttctaatatg aagaaagaat tagctagaat cttattagaa
     7321 gaaggtttca taagaggtta tgatgttata gaagatggaa aacaaggtat cataagaata
     7381 caattaaagt acggacaaga aggcgaaaga gttataacag gtcttaagaa aatatctaag
     7441 cctggaatga gagtttacgc tgctaaccat gagataccaa aagtattaaa cggattagga
     7501 atatcagtta tatctacttc aaaaggaata ttaactgaca agcaagctag aaaagaaaat
     7561 gttggtggag aagtaatctg ctacgtttgg taattaatat gaacaatgta aggaggtgcg
     7621 accatgtcaa gaataggtgt taagccaata ataataccag caggtgttga agttactata
     7681 gctgaaggaa atttagttac agtaaaaggt ccaaaaggaa ctttaactaa acaattaagt
     7741 gctgagttaa acataaaaaa agaagaaaat acaataatgg ttgaaagacc aactgataat
     7801 aaaaaacata gatctttaca cggactaact agaactttat tagataatat ggttgtaggt
     7861 gtaaatactg gtttcgaaaa gaaattagaa ttaaaaggtg ttggatatag agctcaaaaa
     7921 caaggaaaga aattagttat gaacttaggt ttctctcatc cagttgagat ggaagatcca
     7981 gaaggaataa ctgttgaagc tccaaaccaa acagagttaa tagtaaaagg tatagacaag
     8041 caattagtag ggaattatgc tgctaagata agagcttgga gaaagccaga gccatacaaa
     8101 ggtaaaggta taaaatacgt tgatgaagta atcagacgta aagaaggaaa aactggtaaa
     8161 aaataatacc aaaacttaaa cgctagaaag gagtgagcta ggtgttaaaa aaggctgata
     8221 aaaatgctaa tagacttcaa agacataaga gagttcgtag aaaaataagc ggtacttctc
     8281 aaagaccaag attatgtgta tttagaagtg ctaacaatat atatgctcaa ataatagatg
     8341 acacaaaaag agtaactcta gttgctgcat cttctttaga agcagaagtt aagagtgctg
     8401 tcaatcatac aggaaacaag gaagcagcta agaaagttgg agaattagtt gctaaaaaag
     8461 ctgttgaaaa aggaatcact gaagttgtat ttgacagagg tggatactta tatcatggaa
     8521 gaattcaaga attagctgaa ggtgctagag aagctggtct taagttctaa tacgaaggag
     8581 ggaaaaatac atgctacgtc gtaagccgat agatgcagga caacttgact tacaagaaaa
     8641 agttgttgaa gtaagacgtg ttactaaggt tgtaaaaggt ggtagaaatt tcagatttgc
     8701 agcattagta gttgttggag atgaaaacgg acacgttggt ataggtgctg ggaaagcaat
     8761 ggaagtacca gacgctataa aaaaagcagt tgaagatgct aagaaaaatc taatagtagt
     8821 gccaatagtt gggactacaa ttcctcacga agtacgcgga cactttggtg ctggaaatat
     8881 attaataatg cctgctgtag aaggtacagg agtaatagct gggggacctg ctagagccgt
     8941 acttgagcta gctggactaa aagacgttag agctaaatct ttagggtcta acaatccaag
     9001 aaatatggta aatgctacaa tagaaggact taattctcta aaaacagttg aagatatagc
     9061 taaacttaga ggtaaaaaag ttgaagaact tctagggtaa ggaggtagtg taaaatggct
     9121 aaattacaaa taaagttagt tagaagtgtt ataggaacta ctcctaacca aaaaaagaat
     9181 gttgaagcgt taggattaag aaaaagagaa caagttgttg taaaagaaga caatgctcaa
     9241 acaagaggta tgataaataa agtaagtcac ttattagaag taactgaaat agctgaataa
     9301 tttttataaa gtagtgcaag gaggtgcaat ccatgaagtt acatgagtta aaacctgctg
     9361 aaggtgcagt aagagctaag agaagattag gtagaggtac tgcaactgga caaggtaaaa
     9421 ctgcaggacg tggacaaaaa ggtcaatggt ctcgttctgg tggtggagta agagtaggat
     9481 ttgaaggtgg acaaatgcca ctagctagaa gacttcctaa gagaggtttc aataacatat
     9541 ttaagaaagt ttacacagaa gttaatgttg aagttttaaa cagatttgaa aatggaacag
     9601 aaataactgc agaattatta aaatctacta aaactataag caaaataggt aaagacggaa
     9661 ttaaaatctt aggtgaaggt aatttagaga aagctttaac tgttaaagct gctaaattta
     9721 cagcttcagc tcaagaaaaa atagaaaaag ctggtggaaa agcagagtta gtataataac
     9781 ctgctaatct cctagccagt acaggcgggg tgaattagcg tgctgtcaaa attaaaacaa
     9841 gcttggaaga ttaaagcagt aagaaaaaaa gtaatgtata cacttatgat gatcgtgata
     9901 ttcagaatag gtactactat accagttcca ggcatagaca ctagtattat acaaaagatg
     9961 gtaggcggca atagtctact ttctctgtat aatatgttta ctggtggagc atttagtaac
    10021 ttttcactat ttgctttagg tataagtcca tatatcactg catcaatcat aatacaactt
    10081 ctaacagtag gttttgaaag tttagcagaa cttcaaaaat ctggtgaaga aggaaagaaa
    10141 aaaatcaata agtacactaa atatacagca ctagctctag cagttgtaca agctttggga
    10201 attacattag gaattgtaag aagtgcttta atatcaaata gtgtgttttt tatcacaact
    10261 gttgtaataa cattagtttc agcaagtatg ttagtaatgt ggattgggga caaaattact
    10321 gaaaaaggaa tcggtaatgg tagttcagta ataatatttg caggtataat atcaagaata
    10381 cctacagatg ttattaaaat atcgcaacaa gttaaatctg gcgaagttgc accttgggtt
    10441 atagtgattc tagctgtagt tatattactt acagtaacag gagttacatt tatacaagaa
    10501 gcaactagaa agatacctgt acaatatgct aaaagagttg ttggaagaaa aatgtatgga
    10561 ggacaaagtt ctcatatacc aatgaaagtt aaccagtcag gagttatgcc tataattttt
    10621 gctagttcac ttttagcttt ccctcaaaca atagctatgt ttatgggtcc aaatgctcag
    10681 gcttttgttc aaaaatactt aagtatggca acagaacaag gattttggac atatagatca
    10741 attgagattc tacttataat tttcttctca tatttttata caactgtatc atttaataca
    10801 gaagacatat ctaaaaatat gaaaaataat ggtggattta taccaggtat aagaccaggt
    10861 gagcctacta tggattattt aaatagaatt ttaactagat taactttagc tggagctact
    10921 ttcttagcaa taattgctat ggtgccagct cttacaacac attatatgaa agtaaatatg
    10981 agtctagctg ggacttcatt gctaatcgtt gttggagttg ctttggagct taagagacag
    11041 ttagagtcaa acttagttat gagaagctat caaggtttct taaaataaaa tggagatgga
    11101 taatatgaga ataatattac ttggacctcc aggtgctggt aaaggtactc aggcggcagg
    11161 aatagtagaa aaatacaata tacctcatat atcaacagga gatatattca gaaagaatat
    11221 aaaagaggga acagaacttg gaaaaaaagc taaagaatac atggaccaag gtttattagt
    11281 accagatgag ttaactgtag gtttagttac tgatagaata tctcaagaag attgtaaaaa
    11341 tggatttatg ttagatggat ttccaagaaa tgtagcacaa ggagaacatt tagatatctt
    11401 cttaaaaaat gctggtatat cactagataa agttgtcaat attgaagttg ataagagtat
    11461 attagtgtct agagcagttg gtagaagaat atgtaagtct tgtggagcta cttaccatgt
    11521 tgagtttaat cctcctaaag tagaaggtgt atgtgatgta tgccaaggag aattatatca
    11581 aagagctgat gataatgaag aaactgtatc taagagaata caagtttatc tagatgaaac
    11641 taagccttta gtagattatt atagcaaaca aggtataata gcagatataa aaggtgatca
    11701 agcaatagat aaagtatttg aagatattgt cgcagcctta ggaagtggaa aataaatgat
    11761 tatcttgaaa tcaaaaaaag aaatagaact tttaagagaa gcaggtaaaa tagttgctga
    11821 cactcatgaa gttttaagaa aagctatttc tccgggaata tctactttag aattagataa
    11881 aatagctgaa gagaatataa gaaaatacaa tgcagagcct tcttttaaag gatatggagg
    11941 ttttccagga agtatatgtg cttctatcaa tagagaggta gtacatggta ttccaggaga
    12001 aactatatta caagaaggtg acattgtaag tttggatata ggagcctatt ataaaggata
    12061 tcatggtgat tctgcgaaaa ctcacggagt aggcatgata tctgaagaag ataggaaatt
    12121 gatagaagta actagagaaa gcttctatga aggaataaaa tttgctaaat taggatatag
    12181 actttcagat atctcacacg cagtacaaac acacgttgag aaacatggtt tctcggtagt
    12241 tagagattta gtgggtcatg gagttggagc aaatcttcat gaagacccac aagtacctaa
    12301 ttatggactc ccaggaaaag gaccaagact tagagaagga atggttattg ctatagagcc
    12361 aatggttaat gcaggtcgtt atcatgtaaa gactctatca gatgggtgga cgactgttac
    12421 aatagatgga aaaaaatcag ctcattatga gcatacgata gcaattactg aacatgaacc
    12481 tttgatattg acaaagttgt aatttaaaaa gtaggtgaaa aaagtgctat cagataattt
    12541 aagtataggt caagttgtta aagtttcatt aggacgagat aaaggaaatt tattctttgt
    12601 agtcaaaata attaataatg aatatgtatt aatagctgat ggaaaaaaga gaaaacttga
    12661 caaacctaag ttaaagaaag tgaagcactt aaaaaaatat gattttatta atgatgaagt
    12721 tagaaaaaga gtagtatctg gacaagagat tacagattct tttttaagag ctgaacttac
    12781 taagttaaat tagataattt aacttattga aatggagggc tggttagtta atggccaaaa
    12841 aagatgttat agaattagaa ggtacagttt cagaagcttt acctaatgct atgttcaaag
    12901 ttaaactaga aaatggacat gagatattat gccacatttc tggaaagcta agaatgaact
    12961 tcataagaat tcttgaaggt gacaaggtga atgttgaact ttctccatat gaccttacaa
    13021 gaggaagaat tacttggcgt aagaagtaga ctatattaat atttagtcta aggagggatt
    13081 aataatgaag gttagaccat cagtaaaacc aatatgtgaa aaatgtaaag taataaaaag
    13141 aaaaggaaaa gtaatggtta tctgtgaaaa tccaaagcat aagcaaaagc aaggataaga
    13201 aattattact agcattttgt taaattttat agtataatac tatattgtga tgttaacaaa
    13261 agaacgttaa aatcgttgaa caatctggcg taggattggc atatgatgtg aagttttaaa
    13321 cactaaaaac tatgtgtagg aggtgcgatc atggcaagaa tagctggggt agatttacct
    13381 agagaaaaaa gagcggagat aggcttaact tatatatatg gtataggtaa agcaactgct
    13441 aacgaaatat tagctaaggc tgagataaat ccagatacta gaataaaaga tttatcagaa
    13501 gatcaagtta atgagttaag aaaagttata gatgacgatt tcttagttga aggtgactta
    13561 agaagagaaa ttgctttaaa cataaagaga ttaagagata taaaatgtta tagaggaata
    13621 agacacgcta aaggtcttcc attaagagga caaagaacta agactaatgc tagaactaga
    13681 aaaggtccta gaaaaacagt atctcgtaaa aagaaaaaat aaataaataa taactagatg
    13741 aaggagggaa aaggaaaatg gctaaaccaa aaaagaaagt tacacgtatt agaagaagag
    13801 aacgtaaaaa tatagaacgt ggtcatgctc atatacaatc aactttcaat aatacaataa
    13861 taactttaac tgacgttcac ggaaatgcta tatcttgggc aagttctgga caattaggat
    13921 tcaaaggatc aagaaaatca actccatttg catctcaaat ggctgctgaa acagctgcaa
    13981 aagctgcgat ggaacacgga ctaaaaagtg ttgaggtatt cgtaaagggg ccaggttcag
    14041 gaagagaagc tgcaataaga gctttacaag caactggact agaagtaact atgataaaag
    14101 acgttactcc aatcccacat aacggatgca gaccaccaaa gagaagaaga gtgtaattaa
    14161 taagagtaca ataggaggtg tagataaatg gcaagatata caggtgcatc atgtagacaa
    14221 tgccgtagag agggaatgaa attattcctt aaaggtgata gatgttatac agacaaatgt
    14281 gctatagtaa aaagaaacta tgctccaggt caacatggcc aaggaagaaa gaaagtttct
    14341 aactatggat tacaattaag agaaaaacaa aaagttaaaa gaatatatgg agttttagag
    14401 actcaattta gaaatttata tgaacgtgct gaaaatatgc ctggtaaagc aggtgagaac
    14461 ttattaagtt tattagagag aagattagat aatgtagttt acagaatggg actagcatct
    14521 tctagaaaag aagctagaca attagtgact catggtcatt tcactttaaa tggaaataaa
    14581 gtagacatac cttcattaat agttaaggtt ggagatgtta tagaagttaa agagaaatca
    14641 agatcttctg caaaattcaa aaatttagta gaagttaact caagaatagc tcctaagtgg
    14701 ttagaagcta atgtagaagg aatgacagca aaagtagttg gtgttccaac aagagaagat
    14761 atagatcttg agatagcaga gcacttaatc atagaacttt actctaaata ataagcacac
    14821 ttttaaaaat tattttaaaa ttttgtttac cctcagtgga ttactgaatt ttaaggaggg
    14881 ttttgtccat gatagaaata gaaaagccaa aagtagatat agttgagctt agcgaagact
    14941 atagatatgg taagtttgtt atagagcctc tagaaagagg atatggaata actataggta
    15001 acgcattaag aagaatatta ttatcatcat taccaggtgt agcagtgaat gctataaaaa
    15061 tagatggagt tcttcatgaa ttttcaacaa tacctggtgt taaagaagat gttactgaga
    15121 taatattaac tttaaaagag ctttcagcaa ctatagatgg tgaaggaagt agaacactta
    15181 aaatagaagc tcaaggacca tgctctatca caggtgcaga tataatctgt cctccagatg
    15241 ttgaaatatt aagtaaagat ttagcaatag ctacattaga tgataatgct aaacttaata
    15301 tggagatatt tgtagataaa ggtagaggtt atgtttctgc tgaagaaaat aaaacagaga
    15361 atgttccaat aggtgtttta cctgtggatt caatatatac tcctgttgag aaggttagtt
    15421 atcatgtgga aaacacaaga gttggtcaaa aaacagatta tgataaatta gtactagaag
    15481 tttggactaa tggtagtata aatcctcaag agggaatatc attagctgca aaggtattag
    15541 ttgagcattt aaacttattt atagacttaa ctgaacatgt aagtagtgtt gaaatcatgg
    15601 tagaaaaaga agaagatcaa aaagaaaaag ttcttgaaat gactatagaa gaattagatt
    15661 tatcagttag atcatataac tgtttaaaga gagcgggaat taatacagtt gaagaattag
    15721 ctaataaatc tgaggatgat atgatgaagg ttagaaacct aggtaagaag tcattagaag
    15781 aggtaataca aaaactagaa gaacttggat tgggattaaa accaagtgaa gaatagcgag
    15841 caaaggaggg ataagaatgg ctaagtaccg taaattagga cgtgaaacag ctcacagaaa
    15901 ccttatgtta agaaacttag taacttgctt actaagaagt ggaagaatag aaactacagt
    15961 gactagagcg aaagaaactc gtagaatggc tgaaaagatg ataactcttg ctaagagagg
    16021 agatcttcat gctagaagac aagttttagc ttatgttatg gatgaaacag tagttaataa
    16081 cttattcaca gatttagctc caaaatatgc tgagagaaat ggtggataca ctagaataat
    16141 aaaaataggg ccaagaaaag gcgatgctgc tgaaatggct tttatagaat tagtatagta
    16201 aggaaaggat taggcttgat ggcttaatct tttttttatt tttttgacta aggtatgata
    16261 taataatgtg taaaatgatt tttataagtt attgcttact aaaaggagta tgagttaatg
    16321 gataatatag taaaggtaaa taatatttcg tttgagtata ttacagatga agcaaaactt
    16381 aaagcaatag ataatttaag cctagatgtt aaaaaaggag aatttgttgc gataatagga
    16441 cataatggtt ctggtaagtc tactttatct aaaaacttaa atgctattct tatgcctact
    16501 gaaggaaata ttcttattga tgatatggat actaaagaag aagaaagatt atgggatata
    16561 agacaaactg caggcatggt atttcaaaat ccagataatc aaatagttgc tactatagta
    16621 gaagaagacg ttgcatttgg acctgagaac ttaggtatag aaccaaaaga aataagacga
    16681 attgtagagg agtctttaaa aagtgtaggt atgtatgatt taagagatag acagcctcat
    16741 ttgttatcag gaggtcaaaa gcaaagagtt gcaatagcag gaattattgc tatgaggcct
    16801 aaatgtataa tatttgatga agcaacagca atgttagatc catctggaag aaaagaagtt
    16861 atgaaaacaa taaaaagact taataaggaa gaaaatataa ctgttataca tataacgcac
    16921 tttatggaag aagcagtaga agctgataga gttgtagtta tggaaaaagg aaagaaaatc
    16981 ttagagggaa ctcctagaga agtattcagt aaaataaaga tgttaaagga aatcggttta
    17041 gatgtacctt gtatgacaga gttatctagt ttattaatag aagaaggtat aaatattagt
    17101 agtgatatat taactgtgga tgagatggtg atggaattat gtcaattata gtaaagaatt
    17161 taacacatat atataatgaa gggatgcctt ttgcaagtaa ggcacttgat gatatttctt
    17221 ttgagattaa agatagagat tttgttggtc ttataggtca tacaggctca ggaaaatcta
    17281 cattaataca acatttaaat ggattactaa agccttcctc aggagaaata tttataaatg
    17341 attttaatat aacagataaa aacttgaatt taactgagat tagaaaaaga gtaggggttg
    17401 tatttcaata tccagaatac caattgtttg aagaaacgat tgataaagat atagcttttg
    17461 gaccctctaa tttaggctta gaggagtctg agatacataa cagggtaaaa gcatcaatgg
    17521 aagctgtagg acttgattat gagggattta aagacaaatc tccttttgaa ttatctgggg
    17581 gacaaaagcg tagagtagca attgctggag ttatagccat gaacccagaa gttctcatat
    17641 tggatgaacc aactgctggt cttgaccctg gtggaagaga tgaaattttt aacttaataa
    17701 aagacttaca tgaaaaaaag aatatgacta taatactatc atcacatagt atggatgata
    17761 tggcaaagtt agcaaaaact ttaattgtta tgaatcatgg tagcgtagaa tttatgggaa
    17821 ctcctagaga agtgtttaaa tcaaatgcta gtaaattaaa agatatagga ttagatattc
    17881 cacaagtttt agagcttgca ttgaaattga gagaaaaagg ctttgacatc agtgaggata
    17941 tattgacttt agaagaagca aaacaggaaa tattaaaagt tgtgagagga cgaggattat
    18001 gttaaaagat ataactatag ggcaatacta cccaacaagt tctgctattc ataaattaga
    18061 tccaaggata aagcttgtag cgacaattgt atttatggta tctatatttg tagtaaataa
    18121 attttggcct tatatagtag ttttattatg tttattagct atgataaaat tggctaatat
    18181 accagtcaaa tatattgtta agggtgttaa acctcttaaa tggattattt tgtttacatt
    18241 tcttataaat atttttttct taccaggtga tgaaatatgg tcatttggat ttttagctat
    18301 tacaaaacag ggtctaagac aagcaatttt tatggcaata agattaatat ttttagtagt
    18361 tggtacatca ttgcttactt taacaacttc accaatagaa ttaactgatg gaatagagag
    18421 attgttgaat ccatttaaga aaattggttt gccagttcat gaacttgcaa tgatgatgac
    18481 aattgcttta cgttttattc ctacattgtt agatgagaca gacaaaataa tgaaggctca
    18541 gatgtctaga ggtgcagatt ttgaaagtaa gaatcttata aatagagcaa agaatttagt
    18601 accacttttg gtacctttat ttgtaagtgc atttagaaga gctgatgaat tggctatggc
    18661 catggaggcc agatgttaca gaggtggaca taataggact aagatgagag agtctgttat
    18721 atcaagaagg gattatatgg catgtgtatt tcaggttgtt tatctgggag caattatagc
    18781 tactagattt atagcaattt aattaataag ttatagatat aatgaggtaa agtatgagaa
    18841 atataaaaat taaaatccaa tacaatggta agaattattg tggatggcaa aagcaacctg
    18901 attctttagg gatacaaggt acaattgaaa gggctatata tgatataaca aaagaagaaa
    18961 ccagtcttat agggtctggt agaactgatt caggagttca tgctattggt cagattgcaa
    19021 attttaaaat taattcaggt ataagtatag aaagtatacc aatggcacta aatgccaagt
    19081 tgccaaaaga catatcagtt atagaagcat gtgaagtaaa tgatgatttt cactccagat
    19141 acagtgcaaa aggtaaaaca tataaatatt tagtatataa tagcaagttt agaaatccaa
    19201 tacttagtga gatatcatat caagtaaaat atgaacttga ttttgataaa atgtgttcag
    19261 aagcaaaaag tttattagga acacatgatt ttaaaggttt tatgagttct ggttcatctg
    19321 taaaagatac agttagaacg atatatgata ttgatataag taaaaaagat gacttgatta
    19381 cttttgagat tagtggtaat ggttttttat ataacatggt gaggattatt gttggaacac
    19441 ttgttgatat gggtcgtggc agaattaacg aaccattttt agatataata caatcaaaaa
    19501 ctaggtcaag atgtggacat acagctccag cacaaggatt atttctcaaa aaagttcatt
    19561 attaacttga cacaccaggc tacatgtatt aaaatatatg agaatgtgtt aataagtcca
    19621 cttgccccgg acttgacata aacggtaaaa aaattttatg aaaagtagtt aaggagggac
    19681 agttatgaaa agttatatag ctaagccagc tgatgtacaa agaaaatggt acttagttga
    19741 tgctgaagga aaaacattag gtcgtttagc aacagaaatt gcgacagtat tgagaggtaa
    19801 acataaacca acatttactc ctcacgttga tggtggagat tttgttgttg tagtaaatgc
    19861 agaaaaaata gttttaagtg gtaagaaatt agatcaaaaa tattacagat accatactgg
    19921 ttatgtaggt ggattaaaag aaatatctta cagagatatg atggataaga aaccagaaga
    19981 agttatatca catgctgtaa gcggtatgtt acctaaaaat aaattaagaa gcagaatgat
    20041 gactagatta agagtatttg caggtgctga gcacactcat gcagctcaaa atccagaagt
    20101 tttaaacttt aaataagatt aataggtgaa aggaggagtt tataatggct aacgttcaat
    20161 attatggaac tggaagaaga aaaagttctg ttgctagagt aagactagtt gcaggtgaag
    20221 gaaatatatt agtaaatgga agagcattag aaaattattt taattatgag acattaataa
    20281 gagatgttaa acaaccatta gttttaactg gtaatgaaaa taaatatgat gttatagtaa
    20341 aagttgaagg tggaggattc actggacaag ctggagctat aagacatgga atatctagag
    20401 ctttattaaa agctgattta gatttaagac ctgctttaaa gaaagaaggt ttcttaacta
    20461 gagatgcaag aatgaaggaa agaaagaaat acggattaaa agcagcaaga agagctccac
    20521 aattctcaaa aagataatta cttatttttt tggaaaagca aaatccttta tttactgtta
    20581 ttcagaaagt attggtattt taaaaatgtt gggttaagtt aattattgat atataataaa
    20641 ataataagat atcttattag gatgtttttc attaagaaga acatcctttt tattttgact
    20701 atttaataaa aaacatatgc taataacatt taaattttat ttttataata ttgtaattta
    20761 aaagtttata taaggttata tattttaaga tattatttaa caatataaat tctgaatata
    20821 cgttatcaaa gtttataata taaactaagt ataatttatt gtatagtatt gaatgttaga
    20881 taaacatagt gatttaaata ggggcagaaa t
//
LOCUS       DAEPOZ010000043        20794 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.043, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000043 DAEPOZ010000000
VERSION     DAEPOZ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20794
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.043"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            78..194
                     /gene="rrf"
                     /locus_tag="KR440_001271"
     rRNA            78..194
                     /gene="rrf"
                     /locus_tag="KR440_001271"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            649..1719
                     /locus_tag="KR440_001272"
     CDS             649..1719
                     /locus_tag="KR440_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0698329.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGKLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
     gene            1703..2572
                     /locus_tag="KR440_001273"
     CDS             1703..2572
                     /locus_tag="KR440_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBH0698330.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            2616..3476
                     /locus_tag="KR440_001274"
     CDS             2616..3476
                     /locus_tag="KR440_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBH0698331.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSMEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLILQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYIPLIIPFMTS
                     SFGIFLLRQNFMQISDETIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            3507..4811
                     /locus_tag="KR440_001275"
     CDS             3507..4811
                     /locus_tag="KR440_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0698332.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INRDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            4874..6136
                     /locus_tag="KR440_001276"
     CDS             4874..6136
                     /locus_tag="KR440_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBH0698333.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKVKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFDLKLDGVEKEAFDFIM"
     gene            6323..8293
                     /gene="uvrB"
                     /locus_tag="KR440_001277"
     CDS             6323..8293
                     /gene="uvrB"
                     /locus_tag="KR440_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBH0698334.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            8312..11137
                     /gene="uvrA"
                     /locus_tag="KR440_001278"
     CDS             8312..11137
                     /gene="uvrA"
                     /locus_tag="KR440_001278"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBH0698335.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            11396..13213
                     /gene="uvrC"
                     /locus_tag="KR440_001279"
     CDS             11396..13213
                     /gene="uvrC"
                     /locus_tag="KR440_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBH0698336.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVEAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            13213..14142
                     /locus_tag="KR440_001280"
     CDS             13213..14142
                     /locus_tag="KR440_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBH0698337.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            14356..16152
                     /locus_tag="KR440_001281"
     CDS             14356..16152
                     /locus_tag="KR440_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HBH0698338.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGFAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENVILE
                     RQRAIKELLSKDDFSIHIQILSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLSPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVISHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(16292..18073)
                     /locus_tag="KR440_001282"
     CDS             complement(16292..18073)
                     /locus_tag="KR440_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBH0698339.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRICMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(18096..19982)
                     /locus_tag="KR440_001283"
     CDS             complement(18096..19982)
                     /locus_tag="KR440_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBH0698340.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            complement(19999..20496)
                     /locus_tag="KR440_001284"
     CDS             complement(19999..20496)
                     /locus_tag="KR440_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HBH0698341.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLTTKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
BASE COUNT         8059 a         2759 c         3551 g         6425 t
ORIGIN      
        1 taactctaaa gaaactagta ttactaggtt ctataaactt attaaaaact taatagaaat
       61 attaagcata aagattatgt ggttacaata gcagagagga tacacctgtt cccattccga
      121 acacagaagt taagctctct agcgctgatg gtacttggtg ggaaactgcc tgggagagta
      181 ggacgtagcc acgtaatttt ttttgggtaa atttataaaa attaaaaaag ttttattttt
      241 tatgtttaaa ccttgtaaat ttgggacata atataattgt aaaacttaat atagcaaaaa
      301 taaattactc aatctccccc acttaaagac ctctatcccc catagaggtc ttttttattt
      361 tcaaaccaaa ttatataaaa aataaatagt tttatatgac tcttcataaa ttaaaatcac
      421 attgtgatag caaaccatga atatgaaaaa tatagaaaat tatagctaac ataagctgta
      481 tatcatctta acttcagtat atttaacaaa aaatgtttat aatttaacct ttattttaat
      541 aagtttacta tatattcact ttgtcttaat atatccttta caaattaaga gtattattaa
      601 tctataagta aatcgtagaa ttaaattaaa tattttggga ggataaaaat ggcagatata
      661 agactagaaa atgtaacaaa atcttataat aaaaaaaata cagttataga aaatttaaat
      721 cttactatag aagatggttc ttttacagta ttggtaggtc caagtggatg tggtaaatct
      781 actacattaa gaatgattgc aggtcttgaa gatataactt caggcaagtt aaaaattgaa
      841 gaaaatgacg ttacaaaaac ggatgctagt aaaagggata tagctatggt atttcaaaat
      901 tatgcattat atccacatat gactgttaga gaaaacatag aatttggtct taaaaacaaa
      961 aaagtagaaa aagaaaaaag agaaaaactt ataacagaag ttattgatgt agtaggattg
     1021 agagaatatt taaatgtgaa accaggcaac ttatctggag gtcaaagaca aagagtggca
     1081 cttgctagag ctatggttaa aaatccaaag gtatttttaa tggatgaacc attgtcaaat
     1141 ttagatgcta aattaagaaa tcaaatgaga acagaattaa tacaattaca tcaaaaacta
     1201 aaatctacat ttgtttatgt aactcatgac caggttgagg caatgtcaat ggcagataag
     1261 atagttataa tgaacaaagg aaagataatg caaataggtt ctccaaagaa gatatatcaa
     1321 aatcctaaga atttatttgt agctcaattt atagggagtc cttgtatgaa catactagat
     1381 ataaaaaatg atattaaatt tggattcaga ccagaaaaag caacactaga gagtataaac
     1441 atagatggaa aaaatgatga tgtatttagc ctatttggaa aagttgtgac taaagaagtg
     1501 ttgggaaatg aaactatata ttgtttatct gttcttaata gagaaataag agtcaaaacg
     1561 gaaaatgata tatttgatat tggaagaact ttaagaataa gtgtattaga aaaagattta
     1621 tattattttg ataaaaatgg agaaagaata gaagatgtaa aagtatgtaa aagtatgtat
     1681 tctaaaatta aaggagatat ttatgaaaaa gcaatttaat aaagcatatt tatatataac
     1741 acctgctatt ataggaactt tattgtttat agtctatcca ataattaaga cgatatctct
     1801 aagcttcaaa tctggtagtc ttcttagttc aacctttgaa aatgtaggct taaacaatta
     1861 tacagaactt ttaaagaatc cagagtttat acaaactata acgaacactg caatttatac
     1921 tttttttatg gtgactatat ctatatcact agccatagta cttgctgttt ggctaaataa
     1981 aaattctata atacacaatt taacgcaaag tataatcttt acaccacatg taatttcact
     2041 agtatctgta gctgtattgt ggctttggat tatggagcca caatatggct ttttaaactg
     2101 gatactatcg acattacatt taccaacatc aaagtggctt tcaagtgaaa gttcagcttt
     2161 aatgtcacta attctaatag gaatatggaa gactcttgga tacaatatct taatagtatt
     2221 atcaggtctt aaatccatac caaactatat atatgaagca tctaaattag ataatgcaaa
     2281 caaaataacg aatttcttta aagtgacatt gccgctatta tcaccaacaa tattttttct
     2341 tatgataaca acaacaactg catcttttca agtatttgat gagataagta taatgacaca
     2401 aggtgggcca gtgggttcaa ctaacatgtt atcttattat atatatgagt ctggatttat
     2461 atactatgac atagggcaag cgagtgcagc gagtatgttt ttgttagcta tagttatgat
     2521 tgctacatat gctaacttta gactcttatc aaagaaagtt cattatcaat aaagaaaaag
     2581 tagatattat aagatttatc caaaaggagg agacaatgga tattatacaa cctatcaaaa
     2641 aagacaaagt tgatataaaa aaaatagcat caaaatcaat ggaaatatta attctattac
     2701 ttttatctgc aatatttata ttccctttta tatggatgct atcgacatcc tttaaaaatc
     2761 cacaagaaat gatgcaatta ccacctacta ttataccaaa tgatatagtg ttagaaaatt
     2821 atagtacagc atggcattca ggtccatttt ttagatattt actaaacagt gtatttgtaa
     2881 caggaagtgt tttaatttta caatttttag ttatggttcc agcagcatat gcattttcta
     2941 agattaaatt taaaggtgaa aaaatgttat ttgggctttt gcttgtagga cttatgattc
     3001 caccacaagt aacttttcta ccagtctata tgatgatgag taagtttgga tttataaata
     3061 cttatattcc acttataatt ccatttatga catcttcatt tgggatattt ctgcttagac
     3121 agaattttat gcagatttca gatgaaacta ttgaagcagc taaactagat aatgcttctg
     3181 acttaaaaat aatgtttaga ataatggttc ctatggcaaa accagcagta ataacattta
     3241 tattatttaa ttttatatac cattggaata attatttctg gcccttagta atgacaaata
     3301 cagatgcaat aagaacttta ccaataggag ttgcgctcct taaatcttca gaaggaatta
     3361 ctgcatggaa tgtaataatg gcaggaaata taattctaat tttaccaata attcttgttt
     3421 atatatttgc gaataaaaaa gtaaaagaag cttttatgta ttcaggaata aaataaaaaa
     3481 ttaaagacta aatttggagg atgaaaatga agcttaaaag aaaattagca acaggattag
     3541 caataacatt attattaggt tctttagttg ggtgttcaaa ttctaatgat gcaaatgcat
     3601 ctaaaggaga aaagactgat agcaatatta cgaagataga tttctgggct cctttaggtg
     3661 gaactaatgg agaaactgct caaaaaatgg ttgaccaatt taatagtgaa cataaggatg
     3721 tacaagtaaa tatgttaaaa cagaaagatt actatgaaaa tgcaacaaaa ctacaggcag
     3781 ccttaacatc taaagaccaa ccagatgtaa cattattaga aataactcaa acaggtacat
     3841 tcgcatcagc aggtgcttta gtagatatga gtaaatattt tgataaaaca tatcaagaaa
     3901 gattcttttc tggtctttta acaaattcat attatgagga taagtttgtt ggtatgccat
     3961 ttaatagaag tacaccaata ttatacataa atagagatat ggcaactaaa gcaggtcttg
     4021 acccttcagg tccaaaatca tgggaagagc ttaaggatta cgctagtaaa atgacaaata
     4081 aatcagaaga tacttatggt tttgaaacac caatagatat ttggttctat gaagctatgg
     4141 taatgcaaag tggaggagaa ataactaatg gtaaaaaagt agcatttaat aatgaggcag
     4201 gacaagcacc agttaaattc tggcaagata tgatgaaagc tggaataatg aaaatgcctc
     4261 ctggagaaga ttataatgcc tgggacgtag caaaacaaga ttttgtaaat ggtaaagtag
     4321 gtatgatatt tacatcaaca gcagatttag caggacttat gcaacaaaca gaaggtaagt
     4381 ttgaaatatc aacagcattc ctaccaaaaa atcaaaaata cgcaactcct acaggtggag
     4441 ctaatctagt tatgcttgaa ggtggaacag ataaagaaaa agaagcaagt gcagaattta
     4501 tagaatggat gacacaaaca gataaaatcg ttcagtttag ttcatcaaca ggatacttac
     4561 caacaactga agatgcaaca aactcagaaa agttacaaac attatataaa gaaaaacctc
     4621 aatataaggt tgcaactgac caattacaat atgctgttgc acttccaatg ttaaatggat
     4681 acaaggaagc aactgataag ctaatggatg agattaaaaa ggggcttact gatttaaatg
     4741 caaatccaaa agatgtagta agcaaaggaa cttctgcaat gcaagcagta atagataaaa
     4801 caaataaata attatataga cttgtaagtt gattaaaagt attcaataat atataaatga
     4861 agaggtaatg taaatgacaa aggttatagt attatcagta gattctttat ttgaaaaaga
     4921 cttggaattt gtaaaaaatc taccaaactt taaatctata ctagaaaatt gctccatagt
     4981 aaaaaatata aattgtgttt atcccactct gacatatccg tgtcatacta gtatggttac
     5041 aggagtatat cctaaaaaac atggtatata tcataatgaa aaatttgacc caaataaaga
     5101 aaataaagac tggtactggt attcaaagga cataaaagta aaaactatta tagatgtagc
     5161 aaaagaaaat aatctaacta cctctagtgt attatggcct gtaatgggag caaatccaaa
     5221 tatagattat aatattgcag aaatatgggc tcctagtaga gaagatgacc caagagaaac
     5281 ttttgaacga agctccagta aggttataat ggacggtatt tacaatagac attgtcatta
     5341 tattgattgg aaattagaac ccaacatgga tttatttgga gtaaactgtt cagtagatat
     5401 aataaaggaa tataaaccag atttaatgct aatacattta gctacattgg accatacacg
     5461 acataattat ggcttattta atgacgaagt agataaagca ttaaaaatga atgataaatg
     5521 gctaggagat attattcagg ctacaaagga tgcaggtaca tacaaagata caaattttat
     5581 aatacttggg gaccatggtc atcttagagt tgataaagtt ataaatccaa atgtcttgct
     5641 taaatcaaat ggatttataa aagttgaaaa tggagaagtt aaggattttg atgcctatgt
     5701 aaattcttct ggaatatcag ctcaaataat agtaaataac ttagataggt taacagaatt
     5761 aagagaactg cttgaagaat ttaaagaaga attattcata gagaatattt ttaccaaaga
     5821 ggaagcatct aaccttggtc ttgaaggtga ttttgaaatg gtgattgaag gtcttgaagg
     5881 aatttcattt ggaaatgatt tcgaaggaag tattataaaa gattcagata taaaagatta
     5941 caagtttgct gttgcgacac atggtcattt acctactaaa ggtaatagac ctccatttat
     6001 agcttttgga ccaaatatta aaggtggtgt ggttatagaa gaaggagacc tgagagcaca
     6061 tgcagcaact atattaaaaa tgtttgattt aaaattagat ggagttgaaa aagaagcatt
     6121 tgattttatt atgtaagttg aataaagtat ttattttcca tgaattatag acttctattg
     6181 caaagataga agtctttttt tatgttttag atttaagtaa aatttaaatg ggtaaaatat
     6241 ttaaaataac ttaaacaatt ttataaaggt atgatataat aatacatgtc aggaactaat
     6301 gttcgaaaaa aggagtgata ctatggattt taaaataaaa tcagacttca aacctacagg
     6361 tgaccaacca gaagctatta aatcaatagt ggattctata aatagaaatg agaaattttc
     6421 gacattatta ggtgtaacgg gttcaggaaa aacattcaca atggcaaata tcattcaaca
     6481 agttaagaaa cctaccctaa taatggctca taataaaact ctagcagcac aactttacag
     6541 tgagtttaaa gaatttttcc cagacaatgc agttgaatac tttgtaagtt actatgatta
     6601 ttatcaacca gaagcatatg tagctcacag tgacacttat atagaaaaag atgcaagcat
     6661 aaatgatgaa atagataaac ttcgacattc tgctacagca tctattttag agagaagaga
     6721 tactataata atatcatctg tatcttgtat atatggactt ggtgacccaa aagactataa
     6781 agaacttatg ttatctataa gaccaggtat gcaaagagac agagatgatg taataaaaag
     6841 gttaatagag atacaatacg aaagaaatga cataaacttt acacgaggta cctttagagt
     6901 tagaggagat atactagaaa tattcccagc tagtaatgat gaaaaagcaa taagaataga
     6961 attttttggt gatgaggtag atagaataac agaaattgat tatgtaacag ggaagattgt
     7021 tggtactaga aatcatgttg ttatattccc agcatctcac tatgtaacaa cgccagaaag
     7081 aattgaaaag gcaattgtag aaatagaaaa cgagcttcaa gaacaaatta aatttttcaa
     7141 ggaaaatgat aggctattag aagcacaaag aattgaacaa agaactaagt atgatataga
     7201 aatgttaaag gaaataggat tttgtcaggg gatagagaac tattctagac atattacagg
     7261 aaggtcagaa ggagaaagac catatacttt aatggatttc ttcccagatg attatttgat
     7321 aattgtagat gaggcacatg tcactatacc acaagtaaga ggtatgtatg caggagacag
     7381 gtctagaaaa acatcactta tagagaatgg ttttagatta ccttctgcac ttgacaatag
     7441 accattaaat tttcaagaat ttgaaggaaa tataaatcaa atgttgtttg taagtgctac
     7501 accaggacct tatgaaattc aacactcaga gacaatagca gaacagataa taagaccaac
     7561 tggcctttta gacccaattg tagaagtaag acctataaat aatcaaatag atgatttggt
     7621 aggagaaata actaaaacta tagaaaaaaa tgaaagagtg cttataacca ctttaactaa
     7681 aaaaatgagt gaggacttga ctaactatct taaagaaata gggataaaag ttaaatattt
     7741 acactcagat atagttacat tagaaagaac agaaataata agagatttaa gattagggaa
     7801 gtttgatgtc cttgtaggaa taaacttact tagagaagga ttggatatac cagaagtttc
     7861 acttatagca attttagatg cagacaaaga aggtttctta cgttccgaaa cagctttaat
     7921 acagactatt ggacgtgcag ctagaaatga aaatggaaga gtaattatgt atgcagacag
     7981 gataactgac tcaatgcaaa atgctattga tgaaacaaag agaagaagag atatacaaaa
     8041 tttatataat gaagagcaca atataattcc taaaactata cagaaaaata taagagatag
     8101 tatagaagct actaaggttg cagaggaaga agttgtatat ggaatatctg atacagatga
     8161 taaagacgaa ataagagcta atatagataa attaaaatca gagatgatgg aagctgctca
     8221 aaacttacaa tttgaaagag cagcagaact tagagataaa gtgaaacaat tagaagaaaa
     8281 attggaaaaa tagaaaggta aaaacaacat tatggaagat aaaattataa taagaggagc
     8341 aaaagaacat aatttaaaaa atatagattt agaattacca agagataaat ttattgtttt
     8401 tacaggtttg tcagggtctg ggaagtcttc tttggcattt gatacaatat atgcagaagg
     8461 gcagagaaga tatgtagaaa gtctatctgc ttatgcaaga caatttttag gtcagatgga
     8521 aaagccaaat gtagaatata tagagggttt atcaccagct atctcaattg accagaagac
     8581 tacctcaaaa aacccacgtt ctacggttgg aacagtaaca gaaatatatg attatttgag
     8641 actattattt gcaagagtag gagatgtgta ttgccctaca tgtggtgaaa aaataagtca
     8701 gatgactatt caagaaatag ttgataaaat gctagatttt ccagatagaa cgaaattaca
     8761 aattctttca ccaattgtta gaggacaaaa aggtacacat aaaaaagtct ttgataatat
     8821 caaaaaagaa ggatttgtaa gagttagagt taatggtgag aactatgaag ttagtgatga
     8881 tataatctta aataaaaatc aaaaacataa tatagaagtt gtagtggaca gaatagtaat
     8941 taaagatgga atagaatcaa gactagcaga ttcaattgaa actgcagtta agctatctga
     9001 tggtcttgta atagcccaaa taatagatgg agaagaaatt cttttttcaa ctaaatttgc
     9061 atgcccagaa catggaattg gcatagaaga attatctcca aggatgtttt catttaatgc
     9121 tccctttggt gcttgtgatg tttgtaatgg tcttggggaa agtaaagaag ttgacccaga
     9181 attagtagta ccaaacaaag atttatcaat aaaacaaggt gcagtggcag catggggttc
     9241 aacaggtgtt aatgatgaca cttattacag taaaatgata aaaagtttag cagagcattt
     9301 taatgtatct ttgacaacac cgtttaaaga tttaccagat gattttgtgg aggaattact
     9361 ttatggtaaa gataatataa ttgtagagtt tacttatgag tcaaaatttg gaggaactag
     9421 aaattataaa tcatactttg aaggtgtaat agtaaactta gagagaagat atagagaaac
     9481 taattcagat tacatgagag ataagattga agaatacatg gcagagagac catgtccaaa
     9541 gtgtaaaggg atgagactta aaaaggaagt tttatctgtt ttagttgatg gaaaaaatat
     9601 aatggaagtt acaaacttat ctgtaaatga attgattgac tttatggaaa atataaatct
     9661 tacagaaaaa cagagattta tagctcatga gattataaaa gagataaaag gaagagctat
     9721 gttcttaaga gatgtaggtc ttgattattt aaatttatca agaaaagctg gaactttatc
     9781 aggaggagaa gcgcaacgta taagattagc tactcaaata ggttctgcat tggttggagt
     9841 tttatatgta cttgatgagc caagtatagg gcttcaccaa agagataatg ataggcttat
     9901 aactactctt agacatctaa cagatttagg aaatacctta atcgttgtag agcatgatga
     9961 agacacaatg agagaagctg attatgttat agacattggt ccaggtgcag gtatacatgg
    10021 tggtgaaata gttgcacagg gtactttaga tgaaataatt gaaaatgaga actcaataac
    10081 gggtcaatat ttaagtggta aaaaacaaat attgcttcct gagactacta gagaaggaaa
    10141 tggaagtttt atagaaatag ttaaagcatc agaaaataat ctgaaaaata ttgatgtaaa
    10201 gttcccatta ggtaagttta catgcataac aggtgtttca ggttctggaa aaagtacatt
    10261 aataaacgat atactttaca aaggtgttgc aagtaaagtg aatagactta agcaaagagc
    10321 tggtaagcat aaggagataa aaggaattga aagtatagat aaagttataa atatagacca
    10381 aagtcctatt ggaagaacac ctcgttcaaa tcctgctact tatacaggtg tatttgattt
    10441 tataagagat atttttgcat ctactaatga agctaaagca agaggttata aaaaaggtag
    10501 atttagtttt aatgtaaaag gtggtagatg tgaagcttgt aagggtgatg gaataataaa
    10561 aatagaaatg catttcttac cagatgtata tgttccttgt gaaatttgta aaggtgaaag
    10621 atacaataga gaaacattgc aagtaaaata caaagataaa actatatctg atatattaga
    10681 tatgaatgtg gaagaagctg ttgagttttt cgaaaatata ccaaatataa aaagaaaatt
    10741 agaaacttta atggatgtag gactttcata tattaaatta ggacagcctt caactcaatt
    10801 atcaggagga gaagcgcaac gtataaaatt agctgctgaa cttagtaaaa gaccaacagg
    10861 aaagactttg tatatattag atgaacctac tactggactt catatggcag atgtagacaa
    10921 gctaatccaa gtattacaaa gactagcaga tacaggaaat acaattatag tcatagaaca
    10981 taatttagat gttataaaga cttgtgatta tgtaatagac ttaggaccag aaggcggaga
    11041 taagggtgga actatagttg caactggaac accaaaagaa gtatctaaag ttgatggttc
    11101 ttatacaggt caattcttaa agaaatattt ttcttaagtt atataaattg tgaaaaattt
    11161 tagagcagat atacgataaa ctgttttgtt ataagtaaat tagtcaaata taagttgtaa
    11221 atagtaatac aaagtaaagt ccatattact cttcatggtt aaaattgttt tcaaatcaat
    11281 tatagaaggg aatatggact ttaacttgtc taaagcactg gaaattgtat aactttttaa
    11341 aattcatatt ttagggtata attttagtat aaaatttaat gttttggagg gcgagatgtt
    11401 tgatatacaa gagcatttaa aaaaattacc ttctgagccc ggagtatatt taatgaaaga
    11461 caaatacgac catataatat atgtgggaaa agccatatct ctaaaaaata gggtaaggca
    11521 gtattttcaa tcttcaaaaa accatacatc taaagtaaaa tctatggtta agaatatata
    11581 taagtttgag tatattataa cggattcaga gttagaggca ttaatacttg aatgtaattt
    11641 aataaaaaga tacagaccaa agtataatgt agtgcttaga gatgataaaa cttatccata
    11701 tataaaagta actacgaatg aagattatcc aagaatctta aaagttagaa gagttcttaa
    11761 agataaggca aagtattttg gaccctatac caatataact gctgtgaatg ataccttgga
    11821 gcttataagt agtacgtatc caataagaag ctgtaaaata gatatagata aagcaataaa
    11881 gaataagaca agaccttgtt taaatctaca cataaataaa tgtttaggac cttgtacagg
    11941 aaatgtttct aaagaagagt atggaaaaat gatagaagag attataatgt gtttatctgg
    12001 taaagaagaa aaattgatgg aacttttaaa ggaaaaaatg aatgagagtt ctatgaactt
    12061 tagatttgaa gaggctgctg tatataggga taagataaag agccttgaag agatgataca
    12121 gaaacaaaaa atagatgcta ctgtaagtga cttaaaccaa gatgtggttg caatggcaag
    12181 agctcataat gaagcttgtg tccaggtatt ttttataaga aatggtaaga ttgtaggtag
    12241 ggaacatttt atattagaag gtgtaatgga tagtccaaga gcttctatat taagttcatt
    12301 tgtaaaacaa ttttataatg aacaagagta tatacctaaa gaattaatta tagaagatga
    12361 gatagaagat agttctatat tagaagaatg gctgtcatct aaaaaggggc aaaaagtaac
    12421 aataagagtt cctcaaaagg gagagaagaa aagcttagtt gaaatggtta ggaaaaatgc
    12481 tgtagaatat cttgaaaaat tttctgatat gaataaaaga aaatatgaaa aaagtattgg
    12541 agcattggaa gaattaaaac aaatattaaa tctagaaaag ttgccaataa gaatagaggc
    12601 ttatgatata tcaaatatac aaggtgtaga ttcgattggt tctatggtag tgtatacgaa
    12661 tgctaaaaaa gataagaagg aatataggag atataaaata aagacagtta ttggacctaa
    12721 tgattatgat tctatggctg aaattgtaga tagaagatta aaacatggaa atttacctga
    12781 cttgatattg ttggatggtg gaaaaggaca agttagtgca gtaaaaaaag tactagaact
    12841 caatgatgta gatatacctc tttggggaat gtacaaagat gataagcata gaactaaagg
    12901 attgatatgc aaagaaaaag aaatagaact agataaaaca actaatttat atagatttat
    12961 tgctagtata caagaagaag ttcataatta tgcaataaca tatcatagaa gtctaagaaa
    13021 taaagctttg acaaaatcta tcttagatga tatacaagga ataggtgaaa aaagaaagaa
    13081 atcattatta aatcacttta aagatgtaga ggctataaaa aaagctacta tggaagaact
    13141 tttagaagta gatggaatga ataaaagcat tgcagataat gtttataatt tttttagaaa
    13201 ggaagaaaat taatggaaac tagcaataaa gtatcaataa gagagataat aaaagagttg
    13261 aagttaaaag tagtatacat gcctgatgat gtagattatt atgtctactc tcaagatata
    13321 aatagaccag gattacagtt tgcaggatat tttgattatt ttgcatatga aagaatacaa
    13381 atagttggaa agacagaata taattttttt ggattaatgg atagtcatat tcgagaagaa
    13441 gtactagata gatttttctc atatgaaata ccagcgttaa taatttctag agacttggaa
    13501 atcaagccag atgtaattga aaaggctaaa aaatataaaa gaatactttt gagcacaaat
    13561 agaaatacca ctagacttat aaatagactg tcaaactatt tggataataa gttagcacca
    13621 cacacaacaa ttcacggggt gctagtagat atttatggta taggtgtact gataaaaggt
    13681 gaaagtagta taggaaagtc ggaaacagca ttagaactga ttcaaagggg taatagatta
    13741 gttgcagatg atgctgtaga aataagaaaa ttagatgaaa gtatgcttgt gggtcagtca
    13801 cctgaactaa taagacattt tcttgagata agaggaatag gaataataga tgttagaagt
    13861 ttatatggag taggggcaat caaaaattct aaaatgatag atttagtagt tcatttagaa
    13921 gcttgggatg aaactaagta ttatgataga ctaggactgg ataaagaata cgaagaaata
    13981 cttggtgtta atgtagagaa attagttgta cctgttaaac ctggaagaaa tactgctatg
    14041 atattagaag tagcagcaat gaattttaga caaagaggaa tgggatacga tgcagctcaa
    14101 gaatttacta aaaaattatc aaaattaatc gatactaagt agtactgaat aatagtgaac
    14161 tacacatcat ttaattaaat attgattagg attatgtcct acaccttata aattaaaatc
    14221 aagttgtata tatataatgt ttattaataa gatatattat ataaggataa tttaaagtac
    14281 agtgttagat tataataata ctagattaat tttatatttg agtttcatta tataacaaga
    14341 aagttggaat tttttatggg aatagaatat tttagtaaac ttttagaaag tagtactgaa
    14401 aaagaattgc tcttaagtaa aaagtcaaat aacataagta cagcaagatt aatatctttt
    14461 ttgatagtga tagcagggtt tgctattgga ttttacaata aaaatatagt aggtattttt
    14521 gttggagttt tatcaataat aatatttata gcactcttag ttattcatag taaagtaaaa
    14581 gaagaagaaa catattttaa aagtaagagc gaagtattaa ataagtatat taagagattt
    14641 ggagatggtt ggaaggaatt taaaatagat ggtaaagaat acttaaaaga tgaaaattcg
    14701 caagcaaaag atttagactt atttggaaga gcatcattgt atcaatatat ctgtgtagct
    14761 aatacatctt atgggaaaaa ttttttagcc aaatatttgt ggaatgaaaa tccagatgag
    14821 aatgtaatct tagaaagaca gagggctata aaagaacttt tatcaaaaga tgatttttct
    14881 attcatattc aaatactcag taatataatt ggtaaagagc aaaaaaataa ttcagatagt
    14941 agtatagaat cgtttataga gtatggagaa aataagaatg tgtatatacc aaaatggatg
    15001 catattttta cttggggtct accaactgca acaatcttat cttttatatt ttgtatgtta
    15061 ggatttttac ctgttttacc tgttttttta ctttttataa tccagttagg gtttagtggt
    15121 tttggatatc caagacttat gcaaactctt tctccattat tttcatttag tagaagtata
    15181 caagtatatg aaaaaatgtt tgaggtttta gaaaaagaga cgtttgagag tgcatatttg
    15241 aaagaattac aagataaact ttctaaggga agtggagttt caagaggtgt taaacaatta
    15301 aattcaatag gaaatgctgt aaacctaagg tataatcaga tactatatat aattgcctgt
    15361 ggtgttttga tgtggaacta tcattgtgca gaagctttgg agcgatggaa aggtatatat
    15421 ggaaatcaaa ttagagattg gtttgaatct attggagaat ttgaagctct aataagttta
    15481 acagttattt ctcatgtaaa ggaaaataca tgttttccag taataaaata tgaagatact
    15541 ccaagattga aagttgaaga ggtataccac ccattaatag cagaaaaaag tgtcatagca
    15601 aattcgatac aattaaattc tcagacttgt attatcacag gttcaaatat gtctggaaag
    15661 acaacatttt taagaagtat cggtgtaaac ttagtgctgg catatgcagg tgctccagta
    15721 tgcgctaaga gttttgatgc gacctgtatg gctatattta cttctatgag aatacaagat
    15781 gatgtaagtc aaggaatatc aactttctat gctgagattc ttagaataaa atcaatgatt
    15841 caatatagtt taaaagaact gccaatgttg gttctagtag atgaaatttt taagggaact
    15901 aactctgctg atagaattat tggagcaagt gaggcagtaa agaaattatc aaaaccctgg
    15961 gttataagta tggtgacaac acatgacttt gagctatgtg acctttcagg tagtggagat
    16021 gttgaagttg taaactatca tttctcagag tactatgtag acgataaaat tcattttgac
    16081 tataagataa aagatggaag atgtaaaact acaaatgcaa aacaacttat gagaatggca
    16141 ggtattcttt aattaataga taggttaatt aaagaataag tctatttaga aatataattt
    16201 aatttgtgaa attaataagg attgtcttaa attacgtaaa agtattttaa gacaatcctt
    16261 ttttaagaat aaattttata ctttgttatg actacttagc ttcttcacaa gcacattctt
    16321 gtggacactc tccatagaca caactcttat caaagtaatg agtatgaagg tatttatggg
    16381 ctacatcact atttggttct ccaaagaagt ctttataaat agcttgtaaa tcaggattct
    16441 catgagattt acgaatagcc ttatttctat ctatttcata taacgcattt gcacgtctat
    16501 caactatttc agtattacca tggtgataag gttgaccagc acctccaaca catccaccag
    16561 gacatgccat aacttctatg atatggtatt tacattctcc acttctgacc ttatccataa
    16621 tctttctagc atttttaagt gtatttacaa tacacacatt aactgtagtt ccatcaacat
    16681 ctatacttgc ttccctaatt cctttaagac ctctaacatt agtgaagttt acattatcta
    16741 aagtcttatt agttatcttc tcatatgaag tacgtagggc agcttctaaa actccaccac
    16801 ttgctccaaa tatagatgca gcacctgtag attttcctag aggattatca aaatcttggt
    16861 cttgaagatt aggtaaatcg atagcagctt cttttatcat tttagctaac tctctagtag
    16921 ttatagaaag gtcaacatct agtattccag attcacttaa ttcttctctt gaagcttcat
    16981 attttttagc cacacaaggc attacagata caacataaag ttcttcagga ttgattccta
    17041 atattttagg tgcatagtag tttttagcta tagaaccaaa cataccttga ggagacttac
    17101 agctagatgc taagtttaac ttgtctggat aattgtgttc taagaagttt acccatgcag
    17161 gacaacaact tgttaatata ggaagatttt cacctttttg gattctttct ataaattcag
    17221 tagcttcttc cattatagta aaatctgcac caaagtttgt atcaaataca tgttcaaatc
    17281 caagtgcttt taaagcagct accatttttc ctgtagagat tgaccctggt tctaatccaa
    17341 actcttcacc aatagcaact cttacagcag gagcaacttg cactactata gtcttttctt
    17401 ttttatttaa aacatcccaa agtttaggta cattatctac ttctgttaat gctccagttg
    17461 gacagacaga tatacattga ccacagaatg tacactctgt ttcaaccata tcagagttaa
    17521 agaatgtact aactaaagta ttaaatccac gattaactcc agataatgct cctactgttt
    17581 gtatgtcatt acatacagtc tcacatcttc tacataatat acacttagaa tggtctttaa
    17641 ctaaagattt agtagaagta tctttacctg caaaagattt ggaaccttga tatcttattt
    17701 ttcttactcc taaatcagca gctatagtct gtaattcaca atctccattt ttttcacaga
    17761 caaaacaatc ttgtggatgg tctgataaca ataattcaac aatagttcta cgagcattta
    17821 atgctttagc actattagtc tgaactttca taccttcctt tataactgtt ccacaagctg
    17881 gaactaagcc tctctctgtc tcaaccatac aaatacgaca agaagcacaa gtgtcaagct
    17941 tatctatttc gttcatatgt aaatgacaaa ggttgtgtat ttttatattt attaatttcg
    18001 cagcatctaa gatagaagtt ccactaggtg ctgaaacgtg tttgccatta atagttaaat
    18061 taactaaact cattgttatt cctcctccaa ataagttatt tcttgattat agcgttaaat
    18121 gtacaattat ctatacaagc accacatttt agacacttag aagtatctat tgtatgttgt
    18181 tccttaacag aacctgttat acaaccagca ggacacactc tagaacattt tgtacaaccc
    18241 ttacagtcat ctgttataaa gtaagaaagt aagttctgac atttaccagc aggacatttc
    18301 ttatctacaa catgagctat atactcatct ttgaaatatt gaagagtact taaaacaggg
    18361 tttggagcag ctttaccaag accacataat gaagcagttt gtatagtttc agcaaggttt
    18421 tctaggtcat ctaaatcttg tagagtacca ttaccttctg taattttagt taatatttct
    18481 aataatcttg tagtacctat acgacaaggt gtacatttac cacaagattc ttcaacagta
    18541 aactcaagga agaacttggc tatatctacc atacagtcag attcatcaag aacaagcata
    18601 ccaccagagc ccatcattga accgatagaa cttaatgaac caaagtctat aggagtgtct
    18661 aaatgttcag ttggtataca tccaccagat ggtccacctg tttgaacagc cttaaatttc
    18721 ttaccttcag ggattccccc accaatttca aatacaatat ctcttaaagt agtacccatt
    18781 ggaacttcaa caagtcctac attttctact tttccaccaa gagcaaagac tttagtacca
    18841 ctagaatctt cagtacctaa gttggcaaac caatctccac ccttaagtat aattgcaggt
    18901 atgttagcaa aagtctcaac gttatttaag caagtaggag atttccatag accactctta
    18961 gaagaactat aagttttcat tctaggttca ccacgtctac cttcaattga atgcattagc
    19021 gctgtacctt caccacaaac gaaagcacct gcaccatatt ttaattctaa tttaaagtta
    19081 aatccagtgc ctaagatatt ttcgccaagt aaaccgtatt tttcagcttg agctatggct
    19141 acttttaatc tttctataga ttttggatat tctgctctta tgtatatgta tcctgtatca
    19201 gaacctatag cgtatccaca tattgccata gcttctagta cactatgagg gtcaccttca
    19261 agtacagatc gatccataaa tgcacctggg tcaccttcat cagcattaca tacaacaaac
    19321 tttttatctg ttggaccagc tggatttttt gaagcagctt cccatttaga gccagtaggg
    19381 aaacctgcac cacctctacc acgaaggcca gaagttttta cctctgcaat aacttcttga
    19441 ggagtaagag aagttaaaca tttgcctaga gctaaataac catcattggc aatatattct
    19501 tctatactat ctgggtctat aagaccacaa ttttttaaag ctatacgtaa ctgttttttg
    19561 tagaaagaca tttcttcttg ttcttcaact tttttactta aagattcttc ctcatataat
    19621 aattcctcga caactgtatt tctaattaag tggctttgaa ctatcttttc agcatcagat
    19681 ggctcaactt ttacgtagaa aacgttatca ggataaactt ttacaatagg accttgagca
    19741 cagaaaccaa aacaacctgt caaacgaaca ctaactttat cttggattcc tgatttcttt
    19801 atttcatttt ctaattcact aactatttct agactgtttg aagaggtaca tccagtatca
    19861 caacaaacaa gtaattctct tcttaataca ttttccttag aataattttt tcttaagcta
    19921 agattaggtt tcagctcatc agctaaaact tttaattcat caaatgaatt tactttacgc
    19981 attatttatt cacccctctt aacaattcaa ttctaattct ctataagtat ctaatatttc
    20041 gcttacttgg tcaggtttca ctttaccata aactttacca tttacagtaa caactggagc
    20101 taaaccacat gcccccaaac atcttaaacc ctctaaagaa aatttaaaat cactagtagt
    20161 ttctccaact tttatgccaa gctgtttttc aaaagcactt aagattttgt cagcaccttt
    20221 tacaaaacat acagttccta gacagacact gattttatac ttacctacag gttctgtagt
    20281 aaaataagaa tagaatgtaa caaccccata tacctttgca ggagctactc caagttttct
    20341 agcaacatga agttgaactt cttttggaag atatccaaat ataccttggg cttcatgtag
    20401 aacttgtatt aacgcacctt cctttgttgt aagagagtca ataaatatat ctagttcgtc
    20461 aaataattgc ttgttgtgtg aaataaaatc gcacataaca aatccccctt ttctttttaa
    20521 agttattatt tttcataaaa taattaacca tgagttgacc ccaacattat gtgggtgaac
    20581 aaaaccagaa caataaagaa agaataatat acaacaattt ctttatttat aaaaacaatg
    20641 caaaaaagtt acctattaaa aataaaaact atactagtct attataatat aaaataattc
    20701 agaatagtta aaaaaaataa aaaaaatgag ctaaattcaa taaaaaaaaa ataatacata
    20761 taaaaaactt taaaaaataa accaaaatgt aaaa
//
LOCUS       DAEPOZ010000044        20645 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.044, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000044 DAEPOZ010000000
VERSION     DAEPOZ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20645
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.044"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            1081..2454
                     /locus_tag="KR440_001285"
     CDS             1081..2454
                     /locus_tag="KR440_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0698342.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILANSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSSRQKKGYEFDDILGESNLIKEAIEKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRRNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDILLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKEN
                     KESNNYSLNDLEGKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
     gene            2710..3777
                     /locus_tag="KR440_001286"
     CDS             2710..3777
                     /locus_tag="KR440_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HBH0698343.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     DVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIIFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMEEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGDVLIEMEHPQQIIPEVE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            3874..4182
                     /locus_tag="KR440_001287"
     CDS             3874..4182
                     /locus_tag="KR440_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HBH0698344.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKVNPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            4175..5605
                     /locus_tag="KR440_001288"
     CDS             4175..5605
                     /locus_tag="KR440_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HBH0698345.1"
                     /translation="MNNSTSIKDMSYQAVMGRNNEIMKNAIGLDYSSFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGAKIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKVIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            5683..6057
                     /locus_tag="KR440_001289"
     CDS             5683..6057
                     /locus_tag="KR440_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HBH0698346.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWNLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            6051..8255
                     /locus_tag="KR440_001290"
     CDS             6051..8255
                     /locus_tag="KR440_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBH0698347.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLRIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            8255..9664
                     /locus_tag="KR440_001291"
     CDS             8255..9664
                     /locus_tag="KR440_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HBH0698348.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELYIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            9649..10713
                     /locus_tag="KR440_001292"
     CDS             9649..10713
                     /locus_tag="KR440_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HBH0698349.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERSIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKKLQDINLPKILIRIPMLSELEEVINYCDISFNSELETI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFNNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            10743..12164
                     /gene="nhaC"
                     /locus_tag="KR440_001293"
     CDS             10743..12164
                     /gene="nhaC"
                     /locus_tag="KR440_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBH0698350.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            12593..13057
                     /locus_tag="KR440_001294"
     CDS             12593..13057
                     /locus_tag="KR440_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBH0698351.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLAKLDVEVGFPVATYIDGKNEILQRIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            13355..13717
                     /locus_tag="KR440_001295"
     CDS             13355..13717
                     /locus_tag="KR440_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH0698352.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            14312..15172
                     /locus_tag="KR440_001296"
     CDS             14312..15172
                     /locus_tag="KR440_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0698353.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIIANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     CVEERDNRQN"
     gene            15430..17544
                     /locus_tag="KR440_001297"
     CDS             15430..17544
                     /locus_tag="KR440_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0698354.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEHDVELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMAEKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHIVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REEYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYVGTLTQLISM"
     gene            complement(17806..20049)
                     /locus_tag="KR440_001298"
     CDS             complement(17806..20049)
                     /locus_tag="KR440_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBH0698355.1"
                     /translation="MSNIIIKGLTQNNLKNISFKIPKNKIVVFTGVSGSGKSSIVFDT
                     IAAESQRQMNETYSSFIRNRLPKYRKPQVELIDNLTASVIVDQTPLGGNARSTVGTIS
                     DLYSSLRLLFSRIGEPYIGTTSYFSFNDPNGMCKNCSGIGRVKVINIESIINPENSWN
                     TGCINDSLFKPNSWYWKQYAESGLFDLDKPIKDYSFEEYNLLLYGSKNKDGKQENPKI
                     EGIFNKYNQTYLNRDISNMSKHTKEKSERLITDKECPVCHGKRLNKETLNCNILGYSI
                     ADMCNMELTDLYDLLLQIKDKRTETVIKTLTDGLKRMIDIGLPYLHLNRESSSLSGGE
                     AQRLKLVRYMGSSLTGMTYIFDEPSAGMHPRDVHRMNQLLKKLRDRGNTVLVVEHDKD
                     VISIADEVIDIGPSAGTQGGHVVFQGSYDKLLLANTLTSNAMNLSLPIKTDSRHPKGT
                     LPIRDACINNLKHIDVDLPVGIMTVVTGVAGSGKSTLISRVFAQKYEKDVIKIDQSPV
                     TATNRSMPATFLGFFDEIRKVFAKENNVEEGLFSFNSKGACPVCDGKGVLVTELVFMD
                     PIINTCEACKGTRYSEEVLSKLYKGKCIVDVLSMTVDESLEFFTEKKLCKILQTMKDV
                     GLPYMTLGQPLSTLSGGERQRIKLAKNLNKKGTIYILDEPTTGLHGSDIEKLMLIFEH
                     IVDKGNTVIIIEHNLDVLKQADWVIDIGPDGGKNGGQVVFEGTPLEMFERGCTITSDF
                     LRKSINI"
     gene            20381..>20645
                     /locus_tag="KR440_001299"
     CDS             20381..>20645
                     /locus_tag="KR440_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698356.1"
                     /translation="MILIVILSIFIIASINIIILGKENIRDIVIPLTLFIVSDFLFLC
                     GNIICRGGVMLLFIGLFAIPLMLIAFIWICVHMIKLYYKKDLNK"
BASE COUNT         8027 a         2389 c         3630 g         6599 t
ORIGIN      
        1 agacagtttt acatatttca actgtctcct aagaggggta tagttcacaa ctaagagtgg
       61 ctgggaactt aatataaagt tccagaaaag taaatcttca aaataattta ttgaaggaaa
      121 catcactcaa agttaagtaa aatatgtttc cttgctttta ttatatcaca aattaaaaat
      181 attaacacaa aattttgtga atatatactt attattgttc taaaaaaata tgtaaataat
      241 tgatttgatt tcatagaatt attgttgtaa tatttagtta aatatacaca atctcaatta
      301 aagaagataa gtattataaa ttcatatcaa aaaaatacat aaacttttaa gttataattt
      361 tataaaaagt cttttagtat cattatttca agaggaatta acatatgaaa aaataacttc
      421 attagaaaag tggataaaaa actggtaaga agatgactaa agattacata tactattact
      481 taaattaaaa atttaatata atttataatg tatacaattt attataaaaa atttaatttt
      541 aattttctat ttttgatact ataatatata atcttgataa tttaattaaa aatctagatt
      601 ttacatacca aactggtttc ttatttcgct atctttactt atgaactcat atattcctat
      661 aaaggttatt ttagaaaaag ttggttattt aaacattaat accacaattt ctatagattg
      721 tttgttgaat aatctataat tatattatgg actaaaaaat ccaatgagtt aatttattgt
      781 ttttttatat ttgagagatt acaacttatt agtttgtata tctctttttt taataaaatc
      841 aataatatat aaaactattg gcatatttaa tgacaattca aatattgtat ataaccataa
      901 tgaaagatat ataaataaaa ttttaaatat acttaaaaat atattaatat aatgtacata
      961 aaaaatatca taaatatatg ataagataaa tacaatagca atattttatt aattttttgg
     1021 gtttttatat atttaggtac tggggtgata tagttgaacc aagagatatt ttttaaaata
     1081 atgaatgaaa tattggaaaa tattgatgaa ggggtacatt ttgttaataa agacgggaaa
     1141 actataatct acaataattc tgtacaaaaa atggaaaaaa tgaacagaca cgatatttta
     1201 gccaattcat tttttgatat agtcaacaaa atgaaaataa acaggagtac actattagaa
     1261 gttttggaaa aaagggaagt tatcaaagac aatattcaaa aatatctgga caaaaatgga
     1321 aaagagataa cagcaattaa tactacaata ccaattgata ttaaaggcga gtttgtaggg
     1381 gcacttgaaa tatcaaagaa tatgacaacg atagaaaatt tactaaacag aaattataaa
     1441 aaaaatttag tcaataaagt tagtagtaga caaaaaaagg gttatgaatt tgatgatatt
     1501 ttaggggaaa gtaatttgat taaagaagca atagaaaaat ctaagaaagc ttgcaagtct
     1561 gatgcttcag tatttatata cggtgaaact ggaacgggta aagagttatt tagccagtcc
     1621 attcattatg gaagtaatag gagaaactat ccatttatag cacaaaattg tgcggctttg
     1681 ccggaatcac tattcgaagg tatccttttt ggaactagca agggaggatt tacaggagca
     1741 atagataggc caggcttatt tgaacaagct aacaaaggga cattgttatt ggatgagata
     1801 aattccatgc ctattatgtt acaggctaaa ctattaaggg ttttacaaga aggttatgta
     1861 agaagggttg gaggaactaa ggatatacct atagatgtaa gaattattgc aacgacaaat
     1921 gaaagtccaa aggtaatttt aaacgagcat aaaatgcgaa aagatttata ttataggctt
     1981 aacataattc atataaaaat tcctcctctt agagatagaa aagaggacat acttctattg
     2041 tgcaaaaaat ttatttcaaa gtataataaa aagttaaata aaaaagtggt aggaatttca
     2101 gatgaagcta ttaaaatttt aaaaaattac gattggcaag gtaacataag agagctagaa
     2161 aacataattt attgtactat gagtatgatg gaagatgagg tacaaataga aacaagtatg
     2221 attaatctaa acgactatta caatcttaaa gagaataaag agagtaacaa ttattcttta
     2281 aacgatttgg agggcaaaac acttagtaag attatcagtg agatagaaga aaagtatata
     2341 tatgaatcat tagaaaaggc atcgtacaat ataactaaag ctgcatcaat tttaggtatg
     2401 aataggcaaa atttacagta taaaataaaa aaatataata tcaaaatatt ataaataagc
     2461 aagataaaaa aacaaaatag aagtaaaata aaaaagcaaa atataggagc aaaattatta
     2521 taaaagagca aaatattttg ctcttttttt tatttctcat aagaaatatt attttttatt
     2581 tatgaaaata cataataaaa taattatagt ttcaacgttc caataaatat ttactttaat
     2641 tttaaagttg gcacgattta tgctttaact aattattgag aatatataaa tttttaggag
     2701 ggtgtatata tgagaaaagt aagagtagga atatggggat ttggagctat gggaataggt
     2761 atggctaata tgattctaaa gaaagaaggt atagaaatag tatctgtttg tagtagaagt
     2821 acttcaggga aaagtatgta tgatgtttta ggaattgaaa gaggagaaag accagaagtt
     2881 attatcaata aaaattatga agaagttttt agagaaaaaa gtgtagatgt tgttttgtta
     2941 gcaactgatt catttactaa aaaggctttt gataaaataa ttttcttatt aaatagaaaa
     3001 ataaatgtta tatcaacagc agaacaaatg gcatatccac aagctgatga tgcagattta
     3061 gctaaaaaaa tggatgaagt tgcaaaggaa aatggagtaa gtatacttgg aacaggaata
     3121 aatccaggat ttgtattaga tttactagta ttggcacttt caggtacttg tgaagaagtt
     3181 actagtataa aagctaagag agtaaatgat ttatctccat ttggaaaatc tgttatggag
     3241 gaacaaggtg ttggagtaac aagggaagaa tttataaaag gtgtagaaga taagactata
     3301 gcaggtcatg taggttttgt agaatcaata aatatgatag ctgatggatt aggttggaaa
     3361 ttagataaga tagaacaaac aaaagagcca ataatgacta cagtagatag aaaatctaaa
     3421 tacggagagg cattggctgg aaatgttgct ggttgtagac aatgtggata tggatatgta
     3481 aatggagacg ttttaattga aatggagcat ccacaacaga taataccaga agtagaaggt
     3541 attaaaacag gagactatgt atcaataaaa ggtataccaa acattgattt acaaataaat
     3601 cctgaaatac ctggaggagt aggtacttat gcaatgatag taaactctat tccattaata
     3661 ataaatgcta gaccaggtct taaaactatg ttggatatac cagtgcctag agcaataatg
     3721 ggagacataa gaaatcagat agaagttgaa ttggaagaag aatcaaaggc taattagtaa
     3781 gataacagtt agctaattgg taaaataatg tttaatttaa aataacagta aattaattag
     3841 taaggaatag tcaattaata aggggatgaa aaaatggatg ctaaaattaa tgattgggtt
     3901 attatacaca atatagtatt gactccagaa gaaagagctc cacaagttcc agaggatact
     3961 aagaaagtat ccttagaaat gtgggttaaa ggttttatac aaagtgatgc ttctataggc
     4021 gacttagtag aagttaaaac aattacaggt agacttgtaa aaggtaattt acttaaagta
     4081 aatccatatt atactcatga ttatggtaaa tgtataccag agttactcca aataggaatc
     4141 caagctaaag aaatattatt tggaggtgta tataatgaat aattcaacat caataaaaga
     4201 tatgagttat caagctgtta tgggaagaaa taacgaaata atgaaaaatg caatcggatt
     4261 ggattattct tcttttgaac aagagggaat agggtttgat tatgagaaaa tgatgagtga
     4321 aacaggatat acattacaag atattgaagc aattcaatca cagtatgctg ttggaaatac
     4381 tcctctaata gagcttaaaa atttgactaa attagctaga aaatgtgcta aagaaggtaa
     4441 aggagctaaa atatttgtaa aagatgaagc aatgaatgca tctggtagtt ttaaagcaag
     4501 aagagcagct actgcagtat atcatgccaa acaaatggga tataaaggtg taatagcagc
     4561 taccagtggt aactatggtg cagctgtagc atcacaagcg gcaatgcagg gattaaaatg
     4621 tataatagtt caagaatgtt atgattctaa tggtgtaggt caacctgaaa ttatagaaaa
     4681 agcacgaaaa tgtgaggcat atggagcaga agttgttcag ttaactgttg gaccagagtt
     4741 attttatacg ttcttagttt tattagaaga aacaggctat tttaacgcat cattatattc
     4801 accatttgga attgcaggtg tagaaacttt aggttatgag ttagctatcc aatttagaga
     4861 aaagtataaa aaagaccctg atatagttgt ttgtacaaat gcaggtggtg gtaatcttac
     4921 tggaactgca agaggattga taaaagctgg ttccattaat actaaagtag taggagcaag
     4981 tgtagattta aaaggattac acatggctag tgataatcaa tttaataaaa aatcatttac
     5041 aacaggacat acaggttttg ggataccata ctgtacatgg cctgataggt cagatgtacc
     5101 taggtctgca gctagaccac ttagatatat ggatagatat gtattagtaa aacaaggaga
     5161 agttttttat acaactgaat tattggcaca gctagaaggt atagaaagag gtccagcagg
     5221 aaatacatct ttagcagcag cattctcttt agctcaagag ttagatgagg ataaagttat
     5281 agtggtacaa gaaacagaat atacaggcgc aggtaaacat atatgtccac agcttacttt
     5341 tgcaagagaa aatggaatag atataaaatt tggaaatcca agagaagaaa ttgcaggagt
     5401 aaacttaata ttaccagaaa gaccagaatt attgaaatgt gttgatattg atatgaataa
     5461 aataagaaag tcatttataa aaaattgcat atctaataat cacattgata atattgataa
     5521 gctaagtaat cgagatatag aatttttaat ggaagaagta aaaagttcaa gagattttgt
     5581 tgttgatgta ttaaataatt tatagtaatt attgtttttt tatttaatat aaaataaaaa
     5641 ttcggaaaat tctattaaat caatgcgaat ggaggaatgt tcatgaaaaa aagagaggat
     5701 gattttgaag ttagaagaaa acatcttcaa gaactgagtg atgatgagtt aaaagaaagg
     5761 ttttggaatt tagctaccca gatagtagaa ccaatgatag aacttggtaa aaagaataca
     5821 acaccatcaa tagaaaggtc tgtcttgttg agaatgggat tttcttctct tgaagtaaaa
     5881 cctattttag aaggggttat ggaaagaggt cttataggta aaggagcagg gcatgtagtt
     5941 tataaattgg ctaaatctaa aaatataacg gttagagaag caggtttgtt attagttaag
     6001 ggtgaatatt gggatgaagt tacatgttta tttagagaag gggtagaatc atgttaaagg
     6061 aaaataaaaa attggatata gactatatat taaaagattt ggataaatat aaaccaaaac
     6121 gaagaggttg ggtttggaga gaacacttgg aaaatcttga aatgggacct tttaaatata
     6181 aagactgtac aaaaccttta aagaagagtg tagggctacc atcatctaag tatttcaata
     6241 atatagaccc tcaaccaagt ccagttatta ctactgaaat agcttctggt agatttgagg
     6301 atgatataag aagaatgaga atggcagctc atcatggagc tgaccatcta atggttatca
     6361 gaactgctgg acaatcacat tttgatgggc ttatagaagg aactccacaa ggtataggtg
     6421 gagttccaat aacaagaaaa caagtaagag ctcaaaggaa ggctcttgat tttatagaag
     6481 aagaagttgg tagacctatt aattaccatt catatgtaag tggtgttgct ggtcctgaag
     6541 tagcagtaat gtttgctgag gaaggagtca atggagcaca tcaagaccca caatataatg
     6601 ttctgtatag gaatataaac atggtaaggt cttttgtaga tgcttgtgag gcaaagaaaa
     6661 taatggcttt tgccaatata gctcaaattg atggagcaca taatgctaat gctactgcta
     6721 gagaagcatg gaaggtaatg cctgaactta tggttcaaca cgctttaaac tctatattct
     6781 cagaaaaaat aggtatagat aaaagtaata tatgcttatc aacagttcca ccaacagcac
     6841 ctccagcacc atgtttaaga atagacttac cttacgctgt tgcacttaga gaattattta
     6901 gtgattataa gatgagagct caaatgaata ctaaatatat ggaatcatca actagagaag
     6961 caacagttac tcatgtatta aacttattaa catcagtatt aactagagct gacatacaat
     7021 caactataac acctgatgaa ggtagaaatg taccttggca tatatacaat atagaagcat
     7081 gtgatacagc aaagcaagct ttagttggta tggatgggct tatggatatg atagaactta
     7141 aagatgtcgg tgaattaaga gacaaagcaa gagaacttaa agaaagagct gtattatata
     7201 tggaagaaat atctgaagtt ggtggatatt ttgaatctgt ggaacaagga ttttttgttg
     7261 attctggaaa ttatccagaa agaaatggtg atggaatatc tagaaaaata aaaggtggag
     7321 ttggtgaagg tactgtttat gaaagagaag aagattattt agcacctgta actgctcact
     7381 ttggatacaa taatgtagct caatatgatg aaaatgctat agataatcca tcaatgttaa
     7441 tagatggatg tacatttgaa aatccagaca aaataatata tattgatgag cttgacgatt
     7501 ttgataatgt agaaaataga ctaaaagaat catatgagta cagaaatgga actaaaatta
     7561 agcctgaaat ggaatggtgt gcagatggaa ttgtaatgat aacaatgatg cttcctactg
     7621 ataaaagaac agcagagttt gcagctttgg aatttgtcaa aaaaatgaac ttacaagaaa
     7681 ttgaggtcat aagtagagaa gttatgcatg aatctgaagg gactagaata gaagtgaaag
     7741 gaagagttcc gtttgatata gatttaaaca atttagtaat accagaagaa cctaaggtac
     7801 taactgatga agaaataaga gcagatatag aagaaaaacc tatgaaaata gtttcagcta
     7861 cggttggaga agatgaacat tcagttggtt taagagaaat aatagatata aaacatggag
     7921 gtattgaaaa atatggcata gaatgccact atctagggac ttctgtacct gtagaaaaat
     7981 tggtagatgc agcgattgaa ctgaatgcag atgcaatact tgcatctaca ataataagtc
     8041 atgatgatat tcactataaa aacatgaaaa aattacacga ttattgtgta gaaaaaggta
     8101 taagagataa agtaatgatt gcgtgtggag gaacacaagt aacacctgaa atagctgttg
     8161 aacaaggcat cgatgctgga tttggaagaa acagcaaagg catacatgta gccactttct
     8221 tagttgaaaa aagaagagaa atgagtaata aataatggaa aatagcaaag tttctttaaa
     8281 aatagatgtg ttggtagctg aaattggaag tactactaca gtggtaaacg ctttccacga
     8341 tattaatact aacaatccta tatttttggg tcaaggtcaa gctccaacaa cagtgtttga
     8401 aggtggcgat gtaagaaatg gcttatcagg tgctattaaa gatttagcta ataaattatc
     8461 tgtagatgat atagagtata atgatatgtt tgcaacttct agtgcagctg gtggattaaa
     8521 aatgactgtt catggattag tctatgatat gacagtaaaa gcagctaaag aagcagcttt
     8581 aggggctgga gctgttattc gtcaaataac atcaggtaga attaaaagaa ctgatttaaa
     8641 taaaataaaa gaaataaacc caaacataat tttaatagct ggtggagtcg attatggaga
     8701 aagagataca gctatttata atgcagaaat gatagcttct atgaatctag gcataccagt
     8761 tatatatgct ggtaatgttg aaaatcaaga ggaaataagg ttaatatttg aagatactaa
     8821 ttataagctt tatataactg aaaatgtata ccctaaaata gatttattga atatagaacc
     8881 tacaagaaga atcattcaaa gtgtatttga agaacacata actacagcac caggaatgaa
     8941 atacattaaa gaaatggtta atgaaaatat tactccaacc ccaggagctg taatggaagc
     9001 ttctaaactt ttatataaaa atattggaga tttattaact ttggatgtag gtggagcaac
     9061 tactgatgta cattctgtta cagatggaag tgactatatt aataaaattt tggttaatcc
     9121 tgaacctact gcaaagagaa gtgttgaggg ggatttaggt gtatatgtaa atatgaagaa
     9181 tatagtagaa gttataggta aagaaaactt acaaagtgaa ctatatatag atattgatgc
     9241 agtaatagag aattatcctc caataccaaa atcaaaagaa gaaatattat ttgttgagag
     9301 gttgacaaaa gaagctgttg ttaaagctat gctaagacat tcaggaaaaa taagaaatat
     9361 ctatggaaca actggaaaag tcaaaattgc agaagggaaa gatttaacag aagtaagata
     9421 tatagttggt acagggggag cacttactag gctccctagt agaattgaaa tattagataa
     9481 tatgctcaaa tacaataaaa ataatgaatt actatttcca aaggaaaaaa caaagatatt
     9541 aatagataat gactatatta tggcatctat gggtgtttta tctaaaaaat atgaagaagc
     9601 atctcttaag ctcctgttaa aaagtttgaa ttttgaggag gaaagattat gtatcctagg
     9661 ttagaaatag atatagaaaa gttaaagcat aatgcaaaat taatttcgca gatgtgtcat
     9721 gaaagaagta ttaagatatc ttttgttact aaatcatttt gtgcacaaaa agaaatagta
     9781 gaagaaatat gtagtgaagg aatagaccat atagcagatt caagaataca aaacttaaaa
     9841 aaattacaag atataaatct acctaagata ttgattagaa tcccaatgtt aagtgaatta
     9901 gaagaagtta tcaattattg cgatattagt tttaactcag aattagaaac aataaagaaa
     9961 ttaaatgaat tatgtgaaag taaaaatatt attcataaga tagttcttat gtttgactta
    10021 ggcgatttac gagaaggata cttttatgaa gaagactttt ttaataatgt aagagagata
    10081 gtaagattaa aaaatattga aataattggt atagctacaa acttaacctg ttatggagct
    10141 attataccat ctagggaaaa tataggcaga ctggtaagta tagcaaaaag aatggaagaa
    10201 aaatttggaa taaatctaga aatagtatct ggaggtaatt caagttctat tcatctgttg
    10261 ataaataata atatgccaga aggtattact aatttgagaa taggcgagtc cattctactt
    10321 ggtagagaaa cggcatacgg tgaaaatata aatggaacat atcaagatgc atttaaatta
    10381 atatgccaag tagttgaatg taaagaaaaa ccatcagttc ctattggaga aataggagtt
    10441 gatgccttta gaaataaacc tgtttatgaa gataagggaa tcttaaaaag ggcaatcata
    10501 gctataggta aacaagatat aaatattgac tctttaattc caatagacac tgatataaaa
    10561 atattagggg caagttcaga ccacatgata ctagatgtaa gtaacacaaa atatgattat
    10621 aaattaggtg acaatttaga atttttatta acttatgggg gtatcatgtc atctagtaca
    10681 agcaaatatg tttcaaaaaa aattatagtg tagtgtttaa ataattagga ggaaactatt
    10741 ttatgaaaga gcgagaatgt agaaaagcaa gtctgtttga tgcaatgata ccaattactt
    10801 gtttgatatt atttttaagt ctaggagtat tagtgtatgg ttcatcacct catgtaccac
    10861 taataggagc agcagcagta gcaggacttg tggctgtata tagacttgga tttaaatgga
    10921 aagagttaga attaagtatg tttaatagta taaagatggc aatgcaagca atcttaatca
    10981 taattataat aggcgtactt ataggaactt ggatagtaag tggagtagtt ccatgtatga
    11041 tttactgggg actaaaaatt ttatcaccaa atattttttt agtagcatca acattagtct
    11101 gtgcaatagt atctcttgcg acaggttcat cttggagcac aatgggtaca gtaggagtag
    11161 ctcttttagg aattggtcaa agtttaggta tgccaattgg attaatcgta ggttctataa
    11221 tatctggagc ttactttgga gataaactat cacctttatc tgaaacgact aacttagctc
    11281 cagcaatggc aggaacagat ttgtttacac atataaaata tatgttatat tcgactatac
    11341 catcattatt aatttgttta gtaatttatg gtgtaattgg aatgaagtat tcaggtcaag
    11401 cactagatat aaaacaaata gaattaatta ggtctacatt agattctaca tttaatactt
    11461 tatcaccagt attattatta gctccagcaa tagtaattgg attagttgta tttaaagttc
    11521 ctgctatacc agggcttata gtaggtgctg tattgggagt attgtttgca gtagtttttc
    11581 aaggagaatc tatgaatacg atattagatg cagcacaaaa tggatatgtt tcgtcaacac
    11641 aaatacagga ggtagatgct ttattatcta aaggtgggat tatgaatatg atgtccactg
    11701 tatccttaac aatatgcgct ttatctcttg gaggtatttt agaaaaaaca ggtatgcttg
    11761 aagtagtagc atcttcttta ctaagattag caaagggtgt atttggaaca gtattatgta
    11821 ctatggtaac ttgtacaatt accaatataa tagcaggtga acaatatcta tctatagtta
    11881 taccaggaag aatgtataat aaggaatata aaaagagagg tatacatcct aagatgttgt
    11941 cgagagcttt agaagatagc ggaactttaa cttctccatt agtaccatgg aatacatgcg
    12001 gggcgtatat aacagcaaca ttaggtgtat ctgcactaac atatggtcca tacgctttat
    12061 taaatattat aaatccaata gtatctttag tattaattgc atgtaagttt aaaatagcaa
    12121 gaatagaaga tgaacctgaa acttgtttaa attttgatgt ttgataatag ataacttatg
    12181 taagaacgta ggaactaggg ggggagcctg tggggaatta gtaagacata ttttagtgtg
    12241 cgaaattcta taaatcagta atcttgtgac tttagccatg ggaggttcag attagtaaat
    12301 ttatagtttt atattagtct atactttgat cttttaattg tttgtataaa ttttttatag
    12361 aaaaagattt cttttagact tgcctaaaaa ttaggcaagt cttttaagta tgttctgatt
    12421 ataagagcac tgtgtgacaa gaatgatgag attatttttg ctatactttc gatataagaa
    12481 atggaaggag gttatgtttt atatttatat gaaataatta gttacacagt gaattttcgt
    12541 ataatttggt ataaaaagat tagaaatgaa aataaagtaa tggaggaata aaatgactag
    12601 aatatccaat ataacaatag ctgaacaatc tgagtactgt tttttggcaa tacgaaaaac
    12661 aattgacttt atggtagagt tttcagagtt ttcaaaacaa agttttgaaa aaatttctaa
    12721 atatctggag gagagaggaa ttttatcttc aggagcaccg attgtatgtt ttcataacat
    12781 ggatttagca aaattggatg ttgaagtagg gtttcctgta gccacttata tagatgggaa
    12841 aaatgagatt ctacaaagga ttgttcctgc acagaaaata ataactgcaa ttgatttagg
    12901 accttatgaa ttacaagacc ctactttaga agacttattt tcatgggcaa atagcaatgg
    12961 atataaactt catggcgata tatattacca gtatttaaat gatatcaatc gtccagctag
    13021 tgaatatctt acgaaaatga tgttacccat tatatagaaa gttaaagatt atcgtttgtt
    13081 agttgataat tagaagaaca tttaatgcta ctgcataaat tttatcctaa ataatatcaa
    13141 ggtgatttaa tttatgtatt ctaatgtaac tgtagataat caatttataa ctaacttagt
    13201 gtaatttaga tttaatttat tattctaatg taaccccaat atattgttaa ggtctactta
    13261 agatgctcaa aaggctatgc ttaatttatt gttatataat ttgtagagtg gtataattta
    13321 gaagtgatac tatatagata tataggggga aaatatgaaa atatatgata tattaacacg
    13381 tttttatatt caagatatag aaaaagccat acctttttat gagaatcttt taaaagaaaa
    13441 atgttctttg cgtttttctt ataaagaagt tgggttggaa ttagcgcaaa taggaaatgt
    13501 tttactatta tctggttctg atgatgctct taaaccattt atagaaacaa aatctacttt
    13561 tatggttgat tctgttgatg aatggagaag ttatctattg gataatggag cagttgtagt
    13621 aagagataag aaaaaagttc ctacaggata taatatgaca ttgagacatc cagatggtac
    13681 aattatagaa tatgttcaac atacaaagca ggattagtta aaaatagatt ttactactga
    13741 gcaactaatt agaatctatt cttagacgta caatttatta tttttggtat taatagatta
    13801 taagtcaaat tataatattt caaaatagaa tagaaattta tttacataaa agagagtgtt
    13861 taaaaatgaa ctttttagtt cacgaagcac tcctttttgt atgaaatcaa atacatcaat
    13921 atataaaaca aactgatgaa gagcttaaat taaaataaaa agattcataa caggacccca
    13981 ttaaacttta agatagggta agtttgtaat catattaaat aattgtaaaa agctgttttt
    14041 agtcgtataa tataaatatt tatcatttaa ttaaggaatc taaaaggaat ctaataagaa
    14101 gaaaataata aataactttt tagatttact aaatgaaaaa ttattgaaaa ttaaaggatt
    14161 agagtcttta atcagaaagt aaaaagtaat atattaaatt tatgaattga aaacttaaac
    14221 aacaaatttg tttataaaat agcaagattc attaaatata actaagtaat aattgatata
    14281 atgttgcatg gataaaagga gggaagctgc aatgtcttac aaagtaatta atgtcataac
    14341 tgctattgac tatatagagg agcatttatc agaaaagctg gatttagata taattgctaa
    14401 tgctgttcat tactcaaaat atcacctgca tagaactttc acaacttctg ttggtctaac
    14461 aatgcatgat tacataaagc ggagaaagtt gacagaagca gcaaaattat tggtattttc
    14521 aaagaaacca attattgaaa ttgcattgat agctggatat gaaagtcaac aggcattcac
    14581 ttctatattt aaagctatgt ataaaaaatc tccaaataaa tataggaaag aacaagaatt
    14641 ttatcctttg cagctaagat ttgttttgaa aaaagataac ctattttcaa agaatgtact
    14701 agagttggaa aaagaaatta aacttgcaag tatgtctgac ataccattat ggatgaattt
    14761 agtgagatta gtcatagatg gatttccaaa tttgcaagag gaagaatata tatatcaatt
    14821 agaacagtat attttagaag aaagagcttt aatattaaaa cttaataatg tcgctattgc
    14881 aaatatggtg tttaatagag aaactagaag tattgatttt tttggaatac atccacagta
    14941 tcgtaattca gacattgcac aagtgttttt gaagaaagtt attgaagatt tcttgataga
    15001 tacagatatc tcaattacta cctttcgaga gggtgacaaa gctgatacag gacatagaga
    15061 tatgataaag aagttggggt ttgcagaagc agagttgctt gtggaatttg gatatcctac
    15121 tcaaaaattt attcttcctt gtgtagaaga aagagataat agacaaaatt aattgtagat
    15181 taaatgcaaa gtttaccaca tattttttgt ttgaattatt ttatatataa gagtatttct
    15241 gcttttaatg tattttgctt gtaagtgtac ttatggtaat aaatatagaa agatttagtt
    15301 ttttaaaaaa agaactttaa agctaaaaag ctaacattta agttaaatag caagaatgat
    15361 taaaaactcc aaaacacaat taagtataat gattcagtgt aagtacttaa ttaacaaagg
    15421 aggatttaaa tgtctgaaaa taaacaagta gtaaattgcg atattattgc atccaattct
    15481 aaacataata tgtgtgaaca tgatgtagag ttattaactt tttttccaga tgagattgca
    15541 catctaatag agataaataa caagctagac aatgctttta agaaagctga aaatcttgtt
    15601 gacaagcttg ataaggatta tatggacgct aaaatgtata tggtaaaaaa tcgtggtgaa
    15661 atagacccac atgaaatgtt tcaaaatgaa caaggactaa aacaaataga taactatggg
    15721 gcttttatgg taaaagttcg agataagatt aataaaatca aagattctcc atactttgct
    15781 agaatagatt ttaggctgaa agatatggat gatgaatcta agtattatat tggtagattt
    15841 gcatttgatt atgaagatga gcttataata cttgactggc gttctccaat tgccagtatg
    15901 ttttatgatt atgaaatcgg aaaagctgga tatgatgctc caataggatg ggtggatgga
    15961 gaaataacaa gaaaacgaca atttaaaatt aaaaatggaa aattggaata tgcactagaa
    16021 agttccatta atatacaaga tgatattctt caaaaagaac ttagtcacac ttcagatgaa
    16081 aaaatgaagt caatcatttc aacaatacaa aaggaacaaa atcaaattat tagaaatgat
    16141 aaagctgata cactgattat tcaaggggta gctggttctg ggaaaacatc tatagcatta
    16201 cacagaattg cttttcttct ttatcgattt aaagataaaa tatcagcaaa taatgtaatt
    16261 atcctatcac ctaataaagt ctttggagac tatatttcca atgtccttcc tgagcttgga
    16321 gaagaaccat tatgtgaatt aagttttgaa aatatagcag aggtacaatt agatagagtt
    16381 attaattttg aaagtgagaa agacccatta gaaataaatg atgctaaatg gtctgaaaga
    16441 gtgcgtttta aatctacact tgattttgta aaacttattg atgattatat aaaacaaatg
    16501 ccaaataaga tttttattcc aaaagactac acttttggaa gttttacagc taaaagtgac
    16561 tggatacaga gtagatttga ggcatataac agatatccag ttaagaagag attagaaaag
    16621 gtagctgaag acattcatta taaatttgaa tcagataata ttatggaaga agatttacca
    16681 aaggtaaaaa gtatacttaa aagtttgaat ggaatgttga caataaaaaa tactcttaca
    16741 ctatataagg atttctttaa acaaatgaat atatcaaaca tgtttgtgat ggcagaaaag
    16801 aaaacacttg aatggtcaga tgtataccca tttatatata ttcatgctgc atatgaaggt
    16861 atacaagaag ataagattat aaggcatatt gttattgatg aaatgcagga ctatacacct
    16921 attcaatatg ctgtaataaa cttacttttt aagtgtaaaa agactattct tggagatttt
    16981 ggacaattgg ttaatcctaa tcatactcat acattagatg atatgaaaca gctatataat
    17041 gatggggaac tagttatgtt gaacaagagt taccgttcta cttttgagat tattaatttc
    17101 gccaaaaaga ttcaagatat atcttcatta gagcctattg aacgacatgg tgaagaaccg
    17161 gctttattaa aatgtaataa cgagcaagat gaaataaata agataaaaat agaaattgaa
    17221 gaatttaaaa agagtgataa tgcgactctt ggtattatat taaagacaga taatgacgct
    17281 aaagctgtat atgatgcgtt aagagaagaa tatagtgttc acttaatatc ttctgaaagt
    17341 tctagcttta caaaaggagt atccataact tctattaaaa tgtcaaaagg tttagagttt
    17401 gatgaagtta ttgtaccttc agtaaataat aaaacatatt atagtgatta tgaccgcagt
    17461 ttgttatata ttgcttgtac acgagctatg cacaaactta agttaacata tgttggtaca
    17521 ttaactcaac ttataagcat gtagattata tcttatatgc atttattatc gtaaatataa
    17581 attgggacaa agattcatca ataaatcaac aaaaaataat acttataaat ttataaaaaa
    17641 tgaaagctat aaattacatt tgaaatttta taaaattttg tgatttatag cttttataat
    17701 attaatttct tttaaaataa agttaaactt taaatttatg aaataaaaca aagtagtaat
    17761 atatatgtaa tagttcttaa ttttttaata ttagtattat attatctata tatttattga
    17821 ttttctaagg aaatctgatg ttatagtgca acctctttca aacatttcta gtggtgtacc
    17881 ttcaaatacg acctgaccac cgtttttgcc accatcagga ccaatatcaa ttacccagtc
    17941 tgcttgtttc aatacatcca aattatgttc aattataatt acagtattac ctttatcaac
    18001 tatatgctca aaaatcaaca taagcttttc aatatcagaa ccatgaagcc ctgtagttgg
    18061 ctcatctaaa atatatatag tacctttttt gttgaggttt ttagctaact taattcgttg
    18121 acgttctcct ccagatagtg tgctaagtgg ttgacctaag gtcatataag gtaaaccaac
    18181 atctttcatt gtttgtaaga ttttacatag cttcttttct gtaaaaaatt caagagattc
    18241 atctactgtc atagatagta catcaactat acatttacct ttatataatt ttgacaaaac
    18301 ttcttcacta tatctagtac ctttgcaagc ttcacatgta ttaataatag ggtccatgaa
    18361 tacaagctct gtaacaagta ctcctttacc atcacaaaca ggacaagctc cctttgaatt
    18421 aaagctgaat aaaccttctt caacattatt ttctttagca aatacttttc ttatctcatc
    18481 aaagaaacca agaaatgtag caggcataga acgatttgta gctgtaactg ggctttggtc
    18541 gattttaata acatcttttt cgtatttttg tgcaaaaaca cgagagatta aagtactctt
    18601 gccagaacct gctactccag taacgacagt cataattcca actggtaaat ctacatcaat
    18661 atgttttaga ttatttatgc aagcatctct aattggaagg gtaccttttg gatgacgaga
    18721 gtcagttttt ataggtaatg atagattcat tgcatttgaa gtaagagtgt ttgctaaaag
    18781 taatttatca taagagcctt ggaatacaac atgtccaccc tgagtaccag cacttgggcc
    18841 aatatctata acttcatctg ctatggagat aacatcttta tcatgctcta ctacaaggac
    18901 agtatttcct ctatctctaa gtttttttaa tagctgattc atacgatgta catctcgtgg
    18961 atgcatacca gcactaggtt catcgaaaat ataagtcata cctgtgagac tgctccccat
    19021 atagcgtaca agttttaatc tttgagcttc tcctccagat agagatgatg attctctatt
    19081 tagatgtagg taagggagac caatatcaat cattcttttt aaaccatcag taagagtctt
    19141 tattacagtt tcagttcttt tatcttttat ttgtaacaat aaatcataaa ggtcagtaag
    19201 ttccatattg cacatatctg caatcgaata tcctaaaata ttacagttaa gagtttcttt
    19261 atttaatctt tttccatgac atacaggaca ttccttatct gttataagtc tttctgattt
    19321 ttcttttgtg tgtttgctca tattgctaat atctcgattt agatatgttt gattgtattt
    19381 gttaaaaata ccctctattt ttggattttc ttgttttcca tctttatttt ttgaaccata
    19441 aagcaataaa ttatattctt caaaagaata atcttttata ggtttatcta aatcaaaaag
    19501 tccagactct gcatactgct tccagtacca tgaattaggt ttaaataaag agtcatttat
    19561 acatcctgta ttccatgaat tttcaggatt tataatagat tcaatattga ttactttaac
    19621 tctaccaatc ccagaacaat ttttacacat accatttggg tcattaaaag aaaaatatga
    19681 agttgttcct atgtagggtt ctccaattct agaaaataat aatcttaatg atgaatacaa
    19741 atcacttata gtgccaactg tagaacgtgc attgccacca agaggagttt ggtctacgat
    19801 tacagaagca gttaaattat caatcagctc cacctgaggt tttctatatt taggtagcct
    19861 atttctgata aatgaagaat atgtttcatt catctgcctt tgtgattctg ctgcaattgt
    19921 atcaaatacg atactggatt ttccagaacc agaaactcca gtaaaaacaa caattttatt
    19981 tttaggaatt ttaaatgata tattttttag gttattttgt gtaagtcctt ttattataat
    20041 atttgacatg aatgttaaat cctttctgag tacattttag taaagcaatt atattatgta
    20101 accatattat aataaaatta aaatctactt tcttgatata ttctgctatg ttatagaagg
    20161 tatttctaaa tttaaccaat cccatacaat ctgaataaat gatagttgat aatagataaa
    20221 aaagtatata atagatactc gtaatttgat aagtacaaag ataattatac aattaaaacg
    20281 acttgacatt taaaacttac tattgtaata tttaaatatt acaatagtaa gttttaaata
    20341 gagaataaat aattacctca caaactgaaa ggaggacata atgatactaa tagttatatt
    20401 gagtatattc atcatagcct caatcaacat aattatatta ggtaaagaaa atataagaga
    20461 tatagttatc cctttaactc tatttattgt aagtgatttt ttatttttgt gtggaaatat
    20521 aatatgtagg ggaggagtaa tgctactatt tataggttta tttgcaatac cattgatgct
    20581 aattgcattt atatggattt gtgtacatat gattaagctt tactataaaa aagatttaaa
    20641 taaaa
//
LOCUS       DAEPOZ010000045        20121 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.045, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000045 DAEPOZ010000000
VERSION     DAEPOZ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20121
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.045"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            110..739
                     /locus_tag="KR440_001300"
     CDS             110..739
                     /locus_tag="KR440_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698357.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIIAECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINNFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            740..1522
                     /locus_tag="KR440_001301"
     CDS             740..1522
                     /locus_tag="KR440_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0698358.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAINTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            1515..2393
                     /locus_tag="KR440_001302"
     CDS             1515..2393
                     /locus_tag="KR440_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0698359.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNEEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRIIGKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIKIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKIKEFNIEDGYNRDLTSEYYI
                     KLKKDILMTMKSNKDI"
     gene            2426..3385
                     /locus_tag="KR440_001303"
     CDS             2426..3385
                     /locus_tag="KR440_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH0698360.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDVALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            3699..4391
                     /locus_tag="KR440_001304"
     CDS             3699..4391
                     /locus_tag="KR440_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0698361.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            4401..5405
                     /locus_tag="KR440_001305"
     CDS             4401..5405
                     /locus_tag="KR440_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0698362.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGIHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKDVNSLIKM
                     "
     gene            5653..6504
                     /locus_tag="KR440_001306"
     CDS             5653..6504
                     /locus_tag="KR440_001306"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBH0698363.1"
                     /translation="MSLDVIFILKSVIIAIVEGFTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            6608..7354
                     /locus_tag="KR440_001307"
     CDS             6608..7354
                     /locus_tag="KR440_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0698364.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALFRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            7354..9270
                     /locus_tag="KR440_001308"
     CDS             7354..9270
                     /locus_tag="KR440_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0698365.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTYFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRTNGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKPISFEDNECISLLSSSLPGNNNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLIYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIRLNLVNF"
     gene            9549..10466
                     /locus_tag="KR440_001309"
     CDS             9549..10466
                     /locus_tag="KR440_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBH0698366.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSILDMINSQLEYNNDIYKNEFNNIVEKIKAVLN
                     TGEKSDTYMDYFTVQAQRNCDYKSEKNKCNEIIRAYTTKHKFWIEYYSLNSGNSERIV
                     HPYGLFNYKSDTYMVAFCEKRFKFIDFKLCRIKDYKVLEEKYNVDKSFSWDEYSKNSI
                     GIYKGEEINVVIKISHPFSIIIKEKVWVNNQQIIEYDDKSIMFKAKMRGYEEIKSWIL
                     SMGAYVEVVEPDRLRNDILSEIEKMKKIY"
     gene            10544..11281
                     /gene="cas6"
                     /locus_tag="KR440_001310"
     CDS             10544..11281
                     /gene="cas6"
                     /locus_tag="KR440_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBH0698367.1"
                     /translation="MKMKIEFSTGCIPISYNSLFMSIIKEAIRKSNEDYYKNMYYYKE
                     KNNKKTKNFTFSVYIKKYSIEGDNFIIEDKVILNISTPDLELGFHIYNGLMTSKKCLY
                     KAYELTRIRIDLSREKKVTEERVLFNALSPICVKSKEGKFLEITDDRYIEELNYITNE
                     VVKNYRGNGLKRKLEFENIGLKKIVVKESLREFKKITGKEYQYINGYKGKFILKGDID
                     DLNLIYNLGIGFRRAQGFGDVDILEWR"
     gene            11287..13164
                     /gene="cas8a1"
                     /locus_tag="KR440_001311"
     CDS             11287..13164
                     /gene="cas8a1"
                     /locus_tag="KR440_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HBH0698368.1"
                     /translation="MKLRVYRGDWFFNMGIVGFLNIIKKADKQAEIFIMEDYIEFDSL
                     FLENFHEYYFNYFMDEYDVSKRIKKNIDYSINFIKSKPDRIKDGIKKIKDSVKQQNDK
                     IKKFDEEKFNLIKEKLDSMSKIKSYDEFDSLEALVDETIDIFKIKSINDRLTANLYKY
                     VVQDNYFGQVSFFNVAKAKLDLDGLKQIMFNDYLRQIIYFGELADLLEENDYDKLKNY
                     LNDRLNSIAKDINEKKVSKSSINTIEKIMKEINKNFIKKNKSIEDIKEYMDSLEVCEM
                     CGLYKGILDEYSESNFAPLGVSTNNARNMFWNQAYVPSICDICKLILFCTPAGATYTR
                     KNYLINEENEFYLFVNMDTSINELFERNNSLKAQKSEYSDSKDENPFNQLIKSIVEEN
                     TLKSEWQLRNILFVEFKASIDSKKCKINYFNIPTYLAKFFTDKYANKKIQSIYDYKLK
                     SNVLDLLLKNRDLKHLTNNILRNKVKNDMESNNKSNISGIDCFRVVQLRALINSYKKG
                     VYKMDSKNLEKNDEKLRIIYYLGCDIHDYFVNKNSKNKIDGVSYKLLNSVKVGNKSDF
                     MDTIIRVFMSAEKQIPAFILDIEIEKDLDFESIGHAFISGLISGKYEGKDKLPNEKEE
                     K"
     gene            13166..14080
                     /gene="cas7i"
                     /locus_tag="KR440_001312"
     CDS             13166..14080
                     /gene="cas7i"
                     /locus_tag="KR440_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HBH0698369.1"
                     /translation="MKRGLTFTVIAQAQSLNYGEGIGNISELKKLSRDDGNIYTFASR
                     QCLRYDIVRLAAELFNWNLQVVDKEKGTIQFKDNISIRESEEMDLFGYMKTNKKDEKD
                     KKGGSLTREATVRLSNAISLEPYRSDMDFLNNKGLADRIGEHPNLANIEQHLSYYTYT
                     VTIDLSKIGKDGDIELDNKEKCRRVVEFLEIIKVLNRNIRGRQENLSPLFVVGGVYEI
                     ANPFFLGRIKLKGDKNGFKINKQAIEEVIQGTFLGKDLKEFTYVGMVDGVFINKEEFK
                     GLFEDNFLSVDKFFGQLVKEVKEYYGVN"
     gene            14082..14783
                     /gene="cas5b"
                     /locus_tag="KR440_001313"
     CDS             14082..14783
                     /gene="cas5b"
                     /locus_tag="KR440_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HBH0698370.1"
                     /translation="MKVLKLDLFQETACYKKPFAMKITETYPLPPYSTINGLIHKILN
                     ATEYIPFNISVQGTYESIFNNYQTTYFYKKDSITSMPMNSHMLLNVNLIIHIGGDFDL
                     LEEIYDSLLNYDEHLSLGRKEDLVRINDIRFVEVNKFEVDEDIQLDDYENYKLSECNI
                     RMPIYIPKTSLQDTDIDGISYRLNNYYDNNAVEDKKRVWNKVDSYYVEDGNTISIGDI
                     LLDNEGDLLYFNNIN"
     gene            14794..17115
                     /locus_tag="KR440_001314"
     CDS             14794..17115
                     /locus_tag="KR440_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBH0698371.1"
                     /translation="MLYAKSNPVETLREHTDELLKQMNVLRESYGKNINSLDFLEEEI
                     FWQLLDFVIEFHDIGKAFSPFQELIKSRMDTKINEPKIVTHLENNVGHNYLSPAFIDY
                     SYIDRKKNKELRAVLNQVIVYHHERDIFIDKDFKNLIQKILDEDLINKVYELQREFKV
                     RYPIKTEKLSKVYLQSVEKRIDKNHKYYNLYIMLKGILHRLDHSASAHEVVECNNVIN
                     IGEQTENYLMKEFGSLREAQSFAKSNRNKNIILIASTGMGKTETALIWIDKDKAFFTL
                     PLRVSINALFDRAKNIIGVGEANDTFLGLLHSTAIDYLEESNQENSSEIVDLAKLLSC
                     KLTFSTIDQIFKFPFLYRGYEKVYSTLAYSKVVIDEIQAYSPEIAAVLVKGIEMIHKI
                     GGRFMIMTATMPTIYIDELKKRGVMNSNLADLTCNTEKIRHCVSIVENSIDENLGKII
                     QSGMNKKVLVIVNTVKSAVEKYELIEEIVKPKGIDVNLNLLHSMYIQEDRAKLEKYIK
                     EFADSDSNGIWITTQLVEASLDIDFDELHTENSTLDSLFQRFGRCYRSREYEENSPNI
                     FIYTEKATGIGSIYDKDIVEKGLELLKTFINGKECVKMKEKYKVEMVKILYSKESLEG
                     TAFEKRFTSALNILDTITPYSLGSKDAQNILRNIEGYTVIPEDIYNKIEETLIKDYEE
                     LGQELINAYSNDDRQLINKIKSKRKKARREIIKKTVNLPIYKAKQNVIDIQVKGLEDL
                     KILLYKYDIHENKHTKQIFGKGVLISNEIDQFI"
     gene            17130..17681
                     /gene="cas4"
                     /locus_tag="KR440_001315"
     CDS             17130..17681
                     /gene="cas4"
                     /locus_tag="KR440_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HBH0698372.1"
                     /translation="MWLKFTKEGDFVPIDLEVLKVQGVKINYYYVCKRKLWLFSKGIT
                     MEQNSSRVLSGKIVHEDSYKRMKNKEMLIDDILKLDIIKDEYVKEVKISSKMKEADEM
                     QLYYYLYYLKNSMGIEKKGTINYVKEKKQEELILTEEKEKIIENTLININEINMQLSP
                     PKIEKKHYCTKCAYYEFCFVKEE"
     gene            17684..18664
                     /gene="cas1b"
                     /locus_tag="KR440_001316"
     CDS             17684..18664
                     /gene="cas1b"
                     /locus_tag="KR440_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HBH0698373.1"
                     /translation="MYKDYYIISNGNIKRKENTIYYYNLEGEKKILPVEQVDDIHIYG
                     EVDLNTKLINYISKYGVMLHFYNYYGYYSGTYYPRKKNVSGFSLVCQSACYLDKNERL
                     YLAKSFVESASYHILRNMRYYKVDESLIDTIKDELNDLNDVKEISELMGKEGRIRKTY
                     YKSFNQILKDDFEFYKREKRPPKDPVNTLMSFGNSIMYTNVLSEIYKTQLDPTISFLH
                     EPSTKRFSLSLDIAEIFKPIIVDTIIFSLINNKRINKNDFEYQDKICYLSEKGKKKFL
                     QEFENKMQTTIKHRNLNRKVSYRMFIRLECYKLIKHFINDERYKVLKVWW"
     gene            18667..18933
                     /gene="cas2"
                     /locus_tag="KR440_001317"
     CDS             18667..18933
                     /gene="cas2"
                     /locus_tag="KR440_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HBH0698374.1"
                     /translation="MFVIVTYDIVEARSLNRIRRILRKYLTWTQNSVFEGNITDGKLH
                     KCISEIENIIDNSEDSIYVYEIKNPNSIKKKCYGIDKYSDEMFI"
     repeat_region   19117..20074
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=19117..19145
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
BASE COUNT         8192 a         1782 c         3486 g         6661 t
ORIGIN      
        1 tagataaaaa ttgttttatt aaggaaaaat taatatattg acaaaattta agtgctcatt
       61 taaactatta attgataata atattcaata ttaaataata ggaggcgtta tgaataaata
      121 tcttctaata aatccagtgg cagaaagaat gtatggagaa aagtttcacc ttattaaagc
      181 aaatttgata aaaaaggggt acataatagc agaatgtgaa ccacagttag aatatgtaaa
      241 aaagcagtac aaagagtata caaaaaagac tgaaaataca atgttggatt gtagatgtcc
      301 agaaagtata agtcttttaa aaagaaataa tttgataaat aattttgaag tacctatgat
      361 agaaccaata ttagtaagga cttgtagggt tttatacgat aaatatataa aaagtaaaga
      421 tgatatgttg gtagtaacct gtccatgtac acaattaaga gactttgcaa gtgaaaagtt
      481 taaggataag ggtaatgcta tgttttatac atggaaagag tttgctgaac aagaaggtgt
      541 aaaatcactt ggaaaaatag atgaatcacc aattccatta ggatatttca aagatacttt
      601 tgaaaaagtt cttgaagtat caagtgaaga cgaaatatta agtgaaatac aaaaaataag
      661 aaatggcttt gaagataagt atgacatagt agaaatgttt tattgtaaag atggttgtaa
      721 taatggagat ggtttgtaaa tggtcaataa atttaataat aagtttttaa ggctagtaaa
      781 gagtgttctg atatttttta taattctttt attatggaaa attacaaatt atcttggtat
      841 ttggagtgat tatatattac catcacccga aaaggtatat agtacatttt taaatatgat
      901 aagtgatggt tcaatattta ttaatgtata tgcaagtatg aaaagagttt tgataggttt
      961 tgcaataaat actgctatag gtgtgccttt agggatattc tttggaattt atagtggagt
     1021 atacgaatac tttaaatctc taataaattt tttaaggaat acacctcccc ttgctttaat
     1081 accaatgctt atattatggt ttggtatagg agaggaatca aaaattataa ttatagtttt
     1141 agcatccttt ttcccaatct ttacaagcac attgaaaggg attaaaaatt gtgactcaaa
     1201 actgattgaa gtgggtagag tatttgagtt ttcaaagctt cagataatat ttaaaataat
     1261 aataccaaat gccattcttg atattgctgt tgggttaaag ttagctcttg gatatagctt
     1321 tagagcaatt attggggcag aacttgtagc agcttcatct ggtcttggat acttgatatc
     1381 tgatggaaaa gaaatgtcta gaacagatgt tgttattgta ggtataatcg taattggact
     1441 tttaggaata attacagatt acatattttc gattattgtg aagaaggtaa gtaagggaaa
     1501 gatggtggaa gcatatgaat aatttagtat gtgactctgg atatataatt agaaatttat
     1561 ataaaagtta taaagttgac aatgaagaac atgtagttct tagtgatatc tctttaaaca
     1621 tagatagaaa ccatataaca gtaattttag gtgaaagtgg ttgtggaaag actaccttgc
     1681 ttagagcaat tgcatcactt gaaggtataa atagtggtga gattaggttt attaatgata
     1741 ataaagagta tagacctaat gttggttttg tttttcagga gagcagatta atgccttggt
     1801 taaacgtaag tgaaaatata gcttttcata atcaaagaaa aaatactatt ttaaacaagg
     1861 tgcttagaaa atttaaacac aataaaagaa ttattggtaa aattataaat gataatttta
     1921 gaagcttaga aacaaatgat aatttgaaca aatcaaataa atataatagg aaaagtaata
     1981 ttgatgtaga aaaatatctt aagatgatga acttgcagaa atttaaaaac tcttatccaa
     2041 atgaattgtc aggaggtatg gcacagagag taagcatagc tagagctctt tctttcaatc
     2101 cagatatgtt gcttatggac gagccttttt cggcattaga ttattttaca aggattgata
     2161 tgcaaaatga agttataaaa atacataagt caactaataa aggcgtaata tttgtaactc
     2221 atgatattga tgaagcgtta aagataggca aaaaaatagt tgttttcaca gacgaaagaa
     2281 aaataaaaga atttaatata gaagatggtt ataatagaga tttaacttca gagtactata
     2341 ttaaattaaa aaaagatatt ttaatgacaa tgaaatcaaa taaagacatt taaagaaatt
     2401 aataatattt taaggagtag agtaaatgaa attaaaaaag ctattgatag gtataacgat
     2461 attaacactt gcaacagcat ctttagctgg atgtgctaaa aaaaataatg gagaaaaact
     2521 ttcagaaata aatttaactt atgtaaaatc accattaaat gttccttcta taattcagaa
     2581 acaggatgat ttatttggaa aggaatttaa aaaggataat ataaaagtta actttcacga
     2641 aattactaca ggtccagagc aaacacaggc tttagctgct ggagaaatag acttcttaca
     2701 tgcactaggt ggtacgtcag ctttaatagc tgcatctaat ggagtagagt taaagatatt
     2761 aaacacttat agcaggtcac caaaaggatt tatgattttg actaataata attcaataaa
     2821 atcagcagca gatttagtag gtaagaaggt ggcaggtcca aaagggacta tattacatca
     2881 ggtattgatt tcagcacttg ataaagaagg attaagtatg gatgatgtgg aatttgttaa
     2941 tatgggtatt ccagaagcat cagcagctct ttctgatggt agcgtagatg ttgcattgat
     3001 tgctggtcca gcagctctta aagctatgaa gtcaggtagt aaacttgtag caaatggtga
     3061 aggtttagta gatggtataa tagtaactgc tgttagcaca gactttgcag agaaacaccc
     3121 agaaatagtt gaaagattta tgaaggttga aaaggaaact ttagaatatg taaataataa
     3181 ttttgatgaa gctatggaaa aagtagctaa agaagttgat ttaagtcttg aagaaactaa
     3241 agaattatat gcttggtatg attttagttt agatataaca gataaagata tatcttcgct
     3301 tgaagataca caagacttct taataaaaaa taagttacaa gagaaaaaag tgaatataaa
     3361 agaattaatc tataatgcta agtagaaact atgctaaaat aatactaagt aagaaatata
     3421 ataaaagttt aagttacctt attaaggtaa ctttttctat tttgaattag taatttcaat
     3481 ttattttaga atgattagtt gataataatg attaattaat cataaaaaga tactttttta
     3541 tagagtttta ttaagatgtc taataatatg gttatactta tgaagtcagt aattgtgaag
     3601 ttaataatat gattaaattt atatattgta aaaagattat aatatattaa aattggactg
     3661 ttatatgatg agtaaatagg tatattagga ggaattatat gagttataaa gtatatattg
     3721 tggaagatga tatatcaata agtacattac ttaaagatta tattgagaaa tatggatttg
     3781 aagtgattgt tgaagagaac tttgatgata taatgattac atttgaaagt tttaagccac
     3841 acttagttct attagatgta aatttaccta aatttgatgg tttctactgg tgtagatgta
     3901 ttagacaaca gtcgaattta cctataattt ttatatcagc tagagatggc aatgtagacc
     3961 aaattagagc tttagaaagt ggagcagatg attatataac aaaacctttt tactatgatg
     4021 tagtaatggc aaaaataaag agtcatataa gaagggtata tggtgaatat tcacctaaaa
     4081 tagacgaaag aatagtggag ctagatggat taaagtttta tccagaaagg ttagaactag
     4141 aattaaatgg tgaaaatcta attataacta aaaaagaagg tatactgatt gaatgtctta
     4201 tcaagagata cccaaaagta gtaagtagag attatctttt agaaaagata tgggatgata
     4261 ttgagtttgt agaagaaaat actttaaatg taaatgttag caggataagg aaaagatttg
     4321 aggaattagg tatagataat gctgttcaga ctgtccgagg gttaggatat aaattaaata
     4381 agaattggta aggagaaagt atgaagttat ttttaaaaga ttataaaggt tatattttta
     4441 tatattatat aggcatagtt atgacaatta tttactgtaa tcttatgaag tttattagtt
     4501 ttggagagag tttatatatt cttttattta gttcttttat attgttatgt tttttaatat
     4561 ataagtatta taaaaataga gaagtatata atctgtttga aaaaggaata cattctttgg
     4621 aggagtcatt tttgtattta ggaagctcat tcttggggag aaatatatca gttattttaa
     4681 aacaacaaca taaattgtat caatcaatga tacaagagca taaaaagata catagtgaac
     4741 atttaacttt catcaaccaa tggattcatc agatgaaaac accaatatca gttataagcc
     4801 ttcagcttca agattatgag ggagaagaag taactgaaaa tataagaaaa gaaatagata
     4861 gattaaataa agggttagat atggctatgc attttgcaag ggcagataat tttcagaaag
     4921 actttatagt tgaaaaagcc aaattaaatg aaattgtatc tagtattgta aatgaagaaa
     4981 aaaggttatt tataaaaagt ggaatgattc ctaagattca agtagacaaa tttacttatg
     5041 tttatacaga tgtaaaatgg atgaaattta taataggtca attaattaca aatggagtga
     5101 aatattctaa agattatagt aactacctaa ctataaaatc tgaagaaaat aataaatatg
     5161 taattttaag tataatagat gaaggaattg gaattgtttc taaagattta aaaagggtat
     5221 ttgacccatt ttttacagga gaaaatggtc gtaagtttgg agaatctaca ggtatgggtc
     5281 tttatctagt aaaaaaggta tgtgatgatt taggtcataa agtaagtata aaatcagaag
     5341 taggaaaagg tacagaagtt tcaatctact ttaataaaga tgtaaatagt ttgataaaga
     5401 tgtaaattat ctgttacaat agggttttta gttaattgta cttttttatt atacaattta
     5461 ttaaatgaaa tattcatagt taaataatca aaatttaaga gagtttattt gcaaacctta
     5521 catagatgta aggtttgtgt aatgtttgca gatagttaaa ttttctaatc taaattacaa
     5581 ttaatataag aaataacatg agagaaataa cataaaaaaa gaaattttgt aaaaaaggaa
     5641 ggatgtttat tgatgagttt agatgttatt tttatattga aatcagtcat aatagctata
     5701 gtagaggggt ttacagaatt tattccagtt tcatctacag gtcatatgat tttggttggt
     5761 aatttaatag attttaaggg gcaatttgct gaaatgtttg aggttgtaat acaacttggg
     5821 gctatattag cagtagtagt actttattgg aaaaagataa aagatagcgt tatagaattt
     5881 tttaaattta tatttactgg cggaaaagaa ggaaagattg gttttagatt tggaatgaat
     5941 gtaattatag gttgtatacc atttgcgatt ataggagttc tattttatga taatataaaa
     6001 tcattgttta atttacaatc tgtaattata ggatttatag tgggtgggat tttattatta
     6061 gtagtagaaa ctttgtttag aaaaaagaat cattcaacag ataatataga taaaattact
     6121 ccaatacaag ctttaaaggt tgggacatta caagttttgt ctgcatggcc aggtatgtca
     6181 aggagtgcat ctactattat gggtggatgg atagcaggac ttaattctcc tacagcagct
     6241 gagttttctt tctttttagc agtaccagct atggtggctt cttcaggaaa agatttattt
     6301 gaatttgatt attcaataat gacaccaaca ttgtggattg ctttggtagt tgggtttata
     6361 gtagctttta ttgtatcaat aatagttatg gaaaaattcg tgaatttttt aaagaaaaaa
     6421 ccaatgagag tgtttgctgt atacagaata atcatgggtg tagtacttgc agtattagct
     6481 tttactaaca taattagtgt atagaaaaaa tagatttatt atctataaaa attaaactaa
     6541 gataaattat aattaacaat tatatatatg ttatttcaaa aattaaatta ggaaggatag
     6601 ttaaataatg aaaattttaa ctgtaaataa tttaagtaaa gtatatggta aaaaaataat
     6661 tttcaacgca ttaaatgaca taaattttag tatagaagat ggagaatttg taggaattat
     6721 gggtccatct ggtagtggaa aaactactct tttaaatatg atttctacta ttgacaagcc
     6781 aactacagga actatggaat taaagggaaa gaatccttta ttgttaagag gtgaggaact
     6841 tgcattattt agaagaagag agttaggttt tgtatttcag gattttaatt tattggatac
     6901 attaactata ggagaaaata tagtattgcc attgacatta gataaggtca gtgtaaaaga
     6961 gcaagatgag agattgaatg aagtatctac aatattaggt ataaaagatt tattaggaaa
     7021 aagaactttt gaagtatcag gtggtcaagc tcagaggact gctattgcta gagcattgat
     7081 taacaatcca tcaatacttt tagcagatga accaacaggt aatttagatt caaagtcatc
     7141 aaaggttgta atggagctat ttcaaaagat aaataaagaa aataaagtta ctactatgat
     7201 ggtaactcat gacccattag cagcgagcta ctgtagtaga atcttattca taaaagatgg
     7261 gtctatatat aatgaaatat ataagggtag tagtagagag cagttttatc aagagataat
     7321 ggatgtactt acattattgg gaggcgataa ctaatgaata taaaacagtt tgctattaac
     7381 aatatttcaa gaaatattaa agcgtattta ggatacttag caagtattgt tatatcatct
     7441 tcactgctat tttcttttat tatgtttaca agtcatccag atttagatgt atctcaattt
     7501 cctgattatt taaaagaagg tctcaagatg agtaagataa ttgcatatct attcttattc
     7561 ttctttgtgt tttattcagt tagtgtgttt cttaaaagta gatataaaga atttggaata
     7621 ctttatatta caggtatatc aaaacgccaa gtaatgaaat taatctttat agaaaatatg
     7681 ataataaata tagtatcttc tgtaattgga ataataatag gcttaatctt tgcaaagata
     7741 tttttagtgg ttatgtctac ttttttagaa ttatcgccat taaaattcta tataccattt
     7801 aatgcgatat tgtctacttt aatctatttt atgattttag ctgtattaac atcaatattt
     7861 acttatttta tagttaaaga aaatcaggtg ttgaagttac ttaaaggtac aaaaactcca
     7921 aaaccagagc ctaaaacatc actaatgctt gctatattaa gtatttgttt gtttataata
     7981 gcgtattata atgctattac ctcaaccgac caatatacaa catatctgcg cttagttcca
     8041 gttacatccc ttactatagt agcaacttac ttgttctttt ctcaatttag tgtatttttt
     8101 atgaagaagc taaaactaaa caagagattt tatagaaaaa atacaaatat gctttggatt
     8161 tcgaacttga tttataaggt aaaggataat gctagaatat ttttcttgat aactataact
     8221 tctgcagtag catttactgc tataggtaca gtttattcct tttggaaaga tgtagagcgt
     8281 caaattaatt taatatatcc taacacaata tattattcaa caatgacatt gcataatgac
     8341 cctaaaaagc ctgatagcaa agaaaaagac aaggaaagaa tgatgtttat agaaaataat
     8401 ctaaaaaaag agaatgtaga ctatacaagg actaatggtg aatttaaaac tgtatttccc
     8461 aagaaaagtg attttaatgt cagaattatg aaagaatcta agtacttaga aattacagaa
     8521 actatagggg ttaagcctat aagttttgaa gataatgaat gcatatcatt attatcatca
     8581 agtttaccag gaaataataa cgtcaaagaa aatattattg tagggaatag aggtttaaag
     8641 gtggcaaaac aagtggaaga atgtgttatg ccagcatatt acaaaaatat gtatgtggta
     8701 aaggataatt tttatgatag tatagaatca aaatttatag tggatagatt tactgcattt
     8761 gaagtagagg attcaagtaa aactatagag atatgcagag aatttgaaaa taaatttaat
     8821 aatgagtcag ggatgcaacc atatttgttt ttctctaaag aattaatgat aggaactggg
     8881 aaattaatat attctacatt tatattttta gcaatattta taggtttaat tttctttgtt
     8941 actacaagta gttttttata taataaattt tatatggatt gccaagttga taagaaaaag
     9001 tatgagcaat taaataaatt ggggatgaca tataatgaga ttaaaaaagc ttcaacaatt
     9061 gagattggaa tagtattttt gtttccttat gtagtggctg ttatacattc aatatttgcc
     9121 cttactgcac tgaaaaattc ttttgatata gaagtagcat catcagctgt tttggtaatg
     9181 ggaagcttgt tcttggttca aatagtttat tttttaattg taagaaataa ttatttaaaa
     9241 gagataaggt taaatttagt caatttttag ttactattat taaatgataa taaaaagggt
     9301 ttaaaattaa ttaatattac tggaattata gatgtttatt ttttcagtaa tattttttat
     9361 tgtattaact attaaaattt tgtacaaata taaaatataa tatggtagat gtcaaaatta
     9421 taaaaaaatt ctacttatat aaaatataaa gaggttatat gtacatatgt gtagaaataa
     9481 ataataaaaa tatcaattaa tgttattagg gggggttact cattagtaaa ttagctaata
     9541 cacttagaat gatgatatta attcaaagca gaggcaaaat gaaatgcaaa gaattatcag
     9601 aagagttgga agtaagtgaa aggcaaataa aaagttacaa gatgtattta gagcaagctg
     9661 gtatatttat aaattcaaca cctgggattt atggtggtta tgaaatagat aaatgtaata
     9721 gcatatcatt aattaaatta ttagatagtg aagtttcaat tctagatatg ataaattcac
     9781 agttagagta taataatgat atttacaaaa atgaatttaa taatattgta gaaaaaataa
     9841 aagcagtatt aaatacagga gaaaaaagcg atacatatat ggattatttt acagttcaag
     9901 cacaacgtaa ttgtgattat aagtctgaaa aaaataagtg taatgaaata attagagcat
     9961 ataccacaaa acacaaattt tggatagaat attattcatt aaattcagga aatagtgaaa
    10021 gaattgttca tccatatggt ttatttaatt ataaatcaga tacttatatg gtagcttttt
    10081 gtgaaaaaag atttaagttt atagacttta aattatgtag aattaaagat tataaggttt
    10141 tagaagaaaa atataatgta gataaaagtt ttagttggga tgaatatagt aaaaatagta
    10201 taggaattta taaaggagaa gaaattaatg ttgttataaa aattagtcat ccattttcaa
    10261 taattataaa ggaaaaagtt tgggttaata atcaacaaat catagaatat gatgataaat
    10321 ctataatgtt taaagctaaa atgagaggtt atgaggagat aaaaagttgg attttaagta
    10381 tgggagctta tgttgaggtt gtagaaccag atagacttag aaatgatata ttgtcagaga
    10441 ttgaaaaaat gaaaaaaatt tactgatttt ttcaaataat ttatacttgg accaatatac
    10501 ttcacggtct agtgttaata tattaattga gagaggtgat attatgaaaa tgaaaattga
    10561 atttagtaca ggttgtatac caatatctta taattcgctc tttatgagta ttataaaaga
    10621 agcaataaga aaatctaatg aagattacta taaaaacatg tactattata aagaaaagaa
    10681 taataaaaaa actaaaaatt ttacattttc ggtttatata aaaaagtata gtattgaagg
    10741 tgacaatttt attatagaag ataaagtaat attaaatata agtactccag atttagaatt
    10801 gggttttcat atatacaatg ggttaatgac ttctaaaaaa tgtttatata aagcctatga
    10861 acttacaaga attagaatcg atttatcaag agaaaagaag gtaacagaag aaagggtact
    10921 atttaatgca ttgtctccaa tctgtgtaaa gtctaaagaa ggaaaattct tggagataac
    10981 tgatgataga tacatagaag aactaaacta tataactaat gaagtggtta aaaattatag
    11041 gggaaatgga ttaaaaagaa agttagaatt tgaaaatata ggtctcaaaa agatagtagt
    11101 taaagagagc ttaagagaat ttaaaaaaat aacaggtaaa gagtatcaat atattaatgg
    11161 ttacaaaggt aaatttatat tgaaaggtga tattgatgat ttaaatctta tctacaatct
    11221 aggaattggt tttagacgtg ctcagggatt tggagatgta gatattttag aatggaggtg
    11281 agataaatga agttaagagt ttatagagga gattggtttt ttaatatggg tatagttgga
    11341 tttttaaata taataaagaa agcagataaa caagctgaaa tatttattat ggaagattat
    11401 atcgaatttg atagcttatt tcttgaaaac tttcatgagt attattttaa ttactttatg
    11461 gatgaatatg atgtatcaaa aagaattaaa aaaaatattg attacagtat aaattttatt
    11521 aaatctaagc ctgatagaat caaagatgga attaagaaaa ttaaagatag tgtgaaacaa
    11581 caaaatgata agattaaaaa atttgatgaa gaaaaattta acctgataaa agagaagcta
    11641 gatagcatgt ctaagataaa aagttatgat gaatttgaca gtttagaagc tttagtagat
    11701 gagactatag atatatttaa aataaaatca ataaatgaca ggcttacagc aaatctttat
    11761 aaatatgtag ttcaagataa ttattttgga caagttagtt tttttaatgt tgctaaggcg
    11821 aaattagatt tagatggatt aaaacagata atgtttaatg attatttaag acaaattata
    11881 tattttggag agttagcaga tttattagaa gagaatgact atgataagtt gaaaaactat
    11941 ttaaatgata gattaaatag tatagctaag gatataaatg aaaaaaaagt tagtaagtct
    12001 agtataaata ctatagaaaa aattatgaag gaaataaata aaaactttat aaagaaaaat
    12061 aaaagcatag aagatataaa agagtatatg gatagtttag aagtatgtga aatgtgtggt
    12121 ttatataagg gaatattaga tgaatactca gaaagtaatt ttgctccact tggagtaagc
    12181 accaataatg ctaggaatat gttttggaat caggcgtatg taccatcaat atgtgatatt
    12241 tgtaagctaa tattgttctg tactccagca ggggctactt atacaagaaa gaattattta
    12301 attaatgaag aaaatgagtt ttatttattt gttaacatgg atacttctat aaatgagctt
    12361 tttgaaagaa ataatagttt aaaagctcaa aaaagtgaat attcagattc caaagatgaa
    12421 aatcctttta atcagcttat aaaaagtata gtagaagaaa atacattaaa aagtgaatgg
    12481 caacttcgta atatactttt tgtggaattt aaagcaagta tagattctaa aaaatgtaaa
    12541 ataaattatt ttaatattcc aacttattta gcaaaatttt ttacagacaa gtatgcaaat
    12601 aaaaaaatac aaagtatcta tgattacaag cttaaatcaa atgtactaga tttactcttg
    12661 aaaaataggg acttgaagca tctaactaat aacattttaa gaaataaagt taaaaatgat
    12721 atggaaagca acaacaaatc aaatatatca ggaatagatt gttttagagt agtccaatta
    12781 agagcattaa taaatagcta caaaaagggg gtatataaaa tggatagtaa aaatttggag
    12841 aagaatgatg aaaagctgag aataatttat tatttaggtt gtgatataca tgactatttt
    12901 gttaataaaa attctaaaaa taaaatagat ggagtaagct ataaactttt aaattcagtt
    12961 aaagtcggaa ataaaagtga ttttatggat acaattataa gagtttttat gtcagctgaa
    13021 aaacagattc cagcatttat tttagatata gaaattgaaa aagatttaga ctttgaatct
    13081 ataggtcatg cttttatatc tggattaatt tctggaaaat atgaaggaaa agacaaacta
    13141 ccaaacgaaa aggaggaaaa ataagatgaa aagagggtta acatttacag taatagcaca
    13201 ggctcaaagt ttaaactatg gggaagggat aggtaatata tcagaattaa aaaaattaag
    13261 tagagatgat ggaaatatat atacatttgc atctcgccag tgtttaagat atgacatagt
    13321 acgattagca gcagaattgt ttaattggaa tttacaggtt gtcgataaag aaaaaggtac
    13381 tattcaattt aaagacaata taagtataag agaatcagaa gaaatggatt tatttggata
    13441 tatgaaaaca aataaaaaag atgagaaaga taaaaaagga ggttctttga ctagagaagc
    13501 tactgtaaga ttaagtaatg caatttccct tgagccatat agaagtgaca tggacttttt
    13561 aaataataaa ggattagcag atagaatagg agaacatcct aatcttgcta acatagagca
    13621 acatttaagt tattatacat atacagtgac tatagattta tctaaaatag gtaaggatgg
    13681 agatatagaa cttgataata aagagaaatg tagaagagta gtagaatttt tagaaataat
    13741 aaaagtattg aatagaaata ttagaggtag acaagaaaat ttatctcctc tatttgtagt
    13801 aggaggagta tatgaaatag caaatccatt tttcttgggt agaattaaat taaaaggaga
    13861 taaaaatgga tttaaaataa ataagcaagc tatagaagaa gtaattcaag gtactttttt
    13921 aggaaaagat ttaaaagaat ttacctatgt tggaatggta gatggggtat ttataaataa
    13981 agaagaattt aagggattgt ttgaagataa ttttttaagt gtagataaat tttttggaca
    14041 actagttaaa gaagtaaagg aatattatgg ggtgaattaa tatgaaggtt ttgaaattag
    14101 atttatttca agaaactgca tgctataaaa aaccatttgc tatgaagatt acagagacat
    14161 atccactgcc tccatattct acaataaatg gattaattca caaaatatta aatgctacag
    14221 aatatatacc atttaatata agtgtacagg gaacatatga aagtatattt aataattacc
    14281 agacaacata tttttataaa aaagatagta ttacatctat gccaatgaat agtcatatgc
    14341 tattgaatgt aaatttgata attcatatag gtggggattt tgatttattg gaagaaatat
    14401 atgatagttt attaaattat gacgagcact tatcattagg tagaaaagaa gatttagtaa
    14461 gaattaatga tattagattt gtagaagtca ataaatttga agtagatgag gatatacaat
    14521 tagatgatta tgaaaattat aagttatcag aatgtaatat aagaatgcct atatatatac
    14581 caaaaacaag tttacaagat acagatatag atggtataag ttacagactc aataattact
    14641 atgataataa tgctgtggaa gataaaaaaa gggtttggaa taaggtagat agttattatg
    14701 ttgaggatgg aaatactata tcaattggag atatattatt agataatgaa ggagatttat
    14761 tatattttaa taatataaat taggagggag agtatgttat atgctaaatc taatccagtg
    14821 gaaacactta gagaacatac tgatgaactt ttaaaacaaa tgaatgtatt aagagaaagt
    14881 tatggaaaaa atataaatag cttagatttt ctagaggaag agatattttg gcaattactt
    14941 gactttgtta tagaatttca tgatattgga aaagcatttt caccgtttca agagttaata
    15001 aaaagcagaa tggatactaa aataaatgaa cctaaaatag taactcattt agaaaataat
    15061 gtaggccata attatctttc tcctgcattt attgattata gttatataga caggaaaaaa
    15121 aataaagaac ttagagcagt attaaatcaa gttattgtat atcatcatga aagagatatc
    15181 tttatagata aagattttaa aaatttaata caaaaaatat tagatgaaga tttaataaat
    15241 aaagtttatg aactacagcg tgaatttaaa gttagatatc caataaaaac agaaaaattg
    15301 agtaaagtat atctacaaag tgtagaaaaa agaatagata aaaatcacaa gtactataat
    15361 ttatatatta tgttaaaagg aatactacat agactagacc atagtgcttc tgctcatgaa
    15421 gttgtggaat gcaataatgt tataaatata ggagaacaga cagaaaatta tttaatgaaa
    15481 gagtttggta gtttgagaga ggctcaatca tttgctaaat ctaatagaaa taagaatata
    15541 atactgattg catctacagg aatgggaaaa acagaaactg ctttgatttg gatagataag
    15601 gataaagcat tttttacact accattaaga gtaagtataa atgcattatt tgatagggct
    15661 aaaaatataa ttggagtagg agaagcaaat gatacattct taggtctgtt acattcaaca
    15721 gcaatagatt atttagaaga aagtaatcag gaaaattcaa gtgagattgt agatttagca
    15781 aagttattat cttgtaaact gacattttcc acaatagacc aaatatttaa atttcctttt
    15841 ttgtatagag gctatgaaaa agtgtactca acattagcat attcaaaagt agtaatagat
    15901 gagatacaag catattcacc agaaatagca gctgtattgg taaaaggtat agaaatgata
    15961 cataagatag gtggcagatt tatgataatg acagctacta tgccaaccat atatatagat
    16021 gaattaaaaa aaagaggtgt aatgaatagt aacttagctg acttgacatg taacacagaa
    16081 aaaataagac attgtgtgtc tattgtagaa aattcaatag atgaaaactt aggtaaaata
    16141 atacaaagtg gaatgaataa aaaagttctt gtaatagtaa atacagttaa aagtgctgta
    16201 gaaaaatatg agttaataga agaaatagtt aaaccaaaag gaatagatgt caacttaaat
    16261 cttttacatt ctatgtatat acaagaagat agagcaaaat tagaaaaata tataaaagaa
    16321 tttgcagata gtgatagtaa tggtatttgg attactacac aattagtgga agcatccctt
    16381 gatattgatt ttgatgagct tcatacagaa aattcaacac ttgacagtct atttcaacga
    16441 tttggtagat gttatagaag tcgtgagtat gaagaaaata gcccaaatat atttatatat
    16501 acagaaaaag ctacaggaat aggtagtatt tatgataagg atattgtaga aaaaggactt
    16561 gagctactga aaacttttat aaatggaaaa gaatgtgtga agatgaaaga aaaatataag
    16621 gtagaaatgg ttaagatact atactctaaa gaaagtctag aaggtacagc ttttgaaaag
    16681 agatttacca gtgctttaaa tattttagat actataacgc catatagttt aggttctaaa
    16741 gatgcacaaa atatacttag aaatattgaa ggatatacag taattccaga ggatatatat
    16801 aataaaattg aagaaacttt aatcaaagat tatgaagagt tgggacaaga gcttataaat
    16861 gcatatagta atgatgatag acaattgata aataaaataa agtctaagag aaaaaaagca
    16921 agaagagaga taattaaaaa aactgtaaat ttaccaatat ataaagctaa acaaaatgta
    16981 attgacatac aggtgaaagg tcttgaagat ttaaaaatac ttttatataa atatgatata
    17041 cacgaaaata agcatacaaa acagatattt ggtaaaggtg tgcttataag taatgaaata
    17101 gaccaattta tttgattttt atattaacaa tgtggctaaa atttactaaa gaaggtgatt
    17161 ttgtgccaat agatttagaa gtattaaaag ttcaaggcgt aaaaataaat tattattatg
    17221 tatgtaaaag aaaactatgg ctattttcta aaggaataac tatggaacaa aatagtagta
    17281 gagtattgtc tggaaagata gtacatgaag attcatataa aagaatgaaa aataaagaaa
    17341 tgttgattga tgatatcttg aagctagata taataaagga cgagtatgta aaagaagtaa
    17401 aaataagttc aaagatgaaa gaagcagatg aaatgcagtt atactattac ttatactatt
    17461 taaagaatag tatgggtata gagaaaaaag gaactataaa ttatgttaag gaaaagaaac
    17521 aagaagaatt gatattgaca gaagaaaaag aaaaaattat tgaaaacaca cttataaata
    17581 taaatgaaat aaatatgcag ttatctcctc ccaaaattga gaaaaaacat tattgtacaa
    17641 aatgtgctta ttatgaattt tgttttgtga aggaggagta acaatgtata aagactatta
    17701 tattataagt aatggaaata taaaaaggaa agaaaatacg atttactatt acaatttaga
    17761 aggtgagaaa aaaatattac cagtagaaca agttgatgat attcatatat atggagaagt
    17821 agatttaaac acaaaactta taaattacat aagtaaatat ggggtgatgc ttcatttcta
    17881 caattactat ggatattatt caggtacata ttatccaagg aagaaaaatg tatcaggatt
    17941 cagtttagta tgtcaatcag cctgctattt agataaaaat gaaagattat acttagcaaa
    18001 atcctttgtg gaaagtgcat cataccatat tttaagaaat atgagatatt acaaagttga
    18061 tgaaagttta attgatacta taaaggatga actaaatgat ttgaatgatg tcaaagaaat
    18121 atcagaactt atgggtaaag aaggcagaat aagaaaaaca tactataaat catttaatca
    18181 aatattaaaa gatgattttg aattttataa aagagaaaaa agacctccca aagacccagt
    18241 aaatactctt atgtcttttg gaaatagcat aatgtatacg aatgttttaa gtgagatata
    18301 taagacacaa cttgacccta caataagctt tttacatgaa ccatcaacaa agagattttc
    18361 tttaagttta gatattgcag aaatttttaa gccaattata gttgacacaa taatttttag
    18421 cctgattaat aataaaagaa ttaataagaa tgattttgaa tatcaagaca aaatttgtta
    18481 tttaagtgaa aaaggaaaga agaaattttt gcaagaattt gaaaataaga tgcagacaac
    18541 tataaaacat agaaatctaa atagaaaagt ttcatataga atgtttataa ggttagagtg
    18601 ttataaatta attaaacatt ttataaatga tgaaaggtac aaagttttaa aggtgtggtg
    18661 gtaagtatgt ttgttattgt tacttatgat attgttgaag caaggtcgtt aaatagaatt
    18721 agaaggatac ttagaaaata tttgacttgg acgcaaaatt ctgtttttga aggcaatatt
    18781 actgatggaa agttacataa atgtatttct gaaatagaaa atattattga taatagcgag
    18841 gattcaatct atgtttatga gataaaaaat cctaattcaa ttaaaaagaa atgttatggg
    18901 attgataagt attctgatga aatgtttata taggtttgca gtgagcgata tttatgctaa
    18961 aataggtgtt aacagttgga atataaggga ttgaaggtgt atgataactg ttatcaattg
    19021 cactactgct cgctcactgc aaattttgat gtttttattg aattataatt gcttgattga
    19081 agtattttca atgtattcaa ttatacctat tttggggttt tatattaact aagtggtatg
    19141 taaataagca actatagtag aagcaaaagc gacaacaact aaaggtttta tattaactaa
    19201 gtggtatgta aatttggtta acttaagtgc tatgaaagga attaatgaag ttttatatta
    19261 actaagtggt atgtaaattt aaaggtgcaa gtcttaatac ttgttacatg agttgtttta
    19321 tattaactaa gtggtatgta aataacaaag aaccatttga atcacgaaaa ctaaaaaaca
    19381 gttttatatt aactaagtgg tatgtaaatt tattaacctt atcagaaacc tgaccatccc
    19441 aattatagtt ttatattaac taagtggtat gtaaattcca tttgtctaat gcttctaact
    19501 ctttttttat acgttttata ttaactaagt ggtatgtaaa ttccaaaatg ggcttggagt
    19561 taagagttgt ataagaaacg ttttatatta actaagtggt atgtaaatta attacacact
    19621 cccttttaac gaacatattt tagattgttt tatattaact aagtggtatg taaatagact
    19681 cgacatagaa tcggttacta aatccgaggg tttcaggttt tatattaact aagtggtatg
    19741 taaatactaa acctttttgt ttacttttat ttctcatttt acgttttata ttaactaagt
    19801 ggtatgtaaa tccggatttt tctgcttcga ctgttgttat tgagacttgt tttatattaa
    19861 ctaagtggta tgtaaatagt aattccatgc aatcttttat attgcttttt aacctgtttt
    19921 atattaacta agtggtatgt aaatagatat acaaggtgaa agtgttgaaa tttcaatctt
    19981 gttttatatt aactaagtgg tatgtaaata ttcttagatg ttataatttg tgttgcattt
    20041 aaatcgtttt atattaacta agtggtatgt aaatacaata ttattgttca tttcatttaa
    20101 taattctagt agttttatat t
//
LOCUS       DAEPOZ010000046        19581 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.046, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000046 DAEPOZ010000000
VERSION     DAEPOZ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19581
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.046"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..1360
                     /locus_tag="KR440_001318"
     CDS             <1..1360
                     /locus_tag="KR440_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0698375.1"
                     /translation="FEGLFMFYIQNYYYDSVKQLLESEIKYADEYNAITMETTSFEKK
                     VKNIFDKQPLTKNSEFGISIIDKDKNIILDQYGFKSKEKANYEDVNNALKDIKTKNLT
                     PYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYTVSLDAIDNAILKQATWLILAGIFIL
                     IIAILISLKFAETLIKPLRELKKFANELAVGNYNIKLEKMKIVDDEIGDLAQTFEHMA
                     HEIDKSEKLKEEFISSVSHELRTPLTSIKGWSETLGYESITREELDLGLGIIQDETER
                     LIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIVGVVNQLKVKAAEKDISLLFEFENEF
                     IENIQGDKNRLRQVLINLIQNSFKFTSQGGYIKVVASQDEEITTISVEDNGSGIEKQN
                     LNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHGGRMKIESEKNVGTKITFNIKNKFAK
                     QA"
     gene            1382..2119
                     /gene="truA"
                     /locus_tag="KR440_001319"
     CDS             1382..2119
                     /gene="truA"
                     /locus_tag="KR440_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBH0698376.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    2230..2437
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2690..3889
                     /locus_tag="KR440_001320"
     CDS             2690..3889
                     /locus_tag="KR440_001320"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBH0698377.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            4136..5182
                     /locus_tag="KR440_001321"
     CDS             4136..5182
                     /locus_tag="KR440_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HBH0698378.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGECLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DGGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDERIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPSKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNDSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTEQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            5290..6453
                     /locus_tag="KR440_001322"
     CDS             5290..6453
                     /locus_tag="KR440_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0698379.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFNVLEEFKREHRNKIIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAQAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     SKNPSSTDLQEIAKEITDISKKDYEIFSMYTGDDNWYYMIQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(6542..6922)
                     /locus_tag="KR440_001323"
     CDS             complement(6542..6922)
                     /locus_tag="KR440_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HBH0698380.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(6925..7584)
                     /locus_tag="KR440_001324"
     CDS             complement(6925..7584)
                     /locus_tag="KR440_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBH0698381.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
     gene            complement(7690..8664)
                     /locus_tag="KR440_001325"
     CDS             complement(7690..8664)
                     /locus_tag="KR440_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBH0698382.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDIIIYLKQILTILNISKKEIYFKKRSVLVNNSKIINFKNLSSSFKL
                     IKLNEFEEYPDFILKLGDKKSFVESVIKINKSNRKSIKNLILKLSKKP"
     gene            complement(8704..9051)
                     /locus_tag="KR440_001326"
     CDS             complement(8704..9051)
                     /locus_tag="KR440_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698383.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(9269..10225)
                     /locus_tag="KR440_001327"
     CDS             complement(9269..10225)
                     /locus_tag="KR440_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBH0698384.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTIYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIQAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
     gene            10666..11034
                     /locus_tag="KR440_001328"
     CDS             10666..11034
                     /locus_tag="KR440_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HBH0698385.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNKVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
     gene            11459..11704
                     /locus_tag="KR440_001329"
     CDS             11459..11704
                     /locus_tag="KR440_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698386.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVVNKVKTG"
     gene            11803..12066
                     /locus_tag="KR440_001330"
     CDS             11803..12066
                     /locus_tag="KR440_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HBH0698387.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(12351..13298)
                     /locus_tag="KR440_001331"
     CDS             complement(12351..13298)
                     /locus_tag="KR440_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HBH0698388.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALATEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(13301..14932)
                     /locus_tag="KR440_001332"
     CDS             complement(13301..14932)
                     /locus_tag="KR440_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBH0698389.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCRVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            15062..15979
                     /locus_tag="KR440_001333"
     CDS             15062..15979
                     /locus_tag="KR440_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0698390.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            complement(16036..16617)
                     /locus_tag="KR440_001334"
     CDS             complement(16036..16617)
                     /locus_tag="KR440_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBH0698391.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(16657..17223)
                     /locus_tag="KR440_001335"
     CDS             complement(16657..17223)
                     /locus_tag="KR440_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBH0698392.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            complement(17678..18538)
                     /locus_tag="KR440_001336"
     CDS             complement(17678..18538)
                     /locus_tag="KR440_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBH0698393.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
     gene            18687..19577
                     /locus_tag="KR440_001337"
     CDS             18687..19577
                     /locus_tag="KR440_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0698394.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLSPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAV"
BASE COUNT         7333 a         2518 c         2858 g         6872 t
ORIGIN      
        1 gtttgaagga ttgttcatgt tttacataca aaactactat tatgattctg tcaaacaact
       61 acttgaatct gaaataaaat atgctgatga atataatgct attacaatgg aaactacaag
      121 ttttgagaaa aaagtaaaaa atatatttga taaacaacca ttaactaaaa attcagaatt
      181 tggaatttca ataatagata aagataaaaa tattatatta gaccaatatg gttttaaaag
      241 taaagaaaaa gcaaactatg aagatgttaa caatgcatta aaagatataa aaactaaaaa
      301 tttaacacct tatacttata gaataccaga tacaggggag catgttatga gtatctcctt
      361 accacttaag gtaaataata taatagaagg tgttgttaga tatacagtat cattagatgc
      421 tattgataat gcaatattaa agcaagctac atggcttata ttagcaggaa tatttatact
      481 tattatagct atactaataa gtcttaaatt tgctgagact cttataaaac cattacgaga
      541 gctaaagaaa tttgcaaatg aattggctgt aggaaattat aatataaaat tagaaaagat
      601 gaagattgta gatgatgaga ttggtgattt agcacagaca tttgaacaca tggcacatga
      661 gattgacaaa agtgaaaaat taaaagagga atttatatca tctgtatctc atgaactaag
      721 gactcctctt acatctataa aggggtggag tgaaacctta ggttatgaaa gtataacaag
      781 agaagaacta gacttaggtt taggcattat acaagatgaa acagaaagac ttataaaact
      841 agttgaagaa ttattagact tttcaaggct ttcttcagac aggataaaac ttcatgttga
      901 tatagttgat gttgaaggtt taattgtagg tgttgtaaat caacttaaag ttaaagcagc
      961 tgaaaaagat atatcacttt tatttgaatt tgaaaatgaa tttatagaga acattcaagg
     1021 agataaaaat agattaagac aggttctcat aaatttaata caaaattcat ttaaatttac
     1081 gagtcaaggt ggttacataa aagttgtagc ttctcaagat gaagagataa caaccatatc
     1141 agtagaggat aatggttcag gtatagaaaa gcaaaattta aataaagtgt tagataaatt
     1201 tttccaagaa gattataata aggctggtag tggattagga ttagcaatta gtaatgaaat
     1261 tgtaaaactt catggtggaa gaatgaaaat agaaagtgaa aagaatgttg gaacaaaaat
     1321 aacttttaat ataaaaaata aatttgctaa acaagcataa attcttagaa agaggattaa
     1381 gatgagaaac ataaaaatga tagtagcata cgatggttca agatataaag gatatcaaaa
     1441 attaggtgac aataatatga ctattcaaga aaaacttgaa aatgttttaa gtaaaatgac
     1501 taatgaaaca gtggagataa tagggtctgg aagaactgat atgggagctc atgcaagagg
     1561 acaagtggta aattttagaa ctaattgtat ggattctttg gataaaattc aaaaatattt
     1621 atatgaatat ttaccagaag atatagttgt aaaaactgtt gaagaagttg atgaaagatt
     1681 tcattcaaga tacaatgtaa aatcaaagac ttatatgtat aaaattgaca ataataagta
     1741 tcacaatcct tttataagaa aatatgctac tcatgtaagt aaaaaattag atttagatag
     1801 aatgagaaaa gcaagtgagt atttagtagg agaacatgat tttactagtt ttgcttcttc
     1861 taaatctaag aaaaaatcaa atgtaagaga aatttattct ataaatataa aagaagatga
     1921 taatgttata gaaatatatg tagaaggaaa tggatttttg tacaacatgg taaggataat
     1981 agtaggtgct ttgatagatg ttggtctaaa aagaagagct ccacaagata ttaagtatat
     2041 gcttgaatca aaagatagat gtcaatcatc agatactgca ccagctaaag gactatgtct
     2101 ttggaaagta acatattagt caattatata aaactctctt taaaataaaa ataaaataaa
     2161 ttatatagtt gacataaatg gaaaaataaa ctattatata ttttataaga gagatataaa
     2221 actttatatt taaatcctgt gagaaagaga gtaacttcta tgtgttttat agagagctga
     2281 ggatggtgga agctcagcaa ataagtatta gtgaaaagaa ctttggagga tactaattga
     2341 atttagtatg ttagtaggta atctcgcctt aagagaaaga gtggataagt ttatcttatc
     2401 aattagagtg gtaacgcgga tatatttcgt ctcttggttt ttccaagagg cgttttttaa
     2461 tatatttaat tattagtgtt tatctggtat gttgaacatt tactaattga ttagctaagt
     2521 ttatcaataa gagtggtacc gcggatattt cgcctcttag tattttaaaa tactaagggg
     2581 tttttttagt attaaaatga ttttatgtga gtctaaattc tcagagtcat tcaatattaa
     2641 gaatttctat aaaataaaaa ttataattta agattgggag gaattaaaaa tgaaggaaaa
     2701 agtagtatta gcatattcag gaggacttga cacatcaatc ataatacctt ggttaaagga
     2761 aaattatgaa gatatagatg ttatagctgt ttgtggaaat gtaggtcagg aagataaaat
     2821 ggaagatgta tatgaaaaag ctttacaaag tggagcatca aaggcatatg tggatgatat
     2881 aagtgaagaa tttgtaactg aaactatatt taaagccgta aaagcagagg ctaaatatga
     2941 aggaaaatac ttattaggaa cttctttagc aagacctata atagctaaaa aattagttga
     3001 agttgctcat aaagaaggtg caaaatatat atgccatgga tgtacaggaa aaggtaatga
     3061 ccaagttaga tttgaagcta caattgcagc tcttgacccg actataaaag ttattgcacc
     3121 ttggagaatt tgggatataa aatcaagaga agatgctata gactatgctg aaaaacataa
     3181 tataaaggta acagcaacta aagctaaaat atattcagta gatgctaacc tttggcatgt
     3241 ttcaacagaa ggtggagata tagagcacct agaaaatgaa cataaaaaag atgtttataa
     3301 acaatgtgta gacccagaag atgcatgtga tgtagctgaa tatgtagaag tttactttga
     3361 aaaaggtgta cctaaaaaag taaatggcga agaattatct ccagttgcac tgattcataa
     3421 gttaaatgag ttgggttgta aacatggaat tggtgttata gatatagttg agaatagact
     3481 tgttggtatg aaatcaagag gtatatatga gactccagga ggaacaatcc tttatgaagc
     3541 acataatatt ttagaaagtg ctactttaga caaagataca ttacatttca aacaaatggt
     3601 atcttataaa tacggagaac ttatttacaa tggactatgg tattgtaaat taagagaatc
     3661 tatagatgca tttatggaac aaactcaaga taatgttaca ggaacagtaa aagttaaact
     3721 ttacaaagga aatataaaac ctgctggaat atttactgaa aatgctcttt atgatgaagg
     3781 aatatcttct tttggaaata gtgaacttta tgaccacaaa gatgcagaag gatttattaa
     3841 tttattcact ttaccattaa aaatcagagc tatgaaagct ggaaaataat aaaataaatt
     3901 atttaagagt gtataaaaac tatttttagg tttttatatg ctcttttttt atttgaattt
     3961 attaaattat acagtaaata gtataaaaaa atatattata gttccaaatg acttaataat
     4021 gtgaaaatat aagacctaaa atataaattt tataaataca atagttattg actctctcat
     4081 tgtaagaggg tttatactga atctaatgaa aatttatagt gaggagatag attatatgag
     4141 aaaaataatt gaaattaaac atcattcaca tgattatgag tgtatgtgga atggtataga
     4201 agatttatat ataaataaaa ccggtgaatg ccttccaaac gattttttct ttttactgtc
     4261 aggttttggt tcattttgtt atatgaaaac aaataaagct gatttgaaaa gaatggtagc
     4321 tttgggagat ggaaggacaa aacagatgta taaattttta gcaccaattg ttggatttga
     4381 atacaaacat catgagttta aaaagtatga gcaagcatta aagaaagcaa aatctgaaat
     4441 tgatggtgga tatccagtta ttttaggagc tttagatatg tattatctac catattataa
     4501 gaagctatat cataaagaac acattccttt tcattatata ttaatggtag gatatgatga
     4561 tgaaagaata tatctgtatg attgtggtag aacagaatta ttacatcttt cttatgataa
     4621 attacgtgaa agcatgaatt gcaattcccc tggattaagc aaagaaaata ctatttgcac
     4681 aataagaatg aatacttcaa agaataaata taacattgct agagaagcta ttgctataaa
     4741 gcgtgatatg tttttaaatc catctaaagg atttttggga tataaaggtt ttgaaaagtt
     4801 tattaaagag ttgccacagt ggaacactga attggataaa gatgattatg ataagatttt
     4861 gaccaatatg gttatgtttt ttggaacagt accaacaatt ccaaatgcaa tcaaaggaat
     4921 taaagaacca gatactgttc aatttaaagg tggatttgat aaaatgagta gaatgctcaa
     4981 cagtattggg gaggaataca aaaacgactc ttttatacaa gtagctgata tttttgataa
     5041 aactgctatt ataattgaga aaatatccaa tattataata gattatttaa ctcaaacatg
     5101 tgatgataca gaacaacttc cgttattgtt ctctgaggtc ttagagcata tgaaaacagg
     5161 atatttaatc ttggatgtat aaaaaatata ttgttgaccc taacattacg taatagtgta
     5221 atatgaaaat atagcttgaa tatattagta ggtattgatg gctgtaaaat taaaatttga
     5281 ggtgtggata tgagaacagt gaaacaagtt tctgatttaa ctggaataag tgtgagggca
     5341 ttacattatt atgatgagat aggtctttta aaaccaaata aaattacaga tgcaggatat
     5401 agactttatg atgatgaatc tattaaaact cttcaacaaa ttttattttt taaggagatt
     5461 gatattcctt taagagaggt taaggaaata atgtcaagcc aatattttga taaggttgaa
     5521 gctctaaaaa atcaaaaaaa attacttatt ttaaaacgaa agagactgga tgaattgata
     5581 gagcttataa atcaaactct aagaggagaa ggaaatattg attttaaaga gtttgatatg
     5641 agcgagtatt ttaatgtttt agaagaattt aaaagagaac ataggaataa gataattaaa
     5701 atttatggta gtgtagaaaa atacaatgaa tatatagaaa gagttaagtc taatgaagag
     5761 aaaattgcta aaatggctat aaaacaatat ggaactattg agaagtttgc ccaagctata
     5821 aaaacaaatt ttagcagtga tatattgaat ttaggagaaa aatttgatag atataaaaat
     5881 gattgtttaa aagaaaaaca tccaaaatta aaagaattat atagaaagct tgtagaagat
     5941 ttaagtaaga atccttcttc aacagacctt caagaaatag caaaagagat aacagatata
     6001 tctaaaaaag attatgaaat tttctctatg tatactggtg atgacaactg gtattatatg
     6061 attcaaaatt atctagtaaa tcctatgtgg atagaagaag tagataaaaa gtatggaagt
     6121 ggagcaggca agtttattgg acaagtacta aagacttatc taagagatag aaaaccaaag
     6181 ataaatacat tgtatgaaaa acttgtagaa gatttgagta gagattgttc ttcaagggaa
     6241 gttcaaagca ttgttgaaga aatagacaat gaaatgaaaa gaagtaatga attttataaa
     6301 atagacaatg gtgaaagata ctttgattat atgtcagagc tttatttaca agattctaac
     6361 tatataaaag taactgataa gaattatggt gatggtgcat ctaagtttat tggagaagct
     6421 tttaagattt attttgataa taataattgt tgaaaatttg gaagtaaaaa atatgtaagt
     6481 tttataaaag cacaaaaata aggatgtaga ttagctccat ctatatcctt atttaaagtt
     6541 attaaaatat atagtttaca tcaaacttaa cactttcaat aatatttccc aaatatgttt
     6601 ttatactttc aacttcttct aaagatttta atttattttc agtataaatt gctttgtcca
     6661 gaatattaaa ccttaatcct atttcatcaa agtactcatg atttaaaact ttgtaccaat
     6721 ggtttttttc tttattccaa ctcgtagata ttgaatcagc ttctttttga gctttttccc
     6781 aatcttcatt ttctatagat ttttctaaaa cagatatatt gtctttatat ccttctgtaa
     6841 aatcatatag tttattgtta acgtaaaatc caaataatac aaataagata gtccatagta
     6901 taacaaatgt tgtagatttc aatattaatc acccttttta ttttgaataa atattttgtc
     6961 attttcatct aaaacacaaa taagaacatc tttaaaagtt tctatgtgat tagattttaa
     7021 aatacccatt aaccagttag tatctttttg agctatgtct agatttttat ttattattct
     7081 accatccagt ataagtggga taggtaaatg attaaaagct cttggaggag tactattgta
     7141 gcttgatgct ggtacaacac ttagattacc atcagtctcc aaaatagcat attgtacatc
     7201 ttttaggttg aagtatcctt gaatcctaag ttgttctaat aattcatcta tactaattct
     7261 ttctttttta agctctttat atacaatttt tcctttatct attaatacag aaggtttacc
     7321 tgataaaaat gaacttaatt ttctgctttt taatgataag aatgaaataa gagtctgcat
     7381 gattacaaga ccagatattg ctgcaacacc atttatgatt ggtatattat tattttccat
     7441 tgggacagat gcaacttctg caattaatag tgttataact aaatcaaaac agttcatttc
     7501 agctatttca ccttttccca tgactctaag tgcaattagc actgttatgt ataatataat
     7561 acttcttatt aatactactg tcatatctat caattactcc tttaaaaaat atttacactt
     7621 agttagtata tcctatataa aaaatttaat tcaaagtata aaaaacctta aataattgga
     7681 ttcaactatt taaggttttt ttgataattt taatattaaa ttttttatgg attttctgtt
     7741 acttttattt attttaataa cagattcaac aaaactcttt ttatctccta attttaagat
     7801 aaaatcagga tattcttcaa attcattgag ttttattaat ttgaaagaag aagatagatt
     7861 tttaaagtta atgattttag agttattcac taatacactt ctttttttaa aataaatttc
     7921 ttttttactt atatttagta tagttaatat ttgtttaaga tatattatta tatcttctgg
     7981 agttctatca aatttgagtt caaaattttc atcttcaaag tagggaatat aatataaagg
     8041 tgttttgaga gcaggtaatt tattatataa ttcttcaaga atatcgatat ttttagggga
     8101 gttaaagaaa aagcatttac atacgttcat taaatccaca gcatctgaaa gtgagttatg
     8161 tttaatatct ccttctacag aaaaaatttt tttcatattc agtaaagata tttgcttaga
     8221 taagattcta ttattgtatg taatatgttg agatataaaa ggttgaatgt caatcatcat
     8281 attaaataac tctaagcatt tacctgtata tttatatact ctaaatgaag ttagaaggcc
     8341 agttatatca aaatcacccc aattatatat tgtaacatca tcaaatttac aaaaccattt
     8401 ataaaaatca tccataacat ataaaagatt aggagcatta ttgatatcta gttggttcaa
     8461 tttggttaaa tttttacatt tatttgaaag tattttattg tattttggct taactaaaga
     8521 gtaataatta tctattgtat taaattcgtt atcaactaaa actccaccaa tagaaattaa
     8581 ttccgcacca ttatattctt ttggtttatt tttattatca tctagtatat taaattcaaa
     8641 gtctataaat aacttgtatt tcataaatct aaagcacatc ctcaggtaaa ttatttattg
     8701 agcctaagca tcataagaat caatagatat aatgactcta aacatcaaag gttcattaca
     8761 acttttattt atttcataat ttaattcaaa taaatctttt aagatataat ttataagtat
     8821 tctttcccaa cccattttta aattaacttt agagtctatt aatatatttt ctatcttttt
     8881 aaatatttct atatttgctg gtattatact gagctcaata ttacttttaa acgggttaga
     8941 ctgtaaatct gttataagta tattttttaa ttcctctgta agttcagaaa ttaaactatc
     9001 acctatgtat ttttttatga tttcctctct aatttctttt aaatttagca atattagtac
     9061 ctgctactta catagtagct tttcctttcc ttttaatact attattcatt tttgaatctt
     9121 atttagatat attttaagat taaaagtctt tagctatata attttttacc catttttaac
     9181 ttatattaac atattacagg cttatatctc caccctgctt acattgagga tggagtatat
     9241 tgcctatttt gataaaaatg ataggttgtt atttgcctat tcctttttct gataattttt
     9301 ctaacatatc tttggtcatt ttatctaaat cataactaaa cttaaagttc cattctttta
     9361 ctgcactact actgtctaga gagttaggcc aagaatcagc tatactttgt cttacagggt
     9421 ctacatcata ttctatcaca aaatcaggaa tatatttttt tatagaatca gctatttctt
     9481 caggagaaaa gctcatagca gtaatattaa aagcatttct atggatgagt ttatccgctg
     9541 gagcttggat taaatccact attgattgaa gtgcatcagg catatacatc atatccattt
     9601 ttgtaccttc tgctatgtaa gatttatatc tcttattttt taatgcctca taatatatat
     9661 caactgcata atctgtagtt cctccaccag gaggcgtaac ataagaaata agacctggaa
     9721 atctaacacc tctagtgtct actccaaatt ttgagtgata ataatcacat agtaattctc
     9781 cagaaacttt agtcacacca tatatagtat taggtctttg taatgtatct tgaggagtca
     9841 tattttttgg agaattttca ccaaaagcag caattgaact aggtgtaaaa aatttacaat
     9901 ccaattcttt ggctatttca aggccattta ttaatccatt catatttaag ttccaagctt
     9961 ctaggggttt actttcagca actgcagaaa gtaaagatgc tagatgaatt atagtatcta
    10021 catcatattt ttttgcaatt tcaaaaaaac gattcttatc tgtaacatct aatatctcaa
    10081 atataccaga ttcacatact ggattacctt cttttactct ccttgaacta gcgattacat
    10141 tttgttctcc atactcattt ctaagcttta ttgttaattc tgaacctatt tgtcctaatg
    10201 cacctgttat aagtattttt ttcaatttta caacctccta agggtttaat ttatgtagta
    10261 ctacttattt ttattttagc aaatcttttg tttaaataca aagaattaaa gataataata
    10321 tacgacttaa ttgaatgaag tgcagtaaat tcctaaaaat ttaaagttga gatataatgg
    10381 tctattgtat aacaataaaa gactagatta aattattata tagattgtgt gactaagtca
    10441 cagaaagtat agttaattta atatataata aaagcatatc aagtaaagct ttaaattaaa
    10501 gtaactttat ataaaaagta atataaattg ataaaattat aaatttattt atagtgaata
    10561 taggttggat tttatagata caaaaatagt atatatgata tgtagaccat tagcatgttt
    10621 aaagtagcaa aacatataaa ataaaatttg aaaggaagta taaaaatgac aagaccatca
    10681 atatatcata gccaaggata cacttgtgca gaggcattaa taaagtctta taatgaggaa
    10741 cacaacacag atataccaat ctcaatagga agtggtatgg gggttggaat gaatgttgga
    10801 agtgtttgtg gagctgtaaa tgctgctgtt gttataatag ggtatttaaa tgggcgaaat
    10861 agtaatttag atgaaaatat agcaaggaag tattcaagag agctattgaa taaagtaaga
    10921 aataaataca gctcagaaat atgtgtatct cttaaaaaaa ataaagtaac ttgtgaagaa
    10981 ataataaatt tcacatatga tgctctaaat gatatgctaa aaaatcaaaa ataattctaa
    11041 gaaacaagta ttttacattc gatagaatac ttgttttttt atgatatttt taataggtat
    11101 tagtttatta taatttttaa atcaatttta ttatatttta attgaaataa tctactttga
    11161 aaattactaa ctgacaaaag aatatgatta tagtatagaa aaaatgtaaa ttatttaata
    11221 ttaagtgaga gtatatgctt acctatataa aatcgtataa attaatggct aaatttataa
    11281 attataaaga aatatataag aaaacaaaag aattaattag tataatataa agttaaaaag
    11341 tgaaaattta tataatttat ttataaagta aagaaaaaat agaaatttgt aatgaaataa
    11401 aaagtttatg acataatatg gtataattag taaaaatata gagattggag gaattgcttt
    11461 gtttatagat gaagaattag aagggtatat attaacatgt aaaatatctg aagactttaa
    11521 aaatatacct gaatatagtg atgaagagtt ttatgttaca gtctataaag atgaaagttc
    11581 tgactctggg tactatgctt tattagaaaa taaagaagaa agagttgtat gggatggaga
    11641 agttgttgcc aataatattt ttaataacct ttggattgta gtaaataagg ttaaaactgg
    11701 ataatagtct aattatatag tatttgtaat attttaaatg aatttttaca tatttgagat
    11761 gagtaaataa caaagattat tcgatactgg aggagtaatt caatggaata tagttatagt
    11821 aagatgaact taaaaaaagg ggatattgta gaggtaaatt tagagaaaca agccaatgta
    11881 atattgttag accatataaa ttatgtgaaa tttaaaaatc aaagaaatta tgattactat
    11941 ggtggatttg caaagaaaaa tccatgtaga atgagagttc ctaatactgg aacatggtat
    12001 ctagtagtaa atcaagatgg aaatagtgga atagtaaact tttcaatcaa tacaattcaa
    12061 aattaatttt agaatattgg atattcttac tttttgtact ttttatatag ttaaaataaa
    12121 attaataagc aattattata aaataatgtg ggtgcctcaa aatgaatttt ataattcacg
    12181 agacactctt ttttgtataa actcaaatat actttatcat ttcaacaatg aaaaagaggc
    12241 gtatctgtgt tttgagatag cctcatttat catatatcat atatagaatt ttaattattc
    12301 ttaactaaaa ttttttccat aggttatctg tacaattaaa atatttattg ttagcataca
    12361 gctccacctg taaggtgtaa aagagcctct agtatctctt cttttctttc caaaacttca
    12421 tctgataaaa gcatttctat aaatttatca gcaccaattc tatcgatagt tattccaaaa
    12481 cgttcacctg tgttaccctg ttctctaaat aaaagaattg ctttttcaat taaagtcata
    12541 agttcctctt ttgaaaataa tctatcaatc tgtgttccag gacgaactga ttttccccat
    12601 ttccctccta tatagacttt ataaccagat tctttttctt caatactatc aaaattacaa
    12661 ctttctatac acttaccaca gttattacat agattactgt ctatttcaag tttaccttta
    12721 tctgttaatt tagctgcttt aacaggacag acctctacaa cagcacattt tttacaacca
    12781 acacaaagct ctgaatcata gtcaggtaca cgttgaccaa caattccaag atcgtttagg
    12841 tcaggtttaa tacagttatt agggcatcca ccaacaccaa ttttgaactt atgaggtagt
    12901 tttacatcat accaattttt gtaaaattct tcgtgtattt cagtagcaag tgcctgtgta
    12961 tcagctagac catgaacaca aacagtacct ttacaagcca caacaggacg aacccttgat
    13021 ccagttccac ctgttattag attttcgcta gcaataaatt cacttagagc ttcaatatct
    13081 tcaaatttta caccaggtaa ttctactgtt agtctagtag taaatgctaa ctgaccgttt
    13141 ccgaattttt cagcagcttc tgcaattttt tttgcttgtg atgcatttat tacaccattt
    13201 acagttatta tacgtgctgc aaaatgttct ccatccctgc ttggtagaaa acctctacct
    13261 tttacttcct ttttttgttc tgctgtaaca ttactcatat ttactcctcc attaccttaa
    13321 gatttggata aaatagtatt ccaccttcaa gaactttcgt atttgtatag ccaaagcgtc
    13381 ttaagcgatt ttgcgccata tagctatttt tacctttagc acatataaga agaatttttt
    13441 catccttagc aagaccttca atttctccat taatttcacc aacaggaata taacgtaaac
    13501 taggaataga aggagctttt gaaacatcaa gtttagtcca actgtcaaaa tcctcaattt
    13561 cgtcaagtaa aacactatca agttctccgt taagtttatt ttgtaaaaca tttactgcca
    13621 ctacaaaagg gtgaatagct gttgaaaaag gtggtgcata tgcaaggtcc atactttcta
    13681 atgaataaag gtcagcgcct aaagttattg cagtagcagc gatatctact attttatcaa
    13741 ccgctccagg tcccataact tgaacaccta ataattttct agtatttttt tcagcaacca
    13801 ttcttataat aaaattagat gcacctggat aataatgagc tttatcatcg gtagctatgg
    13861 ttacagagca aacattataa ccttcttttt cagctatttc tttacttaga cctgtttttc
    13921 ctgcattgag ctcaggtaat tttactactg tcgttccaag aacaccattg taagttttag
    13981 attttccaga tatattttgt gcgcaaattc taccctcatg atttgcagat gaccccatag
    14041 gtgaccatgt aggtttattt gttaaagaat tttttacaaa agcacagtct ccaactacgt
    14101 atatatcttt atcatttgta agcatatatt catctgcaat aattgtatta ttaggggcaa
    14161 attctaaacc tgtgtctttt agaaaacctg tgttaggacg gattccaact gacataacca
    14221 ctaaatctgt tttaatagct ctatttttag tacgaagttt ttcaacttta ttttcacctt
    14281 caatacctac aacctggtca cctgtaaaaa tcattatgcc tttatcagcc atatgatttt
    14341 caacgtattc agcaaaatca gtatcaaatc caggcataac atgttcagcc atatcaacaa
    14401 cacttacacg tatacccatt gcagaaagat tttcagcaat ttcaagacca atgaaacctc
    14461 caccaataac aacagctctc ttaataccgt tttcaataga atctcttagt tttatagcat
    14521 catttggagt acgcataaag aatactccag gtaaatctat tccaggtatt ggtggtttta
    14581 ttgggtctgc ccctgtagca attacaagtt tgtcatagct tagattttct ttttctcctg
    14641 ttgaaagcgt tttcaaagtt acttgttttg catttctatc aatagacgta acttcaactc
    14701 tacaacgaac ttctgctcct gttagtttag aaaaactttc aggtgtattt acgattaaag
    14761 atgatttatc ttcaattatc ttaccaacgt aatatggaag accacaacct gcataagaaa
    14821 tatcatcacc tttatttagt attgtaacag agcaattatc acccatttca cgttttattt
    14881 ttgcagcagt ttttgttcca gcagcaactc cacctataat taatactttc acaatatatc
    14941 acactccaat ctactttata actctataat aaaattatac ttcttgacaa tatatttgta
    15001 aaatactaaa atattaataa aatcataagc atttactaat gtttgctaag gttgtgataa
    15061 tatgacaatt cgtagtcttg aaatctttgt taaggttgct gaatgtggta aaatgagtga
    15121 agtagcaaga aatatgtata ttacccagtc atcagttagc caagctattt cagaaataga
    15181 aaaagagtat ggtgttaagt tatttgatag aatttcaaaa aaattatatt taactgaggc
    15241 agggaaaaaa cttcttggat atgggaggca tcttttagct gtaaatgaag aaatgaatga
    15301 ttgtatgaag cattgtgcaa aaaatatccg tataagagtt ggggcaacag taactgttgg
    15361 tacatgtgta ataagcccga taatgttaga attatacaag gtaaatcctc ttatagaacc
    15421 tgaagtattt gtagaagata caagactgat tgcaaaaaaa ttacttaata gtgaattaga
    15481 tattgcgatt gtagaaggaa aaattaagca cccagatatt gttacaaaaa gcattattaa
    15541 tgataatctt gtacttatat gttcacacaa gcatgagttc tacaaaagag actctataaa
    15601 agtttcagaa ctttcaaatc aaccattaat tatgcgtgaa ttaggaagtg gtacaagagc
    15661 acaattagaa aagcaattaa aagaattaaa aatcccaatg aatattagat ggtcatgtta
    15721 taactctgaa gctatattaa gagcagttgt agataatttt ggcattgcag ttatatccga
    15781 attacttatt gaggattatt taaaaaagca tcttttgtgg gcttgtgata ttgaaggtat
    15841 taacttacat agaacattcg atattgcata tcatagaagc aagtttttta cagaaaatat
    15901 ttcagcattt tttgatattt cagtagaata tgggaaaaaa caagcgagaa agaagagtag
    15961 aagtgtaaac agtttataat atatctaaat aaaaataaga gtatatcaat atactcttat
    16021 ttttatttag atatattagc ctatattaac gccaaaattt ttacacaatc ctgcaagtcc
    16081 atcctcaaat ccagaaccta aagcattaaa tttccactct ccattatgtc tatatagttc
    16141 tgccacgact attgctgttt ctatgctaaa atcttcaccc aattcatatt taataagttc
    16201 ctcgttagtt tcttcattat atattcttat atatgaattt tcaacttgtc cgaaattttg
    16261 tcttcttgta attgcttcat ttatagtaac actaaatgat attttagcaa tattttgagg
    16321 tattttagat aagtctacat ttatttgttc atcatcacca tcaccaacac ctgttctatt
    16381 atcacccata tattcaacag ctccagaagc gtgttttaaa ttattataga atataaagtc
    16441 tccatcattc gttacttttc catcagttcc agttaaaaat gcagaggcat ctaaatcgaa
    16501 atcaaatcca ccatcatatt tattaatatc ccatcctagt cctactatta ctttttttag
    16561 accagggtta ctttttgtta aacttacttt ttgtcctttt gctaaagtta tacccattta
    16621 aaccaactcc tcattataaa ttttttattg taattattac ttatatgtat taattaattc
    16681 acgaagctct tcattttgac caattcctaa agcattaaat ttccattctt catttctacg
    16741 ataaatttct cctgccaaaa ctgttacttt accagaaaag tcttcttgaa gattatattt
    16801 aaataattct tgcttggaat ttgaatcaat taatcttata aaagagttgt tcatttgacc
    16861 aaaatcttgg tctctttttt tagctttgta tatagaaact ccaaaaacta ttttatgtac
    16921 atattcaggt atgtcttcta aattaacaat tatttgttca tcatcacctt caccttttcc
    16981 agttaagtta tctccagtgt gtataatagc tttagaaggg tgtttaagat ttccaaaata
    17041 aaccaagtca cctctaacat tcattttatc ttggttagtc aatgctattg caaatgcatc
    17101 taggtcataa ttttttttac ccaatttatt catatcccat cctaagccta ctactagatt
    17161 ttttacagga ttattatctt ttactaagct aactctttgt cctttttgta aactaacact
    17221 cattttaagt tctccttttt atatttttag ttttattgat ggtatcaagt ataagaacca
    17281 tattagctat attatatacg tatttaggct aagttggcta tatttataaa ttatttatta
    17341 tataactaca tgaatatttt ataatttaaa catattgaca ttatatgatt taaattattt
    17401 tgtagtttta taatatttaa atttgtattt gattgttata aagtaaagta agtaataaag
    17461 ttagactcaa taattctaaa atttctaagc acactatcta aatgagaacg tatttggaaa
    17521 tacttgtata aaacaataat tgatacattt aatttttatc ttaaaaataa caattataat
    17581 atataaatag aaagtataat atataaatag caagtataat ataacatata aaaagttgaa
    17641 tttgtatatg ttaattatac ttgctattta ttgttagtta ttctattttt ttaaatccat
    17701 tacctaaagc ttcatgtata tctgtaatta taataaaagc attagggtcg acatttttta
    17761 ctaatttttt taattcgaca acttctttct tttcaactac tactaaaagt acatttttat
    17821 tttctccagt atatgcacct tctcctttta gaatagttgc acctctatct aaatcttcca
    17881 ttattttttt acttatatct tttgaaaggt cagatataat agtgaatgat ttggaataat
    17941 taaatccttc aataagcaag tcactaactt ttactaaaat gtataaagcc acagttgaat
    18001 aaagtgcaat ttcaacctgt ctatttacta tacctgataa aacaactaca acgcagtcta
    18061 caatacccat taaaacaggt atactaaatg taggaaaata tttatttagt aggagtgcca
    18121 ttaaatcagt tccacctgta gagccattta ttctaaaaat aagtccttga ccaactccca
    18181 ttaaaataga accagatata attgcaagat acatatcatt tgttatatct aatgtagcca
    18241 tatttgcagt aagcttaagt gctatggtaa gaaaaattat tcctaaaacc gttttaaaac
    18301 aatctttttt tgaaagtatt ttgtaagcaa atatgaataa tggaacattt aaaagtaaat
    18361 tgacaatcca aagttctaca ccgaataatg aatttaaaac aactccaagt cctgttatac
    18421 caccagcagc gattgcatgt ggattaaaaa ataaattaag agaaatggcc attaatatac
    18481 agcctacaat taatattaaa atttctgaaa aagttattct atcttttttc aataacatca
    18541 ctccttatat atagattata atatatatta tatagttttt ccaattttta ataaaagaaa
    18601 taaaaatata ttataaaaac aatttgattg gaattttcag aaaattaatg ctataattaa
    18661 aagtaaattt aaagggaggt tataaaatgg ctaaattaac tctagaacat ataaaaggaa
    18721 atacatatta tattccactc cctacaatag taggtgtata cgtagatggt aaagatgcaa
    18781 ttttaattga cagtggaaat aataaggata cagctagaca agttttaaga ttattagaag
    18841 aacataattt aagtcctaaa ttaataataa acactcattc aaatgcagac catataggag
    18901 gaaatgcata tttgaagaat caaacaaaat gcaagattgc tacgactaaa attgaaggat
    18961 attttacaga aaatccaata ttagaatcag catttttata tggtggctat ccaagtaaag
    19021 cactgaagaa taaattttta ttagcaaagg aaagtgaagt tgattatatt ataccttcaa
    19081 atggcaaaat tgtagataca gaacttgaag cgatttctct tccaggtcat tattttgaaa
    19141 tgattggtgt tagaactcca gataatgttt tgtttgttgc agatagtctt acaccagaga
    19201 atataattac taaatatcat tttttcttcc tactggatat agattcacag tttaagacac
    19261 tagaaaaatt gagaatgtta gaggctgatt tctttgttcc aagtcactct gtcaaaacta
    19321 cagatataaa aagtttgatt aatattaata agaagaagat ggaagaaata atagataata
    19381 ttaaaaaggt ttgttatgag ccagttatga ttgatgaagt tattgaaaaa atgtgtgatt
    19441 tgtacaatgt caagcttgat gctaatcaat atgtgttggt tggaagtaca atacgttctt
    19501 atataactta tttatatgag aataatatgg ttgaatatat atttgatggt ggtaagatga
    19561 tgataaaagc tgtgtaaata g
//
LOCUS       DAEPOZ010000047        19573 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.047, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000047 DAEPOZ010000000
VERSION     DAEPOZ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19573
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.047"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     repeat_region   28..586
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=93..121
                     /rpt_unit_seq="ctttacattccatatagttaatataaaac"
     gene            complement(1417..2289)
                     /locus_tag="KR440_001338"
     CDS             complement(1417..2289)
                     /locus_tag="KR440_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH0698395.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
     gene            complement(2683..3261)
                     /locus_tag="KR440_001339"
     CDS             complement(2683..3261)
                     /locus_tag="KR440_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBH0698396.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            complement(3319..4035)
                     /locus_tag="KR440_001340"
     CDS             complement(3319..4035)
                     /locus_tag="KR440_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBH0698397.1"
                     /translation="MRINKYIASCGIASRRKSEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKTYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            4226..5311
                     /locus_tag="KR440_001341"
     CDS             4226..5311
                     /locus_tag="KR440_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBH0698398.1"
                     /translation="MIIILKENIKYYLIVVFIGILFFKFINTPSNFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEVKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIDFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            complement(5466..6125)
                     /locus_tag="KR440_001342"
     CDS             complement(5466..6125)
                     /locus_tag="KR440_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698399.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KYSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(6342..7634)
                     /gene="lysA"
                     /locus_tag="KR440_001343"
     CDS             complement(6342..7634)
                     /gene="lysA"
                     /locus_tag="KR440_001343"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBH0698400.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYIEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            complement(7652..8845)
                     /locus_tag="KR440_001344"
     CDS             complement(7652..8845)
                     /locus_tag="KR440_001344"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBH0698401.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            complement(9206..9823)
                     /locus_tag="KR440_001345"
     CDS             complement(9206..9823)
                     /locus_tag="KR440_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698402.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            complement(9993..11693)
                     /locus_tag="KR440_001346"
     CDS             complement(9993..11693)
                     /locus_tag="KR440_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698403.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            complement(12090..12653)
                     /locus_tag="KR440_001347"
     CDS             complement(12090..12653)
                     /locus_tag="KR440_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698404.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            complement(12812..13525)
                     /locus_tag="KR440_001348"
     CDS             complement(12812..13525)
                     /locus_tag="KR440_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HBH0698405.1"
                     /translation="MGKVAKILLSLIVIVLVLAGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            13817..15481
                     /locus_tag="KR440_001349"
     CDS             13817..15481
                     /locus_tag="KR440_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HBH0698406.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDSKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(15588..16487)
                     /locus_tag="KR440_001350"
     CDS             complement(15588..16487)
                     /locus_tag="KR440_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBH0698407.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(16514..17152)
                     /locus_tag="KR440_001351"
     CDS             complement(16514..17152)
                     /locus_tag="KR440_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBH0698408.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KVGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(17489..18487)
                     /locus_tag="KR440_001352"
     CDS             complement(17489..18487)
                     /locus_tag="KR440_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HBH0698409.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(18585..19160)
                     /locus_tag="KR440_001353"
     CDS             complement(18585..19160)
                     /locus_tag="KR440_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698410.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
BASE COUNT         6400 a         3251 c         2039 g         7883 t
ORIGIN      
        1 gtgtatgaaa gagaaaacac aattttaatt tacattccat atagttaata taaaacagtt
       61 gtttttattt tttctaaaga gaaaaaacta gactttacat tccatatagt taatataaaa
      121 ctttctgtat attttagttg tgttttaata ttataacact ttacattcca tatagttaat
      181 ataaaacatg gcagtaacta agaagaaaac tatacctttt ttattcttta cattccatat
      241 agttaatata aaacgaggaa attttatact aatgactgat gctagattta actttacatt
      301 ccatatagtt aatataaaac tattataagg agcattatac gaccaaaagc caaagcagct
      361 ttacattcca tatagttaat ataaaacaag caacagcaag agtcgcgaca acattaaaaa
      421 cttcctttac attccatata gttaatataa aacctttatt tttttctcta tatcgagttc
      481 gccaaattca ctctttacat tccatatagt taatataaaa cagtccagcg ttgtgtggtc
      541 gtaacacacc aatcctactt tacattccat atagttaata taaaacccca aaataaatta
      601 agtatttcca atacttatac atatacaatt ctcctaaact tgcagtgagc gagcaatagt
      661 gcaattgata acagttatca tacactctca acgctttata tttcaactat taaaccctac
      721 tttatcacaa aaatcgctca ctgcaaaact tttatatttt tattatatca taaaactcat
      781 actataaaat aaaaatggga taattttaca aactaaaagc atctacattc ttataagtat
      841 ttattatatt ctctattcca atgattcctt tttcttttta ttgcatcctt cctatgtaat
      901 cgcactttag ctcaacaagt cttttatttt cattaacaat tttttaaact attcaaaatc
      961 atcaaatttt tcatcttata cagttaaatc aaaatacgtg ttatatctct ttatctatat
     1021 acttttcttc cttccatttc tttttctctt tctacatttt cactttgtta ctttacaatc
     1081 tcaaggttat tatctgctat taaagtttta tctttccagg tttcacaaaa ataaattgaa
     1141 taagtgcaaa aataacagat acaaaaccat aactattttc ttttctataa aaaaatatat
     1201 catcaacaag aaacataata aatgaaaata actatgaata aatactctca tttagaagaa
     1261 atagacaaag atattcctta aaatttaagc aaaatattgt ttaaataaaa aaactgactg
     1321 tcagttaaat gccagtcagc tattttatat attttatcca aacatcacta tatttgaaca
     1381 ttttaataat taataatact tattaatata caaactttac tcatacacag aatatctttt
     1441 ccaaatttgc tctaaatcat caaaatgctg tttaatatct tctttttcct tcattccaac
     1501 agatatagca agggcatttg aaatactaag agctggaact agagaatcta caaatgaagc
     1561 catattactc ttaactagaa gagtgttatc tgcaagtgat gctactggcg caaataagct
     1621 atctgttaat gatattacat gggctccttt ttctttagca taattgacaa tttgataaga
     1681 tttcttagaa taacgaggga aactaatagc aactattaca tcttcttcat ttattcttac
     1741 aatctgctca aaagcatctc ccatatccat tctaattata tgaacattgt caagtattat
     1801 atctaagtaa aaacctaaat attgagctac tacaaaagaa ctcctcattc caagtatata
     1861 tatttttctc gctcttaaaa gtttgttaga agcttcttta aacgctctct cgtcaatttc
     1921 ttctagtgta gaccttatat tatctatatc actttttaaa actttgttta gtattgcaaa
     1981 atcatcggaa taatcatgtg ccatttcaac tctttgtact gtagttagtt tattttttat
     2041 gagttcttga agtgcagctt gtaatttagg atatccacta taacctaatg catttgcaaa
     2101 tcttacaact gtagactcac ttactcctac ggtttcaccc aatttacatg cagtcataaa
     2161 tgcaacttta tcataattat ttaaaatgta ttgtgctata agtttttgac ccttactcaa
     2221 tctcgtatat tgagattgta tatttgatat caaatgttta ctgtccttca tttcatttgt
     2281 ttcttccatt tttttctcct tttagttata taaactattt tgttttacta acactgtctt
     2341 tttctctaaa aattttattt tttatctcaa tttcatctta gtttttatca tagcttctta
     2401 aactcataaa attctaactt ttctgtacct tatacttcac atagtatttt cgattaaatt
     2461 tattatacca tctttccttg cattttttaa tatttatcca atatttcttt taaatttttc
     2521 aatttttact catttaaaag ttatctatct tattttgcat atttagatgt atattttgtt
     2581 ttgagaataa gctgttgtat acttattatt taaagtattt ttaataggtc acactatata
     2641 tattaactct taatagaatc ttgtcaagtt ttttagtatt tattatgctt ttttcttttc
     2701 aattaaaatc aattgtggtg ggttattgac ttgatttaaa aacttagatt ctaaaacatt
     2761 aaattcatct tgatttaaat tattgacaag gttatatatg caatcttttt cttcttgacc
     2821 tccatcatgc cctgtatata tggcaatgct tattactcca ttttctttta acatttctaa
     2881 acttaacttt atagccttta aagttgtatc tggtttggtt ataattgaat gtttcgctct
     2941 tggaagataa cccaaattaa ataaaacaca tgaaacttct tcttttatat attgattcat
     3001 tttttcatgc ccatcaaata tcaactttac tctatctgta tatccttctt tctctagttt
     3061 ctttcttgta gactttattg cttcttcttg tatatcaaaa gcatatacaa agcccttttc
     3121 tccaaccttc tcagctagat accttgtatc atacccattt cccatagtag catcaattac
     3181 tatgtcccct tcatttataa tattttctaa ataaaattta tttatatctg taattttagt
     3241 taaatacttt gcatatgcca taatactatt tctccctact attaacttta tttcattttt
     3301 tctcaattta tttttatctt attttattga ttttaaatat tttatttcat cttctgttag
     3361 gtatctatat tctccaactt tggtgtcttt taatactatt ttccccacag aaatccttct
     3421 taaatttaaa actggatgat ttatagcttt gcacattttt cttacttgtc tatttctacc
     3481 ctcatgtata gttatttcac acactgaata atttttttct ttattttctt tagttaccct
     3541 tattttggca ggtgccgttg tgtaatcttc tatttttagt cctgtttcga attgtcttat
     3601 ctcatcatca cttataattc ccttcacact tgccacatac gttttatcaa tttcatgctt
     3661 tggatgggta agcttatatg ttaaatcgcc atcattagta agtattaata aaccactagt
     3721 ttcataatct aatctcccta ttggatatac tctttccctt acatctttaa gtatgtctat
     3781 tacactgggt ctattgaatt ggtcttttac tgtagttata tatccctctg gtttatttaa
     3841 tactatatat atataatttt cacttggttt tactttttta ttatcaaatt ctactatgtc
     3901 tttttcttca tcaatattaa aagataactc ttttactaca cttccattta cctttattct
     3961 tcctccaaga attatttctt ctgattttct tcttgatgct attccacaag aagctatata
     4021 cttattaatt ctcatactta atccttcctt atcttaaatt atgtgtgttt aatattttta
     4081 tcaatgttaa ttttgctaca ttattatact atactagacc ttaattaaaa acaagcaaaa
     4141 aaattattta attttcctaa ataattgtaa tttcttttca catactatta atatgtaata
     4201 gctgactaaa cactatttta aggaagtgat tattatttta aaggaaaata taaaatatta
     4261 tttaatagtc gtgtttattg gaattttatt ttttaaattt attaatactc catcaaactt
     4321 tatatctagt attgaaggat tcttaaagtt ttttagccca tttttattag ctattttact
     4381 tgctttactt ttaaatccac ttgtgatgct ttttgaggta aaattcaaag cacatagact
     4441 tttagccatt tttttatctt atatatttat aggttttatt ttagcctttg ccattaggct
     4501 attaattcca tcaatagcta atacattaaa tagattgata aatgaaatgc ctatgtatac
     4561 tgattatata gatagtttta tagaaaaaaa tatgtcaaac atagattttc taaaaacttt
     4621 aattcctcat atacaacata gtttagataa tttattaaaa gaagcaagta attttgttgg
     4681 taaaattcct aaaaacttct taatctatac acttagtata tcttctatgc tatttaatat
     4741 gacaatggga tttatactat ccatatacat tatatatgac aaagaaaaaa ttgcattggg
     4801 atttaagaga tttctatatt cttctactgc tagaaacaag gcagataaca ttatagaatt
     4861 ttttaggact actcatgata ttttctatga ttatttactt ggtagaatat tagactctct
     4921 tattattggt ataattgcat ttttaggatt tcaatttgtt atcagaatag aaaatgcttt
     4981 atttttagct tcaataatat tcttaggtaa tataattccc tattttggtc cttttattgg
     5041 tgcaatccct ccaatagcca tgactatatt atatagtcca caaaaaacta tatgggtcat
     5101 tgcatttcta tttatattgc aacaattaga tggaaatttt atagaaccaa aagttatggg
     5161 taatcaagtt ggtataggag ctatatgggt aatatctgct attttggtgg gacaatctct
     5221 atttggtttc ataggagttt ttctttcagt accaatagct gctgttgtaa aaacttatgt
     5281 tgataaatat atagataatc gtttacaata gcttatctct actgtgactt taatttttag
     5341 tttaaaattt aactgttatt tattaaatta tttctcaaaa aatatctctt agtaagaaaa
     5401 aattgctaag agatattttt tataatatat aatttgttca ttaaatttat gcatatattt
     5461 tatacttatt gattatttga gttttgagaa tttccaccac tatctgaacc accagtagta
     5521 tcttgtggag ttggtgctgg tgttggatta ctattatttg gcttttcttg attttgagaa
     5581 gaactgttat tattgtcaga cttattacta tctgattttt tatcattatt tttagaacta
     5641 tatttattat ttttactact tccactataa gaagatttcc ttgcatctga ttcaactcta
     5701 tacctatctt ctccagccgt atcttttgcc tttttctttc tttgttcctc tgcttcgttt
     5761 tgttcttttt ctaatttagt taactcttct ttctttttag ttgcctcttc tttaataaca
     5821 gaggaaccat ttttgatttt ctctataagt ttaagttcct tgattgcacc ctcataattt
     5881 tttaattctt caaactccgt catatttgac attctcatag cttgcatata catagctctt
     5941 gcattctcat tagtactatc ttcttccagt gcagaggata atgattccat agctttttta
     6001 tagttatgtt tttctaaata tgtttttcct tgctctacta aatttatttc atctttttta
     6061 ttaaatgaac atcctacaag aaatatagat aatatcgata ttaataaaat tactcttcct
     6121 ctcattaaat ccctccttgt catatacttt aaattttatc ataattataa agtttttaaa
     6181 atattttata tttattacaa gaaatttgtt agtaaaacaa aaaataagca gatatgctac
     6241 gaacgataca ataaaccaaa aattaattta gagatacata tcctagcaaa tctgctcata
     6301 ataaacttta atatttaatt tttattatat acttttccat tttatatctc taaagctaat
     6361 aagtcctcat agctttgtct cttacaaatt atcttatcat ttccatcaaa tactgatact
     6421 actgctgctc ttggtatttt attataattt gaagacattg aatatccata tgcaccagtt
     6481 gatgttgtta taagtatatc tccactttta atatccatta tgtttatatc tccaattaat
     6541 atatctccac tctcacaaca ttttccagct attgttacat gattcatagc ctcatgatta
     6601 attttattca caatactaca ctcataacta gcttggtaca acgaaggtct tacattatct
     6661 gtcatacctc catctacact tacataaacc ctaacatctt ttatatcttt tatagaacct
     6721 actgtataca atgtacttcc tgcatttgca actattgatc ttcctggttc tattaccaat
     6781 gtaggtactt ctattccaag ctctttacat gaagtttctg ctttagtgat tatggtttca
     6841 caaaactctt ttatagtttt aggtttatct ccttcgctat aataaacgcc aactcctcca
     6901 cctaagtcaa tttctgttag ttgtatgttg aatttttctt ttatttcttt taccaagttc
     6961 atcattatat ccactgtatc tatgtatggt tctacatcaa atatttgtga acctatatga
     7021 gcgtgaagac ctacaagttt aatattttta tattcactta atttttctat tgctctaaat
     7081 aaatctccat ttactaaagc aaatccaaat ttagaatcta tctgtccagt ttttatataa
     7141 tcatgtgtat gagcttctat accaggagtt attctaaagt atatctcttg agttttacct
     7201 ttatcttcac ataatgcttc aagcaaatct aattcataaa aattatccac tacaaattta
     7261 cctacaccta aatctaatcc catgtttatt tcttctttag tcttattgtt tccatgaaag
     7321 aaaacttttt ccataggaaa atttgattta taagctgtat ataactcgcc tcctgaaact
     7381 acatctaaac acatttgttc ttcatttata agattacaca tgtaaagagg taaaaaagct
     7441 ttccctgcat aagcaacctt attttttccc tctctagcct taaaatattc tatatattct
     7501 ctacaatttt gtctaactaa agcttcatca aatacataaa gtggagtttg atatttttta
     7561 ctaagttcta aacaactcac tcctccaata tataattcat cattatttac agtcatagtt
     7621 ccatgaagtt tcataatagc ctccccaaat tttatatatt aaatacttct gctatttttt
     7681 ctatagctac ttgcttatca tcagaattta tagcacaagt tatacgaatc tctgatgtag
     7741 ttataagttt tatcattata ccattttcat tgaatatctt aaatacttta gcagcaactc
     7801 cagaagtatt tttcatacca agacctacta aagaaaattt agtgatatta ttatcaatca
     7861 ctacatgttc ttcatctgta tatttagata ctatcttttt acattctccc aactcttctt
     7921 ttggaactgt aaatgataca cttatcttat ctaaaatagg tgcagtttgg cttatcatat
     7981 ctacacttat acctatagtt gctatatctt caaataagct agatatattt tcagctttaa
     8041 aatcttttat tgttatagaa ctgtcatcat cactagtagc taatcctgtt attactttat
     8101 cctctaattt catgtctttt ccccctctta tatatgttcc tttttctgta ccacatgtac
     8161 gtccgacata gatttcaaca ccatattttt gtgcaagctc tatacttctt gaatgcatta
     8221 cttgtgctcc taaactcgct aactcaagca tttcttcata ttcaatctca tcaagtttac
     8281 tagcctttga atattttctt gggtctgtaa aataaattcc atcaacatct gtatatatct
     8341 cacattttcc atttaattta accgcaagag ctacagcaga cgtatctgaa ccacctcttc
     8401 caagagtggt tacatcacca tcttcgttga ttccctgaaa tccagttact atcacgacat
     8461 ttccttcatc taaactattt ttaattctgt ttacgtttat atcatcaatt tgagactttc
     8521 catgaagacc actcgttttt atatttaact gataagcatt ataacttata gctttacatc
     8581 caagtgcatt taatgctatt gataataaag atgctgaaat catttcacca gtagacatta
     8641 aagcatctaa ttccctctta ctaggttcat tactcaattc ctttgctaaa gttatatatt
     8701 catcagtgga ttttcccatg gcagacacaa ctattaccat ttgtggattt tcttttcttc
     8761 gttctattat attttctgct attgatttta ttttttcagt atctgctact gaacttccac
     8821 catatttttg aacaattata ctcatgcttt ctccccctca agctaaattt tgataaataa
     8881 aaacgcccaa aggtatactt tggacgtgta aatttcatag accaagtaac cttgtagctc
     8941 accactttaa aagtgacagc cttatgcata ttctgcataa gtccagaaaa tttgtccgtc
     9001 aacaaaattt cttcggctat ataccctttc atcaattatc aatgcttacc agctcctatt
     9061 gaatactatt gaatacagct acctctaccc taggctcatt atttttaatt aatttattaa
     9121 tatagtttta tcatattcta tataatattg caacaattat tttacaaaat gtggcaaatc
     9181 gatagttttc ttttctaaag ttagcttata tacaattaaa tagtttattt aatcctaaat
     9241 ctataaaatt tatgctatac tcattatcat atctctctaa atcatttaat tcggatagat
     9301 tatctggtgt aactaccaaa acgtttggta ctttaaaaaa tggaattgta gatttatcta
     9361 tttttttaga aagattttcg tatttagaaa tgtctatttc attatttaag tcaacatcaa
     9421 ctaaaacttg ctttatatta ccaaattggt ctgtatattc aataaataaa tcgtatttca
     9481 catcatctac actaacattt cggtaaaatc tttttacatc acaacctgca gtattgattc
     9541 taacaactaa ttctgatcct actagagcct ttattaaatc atctccctta aaaagtttac
     9601 taccgtttaa ataatataca taagagaggt ctttataatt ataataatcc acatcacaag
     9661 gatatttttt tagtgtatat tcactacaca tttttctcaa agttttttta aaattttttt
     9721 tattgtatct tttttggtct ccataaattt ttctaaattg agactctgta acacatctaa
     9781 ccctgctaat taaaagtaat atctcttttt ccatattcgt catagtacat tctccttgaa
     9841 gtattatttg caaagttgta ctaatattat attaatttta tgacattaaa tttacaaaga
     9901 attaaaataa aatagctaat ttttatagaa ttaaattcta taaaaattag ctatcactat
     9961 gttaaatact attatcaatc tttttattta aattatacat taccttgatt ttcagaatct
    10021 ttggaatctg tattttcatc agttcctgtt ttttcttttg cttttttatc ttcaaagttt
    10081 gcaatattta ggttatcaaa ttctgacatt tctattacac cagattcttc ttttgaatac
    10141 gatattttga ctttatcacc aacctgagtc aatggcaatt cgctagatac ttttgaagta
    10201 gccctaaata ttatatcttt atttgaatct aaagttacat aataattagt gttaccacct
    10261 cgcacatctg ggcttattct tgtcactgtg ccttctaata cctcatcttt taaatcatta
    10321 tcaagtttta aatcattacc cttagattct aatgcatcct tatagtttct taatgcttca
    10381 ttcttatctt cacctattcc aagtacatta taatcttcaa cagatacaaa tgcaactctc
    10441 ttaacaagac cagccttatc ttttaatgat gatacatatg ttggctgacc caatatatta
    10501 tacattacag ggaacgtcgc ttcataattt ttttcttgaa cattaccttc agctgatttc
    10561 attgcagccg tttcagtagc tcctggttgt ttatataatc tagcttcttt tgttctagaa
    10621 tcaactaaca taaatccaat tgtagattca tctccacctg aagatgttat acctgtatac
    10681 caataagacc tgttatcatt gccataaact aatgatgtac cttctgttgg tactaaaact
    10741 cccttttctg atataacaga gtttaaaaat ccattaacat aaacacccca gtctttaatc
    10801 tggtctgtta caaagctttg tggttgtatt ctatccaccc atttaggtgt gtttttaaca
    10861 tcataaacat ttatttttcc agtttctgca tcaacagtgg ctattcctgt tgcatttgct
    10921 cctccatagc caattttatg ttcatataaa cttacaaccc aataaggtct tccttcatca
    10981 tttatttcaa gagtgaaatc tgtcatacca acatttacaa taccatgtat gtataaatgt
    11041 ctttgtaaat cctgatgtaa gtaggcttct ggttggtaaa ctatctttat tggctttcca
    11101 tctatttctt gaactaattg gacgtcttga ggattactag ctgatacttt tacataacca
    11161 cttgtaccac ttagagatgt tatccattta attatgtctc tgtgaacaag aggcgctacc
    11221 cagtacaact ctccatctac attttgtata tgaaattttc ctaatttgga tacactccct
    11281 attgcaggaa cttctccaag ctttttgtca cctaatttca ttgccatatc ttcatctaca
    11341 agacgaatgt catttacact gacagggcta atgtcatcag taaatttact ttcagttact
    11401 tttcccaata gttctctata tgattttgca tgaagtacag gtgtagaagt tataaaggga
    11461 actactacaa tatacacaag caaagctatt gctattgaaa aattatattt agccatttta
    11521 gatgctcttt catttctatc taacatcata tctagtattc ctgctacaac aaagaaaatt
    11581 attcctactg aaaataaaga tgtaaaatct aatcttagta caggtaattt aataaatgca
    11641 taaattatta caattattaa tgcaactcca tatgttttaa gatatcgttt catttaatac
    11701 tcctctctaa aaaatgttat tattattatt atatccatat atttacaaaa aataaatatt
    11761 tatcatattt attatatatt aagatatctt tatatattta tagttctgct tattcaacta
    11821 tatttaacat tatacgtgta tgtataaata ctttttatac actgtttgtg ctatccatcc
    11881 cgcacctaaa ggagtgggat ttttgcacta ttctgtatta tatatatcca aaaatattat
    11941 aaaaatacaa atattagcag aaatttaaaa actgaagaaa gtattctaac taagatttac
    12001 aaattcttaa tcctatcgaa gttaaactac tatcttcagc ctaattatct agcaatattt
    12061 ttttatatga aaaactctaa atcaagtttt taaatgtttg cgatttttga tacataagaa
    12121 tgttgtctag aattatattc tgataataaa ccttcatact ctaaggttaa gccagataaa
    12181 ttataaaaat catttggcgt tacttcaaat gcttgtttat ttaaaatgtc tatctcactt
    12241 ttttctaaca tgtcagcaac aaagtttgaa catacatatt tatattcaga ttgtctctga
    12301 atattcatta gtaaatatat taatgccatt gtatcgtaat agtaatcttc tctataatca
    12361 ctaattaatt ttatattttt atatagattt tcatattgta aattatctac ttctaacgaa
    12421 tataccctac acacagtgtt ttttcttata gcatacaatc cttgatttat attttcttca
    12481 acaaatcctc caataaatgg attccttgga tttagccttc caaaagaata cattggtttt
    12541 agattttcat ttaatgatat tgacacatgt gtatacagct ttttacttat attccttatt
    12601 aagtaagata atatagttcc tgtatatgtt gttacaatat agactttatt catcatttcc
    12661 ccccaatcta gtaactaaat gatattatac acttacaatt taatatcata agatattcta
    12721 acataataaa tgtggtttta caaattttga ttttaaggta ctttaatatt atataatata
    12781 ataaaaatat aaatataagt tttctatatt tttaaccaac agatgttttc ttcaaattaa
    12841 tcagatgatt tagtatatct tgaacctttc cagagtcctt taaatatcct aatgccccat
    12901 ttatttgtga ttttaaatct atttctactt ccatatccaa atcaacattt ccttttttta
    12961 tagatatatt tttaagtgtt attgggtaaa gataactttt ttccaaagta atcttatttc
    13021 cattaacctc aaatggaact tttttattgt agttacttaa tattttttct agcattttat
    13081 cacttagttt aaattttcca attttcacat tctcaagact tacaactaag tttccatcct
    13141 ttaaagtagg tctcaattca agttcatatt gactatttac caaaccaaat accttatatg
    13201 gtcctgctac ctttatttta ccatttttca tttcaacaaa attattttca agttcattaa
    13261 ttccatgctt ctgcatcaat gtatatatta aatttttaaa ctcttcctct gaaacagata
    13321 tttttccaac cattctaagt ggatttttta ctacctccat agaatttata tatgagcctt
    13381 ttgataaata ttcttcatct atgattttat tattttgtga actaacatca tatctttctt
    13441 ttggtgtcaa gatatataca acaattcctg caagtactaa aacaataaca attaaactta
    13501 acaaaatctt tgcaaccttt cccataatcg tctcctttac aaatctgtca tcagatttaa
    13561 aataaattta gataactaac ttcacataat attcttaaca atatacatac tataatacag
    13621 tcttttaaaa attaattcaa ccaatctaat gttttgtaaa taaactgtaa aaacatgttt
    13681 ttagtataaa agttaatatt ttgcataata ctcttaatta cacctttatt tttttctaat
    13741 cttggattaa aattaatatt atgcaataca gtgtagtgtt ctgtattata atcaaattaa
    13801 ataggaggta tagaatatgt caaatgaaac gaactcatct aactttataa agaatattat
    13861 tataaacgac ttagaaacag gaaaacatga cagtatcata actcgttttc cacctgagcc
    13921 aaacggatat ttacatattg gtcatgcaaa aagtatatgt cttaattttg gattagctaa
    13981 ggagtttaat ggaaaagcta atttaagatt tgatgataca aacccattaa aagaggatgt
    14041 tgaatatgta gagtccataa aagaagatgt aaagtggtta ggatttgatt ggaacgagtt
    14101 aaattttgca tctaactact ttgatgaaat gtataagaga gctttaatac taataaaaaa
    14161 gggtaaagct tatgtatgtg atttgacaca agaggaaatg agagaatatc gtggtactct
    14221 aactgaacca ggaaaagaaa gtcctcatag aaacagaact attgaagaaa accttgattt
    14281 atttgaaaga atgaaaaatg gtgaatttaa agatggtgaa aaaacattaa gagccaaaat
    14341 agatatgtct tctccaaata taaaccttag agacccaatt atatatagaa tatctcattc
    14401 aacacaccat aatacaggag ataaatggtg catatatcct atgtatgctt ttgctcatcc
    14461 aattgaagat gcaattgaag gaataactca ctctatatgt actttagagt ttgaagacca
    14521 aagacctctt tatgattggt ttgttaaaga atgtgagatg gaaaatatac ctagacaaat
    14581 agaatttgct agacttaata taaataatac tgtcatgagt aaaaggaaat taaaacaact
    14641 tgtagatgag ggtattgttg atggttggga tgaccctcgt gttcctacaa tatccggaat
    14701 cagacgtaag ggatatactg cagaagcatt gcgcaatttc tgtagtgaaa taggggtatc
    14761 aaaagttaac tctacggtag atagtcaaat gcttgattat ttcctaagag aaaacttaca
    14821 accaaaggct cctcttacta tgggagtttt aagaccttta aaacttatta taactaatta
    14881 cccagaagat aagattgaga tgcttgaaat agagaataat gctaaagatg aatctcaagg
    14941 aaagagatta gttcctttct ctagagaatt gtatatagaa caagatgatt ttatggaaga
    15001 acctgttaag aaatatttta gatttttccc aggaaatgaa gtccgtttaa aaggtgctta
    15061 ttttgtaaaa tgtactgatg taattaagga tgaaaatgga aatgtagttg aaatacatgg
    15121 tacttatgac cctgaaacta agagtggctc tggctttaca ggacgtaagg taaaatctac
    15181 aatacattgg gttgatgcta aatcagctat accatgtgaa tttagattat ttgaaccatt
    15241 aattcttgat gatatacctg aaaatgaagg taaacatttc ttagaacaaa taaatcctaa
    15301 ttcattggaa atattacaag gttttgttga acctacacag ataaaagatt ctaaaccttt
    15361 tgataaattc caatttgtta gaaatggatt ctttagtata gataataaat atacaactga
    15421 cgaaaaattt gtatttaata gaatagttcc tctaaaaagt tcttttaaac caggtaaata
    15481 atcttacaat caaaaaatga agacttaaaa tataaattac ttctactata aataaactta
    15541 gttagaagta attttttatt tgagtcttca tcatttactt taaatgatta acttttttca
    15601 ctacagattc ccataacttc tattccatct tttatgcatt ctatgttaag aggaaaatct
    15661 ggtccttttt ctccgtcaat atctgttata agtgaatctt cacattctat atgcaccttt
    15721 ttagttttaa agtatagtat ttcatttcca ttatattgat ctaaataatc acctctaagc
    15781 acacttatca aaactggtat tgattttggt attggcattc ctttaaaaat aattacatcc
    15841 aaatatccat catctatttc agccttatat gctagattta tatttcctgc tgttttgcca
    15901 ttaaaaatca acataagata catatctcca tcataataca tttcctcaga agatactttt
    15961 atcttaaatt tcctcatata caatgcttct tctattccct ttatataata agaaattctt
    16021 ccaaatgaat tttttaagtc tgtgtttatc ctttgtgata catctgtaaa cataccagca
    16081 cttgcaacat ttataaagta cttattattg actttcccta tgtctatttt ctttggtttt
    16141 gaatttatga tttcatttat agcttctttt atatcaaatg aaatttgaag agcatttgaa
    16201 aaatcatttg cagttcctgt aggcaaaatt ccaattggaa tttttatgtc taattctttc
    16261 attgcattta ctactaaatc tacagtacca tctccaccag ctatcaatat gtgttcataa
    16321 gaactatcaa cttccttaaa tgcattcttt acaggttctt taatagttaa tctatagggt
    16381 atcaataaat aatttttttc ttgatataat tctatgatag tgtctaaatt tgataatatc
    16441 tttttttcac ctgaattagg attgtatata agttttactt ttttcattat ttctccttac
    16501 tcattttatt cttttagtat ccaaaatctt catttaaaaa aatcacatgt attctttctg
    16561 gtgttaattg tctttttata agtgtataac tacagcatat tcttcctgga cttgaacagt
    16621 ctatacatat accagtagtc ttacatccag tatttgtact taatctttta caattggctg
    16681 gagcagctat tgtttttgtt ctttttatag cttcatctac atctttaact attttattta
    16741 ctcctgctat aactattact ttatcaggtc catatatcat agcagcaact ctattcccat
    16801 tcccatctaa attgtaaagt tctccatcct ctgttatcgc attagtactt gtaaaataag
    16861 catctgcaga aaatgacttt ctatatattt ctttcatttc ttctggtttg atatcttttg
    16921 catatctatc aagaaaatta tatttttctc ttataaaatc aatcactcct gtttcaaata
    16981 aggtcataga acctccacat cctactagag aacctacttt aactatttct tcaatttttt
    17041 taagaagctc ttctttgtta cttactaaat accctttcat attattgttt tccagagatt
    17101 ttattgtttt ttctattctt ttttcattaa gccaatttaa attttgatcc attttaactt
    17161 cccccttatt tttaatatgt acttaattat ttataaactt tattgtcttt ataagtttat
    17221 tttagtacat ctaaaaaaaa atacaatata aaacttaaat agttttatat aattttttcc
    17281 aaaataactt actcaatcaa aaaaggaaag tagatgtact attctacttt ccctatattg
    17341 tatgtagtat ttattgccta aaatctatat attaattata tgtttcatat tatataatat
    17401 cacagttaat taaaatgtaa tactatcttc tttttaaaca tataaattgt attattaaat
    17461 ttaatatatc caacttcaat aaatcttatt aatttgatga agaagatgct atagctgcac
    17521 ttgttgctgc cattacacat gcaatttgaa tagcaacaac aatatttaaa aatatattat
    17581 tagttataga gcctatatta ttctcatttc gtatcaaatc tgcataaatt gaagctacaa
    17641 tagcagaact aatcattact ctattacttt ttcctaaaga ccaatttcca aagccttttt
    17701 cttctttaag tttatttgaa acatttttta tctcttctat tatttcatct tctctataat
    17761 ctaataatga aattgctcct attattggat atccataata attcatttta caatcatttt
    17821 ctttaaatgc ttttttaagt tcaatagtct ttttacattt aagcatagtt ctatcatcac
    17881 taaataaaag aatttgggat aatgcttgta aatcatttcc tttataaaaa ccattttcat
    17941 ttagaaattc ataacatttt tctatgtatt ctaaatcttc atctaaattt tttgaatgaa
    18001 ttgcaataag tgctatatta caatagtcat cactagaagt tagaaatgga tgttttttcc
    18061 tcatgtaatc atatattatt ttcatttttg atatacatgt aagataatcc attttctctc
    18121 tattttcata aactattgat gctgtaagtg gtaaatatgt atcgccccaa aacttttcac
    18181 ttttaagttc tttgtatata tctaaaatca tcttaaaact actttctgga tttgattcaa
    18241 acgaaagtag tgttgccata taaaacatat tatatcctct aaaattagag aataatccag
    18301 tatttttttt aataagctct attcgttctt ccactaatct aggacttatt ctcttgttct
    18361 taagagtaaa tgcaagtgca caactatgtt gtatcaatcc catttgccaa gaacctttaa
    18421 cttcatttaa tgctttgtaa ttttctatta ataagtttac ttttccattt ataaacgatt
    18481 gttccatatt gccctcctag taatatatct cattgtacta atagttttat atttattata
    18541 tatatattaa atactacaat caatacatat tacttcaaag aacatcattc ttcaacaata
    18601 atgtagcctt ttttgtaatg tcttcccata ttattgactc tatcatagta ctctgattca
    18661 ctttcattac tccatttggg cacaaataca tctattgcat tagaattact aaaatactta
    18721 ctagaccccc tatccataac agtttttaat tttccatcta caataatctt agttccaaaa
    18781 ctcaagtgat tgtttgctac aaaaccatct tctaaatcat ctcctgtagc agttatcgct
    18841 ccatatccat tttcttgatt aagacttgtg taataactaa cttcaaaagt tataggtact
    18901 aacttcttta ttttaatacc taactgattt tgtagtttct ccttttgtac tttcaactta
    18961 tactcatcac tatttttata atcttccata ccttgattat acttggaatt aaagctttta
    19021 cctgacttta ctatagtatc tatatttact atagattctt tacaatgatc tgcatctatt
    19081 tttgtttctc catttaccaa taatggagtt aaacatacac tacatacaac acatgtaatc
    19141 aatgcttctt taagcttcat aaattccccc cttattattt tgatatatta gcatatttaa
    19201 tattatatgt ctatatttag atttaaatac cacacccatt tgctttaaga agttgtctta
    19261 tatttaattc accttaaaca cttataaatc ctctatctta gaatcatata gaataaagct
    19321 tttatcgtta aatatttttt attaatcttt taaaatctaa agctacttta tttaattttc
    19381 tcttttttag aatttaagaa ttaaaattct ataattttaa aacatattga atttattttt
    19441 ttgagatgat ataattttct taagaggtgg ttgatatgat aaaaaataca aaacaaatta
    19501 cagtagaagt taatgaaatt ttattacaag aaatatctaa gataactaat gacgaaaata
    19561 atattttgac aga
//
LOCUS       DAEPOZ010000048        19217 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.048, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000048 DAEPOZ010000000
VERSION     DAEPOZ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19217
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.048"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     repeat_region   599..955
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=599..627
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            1194..1382
                     /locus_tag="KR440_001354"
     CDS             1194..1382
                     /locus_tag="KR440_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698411.1"
                     /translation="MKIITDSSRADYFKQRRQNKKTFSVLLDREKVEKIEEHLKKQNK
                     TKTIWLEEKINEELEKEE"
     gene            1501..2367
                     /locus_tag="KR440_001355"
     CDS             1501..2367
                     /locus_tag="KR440_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HBH0698412.1"
                     /translation="MKNLIVKEFNGSQIYTFMWKEKSCWIANQIVGLFDYADVSKTIQ
                     DCIKAEDFEIEQEYDVLKGNEFNDFVTTLNVVANNIISNKARSITIFYEDGLYGFLQY
                     TDKPIGVQFRKWLRREVLPSIRQTGAYITNNANPEKLREKASEIEKLQLAYNSTSMLK
                     ELLDGAGFDNKSKLLTAKTLYKKAGIDLPIEINEEEHYFDTKQIASKLKIYSKSNKPA
                     QLAVCEIIKKIDLEENEVKGVWETNGSWTGTVNKYTKSVIDKVRNWIEENNRPTKIAG
                     EKKNYHVVYKIE"
     gene            3232..3759
                     /locus_tag="KR440_001356"
     CDS             3232..3759
                     /locus_tag="KR440_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HBH0698413.1"
                     /translation="MREGKRRRGCLFWFILIILFSGVVGAIAGNSTNNESTEKQKEDL
                     TKYIGEEGSIGDLKLTVNSISKASEISVASGYLAYTPDSGKYGIINLTIKNQTKESQS
                     FMLNSFTLIGPDDSKYVPSLLIDVGNKYITMDTVNPNLDVTGNIAFEIPKDLLVSDCK
                     LKYSGSEQEFILKEK"
     gene            3897..4664
                     /locus_tag="KR440_001357"
     CDS             3897..4664
                     /locus_tag="KR440_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HBH0698414.1"
                     /translation="MGLFGKKKADICCICNTEIGVLNIEDGWICNSCFKEYCDALSMT
                     KAPKILRKLDIEKTISSTKKNNELQKIFNTTNNIENYIEFDEDNKKWLVSKKSINDKK
                     TPIIHSYEDIVKFELLENGETVTKGGIGRALAGGILFGEAGAIVGGITGKKTTRKVVD
                     TFKIKITINNIDNPIEYIELINKKTKTNSSAYEKAYKDAHKILSTLSAITQSIKETDN
                     INTKSVADEILKYKNLLDMEAITQDEFNTKKKELLNL"
     gene            4730..7102
                     /locus_tag="KR440_001358"
     CDS             4730..7102
                     /locus_tag="KR440_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBH0698415.1"
                     /translation="MATIQTSIKIFDGMTPAFRNMTTSINTTINSLERLQQRLHNPLN
                     TGGIQASQQSLNNIENILTRIEQKIGRNTNEQENFNNKIKQGSEAGSLLVSKLKSLAG
                     IYIGIRGIESITKAADTIASTKARLNLMNDGLQTTEQLNKMIYLSAQSARASYADTAA
                     QVAKLGILAGDAFGSSAEVIKFTELMNKAFVIGGTSANEASAAMYQLTQAMGAGKLQG
                     DEFRSIMENAPLLATKIADAMGKTKDQLKELSSSGAITADVIRNALFKASDEIEKKFA
                     SMPVTFSQALTMMKNDAYMIFGQTLGKISGALQSVRFSEIVVSIRNVMIAISSNIYDT
                     LNIIKNILNSEFFSNLVQGFTFCAVVITKSLGSIVNTALNIVNIFAQNWSIIKPIVFG
                     VASVFILFRGVLLATKIATIGNVIANVAHAASSSLSALMTNIQAAALMRSNGATLSAT
                     IATWGLNAALLACPITWVVLGILAFVVVVFVAVAAVNKFAGTSLTALGVIVGAVFAAV
                     AAIQNVMIWLLNGCIAVNEGITNGWNQCVYLMKQAIAKGVIFIIEKMASLNNSVNNAG
                     NALGKAFIDGANIAIRGVNKLIDLINKIPGINIGKVGEATFTPVKADNSYIKQQIDSL
                     NRWVGDAPEKVKLERMGYKDIGAAFQKGNALGTKWQNAITDKFKDTFDINKMLEDAKK
                     KLGLDDLWNKQNPLNNLGGFGGDLGKNVKDTAGNTAKMAKTMDKSQEDLKYLRDIAEQ
                     ETINRFTGVNIKIDMNNTNNISKDTDVDGIVNVLTEKLNDAMVVSAEGIV"
     gene            7119..7808
                     /locus_tag="KR440_001359"
     CDS             7119..7808
                     /locus_tag="KR440_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBH0698416.1"
                     /translation="MAYDFYLDGVQLPIAPPKLEVKVTNKNKTVDLINTGEVNILKKE
                     GLSEISFEAEFTHNKLPFCRGQFRDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNIKVSLEEYAISEDADNGSDTKVAIKLKQYRDYSTKKLVLAPPKNETGRPNVKIEPK
                     RVDSVNATNTKTYTVKAGDSLWSICQKQLGNGSLYKKVYELNKSMMDKANKGKNLSKY
                     TIYKGQVLKLG"
     gene            7801..9693
                     /locus_tag="KR440_001360"
     CDS             7801..9693
                     /locus_tag="KR440_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBH0698417.1"
                     /translation="MVDELVLANDRDVRLVIAHWEDFYEPAVIDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGIGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKILAKDFNLKYNVIEDTKYKISRVEENKTLFDMILTALDDTLRE
                     KKEMYTLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     SGKRDVYIAQDSAHMRSWGILQMFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     ALGDIRVRAGCLVPVFLNLGDIELQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKDEKKNGPAQSIMKKNTGKKVPAIFTAYYPGNNAMEGGKTDCNGKPL
                     DVKSRTVAGPMNREGVKKTWYTDDFLNKHPVFEYGDKVKIILPGTAYDNKVYTVKDNG
                     GRIYVETNGTYHIDILLANASECKKFGRKNGYIIIGGDEEQTYQVEGNNQSNTNNNSK
                     EDKLISIAKSKLGCNYVYGAEGPNNFDCSGFTQWCYKQIGIKIPRTASAQSKAGKAVD
                     LKDRSKWKAGDLLCRIGGGSSNHVVMYIGNNQIIHSPQTGDVVKIESVNSYRKGKAYT
                     HVRRFI"
     gene            9707..9967
                     /locus_tag="KR440_001361"
     CDS             9707..9967
                     /locus_tag="KR440_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HBH0698418.1"
                     /translation="MSQDLLQIIKKAAMDAVETSNPMRVVFGTIESISPLRVKIEQKL
                     SIGEIFLIQTDTFKRYTDKKMGDKLVLIRMQGGQQYLILDRM"
     gene            9972..10391
                     /locus_tag="KR440_001362"
     CDS             9972..10391
                     /locus_tag="KR440_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBH0698419.1"
                     /translation="MLPSDNLDYDIEDVSIINFDVRQEPSKTFKLHIEKSKIDGICDD
                     VEALKQTIFLILNTERYEHLIYSRNYGVELNDLIGEPISYVIPELERRITEALIQDDR
                     IENIDNFEFQNIKGKVQCRFSVHTKYGNIKAEKVVSV"
     gene            10392..11441
                     /locus_tag="KR440_001363"
     CDS             10392..11441
                     /locus_tag="KR440_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBH0698420.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLEATHTIAKGVFNIDIPLDSRFNLGE
                     YNYVAIERISEKTYKMKCETTGPVFELGKLIPIEYIDGLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNIQNYRDEVNKIQDVGGVKVYPTWDGGGTVKLVIINSNFKV
                     PSEELVNLVQEEIDPIGHQGEGLGLAPIGHRVTVEGVTSTTINISAEITYKNGYTWEN
                     IKSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDIANTMINDVKSNL
                     TIDSNSIVVRGEVVG"
     gene            11434..12051
                     /locus_tag="KR440_001364"
     CDS             11434..12051
                     /locus_tag="KR440_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HBH0698421.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVENKEIKTLHDKLKDLSN
                     DQFLEDLTISGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDNV
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSNIKPCNMTLDYTRVLISKDNY
                     MNFGITTLMGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEITIF"
     gene            12063..13088
                     /locus_tag="KR440_001365"
     CDS             12063..13088
                     /locus_tag="KR440_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBH0698422.1"
                     /translation="MAIDKSYYTIITDVGKAKIANASVTGNKVGFVKIQLGDGGGSEY
                     TPTESQTELKNVVWEGNIGNTTTDETAPNCIILESLIPSSVGGFMIREIGYLDDENNL
                     IAISKYKECYKPSIEQGAVVDMKVKTVLIVSNINNIELKIDPTIIFATLKDIQDLDAK
                     ISNVNTKIDTTKTELTSNIETAKTELNTRIDTENEKQNIKIDQLIAGGSNVASTQTIT
                     IDDWIDNQEGGFKATVTHGLLTQRITVSIIDATTKDNVVPDFTIIDDNSIEVRSGVKV
                     ELNVYVINGNAETHFINATVDDNRVSEMTTYSSKKIHEEIGKVAEQLTGINSNIISTV
                     NNDILPI"
     gene            13105..14631
                     /locus_tag="KR440_001366"
     CDS             13105..14631
                     /locus_tag="KR440_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698423.1"
                     /translation="MALSMSYFNLPDKRKYTKNLAFNPFAGGRQNFEWTGGDHGLNGE
                     FKETCLSCTYNGSTLNWGSGNVWVLGEYGQYTFTYNCESMHVDTQQKFPYTSNRIITI
                     KGRPVISGSDTSLGNKRKGFSVDFTVSDDTPNVNLIVRAYLDDKLIQNITPVVQNSTL
                     TATVTDSQLNSLSVDGNHKLKIQLNDGYDNFDRIFTFKKIEKGIDISTSLVTDSQAKF
                     TVTKIYSELTKIECYLDETLKETFTTDLYSEKTINYELIDNAIHTLKIVVTDAENVVE
                     EKVISISKNIMPLQPDATLQDISTKLTEIGQGVRNGKTSIINTLALKNIDASLNNTLV
                     ELSEKIKGGFDSGDASLQDLMNQLTQANNTISQLNTKYKVASGTVTSFADSTKIAYPY
                     LTDNVTKPGSWIKVSNLGFKPNIFFADFDYYDAEYKNNYKLFLFACNGVATQRGVDFS
                     SVTSFIRKSGDEYFHANGWLYSNSEGDVYFNNTGVQIPAYNFDSTQKHTYKWYAIKFI
                     "
     gene            14646..14939
                     /locus_tag="KR440_001367"
     CDS             14646..14939
                     /locus_tag="KR440_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698424.1"
                     /translation="MNVPNRIIYDQTGRTIFETGESCGDVLPHYTITELHYIDIEYGS
                     IDYTRNRVIGINIETKEPILEEIPVYITDEEKRIQELENQLLIAENEKVGGLL"
     gene            14939..15121
                     /locus_tag="KR440_001368"
     CDS             14939..15121
                     /locus_tag="KR440_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698425.1"
                     /translation="MNINNVVVRILAERILSKGLNPLKNREFQLDDVTNTEYRKAVED
                     YIIKNSGVVEGAEPTI"
     gene            15155..15721
                     /locus_tag="KR440_001369"
     CDS             15155..15721
                     /locus_tag="KR440_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698426.1"
                     /translation="MTFKELVNKVRNLVLEAKNVTIEDTESKFTSENVEGALKECIDR
                     ADEAFQEADSGKTLLSTAIGSPTTSEQTFQDYANYITGFKSNISNLETQLKSKYSIRY
                     GPIDGYDGNPFSANFGKSASYLIVYVYFRRSVYYYNPSGSSLGSNTGGSERAWITINS
                     NKTGFSVHSYDTSYESYPFTGYYIACFA"
     gene            15752..16042
                     /locus_tag="KR440_001370"
     CDS             15752..16042
                     /locus_tag="KR440_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698427.1"
                     /translation="MEEISINLLCAVAGVVISYLAFRNNSNRKIQDETETTTKLEQQI
                     TFLCENVRDIKHDVAKFNTSFLDISERVAKIEASTKQAHLRIDEIVNRIGGK"
     gene            16046..16303
                     /locus_tag="KR440_001371"
     CDS             16046..16303
                     /locus_tag="KR440_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBH0698428.1"
                     /translation="MDNLISFIPEQLLILVAALSIIGKGCKKYKQLDNKYIPVVLLIL
                     GIGFSIWMLGLNPVAVLQGVICWGVAIGINQTYKQLKDGEK"
     gene            16564..17427
                     /locus_tag="KR440_001372"
     CDS             16564..17427
                     /locus_tag="KR440_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HBH0698429.1"
                     /translation="MVEVKEEKTFDEQIDILKSRGLIINDKEDAKFVLSNVNYYRFTA
                     YLLSFKNDDGSYKEGTTFEEVYDIYRFNKEFRILLTDLLGSIEIAFRTYIAYTLAIKH
                     GACGYLERESFKDEKFYINFLTALEREKSNNSDKLFIIHHKEKYEGKLPIWVATEIMT
                     FGMLSKLYSNMLPEDTRYIKNNLCRVNTLLVKSWLQSLTQVRNQCAHYGRIYNNNFRI
                     ITIKNEYKKYNLDNKKIFSYILAMKHLTMDKLIWNSFFIKLQKLINDYNNSIDLKLIG
                     FPNNWIEILAK"
     gene            17506..18318
                     /locus_tag="KR440_001373"
     CDS             17506..18318
                     /locus_tag="KR440_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH0698430.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKE
                     GHKADVIICPEKQFKTKAEEKTYKIPRVNSGGYDLLIELHLNASDGQGKGSEVLYYSN
                     KGLEYATRICNKLGTVFRNRRAKLDKGLYILNSSNPTAVLIESFFCDNKEDYEKAKKL
                     GHEGIAKLIVEGVLNKNINNEGVKQMYKHTIVYDGEVDKISATVVGWGYNDGKILICD
                     IKDYVPGQTQNLYVIGGAACEKIGSMTKEKFTMIKGNDRFDTLYKALDFINR"
     gene            18412..>19217
                     /locus_tag="KR440_001374"
     CDS             18412..>19217
                     /locus_tag="KR440_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBH0698431.1"
                     /translation="MIFKKYIKSLLIVVSVTLIMNKSILFVNALDLESNNNIKSYKRK
                     NVKEIDISNKFVDESFRRDIVEKVLDNRDDPLTNEVASKDNYKIYESDLNKIESCLEL
                     DLHKNPDVSNPNKIDNLDGIENFRNLEKLDCSSNNLESVDLSKNIKLKYLDISGNKIL
                     NIDLKNNNLINELHCDANKLSNLDLKNNKNLVSLFFTSNELTEIDLSNNVNLENLQFL
                     NNNLKSIDLSHNKKLKSLNCTNNKLKSLDLSNNVFLKNLSCSGNNLSKLN"
BASE COUNT         7778 a         2076 c         3494 g         5869 t
ORIGIN      
        1 ttataaagca attaatttat aaaatatata taaataagta ggtatttatt tacttgaatt
       61 tcattgttta tataaaaaaa atggaaaaat atgtaataat tataggttat aataattgta
      121 gcaaggataa taatcgaaag tgcgaagggt gattattttc atattaaacg ccaaattcca
      181 aataaggaag gaggtgaaat tatatgatag gttttttatt aagcatacta gctggtgtta
      241 tatcagctta tatttatgac aaaataaaaa atcacccaga cgccaataag ggtgatttaa
      301 aaaaataata ttttcactta acaactgaaa ataatcactc tttgtaggaa taaattattt
      361 ccttgctttt attataccac aaattggtac agatattcaa aaataatatt tttatgatat
      421 aataaaaatg tagagatttt gcagtgagcg atatttgtga taaattgaag tttaacagtt
      481 gcaatataag gcgttgaggg tgtgtgataa atgttatcaa ttgcactact catggttcac
      541 tgcaaattta agagagttgt atatgtgtag gtattgaaaa tactcaactt attttggggt
      601 tttagattaa ctatatggaa tgtaaatgtt gtaagaagta tcattctatt ttttaatctt
      661 tctgttttag attaactata tggaatgtaa atttcagtga gaataagctt tattgtcgat
      721 gtaacactcg ttttagatta actatatgga atgtaaatag tacatataat gagtctttaa
      781 catcagttat gaaaggtttt agattaacta tatggaatgt aaatgattgt actttagcgt
      841 ctgcactagc tttgtctatc gttttagatt aactatatgg aatgtaaatt attttacaga
      901 tgaacaatta cagttacttc ttgaatgttt tatattaact atgtggattc aaaattaaat
      961 agaaaaaaga aagcacttac ttaaatagta ggtgcttttg ttttgctcaa attacttaat
     1021 tataaagttg ctaagtacaa ctttttagaa ttatacaata taaaaatgca ctaaagtgat
     1081 tgattttgct caagttggtc ggttgagtaa aataattaga aaaaaatgtt aaaaaactct
     1141 tgaaaagtgt cgcgatacaa tgtataatta tattatcgcg atacagaaaa gaggtgaaaa
     1201 ttattactga tagcagtaga gcagattact tcaagcagag acgacagaat aaaaaaactt
     1261 ttagtgttct actagataga gagaaagtag agaaaattga agaacattta aaaaagcaga
     1321 acaagactaa aactatttgg cttgaagaaa agattaatga agagctagaa aaagaggaat
     1381 aaaaaataag agacgttctc cccgaccaaa gattgaacat cccttattga cgtatattat
     1441 atacactaac tatagtatac gtcattcctt gaaaaaattc aattaaggag tgtaatagtt
     1501 atgaaaaatt taatagtaaa agagttcaat ggaagtcaaa tttatacttt tatgtggaaa
     1561 gaaaaatctt gttggatagc taatcaaata gttggattat tcgattatgc tgatgtatct
     1621 aaaacaatac aggattgcat aaaagcagaa gactttgaga ttgaacaaga gtatgatgta
     1681 ttgaaaggaa atgaatttaa tgattttgta actactttaa atgtagtcgc aaataatata
     1741 attagtaata aagctagaag tataactatt ttttatgaag atggtttata tggattttta
     1801 caatacacag acaaacctat tggtgtacag tttaggaaat ggcttagacg agaagtttta
     1861 ccaagcataa gacaaactgg tgcatacata actaacaatg ctaatcccga aaaactaaga
     1921 gaaaaagcaa gtgagattga gaaattacag ttagcttaca atagtacatc tatgttaaaa
     1981 gaactattag atggtgcagg ctttgacaat aaatccaaac tattaacagc taaaacatta
     2041 tataagaaag caggaattga tttaccaata gagataaacg aagaagaaca ttattttgat
     2101 acaaagcaaa tagcatctaa actgaaaata tattctaaga gtaataaacc agcacagttg
     2161 gctgtttgtg aaattattaa aaagattgat ttagaagaaa acgaagtcaa aggcgtttgg
     2221 gaaactaatg gttcttggac tggtactgta aataaatata caaagagtgt aatagataag
     2281 gttagaaatt ggatagagga aaataataga cctactaaga ttgcaggtga aaagaagaat
     2341 tatcatgtgg tttataagat tgagtaaatt tataagattg tattaaataa tttagtttag
     2401 ttttgggggg attaatacaa tgcatgagaa tttacttgat agtatagata ttgaaaagag
     2461 aaaagaagaa tttagaatta aacttttgaa aataagagaa acagatatag atatatataa
     2521 taagatagaa agtatagtat ataaactttc ttagaaaaaa ttagagaaaa ataattaaat
     2581 aaatagataa agcacttgga tatttcactg tttcaagtgc tttatatggt aaaaaatggt
     2641 ataataaaat ataggaattt tgcagtgttc gatttttcta ctcaagtata gtttaacaat
     2701 tggaatacaa ggcattgaga aggtttgata agtgttatca attgcactat tacccgctca
     2761 ctgcaaattt gagaaagtta tatgtgtgta agtcttggaa atacacaatt tattttgggg
     2821 ttttatatta actatatgga atgtaaatct ttgtaatggt agtgtattta agattgaaac
     2881 atcaagtttt atattaacta tatggaatgt aaatcaacat tagtagttgt ctttatacac
     2941 atagcatcac gttttatatt aactatatgg aatgtaaata cagctcccaa gacatacaac
     3001 gaatctgtaa catcagtgtt ttatattaac tatgtggtat gtaaaagact tatttacagc
     3061 tttatttgct aaatcagaag gttttatatt aactatgtgg acttaaaatt aaaaataatt
     3121 aaaaaacact tactaatgta ggtgtttttt tattgaaagg aagtgattat aatgtaaaaa
     3181 ttttgaagat atagtataat attcttataa aatataattt agggggatat tatgagagaa
     3241 ggaaagagaa gaagaggatg tctattttgg tttattctta ttattttatt ttctggagtt
     3301 gttggagcaa tagcagggaa cagtactaat aatgaaagca ctgaaaaaca gaaagaggat
     3361 ttaactaaat atattggtga agaaggtagt ataggagatt taaaattaac tgttaatagc
     3421 atttcaaaag ctagtgaaat atcagtagca tctggttatt tagcatacac tccagatagt
     3481 ggcaaatatg gtattataaa tttaacaata aaaaatcaaa ctaaggaaag ccaatctttt
     3541 atgctaaatt cattcacatt aataggacca gatgattcaa aatatgttcc atctttattg
     3601 attgatgtag gaaataaata tataactatg gatactgtaa atcctaattt agatgtaaca
     3661 ggtaatattg catttgaaat tcctaaggat ttactagttt ctgattgtaa actaaaatat
     3721 agtggttcag agcaagaatt tatattaaaa gaaaaataat atatagattt aaaaatatga
     3781 agcatttact ttaaataagt aagtgctttt ttaattaaaa ggaggtgata ataatgtaaa
     3841 aattttactt atatagtata attacattat aaaattattg tactgggggg aatcttatgg
     3901 ggttatttgg aaagaaaaaa gcagatatat gttgtatttg taatactgag ataggagtac
     3961 tgaatattga agatggttgg atatgcaatt cttgttttaa agaatattgt gatgcacttt
     4021 ctatgactaa agcacctaaa attttaagaa aattagatat tgaaaagact atatcatcaa
     4081 ctaaaaaaaa taatgaactt caaaaaatat ttaatacaac taataatata gaaaactata
     4141 tagaatttga tgaagataat aaaaaatggc ttgtgtctaa aaaaagtata aacgataaaa
     4201 aaactcctat cattcattct tatgaagata tcgtgaaatt tgaacttctt gaaaatggtg
     4261 aaactgtaac taaaggagga ataggaagag ctttagcggg aggaatttta tttggagaag
     4321 caggagctat agttggaggg ataacaggta aaaaaacaac aagaaaagtt gttgatacat
     4381 ttaaaattaa aataacaatt aataatattg ataatcctat tgagtatatc gaattaatta
     4441 ataaaaaaac aaaaactaat tctagtgctt atgagaaagc ttacaaagat gcccataaaa
     4501 ttttatcaac attatctgct attacacaaa gcattaaaga gacagacaat ataaatacta
     4561 aatctgtagc agatgagata ttaaaatata agaatctttt ggatatggaa gctattacac
     4621 aagatgaatt taatactaag aaaaaagaat tattgaattt gtaatataat aaacacttac
     4681 taatgtaggt gtttttttat atggaaattt atgaaaggag agtgagaaag tggcaacgat
     4741 acaaacttca ataaagattt tcgacggaat gacacctgct tttaggaata tgactacatc
     4801 tattaatacg acaattaata gccttgaaag gttacaacaa agattgcata atccgcttaa
     4861 cacaggtggg atacaagcat ctcaacaaag tttaaacaat attgaaaaca tattaaccag
     4921 gatagaacag aaaattggaa gaaatacaaa tgaacaagaa aactttaata ataagataaa
     4981 gcaaggaagt gaagcaggtt ctctattagt atctaaactc aaaagtttgg ctggcatata
     5041 tattggaata agaggtatag aaagtattac aaaagcagca gatacaattg caagtacaaa
     5101 agcacgttta aatttaatga atgatggatt acagacaaca gaacaactta ataagatgat
     5161 ttatttatct gctcaaagtg caagagctag ttatgcagat acggcagcac aagtcgctaa
     5221 acttggaata ttagcaggag atgcttttgg aagttcggca gaggtgataa agtttacaga
     5281 acttatgaat aaagcttttg taattggagg aacatcagca aatgaagcta gtgcggctat
     5341 gtatcagctt actcaagcta tgggtgcagg gaaacttcaa ggtgatgaat ttcgctctat
     5401 aatggaaaat gcacctttat tagccactaa aatagctgat gcaatgggaa aaactaaaga
     5461 ccaattaaag gaattgtcaa gtagtggagc aataacagca gatgttataa gaaatgcact
     5521 atttaaagct tctgatgaga tagaaaagaa attcgcaagt atgccagtca cttttagtca
     5581 agcacttact atgatgaaaa atgacgccta catgattttt gggcaaaccc tcggaaagat
     5641 aagtggagca ttgcaaagcg ttagatttag tgaaattgtt gtatctataa gaaatgttat
     5701 gattgcaata tcttcaaaca tttacgatac attaaatatt ataaaaaata ttttaaatag
     5761 tgaattcttt tcaaacttag tgcaaggatt tacattttgt gcggtagtaa taactaaaag
     5821 cttaggttct attgtaaata ctgcattaaa tatagttaat atatttgcac aaaattggag
     5881 tataataaaa cctattgtat ttggtgtagc ttctgttttt atattgttta gaggggtttt
     5941 gctagcgact aaaatagcta caataggtaa cgtaattgcg aatgttgctc acgctgcttc
     6001 ctcttcacta tcagcactta tgactaatat acaagcagca gcactgatgc gttcgaatgg
     6061 agctacttta tcagcaacaa tagccacttg gggtttaaat gcagctttat tagcttgtcc
     6121 aatcacatgg gtagtattag gtattctagc atttgtagta gtagtttttg ttgcagtagc
     6181 agcagtaaac aaattcgcag gtacaagttt aactgcctta ggagtaattg tgggtgctgt
     6241 atttgcagca gtggcagcaa tacaaaatgt tatgatttgg ttattaaatg gctgtatagc
     6301 tgtaaatgaa ggcattacaa atggttggaa tcaatgtgta tatctaatga aacaagctat
     6361 tgcaaaaggt gtaatcttta taatcgagaa aatggcatct ctaaataatt ctgtaaataa
     6421 tgctggaaat gcacttggga aagctttcat agatggggca aatatcgcaa tacgaggtgt
     6481 aaacaaatta attgacctaa taaataaaat accagggata aatattggta aagtaggaga
     6541 ggcaacgttt acgccagtta aggcagataa tagttacatc aaacaacaga ttgatagctt
     6601 aaacagatgg gtaggagacg caccagagaa agtaaaattg gagcgaatgg gatacaaaga
     6661 tattggagca gcatttcaaa aaggaaatgc acttggaact aaatggcaaa atgctataac
     6721 tgataaattt aaagatactt ttgacattaa taagatgcta gaagatgcaa agaaaaaatt
     6781 aggattagac gatttgtgga ataaacaaaa tcctttaaac aaccttggtg gatttggtgg
     6841 agatttagga aaaaatgtaa aggacacggc aggcaatact gcaaagatgg caaagactat
     6901 ggataaaagt caagaggact taaaatactt aagagatata gcagagcaag aaacaatcaa
     6961 tcgattcaca ggagtaaaca ttaaaataga tatgaacaat acaaacaaca taagtaaaga
     7021 tacagatgtt gatggaatag ttaatgtctt aactgaaaaa ctgaacgatg ctatggttgt
     7081 atcagcggaa ggaatagttt aggaaggaga gtgagaaaat ggcttatgat ttttacctag
     7141 atggagtaca actaccaatc gcaccaccca agcttgaagt caaagtgaca aacaagaaca
     7201 agacagttga tttgataaat actggagaag taaacatatt aaaaaaagaa ggattatctg
     7261 aaataagttt tgaagcagaa tttacacata ataaactacc tttttgtaga ggtcaattta
     7321 gagatgttca attcttttta agtaagctag aattactaaa aactgattgt aagccatttc
     7381 aatttattgt atctcgtgag ttaggtaaca aggtcttatt taacactaat ataaaagtat
     7441 ctcttgaaga gtatgctatt tcagaagatg cagataatgg ctcagataca aaagttgcaa
     7501 taaaattaaa gcaatataga gattactcaa ctaaaaagtt agttcttgcc cctcctaaaa
     7561 atgagactgg tagacctaat gtaaagatag agccaaaacg agttgattca gtcaatgcca
     7621 caaacactaa aacatataca gtaaaagcag gggatagcct ttggtcaatt tgtcagaaac
     7681 aacttggtaa tggttcatta tataagaaag tatacgaact aaataaatct atgatggata
     7741 aggcaaataa gggcaaaaac ttaagtaaat acactattta taaagggcag gtgttaaaac
     7801 ttggttgatg aattagtgtt agcaaatgat agagatgtaa gattagtaat agctcattgg
     7861 gaagatttct acgaacctgc tgtcattgat ggtatcacat gggagataga aagacgaggt
     7921 acaccttcta agttagagtt tacaatagtc atggatgata tactagagtt ttgcgaagga
     7981 aattctgtaa ggctgtatta taaaggaata ggcatatttt atggatatat atttcaaaag
     8041 aaaagagata aagaaaatca cattaaaatt gttgcttacg accagttgag atattttaag
     8101 aataaagata cttatgtata tagtaataaa actgcaagtg aacttgtaaa aatcttggct
     8161 aaagatttta atttaaaata caatgtcata gaagatacta agtataaaat atctagagtc
     8221 gaagaaaata aaacactctt tgacatgata ctaacagcac tagatgatac tttaagagag
     8281 aaaaaagaaa tgtatacctt gtatgatgat tttggaagaa taacattaaa gaatgttgca
     8341 tcaatgaaac tagatactgt catgaacaat gatgtaattg aggattttga ctataattca
     8401 tcaatagata gtgatactta cacaaagatt aaacttgtaa gagacaacga ggagtcagga
     8461 aaaagggatg tatatattgc tcaagactca gctcacatga gaagttgggg aatacttcaa
     8521 atgtttgata cagtagacaa aaacatgagt gaagcagaga taaaacaaaa gtgtgatata
     8581 cttctaaaac tatataataa gaaaactaag tcattaagtt taaaaaatgc acttggagat
     8641 attagagtga gagcaggttg tttagtacct gtttttttaa atctaggaga tattgaattg
     8701 caaaattata tgttagttga gaaagtaaaa catacatttg aaaataattc acatttcatg
     8761 gatttgacct tggttgatgg agacgaattt gcttcatatt cttcaagctc atatagtagt
     8821 ggaaatacta ataataaaga tgaaaagaaa aatggtcctg cacaaagtat tatgaaaaaa
     8881 aatacaggta aaaaagttcc tgctatattt actgcatatt atccaggaaa caatgcaatg
     8941 gaaggtggaa aaacagattg caatggaaag ccacttgatg taaaatcaag aactgttgct
     9001 ggtccaatga atcgagaagg agttaagaaa acttggtata ctgatgattt tctaaataaa
     9061 catccagttt ttgaatatgg agataaagta aaaattatac ttcctggtac tgcctatgac
     9121 aacaaagtat atacagttaa agataatgga ggaagaatat atgttgaaac aaacggaaca
     9181 tatcatatag atatactatt agctaatgct agtgaatgta aaaaatttgg tagaaagaat
     9241 ggctatataa ttataggtgg agatgaagaa caaacatatc aagttgaagg taataaccaa
     9301 agtaatacaa ataacaattc taaagaagat aaattaatta gtatagcaaa aagtaaactg
     9361 ggttgtaatt atgtgtatgg agcagaaggt cctaataatt ttgattgcag tgggtttact
     9421 caatggtgtt ataaacaaat aggtataaaa attcctcgta ctgcttctgc acaaagtaaa
     9481 gcaggaaaag cagtagattt aaaagataga agcaagtgga aagcaggaga cttattatgt
     9541 agaattggtg gaggaagtag taatcatgtt gtaatgtaca ttggaaacaa tcaaataatt
     9601 cattcaccac aaacaggaga tgtggtaaaa atagagtctg ttaattctta tagaaaagga
     9661 aaagcataca cacatgtgag aagatttata taagtgaggt ggcaatatga gccaagattt
     9721 attacagata ataaaaaaag ctgcaatgga tgcagtagaa acaagcaacc caatgagggt
     9781 tgtatttgga acaatagaaa gtattagtcc tctaagagtt aagatagaac aaaaactatc
     9841 tattggtgaa atttttctaa tacaaacaga tacatttaaa agatatacag ataaaaaaat
     9901 gggagataaa ttagtcttaa ttcgtatgca aggagggcaa caatacttga ttttagatag
     9961 gatgtgataa agtgttacca agtgataatt tagattatga tattgaagat gtatcaataa
    10021 ttaattttga tgtaagacaa gagccaagta agacatttaa acttcatata gaaaagtcta
    10081 agattgatgg tatttgtgat gatgtagaag cattaaaaca aaccattttt ttaattttaa
    10141 atactgaaag gtatgagcat cttatttatt ctagaaatta tggtgttgaa ttaaatgatt
    10201 taattggaga acctatttca tatgtaatac ccgaacttga aagaaggata acagaagcac
    10261 taattcaaga tgataggatt gaaaatatag ataattttga gtttcaaaat ataaagggta
    10321 aagtacaatg tagattttca gttcatacaa aatatggaaa tataaaagca gagaaggtgg
    10381 tgagtgtata attgtttgag ttaatgacat ttgaaaatat aattaaaaga atgttagata
    10441 gtgtaccaga tacttttgat aaaagggaag gttctataat atataatgct cttgctcctg
    10501 ttgctataga acttacagaa acatacattg ctatggatga attactagac caaacattcg
    10561 tagatactgc tagttattat tatttagaga agagatgtaa agaaagggga ataacaccac
    10621 ttgaagccac tcatacgatt gctaaaggag tttttaacat agatattcca cttgattcta
    10681 ggtttaatct aggagaatat aactatgtag caattgagag aatatctgaa aaaacatata
    10741 aaatgaaatg tgaaactact ggacctgtat ttgagttagg aaaactaata cctattgaat
    10801 atatagatgg tcttgaaact gctgaactaa ctgaaatctt gataaatgga gaggatgaag
    10861 agtcagaaga tagtttaaga caaagatatt atgatagcct aaattcacag agttttggcg
    10921 ggaatataca aaactatagg gatgaagtta acaaaataca agatgttgga ggagttaaag
    10981 tttatccaac ttgggatgga ggtgggactg ttaagttagt aataattaac tctaatttta
    11041 aagtgccaag tgaagaatta gtaaacttag ttcaagaaga aattgaccca attggacacc
    11101 aaggagaagg cttaggatta gcaccgattg gacaccgagt cacagttgaa ggagttacaa
    11161 gtacaactat aaacatatca gcagaaataa catacaaaaa tggctacact tgggagaata
    11221 taaaatcaat tgcagaagaa gcaatagacg actatttaaa tgaacttaac atgagttggg
    11281 aagatgaaga aaacttaata gtccgtatat ctcaaattga aactagatta cttagtattg
    11341 atggagtatt agacattgca aatacaatga taaatgatgt taaatctaat ctaacaatag
    11401 atagtaacag catagtagtg agaggtgagg tagttggata aagagattaa tctaataaat
    11461 tacttgccac aaattctaca agataaagaa gaatatataa aagtatttaa tgtagaaaat
    11521 aaagaaataa aaacactaca tgataaatta aaggacctat caaatgacca gtttttagag
    11581 gacctaacta taagtggtat aaaaagatgg gaaaagataa tgtctataac tcctaaaagt
    11641 aatgagagtt tagaagatag aaggtttagg atttttagta aatatataag taaattacct
    11701 tactcagaga gatttttaag gaactggcta gataatgtag ttggagaagg caattatgaa
    11761 ttaactatta ataatgctac ttataacata caccttgaga gtgatgctag aaatcaagat
    11821 tggtttgagg aagttcattc ttttgtaagt aatattaagc catgtaatat gactttagat
    11881 tacactagag tgcttataag caaagacaat tatatgaatt ttggtataac aaccctaatg
    11941 ggtcaagaaa taactatata cccttggagt ccaccagata tagaaactta tggagaaatt
    12001 gatgtattaa ctggcaatgg agttggatac caagagataa caatatttta ggaggtgata
    12061 tattggctat agataaaagt tattacacta taattacaga tgtaggaaaa gcaaagatag
    12121 caaatgcaag tgtcacaggt aataaagtag ggtttgtaaa aattcaactt ggtgatggtg
    12181 gaggaagtga gtatactcct actgaaagtc agacagaact taaaaatgtg gtatgggaag
    12241 gcaacatcgg aaatacaacc acagatgaaa ctgcgccaaa ctgcataata ttagagagtt
    12301 taataccatc aagtgtaggt ggatttatga taagagaaat aggatattta gatgatgaaa
    12361 ataatctaat tgcaatctct aaatataaag aatgttataa accttctata gaacaaggag
    12421 cagttgttga tatgaaggtt aaaactgtgc ttattgtatc taatataaat aatatagaac
    12481 ttaaaattga ccctacaata atttttgcta cgctcaaaga tatacaggat ttagatgcta
    12541 aaataagcaa tgtaaacact aaaattgata caactaaaac agagttaaca agcaacatag
    12601 aaactgctaa gacagagtta aacactagaa ttgacacaga aaatgagaaa caaaatatta
    12661 aaattgacca gctaatagca ggtggttcaa atgttgcatc tactcaaaca ataacaattg
    12721 atgattggat agataatcaa gaaggtggat tcaaagcaac tgtaacacat ggtttattaa
    12781 cacagagaat aactgtaagt attatagatg ctactacaaa agataatgta gtacccgatt
    12841 tcacaattat agacgataat tctattgagg ttagaagtgg agtaaaagtt gagttaaatg
    12901 tttatgtgat taatgggaat gcagagactc attttattaa tgcgactgta gatgataata
    12961 gagtgtctga aatgactact tattcatcaa aaaagataca tgaagaaatt ggtaaggtag
    13021 cagagcaact gacaggaatt aatagtaata ttatatcgac tgtaaataac gacatactac
    13081 ctatatagaa aggagcgtga aaaaatggcg ttatcaatga gttattttaa tttacctgac
    13141 aaaagaaaat atactaaaaa tcttgcattt aatccatttg caggtggaag gcaaaatttt
    13201 gagtggactg gtggtgacca tggactaaat ggtgagttta aagaaacttg cttaagttgc
    13261 acttacaatg gttcaacatt aaattggggt tcaggaaatg tttgggtctt gggggaatat
    13321 ggtcaatata catttactta taattgtgaa tcaatgcatg tggatacaca acaaaaattt
    13381 ccgtatactt ctaatagaat aataactata aaaggtagac ctgtaatatc tggttcagat
    13441 acttcacttg ggaataaaag aaaaggattt agtgtagatt ttactgtaag tgatgatact
    13501 ccaaatgtta atttaatagt acgtgcttat cttgatgata aattaataca aaacattact
    13561 cctgttgtac aaaattcaac tttaacagca acagtaacag atagtcaact aaactcttta
    13621 tccgtagatg gaaatcacaa actaaaaata caattaaatg atggatatga taattttgat
    13681 agaattttta cttttaaaaa aatagaaaag ggaattgata tatcaacatc attagtaact
    13741 gatagccaag ctaagtttac tgtaacaaaa atatacagtg aattgacaaa aatagagtgt
    13801 tatttagatg aaacattaaa agagactttt actacagact tgtattcaga aaagactata
    13861 aactatgaac ttatagacaa tgcaatacac acattaaaaa tagttgtaac tgatgcagaa
    13921 aatgttgtag aagaaaaagt tataagtata agtaagaata taatgccatt gcaacctgat
    13981 gcaacattac aagatatatc aactaagtta acagagattg ggcaaggcgt tagaaatggt
    14041 aaaacaagca ttataaatac tttagcatta aagaatatag atgcaagttt aaataataca
    14101 ctcgttgagt tatcagagaa aattaaggga ggttttgata gtggagacgc tagtttacaa
    14161 gatttgatga atcaattaac acaagctaat aatactatat cgcagttaaa tactaagtat
    14221 aaagttgcta gtggtacagt tacttctttt gcggatagta ctaaaattgc ttatccatat
    14281 ctaaccgaca atgttactaa acctggttct tggattaaag ttagtaattt aggctttaaa
    14341 cctaatattt tctttgctga ttttgattac tatgatgctg aatataaaaa taattataaa
    14401 cttttccttt tcgcttgtaa tggtgttgcc acacaaagag gtgttgattt ttcaagtgtt
    14461 acatctttta tcagaaaaag tggtgatgag tattttcatg ctaatggatg gctatatagt
    14521 aactcagagg gggatgtcta ttttaataat actggcgttc agattccagc ttataacttt
    14581 gattcaactc aaaaacatac ttataaatgg tatgctatta aatttattta aaaggaggaa
    14641 aaaacatgaa tgttccaaac cgaataatat atgaccaaac aggtagaaca atctttgaaa
    14701 caggcgaatc ctgtggagat gtattgccac attatacaat aacggaattg cattatatag
    14761 atattgaata tggaagtata gattatacaa ggaatagagt tataggtata aatatagaaa
    14821 caaaagaacc gattttagaa gaaataccag tatatataac agatgaggaa aagagaatac
    14881 aagagttaga aaatcaatta ttaattgcag aaaatgagaa agtaggagga ttattataat
    14941 gaatataaat aatgttgtag taagaatatt agcagaaaga atattaagta agggattaaa
    15001 tcctctaaaa aatagagaat ttcagttaga tgacgtgact aacacagagt acagaaaagc
    15061 tgtagaggat tatattataa aaaatagtgg agtagtagaa ggagcagaac caactatata
    15121 gagggttctt tttttattga aagaaggtga ctaaatgact tttaaagagt tggttaataa
    15181 agttagaaat cttgtattag aagcaaagaa tgtaactata gaagatacag aaagtaaatt
    15241 tacaagtgaa aatgtagaag gagcattgaa agaatgtata gatagagcag atgaggcttt
    15301 tcaagaagcc gatagtggaa aaacactttt atcaactgct atcggctctc ctactacatc
    15361 agaacaaaca tttcaagatt atgcgaacta tattacagga tttaagagca atataagcaa
    15421 tttagaaact caattgaaaa gcaaatattc tattagatat ggtcctattg atggatatga
    15481 tggtaatcct ttttctgcta attttggcaa gagtgcaagt taccttattg tctatgttta
    15541 ctttagaaga agtgtatatt attataatcc cagtggtagt tctttaggaa gtaatacagg
    15601 aggttctgaa cgtgcatgga ttactataaa cagcaataaa actggttttt cagttcattc
    15661 atatgatact agttatgagt catatccttt tacaggttat tatattgctt gtttcgcata
    15721 ataaattata ttattaaaac taggaggatg tatggaagaa attagcataa atctactatg
    15781 tgcagtagca ggagttgtaa tatcctactt agcatttaga aataactcaa acagaaagat
    15841 acaagatgaa acagaaacaa ctacaaaatt agaacaacaa ataacttttt tatgtgagaa
    15901 tgtaagagat ataaagcatg atgtagcaaa gtttaatact agtttcttag atattagtga
    15961 acgagttgca aaaatagaag caagtacaaa acaagcacat cttagaattg atgaaattgt
    16021 aaatagaatt ggaggaaaat aagagatgga taatttaata agttttatac cagagcagtt
    16081 gctaatttta gtagctgctc tctctattat aggtaaaggt tgtaaaaaat ataaacaatt
    16141 agataataaa tacattccag tagtgttact gatacttgga ataggtttct caatatggat
    16201 gctaggatta aatcctgttg cagtcttaca aggtgtaatt tgttggggag ttgcaatagg
    16261 tataaatcaa acttacaaac agttgaagga tggtgaaaag taatgaaatt aacaaaaata
    16321 ttattactca caaaaattta aaaaatttca ttaaatagag tatactttta tagctaataa
    16381 gtatataata atagtacaga actcgtaaag catttatatg ctcaaatata cgggacattg
    16441 atttttgaag gaacccgcca agcttctttt atagctcaaa taggcgggac agataaattt
    16501 acccactaat attaattggt gggttttatt atataaaagt atatctatga aaaggtgaaa
    16561 acaatggttg aagtaaaaga agaaaaaaca tttgatgaac aaatagatat tttaaaaagt
    16621 agaggattaa taataaatga taaagaagat gctaaatttg tattaagtaa tgtcaattat
    16681 tataggttta cagcatatct tctaagtttt aagaatgatg atggctcata taaagaagga
    16741 actacgtttg aagaagttta tgatatatat aggtttaata aggaatttag gatattatta
    16801 acagatttgt tagggagcat agaaatagca tttaggacat acattgcata tacattagca
    16861 attaaacatg gtgcttgtgg atatctagaa agggagagtt tcaaagatga aaaattctat
    16921 attaattttt tgacagcatt agagagagaa aaaagtaata attcagataa gctttttatt
    16981 atacaccata aagaaaaata tgaaggaaaa cttcccattt gggttgcaac ggaaataatg
    17041 acttttggta tgttgtcaaa attatattca aacatgttgc cagaagatac tagatacata
    17101 aaaaataatt tgtgtagagt gaatacttta ttagttaaat cttggttaca atcattaaca
    17161 caggtcagaa atcaatgcgc tcattatggt agaatatata ataataattt ccgtattata
    17221 acaataaaaa acgaatataa aaagtataac ttggataata aaaagatatt ttcttatata
    17281 cttgctatga agcatttgac tatggataaa ttaatttgga atagtttttt tataaaactt
    17341 caaaagttaa ttaatgatta taataattct atagacttaa agcttattgg ttttcctaat
    17401 aattggatag agatattggc taaataaaat agttacttta agaagtttat aaacacttac
    17461 tatatgtaag tgttttttta ttgaaaagaa ggaggaaaat aaataatgaa aatatgtatt
    17521 acagtaggac acagtatttt aaaaagtggt gcatgcactt ctgctgatgg agtagttaac
    17581 gaataccaat acaataaatc tcttgcacca gtattagcag atacatttag aaaagagggt
    17641 cataaggcag atgtaataat atgtcctgaa aagcagttta aaactaaagc agaagaaaag
    17701 acttataaaa tacctagagt taatagtgga ggatatgatt tacttataga actacatcta
    17761 aatgcaagtg atggtcaagg aaaaggttca gaagttctat attatagtaa taaaggttta
    17821 gagtatgcaa ctagaatatg taataagcta ggtacagtat ttagaaatag aagagctaaa
    17881 ttagataaag gattatatat cttaaatagt tcaaatccta cagcagtatt aattgaaagt
    17941 ttcttctgtg ataataaaga agattatgag aaagctaaga aactaggtca tgaaggtatt
    18001 gctaagttaa ttgtagaagg tgtattaaat aaaaatataa ataatgaggg agttaaacag
    18061 atgtacaaac atacaattgt ttatgatgga gaagttgaca aaatctctgc aactgtagtt
    18121 ggttggggtt ataatgatgg gaaaatactg atatgtgata taaaagatta cgtgccaggt
    18181 cagacgcaaa atctttatgt cattggtggt gcagcatgtg agaagattgg ttctatgact
    18241 aaagaaaaat ttactatgat aaagggtaat gatagatttg atacacttta taaggcatta
    18301 gattttatta atagatagat tatataaaat attttagtat ttcttgaaaa tcataagtat
    18361 ggtaatattt agataaatat tatttattat taataggtat ggggggagat tatgatattt
    18421 aagaagtata taaaatcatt actgatagtt gttagtgtta cattgataat gaacaaatca
    18481 atattatttg taaatgcatt agatttagag agtaacaata acataaaatc ttataaaaga
    18541 aagaatgtaa aggaaattga tataagcaat aaatttgttg atgaatcctt tagaagagat
    18601 attgttgaaa aggtattaga taatagggat gatccattaa caaatgaagt tgcatcaaaa
    18661 gataactata aaatatatga aagtgattta aataaaattg agagttgctt agagttagat
    18721 ttacataaaa atccagatgt ttctaatcca aacaaaatag ataatttaga tgggatagaa
    18781 aattttagaa atctagaaaa gcttgattgt agttcaaata atctagaaag tgtagattta
    18841 agtaaaaata taaaattaaa atatctagat attagtggaa ataaaatatt aaacattgat
    18901 ttaaaaaaca ataatcttat taatgaatta cattgtgatg caaataaatt atcaaattta
    18961 gaccttaaaa ataataaaaa tttggtaagt ttatttttta ctagtaatga attaacagag
    19021 atagatttaa gtaataatgt gaacttagaa aatttacaat ttcttaataa taatttaaaa
    19081 agtatagatt taagtcataa taaaaagttg aaatctctaa attgtactaa caataaactt
    19141 aaatcccttg atttaagtaa taatgttttt ttgaaaaatt taagttgttc aggtaataac
    19201 ctgagtaaat taaatat
//
LOCUS       DAEPOZ010000049        19105 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.049, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000049 DAEPOZ010000000
VERSION     DAEPOZ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19105
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.049"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            200..778
                     /locus_tag="KR440_001375"
     CDS             200..778
                     /locus_tag="KR440_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBH0698432.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            817..1395
                     /locus_tag="KR440_001376"
     CDS             817..1395
                     /locus_tag="KR440_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBH0698433.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLRHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            1481..2110
                     /locus_tag="KR440_001377"
     CDS             1481..2110
                     /locus_tag="KR440_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBH0698434.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            2100..4859
                     /locus_tag="KR440_001378"
     CDS             2100..4859
                     /locus_tag="KR440_001378"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBH0698435.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            4946..6508
                     /locus_tag="KR440_001379"
     CDS             4946..6508
                     /locus_tag="KR440_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HBH0698436.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGVIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDIHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            6509..7645
                     /locus_tag="KR440_001380"
     CDS             6509..7645
                     /locus_tag="KR440_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBH0698437.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            7709..8785
                     /locus_tag="KR440_001381"
     CDS             7709..8785
                     /locus_tag="KR440_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HBH0698438.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHSITGILIPKANSELIENCIEALYSMNLQDMYVI
                     PIIETREFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            8763..10001
                     /locus_tag="KR440_001382"
     CDS             8763..10001
                     /locus_tag="KR440_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HBH0698439.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGVRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELNCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKKHYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            10055..11143
                     /locus_tag="KR440_001383"
     CDS             10055..11143
                     /locus_tag="KR440_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HBH0698440.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNVSLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKHFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            11171..11962
                     /locus_tag="KR440_001384"
     CDS             11171..11962
                     /locus_tag="KR440_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HBH0698441.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYANIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKPDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDFSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFKGMSGTEEILSNILDENCLLNI"
     gene            complement(12077..12631)
                     /locus_tag="KR440_001385"
     CDS             complement(12077..12631)
                     /locus_tag="KR440_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBH0698442.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            12826..14028
                     /locus_tag="KR440_001386"
     CDS             12826..14028
                     /locus_tag="KR440_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HBH0698443.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            14085..15065
                     /locus_tag="KR440_001387"
     CDS             14085..15065
                     /locus_tag="KR440_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBH0698444.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLTEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            15312..16265
                     /locus_tag="KR440_001388"
     CDS             15312..16265
                     /locus_tag="KR440_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBH0698445.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RVPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAVFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            16255..17211
                     /locus_tag="KR440_001389"
     CDS             16255..17211
                     /locus_tag="KR440_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBH0698446.1"
                     /translation="MKLSLNQKIYFVSILLFISIALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKMLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            17208..17963
                     /locus_tag="KR440_001390"
     CDS             17208..17963
                     /locus_tag="KR440_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0698447.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRSLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            18055..19020
                     /locus_tag="KR440_001391"
     CDS             18055..19020
                     /locus_tag="KR440_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH0698448.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTIKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAENLVGLPKTSLPASLEKYKDKKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT         7550 a         1937 c         3419 g         6199 t
ORIGIN      
        1 acatagcttt tatacattta ataaagtatg taaaatctat gtaaaaagta agaaatataa
       61 acattttttt aattatattt taaaaatatc aagcaagttt tcttcacaaa tgaataaaaa
      121 atgttataat atgattaaag aaaaagataa ataattagca agatttgttt agctaaaatt
      181 aacaagggag cgataaatta tgggtataac attatcaaaa ggccaaaagg ttgatttaac
      241 taaaggaaat ccaggtctta aaaatatttt agtaggttta ggatgggata ctaacaagta
      301 tgatggtgga tttgactttg atttagatac agcagcattt ttaacaggag caagtggaag
      361 tgttactaat gatggagatt ttgtatttta caacaattta aaacatacat ctggagcagt
      421 tgagcatcta ggagataaca gaactggcga aggtgatgga gatgatgaac aaatagttgt
      481 tgatttatct aagattcctg gagaaataag caagatttct tttactgtta caattcatga
      541 tgcgacagaa agaagacaaa attttggaca agtaagtaat tcttatataa gaatagtaga
      601 taaagacaca aatgaagagt taataaagta tgaattagga gaagatttta gtatagaaac
      661 agctatagta gtagctgaaa tatataagca caatggagaa tggaagttta atgcactagg
      721 ttctggtttt gaaggtggtc ttgctgcatt atgtggaaac tttggaataa acttataatt
      781 tcaactataa caataaacta aagggggata ttttccatgg ctatagtatt aaaaaaagga
      841 caaaaaatcg atttaactaa gggaaatcca ggtcttaaaa atataaaact tggactagga
      901 tgggatacaa attcatttga tagtggctat gattatgatt tagatgtaag tgttttcatg
      961 gttggagaat ctcaaagagt tgaaaaagat gaagatttta tattctacaa taacttaaga
     1021 catccatctg gagcagttga acacttaggt gataatagaa ctggtgaagg tgatggagat
     1081 gatgaggaaa tattagtgaa tttagagaca atgccaaagc atatacaaaa gatagctgta
     1141 acagttacaa tatatgaggc tgctgaaaga agacaaaatt ttggacaagt aagcaactca
     1201 tatataagag tattaaattc tgaaaatgat gaagaaatat taagatatga cttaggtgaa
     1261 gaatttagta tagaaactgc aattacagta tgtgagatat ataagtataa tggagaatgg
     1321 aagtttagtg cagttggagc aggatttgaa ggtggcttag aagcactttg tagaaactat
     1381 ggcttaaatg tttaatttaa gcaatataat atattaaata taaataacct atacaaataa
     1441 tgtataggtt atattatata aaattttgtg gaggtttagt atgtcgatta atttatcaaa
     1501 aggtgataaa atagatttaa aaaaatctaa cccaggactg agtaacatac ttgtaggtct
     1561 aggatgggac cctgtgcaac aatctggagg aggttttttt aaatcgttat ttggtggagg
     1621 acaagctgat atagattgtg atgcatctgt atttatgctt aatcaagaag gtaaattaag
     1681 tggaataaaa gatttaattt attttggaaa tctaaaaagt gcctgtaaat cagttttgca
     1741 tacaggagac aatctaactg gtgagggtac aggtgatgat gaacaaatcc tagttaattt
     1801 ggataaagtt ccatcaaata ttcataaatt attgtttgta gtaaatatat acaattgtgt
     1861 agatagaaaa caacactttg gaatgataga aaatgcctat ataagagttg aagaccaagg
     1921 taataagaaa gagatagcaa aatacaatct atctgataat tattctgaaa aaactactct
     1981 tatagtagga gctatatata gaaaagatgg aagttggcaa tttaaagcaa ttggtgaagg
     2041 aacaaaagat gcgggtctta aagaagtaat gcaaaattta gacaggattg agtgtgcata
     2101 tggaatttaa taatgatatc aaatattata atgtagataa agaagaagtt acaaaagact
     2161 tatctgttaa tcctgaaaaa ggtttatcag aatcagaagt caagactaga agagaaaaat
     2221 atggtttgaa tgaatttact ccaaaagaag agggtagttt ttgggatgat ttaaaagaaa
     2281 gtttaagtga accaatgata gttatcttga ttattgcagc agtagtaagt gcagtaattg
     2341 gggaaactca tgatgcaata gggattgtag gtgctatagc aataggtata gcaataggta
     2401 tgattacaga aggtcgttct aagaaagcag cagaggctct ttctaagttg actgaaaaca
     2461 tagaagtaaa agtattgaga gatggggaag tacatcaaat ttctaagagt gaattggtac
     2521 caggtgatat agtatatatt gaaactggtg acatggtacc tgctgatggt agacttatag
     2581 aaagtataaa ccttaaaatt agagaagata tgttaacagg agaatctgat gacgtatcta
     2641 aaaagtgtga tgtagtagtt tctatggaag agatagaatc taaaggagca gtagtagtac
     2701 aggagccaat acctgcaaaa cagataaata tggtgtttgg tggtacactt gtagcttatg
     2761 gtagaggagc tctagttgta acttcaattg gagataatag tgaaatgggt aaaattgctc
     2821 aaaacttatc agaaacagat gagcaaactc cacttcagat aaaacttgga aatttaggtg
     2881 gtttgatagc caaagtttct agtgcagtag caggattact atttatattt atggttttcc
     2941 aaatgataag taagaatgta ttaaatgtag atatgtctgg aatattaccg tttttagaat
     3001 caatagaccc agttaaaaca gcatttacag tatgtgttgc gttgatagtt gcagcagtgc
     3061 cagaaggtct tccaactatg ataaatatga cacttgcaat aacaatgcaa aaaatggcta
     3121 aaataaatgc attggttacc aaaaaagaag cttgtgaaac gataggttct gtttctgtaa
     3181 tatgttcaga taaaactggt acattaactg aaaatagaat gactgtagaa gttgcatatg
     3241 ttgatggcag atatattgaa agttcagaag aggaaataaa tagttatttt gaagaaaact
     3301 gtatgataaa ctctacagct gatgtagagc acaatgatgg tgatattaag tatttaggaa
     3361 gtgctactga atgtgcattg cttttatatt acaaaaacgt agattataga caagttagaa
     3421 aaaattctaa tatagtagct caaaatcctt ttacttcaga ttcaaagaga atgacttctg
     3481 ttataggtca agataatcat catgtactat tatcaaaagg agcaccagaa gtcttacttg
     3541 aattatgttc acatattcaa agaggaaaag acatagttcc attaactgaa aatataaaac
     3601 atgagatttt agaagaaata aagaaattgc aaataaaatc aatgagaact ttagggtttg
     3661 catataaaga aatctcacaa gctgaagaag aagcagcagt aactgctgaa agtgatgcta
     3721 tgaatgtaag tgcaatggaa aataatttag tatttagtgg ttttgttggt ataagagacc
     3781 cacttagaaa agatgttata gagtctgtaa atacagcaaa taaagctggt gtttctgtaa
     3841 aaatgcttac aggagataat ataaatacgg caagagctat aggtgaagag ttaggcctat
     3901 taaaaaataa tatgagagtg gttgaagctt catatataga tactttagat gatgaagaat
     3961 taaaacaaga gatacaaagt atttcaatcg tggcaagaag taaaccagat agtaaaatga
     4021 gaatagtttc agctctgcaa aagagcgggg atgttgttgc agtaacaggt gatggtataa
     4081 atgatgctcc agcactttca aaagcagatg ttggtatagc aatgggtata tctggtacag
     4141 aggtatctaa aaacgcagca gacataattt taactgatga tagttttagt accattgtaa
     4201 aaggtattaa atggggtaga ggaatttatg ataacttcca aagattcgtt caattccaat
     4261 taactgtaaa tgtaattgca tttttagtgg ctataatatc tcaagttatg ggacaagaga
     4321 tgccatttac tacaatacaa ttattatggg taaacataat tatggatgga ccaccagcac
     4381 tagcattagg tttagagcca gttagagacc atgtgttaaa tagaaaacct gttgatagaa
     4441 aagccaatat aatttctaag tcaatggtgt atacaatagt attaaatgca ttttatataa
     4501 cagcaatact aatgttacaa tctacattta atttcttagg agttgatgaa gctcataagg
     4561 gaacagtaat gtttgcgtta tttgctttta gcgcactgtt taatgcattt aactgtagag
     4621 agtttaatgc agatagtatc ttccctaatt ttacacataa caaattagca ctacaaataa
     4681 tagtgttgac aggaattgca caagtaatat ttactcagct attccaagat ttctttaact
     4741 ctgtagcaat ggattttatg acatgggtaa aagttttatg tgtagcagca agtgtaattg
     4801 tggttaatga agttgtaaaa tgtataataa gactggtaaa accaaaaaag actgcttaaa
     4861 acaaattttt tagaagaaaa cagactgact caaaatatga attttgggtc agttctgaat
     4921 aaacatttaa attgggagga tagttatggg tgactcaaag gtatttgaaa aaatattctc
     4981 cagccaatca gatagaggaa attacactcc ttcaaaggga tatctaagtt attttatttc
     5041 gtatattggt ttagaagatg aagttttgta caatctcgag atatttaaga caaaacaaaa
     5101 tattgatagc aaaaaagata ttgctttatt tactgatgtt atagcaaatc ctagtgattt
     5161 tgatattata aattatttta aaagtggact tcaaaaatat agaactagca tggaagatgt
     5221 agatataaat attttgggat ttgaagagat tgattataaa ataaagcaag caatggatag
     5281 agtgttaaaa gaagaggaaa aagaatttac taatgataga gttaaacaaa attttatagt
     5341 gaaaataatg gcatggataa aaatttatat cggtgcttta gatataaata aaaatgaagc
     5401 tccaaaagta atattttatg gagatataaa aaaacatgaa gtttatttac tcttaatatt
     5461 atacttggca ggttttgatg ttttatatct taatccaaat tcaaagagta atatagatat
     5521 tttaaaatca gaaagataca atatagagtt tgaagaagcc aacataatag aagaaaaaat
     5581 ttcttttgaa gaaagggtta ttttagggga aaaaatagat aaaagttctg taaaaaaagc
     5641 ttttactgtt ggggcagaag catcaaagcg tataagtgag gaattgttaa atgatgcagg
     5701 atttataaaa ccttggcaac tccaagatag aaaaataaaa aatctacttc taagttcaac
     5761 tgttgatgaa atatcaatat actggaatca accacttaaa ttaagaccag gatttaagtt
     5821 taatgatgct attgtagaag ctccaaattt tctttctaag attaatggaa tatataatga
     5881 taagaatgaa tatattaagt ttttagattt attaagagat tcggaaagtt caacatttat
     5941 tgaatttaat ggagatgtag atagattttc aaaagctttt acgagagaag cttttagttt
     6001 atccttttta ttggattcaa aaggtgtaat agataaaaac tctgttttaa ataataaaga
     6061 ttattccata tcaacactag cacttaatca acaaattatg attttagaga aagtggaaga
     6121 gttgttagaa ggaagtatgt ttttgaatgg tttaagtgga gaagacaaga taaaaggtct
     6181 atttacagta cttcacatgg ataagaaatt tgttcatatg atgaacaatt tcgattactc
     6241 acttataaac ccaaaattga ttatatacat gtacaagagt attgtatttg ataaagaaat
     6301 agtttttctt atgcttttac ttagtaagat tggatttgat attatcatat tatgtccagg
     6361 aggagaaaac aatatagaga atgttataaa taaccaattg attgatatac acagattgga
     6421 taaaatggta tatgacctta aattaaattc gcttgaaaat gatatacctt tacttaaaaa
     6481 aatttttgga aagaggagac gattttagat ggggataaat ataaatagtt tttcaaaaga
     6541 taagaactct gtagaggtta ctgagaattt gcctacagca gttcaagaag aaaacttcga
     6601 tataatggaa tatacaaata ataaaaaaaa tgaactgaga aagtctaagg aagttgaggc
     6661 attgacatcc cttatagaag tagaaaatcc agatacgatt ttacagtttg gtaggaaagc
     6721 atctgaagga gtagcaagag tttctgatag ccttttaaat acaataaaat taaatagaaa
     6781 tgaagaaaat tctaaaatgc tggttcattt aactaaaata atggataagt ttgatttaga
     6841 cgattttcaa gaaactaagg aaccaaattt cgttcaaaaa cttttcaaaa aagctaataa
     6901 tgccattgaa atgatgtttc aaaagtatga aacattggga ggagaagttg agaaaattca
     6961 aattgagctt gagcaatacg aaagagatat agcactttct aataagcaaa taggtgctat
     7021 gttaaatgaa aactttgagt tttataatga acttcaaaaa tatattgttg ctggagaaat
     7081 ggcaatagaa gaaatggata atgaaatact acctttcttt aaacaaaaat cagagactag
     7141 tggagaccaa atggatgttg tcaattatca agaactatta aaagtttatg atatgttaaa
     7201 tcaaagagtc tatgatttaa gaatagcaga aaatatagct atccaaacaa tacctatgct
     7261 tagaggtatg caacataata attatggttt aattagaaaa ataaatagtg catttgtcgt
     7321 aactttacca gtatttaaac aatgtttatc tcaagctata ttacttaaaa agcaagaatt
     7381 acaagcaaaa tctctaaaag cacttgatga caaaactaat gaattattgc ttagaaatgc
     7441 acaaaatgta agtactcaaa gtgcacaaat agctagaatg gctggaacaa gttctgttca
     7501 aatagaaaca ttggaaaaaa tgtacaatac tatcaagtct ggtatagatg aaactatgag
     7561 aatagaagaa aacaatagag ctttaattaa ggataataca aagagattgg aagaattaaa
     7621 tacgactata atttataata agtagtatta tattaagggc taatttgtat aaagttggcc
     7681 ctttctatgt atatgaggag gctagacatt gaaatatttc gattatatct gcaaagatga
     7741 tttggagaga atttttttga aggagccaga agactttagt gctaagactg aaaaagatgt
     7801 gttgaaatat gcactgggcg cttttttata cgttccagct actcaatata atatgattta
     7861 taagagtatt ataggggatg ttaagggagt tagaccatta gctatttgtt tagaagatgc
     7921 tgttggagta aatggagagt tagaggctat agaaaatctt agattaattt taaaaaatat
     7981 tagcaatgaa agtataacta ataaagatgg aataccacta atttttgtaa gaataaaaga
     8041 tgtggagcaa ctgttgagaa ttaaagagat tataattaaa aatagtcatt ctataacagg
     8101 aatattaatt cctaaagcaa attcggagtt gattgaaaat tgtatagaag ctttatattc
     8161 tatgaatctt caagatatgt atgttattcc aataatagag acaagagaat ttatatataa
     8221 tgaaaaaaaa gaacttagtt ttactaattt gtacaatgct attttaagac ataagtcaag
     8281 aatattaagc attagagtag gcataactga tatattaggg atgtatggta taaggagaga
     8341 taaaaacttc tctatatata ataatttgat ttgttcttca tttatattag atataatcac
     8401 atatcttcaa agaccagagt tagatatacc tataagtggt ggagtatcag aattttttga
     8461 tatgacaaat aaggatataa gaaataagta tatagaagaa atattattgg ataaatatca
     8521 tggattaatt ggaaagaccg taattcatcc aatgcaaatt caaattgtac aagcgctatc
     8581 tacagtatca tatgaagatt ttactgatgc tttggatata ctggatagta cagacagtaa
     8641 gtatggtgtg agcaagggtg tattaggaga gagaatgaat gaaacaaatc cacatttttt
     8701 atgggctaaa aaaacattga ttctatctaa aatatatggt gtattaaata aaggagtaga
     8761 ctatgaagaa ttgcttaaat tttaattgtg agattgacat tattgataat ccattgaatt
     8821 tagaactaaa tgatttttta gacgtaggag taagaaataa tagcaaaaga cgttttttgt
     8881 ttatctcaaa gaaactcggt aaacatttag catgtaagcc aagtgagatg gataatttgg
     8941 gaaaattaat ggctacaatt tataatgaaa aaaataagga agaatcaaat ggtactgtta
     9001 tagcttttgc tgaaactggt acagctgttg ggcatagttt ttttgattac ttatgtggag
     9061 attatgaatt tattcatact actagagagc agtttgacga gttagaaaat ctggaatttt
     9121 tagaggaaca ttcccatgca acagaccaga atttatactt tggtatgctt aacaacttta
     9181 aatttggaga tgagataata ttggttgatg atgagattac cacagccaat acatgcatga
     9241 atataattag gaaaattcaa agcatttatc ctaaaaagaa atacactata tgctcaatac
     9301 tcaattgggt aagtgatgaa aatttaagta aagttactcg tttagaaaga gagctaaatt
     9361 gtaagataga ttttgtatac ttatttaaag gaaactttaa attttatata gatgaagata
     9421 ttatttcaaa ttacaacgat aattatagta ttactaaatt agagactatt tcaagtgata
     9481 aagtaaattt agtagtaaat catatttatg ttgatttaga agattatatt ggaaataaga
     9541 aatatgtaaa atatacagga agatttggaa ttaatagaag agaacaattt agattaaaag
     9601 aaatcgttaa gagagaaagt aaaaaaataa ctatggccag taaagaatct ttaaaaaatg
     9661 aaaaagaaaa gattctattt ttaggtactg aagaatttat gtatatacct atgctgtttg
     9721 caaaagaaaa agaaaatcaa tatgatatat actatcattc aacaacaaga agtccaatta
     9781 ttaatattga taagaagcat tatcctatta aaagtaaatt tatgctaaag agtttttata
     9841 atatagatat tcaaaattat atttacaata tagataaaca taatttttct aagtgttatt
     9901 tatttgtaga gctgaataaa agtgaaaatt cttatctaga atttattgat ataattaaaa
     9961 aaacaagtat aaaagaatta actatagtat gttgtagcta aagtgtttta acaggataaa
    10021 atatatcacg attttaagat aggagttgag tcatatgaga gattttagca cgtattctaa
    10081 agatgatgtt atttttttat taaaagatat atcaaattta attgaagaag aaggaaataa
    10141 agaaagagaa ttaaaaatac aaaatggtag acattattca gaaatgatac caatagagta
    10201 tgaagttagt gaagattatc tgaatttata tcatgagaaa ctaagagaaa ataaagataa
    10261 gcttgcattt tctataggag ttatgtgtga aaaaataatt aaaaaaaatg gaaatgatgt
    10321 agtactagtt tcacttgcta gagcaggaac acctattggt attttggcaa aaagatatat
    10381 aagaagcaag tataatgtaa gtttacctca ctatacaatc tctataataa gagataaggg
    10441 aatagatatg aatgcaattg aatatatagt aaagaatcat ccgagctcaa aaattcaatt
    10501 tttagatgga tggactggta agggaactat atcaaaagaa cttgaaaaag catgtgatga
    10561 acttaatgtt aaatttaaaa aacattttga ctcaacactt gctgtattag ctgaccctgc
    10621 tggatattca ggactttatg gaattagaga agatttctta ataccaagtg catgtttaaa
    10681 ttcaaccgtt tctggtttag taagtagaac tgtactaaga gaggatttaa ttggtaaaaa
    10741 tgactttcat ggagctaagt tttatagaca tttaaaagat aaagatgaat ctatgaacta
    10801 tatcgaaact atagaagaat gttttaaaaa tcaatttaag aatatatctg atgaagttga
    10861 aaactgggaa agtgacatta taacaagaga tggatacttt gatgttttaa atataaaaga
    10921 aaagtataac ataacagata ttaactttat caaaccaggt gtaggggaaa cgacaagggt
    10981 tttacttaga agagttccgt ataagattct tgttaaagac ttaaatgata aatctttaga
    11041 ccatatattc atattagcta aggagaaaaa tgttgaagtt gaacaaatgg atttaaaagc
    11101 ctataaatgt tgtggaataa taaaaaatat gaaagatatt taggaaatat attagacagg
    11161 agaatattga atgattgttt tttcagattt agacaggagt atcatttact ccaataaatt
    11221 tttaaatgct gatagtaaat atgctaatat agaaatttat agggaaaaag aaatatctta
    11281 tatatcttta gatactataa atttaataaa gcaaatacaa tattatggta tgtttatacc
    11341 tacaactact agaacagtgg agcaatttaa gcgtatagaa tttaataaat atgggattta
    11401 ttttccttgg agtataactt caaatggagg agttatactt aaagataatg aaatattgaa
    11461 atcatggagt gaaaaaatag ataaactaaa acctgattat gagcccattg aaagtatgat
    11521 tcataaattt aaagactatt tgaatgtaga tggaataact aattttaaag ttgcagaaga
    11581 tacatttttt tatatagttg ttgatttctc aagatttaat ttggattcaa tcaaagaata
    11641 tacaaatata ttggaatcaa aaaattggaa gttttatgtg agtggcagaa aaatatattt
    11701 tataccaaaa gaaatttcaa aagaaaatgc tataaaatac ttgactaaag aattaggaat
    11761 agaatatttt tatgcagttg gagattctat tatggactat ggtatgctta atatctcaaa
    11821 taaatcctat gtattgaagc atggtgatat aaataaaaat gaaatagaaa attcatttat
    11881 atcaagttct ttcaaaggaa tgagtggaac tgaagagata ttatctaaca ttttagacga
    11941 gaactgccta cttaatatat aatgactgat atataaataa taaactagaa aaatctgaaa
    12001 taaattatag taaagtttat catactaatt tatttcagat ttttatcaat ttatatttaa
    12061 gtaagaactc aatatgttaa aatgaaggtg gagtcattgc ccagactgag atacatacct
    12121 catcatcata attttcatac acatgtggta tagtactgtt aaaataaaaa ctatctcctt
    12181 cctccaatag atattcctta tttccaagtg ttatcttcat tttacctttt attatatagc
    12241 cacattcttc tccttcgtgt gtcacaaatt cactgttttt ttgtgtatga ccatctagcg
    12301 taacaagtat aaattctatt tttttcccag ataaattagg aacaagaagt tcataaaatt
    12361 ttgtagaatc attagttact attttttttc gattattttt ctttactact atatcagagt
    12421 cctctcttcc aatctcttca aaaaaataac ctatatttat atcaagagct tttgatattt
    12481 tccacattgc tacaatagat ggaacaacca tattacgctc aatttgactt attaagcctg
    12541 tacttaaatc tgaatttttt gctaaagtag agatacttat atttttagat tttctaagag
    12601 atctaatttt gcttccgaca tctatattca ttagatacaa accccctcag acatgattat
    12661 aacatataat ttataaagat taataaaata aaaaaaagtt ttataatgca tgtatttttt
    12721 atgttatatt gaattttctg aaaagatgca ttataatagt attaatattc aatatattga
    12781 atattgataa tgatatttta atttaatgaa atggaggtaa ttaagatgaa gagttttgta
    12841 agtcctgata atatttgggt attatgggca gttgtaactg gatgggcagc atttagtatt
    12901 tacttggaac aaaagtacaa ttgggcatca aaagtgtcgg gagcaataat agctcttgta
    12961 ggagcaatgt tattatcaaa tctaaatatt ataccagtag agtctgttgt atatgaccaa
    13021 gtatggggat atgttgttcc tttagccata gctttattgt tatatcaatg taatattaaa
    13081 aagatatgga aagaaagtgg aagattgctc attatatttc ttgtaggttc tgtaggaact
    13141 gtacttggag ctatgattgg atttttagca ttaaaaaatg tagtaccaga tttaaatatt
    13201 gttgcagcta tgatgacagg ttcttatata ggtggaaatg taaactttgc tgctatgtca
    13261 ggggcgtttg atgcgccagg agaattagta tcagcaacag ttgtagcaga taatctttta
    13321 atggcattat atttctttgt cttaatagct ataccatcaa ttggattttt tagaaaacat
    13381 tttaaacatc cacatgtaga tgaaatggaa agtataggta taactgaagg agaaacagta
    13441 gcagcaaatt attggggaag aaaagaaata tctttaaaag atatagcaat agccattggt
    13501 agcgccttta taattgtcgc agtatctgta gaactatcaa cttggtttaa agctataata
    13561 ccattatcaa atcctttttt agcaatgtta aatactttat ttggaaatca atatctaata
    13621 ataactactt tgactatgtt gtttgccaca tttatgccta atttctttgg tgatataaaa
    13681 ggagcacagg aattgggtac atttttaata tatatattct ttgttgtagt aggtgttcct
    13741 gcttcaataa cattaataat tcaaaaatca ccattactac ttttatactg cggaataatg
    13801 gtactaataa acatgctagt tacatttata gttgcaaaaa tatttaagtt tagtttagaa
    13861 gaagctattc ttgcatctaa tgcaaatatt ggtggtccta caacagcagc agctatggca
    13921 atatcaaaag gatggtctaa gctagtaggg ccaatactta tagttggaac atttggatac
    13981 atagttggta attattttgg tcttttagta ggaaatattt tgatataatt ttacgtgaaa
    14041 agaagttgtt ttttagaaag atttttattt aattggaggt tgttatgatt ttcgatggac
    14101 attcagatac ttgggcagat gtaacaataa gaaggcttaa cggggaaaaa gatgtattta
    14161 gaagaatcca tcttgataat tttaaaagag caaatataga aggagctatt tttgtaatat
    14221 ggatagaccc accatatgat aaagagcctg aaaaaagagt taaacaaatt ataagctcta
    14281 taaaagatga aattgaagat atgaaagatg ttgtaaacat agtaaaaaaa tattcagata
    14341 tagataaagg tataagtgaa aacaaaataa atattttaat tggtcttgaa ggattaagcc
    14401 atattggaag aaatatagat ttactagatt atttttataa agaagttggt gcaagacatg
    14461 catcattaac ttggaatgaa cagaatgaac ttgctacagg atgtttaggg gatgaaaatc
    14521 gtggtttaac tgaaataggt aaaaaagcag tcaataaaat ggaaaaattg ggcatgatag
    14581 tagatgtgtc acatttaaat gaaaaatcat tttgggattt aatgtctgtt gcaagtaagc
    14641 cagttatagc ttctcattct aatgctagga gtttatgtga tgctaaaaga aatcttacag
    14701 ataatcaatt aaaagaaatt tcaaatacag gtggattagt tggaataaat agttttagag
    14761 gatttactaa tagtaatcct aagaagcaag atttaataca tttggttgac catatagact
    14821 atatggtaaa tcttataggt atagaacatg tatcttttgg ttttgatttt agtgattact
    14881 tagggaaaga gactataggt tcttttactg gaaatgctag tgtgccaagt gtagatggtc
    14941 ttgaaagcac acatacagca cagaacatag tagtagagct tgaaaataga ggttataaaa
    15001 agaaagatat agagttaatc tctagtggta atttttatag gctctttaaa gatatactaa
    15061 aataatataa taaagaactt aaatttataa aatagtatat ttatacaata atatctacat
    15121 atttaaattg gcgttcataa tttattaaat taaattttaa tgttactaga agtttcaatt
    15181 ctagtaacat tttttatgtt aaaaatagat atagatatat attgacaatg ataattatta
    15241 ttgataaact ataaaagtga aaatgataat aattttcatt ttaattaata aaagattttt
    15301 aaggagggag aatgaagaaa aggtatttag tcataggaat aataattttg tcttattttt
    15361 ctttatttat aggagctgaa gatataaata taatgcatat atttgcaaaa aatcagcata
    15421 agcttatgat atttatcatg agtagggtac caagattaat aagtatattg attgctggtg
    15481 taggaatgag catagcaggt cttataatgc aacagataag taagaataaa tttgtatcac
    15541 ctacaacagg tgctacaata gatgcagctc aatttgggat agtaatatgt atgcttttag
    15601 taccaactgc ctctattttt acaaagacaa taatagcatt tgtattttct cttgtaggga
    15661 cttttacgtt tatgaaaata atagggaagt tacaatttaa aaatataatc tttgtaccat
    15721 tagtaggtat aatgtttgga aatataatag gttctatgac ggattttatc gcgtataaat
    15781 acgatttgag tcaaaatgtt agttcttgga tgcaaggtga cttttctatg atacttaaag
    15841 gaaattatga gatattatat atcacaatac cccttataat attagcatat atttatgcaa
    15901 ataaatttac tgttgttggt atgggtatgg attttgcgac aaacttaggt cttagttaca
    15961 aaagaattgt caatataggt ctgataatag tagcacttgt tacagtatgt gtagtcgtta
    16021 cagctggtaa tatccctttt ataggtctaa tagttcccaa tatcgtatcc ttgtatatgg
    16081 gtgataatat aagagcaagc atatggtata ctggtttact tggagcggta tttgtactta
    16141 tatgtgatat ttttggaaga attattattt atccatatga aatatctata ggacttacag
    16201 ttggagttat aggaagtata ctattccttt atttaatatt aaggaggaat gtgaatgaag
    16261 cttagtctca atcaaaaaat ttactttgta agtatattac tattcatatc aatagcactt
    16321 tttataattt atggaataga catgaatcat ttagaatatg cattatcaca aagaatacca
    16381 aaaatatttg ctatggtatt aggtggagga tgtatagcat ttactactgt agtgtttcaa
    16441 acaattacaa acaatcaaat attaacacca agtgtactag gtttagattc attgtatgta
    16501 atgattcaaa ctataatagt attcatattt ggttcaagca gtagtctgat tataaatgaa
    16561 aattataatt ttattattaa tgtagctatg atgattggag cttcattgtt attatataag
    16621 atgttatttg aaaaaaataa aaataatatt ttctttttaa tcctagtagg tatgatattt
    16681 ggaaccttat ttaaaagtgc aacaacattt attcaagtaa tgatagaccc gaatgaattt
    16741 ttagctttac aaactagtat aatggcaagt ttaaacaata taaacactaa tgtattgtta
    16801 atagcattta taataattat tgccataata ccttttatat atgatgaaat aaagtattta
    16861 gatgtattgt cgttaggtaa agaacaagca attaatttag gtgtagattt tgataaggca
    16921 gttaaaaaaa tgataatatt aatagcaatt ttagtctcaa tatcaacagc acttattgga
    16981 cctatgacat ttttaggact tttacttgct aatataacaa gagaaatttt taaaacatat
    17041 aagcatacat acttgatttc gggttctatg ttgataggga tgattacatt gataataggt
    17101 caatttttta tacaacatgt atttaagttt gatacgactt taagtgtagt aataaatttt
    17161 ataggaggaa tttactttat tcaattacta ttaaaggggg caaatagatg atagagataa
    17221 aaaatatatt taaaagatat aagaataaaa atgttgtaga tgatgtctct tttagtatag
    17281 aaaagggaaa gataacatct tttataggac caaatggagc tggaaaaagt acagtactat
    17341 caatagttac aaggcttata ggaggtgatg gcggagaagt aataatagaa ggaaaaagct
    17401 tgactaatta tagcaataaa gaattagcaa aaaaaatagc tattcttaaa caatctaaca
    17461 atataacttt aaagcttaca ataagagaat tagttggatt tggaagattt ccatatagtg
    17521 aaggtaatct aactaaagag gatgaaaatt atattgatga agcaatagaa tatatgaagt
    17581 tgactgatat acaacataag tatttagatg aattgagtgg aggtcaaaga caaagagctt
    17641 atatagcaat ggtaatagct caagatacag agtacatatt acttgatgaa ccactgaaca
    17701 atttggatat gaaccattcc gtacagatga tgaaggtact tagaagtctt tgtgatgagt
    17761 tagataagac tatagtatta gtaatgcatg atataaattt tgcatcatgt tattcagata
    17821 atatagttgc attaaaaaat ggaaaagttg aaaaagtagg tcgtacagat gagatagtaa
    17881 atgaaaaagt tttagaagat atttatgaga tgaattttaa cattaagaat ataaatggaa
    17941 atcgaatctg tatttatttt taaaatattg tatatgaaaa taagcttgct atatgatttt
    18001 ttataaagtt tttaaattaa aataaagatt aataggagag aggaaaatat tgatatgaat
    18061 aaaaaagcag caatagtagc agcagtagct attataggat tagtgacagt gtttgcatta
    18121 ggtggaagta aaaagaatga atcaaaaaca tcagaaaatt caaataatac cataaagata
    18181 actcataact tgggagaaac agatgtaaaa ttaaatccta aaaaagtagt agtatttgat
    18241 tattcggctt tagatacaat ggatgcatta ggtgtagctg agaatttagt aggacttcca
    18301 aaaacgagtt taccagcatc tttagaaaaa tataaggata agaagtatgc agatttagga
    18361 ggtttaaaag aaccagattt agaaggtata aagtcggcaa atccagattt aattataata
    18421 aatggaagac aagaagattt ttatgaacaa ttgtcaaaaa tagcaccaac aataagtaca
    18481 agtaaagatg ataagaagta cttagaatct gttaaaaata atatagacaa aatagcaaaa
    18541 atatttggag tagaagaaaa agcaaatcaa gaatttagta aaattgaaaa gaaaatagaa
    18601 actttgaata aaaaagtaac agataaaaat ttaaatgctt taactataat ggtaaatgaa
    18661 ggtaatttaa gtgtatttgg tgaagaatca agatttagta tattatacaa tagttttgga
    18721 tttgaaaata aagataaaaa tataaaagaa tcaagtcatg gacaaaatat tacttttgaa
    18781 tatatagcta aacaaaatcc agaagttatg ttcgttatag atagaggaat agctactggt
    18841 agtgatgtaa aagaaagctc aactgcaaaa tctgttttga acaatgatat aataaaatct
    18901 atggatgcat ataagaatga taatataata tatttagatt caccaacatg gtatgtaaat
    18961 gatggtggtc taacatcttt aaataaaatg atagatgatg cttcaaaagc ggtaaattaa
    19021 taatccgata taatcaaaat gtattttttg taaaatgatt atcaaataaa ctaggagttt
    19081 ttaagaactc ctagtttatt ttttg
//
LOCUS       DAEPOZ010000050        18825 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.050, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000050 DAEPOZ010000000
VERSION     DAEPOZ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18825
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.050"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(130..321)
                     /locus_tag="KR440_001392"
     CDS             complement(130..321)
                     /locus_tag="KR440_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium protein"
                     /protein_id="HBH0698449.1"
                     /translation="MAGLTTEPGGGGVLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQ
                     SAGTAATLTIFRIADTVMT"
     gene            complement(490..1083)
                     /locus_tag="KR440_001393"
     CDS             complement(490..1083)
                     /locus_tag="KR440_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HBH0698450.1"
                     /translation="MRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYY
                     NLRTITWNDRVSMENIRGYLPIGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNY
                     PYQIFEFTSSRDCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSIGPMG
                     ATGPTGATGNSSQPIANFLVNAPSPQTLNNGNAITGW"
     gene            complement(1084..1230)
                     /locus_tag="KR440_001394"
     CDS             complement(1084..1230)
                     /locus_tag="KR440_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium protein"
                     /protein_id="HBH0698451.1"
                     /translation="MRKIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFF"
     gene            complement(1908..2432)
                     /locus_tag="KR440_001395"
     CDS             complement(1908..2432)
                     /locus_tag="KR440_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBH0698452.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQIMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(2497..3249)
                     /locus_tag="KR440_001396"
     CDS             complement(2497..3249)
                     /locus_tag="KR440_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBH0698453.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDISKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(3280..4578)
                     /locus_tag="KR440_001397"
     CDS             complement(3280..4578)
                     /locus_tag="KR440_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HBH0698454.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEDIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMEDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQQGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREVNVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(4595..6859)
                     /locus_tag="KR440_001398"
     CDS             complement(4595..6859)
                     /locus_tag="KR440_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HBH0698455.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(7114..7932)
                     /locus_tag="KR440_001399"
     CDS             complement(7114..7932)
                     /locus_tag="KR440_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBH0698456.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGIDEKIIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIIL
                     LDEPTAGLDPVSTRSIVDIIKGLNKDNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFVNENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(7925..8623)
                     /gene="cbiQ"
                     /locus_tag="KR440_001400"
     CDS             complement(7925..8623)
                     /gene="cbiQ"
                     /locus_tag="KR440_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HBH0698457.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIKNIFVLSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEIFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKMHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(8626..8910)
                     /locus_tag="KR440_001401"
     CDS             complement(8626..8910)
                     /locus_tag="KR440_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH0698458.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPDYEPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGVQKGKRS"
     gene            complement(8900..9652)
                     /locus_tag="KR440_001402"
     CDS             complement(8900..9652)
                     /locus_tag="KR440_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HBH0698459.1"
                     /translation="MKQNIKLGVIAALMLIVLTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLVGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIISFLIFKALKKKDG
                     NNAMPVFLAAAIGDLATYTVTSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(9841..10033)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(10165..11937)
                     /locus_tag="KR440_001403"
     CDS             complement(10165..11937)
                     /locus_tag="KR440_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HBH0698460.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYNMDKHPALDSMTRRIVNDIRNIDTN
                     QDMMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(11951..12880)
                     /locus_tag="KR440_001404"
     CDS             complement(11951..12880)
                     /locus_tag="KR440_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0698461.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            13109..13297
                     /locus_tag="KR440_001405"
     CDS             13109..13297
                     /locus_tag="KR440_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0698462.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRAKRRKEVQYE"
     gene            13290..13991
                     /locus_tag="KR440_001406"
     CDS             13290..13991
                     /locus_tag="KR440_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0698463.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLLDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSINNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            13993..14916
                     /locus_tag="KR440_001407"
     CDS             13993..14916
                     /locus_tag="KR440_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0698464.1"
                     /translation="MNTSVYLLLILALSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            15073..15996
                     /locus_tag="KR440_001408"
     CDS             15073..15996
                     /locus_tag="KR440_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0698465.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGCEKRVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDHTLRLYNTTLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            15989..16726
                     /locus_tag="KR440_001409"
     CDS             15989..16726
                     /locus_tag="KR440_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0698466.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPTKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFASIALGRRK"
     gene            16737..17510
                     /locus_tag="KR440_001410"
     CDS             16737..17510
                     /locus_tag="KR440_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0698467.1"
                     /translation="MWKLLWAECQKIRRSKIVWITFFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETKLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            17526..17678
                     /locus_tag="KR440_001411"
     CDS             17526..17678
                     /locus_tag="KR440_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698468.1"
                     /translation="MKKIKKLIILIVFLFLFGMLHCYTEWKFNNDPPKGVIERINEWT
                     DKIGLD"
     gene            complement(18051..18659)
                     /locus_tag="KR440_001412"
     CDS             complement(18051..18659)
                     /locus_tag="KR440_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBH0698469.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         6095 a         3238 c         2338 g         7154 t
ORIGIN      
        1 atttttatga atatatttta ttattattgt tattttattc attctaaaat aggtaaaact
       61 caatatataa aaatactttt gtcatttaaa taaaacctaa atagttttta agattatttt
      121 agacacgttt taagtcataa cagtatcagc tattctaaat atcgtcaaag tagccgcagt
      181 tcctgctgat tgcccatttc gtatccctac tgttgtagat gaaaaattat ttattgtaaa
      241 agtagtcccc ccaaataaaa aaccagcaaa atatccagaa agtactcctc cgcctcctgg
      301 ctcagtagta agccctgcca aatcttttcc tcctaaaatt gggaatagga tagcaaaaga
      361 atattgattt atacttgatg aacctggttg taatgctact gaaactgata tataatacac
      421 accattttct tgtactgtaa acgtaccatt tgcatctact gttatacttg aactatttcc
      481 tattattgtt taccaacctg ttatagcatt tccattattt agtgtttgtg gagatggtgc
      541 atttacgagg aagttagcaa ttggctgaga ggaattgcct gttgctcctg ttggacctgt
      601 tgctcccatt ggtcctatac ttcctgtcgc tcctcttggt cccattggac ctgtaattct
      661 aattggaaga cactcacatt taggataaca attatctata attctatctt caaatgttac
      721 aagtatatac ggtttgttac aatccctact agatgtaaat tcaaaaatct gataagggta
      781 atttaaagat aaagctaacc cataattagg atatttattc attgcccatg cttttatagt
      841 tcccgtaata tttaaacaaa tatagttgga tgtatcactt atcccaattg gtaaatatcc
      901 cctgatattt tccatagaca ctctatcatt ccatgttata gttcgtaaat tataatattc
      961 acttattctt ttaatttcaa tatctacttt ttctttaaaa atatgattct tatttgaatc
     1021 aatatgaata aataactctg cttttaatat acttttgtct tttaatatat ctggtattct
     1081 cacttaaaaa aaagtaatca acttttcttt tacaaaacta tttgaacatt tacttcctat
     1141 taagcaataa tctaaattac taaagttttt atctggatat tttttagata taaaagtatc
     1201 atcatttaaa taaagtataa tttttctcat ttataatccc tccattttgg gtctatatca
     1261 tactatgttt aaaatacatg cttagttaca atacaattaa aatttatagt attaaatttt
     1321 tatataataa tgctgatttc tacaaaaaaa gaacatgtca ttagaaactt ctaatagcat
     1381 gttctttttc attattatat attacacttt aaattattcc ttgactggct tagctggtgt
     1441 aaactaaacc agtctttcta aaatattaag cacttttttc ttattatgtt ttttaggatt
     1501 tattgtctct attattgtaa accaaagtta taaaattgta aatagaatat attaccataa
     1561 caatattttt ttataagtaa aaattataaa aaaccttatt tttattaaat agaataaata
     1621 aaataagttt aaaaaatact gaaataagtg ttttccacat attttatata tttatttaat
     1681 acattattat ttaaaaataa atatataaac aatcataata aaattttttt aataaaactt
     1741 tattttttag ttaaaatcat acaatatata gctttccaca atacaaataa attaaaaagt
     1801 gaaatgtatt tgtctaaaaa cataataaca tttcacttta tcctcatttt taagtaatat
     1861 atatttttaa tttttattta taactcaatc tagtttttat ataagtttta gtttttttct
     1921 acatctgagt aatctattcc aaaagtttct acagtaacag acttcataat ttgtggagtt
     1981 tgtggcttat cagctgaatc tgttctaact gatgctattt catcaactgt atccatacct
     2041 tctatcactc taccaaaacc tgcatactga ccatctaagt gtggtgagtt tttatgcatt
     2101 atgaaaaatt gagaaccagc tgaattaggt gccatagttc tagccattga tatgactcct
     2161 ctttcatgtt ttaaattgtt agtaaatcca ttaccactaa attcaccttt tattgagtga
     2221 cctgggccac ccattcctgt accatttggg catcctcctt gtatcataaa tcctggtatt
     2281 actctatgaa atattattcc attgtaatat ccttcattta ctaatgatac aaagttttta
     2341 actgtatttg gagctatatt tggatataac tcaattttga tttcttttcc attttccatt
     2401 tctatagtta ctataggatt tttattttcc ataatttatt accttccttt tcattagtta
     2461 aattacataa ttacatttat tatattatac tctaaattaa agtctatcaa gtatattttt
     2521 tcctatttcg tatccttctt gataaatagt tttacaaaac tcatcatcaa agttaagcgt
     2581 atctttagga gcaaattgag tattaggtct aactactatt attttatctt catcatatac
     2641 ttttttaata tcctctggca actcataatt atattttgtg gatattaaaa taattttatc
     2701 cacattatcc ccaattaatg gttcaattaa tgcactccta attaaacctc catctaattt
     2761 gaaaccatca taaattcctt cttgcacatc tttcacaaat tgttctttaa atccaagagt
     2821 tgcgtttgga tttgatggta acaacccact gtatgaaatg ctgataatgg cttcattacg
     2881 aggcattttt gatatgtcaa caatcttttc tgatactact ttattatcaa cttcaacata
     2941 attaacataa aagttcattt cttctatatt actatcatct agttccctta ggttgtctat
     3001 agcaggagaa ttatccacag tattatttac tatttttaca tttccattct ggatatcttt
     3061 tatatttgtc cacattttct ccaagttatc aacattccct gtgtacaaat aatatccatt
     3121 tattgctcct attgatgtcc ctgaaatcga ataaaattta tttattcctc catcatacaa
     3181 ggctttaact actcctgctt gataagcccc ttttgctcct ccaccttcta aacataatcc
     3241 taatttcata gtctcatctc caaattgttt atcttatatt tatatttcaa aaaacttact
     3301 aaatgtttta tatatactat agtggtcctc aacatttcct aattctctta tagagtgcat
     3361 tgctaaaatt ggacttccca catccacaga tggtatatct atatgagtag aagatattgg
     3421 acctatagta cttcctcctc tttcatctga cctattaaca aattcttgat actcaacatt
     3481 aacttctcta catatatttt tataaatagc tatagaatat ccatcactag tatatgcctg
     3541 actagcattt atctttataa ctggaccttt tcccattatt ggtttattag ttgggtcatg
     3601 cttttctgga atgtttggat gaatagcatg agctaaatct gatgatatca taaaagatga
     3661 ataaatagac tcaaagaatc cctctctatc cttacccatt gatatgcaaa ttctctccaa
     3721 tatatttaac aacatgtttg aatctgcacc ttgtttagta gagctaccaa cttcttcatt
     3781 gtcaaataca gccattacat ttacaccttg ttgaccttta gtattaacaa gagcattcaa
     3841 actagcatgt accattgcaa gattatcttg tctcccaata gatataaatt catcattagc
     3901 ccctattaaa cttccttttt caaattcata taaaaataaa tcaaaatcta taatatcttc
     3961 catatttaca tctaagttag atgctatttg ctttataaga aaattatctt tttctaatgt
     4021 atcatttaac atcccaacta aaggtaacat atctttttgc ttattatatg aatgaccatc
     4081 atttatactt ctatttaaat gtattgctag atttggtatt atacaaattg gctttttaaa
     4141 gtctataagc ctttcatttg gttttaatat actatcacct tttaaaacta ctctaccagc
     4201 tatcccaaga ggtctatcca accatgtact taatattgct cctccatagc attctgtatt
     4261 taacttcaaa taagattttt cagcttctat ttctgcattt ggtttaatac taaatgtagg
     4321 agaatctgaa tgagagccta ttattctaaa tccatctctt tctatatctt ctgaatttac
     4381 tacaaaagct actatagctg atagattctt agtaacataa tactttccac caactcttag
     4441 attccatctt tgattcaaaa ctaattcttc aaatccattt ttatctaaga tttccttagt
     4501 actctcgact gcatgaaaag acgacggact atcatatatg aagtctatta agtttttagc
     4561 aaagtttata ctatccattc ctcatttacc tccattataa aatatttagt gttgtatatt
     4621 ctttatttat ttttttaatt ccctgttggt caaaagctat agtaacatct gtaccaatta
     4681 tagaaaccac tgtcccaacc ccaaatttag gatgatgtac cttagaacca agttttatat
     4741 catctggatt agtttcttta ttagaatctt ttattgtagc attaacttta tctgcaactt
     4801 tagatttgct tatagtactc atatactttt tagtatattt atctaacata ttataacttg
     4861 ctttagaata gcttagttct ttttttactt tgtacaatct ttctatacat tcttctggca
     4921 gttcttccat aaatcttgaa gctattgcaa catttgtttt accatataat gttctcttct
     4981 ctgtcaatga catatatagc tcttctttag ctctagttat accaacataa caaagtcttc
     5041 tctcttcttc tatttgtgaa tcactcatag attttatagc tcttgatata ggaaataacc
     5101 cttcttccat acctgttaaa aacacaacag ggaactctaa acctttggat gtatgaattg
     5161 tcataagcga aaccctatca ttgtcttctt catcatttga ttcagatgta agtgctatac
     5221 ttgttaggaa tgtctctaag tctttatttt cataagtatc atcattacta ctttcaaact
     5281 ccaaagctat agaaataaat tctctcaaat tatcaattct gtcttgagct tcttcacctt
     5341 tgcctgttaa atctttttca tttttatttc ttatctctac aagctcgtcc atatatcctg
     5401 tagtatccaa gactttttct ataagtttgc taacaggata aacttctttt atagttctaa
     5461 gagtccctat aatatcaaca aatccactta tacttgccct tgccttagta gatatatctg
     5521 aatttgtttc tatatcaatt aatactgagt atatactttc ctgttttaag ttagctctat
     5581 cttcaatttt ttctatagtt ctaagcccta tacctcttct aggaacattt ataatccttt
     5641 ttatagatat atcatcttgt ggattttgaa taactcttaa atacgccaac aaatccttta
     5701 tttcttttct ctcgtagaat tttgtaccac catatatatt gtatggaatt tgacttctat
     5761 ttaaagcgtc ctctacaggt cttgcctgtg catttgctct ataaagtact gcaaaatcct
     5821 tataaggtct attttctttt ctagctattt gtgcaattga atcagcaatg aaatcagcct
     5881 cttctatttc gctaccagtt aattgtatct ttataagttc tccttctttt ttttcactcc
     5941 aaagcttttt tctttttctt tctatattat tagatataac tttattagct gcatctaata
     6001 tcacttgtgt agacctataa ttttgttcta actttacaac atgtacattg tcataatctt
     6061 tctcaaattc aagtatgttt cttatatctg cgcctctcca tccataaata ctttggtcat
     6121 catctccaac aacacaaata ttttgatgtt ctcttgctaa taattttatc aattcatatt
     6181 gagctttact tgtatcttgg tactcatcta ccattatgta tctaaatcta cttctgtaat
     6241 atgctaatac ttcatcatta gccttgaaaa gttctacagt tttaagtatt aaatcatcaa
     6301 agtcaagtgc actatttctt tttaaacggt cttgatataa cgcataaata tctgcaatct
     6361 taaccattct attatctgcc atgttgatat ctttaaactg ctttggagta taaagtttgt
     6421 cttttgctcc tgaaattgca cttataactg cttttggttc aaacacttta tcacttaaat
     6481 ttaattcttt tagacagtct ttaaccaatg taacttggtc tgctgaatca taaattacaa
     6541 aacttctatt gtaaccaatc ttatttatat cttttcttag tatacgaaca cagcaagaat
     6601 ggaatgtact tatccacata tccttagttt caggacctat attttgttca actctctctc
     6661 tcatttcatt tgcagctttg tttgtaaatg ttatagctaa tatatttggt gcttgtacac
     6721 ctttatcttc tataagatat gctattcttg ttgttaatac cttagtttta cctgaaccag
     6781 caccagccag tataagtact ggaccttcag tcttttctac tgcttctctt tgagcaggat
     6841 ttaatgtatc taaattcata tttctcctcc tgttaacagc cataaatata ttttaatcta
     6901 aaatatgaat tacttcaaat tataattatg tattatatta atagattatt tccaatatat
     6961 tacaattaaa taacattttt ccatataatt tatttaattg tttaataaaa aaaataacct
     7021 tatgatttgg ggaatcataa ggcaccttta aggggtctag gttaagggtt attatatata
     7081 aatctatttt tttaaaatag gtttagggct taattattta tttgtattaa gttctctttc
     7141 cttccaatac ttatacaaat cttcttcttt cttaaataag ggtaaattca tatttctatg
     7201 tacttttact aaccatgggg attctaggcc agcttgtata atgttatttt catttacaaa
     7261 tacattttct gcctttcctt catcaattaa aatcccctta tccaatacat atatataatc
     7321 gcatatttca tacattaaat tcatatcatg actggatatt actattttta tattatcttt
     7381 attcaatccc tttattatat ctacaataga cctagtactt actgggtcta aaccagctgt
     7441 aggctcatca agcaatataa tttcattttc cattgctata actgatgcta tcgctaccct
     7501 ttttttctga ccataactta aaaaatgtac tggtctatct ataaaatcta ttccattaac
     7561 agctactaga gctttgttta ttctctcttt aattattttt tcatctatac caatatttct
     7621 cattgcaaag gctatatcat catatacttt tgaataaaaa atttgttttt ctgggtcttg
     7681 gaacacaata cctacatttt ttctaaggtt gtataaactt ctcttatcat attttaactt
     7741 ttcttcttta aaaagaattt ctccagatgt aggctttaat atacccatta aattcataaa
     7801 taatgttgat tttcctgaac catttgaacc tattatacct ataacatttc cctttgaaaa
     7861 atccatatta atatttaata gtgcattggt attcttttca tattgataag ttaaattatt
     7921 tattttaaac atcattatcc cctactatgt gaaatttacc atcatacaat tttatgtcta
     7981 atgatataca catatcatca taccttgtca taagtctttt gtacagcata ctgccaagaa
     8041 ttcctaatga tttatatgaa gtttttaaat ttatgtaccc aaatcttagt tcttgtgact
     8101 tatatatttc tgaaaattct tctaaaaata tgaatataaa cctatatatt aacattgata
     8161 gctcaacaaa agtatcaggc aaatgcattt ttttaaagaa gaatattaat tgattaaaag
     8221 gtgttgtaag tatacaaaag tatacacaag tcaaacaaga catagctcta aataacacat
     8281 gaatagacat gtcaatagat gcttttgata ttcctacatt gaaactaaaa atttcaaaac
     8341 tatataataa gtctgcctta ttaaaagata tatttacaag agttattcct atactaagaa
     8401 ataaaaagta cattggtatt ctgagtaatt ttaaataatt ctttaaatct attccagcta
     8461 cacaaacaac tagaatactc attaaaatca taattccact taaaacaaat atatttttta
     8521 ttaccataga agcaatcaga aagattactc ctatggctac ttttttattt ggatttactt
     8581 tacttaatct atttgtatat gcatatttat caattataag cataattaac ttctctttcc
     8641 tttttgaaca cctaatatgt accctattac accagcacct aacgctgctt gtgcagagaa
     8701 taataaactt tctatttctc cacttggtgg ctcatatggg ctactaaacc atggttcata
     8761 atctggattt atcttagtaa tttcactctc agcttgacca tctgctcctc catattcagc
     8821 tcccgaatta acaagtaatg ggaatatgat aagagctact actaataaaa gtagtaatat
     8881 attagttttc gtctttgttt tagcactcat tagctttaac tcctttatca ttattttcta
     8941 gtatgctttt ttctccattt tctgtaatta aattatatac aattacagta agaataccct
     9001 ctgctattgc tattggtatt tgagtcataa agaaaattcc taaaaactta attgctgaag
     9061 ccatcactcc acctgatggg tctgggaaag ccagtgctaa ctgtatcgaa gttactgtat
     9121 atgtagccaa atctcctatt gctgctgcaa gaaatactgg cattgcatta tttccgtctt
     9181 ttttctttaa tgctttaaat attaagaatg atataattgg tcctataata gccattgaaa
     9241 aagcattagc tcctaaagta gttattcctc catgtgccaa tagtaatgct tggaatatta
     9301 gaactatagt tccaagaaca aacattacac ttggtccaaa cattattgct ccaagaccta
     9361 ctccagtagg atgtgaacaa cttcctgtta ctgaaggtat ctttagtgct gacaatacaa
     9421 atacaaatgc tccacacagc gctaataata ctttcttttt agggtcttgc tttactattt
     9481 ttcctattga ttttagtcct actaaaaaaa atggtataaa tataactccc catgctatag
     9541 accattttac tggtaagtat ccttccatta tgtgcattgc attcgatgta actggagtta
     9601 aaacgattaa cataagtgca gctataaccc ctaatttgat attttgtttc attttactat
     9661 cccctctctc cctttctaat aattaaaata taaataatat atgtctgatt tattttaaaa
     9721 ataaaaataa aaaagcctaa ctcgatggct aggcttctaa ttttcgataa tccaaataca
     9781 aacacataaa atgaacatat ctgtataatt attataagaa actttctcca tccccgaaga
     9841 aattacttta atattttagg caggtctcct gacttagctt cttcctactc ctcttccttc
     9901 ccatgagata taaaaaatat catcatagtg gctaacaaaa gtttcgtctg catcacagta
     9961 gcgggggctg taaaggaatt taaccttttt ccctattaat cttgcttaaa gaacctataa
    10021 tattatatat ttaattttta aattattctt aaatcatttt ctatataaat cattgtatac
    10081 cttttctgtg aaaatttcta cattttttat aaattttatt catttttata tttttatgta
    10141 tataaattct tttttgtaag tttattaatt tctaattatc ttctttaaat acattgaaac
    10201 tacatcagaa aatctagtta tatctcttga atatataaaa tcatttccat atattaatct
    10261 ttcagcttca agttctgatt cttttcctgt aaatactcct agtactaata tattttgctt
    10321 tctagctttt ctcacttcat tggctgtatc tctcaaacca actatacccc tataagcctt
    10381 ttctcctctt aatgttctct cactactttt tcctatcttt acatcatttg gtcttccatc
    10441 gctgagaact atcaggattt tattttcttc atctctattt attagaccac tacatataga
    10501 ttttattgcc aaaccatctc tattattacc agcacaaaaa tattcaaata tattttcact
    10561 attttttata ctatcatcat aatctttaaa tctttttaat attgtatagt ctaaaaaact
    10621 agaaaatccc atcactctat ttggtatgcc aacttctgtt agagcatttg caattatgta
    10681 agcttgaatt gccacatttc cctgatttct actttgagag ccacttgcat ccaataatat
    10741 atcaataacg tatttccctt tttcattttc tatatcttta taaaatattt tattattatt
    10801 acttctacca actctccaag ctctatttgc taatatagta ccacaatcag aatatactct
    10861 acttttagca ttttcttcta ttaaaattct tgacagactt tcttttaatc tctttatttg
    10921 ctgtctatac attctagcat gttctctaaa ttttcctaaa ttattttctt tttgtcttgt
    10981 tacatatttt agttgaaata cattattaca ctcacttcta agaactccat ctgtaaagtg
    11041 tattctacaa ccttcatgaa catttctaca attttgtgtc tcaatttttt taatttcacc
    11101 tttactcaaa taagtttttc cataataatg ttctattttt gaatatatct tatttgcagt
    11161 gtcttcatca acatatataa ctcggtctgc tgaattgcta catttagaat catcaaaatc
    11221 ccctacacct tctacaagca tggatgaaga tattgaatct atatcacttt caataatatt
    11281 ttcttcttca tcactataaa gttcttcata catgaaatca gcaaatgtat caaaatttac
    11341 ttctacactt tctacattat tttcatcaat aagactattt tcttctccat ttaatatgta
    11401 ttcgaaataa gttaaatata taacatctaa tgcttttaaa atctccatca tatcttgatt
    11461 ggtatctata tttcttatat cattaactat tcttctagtc atactatcta atgctgggtg
    11521 cttgtccata ttataaagta caaatgtgta tttaatttta tccataatat tatcattgtg
    11581 tattttggta aaacgtgaaa caatatcttt ataagcattt cttcttatgt cttctatccc
    11641 tggcctatct ttaatgacct tttcttcaac tattgtattt aatataactt gaattagacc
    11701 cagtataata tctttgttgt atccttttct tattctattt gtaagatatt ttttaactat
    11761 atcccaatct acatactttc ttctagcacc tgcaattatt gcaaaatata gacctatttc
    11821 ttttgaagag taatcttttt ctgatatatc tatactttca ttgtaatcac cagctattgt
    11881 ccatgataag ttattagctc tattttcaaa ttcataatca tcatatatcc atttcataat
    11941 ctcacctata ttaaatatga cttattggaa atacatctat actctcccat ttattaggta
    12001 ttctagtttt aattacatct ccaaccattt ctttctcata tacatcaaaa gtctttcctg
    12061 taagtcccat attaattgca agagttggtt ttaatcctct tcttatagtc ttaagagatg
    12121 ccataagacc acgtaaatca atagctttac tagatatctc tccattttga gacttcaatt
    12181 gtaaatctaa aaatattcct gcaaagtgta ttaatttttc ttcatcagca tctgaaaatt
    12241 cattttttaa aatcatcatt aacttatctt cttctatagg aggaatatct attaccataa
    12301 atctagatac cagtgcttca ttaagttctt ttgttcctgc atattcataa ttcatagtcc
    12361 caataaatct tgttgctgga tgtagattga ctctctcata cccaggtaca tcaattattc
    12421 ttctatagtc taaagctgaa tgtaccacaa ctatagcatc attttttgcc atattaattt
    12481 catcaaatac tccaaaacct ccattaatag cacactcata aactgagcct cttctaagtt
    12541 taacttcatt gtctataaat gtatctgtac ctataagtgt tgaactgtct gtatttatat
    12601 gaaatgatgt attccactga ggtctattaa atatttcccc tagattatca gcaagtaaat
    12661 tttttcccgt tgcctttggt cccgataata atatattttc ttcttctaaa atagctgcta
    12721 tacacataga caagatatct ttaccataaa aataagtttt agactttgtt actctgtatt
    12781 ctaaatcttt ttctaaacca taataatttc taaaatacag tacatcttca ataagttttt
    12841 catctacccc ttgcaccctt agattatcta ttatatccat atttttaccc cttttatata
    12901 aatagtattt gtttcatttc ttatcaccat tttagcataa aaggatataa gctaaaattg
    12961 attttttaat ctcccccatt ttaagtaaaa ccatgcacca ttttatcact acaaaattaa
    13021 gagtactact tactgatatt tcttgatttt gttcgtctat acgtatataa tatatgctga
    13081 taaattatga ggaaaggatg attgaaatat ggaatatatg tcttgcccag aagtagcaaa
    13141 aaaatggggc atttctgaaa gaagagtaca aaaactatgt gaagaaagtc gcataccagg
    13201 agtttcaaaa ctcgggtata tgtggctgat accaaagaac gcagaaaagc ctgttgatgg
    13261 tagagcgaaa agaagaaagg aagtacaata tgaatagcgt tttgattata gatgatgaca
    13321 aagaactttg tgctcttatg aaaaaatgtg ttgagcaaga aaatttatct gctattactg
    13381 cacattgtgg tgcagaaggt ctacatttgc tcgataaaaa tagacacaca ctctctcttg
    13441 taattttaga cataatgatg ccagatatag acggttttca agttctgaaa aaaattagag
    13501 aaacaagtaa tattccagta attatgctta ctgctaaaaa tgacgaggat gataaagttt
    13561 ccggactacg tttaggtgct gacgattacc taacaaaacc gttcagcata aacgaactta
    13621 tggctcgtgt caattctctt attcgtcggt atactatgtt aaaccctgct tttataacag
    13681 acacagattg cataataata aatggcatga ttattgacaa gacaaaccga attgtttcta
    13741 tcaataatat tcctgttcaa cttacaagca aagaatttga tttacttgct tttctggctt
    13801 ctaataaggg acgggtattt accaaaaaac agatttatac acaagtatgg gaagaagaat
    13861 atgcttttga cgatagtaac atcatgtctt tcatcagtaa attgagaaaa aagattgaac
    13921 cagaccccaa ccatccattt tatattttga cagttcgtgg tgtcggttat cgttttaata
    13981 aggaggctta acatgaatac aagtgtctat ctacttttga tattggctct tagcctgatt
    14041 attatagcgt accttgtttc tgtcctgcta cgtgtccgaa tacaacttac acttataaaa
    14101 gatgctttag aggatataaa aaatggaaac cttaatcgcc gtattttaac agatgaaaac
    14161 gacattacaa aacaaatttg ctatgatatt aacgaaatag caatcaatag ccaatttcaa
    14221 cttattcagc aaaaacaatc agaacaagcc tacaaacagc taatgacaag tctctcccat
    14281 gatgtaaaaa ctccacttgc ttctttggtc ggttatttgg aagcagtaga aagtaaaatt
    14341 gttgtaggag aggaaaaaga cgagtatatc catgttgcgt ctaataaggc tcattactta
    14401 aagaattttg tagaaaatct atttgaatgg gtaaaattgg attctaagga acaagttttt
    14461 cattttgata tttttgactt gaatgaatta tcccgtaata ttatatctga ttggatacct
    14521 gttttagaaa gcagtcattt tgaatatgaa tttgatatac cagaaataga gtacttctta
    14581 cgtatagacg caaatgcata tacccgtatc attaataacc tattacaaaa tattattacc
    14641 catagtagtg gcaacaaaat gacattgaga atttttgaaa acaaagagca agctcaaatt
    14701 ataataacag ataatgggaa aggtatatct tcagacaatc tcccacatat ttttgaaagg
    14761 ttgtaccaat gcgaccattc acgagcttca aagggaaatg gtttaggctt ggcgattgca
    14821 aaggaactta ttaacgctca taagggaaat attaccgcca acagcactcc cggtatggga
    14881 acagaattta caatattgct tcctaaagcc ctgtaaaagt acgaggcttt cttactctaa
    14941 aacaagataa aagcaagatt atggcaaggt tttggcaagg ttcatgtttt ataattctaa
    15001 ttgtagttga ttttgacgtt gtatcaacta tcttttgtag aaacaaattt attaaaagtg
    15061 aggaaattat atatgaatga ttttgtgatt gaaacaaagc aacttacaaa aatttatggg
    15121 aatcaaacag tagttgacaa agtcaatctc catgtaaaaa aaggccgaat atatggtctg
    15181 ttaggacgca acggagctgg gaaaaccaca attatgaaaa tgattttagg attgacttca
    15241 atcacgtatg gcgaggtaga tgtatttgga gagaatatta agggatgtga aaaacgagta
    15301 tatccccgta tcggtgcaat tatcgaaacg ccaggatttt atcctaatct gacaggaacg
    15361 gagaacttgg agatttttgc aaaacttcgt ggtacagccg cccctaattc tgtaaaaaga
    15421 gcattggaga ttgtaggctt accttataaa aataaaaagc tgtttagtaa atattcactt
    15481 ggcatgaaac agcgacttgg tattgccaac gctatcctgc atgacccgga actgttagtt
    15541 ttagatgagc caaccaatgg acttgaccct attggtattg cggagatgag aaagtttatt
    15601 agggacttga gcgttgagca tggaaagaca attcttatct ccagtcatat tctttcagaa
    15661 atctcattgt tggctgatga tattggaatt atcgaccacg gtgttctttt ggaagaaagt
    15721 agtatgagtg aactagaaaa gaaaaacagt aaatatatcc tgctacaagt ttcagatatt
    15781 ccaaagacat cacttatttt agagcgtcaa tttaacacga aggattattc cgttcaagat
    15841 gaccacactt tacgccttta taacactact ctggatatgg gtgaaatcaa taaagcactt
    15901 gtaatgcaga atgtaactgt tattagttct caactttgta atgatacttt agaggactac
    15961 tttaaaaaaa tcacaggagg agagggcatt gcttaaactt attcaaacag aatttcttaa
    16021 actgcgtcgt aggaaactta tttggctgat gttgttgtct gctctcatta tgccgttttt
    16081 cgcactacta tacttcaatt attttggtaa aacaggtgtt gagccaacaa agttttataa
    16141 atggtctgca tttggttata cactttggat tattttgccc gttgttttgg gtatattttg
    16201 caccatgctt atacacgaag aaaaccagta tgatatgctc aaacagcttt ggattgtacc
    16261 tattagtaaa atgggatact tttttagtaa atttttcgtt gttttgattt actctatttg
    16321 ttttatgctg attacagccg ttgcttctgt gttatttagt gtactttctg gatatgtcgt
    16381 atttagctgg gcaagcattt tatatttgtt gaaaaaatgt ttagaaattg gtgtaatcac
    16441 tgcttttgta atgttgccga ttttggcaat cgctacatct caaaaaggat acattctccc
    16501 aatttgtatc acattggtat atgccttttc cggttttgtt ataatgacaa taaatatgta
    16561 tttacaccca ttatccagta tgggagttat tatcatgaga aataaagaca ttccaggtct
    16621 tatattttca caggaaatta atgtcccatt agcatttctt tgtatttgta tttggagtat
    16681 tgtatcagta ttgtttgcaa gcattgcact aggaagaaga aagtgaggtg tagataatgt
    16741 ggaaattact ttgggctgaa tgtcaaaaaa ttcgtcgttc aaaaattgtt tggattacat
    16801 tttttgcatc cattatgata gcagtcatag tttttgcagg agggcaggat gtatataatg
    16861 acccagattt acattatggc ttaaagagcg tttatgacgg ttcacggtat atagacaatg
    16921 caggttggta tatggacgcg gtacaacctt ggtcaacttt ttttgttctt cctgctgtca
    16981 ttgccctatt aggaagctat attatttgtc gtgaagaaga ggaggacact attaaatccc
    17041 tgcgtcttat tcccataaat gaaacaaaat taaccatagc aaaaatgatt attactttta
    17101 tattcagtat acttctttat ttgttactct ttactataac atttttgact gaatccgttt
    17161 tacatttttc cgatttatcg acaaaactgg ttttaagttg catgaaagag tattttcttg
    17221 atggcatagg tgtattcttt gcaatttctc ccattattgc cttggtatcg cgcatgaaaa
    17281 aaggatattg gcttgcatta gtattcacag aagtttattc tattgctgga ctatttgcgg
    17341 gtatgtcaaa tgtcttacaa accttttttc ctattgcagc aatatttaat atttctggat
    17401 actatataac tactggagaa aaaattatgg ggagtatcat aagtttatta ctttgtgcct
    17461 gcctttctgc atttatactg aaagggctaa aacacaatga aaaaaattga aataggtata
    17521 tcaaaatgaa aaagataaaa aaactgatta ttctaattgt attcttgttt ctttttggca
    17581 tgttacactg ttatactgaa tggaaattca acaatgaccc acctaaaggg gtaatagaaa
    17641 gaataaatga gtggactgat aagattggac tggattagta acttatgtta attatagtga
    17701 atgtaatatt agcaagttat tgtttcgtat tttaatacta taactttaaa tatttggttt
    17761 taatcttccg aacgaatata aaaaaactgt cacagaggca acatattttt gaatatttat
    17821 aaagtggtag ttttgatttt caaagctgcc gccttttctt ctatataagc aaacttatcc
    17881 tcaacaaagt gtaaaacatg gtacatttta agaaaaaagt accacagaca caagcaataa
    17941 aagaaacgcc gagaacctac aaaaacgtag tgcttatgca gatttacgac gccttctatt
    18001 ttctcaaccg tcttaatgac cttgtacaaa agaaattttt ctaatatttt ttataatctt
    18061 atcttgtgat gttttttatg tataaatgac ttaataacaa atccaataaa aattatggat
    18121 attggtataa ttagaacgag gaatttataa tcattataat gtgacattat tcttacccaa
    18181 ctctcagaaa aaatatatcc tatactgatt aatgaagtat tccatatggt tattcccacg
    18241 atagatgata ttacaaaagt tataatattt tgacctgcta tacctgctat aaaagatatg
    18301 tatgttctac ataaagggat taatctacat acacaaacag aaatagaaga gtatttatta
    18361 aaataatctt gagatgcaaa tattcctttc tttgcctttg gacatatctc tattatttta
    18421 tttatgatag atttacctcc ccaatttccc actgtataac aaaatatcga accaagtata
    18481 cctgcaatta tacttaaaat taaaattgtc agaaatccaa aatgatttct tgcagcaatt
    18541 gctccacata aaggtaaaac aacttcgcta ggtaatggaa aacaagcata ctcaatcata
    18601 attaatacaa atacagagat taatccatat tgtaaaataa aactattgat tacagacact
    18661 tacttatcta tctcctttct tatttaaagg ttttaagggt acatattcta aggattctca
    18721 ccctatattt aaagaatcaa tctctttaaa ctattatatg tatttacaca aagcatttat
    18781 aacatacttt aaaaatagta ccttaaaata agaaacttaa taaat
//
LOCUS       DAEPOZ010000051        18571 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.051, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000051 DAEPOZ010000000
VERSION     DAEPOZ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18571
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.051"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(152..718)
                     /locus_tag="KR440_001413"
     CDS             complement(152..718)
                     /locus_tag="KR440_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBH0698470.1"
                     /translation="MDTKKVTFSSICIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            884..1405
                     /locus_tag="KR440_001414"
     CDS             884..1405
                     /locus_tag="KR440_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBH0698471.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAINLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFNEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKINDIQMVDGKGIFIEELEKLLKEYNLE
                     IPIVGFDELREDK"
     gene            2196..4274
                     /locus_tag="KR440_001415"
     CDS             2196..4274
                     /locus_tag="KR440_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBH0698472.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
     gene            4274..5053
                     /locus_tag="KR440_001416"
     CDS             4274..5053
                     /locus_tag="KR440_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0698473.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            5046..5855
                     /locus_tag="KR440_001417"
     CDS             5046..5855
                     /locus_tag="KR440_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBH0698474.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            5875..6357
                     /locus_tag="KR440_001418"
     CDS             5875..6357
                     /locus_tag="KR440_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0698475.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            6378..6782
                     /locus_tag="KR440_001419"
     CDS             6378..6782
                     /locus_tag="KR440_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0698476.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANDDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNASLLSDDE"
     gene            complement(6938..8098)
                     /locus_tag="KR440_001420"
     CDS             complement(6938..8098)
                     /locus_tag="KR440_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBH0698477.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNQNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            8306..9856
                     /gene="xylB"
                     /locus_tag="KR440_001421"
     CDS             8306..9856
                     /gene="xylB"
                     /locus_tag="KR440_001421"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBH0698478.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNIIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYINTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSQVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYKIYHRLYKRIYKSLKDDFKLLDE
                     VVNTKINN"
     gene            9952..11289
                     /gene="xylA"
                     /locus_tag="KR440_001422"
     CDS             9952..11289
                     /gene="xylA"
                     /locus_tag="KR440_001422"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HBH0698479.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNVTDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
     gene            11811..13010
                     /locus_tag="KR440_001423"
     CDS             11811..13010
                     /locus_tag="KR440_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HBH0698480.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYINTLEKHKALIIDIRGNLGGSDRYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKKYTNARNIKLNPVENLPASVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKETKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
     gene            13177..13962
                     /locus_tag="KR440_001424"
     CDS             13177..13962
                     /locus_tag="KR440_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH0698481.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            14131..15696
                     /locus_tag="KR440_001425"
     CDS             14131..15696
                     /locus_tag="KR440_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH0698482.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFLFTFLIKKFDMKTPGREEDNEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKVVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            15710..17041
                     /locus_tag="KR440_001426"
     CDS             15710..17041
                     /locus_tag="KR440_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBH0698483.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARILGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMIEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            17055..17543
                     /locus_tag="KR440_001427"
     CDS             17055..17543
                     /locus_tag="KR440_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698484.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            17557..18024
                     /locus_tag="KR440_001428"
     CDS             17557..18024
                     /locus_tag="KR440_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0698485.1"
                     /translation="MFFKRKKEASTINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            18027..>18571
                     /locus_tag="KR440_001429"
     CDS             18027..>18571
                     /locus_tag="KR440_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0698486.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLP"
BASE COUNT         7104 a         2043 c         3283 g         6141 t
ORIGIN      
        1 atatagattt atagctcaaa tttatatcaa gaggttacgt ttaattaaaa taatacaaat
       61 actatctaat taaattatta ataaaaatgt tatataaaaa tgagtatata gcattttaaa
      121 gagtaataaa tcttacataa taaatgattt attaatatga actagaagta tttttactat
      181 attgttttat aaaaccttta ggcattttac ttataaaata taccaccata atacaagaca
      241 aaggtttgtc tactatatta ctaagaatcc ttggaaagaa cgcagcagta aatatcttct
      301 gaccagactg aactaaaaaa ccaaccaata aatcagcacc acttccactt aaaccaccaa
      361 acaataaaac tgaaatagga gtaccaatta aaggacaaac aatagataaa attactcctg
      421 taataatagc ttctttataa ccaaaacctt ttttcttaga aatatatcca acaataagac
      481 caagaacaat atttacaaga gcaaaaggta tatttgtagg acctgatata acacctaaaa
      541 ctaagtttgt acacccacca ataagagcac cccaaaaagg acctaatacg acagccccaa
      601 gtacagtacc aactgtatct aaaaataata atggtatatt tatcatagat ataaccatac
      661 ctaagacaat attaataact atgcatattg aagaaaatgt gactttttta gtatccaatt
      721 ataatacctc ctaaaaaaat atatacattt atcatagtat agattataaa agattactaa
      781 ttgtatattt ctatatagaa tattggaaat atagaacaaa taaataatat aattatcaaa
      841 atatgtagta tcattaaaat taagaataac taaggagatt attatgaaga gaaatagaga
      901 gattgtagta gtatgtcatt gtatattaaa ttgtaattct aaagtagagg gattgtcaga
      961 atttaaggga gcacaaaata tagcgataaa tttaataaat aaagggtatg gaataattca
     1021 actaccatgc ccagaaatga ttatgtatgg aataaatagg tggggacatg taaaagaaca
     1081 gtttgacaat ttgtttttta gagaacaatg taggcaaatg ttaatacctc atgtaaggca
     1141 gtttaatgaa tatttaaaaa atgattatga tataaaaagt atcattgcaa tagatggtag
     1201 tccaagttgt ggttataata agacatgtag ttctaaacgt tggtttggtg agctaagtgg
     1261 ttgcaatgaa ttgatggata aaataaatga tatacaaatg gtggatggga aaggtatttt
     1321 tatagaagag ctagaaaagc tacttaaaga atataattta gagataccta tagttggatt
     1381 tgacgagctt agagaagata agtaaagata taaatattta ttgtctcatt atgaaacacc
     1441 attatatttg gcagaaaaat atgactgatg gacagtacct gaatcatttt taagtggagt
     1501 aattagtact tataaagatg tatctactag ctgaaagaaa atgtagagag gtaataaatg
     1561 aattgataat atatataaag gagtgtctaa aagtgaactt tttagtttat gagacactct
     1621 ttttttatat taaatcaagt atattaaatc aaatatattt taatcatttt agcaatgaaa
     1681 aataaattta tctatatttt gagacaacat cttttttagt tttatttatt tgttgtcgtt
     1741 tttgacttgg tatacaatta tatattttct taaataattt cataaattat ttataattat
     1801 aaaatccatt gttttgaaca atatctaata tattattatc tgtatttata aggtcactat
     1861 acatattttc aagtccaata ttatctacat aataggcaaa ttttattcct gtatgtgttt
     1921 aaaaaaaact agatgtatat aggtatcatc acagatatta aaagtataaa taaaagatat
     1981 taagaacgac tttcaagcaa catattagaa aattttacta aaatccggaa agcgattgac
     2041 aaacaaagtc aaataaagta tattattggt aagttagttt aatgtacaaa tgaattatat
     2101 ataaaaccta atttataaat aacgatacta aacgacatta tttaacttaa gctacacaaa
     2161 gatagaatat tataccattg atttaggagg tagccgtgat aaaaatatct gaagactata
     2221 ttgaaaaaag atttgacaat gaactattgc gaatagaagc atggggaaaa aactctttac
     2281 gtattaggtc gtttgtagac caaaattttg ttgatgaaaa ctatgctctt aacgaaaaac
     2341 ctaagttaaa taaagatgat attacaatta ataaaaatga agatggaagt gcaatcatta
     2401 aaaatggaaa gataaaagct gtcttagacc atagagatag aattactttt tataatgata
     2461 aaaatgaaat attattgaaa gaatatattc gattaagagc tgtaaaacat gatgatggtg
     2521 gagaagatgt agggacaatt gagattacca aagactttaa ctctactcta aagttaaaat
     2581 caagagaata tcgaccaagc tatagtggag aatttgaagt aactactaga tttgaatctg
     2641 agccttctga aaagattttt ggaatgggac aatatcaaca taaattttta gatttaaaga
     2701 atactgtttt ggagttagcc caaagaaatt ctcaaatatc agttcctttt tatatttcta
     2761 gtctaggtta tggactttta tggaataatc cgggaattgg aaaagtatct tttgctaaaa
     2821 atatgacaga atggaagatg ttttctacaa attttataga ttattggatt acttgtggag
     2881 aaagcccaaa agagttaaat aaaaattatt ctcaagttac tggaactgtt cctatgatgc
     2941 ctgaaaacct tttaggacta tggcaaagta aactaagata tagaacttca gaagaagttt
     3001 tagatgttgt gaaggaatat tctaaaagag gaattaagct ttcatcaatt gcaattgact
     3061 attttcattg gcctaagcaa ggtgaatata aatttgactt agattattgg aaaaatccta
     3121 aagaattagt taaaaaattg aaagaagagt attcagtaga accaatagta tctgtatggc
     3181 ctacagttca aagtgatgca gaaaactaca atgattattt agaaaatggc tatttagtca
     3241 atgttaatcg tggtgttaga atgacaatgc aaattcaagg aaatacagtc tttgtagata
     3301 tgacaaatga aaatgcaaga gaatatgttt gggatagaat agataaaaat tataaacaac
     3361 ttgggattga ctattattgg ttagatgtag ctgaacctgg ctattctgtg tatgattttg
     3421 ataactatag atataaaaaa ggaaatgttt taagttgtgg taatatttat cctattgatt
     3481 atttaaaaat gatttatgat ggtttacata atgacataga gtctgttgta actcttgtaa
     3541 gaggagcttg ggcaggagct caaaagtatg gagctttagt atggtctggt gacattgatt
     3601 ctagttttga ggcttttaat aatcaagtaa atacagggtt aaatatggga ctggcaggta
     3661 taccatggtg gactactgat attggaggtt tccatggagg aaatcctaaa gacccagaat
     3721 ttagagaact tatggtaaga tggttccaat atgctacttt ttcaccaatt ttgagaatgc
     3781 atggagatag attgcctcat agtaaaccat tatcaaataa aggtggaggc tcaatggtaa
     3841 ctggagctcc taatgaaata tggtcttatg gtgaagaagt tgaagtaatt cttacaaaat
     3901 tcataaaaat tagagagagc ttaaaaactt atttaaaaaa actgatgaaa gaagcgcatg
     3961 aagatggaac accagttatg agaactttat tttatgaatt tcctgaagat gataaaactt
     4021 gggaggtgga taatacatat atgcttggag atgaaatttt ggtagctcca ataatgaatt
     4081 ataaagatag aagtagaaaa gtatatttac caaaaggaca tacatgggaa aatatattta
     4141 gtggagttag ttatgaaggt gggaaaactt atgaagttga atgcccgctg gaagaaattc
     4201 ctattttcct aaagcaagat tccagttata attttaaaga actaaaaaat attttgggag
     4261 aggtgaaatc ataatggagc cacaattatg gcaattatta ttaatcgtac tatatggatt
     4321 ttttataaac tatgaaaaaa attcaacaat gtttggaaca tatcaaccag taacagcagg
     4381 ttttattact ggtcttatct taggagatat aaatacaggg ttatatattg gaggtacatt
     4441 acagctatta tcattaggta ttagtaactt tggtggagca tcaatacctg attatcaaac
     4501 agcaagtata gtagccacat ttataactat tacgactaag caagaagcat ctgtaggaat
     4561 ctctataggt attccagttg ctttattgat ggtacaatta gatgttctta gaaatactat
     4621 tggtatatgg ctagttcata aagcagaaga tggagctaaa aaaggaaatt ataaaaatat
     4681 aacctatatg caaatgttag gagtactatt aacagcagct acaacaggta ttccagttgc
     4741 tctatctgta atatttggac cttctttaat taataccatt ttgaaatata cacctgaatg
     4801 gttaacaggt ggtttaacag tagctggagg actacttcca gcagtaggga taggattatt
     4861 acttagatat cttcctgcta aggagtattt tagttactta gtaattggtt ttgttttagc
     4921 agtatatatg aaagtaccat tattaggtgt agctttaatt ggtggagcaa ttgcacttat
     4981 aatttacaaa aagaatttgg aaaatcaaga acaacaatat actgtagtag gaggtatgga
     5041 cgaagatgag tgataaggta tcattaaata aatcaaatga aaaggtgaaa aaaagtgtct
     5101 tatggcgctg gttttttact agttctgttt cttcaaatta tgagaagatg caggccttgg
     5161 catactgtta tgcagtacta ccttttttaa aagtagtata caaagataat ccagaaaagt
     5221 tacaaagtgc tgtattaaat catttacaat tttttaacac taatccttgg gttgcccctt
     5281 atattttggg tattaatgta gcaatggagg agttatcaag tgaagagact gaagaggctg
     5341 ttgcttcaat taaaacaggc ttgatgggac cagtagcagg tttaggagat agtttatttg
     5401 tcgttattcc gtggacaatt tttggagcta tagcagctaa tatggcgata gatggaagtc
     5461 catttggaat cttactgtgg attgtagtga gtgtagcatt gaaattatta agtataccac
     5521 tatttaatgc tggctataca tcaggtacta agctaatatc ttcaattgaa aaaagtttaa
     5581 agattttaac tgagagtaca tctattttag gtttaatggt tgtaggtgca ttaatacctt
     5641 cagttgtaaa agctaatgtt gctttgaact ttcaacaagg tgattttaca atgaaaggtc
     5701 aagaaatatt agaccaaatt atgcctgggt tagttcctgc aattttagtt gtgattgttt
     5761 attgggctct taaaaagaat ataaaaccta tatacttaat tttaggagta atggttgttt
     5821 caataatttt atatgcatta ggtattttaa aataaggaaa ggatgatatt agatatggaa
     5881 atgaaaggta ttaaaaatat tcgtatagat gaccgtttaa tccatggtca agttgcaact
     5941 atgtggagta ataaattggg tgttacaaga ttaatggtag taaatgatgc agtagctaac
     6001 aattctgttc aaaaacaagt tttaagaatg gctacacctg caggtatagc gtcatctata
     6061 attacagaag agactgctat aaaaaatatt actgcaggaa agtatgaagg acaaaatgta
     6121 ttattaattg ttaagtctcc tgtggacctt attccatttg tagaggcagg atttaaaata
     6181 cataatataa atgtaggaaa tatgtcaagt agaaaagata caacagttct aagacctaat
     6241 atcagtgtta ctgaagaaga gaaagaagca tttaaaagac ttttagatga aggaattgaa
     6301 attacaacta taatgacgcc tgatgataag aaaacttatc ttgtagacat cttatagatt
     6361 taaagaggtg tattaaagtg aatagtatta ttttgatgag tcatggcaat atggctgaag
     6421 aagtcttaaa ttctgcaaaa atgataatag gggattctat agattatcct actgtaaata
     6481 tgaaacaaga tgatggaata gaaggaacaa tagaaaaatt aaaaaaagct ttagaagtgt
     6541 acaaggaaag ttcagaaata attataatgg cagatttact aggtggaact ccatgcaatg
     6601 ctgctttatt agtagccaat gatgacagtc gaattaaaat tatttcaggg ctaaatttag
     6661 gtatggttat tgaatcagct tttttagata caaaggaatt ggcagcaaaa ttagagaaaa
     6721 taggtaaaga taatatttct atggttgaac aaaatgcaag tttattatct gatgatgagt
     6781 aggaagatta tattaatatt taaaacaaaa tacttgtgtt aatacttaat tgatatacaa
     6841 tttttaaaaa atatagctaa tcttgcataa actatataat aaatctaatt ttagaatgta
     6901 taatttaaag ggttatggct gtatatagtc ataaccctta tatttcatta attaaagtta
     6961 aattttgtat attcaaaaag ctcttgatat ttacggcaga gccaccatat agagatgcct
     7021 cagaccctaa tgatgaaatt tctatattta ttcctttgtt aaaagagcta actaacatat
     7081 cttttgtaat ctgtaatatc tcagggatat cactaattat ttggctattt aaatatatta
     7141 tttcaggagc ataagttgta atagcattat ttatagctat tgttaagtaa ctacaaaact
     7201 catgaataac ttttttggca ttctggttat cttcataata tagttgcttt actatatcag
     7261 aatctatttt aggaatattt tctaaagaag aaagctgttc aaataccttt ttttctgaac
     7321 aatactgttc taaacagcca cgatttccac aaggacatag tttaccatta ggcatgatga
     7381 tagtgtgacc aatttctcca ctcataccat ttctgccgct atataattta ttatttatta
     7441 taatgcctga accaaatcca ctgtgaatac tgagactaag tagtgagtta tgtacagttg
     7501 aaaaagtatt ctcagctaaa gctgttaagt ttgcttcatt ttctatatga ataggaaagt
     7561 catacttttt gctaaggatg ctatataagt caatctcatt aagattataa tatggagtaa
     7621 acaatacttt attttcacaa gtaatcccat gtatagccaa agtaagacca attaccttat
     7681 aaggagtgtc tattttggat atgttataac tatttataat ttcatctatc aattgtataa
     7741 cattgtcttt acttacttgt atatctgtta atttcttaga atttataata gttccatcta
     7801 agtaagatag agaagaaaat atatagtcgt atccaatgtc catacttaaa gaaatccctg
     7861 cacatttatt aaatactaat aatatgggtt ttcttccgcc actatgagta ctatttccaa
     7921 ttcctatttc atgaactaga gattcatcaa taagtttttt ggtaatagcg gatatagttg
     7981 ctttattcaa tcctatagta gaagctatac ttgccctaga aataggacca ttttttataa
     8041 tttgttcaag taccaatctt tcattcattt ctcgaatagt gtatttatca gtaaccaatt
     8101 tgatattcct ccttaaaata atattgtaat actttttaca caaaaataaa aggttatttt
     8161 gcattgacaa agataattaa atattttatt attagttcat aagttagttt aatatactaa
     8221 caaaaataaa gcaagtaaaa tatacctaaa atataaaaaa attaggatag gaaaacgata
     8281 gttatgaagt ggcattcaag gagggatgaa gttggagtat gtattaggtg tggatattgg
     8341 tacatcaggt actaaaacag ttttatttga taaactagga aatattataa aaagttgtac
     8401 atatgaatat cctcttatac aagagaaatc aggttgggca gagcaggatg caaatgattg
     8461 gtggaaagct gtagtcgaat ctataagaga agtagttcaa agttcaaata taagcagtga
     8521 atgtataaaa ggaattggat tatcagggca gatgcatggt ctagttatgc tggacaatga
     8581 aggtaaaaca ttgagaaact ctataatatg gtgtgaccaa cgaacagtaa aagagtgtga
     8641 ggagataact gatttagtag gtgaagaaag actgattgaa ataactgcta atccagcgct
     8701 tacaggattt acagcatcaa agatattatg ggttagaaac aatgaaccag atatatatat
     8761 aaatactaat aaaatacttc ttccaaaaga ttatataaga tacaaattga caggtgaata
     8821 tgctacagag gtatcagatg caagtggtat gcagttgctc gatataagaa aaagagattg
     8881 gagcgatgaa gtactagaaa agttaaatat agataaaaat cttttaggta aagtttatga
     8941 atcacaagag attacaggtt atgtgactag agatgtagca agtttgacag ggcttaaaga
     9001 aggaacaata gtggtaggag gagctggaga ccaagcagca ggagctatag gaaatggaat
     9061 agttaaagat ggagtagttt catctactat aggtacatca ggtgtagtat ttgcttatac
     9121 gaaagagcca aaaattgata aagaaggtag agttcatact ttctgtcatg caataccaaa
     9181 tacatggcat gttatgggag ttactcaagg agctggattg tcattaaaat ggtttaaaga
     9241 taatttctgt caaagtgaag tggaagtttc aaattcttta ggtgaagatg tatatgagat
     9301 tataaataat caagtaagtc aagtaccaac aggatgtaat ggattgcttt acttaccata
     9361 tatgatggga gaaagaactc ctcatcttga cccttatgca agaggtgtgt tctttggatt
     9421 atctccaatc cattctaaaa aagaaatcgc aaggtcaata atggaaggtg tttcatatag
     9481 tcttaaagac tgtatggata taatagagaa tcttaatata gacgttaatg aagtaagagc
     9541 ctcaggtgga ggtggaaaaa gtaaagtatg gagacagatg caagcagata tgtttaatca
     9601 agatgtatat actataaatt caagtgaagg accagcactt ggagttgcaa ttcttgcatt
     9661 agtaggagct ggaatatatg aaaatataca aaaagcttgt gatgctataa taaaaacttc
     9721 aactaaatta gaaccaataa gtgaaaatgt agatacatac aagatatatc atagattgta
     9781 taaaagaata tacaaatctt tgaaagatga ctttaagtta ttagatgaag tagttaatac
     9841 aaaaataaat aattaaatta gcttaaaaag aaaaataaag atagaaaata gttttaaatt
     9901 aagaaaacat acgacatata ttatatgtgt aaaatattag gaggacatga aatgagtgaa
     9961 atatttaaag gaataggaca gataaaattt gaaggagtaa aatctgacaa tgaattagca
    10021 tttagatact acaatcctga acaagtggta ggtaataaga ctatgaaaga acacttaaga
    10081 tttgcaatga gctattggca tactttatgt ggagagggta atgacccatt tggagtaggc
    10141 acagtagaga gaccttggaa taatgtaaca gacccaattg agatagcaaa gattaaagtt
    10201 gatgcaggat ttgaatttat gtctaagatg ggaattgagt atttctgttt tcatgacaga
    10261 gatatagctc cagaaggaag agatttagaa gagacaaata agatattaga tgaaatagtt
    10321 gagtatataa aagttaatat ggaaaagaca ggtataaagt tattatgggg aactgccaat
    10381 atgtttggaa atccaagatt tgttcatggt gcatctacaa catgtaatgc agatgtatat
    10441 gcatatgcag cagctcaggt taaaaaagct atggaaataa ctaagtactt aggaggagaa
    10501 aattttgtat tctggggtgg aagagaaggt tatgaaactt tacttaacac taatacagaa
    10561 cttgaaatgg ataactttgc aagattctta caaatggctg tagattatgc taaggaaata
    10621 ggattcactg gccaattctt aatagagcct aagccaaaag agcctactaa acatcaatac
    10681 gattttgata ctgctactgt tttaggattt ttaagaaagt ataatctgga taaatacttc
    10741 aaaatgaata tagaagcaaa ccatgcaaca cttgcaggac atactttcca acatgaatta
    10801 aatatagcaa gaataaacaa tgttttaggt agtatagatg caaatcaagg agacctatta
    10861 ttaggttggg atacagacca atttccaaca aatatatatg atgcaacact tgctatgtat
    10921 gaagtgttaa agcaaggtgg aatagcacca ggaggattta actttgactc taaagtaaga
    10981 agagcatctt ttgaggtaga agacttattc ctagcttata ttgcaggaat ggatacattt
    11041 gcaaagggat tattaatagc tcataaatta ttagaagatg aggtatttga aaactttact
    11101 aaagaaagat atgcaagttt tagtgaaggt ataggtaagg atatagtaga aggtaaggtt
    11161 ggatttaaag agttagaatc ttatgcactg caaatgccag ttataaagaa taagtctgga
    11221 agacaagaaa tgctagaagc tatacttaac agatatatat atgaagtaga tacaatttct
    11281 aataagtaat tgtgtagtaa ttaaacttgg atgtttgaat aaaataacag gatatgttct
    11341 tgtttttatt ctaaaacgga caaagataag tatttgtaaa aattctcata atcatgataa
    11401 attaaggata tagacttaag tataattagt ttatatcctt aaaatttcta taaaaaataa
    11461 tcatttctat agttatcaat atttttaatt atcaatacct ttctcaacta ataaataaaa
    11521 atcagtaaag tatctaaatt taagtcatta taatggataa attttttagc atatcttttt
    11581 tagtatatca ctgataagaa tttactttca ggatatagca gtaaaataga tatttgaata
    11641 taattgttat tttataactt tcatgaaata attttgaaaa tacaaataaa aatttttaaa
    11701 tagaataata gtggaatata tcttcaagaa tttcttttaa ctgtttaggt gataataaaa
    11761 taatgataca atgtattaaa atataaaatc tgaaagtgga tgagaattac atgaaaaaga
    11821 aagtattatt tttagtaatt attttagcat ttataatctt attaggtaat tctacttatt
    11881 ctcaaaagaa tcagttgtct gaagaggaaa aggtggatga ctttaattat gtgtataatg
    11941 ttgtaaagac aggatatcca tatctaggtg taaataagag acttaataat attgattggt
    12001 tagcaaataa ggatgaatat acaaaacgaa taaaaaacac tgaaaatgat gaagaattta
    12061 tagaagaact gtcatctata ttatctgatt taaataataa acacacagaa gtaatagata
    12121 ataagaaaag atatgaactt tttaagaaat catactctaa taatgattgg tatgattttc
    12181 ttaatgataa aaaagttgtg gatagatatg actctatgaa tcttaaaata aaaatgccag
    12241 atgatatttt tgtaaaaaaa gaattaattt taaaggatgt tattaagggt cagattggat
    12301 atatgtattt accatcaatg tcatcaaaga atggctctat aaataatgat ttaaaaataa
    12361 ttggagatta tattaacact ttagaaaaac ataaagcttt aattattgat ataagaggta
    12421 atttaggtgg aagtgatagg tattggcagg gaatcgtatc taaactcata aaaaatgatg
    12481 tcaaaataaa tggttataga atttatagaa ataataatga aatagtaaaa aaatatacaa
    12541 atgcaagaaa tataaaatta aatcctgtag aaaatttacc agcaagtgta aaggaaaatg
    12601 cacctaaaga aattataaaa gattttaatg attttgaaga tacttcctat actataaaat
    12661 ctacagatga ttcaaaattt aagggtaata tatacttact tgttgataga aaaatatatt
    12721 catcatcaga gtcatttgca atgttttgta aagaaactaa atttgcgact ttagttggtg
    12781 aaactactgg cggagatgga gggtgtatag accctgtatt gtttaattta aaaaatagtg
    12841 gtctaatagt tagaatggca agttgtatgt atttaaataa gaatggaata tgtgatgagg
    12901 aatttaaaac tattccagaa tttaaagtaa aagattgtaa aaggactgct gattttaaag
    12961 atgataattg cataagaaaa gcactagaac ttgaaaatat taattattag ataaaagaat
    13021 aaaattaaaa ctacattttt ttaaacatca attatagcag tattttttat agtcaatttt
    13081 atgggctaaa tagtaaatat aagaatacgc tgtaatttaa tgttgaatat tggtataata
    13141 tttatgacga tttttaatta aaggggataa tagtttatga gattagaaga aatgattttt
    13201 ttaaattata ataaattgaa tgatacagat ttattaatat ggaaatatat aactgcaaat
    13261 aaaaaacatt gtagtaagat gtcaataaat gaacttgcta tgaaatgtaa tgtgtctcgt
    13321 gcaactatta caagatttgt taaaaaatta gatttaaaag gtttcagtga gtttaaagta
    13381 ttattaagct gggaatcaga aaaggttcat gtcatagaaa atcagtcatt tgatattgca
    13441 tgtaattcaa taattaagta tgttgaagat caaaaaaata aagattatga tgcattgtgt
    13501 aaacttcttt atgaatcaag aacaatatac gcttatggtt caggtgatat tcaaaatgtg
    13561 gttgcaaaac agataaagag aatgttctta tcatgtcaag aaataatata tgattttgag
    13621 ggaagaacat ttgatgattc tttttacaat attgtcagta aaaatgatgt tatgattatt
    13681 atatccctaa gtggtgagaa tgagaataca cttaatattg ctagaagatt aaaattaata
    13741 ggaactaaaa taatttcaat tacagagttt aaaaataata cattgactga tttaagtgat
    13801 gaatcaattt atatttcagc gactaatatc tcatttttac aaatgcaccc tagttataaa
    13861 ttgacaatgt tatattttat attgattgag ttattattta ttaaatatag tatttataaa
    13921 aggcatagaa tgattacaga agggattgat tgcaaattgt agtcattctt ttttttatga
    13981 gaatttgaac ataaaaatgt gcaaaaactt atttgactgt gaataagctc caaatgtacc
    14041 ctaaagattg tatatattta ttaacctgtt aaaataatag atatctaagg ccaaagataa
    14101 actaaaaata tgaggagggg agtaatttat atgatgaaga aaatacagcg ttttggaggg
    14161 gcaatgttta caccaacact attatttgct tttgccggga taatggttgg attctcaatt
    14221 gtatttcaaa atcagtcaat tatgggttca cttgctacac cagaaaatat ttggtaccaa
    14281 ttttggggtg taatatctag tggagcttgg atggtattta atcaattacc attattattt
    14341 gctatttctt taccaatagc acttgcaaaa aagcaacaag caagagcttg tatggaggct
    14401 ttagcaacgt atttgacttt taattatttt gtaggttcta tgttaagttt ttggggaaaa
    14461 agctttggag ttgatttcgc agctgaaata agtgctggaa gtggacttgt aagcattgct
    14521 ggtattaaaa cattagatac tggtatggtt ggtgctttgt taatatcagg tattgtcatt
    14581 tatattcata atagatttta tgataaagaa ctaccagatt ttattggatt atttagaggc
    14641 tcatcacttg tagttgcaat ttgtttcttt gttatgatac cagttgcatt attaacttgt
    14701 tttatttggc ctcatattca aaatgttatt agacatttgc aaacgttttt tataaatagt
    14761 ggaaatatag gagtttggtg ttatgcattc ttacaaaaga ttttaatacc aactggatta
    14821 catcattttg tatatgcacc aatttgttat gatagtgttg ttgttccagg tggtacatca
    14881 gtatattggg ctactcatat tcaggacttt caaacttcag ctaagactct aaaggaaatg
    14941 tatccaatcg gattttcatt atctggatta tcgaaggtgt ttggttcatt aggtgtattt
    15001 ggtgctttct atgtaactgc aaagccagaa aaaaagaaaa aagtattagg tttaatgatt
    15061 cctgctactc ttactgcagt attaactgga attactgagc cgttagaatt tacattttta
    15121 tttgtagcac cacttttatt cctagtacat gcatttttag atgcatgctt gcaaacgatt
    15181 tcgtttgcct taggagttgt tggtgatttt ggtggaggaa ttattaactg ggttgtatta
    15241 aattggcttc ctcttggaat gtatcactgg aaggtatatg ttgttcaagt tgttgtaggg
    15301 attatatttt catttatctg gttcttttta tttactttct taattaagaa gtttgatatg
    15361 aaaactcctg gtagagagga agataatgaa gaaacaaaac tatatactaa aaatgaatac
    15421 ttagaaacta aagatgaaaa aggaaataaa ttgagtaaag cctctcaaca agcatctgaa
    15481 tacataaaat tagttggtgg agcagaaaat gttgtagatg ttacaaactg tgctacgagg
    15541 ttgagattaa ccttgaagga tgactcaatt atttctaaag aagaagattt taaggtagta
    15601 ggtgcacatg gtttagtgca taatggaaaa gctattcaga ttattattgg gctttcagta
    15661 ccaagtgtaa gagaagaatt tgaaaattta ttataaagga gaaggaaaga tggaaagaaa
    15721 aagacagagg atactaattg ctggtggggg tagtacttat actgctggaa ttgtaacaat
    15781 gttaattgaa agtgttgcta aatttcctat tgaatcaatt aaattatatg ataataatga
    15841 tgagagacaa agaaaagttg ctgaagcatg tgcaataatt gttagagaga aaaatccaga
    15901 aattaaattt agttatacaa caaatccaga agaagcattt actgatattg attttgtaat
    15961 ggcacaaatt cgtgttggtt tatatgcttt acgtgaacaa gatgaaaaaa ttccattaaa
    16021 atatggggtt gttggtcaag aaacttgtgg ggcaggtgga attgcctatg gattaagaac
    16081 aataggtcca ataatcgaaa tgattgatta tatggaaaaa tattctccaa atgcttggat
    16141 gcttaattat tcaaatccag cagctatagt tgcagaagca tgtcgtgtat taagaccaaa
    16201 ttcaagaatt attaatattt gtgatatgcc agtatgttta gaggaaatat ttgctagaat
    16261 tttaggttta aattctcgta aagactttga tgttagatat tatggattga atcatttcgg
    16321 gtggtggaca agtattaagg acaaagaagg aaatgattta atgcctaaat tacaggaata
    16381 ttgtgctaaa aaaggatatg aggaatttac acctcaaggt caacacaaag aatctagttg
    16441 gttagaaact atgagagcag caaaggactt attagaaata gaaccgacta cattacctaa
    16501 tacttattta aaatattatt taatggctga tgaaactgtt gaacatgcta atccaaatta
    16561 tacaagagca aatgaaatta tggatagaag agaaaaggat acattcgaag aatgtgaaag
    16621 aattataaaa aatggcacag caagggatac atggtttgat gcaagtgaac attctcaatt
    16681 tattgttgaa ttagcttgtg cattagcatt taatactcag gaaagatttt tactgattgt
    16741 tcctaataat ggagctattg aaaattttgc agatgatgca atgattgaaa ttccttgcct
    16801 tgttggaaaa gatatggttg aacctatgtc tataggtaaa attccaactt tccaaaaagg
    16861 tttaatggaa caacaagttg cttctgaaaa attagctgta gaagcatgga tagaaaaaag
    16921 ttatcaaaaa ttatggcagt cgtttactat gagtaaaact gttccaagtg caaaagtagc
    16981 aaaagaaatc ttagatgaaa tgattgtggc taataaggat ttctggccaa cttttaaata
    17041 aggagaacaa aaacatgata acaaataaaa taaaaaatat aacagcagta ttaaaagatg
    17101 aaaatatatt tctttcagtg gttatggttt gttttctaat attacctgtt ttatatatag
    17161 tgtatttaga attgacatca ttatttacaa acatttcatt ttcacaactt ttaaacaatg
    17221 atgttaatgc aagtttaaat ttaatctcta actttagtgc agtatacagt gcatatgtta
    17281 tttacaatat aaaaaagaat aaagagataa aagaaaacat gattgctttc tatttagtct
    17341 tattagggca gatattccaa atttacacaa taactattgt tatcatgttt acctatattt
    17401 ataaatttgt tggaattaaa caaatcataa gcagtattaa agaactacaa tggggaaaga
    17461 gctataaggc tcttttttcc tcaattgcag tatgcttaat atcgttattt gtaatgataa
    17521 ttagaataaa gttaattatg tagaggagtg tctataatgt tctttaaaag aaaaaaagaa
    17581 gcaagtacaa ttaattcata cattgatgga aaaataatta atcttggaga aattcctgat
    17641 aaagtatttt caactaaggt aatgggagat ggatttgcta tgatagcaaa ggacaatatt
    17701 cttaaatcac cgactaaagg agtaataagt tttattgctc aaactaacca tgctatagga
    17761 attaaattgc aaaatgatgt tcaaattctt attcatattg gattggatag tgcaacaaca
    17821 aaaaaagaaa actttaaatt agaagttaaa caaggtcaag aagttaatat gggggaaaag
    17881 ttagtagaaa ttgataataa gttacttgaa aatactttat atattccttt agttattgta
    17941 gagaatccta atacggtaaa atatcatctt tcaaacttaa atcaggaagg aaatagtggt
    18001 attaaagtta tcaattttga ttgattatga ttaataaagc gattaaattt aaatttatca
    18061 tattctattt tttctcatat attatttttg cctttgcttc aacaaaattc acacctttct
    18121 tatcaaaatt aggttactca gcgtttgaaa gaggaattat attatcgagc tatgcaataa
    18181 ccaatatatt atttcaatta ttatttggag tattagctga taagtatcaa acaatgaaaa
    18241 aaattgtttt aattagttta tcagtttatg gaattgcatc agttattatg ttttctactg
    18301 aatctgcaac ttttataata tacttattac ttgttgcttt atcaggagga ttattaaata
    18361 cttgctgtag tttatatgat acatggataa taggttgtgg agataatatt aataattatt
    18421 tgtcatttat taagactttt gggtcgattg gatgggctat tggaagtatg gtagcatctt
    18481 acttgattct tagattctca tattttggat tatctgtagc gattggttta attgtagtat
    18541 tacttattat aaatatacta ttattacctg a
//
LOCUS       DAEPOZ010000052        18535 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.052, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000052 DAEPOZ010000000
VERSION     DAEPOZ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18535
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.052"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(298..720)
                     /locus_tag="KR440_001430"
     CDS             complement(298..720)
                     /locus_tag="KR440_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBH0698487.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
     gene            1198..4101
                     /locus_tag="KR440_001431"
     CDS             1198..4101
                     /locus_tag="KR440_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0698488.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEESEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILVL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     ISIKIPDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIVTLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDENLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPVKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            4134..4580
                     /locus_tag="KR440_001432"
     CDS             4134..4580
                     /locus_tag="KR440_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0698489.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGEMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            4703..5005
                     /locus_tag="KR440_001433"
     CDS             4703..5005
                     /locus_tag="KR440_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0698490.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            5186..6592
                     /locus_tag="KR440_001434"
     CDS             5186..6592
                     /locus_tag="KR440_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBH0698491.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPIITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFSDPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVISMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            6936..7994
                     /locus_tag="KR440_001435"
     CDS             6936..7994
                     /locus_tag="KR440_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBH0698492.1"
                     /translation="MGDKMRASVLYNVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYASPYPGRAWTATLDFMGKGDIIFKPMISHKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            8069..8755
                     /gene="araD"
                     /locus_tag="KR440_001436"
     CDS             8069..8755
                     /gene="araD"
                     /locus_tag="KR440_001436"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBH0698493.1"
                     /translation="MLEILKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNVVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            8773..9420
                     /locus_tag="KR440_001437"
     CDS             8773..9420
                     /locus_tag="KR440_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBH0698494.1"
                     /translation="MLDSLKGKVVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GIGREELNVEHICVLDLNGNVIEVEKGIKPSSEVLMHIEIYKIRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIAPYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFESWKYPEEIKF"
     gene            complement(9593..10960)
                     /locus_tag="KR440_001438"
     CDS             complement(9593..10960)
                     /locus_tag="KR440_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HBH0698495.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLRSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(11150..11238)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            11666..13786
                     /locus_tag="KR440_001439"
     CDS             11666..13786
                     /locus_tag="KR440_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBH0698496.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLRSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESILKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKICNKLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNVLEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKEIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRIFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            13890..15143
                     /locus_tag="KR440_001440"
     CDS             13890..15143
                     /locus_tag="KR440_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HBH0698497.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFEDIETYSKDSQKKREKEL
                     DKFINTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEIYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSQMIDYIESVYPREKFTQV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGFMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVITMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(15297..16358)
                     /gene="ytvI"
                     /locus_tag="KR440_001441"
     CDS             complement(15297..16358)
                     /gene="ytvI"
                     /locus_tag="KR440_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBH0698498.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLSLILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFLELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFIGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
     gene            16632..17372
                     /locus_tag="KR440_001442"
     CDS             16632..17372
                     /locus_tag="KR440_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HBH0698499.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFHRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            17384..>18535
                     /locus_tag="KR440_001443"
     CDS             17384..>18535
                     /locus_tag="KR440_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH0698500.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRILEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKV
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIF"
BASE COUNT         7438 a         2020 c         3097 g         5980 t
ORIGIN      
        1 tgaataaatt aatatctttt ctaaattaga agaggataat cttattagat aaagattatc
       61 ctctttattg aaagtttaag ggatattaga gattattaat ttttctctct ttttttaact
      121 aaaagcaata atacataaaa ttattattgc taatgatact cttaatgtgg tatatagtca
      181 tagtgtcaca tgtttatcaa tagttttttc attaattatt gtaacgaata gtaggactca
      241 taaaatactt agaaatatgt ttttagatag attttaaata tataatctaa cttatatcta
      301 taaattaaca ctattattct ccaattccat ttctagtttt tttataaatt tattaaattc
      361 ttctggttgc ctttttccaa acaatactct tacaagagct gaattgattt ttccaaatat
      421 attcctcttg ctctcacttt caataaaaat aagttttgta ttttctaagt caatatgtac
      481 taattcatat ctagtaatag tactttctct tttattgctt gtttttatct catatactct
      541 atttttttcg aaattagtta tttcaacctt aaatctttta ttattttttt gttgtacaaa
      601 tgttcctact gcttttttct cattaaaatt aggaaaattt agtcttgcag attttgtaaa
      661 tatattaaat atgtcttttg cagaatattt taataaaact tcagttttaa atgtattcat
      721 atcgttttcc cttctgtata taaatttaat atttataagt taatttttaa gttaaaatag
      781 tataatatgc catattaaat catgttaaaa ctatttatat agctaaaacc ttacctatat
      841 aagtatgtat actatcatat aaaaataaaa tcttaaaata gaaagggtta aaaacacagt
      901 ttattataat ttttattgtt gagtagatac gtactattat atatgattga gcaagttttt
      961 caatcataaa tttataaagt attttgcatt aaaatttaaa caaagagtta aattatttaa
     1021 gattatgaat aactaataca tttagtaaat tactaaatat acaaaatgta aatacttatc
     1081 aaaatgtata aatacttatg acatctagtt acttacaggg tttaagtaga aaaaaatatc
     1141 aaagtaagta tggcatgata gttgcttata tatttatcaa gaaaggagtt attaagcatg
     1201 gagttgagag ataaacttgt aaacatcata aatacagaaa ataaaaaaga tccattaaca
     1261 gatgttcaaa tagcaaagtt tttaagtaca actagagaga acataactaa tttgagaaaa
     1321 gaacttaata taagtaattc aaggcaaaga agatatcctt atttaaaatc agcaatttcg
     1381 gcaatattac aaaagaataa gaatatttct atatcagaaa ttacaaggga attgatgaca
     1441 gaaggcttta atatatcgag aagagttgtg gaggaacttt tacctaaaga aagtttagaa
     1501 atggatgtag tagaggaatc tgaagaagaa tcgaatgacc catttgaaac tctgataggt
     1561 aataaaggga gtcttagaaa tgctgtggag caggctaaat ctgctatatt gtatccacca
     1621 aaaggattac caacgttgat tattggggaa agtggagttg gtaagagttt attttctaga
     1681 catatgtatg agtttgcaaa gcaaaaaaag atagtaaaag aaagctccaa ttttgttgta
     1741 tttaactgtg ctgattatag tgacaatcca caattattat tatctttgtt atttggatat
     1801 aaaaaaggtg catttacagg tgctgaatat gatacaccag gtcttgtaga agaagctgat
     1861 gaaggagttt tatttttgga tgaaatacat agattgcctc caaaaggtca agaaatacta
     1921 ttttctatct tagatagggg aaagtttagg agattaggtg aaactaatgc tgaaagaaaa
     1981 gtaagtatta tgttgattgg ggcaactact gaaaatgtag agactaattt attattaaca
     2041 ttcagaagaa ggataccaat gctaataact ttgccaccat tacatgatag attactaaaa
     2101 gagaaaatag accttatata caatatcttt caacaagagt gtaatagaat aaatgcaaaa
     2161 atatttgtag ataaaaatgt gattgaaata cttgtactaa aaaagtttag tggaaatata
     2221 ggacaattac aaaatatgat tcaggttctt tgtgcaagag cttttatgaa gtttataaat
     2281 tccaaagatg aagatagtga atcgatagta gtagtagata taaatgaagt tttaaagctt
     2341 aaagattctt ttaaagatgc tgcctttcaa gagattgaat atacagagat aagaaaatat
     2401 ttgaaaaatg ctatatttat accatttaat ttagaagaat catcagtaaa aggaagtctt
     2461 ttaagtgatg aatatatatt accagaggac atatacaaaa caattgaaaa gaaatattat
     2521 gacctaaaat ccctagacat tgaagatata gatatagaga atattttatg gacttttatt
     2581 ttaaatagat ttgctgactt aaagtttaat ctcgatagta atgaagaaat tttttcaatg
     2641 agtgatttaa atagttttgt aagtgatagt ttagttaaac tggtaaaaga acttatgaat
     2701 gatatagttg aaaaaaatat gaatttagaa gtaaataaaa atatgttcaa atatctagca
     2761 atacacttag aagaagctat aaaaagaatt aagctaaatc aaaagataat aaatgtaaat
     2821 ttagaaaaaa taaagattga cttttcaaaa gaatatgaaa ttagtaaaag ttttgcaata
     2881 aaaattgagg aatcaattag tattaagatt ccagatgatg aaattggatt tataacactc
     2941 tacataaagt ctgctctaaa aaatgaagtg aaaaaagata aagtagggtt aataataatt
     3001 tctcatggac gtatagctac agagactgta aatgtagtta aagaactttt aggtgtaaag
     3061 tttcctgtgg ctatagatat gcctttagat gaaaaaccaa taaatatata caataaggca
     3121 gtagaactgt caaaaataat agaccaaggg aaaggtatat tgtttttggt ggatattggt
     3181 tcacttacaa acataggtca aatagtaaat aagagaacca atataagcac taaaactatt
     3241 gatagagtag acctcctaat ggcactagaa gcaactagaa aagtttctat gggagaggaa
     3301 gaacttgatg aaatattttt ttctttgcta aaagatagga tgggatataa ctatgaatta
     3361 gaaaaacaca tagataaatc aaatgcaatt gtaacattat gtcttactgg agaaggaact
     3421 gctaagtata taagtaaaac gttggaagaa aagtatgata acactaaatg ttatcaaatg
     3481 agtgcattgg atgaaaattt attttgtaaa attgaaaatc taaaagagac aaacaatata
     3541 ttagctattg ttgggacaat aaaccctaag ataccaggta taaattttat accctataat
     3601 aaagaacttt tcaaaaattt ggatatctat ctatcaaaat caaaacaaca agatgacaga
     3661 ccagttaaat cgtatgaaag aatgcttgat gaggatttag tgatttttga accagatata
     3721 tattttaaaa aggatttatt agaatatgta tgttcaattc ttataaataa agaatatgta
     3781 gaaaaagatt acttagattc tgttttacat agagaagaaa tgcttccaac atattctaaa
     3841 ggagctatag gtgtgcctca tggagattct tctactgtaa atagtacaag gtttgttttt
     3901 gttaaactaa agaatcctat tgattggggt gtaggtaatg taaatttcat atttatgcca
     3961 gtttttcagg ctaatgataa agagattgtt aaaaatgttc ttgagatact aaaagatgca
     4021 gaatttatga acaatgccaa taagtgtttt gataaagatg cttttagaag gattatattt
     4081 gataaattta aacatctgta gccatagcaa aaaaattgag aggagttaga gtaatgaatg
     4141 tagatttaat atcattgggt ttagaatcta atgattatga aagtgttatt gaagaattag
     4201 gaagtataat gtgtaaaaaa gaatatgtaa aagaaacata cataaatgct gtcttggaaa
     4261 gagaaaggac attaccaaca ggtctagata ttggagaaat gtgtgttgct attccacata
     4321 cagattcaaa gcatgtaaat gagtctaatg tagctgtagg gatattgaaa aatcctgtaa
     4381 agtttaattc aatgatagac ccaaaagata gacttgatgt agaacttgta tttttattag
     4441 cagtaaagaa cccagattca caagtaaaac tgttaaaaga tttaatgtca gtttttcaaa
     4501 atataaagtt attaaaaaat ataaaaaacg catcaacaaa agaagaagta gctaaattat
     4561 tggattttat agaaatttaa tgtaggagaa ttttataaat aaaaaattaa aatttcaaca
     4621 atcaaagtaa gtattatttt tttaataggt ttaatcttat ataaaataaa aatttagagt
     4681 gaaaaattag gaggaaatta ttatgaaaca atttaatgta ttatcagttt gtggttcagg
     4741 gacagttaca tcatctatgg tagcaggtaa attaaaagaa gtactaggta ataaggggat
     4801 ttctattact acaacagaag ctagaccaac agaagcacta aatttagcac aatcaggaag
     4861 atttgacttt ataacattta cgagtccgct tcaaacaggc gattatggaa taccaactat
     4921 aaatgcattt gcttgcctaa ctggtatagg ggaagaggca ttctttgaag aagttttgac
     4981 tgttattgaa ggcatagata agtaagtttt ggagacgtga atttatagta tttaaatcaa
     5041 tgatttagat actataataa gctaatatta actaaatatt tgtggaaaaa acattttata
     5101 tgttgtcata aattaaaaaa ttataaaaat agtcacttaa gtattaaatt aagcttataa
     5161 atatttataa gtaggaggga aaaagatgtt agaatttatg aaatctataa tcgatacatt
     5221 tggttctgca attatagttc caattataat attcataata gcaaagatat ttaaagtaac
     5281 tactaagaaa tcgtttcttt ctgctgtgta tgcaggagtt gctttacaag gttttacact
     5341 gattttaaat tcatttacac caataataac accagttatc aataggatgg ttgagagtac
     5401 aggagtaaac ctaccagttt ttgatgttgg ttggcaagca acatcattgg ttgcattttc
     5461 aacaagtgca ggtatgatat atttaggtct tggtatatta ttacaaacaa ttttattttt
     5521 gataaaatgg acagacgtgt ttcaaccatc agatttatgg aataactatt cttatatggt
     5581 atggggagca atggttatag gagtaacagg aaattttcct ttaggtatag catgtatggt
     5641 tttattaaac ttatatagtt tattaatatc agagttagtt gcaaagagat ggtctagtta
     5701 ctatagatat cctaattgta caataatagc tatgcataat gttgaggctt ctgtgtttgc
     5761 agtattttca gaccctatat acaataaatt gggattaaat aaaataaaat taaatccaaa
     5821 agaattagag aagaaattag ggtttttagg tgaaccaatt actttaggtc tttttttagg
     5881 tatgtttata ggtatattag gtaatatgac gagaataaat actatggaag cttggggaga
     5941 aataatgaaa gtaggtatat ctacaagtgc agtgatggcg attttcccta aagtagcaag
     6001 tatgtttgct caagcatttg cacctataac agaagcagct agaaagatta tgcaaaaagc
     6061 tggaaaccgt gagtggtaca tagcagtaaa tgatgctgtt ggatatggtg aacctgcaac
     6121 tttaatatca ggtcttatat taattccaat aatgcttgta atatctatgg tacttcctgg
     6181 taataaggta ttaccagttg tggatttgtt ggcaattcca tatatggttc aaggtttagt
     6241 tgccatacat aatggtaata taccaaaagt attagtttca ggtataattt ggtttggatt
     6301 aggattatat gtttgtacat ctacagcacc attatttaca gatatggcta caaatatagg
     6361 tgtagctata cctgcaggag caatgttaat aacaagcttt aatatactag gaaaaccttt
     6421 gatgggatta gttttctttg catttctaag tgcaaatcca atttatatag gtctttcagt
     6481 tgtaatttac tttgttctat gggcattgtt tagaaagaac aaaacaagta ttcttgatta
     6541 tttagaaaaa caagcactta aaaatgtaga agaagagcct gtagcagttt aagtttataa
     6601 aaatgaaata agaataatta aaggagttta taattgattt tatataagta tcagataata
     6661 cctgagaaat atttaaatga aatatagact tctttttatt tgataatatt tgtaattata
     6721 atgcattgta tttttatagt tgagttttct gtataacctg ctatttaaaa catactcttt
     6781 tatatttata cagtaatgtg gtttaatttt ataaaatgta atctctactg gtaaaagact
     6841 ttgtatataa ggcatttgcc tgaataaacc atttatcttt tgtatggcat agatattgct
     6901 atatagaagt atatagcatg aaaggagata tttatatggg agataaaatg agagcatcag
     6961 tattgtataa tgtaggtgat gtcaggtatg aaatggtaga tataccagaa ataacagata
     7021 cgcaagtgct tgtaaatgta aaatatgttg ggatatgtgg ttcagatttg cctaggtcta
     7081 tggtaagtgg tttgagtgga aatacaaaat atccattgat tttaggacat gaattttctg
     7141 gtgaagttgt taaaattgga gaaaaagtaa agcatattaa tgtaggagat agagtagctg
     7201 ttgcaccatt agtaccttgt ggaaaatgtg attattgtaa tgaaggtaat tttggcttgt
     7261 gtgatgatta caatataatt ggaacaagag taaatggagc gtttgcagaa tatgtaagag
     7321 ttccagaaga acatatttta aaactaccag atacattgga ttatgaaact gctgccggaa
     7381 ttgaaccagc aactatagct tatcatggga tttcaaagtc taatattaga gtaggagata
     7441 gtgtagttgt gttaggatgt ggtccaatag gtcagtttgt tatacaatgg gcaaaggtgt
     7501 ttggtgcatc taagattata gcagttgata tttttgatga aaaattagaa ctttctaaac
     7561 ttctaggagc aaactatata cttaactcaa aagaagtaaa tgtaatcaaa gaaataaaaa
     7621 aaataacaaa tggtggtgct gatgttgtaa tagagactgc tggtagccga tttacacaag
     7681 aacaatctct atttgtagca aaaaagagag gaaatatagt gtttgtaggt atatcacaca
     7741 cagaattacc acttagtgca gacgctactg agtgtatatt acgtggagaa cttaccttga
     7801 aaggttcttg gaattcatat gcaagccctt atcctggaag agcatggact gcaacattgg
     7861 attttatggg aaaaggtgac attattttta aacccatgat ttcacataaa ataggtctta
     7921 atgaagtggg tgacttttta agtaaaatga gcaagagaga aattaatttc aataaaatcc
     7981 tagtagaaat ataaaaattt aataaaaaat tctaaaaaca attaaatttt ataactatga
     8041 cattaaataa aatataggag gaagaaaagt gctagaaatt ttaaaagaag aagtattaaa
     8101 agcaaactta gaattaccta agaaaaagct aattacttat acttggggaa atgtaagtgg
     8161 gatagataga gaaaaaggat tagtggtgat aaaaccaagt ggtgtagagt atagtaatat
     8221 gactgtagaa gatatggttg tagttgattt agagggaaat gtagttgatg gcaaactaag
     8281 accctcctca gatactccga ctcatctagt tttatataaa gagtttgaag aattgggggg
     8341 aatagtacat acacattcaa gttgggcaac aatttgggct caaataggaa aatctatacc
     8401 atcttgtggt actactcatg cagattattt ttatggaagc ataccatgta ccagaaaaat
     8461 gactagagaa gaaatttgca atgaatacga aaaggaaact ggaaatgtaa tcattgaaga
     8521 atttatggga agaaatccaa ttcattgtcc aggaataatt gtaaatgacc acggaccttt
     8581 tacatgggga aaagatgcaa atgaggctgt acacaatgca gtagttttag aagaagtcgc
     8641 aaagatggct tattatacag agttaatgag tcctgataat attatggata aggttcttat
     8701 gaataagcat ttttcaagaa aacatggaaa aaatgcatac tatggtcaaa aatgattatc
     8761 tggaggtgaa ttatgctaga ctctttaaaa ggtaaagttg tggaaatagc aattaaagca
     8821 gagcattatg gtttatgtaa agaaaagact ggtaatttta gtattagaga taaggatact
     8881 ggatatatac ttataacccc atcaggtata ggaagagaag aattaaacgt tgaacatatt
     8941 tgtgtattgg atttgaatgg aaatgtaata gaagttgaga aaggtattaa gccaagtagt
     9001 gaggtattga tgcatattga aatatataaa ataagggaaa atataaatgc tatatcacat
     9061 actcattctt tatatgcaac agcattttca gtagcaaaga aaccaattat accaattgta
     9121 tatgaatcag ttaattatgg cggttatgta gatattgctc catacgaaag accaggaact
     9181 ttaaaattag ctaagagcgt ttcaaaatta ctgatagata cagatgcatg cttattagaa
     9241 agacatgggt tgacaactgt aggtaatgat ttagacgaag cattattaaa atcaagatat
     9301 gttgaagagg tagcagaaat atactatagg tctttagtat taaatcaatt taaagaactt
     9361 gaagtagtag atgttgatga gtttgaatct tggaaatacc cagaagaaat aaagttttaa
     9421 tctaaataat tttcattgaa gtggttaatt aaaatatatg tattatttat atcaattact
     9481 atgttttatt atattaatca ccgtattttg taaatatatt tattacattt tttcaaaaaa
     9541 atatagaatc cgaaagccat ataaaattga cttgggattc tatacttttt atttatgcat
     9601 ttttctttat ctgattttct ggagaattta tatgttcaaa atatttttta gtttcagata
     9661 ctattatagg tgataatgca agtaaagcta taaggttagg tattgccata agcccattta
     9721 caatatcagc aattatccaa acaacttcta actttaagaa agcacctaat aacaccatag
     9781 ccacaaatac agttctatac ccattcatgc cttttacacc aaacagaaat tcaaaacatc
     9841 tttctccata ataactccat cccaaaattg tagtaaatgc aaataatgtt aaactaactg
     9901 taagtaatat tggtccaaat gtaggaattg ctccgttaaa agctgcttgt gtcataacag
     9961 ctccatttag gtctgaacac cacactcctg aaataacaag agagaatcca gtaagagtac
    10021 agattataat agtatctata aaagtacctg tcatagatat aagaccttgt tccgctggcc
    10081 attcagtttt agcagcagct gcagctattg gagcactacc tagaccagac tcatttgaaa
    10141 atacaccacg agcaatacca tttctaattg ccagtgacat agttgctcct aaaaaaccac
    10201 cagtagcagc agtaggtgta aatgcacttt taattattaa cataaaagtt tcagggattt
    10261 tattccaaaa agatattaaa atgattccac agattacaac atatataact gccataaaag
    10321 gtactatttt agtagctaca ttagatatac ttctaagtcc tccaaataca accaaagcta
    10381 caaatactgc taatacaacc cctgtaattc tagggtctat cccgaaacta ttatttattg
    10441 catctgttat tgaatttact tgagtaaaag taccgattcc taaaagtgca actagtatac
    10501 cacttatagc gaaaaatatt gcaagaggtc tccatttttc tcccatacca tttactatgt
    10561 aatacattgg cccaccagac acttgaccat ttttatcttt agttctgtat tttattgcaa
    10621 gaacaccttc tgaatactta gtagccattc caaagaatgc tgctatccac atccaaaaaa
    10681 gtgcaccagg tccaccagct ttgatagctg tagcaacacc aacaatatta ccagtaccaa
    10741 ctgtagcagc tagagcagta cataaggctg caaaacttga aacatcacca ctacctttgt
    10801 tttcagcaga gaatattaac ttaagagctc ttggcagttt agttatttga acaactccaa
    10861 gcttaacagt aagtagtata ccagtaccaa ctaatagtac cagtaggggt ggtccccaaa
    10921 taaaagcatc aactttgttt aaaatatcaa ttagattcat aaaaacactc cctcattttt
    10981 tttgctttac ataatcaata gaatttttaa tgaagtaatt gacaggaaat aagtataatt
    11041 tattaataat ttctagtgta ataaaaaaag ctaaatctaa aaaagattca gctctggatg
    11101 tacactaatt agcaaccaaa ataaaagtat tagtaaatac tgtttttggt agataatccc
    11161 tgtccttttg cctgagagat tgagcataaa agccttgccc cttcggcgcc cataatgggt
    11221 ctctccagag ttccgtaaat tttacagtcc tacttttaaa agtacctgag agtgttactc
    11281 cttcggtgtg ctatatagca actcttctat aaaatcttaa ccggatattg atttgtcata
    11341 gcttattgta ttaataatat taatataagt ttttatgttt tgtcaactaa aaaattaaaa
    11401 aacaaagaaa aaatataaaa atgtaagcct atttacgaat tataaactta gaaagatgag
    11461 taatatacgt aaaaatgaat tttgtaatac acatacaaat gaagtgtaaa ataagtttta
    11521 aataaaaaaa taataaaatc ttaaataatt aattagatat gattaaatta tggtaatatt
    11581 atagttacga tattaagaaa agttctattt agttagattt taagcgtaat atgagattat
    11641 gtatagagaa aggatgtatt tttagatggg aaaaatatta gtgctagcag aaaaaccttc
    11701 tgttggaaga gatatagcaa aagttttagg gtcaaaaaat gaaaaaaatg gttatataga
    11761 ggggccaaag tatgtgataa cttgggcact aggtcatttg gttacacttg ctgaccccga
    11821 aagttatgga gaaagatata aatcatggag cttagaagac ctgccaatac ttcccaagca
    11881 ccttaagact gttgtaataa aaaagagtgg aaagcagttt aatacagtta aatcgcaaat
    11941 gaatagaaat gatatagatg aaatagttat agcaacagat gcaggtcgtg aaggagaact
    12001 tgtagcaagg tggataatag aaaaatcaca ggtaaaaaaa cctataaaac gtctttggat
    12061 ttcttcaagt acagacaaag caataaaaga cggatttgca aaattaagaa gtggaaaaga
    12121 atatgaaaat ttgtattact cagcaatagc acgagcagaa gctgattgga ttattggaat
    12181 aaatgcaacc agagctttga caacaaagta caatgcacag ctttcttgtg gaagagtaca
    12241 aactcctact cttgcaatgt tgcttaaaag agaagaagaa ataagaaatt tcaaacctaa
    12301 agagtattat ggattagaat taattgctac aaagggaaat tcagatataa aatttatttg
    12361 gaatgataaa aacaataatt cttcaacttt tagtaaagaa aagatagaat ccatattaaa
    12421 aaaagtaaaa ggtgtagaca taaaaatatc tgaagtaaac aagtcatata agaaaaaata
    12481 ttcacctgca ctctatgatt taacagaatt acaaagagat gcaaataaaa tgtttggatt
    12541 ttcagcaaaa gaaactttat ctataatgca aaaactgtat gaacatcata aagtacttac
    12601 ttatcctaga actgattcta gatatttaac atcagatatt gttggaacat taaaagatag
    12661 aataaaagct gtaaatatta gtgattattc aaagatttgt aataaattat taaaaagcaa
    12721 tattatagct aataaatcgt ttgttgacaa ttcaaaggta acagaccacc atgcaataat
    12781 accaacagaa cagaaggtat tcatgagtga ttttagtgac aaagagcgaa aaatatataa
    12841 tcttgtagta aaaagatttt tagcagtact atctcctcct tttgaatatg agcagacaac
    12901 tataagtgca actgtagaag gagaaatatt taaggctaag ggaaacaaag taataaattt
    12961 aggttggaaa gaaagttata aagatattga agatgaagat gagtatgaaa gtataccaaa
    13021 tgttttagaa aaagatgtac ttaaggtcaa gggattaaag ataacttcag gaaaaacaaa
    13081 tccaccacca tacttaaatg agggaacatt attgactgct atggaaaaga atggtctagg
    13141 gacagttgct acaagagcag atataataga aaaattattc aattcatttt taatggaaaa
    13201 gaaaaataaa gagatatata taacatcaaa aggaagacaa ttattagatt tagttccagt
    13261 agacttaaag acaccagaac ttacagcttc atgggaaaag aaattattgg atatttcaaa
    13321 gggaaaactt agtaaaaata cattcataaa tgacatgaaa gattattcaa aagatattgt
    13381 actagaagta aaaaatagtg ataataaatt taaacatgat aacttaacta aaaatagatg
    13441 tccagaatgt ggtaaattta tgttagaggt aaatggtaaa aaaggtaaga tgttgatttg
    13501 tgaagataga gagtgtaata ctagaaaaac tatatcacaa gtaaccaatt caagatgtcc
    13561 taactgtcat aaaaaattag aacttcgtgg agaaggtgag gggagaatat tcacttgtag
    13621 ttgcggatac agagaaaagt tgtcttcttt caacaagaga aagcaagaag aaaaacagaa
    13681 ggcatcaaaa aaagatgtta atcaatattt aaaaaatcaa aataaaaatc aagagacatt
    13741 taataatcca tttgcagaag cattagctaa gctaaaaaac aaataatttt aaagttatta
    13801 ttatacacaa ttataacttt aactgtatat tagttatatg tactgatata atatagtgta
    13861 tagtttaggc atgaaagtag ggctgttaaa tggatttaag tggcatattt aaatactatt
    13921 gtaaagagtg tgaaaataca tggaataatt cgagtgttga attatttgag gatatagaaa
    13981 cgtatagtaa agattcacaa aaaaagaggg aaaaagaatt agataaattt ataaatacaa
    14041 tatcagttca tttagagagg tatccaagtg atgctgtatt gagaaaaatg tgggtaaaaa
    14101 agggcgaggt tttcttacaa aagacattgg aaaaagaaaa tatttttaag ttagaaaaaa
    14161 tggatgtaga ggatagaaaa aaatttttag atataacaaa acagtttatt agagatgcta
    14221 gaaaatttga tgatgattta cctataggtg atattatgca agctatgagg aatgtatgga
    14281 tttcaaatgc attacaatta ttatttggta aagaaatata ttattcaaaa gctaactttg
    14341 catatagtat gttatatcca tatactgata attatttaga caatacaaat atagataaaa
    14401 atgataagat tttatttaat aactggttag aaaaaaggct cctgggagaa catattaaat
    14461 ctaaggatta tcatgaaagt aaagtatctc aaatgataga ttatattgaa agtgtatacc
    14521 ctagagaaaa gtttacacaa gtttatgaat cgttattatt aatatttaaa agtcaagtaa
    14581 atagtttaaa acaacatggt aaggaaaatc atttgtgtaa agaagattta ttatccattt
    14641 ctattgaaaa aggaggttca tccgttttag tagatggata tttaataagt ggatttatga
    14701 caaaggaaga aatagagttt tgtataggat atggattttt attacaaata tctgatgatt
    14761 tacaagatat aaaagaagat ttaaaataca accataaaac tattattaca gagatgtcaa
    14821 aagagggtac tttagataaa gttgtaaata aactaataaa ttttactatt gagttaatag
    14881 atagttttaa aattaataat aaaaataaat ctgtaataac tatgataaag aatgattgct
    14941 taatgttaat tttattttct gtagtttata atgctgaatt tttttctgta ggatatataa
    15001 aagaagtaga gaaatttatt ccatatacaa tagattattc attagagatt gaagaaaaaa
    15061 taaaagaaaa atttaaaaat atagatgttt taaataatga aaatgaatat aaagaaatga
    15121 ttgatattat ttgtgcagag tagaaaaaaa gggagtgtct tagaatagac tttttagttt
    15181 atgaggcact ctttttgtat aaaatcaaat gtattttcat cattttaaca attaaaaaga
    15241 gcttatccct gtttgagaaa gcccttttta atttaaataa aaatgtcaag gttaaactat
    15301 ttaaatgcag taataatccc attatcatgt aagtttttta tgattgtagc tgcgataggg
    15361 aatccaaata atccagctat tccaaatagt tgagctccaa taaacataca cattaatgtt
    15421 attaaaggat ataggccaat ttggtgtcct actacctttg gttctaatat ttgtctaaca
    15481 acggttataa ttatgtatag tattaaaagt cctattgcta aattaatatt tccattaatg
    15541 agtgagataa tcatccaagg tgtcaaaata cttcctgtac ccaagactgg caaaatatca
    15601 acaagtgcta tgatgacaga tatagtaaat gcattttcaa cctttaggat agtaagtcca
    15661 ataagtagtt ctaagaatgt cactgatagt agtattgcat aggcttttag tagttttaaa
    15721 agagcattaa tactgctatt ttttatattt attatcataa attgactttt ttctggaaat
    15781 tgttttaaga taaagtttac tattttttga tagtcaaaag taaagaaaaa agaagcaatt
    15841 aaagtaataa taagttttaa cataaatgca ggtgttttac ttgcaatact agcaatagca
    15901 ttgagtgcat ttgttgaaat agaagctact aaagacatca tagattgact tatattatcc
    15961 caaccaagta ttaatgatag gttaacatta ggtgtactag catctatctt tgaaaataaa
    16021 gtatttagcg ttggttgtat acttgattta tatatttcag gaagtctaaa aaatacatca
    16081 cttacacttg caaatatctt cacaccaaag ctaactaata aaaatataca caacccataa
    16141 aaaattaaaa gtattactat agaaataagt gtacgattta aatttgtatt atttgtaata
    16201 agtcttataa taggtcttaa gatggatgca atgataaaac tgattacaaa aggcattaat
    16261 aatggcaata agaattttaa tacaatatat acaatacttg ttattaaaac tacatatgta
    16321 aatttaatta taaaattctt ttgcttttga atatccataa tataaaacct ccaatgtatt
    16381 tgatattatt ctaaatatgg tcttttttac agaaaatata ctactgtata ttgattataa
    16441 actataaagc ttatgtaaag taaataaatt atgtaaaatt taggttaatt ttgtataaag
    16501 agatactttt atattttaca attaataacc aaatactaaa aaatatttta aataaagaac
    16561 ttaaaattgt gataaaatta atttatagat attaaaaaaa gtataatata tggtttttaa
    16621 gaggtgctaa gatgaaaaat tttagagatt taggtgggaa caaaactgag gatggcagaa
    16681 ctgtaaagaa gggattattt cataggtctg caaaattatc aaatctaagt gaaaatgata
    16741 taaagatttt aaaagagtta aatattaaat atatatttga ttatagaagt gatgaagaag
    16801 ctcgtaaaca tccatcaaca ataatatcaa atataaaaaa tataagaata ccagcaatga
    16861 gagagctaga agaatcagga ggaagttttg gttctattga agatatgatt gatggattgt
    16921 ttgaaaaaga tggtgcattt aatatgttaa ataatagtta ctataatttg cctataaata
    16981 atccgtcata taaaaaatta gtagaactta taagagacta ttctaattta ccaatattaa
    17041 atcattgtac agctggtaag gatagaactg gtgtggggag tgctataatt cttatgattt
    17101 taggggtatc gagagagaat ataatgaaag attatctaaa aagtaatgat tttgcagata
    17161 aagagataga aagatttata gaatataagc ctaaatttaa ggacattcca aaagaaaatt
    17221 taaaatatat atttggtgta aatgaagagt atatgaaaac tgcatttaga agaattgatg
    17281 aagaatacat tagtgtagaa gcatatttat atggagaatt taatttaaat aaagaagaaa
    17341 taagaaaatt aagaaatcaa tatctagagt aaaaggtgag tttatgagtt taaatataac
    17401 agcaagacag ataaatataa taaaaatatt gttaaattca aaagatacaa ttagtggtat
    17461 tgccttaagt caagagatag gatgttcttc aaaaactata caaaatgaaa taaaggatat
    17521 taataaacaa cttagaaatg gtaaaatttt gtcaataaga ggagttggtt ataaactaga
    17581 aggaagtttt gatgaggtaa atctaaatag caatctctat gatgatgtag atagagtaga
    17641 atatataatc agaaaaatat taaccttaag taatgaagaa aaaaatacta taaaacttga
    17701 agatttagcc gattctatgt atgtaagtgt ttcaacagta aaaaatgatt taaaagaagt
    17761 aaaaagaata ttggaaaagt attcaatatc agttaaatct aagcataaac aaggtatatg
    17821 tgttttggaa agtgaagata agatattaaa ttgtatagtt gacttaagca ataagagaga
    17881 taatcaattg agtttaaatg attttttaaa tgacgatata aaggaaagaa aactattaat
    17941 aaaaaaatta ttattagaca tattaaatga aaatggatta gtattaactg atatagaatt
    18001 taagagtgtg ttaaataata tattagttat gttaagcaga cataagtata atgaaaaaga
    18061 atttatagag aattatatag aaaattatag gagcaaaaga agctcaattt tagaaaatga
    18121 acaaaacaaa gaaattataa taaattcaat aaaaacattt tgtaaaaatt taaaattagc
    18181 tacatcaata gatataagta atgataaggt atttgaagag tttttatata agcatattag
    18241 tagtttatgt aaaaaaataa agttaggtat aaatcaatca cctataatgg ctcaagatat
    18301 aaaaataaaa tatccatttg cttttgaact agctaatata gctaaaaaga caatagaaaa
    18361 agatttgaag atagaaatta atgaagatga agttgcaaac atagctctac atataggagg
    18421 agcagttgaa agagcatctt ataataataa agataaggtt ttcaaaacta taatagtttg
    18481 tacttcaggc ataggcacat caatgttaat aaaagctaaa ttagagaata tattt
//
LOCUS       DAEPOZ010000053        18516 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.053, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000053 DAEPOZ010000000
VERSION     DAEPOZ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18516
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.053"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(209..1516)
                     /locus_tag="KR440_001444"
     CDS             complement(209..1516)
                     /locus_tag="KR440_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBH0698501.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEEIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDEKLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(1941..3716)
                     /locus_tag="KR440_001445"
     CDS             complement(1941..3716)
                     /locus_tag="KR440_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0698502.1"
                     /translation="MMVMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDVCRECYKSGACVEFAEVCCPIICD
                     GDSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLEKMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(3713..3850)
                     /locus_tag="KR440_001446"
     CDS             complement(3713..3850)
                     /locus_tag="KR440_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698503.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(3928..4497)
                     /locus_tag="KR440_001447"
     CDS             complement(3928..4497)
                     /locus_tag="KR440_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBH0698504.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(4681..5316)
                     /locus_tag="KR440_001448"
     CDS             complement(4681..5316)
                     /locus_tag="KR440_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBH0698505.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
     gene            complement(5984..6865)
                     /locus_tag="KR440_001449"
     CDS             complement(5984..6865)
                     /locus_tag="KR440_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBH0698506.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGVKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
     gene            complement(6985..7992)
                     /locus_tag="KR440_001450"
     CDS             complement(6985..7992)
                     /locus_tag="KR440_001450"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HBH0698507.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            complement(8012..8494)
                     /locus_tag="KR440_001451"
     CDS             complement(8012..8494)
                     /locus_tag="KR440_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBH0698508.1"
                     /translation="MSFVGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSE
                     HNEWKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPAVGLTIM
                     GADGLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK
                     "
     gene            complement(8512..8979)
                     /locus_tag="KR440_001452"
     CDS             complement(8512..8979)
                     /locus_tag="KR440_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBH0698509.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            complement(8994..9752)
                     /gene="prdD"
                     /locus_tag="KR440_001453"
     CDS             complement(8994..9752)
                     /gene="prdD"
                     /locus_tag="KR440_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HBH0698510.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            complement(9832..10557)
                     /gene="prdB"
                     /locus_tag="KR440_001454"
     CDS             complement(9832..10557)
                     /gene="prdB"
                     /locus_tag="KR440_001454"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(10105..10107),aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HBH0698511.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            complement(10595..10879)
                     /locus_tag="KR440_001455"
     CDS             complement(10595..10879)
                     /locus_tag="KR440_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698512.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            complement(10960..12840)
                     /gene="prdA"
                     /locus_tag="KR440_001456"
     CDS             complement(10960..12840)
                     /gene="prdA"
                     /locus_tag="KR440_001456"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HBH0698513.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            complement(13269..15029)
                     /gene="prdR"
                     /locus_tag="KR440_001457"
     CDS             complement(13269..15029)
                     /gene="prdR"
                     /locus_tag="KR440_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HBH0698514.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            complement(15499..15661)
                     /locus_tag="KR440_001458"
                     /pseudo
     CDS             complement(15499..15661)
                     /locus_tag="KR440_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020315.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(15830..17143)
                     /gene="prdC"
                     /locus_tag="KR440_001459"
     CDS             complement(15830..17143)
                     /gene="prdC"
                     /locus_tag="KR440_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HBH0698515.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     gene            complement(17532..18296)
                     /locus_tag="KR440_001460"
     CDS             complement(17532..18296)
                     /locus_tag="KR440_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBH0698516.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKYDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKKTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
BASE COUNT         5534 a         3502 c         2021 g         7459 t
ORIGIN      
        1 aacaatgtat cttatatttt aagtagttca tatatccaca ctaaattaca tactatatat
       61 tttagacaaa aaaagaaaaa acttgcacta acaagttttt tctattatac ataaatttat
      121 acataatcat attttattag agggtactaa ttaaatttat ttatgctatt tttgttatac
      181 ctcataatac aaaacatatt ataaagtact agtcaacttt attcatatca ttcattacat
      241 ttaagattat ctcatctgtt atagacatac ctttgtcact tactctacct aagttttgta
      301 tactttgctc aacacttgaa ccaactatac catttagtgg tggtacaaaa caatcttgct
      361 tagctattat agcactttgt atagctgcag atgctgcaga agataatttt aatgcacaac
      421 cagctttcgc accgtcacat atcattccac ttaagtcagc tatcatattt tctatagctc
      481 cttctatttg cttttcagta ccattcataa gccaagagat accagcagtt gctccagtag
      541 aagcagcaac accacatcca cacacagcag ataatcttcc tgtatagttc ttaatataac
      601 cagttactag atgagatata gctaaagcct ttgctagttt ttcatcagat tgtgggaatt
      661 ttttattata agcaacgatt ggaagaattg ctgttaatcc atgatttcca cttccatttg
      721 aactcataac aggcattttt acgcctgcca ttcttgcatc tgaagctcca gcagttaaca
      781 tcattgcata atttataaca tcattaccta aaagaccttc ttctatagac tttttaattc
      841 catatccgac accaatacca gttttttgtt ttaatccata ttcagccatc tcttcattca
      901 tttttactcc atctagtaaa aattctatat ctttaaaatc aatttcttct acatttttta
      961 ttaattcttg tatagttact gtatccatta aactttcagc tttttctgcc gcatcatttg
     1021 aagctgatac ttttggttca ttgtctaata atacttctcc atctttttct aagaacgtaa
     1081 aattatcatg tttagttctt attctcactt tagcaatatg attttttcct tttaacacaa
     1141 cttctataaa tactttctct cttgtatcag caggtgttat tgatagtgga gtattatcca
     1201 tataatcctc agctatttta acttcttctt ttgttagagt ttctaacaca cttaatccat
     1261 tttcagaatg gccacctact atacctagag ctgctgcaat ttttaatcct aatctttcag
     1321 tacctggtat acctacgcac atgccatttt tgtagatact aggactaact aacatacgat
     1381 tttcaactat ttcttctcct aataattcct ttgcctttgc acatgctaga gctaaggcta
     1441 ctggttcagt acaccctact gctggtttaa cttctgcctt taacatttcc actaattctt
     1501 ttcttatatc tctcattata tataaacctc ccaattaagt ttagatttag tactcaaata
     1561 attaattaaa taatttctta tctatattta tagcaaaata catgccatat tttttatatc
     1621 tttttatatt tttagtctta tattctataa ccattggtat gactgcttct atttatgtat
     1681 tttttttatt tacatagagt atttatattc tttaaaatgg agaaacatat gacaatttcg
     1741 caaaaagaca aaaaaaagtc tcagaatgag attctatatc attttgagac ggctcaaatg
     1801 tgttattttt taacttataa aaaccatttt ttaattcata aaaaaagatt atgcaaaaca
     1861 cttatagtat aagtttaaag ctttctttaa agttatgaat ttagaaaaat ttaaatttta
     1921 tatagctttt tattatatta ctatgaaatt atattgtatt tttcaatttt tctatataat
     1981 gtagctcttg atattccaag tgctttagct gttaattctt tatctttttt ataatctttg
     2041 tacttattta aagcttttat tatctcaatc ttctctagtt catcaagtgg aattatccta
     2101 tcgcttttaa cttctaactc ttctacagag gtattcttaa ttttttgagg aattacattc
     2161 attgtgatta ttgaagaaga agacatattc acactatatt caataatatt ctgcaactct
     2221 cttatattcc ctggccactt atattccatc atagcatcaa caaccctgct atctatgtca
     2281 ataacatttt tatctaattt ttttgaatac tctttaacca tatgcttgat tataagtggg
     2341 atatcttctt ttctctctct taaacttggt aattttatag gtataacatt taatctataa
     2401 tataaatctt ctctaaaagt gccattttga accatttttt ctaaatcttt atttgtagca
     2461 gcaataattc ttacatctat tgagacattt gatttacttc caattttatt aagttccttt
     2521 tcttgtaata ctctcaataa ctttgactgt aaatgcaaac tcatatctcc tatttcatct
     2581 aaaaatatac ttcccttatg agctacttca aacattccaa gctttccacc ttttttagct
     2641 ccagtaaaag ctccctcttc atatccaaat aattcactct ctaataaatt gtctggtata
     2701 gcagcacagt ttacagctat aaaaggatta tctactctat tgctatggtt gtgtatggct
     2761 cttgcaaata gctccttacc cgttccactt tccccagtaa taagtactgt tgatgttgat
     2821 ttagaagcta ttagggcttc ctttttagaa tttcttatag ctacactttc tccaattatg
     2881 ttttccaatt ttattctata atctttattc atcttattgt agttttttat agcatctctt
     2941 ttatctataa aatcaataac atatcccttt aactcatttt ttaatactat tttattgatg
     3001 ttgtatattc ccttcgaatc atatccgtat cttttataaa aaaaactaca cgatttgtct
     3061 ttcttaaagt tatttataga aggtttcttt ataaaatcta attcgacaaa tatgtttttg
     3121 ccagttatac tatcctttat attgaaaagc tctttaaatt ttgaattata tttatcaatt
     3181 tttccatcca catcaataga cacaacagct ttgtccatat tgtttaatag tgtctctaat
     3241 tttttctttt ctaattcaag ctcataagtt ttaatttgtg cttttaattt attagatatt
     3301 aagtctgcca ttttgcctaa aaagtttata agaccacttt tattattttc tataatttgt
     3361 gcttgttctt ttgtaaaggc tataaggcct ataacgccat gagaatcgcc atcacatata
     3421 ataggacaac acacctctgc aaactctaca caagccccac ttttatagca ttccctacat
     3481 acatcactac tctttgggtc atcaattagt attgtgatct gttctgtaag tgatttttta
     3541 aaagccgtat atttatctac tttctcacct attttgtcta tatatactcc tgtacctgca
     3601 atcctaaata actccttatt tactatagtt acatctatat tgagtactga tactatagct
     3661 tcagctatat tttgaatatc agattttata tattctaaac ctatcataac catcattcct
     3721 tatttttctt taacataagt atttgtctta tttctttaag aatcaagtat atcttattta
     3781 aagtaaattg gattgacatc aggattacaa agagcataac aattattatt aactcataat
     3841 ttatttccat ctaaacatct ccttataaca aaagactagg cttaacctag tcttttatat
     3901 tatatccata tttatatttt aactcattta tcttcttttt cccacataaa tatattttaa
     3961 tttatttgac tctttaatca taccatcaaa gtttaatctt gaaaatatat taaatacttc
     4021 atgtggatag tgaatctttg caacagaacc acaaaattta tgtttaagat aatttaactt
     4081 aacttttaaa ttctttaaat ttaaatcctc taatataacc tctccattgc cctttaacac
     4141 tctttgtatt tcagatgaag ctttttcaca atctgtaata acattaaatt ggtcaactat
     4201 tattatcttg ttgaactgat tatcatcaaa tggtagaaac tctaacttac ctttaactac
     4261 attgcaattt tcattataca ctagagaata acagacatct tcataattac tattctccag
     4321 tatagttaca gaagttccaa gacctctcaa tctcttgcct aggtttccta tatgaccaac
     4381 aagcaatatt ttatcaccta cattatactc aattgtatcc aaaaaacatt ctaattcatc
     4441 agatgtatat ttagctttaa ctggctgttt atttatctga cataatatgc ttttcacaat
     4501 gcttcacctc ttcatttcct caattataaa ttatgtacag attacattat aataaatggc
     4561 gagcaactat tcaagagata tttcattagt atttttttaa acaatcttta tacattttaa
     4621 tatgatatta taccaaattt attaggattt tattcataaa aatcagtcta ttttttatgt
     4681 ttattcattg ttattttcat ccaatttttt tattttcgat atggatactt catatgccat
     4741 ttttttgacc acattatttt catctatttt tttctcatat tctctacttt gaaccctacc
     4801 ccaaagctgt attctatcac caacctttag attctttgca aatttagcat ttctacccca
     4861 aactattgat ggtatataat cagatttatt ataaggtctg tttatagcaa ctaataaatc
     4921 ggttatttcc cttcctaaag gtgtcttcct atacacaggt tccttacata catatccatc
     4981 taaaaatata ctattagggt ctttattttc ctcgtctatg ctttttattt ctctgacaaa
     5041 cactgtaagc actaatctat ttttattatt taatgttttg ttatatgatc ttaattggcc
     5101 tacgacattg actaaatttt gtttatcaag gtcaatatct tggagaagtc tttctgatac
     5161 agtcattgga agtatatcaa aagaatcgct tagtctgttt atttttattt tcatattata
     5221 aaatttttct ccaaatatct catggctaaa ttctagctta ttgtctaact cccctcttaa
     5281 gtttactacg ttattctcat ctttaacagc tatcatattt tatccctcca caattgttaa
     5341 ttcttatatt tctggtatca gcttatataa taaatatatt tataagggat gtattttatt
     5401 acaaatcttt gattgctgtt taaaagtttt tatttagtta taaatttcat ttaatatttc
     5461 acttttataa ttataaaatt attatctttt tgtactacat atactaatta aactacctat
     5521 ttcagttttg tattaattat aaaaatccta ttatataatt aataagtcat atataactta
     5581 aatttattca ctctatataa gcaacttcaa cgaattaaat ttattcaaat aattcagatg
     5641 aatattgagt cattataaac ttatttttct taattataag ttatattaga cttattacag
     5701 cttatatttt tattaatctg tattaatata tgaacattta ttgaacttat tgattataaa
     5761 atttaaatat ttagctttaa attatgcttt attctttgaa aatacaacgt tttttacatt
     5821 aaatctacta aatcactata tatattacat aattggcatg ttaattgctt tatattaatt
     5881 tagcaaataa aaaactgccc taaaatatta attcaatatt ttaaagcagt ttataatctt
     5941 tattattagc tgtaatattt aataaacact aagtagtctt taactaagca gtctttttgt
     6001 ttttatttgc agccattatc attgtagcag aagtataagc tataactact attactaatg
     6061 cttttaatat atccattggt agagttttta ctatatatgc agctacgaaa actcctatta
     6121 aaccacatat agctattata actgatgctt ttttagtata tgcatcttct tttataaatt
     6181 tcatacttgc aactggcatt aataatgcac aagaacccat aactattggg aatgcaactt
     6241 ttgcagacat tcctaagaaa taaaccattg ccatacctgg agcatataga cctataccag
     6301 ctgtcattaa agctcctagt ataaagttac ctattacacc tattaccaat ttaacaccag
     6361 taagacctaa ttcatttcct ccacctggta atacgttaat ccaaggatga cctaataaca
     6421 ttaatattgc tgttaatgct aaagttacac ccataacaag ttgtattttt ctctcgtcca
     6481 tcttagctat tattcctgca cctatatatg aaccaactgc tgctgccaca accatagata
     6541 ctaaagtaac accatcaact ggtatgattg atgtaaatat gaatgcttct gtaacaactg
     6601 gaatagtatg tgctacgttt aatgtaccag gcattttttt atctggtata tttaacttta
     6661 aagcctttga cattgctatt gttggagcaa atgaccctat tcctaatgta tcaaagaagt
     6721 ctgttataaa tcctgttaca gcaacttttg gagtactttg ttcttctagt ttcccctctt
     6781 tgtttgcttt agctacgtct ttaccaaaca caactgcaaa gattgctgcg aatactacat
     6841 ataatgcctt taagattgct accataatcc atcctcctat ttttttattt tttcatatat
     6901 tctgaatctt atagcctaaa agctatagaa tagcaaccga caagtggcta ctattctatt
     6961 ttaatttcct aatttattta ttacttattt aagaataaat ccatgtttaa gtgggtcttc
     7021 ttcatctatt acaaaatgat taaatccagt tatataagca gaaccagtta ttttaggtac
     7081 tacagcattg aaatctgcaa ctttagtttc ttcaactatt tcacctttga ataaagttcc
     7141 taatatactt tcatatacga atttttctcc tacttttaat tcacctttag catgtaaagt
     7201 agctaattta gcacttgttc cagttccaca tggagatctg tcaacttgac cttgaccaaa
     7261 tataactaca tttttgtaag tagcttctgg atgagttggc tcatcatata tttcaactaa
     7321 gtctacagtt tttatatgag ctaaagttgg atgttgtatt tctattttct catttattat
     7381 atctctaagt ttcatagcta gttcagttaa tttaccagca ttttgaggtt cgatttttaa
     7441 acctaattga cttgcatgta ttatagcaaa gaagcttcca ccaaatgata tgtcaaattt
     7501 aacagtccca acacctggta agtcaacttc aacaccttct ttatataaga aagctggtac
     7561 gtttaagaat gatacttctt tagcttttcc atctacaact gtaacgtctc ctcttattat
     7621 tcctgctgga gcttccataa ctacatgagt tacaggctct actgctggaa ctacacctgt
     7681 ttctatagct gctgtcatag ctcctattgt accatggcca cacatgttaa ggtatccacc
     7741 accatccatg aagattattc caaagtcagc atctgggcaa caaggttgag tcattacaga
     7801 accaaacata tcattatgtc ctcttggctc taacattata gcagttctta aataatctag
     7861 attttcctct aaatattctt ttttctcagg catagaattt ccttttatgt taggtattcc
     7921 acctacaact attctagttg cttcacctgc tgtatgagag tctatagctt gtatacttct
     7981 gctaaatttc atatcaaatt cccccttagg tttattttgc gtctttacgc atttttaatt
     8041 ttaggaaagc caatgctttt ttaacatcaa cttcatccat gtgattgtct ccaactactt
     8101 ctgtttctat gtagcctgtt gtctttttat aatcaattat ggtagcttta tccaaatatg
     8161 gattggtaat tactaatcca tctgctccca taatagtaag acctactgca ggtatatctg
     8221 attttacaaa ctctgctata gcatttgtaa aatcaatgtg ctggtttcct gccatatcta
     8281 aagttataat cactccatca ggtcgcaatg attcaatcca tcttccaagt ctagttgcta
     8341 aaaatgtttt ccattcattg tgctctgatg tcccaactac aaccactcct aacaagtcta
     8401 tgtcagaatc attatctatc aaatctagta acgggtctct aaatatatga agtgatgtgt
     8461 ttttcattga tgtagaaatt cccacaaaac tcattccctt acttaaattt tttatccatg
     8521 ttttctcatt ttaagtttta aaaatgctaa agctttttta gcatctattt catttgtgtt
     8581 gttttctcca actacttctg tctcaatacc ttcctttgat ttgttcatat caactatggt
     8641 atccatatat tgattgctta caacaaactt agcttgagtc ccattaaatg taactccaac
     8701 tacaggtata tctctttttc ctatttcctc aaatgtgtta gcgtagtcaa catgtgagtt
     8761 tccccaacca tctgatgaaa ctatagctcc atctactctc atggcttcca gccaagcggc
     8821 cgttctttgt cccacaaagg ttttattttc atttccatct ggagttccta ctactattac
     8881 tccaagtaaa tctacatcct tatcactttc aactatgtct aaaagtgggt ctctaaagtg
     8941 atgaagagat gtttcttttg ttgaaggtcc tattcccata atatccacct ctcttaagtc
     9001 atagctctaa gggcaccatc tctgtattca ttaggagata taatcatagg tacatttccc
     9061 atatctataa ttgatgttcc tccttcaaat ccactaggtt cctttgagaa taactgattg
     9121 tcatacatag caccttgacc tgctatttgt tttactatga caaccttttt tgcatttggt
     9181 cgaattttgt caaaatattc acgaacttga gtagcatttc ttccgtcttc cattttcaaa
     9241 gacgctctta tttcttgtat aaaatcatca catgctctga atgctgcaag tggaagtgtt
     9301 ctttcaaaag gtaaacctcc ctttaaagtt acatctacat ggattatata atcttcaact
     9361 gatggagttc catatctccc aaataccatt tggtctttca aaattccttc tgaagaacca
     9421 aattcatgca tttgatttcc atcttcatct gcaccagtta acattacata tacacccgtt
     9481 aaagtatgtg tgataccctc tcccagtctg cctaaaactt ttgttgatat tggtataatg
     9541 tccataatag tattaatttc tctatcataa tctccaggtt ttataatgtc tacttttatg
     9601 tctgttatta actcttctct ttcttttatt tcatctatac ttgataaatc tattgtaagt
     9661 acatctttct ttatagaagt ttttgaaccg aaattaactt cattcatatg aaacggtttt
     9721 ataacaagtc ttctcattat tttttcttcc ataatatcca cctttgttct taaaacccct
     9781 ctcctaaccg tgtaggagaa gggttctata ttttcaaata taaattacat tttaaacgtg
     9841 agctttatat tcgtatggta atggaactat cttaccagca gatggtattg caactaattg
     9901 ctctaatgta tctcttaata tatgtgtttg catttctata ttatgtggtt caccagcatt
     9961 agcacccatt ggaactaatg gagcaactgc tctaggagtt ccgttttgtc taactactgg
    10021 aggaagagct gctattatta ttgtaggtat tccagcttcc tctattgctc tctgcacgat
    10081 cacggcagtt ctatggcaag tacctcagcc agctgtcata actacaccgt ctactccctc
    10141 ttccttaagt ttagcagcga tagcaggacc agtttcttca gtgaagacat gttggtttcc
    10201 tccaccaccc atgaatgcat agtgcatagg agctactcct ttgataaatc cttctgcagc
    10261 taattcatgt aatctatcta tagggaacat acagtttata tctttgttta catctccatt
    10321 gtcatatcca ccatgtgata ccatcatttc atcaactgta gctgtattag gtatagctct
    10381 aaatgtagta tctcctgcta gattaaatct cttgtcagat tttaaatgaa caccagctgc
    10441 tgtagctaaa gctatagtca tatcttttaa ttctttagtt acaggagtcc aaactggagg
    10501 aggtgttatt ggaacgaata tttcagattg aagtccttgt actgttgtaa ggctcatata
    10561 tttcctctcc cttctatttt caaacgatta tttattattt ttcttcttgt tttgattgca
    10621 tttctgcttg tttttctttt tctctcctaa tattttctac atactcttga ttaggctctg
    10681 gacttaatac ctcaggatag cttagcatga atccaacttc ttgacctatc ttaagttcgt
    10741 agtcagttat aagcattcta ttaggtattt taaattgtcc aagtctacct tttaggtcta
    10801 tagtcactcc accacctttg atgtcaacta taataccttc ataaaaagtc tctgatgaga
    10861 tatattttaa cctatccatt ataattctac ctctctatac tgttttaaac ttggatctaa
    10921 atttatgtta ttgtctaaat cttagttagt atctaaattt tagttttcgt ctttttcgta
    10981 tatttctctt ctcttcttac tcattggtag agattgctca ttctcttcta atactatttt
    11041 ttctcctgta actttttcta tagcttctat gttatttaat ttaacatttg gattccattt
    11101 tctttcagga gctttaactt ctacaccttc tatagcattt ttaagcatag ccattattct
    11161 aacagcatct tctggagcta aagtgttgtt agataatatt tcattctcta taccttgctt
    11221 agacttattg ttgtctacca tgtgagtcat gtatttatta ccaacaacta gagcaccttg
    11281 aacagctgag aaacttacac caactactgg tatacctctc atacctattt gctctatgtg
    11341 agatgcgaaa tctatatggt tgtttccgaa accttcagta gttactacag ctccatcaac
    11401 ttccatagct tcaactaaca tacctaatct cttagataca tagaatttct cagcatttac
    11461 ttgtggagaa ccaacaaata caacacctac taaatctatt tcttcgtctt ccatagctct
    11521 tattactaat ggctctctcc agtaatgtct tgacatttct tttgatgcag gtcctataca
    11581 agttaatgca tggataccac catctaatac ttcaagtgga gataatacaa ctggcatgtt
    11641 tcctaagtca acgtttggtt gagctcctaa tgtaccaact ggctcaactg gtaatattag
    11701 gttatcatgc attgcacctt gtcccattat ttcttttata acaacaactt tctttttacc
    11761 ttctcttctg tattgctcta ttacttcttc atcaactact aaagagttgt caactttctt
    11821 taatgcttct cttatttctt gagttacata gtcaaatgca cggtgagcag ctaaaggtcc
    11881 tggtctttcc atgttagttc ctgcttttat tgttacttga cctttgatga atatttcacc
    11941 tttgtcagca gcacctggtc taccccacat tatagtagta tttaactcac cttctgaaga
    12001 accaaattca cctatttgaa ctccatcttc atctgtacca gttaatacca ttacagctcc
    12061 atctataact ctagttatac ctgaacctaa ttcgccttct tctttagtag cgattggttg
    12121 tatatctaat acagcttcac tgtaagtttc atatttatca ggagttatta tttctaactt
    12181 catatctaca actaactctt gagtttcatt agcttcttta catatttctt ctgcgtttct
    12241 gatgtataaa gtagttcctt ctattttagt ttcatcagca aattcaactt tatctatttt
    12301 aaagtgtttt ttagttaagc ttcttaaaag tttcttttct tcaacttttg gagctacttc
    12361 tgctgctgca cttgccactg gagcagcaac gtttgctact ggagcagcaa ctcctgcttg
    12421 accagctact gataaaggta tctctaagct tatgttctta ccttcaccta tgtgtatttt
    12481 aactgtttca cctgcaactc ctgtgcttac tggaactact gctgcttctg atactggagc
    12541 tgcttcttct actgtttctt ctactacttc ttctttagcc tctcctgctt tgtatccttc
    12601 tacgttatct gtagtcattg gagaaagtgc atctaaagtt tcttttaact tagctcctag
    12661 tacttgacct atagttaatc cattttctgg tattgttaat aatccagaat cctctaagtc
    12721 tgcaaatatt gctggatctt ctaagttttc tggcgctata attgttcccg cttcaaatct
    12781 acaacaacaa actgctgggt catttgcatg ggcttgagct gtttctaaag ttattgacat
    12841 agtataaaat acctccttac attattttct tatttattat tttaaatctc aattcctcaa
    12901 caggtaaccc cttaactgag aaattaagta aattaaaacc tacataattt gttatgatta
    12961 atattatttt tttctcactc aaagtcactt tatattataa gcaattccta tgccaactta
    13021 ttaacaaaaa gattttttct ataatagatt cttcatctat cgctattttt agccaaatat
    13081 tatataaata tttatttaaa atatgttatt ttttgtattt gtaaacattt tctcaaacaa
    13141 aaaaagtgtc aattaatcaa cactttttca ttttttttaa cacatctata tacacaacac
    13201 ttatacaagt gttggcttta ctaggtttta aattaaagtt tatcattttg tcaaaattta
    13261 gtcatatgtc atattttaga cacatctatt ttgtattggt ctatcttata atacaaagtt
    13321 gttctgggta tatttaatat cttagctgct ttagctttat ttccattaga cattttaagt
    13381 gccttggtta tattctttat ttcaattttt tgagttgcct tcgttaaatc taagggatac
    13441 tcttcttctt cagtactatt atttgtagaa tccatcatat aacttggtac atcatctaca
    13501 tctattttag aattttgtga caatacaaca atattttcaa tcgtattttt taattctcta
    13561 atattacctt tccatctata attctgaaat atatctatta catctttgct aattgtaaga
    13621 acaggcttat tattttgttt acatatctcc tctaaaaagc tatgaaccaa taatcctatg
    13681 tcttcttttc tctctctaag tggaggaatc tttatttcaa caacatttaa tctatagtat
    13741 aaatcttctc taaatttttc tgcctttacc attttactca aatctttatt ggtagctgat
    13801 attattctag gatttatttt tattgtggta tctccaccaa ctcttcttat ttctttttcc
    13861 tgtaatactc taagcaactt tgcttgcata ctaagaggta aatcggcaat ttcatctaaa
    13921 aatacagttc catcttttgc aagttcaaat atacctattc gacccttttt acttgctcct
    13981 gtaaatgcac ctgattcata cccaaaaaac tcactttcaa ataactcatt tggtatggca
    14041 ctacagttaa caggtataaa ttgacctttt ctctcactat aatcatgaat agctcttgca
    14101 aatacttctt tacccgtacc actttcaccc caaataaata tgctactatt tgttctagcc
    14161 acttgttttg caatcgcttt cgatttttcc aatttataac ttttacctaa aatcttatca
    14221 aaactacctt tagataaatt tttgacttcg ttctttagat attttaatgt atcatttact
    14281 ttttcaagtt caagagctaa tatcttagct tctgtaacat caacttctgt acatacaaca
    14341 ccgataaatt cttctttata aaatatagga tttgcatgaa ctaatgcata catattttct
    14401 tttttatctg taaaatacaa gtcactcatt ccaactttac tatttagtac tttttctgaa
    14461 atagtatttt ccaaaaattc attcatcggt cgaccaacta tatcttcaga ctttatatca
    14521 tacctttcct ccataaactt attccacaag ataacaacac cttcgttatt tatagcacaa
    14581 attccttctt taatttgacc tataatatac ttcaaagtca atccataatc ttctaaatgc
    14641 atatatagat agtccctaat gtgctcttgt tttagaactc caataatttt attatctcta
    14701 agaacaggaa gaatccctat attttttaat atcattatat ctctacattc atctaaggtt
    14761 aaaccttcgt tgacatatat tacatccttc ttcattataa gttttactgg ttgaccttca
    14821 tactttttat gcatctcata aagattatgt atatccgtca tggatataat gccttttaat
    14881 tggtcagatg aatctattac cattagagtt tttgtgttgc tttttatcat ttcttttatt
    14941 gcatcttcaa ttcttgtgtc ttcgtctata gttgtaaatt ttgtatccat tacctcttct
    15001 acttttttta cttctggtat cgaaaacata aagtcccccc ctttatagta gtacctcatc
    15061 aaaacttatt tatatatatt tattctagca tattataata attatttatt aaataattat
    15121 tattaaaatt tattaagtaa ttagcattta aatttatata atcactttaa aatttataat
    15181 taaatttata caaatgaaat caaatgaatt atacccttag tatattattt tttcaatact
    15241 attttcacct tcaaaacatt tttctattca tcaaacaatt aatgaatgcc tgtatccaat
    15301 cataaattta agaatataat taaaatagat atttttaaac aaactatatt tgcatattat
    15361 atttacataa aaaagtccca aaatgactta taaatcaatt tgagactttt tattttaaac
    15421 ttaataataa tataaattaa tttcttttta taaattaagg cgtaaaacca ctataccttt
    15481 aggttggtgg atgtaaacct atcccttact ttgtatgtat ttttcaatca tttctttaga
    15541 aacatttcct acactgaaag caaagtatcc atctgagcaa aaagtttttt ctgtatttac
    15601 atacaaatat caaatgtacc aataacaaac acttagaatg gtttctacta acataattca
    15661 caactccaat tcaatccctt cttaaatata tatggtttaa ttagttttac atttggcact
    15721 tttttgattt cttttaaagc tatatattgc cctcaaaata cattaaaaaa catccttaga
    15781 agtaattttt aagttttact tctaaagatg tctaacttct atttatctat tattttattt
    15841 ttaatcttac gtcacctttt attcttctta ttaatgggtg attaactgtt ctcattacat
    15901 ggtcagtaat atgatataca cctaacttta attttggtgc agaacccatt actgtgttac
    15961 tacagtcagt acagtttcct attaatataa cttctgctcc agctttctta aattcaatac
    16021 atttttgagc ttgtcctgga cagtgtcctg gattttcaca ttttaatgca cctttaattt
    16081 cttgagcttg tttacatttt tgaactgata ctatatcagt aactcccatt ttagttgcta
    16141 tatctctcat tctctcttca ttaggtccac atgcacagac taaaagaccc atttttctct
    16201 tttcattaac aaatggcata gtaactatta taccaccact agttttagtt attggagcat
    16261 ctaaagtagt agctttacca gtgaaaggtc ctcctaggat tatttcacca tattcacctt
    16321 ttattccacc agctctttca attaattctc cgacagttgt tccaattggc acatcttcaa
    16381 atactataga ctcttttcca ctgtttaatt gaccaactac agttatattt ttagatataa
    16441 ctggtctttt ttgctcaaca gcctctacta ttctagttag tgtttctacg tttattacta
    16501 ctgcattagc ctctgaaggt aattgagttg gctcaagtaa ttttcctaga acgtctctta
    16561 ctattgctct ctcttctccc attgggtaca tatcaactag ttctgcaact tctatgttgt
    16621 ctcctggctt tattactttt ttgaaagctt ctattgcttt tggatgcttg ctttttattg
    16681 ctagcatacc ttttccagca ttagtaactt ccatagcata ttttatacct tgataaacta
    16741 tttctggtct ttcttctatt tcttttatat tatgagctaa taaaggttca cattcagctg
    16801 catttgctaa tattgttcca ccatttaaat ctatgtttaa ttttacatga gtagggaatc
    16861 ctgctccacc cattcctact actccagctt ctttaattaa gtcaagtata ccttctcctt
    16921 ttaatggttc aaaagtatct tcttgtactt catcagcttc aattactatc gcttggtcat
    16981 ttatttcact tatttttcca ctaacactgg cataaatatt tgcccctaat ccagttggtt
    17041 ctgcgattag agttcctttt tttactacat ctccaacact tactactggc ttatctggac
    17101 caccaacgtg ttgttttaat aaaatagaat atagtttgtt caactagtac gcccccttaa
    17161 gtatattaat ttttttataa gctgttcaaa taatgttaat gctatttttc tattataagg
    17221 tcttaacata ttgtttattt ctatagaaca tatcgcttat taacaaagca atttctatgc
    17281 caacttttaa acaaacttat accctttgga aatactgtct tacagcattc attaattctt
    17341 ctaaattttt aaatccatca aaaaaaagtc aattattaga cactttttta atcaatttaa
    17401 tctataatta ttgatattgc atacatgtat aaaaagtgtc aaaaaataga caccgtcttt
    17461 ttatcgacac ttttctaaca tatactatat aatttatcac aactttactt aaaatataca
    17521 tttattactt attaattctt agttttttgc actccattgg aatcaactac ataattatct
    17581 ttatacaaca aatcagatat tttgactaga ctgtatccat ctttttttag tcgacttatt
    17641 atagtatcca aatagttatc tacatcattt atattagcat ggaataaaac tattgaacca
    17701 ggttgagatt cttttattac tttctcaatt acatgatttg gtccaagctc cttccaatct
    17761 atagaatcta catcccactt aattacttta taacctaaat cactacaaat gtccatagct
    17821 ttattgttta cgtccccaaa aggaggtcta aataaattag tcttttttcc tgttatatta
    17881 tatatttttt ctgatgtagt ttctatctct tttgttatgt cttcttttga tatttccttc
    17941 aaattggcat gagtgtttga atgatttcca atttcatgtc ctttttcatc tatagctttt
    18001 acaatttctt cattatcatc cacccaacta ccaactaaaa agaaggtagc ttttacatta
    18061 tgtttatcta aaatttttaa aatatcatcc atattatcag ttccccatgc tacatcaaaa
    18121 ctcaaggcta tttttttctc atttgtatca actttatata taggcatttt tgaattgtcg
    18181 tatttagaac atatgtattt tattccataa acaaaaacaa atattaacac taataataat
    18241 gaaaaaaata aaacactata tatcaaagct ttagtccttt taacttctct tttcatacta
    18301 ttcccccttt gaaataattt cttctatatt taaaatttat tttttttcag ctcaaaaaag
    18361 tactgtaata tttaatttat aagacaataa ctaaaaaaat aaaacctata ttgacctaat
    18421 taaactctct aaaatactca tttatttaat ttttttatat tttttacatt tttaccttta
    18481 tttttctgta tatattattt ttattttgga gaaatt
//
LOCUS       DAEPOZ010000054        18287 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.054, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000054 DAEPOZ010000000
VERSION     DAEPOZ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18287
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.054"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(217..711)
                     /locus_tag="KR440_001461"
     CDS             complement(217..711)
                     /locus_tag="KR440_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBH0698517.1"
                     /translation="MSTLVTLLGLLVCTKISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKLSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPRMPLSVSLALAAVISSTDLVAVKSITQGLNFPKNMMSILEGESLLNDDDRII
                     NIME"
     gene            complement(863..1834)
                     /locus_tag="KR440_001462"
     CDS             complement(863..1834)
                     /locus_tag="KR440_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBH0698518.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKEIKCVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPDEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTRALMSAVPVQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            complement(1835..2812)
                     /locus_tag="KR440_001463"
     CDS             complement(1835..2812)
                     /locus_tag="KR440_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0698519.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMVIRGNEISMIFQEPMTS
                     LNPAFTVGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPVLGKNKDKRLYSIPGQV
                     PNPIGIPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            3031..4590
                     /locus_tag="KR440_001464"
     CDS             3031..4590
                     /locus_tag="KR440_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0698520.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKRKEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            4696..5655
                     /locus_tag="KR440_001465"
     CDS             4696..5655
                     /locus_tag="KR440_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0698521.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPVYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLVKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            5658..6470
                     /locus_tag="KR440_001466"
     CDS             5658..6470
                     /locus_tag="KR440_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0698522.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIIASFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVISLTIISKKFSSNT"
     gene            complement(6685..7587)
                     /locus_tag="KR440_001467"
     CDS             complement(6685..7587)
                     /locus_tag="KR440_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0698523.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            7942..9120
                     /locus_tag="KR440_001468"
     CDS             7942..9120
                     /locus_tag="KR440_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBH0698524.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVGLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINSGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLAKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            9129..9785
                     /locus_tag="KR440_001469"
     CDS             9129..9785
                     /locus_tag="KR440_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HBH0698525.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            9785..10450
                     /locus_tag="KR440_001470"
     CDS             9785..10450
                     /locus_tag="KR440_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HBH0698526.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     ILGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            10555..11331
                     /locus_tag="KR440_001471"
     CDS             10555..11331
                     /locus_tag="KR440_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HBH0698527.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            11393..12727
                     /locus_tag="KR440_001472"
     CDS             11393..12727
                     /locus_tag="KR440_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBH0698528.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVLLNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKVGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSKLEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            13224..14150
                     /locus_tag="KR440_001473"
     CDS             13224..14150
                     /locus_tag="KR440_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HBH0698529.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PDNNKPESNKPEDNNNSGSTNKPDNNKPESNKPEDNNNSGSTDKPNNNKPESNKPEDN
                     NNSDSTNKPDDNNNSGSTSENFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVATKK
                     SQDMIDNNYFSHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSEGH
                     RKNIMNPNFSKIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(14542..18081)
                     /gene="nifJ"
                     /locus_tag="KR440_001474"
     CDS             complement(14542..18081)
                     /gene="nifJ"
                     /locus_tag="KR440_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBH0698530.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHACIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRIQV
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKVDVMPANT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMDICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVLEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
BASE COUNT         6380 a         2854 c         2664 g         6389 t
ORIGIN      
        1 cttattcata aaaaaatacc taaattaaga tacaattcac ctgtatacct taacctaggt
       61 attgcctgct taccagtaac aatccagctt gttaattctt atatattgta ttcaatttat
      121 ggttttattc tattataaat aaatatatat gtcaagtaaa ttattgaaaa ctatttccta
      181 ttttttagta ttatttttaa catcaaaaat caaattttat tccattatat ttatgattct
      241 atcatcatca tttaataaag attcaccttc aagtatactc atcatatttt taggaaaatt
      301 taatccttgt gtgattgatt ttactgctac taaatcagtt gaagatatta ctgctgccaa
      361 agcaagtgaa acagataatg gcatcctagg aataagaaaa tgtataaaga ttccacctgc
      421 aaacacggtt atcaaaacta aaccaaatgc aagaagcaaa attggttttc taagctctag
      481 taactctttt cttgatacat tttgtccttc actgaataat aatggagcta tgatacatat
      541 tacaaatatt tctggattaa atgaaaatga taatttaaac ggcaaaatag acaatataat
      601 ccctagtaca atttgatata ttgcaagagg aatattactc cactttttgc taaatacagt
      661 tgaaatcttt gtacaaacca ataatcccaa caatgttact aaagttgaca taactacctc
      721 tcttttcaat aataatatta taaattaaac ttatatatca actaacttaa tattaacaac
      781 atgaaatccc atcttaatca aaaaaatttt cagtttattc tattattctt attatataca
      841 tcaaatttaa tttttgaaat atctatacta gatggcatgc aacaaaatga tttcttgata
      901 tttcttttaa aactggaatc tcttttttgc atatttcctt acaataaggg catcttgtat
      961 gaaacttaca tccacttggt ggatttgttg gagatggtat gtctcctttt aatataattc
     1021 tatttttctt aattgtaggg tcttgtactg gtactgcgct cataagtgct cttgtatatg
     1081 gatgttgaga atgtaagtaa agttctcctt tagttgcaat ttctattata gaacctagat
     1141 acataacaca aactctatgt gcaatatgat ttactacact caaatcatga gatataaata
     1201 aatatgagag tttatattta tcctgtaaat ccataagtaa atttataatt tgagattgaa
     1261 tagaaacatc tagtgcagaa acaggttcat cacaaactat aaattctgga tttaatgcta
     1321 atgctcttgc tatacctatc ctctgtcttt gacctccaga aaactcatct ggttttctat
     1381 ttaaatggta cttagaaaga ccacaacttt ctacaacatt taatatatta tcttttattt
     1441 cattctttgg cactaaatta tgttctataa ctgcctctga tattatttgt tccacattta
     1501 atcttggatt taatgaacta tatgggtctt gaaatatcat ttgcattttg ggtcttaact
     1561 ttctaagttc ttccttctct aacttatata tatctacacc attaaattca actttacctt
     1621 cagttttttc aattaatcta aggatggttc ttcctgcagt tgattttcca catcccgatt
     1681 ctccaactat agccaaagtt tctccttttt ttaaatcaaa agaaattcca tctactgcct
     1741 ttacacattt aatttcctta tcaaataatc cagatttaac ttcaaagtac tttttaagac
     1801 cttctacttt taataaactc tcttcttttt tcaattatat ctcctccttt ttatataacc
     1861 aacatgcaat actatgacca ctattcaatt ctattaaagg aggtatctga gttctacatt
     1921 tatcacatac tttttcacat ctatcactaa aataacatga atctggtatt ccaattggat
     1981 ttggtacttg tcctggaatc gaatacaatc ttttatcttt gttttttcca agaacaggct
     2041 ttgattttag aagtccaata gtataaggat gttttggatt tttaaataat tcaacaacct
     2101 ctgccacttc tataatttta ccagcataca tcactaatac tttgtcacac atttctgcaa
     2161 ctactcccaa atcatgtgtg atcatcatta tagatgtgtt taatttttct tttatatttt
     2221 tcattatatc taaaatctga gcttgaatag taacatctaa agctgttgtt ggttcatcag
     2281 ctataagtaa ttttggatta catgataaag ccatagctat cattattcgt tgtctcatac
     2341 cacctgataa ttcatgtgga tatgaattat atatttcttc agctcttggt atttctacca
     2401 gttttatcat atcgattacc tttttcttag cttcttcctt tgataaatct tgatgtatca
     2461 taataccttc aatgatttga cttccaacag tgaacgctgg gtttaaagat gtcattggtt
     2521 cttgaaaaat cattgatatc tcattccctc ttattaccat aagttcatct tcattcatct
     2581 ttaaaatgtc tttgcctcga aagattattt ctccaccttc tatttttgat gttggaagta
     2641 acttcatcaa agacatggct gttacagact taccacatcc tgattctcca actattccaa
     2701 tagtttcacc ttcatttata ttaaaactta cttcatttac actctttaca atccctttat
     2761 ctgtatgaaa ataaactttt aaatctttaa cctcaattag tgctttactc attattttct
     2821 cctctcagca aaattactta aaataacata caccttttga ttgtatttta taaaaattta
     2881 aattacaaca ttttcttaca ttttattatt tttttctatt aattataata atataatagt
     2941 taatttctat atatattgat ttaataatga ttactattat acaatgaaaa atatgtgtta
     3001 aatattattt tatggaggtt ttataagaat atgaaattta aaaaattggc atcattaatc
     3061 ttagtttcat cattaatgct tacatttaca gcctgtgctt ccaatgataa agacaagtct
     3121 agtggaagca aatctggtgg aactgttgta atacctatgg caagtgaccc agatataatc
     3181 aatggagctt ttgcaaacgt taaagaagac tctcttgcat caaatatagt ttatgctcct
     3241 ctttatactt atgaaaaagg gaatttagta aattatttag cagaaaaagt agattttaaa
     3301 gatagtaagg aattaactat aaaattaaaa tccaatttaa aatggcatga tggaaaacca
     3361 ataactgctg aagatgtctt atttacattt aatacagttt tagacgaaaa acaaaattca
     3421 ccatcaagac aatacttatt ggtgggtgaa aaaccagtta aagtagaaaa aattgatgat
     3481 ttaacagtta agattacttt accaactgca agtgaatctt tcttatatgg tatatctaaa
     3541 atttctccaa taccaaaaca tgtatttgaa ggagaatcaa atatagctaa gtcagaaaaa
     3601 aataataatc cagttggttc tggtgcattc aagtttaagg aatggaaaaa aggtgaaagc
     3661 atagtatttg aaaagaatgc agattatttt ggtggagaac ctaaggcaga ttctatagcg
     3721 ctaaaaataa ttcctaatga agcatctcaa gaagcagctt taaataatgg cgaaatttca
     3781 cttatgaaga catctgctga aggatatgaa aaggcaaaat caaactcaaa cttacaaact
     3841 tatacttata gtgaagaaag attaaactac attgtattta accaaaatat atcaaacatg
     3901 gctaacaaag aagtaagaca agccttatca tatgccttaa atagaaatga aatgatagaa
     3961 tcagcatatg gaaaagaagg ttctgtacct gctaaatcta tattagttcc agaagcagac
     4021 ttctatacag aagaaggtgt tgaaggatat gaccaagata caaataaggc taaagactta
     4081 ttggataaaa gtggtgttaa aattgataaa ttaaaaatag gttataacac aggtagattt
     4141 gggcataaaa actatgcctt agtagctcaa caagagttga aaaaaattgg tatagaagct
     4201 gaaatagttc cttatgaaag taaggcattc tttaatatac tatttagtaa tagcactgaa
     4261 tgtgatatgt atgtaaatgg ttatgcttgg ggattagaac caaacccata tagaggtatg
     4321 tttgaaactg gtcaatattg caaccaaact aaatattcta atgctgaaat agatgcttta
     4381 tgggaaaaag gatttactga gttaaacaag gaaaaacgta aagaaatata caaacaaata
     4441 caacaggata tatctaagga tgcacctatt tacactatag attatgaaca aaatttaatg
     4501 gcagctcaaa aaaatctaaa aggaataaaa gatgctaaac catctcctgc aatattattt
     4561 gaagattggt ctaaactgta tgtagaataa ttgatgtaaa attttatatt attaattaga
     4621 aaaggagatg tctaaaaata atattgtttt attattttaa aacgagacat ctcttttaat
     4681 taggagaatt tacttatgtt aaaattaata ttgaatagag taaaagtttt aatacctatg
     4741 ttaatcttaa tatctatatt atcatttgga cttttagaat tagctcctgg tgacccagca
     4801 gatgcatata taaatccttt aatgtcttca caggatattg aaaatataag ggttaatatg
     4861 gggcttgata aaccagtata tataagatat ttaaactggt tgaaaaacac tttaagtgga
     4921 aacttaggta tttcttatat aaaccatatg cccgttacag aacaaattat ggaaaaaatg
     4981 ggaaatacat ttattcttat gggtacttct ttgatatttt caatattggt agctatacct
     5041 cttggaatat ttttagctgt aaataaaaat tcaataacta gtaaagtatc atctgttttt
     5101 aattatatag gtgtttctat cccttctttt tggataggta tgatactcat ttcaattttt
     5161 tcagttaaac ttaacatttt cccatcagga ggtatgcata ctattggaaa tgatagtatt
     5221 ggagatttag taaaacattt agttttgcct gtaataactt taggtctata taatactgct
     5281 atttttacaa attatgtaga agctagtgta aatgaacaat taaaaaaaca atatgttatt
     5341 acagcaagag caaaaggatt atcagaaaaa gtcatactat ttaaacatgt cttgaaaaat
     5401 tcactgacat cattggttac aatcctaggc atgagtatac aaaaattagt cactggtgca
     5461 tttgttacag aagtagtttt ctcttggcca ggtatgggaa gattaatgat tgattcaatt
     5521 ttttcaagag attatactgt aattatggca ataactatgt tatcagcact atttttaatt
     5581 ttaggtaatt tagttgctga tatactatac ttattaattg accctaaaat aaaatctagc
     5641 aaaggaggat tctagttgtg tttacaagta tcttaaaaaa attaaaaaaa gataaatcct
     5701 ttgcttttgc atgtattact cttagcataa taatcatatt aattatagct tcttttatat
     5761 taaatataga ctctaatgca attgatacta cttctaaact tcaagaacct agcttaaagc
     5821 atatctttgg tacagacgaa cttggtagag attacttctc tagagctctt tatggtggaa
     5881 ggatttcact tagtgttggt attttatcaa tgcttatctc tgtagtattt ggtactgctg
     5941 taggtgttat tagtggatat attggaggta agctagacca attcttgatg agattagtag
     6001 atatgcttat gagtatacca agtttcttag ttttgattgt attaaatact tatctaacac
     6061 ctaaagtttc cactattata ctaattattg gattcttaag ttggatggaa gtttcaagaa
     6121 ttgtacgtgg cgaaacatta aaaataaagg aaaatgaata ctgtttagct gctaaagcta
     6181 ctggtatcaa gactagaaat atactattaa gacatatagt tttaaactta aaagagacta
     6241 ttgtagtcgc tggttcgtta aatattgcta atgctatctt aactgaatcc gctcttagtt
     6301 ttttaggtct tggaatccaa ttacctcttg catcatgggg taatatgctt caagatgctc
     6361 aaagacatat ttttgacaag ttatatttag ctgtatttcc aggattactt atatttttaa
     6421 cagtcataag tttgactata ataagcaaaa aattttctag taatacttaa caaattttat
     6481 atagaaaata aaaacagtga ttatctaaag atatacactg tttttatttt tcgtttaaaa
     6541 aatacatatt ttgtatatta taactttaaa tactactcta ccatgttatg taaagcctat
     6601 tattgctctc aaacatatag atgaaaaact aatagtttat tgtaataaaa attgatttat
     6661 ttatttgtta ttttaataac atttttaaga gttcttttta ctaaagaaat ctataataaa
     6721 tttaaataca tgttggtcta catggctata acgttctttt ttaggtctac atagtataac
     6781 tttccatgta ataggatgat taaaaggctt ttctataatt ttgctatttt caaaaaaatc
     6841 atgaactggt gatggtaaaa ttgttataaa atcagaattt gtggttgata gcaacaaaaa
     6901 atcccatgaa ccagacatta tatatctttt taacttaaca ttttcatcag taaatttttg
     6961 cattagctta tgatgtatca taaaagtagg attaaatatt gcaagtaatt gctcattcaa
     7021 atcgttccaa tctatcttat ccttcttagc aagtggatga tttatattta aaaatgctgt
     7081 aagttcatct tcttgaagca catgttcagt tactacactt ttatcaattt tatgtggatg
     7141 taacagtact gcataatcta gttcctgcaa gataagcatt ctatttaagt catatgcacc
     7201 cttctctact atatcaaatt ctatatctgg attatttgca actaactgtg caactacatc
     7261 tgaaaatgct attccaagta taagtggagg tattcctatt ctaacttttc ctttaaattg
     7321 aactgaatct tctcttaaat cttccatcat atttttgtat tcatttataa tatgttctgc
     7381 atttacataa aatctttctc ctgctggtgt taacccattt aacctacctt tatacctttc
     7441 aaataaatat acatcttcac tttcttcaaa attttttata atttgactta atgctggttg
     7501 agatatgtga agtgtcttag atgctattga taaattaaaa ccagatttca ctatttcaac
     7561 aaaatatttc aactgtttta tatccatttt aaaccccctt ccccaaaagt ataatttaga
     7621 ttatattttt tcttctaatg atattttata ggaaaatcaa cataatttca ttaatatttt
     7681 aacaaaatat ttttctaaat ctactataaa tttttatcct ataaattttt cttatacact
     7741 atataaaaaa ttagacttag acaaaatgct tttttcgccg taatctataa ttagaaacag
     7801 gaaaacctaa agcattagga ataaaagtaa ttgaaatact taaaaacaag cttgtattaa
     7861 aaaagtactt tattatagtg ttccctaatt tctttattca gaaaattttt aattatcatt
     7921 caagaaaggt aactattaga tatgaaagat gtagtaatcg tatcagcagt cagaacacct
     7981 ataggaagtt ttggaggtgt atttaaaaat acttcagctg ttcaacttgg aactatcgct
     8041 gttaaagaag cgatatcacg tgtaggactt aatttatctg aaatagatga agtaataatt
     8101 ggaaatgttc ttcaaacagg gcttggacag aatgttgcta ggcaaattgc tataaattca
     8161 ggtataccaa attctgttcc aagctacaca gtaaataaat tatgtggttc agggcttaaa
     8221 agtgttcagc tagctgccca atcaatcaca tctggtgaaa atgatgtggt aattgcaggt
     8281 ggtactgaaa atatgagcca agctccttat atcgttccaa ctgctcgctt tggtagtaaa
     8341 atgggtgaca taaccatggt agatagtatg ctaacagatg gccttattga tgctttcaat
     8401 cagtatcata tgggaataac tgcagaaaat attgcaacta aatttgaatt tactagagaa
     8461 atgcaagata aattggcatt agaaagtcaa aataaggctg agaatgctat aaaaaacaac
     8521 cgctttaaag aagaaatcgt tccagtagat gttttaatac gtcgaggaaa aatagaaact
     8581 attgataaag atgaatatcc taaattaggc atgacatttg aaggtttagc taaacttaaa
     8641 cctgcattca aaaaagatgg tactgtaaca gctggaaatg cctcaggtat taatgatggt
     8701 gcggctatgt taatactaat gagccaacaa aaagcagacg aactaggtat tagaccactt
     8761 gctaaaataa aatcctatgc ttcagctggt gttgaacctg aagtcatggg tactggtcca
     8821 atccctgcaa ctagaaaagc actgaaaaaa gctggactta gcatcaatga tatagactta
     8881 atagaagcaa acgaagcgtt tgctgcccaa gcattagctg ttaaaaatga acttcaaata
     8941 gattcatcaa agttaaatgt taatggtggt gcaatagcat taggacatcc aataggagca
     9001 agtggagcta gaatacttgt taccttaatt tatgaaatgc aaaaacgtaa agttgaaact
     9061 ggacttgcaa cactttgtat tggtggtggt caaggaatat caatggtggt ttcacgatag
     9121 gaggaaatat gaataaaata gttagcattg atgaagctct atctcatgta aaagatggag
     9181 atacaataat ggttggtgga ttcatggcca atggttcacc agaaaattta attgattatt
     9241 tatgtagtaa aaatataaaa gatttgacac taatttgcaa tgatactggc tttattgaca
     9301 aaggagttgg caaaatggtt gtaaataagc aatttaaaaa gataattgct tctcatgtag
     9361 gattaaataa agaaactgga agacaaatga acaacaatga aacagatgtt gagttagttc
     9421 ctcagggaac tcttgctgaa caaatacgtg cagctggtta tggtctagga ggtatactca
     9481 caccgactgg tattggtact cttgttgagg agggaaagca aaaaattgtt gtcgatagca
     9541 aagaatacct tcttgaaaaa ccaatttatg cagatgttgc tcttctattt gcatcaaagg
     9601 tcgataaagc aggtaatcta gtttataaag gctctatgaa caattttaat aatttaatgg
     9661 catctgctgc caaaattaca atagttgaag cagatgaaat cgttgaaatt ggtgacattg
     9721 acccaaatga agttaataca ccaggtattt ttgtgaatta catcgttgaa ggaggtaatt
     9781 tataatggat aaattagaaa tgcaagaata tattgcaaat agagtatcta aagaattaaa
     9841 agacggagcc gttgtaaacc ttggaatagg tctaccaacc aaagtagcaa attatattcc
     9901 ggataatgta aacgttatct tacaatctga aaatggcttt ttaggtttag gttcagccga
     9961 agatggaaat tcaagtgatg aaactattgt aaatgcaggt ggtcaaccag ttactatact
    10021 tcctggagga tgtttctttg atagtgctac ttcttttgga ataatcagag gaggtcatgt
    10081 tgatataact attcttggcg cacttcaggt tgacaaatat ggcaatatag caaactatat
    10141 gattccaggt aagatggttc caggtatggg tggagctatg gacctagtaa ctggagctaa
    10201 aaaagttatt gtagcaatgg aacatacttc aaaaggttct gcaaaaatat taaataattg
    10261 tactcttcca cttactgcaa caaatgctgt agatttgata gttactgaaa tgggagttat
    10321 ggaagtaaca tcagatggaa ttttacttaa agaaataaat ccagctttca cattagatga
    10381 tgttatttct gctactgaag ctcctttaat attgtcagat tcattaaatg gaaaaatatc
    10441 tgttgtttaa aataattaaa actagaaaat aaaaatctct tagacgtatt acatataaat
    10501 ttgtattgct taaaatatta atataaaaat aaaataaaat tggaggaaat gaaaatggtt
    10561 aaagataaag ttgtattcgt aacaggagct gcaagtggaa taggtaaaca aatagctgaa
    10621 tcgttcttaa aaaatggtgc taaagtaatg ttctctgata taaatcaaga agctttaaat
    10681 aataccataa cagagttaca aaaaaaaggt tatgattgca tgagtgtaaa gtgtgatgta
    10741 actaaggaag aagaaattaa ccacgctata gataaaactg ttgaaaagta tggcagatta
    10801 gatatattaa taaacaatgc tggacttcaa catgtttcta tgatagaaga tttcccaact
    10861 gataaatttg aatttatgat aaaaataatg ctgacagcac cttttatagc tactaaaaaa
    10921 gcatttccta taatgaaaaa acaaggcttt ggtcgtataa taaatatggc ctcaatcaat
    10981 ggtgtaatag gatttgctgg aaaggctgct tataactcag caaaacatgg acttattgga
    11041 ctaacaaaag ttgctgctct tgaagctgcc gattctggta taacagttaa tgctttatgc
    11101 cctggatatg tagatactcc acttgtcaga ggtcagttta atgacctttc aaaaactcgt
    11161 aatataccat tagaaaatgt cttagaggaa gttttgtacc cacttgtacc acaaaaacgc
    11221 ctattagatg ttcaagaaat tgccgactat gctatgttcc tagcaagtga taatgctaag
    11281 ggtattacag gacaagcttg cttactagat ggaggatata ctgcacaata gttattatat
    11341 ttagggcttg aagaaagtat tcttcaggtc cttctactaa ggagggatat ttatgagtat
    11401 tatcgcacca attggtataa ttattggtat catagctatt atatattttt cagttaaaga
    11461 aattcatata actatagctg ctccaatagc aactttaata gttgttctct taaataaaat
    11521 ggatgttgta acatcaatgc taggtgctga caaaaacaat tatatgggtg cacttggaaa
    11581 ctatataatg agttattttg caatattctt attaggctca atacttgcta aatttatgga
    11641 agaaagtgga gccactgtat caatagctga ttttatttta aataaagttg gctctaatca
    11701 tccttataga gttttagttg ctatatttat agtaagtttt atattaacgt atggtggtat
    11761 aagcttattt gtagttatgt ttgcagtcat tccacttgca agaactctat ttaaaaaact
    11821 tgatatttct tggaacctta ttcagatacc actttggctt ggtattgcaa caattactat
    11881 gactataatt cctggaacac cagccattca aaatgttata cctattcaat atctaaatac
    11941 gtctcttact gcagctacta taccaagtat tgtaggtagt ataggttgtg ctacgtttgg
    12001 acttgtctat atgaaatatg ctctaaacaa aagtattaga aaaggtgaaa cttacgcaac
    12061 ttacacttca aaattagaag aagaatcttt agatagagaa ttaccaggat tttttgcaag
    12121 tatttttcct ctattagcac tcgtaattat agctataact ggaagtactt ttggttcaga
    12181 atttatgaaa aaaaatgtaa tttacatagc tttaatagtt gcaattcttt tatctgcttt
    12241 tttattccat aaatttctat catctaagat acagacattg agcataggtg ctagtggttc
    12301 tataggtcct atatttgcta catcttctgc agttgcattt ggagcagtaa taatggctgc
    12361 acctggtttt gatgtattta gcaaacttat aatgtcaata ccaggtagtc cacttataag
    12421 tcttactgtt ttgacttcaa caatgtcagc aataacaggg tcctcttctg gtgcattagg
    12481 aatagtaatg ccaaactttg cagattatta tttaagtact ggtctaaatc cagaattaat
    12541 tcaccgtgtt gctacaatag cctcaaatat actaactata gttccacaaa gtggtgtttt
    12601 tcttacattc ttaagcttat ctaagttaac acataaaact ggatttaaag aaactttcat
    12661 tgtagttgca gttggttcac taatagcaga aattattgta attacattag gattactaat
    12721 gtattaatat aaaacaaatt aaaattcata atatgaaagg tggtgtctca aagtgaactt
    12781 tttacttaat gagacactct ttttgtatga aatcaaatat attttaatca tttcaacaat
    12841 aaaaaagaac ctttctctat tttgagacaa acccttttta ttccttaaag atttaaacaa
    12901 ttataatttg ttactaatac gaaaacatct attgaataaa tttaaccaaa taaaaaaaaa
    12961 tttatcataa ctataaattt acgttatgga attattataa aaatattatt ttctctaagt
    13021 aagtaattcc atactataca ggcttttaat acaaattttc aaacacactg aaatatacct
    13081 atttattcca tatatatctt ttttacatat ctttacagta aaaaaagtct taatcttatg
    13141 ttagtataga ttcacgggta ccccctacac taaaggttac aaatcagtaa catttttaga
    13201 atttttagat taagaggtgt ttaatgatga gaagaaatat accaacaaaa ttaaaaacta
    13261 tattagcttt aggagtgtta actttagcaa cagcttcaag tgctgttact gcaagtgctt
    13321 catcacttga aaataatgta aatataagta acaaaagttt agtatatagt aatactaaat
    13381 acaagttttc tgcaaaacca aattgttctg taaaaccaga acttaaacct gataaagata
    13441 actcttgtaa ggataagaat cataatgaca agaattgcaa caacactaat aaacctgagg
    13501 acaacaataa ttcagactct actaataagc ctgataacaa taaaccagag tctaataaac
    13561 ctgaggacaa caataattca ggctctacta ataaacctga taacaataaa ccagagtcta
    13621 ataaacctga agacaacaat aactcaggct ctactgataa acctaataac aataaaccag
    13681 agtctaataa acctgaagac aacaataatt cagactctac taacaaacct gatgataata
    13741 acaactcagg ctctacaagt gaaaattttt cagcttacca aaaagaagtt gtcgacttag
    13801 taaatataga aagagctaaa gctggattaa atcctcttac tcttgattct tctatatcaa
    13861 atgttgctac aaaaaaatct caagatatga ttgataacaa ctatttttca cacaattctc
    13921 caacttatgg ttctccattt gacatgttga aaaaatttgg aataagctat aagacagcag
    13981 gtgaaaatat agctatgggt caaaaaactc ctaaagaagt tgtaaacgca tggatgaatt
    14041 ctgaaggtca cagaaaaaat ataatgaatc caaatttcag taaaataggt gttggagtag
    14101 cacaaaaaag tggtggttca atttattgga cacaaatctt tgtaggataa aatactcttt
    14161 ttccatgcat atcaaaagag tatctctaag tgataaaaaa cacaaatgag atactctttt
    14221 gtatctattt taaaattatc aaatccaaat atacatacta taaatattat ttgttctctt
    14281 aattttagtg tgtagaacaa tatataaaaa gtattggcaa gatgaaaatg gaaaacttaa
    14341 aaagatattt aataggtaaa aacaggatct taatatcttc ataaaaaaag gctgtctcaa
    14401 aatagagata aggccttttt cattgttgaa atgattaaaa tatatttgat ttcatacaaa
    14461 aagagtgcct catgaactaa aaagctcact ttgagacact ctcttttatc aattctatat
    14521 aagcattata atactttaat attattcagc taattttttg tatccttcat atctttcttt
    14581 agcatcagct tcagctttag caaataatcc atcagcttcc tctgggaatt gtttagctat
    14641 agcagaatat cttacttgtg caagtatgaa atctttgaag cttgcagttg gttcttttga
    14701 atctaaagtg aatgggtttt taccttcagc ttttacttct ggattgaatc tgtataaatg
    14761 ccagtatccg caagcaacag cttgtgcttc attagccaca gttcttccca taccttcttt
    14821 taatccatgg tttatacatg gagcataagc tattattaga gatggcccat catgtttttc
    14881 agcttctaaa acggctttga taaattgatt tttatcagcc cccatagcaa cttgagctac
    14941 atatacgttt ccatatgtca tagccatcat tcctaaatct ttctttctag attttttacc
    15001 tgaagcagca aacttagcca tagcagcagc tggagtagct ttagaagctt gacctcctgt
    15061 atttgaatat acttctgtat caaatacaag tacgtttaca tcttcaccag atgcaagaac
    15121 atggtctaat ccaccgtaac caatatcata tgcccaaccg tctccaccta gtatccattg
    15181 agatctctta actagataat cttttctatc ttttatagct tttactaatt ctttagcttt
    15241 ttcatctgta caatcacaag ctggtttttc taataattca acaaccttag cacttgctac
    15301 tttagaagct tctccatcat ttctagaatc taaccactct gtaaatactg ctttagcatc
    15361 ttcacagata tccatagaaa ctaactctgt catagcatct actattttat ttcttatttg
    15421 cttaacagct gtgtacatac caaatccata ttcagcatta tcctcgaata aagaatttgc
    15481 ccaagatgga cctttacctt catggttaca agtataagga attgatggtg cagaagctcc
    15541 ccatatagaa gaacatccag tagcattagc tatcatcatt ctatctccat ataattgtgt
    15601 aactacttta atgtacgcag tttctccaca tccagcacat gctcctgaaa actccattaa
    15661 tggttgtcta aattgacttc ctttaacagt atttgcaggc ataacatcaa cttttggagc
    15721 tacttcatca ggatttactg caaatgccca attatctaac tctgtatcaa gttgagtatc
    15781 tattggcttc ataactaaag ctttttcttt agctggacat atgtcagcac agtttccaca
    15841 tcctgtacag tcaagtggac taacttgaat tctatatttt aatccttcta atcctttacc
    15901 taatgctttt ttagcttcaa atccttctgg agcattagct aattcttctt cagtaactaa
    15961 tactggtctt atacatgcat gaggacatat aaaagaacat tggttacatt gaatacagtt
    16021 atctattatc cattcaggaa catcaacagc tataccacgt ttttcataag ctgcagtacc
    16081 acaagggaat gttccatctt ctataccatt gaatgtactt actggtaagt tgtttccttc
    16141 ttgtgcagtc attggtctta gtatattttt tataaattct ggttctttaa cttctccttt
    16201 atctgcatca acagcatttt cccaagaagc tggaacttca actttaacta aagcatttat
    16261 tcctgcatca acagcagcat agttcatatt aactattttt tcaccttttt taccataagc
    16321 tttaactata gaatctttta agtatttaac agcatcttct tctggtatta tttcagctaa
    16381 tttaaagaat gcagattgca ttatcatgtt tattctgttt ccaagaccta tttcttgtcc
    16441 tagtttaaca gcattaactg tatagaattt tatatcattt ttagctatat attgtttcat
    16501 atgagctggt aaatgttctt caagtcctgc ttggtcccat atagtgttaa gtacaaatgt
    16561 accacctttt ttaagacctt ttaataaatc atattgataa acatatgatt gtttatgaca
    16621 ggcaatataa tcagcttcat ctattaaata agtagctctt attggagtat caccaaatct
    16681 taagtgagac atagttatac caccagattt tttagagtca taatcaaagt aagcttgagc
    16741 atatttttca gtatggtcac cgattatttt gatagcttgt ttattagctc caacagtacc
    16801 gtcagaacct aatccccaga acttacatct tatagttcct ttagaagcta tttttattgg
    16861 ctcagatgga gttaaagaag tattagttac atcatcaact atacctactg taaatccatt
    16921 tttaggttgt tctgaaacta agttatcaaa aacagttttt atatctgaag gagtagtatc
    16981 ttttgaacct aatccgtatc ttccacctat tatcatagga gcattttctt ttccatagaa
    17041 tacatcacgt acatctaagt ataatggttc accagttgaa cctggctctt tagttctatc
    17101 aagtacacat attctttcaa cagtagatgg catagcatct aagaaatgtt tcattgagaa
    17161 tggtctatat aaatgaactt taactaaacc atatttttct cccttagcat ttaagtaatc
    17221 tatagtttct tctattgttt ctgttactga acccatagct attaatacgt atttagcatc
    17281 ttctgctcca tagtaatcaa ataaactatg ttttctacct gttaaattac tcattttttc
    17341 catgtatttt tcaactatac ctactatatt ttgatagtat ttattagaag cttctctagt
    17401 ttggaaatat atatctgggt tttgagcagt accacgagca actggatgat ttggagataa
    17461 agcattatct ctaaatgctt gaactgcttc aaagtttaat aaacttgcat aatcttcatt
    17521 ttctagtaat tcaacttttt ggatttcatg tgaagttctg aatccatcaa agaaatgtat
    17581 aaatggtaat ctaccttcta ttgcagctaa atgtgcaaca ggtgctatat cagctacttc
    17641 ttgaactgaa ccagaagcta ataatacaca tccagtttgt ctagcagcca taacgtcttg
    17701 gtgatcccca aatattgata acgcttgaga tgccaaagca cgagcactta catggaatac
    17761 tcctggtaat aactctcctg caactttata catgttaggt atcattaata ataaaccttg
    17821 agatgctgta aaagtagaag ttaaagctcc tccttgtaaa gaaccgtgga atgttcctga
    17881 tgcacctgct tctgactgca tttcaacaac attaactgtt tgtccaaata tattttttct
    17941 tccttgtgat gcccactcat caactacttc agccatagtt gaagatggtg tgattggata
    18001 tatagctgct acatctgtaa acgcataggc aacatgagct gcagctgtat ttccatcaag
    18061 tgttttcata aacttagcca taatatgtat cctccttgat agctacaatt gtacttgttt
    18121 ataacaagtc tattttttta tatttacata taatttacac aacaaaaata taaacacaca
    18181 tatagtcgcg caaatactct atcttctaat ataatactac agattagata aaattccttt
    18241 gaaaattgta aattaattac atatttaagt agattttttt taaaatt
//
LOCUS       DAEPOZ010000055        18093 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.055, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000055 DAEPOZ010000000
VERSION     DAEPOZ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18093
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.055"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            50..271
                     /locus_tag="KR440_001475"
     CDS             50..271
                     /locus_tag="KR440_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698531.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
     gene            complement(560..1324)
                     /locus_tag="KR440_001476"
     CDS             complement(560..1324)
                     /locus_tag="KR440_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBH0698532.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKAPTPDIVDILSEFNVNVL"
     gene            1598..2272
                     /gene="fsa"
                     /locus_tag="KR440_001477"
     CDS             1598..2272
                     /gene="fsa"
                     /locus_tag="KR440_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBH0698533.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILDEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(2394..3788)
                     /locus_tag="KR440_001478"
     CDS             complement(2394..3788)
                     /locus_tag="KR440_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBH0698534.1"
                     /translation="MGIILKPKQFYMTVIVTIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFIFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(3866..5212)
                     /locus_tag="KR440_001479"
     CDS             complement(3866..5212)
                     /locus_tag="KR440_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0698535.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGIQQMLFSIGNMTLQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGYWKTKAIVNHRINE"
     regulatory      5462..5564
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5658..7034
                     /locus_tag="KR440_001480"
     CDS             5658..7034
                     /locus_tag="KR440_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBH0698536.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCLGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFM
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(7148..8293)
                     /locus_tag="KR440_001481"
     CDS             complement(7148..8293)
                     /locus_tag="KR440_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBH0698537.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIVLDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     SRGNRSGNTISIITKKELDIIKDLQKEFSIVITPKTLQNGELIDIIK"
     gene            8545..9252
                     /locus_tag="KR440_001482"
     CDS             8545..9252
                     /locus_tag="KR440_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0698538.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            9299..10894
                     /locus_tag="KR440_001483"
     CDS             9299..10894
                     /locus_tag="KR440_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0698539.1"
                     /translation="MSNLSILIKTNIINEFKLNALKKADSREKQKIMGMILSVVIITG
                     LLIFYVTSISLSLVDILKQINQMEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFFNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFMFIGQISSRIKYKNLILIIGSIILMLLYTLLMTRLNSMSVEMLKN
                     SVSISETIRKIYPPIYYFVDALKTGSVVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKTYKFKSQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLIIGTILILL
                     FGADKVGELLKLNIDFNFIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLGFEIQFIIVSICLIASFSMFVALMGLLS
                     NLLYPNLDWKNEMAVVKRSASMIITMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TFILDFMIWRIISTKGVRIFKNL"
     gene            10997..12079
                     /locus_tag="KR440_001484"
     CDS             10997..12079
                     /locus_tag="KR440_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBH0698540.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKEDKVNSNLDKVIEKLN"
     gene            12228..12389
                     /locus_tag="KR440_001485"
     CDS             12228..12389
                     /locus_tag="KR440_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HBH0698541.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            12730..13998
                     /locus_tag="KR440_001486"
     CDS             12730..13998
                     /locus_tag="KR440_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBH0698542.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            14193..14981
                     /locus_tag="KR440_001487"
     CDS             14193..14981
                     /locus_tag="KR440_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBH0698543.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSLRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            14981..15430
                     /locus_tag="KR440_001488"
     CDS             14981..15430
                     /locus_tag="KR440_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBH0698544.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            15434..17698
                     /locus_tag="KR440_001489"
     CDS             15434..17698
                     /locus_tag="KR440_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBH0698545.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKNALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGSMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            17838..>18093
                     /locus_tag="KR440_001490"
     CDS             17838..>18093
                     /locus_tag="KR440_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698546.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEID"
BASE COUNT         6812 a         2089 c         2879 g         6313 t
ORIGIN      
        1 gggaaaaata aatataggaa gtattatatt tacagaggag gtagttttga tggatagatt
       61 aaaaagagat atgaaaaata tgaaagaaga tttaaaagaa ggtgttaaaa agacagctta
      121 tgttatgaaa aatacagctg aagatttgaa agatgatgca caaggtgcta tggtaaactt
      181 agaaatgaaa aaagatgaga tgatggaaag atatgaacaa aagaaatttt ctaaagaatt
      241 agagaagcaa atgaagaatg aaatgaaata agcttttatt tatgaataac aaattattgt
      301 attcatataa aaagtagcca gtaatattaa atagtttatt taaggaaata gactttctaa
      361 aaataactgt attagattta gaaagtctat ttcaaattag ttttataaat agacataagg
      421 ataaagtaga tgttatagaa ctagatttta aaaaatagat actaaggata aaataaatat
      481 tatagaaata tgtttttaag aatagttatc aaataaaaat ttatatttat aagaaatcct
      541 ataaaatata taattaaact tataaaacat ttacattaaa ttctgataaa atatctacta
      601 tatcaggagt aggcgctttt tctgtaatta tagaactaat atcttctaaa tgagcaaact
      661 tgaaatttcc atcaaattta aactttttat cttccataac tataaatact tttttacttg
      721 aatttattat agccttcttc gtgagaccat cttcaatttc aaaagttgta agtgaagagt
      781 tgaatacatc tactccacaa cttcccataa aagtcttatc aaacttatat ttcgatataa
      841 actctatagt tggagtaccg acaaaaccgt ctaaactgag atttaataaa cctccagtgg
      901 aaattatatt taaattattt ggttttgaag taactacatt tagtatatcc aacatatttg
      961 taacaagtgt tactttttta ttactttcag ctaacaactt tgcaagcaag atgttaattg
     1021 tagagatatc taaaaatata gtttctctat cttcaattaa attaaatgct ttttctgcta
     1081 ttattttctt tgttggaata ttaatttctt ttctgtcttt tgtatcctga ttctctagtg
     1141 tttctctagc tagtacagct cccccataaa ttcttcttat tgaagaaatc ttttctatat
     1201 tttttaaatc ttttcttata caatcttctg taacattaaa tttagaactg agctctttga
     1261 cagtaactct accttctttt tttaggatat ttaatatagc ttggattctt tcttctgcaa
     1321 acattttcta cctcttttcg caatatagta ttatagtaaa tattataaga taaaatacaa
     1381 tatatcaact attttaacat gaaatacaaa attaatttaa tataaaaagt atgttttttc
     1441 taactatata tttagaattt agtaaataaa aaaattataa aactaagaag gaaaagcaaa
     1501 acaataataa aaaataaaaa acaataagca aaatgttgaa aataatacca aatcaatata
     1561 taataagaat aaagaaagat taaaggggga tattttaatg caactaatct tagatacagg
     1621 taatgtggag gagattaagg agttatgtac ttgtttacca atagatggtg taactacaaa
     1681 tccaagtatt gtttcaaaag aaaaaaagaa ttttaagcaa cttataaatg aaattggtga
     1741 gattataggt gaggatatgc ctatacatgc tcaagtgtta agtacaaagt atgaagaaat
     1801 attagacgaa gcattatata taagtagttt gagaaaaaat atatatgtaa aaatacctgt
     1861 aacacaagat ggtttaagag ctataaaaga ccttcacaga aaaggtgtaa aaataactgc
     1921 tactgcaata ttcactgctc atcaaggttt tttagctgca aaggctggag caaattatat
     1981 tgcaccttat gtaaataggc ttgataatat atctggtgat ggagtagcta tggtttcaga
     2041 acttataaaa ataatagata cttataaaat ggatacaaaa gtacttgcag ccagttttaa
     2101 aaatgcacaa caagttatag aattaatgca acatggggtg catagtgtta cagtaccata
     2161 tgatatttgt aaatctatga tgaatcatcc attaactgat tggagtgtag ataagtttat
     2221 agaggattgg gagaatactt ttggaaaagg aagtaaaaca aataatattt agtattatgg
     2281 taaaagagta gttttgtcca atttcacaag aacttatata aaaagatgaa aatatgaatt
     2341 ttctttttaa taatttcgtt attatcttaa gttaattcat attttcatct tttttagatt
     2401 tctagtccta taaatttcca ccaagggaat gttatacata ttattataaa gtaggttaaa
     2461 ggaatcaaag ttaaaccgta tttgaaaata accttgctat tatacagatt ttcaccatga
     2521 ccaaccatta ttgtagcatg atgaaatggt aatatgtact gtatgcttac tataatgtag
     2581 acaaatagac ataaagtaac actattttcc tttagtatat gtaaacttcc aagtaatggt
     2641 attactacag atgatgtagt gacagcactt ccaagacata tattcaagat tattgtgaga
     2701 gttatcaaaa acaatattga aataatttct gaagagtttg gtataaactt tatcaaataa
     2761 tttcccatta tatctacaac tccagagtac tttaacactc ctccaattga aaatgctgct
     2821 ataaaaaaaa taaggacctt ccaatttatt tcttttaaag tactaaatcc aactattttt
     2881 cttagtaata aaagaagtac acaaattgac attataatta atgtgtttat gttatgtatg
     2941 aactgagtta aaaacataaa aaacattatt cctaggcata aaaatacgtg tttttgctct
     3001 ttgtttagca aattaaattc tttttttaaa ttagaatatg aaaaatgaag tttattaccc
     3061 tcaagatttt cattagaaat aaattttatt gaatgcaatt cttttttaaa gacaaagata
     3121 aataaaaaat aagttataaa acaagtaatt atagttggaa cagacatata aaaagcccat
     3181 tgaccccaat ttatattaca atttccaagt tctagtacaa cataatttag aagggtgtct
     3241 ccatttacaa aaaacattga tgtaaaggta gatgctgtaa atatactaaa taaaagtata
     3301 gattttgaat cttcagttat ctgttgttct tttaaaaact ccttgtaaaa agcagataca
     3361 agaataaccc ttggaaatgg ttgtgggata aaaaatatta aaagaatacc taaaatatac
     3421 gataaaagaa tcatcttttt aggggtattt acagtattta aaattaactt ctcagaaata
     3481 atttttgcga cacctgtttt tgtaactgca ttagtaatta tgtatgcaag aattattatg
     3541 taaaaatttt ctgtaaataa aaactgtaaa ataaccttta ttggtgcaat gttaaatata
     3601 aacgctagaa ttacaaaaaa caatgcaaca aaagtttttt caactgagtc tacagcccat
     3661 atcaagatag ttaaaaaaga taagcatata gaaaatttta tgttgaaact gtaattaaga
     3721 gagaagtaga gaattatggt aactataaca gtcatataaa attgttttgg cttaagaata
     3781 atacccattt ttatattcac ctccataatt tattatatca taatggaggt gaatacaatg
     3841 aatgtgaata cattttataa aaagattatt cattgattct atgatttaca atagctttag
     3901 ttttccaata ccctttctta tagtagataa ctgtaacaat aagacctaaa gtccaaccaa
     3961 aaggaatacc catccaaatt ccgtctgttc caaaaaattt agaaagcata taagaagctg
     4021 gaagtcttcc aagccataaa gatactattg agcttataag tgcaaacatg gaatcaccag
     4081 cacctttcat tgcacttgaa agtacaaaag aagttccaat aaataaaaag aatggtccta
     4141 ttataaataa gtaagaagag ccaattttaa tgacatcttt atcagtattg aatagagcaa
     4201 ttagattttc cctaaatagc aaaattaaag caataactgt aactgctaaa actaaggtca
     4261 tttttataga ttctcgaatg ccttttttaa ccctttcatt ttctccagca cctatgtttt
     4321 gagcaacaaa agtagaaaca gcagaaccaa gattcataat aggtaagctt ataaaatttt
     4381 ctatcctaag accagcacca aaagctgcca tagcagatgt accataacta ttaactaaaa
     4441 attgaagagt catatttcca attgaaaaca acatttgttg aattccactt ggaagtccta
     4501 atctaagtcc atctttaaat attttgttat cgtatacaat tcctttgatt ttaaatttaa
     4561 gcacttcatg ttttttattt aaataaatta tacttattat aaatgatact gcctgagcca
     4621 ttatagtggc aagtgcaacg ccctcaacac ccattcttag cactacaata aaagttaagt
     4681 ctaaaactat atttagaatt gtggctatta tcagaaaata aagtggagtt actgaatctc
     4741 caagtcctct aagtattgca cttactgaat tatatccagc agaaaataat attccactaa
     4801 atattatagt aagatacaat tttgattgag caaaaacgga ttctggtgta ttcataagct
     4861 taagcattgg gccaccaaat actattccaa gaatacttat taatatagaa ccaataaata
     4921 aaagtatata tgtagtatca atagtagctt ttaccttttt taaatctcct gagccataaa
     4981 attgtgacac taaaatagaa aatccaaggc taacacccat aagcgctgcc accaataaaa
     5041 acattatagg gttagctcca gagactgccg ccattgcttc ttttccgata aatctaccaa
     5101 ctattatact gtcagctgta ttgtatagtt gttgaaataa actaccaata agcataggca
     5161 tggcaaaaaa gaatatggat tttccctcat gacctgtagt taaatctttc accttattcc
     5221 tccaaataaa aaaattatta atttattata gtataatctt aacataaact ttatatccaa
     5281 actataaata aattgtatca aaatatcata tattttttta aaatgtaaag aatataatta
     5341 taatttgaat ttaataaatc aatatttaat ataatcgaat attaatacaa aatcaataca
     5401 atatatacgt atatattgac aaaattaatt atataaatta aaataatatt cgtaaagaaa
     5461 aattcatgtt ttgtactgta taacatataa ttttgacaat atgggtcata agtttctacc
     5521 ggaataccgt aaatattctg actatgtata ctattattta gttaattaaa tatttaatta
     5581 actgaattat gaaggatacg gctcagctta tcaactactg tgtcgttttt ttgtaaaatt
     5641 taataggagg tgttgttatg caaacaacaa acagtagtac tatgcagaaa ctttttccaa
     5701 ttttgactaa taaaaatgtc gacatgaaga aggaaataat tgcaggagta actacttttt
     5761 taactatggc ttatataata gctgtaaatc caaatatttt atctgagaca ggcatgcctg
     5821 caggtgcact tgttacaggt acttgtttag cagcagcatt tggatgtatt ttaatgggtg
     5881 tagttgctaa ccttcctttt gctttagcat caggtatggg tcttaatgca ttttttgcat
     5941 atacagtagt tttacaaatg ggtgttcctt gggaagtagc tcttacagca gtatttgtag
     6001 aaggtattat ttttatagta ttatcagtaa gtggagttcg tgaggctgtt gtaaatgcga
     6061 taccaaaaaa tatgaaatta gctgttacag gtggtatagg aatatttatt gcacttatag
     6121 gtctagttaa cagtggaatt gttattggag accaagccac tcttatcaaa atgggtagat
     6181 ttacaccagc agtaataata acttgtttag gattaataat tattgctgta ttagataaaa
     6241 aaagagttaa aggttctata ctttttggaa tagtagtgag ttcattactt gcttgggcat
     6301 ttgcttttat gaatccagag tatgcacaaa aattaggtat atatttacct ggtggattat
     6361 ttaaattcga aagtttagca ccaatagcag gtaaaataga tttaggatat gtactacatc
     6421 caacaaatat aggtggtttt ttagtagtag tttgtacatt tttatttgtt gatttttttg
     6481 atacagtagg tacattagtt ggtgtttgtt ctaaagcaaa catgttagat gaaaaaggga
     6541 atgttcctaa tgtaggtaga gccttactta cagatgcggt tgcaactaca gttggtgcag
     6601 gacttggagt atctacagtt acaacttatg ttgagagttc aacaggagtt atcgcaggtg
     6661 gaagaactgg atggacagca ataacagttg gaattttatt cttagctgca atgtttttct
     6721 ctcctgtatt tatagcaatt ccatcatgtg ctacagcacc agctttaata tatgttggat
     6781 atttaatgct tggtacagtt aaggatatag agtttgataa tataactgaa ggtgttccag
     6841 cttttgttac tattgcttgt atggcattaa catatagtat aggtgatggt ttaactttag
     6901 gtatattaac ttatgttttc gtaaatatat tttataatat atttggagct aaaaaggcag
     6961 aagacaaaaa acatgtttct gtagtaatga ttgttctggc aatattattt ataataaaac
     7021 tttggctatt ataataggta ttatttaaaa ttttaattta atataaaata aagggctata
     7081 atttattatc aattgtgatg ataaactaca gctcttttgt atttgttgta ttttcgtatt
     7141 tattgtatta tttaataatg tctattagtt ctccattttg taaagtttta ggtgtaatta
     7201 ctatactgaa ctctttttgc aaatctttta taatatctaa ttcttttttt gttattattg
     7261 agattgtgtt accacttcga tttcctctag aagttcgacc acatctatgc aggtattcat
     7321 ttttactttt tggaaaatcc aagttaaaaa catgacttac atccacaata tctagtccac
     7381 gagcagataa atcagttgta attaaaatct ttgctttacc aagtttaaat ttatttattg
     7441 cattctttct atcttctttt tccatatttc caaaaattcc tattgctttg taattatgat
     7501 aatttagttt tgatactagg acttctatat ttttttcatt atttacaaat acaatagctt
     7561 tttttggatt tgtagctgct aatgcctttc taagaaaagt aaatttatcc cttatctcac
     7621 ctaataaata tgagtgattg atgttaggat ttatttgtga tttttccttt gtttttatta
     7681 tttcaaactc cttcataagt ttatcacaaa tttttatagt gctatcagta agacttgcag
     7741 agcatcctat aatttgtcta tctcttaacg ttgttcttat tatgtcttct atacaagtta
     7801 tattttttcc attcaataga ttgtccactt catctaagac tatagttttt atattatgtg
     7861 attttagctt cttttgtttt atcaagtcta aaactcttcc acaagagcca attactatgt
     7921 gtggttttac tgcttttata ttttttattt gtttttgtat attgacctcc ccaattaaag
     7981 ctaaagaagt aacggagagt tctgcatttt tagccaataa ttcaacctgg tttgtaatct
     8041 gcattactaa ttcatgcgta ggtgcaagta taagagcttg tgtttctctt tttgatgtat
     8101 caattttttc aaatataggt aaaaggtacg ctagtgtttt tcctgttcca gtctgtgaat
     8161 taatcaataa atccttattt tgaattatat tccctataac aagtgactga acttctgttg
     8221 gagaagttat gtcttgtttt ttaagtccat ctattagagt ggaacttatt tttaattgtt
     8281 caaatgtatt catattttgc tccttatatt taataattat agtcaactat tttttattat
     8341 acattgttta ttcaaatcga actatatatt cttttataat tatgtgtaat ttgaaagtaa
     8401 atatcaatta tatttatacc tttatatttc tgataccttt gcattttata gatttctaaa
     8461 ataaacaaat aaaagttgta attgtttgat atttgttata tattatatat aacaaatatc
     8521 aaaactataa tatggaggga aattatgtta aaagtaatca atgtatctaa atcatataaa
     8581 aataaaaaag tagtaagtag tgtctcattt gatgtaaatg atggagagct ttttgggttt
     8641 ataggacata atggagcagg taaaactact actattaaag caattgttgg aatacacgat
     8701 tttgaagagg gagaagtact aataaattct aaatctataa aaaaacagcc tgttgaatgt
     8761 aaaaaggaaa tggcatatat acctgataat ccagatttat acgagtcatt aacgggtata
     8821 caatacctta attttatagc tgacatattt gaagttgcca aagacaaaag agaagagtta
     8881 ataaaatatt attcaaataa gtttgaactc aataaagcat taggagattt aatatcttca
     8941 tattcgcatg gtatgaaaca aaaattagct ataatttcgg ctttgataca cagcccaaaa
     9001 atcttaattc ttgatgaacc ttttgtaggt cttgacccaa aagcttcctt tatattgaag
     9061 gaaattatga gggagttttg tgacaaaggt ggttgtatat ttttctcaac acacgtttta
     9121 gaagttgctg aaaaaatttg tgataagatt gccataataa aagatggaaa tataattgct
     9181 tatggaacta cagaagaagt taagggaagt aattcacttg aaagtatctt tatggaatta
     9241 atagagaaat aaaatgtttt ttatacaata tagtgtctaa agtgaggaat gataaaatat
     9301 gagcaatcta agtatattaa taaaaactaa tataataaat gaatttaaat taaatgcgtt
     9361 aaaaaaagct gatagtagag aaaaacaaaa aataatgggg atgatacttt cagttgttat
     9421 tataacaggg ttattgattt tttatgtaac tagtataagt ctttctttag ttgatatttt
     9481 aaagcaaata aatcagatgg aaatgctact tatatttgga tttggattat caacattaac
     9541 aactattgtt acatcattat ataaaacgtc atcatatctg tttcaagcaa aagatttaga
     9601 ccttttgact agtttaccta taaaagaatc tactgtacta gcaagtaaga tattaatgtt
     9661 ggttggagca aactatttat tttcttcaat atgtatgatt ataccagcag tggtttattt
     9721 ttttaattct gatgtaagta taacgtattt cccttatttg atactgctat ttttagcttt
     9781 accattatta ccaatagttg cttcatcaat aatttttatg tttataggac agatatcatc
     9841 aagaataaaa tacaaaaatt taatattaat tataggtagt attatattga tgttgcttta
     9901 tactctactt atgacaagac ttaactctat gtcggttgag atgttaaaga atagtgtatc
     9961 tatttcagag acgattagaa aaatatatcc acctatatat tattttgttg acgctcttaa
    10021 aacaggaagt gtagtttcct ttttagcatt tatagcaatt tcaataatac cattttttat
    10081 gtttgtaaca ttatttgcaa aaaaatttaa agatataaat gctaagatga gtgaaggata
    10141 taagtcaaaa acttataaat ttaagagcca aaaaacttca agaccttcaa aagctctatt
    10201 aaaaaaagaa tttaagagat atacttcttc atttatatac tttttaaatt catctgcagg
    10261 tttgttattc ctaataatag gtacaatttt aatattatta tttggagcag ataaagtggg
    10321 tgagttactg aaattgaata tagattttaa ttttataaaa atacaattat tgggagtatt
    10381 cttctttata atagtaatga attgtacaac atactgttca atttctcttg aaggaaaaaa
    10441 tttatggata ctgaaatctt ctccaataga tgaaagagaa atattctggt caaaaatatt
    10501 gatgaatttt ttattgaatg caccattatc tattttatgt ttaatattag tatctataag
    10561 attaggtttt gaaattcagt ttattattgt atcaatctgt ttgattgcaa gtttttcaat
    10621 gtttgtggca ttgatgggat tattatcaaa tttattatat ccaaaccttg attggaaaaa
    10681 tgagatggct gtagtaaaaa gaagtgcaag tatgataatt acaatgttag tatcattagc
    10741 ttacgtaatt atacttggag gtatatatgt attacttgga atttcgtttt taaatatata
    10801 tattattttg gcaagtatac ttacttttat attagatttt atgatatgga gaattatttc
    10861 tactaaagga gtaagaatat ttaaaaattt ataggtgtat ttatgtcaaa taaagactta
    10921 tcttttttag tcttgcaaat aataggttaa gtagtatact atgtatagtg atatgaaaaa
    10981 atttaggaga aaataaatga gaaaattatc caaaagagaa aaagatttta ttcaatatgg
    11041 atttttatta tttttgatat gtattactac ttatttagta ttaactactt tagatataaa
    11101 actaatacct aaaattataa ggctagtaaa taagacttat atatttcttg gcatattgac
    11161 aattatagta tatatgataa ttgaatctat tgtaactcat ttgataataa atgcagttca
    11221 aaaaacaaaa gtaaaattta ttggtattaa aatggctaca atgggatttt actataactt
    11281 agttactcca tttgcatctg ggagtcaacc aatgcaaata tatgcactta caaaatatga
    11341 tgtaagcctt agtaaatcag tggctatagt tactaataaa actgtagtat ttcaaagtac
    11401 agttactgta ttttgtggaa ttttaatttt gctaaataag tcattactga tggagcaagt
    11461 acattctgtg aaagtcttga taacttttgg aatgattatg aatgtaagta tgttgttagg
    11521 tggactatta atagtattta gtccaaaaca agtaaaaatg atagcagaag ttttgattaa
    11581 ttttttatct aaatttaaaa catttaaatt tttagattca aaacgtgata gaataaatca
    11641 ttatattgat gattataatt attctataaa aatatttatt aaaaatgtaa gaatcttagt
    11701 tttaagtata ttacttactg tgttacaatt aatcgcatat ttcagtatag tttattgtgt
    11761 gtataaagcc tcaaatttaa gaggagtttc atatgtacat ataatgacat tacaggtctt
    11821 tttatatatg gctatatcac caattccaac accaggaaat gttggagcta atgaggttac
    11881 attttttact atgttctcca atataattcc aaaagaatta ttggggtact ctgtactctt
    11941 atatagtggg tttgtatact attttatatt aatagtttca ggtttattta catttggaac
    12001 gcattataca ttaaattctt ggaaaaagga agataaagta aattctaatt tagataaggt
    12061 tattgaaaaa ttaaattaat aaaataatta gggaaattct atatataaat atggtgttga
    12121 gtaagctata ttatattgag atatttccct tttttgatta aaattataga aatttgaaat
    12181 aataagtaat aaacagaaaa agtgatattg ttttgaaagg agaaactatg aaacaagata
    12241 aacaaaatga aaaacaaagt gagatttcta agataaaaca aagcaatgat aataaacttg
    12301 aaatagctca agaattacat gtaagtaaaa atatagggaa tatgcttgga tatagaatag
    12361 ataagaaaaa aatgaataga ccaagttaat gaaaatgttg aaaatggtac attttaaaaa
    12421 atatatataa tgaaatgttt tattgttaaa tttaaactta ttttaatgga ggaaaaagtc
    12481 gaaaaatatc taaaaagata tggatataaa aaatattaat atatatatac tataaacttg
    12541 acaaaatcaa ccttttaata actagcaaaa aatagcaaaa ttatataagc gttatttaat
    12601 tatcaaaaat gaaagctaaa ttgcatgtcc taaaaaaaat gattgataac atttatttgt
    12661 gtgctataat tatatagtaa aacaataagt atattgcata atgtatgcaa atcgggagga
    12721 gagacggaca tggaagcatc tatacacaca attgccagca ataatttatt gataattttt
    12781 gctattgtaa gtataactgg aataatttgt agtaagctta gtgaaataat aaaggttcca
    12841 gatgtagtgt tatatttact tgtaggtatt ttaataggac catcattttt aaaatttata
    12901 gatattcgtg gatttcaaat agaaaatcaa ctaatactta catttggttc tgcatttatt
    12961 ttatatttag gtggaaaaga aataagttta aaagtattga gaaatgtgaa gatatctgta
    13021 ttcttattag ctactttagg agttgtgata tctgctttta taatgcaaca agtaatagga
    13081 tttacttttg ggataagtgc aatgactgct ttactagcag gaactataat agcatcaaca
    13141 gacccagcca ctcttgtacc tatatttaat caggtaaaga taaaggatag ggtaaaacaa
    13201 actgtaataa gtgaatcagc ttttaatgat gcaacaggag ctatactaac atcagcagtg
    13261 ttggctgtaa ttttgtcaaa taaattttca ttaggtgaaa atatttatga actggggaca
    13321 atggttattg tcggagtttt agttggttta attacaggaa tactgctttt gaaattagtt
    13381 aatgataaac cttatggaat atttaaagat tttgcaccaa taatatctgt tatttcagtt
    13441 gtagttgcat atgaattatc tacaaaatta ggtggaagtg gatatatgtc ttgctttatt
    13501 gtaggtataa taactggtaa caagaaaaac tttaaaattt ggttaagtca aaaatcttat
    13561 gatgcagatt tttatgttgc tgaaacttta ggtactattt gtcgtatggc aatatttata
    13621 atattgggta gccaagttaa tttggctcag ttaagtaaat attttatgcc atctgtaatt
    13681 acagtattag tattaatgtt tatagcaaga ccactttgcg ttttagtttg tacacttgta
    13741 gacagggaag caaaatggag taaaagtgaa atattattta tgatgtgggt aagggaaaca
    13801 ggcgtaatac cagcagcact ttgtggtatt atttcagcaa tgaaagttcc tggatacgaa
    13861 gtgatttcat ctgtagtatt tatgactata ttaattacat tagtaataca aggaagtact
    13921 acaaaattag tagctaaaaa acttggatta ttagaagaag aagtgataaa tataagtgaa
    13981 aaagtatcaa ctttttaaag ttttgcttta aaatagtacc tgttaatggc tattttaata
    14041 ttgtctaatg atgattattg aaatagtcat ttttttatgt ataagtaatt tacagaattt
    14101 acatattaga catatttatt atcaaaattt gttgaagcta tgatataatt agttgtattt
    14161 aaaataagaa tagcaaataa gggaggaaat ttatgtttac ggttatggat attgtacagc
    14221 cagatacagt agaagaagcg tactcaattt taaataaaag aaaaactaac caagtaattg
    14281 gtggaagtgc ttttttaaga atggggaaaa aaagaatagg aacaggaata gaattatcta
    14341 aattaaattt agattatata aaagagtatg aagattacgt tgagattggt tctatgacta
    14401 cttttagaac attagaaaca agttccataa taaaaaataa ttttggaaga ataatagaag
    14461 attcagttaa aaatataata ggagttcagt ttagaaatgt agtaacagtt ggtgcaacag
    14521 tattttctaa atatggtttt tctgacttga tagtagcatt actttcatta gatacggaag
    14581 tagaattata taatattggg agaattagtc ttgaagagtt tctaaataga gattatgaga
    14641 aagatttact tattaagata tacataaaga agacaaataa gaatgcttct tataaatcac
    14701 ttagaaatgc aaagagtgat tatccaattc taaatgtatc tgtatcaaaa catatggaac
    14761 aatttaagtt atgtgtagga gcaagaccgc agaaagctac gattgcaaaa caagctagtg
    14821 aatttctatc taacaatgaa atcaatgaaa ttaatataga taaagcagta gaaatagcat
    14881 cagaagaact aacttttggt tctaacatga gagcatctag ggagtataga aaagctatga
    14941 gtaaagtact tttaaaaaga gctataatgg aggttattta atgttagtaa agattaatat
    15001 aaatgggaag aaaaaaagtg ttgatataga accagaagaa tatctggttg atactttaag
    15061 aaaagttggt aatttaagtg tgaaaagagg ttgtgacaca ggctgttgtg gtttatgtac
    15121 agttttaatt gacaagaaac ctacattatc ctgtgcaaca ctgacattga gagctttaga
    15181 taaagaaata actacaattg aaggtttaga aaatgaagtt aaagaattta gtgaagtttt
    15241 ggtaaaagaa ggagctgagc aatgtggttt ctgttctcct ggctttatac ttactgtact
    15301 tgcaatgaag gatgagcttg aaaatcctac agatgatgaa ataaagcatt atcttacagg
    15361 aaatctatgt agatgtacag ggtatatggg acagttaaga gctatcaaaa cttatctgga
    15421 ggtaaaatag aacatgaata tagttggaaa aggaatcaag aaaatagatg gaatgagtat
    15481 cacaacagga aagcctgtat atacagatga tttatcagca aaaaatgctt tggtggtaaa
    15541 gatacttaga agtcctcacg cttatgcaaa aataaaagat atagatacat caaaggcaga
    15601 aatggttgat ggtgttgaat gtgttttaac atataaagat gtaccaaaat ctcgatttac
    15661 acttgctggt caaacatatc cagagccatc tccatatgat agattaattc ttgaagatgt
    15721 tgtaagatat gtaggtgacg aagtagctat agtggcagct atagatgaaa aaactgcaat
    15781 taaagcaatg aatcttatca aagtaaagta tgaagtactt acacctgttt tggattttga
    15841 agaggcaata ggaaataaaa ctatggtaca tgatgaagat agacattgta actttgatat
    15901 aggtatgcaa agagaaaaaa atatagtatc ccatcatgat tatacaaaag gtgatgtaga
    15961 aaaagtatta agtaattgtg atgtagtgat tgaagaaact tattatactc aagctcaagc
    16021 acatgcaatg atggagacgt atagggcata caattattta gaccatatgg gaagattggt
    16081 tgtagtaagt tcaactcaaa taccatttca tgtaaaaaga catttatctc gtgcactaga
    16141 aataccagct agcaaaatta gggttataaa acctagaatt ggtggcggat ttggtggtaa
    16201 acaaactgcg tgtgttgaga ttttttctgc aattgttact ttaaaaacag ggaaaccagc
    16261 taaaataatt tatgatagaa aagaaacttt taattgttca acaagtagac atgcaatgag
    16321 attaaaagtt agactaggtg ctacaaatga tggcataata aaggcaatag atataaaagc
    16381 actttctgat acaggagcat atggagagca tgcttctaca actattggtt tggttggaga
    16441 aaaaacttta ccaatgtata ataaagttga agcaacaaga tttattgcag atgtggtata
    16501 tactaataag atgccagcag gagcacttag agggtatgga gcatcacaag gatgttttgc
    16561 tgttgaatca actataaata tgcttgctaa taaattaaaa atggacccaa cagatttaag
    16621 gttaaaaaat gtggtaacag aaggtgaaac tacatttgca tatggtaaaa ctttaaatag
    16681 tgtcgattta ataaaatgta taaataaagg caaagaactt attggatgga atgaaaaata
    16741 tccttgtaaa catcttgata atggaaaaat tagaagtgtg ggtatggcaa ttactatgca
    16801 aggttctggt atacaaggtc ttgataagtc ttcagctgaa ataaaattga atgactgtgg
    16861 agattatact cttatgatag gttctactga tatgggtaca ggaagtgata cgattttagc
    16921 tcaaatggct gctgaggttc ttgatacaag cattgaaaat ataacagtta tatcagctga
    16981 tactgactta gttccatatg acccaggttc atatgcttca agtactactt atgtaacagg
    17041 tatggcagta gttaaggcag ctcaagactt aagagaaaaa ataatttctg ctggagcaac
    17101 taaactaggt gttgagaagg atgaagtaga atttgatggt ataaaattaa ctagtaaaaa
    17161 aggtagcact ttattattcg atatagcagt tgattctaca attggtccag atactaatca
    17221 attaataggt cttgcttctc atggtagccc agtatctcct ccacctttta tagcaggttt
    17281 tgcagaaact gaaattgaca cagaaactgg aaaagttgat ttagtaaatt atgtagctgt
    17341 agtagattgt ggaacggtta taaataaaaa tctagctaag atacaagttg aaggtggaat
    17401 tgttcaaggg attggaatgg cattatttga agaagttaga tacacagaaa aaggaagcat
    17461 ggacacaaat acatttatgc aatacaagat tccagctaga tgtgatattg gaaatgttaa
    17521 agtagatttt gttgaaactt atgaaccaac tggaccattt ggtgctaaat ctgttggaga
    17581 agttgttata aatacacctt taccagcaat acaagaagct gtgtttaatg gaattggtgt
    17641 aagaataaat acactcccta ttacacctga aaaagttttt atggaaatga ataaataatt
    17701 atttatgaaa atttagacta gttttacatg ttaaacaacc agttatgtca tacctgttga
    17761 aagatattta ataagtagtt attataatat tatgaatttt acttataaaa tacaaaatta
    17821 aataagggga tgatgttttg gctggtaatc taaataatat gagagcagta aataatttta
    17881 gaggagataa gaacatttta gaatgtttag tcagctttga gggtcgttca ataagtcaga
    17941 gaaaagtaag ggtatttttt aaagaaaaac aaaatcaaat agaaattgat tttgcagaag
    18001 aggaaatttc taaattggtt gaaaatgttg ttttaaatac atcatatcaa gaaatgttat
    18061 atgatgaaat agagaaacaa ctggaaattg att
//
LOCUS       DAEPOZ010000056        18065 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.056, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000056 DAEPOZ010000000
VERSION     DAEPOZ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18065
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.056"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..1497)
                     /locus_tag="KR440_001491"
     CDS             complement(<1..1497)
                     /locus_tag="KR440_001491"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBH0698547.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNLENEKVREECYKVLEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQ"
     gene            complement(1694..2416)
                     /locus_tag="KR440_001492"
     CDS             complement(1694..2416)
                     /locus_tag="KR440_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0698548.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECIGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            2807..4234
                     /locus_tag="KR440_001493"
     CDS             2807..4234
                     /locus_tag="KR440_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH0698549.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNVDYLFLLGAVSAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGARLGSFNGASIIDYVLSFSYGDKAW
                     LVIPVGVVFFVLYYVTFKYIIIKDNVQTPGHEVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            4227..5006
                     /locus_tag="KR440_001494"
     CDS             4227..5006
                     /locus_tag="KR440_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HBH0698550.1"
                     /translation="MHKMKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPF
                     SRQAIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGK
                     EVFNYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPY
                     YLDSCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSS
                     LIQERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            5269..5994
                     /locus_tag="KR440_001495"
     CDS             5269..5994
                     /locus_tag="KR440_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH0698551.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNEDKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEAAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            6124..8049
                     /gene="mngA"
                     /locus_tag="KR440_001496"
     CDS             6124..8049
                     /gene="mngA"
                     /locus_tag="KR440_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBH0698552.1"
                     /translation="MEFKKITNENLVFLKQKIKTKEDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIENWESVDSNNKVELI
                     FLIAVPESEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIVLAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKKADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVIGKPVAA
                     LNNGLINWLNGMTGINAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIHVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AILLIITRKQKNQRKIH"
     gene            8193..10808
                     /gene="mngB"
                     /locus_tag="KR440_001497"
     CDS             8193..10808
                     /gene="mngB"
                     /locus_tag="KR440_001497"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBH0698553.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPERKFFLSRYENIFKELEKNKDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHQEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLVAFNLMP
                     YNRNEIVNAQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKISDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIINVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEEWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGKKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKIPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKEEALVVRFYNSKEETKSANVVFNKNIKTAKWTNLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            11090..12244
                     /locus_tag="KR440_001498"
     CDS             11090..12244
                     /locus_tag="KR440_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBH0698554.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDENNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEELDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLNSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(12367..14280)
                     /locus_tag="KR440_001499"
     CDS             complement(12367..14280)
                     /locus_tag="KR440_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0698555.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     STGEFILSSKKGYKVNLNNNCQTKVDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     ELFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLIGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IESVEVVNYIDNNNDISISRDISNFLESEYNLTLTSAELYYLVFQLTNKTTVLNYHEM
                     DTKSLPNYIDEHFVKLTKKIIKNVYDLYFIDLSDDEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQILENIEIDEDEISYISLHVGSFFDRQKLLED
                     KILCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIS
                     DRCPIPFVYVNPYLNRKDYDNIQSKFIQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIKFMGNILYKNKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            14528..14845
                     /locus_tag="KR440_001500"
     CDS             14528..14845
                     /locus_tag="KR440_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0698556.1"
                     /translation="MKNIKVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLPEIDCLLLGPHLDYMKDEIQKKADEYNVPVACISKTAYGRLDGVKALEEALELI
                     LNK"
     gene            14853..16100
                     /locus_tag="KR440_001501"
     CDS             14853..16100
                     /locus_tag="KR440_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0698557.1"
                     /translation="MGRLFMNKFIDLLSEKFTPVVNKITKNIWVQSVQSTIMKVLPMV
                     FVGSLVTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMEIMKNKKFESMSVVA
                     GLAGAGLMLMMIRPEITNEGAIFNFNRFGGEGMLVALVAGLFSGLIMSLFGSFSFFGE
                     DSALPDFVSKWFDSMLPIAVIMFIGWGLIYGLNFDMFEIIVRIFKPVSDIAQTLPGFV
                     LLNFLVVFLYSLGISGWVLSPITYAIQLSAIAANAAAVASGGLATNIYTYEVVQTGWI
                     TLGGWGATLPLVLMMCLSRAGRIKAIGRACIVPSIMNINEPVMFGAPVAWNPFLMLPM
                     WIGTVVISITTYLSMSMGFVTIPSKVFNLWYLPFGISTFMVNQDVRGLIVLAINIVLL
                     FMIWYPFFKAYEKEELKLESQEA"
     gene            16129..16482
                     /locus_tag="KR440_001502"
     CDS             16129..16482
                     /locus_tag="KR440_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBH0698558.1"
                     /translation="MTTEEHVIDEELVEVAMQIILRAGEARTEIKHALNDLERFDYKN
                     ADLKLAKAKEFMTEAHRAQTNIIQGEASGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            16504..17961
                     /locus_tag="KR440_001503"
     CDS             16504..17961
                     /locus_tag="KR440_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBH0698559.1"
                     /translation="MIKLSKFPKDFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKHYESDMTVDKIKFAMKDNNGYYPKRNGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKDKVKYWIVINQINLMYHESFNSVAFCVDQVDDVEEAKYQ
                     AIHHQFMATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRIQYFFT
                     DVSFRGEYPAYIKRFFKENNINIVMEEGDDKVLKENTMDFLALSYYYSTTVSASKNTM
                     NPIDNVKNQYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKERYKKDSFYWYKNVIRTNGEELKEKVDSF"
BASE COUNT         6879 a         2090 c         3108 g         5988 t
ORIGIN      
        1 ttggttttta tactctcttt cataaacaaa tagattttta tgattttcat ctaacaatct
       61 ataagtacct tctgtaaaaa tatcatattg tttacgatat ttgattaatc tttgataagt
      121 ataaaatact gaacgagaat cattaagctc attttctaca ctaatattgt cattattaac
      181 ttttaaccac ggtttatgat tagaaaaccc cttataaata gtattatccc attgcatagg
      241 agtacgggca ttatctctgg atttttgttg aagtataagc aatgcctcct tttcatcaat
      301 accagattta atcatatgat agtatgcatt cttactttca gcatcatcat attcatctat
      361 tttagtaaag taggcatttg tcatacctat ttcttcacct tgatatatgt atgtagtacc
      421 ttgtagacca aatagtgtta tggctaacat cttagcactt tcttgacgaa aaaatctatc
      481 atctccaaat cgagataaag ctctaggttg gtcgtgatta gagtaaaata gagcattcca
      541 tccctttcct tcatacattt tatattgaaa gtcagattgt gcttttttta atttatccag
      601 agagaacatt tcattagtcc attttgcgcc tcctttataa tctgttttca tatgatggaa
      661 gctaaaaacc atagatagtt cttctcgatt ttctctagta tagttgatac actcttcagg
      721 tgtagtagat gacatttcac ctacagtaac tatattttta ggtttgaaaa ctttttgatt
      781 catattatgt aaatattcgt gtatacgagg accatcagcg taatgagttc ttccatctcc
      841 ttttggccct gttatagggt catcaggtaa tccaggtgtt ttggagagta ggttgactac
      901 atctaaacga aaaccatcaa tacctttatc agcccaaaat tctagtactt tgtaacattc
      961 ttcacgaacc ttttcatttt ccaaatttaa atcagcttga gtttcttcaa ataaatgtaa
     1021 ataatattgg ttagtagttt catcatattt ccaagcagtt ccactaaatc tggatatcca
     1081 gttattagga acagaaccat caggtttagc atctttccag aaataataat cacgatatgg
     1141 attgtctttg ctttttttag attctttaaa ccatttatgt tcagtagatg tatggtttag
     1201 gaccatatcc atcattattt ttatgtctct tttatgagct tcttttaaga gtttttcaaa
     1261 gtcactcatt gtcccatatt ttgggtctat attataataa tcttctatat catatccatt
     1321 atctctttga ggagaaacgt acataggagt caaccataat aaatcaacac ctaaagaata
     1381 cagataatct aatttttcta taataccatt tatatcacct ataccatcat tgttgctatc
     1441 tttgaaactt tttggatata tttgatagac agttgctttt ttccaccaat ttttcataat
     1501 tgatacctcc tgtaatttat aagttaatta taatatgttt aaacaaattg tcaagtatta
     1561 aactaatatt aaagctaaaa tatacaataa ataattattt gtataaacat ataattgtat
     1621 atagaatagt gaggttagta tgggataaag tgtctaaacg taaaaaaatt atgtttaatt
     1681 aaacataatt tttttaatat ctttgagtaa catccatgaa tacaaatgtt tcaggtgtat
     1741 gctttgattc tgtatattca aagagctttc catcatcagt atatacacta tttttaataa
     1801 caccaacaca attataatca tttagaggta gcgtttttaa ctcaagctct gtagcttttt
     1861 caattttaaa tatctttctg gatgaaacaa tttttgtacc tatacactct tctatatatt
     1921 tataaataga gccttgtgct atagaaacat caaggtcttt aactatacta catagaaaat
     1981 agttaatgtc gagaatttta ggtatgttat caatatatct tagtctgtga aggtaatata
     2041 cttcactacc aactttaaaa cctgtctttt tagataactt attatcaatt aagattttgc
     2101 tgaagtggag aacactagta gcagtattaa cctttttatc ttctacaatc gattctacac
     2161 ctttgaaatt gtgcatattt agaaaaaaat cattagaaca actattttct aacactacaa
     2221 cacccttgcc ttttatactt tgaacatatc cttctgtgtt taattgagaa attgctctac
     2281 gtacagtgtt tctgctacat gaaaaccttt caataagttg aagttcagta ggcagcatca
     2341 tattacttgt atatttacca tcaagtattt cctctttcaa tacttcatat atctctgaaa
     2401 atttattttt agccatatac ccctatccct ccttaaaggt ctttagtaat attattgaac
     2461 tgatatgaac aaattatatc aaatgtgttt agacatgtat agtgaaaata gaattatata
     2521 aaccataaaa aggataaatt tgaactattt tgtgggttgt ttcaaataca tggttaaaca
     2581 tattttaaaa ataaaaaaaa taaatttaaa aaataggcta gatatagcaa tatacttaaa
     2641 aggatttaga tatgaaacta aatgataaaa ataaaatgaa taaaaaatat tgacaatttg
     2701 tttaaacaaa tataatattt aaaatataaa atgtgaataa atcgttttca caaaaaatta
     2761 aaccgaaata gaaaggggat tggatatgaa gaggttctta aacaaaatga tgcaacaact
     2821 gtcactttta ggaaagacta tgctggtgcc aatagctgta atgccagcag caggtatatt
     2881 aggattatta tttggtccaa atatgcttaa tgtgccagct atatctaata tatctaatat
     2941 agtattttca aatgtggact acttattttt attaggagca gttagtgctt attcaaagtc
     3001 taaagacaag acaagtgtaa tggttgcagc cgtagttgca tatatgatat ttaagtcaaa
     3061 tttagagtta ttgaatgata cattaaatgc aggtgtattt ggtgggataa ttgttggagc
     3121 ttgtatagca tggacttaca ataaaacata taaagttaaa gttccagcat ttttaagctt
     3181 ctttgcagga gaaaaatgtg taattacact atcaccagtg gttgctttaa ttttatcata
     3241 tatatttagt ttattatgga tatatccaca agctgcaatg aatcaatttg gtttttggtt
     3301 gggaggagct ggtgcaatag gaatattctt gtttttattc tgtaaccgtg cactaatacc
     3361 aactgggtta catcaagttt taaatgctta catattattt gaaatgggtg aatatacaac
     3421 atcaacagga gaagttataa gaggagaaat tcctagattt atggcaggag acccaaatgc
     3481 aggattcttc tggacaggat tttacataat tatgatgttt tcaatacctg ctatttctta
     3541 tgctatatat aaaacagcac aagataaaaa gaagcaagaa gtaaaaggtg caatgaccgc
     3601 tggagcattg acttctttta tggcaacagt aactgaacca atagaatttt cgtttttatt
     3661 tgcatctcca aagttatata taattcattc tttttattct ggtctaggtg gagttgtact
     3721 atatttactg ggtgctcgct tgggttcatt taatggagca agtataattg actatgtctt
     3781 aagttttagt tatggagata aagcgtggtt agttatacct gtaggagttg tattctttgt
     3841 attatattat gtaacattta aatatataat aataaaagat aatgtacaaa caccaggaca
     3901 tgaagttgag atagagatag gtgataaagt tagtgaaaaa gaaaagaatt taaaactttc
     3961 tcatggtaat ttttcatata tggctaagca gataattaaa aattgtggtg gttatgaaaa
     4021 tatagtgact cttaataatt gtatgacgag gttacgttta gaagtgaagg atgccactat
     4081 tttgaatgat gataatatta aaaaaacagg agccaaaggg gtaattaaat tgagtaatac
     4141 atcagttcaa atcatcatag gaacggatgt tgttaaagta aaggatgaga tggaaatgca
     4201 acttgatgag ttgagaaagc aggcagatgc ataaaatgaa gaaattagta gttagggcag
     4261 atgatatagg atattcagaa ggtgttacac tagggataat atcttcttat aagaacggta
     4321 ttgttacaag tgctggaatc atggtgaata tgcctttttc aagacaagct atagaaatgg
     4381 taaaaaatga tgatgtatgt tttggtttac atgttaattt agtaatagga gaaccttgtt
     4441 gtaaagaaca tattaagata aagaatttgt tagatgaagg tggaaaattt gtttctagca
     4501 aaataagaag aacgcaacta ctgacaggta aagaggtttt taattatgaa gaagtatatg
     4561 aagaagtttg tgctcaaatt gaaacttaca agttgtatat gagtaaattg cctgagtata
     4621 ttgatataca tggtatagaa gaagatgtat caatatcagc aatgtgtgat gctgcaaaag
     4681 aatacggtat accaagttgt ccctattatt tagattcatg tattagcatg ccaagtaaat
     4741 atcctcagaa tgatttttac aagttaaata aatcttttgt taaattattt gaagaaaaat
     4801 atataaattt agataaagat ataacattga tggttactca tccaggattt atagattata
     4861 gtcttataaa atcatcttct cttattcaag agagagtata tgaacatgca atgctatgtg
     4921 ataaggaagt taaaatgtgg ttaaaaagaa ataatattga attggttgat tttaaagata
     4981 ttttcaagtc taataattat atataaagaa tagattacct aaagttttaa ttaaaaataa
     5041 gtatttgaaa aaaatgtgta ttttctaaaa aaatattgat agagatgtat caaaataaaa
     5101 acctgaatca tctctatcaa tataggaagt acacattttt tatgtataat aaaaagagta
     5161 gtatgcatta ataaattaaa aatattaaat tataattata ttaacgaaaa ttttagctaa
     5221 tatttttgaa ttttacttat gaataataaa atagatggag gagaaactat ggaattttat
     5281 gaaagagcaa aaaaatatga gtataaattt acagatacag aagacatgat aattgagtat
     5341 atgagaaaaa acaaagaaga aatagtaaaa ttatcaatac aatctttagc agataaattt
     5401 tttactgtcc caaataccat agttaggcta tgtaaaaaaa tggagtatga tggtttctct
     5461 caactaaaaa acaatttaaa agaagagtta aaaaatgagg ataaagagcc aaaaaatggt
     5521 tttgaatcag atataaaaaa gacattagag ctaatagatt atgaaaaaat aaataaagta
     5581 gcagatataa ttcaaaaatc taggagtatt atagtttatg gaataggtca aaacattcct
     5641 ttatgtgaga tattagtaaa agaaattaga ggtgggcatg ataatataac ttactatagc
     5701 caaagacatg aagtacttca tgatatagga aaattaggtg aacaagatat gtttttcgcg
     5761 cttagtttaa gtggagaaac gaaagaagtt atagaagcag caaagacagc agttaataag
     5821 ggttgtaagg tagtagctct aacacatata tgtgaaaatc aattgcaaca aattgcagat
     5881 ataagtttat attgctattc aacaattaaa caagtaaatg ggtacaatat atcagataag
     5941 gttccaatta tgatgattat gagagcaata atagaacaat actggttaag atagagattt
     6001 gtgtataaat acacaaatcc ttatttttta agaagaaatt gtgtgtataa tttcaaaaaa
     6061 acattttcat gatttatttt atgctatatt cataacatta aatgatttaa ggaggtcgta
     6121 attttggaat ttaagaaaat cacaaatgaa aaccttgttt ttttaaaaca aaaaataaaa
     6181 acaaaggaag acgcaataaa gttcttagca agaaagctat atgatgaagg caagttaaca
     6241 tcttatgagg aatatttaga agctgtaatg tacagagaaa ctttatctcc aacaggtttt
     6301 gaaagagggc ttgcaattcc tcatggaaaa tctaagtcag ttaaagaagc tgcatttgca
     6361 gctatgacct tagagacacc aatagaaaat tgggaaagcg tagactcaaa taataaagta
     6421 gagctaatat ttttaatagc agtaccagaa agtgaaggtg gaaatacaca catacaaata
     6481 ctttctgagt tagttactag actgtctaat gaagattata tgaatatgtt gttaaattca
     6541 aaaactaaga aagagttatt taataactta gatataaaag aaaagaaaga acaaataaaa
     6601 gaagagatta ggattcaaaa tgaatcaaaa aaaattgtat tagcaataac agcatgccct
     6661 gcaggtatag cacatacata tatgtcagca gaagcattaa ttaaagctgg caaagaaata
     6721 ggtgtggatg tgtatgtaga aaagcaagga gctaatggta tggtggaccc tcatacacca
     6781 gatataataa aaaaggcaga tgcaataata tatgcaacag atgtagcacc taaaaataca
     6841 gaaagatttg aacatttgcc aaacataaaa acgtcagtag cagctccact aaaaagggca
     6901 aaagaaataa tatacgaagc tttagaagta gcacaaaaac aaggtaaagg tgattatata
     6961 gaaaaatcat ctgatatatc atattgtgaa aaatcatcat ggaaaaaaga tgtaaaatca
     7021 gcaatactta caggagtatc tcatataata ccacttattg tggcaggagg tatgatactt
     7081 gcatttgcag tattagcatc tcaagcattt ggtttacaag aactatacaa tcaagaaggt
     7141 tcttggttat ggcaattaag aaagctttct ggagggatgt taggtacatt gatggttcca
     7201 gtattatcag catatatgtc ttactcacta gctgaaaaac caggtcttgg accaggattt
     7261 gcagcaggtc tagcagctga tttaataggt agtggatttc ttggaggtat gttaggaggt
     7321 ttacttgctg gttatatagt aaaattttta aagaagaata taccagccaa aggcacatta
     7381 gctggattcg taagtttttg ggtatatcca gtagttggtt ctttggtagt aggtttattg
     7441 atgttatttg taataggaaa gcctgtagca gcacttaaca atggtcttat aaattggtta
     7501 aatgggatga ctggaataaa tgcaatacta ctaggtgcaa taataggagc tatggtttca
     7561 tttgacttag gtggaccaat aaataaagca gcctatacat ttgcaatagg agcaatagca
     7621 agtggaaatt ttatacctta tgcaatattt gcatcagtta agatggtctc agcttttgga
     7681 gttacaatag ctactgtttt aggcagagat ttattttcag aatcagaaaa ggaaatagga
     7741 aagcaaactt ggatattagg attggctgga ataactgaag gtgcaatacc atttatgata
     7801 gaggacccaa tccatgttat accatcatta gtagctggtt cagccataac tgggtctata
     7861 gtagcattct tcaatatagg tcttcaagtg cctggagcgg ggatattctc attggcgctt
     7921 ttagagggaa gtatgtcagg atttattata gcatcaatat ggttctttgc ggcagtactt
     7981 ggagctataa tatcagcaat attattaata ataactagga aacaaaaaaa tcaaagaaag
     8041 atacattaaa agatacgtta ttagatattt aaatcaaagg tttaattata gtgataaaat
     8101 aaatattaaa aaattgtgta aactcagtag tatttttatt ggatttttaa ttagacagaa
     8161 aatattatca gataagtttt ggaggataag caatgaaaaa aaatgtacac atagttcctc
     8221 atatgcattg ggatagagaa tggtacttct caactgagga atctagaata ttacttgtaa
     8281 ataatatgga agaaataatg gatatgttag aaaataatca taactatcca tattatgtga
     8341 tggatggtca aactgctata ttagaagatt atttagcagt taagcctgaa tgtaaggaga
     8401 gaataaaaaa attagttcaa gaaggaaggc taataatagg tccttggtat actcaaactg
     8461 atgaaatggt agttggtgga gaatccatac ttagaaattt actgtatgga ataaaagatt
     8521 gtgatgaatt tggagaatat atgaaaatag gatatttacc agattcattt ggtcaaagtg
     8581 ctcaaatgcc gcaaatacta aatgggtttg atataaaata ctcaatgttt tggagaggat
     8641 gttctgaaag aaaaggaact aataaaacag agtttaattg gaagagtgat gatgggtctt
     8701 ctgttttggt tcaaatactt ccattaggtt atgcaatagg aaaatatcta ccaatgaatg
     8761 aagatgagct aagaacaaga atggataaat atttaccggt tcttgataag ggtgcaacta
     8821 ctgaccatat aatacttcca aatggacatg accaaatgcc aattcaaaag aatatatttg
     8881 aagttatata taaattaaaa gaatgttatc cagagagaaa atttttctta agtagatatg
     8941 aaaacatatt taaagagcta gaaaaaaata aggatataga tacaataaaa ggggaatttt
     9001 tagatgggaa atatatgagg gttcatagaa gtatattctc aacaagaatg gatataaaat
     9061 cagcaaatgc tagaatagaa tcaaagataa ccaacatatt ggagccacta gcttctattg
     9121 catattctct aggatttgaa tatcatcatg gattaataga gttaatttgg aaagaaataa
     9181 tgaaaaatca tgctcatgac agcattggat gttgttgttc agataaagtt caccaggaaa
     9241 taatgaatag atttttctta gctgaggaaa aagtagacca attaataaca ttttacaaaa
     9301 gaagaatagt tgatgcaatg agttgtgaaa tggctttaga taagttagta gcatttaact
     9361 taatgcctta caataggaat gaaatagtga acgcccaagt tataactaaa atgaaagcat
     9421 ttgaaatatt tgataaagat aataataagt tggattttga agtggtacat aaagaagtaa
     9481 tagatgcagg attaatagac cgtcaaattg ttcattatgg aaactatgat tcatttatgg
     9541 tatatacaat aaactttaaa gataaaatac ctgcaatggg gtataaggcg tatatgataa
     9601 aagaagttga acatatgtta gaaaaagctt atgaagtatg tgacatggta gataatgact
     9661 tttatactat tcaagttaat gaaaacggga cattaaagat atctgataaa aaattaaata
     9721 aaacttttga aaatgtatta ttgatggaaa atggaggaga tgaaggagat gagtatgatt
     9781 tttcaccact tccaaatgag aaattaatat ttaacgataa tatagtagca aattccacta
     9841 taaagaaaaa tagatttaat aatgagataa agataaatta tagattaaaa gtaccaaaag
     9901 atatacaagc tagaaaaaat aatagagcag attcatttat tgactttgat atagttataa
     9961 atgttccaaa tgataaagct ataataaatg tagtattcaa tataaataac caagcaagag
    10021 attgtagggt tagaacttat ataccaacaa atatagcttc aaagttttct gtgtctgata
    10081 atcagtttgg acatataaag agaagtgtgt atgatgatgc tatggaagtt tggcaacagg
    10141 aagaatggag tgaaagacca gattctatat atccaatgct aacattcgtt ggattgtctg
    10201 acaaagagca tggagtagct gttcttacaa acagtacaag agagtttgaa atagtaggga
    10261 aaaaatttga cacaatagct ataacattat ttagaagtgt aggattccta ggtaaagaag
    10321 aaatggcaag acgcccaggt cgtccatcag gaataaagtt gcctactcca gattctcaaa
    10381 tgataggaga tataaaaata gacttagcaa tagtcactca tgaaaaatct acactagaag
    10441 caaatgttgc aaatatggct aaagagtatt taacaaaaat acaaacttat aataaaatac
    10501 catataatgc tatgaaattg aatcaatcag aaatagaagt agattattca tatagtttac
    10561 ttaaggaaac taataaggac cttgtgttaa gtgttgtaaa aaaggctgaa aaagaagagg
    10621 cattagtagt tagattttac aattctaaag aagaaacaaa atcagctaac gtagtattta
    10681 acaaaaatat taaaacagct aaatggacta acctaaatga aaaagaatta gaatctatat
    10741 caatagataa taactctgta aatgtagagt gcctaatgaa ccaagttaaa actatattgg
    10801 ttaaatagtg ctaaaaatga ttagatagtg ctaaaaagga attatcttaa aatagagata
    10861 atcctcctta tcattgttga aataataaac atatatttaa ttttatacaa aaagagtgtc
    10921 tcatgaacta aaaagttcat tttgagacac tccctttttt ataagataaa agaatattaa
    10981 gggtataagt tgtatacaaa gtgaaatata gagtataaaa taaaagataa attaagctta
    11041 taaaattata tatttaaaat aaagagtaaa taaaaaggag tagataaata tgaaaatatt
    11101 aatttctata gattctttga aaggaagttt atcttcaata gaggcaggaa atgcaataaa
    11161 aaaggggata ttgaaggtta aagaagatgc gcaagtaaag atattgccac ttgctgatgg
    11221 tggtgaaggg acagtagatg ctttagttca agggatgaat ggtaaaaaag agactataga
    11281 agttactgga ccaatagcta aaaaagtgga tgcaacatat ggattattaa aaaatacaag
    11341 tacagcaata atagaaatag ctcaagcatc aggattaaca ttggttccga ctgaacttag
    11401 aaatccttta tacactacaa cttatggtgt tggggaaata ataaaggaag caataaataa
    11461 aggctataga aattttatag ttggaatagg tggaagtgct actaatgatg ctgggatagg
    11521 tatgcttcaa gccttagggt ttgagtttta tgatgaaaat aacaagttag taggattagg
    11581 tggaaaagtt ttaaatgaaa taagacatat aaaaatagaa aatagattaa aggaattaga
    11641 tgaatgtaag tttaaaatag cctgtgatgt aaataatccg ctgtttggga aaaatggagc
    11701 tgcatacata tatgggtctc aaaaaggtgc aacatcagaa ataattgagg agttagataa
    11761 tgggcttaga aatttttcca aagtagttaa aaattactta agtaaagatg tagctaatgt
    11821 agaaggagca ggagcagcag gaggattagg ttttgctttt ttagcatttt taaattcaaa
    11881 attagaatct ggaataaaaa taatattaga agaaattaaa ttagaggaag agttaaaaga
    11941 tgcagatttt gtcataactg gtgaaggtag attggataat caaactgcta tgggcaaggc
    12001 tccaatagga gttgcaaaac tggcaaaaaa atatggggta aaagtaatag gtttggctgg
    12061 agccacaaca gaagatgcag ttaaatgtaa tgaagagggt atagatgctt atttttctat
    12121 agtaaataga gctatgacaa tagaagaagc aatggataaa gcaactgcta gtgagaatat
    12181 gacagctaca acaacacaaa tattcaatct tataactagt atacaacaat caaaaaatat
    12241 gtagtattaa agcaaagtat attaggtaaa aagctgtcac atattaattt agacttttta
    12301 gtgtgacagc ttatctattt tgtgttaata acttaagaaa catatattgg aaataaatta
    12361 atggatttag atattttgaa taagataagc tattctattc ataaaactac tgtagttatc
    12421 tgccttagta agttctttaa cattattcca ctcagataat atttcaataa agctttcaaa
    12481 tatgtaattg aatttttctt tttctttttc atttataggt atcatagcta taatttgaac
    12541 tttttcctca ccccatttaa ctggtttatc gttaacaatt aaacatactc cagtttttaa
    12601 agcatccatt ttcatgctat gaggaattgc tacattgtta ttaaatgcag ttgaagacat
    12661 tttttctctt ataagaacat catctatata attgggtttg acatatttat ttttgtacag
    12721 aatatttccc ataaatttta tgtaatcttt agcactatct aaatatatgt ttttcataaa
    12781 caaagattca ctaaagtaca tttctaaatg attttgtaca gtaagtatat tttttctatc
    12841 tttaatctga ataaatttac tttggatatt atcataatct tttctgttta ggtaaggatt
    12901 tacataaaca aaaggtatag ggcatctatc actaattggt aaagtagtaa taaccatgtc
    12961 taccttattg tcaaaatcaa gattatgagt atctgaaaaa atttgaataa tttctatgga
    13021 ttcattaaat ctcttctcga ggtctcttac tattttgaat tgtaaatcat agtaatttgg
    13081 agtaattaat gcacagagaa ttttatcttc tagtaatttt tgtctatcaa aaaatgaacc
    13141 tacatgaaga gatatatacg atatttcatc ttcatctatt tctatgtttt ctaaaatttg
    13201 aatttgatta caaatataaa cagatatatc atatatcaga gggtaggaat cttttaactt
    13261 ctgaggtatt tgatttctca aaacttgatt atttttagcc ctactgatta aattttttac
    13321 atgaagagta aattttacaa caaattcatc atcagataaa tctataaagt ataagtcata
    13381 tacattttta attatttttt tagttaattt aacaaaatgt tcatctatat aatttggcaa
    13441 tgacttagta tccatttcat gataatttaa gactgtggtt ttgtttgtta actgaaatac
    13501 taagtagtat aattctgcac tagttaaagt taaattgtat tcagactcta aaaaatttga
    13561 tatatctctt gaaatgctta tatcattatt attatctatg tagtttacta cttcgacaga
    13621 ttctatgtag ttattctttt ttattctgtc tatagttatc attaaatgaa gaagtatgtt
    13681 atttatagcg tactcattta taaacaaatt gtattttttt aatatatata ttatattttc
    13741 tttcagtttg gtcaaattat actcttgata tattttttgt acatcaaata agcttaaaaa
    13801 atcacttccc gtttctttaa atattataga gctcattaat ttgcgctttg cagactcatt
    13861 tccaattagt ttcaataaaa atttcgattg ctttatcttt aaattataac ttcctataaa
    13921 tttattagct ttgactatat ccccttcaat agtagctgga ctaacaaaca attcttcact
    13981 taaatcaaat atattaattc cattactatt aactataagt tcttttaaaa ttaaatttaa
    14041 cctgttatca ggtgatatat tttctgaact atcaaccttg gtttgacagt tattattgag
    14101 atttacttta tatccttttt tagaggataa tataaattca ccagtagaat taatttcatt
    14161 tacatatttt ctaatcgaac gtgttgatac attaagataa ttagctaatg tggatgaatc
    14221 tatccaatca tgttgcgttt ttatgaagtt taaaaataac tcttttttag atgttttcat
    14281 tagattccct ctcatagttt taaaataaaa tctgtatgat atgatattta tataattgaa
    14341 tgtacgtatt ataatgtaca ttatactata tatttatgtg tctggttaag agtaaatgat
    14401 ttcctattat taggaaatat atttatgtga aaggaaatta tttaggaagt acaatataaa
    14461 tacaatcaaa tcaaataaaa attcattaag aaataaatta atgaataaaa atactggagg
    14521 tattgttatg aagaatataa aagtgttgtt ggcatgtggc tcaggagcaa gtacaggttt
    14581 tatggcaact agtatgagaa agagtgcaaa gaaaaaaggc atagaagcag atataaaagc
    14641 tagaagtgag tcagagatag ataaatattt accagaaata gattgtttat tattaggacc
    14701 acatttagat tatatgaaag atgaaataca aaaaaaagct gatgaatata atgttccagt
    14761 agcatgtata tctaaaacag catatggaag attagatggt gtaaaggctt tagaagaagc
    14821 attagaatta atattaaaca aataaaatat ctatggggag attgtttatg aataaattta
    14881 tagatttgtt aagtgaaaag tttactcctg ttgtaaataa gataactaag aatatatggg
    14941 tacaatcagt tcaatcaact ataatgaaag ttttgcctat ggtatttgta ggttctttag
    15001 taactatagt ttctgtcttg aagaattaca taagtttctt acctgattta agtccaataa
    15061 atcaatatac atttggactt ttaggactat ttatagcatt tttattacct atggaaataa
    15121 tgaagaataa gaaatttgaa agtatgagtg tagtagctgg tcttgcaggt gctggattaa
    15181 tgttaatgat gattcgtcca gagattacga atgaaggagc tatatttaac ttcaatagat
    15241 ttggaggaga aggaatgctt gtagcactag ttgcaggatt attctccgga ttaataatgt
    15301 ctttatttgg gtctttctca ttttttggtg aagattcagc acttcctgat tttgtaagta
    15361 agtggtttga tagtatgtta cctatagcag taataatgtt tataggttgg ggactaattt
    15421 atggacttaa ctttgatatg tttgaaataa tagttaggat atttaaacca gtatcagata
    15481 tagcacaaac attgcctgga tttgtactat taaacttctt agtagtattt ttatattctt
    15541 taggtataag tggatgggta ttatccccaa taacttatgc aattcagctt agtgcaattg
    15601 cagctaatgc agcagctgta gcatcaggag ggcttgcaac taatatatat acttatgaag
    15661 ttgttcaaac aggttggata actttaggtg ggtggggagc aacattacca ctagtgctaa
    15721 tgatgtgttt atctagagct ggaagaataa aagcaatcgg aagagcctgt atcgttcctt
    15781 ctataatgaa cataaatgaa ccagtaatgt ttggagctcc agtagcatgg aacccatttt
    15841 taatgttacc tatgtggata ggaacagtag taatatcaat aactacttac ttatcaatga
    15901 gtatgggatt tgtgacaatt ccgagtaagg tgtttaattt atggtatctt ccttttggta
    15961 tatctacttt tatggtaaat caagatgtta gagggcttat tgttttagca ataaatatag
    16021 tattgctatt catgatatgg tatccatttt ttaaagctta tgaaaaagaa gaactaaaat
    16081 tagagagtca agaagcttaa atttagatgt tgaaaggaaa ttataaatat gacaactgaa
    16141 gaacatgtaa tagatgaaga attagtagaa gtagcaatgc aaataatact tcgtgcaggt
    16201 gaggctagaa cagaaattaa acatgctctt aatgatttag aaaggtttga ttataaaaat
    16261 gctgatttaa aactagctaa agcaaaagag tttatgacag aagcacatag agctcaaact
    16321 aacattatac aaggggaagc tagtggagaa aaaagagcac attcactttt atttgcacat
    16381 gctcaagata ctcttatgac aatattcagc gagttaaata taactatgtc tttagtagga
    16441 atagtaaagt ctatagaata taaggtaaat aatagtaaat gatttaaata aataaaataa
    16501 ggagtgatta aattgagtaa gtttcctaaa gattttttgt ggggtggagc tgtagcagct
    16561 aatcaattag aaggtgcttg gaatgaaggt ggcaaagggt ggtgcttagc tgatgtacat
    16621 atgtatgata aagatagaga tatatctaaa cattacgagt ctgatatgac tgtagataaa
    16681 ataaagtttg ctatgaaaga taataatggg tattatccta agcgtaatgg aatagacttt
    16741 tatcatacat acaaagaaga tttaaagctt ttgaaagaac tagggttaaa gtgttttaga
    16801 ttttcaataa attgggctag aatatttcca aatggagatg atttagagcc taatgaagag
    16861 ggattaaagt tttatgataa tcttataaat gaagtaatag ataatggtat ggaacctatt
    16921 gtaacactac tccattatga gactcctttg aatttagtat taaaatataa aggatggtct
    16981 aatagaaaag tagtggattt ctttgttaga tatgcagaat taatattaaa tagatacaaa
    17041 gataaagtta aatattggat agttataaac caaataaatt taatgtatca tgaatcattt
    17101 aattcagttg cattttgtgt agaccaggta gatgatgtag aagaggccaa atatcaagct
    17161 attcatcatc aattcatggc tacagcttta actaagaagc ttggaaaaga aataaattca
    17221 gatatgatga tagggatgat gatggcagat tgtacatctt atccagaaga ttgcgaccct
    17281 gaaaatgtag tatttgcttt aaaaagaaat agaatacaat atttttttac tgatgtaagt
    17341 tttagagggg agtatccagc ttatattaag agattcttca aagaaaataa cataaatata
    17401 gttatggaag aaggtgatga taaagtgtta aaagagaata ctatggattt tctagctcta
    17461 tcatactatt actctacaac agtatcagca agtaaaaata ccatgaatcc aattgataat
    17521 gtaaaaaatc aatacttaaa agcaaatcct tgggggtggg caattgaccc taaaggatta
    17581 tacaatactt tatctcaata ttatgataga tatcaaaagc caataatgat agctgaaaat
    17641 ggatttggaa tgcatgataa atttgaagat ggaaaagttc atgatagtta tagaatagat
    17701 tatttaggaa aacatataga acaaataggt gaagctatag ctgatggaac cgaaatatta
    17761 gcttattgta tgtggtcgcc aatagatata gtaagttgtt catctcaaga aatggaaaaa
    17821 agatatggat ttatatatgt agatatagat aacaatggaa atggaagcaa agaacgttac
    17881 aaaaaagata gtttttattg gtataaaaat gtaataagaa ctaatggaga agaattaaaa
    17941 gaaaaagtag atagttttta aatataaata atccctctta taaatactat gttattaata
    18001 cagttttata agagggatta taattataaa taaggtggtt tattaattat gaatgtcata
    18061 aaaaa
//
LOCUS       DAEPOZ010000057        18039 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.057, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000057 DAEPOZ010000000
VERSION     DAEPOZ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18039
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.057"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..1849)
                     /locus_tag="KR440_001504"
     CDS             complement(<1..1849)
                     /locus_tag="KR440_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBH0698560.1"
                     /translation="MKIPKKIAAMLTVTMIAGSSTAGIASAQTVATNLTGQERYETAV
                     KISQDGWKNADEVVIVNDSSIADALSATPFAKAKNAPILLTSKDKLNDKTKAEIQRLK
                     AKKVYLIGGTSVLSTNIEKEIKDLKISFERISGAERYQTSLELAKKLDAISDVKKIAV
                     VNGEKGLADAVSVGAPAAQNNMPVILADSKNGTAVADKFIKDAGITQSYVVGGESSIS
                     EAVKNKLPNSTRLGGTDRNDTNAKVIKEFYKKTDLKNAYVTKDGMNKQDQLIDALAVG
                     VLGAKNQSPVVLVGKNLSASQKSLVNSKSFDKITKVGGNGNETAFNEMKSLQEVKTVE
                     AKTISELKSAIDKATANDVINFKPTSEVKEAFTIQTDKAITVNLNGTYTKTVTINMPN
                     GDVNNYAKVDDVVIDDVKDGTFVNYGKITNLKVNDKNGAKIENNSKGEIGSLTVASGA
                     SQVKVTNGGKITTVTNNSKGTTIDNKGTISSVKGDNSPTISGNSPSSNSSGGSSSSGG
                     SSHGGGSSSSKVDKVVLKNTITAANKLYNEAIEGTNVGEYKVGSKAIYKTAIDKAQAI
                     LDKSGVTQKEVNDAVTALNTATDTFKAGKVVAVDKTALQDAVTAATALHA"
     gene            complement(2274..3668)
                     /locus_tag="KR440_001505"
     CDS             complement(2274..3668)
                     /locus_tag="KR440_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0698561.1"
                     /translation="MTKLNKKLSISISIVVVVVYIISIIINSMFIHRYYLHEKRNILD
                     NVENEIKDKDIDKLKSDITLIEEKNNIDIVYIELDKNHKDKGNIDKINQDLLNAFWKK
                     GLSLNKFWIEENSLNNIDKKSINKIYNQGKTKYSLLVKFIKKDNYLFAISIPIEHSEE
                     TIGIVNRFNIIMGIFSVVIITILTFILSNRVIKPMERLKFLSKDISELNFRTEDIKTN
                     DEIEELAYSINIMSEKLEKAHNELNKRNENLKTFISDASHEMKTPIALIKAYAIGMKD
                     GLDDGTYTDTIIEQAENMTNTINSLLYWAKYEKKERSLCLVDLKERLYKSLKNYELLI
                     ERGSISVKCSVDDKELIISSDEDSIDMVFNNLISNAIKYTNNNEIEINLFEENERIIL
                     SIKNGIDYNMGDDIENMWKPFYVLEKSRSKELSGTGLGLTIVRTILEENNFRYRVEIC
                     DGIIEFYIIFIKVI"
     gene            complement(3661..4308)
                     /locus_tag="KR440_001506"
     CDS             complement(3661..4308)
                     /locus_tag="KR440_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0698562.1"
                     /translation="MNILIADDEISMLKILCAYFKKENFNVFTAKNGEEALDIFYENK
                     IDLAILDWMMPIIDGIEVCKEIKENSNTRVLMLTAKSQSEDEIEALNIGADEYIKKPF
                     DPRVLIIRAKKLINYKNTINIKDLKIDFGSSKVFKGDKELLLTKKELELIRCLINNKG
                     MVLSREKLLDLVWGLDYEGDFRTVDTHIRRLRVKIGEDIIKTYRGLGYSLEDFND"
     gene            complement(4759..5598)
                     /locus_tag="KR440_001507"
     CDS             complement(4759..5598)
                     /locus_tag="KR440_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBH0698563.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
     gene            complement(5622..6380)
                     /locus_tag="KR440_001508"
     CDS             complement(5622..6380)
                     /locus_tag="KR440_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0698564.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNENVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            complement(6427..6900)
                     /locus_tag="KR440_001509"
     CDS             complement(6427..6900)
                     /locus_tag="KR440_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0698565.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSHNGDEHLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            complement(6929..7345)
                     /locus_tag="KR440_001510"
     CDS             complement(6929..7345)
                     /locus_tag="KR440_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0698566.1"
                     /translation="MIHMTGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRRLEDRIEKYKDEGVLVLLDFYGGTPFNTSAIIINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            complement(7392..8444)
                     /locus_tag="KR440_001511"
     CDS             complement(7392..8444)
                     /locus_tag="KR440_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBH0698567.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGTIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPMTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            8840..9520
                     /locus_tag="KR440_001512"
     CDS             8840..9520
                     /locus_tag="KR440_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBH0698568.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            complement(9653..9973)
                     /gene="sugE"
                     /locus_tag="KR440_001513"
     CDS             complement(9653..9973)
                     /gene="sugE"
                     /locus_tag="KR440_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBH0698569.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            10355..11182
                     /locus_tag="KR440_001514"
     CDS             10355..11182
                     /locus_tag="KR440_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBH0698570.1"
                     /translation="MNFYKIAVCQMMTTENKIENINHAVDMVTEAAINGAKIIVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEYGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGVEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
     gene            12003..12677
                     /locus_tag="KR440_001515"
     CDS             12003..12677
                     /locus_tag="KR440_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0698571.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKDSLKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIYNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            12665..13825
                     /locus_tag="KR440_001516"
     CDS             12665..13825
                     /locus_tag="KR440_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0698572.1"
                     /translation="MDKISNMSLKKAFFVLTLSSLIIASVLTTFAYILLNNLYNSIQD
                     KYLKTPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNINMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKSQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNPHFGMGLHISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            14042..14569
                     /locus_tag="KR440_001517"
     CDS             14042..14569
                     /locus_tag="KR440_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBH0698573.1"
                     /translation="MKSFANNITFSRIVFALLMLSSTFCSLPFWICYFWCGISDILDG
                     LIARKLRQQSGIGAKLDSIADFIFAVTILVTVGKNIALPMYLWWCIVAIALLRLISYS
                     IGFFKYRTFSSLHTLMNKITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIM
                     IKSKELNRDIKSIFM"
     gene            14855..15523
                     /locus_tag="KR440_001518"
     CDS             14855..15523
                     /locus_tag="KR440_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0698574.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLKINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKDYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            15526..17994
                     /locus_tag="KR440_001519"
     CDS             15526..17994
                     /locus_tag="KR440_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0698575.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITIGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGHLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGTSENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYMFPLVLSTNVSLI
                     FSFISALPSAIYASRVSPKIAMAGSTTKIKRKIKREKAIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSTLDKIDKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRHTNLKPKDSITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYNSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNISLY
                     KMFLWEGVYYGIFAAIFGSIAGYASAIIINMATIEKLDFTNIPITSILQATIISVLAC
                     IIATLIPLRKVKKMNIIDCIDINL"
BASE COUNT         6205 a         2890 c         2201 g         6743 t
ORIGIN      
        1 tagcatgtaa tgctgttgct gctgttactg catcttgtaa tgctgtttta tctactgcta
       61 ctacttttcc tgctttaaat gtgtctgttg ctgtgtttaa tgctgttact gcatcattta
      121 cttctttttg tgttacacca gatttgtcta atattgcctg tgctttgtct attgctgttt
      181 tatatattgc ttttgaccct actttatatt cacctacatt tgttccctct attgcttcat
      241 tatacaattt atttgctgct gttattgtat ttttcaaaac tactttatct acttttgatg
      301 aagaacttcc tcctccatgt gaagaacctc cacttgatga acttcctcct gaagagttag
      361 aacttggact atttccactt attgttggag aattatctcc cttaacacta cttattgtac
      421 ctttattatc tatagtagtt cctttagaat tatttgtaac tgtagttatc ttaccaccat
      481 tagtcacttt tacttgactt gctccagatg ctactgttaa actaccaatc tcacctttag
      541 aattattttc tatcttggct ccatttttat cattaacctt taagttagtt atttttccgt
      601 agttaacaaa agttccatct tttacatcat ctataactac atcatccact tttgcatagt
      661 tatttacatc accattaggc atgttgatag tcacagtctt tgtatatgta ccatttaagt
      721 ttactgttat cgccttatca gtttgtattg taaatgcttc tttaacctca ctagttggct
      781 taaagtttat aacatcatta gcagtagctt tatctatagc tgattttaat tcagaaattg
      841 ttttagcttc aactgttttt acttcttgta atgacttcat ttcattaaat gctgtctcat
      901 taccattccc accaacttta gttatcttat caaaactctt agagttaact agagattttt
      961 gtgaagctga taagttcttt ccaactaaaa ctactggtga ttgattttta gcacctaata
     1021 cacctactgc tagtgcatct ataagttggt cttgtttatt cataccatct ttagttacat
     1081 aagcattttt taaatctgtc tttttataaa attcttttat tacttttgca tttgtatcat
     1141 ttctatctgt tccacctagt cttgtactat ttggtagttt atttttaaca gcttcagaaa
     1201 ttgaactctc tccacctact acataagatt gagttattcc agcatctttt ataaatttgt
     1261 ctgcaactgc tgttccattt ttagaatcag caagtattac tggcatattg ttttgtgcag
     1321 ctggtgctcc tacacttaca gcatctgcaa gtcctttttc accattaact acagctattt
     1381 tcttaacatc acttatagca tctaatttct ttgctaattc taaggatgtt tgatatctct
     1441 cagctccaga gattctctca aaagatattt ttaaatcttt tatttctttt tctatatttg
     1501 tacttaatac agaagttcca cctattaagt atactttttt agcttttaat ctttgtattt
     1561 ctgctttcgt cttatcattt aatttatctt tacttgttaa aagtattgga gcattttttg
     1621 cttttgcaaa tggtgttgca gatagagcat ctgctataga tgagtcgttt acgattacta
     1681 cctcatcagc atttttccat ccatcttgac ttattttaac agcagtttca tatctttctt
     1741 gccctgttaa atttgttgcc acagtttgtg cactagctat gcctgctgtt gaacttccag
     1801 ctatcattgt aactgtaagc attgcagcaa tcttctttgg tattttcatt ttattttctt
     1861 cccccttgtc catttgatat tacttaattt tttaactttg ttcccgttct aattctaaag
     1921 gatacttaat atctaatttt aaagaggttt ctaactataa tgatttcata attaatttgt
     1981 aattattttg ttacattaca gtcattatgt aaggaataga gaaattttct tattgattga
     2041 aaaatttgta aatttatatt ttaaggtaag tttgattttt atgttaatga attgttataa
     2101 aaaacatggt tttgtataaa aaaagtagcc tttagaatta aaagtgctac tctttttaca
     2161 aataaatttt attatttata cttgtaatta tatattaaat aaccaatttt atccatatga
     2221 aaaacaaata attgcgatat ttttttaatt attctttata tttacatttt aattcaaatt
     2281 acttttataa agataatata aaattctata attccatcgc aaatttcaac cctgtatcta
     2341 aaattatttt cttcaagtat agttctaact atggtaagac caagaccagt accagaaagc
     2401 tccttacttc tcgacttctc taaaacataa aaaggcttcc acatattttc tatatcatct
     2461 cccatattgt aatctatacc attttttatg gataatataa ttctctcatt ctcttcaaaa
     2521 agattaatct ctatttcatt attattagta tactttatag cattactaat caaattatta
     2581 aataccatat ctatactatc ttcatccgaa cttattataa gttctttatc atctacactg
     2641 catttaacac ttatactacc tctttctatc aacagctcat agttttttaa acttttatat
     2701 aacctttcct ttaaatctac cagacacaga cttctctcct tcttctcata ttttgcccaa
     2761 tataaaagag aatttattgt attggtcata ttttctgctt gttcaattat tgtatctgta
     2821 taagtcccat catccaaacc atctttcata cctattgcat atgcttttat aagtgctatt
     2881 ggagttttca tttcatgaga tgcatctgat ataaaagttt ttagattctc atttctttta
     2941 ttcaattcat tatgtgcttt ttcaagtttt tcgctcatta tgtttatgct gtaagctagc
     3001 tcttctattt catcgttcgt ttttatatct tcagtcctaa aatttaattc agatatatct
     3061 ttagataaaa actttaacct ttccataggt tttataactc tgtttgataa tataaatgta
     3121 agtatagtta ttataactac tgaaaaaatt cccattatta tattaaatct atttacaatg
     3181 ccaatagttt cttcagaatg ttctataggt atagatatag caaacaaata attatccttc
     3241 ttgataaatt ttacaagcaa actatatttt gttttacctt gattgtatat cttatttata
     3301 cttttcttat caatattatt taaactattt tcttcaatcc aaaacttatt taaacttaaa
     3361 ccttttttcc aaaatgcatt taataaatct tgatttattt tatctatatt acctttatct
     3421 ttatgatttt tatctaattc tatatatacg atatctatat tatttttttc ttcaattaaa
     3481 gtaatgtctg attttaattt atctatgtct ttatctttaa tttcattttc aacattatcc
     3541 aatatatttc ttttttcatg taaataatat ctatgaataa acatactatt tataattata
     3601 gatattatat aaacaactac aactactata gatatactta tggacaattt tttgtttagt
     3661 ttagtcatta aaatcctcca gactataccc taatcctcta taagttttta ttatatcttc
     3721 tcctattttt acccttaatc ttcttatatg agtatctact gttctaaaat ctccctcata
     3781 atccaatccc catactaaat ctaaaagttt ttccctagat aaaaccattc ctttattatt
     3841 gattaaacaa cgaattaatt ctaattcttt cttagtcaat aataattcct tatcaccttt
     3901 aaaaacttta cttgacccaa aatctatttt taaatcttta atattaattg tatttttata
     3961 atttattagt ttcttagctc ttattattaa aactctagga tcaaatggtt tttttatata
     4021 ttcatctgct ccaatgttga gtgcttctat ttcatcttca ctttgacttt tggcagtcaa
     4081 cataagtacc cttgtattac tattttcctt tatctcttta caaacctcta ttccatcaat
     4141 tattggcatc atccagtcta atatagctaa gtctatctta ttttcataaa aaatatctaa
     4201 agcctcttcc ccattttttg cagtaaacac attgaagttt tcttttttaa aatatgcaca
     4261 aagtatctta agcatactaa tttcatcatc agcaattaat atgttcattt attcacatcc
     4321 ctaaaattca ttattatatt tcatattata tccattactt taattatata aaatatatgt
     4381 tacaggaagt ttacaactat ttataataca ttagttatat attataatat ctcgacaatg
     4441 tcttaacaat atctcgatta tatattttaa tatgtaacta atatattata atattttaat
     4501 taaattaaat aaatttatat ttttttgaaa ttacttaata ttttatatgc aaatgaaaaa
     4561 gcacctcatt ctgattaaaa atctttctga gatacttttt aaaatattaa atactatata
     4621 tagcttatat aagtttaaaa tttaatctat tataaaatta ccctaattgc taaacttttt
     4681 aatataaatt ttccaacatc ttacaaatat aatcaataga tatagaatcc taatttacac
     4741 accaacatat atatttattt aaaatagtcc caaaatacct gtaatagctc ccactactat
     4801 cagtataaac attattttta ttggagaaag gccttttttt aaagcaaaga gtactaataa
     4861 agtaagtacc aaaggaatta atcctggaaa tatcttatca agtaaatccg tttgaagact
     4921 aaatttaaaa tcaccactag tataacttat tggtgtagat aaattaataa acctacctac
     4981 aagtcccccc atgactaaag tacctaatat agaagcccct tctataacct tatttactat
     5041 tccaccttga agtatacttt caacagcttt tttcccatac ttatatccat ttgtccacat
     5101 tccaaaagaa attgaagtca ttattatata ttgagctatt acaaataata taggacctgc
     5161 aattgatgct cctccctcag ttaaaccaat acaaacagct aacactatag gagttataac
     5221 tccttgagtt aaagtatcac caattcctgc tagtggaccc atcaagcctg ttttgatagc
     5281 attcatacca tctccactta tttcatctgc tccattagct ttttcttctt ccatagctat
     5341 tgttattcca tgtataacac atccacatat aggttctgtg ttaaaaaatg ctaaatgagt
     5401 ctccacttct tgttttaact cttccttatc tggatataat tttttaagta ctggcaacat
     5461 tgaatgtgag aaagctgttg attgcaatct ttcataattg taattagatt gcgcaaagaa
     5521 aaaccatctt aaccatgatt ttattacatc ttttttgctt aacgttttct tagatacttc
     5581 attttttata tctgacataa tattttcccc ctcacatgta tttaagatgt agcactatct
     5641 tttccgtact taattgaagt aattgtaaga gcaagtatta ctccaataac agatattact
     5701 attatatcta ctttaaaata aacagccatt agtattccta atatgaaaaa gggcatagta
     5761 aacttgtttc caatcaattt taaattcata gcaatcccaa gtgctggcaa catagcccca
     5821 attacattaa atacatgtaa cacattctca ttcattactt ccaacatacc atctatcgca
     5881 tttggaccaa aataaacaac taataatgct ggtataaaac tcattgcaaa taacagtatc
     5941 tgtggtggta ctatatttat aaatgctact ctatcaacat tccctttttg aacctctcta
     6001 tcagcccaat gtgcaaaaaa agaatttact gtcatttttc caatccaaag tacagttgca
     6061 acagttccca atggtacagc tattgccatt gcagtactta catctagctt agatgctata
     6121 gctaaagcag tacctacata tcctgcaaaa gctgggtccc ccatctgagc tccccctgct
     6181 gatataaaac ctagatatat catatttata cctgctccta taattgctcc ttgaacaggg
     6241 tctcctaata taattcccgt tacaaatcca gccaatagag gtctattcca tgtaaaaaat
     6301 ccaacatagg tcaaccacgg actccaagaa agataataaa aaattgctat taatactgct
     6361 tgaattagtg aaatgtgcat attcttcctc cctattttct tttaaagatt tacaagatag
     6421 tcttttttat aataatgttc ctacatcaat tttctttgca tctggtacta tttgtataaa
     6481 gcatttaaca tcttttgata ttagttctct aaatatttct ttttcttcat ctgacacaga
     6541 tatattttta taaaaacttt ttctaccaag ccctgctccc atacctccaa cattcaattc
     6601 ctttaaatct accccactat ttactaaacc taagacagtt ttagggtatt tgaccaatac
     6661 tattaaatgt tcatctccat tatgtgattt taaaacttca gcacctgatt ctacatcatg
     6721 tacttcaacc ttaacagttg atggagctgc catttgtaaa acttgaacta taaatgggtc
     6781 ctttactact aaatcatcca cgattactat cctttttgcc gatgtaatct tacaccaagc
     6841 agtaataact tgcccatgta ttaatctatc atcaattctt gttaatgcta tattactcat
     6901 aattattcct ccagattttt aatttatttt acaactttaa aacagtttta acatctttga
     6961 ttcctgatat cccaacttcc tcacataaat tcttcaagtc ttttaacttc atagtatctc
     7021 tgtttaaaaa tagctctaaa agcataggta aatttacacc tgtcaaacat tctaactcac
     7081 ataccttagc aaatttattt attataatag cagatgtatt aaatggagtc ccgccataaa
     7141 aatcaagcaa tactaaaacc ccttcatcct tatatttctc aattctatct tctaaccttc
     7201 tactaaactc atctatgctg tcaccatggt ttaatcctaa agtttctaca ttttcctgtt
     7261 tgcctactat taattcagca ctttctaaca ttgctttagc catatttcca tgtgtggcta
     7321 taattatccc tgtcatatga atcatacctt tcttaacctt gtttatatgg attattattt
     7381 agtactctaa tctaattatc tatattaatc attatcttac caaagaaaat atcccttttg
     7441 tatacttttt caaaataagc tggtacttct tctaaattta ttctatgtgt tactaatttt
     7501 tcaacattca tatcaccctt ttgcatatga tacaatccag cactccattc ttttcctggg
     7561 aatggaaaag agtttccaaa ccaagttccc ttgacagtta attcacttct tactatcttt
     7621 tcaaattgtt ctctagtcaa agctacatcc ccatatggta cacctgcata caatactgtc
     7681 cctccctttt tagctaacaa caatacttgc ccacatgtca ttggagtccc agccgattca
     7741 ataacaatat ctgcaccatc tccatcagtt aaccttttta tttcttctac aatattcttt
     7801 tcttttgcat ttatacaaat atctgcccct aactctttcg ccaagtctaa cttctcatca
     7861 aatacatcta cagctattat ttttgtagag ccaaatatct ttgcccattg tattgaaaat
     7921 aatcctattg tacctgtacc aagtactaca acagtatctc caactttagc ttctgatcta
     7981 aaaagaccat gccctgctat acatactggt tctagcgcag cggcagtctc ataacttatc
     8041 tcatctggta tttttattaa attcctctct ttcacctttg tatactctgc aaaacaacct
     8101 ccaagttctt tatttcctat tattgctaca ttattacatc ttgaatacaa tcctttttta
     8161 cattcatcac attcaaaaca tggcatagct ggacaaacag caactctatc tcctatttta
     8221 aaactcctta cttctttacc aacctgtgcc acttctccag aaaattcatg ccctaaaatt
     8281 tctccaacca tatgtggacc tgtctttgaa tattttgata tatcagaacc acatattcca
     8341 gctactttta ctttaattat tacatcatct ttttctaata tttttggtac atccacatcc
     8401 tccactcttg tgtctcttat accatagaat ctaactgact tcactattaa ccacccctac
     8461 tttttatttg agaatatatc cctattgttt atatgttaaa tatctaaagc aactttcatg
     8521 ccaatatatg taagcattgc attcatgcat tttataaaat tatcattaaa tgtgtgtcaa
     8581 agtgtgtcaa aagtttctta tattaaaact tatgtgtgtc aatataaacg acactaacat
     8641 tttatattag ttctcttatt ataagtttta taaaatgtac tttttttgtt atttttataa
     8701 aattgttcgg aaatttccct tctatttgtg atataatatt aaatataagg tatcaatata
     8761 aattttcatg cgaatatcag aacttttttg acactaatgc cttattcaat ataataattt
     8821 aactttttgg aggttgttta tgaaaaaagt gtatcaagga aagactaaag atgtttttga
     8881 attagataat ggaaattact tacttaaatt taaagacgat gtaactggta aagatggagt
     8941 atttgaccca ggcgaaaata gtgtaggtct aagtatagat ggaattggta gagctaattt
     9001 agagacatct gttaagttct ttgaaattct aaacaatgct ggaattaaaa ctcattatgt
     9061 aagtgctaat ttagaagaag caactatgga agttctacca gcaaaagttt ttggtaatgg
     9121 attagaagtt atttgtcgtt atagagctgt tggtagtttc ttacgtcgtt atggttcatg
     9181 tgttgaagaa ggtgctaaac tagattgcta tgtagaagca actttaaaag atgatgaccg
     9241 ttgtgaccct cttataacat ctgaaggttt agaagcttta aacattatga ctcaagctca
     9301 atttgattca atgaaaagca tgactcaaaa aatttctaat atagttcgtg atactatagc
     9361 tgaaaaaggt cttgaacttt atgatatcaa atttgaattt ggattctaca atgatgaagt
     9421 aatcttaata gatgaaatag catctggaaa tatgcgtgtt tataaagatg gtgtaatagt
     9481 tgaccctatg gatttaactg ctcttttatt agataaatag tatatacata taattttcaa
     9541 atataataaa cttataaata agataagcta tgaagtaaag actttttaaa tgttgattat
     9601 ttttaaacat tttctcgcct ttaatttcat agcttattta aattaatata ctctaattag
     9661 tagataatag ctttaaccct acaattccca ccacaataaa gcctatacat gcaattcttg
     9721 atatactaat aggctcattt aagatagcca tgcctacaat agctgtaccg acggtaccaa
     9781 ttccagtcca tattgcatat gaagttccaa gtggaagatg ttttagtgct aatgacaaaa
     9841 aataaaaact tacaatcatt ccaacaacag taagtatact tggagttaat ttagaaaatc
     9901 catgtgaatt ttttagttga aaagcccaaa acacttcaaa caaacctgca acaaacaaaa
     9961 ataaccattt cataaactca tccctctttt caaatgtatc tcttattttt ccaataaaat
    10021 aacctgagaa atattataaa atatctccca ggtttttgtc cttccgtgtt cacaacaaaa
    10081 actatgttgt gggttttatc tcggaccagg ccaatcatta ataattgcaa aaccctataa
    10141 aactctatta attattctat tttatctata ataatactat caacatatat aaaaaaagtc
    10201 aatatatggg ttccatctat aataaaaata acttttatat accttttaat attcttattc
    10261 acttctctcc atacccattt aggtcaatct aagaatatct aatttttatt taatatgtta
    10321 taataacata aaaatattga aaggagtaat ttctatgaat ttctataaaa ttgcagtatg
    10381 tcagatgatg accacagaaa ataaaattga aaatatcaat catgctgtag atatggtaac
    10441 agaagctgcc atcaatggag ctaaaattat agttttgcca gaaatgttta actgcccata
    10501 tgaaaataaa tattttccta aatttgctga agaatatcca ggtgaaacaa caacgatatt
    10561 gagtaaatta gctgagaaac atggaattta tctagtttct ggtagtattc ctgagttaga
    10621 atatggaaaa atctataata cttgctatgt atttgataaa aatggagctc taattggaaa
    10681 acatcgtaaa atgcacttat ttgacattga agttacagga aaagttagtt ttaaagaatc
    10741 agatactctt acagctggaa atgatgtcac tgtaatagat accgaatatg gaaaagttgg
    10801 tatagctatt tgctatgata ttcgtttccc tgagttatca cgtttaatgg ctcttaaagg
    10861 tgttgaaatc gttatcttac ctgctgcatt taatatgact actggtcccg cccattggga
    10921 gttatctata agaatgcgtg ctttagataa tcagatattc tatgttggtg ctgcacctgc
    10981 tagaaatatg aatgcttctt atatagcttt tggaaattca agaatttcag acccttgggg
    11041 aagaataatt gctcaagctg atgaaaaaga atgtattatt tatgctgaca ttgatagaga
    11101 tttaattcct gacataagac aacagttacc acttcttaag cacagaagaa ctgatttata
    11161 tgaacttaat actttgaaat aaaacagaac ataaccccat aaatcataaa ttaagtgtta
    11221 aaaaaattta tggggttctc attaatttga tttaaaatta atttaataaa aataatgcta
    11281 aaattaattt aattaaataa tactaataat tactaatgat tatgctaatt gtcagcattt
    11341 actaatttaa tcactattag ttatttcttt tattaaatat ctttactttt tttacttatt
    11401 atgtcttcta gctttacatg tggattatgg tatttctttc tatttttagt cacatctttc
    11461 tattgaattg cttgcttaaa acaccaatgc acacatgcca gataatgttc acaattatgt
    11521 aaaaatattg ctctaccttc ttcaatgttg atattatctc tcctagttaa tattatcttt
    11581 cctaaaattt cttttaatta aaaaacaaca ttagttgata tatcaagtat attttaagca
    11641 actgaaaaaa taataataat tgatatatca actaattaat gtagattaat tcatttttat
    11701 aatttatata ctttttaatt aatctacctt tttaattaaa atccttcctt tctatttatc
    11761 cattttcaac ttaaattata agatttcatc agctgtgttt ttgcattcca ataaatatta
    11821 aatatcaaat atatattcta ttaaaatttc taataatttc atccttgatt tatttaatat
    11881 ataaaaatat actaacttta atgattaagt tttgaaatat aactaagaat ttgagaattt
    11941 attgagattt ttatactaaa atataaatac tatacaaact taatttagaa gggagcattc
    12001 ttatgaacaa taaaatactc gtcgtagatg atgaggaagg tataataaca ctcttaaaag
    12061 attactttga aatgaatggt tatgaggttt acactgctca aagtggaaaa gactcattaa
    12121 aaaaaatatc caaaaatcca gatatcattc tacttgatat aaacatgcct gaaatagatg
    12181 gaattgaagt ttgtaataga attagagatt atgtatcatg ccctattcta tttcttacag
    12241 ccagaattga aaatactgac aagataaatg gttttcgtgc tggtgctgat gattatattg
    12301 taaaaccttt tgatttagac gaacttggag ctagagtatc tgcacattta agacgtgaac
    12361 aaaggaagtc aaatcaaact aaaattaaat tttataatga tttaacaata gattacgaaa
    12421 aaaggttaat ttgtatatat aataatcaga tttcactttc aaaaaaggaa tttgatatag
    12481 tagagcttct ctctatgaat cctggacagg tatttgataa agaaaaaata tatgatagag
    12541 tctggggata caatagcaat ggaaatagtg atgttataat ggaacacatt agaaaaatac
    12601 gaatcaaact atcaaaacat actcttgaaa attatataga aacagtctgg ggagttggtt
    12661 ataaatggat aaaataagta atatgtcatt aaaaaaagct ttctttgttc ttactttatc
    12721 ttcactaatt atagcatcag tacttactac tttcgcatac attttactta ataatctata
    12781 taattctata caagataagt atttaaaaac acctataaat acaggtatta taaccatagt
    12841 tcaaaacaat gtgaaccaat ttagtgatta tgataaaaca ttacttaata taatcaacat
    12901 acttcagctt gtacttccac ttatattttt tataggactt ttattattag ctgatataat
    12961 tttttacaag gtaaaattaa aaacacccat agaaatcctc aataaaggtg caatggaaat
    13021 atccaacaac aatttagatt tttgcttaga atataataat aatgatgaat taggtaatct
    13081 atgtaatgct tttgaaaaaa tgaggtctga actaaataaa aacaacataa atatgtggac
    13141 tatgattaat gatagaaaac aacttaatgc tgctttttct catgatttaa gaaatcccct
    13201 aacagttcta aaaggttact caaactattt aacaaaatac ataccaacag gcaaacttag
    13261 tgatgagaag attttatcaa ccacacaact tatgtctgag catattaaca gaattgaata
    13321 ctatgtagaa aatatgagta atgctcaacg attagaagat ttagttgttt caaaatctat
    13381 gtcgaatata aacaagttta tagaaaactt agatgaaaat atatctatca ttgctaaaca
    13441 agaaggaaaa tcttttacac ttaaaagtca aataaataat ataaatctat tttttgatga
    13501 aaatatcatt cttagagtcg tcgaaaatat aatatcgaat gcttttagat atgctagaaa
    13561 taatgtatcc atacttatat atcttgaaca agaactcctt acctttgtaa ttgaagatga
    13621 tggtatagga tttagtaagg aatctctaaa attagcttta agacctttct atagagataa
    13681 gactttaaat gacagcaatc cacactttgg tatgggtctt catatctcaa aaatattatg
    13741 tgaaaaacat ggtggctcta tttctattga aaataattct actaacagtg ctaaaattac
    13801 tgctaaattc tcaacaagaa attaaaatga ttttgtaagc tttcattgtt cttatataag
    13861 ctttgttagt ttagaattta taaaatgtat ttcaatttac aaccaatata tatactattt
    13921 tgtagaagtt gtaagagttg cttcaattat aatattttgg caaaaggcat ttgaaacaat
    13981 aatagaaata tatatttaaa tatgtagaat agtatgaaac tagaattttg gaggtgatta
    14041 tattaaaagt tttgctaata atattacatt ctctcgtatt gtgtttgcat tgctaatgct
    14101 tagttctaca ttctgttcac tgcctttctg gatatgttat ttttggtgtg gaattagtga
    14161 tatacttgat ggtcttattg cccgaaagtt gagacaacaa agtggaatag gtgcaaagtt
    14221 agatagtatt gctgatttca tttttgcggt tacaattttg gttactgtcg gaaaaaatat
    14281 tgctcttccc atgtatctat ggtggtgtat tgttgcaata gcattattac ggttgattag
    14341 ttacagtatt ggttttttta agtatcgcac attttcatcg cttcatactt tgatgaataa
    14401 aatcactgga atttctatct ttgcttttcc attactctat atttgctttg gtcttgatgt
    14461 tgcaggaatt atgatatgta tcgtggcttt tctttcttct tttgaagaaa tagcaattat
    14521 gataaaatca aaagaattaa atcgtgatat aaaaagtata tttatgtgac aacttagaat
    14581 ttgtgacaaa aataatttat ataataatga ggaagtaaaa cataaaaaat tatccacaag
    14641 tttatgtgta aaatagtttt aaagcaatag agttgtattg taaagccttt agtgcataaa
    14701 ttacatttta aattaaaaat gatactgaaa ctgcttatca gcactgcgaa ttatccgtaa
    14761 atagtgaaga ctttttagat ttaagctaaa agttgggaaa aaattgagtt ttaaaactta
    14821 cactcttctt atatagaaaa ggagagtgtt ttttatgatt ataaaagcaa aacaattatc
    14881 aaaaatatat ggttctaata acaataaagt tatagcactt aataatgtaa atttaaaaat
    14941 aaactcaggt gaatttgttt ctgtaattgg accttctgga agtggaaaaa gcacactttt
    15001 acatatttta agtggattag ataatcctac ctctggacaa gtgttacttg atgataaaga
    15061 tatatataaa cacactgaaa aagaactatc tgcacttaga agaaaatcct ttggattcgt
    15121 ctttcaacaa tttaatcttt tacctgttct tacagcatct gaaaatattt caatgcctgt
    15181 acttctagat aaaaaacaac ctgataaaga ctatttaaat gaaatttcat cattacttgg
    15241 aattgcagat agactcaatc atcttcccca tgaactttca ggcggtcagc aacaaagagt
    15301 tgcaatcgct agagcactta ttgcaaaacc agatataata tttgctgatg agcctacagg
    15361 aaacttggac agtaaaagtg gcagtgaagt tatgaatctt cttataaaaa cttcaaaaca
    15421 gtttggaaag actttagtgg taattactca tgatgataga attgcaaaac ttgctgatag
    15481 aaaaatttca attattgatg gtgttcttat ggaggtgaaa tagaaatgaa gaattacctt
    15541 tcactttcaa taaaagaatt aaaaagccag aaacttatga ctacattcat cattatagca
    15601 atagtactat caactattat gacaactgtg gttggacagt caattggtat cctacaaaat
    15661 ttaagaattg aacaagcccg ttcttttaat ggtgatagac atgtatcctt tcaccaactt
    15721 actaaaaatc aagttgatga cataaagaaa gatgatagag tttatcaagc tggtacttca
    15781 ataactattg gcagtagtaa aataaaggat tctggtatct ctgtacttgt aaaagagtat
    15841 gataaaacag gactttctaa ctacccaaaa ttaatgaaat taaaatctgg acatttgcct
    15901 aaagataaaa acgaaatagc cttagatgaa aacactctaa agcttatggg tataaaacca
    15961 agactaggag tgactatacc tatgaattta gatatttcac ttttaaatga taccatacca
    16021 ccatataatt acacagctaa ctttaaattg agtggcattt tagaggatga ttatacagga
    16081 tatgtaagtg gtatagtaaa tggtattgta ggtaaaggta catcagaaaa tctattacct
    16141 aagagatata ttttatcctc tttggatttt aaaataaaac agcaagaaaa gtttcaagaa
    16201 atagtaaatc aattagctaa aaaaataaat ctttctcaca attcaattca atataattgg
    16261 atttacttaa atgctcttaa gatacagttt gaaaaagatg aaaatagttc taatagtgat
    16321 ggtatatcta tgattatatt ggtatcttta tttgttgcct tattggtttt actggcttct
    16381 ggacttgtaa tctataatat tttaaaaatc tctgttacaa agaaaattaa agagtatgga
    16441 tgtttaagag caataggagc tgaaccaaac cagatatata agatagttat tttacaaatc
    16501 ttaattttat gtactgttgc catacctatt ggagctgtaa ttggaattat ctcttctaaa
    16561 ggcataactg gaatggtcac taatatctta aatccagata tacttctagc aaatgataat
    16621 aaagaaatta ctgagcttat acataaaaat acaacagcat atatgttccc attagtatta
    16681 agcactaatg tatctttgat tttttcattt atttcagcat taccatcagc aatatatgct
    16741 tcacgtgtct ctcccaaaat tgctatggct ggtagtacta ccaaaatcaa aagaaaaatt
    16801 aaaagagaaa aagcaataaa aaactttgaa agacatctag catggctaaa cttaaaaaga
    16861 aataaaggaa gaactataat aactatacta tcattattta tgagtataac agtattcgtt
    16921 gcattgagtg agttctctaa tgtattagat gtatctaggt cagtaagcaa tttaaaagaa
    16981 ggagattttt ccttaacaaa tgaaatatct ggttttgata aatcaacact agataaaata
    17041 gataaaatga aaaacgtcaa tcgcacttcc tttataaagt actctgaata caaacagggt
    17101 gaaatagata cagatataaa ttttgaaaat agtggtgaga tgttaaaaat cattggaatt
    17161 gacgagcaga ctttaaaaga cttaatgcct tctattacag attctatact agaagacttt
    17221 aaaaatggtt ctatctgttt tataaaaaat ccgattgcta tttctacacc aggtgtaaaa
    17281 actagacata caaatttaaa acctaaagat agtatcacaa tcaacaaaaa acaactagat
    17341 atatatagta cagtagataa aatgttcttt ttacaaggta atggctggat aaatggtgtt
    17401 gatgttattg tatataattc tgtttataac accttgacaa ataaaaacaa gttcaaccag
    17461 ataaatattt atgctaaaga taaatcaaaa ttagaacaaa taagattgtc tattgaacag
    17521 atttgtgaaa ataatcctgg ctctcattgg atatcatata ttgaatcaga taagcaactt
    17581 aaagagagtt ttaaacaaat agaatttcta gcatgggcag ttatattatt tgtgggattg
    17641 attggtactt taaacatcat taacacaact catacaaata taaatactag gactaatgag
    17701 attggagtta aaagagctat tggtatgagt aatattagct tatataaaat gtttttatgg
    17761 gaaggtgttt attatggaat ttttgctgct atatttggtt caattgctgg gtatgccagt
    17821 gctattatta taaacatggc aactatagaa aagcttgatt tcactaatat accaattact
    17881 tcaattttac aagctactat tatttctgta cttgcatgta ttatagctac tcttattccg
    17941 ttaagaaaag ttaaaaagat gaatataatt gactgtatag atatcaattt ataactattt
    18001 tacagataat aatataaatt aaattatcaa tgactgtct
//
LOCUS       DAEPOZ010000058        17412 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.058, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000058 DAEPOZ010000000
VERSION     DAEPOZ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17412
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.058"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..767
                     /locus_tag="KR440_001520"
     CDS             <1..767
                     /locus_tag="KR440_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBH0698576.1"
                     /translation="VIPLFKNLLINGNVLRPNYKKDMIPVSMGIVFLPMIVINGIILA
                     FFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVSGLKGHFKSLFKGSLTTGGFK
                     ALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNLLDLRPGRAIKAYLFIMVIIF
                     LTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSNVLGISIGMLISFGYPFNIRL
                     GWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            772..1839
                     /locus_tag="KR440_001521"
     CDS             772..1839
                     /locus_tag="KR440_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HBH0698577.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGKIQRAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            1841..2377
                     /locus_tag="KR440_001522"
     CDS             1841..2377
                     /locus_tag="KR440_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBH0698578.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            2445..3050
                     /locus_tag="KR440_001523"
     CDS             2445..3050
                     /locus_tag="KR440_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HBH0698579.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKNR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTVSIGIIKFLAK"
     gene            3062..3976
                     /locus_tag="KR440_001524"
     CDS             3062..3976
                     /locus_tag="KR440_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBH0698580.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAVEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            4018..5190
                     /locus_tag="KR440_001525"
     CDS             4018..5190
                     /locus_tag="KR440_001525"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBH0698581.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIDGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            5237..6049
                     /locus_tag="KR440_001526"
     CDS             5237..6049
                     /locus_tag="KR440_001526"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBH0698582.1"
                     /translation="MFEKIAQSSKFINSKSNIKPKIGLILGSGLGDLANDIEDPVTIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            6253..7578
                     /locus_tag="KR440_001527"
     CDS             6253..7578
                     /locus_tag="KR440_001527"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBH0698583.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEVDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSIKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
     gene            7791..8870
                     /gene="mltG"
                     /locus_tag="KR440_001528"
     CDS             7791..8870
                     /gene="mltG"
                     /locus_tag="KR440_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBH0698584.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFPYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
     gene            9042..9716
                     /locus_tag="KR440_001529"
     CDS             9042..9716
                     /locus_tag="KR440_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBH0698585.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFASIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            9703..10950
                     /locus_tag="KR440_001530"
     CDS             9703..10950
                     /locus_tag="KR440_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBH0698586.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRAPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            11022..12686
                     /locus_tag="KR440_001531"
     CDS             11022..12686
                     /locus_tag="KR440_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBH0698587.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDKSMKKELEKNGIMVEEKTMRYAKDGLLS
                     HTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKTVD
                     NKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQNDIS
                     KSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNTNE
                     ILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGLDE
                     EKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNMNT
                     IKTYKSSKKEELISPYVCTQVKEYMKSVSRIGTAKDLKDIEGGCGVKTGTAQSSLNKK
                     AIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(12783..13067)
                     /locus_tag="KR440_001532"
     CDS             complement(12783..13067)
                     /locus_tag="KR440_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH0698588.1"
                     /translation="MCNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTPRER
                     EIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            complement(13090..14607)
                     /locus_tag="KR440_001533"
     CDS             complement(13090..14607)
                     /locus_tag="KR440_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH0698589.1"
                     /translation="MSVAIYLRKSRADEEAEKQGEFETLSRHKSTLLKLAKEQNLDVI
                     EIKEELVSGESIIHRPKMLELLKEVEENKYDAVLVMDLDRLGRGDMKDQGIILETFKE
                     SKTKIITPRKTYDLTDEFDEEYSEFEAFMARKELKLISRRMQRGRIKSVEEGNFIGTS
                     APFGYDAVTTGRKERILVPNKDADVVRTIFDLYINEDMGCSKISKYLNNLGIKTATGA
                     NWYNSAITNIIKNKVYCGYIQWQKKDYKKSKNPNKIKTVKLRPKDEWIEAKGKHEPLI
                     SEITWKKAQNILKKNGHVSYGNQIKNPLAGIVICKNCGRPLVYRPYADHDYIICYHPG
                     CNKSSRFEFIEAAILKSLEDTVKKYQLKACDLDLDKNNKDSNIEFQKRVLKGLETELK
                     ELGKQKNKLYDLLERGIYDEDTFIERSNNISSRTEEIKDSIKTVKNRLSTVKKDNSKI
                     IEDIKTVLSLYHDSDSLGKNKLLKSVIDKAIYYKSKEQKLDSFELMVHLKLHEDQ"
     gene            complement(14732..15553)
                     /locus_tag="KR440_001534"
     CDS             complement(14732..15553)
                     /locus_tag="KR440_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBH0698590.1"
                     /translation="MIISKKITAILVSFLIMISMVGCAPKESATEVAKKYLDSIEIKD
                     YEVAYNLLSKDSKESVSLEKFEEYQDTLFKLQTLKDYKLGEENKVEKYKYSNKEYTNL
                     VKIKETDTIKYITENKEDTANFDRYFIIEDNEYKLLWHKDFKRALSSNYVKIARSETK
                     KDEDKDYNKAAIDIKKAIEIDDKNPLAYYTQSYIYIELQRYDEALKSIDKSLEYLDKN
                     NEAYKIDASDAYNLRGVIFMSKSEFNKARESYNKALELNPDNEYAKTNLNDIKGL"
     gene            15744..17171
                     /locus_tag="KR440_001535"
     CDS             15744..17171
                     /locus_tag="KR440_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp26"
                     /protein_id="HBH0698591.1"
                     /translation="MKIKKLLTIGLSLSIFIASCPISANALDKIESIKGADKYETAGI
                     IADKQNYTTAILINADSTMADGLSASGLAGAINAPILLTKKNNIPNATLKRLEKAKKV
                     YIIGGENSINKYTETVLKGKGIEIKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAF
                     KGEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMNDKLVKDTNSTR
                     LGGTDRYDTNKKIINKFYSGVKEFYIASGTDLVYALVGSTIAKNNAIVLVGNDSNKSV
                     LKSTIKLTAIGNLSDSILQQCLNVTKNIGDSNTGREYTVEDAANAVKKLLGVKKFYYE
                     PNYSEDEDLIFLLDKNFDFSGKEYYVFAYIKQGAEGDVRYCVAKNDMSKVYTYDVNGN
                     MKVCNPDDYKNKGSITEEQAKQIALKECAKRHKVSINNLVVDMIDFRDDIYSGNVYAV
                     KINSKNSNGTFGWFFIDIETGKIYN"
BASE COUNT         6753 a         1936 c         2980 g         5743 t
ORIGIN      
        1 ctgtaattcc actatttaaa aatttattga taaatggaaa tgtacttaga ccaaactata
       61 aaaaagatat gataccagta agtatgggaa ttgtattttt gcctatgatt gtaatcaatg
      121 gaataatact tgctttcttt actacagaat ttaaggattt agcgtatata tttatgttta
      181 tgtttggtat gatatccatg ttttttgcag ggatactaga tgatgtcatt ggaaatagag
      241 atgtaagtgg tttaaagggg cattttaaaa gtttatttaa aggaagtctt acaacaggag
      301 gttttaaagc tttatttggt ggatttgtag ggcttatagt ttctgttgct atatcaaaag
      361 acataattga tataatagta aatacactaa taatagctct atcaactaat ttaatgaatc
      421 ttttggattt aagaccagga agggcaataa aagcatattt attcataatg gtaattatat
      481 ttttaacatt gacaggattt gttcaagttc ttccattact tatagtacca aatgtacttg
      541 catactttaa ttatgattta aaagcaaggg ctatgatggg agatactggt tcaaacgtcc
      601 ttggaatatc tattggaatg ctcatttctt ttgggtatcc atttaacatt agattaggat
      661 ggttaatatt tgtagttatt atgcatatat tcactgagaa atactcatta actaagataa
      721 tagaaaaaaa taaactatta aattttatag ataaattggg aaggtagtat tatgataagc
      781 aaaagagtgg ggattgttga atcaatagta agtcaaactg aaactctaga tgatataaga
      841 gtaaatataa atggaaaaat acaaagggca tataattatc ccaaaatatc tggaactata
      901 aatataggtg atgaagtggt tttaaataca acagcagtag aactgagctt aggtacaggt
      961 ggataccatt ttgtaataac taatttaaat aatatagaat ctactttaac tgaaggtggg
     1021 catataatga aacttagata tacacctctt caaataaaag tggattctgt tgaggaacaa
     1081 gaaagtattt atcatgataa atttgcgaat tttaaaggat tagaaggtct ttctgttgta
     1141 gtagggactc ttcatagtat gttaactcca tttgcagctt catttaaaag aaataatcca
     1201 aataaaaaat tagtatatat aatgactgat ggagcatctc ttccaattta tttaagtaaa
     1261 aatgtggaca ctttaaaaga aaaaaaatta atagatagta cgataactat aggaaatgcg
     1321 tttggtggag attatgaatg tataaatata tatactgcat taattacagc aaaagaaata
     1381 ctaaaagcgg atgcagtatt tgtcagtatg ggacctggaa ttgctgggac aggaacaaaa
     1441 tatggattta cagggattga acaaggctct attcttgatg ccgtaaaaaa acttgaagga
     1501 agagcaattg caattccaag aattagtttt gctgacaaac gagaaagaca tcaaggtatt
     1561 tcacatcata gtatgactgt atttaaagaa attgtaaatg taaatgtaga cataccaata
     1621 actatatatg atgatgaaaa attgaacttt ataaaagagc aaatcaattt aaatggttta
     1681 gacaaaaagc ataatataat ttatatagat aataataaaa cgaaagagga tttagactat
     1741 tttaatctaa aagtaaagag tatgggaaga aattatgaac aagatgaggc atttttcaaa
     1801 gctgcaagca cagcagcata ttacttaatg gaggtttaag atggtattag aagaaaaaac
     1861 tattagtagt gatagggttt atacaggtaa agtaattact ttaaaggttg atactgttga
     1921 aattccaggg caaggatatc aaaaaaggga gttagtagaa gttggtggag cagtaggaat
     1981 agttgcaatc acagatgata ataaagttgt actagttaag caatttagaa aacctataga
     2041 aaagccaatc tttgaaattc cagctggtaa actagaaaaa aatgaaagtc caaaagaatg
     2101 tgctgaaaga gaattaaaag aagaaactgg gtatagtgca aaaaatatta agctaataca
     2161 taaatttttc acttcagcag gtttttctaa tgagataatg tttgtatatt tagcaacagg
     2221 tcttactcca ggagagaata atttagatgc agatgagttt ttagatgtct atgaaattga
     2281 gcttgaagaa gcatataaca tggtattaaa aaatgacgtt gaagatgcaa aaacatctat
     2341 tggattatta ttagtaaaag atatgtttaa aaattaatat ttataaaatg aataaccctt
     2401 tgtcctaaag catattattt tataaaggac aagggggaat taaattgaga agaacatata
     2461 gaaaaaatga ttttagagaa ttaaatagac aaataattat tataggattg ttgtttatga
     2521 tgtctattgt tgttgggtct tacataaata aaatattgcc aggttctagt aacaatatat
     2581 taaacaatat aaatcctgct gtagagtatt ataatctaaa tataagcata aaagatacag
     2641 tgatacaaaa tttaaaatca gatgcaatct ttatgggctc aatagctcta ttaagtctat
     2701 ttgtagtaac aattccagca gtgttggtag cttttgtatt aaaagggatg tcaattggtt
     2761 atacaataaa tagttgtata ttggcattaa aatttaaaag tataaaaatg attttaataa
     2821 tattatttaa aaatttgatt attattccat gtgcaatcat attagcatta attagtttaa
     2881 gttattttaa agaaatggtt tatgaattta aaaagaaaaa tagaaaaaat atgcaatttt
     2941 taattaagag gtatatacta aatattataa ttataatagc actatcattg gggttgcaat
     3001 taatattaaa tactgtaagt attggtatta ttaagttcct agctaaataa aggagtttat
     3061 aatggatatt atagagggat atatagatta cataaaagat aaaaaaaaat tatcagagaa
     3121 cacagtggca tcatacttta tggatataaa aaaatatatg gaatatataa atcaaaaaag
     3181 cattaaacta gtagatatag tagaaaatga tattataagt tacttaataa agttggaaaa
     3241 agataatgta tcaatagcta caatagcgag gatgatatcc tctataaaat cttttcatga
     3301 ttatttattt ttaaatcata tatgtacaaa taatcctgca aaggatataa aaaaacctaa
     3361 aataaaaaaa gaaaatataa atatacttac agaagaagaa attgaaagac tattaaattt
     3421 tccaaagtta acaacaccta aattgattag ggacaaggct atttttgaag tattatatgg
     3481 tactggtata aaagtatctg aattagtaga gatgaatata gaggatattg acctagatat
     3541 tgattatata tattgtaatt catgttgtaa aaatcaaaga gtgataccac tttgtgatat
     3601 tacaaaactt tacttagaaa agtatctaaa agaagcaaga cctaaaatgg ctgtagaagg
     3661 tgaaaaatca ttattcgtaa gttcattagg tcaaaggttt acacgacaag gtctttggaa
     3721 agtcataaaa aaatattcta atctagccaa tatagataaa aatataaatc ctacaatgct
     3781 tagacattca tttgctattc atttgttaaa tgaaggtgct aacattgctg ttgtaagtaa
     3841 aattttagga aatgttaatt tatctagttt gcaagtatat ttaaatcata tagataaaaa
     3901 cgtgagaaga gaaataaagg aaaaacatcc tagaaatgat gttgatgtag aattacaaaa
     3961 agcagaagct aaatagacta tatagcaaat tttataattt aaaaggagta tatttatatg
     4021 agcagagtaa tttggattgt aattgatagt gttgggatag gagcattacc tgatgctgaa
     4081 aaatttggtg atagtaagga tgtaagtacc cttggaaata tatttaagga atatccagat
     4141 attcagattc ctaatatgag aaatttaggt ataggaaaca tagatggtat agattttttt
     4201 gaaagtataa aagaaccaat aggttgtttt ggaaagtgta aggaaatgtc tcagggaaaa
     4261 gatactacta caggtcattg ggaaatgact ggtataatag ttgacaaacc atttaagaca
     4321 tttgaacatg gattctctaa ggagataata gaagagtttg agaaaaaaac tggaagaaaa
     4381 gtagtaggaa ataaaccagc ttcaggaact gtaataatag atgagtatgg agagcatcaa
     4441 ataaaaactg gagatgtaat agtatataca tcagctgata gtgtatttca gattgctgct
     4501 aatgaagaag taattcccct agaagaactc tataatatgt gcaaaatcgc tagggaaata
     4561 atgatggggg ataatgcagt agctagagta attgctagac cattcatagg taagaaaaaa
     4621 ggtgagtttg tgaggacttc aaatagaaga gattattcac tagacccatt tgaaccaaca
     4681 gttcttgata atataaaaga atcaggactt gatgttttag cagttggaaa aatagaagat
     4741 atttttaatg gaaaaggaat tacagatgct atccacacta aaagcaatat ggatggagtg
     4801 gatgaaactt taaattatat gaaacaagat aataaaggtc taatatattc aaatctcgtt
     4861 gattttgatt ctaaatatgg tcatagaaga gacccagaag gatataagaa agctcttgaa
     4921 gagtttgata gtagacttcc tgaaataatg gctaatatga gagaagatga tattttaata
     4981 atcaatgcag accatggaaa tgacccaaca tataaaggta cagaccatac aagagaatat
     5041 atacctgtta tgatttatgg caataagatt aaaaaaggtt ttaatttagg agtaaaagat
     5101 acttttgctg atattggagc aactgttgca gatatactaa atgtaaagtt gcctaaacat
     5161 gggtcaagct ttaaagaaga tttattttaa actataaaat gataaaaact tagcttaata
     5221 ggatggagag attggaatgt ttgaaaagat agcacaaagt agtaaattta taaattctaa
     5281 aagtaatata aaacctaaga ttggtttgat attaggttct ggactaggtg atttagcaaa
     5341 tgatatagaa gaccctgtca caattaaata tagtgaaatt ccaaacttcc ctgtatcaac
     5401 agtagcagga catgctggac aacttgtaat tggaaaactt gaaggaaaag aagttattgc
     5461 tatgcaaggt agatttcatt actatgaagg atattctcag aaagaagcta cttttccagt
     5521 aagggttatg aaagaattag gagtcgagat tcttatagta actaacgctg ctggaggcgt
     5581 aaataaagaa tttaaagcag gagatttgat gcttataaga gaccatataa actttagtgg
     5641 aagtaatcca ttagtaggaa aaaatgatga cagatttggt gctagatttc cagatatgtc
     5701 agatgcatat tcttctaaat atgtagatgt agttaaaaac tgcgctaaag aatgtaatat
     5761 agatgtcaaa gaaggcgttt atatgttttt tagtggtcca aactatgaaa caccagcaga
     5821 agtgagaatg gcacaaattt tgggagctga tgctgtaggt atgtctactg ttccagaagt
     5881 catagtagct tctcactcca atataggagt tattggtata tcttgtataa caaatatggc
     5941 tgctggcatc ttaaatcaac cactaagtca tgaggaagta atagaaacta ctcaaaaagt
     6001 taaatcagaa tttatgaatt tagttaaaac aacggttaaa aatttataag ttaaagctta
     6061 ttatatattt tttgaggaag tttataataa aatagttaat gtttaatttg aaaagaatta
     6121 ataaaaagct gtcttgatat agaaagttat ttctaataaa ggacagtttt tttataatac
     6181 tacaacattt gaaagtgttt taaagtttaa tatttattta aattattata gattagaatg
     6241 gagatttcaa ttatgagaat gtatgatatt ataaaaaaaa agagagataa cttagagctt
     6301 agtaaaaaag agataaacta ctttatagaa aactattcaa agggagatat acctgactat
     6361 caagcttctg cactattaat gtcgatttat ttaaataaga tgaataaaca ggaaactgta
     6421 catcttacag aagcaatgat gaactctgga gacatgataa acctgtctga ggtagatgga
     6481 ataaaggtag ataagcatag tactggtggt gttggagata agactaccat agctcttata
     6541 cctttggttg cttcatgtgg agcaccagtt gcaaagatgt caggtagagg tcttggtcat
     6601 actggtggta ctattgataa attggagtct ataccaggtt tttcgacaga aatggagata
     6661 agtaaattta ttgattctgt aaataagtct aaaatagcag tttgtggtca aagtgcaaag
     6721 gtagcaattg cagataagaa aatatatgct cttagagatg ttacagcaac tgttgataat
     6781 atatcattga tatcatcaag tgtaatgtct aaaaagttag cttcaggagc tgatgcaatt
     6841 gtgttagatg taaaaactgg tgatggagca tttatgaaga cattagaaga gtcttttgag
     6901 ttagctaaat ctatggttga tataggtact ggtatgaata gagatacaat agggattgta
     6961 acagatatgg atgaaccttt aggttttgca gtgggaaact ctttagaagt tatagaagca
     7021 attgagacat taaaaggaaa tggccctaaa gaccttgttg agctatgtga agtattagga
     7081 gcatatatgt tggttttatc aaaggtagca catgattttg aagatggtgt tgaaaagata
     7141 agagaatcaa taaaatctgg tactgctata caaaaactca aagagtttat agagaatcaa
     7201 ggtggtaata aagatgtagt agatgattat tctttattca aaaaatcaaa gtataaatat
     7261 ccagttaaat caattaaatc aggttatata agtaaaatta aagctgaaga tgttggtgta
     7321 tcagctatga ttcttggagc aggtagagaa actaaagatg atattttaga tttatcagca
     7381 gggataattt tagaaaagaa agtaggagat tatgtaaatg aaggtgaagt tttagcttac
     7441 atgtattata atgatgaaca aaagctttca ttggcaaagg gaagatttat tgattcctac
     7501 tctattgtag attcaaaaat agaaaaaaat aaattaatat atggtattgt aactaaagaa
     7561 gagattaaaa agttttagaa aattttagaa aagtgattat aaaaggccta acatgtcaaa
     7621 aataatgtta ggttttttat tttttgataa actataaatt ataccaaaaa tatatatttt
     7681 aaaacatttt ttacaatata aataagttta ttctacaaga tgattgaaaa ttaaagtatc
     7741 tagttttata atagaaaatg gataaatata cataaggagt agattatatt atgaacttta
     7801 aagaaaatag attgaaaata gcagtattaa taattgttat tttgattatc ttagcaggta
     7861 tatttgtgtt tatacaaata ggtccttatg ataaaaataa caaaaaagat gtaattattg
     7921 acgttccaag tggagcatct gttggaaaaa tatctgacat attatatgaa aataaattaa
     7981 taaagaatga actattattt aaacttttgg taaaggttag caataaagct ccgtctataa
     8041 aatcaggaac atacttactt aatcaatcgt attcaaacaa tgatataata agtcttttag
     8101 tttcaggtaa aatatatcag gatggtataa aagtgactat tccagaggga gctacatcta
     8161 aagaaattat agcaatgtta gtaagtaaaa atttaggaga taaagctact tttgaaaatt
     8221 taataaaaaa acctcaagaa ttttatgata aatttccata tttgaaggaa gatggaataa
     8281 cctctttaga agggttcttg tatccagaaa cttattattt taattcaaag aaacaatcag
     8341 aagaagatat tttaagcgaa atgttaaaag tatttgacag taaatacacg gacaagttta
     8401 aaaagaaaca aaaagaatta aatatgactc ttcaagaagt aatggaaatg gcatcaataa
     8461 ttgagaaaga agctgttctt gacaaagaca gacctataat agcaagtgtg ttttataata
     8521 gacttaaggt aggtatgcct cttcaatcag atgctacaat acaatatata tttgaagaga
     8581 gaaagaaaat tgtaacatat gatgatttaa aaattgattc accatataat agttataaaa
     8641 ataagggatt acctcctaca ccaatatcaa atcctggtat aaaatctatt gaagcagcat
     8701 tatatccaga gaaaacagac tatttatatt ttgtagctaa gattgatggt ggaaataatt
     8761 atagtacaaa ctatcaagac catttaaaat acgttaaaga atataaagaa gcaagggata
     8821 aacaatcaaa agatacaaaa gcaacaaata aagaaaatac aaaaaaataa accatgatta
     8881 taaaaagtct aacagggtaa gaataatgtt gggctttttt attgaaaatt gttattctta
     8941 gatttataat aaagaaagtg taaatatata taacaatatg aatttaaaaa ataataaatt
     9001 aatataaaac ttgaatattt attattttag ggagtgtttt tatgagtaat atagttaatg
     9061 atttggttga agattatata agagtgacat taaaagaaaa agaaggattt ttaaaagatt
     9121 tagaaattta tgcagaggaa aatagtgttc ctataataca taaagaagtt tcagatttat
     9181 taaaagtctt gttaaaggtt caaaaaccaa agagaatttt agaagtagga tgtgcaattg
     9241 gttattcttc tatttttttt gcaagtatta taggaaaaga tgcagatata ataactgttg
     9301 agagaaatga aaaaatgata gaaaaagcaa aagaaaacat aaaattagct ggatttgaca
     9361 ataacataac tatactagaa ggagatgcag aagaaaaact tagtcaagtc caaggtgaat
     9421 ttgaccttat atttatagat gcagccaaag gtcaatataa gctatttttt gatttagtta
     9481 tagataaatt aaaagatggg ggacttttag tatctgataa tattttgtat aaaggtatgg
     9541 ttgctcatga tgattttgtt gtaagaagaa aaaagactat agttaaaaga atgagaaatt
     9601 atttagatta tatatgtaat tgtgactatt taagcacatc attggtacca ataggtgatg
     9661 gtgtagcact aagttataaa gaaagtaaaa gaggtgatgt agatggaatt aaataaagta
     9721 gaacttttag ctccagcagg tgatttggag agattgaaaa tagctataac atatggagca
     9781 gatgccgttt atataggtgg agaaattttt ggaatgaggt ctgcagcgaa gaattttagt
     9841 aaggaagata tggcagaggg agtagctttt gcacatgaaa gaggcaagaa agtattcgta
     9901 acagtaaata taatacctca taatgaagat tttctacaat tagaagacta tttgttggaa
     9961 ctagaagaga taggaataga tgcagttata atagctgacc caggagtgct tagtgtcatt
    10021 aaaaaagtaa ttccgaatat ggaaatacat ttaagtacac aagcaaatac aaccaattat
    10081 ctaagtgcaa acttctggta cgagcatgga ataaaaagag ttgtagtggc aagagaatta
    10141 tcttttgatg aaatatctga gataagagca aaaacacctt tagatatgga tatagaagcg
    10201 tttatgcatg gtgctatgtg tatttcttat tctggtagat gtttaataag caactatatg
    10261 actggaagag atgcaaacaa aggttcttgt gctcaatctt gtagatggca atatcatttg
    10321 gtggaagaaa aaagacctgg tgagtatttt ccaatatacg aagatgaaag gggtacattc
    10381 ttctttaact caaaagattt atgtatgata gagtatatac cagagttaat caaatctggt
    10441 ataacaagtt taaaaataga aggtagaatg aagactgcat actatgtagc tactgttgta
    10501 agagcataca gaatggcaat agatgaattt tacagagccc cagaaaactg gaagttcaat
    10561 ccaatgtgga tggaagaact aaaaaaaggt agtcatagac attttacatc aggtttttac
    10621 ttgaataaac caacaacaga agaccaaaat tatcaatcag catcatatgt tagaaactat
    10681 gattttatag gtatagttag agaaacagaa gatgaagatg gtcttattgt tgttgagcaa
    10741 agaaataaaa tgtgtgttgg tgatgaaata gaagttatgg gaccatataa agaaactatg
    10801 tttactaaaa ttgaagaaat gtacaatgaa gaaggagaag ctatagaatc agctcctcat
    10861 cctagacaaa tagtgaagtt aaagctatca gtaaaagttg gtaaagatta tatgcttaga
    10921 aaagttatag aagaaaaggt tgaagaataa taaaaaattg ataaaaatag tattagcccc
    10981 ttagaaattg tcaataatga aattatctaa ggggtgattt catgtcaaaa aagaagactc
    11041 catttttgaa aaaagtggga aagagaagct ggtgtatatt tactataatt ttaataattt
    11101 atagtgtttt aatttatagg ctagttgata tacaagtatt aaaaggtgat aaatacaaac
    11161 aaagtgttga atcacaaagc gttgagaaag tcgagttaaa tagtggaaga ggaataatct
    11221 atgatagaaa caacaagaag cttacagata caagtaaatc tcaggtatta attgttgaaa
    11281 aagaaaaatt aaataataac tataaaattt tggaacttat taagaaagct actaagatga
    11341 atgatttaga tatatataaa gctgtacaag agcaattgac acgccctata atacagattc
    11401 agactaaaaa tatcgataag agtatgaaaa aagaacttga gaaaaatgga ataatggtag
    11461 aagaaaaaac tatgaggtac gcaaaagatg gtttattatc acatacaata ggatatataa
    11521 aagaagatga taaatcaggt caatcaggaa ttgaaaagag tatggatagt gtacttagaa
    11581 attcaaatga aaagtatata agtgctttta aagctggaga tgcaggaaat gaaaaatctc
    11641 taaacatact aaagggaagt gtaaaaactg ttgataataa ggataaagat agacatctaa
    11701 aaactactat tgattataac attcaaaaaa aattagaaca aatactaaat aaagaagaaa
    11761 atcctacagc agcaataatc tcggaagcat ctacaggtga aattcttgca atgtgctcta
    11821 gacctaattt tgaccaaaat gacatatcaa aaagtttaaa aggaaaaaat ggggaatttg
    11881 aaaatagagt tataaaagcg acatatccac cagggtctgt atttaagatg gtagttttat
    11941 tttctgcact tgaaaatggt gttatagatg agaactatac ttacaactgt acaggtaaga
    12001 ctaaagttgg gaatactaat gaaatactgc gatgtaataa aagagatggt catggtttcc
    12061 aaaacttaag acaagctttt tctaattcat gcaatcctgc atttttagat atagctatga
    12121 aacttggaaa agaaaagata ctcaaatctg ctgaaaaatt acatttattt gaaaaggttg
    12181 atattggtct tgatgaagaa aaaattagag aagctccaaa aaacatatca ataagaaatt
    12241 tagctatagg tcaagaaaat atagaattta caccacttca aataaatcaa atgactcaaa
    12301 taatagctaa taatggaaca ttcaaaccac tatacttata taaaagtctt gtggacaata
    12361 atatgaatac aataaaaact tataagtctt caaaaaaaga agaacttatt tctccttatg
    12421 tgtgtacaca agttaaagaa tatatgaagt ctgtatctag gattggtaca gctaaagacc
    12481 taaaagatat tgaaggtggt tgtggagtta aaactggtac agctcaaagt agtttaaata
    12541 aaaaagctat agaccacgga tggataacag gattttatcc tgaagaaaga cctaagtatg
    12601 taataactgt gttagtagaa ggaactcaaa aaggaaataa atctgcaaca cctatattta
    12661 aggaaatatg tgaaagtata aaataaatta tgtactataa aatttaagga tatatcgtaa
    12721 ctttgatttt agatagaaaa ctttgtctaa tgatgtacga tatatccttt taaaatatag
    12781 tattaacttt cttgaacaag ctctttttct agttttttaa gagctttttt ttcgattcta
    12841 gatacatatg accttgatat atcgagtttt tgtgcaattt ctctttgtgt cttatagcca
    12901 taaggagtta acccatatct taactggaca atttctcttt ctctaggagt aagaattttg
    12961 tttagttgtt cgtaaagttt tccaacttga acttttagtt caacttcatc aagaacatag
    13021 ttagcctctg tgccaagtat atctaaaaga cttatttcat tacacactac tctttatatg
    13081 ttattggtat tactggtctt catgtaattt caaatgaacc attaattcaa aagaatctag
    13141 tttttgttct ttagacttat aataaattgc tttatcaatt acagatttta ggagtttatt
    13201 ttttccaagg gaatctgaat catgataaag tgataacaca gttttaatat cttctattat
    13261 tttagaattg tcttttttaa cagtactcaa tctatttttt actgtcttaa ttgaatcttt
    13321 aatttcttca gttcttgaac ttatattgtt ggacctttca ataaatgtat cttcatcata
    13381 tattccacgt tctaataaat catataactt atttttttgt ttacctaatt cttttaattc
    13441 agtttctaag ccctttaaga ctcgtttttg aaattctata ttactatctt tattattttt
    13501 atctaagtct aaatcacatg cttttaattg atattttttt accgtgtctt ccaaagattt
    13561 taaaatggca gcttcaataa attcaaatcg agaactttta ttgcaaccag gatggtaaca
    13621 aattatatag tcgtggtctg catatggtct atatactaat ggtctaccac aatttttgca
    13681 aatcactatt cctgcaagtg gattttttat ttgattacca tagctgacat gaccgttttt
    13741 tttaagtata ttttgagctt ttttccatgt tatttcactt attaaaggtt catgctttcc
    13801 ttttgcttca atccattcat ctttaggtct tagcttaaca gtttttattt tatttggatt
    13861 tttagatttt ttatagtcct ttttttgcca ctgaatgtag ccacaatata ccttattttt
    13921 tattatattt gtaattgcag aattgtacca attagcacct gtagcagttt taatgcctaa
    13981 gttatttagg tattttgata ttttactaca acccatatct tcattaatat ataagtcaaa
    14041 gatagtccta actacatcag catccttatt tggtacaagt attctttctt ttctacctgt
    14101 tgttacagca tcatatccaa atggagcaga agttcctatg aaattccctt cttcaactga
    14161 ttttattcta cctctttgca ttcttcttga tataagtttt aattcttttc ttgccataaa
    14221 tgcttcaaat tctgaatact cctcatcaaa ttcatctgtt aggtcgtatg tttttctagg
    14281 tgtgatgatt ttggttttac tttctttgaa tgtttctaat atgatacctt ggtctttcat
    14341 gtctccacga ccaagtctgt ctaagtccat tactaagact gcatcatatt tattttcttc
    14401 aacttctttt agaagttcaa gcattttggg tctatgtatt atactttcac cagaaactaa
    14461 ttcttctttt atctcaatta catctaagtt ttgttctttt gctagtttta gtagggttga
    14521 tttatgtcta cttaaagttt caaattcgcc ttgtttttct gcctcttcat cagcacggga
    14581 ttttcttaag tatatggcaa cactcataat aagtaatcac actccttatg cactattata
    14641 tagtaaatca atagagttta aaatatagaa aataagcatg tcaaaagtta atttaacttt
    14701 caacatactt attttctata atattaatgc cttataatcc tttaatatca tttagattag
    14761 tttttgcata ttcattatcc gggttaagtt caagcgcctt attgtaagat tctctagctt
    14821 tattaaattc tgatttactc ataaaaataa cacctcttaa attgtaagca tcactagcgt
    14881 ctattttata agcttcatta tttttatcta gatattctaa ggatttatca attgacttta
    14941 aagcctcatc atatcgttgt aactctatat aaatatatga ttgtgtataa tatgcaagtg
    15001 gatttttatc atcaatttct atagcttttt tgatatcaat agcagcttta ttataatcct
    15061 tatcttcatc tttttttgtt tctgacctag cgattttgac ataattagag ctaagagctc
    15121 ttttaaagtc tttatgccat aataatttat attcattatc ttcgattata aaatatctat
    15181 caaaatttgc agtatcttct ttattttcag tgatatactt tatagtatca gtttctttta
    15241 ttttaacaag atttgtgtac tctttattag aatatttgta tttctctact ttattttctt
    15301 caccaagttt ataatctttt agggtttgaa gtttaaataa agtatcttga tattcttcaa
    15361 atttttctag actgacactt tccttactgt ctttacttaa taaattatat gctacttcgt
    15421 agtctttgat ttctatacta tcaaggtact ttttagctac ttctgtagca ctttcttttg
    15481 gagcacatcc aaccattgat atcataatta aaaatgatac taatattgct gttatttttt
    15541 tactgattat cataatataa tcccccttat attgtctctc actaatttta ttttatcacc
    15601 atattctacc aaagtcatta atttaagaat gatatttttc attaatatta tattaagtaa
    15661 taataatttt taaaatttat ggtaacattt tcctgaaaag tattatataa tttaggtgaa
    15721 aatatttaag agaaggagat attatgaaaa ttaaaaaatt attaacaata gggttatctt
    15781 tatctatatt tattgctagt tgccctatat ctgcaaatgc tcttgataaa attgaatcaa
    15841 ttaaaggtgc agataaatac gaaactgctg gtataattgc agacaaacaa aactatacta
    15901 cagctatatt aattaatgca gacagtacaa tggctgatgg attaagtgca agtggacttg
    15961 caggagctat aaacgctcca attcttttga ctaagaaaaa taacatacca aatgctacac
    16021 ttaaaagatt agaaaaggca aaaaaagtat atataattgg aggagaaaac tctattaata
    16081 aatatactga aactgttcta aaaggcaaag gaatagagat taaaagactt caaggtagtg
    16141 atagaattaa gacaagctat aatgttgcaa aagaaataaa ttcaattaac aaagtaaata
    16201 aagtcatatt gactaatgct tttaaaggag agcctgatgc aatgagtgct gcacctgtag
    16261 cagttagaga taaagcagct atagtattaa ctgatggaaa aagtgttcca tttaatacaa
    16321 ctggtgtaga aagttatgta ataggtggta cttcatcaat gaatgacaaa ctagttaagg
    16381 atactaattc aactagatta ggtgggacag atagatatga tactaacaaa aagataataa
    16441 ataaatttta tagtggagta aaagaatttt atatagcaag tggtactgat ttagtgtatg
    16501 ctcttgttgg ttctaccatt gctaaaaata atgctattgt gttagttggt aatgatagta
    16561 ataaaagtgt attgaaaagt acaattaaat taactgctat aggaaatttg agtgatagta
    16621 tattacagca gtgtttaaat gtaacaaaaa atataggcga ttctaatact ggaagagaat
    16681 atacagtaga agatgctgct aatgcagtga aaaaactatt aggtgtgaag aaattttatt
    16741 acgaacctaa ttattcagaa gatgaagatt taatatttct actagataaa aattttgatt
    16801 tttcaggaaa agagtactat gtatttgctt acattaaaca gggtgcagag ggtgatgtta
    16861 gatactgtgt agctaaaaat gatatgagca aagtttatac atatgatgtt aatggtaata
    16921 tgaaagtatg taatccagat gattataaaa ataaaggaag tataacagag gaacaagcaa
    16981 aacagatagc attaaaagaa tgtgctaaga gacataaagt cagtatcaat aacttagtag
    17041 ttgatatgat agattttaga gatgatatat atagtggtaa tgtctatgct gttaaaatca
    17101 attcaaaaaa tagtaatggt acttttggtt ggttttttat agatatagaa actgggaaaa
    17161 tttataatta aaattagaac ttttaaacaa taattatata atttaccata atatgttata
    17221 atatttttag caagaagatt taacttacaa tctatagagt ggagttcatg tacaaaagat
    17281 tatcctccca acgattcgaa gggaggtgag ttcatggata actttttgtt taatgtttta
    17341 gctagtttaa cagctagtgt ggtagtttac ttaatcagta aactattcaa aaaagcaaaa
    17401 agccactctc gc
//
LOCUS       DAEPOZ010000059        17346 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.059, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000059 DAEPOZ010000000
VERSION     DAEPOZ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17346
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.059"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..716)
                     /locus_tag="KR440_001536"
     CDS             complement(<1..716)
                     /locus_tag="KR440_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0698592.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEEIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGESSLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNN"
     gene            complement(1154..2587)
                     /locus_tag="KR440_001537"
     CDS             complement(1154..2587)
                     /locus_tag="KR440_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBH0698593.1"
                     /translation="MVLRKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSREVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMTKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(2674..4590)
                     /locus_tag="KR440_001538"
     CDS             complement(2674..4590)
                     /locus_tag="KR440_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0698594.1"
                     /translation="MRRYEQLSKEIIENVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASIGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVIIIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGVLTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTSVVAAMYFKLKDKKIKELCIPAVISGICGVIEPAIYGITL
                     PRKTPFIYSCVGGAVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDSIVEISKDNNGQGVVEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTIMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKHFKSFVNQGDKIKKGQTLITFDIESISKEGYCLETPVVVTNYSDYVDIIENTQ
                     KHVNNSQELITVLA"
     gene            complement(4664..5524)
                     /locus_tag="KR440_001539"
     CDS             complement(4664..5524)
                     /locus_tag="KR440_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0698595.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKCIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(5875..7062)
                     /locus_tag="KR440_001540"
     CDS             complement(5875..7062)
                     /locus_tag="KR440_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH0698596.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGDNKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIESGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(7076..8467)
                     /locus_tag="KR440_001541"
     CDS             complement(7076..8467)
                     /locus_tag="KR440_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBH0698597.1"
                     /translation="MSSLKKKKFTMPQTLTIIFSLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIVPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQESMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLRL
                     EWYITEIAMIFTAIGIISGIASGLKQDEIVNSFISGAGDLITAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMVFLTIGLYLGF"
     gene            complement(9062..10762)
                     /locus_tag="KR440_001542"
     CDS             complement(9062..10762)
                     /locus_tag="KR440_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH0698598.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLASNGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(10734..12137)
                     /locus_tag="KR440_001543"
     CDS             complement(10734..12137)
                     /locus_tag="KR440_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH0698599.1"
                     /translation="MIVNKELIIDYLKSNQENIIKDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGENGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTDDKKDLVESLAYYTEIEELEEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGIYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSSAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(12401..12892)
                     /locus_tag="KR440_001544"
     CDS             complement(12401..12892)
                     /locus_tag="KR440_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0698600.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPNEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(12918..13730)
                     /locus_tag="KR440_001545"
     CDS             complement(12918..13730)
                     /locus_tag="KR440_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBH0698601.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEAMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLMGYEYKKVLSKEAIKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(13743..14894)
                     /locus_tag="KR440_001546"
     CDS             complement(13743..14894)
                     /locus_tag="KR440_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBH0698602.1"
                     /translation="MINFYQPSRINFGQGTLSELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLNLIEKGIEVVHFDRVEPNPTVELINEGFELAKKESVDVVLAVGGGSSIDTA
                     KVLALTFGLEKIDWDYMFSTYTNPFKIYDKVSEKELPLIAVSTTSGTGSQVTQAAVIS
                     RGSEKNTIFHQNCFSKECIVDPELMLTLPERITASTAFDAFTHAFESYINPNANDFTE
                     IMSEKSMELVINYLPKVMEDKNNIEYRTKLAVADTLGGSSLANAGAAAPHPLSEIIGG
                     VTHIPHGEALAIVFPKFAEKFYKDNIEKFAKVARMLNQELEKVEDDEAAKTLGNEIES
                     FLKRVGLKTKMSEFNVTKEQLEQITSCPVLGFLPFGKKEDLVEILEKSY"
     gene            15164..15805
                     /locus_tag="KR440_001547"
     CDS             15164..15805
                     /locus_tag="KR440_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBH0698603.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CTNLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTVDGANTFERDWSKVNK"
     gene            15831..>17346
                     /locus_tag="KR440_001548"
     CDS             15831..>17346
                     /locus_tag="KR440_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH0698604.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AVFELATVNKSYTELKEILFSNSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYS"
BASE COUNT         5578 a         3153 c         1884 g         6731 t
ORIGIN      
        1 ctattattgc aaataattat gtttccctta tctgatacca ttgccatacc taagtttgat
       61 aaatttatta tagaatttaa ttcatctgct tttatagtaa gttctttgta tttagtattt
      121 attcctgaat ataaactaat tatttcatct gtatacttaa ttagcctctc agtaatttct
      181 ttatcatcca gtttgagttt tgctattatt tgaataaacg ttgaactatc aatataccta
      241 tggcctaaat caataacttc ttctatatat tttggaacta aacttgactc tcctggcgtt
      301 atagctatct taatatttct atattcttca ttgggattgt atggtataaa attcaaatgg
      361 tttattccta tattgtataa attagatatt gtttccaata cagtatgctt attatcgttt
      421 actactaata catctatttc ttctggcaat gaaaataact tatatatatc cttttgcttt
      481 atgctcctat ttatagttat tatctttgag acatcttcta catatttttt tatttttaat
      541 actctatcat ctatcattac caatattatg tcatctttta ttaattcctt ctctgttatt
      601 ttatttaaat aataattttt tatcattact ttatctttaa atatcatttc tagattttta
      661 tttaagaaac attctaattt agacaaccca tcagttacta ctgctattgt tttcatttga
      721 ttactccttt taaaatatac tctactcttt tataatatac atctgatttt cactttaata
      781 gcacaaaaaa ttgtacattt agtatactaa catattcctt tattagtttt atgtccctaa
      841 atatcatagt tgcaatatct atcatatttc ttaaacttct ataaagcaaa aattgtgcca
      901 aattaataat tttcttaatt ttattctaaa gttcctcaat agtatttaca gacttagcac
      961 ttatagctta taagtcttta tattattaat ctatttttat aggttaattg tacaaatcat
     1021 atatcacttt tacctattaa atgcattata aaaaagatgg aataacgatt cgtcactcca
     1081 ccttttttgt aataaaaaat tgtatatata aacactattt actactcttc taagtacaaa
     1141 cagtatctac atactataac ctttcaccat tagattctat tacttgttta taccagtcaa
     1201 aactcttttt cttaatccta gacaagtctc cacttccatc atcatactta tccacgtata
     1261 taaacccata tctcttggtc atctcaccag tagatgcact tactaaatct atacatcccc
     1321 atggagtata tccaaataaa tcaacaccat cttctacagc ttccttcatt tgctctatat
     1381 gtgcttttaa atactctata cgatagtcat catttatcac tccattagat tcttttttat
     1441 cataagctcc taaaccattt tcaactacca tcataggtat tctatatcta tcataaattt
     1501 catttagtgc atatctaagc ccttcagggt ctatttgcca tccccattca ctagacttaa
     1561 gatatggatt tttcactcca cctaatatgt tgccacttgt attatcttct ttagaatcac
     1621 tacttacaca tgtagacata taataactaa atgtataaaa atctacacaa ccttctttta
     1681 aaatttctaa atctcctttt tccattttta tttcaatatt attttctcca aaatatctat
     1741 tcatataagt tggatattcg ccacgcactt gtacatctcc acagaaataa ttcattactc
     1801 tactatctct ttgagtttta actgcatcat ctggattaca agtataagga tacattgttg
     1861 caaatatttg catacatcca acttgataat ttgaatctat ttcatgagct aatttaacag
     1921 ccttagcact tgctacaaat tgatgatgta acccttgaaa acgaagttga ggtatatcta
     1981 cttggtttat aaaatctgta gtcccttcat ttaatattcc ttgtgataag tagcctccca
     2041 taggcattgt agctgaattt atttcattaa atgtcagcca atattttact tttcctttat
     2101 atcttttaaa tattgtttca cagtaattca tatacaagtc tattacctct cttgaaaccc
     2161 atccattgta tttctctgtt attgcaaatg gtatttcata gtgagatatt gttattaatg
     2221 gttctatatt atattttaaa cattcatcta tcacattttc ataaaattta agtcctgctt
     2281 catttggttc tttctccatt ccagttggaa atattctagt ccatgctatt gaaaatctaa
     2341 ataccttaaa tcccatttct gcaaataaag ctatatcttc tttgtatctg tgataaaaat
     2401 ctattgcttc atgacttgga tagaatgttc ctttttctat aactctcgtt attcttttac
     2461 ttactttttg agaaccccca gtacacatat ctgaacaaga aggacctttc ccatctaaat
     2521 tccatgctcc ttcacattga tttgctgctg ttgctccacc ccataaaaaa tcttttctaa
     2581 gtaccattac tctacctcca ataatttatt tatagtaaat ttattttaaa ataaaaatac
     2641 atatatctaa tttttaatta ataaaactct cttttaagct aaaacagtta tcaattcctg
     2701 actattattt acatgctttt gtgtattttc tataatatcc acataatcag aataattagt
     2761 aacaactact ggtgtttcta agcaatatcc ttcttttgat atgctttcta tatcaaaagt
     2821 tattaatgtc tgaccttttt taatcttatc cccttgatta acaaaagatt tgaaatgttt
     2881 accttccaac tgaatagtat ttaaccctat atgtatcaat agttcacaac cattatcaga
     2941 tataattcct attgcatgtt ttgttggaaa taaagtcatt atagtcccat caaatggtgc
     3001 cactactttt ccttctttag gtgttattgc tacacctttt cccaatatac ctgttgaaaa
     3061 cgctgcatct tgtatctgct ccaaagcctt tacttcacct tttattgggc tatatactac
     3121 ttctcttttg tttttttcta caacgccttg cccattatta tctttagaaa tttctactat
     3181 agaatcatct ttccaaaaaa ataatgttaa aacaaatcct actacaatag ctactattat
     3241 tgctattata gttgcataaa cacctttcat atctccagtt gttggattta tataattagg
     3301 tataccaaat attccaagtc cacccattac ataacttttt acattcatta tccccattac
     3361 agctcctgcc acagcaccac ctacacaact atatatgaat ggtgtttttc ttggtaaagt
     3421 tattccatat attgcaggct ctattacacc acatatccct gaaataactg caggtataca
     3481 cagctctttt atcttcttat cttttaattt aaagtacatt gctgctacta ctgatgtttg
     3541 tgcaaaagat ggtacaaatg ctcccactaa tattgtgtca aaccccatag tcataaggtt
     3601 tgacatagct attggtacta atccccaatg tagaccaaat attacaagca cttgccacaa
     3661 taaaccagtt aatacaccac ataaaattgg tgaaaagtta aataaagcta aaaatccatt
     3721 tccaactata ttagaagcat aggtagctat aggccctata accataaatc ccaatggtaa
     3781 agaaactaat aaaactgcca ttggtacaaa gaaattcttt actacatctg gtattatttt
     3841 agcaaacact ttctccaact tagctgctaa taatactatt ataattactg gtactactgt
     3901 tgaagtgtag ttcattgcta ttactggtaa tcctaagaaa gttatatata ctggtgattc
     3961 aaatattgtt ccagtaaata gattgtataa aggttgacct atagatgcta gagtatctaa
     4021 ttgaatacct gaatagcata aaactgcacc tataagcatt ccaataaatg gttttaattt
     4081 gaatttctta gcagctgtat atccaagaat tattggcata tactggaaca ttgcatctcc
     4141 gattgcattt aacataatat atgcaccatc agtttctcca tacaaaccca atgctgtaaa
     4201 tactgcatta aatcctttta tcataccagc agcacacaga actcctaata ttggttgaaa
     4261 aactcctgat attatgtcta taaacttatt aagtaatcct tttggcgcat ctgaatctga
     4321 attatctatt gtatctcctc ctaatccagc caaatcgcac acaacttcat aaacatctgg
     4381 aacatgatta ccaataacta cttggtattg acctgcactt ttcattacag taactatacc
     4441 atccattttt tttagcacat catcatttgc tattgattca tcttttaatt taaaacgtag
     4501 cctcgttata cagtgagata aactgctaat attatccttc cctcctacat tctcaatgat
     4561 ttcttttgat agttgttcat atcttctcat tttttctcct ccattttctt tttgaaatat
     4621 gaaggtttgg ccaacttaat gtcaccaccc aatatatata aacctaatta ttaggattta
     4681 tatctgactt aatactttta cttaccactc tttcaacgtg aatagttaag tataattttt
     4741 cctcatctgt taaatcgtag ttgtattttt tatgtataaa ttcatccaac ttttctatgc
     4801 atttataagc atttttatat ttaactttta ttacatttag taaatcatct tcattatttt
     4861 catgtaaatt attactaaca attcgttctg caaaatactt taagtgagta ataaatctat
     4921 gataataaac tgaatcttca tcaaatacta ttctgtaatg gtatttaacc atatttgaaa
     4981 tttcttgtat tatttgtgta atctcatata tagtttttat actttgatcc atttgtgcat
     5041 taactatatg cagtgctata aatccagctt catcttctga tagtctaatt tcaaattttt
     5101 tttctatata atccaatgct tctaacccta ttttaaattc actcttataa aatcttttta
     5161 tatcccaaag taatacattt tttacactta ctccttcacg agctctttct attgcagtat
     5221 acatatggtc tgttaaagaa atataaatac tatcatttaa tcttttccct agtttttctt
     5281 tagcatataa aattatttca tctgataatt ccatatatct tataggaata tctgctatta
     5341 gttctttaaa tttatcagat atatttgggt ctgaaagttt gaatatctta tcaattttat
     5401 cctttgatat atgctctcct gtacgttttt tataagctaa acctctcccc ataactacta
     5461 tttccttatg attttcatca gttgtaataa ttacattatt attgagtatt ttttcaataa
     5521 tcattgtgat gcccccttta ttagcattaa gttaaaataa atctttttta attatttaga
     5581 atatttaaaa tgtaatttat tacataaaaa aaaacctatt tattacacat taaatagact
     5641 cccatgagtc ctgagtaata aataggttta gcttgtattt aaacaatcac tatccgtttg
     5701 tattttattt taattattat ttattatatg ttagcaaatt tattttttag tgtcaacttt
     5761 ttctcaatat tttaattcta aataaattat ataaattgca tctgcaaagt actattttta
     5821 taaataaaca aaataaaaaa ggtattactt gctttaagta atacccattt atttttaatt
     5881 taagtggttt ttcttaatct catcaagtag tccatcttct aaataaattt ctaatgcaca
     5941 tcctaccatt gcctttactg ctttgtgaag tttatcataa gcttcctcag aatttgcata
     6001 atttagaaat tctttttcat gaacatatac cattggactt atatctaagg ctatttctgt
     6061 atacatagta gggcaaactt gacttacatt acctatatct gtagaaccta ctggaccatc
     6121 tttaccttct aatatatctg ttatacctga ctcaatcaaa ttattctttg ttatcttttg
     6181 aagtactttg ttatttatta tgttgtcaaa agaattttca aagtatctat aagaaagctt
     6241 agctcctgtc attaactctg caccttttgc acagtttata acttttttag tcacttcatc
     6301 taaataactt ctttcagcag ctcttacaaa aaacttacaa gctgcttttt ctggaactat
     6361 atttggagcc tctcctccat ttgaaactat tccatgtatt ctaacatctg attttacatg
     6421 ttgtcttagt gcatttatcc cagcaaaagt aagttgtact gcatctagtg cattaactcc
     6481 catatgaggg tgactagctg catgtgctga taccccttca aaactaaact ctaatgaatc
     6541 tattgccaaa gccttacagt ttatattatt aaaagcttct atatgcattt gataacttac
     6601 atcaacatca tcaaaagctc ctgccttaac taaatcacat tttcctcctg ttgtttcttc
     6661 agctggtgtt cctattagta caatttttcc tttaaaattt tcttttaact tacttaatac
     6721 tatacaagct cctagtgtag atgctgcaat ccagttatgc ccacaagcat gagctggttc
     6781 tttattgtct ccatatccag gaagtgcatc atactcagct aaaaatgcta tggtaggtcc
     6841 atcattatct cctaattcag ctctaaaagc agtctccata ttacaatatg gataaactgt
     6901 atcaaatcca tattccttca ttttctctac taagtatttt gatgatatgt attcttcttc
     6961 ccctaattct ggattgttaa atataaaatt acaaacttct tcaatttctt ttccaagttc
     7021 attctctatc tcataagctc tcttctttat actctctatc ataacttacc tcccactaga
     7081 atcctagata taatcctatt gtcaaaaata ccattacaag aattgttaat actaccatta
     7141 aaggaattac gaatctaacc cactttgacc aaggaatatt ggctactgct agtgctccca
     7201 ttaaaacacc tgatgtaggt gttattaagt tagttacacc tataccaaat tgataagctg
     7261 taactatcat ttgtcttgat acatctacta agtcgccaag tggtgcaagt acaggtattg
     7321 taagtgatgc aagccctgat gctgatggta ttaaaaatgc tattaatcct tctattaaga
     7381 aagttaaatt tataaatata gtttttggta atccatttaa taaatttgct gcagaattta
     7441 aaattgtatc tattataaat ccattttctg ctattattac gattccacgt gcaaatgcta
     7501 tacataaccc tgctgtaatt aaatctcctg ctccacttat aaatgaatta actatctcat
     7561 cttgtttaag ccctgatgct attcctgata ttatacctat agctgtaaat atcattgcaa
     7621 tttcagttat gtaccactct agtcttaaaa ctccatatat cataatcgcc atacctattg
     7681 tgaaaatggc tattaccatg gcttctcttt ttgtaaattc ctttatattt gtatcactat
     7741 taaccattga ttcttgattt agtgatatat cgtgtactaa agatttttta gggtcttttt
     7801 taactttatt ggcatacatc ataacaaatc ctatagatat agccatatat attataaaca
     7861 taacaactct aaactctatt ccagaacctg gtactatttg tgataatgct tgtgctattc
     7921 ctacagagaa aggattcgta gtagctgctc caaatccagc tgttgctcct ataaatactg
     7981 tggccactgc tgtcaatgaa tcatatccca tactggtcat aagtggaata aatatcatat
     8041 aaaaaggtag tgtctcttca ctcattccaa acgtcgtccc acctagtcca aatagaatca
     8101 ttgaaatcgg tattattaaa aattgaaact tcttcatttt atttacagta tgagctataa
     8161 cagcttctat agcccctgtt ttattaacta tcccaaaagc tcctccaact attaaaacaa
     8221 atccaactac ttcagctgca tctataaatc cgtttatagg tgctgtaaat acatcagtaa
     8281 ttccttgtgg atttgatggt gctttttcat aagttccagc tacaacaact gaccttccgt
     8341 ctatatctac tctttcaaaa tttccacttg gcactatcca agtaagaatt gccattaaca
     8401 ctattagcga aaatattatt gtaagtgtct gtggcatagt aaactttttc ttttttaaac
     8461 tgctcatttt taatctcccc actttttaaa ttaaatattt tattattgca tccctttatc
     8521 ctattttgta tagatgtaaa aaagctcaac atggatacat gttgagctaa atgtgctaaa
     8581 tacaacatac ctgcatcttc cctttatctt gtttaagata gctctccatc aaaattcggg
     8641 ataattttga tgacagtact gtacctatta aatacagtcc cagcactagc tttggacact
     8701 agcacttcgg cacttactcc ttttatattg cctcattggt ttccaacctc ttcaatatta
     8761 atcttagtaa atgcaacctc tacctcaatc tgttcgttag atgaggtaat tcttattaat
     8821 ttttattgat tcaaagtaat tgtagcataa tatgtcgaga ttgtaaatat tttcagtata
     8881 ataattctta ctttatgatg tcattccatt tttcacaaat aattttttct ccatataatt
     8941 taaatactgt agtatatttt aaatacacac aaactttcca caaattttta attattataa
     9001 tacctttata ataaaaaata aaagccatgt aaacttatca atttacacag ctttatatac
     9061 attaatctac tccaagtaca gctttaagct ttgatgctat tgtaataact cgtggaccaa
     9121 atataacttg aactgagttt ttctttttaa caactccact tgcacccaat tcatttaccc
     9181 agaaaccatc ttcatagaat ccatcaattg aatttagctc taccctaagt ctagttgcac
     9241 agtttgtaac attttttata ttattacttc ctcctaaacc ttctattata gaatgtacaa
     9301 gctttgtatc tttatcatct acttcctctt ctactgcatt gttgtatgct gttttaccag
     9361 catctacaga ttgtctttct ctatattctt tcttactata aagcttaact tcttctgaac
     9421 ttcttcctgg tgtttttata tcaaacttct taattgccca tgtaaatatt atataagtaa
     9481 ctataaaaaa tattggtatc aaaaataata atggtaacgc atccactttt tgtggttgta
     9541 ataaattagg aaacatattt ctaaatgatt ctcccattat agaaacatta aacatttctg
     9601 taaatacata cgtcaatcct gccagtggta catatgctac aaaatacaat aatggtgcaa
     9661 caaataggaa tgtatactct attggttccg ttacccctac caacatggct gttactattg
     9721 ctggtatcaa tatacttatt gcttttttct tattagcatt atcagccaac ttatacatag
     9781 ctaaagctgc tgctggtaat attgcaaaat gtattaataa acgacctgtt gtgaagttac
     9841 gagttatata agatagtgta tctgggtctc ccatttgagc taatcttata ttattaacac
     9901 cctcgtaaat tgctccacct acttccattg ttccacctac tcttgtatat tctataggga
     9961 atgcaattaa gtgatgtata ccaaatggta ataatgcttt atccattgct ccaaaagtaa
    10021 atgttcctaa taatccacta gaacctatcc aattaccaag tttagataat gccaacccta
    10081 ctggtggcca tataaagtac attgctgtac ccaatactat tgatgctata aacatcataa
    10141 tcattgaaaa tcttggacct gagaaaaaag ataatgcact aggaagttct atgtcaacaa
    10201 atttgttatg tactgcacta gctacaaatc cagctattat agctccaaat atactacaat
    10261 catatgaaaa gattcctaag aaagaaccat ataaagaact tcgactatat gcttctgcct
    10321 ctcctaatcc agatgccata aacgcttctg ctgtaactgt atctgaattt ataccatttg
    10381 atgctaacaa tgtacttata actgtgtgca ttcctataaa taaaacaatt cctcctaatg
    10441 caccccatcc tttttccttt ttagataatc caaaagctat acctgtaaca aagaatattg
    10501 gtaagtatct cattaccata aatcctaaat cgtttattac tttaaagaat acatttataa
    10561 atgacccttc ttttattagt ccgctaagcc ctaatgcaga aacgttttcc atatttgcaa
    10621 aagctgaccc taatcctata aatactcctg ctacaacaag taatataaca ggtatcatca
    10681 ttgctccaga aagtacttgt aatttatcct tcatagtatt atttcctcca tttttattga
    10741 aatgccaggc tatataatgc atatatatat acttctattg ctttgtataa tgtatctata
    10801 cttatatttt catttgcctg atgtgaattt ggattatctc cctctattgt gggtccaaat
    10861 gcaactccat tttttaacac tcttgcataa gatgcagctc cttttgtaaa aagttcggct
    10921 tcttttccaa aaccttgttt atatgcactt gataatttat ttattaattc actatctggt
    10981 tttacataca gtaattttaa atccttatat atatcaacta ttaaattttc ttttttccca
    11041 atttcattaa ttctattagt tataaactcc caacttatac ccttaggata tctaaagtct
    11101 attttcatat tcaattcatt tttctctaag aagatactca ttatacatat tgtagaaact
    11161 cccatatcta catcttcttt atatatccct aatttttttc catgattatc atctgtaaag
    11221 tatgaattta aaatatccct tattataacg cctttatcat ttagcttttt aattttctct
    11281 aaatcttttg ctaaactaaa tgcacagtta tatgacctgt gtggatttct cgataaagcc
    11341 ctttctcctg tatatcttac taatacttta ccttcttcta gttcttctat ctctgtataa
    11401 taagctaatg attctactaa atctttctta tcatcagttt taaaaacagc ctcaattttg
    11461 tcacatacaa atccatcttc tttgtttgac tttatatcaa ctaaattgtg atttctaaat
    11521 ttatctttat ctattttctt tcttagattg aagtataaaa taccattttc tccatttact
    11581 attgggaagt ttccatctgg agaaaatgca aattttggct gttcattgac actaaagtaa
    11641 tgctccatac attcccaagt cgtctcttca gctccaccca ctatcaatct aatctttctc
    11701 ttcggctttt cattaagttc tcttaagaaa agtaatgcat ataaaatccc tatcaaaggt
    11761 cctttgttat cattaactcc tctaccataa agatagttat ctttttgaca tactttaaaa
    11821 ggtttactct tccaaagttc tttattatat attggcacca catctatatg attaagcacc
    11881 cctactactt cttctccttc tccatattct atatgaattg catatccatc aaagtctttt
    11941 acaacaaaat ctttgtcttt tgctatttga ataagcttat cgaaggcatt tcttatttct
    12001 atcccaaatg gttgatttat atctgttgta ctttcatctc taattgaagg tatttcaact
    12061 aaacttctaa tatcctttat gatattttct tgattggatt ttaaataatc aataattagt
    12121 tctttgttta ctatcatctt aattatttat caccccttta tattaatttt acaaagcatt
    12181 tcagttcata tctacattat aaattttgaa tattattttt tcaatatttc aaatacttct
    12241 ctaaattacc ataatgatac ggtaaacaat caacagatta tgacaaaatc atactatacc
    12301 tattactaat attttaactt atctattacg tcttttttaa tgtaatttta ttccaaaaat
    12361 agatataaaa agatgtagcc ttagctacat ctttttatat ctattcaact tccattatta
    12421 tgctattaat atctacattt ccaggttcta tcctatcttt tactgttctc ttaacagaat
    12481 cagcatcatc cattataact acaggagtta ttatatttaa atcatttgac gttataaaat
    12541 ctaaatctac ttccattatt aaatctttag cttttacctt atctcctgcc tttaccttaa
    12601 catcaaacgg ctttccttct agtgacattg tctctaaacc tacatgtatt aatatatttg
    12661 tattatcatt actcttaatc attattgcat gctttgtatc aaatacattg actatttcac
    12721 catcaacagg agaatataac ttaccttcat ttggtattat tgctactcca tctcccatca
    12781 tcttttgggc aaaaactgca tctggaacat cttctaagtt cactaaattt cccttaaaag
    12841 ggctatatat tttctcaatc ttattttctt ttttcttaaa tatattaaac ataacacact
    12901 ctccatatta taaaatttta tgataatatt tttcttaaag cttttgctac accatcattt
    12961 acattagtat cagttacaaa atctgccata gattttactt catctgtagc atttcccata
    13021 gcaataccaa gacctgcaaa tttaatcatg gatatatcat tttcattatc acctatacaa
    13081 ataacttctt tgctatctat acctaatata tcacaaaatt ttttaactgc aatacctttt
    13141 gaagtacctt tactcataac ttcaaaattt atctttccag aacttactac ttctaaatct
    13201 tcttgttttt taaattcttc ctttgcttct ctcaaagaat ctacgttttc tgagaaaagt
    13261 atacatttta aaatttcatt atcagctttt tctaataaag tttcacaact tgcattttct
    13321 attatttcaa ttctgtcttc ttctttattt ttactattta ttaatctata tggatgttct
    13381 tcacctattt cattgttgga tataactatt ttacagcctt taaaatgttt gtttaaatta
    13441 tacttttcac ctattgtata gattttcttt atagcttctt tgctcaaaac cttcttatac
    13501 tcatatccca ttaatttaaa atatgtccca ttagttgata taatatatat atcatcccct
    13561 aaaacacttg taaaatattt catagcatta taaggtcttc ccgtaactaa ggctaccttg
    13621 ataccctttt ccatagcttc ttttagtgct ttaatatttt cttctgatac ttcaaatcct
    13681 ttccccatca aagtaccatc catatcagta caaattaatt tatatcgcat ttgtatcaca
    13741 tcctagtaag atttttctaa tatttcaaca aggtcttctt tctttccaaa aggtaaaaat
    13801 cctaaaactg gacagcttgt tatttgttct aattgttcct ttgttacatt aaactcagac
    13861 atttttgtct taagtcccac tctctttaaa aaactttcta tttcatttcc taaagtttta
    13921 gctgcttcat catcttccac tttttctaat tcttggttta gcattctcgc tactttggca
    13981 aatttctcta tattatcctt atagaacttt tctgcaaatt ttgggaacac tatagccaaa
    14041 gcctcaccat gaggaatatg tgtaactcct cctattattt cacttagtgg atgtggtgct
    14101 gctgctccag catttgccag tgaacttcct cctaatgtat ctgctactgc taactttgtt
    14161 ctgtattcta tattattttt atcttccatt actttaggta gatagttaat gactaattcc
    14221 atagattttt cagacattat ctctgtaaag tcattagcat ttggatttat ataactttca
    14281 aatgcatgag taaaagcatc aaatgctgtt gatgcagtaa ttctctctgg cagagttaac
    14341 ataagctctg ggtcaactat acattctttt gagaaacaat tttgatgaaa tattgtattt
    14401 ttctcgcttc ctctcgatat tactgctgct tgtgtaactt ggcttcctgt tccagaagta
    14461 gttgatacag ctattaatgg taattctttt tcacttacct tatcgtatat tttaaatgga
    14521 tttgtatatg tactaaacat ataatcccaa tctatttttt ctaaaccaaa agtcaatgct
    14581 aatacttttg cagtatctat acttgaacct cctccaacag ctaaaacaac atctacagat
    14641 tcttttttag ctaattcgaa cccttcattt attaattcta cagttgggtt tggctctact
    14701 ctatcaaagt gtacaacttc tatacctttt tctattaaat tcaatgtaac tcttttatat
    14761 aacttgtcta atggctctac atttggagta gtaactaaca aacatttctt tccatatttt
    14821 cctattattc taggtagttc acttaaagtt ccttgtccaa aatttattct tgatggttga
    14881 taaaagttaa tcatattttt cctcccttaa attattgact tcatattcaa attatacata
    14941 tcataggatt tttaaggcaa taccttcact gttaactctt atttactaaa gcttatggca
    15001 attattagac aataaatgtt ttattattac catatcatta tgtaaatttt attaagtacc
    15061 catgttataa ttttataatc aatcttattg tataattgac ttaataataa aaatagagta
    15121 tatacacttt tttatgacaa atagcttaaa ggagaattaa attatgaaat atttgataga
    15181 cagtgctaat cttgatgaaa ttcaaaagtg tttaaagtat ggatttaagg gaataacagc
    15241 taatccatct atgtatctta aagagaaggt aaacttccat gaatttattg agacatgtac
    15301 taatttaaat ccagaaatat taactgctga agtcattgga aataatttat acgaattaat
    15361 taattcatgt gataagatat taaatataaa taaaaacact ataataaaaa taaatttctc
    15421 tgaagttggc ttagaactta taaacatatt aaatagtaaa ggtgtaaaaa ctgcatgtac
    15481 cttgattttc aatgtaaatc aagcatcatt agctataaat gcaggtgctg attacctatt
    15541 cccttttata ggtagaaatg atgaaaatgg gaatgatggt ataaaaaacc ttacagaaat
    15601 atgtaacctt atatcaataa atggatataa tactaaagtt gttgcagctt ctattaaaaa
    15661 tgtattccac ttaactcaag catctctttg tggatgtgat tatgcagctg taacatatga
    15721 tttatttaaa aaagcttctt ttaaccagct tacagtagat ggtgccaata cttttgaaag
    15781 agattggtcc aaggttaata aatagtaact aagatttagg agggtttgca ttgaacaaaa
    15841 gattattaga tattgctgaa ttattactca acagctcaga ctatattact gtagatacta
    15901 ttgccgaaga attaaaagtt agtaataaaa ctattcgtaa tgaccttgtt attttagatg
    15961 aatggctttt agagtttaat ttatccctag ataaaaaaac tggttcaggg gttattattc
    16021 taggtaatga agatataaaa cttaaagtta ttagagatat caatgataaa tcaaattgta
    16081 tatatgcata ctctcctgaa gatagaaaaa ggtatattct aagtaaatta tttattaaaa
    16141 attctaagtt taggattcgt gatatttgca atgaactaca tgttagtaga gctactgttc
    16201 ataaagactt ggtagttatt caaaattttt tgcaaaactt taaagtgact ctagttagaa
    16261 aaaccaataa tggagtatat ttagagggaa aagagaaaga tattcgaaaa gcagtttttg
    16321 aacttgctac tgttaataaa agttatactg aacttaaaga aatattattc tcgaactcta
    16381 atgaatgcaa agatacccca tcaacaaaaa tatttaagga gctatttaac tatgattttg
    16441 agaaattatc tcaaatcact ctacacacct tacaagtaga aaaaagtaac cttagtgatg
    16501 agtattatat aaacttctta atacatatag ctatatgtat taagagaatt tcaattaaaa
    16561 gatatattaa cttatctgaa ggttttttta gagaattatt agaacatgaa tactttgaaa
    16621 aatcaaaaat tttatgtgct aacctaagtg aagcttttga cgtagaattt attgatgaag
    16681 aggtctgtta tatacttctt catatagaag gattacttaa atcaataaag aaaactacta
    16741 ctcttgaacc tcttaaaaat gaagaaatta cagagtataa tctatctgaa gcaatagctg
    16801 ttcattgggg aagtatacta aatttaaact taaaggatga tttaatatta ataaagtctc
    16861 ttgctaatca ccttaaatca gttctacaca gaataaacta tggatttact attacaaatc
    16921 ccatattaaa tgatataaag agaacatttc cttatactta taaggctgct aaagagtcta
    16981 atactgttat caaacagttg atgaattatg aagtcaatga tgacgaaata ggatatcttg
    17041 ctttatattt aatatcagca atagatagaa gcaaatctcc cctaaatact attttaatat
    17101 gtcactgtag tgatggtgta tctaatttat tagtccaaaa gttaagtttt gaatttaatc
    17161 agattaatat agttaaatct atacctctat cttctatttc atttacgaac tttgatgatg
    17221 tggacttaat actaaccact gctcctgtag actttgaaca taatgctgaa ctgataaata
    17281 taaatgctct tttaagtaag aatgatattt ccagacttag tacaattata aaaaaactat
    17341 attctg
//
LOCUS       DAEPOZ010000060        17259 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.060, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000060 DAEPOZ010000000
VERSION     DAEPOZ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17259
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.060"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(51..365)
                     /locus_tag="KR440_001549"
     CDS             complement(51..365)
                     /locus_tag="KR440_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBH0698605.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVNVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNEKLSVRLSKIEDSD
                     VN"
     gene            complement(389..817)
                     /locus_tag="KR440_001550"
     CDS             complement(389..817)
                     /locus_tag="KR440_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HBH0698606.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            complement(826..1494)
                     /locus_tag="KR440_001551"
     CDS             complement(826..1494)
                     /locus_tag="KR440_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBH0698607.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNSSSGFDMIMMTLLK
                     ALSQNNQQTSNGYNLSSNSENLFDELGSVVNNTASRFIDVDTKDKSVKSRIENAIEQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            complement(1533..2516)
                     /gene="rfbB"
                     /locus_tag="KR440_001552"
     CDS             complement(1533..2516)
                     /gene="rfbB"
                     /locus_tag="KR440_001552"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBH0698608.1"
                     /translation="MKKILITGGAGFIGSNFVHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDSSNYKFVKGDISNREQMYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSPINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKMILNLLDKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            complement(2554..3111)
                     /gene="rfbC"
                     /locus_tag="KR440_001553"
     CDS             complement(2554..3111)
                     /gene="rfbC"
                     /locus_tag="KR440_001553"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBH0698609.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFCDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVRYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFNYKCTNYYYPEFEHGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICIRR"
     gene            complement(3148..4029)
                     /gene="rfbA"
                     /locus_tag="KR440_001554"
     CDS             complement(3148..4029)
                     /gene="rfbA"
                     /locus_tag="KR440_001554"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBH0698610.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLKITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDMQYGR"
     gene            complement(4138..5010)
                     /gene="rfbD"
                     /locus_tag="KR440_001555"
     CDS             complement(4138..5010)
                     /gene="rfbD"
                     /locus_tag="KR440_001555"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBH0698611.1"
                     /translation="MKILITGSNGQLGKELVSQLEAINQSINQPKYVILATTRNDLDI
                     SNQTNIDNFILHNKPDVIVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LVHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENKEVNVVNDQFGTPTSTVDLAKIIIRIMETEHYGVYHATCEGE
                     CSWYDFAKKIFELKNIDIKVNPIKSNEFKCKAQRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            complement(5216..10297)
                     /locus_tag="KR440_001556"
                     /pseudo
     CDS             complement(5216..10297)
                     /locus_tag="KR440_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013725947.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
     gene            complement(10308..11840)
                     /gene="purH"
                     /locus_tag="KR440_001557"
     CDS             complement(10308..11840)
                     /gene="purH"
                     /locus_tag="KR440_001557"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBH0698612.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEVLKQKKNLRILKLAKIEDSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKAKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            complement(11833..12426)
                     /locus_tag="KR440_001558"
     CDS             complement(11833..12426)
                     /locus_tag="KR440_001558"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBH0698613.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            complement(12420..13496)
                     /locus_tag="KR440_001559"
     CDS             complement(12420..13496)
                     /locus_tag="KR440_001559"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBH0698614.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNAAYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            complement(13490..14857)
                     /gene="purF"
                     /locus_tag="KR440_001560"
     CDS             complement(13490..14857)
                     /gene="purF"
                     /locus_tag="KR440_001560"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBH0698615.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            complement(14869..15567)
                     /locus_tag="KR440_001561"
     CDS             complement(14869..15567)
                     /locus_tag="KR440_001561"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBH0698616.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            complement(15573..16049)
                     /gene="purE"
                     /locus_tag="KR440_001562"
     CDS             complement(15573..16049)
                     /gene="purE"
                     /locus_tag="KR440_001562"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBH0698617.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
     gene            16499..17011
                     /locus_tag="KR440_001563"
     CDS             16499..17011
                     /locus_tag="KR440_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH0698618.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKELSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVKQILDLPVKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
BASE COUNT         5205 a         3303 c         1826 g         6925 t
ORIGIN      
        1 cccttagcta aactcataca tcatagccta aagggatact atttaaaata ctaatttaca
       61 tctgaatctt ctattttaga taatcttaca gataacttct catttgtaat aatgctttct
      121 ccataagcaa acaatctatc atttgcatat atatctaatt tttcatccac attcttactt
      181 agttttatta tagagccttc ttttaaatct aatatatttt ttattgtttc tgttgtattt
      241 cctatagaaa cagctacatt tacttttgca ttaaaaacat tttgattttc ttctataaca
      301 ccagattttt cttctattaa ttgcccaaaa ttcaattcat atatatcctc ttttttacta
      361 ttcataaaac ttttctcctt gtataacctt atctatacat atctatcaac aagccttgta
      421 tcatatcaac agtagaaaca atatctatgt tttctttttc ttcactaatt aacttactag
      481 tcatatcctc aagtttacta tcaacaatct ttacattctt ataatactga tgctcccaaa
      541 agctactatt tttatcaagt gaatacatat tatctacaac agattttagg tattctttta
      601 ctgcattttt atatatacta atgtcgccat agttttgagt aagtacaagc ctttctcctg
      661 ccttttttac actttccaaa tttcgtttta tatcttccaa tgaagaagat tgttttaaag
      721 agtttaattt gtcagaaaat ttattactct cttttagttt tttaacctcc atatttatac
      781 ttggacttat gtttagctta tctatattta tccccataat aaagcctacc ctctataata
      841 tttcataacc ttaggaatat aattttgtgt ttcttttggc attttataca ggtcagagat
      901 tgattctact cctctacttt tcattcttcc aggaccacca ttatatgcca tcaatcccat
      961 ttctatgctc cctccaaaca tatcaatgta ttccttaata tgtctagttc cagcatctat
     1021 attctgctct atattaaaag ggtctgtaac ccccaagtct ctgcaatttt ctggcataag
     1081 ttgcataaga cccttagcac cagcagaaga aacagtattt ggattaaaat ccgattcaac
     1141 tttaattatt gcttttataa gattcgaatc cacattatat ttttttgatg cttgctctat
     1201 agcattttca attcttgatt ttacactttt atcttttgta tcaacatcta taaatctact
     1261 agctgtatta tttactacac tgccgagttc gtcaaataaa ttctcagaat tagacgataa
     1321 attgtagcca ttagaagtct gttgattatt ttgtgataat gcttttaaca aggtcatcat
     1381 aatcatatca aatccagatg aactattata tgtacagtta cattgatttg tagtattact
     1441 accagataat tgagtcaatg ctaatatatt aattagtgaa cttatctcat tcaatataaa
     1501 acgccccttt ctaaatttta taaatttcaa agttacactg ataaaacagc tttccaccaa
     1561 gattcatttt ctatatacca cttaacagtt tcttttatac ccaaatcaaa gtcatatttt
     1621 gccttccaac ctaactcatt ctctatttta gttgcatcaa tagcatatct taaatcatga
     1681 ccaggtctat catttacata ggatattaac tcttcagatt tatctaaaag atttaatatc
     1741 atttttacta cttctaaatt acttctttca ttatgacctc ctatattgta aatctcccct
     1801 atattccctt tatgtattat taagtctata gcagtacaat ggtcatatac atgtaaccaa
     1861 tctcttacat tttccccatt cccatatact ggcaattgtt tgttttccaa agcatttaaa
     1921 atcataagag gtatcaattt ttcaggaaaa tgatatggcc cataattgtt ggaacaccta
     1981 gatatagttg ttaacaatcc atatgttcta taataagaac ttaccaacaa atctgctgat
     2041 gctttagaag ctgaatatgg agaacttgga ttgataggag attgttctgt aaagaataaa
     2101 tcctttctat caattggtaa atcaccatac acttcgtctg tagatatttg atgatacctt
     2161 ttaactccat attttataga tgcatctaaa agtacttgtg ttcctataat gtttgtttta
     2221 ataaataaat caggattctc aactgaccta tctacatgag attcagcggc aaagttaaca
     2281 actatatcaa acctttcttc ttcaaatagc ttatacatct gttccctatt tgaaatatct
     2341 cctttaacaa atttataatt ggaactgtct tgtatttcta ttaatgtctc taaattacca
     2401 gcataagtca acaagtctag gttaacaata aaataatcta catacttgtt aagcatataa
     2461 tgtacaaagt tactcccaat aaatcctgct cctccagtta tcagtatttt tttcatattt
     2521 tttctcctaa atgttaattc taataaattt ttattaacgt cttatacaaa tatctttaaa
     2581 acttttttgt tttttgtcct tttctgataa aaatatttct tcaattcctt ttaaaggcca
     2641 ctcaatagat atttgcttgt cattccatat aaacccatgc tcaaattctg gataataata
     2701 gtttgtacat ttgtaattaa atactgcttt ttctgataaa actaaaaatc catgtgcaaa
     2761 cccttctgga atataaaatt gttttttatt cttatcagac aatattacac cttcccattt
     2821 cccaaatgtc tgtgaatctt ttcttaaatc aactgcaaca tcgaaaactt ctccctctgt
     2881 aactctgact aattttcctt gaggatatct aacctgaaaa tgcattcctc ttaaaacacc
     2941 ttttcttgag ctagactcat tatcttgaat aaaacacata tcaagaccct gctctataaa
     3001 atccttttta ttatagcttt ccataaaata tcctctttca tcacaaaata catttggttc
     3061 gactatatat aatccttcta ttttagtctt tataacattg atttttccca ttataacaaa
     3121 ctcccttaat aaaatttata ataaatttta tctaccatat tgcatatctt cactagccaa
     3181 ttttaataga tattgaccat attcagtttt tatcagtggc ttggctagtt tcattaattg
     3241 ttctctactt atatatcctt ttgtatatgc aatctcttct aaacatgcta tatacagtcc
     3301 ctgtcttgtt tgaaccactt caacatagtt agaagcatcc aatagacctt tatgtgtacc
     3361 agtatcaagc caagccatac ctcttcctag aagttccact tttagttttc ctcgttttag
     3421 atattcactg tttatagagg ttatttctaa ttcacctcta tctgatggtt caactagctt
     3481 tgctatctct acaacatcat tatcataaaa atataatcct ggtatagcat aattagattt
     3541 aggaactgta ggtttttctt ctattgataa tacattaaaa tccttatcaa attctacaac
     3601 tccaaacgaa tttggattac ttacatgata cccaaatata gttgctcctt ttcttttaac
     3661 tgcattctcc aacctttcac taaatccata tccataaaat atattatccc ccaatacaag
     3721 agacactctg tcatttccta taaattcttc tcctattata aacgcttctg caattccatt
     3781 tgctttctcc tgaattgcat atgttatttt aagccctagt gaacttccat ctccaaataa
     3841 ttcttcaaat gcttttatat ctcttggtgt agatattatt aatatatctt gtatccctgc
     3901 caacataaga accgacatag ggtaatatat cattggttta tcgtatattg gaagtgtttg
     3961 ttttgatata cattttgtta taggatatag tcttgtacca gaccctcctg ctaaaataat
     4021 tcctttcatc tttttccatc cttaaatttg tttttgtcat attatgatta tcggataaat
     4081 aaaagaaata tttatatttt ttataatatc ttattttctt ttctaaaaat aatacattta
     4141 cttttcattt atatcatatc tttctaaata ttcttcaatg gattcttccc actttctaaa
     4201 agaatttaaa ccaattaact tcaacataaa attatccaat acagaatata gtggtctttg
     4261 agccttacat ttaaattcgt tactcttaat tggatttact tttatatcta tattttttaa
     4321 ttcaaatatc tttttggcaa aatcatacca actacactca ccttcacatg tagcatgata
     4381 aactccataa tgttctgttt ccatgattct tattataatt tttgctaagt ctactgtact
     4441 tgttggggtt ccaaactggt cattaaccac attaacctct ttattttcta aagaaagttt
     4501 tatcatagtc tttacaaaat tattcccatt accatataac catgctgttc tcaatataaa
     4561 gtacttatgt gagaattgtt caacaaactt ttctcccata agtttacttt ttccatacac
     4621 agaattgggt tcagttttat tgtcttccct atatggatat ttagaaaaac cattgaatac
     4681 atagtcagtt gatatgtgaa caagcttagc attaactttt tctgatgcta ttgctaaatt
     4741 tctcactcct aaggcattta ttttatacgc aatttcgata ttatcttcac atgcatcaac
     4801 ctttgtatat gctgcacagt ttacaattac atcaggttta ttatgcaaaa taaaattatc
     4861 tatattagtc tgattggata tatccaaatc atttcttgtt gtagctaata taacgtattt
     4921 tggttgattt atactttgat tgattgcttc taattggcta accaattctt ttcctaattg
     4981 accattagac cctgttatta atattttcat taattatgtt gccctccaat aaatatttaa
     5041 ttttacttag taattaatta taacacagct tgtattttaa cttaggttga acttattatt
     5101 tcttagataa ataaaaaaga gatgtaatat taaatgatta tcatcatatt attacatctc
     5161 tttaaatcta aatatctaaa ttatatattc tagctatcaa ttaaacatca aagctttatc
     5221 taaaatattt aactccagat tcaaatatct tttggtcttt ttcacctata atattcttaa
     5281 atacttcctc accaattctc tcagaatgtc ccattttacc aaacactcta ccatcagcac
     5341 tagttatacc ttcaacagca tagaatgaac catttggatt aaactcaata tcataagtag
     5401 cttcattatt aaagtcaaca tattgagttg ctacttgtcc attagcaatt aattctctca
     5461 taacatcttc actagcaaca aatttacctt ctccatgaga aattgctata ttatgaatat
     5521 ctccaacatt agtttgagct aaccaaggag atttattaga tgaaattcta gttctagcaa
     5581 tcttagcttg atgacgacca atattattgt aagtaagtgt tggagcactt tcacttggaa
     5641 ctcttatttc tccatatgga actaatccta atttaattaa tacttggaat ccattacaga
     5701 tacctaacat tagtccatct ttttgagtta aaaattcatt tatagcttct tgcactctag
     5761 gatttctaaa tacagtagct ataaatttac cagaaccatc tggctcatca ccagcactaa
     5821 atccacctgg taacattatt atttgagaag attttatttc gtttactatt gtatctatag
     5881 aatcttctat gtctttataa gttaaattct tgaatactct tatgtttgca ttagctccta
     5941 ctctttcaaa tgctcttgca gagtcatatt cacagtttgt tccaggaaat gctggtataa
     6001 atactcttgg agtagctata cttaatgatg attttttagc ttctccttgt gatatataac
     6061 ttatagtctc tatctttgat tttacttctt ctgttttagt tgggaatata ggctctaaag
     6121 tactacagtg tgctttatat aattcatcta atgcaagttc ttcacctttg ataaatatac
     6181 tagcttcttt agttgtactt ccaagcacaa cataattgta tccttctaac atacataaat
     6241 catcatttgc taattcaagt actatatttc cataactagc ctcaaataat ttatcatctg
     6301 taaatgtttt ttcagcttct tcactaaact taaatcctat tttattacca aaagccattt
     6361 tacttattgc ttcacctaca cctgcaaatc ctaatgcata tgttgataaa actttatttc
     6421 catgtataag ttctcttact ttgtctaagt ttctctttaa ttcctcaaaa tctacaacat
     6481 cattttctgc cttgtttaca cataacatta ctacttttga gtctgctttt ttaaattctg
     6541 gagatactac tttcttcgca tctactgtat caacagcaaa tgaaactaat gttggtggaa
     6601 cttcgatatc tttaaatgta ccagacatac tgtcttttcc acctatagca ggtatctcaa
     6661 atttactttg tgcataaaat gctcctaaaa gtgctgagaa tggtttaccc catttttctg
     6721 gattgttacc aagtttttca aaatactctt ggaatgtaag tcttgttgta ctataatttc
     6781 ctccaatagc aaccaattta cacacagatt ctactactgc gtacaaggca ccatggaatg
     6841 gactccattt tcctaccttt ggattatatc cataagtcat tattgtagaa gtattagttt
     6901 ctcctccaag tactggtatc ttagcaacca taccttgtgt tggagtcgct tgatactttc
     6961 ctccaaatgg cataagtact gtattaccac caattgtatt atcaaacatc tctactaatc
     7021 ctttttgaga acaaacatta aggtcagata atacttttgt aaacttgtct ttcatacttg
     7081 cacacttaac atcttttact atttcattag agctaaagaa tgtattttct tcatctactt
     7141 tagtaacatg gacatcagta gtttgtttta ctccgtttgt atctaagaac tctctattta
     7201 tatctacaat tgcttttcca ttccagaaca ttcttaaata tcctgtatct gttactgtag
     7261 ctacatgtgt agcttctaag ttttcttcta ctgctaattg tataaattta tctttatttt
     7321 ctttttttat agctactgcc atacgctctt gtgactctga aattgcaagc tcagttccat
     7381 caagtccatc gtatttttta ggtactaagt ctaagtttat atctaaacta tcagcaatct
     7441 ctcctatagc aacacaaact ccacctgctc caaaatcatt acatcttttt atcatttgag
     7501 caacttcttt atttctaaag aatctttgta tttttctttc gttaggagca tctccttttt
     7561 gaacttctgc actacaagtt aagattgact cttcactatg ttctttagat gaaccagttg
     7621 cccctccaca accatctctt cctgtttttc ctccaagaag aactattaca tctcctgctt
     7681 caggtctttc tcttactaca ttctcttttg gtactgctgc tataactgca cctatttcca
     7741 ttctctttgc aacaaagttt tcatcatata cttctgcaac ttgtccagtt gtaagaccta
     7801 tttggttacc atatgaacta tatccatgag cagcttctgt agttattttt ctttgcataa
     7861 gttttccagg taaagtatct tctaaagtcg ttcttgggtc agcacttcct gtaacacgca
     7921 ttgcttgata aacataactt cttcctgata atgggtctct tatagctcca cctaaacaag
     7981 tagctgctcc accaaatggt tctatttcag ttggatggtt gtgagtttca tttttaaaca
     8041 ttactagata tttttccatc ttaccatcaa cttcaacatc tacgtttata ctacatgcat
     8101 ttatttcttc actttcatct aggtcattta atttcccatt cttctttaat tcttttactg
     8161 caacagtggc tatatccatt aaacatatat ctttatgtct gttcacatat acattgtctc
     8221 ttgagttaag atacatttga tacgcttctt ttactatatc attaaactta ccatcttcta
     8281 tctttacatt ttctatttca gtcataaaag tagtatgtct acaatgatca gaccaataag
     8341 tatctaatac ttttatttct gttatagttg gatttctctt ttctgtatct ttaaagtatt
     8401 tttgaacgtg ttgtaagtct cctaaagtca ttgctaatcc tttttcactt acaaatgttc
     8461 ttaatccatt ttcatctaaa tctataaatc catctaatac ttcaactgtt gttggtattt
     8521 cagtttccat ttttaatgtt tctggttttt ctaaagatgc ttctctacta tctactggat
     8581 ttatacagta atcttttatt ttagaaaact cttcatcagt tattttgcct gataatatat
     8641 aaacttttgc tgtattaata gcaggtctta tcccttgatt tacaatttgt acacattgag
     8701 ctgcccaatc acctctttgg tcgtattgac caggaagaaa ttctattgca aatactcttt
     8761 catcatttaa ttttggtatt tcttcataat atactacatc taaatttggt tcagagaata
     8821 ttgtctttgc tgcattttca taaacttctt ctgatattcc ttctacatca tatctattta
     8881 atattcttaa attttctata ttatctatgt gtaaactttc tactaagtct ttttttaaag
     8941 ctttagcctc aaggtcaaaa ccttctcttt tttcaactaa tactcttcta accattgagt
     9001 ctttattttc agtattcaac ataccttatc ctccatttta gaaaaattat cttcctatgt
     9061 ctgttctata gtgcattcct tcaaagttta tcttctttat gttttcataa actttttctg
     9121 ctgcatcctt aactgtagtt gactttgcag ttataccaat tactcttcct ccattggtaa
     9181 ctaaattacc atcaagcatc ttagtgccac tatggaatac tactatatca tcatccaagt
     9241 tttcaagacc agttataatt ttcccttttt cataactgtc tggataccct cctgatgtaa
     9301 gcataacaca tacagcttca tcatcactat agtttatttc tatatctttt aacttattat
     9361 ctagtatagt ttccattatc tcatgtaaat ctgtttctaa tctaaataaa acagattgtg
     9421 tctcagggtc tccaaatctt acattatatt caagtacttt ttctccatct tcagttatca
     9481 taagaccaac aaataaaata cctttaaaat taagtccatc ttttttgaag ccttctaaag
     9541 ttttttctaa tactgtctct tttactttat ttgctaattc ttctgtatat atttcactag
     9601 gtgaaaaagt tcccattccc ccagtgtttg gaccttgctc atagttgttt acttttttat
     9661 ggtcttttgc acttgccata ggaactattg tatcattgtc tacaaatgct agaattgaag
     9721 tttctattcc ttttaaaaac tcttcaatta ctattttatc tccagcagct ccaaacttct
     9781 tatctgacat catttctttt aaagttgcta tagcatcttc tctattttca ggtattacaa
     9841 caccttttcc agcagctaat ccgtccgctt ttattactac tggatatcca aaactatcta
     9901 tttctgatat agcttcttct aagtttgtat attctttata ttttgcagta ggtatgttat
     9961 gttttatcat aaattctttt gaaaaagctt tacttccttc aagttgagca cattttttat
    10021 taggtccaaa tactcttaat ccttcttttt caaactcatc tactattccc ataactagtg
    10081 gtacttctgg tcctactatt gtcagaccta tttcattttc tttggcaaat tttaaaagtt
    10141 cttctatatt agtatcacct atatttacac attctgctac atttgtaatt cctgcattac
    10201 ctggcgcgca ataaactttt tctacatttt tttctttgct tagtttccag catatagcat
    10261 gctctcttcc gccaccgcct acaaccaata tcttcataat atcctcctta gtgtttgaag
    10321 tgtcttattc ctgtaaatac cataaccata tcatacttat cacaagcttc tattgaatct
    10381 ttatccttta tagagccacc tggttgaact actgctgata ttccatattc atgagctaat
    10441 gttacacaat catcaaatgg gaaaaatgca tctgacgcta aaacagctcc tgtaaaatct
    10501 ttatctttgt tattttcaag agcatttttt aatgcccaaa ttcttgatgt ttgtccacaa
    10561 cctaaagcta aagtttgacc attttttact atcgcaattg catttgattt catatttttt
    10621 actactctca taccaaattc catgtcctta agttgtgcat ctgttggttt tgcttttgtt
    10681 acattttcac ttttttctgc taatatatta tttctatctt gtattaataa ttttccatct
    10741 aaatatttca tttcatatgg ttgtaaacta tcttctatct tagctaattt taatattctt
    10801 aaatttttct tttgtttaag tacctctaaa gcttctggct caaaatcata tgctactact
    10861 atctctaaga atatttcatt taatttctct gctgtagctt tatctatagt agaagttatt
    10921 cctactattc ctccaaatat tgaaacctta tccgcttcat aacattttgt atatgcttca
    10981 aaagcatctt ttcctagacc taccccacat gaattagcat gttttatagc tactgaaact
    11041 acttcttcac tatctttaaa ttctctcatt acttctaaac atccatgtaa atcatttaaa
    11101 ttgttaaaag aaagttcttt accgcctaat tgctcataat ttaagattgg attttttgca
    11161 tttgattgtg aatataagaa accattttga tgtggatttt ctccatatct taaagtttgt
    11221 tctttttgga aagttaaatt taatatgtca ggatatgtat ctccaacttc tccagcaaaa
    11281 taactagata ttaacgcatc atatcttcct gtagttgaga aagctttata tgataatttt
    11341 tttctgtctt ctaatgtcat tgtatctgtt tttaattttt ctattataga atcataatct
    11401 tttatatcta ctactactat tacatcttta tagtttttgg cagcagaacg tatcatagat
    11461 ggtccaccaa tatctatatt ttcaattatt tcatcatgac tttttccagc ttttaaagtt
    11521 ccttcaaaat catatagatt tacagctact aaatctattg aatgtatttt atgctcattc
    11581 acagtttcta catgagattc tttatctctt ttataaagta taccaccatg tatatatgga
    11641 tttaatgttt ttactctccc atctaatatt tctggaaaat ttgtaacatc ttcaacttgc
    11701 attacattaa caccattttc ttttaatgtt ttaaatgtat ttcctgtaga tataatttca
    11761 taatctaact tatttaactc cttagcaaat tccactacac cagtcttgtc agttacactt
    11821 attaaagctc tcttactcat ttataaacac tctcctacct tgaagtttta atttattttc
    11881 acaaaaaaga cttatacttt cttttaaaat cctatgttca acttctaaca ctctttttgc
    11941 aagtgtcttt gcatcatcat cttgatttac cttaactaca tcttgcatta tgattggtcc
    12001 agtatcagca ccttcatcaa caaaatgaac tgtagcacca gttactttag ctccgtaatc
    12061 tataacacct tgatgaactt tttctccata gaacccagct ccacaaaatg aaggaattaa
    12121 tgatggatgt atgtttatca ttttgtttct aaactcatta accagctttg ggctgattat
    12181 ttttaaataa ccagccaaaa ctactaaatc tattttattt tccttaagaa tctctattat
    12241 cttgtcttca tcagtttcac aaattgcttt tatgttatga tttttagctc tctctagccc
    12301 ataagcacct tgcttacttg agataactac tttaacctga ccttttattt ctccagactc
    12361 agtgccatct ataactgctt gtaaatttgt tcctccacca gatattaaaa ctcctatatt
    12421 tagcataatt ctacaccttc atgactatct actacttcac caattatata cgctttatct
    12481 tctaataatt cactgtattt tttatctaca taagcagcat tgtcattttc tctattattt
    12541 ataaagttta caacatcatc agcattttct ttatctacta ttaaaactag cccaataccc
    12601 atgttaaaac tcttatgaag ttctctctca tcaacagcat taaaaccttc tatcatttta
    12661 aatataggag gcttctccca agatttttta ttgatatcta gtcctaaccc tttaggtata
    12721 actcttgtta tattttctat aagccctcca ccagttatgt gagctatagc ttttatgtca
    12781 tgtttagcta atacatctaa agctaattta acatatattt tagttggtgt taaaagtgct
    12841 tctccaactg tcattcctag ttcttcaaca tattgttcca ttttataatt gtaagtttct
    12901 aaaaatatct ttcttataaa agaaaatcca ttgctgtgta ctccacttga agatatacca
    12961 accaatacat ctcctgattt aacattgttt ccagaaacaa tcttttcttt gtctgctatt
    13021 cctacagaaa atcctgctaa atcatagtca tcttctccat acattccagg catttcagca
    13081 gtttctccac caattagtgc acaaccagac attttacaac catctgcaat tccaccaact
    13141 attttttcga tatgctcagg cactaatttc ccaagagcta tatagtcaag gaaaaataat
    13201 ggttttgctc cttgacaaat taaatcattc acgcacattg caactaaatc tattccaaca
    13261 gtatcatgct tgtccatcat ctgagctatt tttaacttag tcccaacacc atctgtagat
    13321 gcaagcaaaa ctggttcttt catacctaca aaatctttta aactgtataa tccactgaag
    13381 ttacctaaat ctccaactac atttccatca taagttcctt ttattttacc ttttataagg
    13441 tctactgctc tatttccttc atctatatct acacctgatt ctttatatgt taacatgaaa
    13501 gtagttcctc cttatcaatt ttcttaactg ggtattctcc atcaaaacaa gctttacaga
    13561 aagtagactt atgttctgtt gcatctaaca ttccttctat atctaggaat tttaaactat
    13621 cacatccaat atattctctc atttcttcta cgttgttact tgatgcaatt aatttagagc
    13681 gatttggagt atctattcca tagtaacaag agtaagctac tggaggagaa gttattctaa
    13741 gatgaatttc ttttgctcct gcttctctta aagattttac taattgttta gatgtagttc
    13801 ctcttacaat tgagtcgtct actaaaatta tagattttcc ttttattatt gttgaaagag
    13861 gatttaattt tatttttact gctatttctc tttcttcttg tgtaggtttt ataaatgttc
    13921 ttcctacata tctattttta actaagcctt cacttatttt taatccacta gcatttgcat
    13981 aaccaatagc tcctggccaa cctgaatctg gaactggaac taccatatct gcctttacag
    14041 tttcattctg agctaatctc tcaccacatt taattctaaa ttcatatgca tttacattgt
    14101 ctatagttgc atcatttctt gcaaagtata tgtgttcaaa tatacaactt ttcttaactg
    14161 gtttataatt ttctgaataa aaatatgatt ttagttctcc atctttgact actattatct
    14221 ctcctggctc tacatctctt ataacttctc cgccaagtat atctattgca caattttctg
    14281 atgcaaatat atattcatca ccctttttac caagtacaag aggtctgaat ccatgtgggt
    14341 ctctaactgc aaccaattct tcttgactca ttattacaag agcatatgca ccttttatat
    14401 aatccattgt cacttttaaa ctttctacta tatccccttt gtaatatcta gcaagtatat
    14461 ataaaatcac ttctgtatct gaattagttt ggaacatata tccatcttct tctagcatat
    14521 cttttaaata atttgcatta actagatttc cattatgagc tatggctaat tgtctttttc
    14581 tacaacttcc aactaaaggt tgacagttag aaacatgact tcctccagct gttgaatatc
    14641 taacatgtcc aatccctata tttcctttta acttagataa ttcattttcc ttaaaaacat
    14701 ctccaactaa tcccatatct tttttgtact taatttctcc atcatctaaa agagctaaac
    14761 cacagctttc ttgccccctg tgttgcatag aatataaaga ataatatagt tctttagtta
    14821 catctttatt agagtaaatc cctaaaactc cacacataca tttgcctcct atttattatt
    14881 cattcttgaa agtacttccg tataaccttc tacaaggtct ccaaggtctt ttctaaatct
    14941 gtccttatct aacttttcat ttgtatttac atcccaaagt ctacaagtgt ctggtgatac
    15001 ttcatctgcc aatattatat ttccttcaga atccttacca aattctattt taaagtcaac
    15061 taactttagg tttaatttta agaaaaatgc ttttagtaac tcgtttattt ttaaagtttc
    15121 ttctcttaag aattttaact cttctctagt tgctaacttc attgccactg cataatcatc
    15181 atttagcatt gggtcaccat aagcatcatt tttgtaactt atttcaaaaa ttggctcatc
    15241 gaaaactaca ccttcttcaa gaccaactct tttacagata gaaccagcag caatatttct
    15301 tactattact tctaatggta aaatctctac ttttttaact aacatttctc tatctgataa
    15361 actttttata aagtgagtat ttatattatt ttcttttaac atttcaaaga ttattgttga
    15421 tatcttatta tttagtatac cttttgaaga tatttctgct tttttttctc cattaaatgc
    15481 tgttgcatca tctttataat aaacaacata ttcattttca ttatctgtag aatatacttg
    15541 ttttgctttt ccttcatata ataacatttc tgctaccccc ttaaattctt gtcgtcttct
    15601 aacacctttt gtgccatctc ttctctatat gatttcaaat cctcttttaa ttttgggtat
    15661 tttaaagtca ttatttgtaa tgccaataaa gctgcgttta aacctaagtc tattgtaact
    15721 gttgcaactg ggattccttt tggcatttga actattgata aaagtgagtc tagtccatcc
    15781 atagttgatg atttaatagg taatcctatt acaggaatta atgtttgtga agctattaca
    15841 cctggtaagt gtgctgcttt tcctgctgct gctattatta catcagtatc atcttctatt
    15901 tcttttagga atattgaaag ttgttctggt gttctatgag ctgataatgc tcttgctaca
    15961 acctctattc catatttttc aagcagtttt atcccttctt ctaattttgg ataatctgat
    16021 tttgaaccca taactactgc tactttcatt agacttcccc tctccgttta taagttattt
    16081 tacactacaa atcattcgta ttattatacg aattatacac cttaaatttt atattaatat
    16141 acatatatta tcaactcttt tttgaaattt tttaaaaaaa atatttcttg tatttttttc
    16201 attcgctacg tatcgaactt tatttatgtt tttttgttaa aataaaaaac agattatctc
    16261 aaaatcggtt ttctaattaa attttaagct caatactctt ttttaaatct tgtatattat
    16321 tttttattac ttctttttta attaatatct tggatttata gtcttgtttt ttagttctga
    16381 atgttacttt tacttagata aaaccaacac aaagtttatt ctttatataa gtaatagcta
    16441 aaacttaaag taatgatatt atataaatag aaataatttt atttaaagga gaatctgtat
    16501 gttagaagtt ataaaaaata gacgttcaat aagaacatat atagataaaa atatagaaga
    16561 agataaaata actgaaatac taaaatcagc tatgcaagca ccttcatcaa aaaatgctca
    16621 accttgggaa tttattatag tagatgataa ggaactgctg aaagagctct caaagtcaca
    16681 gcatagagca aagcatatag aatttgcacc attatgtata gtagtgcttg gaaatagaga
    16741 taaattctta aaacctggta aatggataca agatttaggt gcttgcacac aaaatttatt
    16801 attagaagtt actaatcaag gtctagctgc ttgttgggca ggagtatttc ctaaaaataa
    16861 agtagtaaat aaagttaaac aaattttaga cttaccagta aaattagttc cttatgcact
    16921 tatatctatt ggatatagtg aagaaaaaaa tgaatttata gaccgatttg atgaaaataa
    16981 aatacataga aatgtatata aaaatagata atgtttagta atataaatga aaatacatag
    17041 taaaaatgcc tttagaatac atttgtattc taaaggcatt aattttgact gaacacctaa
    17101 ttagcctcta aggtaaatct taactatcat ttattattta tataccaatt gaatattata
    17161 tccttcaata tttttattta tttcaaatat attaagaata tcctacaatt aaagttagct
    17221 atttttcatt aaagtatttt agtaaattaa taatgataa
//
LOCUS       DAEPOZ010000061        17143 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.061, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000061 DAEPOZ010000000
VERSION     DAEPOZ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17143
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.061"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            126..710
                     /locus_tag="KR440_001564"
     CDS             126..710
                     /locus_tag="KR440_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HBH0698619.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(814..1593)
                     /locus_tag="KR440_001565"
     CDS             complement(814..1593)
                     /locus_tag="KR440_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBH0698620.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(1663..2919)
                     /locus_tag="KR440_001566"
     CDS             complement(1663..2919)
                     /locus_tag="KR440_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBH0698621.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGVAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            3177..4076
                     /locus_tag="KR440_001567"
     CDS             3177..4076
                     /locus_tag="KR440_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBH0698622.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            4100..5548
                     /gene="ddlR"
                     /locus_tag="KR440_001568"
     CDS             4100..5548
                     /gene="ddlR"
                     /locus_tag="KR440_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HBH0698623.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDMEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            5607..6413
                     /locus_tag="KR440_001569"
     CDS             5607..6413
                     /locus_tag="KR440_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBH0698624.1"
                     /translation="MNLILGDNMSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSN
                     PNKKGNIKSQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     IKLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDV
                     TPKISTVYADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            6439..8358
                     /locus_tag="KR440_001570"
     CDS             6439..8358
                     /locus_tag="KR440_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071223118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBH0698625.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGLIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVKRGAQ
                     ARSTKQKARLQRFDELMNRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKRESVKKDDSTTLKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(8600..8947)
                     /locus_tag="KR440_001571"
     CDS             complement(8600..8947)
                     /locus_tag="KR440_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HBH0698626.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENSVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(8934..10862)
                     /locus_tag="KR440_001572"
     CDS             complement(8934..10862)
                     /locus_tag="KR440_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HBH0698627.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFFEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTTLPIVLSKVKNNEQE"
     gene            complement(11273..13462)
                     /locus_tag="KR440_001573"
     CDS             complement(11273..13462)
                     /locus_tag="KR440_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HBH0698628.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEVPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKLGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEYNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDMYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKKALPE"
     gene            13793..14524
                     /locus_tag="KR440_001574"
     CDS             13793..14524
                     /locus_tag="KR440_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBH0698629.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLYMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQNNKECKIPTLEEVLILISPTD
                     LILNIELKTDKINYPNIEKDVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSVKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
     gene            14604..15239
                     /locus_tag="KR440_001575"
     CDS             14604..15239
                     /locus_tag="KR440_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBH0698630.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNIHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(15490..16083)
                     /locus_tag="KR440_001576"
     CDS             complement(15490..16083)
                     /locus_tag="KR440_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HBH0698631.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(16154..17017)
                     /locus_tag="KR440_001577"
     CDS             complement(16154..17017)
                     /locus_tag="KR440_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBH0698632.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWDKFTYKSLVNALEFKDDLYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
BASE COUNT         6213 a         2613 c         2120 g         6197 t
ORIGIN      
        1 tggttgaaat tatttaaata aatactgttg acttagtttc atatatttac cattaaactt
       61 ttatcgtaaa tatcatatca taataatata tttaataagt ctaataaact atacgaggtg
      121 taaatatgtt aatgaatacc cattttatta tggcaaaatc aatacttgat aatatagatg
      181 aaaataaaac attctttata agcgaaaaaa attttatata tggcaatata aagcctgatg
      241 ctttttctaa atataaatta aaaaaacatt atttagatga gtcatttaat atgattgttt
      301 ctaaaataaa ctatctatgc agtcttaata tagatagtct ttctaaatac ttttcagtaa
      361 gtagacttag tcaggaacta ggagtcatat gtcatttttt atgtgatttt ttctgtgtag
      421 ctcacagtga acgttgggaa tttaagcata gcttaaataa acatgttact tatgaaagag
      481 aacttggagc atttgcaaaa gatgttgatt tatctaaaat taaatgtggt actataaata
      541 gtagttttga gaagtttttt actaaacttt atagtgatta caaaaagaaa actgattata
      601 tgaatgattt aaaattttca gcttatactt gtaatagtgt cataaattat atacttgact
      661 ctattttatc taatacaata aaatcatatc atatagcaaa ttgcggttaa cttaagaatt
      721 tttaaattta tataaactta taattgaata taattaaaat gactgctcta tagaatataa
      781 ttttatactt ctctaggaca gtcatttttt atactaacta tttatctcca ttataacatc
      841 tatggcactt tttacactat ctactacata atttgcactt tgctttacat cttcccttgc
      901 cttagacatt gcaaaactat atttaaatcc ttctaacatg caaatatcat ttggagaatc
      961 cccaatagta gcaacttcat ctggacttac atttaacgtt tttattacat gctctatact
     1021 tcctcttttt gacacacttt taggaactat atttaaagta tatgttccag aaaaatatat
     1081 ttctaatact tcactaaact cactttttag ataaagttct attctggata actcttccaa
     1141 attttcagaa catacgccta tatttcctat aagattatca aaactacttc ttttttcaaa
     1201 atctgggtca actataaagt cactagaata atgtctaaaa gcagtggtat cactacccac
     1261 aattacatca cccttatatt taaaataaag ttgtgcatca cttgattcaa atctatcaat
     1321 gacttttttt actaaattat catctatcgt tcttttaaaa attacattat aatctttatc
     1381 atatataaat gaaccatttt cacatacata atatccattc atatctaaac tatctttgat
     1441 aatattatgt gcttgtttaa aaattctacc tgtagcaaca ttaaactgta ctcccttttt
     1501 ttctatttct tcaattgcta ctatatcttc ttttgttatg gttccatttt catatagagt
     1561 tccatctaaa tcacaaaata tatgttttat cataaattaa aagcctccaa aaacaatttt
     1621 atattatagt aacacttcaa atatatccaa tatcataaca acttaaagct tttctaattg
     1681 tattctcaat tcattaagta aatctctagt agctcttaat cttatctttc ttctatcccc
     1741 attaaatata tttttcttta ctattgtttt tccatttata tatattgcag tataaactag
     1801 tcccacaggc ttctctttgg ttcctccacc aggtccagct actccagttg tggatatacc
     1861 tatattagct ttaagtcctt tagcaacgcc ctcagccatt tctatagctg tttgctcact
     1921 tactgctcca aactcttcca aagtttctct ttttactcct aatcttttca ttttagaatc
     1981 attagaatat gttacacatc cttcaagaaa tacagaagat attcctggat agtttataag
     2041 agatgaagaa accaaacctc cagtacaaga ttcagccact gctattgtta gatttttttc
     2101 tacaagtaac ttagaaacag catcttctaa agcagtctcc ccttcagata tggaaatatc
     2161 tccttcagta tatacatatt taccaactct actttttatt ttttcttcta ctggttttat
     2221 taatttaaaa gcttctcttt cattttcagc ctttgcagtt attcttattg taacttctaa
     2281 ttcttttgca tataaagcaa ctgttggatt agtctgctca ttaattatat cgagaatttc
     2341 taattctaga tttgactcac ctattccata taatcttaaa gtcttagata ctagcatttc
     2401 atttgtgaat tgttgtaagt aaggtttgac actctcatta aacatagctt tcatttctct
     2461 tggaggtcca ggcaatacta ttataaattt accatctttt tttaaaattg cccctggagc
     2521 tgtaccatta ttatttttta gtactatagc atcttttgga aagtacactt gtttcatatt
     2581 attttcggcc attttttttc ctgttttaac aaagaaagat tccaaaacct ctagtgaagg
     2641 tttatgtagt tctaatttct gaccaaagta ttctgcagca gtttcttttg tcatatcatc
     2701 accagttggc ccaagaccac ctgtagtaat tacaaaatca cttctcttta agctttcatc
     2761 aaaacattct aaaagtcttt gtttgttatc tccaacagta ctctgatggt atacttctat
     2821 tcccaatgat gcaagttctt tagctaaaaa ttgagaattt gtatttacta tatctcccaa
     2881 taatatttct gttcctacac ttattatttc agctttcaaa atttatcact ccttgattta
     2941 tagccattaa gactagtctt aagttcataa caataatttt agcaaaaata ataacataaa
     3001 taaagttaaa aaatcattta aacatatctt taaactagat tattattcta aaaccacaca
     3061 taatataaac ttcgtatata gtggtacttt catcctcaac tgttaattca tataattaag
     3121 tatgtaaata aaaagtttac tttaaaataa taaaaattaa aataggagtg tataatatga
     3181 aaatagcagt tataatgggt ggaatatcat cagagagaga agtttcttta aattcaggaa
     3241 aagagatata taataacctt gataaaaata aatatgaagt tgtaaaagta ataatagatg
     3301 ataaaaaaga tatatttaca aagataccag aagatataga ttttgcaata cttgctttac
     3361 atggaaaatt tggtgaagat ggatgtattc aatcaatact tgagactatg gatataccat
     3421 actctggatg tggacctctt tgcagtggga tgtgtatgga taaaaatata actaaaaaaa
     3481 tgcttagaga ttcaaaccta cctactgctc catgggtttt agtaaaatca gttgatgaaa
     3541 tcgactatga tgaaatagat aatataggtt atccagtatt tataaaacca aactcaggag
     3601 gttcaagtgt agccacattc tttattcatt ctaaggatga agttgaagaa gcagttagaa
     3661 aaggtctgga agttgacgaa tttgtaatga ttgaaaaata tataccaggt ggagaatata
     3721 cttcattcat attaaatgga gaagtgttcc ctacaatatc tataaaatct gattcaggat
     3781 tctttgatta tgaagctaaa tactcagttg aaaaaggtgc aaaggaagaa gttgtttact
     3841 tagatgaaga acttcaaaaa agagtaaacg aaatttctga gacttgttgg aaaattttta
     3901 attgcaaagc atatgtaaga gttgatatga ttataagtga aggtattcca tatgttcttg
     3961 aactaaatac tcttcctgga atgactcaaa ctagtttaat tcctagaagt gctgctgcta
     4021 gaggtattaa gtacagtgaa ctattagata aacttataga atactcttta aattagatga
     4081 aataatttga ggtggtaatt tgatattttc aaatctaaaa ctaaatgata atgaaccaat
     4141 ttatatacaa cttaaaaatt atataagtga tatgatatca aaaggactaa ttccagacaa
     4201 tagtaagctc ccttctacaa gggagcttag tcagctttta caagttagcc gaaattctgt
     4261 agttttaact tatgaagaat taaaatcaga aggattaatc tactctattt caggaaaagg
     4321 tacatttgtt aaatcaaaga ataagtcttc aaatactaca tggtcattaa actgggattg
     4381 tttagagaat acatactcta aaaaagccaa tgaattggac attataaaaa gtgaaattcc
     4441 ttggagctct gacctgattt cctttaaaag tatttctcca gatggagatt tattcgacat
     4501 ggaagaatta aaaaaatcct ttttaaatag aatttcacta gaaggacata aattactcaa
     4561 ctatggatat gcacaaggtt ataagcccct tattgactat ttactagaat atatgacaaa
     4621 caagggtgct gatataagta ataaagatat actaataaca aatggattca cagaaggctt
     4681 tgatattatt atgtcatctt ttacccaaga aaaagactac ataatatgtg aaaatcctac
     4741 tcataatact gctataaaaa ttatgaaatc tcataacctc aatatcttag gtgtagatat
     4801 aaatgaagat ggtttggact tcaatatgct agaagacaat ttagttaagt ataaaaataa
     4861 aataaagttt gcatacatta caccctctta tcacaatcct acaggaattg taatgactcc
     4921 tgaaaatagg tacaaattct ataatcttat gaaaaaatac aatatagcaa ttatagaaga
     4981 tggatttaat gaagaattac tctacaatag ttctcatata tttcctatct gttccctaga
     5041 taatatgaat aatggggtcg tatatattgg tagtttttca aaaattttat ttcctggaat
     5101 gagaattgga tggatatttg cagataaaaa agtaatcaat aaactagaaa gtgtaaaaag
     5161 gtgcagaaac atacatgtat cctttttaga ccaaggaatt ttatttgatt atttaagtaa
     5221 tggaggtttt gaaaaataca ttaaaaaaat acgtaaattc tatggggaca aatttaactt
     5281 tgcatataaa tgtataaaaa aatatataaa aacagaatat atcttgggtg atggaggttt
     5341 gcatatattt ataaaactaa aaaatataga tactagagtt ttattagaaa aatgttatga
     5401 aaaaaatgtt atatttatgc ctggagattt attttttaca gataactctg gttttgatac
     5461 tttaagactc ggattttcta ggttgtcttt tgaagacatt gaaattggaa taaaaataat
     5521 tggagaaact ataaatgaaa tgttctaggt taactaaata ttgttagcct agtttatttt
     5581 tatgaggtat aatatatata gtttatatga atctaatact gggggataat atgagtaaaa
     5641 agtcaaaacg aagaaaaatt aataaattaa aattaacgat tgtaataact gtattatttc
     5701 ttgctttatt atctatatat gagatactgt tctctaatcc caacaaaaaa ggtaatataa
     5761 agagtcaaga aactatgcag aataaaaata atacaacaga tgattctaaa aataatcaaa
     5821 gctctaccaa aaagcaggaa gataataata tcaagcctac tttttcatat tcttcaatac
     5881 cagataatat aaaaaataaa atgattggta aatctatgcc tactgatgaa cctataagct
     5941 ttgatagtct atcttattta aaacttacat attatggatt tgatgaaaaa actcatcaag
     6001 gagaaatgat agtaaacagt gaactagctc cagaagtagt ggatattttt aaggaattat
     6061 acgaaaaaaa atatccaatt gaaaaaatta aattaataga tgactatgat gctgtagatg
     6121 aaaaatctat gtctgataat aatacctcat cattttgtta tagaactata gctggtacaa
     6181 atgtagtttc aaatcatggt aaaggacgtg ctatagatat aaacccatta cagaatcctc
     6241 aagtttctgg aaatgatgtt acacctaaaa taagtacagt ttatgctgat aggtctagca
     6301 caaaatttgg aatgataaaa aaaggtgatg attgttataa tgcttttgta agcagaggtt
     6361 ggagttgggg tggctattgg aaaaacccag attatcaaca ttttgaaaaa taatagatta
     6421 gaaataaagg tgaattatat gaatttaatg acattggaaa atataagtaa aagctattct
     6481 gaaaaaaaat tactagaaaa tatatctctt ggaataaatg agggggaaaa aataggactt
     6541 attggtgtaa atggtactgg taaatctacc ttactcaaaa ttattgcagg agttgaagaa
     6601 agtgagactg gtaatataac aaaaactaat ggtattagga tagaatatct acctcaaaat
     6661 ccaacttaca atgaaaattc aactgtactt gaacaagtat ttaatggaac ttctgaagaa
     6721 ttaaagctac ttggtgaata tcaagaaatc ctagaaaaac ttaattcaaa ctttacagag
     6781 gaattaaata aaaaattatt atcagtacac gaaaaaatag atgccttaaa tctttgggat
     6841 ttagaaagtg aagctaagtc agtacttaca aagcttggta taaatgactt taatcaaaaa
     6901 atagcagaac tttctggtgg gcaaagaaag agagtctctc tggcatcagc ccttattaca
     6961 ccttgtgagt ttttgatact agatgagcct acaaaccatc tagacaatga tactatagat
     7021 tggcttgagg aatatcttaa ttcaagaaaa ggctcattgc ttatgataac tcatgataga
     7081 tactttttag accgtgttac aaatagaatt attgaactgg acaagggtag attattcagt
     7141 tatgatggga attattcaat atttttggaa aagaaaatgg aaagactttc gctagaagca
     7201 agtattgaag ataaaagaca aaatttaata agaacagagc ttgcgtgggt aaaacgagga
     7261 gctcaggctc gtagcactaa acaaaaagct cgtttacaaa gatttgatga acttatgaat
     7321 agagattctt ttaaacctga tgaaaatctc gatatatctg ttgcatcaag tagacttgga
     7381 aataaaataa tagaaataca caatatatct aaatcatttg aagaaaatac tgttatagat
     7441 aatttagaat acactttaac tcgtacagat agaattggta taataggcaa aaatggtatg
     7501 ggtaaatcta cccttataaa tatacttaat ggagaaatag agcctgatag tggatatatt
     7561 tcaattggag aaacagttaa aataggttgt ttttctcaag acgactctca tatgaatatt
     7621 gatatgaggg ctatagacta tgtaaaagaa gctagtgact atatagaaac ttcagatggt
     7681 actaggatta cagcttctca aatgtgtgaa agattccttt ttaatggtac tatgcaatat
     7741 actttgatag gtaaattatc tggtggtgaa agaagaagac tacatcttct taaaatcctt
     7801 atgacagctc ctaatgtgct tcttttagat gaacctacaa atgatttaga tattgaaacg
     7861 cttaaaatat tagaagaata cctagacgaa tttaaaggtg tagtagttac agtatcacat
     7921 gatagatatt tcttagatag aatttgtaat aaaatatttg catatgaagg taatggaaaa
     7981 attcatatct atacaggtaa ttacagtgat tatcttattt atagagagat tcaaggaatt
     8041 gaatttgaag aatctaaaag ggaatcagtt aaaaaagatg actctactac cctaaaaaaa
     8101 gaaaaaccta aaaatgataa aaagcttaag ttctcatata atgaacaaag ggagtttgaa
     8161 aatattgact ctgatataga aaaactcgaa tctaaaattg caacacttga tgaaagtact
     8221 gcaaaatttt caactgattt tactaaactt caagaaattt tagatgagaa atctaaacta
     8281 gaaaaggaac ttgaatacaa atatgaaaga tgggaatatt taaataatct agctgatgaa
     8341 atagctaata ataagtgata ttaaaataat cttaagttta ataagttttg ctttaaatct
     8401 atactttata aatattttaa aggtagtgtc tcaaaatgaa ctttttagtt ttatgagaca
     8461 ctctttttta tgagaaatca aatatgtttt catcatttca acaatacaaa agaggcttat
     8521 ctctattttg agacagcctc tttcataatt accttctaat ttatatgctt taatatatct
     8581 ataataattc ttattatatc tatatatcca gtctttcaat atagtgacaa ttaacttcaa
     8641 tatgatttaa aaatactaac aacctttcat aaagtggatg agattcttct ccatatttag
     8701 cttctaggtt cttgcctatt tctctaacag tcctttgacc atcaatttgt aaaaaaacaa
     8761 aactggaata ttcatctagt tctatttttt tgtatttagg tattctagct tttaactttc
     8821 taaaaaactt ttgaatttta tggtcctgtt tttcaagtat agtaacaaca ctattttcag
     8881 ctacttcata ttcaagtcca tcagtaattt taaatacaat atctaaaaca tcattactct
     8941 tgctcattat tttttacttt agataaaaca ataggtaatg ttgtaagcac aactaaaact
     9001 actaataaag caattgccat tgaatttgtt gcagcaaatc cacttggtac ctttggagtt
     9061 acaactccag ttacttgtaa tattatacct attaaaccaa tgatagaacc accagcaaca
     9121 agaccagaag ataaacttat accattagaa actttaactt ctttttcttt ttcagtttta
     9181 gatactaatt caacaaataa acgaattaat gctccaacta atattataga agttgttgca
     9241 attggtaaat agaatcctat agctatagtc attattggta aatttaatgc atatagaact
     9301 attgccataa atactccaac aataatcata acccaaggta aactacctga cataatacca
     9361 gaagttaatg ttgacattaa gtttgcttgt ggtaatgcaa attgtacatt atcaccagtt
     9421 gctctaagtt ggtcagaaag taataaaatt actcctacaa caactataac accaactata
     9481 cttgcaattg taaagtaacg ttgcatttcg tttttgctac caccaataat ataagttact
     9541 ttttgagatt gagtataacc tccagctata gcaatagcaa caactataaa tgaaccaaat
     9601 aaaagtaatg atttattagc ttctaaatca gtccatccca ttataacaaa cactaatgtt
     9661 acaataacta gagaagctat agtcatacca gaaactggaa gatttgatgt tccaattgtt
     9721 cctgttaaac gaccagctac tatgacaaat aataaagata ataaaagaga tatgatagct
     9781 ccaataattg ccataacaat gttaccagaa attaagaatg ctgctaaaaa tccaataact
     9841 actccaccta ataaaagtat catttgtata gatgaacctt cttctccttc tccagcactt
     9901 gatttagctt taagtgtttc ttttatagaa gcgattatag ttggtataag ttttattgca
     9961 ccaataatac ctccacaaag catcatacca gcacctatat attttacata actactagat
    10021 attgctccga catccatttg gttaagaggc attgcaggat tattccatac catagctcca
    10081 tctttagcca tatctgtaaa gtatcctatt aaaggtgcta taccaaagtt tgataaaata
    10141 cttcctgcaa acattgttaa agaaacttca agccctacta taaatccaat tcctaaaagt
    10201 agaggattta cttcaatatc aaatttccat ttgtagaatt tgcttcctac aagactcatt
    10261 acattgtttg ctacatttaa gaatgaacca gttaatacag taataaatcc acttattaca
    10321 aatccaattc ccatgtactt aattgaatct ccaccagcct ctgaagcaac aagtgtctca
    10381 gatatagcca ttgattctgg atacataagt ttaccatgtt cttcaactat tagatagttg
    10441 tgaactatag cagcaattcc tataccaaat aaaactccac caactccaac agctaggcct
    10501 tcaaagaatg taatttggct tccaattaat ataactgctg gtaatacgaa aataaatcca
    10561 cttgcaacag attctccacc acttgacatc ccttggatta agtttttacc aagaataccc
    10621 tttgaacgag caaatgctcc aacaaaagca gaacctatta tagctcctgg aatacctgca
    10681 gcaactgtaa gaccggcttt cattccagag taagttgtag atgcggcaaa aatggcagct
    10741 aaaataatac cgataatcag tacagctaca tttccaccgg ttctggactt atctgtgata
    10801 tatggaacgt aatcttttcc actaacctca ccataagcac ccttaggtaa ttttttgttc
    10861 ataaaatttt cacccctttt caatttgtat atattataac atatatacat ataaaactta
    10921 actttttatt attttacaaa tagcgagtca aaactactaa aatttaaccc tatattatta
    10981 atttctactt ttagatattt ttatgctaat tttcttttta tttttcaatt ctactaaaaa
    11041 ctattaagat ataaaataaa aaaaattttt aatgtaaaat taattttgta aaaaaaatgc
    11101 tttaaaacac ggaaaagtat aaaaaataca ataatcattc ttataaataa attttcacca
    11161 tagtaaatat atacagaaaa ttcttttttt tacatcaatt gatttcctca gaaaaatagg
    11221 tgtcataaag aaatgtatgg aatcacttta caacacctgt cataataaaa aatcactctg
    11281 gtagtgcttt tttatttact tttcctattg gtgtaactgg tattttatct atcctcatta
    11341 ttattgttgg aatcatatat ggaggtagat gctcatttaa atattctcta atggttcttt
    11401 catttatctt accactatct tcctcatcaa ttacataata tgcacatata tacatatccc
    11461 cgtcatcatt tgttctcgat atacaaacaa cacttttaat ttctgtaata ttatttatta
    11521 tattttcaat ctcatatagc tctatcctaa actcatcaat ttttacttga ttgtccatcc
    11581 ttccaatata atctaaattt ccatcatcta accatcttac taaatcacca gttctataca
    11641 ttaacttatt atattcacaa gttttgtaag gattctctac aaatacttgc tctgttagcc
    11701 cttttttatt taaatatcct cttgatattt gaatgcctga tagacatagc tctcctggtg
    11761 ctccaattgg acatagtttg ttctttttat ctaatacata tgcataagta ttaaatatag
    11821 gttttcctat tggtattttt ccatattctc tgtccacatg aaaagaagtc gatacaacag
    11881 taaattcggt tggtccatac tcattatata gttcataatt acgctttcta tatcctttta
    11941 atctttctcc tccagtctgt acaactctaa gtgaattatt attgcaattt tgtataaata
    12001 accttgcaaa tgctgttggc aatgctagta tagttatgtc attttcctca cagtattcat
    12061 ttactgtata ttcatcaaat ttagcttctt caggcataat atctattgat gcacctacaa
    12121 gcaaataagg gaatagttgt ttcattacag catcaaaact gaaacttaag tatataccac
    12181 atatatcatt ttctgtagcg cttatagatt ttacgctata ataacacata tttactaaac
    12241 tcttatgttc tatagctatg gtctttggcc taccagttga ccctgaagta gatatcgtat
    12301 atgcacaatt tgatgatgtt attttttcag ttaattcata tccaagctta aacatttctt
    12361 catcatttat atcgagtact atggtattct catttacaac cttacttttg agttcatctg
    12421 tagttatcac tacttttgca ttactatctt caagaaggta attcaatctt tcttctggat
    12481 tactcgtgtc tattggaaaa aatgctcccc ctgatttcat aatccctatt gctgtaataa
    12541 ttatgttaat atctcttggt aacattacag ctactatgtc ttcactttcc actcctatat
    12601 gatttaaata acctgcaagt ttatcagtta cctcatccaa ttctttatat gttaactttc
    12661 tattttcatc cctagcagct atattactag gtgttacctt aacttgctcc ctaaataaat
    12721 caacataact tttatcatag ggtacttcat attgattatt attaaagtct tccaataata
    12781 ctttttcttc ttccttacat acaaattcta aatcttctat taaaatatca ttatcttcta
    12841 ttatttgctc tattatataa tcaaattgat ttaaaattct tctaatagtt tctcttttat
    12901 atatagatgg tttatacttt atttttattt taagtgccct cttagtatat actattacag
    12961 ataaatcata tccttttttt tctatgtcat aatcatatga taaatcaaaa cttttagcta
    13021 taaatattgt gtttataagc ttagtattta gatgattttt attctctata tctgataaca
    13081 aatagtatcc atactctaaa gacctgctcc actgcctttt tagcttcctt acaacatcta
    13141 taaatttttc actttgtcta actcttactc ttattggaat tatattcatt gttttaccaa
    13201 aaactacatc atcttcatat atatactttt gaagtataag gccatatata gtttctatga
    13261 tagagtctat gctaatattg ttcactttag aatattgaat taacctttca tgtttctcaa
    13321 tattcatctt aaaaacctca tctactactt catcagaaca atctttataa aaaaaatcat
    13381 ttggaatttt tgctatattc gtataatcat ctaacaaatc catccaataa ttcatagctc
    13441 gttctaaatt aacttcatac atatcaaaat tcccccttac tactaagtct atagaaaaaa
    13501 ttcttttgga attttatttt cacaatatat aaatatatta ataatacata tgcaagctct
    13561 aaaaacatga gcttgcataa aattacactt attatgacta ctaaattcgt aatatttaca
    13621 atgtttaaaa tatattataa cacatatatg attttatttt tctacattta tataatatat
    13681 aaattacaaa tagttattta actaatatat aacaatacct tttggaatat gatatattat
    13741 attaattaaa tttttataag ataagttata cttataggag ggaaccatat aaatgaatat
    13801 ttatgctcat agggggttta gtggaaagta tccagaaaat actattctag cttttaaaaa
    13861 atgtttgtat atggatatat atggaataga acttgatgtc cacagaacta aggatggaaa
    13921 aattgtagtt attcatgatg aaaaggttga tagaactttt aatggacatg gttttgtaaa
    13981 agactttact ttaagaaaat taaaaactct aaactcatct tttgaaggat atcaaaacaa
    14041 taaagaatgt aaaattccaa ctttagaaga agtattaatt ctaatttcac ctacagacct
    14101 tattttaaat atagaactaa aaactgacaa aataaactat cccaatatag aaaaagacgt
    14161 tttagagctt attttaaagt ataatatgaa aaatagagtt cttatatcta gtttcaacag
    14221 caatagctta aaaaactttc ataaattaga ccctagtgta aaaacaggat tattgtgtta
    14281 tttacctata aacaatgtag tcaattttgc taaatttctg ggtaatagtt accttcatcc
    14341 acctctagtt ttagtaaatg aatctctaat tgaactttgt cacaaaaatc tacttggtgt
    14401 caatgtatat actgtaaatg aagaggatga tatactacac tgtctaaaac tcaatgtaga
    14461 tggtattttt actaactatc cagatattgc ttcaaatctt ttacactcaa aacaatacag
    14521 ctaattatac aatagtttgt actccttgac ttttatataa ttaatatgta caattttatt
    14581 aaatttatct tggaggtata actatggata acttattaaa tgaacttgaa gctttaaaga
    14641 gtaatttaga taatctacca atagaaaaaa tagaaaatta tgatttgagt aaaactgctt
    14701 tatttataat tgatgttaat aatggctttg caagacaagg tgcattatac tctcctagag
    14761 tagagtcttt aataaaacca attgaaatgt ttacaaaaaa aatatcaaac aaattaaata
    14821 gagtaatagc atttactgat tctcatactc ctaaatcaat agaacttttg agttatcctg
    14881 ttcactgttt ggaaaatgat gtagaaagtg aattggttga tgaacttaag tcgatagaaa
    14941 acctacaaat tcttcctaaa aattctacta atggattttt tgcattagaa aatttggatt
    15001 ttgataatat agataatata attatagttg gcgattgcac agatatatgc atatatcaat
    15061 ttgcaataac tctaaaatca tattttaatc aacataatat tgaaaaaaat atagttgtac
    15121 ctatgaattt agtagatact tatgatattc ctaacattca ccctgctgag attttaaact
    15181 tagtattttt caacagtatg attcaaaatg gagttaatgt attaaaagag attcgttaag
    15241 cctctaatac tttacatccc atataataca attatcagat atttattttt aataaaattt
    15301 ctaatagaaa aacacagttt tagtgagaag attacaattt aaaacactat ctattatgac
    15361 taatcttcac aatataaaaa atcgcagttt agatattcct tatacaaatt tataaggaat
    15421 aataaattgc gatttttatt tttaagtagt acaattaatt gatactactt cagtatttaa
    15481 attcattttt taataatcat acttattcgt agtttcgcac attactacct ttattttttt
    15541 attaaacata ttcttatatt ctaataaaaa ctcatttagt tctgaatcca taagatttat
    15601 ttttctatca ctacaaagca ttatcttttc ttggtcgatt attacattaa tatcatttaa
    15661 gtcatttttt ataaatgttg ttatacaact ctttggttgg attttttctc catatttctc
    15721 ataaaatcct gcactcttct taaatgactc taaatcttct gtagatactt tgctggaagt
    15781 attattactc ataaaacctg ctgtagaagg gtcgattcca aagtttctaa gttgtttttc
    15841 tacttcttca gtgtccatac catatctctc cataaattgc ttttgtattt tcataaattt
    15901 ttcttgagat atgttgttat ctctcatata atcgaatatt tttcttgaaa actcagccat
    15961 ggacatcttt ccatccataa cagaaaaata taagttatat atgtattgct caaatttatc
    16021 tacatcagaa tctttaactt tgttctttag ttcgttaaag tctattattc tatcatcaga
    16081 catttttcat cctcctagct aaatttttac ttgtataaga ttataccata tttctattat
    16141 atttatatac acactatttt tttaatttaa cgattttttc tgtagcatct aattcgcaca
    16201 ttctgcccaa aggaatagtt atcattggga tacaatgtcc tgactgaaga ttatatatag
    16261 aaggtttatt gaccttattt gcaaattcta ttagtaattc catcaaatca ttttcttcaa
    16321 tacaatcctt aaaatctcca aaaattatac cattacattc ctcaagtttt cctgaagaat
    16381 atagttgtgt taacattcta tccagtttat attgaggttc tccaatttcc tcaataaaca
    16441 aaattttatc ttttgcatct atttcatatt ttgttcccat tgtggcaatt attaaggata
    16501 aatttcctcc cattattatt ccctctgctt taccttcaca tacagtataa atcttttctt
    16561 cttttggatt ttctaaatat aagtcatctt taaattctaa agcatttacc aatgatttat
    16621 aggtaaattt atcccattta cctacatttg ctgccattat tccatgatat ggtaaaagtc
    16681 cacaattttt ataaaaagct aaatttaaac ctgttatatc agaaaaacct ataaatggtt
    16741 ttggattttg ctttattata tcataattta ttttatccaa tattcttgtt gttccataac
    16801 ctcctcttaa gcacataatt atatctacat ctttatctaa aaacatatct tctatatccc
    16861 ttgctctaat atcatcatta ccagctaaat aacctttata atttaaacta caactttcag
    16921 aaaacttaac ttcgtaacca taactttcta aattaaactt tatttcctcc aaacttgtat
    16981 ttcttaatgg cccagatggc gcaattattc ctaacattta aacacctctt cttataaaat
    17041 gatatattct tctccatact aaatatctct aatataatta taagatattt tatttgtact
    17101 tgatacataa taatgattta cataaaataa tataattaat gaa
//
LOCUS       DAEPOZ010000062        17023 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.062, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000062 DAEPOZ010000000
VERSION     DAEPOZ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17023
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.062"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     repeat_region   10..566
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=10..38
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
     gene            756..1184
                     /locus_tag="KR440_001578"
     CDS             756..1184
                     /locus_tag="KR440_001578"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBH0698633.1"
                     /translation="MIKKLNNKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKSFIGALFIEPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
     gene            complement(1640..2419)
                     /locus_tag="KR440_001579"
     CDS             complement(1640..2419)
                     /locus_tag="KR440_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698634.1"
                     /translation="MKVNENNINNIDEELDELSRKVYLESPDFWISISDKIIDKVFDE
                     MLLFFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYL
                     LSLNKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDIT
                     YKFSSKEAEPIEMSSEVSSIRCCNCDELLEDITLNKLYNLCKIQNCNNCGSNLTKVGI
                     IAKKKMNFDENTEKFKDVSTSYCCGNCDSEINLSQREHFKI"
     gene            2743..3288
                     /locus_tag="KR440_001580"
     CDS             2743..3288
                     /locus_tag="KR440_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBH0698635.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            3320..4456
                     /locus_tag="KR440_001581"
     CDS             3320..4456
                     /locus_tag="KR440_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HBH0698636.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CNLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIERADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     PILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKTKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIERYNIFGGALAYGH
                     PYGASGAIITLHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            4460..5851
                     /locus_tag="KR440_001582"
     CDS             4460..5851
                     /locus_tag="KR440_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HBH0698637.1"
                     /translation="MNYYELLRIKKERHKNKNFLIIDGEKYTYENILSYSEQLGKQIS
                     LGENISILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNNEVAKKVNFSIFNPKFNLYMYQYKNLINYLDKSICMGALSSGSTS
                     VPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVI
                     SSGLNCKSWIRTIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTGSQLLFEYTAR
                     NLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYIRGGKIFVNT
                     EYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDIVNIGGFKVSSAKIENEVKKIPQIE
                     DAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTS
                     SEKIDRLALLKML"
     gene            5927..6802
                     /gene="dpsA"
                     /locus_tag="KR440_001583"
     CDS             5927..6802
                     /gene="dpsA"
                     /locus_tag="KR440_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HBH0698638.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEVGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            6805..7395
                     /locus_tag="KR440_001584"
     CDS             6805..7395
                     /locus_tag="KR440_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HBH0698639.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(7603..7803)
                     /locus_tag="KR440_001585"
     CDS             complement(7603..7803)
                     /locus_tag="KR440_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698640.1"
                     /translation="MKKLKTISIFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
     gene            8307..10961
                     /gene="adhE"
                     /locus_tag="KR440_001586"
                     /gene_synonym="adhC"
     CDS             8307..10961
                     /gene="adhE"
                     /locus_tag="KR440_001586"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBH0698641.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYTQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDESADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDNAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRSPGKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKHTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEEPKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
     gene            11237..13456
                     /locus_tag="KR440_001587"
     CDS             11237..13456
                     /locus_tag="KR440_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0698642.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYALNQDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKITISVPLLNNDK
                     VVGVLYCTYNINTLMKLINVSFYENNSISYVVRNDGTIILHPQEDSLSKNIYKLLKQD
                     NDIREINRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSEQIINRAIYAVLFIILIFIAIIFYIIYIKKSNEKEILSLAYEDKVTHIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGKNKRIRKIDSIRNNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LSEWKKQGKKIVPVSINLSRVELYQPDIVKFINKTIQMYNLSSDLIEIEITETVAINE
                     LNILKNVLNELRKYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLNGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            13710..14372
                     /locus_tag="KR440_001588"
     CDS             13710..14372
                     /locus_tag="KR440_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698643.1"
                     /translation="MIDVSIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            14742..16151
                     /locus_tag="KR440_001589"
     CDS             14742..16151
                     /locus_tag="KR440_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBH0698644.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLTSSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     SLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGGIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            16266..16541
                     /locus_tag="KR440_001590"
     CDS             16266..16541
                     /locus_tag="KR440_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HBH0698645.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DITVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
BASE COUNT         6712 a         1827 c         2777 g         5707 t
ORIGIN      
        1 aatataaaag ttttatatta actaagtggt atgtaaatca ttgtatacta agcttgatat
       61 agtgcaaaat gaaacgtttt atattaacta agtggtatgt aaatactaat tgggttaaac
      121 aaatggatat aactaaatta gttttatatt aactaagtgg tatgtaaatc caacccttcg
      181 cacgaatccg tgcgacgaaa tataaagttt tatattaact aagtggtatg taaattgtaa
      241 aaatattgct tagcatattg atatcactaa ctgttttata ttaactaagt ggtatgtaaa
      301 tacaagaggt ttttcagcaa tatatgtact tctataatgt tttatattaa ctaagtggta
      361 tgtaaattca tcaatattaa ttttgggaat atcaacattt tttgcgtttt atattaacta
      421 agtggtatgt aaatgcgata agagattcga gtctttttac tgggcttaat cgttttatat
      481 taactaagtg gtatgtaaat aggataaaga aaagactcac acaagacaca gtgtcaggtt
      541 ttatatcaac taagtggtag ataagtagaa tattagcaat atcaacgagt atttagaaac
      601 ttattttata ttaataaagc agtatataaa attgtagaat aaacaatagc atatactaaa
      661 acatccacct tatattaact aaatcatatc taaataaaat gcacttacct ataaacatta
      721 taaaatcaat acaaaaatga ggtgaaacaa aatttatgat aaagaaatta aacaataaag
      781 acataaataa aatcatggaa atatgggaaa aaagtacaat caaagcacat gactttataa
      841 gtaaagaata ctggcaaaat aactacaata ctgttaaaaa cgaatatata cctatatcag
      901 atacatttgt atatgatgat ggagatgaaa taaaaggatt tataagcata atagataaaa
      961 gctttatagg agctttattt atagaaccca aataccaaaa tctaggtatc ggaggtaaac
     1021 ttttagatta tgcaactaaa aaatataaaa gtctaagttt agcagtatat aaagataata
     1081 aaaaagcagt tgtgttttat aataaaaaag gttttaatat agtaaaagaa caagtaaatg
     1141 aagattcagg atttaaagag tacataatgg aatatagtaa ataatatgat tacattctat
     1201 taaaagaata attaatttat tttctatttg atttttataa gagtaatttt taaaatagac
     1261 tatatgaaga taaagatatt tattatagat gtaagttaat cagaattgat atagctacaa
     1321 aagaggaaaa taaaattgta tggtgtattt cagatttaac aaatttacta tttaaagata
     1381 ataagtttga tataatatta aagatacttt cacctataaa ctatagggta tttgaaagaa
     1441 ttttgaataa ttatggaatg ctaataaagg tttgttaaaa tttaataatt attagattat
     1501 agaaaattgc catgaaatta gttcagcaag aactagtttc atagcaattt taattcttat
     1561 aagtgtattt atttttacag atttaaaaaa cttatacagt atctaataat gtaatcttat
     1621 tactttagcc tataaatatt taaatcttaa aatgttctct ttgagataag tttatttcag
     1681 agtcacaatt tccacaacag taagatgttg agacatcttt aaatttttca gtattctcat
     1741 caaagttcat ttttttcttt gcaataattc ctaccttagt taaattagaa ccacaattat
     1801 tacagttttg aattttacat aggttatata gtttattaag tgtaatatct tctaataatt
     1861 catcacaatt acaacatctg atagaagaaa cttcactaga catttcaata ggttcagctt
     1921 ctttagaaga aaatttatat gttatgtcat ttgatacttt ataacctgta tcaaatatat
     1981 tagaattaca acttggacat aagtactttg gaagatatat atcttttttt tcaaaagtat
     2041 cactactaat attacagttt aattcatcta aaattggtct ttcatctagg ttattacctt
     2101 tatttaagct tagaagataa ttataaattt ttttatcttc attctgtttt gatgtatcta
     2161 acaaatgctc cattatatta gtgtattttt tgttttttga aggcatgttt aaatccagat
     2221 aattgttttc aatagcaaag catactatat tgaaatagtt atatttaact gcaaaaaata
     2281 ataacatttc atcaaatact ttatctatta ttttgtcaga aatacttatc caaaaatctg
     2341 gtgactctaa gtaaactttc ctactaagtt catctaactc ttcatctata ttatttatat
     2401 tattttcatt aactttcata tcatcacttc cttttaaata tcttgtgtat aaactatttg
     2461 ttactatttt ttatataaaa taaaatctta tataaaaata tatcatatta taatttacaa
     2521 gtatagtcta aatagagctg tttttaagca aagttttata aatgactatt aaaatataaa
     2581 gtaacagtat aaagtttact tataaattaa acgtaaacct aaatacttgt aatggttgac
     2641 aaaaaaacgt ctttatttta tactaaaaca aagctttaat agaggctttg tatatgtaca
     2701 tataaactaa atgaaataag acatatgaag ggaattataa aaatgaaaaa tactaaagta
     2761 aatattcaag atttaactaa aatgtcaata tgtgtagcat tattgtgtat atcatcttac
     2821 atatatatac cattgccatt tactccagca ggagtaacag cacaaacaat aatgataaat
     2881 ttgatagctt tgattttaac accaagacaa gcatttagta cagttggtgt atatataatg
     2941 ataggtttaa ttgggattcc agtatttggt ggaggtacat ctggtattgg aaagttgtta
     3001 agcccaacag gtggttttta ttttgggttt ttgtttgcag cactaattat tagtttatta
     3061 aaaggacgaa ataatgatat taagagatat atactaataa caatttttgt tggaatgcct
     3121 attatatatt ttatgggaac agtatttatg tgctatttta atcaaatgac agtagaagca
     3181 gcactttttg cagcagtagt accattttta attggagata caataaagtc ggttattgca
     3241 tcttttttag gcactaaact gaacaatgta ctcagaagaa atctatgata aatctataat
     3301 ataaggaagg gtgtaatgta tgaaggaagt ttttatatta ggaggattga gaagtcatat
     3361 aggtctaaaa aatggaattt ttcaatttgt tcaacctgaa ttactaggag caagtgtttt
     3421 aaaagattta attaaaaagt atgaaattga caaaattgat gagattatat gtggaaatgc
     3481 agttgggaca ggagggaata tagccagatt aatgacttta acagcaggag tgtcaaatga
     3541 agtacctgct tttactgtgg atatgcagtg tgcttctgct atgatgagta tagacatagc
     3601 tttttcaaag gttaaatctg gtcagtgtaa tttaattatt gcaggtggtt ttgaaagtag
     3661 ttctctacaa ccaatgcgca catatcataa gaatgataag cgatataata caaataatcc
     3721 aaattataca gttgctcaat tttctcctga tgataatagt caaaatagta tgctcgaagg
     3781 ggcggaaaga gtagcagagt tatatcaaat tgagagagca gacttggatt tttgggtaaa
     3841 ggaaagtcat aaaagagcaa aagaagcaag agaagaaaaa atacttgagg atattatatc
     3901 tcctataaat aatagtacct atgatgaagg tatacgtgat aagatgagtc aaaggttatt
     3961 agataggatg ccaccaattt taggaaaaga aactattaca aatgcagcaa attcatgtct
     4021 tattaatgat ggagcttcat ttattataat atgttctaag aagtatttag aacatgtaaa
     4081 aaagaagcct aaaacaaaaa ttattaatac atgcacaata ggaacagatg caaccttaag
     4141 tccaacatca gcgatacaag caatggataa acttttagag atagaaagcc ttaattattt
     4201 agatgtatct gctgttgaat ttaatgaagc atttgcagtt atagatgtgt tgtttcaaag
     4261 aaagtatcca gagttaatag agagatataa tatatttggt ggagctttag cgtatggaca
     4321 tccatatggt gcttcaggtg caattattac attacatctt ttaaaagcat tagaaaaaac
     4381 taagggtaga tatggaattt gctctatagc tgcagctggt ggtctaggtt cagctttact
     4441 gcttgaaagg gtgtagatta tgaactatta tgaactttta agaataaaaa aagagaggca
     4501 taaaaataaa aattttttaa ttatagatgg agaaaaatat acttatgaaa atatattaag
     4561 ttatagtgag caactaggaa aacaaatttc tttaggtgaa aatatctcaa ttttaatata
     4621 ttctaaaaat ataatgtttc agttgattag tttttttgct attaattatt caaaaaatat
     4681 tcctattatt tgtcattata atctttctaa aaaagtatta gatgatattt tattaaaaaa
     4741 taatatttca atcattatat ctgatgaatc tatggacgtt attgatttat atgatgataa
     4801 caataatgaa gtagcaaaaa aagttaattt ttctattttt aatcctaaat ttaatctata
     4861 tatgtatcaa tataagaacc ttataaatta tttagataaa agtatttgta tgggagcttt
     4921 atcttcaggt tctacaagtg ttccaaaggt attatataga acatatgaga gttgggctgg
     4981 attttttcct atacaaaatg gtatttttaa tatctctggg gaatcaatct tgtttgttaa
     5041 tggaacactt agttttacag ggaatctaaa ttctataatg tcagtattat atgaaggagg
     5101 tagtattgta ataagttcag gtcttaattg taaatcatgg ataaggacaa taaaacaata
     5161 taatgtaact aacatatacc ttatacctac aaaacttcaa cttttggtta aacacttaaa
     5221 agaaccagta ctcaaagtag taagtatttt tacagggtct caattattat ttgaatatac
     5281 tgcaagaaat ctaaaaaaat atatgccaaa gtctgaaatt attttatact atggggcaag
     5341 tgaattaaat tatattacat atttatgcta tgatgaacta attgaaaaac ctctaagtgt
     5401 aggcagacca tttccaggaa tagatatata tattagagga ggaaaaattt ttgtgaatac
     5461 agaatatgca gtatataatg caaccaaacc ttattctgtc aatgatattg gatattatga
     5521 taatgatgga tacttaatat ttgaaggaag aagtgatgac attgtaaata ttggaggatt
     5581 taaagttagt tctgctaaga ttgaaaatga agttaaaaaa attcctcaaa ttgaagatgc
     5641 aattgtattg ccgtactcag acactataag aggaaatcaa attgtattat ttgtaataac
     5701 tatagacaaa attacgaaga aagatttgtt gattaaaatg aaagataatc taatgaaaaa
     5761 tgaaatccca aagaaaatta tctttttaaa ttcatttcca tatacctctt ctgaaaagat
     5821 agatagatta gcattattaa aaatgttata agtataatat ttagtaattt tagtgaacca
     5881 atgtattgat aaaaacatat tgtatatagt aaaggaggct tacaatatgt caaatcaata
     5941 cgatataact gttattgggg gagacctgcg acaagtgtac atggtaaaaa aactaattaa
     6001 taaagggtat tctgtagtca cttatgggat atcaaatgaa attataaatg gaatcgttgg
     6061 aaaatcaact tcactagctg aagcactatc caaaagttcc attattttat gtccaattcc
     6121 atttacaaag aatcaagtta atattgaaaa tacggatgat tgtcttgatg ctactattga
     6181 taacttgcta attaatttat ctcctaatca aatattattt ggaggtaaca taccaactaa
     6241 agtttgtaaa ttttgcgagc aacactctat tcagatttat gattttatga aaatgaatga
     6301 tgttgctatt ttaaatgcag ttgctacagc tgaaggggct atagcagaag ctattaaaag
     6361 aagtataatc aatatacatc aaagtaactg tttagtatta ggttatggaa gatgtgcaca
     6421 aatacttgca aaaaaattat acgctttgga tgcaaatgta tgcgttggtg caagaaataa
     6481 tgatgcaaga agtacggcta atgcatttgg atattccagt atttctttaa gtcaacttga
     6541 tgaaaaactt ttacaatttg attatatttt taacaccata ccagagtgta ttttaacgaa
     6601 agaaagatta gaaaaagtat cacaagaagt aactattata gatatagctt cttctcctgg
     6661 aggagtagat tattctgttg ttaaagaagt agggattaat gcgactcttt gccttgggct
     6721 tcctggtaaa tattcaccaa agacatctgg tgagattctt gttaacgcta tagaaaatat
     6781 tataaatgaa agaagggatt agaaatgaga cttgaaggac ttacaattgg agtaggattt
     6841 actggttcgt tttgtactta tgataaaata tttatagagt tagagaattt ggttaaagaa
     6901 ggagctaatg tacacactat attttcagat gtatcacaaa atatcgattg tagatttgga
     6961 aattctgaag agtttatgaa aaaagcatat gaacttacag gaaataaacc tatagttacg
     7021 attgaagagg cagaaccatt tggacctaaa gggattgctg atattataat aatagctcct
     7081 tgtactggaa atactgctgc aaaattagca aatggtatta cagattcacc agtattaatg
     7141 gcagcaaaag gtcatcttcg taatgataaa ccactagtaa tatcaatatc aacgaatgat
     7201 gctctaagct ttaatttcaa aaatattggt attctcctta attctaaaaa tatatatttt
     7261 gtaccttttg gtcaagataa ttgcaaagca aagcctaatt ctatgattgc acatactgag
     7321 ctaataattc caactataga acttgctcta gagaacaaac agctacagcc tgttataaaa
     7381 agtccacatc aatagaattg gttcttataa tattatgtat tagatacaat actagcaaag
     7441 aggctgtctc aaaatagaga gaagcctctt tttcattgtt gaaatgatga aaatatattt
     7501 aatttcatac aaaaaaatgc atcatgaact aaaaagtcta ttttgagcta atctccgtaa
     7561 tttttggtat attaaaatta tttacttaat ccaattttta aattagaatt tatatcgttt
     7621 tgactcttca aatatcaatc taactgttgt tgatacaaaa gaagaggaca taattaaaac
     7681 aaatactgat agtcctctaa taaacaaatt atctacataa taagttacta ttccaatacc
     7741 tccaatcgtt aaaattacac ttataatcaa agaaaaaatg ctaattgttt ttaatttttt
     7801 catgcttatc ctccttctta taaacttcaa atttatctat ataatattaa atatatttta
     7861 tttcatagta tataaaaatc atgaatatat atttttttaa ttatactata tgttgtaaat
     7921 gtttgtacat ttaactatat ttactttaac ttgttaatca agttattatt ttcattttca
     7981 agtattttta tttttaaact tgatagaata tcatattaaa aattcttttt ataaagtttt
     8041 atttgcaaaa aatacaaaaa tataaacgtt aaaaaaataa cgttatcctt aagtttttag
     8101 atgattttat tagtgatttg cgtaaaaaat aaagtgatat ctagttaaat taagtatttg
     8161 taaagctaga tattattagt agaaatgatg ttatattaga taaaaaataa aatgttaaaa
     8221 aaataacttg aaaatagaaa aagtaaatgc tataatgtta ataaattaac ataataaata
     8281 aaaaactatc tcaggaggtt gttaaaatgg ctcataaaga acaagtaata atagaaaata
     8341 aaaaagaaaa tgaagttatg tcagttggtt tagatactgt aaataacgtt gaaagcttag
     8401 ttaaaaaact agcaaaaatc agagaagctc aaaaaatatt tgctacatat actcaagagc
     8461 aggtagataa aattttttta gcagcatctt tggcagctaa taagcaaaga gttcctctag
     8521 ctataatggc acaaaaggaa actggtatgg gaattgctga agataaggtt ataaagaatc
     8581 attatgcctc agagtatata tataatgcat ataaagaaac taaaacttgt ggtgttatag
     8641 agaaagacga agcatttggt atgactaaaa ttgctgagcc aataggtgtt attgcagcag
     8701 ttgtacctac aactaatcca acttctactg ctatctttaa agcactttta gctttaaaaa
     8761 ctagaaatgg aataattttc tctccgcatc caagagcaaa aaattctact atagaggctg
     8821 caaaagttgt tcttgaggct gctgttttag ctggagctcc tgaaggcata ataggatgga
     8881 tagatgagcc atctttagaa ttaactacaa ctgttatgaa agaagtggat ttgacccttg
     8941 ctacaggagg tcctggaatg gttaaatcag catattcatc tggaaaacca gctattggag
     9001 ttggtgcagg taatactcca gcaataattg atgagagtgc agatataaaa actgctgtaa
     9061 actcaattct tgtatctaag acttttgaca atggtatgat atgtgcttct gaacaatcgg
     9121 ttatagtatt agaaaatata tacaatgaag tcaaaaaaga atttaaagag cgtggtgctt
     9181 atttattaga taaagatgaa acagaaaaaa taagaaatat aattttagta aatggaggat
     9241 taaattctaa aatagttggg caaacagctt gtactatagc aaaacttgca ggctttgaag
     9301 tcccagtaga tactaaggtt ttaataggtg aagtagaatc agttgaaata gaagaagctt
     9361 ttgctcatga gaaactttca ccagtacttg caatgtacaa ggccagtgat tttgataatg
     9421 cagttagaaa agcagaaaaa ttagtagaag atggtggatt tgggcataca tcttcattat
     9481 atattgatga tgtaaatcaa agagaaaaac ttgataagtt tacttcagcc atgaaaactt
     9541 gtagaatttt aattaatact ccttcttctc aaggtggtat aggggactta tataatttta
     9601 aactagctcc ttctcttact ttaggatgtg ggtcttgggg tggaaattca gtatctgaaa
     9661 atgtaggagt taagcattta cttaatatta aaacagtagc tgagaggaga gaaaatatgc
     9721 tttggtttag atcaccagga aaagtttatt tcaaaaaagg ttgtttaggg gtagcattaa
     9781 aagaacttaa agatgtaatg aataagaaga gagcttttat tgtaacagat acattccttt
     9841 acaataatgg atatactaaa gcagttactg atttattaga tgaaatgaat attaagcaca
     9901 ctacattctt tgaagtagaa ccagacccaa ctttggaatg tgctaagata ggcgctaaag
     9961 ctatgagaga atttaatcca gatgtaataa tatctatagg tggaggaagt gcaatggatg
    10021 caggaaagat aatgtggaca ttgtatgagc atccagatgt agatttccaa gacctagcta
    10081 tgagatttat ggatataaga aaaagggtat atacattccc taaaatgggt gaaaaagcta
    10141 attttgtagc tataccaaca tcagcaggta ctggttcaga agtaacacca tttgctgtta
    10201 taactgacca agatacaggg gtaaaatatc cattagcaga ttatgaactg atgccaaata
    10261 tggctatagt tgattctgat atgatgatga atatgccaaa aagcttaaca tctgcttctg
    10321 gtatagatgc attaactcat gcattagaag catatgtatc tatgcttgca actgattata
    10381 caaatggatt agctttacaa gccattaaga gtatatttga atatcttcca cgtgcatatg
    10441 ataatggtgc aaaagaccca gaagctagag aaaaaatggc aaatgcttct acaatggcag
    10501 gtatggcttt tgcaaatgca tttttgggtg tttgtcactc tatggctcat aaattaggtg
    10561 ctttccacca tgtaccacat ggtgttgcaa atgctctttt gataacagaa gttatgaaat
    10621 ttaattcttc agatgcacca aagaaaatgg gagcattctc tcaatataaa tatccagaag
    10681 cattaaaaag atatgctgaa atagcttcat ttttaggatt aaagggaaat tctgatgaag
    10741 agaaattcca aagtttattg gtagctattg aagatttaaa acttaaagtt ggtattccta
    10801 aatctataaa agagtttgga gttgaagaac ctaagtttat ggattcaata gatgaaatgg
    10861 tgattcaagc atttgatgac caatgtacag gagcaaatcc aagatatcca cttatgaatg
    10921 aaataaaaga tatgtattta aattcatatt atggaagata aatttactat aaaatagata
    10981 aaataaaaac tcttatacat aaaacaaagt tttattgtat aagagttttt ttgtttagaa
    11041 aaattgatag tttttattct ttaatttaaa attcaactaa atagtgttat tatgataata
    11101 aaagaatgtt tttatggtta ttaggttgat tttatgtgta acatttaaga atagttatat
    11161 acaatataaa tgatgaatcg gtataaaaag gatatattat ctatatgaag tattgactaa
    11221 ttaggggagg agtcgaatga cagggagaaa taaaagtttt acattaatta caattctttt
    11281 tttaacatca ttatttttga caatggggta tatctatata gaagatacaa aacatcttct
    11341 tatgtcagag gcaaaaatac atataaaaga agttgcagta caaggctctc aactagcaca
    11401 aagacaaatt gaaaaagatt tagatgtttt aaatattttt tctaactatt atgctttaaa
    11461 tcaagatata cctaatgagg aaaaaatgaa aaatctttta gatgaaatgg aaaatcaaaa
    11521 attttacact atggcaatag ttgatataaa tggtaatgct gaaaatacca aaggtgataa
    11581 gttttcagtg aaagatagag aatttttcaa aaactcaatt aaaggtaaga agtatgtttc
    11641 aagtccatat gttgatgaag taaataagtc tataaagaag attactattt cagtaccatt
    11701 attaaataat gataaagtag ttggagtttt atattgtact tacaatatca atactctgat
    11761 gaaacttatt aatgtatctt tttatgaaaa taatagtata tcttatgttg taagaaatga
    11821 tggtacaatt atccttcatc cgcaagagga tagcctatca aaaaatattt ataaattgtt
    11881 aaaacaggat aatgatattc gagaaataaa tagattaaaa aaagaattgc aagaaaataa
    11941 aacaggtgca actgtattaa atatgttgga agaaagaaga tatttaggat atgctactat
    12001 ggataatggg aatagtgaaa ataattatat aaaagattgg aatttgattt tttcaattcc
    12061 agaaactgtt gtatttagta attcagaaca aataatcaac agagctatat atgcagtgtt
    12121 atttattata ttaatattta ttgcaattat tttttatata atttatataa aaaaatccaa
    12181 tgaaaaagaa atattaagtt tggcttatga agataaagtt acccatatag gaaatcaaaa
    12241 taaattttat agagagtgta gtaagtatct tttagataaa ccatctttaa attatattat
    12301 agtatatttt gatattaata actttaagat gattaatgat acttttggat atgaatttgg
    12361 tgacaattta ttaattacga tagcaaaagc tttaaaagaa gaattaacag aaggtgaagt
    12421 atatgcacga ctttcaagtg attattttgc aattttctgt gattataaaa atggaaaaaa
    12481 taaacgtata agaaaaattg atagtatacg aaataatatt gaaagtaatt tgagtatagt
    12541 atttgaaatt tctctgtgtg tgggtattta ttttgttgaa gaaggtgaag tagatatcca
    12601 aaaggctgta aataaagcaa atatggctag gtctgttgct aaaggtaaaa atataaatta
    12661 tgctatttat aatgaagatg ttaggaataa gcttagtgaa gaatcaatga ttttagatga
    12721 tataaaaata gcattggtaa aaaatcaatt tgaagtgtat tatcaaccta agttttcact
    12781 tgtaactgga gaaatgatag ggagtgaagc actaataaga tggaatcatc cagaacatgg
    12841 ttttataagt cccgcagtat ttattccaat agcagagaaa agtaagttga ttttaaaaat
    12901 tgggagattt gtatttgaaa gggtttgcaa tgatttatct gaatggaaaa agcaaggtaa
    12961 aaagatagtt ccagtttcaa taaatttatc tagagttgag ttgtatcaac cagatattgt
    13021 aaaatttatc aataaaacaa tacaaatgta taatctaagt tcagatttaa ttgaaataga
    13081 gataacagaa actgtggcta ttaatgagtt aaatatttta aagaatgttt taaatgagtt
    13141 gaggaagtat ggattttcta tttctatgga tgactttggt acaggatatt cttctattag
    13201 ttgtttaaga gatatgccaa ttgatatatt gaaactagat aagtcttttc ttaatggtat
    13261 tgaacatgat gagagaagtc gcaatatagc aaaatcaata gtgtctctag caaaatcact
    13321 agatttagtt gtaattatag aaggtgttga gagtaaagaa caagctgagt taatgaaaca
    13381 atttggatgt gatttagttc aaggatttta ttttgcacga ccaatgccag ctaagaattt
    13441 tttagattta ctttaaaaca aaatttaatt tttatataaa gcactattat gaatgtttga
    13501 ttttaataaa tcactgctaa ataattagtg tttttttatt ttatcaatgt attgtttaat
    13561 ataaagcaaa aaataattgt attttataaa aattatatta aaaataaatt tattttgcta
    13621 ataaacatgt tattaacaaa agaatagtac tataatgtat agatgataaa aactttaata
    13681 aaaatttagt gtataggagt tgtttaagta tgatagatgt gagtattgat gacaaactta
    13741 aggaaagatg gcctaatgct aagttgggtt gcattcaagc aaaagtagca gtaaataaaa
    13801 gctcagaaaa attaattaat gaaataaatg agttttgtga tactataaat caaagtctaa
    13861 aagttgaaga cattacgaaa ctagataaaa ttaaagatgc aagaaatgct tataaagagc
    13921 ttggaaaaaa tccaagtaga tatagaacat cttcagaagc attggttaga agaattattc
    13981 aagggaaggg tctttataca ataaataata tagtagaaat aaacaattta atatcaataa
    14041 aatcattata tccagtaggt acatataatg taagcaaatt acattcacca gtatgtttta
    14101 ctgtaggaga tgaaggagaa cagtataagg gtattgggaa agaattaata aatatagaaa
    14161 atcttcctat attgtctgat tcagtgggta aatttgggag tccaactagt gattctgaaa
    14221 gagcaatgat aacagaagat gcaaatgaaa tattaatttg tatattttct tttagtcaag
    14281 aatctgatat tgaagaatat tttggatatg ctaaggagtt acttaaagaa tatgctgatg
    14341 gaaaagatat agaaactaaa ataattaaat agaatctata taacaacgtt atgttaagct
    14401 aaaattcaat ataaaaataa gttaagataa agaaactaac ataaaattag tttctttttt
    14461 aaaagagtag tttagtgtta caaaaacaga tagataaccc acgattatta aaagtatggt
    14521 aatatattaa tatagcagag attgtattta agggattttg aaaggggtga tttataatag
    14581 tcattttgtc taaaagataa aatggtttta tggaaataac tatatattta aaaatataaa
    14641 caatgataca gtaatttggg tatataaaat aaggcataca tggttttgtt taaaaaccaa
    14701 taaagtacat aggtaattac aatgtaagga aggatttaat tatgattgat ggaaaagagg
    14761 taaaacaaga aagtggacaa aaaaatctaa aatttaagat agctatagtt gtaggtgttt
    14821 tgtttgtagc ttatatagga atggcaatct tttttagaaa tcatttttac tttggaacga
    14881 ctattaatgg cgtaaaggct tcaggaaaaa cagttgagca agtaaatgat ttgttaacat
    14941 ctagtactaa ttcctatact ttaaacttag aggaaagaaa caataagaaa gaacaaataa
    15001 aagcaaaaga tattgattta aaatttacac cagatgataa agttcaaaag ataaaagata
    15061 gtcaaagcgc tattggatgg attggtggaa tatttaaatc taaagagtat aataatatga
    15121 taactctttc atataataaa gatttactag aaaaaaggtt taatgcttta tcttgttttg
    15181 atagtaaaaa tgttgtagaa cctaagagtg cttatcctaa gtatgatagc actaaaaacg
    15241 cttatgttat agtaccagag gtacttggaa acaaagtgaa aaaagatgat ttttataagt
    15301 tagtacaaaa ctcaattaat tctgggaata cttctatttc tctggatgaa agtaaagttt
    15361 atgaagcacc aaacaacaca aaggattcag aaaagttgaa acaagctatt aagacattag
    15421 ataagtatgt tggtgtagtc gttacatatg attttagtga ccgtaaggaa actttagatg
    15481 gttctgaaat taataaatgg ttaaaagtag actcagaaaa gaattatgac ataacttttg
    15541 ataatgatgc tatggtagaa tatacaagaa gtttaagtaa aaaatacagt acttttggtg
    15601 actcaagacc atttaaaact gccacaggta aagatattac aatttcaggt ggaatttatg
    15661 gttggttgat agataaaaag aaagaagctg aagcattagt tgatgttgta aaagctggta
    15721 aaaatgttac aagagaacct atttacatac aaaaagctgt atctcgtaat aaagatgata
    15781 ttggaaatac atatgtagag attagtcttt ctggccaaca tatgtggttc tttaaaaatg
    15841 gagaaatgct tgtatcaaca gatgttgtaa caggcgattt agctagagga tttgctacac
    15901 catcaggtat atatcctctt aattataagg ctagaaatgt ttcattaact ggtcaaggtt
    15961 acagttcacc tgtaagttat tggcttccat ttaatgggaa cataggcatt catgatgcta
    16021 cttggagaaa ttcttttggt ggaggtatat ataagtcttc aggttcacat ggatgtgtaa
    16081 atacaccttt ttctaaggca aagaaaatat atgaaaatat agaaccaggt acaccaatta
    16141 ttttatacta gtaaatgtgt tgctaacagt taagcgaagt catcttagtt tatttaggat
    16201 ggcttccttt ttatataaag cttattgaag tttacagaaa gactacttaa aaggagaaat
    16261 acataatgaa tgaaaaatta actaaaattt tagaagaagc taagaatgat gatgaattta
    16321 gaaaggaact tataaaaaca agagaacaac gtgatgttat ggattcattt tgtaaggttg
    16381 ttacatctag aggttatgat ataacagtag gggaactttt ttccatggga gaagaatata
    16441 catcaaacct actgaaaagt gtaaatggag gagcagcata tcctattgaa gattggagtg
    16501 attggtacga ggattttttt ataatgttgt cgaatatata gagtaactaa tgatattatc
    16561 tacttaagat taatacattg ccttaaaata aaactattag aactaagtgt aagttttatc
    16621 aatctttaaa aatattgatt tatttctaac tatatataaa aaatttagat tgaaaataag
    16681 gtgacaaaaa tcgatatagc ttgatgattt ataaaagtta gataaaatag tgcgagatat
    16741 ttctaaaaat tcaaaaaaaa caataaactt caagggttag aggttgaaaa agaatataaa
    16801 aaacttttat atgtaataaa ttaaattaaa attataaaaa aaataaaata aaaatttaaa
    16861 atgtcgacat agcgttaatt ttaaaacaaa ataacgctat tgacaaacta tatcgcttta
    16921 aattataatt attgaaaata atggtattta aaaaccatac tcatgtcata gtctaaaagt
    16981 aagtcaaaaa aggatgtaaa tctaggcaag tactattcta aaa
//
LOCUS       DAEPOZ010000063        16951 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.063, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000063 DAEPOZ010000000
VERSION     DAEPOZ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16951
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.063"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..557)
                     /locus_tag="KR440_001591"
     CDS             complement(<1..557)
                     /locus_tag="KR440_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0698646.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNALIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNL"
     gene            1028..1678
                     /locus_tag="KR440_001592"
     CDS             1028..1678
                     /locus_tag="KR440_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HBH0698647.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            complement(1818..3194)
                     /locus_tag="KR440_001593"
     CDS             complement(1818..3194)
                     /locus_tag="KR440_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0698648.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(3210..5123)
                     /locus_tag="KR440_001594"
     CDS             complement(3210..5123)
                     /locus_tag="KR440_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBH0698649.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            5259..5891
                     /locus_tag="KR440_001595"
     CDS             5259..5891
                     /locus_tag="KR440_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBH0698650.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            6030..6569
                     /locus_tag="KR440_001596"
     CDS             6030..6569
                     /locus_tag="KR440_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBH0698651.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAARKYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FQMDREGYKKYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFNKYERFYEEY"
     gene            complement(6745..6915)
                     /locus_tag="KR440_001597"
     CDS             complement(6745..6915)
                     /locus_tag="KR440_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0698652.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
     gene            7200..8267
                     /locus_tag="KR440_001598"
     CDS             7200..8267
                     /locus_tag="KR440_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HBH0698653.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSIEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSSSRNNYQPKVL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            8437..10338
                     /gene="cooS"
                     /locus_tag="KR440_001599"
     CDS             8437..10338
                     /gene="cooS"
                     /locus_tag="KR440_001599"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBH0698654.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAEYIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            10331..10804
                     /locus_tag="KR440_001600"
     CDS             10331..10804
                     /locus_tag="KR440_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBH0698655.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSKHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            10779..12005
                     /locus_tag="KR440_001601"
     CDS             10779..12005
                     /locus_tag="KR440_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBH0698656.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDVCY
                     ADFFNIKENGEFCYSV"
     gene            complement(12144..13331)
                     /locus_tag="KR440_001602"
     CDS             complement(12144..13331)
                     /locus_tag="KR440_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH0698657.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRVVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKSFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            13604..14428
                     /locus_tag="KR440_001603"
     CDS             13604..14428
                     /locus_tag="KR440_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBH0698658.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GNNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            14557..15822
                     /gene="gluD"
                     /locus_tag="KR440_001604"
     CDS             14557..15822
                     /gene="gluD"
                     /locus_tag="KR440_001604"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HBH0698659.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            16015..16671
                     /locus_tag="KR440_001605"
     CDS             16015..16671
                     /locus_tag="KR440_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBH0698660.1"
                     /translation="MYFLPPHDYKLEELDWIFGHELMYFKSKDLHLKYLVLFLKIVYW
                     FNPFIYIMEKYMDLDCELYCDERVLKNCTVQQKQEYALTIVNSMKRGINFSNKFVTGL
                     NKQSDIRKRVMNMFSEKHKNGILMALIISLFSSITFFKFDSTSLIEENDFLERSRYAF
                     EGRQEDMFVTIDSTYADAPIKLRKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK
                     "
BASE COUNT         6451 a         2044 c         2727 g         5729 t
ORIGIN      
        1 agattagatt gtatttcctt tgaatcaatt tgtaataaat ttattatttc tataaatgta
       61 gaaatatcta tatatctgtg acctaaatca aatatatctg agataaagct aggtactttt
      121 tctggaactc ctggagttat agctattttt atatttttat agttattatc attcatatat
      181 ggaattggtc taatattagt cactccaatt ttataaaata aacttatagt ttctagagta
      241 gtttcatcag aatcatttac gattagagca tttgtaccct gtggtaaaga tagtagatta
      301 taaattttat cttccctaaa agttcttctt accacaatca cttttttctt atctagtata
      361 ttatttataa ttttgtctaa tctttctact gacattacta atatcacatc atcatttata
      421 atatcattat catttatttc atttatgaag tatctattaa tatttataga atctccaaaa
      481 actaatttca aattattttc taaaaagtct attaaatctt ttctactatc atttaccaga
      541 gctactgatt ttttcattta tttctcctta tataataaat agtttccaac aaaatagttg
      601 tataaagcaa tgtaattttg ataatttaaa tttatcaaat aaaaaagaaa gtgtctataa
      661 tgactttata aaagttgcat taagacactt tctttcttat aattaaaaat cttgtcacca
      721 aatcccccta attgttgctt aaaacttaaa ctgtatgtat atttttcatt tctattcgat
      781 taaattataa tatacaccca aaaaaattta ttttaataaa ttctattgat aatataattt
      841 tgtaaatata gaattatatt aaaataaatt atatatgaaa atatatatgt ataagtttat
      901 tctgcgtgag tagaatgaat ttgtcaagtt taatttagtt gtaattgtat cttttattaa
      961 aaataatata aattaaaaca tataaataaa atattttata caatatattg taaaggaggt
     1021 aactttgatg aaagcagaaa aaacaagtat atatccaatt attaaaaata atttaaacaa
     1081 aatatcaatt gttgaatata aaaagggtca aagatttgta gcatcagata aagacttaca
     1141 agaagtgttc tttataatag agggagttgc attagtggag tgtactacta gaggaggaaa
     1201 taaatttctt gttgacattg tacctgaaaa tgaatttata ggcaaaatca gttacatata
     1261 tgaacataat ttgaaatgtg atatatttgc aaagaccaat ataaaattat ttcgatttga
     1321 aaaaaatgta tttgaagagt tttatctcaa accagatttt attgcactat ttcacagaaa
     1381 atgtacaaga agaatatatg aattatataa aaccagaatg gtaagagaac tattttcttg
     1441 cacagaagtt attgcctatt gtatatcaaa taaacaagaa aataatgtct gcaatataaa
     1501 agctattcgt aatttcagtg aaatctattc aattagtaga aaaagtagat accattctct
     1561 gctaaagtta actgaaaagg agatagtaaa aaaaatgggt aatacatatg aaattttaaa
     1621 ctatgatgaa ttacataaat tggcatttga agtaaaagag tttttggaag atgagtaaaa
     1681 attgttactt aatcaaaata aagtataata atttctgtaa aaaatagaaa tagtctcact
     1741 gaaaatcaaa aatactttag ttattttcaa tgagactgtt tagaaatatt gtatacttat
     1801 aatgtatagt ataattatta atcaatttcc tttactatat taatagccca ttttttagat
     1861 aatgctctag gaacaccaaa tataaactta accacttctt cactataagt gagtaaaacg
     1921 agtacaaata taggtgcacc tttaaatgca gctataaaag ttagaggaat agaagctaac
     1981 cacatacatc ctaattctag aaatagagca tatttagtat ctcctccact tcttaaaata
     2041 ccaattataa ccaatgtatt aaaagattta agagccatta gtattcccat gataaagaat
     2101 atcttagtta tatcagaagc taagctatca gaaacactaa acatttttaa taataatggt
     2161 atatttaaaa ttaaaataat tccaagtatt aaaccagctg aaaatacaag tatagaaaac
     2221 tttttagcat attcaatggc tcttttaata tggtcagctc caagttcatt tccaagcata
     2281 atagatgcac cagatgcaat acatacagct gtcattatga agaaattacc tgtactggtt
     2341 gctatctggc tagctgctat agcagaagta ccagctcttg catatgcaac agaatacagg
     2401 acagttccaa ttgcccatac agagtcattt acaaatatag gaaagctttt tgatgaaaaa
     2461 gattttataa aaattttatc aatagctttt agatttttta gaccaaactt taatatatag
     2521 tcttttttat aaaggtaaac ataagtaatc attaaaatta attcacatat tctagcaata
     2581 acagtagcta gagcagcacc ttttacaccc aatgcgggaa gaccgaagtt accaaatata
     2641 aatccataat ttaaaataac atttgttaca agtgcaaatg cactacaaat catacctaac
     2701 ttaggatttc taacacctct tgaaccagta ctaaatacag tacttacagc aattaatgga
     2761 taactaaata ctgcaatact aaagtaatct atacaaagtt ttaccacctc agaatcatac
     2821 gaaaatatgt gtataattgg ctttggtgtt agtaaagcag gaataacaaa tacaaaactt
     2881 aaaacaattg ccaatacaca agtaagacct gtgacttttc ttatattact cacatccttc
     2941 tttccataaa actgtgaaat aaaaactcca gcacctcctg tgataccaga taaggacata
     3001 ttaaaaagaa agaaaaattg gtttgcaact ccaactgaag ctacagatgt ctcccccaaa
     3061 ctacttatca taattgtatc tattacattt acagaagtag aaattaagtt ctgtatgata
     3121 ataggtatac ataaagatag aagtatttta taaaatcttt tattttcgtc ttttaaagaa
     3181 aatagttctt gcaaataatc ctctcctttt tacataaatt cttcccactc atcatataga
     3241 gaggcaaggt tattttctaa attaattttt tcttggctaa cttctttagc tttatctgga
     3301 ttagagtata cttcttcttg acataaaagt atatctaatt tttcaatttc aatctctaac
     3361 ttttcaattt ctttttctat attttgtctt tttattctat ttttcttttc tatttctctt
     3421 tgttctctct cttttctttt ttcatctttt atttgagttc tagttttttc ttctttttct
     3481 tctataatac tcatctcttg gatttgcctt tttttatcta tataataatc ataatttccc
     3541 agatattcag taattccatt ttcatctaaa actaaaattt tatcaacaac agtatttaga
     3601 aagtatctat catgtgatat tgtaaatata gtcccatcat aattagttaa tgcctcttca
     3661 aggacttcct tagaatcaat atctaggtga ttagtaggct catctaataa caataagttt
     3721 gcatttgata gtataagttt aagtatggca actctagctc tttctccacc actcaatgta
     3781 gaaatcttct taaaaacttc ttcatctaca aataaaaatg cccctaacat atttctcaaa
     3841 gtagtttgag taagatgagt attattttcc caaatctcat caattattgt gttatctaaa
     3901 ttaagagttt tttgttcctg atggaagtag gaaacatgta cattagttcc aaatttaata
     3961 ttgccactaa gaggttgtag ttcattagtt attattttaa ataatgtaga tttgccaaca
     4021 ccatttgcac ctataagtgc aactttttcc cctctatata tatctaaatc caagtcttta
     4081 aaaagtattc tttctccata acccatagat acatctctaa tttgaagaac atcattacca
     4141 ctctggacag aaggcgtaaa ttgtatacga gcttttttcc tataagcttc tggtttatct
     4201 aaaacgtcta ccttgtctaa tgctttttct ttacttcttg cacgctttag gtgtttttct
     4261 ctaccataag cttttagtct ttcaattgat tcttcttgct tttttatttc tttttgttgg
     4321 tcttcaaatt tcttaagctc tagttctttt tctattttag ataactcaac aaatttagag
     4381 tagttaccat tatatacttt aagttttttg ttgtgaactt caaaaattct atttacggat
     4441 tggtctaaga aatatctatc atgagatatt aacatgacag ttcctttata ttgttttaaa
     4501 aatacttcta accattctat tgcttcagag tctaagtggt tagtaggttc atcaagtaac
     4561 aatagagttg gtttcttaag aagtaatttt cctaaaagaa ctctagtttt ttcaccacca
     4621 gaaagtatgc ttattggctt atccatatca ttgtcactaa atcctaatcc ttttagaact
     4681 cctttggctt cagatttata tccatatcca tttaaatctg aaaataattc tagtttatgt
     4741 gaatattcat ccatgatttt ttctataatt ggagagtttg tttttgtact ttcttcagat
     4801 attttaacct ccaattctct taaataggat tccatttcta tcaaattttt aaaaacttct
     4861 agcacttctt ccaaaatagt attatccgaa taaaaattgg tattttgttc taaatatccg
     4921 atttcacaat ctttagaagt ataaatatct ccactatcat atccataaat tcctgaaatt
     4981 attttaaata aagtagtttt tccagtacca ttaacaccga ttataccaat tttatcgcct
     5041 tcatttactg taaaactaat attttctaat atagaatcta taccaaaact tttatttaag
     5101 ttattacatg acaaaacaat cataaatttc tacctccttg aactatctaa aattataatg
     5161 ttttgatata atataatcaa ggttatatgt attagttttg aaaaagaaat attttattgt
     5221 gaatcaaaca aaaaagagga cacttttagg aggtgaagat gttgggaaat aaaaatatat
     5281 caatggcagt tataagaagg ctcccaaaat atcatagata tcttggagac ttattagata
     5341 gggatataca aagaatatct tctaaagaat tgagtgatat aatagggttt accgcttctc
     5401 aaataagaca agatttaaac aactttggtg gatttggaca acaaggatat ggttataatg
     5461 tagaagctct tcatactgag ataggtaaaa ttcttgggtt ggatcgacca tacaacgcag
     5521 ttcttgtagg agcaggtaac ttaggacaag ctatagccaa ttatgcagga tttagaaaag
     5581 ctggattcga gataaaagct ttatttgatg caaatcctag aatgataggt ttaaagataa
     5641 gagagtttga agtattagat tcagatactt tagaagactt tataaaaaac aataatatag
     5701 atattgctgt attatgtata cctaaaaatg gagcacaaga agttattaat agagttgtaa
     5761 aagctggaat caaaggtgta tggaattttg cacctttaga tttagaagtt ccgaaaggtg
     5821 ttatagttga aaatgtaaac ttaacagaaa gtttatttac cttatcgtat ttaatgaaag
     5881 aaggaaagta gacaacttaa acatattaat aatgagcagt tgtgaagagt attgagaaag
     5941 tcagaagatt taatattaaa tcctctggct ttttgaaata ttaggataaa aatttaaata
     6001 atgtatggaa ataaacaata ttaaggagag tggtttttgt ggaggaatta tggaaaaagg
     6061 caattgaatt tcatggtcat gaatgtccag ggttagctat aggatttaaa gcagcacttg
     6121 cagcaagaaa atatttagga tgtaaacagt caggagatga ggaaattgta tgtatttctg
     6181 aaaatgatgc ttgtggaata gatggtatac aagtcgtgct aagttgcaca atgggtaaag
     6241 gaaatttact acttaggatg actgggaaat cagtatatca tttctttaat agggaaaatg
     6301 gaaaatctat aagaatatat gtaaagtcaa agccatttca aatggataga gaaggataca
     6361 aaaagtactt attagaaagt gattttgaag aaatatttga gattacagat actaagttaa
     6421 gacttcctga aaaagcaaga atatttaaat ctcttaaatg tgaagtgtgt ggtgaatttg
     6481 catctgagca atatataagg attgaagaag gaaagaaagt atgtcttgat tgctttaata
     6541 aatatgaaag attttatgag gagtattaaa attataaatt agattaaagt taaaagtatt
     6601 agatatgtac taacagtatt ttaagatgaa ttaggtagca acataataaa atatataaat
     6661 aatattactt gaaccataca taattaaaaa gaggctgatt agtatttaga aaataaaatc
     6721 agtctcttta tgaataacga attattattc tggttgaggt gcatctacag gacaagctcc
     6781 tgcacaagaa ccacagtcta tacaagaacc agcatctata acatatgcgt cgtctccagc
     6841 tgatatacag ctaacagggc attcagcctc acaagcacca cagcttatac aagcgtcagt
     6901 aattttatat gccattttga aatcctccct tttatatgta ttgtaattca ataacattat
     6961 aacaaatatt attgaatata acaattaaat taattctaca acaaaaactt tattatgata
     7021 ttcttgtttt aaagttttac aataaagttt aatctgctta tatttttatt tattttaaag
     7081 aagtttattt taaaaataaa attaaattta aaaagttatg tattgattat atttaaaaat
     7141 gttatactct aaaaaagtat attttatttt aaaatttatt aatacaaagg agatttacta
     7201 tgaaaaataa attagtttac atatgtatta tgagtctatt gctatttgta gtaggatgtt
     7261 ctaataatcc aaataaggaa agtaccatta aaaaagactc taagaataac caaagccaag
     7321 ttaaagaaga aaaaagagaa cctgtgaaaa aagatataac tataagtttc gctggtgatg
     7381 taactatggg gaactataaa ggttcatcat attatggcag ttttgaccat gaatttaaaa
     7441 gacaaggaga agattacaat tattttctta aaaatgtaaa acctgtattt gaaaaagatg
     7501 acttaacagt ggttaattta gaaggtccac ttactacagc gtcaaatgca aaagaaaaaa
     7561 aatttgcatt taaaggaaag cctggatatg taaatatttt aaaatctgga agtatagagg
     7621 cagtaagtat tgcaaataat cattcagaag attattttga agaaggaatg aaagatacac
     7681 agtttatatt ggatgagaat aaaataaatt attttggatt agggaaagat gctgttgtag
     7741 atgtcaaagg aattaaagtg ggtctattag ggtataatgg attatcaact gaatataatg
     7801 aaaaaaattt aaaacaaatg gaagatgata ttaagtcctt aaaaaagaaa tcggatgttg
     7861 ttgtagtgta ctttcattgg gggatagaat tagattatta tccagataaa aaacaaaaag
     7921 attttgctca ttatgctata gacaagggag cagatttagt tatggggtct cacccacatg
     7981 ttattcaagg aatagaaaag tataaaaata agtatatagc ttatagtcta gggaattttt
     8041 gctttggagg aaataagaat ccttctgata cagatagtta tatatatcaa caaaccttta
     8101 ctttttcaga taataaatta acttcaatta aagagccaaa tataatacca acctcaataa
     8161 catctagtag ctccagaaat aattatcaac caaaagtatt ggatggaagt gagaaggata
     8221 gagttttaaa taaacttgaa aaaataagca aagatttaaa taaataaaat gtaaaaaaaa
     8281 ataaatatat atatttattt taataattta tattttgttt agggtatggt aaatcgtggt
     8341 attaaataag tagattttaa tagttaaatt atagtcaatt ataataaagc tatttaatct
     8401 acttttttaa tataaaaata taagggggaa tttgtaatgg cttgtaacac ttgtaaaatg
     8461 tgcgaaagtg cagacaagaa attagaaagt tttgtagctt caaaggatgt tgaaacagca
     8521 tttcatagaa cagaagatca gaaagttaag tgtggttttg gacttcaggg ggtttgttgt
     8581 agactctgtt caaatggacc atgtagagta actcctaaat caccaagagg tatatgtgga
     8641 gcagatgcag acactatagt agcaagaaac tttttaagag cagtagcatc aggagcagct
     8701 tgttatttac atgtggtaga aaatacagct aagaatttaa aaaatgttgg aataactaaa
     8761 ggtgttgtta aaggagaaaa aacattaaat caattagcag agatgtttgg aatagaaagt
     8821 gatgaaaagt atgataaatg tataaaggtt gcagacaaag ttatcaacga tttgtataaa
     8881 tcaagagatg ataaaatgga attagtagaa aaaatagctt atgcacctag agtgaaaaag
     8941 tggaaagagt taggtataat gcctggtggt gcaaagtcag aagtttttga tgcaatagta
     9001 aaatcatcaa caaatttaaa tagtgatcca gtagacatgc tagttaattg tttgaactta
     9061 ggtatatcaa caggattata tggtcttaca ctaactaatc ttttaaatga tgttatgttg
     9121 ggagaaccag ttataagaat ggctcctgta ggatttaatg taatagaccc agactacata
     9181 aatataatga taacaggcca tcaacattca acatttgcaa atttccaaga tagattaaaa
     9241 gatgaagatg ttataaaatt agctcaatct gttggtgcaa aaggctttaa gctagtagga
     9301 tgtacatgtg ttggtcaaga tttacaatta agaggagaac attatcaaga agtatttgct
     9361 ggacatgcag gtaacaattt tacaagtgag gctgttttat caactggagc aatagatata
     9421 gttctttcag aatttaactg tactatacca gggcttgaac caatagcaga taaatataaa
     9481 gtaaaaatgg tttgtttaga tgatgttgct aaaaaagcga atgcagaata cataggttta
     9541 gacagaagta aattagatga acttagtaac actttaatag aaaaagcatt ggagtcttat
     9601 aaggagagaa gaggaagtat tgagatagat ataccaaaag accatggatt tgaacaatct
     9661 ttaactggtg taagtgaaaa gaacctgaaa gcgtttttgg gagattcatg gaaacctctt
     9721 attaatttaa ttgcagaagg aaaaattaaa ggtgttgcag ctgttgtagg atgttcaaat
     9781 atgacagcag gaggacatga tgtaaatact gttgaactta ctaaagaatt gattaaaaaa
     9841 gatataatag tactatctgc aggatgttct actggaggat tagaaaatgt tggtcttatg
     9901 tcacctggag cagaagaatt agcaggagaa aacttaaaag aagtatgtaa gactttagga
     9961 ataccaccag tattaaactt tggaccatgt ctagctatag gaagactaga aatagtagca
    10021 acagaattag cagctgaact tggaattgac ctaccacaat taccattagt gctttcagct
    10081 ccacaatggt tggaagaaca agcattagct gatggagcgt ttggtcttgc ccttggctta
    10141 cctcttcatt tagctttacc tccatttata acaggaggaa aattggtgac agaagtatta
    10201 acagaaaaat taaaagactt aacaggagga catgttatag tgaatccaga ccctaaatct
    10261 tcagctaatc aattagaaga aataataata gatagaagaa aaaatttagg gttaaaggat
    10321 gtggaatgca atgcatagaa tttttataaa taaagattta tgtacaggat gtaagagctg
    10381 tgtactagca tgtatgctaa aacacaataa agattatgac atgtacactc ttgatttaga
    10441 aaatatagat aatgatagta gagggcatat agaattagat agtaaacata ataatccagt
    10501 tccaattctt tgtagacact gtgatgaacc tgaatgtgta ttagcatgta tgagtggtgc
    10561 aatgcataaa gatagtgaaa gtggaatcgt ttcatatgat gaagagaaat gtggttcatg
    10621 ttatatgtgt gtaatgtctt gtccatatgg attattaaag ccagatgata gaagcaagca
    10681 aaacatactt aaatgtgatt tatgtaaaga tgaggagtat cctagatgtg tagctaattg
    10741 cccaagtgga gcaattgaat tacagaagga ggaaaatgat gaattatgta gtgttagggg
    10801 ctagtgcagc tggtataaat gcagtaaaaa cattaagaga attagataaa gattcaaata
    10861 tagtagtaat ttctaaagat gaaaacgtat attcaagatg tatgttgcat cacgttatat
    10921 ctgaacacag aactttaaaa caaataaatt ttgttgatga agattttatg gagcaaaata
    10981 atgtaaaatg gattgcaggt aagactgtta aaggtataga cataaataaa aaggtagttc
    11041 aaacagaaga cattactgta aattatgata aacttttaat agctacaggt gcatcttctg
    11101 ctattcctcc tataaaaaat ttaagagatg gaaattttgt atattctgtg agaaacatag
    11161 atgatattta taaaataaaa gaaaaagcac aaaattctaa aaatgtagtt ataataggtg
    11221 ctggtttagt tggtatagat gcattagtgg gattatttaa atatgagcag ttaaatattt
    11281 ctgtagcttt tatggaaaag tacattttag atagacaatt ggatgaatat acagcatcag
    11341 tgtatgaaaa taagttcaaa gaaaaaggtg taaaatttta cccaagtgca tcaatacaag
    11401 aaattgtatt ggataacagt aaaaatgtta caggtgttgc attttcaaat ggtgaagttt
    11461 tagatgcaga catggtaatt gtagctacag gggttaaacc aaatgctgac tttttagatg
    11521 ggacaggaat agaatatgat agaggtataa taatagatga tatgtgccaa acaacacaaa
    11581 aagacatata tgcagcagga gatgttgtag gtaaaaatgc tatttggcct ctggcagtaa
    11641 agcaaggaat tgtagctgcc tataatatgg ttggaaaaga taagaagata gaagatgaat
    11701 ttgcttttaa aaatagtatg aatttcatgg atataccaac tatttctata ggaatgaata
    11761 cacctgtaga tgatagctat aaagtattaa ccagacatgg aattaatgat tataagaagt
    11821 ttgtttttaa agataatgta atctacggag cagttataca aggagatatt tcatatgtag
    11881 gtgtattaac ttatttaata aaaaataaag ttgaaatata tgatttagaa aataggatat
    11941 ttgatgtatg ttatgcagac ttttttaata taaaagaaaa tggagaattt tgttatagtg
    12001 tatagtatat acgtggagta aggtttataa atacagtaaa tcaataaata gagttattct
    12061 aaaataagaa aaatgacaaa tcttatttag aaatagctct attttttgta attaaattta
    12121 aattatattc aatttgtaaa atattatttt gaacaattat acatatactc tctagttaga
    12181 ggtaaatcat tttttatacc tttagtgaaa agtatttgat gtatatctat aataccagtg
    12241 ttgaaagcag cagcacaaga acaaaggtat aaatcccaca ttcttataaa cttatcatca
    12301 aacatttctt taatctttgg aagattctca gagaaacttt tttgccagta tagtaaggtt
    12361 tttacataat gtaatcttaa actttctacg tctatagtat gatatttgta atcactacaa
    12421 atatgtatca tttctcttag gctaggaata actcctcctg ggaaaatata tttttttatc
    12481 caagggtctc cataagattc ctgcaatcca cttatatagt gaagtaaaaa caatccacca
    12541 tcttttaaaa cagcatttac gtttttaatg aaaagttcat agttttctct accaacatgt
    12601 tcaagcattc caacgcttac aactctatca aacataaggt ctgatttttt taattctcta
    12661 tagtccataa gtcttacctc aagatagtct tgaaggttct cttctttaat tcttgcgtta
    12721 aattccttaa attgctcttc gctaagtgta attccaacgc cgtgaatttt atattttttt
    12781 gcagcttcta ttagtaaaaa tccccaacca caaccaatat cgagaaggct cataccttcc
    12841 tctaaattta gtttagttaa tatgtggtga actttattga cttgagcatc ataaagagta
    12901 tcattatcat ttctaaagta tgcacaagaa taactaagtg tcttatctaa ccataagcta
    12961 taaaaatcgt tgccaatgtc atagtgggca tgtatttctt tcttctgatg tctcattgaa
    13021 tttgatgagt gcaatagatg ttttacagct gaattatcaa tattgaattt gtctatttgt
    13081 tttaaaagta gatttaaagt taagaataaa tctccctcaa cttcaatgtc tctatttatg
    13141 taggcttctc caagtgctaa agatgtactt tttaatagtt cagacttgct aatatcatta
    13201 tttactataa ctttgaactg aggggagccc tctccaatta gaaattcttc ctcatcttta
    13261 agcctaacta gaaatggtgt ttcaccaaat ttctctaaat agtcttttaa aaaatttttt
    13321 gtaatattca tattaccagc tccttctaat gtaaatccag taagtttccc tgcttaaaat
    13381 atattatagt ccttttttta aaaaagtaaa attttttcaa taaatttttt atttaaagga
    13441 aatttatttt ttataaagaa atttcatgtt gtagtatatt ttttgcttat aaattttggt
    13501 actggatata atattaaact gttaatgata taataaattt aatctataaa gtaaataaaa
    13561 ttttgtaata aatagttaaa gctattttat aaggagatat atgatggaag tatatgcttt
    13621 ggttgggtca agtggcacag ggaagagctt taaggcttta gagttcgcat atgaaaatga
    13681 tatagaatat ataatcgatg atgggatttt aatatacaaa aacaaggttt tagctgggat
    13741 atcggcaaaa caagcaaata ctactataga agcagttaaa agggctattt tttataatct
    13801 ggagcataga caagaagtta gagaagcaat taaaaaagaa aatataagaa aaattcttat
    13861 tattgggaca tcaaaaaaaa tggttaatca aattgtagag agattgtcaa ttggaagggt
    13921 ctgtaagttt ataaatatac aagatataag tactaaatat gaaatagaaa tagcaaaaca
    13981 agctagaaaa gaaggaaatc atgtaattcc tgttccagcc gttgagataa aaagtatggc
    14041 aagtggtctc agtataaatt ctttgaagag actttttaga aaaggaaata ataggaatat
    14101 ggtagtttta gaaaaaacaa ttataagacc aacttttagt tatataggaa agttttatat
    14161 tagtgctgat gtaattaaac agattattga atatgaagta tacaactttg gaagcattga
    14221 tagaataaat aaaatcaata tagagaattg caataatttt atgaatattt ttgtaagtat
    14281 aaatatcaat gatttagaaa ctataaaaaa aattgaagac atacaacaga caataaaaaa
    14341 gtctattgag aaaatgactt tagttaatgt acaaaaaata gatattaaca ttcataaaat
    14401 taaaaaaata aacttcctcc aatgttaaaa aataaactga gaaaatgata tactaatttg
    14461 caataaagtg tgctataatt aaactgtaaa tttaggaaat ggttttttct aaatggagta
    14521 tacataatta taacgtattt ataagggggc tttcaaatgt caggaaaaga tgtaaatgtc
    14581 ttcgagatgg cgcaatctca agtaaaaaat gcatgtgata aattaggtat ggaaccagca
    14641 gtttatgaat tattaaaaga acctatgaga gttatagagg tttcaattcc agtaaaaatg
    14701 gatgatggtt ctataaaaac ttttaaagga tttagatcac aacataatga tgcagtaggg
    14761 ccaacaaaag gtggaataag attccatcaa aatgtttcaa gagacgaagt aaaagcttta
    14821 tctatatgga tgactttcaa gtgttctgta acaggtatac catatggtgg aggtaaaggt
    14881 ggaataatag tagatccatc aactttatct caaggtgaat tagaaagatt aagtagagga
    14941 tacatagatg gaatatataa attaataggt gaaaaagttg acgttcctgc accagacgta
    15001 aatactaatg gtcaaataat gtcttggatg gttgatgagt acaataaatt aactggacaa
    15061 agttctatag gtgttataac tggtaagcca gttgaatttg gtggttcttt aggaagaaca
    15121 gctgctactg gatttggtgt tgctgttact gcaagagaag ctgctgctaa attaggaata
    15181 gacatgaaaa aagctaaaat agctgttcaa ggtataggaa acgttggttc ttatacagtt
    15241 cttaactgtg aaaaacttgg tggtactgtt gtagctatgg ctgaatggtg taaatcagaa
    15301 ggttcttatg ctatatacaa tgaaaatggt ttagatggtc aagctatgtt agattatatg
    15361 aaagaacatg gtaacttatt aaacttccca ggagctaaga gaatatcttt agaagagttc
    15421 tgggcttcag atgttgatat agtaatacca gctgcattag aaaactctat aactaaagaa
    15481 gttgctgaat ctataaaagc taaattagtt tgtgaggctg ctaatggacc aactactcca
    15541 gaggctgacg aagtatttgc tgaaagagga atagttctta ctccagatat attaactaat
    15601 gctggtggag ttacagtttc ttacttcgag tgggtacaaa acttatatgg atactactgg
    15661 tcagaagaag aagtagagca aaaagaagaa atagctatgg ttaaagcatt tgaatctatt
    15721 tggaaaatca aagaagagta caatgttaca atgagagaag ctgcttacat gcattcaatt
    15781 aagaaagttg ctgaagctat gaaattaaga ggatggtact aagatttact cttaataaat
    15841 aataggatat aattatttaa gatataaagg ggctgtacat agtacagccc ttaaattatg
    15901 tggcttacaa tgaagtaagc tatttttgtt ttaacttata aataacttga taaattatgt
    15961 aatttagaat gcataaataa gatgggtgaa aattaatggt tgatttaatt ttgaatgtat
    16021 tttttaccac cacatgatta caaattagag gagttagatt ggatttttgg acatgaactt
    16081 atgtatttta aaagcaaaga ccttcattta aagtatctag tattattttt aaaaatagta
    16141 tattggttta atccatttat atatataatg gaaaaatata tggatttgga ttgtgaactt
    16201 tattgtgatg agagagtttt aaaaaattgt actgtacaac aaaagcaaga atatgctcta
    16261 actattgtaa attcaatgaa aagaggtata aatttttcaa ataaatttgt aactggatta
    16321 aataaacaat ctgatattag aaaaagagta atgaacatgt tcagtgaaaa acataaaaat
    16381 ggtatcttaa tggctttgat tataagcttg ttttcttcta ttacattttt taaattcgat
    16441 tcaactagtc ttattgaaga gaatgatttc ttagagaggt caagatatgc gtttgaagga
    16501 aggcaagaag acatgtttgt aacaatagat tctacatatg cagatgcacc tataaaactt
    16561 agaaaagagc atgaagaaaa ctgtagagtc aatggaatag taccaaaaga ttcagattca
    16621 atagaaatta ttcctagatt ttataaagat tgtatggatt taaaaaaata agttagactt
    16681 aatattgatg ttgatattat gaatttttta tatttaagca attgaaaatt ttattataga
    16741 ttcaatttta tatgtgttta agtatgaaat ttatagatag ttgaaataat taaatatgat
    16801 tgatgcatag gaatcattac taagctataa ttaattatag gggagtttaa ataaattatg
    16861 agttacacaa ttataaaatg ataaaaaata gttatctgtt acaaaaaatg tatcttttta
    16921 attaataaag ataatatgaa aacttaaaag g
//
LOCUS       DAEPOZ010000064        16583 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.064, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000064 DAEPOZ010000000
VERSION     DAEPOZ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16583
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.064"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..663)
                     /locus_tag="KR440_001606"
     CDS             complement(<1..663)
                     /locus_tag="KR440_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HBH0698661.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQE"
     gene            complement(839..1369)
                     /locus_tag="KR440_001607"
     CDS             complement(839..1369)
                     /locus_tag="KR440_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698662.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEIVKNVDCN
                     IVQYSNKDERRVKAKANKMAMRLIHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(1386..1616)
                     /locus_tag="KR440_001608"
     CDS             complement(1386..1616)
                     /locus_tag="KR440_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698663.1"
                     /translation="MSKLVSKVKKDLEIKWLGVDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMAVVIVIFFDYIYRVLKMTRKK"
     gene            complement(1631..3025)
                     /locus_tag="KR440_001609"
     CDS             complement(1631..3025)
                     /locus_tag="KR440_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBH0698664.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTI
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(3151..4404)
                     /locus_tag="KR440_001610"
     CDS             complement(3151..4404)
                     /locus_tag="KR440_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0698665.1"
                     /translation="MENKSNFKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALSLS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGVLGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(4455..5609)
                     /locus_tag="KR440_001611"
     CDS             complement(4455..5609)
                     /locus_tag="KR440_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBH0698666.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRKLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIKKLK"
     gene            complement(6168..6737)
                     /locus_tag="KR440_001612"
     CDS             complement(6168..6737)
                     /locus_tag="KR440_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH0698667.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            6940..7167
                     /locus_tag="KR440_001613"
     CDS             6940..7167
                     /locus_tag="KR440_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBH0698668.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(7386..8129)
                     /locus_tag="KR440_001614"
     CDS             complement(7386..8129)
                     /locus_tag="KR440_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBH0698669.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNAASQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYITDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            8297..9169
                     /locus_tag="KR440_001615"
     CDS             8297..9169
                     /locus_tag="KR440_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH0698670.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            9169..9834
                     /locus_tag="KR440_001616"
     CDS             9169..9834
                     /locus_tag="KR440_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBH0698671.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            9855..10712
                     /locus_tag="KR440_001617"
     CDS             9855..10712
                     /locus_tag="KR440_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0698672.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGSYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(10868..13414)
                     /locus_tag="KR440_001618"
     CDS             complement(10868..13414)
                     /locus_tag="KR440_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBH0698673.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMTNGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYTSSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVIAKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(13414..14100)
                     /locus_tag="KR440_001619"
     CDS             complement(13414..14100)
                     /locus_tag="KR440_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0698674.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(14183..15172)
                     /locus_tag="KR440_001620"
     CDS             complement(14183..15172)
                     /locus_tag="KR440_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0698675.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIVCEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(15159..15845)
                     /locus_tag="KR440_001621"
     CDS             complement(15159..15845)
                     /locus_tag="KR440_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0698676.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(16345..>16583)
                     /locus_tag="KR440_001622"
     CDS             complement(16345..>16583)
                     /locus_tag="KR440_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698677.1"
                     /translation="IEEPRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKMRRTAIEF
                     STEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
BASE COUNT         5761 a         2897 c         2153 g         5772 t
ORIGIN      
        1 ctcttggttt ataaaataag ggcttgtcgc aattgttatt actttggctt ttttaattaa
       61 gttttttatc ttgtaaaatt tgagttcaaa tggtatatag tccatatcac ttgaaaatat
      121 atccaaatct atatcaagaa caaactctga ttctacttct aaatcaaatc catacagact
      181 atctattatt attaattctg aaaatatgtt atattttaaa gcaggtttta taaaacttcc
      241 cacatttaaa acttcatttg tatacctaaa tacatctttt agattattta catctacatc
      301 atagttctca ggctctctag tatccttatg ttggtctaca tgcactaact tgcatccttt
      361 tgtaaattca ccctttaaca aagattttgc ccaaaaataa aatgcatggt tatgattgtc
      421 aaacacatat atctttttat ttcccaggac atattcgacc atattctcca atccttttgc
      481 ttttatttcc ttattttcat ctacttcatt aaataccacc tcattgccta accttacatc
      541 atttaatgta ccatttataa gttttggaac gtatatgctc ttattttctc ttttatcata
      601 tgaaaataca ttatttccta ctggtttttc tatataaaat ccactatatt cataattttc
      661 cataaaactt tatttcctcc attgttatat aaatatataa gttataattt taaaaattaa
      721 aatgcactta ttgtaaatca tattatctat tatagaaaac atcttatata actaaatata
      781 atatcttctc tcaatatgta ttatacaata agtgcaaaat atatttatat aaaatatact
      841 acattcctat aacaaaacca tcagcttcaa ttggaaggta actaactctt ctattcaact
      901 ccacctttcc aaaaaatact acccaatatg gtctaaaaat actttcaatt ttaactattt
      961 caatctctgg gattcctccc ataaacctat gaattaatct catagccatt ttatttgcct
     1021 ttgcttttac tcttcgttcg tccttattac tatactgcac aatattacaa tccacatttt
     1081 ttacaatttc atatgtagga aaatcttgta ctatactacc agaacctgta gaaccattca
     1141 ctaaaatttt taattcattt atatcattag aatatctcct attcttgaaa atggatttcg
     1201 ttttgtgaat tttaagtgtg ataattttaa actctatata atgaagtcta acttcatgag
     1261 catatttgtt ctttaatatt agcttactaa ataaagaatt tttcccactt ttagctatct
     1321 ctatagcttc ttcttttgta attcttatag gtaacgctct tatatccata aaatcattct
     1381 ctttcttatt tttttcttgt catttttaaa actctatata tataatcaaa aaatattaca
     1441 attacaactg ccataactcc atatggaatt atttttccaa ctataatatc atttctagtc
     1501 aatataccat aaaatataaa tcctgttatt aaaaagtaca ataatactag aaaaccgtct
     1561 actccaagcc attttatttc taaatctttt tttaccttag acactaattt agacatagca
     1621 gtttctcctt ttatagatta ttttgtgtat ttaaattttc acctattcta ttaccaatca
     1681 tccatgcaat aaatgaaaaa ggtatagcta tcacaagagg ctctaactca aagctctgaa
     1741 ctggaaatat tttaaaataa ccaaaagtca ctccacctac aatcattcct aaaataccac
     1801 ccatagatgt tcttcttcca tttccaagaa gcccacctac tataggtaca aatgccgaac
     1861 ccatccaaat acttcctaca aatatacatg catcatatac attttcaaat ctaaatgcta
     1921 cgctaagccc cataactgct gctaaacata cacctattct agtaaacttt actaattgtt
     1981 tctgagataa ctcttttttt gtaattcttc catatatatc atttgctaat gtagctccag
     2041 ctgctagata atagctatcc aatgttgata ttatagaacc taaaattcct attataaata
     2101 ttactcttat tccttgtggt aatgtatcta aaactaaagt tatatatcca gcggctggag
     2161 ccatctctgg ataagtagct cttactataa gtcctgttat agttaacacc atgtcaaaac
     2221 atatccatag tgaaaaacaa gttaataagg ctcttcttcc aaccttagga gaatctgctg
     2281 ctgaaaatct ttgataaaaa gttgggtctg aataagctcc aaaagaatat acaattaaca
     2341 tcaatgcttt catagctgac ataccttttg tagggtgcat aagctgtggt gcatctttga
     2401 gtgcttctgt gagaccttga aatcctccta tttgttcaaa ttgacctggc ataaccatag
     2461 ttaaaccaaa taccatacaa aaaaacaata ccatatctgt aatggcaact cccataagcc
     2521 caccaagtac tgtaagtaca acgactactc ctaccattat taatcctatt aattctttat
     2581 ctattttcca tgctacgtta cctatagttc ccatagcagt tatttctcca agttgtgcac
     2641 aatatataaa catcaaaatt gccccaatta tagcaacatg cttaccataa aaactttcca
     2701 gtaaatcagg aactgttata ttggttctta tatgaatcct tggaccaacc cacaatgcta
     2761 aaggcattct cccaatatgt gcagctattg accaaactgc ccatgcagat attccatata
     2821 tagccgcata ctctacacta cctactgtac caactcctcc ataccatgat gcaaccaaag
     2881 ttcctactat taaggaccat ggtaaacttc gatttcctag gtaataactt tccatatctt
     2941 ttacatttct agaaaaataa taacctattc ctaataatat aaatgcaaat ataactataa
     3001 tcccattttc ccaaaatgtt aacatatcta atctccacat tcttatattt taatttttaa
     3061 ctttaatatc taaatatgaa caactctacc catttccata aaaataggta gagttatata
     3121 ataattataa gtatttaagt aatcttgtta ttaagcaatt ttattgttta aacctttatt
     3181 ttctctttta tttattttat ttaaagcata agtagcaaat atccctataa ctgcacatac
     3241 tacagataaa ataaatatat atgtatatcc tgtttgtcca tatttatcta accaattacc
     3301 tgcaatagtg taataaaacg catctggaga aaatcctata aatgaagcaa atccaactac
     3361 tgctcctgta gtttctaaag atatattaga ctctgaaaca gatgcaaagt atatacctct
     3421 aaatccaaat aaaattacac ttagtattat catgtttatt acagctatcc atataaatcc
     3481 tgtactctta ggaataacca agtaagcagc tgtacttact gtcattaata taaatcctac
     3541 aaacattact cttatagatg aaccaacttt atctgttata acaccagcaa ctggagatgc
     3601 tcccattttt ataacataag ttcttattat tgataaagca gaaactagag tcattggcat
     3661 tacataaact tctgataagt atggacttaa ataagttaaa cttgagaata ctaaataagt
     3721 tgaaaatatt ataagaccaa ttaaccaagc ctttggcatt gtaactactt ttaacaatga
     3781 cttaatactt aatccttcat ctgatgctcc actatctttt ttctcttcca ctaagaaaaa
     3841 tagaagtata ccacatatta tagatgctgc agaatataac catactacat atttgaatcc
     3901 tactgttatt tcagcaaatt taccaaagaa gtaaagacct ataaaagaaa ttaacgtacc
     3961 tgatacacct gataaccctt cataaaaacc aaatattctt ccttgttctg actcacttcc
     4021 ttgcattcta acaaccttaa cagatgctga gaagaaagtt aatattgttg taaatcccca
     4081 taatacaaat attaagagta tcatactata tgatgggaat gtagagaaat aaaaaccaag
     4141 tactcctgaa gcaattaaag aaaaagttat cagctttcta accgagaatc tatctgcaaa
     4201 ccatcctcct ataaaataag ataccattcc tactaatcca tataaactca ataagttccc
     4261 tatttgtgta tggctcaatg ataaagcttg ttgcattggg tcataaaatg atgatttcat
     4321 atatggtaaa ctatacatag ccgttgttcc aaatgccaat atgaatatta tcaagaatct
     4381 tttaaaatta cttttgttct ccattaattt tctctcctta aaattttatt gtttataatc
     4441 tcattcatgt aattttattt taattttttt attgcttgtt taaagtcatt tattatatca
     4501 tttgaagatt ctaatcctac agctattctt attagatttt ctccattcat ttgaactaat
     4561 gtatgctctt cccctaagct tgttgctatt gttgcaagct ctaaagagtt cattaatttt
     4621 ctactagctt ggtctcttgt caatccattg ataccatcct taagtttaaa tgaaactaca
     4681 cctccaccta aaccattcat ttgagtttta caaagctcat attgaggatg agattttaat
     4741 gctggatact ttaccaattc cacattctct tcttcctcta aaaactctgc tactgctaat
     4801 ccattttcac aatgcttttt catcctcata tctaacgttt tcatacctct taaaatcatc
     4861 catgcactca ttggtggtaa agatgctcct gtaaaacaac ataaacttgg ccatctaact
     4921 aaatctatta attcttttga acctgctact gctcccccta aagtatcccc atgtccattt
     4981 ataaacttag taagactatg tattactaaa tctgcaccta aatctattgg tctttgtaca
     5041 attggtgtcc caaaagtact atcaactata gctaagcttt tattttcatg agcaatttct
     5101 gctattgttt ttatatctac taagtctaat gatggattta aaggactttc tatataaact
     5161 attttagtat ttggtctcat tgcttctttt acattttgtg gatttactgc atctacaaat
     5221 gtaacttcaa ctccaaattt agataacaac tcagacatga ataatttagt gtgtgaaaat
     5281 attccatctg aactaactat atggtcacca ctttttacca atgctaatat agtagatgtt
     5341 attgccccca tccctgatgt actagatatg gcactttctg cattttcaag catagccaac
     5401 ttagcttcta actctgtaag agtaggatta cactctcttg tatacacata tccccatgtc
     5461 atacacaact cttcaaaatg ctctactgtc tttgcagcaa atgttgctgt ttgacatatt
     5521 ggtgtagcca atgcacctgt tgttgggtct gcactatgtc cccaatgtat tatttttgtc
     5581 tctaaatctt gattaatatt gttactcatt ttgatttcct cccattttga cttatttata
     5641 caacgttttc cctgccaaat ttaatcaatt tagcttagtc ttaatacatt ttttcaagta
     5701 tactttttta tttgttataa tttctaaaaa tatgacactt attttgtttt tttacagttc
     5761 taattataaa ctctatagta actatagagt caatatgttt ttgatatttt ttttacaatg
     5821 tttaaaatag tcagtacaaa aataggtata tcttagaata actaaagtca tcctaagata
     5881 tacctatttt tatatcacaa tataaataat tacatataag cattagtttt tttatcataa
     5941 atagattttt caagatttat catcttttca taaatatatt atagttaaat attttaatat
     6001 tacctaacat ctacaataaa atatactctt aaaataaaat tatataatta aaaatactct
     6061 ataaaagcta aaaagttttc tattcgctac atctaaaaat aattagatag aggtataaat
     6121 gtatttagtt tatttatacc tctagtcttt tataaattat atctctacta aattgatttt
     6181 ttagcacgaa taagtgtgct cttggagata ccagtcatct tcacaacctc tctatatggc
     6241 ttagaattta ataaccccaa tgcatattgt atctgttgat tgctgtattt ctttggccta
     6301 ccatctttaa agtccttttt ctttttagca aattcttttc ctaattgagt acgttctatt
     6361 atcatatttc tctcaagctc tgcaactgca agtaatgtag tcaaaaagaa tttacccatt
     6421 gaagtatttt ctaaaagacc tatatttaaa atatgaacac ttacttcttt agcaaataaa
     6481 ccttctatta gttgtatccc ttctgatgta cttctagcta gtctatccag cttagttgca
     6541 acaagtaagt ctcccttttt tagagatgat ataatttttt caaatttagg cctgtttgta
     6601 gtagtgcctg tataagtttc ctttattatt ttagcatcat tatatttatt caaaatttct
     6661 tgctgttgct gatacaaact gttgttgtct gtttgatttt ttgtacttac acggcaatat
     6721 ccatatataa taatcatcac ttcctaacat aaagattttg tacagaagtt acccagaagc
     6781 ttgtttttac ttatctttat ccattttact aaaacgattt ttgggtgtca atactgttaa
     6841 cttttgacac cataataatt cctttataat ttgtttgact atgtcaaaaa ttaccaatat
     6901 aatgaaatta atgagtttat acaaattgga ggaaataata tgaatagtct gttaaaagaa
     6961 gcaatagatg aattaaaaaa catccattgc ggggaagttt ttttattaaa agaattgttc
     7021 aaaggctatg aatggaacag gttggagatg aatattagat tgaaattagg aaaacttttt
     7081 ttgtatgaag tggtaactaa tcccaattta aatgtagagg tcatcaataa aaattctgct
     7141 tatcagcaaa tgtataaaaa aaaatagtga cttaaatgta tataatagta taatcttctt
     7201 attgattcaa aagttaaaac ttttaataca aaacaatcat tctattttaa catttatcaa
     7261 tatgtctaaa tttatataat taacacatac gaatagtatt gtttaaatca cattatttaa
     7321 gatagataaa tcttaaccct actccagcat aaatactaaa atagggctta gaattatcta
     7381 tatttttatc ccataaaatg cttagctctt cctcttgatt gaacatatat atctggggaa
     7441 atacaataga agcgtaaatt tataaaattt ccaaagtcag aaaacttcag ttcaaactta
     7501 ggtataccaa aactttcagg tgctatttca tcaacgccaa aatagatgat aattccatta
     7561 tctgtaatat aaaatgcttg gtcttcaggt ataactaact caacattaga ataatacatt
     7621 tctctatttt catttatttg agatttaatg taatcagtaa ttatattaat ataatcaact
     7681 ccatctctaa aaatgtcatt aagtaaaagt tcttttccag ttgtcaaatc tatattgtaa
     7741 ttattcaatg tattgtattt aggcccatag ttattagcaa accccataag gtttaatatc
     7801 atactcagta cataattttt attaaaagta actgcgtaac tggtagatac ccaatagctt
     7861 tgttttggaa gtgaattttg agaagctgcg ttattatatt ctcgttcttc ttcctgtaag
     7921 ccatttttaa aattatttac agtcattctt acagcattgt ttatgttatt taaaataact
     7981 gggtttacat acgtggattg atttctaata ttgtatataa caggatactt tacatcatat
     8041 gaaaaatact tacttctttc aaaatcttca ttaaattgta catataatat attatatgta
     8101 caggtatttg tagattgata attttccatt aaaatcctcc cccttttata aaaaaatcag
     8161 atacaatata atatgcttaa aacagtaata atgttaatag taaatgtaaa gaatagaaag
     8221 tatctaaaac agcaaaaaat gactaatttt gtggtataat acataggatt gagagttatt
     8281 ttataggagg aaaaatatgt tattattagc agataaatgg aaagattatg aacttataga
     8341 tatgggaaat ggagaaaaat tagaacgttg gggaagttat gtacttagaa gaccagaccc
     8401 acaggttgta tggccaatgg aatctgaatg ggctttatgg aaaaaccctc atggacatta
     8461 tcacagaagc aataaaggtg gaggtcaatg ggaacacaaa aaaagatatc cagaacaatg
     8521 gactataaat tataaaaact taaagtttca cattaaacca actggcttta aacatactgg
     8581 attattccca gagcaagcag ctaactggga ttggatgata gagaaaataa agaaagctaa
     8641 cagacctata aaagtgttga atctttttgc atatacaggt ggagctactg tagcatgtgc
     8701 atctgctggt gcagaagtat gtcatgttga tgctgcaaaa ggtatggtta attgggcaaa
     8761 agaaaatata gaattatcag gtcttaaaaa tcaaacagta agatttatag tggatgatgt
     8821 tgtaaagttt gttgaaagag aaataagaag aggtaaaaaa tatgatgcaa taataatgga
     8881 ccctccttct tatggaagag gtcctaaagg agaagtatgg caaatagaag aaaagttata
     8941 cagttttgta gatttatgta tgggagtttt atcagataac cctttatttt tcttaattaa
     9001 ttcttataca acaggatttt ctccaatagt tcttgaaaat gtattgtcta caacaatagg
     9061 taaaaagaca aatacaggag atatatacgg tggggaatta ggaataccag cttccagaga
     9121 tggtaagata cttccttgtg gtatatttgg aagatgggag aaaaaataat gataaaagtc
     9181 atatatgaag ataaccattt attagttgta gaaaaaccag ttaacatatt gtctcaagga
     9241 gataatacta atgataaaga tatggtaaat cttttaaaag actatattaa aaaaaagtat
     9301 aacaaacctg gaaatgtata tattggttta atacatagat tggatagacc agtaggtgga
     9361 gttatggtgt ttgcaaaaac atcaaaagca gcatctagat tgtctgagca ggtaagaagt
     9421 aagactttca aaaaaactta tctagctgtt gttcatggca agataaaatc taactcagat
     9481 atactaaaag attttttata taaaaataaa aaaactaata tggtaactgt agtgaaaaaa
     9541 agccataaag atgccaaaga agctgaactt aactataatg tattagattt taaagaaaat
     9601 ctaagtttag ttcaagttaa tttaaagact ggtcgttctc atcaaataag agttcaattt
     9661 gcaagcagaa aacatccatt atacggagac caaagatatg gcaaagaaat aaataagatt
     9721 ggacaacaaa tatctctttg gtccaataag atagaaattt gccatcctac tacaaaagaa
     9781 aaaatggaat ttacatgtca tccacctgac aaatatccat ggaatatatt ttaaatctca
     9841 aatgaggtga ttatatgagt aaaaaatttt caataggaga aatgtctaaa cttcataaaa
     9901 taagcataca gacactaagg tactatgacc aaatagattt atttaaacct gttcatgttg
     9961 accaagaaag caattacaga tattactcaa ttgaccaatt ttcacaatta gatattataa
    10021 aatatttaaa atatttaggt atgcctttaa aagaaattaa gtcaaagctt gatgaaaata
    10081 atgaagaaat tctaaaatta ctaacaaata aaattaatat agtggatgaa aaaattaaag
    10141 aattagagct tataaagaag gtactaagca caaagaaaga aactatggaa aaaggtatga
    10201 aagtagatga agttggtaaa atagtgagaa aacatattcc acgaagaagt atcttatcta
    10261 tagagtatga agaaggatta agtagtgaag aaactatgga actttccaga agacaaatag
    10321 caaatatatt agaagataat atatctgtgt tctatggtgg agtaagtgga cttgtatcta
    10381 tagataaatt tttaaaagaa gataaagtta tttatgacaa tagttttgtt actgttgaaa
    10441 gagacttatt tgatataaaa tcacaaaaac atataacaga aatttcatct ggtgatttta
    10501 tttgtatgac atataaaggg tcttacaaag aaaattatac cgcttttaag aaactggttg
    10561 attatgttga atataaaaga atacctattg aaaattatat atatgaaata cctataatag
    10621 accctctgtc tacaaacaac gaagaggaac ttctaacaga aattcaaata caagttaaaa
    10681 agagagaaat tgacttaaga tgtctagtgt agtaatctag tttacttaca catcttaata
    10741 tagttgtcta ttatcaacat atgatttttc tagatagtga ttcctaacag tgagattttc
    10801 aaaaaatagg aggaaatatg gctaatgcta attccctcct atttttaatt aatccatatc
    10861 attttaatta agccatgtca tcacgaagtg catcaataat attttctttt tttatcttat
    10921 taatagtata caacattgta ataaatacta taaataaaac actaaatata ctaattccaa
    10981 tactaaacca tggaatcaca aaatcaatat catctgtaac cattacttta tgaattagcc
    11041 atgaagaaat tactgctata ggaagtccaa agaacaatgc tctcatgcca taaaaagcac
    11101 actcaaaatt catcatcttt tggaaatcat gctcagacat tcctacagag cgaagcatgg
    11161 caagttctct acgacgtaac ttaatattag ttgaaattgt attaaacaca ttggcaatcg
    11221 aaatcagaga aatcataatt atgaaagtat aggcaaaaac attggcaata aaaatgtaat
    11281 tacggttctc atccatcatt ccatacatat tgtacagatt gtactcatta gtaactccct
    11341 cactctcaat aatttgctcc atttctgcaa ctgactgagt aggatttttc gattgaaagc
    11401 ttaaactttc aattcctgta gaaacaccag gaatctcaaa cttatctttg gctgaataag
    11461 gagccaatac tctgaagctt gctttcttca catctgaact tctgattcct ggaggagaat
    11521 caggtggcac tactttgaca aatgaaatat tcacattttg tctctcaggt atctccaact
    11581 tgccattagt ttcaggagtg agactaacat tcatagaaga actgttgaaa atatcaataa
    11641 tattattatc ctttgttacc atagaaacag caatcagttt tgcattttgt ccagtatatt
    11701 cttctgctgg taatccaaga cttttaataa tattcagata agttctatca tcaagaaatt
    11761 gcatatctac tggtaattca agcatttcac tcatagaatg tgaaccctca tagttcaaat
    11821 aatcgtctgt aagctcactt actttggcaa cacatgaata ggtcataagt ttctgataca
    11881 aaccttcgta aacaccattt gcagttttta gcttatcgta aagcttcaac atttcgttgt
    11941 ctttcatacc ttgtgtagaa aaaataatat cctgatcagt aacctctgac ctctgatttg
    12001 acatctgttt taggtctgct actaaagcac ttgtagatat aaataaaact atgcttaaaa
    12061 caagcgacaa tacaatactt cgataacgtt tcttgtttct tttaaagttt ttcatagcaa
    12121 gaactccctc aagaccataa actcgctgtg ttagttttga tgtcttcaca gatttagatt
    12181 caatttttac ctcatttgtc tgacgaatac actccatcac tggtgtgttg gcagcctttc
    12241 ttgctggtat gtaagcagaa attaagattg taaccatact taccactgct gcaacgatga
    12301 ttgcagggat agacaatgtt aatgtcaacg gaacgtttac atagagaata tttgcaaagt
    12361 ttttagctat gacagaaatc acaagtccaa tactgactat accgattgca acaccaattg
    12421 gtataccaat cgcaccaatg caaattcctt caaatagcac cgaatttcgt aactgttttg
    12481 ctgtagctcc aacagatgaa agtattccaa attgatgtgt tcgctcgttc aatgacatgt
    12541 taaatgaatt atatattaga aaaattgagc ctagcatgat tatggcaagc acaatgccac
    12601 caaccgagta caaaaacata ttgaacaaat tgttatcaga aacacctatg aagcgcaaca
    12661 cattgtcatt gaaaatggaa gcatggtctt tagtgctact tgtgtaagag cgaatctgat
    12721 gtggattttt caatgtgaca aaaaggctaa gactgtcagt cttgacttgt ttatcttctt
    12781 tggttatcaa ggtatatcct ggtgcagaag attcttcaaa tacgggcttt ctacagatac
    12841 ccacaactgt ataagttttc ttatcctgtg gcacaaaaac ttcactccca gcagtgtagg
    12901 ggtcactctg accaagcttt tcattttcac tcattcgttt ccctacatcc aaagaaatag
    12961 tatcacctac tgcaaatttg acaccaccat ctgtcgcaac tttacttgaa acaagaatct
    13021 ctctactatt ttcaggtaat cttccagaaa ttaaagttat aggcaaagta tcaaaagctt
    13081 ctttgctaaa tccagcaata aaaagatatg gtttatctgg atttctgtta ccttcaagtg
    13141 ttgcatagcc aatattttca aatgttgcag tatttgcaac ttcatcattg actctttgtt
    13201 cctgtacaaa agaagaatcc acatccaaaa actggacatg ccaatcacca tattttataa
    13261 tagctccatt tgtcatatag ttcatcaaag aaactccaaa ggtagtaact gctgtaatca
    13321 tagcagctga cagaacaact cctataattg ttacaagagt tcgagtgcga ttctttttca
    13381 atccttgcag agcaactttg ttaaaaatgt tcattaacga ttcaccttcc tctcatcacg
    13441 caccactttt ccatctgaaa tacagataat tctatctgcc tgtaaagcaa tgttttcatc
    13501 atgtgtgaca ataatgagag tttggtgata tttactatgg ctctctttca gtaactggat
    13561 aatctcttgt ccatttttac tgtctaagct acctgtaggc tcatcagcaa gcataactgc
    13621 tggtgcattc atcaatgcac gaccaatggc aacacgttgc tgttggcccc cagaaagttg
    13681 atttggtaaa tgtgtttttc gttcttttaa accaagtaat tctaacaagt catttaaccg
    13741 ttcttgattg accttacgct tatccataag tataggcaat gtgatatttt ccactacatt
    13801 taatgtgggt atcagattgt gaaactgata aatcaatcca acttgtcgcc tacgaaaaat
    13861 agcaagtttt tcattgcttt ggccatatac atcttgcccc tccagatata tctttccact
    13921 tgttggtacg tctactcctg caatggaatg tagtagtgta gatttaccag aaccagaaga
    13981 accaatgatt gcagtaaact cccctttttc aatagtaagt gagacatggt caagtgcagt
    14041 aaccttattt tcattcttac cataaacctt acataaatct tcaatcttta aaaacgccat
    14101 tatataagtc tcctttcata ttttataaat atatcataga actttcaact tacatttaag
    14161 tgacatttaa atgagagatt cgtcactttg gaaaacgaat aacaaatatt gcaccacctt
    14221 gtgggtgatt ttgtgcagta attgttcctc cttgtctcat aataatcgtc ttacaaagtg
    14281 ccaatcctat tccataacca gctgtgcttg aatttttccc acgataaaat ctgttaaata
    14341 gacatggtaa gtcttctttt ttaaagcctg ctccgttgtc atgaatagta agttgagtaa
    14401 acaaaaagtt gtcctcacaa acgatatcaa tttttccatt atcacccaca ctctccatgc
    14461 agtttttaaa tatattttga attgcttctg aaagccaatt taaatcaccc aaaatcctta
    14521 ctccttttgg tatatcaata tgtaaagtga tattgtgcag ttccatggga atcaaaagtt
    14581 gatgaagtgc agaactaatc aaattattca catcaatttg ttctttttga aagactacaa
    14641 tacctgcatc taaacgtgat aattttaaaa gggaagtaat cagccagtcc attcgtataa
    14701 gtaattcctc tgtttctcgt atcaactctt ttctctcatc ttcatcagaa gtattctcta
    14761 acagtgacaa aataagagtt actgatgtaa gaggagtgcg aagctggtgg gctatgtcag
    14821 ctagtgagtc agcaaggtgt tctttttctt ttttcagtgc gtaattttgc tctctaatac
    14881 gtaacgtcat tttagttatt tcactttgta gaatagaaag ctcaccctca tctgattcag
    14941 caatatacag atggttagca ttatgaagca caagattaat ttgttctgaa atctgtgcaa
    15001 tacttttgta tcgattttta gtaaacacaa aaaatgctat tccaaaggta gtggcagaaa
    15061 caatagaaag tattccagtc ataatactga ttgcaaatcc cagggctaca gtgagaatag
    15121 taattaataa gaataaaata gcaaattttc gaaactctct atttcgaaac ataacttccc
    15181 cccaatctat accctgtccc acgaacagtc aaaattattt gtggacttga agggtcatct
    15241 tctatcttct ctcgcaagcg tttgatgtac acagtcaatg tgttgtcatt aacaaactca
    15301 cctgccgcat cccacaattc atcaagtaat cttaccctcg taataatact tttaggattg
    15361 ctaataaaca ccaataacaa acggtattct aaagctgaaa gaaaaacctc attgccattc
    15421 tttttcacaa caccggttgc cgtatctaca taaagcccac aaatttcaaa agttgaccca
    15481 aaacgcccac tttttcgtag tgctgttcta attcgcgcaa tcagttcacg tggacgaaaa
    15541 ggtttagtaa tatagtcatc tgcacccata ttcagtccag taacaacact tgattcatca
    15601 ccagaagccg tcaaaaagat aacaggaatg tcttgctttt ctttgatttc tgtgcaaatc
    15661 gtaaacccat ttccatcagg taaagaaata tcaataagtg ccaaatcaaa tttattctta
    15721 gcaatctcta caagtgcttc acttcttgtg gatacatggg ttactataaa tccttctgaa
    15781 ttaagcaaaa gaataagatt tttggcaatt gctttatcat cctcaactaa aaatatccgt
    15841 ttcatttact ttcatcctcc tttattttat tagttttata tattccagca gagcaatagt
    15901 acactactat tttacttatc attacacaat tttcagtagt ttcttttgtt cttatataat
    15961 ccatgcataa tacctccatt gatttattat atttcttata acataaatac acaagataag
    16021 acaaaaaaca taatgtaagt gcaagttttt taactcacta acttataaac atacactcct
    16081 actaacacta ctttcttgat gacacatctt acaagcttat attcatacat caatgaaaaa
    16141 ttaaatttat ccatttatta aaataaaagg caatagcttc tatagctaat gccctttatc
    16201 ttaaaatgat ttaaatacta tacaattttt ataaatggct ataaataaac tcgtatagaa
    16261 tataaaattt atctacagtc ttttgttata caaaatttat aacctcttat agaacaaatt
    16321 taccactttg taaacttatc acttttactc agctatatta taaactttta atttatcttt
    16381 gtttaaatgt ttcttcttaa cttctactaa agcttttagc gtatccaaag atgtcattat
    16441 ttcagtagaa aattcaatgg ctgttcttct catcttaaat ccatctcttg tagagtcatt
    16501 tcctttagtt ggtgtattta ttactatatc aacttgttta tttcttatag catctaatat
    16561 gtttggtcta ggctcttcaa ttc
//
LOCUS       DAEPOZ010000065        16546 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.065, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000065 DAEPOZ010000000
VERSION     DAEPOZ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16546
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.065"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(134..2272)
                     /gene="vanT"
                     /locus_tag="KR440_001623"
     CDS             complement(134..2272)
                     /gene="vanT"
                     /locus_tag="KR440_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBH0698678.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            complement(2290..3096)
                     /locus_tag="KR440_001624"
     CDS             complement(2290..3096)
                     /locus_tag="KR440_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBH0698679.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            complement(3093..4193)
                     /gene="vanG"
                     /locus_tag="KR440_001625"
     CDS             complement(3093..4193)
                     /gene="vanG"
                     /locus_tag="KR440_001625"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HBH0698680.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            complement(4450..5532)
                     /locus_tag="KR440_001626"
     CDS             complement(4450..5532)
                     /locus_tag="KR440_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0698681.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            complement(5582..6283)
                     /gene="vanR"
                     /locus_tag="KR440_001627"
     CDS             complement(5582..6283)
                     /gene="vanR"
                     /locus_tag="KR440_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HBH0698682.1"
                     /translation="MNENILIVDDKKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KDLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            complement(6717..9248)
                     /locus_tag="KR440_001628"
     CDS             complement(6717..9248)
                     /locus_tag="KR440_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBH0698683.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVKEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            complement(9356..9973)
                     /locus_tag="KR440_001629"
     CDS             complement(9356..9973)
                     /locus_tag="KR440_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBH0698684.1"
                     /translation="MCKKTYLLVVSILIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIITPQQAMEIALKEQNG
                     MVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            complement(10335..11219)
                     /locus_tag="KR440_001630"
     CDS             complement(10335..11219)
                     /locus_tag="KR440_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBH0698685.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLVLSKRDIDLTLRRFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAVKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            complement(11410..12660)
                     /locus_tag="KR440_001631"
     CDS             complement(11410..12660)
                     /locus_tag="KR440_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0698686.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFIYIIVRDTPKEYGFNVDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDCKAI"
     gene            complement(12882..13646)
                     /locus_tag="KR440_001632"
     CDS             complement(12882..13646)
                     /locus_tag="KR440_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0698687.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            complement(13640..14365)
                     /locus_tag="KR440_001633"
     CDS             complement(13640..14365)
                     /locus_tag="KR440_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0698688.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            complement(14367..14735)
                     /locus_tag="KR440_001634"
     CDS             complement(14367..14735)
                     /locus_tag="KR440_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0698689.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            complement(14911..16455)
                     /locus_tag="KR440_001635"
     CDS             complement(14911..16455)
                     /locus_tag="KR440_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBH0698690.1"
                     /translation="MRNLQKKLMFFLFICIILQITYTTPVYSLCKLYKNNSINNFESI
                     VSNNQFISKYAIQIILIMAIICMMLIFYIIYDKLNFKIKLQKIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKSNIFEEEM
                     FARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPED
                     RDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIY
                     LQPKIDLNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIY
                     LRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDN
                     FDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSK
                     IIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIV
                     SNLVRG"
BASE COUNT         5652 a         2937 c         1654 g         6303 t
ORIGIN      
        1 gaaacatcac ctttttataa cttaaattgt catgtaagtt ataaaaaata tatattagac
       61 ataatattac taaaaatgtc tagtcagagt aaaataaatt acacagtaca acaaatatta
      121 ctatttaatt gagttacttc tttataacat ttaaccttct tcctactctg ctcaataatt
      181 cattcgatat actacatgag ttatttgcaa cttcagaagc agataattca gataaattat
      241 ctcttcctat tattattgcc gtattaccaa cttctacatt aggtatatct gtaatatcaa
      301 cagcaagttg gtccatgcaa attcgaccca ctattgctgc tctacaacca tttattatta
      361 cgtaactttt tccacaggat aaatttctag gtataccatc ggcatagcca atagataata
      421 tagcaattac gctatctctg cttgcaataa agactcttcc atatccaaaa ctatcatctt
      481 tttgtatttt tctaataaga attacttgtg attttaagga taaaacaggt cgtaaatcaa
      541 gttgcaattt agtcgtatca tttggagaac ttaatatccc gtataatgca attccaattc
      601 ttacataatt acacttaaga ttgggataat taaaaaaacc ataactactt tgaatatgta
      661 ctttaggaat ttttatacct ttttttaaca gaaaatcaat taacttataa aaacattcaa
      721 tttgtttatt tgtaaagtct acatcttcat ctttcagact atctgaaaca cacaagtgtg
      781 taaatatgcc acatatatct aagtatttta atttaaatgc ctttaaaatt gacctcacat
      841 cattaacatc aaaacccagt ctgtgcatac ctgtatcaat tttaatgtgt gattttatgt
      901 tgtatttctg tttgtttaaa gagatagcat gattaaggtc aataacactc tgtgtaagat
      961 tgtatttatg aagttccttt gcccttattg ggttagtata tccaagaatc aatatttctc
     1021 ctagtattcc atatcgacgt aaatcaatcc cctcatctat tgttgctact gcaaatgctt
     1081 ttacacctat ttggtttgca caagtcgcta cttcaaaagc actatgacca taggcattag
     1141 cttttacaac tgccatcaat tcacaatctt ctggcatagc tttttgtatc atctttacat
     1201 tatgttccaa attatttaaa tcaatttcaa tccaagctct attggtattt accttatgtt
     1261 ctcctttcaa gtcttttttt agaggtttaa gaatcaatat aataattata gaagctataa
     1321 aagatgctat acttaccact atataatgaa taaggctatt gtcaaccaag acagtttgta
     1381 ttccgaatat tttagaaaac atacgaataa caacaatcat cataggatgt ataatataaa
     1441 taattaatga tgatgttcgc aataacctag ctcgagaacc ttttaaaaat gttaaggctg
     1501 taaataagaa atacatacat ggcaataaca taatatacat gctatcatga cgctgtaccc
     1561 ctagtttatg taaaaacatt ccctcacata acataagtaa gaatgatata agaaatccaa
     1621 tcaagctatt tttagttgac atataattag atttattaga tattattcca ccaagtatga
     1681 aaaatacagg tgcaaaaaat ataccatttc gtgtataatc tgatacttca aaaatatacc
     1741 catataaatt ctttaatagt ggtatttttt ctaaaaaacc ataataacta tcaccaaaca
     1801 taccaattac ataaataaca atagtgatta ctaatgagta ctttaaacct agttttttta
     1861 caagaaagta tgcaatactt gcaccaataa ttgatgcagg tagataccat aaatgatata
     1921 aagtcccatc aaatacaatg tccttaataa tattgggcac taaattattc atggaaaaat
     1981 atccagtgta aatatttaag ggaatataaa ttacaatagc tatcccgtaa ataaataata
     2041 cattttttag aaaagatttc agtttattac aattataatt atatgttgaa attagaaaaa
     2101 agcctgatag cataaaaaag aatggcacag caatacgagc aattatacta gttaacacaa
     2161 agtcagccat tttactatat gataataatg gagatgtatg aattgcaatt ataagtaatg
     2221 cagctataat cctaaaataa tcaagccctg cataagattt attcctaatc attcttgttc
     2281 ctccatttct tagtcctttc tatttctcca aaatgtcaca ataaaaccat ccttattgtt
     2341 acccgatatt tgatatacac attgttcagg tatagagatt ctcacttctt ctttagtatg
     2401 aataggcaca taaaagattt ctataatttt ttctttatct tctattttta aacaatttgt
     2461 atacgaaaat ctcttaatat attctatata ttcttcaagg gcgagattca ttttattgat
     2521 aatctcagaa tgtggatatc cgacataacg aaaatgccat ggttcttgag atattcctgt
     2581 tatttcttct tttttacact gataacgctc tataaaacca taatgtagtg ctgccttacg
     2641 gaactcatta caaataccat tataaggaaa ttcagggcag ataaaatcta taacctcttt
     2701 cttcaaccct aaatcaatag caagccctgt ctgatgttca ctgtgatttg gaagtgctac
     2761 aaacttttta gtaaagtctt ttccattgtc acgcagagag cttgaatata tatcttgttg
     2821 ttcctctgca gtacgatatc cacttacagg tactatttca tttattccac atatactttt
     2881 taaaatattt tgtaaaatat ttgtagcttc acgtttcata agaatacttg gaaaccttgt
     2941 atctacagga attaaatcta tatcttcaga tgttttaact ggtaaaaaag catttactag
     3001 aattagattc ccatcataaa ctttatcttt agtcaataag atagtatttg tcattttatc
     3061 ttctttattt ttatctaata aaatattatt catcatttta tgtacacctc aattatctta
     3121 tccaatattt cttcaaaaga cagtccaata cctttcatca tattcggata acgactatga
     3181 gaagtaaagc caggaattgt attgacttca ttaaaaataa tttcaccaga tggagttaaa
     3241 aacatatcta ctctagcaaa tccagaacat ccaagagttc tataaataat ttttgctgtc
     3301 tcttgtattc ttttctcaca atccaaatca attctagctg gcatatgaat ttttgaactt
     3361 ttcaatgtat atttttctgt ataatcaaaa aatccttttg acaattctat ttcatctact
     3421 cttccaacaa gtaagtcatc gtttcctaaa actgcacacc ctacctcaaa accatcaata
     3481 ttttcctcaa ttattacttc attatcatgt tcaaaagcca agtctactgc tacttgtaac
     3541 tcttcttttt tataaatttt actaatacca aatgatgaac ctgcacgcac aggttttata
     3601 aataatggat aagttaagtc ttttgacagt tcagccacat taaatttatc tgagtatttt
     3661 agtaatattg attttggtac tttaatacct tctgaatgaa caagtttatg tgatttgtct
     3721 ttgtccatac ataatgctga agaaagagta ccacatccaa taacctgtat acctgccaat
     3781 tcaaataacc cctgcactgt tccatcttct ccattttttc catgcaatac aggaaataca
     3841 aaatcaagtt tagtgtattt aatatcattt tcacatgatt caatagcact atttggaata
     3901 tgattctgag aaacagcatc aggaattaaa aattcaataa taccattttt gacacggttc
     3961 tgcgaaacaa taactggaat tatatcagta tccctttcaa accaagtgtt attctggata
     4021 ttattataat ctccaatata atgataccaa ttaccctccc ttgtaattcc aattggtata
     4081 atatcatatt tttccgtatt caaattctca agtactgcaa aagctgattg taatgataca
     4141 tcatattctg ttgagtttcc accaaatata actgcaattt ttttattatt catctttgat
     4201 tctcctttgt tgatttattt atttttatac aatctacaga aacatgttaa aatacttttt
     4261 ctatttcaaa tccaatcaac cttcacttac aattgcatct gttccacata atatattttc
     4321 acatttttct attgatataa ttactgtatc aaaataaaag cactcttgtt ttgatacagt
     4381 aattatatca ataacaagag tgctctttat ttatcctaaa gaatttccta tttttttata
     4441 cttaaattct tacaaatttc ctacaatcgg aataataacc ttaaaggtaa ttatattgtt
     4501 ctcgctatga gctgttattg tcccgttatg aagctccact atttctttag ctatagcaag
     4561 tcccaaccca gctctaccac tgtttgaatt acgagatgta tccaacctgt aaaattgttc
     4621 aaaaatacgc tcaagttttt cctctggaat cgtattgcta tgatttataa atttaagctt
     4681 tatataatca tcctcctgta cagctgttat ttcaatagta gtatcttcaa aactataata
     4741 aacagcattt cgtaataagt tatcaaatac acgttgcatt ttattaacat cacaccttat
     4801 catcatattt ggtgtaacat tcagtgtata ttttaaattc ttactcatca aagtaggttt
     4861 aaactcatat gttagttgtt caagcatacg agttaagctt actgtactgt attcaagtga
     4921 tatatttgat aaattaaacc ttgtaatttc aaagaattca ttaattaaat cttcaagacg
     4981 ctctgcctta tccaacgaaa tagataaata tttttttcgc agttcttcag aaatctgact
     5041 ttcatctcga agtagtgtca gataccctat aactgatgta agtggtgttt ttaaatcatg
     5101 tgcaagataa acaataagtt cattcttacg ttgttctgcc atttgtgtct caaatttacg
     5161 ttgttcaagt atatgtttaa ttgagtttat tttcttttct attgccaata actctggaga
     5221 taaagccact tctccaacat cttctttgat aagagagtca ataccttgat ttatttcact
     5281 aaaatatttt gtaaaccaat ttaaataaac acggaatatt ataaaaaaca caatcaccat
     5341 agctagtatc atgaagagct ccatgctatt acggaaaatc tgctgatata aattcaatgc
     5401 agctttatat tccatacgaa aagcattttc aaagaatgat actatccaga tagaaaactt
     5461 accatacaat ataaaagaat ataaaatcca tatgaaaata atggctataa atgccattaa
     5521 agctgttctc ataaaaattt tatttctcac ttgagtaaag tcaggattga ctttaatatt
     5581 tttacttttc aattgtataa cctactcccc atacagtttt tataaatttc gggtttttag
     5641 aaggttcatg aattttttca cgtaatcgcc ctatatgagc cataactgtg ttattattat
     5701 caagatattt ttctccccat acagcttcaa acaattcttc agaaggaacc acttttcctt
     5761 gatgttcaca taaataccaa agaatagaaa attcaatcgg tgttaatgct acttctttcc
     5821 caaataaact acatttatgt gtttctttat tgataatcaa tcctttaata tcatactcat
     5881 taacaattat cgactgttgt tcataagaat tattatatcg catatatctt cttaattgtg
     5941 tctttactct tgcaacaact tccaaagggt taaatggctt tgttatataa tcatcagcac
     6001 ctatggttaa acccattatc ttgtctatat cttcaatttt agctgttaac ataatgatag
     6061 gataatagta tttttctcga attttctggc aaatatgaaa tccatcaata tcaggaagca
     6121 taatatctaa aatagccata tccaaatctt tagattcaat acattctaga gcttccttac
     6181 ctgtataaaa cttgtatact gtaaaacctt cattttttaa atatacttca attaaatctg
     6241 ctatttcttt tttatcatcc acaatcaaaa tattttcgtt catagattat ttttcactct
     6301 ccttattatc gaggtcatca tttataatat tggtaaattt acatatcaaa taattacaag
     6361 catatttgta actaattttt ataagaaaag aactactaca tcagaatgtg ttgatgctac
     6421 tattggaaca caaaaactga gattaattgt tattataaaa actcccctaa cacgtcctta
     6481 ttatcgcata acaggtatta actttcaata aaaatatcta tcagtcaaac acaattcaat
     6541 ttattatacc agagacaatc catataaaaa acaattaaac ctatgtatgt aaccacagac
     6601 attactaaaa tttccatgtt attttatata aaaattcaaa aaaggagttg aatttaaaat
     6661 ttgattagaa aaaaatcaat tttgagacaa ctccttttta ttttaatgta tttaaattat
     6721 agtatatcag ccttcataat atcctcaaaa tcaccttttt cagatattaa tcttatcaat
     6781 ttaacagtac agcttaaatc atttataagt atagctccaa ctaatgaacc atctttcata
     6841 aataattttt tatatattct ttgaccttca tcttttgatg aaatttcttt atcaaaattt
     6901 tgtatattac caatactaaa tacttttaca ttcattgcat ctaatgaaac tggatatact
     6961 tcactttcaa atgtcaaatt atctccactt gcatttgctc ctgctatttt tcccatttct
     7021 actgaagatg gccatattgc taaactaata tttcccactt gtgcaacatc tccacaagca
     7081 tatatatctt taacatttgt ctgtaatgtc ttatctacaa caatacctct atcaattttt
     7141 acagatgtat cctgaaccat ttgagtgttt gctcttacac caattgaaaa tactaccata
     7201 tcacagtcaa cactatctcc atctttaaat acaacttttt gagcttttcc atcaccttca
     7261 ataccatcaa ctgctacacc caatcttaca tctattcctg tatctcttac acattgttct
     7321 aatattttac taccctcttt atcaagttgt tttgatagta tgctatccat ggcttcaact
     7381 actactactt ctaatccttt tagtctaaat tcccatgcag cttcaagtcc caataaaccg
     7441 ccaccaatca ctacaacttt cttagatttt tctagtgctt cttttacact atataaatct
     7501 tttatatttc taagagtaaa cacattttct aaatcatggc ccttaatcgg tggtataaag
     7561 tttctacttc ctgtagctat tacaagctta tcaaatttaa tagctccatc attcactata
     7621 atagttttat tcacttcatc taatttatct acattcgttc ctaatattac tactatatta
     7681 tttttatcat accaatcttt atcttccatg tagaaatctg aaccaagttc ttcatttata
     7741 ccatcagaaa gtgcaggtct atagtaagtc aactcatctt cattagaaat catagttatc
     7801 ttacaagttt tatttctttt tcttatagca tctgctgcat aaaaaccagc tgctccattt
     7861 cctactatta cgaaatattc atcagtatct tttctaaaag taacttcatc ttcttcaact
     7921 tctataaatt ggtcttcctt agctccacat acagggcata cactaggagc actatcacct
     7981 tcaaaaactt cattacaaat tacacattta aatttcttag ttttcttttt acttccaaaa
     8041 gtagaattta acttatctag gaatttacct gtataagaat gtaatttatc aatctcttca
     8101 tttgatggtt taaagttaac agcaaaaggt tttactgctg tcattctaag ctgacttatt
     8161 ctctccatag cattttctat accttctcca ctccatccat atgaaccaaa tactgaagca
     8221 acttttccac catgaagaac tgggtttaac gatattaata tatcccatat aggctttaac
     8281 gcatctccta atattgttgg tgttccaaat aatacacctt gtgatactgc tatttttgcc
     8341 aaaatatctt cttgcttatg ctctattaca tcaaataaat ttactttata gttgctattt
     8401 ttttcaatat atgcttttat agcttctgcc ataatctttg tatatccatg tgcagaaaca
     8461 taacaaattg taacttcttt ttctaacttt atttgttcct ttacactcca gttataataa
     8521 aggtctataa tttttctagg attttcagtt aatactggac catgacctgg acatacagta
     8581 tctatatcta aatcttttat cttttctata gcagttacca tagatggttt aaatggaccc
     8641 attatacaat cataatagta tcttaaagca tccaagtaat ccttttcttc attttcatca
     8701 agtgtattta ctatcttatc attactatag tgactaccaa atgaatcaca tgttacaaga
     8761 gttttatcct cttttatata tgtgtatata gtatctggcc aatgaagcat aggtacagag
     8821 aaaaattcta aagttttgtc cccaattgat aatgtatctc catctgtaac tgcaacatat
     8881 tcaaagtctt tgtttacgat ttccttaaga tatttaattg ctgtttctga agctactact
     8941 tttgcatttt tagctaaatc caaaagcttt tcaacgcttc ctgcatggtc tggttcagta
     9001 tgacttacaa caatatagtc tattttttca aaatctatat ttaaatcatt taatctttca
     9061 atataattgt caaaatgctt atctttaact gtctcaaaca aaactgtttt ttctgttcct
     9121 tttaatacat atgaattata agtagttcca taaggtgtat acataattat gtcaaatact
     9181 cttaaatcag ggtcaagtga acctacccaa tataaatttt cttttatttt tagtgatttc
     9241 aatgacatac cgtactcctc ctaaaatagt tatttagtct ctttataccc tgcttgttat
     9301 attttaaaca aaatttattc aaaaaagaaa ttattttaaa gatagtttaa atcttctaat
     9361 cttctttttc taccttcaat acagttccct ttttagaatc tacttttatg tcatattcat
     9421 ttttatcttt atctattctt attttataaa aagtaacatc taaatctttg tctaaactcc
     9481 attctttaac cattccattt tgttctttta atgcaatttc catagcttgt tgaggtgtaa
     9541 ttatgtcatt taaatctaat ttttcttttc tagcaactcc atttaaatct tctgaatcta
     9601 acttttctgt tttatcttct aatacatctt ttgtattagc atcaattttc atcttatatt
     9661 cattattatc atctacacct tccacagtat aaacaaattt atttaaatct ctctcaagac
     9721 ttaaatctac tatatcagca tccttgtatt tatctttaaa tatgtttatt gcatctgtgt
     9781 atggcacttt aaaatcttgt tccattaaat tttcattatt tttttcatct tttaaagcat
     9841 tatttgaagc atttgcctcc ttttttgtat tatcttgtgc ttgtcctgaa tccttagttg
     9901 ggctcccact acatgcagtt aataaacctg ctataagtat acttaccact aataaatacg
     9961 tttttttaca cataaaaatc ctccttaaat atattaattt attctatatt atgccctcta
    10021 aaccctttat ttaccctttt atattaaaaa ttcctcctat tttatcaatt ttaaaacttt
    10081 atatttacta aaattaaagt tacataaaaa ttatactgtc tcaaaataga aacaagcatc
    10141 tttttcattg ttgaaataat aaaaatatat tttatttcgt acaaaaagag tgcctcatga
    10201 actaaaaagt tcattttgag acactcccat tcttttatat ttttacttac aaaataacag
    10261 tacttattaa ttttttacaa accttataag tatatctata ttatattctt aaatttaaac
    10321 actatattaa aaaattaaat tttgtttaat tcttttactg ttaaatccat tttaggatat
    10381 ggaatcgtaa ttccttcttc gtcaaaccta actttaacct cttctagtaa atcaagataa
    10441 attgtccaat aatcttctgt cttgcaccaa actctagttg caaatttaac agcattatct
    10501 ccatgttcta ctactcctat aaatggttct ggctcattta ttattaattt atgattatta
    10561 acaatatctt taagtactct tctaacatgt agtatatcat cttcataccc tacactaaaa
    10621 gtcaaatcaa ctcgtctagt attctttgct gaatagttta ttagactatc atttgataca
    10681 gaaccattag gtataagtat ctgtttatta tctggagtaa ccaattgtgt ataaaaaagt
    10741 cctatttgtt caaccgttcc tccatggcct gctgcttcta cgtaatctcc tactttaaaa
    10801 ggtctaatca atagtataat aaatcctcca gcaaaatttg atagactacc ttgtaaagcc
    10861 aaacctattg ctacccctgc tgatgctatc acagcagcaa aactggacaa actcattccc
    10921 caatattcca gaacaatgat tataacggca actttaagta cactcaaaag aagagacttc
    10981 aaaaaccttc ttaaagttaa atcaatatct cttttactaa gtactaacat aacatggttt
    11041 acaaattttt ttattatttt aaatccaata gaaagtataa gtatacctat tataagtttt
    11101 actccgcttg tagtagccca ttctagcaga ctttccatta tacctcccat atccatctta
    11161 ttaatgtcta aattatttaa atctaacttg cttatatcat ctattttcgt cgtatccata
    11221 aaaaactcct taccttatat tttagaacca atattatagt ttaaagttct tattttctat
    11281 ttaatataat acaataccat ttgtacatag taaacaagat ttatattcca attatacatc
    11341 ttaggaaaat gcaaactatt tgttatatat atgcaaaata taatactaaa ttaaactatt
    11401 aataaaacac tatatagcct tacaatcatc attttttacc cctatatgag atgatactat
    11461 agttataata cttagtacta taaatactac aaaacctttt ttatatccat caactatatt
    11521 atatgaattt acttgtaaag agatagctat aatcaaatta ataattccac ttcctataaa
    11581 ttcaaaagta ttaataacac tagttgccag tccagaataa atttctttat ttttatactt
    11641 ggcatcatta aaaacctgta gatgtgacat aactatacat cccataataa agaatgatat
    11701 tggtaaaact ataattggtg gttttacctg gtatacaact acaattacaa tccatataaa
    11761 caaatctatc attgctccaa attttattat attaaatttg ctagacctta ttttagcaaa
    11821 tacaaaatcc ataattattg aaccaaatat aaatccatat gtaaaaaatg atactataaa
    11881 cgcagaaaaa ctcttactca caccataaac gtccattatg tatcttactc cccacaagct
    11941 tataaatgca cttgtaagac caacaaaaga gaacattatc attgaattat accatgttga
    12001 tttgttcttt ataacagact taataccatc aacaatatta accttctctg acttttcata
    12061 gggttcagta tctacattga atccatactc ttttggtgta tctctaacaa taatatatat
    12121 aaaacatcca attacaacac caattatacc gattaataaa aatgaatttc tccaaccaac
    12181 aagttcagac aaaaacacaa gtggaaaagt agcaagaact ccacctaaat ttcctacaaa
    12241 tgaaaattta gctgttgcag aagcaaattc tgacttatta aaccatctcc cttgaatttt
    12301 taaaatacaa agtaatatta ctgatgttcc tgcccctaca attacacgag aaatatatgc
    12361 taattctata ctttgaatca aaccaaataa aattgaccct aaacttgcca ttattattcc
    12421 aagactgcta atttttcttg ctccatattt atcaatcaaa attcctgcag gtatttgcat
    12481 aaatgcatat gcatataggc aaaaggatgc tatatttgaa atttgtatag aattaaaacc
    12541 taactcattt gctaaattgt ctgaaacaac agcagttgac atccttaaaa ataatactaa
    12601 aacaaatgaa aatgtaatta taccccaaac actccactta gcctttgtat aacttttcat
    12661 atcttctccc tcaaatccat atatttttaa attttcttga tataacttat ataaagtgta
    12721 tagttattct tacaaatttt tacacgcaat atcattatac aacatatgta tatatttaac
    12781 tagaaataaa atactattca tattatatac atgaattaac tacataaata ataagagcca
    12841 tcttttaaca ttactcaaaa gatggctcca tataaaaaca tttattctaa atttaatttt
    12901 ttatctaata taaattttgt agcgaaaaat aacgctattg caactacaag actaccaagt
    12961 attgcaagca taaaactagg actctgataa agatgatata ccatatttgt atcttcattt
    13021 ggtaagatgc ttccaactgc atctccaaca attgatatta ctatatttat agctataaaa
    13081 gaagcaaatg ccactatgtt tctatgttta ttgaattttg gtaattgtcc cattgagatt
    13141 gatgtatata ataacaaaat aaatgatgaa tatgaaataa acattactac tactaataat
    13201 aaaatatcta aaaagttttc agaaatccat ttaaaactag tgttaaacaa atctaaaatt
    13261 tcaggtagta aaattccaga agtaccaaaa agcataagaa ctccaaaagt gaatactgca
    13321 actataaaac taagtacagc gtatataagt gctgttatac atttagataa tataagtgaa
    13381 ctagtgctaa caggaagagt aaacattaaa taaccttcat cccccaaaag attttttcta
    13441 aacctttgaa ttgtaactac aatagttaaa actcctaatg ccataaaaag acttgttaat
    13501 actagtattc ctattccctg tacctgaagt atatttgtat tcataaatat tccattaaat
    13561 acagctacta tcaatatggc tatatataac ggtataaata ttcttccact tgcttttaac
    13621 tcatatttta ataatttacc taacatttaa attcctccct aaataatgca tctatagatt
    13681 ttcctttttc ttctcttatt gcttcaacgt ctccttgtaa cactatttct ccttttgata
    13741 tgaatattat ctcatcacat atgttttcta tttcacttat taagtgggta gcaatcagta
    13801 aagtactatc ttcagaataa ttttttaata tagtcttaag tatatatgac cttgcagctg
    13861 ggtcaacacc acctataggt tcatccaata tatatatgct cgcatttctt gacattacta
    13921 aaataagttg aactttttct ttagttcctt tagacattgt ctttagcttt tcatttacat
    13981 caatatctaa gcttttaatc atctcatttg ctcttctaac atcaaaatca ctataaaaat
    14041 catagaaaaa cttaagtaaa tctgatacct tcatccaatc atttaaataa gttctttctg
    14101 gtaaatatga aactattttt ttagtatcta tggatggttt cattccattt atcataattt
    14161 caccattatc aggctgtaat aatccattca ttagcttaat cattgtactt ttaccacttc
    14221 cattaggccc taacagcccg actatttttc cctttggtat atttaaatca atatttttta
    14281 aaacattctt agttccataa ctcttgttta cacctttaaa ttcaactatt ccatcttgag
    14341 aaacattgct tatattttta tccattttat aattctcctt tcagattttc tgtaataagt
    14401 tttagcattt catctctatc atatccaagc ttttctaagg ttacttttaa tgtatttatc
    14461 tccacattag ctatttcctc tttcatagct tttatttttt ccttatcatc tgttacaaat
    14521 cttcctttag ttctctgact ataaactaaa ccttgtcttt caagttctgt tagagccttt
    14581 tgcattgtat ttggattaac ttccgcatct tctgctagtg ctctcactgt ttctaattta
    14641 gatcctattt taatttcccc tgaaattatt tttaacttta aatgttctac taattgtata
    14701 tatataggtt tgttattatc aagttcccat tccatgttca tgcctcctta ttatattatt
    14761 gtattaattt attaatacaa taatataata atacaaagta tatgtcaatt actttttagt
    14821 aaaagtataa aaaatttcac aaaaaaaatg aacactacat tagagtccat ttttaacaca
    14881 atttattaaa ttctttatat tacagattaa ttaccctctt accaagttgg atacaattaa
    14941 atgttcaaat tcttcaattg gcattggctt tccaaacaaa tatccttgtg ccatatcaca
    15001 ctttacttct tttaaaagtt taaactgttc ttctgtctca actccttctg atatgacttt
    15061 catacccaac tcttttgcca tttctattac tttagtaaca attattttgc ttcttttact
    15121 attataagtt tccataataa atttttggtc aagttttaag atatccatag gaatttcttt
    15181 taacatattt aatgatgagt accctgaacc aaaatcatcc atagatatta aaaatcctat
    15241 tttttttaaa ttattcatta tatcaattaa aatatcaaaa ttatcaaata tgatactctc
    15301 cgttaattca agttctatat actttgcagg tatattgtat ttatcaatta tatttttgta
    15361 tgtttctata aaattattgc aatataaatg tattcttgaa acattaactg aaacagtaac
    15421 tggatttatt cctgcatcaa tccattttct aaggtatata catacttctt caagcacata
    15481 taaatctaaa tttataacaa aaccgtttct ttcaaaaaat gggataaatt catttggagg
    15541 tattagccct ttatcaggac gtttccacct aacaagtgct tctgaacctt gtatctctcc
    15601 tgtattaaga tctatcttag gttgtaagta tataataaac tctttatttt ctaaacttga
    15661 aaacattgta ttttccatct ctttttcttt tatctcttgg tcatgaatct tattatcata
    15721 aaatgcatat gaacttttat gtcctccctt tgccatttta tgaggtatat tagccctatc
    15781 tatgattgag ttgatatctc tatcttctgg aagtataaaa tagattccac aacttaaaac
    15841 taacttatac ttttcttctt gattattatt aaacaagtct aattcctcaa atatagtttt
    15901 taacctcttt gttatatctt cttcttctat atatttcata attataataa aattatccgc
    15961 agatattctt gcaaacattt cctcttcaaa tatattactc ttaataattt ttgttaaatt
    16021 acgcaaaatt tcattcccaa cttcatatcc aaataaatca tttatatatt taaatttatc
    16081 aatatctata tataataaag catactttac ttgagtattt ttacatagta ttttgttagc
    16141 atcaattaca aatttatcta tagtatttgc tcctgtaaga ttatctgtat atgctatctt
    16201 ttgcaattta attttaaaat ttaatttgtc atatattata tagaatataa gcatcataca
    16261 tattatagcc ataataagta ttatttgtat tgcatatttg ctaataaact gattattaga
    16321 cacaatagac tcaaagttat ttatggagtt gtttttatag agtttgcata aagaatagac
    16381 tggtgttgtg tatgtaattt gtaaaattat acaaataaat aaaaaaaaca tcaatttttt
    16441 ttgtaaattc ctcataaaaa tttcctccca ttaaaacacc tctattttat tagcaaataa
    16501 gtacatctca aaagtttcca tttcttgtaa aatggatagt ttatat
//
LOCUS       DAEPOZ010000066        16509 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.066, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000066 DAEPOZ010000000
VERSION     DAEPOZ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16509
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.066"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..1405
                     /locus_tag="KR440_001636"
     CDS             <1..1405
                     /locus_tag="KR440_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0698691.1"
                     /translation="DLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITE
                     LEYNLGYTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVTTL
                     IDKIENRYRVKITKDEIMYIYIFLVSSGFSSENNSSYNDESDILSESEKVSSIMIENM
                     SQFLNINLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTI
                     NIMTEYDNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKRE
                     FPEWTIVDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMESDINNIKNAL
                     LSKPKKSMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIH
                     KQSKLNKGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKE
                     DIKILFKNNLLEKTNGN"
     gene            1395..1844
                     /locus_tag="KR440_001637"
     CDS             1395..1844
                     /locus_tag="KR440_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0698692.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNISERLLNVHTKKEILDIFYEV"
     gene            1944..2831
                     /locus_tag="KR440_001638"
     CDS             1944..2831
                     /locus_tag="KR440_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH0698693.1"
                     /translation="MAIYLEMPKLIHEFPFRSLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGGASK"
     gene            3236..4684
                     /locus_tag="KR440_001639"
     CDS             3236..4684
                     /locus_tag="KR440_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBH0698694.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKD
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIIHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MNYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            4710..7100
                     /locus_tag="KR440_001640"
     CDS             4710..7100
                     /locus_tag="KR440_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBH0698695.1"
                     /translation="MSLIFTTKDQLIEQGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDCFD
                     LSKGITSEHTRILDLNEGIVKRSFIWENSRGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVDDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGIRTYFEATNDEEFMMQNGLEILIETARFWVEFGDYIELKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKDWI
                     NAAQNIYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLNYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKKDKLSFEPRIPKHWKNI
                     KFNILFRDNLVSVNIEEYKSYYKLIKGKNISILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            7102..7773
                     /gene="pgmB"
                     /locus_tag="KR440_001641"
     CDS             7102..7773
                     /gene="pgmB"
                     /locus_tag="KR440_001641"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBH0698696.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     ISREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGIKDEFDCIVNPSLLERGKPYPDIFIEGSKMLNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLD"
     gene            7812..9212
                     /locus_tag="KR440_001642"
     CDS             7812..9212
                     /locus_tag="KR440_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH0698697.1"
                     /translation="MKNKYEEVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNENKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQGVIYAASV
                     IPALLTVWMMSYIEKFINKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVKESTDNVVEEV"
     gene            9529..10371
                     /locus_tag="KR440_001643"
     CDS             9529..10371
                     /locus_tag="KR440_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0698698.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            10516..12444
                     /locus_tag="KR440_001644"
     CDS             10516..12444
                     /locus_tag="KR440_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0698699.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKFVPDLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMTIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVASPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVDAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            12505..13932
                     /gene="ascB"
                     /locus_tag="KR440_001645"
     CDS             12505..13932
                     /gene="ascB"
                     /locus_tag="KR440_001645"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBH0698700.1"
                     /translation="MSFKDDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYRGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLVGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDDYR
                     INYLREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            14343..15119
                     /locus_tag="KR440_001646"
     CDS             14343..15119
                     /locus_tag="KR440_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH0698701.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IVKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            15135..15500
                     /locus_tag="KR440_001647"
     CDS             15135..15500
                     /locus_tag="KR440_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBH0698702.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWKSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(15666..16415)
                     /locus_tag="KR440_001648"
     CDS             complement(15666..16415)
                     /locus_tag="KR440_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBH0698703.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELISA
                     GKPFIFVCPTYAWRLPIVVTDFIKETKFLGDNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFETITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNS
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
BASE COUNT         6560 a         1736 c         2886 g         5327 t
ORIGIN      
        1 tgatttatta gatgaaaatt atgaacaaca tgatatatct gagttaacaa gattagattc
       61 tgctacattt agtgcattga taaatttatt tgatattgat tctattatat ttgtagaaac
      121 tataattaca gaattagaat acaatttagg atatactata aatcagccct actatataaa
      181 tttaattacc catatactta tatgtttaaa gagaatcgaa gaaggtaatc aaatagaatc
      241 tcaggaagaa agagaaattt ctgtagataa tttggacgag ttagtatata aaaatgtcac
      301 tacacttata gataaaatag aaaatagata cagagtaaaa ataacgaaag atgaaattat
      361 gtacatatat atttttttgg tatcatctgg atttagtagc gaaaataata gtagttataa
      421 tgatgaatca gatatattaa gtgaaagtga aaaagtttca tctataatga tagagaatat
      481 gtcacaattt ttaaatataa atctcaaaaa tgatgactta ctacaaaaaa gtctagcatc
      541 tcatgtaagg cctatgttaa acagattaag atatgatatc caaataaaaa acccgttgtt
      601 aggagagata caagagcgat tttcagatgt gctgggatta tgtatgatga ctataaatat
      661 tatgacagag tatgataatc tgaagaatat tagtatagat gaagtttcgt atcttgccac
      721 atattttcaa gcggcaatag aaagaaatat gagtagtaaa agagtaatag tagtttgtca
      781 tacaggatat ggaacatctc agctccttgc agccagatta aaaagagaat ttcctgaatg
      841 gactatagtt gatatagttc ctatgcatca gttgagtaag cgaaatttag atgatgtaga
      901 ctttgtaata tctactgtaa acttagattt gaaagataaa ttacatatag ttgtatcagt
      961 actattaatg gaaagtgata ttaataatat aaaaaatgct ttactttcta aacctaaaaa
     1021 aagtatgaac ttagatacaa acttattagg aatgtattta gaagataata tatattttaa
     1081 ttttgatgta gctcagatgg aaagtttaat aggtgtgaat ttagggagta aatatgaatc
     1141 tatttcatta ggaaaagatt tgaatatata tatacataag caaagtaaat taaataaagg
     1201 gataatgaat atcaataatg ttactagaac gattgatatt tatttagaaa ttagtaatca
     1261 aagtttttta aagaatattt taatggaaat atcaaattta tataggcaaa agaactatat
     1321 aaactatata atagattgca agagcaaaga ggatattaaa atattattta agaataatct
     1381 attggagaaa acaaatggaa attagagaaa tattaacaga agaaagcatt attttagatt
     1441 tgaaatcaac tacaaaagaa ggcataatac aggaattagt agataaatta tacgatacag
     1501 gaagtataac tttaaaaagt aagtttataa aggcattaaa acatagagaa aaaataggct
     1561 ctactagtat aggttatcaa atggccatgc cacatggaaa aacaaaatat gtaaagaagg
     1621 catccatagt aataggtaaa tctaaaaaag gtgtagattg gccaagtata gatggcttac
     1681 ctgtaaaggt aataattatg tttgctatgc cacaatctgg aggcaatgaa gatatgatat
     1741 taatatctaa aatagcttac attttaggtg gtaaaaatat aagtgaaaga cttttaaatg
     1801 tacatacaaa aaaagaaata ttagatatat tttatgaagt gtaggtattg tataaattat
     1861 aaatttgagg tttttaaaat aaaatgtgta ttcttttaaa tagtatagat taatttttag
     1921 acactagaga ggagaggata tctatggcaa tatatttaga aatgcctaaa ttaatacatg
     1981 aatttccttt tcgtagtcta tcaaatgaag gagaagtatt aacaacacca cactggcata
     2041 aggaaataga aatattactt ataacagaag gtgtagtaaa tctatttata aatgacaagc
     2101 ctatgcaatt aaaaaaaggc gagatagtga taataaaagg aggagatatt cattatatac
     2161 tgccatcacc gggaagtgaa agattagtat ttcaatttga tatatctttt tttaatgatt
     2221 taattttatt aaatgaggaa aaagaaagct taattaattt attcagtagt ataaaaaaat
     2281 gtagtagaga ttggtcagca aaaataagaa agtcagtttt cgatatattg atagatatct
     2341 acaatgaaga cctatacaaa atagaaggat atagttatgc aataaaaggt aaaatgtata
     2401 atttaattcc tatattatat agacagattc ctagagaaat tgaagaaaat acagtagaat
     2461 atgaaataaa ctctaaagag atattagaga gattgaacaa tatattcaaa tatgtagaaa
     2521 aaaattataa acaacctata aaattagaag atgtgtctta tgaagtagga tttagtgtat
     2581 attattttac taaatttttc aaaaaaaata caggaaaaac atttataact tttttaaatg
     2641 aatatagatt agaaaaagca aagtggatat tacttaataa caatatatcc attgtagatt
     2701 tggttgaaga agttggtttt ggaagtacaa aaacttttta cagagtattt aaagaaaaaa
     2761 ttggagtctc tccattagaa tttaaaaaaa gatataatag cttggagaaa aaagggggtg
     2821 cctcaaaatg aacttagttc atgaggcact ttcttttata tgaaatcaaa tatattttca
     2881 gtatttcaac aatgaaaatt ccattcattt ataaagtcta aattttagtt ctcaatacaa
     2941 tctctatttc gaaataaatc cttttatata tataaatata cagcaatata tgagaataag
     3001 ataacaataa tgaggatata aaaatgtatt tgctatatat ataattatat acatagatat
     3061 ctaatattga tatttaatat gaaagtattg aaaatagaca atattaatta tagtgagtaa
     3121 aaaatataat tggattttta aattgaatta tttaaaaggt atagtatgaa aaaaatctta
     3181 taactaaata tgagatagaa attataaaac tttatactgt gaaagaggta gatgcatgaa
     3241 attagggaaa gattttttat ggggaggggc aatttcagcc aatcaaagtg aaggatttta
     3301 tttagatgga ggaaaaggtt tatctcagtt tgataaactc ccattaaatg ataagagatt
     3361 aaaagatgta tgtttagatg aggataatat tttaaatgaa aataatcaat actatccaag
     3421 tcatataggg attaaatttt gtgaaaatta caaagaagat atagcaatgt tagcagaaat
     3481 aggttttaaa tgttttagat tttctatatc ttggtcaaga atattcccaa caggagaaga
     3541 aagtgaacca aatgaaaagg gattacaact ttatgataat ataataaaag aattaaaaaa
     3601 atataatata gagccattaa taacgataag ccactttgat ataccttatt ctctagttga
     3661 aaaatattct gggtgggctg ataggagagt ggttaattta tatgtaaaat atgctgaaat
     3721 tttgatgaaa agatataaag atgatgtcaa atactggatt ccttttaacg aaatgaatat
     3781 gataattcat attccatata taggtgctgg gttaactttt atagctgatg aaaatcaact
     3841 tcagaaaaaa tatcaagcag cacaccatca attattagct aatgcaaaaa ctatagaaat
     3901 aggaaggtat ataaataaag aatttaagtt tgggtgtatg ttagctgctg gaaaaacata
     3961 tccatacaca tgtaaaccag aagatgtatt tgcaacttta gagcatgaaa ggcataattt
     4021 gtttttttct gatgttcaag ttaggggtgt gtatccaagc tttatgaatt attatttaaa
     4081 atcaaatcaa ataaaattaa taataaaaga ggaagattta tcaacactta aggataatac
     4141 tgtagatttt gtatctttta gctattattc tagtgcttgt agttcagcta gagctgaagg
     4201 tttagaaaaa tcaaaggcaa acggaccaga aaccataaaa aatccatatc ttccaaaatc
     4261 aaatagtgta tggcaaaacg accctgtagg gttaagaata actatgaatc agttatatga
     4321 aaggtatcag ttacctttat ttatagtaga aaatggactg ggtactcaag atattgtaaa
     4381 tgaagatgga aaaataattg atgattatcg tatagattac atgaaagaac atattaaaaa
     4441 tatgattctt ggagtaaatg aagatggaat agagcttata ggatatctta tgtggggatg
     4501 tatagatgta accagtgtaa gtgaaggaaa gatgacaaaa cgatatggca taattcatgt
     4561 tgattctgat gactatggaa gaggctcatt taaaagaact aaaaaacaaa gtgctttttg
     4621 gtataaaaaa gtaatagaaa cagatggaga aattttattt aatgatatgg aaactcatgc
     4681 ataattcatt ttaagaaagg agtgttatta tgagtttaat atttactact aaggaccaac
     4741 ttatagagca agggtttagt aaggatacaa taaaatccaa tgaaacgttg tttacactag
     4801 ctaatggaca tttaggagtt agagggaata ttgaagagtc cgattttaat aatgagtatg
     4861 tagataatat gggtacatat gtaaatggat tttatgaaga atctccaatt acatatggag
     4921 aaagcgctta tggctatgca aaacaaaatc agactatctg taagttacct aatgctcata
     4981 tagtaaattt ctctattgaa ggagattgct ttgatttatc aaaaggtatt acaagtgaac
     5041 atactaggat actagattta aatgaaggaa ttgttaaacg ttcatttata tgggaaaact
     5101 ctcgtggtag aaaagttaaa gtatctatag aaagattagt atcacaagat atagaagaat
     5161 taatgatgat ttcatataca ataacaccat tgaattttaa tggaaaaata aaatttaaaa
     5221 ataaaatgga aaatagtatg gagaagttta attgtgaaaa agtagatgac ccaagagtgg
     5281 catctataaa taagaagcaa tttaaaatta atgtaaaaaa atataacaat aagtatttta
     5341 tgaatttagt tacagaaaga agtagattag ggttatggtg tgggtttgat tcatatatta
     5401 cttgtggaaa tattacaaat gagacctata atgtttcaga tgaatgtatt gagcgagaaa
     5461 tagaagtcta tgccaaaaaa gatgaaaggt taaatttaga aattgttgta ggctatagta
     5521 gagtatattc tgatttaaat ctaaatgaag aacgtttaaa atcattagaa tgtctggagt
     5581 cgttaaaatg tttagaatca gaagcacatt taaaatcatt agaattggtt ttaagagaag
     5641 catgtaatat tggatttgat aaagtcaaaa atattcagag aaagaaaatg aatgagtttt
     5701 ggacaacctc tgatgtagag attgaaggcg ataaaactct tcaactgggt ataaaaacca
     5761 atttatttca tatttatcaa tcagcaggaa gagatggaaa gactaatata tcagcaaaag
     5821 gtcttacagg tgatggctat gaggggcatt atttctggga tacagaaatg tatatattac
     5881 cattttttat atatactcaa ccatccattg ctagagcttt attagattat agatactcaa
     5941 tcttagatgc atcaagagaa agagctaggg aacttggaat aaataaaggt tgcttatatt
     6001 catggagaac tataaatggt aatgaatgca gtgcatatta tccagcagga acagcacagt
     6061 ttcatataaa tgcagatata gcttatggta ttagaacgta ttttgaagca actaatgatg
     6121 aagaatttat gatgcaaaat ggcttagaaa ttcttataga aacagcaaga ttttgggttg
     6181 aatttggaga ttatatagaa ttaaaaaata ataaattctg tataaatgga gtaacaggac
     6241 cagatgaata tacagcaata gtaaacaata attactacac taatttaatg gctaaatata
     6301 atttaatata tgcagtacag atggtagata aatttaagca taatttattt tcaaatgaag
     6361 tatttagaaa agttaaatta caagaatatg aagtaaaaga ctggataaat gccgctcaaa
     6421 atatttattt accatatgat gaagagaaga aaataagcaa acaagacgat tcatttttag
     6481 aaaaatcagt atgggatttt aaaaatacac caaaagaaaa atatccatta cttctcaatt
     6541 atcatccatt aacaatatat agatatcaag taaataaaca agcagatact gtactttcag
     6601 cgttgctttt ccctgatgag tttagcttag aacaaaagaa gagagatttt gaatactatg
     6661 aaaaaataac tactcatgat tcatctttgt caaggtcaat atttggaatg atggcaagtg
     6721 aaattggaaa tcatgaaaaa gcttataact attttatgga cacagcatta atggatatta
     6781 ataacctaca aaaaaataca agtgatggaa tacatgctgc taatatgggt ggaacatgga
     6841 tgagtttagt atatggattt gcaggaatga aaataaaaaa ggataaatta agttttgaac
     6901 caaggatacc taaacactgg aaaaatatta aatttaatat attatttaga gataatttag
     6961 tttctgtaaa tatagaagaa tataaatctt attataagtt aatcaaaggt aaaaacataa
     7021 gcatattaca tgataataaa gaaataaaat tatcaaagaa cataatagaa gttaataatt
     7081 gttttaaagg gagtaattaa tatgaaagca gtcttatttg atcttgatgg agttataaca
     7141 gatactgcta agtatcattt tgaagcttgg catgagatag caaaatcgct agatatagaa
     7201 atagatgaaa cttttaatga aaaattaaaa ggcataagca gggaagaatc attagataaa
     7261 atattgttat ctgctaataa agtagatagt tatactaaag aggaaaagtt aaaggtgtgt
     7321 gaagaaaaaa ataacattta tataaagtta ataaactcat taacatataa agatatatta
     7381 ccagggataa atgagcttat aaaagaactt aaaaaaaata atgtgaaatt gggaatagca
     7441 tctgctagta agaatgcacc taggatatta gaatctttgg gaataaaaga tgaatttgat
     7501 tgtatagtaa atccatcctt attagaaagg ggaaaaccat atcctgatat ttttattgaa
     7561 gggagcaaga tgctaaattt aaaacaatat gattgtgtag gaatagaaga ttcttattca
     7621 ggtattaaag ctataaatgc tgccaatatg acatcaattg gtgtggggaa aaaagaagta
     7681 ctaggaaaat gtgaccatat tgtagaagat actagcttat taaattacaa tacactatta
     7741 caagcttgga gtaaaaagat taatttggat taaaattata ttaaaaatta ttttaaaaga
     7801 gaggataagg tatgaagaat aaatatgaag aagtagctca attgataatt gatggacttg
     7861 gaggaaagga taatataatt gacttaactc attgtatgac acgattaagg ttcatcttaa
     7921 aagatgaaaa gaaagtcaat gcagataaat taaaatctat agataaggta gttggagtaa
     7981 actcaacttc aactcaatat caggtcataa taggtaatga ggtaggtgtt gtatttaatg
     8041 caataataaa taaggggata aataccacag gagatattaa aagcgatata aaaaatgaaa
     8101 ataaaaaaaa tagtatttta aataatataa tagatacaat aactggttgt atgacaccta
     8161 tgataccagc ccttacagca tctgggatga taaaagttat attatctctt tgtaccactt
     8221 ttaattggct atcatcaaat tcaagtacat atagagtatt agatataata ggagatgtag
     8281 cattttactt tatgcctata atacttgcaa taaatgcagc taaaaaattt aatgttaata
     8341 catcaatagc agttatagta gtaggtgtgt ttttacatcc aaatttctct gcatgggtat
     8401 caagtgggga ccctatatca tttataggag taccaattca aggtgttata tatgcagcat
     8461 ctgttatccc tgcactttta acagtatgga tgatgtcata tatagaaaaa ttcattaata
     8521 aattaacacc tagcatgtta aaaacaattt taaatccaac tttagtatta ttaataagtg
     8581 cgccaatagc attaatagta attggaccaa taggaaattt cttaggagag ggattagcta
     8641 gtataataaa tttactacaa ggaaggttag gatttataat ggtttgttta ttagcagctg
     8701 caatgccatt tatcgttatg acagggatgc atcatgcatt gactcctata ttcatatcag
     8761 catttgcaac tacaggacaa gaatcgctaa tattaatatc tcaagtttgt gctaatttag
     8821 cgcaaggtgg cgcgacatta gctgtagcat taagaagcaa agatagtaag atgaaacagt
     8881 tagcatcagc tgcatggata tcagctatca tgggtataac tgaaccagct ctttatggag
     8941 ttacattgaa acttaaaaaa cctgctatgt tagcatctat aggagcagga atcgcagggt
     9001 tatttgcagg aattactcat gtgactttat atgttccaca aaatagtata atggcagtac
     9061 ttggattatc aggggaaaag ggaatttcaa atatagtaaa tggacttata gttatattta
     9121 tagcattggt tgctccattt attatgacat atgtttttgg agttgaaaaa aatgttaagg
     9181 aaagtacaga taatgtagta gaggaagtat aatattaact atttacatta atagaatttt
     9241 tattattatt gactataact tgttttgtat ttatattgat gcaaagaaat aagttaatat
     9301 taatgataat aattattata actgacaaaa agcaacacaa aagattgatg aagtatatgg
     9361 aaataaaaag ttgacaaaat atgtctgatg ttttatcata ttaattggaa ttaggtagta
     9421 attgtttaag tacaagctaa gcttattcat taataatagc agtatcctag gaaggagtat
     9481 gcccattatt atggataagc tttttttatg cttaaaagga ggataatcat gataattaat
     9541 aagatattaa acaataatgt tgtaatcaca ttagatgata atgatgaaga agttatcgtt
     9601 atgggtaaag gaattggtta tcaaaaatca aaaggcaatc taatagataa aactaaagta
     9661 aataaagtat ttagaatatc aaataaagaa atatcaaaca aactacaaga attactcaat
     9721 aatattccta tagaacacat gaaattatca agtgaaatta tagaatacgc acaaataaag
     9781 ttaaacaaga agctaaatga aagtatttac atatccttaa gtgaccatac atattctgca
     9841 attcaaagaa tgaaagaagg aataaatgta aaaaatgcaa ttctttggga aattaaaaga
     9901 ttttataaag aagagtttga aattggaatg aaagcactag atattattga aaacaaaaca
     9961 ggtataaaat taccagaaga tgaggctgga tttatagcct ttcatatagt gaatgctcag
    10021 ttaagtgaag gtcatacttt ggcatctgat attacgaagt taatacaaga agtacttagc
    10081 atagttaggt atcattttag gatagaattt catgaagaat cagtgtttta ttatagattt
    10141 ataatgcatt taaagttttt tgcgcaaaga ttgcttttag atagtgcaca tgaaggtgag
    10201 acagacaaag aattactaag cattattaaa tctaaatata ataaagagtt tgaatgtgta
    10261 gttaaaatta aaaatttcat taaaaaacaa tataactaca tattaacaga tgatgagctt
    10321 atatatttaa caatacattt agctaaagta gttaaagact caaatatgta agtgaaataa
    10381 tttttcagta gtatattaag aatgattgaa aggagtgata ctaacaaaat tatacctatg
    10441 gttggatggt aacattgagt tggccaaacc ttcaaatttc aaaaaattaa tttaaacgaa
    10501 atatggagga gaaaaatggg aaaatacaaa caattagcac aagagatagt aaacaatgtt
    10561 ggtggaaaag aaaatattag tggattagta cactgtatta caagattacg ttttacttta
    10621 aaagatgaaa gtatagcaaa tgacaatgta ttaaaaaaca tggaaggtat tgttactgta
    10681 atgaaaagtg gaggacaata tcaagtagtt ataggaaatc atgttgaagc tgtctataaa
    10741 gatgtagttg aaataattgg tttagatgat tcaagtacaa gttcagaaac taaaaaatca
    10801 ggtaatatac tagataaagg tatagatata atatcaggaa tttttcagcc agtgttagga
    10861 ataatggcag cttgtggtat gctgaaagga tttaatgcat tatttgtagc aatgggatta
    10921 tatagtgatg tatctggtgg ttatatggta ataaatgcag caggagatgc attatttaca
    10981 ttccttcctt tgttcttagg atatacttca gctaagaagt ttggattaaa accaatgttg
    11041 ggattagcac taggagctgc agtatgttat cctgctatac aaggtagcag tatatcagca
    11101 ggagcagatg tgatgtacac tttatttaaa ggaacaatgt ttgcatcacc agtatatatt
    11161 gatttctttg gactaccaat tgtatcaatt gattatactg gaacagtcgt accaattata
    11221 tttatagtgt attttgcttc taggtgtgaa aaattattta ataagtttgt tccagattta
    11281 gttaagtttt tctttgtgcc aatgcttaca ttattaataa cagtgcctgt tgcacttata
    11341 gttataggtc ctatcgcaac atttggttct acacttattt cacaaattgt aatttcaata
    11401 agagatttta gtccactatt ggcaggtgct atagtagggt ttacttggca aattcttgtt
    11461 atttttggta tgcactgggg atttattcct gtatatataa acaatgttat gactttagga
    11521 tatgataatg ttatgatgcc attctttgct tgtacatttg caagttcggc agttgtgttg
    11581 gcaatatttt tcaagactaa agataaaaaa ctaaaagaga tgacaatacc aaactttata
    11641 tctggtatat ttggagttac agagccagca atttatggaa tattattgcc tttaaagaaa
    11701 ccatttataa taagttgtat tgctagtggg attggaggcg ctttctacgg attctttaat
    11761 cttagaaaat ttataactgg aggtatggga atatttgaac ttccagcaat gattgaacca
    11821 gatgggggaa tgggcaactt aatagtagct ctttctggta tagctatatc aatggtcgta
    11881 gcttttgtat taactatgat tttatataaa gatgagaaag tagaagagtc taaaaaagta
    11941 agaaataagg ggaaagaaga aataaaagaa gtcaagtcta ctaaattaga aagagaaata
    12001 gtagcaagtc ctataaaagg tgaagtatta aaattaagtg atatagagga tgcagcattt
    12061 gcatctggtg tactaggtca aggggtggca ataataccta gtgatggaaa agtagttgct
    12121 ccagtagatg gtgtagttac aaccttattt cctagtctac atgctatagg gattctttct
    12181 gatacaggtg ttgaagtgct tattcatatt ggattaaata ctattcaatt agaagggaga
    12241 ggttttgagg catgtataaa acaaggtgat agaattacaa aaggtcaaac tatattaaac
    12301 tttgatgttg atgcaataaa agagttgggg tatagtactg taactccaat tgttattaca
    12361 aattcatcac aatttttaga tgtagtagaa acagaaagta aaaatattga attagaagat
    12421 aatctaatta cagtcttatt ttagtaaaaa ttgaggcatt agcataagct agtgctttgg
    12481 ttataattta aggaggcttt gactatgagt tttaaggatg attttttatg gggaggagct
    12541 actgcagcca atcaatgtga aggtggatac aatgaagacg aaagaggatt ggcaaatgta
    12601 gatgtatgtc caacaggaaa agatagaaca gcagttatta cagggaaact aaaaatgttt
    12661 gattttgatg atgagcatta ttatccagct aaaactggta ttgatatgta tcataattat
    12721 aaagaagata taaagttatt tgctgaaatg ggatttaaag tatatagaat gagcattgca
    12781 tggagtcgaa tatttccaaa aggtgatgaa gaaacaccaa atgaaaaggg acttcaattt
    12841 tatgaaaata tatttaagga gtgtaaaaaa tataaaatag agcctttagt cactattaca
    12901 cactttgatt gtccaatgca tttagttaaa aagtatggtg catggcgtaa tcgaaaaatg
    12961 ataggtttct atgagaaatt atgcaatgtt atctttagaa gatatagagg attagtgaag
    13021 tactggctaa cctttaatga aataaatatg attttacatg ctccatttat gggtgctggt
    13081 atttgctttg aagaaggtga agatgttgaa aagataaaat atcaagctgc tcatcatgag
    13141 cttgtagcta gtgcaattgc aactaaaata gcacatgaag ttgattcaaa taacttagtt
    13201 ggatgtatgt ttgcagctgg aagtgtatat ccttatagtt gtaatcctaa tgatgtatgg
    13261 gaagctacaa agttagatag agaaaattac ttctttgtgg atgtacaatc taaaggtaaa
    13321 tatcctaact atgcgttaaa gtatatggag caaaaaggta ttacaccaga aatggaacct
    13381 ggtgatattg aattattgaa taaatataca gtagatttta tttcattttc atattacaat
    13441 agccgttgtg ttagaactga tgaaaatgca gatgatatgg ctgaaggaaa catatttaca
    13501 tctgctaaaa atccatatct ttcatacagt caatggggat ggccaattga cccattaggt
    13561 ttgagaatta cactaaatca tgtatatgat agatatgaaa agccactatt tattgtagaa
    13621 aatggattag gtgctaaaga tatagctgat gaaaatggtt atgtagaaga tgattacaga
    13681 attaattatc taagagaaca tataaaagct atgcatgatg cagtttctat agatggggtc
    13741 gatttattag gttatactac atgggcacct attgatttag taagtgctgg tacaggtgaa
    13801 atggaaaagc gttatggatt tatatatgta gatagagata atagtggtaa tggaagtcta
    13861 agaagaatga aaaagaaaag ttttgaatgg tataagaaag taattgcttc aaatggtgaa
    13921 gatttatatt aaacaagaaa tctatgttaa ataagaaatt tgtattaaat aagaagagta
    13981 taaaaattaa taataagtat aatttttaac ttttttaact atagaaaatc aatacagaat
    14041 tattttacat tgtatttatg caatagtatg attcacaaat aaaattttag aaaataaaat
    14101 tgttaagaag tatgggaagt aagtgcaatt tactttgtaa taaaagatga aaaaggcgag
    14161 cttaggtttt aagcttgtct tttttattat gtataaacta tgatacttta aaattctatg
    14221 aaaaatttat ttaatagaga aattaaaaat ttaatggtaa tcaaattctt taccaatgaa
    14281 tataaaaaat gtctacgtgt tataatagtt taaactaata aacgaaaggt agatgaatga
    14341 atatgaaata ccaacatata atagaagttg acaaagaact ttgcattgga tgtgggcttt
    14401 gtaaaaatga ttgtccagta aacaatataa ttatagaaaa taaaaagtca gtaattaaaa
    14461 aacaagattg tctaatgtgt ggacattgtg cagcaatatg tcccacaaaa gctattacct
    14521 tgacaggatt tgatgaacct cctatagaac ttacaaataa gccaaagcta gattcagatg
    14581 aattactaat ggcaattaaa tctagaagaa gtatacgtaa atttaaagac aaagaagttt
    14641 cttcagaaat tgtaaagcaa attattgagg caggtaggta tacccctagt gctaaaaatt
    14701 cacaagatgt atcatatatt gttttagaca ataaaaagtc aatttatgaa aacgaagcag
    14761 tgaaattttt tagaaaaata aaacctattg caaatatagc tattaaatat tcaaaagaag
    14821 ttgagattga cgataacttt ttctttaagc atgctcctat tgcaataatg ataattacaa
    14881 aagataagat tagtggttca cttgcagcat caaatatggc attaatggca gagtcttatg
    14941 gtctaggtgt tttatttagt ggatattttt cagatgtagc aaataattcg ccaaaattaa
    15001 aaaagctttt aggtctaaaa cgcagtaatc atgttcttac aacattagta attggatacc
    15061 cagatgttaa atatagacgt actgcacaga aagaagtagc aactgttaga tacttataag
    15121 agagggtgga ttttatggaa aaagaatgtc aacaaagaat gcaagaaata acatctaaat
    15181 ttaaagaatg tcgcaaagct atctctgcta tgggtgatga gactagacaa ttgattttaa
    15241 ttgctctctt agagagtgat tttaatggta tacgagtggg agaaatcaca aaaaaaacac
    15301 atctttctcg tcctgctgta tctcatcatc ttaagatatt aaaagaagca gagattgtta
    15361 atttgcgtag agaaggaacg aaaaattatt attatcttga ttcaaaaaaa tcacaatgga
    15421 agtcactaac agagctaata aatcttatat ttgttggaat acaacatatt agtgaaaatg
    15481 atataagaaa aggtgattaa attatacttg atttaataca aaaagattga tttaggaact
    15541 aaaaagttca ctttgagaca ctatcttttg aactataccc atcttaggag acagttaaaa
    15601 tatgtaaaac tgtataaaac aatgggtata gttcatttta taatggttta aatattaaga
    15661 atatattatt ctaaatagta tctttctctg ttttgagttt tatttttata ctcaatagct
    15721 tcagtggggc aaccacaaat acatgccata caatgagtac agctattttt ccaaactggc
    15781 tttttgtttt ttaaattaat attatttaaa ggacatagct ctacacattt tccacagcca
    15841 atgcatttat cagtataatg aaatcctttg gcactaataa ttgtcttata aaaaactgta
    15901 tttattatac cacttttaat tgtacctcct aagccactag gagtaatagt ttcaaattca
    15961 ttttcattgc gtatatcaga aataatccta tatatttgtt tatctgcttc tttaatcatt
    16021 tgttttgatg tttctttgtc tgttgtagaa aataaagcta tataattctc tggcatttta
    16081 atttctgcca taccttttaa ttgccattcc ttgtatttac atagtttttg aatatagttt
    16141 atggattttg ctgtatctcc cccacaagtc attacaaagt aaactctgtt gtctcctaga
    16201 aattttgttt cttttataaa atctgtaacc actattggca atctccatgc ataagtagga
    16261 catacgaata tgaatggttt acctgctgat attaattcat ctgttttttc ctcttttata
    16321 agttgattta gtgatatcag ttcatcattt aattcttgtg caattttacg agccacatat
    16381 cggctattac cagtaccgct aaaatataaa atcataataa tatctcccta actatttttt
    16441 attttaactc ttaatttttt atttattata gtactttatt gaaatattct attcaaatgt
    16501 atgtaaaat
//
LOCUS       DAEPOZ010000067        16486 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.067, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000067 DAEPOZ010000000
VERSION     DAEPOZ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16486
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.067"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..1211
                     /locus_tag="KR440_001649"
     CDS             <1..1211
                     /locus_tag="KR440_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0698704.1"
                     /translation="SNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLILGMN
                     KQINSNNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTMHQM
                     KSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLERKY
                     SKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTIDFII
                     AFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIRELE
                     HDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIKQSV
                     LCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDILSKC
                     DIEKVKDILI"
     gene            1352..1660
                     /locus_tag="KR440_001650"
     CDS             1352..1660
                     /locus_tag="KR440_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBH0698705.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            1685..2011
                     /locus_tag="KR440_001651"
     CDS             1685..2011
                     /locus_tag="KR440_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0698706.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            2050..3384
                     /gene="celB"
                     /locus_tag="KR440_001652"
     CDS             2050..3384
                     /gene="celB"
                     /locus_tag="KR440_001652"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBH0698707.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLISSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIVTPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKMLDKQYLREENETVEEEDDDISFDDLVL"
     gene            3395..3913
                     /locus_tag="KR440_001653"
     CDS             3395..3913
                     /locus_tag="KR440_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HBH0698708.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNEKEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCY"
     gene            4009..4968
                     /locus_tag="KR440_001654"
     CDS             4009..4968
                     /locus_tag="KR440_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HBH0698709.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIVDFTFSVV
                     TKEESPAVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            5016..6068
                     /locus_tag="KR440_001655"
     CDS             5016..6068
                     /locus_tag="KR440_001655"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBH0698710.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISKKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKTPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSNTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            6071..6745
                     /locus_tag="KR440_001656"
     CDS             6071..6745
                     /locus_tag="KR440_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBH0698711.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDIGVVKEIGANGVVFGVLDKNNNIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            7298..7849
                     /gene="raiA"
                     /locus_tag="KR440_001657"
     CDS             7298..7849
                     /gene="raiA"
                     /locus_tag="KR440_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBH0698712.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDSEINLADEQEDYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            7981..10656
                     /gene="secA"
                     /locus_tag="KR440_001658"
     CDS             7981..10656
                     /gene="secA"
                     /locus_tag="KR440_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBH0698713.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMVREDLPDSVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            10671..11778
                     /gene="prfB"
                     /locus_tag="KR440_001659"
     CDS             join(10671..10745,10747..11778)
                     /gene="prfB"
                     /locus_tag="KR440_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBH0698714.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            11922..14063
                     /locus_tag="KR440_001660"
     CDS             11922..14063
                     /locus_tag="KR440_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBH0698715.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYNEAVKSMAPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            14211..15377
                     /locus_tag="KR440_001661"
     CDS             14211..15377
                     /locus_tag="KR440_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0698716.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKTLGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     regulatory      15437..15554
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            15679..16284
                     /locus_tag="KR440_001662"
     CDS             15679..16284
                     /locus_tag="KR440_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBH0698717.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
BASE COUNT         6803 a         1722 c         3124 g         4837 t
ORIGIN      
        1 tatcaaatac taagaaagag cttaagctaa aaatgcttat attggatata gaagatgaat
       61 tgaaatctaa tttatccata gtattaaatt atgaagaaaa attgtttata gaatctttaa
      121 tattgggtat gaacaaacaa ataaatagta ataatattaa gaaatcttta tatctaaatg
      181 aagaattagc aagttataca aaaaaaatta taaatttagt atcagaagaa tcaggaataa
      241 attttaataa tgatatatta ctttatgaac aattaatatg tcatttgaat gttacaatgc
      301 atcaaatgaa aagcaatgtt tacttagaaa atccactttt agataacata aaaactaaat
      361 tttgtttttt atttacagta atatcaagtt caatagaaat tgaattaaaa ggaaaacaaa
      421 taacggaaga tgaaattggt tatcttacac ttcactttca aacctctcta gaaagaaaat
      481 acagcaaaag ggaatcaaat aaaaaggcaa gtattgtatg tccatttagt tttggagtaa
      541 gtatgctatt aaaagtaaag atagaaaaga gatttgataa cataaaaatt attgaaacat
      601 taagagagga agatttaaaa agagataact ttaataagac tatagatttt ataattgcat
      661 ttcaagagta tgaaaatatc caaaaaccaa tttttataac aacaccatta tttactgatg
      721 aggatgagaa taaacttaaa gagtttacaa acaaaataaa agaaaaagac acctcatata
      781 aacttatgaa cagattaatg atgtctgatt atttaataag agagttagaa catgatgata
      841 tttatgaagc tataacatat ttaactaatt tattaattga aaaacattat gcagaaaaag
      901 agtatttaca atctattata acaagagaaa agtcatatcc aaccaatgtt ggaaaaggaa
      961 tattattccc tcatggagac atgaaatata taaaacaatc tgttttatgt tttgcaaggt
     1021 taaaaacacc aataaaaatt agaaatggaa aggatataaa gattatactt ttattagcat
     1081 ataaaaaaga tgatgataga aaagtattta gagaattatt taaagaaata tctaatttaa
     1141 cagaggatga aaatatgcta gatattctaa gtaaatgtga cattgaaaag gtaaaagata
     1201 ttcttatata aaattcataa tgaactatgg ataagcacaa aattttccat gtgatggaaa
     1261 aagttgtgca tgtttttttg tttttataag atattatatg tttgtaaggt ataaataatc
     1321 taaaagtaga gaagcaattt aggaggaaaa aatggaagaa atagttttaa agattataat
     1381 tcatgctgga aatgcaaaat ctatgcttta tgaagcactt gactatgcaa aggaaaacga
     1441 ttttaaaaaa gcagatgaat taattgaaaa tgcaaatgaa gaaattttaa aagcacataa
     1501 agtacaaact gaacttatac aaaaggaagc tgggggagat aaatcagata tatctatttt
     1561 attgattcac tctcaagacc atttaatgac ttgtatgagt gagagaaacc ttataaaaga
     1621 aatgattatg ttaagaaaag aaattcaaaa attaaaataa aaaaattaag ggggatttaa
     1681 aagaatgaaa agaaaagtat atttattttg tagctttggt atgtcaacaa gtttacttgc
     1741 agataaaatg cagaaggtag cagatgaaca caatttacca atagaagtag aggcatttcc
     1801 gatagcagaa attgacaaaa tagttgaaga aaaaaatcca gattgtatat tattaggacc
     1861 acaagttaaa tacatgctaa aggagttaaa accaaagttt gaagctcaag gaaaacttat
     1921 agatataatc aatgaagttg attatggaac tatgaatggg gaaaaagtat taaaacttgc
     1981 tataaagcta ataaaatcta aacaagcgta gaattatagt aaaaaataat aggaaatgag
     2041 ggtggataga tgaatagatt ggaaagaata cttatgccat tggctgaaaa aataggtaaa
     2101 aataaattat taattgcaat tagagatggt tttttaatat catcaccatt attaataata
     2161 ggttcattat ttttattagt agcaaatttt cctataaaag gatggaatga atttgtaggt
     2221 caaatttttg gtccagattg ggctgtaaaa ttgcaacaac caactgtagc aacatttgaa
     2281 gtaatgactt tacttgcagt tttaggaatt ggatattctt atgcaaagca aatagatgta
     2341 gatccaatag catcagctgc agttgcaata gttgcctttt ttatagtaac accgtttgta
     2401 ataccatata caccagaagg aacagaatca gtctacatgg taacaggtat accactaggt
     2461 tggatgggct ctaagggaat gtttgtaggt atgataacag caataacctc tgtaaaatta
     2521 tttgctgcag ttgtaaaaaa aggatggaca ttgaaaatgc cagatggagt tcctccaacc
     2581 gtatcaaaat catttgcagc attaatacca agtgcaatag ttatggtagt tttcttttta
     2641 gttaagatag cttttgaagc tacaccatat ggaagtatac atgaatttat atttaagttt
     2701 ttacaaatgc cattattaaa attaggaaat agtttaggtg ctatggcaat cgcttatata
     2761 ttcttacatt tattctggtt ctttggaata aatggaagta gtgttgttgg ggctgtgtat
     2821 aatccaatat taaaaattct atcagctgaa aacttagctg cttttcaggc tggttcaaaa
     2881 ataccaaaca taataacagg tcaattccaa gatatgtttg cgacatttgg tggagcaggt
     2941 agtacactat cattagtcat agctatgtta ttaatatgta aaagtaaacg tataaagtca
     3001 atgggaaaac tatcaatttt accaggaata tttggtataa atgagccatt gatatttggt
     3061 ctaccaataa tgcttaaccc aatactattg ataccatttg caatagttcc aacaataaat
     3121 ataataatag cttacttctg tatgtcagca ggattagtgc ctcttacaaa tggagttcag
     3181 ttaccgtgga caactccaat tatattctct ggttttttaa caacaggatg gcaaggtgca
     3241 gtcttacaat tagtgcttct tatactagga atatttatgt atataccatt tataaaaatg
     3301 ctagataagc aatacttgag agaagaaaat gaaactgttg aagaggaaga tgatgatata
     3361 tcttttgatg atttagtatt ataggaggaa gattatgaag aaaatagtta tgatattgga
     3421 tcaaatacaa gctggagctg gaggaaaaga gaaaagtaat atacctcctg ctggaaaatc
     3481 aagcccatta ggaccaggtg taatgatgga gcaattttta aatgaatcta aggtaatagc
     3541 taccttattt tgtggggatg aattttttgt aaataatgaa aaagaagtaa cgagtaagat
     3601 gatagctatg gtaaaaaaat taaaccctga tgtagtaatc tgtggaccaa gctttaatta
     3661 cgaaaacttt tctaagatgt cagcaatact gtctaaaaat ataaatgata aaacagatat
     3721 accagctttt gcagctatgt cagaagaaaa tatagatgtg ataaacgaat ataaaaatga
     3781 catatgtata gttaagactc ccaaaaaagg tggaattggt cttaatgatt cattaaataa
     3841 catatgtaaa ttggcaaaag caatagcgaa taaagaagat ataactttga tgaaagaaga
     3901 attttgttat taataagagt attaattatt agcaagttta aatattatac tatttataat
     3961 aagcatacat tagtaaaatc tatataattt aaggagggga attttaacat gtggggaatg
     4021 atagcgacat ggagaatggc cctagaagga ataactgagg catcagaaca tttagaaaaa
     4081 ggcatggaag caggtgatgc tgtagagctt gctgtaagaa gagttgaaga ctatccattc
     4141 tataaatcgg ttggatatgg tggacttcca aatgaaaatt gtgaggtaga acttgacgct
     4201 gcatttatgg atggtaacac attagatatt ggcgcagttg ctggtataag agattattct
     4261 aatcctgtca gcatagctag gaagttaagt tatgagaagg taaataattt tttagttgga
     4321 ataggtgctg aagattatgc tcataaaaat ggatttgaaa gaaaaaatat gttgactgat
     4381 agagcgaagt tacattataa aaagagaaaa aaagagactt tagacaaagg attaagccca
     4441 tatgctggtc atgatactgt tggtatgata tctttagaca aaactggaaa aatgtgtgca
     4501 gctacatcaa caagtggatt gtttatgaag aaaagaggta gaataggaga ttctgcacta
     4561 tctggttcag gattctatgt tgatagtagt ataggaggag ctacagctac tggtttggga
     4621 gaagatttaa tgaagggatg tatatcttat gagatagtta ggcttatgaa agaaggaaag
     4681 catccacaag aagcatgtga tattgcagta aatgaacttg ataaacagct tatcaaaaga
     4741 agaggaaaag ctggtgattt atcattagtt gccatgaata ataaaggtga atggggagta
     4801 gctacaaata tagttgattt cactttttca gtagtaacta aagaagaatc tcctgctgta
     4861 tatatttcaa atagagtaga tggaaagact atacataagg ttatatcaaa tgagtggatg
     4921 gaagcatatg aggctagaat aaaagcaccg atagaagtaa gtgagtaaag tagtgttaca
     4981 taattaaaat agagttttat aataaggaga tttttatgaa tatcataagt aataaaatag
     5041 atgagttaaa agaagattta ttaagtgata ttatagatat tgtaaaaata cctagtgtca
     5101 aaggggagtc agaaaatggc ttcccttttg gcgaaaaagt aggggaggca cttaataaag
     5161 cattagaaat atcaaaaaaa ttgggattta aggttagaaa cttggataat tatattggtt
     5221 atgcagagca tggagatagt gatgattatg tttgtgtaat tggacatgta gatgttgtac
     5281 atgaaggaga tggttggaag catcagcctt ataagggaga agaaactaat ggaagaattt
     5341 atggacgagg agtactagac aataaaggac ctataatgtc tgccttatat ggattatatg
     5401 ctattaaaga attaaactta aaattagata agagagttag aataatattt ggtacaaatg
     5461 aagaaagtgg atttgaagat ataccatatt atttagaaaa agaaaaaaca cctattatgg
     5521 gatttacacc agattgtaaa tatcctgttg tatatggtga aaaaggcatg gcaaagatta
     5581 gaataaaaag taaaatcaat tatgaagaag atgtatacct aggttttata gaaaatatgt
     5641 ctgaaaatgt gcttgttaca tataaagagc tgaatataga aaatagtaat actatattag
     5701 atataaaggt aaaatacgat tttagttata aattgaaaga tgtattagat gaaataaaag
     5761 ctagttttcc aaatagtatc gatatagagg taatttctaa ttttaatcct gtatattttg
     5821 ataaagagag taatcttgtt aaaaagcttc agctagctta tgaaagggtt acatcattag
     5881 atggaactcc cgtgacaact aatggaggta cgtatgcaaa agtaatgcca aatatagttc
     5941 cgtttggacc ttcatttcca ggacaaaaag gaatagctca taatcctgat gaatacatgg
     6001 atatagaaga tataatatta aatgcaaaga tatttgcaaa tgcaatatat gaattagcaa
     6061 aggagtagtt atgttagaaa taataggaat gtcagtagaa gacgctaaaa ttatagaaga
     6121 ttgtggtgca gatagaatag agttagttag tgctttaact gaaggtggat tgacaccaag
     6181 ttttggtctt atagaaagtg ttgttaacag tgtaaagata cctgtgaacg taatgataag
     6241 acatcatgcg aaaagttttg tatacagtaa agaagatata agtataatgc aaaaagatat
     6301 aggtgtggtt aaagagattg gtgctaatgg agttgtattt ggtgtattgg ataaaaacaa
     6361 taatatagat gagaaaaatc taaatgtttt attgaaatgt tgtgataatc ttgatgttac
     6421 atttcataga gccatagatg aatcaaatac aatagatagt gttaaaattt tgaaggatta
     6481 tgataaaatt acgaatatat taacatcagg agggaaagga agtatagttg ataacataca
     6541 aatgataaag aatatgatgc ttagttctaa tcacataaaa attcttttag gaggaggtct
     6601 taattttaat aatatagaaa agataaagga attaactaaa gcaagtaatt ttcattttgg
     6661 tacagcgatt agaataaata acagtccttt tgaagatata gatagacaaa agcttaaaca
     6721 actcgtaaat ataatttcaa gatagtatat gaatataaaa tacttagttt aaataaagag
     6781 agtaagttaa ttataatttg ggtatatgaa tgttaatagg tattaaatat gaaattattg
     6841 ttgagtatgc tttataactt ttaagtagtt ttaaatataa ataatgaaag aaaagataga
     6901 ttatggaggt tttagagggt ttatagttat taaaaaatta taaaggtata ttggcatata
     6961 atacacataa tttcctaaaa ttaccatctt atgacatgaa ttcacatatt aataaacaat
     7021 taatggagat ttttgtggat agaaatagga gtaaaaatag ctttaaataa acaaaaacaa
     7081 gagttattaa cagttttatc cacattatcc acaggtgttt gtaataatat taatccacat
     7141 aagtatcaat gtaactaaaa atgataaata attctatcaa ttgaatagaa aaaatcataa
     7201 aagtaaataa taaaaagagg attatgagag ttcgtgtaga atatattatt aaagtataaa
     7261 ttttcttata ctgacataat tgaagggaga tgtatttatg aacataatta tatctggaaa
     7321 acaaatcgaa ttaactgatg caataaaaaa taaaatcgaa actaaactaa gtaagttaga
     7381 taattacatt catccagaca ctgatgtaaa agttacagta agtgcgagaa aagcaagaca
     7441 aaaaatagaa gtaacaatag ctactgtaaa tggtcctata attagagcag aagatatgca
     7501 ggaaaattta tacacagcta tagatgttgt atatgataaa ttaagtaaac aattaaaaaa
     7561 atataaaaag agattacaag ataaacataa agacaacaag agtataagat tccaagattc
     7621 agagataaat ctagctgatg aacaagagga ttatggggat gaaaatcaag aattagtaat
     7681 tcaaagacgt aagaaattta gtgttaagcc aatgagtgaa gaagaagctg tattacagat
     7741 ggaattaata ggacatgatt tttatatgtt caagaacata gattcagatg aaatagcagt
     7801 agtttataag cgtaataatg gtggatatgg tataatagaa catgaataaa gcaatttaga
     7861 tttaatcata ttagctatcg ataaataaat tatcgatagc tttttaaatt aatattaaaa
     7921 gtatagtttt tatggtaaaa tgaataatag tacaataaag aacgaggaga gatgtaaaat
     7981 atgagcttta tggataattt attcaacatg gcagataaaa aagaattaaa aaagttcaat
     8041 aaaactgttg atataataga ttcattagaa cctaaatttg aatctatggc agactcggaa
     8101 cttaaaaata tgacaaatat atttaaagaa agattagcaa atggtgaaag tatagatgat
     8161 atacttccag aagcttttgc agtagttaga gaagtttcta agagagtgtt aggacttaga
     8221 cactacagag ttcaaatgat tggaggtata gtactacatc aaggaagaat agcagaaatg
     8281 aaaacaggtg aaggtaaaac tttggttgca actgctcctg tttatttgaa tgcgcttact
     8341 ggaaaaggtg tacatgttgt aacagtaaat gattaccttg caaaacgtga tagagaccaa
     8401 atggcaaaaa tctatgagtt tttaggaatg agtgtaggcg ttattataca cggtcaaaat
     8461 ccaaaagtta gaaaagaaca atatgattgt gatataactt atggtacaaa taatgaatat
     8521 ggatttgact atctaaagga caatatggta atacacaaag agcaaagagt tcaaagagga
     8581 ttaaattatg ctatagtgga tgaggttgac tctatactaa tagatgaggc tagaacacca
     8641 cttatcatat ctggacctgg agacaaatca actcacttat attcagatgc aaacacattt
     8701 gttttaacct taaaaccaga tgattatgaa ttagaagaaa aagataaggc tgtatctcta
     8761 acagcatcgg gtatacaaaa agcagaagtg tattttaatg ttgataatat aacagatata
     8821 tctcatacag aattatacca tcatataaat caagctttaa gagcacatgt aataatgaag
     8881 aaagatgttg attatgttgc taaagatgga gaaatagtaa tagtagatga gtttacaggt
     8941 agacttatgt ttggtagaag atattcagaa ggtttacatc aagcaataga ggctaaagaa
     9001 ggtcttaaaa tacaaagaga atctaaaaca ctagctactg taacattcca aaactacttt
     9061 agaatgtata aaaaactttc tggtatgact ggtacagcaa aaacagaaga agaagagttt
     9121 aaagcaattt ataaaatgga cgttttccaa gtacctacca ataagcttat ggtaagagaa
     9181 gatttaccag attctgtata caaaagtgaa ataggtaaat ttaatgctgt agcacaagaa
     9241 ataattgaaa gacataaagt taatcagcca atacttgtag gtacagtatc gatagaaaaa
     9301 tcagaattat tatcacaaat attaaagaaa aaaggcatta aacatgaagt attaaatgct
     9361 aagcatcatg ataaagaggc agagataata gcacaggctg gtagattagg cgctgttaca
     9421 atagctacaa atatggctgg ccgtggtaca gatatagttt taggtggaaa cccagatttc
     9481 ttaactaaga gagaaatgag aagaaatgga tttaaagagg aaatagtaaa tagggtagat
     9541 actccaatag aaggtatacc tgtaaaaggt aatgaaattc tatttgaagc tagagaagag
     9601 tatgaaaaac tatttgaaaa gtttaagcaa caaacacaag aagaacaaaa acaagttgta
     9661 gaagcaggtg gacttgctat aataggtaca gaaagacacg aatctcgaag aatagataac
     9721 cagttgagag gtcgtgctgg tcgtcaaggt gacccaggga gttcaagatt ctatataggt
     9781 cttgatgatg accttatgag attgtttggt agtgatagaa tatctggtat tgtagataaa
     9841 ataggtcttg aagaagacat gccgatagaa catagaatat tatctaagtc tattgaagga
     9901 gcacaaaaga aagtcgaagg taaaaacttt ggaataagaa agcatgtact tcaatatgat
     9961 gatgttatga ataaacaaag agaaataata tatgcagaga gaaaacgtgt attagaaggt
    10021 gaagatttac aagaacaaat acaatcaatg actcattcta taatagaaga agcagttact
    10081 ctttatacac aagataaagg atttgatgaa gaaggattca aagaacatat gtataatctg
    10141 ttcttgccaa aaggtagcat agaaatacca gaaatagaaa aacttaatcc agttgaaata
    10201 acagaaaaag tttatgaaat agctatgaag atatatactt caaaagaaga acaagttggc
    10261 tatgagagaa tgagagaagt tgagagagta atcttacttc aagctgttga taaccattgg
    10321 atagaccata tagatgctat ggaccaatta cgccaaggta ttggtcttcg tgctgtaggt
    10381 cagcaagacc cagttattgc ttataaaatg gaaggttttg atatgtttga tgaaatgaat
    10441 aaacatatca aagaagacac tgtaagatat ttatttaata taacaataga aactccagtg
    10501 gaaagaaaag cagttgtaga tgttgaaaat ctatcttctc catcagatgg aacacttcca
    10561 acaagtaaaa ctgttaaaaa agatgaaaag gttggaagaa atgacttatg cccttgtgga
    10621 agtggtaaaa aatataaaaa ctgttgtgga agataatctc ggaggattaa atgttaaagt
    10681 tacaagagta tagacatagt atggagcaga tggcttcaaa tttagaagaa ttgagggttt
    10741 ctctttgaca tagcttcatt gatgaaaaat atacaaatta atgaagaaaa gatgaatcaa
    10801 caagactttt ggaatgataa tgaagtcgct caaagagtac ttcaagaaaa taaatcttta
    10861 aaagagacat tagaagaata tgaaagctta aaaagcttat tagaagatat agaagtatta
    10921 attgaaatag gtttagaaga agatgatgat tctgtagaaa gagatataga aaagtccata
    10981 gaatcaatgg aagaaaaatt atctgaaatg aaaataaaga ctcttctaaa tggagagtat
    11041 gataaaaata atgctatatt atctataaat gctggtactg gtggcttaga tgctcaagat
    11101 tgggctcaaa tgcttttgag aatgtatata agatggtcag aaagtaaggg ttataaagtt
    11161 aagcttttag atataatatc agaccctgaa gcagggataa agacagctac aattcttgta
    11221 gaaggaacaa atgcttatgg gtatttaaag agtgaaaaag gagttcatag attagtgaga
    11281 atatctccat ttgacccatc agggaaaaga catacttcat ttgcatctat agatgtaaca
    11341 ccagagttag atgaaaatat agaagtagaa attaatccgt cagatttaaa gatagacaca
    11401 tatagagctt ctggagctgg agggcaacat gtaaatacta ctgattcagc agttagaata
    11461 acacatatcc ctactggtgt agtagttcag tgccaaaatg aaaggtctca gcatctaaat
    11521 aaggacagag ctatgagact tttaatggcg aaattgatag aattaaaaga gttagagcaa
    11581 aaggaaaaaa tagaagatat acaaggcaaa tactcacaaa taacttgggg tagtcaaatt
    11641 aggtcatatg tttttcaacc atataaattg gttaaggacc atagaacaaa tgctgagttt
    11701 ggaaatgtag atagtgtaat gaatggaaat atagacttgt ttattaatga atacttaaaa
    11761 atgaataaaa tagtttaata aatatgcact gtaattaaga tatatgtata agaaggttag
    11821 tgcttaatat gcacttacag cacttaggct gaggacgata accctcagcc ttcgttctgt
    11881 tttgttatac ttatgaaaaa ctaacattag gagtgaataa attggatata aatcaaatct
    11941 taaaaaaaga gtttaatctt agagatgaac aaataaataa tacattaaaa cttattgatg
    12001 aaggaaatac aataccattt atagctagat atagaaaaga aatgactggc gaaatgagtg
    12061 atgttacact aagagaattt tatgaaaaat taatgtattt gagaaatctt caaagtagaa
    12121 aagatgatgt agttagacta atagatgaac aagggaaatt aacagatgaa ataacacaaa
    12181 atatagaaaa agcaaaaaca ttacaagaag tagaagatat atatgctcca tataaacaaa
    12241 agaaaagaac tagagcaaca atagcaaaag aaaaaggatt agaaaattta gcattgtcaa
    12301 tactagagaa taatttagat aatatagaaa tagaggcaaa gaattatctt gatgaagaga
    12361 aagaagttct ttctattgaa gatgctctaa aaggtgctag agatataatt gcagaactag
    12421 tctcagatga tgctaagatt agaaagtata taagagaatt agctttaaga gaaggtatga
    12481 tagttagcaa aagtgctact gatgagaaat cggtttatga tatgtattat gattataacg
    12541 aagcagtaaa aagtatggct ccacatagag ttttggcaat aaacagagga gaaaaagaaa
    12601 gcttcctaaa ggttaagtta gaaataaata atgataaagt tttaaattac ataataaacg
    12661 aatatgtaaa tgataaaaat ttcaaaaata aagaagaaat cgtaagttca atagaagatt
    12721 cttataaaag attaattttc ccatcaatag aaagagaaat aagaaatcat ttaacagaaa
    12781 tagctcaaga aagagctata agtgtatttg gaaagaatgt taaaagttta ttgcttcaac
    12841 caccagttaa agacaaagta gttatgggat ttgaccctgc atttagaact ggatgtaaaa
    12901 tagctgtagt agataaaaat ggtaaactac tagattatac aacagtatat ccaacagacc
    12961 ctcaaaatga tgtagaggga gcaaagaaag ttttaaaagg attgattgaa aaatacgata
    13021 tagatataat ttcaattgga aatggtactg catctagaga atcagaaact tttgtatcag
    13081 aaatgataaa agaaatagat agcgaagtgc agtatgtgat agttagtgaa gcaggagcat
    13141 ctgtttattc agcatctgaa cttgcaaatg aggaacatcc agatataaat gtatcaataa
    13201 gaggagctat atctatagct agaagattac aagacccatt agcagagctt gtaaaaatag
    13261 accctaaaag tattggtgtt ggtcaatatc agcatgattt aaacaaaaag agattagaag
    13321 aagtgctaga tggagttgtt gaagattctg taaatagtgt tggagtagat ttaaataccg
    13381 catcatactc cctactagaa catgttgcag gaataagtaa agctatagca aaaaatataa
    13441 tagcatatag agaagaaaat ggagatttta catcaagagc acaattgaaa aaagtaaaaa
    13501 gactaggacc tcaagcattt actcaatgtg ctggatttat gagaatctta gagggtaaaa
    13561 atccactaga taatacaggt gtgcatcctg aaagttatga tatatgtaaa aaaatgattg
    13621 agataattgg atattcttta gatgatgtaa agaataagaa tataggtgaa atagatgaaa
    13681 aaataaaaga aattggcttg agggaattaa gtgaaaaact ggaagtagga caagttacgt
    13741 taaaagatat catagctgaa atcaaaaaac caggaagaga cccaagagaa gaaggtataa
    13801 aacctatact tagaactgat gtcttaaaaa ttgaagatat acaagagggt atgactttaa
    13861 aaggtacaat aagaaatgtt gtagactttg gtgcatttgt agatattgga ataaaaaatg
    13921 acggattagt tcataaatca gaaatgagta atagttttgt gaaagaccct atgagtattg
    13981 ttacagtagg tgatatagta gatgtaaaag tcattggaat tgacttaaac aaaaaaagag
    14041 ttgccttatc aatgaaaaaa tagataagtt tatgtatggt tatagtactt aaattctata
    14101 tttgtaaatg agattactcg agtgtataaa tgttaaaaat ttgtaaaagt atatagtaag
    14161 aaaatatatt aaaataagaa cgatagtctt gaaaatcagg aggaaaatga atgattttta
    14221 taaaaaatgg taaaataaat accataacaa atgggataat tcatggagat atacttatag
    14281 atgaaggaaa aataatagaa attggagaag atttgatagc tcctttagat gtagaggtaa
    14341 tagatgctag taataaactt gtgtttcctg gatttataga tgctcatact cacttaggtt
    14401 tatgggaaga tggtataggg tttgaaggag cagatggaaa tgaagagact gacccaataa
    14461 caccacaatt aaatcctata gatggaatta atccaatgga tagaactttt aaagaagctt
    14521 ttgaaggtgg aataacttct gtttgtacaa cccctggaag tgcaaatgtt atgggtggtc
    14581 aatgtatagc tataaagaca tgtggaaaaa gaattgataa aatggtaata aaaaatccag
    14641 ttgcatctaa aattgctttt ggagaaaatc caaagagctg ttatggtcag gatgataaat
    14701 ctcctcaaac tagaatggct atagctgctt tacttagaga aaacctaaaa aaagcagaag
    14761 aatatttaga ggatattgat atgtatgaga gtcatgatga tgaagactgt gaaaaacctg
    14821 aatatgatat aaaaatggaa agtttaatac cagttttaag aagagaaatt ccttttaagg
    14881 cacatgctca tagagcagat gatatgttta cagcaataag aattgcaaaa gaatttaatt
    14941 taaagcttac attagaccat tgtacagaag gtcatcttat tgtagatgaa ttagtggaag
    15001 aagggtttcc tgtaatagta gggccatctt tgtcagaaag gtctaaattt gagcttagaa
    15061 atttgacatt taatacagca ggaatacttt caaatgcagg tcttgatgta tgtataatga
    15121 cagaccatcc agtaatacca gttcaatatc ttccaatatg tgcaggtatt gcagtaaaac
    15181 atggtatgaa agaagaaaaa gcaatagaat caattacgat aaatccagct aagactttag
    15241 gaatagaaga tagagtaggc tctatagaag ttggtaaaga tgcagaccta gtaatttggg
    15301 ataattctcc actagagatt caaagtaatg tactttacac tataataaat ggcaaagtag
    15361 tgtatgagaa aaaataactt gacaattatt aaaaaattaa ctaatataag agtgtaagga
    15421 aaattaaaaa atcaaagaag atcttcgggg cagggtgaaa ttccctaccg gcggtatagc
    15481 ccgcgaacta cttaggtggt tgatttggtg agattccaaa gccgacagta aagtctggat
    15541 gagagaagat atttatggaa gataaataag tttattttag tttgtttaaa ctatatccat
    15601 aaaaactcga agatagggtc ttcgagtttt ttgtttttcc taaaaaatat aaacatattg
    15661 attaggggga aaataattat gcaaaacaca gcaaaaagag gcaatatgat ttcaacaaga
    15721 actttagtag taatgtcaat tttatctgca atatcttatt tattaatgtt tattcaagca
    15781 ccaataccag gtatatttcc agatttctta aaaattgact tatcagatat tccagcaata
    15841 ttcggaggaa tgtctctagg accattagtt ggatttggaa tagttgtagt aaaaaatgtg
    15901 ttacaagcaa taacagcaac aacaactgga ggaataggag aatttgcaaa tatattaatt
    15961 ggtggttctt atgtaatgat actttgttat tcatataaga gaagtggaga tttaaaaggt
    16021 gtactgattg gttttgtatt aggaacaata tctatgacta taattggttc attagtaaat
    16081 tattttgtaa tgttgccact ttatggtcaa cttatgggac tagatgctat aataggttta
    16141 ggttcagcta taaatcctca agttcatgat ttattcactt ttgtaatttg gatgatagct
    16201 ccatttaatg tgctgaaggc tgttataata tctttagtaa cattgccact ttataaaaaa
    16261 atgggaaata taataaagaa ataattagac aagaaaatat aaaattaaat gtattgaata
    16321 gccataattt atttataact tcagtcctat attgtgattg atatattaaa tatattatgg
    16381 ctttattaat ttatcaaaaa atgtatataa aataagaaat atttagacaa atttaaattt
    16441 taaaataaag gaagaattaa aacgattaac tattaaaata tgaaaa
//
LOCUS       DAEPOZ010000068        16439 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.068, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000068 DAEPOZ010000000
VERSION     DAEPOZ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16439
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.068"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(87..1373)
                     /locus_tag="KR440_001663"
     CDS             complement(87..1373)
                     /locus_tag="KR440_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBH0698718.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAISENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(1496..4594)
                     /locus_tag="KR440_001664"
     CDS             complement(1496..4594)
                     /locus_tag="KR440_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBH0698719.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGINDISHVK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRKKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKFKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFIVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMEVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKHLIHPDDIILFDDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDILTGLYN
                     YESFCEQTSLMLKRYPDKSYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDVHKLIYKIKNSLNRKKFEYMLVPCFGVYKILDNN
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLRHTIAMAKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
     gene            5318..5470
                     /locus_tag="KR440_001665"
     CDS             5318..5470
                     /locus_tag="KR440_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698720.1"
                     /translation="MKIFSFLLFVVGICFCIAGFMGHYPAFAGATLSFFATTMIAVRQ
                     VKTKKK"
     gene            complement(5762..6466)
                     /locus_tag="KR440_001666"
     CDS             complement(5762..6466)
                     /locus_tag="KR440_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0698721.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(6457..8064)
                     /locus_tag="KR440_001667"
     CDS             complement(6457..8064)
                     /locus_tag="KR440_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HBH0698722.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(8318..9496)
                     /locus_tag="KR440_001668"
     CDS             complement(8318..9496)
                     /locus_tag="KR440_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698723.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVGAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(9722..10216)
                     /locus_tag="KR440_001669"
     CDS             complement(9722..10216)
                     /locus_tag="KR440_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBH0698724.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(10217..10978)
                     /locus_tag="KR440_001670"
     CDS             complement(10217..10978)
                     /locus_tag="KR440_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBH0698725.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
     gene            11701..14535
                     /locus_tag="KR440_001671"
     CDS             11701..14535
                     /locus_tag="KR440_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0698726.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHVSIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNISKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNIRKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            14578..15822
                     /locus_tag="KR440_001672"
     CDS             14578..15822
                     /locus_tag="KR440_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBH0698727.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKV"
     gene            complement(16263..>16439)
                     /locus_tag="KR440_001673"
     CDS             complement(16263..>16439)
                     /locus_tag="KR440_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HBH0698728.1"
                     /translation="TYKTAIDEAQAILDKADATQKEIDDAVTALNTATATFEAGKVPT
                     TIALMLSRILGFMK"
BASE COUNT         5730 a         2727 c         1880 g         6102 t
ORIGIN      
        1 tttcatgata agctttaata ctttatatag taatttgttg tagaatttta taaaatataa
       61 tgatttatac agttttataa aatatcttaa aacagtattt cagtaatatt ttttggtctt
      121 cctccaccaa tactagtctc ttttgcacat attcgaccta aacctgcatt tactatttta
      181 tttaatatac gtcttgcact tctttcactt acatttagat actctgctaa ctcttttgaa
      241 tcataaatct tactttttct attctcactt atagcaataa tttttgcaac tgactcacaa
      301 ctcattccag tctttttaga tatttctatt accttttcat cagatagcat caaagaataa
      361 tttaattcat tttcggaagc taaaggtcct cttatatttt catcttcatc taccaaatat
      421 ataccaattt cttttgaatc tatggagtgt cttaatgctt tatgtgcatt attttcagcc
      481 tgatacgcag ttactccagt accaattcct atactaagag aaaagcccat gtcttttatc
      541 tctctcttta gattgaatag cttatcataa ttatactcat tatcagctac ccctttatga
      601 acaaatacaa cataatcatt tcttccaagt aaaaataagg aaccttgaat gcttcttaca
      661 tagtttacta caagcttatc catatctgat ttttttatca tgtcagaata atagttatct
      721 atcttctctt tataatctat tagactcaat atttcgacag caatttgaga aaatcttgcc
      781 ttgcttagag catattcact ctttacctta tcataacttt ctttgattaa ttgtatagta
      841 gcttgtaaac gaaatacagg atagcctctt tcttttaatt cattatatac tgcaccaaaa
      901 ccagttaaca ttatatccgt cttattttct tcaaataatt ttatatgcca ttctacaagt
      961 tcagactctc ctacttccaa agaaaatgga ttacaaaata cactcgttgc tttaatatca
     1021 aattctttaa gtgcatcttc cacaacatat ctatcaacta catctatact gaatttatct
     1081 aattttatat ttgcactttt tattgcccaa aatgccttca ttatactcgt cccacttctc
     1141 ggtacaaatg aatgtggctt acttatctca tacttagatt ttataaattc ataaactgca
     1201 catcctgtaa atataatagc atcacattca tcttcacatt tagaaataac ttctattgta
     1261 tctactactt tttctcttac atatatttta gtctttaaat cactgtctat ctgttttaaa
     1321 tcatccttta tcttaaaccc agagtctgaa ggacctataa tacccactat cataaacttc
     1381 gctcctaatc cataaatatt atacttttta aaaattatat cctcactatt ttctacttct
     1441 atttaattat gttgaactac tccatcctac ctacatttag agtgggataa ttagcttatt
     1501 tttgataaag taacttctca aaatcagcac ttggaatagg cttacaataa taataacctt
     1561 gaattaccac acaatctaaa ctcactaaaa attcagcttg ttcttttgtc tctacacctt
     1621 ctgccactac atccatatct aaatcttttg ccatggctat agtgtgcctt aaaactattt
     1681 tttctctgtc tgtgattcct tcctcattaa agaagccaca atctatcttc aatgtatcaa
     1741 tattaatatt ttttaacata tttaatgaag aatatgctga accaaagtca tccatattta
     1801 aaacaatacc atccattcta aggtcggcta ttatcttagt aaataatttt atatttttaa
     1861 taaataaact ttctgtaagc tccaattcta aaaaagattt tggtatacta tatttattta
     1921 ttaaacttaa aacaatttct ttaaaatgtg gattatgcaa atgtaatctt gatacattta
     1981 cagagatagg aactggctct tcacctaaat ctatccattt tctaatcaaa gaacatactt
     2041 gttcccacac atacatgtct aatctgataa taaacccatt cttctcaaat agtgaaataa
     2101 atttaattgg aggtattata cctttttctg gatgaaccca tctaaccaat gcttccgcac
     2161 caacaatttt atttgcatta agattatact taggttgtag atatacatgg aattgcccat
     2221 tttcaagtgc gtattccatt tccctttcca tctgtttttc ttcaagtgca tcatttctta
     2281 tagtatcatc ataaaatata tagttattga tactttgttt ctttactttt ttgtgagcat
     2341 atcctgccat ttcacacgca tttgtaattg gaatattatt atccaaaatt ttataaaccc
     2401 caaaacatgg tactaacata tattcaaatt tttttctatt taagctattt ttaatcttat
     2461 aaattaattt atgaacatct ctatcagagt aactctcata aaaaatacaa aatacatctg
     2521 catacatacg acaacatata ccattagaac caatgttttt ttgaatcaat tctgcaacat
     2581 atattaaagc cttatctcct cctctaactc cataaatgtc attaattacc ttgaacttat
     2641 ctatatccat cattataatt gcatagctct tgtcaggata tcttttaagc attaaacttg
     2701 tttgttcaca aaaactttca taattgtata gtcctgttaa tatatccttt tggtttttat
     2761 gtagttgcat aagataatct atatttttta tagcaactaa aatatgtgtt tgtttctcac
     2821 ctaacttatt tttactacta atctttaaca tataaattaa tgcccaatag tactttttat
     2881 ctattcctaa acgtctatac tcaaaattta gttctaaatc gtcttgcttg ctgttattaa
     2941 gactatcaag agattttaga aggtggttat catcaaataa tattatatca tcaggatgaa
     3001 ttaaatgttt ttttatgtat gaaaaagttt tttcaagatt ttcctttata ggaaaactaa
     3061 aaatattgct ctctgattga taaattggag taattaaatt agtatcgatg caaatagata
     3121 aaattatatc atatgtatta taagctaatt tttcataagc cttagaatac ccttttatca
     3181 ttaaatcatc aaaataaacc ttatcaacta actctttatg tttaaataaa atattattcg
     3241 tcgaatcagc taagtcacta attacttcca tttcattatt tctaagtttg ttcttattat
     3301 aatacctctt cttatcttgg tacattctct catcagcaac tttaattagc ttatgtatat
     3361 caatattatc gtcactccaa atactgccca ctgctacact atatatcatc tcatccttaa
     3421 attcttcact taaccaactc actaattctt caaatagttg ttttgatata tcttcacata
     3481 atacaataaa ctcatctcct ccaatacggt aaacattata agaatgaaag tattttttta
     3541 ttattacaca tgctgacaca atagcagagt cgccattagc atgtccaaag ttatcattaa
     3601 tatcttttaa cccatttata tctacaaata caactcctat ggatttaaat tttccttttt
     3661 gaaaattatc tatatactta atatatttgt ttctattata tgcaccagtc aagaagtcat
     3721 agtagctcat ataatttaat ttattttcta cttttctttt tttcatctca gttaataaaa
     3781 aataagatag ggatgtcaaa agagaggtat ctccaacagc cttatgtgga ttatcaactc
     3841 caataaaccc tactacttta tcatcttgtt ttagtgaaga tgtcattaaa ctttcaattt
     3901 tttgaggttt taaaatttca tattctattg gctgttcttc ttttatattt tctatatcat
     3961 caattattac aaaacccttt tgttcgaata ataaaaacca attctttaat tcattaatat
     4021 caagatttgt tagtgcctgt ttttgttcat caacaccttc attgcaccat tcatatgtat
     4081 tagttacttt ttttctatct tctgatactt caaaaacata tgctctatca gctttatgaa
     4141 atttaccaat attggttaat acaagattta cagcatcaga aaaattagat tctgttataa
     4201 gatgtttaat acatccaact aataactcct ttatctctaa tttttctgct attgacttac
     4261 ttatatcttc cttttctgta atatcaagag ctatttctaa tcgtgcatgt ttgccctccc
     4321 atttaattaa cttgtccttt aatataaaat gtctatttgt tcgagtattc gtattttccc
     4381 atatgtaata ttcatcttct tttaaaatag agtttgtaca aaattcgcag ggagaatctt
     4441 tcccctgtaa tgctttataa cattttacat gggatatatc attaattccc attgcctttt
     4501 tcccaatttc attaatatat aaaagttcat atgtatctaa agaagaaaca tatacaattt
     4561 catccaactc atctattaaa tttattacat ccatttatat atatctcctt ttattcattt
     4621 tccagattga atttttaaag cttttaaaat tttgctatat atttggcaat gatattatac
     4681 ttctttatgt tttaaaatag taccttaata aagttctatt ttttagtaaa tagaattatt
     4741 attgtgtcaa tcgtacaata gagttttgta atacctaatt taatatattt ataattaact
     4801 atcttattat gtgtttacaa ttaattctca tataaatgac aaatctccac ctcaattaag
     4861 tattccaatc atatatgaag ataatacttc attcatttaa aaatataaaa ctattatata
     4921 ttaaaatttc ttcaataata aattatctag attgttactt tgatatatta tataatatca
     4981 caaaggctga tataaaaaga caaaaagggt aattttttta tatcaaaaaa aatgttaaat
     5041 tgatattatc catatataaa ttgatattat ccatatagat gctatattta acaattctac
     5101 ttacttccct tacacttatt agcatatagt tattaagagt ttgttgcaaa acaacactta
     5161 gcacacttta aaaagtgtgg tctaataagt aatcaacgaa gaagacataa taaagcataa
     5221 aatatgatat acttttcctg tcatcaaaac tgatgatttt actattaata tctgctaaaa
     5281 tatgcttaat aaattaaatt ttagtgggag aatagttatg aaaatattta gtttcttgtt
     5341 gtttgtagtg ggaatatgtt tttgtattgc tggatttatg ggacattacc cagcatttgc
     5401 tggtgcaaca ttgagttttt ttgcaactac aatgatagct gtaagacaag tcaaaactaa
     5461 gaaaaaatga aataggcaaa tcctaacttt acaagttaat attacataag taattagtct
     5521 aaaattggat actttttcta ttacagaaat acatcatcca ctttgtattc tcttaaaaaa
     5581 ataaaatagc tatgtaaatt caagattaga tagattaact ctctatccat ttcattgtaa
     5641 aatatacata gctatttgta ttcgatttaa aatacttaga ttttcttaca taacaaataa
     5701 tttctatctg attattactc atattcattt agtaatcact cagcaactaa tcatttagta
     5761 tctattcatc cgccatacgg taacctaccc caacctcagt caaaatatat tctggctgag
     5821 caggattctt ttcaattttc cttcttatac ttgccataaa aactctaagc gactgatttt
     5881 cactttcata gtaatttccc caaactttat ctattataaa cttatgagtt aagactcttc
     5941 ctgaatactt agacatcaac tcaataatct tatattcgat tggagttaaa tgtatttctt
     6001 caccattaat tgacacaata tgattttcat aatctatctt taggttttta acttcaaaag
     6061 actttggagc atcattattt atattatcct gttgagcttt atttctaaga gccacacgta
     6121 ctctagccaa aagctctgtt acactaaaag gtttagttaa ataatcgtct gctcctccat
     6181 ctaaagcttc caccttatcc ctctcatgct ctctcgctga aacaacaatt attttattag
     6241 tctttgcaca tgccctaact cgtcctatta cctcaatccc atcaatatct ggtagtccta
     6301 aatccaaaat tatcaaatct gggtcaagag aatgtataag tgatatagct gttcctccat
     6361 tatctgtctc tacacaccta taattttgag tttcaagaga tacttttata aatttacaaa
     6421 ttggcttatc atcttctaca attagaacta atggctttat ttccacttgt ctttcccctc
     6481 ctcatcatac ataggtatgc taaatctaaa tgtagctcct ccagaaggat tattaaatgc
     6541 gacaatttct ccaccatgag cttctactat agatttacat atctccaatc caagaccaat
     6601 acctcttctt tcaagatttc tagacttatt cttagtataa aacctattaa aaatattacc
     6661 gatatcttca tttggtattc cttttccatt atctgcaact tcaaatagca cttgatttga
     6721 ctcttttttt acagttagtt ctatcttaga atttttaggt gtatgcctta tagcattatc
     6781 aattaaattt actaacactt gttctattaa taatacatcc acatgaacga ttattatctc
     6841 atctggaata ttggttttta tgtctctact attggcaaac tttttgactc ttaatataga
     6901 ttcagctatt atttcatcaa caacttctgg tcgtttttca atatccaatt tcccttcatc
     6961 tattctagtc atactaagta tattctcaac agaatgagtt agccaactcg tatcttcata
     7021 tatatttttc aataattcta ctctagtttc tttatctatc acatcatcat tttcaagtat
     7081 tgttgaagac gaacccaata tacttgtaag tggagttctt aaatcatgag aaattgacct
     7141 aagcaaattt cctcttaatc tctctctctc tgcctcaaga tttgcttttt tatttttctc
     7201 aaataaattt tctctatcaa ttgccaacgc aacctgtgtt gaaactgctt ctaatagtat
     7261 cttttcattt tctgtaataa tatctatctt aaaacacgca acacctataa caccaagaac
     7321 atttgattct tgacctttta tagggaaata atatgcactg ctgtttttgc aatattttgt
     7381 cccaattcca actgggttac ctgtcctaaa agcttctttt atagcttctt tttctaatac
     7441 tgaatggaat atattctcac ttccatcatt gttacaaata taaatacttg gcttacctag
     7501 attattattc tcatctgcaa tagtcactat tacagttcta tttaatatat ttactaaatt
     7561 agacccacaa aattcaataa tttgattttt attttttgct ttaagcaacg ttttgttatt
     7621 ctcatagagc aatcttatca ttttttctct cttatgagaa agtctcactt ctctttttat
     7681 cctagaagtt aaagtacttg ttataattgc tgccattagc ataatacaaa aagtcatagg
     7741 ataatctttt ctataagcta aaaaagtata taatggatgc gtaaagaaaa aattaaatga
     7801 tagcactcct attactgaag aaagtattcc aaaaaaatat ccataggtta tacttgtagc
     7861 aaatagtaca cctaatatgt atactagtat aatattagat tcattaaaac ctaccttttc
     7921 aaaaactact gatattccag tagcaattgt cataaccaat atagttttta tgaagtcctt
     7981 aataattatc tttttactga gatagccatc ttcatcataa tcatactctt ttaagtcttc
     8041 atgttgtagt cgttcctttt tcatttacta caattcctcc ttattgttac tgtttaaatt
     8101 ggctctattt tatataatac aattttatca taataaattc aatcaataca tttaattaat
     8161 gtacttaaat tactaaaagg tgctgtctta aaatgaaaat aaatctctat ttcaaaacag
     8221 cacctatata tttaaattac taatttagtt tttacatact taaatcaata gttttattca
     8281 attttataaa ttataatact tcaattatgg aacatttcta tccaacaagc ataaatataa
     8341 agtgggcaca tatacctgca cacaaaccac ctatggtatg cgaaagtatc gctttaccag
     8401 ccaatggtct tgcatctaaa gcatccatca tacctatgtg tgtacttaaa tatccactcc
     8461 aacacatacc cattgctgtg aatactgcta tatcattagg agttatagcg cctgatttta
     8521 taaactctgg tactaaagat attgctgctc caacagctcc taatgcagtt acagggaatg
     8581 ctattgcctc tggtgatgtg aatccaaata atggttctat tatgaagcta atcttatcac
     8641 caatagcagg taataatttt ataccttcat atgcagctcc tgtgtaaaca gcttgacctg
     8701 tagcagggtc tgtagatggt ccaaaagtta ataacataac taatgtacaa actactaaaa
     8761 ctcctggtat tatagccata cccatttcaa cacctagctt accaccttca agtatagcat
     8821 ccaatgttct ttggaataca ttaccatctc ttatttctct aaactcttca agttttgcac
     8881 ctttatcatt tatttgcttt tcagtatcat ttttagggtc ataattataa tactttttag
     8941 taaaagtcaa cataattcta acactaataa tactacctat tattgcccca acattaccta
     9001 ttatagctgg tagtacatat tctttacctt gagctatcat aaaagttgtt acaataagtc
     9061 ccataccaaa ggctgttcct aaattacata aagcaggtac ttgatacttc ttaaaatatt
     9121 gtgtaaatgt tttgtcttta gcaaatggta taatagctgg attatcagat aaatatgttg
     9181 caactactcc tgctatacta gcaccaggta agccccatat aggtctcata agacccttaa
     9241 atatcttatt aactaaagat attactccaa actctgaaag caacgcacta agtgcacctg
     9301 caagaactgc catagccatt attaaaaata ctgtgtctaa taaaagcgca tgtgctgtac
     9361 tcattattac tttaaacatc attcctgcac ccatgatgct tcctacatat ccaaatccta
     9421 ccgccaatag tacaataaat acgaaggttt ccatacttac tgccttgact cttctcttat
     9481 ccgacagctt ttccatcttg aaatcttccc ccttttaatt ttaaattaag gttcctaaca
     9541 ttggtacaaa ttttttggta caaactgagt tctaaaactt acactcgtaa tgcaagttgt
     9601 cagttttttg aaataaaaaa aaattacgtt tatagaataa gaatatcctc tttaatttgg
     9661 atactcttat tctattttat gttattatat tatcatcctt ttgtttaaaa gtcaattttt
     9721 tctatttttg ttgtttaatt ttatttatac tctttttata tttttcattt tcttttatct
     9781 ttgtaagcac tccatataag ttgacttttc taaatacagt agttataaat accaatccta
     9841 atgcaattga acttgcacat gataatgtac tcaccccata ttctacagct tttatataat
     9901 tattggtcaa aacagaatac atagtattat atattccata accaggtact aatattatta
     9961 aacaacaaac acttaatgta gttgctggag tcatataaat tcttgcacat aactcacaat
    10021 atatcgcaaa acaaactgta gctataaaaa aacataagtt ttctgttact cccatattta
    10081 aaagaaattt atatgaaaac caagctatac ttccacctat tcctgctagt attaagtttt
    10141 ttcctttcat attaaatagc actccaaagc ttaaggcagt aaaaaaagct gctattactt
    10201 ctataaacaa atgcacttat atccccccta acttgaagta taatccaagt accaagaaca
    10261 ttcctactgc tatagataca cctacaaaaa atacttctgc taattttgca agaccagaaa
    10321 tcaactgtcc atctaacaaa tctcttaatg cattagtcaa agcaagcccg ggaactaaga
    10381 gcattattgc tcctgctatt aaatggtcta acgtctttat aaatccgagc tttaaaatta
    10441 aaattgaaca agaagctata ataactgctc caattgcatt tacaaaaaag tcattaagtg
    10501 aagatttact taaatacata ataacaaact tagtcagaat cccaacagta aatgcagcta
    10561 taaattcttc cacatttcct ccaaataata tcgaaaaagt tgctgctgca atacctgcaa
    10621 aaaatattgt tattaaatcc gagtaatagt tatcttcact tatttcatct aaaattttct
    10681 cacataattc aatatctaaa ttatttatac ttattgctct tgataatgag ttaacttcat
    10741 gaaccaagtt taggtctact cttcttgagc ttattctttt aattgctgaa tgtaatttcc
    10801 catctacaaa tacagatgcc attacagctg ttggagttgc aaatacatct acgtggtcta
    10861 ttccaaaaga ttgacatatt ctagctatcg tttcttcaac tctataggtt tctcctccac
    10921 tttgcaacat agctttacct gcatttgagg aaaagtttaa aattctatct atatccacaa
    10981 aacatatctc ccattctttt atagtattat tcatatttta atacaagata ttatttattt
    11041 aaactatttt aaataattca tatccaatat ttacatttta tgaaaaaact tgttttttat
    11101 gcttgtcaaa atcaaatttt aagcttgttt acaccataaa atggctgaat taatatttga
    11161 caaacacttt caaccattta tataaccttc taaattgtat aactttttac tacaaataaa
    11221 attatatctc ttttaattct ttctcttgag tatttaagct tatagcaact tcatatttat
    11281 atatgtacaa tataaaacat tatttttatg atacaaaata taatttgtaa acgtatatat
    11341 gtattaactg tgtatatata aattattctt aaccttatca gttttttcat atatcttcat
    11401 ctatatcgac catatattaa ttacattgac catatactaa taaatgatat tacatagact
    11461 gaagtatatt aaaaaactta aatttagtta tttaacaaca taatactcta atttaatcta
    11521 taaaaatcca tacctaatat aatgcaactc ttattttaac accatctatt acacaaaaca
    11581 acatattaaa aagtaaaata caaaatatcc actataaacc ttattttaat actgaatata
    11641 tattataata tatttaatgt agcttaaata tagtttaaac ataaagaaag gaaatggtat
    11701 atgctaagaa aagataaagt tctattatct ctacataaat tatgtgaaaa tatgacttta
    11761 aaagaccttc aatcagataa aacaggattc actacaatag aaattgctaa ccattcatct
    11821 cttgatagaa gtaataccag taaagaatta aataacctat ataatgaaaa aaaagttctg
    11881 aaaatatctg gtaagccaat tttatttcta catgttagta ttatagaaaa tttaattggc
    11941 cgtactttga acagtgatga attttctata aataattgta atgagctact taaatcaagt
    12001 aaaaatgact cagaagatat tttttcaaca ttacttggac atgattctag tcttaaacta
    12061 cctatagaaa aagctaaatc tgctatactc tatcctccaa aaggtcttaa tacgttactc
    12121 ataggaccta ctggtgttgg aaaaagtaca tttgccgaaa caatgtacaa atatgctgta
    12181 gaaagtaaaa tcttttcttc tgaaagcaaa ttcgtagtat ttaactgtgc tgaatatgca
    12241 gaaaatgcta acttattatt aagtaattta tttggttatg tcaaaggttc ttttactggt
    12301 gctaataagg ataaattcgg catagttgca caggctgatg gaggcatatt attcttagat
    12361 gaaatacaca gattaccacc agaaggtcaa gagatgcttt ttcttttgat ggacaaaggc
    12421 atatacagac gattaggaga atctgacatt gtgcacaaag ctaatgtctt tattgtgtgt
    12481 gccacaacag aagatataca ttcttctctg cttgggactt ttttaagaag aatccctgtt
    12541 acaatcaacc tacctgcttt acaggatagg tctttaaaag aaagattatc tcttattaaa
    12601 tacttcttta ttatagaatc aaaatacacc aatgcaacta taaaagttca caaagatgta
    12661 attaaatcat tactccttta tgattgtttt ggtaatatag gtcagcttaa atctgacata
    12721 caacttatga gtgcaagagc ctttttaaat tacaaaactg gacttaagga taatatagaa
    12781 atagatttat cccttgttcc tgattacatc tatattggtc ttttaaaatt gaatgaaaat
    12841 cgtagtaatc taaataaatt atcagaactt gattatatgc ttttttatga atttaatggt
    12901 aataacttag aagttgaata tacaaaagaa ctatctaaat acaatataga tacatcaaac
    12961 aatggattta aatactctac agagattcct tatctggaaa atgatataag gagtatgttt
    13021 ataaattaca tggaaaagta ctctacaaaa ttactttcga tatcttcagt aactcattct
    13081 gattatacaa gtaatgaaat ttttaaagta atcaatccaa agatttacta tgctgttgaa
    13141 cattctttaa aagaggccga aaaaatatcg agaaacaaat acacaaaaca aacttatatt
    13201 gctctttcaa tgcatataag tgcacttata gaaaatataa atcaagacac ctataatcct
    13261 aagtacaatt tatataatga taaaattttc ttagataaag atttaaatat atctaaaata
    13321 gttttaagta taattgaaaa tgacttacaa acatctttac ctaaagatga agtaaaattt
    13381 atatccatgt ttctaaatag catgtatctt gaaccaaaca atcaatctga aagtatagga
    13441 gtcataatac ttgctcatgg aaattcaaca gcaagcagta tgtgtgatgt agcaaattca
    13501 ttgcttggta caaaccattg taatgcaatt gacatgcctc ttgatgtaaa agtaagtacc
    13561 atacttgata aaactataga gttggtaaaa gaatgtgacg aaggtaaggg tgtacttctt
    13621 ttaacagata tgggctctct tatagctttt ggagaactta tttcagaaaa aacaaaaatc
    13681 aaaactcgct caattgaaat ggtatctact ccaatagttt tagaagcagt aagaaaaagt
    13741 acattaccag aaatgacttt ggatgaatta gctaattcct taagctcttt aaatccatat
    13801 attggacgag cagcaatcga ttcaacagaa ataaactatc ctgaaaagtc aagagagtat
    13861 gtaataataa ctacttgtat aacaggatat ggttcagcta tgaaagtagc agactttata
    13921 aattcttcac ttaaaaatat tcgtaaatac aatattaatt tagttcctca taatatagaa
    13981 agttttaaaa tgtataagaa taaatttagt aatactaaaa ttctggcagt tgttggaagt
    14041 gtagatttta atgtagccaa cacccctttt atacacatag aggaattact aaatggatat
    14101 ggcctagata tgctaaacaa aataatagaa ggtacattca atgccaatag tattacaaat
    14161 ataaaagaaa caaatctatc atacaacagt ttaataataa actttatgac ccaaaactta
    14221 gaacttttgg actctgttaa attattcaac tatgtcaatt cttccttatt tacaataata
    14281 gaattaatag atgaaaactt aaagagcaaa tttaagctag gatatatact acattgctct
    14341 tttatgatag aaagatgttt taaaaataca acttttccat ataataacat tactgaatta
    14401 attagtaaaa atcatgaact atatagttta atacgtgaag cctttaagcc aacagaagct
    14461 tattttaata ttttgatacc agacgaagaa attgcttata taatagacat gattaatgag
    14521 tatctaaata aataaaattt atagcactta ttttgtgcat attaattaat atatgaaatg
    14581 gaggacgaga ttttgaaagg aaaaataaaa cagttaacaa tacttgcatt aatttttata
    14641 tttataactc ctgtatttgc atttgcagat actccaccag taccaaattc ttcaagagca
    14701 gcactactta tagaccagga aactaaaaga atattatttg aaaaaaacat tgatgaaaaa
    14761 atgccattag cgagtttaag taaaatgatg acctttttat tggcaataga agcagttgat
    14821 aagaatcaag taaaagagac tgatatggtg aaaatagaca agtctacagc ttcagtagga
    14881 ggttctactt gtaaacttaa agatggtgat gaaatatctc taggagagct tatgcagggt
    14941 ctaatgctag tatctggaaa tgatgctgct atagccatag ctaaacatat tggtaagact
    15001 gaaaagaatt ttgtaaatat gatgaacaag aaagcagaag aaataggaat gattgatacg
    15061 tattatttta atccaaatgg gcttcctata tatactgacc cagaacataa agaacctcct
    15121 attgaaaata tgtcaactgc tcacgatata gttacacttg gaaagtatat gtatgaccat
    15181 tatgaaaatc aagttacaag aataaccact atgcaagtat ataatgatac taaaaaggat
    15241 tttacacatt ataacacaaa tcccctactt gtttcagtac ctggtgttga tggaataaag
    15301 acaggctata cagataatgc tggatattgc cttgcatttt ctatgatggt acctaaagat
    15361 gctaaaaacg aaagaaatca tagattgata ggtgttgtac ttggtgatgg taacaagaaa
    15421 aatagaattt cttcttccgc tactttattg aaatatggta aagataattt ccattctaaa
    15481 aagatagcac ataagggtga tattatagag actccttgtg ttgatggtat tgatgatttt
    15541 aagataacag ttaaggttga taaagatttg tatggtgttg tttcagataa cgaaaatatc
    15601 aatcctaagg tagtgtttaa aaatatgaat tatcctatcc ataagggaga tattgttggt
    15661 gttgcaaagt attataatga ctctggtaaa tttgttggaa gtgttgatgt aaaaagtgaa
    15721 agtaatatag gatgtattcc tttgaaggat aagattaaga ttaaggttgc taagataaat
    15781 aaaaaacttg agattaaaaa ttctgtttgt tttaaagtat agtaaagcat actattaaaa
    15841 gagtatatta aatttataaa tactccactt tgtggagttt ttttgttttt tacaaaaaat
    15901 taaaatagtg gcaacaattg aggatgtgag ggagagttgc cactattttg tatagggtat
    15961 tatattatgt ttttaagcta gttttactat cttctatgtt tatataataa ctcttgtgtg
    16021 tgaatttaat tgataattaa tatgaaaatt atgtgaagtt ttgatattag gttatacttt
    16081 gaactttatc taacatttta aattttatat ctctaaatta tctaaaatta ctatatcaat
    16141 taactttaca tgataaaaag gctgtgtata aatttatttt taaaaatttt actcacagcc
    16201 ttaaattaaa ttttctattt aaagtatata taaattttat taaataattt gctttttatt
    16261 tcttatttca taaatcctag tattcttgat aacattaatg caattgtagt aggtactttc
    16321 cctgcttcaa atgtagctgt tgctgtgttt aatgctgtta ctgcatcatc tatttctttt
    16381 tgtgttgcat ctgctttatc taatattgct tgtgcttcat ctattgctgt tttatatgt
//
LOCUS       DAEPOZ010000069        16365 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.069, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000069 DAEPOZ010000000
VERSION     DAEPOZ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16365
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.069"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            35..748
                     /gene="ispD"
                     /locus_tag="KR440_001674"
     CDS             35..748
                     /gene="ispD"
                     /locus_tag="KR440_001674"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBH0698729.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDENCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            745..1230
                     /locus_tag="KR440_001675"
     CDS             745..1230
                     /locus_tag="KR440_001675"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBH0698730.1"
                     /translation="MRIGLGYDVHKLTENRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    1325..1533
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1597..3312
                     /locus_tag="KR440_001676"
     CDS             1597..3312
                     /locus_tag="KR440_001676"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBH0698731.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDQCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            3434..4879
                     /locus_tag="KR440_001677"
     CDS             3434..4879
                     /locus_tag="KR440_001677"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBH0698732.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKTKGVAVEVDDRDNYT
                     TGWKFNEWEMKGAPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            5319..6800
                     /locus_tag="KR440_001678"
     CDS             5319..6800
                     /locus_tag="KR440_001678"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBH0698733.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLVEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEITEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            7021..7176
                     /locus_tag="KR440_001679"
     CDS             7021..7176
                     /locus_tag="KR440_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698734.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            7321..8718
                     /locus_tag="KR440_001680"
     CDS             7321..8718
                     /locus_tag="KR440_001680"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBH0698735.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKIFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            8703..9116
                     /locus_tag="KR440_001681"
     CDS             8703..9116
                     /locus_tag="KR440_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBH0698736.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRK
                     VNDLHKLAIKYVKAKAQATIIHEIEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            9223..9318
                     /locus_tag="KR440_001682"
                     /pseudo
     CDS             9223..9318
                     /locus_tag="KR440_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452247.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase (FAD)"
     gene            9624..10454
                     /gene="thyA"
                     /locus_tag="KR440_001683"
     CDS             9624..10454
                     /gene="thyA"
                     /locus_tag="KR440_001683"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBH0698737.1"
                     /translation="MSLADKIFIDMCNNILENGVSSDGEVVRAKWDDGQPAHTIKKFC
                     VVNRYDLSKEFPILTLRPTNLKAAIDELLWIWQKKSNNINDLNSRIWDSWADETGSIG
                     KAYGYQIGQKHKYKEGEFDQIDRVIYDLKYNPYSRRIITNIYNHNDLNEMNLYPCAYS
                     MTFNVTGNRLNAILNQRSNDILVANNWNVTQYAILVHMLAQISDLEVGELVHVIADAH
                     IYDRHIPLVKELINRESYDAPKLIINPNIKNFYDFRVEDFTLENYKSGPQIKKIPVAI
                     "
     gene            10467..10961
                     /locus_tag="KR440_001684"
     CDS             10467..10961
                     /locus_tag="KR440_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBH0698738.1"
                     /translation="MKVIVAVDLNWGIGYGGKLLQSIPEDMKFFKEKTVGNVVVMGRE
                     TFKSLPGKKALKDRVNIVLTRSKSFNDDRLIVCNSIDETLKELQKYENDKIFIIGGET
                     VYKQFLPYCNELYVTKIQKTYEADRFFPNIDIMSNWDLIKESEVKEYNNIRYVFSTYI
                     NNSL"
     gene            10988..12364
                     /locus_tag="KR440_001685"
     CDS             10988..12364
                     /locus_tag="KR440_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBH0698739.1"
                     /translation="MKKDIFVNRVSNALENDRGAVFVGSGISFSSTEVDWFKLLEPLV
                     NELDITLDNKSDDLPMIAQYIVNNYSGNRGPLVNEISKTFNRKFKLNKYHKYLATTKL
                     STIWTTNYDMLLEDAFYDFDTSVKVSDDSITRNRLEYDVEIIKMHGCISRSDWNEIVI
                     TQEDYDDFSINKPAISQRLSEDLINKSFLFIGYSYRDPNIRNIMINARRLSKKHTQQH
                     YIILKKLKDSDSNITQDDVVRQELWCNDLKRLGISTLFIDEYSELEELLREISQKSRG
                     KTVYVTGSHKESNMNARKLGTKLAKHKDITLLSGQSSGIGSEVVAAFTEECIKSKEDI
                     INRMKIFPNPYSADEKYSDDITLIPDLKKCRTKMLNSTQVVVVFSGGIGTEAEIDVAI
                     KQNCNIIPVITCKEDRENPAIKKILNYSDNMKNIEIKDATYYNKIVDEDGVVTMEEIY
                     ICISKIMN"
     gene            12340..13440
                     /locus_tag="KR440_001686"
     CDS             12340..13440
                     /locus_tag="KR440_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBH0698740.1"
                     /translation="MHIKNNELKHDIIKLKKRNGFIEVGKDKKTLVNCLIGANELEDY
                     KYEIKKIDCILNISEKPDIVSDLSTKRRKISESIWYKIVKDTPFVSATLPIYLVSKNN
                     NKIDSNELLDIIIEQMENGVGMITIHPTANKQIFEDSKNRMVPVTSRGGGMVIQDLIS
                     KNFSEDNVYLKILPEIIKYAQKHNVCISLGATFRSGNIFDSNDRAQITEIKSQIELAK
                     RIYKSNVGVIIESPGHSRPSDIIKISSILKKEGFPIMPLGPIPTDIAIGMDHIAAAIG
                     ATIMGIEGCANIISTVTREEHTGGRPSIESTVESIRTAKIAAHIIDIHQMNDINLDMS
                     VSKSRAKSNTCIFGKDTRYCDRCRELCPLCIR"
     gene            13483..13908
                     /locus_tag="KR440_001687"
     CDS             13483..13908
                     /locus_tag="KR440_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HBH0698741.1"
                     /translation="MHRIASFSQQSQRYVKLEQFEYIIPPEIEKIEKAKELFINSMKK
                     YQEDYDKLVEILFENHYNKLIKGGKNEKTSKRQVEKKAIEDARYVFPNACETKMVFTI
                     NARSLFNFFEHRCYENGSCLEGTMTCGDIVQVRKKFKNL"
     gene            13921..14658
                     /gene="rlmB"
                     /locus_tag="KR440_001688"
     CDS             13921..14658
                     /gene="rlmB"
                     /locus_tag="KR440_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBH0698742.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            14661..15188
                     /locus_tag="KR440_001689"
     CDS             14661..15188
                     /locus_tag="KR440_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBH0698743.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            15253..15900
                     /gene="sigH"
                     /locus_tag="KR440_001690"
     CDS             15253..15900
                     /gene="sigH"
                     /locus_tag="KR440_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HBH0698744.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            15974..>16365
                     /locus_tag="KR440_001691"
     CDS             15974..>16365
                     /locus_tag="KR440_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HBH0698745.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVP"
BASE COUNT         6807 a         1676 c         3149 g         4733 t
ORIGIN      
        1 aatgatggtt aaaaataaaa tacgaggtga taccatgtac agtgttataa ttgttgcagc
       61 gggaagcgga aggagaatga acttagatat aaacaaacag ttcataaaat taagagaaaa
      121 agaaattatt gctcatacaa ttcaagtttt ttatgaaaat ataaatatag atgaaatagt
      181 ggtatgtata aaaaaagaag aagaggattt ttttaaagaa aatataataa ataagtataa
      241 ctttaaaaat ataaaaatag catatggcgg aaaagaaaga caggactcaa tatataatgg
      301 tttaaaaaaa ttagatgaaa attgtgatat agtacttatc catgatggag caagaccatt
      361 tgtcgaccat agaataatca acgaatctat aaaggtagct aaagaaaaaa aggctgtggt
      421 tgtaggtgtt ccagtaagtg atactataaa gatagtgtca gatggaacag tacaagaaac
      481 tccagaaaga aatttgttgt gggcagcaca aacccctcaa acctttgaat ataatctcat
      541 aatagatgct tatgagcaag catataagaa caattattat ggtactgatg attcaatgtt
      601 ggtagaaaat ataggtcaaa gtgtcactat ggtaatgggt tcttatgaaa acataaaaat
      661 aactagtcca gaagatttaa atatagcaga acaaatatta aatatggaaa aaagagatga
      721 agttaacagt agaaggagaa ttatatgaga ataggattag gatatgatgt acacaaatta
      781 acagagaata gaaaacttat aatcggtggt gtagagatac cacacgataa aggtttgttg
      841 ggtcattctg atgctgatgt attgatacat gcaataatgg attctatttt aggtgctcta
      901 gcattaggag atattgggaa gcatttccca gatacagatg aagaatataa gggagcagat
      961 agtatgaaat tattagaaca tgtgtataat ctaataacaa gtaaaggata taaaattggt
     1021 aatatagata gtactataat tgctcaaagc cctaagatgg ctccatatat tgagagtatg
     1081 agaagtaata tatcaaaagt tttaaacaca gacattgata atataaacat aaaggcaaca
     1141 acagaagaag gtcttggttt tacaggagca aaacagggaa tagcttcaca aagtatttgt
     1201 ttattattgt tgacaagtca gaataattaa tatattatta gtaataagtt taatggatat
     1261 caagtaatag gcctatatag aatatctata atgtatgaat ttaagtgaaa ttaatataaa
     1321 tataaaatta gctatgataa agaaatagta aaatgatgaa atcttcacag agagaaaacg
     1381 gtggtgagag ttttcaagag gaagtctttg aacctgtctt ttagcttaaa ggtataaaaa
     1441 tctttacggt aggaaccgtt atattcccaa aagagagcta ttcatatagc taactagagt
     1501 ggtaccgcgg aaattaaacc tttcgtctct atacttgtat agagatgaga ggttttttat
     1561 attttcagga agttaatatt caggaggaat ctattaatga aaatgtcaaa aatgtttatg
     1621 ccgacattaa aagaaatccc agcagatgct gagattacaa gccatcaatt aatggttagg
     1681 tcaggcatga taaaaaaaat gacttcaggt gtttataacc aattaccaat ggggttaaga
     1741 gtgttcaaaa aaattgaaca aataattaga gaagaattaa ataaaaaaga ttgtcaagaa
     1801 atactttgtg cagccttact tccttctgaa ttatggaaag agtctggaag gtggactgct
     1861 atgggtgaag aaatgtttag actaaaagac agaacagaaa gagagtactg tcttgggcca
     1921 acacatgaag aagcattcac tgatataata agacaagaaa ttacttcata taaacaattg
     1981 ccactgaact tgtaccaaat acaagtaaaa tatagagacg agagaagacc aagatttggt
     2041 gttatgagaa ctaagacttt tacaatgaag gatgcatata gttttgatgt agatgataaa
     2101 ggtttagata agtcatatca agatatgttt gatgcatatg taagtatatt tgatagatgt
     2161 ggattggaga atagccctgt acaggcagat tcaggagcta taggaggttc tacatcagcc
     2221 gaatttatgg ttaagtcaga agttggtgaa gatgaagttg tattttgtag tggatgtgat
     2281 tatgcagcca atgtagaaag agcagaatct tgtaatctag catcacaaaa agaagaaatg
     2341 aaagaattag aagaggttca tacaccagga gcagctacaa taaaagagtt agaagaattt
     2401 ttaaaaactt ctccagataa gtttgctaaa actttagtat atgaggcaga tggaaaaaca
     2461 gttgtagtcg ttgtaagagg agatagagaa gttaatgaaa tcaaagtatc taatgctata
     2521 ggttctgtta tagaatttgc tcttgctaca gatgatgtag tgagaaaagt aactaatgca
     2581 gaagtaggat ttgcaggtcc aataggtata aatgctgact atgtatttat agataaagaa
     2641 attgttgaac aaagaaacat agttgttggg gctaataaaa ctgagtacca tataaaaaat
     2701 gctaactatg gaagagattt tgaaggaata gttggagatt ttagaaatgt tcaagaaggc
     2761 gaccagtgta tagtatgtgg taagccactt gaaatagcta gaggtgttga agtaggacat
     2821 atatttaaac taggaactaa gtactcagaa tctatgaatg caaacttcat agacaaagat
     2881 ggaaaatcaa agcctatagt aatgggatgt tatggaatag gtgttgaaag aacagcagca
     2941 gctataattg aacaacataa tgatgagaaa ggtataatat ggcctttgtc agtagctcca
     3001 taccatgttg tgatagttcc agcaaatatg aaaaatgaag aacaaatatc aatagctgaa
     3061 aatatatata atgatttgca agctatgggt gtagaagttt tattagatga cagagatgaa
     3121 agaataggtg tcaaattcaa tgattcagaa ttaataggaa tacctatgag gattacagta
     3181 ggtaaaaata ttaatgaagg taaagtagaa tttaaactta gacataaaga agataaagag
     3241 ataattgaca tagaagaaat aaatgaaaaa gtaaaggcag agtttatcag gaataatgtt
     3301 aggttaggat aataacttaa ataagatatt gtatagataa tcaaagttgt actttataga
     3361 caccatttta tctatatata aggatataaa atggtgtttt tttataaaac aaataaatga
     3421 ggaggtaatt gaaatggcaa aaaatgaaaa acaatttgtt gaagaaataa caaagatgga
     3481 agatgatttt ccacaatggt acacagatgt aataactaag actgacttag ttgattatgc
     3541 accagtaaaa ggttttatgg ttgtaaagcc atatggatat gcattatggg aaaagatgca
     3601 agaatttatg gataaaaaat ttaaagaaac tggacataaa aactgttatt ttccattatt
     3661 aataccagag agtttattaa acaaagaagc agaacacgtt gaaggttttg ctccagaagt
     3721 tgcatgggta acacatggag gaaataaaaa attagaagaa agattatgtg taagacctac
     3781 ttcagagact ataatatgta caatgtatgc gaaatggtta aaatcatata gagaattacc
     3841 atatctttat aatcaatggt gctcagttgt tagatgggaa aaatcaacta gacctttctt
     3901 aagaacatct gagtttttat ggcaagaagg tcatacatta catgaaacag cagaagaagc
     3961 acaagaagaa acaatacaac aattagaagt atataaagcg ttatgtgaag aattacttgc
     4021 aatgccagtt gtagctggtc aaaaaagtga aagtgagaaa tttgcaggtg gtgaaagaac
     4081 atatactata gaagctatga tgcatgatgg taaagcatta caatcaggga caagtcactt
     4141 cctaggtcaa cactttacta aggcttttga tattactttt gcagatagag aaggaaattt
     4201 agctaatcca taccatacat cttggggagc atctacaaga cttataggtg gacttataat
     4261 gactcatagt gataacagag gtttagtatt accaccaaga gtagctccaa ttcaagttgt
     4321 tatagttcct atagctgcta aaaaaggaaa tgtaatggag actgttgata aaatatatgc
     4381 tgatttaaaa actaagggag tagccgtaga agttgatgat agagataact atacaactgg
     4441 atggaaattt aatgagtggg aaatgaaagg tgctccagta agagttgaaa ttggaccaaa
     4501 agatatagaa aataatcaag ctatggtatt tagaagagat actttagaaa aggattctat
     4561 gcctttagaa ggattagctg atgctatatg tgatttattt gatgttatac acaatgatat
     4621 gtttgaaaaa gcaagaaaac atagagaaga caatacatct atagttgaaa atatggatga
     4681 gtttagaaaa gctttagaag aaaagccagg attcataaag actatgtggt gtggagatgc
     4741 agaatgtgaa gctaagatta aggaagaaac aggagcaact ataagatgct taccttttga
     4801 acaagaaaac ttaggtcaca agtgtgtgta ctgtggaaaa gaagcagata gtatggtagt
     4861 aatggcaaaa gcttactaaa atacatttaa ataaaaactg gggtagaaag tatttaatac
     4921 tttatcccag tttttttgtt ttttatgagt aataaaatgt atttgtaact atgtcataag
     4981 ataagtcata gatacttata ttaaaaagaa tataatagag aactattttt aataattaca
     5041 tattaatgaa agcaaaatat ttttgttata tagattttca ataaagcaaa caataaatat
     5101 aaatgaatat tagtaaggag tgcttttaat ttttagaaaa taaatgactt aaaagaaaga
     5161 tttatgaaat taaaactagg attttctgat ataataaaca tgtgtatttt taaataaaaa
     5221 ggatattatt attagatttg attaaaaata aacttgcaaa acaatgtact agtttaatat
     5281 tatagttata ttgaataaaa tatcaggagg aagtaaaaat gaatgtaaga gtaaggtttg
     5341 caccaagtcc aacaggattt gtacatatag gtagtttgag aactgcttta tataactatt
     5401 tatttgcaaa aaaaatgggt ggagaatata tattaagagt ggaagataca gaccaaagta
     5461 gattagtaga aggtgctata gagaatatgc ttaatgctat gaaatgggct ggtgtcaacc
     5521 atagtgaagg tgttatatta gatgatagtg gaaaagtagt acaaaaagga gaatatggtc
     5581 catatattca atctcaaaga ttagatatat accaagaata tataaagcaa cttttagata
     5641 gtggaaaagc ttattattgt ttctgtacaa aagaaagact ggatgaagta agagatgctc
     5701 aaagagcagc aggagaaaca gctaaatatg atggtcattg taagaatctt tctaaagaag
     5761 aagtagaagc gaatataaaa gcaggaatac catatgttat aagattaaga ttgccagaga
     5821 atcatacaat taagtttaca gacttagtta gaggagatat ggaatttaat actaatgacc
     5881 tagatgacca agtattaatg aaaacagatg gatttccaac ttatcatttt gcagtagttg
     5941 ttgatgacta tttgatgaaa attacacatg ttataagagg agaagaatgg gtatcatcta
     6001 ctccaaaaca tgtttattta tatgaagctt ttggatggga agcacctgta tttgtgcatt
     6061 taccaaatat acttaataaa gaaaagaaaa aattaagtaa aagacaaggt gatgtagctg
     6121 ttgaagactt taagaaaaaa ggatatttac cagagggatt agtaaactat gtagcattag
     6181 ttggttggtc tcctgaagat aatcaagaat tgtttacaat ggaggaatta gaaaaagcct
     6241 tctctgtaga aagagtatct aaaagtggtg gagtatttga tactgaaaaa ttaaattggg
     6301 ttaatcagca ctatataaaa gatggtgatg atgcttatct aacagactta gcaataccat
     6361 ttttagtaga agatggattt ataacagaag aagaagctac taataaatat gatttcttaa
     6421 agagtatgat atcagtactt aaagagaaat tacaatatgt aaaagaaatt actgaacatg
     6481 caagtatatt ttttggagat aaaatagagg tagaaactga agagggaaga gagttcttaa
     6541 gattagaaca tatacctact ttaatagatg ctttgagaga aaaaatagaa aaaacagagg
     6601 tattaaatgc tgagtttgtt caagctatgc ttaaagaaat acaaaaagag tacaaaataa
     6661 aagggaaaaa tttatttatg ggctcaagaa taattttgac agggcaaatg catggtccag
     6721 atttaccaaa agtaatggaa gttttaggaa aagaaacttg cttaaataga atagcttatg
     6781 taaaaaataa tatattatag tcataaaagt ccgctaattt agaagcggac tttttttgtg
     6841 ttagtatttg gtgaaaatat ctgcttaaga aaatgaaggt atttaaggta ataaatttaa
     6901 tatataaaaa gcaaagcaaa aatataaata aaatatgaat aataaactaa taaaatatta
     6961 agcatagaag aagggaatct attattttag tttgatttaa ttattaaggg ggtaattact
     7021 atgtatagta atattgatga tgttaaaaag gaattaaagg agctatgctt agaatatgtt
     7081 acaattttag aaaaattaaa agatgaaaaa atgattacag aagaaacttt tgaaaagtgc
     7141 tcttctcaaa aaaaaatatt tttagaagaa caatagagga tggtaatagt attattatta
     7201 tgtagtttga aagatagtac gaaaaatgat agtaatacta ttatttattg caattttcaa
     7261 ctataatata atatataatg ttaagaagat aatttataaa aatacagtga ggtgatgagt
     7321 gtgaaagttt ataatacatt gactagaaca aaagaagaat ttgttccatt agaagaagga
     7381 aaggtaaaaa tgtatgtgtg tggtccaaca gtttataact atatacacat aggtaatgca
     7441 agaccattta taatatttga tactctaaga aggtatttag agtatagagg atacgatgta
     7501 acttatgtac aaaactttac agatgttgat gataagatta taaatagaag tcatgaggag
     7561 ggaatatctc ctgaagaagt tgcggctaag tatataaaag aatattttgt agactgtgat
     7621 gggctaggga taaaaagagc tactgttcat cctcaggtta cagataatat acaacagatt
     7681 atagagttta taaaagagtt ggaagataag ggatatgcat atgcagtaaa tggtgatgta
     7741 tattttgata caaataaatt tgaaggttat ggaaaacttt caggtcaaaa acaagaagat
     7801 ttagaggcag gggctagaat tgaggttaat gaccaaaaaa gacatcctat ggactttgtt
     7861 ctttggaagg caaaaaaaga aggtgagcca ggatgggata gcccttgggg agaaggtaga
     7921 ccaggatggc atatagaatg ttctgttatg tctaagagat atttaggtga aacaattgat
     7981 atacatgcag gtgggcaaga tttaacattc ccacatcatg aaaatgaaat agctcaaagt
     8041 gaagctagaa gtggaaaaat attctcaaaa tactggatgc ataatggata tataaatata
     8101 aatgatgaaa agatgagtaa atcaaaaggt aatttcttta cagtaagaga tatatcaaaa
     8161 ttatatgatt tagagatagt aagatttttc atgttatcag cacattatag aaatccagta
     8221 aactttagtg atgagatgtt aaatcaagct aaagcaggtc ttgaaagact atataatact
     8281 aaagaaaaat tagaatttac gctaagtaat ttagtagaat ctcctttaac agaaaaagaa
     8341 gtagagcttg ttaaagaatt agatgacttt agacaaaaat tcatagatgc tatggatgat
     8401 gatgttaata cagcagacgc agtaagtgta atatttgaat tagctaagtt aataaattct
     8461 aatgtagatg aaaattcttc tttggagttt gctaaaaaat gcttagatga atttaatgaa
     8521 cttacaggag tattaaatat tgtaaataag aaaaaggata ctgtattaga taaagacatt
     8581 gaagaattga tacaaaaaag aactgatgca aagaaaaata aagagtttca attagctgat
     8641 gatattagac aacaattact agatatggga attgtattag aagacacaag acaaggtgtt
     8701 aagtggaaaa ggatataaaa atggaaaaaa ctgaattagt tactatgtca ccattagttt
     8761 tagcatactt aggtgataca gtgtatgaga catatataag agaatattta attagacaga
     8821 atacacaaag aaaagttaat gatttgcata aactagctat aaagtatgta aaagctaaag
     8881 ctcaggcaac tataattcat gaaattgaaa ttgagttaac tgaagaagag agtaaaattt
     8941 ataaaagggg aagaaatcaa aagtcaaata cttctcctaa gaatgcagat attatagact
     9001 acaaacatgc aactgggttt gaggcattag tgggatattt gtatttaaat aatgaaatag
     9061 agagacttca atatattatc aataaaggaa tcaaaattat tgaaagagat atgtaaatat
     9121 taaagagtgt tgtcttaata ataagtaata aggcaacact ttttttataa ctaatgtata
     9181 ataaatatat agaaattagt aatgagaaag gataagaaag ttatgaaagt aaaattaata
     9241 tcacatacac ctgaaccaga aaaagtaata gctatggcag ctaagctatg ttattcacca
     9301 gttgggacag acgaaataga aaaagatttc taaaatatat taaagagcat tcctataaca
     9361 aatactatgg cggttggaat ctatgagaag ttatagatat agaattatgt aaattatttg
     9421 gaaaaaacag gtttgaaaat tagccatgtt ggtatttcta ggttacttaa ggaagatcaa
     9481 acataaataa gctgagttgt cttaggtgca ctatgtacaa cattagaatg tgaagttgaa
     9541 gatttattac ttcaaacgaa cgatggtact aagaaaaaca atgatgaaat gattaattaa
     9601 taataaaggt aaggaggtag tatatgagtt tagcagataa aatttttatt gatatgtgta
     9661 ataatattct tgaaaatgga gtatcatcag atggagaggt agttagagca aaatgggatg
     9721 atggccagcc tgctcataca ataaagaagt tttgtgtagt aaataggtat gatctttcaa
     9781 aagaattccc aatacttaca ctaagaccaa caaacttaaa agcagcaata gatgaattac
     9841 tttggatatg gcaaaaaaaa tctaataata taaatgattt gaatagccgt atttgggata
     9901 gctgggctga tgaaacaggt tcaataggga aagcttatgg ataccaaatt ggacagaaac
     9961 ataaatacaa agaaggagaa tttgaccaaa tagatagagt gatttatgac ttgaaatata
    10021 atccttatag taggagaatt ataactaata tatataatca taatgattta aatgaaatga
    10081 atttatatcc ttgtgcatat agtatgacat ttaatgtaac tggaaataga ttgaatgcaa
    10141 tattaaatca aagatcaaat gatattttgg tagctaataa ttggaatgta actcagtatg
    10201 caattttagt tcacatgtta gcacaaataa gtgatttgga agtaggcgaa ttggttcatg
    10261 ttatagcgga tgcacatata tatgatagac atattcctct agttaaagag cttattaata
    10321 gagaatctta tgatgcacca aaattaataa taaatccaaa cataaagaat ttctatgact
    10381 ttagagttga ggactttaca ttagaaaatt ataaaagtgg gccacaaatc aaaaagattc
    10441 ctgtggcaat ttagaaggag ttatttatga aagttatagt agctgtagat ttaaattggg
    10501 gaattggtta tggtggaaag ttactacagt cgattcctga agatatgaaa ttttttaaag
    10561 aaaaaactgt aggtaatgta gttgtaatgg ggagagaaac ctttaaatca ttaccaggaa
    10621 aaaaagcatt aaaagataga gtgaatatag tcttgactag aagtaaatct tttaatgatg
    10681 atagattgat tgtttgtaat tctatagatg aaactcttaa ggaactacaa aaatatgaaa
    10741 atgataaaat ttttataata ggtggagaaa cagtatataa acaattttta ccttattgta
    10801 atgaactata tgtaacaaaa atacagaaga catatgaggc ggatagattt ttccctaata
    10861 tagatattat gtcaaactgg gatttgatta aagaaagtga agtaaaagaa tataacaata
    10921 taagatatgt tttttcaaca tatataaata atagtttata ggtttgtata ggagagaaat
    10981 ttaatttatg aaaaaggata tatttgtaaa tagagtttct aatgctttag aaaatgatag
    11041 aggcgcagtt tttgtaggta gtggtatatc gtttagttcg actgaagttg attggtttaa
    11101 attactggag ccattagtaa atgaacttga tataacatta gacaataaaa gtgatgattt
    11161 acctatgatt gctcagtaca tagtaaataa ttattcagga aatagagggc ctttagtaaa
    11221 cgaaatatca aaaacattta atagaaagtt taaactaaac aagtatcata agtatttagc
    11281 aacaactaaa ctttcaacaa tatggacaac taattatgat atgttgttag aagatgcatt
    11341 ttatgatttt gatacatcgg ttaaagtaag tgatgattct attacaagga atagactaga
    11401 atatgatgtg gaaattataa aaatgcatgg atgtatttct agatccgatt ggaatgaaat
    11461 agttataact caagaagatt atgatgattt ttcaataaat aaaccagcta tatctcaaag
    11521 attaagtgaa gatttaataa ataagtcttt tctatttatt ggatatagct atagagatcc
    11581 aaatataaga aatataatga taaatgctag aaggctaagt aaaaaacata ctcaacaaca
    11641 ttatataatt ttaaaaaagt taaaagatag tgatagtaat attacgcaag atgatgtagt
    11701 aagacaagaa ctttggtgca atgatttaaa acgtttggga atttcaactt tatttataga
    11761 tgaatactca gagttagagg aacttttaag agagataagt caaaaatcaa gaggtaaaac
    11821 agtatatgtg acaggttctc ataaagaaag caatatgaat gcaagaaaat taggaaccaa
    11881 gctagcaaaa cataaagata ttactttact aagtggtcaa tcatctggta ttggctcaga
    11941 ggtagttgct gcgtttactg aagagtgtat taagagtaaa gaagatatta taaatagaat
    12001 gaaaatattt cctaatccat attcagctga tgaaaaatac tctgatgata ttactttaat
    12061 accagattta aagaaatgta gaactaaaat gttgaattca acacaagttg tagttgtatt
    12121 tagtggaggt ataggtactg aagcagagat agatgttgct ataaaacaaa actgtaatat
    12181 aattcctgtt ataacatgta aagaggatag agagaatcca gctataaaaa aaatcttaaa
    12241 ctatagtgat aatatgaaaa atatagaaat aaaagatgct acctattata ataaaatagt
    12301 agatgaagat ggtgttgtaa caatggagga aatttatata tgcatatcaa aaataatgaa
    12361 ttaaaacatg acattattaa attgaaaaaa agaaatggat ttattgaagt tggaaaagat
    12421 aaaaaaacgt tggtaaactg tttaattgga gcgaatgaat tagaggatta caaatatgaa
    12481 ataaaaaaaa tagattgtat acttaatata tcagaaaaac cagatatagt ttcagattta
    12541 agtactaaaa gaagaaaaat aagtgaatct atatggtata agatagttaa agatacacct
    12601 ttcgtatctg ctacactgcc catatactta gttagtaaaa ataataataa aatagatagt
    12661 aatgaattat tagatataat tattgagcag atggaaaatg gagttgggat gataacgatt
    12721 catccaacag caaataaaca aatttttgag gattctaaga atagaatggt acctgtcaca
    12781 tctagaggtg gaggtatggt aatacaagat ttaatctcaa aaaatttttc agaagataat
    12841 gtgtatttga aaatattgcc agaaattata aagtatgctc aaaaacataa tgtttgtatc
    12901 agtctaggtg caacatttag atcaggaaac atatttgatt ctaatgatag agcacagata
    12961 acagaaatta agagtcaaat agaattagct aagaggatat ataaaagtaa tgtcggggtt
    13021 attattgaat caccaggtca ttcaagacca agtgacataa ttaaaatatc gtcaatatta
    13081 aaaaaagaag gtttcccaat tatgccacta ggaccaatac caacagatat tgcaatagga
    13141 atggatcata tcgctgcagc tattggtgct acaattatgg gtatagaagg atgtgcaaat
    13201 ataatatcaa ctgtcacacg agaagaacat actggaggga gaccttctat tgaatccaca
    13261 gttgaatcta taagaacagc aaaaatagct gctcatatta ttgatataca tcaaatgaat
    13321 gatattaact tagatatgag tgtatctaag tctagagcta aatctaacac ctgtatattt
    13381 gggaaagata caagatattg tgacagatgt agagaactgt gtcctttgtg cattagataa
    13441 aaattatatt caagttaatt caataaaaaa gattggataa aaatacatcg tatagctagt
    13501 ttttcacaac agagtcaaag atatgtcaaa ttagagcaat ttgaatacat aattcctcca
    13561 gaaatagaaa aaatagaaaa agcaaaagag ttatttataa attcaatgaa aaaataccaa
    13621 gaggattatg ataaattagt agagatacta tttgaaaatc attataacaa actaataaaa
    13681 ggtggtaaaa acgagaaaac atcaaaaaga caagttgaga agaaagcaat agaagatgct
    13741 agatacgtat ttccaaatgc gtgtgaaaca aagatggtat ttactataaa tgctagaagt
    13801 ttgtttaatt tctttgaaca tagatgttat gaaaatggga gttgtctaga aggaactatg
    13861 acttgtggag atatagttca agttagaaaa aaatttaaga acttgtagga ggtgaataat
    13921 ttggcgagta tagaaggaag aaatccggta atagaagcaa taaaaagtga cagagaaata
    13981 gataaaatat taattgcaaa ttcagctaaa gaaggttcaa ttaagaaaat aataggaatg
    14041 gctaaagata agaatataat tattcagtat gttgataaac ataagctaga tgaagtaagt
    14101 acaagtcatt cacatcaagg tgtaatagct tatgcaagtg agtataagta ttatgaatta
    14161 gatgagctaa tagatttagc aaagaataaa gatgaagacc catttttcat tatattagat
    14221 gagataacag acccacataa tttgggttca ataataagga cagcagatgc agtaggagca
    14281 catggagtta tcattccaaa aagaaggtct gtacacataa ctcctgtagt tgccaaagca
    14341 tctgctggag cagtagaata tatgccagtt tgcaaggtaa ccaatatagt taatacaata
    14401 aaaagactca aagaagaagg gctgtggatt gctgcagctg atatggatgg agaaactttt
    14461 tataaacaaa atcttactgg tcctcttggt gtagtaattg gaagtgaagg ttttggaata
    14521 tctagattgg ttaaacaaaa ctgtgacttt atagtaaaaa tgccaatgat aggtaatgtt
    14581 acatctttaa atgcatctgt agcaggtgga atacttttat atgagatatt cagacaaaga
    14641 ctggataaaa gtaaataggc atgaaaaaaa atataaatca ttacttaata atagatggct
    14701 acaatattat aaatgcgtgg gacaatttga aagaactggc taaagaggac ttagaagatt
    14761 ctcgagaaaa gcttattgat gacattatag aattttctga gtttatgggt tataagacta
    14821 taatagtttt tgatgcttat aatgttaaaa attctcgaga aaaagttgaa aaaagaaaac
    14881 acataacaat agtttataca agagaacacc aaacagcaga tagttatata gagaaattta
    14941 taacttcttt atcaaagtat gatgatgtaa aagtagccac taatgactat gcagaacagc
    15001 aaatgatttt aggaaaaggt gctactagaa tgtctgcaag agaattaaag ttagagttag
    15061 atcgttccaa gaataaaatg aaggaaaaaa atatcggttt gaggaaaaaa attcagcgaa
    15121 attggttgga ggaaaggtta gataaagaaa cattgtcgaa acttgagaac attcgtagaa
    15181 agcgttgaaa ttactaggtt ctttagacta taatatacct ataaatttgt tttatatatg
    15241 ggggagattt acatgttggt agcaaaagaa aaaagttatg agttagtgga caattgtcag
    15301 caggatgagt ataacattgt attaagagca agtgaagggg ataaaatagc actagagtac
    15361 attattacta aatacagaaa ttttgtaaag gcaaaagcaa agtcatattt tttaattgga
    15421 gcagataaag aagatataat acaagaagga atgataggcc tttataaggc agttagagat
    15481 tttgatggaa gtaagaccaa ttcatttaaa tgttttgcgg agatatgtat aacaagacaa
    15541 ataataactg ctataaaaac tgcaacaaga caaaaacata tacctttaaa ttcatatgtt
    15601 tctttgaata aaccaatata tgatgaagag tcagacagaa cacttttaga tattatagct
    15661 acaagtatag taacagatcc agaagagctt ataattagta aagaagagtt aaaaaatata
    15721 gaatcaaaaa tgaacgaatt gttaagtgac ttggaattag aagtactaga attatattta
    15781 aatggaaagt cataccagtt tatagcggac aagcttaaaa gagatgtaaa gtcaatagat
    15841 aacgctttac aaagggttaa gagaaaatta gaaaaacatc ttgaaaacag aaatgattaa
    15901 tagtaaaata cttaccataa gcttattgtg agcaaaatta atttatatcg aaagataagg
    15961 gaggaaattc aaaatggcta aagctaaata cgaaagaaca aaacctcatg ttaatatagg
    16021 gacaatagga cacgtagacc acggtaaaac tacattaaca gcagcaataa caaaaacatt
    16081 atatgacaga tatcaattag gagaagcagt agatttcgca aacatagata aagctccaga
    16141 agaaagagaa agaggaatca caatatcaac agcacacgtt gagtatgaaa caccaaatag
    16201 acactatgca cacgttgact gcccaggaca tgctgactac gttaagaaca tgataacagg
    16261 agcagcacaa atggacggag caatattagt ttgttcagca acagatggac caatgccaca
    16321 aacaagagag catatactat tatcaagaca agttggagta ccata
//
LOCUS       DAEPOZ010000070        16003 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.070, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000070 DAEPOZ010000000
VERSION     DAEPOZ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16003
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.070"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            161..2020
                     /locus_tag="KR440_001692"
     CDS             161..2020
                     /locus_tag="KR440_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0698746.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVC
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICINKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEEILDKEFVYNS"
     gene            complement(2312..2911)
                     /locus_tag="KR440_001693"
     CDS             complement(2312..2911)
                     /locus_tag="KR440_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBH0698747.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(3014..3811)
                     /locus_tag="KR440_001694"
     CDS             complement(3014..3811)
                     /locus_tag="KR440_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBH0698748.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3924..4029)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4121..5107)
                     /locus_tag="KR440_001695"
     CDS             complement(4121..5107)
                     /locus_tag="KR440_001695"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBH0698749.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLNEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            5591..7321
                     /locus_tag="KR440_001696"
     CDS             5591..7321
                     /locus_tag="KR440_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HBH0698750.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            7431..7511
                     /locus_tag="KR440_001697"
     tRNA            7431..7511
                     /locus_tag="KR440_001697"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7465..7467,aa:Leu,seq:tag)
     regulatory      8408..8512
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            8722..11196
                     /gene="gcvPA"
                     /locus_tag="KR440_001698"
     CDS             8722..11196
                     /gene="gcvPA"
                     /locus_tag="KR440_001698"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HBH0698751.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISTLKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHHETLSVLRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYMATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            11196..12653
                     /gene="gcvPB"
                     /locus_tag="KR440_001699"
     CDS             11196..12653
                     /gene="gcvPB"
                     /locus_tag="KR440_001699"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HBH0698752.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIEDMIDQDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPLIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
     gene            13076..15433
                     /locus_tag="KR440_001700"
     CDS             13076..15433
                     /locus_tag="KR440_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBH0698753.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKDDFLYSGSFVVSGEAFVRVENIGKDNYANKIVNDAKISKKHNSQLRNSLN
                     SILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKIRIEVEKYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDNDIYEAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(15519..15836)
                     /locus_tag="KR440_001701"
     CDS             complement(15519..15836)
                     /locus_tag="KR440_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBH0698754.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
BASE COUNT         6035 a         1872 c         2622 g         5474 t
ORIGIN      
        1 ttgtatatat aaaggactct aaagatgaac tatataaaac aaggtttata tattctaata
       61 gtatagaaaa ttttattgta aacgcatgta tattattaga aaaaatagtt tgaaggtatt
      121 caataaacat aaaaaacaat ataggggata aaggtgtttt atgaagagat ttttgaggag
      181 aattatctta gttttattta ttttgttgat ttttataagt tttatttcta ttaaattaat
      241 tcataatgtt ggagattatg gaaaattgat taattatgtt gggattgttt gtggagctag
      301 ccaacgtcta actaaactag agatgaatca tcaaccaaat gatgaattaa tagaatatat
      361 tgatgaaatc ctacaggagt tgattacagg gcatggagat tatggtttag taatcactga
      421 ttgtaatgaa tacaatgagg acttactttt attagaaaaa aagtgggaag atttaaatat
      481 tgaaattaaa aaagtaagaa tgaaagaaca gaataaccag ttattaagta taagtgaaga
      541 gtttttttct cttgcaaatg atacagtgtt taaaatagaa aacttttcta aagaaaaaag
      601 taattactta atgactttaa taataattat atctattata ggtatattag catgtatcat
      661 attgatttta caatatagta aaaaaatggt tagactggaa aaattaaatg tagatttaaa
      721 aaatattgca tataaagatg aactaacagg agtaaatact atagaaaaat ttaaattgga
      781 tgcaaatcaa aatatttgta taaataagga taagaaattt gctgtattct atattgattt
      841 tgaaaacttt aaatacatta atgatatatt tggatatgat tatggtgaca tgatactgaa
      901 aagatatgct aatcttatga tgaatgacat agggaaatat gaaatatttg caagagaaat
      961 tgcagataga tttgttgcgt taagatgcta tgtagataag gaagatttag tagtaagaca
     1021 acgagatgta gacaatgaac taattaatat aacaaatgaa attaaaaata aacatagtat
     1081 tactgttgtc agtggaattt gttgcattga agatgtaaat gaaaaattaa gtatagatgg
     1141 tttaataaat agagcaaatt ttgcacaaaa aactgttaag aataaaccag gtactaatta
     1201 tgctttttat aatgacagta ttcgtaaaaa aatgattgaa gaaaatacta tcaagagtag
     1261 aatacatgaa gctattgaaa aaagagaatt tatagtttat ttacagccta aagttaattt
     1321 acatagtcaa aaaataaatt gtgcagaagc tttagttcgt tggcttactc cggataaagg
     1381 tattatatct ccagcaatat ttattccagt acttgagaag aattttttta ttgccttagt
     1441 agataagtat gtttttgagg aggtttgtaa atggattaga aagaggttag atgaaaataa
     1501 accttttgtt cagatatcag ttaatgtttc aagaatacaa ttttataaca ctaaatttgt
     1561 agaaacttat tccaatatac aaaataaata caggatacct aaaaatacta tagaaattga
     1621 atttacagag agtgttgctt ttgagaatca aaaacatctt ttggaaatta tacatgattt
     1681 gcatgaaaat ggatttactt gttctttaga tgattttgga aaaggttatt cgtctttaag
     1741 tgttttaaaa gatttaccat ttgatgtttt aaagcttgac tctatgtttt tcaaggctag
     1801 tttagataaa gataaagaga aaatagtaat aaaaaatatt gtacatatgt taaaagagct
     1861 taatatcaca actgttgctg agggcattga atgcaaggaa caagttgaat ttcttaggga
     1921 tattgggtgt gatttagttc agggatttgt attttataaa ccaatgccaa tattagaatt
     1981 tgaagaaata ttggataaag agtttgttta taatagctaa aatgtacttt ataaaatatg
     2041 aactaattaa gtaaaattat tttgtactga ataaattttt actttattta cagaatagtg
     2101 attatttaca gaaaagggcg aaaagtgtat tcctttaagt atgggatgga tagcacaaat
     2161 aggggagaaa aaatattggc catataagaa tgcataataa tataaatatt tgaaaggtca
     2221 gcattatata aaaattaaaa agaaaataaa taatttttct ataaaaaaga gactatgaga
     2281 aaagttactc atagtctcct aaataatgta tttattttct tcctgcaacc cattttaagc
     2341 cccaatgaaa ttcagtatcc atttctctat gacctttgaa gtatttaact actttttcaa
     2401 cactgaatgt ctcatcattt acattttcta tcaatgctat tgaacacata ttatagccat
     2461 ttttatgttc atcaagtttg acaactaagt ctgaatcttg tttttgtttg attgttacaa
     2521 ttccatcagc ttcagaccag ttagctacac cttcgtatat aaaagcataa ataagtattc
     2581 tttttatatc ttttatttta ttaccattta ttcttaaatt ttctccttgt gtattacttc
     2641 ctgttctgtc atctccatct aattgagcaa aaggtggatt gtctaaacta ccaaaagcat
     2701 ttcctaatgc ttgaacagca ccttttacac catttttcat ctcaaatagg caacccaaat
     2761 ctaaatctat attactgctt cttaaagagc caaaaaaacc ctttttttgt actttttgat
     2821 tccaatttaa attaactaag atttcaccta aatcgctatt ttcctttttt gtaaggttta
     2881 ttttttgtcc cttttttaat tctatagcca tagtaatctt cctccacaaa catagattta
     2941 tttaaatggt agcacttaat aagtcaatat tcaacaagat tagaataata atattaaaac
     3001 tatactaaat atactaaaaa gtaggaatca caataccttt gtatttttct tcaattaagc
     3061 ttcttgcttt atctgatgta agaacatctt gtagaacttt aatcttttca ctgtctttat
     3121 cttcagttct aaatgctaaa agatttgcat actcgtcatt tataggagct gtataaattc
     3181 cgtttttctt ggcatcaaga ccagcaggta cagcataagt agtatctata aatgcagcag
     3241 taacatctgg aagagaagat ggaatttgag caatatctaa ctctttgaat tgtattccct
     3301 ttggattttc tattatgtcc ttaggactaa ctaatccatc ccctttaggt aacgttaaaa
     3361 gtccaatact ttcaaggtat ctaaggttct tagagcaaga agatgggtta ttgcttattg
     3421 caattgtatc tcctttttta aattcatcaa cagattttat cttataagag taaagtattg
     3481 ctgggcatac atatagtttt ttaccagcca ttattttgta acctttggcc ttaacagctt
     3541 cttttagata aggttcatgt tgaaatagat ttgcatcaat ttcaccatta tttaaagctt
     3601 cgtttggaag tatgtaatca tcaaagtttt taacttctaa agtatatcct ttttctttaa
     3661 taagtggttt taattcttct aataactctc ctcctggaac taaagtagca ccaacaacaa
     3721 tttttttatc atctttagct ttagaacatc caaccacagc taaagttagt actagacata
     3781 ataacaaaca taataatttt ttgaatttca tataaattcc ctcctgaata taattgatta
     3841 gttaaaaata aaataccatt ctttatcaca ctgcaaaata aaaataagcc tgagtttaaa
     3901 ctcaggcttt aaaagctaat ttagactcat ctttcagtat aatactgcag gatttagcac
     3961 caatgtatat aaaaatatac tggttgctgg gtttcatagg gccagtccct tcaccactct
     4021 ggataagaag tatcaatttt taattatgtt ataaatatta tcatgatttt ttacgtctgt
     4081 aaacataatt ttataaatat tttaaaatct ttaatgtatt ttatttaaca cctaaaattc
     4141 cagaaactag aatatcagta tcagcaccta agaaatagtc atttatttca atagaatcca
     4201 gagtatttct caaagcattt tcttcatgcc ttataccaat aagtttattt tctacaaatg
     4261 caacatcaca ttttccaaaa aaatctccaa aaaattttat actagttatt atgcctttat
     4321 caacatttaa attaaactca acatttccac cagaatattt taattcattt gaaagagtat
     4381 attttggtga attaccaaaa ttccaatccc aagtttcgta ttttgattta gtaagctttt
     4441 ctatttgttt tatatcatgc tcattcaaac aataattagt attatcagta tttgttttag
     4501 aaagataatc cattatagca tctttaaatt ctaatatgtc catttttgaa tgtaaatgtt
     4561 cacttatatt ggttacacga gattttacag attttattcc ttttccttca aattttatag
     4621 gtttaacctt taatgctttt gagaggtcat ttatttcaga tgaaaataaa agagtaccat
     4681 gatgcattac tttgtttttg tagttgtatt gagcatttcc agaaaatttc ttgccatcta
     4741 taagaaggtc atttcttcca gaaaattcgg cattgacatc tagtgtttta agtaaatcta
     4801 ttatgggctg agtgaatctt ctgaaatcac taaaattatt attgttacaa gctataaatg
     4861 taaaatttat atttcccaaa tcatgaaata cagctccacc accagactgt ctcctaacga
     4921 caggtataga atattctttt atatattcta aatttatttc agaaagtgtg ttttggtttt
     4981 ttccaacaat tatagaaggt tcattcctcc ataaaataaa taagtcttca tcaaaatttt
     5041 ttagaagata ttcttccatt gctaaattaa aataagggtt tgttttttca ttataaatta
     5101 ataacattaa agtagttcct ccatagaatt gataaaattt ttattatgaa tacaattaat
     5161 tttataattc gacaataata taaaaactcc tttaaattag aaattttaaa aactaccttg
     5221 caaagtttaa ataaataata tatactcaaa atatgtttaa taaaagtgaa tagaagggta
     5281 gaggcgcaat aatctatcag tactaataag gagttaagca cagagagtta ttaggaaagg
     5341 agatattgcc gaagttttat aatcgatgct ttaagattat attgctgggg gtacatagaa
     5401 aatatgtaca actgtcacag gtttatttgt ggtgagctat catttaagga attatattat
     5461 atttattttt actattgttg atatattgta atgtttaagc cttgagtgtt taccactcaa
     5521 ggctttttta gttttattgt aacataatac cctgtacagt tcacaaaatt aaaaggagag
     5581 ggagatttat atgaggaaca aaaaaattaa tattattttt ctgacgacaa tcatgttcat
     5641 aatgtcaaca gtaatggttt ttgcagaaga agatatagac actattgcac tagctaatgc
     5701 agaaaaattt gggattttaa ctttaatacc accattagtt gctataatac ttgcatttat
     5761 aactaaaaac gtaataattt ctttacttat aggtattctt tctggtagtt ttataattaa
     5821 agctagtggt attaatgttt ttgcgacatt tatacaggca tttttagatt tagtagatag
     5881 agctttagta tctttagcag acccttggaa tgcagggata atactacagg ttttggcaat
     5941 tggaggagta ataaatcttg ttgctaaaat gggaggagct aaagctatag ctgaagcact
     6001 tgctaaaaga gcaaagtcag caaaagggac acagcttata acatggtttt tggggctttt
     6061 agtattcttt gatgattatg ccaattcact aatagtaggt cctatgatga gacctgttgc
     6121 agataaaatg aaaatatcaa gagaaaagct agcatttata atagatgcca cagcagcacc
     6181 agttgctggt ttagctataa tatctacatg gataggtctt gaggttggtt taatacatga
     6241 tgcttttgaa agtataagta tagatgtaga tgcatttggt atatttttaa atacaatacc
     6301 atttagattt tacaatatat taatattagc tttcattgta atatcagcat tattattaaa
     6361 agaatttggt ccaatgagaa aagctgagat aaaatctaga agtagaaaaa ttagtataga
     6421 ccttgatgaa ggcgtagaag agttggatga tttagctcca aaaaatggag taaaattgag
     6481 tgtatggaat gctataatcc caataggaac attgataata gttgcattag catcatttta
     6541 ttatagtgga tatacatcta taatgggagg agacgataag gctctgatac aactatttac
     6601 aaattctcca tattcttttg aagcaataaa agaagcattt agtgcatctg atgcatctag
     6661 agcattattt cagtctgcac ttgttgcaag tttagttgct attataatgg ctgtagtaaa
     6721 aaagattttt acaatatcag aagcaattga tgtttggata gatggaatga aaagtttagt
     6781 aatcactggt gtaatattaa tacttgcatg gtcattaagt tcagtaataa aagagttagg
     6841 tacagctaag tttttaatac acctattatc aggttcacta ccaccattct tattaccaag
     6901 tttaatattt ggtttaggag caataatatc ttttgctact ggaacagctt atggaacaat
     6961 gggtatactt atgccacttg caattccatt agcatactca ttaaatcctg atatgtctta
     7021 tgtaatagta agtactagtg cagttttaac aggtgcaata tttggagacc attgttctcc
     7081 aatttcagat acaacaatac tttcttcaat gggagctggc tgtaatcata tagaccatgt
     7141 taacactcaa atgccatatg ctatatttac agcagtaatc actattgtat ttgggtatat
     7201 accagctgga cttggattgc caatatatat agtattacca gtggcaatag ctgctatatt
     7261 tgtaggtatt caaataattg gtaagaaagt tgatgaagcg gaaatagaac ttgtagaata
     7321 aagatataaa taagtttcac cttttattat cactattttg ttagttttta gataatttta
     7381 aaaagttatt gaaaataatg gcgaaatata gtaaaataaa agagtgataa gcggatgtgg
     7441 cggaattggc agacgcacta gacttaggat ctagcgccat tggcgtgggg gttcgactcc
     7501 cttcatccgc actttacagt acttaattgt actgaaaaaa actggggttg ctggctttcg
     7561 gagtgggcta tcccgttttt atttttttgt atatataata gattggcact ttagtataag
     7621 gtgctttttt tataggcaaa aacagtacat ttatttctgt tttattatca ttcatctatt
     7681 agaatatact ttatttaaga atgtgttaat aagcagtgtt cacattcttg ataaaatgtg
     7741 gaataatatg cctaccaata agcatttggt gtataagtaa gagtatttat atagttgatt
     7801 gtgaaatgag tacaacaatt ctgtacagta taattggcat tataattgta gcattgaatt
     7861 taaagtttgc acagtcgctg attggaaaat ttacaaagtg gatgtcaaac atataaattt
     7921 tagtttattg gacagttcaa ggaatcagaa attggaattt tatagtatta atattttata
     7981 gtattaatat aatgtataaa attttatagc aatgtatttc ataagtataa agtgactaga
     8041 attttaagaa atatacaaat tctataaaaa ctaaataatt tcaagtatat tttaaaaatc
     8101 ttatttttca caagtttaaa attgtaaacg atttcatcaa gtctatgaat atacttttaa
     8161 catgttatag taaatctcaa tataaattta catttggttc tttgtgtgtt attttaataa
     8221 taaattaagt gaaaatgtta ttttacatta atatataata tctttgcata agagtgaata
     8281 atataaaaga gaaagaattg ataataaaaa cttaacaaaa aaattaatac ataaaaaaaa
     8341 ataaatataa tgtgttgaca tttaattgaa atattcatac aataaaattg aaaataaata
     8401 tttccaaatg agattagcga gagagtcctt tatacgaaaa ggcgccgaag aagaaattta
     8461 agaaatgcca atttttagag aagctttcag gcaaaaatat cgctaattga tgggactctg
     8521 gagagactta agttaagata ctataagtca ccgaagaagc aaaataatat taaattatta
     8581 aatctttcag gtatacaaac agggatatat aggctaggat tattttccat gctgatatat
     8641 ctttttttta tgaaaaagta caggaaataa tatcaaagtt acttaaatac agtaggtcta
     8701 attttctagg gagggttata tatgaatgaa ttgaaaagag tatctttgta caatatacac
     8761 aaagagcttg gagcaaaact tgttgaattt gctggctggg aaatgccttt agaatatgag
     8821 ggtataaata aagagcatga gaaggttaga aaaagtgcag gtatatttga tgtatctcat
     8881 atgggagagg tacagataaa gggagcagag tcagaaaaat ttatacaaaa cctagttaca
     8941 aatgatataa gcacattaaa gataaatgac attatatata cacctatgtg ttatgaaaat
     9001 ggtggagtag ttgatgattt attaatatat aaatttggtg aagaagatta tttattagta
     9061 ataaatgcag gtaatataga taaagatgta gcatggataa taaaacaaag tgaaggatac
     9121 aatgtagata taaaaaatat atctagtgaa gtgagtcaat tagcaattca aggacctaaa
     9181 gcagaagaaa ttcttcaaaa aatcacagat atagacttaa actcaataaa attttataag
     9241 tcaattccat caacaaaagt atgtggttgt ccttgtttag tttcaagaac aggatatact
     9301 ggggaagatg ggtttgagat atactgtaaa aataaatatg tagaaataat ttggaatgaa
     9361 gttctaaagg ttggaggaga agatatatgt ccagctggtc taggttgtag ggatactttg
     9421 agatttgaag cagctctacc tctatatggt catgaaataa atgaacacat atctccaatt
     9481 gaaggtggtt tatctatatt tgtgaaaaca aataaagaat catttattgg taaatcaatt
     9541 ttatcaaaag aaaaagaaag tggagctaaa agaaagttag taggatttga aatgcaaggt
     9601 aaaggaatgc ctagaaatgg atatgatata aggattggtg ataaaacagt tggatttgta
     9661 acaacaggat gtgcatctcc aacgacagga aaaattttag gtatgggaat tatagattct
     9721 gaatatgcaa aagtaggaaa tgaaataggt atagcaatca gaaaaaaagt tgttcctgct
     9781 gtgatagtta agaaaccttt ttataaaaaa caatataaaa aagataatat tatattaaac
     9841 aaagaaaata aattttcata tatacctgct acaagtgaag ataaatcaaa aatgttaaaa
     9901 gtagttggat taaattcggt tgatgaatta ttttcagata taccagaaga agtaaaatta
     9961 aaaagagatt taaatttgga aattggaaaa tctgaattag aggtaagcaa aatagtaaaa
    10021 agattgtcag aagaaaactt aagtttagaa gacttgactt gttttcttgg tgctggtgca
    10081 tatgaccatt atataccatc aattataaaa catataactt caagatctga attttataca
    10141 gcatacactc catatcaagc tgaaataagt cagggaactt tacaagtagt ttttgaattt
    10201 caatctatga ttgcagaaat tacaggtatg gaaattgcaa atgcatcaat gtatgatgga
    10261 gcaactgctg caatagaagc atgtataatg gcaatgaatc agactagaaa aagtaaaata
    10321 gtagtgtcaa aaactattca tcatgaaact ttgtcagtat taagaactta cttacaatat
    10381 aaagattgtg agatagtaga aattgacttt tgtaatgaat atgggactac agatatagaa
    10441 aaattaaaag cttctgtaga caaggataca gcatgtgttc ttatacaaac tcctaatttc
    10501 tttggaatta ttgaagaaat ggaagaaata gaaaagataa ctcatgaaaa taaggctatg
    10561 ttaattatga gtgtagaccc aatatcatta ggagttttaa aaacaccagg tgagataggt
    10621 gcagatattg ttgttggaga agctcaatca cttggaaacc ctcttaattt tggaggtcct
    10681 tatgtaggat ttttagctag caaatctaaa tatactagaa aaatgccagg aagaatagtt
    10741 gggcaaagtt tagatgtaga aggtaaaatt gcatatgtat taactttaca aactagagaa
    10801 cagcatgtaa gaagagaaaa agcaacttct aatatatgtt caaatcaagc tttaaatgct
    10861 cttgtagctt caatatatat ggctactatg ggtaaagaag gatttaaaga ggttggtatg
    10921 cagtctatga aaaaagcaca ttatacttac aataagcttg tacaaactgg aaaatataag
    10981 ccaatattta aaggtaagtt tttcaaagaa tttgctgtac aaggaaatct taatatagaa
    11041 actataaatg ataagctctt agaagaaaat atattaggtg gttataattt agaatacaat
    11101 tatccagaat taaaaaattc aactttgctt tgtgtgactg agaaaagaag caaagaggaa
    11161 atagataagt tagttggaat aatggaggga ttataatgaa agaatacaat agtttgttga
    11221 tagatatatc taaaaaaggt aggaaagcat actcacttcc aaagttagat atagatgata
    11281 taaaaataga agatatgatt gaccaagata tggcaagaca aagtgagttg aatttgccag
    11341 aagttggtga attagaatta gttagacatt atacattatt atcaaataaa aactttggag
    11401 ttgatacagg tttttatcct ttgggttctt gtactatgaa atataatcct aaaataaatg
    11461 aagatatggc agcacttcca aactttactg gaatgcatcc ataccaatct tcggatacag
    11521 ctcaaggttc tctttctttg atgtatgatt tatcaagaag acttgcagaa attacaggta
    11581 tggatgaagt tacactacag ccttcagcag gttctcatgg tgagtttaca gggcttatga
    11641 ttataaaagc atatcatgaa aatagaggtg accataagag aactaaagtg ataattccag
    11701 attctgcaca tggaacaaat ccagcaagtg cagctatggc aaactttgat gtgattcaaa
    11761 tagcatctga taaaaatgga gctgtagaca taaatgcatt aaaagaggtt ttgaatgatg
    11821 aagtagcagc tcttatgctt actaatccaa gtactcttgg tttatttgaa aaaaatataa
    11881 aagaaatagc tactcttgta catgaagcag gaggactttt gtactatgat ggtgcaaaca
    11941 tgaatgctat tatggggata acaagacctg gagatatggg gtttgatgtg gttcatctta
    12001 atcttcataa gactttttca actcctcatg gaggtggagg tccaggagct ggtcctgttg
    12061 gagttaaaaa agaattagta ccttttttac caataccatt aattgaaaga aaagaagata
    12121 agtatgtact aaattatgat agagaaaaat ctattgggaa aattaaaaat ttctatggta
    12181 attttggagt tcttgtaaga gcttacacat atatactaac tatgggaagg gatggactta
    12241 aagaggctag tgaaatggca gttttaaatg ctaattatat aaaggaaagt ataaaagatg
    12301 attatatttt gccaatagat accttgtgta agcatgagtt tgtattaggt ggattgccaa
    12361 gaggagaggc caatataaaa actatcgata ttgcaaaaag actactagat tatggatacc
    12421 atcctccaac aatgtacttt cctcttatta taaatgaggc acttatgata gaacctacag
    12481 aaagtgagag tatagagaca ttagatagtt ttatagaagc tatgaaaagt gttgctaaag
    12541 aggcaaagga agaaccagaa ctattgaaaa ctgctccaca taatactttg gtcaaaagag
    12601 tagatgatgc aagggcagta aagaaaccta tactaacatg gtcacaaaga taaataatta
    12661 aaaagtagag atttaaagta taaagagaat taaatttgta agaaaaagct atctaactta
    12721 atccagttta gatagctttt ttgtatatta gtatgtatgg tattttctat ataaagtttt
    12781 aaattatatt agtgcttata tagaatattg atatgatgat tatgtataga tattaataag
    12841 aattgttgaa tttatcttgc cttaacttta tttactcttt tatcttaaga aaaacttaac
    12901 taaaatatag cgaaattcat aaaaatttag gaaatcttaa gaattataat atttaaatct
    12961 ttagatttat attttattat ataaactata gaataataaa atataaaaat tgagaatttt
    13021 ataatatatt tgtagatttg agtataaaaa agtgctaagt tgaaaggagt aaaatatgga
    13081 taaggttaat ttagaaaggt atagttgtga tataaatgtt ggtttaaata aaaatcaact
    13141 tcaaagtaga atagatgata atttaattaa tgcatttgat aatggaaaaa ccaaaacata
    13201 taaacaaatt tttaaagaca atatatttac actttttaat ctcataaata ttgttttagt
    13261 ctgtttatta gtttcagttg gttcttttaa aaatacctta tttatattta ttgctgtaat
    13321 taatactatt ataggagtaa tacaagagat aagagctaaa agattattgg acaatctatc
    13381 tgttatagtt tcaaataagg tatctgtcat aagagaaaaa gagaaaaaag agattgatgt
    13441 acaagaatta gtattagatg atattatgat tttaaaaaca ggtaatcaaa tttgtgcaga
    13501 ttcaaaagtt ttaaatggaa aattagaggt aaatgagtct ttggttacag gtgaatctga
    13561 tattattata aaaaataaag acgatttttt atattctgga agttttgtag tttctggaga
    13621 agcttttgta agagttgaaa atataggaaa agataactat gcaaataaga tagttaatga
    13681 tgcaaagatt tctaaaaaac ataattctca acttagaaat tcattaaatt caatattaaa
    13741 aattgtaggt ataataataa ttcctttagg aattatattg tttgcaaaac agcattttgg
    13801 aaatggagat tctatagcta catctgtagt aggtacagtg gcagctgtaa atggtatgat
    13861 accagaggga ttaacattac ttacgagcat tgctctagct gtaggaacta taaaacttgc
    13921 tagtcataaa actttagtgc aagaactgta ctgtgtagag actcttgcta gagttgatac
    13981 actatgtctt gataagacag gtacaattac agaaggtaaa atgcaagttg atgatatagt
    14041 aatgttggaa gaagtggata ttaatgaaat aatggggaat atatgtaatt ctctcaaaga
    14101 tgataatgca acactaaatg caataagaga taagtataat gttttagata cgtataaagc
    14161 taagaattta ataccatttt cttcagaaag aaaatacagt ggggttacct ttgaaaataa
    14221 aggtacatat ttattaggtg catttgagtt tatatttaag gaaaaaaata aaaaaattag
    14281 aatagaagtt gaaaaatatt ctaaaaaagg aaacagagta atagttttag ctcatagtga
    14341 ttcttatatg gaagataata atattcctga agatataaag ccactagctt ttatattgat
    14401 attagataaa attagagatg aagctaagga aactttagaa tttttttaca atgaaggtgt
    14461 agatataaaa attatatctg gtgacaatcc tataacagta agtgaggtag ctagaaaagc
    14521 aggtcttaaa acggctagta attttattga tgcaacaaca cttaaaaatg ataacgatat
    14581 atacgaagca gttgataaat acagtgtttt tggaagggta agcccacaac agaaaaaaca
    14641 aatgatttta gcattaaaaa aacaaaatca tacagtagct atgactggag atggtgtaaa
    14701 tgatgtgcta gcgcttaaag aagcagattg cagcattgcc atggcatctg gaagtgatgt
    14761 tactaagaat gtttctaatt tagtcttatt agattcaaac tttgcatcaa tgccaaaggt
    14821 tgttatggaa ggacgaaagg taattaataa cattcaaaga gcatcttctt tgtttttagt
    14881 aaagacaatt ttttcattct tcttgtcact attaacatta ttcttcttca gtagacagta
    14941 tccatttgtt cctatacaat tatcactaat tggagctgta acagtgggta taccatcatt
    15001 cttcttagct cttgaagcaa acaaaagtag aatttcagga aactttttac tcaatatatt
    15061 aaaaaatgct ttaccaggag cactatgtgt gacagtaaat gtaattattg tttacaatat
    15121 agttgaatat ttaggctata gttcaagtgt attttctaca atgtgtgcta tattaactgg
    15181 tgtatgtggt cttttaatat taagacaaca atgtttgcca tttaataaag aaagattatt
    15241 tttaattgta agtatgacta ttgcatttgt tattgggatt ctatttttag gtggattatt
    15301 ctcatttgta gcattgagta gagaattaat tctcataact atggcattag caatattaat
    15361 gccaattatg ataaaagtta tgttatttgt cttggatgag atattagaga caattgttcc
    15421 agatttaaat taattttaaa atgatataaa taagagcagt tttagttgaa ctgctctttt
    15481 tagatgtgtt taattttata taagaatatg tatttttatt aatcagctat atatgagcaa
    15541 cagtagtcaa caacttcatt aacctttaaa tcaaaacttg aatcactagg aacattaaat
    15601 ttttgccctt ctttataagt tacaaactca ttgcttccag gcagttttac atccatactt
    15661 ccagcaagca tctccattat ttcttcttct ctagtagaaa aagtatattc tcctggcaac
    15721 attaatccta aagtttttct ttctccatta ggcagtatta ttactctgct acttacctta
    15781 ccatcaaaat aaacgtttgc cttctttact gcagtaacat tcttaaattc actcattaga
    15841 taaaacctcc aaaattattt gtacatatat attatataaa aataatgtaa aaaatgctat
    15901 atatttgcaa aaaaaaattt agtatttatt aaaaaaatta ataagaataa aaaagagatg
    15961 aaattgctta aattgataaa aaatataaaa taaattgcaa agt
//
LOCUS       DAEPOZ010000071        15971 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.071, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000071 DAEPOZ010000000
VERSION     DAEPOZ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15971
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.071"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(420..959)
                     /locus_tag="KR440_001702"
     CDS             complement(420..959)
                     /locus_tag="KR440_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HBH0698755.1"
                     /translation="MQYAIELYYDKKTEKQLFDLSKKIADEKISTKYLEWKTRPHLTL
                     ACFNDVDEACCIDKLKGFAQNHKVIPAYIGSVGMFNDTKTVFVSPIMNSSMYQFQREL
                     YEVLQDFDSNGWEWYHPNRWVPHCTIALTGDDESEAFFRASDVLLREFKKISGEFVSI
                     GLVKITFPVEEIFTVNMNR"
     gene            complement(1036..2841)
                     /locus_tag="KR440_001703"
     CDS             complement(1036..2841)
                     /locus_tag="KR440_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0698756.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIYKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNVCKNKDINIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            complement(3249..3626)
                     /locus_tag="KR440_001704"
     CDS             complement(3249..3626)
                     /locus_tag="KR440_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBH0698757.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            4477..4962
                     /locus_tag="KR440_001705"
     CDS             4477..4962
                     /locus_tag="KR440_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698758.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFDLDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            complement(5162..5701)
                     /locus_tag="KR440_001706"
     CDS             complement(5162..5701)
                     /locus_tag="KR440_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH0698759.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEASCSQTDDMGEILEKMIGADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVAAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(5694..6107)
                     /locus_tag="KR440_001707"
     CDS             complement(5694..6107)
                     /locus_tag="KR440_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0698760.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            complement(6143..7264)
                     /locus_tag="KR440_001708"
     CDS             complement(6143..7264)
                     /locus_tag="KR440_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBH0698761.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILVPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            7863..9515
                     /locus_tag="KR440_001709"
     CDS             7863..9515
                     /locus_tag="KR440_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HBH0698762.1"
                     /translation="MNNKNLAILISGMMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYKMSTNVLKEIDKIKKVENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSDILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAVPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKIEYEITINAITGKILEFEIDD"
     gene            9812..10561
                     /locus_tag="KR440_001710"
     CDS             9812..10561
                     /locus_tag="KR440_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0698763.1"
                     /translation="MNTILKVNNITKTYNKGQQPSLDKVSFNVSEGEFIGIMGASGSG
                     KTTLLNVLSTIDKPTSGDIFINNINLKSLNDTCAADFRKDNLGFIFQEYFLLDSLTIF
                     ENISVPLTLQKLNPDKIENMVKSLAERFDIASQLNKYPNELSGGQRQRASAVRAIVKH
                     PSILFADEPTGALDSNSATELLHSLQEANEELKTTILMVTHDAYAASFANRILIFKDG
                     HIIKELNKQPNNRHTFYELIIQEIAKLDTKR"
     gene            10574..12601
                     /locus_tag="KR440_001711"
     CDS             10574..12601
                     /locus_tag="KR440_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBH0698764.1"
                     /translation="MNIFYMAIKNLKKNFSFYSLYLLSVSFVITVFFAFTSFSVNKIM
                     LEKISTDGRVESMCNVISIFLMAFVIFYMAYSNRFFLRRRTKELGIYALLGYRKFTIL
                     SLLTFENVLICSGAFITGLVLGALSHKGIVFGITALLNLSIDNSQIPFFNINAINKTI
                     IFIALVVLVLAISNGKFLYKTSLVKLIRHEKSAEKKLKFRKIPAFMGFIMILSGYCLS
                     LDILRGAKSVWVSIGFYQMGLITMIIIVIGTVLFINSFLPYIMQKSKKNKRTFYTDMK
                     IIAIPNFIYRIRSNSKTLIMLTLLSATTLTVSSVMALSLYYPIASVSRIAPSEIEFRV
                     EKNKQIEDVKQIVSQYTSNTDDIIFTQTNIYKVKSSSKDLPFEYEIGTSKNNLSNSSS
                     SKEVISRESGFECISFSDYYTLLKAQNRTKLLKQLTPLRDNECILMKYQPNNDGYSEN
                     GTIYPLLINGKETPVAVRETSLNNAISFANSVGTLIVNDSIYEQIAQNKLPMTSILSI
                     NGKSIKNNENLYSNISDYLDGSPYLQGYSHRINDLLSINSSTFLLIGFLVVLFFIAVG
                     SILYFNNISAISDSTSDYKILQKMGYDNIQIKKIIKKQVLPFFNIPFLLGILDCIFAT
                     LVYKTALMQNLLGNSFSQYIPVIIAIFLTSVVYLIYYILTIKSCCKIVLKN"
     gene            12745..14328
                     /locus_tag="KR440_001712"
     CDS             12745..14328
                     /locus_tag="KR440_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0698765.1"
                     /translation="MTNFNLKILFLGNYILSLLFMGISTSTETYMNIQSIIPLIISTL
                     LLIDITYITSCNVRENNIISLFCGLLALDSWYLLLSFVTTPIGTIIFKILSTVIVYVS
                     IKFIFLFLFQGSGYNFRKITDILLLITCIGAIVGAFISNRTYACMYGIQFVVSIISFL
                     YIIFYHLKRVVFVIKSEKKPISISVFITIIGFLTYYFFTLRISNHIGNFGIYLTVLVF
                     FMSIHGIALKENDSFPISTVFNIKQRAIFLFISILVISLVVLFANGNFSIILIAINVL
                     FMLIFLCNIILEFNLRDEKNKVVKNSKYTFALEKLQQEEQLKTEFSNFLHDDILQNLL
                     SIKNMMSKSYRPEVQDIIYETLNNLNTCIRNQMQDYHPVILKNLTVKENYQNLIESVS
                     SSFPQKNICVSFKCSDTLFLVEPYDILIYRLIKELLTNVYKHSNGSHAWIALALEKNI
                     IKLNINDNGVKNTSFKTPLDITKITFDIIKHKGIISIKEQVENLGGTISISDNIPHGV
                     CVQITIPMKGDGSYKYFVS"
     gene            14300..14920
                     /locus_tag="KR440_001713"
     CDS             14300..14920
                     /locus_tag="KR440_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0698766.1"
                     /translation="MVLINILLVDDHMLFAKSLSIALEEYEEIEQFYSTQDIHNLSEM
                     IQDKKIDIVLMDINLRGLSECDGLKVAYNLLETNSTIKIIILTGYDLPVYKHEAKKIG
                     IYGFLNKNISPDSLIDSLLKVYQGVTCFPSENTEVIIEDLTNMEKRILQLICSGKKRK
                     IIADELYISERTLSNHLQHIYDKLDVSSSVEAITKAIKMGYISPVY"
     gene            complement(15108..>15971)
                     /locus_tag="KR440_001714"
     CDS             complement(15108..>15971)
                     /locus_tag="KR440_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBH0698767.1"
                     /translation="NRIRFNHYVMRKENIQRLKGTLPRGQFMMTVRKAASDLDVSIST
                     ISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSENSNAEIVTKNSDIFKNIEKYIDA
                     TINNNQKTMKYKHRDELFKNSNTNTYSRINQESIDKDYDLIIHTGYETENETKKKELL
                     KNNLNKKNLNKNTFENLDEDLTEDSTYKNPESIHEIIIRKLNSESGKNFKADLSITKA
                     LIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEPTILFGYEFESYLNEESSLSAC
                     NYGDMCYEDMY"
BASE COUNT         5638 a         2412 c         1964 g         5957 t
ORIGIN      
        1 attgggaaat ttttatcaat acgagaatta tcaattcgag aaatcgttta tttattttaa
       61 ttcttccttt attatgctct tattttttat atgtctattg ttttgtcaga tatattacta
      121 cttttatttt ctataaatat atggctataa ccttcttacg cataaactct ataaatttat
      181 atactttata ttctttatta cccaaatatt ctttattacc caaagcctcc aaaatggctt
      241 aaatcttttc atagcttctt acagaagttt acatgggcgt ccctccaata agcaaaagtg
      301 caatatgtgt accattgtgt cctacttgcg actattgagc tgtcgaaatt cgcaacttcg
      361 catttatctg atgaaatgca ctatgcaaaa tattcataac atccacaaca gagttaaagt
      421 tacctattca tattcactgt aaaaatttct tccacaggaa atgtaatttt aaccaatcct
      481 attgagacaa actcaccact tatttttttg aactcacgca gtaatacatc acttgcccga
      541 aaaaaagcct cactttcatc atctccagta agtgctattg tgcaatgggg tacccaacga
      601 ttggggtggt accattccca accatttgaa tcaaaatctt gtagtacctc atataattct
      661 ctctgaaact gatacatact gctattcatt ataggtgata caaacaccgt tttagtatca
      721 ttaaacatgc ctactgaacc aatgtaagca ggtattacct tatggttttg tgcaaacccc
      781 tttagcttgt ctatgcaaca agcctcatcc acatcattga aacaagccaa agtaagatga
      841 ggtcttgttt tccattctaa gtacttcgta ctaatttttt catcagcgat ttttttagat
      901 aaatcaaaaa gttgcttttc cgttttttta tcataatata gctctatcgc atattgcata
      961 ataacacctc caccataaag taaaaatacc tatctataaa aacacacaaa tacctatcat
     1021 tttaatatta tattactact gttcctcttc caattttcta tatagtgtag ccaaacctat
     1081 acctaaatcc tttgctatct tcttcttcgt ctttgtatct cttccatact tattcaacaa
     1141 cttgtttatg taagtcaact ctaattcttt aattgaaagt atcctttcct gattctctac
     1201 tatccctcca actatgtcat cttcaaatat tatatttata tccttatttt tacaaacatt
     1261 tccattaata ttataataat ttaaaatatc caatggaagc atatctttat atattattcc
     1321 atcatctcca accaaattca tcatatattc aactgcattt tccagttccc ttatatttcc
     1381 cggccaatta tagttaatca acatattcat tacattttct tctattttat ttaactgaat
     1441 acctaattct aaagaatatt tagaagcaaa cttcatcatt attgcctcaa tatcatcctt
     1501 tctttcccta agaggtggca aatttatagg aattacattt aatctatagt ataaatcatc
     1561 cctaaactta ccttcattta ctaatttcaa taagttttta tttgtagcag caatcactct
     1621 tatatctaaa tctataagtt tatttgaccc aattctcgca aattttctct cttgtaaaac
     1681 tctaagtaac tttgcttgta aaggcattga caaatctcct atttcatcaa gaaatataac
     1741 accttcattt gcaagttcaa atttacctac tttacctcca ctactagctc cactaaatgc
     1801 accttttata tagccaaata gctcactttc aaggagattt tctggaatag cagcacagtt
     1861 tatcgcaata aatggcttgt tccatctgct accttcagca tgtatagccc tagcaattag
     1921 ctctttaccc gtaccacttt ccccagttat aagtacagtt gaatttgact ttgctaattt
     1981 cttagttctc tcttttactt ttaacattgc ctctgaattt ccaattatag actctatatc
     2041 actatttccc cagccactat tgacttttcc atgtccttta tgattgatat atgtcttgtc
     2101 aaatattatt attttgtcgt attgatttat atatggatat accggaatta ttttggcatt
     2161 tatattataa attttgttat caatatcaat ttctagtaat tcatgtccat ttgaggaaga
     2221 ttcaacagat actatattta ctatattttc tattatattt ttatatatat ctaatttttt
     2281 tagagctata ttgtttatat aagatatttt gctatttatg tctagtatta taacgccttt
     2341 ttcaacattt tcaatgattt ctatcatcat atccattttt ctactgctat tttttgacgc
     2401 ttcatgttct tctattttca tagaaattaa atcgcatata tgattgacaa attttaaata
     2461 attatctatt tttgcactta tttctacttt tttagtttta tctgtactaa tagcaccaat
     2521 tactcctata gttcgattat tatactttat tggaactgct attattactt tatttaggca
     2581 tttcatgtaa tgggaacatt ctatacaaag ctgattatca catatatcca atacgactaa
     2641 ttcctgacct gtctgtataa cattgtcgta aataaatcct tctgtaacaa cgctttcatt
     2701 tatctttgat ttatacagac ctgtaccact aattcttatt aatctatcat caactatttc
     2761 aatatctaca tctaacacac tagctactgt ttcagtgtat tttatcattt cttgttgaaa
     2821 ttcttttaat ttacttaaca tagtttcccc cattagaatt taatttacca aattatcagt
     2881 ttagctatat tttaacatat agtcactctt taaaatatat ataaaacatt acttatttat
     2941 atatttacat taaaacacac aatattaatt tataaaaagt ttgttttata tgatgacctt
     3001 aattacttaa ttataagata cagacaataa agtaaatcac aatacaataa taaaataaaa
     3061 aaagctttcc tcatataaca aatatatttt tgacataaaa atatacatta tatgagaaaa
     3121 gctcaatagt taaatattta gatagatttt tatatagact tttttaatat aaactttttt
     3181 aattgtagac tttttttatt agtttttaat ttttattagt tttgttattg gttttattgt
     3241 tataatactt ataatactgc cattgcttca atttctaaaa ctgcatcttt tggtaattga
     3301 gcaacttgta cgcatgacct tgctggtgga ttttctttaa aatatgtagc atatacttca
     3361 ttaatatcat taaaatcctt taaatctttt acgaatactg tagtcttaaa tattttatcc
     3421 atacttgtac cagcttgttc taatattgct tttacattat ctaaagaagc ttttgttgct
     3481 tcttttgggt cagatactaa ctcgccagtc ttcatatcta ttggaagttg acctgatgta
     3541 gaaactatgt ttccaacttg aattgcttga gaatatggtc caattgctcc tggagctttc
     3601 tctgtagcta ttactacttt tgacatgact tcacctccta ttattaagtt atatttacag
     3661 gaaaataaaa tttcctaaac accaatatta atacgaacct ttagattaaa tttcaaatct
     3721 aaaacttcaa ataatcaata cttagatgat tatgtttatt tgtgtcttgt taacttttat
     3781 taaagcaatt aacgtgccaa actttattta tatataaaat tttgtgaagt cattcaaaat
     3841 agttacttct gaattttttc gtaaaaaaat gctttaaaat tactttttta tgatgtcaat
     3901 ttttttctca tatctagaaa tttaccaaca tgagaattct tgttttgaga atttacagat
     3961 taaagttatt tttcaatctt aaattatcaa gttattacat atgaaagaac tatagttgac
     4021 aatcccacta ttataaataa taaaaccttt acccatacct tatttaccct agttatcata
     4081 gagcctattg aaaatataac agctaaatat aataataacc aaaaaattat caatatctac
     4141 cactgttgct gaaaatccaa aaaactacct tttgatagta ttacttaaca atctaattaa
     4201 tctcataata aatttacaac ttataaataa attgttatat tggccgtatc tcaacaactt
     4261 tctcctttcc tagttttaaa tcttttatag aattgtacca cgtctaagta aattttcata
     4321 ctattaagtc ttatttctaa taaatcatta gctctcattc tatgcccaat aatgtaagat
     4381 tgtagtaatt ttattgattt taatagtttt atttaatata cttatattaa ttattttcat
     4441 gtgaaattat tacttatata gggggatttt tttaatatga gcaaaagtga attaacggca
     4501 gaaacaacag aagaaatgtt agaagtacta agtggtaaag attatgatat tgcatgtcat
     4561 ttacatgaac ttggtaaatc attagattgt aaaattgaac caaaaacagg tgctcgttct
     4621 tacaaaatag tatattcaac taagaaacca aaacgtagct tatttactat tgaatgtaat
     4681 gaaaaaaaat ggagagttaa agcaaatctt tttcatctaa atacatataa agatgctgtg
     4741 gaggaatgct ctaaaactat taaagatagt ataactaaaa ctcgtacttg taagaaatgt
     4801 aattcaaagt gtattggagg ttcttcgttt gatttagatg gaaagtctta tctgacttgc
     4861 ataggaagtg gtcattattt tgcaaatatg gaagaaatgg attggaaaaa cctagaaaaa
     4921 ttaattacta aagaaaataa tattatgcag gaatctgtat agttttttaa gggtaattat
     4981 taaaatatat gagtaacaac agatacttaa ataggcataa aaaaataaag ggtataataa
     5041 ggatatagaa taaaaataaa tgatatagca caaagaggct gtctcaaaat atagataagc
     5101 ctctttttat tgttgaaatg ataagaatat attcgatttt atacaatttt atacaaacat
     5161 actaagccaa cttacccatt tcatatgcct gtttcattgc cttacttccc ttaatatcac
     5221 ctacattcca agcacctaca ccatatacta tacctttttc tttagaacca tttagacatg
     5281 atgtaaatcc tctaaatcct tctattgttc tttccatatc agccacattt tcatctgctg
     5341 ctgcaacgat aaaatagaaa tctttatcac aaatttcagt atatctagag caagttctat
     5401 caatcatagt tttcatttgc gcattcatag tataaaaata aacgggagtc gctaaaacaa
     5461 tcacatctgc cccaatcatt ttttcaagaa tttctcccat gtcatctgtc tgtgaacaac
     5521 ttgcttcttt ttcataacaa gcaccacatc cagtacaata gttaatatct ttatctttta
     5581 aaaatatctt ctccacttga tttttagatt ctaatacccc ttgtataaac tggtcacaca
     5641 ataagtcaga atttccacct tttctaggac tagctgatac tattaataca tttttactca
     5701 taagatactg cctccatttt cttatttcta ctgctcttta atactttttc tttctcaata
     5761 ataccttctt catatctatc aatcttaaaa tttaatcgtt ccaaagtttt ttgcatttct
     5821 tctattcttt ttgctagttg gtttctctgc tctattaaaa gttcttttct agctttgata
     5881 gtactgctcc cttgttgaaa catagctaca tactcaatta atgtctcaat agaaagacct
     5941 gcacctctca tacactttat gaaattaacc cactcacaat cttcttctgt aaaagaccta
     6001 attccacttt tatttctatt tacaggtggt attaaaccaa ttcgttcata atatcgtaaa
     6061 gtatccgctg aaagctcgta ttttttacta acttctgaaa tcgtcataca tatcttctcc
     6121 tcttgatatt gtatagattg aattatttct caaaggtttc tgctacattc tttaaagcat
     6181 ctataatttt aatgtgttga ggacagcttt cttcacattg tttacattca atacaatctg
     6241 aggcctttcc ataagtttta gtataattat tatagtaaac catctgtgtt gaaaatccag
     6301 tattaagagc ttgtttttct gcattgtaca gcgcaaaata gtttggaata gcgatatttt
     6361 ttggacagcc ttctacacaa tactgacagg cagtacaagg aactaaaatt gattcgttga
     6421 taatctctac tgcttcatct ataatattat attcctcttg tacgaatggt ttgaaatttt
     6481 gcatatatcc tgtattgtct aataattgtt ccatatttga catcccactt aagaccatca
     6541 taacattatc tttacttgct gcaaaacgta ttgcccaaga tgaaattgac atatctggat
     6601 tgtagtcatt caataatttc tctgcttttt ctggtatctt tgcaagtgta cctcctttta
     6661 ctggctccat aacaattaca ggcttattat gcttctctgc cacctcgtag cattttctag
     6721 attgtatgct ttcattatcc caatctaaat aattaatctg taattgcacg aagtctactt
     6781 ctggatgctc actcaagact ttatccaata gttctgcact atcatgaaaa gaaaaaccaa
     6841 tattctttat ttttccttct tttttctttt cttcaataaa cttaaagcta tcaaattttt
     6901 tagctacttc ataatgtgac acgcctatat tgtgtaaaag atagtagtca aaatagtcta
     6961 caccacattt ttctaattgc tcattaaaaa tggtttcttg ctcttctttt ttctttaaag
     7021 ccattaatgg aagttttgta gctatagtaa aactctctct tttgtgtctt tttactagag
     7081 attctctaag tgctatctca ctttttccca tgtgatacac ataggctgta tcaaaatatg
     7141 taaatcccct ttctaaaaaa gtgtctacca ttttatttaa atgttccata tcaatagttg
     7201 taacatcatt ttcatctttt attggtaaac gcattaatcc aaatcctaat ttttttgcct
     7261 ccattgtttt acttccccct ttaaaacttg tttgtttttt ctctctactt taactattct
     7321 tacaagtata taattcttgt ataattatac tttactactt ggagttaact tcaagtcaat
     7381 agattttttt atttttaaga caaatattat aaaaatacag ttctttaata ttaatccatt
     7441 gcatgtttct ttcaccctcc aattctcaaa cttcacaaac tccaacatgc aaagaaatat
     7501 atactttaag aaattcagtc cattaatatt actacaaata ctttttagat atgtttaaaa
     7561 agaaatttca cctaaaatat ttataaaaga tttagaaata ggtcttgtgt ctattttaaa
     7621 atatataaaa cagtggtaaa gacttattta taagataaaa aatgaatagt gtataccctt
     7681 tttaaaaagt tttttccttt ttatatttgt gatacaatgt taaatataaa aaggaaaatt
     7741 ttgtattaaa gaaatcttat ttgttgattt tgtaacttca aactcagtat caatttgatt
     7801 ttaagattat attttattaa gtatgctaaa taacaaattt ttaaagattg tgaggtaaaa
     7861 atatgaataa taaaaattta gctatattaa tatcaggtat gatgtttttt tctagttctt
     7921 cagcaatttt tgcagacaat actacaaagc aagaaagatt aataggtaaa acaagatatg
     7981 aaacagcagt agaagtaagt aaattgggat gggttcaatc aaaaacagcc attatagtta
     8041 atggtaattc tattcaaagt gcgctttgtg caaatccatt tgctaagcta aagaatgcac
     8101 ctatactttt agtaaacaac aacagtattg aaaattcaac taaggctgag cttaaaaggc
     8161 ttggtgtaga taatgtgtac attgtagata gtggaaatag catttcttct aaagtagaaa
     8221 atgaaataaa atcacttaac ataaaaataa ataaaatagt aggaaacaat atatataaaa
     8281 tgtctacaaa tgtacttaaa gaaatagata agataaaaaa ggtagaaaat gttgcagttg
     8341 taaatggact aaagggatta tctgatgcag ttagtatagc ttctccagca gcaatacata
     8401 atatgcctat aatattagta tcagatgaaa acagcaatga tagtagtata aatttcttaa
     8461 acaataggtc tataaaaaaa tcttacatag ttggaggaaa taagatttta tcagacaata
     8521 cagtaaagaa atatccaaac agagaaagaa tcagtggttc agatagaaac aatactaatg
     8581 caaaaattat tgataaattt tataaagata aaaaaataaa taatgtattt gtagctaaag
     8641 atggtattaa ccaagaaaat gcattagtag attcattatc aataggagta ttagctgcta
     8701 aagaaaattc tcctataatg atatctaatg gtagccttgt aaatagtcaa aaggaattta
     8761 ttaaaaataa agatatagat gaaataacac aagtaggagg aggatataat caaaaagcat
     8821 ttgaagatat tttaaaacta aaaaatgtga acataagaaa taaaactaaa aaaatatctt
     8881 taatacaggc taaagaaata ttaaaacaag ctcttccagg aagcgatatt ttagtttgtt
     8941 attatgatga agatgataat gaatatgact gcaaagttat aaaggataat atcctatatg
     9001 agatagaaat tgatgcctac tctggtaaaa taatagaaat tgaaaaagat gatgacttaa
     9061 atgataataa tttaggtaca caaaatatat cattggataa agctaagtct atagcattag
     9121 atgctgttcc taatggtaat ttaatctatt gtaaatatga tgctgaagat aacgaatatg
     9181 aggctaaagt aataaaaaat aagaaaacat atgaattaga gataagtgca tttgatggaa
     9241 gaattgtaga tattgacatg gacaatgatt ctgatgatga ctttgatgat aatgataaga
     9301 ataactctaa tactaatgac aacagttcta ttattagtgc tgaaaaagct aaatcaataa
     9361 tgctagataa agtgcctggt gggaaaatag taaaatttaa gtatgatgaa gatgacaaag
     9421 aatatgaagg tgaaataata aaaggaaaaa ttgaatatga gataacaatt aatgcaatta
     9481 ctggtaaaat attagaattt gaaatagatg attaataaaa aatatatatt ctattaatta
     9541 ttacaagcaa taaagccata aatattgaat ttttcaatat ttatggcttt attttatttt
     9601 ttttaattta aatctacatt atgccattat atatctatgc actcttttaa attaaaatct
     9661 tgtccatcat cccaaaccta ataaagttag attttaaaca atatattgtg atataagtaa
     9721 tttgtgtaga attattgcgt gatttactca tgaagaaaca ataaggttaa tatataattt
     9781 gactatatta agacgaaagg agacatatac aatgaataca atattaaaag taaataacat
     9841 aactaaaact tataataaag ggcaacagcc gagtttagat aaggtatcct ttaatgtatc
     9901 agaaggtgaa tttataggta taatgggagc ttctggctct gggaaaacaa ctctgcttaa
     9961 tgtattatct acaattgata aaccaacaag tggagatatt ttcataaata atataaattt
    10021 gaaatcgctt aatgacactt gtgctgctga ttttcgtaag gataatttag gctttatctt
    10081 tcaagagtat tttttactag atagtcttac aattttcgag aatatttcag tacctcttac
    10141 acttcagaaa ctcaatccag ataaaataga aaacatggta aagagtctag ctgaacgatt
    10201 tgacatagct tctcaattaa ataaataccc aaatgaatta tctggtgggc aaaggcagag
    10261 agcttcagca gttagagcaa tagtaaaaca tcctagtatt ctctttgcag atgaaccaac
    10321 aggagctttg gattctaatt cagctactga actactccac tcactacagg aagcaaatga
    10381 ggagttgaaa acaacaattt taatggtaac acatgatgcc tatgcagcaa gttttgcaaa
    10441 tagaattttg atttttaaag atggtcatat cattaaagaa ttaaataaac aaccgaataa
    10501 tagacataca ttttatgagt tgattattca agaaattgca aaattggata ctaaaagata
    10561 ggaggataat ttaatgaaca ttttttatat ggcaataaag aatcttaaaa aaaacttttc
    10621 attctattct ctatatctac tttcagtctc ttttgttatt actgtatttt tcgcatttac
    10681 ttcattctct gttaataaga taatgctaga aaaaatttca actgatggta gagttgaatc
    10741 tatgtgtaat gttatttcga tatttttgat ggcatttgtc atcttctata tggcatactc
    10801 aaatcgattc ttcttgagac gtcgtacaaa agaattaggc atatatgctt tacttggcta
    10861 tcgtaagttt acaatattat cgctacttac ctttgaaaat gtattaattt gtagtggagc
    10921 ttttattact ggattggtat taggagcatt gtcacataag ggtattgtct ttggaattac
    10981 agcactactt aatttatcaa ttgataattc acagatacca ttttttaata taaatgcaat
    11041 aaacaaaaca attatcttta tagcattagt tgttttagtc ttagctatat caaatggtaa
    11101 atttctttat aagacttcac ttgtaaaatt gataagacat gaaaaaagtg cagaaaaaaa
    11161 attaaagttc agaaaaattc cagcctttat gggatttatt atgattttaa gtgggtattg
    11221 tttatcacta gatattcttc gtggagctaa atcagtatgg gtaagtattg gattttatca
    11281 aatgggcttg ataactatga ttatcattgt aattggtaca gttctcttta tcaattcctt
    11341 tcttccatat attatgcaaa agagtaaaaa aaataaacgt actttctaca cagatatgaa
    11401 gataatagct attccaaact ttatctatcg tatacgttca aactctaaaa ctctaattat
    11461 gcttacatta ctctctgcta ctactttgac agtttcaagt gttatggcac tgtccttata
    11521 ctaccctatt gcatctgttt cgcgtattgc accttcagag atagaattta gagtagaaaa
    11581 aaataaacaa atagaagatg taaaacaaat tgtcagtcaa tatacgtcta atacagatga
    11641 tattattttt acacagacaa atatttataa ggtaaaatct tcatcaaaag accttccatt
    11701 tgaatatgaa attggtacat ctaagaataa tttatctaat agtagttctt ctaaagaagt
    11761 tatttcaaga gaatcaggat ttgaatgtat atctttttct gattattata ctttattaaa
    11821 ggcacaaaat cgaacaaagc tactaaaaca acttacccct ttaagggata atgaatgtat
    11881 tttaatgaaa tatcaaccaa ataatgatgg atatagtgaa aatggaacga tttatccatt
    11941 acttattaat ggtaaagaaa cacctgtagc tgtaagggaa acatcattaa acaatgctat
    12001 ttcttttgcc aatagcgttg gaacacttat cgttaatgat agtatatatg agcaaatagc
    12061 acaaaataaa ttaccaatga caagcatttt aagtattaat ggtaaatcaa ttaaaaataa
    12121 tgaaaattta tatagtaata tttcagatta tttagatgga agcccttatt tacaaggata
    12181 ttctcacagg ataaatgatt tattatcaat taatagttct acatttctgt taatcggatt
    12241 tcttgtggta ttattcttta ttgctgttgg aagtatttta tactttaaca atatatcagc
    12301 aatttcagat tcaacatctg attataagat tttacagaaa atgggttatg ataatataca
    12361 aattaaaaaa attattaaaa aacaagtatt gccatttttt aatattccgt ttttacttgg
    12421 tatattggac tgtatatttg ctaccttggt ttataagact gctttgatgc agaatttact
    12481 tggaaattca ttttcacaat acatacctgt gattattgca attttcttaa cttctgtagt
    12541 gtatttgata tattatatac ttactattaa atcctgttgt aaaattgtat taaaaaatta
    12601 atatttacca aatcgtcaag cttagaggtg tgacgatttg gtatacttca tttataagta
    12661 tttgatataa tttaaaatta tagattctta ggtttgattt agtcataaaa ttaatataat
    12721 taactcataa ggaggtgtat aagattgaca aattttaatt taaaaatact atttttagga
    12781 aactacattc tatcacttct cttcatggga atatctacaa gcactgaaac ttatatgaat
    12841 atccaaagca ttattccctt aataatttca acattattac tcatagacat cacatatata
    12901 acctcttgta atgtccgaga aaataacatt atttcattat tttgtggatt actagcacta
    12961 gacagttggt atttactctt atcatttgta accacaccaa taggcactat tatttttaaa
    13021 atattaagca cagttatagt gtatgtctct attaaattta tatttttatt tttgtttcaa
    13081 ggtagtggat acaatttccg taagataaca gatattttat tgcttatcac ctgcattggt
    13141 gcaattgtag gagcatttat ttctaacaga acgtatgctt gtatgtacgg aatacagttt
    13201 gttgttagta taataagctt tttatatatt attttttatc atttgaaaag ggttgttttt
    13261 gtcataaaaa gcgaaaaaaa gcctatatcg atttcagtat ttataacaat tataggattc
    13321 cttacttatt atttttttac actaagaata tcaaatcata ttggtaattt tggaatctat
    13381 ctaactgtat tggtattttt tatgagtatt catggtattg cacttaaaga aaatgatagt
    13441 tttcctattt caacagtttt taacataaaa caaagggcta tatttctttt tataagtatt
    13501 ttagtaatta gcttggtcgt actatttgcc aatgggaatt tttctataat attaatagct
    13561 ataaatgttt tatttatgct tattttcctc tgtaacatta ttttggagtt taaccttaga
    13621 gatgaaaaaa ataaagtagt taaaaatagc aagtatactt ttgcattaga aaaattacaa
    13681 caagaagaac aactaaaaac agagttttct aattttttac atgatgacat attacagaac
    13741 ctgctttcaa taaaaaacat gatgagtaag tcatatcgcc cagaagttca agatattata
    13801 tatgaaacac ttaacaatct aaatacatgt ataagaaatc aaatgcaaga ctatcatcct
    13861 gttattttaa aaaatttaac agtaaaagaa aattatcaga atttaattga aagtgtttct
    13921 tcatcctttc cacaaaaaaa catttgtgtt tcatttaagt gttctgatac tttattctta
    13981 gttgaaccat atgatatatt gatatatcga ctgattaaag aattattgac aaacgtgtat
    14041 aaacattcaa atggtagcca tgcatggata gctttagcac tagaaaaaaa tataataaaa
    14101 ttaaatatta atgataatgg tgtgaaaaat acatctttta aaacaccttt agatataact
    14161 aaaattactt ttgatataat aaaacacaaa gggattatat caattaaaga acaagtagaa
    14221 aatctaggag gaacgataag tatttctgat aatatacctc atggagtttg tgtacagatt
    14281 acaatcccta tgaaaggaga tggttcttat aaatattttg ttagttgatg accatatgct
    14341 ttttgctaaa agcttgtcaa ttgcactaga agaatacgaa gaaatcgaac agttttattc
    14401 cacacaggac atccataatc tttcagaaat gattcaagat aaaaaaatag acattgtctt
    14461 aatggatata aaccttagag gcttaagtga atgtgatggc ttaaaagtag cttataattt
    14521 attagagact aattcaacta ttaaaataat aatcttgact ggatacgacc tcccagttta
    14581 taaacatgag gctaaaaaaa taggtatcta tggattccta aataagaata ttagccctga
    14641 tagtttaatt gattcattat taaaagtcta tcaaggggta acctgttttc catctgaaaa
    14701 tactgaagta attattgaag atttaactaa tatggagaaa agaattttac agcttatctg
    14761 tagtggtaaa aaaagaaaaa taattgctga tgaattgtat ataagtgaaa gaacattatc
    14821 aaatcattta caacacatat atgacaagct agatgtttca tcatcagttg aggctattac
    14881 aaaagccatt aaaatgggtt atatttctcc agtatattaa aaaagtgagt gtctcaaaat
    14941 gaactttcta gctcataagg cactctcctt tgtattaaat caaatatatt ttcatcattt
    15001 aaacaattaa gaagaggctt ctctctattt tgagacaact ccattttaat acactattta
    15061 tatttatata taaacatatt ttaaacaact aaaaagcaac taatatctca atacatatct
    15121 tcataacaca tatcaccata attacaagca gataaagaac tctcctcatt taaatacgac
    15181 tcaaattcat acccaaacag aattgttggt tctatatata tttccatatc tgtatatctc
    15241 catcttctta cctttgcctc tataaccttg taaaaatctt ctaaactata cccttctttt
    15301 aatcttttat ctataagtgc cttagttata ctcaaatcag ctttaaaatt ttttcctgat
    15361 tcactattta attttcttat aataatttca tgtatgcttt ctggattttt ataagtagaa
    15421 tcttcagtca aatcttcatc caaattttca aaggtatttt tatttaaatt ctttttattt
    15481 aaattatttt ttaataattc ttttttcttt gtctcatttt ctgtttcata cccagtatga
    15541 attatcaaat cataatcttt gtctatactc tcttgattta tacggctgta tgtgttggta
    15601 ttactgttct tgaacaactc atctctatgt ttatatttca ttgttttttg gttattgtta
    15661 atagttgcat caatgtattt ctctatattt ttgaaaatat cactattctt tgttacaatt
    15721 tctgcattac tattttcaga acaaacataa ctatatacag aacaacaatt tccctttact
    15781 cccctactta ttaatctcaa tatacctaaa tctataaatt catttacaag tctacttatt
    15841 gtagatatag atacatcaag gtcagatgca gctttcctta ctgtcatcat aaattgtcct
    15901 cttggcagag ttccttttaa tctttgtata ttttcttttc tcattacata gtgattaaac
    15961 cttattctat t
//
LOCUS       DAEPOZ010000072        15959 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.072, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000072 DAEPOZ010000000
VERSION     DAEPOZ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15959
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.072"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            235..384
                     /gene="rpmG"
                     /locus_tag="KR440_001715"
     CDS             235..384
                     /gene="rpmG"
                     /locus_tag="KR440_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBH0698768.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            410..631
                     /gene="secE"
                     /locus_tag="KR440_001716"
     CDS             410..631
                     /gene="secE"
                     /locus_tag="KR440_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBH0698769.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            651..1193
                     /gene="nusG"
                     /locus_tag="KR440_001717"
     CDS             651..1193
                     /gene="nusG"
                     /locus_tag="KR440_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HBH0698770.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDVDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1229..1654
                     /gene="rplK"
                     /locus_tag="KR440_001718"
     CDS             1229..1654
                     /gene="rplK"
                     /locus_tag="KR440_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBH0698771.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            1724..2422
                     /gene="rplA"
                     /locus_tag="KR440_001719"
     CDS             1724..2422
                     /gene="rplA"
                     /locus_tag="KR440_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBH0698772.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    2474..2602
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2645..3151
                     /locus_tag="KR440_001720"
     CDS             2645..3151
                     /locus_tag="KR440_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBH0698773.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3209..3574
                     /gene="rplL"
                     /locus_tag="KR440_001721"
     CDS             3209..3574
                     /gene="rplL"
                     /locus_tag="KR440_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBH0698774.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            3966..4754
                     /locus_tag="KR440_001722"
     CDS             3966..4754
                     /locus_tag="KR440_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBH0698775.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            5225..8941
                     /locus_tag="KR440_001723"
     CDS             5225..8941
                     /locus_tag="KR440_001723"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBH0698776.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGQSFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     VEATEDEDIENLEEFTEDDLFEEEIDFESDDFDM"
     gene            8983..12468
                     /gene="rpoC"
                     /locus_tag="KR440_001724"
     CDS             8983..12468
                     /gene="rpoC"
                     /locus_tag="KR440_001724"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBH0698777.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVLVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            12757..13179
                     /gene="rpsL"
                     /locus_tag="KR440_001725"
     CDS             12757..13179
                     /gene="rpsL"
                     /locus_tag="KR440_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBH0698778.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            13302..13772
                     /gene="rpsG"
                     /locus_tag="KR440_001726"
     CDS             13302..13772
                     /gene="rpsG"
                     /locus_tag="KR440_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBH0698779.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            13821..15887
                     /gene="fusA"
                     /locus_tag="KR440_001727"
     CDS             13821..15887
                     /gene="fusA"
                     /locus_tag="KR440_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBH0698780.1"
                     /translation="MARKFPLEKTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFINKMDILGADFYNVISMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         6246 a         1985 c         3249 g         4479 t
ORIGIN      
        1 atggattaaa aggaataaca aaaagagggg ttaggctcct ctttttttaa catagaaata
       61 caaaaacatt gacacagcat aggattttgt gataacttat acaaggtaat gattatagga
      121 agttaataat atcttgaata ctgtagattt ttaataaata catattttaa agatattata
      181 ttcacatata ttaacaatat atattattgt attacaaagt tggaggtgta tcagatgaga
      241 gttaaagtaa ctttagcatg tacagagtgt aagcaaagaa actacaacac tactaagaat
      301 aagaaaaata atccagacag aatagaatta caaaaatatt gtagattctg taaaaagcat
      361 acaactcata aagaaacaaa atagtttttt ataaggatgt gagtatggaa tggctgccca
      421 aaaaaatgac ggtgcaaaaa ctaaaaaaag gtttagttta tttggatatt taaaagaaac
      481 taagcaagaa ttaaaaagag ttacatggcc gacaaaaaag gaactgttta aaaacacaag
      541 tatagtttta actgtagtta tttcatgcac tatattagta tggggtatag acactatatt
      601 atctggtgca ctagcgttat tactaaaata gacgaaaaga ggtaagttaa atgtcagaat
      661 tacaagaagc aagttggtat gtagttcata cctattcggg acatgaaaat aaagttaaag
      721 ctacaattga gaaagcagtt aaaacaagag gtatggaaga ctgcataaga caagtagttg
      781 ttccaacaga agaggtagtg gaaactacta aaactggaaa agaaaaaact agacaacgta
      841 aagtttatcc aagttatgtc ttggttaaaa tgataataac tgatgaatct tggtatgtag
      901 taagaaatac taagggagtt acaggtttcg ttggacctgg ttcaaaacca gtaccattaa
      961 gtgaagatga agtcaaagct atggggatag atacaacaga ccctaaagta gtaaatagcg
     1021 atgttgattt tgaaatagga gacacagtaa aagtatctca aggaccattt agtggacaaa
     1081 taggtaacat agaagaaatt gacttagaaa atagagaagt caaagtatgc ataaatgcat
     1141 ttggtaaaag gactctattt gtaatagagc ttgaaggtat agaaaaaatc taatactata
     1201 tagaaaaatc tgaggaggtg cgctcaaaat ggctaaaaaa gttataggtc aaataaaatt
     1261 acaaatacct gcaggaaagg ctactccagc gccaccagtt ggaccagcat taggacaaca
     1321 tggtgttaat ataatgggat tcacaaaaga atttaatgct aaaactgcag atcaagctgg
     1381 aatgataata ccagttgtta taactgtata tcaagataga tcattcagtt ttataacaaa
     1441 aactccacca gctgcagttt taattaaaaa agcattaaac ttaaaatcag gttctggaga
     1501 accaaacaaa aagaaagttg ctaaaatgac ttcagctcaa gtaagagaaa tagctgagtt
     1561 aaaaatgcct gacttaaatg ctgcatcagt agaagctgct atgagtatga tagctggtac
     1621 tgctagaagc atgggtgttg taatcgaaga ctaattaaaa gatacacttt ttttataaag
     1681 tgggaggcga gaagccgaaa ttaaccacaa ggaggaaaat aaaatggcta aaaaaggtaa
     1741 aagatatgca ggtgcattac aaaaagtaga tagaactaaa ttttatgatg catcagaagc
     1801 attaacatta gtttcagata tagctggagc taaatttgat gagacagtag aagcacacat
     1861 taaattaggt gttgactcaa gacatgctga ccaacaagta agaggtgcag ttgtattacc
     1921 tcatggaact ggtaaaacaa aaagagtttt agtttttgct aaaggtgaaa aagctaaaga
     1981 agctgaacaa gctggtgctg actttgtagg agctgaagaa ttagttcaaa aaatacaagg
     2041 tgaaaactgg tttgactttg atatagtagt tgctactcca gatatgatgg gtgtagtagg
     2101 tagattaggt agagtattag gacctaaagg tttaatgcca aaccctaaat caggaacagt
     2161 tacatttgat gtagctaaag ctatagatga aataaaagct ggtaaagttg aatacagatt
     2221 agacaaaact aacataatac acgttccagt aggaaaagta tcatttggtg gagaaaaatt
     2281 aactgaaaac tttactgcat taatggatgc tataataaaa gctaaaccag ctgctgctaa
     2341 aggacaatat ttaagaagta taacagtagc ttcttcaatg ggacctggag ttaaaataaa
     2401 cccagcaaga acagctgaat aataaaactg ttgacacagg ttgacaaaaa tgttattata
     2461 tcacttgtca atgtaaaaaa gaatagattt gccgtagata gtgggtgcga aagcttaata
     2521 tcccgccgag gttttagata aattgagatt actaaagctt ttgatgtcta cttttggcaa
     2581 caaaagcttt ttataatgtt gcggtaaata ttaagcctat ttggtgaagg aggtgcacat
     2641 ctatatgaga aaagctatag aaattaaatc agaagttgtt tctgagatag ttgaaaagtt
     2701 acaaaaatct tctgctgctg ttgttgtaga ttacaaagga ttaacagttg aagaagtaac
     2761 tgagttaaga aaacaaatga gagaagctgg tgtagattac aaagtataca aaaatacttt
     2821 agttagaaga gcagctaaag aagttggtat agagcaattt aatgatgaat tattagttgg
     2881 aactaatgcc atagcatttg gatatgatga tccagttgct ccagctagaa tattaaaagg
     2941 attcatggat tctcatccaa aaatgaaatt aaagatgggt attgtggaag gtgcattcta
     3001 tgatgaatct aagattgttg aaatggctaa cataccatca agagaagttc ttatcgctaa
     3061 attacttggt agcttaaaag ctccagtatc aaacttcgca tacttaatag atgcaatagc
     3121 gaaaaaggca gaaggtcaag aagaagctta attgcttaac taaaaaatta taaatattaa
     3181 tttgaaataa aattcggagg tgctaaaaat gacaatagaa caaatattag aagctataga
     3241 aaatatgaaa gttttagaat taaatgaatt agttaaagct gctgaagaaa aattcggtgt
     3301 atcagcttca gctccagtaa tggtagctgg tgcagctgct ggtggaccag ctgctgaaga
     3361 aaaaactgag tttgatgtag tattaactga cgttggttct tcaaaagttg gagttataaa
     3421 agcagttaga gaaataacag gattaggatt aaaagaagct aaagaagtag ttgacaatgc
     3481 tcctaagaca gttaaagaag gagcttctaa agaagaagct gatcaaataa aagagaaatt
     3541 agaagctgct ggagctaaag tagaagttaa gtagtttctt taaaaagata cccattgggt
     3601 atcttttttt atttataaaa aactataaag aaagtatttg gaattataaa gaaagtgttt
     3661 ttataaaata aaaaaaattt atataatagt actaaattaa gggtatcata tatggtgtaa
     3721 tggtatttta atttaacatt attatcaaat taaaatataa acaattgttg taatgtctgt
     3781 atataaagta tataattaaa ctaaaatgta aaattttaca ttttagttta aaaaaataca
     3841 cgagtgtttt ataaataaaa tgtaataaat aattaattaa caaatataaa ttttaaaata
     3901 agttagatat ttagaaaaat cgaaatttgt aggatttaaa ggacatattc aaggaggagt
     3961 taataatgga aaaattacaa ggaaaaattg cagtagttac tgcagcaaca aaaggtattg
     4021 gattagcatc agcagagata ttagcaaaaa atggagcaac tgtgtactta gcagctcgtt
     4081 cagaagaatt agctaatgaa gttataaata aaataagtgc agaaggtggt tgtgctaagt
     4141 ttgtttactt taatgctcgt gaagaagaga cctttacctc aatgatagaa gaggtagtta
     4201 aaaaagaagg taagatagat atacttgtaa acaattttgg ttcaacaaac ccttctcttg
     4261 acaaagacct tgtaactggg gatacagata atttctttga cacagtaaat actaatttaa
     4321 aaagtgtgta tttaccatgt aaagcagcaa ttcctcatat gataaagaat ggaaaaggaa
     4381 gtatagtaaa tatatcaagt ataggttcag tattacctga tttatctaga atagcttact
     4441 gtgtatcaaa agcagcaatc aactcattaa ctcaaaacat agctacacaa tatgctaaag
     4501 acaatgttag atgtaatgct gtacttccag ggcttattgc aactaaagca gcattagaca
     4561 atatgtctcc agagttcata aaagaatttt taaagcatgt tcctttaaat cgtatagggg
     4621 aaccagatga tatagcaaaa gctgttctat tctatgctag tgatgactca tcatttataa
     4681 caggggattt acttgaagtt gcaggaggat ttggtttacc tactccacaa tttgcagata
     4741 atatattagg ataattaaat tactaatatg caatgaaagc ttatgcatta gttatagtag
     4801 aaatgatatt tttataaagg ccatagatat aatttatatt ggaaaggttt gaggttcaaa
     4861 attccaagtt aaatttattt atggtcttta ttttgtatat tttaataaat tgtatatatt
     4921 tgtataaatg taattattta caatcatttt aatatggaat tataattaaa taggggggat
     4981 ggtacttgta taaaattttt tatagaaaat tagacctata aatgaaaaat atggatttta
     5041 tattggaaaa ttgtacataa tacataatat atgcttaaaa taatttacaa aaaatcaaaa
     5101 ataatgcata gtttacatat aagataaaaa atatattgaa aaatacttga cattatgcta
     5161 aaattattag atgcgttaga atgaactatt gttttatttt taattattga gaggtgaaaa
     5221 gtgaatgcca catcctgtca cgataggtaa aagaactaga atgagctttt ctaaaattaa
     5281 ggaaatagct gatgtgccaa atcttataga aattcaagta gattcctatg agtggttttt
     5341 aaaagaaggt ttaaaagaag tgtttgatga tatttcacca atagaagatt atactggtaa
     5401 tcttatattg gaatttgtcg actattctct agatgataaa ccaaagtacg atatagaaga
     5461 gtgtaaagag agagatgcta catattgtgc acctctaaag gttaaggtaa gacttataaa
     5521 taaagaaaca ggtgagataa aagaacaaga agtgtttatg ggtgacttcc ctttaatgac
     5581 agaaagagga actttcgtta taaatggagc agaaagagtt atagtaagtc aattagttag
     5641 atctcctggt gtttactatg cggaagaaag agataaaaca ggtaagagat tgatttcatc
     5701 tactgtaatt cctaatagag gagcatggtt agaatatgaa acagattcta atgatgtaat
     5761 atctgtaaga gttgatagaa caagaaaaca accagttaca gttttactta gagctttagg
     5821 aataggaaca gatgcagaga taattgacct tttaggagaa gatgagagat tatctgctac
     5881 attagaaaaa gataatacta agacagtaga agaaggtctt gtagagatat acaaaaaatt
     5941 aagaccaggt gaacctccta ctgtagaaag tgcatcatct ttattaaatg ctttattctt
     6001 tgacccaaaa agatatgacc tagcaaaagt tggtagatat aaattcaata agaaacttgc
     6061 tttatgttat agaataatga ataaaatttc agcagaagac ataataaatc cagaaactgg
     6121 agaagtattt gtaaaagctg gagaaaaaat aagctatgat ttggctaaag ccatacaaaa
     6181 tgcaggtata aatgtagtaa atctactaat ggatgatgat aaaaaagtta gagttatagg
     6241 aaataatttt gtggatatta agtctcacat tgattttgac atagatgatt taaacataaa
     6301 agaaaaagta cattatccaa ctttaaaaga aattttagat ggatatagtg atgaagaaga
     6361 aataaaagag gctataaaat caagaattaa agaacttata ccaaaacata tattattaga
     6421 tgatataata gcttcaataa gttatgagtt caatatattc tataatatag gaaatattga
     6481 tgatatagat cacctaggaa acagaagaat aagatctgta ggtgaattgt tacaaaatca
     6541 agttagaatt ggtctttcaa gaatggaaag agttataaaa gaaagaatga cggttcaaga
     6601 tatggaagct ataacgcctc aagcgttagt taacataaga ccagtttcag ctgcaataaa
     6661 agaatttttt ggtagttctc agttatctca gttcatggat caaacaaatc ctctatctga
     6721 gttaacgcat aagagaagat tatcagccct tgggcctgga ggtctttcaa gagaaagagc
     6781 tggattcgaa gtgcgtgacg ttcaccattc acactatggt agaatgtgtc caatagagac
     6841 tccagaggga ccaaatatcg gtcttataaa ctctctagga acttatgcaa aaataaatga
     6901 atttggattt atagaatcac catatagaaa atttgataaa gaaacatcaa cagttactga
     6961 tgaaatacat tatttaactg ctgacgaaga agatttattc gtaagagctc aggcaaatga
     7021 gccattaaca gaagatggta aattcgtaaa ccatagagtt gtttgtagaa ctgtaaatgg
     7081 tgctgttgaa atggttcctg aaagtagagt agattacatg gatatatctc ctaagcaggt
     7141 tgtatctgtt gccactgcta tgataccttt cttagaaaat gatgatgcca accgtgccct
     7201 tatgggagcg aacatgcagc gtcaggcagt gcctctagtt agaagagaag caccaattat
     7261 aggaactggt atagaataca gagcagcaaa agactctgga gcagttgttg ttgctagaaa
     7321 ttctggtata gcagaaagag taacagcaga tgaaataatt ataaaaagag aagatggaaa
     7381 cagagataga tataatctac ttaagtttaa acgttcaaac tcaggtactt gtataaatca
     7441 aacacctata ataaataagg gtgaccagat aataaaaggt gatgttatag cagatggtcc
     7501 agcaactgat ttaggagaag ttgcacttgg aagaaactgt cttatagcat tcatgacttg
     7561 ggaaggttat aactacgagg atgctatatt aataaatgaa agattagtta aagaagatag
     7621 attatcaaca attcatatag aagaatatga atgtgaagct agagatacaa aactagggcc
     7681 agaggaaata actagagata tacctaatgt tggagatagt gcaatcaaaa acttagatga
     7741 tagaggtata ataagaatag gtgcagaagt agactcaggt gatatattag ttggtaaggt
     7801 aactccaaaa ggagaaactg aacttactgc tgaggaaaga ttacttcgtg cgatattcgg
     7861 agaaaaagcc agggaagtta gagatacttc acttaaagta cctcatggtg aatctggtat
     7921 aatagttgat gtaaaagtat ttactagaga aaatggagat gatttatctc caggagtaaa
     7981 tgaattagta agatgttata tagctaaaaa gagaaagata aaagttggag ataagatggc
     8041 tggtcgtcat ggtaataaag gggttatctc gagagtatta cctgaagaag atatgccatt
     8101 catggaaaat ggaacgccat tagatataat acttaaccca caaggtatac catcacgtat
     8161 gaacatcggt caggttctag aagttcattt aggacttgct gctaagacat taggatggta
     8221 tgtagcaaca tcagtattcg atggtgctaa tgagtatgac ataatggatg ctcttgaaga
     8281 agctggatat cctagagatg gtaaattaac tttatatgat ggtagaacag gtcaatcttt
     8341 tgacaataga ataactgttg gatatatgta ttacttgaaa ctacatcact tagtagatga
     8401 aaaactacat gcaagaagta ctggaccata ctcattagtt actcaacagc cactaggtgg
     8461 taaagcacaa tttggtggac aaagatttgg agagatggag gtttgggctc tagaagcata
     8521 tggagcggct cacatacttc aagagatact tactgttaag tctgatgatg ttgtaggtcg
     8581 tgttagaact tatgaagcta tagttaaagg tgaaaacata cctgagccag gaatcccaga
     8641 atcatttaag gttcttataa aggaacttca aagtttatgc ttagatgtaa aagtattgac
     8701 agatgaagac caagaaatag aagtaagaga atctgtagat gaagatgata cgataggtga
     8761 atttgagcta gacgttgtaa atcacatggg agaagttgaa gaaagtaata taatagaaga
     8821 aattgaagat gattttgtgg aagccacaga agatgaggat atagagaatt tagaagaatt
     8881 cactgaagat gatttatttg aagaagaaat agattttgaa agtgatgact ttgatatgta
     8941 aatttaatta atgtaaatct caaatagaag ggagagaact ccttgtttga attaaacaat
     9001 ttcgagtcga taaaaatagc attggcttct ccagaaaaaa taagacaatg gtctagagga
     9061 gaagttaaaa agccagaaac tataaattac cgtactttaa agccagaaaa agatggtctt
     9121 ttctgtgaaa ggatatttgg accacaaaaa gactgggagt gtcattgtgg taaatataga
     9181 agagttagat ataaaggtgt agtttgtgat agatgtggag tagaagtaac taaatcaaaa
     9241 gtaagaagag agagaatggg acatatagag ctagctgctc ctatgtctca catctggtac
     9301 ttcaaaggta taccaagtag aatgggactt ttacttgata tgtcaccaag atcgttagaa
     9361 aaaatactat atttcgcctc atatgtagta gttgatccag gtgaaactgg attgaatgaa
     9421 aaacagttac ttacagaaaa agaatacaga actgctcttg aaaagtatgg atatactttt
     9481 actgtgggta tgggtgctga agctgtaaaa acattactac aaaatataga tttagaacaa
     9541 caaagtaaag acttaagagc ggaattaaaa gatagtacag gacaaaagaa agtaagaaca
     9601 attagaagat tagaagttgt agaagcattt aaaaaatctg gaaataaacc agaatggatg
     9661 attttagatg caataccagt aataccgcca gatttaagac caatggtaca acttgatggt
     9721 ggaagatttg caacttcaga cctaaatgat ttatacagaa gagttataaa tagaaataat
     9781 agacttaaaa gactattaga gcttggagct ccagatataa ttgtaagaaa tgaaaaaaga
     9841 atgcttcaag aagctgttga tgcattgata gataatggta gaagaggtag acctgtaaca
     9901 ggacctggaa atagaccact taaatcttta tcagatatgt taaaaggtaa gcaaggtcgt
     9961 ttccgtcaaa acttacttgg taagcgtgtt gactactcag gacgttctgt tatagttgtt
    10021 ggaccagaac ttaaatttta tcaatgtgga cttccaaaga agatggcatt agaattattc
    10081 aagccatttg ttatggataa attagttaaa gaaggttatg cacataatat aaaaagtgcg
    10141 aaatctatag tagaaaaagt taagccagaa gtttgggatg ttttagaaga tgttataaaa
    10201 agtcatccag ttcttcttaa ccgtgcaccg actctacata gattaggtat acaagcattt
    10261 gaaccaatct tagttgaagg taaggctata aaactacatc ctcttgtatg tacagcttat
    10321 aatgcagact ttgatggtga ccaaatggca gtccatgtac ctttatcagt agaagcacaa
    10381 gcagaagcaa gattcttaat gctttctgta aataatatac ttgctcctaa agatggttca
    10441 cctataacta ctccatctca ggatatggtt ttaggttgct attatctaac aatagaagcc
    10501 caagatggag ctaaaggaac aggtatggta tttaaagact ttaatgaact attacttgct
    10561 tattacaata aatcagttca tctacatgca ttagtgaaac tgaaggtaac acttgaagat
    10621 ggaagaagtt cattggttga aagtactgtt ggtagattta tatttaatga aaatatacct
    10681 caagacttag gttttgtaga tagaaaagaa aatccatttg cacttgaagt tgatttctta
    10741 gcagataaaa aatctcttgg taaaataata gataaatgct ttagaaaaca cggaaataca
    10801 gaaacagctg aattactaga ttatataaaa gctctaggat ttaaatattc tacattaggt
    10861 ggtataacag ttgctgttga tgatatgagt gtcccagaag aaaagaaagt atttatagct
    10921 gaggcagaag ctaaagttga taagtatgaa aaagcataca gaagaggtct aatctctgat
    10981 gaagaaagat atgaaaaagt tatagagaca tggacagaaa caactgataa agttactgat
    11041 gctcttatgg gtggactaga tagattaaat aatatatata taatggcaca ttcaggagcc
    11101 agaggttcta aaaaccaaat tagacagctg gcaggtatgc gtggtcttat ggcaaatgca
    11161 tctggtaaaa cagtcgagat accagttaaa tctaatttcc gtgaaggttt atcagtacta
    11221 gaatacttta catcttcaca tggtgccaga aaaggtcttg ccgatacagc tatacgtaca
    11281 gctgaatctg gatatttaac aagaagactt gttgatgtaa gtcaagatgt tattgtaagg
    11341 gaaatagatt gtggaacaga agatactaca gagatttatg ctataaaaga aggaaatgaa
    11401 gttatagaag agatatatga tagaattgta ggaagatata ctatagaccc tatattaaat
    11461 cctgaaactg gagaagttct agttgaggct gattcaatga tacaagaaga tgaagcagaa
    11521 actatagtag ctttagggat tgaaaaaatt aggataagaa cagttcttaa ctgtaaaact
    11581 aatcatggag tttgttctaa gtgttatggt agaaacttgg caacaggaaa agaagttaat
    11641 ataggtgaag cagttggtat aatagcagct caatctatcg gtgagccggg tactcagctt
    11701 acaatgcgta cattccatac tggaggagtt gcaggagctg atataactca aggtctacca
    11761 agggttgaag aattatttga agctagaaaa ccaaaaggat tagctgtaat aactgaagta
    11821 tctggtagag ttgaaattga tgaaacagga aagagaaaag aagtaaatgt aataccagaa
    11881 gaaggcgaga ctcaaacata tgtaattcca tatggttcta gattaaaagt taagcaaggt
    11941 caaatgctag aggctggaga ccctctaaca caaggattta taaatcctca tgatatagta
    12001 agagtaaatg gtgttaaggg agttcaagaa tatatagtta aagaagttca aagagtgtat
    12061 agacttcaag gggttgatgt taatgataag catatagaag ttatagtaag acaaatgcta
    12121 tctaaagtaa aagttgaaga cccaggagat acagatttat tgccaggtgg atatgaagat
    12181 gtattaacat tcaatgaatg taataaagat gctatagata aaggtctaag accagcagtt
    12241 gctaaaagag ttttacttgg tataactaaa gcatctcttg caactgattc attcttatca
    12301 gcagcttctt tccaagaaac aacaagagta ttaacagagg cagcaataaa aggtaaagaa
    12361 gaccacttaa taggacttaa agaaaatgtt atattaggta agttaatacc agcaggaaca
    12421 ggaatgaaga aatatagaaa tatagctgtt gaaaaaattg aagattaata gcatatagag
    12481 taaagaaatc ttgacaacca ttatttgtgg tgttaaaata gtaaagtgtg ttgtttaaaa
    12541 gtacactaag ggcttgaaca agttcttagt gacttttaga tggcaataaa tgagaactaa
    12601 aaagcatcaa gctagaaaag tgctaatatc acatatttat gtgttataga cattaaattg
    12661 taagtaacga atgttacaat cagtcagtat gaatattcat attgatgtag ttggcggtca
    12721 ctgataagat cgaaaatttg gaaggaggtg cagatgatgc caacaattaa ccaattagtt
    12781 cgtaaaagta gaaaagcgct agagaagaaa tctactgctc cagcattaca aaaaggttac
    12841 aactctttaa ataagaaagt aactgacgct agtgccccac aaaaaagagg ggtttgtact
    12901 tcagtaaaaa cagttactcc taagaagcct aactcagctt taagaaaagt tgccagagtt
    12961 agattaacta atggtataga agtttctgct tatataccag gagaaggaca caacttacaa
    13021 gagcatagtg ttgttcttat aagaggagga agagtaaaag accttccagg ggttagatac
    13081 catatactaa gaggtactct agatacagca ggtgttgata agagaagaca atcaagatct
    13141 aaatacggtg cgaagagacc taaagaagct aaaaaataaa acaattaatg attgattata
    13201 gttcggtgct ttaaatatat atatatttac ggcatcacgg cattaaatga tttatgtcga
    13261 gtacctatga tttaagttaa ataattaagg agggaagcga aatgccaaga aaaggtaata
    13321 ttccaaaaag agaagtttta ccagatccta tgtatgggag taaggtagtt actaaattaa
    13381 taaataatct aatggtagac ggaaaaaaag gaaagtctca aagaatagtt tatgatgctt
    13441 tcgcaattgt tgctgaaaag acaggagaag aagctttaga agtatttaat aaagcaatgg
    13501 ataatataat gcctgtttta gaagtaaaag caagaagagt tggtggggca aattaccaag
    13561 tgccaatcga agttagacct gaaagaagac aaacattagg tttaagatgg ctagtaaaat
    13621 atactagagc tcgtggagaa aaaggaatgg ttgaaaagct agctaaagag ataatggacg
    13681 cagctaacaa cactggagct tcagttaaga aaaaagaaga tactcataaa atggcagaag
    13741 ctaataaagc atttgctcat tacagatggt aggatatata gatatcttat aaattcaagg
    13801 atatcataag gaggataacc atggctagaa agtttccttt ggaaaaaact aggaatatag
    13861 gaatcatggc tcatatagat gcaggaaaga ctactactac agaaagaatc ctgttttata
    13921 ctgggcaaac tcataaaata ggagaaactc atgaaggagc ttctcaaatg gactggatgg
    13981 agcaagaaaa ggaaagaggt ataacaataa cttctgccgc tactactgct tcatggaaag
    14041 accatagaat aaacataata gatactcctg gacacgtgga cttcacagtt gaggttgaaa
    14101 gatctctaag agttcttgat ggttcagttg ctgtattctg tgcaaaaggt ggggttgagc
    14161 ctcaatctga aaatgtatgg agacaagctg aaacttatgg agtacctaga atagctttta
    14221 taaacaaaat ggatatatta ggtgcagatt tctacaatgt tataagcatg atgaaatcta
    14281 gattaaattc taatgcagtt cctatgcaat taccaatagg taaagaagat tcatttatag
    14341 gtataataga cttattaaaa atggatgctg ttatatacaa agacgaccta ggggttgaaa
    14401 tggaagaaac agatatacca gaagatatga aagaattagc tgctgagtgg agagaaaaat
    14461 tagttgaatc agtagctgaa actgatgaag aattaatgat gaaatacctt gaaggtgaag
    14521 aacttactat agatgaatta aaagttgcta taagaaaagc tactatagct tgtgaaatga
    14581 acccagtttt ctgtggtaca gcttatagaa acaaaggtgt tcagttagta atagatgcag
    14641 ttttagatta cttaccagca ccaactgata taccagctat aaaaggtata ttagcagatg
    14701 gagaagaagc tgaaagacat tcttctgatg aagagccatt ctctgcatta gcattcaaaa
    14761 taatgactga cccattcgtt ggaaaactag cgttcttcag agtttattct ggaacattag
    14821 aatcaggttc ttatgtttta aatgcaacta agaataagag agaaagaatt ggtcgtatat
    14881 tacaaatgca tgctaataca agagaagaaa taactaaagt gtatgctgga gatatagcag
    14941 cagcagtagg attaaaagat actactactg gagacacatt atgtgaccct gctaatccaa
    15001 taatacttga atctatggaa ttccctgagc cagttatatc tgttgctata gaaccaagtt
    15061 ctaaagcagc tcaagaaaaa atgggtatag ctcttcaaaa attagctgaa gaagacccaa
    15121 ctttcacagt taagactgac caagaaacag gacaaactat aatatctggt atgggtgaat
    15181 tacacttaga gataatagta gatagattat taagagaatt taaagtagaa gctaaagttg
    15241 gtgcaccaca agttgcatat agagagacta taactcaacc agttgatgtt gaatacaagt
    15301 actctaagca atcaggtggt agaggacaat acggtcacgt taagattaga gttgctcctc
    15361 aagagccagg tgaaggatac aagttcacaa acaaaactgt tggtggatca gtaccaaaag
    15421 aatatgttgg tccagttgac atgggtatac aaggagctat gcaatctggt atagttgctg
    15481 gatatccagt tgtagacgtt gctgttgagt tatatgatgg ttcttaccat gaggttgact
    15541 cttctgagat ggcgtttaag atggctggtt caatggcatt caaagatgct atgaagaaag
    15601 gtaatgcagt tcttttagag ccttacttca aagtagaagt tgttactcca gaagattaca
    15661 tgggagatgt tatgggtgac ttaaactcaa gaagaggatt aatccaaggt atggaagcta
    15721 gaagtggtgc acaagttata aatgcattcg ttccactttc agaaatgttt ggatactcaa
    15781 cagacctaag atcttcaact caaggtcgtg caacttacac aatgatattc gatcactatg
    15841 agcaagttcc agcttcagtt gctaagaaaa tagctgaagg aaaataattt aaaaataatt
    15901 aataatataa atataaaaaa taatcagcta gatgagatat atctcatcta gcacaagat
//
LOCUS       DAEPOZ010000073        15855 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.073, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000073 DAEPOZ010000000
VERSION     DAEPOZ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15855
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.073"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..616)
                     /locus_tag="KR440_001728"
     CDS             complement(<1..616)
                     /locus_tag="KR440_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0698781.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNVSTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEEDMD"
     gene            complement(948..1730)
                     /locus_tag="KR440_001729"
     CDS             complement(948..1730)
                     /locus_tag="KR440_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBH0698782.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKMVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKENVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            complement(1733..3952)
                     /locus_tag="KR440_001730"
     CDS             complement(1733..3952)
                     /locus_tag="KR440_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBH0698783.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            complement(4216..5313)
                     /locus_tag="KR440_001731"
     CDS             complement(4216..5313)
                     /locus_tag="KR440_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698784.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSNIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            complement(5629..6822)
                     /locus_tag="KR440_001732"
     CDS             complement(5629..6822)
                     /locus_tag="KR440_001732"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBH0698785.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     regulatory      complement(6959..7062)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            7251..7760
                     /gene="yyaC"
                     /locus_tag="KR440_001733"
     CDS             7251..7760
                     /gene="yyaC"
                     /locus_tag="KR440_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HBH0698786.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     gene            complement(7871..8299)
                     /gene="fabZ"
                     /locus_tag="KR440_001734"
     CDS             complement(7871..8299)
                     /gene="fabZ"
                     /locus_tag="KR440_001734"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBH0698787.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEIEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(8315..9334)
                     /locus_tag="KR440_001735"
     CDS             complement(8315..9334)
                     /locus_tag="KR440_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBH0698788.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            complement(9531..9791)
                     /gene="spoIIID"
                     /locus_tag="KR440_001736"
     CDS             complement(9531..9791)
                     /gene="spoIIID"
                     /locus_tag="KR440_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HBH0698789.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            complement(9889..10557)
                     /locus_tag="KR440_001737"
     CDS             complement(9889..10557)
                     /locus_tag="KR440_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HBH0698790.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFNDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            complement(10623..11687)
                     /gene="spoIID"
                     /locus_tag="KR440_001738"
     CDS             complement(10623..11687)
                     /gene="spoIID"
                     /locus_tag="KR440_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HBH0698791.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIEDKGNKEEKSTVSYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            complement(11774..13027)
                     /gene="murA"
                     /locus_tag="KR440_001739"
     CDS             complement(11774..13027)
                     /gene="murA"
                     /locus_tag="KR440_001739"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBH0698792.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            complement(13048..13785)
                     /locus_tag="KR440_001740"
     CDS             complement(13048..13785)
                     /locus_tag="KR440_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HBH0698793.1"
                     /translation="MKKVKLAISFFILMIIGMTISYADTSINTRYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGKTLIYIATPIIKLDY"
     gene            complement(14269..15564)
                     /locus_tag="KR440_001741"
     CDS             complement(14269..15564)
                     /locus_tag="KR440_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HBH0698794.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKMQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKRAVDFSANWLNVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWGEESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSITPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLVVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSNSVSLEEYRNIDNTMRNKSEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
BASE COUNT         4830 a         2958 c         1636 g         6431 t
ORIGIN      
        1 tatccatatc ctcctctata gtaagcatta tgttttttac aattattata tctactatat
       61 ggaagtactc tatttcatct atgtttaatc tgtatttatc tatgtattta aataagaatg
      121 atactaacat tattcttatg tctttctcat ccccttcaat atatatacca ctgccttttt
      181 tagtcgagac atttaaatta taattattta atgctactga taatttactt atttcttctc
      241 tgacaatatt tttatttatg tacaacttat tagataaatc atttaaagta acttttttat
      301 tattacaaag tagtaaattt aatatctcaa cttgcttatg aagctcacta tttactcttt
      361 taacttcacc tcgcaacttc tgatttacca gtattttgtc tgttccatct ccactaaatt
      421 taattccaat attaggtttt cttttgatat aagcattaca aaaagtagat aaccattcat
      481 caatatattt gcaatcattc cttattgtcc tttctgaaca ctcaaaatca ttggcaatct
      541 tattaacatt tataaactct tcttttaata gcaatgttgt taataaattt tcttgtcttt
      601 tattcaaaca tatcacctct ttatgactca acctaaattt attatatata aaaaagagct
      661 atacataaag gtatgctagc tctcttttaa tatgatattt agctgacctg gtttttgacc
      721 ccacactcgc ctactggagg gttcgcttgg gttcgccgca ctactactct ctctcgattt
      781 tattatcggt aggtcttact tctaagccgc accatgatca ttaacccaat ttttagtgtt
      841 aacttacgtg gatccctttg cctgcgactg gcttggactt tcaaccacag acctaaatat
      901 cacttattca ttttaatatt ttaaactaaa attgtcaata tataagatta ttccataata
      961 taattatcat ttgcttttgt aagtagagtc aatacaaaaa ccttattagc acctgataac
     1021 tttaaaagtt tagagatttc gtttgttgtt agaccagtag taaatatgtc atctattaaa
     1081 agtatatttt tattatttat taatttaaca ttttctttaa caacaaatac attttttaat
     1141 tcttcttttc gttctttctt attcaattta tatagcattc ttgtatattt ttttctactt
     1201 atgcaatcta ataatggaat atttactatt ttacttaaat taaatgctat tttttgtgcc
     1261 tgattaaaac ctcttttatt tagccttttt ttatgaagtg gaacaaataa tatataatca
     1321 aattttatgt tttctaaata taatttttct ttcattattt gagctatata cttagccata
     1381 aaggtttttt ggttatactt cagccctaac accatctttt ttgtcacatc attatattca
     1441 atacaagata ttgatttatc aaaataaaag tctttattaa aacaataact acactcttca
     1501 ataaattctt tttcaattga atgacgtatt attggttttc cacacttcat gcaaccatct
     1561 tgtataaaat tcatttcttt aaaacaactt ttacatattg aataagtatt tgttttcttt
     1621 atggatttat cacaaattat gcaacttata ttctcaggat atataaaatc caaacattta
     1681 ttaatcatat ttttaaaatt cttaataaaa ttatcattgc atattttcat atttattcta
     1741 tcaacaaccc ttcttgttta aatttattta gcttatatcc taaatttgaa tacctttgat
     1801 taactctatt attttttatc atatattcaa gatatttcac atctccaact aatacaacta
     1861 atttttttgc tcttgttact gctgtatata ataaattacg gctaagtaac ataggaggag
     1921 cccacgctat tggcaatact acaactggaa attcacttcc ctggctttta tgtattgttg
     1981 tacaaaaact atggtcaatt tcatctaatt catcatatag gtatgataca attttagtct
     2041 ggtcaaataa tatataaata gttttcttat ctttatctat gtgataaaca tatcctatat
     2101 ctccattata gataccttct ccactttctt tttggtcttc tgtttcccat ttttttgtat
     2161 aattattttt tacttgcatt accttatcgc caactctaaa taatctttta gaaaaacttt
     2221 cttcaacttt aaatttttct tttttgttaa gatacttctg taactctata ttgagattag
     2281 taactcccaa ttcacctttt ctcattgaag ataaaacttg tatatctttt aatttatcaa
     2341 ctttataaaa tttaggtaat ctttcattaa ccaatcctat tatttcattt aaaatttctt
     2401 cattagttga ttttcttata aaaaagaaat ctttaccttt tgtatttaga taaagtggtt
     2461 ctccattgtt tatcttatgg gcatttacaa ttatcatact ttcttgcgct tgtctaaata
     2521 tttcatttaa tttaactaca tttataatat tagaattaat catatcctta agtacatttc
     2581 cagcacctac agaaggtaat tggtcactat ctccaactaa aattactctt gttcctaact
     2641 ttatagctct aagtaaattg tacattaaga taatgtctac cattgatacc tcatcaacaa
     2701 ttattacatc tgaatttatt gggtcttctt catccttaaa aaaagttaaa tcatcatctg
     2761 ttgaaaatcc catttctagc aacctatgta tagtttttgc ttctttatta gaagtttcac
     2821 tcattctctt tgctgctctt cccgttggag ctgccaaagt cacactttta ccattatttt
     2881 caaatatatc tatgatagca ttaatggtag ttgttttacc agttcctggt ccaccagtta
     2941 taattaatac tccactatta acagattctt tgactgctaa aatttgtttt tctgctaact
     3001 taattttctt atcttcttct aaaacattaa tctcgttatc tatatctatt tttaagtctt
     3061 caaattcata ctgtgataat tttactattt gactacatac tccattttca gctaaatagt
     3121 atggcataag gtatataaga ttttcattgt ttactttctc tatatgtatt ttttgattat
     3181 aaactagcat cattacacac tcttttatta tttctccatt tagctccaat aatttttccg
     3241 aatcttgtat tagaacatgc tctggtaaat atgtatgccc attacttaaa gatttattta
     3301 aagtgtataa gattccctga caaattctat ctttagagtt tttatctata ccaattttac
     3361 ttgctatact atcagctact ttaaatccaa ttcctcttat atcttcagca agttggtatg
     3421 ggtttttatt tataacttct attgcactgc ttttatattt tttatatatt tttaggcagt
     3481 aatttggtgt tattccaaaa ggagatagct gtattattat atttctcaat tctctgtctt
     3541 cttcataact ttttactatt tgttttactt ttttactccc tataccttct acttcttgta
     3601 atttttctgg tgaattttgt atcacctcta acgtatctac accaaactta tcaattattc
     3661 tttttgccat tttttcacca ataccatgta tcattccaga agataagtaa acataaattc
     3721 cttctaagga tgatggcgtt actggcataa aactattcac ttcaaattga gtcccataaa
     3781 tcttatgatt tacccacttt ccttcaactt ctatgctttc tccaattgcc aaagtaggca
     3841 tacatcctac aaccacaatt tcatcatttt catttaccag gtgagctatt gtataaccat
     3901 tatcttcgtt tttaaagact atttcactta tcattccttc taatttttcc atttttatct
     3961 cctaaaaata ctaataatcg ctccatttaa atgttagtat aattttatat attaaaacta
     4021 tgataatcgt agaaaagttt tatattttta tacttaacgt aatttccaac tattatacat
     4081 tttatcagaa acaaaaaaaa tctgcatcca taaaatatgg taccatgaaa tacaggtacc
     4141 atatttaaat agctatttta ttttaattac tctatattta gagtataaat taaattccat
     4201 aagtatatta cagtactaac ttatttcaga tttattctcc ttgtacccct cagttttagt
     4261 aattcttcta taaagcatac atagtaaaat taaaattccc atgtaacctg tatatggata
     4321 tagtgtacct actaacttat caaatggcag taaacctcct atacaagcta atatacttac
     4381 cactattgtt ataattttaa atttagggtg gtcatcttct acaaatcggt tagttactga
     4441 ccataacaat ggtacagctg tagtgtatat tcctagtaaa agtactactg agaataaaat
     4501 acctattatt ggagatatct tatcagctaa gtaaagtgct ggtatctctt ttgtgtatat
     4561 atttcctatg tttgaaagta atgccaaatt catcattata gctgctgcca taagtgctac
     4621 tccacctact ataccacccc atacagcatc tttcttattt actgcagaag ctcccattcc
     4681 tgtcaagaat gttattacta ttattacatt gtaacaagta taaagtactc ccgagaaata
     4741 tccatttgat gtagcagttt taatatttaa actatcaacc atatttccag catttgataa
     4801 agcatcaata ttgctaaata aactaattgc tcctactaaa agtgtaaata ttattattat
     4861 aggtcctata ttacctagta ttttagaaag ccttgtcaaa cctaatgaaa cagttattag
     4921 ggaaacgatt gccataccaa tcctacctac atatggattt aatccatagt actctgatag
     4981 tgtagctcct gctcctgcaa tcattattac taatgttcca aataaaaaca atggaccaaa
     5041 ccattcaaaa aatgttccaa gatattttcc acaatatact tgataaattt ttattggctc
     5101 cttaagtttc aattctcttc ccttatctat aacttctgct ccaaaccatg aaaatagtac
     5161 catagagatt attccaccta taatacccgc atatccataa aacgtaaaaa attgcagtat
     5221 ttcttgacca gttgcaaatc cagaacctat tacagtagca acataagctc cagcaaaact
     5281 tataaccgtt ttaatattta ctttgcctga catacactac tcccccccaa ttaaaattat
     5341 ttttaaagtg taagtttatt aagattatat tagactcatt attgtgatat ttcaatatat
     5401 ttttaattat ttaacattag ttaacacatt cttaaccatc ctttaacata tatcccataa
     5461 tatgtataaa tttagtagag tattatataa aatgtattga ccagattaaa tactgtttat
     5521 tcattatatg atgataaatt aacatttatt aaaaatattt aactcataat aaaaaaactg
     5581 ttagaaagta atcgtaaaat tacattctaa cagcttttta gttaaaaatt attcacctaa
     5641 agcatctttt cttaactgct caaccttgtc agttctttcc caaggtaaat ctatatcagt
     5701 tctaccaaag tgaccataag cagcaacctt tttataaaga ggctttctta aatctaagtc
     5761 tcttattatc gctcccggtc ttaaatcaaa gtgtttattt actaattcaa caagtttttc
     5821 ttctgaaacc ttgcctgtcc caaaagtatc tataaatata gataatggtc tagctatacc
     5881 tatagcatat gcaagttcta tctcacattt gtctgcaaga ccagccgcaa caatattttt
     5941 tgcaacatat ctagctgcat aagcagcaga tctatcaact tttgtagggt cttttcctga
     6001 gaaagctcct ccaccatgtc tagaatatcc accataagta tctattatta tttttcttcc
     6061 tgtaagacct gtatctcctt gtggtcctcc tataacgaat cttcctgttg gatttatata
     6121 aattttagtc tcttcatcaa gtaattctgc tggtataact tccttaataa catgattaat
     6181 taaatcttct cttattttat catttgatac tgtttcacaa tgttgagctg atataagaac
     6241 tgtatgtact cttacagctt tacttccttc atattcaaca gtaacttgag tttttccatc
     6301 tggtcttaaa taatcaacta aacctgtttt tctaacttct gtaagccttc ttgataattt
     6361 atgtgccaaa gatatcggaa gtggcattaa ttctggagtc tcattacatg caaatccaaa
     6421 cattatacct tggtctccag ctccaacttt ttctatttct tcttctatct cttctccagt
     6481 cttactttct agaccttcat caactcccat agctatatct ccagattgtt catcaataga
     6541 agttataact gcgcaagtat cacaatcaaa tccatatttt gctcttgtat atccaatttc
     6601 tcttactgtt tctcttacta atttaggaat gtctacataa gcagatgtac ttatttctcc
     6661 tgctactaaa actaaaccag tagtaactgt agtttcacaa gctactctag attgtgggtc
     6721 tttttctaat aatgcatcta atattgaatc tgatatttgg tcacatatct tatcaggatg
     6781 tccttctgta actgattctg acgtaaataa atgtcttgcc atttttattc ccccttattt
     6841 attcttaatt tttattatag gttttacctt atttttaatt ttagaaattt actcaaaata
     6901 aaaaaacctc tcctttataa gaagaggtta ataatcgtaa ttacatacta attaactgac
     6961 ctcatctttc aagacttttg tcttgtagga attagcacct tttcccattt gggtggttgc
     7021 tgggcttcac agggcctatc cctcagcctc tcttgataag gtaatcaaga tttaagtttt
     7081 gttttacttt actagcttac tattttttta aaattttgtc aatattttta gtcatatttt
     7141 ttctattcct ttttttgtaa gacaaataaa attttctatc tatctgaaat ttaaagttag
     7201 gtctttttaa tgctttttat tcatatattt ttttgaaggg ggtgttccta ttgtatttag
     7261 ctaaagtaaa ctacagcgat gatgatgtta tacagatgct aagttcagtt ctaaaaacta
     7321 taattaatga aaatacgatt gttgtttgca taggaacaga cagggctata ggtgatactt
     7381 taggcccttt agttggaaca atacttaaga acagtaactt taaatacccc gtttacggta
     7441 cactagataa ccctattcac gccttaaata tatatgagtc tttagatact ataaaaaata
     7501 cacatataca aggtaacttc ttggctatag atgcttgctt ggggtcgcag agtaacatag
     7561 gtaatataca gattagagaa ggacctatcc tgcccggaaa aggtgttggt aaaaaactac
     7621 ctcaaatagg aaattattct atagtaggaa ttgtagataa aattgatgaa aataataaac
     7681 tttcatttaa taatatacgc cttagcttta tattagattt agctgaaaca atagccttgg
     7741 ctttattagt atccacctaa tctaaatata aactaaatga taaaattctt tatataaaaa
     7801 ggtagatatt ttagagtatc tgattaaata ctccaaatta tctacctttt tacttttata
     7861 tttcaagttt ttatttttct actaaagcaa acattaattc acctttacag acaagttctt
     7921 cacctacata tgccttagca tctgccttag ctatattttt tctaagagtt gtcatttcaa
     7981 tttccatagt taatgtatct cctggtctta cttctctctt gattctaact ttatctatac
     8041 ctgcaaatac acctagtttt cctttgtttt cctctacaga catcattgcc actccacata
     8101 cttgtgctag agcttctact attagtactc ctggcatcaa aggatattct ggaaagtgac
     8161 cttggaaaaa tggttcatta actgttacat tctttatacc aacagctctt ttcccaactt
     8221 ctagctctgt aatcttgtct actaataaaa atggatatct atgtggtatt aattttttta
     8281 tttcatctat atttaacatt ctaaactctc ctaattattt tcttttgaaa gacccaccag
     8341 tttcaagtaa gtctattgaa cctaaagcta ttcctgtacc ttttgcaaca caagataatg
     8401 gttcatctgc tattgtaaca gttattcctg tcctctgttc tattatttta tctaatccat
     8461 ataataatga acctcctcca gtcattatta taccgacatc tcctatatct gctgctaact
     8521 caggaggcgt tttttccaaa acagcatgag tagcaactac tatttgctca acacattctc
     8581 taagtgcttc caacatttct gttgaattta tagttataga actaggaagt cctgtaatta
     8641 aatttcttcc tgttatcttc atatattttt cttcttctct cttaaatgca gaacctattt
     8701 caaacttgat tttctctgct gttctttctc ctataagaag attgtgttgt tttttcatat
     8761 atttaactat agcatcatca aatctatctc caccaacctt tatagattca cttacaacag
     8821 ctcctcctag agatattaca gctatatcta cagtacctcc accaatatct ataatcatat
     8881 taccattagg ctgtgatatg tctacaccag ctcctatggc agctgctatt ggttcttcta
     8941 tgatatatac ttctctagct ccagcttgtc tcgtagcatc ttctactgct cttttttcca
     9001 cttctgtgac acctgttggt acacatacca taattcttgg cataaaaaat cttccaaatc
     9061 cctttttatc aactatctta tctataaaag attttaacat cttctcagtt acatcataat
     9121 ctgaaattac accatctctt aaaggtctta tagcgactat attacctggt gttctcccta
     9181 tcatcttttt agcttcttct cccacagcta aaacagaatc tgttcttttg tctatcgcca
     9241 caacagatgg ttcttctaaa acgattccct taccactaac ataaacaagc acatttgcag
     9301 tacctaaatc tatgcctata tcagctccag ccattttttc aactccttta tttataatat
     9361 tagattatat tataagccta ttttaatact cctataataa agcatataca aaaatctcgc
     9421 tttaatcaag agaaaataaa tcttttgaga gtattattat tttgctattt ataattactt
     9481 aatatatttc tttttataat ttaattcaat aaaaaaagta ggtttttaac ctacattttt
     9541 ttatgttcct tctcatattt taatttcgtt gctaatcccc ctctaatatg tctctctgat
     9601 ttatttttgt ccagcacatt tttaacttct ttggcaaggg atggatttat ttccttcaat
     9661 ctttctgtaa catccttatg aattgtactt ttacttactc caaatgtctt ggcggtttgt
     9721 cttactgtag tgtttttttc aagtatatat tttgctacca ctatggccct ttcctctata
     9781 tgagatctca aagattaccc ccctcacata atagctttaa tgatatatat gtaaaaacac
     9841 caaaaaatat aacaagctta accaaatatg attaaacttg ttatactttt acttaattag
     9901 actcattggg tctatagatt tttctccact atacgcctct aaatgaacat gtataccttc
     9961 ttcactttct atttgagaag tactacctac agtacctaga gattgtcctt ctgttacctt
    10021 ctgttctttc ttaacactaa catttttatc tagatttgaa tatactacta caatgttatt
    10081 gtcacttttt attttgactg attgcccata ttcttcatca ttaaatacat ctactactat
    10141 tccgtttaat agagatttta cttctttgcc tttagtacaa ctaatatcta cacctttatg
    10201 agtttcccaa acatcaagag ttttagaata actaggttct ttttctgaat atcctcttat
    10261 tactttatct ccaatgtaat ttaattttgt tgtactcgat ttattttcct cttttgactt
    10321 agctttttct aagttttgtt ttgaatccgt tgcagtaggg atagcatctt tcttgtcttt
    10381 ttcaattaaa tgtatttcct cttcactatc tttattagca ttttccataa ttcctttatt
    10441 agaagccaat ttatctacat tattatttgt aaaccaaact ccacctactg ctaataaaca
    10501 aacacataca aacaaagata aataaaaacc atctttttct aacagctttt tcttcatttt
    10561 gcacctccaa atatatttct acatttggat tattgacgtg tttttggcat tttatacaat
    10621 tattagtata tatcttttat ttttgtatct gtgtagtaat gtgataatat atcataatat
    10681 ttatagccct cttctgccat tccttctgct ccccactggc tcataccaac accatgacca
    10741 tatcctttta ccacgaaatc tatgtatcct tctccaaatt ttatgtcaaa gtttgcagag
    10801 ttcaatttaa aaacagttct aatgtctttt ccagtcaatt ctttatttcc taattttatt
    10861 ttttctacag ttccggcatc acttctttta gttattgaaa cttgtttact taaattattc
    10921 acatctatca caatagtgct atatgccctt ctaagacttt taacaaaatc tgtgttactt
    10981 attttaagtg ttgacgcaaa tttaggggaa tacttatcat aaggactttc cacagacttt
    11041 aaataaggat attttgcaga aaatacttct tcactatttt ctgtttttcc tgaagatgta
    11101 gaaaaataaa gaggaagtat cgccttatca ttataagtta ttatttgacc tttagttcct
    11161 ctaactgctt cttggatttt ggaatattta ttcttaatcc attcttcacc atttaatttt
    11221 ttaagagtat cataactctt atattcttga caatgtttat aatcagtaca tactactgca
    11281 tttttatgtt tactagattt accatgttcc tgtttatata ctacatatgt tctagctgct
    11341 acagattgag cttttaaagc ttctatatca aactctgaag acatctcacc agctagtact
    11401 ccacataaat agttctccat gtccatcttt tcggttttcc ctgttatatg attatataca
    11461 ttgataattg gtgctttttt atttacagtt tcatagctca ctgtactttt ttcttcttta
    11521 ttacctttgt cttcaatatc acttttttta atagttttgt ttatagatac tgggctctct
    11581 ggtttttcag ttaattctac atttttataa gaaactaaag ttataagtga tggaactaac
    11641 acagagcaag tcacaaatcc taacaaaaca accaatgggt tcttcatata ctctcctcct
    11701 tagcttttaa aaccttgtat ttaagtatat gaacaaccct attaatttat aactttcttc
    11761 tataaaccaa ttattaatct tctactattt cgatttgacc acctagtgct gttatcttct
    11821 tatctatatc aacataacct ctttggatat ggtatatttc tcctatttga gtttctcctt
    11881 ctgctataag accgcataaa ataagggctg cccccgctct taaatcagta gcgtttactg
    11941 ctgcaccgtg tagctcatct acaccattaa caaccgcact tcttccatct atttttatat
    12001 tagcacccat tctattaaat tcagcaacat gcataaatct attttcaaaa acagtttcta
    12061 taactacacc agttccattg gctacagtaa gcatagccat aaattgtgct tgaacatctg
    12121 taggaaatcc tggatgtggt aaagttttta tatctattgg ttttaatact tttggaccta
    12181 caactcttac agaattgtcc atttctgtta tttcgcaacc agcttctctt aatttagcta
    12241 caactggttt taaatgctcc attaaaacat tttcaacagt tatatcacct tttgtcatag
    12301 ctgctgcaac catataagtt gctgcttcta ttctgtctgg aattacatta tgttctgctc
    12361 cttttaactc tttaacacct tttattttta tagtattagt acctgcacct ttaacatctg
    12421 ctcccatttc gttcaagaag tttgctaaat ctactatttc tggctcttct gctgcattct
    12481 ctattattgt agtcccttct gctaaagaag ctgccatcat tatgttttca gttgctccaa
    12541 cagatgggaa atctaaatat aatttatttc ctactaactt ttctgttgct gcttcaacaa
    12601 aaccatggtc catttctatc tttgcaccta aggctttaaa tccttttaaa tgtaaatcta
    12661 taggtcttgt tcctatagca catcctcctg gcatagaaat tttcgtacta ttaaatctag
    12721 caagcaatgg tcccataacc aaaaaagaag ctctcatctt tcttacaagc tcatatggag
    12781 cttcacaagt ttttatattg cttgcatcaa cagtaagtgt attttcttta tattcaactt
    12841 ctgctcctac atgtcttaat aaatctgata taacatgaac atctcttaaa tttggtactc
    12901 catttaatgt tgattttccg tttgctaata aagtcgctgc tattattggt aaaactgcat
    12961 tttttgcacc atctatttta acactacctt taagtggatt actctttttt actattatct
    13021 tagccatatt ttttgatctc ctcataatta ataatctaat tttataattg gtgtggcaat
    13081 atatatgagt gtttttccgt catcctcatt atatttaatt cctatattta aattaatttt
    13141 ttcccctaaa tattctaatt cataatcatt taatacttta ctatatgctg ttatagataa
    13201 aaagtttacg tctttaactt catctatttg cttagcactc atattatata atattttttg
    13261 taaaatatca tcatatttgt tgttttgtaa cctttttgtg tattctccaa caatacaagc
    13321 agacacatct acttgagatg aatatttatt taacacattt tctacgttac tataaataac
    13381 ctctttatgt ttatatactt tgttatccaa tatgtcaact actatgtaac tttctttgtt
    13441 atttttattt attcctttta tagaaatttc tttatcgcta ccttttatag tagcacataa
    13501 ttgtatttta tcgccagacc ctttttgatt ccaatttatt ccttttttat ccaactttag
    13561 attgttaatt atttctataa aaatattatg aaattcttta tttgataaat tatgatttat
    13621 aacagaatta attttaacat tataaaattt aaaatttgct tctgatattt caagagcttt
    13681 acttaatcta ttgtatctag tatttatact tgtatctgca taagatattg tcattcctat
    13741 aatcattaat ataaaaaagc ttatagctaa ttttactttt ttcatagtaa aaaatccccc
    13801 ttaaatatgt ttttctgtat aatagaatta ttaacaaatt taagagagat atactttctc
    13861 aatatgtatt tttatacatt tttgtatttt tcatctaata atctctttca aactcttcaa
    13921 tctcaatttt ctctatcctt ctcctggact tgccaattcc ttatatgtat caaatacatc
    13981 ttgcattaat tcttatatat tatcttctgt aatataaatt acttagtcta tacttaaatc
    14041 tataatctat aatctataat ctataatcta taatctataa tctataatct ataatctata
    14101 atctattcat attttaccag caaaataaac agataatact tttttatcaa tttccatagc
    14161 tccaaatgaa catacctaag tacacttccc aatacacaat ttacacactc ttcctaataa
    14221 atcaaaaaca cctttgtttt aaacaactct attgtacttt caaaaacttt atttgtgttc
    14281 tcccacttca attaaatttg aattatcaag actccagccc atccatccat cactataaag
    14341 ggaggtattt tccactccta tgacatttgc atatgttaat acttcagcag cacgccatcc
    14401 acttccacac ataaatataa gctgtttatt cacatctata ttttccttgt cccacatttc
    14461 taaaatctca gatttatttc tcattgtatt atcaatatta cgatattctt ctagtgaaac
    14521 actattacta cctgaatgtc catataaagc tcctggtata cgcccttttt tatcataata
    14581 tgtatatcca gatacttttc ctatatgttc atcccaagta cgattatcta ctagtataaa
    14641 cttatttttt tcttttagtt tttgtcttaa ttcagatgta gtaacaacta attgtgagtt
    14701 aactggtata tctgctccaa aattagtaca tgaatgtttt ggattactaa taaattctaa
    14761 ttcatatcct gctgataacc atgagtttgt tccaccattc aaaactctta catcttttac
    14821 tcctatatat ctcaatataa cagctagtct ataagatgcc ataggtgtaa tacttgaaac
    14881 tattacagta tcatcctttg ttagtccata gtctaaagca aacttagtta agttatcatc
    14941 attatcaagc atccatgttg gaggaggttc tataatatca gtatttacat gaacacttgt
    15001 aggtatatgc ccttttgtat acgattcttc tccccaactc gcttcaatca tttttatatt
    15061 ctttgaatct tcaaatgtct caggaatttt tccatctaat atatctttta ttataaaagc
    15121 aggaactatc atttgataat ttttatatct ttccattgga agattttcat cgtctgccca
    15181 ttgtttaata tcatatttat atagatattt atatcctttt ttagataaat aatctgctac
    15241 aaccaaagca tcttttccat ttgcatcata caatactata tttttatcta aatcaattct
    15301 tttagttttc aatgcttgtt ctagcacttc atcttttcta tctgagtata catttaacca
    15361 attagcagaa aaatctactg ctctttttat atgcccacct ctttttactc catctaactt
    15421 ccagccatta taagcatcat ttaagcgagt atcaactata acccaagcgt tatcttgcat
    15481 tttttcttgt aaaacttcag tagatattgc cttaagatta acaaaataat cttctttctt
    15541 ttctttttta aaatcatttg tcataattta tactcccaat ttaaaaaaat ttttatttga
    15601 aattatttta ctaaaataaa gtaattttct tatactctat tagcttcatt atattctttt
    15661 ataaatagtt ccttatattc ttattttaaa ctactcactt tatcttatca caaggttact
    15721 aataccttgt attttaattt ttctcataag ctattatttt tttatgtctg atattgacta
    15781 tatgtttatt aagtactcac tatattttat atgaactata tttatcgttc cctatacctg
    15841 gtatggtcta tatat
//
LOCUS       DAEPOZ010000074        15830 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.074, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000074 DAEPOZ010000000
VERSION     DAEPOZ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15830
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.074"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            342..797
                     /locus_tag="KR440_001742"
     CDS             342..797
                     /locus_tag="KR440_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBH0698795.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            846..1430
                     /locus_tag="KR440_001743"
     CDS             846..1430
                     /locus_tag="KR440_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HBH0698796.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSREQMDEVKLAMGFKL"
     gene            complement(1435..1758)
                     /locus_tag="KR440_001744"
     CDS             complement(1435..1758)
                     /locus_tag="KR440_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0698797.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLESL"
     gene            1910..2428
                     /locus_tag="KR440_001745"
     CDS             1910..2428
                     /locus_tag="KR440_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH0698798.1"
                     /translation="MNFVELTKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGRQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(2604..3272)
                     /locus_tag="KR440_001746"
     CDS             complement(2604..3272)
                     /locus_tag="KR440_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698799.1"
                     /translation="MLEISHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            3387..4802
                     /locus_tag="KR440_001747"
     CDS             3387..4802
                     /locus_tag="KR440_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBH0698800.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNSKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQYVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDHEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            5025..5483
                     /locus_tag="KR440_001748"
     CDS             5025..5483
                     /locus_tag="KR440_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0698801.1"
                     /translation="MEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFTNVVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA"
     gene            5480..6064
                     /gene="clpP"
                     /locus_tag="KR440_001749"
     CDS             5480..6064
                     /gene="clpP"
                     /locus_tag="KR440_001749"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBH0698802.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            6386..7462
                     /locus_tag="KR440_001750"
     CDS             6386..7462
                     /locus_tag="KR440_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBH0698803.1"
                     /translation="MIAISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            7529..9568
                     /gene="bclA3"
                     /locus_tag="KR440_001751"
     CDS             7529..9568
                     /gene="bclA3"
                     /locus_tag="KR440_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HBH0698804.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGGTGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGGTGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGP
                     TGATGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAIGATGIGIT
                     GPTGATGATGADGATGVTGPTGPTGATGADGVTGPTGATGATGIGITGPTGATGATGI
                     GITGATGLIGPTGATGATGATGPTGVTGATGAAGLIGPTGATGVTGADGATGATGATG
                     ATGPTGADGLVGPTGATGATGADGLVGPTGPTGATGVGITGATGATGATGPTGADGLV
                     GPTGATGATGADGVAGPTGATGATGNTGADGATGPTGATGPTGADGLVGPTGATGATG
                     LAGATGATGPIGATGPTGADGATGATGATGPTGADGLVGPTGATGATGATGPTGPTGA
                     SAIIPFASGIPLSLTTIAGGLVGTPGFVGFGSSAPGLSIVGGVIDLTNAAGTLTNFAF
                     SMPRDGTITSISAYFSTTAALSLVGSTITITATLYQSTAPNNSFTAVPGATVTLAPPL
                     TGILSVGSISSGIVTGLNIAATAETRFLLVFTATASGLSLVNTVAGYASAGIAIN"
     gene            9737..10498
                     /locus_tag="KR440_001752"
     CDS             9737..10498
                     /locus_tag="KR440_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBH0698805.1"
                     /translation="MKNKIETSMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKMGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            10807..11085
                     /locus_tag="KR440_001753"
     CDS             10807..11085
                     /locus_tag="KR440_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698806.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGEYIYIPRKSNNKKEWGSSTS
                     TREDLSIRNMQIYEDYQVGYDLKYLSEKYYLSLKSIQRIVLQEKRKDS"
     gene            11378..11953
                     /locus_tag="KR440_001754"
     CDS             11378..11953
                     /locus_tag="KR440_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HBH0698807.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPETLEQFHTNDYNLAKQYAMNTELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
     gene            12391..13101
                     /locus_tag="KR440_001755"
     CDS             12391..13101
                     /locus_tag="KR440_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0698808.1"
                     /translation="MISIGICDDELHYRIKIKDILSEILSSYPINYNIYEFSSGEELL
                     NNYPKDLDILIMDIQMKTINGMDTARKIREFDHKLEIIFVTSFVEFMQEGYEVKAYRY
                     ILKPINKEKISKSVLPCINEMMKKRNNYLTINVKNYVDRIKIDSITYIETDRPNILIY
                     THDDMYITKMSISKIEKILNEYGFFRCHNSYIVNLKLVESMSGSTVIVDGKSIPISKY
                     RVKGLKLAITNILGDIVC"
     gene            13095..14411
                     /locus_tag="KR440_001756"
     CDS             13095..14411
                     /locus_tag="KR440_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBH0698809.1"
                     /translation="MLTNLSIGFWNIINLVSGTIDWIIFFLVIHLVGKRKISNIRYIF
                     TTIIIFIFMAYINVVKVFPNIKILLCMVIGISFYITSYEDKIYKSIIVSLLFWLGLMI
                     AEGSAVGLVVFINKLNNINITQHENLFRIQAIIMSKVFLFIGFILFKYFKLSLDFKPK
                     DMILIGIPILSNIVSLLLIFEYNLRVNTITTENLFIFIFAILLILSSSIILLIVIGKI
                     VQDDKLNLEYELINERIKTNYRNYENINEVHDRLKYVYHDLKNHMICIKNYDTKEEII
                     SYIDNLEFQISEFEHFKNTGNKTLDLILAEKISICKKYDIQIEDNINISKLNFIENND
                     ICSIFANSLDNAIEACMDINNELEKRIEVKATYINKFAIIKFINTKVNDIKLIDKRIQ
                     TSKDDDKIHGIGLASIKYIVNKYGGETIVNYSDNEFILKIMIPIKS"
     gene            14423..14563
                     /locus_tag="KR440_001757"
     CDS             14423..14563
                     /locus_tag="KR440_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBH0698810.1"
                     /translation="MKKIALNLLKNISALSFGIAVLSANSASSWVAHQAKEPQALQKL
                     KK"
     gene            14622..15203
                     /locus_tag="KR440_001758"
     CDS             14622..15203
                     /locus_tag="KR440_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBH0698811.1"
                     /translation="MFKRLSYKFANILVNNEIVESEDFEIYRYGFETLIYFIVNISVA
                     LFIGIIFDRFIHTVIFLSCYCTLRQFTGGYHARNYKECTLTFAVIYLITIFSANNIDI
                     NKYKYLLVLLMIISILTIYKLAPLEHRNKPLSESEKKHYRKTVQKILFVIICLIILCK
                     ILNIFQQYVIYAIISIYWIAILIYIGMKVNNDQ"
     gene            complement(15612..>15830)
                     /locus_tag="KR440_001759"
     CDS             complement(15612..>15830)
                     /locus_tag="KR440_001759"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698812.1"
                     /translation="SIKLFIITEINDITIDKIVSGKNHSNTNINIVDLIVTIDNVLIK
                     LEYAIAYIIGMQQNIKLFLVTATEIPKK"
BASE COUNT         6186 a         1830 c         2738 g         5076 t
ORIGIN      
        1 gctaccaata aaattttata gtctatggga aaattaagat tatgagtttt atacttttga
       61 tatagggata ctattttcat aaatattaca acatgtagaa gtatatgtct agtattgttg
      121 aattttcaat gatatattaa ggttctaata tattatacaa aataaaaaca ttaattcaac
      181 ttaaataatt ttaagtatta tatgtgtttt gattaaagaa aactaaaatt ttaaggaaaa
      241 ataacatatt tgtattaaat tttattgaaa tgattaaatt tatttactta atgtaaatta
      301 ttagctaaaa ttgtattaaa taattaagga ggtgagtagc tctggatgaa atagatttaa
      361 aaataattaa ttctctaaaa aagaatagta gggcttcaac ctctgaaata agcagacaaa
      421 ttaatttatc agtaccagca gtctcagagc gtataaggaa aattgagcag tcaaatttaa
      481 ttcaaaagta cactattcaa attaatcggg aaaaaacaaa atataagctt ttggctttta
      541 tatttatcaa tactgattta tctaaaagta tgaaagaatt taagaaaact atattggaat
      601 atgatactgt attagaatgt catcatattt caggagagta tgattactta ataaaagtat
      661 tagcagaaga tacaagtaat cttgaagagt ttatatcagt gactttgaag gaaattgggg
      721 ggattcaaaa agctaataca accattgttt tatctacaat taaagaagaa tttaatacaa
      781 taaaagatat tttttaaata atttactagt aaagtgttaa ttatatttta ggaggaaaat
      841 agaatatgaa agttttagtt tttttagcaa aaggttttga aactatggaa tttagtgtat
      901 ttgtagatgt tatgggatgg gctcgtaacg attatgggca tgatattgat gtagtgactt
      961 gtggatttaa aaaacaagtt atgagtacat ttaatataca agtattagtt gataagacga
     1021 tagaagaagt atgtgttgat gattatgatg ccctagctat tcctggagga tttgaagaat
     1081 ttggatttta tgatgaagca tatgattcat cttttttaaa tctaataagg gagtttaatt
     1141 caaaagaaaa aataatcgct tctatttgtg tggcagcatt gccagttgga aaaagtggtg
     1201 ttttaaaaaa tcgtaaagct actacatatc atttgaaaaa tggtaaaaga caaagacagt
     1261 taagtgagtt tgatgtaaat gtagttaatg aaccaattgt tgtagataaa aatattataa
     1321 cctcatattg tccagaaaca gctccacatg tagcttttaa gcttttagaa atgttgacat
     1381 ctagagaaca aatggatgaa gttaaattag caatgggatt taaactgtaa tgctctatag
     1441 actttctaag taatcagaac cccagtcaca catggaatct aaaattggaa taatactctt
     1501 tccaaaatca gatagcgagt attctacttt tggaggaaca acaggataaa ccactcttat
     1561 aattaaacca tctgattcta attcacgtaa ttgttgagtt aacatcttag gcgttgcttt
     1621 aggaattaat ttttttaatt cattaaacct caatatagtg tctttaagat gccaaagtat
     1681 aagtgtctta tatttaccac caatcaatgc aagtgttgct tctattgggc aactgtaatt
     1741 atctaaacat tttttcattt ttgagctcct ttatttattt taaagtatct ttttagatac
     1801 tatattacaa aaaagtgcat tattgtaata tagtaccata gtgataaaat tatagcatag
     1861 gttaaataaa tcatcaactt agcctaataa aacaataggg ggtaaataaa tgaatttcgt
     1921 tgaacttaca aaaaaaagat attcttgccg taattatcaa gatagaaagg ttgaaaaaga
     1981 aaaacttgag aaggttttag atgttgcaag aatagctcca actggtggta atcgtcaacc
     2041 tcaaagattg attgttattc aggaaaaaga aggtataaat aaactttcta aggctgccaa
     2101 tatatatgat gctccattag caattcttgt atgtggagat aaagacaaag tttggacaag
     2161 accttttgat gggagacaac ttacagatat tgacacaagt atagttacag accatatgat
     2221 gttacaggca actgaacttg gattggcaag tgtttgggtg tgctatttta atccagatat
     2281 aattagagaa gagtttagtt tgccagataa tctagaacca ataaatatat tattgatggg
     2341 atatgaatct aagataccag agagcccaga gagacatgaa aaaactcgtg ttcctttaag
     2401 tgaaattgtt tcttatgaaa ctctttaatt ataaaattaa gttattcata ggataaatgt
     2461 aataaagtgt ttattataaa caaaattcaa aataaataat atctacatat aattaatttt
     2521 agtgaagtat atctagttta actagaatgt atacttcact aatttattta ttcaaaatat
     2581 ttatgaggat tttaatatat taactaaaat atatcaattg aattaacttt taaatctgga
     2641 aaagcacttg aaatatagtg ttttatgtca tttaaatggt cttgaatttg cttttctgat
     2701 attttatcag gtccatatac aaaatacata atatttttag tatcttttgt tatagctgtt
     2761 cgattatcag ggccattaag aatactagat aatataccaa tattatctga taaaaataaa
     2821 tcatcttttg ttaaaatatg ctctttacca cctataccat aaaaatgttc acttccattt
     2881 gctaattcta ttttcaaagg taattccatt ttatcaaggt catgacctgc tgttagaaga
     2941 agatttttca attctgctaa aaacattgac tcaattgtta cacctacatc tgggatacct
     3001 tttgatttta atagaataga ttctaattga agcaatacag ggtaagtttt cttgaatttt
     3061 ttataatact ttatataact tgaaacaggc tctgttttaa ttttttcttt acgattgtat
     3121 tctgggtgtt gttctataat atttttaatt gtagagtttt taagaactaa aaaattttct
     3181 ttattaggtt ttgaatgtgt aatatttata accataactc caaatttaat attgggataa
     3241 atttctttaa ctttatgtga tatttctaac ataatataga ctccttttta ttttatattt
     3301 tatgatataa tcttagcaag ttaaaagtgg attttgtagt tccattttta tagtaatttt
     3361 atagaaccac ttttggaggt attagaatgt ggattacgat taatagaaat agtaatatat
     3421 cattatcaag acaaatttat tgtgagataa aagatatgat tctggatgga agcttaaagt
     3481 ttgagcaaaa actgccatca tctaggactt tatcaaaaga actatctgta tctcgtaata
     3541 cagttttaga tgcatataat caattaattg cagaaggata tttggaaaca aggaatggat
     3601 atggaacaat tgtagctaaa ggaatatcag ggcataaagt tgattttaag ttaaatgata
     3661 cttttagaga tgaaaaagat ttacatacta ataaacaaaa aaattatata gattttcgtt
     3721 caggagttcc agaattaaaa ctatttccac gcaaagaact ttcaaagcta tataaacaaa
     3781 tactgtatga tttatcagat tcggatttca gatatggaag tacagctggt gtatgggttt
     3841 taagagtgga aatatctaag tatttatttc gtactagagg aattagatgt aattccaaaa
     3901 acattatgat tgtttctggt tcaacacaag gtttatcttt aatttcacag cttttataca
     3961 aaagtaataa aaaggttgct gtagaagacc ctattcataa aggcttatta aaggttattt
     4021 catctattgg atattcagtt gttggaatta aagtagatga taagggaatg aataccaact
     4081 taataaaatc aataaagggg atttcttttg tttatactac accttctcat caatatccat
     4141 taggagggat tttacctatt caaagaagac tagagcttat taaatgtgca acagagaatg
     4201 attactatat aattgaagat gattatgata gtgaatttag atatgagggt caaccaatta
     4261 gttctcttta tgaacttagc ccaaataaag taatttacat aggttctttt agtaaaatat
     4321 tatctccagc tattcgtgtt ggatttatgt tattaccaga caatatttta gaaagctata
     4381 aaaccttaaa aatgtactct gatgttcata cagaaacaat attgcaatat gtattagctg
     4441 aatttattgg aaatggaggt ttagagaagt acatatttag aatgaagaag ttgtacaata
     4501 gaaaacgtaa atatctcata aaagttttaa acacggaatt taaaaatgaa tttaaaataa
     4561 aaggtgaagc agcagggctt catatagttg ctcatttcta taatgttaaa tttacggaaa
     4621 gtttaattca taaaattttt gaagcaggta taaaagttta tcctatacaa caatatgcaa
     4681 ttgagaatat attagaatat gaccatgaaa ttatactagg ttatgctcat cttagttttg
     4741 atgaaattga aaaaggtgta aaaatactca gcaaaataat acatatgaat caaggtacat
     4801 agataaaaca tgtagcaatc tacatataat agatgtaatt aagtttaaac tttaaaagaa
     4861 atatatatta atatttttgt ataaaaatac ttgatattgt tttatcgata ctttattgct
     4921 atacactaaa ttaagatagc aatttatatt aagaaaaaac tttttgcata ggttataata
     4981 cttaaaagga gaggatagag ttgaaaacaa atacagatgt tattatggag ataattgatt
     5041 atatagaaga aaatcttaca gaaaatctaa atttagataa aatagcttat attgctaatt
     5101 attcaaaata ccatttacac agaatgttta caaatgttgt aggatgtact atccatcaat
     5161 acatacagag gaggagactt actgaagcag ctagacaact tatatttaca gacaaatata
     5221 ttatagatat agctattaca gtaggatatt ctacacatca atcttttaca ctagcattta
     5281 gaaatttata taaagaatct ccacaagtat atagaaataa agatgaattt tatcctattc
     5341 aacagagatt tgttttaaat ctaaatcatc aaattcaaag cgaaaaatgt atttcttatg
     5401 caaatatgaa ttataaacaa agaagagatg aaatcaattc ttatcttaac tttaatatta
     5461 attgtgaggt gaaagtagca tgagtttagt gccacatgta attgaacaaa caggtcaagg
     5521 ggaaaggtct tatgatatat attcaaaatt attaaaagat agaattatat ttataggaga
     5581 agaaattaat gatgctattg caagtttagt agtagctcaa ttactgtttt tagaatcaga
     5641 agacccagat tcagatataa ttatatatat aaatagccct ggtggctctg ctacatcagg
     5701 atttgctata tatgatacta tgaattatgt cagatgtgat gtatcgacta tctgtattgg
     5761 tatggctgca agtatgagtg cttttttact tgcaggagga acacatggaa aaagatgtgc
     5821 tttacccaat tcagagatta tgattcatca gccaatggga ggagctaaag gtcaagcaac
     5881 agatgtaaaa atagctgtag ataatatatt gaaaataaaa gaacgattag ataaaattct
     5941 ttctgaaaat acaggtaaat caatagaaga tattagaaga gatacagata gagataattt
     6001 tatgactgca ctagaggcta aagagtacgg gctgattgat tatataatga ataggaatga
     6061 ataggaatga ataaagtatc aaagtatcat attaaaatgt ctgttttaca aatatatata
     6121 taatattaaa taagagtgtc tcaaaatgaa ctttttagtt tatgggacac tcttttttgt
     6181 atgaaattaa atatggtttt atcatttcaa caattattac caaatttcaa gtttaataaa
     6241 ttctactata aaaaatacat aaaagataag gttctttagt aactttctgt cgtcaaaagt
     6301 ttttataaat ttaataacaa aacaagagaa aataccatat attacttatg tctgcaatat
     6361 atagaagaca aaggagttgt acattatgat tgcaataagt ttgtgcatga ttgttaagaa
     6421 tgaggaagaa gttataggca gatgcttaga atgtgtaaag gatatagtag acgaaattat
     6481 aattgtagat accggttcta cagattccac aaaagaaata gtctcaaaat atacagacat
     6541 ggtgtacgat ttcgaatgga tagatgattt ctctgctgca agaaattttt cattttctaa
     6601 agcttctaaa gactatacta tgtggcttga tgcagatgac ataattttag aagctgatag
     6661 agaaaaatta ttaaagatta aagaaagctt ggatacttcc attgatattg ttatggcaaa
     6721 atacaatgta tcctttgatg aaaatggtaa cccaactctc tcatattata gagaacgatt
     6781 gtttaaaaga agttctaatc ataaatgggt tgaccctatt catgaagtag tacctctttt
     6841 tggagaggta ttttattctg acattgcaat atctcataaa aagcttcata gacaggaccc
     6901 tctaagaaat cttagaattt ttgaaaaaat gatttcagaa ggaaaaacat tggagccaag
     6961 acaccaattc tattactcta gagagttgta ctataatgct aggtataaag aagcaataga
     7021 aggttttact aagtttttag actcatcaag aggatggatt gaggattgta tcagtgcctg
     7081 cagagattta gccacatgtt attacttaat taatgatgaa aaaagtgcct tatattcatt
     7141 gtttagaagt ttcgaatttg atgagccaag agcagaaatt tgttgtgaca taggaaagca
     7201 tatgtttgat agacaaaaat ataaagaagc cattttttgg tataaggtag cattaacaag
     7261 agataagaat gatactaatg gaggttttaa gtcaaatgat tgttatggat atataccatc
     7321 tatacaatta agtgtctgtt atgacaggtt aggagaatca gataaagcta tttattatca
     7381 tgaaaaaaca aaagaaatca aacctaacga tagtgctgtt ttgcacaatg aaaattattt
     7441 tagtaaattt aaaaatagtt gataggtcac aatcatacta aatttgacat attatatata
     7501 gagcttaaaa gctcaaatat atcaggaggt gcttttaata atgagtagaa ataaatattt
     7561 tggaccattt gatgataatg attacaacaa tggctatgat aaatatgatg attgtaataa
     7621 tggtcgtgat gattataata gctgtgattg ccatcattgc tgtccaccat catgtgtagg
     7681 tccaacaggc ccaatgggtc caagaggtag aaccggccca acaggaccaa cgggtccaac
     7741 aggtccagga gtagggggaa caggcccaac aggaccaacc ggtccgactg gcccaacagg
     7801 aaatacaggg aatacaggag caacaggatt aagaggtcca acaggagcaa cagggggaac
     7861 aggcccaaca ggagcgacag gagctatagg gtttggagta acaggcccaa caggcccaac
     7921 aggagcgaca ggagcaacag gagcagatgg agtaacaggt ccaacaggtc caacgggagc
     7981 aacaggagca gatggaataa caggtccaac aggagcaaca ggggcaacag gatttggagt
     8041 aacaggtcca acaggcccaa caggagcaac aggagtagga gtaacaggag caacaggatt
     8101 aataggtcca acaggagcga caggaacacc tggagcaaca ggtccaacag gggcaatagg
     8161 agcaacagga ataggaataa caggtccaac aggagcaaca ggagcaacag gggcagatgg
     8221 agcaacagga gtaacaggcc caacaggccc aacaggggca acaggagcag atggagtaac
     8281 aggcccaaca ggagcaacag gagcaacagg aataggaata acaggcccaa caggggcaac
     8341 aggagcaaca ggaataggaa taacaggagc aacagggtta ataggtccaa ccggagcaac
     8401 cggagcaacc ggagcaacag gcccaacagg agtaacaggg gcaacaggag cagcaggact
     8461 aataggacca accggggcaa caggagtaac cggagcagat ggagcaacag gagcgacagg
     8521 ggcaaccgga gcaacaggtc caacaggagc agatggatta gtaggtccaa caggagcaac
     8581 aggggcaaca ggagcagatg gattagtagg cccaacaggt ccaacagggg caaccggagt
     8641 aggaataact ggagcaaccg gagcaacagg agcgacaggt ccaacaggag cagatggatt
     8701 agtaggtcca accggagcga cgggagcaac aggagcagat ggagtagcag gtccaaccgg
     8761 agcaacaggg gcaacaggaa atacaggagc agatggagca acaggtccaa caggggcaac
     8821 aggtccaaca ggagcagacg gattagtagg tccaacagga gcaaccggag caacaggatt
     8881 agcaggagca accggagcaa caggcccaat aggagcaaca ggtccaacag gagcagatgg
     8941 agcaacaggg gcaaccggag caacaggtcc aacaggggca gatggattag taggtccaac
     9001 cggagcaacg ggagcaacag gggcaacagg tccaacaggc ccaacaggtg ctagtgcaat
     9061 aatacctttt gcatcaggta taccactatc acttacaact atagctggag gattagtagg
     9121 tacaccaggt tttgttggct ttggtagttc agctccagga ttaagtatag ttggtggagt
     9181 aatagacctt acaaacgcag cagggacatt gactaacttt gcattttcaa tgccaagaga
     9241 tggaacaata acatctattt cagcatactt cagtacaaca gcagcacttt cacttgttgg
     9301 ttcaacaatt acaattacag caacacttta ccaatctact gcaccaaata actcatttac
     9361 agctgtacca ggagcgacag ttacactagc tccaccactt acaggtatat tatcagttgg
     9421 ttcaatttct agtggaattg taacaggatt aaatatagca gcaacagcag aaactcgatt
     9481 cttactagta tttactgcaa cagcttcagg tctttcatta gttaatactg tagcaggata
     9541 tgcaagtgca ggaattgcaa taaattagat tagataagaa aaggcttcta atatcgtatt
     9601 gatattagga gcttttttaa gaatgattga tgaacatagt tttaatatta attagtgcgc
     9661 atattataaa atactattat ataatataaa ataataacat taaattggaa atatagaatt
     9721 atctaggggt gatactatga aaaataagat tgaaacatca atgaacaact ttgtaaaaaa
     9781 ttatcataaa tcaaataata taactacaaa gtggcaagaa cttttgtttg cttatgcatc
     9841 tgctgaagat gatatgtttc agaagctaaa aagtgttgta ggaacatctc atgctatgcc
     9901 aaaagatttt ctggaaaatg gaaaaactat aattacatat tttatacctt ttgatgaaag
     9961 tgtaaataat agtaatattg gagggtttga atgttctgaa atatgggcaa gagcgtacat
    10021 agaaactaat atgttgattt caaatttaaa tgatttctta tgtgaagaac tagagaagat
    10081 gggggctaat tcatatgcta ttcctgggtc gtataatttt gacagtgaaa aattggtaag
    10141 tgactggtca caaagacatg tagcattcat agcaggtctt ggaacatttg gtttaaataa
    10201 tatgcttatt acagaaaaag ggtgttgtgg aagaatagga agcataatca caagtttaga
    10261 attagagcca actaaaagac cagaaaatga atactgcttg tataaacata ataatagttg
    10321 caaaaaatgt gtcaacaaat gcgtgaaaga agctttaaaa gaagatggat ttaataggaa
    10381 taagtgttat gaaatgtgta tgataaatga aaaaaaatac acttatatag gtggttgtga
    10441 tatttgtgga aagtgtatag ttggggttcc ttgttcggta ataaatccct caagataaag
    10501 gacttatgtt gattaataac tttaaatata tctaataaga agaaaaaagt tagtctctaa
    10561 aatagatgaa ttatgaaaat aaataaagat aatataaagg ttagatgaag aagttatgtt
    10621 aattgacaac tatttatact gctgatagac ttatatgtat cgttgcaata tataaggagg
    10681 ggtgagattt atgtttgatt taatctttat ttttgcatag caagggctat tgcaaatata
    10741 aaatttaata tataaatagc cctttgctat tcctaataaa aaattattat ttaataggag
    10801 tgcagaatga gctataaaaa agcaatacat atattaccag aagaattact tgaattgata
    10861 caggaatatg tagatggaga atatatctat attcctagaa agtctaataa taaaaaagaa
    10921 tggggaagta gtacgtctac tcgtgaagat ttatcaatta gaaatatgca aatttatgaa
    10981 gattatcaag ttggatatga tttgaaatat ttatcagaaa aatattactt gtctttgaaa
    11041 agtattcaaa gaattgtatt acaagaaaaa agaaaagata gttaagaatg agccagtata
    11101 gttttataat tatactggct cattttcttt tgataaaagt gtttgaggta tagtttctta
    11161 taagtggatg ctataattta aataatttca ataacttgta cagcagataa cgaatagagt
    11221 tgtaaagtat ctaataagaa gtagtagctt tttatatgtt tgaaattaaa atttattaaa
    11281 attaggaggt cttgttttga aaaaaatact tgattatatt ccaccttttc ataactttct
    11341 attaatttag taatcttata gaaaggaagt tgcatttatg aaaaaaatta cttgggaggt
    11401 ggaatatatc tcccagttac ctgtaattag gtattgtaag aaatgtggaa aaaagactga
    11461 atatatttgt tcaggtttat ttagggttaa tgctcaacat aaatatttgg atatatggtt
    11521 aatatacaag tgttcaaatt gtgattcaac atggaactca actatatatt ctcgtgtcaa
    11581 tccaaaaagc ttaaatccag aaacactaga acaattccat acaaacgatt ataatcttgc
    11641 taagcaatat gctatgaata cggaactttt acatagaaat ggagcagaag ttggtattcc
    11701 agagtataaa atagtaggac aagaaattaa tattaatgac cttacacaag ttcatattat
    11761 tagtaaatat ccatgtcagt taaaggtatc aacattatta cgtgagaaac taggtttatc
    11821 taggaaggta tttgacgaaa tgttggattg tgggattatt agaagtaact caggagttga
    11881 tttaaagaaa ggtaaattaa attatgaaat tatgttagat ataggcaagg agaagcaaaa
    11941 gtcttcaaat tgattagaat aactaactta atatattttc aggaacatca tttaaaaaaa
    12001 tagtggcttt aaaaaattta aagccactat tttttaatat gttttatatg aatatttaaa
    12061 atatagtgtg tattcttcaa aattatagcg ataattaaaa tattactata aaaaggaagg
    12121 tgcatacttt ttgtttaaca aaaactagat actaaaaact tctcgaagaa tatgttatta
    12181 aaatcgctaa atatattcaa tatatattaa aaaattgtat tataatgaaa attaaaatag
    12241 attgtaaatc caaaaagtaa tacttaatgt ccaaaaagct aaaattaaaa tctaaatagc
    12301 tatttgctac agaagtatgt taaaatatgt aaaatgaata gaaattttat atatttatta
    12361 aaaatatata agaaaggatg tttaagaatt gtgattagta taggaatatg tgatgatgaa
    12421 ctacattatc gaataaaaat aaaagatata ttaagtgaaa tattaagttc atatcctata
    12481 aattataaca tatatgaatt ttcatcagga gaagagttac taaataacta tcctaaagat
    12541 ttagacatat taattatgga catacaaatg aaaacaataa atggtatgga tactgctagg
    12601 aagataagag agtttgatca taagttagaa ataatatttg ttacttcatt tgtagagttt
    12661 atgcaagaag gttatgaggt aaaagcatac agatatatac taaagccaat aaataaagaa
    12721 aaaatttcta aaagtgtatt accttgtata aatgagatga tgaaaaagag aaataattac
    12781 ttaactataa atgtaaaaaa ttatgtagac cgaattaaaa tagattctat tacttatata
    12841 gaaacagata gaccgaatat attgatttat acacatgatg atatgtatat tactaaaatg
    12901 agtatatcta aaatagaaaa gatattaaat gaatatggat tttttagatg tcataatagc
    12961 tatatagtta atttaaaatt agtagaatca atgagtggaa gtactgttat agtagatggc
    13021 aaaagtattc caataagtaa atatagagtt aaaggcttga aacttgctat aactaatata
    13081 ttaggggata tagtatgcta acaaatctaa gtataggatt ttggaatatt attaatttag
    13141 tttcaggtac tatagattgg attatatttt ttttagtaat ccacttagtt ggaaagagga
    13201 aaatatctaa tataagatat atatttacaa caataattat atttatattt atggcataca
    13261 taaatgtagt taaagtgttt ccaaatatta agattcttct atgtatggta ataggaatat
    13321 cattttatat aacatcatat gaagataaaa tatataaaag tataatagtt agtttattgt
    13381 tttggctagg attaatgatt gctgaaggtt ctgcagtagg tctagtagta tttataaata
    13441 aattaaataa tattaacata acacaacatg aaaacttatt tagaatacaa gctattatta
    13501 tgtcaaaggt atttttattt ataggtttta tattgtttaa gtattttaaa ttatctttag
    13561 attttaaacc taaagatatg atactaatag gaatacctat attatctaat atagttagct
    13621 tattacttat atttgaatat aatttaagag tcaatacaat tactacagaa aacttattta
    13681 tatttatatt tgcaatttta ttgattttat catcaagtat aatactttta atagtaatag
    13741 ggaaaattgt acaagacgat aaattaaatc tagagtatga acttataaat gaaagaataa
    13801 aaacaaacta caggaattat gaaaatataa acgaagttca tgatagatta aaatatgttt
    13861 atcatgattt gaaaaaccat atgatttgta taaaaaatta tgacactaaa gaagaaatta
    13921 tatcatatat agataactta gagtttcaga taagtgaatt tgaacatttt aaaaatactg
    13981 gaaataaaac attagattta attttagcag aaaaaataag tatttgtaaa aagtatgata
    14041 ttcaaattga agataacata aatatatcta aattaaactt tattgaaaat aatgatatat
    14101 gttcaatatt tgcaaattca ctagataatg caattgaagc atgtatggat ataaataatg
    14161 agctagaaaa aagaatagaa gttaaagcta catatataaa taaatttgca ataataaaat
    14221 ttataaatac taaggtaaat gatattaaac ttattgataa acgtattcaa actagtaagg
    14281 atgatgataa gatacatgga ataggacttg ctagtattaa atatatagta aataaatatg
    14341 gtggtgaaac aatagttaat tactcagata atgagtttat attaaaaata atgataccaa
    14401 taaaaagtta ggagagtaca aaatgaaaaa aatagcgtta aacttactta aaaacatctc
    14461 agcattaagt tttgggatag ctgtattatc agcaaacagt gcaagttctt gggtagcaca
    14521 tcaggcaaaa gagccacaag ctttacaaaa actaaaaaaa taacattaat aaaaggacct
    14581 cttcttatta aaggagaggt tttacatgtt gaggaggaaa tatgtttaaa agattatcat
    14641 ataaatttgc caatatatta gttaataatg agattgtaga aagtgaagat tttgaaatat
    14701 atagatatgg atttgaaact ttgatttatt tcatagttaa tatttcggta gcattattta
    14761 taggaattat ttttgataga tttatacata cagttatatt tttaagttgt tattgcactt
    14821 taagacagtt cacaggaggt tatcatgcta ggaattataa agagtgtaca ttaacatttg
    14881 cagttattta tttgataact attttttcag ctaataatat agatataaat aaatataagt
    14941 atttattagt attattaatg attattagta tactaacaat ttacaagtta gctcctttag
    15001 aacatagaaa caaacctctt agtgaaagtg agaaaaagca ttatagaaag actgtgcaaa
    15061 aaatactatt cgtaataata tgtttaatta ttttatgtaa aatacttaat atttttcagc
    15121 aatatgtgat ttatgcaata atttctatat attggattgc tatattaata tatattggaa
    15181 tgaaggttaa taatgatcag taagattata aaacattttc taaattaatt aatttcacta
    15241 attaatatta attttaaaaa ataatgtcaa ggtaaatatt tgaacttatt aaatatagtt
    15301 gaatctattg taagttgtat caaaggattg gctaggaggg gcataaatat gagtagtatt
    15361 aaggttatat cagactttat aaccaaaata attagaaaaa atattttatt tgcaataaca
    15421 gttattatta taaattacca tctcatacaa cgatggacac atatatccaa tagaggaatt
    15481 tcatttaatg attacttatc cttgagtgta tatacttgta tgacaattag tattttaacc
    15541 atgttaaatg taatttttta tatgagaaaa agttatgata ctgtaaaaat attatgtaga
    15601 aataattttg attatttttt aggtatttct gttgctgtaa ctagaaacaa ctttatattt
    15661 tgttgcatac caattatata tgctatagca tactcaagtt taataagaac attatcaatt
    15721 gtaacgatta aatcaactat atttatattg gtatttgaat ggtttttacc acttactatt
    15781 ttatctatag tgatatcatt tatttcagtt attataaata atttaatact
//
LOCUS       DAEPOZ010000075        15704 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.075, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000075 DAEPOZ010000000
VERSION     DAEPOZ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15704
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.075"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(13..219)
                     /locus_tag="KR440_001760"
     CDS             complement(13..219)
                     /locus_tag="KR440_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0698813.1"
                     /translation="MFKKKSGYTFTNFLNKVRVEKNKKYLLKKDLSILDVTVLIGFNS
                     QNYYSIIFKKFNNLTPIEYKNIYN"
     gene            524..715
                     /locus_tag="KR440_001761"
     CDS             524..715
                     /locus_tag="KR440_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698814.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            complement(804..1616)
                     /locus_tag="KR440_001762"
     CDS             complement(804..1616)
                     /locus_tag="KR440_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HBH0698815.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            2038..3189
                     /locus_tag="KR440_001763"
     CDS             2038..3189
                     /locus_tag="KR440_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0698816.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDDDLDKMLELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLEYLNYKVHVKNLKDQLKCNG
                     TESLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
     gene            3307..3945
                     /locus_tag="KR440_001764"
     CDS             3307..3945
                     /locus_tag="KR440_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBH0698817.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            3946..4968
                     /locus_tag="KR440_001765"
     CDS             3946..4968
                     /locus_tag="KR440_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HBH0698818.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLETLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEYIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRGYDNFLCDFGCNCLNTTPVENLQAAIKAGRINYKELDR
                     IDIDEL"
     gene            4958..5578
                     /locus_tag="KR440_001766"
     CDS             4958..5578
                     /locus_tag="KR440_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698819.1"
                     /translation="MNYNSNKNKYLLKDINLEDIFIDKNIVLKFLGYGNRKAPDTISE
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDIKMNDYMENCNM
                     MAGIALDKIGIVCLDYINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(5702..6388)
                     /locus_tag="KR440_001767"
     CDS             complement(5702..6388)
                     /locus_tag="KR440_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBH0698820.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQKKRLENSKIKGYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALMIGNSLTSDVLGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(6402..6947)
                     /locus_tag="KR440_001768"
     CDS             complement(6402..6947)
                     /locus_tag="KR440_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0698821.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCIDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            7175..8422
                     /locus_tag="KR440_001769"
     CDS             7175..8422
                     /locus_tag="KR440_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBH0698822.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLAFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSSGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            8509..10503
                     /locus_tag="KR440_001770"
     CDS             8509..10503
                     /locus_tag="KR440_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBH0698823.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIKNTFSENTN
                     KTSEIEIYNKILKINTFPVEYKKNNTEYIVVLIKDITNEKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIRNFIENIVKLQYKMLQLKNVEIKIDCEHNLVCYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNHCLFIDFKDNGEGIKEDALKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            10503..11825
                     /locus_tag="KR440_001771"
     CDS             10503..11825
                     /locus_tag="KR440_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBH0698824.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNFEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            12104..13390
                     /locus_tag="KR440_001772"
     CDS             12104..13390
                     /locus_tag="KR440_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBH0698825.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            13476..14783
                     /gene="grdB"
                     /locus_tag="KR440_001773"
     CDS             13476..14783
                     /gene="grdB"
                     /locus_tag="KR440_001773"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:14523..14525,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBH0698826.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVVTGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
     gene            complement(14999..15223)
                     /locus_tag="KR440_001774"
     CDS             complement(14999..15223)
                     /locus_tag="KR440_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBH0698827.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(15320..>15704)
                     /locus_tag="KR440_001775"
     CDS             complement(15320..>15704)
                     /locus_tag="KR440_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HBH0698828.1"
                     /translation="FKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
BASE COUNT         6262 a         1662 c         2610 g         5170 t
ORIGIN      
        1 gaatgatatt ttttagttat aaatattttt atattcaatt ggtgtaaggt tattaaactt
       61 tttaaaaatt atactatagt aattttgact attgaatcca attagtacag taacgtctaa
      121 aatagacaag tcttttttta atagatactt tttgtttttt tcaactctaa ctttatttaa
      181 aaaattagta aaagtatatc cacttttttt cttaaacaaa gaaaaaaata acttttatta
      241 agttgtaatc cattacatat attacttact gttatatctt ctgagtagtt tttgtgaata
      301 taatctatag ttttctttaa gttttcttta cataaaacta agagttggtc tatgtttcat
      361 tttatcaagg catatttatt aaaaaatcaa tctctatttg ttaaaataat ttatgaatga
      421 tgttagaaac tgtaatatat tggatataat ataataaaca gataaactct gtattaagag
      481 gatatatagt ttatcttaat tataggggaa gtgaggatat attatggaaa gattaatgga
      541 actcatagaa agaaaatttt taaaggatgt agaactttta gaattagaag acatagaaaa
      601 tgtaatagaa tgcaaaatga cacagccata taatgctcat tataatgaat acttagtaaa
      661 agtaaaaaat ggagatgaag tagaagaata ttttgtgtat ttaaaacata gataaacata
      721 aaagaggttt atatttattt gtatgattat aaaataaata caagcctctt tttttgataa
      781 atataaaatt ataaaatttt tatttattta attttttcaa caacattagt caatattgaa
      841 tctaaatcat cactaatttc tgtaactcca ttatgagtaa tatctataat ttctttagtc
      901 ataggaagtt ctcctaaaag ctctaagtct aaatcatcta aaaacttttc agtagattct
      961 ccttcaaata acttgatttt tttagaacaa tcaggacatt ggatataact catattttca
     1021 ataactccaa ggacatttat gttcattttt ttagccatat taacagcttt tgatacaatc
     1081 atagatacca aatcttgagg tactgaaacc ataactattc cacttatagg aatagattgc
     1141 ataaccgtta aggcaacatc tccagtacca ggaggcatat ctattaataa atagtctaat
     1201 tcttcccaaa gtacatcagt ataaaattgc tttacaactc ctccaagtaa aggtcctctc
     1261 caaacaacag gttcattctc atcatctatc ataaggttta tagacattac ttttatgtta
     1321 tttgagtttt ctacagggta aatataagag ccatcagaat aagcttttac attttttaca
     1381 cccataagtc taggaatact tggtccagtt atatctgaat ctaatatacc aaccttatat
     1441 cccattttgt ttagcttatt agcaagtaaa gcagttacag ttgattttcc aactccaccc
     1501 ttcccactca taacaccaat tatttttcca aatttattgt tagggttatt ttcaatagaa
     1561 caatttgatt gactattaca atttcccttt gatggacatg aattacaatc tgccatatct
     1621 attacctcca aactttaata tttattagat tatttgtaac ctacatatac tattttagta
     1681 caattgaacc ataaagtcat tatttaaaag caacttagaa gattttctat tgatttattc
     1741 atagactata gcaaaaagaa tacaataaaa aaattataac attcttgaaa aagtgattat
     1801 ttcaatttag ttatagaact tagtcatata aatatataga aaatatttta atatattata
     1861 cataacgggt tattttataa gtttaactat aaattaggtt tatattttat aaaaatttgt
     1921 taaaaaaaac aattaaaaat tgtaaagaaa aaagatataa ttagaactgt agtaataaat
     1981 gttttattat agataataga gttttaagta tcttaaataa tatttggagg aatatgaatg
     2041 gaattaagac caagaagtgc aaaattagaa gaatttgata aagtaataga attaataaac
     2101 tatgtattta gaatatcaag aaatcataag ccaactatga tggaagaatt tcctctttta
     2161 ctaagtaaaa ataatataga aaatatgatt ataatatcag aagatgataa agtggtttct
     2221 gatgtaaact atctaattca agatgtatct attcaaggaa atagattgaa ggttgcagct
     2281 ataggtggag tatgtactca tccagattat gaaaagagag gctattcatc taagatattg
     2341 gataaagtag aagaaaaaat gttttatgat ggtgtagata ttgtaattat atcaggaaca
     2401 agaagtctat acagtagaag aaattgtagt ttggtaaaga gtttttataa atatacaata
     2461 aaacctgaag atgtaaaaat agcatatgaa atagttgaat ttgatgaaac taattttgaa
     2521 aaagatgatg atttagataa aatgcttgaa ctatataatc aaaatagtac tagatttata
     2581 agaacgagag atgagtttca aaaactattg catgcagcaa ctattgcctg gggaccaatt
     2641 ggttacaaga aggtatttat aaaggaaaat aataatataa taggatattt gataataaga
     2701 acaataaaaa aagaagattc tactgtagga gaagttgctg aaatagggtt aaactctgta
     2761 aatgttgaaa atatattgaa atatgttgca aataaatttg gattagaata tttaaattat
     2821 aaagtacatg tgaaaaatct taaagaccag ttgaaatgta atgggacaga atctttggac
     2881 tatcaacaag gaactatgaa aataataaat tttactaagt tgtgtgatag tttaagaagt
     2941 tattttagtc aatatgtaga ttttgaatta ctaaaataca tggaatttaa acaagttgaa
     3001 aataaatata ttattaaata caaagaagaa gaacttgtaa tagaaaattt ggataagctt
     3061 aataaactgt tttttgaaaa aaatgaggaa cagtataatg aatttaaaca tctaaaaaat
     3121 atatatgaat ttgctacaaa ggcatttcct gtagattttc catggactgc aaatttaaat
     3181 tatcaatagt tttagatgat attaaataaa attgattgat acctagagtt agtccttgtt
     3241 aaataagaga aattattgga tattgaaaaa gcaattttgt aagtaattag aaaggatgag
     3301 tattatatgg acatacttga tgatatagca gaatgtatat taaatatggg tgtagataat
     3361 atagagaatt tagtaaaaat tgctatagat aaaaatattg atgtggaaga tatttatgaa
     3421 tatggtttaa acaaaggtat gataggagct ttagataagt ttgagaataa ggaatactat
     3481 ctttcagaag ttatagtatg cacagatgca ttaaataaag gtattaatct tttgaaggga
     3541 actggaaaag taaagaaaaa atcaaaggga gtaattctga tgtctgtagt agaaggagat
     3601 actcatgaga taggaaaaaa tattgtaaaa gtaatggtag aagccactgg atataaggtt
     3661 attgacttag gtgtaaatag aaaaagtgaa gatataatag aagaagctat aaaaaacaat
     3721 gtagatataa taggcttatc ttcaatgatg acaactacaa tggaaaatat gaaatcggtt
     3781 atagacgagc ttaatatgat tgagatagat aaaagaccaa aagtaataat tggtggagga
     3841 cctgtaagta tggagtttgc agaagaaata ggagcagatg gatacagttc aaatgctcca
     3901 aaagcagtta aattaataaa caaacttata ggaggtgaag tttaaatgtt ttcagatatg
     3961 actttacttc agtatataaa tagtgaagat agaggttttt ttctgcctga tatgggtaca
     4021 aatggattgt tcttaataaa taaaaaggca tatgaagtat atgatagtgt agatttgcaa
     4081 ttagaattag caaagactat ggacaagtat tttgaatcag attttatata ctcattctgt
     4141 gatgggatta cattttgtga aactctgggt ttagagacat taagaccaga ttatgatttt
     4201 ccaagtgtat tgacacatac tataactgac agagataaat taagaaaatt agatgttcca
     4261 gacccttata cgagtggacg tatgccactc aatatagaga gcttatcagt tatatcaaaa
     4321 agtattaaaa aaccactata tgaatcgata caaggtccat ttacacttgc aggtcaatta
     4381 gctggagcta ctcaactttt aagatgtata ataactgata aaaaatttgt agaagaattg
     4441 ttggaattta caactgaatt ggtaagaaga tatgctgtag ctgcaaataa agcaggagca
     4501 aaatatattt caatagcaga gccaacgtca gtaaccctaa gtaaagatag atttgatgaa
     4561 tatatagtaa aaaatttaaa taaaatctac aatgaacttg attgttggaa aggtatgcat
     4621 atatgtggag acaccagaga attattagat aatatgctaa gttgtaatat agatgcagta
     4681 agtttagacc aaattttaga ctatgaagaa attgctccaa tgattcctag agatatagtg
     4741 ttgattggta atttagaccc aataaaactt cttggacgtt ctaagccaga taagattaga
     4801 agagagacac taaaattgtt aaagaagatg agaggatatg ataacttttt atgtgatttt
     4861 ggctgtaact gcttaaatac cactccagta gaaaatttac aagcagctat aaaagcagga
     4921 agaattaatt ataaagaatt agataggatt gatattgatg aattataata gtaataaaaa
     4981 taaatatctt ttaaaggata taaatttaga ggatattttt atagataaga atattgtgct
     5041 aaagtttttg gggtatggaa atagaaaagc tccagataca attagtgaaa tcgttgaaag
     5101 agaaataaga aatataaatg atattttaga tatcaagata tatataagtg aagttgatat
     5161 taataaaaca cctaaagaat gtaaaaaagc atttgctgta ctatatacaa taggggataa
     5221 aattgatatt aagatgaatg actatatgga aaattgcaat atgatggcag ggatagcatt
     5281 ggataaaata ggtatagtat gtttagatta tattaatgag aaaataaaaa tgtatttaaa
     5341 aggaaagtat gatagtctta aaatatcgca tgaaatatat ccaggagata aagattttga
     5401 agtagagaga caaaaggata tttataacta tgtaaaaaat aaatataata gtatagaaat
     5461 agaaatcaat gattatcatc aactatcccc cataaaaagt gttgcaatgc ttattttaat
     5521 gggagatgaa gaaaatttag aatcaagatg tagcaaatgt cctagaaaat gcttttaact
     5581 agttttcaag actaagattt gttttgtgta tgaattgcaa taattttttt ggtcatttta
     5641 tgtataaact aaaaaaatta ttgcaaagta atattctatt taatttgaac agacttacaa
     5701 actatagtag tttctttaat tcaataagtg tatttatttc ataagttggt atatgattag
     5761 tataatttat actgttattg gaatttaacc aacaagtatc tattcctgaa tttattccac
     5821 ctaagacatc agaggttaag ctattcccaa tcattaaggc agaagattta tcatgacaat
     5881 taaacttttt gagagcgtat ttaaatattt caggatttgg ttttgatatt ttcaactctt
     5941 cagatacaac cattccatca atatatcctt ttatttttga attttcaagt cttttctttt
     6001 gaactgcaga aattccattt gttattataa caatagtata ctttttttta agatattgta
     6061 atatatcgat tgcacctgga attaaaaatg tacattcacc taaaaaattt aagtacattt
     6121 tgctaagagt ttcagaatca acatccaaag atattttatt tgcaaaaagt tcaaatctaa
     6181 gtaatttaag ttcattcaaa gttataatat ttttttcaag gtcgagccaa agcttatcac
     6241 ttatatttct atatgactct atataattct caaaattaaa ttctaaatca aactcagata
     6301 gaagtttttt aaaagcatgt gattcacttt tcttaaaatc aaataaagta tcatctgcat
     6361 caaaaaatat aaatttatat gtcttcatgg tttttactcc tttacaagat gactgcaaaa
     6421 attactttac tttcactatt tgagtcattt tgccatttat gctttgttcc taaaggaact
     6481 tttacactat ctcctttgga caaagtaaat tcatcatcat ccataaaaag tttaacttct
     6541 ccctccaaaa catatgcaat ttcatctcca ttatgagtaa tcctatcaat acaagaagaa
     6601 gtatttggag ctaaatccat aattgcaaat tctaaattac cactagagtc tggaattaaa
     6661 atctcatata taacttcttt gcttccaggt aaaataactt tttttctatt atcatgtctt
     6721 actatcaagt tttttttatc tacttcagat acaaaaaatg taaataaagg aacgtttaga
     6781 gaagaggcta tagattttaa tgaatttaca gatggattag caagacctcg ttctatttgg
     6841 ctcaataatg atggtgtaac atcagctagt tttgctaaat ctcgaatact taagttttgt
     6901 ttttttcgaa cttcagctat tttttcccct aaatttaaat cattcatata aatatcctct
     6961 ctttttaatt taaaaaatat taacttaaaa taatcaagta aaatgattat gtagtatgaa
     7021 attattataa catatagaat taggttttga aaaaactatg ttcaatataa ttaaataaat
     7081 taaaatataa ttgacaaagt ggatacagaa tacttaaaat atatttaaat aaaattaagc
     7141 aaaagatagt taatttatga taggagggga cattatgcaa ataacgactt ttggagctat
     7201 atttggttta ttaatagcaa ttattttaat tataaaaaaa tttcaagctg tatatagttt
     7261 aatgttagga gcatttattg gaggattagt tggaggagca aatataacac aaactgtaga
     7321 ttttatggca aatggagcta tgaatatatc accatcaatt cttagagctt tggcatcagg
     7381 tgtattagct ggaagtttaa taaaaacagg agcagtagat aaaatatcag aacagattgt
     7441 aaaaatattt ggtgaaaaga gggctttatt ttcgattgcc atttcaacta tggtacttgc
     7501 tggagtaggc gtaaatttgg atgtatctat aataactgta gcacctatag gattatatat
     7561 aggtagaaaa ttaaattatt ctaaattgtc aatactatta gctatgttgg gagggggtaa
     7621 agcaggtaat ataatatcac caaatccaaa tactatagca gtagcagata atttttctgt
     7681 caatctctct agtgttatga tggcaaatat aattccagca attattggag ttgttattac
     7741 agttatttta gcaagtatat taataaataa aggaaataaa gtacaatcat atgaaatttt
     7801 agagcaaaga gaagatttac caagtttatt taaatcatta tgtggtccaa ttatagctat
     7861 atttctattg tttttaggaa atgtatctcc tatagttata gacccaatga tagcccttcc
     7921 aataggagga atcgtaactc taattgtcac aggaaacttg aacaattcaa gagaatattt
     7981 agcttttgga ttatctaaga tgcaaggggt atgcatatta ttacttggaa cagggactat
     8041 cgcaggaatt atacagatgt ctgaattaca acagagtaca ataggtgctt tacaattttt
     8101 aaatatgcca caatttttat tggcaccagt ttcgggtata cttatgtcat tagcaacagc
     8161 atcatcaaca gctggtgcta ctatagcatc atctactttt catgatgcta taataaatgg
     8221 tgggctatca ccgatttcag gtgcatcaat agtaaatgct gggtctagtg tattcgagca
     8281 attaccacat ggctcactat ttcatactag ttcaggtagt ataaacatgg atataggtga
     8341 aagatttaag ttaattccat atgaagcatt gataggaata gtaatgacta taatttcaac
     8401 atctattcaa ttagttttat aataaatata aatatttaat gaaaaaagta ggattttgtt
     8461 attaaatatt gaatttatta tagtaaaaaa gaagattgga gttttaaagt gaataaaaaa
     8521 aagattgtta ttatagggat tatttattca tttttagtag tattttcact tacaaatatg
     8581 tatgtaaata tggagtataa tttaaatgta tttgaatata ttaagaagtc acttccattt
     8641 acagaagaag aaaaaaagtg gttagaaaaa cacaagaatt tgatttacag ctctgaccaa
     8701 agttcacctc ctttgagata taagggtaag gaagatggac agtataaggg aattattgtt
     8761 gatttaataa attctctgtc aattcaaatt ggtagagatt tttattttaa accaaataat
     8821 tggtggaaag aatctttcgt aaattcaata gatgatagta taaagttttt tgacttgatt
     8881 ccatcaaaag aaagggcaaa taaatttata tttactgacc caatatacac tttgagtgca
     8941 aatatattga aagataaaaa gtcccaagat ataaatagtt acatggattt aaaggggaaa
     9001 acagtagcaa ttccagaagg tgattattct ataaattttt taaagcaaaa aattcaagat
     9061 ataaacattt tattgactcc agatataaag actggagtca accatttaat gtctggaaaa
     9121 gtagacgcag ttgttggtga tgaaccagta ttgcgatact atataaataa ttatggactt
     9181 tcaaataaat acagtgtttt aagtaatcct atatatacta aaaaagcagt tcttgctgta
     9241 cctaaacaat atgaagagct ggttagtatt ttaaataaag gcatttttaa acttcagaaa
     9301 aatggagtgt ataaagattt aaagaaaaaa tggtattcta cgtataatga agttgatgat
     9361 atattgtatg agcgaggaat tgtgcctagt atttatcttt ttataggtat aattttaata
     9421 tcaatttatg ttttttatag ctatacatat ttgttaaaga tagaaatcaa aaggaaaaca
     9481 gaacaggtaa tagaaaataa aaaaacttta gaggcaactt ttaatagtat tacagatata
     9541 ataatgcttg tagatgaaaa taacaatata gtagaatcga acaaagtact atatgacttt
     9601 atgggtgaaa tgagttataa gatagcagat ttgattagca tgataaaagg tgttattaaa
     9661 aataccttct ctgaaaatac taataaaact agtgaaatag aaatatacaa taaaatttta
     9721 aagataaaca cttttcctgt agagtataaa aaaaataata ctgagtatat agtagttctt
     9781 attaaagata taactaatga aaaaatagta gaggcgaaat tacttagaga aaacaaaatg
     9841 atatctatag gacaattagc ttctggggta gcacatgaga taagaaatcc tttgggtata
     9901 ataagaaata attgctatct attaaaagat aacgttacaa tggaagaagt gaatgattgt
     9961 gttaaatcaa tagaaagtaa tgtagatagg gcaagtaata taataaccaa cttgttaaat
    10021 ttcgcaagaa tatcagatga taatttagaa catataaata taagaaattt tattgaaaat
    10081 atagtgaaac ttcaatataa aatgttacag ttaaagaatg ttgaaataaa aattgactgt
    10141 gaacataatt tagtatgcta tataaatgga gaatcgttaa aacatgtatt tataaattta
    10201 atttctaatt caatagatgc aatacatcaa gatggaaaaa ttattatata ctgttatgaa
    10261 aaaaatcatt gtttatttat agattttaaa gataatggtg aaggaataaa agaagatgcc
    10321 ctaaaggaca tatttaatcc cttctataca acaaaaccaa ttggtgaggg aactggttta
    10381 ggtttatata ttacttataa tgagattaaa aagaataatg gagatataag tgttgaaagt
    10441 aaattggggg ttggaacttg ttttcatata aaaattccat taaataaaga ggtgactata
    10501 taatgaaaat actattagtt gatgatgagc tagaatatgg agtggtaatg aaaaaaattc
    10561 tacaaaagaa gggctatctt gtagatatag cactaagtgg tgaagaagct ataaatataa
    10621 ttaaaaaaga taaaaattat gatttggttt tatctgatgt tatgatgaaa aatatggatg
    10681 gagtacagct attagataga attaaatcta ttaatagaga tatagaggtt atattagtta
    10741 caggatatgg aagtattgaa aatgcagttg atgctatgaa aagaggagct ttatcttatt
    10801 ttataaaaag taatcccatt gaaaatttat tagaagaagt tgaaaaggta aaggcaagta
    10861 aaatatctgt ttctgtacaa aaaaataatt ttgaatttac tttggaatct aaaaatagag
    10921 attttaataa tatcataaaa atagcaaaaa aagctgcttg taaagatgtt aatatattaa
    10981 tcttggggga atctggtgta ggaaaagata tattagctag atatatacat agtataagtc
    11041 caagaaagaa tgagatattt gtacctgtaa attgttgttc tttttcagaa aatctattgg
    11101 agtctgagtt gtttggtcat gaaaaaggtt cttttacagg agcagtagat agtagaaaag
    11161 gtagatttga actttcgaat aaaggaactc tatttttgga tgaaatagga gatatacctc
    11221 taaatgttca ggtaaaactt ttgaggacac ttgaggacaa atctatagag aggataggaa
    11281 gtaataaatc tattaaagtt gactttaggt taatttgtgc tatgaataaa gaaccaaaag
    11341 tggaaatatc aaatggaaat ataagagaag atttttttta tagaataagt acaataacaa
    11401 taactatacc accactaaga aagagaagag aagacttaac tactcttata gaatttttct
    11461 taaataagta ccaaattgaa catgataaaa agatacactc gattgataag gaagtaaagg
    11521 attttttatt aaattacaat tatccaggaa atataagaga attaaaaaat ataataaata
    11581 gacttgtagt tttaagtgaa gaaggcaatt tatcaaaaga caatttaaat ttaatatcaa
    11641 ataatgtata catagatgat aaaataagta ttagaccttt aagagaaata agaaaagagt
    11701 ttgaatgtga gtatattgaa aaagttttat ctttgtgtgg aaataatatt tcaaatacag
    11761 ctaaaaaatt ggagattagt agaagacagt taaccaataa aatatcagag tataatatta
    11821 agtgagaaaa aaacttctca ttaagtgaga aaaaaatttc tcaaactgta ttaaaagata
    11881 aagagaaaaa atctgtttat ctttttttat tataatttta aaaattaata tttacaataa
    11941 ttattggtaa attcaatact ggtgtaaatt aaaaatgaaa atttctatga cattaataca
    12001 tagaatatat attttaaata attggcatag aaaatgcttt atatattaat aaacgtaata
    12061 aatattttta attgcgatta aatatattaa gggggatttt catatgaagc tagagctagg
    12121 gaatatattt ataaaaaatg ttgaatttgg agaaaagaca gaagtaaaag atggagtact
    12181 atatattagt agcgaagaaa tagaaaagat agtattagaa gatgaaagga ttgtaagtgt
    12241 aaatgttgaa cttgcaagac ctggcgaatc aataagaata gctccagtta aagatgtaat
    12301 agagcctaga gttaaaattg gagatgaaag caaaatattt cctggaatta taaataaagt
    12361 gaagactgta ggaagtggaa gaacccatgt acttttagga gcttgtgttg taacttgtgg
    12421 aaatatagtt ggatttcaag aaggcgtaat tgatatgagt ggtcctactg ctaaatatac
    12481 acctttttca aaaacaaata atatatgtat tgttataaaa gcaaaggatg gtatagatac
    12541 tcatgattat gaagaagcag ctagaattgc aggattaaaa ataggtgcat atgtaggtga
    12601 ggcaggaaga gaagttgagc cagatgaagt cattgtatat gagacaaagc cattattaaa
    12661 acaagtaaaa gaatatccag acctgccaac agtagcatat gttcacatgt tacaatcaca
    12721 aggactttta catgacactt attactatgg ggtggatgct aaacagatag tgcctacatt
    12781 tatgtatcca actgaaataa tggatggagc tataatatct ggaaactgtg tagcaccatg
    12841 tgataaggtt acaacattcc atcatttaaa taatccagtt atacatgact tatataaaag
    12901 acatggaaaa gatttaaact ttataggagt tattttgact aatgagaacg tattcttagt
    12961 cgataaagag agaagctctg atatggtagc taaattaata gaatttttag gagttgatgg
    13021 agttctagtg acagaagaag gttatgggaa tccagatact gacctaatga tgaattgtag
    13081 aaaatgtagt gaagttggag caaatgtagt tttgataacg gatgaatttc ctggaaaaga
    13141 tggcaagagt caatctatag cagacgctac taaggaagca gatgcagttg tttcttgtgg
    13201 tcaaggaaat ctagtggttc atttcccagc aatggaaaaa attataggaa ctttagatta
    13261 tgttgaaatg atgataggtg ggtataaagg atgcttaaat gaagacggaa gcatggatgc
    13321 agagctacaa attataatag catcaactat agcaaatggc tataatcatt tgacagcaag
    13381 atattattaa ataagtattt gaatataaag ttctgttgat gtaatcgttg gtctaattga
    13441 tgtaataact ataaagtgat ttggaggatt aagagatgag taaaatcaga gtggttcatt
    13501 atataaacaa tttctttgct ggtataggtg gagaggaaaa agcagatatt ccgccagaaa
    13561 aaagggctgg tgtagtagga cctggaatag catttcagag tcaatttaat gatgatgcag
    13621 agataatctc aactgtaatt tgtggagata cgtattttgg tgaaaatatt gagagtgcaa
    13681 ctcaagaact tttaaaaatg ataaaagaag aaagccccga tttatttatt gcaggcccag
    13741 catttaatgc aggtagatat ggagtagctt gtgggactat ttgtaaggca gttgaagaag
    13801 agttaaatat tcctgtagtt acaggaatgt ataaagaaaa tccaggtgta gatatgttta
    13861 aattagattt gcatatagtg tctacaggaa attcagcagc aagtcttaga aaggtagttc
    13921 caataatgac gaaactaggt cttaaattgg ttagaggtga ggaaatagga cctcctgaag
    13981 aggaaggata tataatgaga ggaataagaa aaaatttctt ccatgaactt agagggtctg
    14041 aaagagcaat tgatatgttg gttaaaaaga tgaaagggga aagctttgaa actgagtatc
    14101 caatgcctga atttgataga gttacaccta tgaaacctgt taaagattta tctaagataa
    14161 aacttgccct agtaacttct ggagggatag ttccaattga taatccagac aaaattgaat
    14221 cttcaagtgc tactaaatat ggaatttacg atttaacaga tatggattct atgtcaaata
    14281 aagaatttac aactatacat ggggggtatg acagagcata tgtccttgaa aatccaaatt
    14341 tagttgtacc attagatgta gttagagagc ttgaaaaaga aggtgtaatt ggagaattag
    14401 tagattattt tataactact acaggaacag gaacgagtgt tggcaattct aagagatttg
    14461 gagaagagtt ttctaagaaa ttgttagaag atgatgtaga tgctgtaatt ttaacttcta
    14521 catgaggtac ttgtactcgt tgtggagcaa cgatggtaaa agagattgag agagctggta
    14581 tagcagttgt tcatatatgt acagtagtac caatatcact tacaatagga gctaatagaa
    14641 ttgttccagc agtagggata ccatatccac ttggaaatcc tatgttagga gaagaggaaa
    14701 gtaaaaaaat tagaaagaat atagttttaa aagctttaaa tgcactaaca gaggatatag
    14761 aagaacaaac agtatttgaa taataaaaat ataatataga aataaaaaaa gatatcataa
    14821 aatagttttt gaaaattatt ttatgatatc tttttgctat ggatggaatg aagaaagcaa
    14881 ttaagtaata aaaatattta tagtaaaaca gccaattaaa gtataaagaa tgaaaaaaat
    14941 aaatagaaga ttatacataa acaaaaaaag agattttaaa atctcttttt attatatatt
    15001 aattattttt taaatttata ttttttgcat cagattttct aatagctaag tcaaaccctc
    15061 taaatctaat cactataggg tcattaagag gagctatctt tttcacttca acttcagtgc
    15121 catctattag tcctaaggct agaagtctct tcataagttt ttcatttcca gctatattgt
    15181 ctataattcc cttttgtcca agttttaagt tgtaaacagt cattattaac acctcctaat
    15241 gaaaatcatt atcaaacttt atatttataa tatataacaa aaagagtaaa ttgtcaatat
    15301 ataacaaaaa ataaacatac taagttagta tgtttataaa aaatagtata catgggatac
    15361 taatagcatc tagcaaaaaa ccacttacta atggtacaac caaaaaggct tttggagaat
    15421 caccatattt ttgtgtcaaa gaactcatat ttgccaaagc gttcggagta gctcctattc
    15481 catgtccaat caaaccactt attatgatgg cagaatcaaa atcttttcca agtactctaa
    15541 agcaaataaa tattgaaaat aggattataa agattacttg aaaagttact attaagagaa
    15601 taggaggtaa taatgtagat aattcaaata aatctatatt cataagtgat aaagttaaaa
    15661 aaattcccaa tgaaagttct tttaaaaaat ctataagttt aaaa
//
LOCUS       DAEPOZ010000076        15681 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.076, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000076 DAEPOZ010000000
VERSION     DAEPOZ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15681
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.076"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            237..1151
                     /gene="queG"
                     /locus_tag="KR440_001776"
     CDS             237..1151
                     /gene="queG"
                     /locus_tag="KR440_001776"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBH0698829.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQQKGYLTG
                     MEEPIIENRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFVDNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEMPTTHILEFKENIIDYLDYDEIQNISNKEFKR
                     RYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            1257..3494
                     /locus_tag="KR440_001777"
     CDS             1257..3494
                     /locus_tag="KR440_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0698830.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNRLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDKSDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNESSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            3691..4743
                     /gene="nth"
                     /locus_tag="KR440_001778"
     CDS             3691..4743
                     /gene="nth"
                     /locus_tag="KR440_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBH0698831.1"
                     /translation="MKNVNLKEINEKDVIVEEDINLKGMDKKDVIEKEEVNKKSIDKK
                     DIIEKEEIDKKNVKKVEVQKEIDKMISKKDEIIDEKDIENFRYIEEDKKSIKVSKKST
                     KKGETEKKTKKSSSNKATQKQSKEVKSKSKIKSESANKKDVNKILDELEKLYPDAKCE
                     LNYGTAFELLIATILSAQCTDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGL
                     YKSKSQKIKDTSEQLCELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDT
                     HVFRVSNRIGIVDEPNPQKTEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASC
                     PIKEDCNYYKELNETK"
     gene            4937..5407
                     /gene="trmL"
                     /locus_tag="KR440_001779"
     CDS             4937..5407
                     /gene="trmL"
                     /locus_tag="KR440_001779"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBH0698832.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            5443..6291
                     /locus_tag="KR440_001780"
     CDS             5443..6291
                     /locus_tag="KR440_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBH0698833.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVDEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
     gene            6681..7685
                     /locus_tag="KR440_001781"
     CDS             6681..7685
                     /locus_tag="KR440_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH0698834.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNAALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGIKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            7810..8922
                     /locus_tag="KR440_001782"
     CDS             7810..8922
                     /locus_tag="KR440_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH0698835.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQSESKSVPTSAEKDKLNSKSKNEEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDLIIAVGARFAEPIKEAATKYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            9345..10106
                     /locus_tag="KR440_001783"
     CDS             9345..10106
                     /locus_tag="KR440_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698836.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            10109..10927
                     /locus_tag="KR440_001784"
     CDS             10109..10927
                     /locus_tag="KR440_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HBH0698837.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            11107..11709
                     /locus_tag="KR440_001785"
     CDS             11107..11709
                     /locus_tag="KR440_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HBH0698838.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
     misc_binding    12270..12508
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12572..14491
                     /gene="thrS"
                     /locus_tag="KR440_001786"
     CDS             12572..14491
                     /gene="thrS"
                     /locus_tag="KR440_001786"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBH0698839.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            15259..>15681
                     /locus_tag="KR440_001787"
     CDS             15259..>15681
                     /locus_tag="KR440_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698840.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFC
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPR"
BASE COUNT         6475 a         1455 c         2899 g         4852 t
ORIGIN      
        1 gttagtaaat aaaaaagtta aataagtaaa taaaaaagat gaagtatcta aaagagttag
       61 taggtaaaaa actaaatagg taaaaagacg aagtatctaa aagagttagt aggtaaaaaa
      121 gttaaataaa taaaaaagac gaagtattta aaagagttag tagctagaaa aggttaaaaa
      181 gactaaaaaa attaaataaa tttaaaaagt caattagatt aaaaaggaat aattttatgg
      241 ataataagga attaaaagca ttttgtaatt taataggatt aaactgtgta ggagtagcag
      301 gggtagataa gtatgataat ttagaaaaga tacttaagga cagacaacaa aaaggttatt
      361 taactggtat ggaagaacca ataattgaga acagaattaa cccaagacat ataatgaagg
      421 atgctaactc tataatagtc tgtgcatttc catatcatat aaatagacaa gaaaagaata
      481 aaaattcaaa tttatcaaaa tattgtcatg gaaaggatta tcacattgta gtaaaggact
      541 ttttacaaca aatttgtgat tatatttcta aaacagtaac agattttaaa tacaagttat
      601 ttgttgataa tggaccatta gttgacaggt atttagctta tttatcagga ataggatatt
      661 ttggaataaa taataatatc ataactgatg aatatggttc ttacgtattt atagggtata
      721 tagtaaataa ttataaattt gaagaagata aaccactaga aaaaagttgt tttaaatgtg
      781 gtaagtgtgt aaaatattgt ccgggaaatg ctatattggg aaattatgat atgaacccca
      841 aaaaatgttt atcatatata actcaaaaaa aaggagattt gtcagagaaa gaaaaagttg
      901 ttttaaagaa tggcaaaaag gtatttggat gcgatatttg tcaagaagta tgtccacata
      961 atgttgaaat gccaacaact catattttgg aatttaaaga aaatataata gattatttgg
     1021 attatgatga gatacaaaat atttcaaata aagaatttaa aaggagatat ggagatagag
     1081 cttttagctg gagagggaga aatataataa agagaaatat ggaaataatt ttagataagt
     1141 atgatgacta ataataagag tctgtttcat atactttaat gtgtatttac tttaacactt
     1201 gttgtgtaaa agtcatatta tatcattaaa gttaaatatg gggaggtggg ggaatattga
     1261 aatataataa tacatctgaa ataaatccac tagattatat attgggtcaa gaaagagctg
     1321 tagaagctat ggaattaggg ttgaaaataa ataatccagc gtacaacata tatatagctg
     1381 gtgaacctgg aactggtaaa agtacttatg ccttaaaggt tttaaatgaa tatgcatcca
     1441 aaaaaaatac acataaagat tggtgttata tatataattt tgaaaaccct agagagccta
     1501 ttatagttga attagaaaag gggtttggaa gagaacttaa gaaagatatg gagaaattaa
     1561 ttgaaagtct tttagaagat tttaaaaatg cgtttgaaag tgaaaatttt gaaatagaaa
     1621 aaaatagact tctagatgaa tatgaaatag aaaaagatat gcttttgaag caaattaaga
     1681 aatatgggga agaaaaagga tttaaattaa aacagagtaa aatgggaata gtttttatcc
     1741 ctttgaaaga agaggaagat gaaagtgata agtctgatga agagttttac aaagctaaaa
     1801 gagaactaga aaatatggcg atacaggtcg tatataaaat tagaaactta gaagaaatag
     1861 ctgaaaaagc agtgttagag cttgaagaag agatagctaa acttgtagta gaacctcata
     1921 tcaagagatt gtgtgaaaaa tatgaaaatt atgataagat tcaaacttat ctggaaaata
     1981 taaagaaaga tataatagaa tatatgtatt tgttctattt agatgaagag gagttaaaag
     2041 ataagtatga taaagagcat tttattaaat acaaaattaa cctatttgta gacaatgaaa
     2101 gctcaaagaa taaagaatca gcacctgttg tagtagagat aaacccaagt ccagcaaatc
     2161 tatttggaaa ggcagagtat gattatgcaa atggaaatat aaagactgat ttcacaaaac
     2221 tattatcagg agcatttcat agggctaatg gtggttattt agtactttat gcagaccaat
     2281 tactaaaata tacaatgtct tgggaaattc ttaaaaagac tttgcagact aaaaaagtaa
     2341 tattggaaac acaaacagca attaaaccag aaaatatgcc attggatgtt aaattagtac
     2401 tgattggaag ccactatata tatgatattt tatatagata tgatgaagat tttgaaaaat
     2461 attttaaagt atttgtagac tttgatagtg aaatggataa aaatgaagat aatgaagaag
     2521 gaatagcaag atttatagca taccaatgtg ataagaataa tttaaggcat tttactaaat
     2581 cagcagttga agaggttatt aaatttagca ctagattatc tggtgattta gaaaaactat
     2641 caactaagtt taataaaata atggaagttg taatagaagg aagtgcttat gcagagttta
     2701 gaaattcgga gtatacagag cagtgtgatg ttaaaaaagc aataagtgaa aagagaaaaa
     2761 gaatcaatag agtggaaaca catatggatg aatcaattga aaatggattt actctaatag
     2821 aaacagaggg aagaaaagta ggagtgataa atggtctgtc agtcttaagt acaggagaat
     2881 actcttttgg tagagcttca aggattacag cgaccactag cccaggaagt aaaggcatag
     2941 taaatataga aagagaagta aatatgagtg gctctataca taacaaagga gtgcttatat
     3001 taggtggcta tctttctgag aattttgcac aggatttact attgtcacta aacgcatatg
     3061 tttgttttga acaaaattat ggtggaatag atggagatag tgcatcttct gcagaattat
     3121 atgtactatt atcatctctt agtggagtat caattaaaca aaatattgct gttacaggtt
     3181 ctataaatca aaagggtgat atacaagtag ttggtggtat aagtgaaaag atagaaggat
     3241 tttattctat atgtaaaact aaagggttaa atggtgagca aggcgtcata attcctagaa
     3301 aaaatcaaag aaatctcgtt ttatcagatg aagttaatga tgcagtgcga gatggaaaat
     3361 ttaagatata tactgttgaa agagtagaag aggctataga gatattgaca gatgttaaat
     3421 ttgaagaaat aaagaaattg gtaaaagaaa aattaatatc tttcagcaag atacaaactg
     3481 tgtcaaaaga ataagtttat tatagaattt aatgggtatt tatgtataat aaaaagcaag
     3541 aactttaata ggtttttgct ttttttgtaa atatatataa attaaattct tattttgtat
     3601 gttttatata ttgatgttta ttttttgttg atatgactaa actatttata aatagtgttt
     3661 tataaattgg tattttaaag ggggataatt atgaaaaatg taaatctaaa agagataaat
     3721 gaaaaagatg taattgtgga agaagatata aatctaaaag gaatggataa gaaagatgta
     3781 attgagaagg aagaagtaaa taaaaaaagt atagataaga aagatataat tgagaaagaa
     3841 gagatagata aaaaaaatgt aaagaaggtt gaggttcaaa aagaaataga taaaatgatt
     3901 agtaaaaaag atgagataat tgatgagaaa gatatagaga attttaggta tattgaagaa
     3961 gataaaaaaa gtatcaaagt aagtaaaaaa agtactaaaa aaggtgaaac ggagaaaaaa
     4021 acaaagaaaa gtagttctaa taaggctaca caaaaacaaa gcaaagaagt taaaagtaag
     4081 tctaaaatta aaagtgaatc agccaataaa aaagatgtaa ataagatttt agatgagttg
     4141 gaaaaattgt acccagatgc taaatgtgag ttaaattatg ggacagcctt tgaactttta
     4201 atagcaacta ttttatcagc acaatgtact gatgtaagag tgaataaagt tacttcagag
     4261 ttgtttaaaa agtacaacac agctagagac tttgctaatt tgagcattga agaaattagt
     4321 aaagagataa agagttgtgg tctttacaaa agcaagtctc aaaaaataaa ggatacatct
     4381 gaacagctat gtgaattata tgatggtgaa gtacctgata gcttagaaaa gcttataaaa
     4441 ttacctggtg ttggaagaaa aacggcaggg gttgtactta gtaacgcttt taatcatcca
     4501 gcaatagctg tagatactca tgtatttaga gttagcaata gaataggaat agtggacgag
     4561 ccaaatcctc aaaaaacaga atttgcactt atggaagcta taccaaaaga aagatggtca
     4621 cactcacatc atgtattaat ttttcatgga agaagaatgt gtaaagctag aaatccagaa
     4681 tgtgcaagtt gcccaataaa agaagattgt aattattata aagaacttaa tgaaacgaaa
     4741 taacttttag aaagtaagtt gtacaagagg gattttctga tataataata cttgtgaaaa
     4801 tgttagataa aaaactatta tatacatgaa ataaaaatta ttattaacat tattttatat
     4861 aatagtataa tattattgtt attctgttta taaaataaaa tttgataaat aaaagcgata
     4921 cttagggagg attaaaatgt cattaaatat agtattagta gaaccagaaa tacctcaaaa
     4981 tacaggaaat ataataagaa cctgtgcagc aacaggttca actttacatt tagtaaaacc
     5041 actgggtttt tcattggatg ataaacacct aaaaagatgt gggcttgact attgggatat
     5101 agctgatata caatattacg atagctttga agaattacaa gaaaagtatc caaatagtaa
     5161 atactttttt tctacaacaa aggctaaaca gtcacattca gatgtgaaat atgaagaaaa
     5221 ttgttttata gtatttggga aagaaacaaa gggattacca gaacctttgt tgaaagcaaa
     5281 tatggatact tgtattagag tgcctatgtt ggatgttgaa aaggctaggt cattaaattt
     5341 atcaaattca gttgctattg tagtgtacga ggctttaaga caaataggat acccaaatat
     5401 gaggtagttt caacaagcct aaatgagaaa ggagcatact taatgaaaaa tctaaagata
     5461 atctgtgaca gtttatcgga cgtgccaaaa gatttgttag ataaatatga tatagatata
     5521 gtttcattaa cagttatact ggaggaaaga gaatacaagg atagaattga tatatctgga
     5581 gatgaatttt ataaaaggct tagagaagaa aatgtatatc caaaaacttc tcaagctact
     5641 tatgcccaat ttaaggaaat ttttgagaaa tatacaaatg aggatagaga gatactttat
     5701 atagcagggt ctgccacagc tacagggacg tatcaaagtg ctgtaatggc aaagaatgat
     5761 gtggatggta aaatatatac atatgactct aacttgcttt gtatgggaac tggtttactt
     5821 gtaataaaag caggagaact tgcaagtgaa ggaaagtctg ttgatgaaat attacctgtt
     5881 ttggatgaat taaaagagaa ggcttttgtt atattttcag tagatacatt ggaatattta
     5941 caaaagggag gtagaatatc atctactaag gcagcaatag gaagtatatt aaatataaaa
     6001 ccgattcttg aaataagaga tggactagtt tcgcaaatat ctcaagtaag aggaaagaag
     6061 aatgtaattt ctaagatgat ggaattgata aaatcaaatt gtggggatga tttatcagac
     6121 caaattgtgt atataggata tagtgatgat tttaaggaaa aagaacaatt atctaaggtt
     6181 atgcaagatg aattaagacc taaagaaatt agatttttcc aaattggtgt gtgtataggt
     6241 gcgcatgctg gtcctggtgt cactggtatc atatgcttaa aaaataaata atatattttt
     6301 ggatgacatt atttattttt ttattaaatt aggatagaat attaattatt gagatgttaa
     6361 attggattta ttttggatga aattaagtgt tgatatatta gatttagttt gtagggaagt
     6421 gtgtaaatag ataagaatct attgcacact tttttatagt aagatttatt aaaaaaattt
     6481 caaaatgtac ttgtaaattg tatataaaaa aattataaat aggatgtaaa agtaaatagg
     6541 atgtaaaagt aaataggatg taaaagtaaa taggatgtaa aagtaaatag gatgtaaaag
     6601 taaataggat gtaaaagtaa ataggatgta aaagtaaata ggatgtaaaa atatcgtaaa
     6661 tgtatgagga gtgaaattta atgcgtttca aaagaatatt gatattgatg ttaactgtag
     6721 caatgatagc tggaatgttg gttgggtgtg ctaataaccc aagcaataat cctagctcta
     6781 gcaaagataa taaaagtgat ataagcgata aaagtaattc aaaaaaatct gtatcaatga
     6841 tactagatat tgaaggaaca aataatgaag ctatgaataa tgcagcttta ttagcattaa
     6901 acaatgctca aaagaaactt aatatagata caaacaaagt ggaatcagat gattcctcaa
     6961 ctttttctaa tagtatagat attttatgta atgacaatta cgatttaatt atagctgtag
     7021 gagctagatt tgcaaaacca ttggagatgg tagccaagaa atatccaaag caacaatttg
     7081 caattataga ctatgagtat gacaagcaac cttctaatat aacttcaatt tcttatgaag
     7141 ataataaatc tggataccta gctggattaa ttgctggaaa aatgactgaa agcgataagg
     7201 ttggttttat aggaggaata aagagttcta gtagggataa atttgaatca ggttttagag
     7261 agggtgttaa attttctaat agtagtataa aggatatttc agttgagtat gcagatgttt
     7321 ttaaagatag caaatcagta gaatctatag ctaaaaaaat gatggataat ggagtggata
     7381 ttattttttc tactactgaa gatgattcaa aagctgtaat agatgcagta agggcaaaaa
     7441 ataaaaaagt tatagcaacg aataaagacc aacatgagtt agctccagaa aatgttataa
     7501 gttctattgt aaaagatttt gagaatccta cttataatct gatacaaagt tttgtaaaag
     7561 gaaattataa aggcggaaaa gttatagaaa acacagttaa gtctggaagt actgggcttg
     7621 cccaaaatag ttctcaaaat attcctccag atgtgttaga atatgttaat aagaacaata
     7681 aataatctac tataatatcc aagaatactt atgactttag ttatgaggtg aattggatgt
     7741 tataatagac atatatagaa agacgtatgc agaggtaggc agacctatct aaatcaggga
     7801 gtgaattttg tgaaaataaa aaggatattg atggcttgct ttgtagtagt ttttacaatt
     7861 tttatattag ctatatgtaa ctttgatttt ggatttgata aagtaaataa gaataattct
     7921 aatatagata gtgctaaatc gaaacagagt gagtctaaaa gtgtaccaac ttcagcagaa
     7981 aaggataaat taaatagtaa aagtaagaat gaagaagatg acacagaaga tgattcatat
     8041 gtaagaaaaa ctattggagt attgttagat accgaaaact taaataacga atcatataat
     8101 aataatgtac tatcagcctt aaaaagggct gatgaagatt ttgatgttag gataaaactt
     8161 gaaaaaccaa agacttctaa aagttttaaa gagagtatag aaaaactaca taagtatgaa
     8221 ccagatttga taatagcagt tggagctaga tttgcagagc caattaaaga agctgctact
     8281 aaatatccca aacagcaatt tgcaatagtt gactatactt ataaagttca gccagctaat
     8341 gtaatttctg tatcatttca agataatgtt actggatatt tagcagggct tattgcaggt
     8401 aaaatgactg atactgggaa agtcggtttt ataggcggag ttgagggttc tagtagagat
     8461 aagtatgaat ctggatttag aaaggggctt aaaacttcaa ataaagatgc tgagttacta
     8521 gttaagtatt caaatatatt tgaaaaccct gaatcagcta gaactattgc aaaggaaatg
     8581 agagattctg atagagatgt tgtattttca gctacagaag atgatagtaa aagagctatt
     8641 caggtagcaa gagaaaatgg tggtaaagtt atagctatta ataaagacca atacgatatg
     8701 gctccagata atgtaataag ttcaattata aaagatgtag aacaacctgt ctatgaactg
     8761 ataaagaacg ttgcaaaaga tggttttaag ggaagtaaag taatggaatt taaaataaaa
     8821 gatggagaac ttggcttgac ttcaaagagc agtagaaata taccaccaga tgttttggaa
     8881 tacataaaaa aagagtataa caaagtgaaa gatacatatt aattattgat gtagaagatt
     8941 ataaaaatat actgtttaag tttagattag caataaaaag atatatttat taaatttgtg
     9001 ctaactgtac aaaatatatt gattaagttt agattggtga taaaaagata tatttattaa
     9061 atttagtcta actgtacaaa atatattgat tgagtttaga ttagcaataa aaagatatat
     9121 ttattaaatt tgtgctaact gtacggaata tactgcttaa gtttagatta gtgataaaaa
     9181 gatatattta ttaaatttga accaactgta caaagctata ctaattaagc ttagattaac
     9241 tatataaaat tataacaata agtgtgtggg cagaaaaatt ctgctacaca ctttatttat
     9301 attcatataa aaaaattatc aaaaaaaaga aaaaggagta aaaaatggaa tttacaagag
     9361 aaccatttat actagatgac gatttgtgtc tagctttggt ggacaaaaga attagttatc
     9421 taatggaaaa tgaattaaaa ttaagaaata tagagataat aaaaacaata gaatgtaaag
     9481 aattatatga aagcataaaa tatcacccgg atatatgtat ttgtaattta ggaaaaggag
     9541 atataattgt tgcgccgaat gtatataatc aatacaaaga gttattaagt agatataaat
     9601 ttaatataat taagggagaa gctaatttat taaatagata cccttatgat ataccttaca
     9661 atattgcgat agttggtgac tatgctatac acaattttaa gtatactgac aaagcaatat
     9721 tagattatat tgaaaataat aaacttaaaa agataaatat agagcagggc tattctaaat
     9781 gctcaatctg tatagtagat agaacgtcta taataacatc agataaaggt atttggaaaa
     9841 gtatgaagaa tacagaaata gagtgtttat tgatagaaaa aggtcacata aatttatttg
     9901 agatgaatta tggatttata ggtggatgta cagggttgat ttctaaggat aaactagctt
     9961 tttgtggaga tgttaaaaaa catcctgact atgaacgtat aaaaagtttt gtagaaagta
    10021 aaaacaagga gatagttaca cttagctgtg aaaatttact agatataggt tctatagttc
    10081 ctctcatgac aagaaaggag cgataacttt ggttgagaag gataatattt cttccatgaa
    10141 atttttgagg aataataatg taaacgatag attaatatat acgaatgaca ttgtagaagt
    10201 agtgatagat tctgcaaagc aagaagtgtt aaataaaaaa atacttaatg gtactaaagc
    10261 tgaacaaaga gctttaaata ttgatgagat atcaacggaa ataactagta tgataataag
    10321 tataatgaaa gagaaacttt tgaaagaata cattctaaaa tgctatgggc gtacatatcc
    10381 tgaagataaa aataatatat acatatattc actgaacata tttgctaaaa aagagatttt
    10441 tatgagagaa acagtattta ctagggtgag aaattatata gaagaaaatg attttataaa
    10501 tgtagaaggt tttattagat ttagaatgag agaatttatg aaatatattt ctgctatagg
    10561 tgatattgca gttgaggagt atttaataaa gaaagaccaa gatgaattta taagagtttt
    10621 aaaatacttt atagatactc aagaagaaaa gattgatttg cttaaagttc atataatgga
    10681 agataataca tttgttttat acgataaaaa tggaaataag atagacagta tagatgatga
    10741 agaaattata aatatggtaa taagagaaaa tttaaattat gaggactttt taataagtac
    10801 cttattaact ctatgtccca aaaaaataga aatattggat ttgttaaata ataattgctc
    10861 aaaggaaatt atagatactg tagaagcaat ttttgaaaat aaagttagta taattatgga
    10921 aaactagaat ctaagcatat aaaagctagc catataaaat taaaaaaatt ctttttaaat
    10981 cgacacaaaa gacttataat tgtatataaa gctatagtat aactagagtt attaagtata
    11041 aagaatatct acttgattaa tgcaatatag ctggcaaata tacaattgat aaggagtaat
    11101 tttataatgg ataatttaat taggatactg atattagctg ttattggtgg atttataggt
    11161 tatgttacga atgtagttgc tataagactg atatttagac caatagagcc tattaaaata
    11221 cctattttaa atatagaaat agtaggtctt ataccaaaga gaagagcaga aatagcagca
    11281 aatgtaggag aaataattca agaagaattt ctttctatgg acgaaatact tgcaaatatc
    11341 attactgacg aagataagga agaagttgta agatacataa aagcaagggt aaaaattata
    11401 atacatgaga aagtttcatt tatcccgagt ggtataaaaa acatgataca agattatcta
    11461 ggagagataa ttgagtctga agttaagcaa agtatagatg aattgagtaa aaatataata
    11521 aataaagcaa atgaacgtat agatatacaa aaaatggtcg aagataagat taatgaacta
    11581 gatttatatg aattggaaga gataataata agaatagcta agaaagaatt aaaacatata
    11641 gagtttttgg ggcttgtctt agggttttta ataggtatag ctcaagggtt aattactatg
    11701 tttatttaaa tatatgagaa gtagcattta tttaagtact aattatattt acctatttga
    11761 gtgaattact tattaatagt atttagatat taattgtatt tacctatttg agtgaattac
    11821 ttattaatag tatttagata ttaattgtat ttacctattt gagtgaatta cttattaata
    11881 gcatttagat attaattgta tttatttatt tgattgtgtt acttattaat agtatttaag
    11941 tatttactgt actacttatt aactatatta agtattgatt gtatttaagt atacaaaacc
    12001 taaaaagagt acaaaaatta gaagttagtt ttaataaaaa ttagttttag tatttatcta
    12061 gtcatatcta tcatgcatgt ggatacgttt taaaaatttt aaatttatga aaatattata
    12121 atatcactat aaatcaagag attaatttaa tataaaaata taagaatatt ggtttacagc
    12181 tgattgagat gaaaaataat gggtaattaa tatattacat taatgttgat tgatgtataa
    12241 ctatctgtta taatgaaaat atattaaaaa tcatagtgtt gaagaggaag agtagagtag
    12301 aaaccattct cagagaattt gggtctatgg ctgagagccc atgtaatagg tctatttgaa
    12361 aactaccttg gagctgagct tattagcagt atttgtattt gtactatacg tgaaagtgct
    12421 cgggtgacga ccttatagtc atcaagaggg ttgtatatac ttgtatacgc caacttgggt
    12481 ggaaccgcga attaatatcg tcccaaacat atttggggcg attttttatt acaaaaaatc
    12541 taaaaattat ttcaataagg aggaaaaaac aatgattaaa gttgctttaa aagatggctc
    12601 aataaaagaa tttgaaaatg ctatttctgt tatggatgta gctaaatcca ttagtgaagg
    12661 attagctaga aatgtggtag cagcgtctgt gaacggagag gttgtaggtc tggaccacat
    12721 tatagatact gattgtgact tgaatttatt taagtttgaa gataaagaag gtaaggaagt
    12781 ttttagacat acatcagcac atatattagc tcaagctata aaaagacttt acccagaagc
    12841 aaagcttgct ataggaccaa gtatagaaaa tggattctac tatgatatag atttagacca
    12901 tagattagtt ccagaggacc tagagaaaat agaagctgaa atgaaaaaaa tagctaaaga
    12961 agatttaaaa atagaaagat ttgaacttcc tagaaatgaa gctttagagc ttatgaaaga
    13021 acaaggagaa gactataagg tagaacttat atctgattta cctgaaagtg aaataatatc
    13081 tttctacaag caaggtgatt ttactgattt atgtagagga ccacatttac catctactaa
    13141 aaaggtaaaa gctgttaaat tacaaagtgt agcaggtgca tattggcgtg gtgacgaaaa
    13201 taataagatg cttcaaagaa tatatggaac ttcttttgag aaaaacaaag acttagaaga
    13261 gtacttacat ttactagaag aagctaaaaa gagagaccac agaaaattag gtaaagaatt
    13321 aggattattt atgatacctg aagaaggtcc aggattccct atgtttttac caaaaggtat
    13381 ggaacttaaa aatgaattat taaaattctg gagagaaata cacagaaaag ctggatatat
    13441 tgagatagaa tctccaatca tattaaatag aaaattatgg gagacttcag gacactggta
    13501 tcattacaaa gaaaatatgt atacagttaa aatagatgat gaggattatg ctataaaacc
    13561 tatgaactgt cctggtggat taatttacta taactctcaa ctacattcat atagagattt
    13621 cccaatgaga gttgcagagc ttggtagagt tcatagacat gagttatctg gtgcattaca
    13681 aggattaatg agagttagag catttactca agacgattca catatattca tgttaccaga
    13741 gcaaattaag gacgaaataa aaggagtagc taacttaata gatggtatct acaaaacttt
    13801 tggatttgaa tataatttag aattatctac aagaccagag aactctatgg gaagcgacga
    13861 agagtgggaa gcagcagaaa atggattaag agaagcttta gaagagttag gtctaccata
    13921 tactattaat gagggtgatg gagcattcta tggtcctaag atagacttcc atctaaaaga
    13981 ttgtttagga agaacttggc aatgtggtac tatccaatta gatatgcaac ttccaagaca
    14041 atttgataat acttatattg gtcaagatgg tgaaaagcat agaccagtta tgattcatag
    14101 ggttgctttt ggtagtatag aaagatttat aggtatatta atagagcatt atgctggtaa
    14161 attcccagtt tggttatctc caactcaagt taagatactt cctatatctg ataaatttat
    14221 ggattatgca aatgaagtta agaaagagtt atttgataag ggtataagag ttgaattaga
    14281 tgatagagct gaaaaaattg gatttaagat aagagaagct cagcttgaaa aagtgccata
    14341 catgttaata gtaggtgaaa aagaagtagc tgataataat gtttctgtta gatcaagaga
    14401 taaaggtgaa ataggtagta ttaaattaga tgaatttata gctagtattt caaaagaaat
    14461 tgaatcaaga gaaagtatta ttcaagacta atatataata tattagtagc taagatattg
    14521 ttatataaga taagtttaat caatatgttg atatgttgat acatagtgat taaatttatg
    14581 aaaagatgag tataattaga ggtacagtta atcatatttt tatgattaat gtacctctag
    14641 ttttttgtat ggtagattta ttaataaata tatcatagaa gtgttttgaa catggaaata
    14701 tttatgtgca ttctttaaat aaaagtttat tttttgaatg ataattaagt gcttgttggt
    14761 aaggttatgg ctgtaaaaat cataacttta tttttatatt tgttccattt tcataaaaat
    14821 tattatccct ttaagaaaat ttcttaatcc tcttgaaata tttccttttt agtctaaagt
    14881 taaatttctt tcttcaaaat gaatatttaa atgaactaaa atttaagtac attttctaat
    14941 cttatgagtc tatttttcat agataattgc cctataatag aagaatctat gttggggcta
    15001 tgtggtgaca ataacagcat tgtcagtgat taattaatgc tttataaatg attaattagt
    15061 gttttatgtt atttttggac ttaccctcta tacatagatt aatttattta actttagata
    15121 tagagagaat gtcaagtaca tacacagaca ttagagttta agaaagtgtt tatggagcaa
    15181 gaacttttaa aagaagtagt gacttagttt aatttcaaga aatgataaat acaaaagagg
    15241 aaaaagagag gtgttgaaat gatagaaaaa gtagaagaaa aatctaagat tattaaaaca
    15301 tcaaaagaat ataagcgaac tataattgaa gaaaaagata aagaatggac caaaaaatta
    15361 aatgatatac ttagtagaag tagtttttgt aatgatgaat tagataaaat caaggcgtta
    15421 attccaaaag agattttgac taatgaaaat gttggagaag ttgtgactga agatggatat
    15481 gctaagcccg agtatgatga ggtatttaaa tcattgttca ctttaaataa tgaatatgat
    15541 ttacttgtaa attttataaa tgatattcta aaagatgctc catatgctaa caggaatatc
    15601 aaaccgttca ctcaaataaa gagaataatt agagttgaaa ctgatccaac aattaattac
    15661 attggagaga aaagacctag a
//
LOCUS       DAEPOZ010000077        15675 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.077, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000077 DAEPOZ010000000
VERSION     DAEPOZ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15675
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.077"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            55..1314
                     /locus_tag="KR440_001788"
     CDS             55..1314
                     /locus_tag="KR440_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBH0698841.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVEEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            1375..3162
                     /gene="iorA"
                     /locus_tag="KR440_001789"
     CDS             1375..3162
                     /gene="iorA"
                     /locus_tag="KR440_001789"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HBH0698842.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEMNDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            3164..3739
                     /locus_tag="KR440_001790"
     CDS             3164..3739
                     /locus_tag="KR440_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HBH0698843.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            3792..4862
                     /gene="buk"
                     /locus_tag="KR440_001791"
     CDS             3792..4862
                     /gene="buk"
                     /locus_tag="KR440_001791"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBH0698844.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            5080..5781
                     /locus_tag="KR440_001792"
     CDS             5080..5781
                     /locus_tag="KR440_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698845.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKRSN
                     EHKCKSSIVIKNEQN"
     gene            5840..6346
                     /locus_tag="KR440_001793"
     CDS             5840..6346
                     /locus_tag="KR440_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBH0698846.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEMYELLYE
                     IDNNKLNI"
     gene            6521..7543
                     /locus_tag="KR440_001794"
     CDS             6521..7543
                     /locus_tag="KR440_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBH0698847.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(7690..7998)
                     /locus_tag="KR440_001795"
     CDS             complement(7690..7998)
                     /locus_tag="KR440_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBH0698848.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(8058..8486)
                     /locus_tag="KR440_001796"
     CDS             complement(8058..8486)
                     /locus_tag="KR440_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698849.1"
                     /translation="MNNKLHELEKNLPETSCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            complement(8709..10166)
                     /locus_tag="KR440_001797"
     CDS             complement(8709..10166)
                     /locus_tag="KR440_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBH0698850.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            10584..11498
                     /gene="nadA"
                     /locus_tag="KR440_001798"
     CDS             10584..11498
                     /gene="nadA"
                     /locus_tag="KR440_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBH0698851.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKLLSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            11511..12812
                     /locus_tag="KR440_001799"
     CDS             11511..12812
                     /locus_tag="KR440_001799"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HBH0698852.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            12793..13629
                     /gene="nadC"
                     /locus_tag="KR440_001800"
     CDS             12793..13629
                     /gene="nadC"
                     /locus_tag="KR440_001800"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBH0698853.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
     gene            13885..14217
                     /locus_tag="KR440_001801"
     CDS             13885..14217
                     /locus_tag="KR440_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBH0698854.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            14379..14564
                     /locus_tag="KR440_001802"
     CDS             14379..14564
                     /locus_tag="KR440_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698855.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            14591..>15675
                     /locus_tag="KR440_001803"
     CDS             14591..>15675
                     /locus_tag="KR440_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBH0698856.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLYNDSEEEVIEL
                     EEEKRSFMENMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSTIPKMKEVIGNHA"
BASE COUNT         5981 a         1741 c         2847 g         5106 t
ORIGIN      
        1 tagtaattgc ttaatatata atgtaagagt aaatacaatt ttaggggtga tggtatgagt
       61 caaagtatgg ttggtaagca tgcaatgtgg ccaaaagaaa atgatgttat atttagcata
      121 tctggtagag ctcaagcagc agagaaggct tttggtatgg ataatgtaat aaatgcaaca
      181 ataggcgctc taatggatga ttcaggaaaa ttaataacaa tgaaaactgt atatgaagaa
      241 tataaagcat tagataactg tgagataggt gcatatgcag ctcttgaagg tcaaccagac
      301 tacttagaag cagttaaaaa agtatttttc agagattact taccagaagg acatataaga
      361 gtattagctt caccaggagg aagtggtgca ataaaattag ctgtatggaa ctatactaat
      421 gaaggagatg aggtattaac atctgattgg ttttggagtc catatgtaag tatagctgaa
      481 gaggctaata gaaaagtagt taattaccaa ctttttgatg aaaatagaag gtttaatttt
      541 gagtcgttca aagaaaaatt tgtaaatata gcagaaaaac aaggtagagt ctttacaatt
      601 ataaacacac cagcgcataa tccaactgga tatagtgttg cagatgatga gtgggataaa
      661 atattagact tatcaaaaga agtcgcaaaa gataaagaca aaaagataat atttttcgta
      721 gattcagcat atattgactt tgcaggtgat gatgatgtat gtagaaagtt ttttaagaaa
      781 ttttctaatt tgcctgaaaa cgttctcgta ttggttggat tcagtatgtc aaaaggattt
      841 acagcttatg gtatgagaat gggggctata atttgtatat cttcaagtga agatgtagct
      901 gaagagttcc attactcatg tgttcattca tgtagagcaa attggtcaaa ttgtaataga
      961 agtgccatgg cagtattatc taacattgta aatgacccta aaaaattcaa agaatatgag
     1021 gatgaaaaag aaatatacaa aaacatgctt actagaagag cagatgtatt tgtagaagaa
     1081 gctgaaagag taggattaga aattcttcca tatatagcag gtttctttgt aagtatacca
     1141 tgtgataatc caaaagaagt atgtgaagaa ttgactaaac ataatttatt tgcagttcca
     1201 ttaaaaatgg gcttaagatt tgcagtttgt gcagtttctg aagataaatg taagaaagca
     1261 ccaagtatta taaaagaagc acttgaaagt ttagaagtaa aaataaataa ttaatttcat
     1321 tgaacaaatt gggtggacta tgaatttaat aattgtagat ggagggaatt ttatatgaaa
     1381 caattgatga caggtaatga agcaattgca cgtggagcct atgaggctgg tgttaaatat
     1441 gcatctgcat atccagggac tccaagtaca gaaatattag aaaatatagc aacatataaa
     1501 gatgcaatag ttgcagagtg ggctccaaac gaaaaggtag cacttgaggc tgctataggg
     1561 ggttcaattg caggagcaag aacaatggct tctatgaaac atgttggttt gaatgttgca
     1621 gctgatccaa tatttactta tgcatataca ggggtaaatg gtggtatggt acttattaca
     1681 gcagatgagc cgggaatgca ttcatctcaa aatgagcaag ataataggat gtatgcaaaa
     1741 tttgcaaaga tacctttatt tgaaccatca actagtcaag aagctaaaga tatgataaaa
     1801 gaagcttttg aagttagtga aaaatacgat acaccagttt tatatagagt tacaacaaga
     1861 ctttgtcatt caaaaggatt agtggagtgc tatgatagag aagaagttga aattaaagag
     1921 tatgttaaaa atgctaagaa aatggtaact gttccagcaa atgctcaaat aagaaggggc
     1981 gttgttgaag aaagaatgga aatccttaaa aaattctcaa atgaaactga tttaaactac
     2041 tatgaaatga atgatacaaa aataggtgtt atagcttctg gtatgtgttg taattttgcc
     2101 aaagaagttt ttggaaaaaa tgcatcatat atgaaactag gatttacaaa tccattacct
     2161 tatgaaaaaa taaaagaatt tgcagaaaaa gttgataaaa tatatgttat agaagaaaat
     2221 gacccattta tagaagaaca aataaaagca tatggaatag attgtattgg aaaagatgta
     2281 ataccaccat atggagaaat gacaccagat gttttaagaa aagctatatt tggaaaaaca
     2341 aatgatacta tagaatacaa atctgaatta gtaacaccaa gaccaccaag tttctgtgca
     2401 ggatgtccac atagaggatt cttctatgaa cttggaaaga gaaaaaattt aatagttggt
     2461 ggagatatag gttgttatac tcttggattt gctcctccat ataatggaat agattatgtt
     2521 gtttgtatgg gttctgcttt tggaactgca catggagctc aaaaagttct taatatgaag
     2581 gatgataatg aaaaaagatt ggtaggtgta cttggagact caacattctt ccatacagga
     2641 ataaatggac ttttagatgt tgtttacaat agaggtaatt ctatttcggt tatattagat
     2701 aatagaataa ctggaatgac aggtcaccaa gaaaacccag gttcagggta tactttacaa
     2761 ggtgctaaaa ctaaagaagt tgatatagaa ggattagtta aagcttgtgg aatagaacat
     2821 gtaagagtta taaaccctaa caatttaaaa gaagtaaatg aagcactaga ttgggcttta
     2881 gctatagaag atgaaccatc agtaataata acaagatggc catgtgtact taaaaaattc
     2941 tctaaagaag atatagaaga atttaataat ccatttaaga ctaagtgtaa agttgaccat
     3001 gataaatgta taggatgtaa attatgtttg aaaactggat gtccagctct atcatttgat
     3061 aaagagaata agttatcaaa tatagataga aatcagtgtg taggatgtgg agtatgtgca
     3121 caagtttgtc caaaacaagc aatagttaag gaggaaaaat aatatgacta agagtatact
     3181 tttggtaggt gtaggaggtc aaggaactat acttgctagt aaattattaa ctatgggatt
     3241 gatggaagct ggatatgatg taaagatgag tgaaatacat ggaatgagtc aaagaggagg
     3301 ttcagtttcc tcacaagtta gatatggaga ttgtgttcat tctccagtta tagaaattgg
     3361 tggagcagat atattagtat cttttgaaaa aatggaagct ttaagatggt tcaattattt
     3421 aaaaccagaa ggaaaagctt tagtaaataa tcataggata gattctatga ctgttttaat
     3481 cggaggagct gaatatcaag aaagtgatat agatgcagag ttaagtagat taaatgcaaa
     3541 agttataaat gcagctgata aagctgaaga actaggaaat tcaaaaataa tgaatgttat
     3601 tttattagga tgtctagtta aatctatgca attagagtct attgattggg aaaaaattat
     3661 aagtgaaaat gttaaaccaa aatttgttga attaaacata aaagctttcc atgaaggtat
     3721 ggaaatggtt agcaaatagt ttataataga tataaataga ctatagaaat taaggtgaaa
     3781 ggaagaaaat tatgacttac agaatattag ccataaatcc aggttctact tctacaaaaa
     3841 tagcagtata tgatggagaa gaacaaattc ttgtgaagac gatagaccat ccagctgaag
     3901 agattgcaaa atataatact atacaagacc agtttgaaat gcgtaaggaa gcagttttga
     3961 atattcttaa agaaaatagt atagacttaa aatctcttag tgcaatagta ggaagaggtg
     4021 gagttttacc accagtaaaa tcaggagcat atctagtaaa tgaagaaatg attgatgtac
     4081 taagacatag accagtactt gaacacgctt ccaatttagg tgctgttgtg gcacatgcaa
     4141 tatcagaacc tcttggaatc aactcatata tttatgattc tgttgcagta gatgagctta
     4201 tagatgtagc gagaatatct ggactttgtg gaatggatag atcaagtgca gggcatgcat
     4261 taaatactag agcaatggct ttaaaatatg ctaaggataa aggaaaagat tataagagct
     4321 taaacttaat agtagctcac attggtggag gagtaagtat ttatcttcat gaaaaaggaa
     4381 gaatggttga tatgctatct gatgatgaag gaccattttc tccagaaagg tcaggaagag
     4441 tacctgctac aaaattagtg gctgcctgtt attcaggtca atattcagaa agagaaatga
     4501 ctaaaaagat aagaggtaaa ggtggtatag tttcatacct aaatactgta gatgctagag
     4561 aagttgaaaa aatgatagca gaaggaaatg aagaagcaaa aattatttat gaagcaatgg
     4621 cttatcagtt agcaaaaggt attggagagt tagcaactgt agtagatgga aaggtagatg
     4681 ctataattat aacaggtgga attgcatatt ctgaaatgtt tacttcaatg gttaaaaaga
     4741 aagttgagtt tatagcacca gtagaaatta tggcaggaga aaatgagttg gaatcacttg
     4801 cttttggaac tttaagagta ctaaatggag aagaagaagc tagaatttat agtgaaaatt
     4861 aataagaaaa attaaaaatg tttatatact aaaaaagtgg tatatccgtt ttttatacac
     4921 gaaagcttag agtataggtg aaaatgtata aaatttggat tgccactttt tataattaaa
     4981 attttatctt aatctaataa ataattctgt aaaatttcgt atttatatat atattttatt
     5041 ggtatttatt gttattatta ttatgaggag tgatttttga tgggaaaaat aagtttaaaa
     5101 agtatgataa gattattttc aggttttttt atatttgcaa tttcttctgt gcttatgatt
     5161 aatgcacatg taggtcttat gccttgggat gtacttcacc aaggtttatc tataaagcta
     5221 ggcataacta taggtcaggc ttctataatg gtaggagttg taattgttat attggatgca
     5281 gtttttggtg aaaatatagg atggggtact ttactcaaca tgacatttat aggaattttt
     5341 attgatttag taatattttc tggagtgata cctcatgcaa gcaacactta tataggtgtg
     5401 tttatggttg ttataggaat tattttggca gctatagcaa gttttctata cttaggtgtt
     5461 tgtttaggaa gtggaccgag agatgggctt atgattgctc ttcagaaaaa aactaataaa
     5521 tctgtacgtc ttgttagaac aattcttgaa atactagcat tagttgttgg gtggttatta
     5581 ggaggttctg taggtattgg tacgcttgta agtgctttag gtttaggata tgtattacaa
     5641 atagtgttta gaatatttaa atttgacaca aagttgctga aacatagatt tattatagat
     5701 gatataagag aatggaagga aaagagaagc aatgaacata aatgtaaatc aagtattgta
     5761 ataaaaaatg agcagaatta attatgctaa aatatatttg tagtttatct aatttatagt
     5821 aaagtcagga gattttgaaa tggaattatg ggatttatat aatgctgatg gaattaaaac
     5881 aggaaatgtt ataaaaagag gtaattctat tgaagaaggt tattaccatt tggcagttga
     5941 agtgtggatt ttaaatagta actctcaaat tttaattcag aaaaggtcta aaagtaaaaa
     6001 gacattgcca aatatgtggg gaatgacaac tggatgtatt gtttctgggg aagatagttt
     6061 agaaggagca attcgcgaag caaaggaaga aatagggata gatataacta aagatgagat
     6121 gaaagttttt agaagtatga ttcatgaaga tactctttgg gatgtatact tagttaaaaa
     6181 agaatatgat atatccaaag ctattttaca ggaggaagaa gtaagtgata taaagtgggt
     6241 aagcacagat gaaataagac aattactaaa agagggtttg ttttttgaat atccagagat
     6301 gtacgaattg ctatatgaga tagataataa taaattaaat atatgatgta gagcagattt
     6361 cagtataagt aagattattt aaaatgtatc ttctctaaat tttaatattg ttttatgaaa
     6421 attaataagt tatttttagt atttttctac ttgttttttg agattaaaat aaaaagggca
     6481 gtcattataa agtcataaaa atttggggag gatttaaact atggaagata acaaaggatt
     6541 ggcacgtgat agttcaaaat ataaaattgt ttctatatct gtcgtagtaa ttattctaat
     6601 tacattttgg tatatgttaa atatggtgct tttaacattt attatgactt ttattttcta
     6661 taacttgctt gtagccacaa gaaaacgaat taagaagttt tcttctttaa atattccaga
     6721 ttcattgata attatagtgt tatatgcact atttgctata ttgttagtat tgattagcta
     6781 tgcagttgta ccaataatta tagttcaact tacagagctg agtagggttt ttagtgattt
     6841 tgatgtaaat caatttgctc aatctttagg accaaaattg tatcctattg tctcaaagct
     6901 ggattttaat aaatatattt cacaagcagg gctacttata gcatcaacag caacaaaagt
     6961 tggaagtttt ggagtaaata tattattggc atttttactg agtttactcc ttttacttga
     7021 aaaaaatgaa attaaaaatt ttggagataa actttctgat agtaagattt cttttattta
     7081 taattcgctt gtattttttg gaaagagctt tgttaaaaat tttggtgaag taatgaaagt
     7141 acaagttatg attgccttta taaattctgt tgtttctatg attttcttag gtttcatggg
     7201 attccctcaa atatgggctt taggatttat gatttttgtg ttgggtctta ttccagtagc
     7261 aggagttata gtgtctttga ttccattaac tgtaatagca tttaatacag gaggaataac
     7321 aaaggttttt ggagttttac ttatgatttg tatagtgcat gcagtagaaa catacatatt
     7381 aaaccctaag ttgatgtcga atagaactaa acttccagtt tgttttgttt ttattatttt
     7441 acttgtagga gaacattatt taggtgtatg gggattacta attggagttc caatatttat
     7501 gttccttatg gatattctgg gtgttaaatt tacttctagg taatatatta aaataataat
     7561 ttatttttat atataaatta tgaattgttt taaaaaagaa cctataaata gacatcatta
     7621 ataaagacat ctatttgata ggttcaattt tgctttgtat aaatactata gaattaaata
     7681 aaaagaaatt tatttcattt caaaagtttc acaacttgca tttctttcat ttatatgaac
     7741 tttttcagct ttgcaaagtt catttttatt gtatttacat tttacagctt cacaatgaat
     7801 gttatctgta tcaacttggt tagtttcatt agaacgacta gttataccag atgtatattt
     7861 gtcttcaaaa gaagcacaat aagtattttc tgtagtagtt gcttgcattc catctacttt
     7921 tatactacca gcataacaat atccactagt attataagca caattagtag cagcacaatt
     7981 taaattatta tttttcataa ataacctcct aagttttatt taaacttatt atgtgaagtt
     8041 atctaaattt tatacactta tttacaatta catttatcac atttaacaac tacatctttg
     8101 gaagtttccc ttcctaaatt tttattaaca tgatacacat caacattttc agttgatcca
     8161 catatagaac atttaaaatt gttttctagt agggttcttt ctttaaataa cttccaatac
     8221 atagaattga tgtaatcatt ataatttacc ttgaggcatg tctcaagaaa gtttatgtat
     8281 agattgtcta aatctcctgt atttaattct gtatattctg gttcaaaata ttcacaacat
     8341 cctctaaaat caggctttgt atctgaaata acacatctaa tatcatatct atagtctagg
     8401 ctaggcccct taaatgaaag atgtgtgcaa aaagaacaag atgtttctgg tagatttttt
     8461 tcaagttcat gtaatttatt attcataagt attcctccaa aaaatttatt ttaagtatat
     8521 aaatgaatag attattttaa aacttttatt tatttatatt agagatttag gataaatatt
     8581 ccaataagga gtaaatactt aagtatttat tactgtatta aataaaaaag aggcaaatct
     8641 aacttgagat tgcctacttt tttatcattt tttaataaaa tttattctaa gtattatttt
     8701 atttagattt agtcagccaa tttagtatcc gctttgcttt ttaactttct accagagcgt
     8761 acagttaaaa tagcatataa tacagtaaat atggcagcta aaacatatga tatatttatg
     8821 tttaagttaa atccaatttg agcattaaat atataactga atataacaaa agaataaaac
     8881 ataccaggaa ttaagcttat aatataattc ttttctttta ttataaggta aactgtaatc
     8941 attgcaaatg caaatatggc aatagtttga ttactccaag agaagtatct ccacaatata
     9001 ttaaatccag aagcactaag ctttgcaaat ataagaatgg ctatgacagg aataaatata
     9061 cagattgaca atataacacg atgtttcttt tccttttggt cataatgaaa gtaatctgca
     9121 agcattaatc ttaaagatct taaagctgta tctccagaag ttattggtaa aacaattaca
     9181 cctattatag ctatgatacc accaatagaa ccaagtaaat ctcttgcaac caaacctatt
     9241 acatcaggag aacctactaa ttccttaggt attcctttat tgtaaatacc catagcagct
     9301 gcagcccaaa tcattgcaat taaaccttct aaaatcatca tattataaaa agtagttttt
     9361 ccttcttttt catttgaaac tgatcttgct ataagagtag cttgagttga gtggaatcca
     9421 gaaacgatac cacaagcaac tgtaacaaag aatgtaggaa taagaggaat accatcagga
     9481 tgaatacctt tccaatttgc aagacttaaa ttagctaaat cataaccttg tgtaaagatt
     9541 ccaattccaa caccagcagc agataaaagt agaatagctc caaatattgg atataccttt
     9601 cctatgattt tatcaattgg aaagagtgtt gcacacaagt aatatagaaa gataagtcca
     9661 taaactatcc agacaactgg attatttata gtcgatttca tatttaaaat ttgagtaaca
     9721 actaaatccc caggtgtata aataaaaact gcaccaacta aaagcattaa aagacaaagg
     9781 aatatattat agacttgaaa tactttatta cctaaaaatc tactgataag agaaggcatt
     9841 tgtgctcctt tttctcttat actaatcatt ccactcatat agtcatgcaa agctcctcca
     9901 aatacacagc caataggtat tagtatgaat gctattggac caaacaatat accttggata
     9961 ggtcctagaa ttggtcctgt accagctata ttaagcaact caattagact atttttccat
    10021 tttttcattg ggacatagtc tactccatca ctttgagcaa gtgcaggtgt ttttctgtca
    10081 tctgggccaa atactttttc acaataagca ccgtataaag cacctcctat tagtaatata
    10141 gccaagccga ttaagaatgt tgtcataata tcaccccatt tataaaattt tttggtcttt
    10201 gtatacatat tatctaaatg tgtagattta tattgaattt aattctggac aacctacaaa
    10261 ttctataaag tattacaact aatataattt cagaaataaa tacatataca taaccatgta
    10321 cttagtatac aggtatttgg taatattttc aatgttatag caataaagaa tataaacaaa
    10381 attctgataa tattttacaa aaaaattaaa aaatataaaa attagtttaa atagatggag
    10441 aaaaaaggaa aatcaattga gaatataaat gtctatatct taacaatatt aagaggttta
    10501 aaattcttga agaaagaata gtcaagtgat ataatgggtt tacaggtgta tatacactag
    10561 tacatataga aagcggtgca attatggata aagatttaac atatcaaatc aaagagctaa
    10621 agaaagaaaa aaatgcaata atacttgccc atttttatca accaccagaa atacaagaat
    10681 tagcagatgc agttggtgac tcatattatt taagtgagat tgctagagat tgtaaagaag
    10741 aagtggttgt attttgtgga gtaaggttta tgggagaaag tgctaagcta ttatcacctg
    10801 aaaaaactgt attgatgcca gtaagtaacg ctgggtgtgc tatggcagat atggtagatg
    10861 aagaaggcgt gataaaatta aaacaacagt atccaaatgc tttggttgta tgttatataa
    10921 actctacagc taaagttaag gcacattgtg atgtttctgt tacatcttct agtgcaatta
    10981 aaatactaga aaacatagat aataaagaaa ttatattcct tccagataaa aatttaggag
    11041 gatatattgc agaacaattt ccagataaaa actttatatt ttgggacgga tattgtaaat
    11101 accataacaa tataagagct gaagaaataa ttgaattaaa agataagtat aaaaatgcag
    11161 aagtattagt acatccagaa tgtaaaaaag aaataagaga tttaggagat tatgtaggta
    11221 gtacatccgg aataataaaa tatgctacta atagtaaaaa caaagacttt ataatagcaa
    11281 ctgaagaagg tatattacat gaattaaaga aaaataatcc taataaaaac ttttatattc
    11341 ctggtggaaa aatactatgt acagatatga aaaagacaac attagaaaat ttatatagta
    11401 cacttaaaaa tatggaaaat gaagttattg tagaagatga aatcatggaa aaggctttaa
    11461 attcactgct taacatgcac aaattagcag aaggatagag ggatagactt atgaatttag
    11521 aacaagatgt tttaatagtt ggaagtggag tttcaggatt atactgtgca ttaaatttag
    11581 ataaaagttt aaatgtgcta gtagtatcga aatcaacaat tgaaaacaac aatacatatc
    11641 ttgcacaagg tggaatttca actgctagaa atattgatga tatagaatcc tttgtagaag
    11701 atacaatgaa ggctgggcag tataaaaata gagttgaagc agttcaagta cttgcagatg
    11761 aatctataga aaatgtaggg cagatagtag aatatggtat gcctttagac aaagaaaatg
    11821 gagagataga ttacacaaga gaaggagcac atagtgtaaa tcgaatagtt catagtaagg
    11881 ataatactgg agaagttgtt tttaaaactt tattaaaaga agctaaaact agagaaaata
    11941 taactcttat agaagatgca tatcttttag atatattaaa agatggcaat aaatgtatag
    12001 gagctagaat ttttaaatct aaaaaagaga ttcatgtttt ttcaaaaata gtagttttag
    12061 ctacaggtgg aattggtggg ctttttaaaa attctactaa tcaaagacat cttacaggtg
    12121 atggaattgc aatagcttta agaaataata taaaaataga aaatttagaa tatattcaaa
    12181 tacatcctac agccttttat gaagaaaata atgaaggtag gagaatgctt atatctgaat
    12241 cattaagagg agagggagct aaacttttaa ataaaaataa agaaagattt gtggatgaac
    12301 ttcttcctag agatgtagtt tcaaaagcta tttttgagca aatggaaaaa gataaattac
    12361 cttatgtgta tttagatgca acacatttag atagtgaata tttaattaat agattttctt
    12421 ttatttataa tgaatgttta gctagaggaa cagatataac gaaagaatgt ataaaggtat
    12481 ctcctgcaca acattatttt atgggcggga tacatgtaga tttggattca aaaacatcta
    12541 tggaaaattt atatgcagtt ggtgaaatta gttgtacagg tgttcatgga gccaatagat
    12601 tggcaagtaa ttcattactt gaagggcttg tattttcaaa aagagcagct aaaaatataa
    12661 actcagttat agataatgta aaagttaagt ttatagatgt accagatatg gatatagata
    12721 tagaacaggt aaaaaaagaa aataaaatat tagtcataaa agaaattgaa agaacaagtg
    12781 aggattttgg cgatgaatta tttgattatt gatagaatga tacaggatgc tttgatagaa
    12841 gatgttccaa gtgaagatat aacaactaat tctattgtgg atgaaaattc taaatctact
    12901 gtagatttaa tttgcaaaca agatggtata atagctggct taggtgtatt taagagagtt
    12961 tttgagatac ttggtgatgt agatgtaaaa ttatataaaa atgatggtga tagagtaaag
    13021 aatcgtgaaa agatagctgt attaacaggt agtactagaa acttacttgt aggagagcgt
    13081 gtagcattga attatttaca gagaatgagt ggtatagcta ctattacaag taggtatgtt
    13141 gaaaagcttg aaggtacaaa tacaaaatta ttagatagta gaaaaacgat acctaatctt
    13201 agaatattag ataagtattc tgttaaagta ggtggaggat gcaatcatag atttaattta
    13261 tctgatggaa tattgcttaa agacaaccat attggggctg ctggtagtgt taagaaggca
    13321 gttgaattag ctagaaaaaa tacttctttt gttagaaaga tagaagtgga agtggaaact
    13381 ttagacatgg taaaagaagc aatagaagct aatgcagata taataatgtt ggataatatg
    13441 agtttggaaa tggcaaaaca agcagtagat attataaatg gaagagccat tgtagaattt
    13501 tccggaaatg taaatttaga tactatagaa gatataggaa aaattggtgt ggatgttgtt
    13561 tctgtaggag cgcttacaca ttctgttaaa gctctcgata taagtatgaa gaatttgaga
    13621 aatatataag ttttatattg aaataaactt aattaaaata gactccttaa atttgtttta
    13681 taaatttaag gagtctattt tttattcatt aaaattaata taaatataaa aaaatcaatc
    13741 aataatttaa taaaaataga taaatactat atacactttt aataatatag ctaatatcta
    13801 ttttacagat aaataagaat aaacttataa tataattgta tctgtaaaat tttgtaaaat
    13861 ataaaaagta tagggggaat aaaaatgaaa gtagcttata tttttagtag tactaataca
    13921 cataaaattc tagacaaaat gataatacca caacttgaac aaggaactca tggagtagac
    13981 gttctaggga tgtttttctt tatggataat accttatttt tatctaaagg taatccagta
    14041 ggcgagagac taagtaaatt acatgaaaaa actgatatga taataatggc ttgtgaccaa
    14101 tgtgctatag aaagaggtat agagaataat ttggttgatg gagctacaat tggatgtttc
    14161 ccaaacctat atgcttgttt aggcagtgca ggtgtggacc aagttataac gttataaatt
    14221 agtataggat acaatccata gaaaaattta ttttctgtgg attattttaa gtaaaagata
    14281 aattgtttaa agtaggggat gaacaaatgt ctaaaatttt agttgaattt ataaacactt
    14341 gaccatcttg tgatgaacat ggagtgttta taaaaaaaat gtcagaaaag tatgctgata
    14401 aattggaaat aaaattatat caagcaggga aagatttttc gtatataaaa aaatatggga
    14461 ttataacaaa aggaacttta ataataaatc agaaaaagaa atatgataga ttaaataaag
    14521 atacaattga aagggcaata gtagaagcta taaacaataa ttaaaagttt aaaaattagg
    14581 agtatcaaaa atgaatatat tagaaattgt tttaaatgga gttaaagata ctatagtagc
    14641 ttctatagtt acactgaatg gagcatcagg atggcttatg ataagtttta tattggcagg
    14701 tgtacttcac aatgttttaa ctccaagaag gtttcaaaaa caacttggaa ataagaaatt
    14761 ttcttcttta attaaaagta ctatctcagg aatgttttta ccaatatgta gttgtggagt
    14821 aataccactt ggaataagta tgtattattc aggagcatat ttaggaccag tacttgcttt
    14881 tatgacatca actccaataa taaatccaat agctgtagtt ctttgtctag gtcttttagg
    14941 tcctaaagtt acaatcattt atctaatagc tggtttttta gtaccatttt tagtaggatt
    15001 tttaggaaat tgttttggaa aagaagaatt atcactttat aatgattcag aggaagaagt
    15061 aatagaatta gaagaagaaa aaagaagttt tatggaaaat atgattgatg gtcttaaatg
    15121 gtcttttggt gaattagccc ttactattag taaatatgta gtaatgggta tggtttttgc
    15181 aggatttata acaactattt ttccaaatag tataattcaa aaatatttag gaaatccagg
    15241 tttgctatct cttggaagta tagcaatact tgcatgtgtt atgtatgtat gtgctgtagg
    15301 acatataccg tttatagcag cccttgttgc aagtggagca tctcctggta tagcgataac
    15361 atttctaata gctggagcag ctacaaatct accagaactt ataagcatgt ttaaacttat
    15421 tggaaaaaga actgttatta tatattctgt aactttgaca ctgtcctcaa taataattgg
    15481 atatataaca aatttacttt tagtgaataa taattcagta aatttagatg caacaaattc
    15541 ttcaatacag ttggcaaata aactaatgtt aaatatacct gatagttttg agtatgtatg
    15601 tactatttta attattttat ttgcatttaa atcaacaata ccaaagatga aagaggtcat
    15661 aggaaatcat gctta
//
LOCUS       DAEPOZ010000078        15636 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.078, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000078 DAEPOZ010000000
VERSION     DAEPOZ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15636
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.078"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..426
                     /locus_tag="KR440_001804"
     CDS             <1..426
                     /locus_tag="KR440_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0698857.1"
                     /translation="FLKELSIDILNYPEYESNIVEFKSNIDNINFDFDCTLLKRAFSN
                     LIVNAFTYGDKNTKVCVHITLNERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKI
                     EGTGLGLAITKQIIEVHGGNISVESIPGLGSSFYITFPI"
     gene            646..3123
                     /locus_tag="KR440_001805"
     CDS             646..3123
                     /locus_tag="KR440_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBH0698858.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDVLTNLEDIPDLNAIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEMTENL
                     VNKLSEELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIENVRHMDGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDVVMIQIRDLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKKRSIAMYKSIGLSDK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            3138..3824
                     /locus_tag="KR440_001806"
     CDS             3138..3824
                     /locus_tag="KR440_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0698859.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALVGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            4097..4930
                     /locus_tag="KR440_001807"
     CDS             4097..4930
                     /locus_tag="KR440_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0698860.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCMQLKNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSLMYICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            4968..5429
                     /locus_tag="KR440_001808"
     CDS             4968..5429
                     /locus_tag="KR440_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBH0698861.1"
                     /translation="MKFGGVLLIVKDINKSKKFYEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFITQFQQL"
     gene            5582..6007
                     /locus_tag="KR440_001809"
     CDS             5582..6007
                     /locus_tag="KR440_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH0698862.1"
                     /translation="MHDELKIKMYSFTMDCKDPHELAKFYAALLNWEIAFYNEEYACV
                     GAPGTNQGTYPGITFQRNSEYKPPVWPEKPESQQQMAHMDFAVNDLEEAVQYEIHCGA
                     TIAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
     gene            6460..6780
                     /locus_tag="KR440_001810"
     CDS             6460..6780
                     /locus_tag="KR440_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HBH0698863.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
     gene            6918..7562
                     /gene="trmB"
                     /locus_tag="KR440_001811"
     CDS             6918..7562
                     /gene="trmB"
                     /locus_tag="KR440_001811"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBH0698864.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            7691..8437
                     /locus_tag="KR440_001812"
     CDS             7691..8437
                     /locus_tag="KR440_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698865.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEV
                     PKEALSLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLDFIVSVSDKSVLSCEDMIIKGN
                     KTGQAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(8566..8844)
                     /locus_tag="KR440_001813"
     CDS             complement(8566..8844)
                     /locus_tag="KR440_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698866.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     VLVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            9119..9565
                     /locus_tag="KR440_001814"
     CDS             9119..9565
                     /locus_tag="KR440_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698867.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            9835..10287
                     /gene="accB"
                     /locus_tag="KR440_001815"
     CDS             9835..10287
                     /gene="accB"
                     /locus_tag="KR440_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBH0698868.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            10303..11664
                     /gene="accC"
                     /locus_tag="KR440_001816"
     CDS             10303..11664
                     /gene="accC"
                     /locus_tag="KR440_001816"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBH0698869.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            11679..12533
                     /locus_tag="KR440_001817"
     CDS             11679..12533
                     /locus_tag="KR440_001817"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBH0698870.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKELLY
                     QILAMHKK"
     gene            12554..13498
                     /locus_tag="KR440_001818"
     CDS             12554..13498
                     /locus_tag="KR440_001818"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HBH0698871.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            13687..13947
                     /locus_tag="KR440_001819"
     CDS             13687..13947
                     /locus_tag="KR440_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HBH0698872.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            14100..14471
                     /locus_tag="KR440_001820"
     CDS             14100..14471
                     /locus_tag="KR440_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBH0698873.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIEDACKILSCNGYEVE"
     gene            14501..15347
                     /locus_tag="KR440_001821"
                     /pseudo
     CDS             14501..15347
                     /locus_tag="KR440_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         6053 a         1654 c         2564 g         5365 t
ORIGIN      
        1 ttcttgaaag aattatccat tgatatttta aattatccag aatatgaatc aaatatagtt
       61 gaatttaaga gtaatataga taatattaac tttgattttg attgtacact attaaaacga
      121 gcttttagta atcttattgt caatgctttt acatatggag ataaaaatac taaagtttgt
      181 gtacatataa cattaaatga aagaattgaa atcattatat ctgataatgg aaaagggatg
      241 tcaagagaag aagcatcaaa tttgtttaat cgttattata gaggaagcaa tacaaaacaa
      301 aaaatagaag gaactggttt aggactagca ataacgaagc aaattattga agttcatgga
      361 gggaatatat ctgttgaaag tatacctgga ttaggtagct cattttatat cacctttcct
      421 atttaattaa ggttaattaa ggtcaaataa aaaatatatt aaggttgata gattattata
      481 tttatatagt agtttatcaa tcttttttat ttaaggaggt gatttgatta aaaatattat
      541 aaatagaatg ataaaagtat ggaggagtct attagagatt caatttggat taaagtataa
      601 aaatattata ctgaaatatc ttagataaag gaagaaggtg taaatttgaa aattatatta
      661 aagtatattt tcacaaatat taaagaacga aaaattagaa cagcagttat gttactgtcc
      721 atagttttat caacagtttt actttttgtt tcattctcaa ttggattatc ttatgaaagt
      781 gcacaaagaa aaatggcaaa aggtatgtat ggtacagcga ctatatctgt ccaaagtaaa
      841 aatccagatg tattaaccaa tttagaagac atacctgatt taaatgctat aaaatcaaag
      901 gttggtgtat tggaaagctc tgctatttat aataaagggg gatactatga agaatttagt
      961 cttatatcag cagacttaag tcaactaaat aaaatcaaca agccacgatt agaaaatggc
     1021 gatagtatta ctgatttttc tggtgataaa attattcttc caaatcgctt tacatcaaaa
     1081 tataaaataa aaaaaggtga ttctattact ttacagattt atggaaagtc ttatactttt
     1141 caagttagtg atattgcatc ttatgataca gtatttttga ggaatacaag aggtgtaaat
     1201 gcactgctac ccaaagagac tctatcaaaa attataaata aaggcagtgg atatacaaga
     1261 gttcttatag agtcagaaga agaaatgaca gaaaatctag taaataaatt atccgaagaa
     1321 ctttccactg aaaaatatat ggtatcaaat actataaatg aaactaagat aatttcagat
     1381 gctagacaaa agacaatgcc tttcttttta ataagttttt ttgcattaac ccttagtata
     1441 tttatcatat acagcagcta taaggttatt acattagaac gtcttccttt tattggaaca
     1501 tttagaagta ttggtgccaa tgaaaaaact gtaactcgta tattgatgct tgaaagtata
     1561 ttatacggaa gtataggagg attaattgct attcctattg gtgtagttgt tttaaattta
     1621 atgcttcatg ggttaggaaa ttctctcgaa caaggaatat caattccagt agtaatatca
     1681 cccattggag tgattatatc tgttatagta gcaataatcg tttcttcatt tagtgcatat
     1741 atacctgtta aaaaagctag ccatttgcca ataaaaaata ttgtactagg aacagtagaa
     1801 gaaaagaatg tttcaaatcg ttctatactt tttataggaa gtataatgtt tattttatct
     1861 attttacttc caagaatatc ccctgaaaat acattgtatc tagctggtgg attttcatta
     1921 ctaggattaa ttgtagctac tattgtgttg attcctctta ttactgatat catgtctata
     1981 gtttttgagt ttgtttataa aaatatttta gggaatgagg gtaaattagc tgctagaaat
     2041 atgaaaaata acaaaaatat aatacagaat atcactttac tatttataag tatttctgca
     2101 gttattgcaa taagtgttgt tggtaacttt gtcaaaactt atataacaga tgtgtttcgt
     2161 gacgcagaat tacaaggttt tgcagatgga aagatgaatg aagagtttat tgaaaatgtt
     2221 agacatatgg atggaattaa aaaaatatta cctctttatg ttatgaataa tgagatttct
     2281 ggtaatggtg taacattatc aagactagag ggtacagata atataaaact atataactct
     2341 atgtttggaa tcaattatac aaattttgag ataaaaaaac aagtaataga agcttttaat
     2401 gataaacgtt ctgttatttt gaatgaagat actctaaaaa aagtaggctt atctatagga
     2461 gatacaataa ctctatctaa tgataagtat gatttttctt ataaaatagt aggtagtttt
     2521 aaatcccgtg caaatgatgt agaagctgtt ataccatcac attatgcagt taatgatttt
     2581 gataaaacta attatggttt tttagtatat acagcagtca atccagatgt tgttatgatt
     2641 cagataagag atttatttgg agatacatat aattggagtc gtacagttga agaatttaat
     2701 aatgattctt taaatactat aagtagtttt ttgtctccta tgaataagat gacctatttc
     2761 atttttcttc ttgctacagt aggtattatt aataatctac tgattaatta tattcaaaaa
     2821 aaacgttcta tagctatgta taaatctata ggactaagtg ataaacaaaa tataaaagtg
     2881 acattgattg aagggtttac ttcaggttta cttggagctg ttattggtat tgtgatatct
     2941 atacttgaga ttcaaactat ctttatagtt gcaggaccaa aaatttcaat gaaaccagat
     3001 ttagatttta aaacatttat tattgttggg ttattgggaa ttatagtaac tttaataggt
     3061 tcgatagtgc ctattataaa agggaagaaa atgaaactga tagaagaaat taagtttgaa
     3121 taattatgga ggttaatatg aaaattacac ataatagttt agctgtagaa gcaaaaaata
     3181 ttatcaaaga atataaaatt ggaaatacaa caactcgagt gttaaaagag gtatctttac
     3241 aagtaatgaa aggtgaattt gtttctatca tgggtcaatc tggttctggt aaaagtactc
     3301 ttttatatat tttaggtggt ttagatactc caacgagtgg aaaagtgtat atgaatggag
     3361 ctgatatatc acattttaat gatgagaaaa tgagcataat acgtcgtaga aatataggat
     3421 tcgtatttca attttataac ttaataccaa atttaaatgt ggaggaaaat ataatgcttc
     3481 ctttgttatt agatggtaaa aatttaaaag attataaaaa tcaattagat gaaatcttag
     3541 atatagttgg tttaactgat agacgtaaac atacaccacg agagttatct ggtggtcagc
     3601 aacaacgtgt tgctatagct cgagctttag ttggaaaacc agagatttta tttgctgatg
     3661 agcctactgg aaatttagat agtaaaacag ggatagaaat aatagattta ctgaataaaa
     3721 taaacagaga caatggacaa actataatta tggtaactca ctcaccagaa gcagccaaaa
     3781 gtagtagtcg tactattaca gtttcagatg gtttaattgt ataaggacta attattgtat
     3841 ttgagataag catttttaac tatataaatt agacatatgt aaagtggata taagttaaat
     3901 actataaatg tgctataata ttaaaataac agataaaagt actatatttt ttattgatta
     3961 ttttaattta tttgtttatt aaatatctaa tgagtattca tactatctct attattaaaa
     4021 gcagttaatg atatatagag ataatatgga ttcttcttac tttaataaat attcacgata
     4081 ggacaggata taatatatgt taaaaaaaga aagtattcat tttataaata aaaataggtc
     4141 acttatagtt tgtgaagaca attttgttta tctcttgcct tgtatgcaat taaaaaattg
     4201 gatatcgaat tttactatat cgtttccaaa tcaaacaata atttctgata attacacgat
     4261 tattcctcat ggaagtgtaa ctttggtgtt tttttatgat gccacagggt tacatagtct
     4321 attatttggt ccaactacta aaccaaaaac agtaggaaat attgctaata gatgtgatgt
     4381 tatactaatt atagaatttc aacctgctgg ttttttcccg cttataggta tacagcaaag
     4441 tgaacttatt gataaagttg ttccattttc tataattaat acttcattag atttagaaat
     4501 taaaaaaata tttaatgaat ctttatctat agataaattg attttaaagt tggaagaatt
     4561 attgatttct aatattaaaa ttgaatactc atatgagttt attttggcaa ttcagttgat
     4621 tattcaaaac tctggaaaca tatcttcaca agaaatttct aaaaaagtat tttatagttc
     4681 taggcattta aacagactct ttaatcagta cctagggttg agtatgaagt cattttctcg
     4741 tttggtacgt attaataaat ctataaaact tctaaacaat aataaaactt cattgatgta
     4801 tatatgtaat gaattaggat tctatgatat accacatttt attaaagatt ttaaaattgt
     4861 ctgtggaata actcctcaaa aataccgagc taatatgtct gatttttaca gtgaaattgc
     4921 aaaattttaa gttataatac ttataacaaa gaaaggatag tgataagatg aaatttggag
     4981 gagtattgct aattgtcaaa gacataaata aatcaaaaaa gttttatgaa gatgttatgg
     5041 agcaaaaaat tacattagat ttaggagaac atgtatcatt tgaaaatgga tttggattac
     5101 aaagtaatta taaagctata attggatatg atttaccaat atatcaacaa tcaaataact
     5161 ttcagttgta ttttgaggtt aatgatatca aacaatggga aagtaagata aatagaatag
     5221 gaaatattga atttttgcat tatataaaag aatacccatg gggtcaaaga acttttaggt
     5281 tttatgattt tgataaaaat attatagaaa tttcagaaag tatggaaagc gtcataaaga
     5341 ggcttttaaa acaaggttta gctttagaag aaatttcaaa acgcactatg tatccagttg
     5401 aatttattac acaatttcaa caattataaa atattaaaat atataatttt ttctttatat
     5461 tgcaaaaaac aaaggcttgg ggcataatag aaaaatactt gaaataaatt acatacttaa
     5521 tgtttgaatt tagcttaaaa atgaaaagac gaagaaattt atacttggag gtaattacgt
     5581 tatgcatgat gaactgaaaa tcaaaatgta ctcatttacg atggattgca aagacccaca
     5641 tgaattagca aaattttatg cagcgttact caattgggaa atagcatttt ataatgaaga
     5701 atatgcatgt gttggtgctc caggaacaaa tcagggaaca tatcctggta taacatttca
     5761 acgaaattct gagtataaac cgcctgtgtg gccagaaaaa cctgaatctc aacagcaaat
     5821 ggctcatatg gatttcgctg ttaatgattt agaagaagca gttcaatatg aaattcattg
     5881 tggagctaca atcgcagagg agcaatttac tgatgactgg agagttatga ttgacccagc
     5941 tggacaccct ttttgcttat gtcagatgaa atcaattatg gaaagtagcc atttttcatt
     6001 gttatagacc aaaatattgt ttattaatta caaaaaagca caacagtata taccgttgtg
     6061 cttttactta ttatacatga atacactaaa aaatataaat agtagagctt tatcaataat
     6121 gataattttt aaaaaatagt atttttttat tgaataattt cttagatatt ttaaagtata
     6181 taaattttgc atatatgaat tggacattat caaatgtgtt ataatattaa aacaacaaat
     6241 aaaattatta tattttttag aataataatt ttatttgttt actaagttta tgatagggta
     6301 tttatttaag gtaataaaat attatataaa atattttaaa gccttaggta atattaggta
     6361 gaagttttag taaaatatgc aaaaactaat tgattatgta ccttgtatgg gtatataata
     6421 gatattgttt taataaaata tttgaaaggg aatgatttga tgaaaattac tttaccagaa
     6481 acagctattg atactttaaa aagtatctta aaagacaatc aagataaacc taataatata
     6541 agagtatact ttgcaggtgt tggttgtgga ggtccttctt ttggattagc tttagatgag
     6601 aaaaaagaag atgacttaac ttatgaagtt ggagaattac aatttgtaat gagctctgat
     6661 gaatatagtc agtatggaga tataataata gaagatacag gatttggatt tagagttatt
     6721 cctgaaaata tgaaagatca aggtggagga ggatgctctg gttgttcagg gtgccactaa
     6781 gattaagttt ttagtaagtt tagaactctc tttatagaaa taaatttcta ttttaagaga
     6841 gttcttttta ttttttggtg agataatgta taataaaaat gtattttact aattttttat
     6901 atgtaaggag aattattgtg agaagaagac gaaaaaaagg tgcagatgaa aagcttttaa
     6961 gttatacaaa atatgtttta agagacgata tagacaaatt aaaaggcaag tggaatttaa
     7021 agtttagaaa tgataatcca atacatgttg aatttgggac tggaaaaggt aagtttataa
     7081 caactttagc taagcagaac cctgacataa attatattgc catggaactt aaggaagaag
     7141 ttcttttaaa agctgttgaa aaagcagatg cttctaattt gaataatata ctatttttat
     7201 ggggagatgt aagcaatatt ttggattact ttgaagctaa agaattatcg agaatttata
     7261 taaatttctg tgacccatgg cctaaaaata gatggagtaa aagaagactc actcattctg
     7321 gatttttaga aatgtacaat agggttttgg aagatgatgg cgaaattcac tttaaaacag
     7381 ataatgaaaa attattcgag tttagtttaa atgaaattgc tgcaaataat tggctattaa
     7441 agaatatttc tcttgattta ggaaatagtg agtatgaaaa taatgttaca acagagtatg
     7501 aagacaaatt tatgtcacaa ggtatgcgaa tatttagatg tgaagctaaa aaacgtaact
     7561 agtaatatgt ttataagttt aatagtagaa atttatttta atttatatta ataaagtgta
     7621 cgaaagtagg tatatataac aaggaaacag aaccctctca tagaaattaa attattataa
     7681 taaaaaattt atgtgggagg gtactatgaa tttagctaat ttaataccat ttattatttc
     7741 tgcagcagct ttaagtccaa taaattcaaa tgataaggtt ttgagcgaaa atcaacttaa
     7801 agatattttt cctgatgcaa atttaagagc agttgtaaaa agatatataa acccagatga
     7861 gatgactata agcaatataa aggctttaga tggagaattt tatgcaactg gtgagagcat
     7921 ttcaaattta aaaggaatat catatcttga aaatgtagac aattttattt tttggaataa
     7981 taatataaaa gaggttccta aggaagcttt aagtctaaaa gatatggatt caataaatct
     8041 tgccaataat tatttgattg atgacgatgt agtaaattct ctttctcata atggtgtgga
     8101 tgtaaattgc gatttaaatt ttattgatac aaaagataat cagtataaac tggactctaa
     8161 atatcataat gttgatatag caaaaggtga aagtattgat ttgagaaaaa taattacgaa
     8221 gaaaattgat agttattata aatactggga agttactgat aatcttccaa aagatttaga
     8281 ctttattgta tctgtaagtg ataagagtgt actttcatgt gaagatatga taattaaagg
     8341 taataaaaca ggtcaagctg tggtaaaagt tatactaagc gacaaaaatg atttaaatac
     8401 atctcaagaa gttctaatca ctgtcaatgt aaaataatat gtcaacttat tcaatcattc
     8461 tattttcaat ttaagtgtat aatcaaaata aaggttgtta gtaacagaac gctaatatta
     8521 gtgctttgtt actagcaacc tttaaatttt atagaaatag attatctaaa aaattccatt
     8581 tcttctgtta gtagcccata ttcctattaa aattatcagt gaatatatta tatacaaaac
     8641 tattcttata tattggacat ctaatccaaa aaattcaatg ttactctgtg aggtatgcaa
     8701 catcacaagg actgcagata taccaataag caaaggattg attactattt gcttatcagt
     8761 tcttaaaatc attattttac ccatccaaat tattgataat ataaaaaata aaattttaag
     8821 accaaattct aatgtatcta ccaaagcgaa aacatccctt catttataat aaatttttca
     8881 gctcaacaaa atataaaacc ctacaaacaa cttttgaatc cataataaga taacttgatt
     8941 aagcactatg aaataagtac tataaaactt catctacatt aatatatact acactatact
     9001 tcaaatttct taaacataaa ttaatgaaat taacaaaatt ttattcagaa acatataaag
     9061 atatatttta ctatttaaat gcatatcatt atatatacag ttttaggagg tgaggtatat
     9121 ggaatctaaa ttttttaatt ggtacactca atccttaggg ggaacttttg gcattatatc
     9181 atgtatatgc gcatatttaa atggatacat gtttgtttat acaaatagtg ctagcaaata
     9241 ttttgaaagt ataggatttc tgggaataat ttcaagttat acactacttc cactttgtat
     9301 gcttactttg atttttggag ttatatattc ttatactcca gaaacatccg acaaaagtat
     9361 ttttaatata ccttttttta ctttaaataa aagattaata cttattacag tagtttttgg
     9421 ttttataggt gctagagttt actttataat tcctgcaatt ctaataactt ttaacctata
     9481 tagtaatgtt ttgatagcta aattaaagta catcctatgt aaaaataaag aaaatgatac
     9541 taatttgaaa gaaagcgaag tctaaattgc ataatattgg tatttgtctt aaaaattccc
     9601 gaggtattaa tttgtattct cttgtatttt gcaaaaaata ttgaatttta ttcattttat
     9661 acttataatt tatattaata atggaaaatt aaaacaaata taagttactg gttgttaatt
     9721 taaaattaaa agatatattg atgtgttgat tctctagtca gcactttttc ttttataaat
     9781 ttgttttttg tgatataata ttagcaccag gtaataatac aagggggtaa caaaatgaac
     9841 atcaatgaaa ttaaggaatt attaaaggcc atagactcaa ctaacttaga atatgtaaaa
     9901 cttgaaagta gcgatttaag attagaagta tctaaaaaag ctcaatctac aagttcacct
     9961 gttttaagtg tacaacaaga atcagtagtg gatctttctt tagaaaagcc agttgtaaac
    10021 gatacacctg tcacatctaa tgagaatcta tcagtagtag tagctccttt gatgggaaca
    10081 ttctatgatt ctccaagtcc agatgcagat agctttgtta aagttggcga tgtagttgaa
    10141 gaaggcgaca ctttatgtat attagaagca atgaagctta tgaatgagat aacaagcgaa
    10201 attaaaggtg aaataattga agtattagtt agtaatgaag aactagtaga atataatcaa
    10261 cctttattta agataaaacc attgtagtag gaggtattta agttgataaa aaaaatacta
    10321 gttgcaaata gaggcgatat agcagttaga atcatcagaa catgtaaaga actaggtata
    10381 aaaactgttg ctatctattc tgaaatagat aaagattgtt ttcatagata tatagctgat
    10441 gaatctattt gtataggacc aaataatata agtaagagtt ataataatat agaaaatata
    10501 atctatcttg ctcttaaatt aaagtgtgat gctattcatc caggctttgg attcttatca
    10561 gagaatcctg aatttgcaaa gcaatgtgaa gataataata ttatttttat aggtccaaca
    10621 agagaacaaa tgattttgat gggagataaa tctagagcaa gagaaactat gatggaattg
    10681 aatatccctg ttgttcctgg ttcagaatct gttttgaaaa ctaaggaaga agctttagaa
    10741 gttgcaagag aaattggata tcctgttatg ataaaagcct caagtggagg cggtggaaaa
    10801 ggtatgagaa tcgttagaaa agaagaagaa cttttttcaa attttgatat ggcaagttca
    10861 gaagctctag cagcattttc taatagtgat ttatatatgg aaaagtttat agaaaatcct
    10921 agacatatag aagtacaagt atttggagat aaacattcaa atgcaataca tttaggtgat
    10981 agagattgtt ctatgcaaag gcgaaatcaa aaagtaatag aagaatcttt aagtccatac
    11041 ctttcagatg aagaaagaca aaaacttcat aaaattgcag tagatatagt aaaaggagta
    11101 ggttatatcg gtgcaggtac tattgagttt atagttgaca aagataaaaa cttttatttt
    11161 atagagatga atacaagaat acaagttgaa catccagtta cagagatggt aacaaatctt
    11221 gaccttataa aacttcaaat atctatagct aatggggata aaattccttt taaacaagaa
    11281 gatattactt ttagaggtca tgctattgaa tgtagaataa atgcagaaga ttcatcaaag
    11341 aattttgctc catcaccagg taagatagaa tcattaaatt tacctggagg ttttggtgtt
    11401 agatttgata cttttgtata tgcaggatat actataccac ctttatatga ttctatgata
    11461 ggtaagttaa tatgttgggc tgaaactaga gaagaatgta taagtagaat atatagagct
    11521 ctagacgaaa ttattgtaga aggtattaat acaaatgtag aatttcaaaa ggcacttgta
    11581 acaagtgagg agtttagaga agatactcat cataccaaat ttatagaaga tgtctttatg
    11641 aagaaagaat ttgctacatt ataaggagta aatagattat gataaaaaaa tttttatcat
    11701 caaaagaatc taagtatgtt acgatttctt tagatgacaa ctttaaaaag aatagtgtag
    11761 atgataaatt ttggacctat tgcaaaggtt gtgatagtca tgtatttaga aaggacatag
    11821 aagaaaattc atttgtatgt ccaaaatgtt caagacatta tggcttaaga gcaagaaaaa
    11881 gaataaattt attgattgat aaaggaactt ttatggagtt taattctgat gtagaatttc
    11941 aaaatccact aaattttcct aaatacaaag aaaaagtaga ctcttataag gaaaaaacaa
    12001 aagagtcaga agctgttgtc acaggttatg gaagaataaa tggaattaaa acagttattt
    12061 gtgtaatgaa tcctgatttt atgatgggaa gtatgggttc tattgttggg gaaaaaataa
    12121 cttattcaat agagtatgct gctgaaaata atttacctat aattatttgt tctgcttcag
    12181 gaggagctag aatgcaagaa ggtatggtat ctcttatgca gatggcaaag acttctcaag
    12241 cactttctaa gcttgaagaa aaatcattgc catatatatc agttctaact gaccctacta
    12301 ctggaggagt aactgctagt tttgctatgc ttggtgatat tataatttct gagccaaata
    12361 cgttgatagg ttttgcaggt ccaagagtaa tagagcaaac aataaatcaa aaacttcctg
    12421 aaggatttca aacttctgag tttttacttg aaaaaggttt tattgacatg atagtagata
    12481 gaagaaaaat gaaggaatta ttatatcaaa ttcttgctat gcacaagaaa tagggcataa
    12541 aggaggaact gttttgattg aaaataatag tgaaaaaata aaagtcttag aaaatgatat
    12601 taaacaatta ataactattt ctaaggcaaa taatatagat ttaagtgata aaataaattc
    12661 acttaatgag aaattggaaa agcttaagga agatgctttt tcacatctat cagcatatga
    12721 aaaggtaaca ctaagtagag atataaagag acctacgaca ttggaataca tagaacatat
    12781 ttgttctaat tttttagaac ttcatggaga taggcttttt aaagatgacc cttctatggt
    12841 tggagggatt ggtcagatag gaaagtttaa tgttactata gttggacacc aaaaaggaag
    12901 agatacaaaa gaaaatataa aaagaaattt tggaatgcct catccagaag gatacagaaa
    12961 agcattaaga cttatgaaac aagctgaaaa atttaataga cctataataa cgtttattga
    13021 tacatcagga gctttttgtg gtttagaagc agaagaaaga ggacaaggag aagctattgc
    13081 aagaaactta cttgaaatga gtaaactttc tgttccagta atcacttttg taattggtga
    13141 aggtggtagt ggaggagctt taggtatcgg agtaggaaat gatgtttgta tgctagaaca
    13201 ttcagtttat tctgtgattt ctccagaagg gctttctagt attctattta aagattcatc
    13261 taaagctaaa gaagcttgtg atgttatgaa gcttacaagt aatgatttat atgatttaaa
    13321 aataatagat aagattataa aagaacctct tggaggagca caaaaagatg tagaagctgt
    13381 ttcaaaagaa ataaaagctt atattttaga aagattaaat cattacaaag atatggataa
    13441 agaggaaatt atagctcaaa gatataaaaa atttagaaat attggaaaat gcttatagaa
    13501 tataaagaaa tttgtagttt tttcataata aaatggttaa aaaaaataaa ggttttctta
    13561 aaacgattat agaagtatat ttttataaaa gtatacttta aatgatgatt gtaattagta
    13621 aatggcaatt atgatttagt actttaggta atggatactt taacaaatta acaaggagat
    13681 gttgaaatgg aggtactaaa agtttcatca aaatctaatc ctaattctgt agctggagcc
    13741 ttggctggag tactaagaga acgaggcgtt gcagaaatac aagctatagg agctggagct
    13801 ctaaatcaag cgataaaatc aattgcaata gcaagaggtt ttgttgcacc tagtggaatg
    13861 gatttagttt gcattcccgc atttactgat atagaaatcg aaggtgataa gaaaacagcc
    13921 attaaactta taattgaacc tagataatat atctctttat tagaatttaa ttaagtttta
    13981 aaggttggct atagaaatag gacactatag tgaacctttt ttactttata tgttttattt
    14041 tatgtaactt ttaatgtata atcttaagta aacaaataag attggaggaa atatacatta
    14101 tgaaattaaa attattggta gaagagtatg cagtatgtag attaaataat gacagtaaaa
    14161 taccaacatg gatagataca gagaagtttt actctataac tagaactgat gatgaattaa
    14221 gtgttgtatg tttaaataat aatattcctt ctgatgttaa gtcagaaaag gagtggagaa
    14281 tacttaagat actaggtcct ttagactttt cacttgtagg aattttatct aagttaagta
    14341 gtttgttagc agacaacaaa ataagcatat ttgcaatttc tacgtatgat acagactata
    14401 tcttagtaaa agaaaaagat atagaagatg cttgtaaaat tttaagctgt aatggatatg
    14461 aagtagagta gttaaatact tttacatgag gacttaactt atgatttata taatcgaaat
    14521 tttaaaaata agtgtaataa atattttttt gtttcatagg aatatttatt gtatttggat
    14581 tgatttttag cattacagaa aatctaaata ataaatttat cttttcatct tttggaaaaa
    14641 ctggaattat tgtaactgga gctataggaa cgtttgttca tgaattgagt catttgataa
    14701 tgtgcttgat tttcatgcat aaaataaatt cagttaagtt tttcagacca atagaaagta
    14761 aaaatgatgg tatactagga tatgtaagtc atagctataa aaaagataat ctttatcaaa
    14821 gtataggaaa tttctttata ggaattgcac ctattatagg tggaacaata atgattattt
    14881 taatttttaa gatattactg ccagatagtt atatgaaagt gactcaaaat atagatttaa
    14941 agttatatgt aagtatgatt aataatttaa atataaatgg ttttatacac attattttag
    15001 gtgatatttc caattttata agaataatgt tgttaacacc tagtgtatat tcattgaagt
    15061 attttatatt tatgttttta atgtattcta tatctacaca tatgtcactt agtagtgctg
    15121 atttaaaaaa tagcttaaat ggtctgagct ttatagttat agttattttt gtaataagtt
    15181 caattacata cttatttgga tttgataact tgaatatatc cagtttaata gttaagtata
    15241 acatatttat aagtttcttt atggcaatag ggtttatttt ttcaatagta actctcataa
    15301 tatcctatat tttaagctta gtcagcccat ttaaaaactt tagttaaata aatacatatt
    15361 ttactaatat ttggtttcgt tagaaatata taaaaatatt atataaaaaa gattttagta
    15421 gagcataaaa aatttctcaa tatattttaa aatatttcta taaatatatt gagaaataaa
    15481 aaaagagtac ttaaattcaa ctagtcttta aatgtggatt tagggaatag ttagtgataa
    15541 tttcatctaa agcactgcta atctcttaaa aatgtaaaaa atgattaaat taaatattta
    15601 tactcacttg gtataaatat caaaaaataa tataaa
//
LOCUS       DAEPOZ010000079        15632 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.079, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000079 DAEPOZ010000000
VERSION     DAEPOZ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15632
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.079"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(34..2097)
                     /locus_tag="KR440_001822"
     CDS             complement(34..2097)
                     /locus_tag="KR440_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBH0698874.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFND
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     ILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYNNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNSIVD
                     KTYKRDFIDNIKSRDVYKLRKGIKISENIYLETNLNTEAKLNIIRFLLNEFDIELNEV
                     IFSIK"
     gene            complement(2478..5813)
                     /locus_tag="KR440_001823"
     CDS             complement(2478..5813)
                     /locus_tag="KR440_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBH0698875.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEKLRKSLTEKRKVNTVNYDFKIDEVNEFETRKRYINIELKLAGWE
                     FGKDIGEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMIDRRKSKKSLSSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKAETLTEKLFNIKVSMVKELQDIKYIEGKYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMVTAEKLSKLGTIPQIQQQKYTIEKAMTEDFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRKLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            complement(5863..6291)
                     /locus_tag="KR440_001824"
     CDS             complement(5863..6291)
                     /locus_tag="KR440_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698876.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDYLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            complement(6281..6661)
                     /locus_tag="KR440_001825"
     CDS             complement(6281..6661)
                     /locus_tag="KR440_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBH0698877.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYESKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            complement(6694..7002)
                     /locus_tag="KR440_001826"
     CDS             complement(6694..7002)
                     /locus_tag="KR440_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698878.1"
                     /translation="MNQIELDLYHDMVNIYKEADLQCNYKPTRFLQLINNKGALLAAK
                     ELINKEGATEGFTRLWECKRLDLSLEVLVLKEKYKELFTDEEREICINRLKDYGYKFN
                     "
     gene            complement(7035..8219)
                     /locus_tag="KR440_001827"
     CDS             complement(7035..8219)
                     /locus_tag="KR440_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBH0698879.1"
                     /translation="MVDEAKILDVVSISRENVKKFDGERSYLSTGNLDFNKISNLEIV
                     TYENKPSRANQTVNIGEVIFAKMKDTKKTLVINKTNKNIIVSTGFYVLKPSKEILPQY
                     LYHYLNSSYFLNQKNRLSKGATQSALNNEGLANIKIRMYNLKVQEKVVRVLDKAQELI
                     DKRKEQIEVLDELVKSRFIEMFGTPSKNEKNWEISEIGKYLDVLTDYHSNGSYETLRD
                     NVTLLDTKGYALMVRTTDLENNNFEKGVKYIDEHAYNYLEKSKVFGGEVIINKIGSAG
                     KVYLMPFLNKPVSLAMNQFMLRFNEDKVNHIFLYNLLLTSYMESKIKEKVRGAVTKTI
                     TKDAVRKINIIVPPIRLQNQFANFVKQVNSLKFEMETSLKELEDNFNSLMQKAFKGEL
                     FN"
     gene            complement(8223..9686)
                     /locus_tag="KR440_001828"
     CDS             complement(8223..9686)
                     /locus_tag="KR440_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HBH0698880.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFEKIFDDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KESAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIIGRVKVLEKEIVDGLEELERMLEV"
     gene            complement(10196..11065)
                     /locus_tag="KR440_001829"
     CDS             complement(10196..11065)
                     /locus_tag="KR440_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBH0698881.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIREVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIGTVKC"
     gene            complement(11085..13001)
                     /locus_tag="KR440_001830"
     CDS             complement(11085..13001)
                     /locus_tag="KR440_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HBH0698882.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNATKDGHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSGVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMISATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVSKLNNEEIKSV"
     gene            complement(13004..13924)
                     /locus_tag="KR440_001831"
     CDS             complement(13004..13924)
                     /locus_tag="KR440_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HBH0698883.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLADISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            complement(14026..14778)
                     /locus_tag="KR440_001832"
     CDS             complement(14026..14778)
                     /locus_tag="KR440_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH0698884.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            complement(15075..>15632)
                     /locus_tag="KR440_001833"
     CDS             complement(15075..>15632)
                     /locus_tag="KR440_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698885.1"
                     /translation="ISLILQSSLGSDAITLLNEGIHIKLGISYTIAGLIYNGTLLLIA
                     LLLNKKSLGLGSCVYVLVAGIFIDLYIYLLLDFNISSLGIIIRIMSFIIGHILMCSGF
                     AMLIKFDIGMSPLDAILIYIEGKFKYPYKVLKTIADLIFLILGVYLGGNLGFGTIFSI
                     LCTGTTISIVSKMISVNKKRYYSSI"
BASE COUNT         4908 a         2939 c         1520 g         6265 t
ORIGIN      
        1 atattctatt acgcaaaaaa tgcatatatt tcattatttt atggaaaata ttacttcatt
       61 taactctata tcaaactcat taagtaaaaa tctaattata tttaatttag cctctgtatt
      121 taaatttgtt tctaaatata tattttctga tattttaata ccttttctta atttatatac
      181 atctctagac ttaatattat ctataaaatc tcttttataa gtcttgtcta caatactgtt
      241 aaaccttaca gaatccaaat catataaagt aagagcaact tgttcataaa attctgtcca
      301 atttttagcc ttatatgact ctcctaatat actaaatgat actatacttt catttgtaaa
      361 attaacatca tcatcctcta acgtaaatat attttcactg ttacttggag gttcatatgt
      421 aacacttgga tatttccaaa tttctacagc tctatctagt aatatcttag ttctattttt
      481 tatttcttct tcattccatt tatctataga tgaaatatat ttatttagat aaagcctact
      541 atctttaaat ccatcttcca tttctttctt ttcttcaaat gttttattac ttagttttga
      601 attatatcct gtcaaagtta aatttccaat tgtatgtaaa tatttatcat gtatctcctt
      661 atagttatct cctagtgatt ccttccattt atttgttaat gtctgtggca ttatatgctc
      721 tatatttagc atattattat tgattaaatt ttctaaatca acaatttctt tattattata
      781 attctctaac ctctcaagta tatataactt attctttgac ttagtattat atatatcttt
      841 agtttcaaaa gcatttgaaa attcactgtc agtaggaaat ctttgagatg atttcttatt
      901 tataaaaata tatcttaata tattagaata gttttcttta taatcactaa attttttaat
      961 ttcccttcct aagttcataa atactttatt cagtgcattc ggaggtacat cacatactat
     1021 tcttcttaat ataaaacatt ctactaaatt aagggattct acaagtactt ttttatttat
     1081 tataccttca ttataatcat taaataattc taatataaac ggatatgata caaatatttc
     1141 aagtttgttt atccttttca aaactttgtt tacatcatta tcatcagtac atgaataaga
     1201 tatctctcta taatagttag aaaatttaag taaatctttt aataaaattt ctatatctat
     1261 accattatct gatacatacc ttttaaaact aaaatatact ttatttttat tcggtatact
     1321 tctctccttt atagtcaaat agtctcttat aaagctactt acgttgctac ttgtattttt
     1381 ttcaatttta taccaataat tattataaaa ttcctcttgt aacttagagc ttctacccat
     1441 taacacaaag tttcttactc tatcagcttc acttaaatct aagccagttg aatttagact
     1501 ttcaaagatt aattgagggt catcttcacc attttttaac tctatttcta ctatagttaa
     1561 tttctgtata gcatcaaaca aatcatctat tgatatatta tcttctttta ttctattata
     1621 aaaatataaa taatttgaaa ctatattaga atttctatct gtattcattt cttcttcaaa
     1681 tagttcatag taagcttttc tatcttctgc tattggtttt aactttattt ttccatcttt
     1741 atctcttgag tgcttgttta taagataaca ctccttaatc tgttcttctg ttgtaataat
     1801 tccttttgat tctccaaact cttttattat ttgacacatg gcaatcaata atattgacac
     1861 agtagtcact ctttgttgtc catctattat taaatattct tttcccatac tatatataga
     1921 tacaactgac cctaagaaat gagttctaaa tccagaatct attatatcct ctaaatcatc
     1981 aaacaatctt ttacattgtt ctttcttcca gttataattt ctttgatata caggcactac
     2041 aaaacttttg tttgacccct ctaaataact aagaaattca atttctcctg ctttcatatt
     2101 aactccccct ataagttaaa taaattccat tcataactcc atgtatctat atattatatc
     2161 aaaattttga ctcataaatg tatatgaaag ccttattata taccttttta tacctatttt
     2221 tattttatta aagtaaatac atgattattc tataaaatat acaaatactt aattttaaaa
     2281 attattattt aatcttcttc atgagtaatt catagttatt tatcaattaa aaagagatat
     2341 tgcataatta ttcaatacct ctctttgtat atttacatta tccatatcta gttatctatt
     2401 tcaacccaat attagactac ctactaataa atttattctc tacataatta acaataattt
     2461 tgcactaaat aacatcatta gcctatctct gatgcatttt ctttaatttt atttatagtt
     2521 ttaatcaatt tatttctctc ttcaatatga ttttcaaata aatcaattat acttccaaca
     2581 gttctaaatg ggtcttccat caacactttg ttatcctcta taaatccatt cttgactaca
     2641 taatcgacta taagtttaac aaaatgtatt tgcttagtat taaaactctt attatttaaa
     2701 aactctgaaa aagcttcatt tgcagcttct ctatccaatc ccactagttt tctcactaat
     2761 tgacttactg gagtatctcc aaactccttt ttataatcat cacaattacc taactgcttt
     2821 aagagtattt cctccaacgt ttcaacatct tgctttgtta tctttttatt atttcttaat
     2881 ttaaatattg ctaattcatc tttatggcta tttaaataat actctacctt ctttttataa
     2941 ttcttaagat tgtttgcatt atatacagaa tcatttctat cttctgctac aatcatatct
     3001 tcaaaacttg tataatatat cttttgacta gacttttcaa gatacttaat aagctctctt
     3061 aatgatatac gtacttcttc tacctcaaat atatctgaat cttcccaaaa atcctctgtc
     3121 attgcttttt caatagtata tttttgttgc tggatctgtg gtatagtccc aagtttcgaa
     3181 agtttttctg ctgtaaccat tacatgcctt atccctttac ttgcattatt attttgtaag
     3241 tatgcaagtt gtatattata cataactaca tcaaatcttt tagccaattc atcatcacta
     3301 tatgaagtta tgagtggtga tatatattct cttatctcat tttgtgctac tgctccaata
     3361 ttgctccaaa cattttcatt tttatactta tgtacataac ttaaattcat cctaactata
     3421 taactatcct catttaaagc ttttacagaa gttataagtt cttctaacaa ttcttttcta
     3481 agttctgcat acttaccttc tatatattta atatcttgaa gttctttaac catacttacc
     3541 tttatattaa ataatttttc agtaagtgtt tctgctttat ttccctcaaa tcctttagga
     3601 ttaaatttaa aaaattcaaa attattacaa aaatcaaata ttaagaatct ttccttatcc
     3661 atatcaacac ctaataaatc tggacataat ctagttcctc tacctatcat ttgccaaaac
     3721 ttagtcttac ttctcacttt cttgaaaaaa acaagattta ggatttcagg tatgtcaatt
     3781 cctgtatcaa gcatgtctac tgaaactgct atttgaggta atttattctt atcagaaaaa
     3841 tcatctatta tagaatctac ataatttata ctatagtcaa ctgcttttac aaagttccca
     3901 ccatactttg gatacagttt attaaatctt tcaactattg ccctagcatg ctttgtattc
     3961 tttgcaaaaa ttatagtctt accaatcttt tcttctcctt ctattttcaa cccctctgtc
     4021 attagcttat ttaaaactaa atctattgtg tcagaattaa atagccactc atttactgca
     4081 ttattcccta tttcgtctcc aatactctca tcatcattaa atgtattttc atattcttcc
     4141 ttctcttcat cagaaagctc atcatactta attccatctt ccataatctt agtcttaaac
     4201 tcaatactag tataatctac taaatatcca tcctcgactg ctttattata ctcataagca
     4261 tatgtaggta ctccattttc catgtcaaaa actgaataag tatttttatc tatctcatcc
     4321 tttggtgtag cagtaagacc cattaagtaa gaatcaaaat aatcaaagat tgacttatac
     4381 tttttataaa tacttctatg cgattcatct attataatta aatcaaaatg accaggtgta
     4441 aacaatctct ttccatcctt agacttcgta tcatcaattg cattcatcat tgttggatat
     4501 gtagaaaata tcatcctagc atcttctggg ctgtctttgc tatccaataa attacataac
     4561 gccaagttag gtaatagctt agtaaaactt tttttagctt gctttaccaa tgcttttcta
     4621 tctgctagaa ataatatatt tttaatccaa ttatgtcttg aaagtacatc aactatagat
     4681 atagcaattc ttgtttttcc tgtaccagta gcacatacca aaagagtttt gcgttgctta
     4741 ttttcaagag cctcacatac agccctaata gcttcttttt gataatatct attggatatt
     4801 tcatcattta ttgttacaga acttaaagac ttcttactct ttcttctatc tatcataagt
     4861 tgtaattctg attttttata aaatccataa acttttctat ctgaatagtc atcccaaata
     4921 tacatatcaa aaccattcgt caaaaatatt actggtcttt gactatatct cttttccaaa
     4981 caatctgcat ataatttagc ttgttgttga ccaattttcg ggtctttgct cgttcttttc
     5041 gcctcaacta ctgctaatgg cttaccattt tcaccataaa gaacataatc tacatatcct
     5101 acaccacttt cattaggcat accttgtact tctatttcct cccctatatc ttttccaaat
     5161 tcccaacctg caagctttaa ttctatattt atatatcttt ttcttgtctc aaattcgttt
     5221 acttcgtcta tcttgaaatc gtagtttaca gtattaactt ttctcttttc agttagtgac
     5281 tttcttaatt tctcattttc ttttattact tcttcaagct ttttatcttt tgaacttaat
     5341 ctctcataaa gatttttaag ctcgtctggt ctcgtccttt tctcttcccc atgaagtaat
     5401 aaatcttcat taaagtcttc tgctgtatat tcatctgaat aacaatagtc tatccaacta
     5461 acaaattgat gcaaattgtt aagtgaaagt attgcttctc ctctctctat gttcgcacta
     5521 gtatgtacag ataaattacc taatttaact atatatttta ataaaggtaa catatcatag
     5581 tctataagtt ctataaaact attattatga atcaacgtac taagattatc ttgataaggc
     5641 aatactaaat cactatcatt agcatatagc cattttactg caagctctaa agctcttctt
     5701 gttatagttg cacaagtaga tgggctaact agtatacttt tttctgcttc taagcaagca
     5761 gtattaaaac tattgaaatt tttcttcttt tgtaaaaagt caaaattacc caaggctttt
     5821 ctcccccttt tgtcccttag ttaaaataaa tctctctaaa aactatttct tacagctgtt
     5881 tcttatacta acaaatactt cttcattttc tgtaaacaat tcgtacatat cttcaaactg
     5941 tggtatatca tatccatgag aatcatcatt tctcaatttt ctaagtgttt ctataactat
     6001 agaagaattt ttaggtaata aattgtgata ctcacaagct aaaaggtagt cataaaagtt
     6061 tttagatttt atgtttaaat caattgattt ataatacaac attgcttttg ttaattctaa
     6121 aaatgagacc atatagcttt taaaaccttc ttttatgtat ctagcccata actcaggcat
     6181 ttcttcctta tcatataaga ttttgaagtt ttgcaggtca tataagcaag aaccaaaatc
     6241 cctatttact tcttctattg agttccaaga taatttgttt ctaattacca tacatgtccc
     6301 tccaatctaa gatatctatt acatcactgt tttcaaaata gaatttattt atataatcct
     6361 taaaatcttt agattcataa ataacttcac cttctaaaat agctttttta agtgctattg
     6421 tataattatc tgtaacaatt tttaggtcta catcaatgtc taatatattt cttagagctt
     6481 ctgtcttagt tgcaagttct gtaattggta cttttttgat aggaatccaa gctatgtcta
     6541 tatcgctttc tgatgtatgt ttatctgttc cataactacc aaatattatg attgctagag
     6601 tattttcttt tggaaataca tttattattg agtttttaat ctcatttggt atatttctca
     6661 tttaatcacc tcttgtttat taggtaatat tgcttaattg aatttatatc cataatcttt
     6721 taatctattt atacatattt ctctttcttc atcagtaaac aactctttat acttttcttt
     6781 taatactaat acttctaatg ataaatctaa tctcttacac tcccatagcc ttgtaaatcc
     6841 ttcagtagct ccttctttat tgattaattc tttagcagct aataaggctc ctttgttatt
     6901 tatcagttgt aaaaatcttg ttggcttata gttacattgt aaatcagctt ctttatagat
     6961 atttaccatg tcatgatata aatctagctc tatttgattc atagatttta ttcccccatt
     7021 ttaatacttg tatttcagtt gaacaattct cctttaaaag ctttttgcat taatgagtta
     7081 aagttatctt ctagttcttt taagctagtt tccatttcaa acttcaaaga gtttacttgt
     7141 ttaacgaagt tggcaaattg attttgaagt ctaataggag gtacaataat atttattttt
     7201 ctaacagcat ctttagttat cgtttttgtt acagctcctc ttactttctc ttttatttta
     7261 ctttccatat aggatgttag taaaaggtta tataagaata tatgattcac tttatcttca
     7321 ttaaatctaa gcataaattg attcatagct aaagatactg gtttatttaa aaatggcatt
     7381 aaataaactt tacctgcact acctatctta tttattataa cctcaccacc aaacaccttt
     7441 gatttttcaa gataattata tgcatgttca tcgatatact taacaccttt ttcaaagtta
     7501 ttattttcta aatctgtagt tcttaccatt aaggcataac cttttgtatc taaaagagtt
     7561 acattatctc ttaaggtttc ataactacca tttgaatgat aatcagttaa aacatctaaa
     7621 tatttcccta tttctgaaat ttcccagttt ttttcgttct ttgatggggt accaaacatc
     7681 tcaataaatc ttgatttaac taattcatcc aatacttcta tttgctcttt tcttttatct
     7741 attaactctt gagccttgtc taaaacacgc actacctttt cttgcacttt taaattatac
     7801 atgcgtattt ttatattagc taatccttca ttatttaaag ctgattgtgt agcaccttta
     7861 cttaacctat ttttttgatt tagaaaataa ctactattta agtaatggta taaatattga
     7921 ggcaatattt cttttgatgg ttttaaaaca taaaagccag tcgatactat tatattttta
     7981 ttagttttat taataactag tgttttcttt gtgtccttca tttttgcaaa tataacttct
     8041 cctatattta cagtttgatt tgctctagaa ggtttatttt cataagtaac tatttctaga
     8101 ttgcttatct tgttaaaatc taagtttcca gtactaagat atgagcgttc tccatcaaac
     8161 ttttttacat tttccctgct tatactaaca acatctaaaa tttttgcttc atcaaccata
     8221 ctctacacct ctaacattct ttctagttcc tctaatccat ccactatctc tttctctaac
     8281 accttaaccc tacctataat aacactagga gcatcataaa ctacttcctc atacactacc
     8341 tctttatact tattaataga taaatcatac ccattctctc ttatctcttc cacatcaaca
     8401 aaaaagcttt gctctgttct ttttctatct acttccccat caagattatc aaatctctta
     8461 ataatatcct ctatatcact atcctttatc tcatttctct tatcatccaa agaataacca
     8521 tcagctttca tatcatagaa ccatacctta tcagttccac ctgtccctgt tttagtaaat
     8581 atcattatag ctgtagatac ccctgcatat ggcttaaata caccacttgg cattgatatt
     8641 atagcttcca atttatgatt atcaactatt tcttttctta tcgatttatg tccacctgtt
     8701 gaaccaaaca atactccatc tggcactata catgcgcatc ttccaccaat ttttaaaact
     8761 cttaaaaaaa gtgccaagaa taataactct gttttctttg tcttagtcac ttttaataaa
     8821 tctgctgaca ctgcttcata atctaaactt cccttaaaag gaggatttgc tagcaccaat
     8881 gtaaacttat ctttatctgt attaacctcc gataaagaat ccttatactc tatattagga
     8941 ttgtcaacgc catgcaacat catattcata gcacctattc taagcatagt tctatccata
     9001 tcaaatccat agaacatacc attattaaaa tgctccttca gcccttgcac taagaaaaga
     9061 cttgaatgat tatcccttaa gtactgttgt gatgatacta aaaaacctgc cgaacctgct
     9121 gccgggtcta ctataatatc ttctggtgtt ggtttaataa gtcttactat catatctatt
     9181 atgtgtcttg gagtcctaaa ttgaccatta gtacctgctg tagccacttt ggataataaa
     9241 tactcataca aatctccctt tgtatctcta tccttactta attctaaatt actaatacca
     9301 tcaactattt tagataacat aagaggcgta ggaattttaa atattgcatc tcccatgtac
     9361 tttgcataag ctgattcttt atttccatgt aattttttta tgaaaggaaa cacttcattt
     9421 tgtactattt catacatttt ttctgagttt ccttcattag aaaacttact ccatcttaaa
     9481 tgttgatgtt catcatcaaa tatcttttca aaagaaacac ccaaaagagt tgcttctgct
     9541 tcattttttg tttctacttc atccaatcct ttaataaata aaagatatgt aaattgttct
     9601 ataacttcta atggattagt taatccacca gtccaaaatg tctcccaaat cttgtctact
     9661 ttactcctta attctcctgt aatcatatat gactttccct ccagtgttca taaaataatt
     9721 taaatatata ttttagtcgt acgccttaat agttagtgtc ttatgttaaa tccaagtttt
     9781 tactacacaa ttattaaatt atttttccca tttacatatt ttagctaaaa aactaatgca
     9841 atactcttat tttatgatta ttatatatac catacaaaat tattttatca tatatccatc
     9901 atagaagtca tctacctgga taactcttac catattataa ctttttaata ttaatttaaa
     9961 cttagaaaga aattttttat ttaattatat catcttcctt atttcaaatt tctcaactac
    10021 aatcctaatc aaaaaataaa ataagatgct gtctcaaaat agatataatc ttctttttta
    10081 ttgtcagaat gatgaaaata tatttgattt cacacaaaaa agagtgcctc atgaactaaa
    10141 aagttcattt tgagacaccc cttaatttaa tcttacttat tctttccctt tattattaac
    10201 acttcactgt ccctattgct tttcctacac atccacaaac ttccatccta ctcttaacaa
    10261 aatctttaac tgactctaaa ccttttttac catatttctt agggtcaatt acctctgggt
    10321 cagaattaag cacatcctta accccattgc tataagcaat ccttaattca gttgcataat
    10381 taactttgca aatacctctt tttatagatt ccttaataac ctcatctggt attccagacc
    10441 caccatgtaa tactaatgga actgatacaa cctctctaat ttcagataat ctatctaaat
    10501 caagctttgg ctctccctta tatagtccat gtgccgtacc tattgctact gccaaagatg
    10561 atacccctgt tctctctaca aactcttttg cttctaatgg gtctgtatat gctccaccat
    10621 caccaccatc taaatcgtct tccttacctc caactttgcc taattcagcc tcaacaggta
    10681 tatctgaagg catacatgca tctacaacag atttagatat tgaaatatta tcttcgtaac
    10741 tttcatgaga accatcaatc ataatagaag tatatccaac tctaagtgcc tgcatagcta
    10801 gtccaaagct tgaaccatgg tctaagtgca ttgccactgg aacacttgcc ttctctgatg
    10861 ctacctttac attcgctaaa taataatcta gccctgcata ctttattgtt gaaggtgttg
    10921 tttgcaatat tactggtgat ttaagttctt cagcagcctc tattactgcc ataaccatct
    10981 ccatattttc aacattaaat gcacctactg cataatgccc ttcttgtgca tctaataata
    11041 tttgtttagt tgtcactaat gccataattt ctcctcctat atgtttatac tgatttgatt
    11101 tcttcgttat ttaattttga tactatttct ctacttgttt tgctatttaa taactcattt
    11161 aaaaattctt cattcattaa ttttgaagat agttgagata atgtttttaa gtgtaaatta
    11221 tcaccattat ctgtagctga tatcataaat ataatatgtg ccaagcttcc atcttctgat
    11281 tcaaagtcaa acccttcttt tgatacacct actgctacac gtggtacctt tactgcatct
    11341 gtttttgcat gtggtatcgc tatccccata ccaaaacctg tagatgatat ttcttctctt
    11401 ttaaggattt cttctttaaa cttcttttta tctaaaagaa caccagattt atcaagtatt
    11461 ccaatcattt catcaattac atctgtttta ttttttccct taacatctaa aattactgtt
    11521 tcttcgctat ataaagagat attattagat acgttttccg attctgcact ttcattttta
    11581 tgtccatctt ttgttgcatt tgtattttct atagtatttt tctcatttac tactttaggt
    11641 gtaaccatca tataaagtac agcacctact aatgaaccta caaatataca ccctacccat
    11701 aaaagtggct tattgactaa tcctagaacc aaaaaccctc catgtggagc tggtacttct
    11761 attttcatta tataagttaa aattgatgat attgatgatg caatcataag tactggcaac
    11821 acttttaatg gattttttgc tgcaaaaggt attgccccct ctgttatatg agtactacct
    11881 agtatataat tgattaatcc tgctgctcta tcttcttctg taaatctatt tttaaataaa
    11941 gtagatgcta ttgctattac caatggtggt gttatacaag ctgcagatac acctgccata
    12001 aataaataat ttccctgtcc tagaagcata gttccagtta catatgctgc tttatttact
    12061 ggacctccca tatcaaatgc tgacatacat cctattacta ttcccaaaat tataggactt
    12121 gaattttgta accccgatag aaagttcatc atagcattgt taagtgctga acatggtcca
    12181 ccaagtaaca acattgctac acccacaaga cctacgctga caataggaat tataaaaatc
    12241 gatttaagac cttctaattg tcgtggtaat tttcttaaca aatgtacaaa tagtttaact
    12301 aagtaacctg ctaaaaatcc tgatatgatt ccacctaaaa accccgaacc tgtatcaaat
    12361 gatatcattc ctccaacaag tcctgccaac ataccagctc ttcccgatat agattgagct
    12421 ataaatgctg ccaatacagg aaccatcaag ttcatagaat atccaccaac agattttaat
    12481 gtagctgcta tttgattata ttgttctgaa tttgggtcaa atgaataaat cccccataaa
    12541 aatgatatag cagtaagtac tccacctgct actactaaag gtaacatgtg agatacacca
    12601 ttcattaagt ctttatacaa cttttgacca aacgataaat ttccttcttc tgcaatttta
    12661 ctgcttgata agtttccttt tctaatagct acttttccat caatacacct ttgtataagt
    12721 tcattagcct tatgtatacc ttctttgaca ggtacttcta ttaccggctt tccattgaat
    12781 ctgtccatat caacattttt atcacaagca actattattg catctgccat ttcgatatct
    12841 tttgctgtta acttattttc tacacctata gctccattcg tctcaacttt tatgtcacac
    12901 cctaattccc ttgctgaatt ttttaatgct tcttctgcca taaaagtatg agctattcca
    12961 gttggacaac ctgttacccc tagaatcatt ttctttccca tctttatacc acctttctga
    13021 ctcttatatt ttttatataa tcttccacat tctccaaatt acctatacca ttactttcag
    13081 ccacacttgc tcctgttgct gctgaccttt ttaatgcttc ttcaatatgt tctggatttt
    13141 ccaaccatat acttaaaaaa gcagctaatg cagaatcacc agcacatgca gagctgacta
    13201 ctgtaactgg ttgagcacta gcataataaa ttgattttcc attataaaaa taagaacctt
    13261 tttctcctaa agttagcaat atattttgag caccttttaa atgcagtaat ttaagtacat
    13321 ccttaatatc ttcttcatct cttacaataa ttccaaatat atctttgatt tcttcatcat
    13381 ttggttttat caaatatgga ttatactgta gtaactcctt taattttggt gaacttatat
    13441 cgagtattgt ctttatactt ttatttttac aaattttaaa tatatcctca tagtaattat
    13501 catcaatacc aggaggtaga cttccactta ttgataagta acttatatct gctaatgact
    13561 gtatcttatc tagcatatct agtttttgtt tttcgtttac atatgaccca gagtttacaa
    13621 atttaaattc atcacttcca tcatttaaaa atatatttat acgagttgta tcttctaccc
    13681 aagtaggtaa tacgtcaaat cctctctttt cgctttcttc aactatatat tttcctgaaa
    13741 atcctccaaa aaatcctaat atcttagatt ctataccata atgacctagt acaaaagtta
    13801 catttaatcc ctttccatta ggagaatata cagcatttga tgtacgattt actaattttc
    13861 tctttatccc attagtagat atattcatat ctattgcagg attcgtagtt aatgtatata
    13921 tcattgtctt cccccttgct tgtgctgtgt atgttttcat tataataaaa aagtctatga
    13981 aaatcttttc acaaatagaa aatcgatttt catagacttt tcaatttaat tttcagaatt
    14041 attatatctg accatataat ctataattat cctagatata atatacaagg gcaatctgga
    14101 agatacttca tattcagaaa ttgatatatg ttcatgtttg tatcctacaa tatataaatc
    14161 cgaatacttt ataagttttg cactttcact acaacagcaa gttataacct tagctccaac
    14221 taaattggca ttctgtgctg attctacaag ctctatggta tttccattca atgaaaatat
    14281 gattataagt tcgtcttttt ttattgattt agattttatt ttcattatgt tggggtcatt
    14341 tataaaaaca acattaaaat caagtaattg caatttaaga ctaaactctt ttgctacata
    14401 ttcagtaagc cctcttgcca aaatatatat cttactagat ttatttatag tttttattat
    14461 attgattagt gtagttaaag atatttgttc tataactctc tgtgcttcta taaaggattt
    14521 atttattata tcccccatat tttgtatatc atcattttca tcttttactg ttaatctata
    14581 cctaagttca ttgaatccat ttaaaccaca ttttctaatt gctcttgaaa cggttgctgg
    14641 agatgaatat gtttcatgag caatctctac tatagataat tctggtaata ttgattcatt
    14701 ttcatttata aatttaatta tttcaagttc cgtcttcgtt agattttcag aattactttt
    14761 gtttagctca attatcatat gttatctccc cttatataag ttttatttgt ctaaatttat
    14821 cgagtaatat aatatattac ctcttaacta cttattttta tatacaatta agatttagtc
    14881 aatgtctata tatgtaaatt tataatataa taattttatg cttcaattaa ttatgtaatt
    14941 attagagtat tttgtaataa aaattggagt tactcaatta ctgaaactga gtaactccaa
    15001 ttcttacagc ctcaaattaa ctcgaattat tgaaaatgat tcataaacat tatcctatac
    15061 tctcacttat caatttatat acttgagtaa tacctttttt tattaacaga aatcatctta
    15121 ctaacaattg aaattgtagt tccagtacat aatattgaaa atatagttcc aaaccctaaa
    15181 tttccgccta aatatacacc taaaattaaa aatattaaat ctgctatagt ttttaaaact
    15241 ttatatggat acttaaattt gccctcaata tagattaata tagcatctaa agggctcatt
    15301 ccaatgtcaa attttataag cattgcaaat ccactacaca taagtatatg tccaattata
    15361 aagctcatta ttctaatgat aatccctaaa ctacttatat taaaatctaa taataaatat
    15421 atatacaaat ctataaatat tcctgctact aatacataaa cacagcttcc taatcccaaa
    15481 ctttttttat tcaataacaa tgctataagt aatagagttc cattatatat tagccctgct
    15541 attgtataac ttatacctaa ctttatatgt ataccctcat ttaaaagtgt aattgcatca
    15601 ctaccaaggc ttgactgaag tataagagat at
//
LOCUS       DAEPOZ010000080        15536 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.080, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000080 DAEPOZ010000000
VERSION     DAEPOZ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15536
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.080"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..1825
                     /locus_tag="KR440_001834"
     CDS             <1..1825
                     /locus_tag="KR440_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698886.1"
                     /translation="KLLNNKNILFNKLKDIRNIQDTYSIILENKNEITKLKTSIEKIE
                     EEKKVIKKSIEDIDVNIFNLNSEKDRINRHIDDTESKINKFKIYEPIKLENAKLEELE
                     IKYKKYLEDPTKKEIETLNYEISVCLKRIESITIELEEFRQLISSDNFENIPILFTIE
                     ELNKRIVLNKSKVDELRKLKYSIKSDIDKNQGSLNTKREEILKKFNGKEPLSTSLIVN
                     FNFEERKIDIKKDIKDIGEIIEGNRTTCQNLEQELRDLKKYKVERKAITYQPEDIFIE
                     SKKLQSKLQNVEYNLIKSINSYKNTILEIKEFNYKHMEKYKNFIETLNVNKDNYSRQK
                     EQVEIVLLVIREEISKLEKHSEQLRKEKTIISRQIKDYVMDCIDEFKIINKLGKHKGQ
                     ALFNISLPKSDKIENSLSLVDMLMDEIVEEANLGDVEQKINTFYILNKTLNIGRIPVS
                     VIKYEINRNETIKWNSINNETTGGQRFCISFIVTILLMEYKRYDRNAIVDESKYKGKV
                     LLMDNPFGETSQQDFLKEIFELAKKFNVQIISYTHVTNASIRAMFNKIYLMTVEKTTS
                     NKEFVDINEIKQNKTDELVYMSKFYIGNKEELIQEDIFSLI"
     gene            1927..2136
                     /locus_tag="KR440_001835"
     CDS             1927..2136
                     /locus_tag="KR440_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698887.1"
                     /translation="MKNFVIVSTKAALLRVIIVRLLLKIGLKTYKYLSYFMKKEKLKR
                     IIRPEGCELRVNRSIQVERAIAEQI"
     gene            2532..2672
                     /locus_tag="KR440_001836"
     CDS             2532..2672
                     /locus_tag="KR440_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0698888.1"
                     /translation="MKIKIKKYREKLLMIQNELAKSVGVRRETIVHLENGLMYHKGIQ
                     DK"
     gene            2810..3772
                     /locus_tag="KR440_001837"
     CDS             2810..3772
                     /locus_tag="KR440_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBH0698889.1"
                     /translation="MAKIDNMMSILWMLNSDKKVTAKQISEKLEINIRTVYRHIDALS
                     ASGVPIISDTGHNGGYTLLNNFIKAPLFFDTEEQTALLHAAIFAKEEGSFLDDTLDKA
                     TSKLKMYSNQKQEETLTKHLIGFEVIKPTGKISIESVLKKLKNSIANELSVEINYRTA
                     HEEKSKCRIINPYGIVRWNNNWYIVAFCQMRNEIRSFRVDRISSVIETSNTFNRPKEF
                     SASEFFIRGIMDETKDEDNLISLVIEGKMNALDNLCQHWLLGYYLKERTSNKAIFKLQ
                     EEIIFTYVPNILIPYRKSIQIIEPLSLRDKMVEDLLELINHYQI"
     gene            3850..4479
                     /locus_tag="KR440_001838"
     CDS             3850..4479
                     /locus_tag="KR440_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBH0698890.1"
                     /translation="MKKRKVYMYVFDTMSDWEVGYLTAELNTGRYFKKGLKPLKVITV
                     GVDKNPIITMGGLKVLPEITIDELNIESNDLLILPGGNTWTSIGHEKILEKSKEALEQ
                     RSIVAAICGATLGLAKTGLLDFRNHTSNDLEFLKMVILSYSGEKYYKMESAVNDENLI
                     TASGIAPLEFTVQVLKVLDVFKEEALSSWLNLYKTHDSKYFFELMNCNK"
     gene            5310..5726
                     /locus_tag="KR440_001839"
     CDS             5310..5726
                     /locus_tag="KR440_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698891.1"
                     /translation="MSKSETGVKSKLILELLVITYLFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSKFILSKVNQIAITIMMYSIVIIAIFFATPYISNI
                     NISNLDTGIILIAVLFIQSLSKLILYIYFDKRGICN"
     gene            5727..5939
                     /locus_tag="KR440_001840"
     CDS             5727..5939
                     /locus_tag="KR440_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0698892.1"
                     /translation="MAISMKTKIKEYREKLLMTQNELAKSVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFGTTVENLFEFIEED"
     gene            6574..7617
                     /locus_tag="KR440_001841"
     CDS             6574..7617
                     /locus_tag="KR440_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBH0698893.1"
                     /translation="MKRKIAVVLLTVAMLLSLVACSNDNTSKSDKLEDTLVVYTTHGE
                     DMLGAISEEFTKETGVNVEFINLKGELADRVRSEKDNPQADIMFGGDTSTYMQLKEEG
                     LYEKTEPSWNNELDKSYKDSDGYWYGTIKTPVMMFYNTKLLSADKAPKDWSDLTKEEY
                     KDLIVTRDSLSSSMRSTISNLINYYSNTKSEEEAWKYLEDLNKNIKNYYNSGSVMYSA
                     IGKGEATISVAVLSDIIENKEKNNMPLEVVDAESGSVVITDCVAALKNAPHPNAAAKF
                     VDFVGSAKVQAMVANDFNRIPTLDSALKDSPKWMSKEYKAMDVDWSVIGKNQSNWIEK
                     WETDIIDNNKTIK"
     gene            7878..8752
                     /locus_tag="KR440_001842"
                     /pseudo
     CDS             7878..8752
                     /locus_tag="KR440_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421888.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8771..9763
                     /locus_tag="KR440_001843"
     CDS             8771..9763
                     /locus_tag="KR440_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0698894.1"
                     /translation="MKNIELKEINHYYGKEKVLHDINISIKEGEFFTLLGPSGCGKTT
                     ILRIIGGFIKPSSGSIYVGDKNITNLEPENRNMGTVFQNYALFPNMTVEENVSYGLKI
                     KKLSKKIIKEKCDNYLELSGMKDFRKKKIDELSGGQQQRVAVARSLATEPTMLLLDEP
                     MSNLDIALRIKMREEIREIQQKTGITTLFITHDQQEALAISDRIAVMDKGKVLQIGVP
                     IEVYKSPLNDFVANFVGTSNCIGKEDYVNFNIEKETKPYIYKRPEEMILLQNTNQSGF
                     VKVKVESKNFLGAILEYTVSNDGKKYQVTELNRLNNSNKLNVGDMAYLGVLQGD"
     gene            9765..11459
                     /locus_tag="KR440_001844"
     CDS             9765..11459
                     /locus_tag="KR440_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBH0698895.1"
                     /translation="MIKKINRSKFTSKNVIFIVLYSILFWILIGYIIIPIGNTFVQAF
                     QGKGGYSFEVFKEYFSNTNNLRVVSNTIILGLGSVFVCGIMGISLALYMTFVCDKHKK
                     IIHILLLSPMMIPGVIMVIACIQIYGESGIVTKSIEILLPFIKKPYDFSGLKGILFVV
                     AYTQYVYFYLNVYVALKYVDYSTVEAAKGMGASKLRVFFDVIWPVITPAVITSTIITF
                     ASGISSFSAPNLIGGGFKVLSTQIVRSKANNHMDIASVQVITLLLMGILFMILLQYYG
                     KKYGVVPSLKSQSYKVHNRKKDLFTFVCKVIIEIQIIMIMLPIVTIVYLSFVSTHSIM
                     TTIFPRDFTLENYQMIFQKSRVLQPILNSFKMSLMAVAVGLIITVPVSYLVIKKDTAY
                     NRLAKFIIMLPWSMPASAIAVNLINTFNQKSIFAFNESLIGGFYIVPIAYTIYALPLL
                     FSSNEVAMKSINLGLEESSRSLGAGRIKTFYNVIVPNMMPGIISGAILVFIRTIGEYT
                     MSALLYGVYNRPISISIVTNMQEFKIGVSMAYGVLVIGICYIALGIIFKLDRKRFT"
     gene            12010..12888
                     /locus_tag="KR440_001845"
     CDS             12010..12888
                     /locus_tag="KR440_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBH0698896.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQGANEKNAPV
                     ILAIHPNELSFLGDSFVVSCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVDTLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKAAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            12924..14819
                     /locus_tag="KR440_001846"
     CDS             12924..14819
                     /locus_tag="KR440_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0698897.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSETKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFPPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVAKPTIRSENS
                     EVELLGA"
     gene            15087..>15536
                     /locus_tag="KR440_001847"
     CDS             15087..>15536
                     /locus_tag="KR440_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBH0698898.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGT"
BASE COUNT         6300 a         1621 c         2638 g         4977 t
ORIGIN      
        1 aaagctatta aataacaaga atatattatt taataaattg aaggacatta gaaatataca
       61 agatacatat tctataattt tagaaaataa aaatgaaata actaagttaa aaacaagtat
      121 tgaaaaaatc gaagaagaaa aaaaagttat taaaaaatca atagaagata ttgatgttaa
      181 tatatttaat ttaaattcag aaaaagatag gataaatagg catatagatg atacagaaag
      241 caagataaat aaatttaaga tttatgaacc tattaaactt gaaaatgcta aactagaaga
      301 attagaaata aaatacaaga aatacttaga agaccctaca aaaaaagaga ttgaaacatt
      361 aaattatgaa atctcagttt gcttaaaaag aatagagagt attactatag agttagaaga
      421 atttagacaa cttattagta gtgataattt tgaaaatata cctatattat tcacaataga
      481 agaacttaat aaaagaatag ttttaaataa aagtaaggtt gatgaactac gaaaacttaa
      541 atatagtatt aaaagcgaca tagataaaaa tcaaggttca ttaaacacta agagggagga
      601 gatacttaaa aaatttaatg gaaaagaacc tttatctact agcttaatag tgaactttaa
      661 ttttgaagaa agaaaaatag atataaaaaa agatataaag gatattggtg aaattataga
      721 aggtaataga actacttgtc aaaatctaga acaggaatta agagacttaa agaaatataa
      781 agtagaaaga aaagctataa cctatcaacc tgaagatatt tttattgaaa gtaaaaaact
      841 acaatcaaaa ctgcaaaatg ttgaatataa tttaattaaa tctataaata gctataaaaa
      901 tacaatatta gaaataaaag aatttaatta taagcacatg gaaaaataca aaaactttat
      961 agaaacttta aatgtaaata aagataatta ctctcgacaa aaagaacaag tagaaatagt
     1021 attactagtt ataagagaag aaataagcaa attagaaaaa catagcgaac aactaagaaa
     1081 agaaaagact ataatatcaa ggcaaataaa agattatgtt atggactgta ttgatgaatt
     1141 taaaatcata aataaacttg gtaaacataa aggacaagcc ttattcaaca tatcactacc
     1201 taaatctgat aaaatagaaa atagtttgag cttagtagat atgctaatgg atgaaattgt
     1261 agaagaagca aacctaggtg atgttgaaca aaaaataaat accttttata ttttaaataa
     1321 gactcttaat atagggagaa ttcctgtatc agttataaaa tatgaaataa atagaaatga
     1381 aactataaag tggaactcta ttaataacga aacaacagga ggacagagat tttgtatctc
     1441 atttattgtg acaatattgt tgatggaata taaaagatat gatagaaatg caatagttga
     1501 tgaaagtaaa tataagggta aggtactact tatggataat ccttttggag aaacgtcgca
     1561 gcaagatttc cttaaagaaa tatttgaact tgctaaaaaa ttcaacgttc aaattatatc
     1621 ctatactcat gtaacaaatg cttctataag agcaatgttt aataagattt atttaatgac
     1681 agttgaaaaa actacttcta ataaagaatt tgtggatatt aatgagatta agcaaaataa
     1741 aactgatgaa ttggtgtata tgagtaaatt ttatataggt aataaagaag aattaattca
     1801 agaggatata tttagtttaa tataaataac aagtaaacaa ataaagtgat tactatcagt
     1861 gaggcaggta gaaactgaat tactttgtga ctcattacga ctcttggggt aaaattgtgt
     1921 ataaatgtga agaatttcgt gattgtcagt acaaaagcag ctttattaag ggtaataata
     1981 gtaagactcc tcttgaaaat aggactaaaa acttacaagt atctaagtta tttcatgaaa
     2041 aaagaaaaat taaagagaat tataagacca gagggatgtg aacttagagt gaatagaagt
     2101 atacaagtag aaagagctat cgcagaacaa atttaatttt atcatacgat agcctcttgt
     2161 tattttagtg atgaaatcca tcaagcatac cataactaca gaaaaagagg cttttctgat
     2221 ggtatattgg gttcttttag agtgtgtgat ttgctacatg ttcattagga taataatagt
     2281 cagtaaatga tattactgat agaatagata taataaacta atctagttcc atattgtaat
     2341 gtttattaaa gagtatactt ttatgattat aatagctatt ttctttgaag cttattgtac
     2401 aagagatttg aatatatcaa atttggatat aggtataatt ctcttagcag ttttatttat
     2461 acagtcttta tctaaattag tgttgtatat actttgataa aagggggata tgtaactaag
     2521 ttgtaatatt gatgaaaata aaaattaaaa aatatagaga aaaactcctt atgatacaaa
     2581 atgaacttgc taaatcagtt ggggtaagac gagagactat tgtacactta gaaaatggat
     2641 taatgtacca taaaggtatc caagataaat agttaaaaaa ctagataaga ataaggagcc
     2701 tttacagaac aaatttagtg tgctcgtgcg atagcccttt ttttataaaa attaatctaa
     2761 taacctatat aattaaattt ataaaacata tatgaaaggg taaaactata tggctaaaat
     2821 tgataatatg atgtccatat tatggatgtt aaactcagac aaaaaagtta ccgcaaaaca
     2881 aatatctgaa aaactagaaa taaatataag aactgtctac agacacattg atgcattatc
     2941 tgctagtgga gtacctataa tttcagatac aggtcataat ggtggatata cacttttgaa
     3001 taatttcatc aaagcacctc tttttttcga tacagaagaa caaactgcgt tacttcatgc
     3061 agctatattt gctaaagaag agggaagttt cttagatgac acactagaca aagcaacctc
     3121 aaagttaaaa atgtattcaa atcaaaaaca agaagaaact ttaacaaaac atttaatagg
     3181 ttttgaagta ataaaaccaa ctggaaaaat atccatagaa tctgtactta aaaagttaaa
     3241 aaatagtata gcaaatgaat tatctgtaga aatcaactat cgtacagctc atgaagaaaa
     3301 atcaaaatgt aggattataa atccatatgg aattgtacgt tggaataata attggtacat
     3361 agtagcattt tgtcaaatga gaaatgagat tcgtagtttt agggtagata gaattagtag
     3421 tgtcatagaa acatcaaata cttttaatag gccaaaagaa ttttcagcaa gtgaattttt
     3481 tataagaggg attatggatg aaacaaaaga tgaggataat cttatatcat tagttataga
     3541 aggaaagatg aatgcattgg ataacttatg tcagcattgg cttctaggat attatttaaa
     3601 agaaaggact tcaaataaag caatatttaa acttcaagaa gagataatat ttacatatgt
     3661 accgaatata cttatacctt atagaaaatc tatacaaata attgaaccat taagccttag
     3721 ggataaaatg gttgaagatt tactcgaatt aattaatcat tatcaaattt aataacttaa
     3781 ctgacagagt atgtcagtta agttatttta caattaggta tattaattaa ttatagaagg
     3841 agcaaaaaaa tgaaaaagag aaaagtatat atgtatgtat tcgatactat gtcagattgg
     3901 gaagttggat atctaactgc tgaattaaat acaggaagat attttaaaaa aggattaaaa
     3961 ccactaaaag taattacagt aggagttgat aaaaatccta ttataacaat gggtggatta
     4021 aaagttttac ctgagattac aattgacgag cttaatattg aaagtaatga cttattaatt
     4081 ttgccagggg gaaatacttg gacatctata ggtcatgaga aaatattaga aaaatctaaa
     4141 gaggctttgg aacaaaggtc tatagttgca gctatttgtg gtgctacttt agggttagct
     4201 aaaacaggtt tactagattt tagaaatcat acaagtaatg atttagaatt tttaaaaatg
     4261 gtgatactaa gttattctgg agaaaaatat tataaaatgg aatctgcggt taatgatgaa
     4321 aatttaatta ctgcttctgg aattgcacca ttagaattta cagtacaagt tttaaaagtt
     4381 ttagatgttt ttaaagaaga agcattaagt tcttggttga acctttataa gacgcatgac
     4441 tctaaatatt ttttcgaact aatgaattgt aataaataaa tttaagattt aattttaaat
     4501 tcaaatttta aattactttt tctatggcaa tagataagta gtttagaatt ttatcatata
     4561 tataatttgt atttatacca acacaaatag aaatgatagt tccaataata aaatttataa
     4621 ttagttccat atggagtaca tttctttcct actaatataa ctaagaatat atgaaaccac
     4681 catagattgt agttttttct gttgtattat atcatgggat agtaaagtga cgtatatttt
     4741 tgatgaaaac ctaggattgt atatgaaatg aattaatcct gtgtattaat cttgagattc
     4801 gtgtctaatg atttgtaaaa ataaataatg aaatacttag aaatacttgt aaatgctagt
     4861 caatattggc tagtattttt tattttagta atgaaatccc caactgtact tcatccacaa
     4921 aaaaagagat atttttgagg gtgtattgga gtgaacttag gctgtataat tatacatttc
     4981 aatatcttat attaattagg ataatagttg gtaaatgata tgattgaaac aatagatata
     5041 ataaactaat ctagtactgt actgcttact aaagaatata tttttatggt ataagaattt
     5101 tttagagcgt tgattctata ggagtgaata aaaaagtaat tttataatat tatttgtaac
     5161 cattagatgt ctattatgaa atcttttatg gactatattt gtaaaaatat tattaagtat
     5221 ttacaatatt ttcttgcaat ttatttggaa aggttatata tttataacat gaagtgttat
     5281 aaatatataa cctttaaggt ggattttata tgtctaaatc agaaactggt gtaaaatcaa
     5341 aactcatatt ggaattactt gtaataactt atctttttat agctgtatta gctactaagt
     5401 atatttttaa aggtccaagt actgcatctc ttccaggact gagctggtta atagtctttg
     5461 gatgctattc aatttataat aaaaaatata atgatttagt tgatgaatca tctaaattta
     5521 tactatcaaa ggttaatcag attgcaataa caataatgat gtattctata gttataatag
     5581 ctattttctt tgcaacacct tatataagta atataaatat atcaaacttg gatactggta
     5641 taattcttat tgcagtttta tttatacaat cgttatctaa attaattttg tatatatact
     5701 ttgataaaag ggggatatgt aactaagtgg caatatcgat gaaaacaaaa attaaagaat
     5761 atagagaaaa actccttatg acacaaaacg aacttgctaa atcagttggg gtaagacgtg
     5821 agactattgt acacttagaa aatggaaaat ataatccttc tttaaagtta gcgatggata
     5881 ttgctaaagt attcgggacc acagtagaaa atttgtttga atttattgaa gaagattagt
     5941 agataggata taaatattta taagattgtt aataaaatat agttatttaa ttattaattt
     6001 tgttaaaaat atttataaat aatataatgt atgagtaaag ttaggttgta attattttaa
     6061 gataagaaga ctactgtaaa tgataaaaat tagtgaatat acagccaatt aataaataat
     6121 agataaaact ttaaagctta gatgtgagga tgagatatgg tcttacagta gttcacactt
     6181 ttaattaaga cttaaaatgg aaaccataca catacataca ctatttactg aaagtggact
     6241 agataaaaaa ataataagtg atttaagaaa gtatctcata tactatataa gtattatttt
     6301 aggtgaccta tttataggtc attttttatg tatataaatg ataataatac taatatttaa
     6361 aataggtttt aaagaaataa aatatccaga ttgaaaaatg gattaatgtt aataaaaaaa
     6421 ttatatgtat aaaaataggt ataattatag aaaaaaacaa taataaaata agctgttata
     6481 gaaaaataca attatataga ttgttgttga atcattgact gcaagaataa taacatttaa
     6541 aatgaacatg gagaatatgg gggaggaatc aatatgaaga gaaaaatagc agttgtttta
     6601 ttaactgtag caatgttatt atcacttgta gcttgtagca atgataacac atcaaaaagt
     6661 gataaattag aggatacatt agtagtatat actacacatg gagaggatat gctaggagca
     6721 atttctgaag aatttacaaa ggaaacagga gttaatgttg aatttataaa cttaaaaggt
     6781 gaactagcag accgtgttag aagtgaaaaa gataatccgc aagcagatat aatgtttggt
     6841 ggagatacat ctacatacat gcaattaaaa gaagaaggat tatatgaaaa gactgaacca
     6901 agctggaata atgaattaga caagtcttac aaagattcag atggatattg gtatggaact
     6961 ataaaaacac cagtaatgat gttttataac acaaaactat tatcagctga taaagcgcca
     7021 aaagattgga gtgatttaac aaaagaagaa tacaaagact taatagttac tagagattca
     7081 ttatcatcat ctatgcgttc tactattagt aacttaatca attactactc aaatactaag
     7141 agtgaagaag aagcttggaa gtatcttgag gatttaaata agaatataaa gaattactac
     7201 aatagtggaa gtgtcatgta ttcagcaata ggtaaaggtg aagctacaat atctgttgca
     7261 gtattaagtg atataataga gaataaagaa aaaaataata tgccattaga agttgtagat
     7321 gcagagagcg gttctgtagt aatcacagac tgtgtagcag ctctaaaaaa tgcaccacat
     7381 ccaaatgcag cagctaaatt tgtagatttt gttggaagtg ctaaggttca agctatggtt
     7441 gcaaatgatt ttaatcgtat tccaacatta gattctgcac ttaaagattc accaaagtgg
     7501 atgtcaaaag aatataaagc tatggatgta gattggtctg taattggaaa aaatcagtct
     7561 aactggattg aaaagtggga aacagatata atagataaca ataaaactat aaaatagcct
     7621 aattaaataa tttaatatta tatacaaaaa tagtgaggat tttcctcgct attttacgta
     7681 taaggaaact atagtatctc ataattttgt ataattcata caacaaaatg atactaatac
     7741 atacttatta aataaaatag tttttgagta actgatgaag taattgacta tataaagtga
     7801 tttgtagaca tagtctctca aataaagtga attttttatt atgcaaaact tttaattata
     7861 gtaaaagtac aatttatatg ggcaaggaga taaaaatgga actaaaaatt agtgattata
     7921 aattattatg taataaaggt agtggcgctt ttaacgaaga tatcgtaggt ttatcacctt
     7981 atggggcatg ggtacttgat ggagctacag gtttaaataa taaaaattta gtctctacag
     8041 aaagtgatgc caaatggtat gtaagttggt ggaatagtta tcttcatgag aatatggaaa
     8101 gagaagatag cttaaaagta attattaaag aaggcattga ggagataaca aaagagtact
     8161 acaaattaac agatgataaa gaagtagaag caatagatat tccttcttgt tcatgttcta
     8221 tattaaagtt ttataaagat aagatagagt atttactttt aggagattgt actttattag
     8281 ttaacaataa tgaatctgtg aaaattttaa aagatgagac tttatgtgaa cttgataaaa
     8341 aagtatttga ttttatgaaa gaagccaaag atgctacaaa ccttacttta aaggagagaa
     8401 aagccgaggt aatgcgatta taatagagaa tagacttaag aaaaatactg tggggggata
     8461 ttggactttg gaattttcta aagaggccgt tgacaattgt gtaagtggat ttattgatat
     8521 agaatataaa atgaaaatta tgatggctag tgatggtttt tcatgtggat atgatagata
     8581 taagattttt gataaaaatg aaatgatgaa aatagcagag attaaaggta tagattatat
     8641 atatagaaag gttagggaac tagaaaagga tgatgaaatg gcaatagagt ttcctagatt
     8701 taaaatctat gatgattcat catgtgtata tttagagatt tataaatatt agataattta
     8761 tggagacgat atgaagaata tagaattaaa agaaattaat cattattatg gaaaagaaaa
     8821 agtattacat gatattaata tatctattaa ggaaggagag ttttttactt tactaggtcc
     8881 ttctggatgt ggaaaaacga ctattttaag aataattggt ggatttataa aaccttcttc
     8941 tggaagtatt tatgttggtg ataaaaacat taccaattta gaaccagaaa atagaaatat
     9001 gggaactgtt tttcaaaact atgcactgtt tccaaatatg acagttgaag aaaatgtatc
     9061 ttatggattg aaaataaaaa agctgtctaa aaaaattata aaggaaaaat gtgataatta
     9121 tttagagctg tcaggaatga aagattttag gaaaaaaaag attgatgaat tatctggtgg
     9181 acagcaacaa agggtggcag tagcaaggtc attagcgact gagcctacta tgcttttatt
     9241 agatgagcca atgtctaatc tagatatagc tttaagaatc aagatgagag aagaaataag
     9301 agagatacag caaaagacag gtattactac tttatttatt acccatgatc aacaagaagc
     9361 cttagctatt tctgatagaa tcgctgtaat ggataagggt aaagttcttc aaattggtgt
     9421 gccaatagaa gtttacaaaa gcccattgaa tgattttgta gcaaactttg taggtacttc
     9481 aaactgtatt ggaaaagaag attatgttaa ttttaatatt gaaaaagaga caaagcctta
     9541 tatttataaa agacctgaag aaatgatatt attgcaaaat acaaatcaaa gtggttttgt
     9601 aaaagtaaaa gtagaatcta agaattttct aggagcaatt ttagaatata cagtatcaaa
     9661 tgatgggaaa aaatatcaag taactgagct taatagactg aataatagca ataaattaaa
     9721 cgtaggggat atggcatatt taggtgtgct acaaggtgac taatatgatt aaaaaaataa
     9781 accgtagtaa atttacatct aaaaatgtta tttttattgt tttatacagt atattatttt
     9841 ggattttgat tggatatatt atcataccaa taggcaatac ttttgtacaa gcattccaag
     9901 gtaaaggtgg atattctttt gaagttttta aagaatattt ttcaaatact aataatttaa
     9961 gggtagtaag caatactata attttgggat tgggttctgt gttcgtatgt ggaattatgg
    10021 gaatatcact tgccttatat atgacatttg tttgtgataa acataagaaa atcatacata
    10081 ttcttttact aagtccaatg atgattcctg gagtaattat ggtaattgca tgtattcaga
    10141 tttatggaga aagtggaata gttacaaaat ctatagaaat tttactcccc tttatcaaaa
    10201 aaccttatga ttttagtgga ttaaagggaa ttttatttgt ggttgcttat actcagtatg
    10261 tatattttta tttaaatgta tatgttgcct taaaatatgt ggattactca acagttgaag
    10321 ctgcaaaagg aatgggagca tcaaagctaa gagtattttt tgatgtaata tggcctgtta
    10381 ttacacctgc tgttataact tctactatta taacttttgc atcagggatt agttctttct
    10441 cagctccaaa tcttataggt ggtggtttta aagttttaag cactcaaatt gttaggtcta
    10501 aggctaataa ccatatggat atagcttcag ttcaagtaat tactttatta ctaatgggga
    10561 ttttatttat gattttatta cagtattatg gcaaaaaata tggagtagtg ccatccttaa
    10621 aatctcaaag ttataaagtt cataatagaa aaaaagactt atttacattt gtatgtaagg
    10681 ttattattga aatacaaatt attatgatta tgcttcctat agttactatt gtgtatttat
    10741 cctttgtaag cacacattca attatgacaa ccatatttcc tagagacttt acactggaaa
    10801 actatcagat gattttccaa aaatcacgtg tattacagcc tatattaaat agtttcaaaa
    10861 tgtctttaat ggcagtagca gtaggattga taattactgt accagtatca tatttagtaa
    10921 ttaaaaagga tactgcatat aatcgactag caaaatttat tataatgtta ccttggagta
    10981 tgcctgcaag cgccattgca gttaacctaa ttaatacatt taatcaaaaa agtatatttg
    11041 cttttaatga atccttaata ggaggttttt atattgtacc tatagcatat acaatttatg
    11101 ctctaccatt attattttca tccaatgagg ttgctatgaa gtcaataaat ttaggactag
    11161 aagaatcatc aaggagttta ggtgctggaa ggataaaaac cttttataat gtaatagttc
    11221 caaatatgat gccaggaata atttcaggag ctatattagt attcattcgt acaattgggg
    11281 aatatacaat gtcagccttg ctttatggtg tttataatag acctatttca atttctattg
    11341 taactaatat gcaagaattt aagataggag tatctatggc ttatggtgta ttagtaattg
    11401 gtatatgcta tatagcatta ggaataattt ttaaattaga tagaaaaaga tttacataag
    11461 ataacaaagg ctaatttata aattggtgct tagaaaacca aatcacttta ttagctaaag
    11521 atgaatttga agggtgacac tttggtgttt tatgtaaagt ttatggaagt aaaacattaa
    11581 ataatcgtga gtatgattcc tataaatcgt atatactatt gaaatccaag aaaaggtatt
    11641 ggctaaatag ctaatatctt tttaattttt tgactcccat atagcattaa ctattacatg
    11701 agtatcttta aatttatagt taaagtatct taattgttta ttataaaaat ataggatggc
    11761 taaagcacat taaattaaaa cacttaaaaa gagaagatgt acatgagaaa tataattaac
    11821 tatgatacag acttctaaaa attatttagt gaaaaggaac ttagagtgta taataaaagt
    11881 tccttttcaa taaggaaaac gattgttatt ttctgagctt cattaccctg atattattta
    11941 aatataaaca acaacaaaat taacaagaag taaattcaac ctaaaattta aaatatcggg
    12001 aggtacaata tgttaatcaa tatgaaggaa atgctaaaag tggcacaaga aaatcaattt
    12061 gctgtaccag catttaatat aggaagtggg caaatactta aagcagtagt acagggtgct
    12121 aacgaaaaaa atgcaccagt aatacttgca atacacccaa atgagctaag ttttctaggc
    12181 gatagctttg tagttagttg catagaagaa gcaaataaga gtaaagtacc tatggtaata
    12241 cacttagatc atggggaaaa taaagaacaa atattaaggg caataagatg tggatttaca
    12301 tcagtaatga ttgatggctc acatttacca tatgaagaga atgtagctat atctagggaa
    12361 atagtagaaa tagcaaaagg attaaatgtg tccgtagaag gagaacttgg aacaatagga
    12421 acaacaggaa catcaagtga aggtggtact gatgaaataa tatatacaga ttcaaaatta
    12481 gctaaggatt ttgttgaaaa aacaggagta gacacactag ctattgcaat tggtacagca
    12541 catggtatat atccaaaagg ttttaaacca gagcttaaac tagatttatt aaaagaaata
    12601 agagaagtag tagatatacc tttagttctt catggtggtt catcaaatcc agatgaagaa
    12661 atagcacaag cagttaaact aggagtttgt aaaataaata tttcgagtga tgttaagtcg
    12721 gcatattata agaagtgcag agaactatta gaacaaaacc caagcttata tgagccagat
    12781 actatatatc caccatgtat aaaagctgca agagaagtta tagagtttaa aatgaatcta
    12841 ttcaatgcta tagataagtt aaagtgcttt tataaaaata aaaattaatt atgaggtaac
    12901 aaattaaaag gaggggagaa tttatgatag gtaaaatact acgaagcgat tgtattgtac
    12961 tagataaaca attacaaaca aaaaaagatg taattaatta tatggcaaat catttgtacc
    13021 atggaaatta tataaataat aaagaaaagt ttatagctga tattaataaa cgtgaaaaag
    13081 aatcatcaac taatatgggt aatttaatag ctatacctca tgctaagtca aatgtagtaa
    13141 atcaacctgt aatgatgtat ttaagaacag atagagagat tacagataat gatggtcaaa
    13201 aacttaggtc attttttatg atagcagttc cagaatcagg ttcagatgaa catcttaaga
    13261 ttatatctaa actagctaca tatctaatgg aagaaaattt tcttaagcat ctactaagtg
    13321 cagaaactcc atacgaaata ttaagtatat ttaataataa agctgcagaa tcaggagatt
    13381 ttagtaataa tcaaaataaa ggtcttatag ttgggataac aggatgtcca acaggtatag
    13441 ctcatacttt tatggcaaaa aaagcattag aggaagcagg gcaagaactt ggatatgaag
    13501 ttaaaataga aactcatgga gctataggtg tagaaaataa gttgactaaa gaagatataa
    13561 aaagagctga atacgtaata atagcttgtg ataaagaagt tcaactgagt agatttatag
    13621 gaaagaaagt gctacaaaag aaagtagtag atgctaagaa tgtttcatta gctaaaaatt
    13681 taattataaa cgctgaaaat ggaatggcta aagaaattaa accaggtgga aagactttag
    13741 aagattcaaa agaaatgctt caagggtcag agacaaaggg tgtttataag cacttaatga
    13801 gtggtgtatc tgaaatgatt ccatttgtag tgataggtgg aattggtata gccatagcat
    13861 ttatgtttgg tatatttgca tcagacccaa ctcatgaaac atacaaccct atagctggat
    13921 tctttggaca acttggaggc gattcagcat ttaagttatt tataccgata cttgctggtt
    13981 ttatagctaa atcaatagca ggaagacaag gatttcctcc tgctatgata gctggtttta
    14041 tggcagttat tggaggttca gggtttatag gtggtatggt agctggtttt gcaacaggat
    14101 atatagcttt aggagtaatg aagataacag aaaatttacc agatgtatta aaaggagtaa
    14161 atgctgtatt gatatgtcca cttttgattg catttacgag tggagctata atgttctttg
    14221 tagttaatac gccagcatca tggttaaatg aagctttaca atcttggtta caaagtatgt
    14281 ctggaactaa taaagtatta ttaggtgcaa tattagcagg gatgatggca tcagatatgg
    14341 gaggacctat taataaaaca gcttcagctt ttggattggc tatgttttca agtaatatat
    14401 ttgagccatc agcagcatta atggtaggag gaatggtacc tccattagcg atatcattag
    14461 ctatgttaat attcaaaaat aagtttacta aagaagaaag aaagtcatct ctaccaactg
    14521 ctataatggg ggcatctttc ataacagaag gcgcaatacc gtttgcagct gcagacccat
    14581 taagaatgat tccagcaaat ataattggtt cttgtatagg tggggcaatc tgtatgagtt
    14641 tagatataac tttaatggca ccacatgggg gaatatttgt aataccattt gcatgtagtg
    14701 caccagttgc atatatagct tgtatattga ttggtacagt aataacagca ttgattatag
    14761 gggtagcaaa gcctactata agaagtgaaa acagtgaagt tgaattactt ggtgcttaaa
    14821 ttataaataa tagtaaaaat aagtaatgga ataacaacaa gtatatagaa taataataag
    14881 gatatttaga atcagttaac tacttctaaa tatccttaat ttttattttg aaattatatt
    14941 atctattata tcaatttagt aaggtgtatc ataaattctt ttatgataca gaagtatctt
    15001 agtaatttta catagttagt aaatatgtta aaatagtact taatataaac atagaaatat
    15061 ataaacatag gtagggaggg ctaagtatgc ttgtgtcgag gcaaattgaa tttcttaaat
    15121 tgatactaaa cgaaaataaa tatagaccta taaaatactt taaagataaa ttaaatgtat
    15181 ctgataaaac tttacaaaaa gatttgaaga tgattgagaa atatttagaa acttttaata
    15241 taaaaattga tataaaaaga ggctatggaa tattaataga agatttagca aagaagaaca
    15301 tagacttaat taatagttta aatatacaat cgaaggaaaa taaatcttta tcagtagagc
    15361 agagaagaat ggagatttta aagtatttat tactaaattc aaataatata acttcaataa
    15421 atcagttagc agataaatat tatgtaagta aaacttctat agtaaatgat tttaaatata
    15481 tagaggcatg gataaaagaa tacaatttaa aacttaataa aactttagaa ggaact
//
LOCUS       DAEPOZ010000081        15498 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.081, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000081 DAEPOZ010000000
VERSION     DAEPOZ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15498
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.081"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..576)
                     /locus_tag="KR440_001848"
     CDS             complement(<1..576)
                     /locus_tag="KR440_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBH0698899.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIKDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAEDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSGLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIK"
     gene            complement(703..1392)
                     /locus_tag="KR440_001849"
     CDS             complement(703..1392)
                     /locus_tag="KR440_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH0698900.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLRVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMIGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            complement(1400..1963)
                     /locus_tag="KR440_001850"
     CDS             complement(1400..1963)
                     /locus_tag="KR440_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBH0698901.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            complement(1965..2933)
                     /locus_tag="KR440_001851"
     CDS             complement(1965..2933)
                     /locus_tag="KR440_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBH0698902.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLHNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     regulatory      complement(3034..3077)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3442..4071
                     /locus_tag="KR440_001852"
     CDS             3442..4071
                     /locus_tag="KR440_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0698903.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKAIMHSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQSY"
     gene            4046..4450
                     /locus_tag="KR440_001853"
     CDS             4046..4450
                     /locus_tag="KR440_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBH0698904.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            4453..4926
                     /locus_tag="KR440_001854"
     CDS             4453..4926
                     /locus_tag="KR440_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBH0698905.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNKIELSK
                     FDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPSS
                     LLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
     gene            5473..6138
                     /locus_tag="KR440_001855"
     CDS             5473..6138
                     /locus_tag="KR440_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBH0698906.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            6160..7092
                     /locus_tag="KR440_001856"
     CDS             6160..7092
                     /locus_tag="KR440_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBH0698907.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            7113..7754
                     /gene="pcp"
                     /locus_tag="KR440_001857"
     CDS             7113..7754
                     /gene="pcp"
                     /locus_tag="KR440_001857"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBH0698908.1"
                     /translation="MKILLTGFDPFGGELINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIK
                     GGFIHIPYTTSQVINKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(7873..8205)
                     /locus_tag="KR440_001858"
     CDS             complement(7873..8205)
                     /locus_tag="KR440_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0698909.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            8471..9091
                     /locus_tag="KR440_001859"
     CDS             8471..9091
                     /locus_tag="KR440_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH0698910.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKEAI
                     PGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            complement(9416..10240)
                     /locus_tag="KR440_001860"
     CDS             complement(9416..10240)
                     /locus_tag="KR440_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HBH0698911.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNI
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     TLFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(10251..11654)
                     /locus_tag="KR440_001861"
     CDS             complement(10251..11654)
                     /locus_tag="KR440_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0698912.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIREYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDNGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            complement(11658..12383)
                     /locus_tag="KR440_001862"
     CDS             complement(11658..12383)
                     /locus_tag="KR440_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0698913.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDVQNESKNLNKNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            complement(12495..13268)
                     /locus_tag="KR440_001863"
     CDS             complement(12495..13268)
                     /locus_tag="KR440_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0698914.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFLGFLLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNEPIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVRYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            complement(13274..14161)
                     /locus_tag="KR440_001864"
     CDS             complement(13274..14161)
                     /locus_tag="KR440_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0698915.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDANYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRVGIAQVLLNDPKIIVFDEP
                     TAGIDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDIDSVVLRIVSEDIPSGDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            complement(14175..15197)
                     /locus_tag="KR440_001865"
     CDS             complement(14175..15197)
                     /locus_tag="KR440_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698916.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFITPVLETERYYID
                     KEKGCIEDTRSGIDIGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALK
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
BASE COUNT         5573 a         2379 c         1741 g         5805 t
ORIGIN      
        1 tttaataaaa caaattgata gcaaccatgc catacccatt ttaacataat attcttcact
       61 ttgtatatta ttacagtatt caaatatttc tttaatatgt ttatcatcaa cataatatat
      121 taaaaacata actaatgcaa atctaatttc ccatggattc tttgaataaa catatttttt
      181 aagtatatca tacatatttt ctttatattt tttcatagac tttaaaccag aacaaaaccc
      241 atcgcaaact gaccaattat cgattttagg tataaatctt tttacatagt ataaagtttc
      301 ttcaaaatta ctatttatat tgccaattac cataccttgt agcctaactt cttcatagta
      361 tgtatcctct gctacttcta aaaattcctt ccaattccct tggcttattt cttttgataa
      421 ctttcttaaa ttaggaactc taatacctat tatattttct gttccaggta taagcttttt
      481 attaaaatct ctatatttta tatcttttat agacatcata tattctaaaa gttcattata
      541 atccaattta ttccattttc ccctatcaaa catcatttaa ttgctcctta attccttttt
      601 tcttattgta acataaaact ctacaaaaaa agcttctaac tattagaagc tttttcaaaa
      661 acataagaaa cttttcaatt ttaaatttaa actaaattaa tgctaatctc ctacaccaaa
      721 gtctgtatta ttgtataaaa cttctatatt tggattttca tccaagaatt tatacttact
      781 aacaaaccat ttcactaact cttcatctct ttttatacta gaagaattat ttataaaaac
      841 attcactgtc gctttttcaa aagtatttag gataatctct atatctttat ctatcatctc
      901 ttttgtttgt ccttttattc caatcataat acaaggtgaa tcaaaatatt cttttacatc
      961 ttgtggagtc ttaaaatttg catttttatt taaaatatta tttctaaaat cattgtcaaa
     1021 ggtttcaact ccaattttaa ataccacagg aatttcaaaa tactctctca tttccttaag
     1081 cctatttttg taagaccaat gactttctaa aaataatttt tttatattct tctcttttat
     1141 aatacacttt attttttcta aagtatcctt aggtaattca aaacagcttc cagaatttat
     1201 gacttcaaga actccatatt tcccagttac attttttaaa acccttagat ttaatttatt
     1261 catctcttct tctaaattag aattatcatc tatataatca caaaaagaac actttcccca
     1321 tatacaagga aatcctttaa gcagaacaat ttctctttga tttttatttg ttatctcact
     1381 atatctatcc attttctatc tattttcctc cagtcaatga tacctttaaa gtttttggaa
     1441 ctgcatttaa tcctaaattc actaaaacaa ctgctaacag cttatctcca taatcagtaa
     1501 atacttgggt tacaaacaca ctaaaaacat ctcccagtcc taacactttc aagatttgca
     1561 ctatatagga tgaacctgaa gaggtcatac ctccaaataa aaatgctgaa attattgaac
     1621 ttattataga tgttggaata gcaaataaaa gtactcctag tggagttttt aaacccctta
     1681 aaaaattctt tttaaatgct attccagcaa gtaatccggt tgatatttga actggtgcaa
     1741 aatagagaga atacacatca aacgtaattc cacttattaa acttccaaac acacctgtaa
     1801 caacagcgta tttaggtcct aaaagtgaag ctatcattat agttcctatt gaatctaagt
     1861 atacaggtaa tcttaatccc atagctataa aagcgcctat tatatttata gaaataccaa
     1921 aagctataag tgtaatagtt tttacacata tttttctttt cacactatat cactccttcc
     1981 acagagtcta taatgtcttc ataaccttta aaaagcctct ttaaaaacat gtacataaat
     2041 ttcttctcat caacttcatt tagaacaatt gcattaggat tttttttata gaaattgaaa
     2101 gaatccacta tagactgacc catagctatt ccatcttcta caacttctac atatgattca
     2161 aatcctttac atatacttct gtctataaag tacgctactg ctagagggtc atttatcaca
     2221 cagccaatta ttccttcctg ttcccaatgg aaatcaatat aaaatctagt tatttcagtt
     2281 atatactttg ccatcttctt atcaagtcta tttataaact caataatatt aggagtaagt
     2341 acaatttttc tagttacatc taaacctacc atgtggattt ttttagataa attcttgtaa
     2401 acataatctg ctccatgtgg gtctacccaa taattaaact ctgctactgg agagcaattt
     2461 ccatgaatcc taaatgctcc tcccatagat acaaactcat cgagattttc aaatgctttc
     2521 ttatctttaa taagtgcttt agctatattt gtaagtggtg caagtgctat tattgatact
     2581 ttttcatgat tatgtaaagt atttattata aaatccactg ctccattttt tgctttagct
     2641 cctacaacct tttgataaaa gttttctcca ataccatctt ctccatgtgt atcttgagct
     2701 gttacaagtt ttctttttag tggtgcttct tctcctatat atactggaat atttagtgaa
     2761 gaacacattt gaagtgtttt tagtgcattt tctgcaccta tatttgctgg aacatttcca
     2821 caacatgtgg taattccaat tacttctagc tctggtgagt ttaaagccag aagaattgcc
     2881 aaagaatcat caattcctgg gtcacaatca attattactt ttctcttttc cattttttat
     2941 cctccttgat ttgtggtcga ggagaactac acatcttttt tccactttca aaaaggtgtg
     3001 tctaagggta ttaattatat actgccacta tataattcct tagtttttta tacttggagt
     3061 tctcgaacaa gtacagatgt aaaatcatat ttttaggcta cttactatag actcttaaaa
     3121 actatagact gttaaaaaat ttttaaacat ctgatttaat agctataaaa taaattttat
     3181 agcataaaag aatttattat attttaatgt atcttattaa agtttattta gaattatgaa
     3241 ccaactttaa taagatgtgg cgcatttaaa ccgattatta ttatacaata atatcttaaa
     3301 tattacaatt aataacatta atttattatc cataaaaata tattaactta tattatttaa
     3361 taatttattg acagtattaa aattaaatta tacaataaca gtgtaaatta acttaacacc
     3421 gttaagcaaa gaggtgatta tatgagtatc aaagaaagac gtaaaaatga aaaagaagaa
     3481 atgaagaaaa agattatgga tgctagcatc gaaattataa accaacatgg atatgaaaat
     3541 ttatcaataa gaaaaattgc tacaaaaata gaatattctc caactacaat ttatctttac
     3601 tacaaggaca aagcagagat tatcagcgat atgaccaata aactttataa cacagtagaa
     3661 agtaatgcta ttgacattat gaataattgc tcatcactac caatagataa acaaatcata
     3721 gaaattatga ctagctttat aaaaactctt tctagtgaac cagagatggc aaaagctatc
     3781 atgcatagta gaatgaatat tatatttgca tctgaaaata caaacaatac tcctagcaat
     3841 aatggtataa aaatgcttga taaattcctt tccattggaa ttgaacaagg aatattcaaa
     3901 aaaaatatag ataaaagctc atggatgctt ataagtgcat tgttaggatt cgttttatgt
     3961 gttgttgaaa ataaactata ttctttacat aatttttctc aacttataga aaatttctta
     4021 gatattttag tgggaggact ttacaatgaa aattcgcaac aaagctacta ataaaaagaa
     4081 aaaatatgta aaaatatcta ttgctctatt ctttgtattt atatgcatta ttattgtaat
     4141 tgtaagcaac ttaaagccag aaaacttggt agtaaaagat atagatatta ataatgttaa
     4201 aaatggcatt tatactggaa gtgctgataa taacttggta aaagctactg tgtctgttga
     4261 agttaataat ggtaaaatcc aaaatattaa tatcttaaaa cacgaccatc ttttaggtaa
     4321 acctgctgaa aaaattacaa caagtattat taaacaacag tctttagatg tggatgctat
     4381 aacttctgca acttatagca gtaatactat acgaaaagct gtagaaaatg ctttaagaaa
     4441 gggagaataa ttatgaatac tattattata tattcaagta aatatggttg tacaaaagac
     4501 tgtgcaaata tactaaaaaa taaactatca gataatgtca ctattgttga tattaataat
     4561 aataataaaa ttgagttatc aaaatttgat aaaattatta ttggaagttc tatatatgtt
     4621 ggttcagttt caaaaaaaat acaagtgcta tgtaatgata atgtagagtt attaaacaaa
     4681 aaacaagttg gcattttcct ttgctgtggc ttttctgaac aagcagataa atatttaaaa
     4741 tcaaattttc catcatcctt attagaaagt gcaaatgcta ttggaatttt tggtagtgaa
     4801 gctcgattag aaaaaatgaa atttttagat aaacttatta tgaaggctgt ttcaaaaggt
     4861 aattatgaca gttttagaat atcacaggat aatatagata attttttaat aaatcttaat
     4921 agctaaaata aaactaatta tttctatgat aaattctttt atttataagt aatattgctc
     4981 aaaatagtct ttaaacttta tcaaaatttt atttttctta gtgaaatcta atgaaactag
     5041 ctcttaatat atgctttatt aatcataaca aaaagagagt gtctaatgaa ctaaaaagtt
     5101 cattagacac tctctttttg tatgaaatca aatatatttt tatcatttca aaagagaaaa
     5161 atacacttgt ctctatgttt gacacattct ctttttataa tataatatct aatttaaaac
     5221 ctatttcaaa tattgacata tacttaaacc atagacttac aaaatcaatt catattagct
     5281 taaaatataa tctttgttac tcaaattcct atacacaacc attatttact ttgatacttt
     5341 aaaacatctt ccctattttt tattaaattt tataataata gtcctattaa cgcaaatact
     5401 ataaaaagag tttaacttta tccattaaat aaatttaaat taaaacatat agaaatggaa
     5461 ggagaacaat ttatgataaa actaatcggt atattgatag ttgtaattgg atttatactt
     5521 aaaattgaca cattatttac tgtattatta gcaggagtgg caacaggaat tgttgcagga
     5581 ttagatttta atcaaatttt tacaatttta ggagattctt ttgtctcaaa cagaggtgta
     5641 tcattattca tattaacact gccagtaatc ggtgtattag aaagatatgg tttaaaacaa
     5701 agagctgttt ctttaataga aaaattaaaa agacttacaa caggaaaagt acttactata
     5761 tacatgatag ctagacaaat agctggtgct ctatcaataa gaatgagtgg acatcctcaa
     5821 tttgtaagac cacttgttaa tcctatggca caagctgctg gtttaagtaa tagtgatgaa
     5881 ctaaaagaat cggatgaaga agccataaag gcattatctg ctgcatcaga aaactatgga
     5941 aatttttatg gtcaaaactt atttgcaggt agtagtggag tattacttat agcatctact
     6001 ctaactcaat ttggatacaa tgtaactgga cttaatattg taaaggctag tactattatg
     6061 gcagtaatag cacttgtagt ggctacaatc cagtttacct tatatgataa aaaattaaac
     6121 aaaagtcata aaaaatagtt ttatttagga ggaataaaaa tgcaaaattt tataaacata
     6181 tcattggaaa tcttttacgc actaatggga tttttaatga ttgttatagc atataagtct
     6241 tttactacca taaataacaa taagaaatat ggtacatcat tattttggat acttatatca
     6301 ctgccattca tatttggtag attgattcca gccaatataa taggaataat attaatatta
     6361 tctagtttat taacactttc taaacaagtt gtatttgcaa agtatgaaga accagatgaa
     6421 aattttggaa aagaacaagc agacaaactt aaaaataaaa tatttatacc atcattaata
     6481 ttagcatttg ctgcagtagt agtagctatg agcctttcta attttgataa ttcttctcaa
     6541 tttgcaatag gtgtgggttc aataattgca ttaatatcag cccttataat aactaaagca
     6601 aaacctgcta catcagttca agatggctca agattacttc aacaaatggg acctgctagt
     6661 atgttaccac aacttttagt tgcattagga gctttattta ctcaggctgg agtcggcgaa
     6721 gttatttcaa ccatgatatc tggtgtagtc cctgctgata gcagattatt tggtgttata
     6781 gcttatgtat taggtatggt tatatttacc atgataatgg gaaatgcatt tgccgcattt
     6841 tcagtcataa cggcaggaat tggtattcct tttgtattgt ctcaaggtgg aaatcctgct
     6901 ataattggag cattagcatt aacagcagga tattgtggaa ctctacttac tccaatggct
     6961 gctaatttta atatagttcc agcagccttg ctagaatgta aaaatgatta tattgttata
     7021 aaatatcaag ctccagttgc tttggtatta attatagccc acatattagt aatgtacttt
     7081 ttaggatttt aatataatta ggaggtaaaa taatgaaaat tttgttaaca ggatttgacc
     7141 catttggggg agaactaata aacccagctc aagaagcagt tgaaagggtc aataataata
     7201 taaacggtgc agaaatcata aagataacta taccaacagt tatgacaaaa tctgttgaag
     7261 ctatagataa agctattcaa gagcacaatc cagatatagt aatatcagtt ggacaagctg
     7321 gaggtagatt tgacattaca cctgagagag ttgctataaa tatagatgat tttagaataa
     7381 aagataatga aggtaaccag gttatagata ctattataaa agaagatggt gaacctgcat
     7441 atttttcaaa attacctgta aaagccatgg taaaacatat gaatgaaaat aaaattccag
     7501 ctagtgtttc aaacactgca ggaacattcg tttgtaatca tgtgatgtat ggaatccttt
     7561 atatgataga caagaaatat cctaatataa agggtggatt tatacacatc ccatatacaa
     7621 cttcacaggt aataaataaa aaaaatacac cattcatgtc tttagaagaa atagtaaaag
     7681 gtttagaatt agctatagaa gcttgtatta tatacaaaga agatgtaaaa gaaattggtg
     7741 gtgaaatttc ataataaatt ttttagtatt ttataaatta aaaatactaa ctattaattt
     7801 aaatatagac tgtgagtaga ttattcaaac aattttactc acagtctttt tatggcatct
     7861 acagatacaa aattaaggtc tattcctctc tccccactca cacattgcat ctaaaattgg
     7921 aatcagagat tttccccttt ctgataaact atattctacc ttaggaggta tttgaggata
     7981 ttcagtacga ataatcaagt tatctgcttc taattctttt aatgataaac tcaaagtttt
     8041 ataggaaata gtatttatat aacgttttaa tgcattatat ctgacaactt caaattctac
     8101 aagacagtaa agaattatca ttttatattt accactaatc agtgaaagtg tataactaaa
     8161 tcctgtttca cttaaatctg tatttgatat acagtctata ctcatatatt acactctcct
     8221 tttggttagt attgaacaaa aatgtgcgta cttgttttta gaataattta taataaaatt
     8281 atagtgtaag aataattgaa agtcaatttt ataataggag gtgtatttac taaaaaaaga
     8341 gttattttaa ataggattct aagattagac atttctatat aaattgataa tattcatgaa
     8401 aatcctattt tataaaaaat aatatctctt agaaaatcta ttaaataaaa ttcattggag
     8461 gaaatttata atgaaagttt tattgataaa cggtagccca aaccaatatg gatgtactta
     8521 tacagcacta aatgagatta ctaaagtatt gagtaaacac gatattaaaa cagaaatact
     8581 gtatttaggt aaagaagcaa tcccaggatg tattagttgc gcatcctgtt ttgaaactgg
     8641 aaaatgtatt cgaaatgata aagttaatga attgattgaa gatctcgata atattgatgg
     8701 tattattatt ggttctcctg tttacttttc tagtgcaact ggtcaattaa cttcttttct
     8761 tgaccgtcta tttttcatag ctggaagtcg catggctaca aaattaggtg cttcagttgt
     8821 gtcatgtaga cgtggtggtg caagtgcaac atttgaccaa ctaaataaat atttttcaat
     8881 cagcaatatg cctattgtat catcacagta ttggaatcag gtacatggct ttactccaga
     8941 ggatgttatg aaagatgaag aaggattaca gactatgcga actctaggag aaaacatggc
     9001 atggcttctt aagtgtatat cagcaggtaa aaaagctgga attaaagaac cacaatatga
     9061 agaaacaatt atgactaatt ttataaaata aataaaaaat gcactaatat taattaaaac
     9121 aaaacactaa tttataaata aaataagtat aaatatagga taaaaaacac taaaacagag
     9181 gtattgaatt ttatatcaaa cctctatttt attattacat taataaccaa accatatata
     9241 acatcatata tttctaaata aattataata ttatattcta atgttattga aattacaata
     9301 actgaatttc tgctcataaa tttaaatatt ctacaaatct catctaaata acaattttaa
     9361 tagacctaaa acaatcctca tattgttcat tttgtattaa aaatatcact tacaatcaaa
     9421 aaccatttat taagtatatt cctaagatat acaatatata tacatggatt ggatagtata
     9481 aataaaataa atacttcata ctcttccctt tttctccatt gtaaagtaac attaaaggta
     9541 gagaagcaat catcatccat tggtaatttt gtaaaataga attttttaaa ctataatctc
     9601 catttgacat tgttgtaaat ataaatatac acatcaaaac ataacaaata ctcaaactga
     9661 actttttacc tctacacaaa taaaaaataa tcccaagtat aatccaaatt ggtccacctt
     9721 ctactaaaaa tggtactggt accaaaatca taaataaagt aataactctg ttagtgactt
     9781 taaaaagtaa agctactgta aatattccta aaattatagg aatgattatc atcaataatc
     9841 ctataataaa atatttaatt tctttctctt ttctaaattt ctttatgaac tctattgctt
     9901 gtaaataaat cgctattaaa aataatgttg aaaaaatgtt atttataatt atagctcctt
     9961 ctggatgtga aaatacatta ttaattattt gatttcctat agccataata acagaccaaa
    10021 tatacaatct acttatatat ttttttctgt tactagtgtg ataaaatcct tcaacagcca
    10081 tgaatataaa cagtggtgct gatattctgc ctacccaatg aaaccaaatt ggtatttgca
    10141 ttgtttgtgg cataaatgct gctatatggt caaatgtcat aaatattaat gctaaaatct
    10201 taatagtaaa cccacttata ccttttttaa aaaaatccat atcatatctc ctaattttgt
    10261 tttatctcta taataatttt tgttcctatt ccaatttcac tacttacata aattttacct
    10321 ttatgaatct ctattatttc tttagaaata gccataccaa gacctgaacc tttatgcatc
    10381 tcacctgtat tagtacctct ataatatttt ttaaaaatat gtttcaaatc acttttgctt
    10441 attccaattc cattatcttt tattataatt tctatattat ctttttttac aatttctaca
    10501 cttatcaaag ttccttcgct attatgaact attgaattaa atataagatt ggttatagcc
    10561 cttctgaata agattgaatc tacatatact tcaattaaat cttcattact ttcaaattca
    10621 atattcctat ttttatatct attatcattt aaaatatcaa ttattatatc tctgactaaa
    10681 ctgactaaat ttattttttt cttgtccaga actatagtat tattttttaa tcgagtagat
    10741 aaatttaaat catcaactaa atcttttata tacttagact tattatatat aatttctgta
    10801 tactctcgta tttcatcttc ttcaaactca taatctttgt cattaattat ctctgcatac
    10861 ccttgtatag atgctagagg tgtctttata tcatgtgtta tatttgccag ccattcctct
    10921 ctcaactctt cattttcttt tctctcaact tcatttactc ttaaagtatc agctaacata
    10981 tttatatttt taaaaacttc ttcatatata cctctgtcct taagatataa actataattt
    11041 ccattagcca tagtctcaat actccatagt acgcttgaaa ttggttttgt aagttttcta
    11101 ctaaataaat atcctacacc taaagctacc acgctatcaa ttactagtgt tataatgata
    11161 actttattta aagttttttt aatgttattt tgagaatagg ttaaaattat tcttgatata
    11221 tctctactag gtatacctac tattatgttt agatgttttt tgtttaaata tttttctagt
    11281 acaaaattaa catactttgt ttctatatac ttgtaattat tcaccatttg aaagggtgta
    11341 tatttttttg gagtatcctt tggcacattt actccataaa cttcctctcc taaatcattt
    11401 aaaacctgta tccaggaatt tgatttttct aatttttcag ctccaatctt agatagttta
    11461 aattctccat ctttttcata taaatctttt tcaaaagaac gtgcaaattc ttcaggatta
    11521 tttccagaat ctacaacttt aaaaaagcta ttagttgaaa tcacatacaa tatagatata
    11581 atatttataa ttactactga tatagctaca aacacaattg taaatataaa atttctagta
    11641 atcttccact tcatattcta atcttccttt actgctaatt tatatccaag accttttatt
    11701 gtcagaatat attcaggctt tgaaggatta tcctctaatt tttccctaat ttttcttata
    11761 tgaaccatta tagtattgtc aaatccaaca taatcttctc cccacacttt atcccatagt
    11821 ttttctttac ttataatttg attttgatta tgtgcaaata aagataggat ttttagttcc
    11881 tttgcagaca gtcctataga ttttccatct tttataagtt ctgccctact ttcatttaat
    11941 atatatgggc caaatgatat aattttttct tcattcttat ttagattttt actttcgttt
    12001 tgaacatcac taaaataact taatcttctc aaatgcgctt taactctaaa agccacttcc
    12061 ttagggctaa aaggttttgt aacataatca tctcctccaa tcgctagtcc aagtaactta
    12121 tctaattctt cggttttagc tgataaaaat attattggag aattggaggt ttttcttatt
    12181 tctttacaaa tatcataacc ttcgccatct ggtaacataa tatccaatac aatcaaatca
    12241 ggattaatac ttttaaataa ttctaatcct tcttttttag tttttgcagt atatatatta
    12301 ttaaggccct ctttttttag gacagtttct aaaagcttca atatttctac ttcatcatct
    12361 attattaaaa cacgattttc cataaacaac ctcctgtaat aatttacata tatattagca
    12421 tatattttat ctaaaattca tttatcttat ttttaattaa actaaaatat aaataaaatt
    12481 actattattt ttagttaaaa ccttttgctt gatgtaaaat tttcatatgt tattttagtt
    12541 aacactatca ttaatataat tgagattact atagaaaaaa ctatcaaaat acttggtaca
    12601 ttatacctaa ccatttgttg gctcatcata atccaatttg gagtcactga cataaatttt
    12661 atagctttat ctggtataaa acctacacta aagtatggaa ttgccattat caatccacct
    12721 agtataaatg gaattacagc cgatttaaat atagatgaaa gtgttaagcc tatttgtgca
    12781 aatataaaag aagcaaaaat agtagtcaat ataacaatta tatacatttg tattactgta
    12841 agatttaaat tactatttcc aaagtaccat aagttatgaa tcggttcatt taaattttta
    12901 aaatcaaaaa aactaaaacc tctaattcct actaacaata taaaagaaat attaataata
    12961 gataaaatta atccattagc tactagtttt gataatacta ctgatttttt tcccttttta
    13021 gttgtattta ttaaacctgt catattagaa tcatattctt taatgtaatt tccagaagtt
    13081 attataaaca ttatcaaacc tattagaggt attgagcctt ctatcttgta aaatatatct
    13141 gcataagtat ctaaaacatc aaactttcta atcagaagaa atcccaaaaa tattaaaaac
    13201 agccaaatta tccaaacact ctttgatttt aatattttta tcatttcagc tttaactaaa
    13261 tacatcattt ctactacacc tcccttgcat cttccaaatc aaaataaaac atgtatacat
    13321 cttcgaattt tgcttgaact ggttgtatat tatccccact tggaatatcc tctgaaacaa
    13381 ttctaagtac tactgaatca atatcacttt gaatacttac aaccttatat ttagattgta
    13441 tatcatcaac ttcacattca tttttaactt gaacattata tactttattt tccatgtgac
    13501 ttaatatgtc tttgtgtgtt cctttcatta gaagtttgcc atctttaatc attattgttt
    13561 cctttgcaac tgattctata tcggaaatta tatgagtaga aagtattatt atcttatctc
    13621 tagacatttt tgctattaaa ttcctaaatc tagctctttc ttgtgggtca atacctgctg
    13681 ttggttcatc aaagactatt atcttagggt cgtttagtaa aacttgtgct atcccaactc
    13741 tcctcttcat acctcctgaa aattttccta tagacttatt tctcacatcg taaagaccta
    13801 ctaaatctaa taattctcct atcttactat ttaaatattt tccccttaaa ccttttaaag
    13861 ctccaatata ctgtaaaaat tcttttgctg taaaattttt ataaactcca aattcttgtg
    13921 gcaaatatcc taaaatattt ctataattag catccatttt aaatatatcc tcaccattat
    13981 aaagtatctg accttcatca ggttttatca acgttgttat ttgtttcatt aatgtagatt
    14041 ttcctgctcc atttggtcct agaagaccat aaactccatt ttccagagtt actgtaatct
    14101 tgtcgtttgc tactttttta ccatatatct ttgaaagatt ttctatttct aatttattca
    14161 tcttaactcc ccttttatct acttatataa tctgatttta ttactaaaaa acataatgac
    14221 atagcaccta tcaatattgc catcaaaagt cctattgcta tattgacaat gtctatattc
    14281 actttaaata tcataatatc acctagataa tttcctatta ttttattaaa accaaatatc
    14341 aaatcataat aatttacatt tgatacaact gaaagtaatt ttttaggtaa tatatggatt
    14401 accgacatta tcttagttat agatataata aatataaaaa aacttattga agatattgaa
    14461 gatttactta taaaagaccc aaatatggct atcatggccc atccttcaaa catcaaaagc
    14521 tctatgccta tatttaatat tacatacttt gttatactta aattagtttt caatgcaact
    14581 acattgtcta ctattctaaa agattctaag tctccattaa caggtactcc ttgttgcata
    14641 caagttataa taaatattcc cattaaataa acagaataaa ttactaatgg aactaataaa
    14701 gcaacaaata ttttagactt taatgctttt ttcttctctt ttgatgatag tatgatatct
    14761 tttaccgaag taacgacttc atctacgtaa atattggata taattataga tataatgatt
    14821 aacattccta catttataag tgaatttgta actctatagt taaatacttt ccaaaaacta
    14881 atatccttat attcattcac acttagatta tcaagttttt tctttgacat taattcaatt
    14941 tttttagaaa attcacctgt atctgtttga tttgaaaact gctttaaaac attaatctta
    15001 ttttgaaatt tctcattacc tatgtcgata ccacttctcg tatcttctat acatcctttt
    15061 tctttatcta tataataacg ttctgtttct aaaacaggcg taataaagat actaatcatt
    15121 agtattaaca ctaatatccc tagagatata aacatactct tagatttagc caattttttt
    15181 atttcccaat ttaccaatat atttctcctc ctaaatcatt ttaattacac atatatatta
    15241 aaacttaaat cttatataaa tataatacta accttaaata aagcttaaat tattttataa
    15301 tttataggtg tgtttattat tggagttaca tttattattt ttctgggaaa ttatacattt
    15361 tcaattattt ttattatttt tgatattttt attgattgga aattttattg ttgttcaaat
    15421 tgtagttatt ctttaaattt aaagatggag ttttttaaga atagaaccta ctgtccttat
    15481 tatggaaaga ttattaaa
//
LOCUS       DAEPOZ010000082        15495 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.082, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000082 DAEPOZ010000000
VERSION     DAEPOZ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15495
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.082"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..318)
                     /locus_tag="KR440_001866"
     CDS             complement(<1..318)
                     /locus_tag="KR440_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698917.1"
                     /translation="MLLILDNLNLNLDFDYRIIREENDDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRINKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVI"
     gene            complement(345..1016)
                     /locus_tag="KR440_001867"
     CDS             complement(345..1016)
                     /locus_tag="KR440_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0698918.1"
                     /translation="MPILEILNLGKIYGKKETAVHALKDANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSSGTIKVDGKDICCLSDKKLAKYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKVDEEYIEDIINLLGLSERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            complement(1028..3613)
                     /locus_tag="KR440_001868"
     CDS             complement(1028..3613)
                     /locus_tag="KR440_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBH0698919.1"
                     /translation="MKDNLKIALRYIISYKARSLAIALSIILATSLIVGIGTLSRSAQ
                     QAEVDKLKRETGSDHVYFKDINKNQLEYIKSRKDIKNLGITSHYGYTDPNERLAINLE
                     YANKNYLTNQSKLIKGHLPKASNEVVVEKWILNSLGLKPEINQNITFKLYQKEKPETF
                     KVVGILEDRPIEKNKGTCEIFLNLNESKLDKFSYAYIEFNNGIDINTSIDNIVKNTML
                     DENSVGKNKMLIESTRENGTLDNSSKYTAITMSLFSGIVIYSIYVISIYQRIQEYGIL
                     RAIGSTNFKIFKFMFYELFILALIAIPIGICTGIGGAQIFNRTAGNIQFEGNVTVTPF
                     VIPDKIILLSIGSIILTILIISFFTYLKIRRISPIDSIRKTFGTDKNINKVNSLISKL
                     TLNISVTKSISAKNIFRNKKGFIIIILSMSLGGIMIIKENYKYSFSDIQNKNGQEETY
                     MNADFILTNFFLKLNQSNKADSFKDIKGLNDNQIDKIKNINGIDKVKTASILDTRIEV
                     DKLNGLDYYNIINSTPYYKDFPLFIKNKNTGKYTMKQKLRGYNDEMINSLEKYLVSGS
                     INLERMKKENLAILYVPQVSKTNKYKLSYTPGTETPAVDIKVGDTIKVKYPKGEIDQD
                     LYIKLKDNYEYLEYEFKVGAIVSYPFADNYLYSGDQGIDVITSNDYLKKLTGVDKYNV
                     VYVDINKNANVKKINTLLGEIGSESPGTTTANMLMEKENFDKMTARALTYAYGIVAVM
                     FIISVFNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGILSSVITIVV
                     GLIIQFRMYYTYNFVSYGLGFSIDYKIYILVVLANIIVGILATYIPLRKINKISIVEA
                     INITE"
     gene            3821..4756
                     /locus_tag="KR440_001869"
     CDS             3821..4756
                     /locus_tag="KR440_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBH0698920.1"
                     /translation="MENNFIFPIVILTLFIFSNLIMNKYRFYKELDILKRGILTFFVS
                     CFVWILFMEGIEYIQLSLGYNFLLEGVLNIILFLILIILSFYIGFKFIRLLDNNNKQE
                     YKLQKLDSKINHKDNIIVKLRSQKHDYLKHLQVVYSLLNTGLAEDAKEYITNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKNISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSAIQKEKKYIRLHAYEDEFQYIIAIKNNGPKIHNVESIFLDGFSTKEGQDRGF
                     GLYLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            4775..5512
                     /locus_tag="KR440_001870"
     CDS             4775..5512
                     /locus_tag="KR440_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0698921.1"
                     /translation="MIKVLICDDDKIVRDDIANIVKESGYVDYVKKVKDGIEAIEFIK
                     SEKIDLLIIDIDMPYKNGIDSAKEILSIDKDIHIVFVTGFADYSLESFKVHPIDFIVK
                     PFKKEKILDSINIAIDHINSHKIAKNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLYTRDEDTIKFYENFDDLKKRLPPNFFMTHRQYIVNLRLIHKVIPINKSSLEIKF
                     INFQESATLNKSLEKDFLYRFYRVKRF"
     gene            complement(5672..10729)
                     /locus_tag="KR440_001871"
     CDS             complement(5672..10729)
                     /locus_tag="KR440_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HBH0698922.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVTSALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNSDRQELYNKLILLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTSEDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKFKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSHLANNIQYFEGIDYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDESGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQLVQPFKQVFREYYRINSDEISEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNINFEGSHV
                     LITGNLGEYSVHMGSGIVHKKGMGALNILPVHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDNKIKDPSILSNL"
     gene            complement(10754..11173)
                     /locus_tag="KR440_001872"
     CDS             complement(10754..11173)
                     /locus_tag="KR440_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698923.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSPINLKSGDIFTFIRLTLLAGRMF"
     gene            complement(11406..11657)
                     /locus_tag="KR440_001873"
     CDS             complement(11406..11657)
                     /locus_tag="KR440_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698924.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGTKFINKKR"
     gene            11746..12477
                     /locus_tag="KR440_001874"
     CDS             11746..12477
                     /locus_tag="KR440_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBH0698925.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            12536..13447
                     /locus_tag="KR440_001875"
     CDS             12536..13447
                     /locus_tag="KR440_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0698926.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEVGKIELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            13498..14310
                     /locus_tag="KR440_001876"
     CDS             13498..14310
                     /locus_tag="KR440_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBH0698927.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            14420..15112
                     /locus_tag="KR440_001877"
     CDS             14420..15112
                     /locus_tag="KR440_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBH0698928.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            15199..>15495
                     /locus_tag="KR440_001878"
     CDS             15199..>15495
                     /locus_tag="KR440_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698929.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDNKKNEEISNN"
BASE COUNT         5506 a         2046 c         2163 g         5780 t
ORIGIN      
        1 aattactttc tcattttgat tttttatatt tattatagag tcagtataat ccacttcaaa
       61 gttggcaaaa gcagagaata aatctatatc tcttaacaaa tgaattgtca tacattgatt
      121 gttcttattt atccttagaa tcaatgctct tacaaatgga aattgtattc tagaattaaa
      181 taagttagaa ccatcagtat ctatatctaa gctacgataa ttaatatcta taaatatatc
      241 tacatcatca ttttcttctc ttattattct gtagtcaaaa tctaagttta aatttaaatt
      301 atctaaaatc aataacattt aaatcctctt ttctataaat agttttagag tgttaattta
      361 agctctccat ctattataga tataattcta tctgcttttc tagcaatatt ttcattatgt
      421 gttattatta taagtgtttg gttatatttt tttatagaac tttttagtaa atccatgact
      481 tcttccgtag ttttactatc caaatttcct gttggttcat ctgctaggat tatacttggt
      541 tttgcagata aagcgcgtgc aatagcaacc ctttgttgtt gaccaccaga taattgattt
      601 ggaagatgat ttttcctctc actaagaccc aataaattta taatgtcttc gatgtattct
      661 tcatcaactt ttttattgtc taactttagt ggtaattcta tattttcttt tacatttaat
      721 actggaatta ggttatactg ttgaaaaaca aagcctactt tttgtcttct atattttgct
      781 aattttttat ctgaaaggca acatatatct ttaccatcga cttttatagt tccactactt
      841 ggtctctcaa gacctcctac taaatgtaga aatgtgcttt ttccacttcc acttgggcca
      901 attatcgcaa caaattcacc cttatttatt tttaaatttg catcctttag tgcatgtaca
      961 gctgtttcct tttttccata aatcttacct aagttaagaa tctctaatat aggcataatt
     1021 aatcctccta ttctgtaatg tttatagctt caactatact aattttattt attttcctta
     1081 atggaatata tgtagctaga atacctacaa ttatgtttgc taaaacaact aggatatata
     1141 ttttgtaatc tattgaaaat cccaatccat atgatacaaa attatacgtg taatacattc
     1201 taaactgtat tataagccct accacaatgg taattacact tgagagtatt ccatataata
     1261 ttccctcata gagtatcata tttttaaatc ctctctcaga gataccaata gctcttaaca
     1321 ttccaaattc gctagttctt gaagttaagt tataacttat gttatttatt atattaaaca
     1381 cactaattat aaacattact gctactattc cataagcata agtcagagcc ctagctgtca
     1441 tcttatcaaa gttttctttt tccattagca tattagctgt tgtagttcca ggagattcac
     1501 ttcctatttc acctaagagt gtatttatct tttttacatt tgcattttta ttaatatcta
     1561 cataaactac attatattta tctacaccag ttagtttttt tagatagtcg ttacttgtta
     1621 ttacatctat accctgatct ccagaatata ggtaattatc tgcaaaagga taacttacaa
     1681 tagctccaac tttaaactca tattctagat attcataatt atcttttagt tttatataaa
     1741 gatcttgatc tatctcacct tttggatatt tgacctttat agtatctcct acttttatat
     1801 ctacagcagg cgtttctgta ccaggagtat aacttagctt gtatttattt gttttagaaa
     1861 cttgtggtac gtataatata gcgaggtttt cttttttcat tctttccaga tttatactcc
     1921 cactcactaa gtatttttct aatgaattta tcatctcatc attgtaacct cttaactttt
     1981 gtttcattgt atattttcca gtattcttat tttttatgaa tagaggaaaa tccttataat
     2041 aaggcgttga attaataata ttgtagtaat ctaatccatt taatttatct acttctattc
     2101 ttgtatctaa gatgctggca gttttgactt tgtcaattcc atttatattt tttattttat
     2161 ctatctgatt atcattaagt ccttttatat ccttgaaaga atcagcttta ttagattgat
     2221 ttagttttaa gaaaaaattt gttagaataa aatctgcatt catatatgtt tcttcttgac
     2281 catttttatt ttgtatatct gaaaaagaat atttataatt ctcctttatg atcattatac
     2341 ctcctagact catggataat ataattataa tgaagccctt tttattccta aagatatttt
     2401 tagcagatat agattttgtt actgatatat ttaaagttaa ttttgatatc aaagaattga
     2461 ccttatttat atttttatca gttccaaagg tttttcttat agagtcaata ggagaaattc
     2521 ttcttatctt taaatatgta aagaaactta taattagtat tgtcagtatt atacttccaa
     2581 ttgataaaag tattatttta tctggaataa caaaaggggt aactgttaca ttaccttcaa
     2641 attgaatatt tcctgcagtc ctattaaata tttgtgcacc accaatacct gtgcatatac
     2701 cgataggtat tgctatcaaa gcaagtatga aaagttcata aaacataaac ttgaaaatct
     2761 tgaagttagt ggatccaata gctcttaaga taccatattc ctgtattctt tgatatatcg
     2821 atataacata gatactatat attacaatac cagaaaatag gctcatagta attgcagtgt
     2881 atttacttga gttatctaaa gtaccatttt ccctagtaga ttctataagc attttgtttt
     2941 ttccaacact attctcatcc aacatagtat tttttactat attatctatg cttgtattta
     3001 tatcaatgcc attattaaat tcaatataag cataagaaaa tttatccaat tttgattcat
     3061 tgagatttaa aaaaatttca catgtccctt tatttttctc aataggtcta tcttcaagta
     3121 ttcctacaac tttaaatgtc tctggttttt ctttttgata taatttaaaa gtaatgttct
     3181 gatttatttc tggttttaaa cccagactat ttaaaatcca tttctctacg accacctcat
     3241 tactagcttt tggaaggtga ccttttataa gtttggattg attagtcaag taatttttat
     3301 ttgcatattc taggtttata gctaatcttt cattaggatc tgtatagcca tagtgagatg
     3361 ttatacctaa atttttaata tcttttctag actttatgta ttctaattga tttttattta
     3421 tatctttaaa gtatacatga tcacttccag tttctctttt taatttatct acctctgctt
     3481 gttgagcact tcttgagagg gtaccaatac caacaataag tgatgtagca agaattatac
     3541 ttagagcaat tgctaaagac cttgctttat aacttattat gtatcttagt gctattttta
     3601 aattatcttt catatttacc acttcctttc ttagctaaat caattatatg tttatttaaa
     3661 atatatatca aagtaagtat cttcaagttt tgagtactta attgtacaaa tttatatgtg
     3721 tatttgcaag tttaattaat gtacatgtaa attttgactt tttacataat ttttctaaaa
     3781 tgatataatc ctcatataaa gtgtagatgg gaggggaatt atggagaata attttatatt
     3841 tcctatagta atactaactt tgtttatatt tagtaatctt ataatgaata aatacaggtt
     3901 ttataaagaa ttagacatat taaaaagagg aatattaacg ttttttgtat cgtgttttgt
     3961 atggatttta ttcatggagg gtattgagta tatacaatta agtttaggat ataatttttt
     4021 acttgaagga gtattaaata ttatactttt tttaatttta attattttaa gcttttatat
     4081 aggatttaaa tttataagat tactagacaa taacaataaa caagagtata agctacaaaa
     4141 attagacagt aaaataaatc ataaggacaa tataatcgta aaattaagaa gtcaaaaaca
     4201 tgactattta aaacatttac aagtagtata ttctctatta aatactgggt tagctgaaga
     4261 tgcaaaagaa tatataacga atatatcaaa tagctttaaa tcaaaaaatt caagatatgg
     4321 gaaagtatct tatttagatg ctatagcttc cttaaaatat gaggaatgtc ttgaaaagaa
     4381 catatctttt gatgtatata tccaagattt tatggataat ttagagattg aacctaatga
     4441 tatgagttca gtgctattaa acataataga caatgcaata gaatctttgt ctgctataca
     4501 aaaggaaaag aagtatataa gattacatgc atatgaagat gaatttcagt atattatagc
     4561 cataaaaaat aatggtccta aaatacataa tgtcgaaagt atatttttgg atggatttag
     4621 cactaaagaa gggcaagaca gaggttttgg gttatatcta gttaaaaata tactagataa
     4681 gtatgattat agtatacttg taaatagtga cgatttttta actgaatttg taattttaat
     4741 accaaaatat tattagttag gaggagagaa gtctgtgata aaagttttga tttgtgatga
     4801 tgataaaatt gtaagagatg atatagcaaa tatagttaaa gagtccggat atgtagatta
     4861 tgttaagaaa gtcaaggatg gtatagaagc aattgaattt ataaaaagtg agaaaataga
     4921 cttattgatt attgacattg atatgccata taaaaatggg attgattctg ccaaagaaat
     4981 tctttctata gataaagata tacatatagt ttttgtaaca ggttttgctg actatagtct
     5041 tgagagcttt aaagtacatc ctatagattt tatagttaag ccgtttaaaa aagaaaaaat
     5101 acttgattca attaatatag caatagacca cattaactct cataaaattg caaaaaataa
     5161 ttttatagaa gatactcttt ttgtatataa aataaggaaa cagatacata tgattaattt
     5221 tgatgatata gtcatgtttg agaaaaattc aagagctatt aatttatata ctagagatga
     5281 agatacgata aaattttatg agaactttga tgatttaaaa aaacgcctac ctcctaattt
     5341 ctttatgact catagacaat acatagtaaa tttaagactt atacataagg ttatacctat
     5401 aaataagtct tctttagaga taaaatttat aaattttcaa gaaagtgcta ctttaaataa
     5461 atctttagaa aaagattttc tatacagatt ttatagggtt aaaaggtttt aatgaagaaa
     5521 aaaattttca gtgatataca atatgtatga tgaagatatc atataaattt gctatagtta
     5581 taaattaatt cgtataaaat ataatataaa acatattgga ctttcatgga aaattagaaa
     5641 gcccaatatg ttttagagat aaatcttata tttataagtt gcttaagata ctagggtctt
     5701 taatcttatt atcttcagca agtaataaga ttttagacat aatctcagca gtcttagggt
     5761 cttcatcagc gaatggaagg aatatcttac ctctactttg agaatgtaca ggaagtatat
     5821 tcaaagcacc catccctttt ttatgtacaa taccagaacc catatgcaca gaatactcac
     5881 ctaaattacc tgtgataaga acatgagagc cttcaaaatt gatattgtta atttttaata
     5941 ataataaaag ttctttagca attgctatac gcatctctac agtagaatgg ctagtttcag
     6001 ggtcaacacc accagcatga gcgacactta caacaaggtc tatatcacgc ataacttctg
     6061 agaatagaat tggaggtata gaagctaatt ctaaactttt ataagttttt ctatcaaaaa
     6121 attctactgt ttcaatagta ggaggctcta tatctgctgg tgaaaaccaa tctgcaagtg
     6181 caaacatctt tgccactata ttttctttat aaaatacttt ctgtaatccc tcttcataat
     6241 cgacagtcca aagctgattt ctaagaagtg ctacagtttt ctttggctga atttggtgac
     6301 cagcatatct gcgagatata gttttttcag atatttcatc agaatttatt ctataatact
     6361 cacggaatac ttgcttaaat ggttgaacaa gttgcttact aaataaatca tgttggaact
     6421 cactccactt accagattca aacaaatgaa ctggatgtgc aagttttaat ttattttttg
     6481 ctgtcaactt ataagtttca ccttctatat tgcaaataaa gaacttagac ttatcattat
     6541 aaacatatcc aatattatca tcacataccc atactagatt tttaatcaaa gggcagataa
     6601 ctggattttt atagatattg gcaatttctt taactgtaaa tactatttca tttttcatag
     6661 cttgttctaa actctgtttt gcacgagaat actgactctt taaatctttt tggacagatt
     6721 taagttcaag tacatatgga tgtttagcaa tagcttttgg tatactcttt aaagacttgt
     6781 tgcctttttg aactttagta gatgcttttc cgctttcatc aacatctatc caaattgaag
     6841 taccatcaat atcttttggt ttaaatagat gagcaacttc atttagtttt tcagtctcaa
     6901 gagcccacat catacgattt acatctccaa atccagcact tctagctaga ttttctagcg
     6961 ctatagtaca agcttttgat tcactttcac gacgttgtgc accaaattgc ttgctttctt
     7021 tagcaaactt ttgaataaat tcataacgga aagttgcttc ttttaggtct ccttttttca
     7081 taggaagtaa agagaaactt ctcaatttat cttgatttct atttgtttca atttctttca
     7141 tcaacatgtc tttatcaagc ttaccaagtg tagcatcagc atagagttgt gcacgtctat
     7201 gagagttacc accactagtt atatatttag ctgacttgta aagtatattg aatctttctt
     7261 ccccaatagt tttatatgaa tctaagaacc aatctacatc aaatgcacca tcaatgaaac
     7321 tacgtggtga aataggggag aatagagcaa cctctgattc tttttgtgca gagaagcctt
     7381 catttatatg tgcatggaag taccaaattg tacttttcag acccttccag tttaaatatt
     7441 tttcaattat gtttacccat tgaggggcat acatagcaga ttctattaat cttaactcag
     7501 taatgtcggt atttttaagt aaacttttta atttttttga atcatcttct ttagtaggat
     7561 agcaattttt aagtagagaa cttaaaacat ctttttttga ataattgcta ccatatgaat
     7621 atcctctctg gaaagtttct ttaccaagag cgaccaaaat atttacaaaa taatctatac
     7681 cttcaaagta ttgtatatta ttagctagat gagacacttc tgttggcata tcacctctac
     7741 gtgattcaat ttctataatt cggtttgtaa cagtttttaa attatcttta agccaagggt
     7801 acttttctaa tgtgtcacca gcccatctag gatttgttaa tgttcttatt atatttgatg
     7861 cgttgtatcc acttatcatt tctctattaa cttgctcttc agtcattaaa cctaattcat
     7921 gtgcacgagc aaacacattt atgctagtat agaaaggaaa cttatattct agcaactgat
     7981 aatttgaaaa cgtagtgtag aaaaacttag taaaagaaat atcatcataa ataaacgaat
     8041 gtgctttatc aagccaatag tttaaaatag gggcatttaa agcagggcag tattcatttt
     8101 gtctgtaata gtttttagta aattcaactc tttgtttttt acattgttct atagtcaata
     8161 agtttctaag tttatagtat atttctaaac aatagttaaa tatttcttta tggtcaaact
     8221 catttagtaa aactcttaaa agttttcttg taagtccatt aaatttaaat ttttcaagtt
     8281 ttgtatatat tttattttgt tttttagagt caaggacatt tgaatatata gatgaaaacc
     8341 aacttttttc tgtttcatta tatccacatg tacttatact ataaaataag atatcaagta
     8401 ttaatttgta atctaagtca agctttttac tagtttctct ccaaagttct gccaatggat
     8461 aattatcaag agtatcatta gaaactttat atttaatagg agtcaagtta taagtagaat
     8521 aacctaaaag cactttgtct acattacctg accatctttc aacttcatat tcataatctg
     8581 cattttcagt aataagctca tcaaattttt cataaacttt tattatttta tttatatccg
     8641 tatcgagaat tttttttgct tctttttttg ttgctacaga aacagaatcc tcactagtag
     8701 ataaacttat atttgaaata attttattaa gctttgatga gctattagaa tcatctttaa
     8761 aatcaaattg ttctggaatt tgaactttgt tttcagggtt atatattcca aaaccatttt
     8821 ctttgcagta atcatttttt gctttaccta acaaattatc aattaatatt tgaacttgag
     8881 atgtattctt tttagattta tcaatatcct ctataagtaa aataagctta ttatatagtt
     8941 cttgtctatc agaattgtct tttaataagt ttacaatttc aagacctcca aggcattttt
     9001 gagaatttga agattttatc agtgaagaag caatattttc caaatttgaa tcatcttgtt
     9061 caagtaaaat ttttattact gtttgtctaa tagagcttgt ctttaaagac aatagattgg
     9121 ttatcttatc taattctaca tctgttagct caagctttct aagtaaactc aataccgttt
     9181 ctctattgga aacactctta tcacttaaac attccaataa aaattcacgt tgtctatctg
     9241 tctttggttc atttacaaaa ttgactataa gtgaagctct taaatctgaa ctcataagat
     9301 gacaattttc cataagcaca tctataaatt gagcattcat atcgtatgct gttaaagtca
     9361 tcatacattt aagtatttca tgagaagaaa tttcaatcca agtccatgga aatacacttc
     9421 ctgaaaatga ttttgtcttt gaatttaaaa tttctaaagt atgttttaaa ctttcaaatt
     9481 gttcttttct agcatcaaag tctttgtcaa taggagtgtt tgacaagtca aaattagttt
     9541 ctctgttttg ccaactatag tagtagccaa cagaaccttc tacatagaaa tttgtaatta
     9601 cccatgctag tatctctaaa tcatcaccct taatgttttt agatgctata gaatgtttaa
     9661 aatatgtact ttctgtatgc gataaaaagt aaagagctac aagtttttta tatttttcct
     9721 gacaagaaag taagttttca ataagagagt aagagtcata tatgttgtaa gttgcagtag
     9781 cccataaggc aaaataaatt tcaagattat cattgctatt aattgattct atacggaact
     9841 tttcatcagt caatgattta tatgaaagtt ctaaacattt acgtattatt ccaggttttt
     9901 ggtctgaaaa accaagtcct gtccatgtat ccaaggctct tataacagaa ctaaatcgct
     9961 ctaaattgtt gtccaaaata gtttttaaca tgtatattga tgcagaaatt gtaccctcat
    10021 ctaaactctc tacaatttgc tgtctaagtc cttcttgttg tttggctgcc aaaagaagtt
    10081 tagaaagcat ttcatagcat ttatcactat gactcttcat gataccaatt atcatttgtc
    10141 ttgtaagtag agcagtttga ttttctccaa atattacttc ttctaaggca cttgttactt
    10201 tttgatttcc tttgtctatt tctagtgcta ttttatcact cattatagag tcatagtaca
    10261 agaccttatt tggaagagta agataatcta taactgaaaa atcataataa attaaatcaa
    10321 catagtttga aataaacctt ataatacctc taaaataatc cacaagttta tttgagcgat
    10381 accctttacg ataatatcct cctgaatatg tataatccat ctttctttta attaattggc
    10441 aaagttcttc tacttgttca gaagtaaata ttaattctaa tgcatttttt aaaggtccag
    10501 aaaggagctc gtccatattt ttaatttcgt agtagtggca attgtagtat ttctttatca
    10561 tatttaagaa tttgtcctca ggttctctat aatagtatga actattttct atgcagtccc
    10621 aaagagattt tgctaagtcg tagtactgtt ttgaagaaga tttagtaaaa ttatttttaa
    10681 aattttcaat tattttagga tttttttcta ctctagtacg ttctaacatt tatgtacctc
    10741 caaattattt attttaaaac attcttccag caagcaaggt aagtcttata aaagtaaata
    10801 tatctccact tttcagattt ataggggaat cgagtttttc aataacatct tcatttataa
    10861 gcactttgta gattccatct tcaaatgcct gtaacgctac ttctattgcc ttttctacag
    10921 attgtttatt ctcattgtaa atagagccaa atccaacttt tccaatatca gcttgagatt
    10981 caatttcttc attagttaag aagtttatta ttgatttttc tgggattttc tcattatatt
    11041 caagaacatt tttagataca atttcagaga tagcttcttt taggcaattg atgttgtcag
    11101 atacatcaaa aggggtaagt tctaacatag gtttgcgttt gccaacagcc ttaactctaa
    11161 aataaatttg cataaaatca cctcgcaaaa tattaattat gtatttagaa atttaaaatt
    11221 tatctatttg ttatattata tcattttgtg aattattaat gtgaattatg gaattatttt
    11281 acaaaaaata gaaaaaaagc tatcttaaag tctaaaaaag caataaaaga taacttttaa
    11341 ctataattat atagaattat aactgtacat ttattaaatt ttaattttag aattattttt
    11401 gataactatc tttttttatt tataaatttt gttcctaaat attttttgat atcttcttca
    11461 ggagaaaagt catcaataag tatttctttc ccagtgtctt caaatactac aagaacttca
    11521 cagtcacctt gcaagaaaaa ttttctaact gtatcttctt taacttctct aacttttatt
    11581 acattctttt tttctttttc aaagtctttt gtgtaataac ttcccatcat attaattatt
    11641 tttgtatatc tattcataaa atacctccat atgcgattaa atttaaaaaa tatgttataa
    11701 taattgtttg tattaactta ataattataa caaggagtaa taataatgac tagtataaaa
    11761 aataaagata ttttaaattg tatagattat tccattaaaa ataagctctt tgacaaacta
    11821 aatgatgtat ataagtcctt gccagtagga aattgttctg gatgtggaaa ttgttgtatg
    11881 gagtcagtag gtataaattt aattgagttt ttgaatatat tttgttatct tgaagataga
    11941 gttgatttaa aaaaaagctg tataagtaaa atattagatt attattttga ggagtataca
    12001 agaaagaacc catgtccatt taaagatact aataatagat gtctaatata tgaagttaga
    12061 ccattaaatt gtaggatgtt tggacattgg aaaaaagagg attataataa aaatttagat
    12121 aatgttattg agaaaaataa taactataga gagttaatga aaaagcaata tggatttgaa
    12181 ataaatgatg aagtagtaaa ttacaggata gattattgtg atagatttat tccaaataaa
    12241 gattatttaa gtaaatctaa gagattaagc ttttttgatg agcttatgat attagactca
    12301 aatatttatt caagtggaaa tatagatata gactttaggg atagaggtat agttgaatat
    12361 tttatagagt cattacttta tagaaattta gcatataatg tgaaaataag gatatctaaa
    12421 gaatacaaaa taagaaaaag aactatagat agaataaaaa gaattgttct tgtatagttt
    12481 tatattagta agtaataaat tgtatcataa atattgtcaa aatgaggtga ggaaggtgaa
    12541 tcttacaaaa gtaaaaggaa ataccttttt tataaaaggt ggcacgaata caggaatata
    12601 tgtatttgaa gataacacag cacttatgat agacccaggg ttatgtggtt caagacctag
    12661 gagagtaaat aaagtattag atgaaaataa tatacaggtt agatatatta taaatactca
    12721 tggtcatgat gaccactatg gagcttgtaa tcaatttaaa gaatattata aagatgtgtg
    12781 tataatgtca tctgaatacg acaaattata tatagaaaat ccagaattgt tttcaaagta
    12841 tattgttggg ggaaagagca atgtatttat ggataatatc ttaaaaaata aaatgttgga
    12901 tgatataaag atagataaat ctatagaagt tggaaaaatt gaattaaaca atgaaatgtt
    12961 tgatataatt gagtttcagg gtcatacacc tggtagtata gctgttttga cgaaggataa
    13021 tgtgattttt gttggcgatt tattgatagg agatgaaatt ctatcaaaat acgatttctt
    13081 gtttctatat gatatacaag aacaaataaa ttcattagaa aaattaaaaa atatagattt
    13141 tgagtactta gttttagggc atggcaagca agttatatcg aaagaagatt cttataaatt
    13201 aatagaaaaa catttgaatg ctttaaaaaa atatttatat caaataagag agttgcttgt
    13261 agaaccgaaa tctttagaaa ttttattaaa aaacattata aataacaata atttaagcaa
    13321 taattataaa gagtatcatt tttttaaatc ttcgttagta tctgtcataa gttacttagt
    13381 tgatttagaa gaaatagggt atatattgga aaatggagaa ttgctttact atacaaaaac
    13441 aaaataaatt atgataaaat aaagaaggtc ataaaaaaca aataaagggt gagcaatatg
    13501 gaatacgaaa aatggaatga gatactagca ccttatgaac atgcagtaga ggaattgaaa
    13561 ataaaattta aaaatataag aaaagaatat ttagcaaaag gtgaatattc tcccatagag
    13621 tttgtcacag gcagaacaaa aaaaatatct tccataatat ctaagttaaa aaggataaat
    13681 gcaaaagata tagaaactga aattgatgat attgctggta taagaataat gtgccagttt
    13741 gttgaggata tatatgctat agttgatttg ataaaagtaa gaaatgatat gactataata
    13801 ggagaaaaag attatattac aaattacaaa gatagtggtt atagaagcta tcatgttata
    13861 ataaaatacc ctataaactc cattgcgggt tcaaaggaaa ttatatgtga aatacaaata
    13921 agaactcttg caatgaattt ctgggctaca atagaacatt cattaaaata taaatatgac
    13981 cattacatac ctgagactct tgctgaaagg cttagaagag catctgatgc tgcctttctt
    14041 ttagaccaag aaatgagtga aataagagag gacataatga aagctcaggc tatgtaccaa
    14101 atgaagtcta tagctattag agatactcta aatagaatac aagagttata tgacttgggt
    14161 gatacacata aagctatgca gtaccaaaga aaacttgata gaactgaaac agataaaaat
    14221 ataactgaaa ttttggaatt aaaacaagaa atagatatat tactagagca atataaggat
    14281 actaaggtag atgataaagt agttcagtaa aaaactactt gtagaaaatt tgctaaatat
    14341 taatggggct gcctcttaat ggaaaataat ttctaaaaag agacagccta tttagtgtaa
    14401 ttaagaaagg aatgatatta tgagtttgta tgctataggt gatttacatt tttcaacatc
    14461 agtcaataag ccaatgaata tctttggaag taattgggat ggtcatgaaa agaaaattat
    14521 agataattgg aaagaagttg ttaaggaaga agatatggtc ttagtactag gagatacatc
    14581 ttggggaatt aatttatcgg aagctaaaaa agatttagat ataataagca aacttccagg
    14641 tcagaaaata cttataaaag gaaatcatga ttactggtgg acaaccgtta caagtttaaa
    14701 taagctatat gaagacatgc gttttattca gacaaatttt tatgaatata aagattatgc
    14761 aatttgtgga ggtaggggct ggatatgtcc taatgatgtt aaatttgatg agactgatga
    14821 aaaagtttat aaaagagaag agcatagact tagactatca ttagaatctg ctcgtaaaag
    14881 tggtcactca aaaataattg tgattactca ttatcctcct acgaatgata aattggaaga
    14941 atctttattt acaaagttat ttgaagaata taatgttgaa aaggtgattt atggtcactt
    15001 gcatggtaaa gaatctttta aaatgggatt gaaggggatt cgaaatggag tagaatatac
    15061 attagcatct tgtgactata cagaatttaa tttaataaaa gttcacgatt aaaaaacata
    15121 aattggtaaa tactataaga tacatgatta aaaataaata tttgagggat ttcggttttg
    15181 agaaaggaga cacgacttat gaggtggata ttagttttta tatttttata tcttattcct
    15241 ttagtagtga tattcagaaa ttacaagaat tttaagaggt cttgtatcta cagtagtatt
    15301 tatgtggtat tagctacaac aataatgata agtaatattt atatgagtgg acttaataaa
    15361 ataaaagagt ctatgtatta tcacaattat atttctgatg agacatatga tgaaaagtat
    15421 gtatcaaatt ttaaggaaaa agaaagtacc gatttaaaac aggataataa aaagaatgaa
    15481 gaaatatcaa ataat
//
LOCUS       DAEPOZ010000083        15344 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.083, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000083 DAEPOZ010000000
VERSION     DAEPOZ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15344
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.083"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            130..1611
                     /locus_tag="KR440_001879"
     CDS             130..1611
                     /locus_tag="KR440_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0698930.1"
                     /translation="MKKNRLIIKNISIAFIVLFFFSVFTFFYIGNISRVLEYETNDIM
                     TVTITGWIILSFLFLCIIIYILYSKAKSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTNINETFARVSAD
                     NFNILLTYNKKEDIINIIKKIIANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLLEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFLKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            1644..5081
                     /locus_tag="KR440_001880"
     CDS             1644..5081
                     /locus_tag="KR440_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBH0698931.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPIFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLIIYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEISKQL
                     YKNKTIELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENILGGIIITEFNDDFTIKYTNSGYLSMIGYTREQLENELKYKACNLVFY
                     EDRKKALESIQHQLNTKGIAVYQHRIRRRDGNIIWVSIKGRLIIDDDGIEKGIWILND
                     ITDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNV
                     PYSLVESGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDE
                     DGNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESW
                     QKPFGIKPDNNYSEMVKTISNSIIHKDNKEVFLNTFLCKNLLSAYNKNITEIKLEYRR
                     MNQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIKLKLKAERDTLTGLYN
                     KGTTENLINDFLNSDEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKHLKPI
                     FRKDDIVGRIGGDEFIVFLKNIQSFENTKKKAEKICDIFRNSYTENKKTYKISGTVGI
                     SFVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNNCIYQNELENNISDNNIKLANK
                     CEKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSER
                     PCNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            5099..5845
                     /locus_tag="KR440_001881"
     CDS             5099..5845
                     /locus_tag="KR440_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBH0698932.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNSIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQRKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(5794..6114)
                     /locus_tag="KR440_001882"
     CDS             complement(5794..6114)
                     /locus_tag="KR440_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBH0698933.1"
                     /translation="MEKIMNFLKENSTFYFATVEDNKPRVRSFGFIMDYEGKLYFGIV
                     KYKTSYKQLLVNPNVEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAK
                     NSLV"
     gene            6905..11416
                     /locus_tag="KR440_001883"
     CDS             6905..11416
                     /locus_tag="KR440_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HBH0698934.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISSVTNPP
                     IGSTNLDGTTPNTLLITVAGSDIINQNILNGPVIYTPIENILDSCVSVSNINLITDAD
                     NGTFGFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSN
                     VIGAWWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVT
                     GYPPPQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFS
                     EGESAIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTN
                     VFFRDLIPNGLEFIPGTVTVDGVSVPGVDPNTGFPLPDIGGGIGVEVTFDVVVESIPN
                     PNPTNNIATIDYSYTPVEGGIPNDFSVDSNPVPVEVISADVEVTKLSEPTIVNPGEEL
                     IYTIKVVNNGPFPSENVVLTDDVPASIINPEYSLDGGVTFQPWTGSLNIGTLEVGETR
                     VIIIRGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNS
                     AVPGTLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGT
                     LNSGISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISII
                     KVADEDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWN
                     GSLNIGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSA
                     DIEVIKTSNMDTAVPGETFSYTITVINLGPSAAQSIVLTDDIPDVILNPEYSLDGGVT
                     FQPWNGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSPTPDPNPDNNTSTDE
                     TDISPLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFAENVTLTDNIPASILNPEYS
                     IDNGVTFQPWNGSLNIGTLDAGEIRNIIIRGTVSQTAIGTIINTATVSSTTPDPNLNN
                     NTSTSEAEVSSSADISVVKRSNQTVVVPGDVLDYTIEVRNAGPSTAQNVTLTDNIPAS
                     ILSPEYSIDNGVTFQPWNGSLSIGTLDAGEIRNIIIRGIVSQTAIGTIINTATVSSTT
                     PDPNLNNNTSTSEAEVSSSADISVVKRSNQTVVVPGDVLDYTIEVINAGPSTAQNVTL
                     TDNIPASILSPEYSIDNGVTFQPWNGSLSIGTLGAGEIRNIIIRGIVSQTAIGTIINT
                     ATVSSTTPDPNLNNNTSTSEIDISSSADISVIKLANKTEACVEEQIDFVIVVSNAGPE
                     NAQNVTLIDNVSDKLKKAVFSLDRGVSFQPWTGSLNIGTLPAGTLRVILLRGIIKSTC
                     LDRLTNIAEVTSTTPDSNLNNNISRVQVEIKQCCCNDCCCNNCSNCCCNDCGCKDCCC
                     NCCKNDFWC"
     gene            11873..12220
                     /locus_tag="KR440_001884"
     CDS             11873..12220
                     /locus_tag="KR440_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBH0698935.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
     gene            complement(12370..13419)
                     /locus_tag="KR440_001885"
     CDS             complement(12370..13419)
                     /locus_tag="KR440_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0698936.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKDESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQFGVMAGAFAGGEGDFTTAFEPTATAMEKEGS
                     GYIVTSIGKDSGEIPFTAYSTTRSYMKNNKDLIQRFTNAVYKGQLWVQQASSKEVAKA
                     MQPFFEDISLEDLTTVVDRYKSIDAWSQNPVLKEEGLKKLMTVMKEAKELDKEAPYKD
                     IVNTEFANNAIKNIK"
     gene            13805..14539
                     /locus_tag="KR440_001886"
     CDS             13805..14539
                     /locus_tag="KR440_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH0698937.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGNNLHWPVDRYFEEGVRKSNFLGEEVIKYHKTLTTYLNTLMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(14681..15172)
                     /locus_tag="KR440_001887"
     CDS             complement(14681..15172)
                     /locus_tag="KR440_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBH0698938.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
BASE COUNT         6041 a         1895 c         2320 g         5088 t
ORIGIN      
        1 gataatgata tttatttaaa tgctctttat taacaattta taaaacttaa ttgtaaaaaa
       61 tacttattaa tagtaaaata aagtgaatca attatattat aattgctaaa gagagtgagg
      121 tgtagctatt tgaaaaaaaa tagattaata ataaagaata tatcaatagc atttatagta
      181 ctattctttt ttagtgtatt tacattcttt tatataggta atattagtag agtattagaa
      241 tatgaaacaa atgacataat gacagttaca attactggat ggattatact ttcattttta
      301 tttttatgta ttataattta catattatat tcaaaagcaa agagtcaaaa aactatagaa
      361 aaagtagcat atacggattt tgtaacaggt tattccaact ggagaaagtt tgaattagat
      421 gttacaaatt tattaaagaa aacatcacaa aataataaat atgcaatggt catattcgat
      481 attgataaat ttaaagcaat taatgatata tatggacata aaaagggcaa tttaatatta
      541 aaagatattg cagatacttt aaatgaattg actaatatca atgaaacttt tgctcgtgta
      601 agtgctgata attttaatat attattaaca tataacaaaa aagaggatat aataaatatt
      661 attaaaaaaa ttatagcaaa taatgaactt gtaaacttat cttttggaat ttatgaaatc
      721 aaagataagg atttatctgt aagtgtatat agtgatagag cttcacttgc aaaatcatca
      781 ataaaaaata atagtgatgt aaattttgca ttttttaatg ataaattaag agagaaatta
      841 ttacttgaag ataagataga aaaagaaatg gaatatgctc ttgaaagtgg gcaattcgta
      901 atgtatttac aaccaaaata taatattaaa ttagataaat tttgtggaag tgaagcccta
      961 gtcagatggc aatatacaga aaaagaagtc atatatccag gtgattttat tcctatattt
     1021 gaaaaaaatg gatttattag aaaaattgat atgtacatat tagaacaagc ttgtaaagaa
     1081 atccggtctt tatttgataa aggaatttca cctttaccta tatctgtaaa cttctcaaga
     1141 gttgattttt taaaaaaaga ttttattgaa aacatagtaa atatatgtga tagatataaa
     1201 ataccatata gtttaataga aattgaaatt actgaatcta gtatgtttgg agatactgat
     1261 actctattta atgttagtag aaacctacag gatattggtt ttatagttgc aatggatgat
     1321 tttggctcgg gatattcttc tgtaaatatg cttaaaaata taccattaaa tgtaataaaa
     1381 ttagatagag gattttttgt tgatgataaa gatgttgata agagtcaaat tgttataaaa
     1441 agcatagttt ctctcattaa gcaattagga ataagggttg ttgctgaagg aattgaaaca
     1501 agaagtcaaa tagaaatgtt aaaaaaagca aattgtgata ttgttcaagg atattatttt
     1561 tcaaaaccat taccaattaa agaatttgaa aaattagtct ataaaatata gtcagcgagt
     1621 cttaaatggg aggtggtgat taagtgataa gagcaaaatc gttaaaaaat gtactgagta
     1681 ttgttttaat atttttattt acaataattt ctttttctga ttttgcattt aaaactaatg
     1741 tccaggaaga agaaaatagt aaaattaatt taagggaaat atccctacaa agttccaatg
     1801 ctataaaaat aaaattagat agttctctat cctttattaa aagtgtggca aatatggttt
     1861 cttattttaa tactatagat aactcaaaaa tagttccatt attagctaac atatcaaaag
     1921 aatctgactt tcaaagaatc tctattgtat ctcctgatgg aataagtcat accagcgatg
     1981 gcaaaattat caatgtgtca aaatatcagt attttaagaa agctattaaa ggagaaagca
     2041 atatatctga agttgtgaaa tctccaattg atggaaaaga tacttttgtt atagctactc
     2101 ctatatttaa taagaataac aaaataattg gtgttttata cggttcttac tacaatgata
     2161 aattagctga atttatagat ataacaagtt ttaatagtga agggtacata gatatatttg
     2221 aatctagtgg tcaatttgtt ttaaaatctt cacatacaaa caaactgatt aagtcaagta
     2281 gtaacatata tgatatattt aaaaaagctt cttttgatga tggttatagc tataaaaaaa
     2341 tatatgaaga tacaaaaaat aacaagagtg gctttacctc atatgaatac aaaggagaaa
     2401 atcgttatgc tacttataca cccattggtg taaatgactg gtatgtaatt tctgtgatac
     2461 cacaatatac tgtaagaaga aacattgata aaataaatca attagcattt tctcttattt
     2521 tcaaagtatt cttattgttt attatgctta taatatatat tatttataaa aacaataaat
     2581 ctcagaaaga tatactaaaa gctaacaaag aaatctcatc tattacaaat agcattcctg
     2641 gaggagtaca aaagtgtacc ctgggtgaaa catgcaatat taatttttta agtgatggat
     2701 ttatagagtt gtttggttat acaagagaag aaataaaaac attgtttaat aatgattttt
     2761 atagtttaat acataaagat gatgttgaaa gaataaaaaa agaaatttct aaacaacttt
     2821 acaaaaataa aactattgaa ttagaatata ggattataac aaaaaatggt aatacagtat
     2881 gggttttaga taaaggagaa cttataacag aacctgatgg aaaagaatat ttttgttgtg
     2941 taactgtaga tattactaga acaaaacaat tagaacaaga gatggaacaa aaaaatctag
     3001 aacttgaaat gatatcagaa aatattttag gtggtataat tataacagaa tttaatgatg
     3061 attttactat aaaatacact aactctggtt atctttcaat gattggatat acacgtgaac
     3121 aattagaaaa tgaacttaaa tacaaagctt gtaatcttgt tttttatgaa gatagaaaga
     3181 aggctcttga aagtatacaa catcaattaa acacaaaagg aatagctgta tatcaacata
     3241 gaataagaag aagagatggt aatattatct gggtttctat aaaaggaagg cttattattg
     3301 atgatgatgg tattgaaaaa ggtatttgga ttctaaatga cataacagat ttaaaaaatg
     3361 cagaacaaaa acttaaaata aatgaagaaa gatttagaat tgcattaaag caaacatcaa
     3421 atactatatt tgagtatgat ataaaaacaa agtcactttt aaatactagt aaagagcttg
     3481 ataaatataa tatttataaa attactaaaa acgttccata ttctctagta gaatcaggag
     3541 ttattcataa agattttagt gatgatttta taaacatgta tgaaaatgta ataaatggag
     3601 caaaaaaagc aagttgtgta gttaagatga aacttccaga tgaaaattat atatggaata
     3661 aagttacctt gactactatt tttgatgaag atggtaatcc tgtaagagca attggtatac
     3721 aggaagatat aagcaaacaa aaggaagcag agataagata taatcaagaa ggtaagtatc
     3781 attcagctat attgtcggaa gcaatgataa cctatgaaat aaatgtatca aaagatactt
     3841 ttatcaaagg aaatgaaagt tggcaaaaac catttggaat taagcctgac aataattatt
     3901 ctgaaatggt aaaaactata tcaaacagta ttattcacaa agacaataaa gaggtgtttt
     3961 taaatacatt tttgtgtaaa aatttattat ctgcatataa caaaaatata actgaaataa
     4021 aattagaata tcgaagaatg aatcaagaaa ataaaatgat ttgggtcatt tgcacaatgc
     4081 acttaataaa agattctatt acgaatgata taaaagcact tgcttatgtt aaagatattg
     4141 acaagcaaaa aaatgaagaa attaaattaa aattaaaagc tgaaagagat actcttacag
     4201 gattatataa taaaggtact acagaaaatt tgattaatga tttcttgaac tctgacgaag
     4261 caagcttagg aatgcatgca tttttaattg ttgatataga taattttaaa tctgtaaatg
     4321 ataatttagg acatgtattt ggagataaag tattatttga agtttctaaa cacctaaaac
     4381 ctatatttag aaaagatgat atagtaggtc gaattggtgg agatgaattt atagtattct
     4441 taaaaaatat tcagtctttt gagaatacca aaaagaaagc agaaaaaata tgcgatatat
     4501 ttagaaattc ttatacggaa aataaaaaga catataaaat atctggaaca gttggaattt
     4561 catttgtacc agaacatgga actgtatttg aagaacttta taaaaaagca gacatagcac
     4621 tttattatgc aaagagcaaa ggaaaagata agtatgcact ttataataat tgtatctacc
     4681 aaaatgaact tgaaaataat ataagtgata ataatataaa attagcaaat aaatgtgaga
     4741 aatccaaacc agtacttaaa gaaattcttg attctattga taattatatt tatatagtga
     4801 atccaaatac ttatgaactt ctttttgtca ataataaggt gtctcgtttg gatttagaga
     4861 taaaattagg agaaacctgt tataaatcac tattaaaatc tgagagacca tgtaataatt
     4921 gccctcttaa cggattagaa gaaaataagc attcaaaatt caaaatagag acaaatacat
     4981 ttggtaaaaa tatcagtata gcaagttgga ttaattgcat agatggagaa tacaactgtc
     5041 ttatagagtg tatagattct tctttataca aaaataaata aaaaaggact atgaagatat
     5101 gttaaatagt ataactttga tacttggtat aatatttaca atttactatt tttatctcaa
     5161 aatagttttt ggaggaattt catttagtga gattttctta gttgtaggaa ttgtactaat
     5221 tatctatcaa atatttaaga aaaaaattaa agaaaagaag gttttttata gggtgttaaa
     5281 aataataatc agcatttttt taatagtatt tgtagttact gagagtttaa ttatttttta
     5341 tcctaaaaat agtttagaat caaaaagtga ttatctctta attttaggag cttctgtaaa
     5401 aaagactaca ccaagtacaa ccctgaaggg taggttagac actgcattga aatacctaaa
     5461 aattaatgat aattgttatg tggtagtatc tggtggtaaa ggaagtggag aaaaaataac
     5521 tgaggcaaaa gctatgaaag attatcttat aaaaaatagt atagataaaa ataggataat
     5581 agaagaggat aaatccacga atacttatga gaattttaaa tattcaaaat tgaagataga
     5641 agaacatagt caaagaaaac taagtgattt aaaagtaaaa atagtaacaa cagattttca
     5701 tgttctcaga agtaaaatat tagcatatag aaatggatat aaaaatacgt ctttttatgc
     5761 tagtaaatca aaactttcat ttgtaccaac ttactataca agagaatttt ttgcattatg
     5821 gaaaacaatc atttttgata gataataaat aaaaagacta gatataaact catatctagt
     5881 cttttctatt cccttaattc taatccattt tccaacttta cttgttgtag aaatttcaac
     5941 attgggattt acaagtaatt gcttataaga agttttatat tttacgattc caaaatataa
     6001 ttttccttca taatccatta taaatccgaa tgatcttact cttggtttat tatcttcaac
     6061 tgtagcaaaa tagaatgttg agttttcttt taaaaaattc attatttttt ccatgattaa
     6121 atcctctttg tatcttatac taatataatg aatataaata aaatatttat aatactaata
     6181 taaattgata taaaaacaat tacaatatta tattgattat tcaaaattat atataaaatt
     6241 cagaaaaaca tttcagttta cataataaaa ataaagtaaa ctaaaattat tgattttaga
     6301 ttaaagtgcc agattaagta tttaagtaga ttttaagctt gatttgatta gaaaggtaca
     6361 aactatcacc gaaaaaaatt aaacccattt aaaaagcaat ataaaagctt gttattttcg
     6421 agaaaaatag accctctaaa ctcaattaga taaaaataaa atacaaaaaa ttatatctct
     6481 gaatatctca agtaattaag tttaaatttt acgatatttt gtttgatgat ttataatata
     6541 aaagcatatt cttaatcttc tcaaattaaa aagagtaaaa aataacaaaa aaatcactga
     6601 catttcactg acaaattgta attttgttat tataaataca ttgaaaatac ttgattattg
     6661 aaaaatggca taaaataata ccttttatgc catttactat tgtttgatag tttttaactt
     6721 atgacataag atatttttta acatatacaa taaaatataa aaaaattgat attgcaaata
     6781 aaaaaaatac atattagatg ttatagaaaa aaagcgcact tataggctta aaatgtcaca
     6841 taaaagagtt tcaaagaata atatattatg aacacaaatt ttgtaatcac aaaggagtga
     6901 taatatggca acagtatcag gacaaatagt ttttgataga aatagaagtg ctaccattga
     6961 ttctggggac tcaggaatag ccaatatacc agttgtatta caagatatag caactggagt
     7021 tagacttgtg gttctcacag atgcatcagg aaattatgtt tttactaatg ttccaaatgg
     7081 taactataga attgtagaag cttttggaac tacaggagga gtaccaacac cagggaattt
     7141 tgcaacagcg atagtaggtc cagttccaat tgcagaaatg ccacctatta gttctgtgac
     7201 aaatcctcct attggttcaa caaacttaga tggtacaaca cctaacacac tattaataac
     7261 agtagcaggt tcagatataa taaatcaaaa tatattaaat gggcctgtga tttatacacc
     7321 tatagagaat atattggatt cttgcgtgtc tgtatctaat ataaacctta ttacagatgc
     7381 agataacgga acattcggtt tttttccacc aggtacacca agtaatacag gacctgaaat
     7441 agaaccttac ccaaatgtga cgccagattt tacgtatgtt gtaccagacc caactaagtt
     7501 tacaccttta gatggagaat atacagtaca aaatattatg acaaatgcaa tgagtaatgt
     7561 tataggagct tggtggcgta ttgctgacca tactacagga aatgaaacag gaagaatgat
     7621 ggtggtaaat ggttttaatc ctggagctgt attttttaaa tcaacagtac aagttacacc
     7681 taacactaat tatttattta gcacttggat tttaaacctt tttaaggtaa caggatatcc
     7741 accaccacag ctaggtgtaa gaatatttga ccaaaataat aatatactat atcaagcaac
     7801 attaggagct caaattccag ttaatacaaa cgcaccagaa tggaaagaag taggtactgt
     7861 gattaattct caaaataata atcaacttac agtagaattt tttagtgaag gagaaagcgc
     7921 cattggaaat gactatgcta tagatgatgt ttctttaaga gaaatagctg ttccaacctt
     7981 tactccaata aaaacaagta acacagaaac tgctaatgta ggtgatataa tcacatatac
     8041 agttacatta gataatactt gtgatagccc tttaactaat gtatttttta gggatttaat
     8101 tccaaatggt ttagagttta tacctggaac tgtaacggtt gatggagtat ctgtaccagg
     8161 agtagaccct aatacaggtt ttccacttcc agatattgga ggaggaatag gggttgaagt
     8221 aacttttgat gttgtggttg aaagtattcc taacccaaat cctactaata atatagctac
     8281 tatagattac tcctatacac ctgtagaagg cggtatacca aatgattttt cagtagattc
     8341 taatccagtt cctgttgaag taatctcagc agatgttgaa gttacaaaat tatctgaacc
     8401 tactatagtg aatccaggag aggaattaat atacactata aaagtagtaa ataatggacc
     8461 attcccatct gagaatgtgg ttttaacaga tgatgtacca gcttctatta taaatccaga
     8521 gtattcatta gatggaggag taacttttca accatggact ggaagtttaa atatagggac
     8581 attagaagta ggagagacaa gagttattat aattaggggt attgtaaatc catcaacagt
     8641 aggaattata acaaatacag ctgtagtatc ttcaactaca gcagacccaa accttaataa
     8701 taatacatct acaatagaga cagaagtgaa tttattagca gatattttag taatgaaaac
     8761 agcagaacct aattcagctg ttccaggtac tttattgaga tatactattc aagtagaaaa
     8821 cttaggacca gcaaatgctg aaaatgtaat attaaatgat gatattccag cctctattat
     8881 aaatccagaa tattcattgg atggaggagc aagttttcaa ccatggaatg gaagtcttaa
     8941 tataggaact ttaaactctg gaatttcatt aacagtttta attcaaggaa cagttagttt
     9001 aaattcttca gaatatatag taaatacagc aacagtttct tcaactacac ctgacccaga
     9061 tttaagcaac aatatatcta ccattattac accagttaac ccacaagcag gtatttctat
     9121 tataaaagta gctgacgagg atgtagctgt tccaggagaa gaatttgtat atacaattga
     9181 aatttttaat gaaggaccat ctaatgctac gaatgtagtt ttaacagatg atataccaga
     9241 tgttatttta aatccagaat attcattgga tggaggagca actttccaac catggaatgg
     9301 aagtcttaat ataggtacag ttgcaccagg gcaattaata agaattatca taaagggatt
     9361 agtaagttca actgctacag gagatattac taatacagca gaagtaagtg cagatgtacc
     9421 agaaccagtt acggattcat caacagtgac aactcctata gttccatcag cagatataga
     9481 agtaataaaa actagcaata tggatacagc agttccagga gaaactttta gttatactat
     9541 aacagttata aatttaggac catctgctgc acaaagtatc gttttgacag atgatatacc
     9601 agatgttatt ttaaatccag agtattcatt ggatggagga gtaactttcc aaccatggaa
     9661 tggaaatctt agtataggca ctttggatgc tggagaaata agaagtatta taataagagg
     9721 aacagtgagc cagacagcag taggtacaat tataaataca gcaacaataa gttcacctac
     9781 acctgaccca aatccagata ataatacttc aacagatgaa acagatataa gtccattagc
     9841 agatatttct gtgataaaag gcaacgaacc agttgctata ccagggggaa gatttattta
     9901 tggaattgaa attgcaaatg caggaccatc ttttgcagag aacgtaactt taacagataa
     9961 tataccagct tctattttaa atccagagta ctcaatagat aatggagtga ctttccaacc
    10021 atggaatgga agccttaata taggtacttt agatgctgga gagataagaa atatcataat
    10081 aaggggaaca gtaagtcaaa cagcaatagg tacaattata aatacagcaa cagtaagttc
    10141 aactacacct gacccaaatc taaataacaa tacttcaaca tcagaagcag aggtaagttc
    10201 atcagcagat atttctgtag taaaacgaag caatcagact gttgttgtac caggagatgt
    10261 actagactat acaattgagg tgagaaatgc aggaccgtct actgctcaga acgtaacttt
    10321 aacagataat ataccagctt ctattttaag tccagaatat tcaatagata atggagtgac
    10381 ttttcaacca tggaatggaa gtcttagtat aggcacttta gatgctggag agataagaaa
    10441 tattataata aggggaatag taagtcaaac agcaataggt acaattataa atacagcaac
    10501 agtaagttca actacacctg acccaaatct aaataacaat acttcaacat cagaagcaga
    10561 ggtaagttca tcagcagata tttctgtagt aaaacgaagc aatcagactg ttgtcgtacc
    10621 aggcgatgta ctagactata caattgaggt gataaatgca ggaccgtcta ctgctcagaa
    10681 cgtaacttta acagataata taccagcttc tattttaagt ccagaatatt caatagataa
    10741 tggagtgact tttcaaccat ggaatggaag tcttagtata ggcactttag gtgctggaga
    10801 gataagaaat atcataataa ggggaatagt aagtcaaaca gcaataggta caattataaa
    10861 tacagcaaca gtaagttcaa ctacacctga cccaaatcta aataacaata cttcaacatc
    10921 agaaatagat ataagttcat cagcagatat ttctgtaata aaattagcaa ataaaacaga
    10981 agcatgtgta gaagagcaaa tagattttgt aatagtagta tcaaatgcag gaccagaaaa
    11041 tgctcaaaat gttactttaa tagacaatgt ttcagataag cttaagaaag cagtattttc
    11101 acttgataga ggtgtaagtt tccaaccatg gactggaagt cttaatatag gtacactacc
    11161 agctggaact ttaagagtta ttctattaag aggaattata aaatcaacat gtcttgatag
    11221 attaacaaat attgcagaag ttacttcaac aacaccagat tcaaacctta acaataatat
    11281 atcaagagtg caagtagaaa taaaacaatg ttgttgtaat gattgctgtt gtaacaactg
    11341 tagtaattgt tgttgcaatg attgtggttg taaagattgt tgctgtaatt gttgtaaaaa
    11401 tgacttttgg tgttgatttt ataaaataat tttgtatacc ttaaattaaa ctgaaataaa
    11461 aaataaattc acatatgagt aaaatatttt ataaatatta ataaattttc tatctttgat
    11521 atggaccaca ttcgtaatac tagttattaa atgaagtggt cctttttaat tcattaaaat
    11581 tatttattct aattaaattt taaatgtatt aaataatttt aattttttat aaaaaatatt
    11641 catatattct ttatattaaa gtttagatat ctaaaaatac ttttagaatt tattatatta
    11701 tgttaatttt tttatatctt ataatgatta tatttttcta actataaaaa tagtatttac
    11761 ttatatttaa aaataaaaga attttattcc tattgtgtaa agtgagaata ctattttctt
    11821 atattggtat aataatagta aatctataat atagaaaaga ggtatttcta gtatgcatga
    11881 aaataaaaag aatctattag acggaagctt agctcaatat aatgatacag cagttccaaa
    11941 aataccagta tttgctggta acgatataac ttcagaagtt tattatttta aacctaatca
    12001 agttcttaat gctcatagac accctaatgg tgaacaaata tttgtgttcc taaaaggaga
    12061 aggtaaaatg aaacttggtg agcatgaatg tgatgtaaaa aacggcgata ctgtatttgt
    12121 accaacagga gagtggcatg aaattactaa tggaagcaat gaagaaatgg tagccgttca
    12181 aattacaaaa attaatgctg gagcagaata cagaggatag atatataagt acagttagta
    12241 ctttaatatc tattttatta tataaacttt aatctcaaat ataaaaagag tcttttaaaa
    12301 ggctcttttt atatttttgt taattttcat tataaaaaca agaaaatatc tagctaaaac
    12361 taatatattt tatttaatat tttttatagc attgtttgca aactcagtat ttacgatatc
    12421 tttataaggt gcttccttat ctagttcttt agcttctttc ataacagtca tcaatttctt
    12481 caaaccttcc tcctttaata caggattttg agaccatgca tctattgatt tatatctatc
    12541 tacaactgtt gttaaatctt ctaaagatat atcttcaaag aaaggttgca tagccttagc
    12601 tacttctttg ctagatgctt gttgaaccca taattgacct ttataaacag catttgtaaa
    12661 tctttgaatt aaatctttat tgtttttcat gtatgatctc gtagtagagt atgctgtaaa
    12721 aggtatttct ccagaatctt ttcctataga tgtaactata tatccactac cttctttttc
    12781 cattgctgtt gctgtaggtt caaatgctgt tgtaaagtca ccctctccac ctgcaaaagc
    12841 tccagccatt acaccaaatt gtacatcagt tcttacatta acattgcctg cttttagatt
    12901 ttctccaata gtaagacctt tttcttttag aacatattca aaagtcatta gtggaactcc
    12961 accttttcta ccacctaata tttctgttcc ttttaaatca ttgtaagaga aattaggcat
    13021 ttttttccta gcaactagaa aactaccatc tctttttgtc aattgagcaa agttcactgc
    13081 ataatcgttt ttgttttgat tatatacata tatagatgct tcaggaccca ttaaacctac
    13141 gtttgcttca cctgaaatca aagctgccat agttttatct gcaccttggg tatttataag
    13201 gtcaatcttt ataccttcat cttcaaagaa tcctttcgta atagcagcat actgtggagc
    13261 ataaaaaaca gagtgagtaa cttctgctat agttacttta tccagatttt tagacgaaga
    13321 agattcatct tttttgtcat tagagttaaa atcacatcca gttagacata ttgtacttac
    13381 aatgatagag cttgctattg cgactatttt tttaaacata ataaatacct cccataagac
    13441 atacgaatag tatatagtat taaaaattta ttgtagttgt tacaaaaatg tatctatgct
    13501 tataatattt ctacaaaaac ttaataataa ttttcagttt tatgtttagt ttttttagta
    13561 atatgtaata ttggataaat tatattgatt atttataaac taagtaataa aattaaaata
    13621 atatcacttt tttgtctatt atatataagg aaaaattttt taataatata aatcatattc
    13681 atttatatta ttaaaaaatt tgtttataga aacttttatt attttatata aaaatgataa
    13741 aaacttatat tttgtattta atagagtgtt gctaagtgaa tatatttaaa atggaggaaa
    13801 ctttatgaaa caaaataaat atgatgatga tagatttttt aataaataca gtaaaatgga
    13861 acgttctaca aatggattag caggtgctgg agaatggcat gttttaaaaa aaatgttacc
    13921 tgatttcaaa ggaaaaagag ttttagattt aggatgtgga tttggatggc attgtcaata
    13981 tgcagttgag aatggagcaa tttctgctgt tggagttgat atttcagaaa aaatgttaaa
    14041 agaagctaga aacaaaacgc aacttgataa tataaattac atatgtatgc ctattgaaga
    14101 tattaatttt ccaaaagata gttttgatgt agttattagt tccttggcat ttcattatat
    14161 tcaatcattt gaagatatat gtaagaatat aagtaattgt ctttcaaata aaggtaattt
    14221 tgtattttct gttgaacatc ctatatttac tgcatatgga agtcaagagt ggtactgtga
    14281 taaagatggc aataatctac actggccagt agatagatat tttgaagaag gagtacgtaa
    14341 gtcaaatttt ctaggagaag aggttataaa atatcataaa acattaacaa cttatttaaa
    14401 tacgttaatg aaaacaggat ttgaaatatt agaaattatt gaacctcaac cagaagaaaa
    14461 actgttagat acaattcctg atatgaaaga tgagctacgt agacctatga tgttacttgt
    14521 atctgcaaaa aaaagataag taaatataaa taatcattat tgtagaaaaa aattgtagaa
    14581 aataattaca ctaatggaga tgattatacc agtggtatta attacaccag tagtaaggag
    14641 gcaaaagcat tcgttgcttt tgccattttt taagttgcaa ttatacttct acgtagtata
    14701 tttcgtttcc attttcttct ataagctttt tcatatcttc aaattttaaa aatacagtag
    14761 cagtattatc atttggatgt atacctattt tatccatttt taataaatcc ttatcaaata
    14821 caacttttac aacagcctct ttatcattta atataccaaa aggagtcaca gctcctttag
    14881 taagtttcaa gtatttttga agtctttctt ctgatgcaaa actaagaggt ttactatcaa
    14941 tttgttgacg aatatctttt aaatctgctt ttttgtcttt acctaataca actaaaaaat
    15001 gttcttttcc tttagcatca cgaagaaata ggttttttac aacaaattcc atatgtaatg
    15061 tttttaattc ttccatttct tcaattgtat aaactggtgg atgatttaca gcctcataag
    15121 gtatgcttaa ctcatctaat tttttatata cttcctgttg ttgattgttc atgatttcct
    15181 cccattagaa atttatttta tatataaata atattatcac agaaaacttt aaaagaaaac
    15241 gttttatata atatagacaa tagattatat ttttataaag aagcaagaat actcaataaa
    15301 acttaaagaa attaaacaat ttctaaaaat accttgaaaa taga
//
LOCUS       DAEPOZ010000084        15290 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.084, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000084 DAEPOZ010000000
VERSION     DAEPOZ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15290
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.084"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..1762
                     /locus_tag="KR440_001888"
     CDS             <1..1762
                     /locus_tag="KR440_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HBH0698939.1"
                     /translation="VENIYNGLSKRNISVSIYHAGLSKNERSKNQEDFINDNVDIMVA
                     TNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFSPGDVHIQKY
                     LIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCNNCSNCLVEG
                     EIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLSTYNIMRDYS
                     SEDLKNFVNTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVKVEFKEIVMT
                     KSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKEEMLDISGVG
                     EIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDLKNVNSDFLEVNTDKVLYEKLLVI
                     REEYATKEKLPAYMILANKALKEISGRYPLDEEQLKDISGIGGVKFEKYGANILAEVN
                     KYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQEIEVSLSTII
                     GYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRKLPDSIKYEN
                     IRAVMLDSYLENLKNNIIAE"
     regulatory      1850..1961
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2103..3404
                     /gene="thiC"
                     /locus_tag="KR440_001889"
     CDS             2103..3404
                     /gene="thiC"
                     /locus_tag="KR440_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBH0698940.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            3458..3652
                     /gene="thiS"
                     /locus_tag="KR440_001890"
     CDS             3458..3652
                     /gene="thiS"
                     /locus_tag="KR440_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBH0698941.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            3645..4448
                     /gene="thiF"
                     /locus_tag="KR440_001891"
     CDS             3645..4448
                     /gene="thiF"
                     /locus_tag="KR440_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HBH0698942.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMPLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            4511..5281
                     /locus_tag="KR440_001892"
     CDS             4511..5281
                     /locus_tag="KR440_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBH0698943.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            5298..6401
                     /gene="thiH"
                     /locus_tag="KR440_001893"
     CDS             5298..6401
                     /gene="thiH"
                     /locus_tag="KR440_001893"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBH0698944.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEAISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYEFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            6402..7091
                     /locus_tag="KR440_001894"
     CDS             6402..7091
                     /locus_tag="KR440_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBH0698945.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKECRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYNEKLN"
     gene            7279..8673
                     /gene="yfcC"
                     /locus_tag="KR440_001895"
     CDS             7279..8673
                     /gene="yfcC"
                     /locus_tag="KR440_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBH0698946.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
     gene            9190..10557
                     /locus_tag="KR440_001896"
     CDS             9190..10557
                     /locus_tag="KR440_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBH0698947.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            10582..11190
                     /locus_tag="KR440_001897"
     CDS             10582..11190
                     /locus_tag="KR440_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HBH0698948.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGKNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMNDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYEKLTKV"
     gene            11238..11609
                     /locus_tag="KR440_001898"
     CDS             11238..11609
                     /locus_tag="KR440_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBH0698949.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            11788..12126
                     /locus_tag="KR440_001899"
     CDS             11788..12126
                     /locus_tag="KR440_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBH0698950.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            12593..13096
                     /locus_tag="KR440_001900"
     CDS             12593..13096
                     /locus_tag="KR440_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HBH0698951.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGAYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            13143..14102
                     /gene="moaA"
                     /locus_tag="KR440_001901"
     CDS             13143..14102
                     /gene="moaA"
                     /locus_tag="KR440_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBH0698952.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSVTRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            14106..14576
                     /gene="moaC"
                     /locus_tag="KR440_001902"
     CDS             14106..14576
                     /gene="moaC"
                     /locus_tag="KR440_001902"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBH0698953.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDYMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            14612..15043
                     /locus_tag="KR440_001903"
     CDS             14612..15043
                     /locus_tag="KR440_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HBH0698954.1"
                     /translation="MAKVVSINISEKKGVIKVPVPCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         6190 a         1409 c         2836 g         4855 t
ORIGIN      
        1 agttgaaaat atatataatg gtttatctaa gagaaatata tctgtatcaa tatatcatgc
       61 aggtttatct aaaaatgaaa gaagtaaaaa tcaagaagat tttataaatg ataatgtgga
      121 tataatggta gcaacaaatg catttggaat gggtattgat aaacctaata taagatgggt
      181 tatacattac aatatgcctc aaagtataga aaactactat caagaaatag gtagagctgg
      241 tagggatgga gaaaaaagtg agtgtgtact tttattttct ccaggagatg tacatataca
      301 aaaatattta attgatattg gagttgaaaa tcctgaaaga aaattatttc agcataagaa
      361 gcttcaatac atgattgact tagtttacag taattcatgc tatagaaaga caatattgag
      421 ttattttgga gaaaattata tagacaattg caataattgt agcaattgtt tagtagaggg
      481 tgaaatagta gataaaacta tagatgcaca aaaagtcatt tcatgtgtag ctagaatgaa
      541 aagaagttat ggtgttacta ctattgtaga tgtacttaga ggttctaaga ataaaaagat
      601 tttacagctt ggattaaata ctttgtctac atataacatt atgagagatt attctagtga
      661 agacttaaag aattttgtaa atacattggt ttctcatggt tttttagatt tagttgagac
      721 tctaggcaat ggaaatagag gtagttttcc tactataaga ttaaatgaga tgtctatgaa
      781 agttttaaaa ggtgaagtga aagtagagtt taaagaaatt gttatgacaa aatcattgga
      841 agtagaggat gagttatata atgctttaag agaacttaga cattcaattg caagtgaaga
      901 aaggatagct ccatatatgg tatttggaga tggtacactt agagccatgt ctagtagtta
      961 tccaattaat aaagaagaaa tgctagatat ctctggagta ggagaaatta aatatcaaaa
     1021 atatggtaga gattttgaag cagtaattaa agaatatgtc gaaaaaaata atataaataa
     1081 aaaaatagat gaaaaaaatg tcgatttaaa gaacgttaat agtgatttct tagaggttaa
     1141 tacagataaa gttttatatg aaaaactgtt agtcattaga gaagaatatg caacaaagga
     1201 aaaattacct gcatatatga ttttagccaa taaggctttg aaagaaataa gtggaagata
     1261 tccacttgat gaagaacaat taaaagatat ttctggcata ggtggagtaa aatttgaaaa
     1321 atatggagct aatattttgg cagaggttaa taaatatgta aaagaaaata atattaatgt
     1381 tatttgggag aaaaagggca aaaggaagtt aatattagat ggagaaagta gaaaaaataa
     1441 tgaaatagca ttagatttgc taaatcaagg aaaagatata caaagtatca gtcaagaaat
     1501 agaagtttca ttgtcaacca ttattggata tgtgcatgac tatgtaaagg atggacatag
     1561 tatatcattt gaattaggac tagaaaattt ttataatgaa gataaaaaga agatgatttt
     1621 agaagtttgc gaaaaagttg gctatgataa tttaaataac ataaaaagaa aacttccaga
     1681 ttcaataaag tatgaaaata taagagctgt gatgcttgac tcttatcttg aaaacttaaa
     1741 aaataatatt atagcagagt aatttttaac ttaataaaaa taaaatttgt attattgtgt
     1801 tgaactaata attttgctgt gttatactga atttgtaaaa ataatgaaaa attagagcta
     1861 ggggcgctag aattggctag ctgagagata aagccgtaac ttacaaaccc taataacctg
     1921 atcaagataa tgcttgcgaa gggaagcttt ttaatataat tttattatat aaataggcaa
     1981 attattcaat gttaggagaa atttgtctaa gtgtatatac gattataaga gtgctttcca
     2041 aacgaggaaa agcacttttt ttattttcaa aaactataac tttaatcttg gaggattgta
     2101 atatgaacta tacaacacaa atggatgctg ctagaaaagg cataataact aaagaaatgg
     2161 aaatcgtttc tcaaaaagaa caggtagatg taaatgaatt aagagaatta atagcaaatg
     2221 gacaagttgt tataccagct aataaaaatc ataaatcttt aagtgcagaa ggtgtaggta
     2281 aaaatttaag aacaaaaata aatgtaaatc taggaatttc aagagattgt aaggatatag
     2341 aaaaagaact tgaaaaagtt agagtagcaa tagacatgaa agctgaagct ataatggatt
     2401 taagtaatta tggcaaaact agagaattta gagaaaaggt tgttgaaatg tcacctgcaa
     2461 tgataggttc agtacctatg tatgatgcag taggatattt ggaaaaagaa ttaaaagata
     2521 taacagaaga agagttttta aatgtaataa gacaacatgc tattgatgga gtagacttta
     2581 ttactataca tgcaggtctt acaagaagtg tttgccaaaa aataaaaaat catgagagat
     2641 tgactcatat agtatctcgt ggaggttctt tattgtttgc atggatggaa ttaaataata
     2701 aggaaaatcc tatttatact aattttgata aaatattgga tatatgtgaa gaatatgatg
     2761 ttacattgag tttaggagat gcttgtagac caggttgtat aaaagattct actgatggtg
     2821 ttcaaattca agaacttgta gtattaggtg aacttactaa aagagcatgg gaaagaaatg
     2881 tacaagtaat gatagaaggt cctggacaca tggctattga tgaaatagaa gcaaatgtag
     2941 tattagaaaa gagattatgc catggagcac ctttttatgt tcttggacca ttggttacag
     3001 atatagcacc aggttatgac catataacaa gtgcaatagg aggagcttta gcatgtgcta
     3061 aaggagtaga ttttttatgt tatgtaactc cagcagaaca tttaagattg cctaatttag
     3121 atgatatgaa agagggtata atagctgcaa agatagctgc tcatgcagga gatatagcta
     3181 aaaatgttaa aggtgctcgt gaatgggata ataagatgag taaagctaga gctgatttag
     3241 actggtgtga gatgtttaga cttgcaatag accctgaaaa agctaagaga tacagagatg
     3301 aatcaactcc tactcatgag gatagttgta ctatgtgtgg aaaaatgtgc tcaatgagaa
     3361 cagttaagaa aatattaaac aatgaggaac ttaatctaat ataatttttg gctagtatga
     3421 ctggaataaa aagaagctga aaagaggagg tgatttagtg aaggttaatg ggaaggaaat
     3481 tgaatttgaa aaagatttga ctgttataga tttattaaat aaatataatt taaaaagtga
     3541 tagagttgta gttgaagtga atttagagat aattgaagaa tctaactaca atacatatgt
     3601 attaaaagac gaggacattg tagagcttat tagctttatt ggaggtggtt aaaataaaaa
     3661 tatttgtaaa tgaattatta acagatgttg aggaaaatac aactgcttat aaagttagag
     3721 atagctataa caataaatgt gatgtattgg tgttaaatgg atatccaatt aaaacagata
     3781 tgcccctttg tgaaaatgat aaattaacac ttatacaaaa aggagttaag ccatcacttg
     3841 atgaattgga aagcttgttg attgctagac atactccaaa tatacataat aaattaaaaa
     3901 aagggaaagt tgctatattg ggattggggg gacttggttc caatatagct atctctttag
     3961 ctagaatagg agtgggagaa cttttactta tagattatga tgttgtagaa ccatctaatt
     4021 taaatagaca acagtatttt atagatgata taggaaaact aaaaactgat gccatgatag
     4081 aaaatataaa aaaaataaat ccatttataa aattaaataa acgagatatt tttttaaaca
     4141 agaacaatat ggatactatt aaggattcag atttaataat tgaggccttt gatgatgctt
     4201 catgtaaagc acaggtatgt aattatgtat taataaattt aaaagataaa tatttaatag
     4261 catcatctgg tatggcaggt tactatgatt caaatataat aactactaag aagataaaag
     4321 acaggtttta tatatgtggg gattttgtga atgaagctaa atttggtgaa gggcttatgg
     4381 ctccaagggt tgccatctgc gcaaatcata tggcaaattt ggcaacgcaa atacttatag
     4441 atatgtaaat attaaaatta gatataaaaa agattgaata ttaaaaaaat ttaacgtgga
     4501 gggaaataat atggataaat tggtacttgg tggacatgaa tttaatagca gattattggt
     4561 tggaacaggt aaatatggtt caaataatat attgcctgag gtaataaaag aaagtggaag
     4621 tgaaataata acaatggcac ttagaagagt ggatttagac aataaacaag aaaacatatt
     4681 aacgtatatt ccaaaagaga tgacaatact tccaaataca tcaggagcta ctaatgcaga
     4741 agaagctgta agaatagcta gaatatctag aaaaatggga tgtggagatt ttataaaaat
     4801 agaagtaata tcagatacta gatatttact tcctgataat gaagaaacta taaaagctac
     4861 aaagatactt gctgatgagg ggtttatagt tcttccatat atgacaccag acctttatgc
     4921 tggaagaaga cttatagaag ctaatgcagc cgcagttatg ccacttggag ctccaattgg
     4981 ttcaaataga ggtcttcaaa tgaaagaaat gataagaata atgatagatg agttggatat
     5041 accaataata gtagatgcag gtattggaaa accatctcaa gctatggagg ctatggaaat
     5101 gggagctgat gcagtacttg tcaacactgc tatagcttca gcaggagacc ctgtacaaat
     5161 ggctagagca tttaaattag ctgtagaagg tggaagagaa gcatatattg caaaaacagg
     5221 aaatgtgtca gaatttgcaa atgcatcttc tcctttaaca ggttttttag gcaatttata
     5281 gaaagtggtg attggtgatg agtttttatg atgtaataga aaaatatagg gattttgatt
     5341 ttgatggata tttaaataat gttactgata atgatgtttt aagaagttta tcaaaagaca
     5401 agcttgaaga ttttgatata ttaaatttgc tttctaaaac tgcagtaaaa catttagaag
     5461 atatggcaca aaaagcacat aaattaagtg ttcaatactt tggtaaaaca gtatgtttat
     5521 atacaccaat gtatatagca aattattgtg ttaatcaatg tgtttattgt agctataata
     5581 taaagagtgg aataaaaaga aaaaaattaa ctatggatga aattagagaa gagggagaag
     5641 caatttcaaa agaaggtttc aaacatctac ttgttttgac tggagaaagt agttttcact
     5701 caagtgtaga atatataggt gaggctatag aaatattaag agagaaattt ccctctatag
     5761 gtatagaagt ttatccaatg gaagtagaag aatataagta cattgtagat aaaggtgttg
     5821 aaggattaac agtatatcaa gaaacttatg acgaggaaat atataagaga gtacatataa
     5881 aaggcccaaa atcaaattat gaatttagac tagatgctcc tgaaagaggg gcaaaagcag
     5941 gtatgaggac tttatcaata ggtgcacttt taggtctaaa tgattttaga aaagaaactt
     6001 tttttacaat attgcatggg aaatacttaa aaacgaaata tcctcatatt gagctttcat
     6061 attcaacacc aagaatgaga ccttttaaag ggtgttttga ggaattggta gatataagtg
     6121 atactgattt agttcaagct atggtatgta tgagattatt tgaccctcat gcagctataa
     6181 atatatccac tagagaaaat ttagaaatga gaagtcatat aataccttta ggagtaacta
     6241 aattgagcgc aggtgtatct actgatgtag gaggtcattc tcaagatgaa catgatactg
     6301 ctcaatttaa gataaatgat gaaagtactg ttaaagatgt ggagaaaatg ctaaactcaa
     6361 ttggttatca gcatgtgttt aaagattggg aaagatttta aatgtatctg ataacaaaca
     6421 gaaaattatg tagtgaggaa cgatatttag aagttattaa agagtctata ttgagtggtg
     6481 tagaaaatat aataataaga gaaaaggatt tggaatatca agagctaaga aaattatata
     6541 tgaaaataaa aactaaaatc aattgtatag attttcaaga gcaaatatca gatgaaagtt
     6601 tgaaaactaa tataaatcaa aaagagtgta gaaataaatt caaggttaat tttataataa
     6661 atagtaacat tgaatttttt gaaaaagtgg attgtcaagg tatccatctt ccttttaaat
     6721 tattcttgaa tttaattgaa aataagtata attttaatga aaataaaata ctaggattat
     6781 ctttacataa agtagaggaa gtagattatt tagaaaaatt aataagaaat caaaatataa
     6841 aaatagatta cataacatta tcacatatat atgaaactaa atgtaaagaa ggattaaatc
     6901 caaaaggaat tgagctttta aaagaagcta aaaagattac tgatattaaa ataattgcac
     6961 ttgggggaat tttaccatct aatgtcaaag aaacattaaa atactgcgat gattttgcta
     7021 taatgtcaac tataatgagg tctaaagata ttaaaaaaac catatcaaat tataatgaaa
     7081 agttgaatta gtaaatatag tatattgaat ttttaactta aataaaaata aaaaagttat
     7141 cttttaaatt ttaaaaggta gcttttttat tttatatctt tgctaataaa aaattgtaaa
     7201 aagggaaaac tttagtcaga gagattaaac aatatcctag aaagatttaa aatttagtaa
     7261 agtatggagg acattataat gactgacagt aaaaagaaac gatttattat accacacact
     7321 tttacaataa tatttttact aatagtcttg atggcaatat tgacttggat agtaccaagt
     7381 ggtgaattta gtagagaaga tgtagaaggt agaatggtag ttgtaccagg gacttatcaa
     7441 agtgtagaaa gtaatcctca aggatttgca gatgttttta aagcaccaat agaaggcttt
     7501 atagattcag ctgaagttgt aggttttgtt ttaatagttg gaggtgcttt tggtattgta
     7561 aataggactg gagctataga agcaggaata gcttcggtta tctctaagtt taagggaaaa
     7621 gaacttctta taattccaat atcaatgatt ttatttggac ttggaggtac aacatttggg
     7681 atggcagaag agacattacc attttatatg atatttgttc cactcatgac atccttggga
     7741 tatgattctc taacagcaat agcaattgta tttataggag cagcagcagg agccgccgct
     7801 tcaacaataa atccattttc agttggtatt gcacaggcac tagctcaact tgctcctggt
     7861 tcaggagtaa cttacagagt agttattttt atagcatata tactaataag tatagcattt
     7921 gttatgtact atgctaagaa ggtaaaggaa aatcctgaaa gttcaatagt atatgaaata
     7981 gataaaaata ataaatcaat acttaatcaa acttcaacag aaataaaaga atttactttt
     8041 aaagaagtat ctgttcttgc tatatttgga atagggatgg caataatgat ttggggtgtt
     8101 ttaagtcaag gatggtacat tccagaaata tcaatgtgtt ttgctgcaat aggtgtgctt
     8161 tctggattgg ttggtagatt atctcaaatt gaaatctcag aaagttttat agatggagca
     8221 aaagatttag catctgcagc tcttgctata gctttggctc gtggaatcgt aataattgct
     8281 caaaatggat ttataataga tactatatta aattccgcag cagcctttct aggtgggctt
     8341 ccaaaggtta tatttgtata tttatcattt ttcttggaag ctgcattggc atttttaatt
     8401 ccatcatctt caggtcttgc ttcacttaca gtacctgtac ttgctcctct tggtgatttg
     8461 gttaatatat cggctcaagt gatagtaacc gcttatcaat ttggtgtggg tcttacaaac
     8521 tttataactc caacttcagg agtactgatg ggagcacttg cagtagccca tattccattt
     8581 tcaaaatggg taagatttat aatgcctatc ttgttgattt tgacagctgt atctctagtc
     8641 tttttaacaa taggtattta tatagggttt taattaagtg tagttttatt taagatattt
     8701 agtaatggta taaaatgata atatgttttc aataaattgc cactaattta attagtggta
     8761 atttattttt tgtatttaaa aataaatgta tttacttgta ttttgaatta atatctactt
     8821 agacattgtt ttaattgtta tattttcatt taattaagca ttgatttatc gttttttata
     8881 tttcatataa caatgcaaaa aataattttt atatagtaaa tgacaaaagt ttacaataaa
     8941 tgtatatttt cttactcatt ccttaaaatt actcatttag tttaataact ctcatttcat
     9001 atgtaaagtt ggtaaaaaaa ttaaaattaa aagtaaaaaa ctaaataaat taatgttaat
     9061 ttgaaatata tatgaaggtt tttggagaat acttacttat gataggaaaa aatgttaaaa
     9121 taatagtgta atcaaatata aaggactgaa ataattaaca gttattaaat attaggagtg
     9181 taaaaattta tgggattaaa agaatttgat tttattgaag agtctattga tatgttgagg
     9241 acaatgtcac caacattaga aactatatca gatgaaatag aagagtattt tgaaaatatc
     9301 ttggatgaaa agaatcaaga atatataaat gtaacctcta gaataaaatc agaatcaagt
     9361 ttaagagaaa agataattag aaatagatat ttaaaaaaat atggagaagc aagtaatctt
     9421 attcataatg tttctgactt aataggccta agaattgagt gtagatttat tgaggatgaa
     9481 aacaaaatat atagactttt gagaagatat tttaataaaa cagatgacaa aataaactat
     9541 tataataaag agaataagaa tattaaatta aaactatctg agaggcaacc caataagcaa
     9601 aaaaatggat ttgaaatata taaaatagat ggagtttttg cgtatttaga tagagaagta
     9661 aaatttgaac ttcaaattaa atctcttgta aatgtgtttt ggagtgaaat agaacataaa
     9721 ataatttata aaaataatac atatttactt gctgataaat ttataaagga tatgatggac
     9781 tctataaaga ataatcttac tatgatagat aatcagcttt taagtattta taaaaacttt
     9841 cattcaggga agtcttttaa tatgaaagta agtaaaaaag aaattgaaaa attatttgcg
     9901 aaactagtct atgacgcttt ttctgaaaag atgaataaaa gtataggttt tgttgtagat
     9961 tttaaaaaac catgtgaaac tatattgaac tattctttta ataaacaaga tataagtgat
    10021 gaagttttag gtaattttat gcttgatgag tttgcaagat taaatgaaat tgtaaacaag
    10081 gatatagatt ttaatgagca aatagaattt gaaagagaac caacatttga tgataagttt
    10141 tgtaaagacc taggaaatca ttttagaagt agattaaaca cagaatttcc atggaaccta
    10201 ttttttagaa tattatttga aatagagcca tataacaata ccaaagactt tgaaaacttt
    10261 gtagagttta taaaagaaaa tgtgctacta gaagaaaata gggagcaatt attatgccaa
    10321 tttgaagaag atagcaagct tataattgaa gatatatatg attgtatatt taatagtata
    10381 agtgagattg atagtgttga aattttatat aactataatc ttgaaaaaat aaactttaca
    10441 tcaagtgaaa tcataaattg tatatgtaga gaatatgagt gttatggtga atacttagaa
    10501 gaaaaagaaa agcttatgaa tacatttaaa gaaaagatta ttcaaatctt tgagtaatag
    10561 gtcaatgctg aagtggcgct aatgcgccat ttttcaaata tatatgaatt aagtaaaatt
    10621 attttggagg aggcgatttg tatagagaaa acaggtgtaa ttttggcagg gggaaaaaat
    10681 tctagaatgg gtagagataa agccttttta gagttacatg ataagctttt tattgaaatt
    10741 gcaatagaag catttaaaaa ctttgatgag ttgataataa tatctaataa tgaagaatta
    10801 tattctaaat acgatattaa agtttataat gatatagtaa aagatgttgg tccaattgga
    10861 ggaatatata ctgctcttga atatgcaaag tatgatatag ttacaattgc atgtgatatg
    10921 ccatatctaa ataatcaaat agtcgaaaga atagctaata aaatgaatga taaaagtgtg
    10981 atttcagtga caaatggaaa gttacaacca ttgtgttctg gatataaaaa gagtataata
    11041 aataaagtat ctttatgtat aagggaaaat gatttaaagc tcagaagctt tatagataaa
    11101 atagataaaa gttatatata ctttgatgaa gaagatttat ttttaaatgt aaacactgtt
    11161 gatgagtatg aaaaattaac taaagtttaa ttagctatgg ttgtaaaata gagtaggatt
    11221 tatatggagg agagtttatg aatttactta aatgcgattc ttgggcagta atattaaata
    11281 atagcgataa agaaagtaaa gcatataaaa tattagatga acttaaaagg aatatgtaca
    11341 aagttgttgc tatagatgaa gagaaaaaac ctattgaagg aatagatgtg tatgaatgtt
    11401 taaaagatgt tccacacaat attgatgttg tagctattat tgataaacaa tctaagatgg
    11461 atgtgatttt agaagaagta gagcttttgg atatacaaaa tatgtggttt gaaaaaggaa
    11521 gctttagtga aatgatgatt aaaaagacta aagatttaaa attaaatata gaatataatt
    11581 taagtttata tgatgagttg actagataaa attatatttc ttaataataa aaatataatt
    11641 agttaataaa acatattatt ttatagtata gattggttaa taaaagtaca gattggttaa
    11701 tgaaacatta aattacaata tttgttaaaa gaattgtgag ttacatgata taatgtatta
    11761 aataggatac taaggagttg atatattatg ttaatagtaa ctacagaaaa agtagaaggt
    11821 aaaaagatat caaaggtttt aggattagtg agaggaagta caataagagc aaaacatgtt
    11881 ggaaaagata taggagcaag ttttaagaat cttgttggag gagaacttac tggatataat
    11941 gaaatgctca ctgaagcaag acaaattgcc ataggtagaa tggttgaaga tgcagaagct
    12001 aaaggtgcaa acgcagtaat agcatttaga ctgtcttcag cttcagttat gcaaggggca
    12061 gcagaaatgc ttgcttatgg aacagcagtt gttttagaag atgataatag tattcttgaa
    12121 aaataaaata taaatagtta aaaagaggca tcttttaaaa attttatgga tgcctttttt
    12181 tatttgaaaa aactaatttg agaagaaatt ttaactatta tgacaataaa attagggtga
    12241 tattatataa ataagtaaaa ttatattata taatgtatgg tatagacttt aaaaatttta
    12301 tttgtagaga aaaatatgga caatgttata ataattatag aaattaaaag tacttacatt
    12361 cattatgtat gtgacatgta taattagttt tatatttaat acagttttga agtaatatct
    12421 tcattttgag aacgttggaa taataaaaaa tgtatgtatt ataggtgata taaataaaaa
    12481 ataaatataa tatgttatat tgctgataga aaagagaata aaagaaaata ggttttatta
    12541 aaagagattt ttatttatag gacttaatca atgtatagag gggtgagaac atatgtttaa
    12601 tgttgcaatt ataacactta gtgataaagg atatgaagga aaaagagaag atataacagg
    12661 aaaaaagtta actgaatttg ttgaaaatac aggagcttat aaagttactg agtatgtact
    12721 tataaaagat gataaggaaa tgctaaagga aaatattata agactttgta atagtaataa
    12781 aatagattta attcttacaa atggaggtac tggtttttca aaaagagata taaccccaga
    12841 agcaactaag gaagtgttgg aaaaagaaat accaggtctg agcgaatata tgagaatgaa
    12901 atccacagaa attactaaaa aggccatact aagtaggggt gtgagtggaa taagaaataa
    12961 ttctataata ataaatttac ctggaagtcc taaaggtgca gttgaaaatc taagttttat
    13021 aattgatgtt ttagaccatg gcatagaagt tttaagagga gaagctacag aatgtgccac
    13081 taaaaaaagt gaatagtata taatctagat agataaaaaa tagacaaaga ggtagataaa
    13141 gtatgattga taagtatgga agaaaaatag actaccttcg tatttcactt acagataagt
    13201 gtaatttaag gtgtgtctat tgtatgccac cagatgtaaa atttgataaa aattatataa
    13261 atgaaaatct aagctttgaa gactacaagt ttattataaa ggctatggct gaacaaggta
    13321 taacaaaaat tagatttact ggaggagaac ctcttttata tcctaaatta aatgagctta
    13381 taaaatttac aaaagaagaa tgtggaataa ataacatagg aatgactact aatgctattg
    13441 ggctttctga tagaattttt gagcttgaaa aatgtggctt aaataaagtt aatataagtt
    13501 tagattcttt gaaggaatat aagtataaat ccgtaacaag aggaggaaac ttaaaagatg
    13561 tcttaaaatc gattgaatct tgtttaaact taggtataaa agtaaagtta aattgcgttg
    13621 ctataagtgg gtttaatgat gatgagctta aagattttat gtttttaaca gttaaatcac
    13681 caattgatgt aagatttata gagcttatgc cattaggtga ggctaataga gtttataaga
    13741 agggttattt taatgtaaaa gaaatgattg aaaaaatata tggtttatat aaagtcaaaa
    13801 atgatgaaaa aagtacagca gaatattata tgtttaaagg agctaaagga agaataggaa
    13861 ttataactcc attaagctgt tcattttgta acacttgtaa tcgtattaga cttacttcta
    13921 gtggtgcaat aaaattatgt ctacattcaa aagaagaaat agatataaaa ccatttttac
    13981 ataaaccatt gttatttaga gaatctatga aagatataat aaaacaaaaa ccagaaaaac
    14041 atcatttaat agaaaataaa tgtagtgata caaataggcg tatgtatgaa attggaggat
    14101 aagtaatgtt aacacatata aatgaaaaag ggtatgccaa gatggtggac ataagtgaaa
    14161 aagatgatac taaaagaacg gccgtagcta cagctacaat tactcttagt aaggaagcat
    14221 tagaaaaagt aaaaaatggt cagataaaaa aaggagacgt attgtctgta gctcaagttg
    14281 caggtataat gggagctaaa aatacatctt caaacatacc aatgtgccat cagattaaat
    14341 tagttggatg tgatttagaa tttgatgtag atgattatat gtctgctatt aagatatacg
    14401 caacctgtaa atctcttggg aaaactggag tagaaatgga agctttaaat gcatgttctt
    14461 tagcagcact tacaatttat gatatgtgta aagcagttga taaaaaaatg gttataagta
    14521 atatccatct tgtggagaag actggtggaa aatcaggaga ttttaagttc gagtaaatta
    14581 ataatatatt gggttaaaat ttggaggaaa tatggcgaag gtagtgtcaa taaatataag
    14641 tgagaaaaag ggtgtaatta aagttcctgt accatgtgca gagctaaaaa taaatcatgg
    14701 gatagttgga gatgcacatg caggaaactg gcatcgtcag ataagcttac ttggaaaaga
    14761 aagcatagat aaaatgaaaa tgaaaggctt tgaccaatta aaatatggag attttgctga
    14821 aaatgtaact acagaaggga tagaagtttt ttccctgcct gttggtacaa agttaagaat
    14881 agggcaatgt gaagttgaag taactcaaat agggaaaaaa tgtcacaatg gatgtgaaat
    14941 taagaaattg acaggtgact gtgtgatgcc acgagaagga atttttgtaa aggttctaaa
    15001 agaaggaatt ataaatgtga atgattcaat agatgtaatc taatgaagtc actattaaaa
    15061 tataattata agtaaatact attaagattt aaatatatat tttaagagag aattaattta
    15121 aataaaatat tttagttata aattggaata ttggttatat aaagctatga atgtaaaatc
    15181 atagcttttt gtttttgaat tttaaaatat aatattttat ttatatatat taaattatat
    15241 tttatataaa atcaattgtt taaatatata ttatttatac tgtaaattta
//
LOCUS       DAEPOZ010000085        15282 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.085, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000085 DAEPOZ010000000
VERSION     DAEPOZ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15282
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.085"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            234..2039
                     /gene="lepA"
                     /locus_tag="KR440_001904"
     CDS             234..2039
                     /gene="lepA"
                     /locus_tag="KR440_001904"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBH0698955.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDERRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            2190..3527
                     /locus_tag="KR440_001905"
     CDS             2190..3527
                     /locus_tag="KR440_001905"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBH0698956.1"
                     /translation="MINIEIPKKVDYIIKELETNGYEAYVVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLISTEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILRRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGHILNKMLEIVINNPELNEKEILKEKALNIYTF"
     gene            3760..5073
                     /locus_tag="KR440_001906"
     CDS             3760..5073
                     /locus_tag="KR440_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH0698957.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTIISLGGIFITLLGLPVYYYMKKR"
     gene            5245..6420
                     /locus_tag="KR440_001907"
     CDS             5245..6420
                     /locus_tag="KR440_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBH0698958.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMKLY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINVDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            6523..7542
                     /gene="hrcA"
                     /locus_tag="KR440_001908"
     CDS             6523..7542
                     /gene="hrcA"
                     /locus_tag="KR440_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HBH0698959.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            7572..8192
                     /gene="grpE"
                     /locus_tag="KR440_001909"
     CDS             7572..8192
                     /gene="grpE"
                     /locus_tag="KR440_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBH0698960.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            8284..10131
                     /gene="dnaK"
                     /locus_tag="KR440_001910"
     CDS             8284..10131
                     /gene="dnaK"
                     /locus_tag="KR440_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBH0698961.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLADEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            10342..11496
                     /gene="dnaJ"
                     /locus_tag="KR440_001911"
     CDS             10342..11496
                     /gene="dnaJ"
                     /locus_tag="KR440_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBH0698962.1"
                     /translation="MSTKRDYYEVLGISKDAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            12462..13397
                     /locus_tag="KR440_001912"
     CDS             12462..13397
                     /locus_tag="KR440_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBH0698963.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     AEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKDYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EDLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            13433..14806
                     /locus_tag="KR440_001913"
     CDS             13433..14806
                     /locus_tag="KR440_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0698964.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEGNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(14994..>15282)
                     /locus_tag="KR440_001914"
     CDS             complement(14994..>15282)
                     /locus_tag="KR440_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0698965.1"
                     /translation="RYKTLDLINEDNILNKLIDFNKIKSIDEELLYTGIKFINNDLNI
                     SKTSTSMFLHRNTLVYRLEKINEILGFDLKNFENAMIFYLSVKSYFLYKKI"
BASE COUNT         6030 a         1663 c         2795 g         4794 t
ORIGIN      
        1 tatattttta atgtataaaa taaaaattca ttaactgtat tttataggat ttactttaag
       61 tttacgatat tatataatat ttagtgttag acttgataat ccacatataa tgtggataat
      121 tttgtttagt gccaattagt ttatattcaa aaattttaaa tttggtatat aaagagtttt
      181 tagataatgt aaaataataa ctataagaat aatcaagagg agggaatttt aaggtggaca
      241 ataaacaaag tagaactaga aattttagta taattgcaca tatagaccat ggaaagtcta
      301 ccttagctga tagattaata caacaaactg ggcttgttag tgaaagagac atgaaaagtc
      361 aattacttga caatatggac cttgaaagag aaagaggaat cactataaag cttcaaaaca
      421 taagattaat gtataaagcg aaggatggaa atgaatatta tttaaatctt atagatacac
      481 ctggacatgt agactttaac tatgaggtat caagaagttt agctgcatgt gaaggagcac
      541 tattagtagt tgatgcagct caaggtgttg aggcacaaac tttagccaat gtttatcttg
      601 ctatagacca agatttagaa atattaccaa taataaataa aatagattta ccaagtgcaa
      661 gaccagagga agtaaaaaat gaaatagaag atttaatagg gcttgattca agtgaagctc
      721 ctcttatttc agctaaaact ggtttaaaca tagaagatgt tttagaagat attgtaaaaa
      781 atgttcctcc acctaaggga gataatgaag ctccattaaa ggcattaata ttcgattctt
      841 actatgatgc ttacaaaggt gttgtagctt atgttagagt atttgaagga actgttaaaa
      901 agggtatgac aataaaaatg atgaatacca ataaaaagtt tgaggtaact gaagttggtg
      961 ttatggctcc aggtcaaact gaacttagtg aattatcagc aggtgatgtt ggttatattg
     1021 ctgctagtat aaaagatata agaagttgtc gtgttggtga tacaataaca gattcaaata
     1081 atcctacaga agaaccttta ccaggataca aaaaagctac tccaatggta tattgtggta
     1141 tatatccagg agaaggagaa aagtatgaga atgttagaga tgctcttgaa aagcttcaag
     1201 taaatgatgc tgcacttgaa tatgaagctg agacttcagc agcactagga tttggtttta
     1261 gatgtggttt cttaggtctt ttacatatgg agataatgca agaaagactt gagagagaat
     1321 ttaaccttga tataattact acagctccat ctgttatata tagagttaca aaaatggatg
     1381 gagaggttgt aatgatacaa aatcctgcaa acttaccaga accatctgag ataaagatga
     1441 tagaagagcc tatagtaaaa ggagatataa tagttcctaa agactatgtg ggtgttgtta
     1501 tggagctttg ccaggaaaga cgtggaaata tgttaaatat ggaatatata gatgagagaa
     1561 gagtaatgct tcactatgac cttcctctca atgaagttgt ttacgatttc tttgatgctt
     1621 taaaatcgag aactagaggt tatggttctc ttgattatga ggttaaaggt tatgtagcat
     1681 ctacacttgt aaaacttgat atattaatca ataaagaaca agtggatgca cttagtttta
     1741 ttgtccatga gacacgtgct ttccctagag gaaaggcaat gtgtgagaaa ttaaaaggag
     1801 aaattccaag acatcagttt gctataccta tacaagctgc tgttggtaat aaggttattg
     1861 caagagaaac aataagcgct ctaagaaaag atgttcttgc taaatgttat ggtggggata
     1921 tatctcgtaa gaagaaactt cttgaaaagc aaaaagaagg taagaagcgt atgagacaaa
     1981 ttggttctgt tgaagttcct caaaaagcat ttatgtctgt acttaaacta gatgaataaa
     2041 ttcttataaa tttagataaa tatatttaaa agctagagtg attttagaat tatttctaaa
     2101 ttaatctagc ttttttgtgg tatattttaa gtaaactcat agagaatata agtaagaata
     2161 tattaaagaa gaataaaaag gtgatattta tgattaatat agaaattcct aaaaaagtag
     2221 actatataat taaagaatta gaaacgaatg gttatgaagc ttatgtagtt ggaggatgcg
     2281 ttagagattg tttattagaa agagttccaa acgattggga tataacaact agtgctagac
     2341 cagaagtggt agtagaatta tttgaaaaaa caattccaac tggtatacaa catgggactg
     2401 taacagttat gattgaacat gaaccatttg aagtgacaac ttatagaatt gatggaaatt
     2461 atagtgatgg tagacatcca gattcaatag aatttacaaa taacatagtt aaggacttgt
     2521 caagaagaga ttttactatc aattcaattg catacaattc aaagacagga ttagtcgacc
     2581 catttaatgg ctatgaagat atacaaaata agtatatacg ttgtgttgga aatccagtag
     2641 atagatttga agaagatgca cttagaatgc ttagggcagt tagattttca gcacaactta
     2701 attttaaaat agctgaaggt actaagcaaa gtattcataa aaaagcagat ttgataaaaa
     2761 atgtttctat cgaaagaatc cagacagagt ttaataaaat attagtatct gattcatcta
     2821 aattaaactt attaaaatca acaggtttgt tgaaatttat aattcctgaa atatgtgaat
     2881 tagaagatgt tactcaacac aatccatatc acatctatga tgtacaaaaa catactttaa
     2941 tatctacaga agtaattgaa gatgaactgt atttaaaact tacaatgttg tttcatgatt
     3001 taggtaaaaa agtaactaaa acaactgata aaaatggtgt tgaccatttt tatactcata
     3061 gtagagaatc tgtaaagatt gcaaagaaga ttttgagaag acttaagtat gataattata
     3121 ctattaataa agttttaata ttaatacaat atcatgatta tagaatagaa cctaaaagga
     3181 agataattaa aaagttatta aataaacttg aagatgttga attatttgaa gatttaataa
     3241 aagttaattg ggctgataca cttgccaaaa acccaaagta tgcaaaacaa aagatattaa
     3301 atcttattga gtgtgaaaaa gaatttaaac atataataaa tcaaaaggag tgctttaatc
     3361 ttaaagattt agctattaat ggaaaagatt tgatatctat aggtataaaa ccaggtaaag
     3421 atataggaca tattttaaat aagatgcttg aaatagtcat aaataatcca gaattaaatg
     3481 aaaaagaaat actaaaagaa aaagcattaa atatatatac attttagatt caaatataaa
     3541 aaatttaatt actttaaaaa attatagaag aaaatactta tttagataaa ataaataatt
     3601 tttagagaat attttagaag ttacgattgt atgattttta aacgcatagc atttactcta
     3661 ggactataga ctaatataaa aaaaaacttt gggaatacta aaaccataaa tttataaaat
     3721 taatatttta tatactgtaa aacagtgagg agggacagta tgcatactca actacaaaag
     3781 actattggtc tttcagcggc tctttctact gttgtaggta tggttatagg ttcgggagtt
     3841 ttttttaagc cacaagcaat atacactaca actaatggag ctccaggtct aggtattata
     3901 gcgtggcttt taggtggttt tataacaata actgctgggc ttacagctac agaaatttca
     3961 gctgctatac caaaaactgg tggtatgatg atttatatcg aagaaatata tggagaaaag
     4021 ttaggctttt taactggatg gatgcaaact gtattatttt tcccaggtac atcagcagca
     4081 ttaggagtaa tatttgctca acaagcatct gaacttttag gaatgagccc aaataatatg
     4141 gcaaatgtac ttcctatagc aattggagtt atactatttt tagcgttatt aaatataata
     4201 ggttcatcct taggaggaaa agttcagaca gtagccacta ttggaaagat gataccttta
     4261 attttaataa tagtctttgg atttattaaa ggtcaaagta gtgaagtatt aaatccattt
     4321 gtaggagatg gtgtaaatgc atcaaatgca cttggacaag tattaatagc tactttgttt
     4381 gcatatgatg gttggataaa tgttggagct atatcaggag aaatgaagtc tccagagaaa
     4441 gatttaccaa gagcaatagt tggtggttta tcattagtta tggcagttta tattataata
     4501 aatatagctt atctttgggt agtaccagca agtgaattag caacagttac ttccccagct
     4561 acattagtag caacaagatt gtttggaaat ataggtggaa aagtaataac tgtgggtata
     4621 ttaatatcag tatttgggac attgaatgga tatttattaa caggttcaag gataccttat
     4681 acattagctg aaatgggaac tttaccagca tctaaaactc ttttaaaagt aaattctgga
     4741 ggttctcctg taaattcaat actacttata actgtgttag cctgtgtata tgcattatct
     4801 ggtcaattta atcttttgac agacttaact atattttcaa tatgggtatt ttatgtattg
     4861 acttttatag gtgttatgag gcttagaaga caaaagccag atttacacag accatataaa
     4921 gtccctttat atccaataat accaattata gctatactag gtggactgtt tgtaataata
     4981 aatcaaatat tgacttcaac aattatatct ttaggtggaa tatttataac actattaggt
     5041 ttaccagttt attattatat gaaaaaaaga taactactta atttaagtta gataaatttg
     5101 ggtaaaggtg aaaattaact tttatccaaa ttttttatac tgttatacaa tataagcatt
     5161 atatattact tgatgtgtta ttttgtttga tttataattt agtaattgat gctttaagat
     5221 aatttaagaa atgaggaata aaaaatgcta ggactttatg tacatattcc attttgtgtg
     5281 aaaaaatgta agtattgtga ttttaattct tataaaatgg acatagattc aaaaaaaaga
     5341 tatatagaag acttaaaaat agaaatgaaa ttatatagta ataaattata taaagataat
     5401 aaatataaaa ataaagaatg ctgtagtcta aataaaaatg acaaaataac gagtatattt
     5461 gtagggggag gtacacctag tatactaaca tctgatgaaa taagagaagt atttataagt
     5521 ataaaagaaa tgtttgatat agatgagaat gcagaaataa ctatagagtg taatcctggg
     5581 acattaacat tagaaaaatt aaaaactatg aaagagattg gcataaatag acttagtata
     5641 gggctacagg ccattcaaga aaagcatttg aattttatag gaagaataca tacttatgaa
     5701 gaatttgaga aaaactataa agatgcatta agtgtaggtt ttaaaaatat taatgtagat
     5761 ttaatgtaca gtcttccaaa tcaaactcta tgtgactgga aggaaactct tgaaaaagtt
     5821 gtacacttaa atccaacaca tatatcagcg tattccctta tactagaaga aggaactgaa
     5881 ctatataata tgtatgaaag taataaattt gagcttatag atgaaaatgt agatatagaa
     5941 atgtatgagt atacaataaa ctacttaaag tcaaaaggat ataatcaata tgaaatatca
     6001 aattactcaa aagaaggcta taattgtgag cataatattt tatattggga atgtgaacat
     6061 tatataggga ttggagcagg agcttctggc tatattaatg agaacagata caataatgta
     6121 gaatctttag aagattatca tttaagtttg gtaaaaagag aaaaaccaat acaagaaaat
     6181 gaaattcttt ctgagaaaga tatgatagaa gaaaagatat ttatggggct tagaatgaat
     6241 aaaggcataa aatttgaaga ttttaaaaaa aaatttggaa tagattttag agagaagtat
     6301 aataagcaaa tagaaatgct attagctaga aagcttataa atcagagctt tgaaggtata
     6361 caattaactc aaaaaggaag agaaatatct aatagtgtat ttatagaatt tatggaataa
     6421 tttttaaggt gttgacaaaa taaatttata atggtatata atatatataa tctttttagc
     6481 actcgaactt agtgagtgct aacagctagg aggtataata atatggaatt aaatgaaaga
     6541 aaattaaata tcctcaaagc tatagttaaa gattatatag aaacagctga agctataggt
     6601 tctagaacaa tatctaaaag acatgattta ggtgttagtg ctgctaccat aagaaatgaa
     6661 atggctgacc ttgaagaatt agggtattta attcagcctc atacttctgc gggtagagtt
     6721 ccatctgaaa aaggctataa gttatatgta aactctctta tgagtaaaag cgaattggat
     6781 gataatgata agattcttat tgaacaatgt atgaatcata atataaatca tataaaagaa
     6841 ttgattcatg aaacctctaa gctattatcg caactaacaa actatacaac tgtagcagtt
     6901 actaagagtt taataaatca aagtgttata aagcacatac agttggttgc tatgaatgac
     6961 aataatattt tacttatagt tgtaacagat aaaggggact taaaaaaagc taaccttaca
     7021 actaatgtat atttagatca atctaagtta aatttgattt ctgataatct tacaaggaag
     7081 cttttaggta aaagtataac agatttggat gataatctaa ttgcttttat taaatacgaa
     7141 ataagtgagt attctggact tatagatgag cttttaaatg ctttgaactc aaatatgaaa
     7201 gaagaagatt tttctctatc attaaatgga gctactaaca tatttagtta tccagaattt
     7261 aatgatgtat taaaagcaaa gtcattttta aatatgttgg aaaaaaagga aacaatagct
     7321 gacataatta aatcaaaggg aatacaaaaa gacaatctaa atataataat tggtagtgat
     7381 aatgattgtg aactggcaca ggattgtagt atagttacag caacttataa tgtagataga
     7441 gacttggttg ggcgaataag ctttataggc cctaccagaa tggattatgc tagaatttac
     7501 tctataataa attatatgag tttattaatt aatagaaagt aaagaaattt taaattgcga
     7561 ggtgtaaagt cttggataaa aaaaatgagc aacaggaagt aagagaagaa aatgatactt
     7621 ctattaacca agaatcggaa actcaagtag agttagaaga ggaagttgtt aatgaagaat
     7681 gtgaaacatc aagtgaaaaa actgatgaaa aagaagttga tgatgaaaat gtaacagata
     7741 taaattctaa gttagcagaa aaaaaattgc aggatgagtt ggatgaatta aatgataaat
     7801 atcaaagact tcaagccgaa tatgcaaact acagaagaag aactcaacaa gagaaagaga
     7861 caataggcgt atttgcaaat gaaaaaataa taactgagtt gataccagtt atagatagta
     7921 tggaaagagc cttagatgca tgtgaagata aggaagatac tatgtataag ggtataagcc
     7981 ttgtacataa acaattaata gacactttgg taaaatttgg agtagaagaa atagaagcag
     8041 aatctaagga atttgaccct aatctacatc tagcagttat gcaagaaagt gtagatggag
     8101 ttgaagcaaa tcaaatagta atggtacttc aaaaaggata taagctagga actaaagttg
     8161 taagaccttc aatggttaaa gtttcttgct aagtataaat taaaggttaa attatttaac
     8221 ctaatataaa atattaaaat aagtaaaact taattatata attactcgag gaggatttta
     8281 gtcatgggaa aaataatagg aatagattta ggaacaacaa attcatgtgt tgcagtgtta
     8341 gaaggtggag aagcacaaat aatagcaaat agtgaaggta tgagaactac tccatcagta
     8401 gtagcattta caaaagatgg tgagagaata gttggagaac ctgcaaaaag acaagcagtt
     8461 acaaatgcag ataaaacaat aacttctata aaaactcata tgggaactga ttataaagta
     8521 aatatagatg gaaaatcata tactccacaa gaaatatcag caataatatt acaaaaatta
     8581 aaatcagatg cagaaagtta cttaggacaa acagtaacag aagcagttat aacagtacca
     8641 gcttacttta cagatgctca aagacaagcg actaaagatg ctggtagaat agcagggctt
     8701 gatgttaaga gaataataaa tgagccaaca gcagcagctc ttgcttatgg tatggataaa
     8761 ttagaccaag aaaagaaaat attagtattt gacttaggtg gaggaacttt tgacgtatct
     8821 atacttgaaa taggagatgg aacttttgaa gttttagcta cagctggtaa caatagatta
     8881 ggtggagatg actttgacca aatagtaata gattatttag cagatgaatt taaaaaagct
     8941 gaaggtgtag atttaagaaa tgataaaatg gctcttcaaa gattaaaaga agcagcagaa
     9001 aaggctaaga aagaattatc atcaacaatg agttcaaaca taaacttacc tttcataact
     9061 gctacagcag aaggaccaaa acacttaaat atagatttat caagagctaa atttgaagaa
     9121 ttaactagag gtttagttga aaaaactatg gaaccaacta agacagcttt acaagatgca
     9181 ggactttcta caggtgatat agatgatgta ttattagtag gtggttctac aagaatacca
     9241 gcagtacaag aagctgttaa gaagtttata ggaaaagaac ctcataaagg tataaaccca
     9301 gatgagtgtg ttgcagcagg tgcttctata caagctggag ttttagcagg ggatgttaaa
     9361 gatttattat tactagatgt tactccatta tctcttggta tagaaactat gggtaatgta
     9421 atgactaaga taatagaaag aaatactaca ataccaacta agaagtctca aatattctca
     9481 actgctgctg ataaccaaac agctgttgat atacatgttc ttcaaggtga aagaagtatg
     9541 gcttatgaca atacaacttt aggaagattc caattaacag atataccacc agcacaaaga
     9601 ggaatacctc aaatagaagt tacttttgat atagatgcaa atggtatagt taatgtttca
     9661 gctaaagatt taggtacagg aaaagagcaa aaaataacta taacatcaaa cactaattta
     9721 tcagaagcag aaatagagca aaagataaaa gaagcagaaa tgaatgcaga agctgacaaa
     9781 caaaagaaag aaaaaataga agcattcaat caagctgaat ctactatata tcaaactgaa
     9841 aaaactttaa atgaacttgg agataaaata agttcaggtg aaaaagagga tatagaaaaa
     9901 gctatagcag acttaaaagc tgtaaaagat aatcaagatg ctacagcaga agaacttaag
     9961 aaagctacag atgaagtaat gactaagttc caaaaagtat ctcaagaaat gtaccaaaaa
    10021 gcagctcaag aacaacaagc ggcacaaggt gcagaacaag cacaagacaa tggacctaaa
    10081 gatgacaatg tagtagatgc tgactttaaa gaagtagatg aagataaata agctatataa
    10141 tatgtaacac taaatgataa ttggaacata tatgatttgt gaaaatcata tagatgttaa
    10201 agatatatgg acaatacaca tcataaagaa tataatatat catagattat ataacttaaa
    10261 gtagaatata agaattaatg atagcttgta gtttattatt ctacaagcta tcgttttgta
    10321 aaatgaaggt ggtgtcaatt tttgagtaca aaaagggatt attatgaagt cttaggtatc
    10381 agtaaagatg cagaagctca agagataaaa aaagcttata gaaaattagc aatgaagtac
    10441 catccagata gaaatcctgg agataaagag gcggaagaaa aatttaaaga gataaatgaa
    10501 gcttatgaag ttctatctga tgatacaaag agaaaaactt atgaccagtt tggacatgat
    10561 ggattaaatg gtcaaggtgg ttttggtggt caaggaggat ttggcggtca aggctttggt
    10621 ggttttgaag atatgttcgg agatatattt ggtgacatgt ttggaggaag cttcggaggt
    10681 ggaagagcaa gaagaagagg gcctcaacgt ggtgcagata taagacagag tgttactata
    10741 tcttttgaag aagctgcatt tggtaaaaag atgtctataa aagtaaatag aagtgaagag
    10801 tgtgaagaat gtaatggaac tggagcaaaa ccaggaacat caaagaaaac ttgttcaact
    10861 tgtaatggaa ctggccaagt tagaactgtt caaagaactc catttggtaa tatagcaagt
    10921 tcaagaccat gtagtgcctg taatggaact ggagaagtga ttgaatctcc atgtagtaaa
    10981 tgtcatggaa ctggaaatac aagaaaagtt aagactatag aagtagatat acctgctggt
    11041 atagatgatg gtcaaatgat aaaactttct ggtcaaggtg aagtaggaga aaaaggtgct
    11101 ccaagaggag acttatatat tgtagttaat gttaagtcac atccactatt tactagagat
    11161 ggaaatgata tttattttga aatgccaatt acatttgttc aagcaacttt aggtgatgag
    11221 atagaagtac ctactttgga tggaaaagtt aaatatagtg tgccagaggg gacacaaact
    11281 ggaactgtat ttagacttaa agagaaagga atccctagaa ttagaggtaa ttcaagagga
    11341 gaccaatatg ttaaggttgt agttgaaatt cctaaaaaat taaacgataa gcaaaaagaa
    11401 ctattgagag aatttgcgaa ggagtgtggg tcgaacgtcc acgaaaagaa gaaaaccttc
    11461 ggacaaaaga tagaagatat gtttaagaaa aagtagttaa agtagccaat atataatcat
    11521 atttttgaaa tcttagttat ttataagatt gtcaaaggag atattatgat ttttatattg
    11581 gctatttttg tataaaaaat aaatgtgcat aaaataaaac tgaatataaa taattttctg
    11641 aaaaatactt gtcctaaaat atataatgtg ctataatata atcaaataaa agaaaggggt
    11701 gattccagtg ggaacggatg tggcactgcc aaatgatgaa gaaaacttta ttgtaatata
    11761 caaaataaaa ttagatactt ttagtgctag attaagtact ggaaaaactc atgagctggg
    11821 ataattattg taattaccta gactcaaact tttagtagta aaatagtaat attatataga
    11881 caaggtatct aatttttaaa tcttttgtat gagttaaaat ttttataaag ggtatatctg
    11941 attagaatgt aattagggaa acctagttgt gaattacttt tagtatatac atgtgtaaag
    12001 aaatatgtat atggtattgg ttattgtgct agatgtgtat gtaaaggaat atgtgtctag
    12061 ttgggtatat gtaaagaaat atacttataa tctatgaaaa atttttagta taaattgcta
    12121 aaacattgag attgtttttt atagaaagtt agtttatacc aactaaaaat gtgcttaaga
    12181 tttgtttgat gtttaattat aaataaggtt gtagataaaa aaataagtaa agacaaaggt
    12241 ttaaaatact tgctttaaat atagtttgtt ttgaacactt gccaaattaa aataattaat
    12301 aaaatagaca tcttctcata gtaaaatata ctgtgagagg atgtttttgc tttgttcata
    12361 aacttttatg aaaaatttgc atagtgtcac attttaatat actatatata taataaaata
    12421 tagataaaat taaatgattc aaaagagaaa ggtgaaaaga gatgtattat ataggtgtag
    12481 acataggagg aacaggtata caagcaggag tagttgataa ctatgggaag ataattttta
    12541 ggtcagagtg taaaacagta attgaaaaag gctttgaagg aatacttaat gacataaaaa
    12601 taatgattta taagttgttg gaagataata aacttactat gagtgacata aaatctattg
    12661 gatttggtgt tccaggtttt ataaataaag aaggattagt aacttgtgta aatttaaaat
    12721 ggaataaaaa agcttttaat aaagagttaa aaagaagatt tccagatgct gaaattcatg
    12781 gagaaaatga tgcaacagta gcagcacttg gagaagcaaa gtttggaagt atgaaaggtg
    12841 ctaatgttgg agtattatat actttaggaa ctggtgtagg tggaggaata gtaattaatc
    12901 aaaaagtctt ttctggagca catggattgg gttcagaaat aggtcatcaa attataggag
    12961 aaaactattt taattgtaat tgtggaaata atggttgtgt agaaactttt tgttcagcaa
    13021 ctgcaattat aaagtattct caaaaactga tagaagaagg agaaaagagt aggattttag
    13081 atttggcaga gggaaattta gaaaatgtaa atgcaaagat ggtgtttgat gcatatagag
    13141 aaaatgattt agtggcaatt aaagtaataa atagatttaa agattattta gcaaaaactt
    13201 ttgcaaacac aataaattct ttagacccag aaattatatc tataggagga ggcatttcta
    13261 aatctagcga tataatactt gatggaatag aagatttggt aagaaaattt gtattataca
    13321 aaacagaaga tatagctaca attacatgtg caactttagg ttcagacgca ggaattatag
    13381 gagcagcttt tttataatgt aaatgaactg cttattggag gcgtgatgat atatgggtaa
    13441 aacaaatgct agaagaacgc tttttttgat agcaataggt tcaggaacta tgctgaatcc
    13501 tcttaattct tctatgattt cacttgcttt acatagtata caaaatgatt ttcatattag
    13561 ttttacaact gtatcttgga ttgtttcagc attttaccta gctagtgcta ttgcacaacc
    13621 agtttcaggt aagataggag atttaatagg tagaaaggtc ttatttttga gtggtttgat
    13681 gcttgtaatt ttatcagcat ccatagtacc acttgttcaa tcattcttta tattgatttt
    13741 tataagagta attcaagcaa ttggaacaag taccctatat ccatctggag ttgctcttat
    13801 acaaaataat ataaaagaaa gacaatcttc tgcattagca gttctaacca tttttgcttc
    13861 aactacagca ggattaggtc cgacacttgg aggattattg ttggacttag gaggatggca
    13921 tgctatattt cttgtaaata tacctgttgt tttggtttct atatgcttag ggtatttttt
    13981 attcccaaaa gaagtaaaag aaaagaaatc tataaaagaa actttaaaaa atgttttgtc
    14041 aagattagat attattggca tacttctatt ttcaattgca atgattttta tattactatt
    14101 tttgctatct atgaaggaat cttttaactt agaacaactt gtatttggta taatactgat
    14161 gtgtcttttt atttggcatg aattgagaac aaaattgcca tttatagata ttaagctttt
    14221 tgtaagtaat cctaaattgt caaaagttta cttgcaattt attatattaa atttctttaa
    14281 ttatattttg ttttttggtc ttcctagtta ttttcaagat gccttacatt atagtgcaaa
    14341 gtcgactgga ttatttatgc tatttatgtc tggtataggt atatttatat ctccattaac
    14401 tggaaaatgg atagataaat ctggaactag atttcctctt gttacatcat caatctttat
    14461 gtttattagt gcagtacttc tttcagtagt ttttatccat atacctgtaa taggaaaggg
    14521 tattatatta tcattagcag gtattagtta tggagttggt aatgtagccc tgcaatcctc
    14581 tatgtttgaa gaaagtcctc gtgacagtat agggacagct tctggtctgt ttcaaactag
    14641 tcgttattta ggttctattt tagcaactgt tttacttgga atggtatttg aaggaaatat
    14701 aacatcagaa caatttcaga tattaggtta tgttatggtg gtcttaggta caataagttt
    14761 tttattaaat tttatattaa agaaagaatt aagaaaagta gaatagtgga tttatggatt
    14821 taataagtct aataactgat aagtttaaaa atattgtttg taaaacataa ctggtttgat
    14881 atttattaat tctaagtttt gaagcataga gtactcgata agtatacaat attttgaatt
    14941 aaaattaagc agaaatttga actagtcttt caaatctctg cttaatttag tagttaaatt
    15001 tttttataga gaaaataaga tttgacactt agataaaaaa tcattgcatt ttcaaaattc
    15061 tttaaatcaa acccaagtat ctcattaatc ttttccaatc tatatactag cgtgtttcta
    15121 tgtaaaaaca tactagtaga tgtcttagaa atatttaaat cattatttat aaattttatt
    15181 cctgtatata gaagttcttc gtcaatagat ttaatcttat taaaatctat taatttattg
    15241 agtatattat cctcattaat taaatctaaa gttttatatc ta
//
LOCUS       DAEPOZ010000086        15276 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.086, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000086 DAEPOZ010000000
VERSION     DAEPOZ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15276
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.086"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..268
                     /locus_tag="KR440_001915"
     CDS             <1..268
                     /locus_tag="KR440_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBH0698966.1"
                     /translation="SITSILEIVVYYFVNSMVTVSALIFLYIPQTQTASISILKLDEI
                     GYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            280..1347
                     /locus_tag="KR440_001916"
     CDS             280..1347
                     /locus_tag="KR440_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH0698967.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
     gene            1833..1949
                     /locus_tag="KR440_001917"
     CDS             1833..1949
                     /locus_tag="KR440_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein"
                     /protein_id="HBH0698968.1"
                     /translation="MDDDINMIAIRPEDIRVEFNRDFKEIIDLKLRPYRYKN"
     gene            2113..3180
                     /locus_tag="KR440_001918"
     CDS             2113..3180
                     /locus_tag="KR440_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH0698969.1"
                     /translation="MKFKKLVSLGILSTLLLSVITGCSNVSSGSNLKSNNKTKGELTV
                     YTAIEEDSIDTYLATFKEKYPDIKLNVLRLSVGGITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNPAFKDDKKVPSWVGIDAWMTGITVNTKELKAKNISA
                     PNSYEDLIKPEYKDLISMPSPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            3372..3902
                     /gene="rnmV"
                     /locus_tag="KR440_001919"
     CDS             3372..3902
                     /gene="rnmV"
                     /locus_tag="KR440_001919"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBH0698970.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMQRIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            4073..4942
                     /gene="rsmA"
                     /locus_tag="KR440_001920"
     CDS             4073..4942
                     /gene="rsmA"
                     /locus_tag="KR440_001920"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBH0698971.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREILKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            5008..6279
                     /locus_tag="KR440_001921"
     CDS             5008..6279
                     /locus_tag="KR440_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698972.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDSDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEEYEELEEDEEIEDNEESDRDEDS
                     EEEYIIEEYDEDEYEDYWEDDEDIEEGELKARTEVEDSGDEEIEEFNNYKEIENYNRV
                     KVQEEYIDIEYDEEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGV
                     SQGESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMP
                     YLGYLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGST
                     GFLRYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            6430..7545
                     /locus_tag="KR440_001922"
     CDS             6430..7545
                     /locus_tag="KR440_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBH0698973.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIVAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            7731..8627
                     /locus_tag="KR440_001923"
     CDS             7731..8627
                     /locus_tag="KR440_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBH0698974.1"
                     /translation="METRYEEANKITIQSILWNVVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASIVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            8697..9494
                     /locus_tag="KR440_001924"
     CDS             8697..9494
                     /locus_tag="KR440_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698975.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(9550..10902)
                     /locus_tag="KR440_001925"
     CDS             complement(9550..10902)
                     /locus_tag="KR440_001925"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBH0698976.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            11121..11999
                     /gene="purR"
                     /locus_tag="KR440_001926"
     CDS             11121..11999
                     /gene="purR"
                     /locus_tag="KR440_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBH0698977.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            12155..12433
                     /gene="spoVG"
                     /locus_tag="KR440_001927"
     CDS             12155..12433
                     /gene="spoVG"
                     /locus_tag="KR440_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HBH0698978.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            12643..14022
                     /gene="glmU"
                     /locus_tag="KR440_001928"
     CDS             12643..14022
                     /gene="glmU"
                     /locus_tag="KR440_001928"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBH0698979.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEAEVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSNVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SVVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            14117..15067
                     /locus_tag="KR440_001929"
     CDS             14117..15067
                     /locus_tag="KR440_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HBH0698980.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
BASE COUNT         6181 a         1646 c         2800 g         4649 t
ORIGIN      
        1 gtctataaca tcaatacttg aaatagtagt atattatttt gtaaattcaa tggttactgt
       61 ttcagcatta atatttttat atatacctca aactcagact gcatcaatat caatactaaa
      121 gttagatgag ataggatata taggaccatc agctgctatg gcagtattag tgctattaac
      181 aaatataata gtaagacttt tgtacgaatt tgtaacaaag aaattaaaaa ataacacaca
      241 aaaatggcag caaaaagatg ttgcataggg gaggaaaaaa tgaaatttaa aaagctagtt
      301 tctttaggag tattatcaac acttttatta agtgtaataa caggatgtag tactgattca
      361 tcaggttcag attcaaagtc tgatagtaag acaaaagggg atttaacagt ttacacagca
      421 attgaagaag atagtataga gccatattta gctacattta aagagaaata tccagatatt
      481 aaattaaata ttgttagggc atcaacagga gatataacag caagattatt agcagaaaag
      541 gataatcctc aagcagatgt tgtatgggga gttgcagcca caagcttatt ggttgcagat
      601 gaccagggga tgttagaacc atatgctcca aaaggttctg aagaaataga gtctgcattt
      661 aaagatgata aagaagttcc ttcttgggtt gggatagatg cttggatgac aggtataaca
      721 gtaaacacta aagaattaag tgataagaac atcccagttc ctagttctta cgaagattta
      781 ataaagccag aatataaagg tcttatatct atgcctaatc catcttcttc tggtacaggg
      841 tacttaacag tatcagccct tatacaaatt atgggggaag aaaaagcatg gcagtatatg
      901 gataagttgc atgagaatat tggtgtatac actcaatctg gttcagctcc agctacatca
      961 gcagcttcag gagaatatcc tattggtata tcttttggat atagaggtat aaaattaaaa
     1021 gaggaaggct atccagtaga agtagtattt ccaaaagaag gttctggatg ggatatagaa
     1081 gcaaatgctc ttgtgaaaaa agataatata aaagaagcat caaaagtatt tttagattgg
     1141 gctataagta aagatgcaat gaatgaatac tctaagaact atgcagttac tactatttct
     1201 acaggaaatc caataccaga aggattccct aaaaagccat tagagcaaat gatagataat
     1261 gatttaaaat cagcagcaaa aaatagagaa gatatactaa ataaatggat atcaaaatat
     1321 gatggtaaga cagaaaaaga gagttaagta aaactataca aatggagtaa aaagaaaaaa
     1381 tttttttaag tagattttat tataataata taagttgaga ataatatagg tcaagaaaag
     1441 tgaatattct ataaaaatgt atagagtaag tataccattc agtttgaatg gtatattttt
     1501 tatttttaga gattaattta gaaatagata tactacagtt taaaatagta tattttaatt
     1561 tcattattaa agtatagatg ttatatataa agtaaataag atattaaata attataaact
     1621 aaagtaaata aaatattgag cgattattaa aaagatatat atatatattt taatttgagc
     1681 agtttgttat atattataaa aaaagagaaa aataaaaggt aaattgtttc tggataactt
     1741 aaaattggaa gtaagaatat ttaactagag aagaattatt taagttcagg ttagctctag
     1801 gagtagctga ttttatagga gcaatagatt ttatggatga tgatataaac atgatagcaa
     1861 ttagaccaga agatataagg gtagaattta atagagattt caaagaaatt atagacctca
     1921 aactcagacc gtatcgatat aaaaactaaa gctagatgat atagtgttgt atataggacc
     1981 atcagctgtt atggaagtat tagtgctttt aacaaatata atagtaaaac ttttgtatga
     2041 attgataata aaaaaattaa aaaccaagac ataaaaatgg caacaaaaag aagttgaata
     2101 agggaggtaa aaatgaaatt taagaagtta gtctctttgg gaatattatc aacactttta
     2161 ttgagtgtaa taactggatg tagtaatgtt tcatcaggtt caaacttaaa gtctaataat
     2221 aagacaaaag gagagttaac agtttataca gcaattgagg aagatagtat agatacatac
     2281 ttagctacat ttaaagagaa atatccagat attaaattga atgtacttag attatcagta
     2341 ggaggtataa cagcaagatt attagcagaa aaagataatc ctaaagcaga tgtgttatgg
     2401 ggtgttgcag ctacaagttt gataatttta gatgacaaag aaatgctaga gccatacgct
     2461 ccgaaaggct ttgataaggt aaatcctgca tttaaagatg ataagaaagt tccttcttgg
     2521 gttggaatag atgcttggat gactggtata acagtaaata ctaaagaatt aaaagctaaa
     2581 aacatatcag ctcctaattc ttatgaagat ttaataaagc cagaatataa agatcttata
     2641 tctatgccta gtccagcttc ttcaggaaca ggatatttaa cagtatcaga actcatacaa
     2701 attatgggtg aagaaaaagc atggcagtat atggataaat tgcataaaaa tattggtgta
     2761 tacactcagt ctggttcagc gccagctata ttagcagctt caggagaata tcccattggt
     2821 atatcttttg gaaatagagg tataaagtta aaaggtgaag gttatccagt agaagtagta
     2881 tttccaaaag aaggttctgg atgggagata gaatcaaatg cacttgtgaa aaaagataat
     2941 ataaaagaag catcaaaagt atttttagat tgggctataa gtaaagatgc aatgaatgag
     3001 tattctaaga attttgcagt tactacaatt gacacgggaa atccggtgcc agaaggattt
     3061 ccaaagaatc cattagaaca gatgatagat aatgatttaa aatcagcagc aagaaataga
     3121 gaggctatac taaataaatg gatagaaaaa tatgatggaa agacagaaaa agagagctaa
     3181 gccttacaat aaaatttagt acaaaaagcg cctagaatat aagttttaca tctaggctct
     3241 tttgatatat ttatttaaaa tgcatatttc tatataaaaa taaatagttt agtaccttga
     3301 ttaaattaaa taaaaacctt aaaataaaga gatgtaaaga aaaactaaaa acgaataaaa
     3361 ggtgaattta tatgataaaa gaaataatag tagtagaggg tagagatgac gttacagcag
     3421 ttaagagagc aatagatgca gaacttataa ctacaggtgg atttggattt ccaaaaggtg
     3481 taatgcagcg tataaaagct gctaatgaaa gacgtggtgt tataatattt acagacccag
     3541 attttgcagg tgaaaaaata agaaaaaaaa tagcatcaga agttccagga tgtaaacatg
     3601 catttctacc aagagaggaa gctaaaaagg atggagatat aggtattgag aatgctactc
     3661 ctcaaagtat aatagcagct ctgaataaag ttagaactga aaatacagaa aaaagagatg
     3721 aatttaagca agttgactta attagaaatg gtcttatagg taatgaagaa gcatcactta
     3781 gaagagatgc acttggaaaa atacttggaa ttggatatgg aaatgcaaaa caatttttaa
     3841 atagattgaa taattatgga gttgaaagag aagaatttat agatgcatta aaacaaatct
     3901 aaacgtaatg tttaatgtat gaaaaatatg tatataaaat aaattgtgga attacggtta
     3961 atttaagata aattatttat aatatatacc ttattttaaa tcaatgtaaa tacatataaa
     4021 taagtataat taataaaatt aaatatcaaa ataagttaaa aaggagtaaa taatggatag
     4081 actatcttca cataatgcaa caaaggaagt agtacaaaaa tataatttta aattttcaaa
     4141 gtctttaggt caaaatttct tgatagatag taatataatt gataaaatct tatctggagc
     4201 tagaatcaca agaggagata atatcataga agtaggacct ggtataggaa ctcttactcg
     4261 tgagatgggt aaaatagcag aaaaagtagt ggctatagaa atcgatagaa atttaattcc
     4321 tatattgaaa gataccttgt cagatttaga taatacagag gttgtaaatc aagatatact
     4381 aaaagtagac atacaggaat tagtaaaaga taaattaaat ggtggaccag ttaagcttgt
     4441 agcaaatctt ccatattata taactactcc aatagttatg aaatttttag aggaagacat
     4501 acctgtaaca gacatagtag tcatggttca aaaagaagtt gcagatagaa tgaatgctat
     4561 tccgggtact aaagattatg gggcattatc tatagctgtt caatactatt gtgatacaga
     4621 gattgtggca aaagctccaa gacatatgtt tataccacaa ccaaatgtag attccacagt
     4681 tataggttta catgtaagag ataaaagaaa atatgatgtt cataatgaag acatattttt
     4741 taaaacagta aaagcatcct ttggacaaag aagaaaaact cttcttaatt cgttgggagg
     4801 tcttggtttt cttaataaag atgaaataag agaaatatta aaagaagcta atatagacga
     4861 aaaaagaagg ggtgaaactc ttagtataga agagttttca gttctttcaa atataataaa
     4921 tacaaaagta tcttctaaat agaaggtact ttttttaatt ttgttaacat atattgttta
     4981 gagaaactgt gttaaggggg gattgcagtg ggttctaaaa gaaagtttaa aagagactat
     5041 ataatactag aagctaaaga cataaacttt agatacaaag aacgagtact gccaaaagct
     5101 tttgcaaaag ttgaagtaaa tgatgaaaaa gcaattattg cattatatgt agaaaatcta
     5161 aaatatgtta aagatgggta taaagcagta gcaataaaag atgaccatgg attaatagac
     5221 ttaggaaata taattttaaa tgaacaagga agaggtgaat ttgttttaga tttagaagat
     5281 tctgatgatg agataaaagg tattgcatta ttgtatgaaa gaaatatacc tttaattgga
     5341 tttaagggaa ataagcttga aaattatgag gacatattat ttcaagaggt agaggaagaa
     5401 gaatatgaag aatatgaaga atatgaagag ttggaagaag acgaagagat agaggataat
     5461 gaggaaagtg atagagacga agatagtgaa gaagaatata ttatagaaga atatgatgaa
     5521 gatgagtatg aagactattg ggaagatgat gaagatattg aagaaggaga gttaaaagca
     5581 agaacggaag tagaagattc tggtgatgag gaaatagaag agttcaataa ttataaggaa
     5641 atagaaaatt ataatagggt taaagtacag gaagaatata tagacataga atatgatgag
     5701 gaagaagagg aagaagaaga agtttttgaa gtaaatagag aagaagatag gtttgtttcc
     5761 tcaaaaccta ctaaatcaaa gaaagttcaa aaagatcaga ataatattga aaaaataaaa
     5821 agagctaatg gagtaagtca aggagagagt ggaaataaac aaaccagtac attacttatg
     5881 ccaagacaaa taaaaaaagg tttgaaatta tttaaagaag taaaaccttt tgtaacagat
     5941 tatatagata agacaagatg gtggaaaata gagataaatc ctacaacact gtgtggatat
     6001 acaatgcctt atttaggata tttaaacgta ttgaattaca ctatgtatag tgatgcagtt
     6061 ttacattcat ataaatacag acactattta tttggtgttc agtatgatga atataataag
     6121 agaaaacatt atatttatgc tatcccagga aatagaaatg aacagccaga caatggaagc
     6181 acaggatttt taagatatca agcttgtgat aatagaacaa atagtttagg atattggcta
     6241 tgttttatag attctagaca aagaaatata gttaggtaaa aataataaaa atgaaaaaat
     6301 ttagactcaa ttcttatttt actgaaaaaa ttaatgaaat gtagtacaaa actttaaaaa
     6361 ctattgataa tatttaaata ttacactaca atatatcttg aaatacctat tatattatgg
     6421 aggaaaatta tgataaacga gcaaagaatt ttaaatgagt ttcttgaatt agtacagata
     6481 gatagtttat ctttaaaaga ggggaatgtt gctaaagtat tagttaaaaa attagaagaa
     6541 ataggttgtt cagtagtaat agacaatgct ggtgaaaaag caaatggaga aactggtaat
     6601 ataatagcta cattaaaagg aaataaagaa ggaaagaaaa tattatttag ttcacatatg
     6661 gatacagtaa ctccaggtat aggggtaaaa cctatagtgg atgaagcaaa tggaataata
     6721 aaaagtgatg gtactacaat attaggttct gatgataaag caggtatagc agctatatta
     6781 gaaggcttaa gatatataaa tgaaaataac atagaacata ctgatataca agttgtattc
     6841 tctatatgtg aggaatgtgg attagttgga gctaaaaatt tagactatgg aaaaatagat
     6901 tctgaatatg catttatatt agatagtggt ggttctccag gtgaaataat agtaaaagct
     6961 cctgcacaag atgtaataaa tgttaagata ttaggaaaga ctgctcatgc aggtcttgaa
     7021 cctgaagctg gaataagtgc tataatggta gctgccagag caatagaaaa tatgaattta
     7081 cttagaatag acgaagaaac aactgctaac ataggaatta taaacggtgg aacagctaca
     7141 aatatagtaa caggagaagt taatatagtt gcagaagcca gaagtctaaa agaaaataaa
     7201 ttagatgttc aaactaaaca catggtagag acttttgaaa aagctgcaaa agattttgga
     7261 gctcaaattg agatagatgt caatagagca tatacaccaa tagatgttag tgaggattct
     7321 gaaataataa aacttgcaaa gaaggctttt tcaaacttag gtatagaagg tcatactgaa
     7381 tctacaggag gaggtagcga tacaaatata ttaagtaaga atggtatcga agctataact
     7441 ttaggaattg gtatgaaaaa tgctcataca ctaagtgaac atatagctat aaaagactta
     7501 tatgactctg ctaaaatggt agtggagata ataaaggaag cataaattta aataatagtg
     7561 tataatatat atatcgccta gcttaagcta ggtgattttt cattttatag aagattgata
     7621 atctctattg aaagcaaaga gtatacattt gcatataaga gagtgtttaa tttatgttca
     7681 gatgtaggtt cttaataata tttagattgt ttcataaggg aggaataaat atggaaacaa
     7741 ggtatgaaga agcaaataaa ataacaatac aatcaatact ttggaatgta gtattaacaa
     7801 taattaaggt tatagctggt gttattggta attctagcgc tatgattgca gatggattac
     7861 attcagcatc agatattata agttctatag gagtactaat aggcaattat gtatcttcta
     7921 gacctggaga tagagagcac aattatggtc atgagaaagc agagacatta gtatcatttg
     7981 tgttatcaat attgcttatt ttcgtttcta taacaatagg tatagaggca attaaatctt
     8041 tatttaactt agatgcatta agtgttccat caatattacc attagtagta tcagttattt
     8101 caatcctgat aaaagagtat caatatagaa taacaataaa agttgctaaa aagataaatt
     8161 caccagcctt aaaagctgat gcttggcatc ataggtcaga tgctttatct tcagtagctg
     8221 cttttatagg tataggtggt tctattttag ggtttaagcc actagaccca attgcgtcaa
     8281 ttgtagtggc tatatttgta gctaaagtag gaattagtat acttataagt tctgttaatg
     8341 aattgatgga tgtttcagtt gacgaagaag agataaaaga attaaaattt attgtagcag
     8401 atacagaagg tgttaaaaat cttggtgata taaagactag aaaacatgga gctatggcat
     8461 atgtagattt gactatatgt gtggatgaaa atttgacagt taagcaaggt catgatattg
     8521 ctaccaagct tgaaaaacat ataataaaac atatggaatt tgtaaaaggc ataacagttc
     8581 acgttgaacc atgtactaat tgccaaggaa ataagtgtaa taattaatta aaacttaaaa
     8641 aaaagtgtaa aaagttatat aattgtttta tgattataaa ctctaggggt ggttaaatga
     8701 gcaagaaaag aaaactaaag aaaaaagctg caatattaat aggagtagtt gttttcttta
     8761 taatatactt tatatccttt agaataggaa taaatatgga aaaggataaa agtgaagtgc
     8821 caaagaaggt agaaaaagaa gaagaagata ataggtcttt aaggactcaa ataaaagaaa
     8881 agaaaaaggt ttatatatca gacaaaaata tagaaaacat aaaaattgaa gaagatatgt
     8941 gggatgaaat aaagttattt ttctcagatt ttaagaaagt aagaaatact acttcttctt
     9001 acgaagcagt ttatgaaggt tattctgatg atggagttaa attttctact gaccttaact
     9061 tttttagagt atatactgta aataaagaag aatattataa agtaccagtt agttctaagg
     9121 atgcttttaa gaaactttta gatgagagta tatatacttc atttgacctt ttaagtcatt
     9181 ataaagattg gaaagaggtt actataactc atggaaaaga aattaaaaaa gttcatagat
     9241 ggaaatttga tgatttagca cataagatga tttcaaagag ggttgtagga aaagttcaac
     9301 ctgagaaaag taaggaaaga agtgactata attttactat aaatataaaa ggtgataatt
     9361 ttgatgtaaa agttgataca atgggaaaag attatgtaaa agttacttct aagaaaaatg
     9421 tatcttacta tgaggttcat acaagtttat ttgattacct taaaaatgag atatttgaaa
     9481 ttggaaagaa ataagacttt aagaaggcta tcaaattaga tatttgatag cctaaattat
     9541 ttttatatat tatttttcaa ccaacaagtc tgcaacctta taaattggac cagcaccagc
     9601 agttattact aaatcattat ccttaacatt ttcacgtaag tattttacta tatcttcaaa
     9661 ttcttttata tagatggcat ctacattgtt ttgatataat ttctcaacca agtccttaga
     9721 atgtatatct ccagggtctt tttccctagc agcatatata tctgttatga taactttatc
     9781 agcagcatag aatgcctctg aaaattcacc taaaagagat tttgttctag tataagtatg
     9841 tggctggaag atacaccata aagttgattt ttttaatttt ttggcagcag ctaaagttgc
     9901 ttttaactca gttggatgat gtgcataatc atctactact aaagcattct tataataccc
     9961 tttagtttca aatcttcttc cgacaccttt ataaattttt atattttttc ttatagtctc
    10021 taagtcaata ccagaaacaa gagctgccat tatagcagaa gaagcattgt atatgttatg
    10081 aagaccatat acagaaagtt caaattctcc taaatctctt ccttcatatt ctattctaaa
    10141 tattccatga ccactattgt caaagtgaat atcttttatt acagcatcat tgtcagaatc
    10201 tctaccgtat tttataatag tagctttaac atcatacaat atgtcatcaa tattttcatc
    10261 atcgccattt attatgaaat agccttctgg gggtaataat tttccaaatt tgttaaaaga
    10321 agccttgatt tcatctattc cagagaaata atcaagatgg tcttcttcaa catttaaaac
    10381 tatagatatc tttgggttaa aattaaggaa actatctaca tattcacatg cttcagttat
    10441 aaagtggttg gaattaccta tcttaacatt accacctatc atacttaagt taccaccaac
    10501 tagtatagtt gggtctaagt ctgcatattc aaagattgta gataacatag atgttgtaga
    10561 agtttttcca tgtgttccag aaactgctat agaattttta tattccctca tgatttggcc
    10621 taaaaaagtc gctctgttca ttaccaattt atttttttct ttagcagcca caagttcttc
    10681 attgtctgga tgaactgctg ctgtataaac aaccatattt acatcatctg atatattatc
    10741 ttttttctga ccaatataaa tagttgcccc ttggtcccta agtttgtcta ataggtatga
    10801 ttcgtttgaa tccgaacctg aaacttgata acctttattg atacatattt cagctaatgc
    10861 actcatgctg ataccgccaa ttcctataaa gtgtatattc ataataaaac cacctttctt
    10921 tttaattaag tcctacaata tgaactattt atgtggtata ataatataat gttcaatatt
    10981 ttataattaa tatgcatata atattatatg tattttattt ttgaatataa taaaacataa
    11041 gtaatattat accacaataa gaattgttat gcattagtta gtttaacgtt atataaaggt
    11101 acttgtagga ggaaataata atgaaattta aaagaacaga gagaattgga gcaatagtta
    11161 aaatactgtc tgataatcca aataaaatat atacattaag ttattttaca aatcaattta
    11221 atgcagcaaa atcaacaata agtgaagatt tattagttgt aaaaaatgta tttgagaagc
    11281 tacatttagg taaagttata actatctcag gagcagcagg aggagtaaaa tatataccta
    11341 aaacttctat agcagaaaat gcagagttct taatggaatt atgtgagaga atatgtgaca
    11401 agtctagaat actatctggt ggttttttat acttgattga cctaatttat gaccctacaa
    11461 tagctgctaa gataggaaag atattcgcat caaatattga gtatgtagat gcagattatg
    11521 ttgtgacaat ggaaacaaaa ggtataccta tggcacttat gactgcaaaa gctatgaatt
    11581 tacctctagt tataataaga aaagatataa aggtatcaga aggtcctaca cttagcatga
    11641 catatgtgag tggaaacagc tctaaagttg aaagtatgag tttgccaaga aaagctctta
    11701 aaccagatag taaagttata attatagatg atttcatgag gggtggaggt actataaaag
    11761 gaatggtaga ccttatgaat gaatttggtg cagaggttat aggtactgga gtattcatat
    11821 ctacaacgaa tccttcagaa aaaatggtaa aagattatat ttcattgatt caattggatg
    11881 ttaatggaga taaaatagta gtagaaccaa acttaaagac ttttaaagat gaatatagaa
    11941 atgaagattt agacgaagaa gaagattcag aatttgaatt tgagatagat gaagattaat
    12001 aaaaaaaata tttagaaaaa tatgtaaaaa taaaaagagg atatccctag ttgttcatag
    12061 aattatttaa aaaacagttt atattaattt attccagcga taataatttt aatataaatt
    12121 aatttctgtc aaaatcaaag ggggatattt tggtatgaag ataactgacg taagagtaag
    12181 aaaattaaca gaagaaggta agatgaaatg tatagtttca ataactttcg ataatttgtt
    12241 tgtagtgcat gatataaaag ttatagaagg ccataatggg ctatttatag caatgcctag
    12301 cagaaaagta ggcgaaggaa atttcagaga tatagctcat ccaataaatg cagaaatgag
    12361 acaagtttta gaagatgcag ttttacaagc atatcatgaa gcattagttc aatgggaagt
    12421 agctgctgaa taataaatat ataaaatgga gagagcccga acatttaggc tctttttaaa
    12481 tgtagaattt ctattaaaat atgtactttt tagtaaaaaa gtgctatatt attagtgata
    12541 atgtatatgt tgtatacaaa ttgttagata aatgtattat aatgtatatt gattaagaga
    12601 ataatacgaa taaaattaaa ttagattgaa tggagtgtat caatgaactt taaagctata
    12661 atccttgctg ctggcaaagg aacaagaatg aagtctaaat acccaaaagt tatacataaa
    12721 gtatgtggaa aagaaatggt taaccatatt atagatgttt caaaaaaatc aggggtaaaa
    12781 gatactgtcg tgattttagg acatgaagct gaggtagtta aagaaaagct tgcagaagaa
    12841 attataatag ccatgcaaac agaacaactt gggacaggac atgctgtaaa gatggctaaa
    12901 gaatatatta atgatgaaga tacaatagtt gtactttgtg gagatactcc acttataaaa
    12961 gaagagacac tgaagagatt atttgaatat catatagaaa ataaatatca tgctacagtt
    13021 cttacaacaa gagttggaaa tccaacaggt tatggtagaa taataagaga taagaagggt
    13081 gaccttctta aaatagtgga acaaaaagat gcaaattcag aagaaaagat gataagtgag
    13141 ataaactctg gaatttattg ttttaatgga aagagtctta gagaagcact agatttgcta
    13201 aataataaca attcacaagg tgaatattat ttaactgata ctgctaaaat aatgagagat
    13261 aaaggattaa aagttggtgc atttgcaggt tcaactattg aagaacttat gggcgttaat
    13321 tcaagagtag aattatcaaa ggcagaagaa attatgagac gaagaataaa tgaatctcat
    13381 atggtaaatg gagtaactat aatagacact aactctacct atatagaatc tgatgttatg
    13441 ataggcaatg atactataat atatccaggt gttatgttac aagggaaaac aaggataggt
    13501 tcagattgta taattggaat gaactcatct ataacaaatt cagaaatagg tgatggtaca
    13561 gaaataaaaa attcaactat aattgatagt aaggttggag aaaattctaa tgttgggcca
    13621 tatgcatact taagaccaaa gagtgactta ggaaataatg taaaaatagg cgattttgta
    13681 gaagtaaaaa atgctattat agaagatgga tcaaaagcat ctcacctttc ttatatagga
    13741 gatgcacatg tcggtaaaaa tgtaaatatt ggatgtggag tagtatttgt aaactatgat
    13801 ggtaaaaata aatttaaatc agtagtaaaa gacaatgcgt ttataggttc aaattctaat
    13861 ttagttgcac cagtagttgt agaagaaaaa ggatatatag caacaggttc aacaataaca
    13921 catgatgtcc cagatggtgc tttggcaata gcaagagaaa gacaagttat caaagaagga
    13981 tgggtagaaa aaaagaatca aaaagacgac caaagtaagt aattatgtag tttattattt
    14041 tggctaaaaa atttagctcg aaattattat aaattatatt cattagtatt ttaaaatttt
    14101 caggagggtt acataaatga atactagcgg aagcgagatt aaaattattg caggtaattc
    14161 atcgaaagag ttagcacaaa aaatagctga ctatataggt gtatcagtac tagattgtga
    14221 agtaggtaca tttagtgatg gtgaaatatg tgtaaacatg aatgagacag taagaggttg
    14281 tgatgtgttt gtagtgcaat ctactaacag cccagtaaat gataacttaa tggagttatt
    14341 aatcttaatt gatgcgttaa agagagcatc agcaggtaga ataacagcag tcattcctta
    14401 ctatggatat gcaagacaag acagaaaggc taaggcaaga gatccaatca ctgctaaatt
    14461 agttgcaaat ttaataactg ctgctggagc agacagagta ttaacaatgg atttacatgc
    14521 agctcaaata caaggatatt ttgatatacc attagaccac ttactaggtg gaacaatatt
    14581 agcaaattat tttaatgaaa agaaaataga agatttggta gtagtatctc ctgacttagg
    14641 aagtgttact agatcaagaa aatttgctaa tactttaaat ggagaagttc caatagctat
    14701 aatagataaa agaagaccga aagctaatgt atgcgaagtt atgaacttaa taggtgatgt
    14761 taaaggtaaa aatgttatac tattagacga tatgatagat acagcaggaa caatagttaa
    14821 tgctgcaaat gcacttaaag agtttggagc aaaagatgtt tatgcttgtt gtactcatgg
    14881 tgtgttatca ggtccagcta ttgaaagaat tgctaattca gaaataagtg agttaatagt
    14941 tcttgatact atacaacttc ctgaagagaa aagaatagat aagataaaga taaaaacagt
    15001 agctccttta tttggagatg ctataagaat gatattctct aatgaatcag taagtaaatt
    15061 attctaattt attgtattta tgagagttgt ttaaaaggat ataaaccttt tgagcaactc
    15121 ttttttgatt ttatacttat ggaatgttat ttttttcttg ttagaaaggt atatattatt
    15181 gtaaaaagag attatagttt aaaattatgt atatttaagt aaaataaact taataaaggg
    15241 catattattc aaaatattat gaatataaaa aatatt
//
LOCUS       DAEPOZ010000087        15204 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.087, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000087 DAEPOZ010000000
VERSION     DAEPOZ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15204
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.087"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(158..985)
                     /locus_tag="KR440_001930"
     CDS             complement(158..985)
                     /locus_tag="KR440_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBH0698981.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     gene            complement(1033..2469)
                     /locus_tag="KR440_001931"
     CDS             complement(1033..2469)
                     /locus_tag="KR440_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HBH0698982.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            complement(2556..3425)
                     /locus_tag="KR440_001932"
     CDS             complement(2556..3425)
                     /locus_tag="KR440_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBH0698983.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGNYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            complement(3583..4236)
                     /gene="phoU"
                     /locus_tag="KR440_001933"
     CDS             complement(3583..4236)
                     /gene="phoU"
                     /locus_tag="KR440_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBH0698984.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            complement(4264..5028)
                     /gene="pstB"
                     /locus_tag="KR440_001934"
     CDS             complement(4264..5028)
                     /gene="pstB"
                     /locus_tag="KR440_001934"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBH0698985.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            complement(5047..5871)
                     /gene="pstA"
                     /locus_tag="KR440_001935"
     CDS             complement(5047..5871)
                     /gene="pstA"
                     /locus_tag="KR440_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBH0698986.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVIIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            complement(5873..6790)
                     /gene="pstC"
                     /locus_tag="KR440_001936"
     CDS             complement(5873..6790)
                     /gene="pstC"
                     /locus_tag="KR440_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBH0698987.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            complement(7172..8407)
                     /locus_tag="KR440_001937"
     CDS             complement(7172..8407)
                     /locus_tag="KR440_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0698988.1"
                     /translation="MRIFYLEIKRVLHSRRTLILLLLALVLSALMAFLPITYEGINYL
                     DNNGKVVELSGLDAIKYKKTIYANNSEISSGVVTPKKLKHALNIWKKTEEKYGKVGSE
                     DFPLDIYSKSILPIRPLLNRLPEAFANSETGFATELSEINTNDLDSFYEQCKTHLNDI
                     MKIERQTSSAQMKAQELYRKVDTPFEIYPGYSKDAFDYIIFYIFILLIICVAITAPTF
                     SGEYQTQSDSILRCTKHGHTRLAFTKILALFSIFIVMFVVCISIHLAISDLAFGTECL
                     KTSFQMLFSVISLVNINLLQAQILIAIGGLISILAMVSLTLFLSAKCKDSLTSMLIAF
                     SICLIPMGIGVSWIGYLLPPGGIGLKISLLYQMTSFNFLHLGGMSFWTPHIILFFSVI
                     EIPIFIFLAIRTYCKHQVS"
     gene            complement(8408..9295)
                     /locus_tag="KR440_001938"
     CDS             complement(8408..9295)
                     /locus_tag="KR440_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0698989.1"
                     /translation="MELKIEHVTKKFKDKIAVEDVCLTLTPGVWGFLGANGAGKTTLM
                     RIITDIMKPTNGKILYNGKNIYIMGAEYRNLLGFLPQEFGFPQDFSVKDYLEYIAALK
                     DVPPREVKGKINNLLEILTLSDVKNKKIIKLSGGMKRRVGIAQAMLNDPKILIMDEPT
                     AGLDPGERVRFRNFISEFSHNRVVLISTHIVSDIEYISTQNAIMKSGKILDVGTTDKL
                     VKKVEGKVWECLISNRDISKYEMNLRIINQRSEKNNQVSIRYLAEKSQIDNSIAVEPR
                     LEDLYLWLFPEAVYSKEGK"
     gene            complement(9440..10555)
                     /locus_tag="KR440_001939"
     CDS             complement(9440..10555)
                     /locus_tag="KR440_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0698990.1"
                     /translation="MKKINNFTYRKLRDKYKFIKREKNTEAYLKYKLYIMLAIYTGIV
                     LVLMFMADYTFSNFSNPLVKWLKLRRDVLFFLVFIIGYITIFNFYWKKPWNYLREIIN
                     ATHTIYQQNDELVELSKPLKEIEQQMNQIKMSVLLSQQAVKEAENKKNNLVMYLAHDI
                     RTPLTSVIGYLSLLKEIPNMDNSQKSKYTEVALDKAQYLEKLINELFEITRYNSNQIK
                     INFEKVDLYYMLLQLKDEFYPLLSEKGNRAILKANESLTIKADSEKLARVFNNILKNA
                     IAYSYPNTEIIISAVHKDSKVDIAFRNYGQTIPEEQLSAIFEKFNRLDEARKSDMGGA
                     GLGLSIAKEIINLHGGDITVQSKDEVTTFTISLPTSI"
     gene            complement(10591..11289)
                     /gene="vanR"
                     /locus_tag="KR440_001940"
     CDS             complement(10591..11289)
                     /gene="vanR"
                     /locus_tag="KR440_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HBH0698991.1"
                     /translation="MSGKILVVDDERDIADLIEVYLTNENYIVYKFYSGLDALNCINL
                     NEFDLAILDVMLPGVNGFSLCQKIREKYTYPIIMLTAKDEETDKITGLTLGADDYVTK
                     PFRPLELVARVKAQIRRYKKYSGSLQSGEELSHIFSFLGLELNTQTYECHLNGKALSL
                     TPTEFSILHVLLKNKGSVVNSENLFHAIWKDEYYNKSNNTITVHIRHLREKLGDTVDE
                     AKYIKTIWGVGYKI"
     gene            complement(11671..12351)
                     /locus_tag="KR440_001941"
     CDS             complement(11671..12351)
                     /locus_tag="KR440_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0698992.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKEISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILTDGDGWTLCKKIRNVTTCPIVYMTYINEEQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            complement(12401..14416)
                     /locus_tag="KR440_001942"
     CDS             complement(12401..14416)
                     /locus_tag="KR440_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBH0698993.1"
                     /translation="MKKYNILKGTLIVLIVSLLAIAGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGN
                     QSPYGNATYRMRIKNEGEDKLLSLELPEIFSASEVWINGEIVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKDTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKLYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDSSY
                     FMVVLCIICINGSISKIEEDKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFIIVILFSLMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KKFLADVAHDLKAPVSAIQSFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNDFIKEIYENNLPDTQACGVNFETYILEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKVSDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            complement(14488..15165)
                     /locus_tag="KR440_001943"
     CDS             complement(14488..15165)
                     /locus_tag="KR440_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0698994.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVERI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
BASE COUNT         4930 a         2695 c         1647 g         5932 t
ORIGIN      
        1 atgtcaataa ttgcaagatt attttatttt aattttaatg ctttctcatt aattttatat
       61 tatattatcc ttacagtata cctcttattc gtatttttta tattatatgt tttattttat
      121 aaataattta ctttatcttt tactatattt aaatttatta taagttttct tgccagaaat
      181 ttttaggtgt tatattttct gtaattggca atattgtata tcctctctct tttaagattc
      241 ttataatatt atttaaagca tttaaacttt gctctttctc atgaattaat actactaatt
      301 catctctttt tcttccataa tataatatat tacttactat ctgacttgta gaagacttcc
      361 aatcctgtgt atccaaattc cagtcccaaa ttaaataatc atttgccata agtttattat
      421 atgaaccttc tggcatatat ggtttacttc cgtatggtaa tcttattaat tttgaactct
      481 gaccagttat ttcttgtaga gtattattgc atgtattaaa ttccccaact gttacttctg
      541 ggcttttata taacttatgt atatcatgac ttacactatg aaaacctatt gagttttcct
      601 cttccaagac tctccttact tcttccttgt gatttttcat attttgatta agcataaaaa
      661 atgtcgcttt gacatcattt tcttttaaaa tatcaagtag tgcatttgta tattttgatg
      721 gaccatcatc tattgttatg tatgctactt tttcagaagt agcccctgga ccttttgttt
      781 cttcttgaga tgctttgtat atgatttctc ctaaagtatt actttgagaa agcagttcca
      841 gtgtattttc accttttgaa cttatatttt caactggaac attattaatt ttttcctcca
      901 ataatgttcc agttaaaatt aaaacagcta taagaaatat ccttataaat acatgaaaaa
      961 cagccttatt atctttaatt ttcatccccc atatctccct tttataaaag cataagttta
     1021 agacttaaaa agttattttt tatatttatt ctctttctta ttacttttta tatctctact
     1081 aaatattttt ttaacctttt cttcttcttt ttctcctaaa gattctactc ttttgattcc
     1141 caaagaagtt aaaaattgta atacatttaa attatcaaat ggagtttcca tattcgtact
     1201 ttcactatat tcatcaacat tcttagtacc ttcttccaca cttaaaatac gcttcggacc
     1261 tcctacacag cctcctacac accccattcc ttctataaat gtagcttcta tttctttatt
     1321 tagaactttc tttagaccgt ccatgcactc ttttgttcct tcaaatgcct tttcttcaaa
     1381 ttttatattt ttgtcaattc ttctgactga taacttaact gctttactta cacctcctgt
     1441 tctagcataa actctacctg aaagggacgc ctctactcta ttactctcat ccattattgc
     1501 tggatttaac tctaatgcat caaatatttc ttgcaactct ttaaatgtaa gcacaaaatc
     1561 aactgcatct tttatatctt ctaacattgc ttctttcttt tttgccatac aaggacctat
     1621 gaatacaact ttagcatctg gatttaaaat ttttacagct ctcccacaag caatcatagg
     1681 agaaacggat ggtgatacat tctccaatat ctgaggaaaa ctattttgta tcaaactaac
     1741 ccaaatagga cagcaacaac tagttataaa gcatccttct tcgtcctttt tcatatgctc
     1801 ataataatca tatgcttctt ttgcagtaag catatctgct gccaaagcaa cttctatcat
     1861 atcttcaaat cctatctttt ttaatgcagt tcttaattta ccagggctta catctttccc
     1921 aaactgtcct acaaaagctg gtgcaactat tgcatataca gggctcttta tatcttttaa
     1981 caattttatc attggtataa attctatttt atctgaaatt gcacccaact tacatcttga
     2041 tacacaatca ccacaagcac tacatacatc ttcctcatta caattaatat gcttacttct
     2101 tatcttacat gactcatctt ccttattatt ttcatcacaa tatttgcaag catctgatat
     2161 tacagatatt attggtcttt tgacttcatc taaatttaca gcttcatgta gtgactcttt
     2221 taaattttta tctgtatctt ctgtcacatc caatcccata gttaccctaa ttaaatttac
     2281 catagtatct ttatcaaatt ttccactaaa ataattattt aaattatcct ttaaatcttc
     2341 atttaaaatt ttattaggta agttttcaat atcctctata tcatcattcc acactgcttt
     2401 tacaagttcg ccaaaaagct tcattttata tatttctagt attttagaat tgtcatttct
     2461 agtattcata aaaaatagta cctccataaa ttaaacttat atatgtttaa tatttacaaa
     2521 gatactattt tttatttatt atttaactac tattattaat atgatttatt tagctttctc
     2581 aacaactcta tttcaatcat tattttcatt agatgatagt tgaatacggg gcttttttct
     2641 tcatctatat tccagtctag cttatccaca ttatatgttt tcttaaggta tttatatgta
     2701 tgctctttca aataaagttt attttcaagt aactctatag actgcatatg aaaatgtatt
     2761 tctctacata tccttaaggc attctcaatt ttttctatat tagtttcact caaactataa
     2821 tccaactcat caataagttt tgaatatatt tcttctaatt tttctacttc ttcttcaaat
     2881 ttctctacat ttaaattttc ttttctaaga attttatctt caataagtaa cattgaaagt
     2941 tcttctatct ttttaaattc atctataaca ctatttagat aatttggtct agccacataa
     3001 taattaacaa gtactccaaa caaaacacct acagatgtat ctaacactct atgtaaagaa
     3061 taatacacag ggtcactatt tcctacacct agatgtatag ccagaaaggt tacacaggca
     3121 actactactg actttatttt aaacagattg cataaatata ttgagcacat tactcctaga
     3181 ccacataaaa aagggtctcc agctttaatg atggcaaata tgaatcctat tataccacct
     3241 aaaatagtac cttccactct attgattcct gtctttaaag aaccttttac cgtatcttga
     3301 acagaaatta tacatgctac tagagaataa aacatattct ctactagata gttaccaaga
     3361 atcatacata aagtgacact aattcctgtt tttatagtcc tcatacccat cttttgaagt
     3421 ttcaaagttc cacctcctaa aaagcacctt atataaaata cgcatcctca tatttaatat
     3481 gttcctagta tatcacaatt ttttaaattt acccattaat catatttttt tatcaattct
     3541 ttcttaattt agacaataaa aaaactgctt taaagcagtt ttttatccta tctcaaccat
     3601 tttaccctca attgcaaaaa ttatcttctc acatacattt gttatatggt ctccaattct
     3661 ttcaaggtat cttcctataa gtagaagttt tactccttgg tctatagtat ttgaattttc
     3721 atgcattatt tttaaacaat caatttgtac ttgttcatac aatccatcta caatatcatc
     3781 tttagttcca acatcataag ctatacttgc atcaaataag attaaagctt ctctagtctg
     3841 agacatcata gacttacatt cttttgccat cttaggaata tctatcagct cttttatata
     3901 agcctctcca cctatcttca acatttctct agctatattt gctgcataat ctcctattct
     3961 ttccaactct atcgatatat tacctaatga ataaataaat cttaagtccc ttgccattgg
     4021 ttgctttaat actattagct ctatactctt atctcttatt tcttctctta atttatttat
     4081 ttcttcatca tcttctacta cttttcttgc accctctaaa tccttctcta tcatacaagt
     4141 cacagacttt tctattgctt cttcacattt ttccatcata agtactgttt gttcttttaa
     4201 agtatcaata cttatatcta aactattatt gaccattgtc taactccttt atagttatat
     4261 attttaacca aatctacctg ttatatagtc ttctgttctt ttatctacag gagtggtaaa
     4321 catagttttt gtatcgctaa attctataac ctctccattt aagaaaaaag ctgtttcatc
     4381 tgatatacga gcagcttgtt gcatattatg tgtaactatg actattgtgt agtctttttt
     4441 taaatcctct ataagttcct ctacttttaa tgttgaaatt gggtctaaag cagatgtagg
     4501 ttcatccata agaataactt ctggtctcat agcaattgct cttgctatac atatacgctg
     4561 ttgttgacca ccagaaagtc ctagtgcaga tgatttaagc ctgtctttaa cctcatccca
     4621 aatagctgca ccttttaagc tttcttctac aattttatct aattgcttct tatctcttag
     4681 tccatgtgta cgaggaccat atgccacatt atcatatata ctcataggaa atggatttgg
     4741 tttttgaaat accattccta ctttagtacg aagatttata acatcatctg aggtatatat
     4801 gtcctctcca tcaacagaaa tgtttccttt aattgttaca tcttcgatta agtcattcat
     4861 tctatttaac gttcttataa aagttgattt tccgcatcca gatggaccta ttaaagcagt
     4921 aaccttattc tcttttatat ccatatttat tttctttaat gcttgcttat caccataaaa
     4981 taaatccaaa tcttttacac tcatttttat cttatcgatt aattccatta ttcacacctc
     5041 ttaaatttaa taattagctt tatttaattt cttagcaaca aatcttgcta gcatgtttag
     5101 tgctaataca attactatta aaacaattcc tatactggct gctgttgcta tatctccact
     5161 ctctttagtc agtaagtatg agtgtacagt taatgttctt gcactatcaa atattccacc
     5221 tggcatcttg gctacagtac ctgctgtaag taatattgca gctgattctc caattatacg
     5281 accaactgat aagattactc cagataagat tcctggtatt gcacttggaa gtactacctt
     5341 atataaagtt tgaaattttg tagctcctaa tgccaatgat gattctctat aagcttgagg
     5401 aactgttttt aaagcttctt ctgttgtacg aattattact ggtaatatta ttattgcaac
     5461 tgtaagtgaa cctgcaacta tggaatatcc catttttaat gtaactacaa agaaaattcc
     5521 tccaaataat ccatatacta tagatggtat ccctgataaa ctttcagttg caaatcttat
     5581 agctgtaact gcttttcctt ttttagcata ttcttgtaaa tatatagcag ccaatattcc
     5641 tataggagtt gctacaacta tagaaactac tactgtatat aatgttgtaa ttatcattgg
     5701 aagtatacca ccatcacctg atgctgaata attgctaaat aagaaattaa aattcatgtt
     5761 gccaattccc tttataaata taaatcccac tataatgact aatgatacta ttgtaaatag
     5821 tgctgataga tatactaatg acttcaatag attctctttg aactttctca tcttaattta
     5881 ccgccttatt tgaaagttta tttaaaacta agtttaaaat taatataaat gtaaacaata
     5941 ctactccagt tgcaaataac atttcttgat gtgtaccaaa tgcgtatccc atttcaagtg
     6001 ctatattagt tgtaagtggt cttacactat ctgttattgc agttggcata actggtgagt
     6061 ttcctgcaac aagtattact gccattgttt cacctaaagc tcttcctact cctaatacta
     6121 ctgcagctaa tactccagat ttcgcagcag gtagtacaac tttgaatatt gtttctattt
     6181 ttgatgcacc aagtgctaat gaaccttctt tgtatgcttt tggtactgct cttattgctg
     6241 tttctgatac agatattatt gttggtaaca tcattactgc taatactatt actattgcta
     6301 acaaactttg accttttgga agattaaata tactttgtat agttggaact atcactgcaa
     6361 gaccaaatac accatataat actgaaggta tacctgctaa aagttcaact gctggtgaca
     6421 ttatttttgc taatctttta ggtgctactt ctgctataaa tacagaggtt aatataccaa
     6481 caggtactcc tatcacaagt gctccaagtg ttgcaaatat tgatgctact atcatagatg
     6541 atattccaaa tttattggca gaaggaaccc aatctgcacc agttaaaaag tctataaatg
     6601 aatatccttc aaatataaat ggtcttaagc ctttataaaa tacgaatcct attatcaaaa
     6661 gtaaacttgc aactgctact aaagcactaa gtagaaaaat attttttgct actttttcaa
     6721 ttacgtattt tgtcttattg ccattattgt catttttttc taaacctact aatctagatt
     6781 ttactatcat aataccctcc cattaaaata tacttatcga ttttcgttct gtattcaaaa
     6841 tttaattttt tataattcgt cttcatcaaa tttcctgtac ttttattcta ctttttttaa
     6901 gttaaatcta tattaggatt aagttaagca agtgtaaaat tatatttact tttttaacat
     6961 taattttact tttaaaacta tatttgacat ttactttaca atatactgtt gtttctctat
     7021 attaaaatag cagatataga taataataca gtttatgtat aatttattac ctatatctgc
     7081 tattaagatt tgttaactaa atttaacttt tatactacat atataattat cgtatcctct
     7141 agtttatatc actattattt ctatcaaatt gttatgatac ttgatgtttg caataggtac
     7201 gaatagccaa aaatataaaa ataggtattt ctatcactga gaaaaacagt ataatgtgtg
     7261 gtgtccaaaa actcatacct cctaagtgta agaaattaaa tgatgtcatt tgatataata
     7321 aactaatctt aagaccaatt ccccctggtg gtaataggta tccaatccaa ctaacaccta
     7381 ttcccatagg aattaagcaa atagaaaaag caatcagcat agaagtaaga gaatccttac
     7441 attttgcaga taaaaataat gttaaactaa ccattgcaag tattgaaatt agaccaccaa
     7501 tagctataag aatctgtgct tgtagtaaat ttatatttac tagactaatg acagaaaaca
     7561 acatttgaaa tgaggttttc aaacattcag taccaaaagc taaatcagaa attgccaaat
     7621 ggatagaaat acatacaaca aacattacta taaatataga aaatagagct aatattttag
     7681 taaacgctag acgagtatgt ccatgtttcg tacaacgaag gatgctatct gattgtgtct
     7741 gatactcacc agaaaaagta ggtgcagtaa ttgcaacaca tataattaat aatataaaaa
     7801 tgtaaaatat tatataatcg aaagcatctt tagaataacc tgggtatatc tcaaatggag
     7861 tatctacttt tctataaagt tcttgagctt tcatttgtgc actggaagtt tgtcgttcaa
     7921 tcttcataat atcatttaaa tgagttttgc attgttcata aaaagaatct aaatcatttg
     7981 tatttatttc tgataattct gttgcaaatc ctgtttctga attagcaaat gcttcaggaa
     8041 gtctattcaa caatgggcgt attggtaaaa tacttttaga ataaatatct agtgggaaat
     8101 cttcactacc tactttacca tacttttctt ctgttttttt ccatatattt aaagcatgct
     8161 ttaatttttt tggtgttaca actccactac taatttcact attattagca taaattgttt
     8221 ttttatattt aatagcatct aaaccactca attcaactac cttgccatta ttatctaaat
     8281 aattgatacc ctcgtaagta attggaagaa aagccatcaa tgcagataaa accaatgcca
     8341 acaataataa aattaatgta cgacgtgagt gcaacactcg tttaatttcc aaataaaaaa
     8401 tacgcattta tttcccctcc ttactataaa ctgcttctgg aaataaccat aaatataaat
     8461 cttctagtcg tggttcaaca gcaatagagt tatcaatttg tgacttttct gccaagtaac
     8521 gaatagatac ttgattgttt ttttcactac gctgattgat tatacgtaaa ttcatttcat
     8581 attttgaaat atctctattt gatataagac attcccatac tttgccctct actttcttaa
     8641 ccagtttatc ggttgttccc acatcaagaa tttttccact tttcataata gcattttgag
     8701 ttgatatgta ctcaatatca gatacaatgt gagtagatat taggacaaca cgattatgag
     8761 aaaattctga aataaaatta cgaaatctca ctcgctcacc agggtcaagc cctgctgttg
     8821 gttcatccat tataaggatt ttagggtcat ttaacatggc ttgtgcaatt cccactcgtc
     8881 gtttcattcc accagataat ttaataatct ttttattttt tacatcagat aatgtaagaa
     8941 tttccaatag attattaatt tttcctttta cttctcttgg tggtacatct ttcaatgctg
     9001 ctatatattc taaataatct tttacagaaa aatcctgtgg gaaaccaaat tcttgtggta
     9061 aaaatcccag taaatttctg tactcagcac ccattatata aatatttttt ccattataca
     9121 gaattttgcc attggtaggt ttcataatgt ctgtaataat tctcatcaag gtagttttac
     9181 ctgctccgtt tgctcctaga aatccccaca cacctggtgt taaggtaagg caaacatctt
     9241 ctacagcaat tttatcctta aatttttttg tcacatgttc aatttttaat tccatatttg
     9301 aaactcctct ctttatctac ttacctgcaa aaacaaaagt gtcttgctta gtatttattt
     9361 tactaaacaa agacactcat aattttaata ttaaatagat aatttcttaa gaaaatccta
     9421 atatttgatt tatattttac taaatagatg taggaagcga tatagtaaat gttgtgactt
     9481 catctttact ttgaacagta atatcaccac catgcaaatt tataatttcc ttagctatag
     9541 aaagccctaa acctgcacca cccatatcag attttctagc ttcatctaaa cgattgaact
     9601 tttcaaatat agcagaaagt tgttcttctg gaattgtctg tccataattt ctaaaagcaa
     9661 tatctacctt gctgtcttta tgaaccgctg agataataat ttctgtatta ggatagctgt
     9721 aagcaatagc atttttcaag atattattaa acactcttgc aagtttttca gaatctgctt
     9781 ttattgttaa actttcattt gcttttagaa tggctctatt tcctttttca gacagcaatg
     9841 ggtaaaattc atcctttaat tgaagtaaca tataatatag gtctactttt tcgaaattaa
     9901 ttttgatttg attagaatta tatcgtgtta tttcaaaaag ttcattgata agtttttcta
     9961 aatattgtgc tttgtcaagt gcaacttcag tatacttaga tttttgagaa ttatccatat
    10021 taggtatttc ttttaataag ctcaaatacc caataactga agtaagtggt gtccttatat
    10081 catgtgctag atacatcaca aggttattct ttttattttc agcctctttc actgcttgtt
    10141 gacttaacaa aacagacatc ttaatttggt tcatttgttg ctctatttcc tttaatggct
    10201 tagatagttc aaccaattca tcattttgtt gataaatagt atgtgttgca ttgatgattt
    10261 ctcttaaata attccatggt tttttccaat aaaaattgaa aatagtgata tatccaatta
    10321 taaaaaccaa aaaaaataat acatctcttc tcaacttaag ccatttaaca agaggatttg
    10381 aaaaatttga aaacgtataa tcagccataa acattaaaac caatactata cctgtataga
    10441 tagctaacat tatatataat ttatacttta aatacgcctc tgtatttttt tctcttttta
    10501 taaatttata tttatctctt agtttcctat aagtaaaatt attaatcttt ttcataatac
    10561 tacatccctt cttgacaaga aaaataggtg tcaaatcttg taaccaacac cccaaattgt
    10621 ttttatatat tttgcttcat ctacagtatc acctaatttt tcacgaagat ggcgaatatg
    10681 aactgttata gtattgttac ttttattgta atattcatct ttccagatag catgaaaaag
    10741 attttctgaa tttaccacag agcctttatt tttcaataat acatgtagaa tagaaaactc
    10801 tgttggagta agtgaaagag ctttaccatt taaatggcat tcatatgttt gggtattcaa
    10861 ttccagacct aaaaaagaaa aaatatgtga aagttcttca ccagattgta aacttccact
    10921 gtatttctta tatcttctaa tctgtgcctt cactctagca acaagttcca aaggtcggaa
    10981 aggtttagta acataatcat ctgcacctaa agttaagcct gtaatcttgt ctgtttcttc
    11041 atccttagct gttaacataa tgattggata agtatatttt tctcggattt tttggcataa
    11101 agaaaatcca tttactcctg gcaacataac atctaaaatt gcaaggtcaa attcatttaa
    11161 attaatgcag tttagtgcat caagaccaga ataaaattta taaacaatat agttttcatt
    11221 tgtaaggtag acttctatta aatcagctat atcacgttcg tcatctacaa ctaaaatttt
    11281 tccactcata gactttaaac atcctttcta tatatattta tatatattat actaaattta
    11341 atttttttat atgatttttt acattagaat ttattaagaa attcttaatc ttattatgta
    11401 actattttat tatttaacat atattaaaga taaaaatgaa tatatataga tttagatata
    11461 caatttatct atcttttcaa atataatcta taaaataata gttctgttac gcattttttc
    11521 atataataaa actgactgta atttaactgg caaagtctag aagaataggc tttatcaatt
    11581 aaattacagt cagctatttt ataataattt agtttagata tcgttagcat ttcacattta
    11641 taacaattat taatgagatt aatatattta ttattcttta ttaaaataat atcctgctcc
    11701 tcttattgtt ataattttct gaggattttt ataatctttc tctattttct ttcttaatcc
    11761 attcactgca actaccacac tatagttatc ctctaagtac tcattcatcc atacatgttc
    11821 atatatctct ttagtagtca gagtcttttc aggattctct ataaaaaagt ttaacagctt
    11881 attttctgtt ggagtcaatt ttattatttt gtcttctaac tttattactc cactagttct
    11941 attatattcc tctaattttg tattattttc attattattt acatatgagt tcattcttct
    12001 taaaatagct tttacttttg catataatat ttctaaattt agaggcttta ttaagtaatc
    12061 atctccgcca gaatttaaag catttaaaat gctttgttct tcatttatat aagtcatata
    12121 aacaattgga caagtagtta catttctaat ttttttacaa agagtccatc catcaccatc
    12181 agttagaatt atttctaaaa atattaaatc atatttattg ctaaatattt ttccaatagc
    12241 ttcttcacaa ttatatgcta aatctatact tatttctttt tcctccagaa atccttttag
    12301 cttctggcaa ttatccttgt ctccatctat tatcaatatc ttagtttgca ttataatcac
    12361 tcccaaatat cattattctt ttatattaaa taagctatta ttataaaaaa catagactta
    12421 attttattgt aaaaacagtt ccttttccta gttctgaatc tacaaagatt tctccgccat
    12481 gctctcttat aatggatttg acaatatata accctagccc ttgccctttt ggctcattat
    12541 tatctctttc tgtaaagaat ctgtcaaata cctttggtat attttctgga ggtatacctt
    12601 ttccattatc agaaacttta ataattgcaa atccatcttc tttatccatt gataaaatta
    12661 tctttccatc aaatggagta aatgtcaaag cattataaat taaattctca aatgctcttg
    12721 taagcatctc tttatttatc tttgcatata tttttttctc caatatgtaa gtttcaaagt
    12781 ttacaccaca tgcttgtgta tctggcaagt tattttcata tatctcttta ataaaatcat
    12841 ttagacatat tttttcacta ataccaatgc ttttatcttg tgatgtaaat tcttgcaaac
    12901 tacgtactct attttgtact tcatttgact tttgatttat agcataaaga taggttcttg
    12961 tctcttcatc tacatgaata ttaccaacct ttatcaagtc tataaatgat tgtatagctg
    13021 aaacaggtgc ctttaaatca tgtgccacat ctgccaagaa ttttttacgt tcatttaaca
    13081 atgttgtaag ttcattagtt ctcacttcaa cttcatgttc aagattcctt gtaagttttt
    13141 cattctcttt tattattatt ctattcctgt ttatcatcat aagagaaaat aaaataacaa
    13201 ttataaatcc acaatattct gtttgccacc ctgtaaatat aggctcaaat ctattactag
    13261 taactgcatc aaataatatg ctaattccaa aaacagtgtt tactgaaact aaaatataag
    13321 aacttatatg ctcagagcat gtctttttat atattgaaac cagcgaagac attaatatat
    13381 atataaatac aatatattta tatatgtcta ttaatttccc atatatgttg acaaagtaca
    13441 tatcatatgg aagaagaata attggtatca ctatagatat gatactcatt gtaattccta
    13501 atggtaaaat taaaaatcta tacactttat cttcttctat tttggatata cttccattta
    13561 tacaaataat acataaaact accataaaat aggacgaatc ttcaaccgca tatgtaaagc
    13621 ttaccaaagg cagtcctaac aaatgtacaa agggatagga tacatggatg gcaaagaaaa
    13681 cacacataca tccaaaatat aagtaaagtt tacttttgtt tgaaagaaac catacagaca
    13741 acgaaaatat agctattgca aaagttgtga agcaaattat actataaaaa agtaatcgat
    13801 aaaacatcat attacttatg tcctttgttt ctcctaatgc aggcggataa tacaaaccac
    13861 tataataatg tgaaaaattc gatacattga ttataatttc tgtatctttc tttgcaataa
    13921 aacttactac tgaattttta attttgggtc tatatttttc tgttcctaca tctcctaatt
    13981 tagaaactat ttctccattt atccatactt cacttgcgga aaaaatctca ggaagctcta
    14041 aacttagtag cttatcctca ccttcatttt ttattctcat acgataagta gcattcccat
    14101 atggtgactg attccctttt ctcatagaaa aatctggata ttgacctata aaaacatatt
    14161 ctggttttat atcttgctct gtaaaatcat tcggtttaaa tattttatga ttataaaact
    14221 cccagtcatc tatcagaaag acaagtttac ctttagtgaa agtatcatta tctatagtta
    14281 aaattccatt acttgctcta atagacttat atgtatattt attatcatat ttatacagaa
    14341 gatttaatcc tgctattgcc aacagagaga ctatcaatac aatgagagtt ccttttagta
    14401 tattatactt tttcattgca tcaaccacat ctctttcata gtagtattaa ataaaacaag
    14461 aactatttat taattacagt atctcactta tggtacaaac ttatatcctt ttccccatac
    14521 ggtttgaatg tattgatgct ccctgcttaa agaattaatt ttttttctca tctgtgcaat
    14581 atgaacctgc actgtatgtt gatctccaat atcaggcatt ttccaaactt ggttatatat
    14641 ctctatcttc gaaaatactt gttctttgtt ttcagctaga aacaataata tttcaaattc
    14701 catagtagtt aaatctatac tttctccatc aaaagttact tttcttgaat ttacatttat
    14761 tactaacgct gaaaacttta acacattagg aactgatgta atatttttgt actgcctcat
    14821 acgagcatat attctaagtt caagttcttt taaactataa ggttttgtta tataatcatc
    14881 ccccccagct aaaaatcccc gaactttttc ttcttcatgc tcataattag ataaaaatat
    14941 tataggtatg ttaacctttt cttttatatc attgcacatt ttatatccat ctatatctgg
    15001 caatgaaata tccaaaatta tacagtctaa aactatacgc tctactatat ccatagcatc
    15061 tttagcagtc tctgatgtat atacattaaa acctatatta gaaaaataaa tttgatttat
    15121 atttaaaata tcaacatcat catctacaac cagtaaatta tacattacac cactccattc
    15181 aaaggtattt taatcataat ctca
//
LOCUS       DAEPOZ010000088        14992 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.088, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000088 DAEPOZ010000000
VERSION     DAEPOZ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14992
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.088"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            81..860
                     /locus_tag="KR440_001944"
     CDS             81..860
                     /locus_tag="KR440_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HBH0698995.1"
                     /translation="MNYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKVFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVVLGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDVVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
     gene            complement(1170..1991)
                     /locus_tag="KR440_001945"
     CDS             complement(1170..1991)
                     /locus_tag="KR440_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallohydrolase"
                     /protein_id="HBH0698996.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDITVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDADVLIISLVSDDGILNDGTKLEDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIITHIDEMWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
     gene            complement(2716..3534)
                     /locus_tag="KR440_001946"
     CDS             complement(2716..3534)
                     /locus_tag="KR440_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0698997.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNNTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDSVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            3702..4283
                     /locus_tag="KR440_001947"
     CDS             3702..4283
                     /locus_tag="KR440_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0698998.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            4280..4474
                     /locus_tag="KR440_001948"
     CDS             4280..4474
                     /locus_tag="KR440_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0698999.1"
                     /translation="MKIITIGSSLITVLLFLSTMVCGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYIKK"
     gene            complement(4638..6380)
                     /locus_tag="KR440_001949"
     CDS             complement(4638..6380)
                     /locus_tag="KR440_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HBH0699000.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEDLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQDGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            6809..8185
                     /locus_tag="KR440_001950"
     CDS             6809..8185
                     /locus_tag="KR440_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBH0699001.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            8496..9167
                     /locus_tag="KR440_001951"
     CDS             8496..9167
                     /locus_tag="KR440_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBH0699002.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            9358..10155
                     /locus_tag="KR440_001952"
     CDS             9358..10155
                     /locus_tag="KR440_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBH0699003.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
     misc_binding    10499..10733
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10783..11802
                     /gene="pheS"
                     /locus_tag="KR440_001953"
     CDS             10783..11802
                     /gene="pheS"
                     /locus_tag="KR440_001953"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBH0699004.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     gene            11819..14212
                     /locus_tag="KR440_001954"
     CDS             11819..14212
                     /locus_tag="KR440_001954"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBH0699005.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            14237..14827
                     /gene="zapA"
                     /locus_tag="KR440_001955"
     CDS             14237..14827
                     /gene="zapA"
                     /locus_tag="KR440_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBH0699006.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDVYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
BASE COUNT         5678 a         1766 c         2515 g         5033 t
ORIGIN      
        1 catctgaata gagggtgttt tatttgtaaa aaataagttt acagtttgag cgtataaact
       61 tatggtaaag gagaataatt atgaattata agaagattgc aattataacg ggtgctaata
      121 gtggttttgg taaagagttt ctaaagttat taataaatga agaagaaata acagaaatat
      181 gggcagttgc aagaaacaag gcgagattaa atcaattagt agatgaattt ggaagtaagg
      241 taaaagtttt ttcaaaagat ttatcaaaaa tagaagaagt aaaagaaatt ggtacatttt
      301 taagtaaaga aaatgtatgt ataaaatacc ttataaacaa tgctggtttt gcaaaattct
      361 gttcatacaa tgatttaagt atagatgaat ctattaatat gattgattta aatattagtg
      421 ctgttgtagt attgggactt atatgtattc catatatgca aaagggaagc catattatga
      481 atatttcttc acaagcatca ttccagccat tgccatatca aaatatttat agttcaacta
      541 aagcgtttat aagaaattat acaagagcat taaatgttga acttaagagt aaagggataa
      601 atgcaattgc tgtatgtcca ggttggatga gtacaaatct atttaagaga ggtatagttg
      661 tagctgaaaa agggacaaaa aatttcccag gcatagtgtc tccagacgtt gttgccaaaa
      721 aagcattgaa ggatgcaaag aaaaataagg atatttctgt gtatggtata aatacaaaat
      781 ccagtcattt attagcaaaa ctattgccac aaaaaatgat gatgaaggtt tggttaatgc
      841 aacaacatat taaggaataa gagtttcggt aattaagtat cagttggaga ttcaatctat
      901 tatatattat aagaagtttt gttgagcaaa taataattgt gacaaaacgg cttatctagt
      961 gccgataagc cgtctataat gtataaaaaa tatatttttg attttacttt atttagatat
     1021 tgaagtatta aaatatgtag gacttgaaat tttataaaga taaatactat gatattttgt
     1081 aaaaactgag atatgttgat attacatagt ttaactatac tgttaaattg aagttttctt
     1141 attttgcatt atgcttatta cttaatgatt tagactataa tttccatacc atcatatgca
     1201 aaataaatgt tatcaagttt cttttctaat tcactgtaat aaccatatga ttttccccac
     1261 atttcgtcaa tatgtgtaat gataatcttt ttaattctat aatagttttt aatttcaaca
     1321 atctcatcta aagtaaatat ttcatcttta aatggtgtgt cttcaagttt tgtaccatca
     1381 tttaatatac catcatcaga aactagactt attattaaaa catcagcatc aaagtacata
     1441 tcactatgag taaatggctt gggattacag caggcatata tgactttttt ttcattctct
     1501 ttgataacat agaaggttat attcctgtct gggttattgt taatcaaatc aatatcaaaa
     1561 ttatttatct ttacatgcga agtttcatta atagaaatgc aatttataac atcaccataa
     1621 tatttaagac aatcgagatt cgttcggttt atatcttcaa ttacttcagg aagagcataa
     1681 aagccaattg gattattttc tcctgtgata aagtcacatc ctattttttc gacaattcta
     1741 atacccttaa catggtctgg gtcatggtga gaaattgaaa tatgttttac ttccgatatt
     1801 ttttgtctgt tacaagctac tgtaatatct tcaggtgtgt ctatcagcat ttttatatca
     1861 tcaatatata aacttggtcc taatcgttca tatcttccgc ctttttgcct ggcttcttca
     1921 catattttgc atttacaaaa tgaacttgga ataattgaca taccgccact accaacaatt
     1981 tttaatttca aagttattac accaccttct aaaaatatta gattatataa ataatctaca
     2041 atcacaactg attaacaaaa ttatgagtta ataaattata tcacatgtaa atatgaaaga
     2101 atagtatgaa atttttataa gattatatta aatatttctc aatgaaattg actttgtata
     2161 tgagccgaat gactttattc aatatagata agacattttt cttgtaataa gatatgatta
     2221 ttctataagg ttcatgtaat tttgatataa aatattgatt aataacatat atgttattgg
     2281 tgaacatatt tctatagaat ggaatatata attttttata tttatcattg tattattata
     2341 caaagaaagt taaaaagagt agagtacaaa ggaattattt gtactttact ctttgttttg
     2401 ttaaaaacta atttatatta gtgattcaaa tatttaacta gagtataagg gttgtatata
     2461 atcttataag taatttatct gacaaattaa gataatatcc taatcaaatt ctagtcattc
     2521 gaacataaca tcacaagtga tttatctgac aaattaagat aatatcctaa ttaaattgta
     2581 gatatttata cataacacta aaagtaattt gtttaacaaa ttaagataat atcctaatta
     2641 aattgtaggt atttatacat aatactaaaa gtaatttgtt taacaaatta aaatattatc
     2701 caattaatat aaaaattact tctttttaac tgggaagcat acctctgtta cccaattatc
     2761 aggattacta tcgttgccag gacttacatg ataaatatta aacataggac catccacatc
     2821 atagttatta tctgatatcc aattaccaat agcctcacaa gcgtctgcaa cttgattgaa
     2881 atttccatta acaattgcag ttgcagcagt cacacttgga acagtcttaa atttaacatg
     2941 ttcagtatct ttatactttc cagatacagc aacttgtact tcaacatcta cagaatcttc
     3001 tttatatcca gtatcataaa agactgcttt agaatacttt gggggttcaa tttgaacatt
     3061 ctggtctttt gtctctaaaa atgcttgatg ccataacatt ccttcgtctt gataagttgg
     3121 tataaccctt ctaagtgtca tcatatattt tggtgcaaaa ttctttatag ttacatcata
     3181 atttttcata atatcatttc ctccaattct tttaatagtg gtttctattt ttaatagttt
     3241 tttctgtgta tcttctaatt gttcttttac ttgagaatgg tggatattta gatattttat
     3301 aagactttct ttatcgttat attctgtcaa aatttctttt atagaataca agccaaagcc
     3361 catatctttt agtgcatgga ttctatttgt agtagaaagt tggtctgcac tatagtatct
     3421 atatccatta gtgttattcg tatgagaagg agttaaaagt cctatttcat cataatgacg
     3481 taacatacga atactaattt tacttagctt tgaaaaatcg cctatcttaa acatgttttt
     3541 gtctccttta taaaggtact tttagcttaa tattaatata aaccatatca taatgttaga
     3601 gtcaactaaa attataatta aattatattg acatatgatg aaaaaaatat tatactgaac
     3661 atgttcagaa cctaaaatgt taattatttt ggaggtctat tatgaaaagt aatgataaag
     3721 atgctagaga acgtataatt gaagttacat tgaatctttt aaatgaagta gatgatattg
     3781 aggagattac agttagaaaa attgctgagc gtgcaaatgt tggagttggt ttaataaatt
     3841 atcattttaa aacaaaggat aatttgttga gtactgccat aggagatgtg atgtcaaata
     3901 taatagcaga gctatatgac gatagtgtat atactctaag acctattgaa gatttgaaaa
     3961 atttattaaa aaaattgtgt gatactggat tacattatga aaaagtgctt ccttttgtgt
     4021 tgaatcaatg tataaccaat ggagatatgc aagcagaact tgatattgtc cctatgcttc
     4081 gtaaaatttt tggaaataaa aaagatgaaa tgagtctaag aatcattgcc ctgcaaatta
     4141 ttttaccaat acagatttct gctttatcta cagaatcatt tcagttatac agtggtataa
     4201 atataaagaa taaatatgag cgagataaat tcattgatat tttaatagaa aatattattg
     4261 gagaggatgt tgatgtaaga tgaaaattat aactattggt tcaagtctta tcactgtttt
     4321 actttttctt tcaactatgg tttgtgggtt ctggattaag aataataagg taacagatgc
     4381 aagttctatt aagttccata tgaatagtgc tatttttaca gggatatttt tattgatttc
     4441 cacaatcctt ttaattatat atataaaaaa ataataatat tattaataaa taaagtatca
     4501 gaaattataa agacaagagt cttaaaaatt tctgatactt taccacataa ttttattaaa
     4561 cattaattgt gtgtgaaaaa atatcactta tttcaatcta atactaaatc ttaagagtga
     4621 gtataaagta ttagaaatta agaagcattt tttagttcaa gtggattatc tataccaaaa
     4681 taatcatata aagtagagct tatttcctct aaatttccat cttggaaagg taattgtaaa
     4741 tttacctttt ctaccacatc tttaaatcca gcatcagtat tttgatttaa aaaatccata
     4801 tatacatcaa cagcttttct gtaatcttct tgtgataatt gccatatctg aagtgataca
     4861 tcagatgcaa gaccataact caagtaataa aaaggaactt ggaagttatg agatacaagt
     4921 atccaaaaag gagcattttt ttctctaagt atattattgg aaactccata ttcttcctct
     4981 aaatcaaaaa acaatttatt gatatcattt agactcatat atggattctt gtatataatt
     5041 tcttgaaatt catcataaag acaagcatca ataatagtat ttaaaacaat cgataagtgt
     5101 tcttttttta tagcttcaga ttgtttacca aaaaaatcat caaaatattt ataaaataga
     5161 atttctaaag atgttgaatg aacttcacat acatctatac ttggttgcat cttattattt
     5221 aagttaatac tgttgtagta attataaaaa tgaccaaact catgagcaaa actagtaaca
     5281 cctaaaaaag aattgtccca tgtattaaac aagaaaggtg ctttatattt atttatataa
     5341 gtagtatatc ctccaggcga tttgtttttt ccagaagaat aatcgtacaa gtcatatttt
     5401 ttcatatagt cgaagctctc ttttagtttt ggagatatat cttcaagtac tttatcaaac
     5461 ttcctaaatg agcgattttt atatacttta atataaatag aagaatcaga tggtttagat
     5521 gaaatctctc tatataatgg aactatgtaa tcttttacat cttgtctaaa ttttttagct
     5581 tcttcttgag aatagtcttt gtttaggttc atataggcat aatctatata attcttaaat
     5641 ccttgcttac ttgctatttc agttcttgtc tgtataagtt ctaagaatat gttaccaaat
     5701 ataggattgt atttttttaa gtaatctgaa taaattttca catattcttc agggctaaga
     5761 tttggtcttg acaaggcttc ttcaaagtct acctctatac catctattga tactgtagat
     5821 ttagctaata agtcttcata ttttacaact aggtcttgct cttttttaag taaatcttca
     5881 acttctttag aattaagttt tccaatgttc tttagataat taaatgtatc ctttccaagt
     5941 tctttttcta attctttaga gaatttagaa gttacaaatc tatcttgaaa gtctttgtat
     6001 gacatgtcaa catctactga tttactcata agatacttat attccttaga gtaaaattcg
     6061 tctgatgaat ctatgttatt tctaattttt gctatagtaa gtgtactgta aaaatcctga
     6121 taaattttat caacttcatt aaataaattt aattgttcct tagctgtttt agctgttaaa
     6181 agtttgtcgt tacacttgtt aatgttttcg caaattgact gtatattagg acgcttatat
     6241 tccatttttt caaaaggaat agttcctcta acagaatctt tttttgtatc attagtataa
     6301 tttcttatgt atgatgcaaa tataataaaa actgcaatta aaattagaaa aacaaatatt
     6361 ttcttcttgg atttaaccat aaaaatcctc caatcatgga tatagttatt aattatttta
     6421 ctatattaaa tataaaaaat gcatgttaag aaaatttaac ataaagatat agataagatt
     6481 ataaataata taaaaaattt aacttcaaaa aagtatatga aatttatact ggtaaaatga
     6541 taaataaatt ttaagtacaa aatagtaaaa gctatgatag ttttagaccc attatatgta
     6601 aaaaatatag atgtttttgg tgcaatttaa attaaaagat atatataaaa atttaaaata
     6661 ttccaaacag aaattataaa aattactatc attgtcttta tgcaatattt atactataat
     6721 agaataataa tttagaggaa agctctaaaa ataaacaaat taagggtctt aacagtagtg
     6781 attttatgtt aaggaggtat aaagaaagtt gaaaacaata gttagaagac ttattctcta
     6841 cgcagatgga atgcgtccta ctcaaataat ggtatctggg tttgctgcga ttattgtaat
     6901 cggagcattg ttgttaacac tcccgatagc gtcgcagagt ggagagagta taggcttatt
     6961 aaatgctcta tttacggcta cttcagcggt ttgtgttact ggtctagtta tggtagatac
     7021 agcaacttat tggagtttat ttggacagat agttataata acattgatac aaattggtgg
     7081 tcttggattt atgaccgtag ccacaatgtt ttcacttatg gcacgaaaaa aaatacaact
     7141 aagagaaaga ttattaatac aagagtcatt aaatcaagct gatttatcgg gacttgtaag
     7201 acttacaaga tttgtattaa taataaccat aacaatagag ggaataggtg cactggtttt
     7261 atcaactgtc tttattccac aatttggttt gtctaaaggg atatggtata gtgtatttca
     7321 cgcaatatca gcgttttgta atgccggatt tgacttgatg ggcagtgtaa gtggcccatt
     7381 tacatcgtta aattcttatg taaacaactt tacagtatca atgactgttt gcgcacttat
     7441 agtgcttgga gggctaggat tccctgttgt acttgatatt gtaagaaaaa gaagattttc
     7501 aaaattaaat gtgcattcta aggttgtatt gttttcaact gctacactta tttttgtggg
     7561 tgcattattt atttttctta tagaatttaa tcaaaaagct actatggcgg acttgccttt
     7621 aaagggaaaa gtactatcag caatatttca atcagtaaca gcaagaactg ctggttttaa
     7681 tacacttgat ttagctactt tacgagaaag tagtgtattt gtaatgataa ttttgatgtt
     7741 tattggagca tcacctgcat caactggtgg gggtataaaa acaacaacac tagctgtttt
     7801 aatcattaca gttagaagtt ttctatcagg aaaatcagat atagaagctt ttgaaagaag
     7861 attagctcca tcaactataa aaaaatctct tggtatattt gtaatcagta tatcagcagt
     7921 tatttttgga acattgatta tttcaataac tcagcctaat tttacattag ttcaatcagc
     7981 ttttgaggtt acatcagcac ttgctacagt tggttcaagt ttagctggaa ctccaaattt
     8041 aaatgcttta ggtaagatta taataataat ctttatgttt atgggtagag ttggttctct
     8101 tactttattt atggctatac tttctggtgg tagaagaaag agtcaaccaa tcagatatgc
     8161 agaaggtaaa attatggttg gttaagttac caaggagtaa tataaattta taatggattt
     8221 agagtagcac tgtaaatctg ttgataaaat aaatgagcct agatgtaatt atatgaagaa
     8281 ataaaatcaa ctaataaaca aaattaatta atgaaataaa aaaacaggga aataaaatac
     8341 aaataaaaag gtaaatcaaa attaaattaa tctgtgtata taaaattata tattgtgtat
     8401 aagttttgta tgtgtaaagt atataagttt tgtatgtgta aacaattaat ttaagagata
     8461 ttatataaat tgtgtattag ggaggaagtt tcgagatgaa gcaatatata gtcattgggt
     8521 gtgggagatt tggaagttca gttgcgtcta ctatgcatct tttaggacat caagtaatgg
     8581 caatagacaa aaatgaagat tcagttcaaa gtatatctga caaggtaacc cattcactta
     8641 tagtggatgt tactgatgag caagcgttaa ggtcattagg tttaggtaac tttgatgtag
     8701 cagtagttgc aataggttct gatataaggg catctataat ggcgactctt atagccaaag
     8761 aaatgggtgt agagttgata atatgtaagg caaaggatga attacaagct aaagtgcttt
     8821 ataaaattgg cgcagataga gttgtatttc cagaaagaga tatgggagta agagttgcac
     8881 acaatttagt ttcggataat atattagacc atattgaact tgacccagag tattcaattg
     8941 ttgaaatcgt aactccaaat agttgggttg gcaagacact tatagagctt gaattaagag
     9001 ctagatatga gataactgta cttgctataa aaacaggtaa aaatataaat gttacacctt
     9061 ctccagatga ggaacttaca gccggaagta tcctagttat aatcggtcaa aatactagta
     9121 taacagcgat aacatctgga aataagggga taattagaag aagataattt actatttaat
     9181 atatatttaa ttgcaatgaa agtaaagagt atcatataat tatgaatagt tatatgatac
     9241 tatttttatt taatcgaagg tagtatttat ttgtaagatt agataaagag aagttaaatt
     9301 aaaagtaagg aggctgtgct aatataaaaa tttataagtt attagcattt agataaaatg
     9361 attacaaata taaacagtaa ggataatgaa aagttaaagt atacaagagc actattaaaa
     9421 tcaaagaata ggaataaaga gtcaaagttc ataatagaag gatacagaat agtaatgctt
     9481 gcacttgaat gtatggcaaa ccttgattat gtatttatca atgaagaatt tgaaaataag
     9541 aaagaacatg taaaactatt agaagatttg gataaaaaaa acacaaagat atacaagact
     9601 actaataaaa actttaaaga attagtggat acagaaaata ctcaaggaat aataggtgta
     9661 gtttcattta agaaaaaaaa attaagtgaa agtataaata aaaaagataa atttgtattg
     9721 attttagata gaatacaaga cccaggaaat atggggacta taataaggac tgctgattct
     9781 gctggagtag atgccataat agcactaaag ggatgtgtcg atatatacaa cccaaaagta
     9841 attaggtcta ctatgggttc tatttttgat atgaatataa ttgatgcttc acaagatgaa
     9901 actgtggaca tgcttaaatc attggatttt aatatagttt caagttactt aaatacagaa
     9961 aatttttatg acaaaataga ttatggttca aaagtagcat tggtgatagg aaacgaagca
    10021 aatggaataa atgaagaact tgtatcaaag tctgatattt tggttaagat acctatatat
    10081 ggtaaagccg agtcgttaaa tgctgcgata agctctgcta tactgatgta tgaaataaaa
    10141 aaatacttaa tttaatgtat tgtaataaat ataatgtcat gttataatct tgaattaaat
    10201 agtagagtat caaaaatagt caatatatat taaaaaaata attaaattaa ttatatatta
    10261 gatgtatatc atataaaaaa tgattgagtt gattatgtat tagatatata ttatataaaa
    10321 aataattgag ttaattatgc attagatgta tattatataa aaaataattg agttaattat
    10381 gcattagata tatattatat aaaaaataat taaattaatt atgtattgga tatatattaa
    10441 aaatagtcac ataatttgaa tggaaatgat ataatactaa aataaacaat ataatattgt
    10501 aaatgcaatg aaagaggaaa gtattttgat taaacttagt aaagagataa acacctaggc
    10561 tgggagtgtt ttctaagagg tcatgagaag ttccctctgg agtaacagag ctgaaatttt
    10621 acagtaggct ttgacgtcaa aaacgcgtta agtttgttag aggtggtttg atgattttta
    10681 aattgttaaa ctactagggt ggtaccgcga aactatagcc tttcgtccct agacagggat
    10741 tgaggggctt tttttataca aaaaaacgaa agggtgatat gtgtgcaaga aaaattactt
    10801 gctttacgtg aagcagcttt ggctgaaata aaagaagcac aaagcataga aagtgtagaa
    10861 agtttaagag ttaagtactt aggaaaaaaa ggtgagataa ctgccatact taaagaaatg
    10921 ggtaaattat ctgctgaaga aagaccagta gttggtaagg ttgccaatga ggtaagagaa
    10981 aacattgaac ttagcataaa ttctaaaaaa gaagaaataa atgctattga aaaagaaaga
    11041 aaattaaaag aggaagtgat agatgttact caaccaggaa aagttttaaa ggttgggaag
    11101 aagcatccaa taactcaaat tatagatgaa gtaacagata tatttatcgg aatgggattc
    11161 tctatagcag aagggccaga agttgagact gttgaaaaca actttgacgc attaaacgct
    11221 cctaaagacc atccatcaag agatatgagc gatacattct atatcaatga tggggtatta
    11281 cttagaactc aaacatctcc agttcaagta agaactatga gaagtcaaga gttaccaata
    11341 aaagtaattg caccaggtag atgttttagg tcagactcgc cagatgctac acactcacca
    11401 atgttccatc agatagaagg gcttgttgtt ggaaaagatg ttactatggc agaatttaaa
    11461 ggaactatgg atatcttcgt tgaaaaattg tttggttctg atatcaaaac taagtttaga
    11521 cctcacaact tcccatttac agaaccaagt gcagaggttg atgttacttg tttcaaatgt
    11581 ggtggtaaag gttgcccaat gtgtaaatat gaaggttgga tagaaatatt aggttcaggt
    11641 atggttcatc caaatgtgct tagaaattgt ggaatagacc cagaagttta cagtggattt
    11701 gcatttggag ttggggttga aagacttgca atgcttaaat acgaaataga tgatattaga
    11761 ttattattcg aaaatgatat gagattctta aatcaatttt aattaggagg gtatgtagat
    11821 gttagtatct ttaaaatggc ttagagacta tgttgatata gacatggatg taaaagagtt
    11881 cgctgataaa atgacaatga caggaactaa agttgaaaca atagattatt atggtgaaga
    11941 aatagaaaat atattggttg gaaagatttt agaaataaaa caacatccaa atgctgataa
    12001 gttggttgta actaaagtag atattggaga taaagttgtt caaatagtta caggagctac
    12061 aaatatatca gaaggagatt atattccagt agctgtaaat ggttctaagt tacctggagg
    12121 agttgaaatc aaacagactg atttcagagg tgaattatca gatggtatga tgtgttcagc
    12181 agctgaacta ggtatagatg aacattacat tgaggagtat aaaagaggtg gtatatatat
    12241 tttagaccac gaagattctt atgaattagg aaaagatata aaagatgttt taggattaaa
    12301 agatgcttta atagattttg aattaacttc aaacagacct gattgtaaat gcatgatggg
    12361 tatagctaga gaagcagctg caactatagg aacaaaagta aaatatcctg aaatcgaagt
    12421 aaaagaaagt gacgaagaga tagatttcaa agttgagata gataatccag atttatgtag
    12481 aagatatgtt gctagaatgg ttacagatgt aaaaatagaa ccttctccat attggatgca
    12541 aagaagactt acagaagcag gagtaagacc tataagtaac atagtcgata taacaaactt
    12601 cgtaatgtta gagcttggtc aaccacttca tgcttttgat ataaatcaag tagagactgg
    12661 aagaatagta gtaagaaatg ctaaagatgg agagaaactt gtaacattag atgatgttga
    12721 gagaacatta gataaagata tgctagttat aacaaatgga gaaaaatcac ttggtttagc
    12781 tggtgtaatg ggtggtgcta actcagaaat aacttctaat acgaagactg tactttttga
    12841 aagtgccaat ttcaaaccag aaaacataag aatgacagct aaaaaagttg gtattaggtc
    12901 agaagcatct tcaagaaatg aaaaagactt agaccctaat cttgcagaga tagcagcaaa
    12961 tagagctgca caacttgttg aaatgttagg agcaggaaaa gttttaaaag gtgttgtaga
    13021 tgtatatcca aataaaccag aacctaaaaa attggtagta aatcctcaaa gaattaacca
    13081 cctattaggt gtagatgtac caatggagca gtttgtagga attttagaat cattagagtt
    13141 taaatgtaat ttggtagcta atgataaatt agaaatagat gtaccaagct ttagaacaga
    13201 tatggaacaa gaagctgatg tatgggaaga aatagctaga atttatggat ttgagaatat
    13261 accttctgta caattagaag gaaatacgac agcaggaatt aaaacttcaa aacaaaaatt
    13321 catggatgct ttaaaagata attcaactgc tgtaggatta aatgaaatat taacatattc
    13381 atttgtaagc cctaagggag tcgataagat aagagtacca gaaggtaatg ctaagagaaa
    13441 ctttgttaaa ttacttaacc cattaggaga agaaacttct gtaatgagaa ctactttaat
    13501 accaaatatg ttagatgttt tatcaactaa tgtatctcat aaaatagaag aagtatctgc
    13561 atttgagtgt ggacatatat tcatcccaca agattcagaa ttacctaaag aagaaaatag
    13621 aatgtgtgta ggtatgtatg gtaaagatgt tgacttcttt gcactaaaag gaactataga
    13681 aactatactt gtaaatgtcg gatttaaagg ttatgaaatt gaacctcaag ataataatac
    13741 taccttccac cctggtagat gtgctaaaat agtgtataat aataaatatg taggtacatt
    13801 aggtgaattg catccagatg ttatagaaaa ctacaatcta ggtcaaagag tttatgtcgc
    13861 tgaaatagat attgactttg tatttgataa ttctgataga acaaagaatt atgtgccatt
    13921 accaaaatat ccatctacat caagagatat agctcttatt gttaaagatg atgtatttgt
    13981 aaaacaaata gaagatataa taaaggaaaa tggtcaagga ttagtagaaa gctacaagtt
    14041 atttgatgtt tacaaaggtt ctcaaataga agctggatac aagtcaattg cgtattcaat
    14101 aacttataga agtaaagata aaactctaac agatgaagat gtagctaagg tgcatgataa
    14161 gatattaagt gaattaagcg aaaaattaaa tgctaactta agatcaaatt aacaactaat
    14221 gtcgggggag gttaggatga acaaagtaat ggttaaaatc catggcgctg aatatccaat
    14281 ggttggagac aaatcagaga agtttatgat tagtattgct gattttgtag acaaagaaat
    14341 ggataaaata acacgtcaaa acccgaaatt gagcttatca gttgctgcga tactaactgc
    14401 attaaatata tcagaccttt tatttgaatg ttcagatgag aatgagaagt tgattaaagc
    14461 taatgaagaa ttgagtaaga aggttggagc atctaatgaa gagcttcaat tagaaatcaa
    14521 gtctttaaag cttacgatag cagaaaaaga agcagaaaat cgtgaagctg agactaagat
    14581 gaaagagtta attgagatta tagaaaataa aaagcaagaa atattcgaac ttagtaatac
    14641 gacagaaggt tctagagcag agttagatgt ttataaaaac aagattgagg agttaagtac
    14701 tcaacttgaa gaagcaaatg aaagagctac tatagctgaa aacttagctt ctgagtttca
    14761 aaacaaagct tatgatttac agttaaaatg tacagggtta aataatgatg caaaaaatgt
    14821 ggaataaact aaaagatttg ctgtctcttg ttttttgaga cagcttcttt taatgtataa
    14881 atataaatgc tatataaata tagctaatgt ttaaatgtaa ataaatataa atgctatata
    14941 aatatagata atgtttaaat gtaaataaat ataaatgcta tatagatata ga
//
LOCUS       DAEPOZ010000089        14964 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.089, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000089 DAEPOZ010000000
VERSION     DAEPOZ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14964
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.089"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            538..1449
                     /locus_tag="KR440_001956"
     CDS             538..1449
                     /locus_tag="KR440_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBH0699007.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            1624..2121
                     /locus_tag="KR440_001957"
     CDS             1624..2121
                     /locus_tag="KR440_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBH0699008.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNAIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVILAGKYAKFVIVGGQKEVGDFWFKFWQMDFDRTYISDFEEYQCNTFDTKKQEIHI
                     YIGIK"
     gene            2865..3227
                     /locus_tag="KR440_001958"
     CDS             2865..3227
                     /locus_tag="KR440_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBH0699009.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVNNVLK"
     gene            3293..3910
                     /locus_tag="KR440_001959"
     CDS             3293..3910
                     /locus_tag="KR440_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HBH0699010.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            3907..4392
                     /locus_tag="KR440_001960"
     CDS             3907..4392
                     /locus_tag="KR440_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HBH0699011.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            4435..4953
                     /locus_tag="KR440_001961"
     CDS             4435..4953
                     /locus_tag="KR440_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HBH0699012.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            5089..6048
                     /locus_tag="KR440_001962"
     CDS             5089..6048
                     /locus_tag="KR440_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBH0699013.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNTRTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            6074..7777
                     /locus_tag="KR440_001963"
     CDS             6074..7777
                     /locus_tag="KR440_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HBH0699014.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINRV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            8034..10136
                     /locus_tag="KR440_001964"
     CDS             8034..10136
                     /locus_tag="KR440_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBH0699015.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESTDEKEAIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKNTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKNVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQINEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMHGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            10149..10589
                     /locus_tag="KR440_001965"
     CDS             10149..10589
                     /locus_tag="KR440_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBH0699016.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            10625..11428
                     /locus_tag="KR440_001966"
     CDS             10625..11428
                     /locus_tag="KR440_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBH0699017.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDKTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKSFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            11468..12064
                     /locus_tag="KR440_001967"
     CDS             11468..12064
                     /locus_tag="KR440_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HBH0699018.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGANGLLKMKENGLFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            12170..13033
                     /locus_tag="KR440_001968"
     CDS             12170..13033
                     /locus_tag="KR440_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HBH0699019.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            13100..14089
                     /locus_tag="KR440_001969"
     CDS             13100..14089
                     /locus_tag="KR440_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBH0699020.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NRESREKFAEEMNLKLSKYNTKIVAVESSDEAIDNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            14181..14792
                     /locus_tag="KR440_001970"
     CDS             14181..14792
                     /locus_tag="KR440_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBH0699021.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
BASE COUNT         6138 a         1392 c         2751 g         4683 t
ORIGIN      
        1 agaaaataaa taatatgtaa agactttaaa ttaattttaa gtattaagta caaatactaa
       61 tttttgatat agaaattgaa taaagtaaat gataataagc aaatgaatta agatgataat
      121 aagtgaattg atatagaaat aagtaaatta agtaagatga taacaaatga aatgataata
      181 agtgaattga tatagcaata agtaaattaa gtaagacgat aacaaatgaa atgataataa
      241 gtgaattgat atagcaataa gtaaattaag taagacgata acaaatgaga tgataataag
      301 tgaattgata tagaaataag taaattaagt aagatgataa caaatgagat gataataagt
      361 aaattgatat agtaataaat aaattaagat gataatatcc aaagtgagaa agataataga
      421 aagtaaataa aatcatgtaa gaataattta gataagattc taagatttta tttatttaaa
      481 aattagtatg attaatttag attaaataaa tttaataatt ttagtaggtg gtaatgaatg
      541 aaaataaata gattaacaga aataatagta attctattaa ataaaaaatt agtaacagct
      601 aaagagttgg cagataggtt tgaagtttct acaagaacca tatatagaga tatagaaact
      661 ctgtctatgt cgggagtacc agtatatatg acgaaaggca aaggaggagg gatatcttta
      721 atagaagaat attctataga caaggccata ctgtcaaaaa aagataaaga gagtttaata
      781 gttgcactga aaacattaca ggcgactaag tatccagaaa taaattcagt agtaaataaa
      841 attggctcaa tatttggaga gcagaatttt tcaaactgga ttgaaataga ctttactgaa
      901 tggggaagta attttaatga agatgataaa tttacaaaaa taaaagaagc tattttaaga
      961 cgtaatacta taaattttaa ttatgtaaat agtttgtcaa gccagacaaa cagaactgtc
     1021 gaaccactga agcttatgta taagagtaag acttggtact tgtatggatt ttgtaaacta
     1081 aaagatgatt ttaggatatt tagaattagt aggataagga atttatctat aaaggatgaa
     1141 gtattttcaa gaaagataat agaagaagta tgtttgaatg attctaaagt aattaaggaa
     1201 aatactataa cgttgaaatt gaggtttaaa gaaaagatgc tttttagagt atttgatgat
     1261 tttaataagg atttaataac taaaaatgaa gatgatacat atgatgtaat tacagaattt
     1321 cctattggtg aatggattta tggatatatt ttatcgtttg gtgacaatgt tgaagtactt
     1381 gaacctaaag atgttaggga taatgtaata actagactaa gggagttgtc taaaatttat
     1441 tcattataat atgatgctag attaaagatt ttattaaata tataatatct tgcatttagt
     1501 atataatatc tcactgagtt taattatatt aaaaatatga cataaaggtg tcgtatatta
     1561 taaattataa ttgaattagt tcaaaataaa atatattaat aaaaacaaag tgaggtataa
     1621 agtatgaatt atgaaatagt agagataaat gagaaaatag ttataggaat atcgaaggag
     1681 actacaaata aagatggaca agcggtaaat gctataggtg aattgtggaa aaaatttatg
     1741 ggcaaaggga tatataatgc tataaagggt aaaaagaatg acaagacaat aggtctatat
     1801 acagattacc agggtgattt tacaagtccg tatagctttg ttgcatgttg tgaagtaaat
     1861 agtaattctg ataaagagaa gaatttagaa gaattaaaca ctaataatac tgttggagaa
     1921 agtattatta gtaaagttat actagcaggt aagtatgcaa agtttgttat agtaggaggt
     1981 caaaaagaag taggagattt ttggtttaag ttttggcaaa tggattttga taggacttat
     2041 ataagtgatt ttgaagagta tcaatgtaat acttttgata caaagaaaca agaaattcat
     2101 atttacatag gaataaagta gatttgttat tactatattg aatgaagtat tgagtatagt
     2161 acatttatta tagatagttt tactatttat aactataagc agtaaatctg taaaagattt
     2221 actgcttttt tagtaattaa tagtatcagt atcactttga atcttcagac atattcgtca
     2281 tttaatattg tttattgata aatgatactt gtaaatataa tgatttgtta tttaatttaa
     2341 aatatgaaaa aaagtattat aaacacatta gaatacttct ggatttatga gtatacaatt
     2401 tgaaaaatat aaaaaataat gtataaagct aaatctaatt taatcgataa tacatataga
     2461 gaaatataat taagtaaata aacaactatc tattagatat tgagaaagtc aaatcctata
     2521 tgaatctaca gaaatgattt taatgaatct gaaaaaattt tttatacaaa cataaaaaag
     2581 gtataaagtt gttttattat aatttgatta cttttgataa ctttttatgt tttatacaat
     2641 tatattttta ataactcgtc aaaacgccaa acatatatta caatagatat ataagttttt
     2701 ttgttgacaa attatgttaa gagaattaag tataatatgg cataaataat tacttttatt
     2761 tatcaatgaa atgtatttat tgtgtagcga aaaatagtca gatagtatag acaataactg
     2821 aagataaaag cgtttattgt tgtacatatg gaggagttag ttttatggaa aataaaattt
     2881 tgatagtaga tgatgctatt tttatgagaa tgataattaa agaagctctg agtaaaagtg
     2941 gctataataa tttaatagaa gcaaatgatg gagaagaagc gtgtaatata tttaaatatg
     3001 aaaagcctga tttgatgttg cttgatatta caatgccaaa aaaagatggt ttagaggtgc
     3061 taagagaaat aaaaaagttg gattcaagtg ctaaggttgt aatgtgctct gcaataggac
     3121 aagagaatat gattgtagaa gccataaaac ttggagcgtt agattttata gtgaagccct
     3181 tcaaaacaga aacattagtt aaagttgtaa ataatgtttt aaaataaaag tgtaaatata
     3241 cactgataca atgaaaagca aataaatgca ataataaaag ggaggggttt atattataaa
     3301 ttatttagaa cttgatgatg ttcatataga tgctcttaga gaaattggta atataggctc
     3361 tgggaatgca attactgcac ttgcatccat gataaatagc aatgtagaag tattaatacc
     3421 tatggtcaag atacttgaat ataatgaagc gacaaatttg cttggtggtc cagaaaacaa
     3481 agtagtatgt attttactcg atatgaaagg tgatatcaat ggaatgttta tgtttttgtt
     3541 ggatgaatca ataactcaac ttatgctaag ttctcttttt aataaagaag aagctttttt
     3601 agatgagatt gaggcaattg aaatttcagc aattaaggaa ataggaaata ttatggcaag
     3661 ttcatatgta aatgctattg catctatgtt aaacatgact ataagtgttt caattccaga
     3721 tatatgtatt gatatggttg gagctgttct aaatgtgcct atgataagat tttcagatgt
     3781 tggtgacaaa gtcttattta tagaaaataa atttaaaatg agtgataatt attttactag
     3841 ccacattttg atgattccag aaatgagctc acttagagaa atactcgtga ggttgggatt
     3901 agaagtatga atagagagat agtagtgaac attgcagata tgaagatagc ctatagacca
     3961 aatgttttag ttacttatgc acttggctct tgtgtaggag tatgtttgat agataaagta
     4021 gctggaattg gtggaatgtt acacgttatg ttgccatata gtaaagatgc tattaacatt
     4081 gaaaataagt gtaaatttgc tgacacagga ataaatgagc tcattaaatc tatggagaat
     4141 ataggtgcaa atagaatata tataaaagca aaaattgctg gaggtgctca gatgttttta
     4201 ggaagcagca attcagtaat tgcaagtata ggacatagga atgttgaggc agtaaaaaaa
     4261 actcttttag gattaaatat tccaatatta gctgaggaca caggtatgaa ttatggtaga
     4321 acaatacgtt tttacactga gacgggtgaa ttacttgtag attcaataaa taaaggtaag
     4381 aagaaattat aatatatatt tcatagaaag tgtattttag gagggaggta ttaaatgaga
     4441 tttccagatg acccatattt agattatgga ctaattgatg aaggaattaa gtatctaaag
     4501 tttaaagtag atgagcaaga ttttggggtt gaacttgaaa aggtaataga aattactgga
     4561 aatcaagaaa ttatatttat tccagaacta cctacatatt ctaaaggcat aataaatcta
     4621 agaggaaaaa tcattccaat aatagatatg cgtttgagac ttaaaaaaga tgaattaatg
     4681 actacaaatt gcatgtatat agtagtaact gaaataaagg acttggtagt tggatttgtg
     4741 gttgatagag ttgatgatat agtaagctta gaaaaaagta aaatatcacc tccaccaaga
     4801 gttactatag aatattcgga ttattttgta agtggcgttg gagaattaga aggtaaaata
     4861 attactttac tagatttaga gaaacttttg acagataaag atgaagtttt aatagatgaa
     4921 ttaatcaatg ggtttgattg cactatcatg taacataggg atgtagttgg agtgttatta
     4981 taaaaagatt gtgatgagaa catttaagaa tatagtggta taattattaa gtataaaaat
     5041 tttaaatcat taaaatttga atatattatg tttaagagag gtttgtatat gaaaaataag
     5101 caacagggaa caattttagt cgttgatgat agtgttctga tgtgtgactt aatagataaa
     5161 acacttacaa aagataattt taaggttaaa aaagcattta atgcaataga ggcaaaagaa
     5221 tttattgaag aatttatacc agacattatt ttgcttgata taattttacc agataactct
     5281 ggttttgagt tttgtaagga agtaaaatct aatactcgta cagcagatat acctataatt
     5341 tttataactt caaaaaatac agatacagac atagtaaaag gatttggtgt aggagcaatt
     5401 gactatatgg tgaaaccttt tagtatgaca gaattaaaag ctcgtgttat agctcatctt
     5461 gaagtaaaga aatctcaaga taaattaaaa aagataaata atgaactgga atcttctctt
     5521 gaaaaattga atagactcgt tgtaagagat tatcttacag gtctttacaa taggcgacat
     5581 ataattaatc agcttatgga gcaaagaaga attaataaat atagtgaaac atcaatatcc
     5641 cttatccttg gtgatattga tgactttaag gttatcaatg atacttatgg gcatgaagca
     5701 ggggatttcg tgttaacagt aatatcaagt ataataaaaa agaattgtag acaaattgat
     5761 actgtatcaa gatggggtgg agaagagttt ttgataatgc tcacaaatac tccaatagaa
     5821 ggtgctaaga tactttcaga aagaatccgt aaagagataa aggagtttga tttcaactat
     5881 aaagaacata aaataaagtg cactattaca ttaggagtag tagaagtaaa ctcaaatata
     5941 tcattagaag aaaacatatc cttagcagat aaagcaatgt atgagggtaa aaacttagga
     6001 aaagattgta gcgtagtgag tacaatgaaa ggattaaaaa agatataaac ttactttatg
     6061 aaggagtaca gatatgaaaa gagaagtaaa gctttctaaa ttagcaattg tattgctgat
     6121 tattatagta ttggctgttc tagcagggat tttttcttct atttcatctt ataaagcatt
     6181 tgaaaaagta gaaaaaagta ttaatgatat gaataatttg aggactcaaa gtcagtatat
     6241 aaaagataca actagagaga taagtgatgt attaagaaga tatgtattta caggagaaaa
     6301 aaagaataaa gatatttatg aaaaagaatt atacggaaaa acaagagaat cagctttaaa
     6361 caaattagaa aaaattggtc ttacaaagag cgagtcaaat aaaatcttag aagcaaaaga
     6421 aatatcagat tctttagtac caacagaaca aagtgcaata aaggcagtcg aaagtaatga
     6481 taagagtaaa gctcaaagta taatttttgg tagtgactat ataaactccc aagagaaaat
     6541 ggattcttta ataaatgaat ttcaagagga tatagttacc cgttcaaatt ccaaaattgt
     6601 agaagtaaaa aaagaattac aaagtctaat attaagaaca atatttatac tttccctttt
     6661 aataatattg acaataatag aatatacagt aataaaatta aagatagtga ttcctattaa
     6721 tagagtagaa aaacactttt caagaattgc atctggggac ttaagaacaa gtatagacgt
     6781 tgaagaaagt aaatctgaaa taggactatt agtgagttct gtcaaaaaga tgcaaagtat
     6841 gcttttaaat tacatagacc ttatagacag tacattaact agtattgcaa aaggagattt
     6901 aagagtagag atagaccaag aatttatagg tgattttaaa tcaattaaaa tatctttaga
     6961 aagtataata aactctttta atggtgattt ttctgaaata aatttatcag cagaacaagt
     7021 tgcaagtgct tcagaacaag ttgcagcagg tgcacaagct ctttcacagg gtgctactga
     7081 acaagctagt tcagtagaag aattagcttc cacgattaat gagatatcag atggaattaa
     7141 gaagtgtgca gatagtgcta gtcttgcatg tgaggtatct gaccaatctg catcacaagt
     7201 tggttttgga agtagatgta tggatgatat gatgacatca atgggtgaaa taagtgaaaa
     7261 atcgagtgaa atatcaaaaa taatcaaaac aatagatgag atagcattcc aaactaatat
     7321 tctcgcatta aatgcagctg ttgaagcagc aagagcaggt caatatggta gaggatttgc
     7381 agttgtagca gatgaagtta ggtctttggc tggtaaaagt gcaaaagcag ctcaaaatac
     7441 agctattctc atagaagaag ctattaaaac agtagaaaat ggtgtcagaa ttgctaaaga
     7501 gactgcaaat gcattggact tggtagtaaa tggtgtagaa aaaactacta attatattaa
     7561 tggtatttct tcggctgtac atagtcaaga agtagcaatt acacagattt tatctggaat
     7621 agagcaaatt tccatagtag tccaaatgaa ctcagcaaca gcagaggaaa gtgcagcagc
     7681 ctcagaagag atgtctgctc aagctcaaat tttaaaagaa ttagtagaaa aatttcatct
     7741 aaaggaaagt gaagtaagag aatcatcatg ggtttaaatt atgaggtgaa tattgaagta
     7801 agagaattat catgtattta aattatgagg tgaatattga agtaagagaa ttatcatgta
     7861 tttaaattat gagatgtata tatattgaaa taagagaatg attgtgtatt taaattatga
     7921 ggtacatata gtgagataag agaatcatca aggatttaaa ttatgagatg catatataat
     7981 agaaaattat ttgtaggtcg taggtgataa atgtgaatta tattgaaccg agtatgagac
     8041 aaatgttaga tatgtttata tttgagacaa gtttattact cgaacaatta gatgaaatta
     8101 tgattcaaat agaaaaagag aaagtattta ctcataacaa cattaatgaa acttttagaa
     8161 taatgcatac gataaaaggt tcagcatcta tgatggggtt agacaatata tctgctttag
     8221 cacatagtgt agaagacttg ttttatgcta tacgtgaagg taaaatagaa gttttagaca
     8281 atgacctatt atttgattta gtctttcaat cattagattt tataagaaga gatatagatg
     8341 agctacaatc aagaggttgt atatcttctg attactctaa ttttattgaa ggaattgaag
     8401 agtatgtgag tactttggaa ttatcaagtt ctatgaaatc aaaggataat gcaaatttag
     8461 aaagtacaga cgaaaaagaa gcaatcgatt caaattttga atacaaagaa gactctgatg
     8521 tagtaaaaat aatctttgaa caaggttgca tgatggaaaa tgtgagagcg ttcatgcttg
     8581 tatttgaact tagagattac tgtgaattta tagattttta tccaaaagat gtagaaacaa
     8641 atgcagagtc tacagaatat attaaaagtt atgggtttta tatacaattt gtagctaaag
     8701 aggataaggc aatagtttat aaaactatag aagataatct ctatgtaaaa gaatacataa
     8761 atattagtaa aaaagaaata gaaatgaaaa atacatcaaa taatgatgca gtagaaaata
     8821 aattaaaaga taataataaa gtaagtaaaa gttatcaaaa taactataca gaagatgaga
     8881 tacaagaaga tgttttaaat gaaaaaactg atatggaaaa agatagtcta agtgaagaaa
     8941 atgaacattt accaataagt aagcagagta ttatgagtgt aaatttagaa aaattggatt
     9001 tacttcaaaa tatagttgga gaaattgtaa ttatggaatc aatggttgta aatagcgtaa
     9061 attctacagg tgttgatata gataatttca ataaggcaac aagacaatta cacaagctta
     9121 cagatgaatt gcaagatata gtaatgtcta tgcgtatgat tcaaattgct ggtgtatttc
     9181 aaaaaatgca tagaattgta cgagatatga ataaaaaatt agataaaaat gtagagttaa
     9241 taactatagg agcagagaca gaggtagata agaatgtaat tgataattta gcagaacctt
     9301 ttatgcacct tataagaaat gctatggacc atgcaataga gcctattgag gagcgtatag
     9361 cgaaaggtaa agaagaagtg ggaaagatag ttttatctgc tgagaatcta ggcggagaag
     9421 ctgtgataag tatttcagat gatggtagag gcattatgag agataagatt ttggcaaaag
     9481 ctaaggaaca aggtcttctt aaacatcaaa ttaatgagta tactgatgac gaggtcaatt
     9541 ctttgattat gactccaggt ttttctacaa aagaatctat aactgaattt tctggaagag
     9601 gagtaggcat ggacattgtt cgtaaaagta ttgaaaaagt tggaggttca attgaagtaa
     9661 aaagtaaaca ggatgaaggg acaaccttta ctataaaaat accattaact ctttctatag
     9721 tagagggtat gaagtttaaa gttggagatt cactatttac tttaccaata tcatccataa
     9781 gaagaacatt taaacttaca gacttaaatc aaataattac agaaggaaat aagacagagt
     9841 taattacaat atatggagca tgtattccca ttataaggtt acataaaata tataatattc
     9901 aaacaaaagt aaccaatctg atgcatggta atatgataat tattgaaaat gagcataaag
     9961 cagtatgcat ttttgttgat gaaataatag gtaatcagca agttgttgtg aaaccatttc
    10021 cagtttattt caaccgattt aatataaaaa ataagggttt atcgggatgt acaatacttg
    10081 gggatggaag tataagttta attatagatg cagatagcat tatagaaata tattagggga
    10141 tttaaagtat ggctaaagat gaaattatta aacaagtatt aacattttat gtaaatgatg
    10201 ttatttatgg aatagaatta gaaaatgtga ttgaaaccat aagatttcaa tcaataacgt
    10261 atgtaccatg tcttccaaca tatataagtg gagttattaa tttgagagga agaataatac
    10321 cagttattaa tatgcatata aagtacaatc ttcctaaagc tgattataat gagagaactt
    10381 gtataataat tacaaaggtt gatgagtatc aagttggaat tattgtagat aaagttgttg
    10441 atgttattca tatagaaaat ctaaatttac tagagacgac taattctaac aacactaata
    10501 ttaataaaga tataaaagag attgcgaaag ttgaagataa ttctatatta attttagata
    10561 ttcgaaagtt tttgattaat agcatatagt tagttctgat aaattagcag gaggaagttt
    10621 ttgcatgata aaactcacag atgaagaatt tatagttctt gtaaaccatg taaaaaaaga
    10681 atatggtatt gatttaagta aaaaaagagc gttaattgaa ggtcgcttat acaatacaat
    10741 gatagagaaa aaacttagtt cttttagtca atatatgaat ctattattta aagataagac
    10801 aggaaatgaa gctataaacc taataaatag attgagtact aaccacacat tttttatgag
    10861 agaacctcaa cattttgagt ttatacagaa tagtatactt cctttttggg aagaaaataa
    10921 taaaataaga aacataaata tatggagtgc aggatgttct agtggagaag aagcgtattc
    10981 aatagctatg actttggatg attattttgg atataataaa gagttgtgga acattaaaat
    11041 catagccaca gatatttcaa ctaactcact tgaaaaagct aaaagaggta tatacataga
    11101 atcaaatgtt aacaatgttc ctgagttatg gaagaagaag tattttatag acaacaagga
    11161 tggtacattt aaaatttgtg ataagattag aaaaagtgta atttttaagt catttaattt
    11221 aatgaatgat ttctcatatc aacattttga tttgattttt tgtagaaatg ttatgattta
    11281 ttttgattta aaaacaagag aagaacttat aaataaattt tataatgcta ccaaaaaaga
    11341 tagttttttg tttgtaggac atgcagaagt aatcaacaga agtagtacta aatacaaata
    11401 tgtaaaacct gctatatata aacgataaaa aggtattttt gatatatgat tttttggagg
    11461 cggtaatgtg gaacgtttta ataatatggt tattgctata ggcgcatctg taggtggtac
    11521 agaagcaata cttgaaatta taaaagacct accaaaatca acaccaggga ttgttgtagt
    11581 tcaacatatg ccagctatat ttacttatat gtatgcacaa cggcttgaca agcagtgtat
    11641 tatgaatgta agagaagcta agaataatga tagagtcgaa caaggaaatg tgttaattgc
    11701 acctgggggt taccagatga aattgtgtac ggataaacaa ggatattatg taacttgtga
    11761 aaaaggagaa agaataagtg gtcactgtcc atcagtagac gttttgtttg attcagtagc
    11821 agaagtagca ggtaaaaatt caataggaat aatacttaca ggtatgggtt atgatggagc
    11881 aaatggactt ttaaaaatga aagaaaatgg tttatttaca attggacagg atgaaaattc
    11941 ttgtatagta tatggaatgc caatggtagc ttttgataga ggttctgtta tgttacaatt
    12001 acctcttggt gaaatttcaa actgtttgat aagacatatt aatattttaa aacaaaaaga
    12061 ttaatttttt ataaataaaa attaatcttt tattaaatta tatggataaa aaaccctaga
    12121 aactccaaat ataataagag agatttatat ttaggaggaa aatgataata tgaaattttt
    12181 taaagtcgat acaaaagcta aagttttaag atatgtattt gttttaacat tgactataat
    12241 ggctgttttg gcaattaaac atgactataa atataataat ttaaggaaag atgcttcaat
    12301 taatttaaat agtaataagg aagcagtgac tgttatgagt aaccagtcat taccaaaggg
    12361 taatacaaat agtaaacaaa ctttatcagt aagtacaata ggaagtaaaa caatagtgaa
    12421 aaatgatgag tatttagaaa gtactataaa catgcctgta ataacaaatt caaataaaat
    12481 tgttgaaagg tctacaaatg ataagataaa aaatgacata tttgaatttt ataataaatc
    12541 atataaagaa gcaaagcagt atcttaagga taatcctgac gagaaaaata agtttgttgc
    12601 aaatgtagac tttgaactta aaaagaatac tgattctgct cttagtataa aagtgagata
    12661 ttatacttat agtggtggag cacatggatt ttatcaagat atagcatata atgtggatat
    12721 gagaactgga aaatttttag aattaatgga cttatttaag gataatacta aatataaaga
    12781 agttatagat gaagaaatta aaagacaaat agctgaactt gaaaaaaagg atgaagaaaa
    12841 tatcggaata tataatttta aaggtataaa agagaatcaa aacttttact tgcaagatga
    12901 gaatctagtt gtatattttg atttatatga tataactcca tatgcagcgg gaataccaga
    12961 attttcaata aataaagcgt tgataagttc tatgctaaaa ccagagtatg tagatttatt
    13021 tgatttgaaa tagaagatta aattaaatgt tatgctataa tatttagcat agaaacttat
    13081 taaaattggg ggtataaaaa tgctactttt aaaaaaagac gacataaaaa aagtatttac
    13141 aatgagagat gcaattgaag ctgataagga agcttttaga atttattgtg aaggaaagag
    13201 tgtaaatcct ttgagaacca atatatctgt tccaagtcaa gatgctagta tgttatttat
    13261 gccaggatac gtagaagaat taggctgtgg gggaataaaa attgtatctg tatttcctaa
    13321 aaatgctcaa aaaggcaaac cagtaattcc agcaacagta ctattattag atggagaaac
    13381 tggagaagtt agtgcagtat tggatggtac ttatgtaact cagattcgta ctggagcagc
    13441 ctctggagca gctatagatg tattggctaa aaaagattct aagattggag ctttgatagg
    13501 tctaggagga caaggtgagc cacaacttga agccatgata gaagctagaa atttggatga
    13561 ggtaagagtt tttagtagaa acagggaaag tagagaaaaa tttgcagaag aaatgaactt
    13621 aaaattaagt aaatataata ctaaaatagt tgcagttgaa tcatctgatg aagccataga
    13681 taacgcagat gtgatagttt tagctactcc atcaaaacaa ccagtactta atggaaactt
    13741 agtgaaaaaa ggagctttaa taagtgcagt aggttcatat atgccagata tgcaagagtt
    13801 gtgcccaaac tgtttaacta gagcgtctaa gatatttttt gaatcaacag atgcagtttt
    13861 atctgagtca ggagatatat taattccatt aaaagaaggt aagataagta aagatgattt
    13921 tagtggtgat ttaggaaacg ttataaatgg aactattcct ggaagagaag atgatgatga
    13981 gataattgta tttaaaactg ttgggatagg tgtacaagat gtcgtaacag caaaaagaat
    14041 atacgataaa gcaaaagaaa acggtattgg aatagactgg aagtggtaaa atattcaaaa
    14101 taattctata gttagacaaa tttatgttat aatgttgata aataaatttt ttattatttt
    14161 aattgaggga ggatgtttgt atgaaaatgg taaaaaagtt aatattgtct acgattatgg
    14221 ctctagtttt agttctaact atagggtgtt ctaatgcaga agaaaaagta aagtcgaatt
    14281 ttgaagctaa tttagctgca ttagaaatgt ataagtatga tgaaaaagga atttctgatg
    14341 cattaacaga agctgaatat aaacagattc aaacttataa cagcaaggac aaggtttcaa
    14401 tgattgctca attgacacct ctttatggta aagaaaatgc tgaaaagata tacgaaacaa
    14461 ttgttaaatt aattcaaggt gttgaaaaga cagtaacagt ggaatcttct agtaaggata
    14521 agataacttt gaaagttgta agtaaagctt tagatactag taaagtaatg agtgaagcga
    14581 acactttagt aaaatcttat gtaactgatg aaaagaatta cgataaaaaa gaaaaagaag
    14641 ttcaagatga tatgacaaaa atgattatag acctttacaa taagaaacca actaaggaag
    14701 ttacaactat gattacatat actaaaaaag atggtaaatg gcaaccacct agtaatatac
    14761 aaaagttatg tgaaggaata tatacaattt aatttatttt aaagacacaa aactatcttt
    14821 taagcatata ataaaatgtt tggaagatag tttattttat tttcaaagta taaattatat
    14881 tttataaatg atttatttta taaataattt atcttgataa atagtaaata atttacttta
    14941 ctaataataa ataatttatc ttac
//
LOCUS       DAEPOZ010000090        14781 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.090, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000090 DAEPOZ010000000
VERSION     DAEPOZ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14781
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.090"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            106..2004
                     /locus_tag="KR440_001971"
     CDS             106..2004
                     /locus_tag="KR440_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HBH0699022.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
     gene            complement(2132..3253)
                     /locus_tag="KR440_001972"
     CDS             complement(2132..3253)
                     /locus_tag="KR440_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HBH0699023.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLEEFKTVIHPEKIADYISELIDNHYS"
     gene            3452..3784
                     /locus_tag="KR440_001973"
     CDS             3452..3784
                     /locus_tag="KR440_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBH0699024.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            3771..4829
                     /locus_tag="KR440_001974"
     CDS             3771..4829
                     /locus_tag="KR440_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBH0699025.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNIKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSVTIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINEDSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            4822..5892
                     /locus_tag="KR440_001975"
     CDS             4822..5892
                     /locus_tag="KR440_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBH0699026.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAEFKK
                     ENKLYQEKVNIDKLDISVDNINVTIKKSSSEDVRVTTRGNNEFYKYNVTLKDKTLVVK
                     GERKYENKIKKIKNFDQLLNTSINSMFSHDYREIIIYVPNNVDINASSTSSYLFVYDN
                     VASNTITYKTSYGGFSRAITENKVNRLENLNLISNSNLHLSTKSILGVKNVNIESESL
                     DISSENEDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSNMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHSEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            5994..6530
                     /locus_tag="KR440_001976"
     CDS             5994..6530
                     /locus_tag="KR440_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBH0699027.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            6647..7354
                     /locus_tag="KR440_001977"
     CDS             6647..7354
                     /locus_tag="KR440_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBH0699028.1"
                     /translation="MNNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRKLIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVIGYQGEVSKEQN
                     LEKLFMKVVAENRKRGE"
     gene            7356..8072
                     /locus_tag="KR440_001978"
     CDS             7356..8072
                     /locus_tag="KR440_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBH0699029.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWAGKMIYCIICCSIASIIS
                     AIGVVFVGIIYKETISIGQNIIATILIILLFMWQIPLCMFLASKFGIFVTVLTNMFFT
                     IVGVAVFSTTEMWYVFPYSIPSRLMVVVLHILPNGLPVPEGSELLNNAVILPGVLISV
                     ILFVLFALVTRAWFNNREVK"
     gene            8074..8832
                     /locus_tag="KR440_001979"
     CDS             8074..8832
                     /locus_tag="KR440_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBH0699030.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSGVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFTSSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLMCGFVTILAFVILCIWFSKWEGKKSEE"
     gene            8976..10364
                     /locus_tag="KR440_001980"
     CDS             8976..10364
                     /locus_tag="KR440_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HBH0699031.1"
                     /translation="MLRSKKSNNKKVEKLRTIFIKYLSLFFVMTISFALLLMLSFSVL
                     LSSGVILPANYSEKQFDKYKEKIISSEKAIEDIIPNLYEYGVYTLDGNLISGTFNKKE
                     SKQVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKLVSEYSSPVLRAYLPKPEMLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNILF
                     SMDKMKLALKNSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAINMSEFIKDVLLQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVSNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGILYIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(10440..11261)
                     /locus_tag="KR440_001981"
     CDS             complement(10440..11261)
                     /locus_tag="KR440_001981"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HBH0699032.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYDRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            11497..12666
                     /locus_tag="KR440_001982"
     CDS             11497..12666
                     /locus_tag="KR440_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HBH0699033.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSEDIRNYIGQQDGV
                     ELQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            12849..13049
                     /locus_tag="KR440_001983"
     CDS             12849..13049
                     /locus_tag="KR440_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBH0699034.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            13470..13898
                     /locus_tag="KR440_001984"
     CDS             13470..13898
                     /locus_tag="KR440_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBH0699035.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(14041..>14781)
                     /locus_tag="KR440_001985"
     CDS             complement(14041..>14781)
                     /locus_tag="KR440_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBH0699036.1"
                     /translation="LATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLLNDLVN
                     QELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKFFEANK
                     SSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGGDLGTF
                     GRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAEVEKSL
                     LYQKQNEVYGNKINALNAKYGNLVKYND"
BASE COUNT         5788 a         1621 c         2427 g         4945 t
ORIGIN      
        1 aaacacgaat aatttgcaag aaaaattcat gaatgtaagg aaaatgaaat ttatcttgta
       61 tttaacaaaa taaaatatac ataaaaatta tgggagagtg gaaaaatgga tagtctatta
      121 tacgtaatag agaaagataa acacactaat gaagaattaa gagaaatcct aaaaagcaat
      181 aaaaatataa gatttgtttc cttaatgggg gttgatttag gaggaaatgc aacagatgaa
      241 aagattcctg tagaattatt tttggaggat atagataaat ttttggaatc agctatacag
      301 actgatggtt caagtgttga gctttataat atagctacat taaataatgc gaaagtagat
      361 ttaatgccag ataaaagttg tcattggtat gtagattaca atatggaata tatagacgaa
      421 gaagtaggtc tacctgttgg aacattaaag atacctgcat ttttaataca tgataataaa
      481 aaagtatgtt ctagaggagt acttcaaaaa gcagataagt actttaaaaa gtcaatgtat
      541 gaaatattta gagaatatcc acatgtaata aataacatag gaatagattc agtagatgat
      601 atagaggaaa taatgttaac agcagctaca gaacttgagt tctgggttaa tactcctgag
      661 gataaagctg acttagagaa attatatgta tcacaaagcc taaaagagca atactggaaa
      721 agaactcatg gtattataag aacttgttta gagaaaagtt taattatact tcaaaagcta
      781 ggtgtaaacc ctgaaatggc tcacaaagag gtcggaggaa tacaaagttc tattagtata
      841 gatggaagaa caaaccatgc tatggagcaa ttagaggttt cttggaagtt ttctacacca
      901 ttacaagcag cagacaatga attattagta agagatgtta tagaggatgt atttacatca
      961 catggactag aagttacatt taaagcaaag ccaattcatg gagtagcagg aagtggtgga
     1021 catacacatg taggagtaag tgcgaagtta aaggatggaa gtataaaaaa tctatttgct
     1081 ccaaaagatt taaaagaaga ttatctaagt gaactaggat atggggctct tatgggactt
     1141 ctttacaatt atgaagtatt aaacccaatt gtaacagctt ctaatgatgg atttaataga
     1201 ttagttcctg gatttgaagc tcctgtatgc atagttacat ctttagggca tagttatgag
     1261 attccatcaa gaaataggtc agtgcttgtt ggattaataa gagatatgaa aaatccaaag
     1321 actgtaagat ttgagttaag gtcaccaagc cctctatcta acacgtattt agtaatagca
     1381 ggatgttatc aaacaatgtt ggatggtata aaagcagcag ctaagagtgg tcttagtaca
     1441 aaagaacttg aaaaagagtt atctaaaaat gtaggtgaag aaagtttcta tttagaaaaa
     1501 gatagagcat atagagatga aaatgatgta tttgaacatt atagtttgga agaaagaaat
     1561 gcaagatttg gaataccacc ggctacagtt tatgaaaata tgaaaaactt agaaatatat
     1621 gcttcaaaat taaaatcttt aaaacaaggt gatgtattta ctgatagtat aatagaatca
     1681 tttaagattg gagctataga taagtggcaa aaaaaattaa aaactagaat aatagaggct
     1741 gggattcaaa aaataagaag tattgtcaag atacacacaa aagaaaatat ggatgcgctt
     1801 gatgaggtag tttggaactc aatatcggat ttaaagttca atattatgaa ggatacttta
     1861 actagagaat cactattcac tagagtgaga gaagctatag aaaataagga ttaccagact
     1921 gcttcagatt tacaaataga attaaagaga agtatggaag aaatacaaca actgtatatg
     1981 cagtacaaga aaaatatata ctaatataac aatttgattt tgatatgata aaggcaatca
     2041 agtattatta aacactatga gtaaattctc aagtgtaaaa gcatacttga ttgcctttaa
     2101 ttttatgttt gaaatttttt aatagtacaa tttaactgta atgattatct attaattctg
     2161 atatataatc agcaattttc tctggatgaa taactgtttt aaattcttct aaattttttt
     2221 tcataaagtt tattatagaa tcaaattcat ttgatactaa tttataaaat atgtttttaa
     2281 ggtctaggtc atcactataa atcatgccaa gacccttatc tattataaat ttagcgtttt
     2341 caatttcttg tccaactttt ggcattttca cgagtactgg agtttgtgat tgaatagctt
     2401 caaatatagt tgctcctcca ggcttagtta gcattaggtc acattctctt attagattat
     2461 acatgtcgtt tacaaatcca agtactttaa tattttttag aggtttttta tgagttagat
     2521 tgtcatataa ttttttgttt ttaccagtaa ctattgtaat ttctatagaa ttactatgtt
     2581 cttcaataaa ttcatctatc caatgcataa aatcctcatc tatatcaaat aggcctcttc
     2641 ctccacctag aagtaatagt ctatatttat cacaacatag ttctttactt tcaatttgaa
     2701 atctcttatc aacaggaact ccagtaacct ttatggagtc tggatttatt cctttctgaa
     2761 aaaatctatt ttttatttca ggagaaggaa caaaatacaa atctgtattt tcaaaaaccc
     2821 attccatact atctacaaca tctgttataa ctgtaagtgt aggtatattt atatcaggat
     2881 tttttatctt aaagttattt acacaagcag ccgctagtgg aaatgtagag ataatcaaat
     2941 ctggatttat atcagcaata tactcagaaa gtttaggtgt atataattta tgagataaat
     3001 catgtcttga atcaaacgat ttcttaaagt aataatagta attatataat ccaggagtat
     3061 attttgtatt gttttcataa agttttacca taggtttatt catcatggga atacttgcat
     3121 ttataaagtt ttgtataaca acattatagt tactatattt ttcaataata cattctttga
     3181 ctgcttttgc agcacttata tgtccagcac caaattgagc agttaagatt aatattgttt
     3241 tatcatcttt cattttagcc aatttatcac cactttcata aaatatatct gattagagta
     3301 ttatacattt atagaaggat ttaatcaaca agaatattta aaaataacta ttgtgcagaa
     3361 catagtactg tgttattatg aggatataag aagttttaac atagtacctt gtaaaacaca
     3421 atactgtgta tttaaagaaa ggatggagta tatgaacaca caatttagaa agggtgtttt
     3481 agaaatttgt gtacttgctc tcatatctaa gaaagatatg tatggatatg agatagtaca
     3541 taacatctct aaagtaatag atgtaaatga aggaacaata tatcctattc taagaagact
     3601 tacaaaagat ttgtatttcg aaacatatat actagaatct aatgaaggtc ctgctagaaa
     3661 atactataga ataacttcac taggaaaaga gaatttatcc aatctgatgg aagagtggag
     3721 agaatttgta aaggctgttg agattttaat aagtgaagat gagggaggta atgaacttga
     3781 ataaaactga gtttcttgaa attttaaagg attatctaaa aaaagatttt tctgagagcg
     3841 aagttaatga tatattgaga gattatgaag agtatttcgt agatggtctt atagagggaa
     3901 agagtgatat ggagataata gcatcacttg gttcaccaaa atctatagcc aaagaactta
     3961 tcttgcagac taaagaaaaa aatattgaag aggaaaaaat aataaataca gatgttttga
     4021 atgataagtt tagtaaattt aaatcagata tgaaaaaagg ttactctatc ttaaaagatg
     4081 aagcaaaaga aaagtatagt aaatcaaaag aaaatataga tggaaaaata atgaatacag
     4141 atatattaaa tggtaaattt agtaggttta aatcaaatat aaaaaaggat tactctatct
     4201 tgaaagatga agctaaggaa aagtacaata aatcaaaaga aaatataaat gataaactta
     4261 caccaagtat agaagaaggt gtagggggat tatctagaca agttataaag ggattattag
     4321 tagtgttatc tttatttcta attgttccag catttgcgtt tgtatgtgct attgtatctg
     4381 ttactattgc attagtatgt tcattaattg cttttgttat attaattcca tttgtgatta
     4441 aatttatagc aatggttcca gatgttatga tgttttgtat tttcgcaacc atagcatttg
     4501 tagggcttga gatacttgca tggcaaattt tattatttgc tataaaacta ggtaaacaat
     4561 tactaaataa ttatatacat tggataaaga gaaaaaatat ttatataaag gcaaatgaaa
     4621 agatggaaaa aagaaaaaaa gctactttaa atgatgaaga tttgaataaa gacattaatg
     4681 aagattcata taataatgat tactacaata gagaagaaga aagcataaaa gaaactgatg
     4741 aatataatga tgatagtata aattttgagg cgatatataa ggaccttgaa gatatagaaa
     4801 ataagaaagg agaagataaa catgaataaa aaacttgcta tatttgctgc agttttattg
     4861 gtaataggta taatagggac aacatggtca gggattttag ttatgccaag cctaataaac
     4921 tttggtttag aaaaggaagc tgaatttaaa aaagaaaata aactttatca agaaaaagta
     4981 aatattgaca agttagatat atctgttgac aatataaacg ttacaataaa gaaaagttca
     5041 agtgaagatg tgcgtgtaac aacaagagga aataatgagt tttacaaata caatgtaact
     5101 ttaaaagata agactttagt tgtaaaaggt gaaagaaaat atgaaaataa aattaaaaag
     5161 attaaaaatt tcgaccaatt attaaataca tctataaata gtatgttttc acatgattat
     5221 agagaaatta taatatatgt accaaataat gtagacataa atgcatcaag tacaagttca
     5281 tacttatttg tatatgataa tgtagcttca aatactataa catataaaac ttcttatgga
     5341 ggttttagta gagctataac agagaacaaa gtaaatagat tagaaaacct aaacttaata
     5401 tctaatagca atcttcactt gtcaacaaaa tcaattttag gcgttaaaaa tgtaaacata
     5461 gaatcagagt cattggatat atcttcagag aatgaagatg tatttatcaa taatatagaa
     5521 gagtatatac ctgaaaatgt aaatataaaa gagaaaatag gtagaaattc aaattatgaa
     5581 agtgaatttt acttgtactc taatatgcca attgctaagt tcctggacat agaagttcca
     5641 aattctaagg taagacttga tataccagta aataagtata aatttaattg tgatataaag
     5701 tcaaaagaag caattgaaga atttaataat gatgaagaat atgacaatga ttcatatgat
     5761 tatgaacata gtgaagaaca tagcaacaag tatagaaata cgagggaaat aaaagggctt
     5821 ttgaataaaa atctgtcgaa tttagagaaa gaatatacta taaaaataaa ttcaaatagc
     5881 ttagaattgt aaataattta ctataataat ttataaattg atttaaaagt ataaaaatat
     5941 aatctatact atggtataat aagtatcgaa agagaattag gagagggata cttatgaata
     6001 aaatagcagt aagtttttta ataattgcta ctactttatt gagtacagca tgtatggatt
     6061 atagcatcag tgcagttgag ctagttgatt ctaaagaatc agcagttgta aagaaggatg
     6121 aagatgcaaa agaagaaaca actagtaaaa tgattaattc taaaaaaact actaaaatac
     6181 ctatagagat aatttcaaag gatgagaaaa tagtaaagta cttacagatt gatgaagaaa
     6241 gttcacttaa ggataaactg cgattaatct tagatacatt atcaaatgag tattttaatg
     6301 gtttagacat ggaagtagaa gttaaggaaa aagataatct tgttaaaata aacttgatag
     6361 aaccagataa aaaaagtaga gtttcttgga aagatgatta tttgaatgaa caaaatataa
     6421 tatatactat aaataatatt attaaaaatg tcatccaaga agaagataat agcatttgga
     6481 ttgaagaagt agagatatac tacaatggaa aattaataga attaagataa aaaaagatgt
     6541 gtttttaaca catctttttt ttgtttttta attcttataa aatccttata tttatgattt
     6601 agaatgattc ttgtaaataa gtttgacaaa agaaaggatg aaaattatga ataattatat
     6661 tttgcaggta aaaggagttt ctaaaaaatt taaggagcag atggcagttg ataatatttc
     6721 actagcaatt aaatctaatt ctatatatgg attattagga ccaaatggag ctgggaaatc
     6781 tactttatta aaaatgataa ctgggatttt aaatccaact tcaggaaaaa taatgtttga
     6841 gggacataga tggactagaa aggatttatc aaatattggt tctcttattg agtctcctgc
     6901 tatatatgag aatttaacag cacgagaaaa tttaaaagtc catactactt tacttggtct
     6961 tcctgactct cgaattgaag aagttttaga gactgtcgac ttaagagata cttttaaaaa
     7021 gcgttcaggt caattctctc taggtatgaa gcaacgtctt ggtattgcta ttgcattatt
     7081 aaatcaccca aaacttttaa tcttagacga gcctacaaat ggtcttgacc ctattggtat
     7141 tgaagaactt agaaaattga ttcgttcttt tccaaaacaa ggaattacag ttattttatc
     7201 aagccatatc ttgtctgaag ttgaacaagt cgtagatgaa atcggaataa tttctaatgg
     7261 agtaattggt tatcaaggag aagtatcaaa agaacaaaac ttagaaaaac tttttatgaa
     7321 agttgtagct gaaaatagaa agagagggga gtaatatgga aagatttatt agagtggaat
     7381 ttcaaaagat gaaaggaact tttggaatga agttgatatg gatagttcca gtgttttgta
     7441 tacttttgtg tggactaggt ggagggcaaa atgctgcttt caactggtgg tatacgacat
     7501 ttcttccagg agcattaaca ttaatatctt ctttaatagt attaaaagat gaaaaaagta
     7561 aatatcgtgg tattttacca ttgccaattg aaatgaaaga cgtttgggct ggaaaaatga
     7621 tttactgtat tatttgttgt tctatagcat cgattatttc tgcaataggt gtagtttttg
     7681 ttgggataat ctataaagaa actatttcaa taggtcaaaa tatcattgca actatattaa
     7741 ttattttact atttatgtgg cagattcctc tttgtatgtt tttagcttca aaatttggta
     7801 tatttgttac agttctaaca aatatgttct ttactattgt tggagttgct gttttctcaa
     7861 ctacagaaat gtggtatgta tttccatatt cgattccttc aagattaatg gttgttgtat
     7921 tgcatattct accaaatggg cttcctgtac cagaggggag tgaactttta aataatgctg
     7981 ttattttacc aggagtatta atatctgtta ttttatttgt attatttgct ttagttacaa
     8041 gagcttggtt caataataga gaggtgaagt aatatgagac aactaataag aatattttgt
     8101 tctgatttaa taaaattaaa gagaaatttt ataatactaa tgcacatttg cattgcttta
     8161 atggggatgg gattgttttt atggtattat aaggtttcag gagctgataa tatattaaag
     8221 atagtagcat atttacaagt tatagcaata gcttttcctt tattgtcagg tgttgtgtgt
     8281 tcaatgtttg ttgaacaaga atattatgca gggaattata aacatatgtt aacaacatct
     8341 aatccaaaat atcttactct tataagtaaa tatataatct tagtatgtct tggttttgta
     8401 gcaacattag tgtctgtatt aggttttaaa tttggtatat ctaaggctta ctttacatca
     8461 agcttttata tgatttcaat tctaatttta attgggtgta atttatttga atacatatta
     8521 catttattct taagtttaag gttcggaaaa ggaacatcaa ttggagtagg tatagttgaa
     8581 atgttacttt cagcattatt attaactgga ttgggttctg gcatttggca gtattttcct
     8641 tgtgcatggg gtgttagatt tataactatt tggtcgtctt tttcaagttc taaaacggtt
     8701 gaatatataa aggttgaatc aataaaagca tatcaatcaa caggtttaat gtgtggtttt
     8761 gtaacaattt tagcatttgt aattttatgt atttggtttt ctaaatggga aggtaaaaag
     8821 tcagaagaat aaataaaaat tgactgttta tttacattat atttatatta ttatgttaaa
     8881 atataactta gcaatgaaaa atataaaata atcaaataaa aactggttag gtattaatta
     8941 tgaattaatt aaaatatttg gaggatatgg cacaaatgct tagaagtaaa aaatctaaca
     9001 acaaaaaagt tgaaaagtta agaactattt ttataaaata tttatcttta ttctttgtta
     9061 tgacaatttc atttgcactt cttttaatgt tatcattttc tgtattatta tcatcaggtg
     9121 taatattacc agctaattat tcagaaaaac aatttgataa atataaagaa aagattattt
     9181 caagtgaaaa agcaattgaa gatataattc caaatttata cgagtatggt gtttatactt
     9241 tagatgggaa tttgatctca ggcactttta ataaaaaaga atcaaaacaa gtatggaatt
     9301 taatgagaga tattgaagaa aggcatgcat attcagaaag ttatataaag ttttttaaga
     9361 aagatgaagt ttttataata aaatacaaac tagtatcaga gtatagttct ccagttttaa
     9421 gagcatattt accaaaacca gagatgttag gtatgattat ttttagtata atatttttta
     9481 ttgaaattgt tatattatca aaagtatttg gcaagaaatt taatattgaa atggagcttt
     9541 taaaaaatac tacagaaaaa atagaacaac aggatttaga ttttgttgta gaatctagta
     9601 aaatttatga aattaacaat atcctttttt ctatggataa aatgaagttg gcgttaaaga
     9661 attctttaga aaaacaatgg atgctagaag aaaatagaaa ggaacagata tctgcactgg
     9721 cacatgatat aaaaacacca ttaacaatta tacatggaaa tactgactta ttaattgaaa
     9781 taaatgaaaa tcctgagtta ttagaataca tggaatatat ctcaaagggt gtcactcaaa
     9841 tagaaaaata tattaatact ttaatagata tatcaaaaac agagaatggc tatatattaa
     9901 ataaagaagc aattaatatg agtgagttta taaaagatgt cttactgcaa atagaagcat
     9961 tagctagtac taaaaactta gatgtggagt tttataaaca agataatttg cctaaaaata
    10021 taactataga tagagaactg ttattcagag ctattatgaa tataatttca aatgctatag
    10081 attattcacc aagccagagt aaactataca taagtgtatc agtaagtaat aaatacttaa
    10141 aatttgtagt tacagattgt ggaagtggtt tttctaaagc agatttgtca aaagctacag
    10201 aacagtttta tacaggagat ttgagtagaa attctaaatc tcattacgga atgggacttt
    10261 atattgtaaa taatattgta caaaaacata atggaatttt atatattgaa aattctgtga
    10321 aaactggtgg tgctatggta acaattgaaa taccaatagt ttagtaaata atatccattt
    10381 tgtaaatacc taaattttat gatttatagg tatttataaa atggatagtt tttttgattt
    10441 tatatattag ttaaatctga taaaaagttt tcaaacataa ctccatcatt tctatcagta
    10501 tcaaattgag atgtgtaatt tatagtttta tatataaaat ctgttgcagt attaaccgct
    10561 actcccaaat catgtttatt tactaacata ccacaaagta tagatgtgaa tatatctcca
    10621 gttccactgt aagaacaatt attgtatttg actgatgtaa agaatacatg gtcattatct
    10681 ctatcataag ccaaatttaa tatattatca tcttctaata taccagtaat aacgactttg
    10741 ctaggtccca aatcagatac agattttgca atatatataa gctcatctct attataatcg
    10801 cttttagtat aatcatttcc agttaagaaa caagcttctg ttaaattagg agtagttaaa
    10861 tctgaatgtt taactaattc tttaatttcc tgtctcattt cttctgtgaa tattggatac
    10921 ataactccat tatcacccat cacagggtca actacgataa aagcattggg ataatcattt
    10981 ataaaattag ccactatctc aatttgatgc tttgaaccca aaaaaccact atatatacta
    11041 tcaaaattga cttttaaatt tttccaaaca tttgaatatt ttaccatttc atcagtaaag
    11101 tctaagaaag taaattcagg ataaccagtt tgacttgaaa gtatggccgt tggaaatgga
    11161 caacattgaa cctttaatgc tgaaagtata ggtatagcaa ccgataatga gcattttcct
    11221 attccagata agtcattaat tgctgcgact tttttttgca taaaaaaccc tccattagta
    11281 tgtattaatt ataatgatac caaaatattt atatacatat atattactat aaaaaaaaga
    11341 aaaaagtaga ttataaaata aaaaattgta aaaaaaacag tgtattgata caattaattt
    11401 ttaatattgg acataataaa atagagaatt attaaatatg aaaattattg tatatgacag
    11461 aatcttcttt gtaatttcat ttataaggtg tactatttgg gaggtgtagc tttgaaaata
    11521 ctgaaaaata agaataagta catcaaaggt ataatggcat ttgtattttt gtctttagct
    11581 actacagcag gtgtattttt aatcaatgat ttaagtatga aaaatatgga agaaaaagtc
    11641 atggaatctg caaatgttga aggtgatgat gtaaaagacc aatataaact attgcttgaa
    11701 aacttatttg actacagaaa taaggcaata ttagagaaaa atgaaaacat tttaaaaggt
    11761 ctttatgaga cggataaaaa atttggtctg tgggcttatg aaaatgaagt aaaaaagatg
    11821 aagtatttag aaaactggtc tagtaagcaa ggtgtaaaat ttaaagacat aaagacaagg
    11881 gttaagatta aaaaaataaa agaaaaagaa aaagacctct atggtataat ctgttcagta
    11941 tcgactgaat ataagtattc atatgaaaat caaaaggaca aagaaaatgc ctttagaata
    12001 ggtacatatc attatctaaa tgtaaaaatt atagataatc aatatgtcat aactaaagag
    12061 tggtacacag acccttttgc agactcttta aatttagaaa atataaaatc tgaggatata
    12121 agaaactata ttgggcaaca agatggtgta gagttacagc ttacagatga acaaaaaaag
    12181 gctatagatt atgcacataa atactgtgga gccgcagctg atgaagaaca tggtatgaaa
    12241 tttaatccaa actataggga ttataatcca gaaggtggag attgtgcaaa ctttgcatca
    12301 cagataatgt ttgaaagtgg aaggttcaaa aagaactcaa tttggaatta tagcgagaga
    12361 gcaggtacaa aagcatgggt aaatgcacaa gcttttaaaa attatgcact gtatagtgga
    12421 agaggttcat taattgctaa aggttcctat gaagaagtat acaaagaagc ttataattta
    12481 cgtccggggg attttgttgg ttatgagaag aaaggcagaa taacacatgt ttcgacagtg
    12541 acaggattag actcaagagg atatcctttg gtaacttgcc ataatactga tagaatgttg
    12601 gtgccatggg atttaggctg gagtgataag tccataagat tccatataat aaaaataaat
    12661 tattaaaaaa ttataaagaa aaataaagtg taaaagataa taatcattta aaattataaa
    12721 gtaaaaaaat tgattattaa tgtataaaaa taataataat tgacaaacat agctatatat
    12781 gatagagtag taataaggtt acagaaaaga gtagctaaat atttgtgttt ttttaggagg
    12841 atttattaat gaaaactggt gtagttaaat ggtttaacaa cgaaaaagga tttggattca
    12901 tatctgtaga aggtgaagga gatgttttcg tacatttctc agctataact ggagatggat
    12961 acaaaacatt agaagaagga caaagcgttg agttcgaagt agtagatgga gctaaaggtc
    13021 ctcaagctgc taacgtagtt agattataat taaaaattca tatatattag tgttctagta
    13081 atatgagaga taagccgcta atcatttagc ggttttttca gtttttgtaa ataaaattaa
    13141 tagcaaaata aattaaaatt attaaaataa aatcattaat aatgagataa atatgttaaa
    13201 attaatagtg tagagtaaaa ttattatggc taagtttaag tcaattaatt tttagtaaat
    13261 tttctaaaaa gagaataagt gatactgata gaccatataa aaatattaat aaagcataat
    13321 aagattaaaa tgagcaaagg aaaaacgctt gttacagcgt atagtattaa gaatacttag
    13381 aaaataagaa aggagaaagt tactgcaaaa aagtaacatt ataaatattg agtaatattt
    13441 ataagttttc agtaacggtg ggtagtgtaa tgaacatatt attctttcta acaccaaaaa
    13501 gtgaagtagc atatatatat gaagactaca ctataaggca agcactagaa aaaatggaat
    13561 atcacaaata ctctgcaatt ccaataataa gtaaggatgg taaatatgtt ggaactataa
    13621 cagaaggtga ttttttatgg acattaaaaa acgatttaaa tctggactta aagggattgg
    13681 aagatgttcc agtgacagat ataaatagaa aaatggataa ttctcctgtt tccatcaacg
    13741 cagatattga agatttagtt ataaaatctt taaatcaaaa tttcattcct gttatagatg
    13801 accaagacac atttattggg ataataaaaa gaagagatgt aataggttac tgttatgaga
    13861 taatacgtgg atataaaaat ttagctaatg ataattaaaa atcttattta aacttttaag
    13921 ctgagttctt tacaataaaa gaatgataat atgaaactga atatttaaac tttaatataa
    13981 aaagctgtct tttctaataa atattgtgaa aagacagctt ttttatatat gaccttatga
    14041 ttaatcgttg tattttacaa ggtttccgta cttagcatta agagcattta tcttatttcc
    14101 ataaacttca ttttgttttt gatataataa acttttttca acttcagcct taacttcatc
    14161 aaatgtagac tctgtacttt cttgaaggtc ttctaatttt attatatggt atccaaattg
    14221 agttttaact ggttcactta cttcaccttt agccatagag aacacagctt cttcaaattc
    14281 aggaaccatt tgtcctctac cgaatgttcc taaatcacca cccatatctt tagatgggca
    14341 agaagaatgt ttaagagctg catcttcaaa agatatttct tctgatttta tttgagcaag
    14401 tatttcttta gctttttcat cactatctac taatatatgt ttagcagttg cactttctgg
    14461 tttagagaaa gagctcttat tagcttcaaa gaattttact ttttcatctt ctgttacatt
    14521 aacactagat aaaactttat ttatagcaaa ttgctttaat acattttctt ctatcttttt
    14581 catttctaat ttaaatattt cttcttcatc taatttttct tctttagcat ctaagaagaa
    14641 taattcttga ttcactaaat catttaataa gtgttttttt ccttcttcag ttttaaattg
    14701 catagcttga taagggtcta atgattttaa tgcattttca atatcaatat tagttatctc
    14761 tttttctcca actgttgcta a
//
LOCUS       DAEPOZ010000091        14736 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.091, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000091 DAEPOZ010000000
VERSION     DAEPOZ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14736
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.091"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            775..2445
                     /locus_tag="KR440_001986"
     CDS             775..2445
                     /locus_tag="KR440_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HBH0699037.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYEEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESNKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
     gene            complement(2567..3301)
                     /gene="pflA"
                     /locus_tag="KR440_001987"
     CDS             complement(2567..3301)
                     /gene="pflA"
                     /locus_tag="KR440_001987"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBH0699038.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3461..5692)
                     /gene="pflB"
                     /locus_tag="KR440_001988"
     CDS             complement(3461..5692)
                     /gene="pflB"
                     /locus_tag="KR440_001988"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBH0699039.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPAALGKDMTERINNLSAMMDGYFS
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            5985..7046
                     /locus_tag="KR440_001989"
     CDS             5985..7046
                     /locus_tag="KR440_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBH0699040.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVVLFLVGFLLITKGADIF
                     INCTVEIGRKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLIVIIPTLKSNRLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            7137..8750
                     /locus_tag="KR440_001990"
     CDS             7137..8750
                     /locus_tag="KR440_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBH0699041.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            9025..11010
                     /locus_tag="KR440_001991"
     CDS             9025..11010
                     /locus_tag="KR440_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBH0699042.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLSFGILEKPVVWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMIEELAKVKSFDEFYKIITKRR"
     gene            11015..11449
                     /locus_tag="KR440_001992"
     CDS             11015..11449
                     /locus_tag="KR440_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HBH0699043.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKSLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            11478..12038
                     /gene="srlA"
                     /locus_tag="KR440_001993"
     CDS             11478..12038
                     /gene="srlA"
                     /locus_tag="KR440_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBH0699044.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            12102..12470
                     /locus_tag="KR440_001994"
     CDS             12102..12470
                     /locus_tag="KR440_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HBH0699045.1"
                     /translation="MAFNKVKIVKGSGGWGGPLIIEPTEKKNKVVYVTGGAKPETAVK
                     IAELTGCELIDGFSQGVKDDEIACVIINCGGTLRCGIYPQKKIPTINIMKTGKSGPLA
                     MFIKEDIYVSGVKPTNVEQL"
     gene            12495..13061
                     /locus_tag="KR440_001995"
     CDS             12495..13061
                     /locus_tag="KR440_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBH0699046.1"
                     /translation="MAYDTKKKISEQSQGIVAKIGIALGNVTSTLFQAGRDTIDTLIH
                     TILPFMAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFPLLSPFLGPG
                     AVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAEAEPDTVQVG
                     VPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            13102..13467
                     /locus_tag="KR440_001996"
     CDS             13102..13467
                     /locus_tag="KR440_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBH0699047.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTNDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            13568..14341
                     /gene="srlD"
                     /locus_tag="KR440_001997"
     CDS             13568..14341
                     /gene="srlD"
                     /locus_tag="KR440_001997"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HBH0699048.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIETEFGCKSVGYGCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
BASE COUNT         5652 a         1749 c         2478 g         4857 t
ORIGIN      
        1 tctgtagtgt aatcgtattt tttattaatt cttataaaat caatgaaaat aaggatatcc
       61 attttaaagt acataaattt aaaaatccag aaaaatatac ttaaattatg taagcaaaag
      121 aggggttgaa tcggctgaaa atgtaatact agtaaatagt acatcaatcc tagataccct
      181 gtaagcaact ctattttcat gtactaaaaa tgtacctatt ttattaactg aaaagaataa
      241 attaaatgta agtacataat aagagataaa aaattaaaag caaaaaatat ttatataaat
      301 aaatataaaa aatgatataa tccctattaa aaaatttata gatgatgaag aaatatcaaa
      361 atcttatatt ataggaagag gcacttcttt gccaaaatat atagaaaagc aataccaaat
      421 ccaaagagaa taggctgaga aaaaataaaa caaattcaag agtaatagaa aagctttata
      481 gaataaagaa ctgagaactc tttatataat aaaaaatgga cttttaagag aaaatgattt
      541 aataaatgat ttaacatggt gttttagcag gtgaaaataa atcaccagta atgattgtag
      601 aaaaattact ttatgaatct caaaaaaact tagttaagag taagagattt gataagataa
      661 ctaaagttgg ggaaatgtgt ttgatgagat aaaaaaatta gtaaaatagt ataatctaaa
      721 taagaatatt ttgttgatta aactacacta atagtaatat tgtaggggga aaagatgaga
      781 gaagaatcga actctttaag tttagaccat atagaaattt tctttgaact tttatctcaa
      841 agtacagaag attatatatt tttttgggat ataaatagga ataaatttaa aatttcttca
      901 gcaatttttg atgaatttaa cttaagtaaa gagatagaat ctgatttagt caattgttgg
      961 agtaaaattg tttatccgga tgatgttcaa atatggaaag atgatataca agaattacta
     1021 catggaaaaa aaggtgagca caatttagaa tataggttga ttaataaata tgaagaaata
     1081 gtatggattt cttgtagagg taaagtttat gtgtcggatg acccaaaaac tatatttcta
     1141 gtcgggagaa taaagaatat tggtgaaaaa aataaatttg attctatcac aggaacgtgg
     1201 aatagagaac aatttgaaca tagaatgaat tatcttataa aagagaaaat ttataaaaat
     1261 ggtgctatgt ttatcatgga tattgataat tttaaaaata taaatgaaaa atatggacat
     1321 tcttatggtg acaaagtatt gcgtgcaatt gctactgaag tattagaata tcttccaaaa
     1381 gatgtaagat tgtataggtt agatggagat gaatttgcat ttttttaccc tatgtgtact
     1441 aaagaaacta tagagaaaat atatgaaaag atacagatgt atacaaatac tcagcatgaa
     1501 attgaatcaa ataagtacta ttgcacagtg actgcaggag ttgctatgta tccagaagat
     1561 ggagataatt atttagattt atttaaacat gctgatattg cattggatat agcaaaaatt
     1621 agtggtaaaa atagaattaa gtttttttca caagagctct atgaaaataa actaaaggta
     1681 atttcaatgc aacaaaaact aagagagtgc gttgaaaata attttaatga ctttgagctg
     1741 ttttttcaac cccaggtaaa tgctgttaca aaagaggtaa taggggcaga ggtactttta
     1801 agatggcatt cgagtactta tggagaagta tctcctgttg aatttatacc aatactagaa
     1861 cagagtaatt taattatacc agttgggaag tggattataa aagaagctgt aaaacagtgt
     1921 aaagaatggc ataaaataaa tccagacttt aaaatatctg taaatgtatc gtatattcag
     1981 ttaaaagaag atttttttag agattttata gtagaatgtt tagtagagta tcaattaaga
     2041 cctgaatttt taattttgga attaacagag aattgttgga taccagatat aaatttacta
     2101 aatgataaat ttattagttt aaagggtata ggagtatata ttgcaataga tgactttggt
     2161 acagggtatt catctttaaa ttaccttaaa gaactgtctg ttaatataat aaaaattgaa
     2221 agaagttttg ttaagaatat tacatataat agttatgaat atacttttct tgaatatatt
     2281 attaaattag ctcatattat aaatttaaaa gtttgtgttg agggaataga gtcttatgaa
     2341 gagtatgata tagtaaaaag cttaggtgta gatattattc agggtttttt atttggaaga
     2401 cctgtaagtg cctctgaatt ttataaattt caactatcaa agtgatgtac ataaaatgag
     2461 atatatagta tataaaaata gggcaaagtg gaagttttga agtttattaa aaatttacac
     2521 tttaccctat tttgttatgt cttctataaa ggattaatat actacactaa ctattatata
     2581 ctttcactcc aaatttagag attatttcac tagctttttg gacatcttcc ttagtggcat
     2641 caggaacatc ttttagttca taatcgatac ctaaactttc ccatttgtgt attcccatag
     2701 aatggtatgg tagcaattca aatctttcta catttttcaa tgtagctaca aattgaccta
     2761 gtttttctaa attatctata tcatcagtaa tgccaggaac taaaacatgt cttatccata
     2821 caggaatatt tcttgattct aagtgtctag caagttttaa tgccttatcc ataccaacac
     2881 cagtcaaatc aatagatttt tcctcaacca tatgttttaa atcaagtaaa accaaatcag
     2941 tattatctaa aactgggtct atagtttcta tatctacgaa tccagatgta tccaagcaag
     3001 tgtgtatatc attttctttt gcttttttaa ataattcact taaaaattct ggttgaagag
     3061 tggattcacc accagaggca gttatacctc caccagaaaa cttcataaaa gaagtatact
     3121 taagagcatc cgttattatt tcatcaactg tgtattcttt tcctgattta atatcccatg
     3181 tatccctgtt atgacagtat ttgcatctaa gaggacaccc ctgaaaaaat aatatatatc
     3241 ttattcctgg tccatctaca gtaccaaacg tttctataga atgtatcttt cccttaatca
     3301 tagagttcac ctcacaatat attttaataa ccttatcaat agttataata tttgtctaaa
     3361 aaaggccatc ttataccaga aataattcaa atataagata gccttttatt tttatttttt
     3421 acacagtagt aatcttagtt aaagtataaa aatttaaaga ctaagccatt ttaccgtgga
     3481 atgttctgtt tataacatct aattgttgct ctttagttaa ttttatgaag ttaactgcat
     3541 atccagatac tcttatagtt aattgtggat attcttctgg atgttccata gcatcttcta
     3601 aagtagctct atcaaataca tttacattta agtgatgagc attttgagag aagtatccat
     3661 ccatcattgc agaaagattg tttattcttt cagtcatatc ttttcctaaa gcagcaggta
     3721 ctatagagaa agtattagat ataccatctt gagcatgttc atatggtaat ttagcaactg
     3781 aagataatga agctaaagca ccattagtat ctctaccatg cattgggtta gcaccaggag
     3841 caaatggagc accagctctt ctaccatctg gtgtattacc agttttctta ccataaacaa
     3901 catttgaagt tattgtaagt atagattgag ttggataaga atttctataa gttttatttt
     3961 ttcttatttt attcataaag ctttcaacta actctacagc tatactatca actctatcat
     4021 cattgtttcc gtattttgga taatctcctt ctatttcaaa atcaacagct attccttgtt
     4081 catttctaat agttttaacc ttagcatgtt ttatagcaga taatgagtct gcacaaactg
     4141 ataacccagc cataccacaa gccatagttc taaatatatc tctatcatgt aatgccattt
     4201 ctaatgcttc ataagaatat ttatcatgca tataatgtat tacatttaaa gtatttacat
     4261 ataatgttgc taaccaatca gtgaatggtt caaatttact cataacttca tcataatcta
     4321 agtactcaga agttataggt tcaaatcttg gtccaacttg aacaccagat ttctcatcta
     4381 caccaccatt tatagcgtat aataaagttt tagctaagtt aactctagct ccaaagaact
     4441 gcatttgttt accaattctc attgcagata cacaacaagc tataccatag tcatctcccc
     4501 agtatgctct cattaaatca tcattttcat actgtactga acttgtatct atagatactt
     4561 tagaacagaa atctttgaat ccttgaggta atttagttga ccatagaact gttaagtttg
     4621 gctctggaga aggtcctaat gtatatagag tatttaacat tctaaatgag tttttagtaa
     4681 ctaatgttct accatctatt cccatacctc ctatacactc agttacccaa gttgggtctc
     4741 cagagaatag gttattgtaa tcaggagttc ttaggaactt aaccattctt aatttcataa
     4801 caaaatggtc catatactct tgtaattctt cttctgttac gattccagct tttaaatctc
     4861 tttcaaagta tatatctaag aaagtagatg ttctacctaa agacattgca gcaccatttt
     4921 ggtctttaac agcacctaaa taaccaaagt ataaccattg tacagcttct tttgagttag
     4981 ttgctggttt tgatatatca aatccataag attcagccat ttttttaagt tcatttaaag
     5041 ctgatatttg ctcagttatt tcttctctta atctgataac ttcctcatcc atgcaagaaa
     5101 cttctaatga tttcttttgc tcatttttat cttctattaa agcatcaaca ccgtatagag
     5161 caactcttct gtagtcacca attattctac ctctaccata agcatctgga agacctgtta
     5221 ttataccaga ttttctagca gctctcattt ctggagtata aacatcaaaa actccttggt
     5281 tgtgtgtttt tctgtattta gtaaatatat cttttatttc tgggtctaat tcatatccaa
     5341 aagcttcgca tgaattttca accatcttta taccaccata tggcatgaca gctcttttta
     5401 aaggggcatc cgtttgtaac ccaactattg tttctaattc tttatctaaa tatcctggag
     5461 catatgcagc tattccagaa acagtttttg tatcaacatc taaagtacca ccattttctc
     5521 tttctttttt aaataggacc attgcttcgt cccataattt tttagtattt tcagtggcac
     5581 cagctaagaa agagtcattt ccctcatatg gagaatagtt taattgtata aactctctaa
     5641 catttatttc tttagtccat cttcctgttt taaatccttg ccatgcattc attaagtatt
     5701 tcctccttag atttaaatga taaataatac tattaaaatt attttttata tacttaattt
     5761 attttatata tttttttaaa aatatatttt ctaatataat aatatcatat tattcgtatc
     5821 ctgtatacat aaaaaataaa aaattgtcgc tttaaagtat aaaacagatt atatgttaat
     5881 aatgtggtga aattataccc atatataaat aaaataaaac aaataaatag ctaattaagg
     5941 agaaaatatt ttttattttt tgtttaggat aaataaattt tattatggaa aaaagaaaaa
     6001 gcatacatct tcacaaattg aatatttatt taataggggg aggtgataga ttgtttatca
     6061 cagtggtatt atttttagta ggtttcctat taattactaa aggcgcagat atctttataa
     6121 attgcactgt agagataggt agaaaaacga atatatcaga aattatactt ggagctacta
     6181 tagttagttt tgcaacaaca ttacctgaat ttacagtgtc tctacttgct tctattgatg
     6241 gtcatactac tatgagttta ggaaatgcag ttggttctat aatatgtaat acaggattag
     6301 cattagggtt ggttgttttt ataagtccat ttaatgtaga taaaaaaatg tttttttcta
     6361 agtctttact attaatagta tccgttatag ttttaatttt attgagttta gatggagtta
     6421 taacaagagg tgattcatta ttacttataa taatattaat cttttatatg gttaataatt
     6481 atagaagtgt cgttggaaaa tctgatacta aaaatagaaa tattaaaaat aacactatta
     6541 aagacatatc tactaaaaaa gggaaaaatt atagaggttt ttccgtatta gaaattgtaa
     6601 aaatactatt actttttgct acaggattga taatgatgat aataggttca caaatcctta
     6661 tagagagtgg agtaataata gctagttttt taaatatacc tcagggtata gtaagtttaa
     6721 ctataatagc acttggaact tctctgccag agatagtttc gtcgattact gctataagaa
     6781 aaaatcatca tgaaatatca gtaggaaata ttttgggtgc aaacatattg aatatagtat
     6841 cagtaatagc tgtatcagca ataccaaata acataccaat actatctcaa aatagacaat
     6901 tggatatacc attcatgata ttattattat taatagtaat aataccaaca cttaaatcta
     6961 atagacttag tagaatacaa ggtatattga tgttattcac atattttctg tacatatcaa
     7021 ttttgtattt catgtatatt atttaactat actgtttaaa atattaattg cagaatatcc
     7081 taaaaaagta tataatggat aggttaagat taattgataa cggagaggaa taatatatga
     7141 atataacaaa gtttgaagat ttaccaataa gtgagggaat aaaaaaagct atagctgaaa
     7201 tggggtttga agaaccatct cctatacaag cacagtctat acctgcgatt ttgtcaggta
     7261 aagacgttat aggacaagct caaactggta cgggaaaaac agctgctttt agtatcccta
     7321 tactagaaac aatagaccca aataatagaa gtttacaagc tgtagtgctt tgtccaacaa
     7381 gagaactggc aatacaagta tcaacagaaa taagaaagtt agctaagtat tcgcacggaa
     7441 taaagacatt acctatatat ggtggccagc caatagatag acaaataaaa tctctaaaga
     7501 gtggtgtgca agttgtaata ggtacaccag gacgtacaat agaccatatc aatcgtaaaa
     7561 cattaaagat ggataatgta aaaatgatta tattagatga agcagatgaa atgctagata
     7621 tgggatttag agaagatata gaaatgattt taagtaagat tccagaagaa agacaaacaa
     7681 ctttcttttc agctactatg ccaagaggaa ttttggaatt aactaagaga tatcaaaaag
     7741 acccagagca tataaaagtt gttagaaaag aacttacagt ttcaaataca aagcaatact
     7801 atatagaaac taggtcatca aataaattag aagtattatg tagattagta gatgtatatg
     7861 accctaaact atctgttgtt ttctgtaata ctaagagaaa agcagatgaa ttggtaggag
     7921 atttacaagc aagaggatat tttgcagatg cattacatgg tgacttaaaa cagactcaaa
     7981 gagatatagt aatggataag tttagaaatg gtacaataga tatacttgtt gctactgatg
     8041 ttgcagctag aggtatagat gttgatgatg ttgaatgtgt attcaactat gacttaccac
     8101 aagatgaaga gtattacgtt catagaattg gtagaactgg tcgtgctggt agagaaggta
     8161 tgtcatttac ttttgtattt ggaaaagaaa tgagaaagat gaaagatata gaaagatata
     8221 ctaaatctaa actaataaag cataatatac caacaataac tgatgttgaa gagaaaaaag
     8281 taggtacttt ctttgcacaa gttaaacaaa ctatagaaga aggtcactta actaaacaat
     8341 tacaatggtt agaaggtttc tgtaatgatg aagattatgc aatggttgat atagctgctg
     8401 cacttgtaaa actatctcta ggtgaagaaa tgaaagaaga aataatagaa gaaaaaccta
     8461 gaagagagcg tggagataga aaaggtggta ctggagctaa agatggtatg attagattat
     8521 ttatcaatat tggtagaaat caaagagtac aagcaaaaga tatagttggt gctatagctg
     8581 gagaagttgg aataccaggt aaagtagtag gtactataga tatatatgat aagtatactt
     8641 ttgttgaaat acctaaaaaa gatgcaaaaa cagtaattga aaagatgaaa gatattaaga
     8701 taaaaggtaa taagattaat atagaaaaag caaataagaa gaaaaaataa cgtatagtat
     8761 acaaaactat aattagaaaa tatataaaat aatatattgg atatttaaaa agcctgatgt
     8821 gttttcaaaa cagatagtag ataatctgta agagttaaca gcataaggct tttcttgttt
     8881 tatatgatat tatgattttc cagatcgttg tgaaaaatgt atattagttc ctttttttca
     8941 aatctgattt gaaaaagaga atattaatta tctatatagt ttagattata ataaaaaatg
     9001 taagataaca aaggggagaa aaagatgtca atgacttata tggaccctag ggtttttttg
     9061 attttagaaa tggtgttaga agaagacaca aatactctag aacaattttc aaatgttttg
     9121 ggtgttagta ctaggactat cagaaactat ataaaccaga ttaatcgaga attaaaaggt
     9181 ggtattgcta aaatagttaa aaatgaaaaa ggtacatata gtataagaat tgaggatgaa
     9241 agtaaattaa ttgagattgt aaattttaat agaggaaagc tatcaaattt tataaattta
     9301 aattcacctg atgaaagaat aaattatgtt cttgatattt taactatgac caacaatcca
     9361 attaccatag atgatttggc tgaagagata tgtataggaa gaacaacatt aataaaggat
     9421 ttaaaaaaag cagacagaat tttagctgaa tatgatttag aattaaaaag gaagccaaat
     9481 ataggtatga ttttagttgg tgaagaaatg gatataagat tattaatcct tgatagattg
     9541 tatgaaaatt atattgatgt cttggaaaac gttaatcaga taaatttagt taaagattat
     9601 gatattgaat gtcttagaga tgaattaaaa aagttgttta aaaaagaaga actctacata
     9661 acagaacagg tcttgagaga tgttgaaaga tatataatca tgtctgtctt aagaaatcta
     9721 aatgggtaca aatttgaaaa gatagataag agatttgaag tgataagatg ttcagatgaa
     9781 tatactttag gattaaagct caaagcttta ttggaaaaga tatttaatat agttttaaat
     9841 gaaaaagaaa ccatattttt gacaatgcca ttaataggta gaaaagctcc tagtacaaaa
     9901 ttagcactta gcagtataaa gataaatgat agcgtaaagg aagttgtaga tgaagtaact
     9961 agttttttat tagagacctc agggattgat tttaaagaag ataaaaagct tatagaaaat
    10021 ttggaatatc acttgtattt tgcacttaat agaatgagat tcaacataag ggtaaaaaat
    10081 cctcttttac aagaaataaa ggaaagatat acatttccat atagtgttgc aaagatagct
    10141 gcaatgataa ttgaggaaaa gtttgacttg aaaatatgtg accacgaaat aggatatata
    10201 gcattacatt ttggaagtta ttttgaaaaa aattcaagaa aagttctaaa tgtaaatagg
    10261 gtagctgttg tttgtggaac aggtcttggg actgcacaac ttattagaat aaagatagga
    10321 aaaatacttg gagaaagtgt agaagtaaat actttttctg atatagaagc taaagctaat
    10381 ttagatttat taaagtatta tgatttagta tttacaacag tagatttcaa aacagactta
    10441 gatgttccaa tttttagagt aaatgcgtta tttaatgaag aaagtataaa gaaagagata
    10501 gaaaatgcaa tacttttgaa aaatagtata aatcttaagt ctataaaaaa gattgaaaaa
    10561 aatatccctt ttataggtaa attaattgaa gaggataagt tctttatatt gaataagagt
    10621 acgtttatgg aaaatttaga agatatgtta gaaaaattag ttcataaagg aattattgat
    10681 atagagttca aaaaaaaggt attaaaaaga gaaaagaaat cttcaacagc attagataat
    10741 tatatagcat tgcctcactc agttaatact aatggggaaa atctttatct gtcatttgga
    10801 atattagaaa aaccagtggt ttgggataca aaggaaatta gaataataat attgatgata
    10861 attccagata agaatgtaga tagtacagac ctgataataa aaagttatga agaagtactt
    10921 gaagtaggga gagataaaaa aatgatagaa gaacttgcta aagtaaaatc atttgatgaa
    10981 ttttataaaa taattaccaa gaggaggtaa taatatggac ccagtaattt ttattatagc
    11041 atcacttatt acagtgtatt tattaaatat ttttcttgga tacttacagc ttaaagattt
    11101 taataaaaat tatattgaac taaaaagaaa aggaagagtg gcaataggca gaaaaaaagg
    11161 aagaatttca tcaggtacaa ttgtgttgat acttataaat gatgatggtg tgattgtcga
    11221 aactagaaaa atgcaaggtg ttacagtatt ggcaagagta aagtcatttg aaggtttggt
    11281 aggaaagagc ttagagtaca tagaaaaatc agatttaact gtttacaata aactacttaa
    11341 aaaagcaata ctagatgcag tcaaacagta cataacattt gagaaaaata aatgtgaaga
    11401 tacaaataat ctgagtactg atgaagagtg taggatggaa gtagtttaaa aaaatatatt
    11461 tataaggagg agttaatatg acgcaatttt ttcaagtatt agcaagtggt gcagatagtt
    11521 ttatgaattt atttagggca ggtgggcaac aatttgtagg ttttgtaaca gatatagtac
    11581 cacttttagt agcattatta gttacaatga atgcaattat caactttatt ggtacagata
    11641 gagtcgagag acttgcaaaa aaatgttctg caaatatatt tactagatat ttgatattac
    11701 cagtccttgg tacatttgta tttgcaaacc caatgacact atcactagga agattcttgc
    11761 cagaaaagtt taaacctagt tactttgcag cagcaagctt ttcatgccat acaatgaatg
    11821 gattattccc acatatcaat ccaggtgaac tatttatata tctaggaatt gcaaatggta
    11881 taacaacttt gggattctca acagcagatt tagcagttag atatttgtta gtgggtatag
    11941 ttgcaaactt tattaaaggt gtagttactg actatacaac aaaatttgta gagaaacaac
    12001 aaggaatagc attaaattca gatataaaga ttggataatt actttaaggt ataaataaat
    12061 ttttcaaata aaaagatagt gtatagagga ggataaatta tatggcattt aacaaagtta
    12121 aaatagtaaa aggttctgga ggatggggtg gaccattgat aatagaacca acagaaaaga
    12181 agaataaggt agtatatgtg actgggggag caaagccaga aactgcagta aaaatagctg
    12241 aacttacagg atgtgagtta atagatggat tttcacaggg tgttaaagat gatgaaatcg
    12301 catgtgtaat aataaattgt ggtggaacat tgagatgtgg tatatatcct caaaagaaaa
    12361 taccgactat aaatataatg aaaacaggaa aaagtggacc attagcaatg tttataaaag
    12421 aagatatata tgtttcaggg gtaaaaccaa caaatgtaga gcagttataa ttgaggtagt
    12481 aggagggaag aaatatggct tatgatacta aaaagaagat aagtgagcag agtcaaggaa
    12541 ttgttgcaaa aataggtata gcactaggaa atgtgacaag tactcttttt caagctggta
    12601 gggatactat agatacatta atacatacta tactgccttt tatggctttc gttgcagtcc
    12661 taataggtat aataaatcaa tcaggtatag gaaattgggt agccaattta cttactccat
    12721 taatcggaaa tgttggaggt ttagttgtct tatcaataat atgttcattt ccgttgttgt
    12781 ctccgtttct tggaccagga gctgttatag ctcagatagt gggggtattg gttggtacag
    12841 aaataggaaa aggaaatatt ccaccaagtt tagcattacc agcattgttt gcaataaact
    12901 cacaagcagc ttgtgacttt ataccagtag gtcttggtct tgcagaagct gaacctgata
    12961 cagttcaagt aggagttccg tcagttttat attctagatt tttaacaggt gcaccaactg
    13021 ttctattagc atggttagca agctttgggt tatataaata gaagaatact ttataaataa
    13081 ttagagagag gttcagttga tatggaatta atatacagta ctcaaataaa taaaatagga
    13141 gataatgcaa atgaattttt acaacataat atgtttgtaa ctttcaaaga taatgctcct
    13201 caagagttag aaaattactg ttatatacat tctgaaaata atttagtaaa agaaatactt
    13261 acgaatgatg tgttatcaat aaataatgtt gattacaaaa ttacttctgt tggtgagtta
    13321 gtaaaccaaa atctttcaga attaggtcat ataacattta agtttacagg tgaaaaagat
    13381 ggagcaattg gagggacctt atatcttgaa gaaaaagaaa tagcacctat tggcgtaggt
    13441 actatactaa aaataattag aaaataaaat ttaaaacgaa tctaatcata ttataagtaa
    13501 attatagata aaagtcaatt taactaatca agttaacttt taatgtgata aaaaagggga
    13561 aatgaaaatg agaaaagtgg ctttagttgt tggaggagga agaagtttag gtgaatattt
    13621 atctaaacat ttagctaaag taggttatga tgttgctatt gcagatttaa atgaagacaa
    13681 cgcaaaaaaa gtagctgaag aaatagaaac tgaatttggt tgtaaatctg taggatatgg
    13741 ttgtaatgcc atagatgaaa gacaggttat aagcactgtt aaagctattg aaaatgattt
    13801 tgatagaata gatttattag tgtacaatgc aggagtggct gtaagtaaaa aaataactga
    13861 ttttggatac aaagatttta aatttattgt agacgtaaat ttaaatgggt actttctatt
    13921 tgcaagagaa gtatctaaag tcatgatagc aaaaggtatt gagggtagaa tcatacagat
    13981 aaattcaaaa tcaggtagag tgggaagcaa gcataattca ggatattcag caggtaaatt
    14041 tgctggagta ggacttactc aaagtttagc gctagatttg gcagaacata agataactgt
    14101 aaatgcactg atgctcggta atttacttga ttctgatatg tttgaaagtt taattcctca
    14161 atatgcagta aaattgggaa tacctgaatc agaagtcaag caagtttata tagataaagt
    14221 acctttaaaa agagggtgta gatttataga tgttgcaaat atgcttacat tttatgcttc
    14281 aaaggaagct gaatactgta ctggtcaatc tataaatatt actggtggac aagttatgta
    14341 aggtttttat ggagtgtctc aagatgaact ctttagttca tgaggcactt tttttgtata
    14401 aaattaaata tattttcatc atttaaacaa tgaaaaagag gtttatcttt attttgagat
    14461 agcctcattt ttgtaattaa gaaacatagt gtttaagtaa acatataaga ttgttagttt
    14521 tttaaaagag aattttttaa taatattata tttatgaagt atttttaaac ttaatttgat
    14581 taaaaaatag ctacgcacac ttgtgacttt agtcatgagt tagtagctaa attagtcagc
    14641 atatagggaa acttatatgt agagatagga tagaacctat ccaatactac ttgaattgct
    14701 aaaaacccct aaagctaacc aaactaaaac ataagg
//
LOCUS       DAEPOZ010000092        14541 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.092, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000092 DAEPOZ010000000
VERSION     DAEPOZ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14541
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.092"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            260..871
                     /locus_tag="KR440_001998"
     CDS             260..871
                     /locus_tag="KR440_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699049.1"
                     /translation="MNIKRINLLLSTVMAAVIISLPLAVTANAMEKTESDNNVKIELS
                     DKEVEELHNYWPEDKIFSDEKIDEILIKTGQFTQKELDKINQEVDDSAKKVNQNSRVK
                     YINGYNKYRTFTKNGKNYLYIYVSGKTLKKIKAGTDLASTFGGFLPKIWVELDVVAIG
                     KIITINMDGINTNYGIVLSYIEDKWNFQGATYTYRYDHWFYQT"
     gene            899..1081
                     /locus_tag="KR440_001999"
     CDS             899..1081
                     /locus_tag="KR440_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699050.1"
                     /translation="MKKRLYKVFLKTIPNLIIVILSLALSFYTNTFDIDLKILLLIPL
                     AYVLLFIYYYIKTKKD"
     gene            1321..1863
                     /locus_tag="KR440_002000"
     CDS             1321..1863
                     /locus_tag="KR440_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH0699051.1"
                     /translation="MLIYLAILELEEDKIKFEKVYEKYRQIMFYVANKILKDDHLSED
                     AVHNAFLRIIKNIDKIDEVDSPRTKAFIVIIVERIAIDFYRKRKREKVSDIEEEYKNR
                     EINFSIEDKVCESNLAIALAKLNESYFQVLSLKFQYGFSNKEIANALNLGEENVYKRI
                     QRARKKLKEILEEMEVGNIE"
     gene            1895..2557
                     /locus_tag="KR440_002001"
     CDS             1895..2557
                     /locus_tag="KR440_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HBH0699052.1"
                     /translation="MLRESIPIATKIIIDDLLSDDELNHVFSKGFERKMKKLIKQQKR
                     SSLTNKIIFYSKQTAIVFLIVLASLLTITMSSEALRSRFFRLVIRVYEDLTSFVFSTN
                     GEDNLSLKNKEPEYIPKGFKEVDRVDDFVITYRNDENIEIQYVTDKISSNSIILDTEN
                     AKVKDIFVNGHKAKYIVKGTFLQLFWNDNNFIYLLNVDYQNSGESEFYEDILIKIAKS
                     IK"
     gene            2747..3064
                     /locus_tag="KR440_002002"
     CDS             2747..3064
                     /locus_tag="KR440_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699053.1"
                     /translation="MKSDLYLCKSNKLYRDNEVLEKFMEDLSFLIDDYKFSLETISIL
                     CKINIEKLTNFYAGKGYILYEEMSLIESVLAPFLSAIDIAKHNYDVVIKSRESDKPLI
                     FEN"
     gene            3086..3529
                     /locus_tag="KR440_002003"
     CDS             3086..3529
                     /locus_tag="KR440_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699054.1"
                     /translation="MKRLFALLLTLSLVILPSTLVFANEKAENDSIYAEAPLVFQSNE
                     KAYTHGSGGTAYIGYMSSKHLNWRITSSKGIVILFSGTVEIRNSKNMLVASYPISGGG
                     SSTISGQIDVKYLKKGDYKAILKGTSTTGGGVLVVKSGLVQMFTI"
     gene            3747..4190
                     /locus_tag="KR440_002004"
     CDS             3747..4190
                     /locus_tag="KR440_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699055.1"
                     /translation="MNLRKTVISGMCALVCSSAVAIPAFADEYQDKNIEQYSAVNYID
                     DTMVKMSYIKSGTCNLSISGSTAKVTCFVRGASNVTSTSIIARLQKSNDNGKTWSTIQ
                     TWNASGNISCSLSKSKTIGKGYYRVHSTIKANSESKNIISSTKKY"
     gene            complement(4321..4741)
                     /locus_tag="KR440_002005"
                     /pseudo
     CDS             complement(4321..4741)
                     /locus_tag="KR440_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894307.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            complement(4719..5996)
                     /locus_tag="KR440_002006"
                     /pseudo
     CDS             complement(4719..5996)
                     /locus_tag="KR440_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899679.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(6019..6561)
                     /locus_tag="KR440_002007"
     CDS             complement(6019..6561)
                     /locus_tag="KR440_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699056.1"
                     /translation="MLLCDPASAGGSKHDYSAFLVGSESENGLLCGRLAELAKINART
                     DFDKYILHMIYLLKVYPDITHVYIEKNTFNGTAANQLELKIKNDDVLYYRDIEIINEH
                     QKKNKDDKISTLIPVLNKGQMIFAEEDKAFIQQILNFTGQKYSLHDDAPDISAEFINR
                     IFNIKVNESITLLDRRNLGL"
     gene            complement(6601..6819)
                     /locus_tag="KR440_002008"
     CDS             complement(6601..6819)
                     /locus_tag="KR440_002008"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699057.1"
                     /translation="MLFDFKNTNHLEDAKEFYWENEKEIKFPILWPSFWNCLDMALSY
                     YENPTSFKQEVQNDVNSIGEKWFKTIRT"
     gene            complement(7015..7758)
                     /locus_tag="KR440_002009"
     CDS             complement(7015..7758)
                     /locus_tag="KR440_002009"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699058.1"
                     /translation="MDNLERNIQLIFSYMAKTFIRNGATIREAEKEANEIIKTNSSNL
                     WGVNGLAYQLGKINLEFFCMYFIQDTYLPKEDNVAAPIAKVHHELWEDIQESIIGDGS
                     QQLGRIYPRGTGKSAFGDLATTVWSHCYKHKTYTLICSDIGSTAEKFVKDIKNALLEN
                     EYIKKAFGVLLNDNDRKYICNSTQLELTNKTFIEAISSSSPMRGKKYNNNRPDLIILD
                     DYQSEENVRTEDAREKKFKKFSDDVKYAA"
     gene            complement(8058..8615)
                     /locus_tag="KR440_002010"
     CDS             complement(8058..8615)
                     /locus_tag="KR440_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH0699059.1"
                     /translation="MVVGYMRVSTAEQNEDRQLVTMKKHNVEKMYQEKISAKDTKRSK
                     LNAMLDFVREGDTIIVHDFSRLARSTKDLLEIVELLEKKHVKLISDKENLDTSTPTGK
                     LMLTMLGAIYEFERTNMLERQREGISIAKEQGKYKGRKEVKIDNFGSYYDRYKTRELN
                     KTQLAKELGVSRPTLDRLIKSHESN"
     gene            complement(8810..8998)
                     /locus_tag="KR440_002011"
     CDS             complement(8810..8998)
                     /locus_tag="KR440_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699060.1"
                     /translation="MNNKKKSFLISGTINVILLIIVFALFSYNIIENSTRNMIITVIL
                     IFIFEIIKIKILEKYYNL"
     gene            complement(9009..9584)
                     /locus_tag="KR440_002012"
     CDS             complement(9009..9584)
                     /locus_tag="KR440_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699061.1"
                     /translation="MLKKKIISFLMAGIFVLSSGYNLVYASTFEENEYKQELVNENEF
                     TIINSNENEMIVDKKGNNTYVSTKVTPGSWKNVKYLGQKSRKLSSPYTRERIIQTIQW
                     GLGYLSGGSSFLLGLAESIYREKVYDITKYGGVYTKTYYRRQITDIRNSHANVPVPYR
                     YEYVTYFYSSSNFKKVVYTERVGDGIKLANN"
     gene            complement(9917..11056)
                     /locus_tag="KR440_002013"
     CDS             complement(9917..11056)
                     /locus_tag="KR440_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBH0699062.1"
                     /translation="MVIKLTIEEYKNKYSTGDKIILSLKSFDNKELYYHETDNILNSK
                     DEIDRLTSSKIFKIIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYM
                     IYNNHRALEYGLDNLDKLYSHKFIYNLHHILGENCLDSEEYEYRTEKVYVCDSKGEII
                     HTGLDPLKIYNFMDELIIFMENSKVNDLVKSTIIHFYFVYVHPFSDGNGRTSRALSYL
                     YLINKGYDTFKEFSISYIISKNRTKYYKAILDVENKGYNLTVFIEFMLKSIIQSINEM
                     RDMHDRKSLESILKEELFENDITLSATEEHILKYICSKDNYSMTLENYIKKNKGRYLK
                     AGIKEIELVDQLMEVFNNLEEIEILSREKDVYKVNEKYLNMLEIS"
     gene            11393..12646
                     /locus_tag="KR440_002014"
     CDS             11393..12646
                     /locus_tag="KR440_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HBH0699063.1"
                     /translation="MLNKKNISSIIMTMILSLSTTVFVFADENTNVKGQDVTKYNEEM
                     VSKNLEDLSIEQLIDIRDKAIDKNLEGLRKFGEDSVKERKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQINIVEYENSINDIMDPSGDFFKVTNQSYQINEFVLKNGKNYKEWSY
                     EYVEFLIEHLEDLCNFVDFDAKYVIEMVDPSIKPDLSDNQKKSLNNELIKMYSSNTLS
                     TNIKDEIENWENNLNINEKNSNEPLPRVSPSSYRQQALAYAKKHGYTNGYYGSTNRKN
                     NAPSPYYNFQKDGYGDCANFVSQCLHEAGVTFWTNNNPWYYYSTSNRSPSWAGAREFK
                     IHWMNRIAYQTLTVSNSLSFLRPATPVSILNSSGVATHTLISTDKHSNGYNFSYAAHS
                     DEGARTNLLKKLEGKKISYYKVYWE"
     gene            12980..13711
                     /locus_tag="KR440_002015"
     CDS             12980..13711
                     /locus_tag="KR440_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699064.1"
                     /translation="MLNKKNVSLIVLVIVLSLFTAIFVFNNRNTNDKSKEIAKNNEEI
                     VPKNLEALSIEQLTKIRDKAIDKNLEGLREFGEDSVKQRKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQINIVNYENSINDIMDPSGSFFELANQSYEINEFMLKNKKNYKEWSY
                     EYIEFLIDHLDELCKFVDFDIKDVIDIIDPTIKTDLSEEQKKSLNDKLKKVSSSDTLS
                     EDIKKEIKNWENNLNSLNMNKNNSN"
     gene            13953..>14541
                     /locus_tag="KR440_002016"
     CDS             13953..>14541
                     /locus_tag="KR440_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBH0699065.1"
                     /translation="MFNKNEILKKLIEEYDVKTTTDIQDMLKDLFASTIHQMLEAELD
                     DHLGYDRYGNKNKNTKNSRNGYRNKNVKSDFGEITLNIPRDRNGDFEPKVISNYKNDI
                     SGIENQVIGMYSKGMSTRDISSHLKSIYGIDISHSLVAKITDRVLPLAHEWQNRPLDM
                     VYPIVFMDAIHYKVRSEGRIINKAAYIAIGVNLEGI"
BASE COUNT         5374 a         1846 c         1855 g         5466 t
ORIGIN      
        1 tcaattaaaa actgaactat attaaaatta attttataaa taacaaatat aaataattac
       61 aaaaccttaa ctgcgttaag tttgaaatat aataatatta aaaacacttc tctcaaatca
      121 agtatctttt gttatatttt cataagtttt aaaaatataa attttttaaa acttgtgtcc
      181 aaaaaacaaa attaaatatg ttatatatgt aaagggttaa ttttaaaacc ttaaatttat
      241 tatagttagg gggatttaaa tgaatatcaa aagaattaat ctccttttat caacagtgat
      301 ggcagctgtg ataatatctt taccacttgc tgttacagca aatgcaatgg aaaaaacaga
      361 atcagataat aatgtgaaaa tagagttgtc tgacaaagaa gtagaggaat tacataatta
      421 ctggcctgag gataaaattt tttcagatga aaagattgat gagatcttaa ttaaaacagg
      481 tcaatttact caaaaggaat tggataaaat caatcaagaa gtggacgatt ctgctaagaa
      541 agtaaatcaa aattctcgtg tgaaatatat aaatgggtat aacaaataca gaacttttac
      601 taaaaatggt aaaaactatt tatatattta tgtaagtgga aagacattaa agaaaataaa
      661 agctggaaca gatcttgcat ctacttttgg aggtttttta ccaaaaattt gggtagagct
      721 tgatgtagtg gcaattggaa agataataac aattaacatg gatggtataa atactaatta
      781 tgggattgtt ttatcatata tagaggataa gtggaatttt cagggtgcaa catatactta
      841 tagatatgat cattggttct atcaaactta aaagacatgc ttttttagga ggaataatat
      901 gaaaaaaagg ctttataagg tttttttaaa aacaattcca aacctgataa ttgtgatatt
      961 atcacttgca ttatcatttt atactaatac atttgatatt gatttaaaaa tattgctttt
     1021 aataccttta gcatatgtac ttttgtttat atactattac atcaaaacaa aaaaagatta
     1081 atttttatgg aattagatat atacgaaaat aattaattta aaaatttaac tttgagaatc
     1141 atttgtagag ttaaggccct tataatcccg aaaatggatt tgaagggcct ttttactaga
     1201 tatctttaaa aagcttgtta agcatagtta tattctattt aagtagagtt aattatatat
     1261 ttatgttata attgaacttt aaaatattaa ttcagaattt ttatattgga ggaattctaa
     1321 atgttaattt atctagctat acttgaactc gaagaagata aaataaagtt tgaaaaggta
     1381 tatgaaaagt atagacaaat aatgttttat gtagccaata aaatattaaa ggatgatcat
     1441 ttatcagaag atgcagtaca taatgctttt ttaagaataa ttaaaaatat agataaaatt
     1501 gatgaggtag acagtccaag aacgaaggct tttatcgtta taatagtaga aaggattgct
     1561 atagactttt acagaaagag aaaaagagaa aaggtatctg atattgaaga agagtataaa
     1621 aacagggaga taaatttttc tatagaagat aaagtatgtg aaagtaattt agctatagca
     1681 cttgcaaaac ttaatgaaag ttactttcaa gtgctttcct taaagttcca gtatggtttt
     1741 tcaaataaag aaatagcaaa tgcattaaat ttaggggagg aaaatgtata taagagaatt
     1801 cagcgtgcta gaaaaaaatt aaaagaaata ttagaagaaa tggaggttgg aaatattgag
     1861 taataaaaaa gatgattttg atattacgga tgatatgctt agagaaagta tcccaatagc
     1921 gacaaaaatt attattgatg accttctttc agatgatgaa ttaaatcatg ttttttcaaa
     1981 aggttttgag cgaaaaatga aaaaactcat taagcaacaa aaaagaagta gtcttacaaa
     2041 taaaatcatt ttttattcaa aacaaacagc tattgtattt ttaatagttt tagctagttt
     2101 acttactatt actatgagta gtgaggcatt gcgttcacgt ttctttagat tagtaataag
     2161 agtatatgaa gacttaactt catttgtttt ttcaactaat ggagaagata atcttagttt
     2221 aaaaaataaa gaacctgagt atataccaaa aggatttaaa gaagttgata gagttgatga
     2281 ttttgttatt acatatagaa atgatgaaaa tattgagata caatatgtaa cagataaaat
     2341 tagttcaaat tctattatat tagatacaga aaatgcaaaa gttaaagata tatttgtaaa
     2401 tgggcataaa gcaaaatata tagtaaaagg aactttctta cagttatttt ggaatgacaa
     2461 caactttata tatttattaa atgtagatta tcaaaatagt ggagaatctg aattttatga
     2521 agatattttg ataaaaatag cgaaaagtat aaaataattt ttttaagtgt ccaagggtag
     2581 cctcttcatt cgttatacaa gtatatatga aaatgtgagg tgatttttat ggttttaaaa
     2641 tctacattta cctgagcgtt aaaaatattg ttataggaaa aattaataca ttatagttat
     2701 aacatgcttg caataggaat aaaactttaa aattgaggtg taaaaaatga aatcagattt
     2761 atatttatgt aaaagtaata aattgtatag agacaacgaa gttctagaaa aatttatgga
     2821 agatttaagt tttcttatag acgattataa attctcgtta gaaacaatat ctattctatg
     2881 caaaataaat attgagaaat taactaattt ctatgcagga aaaggatata tactgtatga
     2941 agaaatgtct cttattgaaa gtgtacttgc acctttttta agtgcaattg atattgctaa
     3001 acataattat gatgttgtca ttaaatcgag ggaaagcgat aaacctttga tattcgaaaa
     3061 ttaatttaaa actgggggaa tacacatgaa aagattattt gcgttattac ttactttaag
     3121 tttagttata ttaccatcaa ctttagtatt tgctaatgaa aaagctgaaa atgattcaat
     3181 ttatgctgag gcgccattag tgtttcaaag taatgaaaaa gcatatacac atggtagtgg
     3241 aggaactgca tatataggtt atatgtcaag taaacatctt aattggagga taacatcttc
     3301 aaaaggaata gttatactat tttcaggaac tgttgagatt agaaattcaa aaaatatgct
     3361 tgtagcatca tatccaataa gtggtggagg atcttcaact atatctggac aaatagatgt
     3421 taaatatctt aaaaaagggg attataaagc tatattgaaa ggtacaagta caacaggagg
     3481 cggagtctta gtagtaaaat ctggtctagt tcagatgttt acaatttaat attataagta
     3541 aaataaaaat catgaattta ttgggggata gtcacaatac atattattta cagatattca
     3601 taactattat agttaaagac attctattat aaagaaaatg tataatttaa aattataata
     3661 aaaaaaatta tcattagtgt ccaaaaatca cttcttcatt cgttatataa gttatataaa
     3721 tagaaaacaa atgaagaggt gattttatga atcttagaaa aacagttata agtggtatgt
     3781 gtgctttagt atgttcatca gcagttgcaa tacctgcatt tgctgatgaa tatcaagata
     3841 agaatattga acaatattca gcagtaaact atatagatga tactatggtg aaaatgtctt
     3901 atattaagtc tggtacttgc aatttaagta tttcaggaag tacagctaaa gtaacatgct
     3961 ttgttagagg agcatcaaat gttacaagca catcaattat tgctagatta cagaaaagca
     4021 atgataatgg gaaaacatgg tcaacaatcc aaacatggaa tgcttcagga aatatttcat
     4081 gttctctttc taagtcaaaa actataggaa aaggatatta cagagttcat tccacaataa
     4141 aagctaactc tgaaagtaaa aatataataa gtagtacaaa gaaatattaa aaatagatat
     4201 tgtatttatg agttgtagta aacaagaata atagtttttt aaaagcaaat ataattaatt
     4261 ttaaatagag tgccaatata aaataggtgt ataatattta tactttttta tattggcact
     4321 ctattttata aaataagtta ttatagaaaa tgaaacataa tttatatata aattttctgt
     4381 ttgacctggt atataatctt ttatatcaca tatcagtatt ttcccatcat tataacccca
     4441 accaatcaca gttgcaggta tcatatcagc ttctccatca taaatgattg tatgtttata
     4501 catgattttt ccctcgctat tatctatagt tttattcaaa ataccttctg ctattaattt
     4561 agcaactatg tctttaatag tctgtatctg ctttactgtc tacaaaacat acttcaatta
     4621 atatcgctgg agcttttgta tgactaagcc aatacaattc ccttacgtcc gattttgcac
     4681 ctctattttc agcagattta tatatatctt cagtcaattt agttataatc ttttgattca
     4741 tctaataaaa tctctccctc tgaaactatt tcctgttctt ttttagcctt ttccatttct
     4801 gcatgaggat tatcaataaa acttggttga gataatccgg tttcagtaga taatttatct
     4861 ccaagttgac ttaatatatt tgctacaact aaatcattat gtggtatctt aggggtaaat
     4921 ttagccttaa tatctttata atcatagtca aaaccatatt ttttattaag ccaaatgcac
     4981 aataatttgt acctattaaa taaagtatct gcaatactat cattattctg gctacaaatc
     5041 cattctgtac tgattaatct tgctacaact gctaaacttg atgtattact ttgcattttt
     5101 tcattgtgat ttatatgaga tgtaatttta tatatgtttt cttttattga agatagtgta
     5161 ttttgaacaa atgtatcatt catgtcttta ataagccatt ctatttttcc atctccatta
     5221 acttgaagta ttccaagttc tttcatcctt ggcaaatcat cttctttaat attacattca
     5281 gagaaggtta aataagcatt tctaaaatca ctaatttcat tacttatatc acttaaatta
     5341 gtttcataag catcttgtaa tccttttata tcattgaata ttgtattttt tcctagttct
     5401 tcacttcttt tacaaattga tattggaaca ttaccattaa atatattatt tactgtaggt
     5461 ttttctatct ctttaaaatt tgaatcaaaa tgatatatgt attctttatc atatacatct
     5521 atataaatat catctttaaa atctttttta aattctctaa aaaaatagat ggtctctcca
     5581 tcttcatttt cataatggca gccatctgtt ggttttatta ttcttacttt catttcattg
     5641 ttttttacat agtaaagttc atagcaaaat ccaaataata gtgtatatct caataaatct
     5701 gaatcgtgct ttttactcca atggcaagta tagtattcta aatcatttat aatcttctca
     5761 tcacctaatc tacttgtata ggtgatttta tttgcaagag aataagcgac ctcttcatta
     5821 ataaactttt ttaagaaatt agtgtttatc ttattattag acctcttggt aaccatttta
     5881 tagttactaa tagcatctgt ttcaccttta taatacttat acatagtgtt gtattcttgc
     5941 ttttttaaac tctagtcttc atatatttct cttaataaac ttaaatctat ttccatttaa
     6001 tgcatcacct ccatacaatc aaagacctaa atttcttcta tctaatagtg taatactttc
     6061 atttacttta atattaaaaa tcctatttat aaattctgct gatatatctg gtgcatcatc
     6121 atgtagagag tacttttgtc ctgtaaaatt aaggatttgc tgtataaatg ccttatcctc
     6181 ttctgcaaaa atcatttgac ctttatttaa aacaggtatt aaagtagaga ttttatcatc
     6241 tttatttttc ttttggtgct cattaatgat ttctatatct ctgtagtaaa gaacatcatc
     6301 attttttatt ttaagttcta attgattagc agctgtacca ttaaatgtat ttttttctat
     6361 ataaacatgt gttatatctg gatacacttt taataaataa atcatatgca atatatattt
     6421 atcaaaatca gttctagcat ttattttggc tagttctgcc aatctgccac atagtaaacc
     6481 attttctgat tcacttccaa caagaaaagc actatagtca tgtttagaac cacctgcaga
     6541 tgctgggtca cataataaca ttgtcttttt gaatgtatga gtttctatac attctctaga
     6601 ttaagttctg attgttttaa accatttctc acctattgaa tttacatcat tttgtacctc
     6661 ttgtttaaaa gatgttgggt tttcgtaata acttaaagcc atgtctaaac aattccaaaa
     6721 acttggccat aatataggaa atttaatttc tttttcattt tcccagtaaa attctttagc
     6781 atcttcaaga tgatttgtgt ttttgaaatc aaataacaac tctttgaaat taagccataa
     6841 accagaatta aataattcat ctacattatc aactaaaact cctttttcac atttaaattt
     6901 ccaagttggt aaattcttta gcctactata gaaacattct ttatgttgta aggttcctaa
     6961 tgctataaaa gttgttcctc tttttataat cttaccattt cttattactg gtttttaagc
     7021 agcatatttt acatcatcag aaaatttttt aaattttttc tctctagcat cttctgttct
     7081 aacattttct tctgactgat aatcatcaag tatgataaga tctggacgat tattattgta
     7141 tttttttcct ctcattggtg atgaagatga tattgcttca ataaaggttt tattagttaa
     7201 ttctaattga gtactattgc aaatatattt tctatcatta tcatttaaga gaactccaaa
     7261 tgctttttta atatactcat tttcaagtaa tgcatttttt atatctttta cgaatttttc
     7321 tgctgtagag cctatatcag agcaaattaa agtatatgtc ttatgtttat aacaatgtga
     7381 ccatacagtt gttgctaaat ctccaaatgc acttttacct gttcctcttg gataaattct
     7441 tcctaattgc tgtgaaccat ctccaattat agactcttgt atatcttccc ataactcatg
     7501 atgaacttta gctataggag cagcaacatt atcttctttt ggtaaatatg tgtcttgtat
     7561 aaaatacata cagaaaaatt ctaaatttat ttttccaagc tgataagcta ggccattaac
     7621 tccccaaaga ttgcttgaat tagtttttat aatctcattt gcttcttttt cagcttctct
     7681 tatagttgct ccatttctta taaaggtctt tgccatataa ctaaatatta attgtatatt
     7741 tctttctaaa ttatccactc gtatttactt cctcttaatt cttctatttt tttaggttca
     7801 gtgagtaata caattaataa aataaacaat gcaaattttt ttctaataaa atcaccctct
     7861 ttgaaatatg tctctggttt ttaatttttg ctagaaaatt tctagaactc attttctata
     7921 aataatactt ttttgaaata gaaggatacc ccctatactg aaaaacagtt atttataatt
     7981 attttaaaca taattaaagg gcataattct atgaatttgc cccactaaaa ttaacttaag
     8041 catatatatt gctcttctta gttactctca tggctcttta ttaatctatc taatgttggt
     8101 ctacttactc ccaattcttt agccaattga gtcttattaa gttctctagt tttatatcta
     8161 tcataataac taccaaagtt gtctatctta acttctttac gtcctttata tttaccctgt
     8221 tctttagcta tagagatacc ttctcgttgt ctttctaaca tgtttgttct ctcaaattca
     8281 tatattgcgc caagcattgt aagcataagt tttcctgtag gtgtacttgt atctaaattt
     8341 tctttatcac taattaactt tacatgtttc ttttctaaga gctcaactat ttctaataag
     8401 tctttagtac ttctagcaag tctactaaag tcatgtacaa ttattgtatc tccttctctt
     8461 acaaaatcta acattgcgtt tagttttgat cttttagtat ctttggcact tattttctcc
     8521 tgatacattt tttctacatt atgctttttc attgtaacta attgtctatc ttcattttgt
     8581 tctgctgttg aaactctcat ataacctact accatctttg atacctccac ctatgtttac
     8641 tctataaata atattatagc ataaatgtaa aactagagtt aaagatttgc catgcttttt
     8701 gtaaattaat tggagttttg attttatttt tacatcagaa tagatagttt gtaatgtaaa
     8761 tttgtaccag catactttac aatatgctct taggaaagat atttcttgtt tatagattat
     8821 aatatttctc tagtatcttt atttttataa tttcaaaaat aaaaattaaa attacagtta
     8881 ttatcatatt tcgtgtacta ttttctataa tattataact aaacaatgca aaaacaatga
     8941 ttaataaaat tacgtttata gtgccagaaa tcaagaatga ttttttctta ttattcatat
     9001 atttcctcct agttatttgc tagttttata ccatctccta ctctttcagt atatacaact
     9061 tttttaaagt tagatgagct ataaaaatat gtaacatatt catatctgta tggaacagga
     9121 acattagcat gactgtttct aatatcagtt atttgtctcc tatagtatgt ttttgtatat
     9181 acgcctccat atttagtaat gtcatatact ttttctctat aaatactctc agctaaacct
     9241 aataaaaaag atgaccctcc agaaagataa cctaatcccc attgaatagt ttgtataatt
     9301 ctttctcttg tataaggaga acttaatttg cgtgattttt gacctagata tttaacattt
     9361 ttccaactac caggtgtaac ttttgttgaa acataagtgt tattgccttt tttatccaca
     9421 atcatttcat tttcattgct attaataata gtaaattcgt tttcattaac aagttcttgt
     9481 ttatattcat tttcttcaaa agtagatgca taaaccaaat tatatccact agataaaaca
     9541 aatattcctg ccattaaaaa acttattatt tttttcttta acataatatt ttactacctt
     9601 tacttaaata tttattctat aaacactgtc atataacctg ttagttatat taaaaataat
     9661 ctttttatat aacttcacaa tcaaaccttg tcttatttca taatagtttt atttaatatc
     9721 atataattta gacctcctta taagattatt tatatatata acgaataata aaagtaattt
     9781 tggacaaaat aaaaatcttt ttattttaag aatgaaaaat ttaaccctct aagaaaactc
     9841 aattaagagc gattcataga gggcttatta ttatattata taactttata tactttttat
     9901 tttcatatgc ttcaatttaa cttatttcaa gcatattcaa gtacttctca ttaactttat
     9961 atacatcctt ttctctagat aaaatctcta tttcttcaag attattaaac acttccatca
    10021 actggtcaac taattcgatt tcttttattc cagcctttaa gtatctacct ttattctttt
    10081 tgatatagtt ctctaatgtc atagaataat tatctttact acagatatat tttaatatat
    10141 gttcttctgt tgctgataga gttatatcat tttcaaataa ttcctctttt aatatactct
    10201 ctaaagactt cctatcatgc atatcacgca tttcattgat agattgtatt atagacttta
    10261 gcatgaactc tataaataca gttaaattat atcctttatt ctctacatct agtatagcct
    10321 tataatactt agttctgttt ttagatatta tatatgatat tgaaaactct ttaaaagtat
    10381 catatccttt attaattaaa tatagatatg ataaggctct tgatgttctt ccattaccat
    10441 cactaaaagg atgaacatat acaaagtaga aatgaattat tgtactcttt actaaatcat
    10501 ttactttact attttccata aaaattatta attcatccat aaaattatat attttcaaag
    10561 gatcaagtcc tgtatgtata atctcacctt ttgaatcaca tacatatact ttttcagttc
    10621 tatattcata ttcttctgaa tctaaacagt tttcacccaa tatatgatgt aaattatata
    10681 taaatttgtg actatataat ttatctaaat tatctaatcc atattctaag gctctatgat
    10741 tattgtaaat catatactca cttttattac ttggtttcat ctttcctcta attatagatt
    10801 ttgcaatctt tctagttgaa aaggcacctt ctattacact agaataataa gactctaagg
    10861 ctaaatcatt tttatcttct attattttaa atatcttgct tgatgttaat ctatctattt
    10921 catcttttga atttaatatg ttatcagttt catgatagta tagttcttta ttatcaaaac
    10981 tctttaagga aagtataatt ttatctccag tagagtattt atttttgtat tcttctattg
    11041 tcagtttaat caccacctta gtttatcttt atctattaat attaacaaaa agtattagtt
    11101 tttacactat ttttgattgt ttattttata tattatgatt ctactcttga taattaaatc
    11161 ttacttaaaa attaattctt gataattaat aaaaaaattt atatataagt gtctaaaaaa
    11221 cgattcttta ttcgttatat atataaataa tactagaaag ggaggattat attatgatat
    11281 ttagtaaacg ctcttttaga aaaaaacggt gataaacttg ttttaaattt tagtagaata
    11341 tgatttttgt tttggcaagt attaatacat atattaaaga gggaggttta atatgttaaa
    11401 taaaaaaaat ataagttcaa ttattatgac aatgatttta agcttatcaa ctactgtatt
    11461 tgttttcgct gatgaaaata ctaatgtgaa aggacaagat gtaaccaaat ataatgaaga
    11521 aatggtctct aaaaacttag aagatttaag tatagaacaa ttaatagata ttagagataa
    11581 agctattgat aaaaatttag aaggtcttag aaaatttgga gaagattcag tgaaagaacg
    11641 taaagattca attttaaact ttgaatttgg attagtagct tttgttaaca aaaattatag
    11701 taacttaaca gaaaaagaga aattaataag acaaatcaat attgttgagt atgaaaattc
    11761 tataaatgat ataatggatc cttcaggcga ttttttcaaa gtaacaaacc aaagttatca
    11821 gattaatgaa tttgtattaa aaaatggaaa aaactataaa gagtggtcgt atgaatatgt
    11881 agagtttttg attgagcatt tagaggattt atgtaatttt gtagattttg atgcaaaata
    11941 tgtaatagaa atggttgatc cttcaataaa accagatctt tcagataatc aaaagaaatc
    12001 tctaaataat gaattaataa aaatgtattc aagtaatact ctaagtacaa atataaaaga
    12061 tgaaatagaa aactgggaaa ataatttaaa tattaatgag aaaaattcaa atgaaccttt
    12121 accaagagta agcccttcta gttatagaca acaagcatta gcttatgcta aaaaacatgg
    12181 ctatactaat ggttattatg gtagtacaaa tagaaaaaat aatgcacctt ctccatatta
    12241 taattttcaa aaagatggat atggagattg tgctaacttt gtttcacaat gcttacatga
    12301 agctggagta acattctgga cgaataataa tccatggtat tattattcta ctagtaatag
    12361 atcaccttct tgggctggag caagagaatt taaaatacat tggatgaatc gaattgcata
    12421 tcaaacactt actgtatcaa actctttaag ttttttaaga ccagcaacac ctgtaagtat
    12481 attaaatagt agtggagtag caactcatac actaatatct acagataagc attcaaatgg
    12541 atataatttt tcatatgctg cacattcaga tgaaggtgca agaacaaatt tgttaaaaaa
    12601 acttgaagga aagaaaattt cttattacaa agtttattgg gaataaataa aaatttatac
    12661 ttttaattaa atgaaaattg aaaagaataa atatctattt caagatccaa taagtattta
    12721 gttgaagcag ctattaaatt ttattacata atcataaaaa agagactagt ggaatttagt
    12781 ctctttttta tgatttaaaa atttaataaa agaattatat aaatttgtct aaaaattagt
    12841 ttatcattcg ttataattat ataaactata tcaaaaaagt gttctattgt attctttaga
    12901 ataatcagta ataaaggtta ttctaataaa ctgagttatt ttttcacaaa taaaatactt
    12961 attaaaaagg agatttgata tgttaaacaa aaaaaatgta agtttaattg ttttagtaat
    13021 agttttaagt ttattcactg ctatatttgt tttcaataat agaaatacaa atgataaatc
    13081 aaaagaaatt gctaaaaata atgaagaaat agtgcctaaa aacttagaag ctttaagtat
    13141 agaacaatta acaaaaatta gagataaggc tattgataaa aatttagaag gtcttagaga
    13201 atttggagaa gattcagtaa aacagcgtaa agattcaatt ttaaattttg aatttggatt
    13261 agttgctttt gttaacaaaa attatagtaa cttaacagaa aaagagaaat taataagaca
    13321 aattaatatt gttaactatg aaaactctat aaatgacata atggatcctt caggttcatt
    13381 ctttgaatta gcaaatcaaa gttatgaaat taatgagttc atgttaaaaa acaaaaaaaa
    13441 ttataaagaa tggtcatatg aatatataga attcttaatt gatcatttag atgaattatg
    13501 caaatttgtt gattttgata taaaagatgt aatagatatt attgatccta caataaaaac
    13561 agatctttca gaagaacaaa agaaatcttt gaatgataaa ttaaagaaag tgtcttcaag
    13621 tgatacttta agtgaagaca ttaagaagga gataaaaaat tgggaaaaca atttgaatag
    13681 tttaaatatg aataaaaata attctaatta actgatagtg taaaatgtac ataattataa
    13741 ataaaatagg gatgtcttaa aattgattat tttctaatct aaatatttta ggcctatcta
    13801 aaaacataag attatggtct aaaatttgaa attttagacc atgctttatc tttacaataa
    13861 atgggtcttt aaaataaagt gtgtattctt ctaaaaagtg gtgataatta aaatattaaa
    13921 tatcactata taaaggaagg tgcacacttt ttatgtttaa caaaaacgaa atattaaaaa
    13981 aacttattga agaatatgat gttaaaacaa ctacagatat tcaagatatg ctaaaagatt
    14041 tatttgcgag tacaattcat caaatgttag aagcggagct tgacgaccat ttaggttatg
    14101 atagatatgg caataagaat aaaaatacta aaaactctag aaatggatat agaaataaaa
    14161 atgttaaatc agattttgga gaaataactt taaatattcc aagagaccga aatggagatt
    14221 ttgaaccaaa ggtaattagt aattataaaa atgatatatc aggaatagag aaccaagtga
    14281 ttggaatgta ctcaaaaggc atgtctactc gagatatatc aagccacctt aagagtattt
    14341 acggaattga tatatctcat tctctagtag ctaaaattac tgatagagtt ctacctttag
    14401 ctcatgaatg gcaaaacaga cctcttgata tggtttatcc aattgtattt atggatgcta
    14461 tacattataa agttagaagt gaaggtagaa taataaacaa agctgcatat atagccatag
    14521 gtgttaactt agaaggtata a
//
LOCUS       DAEPOZ010000093        14470 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.093, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000093 DAEPOZ010000000
VERSION     DAEPOZ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14470
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.093"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            949..2307
                     /locus_tag="KR440_002017"
     CDS             949..2307
                     /locus_tag="KR440_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBH0699066.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     ASGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            2413..3207
                     /locus_tag="KR440_002018"
     CDS             2413..3207
                     /locus_tag="KR440_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBH0699067.1"
                     /translation="MVTIKEYSIPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDEKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            3200..5506
                     /locus_tag="KR440_002019"
     CDS             3200..5506
                     /locus_tag="KR440_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBH0699068.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVAESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRIIRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            5645..6922
                     /locus_tag="KR440_002020"
     CDS             5645..6922
                     /locus_tag="KR440_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH0699069.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIESLKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            7105..7827
                     /gene="yqeC"
                     /locus_tag="KR440_002021"
     CDS             7105..7827
                     /gene="yqeC"
                     /locus_tag="KR440_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HBH0699070.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSFINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHKTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKNIVLNPNGLFKNYSGKRVLFINKVENEKYKNLAIKLIENI
                     KEYESEIEIFYGSVKQKFCVRY"
     gene            7934..8503
                     /gene="mocA"
                     /locus_tag="KR440_002022"
     CDS             7934..8503
                     /gene="mocA"
                     /locus_tag="KR440_002022"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HBH0699071.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
     gene            8500..9519
                     /locus_tag="KR440_002023"
     CDS             8500..9519
                     /locus_tag="KR440_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HBH0699072.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
     gene            9857..11482
                     /locus_tag="KR440_002024"
     CDS             9857..11482
                     /locus_tag="KR440_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0699073.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALFNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
     gene            11681..12157
                     /locus_tag="KR440_002025"
     CDS             11681..12157
                     /locus_tag="KR440_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699074.1"
                     /translation="MVKKRLVAVVLSLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQPNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(12365..13477)
                     /locus_tag="KR440_002026"
     CDS             complement(12365..13477)
                     /locus_tag="KR440_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBH0699075.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            13818..14195
                     /locus_tag="KR440_002027"
     CDS             13818..14195
                     /locus_tag="KR440_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH0699076.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
BASE COUNT         5660 a         1500 c         2562 g         4748 t
ORIGIN      
        1 tatattttat gttgcagata caaatatagt aaaggagagt acattggtac aactgagata
       61 catagcttat taagtttttc tgtcttatga gaattatatt ataaaatatt cataaatatt
      121 ttgagtttaa attaaataat tattttatgc ttagattaat tgatatttaa ttaatttaat
      181 aacaaaaaca gtgaaagagt aaataatatt atttacaggg ggtaaagcaa agtatgttaa
      241 ataaattgta cttttggccc cgtacataga gaaatctatg taggacacaa ctttaaaacc
      301 agtaaatcct aaagccttgc aactacaata aaagatagaa agaaactttt atgaatgtgg
      361 tgaaaaccag aaacaatagt gaggatggta tatggtgaga gcctaagtac ttaaaaatgg
      421 acatttggtc gccaagccta gaaataggaa ggtcaaacga ctatctcgag agagagtaca
      481 ctataagcga ttgatagtgg aaacgggttg ctcttagcag gtaatgctga agatgaagaa
      541 atagtctacg ctcatatgaa agtatgaggt cactagattt gaagtgacat ctataaagta
      601 gcgtttatag gtaaatgttt aaaacttgaa acatgtaaat acttatgaaa acttataact
      661 tataatgatt aagattgaaa gatttaaatt gaattaaatt tactataaaa aattaaaaat
      721 tcatagctat aatactctta tatttgtaaa gcatggtata taatgtatac aaaattaaag
      781 aatacgtgta tttgttaata attaaattta ttgcatctgt tttttaagac taaaaactta
      841 aatatatgtt ggagtatagt tgatatttat gaacatcatt aatttaatat attagatttt
      901 taagtattta tataattcaa taagttttat gtaacgggag tttttattat gaaagcaaaa
      961 agtacatcaa aatttgaatt agacggaata ccaccactaa aggaagcaat acctttaggg
     1021 ttacaacact taatggcaat ggtaataggt agttgtttac cagcgatatt gattgcaaat
     1081 gcatctggac ttaatcataa gatgtcaaca ttattgattc aagccgcatt agtttttgca
     1141 ggattatcaa ctttattaca attatatcca atacctttgt ttaaaggttt taaattagga
     1201 ggaggaatac cagttataat gggagctaca gctatgttta taggagctgg ggtatctgtt
     1261 gcaagtaagt atggactacc agtattattt ggctctcaaa taatagctgg tatagtaata
     1321 attataattg gttttggtct taaaaaaata cgatttatat ttactccagc tgtatcagga
     1381 acaatagttg catgtatggg tcttggactt tttccaatag ctataaaaaa cttagctggg
     1441 ggtatgggca atgagacttt tgggaatcca attaatttta tagttggaat atctgtatct
     1501 cttatgatat taggattaaa taagtatgga aaaggattat ttaaagatgc atcaatatta
     1561 gttagcataa tttttggata cattttatca ttaattttgg gtatagtaaa tttttcttca
     1621 attcaggagt ttactttggt tgcgcttcca aaaccacttg cttttgggct agatataaga
     1681 cttgaagttg ttgtaatgtt ttcaataata tatttagttg agatagctga tataatgggt
     1741 gcttgtacac tttctgcggt aggagggctt aatagacaag taacagatga agaactttct
     1801 tctgctgttt taggaaatgg agtactctct acaataggtg cattattttc tgcaactcca
     1861 atggggatgt ttggacaaaa ttctgcaatc gtttccaata caaaggttgt aagtaaattt
     1921 gtactagcta ttggaggaat tggattattt ttggcaggaa tatcgccttt attagctaat
     1981 ttaataagaa caattcctcc ctgtgtggtt ggaggagcaa cactcgttat attttcaact
     2041 cttacaacat ctggtttaag acttgttagt atggatggat ttaatcaaga aaatagcatg
     2101 atattaggtc tttctatggc ttctggtatt ggattcatgg tagctcctca agttcttgaa
     2161 aaatttccaa aatttataga gacattactt gcagattcaa gtgtagtttc aggagcaatg
     2221 gtagccataa taattcaagc tttatatatg gtaaaatttc ctggaaataa aagtgataag
     2281 gttgaggata aaaataaaag tttataatgt aaaggtttaa tatatgtgaa ttaaatatac
     2341 tacaattata aatataaata aaagatttat taaaaacttt tgtgttttaa taagttaatg
     2401 atgggggata agatggttac tataaaggaa tattcaattc cacagagttt agaagaagca
     2461 tataaaattt taatttctaa aaaaaataat gttattttag gtggatgtgg atttataaaa
     2521 ttaagtaata aaaatatagg aacagcgata gatttaaaag atataaatct aaattatata
     2581 aaagaggatg aaaaaaatat tttgataggt gcagatactt cacttagaag tttagaaatt
     2641 gataaaacta taaaaaatta ctgtagtgga atactttcaa gcgctgtttc taatatagtt
     2701 ggtgttcaat ttagacaagg ggctagaatt ggtgcaagtg tattttcaaa gtatggcttt
     2761 tctgacttaa taccagcgct tttggttgtt ggagcgaagg tgagattata caataaagga
     2821 atactagagt taagtgagtt tttggatagt gaattaagta gggatatatt ggtcgaagta
     2881 atactaccaa aaaaagatgc gatagctgtt tttgactcaa taagaaaatg cacaggagat
     2941 ttttctgtat taaattcagc aatgctaaaa gaaaatgata cttacaaaat agcaattggg
     3001 gcaagacctc aaaaagctag attagcactt gaagcgagta atatattaaa taaagaaaaa
     3061 gatatagata aggcatctat tgttgctagt aaggaactta cttatggaag taatatgaga
     3121 gctagtaaag agtacagaaa ggatatggca agtgcattag ttaaaaggat gtataatgct
     3181 attgaggagg gaatgtataa tgattaataa ggggataaga aagatagata gtacagcaat
     3241 tgtcacagga aaacctctat atactgaaga cctcattatg cataaagatg ttttgacatt
     3301 aaaactactt agaagtccac atgctcatgc taagataaag aatatagata catcaaaagc
     3361 cgaaaaaatt ccaggggttg tgggtattta tacgtataaa gatgtaccac aggtcagata
     3421 ttgtgtgcca ggtcaaacag aaccagaagc atcaccttat gatagagttt tacttgaaga
     3481 tacagttaga tttattggag atgaagtagc tttagtggct gctgaagatg agaaaacagc
     3541 acttaaagcc ttaaaaatga taaaggtaga ttatgaggtt ttaaagcctg tgtttgatgc
     3601 aagagaagca gaaggaaatg aagttgttat acatccagaa aaagacatag tagagccata
     3661 cccttttgga ttggatgcaa aaaataatat agtatcaaaa gaattttttg aatttggaga
     3721 tgttgaagct aagcttaaag aaagtgattt tgtggctgaa tcttcataca aaacccaaag
     3781 tcaatcgcat tgcatgatgg aaactcatag gtcatattca tataaagata taaatgatag
     3841 aattgttgta gtttcatcat tacaatcacc acacaatgta aagagaataa ttagaaaagt
     3901 attgggtata ccatcttcaa aaataagagt aattaaacct agaataggtg gtggatttgg
     3961 aggaaagaat atagcagcaa cagatatatt tactgcattt gttactatga aaactggtag
     4021 accagcaaaa tttgtattta caagaagtga aacttttgta ggttctaata gtagacatga
     4081 aatgtttttt gatgttaaag ttggtgcaga taaagaagga aatttaaaag caatagagct
     4141 aaagtcatta aacaatacag gtgcttatgg tgggaatggt gtctctgttt cttccgaatc
     4201 tggacacaat accttaccaa tatataatga tattgatgct gttagattta atgctagaac
     4261 agtatatact aataggttac cagcaggagc acttagaggg tatggtgcta cacagggaat
     4321 atttgcaata gatagtgcta ttgatgaatt ggcacataag attggaatgg acccacttga
     4381 atttagaatt aaaaatataa tagataggta taccaatgga aaagcagctg aagacgaaat
     4441 attaagttgt gagctgagac aatgtataga aaagggcaag aaattaatca attgggatga
     4501 taaatatcca tgtaaagata taggaaacaa caaagtaaga gcagtaggta tggcagtagc
     4561 aaatcatggt tctggtatac cgcgtgtaga tatggcaaca gtcactataa gaatggatga
     4621 agatggcaca tataaactat tatctggtct tgctgactta ggtcaagggg ctgatacaat
     4681 tcaagctcaa attgctgcgg aagctttaaa tactactgta gatagaattt ctatttatac
     4741 aggtgattca gatttatgtc cctatgatac aggagcattt gcttcttgta caacaacggt
     4801 tacaggaaat gcaacaataa agacagcaaa taaattaaaa gatatattac tttcagttgc
     4861 agagtcaaag ctaggattta aaaaagaaga tttagagttg ggaaaagaca gggtttttgt
     4921 taaaaacgat aatagcaagt ttgttctatt agaggaattg ggtagagaat ctgttggagg
     4981 aataggattt gctagtgatg atgtaccagt agttttggaa gcaacagaat cttttggcat
     5041 gttgaaaaaa ataaaaccat ttatagcagg atttgcagag atagaattag ataaaaatac
     5101 aggcgaatat aaggtcataa attttgcatg tgtacctgat tgtggaacag ttataaatcc
     5161 aactttagct agaatacaag tagaaggtgg tgtgatgatg ggtatagggt ttgcaatgta
     5221 tgaagaccct atatttgata aagatgggag attattaaca gatagtttcc tacagtataa
     5281 agttcctaca aaaaaagaag ttggaaatat attggtagat tttgctgaga atttcgaccc
     5341 aggaggtcct tatggtgcta aatcattagg agaaatagtt atacatacac cagcgccagc
     5401 tatagcaaat gcaatattta atgctacagg agttcgttta agagagcttc ctatgacttt
     5461 tgaaaaagtt tatatgggta tgaaaaataa taataaacaa ttataataaa taataaggtg
     5521 tataaaagca agtaaattta aattattttg taaaataaag taattaaatg gacattatat
     5581 atggtgtttt gtaaaaatac cgtatataat gtctagtaga gtatattcta aaggaggtga
     5641 gttcatgtca aacagaataa aaaatataca ctatggaact cagagacaac aattaaaaaa
     5701 cttaactaaa aaagaatatc aagctctacg agagatgtgc ttcttagcta agaacttata
     5761 taatgttggt acatataatg taagacagca ttatttcaat caaggagaac atcttaacta
     5821 tgaagctaat tatcatgtat gcaaagaaaa tgagaactat aagcttctca atagtaatat
     5881 agcgcaacaa atattaaaag aagtagacag ttcgtttagg tcattttttg ggttaattaa
     5941 attagccaaa gaagggaaat atgactttag agggataaaa tcacctaaat atttagataa
     6001 agaaggattt tttagcataa taataggtca aataagaatc aaagaagatg ggattttaaa
     6061 tgtacctatg tctccagcat ttaaaaaact atatgggaaa gtaagtataa aagttccaca
     6121 aaatatacta gagaaaaaaa ttaaagaaat aagaataata cctaaacata aagctaggtt
     6181 ctttgaaatt cagtattgtt atgaaatacc gaaatcagag gaagtttcaa atcaaaaaaa
     6241 tgcactagct atagacttgg gtttagaaaa tttatgtaca tgtgttacca atttaggtga
     6301 tagttttatc atagatggaa aaaggctaaa atctgtgaat cagtgggcaa acaaacagaa
     6361 tgataaaata gaatccttaa aattaaaaaa gaatataact gcaataacaa gaaaacaatt
     6421 taaagtttgg aataagagaa ataatcaagt taaagactat gtaaataaaa cctgttttta
     6481 tataattgat tattgtgtaa aaaataatat tggaaattta attgtaggat ataatgctaa
     6541 gttagaaaaa agtgataata tggagagaaa aatatataga aattttataa gaatcccatt
     6601 tggagagata aaaggaaaat tagaatatct atgtaaaata aaaaatatca attttattag
     6661 gcaagaagaa agctatacta gtaagtctga tttttttgct gatgattttc tacctgatat
     6721 caatgtggac aatgttaaaa aatatgactt taaaggggaa agaataacac gtggacagta
     6781 caaatccagt gttggaatca ttataaatgc agatgtaaat gcgagtctta atatactaaa
     6841 aaaatcacaa gtagttaatt tatctaattt acagaataat cctcaaatat taaagcaacc
     6901 acagagagta agaatatctt aattatattt aaaagataaa ctgatagcgt aagcttacag
     6961 cttagcggcg aattggatcc gccttgttgt ttacaacaga atctatttac tttcgagttt
     7021 atggaatttc aaaaatcact tttactaaaa aggtctaact ataataatta agagattaga
     7081 aaactggtta taagaggtga aagtgtggaa ctatcaaata taatagaaaa gaatgaaata
     7141 ataactgtag ttggagcagg aggaaaaact tcgtttataa actattttgc aaatttttat
     7201 agagataaat tgaaagttct tcttacaaca acaactaaaa tatatgtacc aaacgattat
     7261 gacaatataa ttattacaat agatggaact gttattccat ctatttgtca tggaataaca
     7321 gtctgtggca gttatataaa taatgaaaat aaattagtaa gtattgattc ctctattttg
     7381 gatgaaattg tagaccaatt tgatttagtt ttaatagagg gagatggctc aaagagaaaa
     7441 aaacttaaag gttggaatgc taaagaacca gtagtatatc ataaaactac aaaaaccata
     7501 gggatattag atataacatc atttggaatg aatattaatg aagagaatat acatagagta
     7561 gaaatattta aaaaaatagc aaatttagat acaagtagta ttaatagtag ttctacagta
     7621 agtattgaaa acttaaaaaa tatagtatta aatccaaatg gattatttaa aaattattct
     7681 ggaaaaagag tattatttat taataaagtt gaaaatgaaa aatataaaaa tttagctata
     7741 aaattaatag aaaatattaa agaatatgaa agtgaaatag agatatttta tggaagcgta
     7801 aaacaaaaat tttgtgtaag atattgaagt attaaaatag tttatatgat attatatata
     7861 tagataatat caatagagat attataaaaa tcatagaact atcaatatat tataaatacc
     7921 aaggatgata tatatgataa atgcagtgat aatggcatca ggctttgcca gaagaatggg
     7981 agcaaataaa cttttattaa attataaagg ctgttctata atagaacatg tatttagaga
     8041 agttagcaag attgattttc atcaagttgt agtagtaagt caatatgaag aggttttgtt
     8101 attgtgtaat aaatatggtt ttgaatatat agataataaa aatgcaaata taggtcaaag
     8161 tgaatctata aaattaggag ttttaaatag ctcaaaatgt gatggttata tgttctttgt
     8221 aggagaccag ccatttattg ataacttata tatagataaa attataaata cttttaaatt
     8281 agatagagat tttatagtaa ttccaagata caaagatatg tgtggaaatc cagtcatatt
     8341 cccatatagt aagaaagaag aattgttggc acttaaagaa gatgaaaaag gtaaaaccat
     8401 tttaaaaaat tcatcaaaaa taaagtatgt agaagttcct aaagaaatgt tatttgatat
     8461 cgatacaaaa atggactatg agagaatcgg gggtaaatta tgaagctaat aaaaactata
     8521 gatgctgttg gacaagtttt atgtcacgac ataactcaaa taatacctgg agaatttaaa
     8581 ggaagaaaat ttaaaaaggg tcacatagta aaagaagaag atattccagt gttattatct
     8641 cttggtaaag ataatttgta tgtatgggaa aagtcagaag gaatggttca tgaaaatgaa
     8701 ggcgcattgt ttttaaaaga actaactgct ggagaaaatt tagattttag tgaaataaaa
     8761 gaaggaaaaa tagattttat agcagcatgt gatggtctat taaagataga tgttgatgca
     8821 ctttttgatt tgaattgtgt tggagaaata atgatggcta ctcttcataa taattttata
     8881 gtaaataaag gtttaaaagt agctggaaca agagtgatac ctttggttat agatgagaaa
     8941 aaattagaag atgcaaaaaa agtagttggt aatagaaaaa tagtaaatgt agtacctttt
     9001 aaacctaaga aagtaggaat tgttactaca ggaaatgaag tgttttatag cagaattgtt
     9061 gataaatttg gaccagtaat agaagaaaaa gtaaaaggct ttggttgtga agttatagga
     9121 cagactatat gcccagatga taaagaaatt ataaagagtg caataaaaga atttataagt
     9181 caaggagcag aacttatctg ttgtactggc gggatgagtg tagacccaga tgatgttacg
     9241 ccaacagcta taaaagaaac tggagctgat ttagtgacat atggttcacc aatattacca
     9301 ggagctatgt ttttacttgc ttattatgga gaagttccaa ttatgggtat accaggatgc
     9361 gcaatgtatc ataaaactac agtatttgat atagttttat ctagggtttt aattgatgaa
     9421 aagcttgata agtatgatat agctagatat ggtcatggtg gcctttgtat gaactgtgat
     9481 gtatgtactt atcctgcatg taattttgct aaaatataaa gttattaaaa aagagtattt
     9541 acaaattaag tactcttttt aaatatgaaa caaaacagct agaatgatgt tttaagaaaa
     9601 gcatatttat ttagaaatta taataactta ttttcaatgt gtaaaagaat gaataaagtt
     9661 gcaatttgat ttctttataa gtataatata agagattgtt aatttttata aatttacaat
     9721 ttttagtatt attttaataa atatttcaca aatttagtat aaaaaatata ctatttacgt
     9781 gataatatat aataaaaatg ttatcaaagt tttatgataa aataaattat gagcaaaaag
     9841 aaaggaaaat atattaatgt tacaagttac aggtgttgga cttagatttg gtgataagga
     9901 actatttaaa gatgttaact taaaattcac taaaggtaat tgctatggaa taataggagc
     9961 taatggtgcg ggaaaatcta cttttttaaa aatattatca ggagaaatag agcctaatac
    10021 aggtagtata tctataactg ataaagaaag aatgtcagtt ttaaaacagg accactttga
    10081 atatgaagaa gaaactgttt taaatgttgt tataagaggt catgagagac tttggaacat
    10141 aatgaaagaa aaagatgcat tgtatatgaa ggaagacttt aatgaagaag atgggataaa
    10201 agctgccgaa cttgaaggtg agtttgcaga gcttgacgga tgggatgcag aaactaatgc
    10261 tgagaaaata cttatgggtc ttggcataac gaaagatatg cattacaaac aaatgaagga
    10321 actagttggt ggggaaaagg ttaaggtatt gttagcacaa tcactttttg gtaagcctga
    10381 aatactatta atggacgaac ctacaaacca cttagatttt aaatcaataa actggttaaa
    10441 taattttata atggatttag aagagtctat agttataata gtatcacatg atagacattt
    10501 cctaaatcaa atatgtacta atatagttga cgtagatttt ggtaaaatac aaatgtacgt
    10561 tggaaactat gatttctggt atgagtcaag tcaattggct ttacaactgg ctaaggacca
    10621 aaataaaaag actgaagaaa agatagctca attaaaggaa tttatagcta gatttagttc
    10681 taatgcttca aaggctaaac aagcaacatc aagaaagaaa cagttagaga aattagaagt
    10741 tgaagatata caaccatcaa gaagaagata tccatatgta ggatttacac ctgcaagaga
    10801 aattgggaat gaagttttag aagtacacaa tttaactaaa actatagatg gggttaaggt
    10861 tcttgataat gtgtcattta gattggatag agatgataaa gtagtattta tgggagatga
    10921 aatagctact actgcacttt tcaacatagt tatgggagaa cttgaaccag acagtggaga
    10981 gtataaatgg ggagtaacta cttctcaaga ttatcttcca aaaaatcata ataagttttt
    11041 tgacggagta gaatattctt tggttgactg gcttagacag ttctctgaag aaaaaagtga
    11101 aagttttata agaggatttt taggtagaat gttattctca ggtgaagaag cattaaaaga
    11161 agctcaagtt atatctggag gagaaaaggt tagatgtatg cttagtaagc taatgctttc
    11221 aaatgcaaat gtacttgtat tagatgaccc tactaaccac ttagacctag aaagtataac
    11281 ttctgtaaat aaaggacttg aaaagttccc aggagtgctt ttatttactt ctcatgacca
    11341 tgaatttata tctactatag caaatagaat tatagagata actccaaatg gaataatgga
    11401 tagaaaaatg gactttgatg agtatttaga aagtaaagag atacaagacc aattagctaa
    11461 aatgtatgga aaagataaat aattattagt ataaaacctt ttaatatttg atacatgcta
    11521 tgcatgagtg tgtaaatttt taatcaaata ttaggaggtt ttttatgttg tttaaatatg
    11581 tttattagca gaatcgagta atattttgta tattaagaat ttaaatataa tatagtagat
    11641 gatataataa aaatatataa cattttgggg ggaatagatt atggttaaga aaaggttagt
    11701 agctgtagta ttgagtttag ctatgattag tgtagggaat ttttcatatg ctcactcagg
    11761 agggcataat tcatcaggag gtcatcataa taaaaataaa tcagagttaa cacgttatta
    11821 ttgtggagga cattcagcac atcaaccaaa taaaggtttt tgtccttatg ctagtacaaa
    11881 agaaaattct ggagtaacaa tatcttcagt acataacgaa aaaaatcata aaaactattt
    11941 tgcagaaaaa gggtataata aaggttatga agatgggtgt aaagggcttt ataatttaag
    12001 tgtcacagaa gccaaatctg ataatttata tgaaaaagag attttaaaaa gttcatatga
    12061 aaaaggatat ttaattggat acaatgaata taaagcaaaa aaagtagatg atgtaatgaa
    12121 gaagatgtta gatattccaa tgttagcaag tatataataa aatagtattt aaaaagatac
    12181 tttctataag gaaatatctt ttttattgta aagtataatt tttcataaaa aagtataata
    12241 actgaggcga taaattaaaa taaaacagat tatttaagta gttattaatt tgagatagat
    12301 acatttaata ttaatgaatc tatctcaata agctaagcta atttaaagct aagataaatt
    12361 aatattaatt agcattaaat aaactttcag aaattttgtc agaagcctct ttatccattt
    12421 ctttaagtac atgagaataa acgcttagag tagtacttat atttgagtgt cctactctct
    12481 cggatataac ctttataggt actttagaat ttataagtaa agtaacatgt gagtgtctta
    12541 aatcatggaa tctaatatga ggtaattcat tcttttctaa aaatctttta aattttttac
    12601 tcataacatc ttcagcaata ggttcacatt tcttattgaa aaacaacaaa ttattattat
    12661 ttctaatgga agattttaat aaatttttat tctgttttat tttatattct tttaataaag
    12721 acattaattc tttgggagaa gatatttttc taacagagct ttctgtcttt ggctctttga
    12781 gaataacaga accattaatt cgggaagtta tcttatttac tgttattgta ttttcttcaa
    12841 aatcaatatt atcccaagtt agacctaaaa cttctgaaat tctaagacct aatccaatag
    12901 ccaaattaat aggtaattcc atttctgtat tttttgctaa atctaataat tttatcattt
    12961 gttctctatc ataaatctca tttttaaatt ttttatatct tggagcctct atactatcca
    13021 taacattttc ttttattaat ttgagtctat aagctctttt tattgcaagt ttcaatatat
    13081 ttatatgaat cttaattgat tgaggattta gtttataaga tagtttatct atgtagtttt
    13141 gtatgtgaac cggatgtaag tcttgtattt tatattttcc aatagatgga ttaatgtaat
    13201 ttttacaaat actaacataa ttattatatg tagctaaaga tatattttca ctatatttat
    13261 ttagaaaatc taatagaaat tctgacactg taatttcgct tggtactaaa aaaccatcat
    13321 tatataagct ttcttttacc tcatttagtc ttttacttgc ttcccttttt ttatcaaaag
    13381 tacccatatt tttttgtttt cttttacctg tcttttcatc ttcaaattcc aggtaaacaa
    13441 catagttttt atttcttttt ctaataaatg cattcatata taacccacca atcaaaattt
    13501 tatacattta ttataacata tatatatcac aaaataattt tgaaatagag ttgcatacat
    13561 tttaatacat tgtatatatt tatttaaaaa atgtatatat tatgtcaaat attagaattt
    13621 taatttgata atctgttata cttatagaat agatatttat ctttatatta atagattgtg
    13681 tgtttaattt tgaataaaaa tataaatttt ttgtattaat aaatttggat gagatatttt
    13741 aaataaaacc tttgactcga aaagtctatt aatatagtat ttaattatgt agaagtttga
    13801 gaggagtgta aaatcatatg actattacta agataccacc agcagaatta aaagtcatga
    13861 aatttatttg gagttcagat tttacagtag catcaaaaga tgtcattgaa gctatggaaa
    13921 aactatataa ttggaagcaa actacaacac taacactttt atcaagatta gtaaataagc
    13981 aattcttaag tgcaagaaag attgatagac atacacatta tacagttctt attggagaag
    14041 aagagtattt acattttgaa acaaaaactt ttttagataa tatgcatgga agttctattg
    14101 agagttttat gaaatccttg ttcaaaaagg gggtaaatga agaagaattg aactctgttg
    14161 aaaaatgggt taaagatatt aaaaaagatg aataatttgg tgtagttaat tatcataaaa
    14221 taaatagttt ttacaataat tagttaaatg aataaaatat aaagttttat gtaaaatttg
    14281 taatttttat aaatattttg tcttgttatt taatattaat acaataagtt tgatgtaaaa
    14341 tcaaacctgt ttgaatttaa tttatattgg gtttgtcatt agtattattt tagtttgata
    14401 ttgtatacga atttaaaagt cagaattttt gtatattaat catgtaaatt aattgttaga
    14461 tataaagggc
//
LOCUS       DAEPOZ010000094        14415 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.094, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000094 DAEPOZ010000000
VERSION     DAEPOZ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14415
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.094"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(177..932)
                     /locus_tag="KR440_002028"
     CDS             complement(177..932)
                     /locus_tag="KR440_002028"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBH0699077.1"
                     /translation="MLKVIINGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVIFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            1373..2362
                     /locus_tag="KR440_002029"
     CDS             1373..2362
                     /locus_tag="KR440_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBH0699078.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMIVLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVINATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
     gene            complement(2633..3349)
                     /gene="dapD"
                     /locus_tag="KR440_002030"
     CDS             complement(2633..3349)
                     /gene="dapD"
                     /locus_tag="KR440_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBH0699079.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     SNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLNEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(3573..4322)
                     /locus_tag="KR440_002031"
     CDS             complement(3573..4322)
                     /locus_tag="KR440_002031"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBH0699080.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDNEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(4389..5276)
                     /locus_tag="KR440_002032"
     CDS             complement(4389..5276)
                     /locus_tag="KR440_002032"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBH0699081.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(5393..6394)
                     /locus_tag="KR440_002033"
     CDS             complement(5393..6394)
                     /locus_tag="KR440_002033"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBH0699082.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            complement(6819..7700)
                     /locus_tag="KR440_002034"
     CDS             complement(6819..7700)
                     /locus_tag="KR440_002034"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBH0699083.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
     gene            8023..9216
                     /locus_tag="KR440_002035"
     CDS             8023..9216
                     /locus_tag="KR440_002035"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBH0699084.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            9422..10540
                     /locus_tag="KR440_002036"
     CDS             9422..10540
                     /locus_tag="KR440_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HBH0699085.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            10671..10916
                     /locus_tag="KR440_002037"
     CDS             10671..10916
                     /locus_tag="KR440_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HBH0699086.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            10903..11649
                     /locus_tag="KR440_002038"
     CDS             10903..11649
                     /locus_tag="KR440_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBH0699087.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            11934..12809
                     /gene="hslO"
                     /locus_tag="KR440_002039"
     CDS             11934..12809
                     /gene="hslO"
                     /locus_tag="KR440_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBH0699088.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPDDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            13200..14000
                     /locus_tag="KR440_002040"
     CDS             13200..14000
                     /locus_tag="KR440_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBH0699089.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
BASE COUNT         5295 a         1914 c         2291 g         4915 t
ORIGIN      
        1 taataaacaa tataattcca tataccaata aataagataa tctttaagaa aaatgattat
       61 atttaaaagg taagcataaa atttataaac ttttagaata gaaatctcaa tcaattagat
      121 attcaaattg atgaatatga atacctaaat agattgagat ttattatgta caaaacttat
      181 cctagtatat ctgacatttt ataaagtcca gattgcttac caattaaaaa tttagctgca
      241 actatagaac cgtttgcaaa tacttcatca gattgagaat gatggcttaa tgtaagaact
      301 tcattatcac ctgcaaatat gacatcatgg tcgctgatta tattgccacc tcttatagag
      361 tgtattccaa tttcatttgg ttttctttta caatctctac catatctacc atgtgtattt
      421 tcactttcag gcaagacttc tctaattgca ttagctatca tagtagcagt tccactaggg
      481 gcgtcttctt ttctattgtg atgtttttct atgatttcaa tatcaaaacc ttctaacatc
      541 tttgaagcat ccttaactaa ttttattaat atattcactc caagagatgt atttgatgat
      601 aataatatag gaatgttttt tgatgcttct tctattttat ttaagtcatt ttcatcatat
      661 ccagtagtag ctaatactac aggtgtatta ttttttagag catatgacaa tattggctct
      721 aaagtctcat gatgtgaaaa gtctattatg acatcagttt tttcagttac atcagtcatt
      781 gttttgtaaa gctttgtatc actatgatga cctttttcat cagcagacct agctacacca
      841 caaaccaact ctaattcatt atcttttttt acacattctg ctaaaactct tcccattgtt
      901 ccattatagc cattaattat aactttcaat ataaattcct cctatgaaat taataaaaat
      961 ttgttatatt tctataagtt tattttatca ctaatattat ttatataaaa taatattagt
     1021 gataataagc ttacaattta tcttacatta aaaaaattta agtggatttt tcaaaatata
     1081 gataaacttc tttttcattg tataaatgat aaaaatatat ttgacttcat atcaaaaaga
     1141 gtgccccatg aactaaaaag ttaattttga gataccatat tcatatatca tatgatttat
     1201 ttggaaatca atattaatac attaaaataa ctaaatacat gtatgcataa agatgtataa
     1261 aaaatatata aacatatagt attaatttta aattcaacaa tttaattgtt aaaagtgtta
     1321 ttattaaatt agaaatatct taacttaata tactgagatg ggagtgatag atatgttaaa
     1381 agaagaaaaa acttatcaag agaaagacgc aaaagtattc aatggagtgg cagctttgat
     1441 aatgattgta ttatttttag tagtttcaat aggtatcatg gtttttggga tagtaaaact
     1501 tgatagtgga tttactggaa taggttcact tatgttaatt ttgggacttg tatttatagt
     1561 agtagatttt atcctatttt ttgggctaaa aatgattaat cctaaagaag ctatagttct
     1621 agttttgttt ggtaactatt atggaactat aaaaaaagaa gggtactatt gggtgaatcc
     1681 attttgttca gcaataaacc cagcagtatc aagaattaat gtatcgaaat cttcatctga
     1741 tgaaaaggta gatatatcaa ctaattcaag gggcaaaaaa gtatctttaa aaacaatgac
     1801 tcttaataat gaaaagcaga aagtcaatga tttattggga aatccaatta taataggagt
     1861 ggttgtaatt tggaaggtta taaatgcaac aaaggcagta tttaatgtgg ataattataa
     1921 tacattctta tctattcagt gtgattctac tattcgtaat gtttcaagac tttatccata
     1981 tgatgtatct gaagatggag atgaaaaatc tcttcgtggt agtagtcagg aaatagcaga
     2041 tagattaaaa gatgaattac aatcaagggt tgacattgca ggaattgaag tgtgtgaagt
     2101 gagaattact catctttctt atgcaccaga gattgcagca gcaatgttac aacgtcaaca
     2161 agcagaggct ataatagcag cacgtaaaaa gattgttgag ggggctgtaa gtatggtaga
     2221 gatggcattg actaatttaa gtgaaaataa tgttgtgact ttggacgaag aacgtaaagc
     2281 agctatggta agtaatctgt tagttgtgct ttgtggcaat aaagatgcac aaccagtagt
     2341 aaacagcagt tctatttatt aaattataaa aagtttaata gtttattaca ttgatatatt
     2401 gaaaaattga aggaaataaa aatacttgtc atcaagaaat aaaaatgata taagaatgaa
     2461 taagagaatt gtaaaaaaat aaaatgatta taaataaaag ataaaaatat tataaaaaag
     2521 agggtgcctc tgatgaatta aattcataca gagacacctt tttaattaat tttttaaatt
     2581 agtatattat tgtttaatta atctttctaa ttaataatat agtactattt aattagtcta
     2641 aatttctaag gtcttccata agcttagttt tgtcagccgt tttttcatct ttcattttta
     2701 taactttagc aggagaacca gcaacaacgg caccagcttc tacatcagta gttacaacag
     2761 caccagctgc aacaacagca cctttaccga ttctaacacc ttcaagaata actgcattag
     2821 ctcctattaa aacatcatct tcaactataa caggagtagc agaaggtggc tctaaaacac
     2881 cagcaacaac agcaccagca cctaagtgaa catttttacc aagagttcct ctagctccaa
     2941 taacagcatt catatctacc atagaacctt caccaataac agctcctata tttataacag
     3001 ctcccatcat tacaacagca tttttttcta tagtaaccat gtctcttatt attgcacctg
     3061 gctcaattct tgcatgttca tttaaagtat tttttagagg aatagcagaa tttcttctat
     3121 catattctac atgagtatct gttatatcat tcccatgttt atctaatact tccattatat
     3181 cctcatagtc acctacaaat atataagaac cattacttcc aaatacctta aatttatcag
     3241 ttgattggaa gtgtaaagag ttagtattta catatacctt aacaggtgtt ttctttttaa
     3301 cttcttttat ataccttgct atttcataag catcgtttaa atttaccatt tgttaattct
     3361 ccttgtattt cattatattt cttttattat atacttattt gtcaatttta gtatatactt
     3421 acttcttatt atttatattt tattttagtt agtttttatt gtatagacat agtattaaat
     3481 aaatttatta tatatgtttg atttgtattg cgtattatta tatctatatt gcatatgttt
     3541 aatttatatt gtgtattatt atatttatac tattatccca acatgctgtc catattataa
     3601 aatcctggtt tttgcttaac taagtatttg gcagcagcta tagaaccact tgcaaagata
     3661 ccacgagatt gagcttgatg gcttattgta agaacctcat tgtctccagc aaatagagca
     3721 tcatgttcgc caacaatatt tccacctctt atagcatgaa taccaacttc atttggttgt
     3781 ctttttgcac tacgaccata tctaccataa ttattttcaa cgtttggcaa aacctctttt
     3841 atagcgttag caatcataac agctgtgcca cttggggcat ctacttttct gttgtgatgt
     3901 ttttctatta tttctatatc aaatccttct agtaatttag ctgcatcttt aaccaatttt
     3961 actaatacat ttacaccaag agacatatta tatgattgga atacaggaat aactttagcc
     4021 gcttcacgta ttttgttcat ttcgttatca ttatatccag tagtagcaat tactaaaggt
     4081 gttttagtct ttaaaacgta agataagata ttatctaaat tagagtgatg agaaaaatct
     4141 ataactacat cagcttcctc aactatagta ctcatatctt cataaatttt aaggtcgcct
     4201 tcatagctat ccagacctct agctgcaaca caaaccaatt ctaattcatt atcttctctt
     4261 atagtatcgg ctaaaatctt acccattttt ccatcataac cagttgctat aacttttaac
     4321 ataatttcct cctatgatta agggctaact tttgaccaaa cttacttttt accatgcatt
     4381 aatctttact atgcattaac ttttagtcca aagttaacta attctttctt taacacttct
     4441 aaatttgctg ggtccatttc tgctagaggt aatcttaaat ctccaacatt gaatcccaat
     4501 aaattcattg cagtcttaac tggtatagga tttacttcta taaataaagc tgcgattaga
     4561 gcatccatac caagttgtaa ttttctagaa ccttcaatat cgccttcaaa gaatttagct
     4621 accaaatcgt gagtttcttg agggcaaaca ttagcaagta ctgatataac tccttgacct
     4681 cctaaagata ataaaggaag tatagtatca tcattaccag agtagatagc aaagtcatct
     4741 ggaactaaac tagctatttc tgcaacttgt cctaggtctc cactagcttc ttttacagca
     4801 actatgttat caattttagc aagttcaact ataaggctag gacttatatt tacacaagtt
     4861 cttccaggga cattgtaaag tataattgga agtttaacac tgttagctat tgtttcaaaa
     4921 tgacgcttaa gtccagcttt attagcttta ttgtaataag gtgttattat taatagagca
     4981 tctactccta atttttcagc ttcttgactt aagttaactg agtgcatagt atcattagaa
     5041 ccacttccag ctataacagg aactctttta ttaacttttt caacaacata cttaatagta
     5101 gcaagttgtt cttcatcagt catagtatta gcttcaccag tagtaccaca agatactata
     5161 gcatcagtac cattagctat ttggtattca actaattcac ctaatttgtc aaaatcaact
     5221 ttgttatcct ttgtaaatgg tgtaactaga gcaacagcag aacctttaaa tatcatataa
     5281 aatcaatccc ctctttttta tttttgtgtt ttatattaca gttacttcta atgcttgttt
     5341 ttgagttact attaatattt tacaaaatta tatatttata caagtttgta tattatacta
     5401 aatcgtatga taaagccatt tcagctattt gaattgcgtt agttgcagca ccttttctta
     5461 tattgtcagc aacaacccaa agatttacac cattatcaac actaaaatct cttcttattc
     5521 ttccaacaaa tacttcatct ctatcatcaa attctacagc agttggatat tcgtttttag
     5581 caggattatc aactactaca acacctggag cttcttcaag tgtttttact aaatcatcta
     5641 actcaaatgg attgttaaac tcaacattta tagattcact atgaccattt tttactggaa
     5701 ctcttacagt agttgcagtt atttttaaat catagttatc aagtattttc atagtttcat
     5761 ttaccatttt catttcttct ttagtgtatc cattttcagt gaatgaatct atatgaggaa
     5821 gacagttgta agagatttga tgagggtaga atccagtagg attaccgttc attcctttct
     5881 ctaaatcttc aacaccttta acaccagaac cagatacagc ttggtaagta gagtaaacaa
     5941 ctctcttaat tttgtattta tcatcaagag ctttaagggg aaccatagct tgtatagtag
     6001 agcagttagg attagctatt atacccttat gattttttat ggcttctgga ttaacctcag
     6061 gaactactaa aggcacatct ggattcattc tccattgact agagttatct actactaaga
     6121 ttccatttga agcagctatt ggagcatact tttcactaat acttccacca gcagagaata
     6181 atgctatttg tatgcctcta tcagtaaatg aagtttcctt taattcttct actatatatt
     6241 ctttaccaca aaacattact ttagaaccag ctgatttaga agaagagaat aaaaatagat
     6301 tttctattgg aaaatttctt tcttctaata cttttaaaaa tgttctacct accattccag
     6361 tagcaccaac tattgctaca tttacttttt tcatataaat tcaccccaat tttaaattat
     6421 taaaacatta ttttatttta gttatacaag taagatatgt taatattttt atatatccaa
     6481 aaactttaat attatattaa caactttaat tatatacaaa aaacgtacaa aggtatactt
     6541 tgtacgtcta atttatcact caaagtaaac cctgtagctc atcacctaca aaaataagtg
     6601 acagtattac acatgttatg tgcaatccca gaaaatatta ttgcaagaaa tattttcttc
     6661 ggctgtaaat cctttttcca atcattaaca cctgccagct actattggat actcttagtt
     6721 acagctacct ctaccctagg ttaataggat attaaaattt gattattcta ataataatac
     6781 atatttaaga aaaaatcaac ttatttatat aaatattgct aaaacttaaa tccaaaattt
     6841 gtcaattctt gttttaacac attaagatta gtttcttcca tttcagctag aggaagtctt
     6901 aaatcaccaa cattaaaacc taaaatgttc atggcagttt taacaggaac tggatttact
     6961 tctataaata aagcagcaat taaagcatcc atatcaagtt gtaatttttt tgagccttca
     7021 atatcacctt caaagaattt agttactaaa tcatgtgttt ctttaggaca tatgttagca
     7081 agtacagaga taacaccact tccacctaat gataatagtg gtagtatagt atcatcatta
     7141 ccggagtata tagcaaagtc tttaggtact aatttagcta tttctgcaac ttgagctaag
     7201 tctccacttg cttccttcac agcaactata ttatctattt tagcaagttc ggccacaaca
     7261 gatggcttta tattcacttt agttcttcct ggtacgttgt ataaaataat tggcaactta
     7321 acgctatttg ctattgtttc aaagtgaagt ttaagacctt tctcattagt cttattatag
     7381 taaggagtta ttattaaaag accatccacc cctaattttt cagcttcttg actcatatga
     7441 acagacaaca tagtattgtt tgaaccagta cctgctataa caggtattct cttattaact
     7501 ttttcaactg tatattttat tacagcaaaa atttcagatt cactcatagt agtcgcctca
     7561 cctgttgttc cacaaacaac tagtgcatct gtgccattct caatgtgata ttcgattaat
     7621 tcacctaatt tttcaaagtt gacattatta tcctctgtaa atggggtaac taaagcaaca
     7681 gcagaacctt taaataacat ataatcaaat ctcctttttt atctaaattt aattttttaa
     7741 gtacaataat aattatagta caaattaatt aattttaata ggtatagtta atatcttaac
     7801 tgtttaaata agatatttat attatattaa tattagaaaa attttaaata aatgttttat
     7861 tctcaaaata tgcatattaa tttaataaaa tataccaaaa tggaaataaa aaataaattt
     7921 tatgagaatt taataactaa taattaatat atttaggtta taataaatat gtgcgtaaaa
     7981 tctaacaata agcaataata ccgtaaattt aggaggaata atatggatac tttaaaggaa
     8041 ctttttaaaa taggaagtgg accttcaagt tctcatacta tgggaccaca aagagcagca
     8101 gagagattta agaatgaaaa cccagatgca gaaagtttta gagcaatatt atatggtagt
     8161 ttggcagcta cagggaaagg tcacttaact gactatataa ttgaaaaaac tatagcacct
     8221 aaaaaagtcg aaattgtttg ggaagaagat attataaaag attttcaccc aaatgggatg
     8281 aagtttgaag ctttagataa agataaaaat gtaacagctg aatggactgt atattctgta
     8341 ggtggaggga ctatagcaga agaaggtcaa agaaacagta aaagcaatag tatatatcac
     8401 ttagatacta tggatgagat agtaaaatgg tgcaaagaaa ataataagac tttggtagat
     8461 tttgtgttgg aatgtgaacc aaaagacata aaagattata taaaaactat aaaagatgct
     8521 atgagaaaat ctattgatga tggactaagt acagatgaaa taataccagg caagttatta
     8581 ttaaaaagaa gagcaagtac attttataat gcatataaaa aagataagag cttctcaaca
     8641 cttgtatatg catatgcact agcagcttca gagcaaaacg cttctggtaa tataatagtt
     8701 acagcaccaa cttgtggttc tgctggagta ataccaggaa tattctttgc aatgcaggat
     8761 ttctataatt atgatgacga aaaaataatt gaagcactac ttgtagctgg aataataggt
     8821 aatataataa aaactaatgc ttcaatatct ggagcagagg tagggtgtca aggtgaagtt
     8881 ggagcagctt gttctatggc agcagcagca gtagcatact taaaaggtgg aactatagac
     8941 catattgaat atgcggcaga aatagcactt gaacatcatt taggaatgac ttgtgaccct
     9001 gtatatggat atgttcaaat tccatgtata gaaagaaatg caatggcagc tcaaagagct
     9061 tatgatgcag ctaattatgc attattaact gatggctcac attctgtctc cttagaccaa
     9121 gttgttgaaa caatgaaaga aactggtata gatatgatgg ataagtacaa agaaacagct
     9181 aagggtggac ttgctaaaca tttcttctct tgctaaaaat aaaattttcc aataataatt
     9241 cctctgtttt gatttatgaa acaggggaat tattattaat ggtgaattat ttaataatgc
     9301 aaaatatttt tatattcaat ttagcttttt atttttaatt agtataacat gctatataat
     9361 attttaatta taaagaaata tagaaatatt ttttaataaa ttagaaattg gaggagatta
     9421 catgagagag gcatcatatt tacaagaaca actttataaa cataggaagt cattaaatcc
     9481 tattgcagag attgggagaa aagagtataa gacttcaaaa tatataagag attatcttga
     9541 taaattagga attgagtatg aagtttattt agaaacaggt atagtaggtg taatagaagg
     9601 taaaagacct aaaaaagata ttgcttttag agctgacata gatggtcttc atagtgaaga
     9661 aggggtaaaa catttatgtg gtcatgatgg acatgctact atattacttg gattaattga
     9721 gtatttaaat gataataaag aagacttaaa tgataatata gtatttatat tccaaccagc
     9781 agaagaaggt cctgggggag caaaagcttt aatagatgaa ggagtgctta gaaaatacaa
     9841 tattgatgaa atatatgggc ttcatatata tccagagtta ccagaaggtt atgtaggtac
     9901 aagagaaggc tattttatgg ctcaaatagg agatatcgac atagaaatag tgtctaaaag
     9961 tggacatggg gctatgccac aaaatggtat tgatggtata gtgatagctt caaactttgt
    10021 aagctcactt caaactatag tgtctagaaa tataagccca atagacaatg cagtgttgac
    10081 tattggtaga attgaagggg gagctagaag aaatataata gctgaaaata taaagatgga
    10141 aggtactatg agatgtttca gcccagaagt gtatgagaaa atgaagctta gagttagaga
    10201 acttgctaga gggtttgaat tggcttataa ctgtaaggtt aatataaata ttatagatga
    10261 ttatattgct gttaaaaatg acaaggattt gtatcaagag tttgtagagg ctattggaag
    10321 tgatactgtt gtagagttag agccacttat gatatcagaa gatttttctt attatcaaaa
    10381 agaagttcca ggattatttt ttatgttggg ttcaagaaac gaagaaaaag gatttgtaaa
    10441 tggtcttcat aatataaact ttaattttga tgaaaagata tgtgtaaatg cattgaatgt
    10501 ttattcaaaa ttattaaaat acaaagaagc aatagattag gaagataaat tataaagatt
    10561 aaaaagcata aatacctaaa tttaaaaaaa atgtattaat aaatacccct tatatatttg
    10621 tataaattta ttttaattga caaaaataca aataagaagg ggtgattttt ttgaaagaaa
    10681 aagtacaaag agaaaagaag ataactaatg gcaagaatac taaaatattg acaaatgatg
    10741 atataatgaa atatgagata gcctcagaac taggtcttat ggataaagtt ggtgaacgtg
    10801 gatgggcagg acttacagct aaggaagcag gaagaattgg aggtatgctt acatcacgca
    10861 aaaagaggct taaaaagatt gtggaggagc agaataaaaa ggatgagtca atatagtgat
    10921 tttgcgtttg tatatgatga acttatgaat gaagttgatt ataatggttg ggttaaatat
    10981 atagaagata taatagatag tgaaggtgta aaagtaaaaa atatattgga attggcatgt
    11041 ggcacgggta atcttacaat tccattgact aaaaaaaatt acgatatagc tggtatagat
    11101 atatctgatg agatgcttag tgtagcaaga gaaaaggcag aaaaagaagg agtagaatta
    11161 gttttacttc aacaagatat atcagagctt gattttgaaa tatctgattt ggactgtgtg
    11221 ctttgtgctt gtgatggatt taattatata acttatgatg atgatttgga aaatgtattt
    11281 tcaaagacat atgaattatt aaagaaagat ggaatattca tatttgatat aagttctttt
    11341 tataaattag ccaacatatt gggaaataac atgtatggag aaaatagaga agatatagcg
    11401 tatatgtggc aaaattactt tgatgaagaa gagaatctaa ttgaaatgga acttacattc
    11461 tttattaaag atgaatgtgg aaagtttgaa agatttgaag agatacacca acaaagagca
    11521 tatactgaag atgagatagt tgatatgctt gaaaaatctg gatttaagga tataaaatat
    11581 tatggggatt ttacatttga caaacccagt gaagaaagtc aaagaatctt ctttgtatgt
    11641 aaaaaataag atatttttga ctatataaga taattattaa aaataaccat gatataaaaa
    11701 tatagaaaat gcagttttat agattaaaag taaagtgatg atactttatg acttttattt
    11761 tctaataatt ataaagttct atagataaaa attaaaatat aataactatt gcttagtaaa
    11821 attaaaataa aatgggtttt gcccaaggtt aatatagata agtacttata gattaaaatt
    11881 aaggtagtaa tataataaaa ataagacaat taacgggaca tggaggaata ttaatgagag
    11941 attatgtact aagagcgaca tcaggaaatg gtcaagttag agcatttgtc gcaactacta
    12001 gaaatacagt agaagaagct agaagattac atgaaactac aaaagtagca acagcagcac
    12061 ttggaagaac attaacagcc acatctataa tgggattaat gatgaaaaat gatagtgata
    12121 aattgactgt aataataaaa ggtggaggtc caattggaac tattatcgcc acatcagatt
    12181 ctaagggaat ggtaaaagga tatgtaggaa atccacaagt agaggttgaa gactatccaa
    12241 atggaaagtt aaatgtagca gcagcagtag gtacagaagg tgttgtaaaa gttataaaag
    12301 acttaggtct tagagaacca tataatggga cttatccatt ggtaagtgga gaaattgcag
    12361 aagactttac ttattatttt gctgtatcag agcagactcc atcagtagta gctttaggtg
    12421 ttcttacaaa agaagatgaa gtagagtttg ctggaggatt tatagttcaa ttaatgccag
    12481 atgcagaaga agagactatt gctaagttag aggaaaatgt agctaaatta ccttctataa
    12541 ctaacatgtt aaaagaagga aaatcaccag atgatatatt aaatattgtt ttggatggtt
    12601 tggaacctaa aatacttgat acctgtgaag tagggtttat gtgtgaatgt tcaaaagaaa
    12661 gagtaaaaac tgctttggtt gcaattggta aaaaatcatt ggctcagata atagaagaag
    12721 ataaaaaagc agaagttggt tgccagttct gtaataagaa gtatatgtat agtgaagaag
    12781 agttgcttga aattttaaag gaaatgtagg ctataaaaat agttgtattg atataagtaa
    12841 tgttatagtg atatagtaaa gtcgtattga tatagtaatt aaagtataat ttattaataa
    12901 tagacatatt tatattaaaa aataaattta ataaattttt aagcttaata aattttaaga
    12961 attaagaagc tttctggctg taatatttgc aatgttgcag ttagaaggct tttttatttt
    13021 attatagttc gtgtcaaaga ttgtatgaaa caataaaata ttgtaaaata atttgtatat
    13081 ttagacctga ttgacacaaa ggtttataag atatttgagt tgattatacg aaattctatg
    13141 aagtagtaac cctgtgactt taatcatggg aggttcagaa gaaggagaaa attttaaata
    13201 tggaaagtaa aacgaagtac ataggaataa gtatattaat attattgatg atatttttaa
    13261 ttgcaaagaa ttttaacgga aaacttgaaa tacctattcc tgagagtaat agtagaaaat
    13321 ttaaatcagc agcagtgttt tatcctcaac atcaagatga tgaggtttta tggggaggaa
    13381 gtgccattgt agatgctata aaacagtgtg gtgttgataa tgtatatgtg gtattagtat
    13441 cagatggttc tggagttaac gttttcaaag ctaacacaaa atttagaaac ttaactagga
    13501 agcaaaagga agagcttaga aataatgagt ttaaatcagc tttgagagag cttggagtga
    13561 aacctcaaaa tgtgattata cttgcagata tagataaaaa ggaaggcaca cattatgaat
    13621 taatggaaaa gactatattg gattttgaac ataagtttaa aggcaatgtg acccatatag
    13681 cccatcacta taagtatgat gaccatataa tgcatagaaa gaatggggaa gttttaaaga
    13741 aacttaaacg tgaaggtaag ataaaagatg acttgtattt tgttaagcca aaatatgtaa
    13801 agtatatacc aagaaatgaa agagctatct ataaagcaat ggattttgat gactacaata
    13861 aggttaagag tgcttgttat gaatataagg ttgtaaattc aaagcgtaaa atgtatggta
    13921 taggatatat ttcggcacat agttattttg aacatcttct aaatgaccct gaacttacgt
    13981 ctgtattaag tgaagattaa tttcttataa gtagtaagtt atgtttgata agttgattgg
    14041 aattttctat agttttgaag gtattggaaa tttagtttta tttaaagatt taaagataga
    14101 ggtttttaat gtttaaaatt ttaaagttaa gttgaataat tgtatagaat tatttgtgaa
    14161 agaagaaaca gtgtaaaaca tagtatctta tttatggttt taaaatcata ggcaagatac
    14221 tttttttatg taactaatac cattttgagg ttattaactt aagaatttta tataatttaa
    14281 cactcatata taatgatgat atttaggtag aatagatgca attacgctta aataaaataa
    14341 ctgtattttt tataaaaatc aaaccctaca ctatactacc agataaaatc tacatgctat
    14401 aatttgacct gtgtt
//
LOCUS       DAEPOZ010000095        14409 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.095, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000095 DAEPOZ010000000
VERSION     DAEPOZ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14409
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.095"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            303..2180
                     /locus_tag="KR440_002041"
     CDS             303..2180
                     /locus_tag="KR440_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0699090.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKTVYDEVIKIVPQSNDVGKKNESQEKQSIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSFIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            2492..3898
                     /locus_tag="KR440_002042"
     CDS             2492..3898
                     /locus_tag="KR440_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBH0699091.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNTVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            3926..4774
                     /locus_tag="KR440_002043"
     CDS             3926..4774
                     /locus_tag="KR440_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0699092.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            5435..6127
                     /locus_tag="KR440_002044"
     CDS             5435..6127
                     /locus_tag="KR440_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0699093.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            6124..7059
                     /locus_tag="KR440_002045"
     CDS             6124..7059
                     /locus_tag="KR440_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0699094.1"
                     /translation="MKMDEQTIILFLFLLSIGLLFLSIFAFSKLGHIHKRLKDIEEIL
                     ADVQIGNENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIGQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVDDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQISVESEIGKYTVFTVSLPLI"
     gene            7207..8130
                     /locus_tag="KR440_002046"
     CDS             7207..8130
                     /locus_tag="KR440_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0699095.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRKFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAHAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            8114..8884
                     /locus_tag="KR440_002047"
     CDS             8114..8884
                     /locus_tag="KR440_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0699096.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVPKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVTVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPLLSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            8894..9676
                     /locus_tag="KR440_002048"
     CDS             8894..9676
                     /locus_tag="KR440_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0699097.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGIAVLLSLFSFLCTFILGLLFCNINISVTLIVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            10053..10589
                     /locus_tag="KR440_002049"
     CDS             10053..10589
                     /locus_tag="KR440_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBH0699098.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            10621..11010
                     /locus_tag="KR440_002050"
     CDS             10621..11010
                     /locus_tag="KR440_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBH0699099.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            11047..11433
                     /locus_tag="KR440_002051"
     CDS             11047..11433
                     /locus_tag="KR440_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HBH0699100.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGSQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            11560..13002
                     /locus_tag="KR440_002052"
     CDS             11560..13002
                     /locus_tag="KR440_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBH0699101.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVENESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLIDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            13299..14114
                     /locus_tag="KR440_002053"
     CDS             13299..14114
                     /locus_tag="KR440_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0699102.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINNFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKRVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT         5215 a         1755 c         2705 g         4734 t
ORIGIN      
        1 taatatacca tttagatttt ttaatcaagt actttctcta ttaaaaaaaa tttaaatata
       61 taattattat ttacaaaaaa attatatttt atagttgaat taagaaaacg cttatgatac
      121 tataaagtta acaaatttga atatataaca gtagacgtgt tactggtaat gcaggcaaga
      181 tcgaatcaat ttttatagat tatctataga gtactatggg tataatctat ttatttggtt
      241 cgattttttt gtttaattgg ccataaaaca aaaagcctat agttgaaaat ggaggatttt
      301 ttatggatta taaaaatata gctcaagaaa ttctactaaa tgtaggagga aaagagaatg
      361 taaatgaagt gactcattgt atgacaagac ttagatttaa agtcaaaagc gcttctaaag
      421 tgaataagga taaattatct aaaactgaag gagtaataac tgttgttgag agtatgggtc
      481 aaatacaagt tgtaattgga aataaagtaa aaacagtata tgatgaggtt ataaaaattg
      541 tacctcaatc aaatgacgta ggtaaaaaga atgaaagtca agaaaagcaa agtataatta
      601 attctatatt atcagcagta gctggtatat ttacacctac aatacctgct atagcagggg
      661 ttggtatgat taaaggtatt ttatcagttc ttgctatgta ttatatgaat aaaaatggag
      721 tagatataaa agaaacgcaa agttatatta tcttaaatgc catggccgat tctatattct
      781 attttatgcc tattatacta ggatatactg cggctaaagt atttaatgca aataaaataa
      841 tatctatggt attaggagct acactttgtt atccaacttt tactgctttg atggcaggtg
      901 aagaatctgt tagattttta ggattggcag taacaaaagc tacgtataca tcatctgtta
      961 ttccaataat tattgctata tgggctcttt catacgttga aaaagtatta gaaaagtata
     1021 tacctgaggt aattaaaata ataatggttc caactttatc attggttgta atgttacctg
     1081 caacattatt tttatttgga ccaatcggaa tttatatagg aaatgtcata aacttttctt
     1141 ataagtatat ttatgaatta agcccagcgt tgtgtggagc atttgtaggt gggttatggt
     1201 gtgtgttggt tatatttggt gctcatagag cattattacc aataggtata agtgatgttg
     1261 ctcaaacagg aagacagaat ttattggcat ttgcaggagc tgcaaacttt tcacaagcag
     1321 gggctgctct aggagtattc tttaaaacta aaaatcaagg actaaaaaca atttctatgt
     1381 ctgcaactat aacagcttta tttggtataa ctgaacctgc aatatacggt gccaatctta
     1441 gattaaaaaa acctatggta tgtgccgtaa tatgtggtgc atttggaggt gcgataatgg
     1501 gaatgggtgg agcttatggt aatgcatttg caaaccaagg tgttttaact atacctgtat
     1561 atgctgaagc aggtgcatta ggtttcttaa gttatctagg tggttgtgct atagcatttt
     1621 ttggttcagc tatttcaact tatctagttg gatttgaaga tttggaagag tcttctaata
     1681 aggaaaattc atttataaaa gtagaaacga aggatggtgt tattgatata acttcacctg
     1741 tagagggcga atgtatagaa ttgtcagaag taaaggatga tgtatttgct tcaaaagcta
     1801 tgggagaagg tatagctgtg ttacctacaa aaggagtaat aacagcccct actgattgtg
     1861 aagtagcttc tttatttcca acattacatg caataggatt aaaattagat aatggagcag
     1921 aaatgttaat acatgttgga atcaatacag tagagttaaa tggtaaatac tttacaaaac
     1981 atgttaatca aggagattta gtgaaaaaag gtgataaact tatatcgttt gatatagata
     2041 aaataaaaaa ggctggttat gatgtaacta cgccagttat agtaaacaat acatttgatt
     2101 ttgggcaagt agtttcttgt aaatctagtt atgtttcaac caatgataat ataatttcat
     2161 tagtattagc tggtaattaa gttaatttga ctagagataa gtaaattgat ttaaaaggaa
     2221 ataagtaaat caacaattaa atacatctaa aagtaagttt attgcaatga attgttaaag
     2281 aaaataatta atatggagta aagctattgc aatgaagtaa caaagaaaat aattaatata
     2341 gagtaagttt attgtaatga attgttaaag aaaataatta atatagggta aatctatctc
     2401 aatgaaataa caaagaaaag agttaatata gagtgaactt attgcaatga atcaacaaaa
     2461 aaaataatca atataaaaag gagaaatatt tatgaataca ggatttccaa aagacttttt
     2521 atggggagca tcatcttctg catttcaagt ggaaggagct tgggataaag acaataaagg
     2581 aaaaaccgta gccgactata attcttttaa aaagtctcat ttacaggcag atactaaagt
     2641 tgcatcagac ttttaccata attatgaaga agatattgaa ttaatgaaag aattagggat
     2701 gaaaacatat cgtttttcaa tttcatgggc aaggataata ccagatggtg aaggagagat
     2761 aaaccagaaa ggtttagact tttataataa gataatagac aaactaatag agtgtgatat
     2821 tgagccattc gtaactttat accattttga ccttccattt aaattagtag agaaatataa
     2881 tggttgggaa agtagagaaa ctgtctatgc ttttgaaaga tttgctaaaa tatgctttaa
     2941 gcattttgga gatagggtaa agtattggca acctcataat gaacagaatt taattgttag
     3001 agtggaggag agaattaata tatatgatga aacagattca tggaagatag ataaaatgcg
     3061 tgctcagatg gattataatc tttgcttagc acatgcatta gcagtcaatg catgtcatga
     3121 gatgataaaa gaaagtaaaa ttggagcagc agtatcatca tctgtaacat atcctcttac
     3181 ttctaagcca gaagatgtat atgcagctag aatgaatgat aattttaaag tgtactatat
     3241 gcttgatatg catcattatg gagaatatcc agggtattat atgaagtatc ttgaaaaaag
     3301 aaatacagtt cctcacatgg aagatgggga taaagagata cttaaaaaag ctaaaatgga
     3361 ttttatagct gtaaattatt ataggacaaa ttgtgcagaa gctttacctg aagattcaca
     3421 acatccattt ggattaagag aaggaactgt tgattttagt atgtatggac tatttaaaat
     3481 gtcaatgaat cctaacttag aggcttctga gtatggagca gcaatagacc catcaggtct
     3541 tagagttgcc ttaaatgaat attggcaaag atatcactta cctgttataa taacagaaaa
     3601 tggtctagga gctaaagata tattagaaga tggaaaaatt catgatgact atcgtataga
     3661 ctatttaaga agtcatataa atgcatgtaa actagctatt gaagatggtg tagagatgat
     3721 tggatattgt ccatggtcat ttacagacct tttaagttct tctcaaggtt ttaataagcg
     3781 ttatggactt gtttatataa atagaactga ccatgaagtt ttagatttaa aaagaatcaa
     3841 gaaagatagt ttttactggt ataaagaagt tatagagaac aatggaatag taaaataaac
     3901 ataaatatat attgagggtt ttagtatgta cattacaaag gtattaaata attcgttgct
     3961 acttgcaaag gatgataatg atgaagaaat aatattgatg gggaaaggca ttggccataa
     4021 ttataaggcc gggtctgagt taaataaaaa agatatagaa aagatttttg ttcttcatga
     4081 tgaaaatata aaaaagagta ttatacaatt ggcaacagaa atagatgcag aatattttgg
     4141 tatagctcaa atgataattg catatggaat agaaaaatat aacttaaaac ttatgaacca
     4201 tatatatttg gccttaactg accacattgc ctttgcagtg agacgcttta aagaaggtat
     4261 aatgattgaa aatcactatt tgttcgaaat caaggaattt aatccaaaag agtatgatat
     4321 aggaaaatat gctattactg tttttaagga ggttcttggt ttagacctac cagaagaaga
     4381 gattggtaat atagcaggcc attttataaa tgcacaacaa gataatcctt atagtgatag
     4441 aaataagaga agtgctaaaa ttgtaaatgc tattttgcaa atagttcatt atcacttttc
     4501 tattgtatat aataaagagt cattttatta taggagattt gtaatgcatc taaaggcctt
     4561 ttcacaaaga tttctggcta atgaaccatc aagagaaaaa atagatttta tatatgagca
     4621 agttcaaaaa aattgtaagg aagagtatga atgtgtgaaa aaggtaggag cttatatgta
     4681 taaggaatac tccagagaat taccaagaca agaggagtta tatttgatga ttcatattca
     4741 taagatttta ggagaattat gcgaaaatag ttagaatatc ttaatatgct agtaatatta
     4801 taaaaaatta aaaattaact acactttaaa tatgaacaat tgcattactt aagggggttg
     4861 tcttaaaatt gattttttta attaaatttg aagctcaact ccttttttaa acctttatat
     4921 cattacaact ttaatgttta tacgcctgta ctttagatgt atatctactt tttacatttc
     4981 tcaacataca aacctaccat tttttgcagt atgttaattg aaataaacca ttgagttaac
     5041 cctaaattta ttctggactt ataggaatag ttccaaagat actttaaaat attttctgga
     5101 ataattgctt atttcacata tgttttattg tgatgttttg atttaataaa ttcttcaact
     5161 ctgtatatag taaatgtatt atcatgtttg ttttattcaa gcacacttat aatacgcttg
     5221 attttttcac aatgatgaaa tatactaatg ggtgttatag gtataagaat cgaattacac
     5281 ttgaagaagt aagtgataaa tgagatataa atcctcatat gataaactac tatttcattg
     5341 aggaacatat tctaagtgat gtcaagatac tacaatatga ctaaattcct agtgataaaa
     5401 aagtctacga atgagtgtag aaaggggaac taagatgaat aaagttttaa ttatagatga
     5461 tgataaagat ctttgtattt tgttaaaaaa gagtatttca attgaacaga ttgaagcaaa
     5521 ctattgccat tctgggagag atggactggt tgaactttca aaacaagatt atcagttagt
     5581 agttctagac gtgatgatgc caggtatgga tggttttgaa actatggaac aaatacgaaa
     5641 atatagcagt attcccatct taatgctgac atcaaaaagc gataattaca gcaaagtgca
     5701 tggattgcga gctggtgcag atgactatct aaccaaacca tttgaaatta cagaatttac
     5761 tgctcgtgta ctgtctttaa tccgacgtta cacccgtttt aatccaaaaa atgaggaacc
     5821 acagcaactt tctttcaaag gtttgttgat tgaccttgac cagcatacag tgactaccaa
     5881 tggcgaacct atgttgcttc cacccaagga atttggtgtg cttctttact gtgcaaggca
     5941 tcagggtaag attttgacaa aacagcagat ttatgaagct gtgtggggtg agccatatgt
     6001 atatgatgac agcaatatca tggctattat cagtcgccta agaaaaaaaa ttgaactaga
     6061 cccatcatct cccatctatt tgcaaacaat aaaaggaatt gggtatcaat tcaacacgga
     6121 ggtatgaaaa tggatgaaca aacgataatt cttttcttat tcttgctgtc gattggtcta
     6181 ctttttcttt ccatatttgc tttttccaag cttgggcata ttcacaaacg cctaaaagat
     6241 atagaggaga tactggctga tgtacaaatt ggaaacgaaa atcgcaaaat actaattaag
     6301 ccatgtgatg ttatggctcc attggtttat cagcttaatg agattgtcta cgattatgaa
     6361 aataagttac tttctctgaa aaagtcggat aaagctagca aacaacttat gacaagtcta
     6421 tctcatgatg ttcgtacacc attaacaact ctgattggtt atctggatgc tgtacacagt
     6481 ggtatagtta tcggacaaga acgggaggaa taccttgaaa tcgctaggcg tagagcctac
     6541 gacctcaagg attacattga tgttcttttt gactggtttc ggttgaactc tgatgaattt
     6601 acgctgtcaa tagaatctgt ggaaattgca gagttgtcac gcaatatttt gaaagactgg
     6661 attcctatat tccatgaaaa gaaactgaat tttgaaattg atattccaga aaatcgacta
     6721 atggtaaatc tcgacccaga tggatattcc cgtgtggtta ataatctggt tcaaaatgtt
     6781 cttgctcaca gtaaggctcg gcaaatcaag attaccatga gtgaggattc cagaatggta
     6841 ttactccgtg ttgatgatga tggtgttggg attgccagag aaaatttacc acatattttt
     6901 gagcgactgt ataagtgtga taaaggacgt tcagaaaaag gaagtggtct tggtctatct
     6961 attgtcagtc aaatggtgga gcgaatgggt ggtcagattt ccgtagaaag tgagattgga
     7021 aagtataccg tatttacagt gagcttacca ttaatataaa attgtttttc ccccatgtga
     7081 catgtataca cagggggatt ttttgtaagt tataaagttg caaggttaat acaaggttgt
     7141 tgcaaggtta atgtaagatt gaggtggtat gatagcttta ataaacaaag gaggatttta
     7201 ttatttatga ataatttaat tattgaaaca aaaaatctca ctaagcaata tggtgctcaa
     7261 aagagcgttt ctaatttgaa tattcacgtt cagaaaggtc gcatttacgg attgctagga
     7321 agaaacggtg cgggtaagac gacaactatg aaaatgttat tgggtctaac aaaaccaact
     7381 actggagaaa ttaaaatatt tggtcaggac attcacaata acgagaagaa gcttcttcct
     7441 cgaattggat gtttgattga atcaccaggg ttttatccta atctgacagg aacagaaaat
     7501 ctcaaaatct ttgcaaggct tagaggaata ccaaaaaaaa atgcagtaga atcagcactg
     7561 gatgtagtcg gtttgccaaa caatgacaaa aagttatttt ctcaatactc tcttggtatg
     7621 aaacagaggt tggcgattgc tcttgccatt atgcatgacc cacaactgtt gattttggac
     7681 gagcctatca atggacttga tcctattggt attgcagaag taagaaagtt tattcgtgat
     7741 ctctgcacag aacgaggtaa aactattttg atttccagtc acattctttc tgaaattgcc
     7801 ttgttggcag atgacattgg tattattgac catggtattt tgttggagga agaaagtctt
     7861 gcccaattag aagaaaaaaa cagtaaatac atacattttg ctgtatctga tgcagcacat
     7921 gcagcaaggc ttctggaaac ccaacttaac ctgaaaaatt tcaaagtagc agataattac
     7981 aatattatgc tatatgatac agatgtttct gttgcagaaa taaatcgtgc tttcattcta
     8041 aacaacattg aggtatcaga atctcattta tgcgaagata ctcttgaaga ttacttcaag
     8101 aaagtaacag ggggtgaagg aattgcttga tataatttca tgtgaatttt caaaactcaa
     8161 gcggttaaaa tttatactga tttctatttt aggtgcttgc ttatttccaa ttcccgcaac
     8221 aattttgatt gcaaaggaca acttgccctt tgagcaattg ttcaaattag ttgtcaattt
     8281 tggatatttt ttgctattac caattgtatt aagtattgtt gctagccaac tattttttat
     8341 agaaagagat aacgatgtat taaaaaatct tgcaacagtg ccagtgccaa aaggaaaact
     8401 ggctctggca aaattggctg tgttgctgtt tatatcattg ttctactccg ttgcagggtt
     8461 aggagctact atcattggag gtttcattgt tggaatagta gagggggtta ctgtaaaatt
     8521 aggaatgagc attgcttttg gaattatgtt ttttgttact gtacttcctg ttgtggtact
     8581 cattgtatat ttcaatcgca gctatatttt ttctattata ctttcttttg tatatgctat
     8641 tttcaatttt agtatatcct taaatattat caattttgaa ccaaataacc cactattaag
     8701 cgtattacct gctcctgtta taatgcgttg gtggatggca ttttggggag atcccactgg
     8761 cgaatacacg gcacttagac agccatattt gctctcaact cctgcttgtg taggtatttt
     8821 atgtttgata accattatag cagtcctgct gatttgtatg atatacaaaa agcaggagaa
     8881 ttaggaggta tctatgcttg atataattac tacagaattt caaaaaataa aaagatataa
     8941 tattctatgg attggtgttg ttgctgttct gttttcagca ctattggcag tttttcaggt
     9001 tgtaagttca cacggcagtg accctctaac gtatgagagc tttgcaaacg gagttatctg
     9061 gaataatttc agtcttagtt ttccatttgg gattacactt attggaggat atcttattaa
     9121 ccgtgaatat acagaccaaa ccttaaaaaa catcctaact gttcctattt cattacgcaa
     9181 attgctagtg ggaaaattaa ttgcggttgg aggaattgct gtattgttaa gtcttttcag
     9241 cttcttatgc acatttattt tggggttgtt attttgcaat atcaatatat ctgttacact
     9301 cattgtaaaa tccttagttc agattttgtc tgttaatctg tattgttatg ttgctgtttt
     9361 gccgattatt gcttattttg gtcgtaaaca aaatgcattt ctgacgggtg tagggattgc
     9421 atttgtatac gggttttgtg gtgtgtttgt tgctggaaga aatctgacag acttttatcc
     9481 aatcacagca ggtcttggtc tagttggata caacaatggt gctggtactg tttatcagcc
     9541 acttatcggt gctatgactt tgattgtaat tctaatcttg acaactattc tacttgtatt
     9601 tacacctaat tatgataaag ttatgaccat atctaagaaa aaagggataa agcataaaaa
     9661 gtattctcat aaataacata aatattgttt ctattattta agttgctaga cagtgaaaag
     9721 aaaaaatctg tcgtacagtg gctatatgct catgtattca agaagctaca gttttgattt
     9781 ttcataactg tagcttcttt ttgtatttat taaatcacgg ctatcataat acttgcaata
     9841 ggtatagcga caagagagag tactgtcatg tgtgttttat atattacatt aagtatattc
     9901 taaaatatat gttttaaata atatatttga aaataggaat atttaaataa aaaaaataat
     9961 attatatata gttgacaaaa attggcaaat gatataatat ataaatatag aaatagtaat
    10021 cattactatt attaaaaagg gaggaattaa ttatgaactt aaaaggaact aaaacagaaa
    10081 agaatttaat ggcaggattt gcaggagagt cagaagcaag aaataaatat acatactacg
    10141 cttcaaaagc aagaaaagaa ggttataatc aaatagctgc tatatttgaa gaaactgcta
    10201 acaatgaaaa ggaacatgct aaattatggt ttaaactttt acatgatggt atgccttcaa
    10261 cagaggaaaa tttaaaggat gcagcagcag gtgaaaacta tgaatggact gatatgtatg
    10321 caaaatttgc taaagaagct agagaagaag gatttgacaa gatagcttat ttatttgaag
    10381 ctgttggaaa aatagagaaa gagcatgaag aaagatactt aaagctttta gaaaacttaa
    10441 atgaaggaaa aatatttaaa agagatgaag aagttgtttg gcaatgtcaa aactgtggtc
    10501 atgtgtatgt aggtactgaa gcaccagaaa aatgcccagt atgtgaccat ccaaaagcgt
    10561 atttcaatat aaaagctgaa aattattaaa aattatcatt attaaaaagg aatgttaact
    10621 atgaaatttt ctaaacaacg agaactgatt ttaaatgaaa tattaaataa tccggttcat
    10681 cctactgcgg attacctata tgaaaactta aaaaaagata atccaaattt aagcttagga
    10741 actgtttata gaaatttagc tcagctaacg gaacatggct ttataagaaa ggttagtatt
    10801 ccagggtatc cagatagatt tgatggtcga atagataatc actatcatat catatgtgaa
    10861 gtatgtggag aggtatacga tttagaatcc gaagttctta ataatttaca agaattaata
    10921 tctgaggaga cagacataaa aataacatct tataacataa gttttaaagg gatttgtaat
    10981 aattgtaaaa ggtgtagcca agtaggttag ttttcaaatt ctaaaaataa ttaatgaggt
    11041 gagtttatgt gtagtgaaca aaaatttttt atatgtgaga cttgtggaaa ccttgtagga
    11101 atgatacaaa gtggtggtgt acctattttc tgttgtggaa aacctatgaa ggaacttgtt
    11161 cctaatacaa cagatgcagc agttgaaaaa catgtacctg taattgaagt ggatggaaat
    11221 aatgttactg ttaaagtaag tagcacaact catccaatga ctaaagaaca tcatatagca
    11281 tgggtgtatc ttatgactga acaaggtagt caacgcaaat gtctagcagt agatggagaa
    11341 cctgtagtta aatttgcttt gaatgatgat gataaagtga tttctgccta tgcttactgt
    11401 aatttacatg gattatggaa ggctgaactg taatatttaa ataaattgag tcaaagagga
    11461 ggctataaat gtattttcat acaaatagcc tcctataaat ttatataaat tcaagtttta
    11521 attttgttta atgtcaatat aatataagga gaaaataata tgtcaattta taaatgtagt
    11581 gtttgtggat atatttatga tgaatctaag aatgataaaa catgggatga attaagtgaa
    11641 gattgggaat gccctgtatg tacaaaaggt cgtagttatt ttggaaagat aagcactgtt
    11701 tactatgaag aagatgaaaa gatagcagaa gacatagttg aaaatgaatc taaattaaat
    11761 actgaaaaag agggagattt aaattactta agtacatatt taagaagaga tgatgaagtt
    11821 gagaaacata tggatataat tcacgaaatg gctgttacag gaaaatctat aatagaacct
    11881 atgagaacta aactaccagt aatttcttgg gatgatattt taattatggg tgctcaatta
    11941 aatccattac ctttaaatga gcatgatgaa gtaaatacaa caacaataat aggaaaaaaa
    12001 gcaaagaaac ctatgattat agaaaatcca gtttatattt cccacatgtc atttggagct
    12061 ttgtctaaag aactaaaaat agctttagca aagggtgcag cccaaaataa gacagctatg
    12121 tgtagtggtg aaggaggtat attaccagaa gagaaggaag cttcatacaa gtatattttt
    12181 gagtatgtac caaataaata tagtgtaact gaagaaaatc ttaaaaattc tgatgctata
    12241 gagataaaaa ttggtcaagg aacaaaacct ggtatgggag gtcatcttcc tggcgaaaaa
    12301 gtaactgaag aaatagcaaa agtaagaaat aagcctgtag gtcaagatgt aataagtcca
    12361 tcttgttttg aggaaataca atccaaagag gatttaaaga aacttataga tgaacttaga
    12421 gaagtttcag aagggcgtcc aataggtgtt aaaatttcag caggtcatat tgaaaaagat
    12481 atggaattta tagcttatgc taaacctgat tttgtaacta ttgatggtcg tggtggagct
    12541 actggagcaa gtccaaagtt gcttaaagat gcaacttcaa ttccaacaat atttgctctt
    12601 tatcgtgcta gaaaatacat agatacacat ggattagata tagatttggt tataacaggt
    12661 gggcttcgta tatctactga ttttgctaag gcaattgcta tgggggctga tgcagttgct
    12721 attgcaagtt ctgctcttat ggcagcggcg tgtcaacaat atcgtatatg tggttctggt
    12781 aagtgtcctg taggagtagc tactcaggat gaggaattga gaaaaagatt acatatagaa
    12841 aatagtgcta atagagtggc aaacttttta aatgtaagtt tagaagaact taagactttt
    12901 gctagaattt ctggtcataa agatatacat gatttaagtg ttgatgattt atatactgtt
    12961 aactctgaaa tatcaaatta tacaaacata cagcatgtat agattgaagt ttgaaactat
    13021 tagattttag atagctataa aagtaaatga taaatatatt ttataaaaga gagtgtctta
    13081 aaatgaactt tttagttcat aagtcactct tttttgtatg aagccaaata tatttatcat
    13141 tttagcaatg aaaaagaatc ttatctcttt attttgagat agactccttc tatttatgaa
    13201 aattaaagat attaaattgt aaaatattct gtgatgtcgt actattaaac taaagattct
    13261 gtactttaaa gtaaataaaa atttaaggag tgcttattat ggaaagattg aagatacaag
    13321 ttttagttga caataatact tatattgata gatactttgt tggagaacca gcagtaagct
    13381 attatattga gattgatgga aataggattt tgtttgattc gggatattca gatgtattca
    13441 tatcaaacgc tgaaaaatta aatatagatt taggaaattt aactcatgtt gttttttctc
    13501 atggacataa tgaccataca agaggtatcc agtttttaga aaataggtat gatttatcta
    13561 cagttgaact tatttcacat cctaactgtt ttatacctag aaaacatgga gagaaaagca
    13621 taggagctcc attttctgct gaggaaatta aaaatatttt tatgtataat cctaaggata
    13681 agccatttaa tctaagtaag aattgtgtgt ttttaggtga aataccttct ataaataatt
    13741 ttgaaaaaag agctaaaatt ggtaaatgta aaataggaga tttgtgggaa gatgattatg
    13801 ttttagatga ttctgctata gtttgtaaaa ctgataaagg tatttttatt gttacaggat
    13861 gttctcatag tggaatttgt aatatagtag agtatgctaa aagggtatgt ggagatgata
    13921 gagttattgg tatactaggt ggttttcatc tttttgaatt gaataatagg ttagatagta
    13981 caatacaata tttggataaa gaagaaataa gaatgttgta cccttgtcac tgtgtttctt
    14041 ttaaggtaaa agctaagatg aatgaaatat tgaatataaa tgaagttgga gtagggctga
    14101 ccataagtat atagtatttg aaagtatata agatttaagt tgaaggttaa aaagatattt
    14161 tctatataaa gtatcttttt tgttacgttt attttaagag aaatcaatat acttttatat
    14221 gaaatattgt aaaaaaattt atttttaatg agaatatatt ttcattaata aacaatttta
    14281 ttatgtatta ataatgctag aataaatata aataaatcaa agttacttac attaaagtat
    14341 attttttcac aaaatatatt gatttattct tttaaagttt gataattcca atgaatgtaa
    14401 taaaagaag
//
LOCUS       DAEPOZ010000096        14405 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.096, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000096 DAEPOZ010000000
VERSION     DAEPOZ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14405
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.096"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(185..2209)
                     /locus_tag="KR440_002054"
     CDS             complement(185..2209)
                     /locus_tag="KR440_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0699103.1"
                     /translation="MDKKKLILITYDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGEVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLELDEKLIKSPSLMEYMPKSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKRAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCVEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
     gene            2599..3267
                     /locus_tag="KR440_002055"
     CDS             2599..3267
                     /locus_tag="KR440_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699104.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLASNFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            3269..3754
                     /locus_tag="KR440_002056"
     CDS             3269..3754
                     /locus_tag="KR440_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBH0699105.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            3815..4936
                     /locus_tag="KR440_002057"
     CDS             3815..4936
                     /locus_tag="KR440_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HBH0699106.1"
                     /translation="MGTNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGVAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMQFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTAVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            5217..6407
                     /locus_tag="KR440_002058"
     CDS             5217..6407
                     /locus_tag="KR440_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH0699107.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDIVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLVRKGFKH
                     TPLQSLCPEYKDKIITCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     misc_binding    6541..6796
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6855..7856
                     /locus_tag="KR440_002059"
     CDS             6855..7856
                     /locus_tag="KR440_002059"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBH0699108.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            7960..8349
                     /locus_tag="KR440_002060"
     CDS             7960..8349
                     /locus_tag="KR440_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HBH0699109.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIIKKDKHSNIIENVEFSIKDEEE"
     gene            8510..9718
                     /locus_tag="KR440_002061"
     CDS             8510..9718
                     /locus_tag="KR440_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBH0699110.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(9805..10881)
                     /locus_tag="KR440_002062"
     CDS             complement(9805..10881)
                     /locus_tag="KR440_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBH0699111.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            11110..11637
                     /gene="hpt"
                     /locus_tag="KR440_002063"
     CDS             11110..11637
                     /gene="hpt"
                     /locus_tag="KR440_002063"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBH0699112.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            11726..12658
                     /locus_tag="KR440_002064"
     CDS             11726..12658
                     /locus_tag="KR440_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBH0699113.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIVGWMLGIFFTVIF
                     IKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            12720..13946
                     /locus_tag="KR440_002065"
     CDS             12720..13946
                     /locus_tag="KR440_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBH0699114.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTRFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINANTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            14030..14251
                     /locus_tag="KR440_002066"
     CDS             14030..14251
                     /locus_tag="KR440_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBH0699115.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
BASE COUNT         5439 a         1573 c         2443 g         4950 t
ORIGIN      
        1 tatttttctg ttaaatcaaa tatattttta tctttttaac aattaaaaag aagcttatcc
       61 ctatgttgag atagcttctt ttttaatata aataagtttt attattttat aaaaaattta
      121 tagaaaaata aaaaattata attatatata aattttgata aagtaaagat gaattactat
      181 aggtttaaat tatattattt tctattattt ttatgccttt ctcagttatt gatgagcctt
      241 ttcttccttt actagaaatt aagtatccat tgcgtgtcaa atattctagc atatttctta
      301 cttcgtattc tgtgacatta tattccataa atttcattaa ctttgtcctc cccattggtt
      361 tcatgtgcaa tgattttagg atagaaatgc atatgttttt gtcatattgg tttaaatcat
      421 tgaatataga catattactt gacatatgat tattatttaa ttttgaagat atgttttgag
      481 gtaaatcatt tattgtaaga taattggaac ccatgtaagc catatattca acacaatttc
      541 tcagttctct aatattcccc atccaagtgt atgaatttaa agcttctaaa agactcttat
      601 cgagttttat gtttttatag ggaagttctt ttaaaaatga ttctaaaaga agaggtatat
      661 cttctaatct ctttctaaga ggaggtaaat ctaaagtgaa catacttatc ctataaaata
      721 aatccatcct aaatttattt tctataatta atttttctaa atctttattt gtggcagcta
      781 atattctcac atcaattgga ataatacttg taccaccaat ttttctaatt tctttttctt
      841 gtaagactct taaaagttta acctgtaaat catgtggcat atctcctatc tcatctaaaa
      901 atatagtgcc agtatgagct aattcgaata atcctttttt tccacctttt tttgctccag
      961 taaaagaacc atcttcatat ccaaatagtt cactttcaag taattcactt ggtaatgaag
     1021 cacaatttat tgctacaaaa ggtttatttt ttctatttga atgattgtgt attgactgag
     1081 taaaggcttc tttacctgtt cctgtttcac ctgttattaa tataggatta tctgtaagag
     1141 ccattttttt tgccctttta atacaatctt ttataataga actatttcca ataatattat
     1201 taaatttata tttagctaca taacctcttt tgacagaatc acttcttatt ttttgttcta
     1261 atatttctat attgtttaca tcttttaatg taattaagca accctctata tttttataaa
     1321 gataaaatgg ttttttagaa agtatgaatt ttttatctat ttcgtctatg tatataatca
     1381 tattatcgat atttaagttc tttgtaattt tatctaagaa actctttggc atatattcca
     1441 ttaaggaagg tgattttata agtttttcat ctagttccaa catattaagt aaagacttat
     1501 ttacaaaagt aaccttgtta aaggtgttga aaaatattag accatcacct atttcatcaa
     1561 tggtcatgta aagtatagtt tttaatttag atatattatc taatgacgta tttaagaaat
     1621 catgacttag tgtctccttg gacaatttgt atagtttttc tataaatttc tcatttttta
     1681 gttttaatac tactaataat gacatatatg tatctaatga gatttttctc caacctaaat
     1741 caacaatatt ttttatgtat tttggtataa atctaatctg acctggtgtt atagctgtat
     1801 ctaaatcata tatttcatca caatctggat atgtcggtac aaaatctata tgatttatac
     1861 caattttata tagataagta gccatctcaa atgctgaagt tttattggtt gtcataagta
     1921 acgattttgt acctggaggt agtttcatca ttttttcata tccaagtttt gaaagtgctt
     1981 tcgttccatg aataatttca atatcttcct taaaatgttt tattaaaaaa ttactagtta
     2041 aaggtgatag agaaagcact acctctccct ctaaattttc ttttattcca tcaagtatat
     2101 tttgtgtttc tatgattatt tcgtctccaa aaaagtttgt taattcttct ttgtagtggt
     2161 cactatttaa tttatcatat gttataagga ttaacttctt tttatccatt ttatttctcc
     2221 taacaaaata gtatttattt ttaaagtatt taaagtataa ggtaagttat attatacaac
     2281 aaatagtagt ataaatttat atatttgatt aaataatagg tttttttagg attttatcct
     2341 ataaaaacct attttaaaat tatttttgca agataaggaa aatagctttt tatattgtaa
     2401 tgtatatttt aaatagttaa taaaaaaata ttatattttt aaacttgaaa agtaagtaat
     2461 tataagctat ttaatcgctg gaataaattt acaataaaaa aataaataca aaggctttat
     2521 cattaaggta ttggcataag ttttgcttat taaacaagta tactaataaa attcatttaa
     2581 acaggaggga gaatttatat gagtaaatta gaatcaacaa cagaaaaagt tgaggtaaaa
     2641 tggtatgggt atgtagcatt aatattggga gcattatttt tttcaggaat atttaaagat
     2701 gcaccaggac cattaaaagt attggatttt aacaatgtat taggttcttt tggaacattg
     2761 ggaacaatta atgatggtgt aggtacatta gcttcaaact ttagaggtga tggaggtact
     2821 ggccctcgag atggatggct atatgcattg acattaatac catcagttat gtttgcactt
     2881 ggagtagtta gagttataga ccacttagat ggaatgaagg cagctcaaaa gcttcttagt
     2941 ccactattaa agccattatt agggttacct ggatttgcag gactaacttt gatagctagt
     3001 ttacagtcta cagatgcagc tgcatctatg actaaagaac ttaaagatga tgggtatata
     3061 gatgaaaaac aaaaagctgt attttgtgca tttcaatttt caggtgcgtc tgcaattacg
     3121 aatttctttg cttctggagc agcactattt ccatttatag gagatgtacc aatatttata
     3181 ccacttgctt taatattaat tatgaagttt gttggggcga acttacttag attatattta
     3241 aataaatttg acaaggggga agcttaacat gagtgaggta gcaaaaaaag aagtaaaagt
     3301 taaaaaagga aatgtgatag atgcgtttat tggaggagct agaaatggat tccaaataag
     3361 tacaaattca atggctccaa atgtcatctt cggttttgct ataatttctg tgttcaattt
     3421 aacaggtctt ttggatgtaa tagggacaat atttacacct gtaatgagta tctttggact
     3481 tccaggtgtt gcagcaacag ctataatgac aatatttata tcaatgggag gagcatctgg
     3541 agtaatcgca ggtttattta cagcaggtca aataagtcaa gcacatgttg caattctatt
     3601 gccaatgtta ttgctttcag gagcgcagat acaatttatg gggagacttt tgggaacagc
     3661 tgagttaaaa actaaatatt atccacatat gtttgttata gcaactttaa atggttgtat
     3721 tgcaatgctt ataatgaatt atttcgtagt ataggtaatt ataataaaaa tttaatataa
     3781 atggaaagtt taaattttaa ggaggagcta gattatggga actaacaaag attatattga
     3841 gtatgtacat aaagtgtatg attatctaca cactatacca gaaaaaggat ttgaagaata
     3901 taagacatca gagtttgttt taaaagagct tgaaaaaatg gggtttgaaa atattaataa
     3961 aggagttgca ggaactggta ttatagtaac tatagattct aaaaaagaag gaccagtatt
     4021 tgctataaga gcagatatgg atgcattgga atttatcata gatggagaaa agaaaatgat
     4081 acatgcttgt ggtcatgatg gtcatacttc tatgctttta acagctgcaa agttaataaa
     4141 agagagaaat ttagtaaaaa gaggtaagtt aaaaatactt tttcaaccag gtgaagaaaa
     4201 attatatgga gcattaagag taattgattc tggtttgttg gatgatgtag aagaaatggt
     4261 agggatgcat ttaagacctg gacaagattg taggttggga aaggcgatac ctgcaatgta
     4321 ccattgtgct agctgtataa tgcaatttac gattaaaggt atcacttgtc atggttctaa
     4381 accacattta ggagtaaatg cagtagatgc tgtctgttta gcagtgaatg caataaatgc
     4441 aataagagta aatccagcta taccaaattc tataaaagta acaaatatat cagttaaggg
     4501 agaaacttat aataatatac cagatgaagc atttatggct ttagatataa gggcagaaag
     4561 taatgatata atagaagaat taaaagaaaa agcaatacat gctgtaaaaa ctgctgtaag
     4621 ttctataaat gcagaagctg tactaatttc caaagatgga gtaccagcag cagaatttga
     4681 tgaagaactt gtaaacttgg ctgaagaaac tataaaagat gtattgggag ctgatgcatc
     4741 tataggtata tttaaaaatt ctggtggaga agatttccat tattatacac aaaaactaaa
     4801 atgtaaaact acatatattg gattaggagc tgatgctaca ccaggatttc ataatccaaa
     4861 tgtaacattt gatacgaaag ctttagaata tggtgtagat atatggtgta gattggttga
     4921 aaagagattg ggataatatc caaactaata attctacatt taaaattata attatagaag
     4981 ttatagaaaa agattgctta tgttaattag aaaataatta atatgagcaa tcttattttg
     5041 atataatgaa actgattttg gaatattgaa aaaatatgat ggaaaagtaa ttatatttta
     5101 aatctattgg acaaaaaagt gatattatta taatattaag aattgtaaaa taataccaaa
     5161 gtgcatctag taatggactt aacaattata aaaattttat tcttaggagg gaaaaaatga
     5221 gtaagtataa ttttgacaga gtaatagata gagtaggaac agattgtgta aaatgggatt
     5281 ttagaactaa ttgttcaaca aaagcacaaa aagatggttt gcctttttgg attgctgata
     5341 tggattttga atgtgcagaa ccaataatag aagcattacg taaaaaagtt gaacataaaa
     5401 tatttggata tagttcaaat gatagtgata aatactttaa tgcagtttgt gattggtata
     5461 aaaggagatt taactgggaa ataaatagaa aagatattat ttttagtcct ggtgttgtac
     5521 ctgctgttgc tattttagta agaatattaa ctaattctaa tgaaggtgta atagtccaaa
     5581 aaccagtgta ttacccattt gaagctaaga taaagagcaa taatagggac attgtaaaca
     5641 atcctctaat atatgagaat ggtacttata ggatggatta tgatgattta gaagaaaaag
     5701 ctaagtgtag caacaataaa gtactgatac tttgtagtcc tcacaaccct gttggaagag
     5761 tttggagaga agatgaatta aagaaggttg tagaaatatg taaaaaatat gacctatgga
     5821 taatagctga tgagatacat tctgatttag ttagaaaagg atttaaacat actcctttac
     5881 agtcattatg cccagagtac aaggataaaa taattacatg tatagctcca agtaaaacat
     5941 ttaatctggc aggaatgcag ttgtctaata taatagtgaa taatgatgaa ctaaaaaaga
     6001 aatatcaaga agaggttaca gctgttggag ttgcaacttc accaaatccc tttgctattg
     6061 tagctacaat agcagcatat aatgaaagtg aagactggct taatgaatta aatgattact
     6121 tagataataa tatacaattt attgatgaat acttaaaaga aaacctacca aaagtaaaac
     6181 ttgtttatcc agaaggaaca tatctagcat ggttagattt tactgcttat ggattaaatg
     6241 aagtagaact tgaggattta atgtttaaaa aagctaatgt attatttgat gaaggttata
     6301 tatttggaaa agaaggaata ggatttgaaa gaataaatgt agcttgtcca cagtctttat
     6361 taaaagaatg tatggacaga ttaaagacta tatttgaaaa tttataaagt ataggtaaaa
     6421 atttataaag tgtttaaaaa taaaaaaata ataaacttga caaattttaa ctaatcactt
     6481 atcatactat tatgaataaa tagaaaacat aagagagcta aattttattg aattaaactc
     6541 ttaaagactt tgaaaggaag agtagttata gaatgtaatt aaagcgagtt ggtgatggtg
     6601 tgagaccaat atgaagtcta taatgaagaa catcctggag ttgctaaccg aaattttaaa
     6661 agagtaggct tagtcggaac caaaccgtta tcatattggg gtgtatcgag taatatctgt
     6721 acattgtgag ctgggtttga atttatttca aactaatcag ggtggtaccg cggaaaagca
     6781 acctttcgtc ccttttatat agggatgaag ggttttttta atggaaaaat atataaatat
     6841 agggagtgaa tgatatgagc acaataatac caaaggatta taaaagtagt ttgaacgtaa
     6901 ttgatacaca aatagctata aaaaaattaa aagatttttt tgaaatgaga ctaagtaatg
     6961 aattaaatct tataagagtt tcttctccgt tatttgtatt gccagaaaca ggtgcaaatg
     7021 ataatttaaa tggagaaaag gctgtaagct ttgatatacc atttatgaat aaaaatgcag
     7081 aaattgttca atcacttgca aaatggaaaa gattagccct taaaaaatat gggtttgaag
     7141 ttggtagtgg tttatataca gatatgaatg caataagaaa aaatgaagaa ttagacaata
     7201 tacattctct ttatgtagac caatgggatt gggaattggt tatagataaa agtagtagaa
     7261 atgaaaagac tctaaaagat gttgtaaaaa gattgtatgg agtatttaaa gatactgaaa
     7321 tttttgtatg tagtatgtat gaaggtataa aagaaatatt accagaagaa ataactttta
     7381 taacatcaca agagttagaa gatatgtatc cagaattatc accgaaggaa agagaagata
     7441 aaattgtaaa agagaagaaa gcagtattta taactcaaat aggaaaaaca ttaatatcag
     7501 gagaaaaaca tgatggaaga agccctgatt atgatgattg ggaattgaat ggggatatat
     7561 tattttacaa tcctgtttta gattcagcat tagaattatc ttcaatggga attagagttg
     7621 atgaagaatc tcttgataga caattgaaac ttgctggatg tgaagagaga aaagagtttg
     7681 actatcataa gatgctttta aatggcgaac ttccgtatac aattggtgga ggaattggtc
     7741 agtcaagaat atgtatgtat ttcttacaaa aagctcatat aggagaagta caagttggag
     7801 tatggccaca ggatatgatt caaaattgta gtagtgcagg aattgaatta ttataattta
     7861 aatataaact aattgataaa atttatatct aatatgttca tcaaattaat tatatcggtt
     7921 ataatgtaga ataacctata attataagag gtgataaata tggagttttt aaaaataatc
     7981 attaatatag ttttagatat tttaaaaaaa attttggtca gatttaaaaa tgctaaattt
     8041 ggtttggttt ttgtatttga tttacttaaa ctacctgatt ttatgacaga taaaagaatc
     8101 aatatagttg ataaaataaa ggtaatttct gtattaattt ttactatatc ctactttgta
     8161 tctggggttg atataattcc agagatgatt gcaggggcat ttggttttat agatgatgct
     8221 atagttttaa tttggagtat aggtatagtg aatgaagaaa ttaataagta tagagttatt
     8281 ataaaaaaag ataaacattc aaatataatt gaaaatgtag aattttctat aaaagatgaa
     8341 gaagaataac aatttatata tgttattctt ttttttatta acaaaaaatg gttaaattgt
     8401 tgacggagag gtaaaactct attatcataa taagtggata tccaatattt aactaagtat
     8461 tcaatgtgca agaatgctta atttaaaaaa gttatggagg gtaaatatta tgaagaaaaa
     8521 aataggttta gtgcccaaac taatcatcgg tatcatagtc ggtattttaa tcggaagtta
     8581 tgcaccagaa ataatagtac aaatattagt aacagttagt accttattct cagcattcct
     8641 aaaatttgtt ataccattta taattatagg ttttgtaaca gcaggtatag ctgatttagc
     8701 aacaggtgca ggaaagttat tggggataac tacgggaata gcatatggtt ctaccttaat
     8761 agcaggatta ttatcatttg cagtatctac attaatcttc ccaaatttta ttgatgcaag
     8821 tgttgcaagt caaattggag acccagaggc aggaatgtta gctccaatat tcacaattcc
     8881 attagagcct atggtagatg taacagctgc aatcgtattt gcatttgtaa tgggacttgg
     8941 aatatcagct ttaagaaatc atggaaaagg agagacttta tttaatttct tccaagaatt
     9001 tcaaggaatc gtaacgaaaa cgttgtcaac agtgattata cctttattac ctttatacat
     9061 agcaggaact tttgcaaata taactatagc aggggaagta tggacaatat taggggtatt
     9121 ctggaaagta tacttagtag taataccttt acattttgta tatatggcat gtcaattcac
     9181 agcagcaggt gtattttcag gaaaaaatcc agtaagaatg ttaaaaaatc aagtacctgg
     9241 atatttaaca gctataggaa ctcaatcttc agcagcaact ataccagtta atgttggttg
     9301 tgcagaaaaa aatggagtaa gtaagcaaat aagagaattt gttgtacctc tttgtgcaac
     9361 aatacattta tcaggtagta tgatatcaat aacaggattt acaactgcag ttttaatgat
     9421 gaatggtatg ccacatggaa taggtgtatt attcccttat attgcaatgt taggtatagc
     9481 aatggttgca gcaccaggag caccaggtgg ggctataatg agtgcacttc ctttcctacc
     9541 aatggttgga ataccatcag atggtgggct tgcaagttta atgatagctt tatacttaac
     9601 tcaagacagt tttggtacag catgtaatgt atcaggagat aatgcaatag cagtagtagt
     9661 agataaaata aatgatggaa tgagtaaaaa aggaaacaaa gaagagcaaa gtgcttagtt
     9721 ttttaataat caaaaatgag ttatctcaaa tagagatagc tcatttttta tggtataaaa
     9781 aaattataaa ataaagggat ttttttattt aaaaattgct ttaagagatg atattaataa
     9841 tttattttca ttaggtttta atatacatac tctaaagaaa tactcatcta gaccttcaaa
     9901 tgaacaacaa tctcttatta ttattttttg tggaagcagt tgttctcgaa gcgatttagc
     9961 agttaattcc tttgttttga ttttacacaa tatgaaatta cctttagtat tgtatatatc
    10021 taagcttttt atattcttaa gctctttaag caggtaatct ctttcagtgt tcattaagga
    10081 aatagtattt gatataaaat ctaaatcact aaacatgatt tcccccattt tagaagctat
    10141 tatgtttata ttccataaat ctagattttt atttatttca tttttgacat tagtgttaga
    10201 tataagacca tatccaagcc ttattccagg cgttgaaaaa aattttgatg tacctcttat
    10261 aacgaataaa ttagaataat catcaactaa ttgagtacaa gagtatgtat ctgtatcagt
    10321 aaactctaca taagtctcat caatcattaa aaatgagtct gtatttttta aaatttctct
    10381 cacttctact ttagtaaatg caaatccagt tggattacta ggattacaga ttactaccaa
    10441 atcataatct ttagcattga tagtttttat aagattttct aaatttatat gaaaattatc
    10501 ttcttctttt gcaaagtatt tttctataga acaattaatt ttagataact ctttttcata
    10561 ctctgaatag gctggagaga gtaaaagagc ctgtttagga ttaattgtat gtataaatga
    10621 tgaaattagt tctgtagctc cactgccaag tactatatta tctatagaac atttgcagta
    10681 attggatata gaagttttta agtctatgta atcagggtca ggatacatag ataccatatc
    10741 tatattgttt acaatgtatt gtttggctaa acttgatgta ccaaaaggat ttatatttga
    10801 gctaaaatcc ataaattctt cctttgaaaa gccatactta ctagataaac tatataaatt
    10861 agctccatga tttgtgctca taaaagtacc tccaaaaata aaattatata atcgataaaa
    10921 ataactgatt aatatatgta catatagatt ataatacaaa ggaatcgtta ttccaaattt
    10981 ttaatggaaa aagcaaaaaa gtatggataa gctgttaatt taggtctaaa aatgatgaaa
    11041 aagattttaa ttgatatttt ggtatgctat aatatataag ttaatagaag gaaagcggga
    11101 ggatacaaaa tgtacaaagt tacaggaaaa atgttgacag aagaacaaat aaaagaaaaa
    11161 gtttatgaat taggaaaaaa gatagaagaa gactttaaag gcgaagattt actagtggta
    11221 ggtatactta aaggagctag tgtatttgtt tcagatttaa taagatgtat agatttggat
    11281 gtaaatatag attttatgag tgttacaagt tatggaaact caacagagtc ttctggaaca
    11341 gttaaaatat taaaagattt agatgtagat attgaaggaa agaatgtgtt gattgtagag
    11401 gatataatag attctggttt aacattaagt aatctagttg cagctttaaa aactagaaat
    11461 ccaaaatctt taaaactatg tactctactt gacaaacctc agagaagaaa agctaatata
    11521 cctgtagact atgttggatt tgttatagaa gacaaattta ttgttggtta tggaatagac
    11581 tatgctgaaa agtatagaaa tttaccttat ataggtatag ttgaggatgt agaatagatt
    11641 taaatgtgaa tcaaaagcac taatttctaa gggagttagt gctttttagt taattatata
    11701 aaattttagt aaaggagaca tctatatgga tattcagtta aatatattat tatttttcca
    11761 aaatttaaga aatccaattt tgaactttat atttcttgtc tttacaataa gcacagaagc
    11821 accgttatta atattaatta caacaattat atactggtgt ataaataaaa aatgtggaca
    11881 gaaaatactg tttgctataa ttggaaattt tgttgtaaat ttaggcatca aagagtttgt
    11941 aaaagctccc agaccaatag gaataaaagg tcttgaatca ttgagagttt ctactgctgg
    12001 tggttattct tttccaagtg ctcatactca aactgcaaca acattttggg ttagtattat
    12061 gactatattt aaaaagagat atcttcattt aataggtgca gttatggttt taggagtagg
    12121 attgtctagg ctgtatttag ctgttcattg gccaatagat gtgatagtgg gttggatgtt
    12181 aggaatattt tttacagtta tatttataaa aatatttgac tatattgatg ataataaaaa
    12241 ttatatttta cttgtagttt tacttatacc atttataata gtagctatat ttctaaatag
    12301 ccctgaatat tttgaaaaat ttggtataat agtaggattt gtatttggat atatggttga
    12361 ggatagattt gttaaattta atacagataa taataacaaa aaaataaatt ttagcaataa
    12421 aaataagggg agtaaaaata taattttttc aagaatatgt aggtttttgg ttgggatagt
    12481 aacaatagga atgttatata ttggcattaa gttgtttatt tcaatgttaa tagtcacttt
    12541 aaatataagt gactctacta tgagtataat ttttatgaat tttattaaga atacagtagt
    12601 tgttttttat ggaatagcag gagctccagc attatttaaa cttttaaaat taaattgatt
    12661 ttaaggtata gagtcaatag tttaaattaa aaaaattaag ggtagaaaag agtgattatt
    12721 tgaagaaggt agtaataata ggtggtggac cagcagggat gatagccgca tcaactgcat
    12781 gtgagaaagg ctatgatgta acattaattg agaaaaatca taaattagga aaaaaacttg
    12841 caataacagg aaaaggtaga tgtaatatta ctaacgcttg tgaaatagag gaattaatag
    12901 aaaatgtacc tacaaatgga aaatttcttt atagtgcttt ttatactttt acaaatgatg
    12961 atgtaataag tatgtttaat aatcttggag taaagactaa aacagaaaga ggaaaaaggg
    13021 tttttccaga gagtgataaa gcctttgata ttgtaaatgc tcttgaaaga caacttaaaa
    13081 gtaagaaagt aaatatatta ttaaattcaa aagtagaaaa aataatatct aaaaataata
    13141 aaattgagaa agtaatttta aacgataaaa aagaaattaa gtgtgattca gttgtagtag
    13201 caactggtgg attaagttat ccacttactg gttctactgg tgatggatat aaatttgcaa
    13261 tttcacaggg gcacacaatt atagatacaa aaccatcttt aattggtata gaagtacaag
    13321 aaagttttac aaaagattta gaaaaattat cattgagaaa tgttgaaata agggtgttta
    13381 atagcaaaca aaagaaagtt tatagtgatt ttggagaact tgaatttact agatttggtt
    13441 tagatggacc tataatcaag tctgcaagtt gtagaatgaa agatacaagg aaagaaaatt
    13501 acactatatt attagattta aaacctgcat tagatgagga aaaactggat aaaagagttc
    13561 aaaaagattt tcagaagtat acaaataaaa aatttgaaaa agcattagat gatttacttc
    13621 caaaaaaact aatacctata ataataaact taagtgaaat taatgcaaat acggttgttc
    13681 accaaatttc tagagagcag agaaaaaact tagtccattt attaaaaaat ttaaagttta
    13741 cagtaaagag atacagacct attgaagaag ctataataac ttctggtggt gtaaaagtaa
    13801 acgaaataaa ttcaagcaca atggaatcaa aattagtaga aggtttattt tttgctggag
    13861 aagtaataga tatagatgca tatacaggtg gattcaattt gcaaatagct ttttctactg
    13921 gatatttggc aggttttaat tgctaaaaat tacctgaatt aaaaatgttt aaaatcacat
    13981 aatattatca gcaacacaaa ataaattaaa cagaaggaag gtaatcaaca tggcaagtaa
    14041 caataacaac aacagaacag tagtaccagg agcaaaagag gctttaaacc aaatgaaatt
    14101 agaaatagct aatgaacttg gtatgagtaa ttatcaacaa gttgataaag gaaaccttac
    14161 tgcaagagaa aatggttatg taggtggata catgactaaa aaattagttg aaatggctga
    14221 acaacaaatg gctggaaaaa gcaacagata gttataaata attatttata actattggct
    14281 attagtttag ctagagctat ctcaaatata gaaagtaatt tctaaattga gatagctttt
    14341 ttttattaaa ttattaaata gatatatttt tatgtaataa attttatgta tttggataaa
    14401 ataat
//
LOCUS       DAEPOZ010000097        14403 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.097, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000097 DAEPOZ010000000
VERSION     DAEPOZ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14403
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.097"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(79..477)
                     /locus_tag="KR440_002067"
     CDS             complement(79..477)
                     /locus_tag="KR440_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699116.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGGQQKKSKGLFKKTKKSNPHE"
     gene            689..1594
                     /locus_tag="KR440_002068"
     CDS             689..1594
                     /locus_tag="KR440_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699117.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDEEL
                     YNINEQESEYNYNEKNTKTRAKKKR"
     gene            1709..1889
                     /gene="ssrS"
                     /locus_tag="KR440_002069"
     ncRNA           1709..1889
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="KR440_002069"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            2072..3289
                     /gene="cdeC"
                     /locus_tag="KR440_002070"
     CDS             2072..3289
                     /gene="cdeC"
                     /locus_tag="KR440_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HBH0699118.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDECNPCNPCNPCKPNPCNPCKPNPCDDKCECHDKCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSIPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNAECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(3390..4712)
                     /locus_tag="KR440_002071"
     CDS             complement(3390..4712)
                     /locus_tag="KR440_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBH0699119.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(4763..5557)
                     /locus_tag="KR440_002072"
     CDS             complement(4763..5557)
                     /locus_tag="KR440_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBH0699120.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQYEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            5667..7112
                     /locus_tag="KR440_002073"
     CDS             5667..7112
                     /locus_tag="KR440_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBH0699121.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSSNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTNKYKLSKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISVISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            7141..7350
                     /locus_tag="KR440_002074"
     CDS             7141..7350
                     /locus_tag="KR440_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HBH0699122.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            7370..8764
                     /locus_tag="KR440_002075"
     CDS             7370..8764
                     /locus_tag="KR440_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBH0699123.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSRNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTALPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNIPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGFDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVVWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            9004..9756
                     /locus_tag="KR440_002076"
     CDS             9004..9756
                     /locus_tag="KR440_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0699124.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSC
                     HRINAKPSTKIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRI
                     DYMFLLKEELEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDYLKCASNMPLLKV
                     RQSVYTENNDVLYYNEQFIRSDKYKYAVSAEI"
     gene            9782..10195
                     /locus_tag="KR440_002077"
     CDS             9782..10195
                     /locus_tag="KR440_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBH0699125.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDIECEYIILSDLYGASVCTAMLRLTNLHNVKLFSGMNLNMVLELLTR
                     FSDELTVEDIEQLVEESRRGIQSVTLQIKEEEEVF"
     gene            10199..10942
                     /locus_tag="KR440_002078"
     CDS             10199..10942
                     /locus_tag="KR440_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HBH0699126.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLELGDMAIIGSYF
                     KDNYKDFGNVSVEHVKTFMDEIKKIREEL"
     gene            10942..11412
                     /locus_tag="KR440_002079"
     CDS             10942..11412
                     /locus_tag="KR440_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0699127.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDELLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            11429..12187
                     /locus_tag="KR440_002080"
     CDS             11429..12187
                     /locus_tag="KR440_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0699128.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            12171..13013
                     /locus_tag="KR440_002081"
     CDS             12171..13013
                     /locus_tag="KR440_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBH0699129.1"
                     /translation="MTTSSKKLDTISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSSSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGIV"
     gene            complement(13173..14078)
                     /locus_tag="KR440_002082"
     CDS             complement(13173..14078)
                     /locus_tag="KR440_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0699130.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKGVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
BASE COUNT         5618 a         1632 c         2462 g         4691 t
ORIGIN      
        1 ttttatgtca ataatatcaa atttaataat agattaacta aaaataatta tctaattact
       61 taaatagttt atttttatct actcatgagg gttagacttt ttagttttct taaataaacc
      121 tttagatttt ttttgttgac cacctggtgt attttttggt gcaggtgaat ttgatttacc
      181 tttagtaggt ttagccttct tagttttagc tggtttagta tctctttgat tttctgcatc
      241 tgagattttt tccattatat tatctagttt tttgcgttga ttttcatcta aaaatgcttt
      301 gaattttgat actacttcat ctcttccatc taagttctta cctattttta taagttcctc
      361 cataagttca ttttctgatt ttcctttgta actatcagcc atcttttcta ttttatcctt
      421 gtcaatattt ttttgtttag ccattttttc taatgactct gtatctaatt ttttcataaa
      481 aaaacccctt tctataaata tatattgaat tatattcata ctatttatat atagaaataa
      541 tattgaagtg gtgaattgtg agtgatatat aatataaata aaaattctta tagataatat
      601 atatgcactg agctttaaaa atggaacatt aaaatatgaa aaacatactt taatataggg
      661 caattatata atataagggg gaattgtctt gaataactta ctatttttga ttggagtgat
      721 tgcattgagt aatggtaata tctctatatt tgatgataaa aataaatcaa aaggaaataa
      781 tagagttaga aaaaacaaaa aaaatgcaaa tgataaactt tcacagtcta cacatggcaa
      841 tttaaggttt gatgcaaatg atataaaaag aggtttaagg ctcatggatt taagtgaaga
      901 agatttagaa atgggaatgg aaataataac aagaactaaa aaatacatgt caagagatga
      961 aagaaaaata ctaataaaaa tagaaagtgt tttagattta gttagaggaa taaaaaaatt
     1021 aaataatata gatatggtag atacagaaga tgaaacggat tttttcagaa aaatggatag
     1081 tgatgataaa aagaatatga tgataaagga aattatagat gtattcccag aaaaaagaaa
     1141 aaattcagtt gaaaaagcta taggaatgaa aaagaagata gatttatttg cagaattatt
     1201 tttaccagat gactttggtg aaggtggatt tagtttgagc tctttggcaa atataaacaa
     1261 tcttggtagt atgaataatc tcaaattgtt aggaagtctt ttaagaggtg atgatagtaa
     1321 taatgatgat gaagacaata atgatgagtt aaattatgaa gacgaagaat tagattgtga
     1381 agacaaagaa ttggatgatg atgagttaac agatgaaggt gaaatggagg ataatgaaga
     1441 tgaattagta gaagataaga acacaggcta tgaaaatata gaaaatgatg aagatatagt
     1501 atacgatgag gaactataca acattaatga gcaagaatcc gaatacaatt ataatgaaaa
     1561 aaatactaaa actagagcta agaaaaaacg ataattaact aaatacataa agaaaaaagg
     1621 aagaaaaagt aatgatagaa taagattatc tcaattacta tataaaacca ctttaaaaaa
     1681 ttcaaaattt attatataat aataatatga aatcctgaaa catacgataa ggctttttta
     1741 aattcaacct acaaatgaaa tacgggagac cgtgtctggt aaattataac atgcctagtt
     1801 agtttggacg ttaaaggtta ctggcaagtc acccacctgg gagtgcctag ggtgtgaatt
     1861 tacttagcca ccggtgtttc gggacaaaaa aagtgctact atttcaaata gtagtacttt
     1921 ttttattaat atgcattttt ctactaaata cgcctaatta ttgataaatt caaaaagcaa
     1981 gtaacaatta agattataaa agcatagtat taattaggct tcaaaaagcc aaagacttaa
     2041 taagaatcgc ttacagggag ggcaatgaaa aatgcaagat tataaaaaaa ataaaagaag
     2101 aatgatgaat cagccaatgt ctacaatgaa tgaagaagaa gtgtatacag atgaaataaa
     2161 ttcagaagac atgagagggt ttaaaaaatc acaccatcat aatggatgta atactgataa
     2221 taagtgtgag tgccatgatg agtgtaatcc atgtaaccca tgtaatccat gtaaacctaa
     2281 cccatgcaat ccatgcaaac ctaatccatg tgatgacaaa tgtgaatgcc atgacaagtg
     2341 taaatgtgat tgtgaaccat gtgaaatgga ttcagatgaa tgttttgaaa acaaatgtgg
     2401 accagaatgt tgtaatccga tatctccaag aaacttctct gtatcaaatg cagtgccatt
     2461 tgcaatagag gctaatagaa tatttgatac tatgcaattc caaacattta cagatgcaac
     2521 aggtccaaat ggagaaccat taacttttga aacagaagta gtagaagtat ttggttcaat
     2581 tccaagtgca ggtcaagcaa gtgtaactat agaaaaaata tgcttaagta atgatggaat
     2641 agttatagac acaggaatga caactttaga agatttcgat ttagacccat taggagatat
     2701 agtaggaaga aactgtgaaa caacttttga atttgcagtt tgtggagaaa gaaacgctga
     2761 gtgctgtaga caaggaaaag gcaaatcagt agcttataaa caaagaggat taactgtagc
     2821 agttcgtaat ttagtactag agctaagagg tagatgtgga tgtacagagt tcgttgcatt
     2881 agctttccca gcagttagag caggaggtgg atgtaagaga agagttgatt atgtagaatt
     2941 tacttttaac acactttcag caccaatatg cttgccagct gacggaagag ctgttacttt
     3001 aagacaagaa tatcaaacta acttaactgt agattgtata ggaaaatcta tattaaaatt
     3061 agaatgcaac gaatgttgtg aacctttcta tgaattaatt ataccaaatg atatagattt
     3121 agtactttgc ttacaagaaa cagttagcac attaataagt gaacaaatag tagttttagc
     3181 atcaccaaat ccaatccaac caagacttgt tgatactttc tctaaagtat gtgatttttc
     3241 gcaatgtgga cctaatcatg gaagtggaaa gccaagttgc cacagataga taggtaagat
     3301 atatagacag tttaaattat aaataaaaat catctaagga aggctaccaa agtgtataat
     3361 ttacattggt agccttcacg tttttataac tagcgtatag attttagtga tttaaaaagc
     3421 actatagtat gtaatagaca agtaaagatt gatactatcg tcattgtgta tatgtatgca
     3481 taaatattaa gtcctggaat tggaagaaat acatataaag ctattagttg aattaacatg
     3541 ccagtaatag tatttatact tgaagctaat tcttttctta ttgaatgtaa aatgccagat
     3601 agagtttgat ttagggacat aaatagtgga actaaaaaca tagcttttag atattctcct
     3661 gcaagtgtat ttttaaatac taatatacat aaatcttttc caaagaaata aaaaaatatt
     3721 gatgatagta tgcctacaaa taaagtaaga agtatggagt atttgatttt tttaattaca
     3781 ctcttacgct ttcttagagc catttcctgt gaaatagttg gtattaaatt tacaataagc
     3841 gctgaaccaa cagtaaaagg taaaaatata aagggcatta ccatcccact gacaatacca
     3901 tacatactta aagattgttg ataagtcatt cctgataatg ctaatcttga tggtatcatc
     3961 atagaactta tagattgcaa aagtatgttt gacattctat tacaagttat gggaatagac
     4021 atttttaatg tttccataga agaattataa aaatctttta tgcttataat atacctattg
     4081 tcaaatgatg aatctcgcca taagcaaata agcataaata taatattgct caactcacca
     4141 atagatatac ctaatagagc tatataacaa ttcatagatt tattatttat gtacataaca
     4201 agtaaaaaaa caaataatat tttaccaatt tgttcaatga tttgtccaat agcagggatt
     4261 tttacattct ttatgccata gtaatatcct cttagtacgt ttgaaagagt tattataact
     4321 atagcaggac aaacagctag tataaataga tttaatttag catcttttaa aaatttaagt
     4381 gataaaaaag aactattgaa agatgcaact atagatataa ttagtgctac gaaaaatgat
     4441 atatataaag ttgatataaa tagagcatta gtattgtgtc tatcgtttaa agcttttctc
     4501 tttgcaacta gacaactaag tgcagtaggt atacctgttg ttgtaacagc taagcaaatc
     4561 atcaaaaagt taaaaatcat atgatagata cctaaacctg tttcacctag tgctcttgat
     4621 aagaatattt tatataaaaa accaaatatt cttacaataa aattggatat aaataaaata
     4681 actgttgata gtactaaata aggtactttc aaaaaaatca cctccttatc ttttaaatat
     4741 attcacaagg aggtgtaaaa atttataatt ttataaattc tggtttcaat ttattaaatt
     4801 tagatacata tttaaatccc atatttttta gtaatgaata tatgtagtca aactcacatg
     4861 caacttgact tatatgatgt gaatctgaac ctgtggttat gatttcccct cccaagtctt
     4921 tgtacatttg aagtatatac ttagatgggg ttaggtctgg aagattatat ctataacaag
     4981 aggtatttat ctcgatacct ttgccatcat aaatagcttg tttaagagtt tcttcaatta
     5041 tatcagaaaa tagtctatca tctaaaatag tgtcataagg tgcatacctt ttaatcaaat
     5101 ctaaatgacc taagacagag taatctttat aattcttaac aatattatat aaataaagat
     5161 agtaattttc ataaacttca tattgagttt tatctttaaa ataattacca aggtataagt
     5221 ccattgtatc tatagcatgg atagaacata ttataaagtc caatggatat tgatgtgctt
     5281 cctttttgta tgtatccatt aattgagttt gaactccaaa ttcaattcct ttttttattg
     5341 atattttatc tttgtatttg ctttgaagag tctctaaact cttaaaataa tcttcgtaga
     5401 caattgaaaa agaatcatca gcatatacat catagtcaac atggtctgtg aaacaaattt
     5461 cctccaaacc gagttcaata gattttttta ccatttcttc cattgtagat tttccgtctg
     5521 tagagaaact tgagtgcatg tgatagtcgt aaaacataaa tatttcccct ttttataaaa
     5581 attttggtat tatatataca tactaaataa tatcaaatat atataaaagc atcaataaaa
     5641 tataaataca atgagcggtg ataaatttgg aaaaatacag aatagattta aatgagaaaa
     5701 aatcaactaa atatatacag atatttaatc atataaaaga attgataata aataaagagt
     5761 tagtagaaca tgaaaaattg cctccaataa gaaaactatc aaatttttta caagtaaata
     5821 acactactat agttaaggta tacgagcttt tagaaaatga aggatatgta tataaaatta
     5881 tagggagtgg tacttttgta tcaagttcaa attcttataa aagcggtata gataacagga
     5941 aaaataagat tattgtgcaa agagaagatt taattcattt tgacaatggg aatccatcta
     6001 atgatatgtt tcctatcgat tcatttaaaa atgctgtaaa tatggcatta tctaaagatg
     6061 gtagttcaat atttgaatat gatgaaggtc ttggctctga agaattaaga gaaaaaatgg
     6121 tagagtatct aagtgatgga aatataaaaa ctactaagga aaatattcaa ataatatcag
     6181 gtgctcaaca aggtatagat atagtttgta aaggacttat aagttattca gatgttgttt
     6241 ttatggaaga accttcttac aatggagcaa ttgaagtgtt taaaagtaga ggtgcaaaaa
     6301 taatatctat acctatgtta gatgatggaa ttgatatagg tattttaaaa ttaaagttgg
     6361 aaaaaataaa accaagactt atatacatta tgcctaattt tcaaaatcca actggaatat
     6421 cttactcaac atataaaaaa aagaaattga tagaattggc agaagaacat gatttttata
     6481 taatagaaga tgattttata agtgattttg tttttgaatc aaaggacaat agaacaatta
     6541 gaagttatga tgataaaaat agagtagtat atataaaaag cttttcaaaa atacttatgc
     6601 caggtcttag gataggaatt gtagaaatgc caaatgagtt gttaaaaaaa gttttatggg
     6661 ctaaatattc atcagatata tcaacaccag gtttaattca aaaatcaatg tattactata
     6721 tgaataattt tgattggaag aattatttga gtcatataga gaagatatat actaataagt
     6781 ataaattgtc aaaaaacttg attaatgaca aattgagtgg taaattaaaa gttagaaaat
     6841 cttgtggagg aataaacttt tttttagaat taccaagagg atatacatct caagcattta
     6901 caaattttat gttaaacaaa ggagtgtcaa tgcttccagg aacttatttt tttgacaatc
     6961 tagtggatga tagattcttt aggataaata tagctcgccc aagcatggaa gaactagaaa
     7021 agggaatatc tgtaataagt gataatatag atgagttttt ttgtgaatat aaaaacaagt
     7081 tagatataaa aagcaataaa ttattttatt aaataaatag aggtttttta ggaggaaaaa
     7141 atgtttgaca agaagaaatt agataggatt aatgaattag caaagaaaaa taaagaggga
     7201 atcctttctg ctgatgaaat caaagaaaga gaaattttaa ggaaagaata tttagaaaac
     7261 tttagagctc actttagatc aagactagat agtgtcaaag tagtttcacc agaagaatat
     7321 gagcaataca tgaaaaacaa taaaaattag aacttagtag gaggaaaata tgaacttaaa
     7381 gtacgaattt aagaatgctt gggattttga aagaaaagaa aatactattg aacaaattat
     7441 gcaatattct cgcaattata tggagttttt aagtaagtca aagactgaaa gactttcagt
     7501 aaaagaaatt ataaaattgg ctaaagaaaa taactatatt tcaatagatg aagctatgga
     7561 aaaaggaagc ataagttgtg gtgataagat ttatgttata aataaagaga aggcagtagc
     7621 actttttgta ataggtaaaa attatataga aaaaggtatg aagattatag gaagccatat
     7681 agattcacca agattagatt taaaaccaaa tccactgtat caagagagta atctaggttt
     7741 ttttaagact cattattatg gtggaataaa aaaataccag tggacagctc ttccacttgc
     7801 tttacatggt atagttatat taaatgatgg aacaaaagtg gacatatcta ttggggaaga
     7861 agatagtgac ccagtatttt gtgtaactga tttattgatt catttggctg gagaccaaat
     7921 gcaaaagaaa ctatcagaag gaattagtgg agaagcttta aatatattaa ttgggaatat
     7981 tcctttagaa gatgaagaaa aagaacctat aacagcaaat atattaaaga ttttaaatga
     8041 gaaatacaat atagttgaag aagacttatt aagtgctgaa attgaagtag ttccagctgg
     8101 taaggctaga gatttagggt ttgataggtc tatggtgttg ggatatgggc atgatgatag
     8161 ggtatgttca tatgctgctg taaaagctat actagaaact gaacaaccag aatttacttc
     8221 agttgcatta tgtgtagata aagaagaaat tggttctaaa gggaatacag gtatgcactc
     8281 aaagtttttt gaaaatactg tggctgaact gattgcttta gaaggagatt attgtgatat
     8341 taaagtaaga agagctttag caaatagtaa ggtattatca gctgatgttt ctgctggata
     8401 tgacccaaat tttggagaag catatgaaaa gagaaattca gcatatatgg gaaatggagt
     8461 agttttaact aagtatacag gttcaagagg aaagagtgga tgtaatgatg ctaatgctga
     8521 atttatgtca gaagtaaaga gaatttttaa taaaggaaat gtagtatggc aaactgctga
     8581 acttggaaaa gtagaccaag gaggaggcgg aactatagcc catatattgg caaatcaagg
     8641 tgcagaggtt atagactgtg gtgttggagt tttaaatatg catgcaccac atgagattgt
     8701 ctctaaagta gacatatatg aaatgtataa gggatataag gcatttttta atataaattt
     8761 ataagtaaaa atataaatgt aataagtgaa taaaggctgt tgtataaaca atttaatctg
     8821 ttgtacaaca gtcttatttt tatgtaaaaa tagcaatttg tcacactaga gaaaataaaa
     8881 gaaaatactg tcaaattaaa aattatttaa aaaaaaattg acaacgattt catcttgtta
     8941 taatatgtaa taaagaactt gttatataat gttataaaaa tgttacatag ggaggtgagc
     9001 aaaatggatg aaaagagaca aaaaaaagaa atggatttag aagtaaattc ctttgttcct
     9061 ttatatcaac aactatatga caacataaaa aaacaaatag catctggcat atataaacca
     9121 ggagataaac ttccatctga aggagactta tgtaaagaat ttaatataag tcgtataact
     9181 gtgagaaatg cacttaatga acttgtaaaa gaagatatat tatgtaaaaa acgtggtaaa
     9241 gggacttatg taacaatacc agagagaata gaagcaacat gtgcaggaaa tagttttact
     9301 aattcttgtc atcgtattaa tgcaaaacca agtaccaaaa taatctctgt tttaataaaa
     9361 aaagcagata agcaagtagc tgaagcttta agtattgaac tagaagagaa ggtaatatgt
     9421 attaaaagac ttagattaat agatgaagta cctgttattt ttgaagttga ctattttaga
     9481 atagattaca tgtttttatt aaaagaagaa ttagaagata aatctttaat ggaggttata
     9541 tctaataata taagcacatt acctaaacga gtagaaaata tatttgaagt aaaacattca
     9601 aacaaagaat attctgacta tcttaaatgt gcaagtaata tgccactatt gaaagtaaga
     9661 caatcggtgt atacagaaaa taatgatgtc ttatattaca atgagcaatt tataagaagt
     9721 gataagtaca aatatgcagt atcagcagaa atataatcta tatttagtag gaggatgaag
     9781 tatgaaaaag ataatattag catctcatgg agatttttca aaagggcttt taaatgcagt
     9841 aaaaatgata gtaggagatt tagcagattg tgttagttcg tatggtttgt accctggaca
     9901 aagtgcttca gattttgctt tagaattaga aaaaacaata tcagaagata tagaatgtga
     9961 atatataatt ttatctgact tatatggagc aagtgtatgt acagcaatgt taagattaac
    10021 taatctacat aatgttaagt tattttctgg aatgaatttg aatatggtat tagaacttct
    10081 tacaaggttt tctgatgaat taacagttga agatatagaa caattagtgg aagaatctag
    10141 aagaggaata caaagcgtga cattacaaat taaagaagaa gaagaggtat tttagtttat
    10201 gaaaaaaaag ggaatattat cagtatttaa aagtaaaaaa ccaattatag caatgataca
    10261 cttaaaagga gatactccag aagatatctt tgaaagagct aaaaaagaga ttacaatctt
    10321 tgaagaaaat ggtgtagatg gaataatgtt agaaaattac tatggtaatt attatgattt
    10381 agaaagaata cttgaatatg tatcaaaagc taatttgtca ataccatatg gagtaaattg
    10441 tttaaatgtt gatactatgg gatttgaact ggcaacaaaa tataatgcta gttatataca
    10501 ggttgattct gtagtaggtc atgttaaacc tagagacgaa gctactttag aagaattttt
    10561 taaattacaa cgttcaaaat gcccagcgta tttaattgga ggcgtacgtt ttaaatatca
    10621 gccagtatta tcagaaaatg acgttgaaga agatttaaaa ataggaatga caagatgtga
    10681 tgctatagca gttacagaaa atgctacagg tcaagaaaca tcaatggaaa aaatagaact
    10741 gtttcgtaaa aatttaggag actttccatt ggttatagct gctggagtta cattagaaaa
    10801 tgcaaaaaaa cagcttgaat taggagatat ggcaataata ggaagttact ttaaagataa
    10861 ttacaaagat tttgggaatg ttagtgtaga acacgtaaaa acattcatgg atgaaatcaa
    10921 aaaaattagg gaggaattat aatgattaaa ttagtaagag tagaccacag acttatacat
    10981 ggacaagtag catttacatg gacaaagttt ttaagcacag actgtatatt gatagcaagt
    11041 gatgaattat taaaagatga attaagaatg gcagggctta aaatggctaa accatctaat
    11101 gttaagttag taatgaaaag catagcagat tctataaaag cacttaactc aggtgttact
    11161 gataaatata acttattgat actttgtgaa tctgtagaag atgtttatag acttgctaaa
    11221 gaggtaaaag ctattaaatc tataaacctt ggtggaacaa aatcagatga taatcgtgaa
    11281 aatatatcta aagcagtgca tgtatcaaaa gatgatataa agatgattaa agagttagat
    11341 tcagaaggcg taaatgtatt tgtacaatta gttcctgatg atgatgctac aaatgtaatg
    11401 aaattaatat aattttaggg ggatgaaaat ggaatttaca caagttattt tgataactct
    11461 aattgcattc tttgcatata tgcatagttt tgtaggttct acaatgcata atagacctat
    11521 agttgttgca ccacttgttg gtttagcact aggtaatctt catacaggta tagtaatagg
    11581 ttcaacatta gaattagtat ttatgggtgc atttcctgtt ggggcaagta atcctcctga
    11641 ttttgtgtct ggttctatta ttgccacagc gtttgtaata ttaacgggtc aagatgtatc
    11701 agcagcagtt gtacttgcag ttcctatagc aactctagta ttgcttatag ataacttctt
    11761 aatgacagta gtacttacat ggggagctca tattgctgac cgttatgcag aagaggggaa
    11821 tattgaaggc gtagaacgtg tgcaattatt atttggtata ggaaataaat taatattagc
    11881 aataatagta ggtataggat tttcattagg tgttccagta atagaaaaaa tactttcatt
    11941 tataccatca tatgtaactc atggaatgga tgttgcagca ggcgtgattc cagcaatagg
    12001 ttttgccatg ttagctagga tgatgcttaa taagaaaaca gtagcatttt tattattagg
    12061 atttatattg gttgcttacc taaacataac agtaacagga gttgcattgt ttggattagc
    12121 aatagcttta atatatgtta actttgtagg agagaaggag gtaatagtag atgacaacga
    12181 gttctaagaa gttagataca atttcacccg atagtaaaat aacgaggaaa gacttttgga
    12241 aatgctttag aaggtcattg acattagatt catcttggaa ctacgaacgt atgcaaaata
    12301 tagcatatgc atacatgatg gcaccaataa tccgtagatt gtataaagat gataaggaaa
    12361 agaaatctaa ggctttaaaa agacatttag aatttatgtc agttactcct catataagta
    12421 ctcttttggt aggtatatct ggagctatgg aagaagaaaa tgcaaagaat aaagaatttg
    12481 atgctaatag tataaatgct gttaaatcaa gtttaatggg tccagtttct gggataggag
    12541 attcattttt ctggggaaca ttaaagctta tagcagctgg agttggtata gctttagctt
    12601 ctcagggtaa cataatggga ccgattttat tccttttaat aataaatgtt cctcatttta
    12661 ttatacgcta tatatgtcta gataaaggat ttaagtatgg aactcaattc tttaaagatg
    12721 tgagtagttc aagtatagtt tcaaaagtta tggaagcagc ttcaatgtta gggcttatgg
    12781 taattggagg tatgactgca tctaatgtaa tgttaaaact tagtgttaat gtaggtagtg
    12841 gagaatgggc agagcctatt caaacttact tagaccaaat aatgccatgt atgcttccag
    12901 ctatgatatt cggtattatg tattggttat taggaaaaaa ggtaaagaca actacgatat
    12961 taatttcagt tatgattatt tgtatagtat tagctgctat tggaatagta taaataaaaa
    13021 aatcccaagt tcatttgaac gagggatttt ttgactaaat taaataaaaa agaaagatgg
    13081 ttcataataa tttataagca tttatgtgat agttataatt agcttattaa ttagttataa
    13141 acccaaaatt aagcactact ttattaaagt tattatatta taatttcgaa atactcacat
    13201 aatctgttta gaaatttatc ttctaatggg gaaaaagttt tctttttaga gtaaataaag
    13261 aaaaattttc tagtaaaatc aacacccttt attttataac attttatttt acctgcattg
    13321 agataatcaa tagctgaaat gtacgatata aaggagactc caagacctaa tctaaccatt
    13381 tccttaatag cctcatttga ttctacatga gctattacat ttaatttact tactggaaag
    13441 ttatttttac taagtgtgtt tagtattaag tttcttgtac ctgacccttc ttttctcata
    13501 ataaaattta gataagccaa ttctcctata tcgatacaat tatttttatt atcaatttta
    13561 aaatctgaag gagtgataag aacaagttca tcatctaata agtctaggta ctctatttga
    13621 ggattattga ttttagaacc aactaatcca aagcttattc gttcatttaa aatttctgat
    13681 atagcatatt gagaatcata atggcttatg gaaaatttta catctggata actcatagaa
    13741 aaactcttca taaaatcagg taaaatataa gtttcaggta tggaactaca tgctatatct
    13801 ataatacctt ctatttttcc tgaatattct tttatatcat aaattgcacg tttgcaatta
    13861 ttcaatatat atatagcatg ttcataaaga atttcaccag attttgtgag tgtaataact
    13921 ttgttgcttc tatttataag aacagtttcc aactctctct ctaaattctg tatgtgtgca
    13981 cttacagtag gttgagttaa aaaaagttct cttgcggcct ttgaaaaact ctgatgtttt
    14041 gcaacagcaa caaatacttc aagttgttta aaatccatta tttaatcctc cttaaattat
    14101 ctaaaactat tataacatat ttgataaatt aatagtcaaa ggtgattttt aaaatagaaa
    14161 acctttaaat attcatggag tttttgattg tttaggaata atttggtata atattgtaag
    14221 gtacttacaa taaaaatatc gtaaattgaa ttttcaaaat tcaactttaa agtgtttttt
    14281 attacagaaa aattacaatt agcttcttaa tcttattaaa ttcaaataaa tattatataa
    14341 ttattttata atataataaa tataattatt gaaaaaagag ataactgtaa attgagatat
    14401 aga
//
LOCUS       DAEPOZ010000098        14362 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.098, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000098 DAEPOZ010000000
VERSION     DAEPOZ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14362
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.098"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            209..781
                     /locus_tag="KR440_002083"
     CDS             209..781
                     /locus_tag="KR440_002083"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBH0699131.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            845..1495
                     /gene="fsa"
                     /locus_tag="KR440_002084"
     CDS             845..1495
                     /gene="fsa"
                     /locus_tag="KR440_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBH0699132.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGAFLKL"
     gene            1647..4571
                     /locus_tag="KR440_002085"
     CDS             1647..4571
                     /locus_tag="KR440_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0699133.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYGTLIEVKPVAFLNNNLEEELNDIEAEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
     gene            5160..5618
                     /locus_tag="KR440_002086"
     CDS             5160..5618
                     /locus_tag="KR440_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0699134.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFILGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLRTLKSL"
     gene            5679..5954
                     /locus_tag="KR440_002087"
     CDS             5679..5954
                     /locus_tag="KR440_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0699135.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            6013..7287
                     /locus_tag="KR440_002088"
     CDS             6013..7287
                     /locus_tag="KR440_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBH0699136.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            7417..8469
                     /locus_tag="KR440_002089"
     CDS             7417..8469
                     /locus_tag="KR440_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBH0699137.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMDFEDAAMIEPLAVAMHGVLNIGVQVGD
                     AVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDIDPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            8469..9500
                     /locus_tag="KR440_002090"
     CDS             8469..9500
                     /locus_tag="KR440_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBH0699138.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKDIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMASGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            9592..10404
                     /locus_tag="KR440_002091"
     CDS             9592..10404
                     /locus_tag="KR440_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBH0699139.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVNPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            10407..11327
                     /locus_tag="KR440_002092"
     CDS             10407..11327
                     /locus_tag="KR440_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBH0699140.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILASIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            11502..11948
                     /gene="rpiB"
                     /locus_tag="KR440_002093"
     CDS             11502..11948
                     /gene="rpiB"
                     /locus_tag="KR440_002093"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBH0699141.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            11987..12652
                     /locus_tag="KR440_002094"
     CDS             11987..12652
                     /locus_tag="KR440_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBH0699142.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            12762..14264
                     /locus_tag="KR440_002095"
     CDS             12762..14264
                     /locus_tag="KR440_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBH0699143.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTNIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNNSNLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRPYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
BASE COUNT         5827 a         1492 c         2771 g         4272 t
ORIGIN      
        1 tgactacagg agtgaagaag atatagttta tacttatttt aaattatata ttaagttaat
       61 tactttgaat tttaagctcc tgtctatgat atagacagga gcttttttag tattttaata
      121 taaaatactt acaagaatag atagcaaaat tagaacaaat ataatttttt tattaatata
      181 attttagaaa gggctaggtg gaagttcaat gaaggcttta caagaaaaga tattgagaga
      241 aggcagtgtt tcaggaaatg atatactaaa agttgatagt tttttaaacc atcaaattga
      301 tgtagctttt ttaaatgaaa taggaagaga atttaaagag agatttaaag gagaaaaggt
      361 ggataagata tttacaatag aggcttcagg aatagctata gcatcaattg tttcacagta
      421 ttttgataat gcacctgtag tatttgcaaa aaaatcagaa tctaaaaatt tggataaaga
      481 tgtatatgaa actaatgtat attcatttac taaggcaaga gagtattcag ttaaagtttc
      541 taaaaagtac ataaacaaag gcgaaaatat attgatagtg gatgattttt tggcaaatgg
      601 aagagctgct cttggactta aagatttaat tgagcaagca gaagctaact tagttggagt
      661 aggaattgtc attgaaaaag gatttcaagc tggaggagca cttttaaagg ctaatgatgt
      721 aagattagag tctttggcag tagttgaatc tatagataat ggaactgtaa aatttaggta
      781 atttatgata ttatataatt gtgtttagca catattttaa ctatgtaatt atggaggatt
      841 ttttatgagg ttttttatag acacagccaa tattgaagaa ataaaagaag caaatgattt
      901 aggtgtaata tgtggtgtaa ctactaatcc gtctctaata gctaaagaag gtagagattt
      961 tatagaggta gttaaagaaa taagtgaaat agtagatggg ccaataagcg cagaagtaat
     1021 aagtttagaa cataaaggta tgatagagga agcagaaaaa ttatctaaga tacataaaaa
     1081 tatagttata aaaataccta tgacagcaga aggtctaaaa gcggtgaaag tactatcaag
     1141 taaaggtata aagactaatg taacgcttat attttctgct ggacaagctc ttttagctgc
     1201 tagagctgga gctacatatg taagtccttt tgttggtaga ttggatgata tatcacaaaa
     1261 tggtcttgat ttaattgaag aaatcgttga tatatttagt gtaaatagta ttgaggctca
     1321 aataatagtt gctagtgtta gaaatccaat acatgtttta caagctgcta gaatgggagc
     1381 agatatagca acagttcctc ttaaagttat aaaccaaatg attaagcatc ctttgacaga
     1441 caaaggaata gatagcttta tgaaggattg ggaaggtgct tttttaaaat tataattata
     1501 aactacgctt tataagtata ttaaagaggt tattatggaa attctaagta ataaggctag
     1561 gctattttgc ctagtcttat tttggtgtat aatatattga aacatattat tgaaatagat
     1621 taatttttta agtgaggagg cgaaaaatgg ataaaatatt aagagagata caaagagaag
     1681 ataaaaaaaa tccatatact gaccaagaac ttgctgaaat gttaaatgta gctaggtctg
     1741 aagtgatatc agttaggaaa aaaaataata ttttagattc gagagaaaga agaaaaaaaa
     1801 tattaataaa agatatatca aaaattgtaa gtgaaaatcc tcaaatgtct gaaagaaaaa
     1861 taactgaaat attaatagaa aatggatata atatatcgag aagtgcagtt tcaaaattat
     1921 taaaagagga aaatttaaat cagtcaaaga ttgggattaa ggataaagac ccagcatcat
     1981 caggaaagta tgtgaaggtg agtaaagttg tagaagaaga tggatttgaa gaattgatag
     2041 gtataaaggg aagtttgaaa gaaaaggtaa atctagctaa gtcagcaata atgtatcctc
     2101 ctaatggcct tcatactata atatatggag aaacaggagt aggtaaaagt gaattagcta
     2161 catgtatgta taaatatgct ataaaaaaca acattaaaga agagaacagc ccatttatag
     2221 tgtttaactg tgcagactat gcagaaaatc ctaatctatt gatagctcaa ctctttgggg
     2281 tagtaaaagg tgcatataca ggagcagatg caaatagaga aggtcttgta gaacaagcta
     2341 ataatggaat attgttttta gatgaaattc atagattgcc tgctgctggg caagaaatct
     2401 tattttcatt aatagataga ggagaattta gaaggttagg agaaagtagt tctaatagaa
     2461 aggcaaatgt tctcataata agtgctacaa cagaaaatcc agattctaat ttacttcaaa
     2521 catttagaag acgtatacca atggtaataa atttacctaa tatgagtgaa agaccaaaat
     2581 ctgaaagata tgaaataata ataaagtttt ttgaaagaga agcaaagcga gtaaacaaga
     2641 attttataat aactaaagaa gttatgtgtg cacttatgaa ttataagtgt actggaaata
     2701 taggtcagtt aaagagtgat atacaagtaa cttgtgctag agcttttagt aaatcgatat
     2761 tttctagcga taaggtaatt atagatttag atagtttaag ggattatata aaaagtggat
     2821 attatgattg taattgtaaa aatgaaaagt attctgagtt ttcagttgag gatatatata
     2881 tagatattag tgaattggtg agtggaaaaa atagaatttt atctgataat gagataagcg
     2941 agatatataa ttatgcagaa aaagagctta aggttttgga aagtaagtgc ttttcagaag
     3001 aagagttaaa aaatgcattt atagaaaagt tagataagaa gtttaatgct attaaaaaca
     3061 ataaaaattt atctgataga agaagtcgaa tagactgggg aggacaatta aaagagtcta
     3121 cactggagat aatggataag gtaattgtat ttattaagga gcagtttaag catgtaaatc
     3181 aaggtctgta cttggctcta gcaattcata ttgagcatgc tataggtagg ataaaagatg
     3241 gaaaaactat aataaatccg agtttagata aaataaaagc atcaatgcca atggaatatg
     3301 agttgtcaag atatataatg ggtatagtag aagatttgac acaagtaaca tttccagaag
     3361 atgagcttgg atatttggct tattatataa ataaattttg ttataatgag gaaagtataa
     3421 aagataaagt taaagtagta atagttacgc atggtaaagt tggaattgaa atgtctaaag
     3481 ttgtaaatca tattttaggt atagaatgta cactaggtat agaaatagca cttacagatt
     3541 caccatcaga ggggatagag catgtattag aagagcttca aaagatagaa gctagaaaag
     3601 gtattctagt tctaattgat atgggttctt tggtaattct tggtgatgag gttgaaaaaa
     3661 ggcttggaat tagatgtaaa actgtaaata gagtagatac attattggca atggaagcag
     3721 gaaaacttgc aaccattgaa ggaaaaagtt tagatggtat aatagctgat ttaaagaaaa
     3781 ataagaatta tgctatgata aatacaaata aatttagtta cagaaaaaac gagtatggaa
     3841 agaaaaatgt aatcattact ttatgtttga gtggtgtagg tacagcttta aatttaaaag
     3901 agcatataga aaaacaaatt aaggaatatg gcactttaat tgaagtaaag ccagttgctt
     3961 ttttaaataa taatttagaa gaagagttaa atgatattga agctgaatat aatattattg
     4021 ctatctgtgg aactattgat atagattata aaaacgtgcc ttttatttct tataatgagg
     4081 tacttggtaa aaatggtatt aataaagtat tagagcatct aaatagtaaa caaccagctt
     4141 taaatattga aaaccagttg aataatttaa tacatgaaga tttgatttta tacaattttg
     4201 aaggaatttc aaaggagtat ataattgata ctcttgtttc aaaacttgaa gaaggtggat
     4261 atgttgattc aaagtatata ttaagtgtat ataaaagaga agcaatggga agtgttgtaa
     4321 tggcctctaa ggtagccgtg ccacatggac ttccagaaaa cgttataaag cctgcaatag
     4381 caatcgcaag gttgaataaa cctatagtat gggacaataa atttatggtg gatttggtgg
     4441 tactactggc acttaaagaa aataataaga aagaaatacg aagcttattt tcaaagatta
     4501 atgaccagca tactttagaa gttttattaa atactgaaga taaaaatgaa ataaaagaac
     4561 ttttaagcta attattagta ccgagaagaa agacaaaaaa atttgcttta gtataaaatt
     4621 taaaattact caattgctaa gtagttaatt actctatata aggctagttc tatgaaattt
     4681 atttatatga ttttatagaa ttagcttttt tcttggtaaa taaaatatat gaatttattt
     4741 atgtaaaaag taaattttat tggacaattc tatgaaaatt tttcctatat ttcttataaa
     4801 atatagaata tataaaaaat atataaaaat aaattaaatt cataataaat aattttataa
     4861 aatagttgtt aaacataaaa aaccatttgt aaaaaacaaa aaaatcattt ctctaacaaa
     4921 aaaaaccaca aaaaatataa aaaagtatgg ctagttaagt gattaacaag taaaaattaa
     4981 aataaaaatc aaaaaaaatt tatcaatgaa agtcagctaa aatcctggaa gagagcatta
     5041 ttttcgggaa aacattttct tttgtttcac aaaaaaagcc actttttggc acgcaacatg
     5101 ctttatataa agtcaagaaa aactaaggta taagacaaag gcaattagga ggtgtaaata
     5161 tggagtttat aaattttgta agtgataaag ttatagacct taaaatggat tgtaaaacga
     5221 aagaagattt ctttaaagaa atacataata aagtttttga tttaggcttt gtaaaagaag
     5281 aatttggaga aaaaatatta gcaagagaaa atgtatttcc aacaggttta aatttaggtg
     5341 attatggagt agcaatacca catacagatg cagaatatat taaagagcaa tttatatcag
     5401 tctgtacttt taatgaacca gtagtattta gttctatgga agaccaagat gaaaaagtac
     5461 ctgtaaattt ggctttcata ttaggactta atcaaccaca cagtcaatta tctgtattaa
     5521 cagaattaat gggaattatg caaaataaag agttagttga taagttgatg agttctactg
     5581 ataaagaaga agttttaagg acactaaagt ctttataaaa ataaaaaagt actaaaaata
     5641 aaaaatataa aaataaaaat tattaggagg aaataaaaat gaaaaaaata ttagtagcat
     5701 gtggagcagg aatagcaaca tcaacagtag tttgtgacaa ggtagagaga ttagttaagg
     5761 aaaataatat acaagcagaa gttatacaat gcaaaatagc tgaatgtgca acaaagcaag
     5821 aaggagcaga tttaatagta tcaacaacta tactaccaac aacttataat ataccagcaa
     5881 taaaagcaac agcatatata actggagtaa atacagcagc attagataaa aaaatattag
     5941 atgctttaaa ataatcctat catagataca ttgaaataat attttataaa acagaaaaag
     6001 gaggatttat aaatggataa attattagca gtagtacaat acattttaaa tatgggaccg
     6061 acagtaatac taccaataac aattgtagta ctaggaatga tatttagaat taagtttaca
     6121 aaagctttaa agtcaggtct tacaattggt ataggatttg tgggtataaa cttagttgta
     6181 aacctattaa catcaacact agggccagcg gctcaaggaa tggttgaaag attcggactt
     6241 aatttaacag taatagatgt tggttggcca acaacagcat cagcaacatg ggcttcacca
     6301 gttgcaccaa tattaatacc agtttgttta ttagtaaact tactattatt atacttcaat
     6361 aaaacaaaga tattaaacgt tgatatatgg aattattggc actttatagt agcaggtgca
     6421 gttggatata tagtaacagg aagttatgtt tgggctatat tctgtgcaat actaatggag
     6481 ttattagtac tgtttattgc agaaaagaca gcaaaaaatg ttcaggagtt ttatgattta
     6541 gaaggtatag cattaccaac tggttcaaca gcttcatttg ctccacttgg atatttagta
     6601 ggtaaatttg ttgaaaaaat accaggaata aataaaatac aagcagaccc agactctata
     6661 caaaaaagat ttggtatatt tggagaacca atgatgatgg ggattatact aggtataata
     6721 cttggagcac ttgcgggata tgatgtagca ggaatattca atgtaggtat atcaatgggt
     6781 gcagtaatgt tacttatgcc tagaatggtt agaatattaa tggaaggttt aattccaatt
     6841 tcagaatcaa taagagattt cttacaagca aaatatggag acagagaact ttatataggt
     6901 ctggatgcag cagtagcaac gggacatcca gcagttatat caacagctct tatacttgtt
     6961 cctataacgt tgttcttagc agttatacta ccaggaaaca aagttttacc atttggagac
     7021 ttagcgacaa taccatttta tatggcattt atagtgtgct ttagaaaagg taatatagtt
     7081 caatcagtta taacaggaac aatagtaata gctatatcac tttatatggc tacaaacttt
     7141 gcagatgttc atacacaatt attacaacaa gcaaatatga tgccagaagg aacaacaatg
     7201 gttacaagta tagatacagg tggaaacttg ctaaaatggg gaatattgaa attatctcaa
     7261 ttagtttctt catttggaat attctaatat gtagttaata gagattactc ttttttagta
     7321 aagtatgcga aaatttaaca tttagcagct actaaaaaag agtaatattt tttataaatt
     7381 tttaaaaata caaaatacaa gaggaggaga tttatcgtga aagcagcagt attacatggc
     7441 acaaatgata tgagatttga agatatagaa ataaaaccat gtgaaagtga tgaagtaaaa
     7501 ataaaagtta tggcagcagg aatatgtggg tcagatcctc caagagtatt aaaacattgg
     7561 aaatatccag ttccagctat accaggacat gagttttctg gagtaattgc tgaagttggt
     7621 aaggatgtaa aaaatgtaaa agttggagat agagtagttg caattccttt tataccttgt
     7681 aatgaatgtg aatattgtaa aagaggatta ttttcattgt gtgatgacca tggaatgtta
     7741 ggagctaaaa gttttggagc ttttgcagag tatgtaaata taaaagctac aaatgtactt
     7801 ccaataggag atatggactt tgaagatgct gcaatgatag agccactagc agttgctatg
     7861 catggagtac ttaatatagg tgttcaagtt ggagacgctg tagccgtaat gggtagtgga
     7921 acaatggggc agttggtaat acaaggactt aaaatagcag gagcaggaac aattatagca
     7981 gttgatatat ctgataataa attaagagaa tcaaaggaat taggggctga tataataatc
     8041 aatgctaaag atattgaccc agtagaaaaa ataaaagaac ttactggtgg aaaaggcgtt
     8101 gacatagctc ttgaatgtgc aggttcaaaa ataactcaag aacaatgttt acttataact
     8161 aaaaagaaaa gtaagatagg atttttaggg atagcatatt cagatataac tttatcagaa
     8221 gaagcatttg aaaatatatt tagaaaagaa ctagaattaa aaggtttttg gaactcttat
     8281 tctgcaccat ttccaggaca agaatggact aagggaatca atctagttaa tgaaggaaag
     8341 ataaaattaa aagaaatggt atctcataga ttctcacttg aagatactta caaagcattt
     8401 gaaatgataa gagatagaaa agaagaattt aacaaaatat taattttacc acaaggggtt
     8461 gaaaaataat gaaagcagta gttaaaacta aaccaggcta tgataatgta gaagttttag
     8521 aagtagaaga accaaaagct acaggagaca aggttaaaat aaaagtagaa tatagtggta
     8581 tatgtggttc agatatacat tcatttaaag gtgaatatgc aaatataaaa gccccagtaa
     8641 cattaggtca tgaattttct ggaattgttg tagaagtagg agaagatgtt aaagatataa
     8701 aagtaggaga tagagttaca tcagaaacta cttttgcaac ttgtgagaaa tgtatatatt
     8761 gtgaaactaa agattataac ttatgcccaa ctagaaaagg tattggtact caagtaaatg
     8821 gaagttttgc tgagtatgta ctatcaagag aagaaagcat acatgtactt cctgaaaatg
     8881 tatctttatt atcagcagcc cttacagagc cactagcttg ttgtgttcat gcagctcttg
     8941 aaaagactac tatagaaagt tctgatacag ttttaataat aggaccaggg ccaattggtt
     9001 tattattagc tcaagttgtt aaatctcaag gtgctacagt tataatgtct ggtgttacaa
     9061 aagataagga aagattagaa atagctaaaa atcttggagt agatagaact gtaaatgtaa
     9121 tggaagaaag tttggcagat gtagtgaatg aaatgactga tggaaatgga gtaaacaaag
     9181 gatttgattg ttctgggttt atgccagcag ttaacgaagc cttaagactg cttagaaaaa
     9241 aaggtacttt cgtacaagtt ggaatctttg ctaaaaaatt aaatgaaatg gaccaagaag
     9301 caataatcca aagagagtta caatatatag gaagtagatc tcaaaagcca agttcttgga
     9361 ttacagcatt agatttaatg gcaagtggga aagtaaatac agaagcttta gttactagaa
     9421 ctgttggttt agacgaattt aaagaaggta ttacagcttt aatgaacggg gaagaaataa
     9481 aagtagcagt aaaatcttaa aaaaacattt gtattatttt aaaaaaaatt gtaaaatcta
     9541 ctgtaggata aaaaaatgta aggtcttaaa aaataacagg aggaagaaag tatgagcact
     9601 cttagagatc attctaataa tattagaaaa aacataataa aaatggttgc agaagctaaa
     9661 agcggacatc caggtggttc attatcaata gctgatatgt tgacagtatt atattttgat
     9721 aagatgaacg tatgtgcaga aaatccaaaa atggctgata gagatagatt tgttctatca
     9781 aaaggtcatg cagcaccagc tttatatgct acattagcag aaaaaggatt tttcccagaa
     9841 gaagaattaa taacactaag aaaatttggt tctaagttac aaggtcaccc agacatgaaa
     9901 aaagtagcag gaatcgatat gtctactggt tctcttggtc aagggctatc tgcagcaaat
     9961 ggtatggcac ttgcaggaaa attagataac aaagactata cagtatatac tatattaggt
    10021 gatggagaaa tccaagaagg tcaaatatgg gaagctgcaa tgacagcatc acattacaaa
    10081 ttagataatt taattgcatt tgttgactta aatggattac aaatagatgg ttctaatgaa
    10141 gaagtaatga atgtaaatcc agtagatgac aaattcaaat cttttggatg gaatgtaata
    10201 gtaataaatg gacattcttt tgaagaaata ggaaatgcag ttgatgaagc taaaaaagtt
    10261 acaggaaaac caactgtaat aatagctaaa acagtaaaag gtaaaggtgt ttcatttatg
    10321 gaaaataatg ctgcatggca tggaacagct ccaaatgcag aacaagcaga gcaagcattg
    10381 aaagatttag aaggaggtct ataggaatgg gaatagcaac tagagaagct tacggacaag
    10441 tgttaaaaga acttgcagaa aataaagata tagtagtatt agatgcagat ttaggaaaag
    10501 ctacaaaaag tatatcattt aaagaagttg ccccagatag attttttgac atggggatag
    10561 cagaaggtga catgattggt acagcagcag gtcttgctac ttgtggaaaa ataccttttg
    10621 cgagtacttt tgcaatattt gcagcaggaa gaggctatga gcaaataaga aactctgtag
    10681 catatccaaa tttaaatgta aaaatagcag ctactcatgc aggtgtaaca gtaggtgaag
    10741 atggtggtag ccatcaagct atagaggata tatctctaat gagaggtata ccaaatatgg
    10801 ttgtattaaa tccagcagat gctttagaag caagacaagc tattcttgca tctattgatt
    10861 ataatggacc tgtttatata agattaggta gagctgcaac tcctgatgta aacagtgaaa
    10921 attataaatt tgaaatagga aaaggaactg ttttaagaga aggttctgac ataacagtaa
    10981 tagctacagg aataatggta gcaaaagcct tagaagcagc agaagaactt gctaaagaag
    11041 gtataaatgt agaagttgtt aatatatcta caataaaacc attagatgaa gcattaataa
    11101 aagaaactgc taagaaaact ggaaaagtag ttacagcaga ggagcacagt ataataggtg
    11161 gtttaggttc agctgtatgt gaagctttag cagaaactaa agatgtagta gttagaagaa
    11221 ttggtgttaa agatgtattt ggtcaatcag gtacaccagc tgatttatta aaacactatg
    11281 gtttaactac agaagatata gtaaaaaata taaaagagtt gttatagata taaatatatc
    11341 taaataacaa attgatttga cataagtcaa ttaaagctgt cttaaaaata gaaagaaatt
    11401 tctacaatgg gacagcttta tttttacaat actttatttt tatgataatt ataaaatagt
    11461 ctgaaagtaa tcaaaaaaat tataaaaaga ggtgctattt aatgaaaata tctattggat
    11521 gtgaccatgg tggttatgaa ttaaaggaat atataaagga aaatttaata aataaaggta
    11581 tagaagttga agatgtgggt acaaattcaa aagaaagtgt agattttcca atatatgcaa
    11641 aaaaggtaag tcaaaatgtt caaagtaaaa aatctgattt aggcatactt tgttgtggaa
    11701 caggaatagg catgtctatt gcagcaaata agtttaaagg aatcagagca gctgttgttt
    11761 ctgactgttt ttcagctaag gcaactagag aacacaataa tacaaatgta ctttgtttag
    11821 gagaaagagt tattgggaaa ggattagcaa tgagaattgt tgaagaatgg ttaaattcaa
    11881 gttataatgg agaaagacat gcaagaagat tacaaatgat tgatgatata gaaaaagaaa
    11941 atttataaat atatataata tataatttat aacaaggggg agcgttatgt ctattatttg
    12001 tgcttcaata atgtgtgcag accaaatgaa gttaaaagag gaattagaag ctcttgaaga
    12061 agcaggtgta aaattacttc attgtgatgt tatggatgga atatttgtta aaaacttggc
    12121 tatgggacca gagttattaa aatcaataag tgaaaataca acaatacctt tagacataca
    12181 cttagccaca gaaactccag ataaatatat tgatatgatg tcttatataa agccaaaata
    12241 tatttctttt catgtagaat caagtacaaa tgtaaaagct gatatacaaa agttaagaaa
    12301 ttatggtata gggccagtgt tagcaataag ccctcaaaca agtgtggata aaatagaaga
    12361 atatataagt ttagttgagg gtatcttagt aatgacagta aatccagggt ttgcaggtca
    12421 aaagtttaat ctttctgtat tggataagtt ggataaactt acagagatac taaaagatta
    12481 tgataatcct ccatttatag aggttgatgg aaatataaat aaggatacta ttaaacttat
    12541 gaatggtaaa aaagtagata tatatgttgt tggtacttca gctttattta atgataagcc
    12601 tccaatttca tataaagata aaatagaaga actaaaagaa agtataaagt aaccatgtaa
    12661 taaaattata atatgtttct gtttaatagt aaaaatttag gtggaaacaa gaaattttat
    12721 aatatttaat aaaaattttg gttgagtaag gagaattttt tatgggagac ctatataaac
    12781 tacaaaatgg tactgatatt agaggaatag cgtatgaaaa tgattctaaa gaagttaacc
    12841 taactgtaga agaagtaaaa aaaatagcta aagcgtttca tatttggcta aaagaaaaga
    12901 ctaagaaaga taaagtaact gttgccatag gaactgattc taggataaca ggaagccaat
    12961 ttagaagtac tgttattgaa actttaacta atgatgattg taatgtaatt gattgtgaga
    13021 ttgctacaac tccagctatg tttatgacaa ctattatgga tggatacaat tctgatgggg
    13081 cgattatgat tacagcaagt cacttacctt attactataa tggtttaaag tttttcacag
    13141 aaagtggagg tttagaaaaa actaatataa aagagatgtt ggatatagct gttaaaaatg
    13201 atagtgtaga ctatgaggaa gtaaataaaa aaggtgaagt tatagtaaaa aatttaatag
    13261 aagattattc taatttattg atagataaaa ttagaaaagg tgtaaattca agtaaaaatt
    13321 atgaaaagcc tttttctggg cttaagatat tggttgatgc aggaaatgga gctggtggat
    13381 tctttgctga aaaagtttta cacatactgg gagcagatac gacaggcagt cagtttttga
    13441 acccagatgg tatgttccca aatcatatac ctaatcccga aaacaaagaa gcaatggaaa
    13501 gcatatgtaa agctgtgttg gataataata gcaacctagg aataatcttt gatacagatg
    13561 tagacagagc tgctattgta ggtaaaaatg gcaaaccaat aaataaaaat gctcttatag
    13621 ctgttatatc atcaatagta ttagaagaac atccaaaaac agctatagta acagattcga
    13681 ttacttctga aggacttgct aaatttataa atgaattaca aggaagacat catagattta
    13741 aaagaggata taaaaatgta ataaatgaag ctataagatt gaatagtgaa ggtgaagagt
    13801 gccatttagc tatagaaacg tctggacatg ctgctttaaa agaaaattat ttcttagatg
    13861 atggggcata tttaatagct aaaattttaa taaaagtagc aaagctatcc ttggaaggaa
    13921 agactataga agaattgata gaaaaccttg aagaagctca agaagagaaa gaagttagaa
    13981 taggcattaa caaagaagat tttagaccat atgcagagga tatattaaaa gatttagatt
    14041 ctcatgttaa gagtattgat ggatggtctg tagcaccaaa taattttgaa ggaataagag
    14101 ttaattgtga tgaatcaaat ggagatggat ggtttttact tagaatatca ctacatgaac
    14161 cattactagc tttaaatatt gaatcaaata aaattggtgg agcagaacaa atttattcta
    14221 agttaaaact tttcttagaa aaatatgatt taaaaggtct ttagggtaaa tagaaagaat
    14281 atatttatat taaagaacaa atgtaaattt gtacctaaga ttacaaatat tacaaaattg
    14341 taatcttagg taacagagat gt
//
LOCUS       DAEPOZ010000099        14340 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.099, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000099 DAEPOZ010000000
VERSION     DAEPOZ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14340
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.099"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(144..680)
                     /locus_tag="KR440_002096"
     CDS             complement(144..680)
                     /locus_tag="KR440_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBH0699144.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENKDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
     gene            complement(698..2011)
                     /locus_tag="KR440_002097"
     CDS             complement(698..2011)
                     /locus_tag="KR440_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HBH0699145.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEENIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            2347..2787
                     /locus_tag="KR440_002098"
     CDS             2347..2787
                     /locus_tag="KR440_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBH0699146.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLETQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVSLTPKA
                     KELWVTRYTCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(2972..3160)
                     /locus_tag="KR440_002099"
     CDS             complement(2972..3160)
                     /locus_tag="KR440_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699147.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTVIKNSNKKE"
     gene            4221..4616
                     /locus_tag="KR440_002100"
     CDS             4221..4616
                     /locus_tag="KR440_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699148.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNNIKCEEIKDNVIEEKNNTCTTE"
     gene            4659..6758
                     /locus_tag="KR440_002101"
     CDS             4659..6758
                     /locus_tag="KR440_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBH0699149.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYTGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEISVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLKKDATEILVPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGEAMVNQASLTGESLAVNKKEGSYIYAGTGIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNHSIAVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKKDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLREYGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVSGFVRPSTSAFLHNASTVGIS
                     LNSMTNLLENTNTYNYY"
     gene            complement(6983..12193)
                     /locus_tag="KR440_002102"
     CDS             complement(6983..12193)
                     /locus_tag="KR440_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HBH0699150.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRNFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDRQPEKLLDEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKTQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLCIPEGIYSINVSGK
                     DAEKLVGEGSIYDGMNGRNGNNAGTSGDILLSFKEIEKRGELKLYVDGGRGGNGQDGK
                     RGDTGITGEAGENAKIGSKKESKEFAKVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKASLLKGAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSAPIDDSDRNYYYFDRYVFNHASNENQVADDGEKLNALYEKHKDWTISKREDSGLV
                     GAEGKDGRSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILELSHIYEIFHAVEKIEMQLNREILQKYYSFEKKNKAVLSLDDMNIKKEFYKEA
                     KEIYEYLINKLMQSDRIESFIGEKKNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKDELENK
                     MLSVNAKMNLLIQEIIKMKEEEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYQWTNRMVDFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYAEKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSGSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYIDI
                     THGLDVKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNKKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSESIDILKSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIC"
     gene            complement(12208..14331)
                     /locus_tag="KR440_002103"
     CDS             complement(12208..14331)
                     /locus_tag="KR440_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0699151.1"
                     /translation="MQNSVKQEPCDFSHGRFRPEADILDSRLTKVLDLFQQLNMCLHK
                     DNFRGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFEELLKDNGK
                     NIHLCKPLESDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIKEIQNNIVS
                     SLEDYVKAYTEDFISIIGSITMIQEIAKYDEVVKVFKNWLESTTTLTIERFINVLAKQ
                     SGLFLQDSCMELQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEKASKKIMEQ
                     NFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSILTNVTVTQ
                     NEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIRALIPNLVI
                     SSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMDFASEKNIILACNNLDVGSNVKI
                     NVSGKSGELVDSSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNGSKGIDGKN
                     GETGDSGSNGADAGYTSSFHGQMFKGYFAKGTSGKKGDKGGAGGNAGRGGNAGNKGNI
                     YFISDSEEQKNRIINHCDITVKDGEAGPNGRPGSGGYGGSGGYHGYNTLSFSPSGIDK
                     TVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDDGGFNAPNT
                     KLSTAVSKVEENLIKEKYQGYCSEI"
BASE COUNT         4733 a         2606 c         1602 g         5399 t
ORIGIN      
        1 gtataattat aaaaataaaa aaagaatagg atatttttcc tactcttttt tctgtgctta
       61 tatataatat taaagctctt tattttcaat atatatttta aattttctct ttttaatata
      121 tctttttctt ttaataaatc tctttaattt tcttcttcta tatcagagcc aaagggaatc
      181 catgcattac caaactcttt atctttaaat aaataagcta atccagttat ttgcttcaat
      241 ctaagaattt catcaacttc catacccagg tgctttgata tccattcatc agattttcca
      301 atttcacaaa gttcagttat aattttactc attaaatcca cattatgaga acctcttgca
      361 cgattgtgtc gaatagtact tgccattctt tcatcaagag atttatcaat tactgaaaca
      421 ggaagaagtc cgttttctct atcatatata tccttatttt caagcattac tctatatcta
      481 tgaaaaccat ctacaataat atacttatcc ttttcctcat tataataaca gacaattggc
      541 attgtatacc catcttcttt tatactttta tacaacaact gcatttctgg tggggctact
      601 gcatttggat tatagtcgtt agattctatc ttttcgattg gcactgcgat gattccatat
      661 acaggacttt tatattccat agcatcttaa ctcctatcta atgaattttt tcatatttct
      721 tcttaatata ttccgttcta attctctgtt cttttgtagg accaaatccc ataaatctac
      781 acatatggtc attttttaaa atacaataac acattctttt ccaacttggt atatctttgg
      841 taaatttaat atcatccgta tcatcaggta tatcatccaa aaatatgact ctactctttt
      901 tatcttttgt atgatttgaa actccattta attctatttt ataacctttt ctcaacaatt
      961 cacgtataac tatttctgac aaaccaccac ctgtattctg ccaaaactca atagatgttg
     1021 aaaatttttt aatatagcta ttccttatat tttcaggtaa ggtatcaagc aaaaaatatg
     1081 tgaaactttt ccatgtgtgt ccatatggca atttaacact acgatatccc atagcttttg
     1141 tttttccata aatagaagta aaattaactc cttgtactct agcaaccaat ttcacccata
     1201 catctgggtc caatattctg tataaattta aactctcttt tgctgaatca ttaaaaggtg
     1261 atgccactct catttttctt ggagctactc cagctttata gaataagtca taaattttat
     1321 tatagtcata atcaaattta tagtgggcta accatatatc ttctatttcc caatcatata
     1381 taggagatgc cgtccagaca tttttaaatt ccttagttat ccagcacttt cctttatatc
     1441 catgctgttt attcacaata gaacaataac gttgtagaga ttctgctgct cgtgttccta
     1501 ataagcaaac aacttttgca ccttcatgta tatctctaat ccatctacca aattgctttg
     1561 ccaaactttc ttgtctcatt ttatacttat aataagtaaa aggattattt ttttgattta
     1621 ttatgtattc tttgactggc atttctctta cccataattc ttctttctca tcatcccatg
     1681 gataccaata catttcataa tttcctacag cagttcttgt agccattggt aaacacaccc
     1741 aatatggttc aatatcttca atattctctt caaatacttt ttcaacatat tcagtagtca
     1801 aactatattg agcctcaaaa tcttgatgaa atactcctat tttggaactt atattattct
     1861 ttctcttata atccaaaact aaatttagca gtaagccact atcttttcct ccactaaaag
     1921 atacatagat ataatcaaat tcattaaaaa gataagaaag tctttcttga aaagcttcaa
     1981 atacatcgga ttctaaatat ctcttatcca tttgaattat cccctttcaa ttagaagttt
     2041 ataaaacctt acaatacaat attattatgc ttttttagca gtaatcagca taaattatac
     2101 attatttaaa tacacaaatc caactaaaca catataatta taatgtaatt atacttttgt
     2161 attataaact atattttatc attattttac atttttttcc aatatatata aatatcatat
     2221 ttttctatta gctaaaaata attactttat atttttacat gttagtataa taagacataa
     2281 aaacttgaca tactgttgtc ataattactt tgctataatt aaaagtaaaa atggaggtaa
     2341 attataatgt atgaacgtat gcttgataaa agcaataaac cctgtttgtc tcaaataata
     2401 gaatatatag gtaaagatgg atatgcacga ttgtgtaagt tggaaactta cttggaaact
     2461 caatataatt tatcaagaga acttcgattt ccttttggaa acaattatgg ttggggttac
     2521 aaatatagcc ataaatcttt tcatttattt tatgcttttt ttgaaaaagg tgcatttact
     2581 gttctgattc agataggtga caagcaagtt ccagcagtag aaaaaacgtt ggtatctttg
     2641 acaccaaaag ctaaagaact ctgggtcact cgttatactt gtggcaataa tggaggttgg
     2701 attaagtatc gtattttaga agatgcagac ttacttgata ttattcaata tataagagca
     2761 aaaaaatctc cacctaaaca aaattaactt ttattttttg tgatttaaat atagtgtata
     2821 taaattccat tctttccata ttaaaaaaat aatataaaaa atatctatta gtatatctta
     2881 tttagtatgt cttattctac tttttacaga taattaaaaa gttttatcat aaataaatca
     2941 taactaaaca tcctagtaaa tcaaaattca tttattcttt tttattagaa tttttgataa
     3001 cagtccctat acatatgcca attatcattc caaaaattag accataacac attgtgatac
     3061 ccacatttcc tttagaaata cctatagcac aacctatagc cattccaagt atcatgccta
     3121 taaacataca tataataact aaattgttgt ttttattcat agtttttctc cattcaatat
     3181 attttaaatt gcctagctcg ccttcttagc ttaaaacaaa gaaatataaa ttaactttct
     3241 ttcattctaa tatctgcttg tataaatgtc aaacaacaca tttcttgtca taacctcctc
     3301 tttttgaagt aagtacaatc tcccagacac aataatatca atttcctaaa accatttaag
     3361 taacttttat atttacataa tatttacaat cataaactat attgttattc atttatacat
     3421 cataaacact ccctcctaat cattattata cttaaaataa tgttcattta taataatata
     3481 tctaacgcac tttaattgaa aaaactaaaa ctttgccttt cattcccata tataaataag
     3541 tatacttttt gatgctctat tacaaatcaa ctaaattaat tgatataaat tatcattttc
     3601 tgttgaaaat accatttaca catgctatac ttaattgcat aaatggtaat aaatgtcata
     3661 tgattttaca aatctattaa taacaattat tacttttact gtattttaaa gtttttggac
     3721 taaatatcaa gaactttcta aaatacaaag tacaaaaaat ttatataagt tataaattta
     3781 tatgaactag ataagctcaa acaatatgtt atgtataaac tgtgtagtat tcggttataa
     3841 atttatataa attatataag ctatataaag tattacctca actatataag ctagatatat
     3901 aaattacata cataagctag atatataaat tgcatatata agctatataa gaattatttc
     3961 aactatataa actacatata taaattacat acataagcta gatatataaa ctagatatat
     4021 aaactgcata tataagctat ataaaaatta tttcaactat ataaactaca catgtaaaat
     4081 tcatgtaaat ttactaatat aaatcaaata aaattactaa actctctaaa ctaattttgt
     4141 aaaacatact ctatttttaa catttgttac cttatacata agtataaaca aaatctaact
     4201 aaatgaaagg ataatgtaaa atgaatattt taaaaatgaa aaaaacagga atatttttag
     4261 gaggagtact ttttggtact gtaggtgtta agatactttc aagtgactgt gtaaaaaaaa
     4321 cttgcactcg tgcaacagca ggagcattga aagttaaaga ttctgttctt gaaacaacta
     4381 cgaaaattca agaaaatgta gaagatattt tagctgaagc aaaagaaatc aacgagcaag
     4441 aagacttatc tacatctaag gtaaacgttg tacaagatac tcttaaatgc gaacaagaag
     4501 ttcttaaaac tgaaactata aaaactgtag aagatactaa ttctaataac attaaatgtg
     4561 aagaaatcaa agataacgta attgaagaaa aaaataatac ttgtacaaca gaataaattt
     4621 aatgattatt atgattaata atatcaggag gaagtaaatt gaatttttat ataaaacacg
     4681 aaataagagg tcgtatacgc tttgacttac aacttggcaa attaaacaac aagcagtccg
     4741 atactctctt atattattta acttctataa atggtattac caaagcaaaa gtttacgata
     4801 gaactggtga tgcagtcatt tattacactg gtgaaagaga atacataata agagaaatca
     4861 ctaaattcag ttttaacaat agcaaaatag aaaacctagt tcctgaacat accagcaggg
     4921 aattaaacaa ttattataga gacaaattag caaataaact gctagtcaaa agtatctcca
     4981 aaatattttt tccaaagtcc ttaagatttt ttttaattgg acttcattct ataaaatatt
     5041 ttaaaaaagg tctctcttcc ctactaaata aaaaaataga aatttctgtt ttagatgcca
     5101 ctgctatagg tatttctatt tttagaaaag atatcaatac ggctggttca gttatgtttc
     5161 ttcttggtat cggtgagtta ttagaagaat ggactagaaa aaaatctata gacgacttgg
     5221 ctcaaagtat gtcactaaac attgaaaaag tatggttaaa aaaagatgcc actgaaatac
     5281 tagttccaat ttcagagata aaggaaggag atttagtcag tgtaactatg ggaaatatga
     5341 ttccattgga tggagttata gtatcaggag aagcaatggt caatcaggca tcacttacag
     5401 gtgaatccct tgcagttaac aaaaaagaag gctcttacat atatgctgga actggaatag
     5461 agcaaggcaa tattgttatg tgcgtcaagg aaaaagctgg tactactaga ttccagaaaa
     5521 tagtaactat gattgaagaa tctgaaaaac taaaatcttc tgtagagagt aaatttgaac
     5581 atctagcaga tacattagtt ccatacagct ttttaggaag tatattaacc tatgcaatta
     5641 ctagaaaccc tataaaatca ctctctatat tgatggtaga tttctcttgt gcattaaaac
     5701 tctctatccc tatttcagta ctatcagcca tgagagaatg taacaatcac agcattgctg
     5761 taaaaggtgg aaaattcctt gaaggtgttg catgtgcaga tactattgta tttgataaga
     5821 caggcacatt aacaaaagct caaccaactg tttcagatat aattacattc caaaattata
     5881 ataagaagga tatgttaaga cttgcagctt gcttagaaga acacttccca cactccatag
     5941 caaatgcagt agtttatgaa gctgaaaaac aagggcttag tcacaaagaa atgcatacag
     6001 aggtagaata tattattgct catggcattt caacaaaggt cgatgataaa aaggttgtaa
     6061 taggtagcaa acattttgta tttgaagatg agaagtgtac tatcccagac ggtgaggagg
     6121 ataagtacaa caatctatca gatgagtatt ctcacctatt tatggcaata tcgggtaaat
     6181 tatcagcagt tatttgcata aatgaccctt tacgaaaaga agcaaaatat gtaatttcaa
     6241 atttaagaga atatggaata aaaaagattg tgatgatgac tggagatagc gaaaaaacag
     6301 ctaaatctat agcaagtaaa gttggtgttg atgaatatta ttcagaagtc ttaccagaag
     6361 acaaagccaa ttttgttaaa agtgaaaaac taaaaggtcg caaagtaatt atgattggtg
     6421 atggaatcaa tgattctcct gctatttcag aatcagatgt cggaattgca atgagtgaag
     6481 gtgctgaaat agcaagagag atttcagata ttaccatatc agcagataat ttgaataatt
     6541 taatcgtttt aaagcagatt agtaataagc ttatgaaaag agttgattta agttctaagt
     6601 ttattatcgg ctttaattta ggtctgattt tacttggcgt aagtggattt gttagaccaa
     6661 gtacatctgc ctttttacac aacgcatcta ctgttggaat aagtttaaac agtatgacaa
     6721 atttattgga aaacaccaat acttataact actattaatc cccattatat aaaacatatg
     6781 tatataaaaa atttataatt catcttttaa aaagaaactg tcttaaaagg agcttttttc
     6841 taatcaaaat tatataaaac atattttaca aattttaaac tcaacttatt tctatttttt
     6901 acaaaaagga gatgattcaa attttcattt tgaaacatcc ccttttgtaa atttctttaa
     6961 aacttatata ttaattatat tttcaacaaa taattgtatt ttcaaccaat ctttcttcat
     7021 agcattttaa atcattttgc tttagataat taaattcaga ctctacataa ataccacagc
     7081 ctaccaattc aattgtcaaa ttaggtatat tttcctttag tatactagtc tgctcataaa
     7141 taattctttc caataaatca acttgtaata tagaaaaatg cttcataatc tccccatcac
     7201 tatctatatc tttcttgatt tcataccaac tctttgacaa aattcccttc aaatcaatat
     7261 accctagact cttcaatata tctatactct cgctccatgc atacttacta ggtttatcaa
     7321 agtatgattc tagtttcttc ttctgctcat ttgtaagtga atcataagaa agcttcttat
     7381 taaaatgtgc cttcaaagaa atcttagcca tagaatatgg acttctacca gagtttaatc
     7441 ctgcattttt gaacttttca attcctttat cttctactga agaccatatt gagtttattg
     7501 aatctgaatc attgtattta acatcaagac catgtgtaat atctatatag ttttgtggaa
     7561 atacatattg ataatagaca tcttttttat catcatatag ggagaagtat ccactatcat
     7621 ataaatgaat atgcaccttt acttttaaac tatcctctaa aatatttcgt ctaaggttcg
     7681 ggatatttgc atacatggtt acatatttta tggcatcaaa gtttttattt aatggaactt
     7741 caaaactaat ttcatctcct gtcaaaagct ttttcacttc ttcttcatta caatcatata
     7801 gagaatatgt attttcaaat cccacacgtg atttcccatc taaactgcct gttaatgcta
     7861 ccttcaaaat atggttatcc gttttttgcc attggtaagc atcttggtca agaaactctc
     7921 taatctgtct attttgctct ttcgcctgat ttataacctc gttttcatct tctgtaatac
     7981 ctacatggca tctatttaag atatttacta tattgcttcc aaatggaaac gtccatagtt
     8041 gaaaagctgc tgcctcaacc tgacacaaat atctaacctg atttaattca atttgatgga
     8101 taagtttttt ctgatattca ttcatctgat taccactacc tgacatttga taaatcaatt
     8161 ctcccattgc aagattatct tttttcttat caatcttatc agtaatgata atttgagttt
     8221 ccatcatatc ctgtactttt ttaaatatta tagaaatctc ctctgaattt tccattttta
     8281 agcttccaaa ctttgtgctt aattcagtta aaacttcctt catctttaat ttacttatct
     8341 cattttcaaa aatatcttga tttttaactg tactatcgaa tttttctaca aatggtataa
     8401 cagattcttt caaagtttta tctttaaatt ccaccatttg agtattcaat tcatctaatt
     8461 tcttaatatt attttcttta tctttaattg cctctttaac tacctctatt ttactctcaa
     8521 aactagattt tacattaaga caattattaa caccactaat cgcagtattt ataagagaac
     8581 aagcagaact tccaatactc aatgcatttt taattttaga tgtcttttgc tgtctttcaa
     8641 tagcttgttt acttttctct agtgcagcta acttatcttt ttgctgttta ttattgtaat
     8701 ttgcctccaa catacctatt tcttttttct ggatttttgt gtaaatttcc ttatactcgc
     8761 tttcacttaa cccctctttt gctctttgaa tatcgtcagc tttttctgca tagttatcat
     8821 catctataga cttaactata atctccttaa tttggtcaaa tttagttctt ctttcttcat
     8881 caacacctgc caaattataa gtcattttat ctaaagcatt ttttctatct tcacttagct
     8941 gttctccaag tacaaatctc ataccatcag aatccaattt aaaatctgaa aaatcaacca
     9001 tcctgttagt ccattggtat tcctcttctt tagctgaaag attttccttc aaagattttg
     9061 cactgtcaaa tgcccctaga caagcttgac caacaccact aaatgcagtc agtaaactca
     9121 atgcgctttg tattccagaa agaattaact gtgttttcat ttgttcctca agttttgcct
     9181 gtttcttttt tagttcctct atgtcttctt gctcctcttc tttcatcttt ataatttctt
     9241 gaatcaacag gttcatcttt gcattaacag aaagcatttt attctcaagt tcatctttta
     9301 aaacttctat ttgttgattt ccagattcaa tttgagttag gatactttcc ttataagtat
     9361 tgaaatatcc ttcttttaac tcatctttct ttccttgttc caattctctt aaggtcttgt
     9421 ccagataatc tgtcaaagaa cgaatatctg taacacgatt tcctccaatt tcactttctt
     9481 caatcatctg cacatagaat gctgtttttt gttcaagtgc aaataaaatc atactccatt
     9541 cgctaaaatt cttcttttct cctataaaag attctattct atcagactgc atcaatttat
     9601 ttataaggta ttcatagatt tcctttgcct ccttataaaa ttctttcttt atattcatat
     9661 catccagaga aagcactgct ttatttttct tctcaaaact atagtacttc tgaagtattt
     9721 ctctatttaa ttgcatctct attttctcaa ctgcatgaaa aatttcataa atatgtgaaa
     9781 gttctaaaat atcagcttct aaaattccca ctgcacacaa cagtctaaga tttctataac
     9841 ctttaattcc agaaaaacca aatctctcga aaaattcata gaagaaggta tctattgaca
     9901 aagaatactc ctgctttaac aattcctttt cttttaatgc atctccagag cgaccgtctt
     9961 ttccctctgc tccgactaaa cctgaatctt cacgtttgct aattgtccag tccttatgtt
    10021 tttcataaag agcatttaat ttctctccat catctgctac ttggttctca tttgaagcat
    10081 gattaaacac atatctatca aaataatagt aatttctatc actgtcatct attggagcag
    10141 aggtacctat atatcttatt gtttcttcat atatacctct aattccacca agacctcctg
    10201 caccaccagc tcctccagag cctccttttc ctgctagacc atgatttcca tcttttttac
    10261 ttatagcacc ttttaataaa gaagcttttc caaggagata aacctgtcca gacttgctcc
    10321 caagacctgc atttccacca ttacctcctg tgcctccagt tccaccttgt tttccttttt
    10381 ctgcttttac cacatagtat tttgtacctt taaccttagc aaattctttt gactcttttt
    10441 tagaacctat tttagcattt tccccagctt ctccagttat ccctgtatct cctctttttc
    10501 catcttgtcc atttccacca cgtccaccat ctacgtaaag tttaagttct cctctttttt
    10561 ctatttcttt aaaagataat aaaatatcac ctgaagtacc tgcattattg ccatttcttc
    10621 cattcattcc gtcgtaaata gaaccttctc caaccaattt ttctgcatcc ttaccagata
    10681 cgttgattga ataaatccct tctggtatac ataatttttc tgtaagtagt acaatattct
    10741 tacctgagaa gacttctttc tccatattac agtcaatttt caactcacaa tctccataca
    10801 tatatataga tgacaaaata ttaagttgat aaccagatat tattttagtc aaatccgata
    10861 aattcacttt aggtatagga atatatatag aaagtacatt ttctttctca tatgatttta
    10921 tatatgtaca actttgaatc ttctctatcc tatactctgc atcttcaagc tttttggctt
    10981 cttcttccct atattcttct tctgctatcc aatatttgat atcttttaat gtatcttcta
    11041 aagtattttt tagcaatgat tttaacaact cacttagacc aacaatatga gtctcactac
    11101 agtagctatt taactctaaa agaagacgtg tttcttctaa tatattcact ttctgtacgc
    11161 catcaccata tgaaagttct tgtacaaact ccataagctc tttcatatta ttagtcctac
    11221 tacataaatc tgtatacatc ttatctaatt gagtcttttt atcctttaga ctatttatag
    11281 aagatagaat ggaaatagaa tcttccatac cttttttgac actttctata taaggaacta
    11341 aaagtttttc taggtcttta taggttacat caacggcttt ttgtatttct attttacaag
    11401 aagcagtaag catcaatccc aaatctgttt tttcaaatgt ttcaggtaga tttttcattt
    11461 catccaacaa cttttctggc tgtctatcta gtggattaca taatttaata gatacaccat
    11521 ctgataatgc tacatttaat aagtacacta attccttaca accgtcgtaa tctttctctt
    11581 gactatctag gtcttctttt gctttttcca atatattcct tattttttct tctgttacat
    11641 taccgattag tgttttaggt attttattga taagaaagaa gaaattttct ttatactttg
    11701 cataatcacc aagcatatct ttcaaatttt tgcctgtttc tatcaataat gtatgtctag
    11761 agtccagtaa ggattgatag tctaaaataa ctaccatccc tttcaccttt gaagatgtag
    11821 aaatagtttc ccttacaagc atagcaccag caatcatata ctctgctcca cggttatcaa
    11881 aaaaacctgg gcaatcacat aatgaaaaat ttctatttgc aatatctaca acttctggac
    11941 ataaagtctt agaatataca ccatttccaa tttcgacagc tgaatctttt gcagttatat
    12001 atatttgtcc agtctcttca tccgtttttt cttccatcac gcaacctttt aaataattta
    12061 tacaagtact ctttccaacg cctgaagcac ctaagaataa caaaattgac ttttctttta
    12121 agtcatattt tctaagctcg tctcttcctt cttccaaaag gtttacgatt tcactataaa
    12181 gtttatttgc catacatcca tccctttcta tatttcggaa caatagcctt gatatttttc
    12241 tttaataaga ttctcttcta cctttgaaac agctgttgat aacttagtat ttggagcatt
    12301 aaatcctccg tcgtctcctc tatctccata ttcagcgtat tctcttgctg gtcctgtttt
    12361 gccttcattc caacttccgc cactaaattc tatatgatat ttaattatac tatctaactt
    12421 ataatcccaa cttgtaaaat atcctgtata gtaaacagtc ttatctatac cacttggaga
    12481 aaatgaaagt gtattatatc cgtgatagcc accacttcct ccatatccac ctgaacctgg
    12541 tctaccattt ggacctgctt ctccatcttt cactgttata tcacaatgat taattattct
    12601 atttttttgt tcctctgagt ctgaaatgaa gtaaatatta cctttattac cagcatttcc
    12661 acctcttccg gcatttccac cagctccacc tttgtcacct tttttaccac ttgtaccttt
    12721 tgcaaaatac cctttaaaca tctgtccatg gaaactactt gtatatcctg catcagctcc
    12781 attacttcca gaatctcctg tttcaccatt tttaccatct attcctttag aaccatttcc
    12841 tccatttgca atcagtgtta aacgataatt tctaatttct tctttaatat ataagtaaat
    12901 attacctgca tctctaccat cttcaccatt tttcccatac atagaactat ctacaagttc
    12961 tccgctcttt ccacttacat taatttttac attactacct acatcaagat tgttacaagc
    13021 aagaataata tttttctctg atgcaaagtc cattccaagg ttatcatcca tataaatagt
    13081 atctttggca aagaacataa atgtagaaac attgtcatct acttttctat ttttgaaata
    13141 ttccaataca ctactaatta ctaagtttgg aattaatgct cttatttcaa tacctttctt
    13201 atcattaata ttaattgttt ttactataaa tggatttaaa atgtatttac caataaattc
    13261 tctaattatc tcattacctt cttctccata tgcacttaat cttgttatac tgtcttctaa
    13321 ctcttcattt tgtgtaactg taacatttgt caaaattgaa ttaaaatcac ttacactata
    13381 tacataatcc tttttatttg caacttccat taatttttct ctatttggta atgtaaatac
    13441 ctgtttttgt acttcataag aaactaaaac tttttctaaa ttctttctta ttataatgaa
    13501 gttttgttcc attatcttct tacttgcctt ttctataata aattccatat gtacagaagg
    13561 taataaaatt tccccagttc catgttctgt atatttactt aaagcatcaa ttatatctaa
    13621 gtttcctttt atcttatttt gtaattccat gcaactatct tgtaagaata agccactttg
    13681 tttggctaat acgttgataa atctctctat agtaagagtt gttgtacttt ccaaccagtt
    13741 tttaaatact tttacaacct catcatattt tgctatttct tgaatcattg ttatacttcc
    13801 aataatactg ataaaatctt ctgtatacgc cttaacataa tcttctaggc tagatacgat
    13861 attgttttga atttctttta tcaacgtatc taaaactaat cttgcatctg cactaattgg
    13921 atattggaat gctgatgtct tatctttaac ttcatctaat tctagtactt gctttaataa
    13981 gccttccctc tcgtctgatt caagtggctt acaaagatga atatttttac cattatcttt
    14041 tagcaattct tcaaataacc aagctttttc ctctatatta ctatctaaat tttcacataa
    14101 ttgttcatat tttcttttta taacattata tatttgtttt tctgttcttc ctaagaaact
    14161 ttttgaaatg aaaaaagtta tactgcccct aaaattgtct ttatgaagac acatatttaa
    14221 ctgttgaaac aggtctagta cttttgtaag tctagaatct aatatatctg cttctggtct
    14281 gaacctccca tgactaaagt cacagggttc ctgcttcaca gaattttgca tactaaaata
//
LOCUS       DAEPOZ010000100        14297 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.100, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000100 DAEPOZ010000000
VERSION     DAEPOZ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14297
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.100"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(236..1117)
                     /locus_tag="KR440_002104"
     CDS             complement(236..1117)
                     /locus_tag="KR440_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HBH0699152.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSNKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
     gene            1573..2547
                     /locus_tag="KR440_002105"
     CDS             1573..2547
                     /locus_tag="KR440_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699153.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYKLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNHPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            complement(2663..3694)
                     /locus_tag="KR440_002106"
     CDS             complement(2663..3694)
                     /locus_tag="KR440_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBH0699154.1"
                     /translation="MKKIRKLATKLLITTIVLVSGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNNKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPTVGAVAQTQKPALPGKHTMLPHQDGAK
                     VVGKFGDIKDKHVMYEGVRCVVINSSVNTVTFLGSKSNLDPSNLTIAVIGFMNTPYGM
                     FALAPEGEPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIEITDEKANIGL
                     GQIMYIRYE"
     gene            complement(4306..5157)
                     /locus_tag="KR440_002107"
     CDS             complement(4306..5157)
                     /locus_tag="KR440_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH0699155.1"
                     /translation="MSCILKLKQIFEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            complement(5174..6094)
                     /locus_tag="KR440_002108"
     CDS             complement(5174..6094)
                     /locus_tag="KR440_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HBH0699156.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDGIQGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            complement(6204..7118)
                     /gene="murQ"
                     /locus_tag="KR440_002109"
     CDS             complement(6204..7118)
                     /gene="murQ"
                     /locus_tag="KR440_002109"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBH0699157.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNRVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            complement(7217..8314)
                     /locus_tag="KR440_002110"
     CDS             complement(7217..8314)
                     /locus_tag="KR440_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBH0699158.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLTFFKKTKADGIRLDMGFT
                     GSEKALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLTGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFDFKPSTK"
     gene            complement(8547..9893)
                     /locus_tag="KR440_002111"
     CDS             complement(8547..9893)
                     /locus_tag="KR440_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0699159.1"
                     /translation="MLDKFTEFLDSKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            complement(9982..10290)
                     /locus_tag="KR440_002112"
     CDS             complement(9982..10290)
                     /locus_tag="KR440_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0699160.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFENE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEASVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            complement(10551..10940)
                     /locus_tag="KR440_002113"
     CDS             complement(10551..10940)
                     /locus_tag="KR440_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699161.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSNKNTKKEMEKNQRTDYNIPTISFK
                     SGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRGFSL
                     GFFRLYILFVLSFAIIGYFWIYKGIHI"
     gene            complement(10942..12102)
                     /locus_tag="KR440_002114"
     CDS             complement(10942..12102)
                     /locus_tag="KR440_002114"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBH0699162.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEI
                     PKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAIIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDKNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGVHNLTLIGYIKELLLEVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            complement(12532..13551)
                     /locus_tag="KR440_002115"
     CDS             complement(12532..13551)
                     /locus_tag="KR440_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0699163.1"
                     /translation="MLVKYLKGRSKYIALPLVVVSIINIYLFAIDILKNRYSELIYLD
                     FLVLFIIMVFFVIDYINFRNSYINLYNCIENSGEIDSYLIDGQSFEENLIKDIIENLK
                     IKNNSDIETYKQSLKELDDYIAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKI
                     NFLVNSILYSSRSNTMFEDVFINKFNLEKLVKMSIKNNSFLLIKNNVEVSLNELENDV
                     YTDSKCMSYVLDQIINNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRV
                     FDKGFTGKNGRNQLYKSTGMGMYFVKKMIDSLGHEIEVCSKIGSYTIFNIYFYDISDY
                     LSLDS"
     gene            complement(13551..14225)
                     /locus_tag="KR440_002116"
     CDS             complement(13551..14225)
                     /locus_tag="KR440_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0699164.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFMDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIDILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GIGYII"
BASE COUNT         4636 a         2523 c         1523 g         5615 t
ORIGIN      
        1 taaaaaacaa taaaattaag ttataaacac caatatattt ctattatttc aataagtata
       61 caattagatt agaatctaac gctagtaata aaaaattact ttcccaaaat agatataagc
      121 ctcattttat cgttcaaata atgaaaatat atttgatttc atgcaaaaag agtgcctaat
      181 gaactaaaaa gttcattttg agacactcat ttctttatat aaatttatat agtttttaat
      241 cgttcgcatt caaccactca gttaagactt ttatatattt ctcattttct tcaacgaatg
      301 gcatatgtct tgaatactca aataactccc acttactatt aggtattcta tcatacatag
      361 tctttgcaat atatggcgaa cataaatcta ttgctccact agttactaga caaggctctt
      421 ttatttcatg taatctatct gtaaattcat atccagaaag agttcctgta ggagaaaatt
      481 cattttgtcc ccatcctaca atatatgctt cagaacctga ttttttaggt cttctcaggc
      541 actctggaga atcttctgtc acttcacctg cacaatacat ttccataaat ctttctaatg
      601 catcattata ttctttatta gagtaatctc cagtatttac tgcatcaagc aatgcttttt
      661 ggtcaacctc actcatatat gatattcttc ttttttgttc tttctcccaa agttttgcag
      721 aagaaagagt agatgaaagg atgtaagact taataccttt tggtttatat tcaatagcat
      781 accaaatagc ctgcatacct ccccaagatt gacctaacag atgaatttca tcaagtccta
      841 gatgttttct tagttcaata agctcctcta tccaagtatc tgcattaaat aactcaggat
      901 gcccttctac aaaggaattt ccacaaccaa tttggtcata cattattact tgtctaccac
      961 tttcagctat cttatccaat acctcaaaat agttgtgtgt agaacctgga cctccatgaa
     1021 gtaatactaa tggcttcttc ccttccgtat tttctcctac tatacggtaa tacgttttaa
     1081 aacctttaaa aggcatatat ccttcagtaa ttttcatatt tatctcctcc aatttgtaat
     1141 tataaacaat aatcaagttc attaacaata ctatttaaaa cctctcatac gtatttaatt
     1201 atgtctttta atctttatac ctacttttat aaatagtata acataaaagt taaaaatctc
     1261 tatactaaac caattataaa attatattaa ctatataatt ttatttttta ataatagtat
     1321 atacatattt gattattttc atttgctatt tagaatcaat tttaagtttt cttcttataa
     1381 taaacttcct ctttgtggtc tcatgttagt ctctaaattt aaaattttac aaaaaatttt
     1441 cataaaataa aattatattg attgtatcta aacaaatagg gcaataaaat aattaagtat
     1501 agttacttac ctttcaagca atattagttt tatgttagaa ttttattata ttgatttaca
     1561 ggaggtttgc atttgaataa tattaattca cttaaagtaa taaaattatc tcttggatgt
     1621 tctcttgcta tattcattgc ttggctatta aaattagaat attcaatggt tgctggagtt
     1681 atagtactac ttacagtaaa ggacactaaa agagaaactc ttaaaggctc cattggaaag
     1741 atttatggtt ttcttttatg tactattttt tcatatttat gttttaatat tttaggatat
     1801 aatttgactt cattttcaat atttatattt ataattatac cactttgttt tttgttaaat
     1861 attcaagatg ttattgctat gtgtgtagtc atagcatccc attactacct tcaaggagaa
     1921 acttctatca aatggatttt aaatgaagct ggaatatttg cagtaggaac tggaataggt
     1981 gtcttaataa atatgtatat tccaacaaat atacataaaa tccatgaagt tcaaaaaaag
     2041 ttacaggaag aagtgagtat tgtacttata gacattgcag atattattgt aaatcctaaa
     2101 aaagaaaatg gatatagtag agatttatat aaattaaatt cacttattga cagcagtatt
     2161 tctgaaactt acgataatat taataatact ttattaagtg atactagatt cttcttagaa
     2221 catatggata ttataaaaag ccaacgggat atcttagaaa atttgtatag ttatgtatct
     2281 caactaaatt ctacaccacc acaagctcac attctatctg ctttcataca taaaataggt
     2341 tatactgaat ttgaagctga aacaggcaat cttcttcttg aagaattgaa gagattaatg
     2401 cttagtatga aaaaccatcc tctacctgtt gacagaacag agtttgaaaa cagagctatt
     2461 ttattcctat gcttgactga gttaaaacaa tttttagtta atcgtaaaca tgctcagatg
     2521 cttagagata ataattttta taaataaaaa ggtgagaaat ccagcagttg acctgactac
     2581 aaaatatcag atataatatt aaatgcgccc ctagtaagag ggcgcgatgt ttataactta
     2641 ttctactgtc atgattaaat cattattcat atcgaatata cattatttga ccaagtccaa
     2701 tattagcttt ttcatcagtg atttctattt taatagtcac atcatttttt ttacagtact
     2761 ttgctaattc tgcaccagaa cgatttttac cactaaaatc taaagaaaat ggcttatcat
     2821 atttaaaagg atattcaaca ggttcacctt ctggtgctaa tgcaaacatc ccatatggtg
     2881 tgttcataaa tcctattaca gcaattgtaa gatttgatgg gtctaaattt gatttactac
     2941 ctagaaatgt aactgtattc actgatgaat taattacaac acatctaaca ccttcataca
     3001 ttacatgttt atcctttata tctccaaatt ttccaacaac ctttgccccg tcttgatgtg
     3061 gtaacatagt gtgcttccca ggtagtgctg gtttttgcgt ttgtgctact gctccaactg
     3121 ttggatatgc tgtcttatca aatggtaatg ggtggctaag actcttgtat atgatatcct
     3181 ttcctgctga tttattagca tcaatatcta catgatacaa acgcccatca ccaaaacgta
     3241 taagattcca ctggtggtca tttgatgtgc tgtgtacata ataagattca attcccatag
     3301 cgtcacacaa agctttcatg ctcctagcca ttcctgaaca tgcacctttt ttagtaacca
     3361 atgtataggc agtattcgct ccttctactg cattccagtc aacatatgtt gtgttatttt
     3421 taatgtgatt ataggcaact aaaatcttag cgtctgtagt catgttcttt gtaattttat
     3481 tgttcataaa atctgctgtt gcttgtgcta ctgagtttag ttgggcttgt gacagctttg
     3541 gaccatatac gctattggtt ccagtgacat agctttttgg tgtttttgct gttacctctt
     3601 tggctgtcat accataaact gtcatactca tacctgagac taaaacaatt gttgtaatca
     3661 ataacttggt agctaacttc ctaattttct tcatacttag tattctcctt tataaaatat
     3721 tgttacttcc tattctctac cattaataaa gttcatttgg gatttaaaaa ggtggattgt
     3781 caatatgtat gtttcttcat ttatacgttc gtgaaatatt taaatttcat ggaaaatcta
     3841 atagatattt tctagataat ttgtatacaa tacaactcgc aaatcagggt gtttgctgtt
     3901 agtttgagaa aatgatgatg attaatatat tcggaatctt tgacctataa taaataccat
     3961 tgtcttcaat cattaataat ggtaagagta aagagaatta tcaataatac agttaaaatc
     4021 aactttgtat acatttcata cattctctcc caacaacatt atacttctta taatttgtat
     4081 attcaatacg ttttctggga aaatatagga tatatctata aaaatataaa aaaggagttg
     4141 aacttaaaat ttgattaaaa aaaatcaatt ttaagacaac tcatttgtat tataaaaatg
     4201 ctatatatta ttattaagta agtcaatatt ttagatattt atctatttct tattgactta
     4261 cgcttatagt ctaacttatt tgtttagttg atatataacc tagttttaag cattattaat
     4321 atttaaataa tcaagtatcc tttttgtttc ttgtagtttc tgttctgcct catttaagtt
     4381 ttcttgaatt atagaaatat atattatatc tatcaacgta agcattgcta ttcttgaaga
     4441 aatagctcct tctctaagcc ttttttctat atttggtact actagcttta aatcactaat
     4501 cttactaaga tgattgtcac tatatttagt tatacaaatg accttacatc cttttaattt
     4561 tgcattttct atagacttta ttacttcttt tgattctcca gagtaagata ttgctatagc
     4621 tatatcttct ttatctacat ttgaagatac cataagttgt gtatgactat ctaaagaggt
     4681 aaatgcctgt ttatttatcc ttaaaagttt catctgtaaa tctagtgcaa ctaaagccga
     4741 accacctact ccaaatatat atacattttt agcctttatt atctgttcaa ctgcttcttt
     4801 tattatatct ttttcattca acaaccttgt ttggttgata gtctctatgt ttttgtttgt
     4861 aattttgttt ataacactat ctatattaca atcatagttt tcatctacat atgtgtattc
     4921 attttctttg cccatcaaat ctttagctat ctcaattttt aaatccccaa aacccgaata
     4981 tcccattttt ctagaaaacc taactatact tgctgtactt gtttttgagt ttgacgccaa
     5041 ttctgataca gaaagcttac ttatagcctc tgtattatta agtatgtaat cagctatttt
     5101 tttatctact tccgttaaat cttcaaatat ctgttttaat tttaaaatgc aactcatact
     5161 ctaccacctc tacctattta ctataatatt tcttcttagc aagtaaacat gctcctaaat
     5221 ctggagtcaa cactggtctc actataccaa atctcgtttt attcaaatat tttttaagtg
     5281 gctctaatat taaatctcct gacttaaaca ctccacctga ataagatacc tttatctttg
     5341 taccttgttc aaaatgatgc tctaatccta ttataagcct acttaattca taagctgcat
     5401 catcaaatat tttactagca gtattatcac cttcaataac tgcttttaaa catatcgtag
     5461 caaactttgc tatttctatt ctatctcctt gtattccatc atttacatat tttattatgt
     5521 cataatcatt ctctatatta agatgtcttt ttattaaatc atataatttt gtctttgaac
     5581 accttccatc agactgcatt gtgaacattt ttaaggtagc cattcctata taatacgctg
     5641 aaccttcatc tcctaataca tagccccatc ctccacatct gtgaagattc ccatctttat
     5701 ctaaagcctg tgctattgaa ccagtacctg caattatatt tatacctttt tccccattta
     5761 aagaacctgc taatgctatt tctacatcat tacctagagt ataatttata tgactatatg
     5821 cattttttgt tgctacttct aatgcataca atacttcttt tatttttcca tatccagcaa
     5881 gtccaacaaa tgcatacgtt atttcttcta catttatctt agcatcttta caaacttctt
     5941 ccaacccagt tattaataat ttcgttagat tatcaaatcc tatttgatta taatgacaag
     6001 ttcctttggt tatggttcct actatattta attcttcatc tactaatgta aatgttgttt
     6061 tcgttcctcc accatctaca cctaaaaaat acatactatc ctcctacaat atctttaata
     6121 attctttcaa tacttaatat ctaaaaataa ccttccaatg ctgttttttt aatattgaaa
     6181 ggttatcagt actattattt attttacaat ttttctattg attttaaagc ttcacttata
     6241 taaccttctt gtctatctag gatactcttt gcactatcct tatctaaacc actttttatc
     6301 ataaatatag ccaatttaac tctattatct gcttcttcta agaatttttc tgcaacgtca
     6361 tattttacat ttgtagctat cattattatt ctttttgctc tttcaaccaa cttttcattt
     6421 gatgtcttaa catctaccat caagtttgag tatacttttc ccatcttaac catagaagct
     6481 gttgttaaca tattacatac cattttttga gctgtaccac ttttcattct agttgaaccc
     6541 attattactt ctgcccctac tataatgttt attggtaaat ccgctttttt actcatttca
     6601 ctattcggat tcatagttat tgatagtact gcacacccca attccttagc atactccatt
     6661 gcacctataa cataaggagt ccttcctgat gcagctatac cacatataac atccttagat
     6721 gtaactcctc tatctattat atcttgcttt ccaagttctt tatcatcttc agcaccttct
     6781 attgctttaa atattgcttg atttccacca gctattatag cttgaacaag ctcaaaatcc
     6841 actccgtaag ttggaggaca ttctgtggca tctagtattc ctatcctccc actggtacct
     6901 gctcctatat agaataatct acctccctgc ttcattctat ctactattat attaacagct
     6961 tttgctatac tctccttttg ttcttcaaca gcataggcta cagttttatc ttcgttattt
     7021 attaattcta ttatgcctaa tgtatcttct ttatctattt gcaatgtatt cttatttcta
     7081 ccttctgtaa ctaaattttc taaagttttt attaacatat catttcctca ttttcttaat
     7141 attaaatttt taaacacatt ttaactccaa ctttcataat tatattttta taataaatta
     7201 atattttcat aataaattat tttgttgagg gtttaaaatc aaacttttgc catggtttaa
     7261 tataatctaa tataaataat tcttcttttc ttatatatcc tactacattc gatttaccac
     7321 tgtttttcat atcagtcaag gctatttgta actcccctgc ataatgacca tattcactac
     7381 tttcaattac tatatctcct ctttttatta ttttaggagt attaaatagt tcaaatttgt
     7441 atcctttata tttaacacga gattgagtag accttatcag actgtcagat atatcccctc
     7501 tattaaaatg tatttcttca aagagtatct tcttctcaac ttctcgtata ttttcctcta
     7561 ttgctacatc aaatgtaacc atatcttttc tcatagaacc taataccttt agctcctcat
     7621 atgttggata acaatttgat attattatat catttatatt attcatagct attatatgct
     7681 ttacttgaat atctataggt agatttctgt gcatctcaat tgttggaagc ccatcagtta
     7741 caggccatgg tccaaatgtg gtttcatttt gacttgttac aaaaactgct gttcttaatc
     7801 catacttatt aaatctttct gtacatttta taaagtgttc taaatttaat ccactatatc
     7861 tatgtggata aaagttatga catcctgtca aattttcttt atttggttgg tagtccatta
     7921 ttgtatctat ataattagta ttattactca tattgatttc tattttaaga ttttgaggat
     7981 taaaagtcat taaggccttt tcagaacctg taaatcccat atcaagcctt attccgtctg
     8041 ccttcgtctt tttaaaaaaa gtcaaatctt tgtagcttat ttctaattca ccaaatactc
     8101 tgggatttac atcaagtatt acctcaaaac ctttaaactt agcgtattca tttatacttt
     8161 taaatttttt aattatattt tctttagtat ctttaactga taataaacat gaaaatatac
     8221 gtgtaaatcc attttcagca gctttatcta tataatcata tatttcctta gatgttgttt
     8281 tttctggata aattgatatt cctaatcttc ccatctgtgt catctcctta tatcctatat
     8341 gtgtttaaat ttatttcgaa ttacacgaaa cttttttaaa aatttggtga aaaaaagttt
     8401 catatatatt tataaaaaaa gaaaagttta ccttttcttt ttttataaac aatcaacaaa
     8461 atatcttcgt tacttatttc ataactgtta tactttgaaa tttatactaa tctaatatag
     8521 tttttaattt ccaatatagt ttctaattaa taagcttctt gctcttcaac tttttcttca
     8581 tgaagcaatc tcttatcgta catcttgaca aatggatagt aaattattat tgatatagct
     8641 atacataata agttaagaac tgaagctctc caatctccac cagtggctaa atatgcccct
     8701 actggtcctg gtaatgtcca aggagcagta actactactc tggatactaa attagcactc
     8761 atagcaaacc atgaaactgt tgccattact attggtgcca caacaaatgg tattattaat
     8821 attgggttta atactattgg tgctccaaat attattggtt catttatatt gaatattgct
     8881 ggtattacag ttgcttttcc taaagacttt ccataagctg attttgctct aaatgcaaga
     8941 agtaaagcta agcctattgt agctccagaa cctccaatcc atataaacca ttgaaaaaag
     9001 ggttcagcag caatacctgg aactgcttga cccgcagcat atgaagctgt attagcatct
     9061 aataattgta tccataaagg tcttgccaat gaacctatta ttgatacacc atgtattcca
     9121 aatgaccaaa aaaacgttat taaaaatatt agtataatta ctgatggtaa tgtgtcagtg
     9181 gctgaaacta atggagttac taatttagct atgaagccat gccaatcaaa ccctaggaaa
     9241 taagtaacaa tagccattaa aattattatt aatgcggctg gtgtcagagc ttcaaaagac
     9301 cttgtgactg atgctggaac tgcttctggc atggttatct tgaatttaga tttgtcagta
     9361 aatctataaa tctcaacacc tattatagat gttacaatag caacaaacat tgaacctcca
     9421 ccaagattag ccattggcat ttgccatcct tgaggaattg aattatacca agctaaaagt
     9481 tctggactct tttcaactat ctttaataaa tcaggggtaa gaccctctat tgcagtagga
     9541 actattgtca aaaggaatgt agctgtagct aataaacctc ctgtcaatcc atctaactta
     9601 tatgactttg ccaaactata acctatgcca aatgtagaat atactgatat aataaaaaat
     9661 gtcattctat atggtagtaa tattgtaact gcattttcct ttagatactg agttaccccc
     9721 catgaatctg gtaatggagg aaatgctaat atcaagaaaa atgacccaac tattataagt
     9781 ggtaatgaag ctacgattcc atctcttaca gctcttaagt gacgttgttc tgccaatctg
     9841 gccatagaag ttgataattt actgtctaaa aattctgtaa atttatctag catcttttct
     9901 ctccttaatt aatatttatt tttattatca ttaaaatttt tataaaatta actttcttct
     9961 aacctaacaa cactgcaact actatttact caacaactct tgaacttgtt ttaataactt
    10021 tggtcctcct aatggactat aagcttgtgc ttgtattaat gcacatggta cactagcctc
    10081 atcagcaaaa gcttttaaat agtcaaatct atgtctgact tgtggagcta ccatagcaat
    10141 atcccatcca tttttaactt cattctcaaa ctcttgagta ccaacagcca aaacatctat
    10201 atctattcca ttttttgctc cttctttttg taatgcatta actgctatag cacttgacat
    10261 ccctgatgaa caaacaaata atactctcat attgattccc cctgtttttt tatttctttt
    10321 caatcattat agcatgtatt tttgattaca ggaatacgtt tgtatatatt ttttcatatt
    10381 ttacattttt aattgtaatt ttatttcatg catttatatc ttgctttaat tgctttattt
    10441 atctaatcat tcttttgtct aaaattacac actacttaaa atactcatat cttttcttaa
    10501 aaaagaaaaa agactatctt ttattaacat aaaatagtct caatactttt ttaaatatgt
    10561 attcctttat atatccagaa gtaacctatt attgcaaatg ataaaacaaa taaaatatac
    10621 agtctaaaaa atcccaaaga aaaacctctc ttagtttcaa caaagtatct tgtatatgca
    10681 actgcaaaag atgtaattaa tgaatttcct ataacaactc caaatctatt atctattcca
    10741 aatatagata ataacaaagg aaaaactaca gtacttacta gagatgaaac tattagtatt
    10801 aatccacttt taaaacttat agttggaata ttataatctg ttctttgatt tttctccatt
    10861 tctttcttag tatttttatt actttttgca tttttataat tattattttt ctttcttttc
    10921 ttttttttat tattagacat gtcatttacc tccaaaaggt ttaggtgtta tattccctaa
    10981 aataacttta tttttagccc ctgttgcaga tattacatta gaaaaagaat tatttaatgt
    11041 ttcattgccc aagattacaa atgccaaagc ctcctttgca tcagaagaat acccatattc
    11101 atcttgagtt aatacttcaa cctctaataa taactctttt atatatccta ttaaagttag
    11161 attatgaact cctccacctc caattaataa agtatctaca ttaacctttg gaagtatgaa
    11221 gtttttataa ttataatata tagcttttgc cgtaaacata gttactgttg ctatcaaatc
    11281 atatttatct acatgtttat atttgttaag catgttatcc aaatagactt gtccaaatac
    11341 ctctcttcca gtgcttttag gtggcgcttg actaagatac ttatgactca tcaaatcttt
    11401 taacatttct tcattaattt taccttttga tgcaaagtat ccatttttat catactttct
    11461 attaaataat ctttgacata ctccatctat tatcatattt cctggtcctg tatcaaatgc
    11521 aaatacatca tctatgttac atgatttagg tattatagta atatttccta ttccaccaat
    11581 attttgaagt gctaaatttt tatttttatc actatataat aaaaattcac tatatggtac
    11641 taatggagca ccttctccac ctgctgctat atccattact ctaaaattag atattacctt
    11701 tgtatttgtt tcatatgcta taattgctgg ttcacctatt tgaagcgttg aagatacaaa
    11761 attattataa cttcttggta tatgaaagat tgtttgacca tgagatgcga taaaatccaa
    11821 atttgctata tgaaagtttg ctttatgaca cactgatttt acagctttac taaataaata
    11881 tccaagctta aaatttaagc tacagattaa atcaacactc gattcctctt ctatacagca
    11941 ctttcttatt tcgtctttta tatctttagg aatttctaga gttataaatt ctatttgctt
    12001 aactttagta tttctaccat tcccactaat ttcagctagt actgcatcaa ctccatctaa
    12061 ggaagttcct gacattaaac ctacactata catagttatc atccttttaa gtttatagtt
    12121 attcctcccc aaatcaaggt attaagttta ataattttag aattaatctt atttcatctt
    12181 taattgcgat aatttactct tacgaaatta atcgagttaa tcttatacac gttttttacc
    12241 ttagctaaat cttacattaa tttattttca gtgtcaatgt aatgcatgta attttttttc
    12301 acaaacaaaa catattctct ctccaaaaac catattaaaa tagttactac aaataaaaaa
    12361 cataccaggt acctatctga tatgtttttc atatataata attattattt tataatatag
    12421 atagaacatc atttaaggca ctaattaaat cttcactatt ttcaagaact aaatttattc
    12481 ttattgtata tgtcgatttg tatatgatga tagattttat agtctaataa cctaactatc
    12541 taggcttaaa taatccgata tatcataaaa gtagatatta aaaattgtat atgaaccaat
    12601 tttcgaacat acctcaatct catgccctaa actatctatc attttcttta caaaatacat
    12661 ccccattcca gtagacttat acaattgatt tcttccattt ttccccgtaa atcccttgtc
    12721 aaaaacccta gatatatctt cctcatttat acctatacca aaatctttta tagataatac
    12781 cactccattt tctaactttt ttgaattaaa ttcaatctta ccaacttcct ttgaatactt
    12841 tatagcatta ttaataattt ggtctaaaac atatgacatg catttagaat ctgtgtaaac
    12901 atcattttct aattcattca aactaacttc tacattattt tttataagca aaaatgaatt
    12961 attttttata gacattttta ccaatttttc taaattaaat ttatttataa atacatcttc
    13021 aaacatagtg ttgcttctac tactatacaa aatagagttt actaaaaaat ttatctttgc
    13081 aacctgattc ttaatatcca aactatcttc tatgctactt aacctatctg taattatact
    13141 taaagaagat attggaattt ttatttcatg aacccattta gctatgtaat catctaattc
    13201 ttttaatgat tgtttataag tctctatgtc actattattt tttattttta agttttctat
    13261 aatatctttt attagatttt cttcaaaaga ttgtccatca attaaataac tgtctatttc
    13321 accactattt tctatacaat tatataaatt tatataacta tttctaaaat ttatataatc
    13381 tataacaaaa aataccatta ttataaacag cactaaaaaa tctaagtata ttaattctga
    13441 atatctattt tttaaaatat ctatagcaaa taaatagata tttattatac taactacaac
    13501 taaaggtaga gcaatatact tagacctacc ttttaaatat tttactagca ttatattata
    13561 tatcctatcc ccttcttagt ttttataaaa tcatcaaaac ctagttcttt aactttactt
    13621 ctcaatctat tcatatttac agtaagtgca ttgtctgtca caaactcatc attatcccat
    13681 agagtcatca taagactttc ccttgataca accctattct tattttttaa taataatgtt
    13741 aaaattttta tttcgttttt agtcaactct atacttttat ccttatactt atgttttata
    13801 atccctttcc ccacatcaaa catcaaatct tcacaataca aaatactatt agaatcacta
    13861 taattataga ctcttcttat tataccattt attttggtaa ccaatatgtc tatagaaaat
    13921 ggtttggtaa tataatcatc tgctccatta tttataccca ttattaaatc catatcagaa
    13981 tctcttgatg ataaaaatat taatggtaca tttgaaatag ctcttatttt ttcgcaccag
    14041 taaaatccat tatataatgg taaatttaca tctaaaagta ctacatctgg ttttttatcc
    14101 ataaattcat ctaatactac ctcaaaatca tcaattaatt ctactttaaa tccccatttt
    14161 tcaagtgcta acgcaatctc ttttgataaa tcaatatcgt cctctactaa taaaattcta
    14221 tacatttgta tattctccat tatcctaata tttcactatg ccatgaatta ttaatatcat
    14281 aacatatttt atataaa
//
LOCUS       DAEPOZ010000101        14192 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.101, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000101 DAEPOZ010000000
VERSION     DAEPOZ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14192
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.101"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            282..527
                     /locus_tag="KR440_002117"
     CDS             282..527
                     /locus_tag="KR440_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBH0699165.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            562..1287
                     /locus_tag="KR440_002118"
     CDS             562..1287
                     /locus_tag="KR440_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBH0699166.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEEENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            1309..2622
                     /locus_tag="KR440_002119"
     CDS             1309..2622
                     /locus_tag="KR440_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH0699167.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKVCGENLKKIV"
     gene            2921..3367
                     /locus_tag="KR440_002120"
     CDS             2921..3367
                     /locus_tag="KR440_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0699168.1"
                     /translation="MGGETHFLSFGKDEYKISLEEQENAIESAKASDNSVKLIAFIDD
                     EIVGIATIDSNQKAKGKHVGVLGIVVKEKYWGIGLGKRLMLDLIEWCKSNGITKKITL
                     VTNEENYNAIGLYKKVGFEVESILKKECYYNGVYTDLIGMSLLLGI"
     gene            3456..5441
                     /locus_tag="KR440_002121"
     CDS             3456..5441
                     /locus_tag="KR440_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HBH0699169.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTITLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIEEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGVKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            5665..6633
                     /gene="spoIIIAA"
                     /locus_tag="KR440_002122"
     CDS             5665..6633
                     /gene="spoIIIAA"
                     /locus_tag="KR440_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HBH0699170.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIDD
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            6623..7144
                     /locus_tag="KR440_002123"
     CDS             6623..7144
                     /locus_tag="KR440_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HBH0699171.1"
                     /translation="MQIKIIIIALLVGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            7161..7358
                     /gene="spoIIIAC"
                     /locus_tag="KR440_002124"
     CDS             7161..7358
                     /gene="spoIIIAC"
                     /locus_tag="KR440_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HBH0699172.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            7374..7760
                     /gene="spoIIIAD"
                     /locus_tag="KR440_002125"
     CDS             7374..7760
                     /gene="spoIIIAD"
                     /locus_tag="KR440_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HBH0699173.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            7845..9050
                     /gene="spoIIIAE"
                     /locus_tag="KR440_002126"
     CDS             7845..9050
                     /gene="spoIIIAE"
                     /locus_tag="KR440_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HBH0699174.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            9110..9727
                     /locus_tag="KR440_002127"
     CDS             9110..9727
                     /locus_tag="KR440_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HBH0699175.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            9739..10293
                     /locus_tag="KR440_002128"
     CDS             9739..10293
                     /locus_tag="KR440_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HBH0699176.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKKQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            10320..11009
                     /locus_tag="KR440_002129"
     CDS             10320..11009
                     /locus_tag="KR440_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HBH0699177.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDDSDSSKTEDSSNVDKQGGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            11119..11481
                     /locus_tag="KR440_002130"
     CDS             11119..11481
                     /locus_tag="KR440_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBH0699178.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            11566..12072
                     /gene="nusB"
                     /locus_tag="KR440_002131"
     CDS             11566..12072
                     /gene="nusB"
                     /locus_tag="KR440_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBH0699179.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            12056..13126
                     /locus_tag="KR440_002132"
     CDS             12056..13126
                     /locus_tag="KR440_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HBH0699180.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            13123..>14192
                     /locus_tag="KR440_002133"
     CDS             13123..>14192
                     /locus_tag="KR440_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBH0699181.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVMVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRG"
BASE COUNT         5773 a         1412 c         2590 g         4417 t
ORIGIN      
        1 tatagagatt aatttatact atgtcagaaa gaatattgta atttataatt aaatattaat
       61 aggtttttaa aataggatta gataggtaat aggttaagtt agaataaccc attacctatt
      121 tttatttgta agaaaagtgt acatagagtt aaaagtaaaa aatacaacta ataaaactat
      181 tgaaaacact agataaataa taaataaaaa taattataaa ttttggcata tatattgcta
      241 atcatagaga taagacgaca aaataattgg gaggtttaat gatgaaatac aaaacaacag
      301 ggacttgtgc tacagaaatt gaatttgaag taaaagaaaa taaagttaca aatgtaaact
      361 ttataggtgg atgtgatgga aatttaaaag ggctaaaagt attagttgaa ggcatgaata
      421 tagaagatgt aatacaaaag ttaaaaggaa tagagtgtaa aacaaagcca acatcttgcc
      481 cagaccaact gtctctagca ttggaaaatt atatgaatat aaagtaatac ataaaacaat
      541 cgattagtta ggagtttaat tatgaaacca ataataggga ttttaggaaa tttaataata
      601 atggaaaatg gtatgtttcc aggtcttgaa aggtcatatg taaataatga ttatataaat
      661 gcagtgctaa aaggtggcgg tagtccagta ataattcctg taaatacaga taaagaagtt
      721 ataaaaaaac agattgaaat ggttgatgga gtcttaatat ctgggggatg ggatatcaat
      781 cctcaactgt atggagaaga aacaagagaa gaaacgacat ttatatatcc agaagtggat
      841 gaatttgatt taattgcaat aagtatagca ttagaactta aaaagcctat attaggtgta
      901 tgtaggggac tacaaatttt aaatgtaagt ttaggaggaa ctttatatca agataataat
      961 cttatagaag gaagttatat aaaacataca caatcttcta aaagacatgt agctactcat
     1021 aaagtcgatg ttaaagaagg aagtatttta gagggaattt taggaaaaca attacttaca
     1081 aatagttatc atcatcaaag tgtcaatcag cttggaaaag gattaaaagc catagcatac
     1141 tcaaaagatg gtataataga agctattgag aaagaggagg aaaattttgt agtaggagta
     1201 cagtggcatc cagaaatgat ggtagattac tgtgataaaa tggaaaaact atttaaatat
     1261 tttataaata tttgttcaaa tttataatat accttggggg gattgaacat gaacgaaaag
     1321 aacaaaatgg gattaataag tattatactt ttaggtatta atgcagtagt aggagctgga
     1381 gtttttctat taccaggaga tgcaatgaaa tcatttggtg tagcaagtat atttgtatat
     1441 atatttgata tgttgttagt tttatctatg gcattctgtt ttgctgaggt tgcaggtaaa
     1501 ttcaataaaa atggcgctgc ctatgtatat accaaagaag cttttggaga tttttgtggt
     1561 tttgaagtag gtcttatgaa atgggtaata ggatgtattt cttggggagc tttaatagta
     1621 ggttttccta catcattatc agcagtatgg gctccagcag gtgaaccaca tatacaaaaa
     1681 attataattg tagccatgat agttgggctt acaattataa acttattagg tgtatctcta
     1741 tcaaaaattg tacaaaatgt tataactgta ggtaaactaa ttccattaat actatttata
     1801 ggaataggta tattttttat aaaaggagtt aattttactt cttcaacaat ggtacctcca
     1861 ggagctggag caacagagtt tggagcagca gcacttttaa tgttttattc atttacagga
     1921 tttgaatcta tagcagttgc agcagaagat atggaaaatc cacaaaaaaa tataccaata
     1981 gcaattataa gtgtaatagt aattgcatca ataatatata tattaaatca agttgtatgt
     2041 gttggtatac taggagattc gttatctagt acatctacac cagtggcaga tgctgcaaga
     2101 atatgctttg gaaatatggg agctggattg gttacctttg gaacattggt ttctgttggt
     2161 ggaatttgta tgtgtggtgc ttttgtaaat ccaagaagtt gtgtagcttt agctgatgat
     2221 aaaatgttac caagaatatt tgcaagaaaa gataaaaaag gtacaccata tgtagcgata
     2281 atagctacta tgttaatcac tataccaata gctctatctg gaagttttgc tgaattagca
     2341 gcaataagtg cagttgctag attcatacaa tacataccaa catctttatc cgtattagtg
     2401 tttagaaaga aaagaccaga attagtaggt acttttaaaa ctccttttgg agcagtgatt
     2461 cctctaattg ctgtatgtgt tggaacatgg ttgttattcc aagcatctat gcatcaatta
     2521 attatgggat taggagcttt ggcaattgga gtaccattat actttataat gaagtcttat
     2581 aatagaaaag tctgtggtga aaatttaaag aaaatagttt aattatgtta taagaattta
     2641 ttgagttaaa attagacatt ggcagaaaat taaatacaaa taagtatcta taaggctata
     2701 ttaaaatatt attaattttt tattttaata tagccttata ttgttatcaa taaatgaaaa
     2761 atattgtaac aacagaagaa caatagtatt atgtttatag ataaaaaact tattaggagg
     2821 taaatttatg ccaacaataa cattaaaaaa tggtgtagac gtacttatta gagaaggtgt
     2881 aagagaagat gcttaaagca ttatagattt ttataatgaa gtgggtggag aaactcattt
     2941 tctgtcattt ggaaaggatg aatataaaat atctttggag gaacaagaaa atgctataga
     3001 atcagcaaaa gcatctgata attctgttaa actgattgca tttatagatg atgaaatagt
     3061 tgggattgca acgattgatt ctaatcaaaa agcaaaagga aagcatgttg gagtattagg
     3121 aattgttgta aaagaaaaat attggggaat tggattgggt aaaaggctta tgttagacct
     3181 tatagaatgg tgtaaatcaa atggaataac caagaagata actcttgtca ctaatgaaga
     3241 aaattataat gctataggat tatacaaaaa agttggattt gaagtagaaa gcatactaaa
     3301 aaaagaatgt tattataatg gtgtttatac tgatttaata ggaatgtcac ttttattggg
     3361 aatataatta gtgtacattt tatctcgaaa aaagtatatt aagtatggta taattaaaaa
     3421 agaatataac ttaatataat tttggaggac gaattatgaa aaatctgtgt aaaatatcag
     3481 atgattattt aaacagcaag cttaagtatt taaagttatt atcaaaacaa tatccaagta
     3541 tatcaaaagc aagtacagaa ataataaatt tagaggctat attaaatctt ccaaagggaa
     3601 cagagcattt tataacagat gtacatggtg aatatgaacc atttgtacat gtattaaaaa
     3661 atggttctgg tgtaataaag agaaaaatag aagaattgtt ttcaaataca ataagagaca
     3721 gcgaaaaaaa aatgttagca actcttgtat attatcctga acaaaagtta gacctaataa
     3781 ttaagcaaga ggaaaatata gatgattttt atagaataaa tatttataga cttatagaac
     3841 tttgtaaata tgcttctagc aaatatacta ggtctaaagt tagaaaactg ttaccagaaa
     3901 attttaaata cataatagaa gaacttttgc atgagcatgt taaaagtgaa cataaagaag
     3961 agtattataa aagtatagtt gaaacaatag ttgacatagg aatagctaag gagtttataa
     4021 ttgctatttc tacagttata caaaaattgg ttgtagatag acttcatgta ataggagata
     4081 tttatgatag aggtccaaga ccagatataa ttgtcgataa gcttattgaa catcattgtg
     4141 tggatattca atggggaaat catgatatat tgtggatggg tgcagcctca ggtgaaaaaa
     4201 cttgtatagc aaatgcactg aggatatcag ctagatatgc gaatttagac attgtagaag
     4261 acatatatgg aataaatctg ttgcctttag ccacttttgc tatagaaatg tataaagatg
     4321 acccttgtaa agaatttatt ccaaaggtta atgaccaaag tgtaacaaca acagaaaagt
     4381 ctttaatggc aaaaatgcac aaagcaataa gtataattca atttaagctt gaaggtgagg
     4441 ttataagacg aagacctgaa tttgaaatgg aacatagact tttgcttaat atgataaact
     4501 atgatgaagg tacgattact ttaaaaggta aaacatataa attaaaagat acttatttac
     4561 ctacaataga caaaaaagac ccatataaat taactatgga agaaagaaat gttattgata
     4621 aattagtatc ttcatttaga ggtagtgaaa aattacaaaa acatgtttca tttttgtttt
     4681 ctaaaggaag tatatattta aaggctaact caaatttact gattcatgga tgtgtaccat
     4741 taaatgaaga tggaagcttt atgtctatga atataatggg aaaagagtat aaaggtaagg
     4801 ctcttatgga taagatggag tctctagcaa gagaaggatt tttctttaaa gataaggcag
     4861 aagaaaaact atatggtatg gatattatgt ggtatttatg gactgggaaa tgttcatcat
     4921 tatttggtaa agatgatatg actacatttg aaagatactt tatagaagaa aaagagactc
     4981 ataaagaaaa taagaatcca tattttaaac ttagagaaaa tgagatggct tgtaaaagat
     5041 tatttgaaga atttgattta gagcttgatg aatcccatat aataaatggt catgttcctg
     5101 tggagagtaa aaatggagag agcccaataa aagctaatgg taaaattctc gttatagatg
     5161 gtggtttttc tagggcttac caaaaaacta ctggtatagc tggatacact ttgatataca
     5221 attctagaac tttgcaatta gtatcgcatg aaccatttaa ttctgctgaa gaagccattg
     5281 caaatgaaag tgatatctta tcgacaactg ttgtagtaga gcataaggca aagagaaaaa
     5341 tggtgagaga tactgatgaa ggagtaaaaa tacaagagga aatagaagat ttaaaactac
     5401 ttttaatggc ttataagaaa gggctaataa aagaaatgta gatattatag aggcacttca
     5461 taattgaagt gtcttcttgt taatacttat taaatatatt ataaagatta ataatagtag
     5521 tttatattat acttactaag tgaatccaag ataaatgggt tcactttttt tatttataaa
     5581 cattattaaa aaaatataga tgttttggtt atattccttg tcctttataa atagaattat
     5641 ataaacaaag gaggaggaca agagatgaat aaactttctg atgaaataat aaactctctt
     5701 tctacaacta taagagaaaa ggtagaaaaa gtctcaaaca ataatctgaa catagaagaa
     5761 attaggttac gctcacaaaa accattgata ctaaatgcaa actctaaaga ttatttttac
     5821 aatcaaaaaa ctatgacact tgatttaaat caacaaaact catatgtagt aacaagagaa
     5881 gatgtagagc aaacttttca aataatatgt aaatattcaa tacattcatt tatggatgac
     5941 ataaaaaaag gattcataac tttgagagga ggtcatagag tagggttagt tggaaaagca
     6001 atagtagaag atggacaagt caaaaatata aagcatatat catctttaaa tataagggta
     6061 tcaagggaaa ttattggatg ctcggataag attttaagtc atattataaa gggaaaaaat
     6121 cagattaata atactttgat aatatcacca cctcaatgtg gaaaaacaac tttaataaga
     6181 gatatagtaa gaaatttaag taatggtaat gaagattatg gatttaaagg tttaaaagta
     6241 gctttggtag atgagcgtaa tgagatagca ggagcttatc taggtgttcc acaaatggat
     6301 gtgggaataa gaactgatat aatagaaact tgcccaaaag atttgggtat tacgatgctt
     6361 ttaaggtcta tgtcaccaaa tgttattgtg acagatgaaa ttgggagtga aaaagaaata
     6421 aaggcattat acacagcatt aaatggaggt ataggattaa taactactgt gcatggagat
     6481 tcaatagatg atatacaaaa tagaaaagaa ttaaatagac ttctggataa agagttattt
     6541 aaaaaggtta taattctttc tgcaaaaaga ggagcaggaa cgattgaaaa aatttatgat
     6601 ttagaagaaa agagatggta ttttgcaaat taaaataatt attatagctc tcttggtagg
     6661 aagtagttat ttgataggtg aacaaatata caaaacttat actcgtagac ataaacaatt
     6721 aaacgattta ataagagttt tagaaatttt gaggatggat ttatctttcg gactttatac
     6781 attagaagaa atctttaata ggataggtgg aaataaagag ttttgttttt ggaaattttt
     6841 ctatcaaata tcagaaggac ttcacaatga acaaagtaaa actttggaaa taataatatc
     6901 agaaaatatt gatgtcttaa gtaaagaaac ttatctaggc aataaagaaa ttgaggaact
     6961 gaaaaatcta atacttactt tagggaaaag cgatatagag tcacaacaaa gaatgataga
     7021 tttatctata gaaaatttga aaaaacaaac ctatgagaca aaggaagata taaacaaaaa
     7081 aggcgtattg tataaaaaat tagtgacatt cataggtata ggtatatgta ttatattgat
     7141 ttaatatggg gaggtttatt atggaaatat cattgatatt aaaggttgca ggtgtcggta
     7201 ttctaatatc agtattgaat atgatattgg aaaaaacaga tagaaaagat tgggcaggtc
     7261 ttactacatt agcaggtgta ataatcgtat tgggtatggt aataacagaa ataagtgact
     7321 tgtttaatac tgtaagaaca atgtttcaac tttactaaaa aggagggagt aatttggaaa
     7381 taatgcaatt aataggaatt gcaatcattt caactacact ctgtttagta attaaaaaag
     7441 atagaccaga gatagcaaat ttcatagcaa taataactgg agtaataata ctattgtcag
     7501 ttatgtttaa gctaaatttt atagtagata gtatacaaga tttggctaac aaggctaaca
     7561 taccgactat gtatatatca ttaataataa aattaattgg gatagcatac ttaatggaat
     7621 ttgcgataca actttgtaaa gattgtgggg aaggaaacat tgcttcaaaa ctagaatttg
     7681 gaggtaaaat aatagttatg tcaatgtctt tccctatact tttgtccatt gtagaaatgg
     7741 tggtgaatat aattccatag atgtgtatct tggaatgaat ataagtatat tagagaactt
     7801 tcgttgtatt gaggtttata gagtgaagag aggcatggtt gcttatgaaa aaaagatttt
     7861 taccaatgat tataggtttt ttatttactt tcttttttgt aaatatcttt gctattatga
     7921 tatttgctaa tgaagtacca agtagtgaag ataaagaata tggtgaaaca aaaaatagca
     7981 tagataaata tatagatggt caattagaca agctagatat aaatgagatt caagactata
     8041 taaataaaga aatagttatt aatgatgtta atttaaaatc atttgtaaaa gatttaatta
     8101 gtggagaaaa aaatatctta gatttattca ataaagatgg attgaagata ttgatgtttg
     8161 atgaatttaa agcaagttta aaggtagtag cagtaatttt ggtactggct ctgttatcat
     8221 ctattttaaa gagtctggaa aactcttttt catcaggtgc agtaagtcaa atagcaacat
     8281 atataatatt tatcacaatg gtatcactta cacttattgg ttttaaagat gtattacaga
     8341 tttgttatga tgcaatagac catactgtag gtcttatgca agttataatg ccaatattaa
     8401 taacattttt acttttaatt gggttcccaa taacgtcgac tactttgaat cctatcttca
     8461 taggaggagt aacatttata aatgtatttt ttaaaaattt tctatttgta tctattactg
     8521 ttgcatttgg aattttaata ataaacaatt tatcaaaaaa tataaggcta aagagatttt
     8581 tctcctttgt aaaacaaata aattatgttt ctattggagc tatgtttact gtttacttag
     8641 gtcttgtatc catacaagga ttgtatgtaa ccagttttga taaattcagt gtaaagactg
     8701 ctaaatttgc tataggtaat tttattcctg ttgtaggtgg atttgtatct gactctgtgg
     8761 atattttatt atcttcatct cagcttataa aaaatatatt tggaggaata gggcttatct
     8821 tacttgttgg aatttgttta cttccagtaa taaaaatttt atcagtaata gtagtgtaca
     8881 aattagcagc aataattgta gagcctgttg gagaggatgg aatatctaat tttttaaatg
     8941 aagtggcaaa tttaatgata ataatgctag catcagtaat tgctataaca gtaatgttct
     9001 ttgtaactgt agctatcttg acctcgataa gtgtcgtaag tcaagggtaa tttatatatc
     9061 agtgaaaaat aattacattt aatctaatgt ttttagagag gaggatgcta tgttagaggg
     9121 cataaaggca tggatagtta gtgttttgat aggagcattt atagtaaata tagtagatat
     9181 gattttacca agttctaaga taaaacctta tgtcaaccta gttttaaact ttatgtttgt
     9241 atttattgta ataactccag tagtaggatt tttttcaaaa gatatgagtt tagaagatag
     9301 aatattaaaa tctatgggaa actacaacaa gcagtatgta gatagtacaa atgctttggc
     9361 aaaagagact ggaaataata gtctatccaa gggatatgag gatgggttaa aagaagtact
     9421 taaattaaaa cttgatgaat atggttatga tttagaagat atagagctta atggggcaaa
     9481 tataaataat ataaaaataa aagaaaagaa taatagtgca aaaagtaata acagttccaa
     9541 tgaagaaaat aaaaataata gtactaaaag agaggaagaa aacataaact ctaatgacaa
     9601 agaaaattct aaacaagtct tcaaaaaagg tacagagtat gggttgaacc taaatgaaga
     9661 aaaattaaaa aacgacttaa taaaggtgct ggatgtttct atagaagata tacaaattga
     9721 taaataggag ttgggagaat gttgaaaaac ttaaatgaca aagataagag aaaggtatat
     9781 tcactaataa ctatagcagg aatatgtgtg gtttcattag tattattatc ttgttttcct
     9841 agtagcaaaa gtgataaaca agttggaaaa acagagacta ataaaaatgc tgaaaagcaa
     9901 gtcactaaaa aacaagaaaa agatgattta gagtcaaaat taacagcaat actatctaaa
     9961 attgatggtg caggagacgt tgatgtaatg gttacatttg aatcaagtga agaaatacaa
    10021 cctgctttta attcaaataa tacaacagaa acaacagagg aaaaagatgc acaaggtgga
    10081 gaaagaacag ttacgacttc tagtgaaaat aaaacaatga taacttcaaa ttcaagtgac
    10141 cctgtagtta taaaaactac agagccaaag ataaagggag tgatagtggt agcaagtgga
    10201 gcaagtgacc caagtgttaa agagacactt tatagtgcag tacaaacatc attacaagta
    10261 gctggtcatc aagtagaaat atattctaaa taaatacata taaccggggg gataaatgaa
    10321 tgaagtttaa ttataaggga agaggatttg taataataac tttaactgca atgttagtgg
    10381 tagtaggaac agtgaattat caactaagta agaaatcttt attagaaaca tcaaaagagt
    10441 ttaaggcata tgaagaagca cagttgcaaa aaaatactga tgatagtgat agtagtaaga
    10501 cagaggattc tagtaatgta gataaacaag gtggtaagga aagtgctgat attgatatag
    10561 tcgatagcaa agctagtaaa gtaaaagaaa aggcaactga gacaagtaag gaaataaaag
    10621 ctcaattatc atcagagaaa aatatgaaaa aggcatctta tattttagat atgaaaatga
    10681 atagagaaaa acaaagaaat gagttagttc aagatttaaa tgaaatgata aataatccat
    10741 ctacaacaga agaatctaga aaagaagctt ctaatatgaa attaaatata gttaaaattc
    10801 aagaaaaaga acttcaaata gaaaaccttt tgagtacaaa aggatatgaa gaagcgttag
    10861 tttatataag cgataacaaa gttaatgtag ttgtaaatga agctaaatta gaaaagaaag
    10921 atgcagcaaa aatatttgat ttagttgcag aacaagccaa tgtgaagtat gaaaatatta
    10981 aacttacaaa taataatagt aataagtaaa gtataaacct cagaattaac tgaggttatt
    11041 ttcttgcaaa gtagacttgt atttggtatt attatactta taaaaatagt tccttaaaat
    11101 caggaggtaa gttttaatat ggaagataat aaatttggac aagtaaaaat atctaatgat
    11161 gtaatagcta caatagctgg tttagcagca ttagaagttg aaggcataga aactacagca
    11221 acattaacag ataagttgtt aaaaaataat ggagtaaaaa tacaaatcga agaagaggat
    11281 gttaatttag atgtaatggt tacgataaag tatggaatgt caataccaga tacagctttt
    11341 aaagttcaag aaaatgtaaa aaatactgtt gagacaatga caggactgaa ggtttctcaa
    11401 gtaaatatac acattcaagg aatcagcttt aagaaggata aagttgataa agaagaagca
    11461 aaagcaacta agaaaaatta atattagccc tctgaaaagt cagagggttt tttgctggta
    11521 attgatttta tatggataat taaacttaaa ggagaatttt gattgatgaa aaaagatagg
    11581 gcacaaaaga gtacaacaag agaatatata atgaaactta tataccaaat aaatattaat
    11641 aaagaagatt ttgaaacttt agaagataaa gtagataatt ttttaaaaga taattcagaa
    11701 catattataa atagatataa agaacttgca cttcaatact caaaaaatac aaatttaaag
    11761 ttagaagata ctgaaattga agatgttata gacaagaaat atataaatac agtatgcaaa
    11821 gcattaaaag aaaatcatga caaaatagat gagttaataa ataagcatgc taaaaactgg
    11881 acagtagata gaatgccaaa ggtagatgtt tctatactta gattgtctgt gtgtgaaata
    11941 ctttatttag acactcctaa taaagtatca ataaatgaag cggtagaact tgcaaaaata
    12001 tattgtgatg ataagtctcc taaatttata aatggtatat tgggaagtgt tgttgatgaa
    12061 attgggaaat aatataataa ttggaattga tactagctgc tatactactt ctatagcagc
    12121 tatctcttta gataagaaag ttatattcaa tgaaaaaata atgttagaag taagggataa
    12181 ttcaaaaggt ttaaggcaaa gtgaagctgt atttcaacat atcaataatt tagggattct
    12241 tagtgatagg ataaaatctt ttaaagataa gtttaatgta gaaggagttt gctcatcaaa
    12301 aaaaccgaga cctgttgaaa actcatatat gcctgtattc aatgtaggtc ataattttgg
    12361 aaaattattg tctagtatat atggttgtag gttttatgag actactcatc aagagaatca
    12421 tattgaagca agtttattaa atagtaaatt aaaaaataat aataaattta tttctgttca
    12481 tatgtctggt ggtactacag agatactttt aacaagcaaa caagatagtc atcacaatgt
    12541 atgtgatact aatttaggta aaattgccaa aataagtatt aaaaaagatg ataaaagtaa
    12601 gttatacaat aattttggat ataatataga tataattgga ggaagtaaag atattagctt
    12661 tggtcaactc atagatagag taggaattaa gcttggatat aaatttcctt caggaaagta
    12721 tttggatgaa aatgctttaa attgtaatct taaaatagaa agtggtctga aaacttctgt
    12781 aagagatggg tatatgaatt tatctggatt agaaaatcag gtaaataaga ttataaatga
    12841 taatggagat aatactaatc aaaaagaata catatctaaa ttagtattag attcagtagt
    12901 tagaaatatg tttaaatctt tagtatattt atgtgaaact tataatgtaa atgaagtagt
    12961 atttgctggt ggagtttctg caagtaagta tattcttaga gaattaagca tgaagttaag
    13021 aaaaaagcat atagaagctt attttacaga accacaatat tcaactgata atgctgtcgg
    13081 gtgtgctata ataggattga ataacttttt aggagaaaga gtatgaaact aagagcttta
    13141 gatataagtg aagcaaactc atatattaaa agaattttaa taaacgaccc aatactttct
    13201 aatcttaaag ttaaaggaga aatttctaat tttaaggttc atagtagtgg gaatgtatat
    13261 ttatcactta aagatgaaac atcaaagtta aactgtgtaa tttttaaaag taattttaat
    13321 cgcaatttaa aattagataa tggtgtaaaa attatagcta atggatatat atcagtctat
    13381 gaaagagatg gtgcgtatca attatacatt aatgaaattg aaatagaggg aataggaaat
    13441 ttacacatag agtttaatag attaaaggaa aaacttaata aagaaggatt atttgaccct
    13501 aaatacaaga tacctatacc taaaatgcca aactcaattg gcgttataac atctccaaca
    13561 ggtgctgtta taagggatat aataaatgta ataaaaagaa gatatccgaa agttaatata
    13621 aagctttatc cagtaatggt tcaaggagat aaatcagctg aagaaatctg tgaagcaatt
    13681 agatttttca atcatatgaa gaatgttgat acacttatag ttggtagagg tgggggttca
    13741 atagaagaac tttggtcttt taatgatgaa atggtagcaa gagaggtttt taattctcaa
    13801 attccgataa tatcagcagt tgggcatgaa acggatttta ctatttgtga ctttgtgtct
    13861 gatatgagag ccccaacgcc atcagcagca gctgagattg ctactccatc attggatgat
    13921 ataaactata aacttggaaa cataaaaagt aggatgagta agtctcttac aaatcaaata
    13981 gagctagacc aatataggtt agaaacagta tttaataaga taaataacta tttagattcg
    14041 tacacaataa aagataaagt tatacaatta gacaaaatat atgataaaat aatttttgga
    14101 atagaaaata atttaaaatt agaagatgaa aaattagtaa aaattggagc attactccat
    14161 aatttaagtc cattagctac tatggataga gg
//
LOCUS       DAEPOZ010000102        13489 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.102, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000102 DAEPOZ010000000
VERSION     DAEPOZ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13489
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.102"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            285..1469
                     /locus_tag="KR440_002134"
     CDS             285..1469
                     /locus_tag="KR440_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699182.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
     gene            complement(1846..2955)
                     /locus_tag="KR440_002135"
     CDS             complement(1846..2955)
                     /locus_tag="KR440_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBH0699183.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            3418..4611
                     /gene="nifV"
                     /locus_tag="KR440_002136"
     CDS             3418..4611
                     /gene="nifV"
                     /locus_tag="KR440_002136"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HBH0699184.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVI
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            4636..6561
                     /locus_tag="KR440_002137"
     CDS             4636..6561
                     /locus_tag="KR440_002137"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HBH0699185.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWIELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSNVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            6583..7578
                     /locus_tag="KR440_002138"
     CDS             6583..7578
                     /locus_tag="KR440_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HBH0699186.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGAEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            7905..8378
                     /locus_tag="KR440_002139"
     CDS             7905..8378
                     /locus_tag="KR440_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0699187.1"
                     /translation="MVGSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDEFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            8412..8906
                     /locus_tag="KR440_002140"
     CDS             8412..8906
                     /locus_tag="KR440_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0699188.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            9003..9524
                     /locus_tag="KR440_002141"
     CDS             9003..9524
                     /locus_tag="KR440_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH0699189.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMYDDNIEADKYIREELKVPSKYSVECIISIGYSDEEKKAYND
                     SDLDYKKVHFNNF"
     gene            9713..10024
                     /locus_tag="KR440_002142"
     CDS             9713..10024
                     /locus_tag="KR440_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBH0699190.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            10052..10738
                     /locus_tag="KR440_002143"
     CDS             10052..10738
                     /locus_tag="KR440_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBH0699191.1"
                     /translation="MKYSYKKCIIDSILLMFIIQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
     gene            10970..11854
                     /locus_tag="KR440_002144"
     CDS             10970..11854
                     /locus_tag="KR440_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBH0699192.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQNSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
     gene            11953..13128
                     /locus_tag="KR440_002145"
     CDS             11953..13128
                     /locus_tag="KR440_002145"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HBH0699193.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEELLKDIPTANKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            13256..>13489
                     /locus_tag="KR440_002146"
     CDS             13256..>13489
                     /locus_tag="KR440_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699194.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSNESDARWYVQWWNRYLYKNINKEESLKSIIN"
BASE COUNT         5216 a         1454 c         2432 g         4387 t
ORIGIN      
        1 ttattataaa attataaaga aaaaattaaa taataataaa gaaaaaatgt ataatattta
       61 acattaaaaa agatatttca taaaattaaa aaacaaagtg aatttgaata ctatttaaaa
      121 aataaaaaaa tatataaaaa atatttaata caaaggtaga aataagttga aaatgtaaag
      181 tcattggaaa aatatagttg aatttaatga acaaaaatgt tattgtttta tcaatgaagt
      241 gttaaaataa aaactttcaa tatcatttta gggaggatga tttcatgcaa aaaagtgtaa
      301 agaaagcaaa agtaacggga tctatgttag ccatttttgg tgttgcaagc gttcttttta
      361 gttcacatgc aggaggagga tttgctacag ggaatcaaga aactcagtac tatgtgcagt
      421 atggatggac agcaccactt atggcaattt tagcaatgat tatacttact gctaccatga
      481 gagaagttat cataatgtat aataataaca attgtaggaa ttataaggat ttattttgtg
      541 agctttggag accatatcca aaacttgaaa taatatggga gatatattac tatctaatgg
      601 ttttaatagc tgtaagtgct gttatagcag gtgcagcagc agtatttcaa agtataggag
      661 taaattattt tgtagctgta tttattattg gagttgtact tcttgtcttt actatatttg
      721 gtgctatgct tgtatctaaa gcagctacag caatgacaat agctattcta gtatgtactc
      781 ttactatttt tattgttggt ataaaagcta aagtaccaga aataactgaa atattatcaa
      841 atagaacgag tttcacacct ggctatatca agccaatact taatactttt atttatgcag
      901 gttttcaatc ggttgttata cctacacttg caggatgttc aagaccactt cttaagaact
      961 caaaagaagc tactaaggca atgataattt catttgttat gaatgcaatt gcattaggac
     1021 ttgctgtaac tatgctaatg ggatggtata gagaaattat tgctgctggg caaacaacac
     1081 tccctactct ttatgttgca ggtcaatctg gaaatcatac tatatacata atttataatg
     1141 ttgcattatt cctttgttta atgtctacag gagtaactac tatatttggt cttgtaaata
     1201 gatttgaaga ccataaagca ctttctttcc tttcttcaag aatgaaaaga agggtattta
     1261 cagcttgtgc aataatggta gtgtcaatgc ttatatcact tacaggatta agtaatattg
     1321 ttaaatatgg atatggatat tgtggttatc ttggtctatt cactatagta attccattcc
     1381 ttacactagg acattataag aataagaagt ttgcaaagga aaatccagaa gctaaatggc
     1441 cagcggaatt aaatgaaaat gtaaattaga tgtagtagca aaaaatattg ctaaagagaa
     1501 tactataaat aagaatacta tagataatat atctgcatgt ttattaagat tatttgcaaa
     1561 atctaatatt gaacaactat aaacaagcaa tataaaacta agaataaaag ataaaaagag
     1621 ggtgtctcaa aatggacttt tagtttataa ggcactcttt tttatatgaa gtcaaatata
     1681 ttttcattat tttgacaata aaagagacta atcctacttt ggatagcctc ttttttcttt
     1741 aatataaaat agtataacag caaagtatag attgatacat gttttctgta gctttctata
     1801 tattacagtg tttgtgtggc attggtattt taagcaatct ataaattata attctataat
     1861 tcttctacta attttagatt ctatagccgt ctctatttca cttaacattc gttcattttt
     1921 agggtcaata aaagcacatg tatagccttc ttcaccagct ctaccagttc taccaatacg
     1981 gtggatgtag tcttctggtg tttctggtaa atcgtaatta tatatatgac ttactccaga
     2041 tatatcaagg ccacgagaag ctacatctgt agctattaag tattgtatat ctaaatttct
     2101 aaatgatttc attattcttt ctcgttttga ttgagcaata tcactatgaa gtttttgaca
     2161 attatatcct cttatagcaa gagcttcttc aagattgtca acccttctct tagtcctaca
     2221 aaatattatt gccataaaag gattatcttc atctagtacc ttacataagg catctaattt
     2281 ttttctatct gtagtttcta ctacttcttg ttttatatta ttaagagtga tttcttcttt
     2341 ttgaatagaa actacaacag gattttttgt gtatctataa gctaattttt ttacttgaga
     2401 atttatagtt gcagaaaagc atagtgtttg ctttttgttt gaagtctctt ttaaaatagc
     2461 ttccacttcg tttttaaatc ccataagcaa catttggtct gcttcatcta aaacaaaagt
     2521 ctttaactta ctaaggtcta ttgtttttct atttagatgg tctaaaagcc tacctggtgt
     2581 agctataatc atatgaatat ttccttttag tttctttatt tgagaaccaa tatcttttcc
     2641 tccatatgct gcaagaatac ttacatcttt agattcttta agtttcatag cttcttctgt
     2701 tatttgaata gctagttctc ttgtaggaga aagtataagt acttgtatat catttatatc
     2761 taatgatata ttttcaaata tcggaagtaa aaaagctagt gtttttcctg ttccagtttg
     2821 agcttctgct attacatctt ttccttcttt tattagttta atactttctt tttgtatagg
     2881 tgtaggtgtt gttattcctg atttttttag aatatttatt gtattttcac taatccctaa
     2941 gcttttgaaa tccattgtta tcttaccttt catgaaaaat tttctttttt attaaccaat
     3001 taataatatc atatttgtaa gtatttttag taaatttttt aattattatt tatatagctt
     3061 aaaaatatat taagatactt taaaatggaa tgtaacttaa cttaaatttt agtaggaatt
     3121 ttataggttt atttttaaag gttttttgaa aataaatcta tttaaaaatt aaattaaaat
     3181 gcaagaaaaa cataataact ttcattttta agaagttttc acaaaaaaga aaaaatatag
     3241 tgcaataaat gatacaaagt aatataatag attacaagat atgaaaagct aaaaaaataa
     3301 attgcaaatg aaattaaaat gaataaaaaa taaaaaaata atcataagtt agaggtatca
     3361 atgaaaaata agacttaatg tttttaaaat taaaagtata ctaagggggg cattaatatg
     3421 tgcgttatat caaaagatag agcaaaggaa ataaaaatag tagatactac tttaagagat
     3481 ggagagcaaa cagctggggt tgtatttgca aatagagaaa aaataatgat tgctgaaatg
     3541 ttaagtgacc taggtgttga ccaaattgaa gttggtatac ctactatggg tggagatgaa
     3601 aaaaatgtaa taaagcacat atgtagtaga aatttaaaat cagatataat ggcttggaat
     3661 agagcagtaa taaaagatgt tgaagaatct atttcttgtg gtgttgatgc agtagctata
     3721 tcaatatcag tatctgacat tcatatagaa aataaattga gaacttctag aggctgggtt
     3781 ttagaaaata tggctaaaac agttgaattt gcaaagaaaa atggattata tgtttcagta
     3841 aatggagaag atgcttcaag agcagatata gacttcttaa ctgaatttat aaatgttgga
     3901 aaacaagcag gggcagatag atttagatac tgtgatacag taggagttat gaatcctttt
     3961 tctataaaga atgcaataga aactctttat gaaagaacta attttgatat agaaatgcat
     4021 acacataatg actttgggat ggcaactgca aatgcacttg caggaatagc agcaggagca
     4081 aattatgttg gtgttacagt aaatggatta ggtgaaagag caggaaatgc agcattagaa
     4141 gaagttttaa tggcacttaa gtgtgtttat aaatgtgatt taaataatat agatactaga
     4201 aaatttagag gaatatgtga gtatgtagca caggcatcag gaagaatact tcctacatgg
     4261 aaaccagtag ttggggataa tatgtttata catgaatctg gtatacatgc agatggagct
     4321 ttaaaagacc cgcataacta tgaaccattt gacccaagtg aagtaaatct tgaaagaaaa
     4381 atagtaatag gtaaacattc aggaagagct gcagttataa ataagttgag tgaatatgaa
     4441 atgtatattt ctccagaaaa cgctaccaag ctattaaatg ctataagagc tacatctata
     4501 agattaaaaa gaagtttaat ggataaagaa attcttcaat tatattgtga tatactagca
     4561 catgagaaag gcacaacaga agaagaagca gttagaggtt catatattta agatataatt
     4621 gtttaggagg aaaatatggg agataatata gtttataaaa taattaaaaa acatatagta
     4681 gatggagaag ctgttgctgg aagttcaatt ggtataaaaa tagaccaaac tttaacacaa
     4741 gattctactg gaacaatgac atatcttcaa ctagaagcta tgggaataga taaagtaaag
     4801 actaaaagaa gtgtggcatt tgtagaccat aatatgttac aacaaggatt tgaaaatgca
     4861 gacgaccata agtatataca aacagtggca gataaatatg gagtttattt ctctaagcca
     4921 ggaaatggta tttgtcatca agtattttta gaaagatttt caacaccagg agatacactt
     4981 ttaggttctg atagccacac acctactgct ggaggtgtgg gcatgatggc tattggagct
     5041 ggtggattag atgttgcttt agccatggca ggaggagcat actatataaa agcaccaaaa
     5101 gtctgtaagg tgaatctagt tggaaaatta aataatatgg tatcatcaaa agatataata
     5161 ttagaagtgt taagaaaaca aactgtaaaa ggtggagtag gtaaagttta tgaatatggt
     5221 ggagaaggtg tcaaaagcct atctgttcca caaagagcta caataacaaa tatgggggct
     5281 gaacttggag caactacttc aatcttccca agtgatgaaa aaacactaga attttttaag
     5341 agtcaaggaa gagaagacgc atggatagag ttaaaaccag atgcagatgc agtttatgat
     5401 gaagagatta ctataaactt ggatgaatta aaaccattag ctgcaaaacc tcatagccca
     5461 gacaatgtag atgaagttga aaatataggc aagattaaaa tagaccaagt ggcaatagga
     5521 agttgtacaa attcttctta tgaagactta atgaaagtag cacaaatcct gaaaggaaat
     5581 aaagtacata aggatgtgag tttggtaata gcaccaggtt caagacaagt tatggagatg
     5641 attgctagaa atggggcttt ggcagatata ataagtgctg gagctagaat actagaaaat
     5701 tcttgtggtc catgtattgg tatgggtcaa tctccaggta ctgactctgt gtctttaaga
     5761 actttcaata gaaatttcta tggtagaagt gggactttgt cagcacaagt atatttagta
     5821 agtccagaag tggcagcagt aagtgcaatc aagggagtgc ttacagaccc aagagaattt
     5881 gatattaaat ttacaaattt agatgtaaat gaatttttga tagatgatag catgataatt
     5941 aaacctgctg atgtaggaag taatgtagaa gtagttagag gacctaatat aaaacctttc
     6001 cctcttaata cagagctaag tcagtctata ggtggaaaag ttatattaaa gacagaagat
     6061 aatataacta ctgaccatat aatgccatca aatgctaagt tacttccttt tagaagtaat
     6121 ataccgtatc ttgcaaacta ttgtttcaat actgtagata cagaatttcc tcagagagca
     6181 aaagacaata atggtggatt tatagttggt ggagataatt atggtcaagg ttcaagtaga
     6241 gaacatgcag ctttagcacc attatatcta ggtgttaaag gggtaatagt taagagcttt
     6301 gcaagaatcc acaaggctaa cttgataaat agtggtataa ttccaatgga gttttgtgat
     6361 gagaaagatt atgaaaatat atctctttta gataatctgg agataccaaa tattttagac
     6421 aatctaggta gtggaatttt agaagttaag aatacaacta aaggaacttc ttttaaggtc
     6481 aaagtagaat tgtcagcaaa agaagttgac gtattaaaag ctggtggaaa attaaactac
     6541 actaaaaatc aagctaatta atatgaaaag agggaatatt gaatgtataa agtgacactt
     6601 ataccaggag atggaatagg accagaagtt gcgaaagcta tgaaaaaggt agtagaagct
     6661 actggagcag aaatagaatg ggaagaagtt aatgcaggag aggcagttat tgaagagtat
     6721 ggtactccac ttcctgaata tattatagat agtataaaaa agaacaaaat agctataaaa
     6781 ggcccaatta ctactcctgt aggtaaagga tttaggagtg taaatgtaac tttaagacag
     6841 gcattagatt tgtatgtcaa tttaagacct attaaaagtt ttaaggggat aaaatcaaga
     6901 tatgaagatg ttgatttagt ggttgtaaga gaaaatacag aagatttata tgctggaata
     6961 gaacataaaa taggtgatta tgcagctgaa agtataaaga taataacaag aagtgcaagt
     7021 gaaagaatcg ttgattttgc atgtaactat gttaaagata ataagagaaa aaaagttaca
     7081 gctatacata aggcaaatat aatgaaaatg tctgatggat tgtttttaga tgtgtttaga
     7141 gaagttgcat caaaacatgg tgtagaatat gatgatttga ttgtagatgc agcagctatg
     7201 aacttagttt taaatccaga aaattatgat gtaatggtaa tgccaaatct ttatggagat
     7261 atactatcag atttaggtgc tgggcttgtt ggaggtttag gtataattcc aagcgcaaat
     7321 attggtaaag attgtgcgat atttgaagct gttcatggaa gtgctccaca aatagcaggt
     7381 caaaataagg ctaatccaac agcacttatt caatcatcag taatgatgct tagatactta
     7441 ggtgaatatg aaaatgcaca aaaaatagaa acagctttag aaaaagtatt tcttgaagga
     7501 agtaaattga ctgtagattt aggtggctca gcttctacta cagagtttgc ggatgaggtg
     7561 tgtaagtata ttgtatagat tttatttagg gaaaaagtga aatatagatt gaaggaaaga
     7621 ggctttctca aaatagagat aagccttttt ttattggaat gataaaatat atttgatttt
     7681 atacaaaaga gagttcctca taaacgaaaa agttaatttt gggatatcca ttttaatttt
     7741 aaaattaatt gattaatacg caaagaaaga ttaaacaaat ttattatttt taattgaact
     7801 ttcaaccaaa atatggtaaa atttaaaaaa gttgaacttt caactaaaaa taaatatatt
     7861 gattatgtga aattctataa aacagaaact atttgacttt agtcatggta ggttcagtgg
     7921 aggtgtgttt gattaagact ttagatagta atatattaag agaagtaggt actttatcta
     7981 gagcagtaaa ttcaataaat gatattaagt acaaggaatt aaagttacaa aaaggacaat
     8041 ttacattttt gacaagaatc tgtgaaaatc ctggtataaa tcttgtggaa ttatcaaata
     8101 tgttaaaggt agacaaggct acaacaacca aggctataca aaaactaata aaggcaggat
     8161 atgtagataa aaagcaggat gagtttgata aaagaggata taatttaact ccaactgata
     8221 aatctttgga agtttatgag ttgataattg aagaagagaa tagaagtata gagatatgtt
     8281 ttgataattt tacagatgaa gaaaaacaag tggtaactaa gttattagaa aaaatgagca
     8341 aaaacgtaga aaatgaatgg tttaaagtaa agagatagta aataaataca ttaacttaag
     8401 gagatatttt tatgattaga aaagcgaata tgaatgattt agaaagcatt atgaaaatta
     8461 taaaatctac agtggaagaa atgaagactt ataataatac acaatgggat gaaaactatc
     8521 cattagaaaa agactttgtt tcagatatta agaaacaaga tttatatatt tatgaagtgg
     8581 atggagaagt agcaggattt atatgtctta attatgaaga gccagaagaa tatttaggct
     8641 taaattggtc atctaataaa aaagcaatgg taatacatag aatggctgta aatccaaact
     8701 ttagaaagac aggaatagca tctaagttag ttgattttgc tgaaaaatta gctgtagaaa
     8761 acaatgtatc atacttaaag agcgatacat attcaataaa ctcaaaaatg aatttattac
     8821 ttacaaagtg tggttttata aaaatcggag aaatgagttt tttaggaaag gagaaatctt
     8881 tctactgtta tgataaaata ttataaagag catttataat acaaggggta aataatagat
     8941 tttcagcatt agtaagggca taatataacc cagtatatgc tattatgaaa gggatgattt
     9001 atatgattgg atttctaaag aaaagaagaa gtattagaaa atacaaggat gttgaagtag
     9061 aaaaagaaaa attagataaa atattaaaag ctgctctttt agctccatca tcaaaggggc
     9121 ttagaacatg ggaatttata gtggtagatg ataaagaaaa gctaattaat ttatctcaat
     9181 gtagaactaa gggtggtgga tttttcttaa aaaatgcacc tttagctatt gtaattattg
     9241 cagataaaga aaaaaatgat gtttggatag aggatgcaag tatagctgct tcatacatac
     9301 aacttcaagc tcatgaacta ggtttaggtt cttgttggat acaagtaaga aatagaatgt
     9361 atgatgataa tatagaagct gataagtaca ttagagaaga attaaaagta cctagcaagt
     9421 acagtgtaga atgtataatt tctataggat attctgatga agaaaagaaa gcatacaatg
     9481 actctgattt agactataaa aaggttcatt ttaacaactt ttagtatata atatgttgta
     9541 ttaagacatt atattttttt ctattgttaa tatatactta aaattgtaaa aatatatctg
     9601 aattttacta acttatctag gggttatgta caaggatttt aaagataaat atagaaatta
     9661 atgtttataa tcgatacata acttgagtaa caaaatttaa ggaggttaaa gtatgggtat
     9721 atttaaaaca aaaatggatg aggactggaa agtaaactac attaaagagt ttaatgaaat
     9781 gagagattct tatgagagta aacttcaaaa aaaacagttt gaggtagata gtttgaaatc
     9841 agaattggat agactaagaa gctataaaaa tagccttaag ccaaaagaaa aacaaattac
     9901 tgatgatgat ataaataata taaagagttt aagaagagat ggcttaagct ataaagaaat
     9961 ttcaaatcaa acaagttgga gcaaagcaac tgtaagtagg gtattaaatg gactttacga
    10021 ttagcatata catggggtga tttaaattgg gatgaagtat agttacaaaa aatgcataat
    10081 agattctatt ttattgatgt ttataattca aatattaagg atgattttga attatgtact
    10141 tctaagtcaa tttgaattta ctcttgaaaa ttttaatatt ataaacttaa tttcttttac
    10201 attagttggt ttatcgttaa ttttattttt aaaagataat tctttgtata acaaggttag
    10261 aaatagaaaa atcacagaag cttttgaaga aaataaaaac aatatactta ttgaaaagtg
    10321 taagcttata ctttttgttg tggttttatc tttagcaata attgtaactt attgtactaa
    10381 gggatatgta ctttttaatg taactatgat gactttgtct gtattgatag ttccaatatt
    10441 tgaagagtta ttttttagag aatatatatg gaactatcta agtaatttta taaagtcaaa
    10501 gagtaaaatt atctgtataa catctatact atctggaatt tataatatag gatacataga
    10561 tgttataaga aattatgtaa tattgtataa caattcaagc tacacctttg aagttattat
    10621 ttcaaaaatt atgataggta ctgtttttgg tattgtactt ggattagtta agtataggtt
    10681 tagagatgta gggttttgta tattattgag gagcttattt gctatattta ttaggtagat
    10741 agtataatcg gtttataagg ttgttttaaa tttaatttta aatcaacctt attttatttg
    10801 tgttttataa aacacttata tattaataac taataaattt aataaagatt atttgaatat
    10861 ttttaaaata tataaatatg tattgacata taaatattat gaattacaat ttatataaac
    10921 taataaaaat taaataatat taatcaactt attgaatgga ggaataaaaa tgaaatttgt
    10981 gactttttgt agtagcaatg aagaaaaaat aggtgtattt aattcagaaa caaatagtat
    11041 atatgaaatt aatagtttag gattaagtaa attatatact gatatgaatg attttattga
    11101 aaacgtatct acaggagatt tagaaaaaat aaaaaacaat tcatttgaaa acgcaaagtg
    11161 ttataagtta gaagaagtta aattatgttc cccaattgta agacctaaaa aagatattat
    11221 atgtttaggt ctaaactata aagaccatgt caatgaaatc ccagatggag ttataaaaaa
    11281 cgtagttatg ccagattatc caatttattt ttctaagaga gcagacaaaa taattggagt
    11341 ggatgataag ataagcttac atggagattt ggtagagaag ttggactatg aatcagaact
    11401 tgcagtaatc ataggtaaag aaggtataaa tatatcaaaa gaagatgctt atgaatatat
    11461 atttggatat acaatagtaa atgatataag tgaaagagca cttcaagata aacatgttca
    11521 atggtttaga ggaaaaagct tagacacaca tacatctatg ggaccttgta tagtacataa
    11581 agaagaattt gaacatcctt taaaattaga tataagtagt gtagtaaatg gagaagtaag
    11641 acaaaattct aatacagagt actttatatt tgatatacct actataataa atgatttatc
    11701 aagaggaatg actctaaagc caggagatat aatttcaact ggaactccag caggggttgc
    11761 tatggggatg aatcctcaag tatatttaaa gcatggcgat atagttgaat gtaaagtcga
    11821 aggaatagga gttttaaaaa atatagttga ttaatggata aatatgccat ataattttat
    11881 tagtacaaat aatttaatta atataggatt gatcaaactt atgattaatt tattatagag
    11941 agggatgagt atatggcaag agaactagaa ttaaagtatg gctgtaatcc aaatcaaaaa
    12001 ccatcaaaga tttatatgaa gaatggagaa cttcctatag aagtattaaa tggaaaacct
    12061 ggatatataa attttttaga tgcatttaat agttggcaac ttgtgaagga attaaaagaa
    12121 gctacaggtc ttccagcagc aacttcattt aaacatgtaa gtccagcagg agcagcagta
    12181 ggggtacctt tatcagatac tttaaagcaa atatattttg ttgatgattt agagctttct
    12241 ccattagctt gtgcttatgc aatggcaaga ggagcagata gaatgtcttc ttatggtgat
    12301 tttatagcac tttcagatgt atgtgataag gagactgcaa ctattatagc aagagaggtt
    12361 tctgatggta taatagctcc aggatataca gatgaagccc ttgaaatatt aaaaggaaaa
    12421 cgtaaaggca actataatat tgtaaaaata gatgaaaatt atactcctga gcctattgaa
    12481 acaaaggatg tttatggaat tacttttgaa caaggtagaa atgaaatatt aataaatgaa
    12541 gaactattaa aagatattcc aactgcgaat aaaattttta cagatagtgc aaaacgtgat
    12601 ttaattatag ctcttattac attaaaatat acacaatcaa attctgtttg ttatgctaaa
    12661 gatggacaag taataggggt aggtgcaggt caacaatcac gtattcattg tactagactt
    12721 gcaggtaaca aagctgatac ttggtatctt cgtcaacatc ctaaagtatt aaacttaaaa
    12781 tttaaaaagg atataggtcg tccagataga gataacacta ttgatgtata tctttctgat
    12841 gattatatgg atgtgcttgc tgatggcata tggcaaaact tttttgaaga aaaaccagag
    12901 cctctaactg gagaaggaaa aagagcatgg cttaaaactt taacaggagt agcacttggt
    12961 tcagatgcat ttttcccatt tggagataat atagaacgtg caaaaagaag tggggtaagt
    13021 tttatagcac aaccaggtgg ttctattcgt gatgataatg ttatattaac ttgtaataag
    13081 tataacatag tcatggcatt tactaaaaat agattattcc atcattaaaa tactgaaagt
    13141 ataaagataa tatagattaa gtaaaaatct acaacaagtt ttgttgtaga tttttttatt
    13201 tgctgtataa ttttaaatag aactttattt ttagatattc aatttggagg ttgatatggc
    13261 tttaaatata agaaactata gcctattatg taatcaagga acagctaaat ttaatgaaga
    13321 tgtagtcggt ataaatccat ttggggcatg ggttttagat ggtgctacag gacttaatgg
    13381 aaaaaattta gtgtcaaatg aaagtgacgc aaggtggtat gttcagtggt ggaataggta
    13441 tttatataaa aatataaata aagaagaatc attaaagagt attattaat
//
LOCUS       DAEPOZ010000103        13474 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.103, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000103 DAEPOZ010000000
VERSION     DAEPOZ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13474
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.103"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            388..1818
                     /locus_tag="KR440_002147"
     CDS             388..1818
                     /locus_tag="KR440_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0699195.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            1921..3024
                     /locus_tag="KR440_002148"
     CDS             1921..3024
                     /locus_tag="KR440_002148"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBH0699196.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMTYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENKKFVAVLKEILNIKGEPSIG"
     gene            3039..3275
                     /locus_tag="KR440_002149"
     CDS             3039..3275
                     /locus_tag="KR440_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0699197.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEKACQEA"
     gene            3292..4341
                     /gene="vorB"
                     /locus_tag="KR440_002150"
     CDS             3292..4341
                     /gene="vorB"
                     /locus_tag="KR440_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBH0699198.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            4343..5083
                     /locus_tag="KR440_002151"
     CDS             4343..5083
                     /locus_tag="KR440_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBH0699199.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            5086..5625
                     /locus_tag="KR440_002152"
     CDS             5086..5625
                     /locus_tag="KR440_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBH0699200.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLLEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            5749..7848
                     /locus_tag="KR440_002153"
     CDS             5749..7848
                     /locus_tag="KR440_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HBH0699201.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKMIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWALDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMKKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNTKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(8115..8630)
                     /locus_tag="KR440_002154"
     CDS             complement(8115..8630)
                     /locus_tag="KR440_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HBH0699202.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(8722..9933)
                     /locus_tag="KR440_002155"
     CDS             complement(8722..9933)
                     /locus_tag="KR440_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBH0699203.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKCLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDFPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
     gene            10609..12051
                     /locus_tag="KR440_002156"
     CDS             10609..12051
                     /locus_tag="KR440_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBH0699204.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            12093..12446
                     /locus_tag="KR440_002157"
     CDS             12093..12446
                     /locus_tag="KR440_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699205.1"
                     /translation="MKKSNIFSIIFPTIIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            12637..13458
                     /locus_tag="KR440_002158"
     CDS             12637..13458
                     /locus_tag="KR440_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBH0699206.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKAYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIKKVKSNNIA"
BASE COUNT         5143 a         1579 c         2419 g         4333 t
ORIGIN      
        1 taaataagaa aaagatattt ttaatatctg gatgtagtat agaaatatct tgaataaatt
       61 attcttgaat aaaattataa aaatttaaaa tcactagaaa tatgataaaa taatttaaaa
      121 tacgcatttc aatgacattt caagacaaga aaataataaa aaacaaatct aaattaagcc
      181 taaatagctt tttagttaat tttagtagta aaaaataaaa cttaaaaagt gtttgacaat
      241 tatgaatttt aagaatataa ttaaattaaa attatacaca tataaaaata tactcctgaa
      301 tcaagaatga gatatttaag attcaaaata tatattatta tacgtgaata atgaaaagta
      361 agaaaatttt ataagggagg ggtagatgtg agtagtcaag aaagcaaacc aaaaggaaat
      421 cttatatcaa attttagaga tgatatgaga gaccctaaga aaagagcaat atacacatat
      481 tcaagttcta taaacttagc gtacaatttt ataggttact ttgtatcaac tgttacaaat
      541 atttttttaa ctgatgtggc aaaacttggt gtaattatgg caggtggtat gcaaattata
      601 caatctttca taaaaattgc tgtagctcct ataacaggta cagtatttga caaacaacca
      661 ttcaaaaaag gaaagtatta tccatggctt agatatgcac cagcagcgtt agcattaaca
      721 tatggaatat ttttcatagt tccattaatg aaaataagtc cagcattact tttacctttg
      781 atgacagctt tattattaat agcatcttgt gctcagcaag tagttttcac tataatttat
      841 gctatttacc ctgtagttgc taagacacct agagaccgag ttataggctc aactactact
      901 aatatattta aagaagttgg taaattctta gtaggttttt catatccgtt attattagtt
      961 ttatttgcag atgtattagg tggagaagga atgggttatt taggtaccta cttattcttt
     1021 gcagcaggat gccttttagt attttggttt tcttcaacag agataataaa atctggagca
     1081 gaagaagaag ttcttcaaaa agaagagaag ccaaagatta aagcaagtga aatggcaaaa
     1141 gctctattca ctaatgtacc acttatgatg gcattttgtt tagaatttct tatatgtata
     1201 agatctatag gattaggacc tttggctcca tattatttta aatatgtaat agaagatgaa
     1261 cgtggattag cagtattttt atcaatcatg cctttggtaa gtgtagcatt catgtttttc
     1321 gcaccagtgt ttataaaaat ctgtagagag caaaaactag cttcaataat ttctttttct
     1381 atatgtgcac tatgccactt attggttgca tggactcctt gggggaaaac tactttagga
     1441 gtaactatgt tgctggcaat tggtggaggt ttttcaaatg tagtatcaat aataaatctt
     1501 aatttctttg ccggttcttg tgattatggg cattggaaat caggaaaaga cttacctggc
     1561 ttaagtatgt cactatatcc agttgcaata caagtaggag tattacttgc aactactata
     1621 agaacagttt taatgaactc aatgggatat caagctgata tggtagtcac agaagcagtt
     1681 aaatcaggtt ttataaatat gatttcttat tctatggcaa tacctttaat aatagctgtt
     1741 gttatagcaa tactatatcc agtaagtgac aagaaattaa accaaataag agaagagtta
     1801 aatcaaagaa atgcttagtt aaaagaaagt taagtagatg gttaatttaa gctattaaag
     1861 taagtagacg caatagcgtt tatataaatc aaaatacata tacagagggg gcttttaaaa
     1921 atgatagaaa aattattgag aaataccgta aaagaattac atcaatatgt tccaggtgaa
     1981 ccaatagaaa aagttaagga aaaatatggt gtaaaagaaa taataaaatt agcttcaaat
     2041 gaaaatcctc taggaccatc accaaaagca atagaagcta tgattgaaat gctaaaacaa
     2101 ggtcaacttt atccagagcc agaagctaac gaacttagaa gaaaattagc tgaaaagcta
     2161 gatttaaaac cagaaaattt tattgttgct aatggtgcgg ataacgttat cactctaata
     2221 ggtgaggctt ttataaatag aggagatgaa gttatatatt gtaacccaac atttccatca
     2281 tatagaactg caactataaa aaatgaaggg ataccagtag aagtgccttt aacagaagat
     2341 tataagtatg atttacaagg tatattagat aagattacag ataaaacaaa attaatatgt
     2401 gtatgtaacc ctaataatcc aacaggaaca attgtggatg ataaagagtt agaagaattt
     2461 ttaaagaaag tccctgaaaa tattataact atattggatg aagcatatat agaattttta
     2521 acagttccta attatgtgga tggacttaag tatgtaaggg aaaattataa cgttatagta
     2581 acaagaactt tctctaaaat atatggtctt gcagggctta gagtaggata tggtattgct
     2641 aaagatgaga taataagaac tttatttaca gtaaaagagc ctttctctgc aaatcgtgtt
     2701 gctataagtg gagcaacagc agcacttgat gatgaagagt ttataaaaga aacatatgaa
     2761 ttaaatagag ttggaatgac ttactttaaa gaagagttta caaaaatggg gtttgatgtt
     2821 gtagattcac aatcaaattt cctttatgta gatatgaaaa cagatatacc taagttattt
     2881 gaagacttga aaaaaagagg gtttgtaata agaccaagtg ctacacatgc acgtgtaagt
     2941 attggaacta tggaagaaaa taagaaattt gtagcagttt taaaggaaat attaaatatt
     3001 aaaggagagc caagtattgg ataatagata gaaatattat gaaacggggt gtagatatga
     3061 acttaaaaat aaacgcagag agatgtaaaa gttgtgaata ctgcgttata tcttgtaaaa
     3121 aaggagcatt aaaaatatca agtaagataa ataaagaagg ttatgctcat gtagaagttg
     3181 atgagagtaa atgcatactt tgtggaattt gctatcaggt gtgtccagat aatgtttttg
     3241 aaataattaa agaaaaagca tgtcaagagg cttagaagga gttgggataa tatgaaaaag
     3301 atgtggaagg ggaaccacgc cattgcagaa gcagccctaa gaggaggttg tgaattttat
     3361 gcaggatatc caattacacc acaaacagag gtaatggaat ttttatcaca tagaatgtct
     3421 gaacttggaa gaacttttat acagtccgaa aatgagatgg ctgctatata tatggtatat
     3481 ggagcatatg caagtggaat gaggtctatg acgagttctt caggaccagg aatatcttta
     3541 aaacaagaag ggatttctta cttatgtgca aatcattacc cttgtgtaat cgtaaatgta
     3601 cagagatggg ggcctggtct tggctcattg gactcagcac agacagacta tctaagagat
     3661 actagagggg gaggaaatgg agattatcat ttgatagttt atgcaccaaa cagtattcaa
     3721 gagactgtag atttgatgta taactcattt gatgtagcgg aaaaatacag agtgcctgtc
     3781 gaaattttga cagaagcagc tttaggtcaa atgatggaac cagtagaatt tccagaattt
     3841 aagaagagag aaggagattt aggttggact tatgatggaa gtaatagaga ccatgcaaag
     3901 gttgcagata accaaaagcc aactttttgt atggagaaac gtatgagaat aactgaaaat
     3961 gaacaacaat gggaagatta tcaagtagaa gatgcagaat atgtgtttgt agcgttcggt
     4021 ataccatcaa gaacaactat gaatgctgtt agaagactta gagaacaagg tgaaaaagtt
     4081 ggaataataa gaccaataac agtatggcct ttcccatata aagcatttga aaaggtaagt
     4141 aaagatgtaa aaggatttat ctcagtagaa tcaacagaca caggtcaatt agtagaagat
     4201 gttgctttag cctcaaagaa agtatgtaaa gaaaatgtac ctgtgtatgg tttatttagt
     4261 ggaaatcata taccaaaaac attgcaagta atggacactt atagcaaaat aaaatctggc
     4321 gaaatcaagg aggtgttcta atatggctac agaaaaatta acacctagcg ttgtttcaat
     4381 accaaacaag ttttgcccag gttgtggtca tggaatagta aatcgtataa tagcagaggt
     4441 aatagaagaa catggatatg aaaaaaatca tgtattaaca ttaggagttg gttgtgtctg
     4501 caatatgaat tttagctgga atggtgataa gatgcaaact gcacatggta gagcttcatc
     4561 tacagctata ggagttaaag tagcacttcc agatacatta gtaatgactt accaaggtga
     4621 tggagatgct tatgttatag gtctttctga aactttaaat acagcatata gaaatcataa
     4681 tgtgactgta tttgtaataa acaataataa ttttgcaatg actggtggac aaatgtcatg
     4741 gaccactatg ccgggtcaag tcacaacaac aagtgtaaat ggaagagata taaagactac
     4801 tggtagtcca ctgaaagttc ctgagatggt ggcaaacttc tttgatgtag catatgtagc
     4861 aagaggttca gttcatagcc caaaggaaat aattaatcta aaaaaatata taaaaaatgc
     4921 aatagaagct caattaaatg gagagggata ttccttagtt gaaatattag caccttgccc
     4981 aactaactgg ggtgtatcac tagagaaatc tatcaaatgg atggaagaag aaatagtgcc
     5041 atattatgct cttggcgaat ttaagcaaag ggatggtgaa tagttatgga aaaagagcta
     5101 gtatttgcag ggtttggtgg tcaaggagtt ttaactcttg gtctgattgt agctgagtca
     5161 gcacttgaat tggggaaaca agtaacttgg atgcctgcat atggtccaac tatgagaggt
     5221 ggaaaagcct atagtgtagt aaagttttct gatgagagta taggtgggcc tgatatggaa
     5281 gaaatagatg ttttagtagc aatgaataaa ccatctttag attatataaa tttagttaaa
     5341 gaaggtggaa cagtagttat taatacatct gcaatagatg agaatgtaac attaagagaa
     5401 gatataagtg tagttaaaat aaattgtcag gaattagctc aaaaagtaaa caatccaaaa
     5461 gcagcaaata tagtagtact tggagcatta attgctaaaa ctgggttact tgaaaaagaa
     5521 ttagctttaa aaactatgtg tgatttcttt gaggaaaaag gaaaaggaaa gtttaatgtt
     5581 caaaatgaag cagcattcat ggaaggctat aataacgcca tttgataaat tacccttatc
     5641 ttaagtgatt tatctcaaga tttaataatc tttagtgcca agagaaactt catcttgaga
     5701 taaaattcac ttaaaatcac aactataaac atataggagc gtgaatttat gagtttggaa
     5761 aaacttatta gaccaaaaag tattgctata gtaggagtga ctgataaact tggttttgga
     5821 agaagtgcag cacttagtat agtaaaaagt aaggaaacag atagagtata ttatgttaat
     5881 cccaaaagag aagaattgtt tgggagaaaa tgttataaga tgatacaaga agttccagaa
     5941 gtagtagact gtgtagtagt atgtacgcct agaaatgtag ttccatctgt attaaaagat
     6001 tctggagagt taggaacaaa ggcagcagtg gtatatgcta gtggatttgc agaggaaggt
     6061 acagaggaag gtacagattt agaaaatcaa cttatagaaa tatcaaatac atacgatatg
     6121 aaaatattgg gtccaaactg catggggttg ctaaattgta tagataaggt taatctatgg
     6181 gcaggaggtt ctaaatgggc tttagatact aaaaaacctg gtataggaat tgtaggtcaa
     6241 agtggattta taactgctga aattgtttca agtgattatt ttaatatatc ttatggtttt
     6301 tcaacaggaa atggaaatat agttacacta gaagatgcag tagatttcct tgtagatgat
     6361 gattatgcat cagtaatagc aatttacctt gaaggtttaa aaaatcctca aaaatttata
     6421 gatgctttaa aaagggcagc acaaaaaaga aagccagtaa taatattgaa atcaggaagg
     6481 tcagaaaaag gtgctatttc tgctgcctct catacaggaa atttagctgg ttcgagtaaa
     6541 gcttttgaaa gtatttttga aaaatatgga gtaatttctg tggaaaattt agaacagttt
     6601 atgtgtttag cacaagcttt tagtgtttta gatggtaatt tacctgcaaa ttctaatttt
     6661 gcagcaataa acttctcagg aggagaaaat actatttgtg cagatttagc agaagaaaat
     6721 ggtgtagaac ttgcagaaat atcttctgaa actaaagaag aaatgaaaaa atatcttcca
     6781 ggatttgcaa cacctaaaaa tccactagat gctacaacag cattattcca tgaaaaggat
     6841 atgatagtag gtttattaca tacctttgaa aaggatgata gtgttggatt tactatggta
     6901 ggtgcaaata taagagatga agaaaatgaa atgcatgaaa ccctttgtaa agctgtaagt
     6961 gaagctagag aacaaggatt gaaaaaacct gtgtttgcag ttcctacact tgaaggaaat
     7021 agatatttag attataggac tagattagaa gataatcaag tgcctataat gtcatcagtt
     7081 ggaactacat ttacttgttt taataaaatg gctaagttta tagattatga ttattcaaaa
     7141 agaactttag aatttaaagc tgttaagaaa agagagtcaa ataatgtagt agcattatca
     7201 gaattagata gtaagattga gatgaaaaaa tatggagtac cagttccagg acaaggtaat
     7261 acaaaaagta ttgatgaatt agatgaaatc ttaaaatata ttaagtatcc tgttgtttta
     7321 aaaataaatt cttctgaaat attgcacaaa tctgatgttg gaggagttaa aataggaatt
     7381 aagaatagag atgaggcagt agatgcatat aatgagattt taactaatgt aaaaaaagca
     7441 aaaccagatg ctaatattga tggtatatta gttcaagaaa tggtagaaag tggtatagaa
     7501 attattattg gtataactaa tgatgaccaa tttggaccaa tgttattagt cggtttagga
     7561 ggagtatttg tagaggtatt taaagatact acattatatc cattacctat aaatcatgat
     7621 gaagctatta tgatgcttaa aaaactaaaa tcatttaagc ttttaaatgg ctatagagga
     7681 agtgagcctt gtgatattga tgctcttgca gatatgatgg tcaaattagg aaaatatgct
     7741 tatgaaaata aggatgaagt caaggaaata gacttaaatc cagtgtttgt atatcctaaa
     7801 ggaaaaggcg tatgtgcagt agatgcctta atagttaaat ataaatagtt tataagtttt
     7861 ggtattcaac cataattaaa gcaccgactg aaaatcacat tttgtaagtt agatagtgtg
     7921 attttcagtc accccaaatt taatttaaaa atattttaaa atgaaagtat agttttgagt
     7981 ttaattgtga aatagcactg tataaacaat aaaatactac gtataaaacg aataataaaa
     8041 ggtgttttaa tgataagaat caatcttgat tttcattaaa acaccttctt tattatatta
     8101 aaaattttta aacgttatat tgtcacaggt gggtttgttg gagctgcata tgtacgatta
     8161 gcaaattctt ggtcaacaaa gaatagacca ccctctccag caagtttaac acgttttata
     8221 ttgtcttcac aatttgtttc ttcttctgct tgttcagata taaaccaaag taagaattga
     8281 gaagttctaa agtctgattc agagtttgct acagctgcta agttgtttat taaagctgta
     8341 accttttttt catgagctaa cgtacgctca agaatatcca ttgcagaagt aaagtctgaa
     8401 tctggcattg ggatagcatc aagctgaacc tttcctccta cattatgtaa gtatttgtaa
     8461 aaaaacatag cgtggtctgt ctcttcttta tattgaacat aaaaccaatt agaaaatcct
     8521 ttaagtcctt ctgcttcaag gtaagaagac atagaaagat aaagatacgc agaatataat
     8581 tcatggttaa tctgctcatt aagtaatttt tccattttag ctgaaatcat ttttatacct
     8641 ccaaatttta aatgctctta taaaatataa taatattaaa tggaaaaata ttcaataaaa
     8701 gtgtttaaat attttacata tttaataaaa atttatacta ccattaaaaa aattataact
     8761 tacaatggct atagagccaa ttaatggtgc atttgctcca aaatttgaat gccttacact
     8821 tattttttta tatttagaaa aacttgctga agcagtcaat ttttttaata atgtattttc
     8881 tataaatgta ccaggtatat taaagtcata gcctattatt atagaactac aatcaatcaa
     8941 tgtaagtgta ttaactaatg catatgatac gtatgtacaa aattcatcaa gcaaaaacat
     9001 ggcaagacta tctttattat ttgcctcatc tattaacttg actaaagaaa aatctttaca
     9061 gttaattaat ggggagttag gataaatatg gctcaatgat tcaattcttt taattagatt
     9121 actgacactt gtataataat ctaaacatcc attgttacca caactgcaca atggtccact
     9181 aaaattaatt gatgtatgtc ctatttctcc actttgtcct aggttgccag tatgaagttt
     9241 attttctaga actagaccag ctccaatacc attcattatg tgtaggtata tataatttga
     9301 tatattttta cctaaaccat acattttttc tgctaatgca cccgaattag catcatttat
     9361 caaaaatatt ggaaaatcag acatttcttt tataaaatct actatattta ggtttgatac
     9421 atttccaaag tctgggggat ttagtatggt accatttata tcatctactg gacctattga
     9481 agaaatacca attccaatta tatctctctt gttgattttc attattttag taaacattct
     9541 ttttattata tcttttaaaa catcatttga tagacattta gggtaagttt cagaaagttg
     9601 ttttacaata ttacctttta aatctgaaat aattacctta catatatttc ttcttattag
     9661 gattccacaa atacaaggcg atatattaga aatatctaat aaaataggtt tacgacctga
     9721 tgaatgtgca tttaaagttg atgactctat ttccctaatc atattttcat taataagttc
     9781 agtaactaga tttgataaag tcattttagt aagtcctgta gacttagcta aatcaactct
     9841 ggacataggt gtatttgtag aaagtaattt caatattatt aacttatttt taatttttac
     9901 atctttgatg ttagaacctt gtttgttact catcataaat ctccttaaat aaagttaagt
     9961 gttttattta ttttcaagta tactatcacc ttataataga gtaacatttt tttacttgaa
    10021 aatatcatta attagaatat atcacattat atatcataaa acatataaaa aaatgttaga
    10081 attaaattaa aagtgaaaag tagattgata tataaatagt aaatacttaa ttaaaaaaag
    10141 ataatgatag caataattat tgttaccaag aatctactac ttcaagtgtt accttaggta
    10201 tttaagttga tagtggtttg cacgtaataa acttgatgta atatatattc ttaataatat
    10261 cgataaaaga tttacttaaa aataatatag attatgtaaa catataatct atttattgta
    10321 tacaataaaa tttcattttg taaaacagtg aattaaatat gtaatataga tatcgatttt
    10381 tattacaaat tgttactaaa tataggtaca ttagaacaaa aatctagtaa attgtaacta
    10441 aaatgtaaat ttttccttta atctatacaa tattatggaa actttttgta atttatgata
    10501 taataacaaa tatataatta ttaaattata ataaatgact atagcgtaga tgtaatacta
    10561 tgttatagtc atttttgaat taaacttgat atatcaaggg ggaagaatat gagaacaatt
    10621 agaagagttt tagcattagg tttaacatta gctatatttc taataaatgt acctaatgta
    10681 gatgcattaa catcagatac aattaaaggt aacaatatat atgagacggc tggattaata
    10741 gcagataaaa agagttatga tacagcaatt atggtaaata tggataattc tatagcagat
    10801 ggtctttcag caagtggtct tgcaggtgct gttgatgctc caattttgct tgcacaaaaa
    10861 aataagatac caaatgaaac aaaacaaaga ttaaaaaatg ttaaaaagat atacataata
    10921 ggtaaagaat tatcaataag taagtcagta gaaactgaac taaaaaacac aggggcacag
    10981 gtaactagat tgggtggaga tgacagaata aagactagtt atagtgttgc taaagaggta
    11041 aatggtatca aaaaagttga tgaagtgata ttaactaatg catataaagg agaagcagat
    11101 acaataagtg ctgcacccgt atcagtaaga gatatagctc ctatagtact tacagatgga
    11161 aagagtgtgc ctttttcaac aagcaatgta aaaacatatg cagtaggtgg gagtatatca
    11221 atgagcacta gtttagttaa taaaactaat gctaaaagac ttggtggttc agatagatat
    11281 gatacaaata agaaagttat aaaagagttt tatccagatg catcagaatt ttatttgagt
    11341 gatggatatg atttagtaaa tgcacttaca ggttctacaa ttgctaagga aaatccaatt
    11401 gtattggtgt cagaaagtag tgataagtct atattagcag gagcagataa gattactaga
    11461 ttaggttcaa taagtgacag tgtgtataat aagtgtgttt ctgctgcaca aaataatggc
    11521 gattcgtcta caaaaggtga gtcacctatg aaaaatgaga caagcatatt aggtcagcca
    11581 acagctagtt tagaagcgtg cttaaaatgg gcaaaatcta aaaaagctaa tgatttattt
    11641 atagagttaa taccaatatt atatgatact gctgttcagg aaggtgttaa tcctgttttg
    11701 gcagtagctc aatctgcaaa agaaactggt ttttgtaatt ttggtggagt attagatgca
    11761 tcatttaaga atccttgtgg acttaaaact tctgtaggtg gttctgatac tgataagaat
    11821 gcccattcaa gatttgatac ttgggaagaa ggaatattag ctcaaattca acatttatgc
    11881 ttatatgcag gacaagatgg ttacccactt tcaaatccag tagaccctag acatgaaaaa
    11941 tctttatttg gtaaggctaa aacagttgaa agtttatcta ataattgggc tggaggtcaa
    12001 tatggacaag atttagtaag aatgatggga gaaattgagg caacaaaata attgaagatt
    12061 atcaaatatc caataaataa ggattgatta ttatgaaaaa gagtaatatt tttagtatca
    12121 tatttcctac aatcataatg gtgctaatgt ttttaacttt tgctacagat atatttaata
    12181 ttccagatat tcaatctaaa agtatttttg ttgtgggaat ggttttgatt tttccaattt
    12241 cttttttaat acaagggata atttgtgtat taaataagac taattggatt ttatctctta
    12301 ttgtgtcttt agtaacttat atagtattaa tgtatatatt tttaaatgat tctgcatata
    12361 tatatgtttt actttataca gctttttata tgattggaca tataacagct aaactctatt
    12421 acaaaattaa gacttttaaa aattaaattg aagtatataa aagtaacatt aagagaagta
    12481 tccttaatga ttttctaaaa tcagaaatag gtgcttctct tttttgtaaa aataaaagta
    12541 tgtaaataaa atttaaccat tataattttc ttttaacaaa tgtatacaat aaacttgttg
    12601 tataattaag atagataaaa taaagggagg gataatatga atttaataga taatcatatt
    12661 catactaatt tttcaagtga tggaaaggat tccatggaag atacaataaa aaaagctata
    12721 agtattggtg ttagatattt gacttttaca gaccatttag aacatgatga agatagagga
    12781 ttttgcataa attataatga ctatgttcca gtatttaata agtttaaaga aaaatataaa
    12841 aaggatatag agttattact aggtgttgaa gtaggttaca gaaaacatct aaaaaatgaa
    12901 atagaagaaa taataaattc taatcctttt gattttgtat tgtgctcaac tcatacaatt
    12961 gacaatgtac ctgttccatc aaaggcgtat tttaaaggtc ttagcaaaga agatgcgtat
    13021 tataaatatt ttaatagcat acttgaaact aatcgtgaat ttggagatta caatatatat
    13081 ggtcatttgg attatatatc taggtatgga atatattcag ataatagagt tatatacaat
    13141 gactttaaag atgttattga tgaggtctta aagtcaataa tcaataatgg gagtggtata
    13201 gagctaaata cttctggtta tagatatgga ttaaatgcta tacatccaaa tgaagatatt
    13261 ctaaaaagat ataaagaact tggaggattt atagttactg taggttctga ttctcatagg
    13321 gtagaagata tatgtaaaga ttttgatgtg gcctatgata tgcttaagta tcttgacttt
    13381 aaatatgtat ctttatttaa agaaagagaa acttattttg taaatataaa aaaagttaaa
    13441 tctaacaata tagcttagag tatgttttga agta
//
LOCUS       DAEPOZ010000104        13450 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.104, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000104 DAEPOZ010000000
VERSION     DAEPOZ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13450
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.104"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            301..1704
                     /locus_tag="KR440_002159"
     CDS             301..1704
                     /locus_tag="KR440_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699207.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            1731..3275
                     /locus_tag="KR440_002160"
     CDS             1731..3275
                     /locus_tag="KR440_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBH0699208.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            3306..4697
                     /locus_tag="KR440_002161"
     CDS             3306..4697
                     /locus_tag="KR440_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HBH0699209.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            4890..6386
                     /gene="abfD"
                     /locus_tag="KR440_002162"
     CDS             4890..6386
                     /gene="abfD"
                     /locus_tag="KR440_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HBH0699210.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            6521..6796
                     /locus_tag="KR440_002163"
     CDS             6521..6796
                     /locus_tag="KR440_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699211.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            6820..8127
                     /locus_tag="KR440_002164"
     CDS             6820..8127
                     /locus_tag="KR440_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBH0699212.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVRESLAVEFEKRFKEKY"
     gene            8147..9265
                     /locus_tag="KR440_002165"
     CDS             8147..9265
                     /locus_tag="KR440_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HBH0699213.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
     gene            9552..10472
                     /locus_tag="KR440_002166"
     CDS             9552..10472
                     /locus_tag="KR440_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBH0699214.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFIPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            10587..11744
                     /locus_tag="KR440_002167"
     CDS             10587..11744
                     /locus_tag="KR440_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBH0699215.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(11829..13328)
                     /locus_tag="KR440_002168"
     CDS             complement(11829..13328)
                     /locus_tag="KR440_002168"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBH0699216.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
BASE COUNT         5199 a         1620 c         2524 g         4107 t
ORIGIN      
        1 tttgatattt tcaatctatt aagaatagtg ctattattaa attaacaaaa gagaatagct
       61 ggtatggttt agagtaaatc aatctatatt acagttatat caacgaatat caaaaaatta
      121 tctattagaa aataaaaatt atttttacaa ttaaattgtt aattaaatca taaagaaaaa
      181 agtgatatta acaagataaa atttttttaa tcagaaatgt taatttaata acaaaataga
      241 gtattgggga gaaaggagaa atatattttt taataatatt tgttaattta ataacgaaaa
      301 atgaaaggga gtgatttagt gaagtttgaa ttgatggatt ttttaatgaa tccttttgtc
      361 cttatgtttg ctgcagtaat tacaggaatt ttatttggaa aaattaaatt tgggaagttt
      421 aattttgggg tatcaggagc attatttaca ggattattta ttggatggct tgcatatagc
      481 ttggggaatc taataatagc aaaaggagaa acagcagcag gatataaggc agcaactgta
      541 atgatgggga atggaattat ttcttctgac ttctttgatt tctttttaat aatatttgtt
      601 gcagcagttg gtctattggc agcaaaagat atgaaagctg tattaaagaa gtatggagca
      661 agatttgtaa ttttaggagt tcttataact tttataggtg gctctatgac ttatgcaatg
      721 actttactat caagtgataa aggaagtagt gcttatgaag tttctggcgt ttacacagga
      781 gcattaacaa gttcgcctgg attagctgct gctttagaaa cagctggtaa acatgcagaa
      841 gatgtatcaa aagaatttga aaaagcttca ataaaagata aaaaagaaat tttaaaagta
      901 gtcgacccag aaggcaaatt ggatgtgaat acaactacat cactgactca agagcaaata
      961 gataagtata ttgcatatgc tgaagctggt gttggtatag gtcatgcagt cgcatatcct
     1021 tttggagttt taatagtaat cttaggtgta aatttccttc caaaattatt taggatggac
     1081 ctaaaagaag aaagaagaaa atatgagaag gaaatgaagg aagcaagaga tagtgtttct
     1141 ggtaaaacga taccagaagt tccatttaat ataataacat ttttcttgac atgtttagct
     1201 ggatacttag ttggaggaat acatgtattt atgggaccac tagggtactt cacactaggc
     1261 gcaacaggag gttcactaat tgtatctcta gttcttggat atataggtaa gataggggta
     1321 gtaaatttca gaatggaaga aaaggtttta aatattttaa aacaaatagg gctagtattt
     1381 ttcttagcaa tagttggatt aagatatggt ggaaaagtag ttgattcaat tatgacatct
     1441 ggtatgcacc tagccttagt agcaatagca gtaggtgtta cagctatgat gataggattc
     1501 ttagttggca aatacgtatt taaattaaat tggattttat tatctggagc tgtctgtggt
     1561 ggtatgacat caacaccagg tcttggagct gctgttgatg ctcttgatag tgatgaccca
     1621 gcagcaggtt atggtgctac gtatccattt gcattattaa ctaaagttat attggttata
     1681 gttttacaca aacttccaat gtaaataaga ttttatatgg ggggatattt atgaataatg
     1741 aaggagcatt tgaagaatta agtggaagat taagaaataa agaattgtgt gatttaataa
     1801 tgaatgcaga tgaagctgct aaaattatag aggatggcat ggttattgga gttagtggat
     1861 ttacaccttc aggctatcca aaagcagtac cattagcagt ttcagaaaga gctaaaagtg
     1921 gagaaaagat aaaattaacc gtatattcag gagcatcttt aggcccagaa gttgatggag
     1981 catggtcaga agcaggtata attgaaagaa gacttcctta tcaaactaat tcaatactta
     2041 gaaataatat taataaaggt gttgttgatt atatagatat gcacctaagt cattctactc
     2101 aatttttaaa ttatggaact atacctaaag tagatgtagc tatagtagaa gcacttgcaa
     2161 taactgaaga agggaatatt ataccaacta gtgggatagg taattctcca tcttttataa
     2221 aaagtgcaga taaagtaata gtagagataa atcttgcaaa accaatggaa atggaaggta
     2281 tggcagatat atatattacg gaaaatccac caaatagaaa accaatagaa ataaatcatc
     2341 ctaaggatag aattggaaca acatatatac cttgtggttt ggataaaatt gcaggagtag
     2401 taattaccaa tatgcaagat aagacccgtc cattgggggt tgtagatgaa gcttcgaaaa
     2461 aaatatcaaa taatattatt gcttttttaa gagaagaagt aaaggcaggc agattatcaa
     2521 agaatttact tcctcttcaa tctggtgtag gaagtgtagc aaatgcagta ttatatggat
     2581 tatgtgaatc tgaatttgaa aatttaactt gttatacaga ggttgtacag gactctatgt
     2641 tggatttaat aagaatgggt aaagttacaa tggcatctac tacatctgta agtccatctc
     2701 cagaaggatt aataaaattt gaaaaagata ttgatttttt taaggataag attatattaa
     2761 gacctcaaga aattagtaac aatccagaaa tagccagaag aattggtgta attgcaatga
     2821 acactgcaat tgaagttgat atttatggaa atgtaaattc tactcacata atggggtcaa
     2881 agatgatgaa tggaattgga ggttctggtg attttgctag aaatggagca ataactatat
     2941 ttagtactga gtcgattgca aaaaatggtg atatatcatc aatagtacct atggtatctc
     3001 acgtagacca cactgaacat gatgtaatgg taattgtaac agaacaagga tatgctgatt
     3061 taagaggtct tgcacctaga gaaagagcta taaaaataat agaaaattgt gcccatccag
     3121 attataaaga acaacttagg gattatttaa atagagcttg taaaagtgga gcaaaacaaa
     3181 cacctcacat attggacgaa gctttatcat ggcatagtaa atttatgagt acaggtacaa
     3241 tgaaaaaggc tgaaactttt aaaagtgcat tataaaatat ttaataaatt ggaggtagat
     3301 tttaaatgga aaaagcagtg gaaaactttg aagatttaag taaggaatac attaatggtt
     3361 atattgagag agcaagaaaa gctcaaagag agtttgaatg ttatactcaa gaacaagtag
     3421 ataaaatagt aaaaatagtt ggaaaagtag tgtattataa tgctgaatat ttagctaaac
     3481 ttgctgtaga agagactgga atgggagtat atgaagataa ggtagctaaa aataaaagca
     3541 aagcaaaagt tatatataac aatttaaaag ataaaaaatc agttggtata attgatatag
     3601 atagagaaac tggaataaca aaggtagcta aaccagttgg agtagtagcg gcaataactc
     3661 cttgtacaaa tccaatcgtt acacctatga gcaatgcaat gtttgcactt aagggaagaa
     3721 atgcaattat tatcacacct catcataaag caattggatg tagtacaaaa actgtagaga
     3781 tgattaatga agaattagaa aaaataggag ctccagaaaa tttaatacaa atacttgacc
     3841 aacaatcaag agaaaataca agaaatttaa tttcatcagc agatgtagta attgccactg
     3901 gaggaatggg aatggtaaaa gctgcgtact caagtggaaa accagcactt ggtgtagggg
     3961 caggaaatgt tcaatgtatc atagatagag atgtagacat taaagaagca gtacctaaaa
     4021 tcatagcagg aagaattttt gataatggta tcatttgttc aggtgaacag tcagtaatag
     4081 tagcagaaga aatgtttgat aaaataatgg atgaatttaa aaataacaaa ggttttatag
     4141 ttagagataa agtacaaaaa gaagctttca gaaatgctat gtttgtaaat aaatctatga
     4201 ataaagatgc agtaggtcaa tcagttcata caattgcaaa aattgcaggt gttgagatac
     4261 cagaagatac aaaaataata gtaatagaag ctgatggacc aggagaagag gatattattg
     4321 caaaagaaaa aatgtgtcca gttatatctg catacaagta taaaagtttt gaagaaggtg
     4381 tagctattgc aaaagcaaat ttaaatgtag aaggaaaagg acatagtgta tctatacatt
     4441 caaatacagt taaaaatata gaatatgcag gagaaaatat agaagtatca agatttgtta
     4501 ttaatcaatg ttgtgctact agtgctggag gaagtttctt taatggtctt gcacctacta
     4561 atactctagg ttgtggttct tggggaaata acagcatctc tgaaaatctt gattataagc
     4621 atctaattaa tatttctaga attgcttatt atatgccaga aaatgaagtc cctacagatg
     4681 aagaactttg gggataaaac ttagacaaaa aatattcatt ctaaattaaa gtaggaatat
     4741 attttaattt agaatgaatt taagacataa attaaattta aattagttta ttggactaaa
     4801 tttaaaatat taagagacat atagaagaag gaagtattat aatttaaaat aaataaaaaa
     4861 atctataaaa gagaaggagt ggttttatta tggcattaat gacaggagct caatatattg
     4921 aaagtttgag aaaattaaat actaaggttt atatgtttgg tgaagaggtt aagaattggg
     4981 ttgaccatcc tatgattaga ccatctataa attgtgtagc agcaacttat gacttagctc
     5041 atgacccaga atatgcagat ttaatgacag ttacttcaaa tatcacaggt gaaaaaataa
     5101 atagatttgg tcatcttcat caaagtgttg atgatttgat aaaaaaagta aaaatgcaaa
     5161 gactatgtgg tcaaaaaaca gcttcttgtt tccaaagatg tgttggtatg gatgcgttta
     5221 atgcagtgta ttcaacaaca tttgagtgtg ataaagccca tggaactaat tatcatgata
     5281 attttgtaaa atacttaact tatatacaag aaaacgattt agtagttgat ggagctatga
     5341 ctgaccctaa gggagataga agtctatcac ctagtgctca acctgaccca gatatgttcc
     5401 ttcatatagt agaaagaaga gaagatggga taattgtaag aggagcaaag gcacatcaaa
     5461 ctggttcaat aaattcacat gagcacctta taatgcctac tataagtatg actgaagcag
     5521 ataaagacta cgctgtatca tttgcagttc cttcagatgc tgaaggtgtg tttatgattt
     5581 atggaagaca atcttgtgac accagaaaat tagaagaagg tgcagatgtt gatttaggaa
     5641 ataaggagtt tggaggtcaa gaagctttag tagtatttga taatgtattt gttccaaatg
     5701 atagaatttt cttatgtgga gaatgggatt tctctggtat gttagtagag agatttgcag
     5761 gataccatag acaaagttat ggtggatgta aagtaggagt aggtgatgta ataataggag
     5821 cagcagcttt agcagctgat tataatggag caaacaaagc ttcacatata aaagataaac
     5881 taatagaaat gactcacctt aatgaaagct tatattgctg tggtatagca tgttcatcag
     5941 aaggtcacaa aacagaagct ggaaactatc aaatagattt acttcttgca aatgtgtgta
     6001 aacaaaatgt aactagattt ccttatgaaa tagtaagact tgcagaagat atagctggag
     6061 ggcttatggt tactatgcct tcagaaaaag actttaaatc agaccttaaa gttggaacaa
     6121 gtggaatgac tattggcgaa gtatgtaaca agtattttaa agcttcttca gtggcttcaa
     6181 cagaagaaag aatgagaata ttaagattct tagaaaatat atgtcttggt tcatctgctg
     6241 ttggttatag aactgagtca atgcatgggg caggttcacc acaagctcaa agaataatga
     6301 tttcaagaca aggaaacata aatcaaaaga aagaattagc taagaaaatt gcgggaataa
     6361 aaaaagagga agctttaaat ttataaaaag ttgatatata aagggaaatt atatattaat
     6421 gtgtattaat taatgaaaag atatgtagta taagagatag ttataaaatt atggtggttt
     6481 ttagctaagt aaagaaattt ttaataagga gaatttacac atgacctgtg gagttagatt
     6541 ttgtgggggc tgtaacccta gatacgaaag agggaaggct cttacaataa tcaaagagca
     6601 cttcaatggt agggtagatt ttagcatagc acaagagaac gtcgagtatg atttgctact
     6661 tataattgga ggatgcgcaa ctctatgcgc atcctatcaa caatataaag tgaaaaatga
     6721 atgtgttaag atgtgggatt ttagccatat agaaagagtt ataaatgtga ttgaatcagc
     6781 aatttgtgaa acttaaaaaa gctaaatggg gggatttcat tgagttggca agaattgtat
     6841 caaagtaaat tatgtagtgc tacagaagct gtaaaacaga ttaaaaacgg tgatactgta
     6901 gtatttgcac attgtgtagg tgaacctcct gcactagtag aggctatgat tgaaaatgct
     6961 gaacaatata aagatgttga gattaaacat atggtaagct tgggtagtgg tggatatact
     7021 gctaagggaa tggaagctca ttttagagta aatccaatgt ttgtaagtgg aaatgtaaga
     7081 aaggcaattg aaaatggaga tggtgatttt acacctgcat tcttccatga agtaccaaag
     7141 ttgcttagag aaaaaagatt aaaatgtgat gttgttttag cacaggtaac tccaccagat
     7201 gaacatgggt attgtagctt aggaacatca gttgattata cttatgaagc cattaaaact
     7261 gctagaacag taattgttca agtgaatgac cagtttccta gaacctatgg tgaggtagtc
     7321 catgtatcag agtttgacta tatagtggaa aaatcacaac ctttatttga actacaacct
     7381 gcaaagattg gagaagtaga agaagcaatt ggtaaaaatt gtgcttcatt aattgaagat
     7441 ggttctactt tacagctagg tattggtggg ataccagatg cagttatgtt atttttaact
     7501 gataaaaagg atttagggat tcattcagaa atgatttcag atgggacttt ggccctttat
     7561 gaaaaaggtg ttataaatgg taaatataaa aattttgata aagaaaaaat gacagttacc
     7621 ttcttaatgg gtactaaaaa attatatgac tttgcaaata ataaccctgc agtagaggta
     7681 aaaccagtag actatgtgaa tcatccagca attataatga aacaacataa gatggtttct
     7741 ataaattctg ctatacaagt tgatttaatg ggacaggtag ttgcagaggc tatgggactt
     7801 agacaattta gtggtgttgg aggtcaagtt gactttatac gtggagtgtc tatgggggaa
     7861 gatggaaagg caattatagc tatgccttca ataactacaa aaaaagatgg tacagtaatt
     7921 tcaaaaatcg tatctattgt ggatgaaggt gcacctataa cgacatcaag aaatgatgtt
     7981 gactatattg taacagaata cggtatagca gaattaaaag gaaaatctct tagagaaaga
     8041 gcaagaaatt taataaatat agctcatcca agtgtaagag aaagtctagc agtagaattt
     8101 gaaaagagat ttaaggagaa atattaataa tagaggggtg ttaataatgg aagcacttag
     8161 agtagttcca aagatatttt attttgatac atttaaagaa tttaatgaag aatttaaaat
     8221 aggaaaaaat gatttagtaa ttactaatga gtttatatat gagccatata tgaaacctct
     8281 tggaatagat acaaatttaa tttttcaaga aaagtttgga acaggtgaac catctgatga
     8341 aatgatagac tccatgacaa aggaaatgaa gaaatacaat tttgatagaa ttattgcgtt
     8401 tggaggagga acaatagttg atatatgtaa gatattagca cttgatgttc cagaaaagtc
     8461 tattgattta tttgaaggag aagtatcacc taaaaaagta aaagagttag tagtagttcc
     8521 tacaacatgt ggtacaggta gtgaagtaac aaatgtagct attgcagagt taaaatctaa
     8581 acatactaaa aaggggcttg cagtagaaga aacatatgca gactatgcag tgttaattcc
     8641 agagacagta aaagggttac cttacaaatt ctttgttaca agttcggtag atgctcttat
     8701 acatgctatt gagtcttatc tttctccaaa agcaagtcca tttacagaga tgtattctct
     8761 acaagcaata aaaatgatta tggatgggta taagaagata gttgataaag gagaagaaga
     8821 gagatttaat catcttagag actttgtact tgcatcaaat tatgcaggaa ttgcttttgg
     8881 aaatgctggt tgtgcagcag ttcatgcact atcttattca ataggtggag ctttccatgt
     8941 tgcacatggt gaagcaaatt atcagttctt tactgaagta tttaagatgt attctagaaa
     9001 aaaacctaat ggaaaaatta taaaatgtac aaaaatttta gcagatgccc ttgaatgtga
     9061 ccctaactgt gatgtttatg gagaattaga aaagttttta aataaattaa tagctaaaaa
     9121 agctttgaga gaatatggaa tggtggaaag tcagatagat gaatttacag atagtacaat
     9181 agcaaatcaa caacgactac ttgctaataa ttatgtagag cttagtagag aggaaataag
     9241 agagatattt gcaaatcttt attaatacaa ttttttattt atatgtatag tcaacataaa
     9301 caatgctaat tatatttatg tacaaataaa aataagggaa cttaaatttt aattaaagtt
     9361 aagttctctt atttttatat ggagttaaat ataagtatat ttttaattta aaatatgctt
     9421 atatttaagt ctattacttt atttgaaaca atttgtagaa tttcaaattc cttgacagta
     9481 ataagtaagt aattgtataa tcaaactgca atatattaat aatattagtg aaaatgaaag
     9541 gattataaaa tatgtataaa tcagaaaatt tttcaaaaaa atctgtatta ataaattcat
     9601 ttttaagtaa atttttaaaa ataattggat ggggtttggt aagcttattt acattatcag
     9661 ccttagtact ttcaactatt tccattataa ttggatgggc tgtaatactt cctattcaag
     9721 tatgtgcagt atttttactt ataggaggag tttgtatttt taaaggtaag agattaggag
     9781 accaaatttc tcgttataaa aaatattgta caattataaa caatagaaat attattccag
     9841 tagaacttat tgcagaaaac acatctaaat cattaaattt tataataaaa gatgtacaaa
     9901 agatgataga caaaaactac tttataaata cttatattga taaaagaaat aatcagattg
     9961 tgttaacaaa tgaagatttt attccaccaa gatatgaaga tgtagcatat gaagtcaaag
    10021 aagaagaaaa gactgatgaa tccaatgaag ttgatacaat tataaataaa ggaatgaatt
    10081 atctaacaca aataaaagaa gctaataaaa atattaaatc agaatcaatg ggcgataaaa
    10141 ttgtacaagt agaagatgtt acaagtaaaa tatttgatgt tgtaaaacat gacccatcaa
    10201 aacttactca aatacaaaaa tttatggatt attatcttcc aaccacttta aaactattaa
    10261 attcatatca tacattggaa gaacaaggaa ttgatagaga aaatataaca acaactatgg
    10321 aaagtattga aaatacaatg aatacgattg tagtagcttt tgaaaatcaa ttggactatc
    10381 tgtttgaaga tgaagccatt gatatttcta cagatattac tgttttagag aatatgttag
    10441 tccaagaagg attaacgagt ggtaaatttt aacttcaata gaaaaaatat tctgttattt
    10501 ttaaacaatt aattagtcat aaagtataaa atgtgtatta tataagttaa tcagtacttt
    10561 atgaaggaag aggagagaaa gatacaatga ataatttaga tgatatacca gttatgccaa
    10621 ctttgacact tgacccattt ggagaagaat caaacactaa tgatatagat aattctgact
    10681 tattaatgaa aaaggatgaa aaagacccag aagaagaaaa actgtctgaa tctgaacgca
    10741 agatggtaaa ggaatttgcg gaaaaaatag atattacaaa tacaaatatg attttgcaat
    10801 atggagctgg tgctcaaaaa aaggtagcaa gtttttctga aacagcttta aagagtgtaa
    10861 aaacaagaga tttaggagaa gttggagata tgcttacaaa tctagttact gatttaaaag
    10921 cattttcagc agatgaaaaa gaacaaagtg gttttttagg aatttttaaa aaggcaaata
    10981 ataaaatttc aaatttaaaa actaagtatg atagtgcaga ggtaaatgtg gataaagtaa
    11041 gtaaagagct tcaaaagcat caggttaaaa tattgaaaga tatagcaatg cttgataaga
    11101 tgtatgaatt gaacttagct tactcaaaag agcttactat gtatattata gcaggaaaac
    11161 aaaaattaaa ggatatgaaa gaatatgaaa tgccaaagtt gagagaaaaa gctcgtttat
    11221 ctggttcaac agaagatgct caatctgtaa atgatatggt ttcactttgt gacagatttg
    11281 aaaagaaaat tcatgactta gaattaacta gaatggtatc gcttcaaatg gctcctcaga
    11341 taagattggt tcaaaatagt aataatctga tggcagaaaa gattcaatca actatagtaa
    11401 ataccattcc attatggaaa aatcaaatag tgcttgctct tggaatttct cacttaaatc
    11461 aagctatgaa agcacagcgt gaagttagta atatgacaaa tgagctttta ttgaagaatg
    11521 cagaaacttt gaagatggga acaatagaaa cagcaaaaga atcagaaaga ggaattgtag
    11581 atatagacac aattaagaaa actaatcaat ctctaatatc tactattgat gaggtagtta
    11641 aaatacaaca tgatggtaga ataaagagac aagaagcaga agttgaatta tcaaaaatag
    11701 aaaatgaact taaaagtaag cttcttgaat ttacagttaa ataaaaatta tactaatata
    11761 aaaagagact gtctcaaaat agagataagt ctctttttca ttgtttaaat gattaaagta
    11821 tatttatatt ataaatttct attatctttt aacataacat catgagctcc accttcaact
    11881 atactagtag aagatacaag tgtaagttta gcattttttt gaagttcagg aatgctaagt
    11941 gctccacagt tacacatagt agaacgaact ttacttaaag aaagagttac attgtctttc
    12001 aaagaaccag cataaggcac ataagagtca acaccttctt caaaagaaag tttcttatct
    12061 ccaccaaggt catatctttg ccagtttcta gcacgagcag aaccttctcc ccaatactct
    12121 ttcatataac taccatttat attaactttg ttagttggac tctcatcaaa acgagagaag
    12181 tatctaccaa gcattataaa atctgcaccc atagctaaag ctaaagttat atgatggtca
    12241 taaacgatac caccatcaga gcaaattgga atataaatac ctgttttttc aaagtattcg
    12301 tctctagctt gagcaacttc aattatagaa gtagcttgtc cacgtcctat acctttttgt
    12361 tcacgagtta tacatataga accaccacca ataccaactt taacgaagtc agcaccagct
    12421 tcagctaggt aaaggaatcc ctcgcggtca actacatttc cagctcctac cttaactgta
    12481 tcaccatatt tttcacgaat gaaatcaagt gttatttttt gccactcaga aaaaccttct
    12541 gaagaatcta tacataaaac atcagcacca gcttcaacta aagcaggaac acgttcagca
    12601 aagtctctag tattaatacc agctccaact acatatctct tagaagaatc taaaagttca
    12661 agtggatttt ctttatgaga agagtaatct ttacggaata ccatatacat aagattacca
    12721 ttatcatcaa gtacaggaag agaattaagt ttgtggtccc atataatgtt gtttgcttcc
    12781 tttaaagtaa catctttgtt agcataaact atagatgaca taggagtcat aaattcacta
    12841 actttagtat ctaaatccat acgacttata cgataatcac gacttgcaac aatacccaat
    12901 aatttaccag ttgaagttcc atcttcagta acagctactg tagaatgtcc agttttttct
    12961 tttaagtcaa gtatgtcttt taatgtgtta tcaggtttaa tatttgaatc acttacaaca
    13021 aaaccagctt tgtgggattt aacacgtgct accatagcag cttcattttc aatagtttga
    13081 gaaccgtata taaatgaaat accaccttct ttagcaagtg caatagccat tttgtcatca
    13141 gaaacagatt gcataatagc agaagtcaaa ggtatattca tataaatatc tgcttcctcg
    13201 ccctttttaa atttagtaac aggtgttttt aaacttacat tagcaggtat acaatcagta
    13261 gaagagaaac ctggtacaag aaggtattca ctgaaagtat gtgatggtgt atcaaaataa
    13321 aacgccattt tttaatcact ccttatattt ttatactttt tataattgat agtattaatt
    13381 ataacttatt ctttaatttt attaaagtat tttgtcaaat attgagattt ttgaaaaatt
    13441 taaactattt
//
LOCUS       DAEPOZ010000105        13369 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.105, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000105 DAEPOZ010000000
VERSION     DAEPOZ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13369
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.105"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            343..1044
                     /locus_tag="KR440_002169"
     CDS             343..1044
                     /locus_tag="KR440_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBH0699217.1"
                     /translation="MNNSFLDKLIETKELKNSLYNVLKHNFLYHSNKIEGSTFTTEAL
                     ALLLDKNVVTGRHTLDDVQETVNSSYVFDTVIDSLKEKITHNFLRNLHSSLIFNTTLH
                     SRGMAGIYKTIPNMILGTDVSIAQPFEVEPKLDELIEWYYSQSEVSIKVIAEFHYRFE
                     LIHPFQDGNGRIGRFVMLKQMLENNLPIKIVSWDSEDLYRNSLNSCSLGNYVPLIEYL
                     SSLEDFREVYKMLWK"
     gene            1948..2598
                     /locus_tag="KR440_002170"
     CDS             1948..2598
                     /locus_tag="KR440_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0699218.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
     gene            2876..4942
                     /locus_tag="KR440_002171"
     CDS             2876..4942
                     /locus_tag="KR440_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBH0699219.1"
                     /translation="MKEVVESLKKITKERIENTMKKLFTSETFLEYQFISDCQISPDG
                     GYTAFIVKKADIKENGYTSQVYILNNKTGELKQITSINSVGAYVWEDENTILFPALRN
                     EKVKEAVKNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRP
                     IVEGLPEEEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYIYDSNINKLKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNICILEKDKYIIKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGA
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCL
                     GNGLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLAIHGGPRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGDIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSSMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIK
                     KDNIGQ"
     gene            4959..6914
                     /locus_tag="KR440_002172"
     CDS             4959..6914
                     /locus_tag="KR440_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBH0699220.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKAGTTDFYTISIEGGE
                     AKEAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDR
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGIDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLERSITQNHDIFILDLST
                     SEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAVSQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            6930..8120
                     /locus_tag="KR440_002173"
     CDS             6930..8120
                     /locus_tag="KR440_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBH0699221.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            9254..9628
                     /locus_tag="KR440_002174"
     CDS             9254..9628
                     /locus_tag="KR440_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0699222.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFVSTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMGILYEED"
     gene            9630..10487
                     /locus_tag="KR440_002175"
     CDS             9630..10487
                     /locus_tag="KR440_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0699223.1"
                     /translation="MENILEVYNLNKTYTNFSLSNISFVLPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMSEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTEYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDEIKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            10490..11128
                     /locus_tag="KR440_002176"
     CDS             10490..11128
                     /locus_tag="KR440_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBH0699224.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFILWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVMIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            12102..12836
                     /locus_tag="KR440_002177"
     CDS             12102..12836
                     /locus_tag="KR440_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0699225.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDRLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEVSYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMVLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELTPVDVMHSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRDIIGTLKNLIIE"
BASE COUNT         5063 a         1495 c         2251 g         4560 t
ORIGIN      
        1 taataatagc tatatatttt ttcttcttat ttataaattt aatgatttat agtcataaaa
       61 tgaactctaa gaagagataa aaatgcgttt tatatgtttt tagattaata tgaaatatca
      121 caatgaaatg ttgtacaaag tgatatataa taattatatg aactataaaa ttgactaaac
      181 ttattgacta ataaattaga tgaaaagttg agacttactg aaaataaata caatgaaata
      241 tatactacaa gtattaaatt aaatctgaac tttacatact attagaataa gtattataag
      301 attataatga tttaaaaata ttaaaataat tgaggtgata acatgaacaa ttccttttta
      361 gataaattga ttgagacaaa agaacttaaa aattcacttt ataatgtgtt aaaacacaat
      421 tttctttatc attctaataa gatagagggc agtactttta ctactgaagc attagcactt
      481 ttactcgata agaatgttgt tacaggaaga catactttag atgatgtaca agaaactgtt
      541 aattctagtt atgtatttga tacagtaatt gactcactga aagagaaaat aacacataat
      601 ttcttaagga atctacattc aagtttaatc ttcaatacaa ccctacattc cagagggatg
      661 gctggtatat ataaaactat acccaatatg atactaggaa ctgatgtaag catagcacaa
      721 ccatttgaag ttgagccaaa gcttgatgaa ttaattgaat ggtactatag tcagagtgaa
      781 gtgtctatta aagttatagc tgaatttcat tatagatttg aattgataca tccatttcaa
      841 gatggtaatg gtcgcatagg tagatttgtt atgctaaaac agatgttaga aaataatctt
      901 cctataaaaa ttgtatcttg ggatagtgag gacttgtata gaaactcctt aaattcttgt
      961 agtttaggta attatgtccc tttaattgaa tatttatcaa gtttagaaga ttttagagaa
     1021 gtatataaaa tgttatggaa ataattgttt gtagcaaaaa aagtttttat ataaatatat
     1081 ttttaactat gctatttaac gttgatttct tagttattct tagcttggac tatacccact
     1141 attttaacta tataaaatag tggatatagt ttaactatct ctttataact atttttatgt
     1201 atcttagttg tttcttaata ttgggacagt tcataaaact aaaagtgatt tttaatttta
     1261 tcatttataa tttaccatta catcagaaaa atgcttatca ttagaatttt cataaaaggt
     1321 acctattttc ttcaattaac taaatcaatt ctattagtct taatctttcg tatttcaaat
     1381 tgataataca agaaaataaa aaataaaaaa ttttaaattt tttatttatt gcgaaaaaaa
     1441 taaaaaggtg ctatactttt tgtaacatat tattcaaatt attaagagat agtaaatatt
     1501 tatagtttaa tttgtagtac actagcatga tttgcagaac aatgaattaa tctatagtat
     1561 aatagtttaa tttgaagtac aacgatttaa tctatagtac actagcatga tttgaagtat
     1621 aacgatttaa tctatagtac aataacttga tttgcaatac aataatttaa tctatagtat
     1681 aatagcatga tttgaagtat aacgatttaa tctatagtac aataacttga tttgaagtac
     1741 aacgatttaa tctatagtac aataacttga tttgcaatac aatgatttaa tctatagtat
     1801 aatagcatga tttacagtac aataatttaa attgtagtac aaaagtacta tcaaataaga
     1861 atttctactt taaaaatatt actaaaagta attaaaatcc aataattcaa tataatatgg
     1921 caaaagaata gaaataaggt gagagtagtg aaagtaaagt caaatttaga tgaaatttta
     1981 tatcataaga ttattgaaag tcttattcgt ggtgaatact ctgtaggtca aaaaatatta
     2041 ctaaatgacc tttgtgaaaa atttgaagta agcagaacac cagttgtaca agcagtaaaa
     2101 atgctaaata aagatggagt gcttactatt atgacaaatg gtaaagtgta cgtaccagag
     2161 tatgaatatg atatggtaaa acaagtttgt gaaacgagaa cactgattga aacatatgct
     2221 ttagaaaaaa tgatgcaaga agaggaagag gtttttcaac aaaagctaga tgtaataaaa
     2281 agatactctg ataaatgcga ggagtactat cgtcaagaaa aatctgtaga acttgcacta
     2341 gcagatttgg aattacataa agccattgta gaaggggcaa ataatgagat tttaaatgat
     2401 gtatatgttg gtatacaagg aagatttata gtagtgaact atttaattcg accattaaaa
     2461 aatcgtaatt atgaaggaac agtacaagac cattttgaaa tattaaaatt cattgaagac
     2521 agagatacaa aaaaagcagt agataaacta agaaatcata ttcaaggaac tattaaaaga
     2581 ttctgtgaag atgaataata agttttgaag ttactatata cttatctatt aagttgctaa
     2641 atgtagtcta agtataatct aattatagtt taaacataat ctaaatataa tctaaatata
     2701 gtgtaaataa gtctaagtat gaattaaata tagattaagt atgaattaag catggattaa
     2761 atatacttta aattacaaat attacaagga agccagaagg cttcctaaaa aaataacctt
     2821 tataaaattt tttattttaa atttaaaatt ttttatttta aatttaaaat aaaaaatgaa
     2881 agaggtggta gagagtttga aaaaaattac taaagaaagg atagaaaata ctatgaaaaa
     2941 gctatttacg agcgaaacat ttttggaata tcaatttata agtgattgcc aaatatcacc
     3001 agatggagga tatacagcat ttattgtaaa aaaagctgat atcaaagaga atggttacac
     3061 aagccaagtt tatatattaa acaataaaac tggcgaatta aaacaaataa cgtctataaa
     3121 ctctgttgga gcatatgtct gggaagatga aaatactatc ttgttccctg ctttgcgaaa
     3181 tgaaaaagta aaagaagctg tcaaaaacgg taagcaatgc atgagttact atgctttatc
     3241 tttaaatggt ggagaagcag aagaactttt tagactccct atcaaaggtg gtaaattgaa
     3301 tcctatagga aaaggtttgt atgcagttat tgattcttat gacaatgacc gacctattgt
     3361 agagggattg ccagaagaag aacaacagaa aaaaataaat gcttataaca aaagacatta
     3421 tgaacatttc aaagaaattc cttatgctgt caatggggag ggatatatta gcagaaaaag
     3481 aaaacgccta tatatatacg acagtaatat aaataagttg aaggcaatta cagcacctat
     3541 gtttaatgtt gtaggtatga aaataagtga tggaaaaatc ttatatatag ggcaagagtt
     3601 taaagatgta aaaggtttga aaaatggggt ctatgtattt gatacaaaca caaacacaaa
     3661 tatctgtata ttagaaaaag ataagtatat tataaaaggt tttgaattat atcaaaatca
     3721 agtgatacta aacctaactg atgctttatc ttatggaaat ggagaaaatg gagattttta
     3781 tactattgat attgatacga aagaaatgaa acttctttca gggcatcaac atcattgtat
     3841 tggaaataca gtaacatctg atgttaaaat gggtgctggt cagacaacaa aggtagatgg
     3901 agaatatatt tattacactt ctacagtaga tatggactgc attattgaaa gaatacatat
     3961 tccaacagga aaacaggaaa aagtaacaca gacaggttca gttgatttta tagatgtaaa
     4021 agatggcaat attgtatgtg tgggttgttt aggaaatgga ttaccagaag tttatacagt
     4081 agagaatgga acattgtgta agaaaacaca tttaaatgac catattttag aggaatataa
     4141 aatttctgtg ccagaataca tagagtcaaa aggaagttct aaatgggaaa tacaaggata
     4201 cgtatataaa ccagtagatt atgaggtggg gaaaaaatat ccagctgtat tggcgattca
     4261 tggaggacca agattgacat atggaccata ttttatgcat gagatacagg tatttacaag
     4321 tgcaggttac tttgtattct tctgtaaccc aagggggtct gaaggtcgtg gcaatgcttt
     4381 tgcagatatt aggaaacagt ttggagatat tgattatata gattttatgg aatttacaga
     4441 tactgtactt gaaaaatata cagacattga taaaacaaaa ttggctgttg aaggtggaag
     4501 ttatggtgga tttatgacga attggataat tggtcataca aaccgttttg ctgttgcttg
     4561 tgctcagcgt tctattgcaa actggagtag catggagggt acaacagaca ttggttatta
     4621 tttctgcaaa ggacaaacag gtgcttctca tatggaaaac cacgagcttc aatggaaaca
     4681 gtcacctctt gcttatgctg ataaatgtgt tacaccgact ttatttttac atggtgaaaa
     4741 agactataga tgctatatgc aagaagcatt ccagatgttt tcagcattga aaattcatgg
     4801 gtgtcctaca aaactttgtc tatttgaagg ggaaaaccat gaactgtctc gttctggtag
     4861 accaaaacaa aagctacaaa gattagttga aatgctagat tggttttctg tgtacataaa
     4921 aaaagataac ataggacagt agaaaggaga atataaaaat ggaaaaaata aagattgaaa
     4981 gttcagatat ttttgaatat atattccctc atgatatttc ttgttcacct gatggaaagc
     5041 atattgctta tattatatca aatatcaacg aggaaaaaga ctgctatgaa catgacttat
     5101 atgtgatgga catcaaaaca gaaaaacaaa tacatatgac tcaaacaaaa gatgtaacat
     5161 catttagttg gatttctaat acagaactac tttttacatc taaaagaaat aagccaaaag
     5221 caggtacaac agatttttac actatttcta tagaaggagg agaagcaaaa gaagcatttt
     5281 ctattccaaa agcttgtagc gtacctgtat cacttggaaa taaattgtgg ttattgacaa
     5341 caaaaaatcc aacggattct aaaaaatcag aaccagatag ggcagtagag ggtgtagatt
     5401 attggacatt tacagataaa ccttttataa gagatagaga aaatttttct caaagaagac
     5461 gtgtaacatt agaattatat caagaaggtg aaaatataac aaaagccatt acaccaaaat
     5521 tttgtgaagt agcaggtata gacgtttctt ctgataaaaa cagaatttta tatactggtc
     5581 agatttatga agattgtgca actccttttt ctggactttg ggagtatcat atagacagtg
     5641 gtgaaaccaa agaacttgta ccacaaggaa agtaccaaat aagtttggca aaatatattg
     5701 gcaaagataa agtaatgttg caagcttcaa cattagaacg ttctatcaca cagaatcatg
     5761 atatttttat acttgatttg agtactagtg aaataaatat gattgctagt ccagatggta
     5821 tgtatgctac attactggat gtagatgcag tatatggtgg tggacgtagt aataaagtta
     5881 taggagataa atttattggt gcaagaattt gcagaactat gacagaattt aatgagtttg
     5941 atacaaagac aggtaatata cgtatcatca caaaagtaga tgcttttaca tcttttgata
     6001 tttacgacaa caccatgtac acagtaatgt tgaaagatta tgagttagca gaaatatata
     6061 gcattgatat gactacaggt acaatgaaaa aaatgactgc tttttctaaa ccatatttag
     6121 atactcataa ggtttcatta cctgaaaaat tgacgtttgt agcaaaaaat aaagaagaag
     6181 ttgatggttt tgttatacca cctatagatg ctaaagaagg cgaaaaatat cctgctgtat
     6241 tgttcattca tggtggacct aaatgggcat atggctatat gtttacacac ttgaagcagt
     6301 gtgtaacatc aaagggaatg tatgtgattt attgtaaccc tcatggtggt gacggttatg
     6361 gtgaaaaatt cttagaaatg gtagaaagat gggggtatgt tgactatgag catcttatgg
     6421 aatttgtaga tacttgtatt gaaaaatatc ctgggattga tgcagaccgc ttaggggttg
     6481 cagggggaag ttacggtggt tatatgacaa actggataat tggccataca gaccgtttca
     6541 aagcagcagt ttctcaaaga ggtattagta acttgattac agcttcttta attattgact
     6601 ttggggatag aattatgaag cagacttgtg gagataagac gccttggaat catgaggagg
     6661 ttctttggaa tcattcacct attaaatatg tgaaaaatgt taagacacca acattgttct
     6721 tacatagtga tagggattat agatgtttta tgggggatac tttccaaatg tttacagcat
     6781 taaaacagct tggtgtagat acagaaatgt atttgttcca tggagataca catggattgt
     6841 ctagaaatgg aagaccatct aatagaattg cacgagcaaa tgctattgta gattggtttg
     6901 aaagatattt ataataaggg ggttattcta tgagtgatat gttattatgg tttagtgtcg
     6961 tattagtttt atttgctatt ggggatttaa ttgcaagcaa aacaaaagca aaagtatcag
     7021 ctgtatttgt tacactactt ttatttttga ttctatttgt cacaaaagca ataccagctg
     7081 atattattga aaaagcaggt atgaccgcag cagctagttg gagtgttcca atgattatgt
     7141 ttagtatggg aacaatgttg aatgttaaac aatttataga cgaatggaga acagtattga
     7201 cagcatggct tggaattgta gcagttatag tctgtgtctc attatgtatt cctttgtttg
     7261 gaaaatcaac agtattgaca agcataccag ttataaatgg agctttacct gctactacta
     7321 ttatgacaca agcagcacta gaaaaaggac tgacacttgc agcagctaca gcaacagttg
     7381 tatttgcaat tcagaagttt attggtacac caatagcttc aagagcagcg ttacaagagg
     7441 caaaccgtct tttagtagaa tatcatgaag caaaatcaaa aggcattgat ttagcaaatg
     7501 ttgatactga taagaaagaa gcagagaata caggcacaaa agtaaaacaa gctttctgtg
     7561 aaaaatacga taaatattat tctacaaatg tttgtatatt ttttatagca ctattctctt
     7621 atctagggta tgaactttca gagattattc atgtaaatta ttctattgta tgtcttgttg
     7681 ttggtgttat tgtaacacgt attggtattg taccaaaaga cattttggaa aaaggaaaaa
     7741 tcaagggatt tattaacatg gttgtgtttg cagctgttat tccttcttta gcaaaagttt
     7801 ctttgacaga tttaattagt ctttttgtac caattgtggg tatgtttgca gcttctatca
     7861 ttggtatatt ccttatgatg aaggtgttac caggttggaa aattatcggt tctaaacctc
     7921 ttgcttttgg agtagggttt tgtcagatgt tgggattccc aactacatat ttaatttcaa
     7981 atgaagtgtg taatgctgtt ggagaaacgg aagaagaaag agcatatttg atgtctaaga
     8041 ttatgcctaa gctggtagtt ggtggtatgg cttgtatgat ttctattgta gtagctggtc
     8101 ttatggtacc aatgttgtag ataggtttca tagttaaaaa gtggtgttaa tttataggag
     8161 aagctaaaaa gtagtattaa tttataaaat aattttatat tagttaattt gttataagaa
     8221 tattttcttt ttatgattat aattttgatt ttataagaga gcagattttt tatttgctat
     8281 gctaatagtt ataatttgtt tttatttgct aaaatgcaac tttaatttga tttatgtata
     8341 atatagggta tttaataata tttttgaaga atttttataa aaaattaaaa agaggagaaa
     8401 aaagggagtg ccacaaagtg aactttttag ttctaaggca ctcttttttg tacgaaatcg
     8461 aatatatttt caacgtttta aaagtgagaa agagacttat ctctagtttg ggatagccaa
     8521 atttaattat taaaattatt ttttgtttta tttatcaaag agtattcgtg ttttagaagt
     8581 tagtattttt tattttatat atccaaggtt ttccgtttct aatgttttct tttttaaaat
     8641 aattagggta ttcaattata gtaaaaggtg gatggtattt tggaatgata ttatttaaag
     8701 cacagtattg aatgttgaaa tcctcacatt ttagagtatt atcttttgta atcaaaaaaa
     8761 tcttctatac ttatagcaag gttacttctg caaatattcc atgcatcata atatccaaat
     8821 ttaaattttt ttaatataga gtttacctct agtctacact cttgaataac tctttcctct
     8881 atccaaattc ttataaactc atcccattta ttactatcta tttattacta tctaaatttt
     8941 ttagtatgtt tgtaattgga aatggattta ttcctttatg ttttttataa atagtttata
     9001 acttaattta ttattaaaca aatcattcat acagaattac tttttctttt agatattcaa
     9061 ttcttacttt taataaatat ttaatacatt ctatcttatg tggttttaaa aagtgtttat
     9121 ggctatcaat aatctctaat atttcattca tatttatttt gtcatagtaa tattttacat
     9181 cttttatatt gacaaaaaag tgctctaagt gtatagtgtg tatataataa catcacactg
     9241 gaggtgataa aaaatgaaag tgctcatttc taacacatca gataagccat tataccaaca
     9301 aattaaagag caaattaaag aagcaatatt tactgaagaa ctgaaagaag gggatgcaat
     9361 gccctcaata agaaattttg ctaatgattt aaaagttagt attttgacta ttagacgagt
     9421 gtatgaagaa cttgaaaatg aaggttttct tacacgccaa gttggaatag gaacatttgt
     9481 ttcaactgga aatttagaaa tactaagaga ctcaaagagg cgtatagttg aacaaaaaat
     9541 ggcagatatg attaaggatg caaaagcact aggaattaca cagatagagt tacaagaaat
     9601 gatgggtatt ctatatgagg aggattaata tggaaaatat tttagaagtt tataacttaa
     9661 ataagactta taccaatttt tcattatcta atataagctt tgtattacca gaagattgta
     9721 taactggatt tataggtgtt aatggagcag gaaaaacaac aacaattcgt tcgatattgg
     9781 atgtcgtaaa gcgtgatagt ggaaaaataa aaatctttgg taaagatttc gatggaaatg
     9841 agaaggaatt aaaaaatcga attggagttg tatttgatga ttcatatttc tatgaggagt
     9901 tgtcaatgtc agaaatgaaa agtattattg caccttcata tgataattgg tgtgaagaag
     9961 attatcaaca aaatatgaag cgttttaatc ttaatccaaa acaaaagatt gatacacttt
    10021 caaaaggaat gaaaatgaaa tttgctttag cattagcatt atcgcataaa gctgagtttt
    10081 taattatgga tgagcctaca agtggtttag acccactaat ccgtagtcag atacttgaca
    10141 tactaacaga atatatgaag aaatgtggtc gtggtgtttt cttttctact catataacat
    10201 ctgatttaga caaaatagca gatatgattg ttatgattga caatggacgt attatattag
    10261 agcgtgaaaa agatactcta ttggatgaac atagaaaagt aaaaggggat attcaatttc
    10321 taaatgatga aatcaaaaaa ttatttttaa atttagaaat tacaggttat ggttttatag
    10381 ggattacctc aaaaattaat gaagtgcaaa aaaatatgcc agatatttta atagagcgtc
    10441 ctacaattga agatattatg cttgcatata ttgaaaggag agagtaatta tgttgattca
    10501 tttagttaaa aaagatattc tgattgtaaa aaaatatgta ttgttgatat tgtttattgc
    10561 aataggaatc ccattattta tattatggcg tttacctcca tttgcacaaa tattgggatt
    10621 tgtactatca attatatttt cagaatttat gctgtgccag tatctattat taaaggagag
    10681 tcaatattca aaagcatctg tattactttg ttcaactcct tatcctagac gtggcttagt
    10741 tcaatctaag tatgttttat ttgcaatggt atttatctat tgtacattag tttactggat
    10801 tgaaaattta cttattccac aaataggaac ttttgactta acatatattt tgattgtttt
    10861 attaataagt tctattattt atggagttta tatgcctatt caatacaaat taggatatga
    10921 aacaacaaaa ttctttttcg tgatgataat catggcttca tcatttgtat taccattaac
    10981 tatagctcac agcaattttg aaattccatt tatatatcag tatccatcca tgatagtgaa
    11041 tattgtattg ttccttttaa gtttaattat actgttagct tcaatgttta tttctattaa
    11101 aatttttagt aataaggagc ttgtataagg taagggggaa ttttatatgc cacatttgcc
    11161 agattgggct gttttacttt gtatcttact tccaatagta tataaagctt gtcagatatt
    11221 taaaagtaaa aaatagagta catatttata ttgccatttt tattgagtga agttagatgg
    11281 tatgaattta aaaggttact tgaaaataaa gttacttggt gtgggagagt aacatcataa
    11341 tattatagat tatatgtttc ttgtgaatca atttgtattt tttaatagat aatatccagt
    11401 aattttattt tccattgatt attttcaatt atgagtatga ttgtgtgtat tgtataccat
    11461 cttttaaggt gtttttaaaa aattatatcg tcaaaatata aatctaagga atcctataaa
    11521 taaagctttt caattttaga aaactaataa aaagtaggaa gttaataaaa agaagctgtc
    11581 tcaaaataga gataagcttc tttttcattg ctgaaatgac gaaaatataa ttgatttaat
    11641 ataaaaaaga gtgctttatg aactatttat tttgaacagt agatacactt gcactcaatt
    11701 tagttactcc taagctttat gaactaaaag tttattttga agcctatttt tctaaataat
    11761 catctatata cagtattgcg tttcttttat ttattcataa tttattttct tattaaagtt
    11821 tttctatatt actttacttt taattttata cgaattatca aaaataaaat agttattaga
    11881 aaaaaataaa agtaattgca aagattgcaa ttacttactt ctatattaat tactatacca
    11941 taaaataaca aaaaaatcaa gtcgagtaat ccttcaactt aagtgatata ctaggtatta
    12001 ataattttta attaataaaa tataactaga ggaaaatcta cattttctca atttctttaa
    12061 ttaaattgac ctctaattaa gtaattataa ggagaaaaat tatgaattta gcaaagggat
    12121 tagatgtttt aaaaatttca tcaaatgtat tcggtgaaga taaagttatg tatattcctg
    12181 taatctacac agaagatgat gcaacactta ttgacacagg tcttccgggt caaggtgatt
    12241 taataattga tgctcttaat aaaagtaaca cgagctttga taggttaaaa aatataataa
    12301 ttactcatca tgatatagac catataggaa acataaatta tctaagagaa aaatctaaga
    12361 ataatatcaa agtatatgca tacaaaagtg aagtcagtta tataacagga gaagaaactc
    12421 cttttaaact ttatatgttg gaacaaatgg tagataaaat tgatgataag atgcttagta
    12481 tgctaaatgt tatggtactt ggatttaaat catcatatac taaagtagat gtgtcattag
    12541 ataaccatga aaaattgaat ctaggtgagg aaatagaagt tatacataca ggaggtcaca
    12601 ctagaggtca catatgtctt tatttaaaag agtctaaagt acttattgct ggagatttgt
    12661 tacaggtaga aaatggagaa cttacgcctg ttgatgtaat gcacagcaac aaacaagagt
    12721 taaaagatgc tattaaaaat ataagtaatt atgatataga aacgatagtt tttagtcatg
    12781 gtggattata tcaaagagac ataattggaa cattgaaaaa cctaattatt gagtagtact
    12841 atatgattta caataatttc ttaaattact taaaattata tctaaagatg tagaatcaaa
    12901 attaaatata agtaataaga attttgcttt aaaattatac ttaagtgtta agtatgatac
    12961 gtagtattac aagtatttta gaatataatt ttttatagta attttaaggt tctacaatac
    13021 ttcagttgaa aaatatttaa cagcatagta taataggaaa gaaaatattt taaataaact
    13081 ttagtataca aagtgaagaa aaatatttaa attatatata atattgtaga gtgaggtaaa
    13141 atatttaagt tatatatcat aatacagagt gaagaaaaat atttagataa tgtaccataa
    13201 tacaaggtga gtaaaaatat ttaaagcatg tataataatg caaagcgaag aaaaatattt
    13261 aaataatgta ccataatgca gagtgagtaa aaatatttaa ataatgtgtt ataatacaaa
    13321 gcgaataaga ttatttaaag catgtattat aatataaagt gagtaaaaa
//
LOCUS       DAEPOZ010000106        13346 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.106, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000106 DAEPOZ010000000
VERSION     DAEPOZ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13346
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.106"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            81..2321
                     /locus_tag="KR440_002178"
     CDS             81..2321
                     /locus_tag="KR440_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBH0699226.1"
                     /translation="MSNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNDIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSRDGEISEATKKKLSKLLIEDRCPICDGRRLNEGVYRSLI
                     NGYNIADLTSMQIDELAEVIKSINEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHVVDVGPKAGKYGGKVVYEGSYKNLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEIKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNVCEGKKFKKEVLEYRFQRKNIIEVLEMSVLDAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            2735..4270
                     /locus_tag="KR440_002179"
     CDS             2735..4270
                     /locus_tag="KR440_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBH0699227.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTAMTISGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIATIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGIGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNISDLKNVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVIVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSEVFKMVGDILTDKEMAKMNYDVENNNLEPREVACEFLNKK
                     GLL"
     gene            4286..5047
                     /locus_tag="KR440_002180"
     CDS             4286..5047
                     /locus_tag="KR440_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0699228.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            5385..6253
                     /locus_tag="KR440_002181"
                     /pseudo
     CDS             5385..6253
                     /locus_tag="KR440_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            6447..6698
                     /locus_tag="KR440_002182"
     CDS             6447..6698
                     /locus_tag="KR440_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBH0699229.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            6810..7589
                     /locus_tag="KR440_002183"
     CDS             6810..7589
                     /locus_tag="KR440_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH0699230.1"
                     /translation="MNNTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFIDYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(7724..8374)
                     /locus_tag="KR440_002184"
     CDS             complement(7724..8374)
                     /locus_tag="KR440_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699231.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDSTSK
                     YPDALNAILNCDRLIIAFPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIDHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(8371..8874)
                     /locus_tag="KR440_002185"
     CDS             complement(8371..8874)
                     /locus_tag="KR440_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH0699232.1"
                     /translation="MKLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGRFGCWI
                     KTPSECVVKDYYSNMGKLINESDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(8923..9438)
                     /locus_tag="KR440_002186"
     CDS             complement(8923..9438)
                     /locus_tag="KR440_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBH0699233.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGSKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKIDVEENHCIDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            9754..10245
                     /locus_tag="KR440_002187"
     CDS             9754..10245
                     /locus_tag="KR440_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0699234.1"
                     /translation="MRTIDIKGENMRELLKWVSIINRYNTNYIDRKLADIGINNSQYF
                     YVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGVKDKRTWHLYPTE
                     KSLECYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDMNEEAKLEAKENG
                     RKI"
     gene            10229..11632
                     /locus_tag="KR440_002188"
     CDS             10229..11632
                     /locus_tag="KR440_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0699235.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQFFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
     gene            complement(12574..13167)
                     /locus_tag="KR440_002189"
     CDS             complement(12574..13167)
                     /locus_tag="KR440_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH0699236.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
BASE COUNT         4999 a         1515 c         2249 g         4583 t
ORIGIN      
        1 atttcactta tgtataacta acaactatta ttacgatttt ctctaaataa tttaaagaaa
       61 aataatgcag gaggtaaaat atgagcaatg aatttataat tttaaaaggt tgcaaagaga
      121 ataatttgaa caatatttcc ttaaaaatac caaaaagaaa aataaccata ttcacagggg
      181 tgtcgggttc tggaaagtca tctattgtat ttgagacaat tgcaaaagaa tcacaacgcc
      241 aactaaatga aaggtttagt acatttgtaa gaagtttttt acctaagtat ggagaggtta
      301 aagctgattg tatagaaaat ctgtcaacac caattatcat agaccaaagt agacttggtg
      361 gaaattcaag gtcaacccta ggtactatta cagatgtaaa ttccttttta agagctttat
      421 attcaaggtt tggtagtgag tatataggta atgctaatat gttttctttt aatgatattg
      481 atggtatgtg cccacactgt catgggcttg gaaaaaagct agttcctaat atgaaccaaa
      541 ttcttgatat gaataaatca ttaaatgaag gtgctatatt gctttcaggg tttggagtgg
      601 gttcttggca ttggaatata ttcgctgaat caggattttt tgatattaat aagaggatat
      661 gtgattattc agaagtagaa ttggaaaagt ttttgcatgg taaagcagaa aagattaaga
      721 tagaaaatgc tggtcaaact aacatgacat atgagggatt aatggttaaa tttaatcgtc
      781 tatacttaag tagagatgga gaaatatctg aggctactaa gaaaaaattg agtaaacttc
      841 ttatagaaga tagatgtcct atatgtgatg gaagaagact taatgaagga gtatataggt
      901 ctttaattaa tggttataat atagcagatt taactagtat gcaaattgat gaattggctg
      961 aagttattaa aagtataaat gagccagaag cacaaccttt aattaaagga ataatagaaa
     1021 agttaaacag tatcatagat ataggtcttg gatatttaac actggataga gagacatctt
     1081 ctctatcagg aggggaatct cagcgtataa agatggttaa gtatttaaat agtaatcttg
     1141 ttgatttaat gtatattttt gatgaaccaa gtgtaggact tcatccaaaa gatgtgtata
     1201 aattaaataa tcttttaaaa aaattgcgtg ataaagggaa tacaattata gttgttgaac
     1261 atgacccaga tgttattaag attgcagacc atgttgtaga tgtaggacca aaggcaggta
     1321 agtatggtgg aaaagttgtg tatgaaggca gttataaaaa tctacttaca agtggtactt
     1381 tgacagggaa tgctttaagt aaatttcttc ctattaaaga aagtgtaaga gaacatagtg
     1441 gatatttaga aattaaaaat tgtaataaaa acaatttaaa aaatgtatct attaagatac
     1501 ctaaaggtgt attgactcta gttactggag tagcaggagc aggtaaaagt acgttgatta
     1561 aggatgaatt tttaaagcaa aatcctagtg ctgtacttat agaccaaagc cctgtttcag
     1621 caaattcaag gtcgagtttg gcaacatata gtggaattat gaacaacata agaagcatat
     1681 tttcaaaaac aaatggcgta aatgcttctt tattcagttc aaattcagaa ggagcttgtg
     1741 ataactgtaa gggttctgga atagtagaaa tgaatcttgc ttttatggag agtataaaga
     1801 gtacctgcaa tgtttgtgaa ggtaaaaaat ttaaaaaaga agttttagaa tataggtttc
     1861 aaaggaagaa tataattgaa gttcttgaaa tgtctgtttt agacgcaatt gagtttttta
     1921 atttaaaaca gattaaaacg aaattacaat gtattgaaaa aatgggtatt ggatacctta
     1981 ctttaggaca aactcttgat actttgtctg gaggagagtg tcaaagatta aagcttgcta
     2041 gtgaattaaa taaggaaagt tcagtgtata tattagatga accaacaaca ggtcttcata
     2101 tggctgatat tgagaatttt ataagtataa ttgaagagat tgtagataat ggaaatacgg
     2161 tcataataat tgaacacaat atagatgtaa ttaaaagagc tgattggatt attgagctgg
     2221 gacctgaagg agggacaaaa ggcgggagag taatatttga aggtatccca aaacaattat
     2281 gtaacaataa aatatcatta acagcaaaat atatagtata ataaataaaa tataataaaa
     2341 ttataatgat attttaataa atataatggg tatataaaat gataattaaa cttaacagta
     2401 ataaataatg tgagaaatat tcaaatttaa ttaaataaag tgtcagaaat ttgtttaaat
     2461 tttagtattt taacgattct gacctttttt cttattttta tggagagtat cgaagatgga
     2521 gtctctaata gttacttttt tgtgtctact tgcaagtttg attaaaaaat ggctatacac
     2581 acttgtgact tcagtcatgg gaggttcaga ttataatctt aatatataat ttgtgaaata
     2641 atttgatgac gatttgctga tgtataaaca ataaagatta actacattaa aatttaaatc
     2701 ttgttaagta aaacttataa aaggagagag atggatgata aatgatattc taaccttatt
     2761 atctgaaaga agtgattttt ttataaagct atccatagaa catttgatga tttctattat
     2821 ttctataata attgcgattg ttttgggtgg tataattggt attattgtaa gtgaatttca
     2881 taattcttct aaacctatat tgggaactat caattttcta tacaccattc catctatttc
     2941 tatgctagga tttctgatac cattttccgg aattggaaat actactgctg ttattgcact
     3001 taccatttat gcattattgc caatggttag aaatacatat actggaatta ctaatgtgga
     3061 taaaaatatt cttgaagcag ctaggggaat gggcagtaca gattttcaga tattgtataa
     3121 aataaaactt ccattagccc tacctgttat tatgtctggt attagaaata tggtgattat
     3181 gacaattgca ttagctggta tcgcttcatt tataggtgct ggaggcctag gtgtagccat
     3241 atatagagga atcacgacaa ataacacagc aatgacaatt agtggaagta tacttattgc
     3301 tctattagct ttaatatttg attttatttt agggctggta gaaaaatttt ttcaacgaaa
     3361 aagaaaaaag aaaaatagga aaaaagtttt tgccattcta ggaagcctta ttcttatagc
     3421 aactatcttt acgtcttatt tatttaatcc ttcaaagact caaactattc atattgctac
     3481 taaacctatg actgaacaat atattcttgg tgagatgttg gatattttaa ttgaaaaaaa
     3541 cacaggtcta aacgtggaaa ttacacaagg cataggaggg ggaacatcta atatccagcc
     3601 agctatggag aatggagaat ttgacattta tccagagtat acaggtacag gctggaatat
     3661 ggtacttaag aaaaatgaat tatatactga aagtatgttt agtaaactac agtcggaata
     3721 caaaactaaa cttaaaatgc aatggactgg tatgtatggt ttttctaata gctatgcatt
     3781 agcagttaga aaagaagttg cagataaata taatttaaaa aatatatcag acttaaaaaa
     3841 tgtatcttct aaattgacat ttggagctga atatgacttt tatgagagag aggatggata
     3901 tactccattg tgtaatacat atggactgaa ctttaaagat acagttgatt tagatattgg
     3961 attaaaatac caagctatca atgaaggtaa gattgacgta atgcctatat ttacaactga
     4021 tggtcagtat agtgtttcag atgttatagt tctaaaagat gataaaaatt tctacccatc
     4081 ttatcaatgt ggaaatgttg ttcgtttgga agtattagag gaaaatccag aactatcaga
     4141 ggtatttaag atggttggag atattttgac tgataaagaa atggctaaaa tgaattatga
     4201 cgtagaaaac aacaatttag aacctagaga agttgcttgt gagtttctga ataagaaagg
     4261 tcttttataa attggaggaa atataatgac acctattata caatttaaaa atattaaaaa
     4321 acaatataat gataaaacta taatagataa tcttaattta gatatagaaa aaggagagtt
     4381 tttgactgta attggttcgt ctggttctgg taagacaaca ctcttaaaaa tgattaatgg
     4441 tctgattttg ccagatggag gaaatatttt gataaaccaa acagatatca aaaacgagga
     4501 cttaattaaa ttaagaagaa gaataggata ttgtgtacaa ggaagtgttc tttttcctca
     4561 tatgactgtt gaagaaaata tttcatatgt accaaatctc ctaagtaaaa agaataaatt
     4621 agaagtaaaa agtgctgtaa acaagtggat ggaaatagtg ggattgccaa atgatatgaa
     4681 agtgcgttac ccatcagaat tgtcaggtgg tcagcaacaa cgtgtaggga ttgcaagagc
     4741 tttagcttct tctccagaaa ttttattaat ggatgaacct tttggtgcag ttgatgagat
     4801 tacacgaaag caattacaaa aagagataaa agaaattcat aaaaaaacag gagttacgat
     4861 tatctttatt acgcatgata tttatgaagc tttgattttg ggaacaaaga ctttggtatt
     4921 aaatcatgga gttgttcaac agtatgatac tcctgagaat attttaaata ctcctgctaa
     4981 ccaatttgta gaccagttat tgaatataag aaaatctata atagatgatg ataatttaaa
     5041 agaataaatt taaatttaaa atcaaattgt tgtgataaaa actattgatt tagagctaac
     5101 ttcaagttgt atacttgaag tggaaaagtt agattaaggt atagaagaga atttagcagt
     5161 tggctgtaga cgaggtaact gctgaaggtg ctttcaataa atggctagaa cctgtatcta
     5221 ttgaagatta taataaattg aaataagaag ctcaatatac agagtagata ggcacttcaa
     5281 atagaagtgt cttttgtgtg ccataaaata aatcaaaata ttagcaaaga atgtgaaaaa
     5341 caaaataaaa taatatgcta tctttaaaat aggaggggtg ctatatggat aacaatttga
     5401 ttaccattga aaaaacttta gattatatag aaaaacatct tttagatgag atgttagatt
     5461 taaatgtaat ttctaaagag ataggctatt caaagtacca tttacataga atgtttacat
     5521 cagttgtcgg atttagtata cataattata tacaaagacg tagactaact gaagcagcaa
     5581 gaatgctcat ttttacagaa aaaccaatta ttgaaattgc tttaatctcg gggtatgaca
     5641 cacaacgttc tttttcaaca gggtttaaaa aaatgtttaa gcatagccct aagtttttta
     5701 gaaaacatca agatttttta ccactacaat taaaatttga tattaataat agaaaaaggt
     5761 tacatggagg tatggtttta gatgttaaaa aagttaattg tacagatata aatattgttg
     5821 gctattctgg taaagtaaaa aaaggatttc atgttattgg aaaatgttgg agggatttgc
     5881 ataaaaataa agagttgata gaaaacaagg tagacttgga ttttttaatt ggagttaatg
     5941 attatagtaa ttttaaagaa attgaaaata atcctacatt tacatatatt gcaggtgttc
     6001 aagtagctac ttttggtaaa ttcctaatgg aatggaaaaa ttcgtattac cgaaaagtaa
     6061 ttatattgtt tttactttta ttgggaaaaa tgaggatagt atgcaaaatg ttgtagaata
     6121 tatctatcaa gaatggttcc ttaattcaag ttgccagttt aatgaaaata atttatatga
     6181 ttttgtaaaa tacggagaaa aaactgataa gaatggtttg agtgaaattc agttttgggt
     6241 tccaatcatt taaaagaagg gattatgtat aaagtaggtg gatttatata ggcaaagttt
     6301 tataaaagta tgtatttata aataatttat agtctgtagg aaagaaatag ttttactata
     6361 agattatgat aaaatacaca atactatgat aaaatagtac taaataatag atttaatttt
     6421 gcaaaggata aagtgtttta ataaaaatga atgtagaaaa cgaattactt aaaaacctag
     6481 atagattgca tacaacagag ttaggcgttg taagaattaa gaaaaatctt tctttggaga
     6541 caaatgatgt agtgaactgg tgtaaaacta aaatagaatc tccaaatgcc attattaata
     6601 gaaagggaaa aaactggtat ataagtgtat atgattgtat tataacagta aacgctcaca
     6661 gttatacaat tattacagct cataaagaaa agaaatgaat tgaaaaatac aaaggtcaaa
     6721 agcattttcc actgtttgta gtacctatga agaagtatat aaaataatat tacttttgaa
     6781 taataattta aattttggag gttgaaaaaa tgaataatac tgtaaaagaa aaattggcag
     6841 agtcactaac ttctgatact actgatattt taccttattt gccttattta ttacaagatt
     6901 tttgggaatt aggaagttca ccagaacaaa tgttacaatt atttagaaag catatacctt
     6961 ttaatgagga tacaaaagtt ttggatttag cttgtggaaa aggtgctgta tctgtgaagc
     7021 ttgcaaaaga gttgaatata catatcaaag gtattgattt gatacctgaa tttattgatt
     7081 atgcgagaag aaaggctatt gaatataagg ttgatgcttt gtgtgatttt gaaatgaatg
     7141 atgctaatag ttctgtaaaa attgagcacg attatgattg tgtgattttt ggagctgtag
     7201 gggatatttt aggtaattat aaagaaacta taagtaagct attacctaca ataaaaagta
     7261 aggggttcat tttaattgat gatgcatatg tagtagataa atctacaaat gaacagctta
     7321 attttgagag ggtgtatccc acttacaatg agtggtgtat attgttcaaa gaattaggtc
     7381 tgaaactaat tgaatgtata ccatgtttag aacttgaaaa tgatgttgaa gaggaaatga
     7441 actggattat aactcgtgca aatgagctaa ttaaaaaata tccttctcaa aaagatatgt
     7501 ttgaacaata tgttattagc caaaaaaatg aatatactga tttacagcag gaccttgttg
     7561 gagcaacttg gattttacaa aaagaataag ataaatatta gactagataa tcaatgttgt
     7621 agtgaattat cccatgtcta gtgaatataa ataacataaa aaaccgtcaa gatgaatagt
     7681 ttattcgtgc ttgacggttt ttagtatttg ttgaagtaaa attttatttc tttagtatat
     7741 cacttttagt caatccattt gatttagctt gagtattcca ttgatgattc cccataagtt
     7801 tatataggaa tctagggaag ttaggtgaaa taaaaatagt acttttgttg gaatcatcta
     7861 tatgtatatt atcactaagt atattttttg aaagttcttt taatgcaatt cctaaatctt
     7921 ttttaggtcc tttacccaaa ggtacggatt ctagtgaacc aaacatctcc ccagctccaa
     7981 ttccaatacc ttgaacccat gtaaagttga tttttttgca ccaatgatct atcatttgaa
     8041 ttgccaaatg attatgttca ccttcattga atccacagtt aactattgca tacactggaa
     8101 taggtttttt attttgtgtt tttaaaaatt tttcacattt ctctaaaaaa tgaagtaaat
     8161 gagatggaat accatctaca taaagaggaa atgctattat taatctatca caattaagta
     8221 tggcattaag agcgtcagga tacttagatg tgctatctac tttaaatgta aaaactttat
     8281 tatcattttg aataagttgt tttaattcag ataaaaaata ctgtgaatta tttttattta
     8341 cttttggact accatttatc attactatat tcatatggat tcacctctta tatcctgtat
     8401 attttcatag aaacaaacag agtgttcttt tatattgaaa tttatagcat ttccatttac
     8461 taatttcgta gcagtttctt tttcagcttc tgtaatacaa tctccataga atatagcttt
     8521 taaatttatc tgtttatcat atctgctttt atgacgcatc atattatctt ttattacaaa
     8581 gtatggaaga atataattaa tgcttttatc aagtacgttt ttgacaaaag ggctaaaccc
     8641 tccgtaacag catctgctta ttataataat tccatcggat tcatttataa gtttacccat
     8701 gttgctgtaa taatctttta caacacattc agaaggagtt ttaatccagc aaccaaaacg
     8761 accaatacat ttgtggattt cttttgtgtt tggaagtatt tttatattgt catcaagctt
     8821 gggtaataac ttttctaatt ctttgtcagt taaatcatgt attaaaagct tcataaagtc
     8881 catctccttt tattatgtat gtttatttct aaaaattata tcttatagat ttttcagtaa
     8941 atctttacac caattaataa caaaattata atagtcaact ccataaatca ttgtggcata
     9001 catatatgta tattggtcaa tacaatgatt ttcttctaca tcaatttttt gtttacttaa
     9061 tttatttaga agtaattcta cttcagttat taacattttt aagtttgttt caattatttc
     9121 tttgggtaga tacttgtaaa agaatatatt aacaaggtgg tcttgctttg ttttagaaaa
     9181 atttataggt ttctttaacc attcgagaaa tacttcttta ccagtattag ttatggaata
     9241 tagttttttt aatttgctac catctataac ttcgtgatag tgtatatatc cttttgtttc
     9301 taatctttta agtgcaggat agatacttcc aaagctggcg tcaaaaaagt acgaggtact
     9361 ttctgacatt atttgtttta agtcgtatcc acttaattct ttttccatta aaaatcctaa
     9421 aataatgtat tctaacatat tacctcctgg tatcaatcag atatatatcg attcgatata
     9481 tttattatta cagataataa atattacgtc aagatgcttt ttaaaataag ctaaaaagaa
     9541 ttaggaatta ttgataaata tgcaaaaaaa ttaatataaa aggtaattaa atatcaatga
     9601 agatttaaaa atagttaaat taaagtaaga gtctaatata taaaatttat agtaacttta
     9661 tggtttaaat ataaacttgt gtgctattga caaagtataa aattagcagt aaaataattg
     9721 catgagcaaa tagattattg cgaacgcaaa aaaatgagga caatagatat taaaggagag
     9781 aatatgagag agctattaaa atgggtttcc attataaata gatacaatac aaattatatt
     9841 gacaggaaat tggcggatat tggaatcaat aatagccaat atttttatgt attacacata
     9901 tgtaataatc caggtattac acaggacact atatttgaga aaattcttgt gaatcctagt
     9961 aatataacga gagctttggc aaatttagaa aaagaaggct ttataacaag agaaccaggt
    10021 gtaaaggata aaagaacatg gcatttatat cctacagaaa aatctttaga atgttatgat
    10081 gaaatactaa aaataacaaa tgggtatgtt gaggagttgt tgagttcatt tgagaaagaa
    10141 gagcaagagt tgtttatgtc aatgttaaag aaagttgcat ttttagctat tgatatgaat
    10201 gaagaagcaa aattggaggc gaaggaaaat ggaagaaaaa tataaggagc cattggaata
    10261 tgaaaaggtt tcaaagttat taactaagta cgcaattcct agtattatag caatgttagt
    10321 aagtgcttta tataacattg ttgaccaaat atttattggg caaggtgttg gtgtattggg
    10381 taatgctgct acaaatgtca cttttccact aacaacaata tgtactgcaa ttgcattatt
    10441 attaggaatt ggaggagcgt ctaagtgttc attggaatta ggtgctaaaa attcagaaaa
    10501 agctacaaaa gcagcaggaa attctatatg cttgatggct gtttttggaa taagtttatt
    10561 tatagtagta tcaatatttt taacaccact attaaaattc tttggctcta catcagaaat
    10621 actaccttat gcacacacat atacaaaaat tacatcaatt ggattaccat ttcttataat
    10681 gtccactgga atgagtaaat tgatacttgc agatgggaga ccaaaagctt ctatgttttg
    10741 tatgttaata ggagcagtta ttaatacaat tttgaatcca ttattcattt ttgtttttaa
    10801 tatgggaatt gctgggtctg ctttagcaac tgttattggg caacttatat cttttggaat
    10861 aagtgttcat tatataaaga attttcaaca tattaaatta cataagaaaa gttttaaatt
    10921 agatagtata atttataaac atatatttgc gctaggttca agctcatgtt ttaatcaatt
    10981 agcaatgact gtaacacaga tagttatgaa taatacattg gcatattatg gggctaggtc
    11041 agtttatgga agtgagattc cacttgcttg tgtcggtatc attattaaag taaatatgat
    11101 ttttatatct ataattattg gtatatcaca aggtatgcaa ccaattatag ggtataatta
    11161 tggggctgaa aagtataatc gtgtaaaaga agcatataag tttgcagtgg gaagtgcaac
    11221 tattatatct gttattgcat tcttatgttt ccaattcttc ccaagacaaa ttacgtctat
    11281 atttggtaac ggaaatgaga cttattttat atttgcagaa cattatttta gaactttctt
    11341 gttcttaaca tttataaatg gtgttcaaat agtatcatca actttcttta catcaattgg
    11401 gaaatcagtg ttgggacttt ttacttcttt gactcgtcaa attttatttt taattccatt
    11461 gatagtaatt ttaccaatgt ttatgggaat tgatggagta atgtttgcag gaccaatagc
    11521 agatgcagca gcggcaatgg tatgtatata ctttgcagtt catgaaatga aatccttgac
    11581 tataaagcaa aataatttga tgagtaacca taataagatt gcagaggtat aagacaatat
    11641 ttaagaagta tagaaaatta atttaagaaa tataataaac taatttagga agtgtagaaa
    11701 attaatttaa agaagtataa taaactaatt taggaagtgt agaaaattaa tttaaagaag
    11761 tataataaac taatttagga agtatagaaa attaatctaa agaagtgtaa taaactaatt
    11821 taggaagtat agaaaattaa tttaaagaag tataatgaac taatttagga agtatagaaa
    11881 attaatttaa agaagtatag aaaattaatc taaagaagtg taataaacta atttaggaag
    11941 tatagaaagc taatctaaag aagtataata aactaattta ggaagtatag aaaattaatc
    12001 taaagaagta taataaacta atttaagaaa tatagaaaat taatttaaag aaatacaata
    12061 cattaatttt taagtaacat taaattaatg taagtaaaat aatgttcata tgaagattta
    12121 aaggtgcatt ttctgaagct aagtatatgt tatgtctcta tatccggtga agtttaatag
    12181 ctcaagaaaa tgcactattt atgatgatta ttttgtttta tttccaattt tggaaataac
    12241 tattctaatt aaatctaaaa ttataccata aataagaaaa gaaagtatag atgctatgta
    12301 ccaataaatc gaaattgacc ctgtaattgt tctacatggg atttgcatgc ctgaatccat
    12361 aaaaacaaaa aaactatcaa tggtagaaat aatagacaaa ataggattta atcctattag
    12421 aataatactt tttgagtctt gaccaatttg gtggatataa atctcaaata atgcaattaa
    12481 aaaaaacaaa atgtaaaaga tttaataatt tttttcatca aaatacctca cctaatataa
    12541 attctgtttg cactatttgc ttactataac tatttatctc cattcagtca catcacttaa
    12601 ttgtaatcta ggtcttcttg atggttcttc gtcaggataa cctaaagata ttgctgccac
    12661 taattgacta tctgtattta accattcaca taattcacga tatgcaaagt atacatcaca
    12721 aatccaaaga ctacctagac ctaattccac agctgtaagc gacatattct gaattgcagc
    12781 tccaattgat tgcatgtttg ccatttcata gaatcgttct tcaggggata gcttttctaa
    12841 aggtgattta cctaatatat ttaaaataaa aattgttact ggagcttgtt tcatgatttc
    12901 aactgtgtaa tttgcaccag cgatatgttg acgacttcct ggaagaagcc cattatcatt
    12961 aatttcattt tgaatacctt tgctcatggc tttaagcata gattcttttt ccttttcagt
    13021 gatgacaaca aaacgccatg gttgcctgtt tttagatgaa ggtgcattta ttccagcttc
    13081 aattattttc tctatagttt catgtgaaat gctttgattt ttatattttc tgatacttcg
    13141 tctttttgat atagaatcac ttatcatttt aagtaacctc cgtttatttt ttggaaaaat
    13201 ctatttattt tctattttta attattttag cattaatcag aaaaaaagtc attgatttta
    13261 gtatacaatt attaacgatt atattaagga ttgtatcaaa aagtatgtga ttatactaag
    13321 ggattatatt agacattata tttaaa
//
LOCUS       DAEPOZ010000107        13230 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.107, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000107 DAEPOZ010000000
VERSION     DAEPOZ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13230
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.107"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..522)
                     /locus_tag="KR440_002190"
     CDS             complement(<1..522)
                     /locus_tag="KR440_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBH0699237.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEITIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNG"
     gene            complement(845..2089)
                     /locus_tag="KR440_002191"
     CDS             complement(845..2089)
                     /locus_tag="KR440_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBH0699238.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGIHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLINKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            2417..3205
                     /locus_tag="KR440_002192"
     CDS             2417..3205
                     /locus_tag="KR440_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBH0699239.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            3205..3678
                     /locus_tag="KR440_002193"
     CDS             3205..3678
                     /locus_tag="KR440_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBH0699240.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(3770..4210)
                     /locus_tag="KR440_002194"
     CDS             complement(3770..4210)
                     /locus_tag="KR440_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HBH0699241.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(4225..4518)
                     /locus_tag="KR440_002195"
     CDS             complement(4225..4518)
                     /locus_tag="KR440_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBH0699242.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            complement(4561..4962)
                     /locus_tag="KR440_002196"
     CDS             complement(4561..4962)
                     /locus_tag="KR440_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699243.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQVGK"
     gene            5195..7315
                     /locus_tag="KR440_002197"
     CDS             5195..7315
                     /locus_tag="KR440_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0699244.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVVDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHIARKILSINANEMIDSFVGDCIKDINFQNILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERAADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLGK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQDFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            7540..8706
                     /locus_tag="KR440_002198"
     CDS             7540..8706
                     /locus_tag="KR440_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH0699245.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESVLVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIK"
     gene            8731..10173
                     /gene="nhaC"
                     /locus_tag="KR440_002199"
     CDS             8731..10173
                     /gene="nhaC"
                     /locus_tag="KR440_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBH0699246.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAATKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDILNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            10176..10445
                     /locus_tag="KR440_002200"
     CDS             10176..10445
                     /locus_tag="KR440_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699247.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMKGISINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(10521..11948)
                     /locus_tag="KR440_002201"
     CDS             complement(10521..11948)
                     /locus_tag="KR440_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HBH0699248.1"
                     /translation="MKLTKSISSMLCLALVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFLNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVKIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEIFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            12319..13188
                     /locus_tag="KR440_002202"
     CDS             12319..13188
                     /locus_tag="KR440_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0699249.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
BASE COUNT         4608 a         1937 c         1804 g         4881 t
ORIGIN      
        1 accattaact ttaatcttag attcatctat tcctttaaaa acaagttctt ccttagtaga
       61 tttactagct actaaataac agtctgtatt tttactaatc cattctgagt gactacttat
      121 atcagttatg catgttatta aaggaagatt tgagttatat ttttccttat atcttgatac
      181 taattgagaa caaaatggaa aagttgaaat aactactgtt ggttgtactt catgaagtaa
      241 tgtatctaat ttatttaaaa aataatctga aaaagtaaat tttatatctt tctttccatt
      301 ttcagcacat ttataaaaca aattataaag actacttccc cgatttacaa gaatagaaaa
      361 agtcttgtac attttatctg aatagttagg cataatgtat tcaaatatat ctttaattgt
      421 tatttctgaa ttatcaaatt ttgcttttat atcttcactt aaagaatttg atgctgagta
      481 atgacccata ccaaattttc ctgtaagaat taataccttc ataagattcc ctcctcgtta
      541 aagcttttct tttcatatga ttactatact tataagtata gactttaaat ctttagaaaa
      601 acctagtata attcttaaga aatcttaaag ttttaattaa aaaatacaaa gagaccatct
      661 aaaaatagag ataaccatat tttttattat ttaaataaca caaatatgtt cgattccata
      721 caaaaaagtg cctcataaac taaaaagttc attttgagac acctttttta tatctaaata
      781 tattcaaata catacacaat cattgtaatt atttaaatgt attttattta gttgttcttt
      841 ataattaatc cataatatta tattcttcat ataaaggata tttttcagtt aaaccaacaa
      901 ccaatgttct agccttagat tcttcatcat aagttaaaca taggtctata gcctcagcga
      961 ttattgccat atcttcttct ttcatacctc tagtagttac agctggagta ccaagtctta
     1021 ttccacttgt aacaagagca ccatttggat caaatggaat tgtattttta ttagctgtta
     1081 tataagcatc atccaatctc ttttctgctg ctttaccagt tatattttta ttaatcaaat
     1141 ctaatagtat taaatgatta tctgtacctc cagaaatcaa tttaaaatct cttttaataa
     1201 gttcttcagc taaggctttt gcatttttag caacttgtac ctgatattct ttaaactcat
     1261 cacttaaagc ttctttaaag caaactgcct tagaagcaat tatatgttca agaggtccac
     1321 cttgaatacc agggaatatt gctttgtcta tgtctttagc atatttttct ttacaaagaa
     1381 taacaccccc acgaggtcct cttaaagttt tgtgagttgt tgttgtaaca aaatcagcca
     1441 cttcacatgg attttggtga ataccagcag ccacaagacc tgctatatga gccatatcta
     1501 ccataagcaa tgctccaacc tcatcagcta tctctctaaa ctttttaaaa tctatttctc
     1561 taggatatgc acttgctcct gcaactatca ttttaggttt tacttcatgt gctatctttc
     1621 taacttcatc aaaatctata agtccactgt ctttatctat accatattca taaaatttat
     1681 aatattgacc agaaatatta actggagcac catgagttaa atgtccacct tgagataaat
     1741 tcattcccat aacaacatca cctggtttta acatagcaaa atatacacca atatttgcat
     1801 ttgctccaga gtgaggttga acattagcat gttctgctcc aaatattttt ttgattctat
     1861 ctattgcaag agtttcaact tcatctatgt attcacatcc accataatat cttttgcctg
     1921 agtaaccttc tgcatactta tttgtaagat gacttcccat agtttccatt actggaactg
     1981 atactatgtt ttcagatgct attaattcaa tatttctttg ttgtcttttt aactctcttt
     2041 tcatactttc ataaatctca ggatccgctt ttttcaagtt ctcaaacata ttactcctcc
     2101 tagttattat ttaaatttta tttttacaga catttcataa ttttcgtttt tatcaatatg
     2161 ataatttatt attatagtcc tttaccaatt aatagtcaag tgttttgcca tttttccata
     2221 tttaacatag ttactttctt tatttttaag ttaaaatata ataaataata ttgtctaatt
     2281 cttttaattg gatataattt aaatgtgact attttgaaac aaaaaaaaga taatatattt
     2341 atatagatat taaggaatat tacttaaaaa tttgatataa tttcaatatt acgtttttta
     2401 atttggaggt gtatttatga atcaaaatga ggaacttgaa tataagaaaa atattctaaa
     2461 actagctcta tttgtagggg aactgatgat aaaaaatgga gctgaaacat ctagggttga
     2521 agacagtgtc cttagaatat gcaggtcaag agggttttat catgtaaata tttttacaac
     2581 tcctacagta ataattattt cagatgagaa atttgatggc ttttcgttta tgaaaacgat
     2641 tcaatcaaga ggtataaact taaacaagat ttctttatta aacagttttt ctcgagaatt
     2701 tgttagtgac aaagagtatg atattgataa tgccattgca cgtctttatg aaatccaaga
     2761 tgtaaaacct tatcctttat ggttatttta ctcttgtaca ggaattgcat ctgcttgttt
     2821 tgcttgtttg cttggaggga attatgtatt aaattttata cttacgttta taatagcaat
     2881 ctttgctgca attacttatg ataaaacgat gaagataagt gcaatatctg cattttcctg
     2941 tttagtatct tcttttttaa ttgcactttc aggagttcta ctagttgaaa taggcatttt
     3001 agatgaccct aaaatgctta tagtcggcgc tataatgcca ctactcccag gagttgcttt
     3061 tataaaagct attagagatt taatatcagg gaatttaata tcaggaattt ctcgtgcttt
     3121 tgatgctgct atgattatta ttgcaattgc ttctggagtt ggttttgtcc ttgatacatg
     3181 gtatagaata ggaggtcctt tctaatgact gatatgtcta tgattttgca ttttttattt
     3241 tcaggtgttg caactgctgg atttgctgtt ttctttaatg ctccactata tctacttctt
     3301 ccagctggag taactggcgg aattggttgg attgtatatg tttatttatt taattttact
     3361 acaaatgcag tttttgcagg ttttattgct gctgctttgg tctcagcgtg tagtgagact
     3421 ttagcaagaa aactaaagca accagctatt gtgttcgtaa ttccaggtat tcttcctcta
     3481 ataccaggaa ttgggctata taatacaatg ttgtctttga ttcagaaaaa ttattctttg
     3541 gctatgtcaa aaggtacaga tgcgctattt ctaagtgctg caatagcact tggtgtatta
     3601 gttgtaactt cttttgtaag aacattgaat cttttgaaaa tcagaaaaaa ctttattcct
     3661 tacagaaaat caaaataaaa atataaaaat agcaatgtac ttaaactcta caaatacgta
     3721 ttgagtattt atttaaaaag cacattgcta ttttttaatt ataattatct taaaggtcaa
     3781 ttaatattct tgttattact ggtataaatt ctctactgtc tctaaatttt ttgtcattct
     3841 tctttaagta taaataacag attattgtaa gacaaattcc aactaatcca cttatggctt
     3901 caactccagt tgtcattcca attgccccca atatataata agtccctatt gttcctacaa
     3961 gaagtgctat aagaggtatt atataagcca ttacagctgc ttttaataca ttcatattat
     4021 ccatactcac ttctacatgg tcaccaactt ttgcaccaat tgtgttgtct acttctacta
     4081 atatttcttt tgactctgct gaatttgtag tagcacattt tccacaagca gcacaagctg
     4141 aatgtctttg catttttaac tttgcagttc tatcatcaac aatttccata ataaatcctt
     4201 gttgattcat aactaaccgc ctccttattt tgtaaattta tttatagtat ccataagctc
     4261 ttttatcatc tcttcagttt tatcagcatc atcatgttta atgctttctg ctacacatcc
     4321 ttttatatga ttctctaaga taattgtgcc tactctattt atagcagacc ttatagcaga
     4381 tatttgaacc aatatatcaa cacaataacg ttcttgttcc accatctttt gtatcccctt
     4441 aacttgacct tctattcttt ttaatctttt tatgagagct tccctctcat tgtttggtgc
     4501 taattttctt tcttccataa actcacctca tactctaatg atgtcttata caattatgct
     4561 ctacttacca acttgtttaa aatataatac ggttattatt actataaatg ccaaaattga
     4621 tattgcaata gacctacttt gattcttagt ttgttccata cttaactttc cattcatctt
     4681 aaatcctgcc aatgggaata aaaatataac cagtgcatca attccaagta caacttgggc
     4741 tgcaatagtc catttactca aaaagcagaa tattccgccc acacctaaaa gaacctctaa
     4801 tatatttata aacttatatg taggtttttt tgctatatat tcttcaattt tttccttaca
     4861 ttctgcacga catggatata tattcatatt gtcatcatat tttatagcta atttattatt
     4921 tggtgacttt atatccctat tacagtaaag acattttttc attttatatt ctcctctagt
     4981 cttccttaaa aaattttgcc taaattatta tacgctctaa tactattata ccaaatagat
     5041 acaaaaaaca aaaataaaag gaataatttt agctacttct tttacttcaa tgtgtagtct
     5101 ataataaatt tataataata attaaataat attatttaat ctaatttaat tattatgtaa
     5161 tctaaaataa gtcaaatttg tatgagaggt taaaatgaaa caaatggaat tagctattgt
     5221 aagtttaaaa aaagacgctg gagaaattta tgaaaatcaa ataaggcaat tcttaggtga
     5281 taatctaaag ataaatttat actcatttga agagggaaat ttaaagtttt ttaaagaaaa
     5341 acttatactc ctatctgctt atttaaaata cgatgaaata gtaaaattat ctcactatga
     5401 tgctcaaatc attgttccca agttgacatt tgaaaagaac agtattgata tgatatccaa
     5461 attagaaaaa gataaaatta tttatgtata taacttaagt aaagatatgg caatagaaac
     5521 aatttcttta atacatagat taggaataga caatataaat atcctgccct gctatccaga
     5581 gattgagttt actcccacag atgctgtaat cctaactcct ggcgaaaaaa tcctgcctaa
     5641 gtttaaaaat tgtgaagttg tagatttaaa atacagaata atagatttaa gttgtattgt
     5701 tgaaattgct acaaaaacca aattaaaaca tctaataaaa gatgacctaa taaaaaaata
     5761 cgtagaaaaa ataattccta caagttatag tacaggtgag ttattgttag atgcaaataa
     5821 gtttgaaaga caatttgatt tgttactatc tataattgat gatggaatta tatgcacaaa
     5881 taatgatgga ataattcaat tctataatca tatagctaga aaaattttgt ctataaatgc
     5941 aaatgaaatg attgactcat ttgttggtga ttgtataaaa gacatcaatt ttcaaaacat
     6001 attgaccaat aaaactccat tttttgagaa attgattaaa ataaatcata ttgatataaa
     6061 tttagagatt aaacatatac aattaaatgt atttgatgga tttatactaa agatgacaaa
     6121 gttttctcaa ttagaaaaaa agcaagctaa attaagagca caactagtaa actctggtaa
     6181 tatcagtaaa tacacatttg atgatatatt aggttctagt atacaaacta ttaatacaaa
     6241 aaaaattgct aataaaatgg ctcaatcaaa ttcatctatc cttataattg gtgaaagtgg
     6301 tactggtaaa gaattatttg ctcaatccat tcatagtgct tctaggagaa aagatggtcc
     6361 ctttgtcgca gtaaattgta gtacttttca agaaaatcta ctgcaaagtg aattatttgg
     6421 ctatgatgaa ggggctttta ctggtgctaa aaaaggtggg aaaattggtt tatttgaatt
     6481 agccaacaat ggaactatat ttttagacga aattggagaa atggatttaa attctcaatc
     6541 taaattactt agagtcatac aagaaaaaca agtacgacgt attggttcta ataatgtcat
     6601 tgatattgat gtaagaataa ttgcagctac taatagaaat ttaaaagaac ttgtatctaa
     6661 aaatatgttt agaagagatt tatattttag gttgaatgtt cttcccctta aaatacatcc
     6721 acttagggaa agagctgctg atatatttga aatttttggt tcattaaaat atgatattcc
     6781 atgtaatttt attctaagtg aggaagtaaa agaaatcttt aaaatgtata gatgggaagg
     6841 aaatgtaaga gaattaagaa acttaggaga atacttctgt tacttgggaa aagatattat
     6901 agaaatatgt gatttacctg aatatatatt ggacactata gattcaaatt atagtagaac
     6961 cgtttgtaat aaagtaagtg ataatataaa aaagtatcaa ttcaacattg gcaaagacaa
     7021 aaacatcatg aaatacgact ataattttaa aaggtcacta gatgaataca tatttatatt
     7081 ggataattta aaaaaggctt atgatttaaa agaaagaata ggtagaaaaa gcttatgtaa
     7141 aatagcttta gaggaaaata gatttttgac agaacaacag attagaaata tgcttttaga
     7201 actacaagac tttgggcttg tagacatact tgttggtcgt ggaggttcaa ttataacatc
     7261 aaaaggagta gaatttctaa aaaatataaa taggtctaac aagttaaaca gttaaaccta
     7321 tttaacctat ttaattacat tttcatatat ttaaaaattt attagaaata accttttcca
     7381 caaagtttta agtataatta atatttttat cttatctatg ctctgaaatt acttaatatt
     7441 aaatattaac ttgtgaataa atatttttta ttatcaatat tggcacgttg tttgcttaat
     7501 attattaaca taaaattaaa attactggag gtatttacaa tgaattataa ttttaatgag
     7561 attgttgata gaagcaataa tttttcatct aaatggtcag aaatggaaaa aaagtatgga
     7621 actaatgatt tacttcctat gtgggtggct gacatggatt ttaaggctgc accttgtata
     7681 attgattcct taaaaaatag actagaacaa gagatttatg gctacacaac aagaccagat
     7741 tcttacaatg aatctatagt aaactggcta gataggagac ataactggaa aataaaaagt
     7801 gagtggttaa tttatagtcc aggtgttatc ccagctataa gtcttttaat aaatgaactc
     7861 acaaaagcaa atgataaaat aatgatacaa gaaccagttt acagcccttt taatagtgtt
     7921 gtaaaaaata acaatagaga actaattata agcccattac agaaactaga aaatggaaac
     7981 tatataatgg attatgaaga cattgaaaac aagattaaag atgtaaaatt attcatactt
     8041 tgtaatcctc ataatccagt tggtagagta tggacaaaag atgaattaaa aaaactagga
     8101 gatatttgtc ttaagcataa tgtaaaaatc atatctgatg aaatacatag tgacattata
     8161 ttaaaaaaac ataagcatat tcctatggca tctatttcta aagaatttga aaaaaataca
     8221 attacttgta tggctccaac taaaactttt aatatagctg gtcttcaatc atcatatgta
     8281 atactacctg atgaaaaaga ctataaattg ttagatgatg cttttaccag aattgatata
     8341 aagagaaata attgctttag cttagttgca actgaagctt catataataa tggtgaatct
     8401 tggctagaat catttttaga atatttagaa tctaatatag attttgctat aaaatatata
     8461 aatgaaaata tgccaaaact taaagttaga aaaccagaag gtacttattt attatgggta
     8521 gactttagtg cacttggatt aagtgatgag gaactagaaa gtgtacttgt acaaaaaggt
     8581 aaagttgctc taaatcaagg aaacagcttt ggcattggag gaagtgggta tcaaagaata
     8641 aacttagctt gtccaagaag tatgcttgaa gaagctctta taagaataaa aaatgcaatt
     8701 aaataaaaat atttaaggag aatttgcact atgaaaatga atacagaaag aaaaatacct
     8761 tcatttagat attccttgtt agtaatgctt gcaataatag ctctaacatc agttggtata
     8821 gttgtattta atgcttctat aactacaatg tttttacttt catggcttat agtagttcct
     8881 gcagccacga aacttggtta tactaatgat gaaatagaag cttttggatt tgaagttggt
     8941 aaagatgcct ttcaatcaaa cttaattata ctttctgttg gtgtactaat agcagcttgg
     9001 atagctgctg gtacaatacc aacagtagta tatagtggac taacaataat aacccctaaa
     9061 tattttctgc ttacaacatt aatcgtatgt tcattaactt ctgttgctac tggtacatct
     9121 tgggggactc ttggaacttc tggtatagca atgatgagta ttggtactag tatgggtatt
     9181 cctcttggtt taacagctgg tgcagtcatt tctggtgctt tctttggaga taagatgtca
     9241 cctctatcag attctacaaa cttagctgca gcagtttgta agacagatgt aattacacat
     9301 atgaagcata tgttttatac cacaggacct gcatacgtaa tatgtatagt tctgtataca
     9361 gtaattggat ttaagtattc aaataatact atagactatg ttcaaataaa tcaaatcaag
     9421 gatgtattaa attcaaactt tcatattggt ttagtagcaa tgattccaat aatattttta
     9481 ttattacttt tattgctaca aaagccaccc attatatcta tactaagttc agctatcatg
     9541 ggacttatta tagcagtatt tcaagaagga gaaaaaatag gtgatatttt aaattacatg
     9601 ctatcaggat ttactataga tactggattt gtctatgccg acaaactatt aaatcgtggt
     9661 ggaataatga gtatggctga aaccgtttta ctagtatttg tagttttcgt tattgccggt
     9721 attctccaaa aaactggatt cttagaagta ctgcttcaac ctcttataac taaaatagga
     9781 aaatcaagaa ctaaattggt tgggtctact tttatagtaa gctactttgc aaatgcattt
     9841 agttcatcaa tgatgtttac ttctgtattt gtcggaactc taatgtctcc aatatacaaa
     9901 gagtttaaat taaaaccaca aaatctatct agaattatag aagatacagc tacacttggt
     9961 ggtccattaa taccatggaa ttcaaatgct gttttctgtg ctcagactct tggtgtaagc
    10021 ccactagaat ttattccata ttgtttctta agttggatta cacctataat ttcatttatt
    10081 tatggtgtaa ctggttttac tatgcttaca tacacagatg atgaaattga agagttagaa
    10141 atatttgata ctgctcagag aagagttgat tgaatatgaa atttgaatac tattacttaa
    10201 ttcaggatat tgcaggtata ttattggcat ttataggact tagaatgtct attattggat
    10261 ttaggatact ttctatgaag gggatttcta taaatacact tttaattgta attaaatact
    10321 gcttatttac catagcagga ctgaatttat taatatctaa gtttggaatt agacactgga
    10381 tttggagtgt ttgtatgttg ataatatcca taataataaa tcccaggatt aaggtatcaa
    10441 aatagaaata gttattagac ttaaattttt ataaaaaaac tgctttacaa attaaagcag
    10501 ttttttattg ttacttataa ttaaataact ggtgtatcag ataagtaatc attacttaca
    10561 taaccatatc caccattata ctttattttt acccatccag tagcaaatat ttctattgtt
    10621 tctactttgc ttccatatga aaggtgacct attttttcat cttctgtact tggtcctttc
    10681 ctaacattta gaccatcttt ttcttttaca tactttattt ttatattttc attactatta
    10741 tcaggcttat tatttgtcaa ataagaccca ctaacatatc caataccttc attgtatttt
    10801 atttttaccc atccagtttt taacacatct aaaacttcta cttcatcacc attatttaga
    10861 tatcctataa cagaagcttc tattgtagca tcatttctta tatttacctt atctgtatta
    10921 gttacataca ttactctctt attgttacta tttgcctgta aatcttttaa ttctttaaat
    10981 gcattttcat ttcctccact accaacttga gttatagagg tagcattttt attcaaaaat
    11041 acactctttt gtgttgcact taattgttct cctacgatta gtacaggaga attttctttt
    11101 gatgccagta ctcctactgc tagagcgtct ataagttgat tttgattttt cattccatcc
    11161 ttagccacaa atacattgtt taatttatta gatgtataaa acttttctat tactttacca
    11221 ttagtttcat ttctatctgc tccacctatt ctttctgaat taggtaaact ttgttttatt
    11281 ttatctgata cagcattagt agagcctatt atgtatgatt tatttatatt cttagattct
    11341 ataaaatctt taaagctaga tataccagat gcatcagata ctggaagtat aaccattcca
    11401 ttagtagcag ctacagaagc tatactaact gcatctgcaa gacctgtgta cccatttact
    11461 acagctattt ctgatatatc ttttaatcct tctatttttt tagctatact caaagctgtt
    11521 tggtatctat cactaccacc aactctatca gtagttaaat tattagattc taactgtttt
    11581 tgcacatcat ttgatacaga actagataga ccaattatat aaactttttt aactcctaat
    11641 ctttttaatt ctttctcagt ttgtgaattt agattatttt tttctgtcaa taatatagga
    11701 gcatttttta atttagcaaa tggtgttaca gctaatgcat ctacaataga ttcactattt
    11761 acaactacag cctcattcgc actactccat ccttttttgc ttacttcaac agcagtttgg
    11821 tatctgtcct gacctactaa agtattctcc actgttgttc tacttatttc atttgcatat
    11881 gctactggtg ataagcttaa aactagagct aagcatagca ttgatgatat tgatttagtt
    11941 aatttcatct tgactccccc taaaattata gtatctaaat gtttcctatt taaaatagta
    12001 taacataata atttaagtaa catcattatt ataaaatatt atttttctat ctaataagct
    12061 tttattctta cttgacatta atactttact catcaaatga attttttata taactaaagt
    12121 ttgtaacaac atttccttag ttaatcattt aaagctactt tttttaatta gattatgtac
    12181 aaaatataaa taacaattta aataactttt taatatattg tagttgatta tataaacttt
    12241 atattataat gtataatgtg gagttgatta taaaattgat ataactcaag tcatattaaa
    12301 agaggtgtat tttatactat ggatttattg catcttaagt attttcaaac tgttgctaga
    12361 atggagcaca taacaaaagc atcacacaag ttaaatatag cacagccagc tttaagcaaa
    12421 actatatctt ccttagaaaa agaacttgga gttcagttat ttgacagaaa aggaagatat
    12481 attgttttaa atgagtatgg aagattgttt ctaaaaagag tagatagcat tttagattta
    12541 gtagaaagtt caaaaaagga gttacaagac acttctttag aaaattcagg agaagtaaag
    12601 atattatctc ctgcagcggc taatgtgtta ccatcacttt tgagtaattt cagaaaacta
    12661 tatcccaata ttacttttaa tgtgtcacat actttgccat catcttataa aaaaagtgat
    12721 tttgatttgt acatatcttc ttcatttaca aagctaaact cagaaaatag cataacttta
    12781 acatgcgaag aaatactatt aggagtttca ataaaccatc ctttatcatt aaaggatgaa
    12841 gtatatctaa gcgaagtttc agatgaaaac tttatagtta taacgaaggg tgagaattat
    12901 agagaggtaa ttgatatcct gtgcgaatca gctaatttca aacccaaaat agcatttgag
    12961 tctgattcac catatacaat ttatgctctt ataaaatcac ttcaaggagt tggatttata
    13021 tgtggaaaat catggggatt atctcaagac cctgagatta agttgttaca tatcaaagat
    13081 attgaattta aaagatattt aaatctttca tggtttagcg aaaactatga atctaaagct
    13141 gtgttgttgt tcaaaaactt tcttatcaac tattttaaaa atatttaaat caaagttgat
    13201 aatattaaag aggatatatc aaaatataat
//
LOCUS       DAEPOZ010000108        13197 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.108, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000108 DAEPOZ010000000
VERSION     DAEPOZ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13197
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.108"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(35..814)
                     /locus_tag="KR440_002203"
     CDS             complement(35..814)
                     /locus_tag="KR440_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699250.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKALDKENNVLGKSKALSFEYKNGNDEISLDTANNVLEKS
                     SDNLKVVDTYKDINISYEAFAQKFVPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNEFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(811..1290)
                     /locus_tag="KR440_002204"
     CDS             complement(811..1290)
                     /locus_tag="KR440_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH0699251.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRTLLKTKLENTEVII"
     gene            2096..2797
                     /locus_tag="KR440_002205"
     CDS             2096..2797
                     /locus_tag="KR440_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBH0699252.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKSALKEASNLGIE
                     RKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLLVVFILGHRMRGIVEKMNVKYNPTW
                     VKKLLAGATVGLFAYLLSNQIKTFEIPKLTPAVISAIVMLVLTTLFKKNQRLQELSLG
                     IAMLIGMFGTQIVLS"
     gene            2823..3479
                     /locus_tag="KR440_002206"
     CDS             2823..3479
                     /locus_tag="KR440_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699253.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGLRFGQMPMLSVIFQI
                     WGLVATTYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFITDSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLGGILITRVFFVIDSNKTKE
                     "
     gene            3686..5326
                     /locus_tag="KR440_002207"
     CDS             3686..5326
                     /locus_tag="KR440_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBH0699254.1"
                     /translation="MFDVKIANGIIIDGTGNSRYKGDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPIKLNLHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHISHLKVMGHKNFGTIDKVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWDGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAGGKQTDVCAGRVIKNPLSIAK"
     gene            5685..7007
                     /locus_tag="KR440_002208"
     CDS             5685..7007
                     /locus_tag="KR440_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699255.1"
                     /translation="MKILYVGPGMVIDKILAVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGRVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKFDISRL
                     SIDTYDKAMLKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDICCC
                     ITGLEEAYHAMKKENIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIVVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPAVKEKNSFDERFYQIATETGLSANTVYKVFNKIINSGKTEFTSKELAI
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            7204..8211
                     /locus_tag="KR440_002209"
     CDS             7204..8211
                     /locus_tag="KR440_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBH0699256.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDK
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGTMGCPPEILKKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            8249..9646
                     /locus_tag="KR440_002210"
     CDS             8249..9646
                     /locus_tag="KR440_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HBH0699257.1"
                     /translation="MASTNKNNVTSKEEEKEVSTSGLESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKGNIDKINTNKSDINKVNIDTVDTDKLNTNTLTEKNKKVIFKGEDN
                     MSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDYIMKITHNIWE
                     EKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIGQNVIWSNFGA
                     DGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEWLVRDNLWIVT
                     QLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDDSVGEMMLEMT
                     SRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLLASIPNGSYEVERVT
                     CNQREKNEGYDVSIRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQGKVKEEWITF
                     DGMDVLKQMYMGVEE"
     gene            9791..11056
                     /locus_tag="KR440_002211"
     CDS             9791..11056
                     /locus_tag="KR440_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0699258.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTDIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAG
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            11098..12137
                     /locus_tag="KR440_002212"
                     /pseudo
     CDS             11098..12137
                     /locus_tag="KR440_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
     gene            12172..13194
                     /locus_tag="KR440_002213"
     CDS             12172..13194
                     /locus_tag="KR440_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBH0699259.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMVAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
BASE COUNT         4679 a         1544 c         2510 g         4464 t
ORIGIN      
        1 aataaactca taaacaactt attattactt atgtttattt tgcatctatt acttcatgag
       61 ccattttaac taagtcattc atagataaat ttgagtcttg aactgtaaaa gaatagtaaa
      121 ctccatcttg tttccatgtc aaaaacttaa attcattaac ttcaactttg tctgattcat
      181 tatttggccc tacactaaat gtgtatttgc ctgatttttc atcaatttta tcctgttctg
      241 tcattttata accttctggt acaaactttt gtgcgaatgc ttcataagaa atattaatat
      301 ctttgtaagt atctacaact tttagattgt cagaactctt ctcaagtaca ttatttgctg
      361 tgtcaagaga tatttcatca ttgccatttt tatattcaaa actaagtgct tttgattttc
      421 ctaaaacatt attttctttg tcaagtgctt ttttatttac agtatatgct cctttaaatt
      481 tatatccatt actaaattca gaaactatat taggggtaaa gttaaagtct ttttttactt
      541 gttctgtagt tgggatagat gtataggtag ccttagtact tgattttcca actaaactat
      601 atatatgtcc tactgcaagg gctgttgtgc tcaatatcaa agtaatggct aaagttgcaa
      661 ctaagccttt tctaaatggt aatttgtgtg ttttattatt aatcatttta taattttcct
      721 ctctttctgg taaactattt agtgttttac ttaaactatt atgaaaactt tctggaactt
      781 ctggaaacga attatttaat atatttttat tcatatgatt acctccgtgt tttctaattt
      841 agtttttaat aatgttcttc ctcttgataa tctacttttt acagttcctt gaggtatatc
      901 taatatttcc ttaacctcat ctactgaata accttcaata tagtaaagga ctattggaat
      961 tttaattttt ttaggtagac tttgaatagc agtatacaaa tcgctatagt catctttttc
     1021 atcaattttt ttgtttttga agacatcttc aaatgataga gattttaatc gtttgcgtct
     1081 taatgaatta cattcattta tgactattct aatcagccat gttttaaagt attgttcttc
     1141 cttaagagaa tccaactttt catatgcttt taaaatagca ttgttgacgg catcttcaca
     1201 gtcttggtcg tttcctagaa tagacttaga tactcgatac aaagtctgtt ctgattcaag
     1261 tatattgttt gtaaatgtag ttttatccaa ctgagttcct tcccttctat gcaaatcgta
     1321 gcgctacttt tttgcctttc acttattaga taaaatttta ctatagatgg ttcacttttt
     1381 ttatgattat ttgataaatt taagtttgaa agttttgcct attctataca aatattctat
     1441 aatatatttt tttctagtat tttcccctca tatataaagc catgtagata tattaaactt
     1501 tagaagccat ttatatattt agttttatag taagtgttta tattaagtaa ttataaaata
     1561 gtaagtattt gatgttgagt agttagaaaa taatgctttt ggattattta cttcatgtat
     1621 agatttaaaa taagtttttt aattaatgac taaagataac ggtattttga ctaaaagtag
     1681 gcttttaaga aaaaacaatg aaacttaaat gagaatcaag agttagaaat gtcattattt
     1741 tagatagata tgaacataaa taaacttaaa aaatataaat tataaaaata ataatttttt
     1801 taaaataaca aagttttttg attaaaagtt ttatattgaa atgtcaaagt aataaaatac
     1861 cttataagtt aattacaggt taaaaaaata atatgtgaat tttctgatat ttactaatta
     1921 tcaataaagt atttaagtat taatgttttt agtgagactt taagacagat aatctcctat
     1981 taaagatact atatcttaga taaaataaat taaaacaata taaaaacata acaagattac
     2041 agaaagaaag ctcataataa aacactaaaa aatttatttt aaggaggaat aaagtatgaa
     2101 gtttcaagaa atgattaata gcccaggtct ttggattgtt tctagttttt tagtaattat
     2161 ttcagttgtt caagcaatcg tatttatgaa atcagcactt aaagaggctt caaatcttgg
     2221 tattgaaaga aaaggaatta aagcagcaat tcgctcagct agtgttacag caattggacc
     2281 ctctctatct cctgttatta cattattatc actggtagct gtgattggtg ctcctacgac
     2341 ttggatgagg ctttgtgatg taggagctgc cagaacagaa cttggagtaa tatctcttac
     2401 atcaaattta tctggagtag aagtaggtag tgctgcattt ggagcagaag ctttttcgta
     2461 tgcactatgg ggaatggctt taaataactt aggttggttg cttgttgtat ttattttggg
     2521 tcatagaatg cgtggaattg ttgaaaaaat gaatgtgaaa tataatccta cttgggttaa
     2581 aaaactactt gcaggggcta cagttggatt atttgcttac cttctttcaa atcaaataaa
     2641 aacattcgaa ataccaaaac ttacaccagc agttatttca gctatagtta tgctagtatt
     2701 gacgacttta tttaaaaaga atcaacgatt acaagaatta tccttaggta ttgccatgtt
     2761 gattggtatg tttggcacac agattgtact atcatgatta aaacagataa gaggtgataa
     2821 ttatgtatga taaaaagatg ataaaaatag ggagaatttc tataggtctt gcaattattg
     2881 caaattttat tcctgcaatc tatgtaggtc ttcgctttgg tcagatgcca atgctttctg
     2941 ttatattcca aatttgggga ttagttgcta caacttatgg agtgagttgg attgttcaac
     3001 ctatagctta ttatccaaca ctaggagctc caggttctta tataggttgg cttgcaggtt
     3061 ctgttggaga tatacgtatg cctgcagcat caatggcaca gaaaattgca ggtgtagaag
     3121 caggaactca tgagggtgaa gttgtaggaa caattggtac atgttgttca atctttgtat
     3181 cagcttctat gattactatt tttaccattg taggctataa agtaattcct ttgttacctg
     3241 aatttattac tgattcattt aattatatac ttccagcatt gtttggggca atttacgttg
     3301 atattgcgcg taaagatatt cgagctggtg gatgtacgat tgctgtagca ttagctatta
     3361 tgtactttgg tgaaaaaatt ggtataccag gtggattgtt gacattgttt attgtgttag
     3421 gtgggatact tattacaagg gttttctttg taattgatag caataaaacg aaagaatagg
     3481 attgtttact aggaagaaag actagaatta tttgctgaga aaaagaatag gattgtttac
     3541 taggaggaaa gactagaatt atttgctgag aaaaagaata ggattgttta ctaggaggaa
     3601 agactagaat tatttgttga gaaaaaagaa taggattgtt tattaagaaa tatgaatata
     3661 aataagtctt gaaaggagaa gaagaatgtt tgatgttaaa attgccaatg gaataatcat
     3721 tgatggtaca ggaaattcaa gatataaagg tgatgtaggg attgtaggag acaaaatcat
     3781 ggctattgga gatctaagtc aaaaagaggc taaagagact atagacgcaa ctggaaaatt
     3841 tgtatcacct ggttttatag atttccacac tcattctgat ttatctcttg tgtatgacaa
     3901 atacacaaga agccgtatcc atacaggtgt aacaacagac gttattgcaa actgtggtat
     3961 aggagttgct ccgataagag aagagaagaa gcaggagtta attgactatc ttggaacgag
     4021 gataattggt acaattccaa taaaattgaa ccttcactgg aatacaatgc aggaatactt
     4081 tgattactta actgaaaatt ctccagcagt aaatgttgta gcttatgtag cacaaggacc
     4141 tgtacgtatc aatgaaatgg gattttcaaa ggagccagct actttagagc aacttaaaaa
     4201 tatgaagttg gaagttagaa aggctatgga agctggatgt gtagctcttt cgtcaggatt
     4261 agtttattta ccaggagcat atacaaagaa agaagaaatg gctgaattat gtaaggaatt
     4321 gataccatac aatggatact atatatctca tattagagat gagggtgatg aagaaatgga
     4381 agctcttgat gaagcaattt atatagctaa gacggcagga gttcctctac atatttctca
     4441 tttaaaagtc atgggtcata agaattttgg aactattgat aaggtattta aaaagctaga
     4501 tgaagctgag gcagatggct tggaagttac ttttgattgt tacccatata cagctggaat
     4561 gacatctttg ggtgcacttt tacctccttg ggcttttgaa ggtggtgtag aaaacatggt
     4621 aaaacgatta gaagtgcaag aaaataggga cagaattata aaagagctag aggaagggat
     4681 tcctggatgg caatgtttct accagttagc aggtggttgg gacggtgttg tactagcctc
     4741 tgtaatgact gaagcaaata aatatgtaga gggtaaaact ttaatggagg tagctaaaat
     4801 caatggtgaa aatccatttg atacgttctt taggcttttg attgaagaaa aaagtaaaat
     4861 tcaagtagtt gttcatacac aaggtcaaga ggatacagat aaggtcgttt gccatccaaa
     4921 gtcatgtata ggttctgata gtatggacct ttctacagaa ggcttacttt ctcttggaaa
     4981 gcctcatccc cgtgcatttg gtacttttgg acgaattttc tcttactatg ttagagaaaa
     5041 aggtatgttg acttttgagg aagcagttaa aaagataact tatctttcag caaaacgctt
     5101 aggaatttat aaagaaaggg gtctacttaa ggaaaattat tttgctgaca ttgtggtatt
     5161 tgacccagat acaatagaag ataaagcgac ttatagtaat cctaaacagt atacagtggg
     5221 agtggaatat gtattggtaa atggtaaaat tgcattggca ggaggaaagc aaactgatgt
     5281 atgtgctgga cgtgtaatta aaaatcctct tagcatagca aaatagaata cattaagaac
     5341 ttttataaaa atagtctttc tttgaatatt aaggaaggct atttttgtta aattaataaa
     5401 gattactcta cttgtgtttt ctagagaatt taactttaag tagaaaaaac aatatattaa
     5461 ttgtaaaatg taattaatat taatggataa gatttaaata caaaaactta aatacaaaaa
     5521 tgttttaaca gatagtcata attaaaaaac ttaaagtaac tttttatata agatataatt
     5581 gattatgcaa aattttatga agcagcaacc ttgttacttt aagtcaaggg aggttcagaa
     5641 agtgtcttaa gagataatag tatctttagg aaaggagaaa cgaaatgaaa atactttatg
     5701 taggaccagg catggtaatc gacaaaatac tagcagtaag taaaaagaat tttcccaata
     5761 ttgaagcaga ttttcttaga tatgatagat atattgaagt acctgattta ttggaaaaat
     5821 atagaggtag agtagatgca gttcttttta caggtaaatc gccatttaaa ttttttgaaa
     5881 aaagacaaaa agattttgtg ttgtctgatt atattcctag acatgaaaca acattataca
     5941 gagtattgct tgaaattaca tatttgctta aattcgatat tagtagactt agtatagata
     6001 cttatgataa agctatgctg aagagattgt ataaagaaat tggagttgaa tttaaggatg
     6061 aacatatgtt ttttgcagag caaaagtatt tagaagagga ctacataaag tttgtgcttg
     6121 aatttcataa atataattat caaaataatg acatatgttg ttgtattaca ggtcttgaag
     6181 aagcatatca tgcaatgaaa aaagagaata ttccagtagt tttagccttg ccttctgagg
     6241 atgtaatggt acaaagtata aaaaacattc agatgcgtta tattgcaaag aaaaacaatg
     6301 agaatcagat tgttgttctt gctatcaaac ttaatatgcc ttcagaatat tcactgatga
     6361 aggaagatga gtacgcttat ctttcacaaa ggattaagat tcttgataaa ctataccatt
     6421 tcaatagccg tatagatggt gttttggtag agcagagtag aagcgaattt atgattttta
     6481 caactaaaaa aatacttgag ttagaaacaa agaagtacag agatatttat ttgttggata
     6541 tgattcgtga agtttcagtt ataaacactt atataggaat tggttatgga aaaacagcaa
     6601 atgaagctaa atttaacgct tatgagagta ttaagatggc tcaaagtcaa aaaaatgatg
     6661 cagcatatgt agtttttgaa aatggagaaa ttatgggacc actaaagcca aatcctgctg
     6721 tcaaagagaa aaatagcttt gatgaacgat tttatcaaat tgctacagag acagggctaa
     6781 gtgctaatac tgtatataaa gtgttcaata aaataataaa tagtggaaaa actgaattta
     6841 catcaaagga attagctatt atatgtggtg taagtattag aactatggac aggattatac
     6901 ttagattgtg tgattctggt tattgtgagg tagtgagtga acagcttatg agcaagtatg
     6961 gtagacccag tagaatatta cgttttaaac cttttctgtt gttttaaata gatataagtt
     7021 aaaaattaaa tttttatgat aacattttct ttacaatttt ttagatatat gttaatattt
     7081 agaaaaatgc atatgtattc ataaaataaa ctcaaaatag ctaaaaaatc attaaaaagg
     7141 aaatcgtatg cataaaatga gaaagttaat ttataactta aataaatctg ggaggagata
     7201 attatgggaa aatatcagga ttcaaaaaaa gttgtaagaa attattttga agcattagaa
     7261 aatgctactg gggatgaggt tgaaaatgtc ttaaagcaac atatgaaatc agattataac
     7321 tggaaaggtg tgtatccatt tcgtgaacaa gaaggcactg aaaatgtagc agatgtattt
     7381 tggaagcctt taaaaaaatc attgagtaat atgcaaagac gacaagatat tttcattgca
     7441 ggaacaaatg agattgacaa tagtgaatgg gtaatgagta tggggcattt tatgggactt
     7501 tttgataagg attggcttgg cattaaacat actcgtaaaa tgattagttt aagatatgca
     7561 gaatttaact gtgttcaaga tggtaaaatt gctaaaactg gattatttgt agatataatt
     7621 gggtttatga ttcaagcagg agttaatcca ttgccaccac aaacaggttc atattttgta
     7681 tatccagggc caattgacca taatggatta ctttttgaag atgcagatga agttgaagga
     7741 gtaaaaactt tagcattagt aaataaaatg gtagatgatt tatctgaatt aaatgaaagt
     7801 ggtactatgg gatgcccacc agaaatactt aaaaaatctt gggctaaaga tatgatttgg
     7861 tatggtccag gtggaattgg tgcttcttat actataccta gatatcaaga gcaacaccag
     7921 ctaccattta gaaataattt aaaaggaaag actttcaatg ggcatgtatg tagatttgca
     7981 gaaggtaatt ttgcttgttt ctttggatgg cctaatctga ctaatgtacc atgtggaggt
     8041 tttttaggat taccaggtgg agaggttaaa gcagatatgc aagttgttga tgtttattat
     8101 cgtaagggcg ataaacttca agaaaactgg gtgttaattg atattcctta ttggttgaaa
     8161 caacaagggc ttgatattct tgagagaact aaaagtattt ttaatgctta gttatgcata
     8221 cgtattctaa aacaaagggg gatataaagt ggcatctacc aacaaaaata atgtaacaag
     8281 taaagaggaa gaaaaagaag tttctacaag tgggcttgaa tcatgtacgg ttaaagaaaa
     8341 caaagctaaa atgaatcaaa ctaattttag taatataaat gaaaatcaaa caaatgagaa
     8401 taaaacgaac acatataaag gaaatataga taaaataaac acaaataaat ccgacataaa
     8461 taaagttaac atagatacag tggatacaga taaattaaat acaaatactc ttactgaaaa
     8521 aaataaaaaa gttatattta agggggaaga taatatgtca tctacaaaaa tttactatgg
     8581 agattcatta gaagtaacgc ctgttggagt aatggattac aatgattttt caaaacagac
     8641 aaaaagggaa caggaaatac ctggatttga ttctaagtat agagattttg tagattatat
     8701 tatgaagatt acacataata tctgggaaga aaaaggtata ggtgttattt atgatacata
     8761 tcacaataat gttacaatgc attgtggctc ttcaaattta gttggtatta aagatgttat
     8821 ttccaatact cttcaaacat tacatgcgtt tcctgataga agattaattg gtcaaaatgt
     8881 aatatggtct aactttggtg ctgatggttt tttatcttct caccgtgttt tgtctacagc
     8941 aactaactta ggtgatagta attttggacc agcaacaggt agaaaaataa actttagaac
     9001 tgtaattgat tgtgccacaa ctaacaatag aatccacgaa gaatggttgg ttagagataa
     9061 tttatggatt gttactcaat taggattaaa ccctcaggaa gtagcaaaga gtatggcaaa
     9121 ggcaagtgaa tctaaagtgc ttagcttaca atcaacttat ggtatctgtg aatctatgga
     9181 tggtcaattt atgccaacta agtatcaagc aaaagatgat tctgttggcg aaatgatgct
     9241 tgaaatgaca agccgtattt ataattataa atatataaat gaagttaaaa aatactatca
     9301 tgataatgca gtagtacatt ttatatgtga taaagattta aatggttatg atgagattca
     9361 aggaatgatt gtaagtttac ttgcatcaat tccaaatgga agttatgaag tggaaagagt
     9421 aacttgtaat caaagagaaa aaaatgaagg ttatgatgta tctataagat ggagattacg
     9481 tggaataaat gaaggtatag gattttttgg acagccttct ggaaagcata tagaagtaat
     9541 gggaatcaat cattatcata tacttcaagg taaagtaaag gaagagtgga taacttttga
     9601 tggtatggat gtattaaaac aaatgtatat gggagtagaa gaataaagct gtttagagta
     9661 taaataattt tgaaatgtat atagggaagt attgctttat aaaatttaag tggggaatgt
     9721 atatgaaaaa tagatgttta aacttttagc aaaaattatg actaaattta aaagggggaa
     9781 ttataaaaat atgaaaacaa cggttgaaat agaaaatgat atcatattag gaaaagacta
     9841 ttcaaaccta aaaagaggaa ttgcattttt ttcaattgct ttaatatact ttttttattg
     9901 ctacaatttt atggtgggta cttttattaa accaacaatg atttatgctt tagcagatgg
     9961 gggatttggc ttttcattaa aacaaacaga agaaatattt gcagttatgt cttttggtac
    10021 aattccaggt acatttattt ttggggtgat ttctacaaaa attgggaaga agaggacttt
    10081 aattagcgtt gctttactga ttggattgac aacatttata ccaatgttat ctcctacaga
    10141 tattatgtta tggaaaatag cacgtttgtg tacaggagta gtgttgggag gcgtatttgg
    10201 aacagctatg cctttagttg cagatatgtt tccaagtaaa tatcgtggta agttagctgc
    10261 tatattaact gctttatttt cattagcaat gatttttggt gggaaagtat atggcgtttt
    10321 aggtgatgca aattggcaaa tattgatgta tactgcgatt ataccaccta tagttggagc
    10381 tatcttagca atcttctttg tgccagatga tttggagtat acaaaggaat tagttaaaaa
    10441 gggaaaagaa actggtgaaa agattagcta tataagtatg tataagggaa aatacttatg
    10501 gattggattg ggaacgattc ttttatcagg tgcaaatttt acagcatatt cagcattttc
    10561 aaataatgca actacatacc tagtgacagg aataggaatg tcagcagcag tggctgggtc
    10621 tatatatagt ttacaaggaa ttggtcaatt agtaggatat ttattctggg gttcaattgc
    10681 agataaattt ggaagaaaaa taccttttat aggtatggca ttagcagcag tatttgtttt
    10741 tatatataca aaattaggtg gagataatac aacaacattc tatatgatat cagtattact
    10801 aggtgttgct gttggatatt ctggagcatg gggagcatac tacacagagt tgtttccaag
    10861 taaatatcgt tcgttgtcat ctggtatgtc atttaatgga ggaaggatta tatctacgtt
    10921 tgcaatacct gctatagctg gtacagcttc tggtacttta ggtatgatga ctatctttta
    10981 catatcaatc gcagtctttg tagcaggtgc tattgtatgg ttattcttac cagagacgtt
    11041 aaataataaa aaataaaata ctaggaattt attttgttac ttagaaaggg gtggcttatg
    11101 atttcatcaa ttgatgtatg tattataata ggatatttgc tattaatgtt agttattggt
    11161 tattattcag gaaaagataa taaaaatcaa gaagattatt tcttggcagg taggtcaatg
    11221 ccatggattc caattgcttt atcagttgca gctactatga ttagtgcaaa tggctttata
    11281 ggtggtcctg gatgggcata tgtagatgga atgtatccag taatgtaaaa tgtgcaaaaa
    11341 attcttttgt agttagtgca tttgttatga atttagttta ctactttatg ctattggtag
    11401 gagtaatttt atttgttttt tatgggggaa ggacatttga aacttcaaat gaaataatga
    11461 ttacttttat tttaaatgaa cttccagtag gagccattgg aattattatt gcaggtgtgt
    11521 ttgcagcagc aatgtctagt atcgactcta tattaaacag tatgacaact gtttttacga
    11581 aggatattta tgagttttat ttcaaaaaag acaacaaaga atcatcatta aaagttacaa
    11641 tgattataac tgtagttata ggagttgtta tgacaggttt tattattatg ggatttggag
    11701 gaagcatcaa atcaattctt gaccttgttg gtaactatat ctcatacttt gctggaccag
    11761 ctacaggtgc atttgtacta gcaatgttta catataaagc taatgataaa ggagtatcta
    11821 ttggttttat tgtagggtta gttttaggat tttttatttc taataaatat aatgtgagtt
    11881 ggttgtggaa tccagcaata ggtgctacaa taacattgat atttggatat atttttagtg
    11941 tcattttaaa gaaaaatcat aaagtagatg atattaaaaa atacactgca tttggtatga
    12001 gaaatcacct tatttctcaa ggaatggata aagtagatgg agtatctata gtgccatttt
    12061 ctttaggtaa acaagaacta attgtattag ctttttttat tatccaatat attatattat
    12121 ttttgataca attttaagaa gaaatactag atatttaagg agaaatacta aatgaatcaa
    12181 aagaaagttc attataataa atcggctaca tcaaaagatg tcgcaagatt ggcaggagta
    12241 tctcaatcat ctgtttcaag agcatttggt agtgcaaatg gaaagggcgt aaaacctgaa
    12301 gtaagagaaa gaatttttcg tgtagcaaac gaaatagggt atgttcctaa tttggtagca
    12361 cggggaatga ttagtggaaa aactaatgtt ataggtttga tagttggtga tagtctaggt
    12421 ccattttaca atagaattat caatttattt gtagaaaaaa ttcaagagat aggtaaacag
    12481 tgtctcgttt ttaaagttcc tagacaacaa agtatagata gtataatttc aaaagtaatt
    12541 cagtttcaag tagaagctgt gattattaca gcttcggcca tgaataaggt gatggctgag
    12601 acttgtgaag aaaacaatat tcctgtgatt ctttttaatc gttttatacc tggtataaaa
    12661 ataagtacag tctatgtaga cccaattgaa ggtgggggca tggttgctga atatctgctt
    12721 aagaaaggtc ataagaatat aggatatatt caatttacga gagaaacaag tgaagaaatg
    12781 gaaaagaaaa ttggatttta ttctaagtta agacaaagtg ggattcataa ccttaaagaa
    12841 gaatctgcaa actatgatta tgattctgga tatgaagcag gtaagagaat gttagcatta
    12901 tcaaatcctc caacagcaat tttttgcaca agtgacttga tagctattgg tgtaatggat
    12961 gctgcaaggt ttgagtacaa gttgagggtg ccagaggatt tatcagtgat aggatatgat
    13021 gatattcaaa tggcttcatg gaagagttat aatttaacga cgattagaca accactagat
    13081 ttgttgattg aaaaaacaat taacatactt aagtccttat taaatgaaga aaatccagag
    13141 tcagtcgttg aaatgttaaa acctgaattg attgaaagag gttctacact ttaagtg
//
LOCUS       DAEPOZ010000109        13111 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.109, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000109 DAEPOZ010000000
VERSION     DAEPOZ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13111
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.109"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(35..2035)
                     /locus_tag="KR440_002214"
     CDS             complement(35..2035)
                     /locus_tag="KR440_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0699260.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKEKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSDLSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNVRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTKKEFEEILRILYERRESSSGVGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            complement(2066..3784)
                     /locus_tag="KR440_002215"
     CDS             complement(2066..3784)
                     /locus_tag="KR440_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBH0699261.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKYDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLNIGSYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMKSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGEEVADIIDNIRVAIANKKWNDDVTVTISAGVAHISDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            complement(4010..5248)
                     /gene="fabF"
                     /locus_tag="KR440_002216"
     CDS             complement(4010..5248)
                     /gene="fabF"
                     /locus_tag="KR440_002216"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBH0699262.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            complement(5277..5501)
                     /gene="acpP"
                     /locus_tag="KR440_002217"
     CDS             complement(5277..5501)
                     /gene="acpP"
                     /locus_tag="KR440_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBH0699263.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            complement(5555..6304)
                     /gene="fabG"
                     /locus_tag="KR440_002218"
     CDS             complement(5555..6304)
                     /gene="fabG"
                     /locus_tag="KR440_002218"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBH0699264.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTRVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            complement(6304..7254)
                     /gene="fabD"
                     /locus_tag="KR440_002219"
     CDS             complement(6304..7254)
                     /gene="fabD"
                     /locus_tag="KR440_002219"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBH0699265.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNAVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPAGVGAMAAILGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIEILLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            complement(7270..8199)
                     /gene="fabK"
                     /locus_tag="KR440_002220"
     CDS             complement(7270..8199)
                     /gene="fabK"
                     /locus_tag="KR440_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HBH0699266.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            complement(8308..9294)
                     /locus_tag="KR440_002221"
     CDS             complement(8308..9294)
                     /locus_tag="KR440_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBH0699267.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            complement(9320..10342)
                     /gene="plsX"
                     /locus_tag="KR440_002222"
     CDS             complement(9320..10342)
                     /gene="plsX"
                     /locus_tag="KR440_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBH0699268.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            complement(10353..10910)
                     /gene="fapR"
                     /locus_tag="KR440_002223"
     CDS             complement(10353..10910)
                     /gene="fapR"
                     /locus_tag="KR440_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HBH0699269.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            complement(11117..11293)
                     /gene="rpmF"
                     /locus_tag="KR440_002224"
     CDS             complement(11117..11293)
                     /gene="rpmF"
                     /locus_tag="KR440_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBH0699270.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            complement(11318..11842)
                     /locus_tag="KR440_002225"
     CDS             complement(11318..11842)
                     /locus_tag="KR440_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBH0699271.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDTCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            complement(11935..>13111)
                     /locus_tag="KR440_002226"
     CDS             complement(11935..>13111)
                     /locus_tag="KR440_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBH0699272.1"
                     /translation="CGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHEKAGRDDK
                     YVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFATSVVITPE
                     VEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSSYLYGLPH
                     ELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAAVDGGKCV
                     DTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVYGMTGISS
                     DFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVGENGKAIR
                     ADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARDTLRLVK"
BASE COUNT         3849 a         2604 c         1368 g         5290 t
ORIGIN      
        1 tcaaaatatg tgatttcaat atcaaatact tagcttaatt tatattatta taaagtttta
       61 tcccctctga ggttatttga cttcctcctc taccagattt agatgatata tatccacatt
      121 cacttaaata agatagcacc tttctaactt tgctttcaga aatctcaatc ccttcctgta
      181 taagcttttc gattataaac cctcttccaa ctccagagga tgactctctt ctttcatata
      241 gaatcctcag tatttcctca aattcttttt ttgtataacc aaatccatct tcataattgt
      301 tagcacattc tacccttata tatggtggta agtcatctct aaaaaccata ctatcacaca
      361 ttagacttac ataagaagca acattttgta actctcttat atttcctggc caattatatt
      421 tttctaaaat agttataact tctggcgaaa gtcttacatt gtttttcaaa aagaaattca
      481 taagtgttat tatatcttct tttctattcc tcaatgatgg tacatctatt ggcaatacat
      541 ttagtctata atacaggtcc tctctaaatt ttccttctct aatcatcttt agtaagtttt
      601 tgtttgttgc tgctatgacc ctaacatcta tgtttataac attatgggaa cctataggca
      661 taacctgctt ttcttgtaat acccttagta actttgtctg tagcaatgga ggcatatctc
      721 caatttcatc cagaaatata gttccattat tagcaagttc aaatagaccc ttttttcctt
      781 ctcttaaagc tcctgtaaaa gagccttttt catatccaaa cagttgactt tctaatagac
      841 tatctggaac tgctgcacaa ttaaccgcta taaatggttg attacttcta ttggaagcat
      901 tatgaattga ttgtgataat aattctttac cagtaccact ttctcctatt attaatacag
      961 aaaggtctga tttagatatt tttttagcca attctacaca ctttaccata tttttatttt
     1021 tagtttttat atctttaaat gtatatcttg ctatctgacc ttgttctctt aatttattag
     1081 taagattttg ttctaatttt tttatatacg ttatttcttg taagttataa tatatcccag
     1141 ctttattccc catagaaaaa atactttttt tatttacatt tatatatttt ttatttgtaa
     1201 ataccacttc atcaattatt tctttgtctt caagtaaaat atttaaatct tcagaaatga
     1261 tatcttctat gtttttgcct tgtggatttt catttatacc taaaattttt aaagcttctt
     1321 tattaaatgt gtttattata ccatctgctg aagtaaatat tattccatct tttgatagat
     1381 tcaatataac atcttgttct tctattttta aaaacagttg cctataatta tctttaactc
     1441 cattatctaa acttattaaa ctctcagaat atgccattaa atttttacgt atttctttaa
     1501 catcaattct aagaccattt attatttcta taaaagtaga tatatcaata tatctattac
     1561 caaggtcgat tactttattt atataacttg gtactttttc ctttactcca ggcgttatag
     1621 ctatgtctat atttttataa atttttcctt caatatatgg agtaaacttt aaatttttta
     1681 ctcctatttt gtaaaataag cttatcgttt ctaaggtagt ttcttttgta tcatttacaa
     1741 ccaatacttc agttccattt ggtattgaaa ataatccata tatttcattt tctttaagtg
     1801 ttctccttat cactataatc ttactatctt gtactaaatg ctttctcata ctgtttaatc
     1861 tatcatcatt cattactagt acaaaatcac cttgtatttt atcattctct tttaaaactt
     1921 ccagataata gtggtttata tcaatataat tacctaaaac caaacgtata ttttcttcta
     1981 agaaagatga tagtttagtt attttatcag ttactatagc tattgattgt ttcatattta
     2041 tacctcttat ttatatagta tataattact tatatactac tttatttcta ccaagttgtt
     2101 ttgctttata taaattttta tcagcttctt ctagtacatc tttgctatct gaaatatgag
     2161 ctacacctgc acttatagtc acagtcacat catcattcca ctttttattt gcaattgcaa
     2221 cccttatatt gtcaattata tctgctactt cctcaccaga tttatttctt attaatacag
     2281 aaaattcttc tcctccgtac ctaaagactt tatcatcttg ttctaacata acacaaatgg
     2341 tatttgttat tccctttata acagtgtcac caaatttatg tccataatta tcatttatga
     2401 gcttgaaatt atcaatatct atcatcacaa gtgcaaaatt atcatctttc tctctgtact
     2461 tttcatacat aatatcaaaa ctttttctat tataaacatt tgtaagacca tcatgtaaat
     2521 tttgtgcttt aaaatacttc aatcggttgt ataaaaaagt agaccctaat atagcaatta
     2581 aaattactgt aaataatatt ttataatatt ttatttcttt tttagttttt tcaaatagct
     2641 tactttcgtt tgatacttta tcaattatat agaaactagt ctctgaatct cttatacttt
     2701 tgttctcttt atattcattt attaacatct tttgatactc atttaaagat tctatatttc
     2761 cttgtttttc atatatattt aatatcttat ttaaattttt tattttctcc tgagttaact
     2821 ttcttttggt agatatctct aaactttttt tataattatc tattgcagaa ttataatttt
     2881 cgcttttata ttctaagttt cccattgcca tataatagta catatcttta tctaatatat
     2941 aaactgttct atctgatttt ataagataat cagctttttc taaaaaagtc tttgcctttt
     3001 gtatattatt tttgcccaca tacgcatttg acttcattat agaggccatt atatcaaaat
     3061 cattgtaatt atccgtattt acaaactttt tataatcttt tatcctgcta cttatttcaa
     3121 tagctttatc atattctccc tcttctacat atatttctgc tagatttata atagcatata
     3181 atctaaccat tccatttttt tcaccttcat caatatctat tttaaaagaa tcctttatta
     3241 atttctctcc tgtttcatag ctacctatat ttaaaaatat gctcgccaaa tcaacatcta
     3301 gtcttgcttt ttcataatta ttccccaatt tctcagccaa ttttatagac tttacagtta
     3361 aatctactgc cttagaataa ttattatcca ttatatacac tgatgctaat cttttttcta
     3421 tattaaatct acattcatca gataatttca catctgattc tctcatcttt tctaaagaat
     3481 caatgactat cttcgttcca tctggaacat atgaaatagt ctttaacata tcccaaactt
     3541 cattatatac tttattgtat tttttttctg gaatagattc gaaagaagtc ttaaccactt
     3601 tttttgcttc atcatactcc ttatctgcca caaggctttc agacatatat tttcccttat
     3661 ttataatttc tctttctttc aatccatttt ggtcatactt tataaacaaa ataatactaa
     3721 taagtattat acatataaaa attatctttt tgggtaacct tttctttggt agtctaaaat
     3781 tcaaaatttt agtcctcctt ttgtatattg tagaatgaaa acttgcatat ataaataacc
     3841 tcttaataat taataataca atatatcaaa atgatttaca acatatgcta gacttaaatc
     3901 ttaataaaaa agtctcctaa aataatttag gagactttaa atttaagcta atataaattt
     3961 atacagttaa aacaagtgct tgttttttag ttttatattt aatatttatt tatacatatt
     4021 tttttagtac tattgtagca ttatgcccac caaatcctaa agaatttgat agtgcatatc
     4081 taatattttt ttctttacct tgatttggaa cataatctaa atccaaatct tcaccaactt
     4141 cttgtatgtt tatagttgga ggcacaaaac tatcttctat actcatagca caaacaacag
     4201 cctctatagc acctgatgct cctaataagt gaccagtcat agattttgtt gatgacacac
     4261 ataagtcata tgccttttct ccaaatacag tttttatagc catagtttca tataaatcat
     4321 tgtaataagt tgaagttcca tgagcattta tataatctac ttcttctaat ggaacatttc
     4381 catctttcaa agccatattc atagacctag ctgctccctc tccattttca gctggagttg
     4441 tcatatggta tgcatcagca gttaaaccat atccaactac ttctgcatat attttagccc
     4501 ctcttgctaa agcatgttct aagtcttcta agataacaat acctgctcct tctcccatta
     4561 caaaaccatc tctgtcttta tcaaacggtc ttgatgcagt tttagggtca tcatttctag
     4621 ttgacatagc cttcatgtta cagaatccag caaaagcaag acctgttaca gcagcttcag
     4681 agccccctgc taccatgatg tctgcatcac ctctttgaat taccttaaaa gcatcaccta
     4741 ttgaatgagt tcctgatgca caagctgtac atacattagt gttaggccct tttgctccta
     4801 aaaatattga aacttgacca gctgccatat ttcctatcat cataggtaca aaaagtgaac
     4861 ttactctttt atttcctttt tccaaaagaa ttttatgttg agtttctata gcttcaactc
     4921 cacctattcc agaacctatt ataacaccaa aacgttctct atcaacttta tctaaatcaa
     4981 gatttgcatc ttcaactgct aatttagcag atactattgc aaattgagca aatttatcta
     5041 atctcttcaa ttctttttta cttatatatt cttctggatg aaaattctta acttctcctg
     5101 ctatctgagt ttgatatgtg ctagcatcaa aattagttat tttatctata ccagatactc
     5161 cagattttat attactccag aactcttctt ttcccgttcc caacggcgta acacatccaa
     5221 gtcctgttat tactactctt ttattcatcg gatacctcca cttttttaaa gctcgactat
     5281 ttattagctt ctatatactt aactatatct cctatagatt tgaagttttc tgcttctgta
     5341 tctggtattt ctataccaaa ttcatcttca agtgccatta ttatttctac tgcatctaat
     5401 gaatctgctt ctaaatcctt cattaatgat gtctccatag ttatttcttt atcttctact
     5461 cctaattgct ctattattat ttctacaact ttattaaaca ttgtgtacct cctaaaattt
     5521 tttattttaa tagatttctc tactttttaa attattattg cattaccatt ccaccatcta
     5581 catttattac ttgtcctgtt atataacttg acatgtcact aactaaaaat gctacggcat
     5641 tggctacatc ttctggttta ccataagatt tctttggtat gtttgctagt gttgattctt
     5701 ttacatcatc acttagtact ctagtcatat ctgtttctat aaatccaggt gctactgcat
     5761 ttatatttat gtttcttgat gctaactctc ttgcagaagc ttttgtaaat ccaattacac
     5821 ctgcttttga tgcacaataa ttagtttgcc ctgcattacc cataattcca actactgaag
     5881 tcatatttat tattcttcca tacttctttt tcatcatagg tttagtaact gcttttgtac
     5941 aattaaagac accttttaag tttatatcta taactctatc aaaatcttct tctttcattc
     6001 tcataagcag accatcttta gttatgcctg cattattaac caatatatca atttttccaa
     6061 attcgcttac aacagaatct atcatttgat tcacttcatc aaatttagac acatcacatt
     6121 ttatagaggt acactttacc cccatacttt ctatttcatt tttagttttt agtgcttcat
     6181 cttctttaga agtataattg attactacat cagctccaaa agatgctagt ttttttgcta
     6241 tttcttttcc tataccccta gagccaccag taacaactgc tacttgtcct gtaagattta
     6301 tcattatatc tctccatttc tataattttg tacaaattca ttaagagaat ctataccatc
     6361 tatattataa atattgacat ttgctttttt attggaagct atcttcttaa taaatgcttt
     6421 taaagttttt ccaggtccca tttctataaa agtatcatat ccatcattta aaagtatttc
     6481 tatagaatct tcccaaagaa cagatgaact tacttgtttt ataagcaggt ctttaccttc
     6541 gtttttatag taatctgcat ttacatttgc caccacatca actttaggtt tagttatgtt
     6601 aactttttct aattcactgg ccaattttac accagcttct tttaacattg atgagtgaaa
     6661 aggtccactt acatttaatt taactgccct ttttgctcct gcttctttgc attttaccat
     6721 tgcttcttct atcttttcat tttcaccaga tataactatt tgtccaggag aattgtaatt
     6781 tgctacttct acaatacctc cttgtatatc tttaaccaca ttttcaagta catttctatc
     6841 taaacctaaa attgctgcca tagctccaac acctgctgga actgtttcct gcataaactt
     6901 acctcttttt ttaactaaac atacagcatc tttaaagtca attgcatctg ctgcaaccaa
     6961 cgcactatac tctcctaagg ataatcctaa gcatgcatta tattttaaat ctatttgagc
     7021 ttgtaccgct tttaatactg ctacactatg tgtaaccata gcaggttgag tgttttcagt
     7081 tttagaaagt tcttcttcat ttccattaaa cataaggttt tttaaatcca tatttaaggc
     7141 atcactagcc tcatctatta cacttttagc aacagcatta ttttcatata aatccttggc
     7201 catacctaca tattgagctc cttgaccagg gaatactagt gcaacttttc ccatcttgct
     7261 cctcctgatt tacagttcta ttgctggcat aatctctctt gcttgattta ccatagcctc
     7321 tataatttcc ttacaaggtg ttatttcttt taccatagca gcactttgac ctgccataaa
     7381 tgaacctttt tctatgtctc catctactgt tgcaaatctt aaagctcctg tacctttttt
     7441 atccaattct tcaggagtag ctccattttg ttccatcttt aaaaattctt ttgatagttt
     7501 attttttaat gttcttactg gatgaccagt acttcttcct gttacaattg catctctatc
     7561 ttttgctttt agtactaagt ttttatagtt tgaatgcaca gaacactctt cactgcaaat
     7621 aaatctagtt cctacttgaa ctgcactggc acctaatgca aatgatgctg caattcctct
     7681 accatctaca attcctccag cagctattac aggtatgttt acagcatcag caacttgtgg
     7741 aactaagacc atagtagtaa gttctcctat atgtcctcca ccttcagtac cttctgctat
     7801 tactgctgta gctcctaact tttccattct ttgtgccaaa gctattgtag gtactacagg
     7861 aataaccttt gttccagctt cctttaatct atccatatat tttgcaggat ttccagcacc
     7921 agtagtaata acttgaactt tttcttctat aatcaaatca attatatcat caacaaatgg
     7981 cgacataagc attacattta ctccaaaagg tttatctgtt aattttttac actcaacaat
     8041 ttctttcttt atagcttctt ttggtgcgtt tcctgctgct attatgccaa gtcctcctgc
     8101 attagataca gcacttgcta atgatgcagt agctacccat gccattcccc cttggataac
     8161 tgggtatttt atatttaata ttttgcaaat tttattcata caattctcct tcacttgaaa
     8221 tcataaaagt aaacttttta ttattaatca actttatttt gtgtataagt ttaattttgt
     8281 taattaaatc attttagata ttttatacta taaagtccac ttgacaactg aagcccccca
     8341 agttagacct ccaccaaagc caactaaaac tacattatca ccctttttaa ttttaccttc
     8401 tctataagct tcatctaatg ctactggtat agatgcagca gacatatttc catatttgtc
     8461 tatatttaca taaacttttt tcatatcaag ctctaatctc ttagaagaag cttgtatgat
     8521 tcttatatta gcttgatgtg gcactaagta gtctatatca gttgtattta ggtttgcttt
     8581 ttcaagagct cttaaagaag tttctgccat ctttctaact gcaaatttaa acacatcact
     8641 acctgccatt tctattgtat gtagattagc ttctacagtc tctttacttg ccttaagtct
     8701 tgaacctcca gcaggtactt ttaaacaatc ttttccttta ccatcagagc ccatatgtgt
     8761 agacagtatt ccaccctcat ttacaggacc aataacagct gctccagctc catctccaaa
     8821 caatactgct gttgttctat catcataatt taaaaactta gaacaagttt ctgctcctat
     8881 tacaagcaca tttttatagc aacctgtttc cacaaattgc tttgcaatag ttactccata
     8941 tacaaatcca gaacaagctg ctgatatatc aaatgcagta gcattcactg ctcctattgc
     9001 atcttgtact atacatgcag ttgaaggcaa agacatatca ggagtaattg tagctaatat
     9061 aatcagctct atatcctcta cgtttaactt tgcacattct atagctttta aggcagcttt
     9121 agatgctaag tcagaagttg cttcagactc tttggcaaat cttctttcct taataccagt
     9181 tctagtactt atccactcat ctgatgtatc cattattttt tcgaagtgaa aattatcata
     9241 tgattgctca ggtagataac ttcctactcc taatattcca gcctttgtat tcattatata
     9301 ttaaacctcc aaaacgagtt tattcatctt cgttattttt attttcttca ttgtatttgc
     9361 ttataaattg gttaatatct tcaacgactt ttccctttga aaattttatt ccttgattta
     9421 tagcattttt tatagcttta gcatcagaac taccatgagc ttttataact cctccattta
     9481 gacctaaaag tggagctccc ccatactcag aataatctat aaaacttttt aattttctta
     9541 aatcatcctt tataagtaat gctcctattt tactttttgt actggccata aaagtttcct
     9601 ttataagact cataactgaa agtgctacac cttcagcaga tttaagtaat atattacctg
     9661 taaatccatc acatatgatt atatctgtgt atgcatttat aacttctcta gcttctacat
     9721 tccctataaa atttaaatct aattttttaa tttcttcata tgaccttttg actaaatcat
     9781 tgcctttgcc ttcttctagt ccaacatttg ctaatgctac cttaggattt tcaagaccta
     9841 aaacttttct tgaataaata ttactcatag ctgcaaattc aactagattc ttaggtttac
     9901 aatctgcatt tgcgccacag tctgctataa gtgtcattcc tcgctttaca tttggtattg
     9961 ctgaacataa gcaaggtcta tcgattcctt ttattctacc aacaacaaat aatccaccag
    10021 caagtaaggc tcctgtattt ccagctgata ttatagcatc tgcctttcct tcttttacaa
    10081 gatttaatgc tactaccata gatgaatctt ttttacttct tatagccttt actggtttat
    10141 cttcgttttc tataatctca gtagtatgaa ctatctctaa tttatttcta tcaaattcat
    10201 aatttgaaaa ttccttttca agtaaatctt tatctcctgt gattattaaa tcaacctgat
    10261 attcttttat agcatttact gcgccttcta cattagattt aggtgcatta tctccaccca
    10321 ttccatcaat tactattttc ataatttccc tcctaatcta attcttcaaa gataaacttt
    10381 cctctaaata tttgctcttg ggctttattg ttgataacaa cttttacata atgcttttta
    10441 tctatatttc tatacacttc tgcttttgct ataagtctat cttgttgttc tatcaactta
    10501 aaacttttta catttgcaac tctcataata accttaggtg catctattat agccattgcc
    10561 aatgaatctg ctagagaaaa aatatacgta tcttttaaag tattagtctt agagtaaatc
    10621 atgtcctgtg taacttctaa catagatata cctaatcgcc ctacagataa atcaatgatt
    10681 tctcctgtaa tttcctttac tcctaaagtc tttacttttg aactctgagt ttctgctatt
    10741 gatttgactc tttctcttag ctctggtatg tttagactca ttctatctaa tcttatggtc
    10801 tgtatactta catcaaacag acttgataat tcttcatcag tataaaaagg atctgtcttt
    10861 agcatatcta taagctcttt ttgtctttgg gctttacttt tctttttcat gttccctcca
    10921 aattttatag ctgtaattag taccagctac taatagtagt atatagtaac acacaaaaat
    10981 tttcaatact tatattatta tttttctatt ttttaatatg cataaaatcc acttattttc
    11041 tctagtaaat catcttattt ttttatatgt acaaaaaaaa catgtagagt atactctaca
    11101 tgctttttag aagctattac ttagacacaa cttctttacc tttataatat ccacagtctg
    11161 gacacactct atgtggtaat tttggctcat gacattgtgg gcaacttaca aatcctgttg
    11221 cttccatctt tgagttagct gctcttctca tttttgtgtt tgatttagac gttttacgct
    11281 ttggtactgc catttacgcc acctccttag tcaaaattta attaaataaa tcttttaatt
    11341 tagcaaaacg aggatctatg tattcctcat cgtttgtaat ttcactgcaa gaacactctt
    11401 cttcatttaa gtttgccccg catacttgac aaagaccttt acagttttca ctacaaagaa
    11461 ctttatgagg tacattaaaa tctaatgtct gttctattat atcaataagg tcgatttctt
    11521 caccatcgaa tataaaagga tcaaattcct caaactcatc ttcatcataa tcttcttctt
    11581 ttactaaaaa accttgtata gaatactcta gtggtatttc tacttcttta agacatcttg
    11641 aacaagtgtc aacaattgta aaattgacat ctatatctag atacaaacct ttattagtct
    11701 ttgacacttt cccttttaaa tttacaggtg atactaattt gtaaatctca tcacaataac
    11761 taatagtatc aattttttca caaaaattca agtctatttt gtcagtttct tttctattta
    11821 ttttttctat gctaactttc attaagttca cctcaacata tcaccaaatt tattatacaa
    11881 atctagcatt tgtttgtcaa gtatttttac ttgataggca taggtttaat ttaattattt
    11941 taccaatctt aatgtatctc tagctatcat taattcttca ttagttggta ttaaaagcat
    12001 ttttacttta gaatcagcag tagatattac tgtttctttt cctctaacat cattagcttc
    12061 tttatctaat ttcattccta aaaactccat gttagaagca atatctgctc ttatagcctt
    12121 tccattttca ccaacgcctg cagtaaatac tacaacatcc aatccattca tttcagcagc
    12181 ataagcgcct atatattttt gtactttttt aacatacgct tctaatgtag cctgagctct
    12241 ttcgtctcca tttttagctg catcttctat atctctgaag tcactagaaa ttccagtcat
    12301 tccatatact ccagattctt tattcattaa tttatccaat ccatcagcat ctaaaccttc
    12361 ttttctcatt aagaatggta atatagctgg gtctatatct ccacatctag ttcccattat
    12421 taatccttct aatggagtga atcccataga tgtatctaca cattttccac catcaacagc
    12481 agctatagaa gcaccatttc ctaagtgaca agttactatt tttaaatctt ttatatcttt
    12541 tcctaatatc tctgctgctc tctgagatac ataattatga gaagttccgt ggaatccata
    12601 tcttcttacc ccatatttag tatatagctc atgaggtaaa ccatataagt atgaagattt
    12661 aggcattgtt tgatggaaag cagtatcaaa tacacctacc attggtacat ctggaagtat
    12721 agctttacaa gcatctattc ccattatgtt tgctggatta tgaagtggag ctaaatctat
    12781 acattctttt aaagcttctt ctacttctgg agttattact actgaagttg caaatttttc
    12841 tccagcatgt acaactctat gtcctactgc atctatttct ttcatttctt taactgcacc
    12901 aaattctgga tgagcaacag cttctaaaac taatgctatt gcatctttgt ggtccttcat
    12961 tggttgttca acaacgtatt tatcatctct acctgctttt tcatgtttta atatagaacc
    13021 ttctattcct attctttcaa ccaatcctat acataaaact tcttcattat tcatatctat
    13081 taattgatat tttaatgaag aactcccaca g
//
LOCUS       DAEPOZ010000110        12977 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.110, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000110 DAEPOZ010000000
VERSION     DAEPOZ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12977
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.110"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(149..958)
                     /locus_tag="KR440_002227"
     CDS             complement(149..958)
                     /locus_tag="KR440_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0699273.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTFVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQEFLQFLEE"
     gene            complement(1042..1575)
                     /locus_tag="KR440_002228"
     CDS             complement(1042..1575)
                     /locus_tag="KR440_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBH0699274.1"
                     /translation="MSDAHYDMHKRNGYSSDEPYPEIKVLGLNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            complement(1702..3777)
                     /locus_tag="KR440_002229"
     CDS             complement(1702..3777)
                     /locus_tag="KR440_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0699275.1"
                     /translation="MLKGLSFRNARRQAGKYLIYWCSLIGSVALIYAFNALIFSDVMQ
                     ELSSILNNGGSDEFSYMIIIFSFIITFILGWFVSYMMNFILKKRSREISTYMILGVDK
                     KQIVKMLFIENLLLGASALIVGWILGIFVSKVLESIVINMFHGKYSLSPDFSLKAAGM
                     TFLYFCIIYLIAQLKSNKKLVKMKLINLLNYDRYNENVLIHQTKTKSIMFCVSVLCGG
                     MGIFFFSIPSGRLTDVFLGLLLIVFCLIVLFMGFGSIAQNMFEKGMNWKYKKSHLFLY
                     RLLASKINGISIIMGIIATLFTVSIACIGIANSFYSVMNKSVDLQQFDVSIFHPNESY
                     DFSSYEGYLSETKGVKKDYSYTLYTEEKTSFMKVRNNILSKYLKDIGRDLQYKDYTFA
                     ENQFDTFMKYSDYNILRNMLGLNPIKMDKNQFIIHCMPYLADAYKNYIGEKYKLTISD
                     KDLSIAGIYIENFSQYGGYGNGQEYIIVIPDYLIDNMKPLYSLYVAITSEPLDSAYFT
                     EFQKKFDTLENLNSGVVVAGNQGFVSKLRYDNKDYISGKYAMQSTGQAIILILPLFYL
                     ALIICIIGTVILSIQLLSENNIIIKHYGMLRTLGMIDSEVIKILKKHVCLYFSIPLIP
                     AVIFGGILVSVFSYNMFNISFDAPVFFNIQIPILHAVAITLINFVVIYCIYVFITYVA
                     LRKEILHIK"
     gene            complement(3780..4526)
                     /locus_tag="KR440_002230"
     CDS             complement(3780..4526)
                     /locus_tag="KR440_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0699276.1"
                     /translation="MLLRVENIEKYYGNQKILTKALNDVSFQINTGEFVSIMGTSGSG
                     KTTLLNCIATIDTVSAGKIYLENQDITELSEENLTVFRRDNLGFVFQDSNLLDTLTLE
                     ENIALPLTIKKVSPVEITNSVYNIATQLKIEEILSKFPYEVSGGQRQRCAVARAVVTE
                     PKLILADEPTGALDSNSSKTLMELLETINTILEATILLVTHDAISASYTERILFLRDG
                     KIFNELRKGEKSRLTFYHEILDALSLMGGD"
     gene            complement(4540..4935)
                     /locus_tag="KR440_002231"
     CDS             complement(4540..4935)
                     /locus_tag="KR440_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HBH0699277.1"
                     /translation="MKKRILTILCVVLVLFISITGLDSMGFFSRSIIPLRSEYASFIR
                     KNGTITEVKFTGPFNHNNIDLNKNVEDTNKNGDDNELTIKYDIEVKKSLISSNEPTIQ
                     IQDSKESIYVYILNFSDKTIIIKNGKIVD"
     gene            complement(5048..6070)
                     /locus_tag="KR440_002232"
     CDS             complement(5048..6070)
                     /locus_tag="KR440_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0699278.1"
                     /translation="MRFCNFLQDKVLVILLNICCAISLVLYLHLIGIGQGELTLLIAA
                     WVAILFCWICFCYQKQKKEIIKLRRIVDELDKKYLFIEMIEKPTSNIEREYYNIIRLS
                     LKSMTDHVSQIHREMYEYRQFIEQWVHEIKHPVTTSELICENYKNEHTRKIRFQMEEI
                     ERHIERALYYARLGHVEKDYLISEITLKSIVEEVLAKNKLLLIQNNIHINIQDLEYSV
                     YTDKKWIVFILNQIIINSVQYKSDSAEISFFAIKEDKSVKFTIKDNGIGIKESEIYRI
                     FELGFTGSNGRTTNNSTGIGLYLCYELSKKMGIDIDVSSSLGEYTAITLNFPINTAIN
                     MQKQCN"
     gene            complement(6067..6738)
                     /locus_tag="KR440_002233"
     CDS             complement(6067..6738)
                     /locus_tag="KR440_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0699279.1"
                     /translation="MILTNILIIEDDVFICKELMQFFENNGYSTQTLDTNNCDVLQVI
                     QESTPDLILLDINLPNIDGFSLCRQIRNCSTIPIIFITARDSAIDELNGLTMGGDDYI
                     TKPYNLPLLLARVKNLLKRVDSSDKQKIKYREIILNLISATVEYQNAVIELSRTELKI
                     MYYLFQNVGSIVPRIDLVEYLWDNHIHIDDNALSVNITRLREKLRGIGITDLIITKRG
                     MGYRI"
     gene            complement(6824..8173)
                     /locus_tag="KR440_002234"
     CDS             complement(6824..8173)
                     /locus_tag="KR440_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBH0699280.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNRANSIEEIKPCEISKLGVDNALSKV
                     GGKSIPSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIDKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEVIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            complement(8185..9567)
                     /locus_tag="KR440_002235"
     CDS             complement(8185..9567)
                     /locus_tag="KR440_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBH0699281.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNRDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDRPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            9811..11025
                     /locus_tag="KR440_002236"
     CDS             9811..11025
                     /locus_tag="KR440_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBH0699282.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIKLYGNKCDYAKRDTLLYTANKLEIKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            11202..>12977
                     /locus_tag="KR440_002237"
     CDS             11202..>12977
                     /locus_tag="KR440_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0699283.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDNIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKVSNEYTNNIVDKDNSDFECSNLD"
BASE COUNT         4640 a         2117 c         1623 g         4597 t
ORIGIN      
        1 acaaatttta ggaatatata ataatagggt ctcagaactt agttacaagt tctaaaaccc
       61 tatttgttta gtgtaaaaaa catcataaaa tttagcttaa atcttttcac ataatattac
      121 tctttttcta agtgataata aaatatatct attcctctaa aaactgtaaa aattcctgat
      181 taaatagttt aagctcatca tagaatatag catgaccact atactcaaaa gtatataaaa
      241 ttgaatcttc tattttttca ttcataaaaa ctgcaaattc atacggacat atttcatcta
      301 atttcccatg aaatatacca gtaggcactc ttacacattt taaatcatta aataattctt
      361 catctcttaa agatacagca gttccaatag tgccaatacc tgatgcacta aaaccaatat
      421 cattaaacca tcttctaaag ctttctggtg gatttgatgc aaatactttt tctccaaaat
      481 cggttaccat ttctggtcta tcagtacatg cttgtgctat caatttattt acatcttcac
      541 gtgtcatacc atatggaaat tctggaggtc tttgtacaaa tgatggtgca gcagctgcag
      601 ctaataccaa ttttgacact ccatatccat taaataaact catataccta agtacgattg
      661 ctcctcccat tgaaaaacca acaaaggtaa agtcttttaa tccaatagca tgtaccacct
      721 tataaatatc gtctgctaat tggctataag tatatcctcc tgatgttgca tcagatttac
      781 caaaacctct taaatcaatt gaaactgttc tatatccgag ttttggcaat atatttgttt
      841 gatattcaaa tatcttatgt cctaagggcc aaccatgtat aaaaagtaca gtttttttag
      901 cactcggatt caaatcatat actgctatgt ttatatcatt cacatataca taatacatat
      961 caacagcaac tcccttcaag aaaatgtatg atggttattt tatatatatg aataaaatga
     1021 tgaataacta aatttttaaa tttatttaca atacttctct attgcctttt caaataaact
     1081 tacatgaagt ttttcatcta ggattattct atttaatata gcttttatat aagggtcttc
     1141 aataagactt atatctttat aataattatt aatggccaca tgctccaaat gcaaatctag
     1201 ttttaatctg ttacaaatag aatttccata acatacaaaa ccaccattcc aatatgcacc
     1261 acaagaactt gtagaacctc tatatacagg atttccaccc aataatctta tagtctttgc
     1321 taatatctcc atatgtaaca tttctgttat agatatattt atccacattt cacttaactc
     1381 tctatgcgtt tcatctaaat caaaattatg gtatagatat tgatttacac tcgtaaattc
     1441 acttgatact ccggcatagt catccatcaa taattctgca taatacttat ttagacctaa
     1501 aacttttatc tctggatatg gctcatcact tgaatatcca tttcgcttgt gcatgtcgta
     1561 atgtgcatca ctcataatat ctcctccttt aaatttaata cttgatatta attgtatgca
     1621 tcacgacttt ttcatataac ttagataata aaatctatta tattattttc tattataatt
     1681 tatgtgtcaa ttcacccaaa tttatttgat atgcaatatt tctttcctta gtgctacata
     1741 tgtaataaaa acatatatac aatatattac tacaaaatta ataagcgtaa tggctacagc
     1801 atgtaagata ggaatctgaa tattaaaaaa tactggagca tcaaaagaaa tattaaacat
     1861 gttgtatgaa aaaactgata ccaatattcc accaaatatt actgcaggaa ttaatggaat
     1921 cgaaaaatat aaacaaacat gtttctttaa gatttttatt acttcactat caatcatccc
     1981 caaagttcga agcattccat agtgttttat gataatgtta ttttcgctca ataattgaat
     2041 agagagaatg actgtaccaa taatgcatat aatgagtgcc aaataaaaaa gtggtaatat
     2101 caagataatg gcttgaccag tagattgcat agcatacttt ccacttatat aatccttatt
     2161 atcatatcgt aatttagaaa caaagccttg attacctgct actactactc cagaattaag
     2221 attttctaaa gtatcaaact ttttctgaaa ctcagtaaaa taagcactat ccaaaggctc
     2281 acttgtaata gcaacataaa gactatacag aggtttcata ttatcaatta aataatctgg
     2341 aatgacaata atatattctt gtccatttcc atatcctcca tactgtgaaa agttctctat
     2401 ataaatacct gcaatactta agtccttatc tgatattgtc agtttatatt tttctccaat
     2461 ataattctta tatgcatcag caagataagg catacaatgg atgataaatt gatttttgtc
     2521 catctttatt gggtttaaac ccaacatatt tcttagtata ttgtaatcac tatatttcat
     2581 aaatgtgtca aactggtttt ctgcaaaagt ataatcctta tattgtaaat ctcgccctat
     2641 atctttcaag tatttagaaa ggatattatt acgaaccttc ataaaggatg ttttttcttc
     2701 tgtgtaaaga gtataagaat aatccttttt cacacctttc gtttctgata aatatccttc
     2761 atatgaagaa aaatcatatg attcattcgg gtgaaaaata ctcacatcaa attgttgtaa
     2821 atccactgat ttattcatca cagaataaaa tgagttagct attccaatac aagcaattga
     2881 tactgtaaaa agagttgcga taatacccat tataatgctt attccattta tctttgaagc
     2941 cagtagacga tacaaaaata agtgagattt cttatatttc caattcatcc ctttttcaaa
     3001 catattctgt gcaatagatc caaatcccat aaataatacg attagacaaa atacaatcaa
     3061 caataaacca agaaatacat ctgttaatct accactaggt attgaaaaga aaaaaatacc
     3121 catacctcca cacaaaacag acacacaaaa cataatagac ttcgtttttg tttggtgaat
     3181 taatacattt tcattatatc gatcataatt aagcaaatta attaatttca ttttaacaag
     3241 ttttttatta ctttttaatt gagcaattaa atatattatg cagaagtata aaaaagtcat
     3301 cccagcagct tttaatgaaa aatccggaga taaagaatac tttccatgaa acatattaat
     3361 aacaatagat tccaaaacct tagaaacaaa tatccctaga atccatccaa caataagagc
     3421 agaagcacct aataaaagat tctctataaa aagcatttta acaatttgtt ttttatctac
     3481 tcctaatatc atataagtac ttatttctct gcttcttttt ttcaatataa aattcatcat
     3541 ataactaaca aaccagccta aaataaaagt aataatgaac gaaaatatga taatcatata
     3601 agaaaactca tcgcttcctc cattattaag tatactagaa agttcttgca ttacgtcgga
     3661 aaatatcaaa gcgttaaaag cataaatcaa agctacagag cctattaagg aacaccaata
     3721 aataaggtac tttcctgctt gtcgtcttgc attacgaaaa gataatcctt tcaacataat
     3781 caatctcctc ccatcaaaga aagtgcatcc aaaatttcgt gatagaaagt aagtctggat
     3841 ttttctcctt ttctaagttc gttaaaaatt ttcccatccc ttagaaataa aattctctca
     3901 gtataactag ctgaaatagc gtcgtgtgtg acaagaagaa tcgtagcctc taatatggtg
     3961 tttatagttt ccaaaagttc cattaaagtt ttagatgaat tagaatccaa tgcaccagtt
     4021 ggttcatctg ccaaaataag tttgggctct gttacaactg cacgagctac tgcacaacgt
     4081 tgcctctgac caccagatac ttcataagga aacttcgaaa gtatctcttc aattttcaat
     4141 tgtgtggcaa tattatacac actattagta atttcaacag gagaaacttt ttttattgta
     4201 agtggaagtg ctatattttc ttctaatgtc agagtatcta ataaattaga atcctgaaaa
     4261 acaaaaccta aattatcacg acgaaaaaca gttaagtttt cttcggatag ttcagtaata
     4321 tcttgatttt caagataaat ttttccagca cttactgtat caattgtagc aatacaattt
     4381 aacaaagtag ttttaccaga gccagaagtt cccataatac tgacaaactc tcctgtatta
     4441 atttgaaatg acacatcatt caaggccttt gtaagtattt tctgattgcc ataatatttc
     4501 tcaatgttct ctactcgtaa tagcatgtta aagtcctcct tagtcaacaa tttttccatt
     4561 tttaattatt attgtcttat ctgaaaaatt caagatatat acatatatgc tttctttaga
     4621 gtcttgaatt tgaatagtag gttcattaga tgaaattaga gattttttta cttcaatatc
     4681 gtattttatt gttaattcat tatcatctcc atttttatta gtatcttcta catttttatt
     4741 taaatcaatg ttattatgat taaagggacc agtaaacttt acttctgtaa tagttccatt
     4801 ttttctaata aaagaagcat attcagacct tagtggtatg atacttcgtg agaaaaaacc
     4861 catcgaatcc aatcctgtaa ttgaaataaa caaaacgagt acaacacata atatagttaa
     4921 tatccttttt ttcatatgtt tctccctcct ttctattgat attgtagcgt aaatagtaat
     4981 ctgaatatat agaagttact taactttatc ttacagtttt gaaagataaa gaaatagcag
     5041 cgcattttta gttgcactgc ttctgcatat tgatagctgt atttattggg aaattcaatg
     5101 ttattgctgt gtattcacca aggctggagg acacatctat atcaattccc attttcttag
     5161 acagttcata acaaaggtaa agaccaatcc ctgtagaatt gtttgtagtc cttccattac
     5221 tgccagtaaa acctaattca aaaattcgat aaatttcact ttctttgatg ccaataccat
     5281 tatcttttat agtaaactta acactcttgt cttcttttat tgcaaaaaat gaaatttcag
     5341 ctgaatcact tttgtactga acactattga taataatttg atttaaaata aaaactatcc
     5401 actttttatc agtatataca ctatactcca aatcctgtat attaatatga atattatttt
     5461 gtataagaag caatttattc tttgctaata cttcttctac aattgacttt aatgttattt
     5521 cagaaattaa ataatctttt tcaacatggc caaggcgagc atagtacaat gcacgctcaa
     5581 tatgacgttc tatttcttcc atttgaaaac gaatttttcg tgtatgttca tttttataat
     5641 tttcacatat aagttctgat gttgtaacag gatgttttat ctcatgtacc cattgttcaa
     5701 tgaattgtct atattcgtac atttcacgat gtatttgaga aacatgatct gtcatagatt
     5761 tcaatgataa tctaataata ttgtagtatt cacgttctat gttagaagta ggtttttcaa
     5821 tcatttcaat aaacagatat tttttatcca attcgtctac aatgcgacgt aacttaataa
     5881 tttctttttt ctgtttttga taacaaaaac atatccaaca aaataaaatt gcgacccaag
     5941 cggctataag aagagtcaac tctccttgac caatccctat aagatgaaga tatagcacta
     6001 atgaaattgc acaacaaata tttaagagta taactaatac tttatcttga agaaaattac
     6061 aaaacctcat atcctatatc ccatcccccg ttttgtaatg attaagtcag taatacctat
     6121 accccttaac ttttctctaa gccttgtaat gtttacactt aaagcattat catcaatatg
     6181 aatatgattg tcccataaat attctaccaa atctatacga ggcacaatac tccctacatt
     6241 ctgaaacaaa taatacatga tttttagttc agttcttgaa agttctataa cagcattttg
     6301 gtattctact gtagctgaaa taaggttgag tataatctct ctatatttaa ttttctgttt
     6361 atctgatgaa tctactcttt taagtaggtt ctttaccctt gctaaaagca aaggtaagtt
     6421 atatggtttt gtaatatagt catctccccc catagtcaaa ccatttagtt catcaattgc
     6481 actatctctt gctgtaataa aaataatagg tatagtagaa caattcctaa tctgtctaca
     6541 taaagaaaaa ccatctatat taggcaaatt gatatccaag agaattaagt caggtgttga
     6601 ttcttggatt acttgaagta catcacaatt atttgtatcc aatgtttgtg tactatagcc
     6661 attattttca aagaattgca ttaattcctt acagatgaac acatcatctt caattatcaa
     6721 aatattggtt aaaataataa atacctcatt tctttagttt atattgcttt aattttgtaa
     6781 gtaatatatt tttccatata ttacttacaa aattactttt taattattca tcttttccag
     6841 ctactgatag ctctttaata acaactgaag gtgaacctat actactcatt ggaaattcta
     6901 aatcttcccc tataacttca atatctttca acaggtcaaa atagtttcct gctactgtaa
     6961 tttgctccac tggaaatact ttttttcctt ctttgatata aaagcctttg gcagctaaag
     7021 aaaagtctcc tgttactgaa tttgcacctg aatgaagccc tgcaaagcta gtaaccatca
     7081 atccttcatc tatatccttt ataatttcat ctaaagattt atctccttta tctatataaa
     7141 aattagttgg atatatgctt ataggtgatg aatatgaaga ttttattcca tttcctgtag
     7201 tctttacacc tgccttatta gccgttttta agttatgaag taaagttata agctttccat
     7261 ttaaaacaac ttctttttta aatgtagcta ctccttcatc atcaaaagga gtagatgcca
     7321 taccattttc taaaagtggg tcatcaacta ttgtaactat aggagatgct atcatgtctc
     7381 cttctctatt tttcaataaa gataatcctt tttgagctga atctgcatta aatatcccag
     7441 aaaatgtgct aagcaaagat accatagctt cattaagcaa tatagtttta tatttacctg
     7501 atggaatact cttaccacct actttagata aagcattatc aacaccaagt tttgatatct
     7561 cacaaggttt tatttcttca atagaatttg ctctattata tcctattcca tcatattttt
     7621 gtccattgtc ttctatgata ggaactacaa accctataag catattagtt ttattgctta
     7681 aattaagacc tttagtatta gatataccat tacttgatac agaataagat atagtacatt
     7741 tagataaatt taccacctta tctgaataag ctttagtctc tctttccatt tctaaagcta
     7801 aatcaatcaa cttatctgcc tctaaatttt ctagctcttt agaataagtc tttacatcat
     7861 tatagtgctt atctccttca tatataaatt gtacatcttc attttcaata gtgtttactc
     7921 catcttttac attttttatc aacatatcaa cagctttatc atctaaaatt tctgtataag
     7981 aataacctat ctttccattt accttacctc taaaagataa accaaaagat ttatcaagat
     8041 tgtatttctc aacttctcct tcatatatat ttatacttaa attttctcct gtataataat
     8101 atatttcaca ctcttcaaaa ccttcactta gagccttttt aaataatata tctttaaaat
     8161 ttttaagttc cacaattatc cctcctatct tccacctaca gtaatttctt ttacccttat
     8221 cataggttgg cctacattag ttggtatact tcctgatgat gagccacaca tcccctgagc
     8281 ttgttttacg ttattcccaa ccatatctat attcataagt atatcgctac cttttccaat
     8341 taaactagca cctctcactg gctcttgtat ttctccattt tttattaagt aaccttctga
     8401 aactgcaaaa ttaaactctc ctgttacagg atttacagaa ccaccaccca tttttttagc
     8461 ataaagtcca tctggtattg actttataat gtcttctggt ctatcttctc cagcagcaat
     8521 ataagtgtta gtcatccttg aagttggtgc aaatttgtag ttctgtctac gagaacttcc
     8581 tgtaggattc attcccattc gtctaccatt aagtttatct atcatataag acttcaaaat
     8641 tccattttca atcagtatat tcttttgagt tggatttcct tcatcatcta tattcataga
     8701 accccagtag tttggtattg taccatcatc tatagctgta atctttgtgg aagcaatctg
     8761 ttgacctaat ttatctgcaa atacagagtt cccctttgca actgatgtag cttccaaaga
     8821 atgtccacaa gcttcatgaa atattactcc accaaatcca ttatctattg ctaccatcat
     8881 attacctgca ggacaatttt tagcatgcaa catagtatgc gctactctag ctgattcttt
     8941 tgcataatat tctgggtcta tttcttcaaa tatttcaaac cctttacaac ctccaggacc
     9001 ttcaaatcca gtttgatttt cattaccctt agatgcaatt gaacttatcc ctagtctggt
     9061 tcttattctt ctatcctcaa catatatacc ttcagtgttt gcaattaata ttttttggtc
     9121 tttatccaaa tagctaacac ttacttgaga tatatcatta ctaaattctt ttgcactctt
     9181 atatgcaacc ttcataacac ttactttatc tctatttcct attgaatttg gataaatttt
     9241 tatattatgt atgttattta ttttaatact gtcatttaat attacacttt ttccatcttc
     9301 caatttacct aaagcaacag cagctttgta tgctacatcc aacagactat ttaatgaatt
     9361 gtcacttgta taagcatata tactcttcaa tcctttaaat attcttattc ctataccata
     9421 atttcttcca cctattgcat tttctacttt attatcaatt aaactaatag aattatttat
     9481 agtgtcttct tcaaaaatct cagcaaaatc tcctccagta attagacact tttctaatac
     9541 cttagaagct atattttttg aaagcataaa tttacctcct actttaaatt tttaattaac
     9601 ttttattcaa tctttttact tgttaacaaa cattatatac tatattactt aaaataaggt
     9661 aattatgaat atattataca tatgttttat actaaaatgt tatcacattt tatctataca
     9721 tacccatata actagataat taaaatgtat aatattattt gctatagaaa ataatacact
     9781 catacataat aaattttagg agtgaagtat atgaatcaag aatatgtaaa aggaaatcct
     9841 atttttacta atattaataa agttccaaga caatttccat accttactga tgatattgat
     9901 actgatgtta taattgttgg tggtggcgta actggatgta tttgtgctta ctatctagct
     9961 aaaaacaata taaagtctgt cattcttgaa aaaggaagga ttgcacatgg aagcactagc
    10021 gtgacaactt ctctattaca gtatgaatta gatgacaatt tgatagattt aacagaggtt
    10081 atgactctaa aagatgctct aaaagcatat aacctttgta cttcagcact tgaagaatta
    10141 gatacattta taaagctata tggaaataag tgtgattatg ccaaaagaga tactctactt
    10201 tatactgcca acaaacttga aattaaggct atcaaagaag aatataactt aagaaaagaa
    10261 aatggatttg atgttgaata tattgatgaa tccacaaacc cttttagctt tgatttaaaa
    10321 tcaggattga tagcaaagaa tggaggacga gaattagacc catataaata tagtcaccat
    10381 ttgattgatg taagtttaaa aaatggtctt caagtatatg aaaatacaga agtaaaaaaa
    10441 gtagatttat caaatgataa ggttactgct gaagtatcgt atggatacaa agttcatggt
    10501 aaaaaattaa ttgtagctac agggtacaat acaagtttat ttacaaaaag aaattttgca
    10561 actaagtcaa atacttttaa tatagcaact aaaccactta aaaacatagc ttcttggaaa
    10621 aacaacattt taattagaga taactgtgac ccatataatt atctaagaac tactaaagac
    10681 aatcgattaa ttataggtgg tgaagatgta agttttgatg atatagaaaa tgaaacttta
    10741 gccaatgaaa agtatgatat tttagaacaa agattgaaaa gtatgtttaa ggatataaaa
    10801 gacattgaag ttgaatataa atattgtggt tgttttgctt caactttaga taatttaggt
    10861 tttattggtc cagataataa aaataacaat ctatggtatt gcttaggtta tggagcaaat
    10921 ggtatactct ttgcaatatt aggaggtata atgttaagtg aattatatct tggcaaacaa
    10981 aataaaaata tgaaattatt taaagtagat agatttgata aatagatatt gtatattaaa
    11041 agactataaa aagatgaaac tttataattc taaaagttaa ctttgataca gtcttttata
    11101 atgttccttt taaaaatact ttatttataa ttaaatataa agtaaaatgt taaaatataa
    11161 ctgataaaat ttagtcataa gataatattt aagtttattt gatgaaatat ggaggaatta
    11221 ttatgaaaga aatactcgtt attgctccca ctaaaggaac ctatgaaaaa tcaattcaca
    11281 tagttaaaaa aaacaaatat acaaatattg atgttgtatt tggcaatcta aaagaaggta
    11341 tacctcttgc tgaaaaaagt attaatcatg gtactagaat tatcataagc agaggtggaa
    11401 cttacaatat gttaaaagct acttacaata tacctatagt tgaaattaaa gtagatgctt
    11461 atgatattat aaaaagttac aaagaggtaa aaaattccaa cgaaccattt ggaataattg
    11521 gatttaataa tgtcatctac ggtttcgata ttatagaaga aatattgaat aagaaaataa
    11581 ctatgattga aattgaaaaa gaagaagaaa tttatgatgc tatagaaaag tacagaaaga
    11641 aaggtataaa cacatatata ggagatacca ctgttgcaca tattgttaag agacttaatt
    11701 gtaagggaat attaatcgaa tctagagaag aaaacatact tagagccatt caacaagcag
    11761 aacaaatact cgaagcaacc aaggatgaac aaaaaagaag attacagata gaagctatga
    11821 cagattttgt acatgatgga ataataacag ttgataaaaa ttttaaaata acattattta
    11881 ataagagtgc tgaaaaaata tttggtataa aaaaagaaaa tgcactttat cataatgttg
    11941 tagatgttat cccaaacaca gttcttccac aagtagttga gactatggaa cctcaaattg
    12001 gtgaaataca aaatataggg agtacaaaaa ttattacaaa tagagttcca ataattgttg
    12061 atggccagat tcaaggggct gtttcaacat ttcaagatag caaggagata agttcttttg
    12121 aacatacaat aagaaggagc ttattaagca aaggtctatc tgctaaatac acttttgata
    12181 atataattca tgctagtgaa aagacaaaag attgtataga acttgcaaaa aaatatgctt
    12241 gttatgatac tcctatgatt attactggag aatctggagt tggtaaagaa ttattttgtc
    12301 aaagtataca taactatagt aagcgtaaaa atgcaccttt tgttgctgta aattgtgctg
    12361 ccatacctcc atctctaata gagagtgagt tttttggtta tgaggaaggt tcatttacag
    12421 gtgctagaaa aaaaggtaaa gcaggtgtat tcgaactagc acatgaaggt acaatcttcc
    12481 tagacgaaat aagtgaaatc cctcttgaac ttcaaggtcg attattgagg gtattgcagg
    12541 aaaaacaagt tatgagaatt ggtggtgata aagttattcc tatagatgta aaaattatct
    12601 gtgcttcaaa taaagactta aaaaatatga tgcgcaatgg tttatttaga caagaccttt
    12661 attttagaat aaatatacta acattatatc ttccttcatt aaaagaaaga aaagaagata
    12721 ttataaaatt atcagaattt tttatacata aatattcaag caaatacaat aaaacgcctt
    12781 taattatcac tccaaatatt aaaaaggcac ttcttgaacg agaatatgaa ggtaatataa
    12841 gggaattgga aggtatgatg gaaagggctg ttattgtgga atcatttgac tcgattttat
    12901 tagaaaaggt tagcaatgaa tatacaaata atatagttga taaagataat agtgattttg
    12961 aatgcagtaa tttagat
//
LOCUS       DAEPOZ010000111        12971 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.111, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000111 DAEPOZ010000000
VERSION     DAEPOZ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12971
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.111"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            2..883
                     /locus_tag="KR440_002238"
     CDS             2..883
                     /locus_tag="KR440_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0699284.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            complement(1091..1954)
                     /locus_tag="KR440_002239"
     CDS             complement(1091..1954)
                     /locus_tag="KR440_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HBH0699285.1"
                     /translation="MLSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIV
                     EPVGPWLEKMR"
     gene            complement(2109..4043)
                     /locus_tag="KR440_002240"
     CDS             complement(2109..4043)
                     /locus_tag="KR440_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HBH0699286.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKAINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLSV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSLEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            complement(4155..5168)
                     /gene="galE"
                     /locus_tag="KR440_002241"
     CDS             complement(4155..5168)
                     /gene="galE"
                     /locus_tag="KR440_002241"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBH0699287.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            complement(5193..6062)
                     /gene="galU"
                     /locus_tag="KR440_002242"
     CDS             complement(5193..6062)
                     /gene="galU"
                     /locus_tag="KR440_002242"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBH0699288.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            complement(6260..6580)
                     /locus_tag="KR440_002243"
     CDS             complement(6260..6580)
                     /locus_tag="KR440_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699289.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            complement(6570..8561)
                     /locus_tag="KR440_002244"
     CDS             complement(6570..8561)
                     /locus_tag="KR440_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699290.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCDIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKNMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            complement(8683..11226)
                     /locus_tag="KR440_002245"
     CDS             complement(8683..11226)
                     /locus_tag="KR440_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HBH0699291.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQDKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESISQTIHNEYK
                     KLCGLEELHSVEGYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGAKEEVDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEVEQEEENLNDISPDIILDKPVENHQAKSEEIEQNEL
                     KEIKQEEPSQHIEEERSVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            complement(11504..11838)
                     /gene="rnpB"
                     /locus_tag="KR440_002246"
     ncRNA           complement(11504..11838)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="KR440_002246"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            11970..12647
                     /gene="srtB"
                     /locus_tag="KR440_002247"
     CDS             11970..12647
                     /gene="srtB"
                     /locus_tag="KR440_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HBH0699292.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKTSSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKNNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            complement(12807..12881)
                     /locus_tag="KR440_002248"
     tRNA            complement(12807..12881)
                     /locus_tag="KR440_002248"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12847..12849),aa:Thr,seq:tgt)
BASE COUNT         3986 a         2288 c         1321 g         5376 t
ORIGIN      
        1 attgaattta tatcacttac gctattttgt tacattggca catttagaac actatacaaa
       61 agcggcagaa aatttatcta taacacaacc aagcttaagt catgctattt ctttactaga
      121 aaatgaactt ggggtggctc tatttgaaaa agaaggtaga aatatagtcc ttactaagta
      181 tggaaaaata tttttaaaag atgtggaaaa atcattagaa attttagatt caagtgtaaa
      241 atcattaaaa ataactggta ctggtgaagg acaaatagac ttagcttttc ttagaacact
      301 tggtactgat tttatcccag acatagtgca taaattttta aaatctaatc ctgctaaatc
      361 aatagatttt aaatttcata ctggtgtaac tactgacatt attcaaggtc ttaaagaaag
      421 aaaatatgat attgcatttt gttcgaaact tgaaaaagaa aagggaattg aatttatacc
      481 tgttgccaaa caagatttag tactaattgt accatatagt catccactag ctgcaaagga
      541 tactatagat ttaaaagaaa ccatacctta tcctcaaata gtatttaatc agagaagtgg
      601 tcttagatat ataattgatg atatgtttaa aaaaataaat caacagccta atatagtcta
      661 tgaagtagag gaagaccaag taattgctgg attagttgca aaaaatttcg gaattgctgt
      721 agtcccaaat atgaatatgc ttagctttac aaaagtaaaa gtaattcaga ttattcaccc
      781 aagttgggaa agaaactttt acttagcatt tataaaagat agatatttgc ctcctgcaat
      841 taaaaacttt aaaaactttg tcattaaaaa cgctcaatta taatttatta ataaaggggt
      901 tatttttaaa tatcctcttt tattcttttt ataaaataaa aaaatctata cttttaaaag
      961 tatagattcc taactgacag tcggcatccg agtctcagta attatcgtta tacaacagca
     1021 actggattca gttcctgaat aactaggtta tttacattat atcagatttt tttaaatttg
     1081 tcaattgtat ctaacgcatt ttttcaagcc atggtccaac aggctctaca ataggaactt
     1141 ctttaaatcc ccaatttctt atatcattca cactcgttgg agtatatatt ttgtttctga
     1201 cagatttgaa tattttgttt ttttctccat attgcataca ctttggatta gcttctatta
     1261 accctcttct tgctctgcta agaggattac ataaaatatc tgctatttct aaaccttctc
     1321 tatagtcagc atcttctttt ttggcaatta taaaaccctt aaccttatct cttaattctt
     1381 catctatatc taagtatatt gttccattat taaatacatc aaaaaacgcc ttttgtactt
     1441 ttaagtttga gacatcatcc cttgactcta taactattcg tgcagattct acagtttcat
     1501 tatttacaaa tgaataaaaa ttctgaagaa catgtataaa agccacaaca tacggttcct
     1561 ttgaaggtgc ataatatttc tttaatctag ctttatctac tgttactgat attatagtac
     1621 actctaatct agatagaaat aaaggcaact catcatagaa taacttaatc tgtgaatcat
     1681 caaacttctc aaatgctctt gattttcttg atatatctgt caaatgtatt cctatgttac
     1741 tggttccaaa acaatactct ttaaatgcat ccatttcttt cctcaaagaa catacataat
     1801 cgccttgact tactgcatat ttatattcaa aaattactcc tgtcaatgta aatgggtctt
     1861 catgatttat atcagctctt ccactttcat caataaacat tatatatttt ttctttataa
     1921 tcttactcac cctcttaaca tatgatgata gcatctatat tcattttata tgagttttaa
     1981 aaatattcca agatttttat ataacttaaa cttaatttta tactaaaaaa ttgagatgta
     2041 aaacaatcaa gcttttattg attgaatttc acatctcaat tataattatt tttcaatata
     2101 tatttttact attttgcaag aatatccatt aatttatcca ttactatata agatactccg
     2161 cctcctattt gataaggagt ttcaactttt ttatttgcaa atactctttg ttgctctaat
     2221 gataatcctg aattatccat taaaactatt ggtgaatgta attttacagc taatggtcca
     2281 gctgataaag catctactaa agcatctgat ttactaacta atacagcctg taaatcagta
     2341 tctccataga atttttctat tactttggca ttagtatcta ctctactatc tccatatatt
     2401 ctatttcctg aaatatcttc agtagttata tcattcattt ttgctattac tgaatcgtta
     2461 attgcacttg gtccacctat aaagtatgca ttttctaatt ttctttctgt tagccattta
     2521 taagattctt ctgtaattcc atctttattg actaatatta taggttgttt ttcttcccca
     2581 gctttagaag ctacagataa agcatctgct tccccatttc cagaaaccat atatgcagtt
     2641 tttatttctt ctcgatttcc aagttcttca gctattcttg tagcagtttg atatctatct
     2701 tgacctgcta ttctctttgt ctctgtgttg aaagtagttt ttatttcttg ctctaattta
     2761 gaacttattg cattttctcc acctatcaag attactgttt taggtgctaa taacttcatt
     2821 tcttcaacag tttcagttgg taaattagct gtattagtaa gtagtattgt tgagtcatag
     2881 ctacttgcta aaggagttgc tgttatacca tcagctattg cattaccatt tactaatatt
     2941 acagtatctg aactatcagc attccagttt ccaactgaac ttatcttttt agctgtatca
     3001 aatctatttg cacctataat tgtatcccat gtaggattta tagttccatc ttcattaaaa
     3061 gtataatctt tcccatctat tttttgagaa ccagttagca tctttccatc tttgtctaaa
     3121 tagtaagact taccatctat ttgttgccat cctgtctgca ttattcctgt tttgtcaaag
     3181 taatacttat taccatctat ttcttgccat cctgttactg ataaaccatc agatcctaga
     3241 tagtatttct ttccattctt ttcttgccat cctgtttgca ttactccatc tgagttaaag
     3301 taataagttt ttccgtctat tactttccat ccagatactc ttgctccatc agttcctaaa
     3361 taatattttt tgttattaat tgctttccat cctgtttgca ttactcctga agcatcaaag
     3421 taatacattt ttccatttac ttttagccaa ctattctttt ggtatgttcc atcagatttt
     3481 acataatact tcttaccatt ttctgttttc catcctgttt gatttagatt tatcttatat
     3541 tttgccgcta tctttacata gctgtcatta ctataatcta taattacatc tgaacctaca
     3601 ggaacttggt caaataacca tctaacatct ttattgtgca ttcttataca tccaccagaa
     3661 atatgctttc ctatagaact ctcattatta tttccatgta ttccatatgt atctccataa
     3721 gttcctttta atgctaatcc catccatcta tctcctaatg gatttcttgg acttcctcca
     3781 ggtatattac ctttataata tggtctattt tttatcttgt taactacttt tgttttccct
     3841 gttggtgttt ctgacccctt tttacctgtt gcaactctaa attcttttac aagtttatta
     3901 tttacaaaat atcctagagt attctttctt gaatttacta taatcacatg tttactggct
     3961 gcgcttgatt ctggtacgcc acatgcaaat actaaaacta aagtcagtgc tagtactaaa
     4021 tactttttta gacttttttt cactataact tccccctctt ttagaaacat tactacatgt
     4081 gtctaattat aacataaaat taccaatacc ttatatttac aaatttgtta catttataca
     4141 tataagtatg tattctactc ttcataccca ttaggattca tactttgcca tctccatgaa
     4201 tcttcacaca tctcttcaag ttcatactta gctttccatc caagctcttt ttctgccttt
     4261 gaagaatctg cataacacat ggctacatct ccagcccttc ttcctacaat cttatatggt
     4321 atttcttttc cacttgcttt agaaaatgct tttactaaat caagaacact ataacccttt
     4381 ccagttccta aattataaac aacaagtcct ggattttctt ctaatttttg taatgctttt
     4441 acatgaccag cagctaaatc taatacatgt atatagtctc taactccagt tccatcatgt
     4501 gttggataat cattaccata aacactcaat tccttcaatt tacctactgc tatttttgtt
     4561 atataaggca ttagattgct cggaactcca tttggttctt cacctattct accactttta
     4621 tgagcaccaa ctggattaaa gtatcttaaa agagctatat ccaatgactt atctgctttt
     4681 gatatatcta ctaacatctg ctctatcata agtttagttc ttccatatgg attggttaca
     4741 gaaagtggga aatcctctaa tataggacaa gtatgtgggt ctccatatac agtagctgaa
     4801 gaactaaata caaacttttt aacaccatat tctctcatta attcaaataa atttaaggta
     4861 cttattagat tattgctgta atattcaatt ggtttttcta ctgattcacc aactgctttt
     4921 aaagctgcaa agtgtataat tgattcaata ttattttctt taaatacaac atgcatttca
     4981 tctttatttc tgatatcaat attataaaat tttactggtt ttttagataa ttccttaatt
     5041 ctatcaacca ctattgagtt gctattactt aagttatcaa ctataacaac ttcataacct
     5101 gattctaaaa gttctattgc tgtatggctt cctatatatc ctgctcctcc agctactaaa
     5161 actgccattg taattacctc ttttctaata ttttattttt cacttacttt ttttctcata
     5221 tagtccatta aatcatcttt taaattttct ctctttagag caaagtctat tgttgcctct
     5281 aaaaaaccaa tcttatctcc cacatcatat cttctacctt caaagttata agcataaata
     5341 gcctcttgtt ttccaagagt ttgaagtgca tctgtcagtt gtatctctcc acctttacca
     5401 ggtgtctgtt tctctaatat actaaatatt gcaggagtta ttatatatct tccaagtata
     5461 gctatatcag atggagcttc ttcaattgca ggtttttcaa ccatatcttt aactttatat
     5521 actctatctt cgatatgttt tacatccaat ataccatatt tattagtatc ttctttagct
     5581 atcttttgaa cacctaatac agttgtttta tactcattat aagtatctat taattgtttt
     5641 aagcacggaa catctgaatc tactatatca tcacctaata atacggcaaa aggttcatca
     5701 cctataaaac tttttgcaca gtaaattgca tgtccaagac cttttggctc tttttgtctt
     5761 atataatgaa tatttaccat atttgatatg tctcttacca tttctaacat ctctgtctta
     5821 cctttttgct ctagttctaa ctctaattca actgatttat caaaatgatc ttctatagat
     5881 tttttatttc tacctgttat tataagaatt tcttcaattc ccgattctat agcttcttct
     5941 attatatact gtaaagttgg tttatctact ataggaagca tttctttggg ctgtgatttt
     6001 gttgctggta aaaatctggt tcctagccct gctgctggta ttattgcttt tttaactgtc
     6061 atctgcattt tttcaccctt cttcgtaaat tttctattgt accaaattaa tataccatat
     6121 ttatactttt tcttcaaact ctatattatc ccaatattta atgctataag ataatataag
     6181 gtagatgaaa tttcatctac ctttagtttt ataagttaat attgtattgt ttatatttta
     6241 tccaaaactt atacaggtac taaatttcat ctaagatgcc ttttatatca ctatctatat
     6301 cttcatctag ataagaatct aaatcttcta agtcaacttc tagttcttga tatgtcatat
     6361 caacactgac atcctcatct atatccattt ctgatattaa ttctcctaaa atactatttc
     6421 catcagaatc taaaccacta tctatatctt catctatata gttttcattt aaaatttcat
     6481 ctaaatcaat ctcgtaataa acttcattat ttttttcatc ccctgagtcc aatcctaaga
     6541 actcatagtt aaaagtccaa acattgtatt tacttgacat actttattcc tcctgtgaaa
     6601 aacatatttt catctagttt attggttcca taatcaacag agttatatat tttttcaatt
     6661 atgtcttttc ttctttcttt tgtactatta tttaatgtca attgtttaaa tacatatgta
     6721 tttgttattc tctcgaataa tatttctttt atactatcat catcaatatt tctatagttt
     6781 ttccacaata actcaaataa cttaaacatt tccctactag tttcatctac ataataagga
     6841 tacatatttt ctactaaatg gtttaatctt gatatggctt gttttaaaaa tacatcaaga
     6901 cttgcacttt cactttcttt taacattcta taattgtatt tcttcttatc ttctcctgaa
     6961 attacaaaga tgatattgtc taaacttttt tcaacttcat ttaaataact gcttatatta
     7021 tatgataagt cagacaatag atttttaatc tgcttaaaca cttgtacatc aagtttagta
     7081 ttatactttt caaacaaatc tatttcttga ttttctaata ttcttggcat atatgctgac
     7141 aataaatcta aattgtttct ttgttcttgt atttgcttat aaagtgaatt tataacttct
     7201 attcttattt catcatttaa ctctttttct aaaatcttat caaattcaga tttcatatag
     7261 taaatatttc caaaaaaatt aaatattatc atatcattca tagctttaga ccaattctta
     7321 tatatcactt caatagaatt ttctttaagt agaggttgct ttgcatatat aaatacatct
     7381 tgcataagtt tttgaaccat atttttactt aagtttaaaa gtactttttg attattaaat
     7441 tttatttccg tttctacaaa attagaattt tcgttttcaa gttctttatt aattacaatg
     7501 tcttttatat cactattttt tatagatatg atttcttttt gaaaagactt tagaatagtc
     7561 aagctatcga aatcttcttg aataaaaaat ggagcatgtt ttaatgtata tcccactatt
     7621 tcattttcta tatcttttaa gtcatctatt tgcataattt ctctattttt atcatttata
     7681 tgtaggttaa atacattttt attatcatat aatattatat cttctcctaa atttaactta
     7741 gaacaattat ctttcgctga ctttacaata gtcttcttag atttcttatt tataaaaatt
     7801 aattggtctt gatattttac taaaagcatg tttgtgtttt tataatcaaa tccatctata
     7861 tttcctgata tcttgcagaa tactatatca caatctttag gaagatttcc tattttttgg
     7921 ttatctttaa ctctctcatt taaatcagat ggaactacac tatctatatt gttattaaat
     7981 atagatacag accttactat aatcccctct ctttccatat gaaagtatcc cttcaatgac
     8041 actcttttat cttctaaaat gtatctttct atatcatcaa aatttatttc aaatctattt
     8101 ttcccactga taaatataaa cttctcatta tctttattaa ttccacccat ataaaactta
     8161 ttgtcatcta aacttaatat agtcatatta tcctttagat agaatataaa ttctctttga
     8221 cttttgcaga ttaaatcata ttcatccaaa gttattttca tagtcaaatt tgctgtaaca
     8281 tttttacttg aattttcact ttttatgtaa aaaggcaagt tatttccata gacaattatt
     8341 tgagaatctt ctaaaactgc ctctaatttt tctttattgt ctgaaaaact atatatatat
     8401 tttacacctg tatcctcatt aacttcatct gatttgctga ttattatatt ccctagattt
     8461 gttatatgaa gctttttata ttcttttgag tatctactta tgccactaag ctcaaaccca
     8521 tcagaaagct catatatgat tttttctcct aatttcaaca taccttcacc acctaaacag
     8581 aattataagt attttataaa ggtgaataaa atgtctcaaa acaaataata tttttttaat
     8641 actttttatt ctgagacata gtttctcctt ttttataatg ctctaagcac cttcaactat
     8701 ttcttcataa agtttatcta aatgtttaac atattcacga tatccagatt tgttctttaa
     8761 ctcacttctt aaatcttcat atttttgttt aagttcttca gcactcaaag aaggagctga
     8821 ttcttttaat tcttctaact tataatcata gtacttatca tacatagcta tatatgtatc
     8881 aatagatttc ttagctagtt cattacattt ttcttgtgct tctccatatt gagaataatc
     8941 acctattttt attttatagt atccattttc ttgaattatt gttgaagaaa tacctttagc
     9001 ctcatattct tttgctgttt cttttgctgt tttttcaact ttaagtgaac ctgcataaac
     9061 agtgtaatat acacctttac catctttata tgcttccatt tcttttgctt tcttttcagc
     9121 ttgtgctttt tcttgagcct ctttttcagc ttttgccttt tcttctgctt cttttttctc
     9181 tttttcagct tgtgcttttt tctcttcctc agttaattta ttgttagctt gttgttgagt
     9241 tgatgacttt ggaatattct ctttatcatt tttcttattg ttgcctactg ttataaaata
     9301 tcctacacca acaataacca ctataactac tgctataata gtttctttta atctactctt
     9361 cttagaaaca acctcttcat catctaaaga atcttcttta tctgatacat aatttttcat
     9421 ttctttcttg ttttctctat atttgtcctt agaagaattt ttagattttt tattactatt
     9481 ttctttcttt ttaataggta ttactactga tttttcattt tctaattttt cttcttttat
     9541 ttcttttgct tttttatctt ctaagttttt actttctttt tcaagcttcg attcattatt
     9601 tgaagatact ttttcatcta agttattatt tattggtttt tctattttta cactacgttc
     9661 ttcttctata tgttgacttg gttcttcttg ttttatttct tttaattcat tttgctctat
     9721 ttcttctgat tttgcttgat gattttcaac tggtttatct aaaattatat caggagaaat
     9781 atcatttaaa ttctcttctt cttgttcaac ttcttcattg tcttttttaa taaatccaaa
     9841 taatttagcc ttctttttac tctttttatt ttctggttca attatatctt ctttattgct
     9901 gtcgtactct ttatctgtag tatctttatc ttctacaacg tcacctatat cttgattatt
     9961 actatctact tcttctttag ccccttcttc agcatcatct tctattgaaa tactacttct
    10021 aaagcaaata tctttatctg cattttttat catgtaaatg tattcttcaa tatcgatatc
    10081 tttatttctt ctagttcttt ttgatgcaat ttcatgattt tctaattcac tatagttatc
    10141 ttttattaga ttctgtgctt tattccatgt atcttcacta atttctgttt tatttttaat
    10201 tacatagtcc acaaattcct taaaattttc aggaccaaat tctccaatta tagtcagtct
    10261 cattgcacct tctataaggt ctctatttct tctctttaaa aattctaata caaccgttgc
    10321 acaagcaaaa tctttaataa tttctttgcc aaaaccatat ccttcaacag aatgaagttc
    10381 ttctaatcca cataacttct tatattcatt gtgtattgtt tgagatatac tttcattatt
    10441 taagctattt aacttatcta taaacttcga ttctatatca tttgattttg attcatgttt
    10501 tacttttgaa tttttaattt catttagtag catgtcattt tctgaattat ctttacctaa
    10561 ttgcaaatcc tcaatttcag ttgcttttat ttctactttg tcttgttcgt tattctcttc
    10621 cacattatta ggtaccacat ctttattgct agctaatatt ttgtttacac agaatataat
    10681 ttctcttttt aattctcttt tcgcatctgc ttgattttta agaaataaat ctccacattc
    10741 tttcttttct aaacttattt ttctaagaga tatattaagg aggtctttat ataggtttaa
    10801 aaccttataa tccttttcta aacgtcttct tctctctgat agttccattt tatcataagc
    10861 taaaaaacta tttaaatcat tggcttttag agagtctaat ccctctttca tagccttatt
    10921 tacatacttt ctacattttg ctgtagttaa ttctaaatct ttttctaatt catctatttc
    10981 aggtatgtac ttatctacta tccatttgcc aataagtggt tctgtatttc ttgctaatgt
    11041 cttataaaac aatatattaa attttaaaag ctctgcatat tttgcttcat taaattctac
    11101 tttttcaaat ttcttttcca aaacaccatc tagcttttca tttattttag actcttgttt
    11161 taaaaaactt gctgtgacat ttaaaacagt gtttcttatt atattatttt cactcaaaac
    11221 cttcactcct taaatcaatt attttattgt aatatttaca ttgtgcaaag ttgctaattt
    11281 gattatgata tctaaataat tatttactaa atttgctaca tcttatatca tatcatatta
    11341 tttacatcat tttacttttt ctttttatat ctatcttata ttataccatt tttaatatct
    11401 gtactaactt atattacaat attcctcaaa attttaaaag tagattagac aaatttacaa
    11461 ataaaaaaac atcagtttaa actgatgttt ttgactatcg aaataattaa gcaagtctat
    11521 aagcggagtt ctgtattaga taatcatctt tctaggccta ttgtcaccaa taggctcaag
    11581 cgacctacct accggagagt acgagcagca ctctttattc ctatcttggt cttgctccag
    11641 gtggggttta catagccaac tagtcgccta gctgctggtg agctcttacc tcgcctttcc
    11701 atccttacca caatagtggc ggtttatttc tgttgcacta tccttaggat cgctcccact
    11761 aggcgttacc tagcaccctg ctctatggag ctccgacttt cctcatgtaa gattacatgc
    11821 gattatctgg cttacttaat atttatttta cttagttatg ttaacacaaa ttcttttaca
    11881 tgtcaaagta atattatact aaaaatttaa atttattcaa aaatagtgta atatagatat
    11941 ataaactcca tttaactcga ggtgatatat tgaaaaaatt atatagaata gtaattaaca
    12001 ttatactagt attagtaata ctttacagtg gatttaatat atactcaaaa cttaccaaat
    12061 acaatcatga tactaaaaca tcctcagaac ttcaaaaaaa agaatataaa aaagaggatt
    12121 tatcaaagat taacagtgat tttaaatttt ggctttctgt tgagaataca aatataaatt
    12181 atccagttgt acaatctaaa gataattctt actatcttga caaagatttt tataaaaaag
    12241 attctatttc tggtacactg tttatggatt atagaaataa atctattgat gacaaaaata
    12301 tcataatata tggacataat atgaaaaata aaactatgtt caacaaccta aataagttta
    12361 aagatgctga cttctttaaa aacaataata aaataaaaat aaccttgaat ggaaaagaat
    12421 ttttatatga tgttttttct gcttatattg tagaatctga ttatgattat ctaaaaacaa
    12481 attttaacaa tgaatctgat tatcaaaatt acataaatga tattacttct aaatcattgt
    12541 ataaatctcc tattaaggta aatagcaatg ataaaatagt tacactttct acatgtacct
    12601 atgaatttga tgatgccaga atggtgattc atggtagatt gatttaaaac tcacagacac
    12661 agtttaaaac aataatttta aactgtgtca tcttttatac ctataattta taaattaggc
    12721 cactaaaagt atgtaataca aaataacaaa tacatcaaac aataaaaaac aataaaaaaa
    12781 gactatgcta aaaacatagt ctttactgga gctggtgata ggaatcgaac ctacaacctg
    12841 ctgattacaa gtcagctgct ctaccgttga gccacaccag cacacctatt ctattgtaaa
    12901 ttagtaaaac taatttttaa aagttaacac tctaaaaact acacatatat cttaatgaat
    12961 gaacatcaaa t
//
LOCUS       DAEPOZ010000112        12925 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.112, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000112 DAEPOZ010000000
VERSION     DAEPOZ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12925
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.112"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(67..2037)
                     /gene="ftsH"
                     /locus_tag="KR440_002249"
     CDS             complement(67..2037)
                     /gene="ftsH"
                     /locus_tag="KR440_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBH0699293.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(2071..3459)
                     /gene="tilS"
                     /locus_tag="KR440_002250"
     CDS             complement(2071..3459)
                     /gene="tilS"
                     /locus_tag="KR440_002250"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBH0699294.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDEN
                     TKEVLKINFNKL"
     gene            complement(3642..4070)
                     /locus_tag="KR440_002251"
     CDS             complement(3642..4070)
                     /locus_tag="KR440_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBH0699295.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEENIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(4087..4893)
                     /locus_tag="KR440_002252"
     CDS             complement(4087..4893)
                     /locus_tag="KR440_002252"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBH0699296.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(5095..5580)
                     /locus_tag="KR440_002253"
     CDS             complement(5095..5580)
                     /locus_tag="KR440_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HBH0699297.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(5631..6290)
                     /gene="spoIIR"
                     /locus_tag="KR440_002254"
     CDS             complement(5631..6290)
                     /gene="spoIIR"
                     /locus_tag="KR440_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HBH0699298.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(6435..7115)
                     /locus_tag="KR440_002255"
     CDS             complement(6435..7115)
                     /locus_tag="KR440_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0699299.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(7099..7989)
                     /locus_tag="KR440_002256"
     CDS             complement(7099..7989)
                     /locus_tag="KR440_002256"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBH0699300.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(8107..9657)
                     /locus_tag="KR440_002257"
     CDS             complement(8107..9657)
                     /locus_tag="KR440_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HBH0699301.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVVQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(9751..10017)
                     /locus_tag="KR440_002258"
     CDS             complement(9751..10017)
                     /locus_tag="KR440_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBH0699302.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(10165..11025)
                     /gene="yabG"
                     /locus_tag="KR440_002259"
     CDS             complement(10165..11025)
                     /gene="yabG"
                     /locus_tag="KR440_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HBH0699303.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(11109..12047)
                     /locus_tag="KR440_002260"
     CDS             complement(11109..12047)
                     /locus_tag="KR440_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HBH0699304.1"
                     /translation="MKFIDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(12157..12318)
                     /locus_tag="KR440_002261"
     CDS             complement(12157..12318)
                     /locus_tag="KR440_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699305.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(12328..12825)
                     /locus_tag="KR440_002262"
     CDS             complement(12328..12825)
                     /locus_tag="KR440_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBH0699306.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
BASE COUNT         3828 a         2461 c         1252 g         5384 t
ORIGIN      
        1 tatactttca gttttagtat ttaatccaca tcagataata atcttaaaat gaccaaggtc
       61 ttaaaactat ttatctttat cagatttatc ttctaaattt ttatttatat caataacatt
      121 gtttctttct tcttcatctt tttctaattt tatagttaat tgttcttcaa cttctttaga
      181 agaattttcc tctgtaactg cattttcaat ttcattcaga ggtaactctt tattaaatgc
      241 ttttacaaat tgttctgcat ctaaagtttc atatataagt aaagcttgtg ctacatactc
      301 taatctatcc atattttctt gtaatatact tcttgtctta tcatatgcac catcaactat
      361 acgctttgtt tcttggtcta tctcaaaagc aacttcttca gagtaatttc tcttacttga
      421 aaaactatta cctaggaata cttcatcatc actgtcaaat gacattggac caagtttaga
      481 actcattcca tactttgtta ccatacttct tgcagtagct gtaactcttt ctaagtcatt
      541 tgaagcacct gtagatacat ccttaagtac tagctcttct gctactctac caccaagtaa
      601 aactacaata ttttctttca tttcattttt tgtagcatag aacttatctt caacaggaag
      661 ttgcattgta aaccctccag ctcttcctct tggaactatt gtaacttgat gaacaggact
      721 tacttcttca agtacatggg cacaaactgc atgccctcct tcatggtatg ctgtaagtct
      781 tctttctttc tcacttatta ctttcgattt tttagccaca cctgctataa cctttgttat
      841 agcttcttct atagtttcca tcttaatttt cttttctctt tttctagctg ttaatattgc
      901 agcctcattc attaagttct ctatatcagc aggagtaaat cctggtgttc ttctagccaa
      961 gacatccatt tttacatcat cacttaatgg tttatttctt gagtgaactt taaatatagc
     1021 ctctctacct tttacatctg gtgtaccaac aacaacttgt ctgtcaaatc ttcctggtct
     1081 aagtaaagct gggtcaagta tatctggtct atttgtagct gccatgatta ttataccttg
     1141 atttacccca aatccatcca tttcaactag aagttgatta agagtttgct ctctttcatc
     1201 atgacctcca ccaagacctg cacctctttt tctaccaact gcatcaattt catctatgaa
     1261 tatgatagct ggagcacttt tcttagcctg ttcaaataaa tctcttactc ttgaagcacc
     1321 aaccccaaca aacatctcaa cgaaatcaga accacttata ctgaaaaagg gaactcctgc
     1381 ttctcctgca actgctcttg atagatatgt tttaccagtt ccaggaggtc ctaccataag
     1441 cattcctttt ggtattcttg cacccaattc aatatatttc ttaggattct ttaagaaatc
     1501 tactacctcc tgcaaatctt ctttttcctc atctagtcca gctacatctt taaatgtaac
     1561 tctagttttt tcatcatctt tatgaacctt agcttttgat ttgccaaaac tcataacctt
     1621 tccgcctcca ccttgagact gattcataaa cacgaaccaa ataatcacca tgaagaatat
     1681 taacagtaat gtaggcaaca tttcgacaaa ccaaggtgta gatggcttag cttcaccacc
     1741 aaaagttagt ttcccagcct tagcttggtc taacacctca tctgcaagtt tatttcccat
     1801 cacttctgtt ggaacatatg acttaaactt tgtatttgta tcttttatag taccttctac
     1861 agaagtttga tttacaaagt ataatcttga tatattctca tctgttaatt ctctatatac
     1921 ttcagagaat tttaaatctt ttattttttc tgtaggctta cctgagaatt gaactatacc
     1981 tactataatt ataaaaacaa gtagataaaa acctgcccct tttagcaact tattcaatat
     2041 agaagccctc ctttcataca ttgctttgcc ctataatttg ttaaaattaa tttttaatac
     2101 ttcttttgtg ttttcatcaa ttttataatt ttcactgatt ttataatctc caacacatat
     2161 tattccttca ctatctgtga ttataggaat tttacatctt tcttctcttg ggatttttaa
     2221 atctatgaat aaatctttca cttttttact tcccatagca agctttatct tatcaccttg
     2281 ccttctgctt cttattacta tacccccttt taccttatta aaatcaaacc ctttagatga
     2341 attgtctaat ttcatactct tatacctatc tatgctcatt acttgggtct caataatttg
     2401 ttttaattct tttattttaa taaacccatt actaggtaca ttgtaataaa attcaatttc
     2461 ctcattaaca atttcctcat tcgttaatat tatactgtct ttttttctat aaacaaatat
     2521 atttcttgga agagttaaca ttttatttac ttttgaatta tcttctaaag acattatatc
     2581 ttctatatgt ctttggtcaa caaaattagt atctcctagt atatgtttta tggaatttct
     2641 aagaactcta actttgatag catcatgtaa gcaaacaaaa tcgtctaaat ttatctctac
     2701 aaagcctttt tcttttatat ttgaaacttc tctaaattta gcttctgcct ctttctcaat
     2761 ataatcgtta tcacttttta aactattgct catcctcact atagattcta ttacatttga
     2821 attaaaatta tccttcatat atggtaaaag gtctaacctt atcttatttc tagtgtatat
     2881 attttctaaa tttgttttat ctatcctcgg atttaaatta taagcttcac aatactcctc
     2941 tatctcattt ctttccacgt ctaatatggg tctaattatg caattatctc taatataatc
     3001 aattcccttt aagcctttta agcctgttcc tctcatgata cgcatcataa cagtttcggc
     3061 ttggtcattg agattatgac ctattgctat tttattggct ttaatcttat ccttaatttc
     3121 ataaaacatt tcatatctta gcttccttgc tccttcttct aatgataatc cctcattttc
     3181 acaatatttt ggtacattaa tagactttac aaagaatata attcccatat cctcacaaag
     3241 tttagataca tataatgcat ctttttgagc ttctatccct cttatttgat ggtttaaatg
     3301 tgctgcatat atttctatat taaaatcttt ttttaatcta tttagtacat gcaataaaca
     3361 aactgaatca ggtcctccag aaagacctaa aactatttta tcgccttttt gtattaaatt
     3421 atgtttattt atagtactta gtaccttatc aaatatcata tttatccctc ttacacttag
     3481 ttttattcac ttttaagtat actttctata aaaaaacgct aaacccttgt atcaatttta
     3541 agaaattaaa gtttaatata aatataataa taccaaataa attaaataaa aggtagtttt
     3601 ttcactacct ttataaaatt tctggaaaat agccatagtt attatttagt acctatgtat
     3661 atatctattt tattcctacc taaaaataaa tcttgtacct ttatattata gtctattttc
     3721 aattttatgt gttccccttc ttgtatattt atatctaatt ctcttgtatc aatatcaatt
     3781 aataaagtac cttgtggggt cacatacttt gtagtactac tttctccaca tttaaacagc
     3841 attgttgagt taatagcacc aaactttttg atactaactt gatttttaag tatctttata
     3901 gtattagtta tttctatatt ttcttctttt tccttgtaaa taacatatat tccatcattt
     3961 ttataaaaaa ttttaccaaa ggcagtcatc tctatggtgt ccatatttcc tttttcatca
     4021 tattgtctag tatttatact tatttttaca ctcaacgatt tttcctccat agattcttca
     4081 tataaactag tatttttgta tttctacttt ttgaatctta tcaatttttt tctttaagaa
     4141 ctctcttgct atatctgaaa atttttcagg tatatctgat acataatatt gataagtccc
     4201 atgaatttct gtattgttta taatattttg cacctctaaa atctttttta aatcctttgc
     4261 agtctcttta gctggattaa caagttttat atgctctcca acttcttcac ctattgttct
     4321 ctttaaaata ggataatgag tacatcctaa cactaaagaa tctatatttt tttctttcaa
     4381 ttcttgtaaa tatatctttg ctgtcaattt agccacttct gtatttgccc atccttcttc
     4441 aacaataggt acaaataaag gacatgcttt attaacaatt tctatactct catctatttt
     4501 agatatttcc aagttgtatg cttttgaact tatagttcct tcagttccta ttattcctac
     4561 aattttgttt tttgtagaac ttacagctgt tctagctcct gcttctataa ctccaattat
     4621 cggtatatca tatttttctt gagcttcttt taagcttctt gcagtagcag tgttacatgc
     4681 tataactata gcttttacac cttttgtttt taaaaagttt atagcttgaa aagtatattt
     4741 aattatagtt tctttggatc ttgaaccata tggtattctg gcagtatctc caaagtatat
     4801 tatatcctca tttggtaata tcttcattat ctcttttaga actgttaatc cacctaatcc
     4861 cgaatcaaaa actcctattg gtttattgtt catctttata caacacctac tttacatttt
     4921 tgtgtttaac aacaacttat ttatcgctaa aatttatatt tatattcata attaatatat
     4981 tattacctaa atgattatta tagtttattt tacacaaaaa ataaaggagc tatcttaaat
     5041 tagaaaataa tttctacaaa aagacaactc ctttcactac ttaaaaattt aaatttattt
     5101 tgcaaacaca tgatttccta ttctcatata aggattcaat ctatttatcc atttgcaagt
     5161 ggctatttct ggattccaaa aatatactgc tttattagtt gggtcagttc cataaagagc
     5221 ttcctgagct gctttacgtg ctgagtctgt tggtgccatc tttattgaac catcaagcac
     5281 aactgagaaa gcattttttt gatatatgac ttcttctatt gtgtctggaa atctagggtc
     5341 caatactcta tttattacta cagctgctac agcaacctgt ccctcatagc tttctcctct
     5401 agcttctcct gcaaccaact tagataataa catcaagtca ttatcagata aatttattac
     5461 ttctttcttt gctatatttt tttcttgttt gtttactttc ttaatatttt ctgttggttc
     5521 atatgcaaat acctctttaa taggcataaa tataaaagtc aaagctaata gtgtagccat
     5581 cagtttactc cacctgtcca tgattaaaac cactccttac attcttattt ttaaaatttt
     5641 ttctctaaat ccttgactac ttcaagtact ttaaacttaa tttttactct acttgttttt
     5701 tttggtgtat cttgttttat taacttatat tctttatcag ttaaaacttc cttcaactta
     5761 tcatctgtag atttatctat cactccatta gattcatcaa caaaacatag tggagggaac
     5821 ataacacacc accagttttt tccttcacct ttcccaatga taatctttaa tgctttatat
     5881 tcaccagctg gaagaactat attagaatat tgtttagtag ggaaattgct atattgtata
     5941 cctaccttta cactgtaatt atatccattt ttaagaataa tatttttact tatttcttcc
     6001 agactactat attccttttt tataatagct tcactttctt ttatactttt tgaattttga
     6061 agctttggct gtaaatattt tattacttca tctctaactt ttagtttaag ttcttgatct
     6121 tcatctgtat tgctgtttgc tataacatga aatcttatta atttatcttt ataatctttt
     6181 gatatattgt ctactttttt tacctcacca tttattacta ttgtcattat tgatatgact
     6241 gatataagac ttaatattaa tagtcccaat ctaatcttaa tttttctcat tgaaaattcc
     6301 ccctcaagat aaatttatat catcaattat gtccaattaa atttaattta ttcataatat
     6361 tttttaatac taataaaaaa agctatctta aaatgattac aatcatttta aaatagcttt
     6421 ttaaacctta tattttataa gtctttacta atcatagcct tatctatcat aaactgctct
     6481 gctttttcta tatgctctgt agcatacttc ttggatagct cactatcacc tttaattata
     6541 gcatcaagta agtatttatg ctcttcaaca atattatgtg atgtatcata agcagacata
     6601 taggtaactc taaatctata tacttgttcc cataatgagt ttattagttg gattaatttt
     6661 ttattattag ttgctttaaa tatacattca tgaaattcta catcaagttt taataacgca
     6721 tctacatcgg attccaaagt gcttttttta aattcttctg atatatcttt aagcttttta
     6781 atatcttcat cactaattct ttcagcggca agatatgctg ctaagccttc taggcttgct
     6841 cttacttcta atacatccat aatatccttt aatgacatat ttgccacata cgcacctttt
     6901 ctaggcaaca taacgactaa accttctaat tcaagctttc ttattgcctc tctcactgga
     6961 gtcctactta ctcctaattg ttctgctagt tgcacttcca ttagcctttg accaggcttt
     7021 aattttcctt ctaagattgc ttctcttaaa ttctcaaaga ctacatctct taatggcttg
     7081 taattatcca agttcagttt agttaaattc tccacaaatt tcaacccctt tttgactgct
     7141 attaacaaca tacacctgtt tatatttttt taataattct ttttttccaa ttagagcatc
     7201 ttctttattt ttaaatagtc caaaaactgt aggaccactt ccactcatca tagaacctaa
     7261 agcattattt ttcatcatta cttgttttat atcacttatt tctttgtgtt tacctatagt
     7321 aacattttca agaatattta ccatgctttc tgatacagct tttatatctt cagattttaa
     7381 acactctatc aaatatttat tatttggtct ttttttaata ttattcaaat ctaatccttg
     7441 ataaacttct ttagtagaaa cgaataagtc tggtttgcat atcaatatat ttgtatcaca
     7501 tggtaaacct ttgatgtttg ttagcttttc tccaatacct tgagctaagg caggtcttcc
     7561 tgatatacaa aaaggaacat ctgcgcccaa attaagtcct atttctttta attcatcttc
     7621 agataatcta agttcccata ggtgatttaa acctactaat actgcggctg cattagaact
     7681 tcctccagcc atccctgctg ctacaggaat atttttttct atgaatattt ctacaccttt
     7741 ttttatataa aatttatttt taagtatttt ggcagcttta taaacaatat tatcttcatc
     7801 taaaggaata tctaaactag tgctttttac ctttatttca tcttcgtcta attcttttat
     7861 ctttactata tcgtataaat ctatcgtttg cattatcatt tcaacaaaat gataaccgtc
     7921 ttgccttttt cccaagacat caatggataa atttattttt gctctactct ttagtcttat
     7981 aaaatccatt ctatcctcct tgtttaaaga ataatgcatc actaacaata atattatact
     8041 acaaaaaata aaataatagg gtattataat accctattat acttaattac tttaaatttt
     8101 ttaatattaa tctactaata cgactttttt ttctaaaata agacacttac caggcttaat
     8161 tggttcatca agttttatat cattaagttc tgcaatttca ttttctgttg tattgtattt
     8221 ttttgctata ttccagaaag tatctccttc tttgcatata tatacgatta tgctaggagc
     8281 tttagataaa tcatatacac cttggtcttc tcctttgata ataaaatttt ctgctttctt
     8341 atctaatgct tcagtaaatc tcttaatttt gataatcaag tctatctggt ccctattcaa
     8401 atcaacttcg actttatcaa tacatgctgt attaaacaca gatgctgtat cagttagatt
     8461 atccatttct atatcatgtt caaatggaat ttcctcacta attttataaa ccaattttaa
     8521 accttcaact gggacaaata aaatttcaac ttttatgatt ccttgtataa tgcttttatt
     8581 tccttcaata taactatttt ctatagacat ggttggacaa acgcttacaa tatcttttat
     8641 ttgtatatca tcattatcat ttcttatgcc ttctctaacc ataaatgttt cttcactacg
     8701 tcttaaagtc ttatttaatt gaataggttt gtggtcaaat tttattatct tttgaggcga
     8761 atatgcatct tgtaatactt ctcttgtaac ttcatctgta acttttacct tacaacaagc
     8821 catacaatct atttctaaaa gacctgtgtt gctttcacta ttttgcttga aaatatgatt
     8881 aaaatctgac atagacaaca atacttcttc agtcatacca tcactaactc cagggacttc
     8941 tacaaattga gtaaactcaa tacctactct atccaactct acaagttcac cctcatatgt
     9001 acaagctaac ggatttattt ctaatactcc accaataata actttattgt cagtcactct
     9061 actttctttt actttaacac atggatttaa acttattata gattgaattt cctctgtgtt
     9121 tattgttata gtatctctta tagaactttc tgctttttct attccaacta tatcttgaaa
     9181 acaaatttcc tttctgtgct tttgtatgcc ttccacttgc gccacatctt ttacaatgtc
     9241 taacctttgc ttttcaaaca aacttcccct tatattcata agagctccta ctttaatctt
     9301 tctctcattc attatagtac aatccatatg ctctacttct gaaaataaca tgtattccat
     9361 atcttgaaca acattgtctt tttctattac ttcgtttata tctacttttc cgtctacatt
     9421 agaaatagta tttttatcat ctgcaatata gattacatta taattaaaac tacctctgca
     9481 aagtatcttc ccatctgcca tttcaatttt tttaagtgat atgtatcctt ctgtttttac
     9541 aatttcatat acatctaatt ttttgtctgg tacaactgcc tccgcttcaa tgaatgtttg
     9601 aaattttcca aagtctattc tattgtcaac tttaattaca tctttaatta attccatagt
     9661 ctacctcctt aagtattata aaaatatcac actatattta tatttaagga gcaaaaaaaa
     9721 aattacacct atttttaaat aggtataatt ttaacttatt tgtaatttat cttgatctct
     9781 aaaaacttgc aactttacat tagatgttaa taaatctgag taactataag atactctagg
     9841 atatccatta ctttcatcat ctaactttat aacaaacacg cttggataaa ctttttctag
     9901 tataccctct tttgtaataa tctgctttct tcctttatta gctttcaaca atattttctt
     9961 tcctatatgt ctctctaagc ttactcttat cttatctaga gtttgaacag tagccacaat
    10021 atcaccttct ttaacgttta tatgtttaca taatatcaca tataaacatt tttgtcaaat
    10081 gtttaaacta attattttat atattttttg caaaaatgtc aatactattt ttgaagatat
    10141 acagtataat tatgttaatt tttgctaatg taatattgtt tttggcattg cccatctaaa
    10201 ttttcctctt gtttcagaac caccaattcc cttaacacct gtaatagtct gctctataac
    10261 ctcatcaata ggaactacta catcaattct tgaacaagca attttctcca ctataaaaac
    10321 agggtctaat gcatgtatca ttattcttcc aggcgaactt gcataatttg ctcctgcact
    10381 tattattttt tcataatttg attggcatgc tcctgcgaat atgacaagat tatccaaatt
    10441 tggttgccat ttacgtgctt cttttactgt tttaacaaaa tttgaagagt ttctatagtt
    10501 actcatatct tctatatctc ctctttttat tgtcattgca tcatgacctg ttattactag
    10561 tatatccgga ttatattttt ctagtagtgc tcttacttct ttatactgat tttgctctga
    10621 aacagctaca cctactgcag gtattccaag ttgagcatat acatccaagc aaatttttaa
    10681 atattcttta tctccatcta tctgtagtac ttttccaggc attccatatg tgttaggatt
    10741 taattgaagt tttggaactg ctctagtcat tcttttttgt ctttctttag cttttcttac
    10801 tgacttatat aataaatttt ctacattttt atctattaat atatctttta tatcaggtgc
    10861 ttttactaat tctaggtcat caatataagc atctgctatt actctaaatg caattccctt
    10921 tagtatagct attttctcat tattttcatc tacaccaaaa gagactattt taaatactat
    10981 atctttgtta tgtgactttc tagctacaat gtctcctacc ttcatagtta aacctccaat
    11041 aaatttttat cttagtttat actatgttcg atataaaact ttgtttccta atgtcaaaaa
    11101 aacatttctt ataatacttc ttttatcact cttaaagcat ttttatagta tatcttttct
    11161 atatcatctt cactaaaccc ttcttttttt agaggttcat acagtttacc catctgagat
    11221 atatcttcaa tctcaacttt tgaatctatt ccatcaaaat ctgaccctag ggatacaaca
    11281 tctattcctc caacatttac aatatgtttt atgtgtctaa ccatatcttc aagtttactt
    11341 tcacttttat cacttaaaaa taagtggaag aagtttatac ctgtaactcc acctttattt
    11401 gctaatactt taatcatatc atcagttaaa tttctagaat gattcatcat tgctcttgaa
    11461 ttagaatgtg tagcaattat tggtttagaa gaaatctttg caatttcata aaatccacca
    11521 tctgaaatat gagaaacatc aaccaacatt cccaattcat tcatcttatg taccacttct
    11581 cttccaaaat cagtaagccc tttttctcta tattctactt tattatgagg aaagctcaat
    11641 tcattcacat gattccaact tattgtcatc attctaaccc ctaagtcgta aaatttcttt
    11701 aaattttcta atttaccttc aagtactgca ccttcttcaa tagataaaag agctgtaagt
    11761 ttaccttctg attggttttt cataatttct tcatagttag tagctaaagc tatttcatca
    11821 ctattttttt tcatttcctc atgaaattta tttgccatac tcatgcaata atcaaaagga
    11881 tgttttactt cttcagtatc aacaaaaaga gcaaatgttt gagctaatga atcccccttt
    11941 ttcaatctgt ctatatcaac agaatattta ttagacttaa gttcacttgt ttctacattt
    12001 tccattaatt ttgctatagt atcacaatgt aaatcaataa atttcatatt tataccccct
    12061 gtatatttta tatttatatc taaaaataga aatagttgtc cttatagaca actattgaca
    12121 actatttttt aatattcttt ttaatattac ttaatcttat tcttctttta agttaggtat
    12181 tactttttta acttcttcaa tttgatgcca tgttttatct atattatttt cactaatata
    12241 tttttttaaa tcagttaaag atgtaaaatc tttaactgat aaattgtcat catcatctgc
    12301 aaatcttaaa ataaacatga gactccctta ctttccacaa catttcttat atttttttcc
    12361 gcttccacat gggcatgaat catttcttcc tatttttgct tctttaacta ttgttttaga
    12421 cttcttataa gcctttatta tttcttttct ttggtctgct ggtagtatat cttcccaacc
    12481 ttgtaaatta tatagccaat gagcctcaac agttaccata ttgttatata atttttccca
    12541 atctattttt atagaaactt ctttatcagc ttctacttct tctaaaacta tatcttcttt
    12601 taagctttca cttattccat ctatgaatcc catgaaaaat tcattagtag ttccaaattt
    12661 ttcagccaat tcacttactt taccttcaac aacatccatt ttattattta atatttcatt
    12721 gtatattcca gcttcaactt tcaaatattc ttcccagaat tttacttctg cttcttgact
    12781 ttcatgatta tcacttaaat ttctccattc agtatataaa ctcatacttt ttcctcctaa
    12841 gtatacctat atttttatag tttttatagt ataaaatctt ttagttagat tggaagtttc
    12901 ttctttttga ttataccata ttcta
//
LOCUS       DAEPOZ010000113        12700 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.113, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000113 DAEPOZ010000000
VERSION     DAEPOZ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12700
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.113"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..148
                     /locus_tag="KR440_002263"
     CDS             <1..148
                     /locus_tag="KR440_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0699307.1"
                     /translation="DLDIINDIIISFYQNLLFLKKNTGKNSGDDELTMKMISSIEYII
                     KHAK"
     gene            complement(305..1324)
                     /locus_tag="KR440_002264"
     CDS             complement(305..1324)
                     /locus_tag="KR440_002264"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBH0699308.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVINKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(1355..1969)
                     /gene="plsY"
                     /locus_tag="KR440_002265"
     CDS             complement(1355..1969)
                     /gene="plsY"
                     /locus_tag="KR440_002265"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBH0699309.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(2004..3323)
                     /gene="der"
                     /locus_tag="KR440_002266"
     CDS             complement(2004..3323)
                     /gene="der"
                     /locus_tag="KR440_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBH0699310.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(3357..4685)
                     /locus_tag="KR440_002267"
     CDS             complement(3357..4685)
                     /locus_tag="KR440_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HBH0699311.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEIGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPENILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(4923..5123)
                     /locus_tag="KR440_002268"
     CDS             complement(4923..5123)
                     /locus_tag="KR440_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699312.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(5125..6057)
                     /locus_tag="KR440_002269"
     CDS             complement(5125..6057)
                     /locus_tag="KR440_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBH0699313.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNM
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(6102..7034)
                     /locus_tag="KR440_002270"
     CDS             complement(6102..7034)
                     /locus_tag="KR440_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBH0699314.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYDLSFFHCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNDVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(7089..8378)
                     /locus_tag="KR440_002271"
     CDS             complement(7089..8378)
                     /locus_tag="KR440_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0699315.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(8407..9099)
                     /locus_tag="KR440_002272"
     CDS             complement(8407..9099)
                     /locus_tag="KR440_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0699316.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(9114..9875)
                     /gene="pgeF"
                     /locus_tag="KR440_002273"
     CDS             complement(9114..9875)
                     /gene="pgeF"
                     /locus_tag="KR440_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HBH0699317.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENITSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(9872..10333)
                     /gene="nrdR"
                     /locus_tag="KR440_002274"
     CDS             complement(9872..10333)
                     /gene="nrdR"
                     /locus_tag="KR440_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBH0699318.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIEEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(10355..10618)
                     /locus_tag="KR440_002275"
     CDS             complement(10355..10618)
                     /locus_tag="KR440_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBH0699319.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(10722..11495)
                     /gene="sigG"
                     /locus_tag="KR440_002276"
     CDS             complement(10722..11495)
                     /gene="sigG"
                     /locus_tag="KR440_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HBH0699320.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(11587..12309)
                     /gene="sigE"
                     /locus_tag="KR440_002277"
     CDS             complement(11587..12309)
                     /gene="sigE"
                     /locus_tag="KR440_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HBH0699321.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(12334..>12700)
                     /locus_tag="KR440_002278"
     CDS             complement(12334..>12700)
                     /locus_tag="KR440_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HBH0699322.1"
                     /translation="KALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDID
                     MKKAQEIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGIS
                     NFNDREYGAIMNPNILPGF"
BASE COUNT         3869 a         2406 c         1247 g         5178 t
ORIGIN      
        1 agatttagac ataatcaatg atataattat ttctttttat caaaatctat tattcttaaa
       61 aaagaacaca ggaaaaaatt ctggtgatga tgaattaact atgaaaatga tttcttctat
      121 tgaatacata ataaaacatg ctaaataaaa ttaaaatatg gctgtctcaa aatagagata
      181 agcctttttt attgttgaaa taataaaaat atatttaatt tcatataaaa aaaagtgcct
      241 cgtaaactaa aaagttcatt ttgagacacc cctaaaacaa atttattatt tattatacaa
      301 tagtttatat atcatcacct aaaactactt cttccatttc atgcttttta cttctcatca
      361 ttagttctat tccaacttct ttaggatttg aacctttatt tattacagaa tatatagcat
      421 tagttatagg catatctata tctaattttt cagcaacatc atgtgctact tcagtagcag
      481 ttatgccttc tacaaccatc tttacttctt ttaaagtatc ttctaaactc atacctttac
      541 ctatgagtat accagctctt ctatttctac tatgcatact tgtacaagta actattaaat
      601 ctccaatacc tgaaagacct gcaaaggtag acatattagc tcccatagca attccaagtc
      661 tgctcatttc actaatacct cttgtcataa gggcagcttt tgcattatca ccatacccta
      721 atccgtcaca tatcccagct ccaaatgcta taatattttt taatgctcct ccaagttcta
      781 ctccaactat gtctggattc gtataaactc taagtttagg gctcataaat aaatcttgta
      841 tcatctgtgc tatttttaaa tctttagaag caactactac ggtagtagga atatcccttg
      901 ctacttcttc agcatgagat ggcccagata gtattacata tggattttgt ggtaattctt
      961 cttcacagac ttgagataat ctaagcccag tccctttttc aagaccttta gctacattaa
     1021 caataacttg tccttctttt ataaatggtt ttattttttg gcaagtactt cttacagcct
     1081 gtgatggaac tgctaaaact attaccttac aatcttttat agttttttct atatcagtac
     1141 ttatagttat attgtttgga aataatactc ctggtaaata gtctgtattt tctttagttc
     1201 tgtttatttt tttagcttgt tcctcttttc gagtccacat gctcacatca ttaccttttt
     1261 ttgcaagccc taaagctaat gcacttcccc agcttccagt acctaataca catactttct
     1321 ccatgtttaa caccacctga gtataatata tttactattc tttcttttga ccaatttttc
     1381 tctctgtccc atttaataat cgctttatat ttgctctgtg attatatatt actgatgcag
     1441 ttaagaacag agctactatt aaaccagctt tattttttac cataatcata aatattggtg
     1501 aacatcctat tcctacaaca gaacccaatg aaacatactt tgttatagct accaccaata
     1561 taaatactgc tagacacatt agtgttatta ctggatttac agctagcatt gcacctagtg
     1621 aagttgcaac acctttacct cctctaaatc ctaaaactgc tggccaatta tgacctgcta
     1681 caacacatat aactgccaga tatccagcta aaagagtatc tattccaacc aatcttgctg
     1741 caaattctga tattaaaaca gccattacac ctttaagaac atcacctaaa aatgtcattg
     1801 ctcctgctct ttttccaagt gtcctaagta catttgtcga acctgcatta ccagaacctt
     1861 gagtccttat atcaacccca gcaattcgct ttgcaactat gtatgaggta gatatatttc
     1921 ctaaaagata tgcaactaca gcaataatta tataactaaa tatctccact gaatttcccc
     1981 cctttagtta tattttaaga tatttatttt ttattttttt gtctatactc aaactttatg
     2041 gaagttcctt caaatccaaa attttctctt atcttattct caaggtatct ttgatatgag
     2101 aagtgagtta aatccttatc atttataaac aatgtaattt taggaggtct aatgtcagtt
     2161 tgagtcccat agtaaatttt tagtcttcta cctttgtcag atggtggttg atttaacatt
     2221 acagcttctc caattacttc attcagtgct gaagttgata ttctcttaga atgttcattt
     2281 gacacatatt caactgtatc tagtatcttg ttcattcttt gattagtttt tgcagaaaca
     2341 aatacgattg gagcatacat cataaatgga aatttttctt ttatatcttt tgtgtaatta
     2401 ctcatagtct tattatcttt ctctattaaa tcccatttat tgattacaaa tatgcatcct
     2461 tttccttcat catgtgctat acctgcaacc tttgtatctt gttctgttac accttctaag
     2521 gcatctatta ctatcaatac tacatctgct ctatctacag cactcatagc tcttattaca
     2581 ctgtattttt ctattgtctc atatatttta ctttttcttc taagccctgc tgtatctatt
     2641 agaagaaatt tctgtccatt tttttcaaaa taagtatcaa ttgcgtctct agtagttcct
     2701 gcaattggac ttacaattac tctttcttct ccaagtatct tattgagtat agaacttttt
     2761 cctgcatttg gtttacctgt tatagctact cttattatat cttcttcata ctctgtatct
     2821 aatcctgctg gaaaattttc aactatctca tctaataaat ctccaaatcc catactattg
     2881 gctcctgaaa ctgcaaaagg cgttccaaat cccaactcat aaaaatcata tatattatca
     2941 aattgacttt gactatcaat cttattaacc actaagatta ctggtttttt tgtctttcta
     3001 agcaattgtg caatttcttt atcagcagct gttattccag ctttaccatc aactataaaa
     3061 agtattacat gtgacatgtc cattgcaagc atggcttgat ttctcatttg agataatatt
     3121 atatcttcgc tatcaggttc aattcctcct gtatccacta atgtaaaata tttatctaac
     3181 cactctactt cagcaaatat tctatctctt gtaactccag gtgtattttc aactattgat
     3241 atcctttttc ctgcaaattt attaaatatt gtagacttac caacgtttgg tctcccaact
     3301 acagctacaa ctggcctact tatactcatt ttatttctcc ttccatcttt tgttttctat
     3361 tttaaaattt tatccacaaa gtcttttcct tcattcaaac aagggataat ttttattctc
     3421 ataagttctt ctaattgatt taattcaata ttatctaaga aaatttcttc atctgctttt
     3481 agcatacttc ttgttatata aagagcttct cctaattctt tatcttcaag ttgttctttt
     3541 aaatccttag ctgttaaaag acctgataca gttattgtct ctccaaaaaa cttgtttttg
     3601 atttcatata catttacttg aagattttta aacttgtcca tcatagcatc tgccatactc
     3661 tgtataaatt catatgcaga atgaccagta gctattgata ctttcttctc tctttttaga
     3721 atattctcag gcaatatatt tagatactct tcaatctctg tttttaactt acgtatcata
     3781 cctacaccat cttcaaactg taaaaaacct tcatattctt catatccagg caattcacta
     3841 ttagatagta tataaaattc atctgataaa aatgcaaatc gagtacctaa tttttcaaga
     3901 tatttctgtt gtatttgatg tatttgctct atagttttac ttgcgctctt atcattaaat
     3961 atatttaatt ctactagatt ttctctatgc tttgtaatcc caactggaac tatcgcaaca
     4021 ctgtttacat atggataaag ttgagctagt tcttttattg ttctatccaa ttcctttcca
     4081 tcatttatac ctggacataa aacaatttga cagttcattt ctatatgagc atctgccaat
     4141 ctcttcatta taccatataa ttttcctgca aatttattgc ttatcatttt ttgtcttaac
     4201 tctggattag ttgtatgcac agatatattt ataggactta ttctatattt tataatatta
     4261 tttatatctt cttcactcat atttgtaagt gtgacaaaat ttccttgtaa aaaagataat
     4321 ctcgaatcat catctttaaa ataaagagtt tctctcatac cttctggcaa ttggtctata
     4381 aaacaaaaca tacacttatt tctacagctt ttagcttggt caattatcgg atttgtaaat
     4441 tcaacaccca aatcttcatc atagtctttt tctatctcgt atatgtagac ttcgccatct
     4501 tttttttgta tttctacatc taaatattca tcacttagta agaatctata ctctattata
     4561 tcatgtattg gctgttcatt cacagatata agtaaatctc ctacctctat acctatttct
     4621 tctgcaatac tatctttata aaccttactt ataatattat ttactttttt tacatttact
     4681 tccaatattt tcaccactct tttgtattca tcaatgctta ttataatata ttttaatatg
     4741 caatttattt atttattata ttttatgata gcatttttaa gcatttcatg tcataataac
     4801 acattttaat tctaatagtc aatataagta aatagctata aaatagatta gaacaaataa
     4861 tattcaaatt taagtttctt aaaaatttga atattatttg ttcttaaaaa aattttataa
     4921 atttaatgtt ttactatgat taacgtccca tttgtcatat catgaaccat tccacctaca
     4981 cactttgaca tcaacaatgc cttattttgt aagtctttct catcatttgc ataaaatatt
     5041 ggacaacctc caccattttt catattctta tcaagtgtaa ctatagctag ggtatattca
     5101 tttaaaccta tatccattcc catcttatgc acttcctttc tttcctattt tataattttt
     5161 tagtgataag ttttttcctt tagagcttga aagtattggg catgacttta cagcttccat
     5221 tactttttcc atattcttat ctactggtaa ataagcaatc attatacttt catcctcttt
     5281 atttcttcta ggtaatggta cgaatgaagg ttctccataa tctccatata aacctaacct
     5341 tgaatatata ttgtatacta ttgcttgtct ttgaccggag tcaaataata ttcctgcatt
     5401 agcataatcc ttatttttag gaattatttg tattcctaca cctctattaa tatatacttc
     5461 tctatcactt tttagaccta tatttgttat accttttaaa tctcccacct ttagtataga
     5521 ttcgtccaca aaactaattt cagcaggcat tacatcagct atgtctccaa tactttttct
     5581 agtaagaagt ttttttaata taaaggctaa cactaatcca caaattgaac ttattaccaa
     5641 actcatccct tcacttattt tcgtttctgc aagtattaca taatacaatc ctattgttaa
     5701 aaaagatgta actatacaca tataatttct tacttcatag gttctagcta tctcttctat
     5761 gaatgcagca cctctcttta caagttgaac ttcctctaaa ttttgcaatg tatttcttct
     5821 atttgctctc acttgtctaa attgttctgc tgctagagat aaaaatgtta tggatgtata
     5881 agaacgattt ataagagctg gaaccaataa tgctcctaat gaagaagcga cgaatgctaa
     5941 tataagttga gatagtaaaa catttggttg cgttggatac tgtttttggt ctaaattcaa
     6001 cattataatt ctagatgtta taccaataat aacagcaaca aaaaatactc tatccattaa
     6061 atcactccta aaatatttaa tttgaaattt tacatgtaaa tctacaaagt ctttctagaa
     6121 aaatcactct gctttccttt tgatgtttct atgataggtg tacttttcac tacatctatc
     6181 atgacatcta tatctttgag tattggcata tatggtataa ccacagtact tttttctaaa
     6241 ttggtcttag atatagcaac aatatcaacc tcatctacat ctctatctat ccccatatga
     6301 ataaatagat tatgtattat agcttgtctt tgccctatat catttactat tccaaatgca
     6361 cctaaatctc ttggtataac ttctatggcc agacattcat ttctatactt ttctcttgta
     6421 ctttctagac ctatattagt tattattaca tcatttacca ttaaaatagg tccttcaaaa
     6481 cttatttttg caggaactat atctgctata tctgctatac tgtttcttct taaaaatctt
     6541 ctaaaaatca agccaacaat agctcctgac acaatagcta aaattgtgca atgaaaaaag
     6601 ctaaggtcat actttcttgc aaaaacgata taaactatcg aagatactag tgcagaaaaa
     6661 agacttatat aacttctaga ttcatacgta gatgctattt cttcaacata agcatctcct
     6721 tttggtacta attcttcatt atcaatattc tttagtgtta ttctttcttg ttccgcaaga
     6781 ccttgaaact gttgtattgc tactgcaaaa aatgtcaagg ctgcaaactc cttatctata
     6841 agtgcaggta atgctatagc acctaacgaa gctgaaagcc cagatataat tatctgttct
     6901 aaataatctt gtggtcttga tgggtattgt ttgtctgtaa ctcttaaaac taatcctcta
     6961 cataatattc ctacaactaa tgcaactata aaagagtgtc taaataaatt tgcattcaat
     7021 aaactttctt ccattttctc agctccttct cttatatagt ttgtgcaaaa aacaaaaaaa
     7081 attcccttct atttaaggaa atttttttgt aatattgtaa ctataaattc gctaccaatg
     7141 ccaagttcac tttttacttt tatacatcca tttagtgatt tgactatatg ctttgttata
     7201 gccaatccta aacccgttcc tcctacatcc ctacttcttg ctttatctac cctataaaat
     7261 cgttcaaata ttcgttctac atcttctttt ggtatcccta caccattgtc tataacttta
     7321 attacgattt tttcttcatc ttttaacact acaacttgta caatcccatt atcttgggta
     7381 tactttatag cattatcaac taggtttaag aacacttgct ttatatagtc tttattggat
     7441 agtatactta ttgatttatc atcacattta tataaaactt ctatattttt ttcactagct
     7501 gaatgttgag ttatttcata tacctcttta aatacatcat atactacaac attttccata
     7561 agaagagtat ctttattttc tacaaatgat aacagcaata tatcatcaat caaccttttt
     7621 aatctatctg attcgctttc tattatttct aagaatctat ttctagtatt cacatctata
     7681 ttttcatttg actttagtgt ttcaacaaac ccacttatag atgtaagtgg tgttttaagt
     7741 tcatgactta catttgcaac aaaatcactt ctcatatttt caagtttaac ttttttagta
     7801 atatcttcta tatttactat cgaacctatt attacgttgt tagtattctg aagataaact
     7861 gggtctacct ttatcttata tacgatatca tctttagtag taatattagc agtttcatta
     7921 tctttagaac caataaattg aagtatttgt tttaatattt cttcttcttt tattacaaca
     7981 ttaatattat gaccttcaac taattgattt ggttcactct ttatcatttc ttttgcttca
     8041 tcatttatca gaagtatatt tccatttaca tctatagcca aaattccatg agatatactc
     8101 tttaatatag atcttaactg taaatgttta tactgtactt cttctaaggt tttatccata
     8161 ttctttatca tatgattaaa atttctagct aattcaccaa gttctccttt ggctgatatg
     8221 ttaagcctag catgatattc attattactt actttttttg agacgtatat aaactctttt
     8281 aaatatttcc ttaatgttat tgtatatctt acagctaata taacaatggc tatggacaac
     8341 ataacaataa aaaaatatat actatttaaa tcttccacaa gataacactc ctaaaatata
     8401 acctttttaa tctattttat accctacacc tcttatagtc tctatatatt tttccgattt
     8461 atcttcatct tctatttttt ttcttaaata tcttatatga acatctactg ttctagtttc
     8521 tccatagtat tcataccccc atattttatc caaaagataa tttcttgaaa gtacttttcc
     8581 tttattttga attaataatt ttaacaattc aaattcctta agagttaact ctatcttttc
     8641 agagcctttg gtaacttcat gttttgataa atctagtttt aaattgcctg tagtaagtat
     8701 attgttttct tcacctaagc tagtcaaatt atatctcctt aaaacagaac taatcctagc
     8761 taacaactct tttatactaa atggcttagt tatatagtca tcagcaccta tttcaagccc
     8821 ttcaaccttg tcattttcca tatttttagc agtaagcatt ataacaggaa tattttttaa
     8881 gtccttatca cttcttattt ttctcaaaac ttctattcca cttatatttg gtaacatcca
     8941 atctaaaagt attaaatctg gtttaatttc cttagccttt ataaatccgt caaatccatc
     9001 atatgaataa ctaacttcgt aatttgacac ctccaaattg aattttaata gctcaactat
     9061 atgcatttca tcgtctataa caagcacttt agtattcatt aaaaagctcc tctctatttt
     9121 atctgtagta ccgttcctat tcgttctgat gccttattat gttttctata agagtggaat
     9181 ttatctgctc tacaactagt gcataaattt aaatttatta tattttcttt tttaactcca
     9241 ctacatctaa gcatatactc attaattttc cataaatcta aatagtaact tccctctttt
     9301 attatataga acttttcatc attatttgta agaattgtgt taaatttttc tactaaatct
     9361 tcagacactt catagcaaca tggtccaatt gatggcccaa ttatacatac taaatcttca
     9421 ggaactgttt tatagcattc agacattaat tttattgttt tatgacctat attactaaaa
     9481 gtccctctcc aacctgcatg agccagtcct atggctttat ttttagcatc tataattgat
     9541 ataggtacac agtctgctgt aaacagtaaa agtggtactt tttccaagtt tgttataagt
     9601 gcatctccat ctcttctttt ccctatagta tctttattga ctatattaac tatatctgaa
     9661 tgtatttgag aattactagt tatattttca aatgtcaaat cacattcttt acatactttt
     9721 aacatatctt ctctattttt tgcatctata ttcttacatg ttataactag atttacagaa
     9781 tttaattttt catcaagttc ttcacaatta ccacttaaat taacatactc tttaatatct
     9841 tgtacatttt ttatatttat ataatctttc atcatttgtc tcctttttct atcagtatac
     9901 ttttaagttc atttacaaaa gtatttatat ctttaaattg tctatacaca gaagcaaatc
     9961 ttacataggc aacctcatct aactctttca atttatccat taccatctca ccgatttttt
    10021 tggtttcaat ttcttctata aaacatttat ttatttcatt ttcaatatgt acaacaatac
    10081 tttcaatctc ttcaatagaa acaggtcttt tttcacatgc ttttagcaat ccatatttta
    10141 tcttttctct atcaaaatat tctctactat tatccttttt aatgaccatt aaaggagttg
    10201 tttctatttt ctcatacgta gtaaacctct ttttacatga ttcacactca cgtcttcttc
    10261 tgatagattt taaatctgta tgtctagaat ctattacctt tgattcttta taattacaat
    10321 aaggacattg catctatatt cctcctttgt ttaactatat ctcaaactca tctaagctta
    10381 attctgaacc tatatttact attattgtat cacaacctat tttaagtata tcattccaaa
    10441 atataacttc ttcatcccct cctcttgaaa aaaaacttct acttccatct ccaaaaatgg
    10501 taaatgagac taccttacct tcgtgaatat ccatgtctat gtctattata aaccccatcc
    10561 tttttccatt ttttacattt ataatttctt tttccattat atcagaaact ctaatcatat
    10621 tgtactcacc ctttctttaa aaataaatat gtatcgaagt aaaaaaaaat gacctatata
    10681 ggtcatttaa attaaaataa tgtttacagt ccatggatta cttatacata ttttctcata
    10741 ttttttaaag catttttttc aattcttgat acttgtgctt gagatatacc gatttcatca
    10801 gcaacttcta tctgagttct tcctttgtaa aaccttaaat ctaataccaa cttttctcta
    10861 ctatttaatt tcttaatagc ttctttaagt gatatttctt gcaaccaatt ttcatcagta
    10921 tcttttttat cttgaacctg atccataaca aatattgcat ctccattgtc ttgatatact
    10981 gggtcaaaca gagatattgg gtcttgtatg gcatcaagtg ccattacaac tgattctact
    11041 tctaattcta attcctttgc tatttctgat actgtaggtt ctttagaatt tgttctaatt
    11101 aatctttctc taacttgtag tgccttatat gctatatctt ttaatgacct acttactcta
    11161 attgggttat tgtctcttag atatcttcta atttctccta taatcatagg aacagcataa
    11221 gttgaaaacc ttacattttg gcttaaatca aagttatcta tagcttttat aagaccaata
    11281 caacctattt gaaataaatc gtctatattt tctcctctgt tgttaaactt ttttattaca
    11341 ctcaagacta atcttaaatt tcctcttaca aactgttgtc tggcttcttc atcaccattt
    11401 tttatttgta ataaaagttc cttcatttgc ttatttttaa gaactggaag ttctgatgta
    11461 ttaacaccac atatttcaac cttattaact tgcatatatt ttcagtcctc tctcaaaagt
    11521 attcatctat tgaaattatt tacatttcca atttttttat actttttaag aagaattaat
    11581 ttaacattat acaaattttt tcatttcttt ttgcaacctt gagattattt tcttttcaag
    11641 tctagatatg taggactgtg atattccaag cattccagca acttcttttt gtgttttttc
    11701 aataccttta tcaataagac caaatcttaa ttccataatt tgcttttctc tgtcacttaa
    11761 tctgtcaagt gccataacca acaaatctct atctatttct tcttcaatta ttttgtatat
    11821 ttcatcattt tctgtaccca atacatcaga taataaaagt tcatttccat ctaaatctat
    11881 atttaaaggt tcatcaaatg atacttctgt tttcttttta ttattttttc ttaagtacat
    11941 taatatttca ttttctatac atctcgaagc ataagttgcc aattttatat ttttattcag
    12001 tttaaaagta ttaactgctt ttatcaaacc tatagttcct attgaaatca aatcttctac
    12061 atctatacct gtattttcaa attttctcga tatgtagact actaatctaa gatttctttc
    12121 tattaaaatt gactttacac tttcatctgt ttctagcttt tgtaaaagct ccatttcttc
    12181 ttctggtttc aatggaggag gaagtatatt tgcccctccc atatagtata ttccctttgg
    12241 ctttattatt tttaatttaa tacctaacat agtaataaag cttattattc tttctttcaa
    12301 tcgtaacatt ccttgtcccc cttaaatttt cacctaaaat ccaggcaata tatttggatt
    12361 cattattgct ccatattctc tatcattaaa atttgaaata ccaagtatta tatttcctat
    12421 cttttgctca tctacttcta tatagtcagc ttttaagcct attattatgc ttgtcttatt
    12481 acttccagca tgtttataag gtattattct aactctacct gaagttttat catctaaact
    12541 tcctataatt tcttgtgctt tcttcatatc tatatcttca taattaatat ttaatagttt
    12601 ttctggaata aatccttcta acacactaga cttaactatt attacatcac tttgacttat
    12661 tgggtctttt aacaaattac cactatctac taaagcttta
//
LOCUS       DAEPOZ010000114        12655 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.114, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000114 DAEPOZ010000000
VERSION     DAEPOZ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12655
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.114"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            180..2525
                     /gene="secA"
                     /locus_tag="KR440_002279"
     CDS             180..2525
                     /gene="secA"
                     /locus_tag="KR440_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBH0699323.1"
                     /translation="MSVLDAILDKADEQEIKKLNLIVDKIESLEKDMEIKENEQLKEM
                     TNTFRLRLDKGESLDDILPEAFAVVREVSKRVLGMRQYKVQLIGGIVIHQGKIAEMKT
                     GEGKTLVEVAPVYLNALTGKGVHVITVNDYLAERDKELMRPVYEFLGLTVGVILSNQD
                     PSIRKEQYKCDITYGTNSEFGFDYLRDNMVPDLSHKVQRELNFAIVDEIDSILIDEAR
                     TPLIIAGDGDEDLKLYELANNFVKTVKEEDFEMDRKDKTIALTASGISKAESFFGITN
                     LTDIKNIELYHHINQALRGHKLMEKDVDYVISNGEVMIVDEFTGRVMDGRRYTDGLHQ
                     AIEAKEGVEIKNESKTMATVTYQNFFRLYEKLSGMTGTAKTEEGEFESIYKLNVVQIP
                     TNRPVIRADLHDKVFKTEEEKYSAVVEEIIRIHKTRQPILVGTVSVEKSEKLSKMLKK
                     QGIKHQVLNAKQHDKEAEVISKAGKLDAITIATNMAGRGTDISLGAGDKEEEEKVKNL
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDDVIKLYGGKTIEKLMKR
                     TSSKENTAIESKALTRAIERAQKGVEGKNFEIRKNVLKYDDTINEQRKVIYNERNKVL
                     NDEDIKEDIQKMVKDIIQEAGETYLIGRKRDYYGYFKHLYSTFMPADTLLIPGVDKKS
                     VQEIIDSTYEISKRVYDLKKMMIGIDKVAELEKTVLLKVVDQYWIDHIDAMEQLRQYI
                     GLKSYAQKDPFKEYALEGYDMFEALNKNIREATVQYLYKFN"
     gene            2940..4811
                     /locus_tag="KR440_002280"
     CDS             2940..4811
                     /locus_tag="KR440_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp2"
                     /protein_id="HBH0699324.1"
                     /translation="MNKKNLSVIMAAAMISTSVAPVFAAETTQVKKETITKKEATELV
                     SKVRDLMSQKYTGGSQVGQPIYEIKVGETLSKLKIITNIDELEKLVNALGENKELIVT
                     ITDKGHITNSANEVVAEATEKYENSADLSAEANSITEKAKTETNGIYKVADVKASYDS
                     AKDKLVITLRDKTDTVTSKTIEIGIGDEKIDLTANPVDSTGTNLDPSTEGFRVNKIVK
                     LGVAGAKNIDDVQLAEITIKNSDLNTVSPQDLYDGYRLTVKGNMVANGTSKSISDISS
                     KDSETGKYKFTIKYTDASGKAIELTVESTNEKDLKDAKAALEGNSKVKLIAGDDRYAT
                     AVAIAKQTKYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKDI
                     IKKSPSAKIYIVGGESAVSNTAKKQLESVTKNVERLAGDDRHMTSVAVAKAMGSFKDA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLSADAIKLMDGKEIGIVGGSNNVSSQ
                     IENQLADVDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKADITDSQRNALSKKLNLGAEVTQIGNGVELTVIQKIAKILGW"
     gene            4918..5625
                     /locus_tag="KR440_002281"
     CDS             4918..5625
                     /locus_tag="KR440_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBH0699325.1"
                     /translation="MVDATLFFTPHQDDETLSMGSAIIEHVEKSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESEFSKDRDEEFKDSCEAMGVKESNIHIEDNRAH
                     DGELSKEKAREIILKYLEEYPDAKVKTVTPFKASGIHEDHRALGEAALELYKEGKIKD
                     LRFYVEPYDYKDFKKVNPNVEVWKVLPSQEEKLLSAMNAYKKWNPESGHYAIGYHSVK
                     SHFDELAINKIQYVHAP"
     gene            5658..7493
                     /locus_tag="KR440_002282"
     CDS             5658..7493
                     /locus_tag="KR440_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBH0699326.1"
                     /translation="MKISKKIVSLLTMTFLTVTLYGNTSNASTKDTLTGSGRWETAIK
                     ISQAGWKKSENAVLVNDNSIADALSATPFAKAKDAPILLTQSNKLDSRTKAELKRLGV
                     KNVYLIGGSIALSSEIEKQLNAENISFERISGNSRYDTSLKLAEKLDREKSISKIVVV
                     NGEKGLADAVSVGAIAAQENMPIILSDSENGTEVADNFIDSKDIEKSYVIGGTYSISN
                     SVERSLPNATRIAGSSRSETNAKIIEEFYKDTDIKNIYVTKDGTRSKHDLIDSLAVGV
                     LASKNGSPILLAGNKLDSSQKDVLNTKIIDKVIQIGGLGNENVVEDVIDMQEETKYTV
                     ETIDELNVAIKKSDANDIIRFKPEKDKNITNSFKIETNKAITIEFDGIYKQSITIDMP
                     NGDIKNFGDIDNDLVINNIKNETLVNEGSIQGIDVYDENGCKIENEDSGEIWLITILK
                     NSKDVYILNRGEITKISNNSSSVTIKNSGNINTISGSEEPSISGKKPITNDTDKDEDK
                     KARGLYPKVESCIPALKDYVRITISTKPESSKYKIYYRVVDEKPSAKYVGEKISTKGW
                     SMVTISNQSFIERAKDGSYIEAVEIDTSNNEVSRWGKSSITDDGI"
     gene            complement(7541..7936)
                     /locus_tag="KR440_002283"
     CDS             complement(7541..7936)
                     /locus_tag="KR440_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBH0699327.1"
                     /translation="MILKKHRETILYLFFGAFTTLVNIVSYLFFTRVILFNFMVANAL
                     AWILAVLFAYVTNKFFVFESKRIEIRFVFKEFLSFVSFRLLSGVVEMLIMYVMIDLLF
                     VNDVIVKIFTNIVVIVLNYLFSKMIIFKK"
     gene            8163..10574
                     /locus_tag="KR440_002284"
     CDS             8163..10574
                     /locus_tag="KR440_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp84"
                     /protein_id="HBH0699328.1"
                     /translation="MRKYKSKKLSKLLALLTVCFLIVSTIPVSAENHKTLDGVETAEY
                     SESYLQYLEDVKNGDTAKYNGVIPFPHEMEGTTLRNKGRSSLPSAYKSSVAYNPMDLG
                     LTTPAKNQGSLNTCWSFSGMSTLEAYLKLKGYGTYDLSEEHLRWWATGGKYGWNLDDM
                     SGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATKPSNMDTAPTQFNVTDVVRLNK
                     DKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTNKRAPLNHAVAIVGWDDNYSKD
                     NFASDVKPESNGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETD
                     IDGYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISIGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNESAFNELN
                     TLLGK"
     gene            10871..12448
                     /locus_tag="KR440_002285"
     CDS             10871..12448
                     /locus_tag="KR440_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HBH0699329.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSSNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKGNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVENLEAKVDSIEKAIDSISQKVN
                     KIQDILDKIGFLKKFLS"
BASE COUNT         5270 a         1413 c         2293 g         3679 t
ORIGIN      
        1 tttcgaatac aaaaaaagag actaaattta gtctcttttt tatgtgagaa atacttaaat
       61 atataaagat gtaatttatt taagtatgat tattatacta tacaataaaa taattgtcta
      121 ctattctata attttaaggt ataataataa ttgtatgata aaagaggagg tcaaaaaata
      181 tgtcagtttt agatgcaatc ttagataaag cggatgaaca agaaattaaa aaattaaatt
      241 taatagtaga taaaatagaa tctttagaaa aagatatgga aatcaaagag aatgaacaat
      301 taaaagaaat gacaaatact tttagactta gattagacaa aggtgaaagt ttggatgata
      361 tacttccaga agcatttgct gttgttagag aggtctcaaa aagagtttta ggaatgcgtc
      421 aatataaagt tcagttaatt ggaggtatag taattcatca aggaaagata gctgagatga
      481 agactggtga ggggaaaact ttagttgagg tagctccagt atatctaaat gctttaacag
      541 gaaaaggtgt acatgtaatt acagtaaatg attatttggc agaacgtgat aaagaactta
      601 tgcgtccagt atatgaattt ttaggattga ctgtaggtgt aattttatct aaccaagacc
      661 caagtataag aaaagaacaa tataaatgtg atataactta tggaacaaat agtgaatttg
      721 gatttgacta tttaagagat aatatggtgc cagatttgtc tcataaagta caaagggaac
      781 taaactttgc catagtagat gagatagact caatattaat agatgaagct agaactccac
      841 ttattattgc aggagatggt gatgaagatt taaaacttta tgagttagca aataactttg
      901 taaaaactgt taaagaagaa gactttgaga tggatagaaa agataaaact atagcattaa
      961 cagcaagtgg tataagcaaa gctgagtcat tttttggtat aacaaacctt actgatataa
     1021 agaatataga attatatcat catataaatc aagctttaag aggtcataag cttatggaaa
     1081 aagatgttga ctatgttatc tcaaatggag aagtaatgat agttgacgaa tttacaggaa
     1141 gagtaatgga tggtagaaga tatacagatg gacttcacca agctatagaa gccaaagaag
     1201 gtgttgagat aaagaatgaa tctaaaacta tggctactgt gacttatcaa aatttcttca
     1261 gactatatga aaaactttct ggtatgactg gtactgcaaa gacagaagaa ggggaatttg
     1321 agtcaatcta taaattaaat gttgtccaaa taccaactaa tagaccagtt attagagctg
     1381 atttgcatga taaggtattt aaaacagaag aagaaaagta tagtgctgtt gtagaagaaa
     1441 taataaggat acataagact agacagccaa tactcgtggg aacagtttct gttgaaaaat
     1501 ctgagaagtt atctaaaatg cttaagaagc aaggtattaa gcatcaagtc ttaaatgcta
     1561 aacaacatga taaagaggca gaggtaattt ctaaagctgg taaattagat gctataacga
     1621 ttgctacaaa tatggcaggt agaggtacgg atatttctct aggtgcagga gataaagaag
     1681 aagaagaaaa agttaaaaat ttaggtggtc tttatgttat aggaacagaa agacacgaat
     1741 caagaagaat tgacaatcag cttagagggc gttctggtcg tcaaggagac ccaggtacat
     1801 caagattttt tgtaagtctt gaagatgatg taataaagct ttatggtgga aaaactatag
     1861 agaaacttat gaagagaaca agttcaaagg aaaatacggc tattgaaagt aaagcactta
     1921 caagggctat agaaagagca caaaaaggtg tagaaggtaa gaattttgaa ataagaaaaa
     1981 atgttcttaa atatgatgat actattaatg agcaaagaaa ggttatatat aatgaaagaa
     2041 ataaagtgtt aaatgatgaa gatataaaag aagatattca aaaaatggtt aaagacatca
     2101 tacaagaagc aggagaaact tatttaattg gaagaaaaag agattattat ggatatttta
     2161 aacatttata tagtacattt atgcctgcag atacattact aatacccggt gtagataaaa
     2221 aaagtgtcca agagataatt gatagcacat atgaaatttc aaaaagggtt tatgacttga
     2281 aaaagatgat gattggtatt gataaagttg cggagttaga aaaaacagta cttttaaaag
     2341 tggttgatca atactggata gatcatatag atgctatgga acagttaaga cagtatatag
     2401 gtcttaaatc ttatgctcaa aaagatccgt ttaaagaata tgctttagaa ggatatgata
     2461 tgtttgaagc tttaaataaa aatataaggg aagcaacagt acaatactta tataaattta
     2521 actaaaaaag ttcatgtaat ttttattaaa tgaaaaataa aatattttga ttctttatat
     2581 tgtaattaca attaaataat taaggaatac aaatgaaaaa gaagctgaaa atcagcttct
     2641 ttttctgtgt gagaaatacg gaaaaaagag atgcttgaaa tttctttctt ccatttaata
     2701 ttatactata aagaagatga aaagtctatt agttataaac tggattatta aaaaaaatta
     2761 caattttgtt acttttttgt atacataaat attaaaaaaa taaaatataa gaaaaagttt
     2821 atatcttttg gttaattatt acaataagtc tcatttattg aaataatatc aaatatatat
     2881 tataattggt aaataaggaa aaaataataa aaatttgaat tttttagggg gaaaatacca
     2941 tgaataaaaa aaatctttct gtaattatgg ctgctgcaat gataagtaca tcagtagctc
     3001 cagtttttgc tgcagaaact acacaggtaa aaaaagaaac aataactaag aaagaagcta
     3061 cagaactagt ttcgaaagtt agagatttaa tgtctcaaaa gtatactggt ggttctcaag
     3121 ttggacaacc aatatatgaa ataaaagttg gcgagacttt atcaaaatta aaaataataa
     3181 ctaatataga tgaattagag aaattagtaa atgctttggg agaaaataaa gaacttattg
     3241 taactataac agataaaggg catataacaa atagtgcaaa tgaagtagtt gcagaagcaa
     3301 ctgaaaaata tgaaaattca gcagaccttt ccgctgaggc taattctata acagaaaaag
     3361 ctaaaactga aactaatgga atttataaag ttgcagatgt aaaagcttca tatgatagtg
     3421 ctaaagataa gttagttata actttaagag ataaaacaga cacagtaact tctaaaacta
     3481 tagagatagg tattggtgat gaaaaaattg atttaacagc aaatccagtt gattcaacgg
     3541 gaacaaactt agacccttct acagaaggat ttagagtaaa taaaatcgtt aaactaggtg
     3601 tagcaggagc taaaaatatt gatgatgtcc aattagctga aataactata aaaaatagtg
     3661 acctaaatac agtttcacca caagatttat atgatggata tagattaact gttaaaggta
     3721 atatggtagc aaatggaaca tcaaagtcaa ttagtgatat ttcatcaaaa gattcagaaa
     3781 caggaaagta taaatttact attaagtata ctgatgcatc tggaaaagca atagagctta
     3841 ctgtagaaag tactaatgaa aaagatttaa aagatgccaa agctgcatta gaaggtaatt
     3901 caaaggttaa attgatagct ggagatgata gatatgcaac tgcagtggct atagcaaaac
     3961 aaacaaaata tactgacaat atagttatag ttaattcaaa taaactagtt gatggattag
     4021 cagctacacc acttgctcaa tctaaaaaag cacctatatt attagcatcc gataatgaaa
     4081 taccaaaagt aactttagat tatataaaag atataattaa gaaaagccca tcagctaaaa
     4141 tatatatagt aggtggagaa tcagcagtat caaatacagc taaaaagcaa ttagaatcag
     4201 taactaagaa tgttgaaaga ctagctggag atgatagaca tatgacttct gtagcagtag
     4261 caaaagctat ggggtctttt aaagatgcat ttgtagtagg tgcgaaaggg gaggctgatg
     4321 ctatgagtat agctgccaaa gctgctgaac ttaaggctcc tataatagta aatggctgga
     4381 atgatctttc agcagacgct atcaaattga tggatggaaa agagattggt atagttggtg
     4441 gttctaacaa tgtatctagt caaattgaaa atcaacttgc tgatgttgat aaagatagaa
     4501 aagttcaaag agttgaagga gaaacaagac acgatactaa tgctaaggtt atagaaacat
     4561 attatggaaa attagataaa ctatatatag caaaagatgg atatggaaat aatggtatgc
     4621 tagtagatgc attggcagca ggacctctag cagcaggtaa aggtccaata cttctagcta
     4681 aagctgatat aacagactca caaaggaatg cacttagtaa aaaattaaat cttggtgcag
     4741 aagtaactca aataggtaat ggagttgaat tgacagtaat acaaaagata gctaaaatac
     4801 taggttggta ataacttata ctgtggagaa gattccttaa ttaatacagg aatctttttc
     4861 tataattatg ctatttattg taaattataa cattttataa agtaggagga tgtagtagtg
     4921 gtagatgcaa ctttattttt tacaccacat caagatgatg aaaccttaag tatgggaagt
     4981 gctataattg aacatgttga aaaaagtgat acacatgtca tattatgcac agatggtagt
     5041 aaatctataa taagaaaagt tttggatgat ggagggtcat gttcttatca tattaaagat
     5101 gttcataaat actctttatc agaaagtgaa ttttcaaaag atagagatga agaatttaag
     5161 gatagctgtg aagcaatggg tgtaaaagaa agtaatatac atatagaaga taatagagca
     5221 catgatggtg agttaagtaa agaaaaggca agggaaataa tacttaagta cttagaagaa
     5281 tatccagatg caaaagtaaa aactgttact ccgtttaaag ctagtggaat acatgaagac
     5341 catagggcat taggagaagc tgcactagag ttatataaag aaggcaaaat aaaagattta
     5401 agattttatg tagaaccata cgactataag gattttaaaa aagttaatcc aaatgtagag
     5461 gtatggaagg tattacctag tcaagaggag aagttattaa gtgcaatgaa tgcatataaa
     5521 aaatggaatc ctgaaagtgg acattatgct ataggatatc attcagtaaa atctcatttt
     5581 gatgagctag ctataaataa aatacagtat gtacatgctc cataaaatat tatgaaagaa
     5641 ttaggaggta agaattaatg aaaatatcaa aaaagatagt gtctttgtta actatgacat
     5701 ttttaactgt tacattatat ggaaatacat ctaatgcatc tacaaaagat acattaacag
     5761 gttctggaag atgggaaaca gcaataaaaa taagtcaagc aggatggaaa aagtctgaaa
     5821 acgctgtgct agtaaatgat aattcaatag cagatgcttt atcagctact ccatttgcta
     5881 aagcaaaaga tgcaccaata ttattaactc aaagtaataa attggatagt agaacaaaag
     5941 cagaattaaa aagacttggt gtgaaaaatg tatatttaat aggtggttca attgcattaa
     6001 gttcagagat tgaaaagcag ttaaatgcag aaaatataag ttttgaaaga atatctggaa
     6061 atagtagata tgatacttct ttaaaattag ctgaaaagct agatagggaa aaatctatat
     6121 ctaaaatagt agtagtaaat ggagaaaagg gtcttgctga tgcagtgagt gttggagcta
     6181 tagctgctca agaaaacatg ccaataatac tttctgattc agagaatgga actgaagtag
     6241 ctgataattt tatagatagt aaagatatag aaaaatcgta tgtaataggt ggtacatatt
     6301 ctatttctaa ttctgtagaa agaagtttac caaatgcaac aagaatagca ggtagtagta
     6361 gaagtgagac aaatgcaaaa attatagaag aattttacaa agatactgat ataaaaaata
     6421 tttatgtaac aaaggatggt acaaggagta agcatgattt aatagattct ttagctgtgg
     6481 gtgtattagc ttctaaaaat ggttctccaa tacttttagc aggaaataag ctagatagtt
     6541 ctcaaaaaga tgtgttaaac actaaaatta tagataaagt tattcaaatt ggcggattgg
     6601 gtaatgaaaa tgttgtagaa gatgtaatag atatgcaaga agaaactaag tatactgttg
     6661 aaactattga tgaacttaat gttgctataa aaaaatcaga tgcaaatgac atcataagat
     6721 ttaagccaga aaaagataaa aatataacta attcttttaa aatagaaaca aataaagcta
     6781 taactattga atttgatggt atatataaac aatcaataac tatagatatg ccaaatggag
     6841 atataaaaaa ctttggggac atagataatg atctagtaat aaataatata aaaaatgaga
     6901 ctttagttaa cgaaggaagt atacaaggga tagatgttta tgatgagaat ggatgtaaaa
     6961 tagagaatga ggattctgga gaaatatggc ttataacgat acttaaaaat tcaaaagatg
     7021 tatatatttt gaatcgtgga gaaataacta aaatatctaa taattcttct agtgtaacaa
     7081 taaaaaattc aggtaatatt aatactataa gtggaagtga agaaccatca ataagtggta
     7141 aaaaacctat aactaatgat acagataaag atgaagataa aaaggcacgt ggtttatatc
     7201 ctaaagtaga atcttgtata cctgctttaa aagattatgt aagaataact atatcaacaa
     7261 aaccagagag ttcaaagtat aaaatttatt atagagtagt tgatgaaaaa ccatctgcga
     7321 aatatgttgg agaaaaaatt agtactaaag gttggagtat ggttacaata tcaaatcaat
     7381 cttttattga aagagccaaa gatggatcat atatagaagc agtagaaata gatacctcaa
     7441 ataatgaagt ttcacgatgg ggaaaatcta gcattacaga tgatggaatt tagacattaa
     7501 acttattcat tttgcagatt actatttagt aatctgctat ttatttttta aaaattatca
     7561 tcttgctaaa caagtaattt aaaactataa caactatatt agtaaatatt tttacaataa
     7621 catcatttac aaaaagcaaa tcaatcatta catacataat taacatttca acgacaccag
     7681 aaagtagcct aaaactaaca aaagacaaaa attctttaaa tacgaacctt atttcaattc
     7741 ttttactttc aaatacaaaa aacttattag taacataagc aaataaaaca gcaagtatcc
     7801 atgccaaagc atttgcaacc ataaaattaa ataaaataac tctagtaaaa aataaatacg
     7861 aaactatatt tacaagagta gtaaatgctc caaaaaataa atataatatt gtctctctat
     7921 gcttttttaa aatcaaatat aatcctccta atctaattta atataaatat acttaaagat
     7981 actaacaaac attagtatag cacatctatt aataaatcaa tatatcttaa aatattataa
     8041 aaataaaact ataatattct ataattaata tgtactcata atccatattt tatcaaaata
     8101 aaatatgacc ttaatttaga aatctgatat aataaaaatt aaatacatat aagggggtaa
     8161 acatgagaaa gtacaaatca aaaaaattgt caaagctact ggcattgttg acagtttgct
     8221 ttttaatagt gtcaacaata cctgtctcag cagaaaacca taaaactcta gatggagtag
     8281 aaactgcaga gtattctgaa agttatcttc aatacctaga agatgtcaaa aatggagaca
     8341 cagcaaaata taatggagta ataccttttc cacatgaaat ggaaggtaca acacttcgaa
     8401 ataagggcag aagtagtctt ccatcagcat ataaatcaag tgtagcatac aacccaatgg
     8461 atcttggtct tactacacca gcaaaaaatc aaggaagtct taatacatgc tggtcttttt
     8521 caggtatgtc aactttagaa gcatatctta aattaaaggg atatggaacg tatgacttat
     8581 cagaagaaca tttaagatgg tgggcaactg gtggaaaata tggatggaac ttagatgata
     8641 tgtcaggtag ttcaaatgta acagccatag gatatctgac agcatgggca ggtcctaagt
     8701 tagaaaaaga tataccatat aatcttaaat ctgaggcaca aggtgcaact aaaccttcaa
     8761 atatggatac tgcacctact caatttaatg taacagatgt tgttcgtctt aataaagata
     8821 aggaaactgt aaaaaatgct ataatgcaat atggttcagt gacatcaggt tatgcacatt
     8881 attcgacata ttttaataaa gatgaaacag catataactg tacaaataag agggctccat
     8941 taaatcacgc tgtagcgata gtaggatggg atgataatta ttcaaaagat aactttgcat
     9001 ctgatgtaaa accagaatca aatggagcat ggttagtaaa aagcagttgg ggagaattta
     9061 attctatgaa aggattcttc tggatttctt atgaagataa aactctacta acagatacag
     9121 ataactatgc aatgaaatca gtatcgaaac cagatagtga taaaaaaatg taccaacttg
     9181 aatatgctgg tcttagtaag ataatgtcaa ataaagtaac agcagcaaat gtatttgatt
     9241 ttagcagaga ttctgaaaaa cttgactctg ttatgtttga aacagactct gtaggagcaa
     9301 aatatgaagt atattatgca ccagtagtaa atggagttcc tcaaaacaac tcaatgacaa
     9361 aacttgcaag tggaacagta tcatattctg gatacataaa tgtacctact aattcttaca
     9421 gcttaccaaa aggtaaagga gcaatagtag tagttataga caacacagca aatcctaata
     9481 gagaaaaatc aactttagca tatgaaactg atatagatgg atactatcta tatgaggcta
     9541 aagcaaactt aggtgaaagc tacatacttc aaaacaataa gtttgaagat ataaatacat
     9601 atagtgaatt ttctccttgt aactttgtta taaaagctat aacaaaaaca tcttctggac
     9661 aagctacttc aggagaatct ttaactggag cagatagata tgaaacggca gttaaagtta
     9721 gtcaaaaagg atggacttct tcacaaaatg cagtattagt aaatggagac gcaatagtgg
     9781 atgctttaac agctacacca tttacagcag caatcgactc tccaattctt ttaacaggaa
     9841 aagataactt agattcaaaa actaaggcag agttacaaag attaggaact aaaaaagttt
     9901 atctaatagg tggagaaaat tctttaagca agaatgtaca aactcaactt agtaatatgg
     9961 gtatatcagt agaaagaatt tcaggtagtg atagatacaa gactagtata tctctagctc
    10021 aaaagttaaa tagtataaaa tctgtttcac aagttgcagt ggcaaatggt gtaaatggac
    10081 ttgcagatgc aataagtatt ggtgcagcag ctgctgataa caatatgcca ataatactta
    10141 ctaatgaaaa gagtgagttg caaggtgctg atgagttttt aaattcatca aaaataacta
    10201 agtcttatat aattggtggt acagctactt tatcatcaaa tttagaaagt aagctttcaa
    10261 atccaacaag acttgcagga agtaatagaa atgaaactaa tgctaagata atagataaat
    10321 tctaccctag ctcagatttg aaatatgctt ttgttgttaa agatggttca aagagtcaag
    10381 gagacttgat agatggacta gcagtaggag cattaggtgc taaaactgat tcaccagtag
    10441 ttctagttgg aaataagtta gatgaaagtc aaaaaaatgt acttaagtct aagaaaatag
    10501 aaactcctat tagagttggt ggaaatggaa atgaaagtgc ttttaatgaa ctaaatactc
    10561 ttttaggaaa atagtgaatg gaaagatagt aaggatatac attttattat taaaaaatat
    10621 tcaatagaag tttataaata taaaataaca aaaaggggtg tctcaaaatg aactttttag
    10681 ttcatgaggc actctttttt gttgttttat atttttatca tttaaataat gaaaaatagg
    10741 cttgtctata tttttagata gttttcttat tattgatgaa aaatgtaata attttaaact
    10801 gtaaaatagt gtaaagttat tttataatat aaatatagta agattttaaa caaaataagg
    10861 gggaataata atgaaagcac caaaaactat tttaacaata ctgacaatag cgcttacttt
    10921 aagtagcatt tctataatac catcatatgc acttacagag gaaaaattaa taggtaatgg
    10981 tagatatgaa actgctgtaa agataagtca aaaggcatac agttctagtt ctaatgtagt
    11041 gttagttaat gacaattcat tagctgatgc actttcagct acaccatttg caaaggctaa
    11101 aggagcacca atacttttga cagaaagtga taagctagat gataggactg aaaaagagat
    11161 aaaacgtcta ggagctaaag atatttattt gataggaggt actgctgtac ttaataaaga
    11221 tatagagaac aagttaaaag gtaatggact taatgtagaa agaattaatg gtaaggatag
    11281 atatgaaact tcactgattt tggctaataa gcttaaggat ataaaagata ttaaggaagt
    11341 tgctgtagta aatggtgaaa aaggattaag tgatgctgtt agtgttggag caccagcagc
    11401 tcaaaataag atgcctataa ttttgtctag tcctaaggac ggagtagaag catttgataa
    11461 atttataaga gatgagaaag ttataaaagc atacgtaatt ggaggaacaa attctgtttc
    11521 aagagctgta gaaaagagcc ttcctaatgc agaaagagtg agtggaaaag atagaaatga
    11581 gactaatgcc aaagttatag aaaagtttta tactgataca aatttaagta atttatatgt
    11641 aactaaggat ggtagtaaaa atgagaatca actaatagac tcgttagctg ttggagtatt
    11701 ggctgcaaaa aatgagtctc ctgttgtttt ggttggtaat aaattaaaca cgaaacaaag
    11761 ggatatactt agtactaaaa aattaaatac tataacacag gtaggtggaa atggaaatga
    11821 agaagctttt gatgaaatta agagtttaca agaaaaaact gtgtttgaag caaaaactgt
    11881 tgaggaatta actgatatga taaatatagc aagtcctaat gatattataa actttaaacc
    11941 aaaagagaat actgtaaatg aagcttttag aatggtaaca aataaaccaa taactgtgaa
    12001 tataaagggt gattgttcaa agactcttac agtagatatg cctaatggtg aagtgaataa
    12061 ctatgctact ttggtaaatg ttatagttag aaatattgga gaaggtggat ttaataacca
    12121 tgatacaatc actatattgt cagttcgtga caaaaatggt agagttatag aaaatactag
    12181 aaattcagat atagatacgt tgatgatatt agcttctgct aatgatacaa aattgattaa
    12241 tgatggatat ataggaaagc ttatagataa ttcatctaat agtgatataa ctaataatgg
    12301 tactatagat aaaaaagtta atcaagttga gaatttagag gctaaagtag attcaattga
    12361 aaaagctata gattctataa gtcaaaaagt caataaaata caagatatac tagataagat
    12421 tggatttcta aagaaatttc ttagttaaga atatgtatat ttaagtgaag tgtgtgttgt
    12481 aattgttcta aagttatata gttaaacagg tttatataca aacagactta tagataaata
    12541 tgtagtttag tatatatttg aataagcttt ggggaggtaa catggggatt ggaattgaat
    12601 gtaacaaaaa aataaataaa agtattgaca aaactagaat aaagaaaaat aatct
//
LOCUS       DAEPOZ010000115        12421 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.115, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000115 DAEPOZ010000000
VERSION     DAEPOZ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12421
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.115"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            186..389
                     /locus_tag="KR440_002286"
     CDS             186..389
                     /locus_tag="KR440_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0699330.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKGI"
     gene            553..1707
                     /locus_tag="KR440_002287"
     CDS             553..1707
                     /locus_tag="KR440_002287"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBH0699331.1"
                     /translation="MKKISILGSTGSIGKQTLDVVREHRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELVNITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFRVEQENIDVVVHPQSIIHSMVQYADSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            1720..2724
                     /gene="rseP"
                     /locus_tag="KR440_002288"
     CDS             1720..2724
                     /gene="rseP"
                     /locus_tag="KR440_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBH0699332.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSNKVVNVTPKNNNGKYEIGIQPQREKDFLASIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            2907..3962
                     /gene="ispG"
                     /locus_tag="KR440_002289"
                     /gene_synonym="gcpE"
     CDS             2907..3962
                     /gene="ispG"
                     /locus_tag="KR440_002289"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBH0699333.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSRERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKHGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            4310..5134
                     /locus_tag="KR440_002290"
     CDS             4310..5134
                     /locus_tag="KR440_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699334.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLASTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSIWKNSAYENWLKSFQGKEVSNSVLENYYK"
     gene            complement(5607..6730)
                     /locus_tag="KR440_002291"
                     /pseudo
     CDS             complement(5607..6730)
                     /locus_tag="KR440_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
     gene            6927..7151
                     /locus_tag="KR440_002292"
     CDS             6927..7151
                     /locus_tag="KR440_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699335.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            7204..7728
                     /locus_tag="KR440_002293"
     CDS             7204..7728
                     /locus_tag="KR440_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0699336.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(7833..7979)
                     /locus_tag="KR440_002294"
     CDS             complement(7833..7979)
                     /locus_tag="KR440_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699337.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(8118..9044)
                     /locus_tag="KR440_002295"
     CDS             complement(8118..9044)
                     /locus_tag="KR440_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBH0699338.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            9235..11106
                     /locus_tag="KR440_002296"
     CDS             9235..11106
                     /locus_tag="KR440_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBH0699339.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNASCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            complement(11198..12133)
                     /locus_tag="KR440_002297"
     CDS             complement(11198..12133)
                     /locus_tag="KR440_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBH0699340.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
BASE COUNT         4897 a         1299 c         2093 g         4132 t
ORIGIN      
        1 taaaataatg agataaatat atataaagct taatttttga acttatggtt ttgttttatt
       61 ttaacatatt tatatttaat aaactataat tgaaatctat ttagataaat aattatttat
      121 ctaaatagat gaaaattttc caaaatagat aaaatgtgat agaatatatt ttgcgaggtg
      181 ataacatgat ttcatacgct ccattatgga aattgttaat tgacagaaaa ataaagaaaa
      241 tggaatttgt aaatatatca ggtataagta tttctgtttt aggcagacta ggaaatgata
      301 aatctgtttc tatggataca atggagaaga tatgtttagc actagactgt aaaatagaag
      361 atgttgtgga aataaaaaag ggcatttaat aatactagaa tattttataa tattccacaa
      421 gatacaaaaa ataatagatt aattttaatc tgtggaataa tatatgaaat ttctatgaat
      481 agtaaaaata attgttttag tagtgttata atgttttgac agggtatatt tattattaga
      541 aaggattatt atatgaaaaa gatatcaatt ttaggttcta caggctctat aggtaaacaa
      601 actttggatg tggttagaga acatagagat aagtttgaga tagttgcaat atcagctaac
      661 agtaatattg aattattgct agaacaaata gtagagttta aacctaaata tgtaacagtt
      721 tttgaggaaa ataaggcatt gaaattaaaa gaaatgttgc ctaaaaatat agaaatagaa
      781 gttttagcag gaatggaagg attaaaaata atatcatcac ttgatgaagt ggatgtcctt
      841 ttaactgctg tagtaggtat gataggatta gtcccaactc tttgtgctat aaagaaggga
      901 atagacatag cattagcgaa taaagaaacg ttggtaactg ctggtgaact tgtaatgaaa
      961 gaagcagaaa aatacaatgt aaatattctt cctgttgata gtgaacatag tgctatattt
     1021 caatgtctaa atggagaaaa taaaaaaaat atagaaaaga taatacttac agcatctggt
     1081 ggtccattta gaggaaagaa aaagggagaa cttgtaaata taactaaaaa tgaagctttg
     1141 aagcatccaa attggagtat gggaagaaaa ataagtattg attcttcaac gcttatgaat
     1201 aaaggacttg aagtgataga agctagatgg ttgtttaggg tagaacaaga aaatatagat
     1261 gtagtagttc acccacaaag cataattcat tcaatggtgc agtatgcaga tagctcaata
     1321 attgctcaat tagggtgtcc agatatgaga ctaccaatac agtatgctct tacatatcca
     1381 gatagaatgg aaagtagctt tgaaagaatg aatttttcaa aatttagtac tttaactttt
     1441 gaagaaccag atttagaaac ttttccatgt ttaaaattgg cctatgagtg tttaaaaatg
     1501 ggaggaactt attcttctgt tttaaattca gcaaatgaag ttttagtaag tgaattttta
     1561 gaggataaaa ttggttttta tgacatacca tattacatag agaagacttt agaagttcat
     1621 agtagcataa gcgaaccaac attagaagaa atcttagaaa cagatagatg gagtagagct
     1681 tatgttgcaa atctgataaa aaaataggaa ggtgaattta tgactataat agccgcatta
     1741 atactattta gtataattgt attaatacat gagttaggac attttatatt tgcaaaaaga
     1801 agtggtatca aagtaaatga gttttctata ggtatggggc ctaagatata tagtgtaaaa
     1861 aaagatacag agtattctat aagagcactt ccaattggtg gatatgtcag catggaaggt
     1921 gaagatgaag aacaaataag tccaaattct tttggaaaca agtctatatt acaaagattt
     1981 agtactattg tagcaggacc aatattcaat ataatattag cagcaatact tttagttcca
     2041 gtatttttgt atataggttc tccaacgact aaattaggaa agataatgcc agatactcct
     2101 gcacaggctg tagggcttca agtaggagat aagataaata aaattaatgg taattctgtt
     2161 aagacttggg atgaagttgc aaatataata aacacttctt ctggtggaga attaaaactt
     2221 agtataacta gagatggaag taataaagtt gtaaatgtaa cacctaaaaa taacaatgga
     2281 aagtatgaaa tagggattca accacaaaga gaaaaagact ttttggcgtc aatagtaaat
     2341 gcatgtaaaa ctactgtaga tatgacaaaa cagatgttaa catttctagg tcaaatgatt
     2401 acaggacgtg taccaggagg gattggcaat gctgtagcag gaccagtagg tgttattggt
     2461 atggtatcag atgctgctcg tacaggcatc ataaatgtag tgtatttagc agctgtcata
     2521 agcttaaatt taggtatagt aaatctatta ccaataccag cacttgatgg atggagaata
     2581 cttatgctat tactagaagc tgttagaggt ggtaaaaaac tcgacccaaa caaagaaggc
     2641 atgataaatg ttgttggttt tggagcttta atgttattta tgctttttat cacatataaa
     2701 gatatattaa gattatttca atgatttagt tttttagaga ttttaagaaa ataggctatg
     2761 tagttttaaa taagaataaa ttaattttta tgtatgaaat ttattcttat ttaactagat
     2821 agctttttaa tttataatat acatgtaggt tataatattt tactatttaa agagattcaa
     2881 tataaaataa tatatagagg tggaatatga gttacgagag aagaaagacg agagaagtaa
     2941 gtgtaggaac tgtaaaaata ggaggagaaa atcctataag tatacaatct atgacaaata
     3001 ctgatacaag agatgcagat gctacaatag ctcaaataaa gagactagag gaagctggtt
     3061 gtgatatagt tagagttgct gttcctgata ttaaagctgc taagaatata gcaaagataa
     3121 agtctagtgt aaatatacca attattgcag atatacattt cgattataag ctagcccttg
     3181 aagctataga acaaggtgtg gatggaataa gaataaatcc tggtaatatt ggaagtagag
     3241 agagagttaa gatggtagtt gagaaatgta aggaaagaaa tttaaaaatt agaattggag
     3301 tcaatggagg ttctttagag aaagaacttt taaaaaagca tggctctgca actgctgaag
     3361 cattagttga aagtgcaatg ggacatatta agattcttga agacttagat tttcataata
     3421 tagttatatc tctaaaatca tccgatattt ataaaactgt tgatgcatat gaattaatat
     3481 caaaaaaagt tgattatcct cttcatattg gaattacaga atctggaagt gttcacaaag
     3541 gaacaataaa gtcttctata ggggtaggag cacttcttct taaaggaatt ggagatactg
     3601 ttagaatatc tttaacaggc gaccctgttg aagaggttat tgttggtaaa cagattttaa
     3661 gaagtttggg acttttaaat gataagatta aagttatatc atgccctaca tgtggtagat
     3721 gtaatataga cttaattagt gttgtaaatg aggttgaaga aaagattggt agtatggaaa
     3781 aaaatatcac tgttgcaatt atgggctgtg ctgtaaatgg acctggtgaa gccagagaag
     3841 cagatatagg tattgctggg ggtaaaggtg aaggtctttt atttaaaaag ggtgaaatag
     3901 ttagaatgat tgatggtaac aaattagttg atgagttgct agaagagatt gaaaaattat
     3961 agtatacaat tcatatatat attaattgac tagattttta aatacttttg taataagttt
     4021 ttatttacag tatttaatta tctagtcatt ttttatttta taaacttcat taaatgtaag
     4081 aaacttatat tctaatttga ctatatatgt atgagttaaa tcatattgta aatttataaa
     4141 attaaaaaaa gatttaatat agtgaaaatt tccatcaata gtattgaaac ttgatagtaa
     4201 ctatgatagg atatagttac tagaacagtt ggtaaattat gttaacatag attttaatgt
     4261 tttggtaaat tgttttatta taagttaata gaaaaagagg tgaagaactg tgagatataa
     4321 aaaaatatct agcttaccta ttttagttgt tgctttgctg attactggaa cgacaacaac
     4381 attttctctt agtacgacca attcagaaaa aacaaatgta tacttagcaa gtacggtaag
     4441 tactgtcaca aagaaagtat ccgatattca aagtagtaaa ggtcttgttt ataatgagaa
     4501 tattccatta tttataaata atagcaaaat acagtatgat gatactccat atcattggcc
     4561 tagcaatgtt ataagcttaa caaatagcag tgaaaaggca attatggatt atgagattac
     4621 atgcttagca tatgataaaa atggaaaacc acttgagcta tattgggatg ctcaaaatgt
     4681 ggcagcagat ggcgaagtag gaagtgttgg atttagccca gctggtgtag actatggtat
     4741 tgttacaggc atatctcctg tgtctccaaa atcatattca catacttata gaaaaatgca
     4801 acaatcacca ccacaggata taataagtat gtttgaaaaa caacaaggca aggcatgggt
     4861 agaaaattgg ttgaaagaat ggaaacaaat ggagaaagaa tatgcaaagc aaaatgcaat
     4921 agcaccaggt aagaatcaaa atgatgcctt tctattgttt gataaatgga aacaatcaac
     4981 tggagagcat ggtgtaaagt acattatatc ttgtgtaaaa caagtaacat ttaatgatgg
     5041 tagtatatgg aaaaattcag catatgagaa ttggttgaaa agttttcaag ggaaggaagt
     5101 ttcaaatagt gtattagaaa attactataa gtaatgttta atgatagcaa aatgagtacg
     5161 aatttaatta atgttaattt aattaaataa ggactatttt gtttaaaaat atcagtgtta
     5221 caaggaaatt tttattgtga caaaatggta tatattcaaa ataaaaaagc atgtgtcaag
     5281 ttttgataca tgctttttta ttttgaatat atgttttcta taaatttatg attttttagt
     5341 tatgtaattt tatatgtgaa tttttagatt tttatataga tagtgaaata atgtggaagt
     5401 tttatgtttt aaaaatttta gttaaaacat ttattaaaaa agataataga aatgttttaa
     5461 ctaaaatagt tagaattgaa atgttataca gatagtataa gtactatcaa attatataag
     5521 attgcttgaa gttaaataat ttatttaaat agaaaacagc tgatttaata tcagctgtat
     5581 atgaataaaa ttttgaattg tagtgtttag ttatatggat ataattcata ataagttagc
     5641 aataaatcaa ccattttacc ataactctga gtaccttcag taacgccatt agcttttaag
     5701 taagaattgt taaactcatt agaaatttca ttaattttac cttcatattt ttgccaaaat
     5761 tcactttcat tttttaaatc tctacggaca gaatctgaaa tgccagcaga aatatcaaca
     5821 tatgcattat aatcaacctt acttaaagca gaagcagtat aatttaaagc taaaatatat
     5881 ccagaatagt taaaatcaat atgggggtgt tttatagaag taagatacgc tataaaatta
     5941 gcctcatctt cactagcaaa tcccctttga tgtgctattt catgttcaac tgtacaagga
     6001 atatataagt caggtattgc tatatttaca tttgcctcac ctgtaaaagg aaagtatata
     6061 ccagttattc ctgtatagca cattagattt gatgaaataa tatacttagg cttagaataa
     6121 ctaccactaa cactaggtaa tatatccaag atattatcgt atcctagttg agctctattt
     6181 atgacaccat tataatctgt attagatttt acaataccat ttttatcttg taatgtgagt
     6241 tttctagttt catttgcttt aattacaagg aatttatata atttagtaag ctctttaaca
     6301 ctatattgtt ttgattggat agaggtattg ttttttaaat tatagttatt tataagtgtg
     6361 gtttctagtg gaatcctatt atagtttatt ccccaaagca ctacaaaaag aaagtagact
     6421 attgatacaa tggataatat atttaaaatg gaatttttta aaaatatgtt taattttttt
     6481 tcttattaaa aattgtagat aaagtacaac ataaaaataa gaagatagat ataactatta
     6541 tgtacattgt aatctcatag agagaaaatg ggaaaattcc agaaacttta ctcaatattt
     6601 gtacaattat tttatctatg aattgggagt aatatttttc aacaatattt ggaactttgc
     6661 tggctataaa atttaaaagt aaagcaatag gaaagagaat taaagaaaaa tattttgttt
     6721 ttttgctcat atttcacctc acatacatta gtttatataa acatattata tatcaattga
     6781 agaaagattg taaaataagt ataaatatat ctatttataa ctttatacta aataaatgat
     6841 aagttataat atattaatat gaataagcag tatatataca actaataata agaaaaatat
     6901 caattatata aggagtagat aaaaatatga taataaaact tactaaagac aattttaatt
     6961 ttagatttaa acaatatgaa ggtatgtttt ttcatgctga agtagttgaa ggtatggaat
     7021 ccattagata taaaataact aaggataaag aaggtacaga attaaaagaa tttggaagta
     7081 cacatataaa agataatatg atatatgtac ctcaggtaga agcatacata gatttgaaca
     7141 gtataaaata aagatgtaaa attttaatta aaaataaaga ttatgtttga gggggaatcc
     7201 aatatgagta agcctataga tataacaata agacctataa gaagagaaga tgcacaagct
     7261 tttaatgaaa ttagaagaac atacagtgtt atgagaaata ctttggcatt aatgagtgac
     7321 agagtcgata aaactgaagc tatgttagct tctttgggag aaaatgatta tatgtttgta
     7381 gctgaaacta ataataatgg agaaaaagat gtaataggtt ttgctgggtt acatgtaaat
     7441 tcttctccac gattgagaca tagtgcagaa ataggaataa gtgttgcaga gaattatcag
     7501 ggaagaggag taggtaagaa gcttatggaa caacttttag atatagcaga taattggatt
     7561 atgttaacta gagtaggatt agaagtaatt gtagataatg aaaagggatt aaatttatat
     7621 aaaaaactag gttttgaaat agagggaact aaaaaatatg ctgtaatcag agatggaaaa
     7681 tttgaagatg tttatattat gggaagatat aataaaaatt taatttaaag aagctaatag
     7741 agaagtaaaa tagagtgtct ttaataaaaa atttacatta aaaataatct attttacttc
     7801 tctattgtat taaaaaactt atttttaaat gtttacaaaa acattctata tacaaataaa
     7861 attattagca atatcaacgc tattttaaaa gctaaactag caacagtttt aatgattttt
     7921 acagaaacac ctatagcaac tatagcaaat actattttaa agagtaaatc catatccata
     7981 tttaagcctc ctattctata atacatatag atattattga caaatatcta ggaaaaaatt
     8041 ccatcaatat actacaataa aatgtttata tttgtaattt attgaatatg ttattatata
     8101 atacataaaa aaataaatca agataagagt aaaatactta taaaaggtat agatataact
     8161 gaaaatagtg ttgtaaaaaa cacacattgt gaagctagtg ttatattgga gtcatattga
     8221 ttagccatta tagcagtatt tgcagcagca ggcattgaag aaattactac tggtatagct
     8281 aaaatcatct tatcatttat ccatcctttt agaataaaat acacaatcac aggaagtacc
     8341 aataatctta taaaagtgac tatatataat tttttgttga caaaacaatc gagagctgaa
     8401 gaattagcaa gtaagcttcc aatgataatc atggatattg gtgtagttat acttcccaac
     8461 atctcaagtg gctcgtttat aaattggggt aacctaagtc ctgttacaaa taaaaaaagt
     8521 ccaataacaa tagctattgt agcagggctt ataagtgatt ttattgaaaa acctctactt
     8581 gtactgcctt tagataaaag ataaattcct aaagtgaaag aaaataagtt aaaaggcaaa
     8641 ttgaatattg cagtatagaa tattgcttca tgacctaaaa ctgcttcaat tacaggataa
     8701 cccataaaag caacatttga aaaaacaact atatactgat atattcccaa atctttttta
     8761 tcattacatt ttaaaatgta tttcaataaa aatgcaatta taattgaaac tatatacata
     8821 atcaaagatg taaataataa caatagtatt ttttgtatca ttttactatc aaagttaatt
     8881 tgcatggagc taattatcat tgatggtaaa aaaactgtca ttgtcaaagt tgaaagctta
     8941 gatgtgcagt ggtcatctaa taagttgaat tttctgacaa aatatccaac taaaataatt
     9001 ataaataaaa ctgcaacttg gataaaaata ttgctgaaat tcataaaatg tcccccccta
     9061 tatatacatt atataccaat tacataataa aatctaatga ttataaaatt taaaatttaa
     9121 tgttacaata tagtgacaaa aggtaaaaat tcaatattat agcattaatt atagattata
     9181 atgtataata atgtttgaga gaatatattt tataaaatgg gtaggtagcg aataatgaaa
     9241 aatacaaaat tttctataaa aaaaataaaa aatatatcta atgacccgaa agtcgtacat
     9301 actaagggtg ccctccttga aaagatgggt aagatggaaa gcgcaataga gttgtataag
     9361 agtgctgcat tggataatta cgtaaaatct caatatactt taggaaatat atatgaaagt
     9421 aaaaaacaat ttaaagaagc agaaaagtgg tattcaatgg catataaaag tggaagtgaa
     9481 gatgctgctt ttgatttagg taatatgtac tataagttag atggctatga atatgctata
     9541 tattggtatg aaaagatagc aaatttaggt tatttacaag cacaaaataa tttaggtgta
     9601 tgccatttta aaatgaaaga ttttgtaagg tgtgaaaaat ggttaaaaac agcagcagac
     9661 aagaatttag gtaaagcttg ttttaattta ggggtattat atactttttt ggagaaagat
     9721 gatgaagcat atgaatacta taaaaaaggt tctgtgcttt tagatgatga tgcaaaatat
     9781 aatttagcaa tattaaatgg aaaaaagaaa gataataaat caacagtaag tttatataaa
     9841 cagttgtaca agtcaggtca tatgaaaggt tgctttaact tgggtatgat tatggaaata
     9901 aatgataatt tagaagatgc agagaagtat tataaaaaga gtgctgatag agatgatgtg
     9961 aagtctgagt ataggcttgc atatgtatat gatagaaaag aagagctggg agatgcaatt
    10021 tattattatg agaaagcaat agaaaaaaat catacacttt ctaagtatag acttgctaat
    10081 ttatataata gagaaaatga gatagagaag gcaaaaactt attataaaat ggctgctgaa
    10141 gatgatataa cagaagcaaa aaataatctg gcaggtatat attttgaaga aaaagattat
    10201 gaaaatgcta ttaaatatta tgaggatgct atagctgttg gatgcaaaag ttcccttgaa
    10261 aatcttgggg atttatatta tcagaatcaa gatatagaaa aagcgatttc gtattattcg
    10321 aggattccaa ataatgcatc ttgtcaaatc aaattaggaa atatctatga agatttaaat
    10381 aatattgaag aagccataag ttggtataag aaagcatctg aaaatggaga tacgaggtct
    10441 agttatagat tggggtgtat atatgagagc ttaggaaata caaaaaatgc tagaaagtat
    10501 tttgaaatgg caagtagcaa aaatcatatg aatgctcgta tacatctagg tagaatttat
    10561 tttagagaag gtaaattgga agagtctaaa atgatgtttg atacacctgc caatgaaaat
    10621 aatgtgtacg ctcaacatat ggtaggatta atttatgata tgttctataa agattatgta
    10681 aattctaaat tttggtatga aaaggctaga gcacaaggtt gtgttgaatc aatatataat
    10741 ttaggccaaa tttatttaaa attaaatgat gacgcagaag ctgagaaata ttataaagaa
    10801 ggtattaaat atggaagtaa gaagtgtgag tatatgcttg caggtcttta ttataaaaag
    10861 agcctagata tgtacatttc tttagcaaat gaagagtacg ataattgtga agagatagta
    10921 gaaggctttc ccaatttaaa tatagacttt gatgaagtat taattcctcc atttaagttg
    10981 caagaagagg taatagatga agaagagtat gtaccaattt atatactaaa tataaaagaa
    11041 agtttagatt caatgctaga agggcttgaa actgaaatga ttatagatga agaacatgat
    11101 agttaaaaag tattataaga ttatatagat ttaaatatat aaaagccatt aggataaatg
    11161 taaatcaatt aattctaatg gcttttttat taatatttta taatagctta tttcttgaca
    11221 tgaaaaatgt aatcactata catattatag aggtaataag aagtattatc caaaatccgt
    11281 ttgggttatt agaaaatggc atgttgatga cattcatacc aaagaatcct gatattattg
    11341 taggtattga gaaaattagt gtaactactg ttaaaatttg cataacatta ttctgattat
    11401 tacttataat tgcactgaaa gcatccataa ttctactcaa tatatctcca tatattttag
    11461 ccatatcaag agcctgtcta ttttcaacta taacatcttc taataggtct tcatcatctg
    11521 gatacttctt tataccagca gttctaacca ttttgtttaa aacaagttca ttagatttta
    11581 gtgaggtaga gaagtatacc aaagattttt ctaattccaa taattgaacc aattctttgt
    11641 ttttcattga tttgtaaatt tctcgctcta ttatgattgt cattttattt atttttctta
    11701 agtaatgcaa gtaataagct gcatttttgt gaagaatttg aagcaaaaag cgagtctttt
    11761 taaacgtaaa aaaatcttta atatgaccaa ctataaagtc atttaaaatc ttagtctgtg
    11821 catcacaaac tgttacaata gcttcttcag ttagtataat acctaagggt attgtagtat
    11881 aatgagaaga attagagtca ctttcgtcta ctggtatatc tataagtata agagtatgat
    11941 tgtcttcaac atctattctt gagcgttctt cttcatctag tgcagattct atgctttcta
    12001 catctatatt taataaatta gaaatttcgt tgatttcact atgattaggt tcaacaagat
    12061 ttatccaaca gccatcttca aaaaaactaa gtttctctaa ttttgaatct atagttttgt
    12121 aatatcttat catggcaaca catcctttat tcagttttat ataaatctca tcatttaata
    12181 gtatctatta attaactaat ttatataact gaatatatgt attagttttg attttagata
    12241 ttattaagtg atgaaaatgt aaaaaattct ctccgcaacg gactagtatc cattgcccat
    12301 cactctcctt atttattatt ttatatatag ctatttatga aatgcgtatt ttaagcactc
    12361 tttaatataa taccttttcc aggtatataa atcaagtttt gatttgatta aataatattt
    12421 t
//
LOCUS       DAEPOZ010000116        12174 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.116, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000116 DAEPOZ010000000
VERSION     DAEPOZ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12174
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.116"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            144..1055
                     /locus_tag="KR440_002298"
     CDS             144..1055
                     /locus_tag="KR440_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HBH0699341.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            1315..1749
                     /locus_tag="KR440_002299"
     CDS             1315..1749
                     /locus_tag="KR440_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699342.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            1772..3307
                     /locus_tag="KR440_002300"
     CDS             1772..3307
                     /locus_tag="KR440_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699343.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGVIYK
                     IDCDVILVNLFELIINNCIFSTILGNEVINLEISKYEYEFLEKQFKNMYNNITEDKVI
                     GSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKNEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKTEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            3391..4758
                     /locus_tag="KR440_002301"
     CDS             3391..4758
                     /locus_tag="KR440_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0699344.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKARDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMNTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            4793..5017
                     /locus_tag="KR440_002302"
     CDS             4793..5017
                     /locus_tag="KR440_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HBH0699345.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
     gene            complement(5467..5865)
                     /locus_tag="KR440_002303"
     CDS             complement(5467..5865)
                     /locus_tag="KR440_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HBH0699346.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMVG"
     gene            complement(5868..6269)
                     /locus_tag="KR440_002304"
     CDS             complement(5868..6269)
                     /locus_tag="KR440_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBH0699347.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLVAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            6628..7614
                     /locus_tag="KR440_002305"
     CDS             6628..7614
                     /locus_tag="KR440_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0699348.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLNEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            7598..8509
                     /locus_tag="KR440_002306"
     CDS             7598..8509
                     /locus_tag="KR440_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0699349.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIRNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            8622..10190
                     /locus_tag="KR440_002307"
     CDS             8622..10190
                     /locus_tag="KR440_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0699350.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHVLEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            10265..11290
                     /locus_tag="KR440_002308"
     CDS             10265..11290
                     /locus_tag="KR440_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0699351.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDEYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            11283..>12174
                     /locus_tag="KR440_002309"
     CDS             11283..>12174
                     /locus_tag="KR440_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0699352.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIEVSDGHFV"
BASE COUNT         4824 a         1278 c         2175 g         3897 t
ORIGIN      
        1 aaatccatta taaaggctat tataaatttt aatatgctaa aaatataaaa agaatagttg
       61 acagtataga aaatatggaa taaactaaaa gtgtaatagt aatacgaaat tatatattac
      121 attgaaaggt gtgaatgatt attatgaata aaaaaataga attaataggg aattttgcaa
      181 gtgattggat taggtatagt gattatgaat ataaaattac tgaagaagat gaattatata
      241 tagttccaac agaagatgct gtgtttacca tgtataaccc atttgatgta gcagatgata
      301 taatagtaga tataattcgc ataggacaag aggcattaca agatatatca aaagagacaa
      361 aagaaatgaa actaaagaaa gaagtacttg aatttgtaaa aaagtatgga ttattgggtt
      421 taatatattc aagtgtatat aatagagata tagttggaga agaaagagta ctgatgattg
      481 aaaagaatta tattacaaaa gagaatatat tgagtacaga tgaatatgta aacatgttta
      541 taccatttgc aagtgatgaa gatgtgtatt ttaaaaaata tagaaggggt atagatgtat
      601 caaagagaga agaatctcca aagttttttg gaaagagacc attgatttta gatttagtat
      661 tttcaaagtt ctacactgaa caactagatt ggattgtaag ttttgctaaa gatatgtgta
      721 agcattttaa tcaaatttgt gtatataaaa atacatctaa tctattaact gaaagtgtta
      781 ctattatgtc tggagggttt aatcctcaaa agataggatt ttctataagc caattagata
      841 aaccaattat tgcatgggag tttgattctt tgaaaacagc tattgagact atctatgcct
      901 ttgcaattac aagtgaacct tcttctttaa ataaatgcaa gtactgtggt aaaatttata
      961 ttcctacaaa ttctaaatct caatactgtt caccttcttg tagaaattgc tcaaatgtaa
     1021 agaaaagtag aagtagagcc aaaaaagata aataaaatta ataaatatat cattgtttag
     1081 attttagtaa ataactactg aattaaaagt tgttatagaa aattatatat ctaaatgatg
     1141 gtattttttt gttgagaaaa ttgaaaagtt taattgagat agtaattttg agttgacaaa
     1201 aaccactttt aacactacta ttataagtgg ttggtaaaat aatatacact aatttaaata
     1261 tagcataaaa aacaaaaaaa gtcaatgaaa aaaataaaaa aggagaattt aaaaatgata
     1321 tcacctatta ttttgagttc tataaatcag aatttaaagg aaatagaaag aaatgaactg
     1381 cttgaaacta atatagaatc tggagattat ggtctagctc tttctgaatc tgatgtaaaa
     1441 gatataataa attcgagaga taatacttta aaggggtatg gaagaataga attagatata
     1501 aaagttacaa aacagttaat agaaaatata tatacatcac aatatacaaa tgtggacaat
     1561 tatttagagg ctattaatga tatgcaagaa attttttatt atttaaaaaa tgaaactgat
     1621 gacaagatat gtgatgatga gattatagaa atactgggtg aattttatga aaaattttca
     1681 ggcaatatgg acaatgtaag aggagaagca gacgaatttg caaagaaatt taaatttgga
     1741 gaagtttaat atatgatagg agagattttg tatgttaaat aatttaagta atattaatat
     1801 aaacgaaaat aatttaataa agaaccaata ttccatatct ctacttaagg aatgtctaaa
     1861 ttgtaaagtg atagatgaaa gagaagtata taatatacaa caagaaatat ctctaatact
     1921 tatggatttg attaaaaaat atacaaatgg tcaaagcaca tcagtaaaaa cagaagttgc
     1981 cgaaaaactg ttgatttcaa tatggtatgc tattgatgca tatataaata agcttggaga
     2041 tatagaaaaa agagttgtgg agttgactgc taagaatgtt aaaaaagtgt accttggagg
     2101 cattgaaata ttagaggaag agttagttag gctgaaagat ttttataaac taatgatgag
     2161 tagaaaattg gatactaatt caatagcata taatgatacc ttgatggagt tatcaagttt
     2221 ctttaaaagt tatattgtaa aatttgaagc tcaggacgtt ccagtaagta tagattatcc
     2281 attggcacta gatgatatgg atgttaaggg tatttattat gtgaagaatt atattgagag
     2341 tatagatata gaaaatagat tttgtaacct atttaagaaa aaagatagag aaaaattatt
     2401 ttatgactat ggggtgattt ataaaataga ttgtgatgtt atacttgtaa atttatttga
     2461 acttattata aataattgta ttttttctac tattttagga aatgaagtga ttaatttaga
     2521 aatttcaaaa tatgagtatg aatttttaga aaaacaattt aaaaatatgt acaataatat
     2581 tactgaagat aaagttatag gctctgaaga aagtaatata attgaattga ttgaagtaga
     2641 tgatagtttt aacactgaag tagatagtaa tttggattat aatgattttg aagaaattaa
     2701 cagagaaatt agaataaata aaaaaataac tttactactt atggaagcaa ttgaagtatt
     2761 aataaaaaga ttaagcatag aagatgaaga tgtaataaga tatattaaaa aatatgaaaa
     2821 gatatttatt gaatctgtgc taagctcaat aaaaagtaac acttttaaaa gtatggtaac
     2881 tgtgagtaaa aataaaaatg aacacttaga agactatatt gttgatgatg aaagtaaatt
     2941 ggatgatgaa tcgtttagag agatattcaa tgagatatta gatagtacat ctataattga
     3001 aaaaataaga ataataaagg aaaatatcaa tgctaaaaaa gactttatcg atattcttga
     3061 atctgaatgt ttatttggag aagagtattt aatgttgttt gcatcattaa gtgaattaga
     3121 attggcaata ttgggtagtg tagtatttta tgaagatatt agaatgagag aaattaatat
     3181 attagaattt atgttaaatg agaaaactga aactacttta tggaagacag agtacattaa
     3241 atttatgaag aggcaaagtg aagataagat atactccata gaagagcata tgaataaaat
     3301 taattaacaa aaaattgtga ataaatagaa tgttagtata taatataatc tgtaaagcat
     3361 atcaaaaata catatatttg gaggaaaatt atgaaagagt atatcataaa taatgttgaa
     3421 gaaataagag aagaattaat tgatttatct aagaaaatat gggaaaatcc agaattagcc
     3481 tttgaagaaa agtatgcaag tagcattcaa aaagaatacc taaagtcaaa aggatttaaa
     3541 attgaagaag tagaaaattt acctactgga tttatagcaa gttttggaga gggtaaacct
     3601 gtgattggca tacttggaga atatgatgct ttaccagagc tatcacaatg tgtgagtgca
     3661 gaaagaaagc cattggtaga aggaaaagct ggacatggat gtgggcataa cctgttgggt
     3721 gtagcaggtg taggagcagt tgtttctata aaaaaattaa tagaagaaaa taaatttaat
     3781 ggaacaataa aatattttgg ttgtcctgct gaagaagaag gtggaggaaa gacagttatg
     3841 tgtataaatg gctgttttga tgatgtagat tgtgcattta catggcatcc ttttgatata
     3901 aatgcacctt ggagaggtgg aagtctggca aacttatctg taaaatttaa atttaaaggg
     3961 ataacagccc atgcagctca agctcctcat aatggtagaa gcgcacttga tgctgttgaa
     4021 attatgaatg ttggagctaa ttatttgaga gaacatgtta ttgactctat aagaatgcac
     4081 tatgttataa ctaatggtgg tggaagacct aatgtagttc ctggatttgc tgaaagttgg
     4141 tactttatta gaggtaaaaa agcaagggat gcagaacatg ttcttgatag attaataaaa
     4201 gtagctcaag gagcagcaat gatgacagaa actgaaatgg agtataaggt tacagatgga
     4261 atatatgact atataccaaa ccaatgtttg actgatttag tttatgacaa tatggttttt
     4321 gttggttgcc ctaaaaatac tcctgaagaa gaagaatttg caaaaaaatt gtgtgatact
     4381 cttactagag aagaaagatt aggtgtagca acatcattac aaaatgataa atctattgta
     4441 gaaagttata tacacaagga aatagttgac ggtgacaaag ataaaggact agctggttct
     4501 actgatgttg gagatgtaag ttatgtaata ccagtggctc aatttgcaat ggcagcttgg
     4561 cctgttggaa ttgcaagtca tacatggcag tcatgttcat cagcaggttc aaatatagga
     4621 ttttctgcta tgataaattc agctaaagta ctggcttgta gtgcttatga tgtatttatg
     4681 aatactaaaa ttattgatga agcaaaaatt gaatttgata aatcattaga tggacaaaaa
     4741 tttaaaccat tggtataatt ttggtaataa aatatttctg gggggaatag ctatgagaga
     4801 aaaagttgaa aaagtgcttg aggaaaagat aaaaccagta ttacaaagag atggtggaga
     4861 tgtagagctt atagatgtaa atgaaaatgg tgtggtttta gttagactac aaggggcatg
     4921 tagtgggtgt ccaggagcga ctatgacaat caaagctatt attgaaaatg ttttggtgag
     4981 tgaagttcca ggagtaactc aagtattagg tgtttaatag ttagcgttta ttaaatgtat
     5041 gttatacata agtatttaat aaagaagtaa aaaaaatagt gaagcaatat atatgaaaaa
     5101 gtatgataaa gataataaaa cattatagaa ataatgaaat atttcatatg tatatgaaga
     5161 aataagaaaa aatagtgaag caatatatat atgaaaaagt atgataaaga taataaaaca
     5221 tttatagaaa taatggaata tttcatatat acatatgaaa aaatagtaaa acaatatata
     5281 tatgaaaaag tgtgagaaaa taataaaata ctatagaaac aattaaatat tctatatagg
     5341 tattaagaaa catgaaagaa ataatggagt attatagata aatatgtaaa agtgtgaaag
     5401 aaggatgtag ataaatttgt cttatacaaa tttacctaca gtatttctta cacacttttt
     5461 attttactat ccaaccatac caataaacat acctataaag tatggaacta accatatagt
     5521 ccatacaact atactaaact tatgaaagaa ttttttcttt tcctcatcat ctttgtatag
     5581 gacaaaagta gcccaacttg catgaaaaag cataagcaca attgcaagaa gacctgtaat
     5641 actatgaagg ttttgtgata aagcagattt tacttcaaaa gtatggctgt tggctatgtt
     5701 actcattgta aatgttccta aagtatcaaa tataaaccca agccaaaata taattacatg
     5761 tttctttttt aaaatttttg cctttctttc tccaaataca cctatagtgt agaaaattaa
     5821 agctgatgtt ataaatacta ttgcaagtat taatttataa ttcataatta tctctccttt
     5881 acttttgcca atatatttct aagatttttt actaagtcac tcaattcgtt ttcatcacaa
     5941 tttttaaaaa tatcatcaaa actttcttgc attttaattt taaaatttgt ggcaaattca
     6001 aatcctgttg ttgtaaactt aacgtatgta tttcttttat cattagattt cttgaatttt
     6061 tctatatatc caatctgttc taatctgctg attatgcctg aaaccgtacc tttagctaaa
     6121 gacatttcat cacatagttg tgatattgta agttcttgat tatgagctac cagttttata
     6181 actataatct gttggtgtgt taatccattt tctttaaaat tttcttctac tgtgtacata
     6241 gttttagaat aaagttcttt taagagcatg gctattctaa aacagtcata attacttttc
     6301 aataaaatca cctcttaaat ggtttgcata caaactaata taatatactt ttcttatata
     6361 gtcaacctaa aattttataa ttttatagaa aataatgaag aatagaacat aaaaatttta
     6421 ttttaggtat aaataaaata atttgatgaa attttaacaa tttttaaaaa gtttgtttac
     6481 acagttaata aatgatgcta aaattaactc atagataaat atagaaaatt taattttgtt
     6541 taatgataat ggaaccacga aggttttaac gttactttac gtttgctttt atgtggtttt
     6601 tttattgcaa caaagggggg ttggggtttg ttaaaggata tactgaaaaa gttgttgcag
     6661 tttattttag taatgttttt attatctttt attgtttttt acatggcaag acttgctcca
     6721 ggagacccat taatctctta ctatggggat ggagtagaaa gaatgagtac acaagaaaaa
     6781 gaaaatgcta tgaaaaaact tggtttgaat gaaccaatat acagtcaata tataaaatgg
     6841 atttcaaatg catcaaaagg ggaatttgga atttcattta aatataaaca aaatgttact
     6901 tctgttataa atgatgtcta tataaacact ataatattag gtggttcagg gtatatactt
     6961 acctttgtgc tagccttatt gttaggaata ttttgtacat tacatgaaga taagctcatt
     7021 gatagaatca tatgcaaatt aggaacaata accaactgta ttccatcatt ttgggttgcc
     7081 ttagtactca tacttatatt tagtataaac ttaagtatac ttccaagtag tggtgcttat
     7141 tcaatgggag aagagtccag tatattaagt aggatatcac atctaatctt acctcttaca
     7201 gtacttatat taagccatct atggtattac acatatatga taagaaataa attactagag
     7261 gaaataagag aagattatgt gcttttatgt aaagcaaaag gtttaaataa tagaactatt
     7321 gtttttaagc attgcctgag aaatataatg ccatcatata ttagtataat ggccatttcg
     7381 atacctcata tactaggagg aacttatgta gttgaaaaag ttttttctta tcctgggtta
     7441 ggaacacttt gttttgaaag tgcaaagtat catgattata atatgttatt agttttatgc
     7501 ttaataacag gggctttagt agtatttggg aatatgttag ctcaaattat aaacaataaa
     7561 atagacccta gaatgaaata tgatagaggt gatactaatg aagccacaat atagtgattt
     7621 tgaaattgta ggagaaaatt atattttagt agcagaagat gaacatatag aaacaaagaa
     7681 aaccttatct aaaaaaatta aagaattacc atatatatca attattattt tatcaatcat
     7741 agtcattgga agtgtattct catcttttat aatgacacat gaaccaacat acatggattt
     7801 agcaagttct aatttagctc caaataaaac ttttttattt ggaacagact caatgggtag
     7861 agatatatat tctatgatat ggtatggagg aaaaatatct ttatttatag gactattttc
     7921 cactgtaata tcaactgtta taggaattgt ttatggtagt ataagtggtt cagcatctga
     7981 actcgtagat gatgcaatga tgaggtttac agaaatcata cttagtatac cttcaatatt
     8041 aattataata tttgtacaag ctatacttgg aaattcaaat ccagtatcta tgtccatagt
     8101 aatagggata acaagttgga tgaatatatc taagatagtg agaacagaag ttagacaaat
     8161 aagaaatagc gaatacatac tagcagctaa gagcatggga ggaggattct tctatgtatt
     8221 aaagcaacat ttacttccaa attttgtggc atcaataatg ttcatggttg taacaaacat
     8281 aggcgcagct attggtactg aatcgacact tagcttttta ggaattggtt taccaataga
     8341 aattgtttct tggggaagta tgttatcttt gtcagaggaa gcattacttt caaatagatg
     8401 gtggattata ctgattccgg gtatattttt ggttaccaca ttggtgtgta taacaaatat
     8461 aggtaattat ataagaaaga ataataataa aaaatctagt aatttataaa tcagcatttg
     8521 agtactaaat aagcaaatac tgaggtattt gaaatatcta tttaaataaa tggggtcaaa
     8581 gctttgaaaa atattttata aaaagcaaaa gggggagcat catgaaatta aagaagttaa
     8641 aagttttaag tttagtaatg atacttagtc taatggcagg ttgttctagt ggaggagaca
     8701 aagataagaa ggcagatacg cctaaagatg gaaaggtact tgtttatgga agtaatgact
     8761 atacaagtat aaatcctgca ctatatgaac atggagaaat aaattcatta atatttaatg
     8821 gattaacagc tcatgatgaa aataataagg tggttccttg tctagcaaag gactggaaat
     8881 ttgatgaggc aacaaataca tatactttta atttaagaga tgatgttaag tggcatgatg
     8941 gagagaaatt tacggcaaat gatgttaagt ttacaataga aactataatg aatccagata
     9001 atgcttctga gatagcatca aattatgaag atataacaaa aattgatgtg gttaatgata
     9061 atactataaa aattacttta aaagctccaa atacagcaat gcttgattat ttaacagtag
     9121 gagtattacc aaaacatgtt ttggaaggta aggatatagc tactgatgaa tttaatcaaa
     9181 aaccaatagg tactggacca tttaaacttg aaaaatggga caaaggtcaa agtataacac
     9241 ttgttaagaa tagtgattat tttgtaaaag aaccaggatt agataaagta gtctttaaga
     9301 ttgttccaga tgataaggct aaggcaatgc aattaaaatc aggagaatta gacttagctc
     9361 aaataactcc taaagatatg tctaactttg aaaaagacga aaagaatttt aaagtaaata
     9421 taatgaaaac agccgactat agaggtatat tatacaattt taattctaaa ttctttaaag
     9481 ataaaaaagc taaaggatta ccaaatgctt taagctatgc aatagataga aaagcaatag
     9541 ttgacagtgt attattagga catggtgtac cagcatattc acctttacaa atgggaccat
     9601 ataataatcc tgatatagaa aagtttgaat ataatccaga aaaagctaaa caagaaattg
     9661 aaaagctagg ttggaaatta ggttctgatg gaatctatga aaaagaaggt actaagttag
     9721 ctttcgagat aacagcaggt gagagtgacc aagttagagt tgatatggct aaaatatgtg
     9781 cacaacagtt aaaagaaatt ggtgttgatg ctaaagctgt tgttgtaact gaaacagatt
     9841 gggctaatca agatgctcat ttaataggtt ggggaagtcc atttgaccca gatgaccata
     9901 catataaagt atttggaaca gataaaggtg caaattacag tgcttactct aatcctacta
     9961 tagataaaat acttcaaaaa gcaagagaaa cagaagataa agatgaaaaa ttaaagttat
    10021 ataaacaatt ccaagtagaa atgactaagg atatgcctta tacatttatt gcttatatag
    10081 atgcaatata tgttggaaaa ccaaacataa aaggattaac acctgataca gttttaggtc
    10141 atcatggtgt aggtatattc tggaacatag ctgactggac aattgaataa tactgaaatt
    10201 aattagaaac cttagggata aaaatatatc cctaaggttt gaatagcata ggggttgatg
    10261 gtttttggaa catttattgg aggttaataa tctatctgta agttttaaag tagaagaagg
    10321 agaagttcag gctgtaagaa atgtatcttt taatctaaaa aaaggtgaga cattggctat
    10381 agttggagaa tctggttgtg ggaaatctgt actgtgtaag agtttgatga ggatactccc
    10441 atacaatgga tatattaaaa atggagaggt tttacttaaa tcaagtgatt tggttaagaa
    10501 gtctgaaaaa gagatggaag atataagagg aaagaatata tcaatgatat ttcaagaccc
    10561 tatgacatct ctcaatccaa ctatatcaat aggaaagcaa attgcagaag ctgtaatcat
    10621 tcatcaaggt ataagtaaaa gtgaagcaaa aaagagagcc atagagctta ttgaattggt
    10681 tggaattgat aatcctgaga aaagatttaa acaatttcca catcattttt caggtggaat
    10741 gagacaacgt atagtaatag ctatagcact tgcctgtaat ccagatgtat tgattgctga
    10801 tgaacctaca actgctttag atgtaactat acaagctcaa ataattgatt taataaagga
    10861 tttacaacat aaaataggtt tgtcaataat atttataact catgatttag gagtagttgc
    10921 aactatagct gatagaatag cagttatgta tgcaggaaag attgtagaaa taggaactgt
    10981 agaggatata ttttatgacc ctagacatcc atatacttgg gggttgttgg gttcattacc
    11041 tactttagat tcacaggatg aatatttata taatatacct ggaatgcctc caaatttatt
    11101 gaacccacca aaaggagatg cttttgcaat aagaaataaa aatgcactta agatagatta
    11161 tgaaaaagag ccaccaatgt ttaaaattaa tgatacacat agtgcagcaa cttggttatt
    11221 gcacccagat gcacctgaag tagatgtacc agttagagta aattgtggaa gggtgatttc
    11281 taatgaatag agaaaaaata ctggaaataa aaaacttaaa gcaatatttt catttggaca
    11341 aaagtactac tgtaaaagca gttgatgata taagttttga tatatataag ggtgagatat
    11401 ttggattggt aggagagtcg ggttcaggaa aatctacaac tggtaaaact ataataagac
    11461 tacatgaatc tactggtgga gaagtaattt acaaaggaaa ttgcatatca gataaaaaaa
    11521 cgtacaaatt tataaaaaaa gatgtaaaca aaagtatgca gattattttt caagattcta
    11581 cttcatcatt aaatccacgt atgacaattg cagatataat ttcagagcca ctaaaaattc
    11641 aaggtatctg taaaaataaa acagatagaa tgaataaagt atatgagatg ctaaagctag
    11701 taggacttga tagaagttat gcaaataagt atccatctga tttttcaggt ggacaaagac
    11761 agcgtattgg catagcacga gctctttcag tagacccaga atttataata gctgatgagc
    11821 ctatagcttc tttggatgtg tctatacaag cacaaatagt aaatttattt aaaaaattgc
    11881 aacaagaaaa gaatttaact tgtttattta tagctcatga tttatctatg gtgagacata
    11941 ttagtgaccg tataggagtt atgtataatg gaaaattagt agaattggca gattcaaatg
    12001 aattgtataa taatcctatt catccatata caaaatcact actttctgca attcctgttc
    12061 ctgaccctag atatgctaag tcaagaaata gaatagagta taattctaat ggatatgatt
    12121 gttcaaatga aaagtcatta agttggattg aggtttcaga tggacatttt gtat
//
LOCUS       DAEPOZ010000117        11698 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.117, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000117 DAEPOZ010000000
VERSION     DAEPOZ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11698
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.117"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            65..1363
                     /locus_tag="KR440_002310"
     CDS             65..1363
                     /locus_tag="KR440_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HBH0699353.1"
                     /translation="MNLTQKRWIILIASCFINLCIGSIYAWSVFASPMAEHLNSLAGT
                     TFTAASLAIVFTVTNSVGPITMITGGMINDKFGPKKVVFVGGLIFGIGMILAGFSKGL
                     PMLILSYGIITGLGVGMVYGCTISNSIKFFPDKSGLVGGITTAAYGFSSVIMPPVANW
                     IINKYGVTSAFKSIGLVFLIIVCVSSFFIKKCPADFTPEGWVPPEKKGKVRVVINKNW
                     KEMLSTPIFYLMIIILTCGAFYGLMCVSLASSLATNMIGMTSSDSAIVVSILALFNTL
                     GRIVAGYISDKIGRINTLSGALVISIFGLLCLLFSREGDVATFYIGIIIIGICFGSFM
                     GIFPGFTVDKFGPKNNSVNYGIMFIGFAFAGYIGPAVMKNVYSVDGNYQRAFIIAIII
                     ALIGFILTFLYKYINKIQNLKIENLIKGDIKNGKKISKSM"
     gene            1335..3284
                     /locus_tag="KR440_002311"
     CDS             1335..3284
                     /locus_tag="KR440_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBH0699354.1"
                     /translation="MERKYPNLCKPIKIGNVTFKNRMFSAPMGGTDITADCTIGSKST
                     AFYELRAKGGAGAVTISECMVHPETDGSHAYHLDLKIVDSLASFTYTADAIRRHGAIP
                     SVELSHSGQYSGTYLVDKDKKRGLSQWGVSPSVRPDGLEIKELTEEIIAEIVESYGNV
                     AALAKRAGFEMIMIHGGHGWLINQFLSPYFNKRTDKYGGSLENRVRFAKEVLDSVRKA
                     VGPGFPIEFRMSGSELFEGGYDLDYGTEIAKLLESRVDLLHVSAGTYQRGFAVTHPSM
                     FLEHGCNVYLAEEIKKHVNIPVATIGALNDPEMMEEIIASGKADVIYMARALLADHEL
                     PRKVMSNQDEKIVKCLRCFTCMAERATTSTRRCTVNPLIGRELDGTEVVSVLKPKKVL
                     IAGGGPGGLQAAITAVKRGHKVILCEKTNELGGILKGEQALPFKYKMYELGNTFGKIA
                     KDLGVEVRLNTTVTKEYVENENVDALIIAVGSEPLVPPIEGLDGENVVIVNDYYVEKE
                     KVTDEVIVLGGGLAGCEAAIHLAQEEKTVHLVEMRAELAPDANIRHRPILLEEIERQG
                     IYVYTEHKGLSVTTEGVVCMNKSGNKINIPGTSVICALGQKPRREVVDTLLDSAPYVA
                     QIGDCVRASTITNAVYQGHHAALDI"
     gene            3721..3858
                     /locus_tag="KR440_002312"
     CDS             3721..3858
                     /locus_tag="KR440_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0699355.1"
                     /translation="MINENLKSLRKIHQYTQEELAEKLNVSRQSIAKWESGVSQTKGY
                     Y"
     gene            4086..4460
                     /locus_tag="KR440_002313"
     CDS             4086..4460
                     /locus_tag="KR440_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HBH0699356.1"
                     /translation="MFNLIEMLFPIMFILIFIIIVFTFVKGIATWFKNNNSPRLTVSA
                     RIVAKRQNTTHNNQPNAGDISGAHGFHTISSTSYYVTFQVDSGDRIELSVSGSEYGVL
                     AEGDKGQLTFQGTRYLTFDREN"
     gene            4476..5123
                     /locus_tag="KR440_002314"
     CDS             4476..5123
                     /locus_tag="KR440_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699357.1"
                     /translation="MNKFLSQIRRIDNEISITRKIINTIFILCFGIVLGTFAKFLDTT
                     ASNTLPFIFEYLDISNFFGRFAIWLLIALYIAIYSHSSIRASLNVLVFFIGMVSSYYL
                     YSYFVAGFFPKNYAMIWLGFTVISPLLAFICWYAKGKSKISFILSVIIIAILFNFTFI
                     YGWIYFDVYSILEVIVFGCALIALKRNTFRETTYMILSAVVIAVILNMLVPFHFG"
     gene            5503..6582
                     /locus_tag="KR440_002315"
     CDS             5503..6582
                     /locus_tag="KR440_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBH0699358.1"
                     /translation="MNDMKFIETLKQKRNVCDYSQSRLAQELQINRQNLNEIENGKTK
                     ASKEMKHILLHYLDYCNCTQPFTLTIDYLRVRFPTTDALEIIKNVLAMKSEYFIHEDY
                     GMYAYEEQYIYGDISVNASKDSSMGVLLELRGMGCRDLEYVLQVRGIDWYCFLRCCID
                     YQGVFKRIDLAVNDMGGLLDIEILRERYYANKVWKRSRTHEAVDSGKLSGTHGDTAKT
                     FYIGSKNSSIYFCLYEKEKEQKSKGIKTDIKNRFEIRLKNGKAGQTVEQLVFSRNPEQ
                     TIASFILTQIDFPDYILWDIFLDNVTTSLPFIMTPVAVNMDKIKRWLERQVMPSLLMI
                     KEIEKKTGANYLEEIDRHTRLTEKQ"
     gene            7404..7617
                     /locus_tag="KR440_002316"
                     /pseudo
     CDS             7404..7617
                     /locus_tag="KR440_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
     gene            7729..9342
                     /locus_tag="KR440_002317"
     CDS             7729..9342
                     /locus_tag="KR440_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH0699359.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFERPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIVEKIFSLAYQGYGSAKITKVLREEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     ATNTANKTPYSYYACSYYGQFGKGTCSMHYIRYDVLYQAVLKRLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASAFKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITNLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEAITNEDNERIQEIEIYYRFIGKVD"
     gene            9371..9694
                     /locus_tag="KR440_002318"
                     /pseudo
     CDS             9371..9694
                     /locus_tag="KR440_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
     gene            9726..10913
                     /locus_tag="KR440_002319"
     CDS             9726..10913
                     /locus_tag="KR440_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBH0699360.1"
                     /translation="MSKIVFFSILAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIK
                     PKERKRKLIYISLGTINNKNVSFYKNCINAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFITHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            11105..11509
                     /locus_tag="KR440_002320"
     CDS             11105..11509
                     /locus_tag="KR440_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBH0699361.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYHALYVNKVMLQI
                     YDVENNNKLLRSNEELKSVKYPPIYLKYKPDK"
BASE COUNT         4269 a         1493 c         2315 g         3621 t
ORIGIN      
        1 agaaaaaatt gtgataatct taactaagaa attctattta ataaatttat cgaggaggag
       61 agtaatgaat ttaacacaaa aaagatggat tatattaatt gcaagttgtt tcatcaattt
      121 atgtattggt tcaatatatg cgtggagtgt atttgcatca cctatggcag aacatttaaa
      181 tagtcttgct ggaacaactt ttacagctgc tagtttagca attgtattta cagttacaaa
      241 ctcagttgga cctattacta tgattacagg gggaatgata aatgataaat ttggtcctaa
      301 aaaagttgta tttgtaggag gattaatttt tggtataggt atgatattgg caggtttctc
      361 aaaaggattg cctatgttga ttttatcata tgggataata acaggtcttg gggttggcat
      421 ggtttatgga tgtacaataa gtaattcaat taaatttttt ccagataaaa gtggattagt
      481 tggtggtata acaacagcag cttatgggtt tagttcagta ataatgcctc cagttgctaa
      541 ttggataatt aataaatatg gagtaacttc tgcatttaaa tcaataggtt tagtatttct
      601 tataattgta tgtgtttctt ctttctttat caaaaaatgt ccagcagatt tcacaccaga
      661 aggatgggta ccaccagaga aaaaaggaaa agtaagagtt gttattaata aaaattggaa
      721 agaaatgctt tcaacaccta tattttatct aatgattata atattaactt gtggagcttt
      781 ctatggatta atgtgtgtgt cattagcatc ttctctagca acaaatatga ttgggatgac
      841 aagttcagat tcagcaatag tggtttcaat tttagccttg tttaatacat taggccgtat
      901 tgttgcagga tatatatcag ataaaattgg tagaataaat actttatcag gtgcattagt
      961 tatttcaatt tttggtttat tatgtttgtt attctcaaga gaaggagatg tagcaacatt
     1021 ctatattgga ataataatta taggtatatg ttttggttca tttatgggaa ttttcccagg
     1081 atttacagta gataaatttg gacctaaaaa taatagtgta aattatggaa tcatgtttat
     1141 tggttttgct tttgcaggat atattggacc tgcagtgatg aaaaatgtat attctgtaga
     1201 tggcaattat caaagggctt tcattatagc tataattata gctttaatag gttttatatt
     1261 aacattttta tataaatata taaataaaat acaaaattta aagatagaaa atttaataaa
     1321 gggagatata aaaaatggaa agaaaatatc caaatctatg taagccaatt aaaattggaa
     1381 atgtaacatt caaaaacaga atgttttcag cacctatggg gggaacagac ataacagcag
     1441 attgtacaat tggctcaaaa tcaacagcct tttacgaatt aagagcaaaa ggtggagcag
     1501 gtgcagttac aattagtgaa tgtatggttc atccagaaac agatggttca catgcatatc
     1561 atctagactt aaaaatagtt gattccttag caagttttac ttatacagca gatgcaattc
     1621 gccgtcatgg agcaatacca agtgtagaac tatctcattc aggacaatat tcaggaactt
     1681 atttagttga caaagataag aagcgaggat tatcacaatg gggagtaagt cctagtgttc
     1741 gtcctgatgg attagagata aaagaactta cagaagaaat aattgctgaa atagtagagt
     1801 catatggaaa tgttgcagca ttggctaaga gagcagggtt tgagatgatt atgattcatg
     1861 gtggacatgg ctggctaatc aatcaattct tatcacctta ctttaataaa agaactgaca
     1921 aatatggtgg ttctttagaa aacagagttc gttttgctaa agaagtttta gatagtgttc
     1981 gtaaagcagt aggtccagga tttccaatag agtttaggat gagtggttct gaattatttg
     2041 agggagggta tgatttagat tatggaacag aaattgctaa attattggaa tcaagagtag
     2101 atttacttca tgtatcagca ggtacatacc aaagagggtt tgcagtaaca catccttcta
     2161 tgttcttaga acatggatgt aacgtatatc ttgcagaaga aattaaaaaa catgtaaata
     2221 ttccagttgc tactatagga gcattaaatg acccagaaat gatggaagag ataattgcta
     2281 gtggaaaagc agatgttatt tacatggctc gtgcattact agctgaccac gaattaccaa
     2341 gaaaagttat gagtaatcaa gatgaaaaaa tagtgaaatg cctaagatgc ttcacttgta
     2401 tggcagaacg tgctaccact tctacaagac gctgcactgt taatccactt attggacgtg
     2461 aattagatgg aactgaagtt gtttcagtat taaaacctaa aaaagtatta atagcaggtg
     2521 gtggaccagg aggattgcaa gcagccatta cagctgtaaa gcgtggacat aaagtaatac
     2581 tttgtgaaaa aactaatgaa ttaggtggaa ttttaaaggg agaacaagca cttccattta
     2641 agtataaaat gtatgaatta ggaaatactt ttggaaaaat agcaaaagat ttaggtgttg
     2701 aagtacgttt aaatacaaca gttacaaaag aatatgttga aaatgaaaat gtcgatgcat
     2761 taattatagc agtagggtct gaacctcttg ttccaccaat agagggtctt gatggagaaa
     2821 atgtagtaat agttaatgat tattacgtag aaaaagaaaa agtcactgac gaagtaatag
     2881 ttttaggtgg aggattagca ggatgtgagg ctgcaattca cttagctcaa gaagagaaga
     2941 ctgttcactt agtagagatg agagcagaac ttgccccaga tgcaaatata aggcatcgtc
     3001 caatattatt agaagaaata gaaagacaag gtatatatgt atatacagaa cataagggtt
     3061 taagtgtaac aacagaaggt gtagtatgta tgaataaatc tgggaataaa ataaatattc
     3121 caggaacctc agtaatatgt gcacttggac aaaaaccaag aagagaagtg gtagacactt
     3181 tacttgattc tgctccatat gttgctcaaa ttggagactg tgttcgtgca tctacaataa
     3241 caaatgcagt ataccaaggg catcatgctg cgctagatat atagttaaaa agacgtagat
     3301 agtattgttt tattataaaa aaggagtgtc tcaaaatgaa ctttttagtt catgagacac
     3361 ttttttacat gaaattaaat atatttttat catttcaaca atgaaaaaag attatcttta
     3421 ttttgagaca gcacttttgt tattatgatt ttaattctta ttgcaagttt tcatttgatt
     3481 gatgagtaaa actttttaag atgtagaagt atttaacaat gtaaatgacc ctaacttaaa
     3541 tactttagcg aaggttgaca gatttgtgtt tgcttttatc tattataact taaggaaaca
     3601 tgttacctga acaataaatt tatataataa aagtatataa atatatatat gaatgttaag
     3661 attagttgat agaatttaaa taataaatcc aatattataa acataggagg tatgaaaaat
     3721 atgattaatg agaatttaaa aagtctaaga aaaatccacc agtatacaca agaagaatta
     3781 gctgaaaaac ttaatgtttc tagacaatca atagcaaaat gggaaagtgg agtttcccaa
     3841 acaaaaggat attactgaaa taacctgtgt ttacctgctt gtagagaaaa gaatattgct
     3901 attaagccag agatactaca taaatgtgtt ggtatctctt tttcgttaat gcaacacagt
     3961 aaattatttc attttcctaa ttattttaga ctgataagta actttttaaa tgaagaaaag
     4021 aaatgacaat acttaaagga gcatggtata cttaaaggag aatttcatta aggaggaaaa
     4081 agagattgtt taatctaata gaaatgttat ttcctataat gtttatactg atttttataa
     4141 taattgtttt cacttttgtg aaaggaatag ctacttggtt taaaaacaat aattctcctc
     4201 gcttaactgt gtcagcaaga atagtagcaa aacgtcagaa taccactcat aacaatcaac
     4261 caaacgctgg tgatatatca ggagcacatg gatttcatac aatttccagc acctcgtatt
     4321 atgtgacatt tcaagtagat agtggtgata gaatagagct gtctgtatct ggctcagagt
     4381 atggagtgct tgcagaggga gataaaggac agctgacatt ccaaggcaca agatatttaa
     4441 catttgatag ggaaaattga atttagaggt gcattatgaa taagtttttg agtcagataa
     4501 ggcgtataga taatgaaata tctattacta ggaaaattat aaatacaata tttatactgt
     4561 gttttggaat agttttagga acttttgcaa aatttttaga tactacagca agcaatactt
     4621 taccatttat atttgaatat ttagatatta gtaatttttt tggtcgtttt gctatatggt
     4681 tattgattgc tttgtacatt gcaatttata gtcattcttc cataagagca tcattaaatg
     4741 tgttagtatt tttcattgga atggtttcaa gttattacct atattcgtat tttgttgctg
     4801 gattttttcc aaaaaattat gcaatgattt ggcttggttt tactgtcatt tctccattgt
     4861 tagcttttat ttgttggtat gctaagggga aaagcaaaat atcttttata ctttcagtaa
     4921 taataattgc aattttattt aattttacat ttatttatgg ttggatttat tttgatgtgt
     4981 attcaatttt agaagtgata gtctttggtt gtgcattaat agctttaaaa cgtaacacct
     5041 ttagggagac aacgtatatg atattgagtg cagttgttat tgctgttata cttaatatgt
     5101 tagttccatt tcattttggt taggcagatt ttaagattat tatagataga atataaattt
     5161 cacgaaccgc cgaagcgatc cgccagcggt ggcggtcgta agacatatcg ccacgccagc
     5221 ttgctggcga ggtggcggtt cactttggcg gtttggcgag cctgcgagct ttctaagccc
     5281 ccgttatcta acaggggggg actattacaa aagacaagag caaaaacaaa tataaaactg
     5341 ctgatatgca acgatttata aggttgtaag aacttatttc actttacacc ctattctttc
     5401 tacccgacag gtctaaaaca gcatatcaat agaaaataaa tataagatac aaggtgtatg
     5461 ctcctatgag tatatgcctt tttttgattg gaggaatgaa gcatgaatga tatgaaattt
     5521 attgaaacat taaagcaaaa gagaaatgtc tgtgattatt cgcaatcacg actggcacag
     5581 gaactacaaa tcaacaggca gaacttaaat gaaattgaaa acggaaaaac aaaagccagt
     5641 aaagaaatga aacatatact cttacattat cttgattatt gtaattgtac acagcctttc
     5701 actttaacga ttgactatct gcgtgttcgt tttcctacca cagacgcatt ggaaattata
     5761 aaaaatgtgc tggcaatgaa aagcgaatat tttatacatg aagattacgg aatgtatgct
     5821 tatgaagaac agtatatcta tggggatatt agtgtaaatg cttctaaaga tagttctatg
     5881 ggtgttttat tggaattaag aggtatgggg tgtcgggact tagaatatgt tttacaggta
     5941 agagggatag actggtattg ctttttaaga tgttgtatag attatcaagg tgtttttaaa
     6001 cgcatagatt tagcagtcaa tgatatgggc ggattgctgg atatagaaat tctaagggaa
     6061 cgttattatg ctaacaaggt atggaaacgt tcaagaaccc atgaagcagt agacagcgga
     6121 aaattatcgg gtacacatgg agatactgca aaaacttttt atatcggttc aaagaatagt
     6181 tctatctact tttgcttgta tgaaaaagaa aaggaacaaa aaagcaaggg tataaaaaca
     6241 gatattaaga accgcttcga aattcgttta aaaaatggaa aagcaggaca aacggtagag
     6301 caacttgttt tttcaagaaa tcccgaacag accatagcaa gctttattct cacacagata
     6361 gattttcccg attatatttt atgggatata tttttagata atgtaacgac ctcacttccg
     6421 tttattatga cacctgttgc tgttaatatg gataaaataa aacgctggct agaaagacag
     6481 gtcatgccct ctttattgat gataaaagag attgaaaaga aaacaggagc aaattatctg
     6541 gaagaaatcg acaggcatac aaggctaaca gaaaagcagt aattaaagat taaacaaatg
     6601 acaacagaca tagcagatat gattgagaaa gataccgctg ttccacaagg gaatgacggt
     6661 attttttaat ttcttcaaaa aaactgcaac aaaatgactg cttgtgtaca gatatggtgc
     6721 gaggaaagga aatctaactt tttttgaaaa caggtcatta aatcggtggc tgctgtccct
     6781 atatgatgtg aaagaagaaa cgatttttaa tttcaactta acatttgaca gttttcattc
     6841 cctatatgga gcaaagaaaa tcttttattc catagttggc agttacggtg ttgcatgggt
     6901 agttagggtg tgaaaagaaa aacagatttt tttcatcacc aacttagcaa actgatactt
     6961 gctttcccta tatggtgcag gaaaagagag tttaactatt ttcatttcaa cttagcaact
     7021 ggacgtttgc tgtacagata tgtagtgaaa agagaaaaag ttttgaatat tggttacgct
     7081 tcggctcttt tccaacgcgg tatataggaa agacgatttt cttctttcat gttctttgac
     7141 aactgaataa agcaattcaa tacgtttgac agacagcgat ccattgaagc agatacgcca
     7201 tgaccttttc cctgcaaaag cgagcgaacc ctatttgtgt tcctgtaaaa gacttgtcct
     7261 cttttatcgc catgacctgt tctgtctgat aaatgatact cccgtacagc cacagcttga
     7321 acgttcagag cgtcgcacgc aatgggtacg gctacataag aactatgcag aggtggaatt
     7381 cctgtgggct atgacaaatg ccgttgaatt gcttagaaaa aagattatgg aaagggggta
     7441 tgtgttattg ctgatattgt aaaaacaatg caagaaaaga caaataataa gcgtagcatt
     7501 ggcaacagag gaaaaacaaa gattgttgta aaagagcatt tttccgaaaa gggaaagaca
     7561 atggaggaac ttctgactga tgttatgctg gaaaaagcaa agcagacaat cgcataaaat
     7621 tggtgcagtt gtaagacata gattgaatgg caaaaagtac ccgtgttata ctttacttgc
     7681 aagcaggtat tgtaaacacg ggttagttat aaaggaggat aactgacaat gaaacaacag
     7741 atttacaata ctgcacttta tcttaggtta agccgagatg atgaattaca gggcgaaagt
     7801 tccagcatta ccacacaaag aagtatgttg cgtctatatg caaaagaaca tcatttgaat
     7861 gtgattgatg aatatattga tgacggctgg tcgggaacaa attttgaaag accgagtttt
     7921 caaagaatga ttgaggatat agaggcagga aaaatcaact gtgttgtaac gaaagacctt
     7981 tcccgacttg gcaggaacta tattatgaca ggacaatata cagaattgta ttttcccagc
     8041 cataatgtcc gttacatagc gattgacgac ggtgtagaca gcgagaaagg cgaaagtgag
     8101 attgcaccat ttaagaacat catcaatgaa tgggtggcaa gagatacaag ccgtaaagtg
     8161 aaatcggcat ttaagacgaa atttgcagag ggtgctcatt atggtgctta tgctccatta
     8221 ggatataaga aacaccctga catcaaagga aaactgctgg ttgatgatga aacaaaatgg
     8281 attgttgaaa aaatcttttc cctagcctat caaggttatg gtagtgccaa aatcacaaag
     8341 gtactgcgag aggaaaaagt tccgacagcg tcttggctga attttacaag gtacggtact
     8401 tttgcacata tctttgaggg aaagcccgaa agcaaacgtt atgagtggac gattgctcat
     8461 gtcaaggcaa tcttaaaaag tgaggtttat ataggcaaca gcgttcacaa tatgcagtct
     8521 acggtttcat tcaagagcaa gaagaaagtg cgtaagcccg aaagtgaatg gtttcgagtg
     8581 gaaaataccc atgaaccgat tattgacaaa gaagtgttct atcgtgtgca ggaacagata
     8641 aaatcaagac gtagacagac aaaggaaaaa gcaacaccga tatttgcagg gcttgtcaag
     8701 tgtgcggatt gtggctggtc tatgcggttt gcaacgaata cggcaaataa aacgccttac
     8761 agttattatg cttgcagtta ctacgggcag tttggcaaag gtacttgttc tatgcactat
     8821 atccgttatg atgtgttgta tcaagccgta ttgaaacgat tgcagtattg ggctaaggca
     8881 gtacagcagg acgaagaaaa ggtgctgaac aagatacaga aagcaggcaa tgcagagcga
     8941 atacgggaaa agaagaaaaa ggcaagtgcc tttaagaaag ccgagaaccg acaaaatgaa
     9001 attgaccgtt tgtttgcgaa aatgtatgaa gatagagcct gtgagaagat aacagagcga
     9061 aattttgtga tgttgtctag caagtaccaa aaagaacaaa tagaactgga acagcagata
     9121 accaacctaa gagaagaatt aagtaaaatg gaacaggata tgataggtgc tgaaaagtgg
     9181 attgagttaa tcaaggagta ttccgtacca aaggaactga cagcaccgtt attaaatgca
     9241 atgatagaaa aaatccttat tcatgaagca ataacgaatg aggataacga aagaatacag
     9301 gaaatagaga tatattaccg atttattgga aaagtagact gataaagagg gttcatatct
     9361 ttaactaagg gaaactggag aatctattcc tgatataggt agttgcatta aacttgcaaa
     9421 attgtacaag gtcaaactag atgacttagt taatcattcg gaagaaaaaa caggtattat
     9481 agtacctcct aaaggaaagt ttttctttgg agcagtagta gttggtgaac gtggtcagat
     9541 tgtcattcca aaagaagcaa gagaagtatt taatataaat gcaggagata aattattggt
     9601 acttggagat gaggaaagag gattaggaat tgttcatcaa cgtgatttga taaattttat
     9661 aggtcatatg ggagtagtac gtaaagaaga atgattttaa aaaattataa aaggaggtat
     9721 gaaatgtgag taaaatagta ttttttagca ttcttgcata tggtcatact aatcctacta
     9781 ttgaagtagt tcgtgaatta gtggatagag gaaatgaggt attgtattat tcttttaatg
     9841 aatttaaaga taaaattgaa ggtgctggag caaaatttat ttgttgtgat aaataccttc
     9901 cagaattact ccctggagat gagaaaaaaa ttggtaagga ttttcctgga ttaattgaga
     9961 tgatagtaga tactactatt tctttagatg aaaaagtttg tagggaactt aaagatttta
    10021 atccagattg cattgtatca gattcattaa gtttttgggg aaagttattt gcacaaaaac
    10081 ttaatattcc atatgtatgc tctacaacaa cttttgcatt taataaacat acagcaaagt
    10141 tgatgaaaca aaactttata gaaattatac gtatgatttt tggtgtaaga cgcattaata
    10201 aaaaaataaa gcttttacaa agaaaagggt atgaagtaaa aaattttatt tctatagttt
    10261 ctaatgataa tgatacggat accattgtat atacatcaaa agaatttcaa ccaatggtag
    10321 aaaccttctc tagtaagtat tcatttgtag gtccatcagt atcaaagttg attattaaac
    10381 ctaaagagag aaaaagaaag ttaatatata tttcgcttgg tacaattaat aataaaaatg
    10441 ttagctttta taaaaactgt ataaatgcat ttaaagattc tgatgttgat gtgattatgt
    10501 ctgttggtag aagtactgat attaaaagtt taggaaatat accaaataat tttgaggtta
    10561 aaaatagtgt tgaacagatt gctatacttc aaaaaactga tgtgtttatt actcattctg
    10621 ggatgaatag tgttaatgaa agtttatatt atggagtacc catggtatta tttcctcaac
    10681 attcggagca gagaatggta gctaagagag ttgttcattt gggagcagga attatgttaa
    10741 aagaggataa gtccgaaagt attaaaaaag cagtatttca agttataaat gacaatgaat
    10801 ataaagaaaa tgcaactaaa ctatcaaaaa gcttttataa tgcaggaggc tcaaaaaaag
    10861 cagcagatgt aatattacag attatatgta atagtagaca agttgacata taatagattt
    10921 gttaaaaata tatatacata catgcaattt aatcaaagta ctgacagtgg ggtcttttaa
    10981 cctaccagtt ggggcatata tattgatgct aaatatgagg gatgttatat tttaataaaa
    11041 aagataacat ccttttttta gtgcgttata tcaagtttta aataataaat gaaggaggat
    11101 taatatgaaa aaatataaag ttatttttat agtattaatt ataggtatta taagtgtaac
    11161 agggtttgtt gtatataaaa actgcattaa accatggaaa gatgctgaaa catatattga
    11221 taagtatatg gtaaaacaag atatttcaaa agataatata aaaagtatta caaaagaaaa
    11281 agccaagaag gcaagttacg aaggaatact atacaaagtg tattacaaag atgaccctcg
    11341 atatgagtat caatattttt atagtgataa ttatcatgct ctatatgtaa ataaagtaat
    11401 gttacaaatt tatgatgtgg aaaataataa taaactttta agaagtaatg aggaattaaa
    11461 aagtgtaaag tatccaccaa tatatcttaa atataagcca gataaatgat ttatgacaaa
    11521 atagaatgat ttttattaaa atttatttaa aaatatcact agagtgttta aataaaacta
    11581 aatttaagta tttatatatt agtatatctt actagtgttt aaacaaaatt aaatttaagt
    11641 atttatatat tagtatatct tactagtgtt taaataaaat taaatttaag tatttata
//
LOCUS       DAEPOZ010000118        11686 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.118, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000118 DAEPOZ010000000
VERSION     DAEPOZ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11686
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.118"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(381..806)
                     /locus_tag="KR440_002321"
                     /pseudo
     CDS             complement(381..806)
                     /locus_tag="KR440_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
     gene            complement(1279..1566)
                     /locus_tag="KR440_002322"
     CDS             complement(1279..1566)
                     /locus_tag="KR440_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699362.1"
                     /translation="MRLNVTKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNVFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(1586..1861)
                     /locus_tag="KR440_002323"
     CDS             complement(1586..1861)
                     /locus_tag="KR440_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699363.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAIGMLKGVDYEPLIVATQVV
                     AGTNFKFICNATSVTNPPHDYLAEIIVFKPLPCDEHNATITSINIIK"
     gene            2049..2868
                     /locus_tag="KR440_002324"
                     /pseudo
     CDS             2049..2868
                     /locus_tag="KR440_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418025.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(3127..5694)
                     /locus_tag="KR440_002325"
     CDS             complement(3127..5694)
                     /locus_tag="KR440_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBH0699364.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTNQLEDVSKKIGELFHNGEMPQEVSDAIKTAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDAVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMDGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIALQSEKE
                     LWGLSEWVKQNNSINLYLQNSPAIKIAEDFMSSVMPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAYSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     APQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFDE"
     gene            complement(5697..6302)
                     /locus_tag="KR440_002326"
     CDS             complement(5697..6302)
                     /locus_tag="KR440_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0699365.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPSDSSEKMLDETMNIFLHGIMNDGGE"
     gene            complement(6676..8136)
                     /locus_tag="KR440_002327"
     CDS             complement(6676..8136)
                     /locus_tag="KR440_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HBH0699366.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRANIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDRAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            8467..9045
                     /locus_tag="KR440_002328"
     CDS             8467..9045
                     /locus_tag="KR440_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0699367.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPPKEGFAKSLKMLYRLYDKN
                     PFLYSPNNPDFISLINKLPQDMFLQLESNNIQSFYDLIKRHNLKLKIEKEKAFGVCNS
                     LLSSLLVKENIEYNYIEVFDFMVDNLVEHILE"
     gene            9337..10587
                     /locus_tag="KR440_002329"
     CDS             9337..10587
                     /locus_tag="KR440_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0699368.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            10748..11317
                     /locus_tag="KR440_002330"
     CDS             10748..11317
                     /locus_tag="KR440_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBH0699369.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVDFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAVAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT         3834 a         2046 c         1634 g         4172 t
ORIGIN      
        1 gtaagcacag actatatctt atccttcggc attatccgct aaggtctacc cacttccact
       61 accaatagct tgtagtgtac ttccctcaag aggaatagtc gttgaagttt ctcctattcg
      121 gagcttacct gctgattgcc cattttaaag tacttagggt ttaaccttat accatctaac
      181 caattttttc tactttcgta actttcacac ttaggtttat ttcatcctta tgttttagtt
      241 tggttagctt taggggtttc cagcaattca agtagtattg gataggctct atcctatctc
      301 tacatatagg tttccctata tgctgactat tttagctact aactcatgac taaattcacg
      361 agtgtgcgta gctatttttt aatcaataca attactgcac atatagatgc agaattttta
      421 aatacttcac ctaatgaaac tgctgcacta atatcatggg ctactcccct attgtcgcta
      481 aaacctgcag tatcacataa ttttgcatcc attccttcta ttggttttct catatttaca
      541 atgtttgggt ataaagtttg agaaacagct cctgaaccaa tctctgccac agggtctaag
      601 caatcaatat atgatatacc agtatcttca tcttctactt ccatcattac acatcctgca
      661 agatagttaa ttgcagtact tttgccacat cctgtcccac caagaattag aattaaattt
      721 tgattttcag ccttatttat tgctactttt ccttcattta ataagagtgt tatttcttct
      781 aaaagttgat tgttattttc tggcatttat tttccccctt ggttatatat tttatttaaa
      841 taaagtttaa tcccttaagt agattacaaa gtttttagac tatatttaaa tttgtttttt
      901 atttttaata aatttgcatt ttcactcaat aattattctt ctttgtttat taaaataact
      961 gtgttgtata atataatttt taccaacaca gttattttat ttaatgcgct tattttattg
     1021 tattttatgt aatacagttg tttttttata ttatatgtcc actattttcc tatggttatt
     1081 acatatagat ttttaattat atttgtacca ttaatttaaa cattcctata ctactgctta
     1141 agttttacac ctataccagc ttgaatattt tatttttatt tgcttaaata gttcaactat
     1201 actggtttaa taggtttcct ttagtttaaa tagttcacct atacctactt gaatagttca
     1261 cttttatttg cttaaattct ataccaatac tggcttaaac attatactac catgattgta
     1321 ttgtctatct tcaactacaa taaaaacagg ttctaaatct ttagtagatg ttatttttaa
     1381 ttctaaaaaa acattatcag ttggtaatat ttttacatca taattattta aattattatt
     1441 cgtgatacta ttaaatacat caccttgacg ttcaaaaaaa acattatcaa ctattacaaa
     1501 ataaacatct tcttttctac tattttcaac ctctatagta ttttgtttag ttacatttag
     1561 tctcatcttc cacctcctat taatattact taattatatt aatacttgta attgttgcat
     1621 tatgttcatc acaaggaaga ggtttaaaaa caattatctc tgctaaataa tcatgtggag
     1681 gatttgtaac tgaagtagca ttgcaaataa atttaaaatt tgttcctgct acaacttgag
     1741 tagcaacaat aagtggttca taatcaaccc cttttagcat tcctatagct tcattaaata
     1801 cttttaaatc ttcttcttcg acagccctaa attttgacca tccaccaaca aaaagactca
     1861 tataaaaacc tcctttatat atatatttat ttatatgata gtaccaattt tcttaatatt
     1921 caatatatta cgtctaactg gtcaataaaa atgcataact ggataaaaag acttataaaa
     1981 tagcttaact ttacatataa taagattatt ctaaaatatg agacatatct tgctttgaat
     2041 atattctata caacaatttt ccaggcctca ttgccaagtt taaggtaact gactttggat
     2101 tccaacttct tgatgctaat aaaaaatatt ttgatatgtt caaagataat gtaaattttc
     2161 tattagatga tataaattta gaaggttatc ttaacaatgt gtataaatat cataatgatt
     2221 ttaaatctgg gagatgcgtt tgtttttcta tttctccaca aactattaat ggagatattt
     2281 ttcacatgca agttgtagct gaatgtatag attgggaaga tgataagcct atatatcttt
     2341 taatatattc agatgtaacg acattggtaa aacaatatgt ctcattatca tttttcatcc
     2401 aagaatttta tcaagaaata tgataaaata gctaaaaaat atgatattcc ttctcacctt
     2461 atagaaattg agattacaga aacaattgta tttgaagatg tcaaagtatt tagtgaaata
     2521 ataaatgaaa ttcataaaaa tgactttaaa tgctctattg atgattttgg aagtggctat
     2581 tcttcaatca atatgcttaa gggtatttat gttgatacat taaaattaga tagagcattt
     2641 tttcaatcag aagatttaga ttctcaacgt gaaagaaata taattgtatc tataatcgaa
     2701 ctagctaaaa agttaaaaat gaccactgta gctgagagaa tagagaccag ttttcaaaaa
     2761 gatttcttgc aagaaataaa ctgtgatatg ttacaaggat acgtgttctc tagacctttg
     2821 caaataaaag actttgaaaa acttatgttt caaaactcct ccaattaaca atataaaatt
     2881 gcttattata agtgtattat tgtatcaaca aataaaggaa ttgtctcaaa attgattttt
     2941 tctaatcaaa tttttatttt gagacattct catttttttg cataagaaaa tacccaccaa
     3001 gctacggttt ttcttaccgc tgttcggtgg gtattagtag tattatgaaa tctgaaaact
     3061 ttaagtatta ctacttccgc taacgcaatc acaaaggtcg aaagcttcaa agcgaaaata
     3121 ggctttttac tcatcaaacg tcaccgtacc tgccaacccg tccaccgtta tcatttgtcc
     3181 gctcttaata gttcgtgtgg caatgtgtgt agccataacg gctgggatgc catattcacg
     3241 tgcgacaatg cttgaatggc taagaggacc accaatgtcc gttacaatcg cacttgcaga
     3301 tgaaaataat ggtgtccatg ctggagttgt ggtaactgca accaaaacac ttccaggttg
     3361 aaagctttca aaatctgctg gactattcag tacacaagct ggagccgtca caacaccagt
     3421 actggtacca atgcccgtca acacaatttt cccatctttc tgcttctgtg gtgcttgtga
     3481 tatagttttt ttgtttttct ctggcaactt ggacggtgac acataaccca tgtatttttt
     3541 aagttcagct tttcgaacag gtattatccc gttcatatca gatagagact tgtttttatc
     3601 caactgctct ataagtacct ccaattccga ttttgtgagc caataaatat catctaaatg
     3661 tgaaatagct cctcctcgta tgaatcgttc tgagatttcc tgaaacatac aacgaatcag
     3721 aggatgtccc attcccatca aataaatagc attttcacgc ataggagagg tttcctgtgc
     3781 ccaatggagc aacttaaaaa atagtttttt acgcaatcca cctatgtgtt gtaaaatctc
     3841 ttcttctgca tgttttctac gcttttcaaa cgtggtctgg cgtaaaaaag gactttcccc
     3901 ttttccttcc acaaatgttt ttacggattc aaaagttggt gtaagtgttt cctgcggtgt
     3961 ggaatacgca aaatcaaact cataagcggt acgaccaaac tctttaaagt acttattgat
     4021 tcgatttttc cattctatcc atacttcctg tgaaactcca acaggcataa ctgaagacat
     4081 gaaatcttct gctatcttta ttgcaggact gttttgcaga taaaggttga tgctgttatt
     4141 ttgctttacc cattctgaaa gaccccacaa ctccttttcc gattgcagag caatcgtatc
     4201 aaacccaagc aggaaaacgg aagtatccgt tataccagca cgtcgaactg ctccttgaaa
     4261 taattttgta aataatgttt cactgataga agcagcaggc aatgtaagct gaatccttgt
     4321 gaaataaata catgcggcat aaaatattgt ctgaatacca tccataattt gatgggcgtt
     4381 catcgtatgc aggggctttt cttcccattg tttaatagca tcctcatatc cttttcgtgc
     4441 cgtttcccag cgtgcaacgc tgttcataag tgtcctacgt aaagagcgtg gggaaaggga
     4501 tttgatagca atcaaaagtg gctttgattt agcagaaaga taaacatatc cattaataac
     4561 cgtatatgcg ccgttttttg gtaataattt tttcgcactt ttaccaaaca tatctatcca
     4621 taaaagctcc gatgcacggt ttacgatttc aagaccaagc gtggcgaaca aaggtgtaac
     4681 aggatttggc aagtgttctg ccaaacttcc ctttgtgtat acaacatcct gttctggcaa
     4741 tacccattcc ggtggcagaa cagtaattgg acgagcttga acaatgtaca atttatcttt
     4801 ttccagtgcc cactctacat ccataggcat ttgataatac ttctcaattt tctttccaag
     4861 ctgtatcagt tgcattacct gattacgagt aagagcatat ttttttctca acctttcagg
     4921 aaccatggtt tcttctgttc cttctgaagt acgaaccgtc ataatctctt tatttgccac
     4981 ttcatacgat ataattcgtt cggagtcctt atccactacg attgtatcgg gtgtaactaa
     5041 gctagaaacc accgcttcac caagccccca tgcagcgtta ataatcattt cgctacgtct
     5101 accattgatt gggtttaacg taaacataat accagatgaa tcggaaaatg caagcttttg
     5161 tactacaaca gcaagtgcga caatctcgtg ctttatatta ttcttcacac gataagcaat
     5221 agcacgagct gtccataagg aagcccagca tctctttaca gcgtcaataa ctttatcttc
     5281 accttgtatg ttaagataag tttcctgctg acctgcaaaa gaagcgtcag gtaaatcttc
     5341 agcagtcgca gacgaacgaa cagccaccga tatgttccct aatccggcat aagctgtttt
     5401 aattgcgtcc gacacttcct gtggcatttc tccattatgg aaaagctccc ctattttctt
     5461 agatacatct tcaagttggt tggtattatt ggaatcaata ccatctagca gtttattaat
     5521 tcgagactga atatggttcg tctcaacaaa aatctggtat gaagcagtcg taacatgaaa
     5581 accgtctggc acagggatgc ctgctgtcaa tagttttgat aaagacatcc cttttccacc
     5641 aacaatttca atggttgctt gtttatgttc cagtggcaag acataataaa tcataatcat
     5701 tcacctccat cattcataat tccgtgcaga aatatattca ttgtttcatc caacattttt
     5761 tccgaactat ccgatggtcg tgacgtgtag ctgattgata acatggagtt aattaacaaa
     5821 cgtgtcacaa gagcggtatc cgttttgcga aagatgcctg tggtcacacc attatcaatt
     5881 accgatttta taatatcttg atatgcataa cgagcatctt taagtctctt tcgatactct
     5941 tcttctaaat aatcagattc tgtatttaac cacatcagta cagttctata ctgctctgga
     6001 aatctaagat gattaagcat gatttttcta aggcactgtt gtggtgaagg ttcaccatca
     6061 ataatcctat gaacttgttg gatagttttt tctatttctt tctcaactgc atatacaaca
     6121 atctcgtctt tcgttttgaa aaaatcgtat agacttgact ttcccatgtt ggctaaaact
     6181 gcaatttctc tcattgatgt tttttggaat ccattttcag ccatgagact caaagcaaca
     6241 gcaactattt tttctcggcg ttctgcttgt tgttcaggtg taagcacaat tccttttggc
     6301 atagggcgat acctcctttt agcgaccaat ggtcgtttct tttttattta tcatacagcg
     6361 accatcggtc acttgtcaag gttaattttt ttcattgtat atggtactcg agataaaaat
     6421 gttcatttta gaaattgctc tatttaagta gaaataggct tcatcattag aaaacaagac
     6481 tataaatttg agatatttat catgttcaaa tttcatcaat atcaaagttt aagatattaa
     6541 taaaaaaaga attactaaga aataacatat aaagattcaa aaaaggagtt gaacttaaaa
     6601 ttcaattaca aaaaatcaaa tttaagacaa ctcctttatt ctttatctct actattcttt
     6661 atctctattt atttattaca actccctaac atttaccact actgccttag gtctagtcat
     6721 agcctcgatt gaatacttaa caccttgagt tcccatacca gatgccttta cacctaaaaa
     6781 tgggaagtgg tctggacctc tttcagtctt attatttatt tgaacagtac caacttcaag
     6841 tttatcagct atataaaatg ccctatcaat atcacttgta aatactgatg attgaagccc
     6901 atactctgat tgattagcaa tttcaattgc ttgatttata tctttaactc ttataattgg
     6961 aagtactgga ccaaatggtt cttcccaagc aattttcata tcaacattta cattatctaa
     7021 caatgttgga tagaataaat tattttttct aacattacct gttattaact ttgctccttt
     7081 atgtaatgca tcatcaacca acccttgaac aaaatcagtt gctttattat ctataagagg
     7141 agtgataact acttcatcca ttggatttcc aattgtcaac ttctctacca atggctttat
     7201 ttttccgact aatttatccg ccacactctc ttgtactaaa attctcttta ctgccgtaca
     7261 tctttgtcct gaatatgaat acgctccaga tactatattc ttagctgcaa aatctaagtc
     7321 tgcatcttct aatactatag ccgcatcttt tccaccaagc tccaataata gtggtaccat
     7381 tccagatatt ttagatatat gctgaccaac ttcagtacta ccagtaaagt taataaaatt
     7441 aattccttta tgcgttacaa cataatcccc tatctcacta cccctacctg taatagtatt
     7501 aagtactcca tctggtatcc cagcttttct catgatttct accacatgaa gtgcgctaat
     7561 agccccttgt gtagcaggtt ttaatacaac tgcatttcca ccaataagcg ctggagcaat
     7621 ctttgacatt gataaattta caggataatt aaaaggagat atagctaaaa cagtacctaa
     7681 tggaaccctt gaaacataag acatcttatt tcttgttcca ccagggaaat tatctccact
     7741 aatggcttca ccctccatat tttttcccac atcagctgtg tatcttaaaa aatctgctgt
     7801 tctctcaact tctgcccttg cagattttat gtctttggct atttcaagta ccagtatatt
     7861 agctatctca tctatatttt ctaacaataa atcagcagcc ttatgaaata tattggctct
     7921 cttgtataca ggcatttcag cccatacttt tatactctct tttgtgtttt ttataacctc
     7981 atctacctca tgttttgtca tagattgtac tttcccaaca agagaattat ctattggtga
     8041 gtaaatttct attggctttc tcgaattaga ttctacccat tttccattta ttaaattttt
     8101 atatacattt tcttttgttt taagttcatt aaacatattt ttattcctct ctctgatata
     8161 actttgtgta tttaaattat agccatttat tcgttatgta aacatttctt tttttcataa
     8221 acgtttacct ataaaaaaaa ttttaagtta acgtttttct agattcttct attttaataa
     8281 catactcgct ccaaatctat ataggtcaca ctttaatatc aatataccaa tataccaata
     8341 taccaatata aatattataa acataaatca cttgacaaat ttataagtag acaatattat
     8401 atatctatga cttaaaatat ttttaaagtc atagatagca tatgcagata gaaaggattg
     8461 ataaaaatgg ctaaaagttt tacagaaata gaaaaagaca atataagaaa aagactaatt
     8521 tcagaatgtg aaatcaactg gtctaaatat ggatacaaaa aaactaacat tgatgaactt
     8581 tgttcaaaat caggaatcgc aaagggttct ttctatacat tttttgattc aaaagaaaag
     8641 ttattttttg aagtactaaa tggaatccaa aacagactta tatcatcagt agatgaaatt
     8701 ttaagtaaca tgcctccaaa agaaggcttt gcaaaatcct taaaaatgtt atatcgatta
     8761 tatgataaaa atccattttt atattcacca aataatcctg atttcatcag cctaataaat
     8821 aaactacctc aagatatgtt tttacaactt gagtccaata acatacaaag tttttacgac
     8881 ttaattaaga gacacaatct caagttgaag atagaaaaag aaaaagcctt tggtgtttgt
     8941 aattctctac tatcaagtct attggtaaaa gaaaatattg aatataacta tattgaagta
     9001 tttgatttta tggtagataa tcttgtagaa cacatacttg aataacaatt taaaataatt
     9061 tttctaaata tttctttata ctaaagagga ttaatatgat tgaattaatg aaaaaaataa
     9121 acaatatcat ttacaaaata aatgataaac taaatataca cacaaaactt atagacccca
     9181 gagttaactc attccactct actctaagcc atctataata gttcgtatat ttctaataat
     9241 attagatatt aaagtaagaa aaaaattcct tataataatt gctattatat caattattat
     9301 aaatatttta ataatcttag ataggtaggt atatatatgg taaaatcctc aaacactttt
     9361 aataaaaact tcaacttgat gatattaggt caaataatct cattgtttgg tgcatcaata
     9421 cttaaatttg ctttatcttt atacatactt gatataactg gtaaagcaga aatatttgct
     9481 actatactag cagtatcttc aatcccaatt atagtattct cccctattgg tggagctatt
     9541 gcggatatat tcaacagaag aaacttaatg gttatttttg atttttcaag cagtcttaca
     9601 gtactaatct tagcagtatt cttattcaac aataatggtt caatattact tgtaggaatt
     9661 attatgacca tattatcagt tataagcaca atgtatcaac caactgttca gtcaagtaca
     9721 ccccttttag ttgataatga gcatttaatg aatgcaaatg gtattgtagc tggagttgca
     9781 tctcttacaa atattgcagg acctgtatta ggaggtgtct tgtatggtgt gattggaatt
     9841 aatgcaatta tagttataag ttgtgtaagt ttctttctat ctgcaattat ggagatattt
     9901 atacagattc cttttatcaa acaagagcaa aacagcaaca ttataaaaac tatatttact
     9961 gatataaagg atggtattgt tcatatatgt aaagaaaata gatttatact aaaaatactt
    10021 tttttagcta tagcaataaa cttattttta agctctatgt taatagttgg tatcccctat
    10081 atagtaaaaa tcactatggg agctagtaac ttcttatatg gtctagcaga aggagcaatt
    10141 tcttttagtt cactggtagc tgctgttagt attggtattt tctcaaaaca tctaaaaatc
    10201 actaatttgc acatttcctt tattatatgt gcagttatgt tcataccaat ggcggtttca
    10261 gtatatccat ttatattaaa cacaggattt ccagttccat ttttaatctt tatagtatca
    10321 tctatgctaa tattttttac aacgactctt atctcaatat atgttataac agtcacacaa
    10381 ttaaaaactc caaatgaact cttaggaaaa gtaatgtcaa ttctttttgc aggaacagca
    10441 attgcaatgc ctcttggtca ggttatgtat ggtaaacttt ttgatttatt atcagataga
    10501 gtgtacatca ttatactagg agttggattt attacactac ttatagcatt tgtatcaaag
    10561 ataacattga ggaacgaaaa tctttagata caaagaattt agatataaag atttaatttt
    10621 cactaatttc tgaatttaaa tgcaaaattg cgtatcagta tgttgacgtt ttagaaattg
    10681 attgatataa tcaatggaaa ataaacttta taaaaattca aaatattcag ctagaggagt
    10741 gataaaaatg agttacatgc caactttaga acaagcatgg gaaatattaa gaaaatacaa
    10801 taaggaagaa tttcatataa gacatgctca aattgtatct ggagttatga gatactatgc
    10861 aaaggagtac gaccctgaga gagtagactt ttgggaaata gttggacttt tacatgattt
    10921 agattttgaa atgtatcctg atgaacattg tgttaaacaa agagaattaa tgacagagct
    10981 tgatttagat aaatcaataa tcgattctac aataagtcat ggatatggat taactggttc
    11041 agatgttaaa ccagagcaat ttatggagaa agtattattt gctgtggatg aacttactgg
    11101 tttaattggt gctgttgcaa taatgcgtcc ttcaaagagc gtttctgatt tagaattaaa
    11161 atctgttaag aagaaattta aagataaatc atttgctgct ggctgttctc gagacgttat
    11221 aagaagtggt gcagaaatgc ttggatggga acttgataag cttatccaaa caactataga
    11281 agctatgaga agcttgattc cagagatggc aatatagctc ctcatacata taccatagaa
    11341 gttacatctt taaagagcat ttctcttttt gatgcaactt ctttttatat tttgtagaat
    11401 aaaaagttta atattttaaa ctcactatat aatctataat aaatactata aataatataa
    11461 tgtataatag tcataaactt tctaacctta aaagtttcta atactcaata aacaattcta
    11521 atatacaagt ggcattatat gatttaatta tatatcttca atttatatat taaaataaat
    11581 atatatatta aattcttata caccaaattt ttacattaaa cttctatgca ccacatttat
    11641 acatattaaa ttcttatata ttaaatttct atgcatcact tttata
//
LOCUS       DAEPOZ010000119        11684 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.119, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000119 DAEPOZ010000000
VERSION     DAEPOZ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11684
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.119"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..276)
                     /locus_tag="KR440_002331"
     CDS             complement(<1..276)
                     /locus_tag="KR440_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699370.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFMPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKEFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFL"
     gene            complement(269..658)
                     /locus_tag="KR440_002332"
     CDS             complement(269..658)
                     /locus_tag="KR440_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0699371.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            850..1296
                     /locus_tag="KR440_002333"
     CDS             850..1296
                     /locus_tag="KR440_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699372.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEDIDLHEDAKKDNIYKEDEYKKYEEE"
     gene            1560..2285
                     /locus_tag="KR440_002334"
     CDS             1560..2285
                     /locus_tag="KR440_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0699373.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCKYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            2272..3516
                     /locus_tag="KR440_002335"
     CDS             2272..3516
                     /locus_tag="KR440_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0699374.1"
                     /translation="MFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMVVRN
                     NQAIIGTLSSKYPNLESQIVDIITQGKSMKNIDYGQKILSKYNYDKSIKINSEPIISK
                     LVLETIKINIILVCIIFILIFVLVVRYFTSIYNDLSDMTKYVYYSSEGKPFDMKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAML
                     SMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNGDIY
                     VNSEKGKGTEFHIIFHKMYDSD"
     gene            3596..4273
                     /locus_tag="KR440_002336"
     CDS             3596..4273
                     /locus_tag="KR440_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0699375.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            4274..6835
                     /locus_tag="KR440_002337"
     CDS             4274..6835
                     /locus_tag="KR440_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0699376.1"
                     /translation="MNLYTSLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDQNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIKKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVRNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINSSI
                     KSIIIAVTILVIICTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIIIIVSIIIVLLTIF
                     ISILLPAISASNISPLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGESYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAIEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGFGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNDFNFYDEISSSESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENIIDNIRRESI"
     gene            7056..8330
                     /locus_tag="KR440_002338"
     CDS             7056..8330
                     /locus_tag="KR440_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBH0699377.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
     gene            complement(8491..11532)
                     /locus_tag="KR440_002339"
     CDS             complement(8491..11532)
                     /locus_tag="KR440_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HBH0699378.1"
                     /translation="MFKQRISKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTTENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKVDDNNISNSVKEVWKKRSGKGYYLVDEKYTSQSYMFGSVKASFSLSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSAFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGNTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
BASE COUNT         4517 a         1372 c         1781 g         4014 t
ORIGIN      
        1 taaaaaacct ataataccaa taaaatatga aagttccaat ttattaaatg taaaaaccag
       61 aataaaaact aatataaagt ctataataaa acctaaagat aatagttttt taaattcttt
      121 atctaatttt ataaagtaat tataattttt atgctcttgg tctatagaaa ctccatagat
      181 gtgtttttta cctacaaaat agggcataaa ataaaatgta gcatacatca aaattagaac
      241 tggaaatatt gtaaatagac attcaatctt actcataata tttcacctca tacttgtcaa
      301 ataaacttga acttagtgac aaaaattctt ctttactaat acctgataaa taagattttg
      361 ccaccaataa ttccaagtca agtttatttt tttgacgagt tgtattatct attttcattg
      421 gtagttgagc tacaatagca ccttttcttc tatcaatatt taagaatccc tcttctttta
      481 actcattgta ggatttgttc actgtatgta aatttacacc aatattttct gccatacttc
      541 ttacagatgg aagtgattca tttattttca attctcctga agctatagcc ttagttattt
      601 cttctttaat ttgtacataa atagatttat cactattaaa atctaaattt aaaaccataa
      661 aaaacctcca ttatgcttta taaatgaaaa cgattgttat atacatagta taacataaaa
      721 aataataaca aacacaaaat ataagtttat attttgtaaa aattgtaatt taaaaaataa
      781 cataattttt attaaaataa tacagatact atttatagat agaaataaaa attagaaagg
      841 agaaaaaata tgttaacata tttaatgaaa agacaaatgc ataaaaaaga tgatggaatg
      901 agtaaaccaa tgatgattac aggagctgta attacaggtg taggagcata tgctgtatac
      961 aaaatggtaa aaagccataa atctaattct caaatggtca atacaaatta ttatggaaat
     1021 agcgattatg atgattataa atatgacgaa tttgattatg actgtgattg tagagacaca
     1081 aaacaagatt atgaatatga agaattaaaa aaaaaagtaa aagaatttaa ttcaagaaga
     1141 ataaattgtg aaaattgtcc acatgtttct caagaagaaa tgatgcaata tgtaaatagt
     1201 gttaaagatg taaaggaaga tattgattta catgaagatg ctaaaaaaga taatatttac
     1261 aaagaagatg aatacaaaaa atatgaagaa gaataagtaa tattttgtat aactgaacaa
     1321 atagtaatgt caaattattt aatatctagt tttaatatgt aagttgtaac ttaacttgaa
     1381 agaatcagta tcattcttta gatagttaag ttacaactta cattttgttt ttataaattt
     1441 taatagttct ttgtgactat tttgtaattt tagaaatatt ttagtatcca gggtaattgt
     1501 gtataataaa acttgtatag atagatagta tgaaattaag cagatagttt aaaattaaaa
     1561 tgaaaagaaa ggaaatagtt atgttaaagt tgttgatagt tgaagatgat agtactatat
     1621 cttttggaat aaagtatgct ttagaacaag aaggatttag tatagatata tcaaaggatt
     1681 tatctagtgg aaaagaaatg atttcttcta atgaatatag catgatactg ttggatgtta
     1741 cattgccaga tggaactggt tatgagcttt gtaaatatat aagaggattc tctcaggttc
     1801 ctataatatt cttgactgct tgtgatgaag aagtaaatat tgtaatgggt ctcgatatag
     1861 gtggagatga ttatataaca aaacctttta gaattagaga attaatatcc agaataaaag
     1921 ctgttttaag aagaaaaggt aatacttcag aagaaaataa gaaaatactt aaatttggag
     1981 atttaagtat atatacatta gaagctagag tatataaaaa tgataaagaa atatttctga
     2041 catcagttga atataaactt cttttaattc ttatacagaa taaaaatact gttttaagta
     2101 gaactcaaat attggaaaaa ctttgggatg taacttatga ttttgtaaac gacaatactc
     2161 taactgttta tataaaaagg cttagagaaa aaataggtga tgatttatgt gaaccaatct
     2221 acatagaaac tgtaaggggg attggttata aatggatagg aagtgaaaat agtgtttcta
     2281 tataatccag aagtgaagaa gtttttaagt aagtatgtta ctttgatgtt tattgttatc
     2341 ataatatcta ttggatttag cataatcaac gtgtctttaa ctaaagagat ggttgtaaga
     2401 aataatcaag ctataatagg gactttatcg agtaagtatc caaacttaga aagtcagata
     2461 gtagacataa taactcaagg gaagtctatg aagaacatag attatggtca gaaaatatta
     2521 agtaagtata attatgataa atctattaag ataaactctg agcctattat ttctaaatta
     2581 gttttagaga ctatcaaaat aaatataata ttagtatgta taatatttat tttgatattt
     2641 gtgttggttg ttagatattt tacgtcaatt tacaatgatt tatcagatat gactaagtat
     2701 gtttactata gttcagaggg taagccattt gatatgaaaa acaagaatca agaagggcag
     2761 ataggtcttc taaaaactga actcttaaag atgacgacaa ttcttaatga aaaggttgaa
     2821 cttttaaaaa gagaaaagat atttttaaac aatacaattt ccgatatatc acatcaatta
     2881 aaaactccta tgacttcttt aataatgttg aatgatttat tatataatga tgtaccatac
     2941 gaagtaaaaa tcgatttttt aaataagata aaaaatcaat taaatagaat ggattggttg
     3001 attaaaagca tgcttaaatt atcaaaggta gaagcaaaag tgataaattt taaaaaagat
     3061 aaggtaaaat ttagtgaact catacataga gcaatgctat ctatgaaaat acctatggag
     3121 attaaaaatc aaaaactgac tatagaggga aatgataatg tatcttatat tggtgatatt
     3181 gattggtctg tggaagcact agtgaatata atcaaaaatt gtgtagaaca tactcctgaa
     3241 tttggaaata taattataac ctataaagaa aatccattat tttctgaact aataataaaa
     3301 gatgagggtg aaggtataga taaaaaagat ataccacatg tatttaaacg attctataga
     3361 ggaagaagta gttcaaaaga agatagtgta ggaataggtc ttgcaatgtc aaaatctata
     3421 atagaaagcc aaaatggcga tatatatgta aacagcgaaa aaggtaaggg tacagaattt
     3481 catataatat ttcataaaat gtatgatagt gactaatctg taatatttaa ttcactttat
     3541 agtaatagcc aaaacctaat atatgaagta tagaatataa atattggagg tagttatgga
     3601 aattttaaga gtagaaaatc taactaaaag ttatggtaaa aatgaaacaa aagttgatgc
     3661 tataaaaaat gtaagtttat cagttgaaaa aggaacgttt atagcaataa caggtccaag
     3721 tggaagtggt aaaagtacct tattacattt attaggagga gttgacagac caactagtgg
     3781 taaggtttat ataaatgatg ttgatatata taatttaaaa actaaggatt tggcgatatt
     3841 tagaagaaga aatataggtc ttatatatca attttacaac ttaattccag tcttaaatgt
     3901 gaaggaaaac atattgcttc cagcagagct tgacaataga gatattgata gagaatattt
     3961 tgatgattta atgaaaacat taggtctaat tgatagagaa aagcaccttc caaaccaatt
     4021 atcaggaggt caacaacaga gaacctctat aggacgtgca ttaattaata gaccttctat
     4081 aatattagca gatgagccaa caggtaattt agatagtaaa aattcaaagg aaatattaga
     4141 attgttaaaa ttgtcagtaa aaaaatacaa tcagacacta ataatgataa ctcatgacaa
     4201 aaatatggca cttcaagctg atagaattat aagcatagaa gatggaatca taaaaagtga
     4261 tgaggtgatg tagatgaatt tatatacatc actaaccatt agatacttaa aacaaaataa
     4321 aagaagaacc attgtaacca taatcggtat aatactttca acagctttaa tttgtggtat
     4381 aggaaatata tttgaaagtt ttatggatta tcaaatgaga gaaactataa agaatgatgg
     4441 aagtttccat gttacatttt atgatgtaaa tagaaaaaat gtggaatatg taacgaaatc
     4501 agctgaaata gaaaaacatg catttaccaa acaacttgga tattctaaat tagaaaatag
     4561 tgaaaatgct atattaagta taaagcaata tgatcaaaat gcaataaacg gatataaagt
     4621 ttctataaaa gaaggtagat ttccagctaa ggaaggagaa attgttttaa gtgaaagtat
     4681 tattaatctt atagatgata agttaaaaat aggagataag ataactttaa aagtagggga
     4741 tatgtttgac tcaagcaaga aaaagattga tagtatgaca tttcatgaag gtgattatat
     4801 agcaaatgaa aaagatagaa cttttaaagt tgttggtata attaaaaaac ctgggtttga
     4861 acgttataat ggaattgcaa cagctatagc ctactttaat cctatgaata attacaatga
     4921 tacaattaat gtatcagtag cagtaagaaa tcctaaagat gtgtataaga taagtaataa
     4981 aataagtaaa aatatatatt caaataaaga taaagaagca gtttctgata tgataaaata
     5041 caatgaacat ttattgaggt tacaaggtgc tagtaagtat tcaaatataa atagctctat
     5101 aaagtctata attatagcag taactatttt agtaatcata tgtacaattg ctacagtgta
     5161 caattccttt agtatttcta taaatgaacg aaaaaaacag tttgggatat taaattctat
     5221 aggtgctaca tctagtcaaa ttaaaagatt agtttttata gaaggtatta taattagttt
     5281 aattggtata ccaataggac taatatctgg aacagtagct atagacctat tgtttaaaat
     5341 tataaataag tattttactg aatcggttgt tactaagatg agtttgcaga tagtgtataa
     5401 tccaataata attattgtta gtataataat agttttgctt acaatcttta tatcaattct
     5461 actacctgca atatcggctt ctaatatttc tccccttaat gtgattaaaa atactggaga
     5521 gtataaagtg ggcaaaatca aaagctcaag acttattaaa atgattttta aaactgaagg
     5581 agtacttgca tataaaaata ttaggagaaa tagaaaaaaa tttataataa ctcttttttc
     5641 tttaatggta agtataataa tatttatatc ttttagtggc tttacattac ttttactaag
     5701 aggtgaagaa attagaaatt ctcaaagaaa atatgatttg tatttaactg caaagaacac
     5761 agctaggtct gtagaggata ctataagtga actagaagat attgatggga taaaaaactt
     5821 tgaattggca acaggacaat atgtgtctat aagagtaagt gaaaataaaa taaataaatc
     5881 caaggaagat ttaatcagga aatattatca aaaatataag attggagaaa gttatgaata
     5941 tgatttcagt aataatgaat tgatttttcc gggagatttt gcagtaaaaa acaaaataaa
     6001 taacttagta cagggctctt ttaataaaga gagagcaatt gaagaaaatg gtgttatatt
     6061 agttagaaaa agtgcaattg aagaaaatgg gaagaaaggt gtagtagaac ttaccaacta
     6121 taaagtagga gatacagtaa attgtgaata tcttgatgaa aatgccttaa gtaaaaaagt
     6181 aaaggtcaag attcttgcta taactgatga agaacgtcta ggttttggtt atcaaaatat
     6241 ggggttacaa tttataactt atgatgaagt tgctaaaaat ttaaatttaa aattaagtaa
     6301 aagccttata tttattgatt caggaggaag cattaagact aagaaggaaa tagaagcttt
     6361 ggcaaataaa aatgatttta acttttatga tgaaatttca agcagtgaaa gtgaaaaaca
     6421 agatttaaag gttattaaaa tatttgtata tggttttata gtagtgatat ctttagtgag
     6481 tgttacaaat attctaaata cagtaagtac aagtataaat ctaagaaaaa gagaacttgc
     6541 agtaatacaa tctatagggg ttactcctaa aggatttagg aaaatgatat atttagaaag
     6601 ttttatatat ggtatactgt cattgttgtt tggaatacca attagtattg gtataattct
     6661 aattatgaat aaacttattt caggtgtaat tgaattttct ccgattatac cgtggacagc
     6721 catagttatc tgtatagtaa gtgtttttat aataactttt atagcagggt atataccaat
     6781 gagtaaatta aataaagaaa atataataga taatataaga agagaaagta tatagatata
     6841 aaaataattc ttaactatat aaaatattat taaaatcatt taagtggtat aaaatattat
     6901 tatatcactt aaatgatttt ttatttatat aagaaatcta ataataaatt actagaggta
     6961 tttaactatt tagaaagaat tggtattata taaagtaaga gaatatataa taagaaagaa
     7021 tatttgattt tatttgaaaa caaaagaaaa agaaggtgat tactatttct catataactg
     7081 cgaagaatat gtataaaagt ttagaggaaa gaataaacaa gtttccacaa ggtgctccac
     7141 catctgatac attatacaaa atactaaatg tactatatac agaacaagag gcgaagttag
     7201 ttgctcagct tccaataaag cctttcagag taaaaacagc agctaaaatt tggagtgtaa
     7261 gtgaaagtga agcatataga gtattggata aacttgctag taaggctctg atactagata
     7321 tagaagataa taaagggaag aaatatataa tgcctcctcc aatggctggt ttttttgagt
     7381 ttgccatgat gagaacgaga cacgatatag accaaaaact tttggcagaa ctttactatc
     7441 aatatatgaa tgtagaagaa gattttatca aagatttatt ttattcaaca gaaacgaagc
     7501 ttggaagagt atacgttcaa gaggaagttt taactaatga taatgaggtt agtattttag
     7561 attatgaaag ggctactcat ataattgatg aatcaactca tataggtata agtatgtgtt
     7621 actgtagaca tagaatgcaa catgttggaa aagcttgtga tgctcccatg gatatatgta
     7681 tgacgtttga caatgtagca aattctttaa taaataataa atttgcaaga cgtgtggata
     7741 aaatagaatg taaagaacta cttcatcaag catatgaaca taacttagtt caatgtggag
     7801 aaaatgttag gaaaggggtt acgtttatat gtaattgctg tggatgttgc tgtgaagcaa
     7861 tggtagcagc taaaagattt ggaaatcttc atccagtaca gacaacaagt tttataccaa
     7921 atataaacca tgaaaattgt gtaaagtgtg gtaaatgtat aactgcatgt ccaatagatg
     7981 cgataagcaa ggttaaggaa gatggtaaag aatatataaa aattgatgag gatagatgtt
     8041 taggttgtgg agtttgtgtt agaaattgcc ataaaaatag cattatgtta ttaaagagag
     8101 atgaaaaaat aataactcca gcaaattcag tacatagagc agttctcatg gcaattgaaa
     8161 aagggcagtt acaaaattta atttttgata ataatgctct ggctagtcat agagctatgg
     8221 gagctatact gtcagctata ttaaagcttg aaccagctaa aaagatttta gccagtaaac
     8281 aattaaagtc agtatattta gataaattgt taagtatgaa tgataaatag aaactaactt
     8341 aaaaaaggca aagttttaga cttatctata tcttaataaa cttatcattt aatatttaaa
     8401 tttaattttt acacatataa aacttataac ttaaataaaa aaaggctata ttcaattaat
     8461 attacttgaa tacagccttt tttagttatg ttaagctatt tcttttattt gcttaaatga
     8521 gttttcattt ccatttccac caatttgtgt tacagattta aatctcatag ttttaaatag
     8581 ttctttttga ttataatcta gtgagtttcc tactagcatt acaggagatt tagtttttcc
     8641 agccaaaact ccaacagata atccgtctat taaatcatct tgtttattca ttccattttt
     8701 agcaacatat aaattgtcta tcttagaatc cttgtaaaat tcttttatta cttttgcatt
     8761 agtttcattt ctagtgttac ctgatattct ctgtggattt tgaagtttac tttctatatt
     8821 tttagaaact gtatattctc caccgattac ataagattta tcaatctttt tgtttttaaa
     8881 taagttattt atttcagtta tattactatt ttcattagta agtattatag gcatatcatt
     8941 ttgagctgat acagctccaa tgcttactgc atcggctaat cctttttcgc cattaactac
     9001 tgaaatcttt gatatattgc taatattgtt caattcttta gcaatgttca tagaagtttg
     9061 atatctatca cttcctgaaa ttctagatac agagatattg tttgatttta tagtatctaa
     9121 agatttttca tttatagaag cctctccacc aactacatat acatttttaa cttttaatct
     9181 ctttaattca gccaaagttt tttcatttat ttgagagctt ccagtcaata atataggagc
     9241 gtttttcttg tatgcaaatg gtgttgcggc caaagcatct gcaattgctg aatcgtttat
     9301 taaaactgca ttttcagcat tttcccaacc tgcttgactt atcttaatag aagtttcata
     9361 tctatcagtt cctgtaagtg cacttgcatt tacttcatct tgatatgtaa cctcaaatct
     9421 agcatttgga cttcctaaga aaacaattac gcctcctttt ggcaatctaa ctctattgtt
     9481 ctttgtaact aaagaatagt ttacaggaac acctttatca tcataaacag caaaagctga
     9541 attttgagga agattaactt ctattttttt atttgcgata tcgtcaccaa tcttatacca
     9601 ttttgcataa ccattggatt ctagttcaca agtaaaagat ttttctgctg gcaaattagt
     9661 tatagaatct tcactaacat acgttagtgt tccaaaagat aaatactctg taccattttc
     9721 tttatgaagt ttaatgtcgg ataaatcacg acctattaca ccaggtattt ctataaaagc
     9781 atttgagtta ttctcatcca ttattttagt gttaactata taacctggag tttcatcaga
     9841 aagactaaaa cttgctttta cagagccaaa catatagctt tgagaagtat atttttcatc
     9901 cactagatag taacctttgc cacttctttt tttccacact tctttgacac tatttgatat
     9961 attgttgtca tctacttttt gagctacata gtataaactt gctgtctgac ctaatccagg
    10021 gacatcatca taagagctca tatttaaata tgtgatatta tttttttctt ttacaaattt
    10081 taaacaactg ttaccctttt cagatacaaa tctatcttga cctatgtata cgtatttatc
    10141 ttcaggtcca ttttctatat aaggagaaga tactgttaaa gtaccattat cattaacatc
    10201 tacttttatc atatttgaag aagcataaag accactattt tcttttagag agctaggcat
    10261 tttaacttgt tgtggcttac taaatgtttt atcaggtttt atttctttaa ttttaccttt
    10321 ttcttttaat gcagatagta agatttcttg tccaattatt tcgttaagtt gactacttcc
    10381 accagaagaa agaactgcaa ctgccatatt ttcatctgga agaactataa gattactgtg
    10441 gaataataaa gagtctccac cttttgtaag agcttttaaa ttatactgat taaatggata
    10501 agtgttgaca caatcccacc ctaaaccata tccaagtata gaatcttctc cttcaggcca
    10561 tagaccattt aaatattctt tgttttccat agcttttact gaagcaggag aaagaatacc
    10621 atttgaattt ttcataaatg tttgtgcaaa tgtacataga ttttctgcac ttgaatataa
    10681 accacctgca cctatagcat taaggttatc ttgtggaact gcatcttccc aataaggtac
    10741 atatgctttt gctagttttg aactatcaaa actattttct gtagtttttg tattttgtaa
    10801 gttaagtgga ttatttatgt atttatcaag aaaattagta aatgacatac cagatactct
    10861 ttctactaaa atttcagcta aagtaaatcc atcattacaa taaacggaaa aagctcctgg
    10921 tttagctttt aatctttgtt tttgtaattc ttttaagaaa ttatcatgac cataagaatc
    10981 attgtctgct aataaaatag tgtttttaaa actacttccc atcaaccctg atgaatgatt
    11041 taataacatt cttggagtga tttccttgta tctatcatca gccattttaa attcaggtat
    11101 atactttaca acaggagtat ctaaatttaa ttttccatca tcaaccaact tcataacagc
    11161 tgttgtagta aacattttac tgatagatgc gatactatac atattatcct tatttaagtt
    11221 tttgttatcc tgtttgctgt aaactccacc attaccagaa atctcaattt tgccattatc
    11281 aatcaaagca tattgagcac ttacagcacc atactttgat agcaaagttt ggattttctc
    11341 tttagcaaga gctttagttt ttgtgtaaga ttccgcgttt ttactataat catgtaattc
    11401 tatatcagaa ctttgatatt tttcggaatt atcttttgta ttatcagcaa aagtaatatt
    11461 tggagcagca gtaaataaca tactcaatac taatgtacta gataataatt tagagatcct
    11521 ttgcttaaac atattacatt acctctttcc ctttttaata atttataatt agcataacat
    11581 tcttaattat tttaatcaac agattattat caataaaaaa agcttttata agtatagctt
    11641 tttatataac agagaaaaat ataaaaataa tatttgttgt tgta
//
LOCUS       DAEPOZ010000120        11559 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.120, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000120 DAEPOZ010000000
VERSION     DAEPOZ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11559
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.120"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            258..686
                     /locus_tag="KR440_002340"
     CDS             258..686
                     /locus_tag="KR440_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBH0699379.1"
                     /translation="MYGVIGATVLVIFVILTAGIMQGKKVDFQLPTVFMIVTFIVSAI
                     SEEIVFRGYIQTRLTGLIKNSVLSSCINAFLFLSTHYPVKWISSGDFSFAILSGFYVI
                     CLILLNFSCDLVYRKTNCLWGAWLLHILYNIGTGVLIFTT"
     gene            806..1414
                     /locus_tag="KR440_002341"
     CDS             806..1414
                     /locus_tag="KR440_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBH0699380.1"
                     /translation="MVDNLIKYIHKSNIAIVVLHEIYGVNNFIKDVCQKYYKGGYDVF
                     CPELLGEKRVFSYLKAQEAYDFFINNVKFDIYKKVEKLINGLKSEYKHVFVVGYSIGA
                     TIAWRCSENSLCDGIICCYGSRIRDYMDVIPTCPILLVFAKYDSFDVDYVSSQLSRRQ
                     NIQIEILDANHGFIDTYSKNYSNAQTEVFNIKEKNFLSKCLG"
     gene            1938..2150
                     /locus_tag="KR440_002342"
     CDS             1938..2150
                     /locus_tag="KR440_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBH0699381.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            2163..2384
                     /locus_tag="KR440_002343"
     CDS             2163..2384
                     /locus_tag="KR440_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBH0699382.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            2484..4640
                     /gene="feoB"
                     /locus_tag="KR440_002344"
     CDS             2484..4640
                     /gene="feoB"
                     /locus_tag="KR440_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBH0699383.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTIIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHVPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            4666..4818
                     /locus_tag="KR440_002345"
     CDS             4666..4818
                     /locus_tag="KR440_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBH0699384.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            4998..5708
                     /locus_tag="KR440_002346"
     CDS             4998..5708
                     /locus_tag="KR440_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBH0699385.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            5793..6563
                     /locus_tag="KR440_002347"
     CDS             5793..6563
                     /locus_tag="KR440_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0699386.1"
                     /translation="MFEKIHKRFKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVVFGVNKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            6556..7287
                     /locus_tag="KR440_002348"
     CDS             6556..7287
                     /locus_tag="KR440_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0699387.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNIYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            7321..8280
                     /locus_tag="KR440_002349"
     CDS             7321..8280
                     /locus_tag="KR440_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH0699388.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(8378..8974)
                     /locus_tag="KR440_002350"
     CDS             complement(8378..8974)
                     /locus_tag="KR440_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HBH0699389.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVNKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
     gene            9220..9819
                     /locus_tag="KR440_002351"
     CDS             9220..9819
                     /locus_tag="KR440_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699390.1"
                     /translation="MKLKRTLFVGLALVLAIGITACARDNEKPNENAGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKRMNDDLIAEGERLVKDIDERIEKLDKVSDEDMKKGQN
                     DFMKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
     gene            complement(9987..10454)
                     /locus_tag="KR440_002352"
     CDS             complement(9987..10454)
                     /locus_tag="KR440_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699391.1"
                     /translation="MENDTIKADDILNYCLSNLDDVVLMDSWGERAIYYNPNGVLKRG
                     VYVLTIKEKDSNHDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIEESYNFAKQKFKKRKNK"
     gene            10565..11476
                     /locus_tag="KR440_002353"
     CDS             10565..11476
                     /locus_tag="KR440_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBH0699392.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SVGFPLYSKVGAAGGYHVLKERILPPITFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENMRRKLVFWTPDRHCSTPLLKELFNIVMNEFTIKIEYSSK
                     QKNSIRTIVPIGLYAMNGLWYCPAYCIKSESVKEFRADRIVKILSIENLSNKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLIKK"
BASE COUNT         4341 a         1249 c         2050 g         3919 t
ORIGIN      
        1 ataaagaata tgttttatta tgtaagaatt attctaaaag tgatggagta aaagcaatat
       61 ttatttattt gatatctatg ttactattgt ttttccaagg ttatttgtat acaacaaatt
      121 taagttcaac aattttaact aactcacaaa tgatattgtc aatcttgatt ttacttatag
      181 ggctgggatt tgtactactt tcgaaggaaa aactaaatac gattgggatt acaatgtgaa
      241 atttaaataa aagtcttttg tatggagtta ttggtgctac tgtactcgta attttcgtta
      301 ttttaacagc tggaattatg caaggaaaaa aagttgattt tcaattacct actgttttta
      361 tgattgttac atttattgtt tctgctattt cagaagaaat tgtttttaga gggtatattc
      421 aaacaaggtt aactggatta ataaaaaata gtgtactttc atcttgtatt aatgcttttc
      481 tttttctatc tacacattac cctgtaaaat ggatttcaag tggagatttt tcatttgcaa
      541 ttctatctgg attttatgtg atatgcctta tattacttaa cttttcatgt gatttagtat
      601 atcgaaaaac taactgtctg tggggggcat ggctactgca tatcttgtat aacattggca
      661 caggagtgct gatttttact acttagtcaa ttttactttg cagaaagttt atcgtaagaa
      721 acggattatt ttataagtca aattttagag ctagtaaatt gacatactta aaataattta
      781 aactagatgt aaaaaatatc gacaggtggt ggacaatctg attaagtata tacataaaag
      841 taatatagct attgttgtac tgcatgaaat ttatggagtt aataatttta taaaagatgt
      901 ttgtcaaaag tattataaag gtgggtatga tgtgttttgt ccagaactct taggagaaaa
      961 aagagttttt tcatacttaa aagctcaaga agcatatgat ttttttataa ataatgttaa
     1021 gtttgatatt tataaaaaag tagaaaaact tataaatggt ttaaaatcgg agtataaaca
     1081 tgtatttgta gttgggtata gtataggtgc aactattgca tggagatgta gtgaaaactc
     1141 tttgtgtgat ggtataattt gttgttatgg ctcccgaatt agagattata tggatgtaat
     1201 tccaacatgt cctattttgc tagtgtttgc aaaatacgat tcattcgatg ttgattatgt
     1261 ttcttctcaa ctttctagaa ggcaaaatat tcaaattgaa attttagacg caaatcatgg
     1321 ttttattgat acatactcaa aaaactattc taatgctcaa acagaagttt ttaatattaa
     1381 agaaaaaaac tttctatcta aatgtttagg ctaatatgtt ttattataaa atatagaaaa
     1441 tttaatcaat ttgaaaaaca taatgacact actaatgtta aaaaatatta gtagtgtcat
     1501 tatatttttt gagatagaga tagtcagtag aatcatagaa ttaattatgt ttttatgatt
     1561 gtattagatt aatatttaaa ttacattgaa aagaaaaaaa tcattactat agaataatat
     1621 ctttatcaaa agaacattct tatgcagaat atggttatga gtatctaaga gaaatctcaa
     1681 aatatatagg atagcagtat gaatttttat agattttatt aatacaataa atcaaatgag
     1741 aaaaaaaatc aaaagaatac aataatcact tgcaaaaaat atgctattgt gatagactta
     1801 aatcaaaatt aggaattgta agaataagga aaaagataag taattatttt aacgagtcaa
     1861 aatttaagaa attgtgattt atatataaga tagaggttgt ttgtaaattt aaattggttg
     1921 aagaggagga atattatatg atgccattga tattatcaaa tacaggtgaa gaagtagtta
     1981 taaagaaaat ttcgggaagt gataagacga ggactttttt aaatagtata ggttttgtag
     2041 taggtacaaa tgttaaaata gtttctaaga tagatggtaa cttaataata gacgtaaaag
     2101 atactagagt tgcattagat aaaaaaatgg caagtagaat aataatatag ggaggtaatg
     2161 acatgaacag actgaaggat attaagtgtg gagagactgt aaaagtcaaa aagttagaag
     2221 gagaaggagc tacacgaaga cgtataatgg atatggggat tacaagaggt gttaatatat
     2281 ttataagaaa agtagctcct cttggtgatc caattgaggt aacagtcaga gattatgaac
     2341 tatcaattcg taagtcagat gcagaaaaaa ttattgtgga ataataaaga ttaattagag
     2401 tagggttaat gtaattatat aatgagaatg attttctgta tcacaaacag tgatgtattt
     2461 aaataataat ggagggaatg gaaatgtcaa taaaaatagg tctcatagga aatcctaact
     2521 gtggtaagac aacaatgttc aatgggctta caggctcgtc gcaatatgtt ggtaactggc
     2581 caggagtaac agttgaaaaa aaaggtggaa aattaaaagg aaataaagat gtggaaatag
     2641 tagatttacc aggtatttat tctttatctc catatacatt agaagaagta gttacacgta
     2701 attttatgct agatgataag ccggatgcag taataaatat tgtagacgca tcaaatatag
     2761 agagaaattt atatcttaca actcaagttt tagagctagg tataccgacc attatagctc
     2821 ttaatatgat ggatattgta aataagaatg gtgataagat aaatattaaa gaactatctg
     2881 aagttatagg ttgcccagtg gtagaagtaa cagcagttaa aggtcaagga attatggagg
     2941 ctgctgaaaa ggcagttgaa cttgcaagta gtaacaataa attaaatttc aaattacctt
     3001 ttgttgatga aagtaaggac gcaatagaaa aaattgaaaa aataatagaa gaaaagactc
     3061 catatataga tgtagaaaca cgctggttag ctataaagct atttgagaga gatgaaaatg
     3121 ttatacaaaa acttgatatt tctaaaacaa tattgaatag tattgaagaa ataactagaa
     3181 attgtgaaga tgaattggat gatgatagtg aaagtataat aacagccaat cgttatgaat
     3241 ttattagttc aataatttca agtataataa agaagaatcg aaaaggtaag gaaactgtat
     3301 cagataagat agataagatt gtaacaaatc gtatactagc attacctatt tttgcactta
     3361 taatgtgggg agtttactat atagcagtaa gttcattggg tactatagca actgattgga
     3421 caaatgatgt actgtttgga gaaattattc aaggcaatgt aagtaatttt ctagcttctt
     3481 taaatgtagc agagtggtta caaggacttg tagttgatgg tctaattggt ggagttggag
     3541 cagtactagg atttgtacca caaatcatgc ttttattctt attattgtca atattagaag
     3601 attgtgggta tatgtcacgt gtagctttta taatggatag aattttccgt aagtttggtc
     3661 tttcaggaaa atcatttata ccaatgctta taagttcagg ttgtggagtt ccaggagtta
     3721 tgtcaactcg tactatagag aatgatagag atagaaaaat gactattatg ttaactacat
     3781 tcattccatg tggagctaag attccaataa tagcattatt tgcaggggca ttatttggag
     3841 gtgcttcatg ggtagctcca tcaatgtatt tcttaggtat tgcaatgata atcatttgtg
     3901 gaattatatt aaagaaaact agtttatttg caggagaacc atctccattt gttatggaat
     3961 tacctcaata tcatgttcca agtgcaaaag gtgttttaat acatatgtgg gatagaggaa
     4021 aagcatttat aatcaaagca ggaacaatta tatttgttgc ttgtggagta atctggttct
     4081 tacaatcatt taattggtca ttacaaatgg tagatgctgg agatagtata ttagctagct
     4141 taggaaatat agttgcacca atatttgctc ctcttggatt tggaaattgg caatcatcag
     4201 tagcaacagt tacaggtcta gtagctaagg aaaatgtagt tggaacattt ggagttttat
     4261 ttggtatatc agatgcaaca gagcaagacc caacacttct tgcttcagtt gcaagtatgt
     4321 ttacagtagc tagtgcgttt gcatttatgg catttaatat gctttgtgca ccttgttttg
     4381 cagctattgg agccataaaa agagaaatgg gttcatggaa atggacatgg attacattag
     4441 gattccaaac tttaacagcg tatataattg ctcttcttat aaatcaagtt ggaagtttag
     4501 ttttaggtac tggaggaagt attgctgggg caataatatc tatatttata gcagtagcag
     4561 tagtctttgt agttttaact tattcaaata aaaatatgaa aaaagaaaaa atgggtaaat
     4621 taagttatat gaaaaattaa ttcatataaa taaggagtga tgaatatggc tacatttatt
     4681 atagcagcta ttgtagtagt attaatggca ctagctgtaa tgtatatggt taaaaattca
     4741 aaaaagaatg gtagcagttg tggatgtggt tgttctggat gctcaagtag taagagttgt
     4801 catagcgcta agagataaat aggataagag cctgataaaa agttaaattg ctttattatc
     4861 aggttcttat tttttatata aataacaaat ttcatatttg aagtaggtta gactatgctg
     4921 taattttact gtataattat atataaatat atgattagga gggattattg ctaaattaag
     4981 caatataagt gaatattatg tttaaatttg atgttacaaa cgagcttatg tgtatagcaa
     5041 ggggaagtgg taaatttttt gcaaaaaaag gtgctatggt agcctttaaa ggaaatttta
     5101 attttgaaaa attgctgtta ggtccaagta atggcggtgg attgggaaga gcattattag
     5161 gtcatgttca gaggtcattg acaggagaac aaatgccaat aatggtggtt gaaggtgaag
     5221 gtgaaattta cctagctcaa aatgcttacc atgtagatgt aatatcaatt gacccaggtg
     5281 attccattta tgttgagagt gaaaacttat tggcattttc tgaacaatta aattacagtg
     5341 taaaattgat tggttcaggg gtaatatctc aaaaaggtct ttttactaca catttagtaa
     5401 ataaaacagg tcaaaatcaa gatgttgcta taataacaga tggtaatcct ctaattcttg
     5461 aaggaccttg ttgtgttgac ccagatgcat tagtagcttg gactggaaga gaaccatatc
     5521 ctaaagtatc aaaactatca tggaagactt ttattggtca gacttcagga gaatcttatc
     5581 atatggaatt tgttgaacct ggacaaatag tcattataca accatctgaa agattgtctg
     5641 gcttaaaaac agattctaca ccttctgata gaatgagttc aggtggatta agacttggca
     5701 tagattaaat cttatgatat agcttaatta ttatttaaga tattaaaaaa taaaaagggt
     5761 agcagggata gatatgttaa aaggagtttt atatgtttga gaaaattcat aaaaggttta
     5821 aaggatatta tattttttta ataattttaa ttttatggaa gatagtatgt gttttgggta
     5881 tatggagtag ctatattctg cctcctcctg aaatagtctt agatactttt ataaaaatga
     5941 tatatgacgg ttctattttc ttaaatgttt atgccagtgt aagaaggata ttaataggtt
     6001 ttttaataag ttcattgatg gctataccac ttggagttgt atttggagta aataaaaaaa
     6061 tgtatgaata tttcaaacca ctatttgaat ttatgcgaag tacacctcca ctagctttgg
     6121 tgccaatgct tattttatgg tttggtatag gtgaagaatc taaaattata attattattt
     6181 tggcatcctt ttttccaata tttttaaata cattgaaagg tataaaaaat tgtgacaata
     6241 aattaataga agtaggtaaa tcttttaatt tatctaaaaa gagaattttt tataaaataa
     6301 taatccctaa ttctatgctg gatattgttg ttggacttag tttagggctt gggtatagtt
     6361 ttagggctat aattggagca gagcttattg cagcttcttc aggactagga tatttgattt
     6421 ctgatggaaa agatatgtct agaactgatg ttgtattggt aggaattttt gttattggat
     6481 ttttaggtat actttcagat tatctatttt caaagataat aacaaaattt agcaaaggga
     6541 agcaggttga tttaaatggt tagaagtgga tttttgctag agaatatata taagaaatat
     6601 ttagttgata ataaagagca tttagttctt gataatatat ctttaaatat atctagtgaa
     6661 gagataactg ttatattagg tgaaagtgga tgtgggaaaa caacgctact tagaatttta
     6721 gcagggcttg aaaatgctac tagtggaaac atatattttt ttaataatga taaaaagtgt
     6781 actccaaaag taggaatggt atttcaagaa agtagactta tgccatggct gaatgtaagt
     6841 gaaaatatac ttttacatac tgaaaaagat aatagaaata aagttgattt agacaagtat
     6901 ttaaaaatga tgaagcttga aaagtttaaa aactcatatc caaatgaatt atcaggagga
     6961 atggctcaca gagtaagtat agcaagagca ctttccttca atcccgatat tttgctgatg
     7021 gacgaaccat ttgcagcgct agattacttc acaagaagaa aaatgcaaaa agaagttgta
     7081 aatatccata aaaatactaa aaaaggtgta gtatttgtaa ctcacaatat tgaagaagct
     7141 atggaaatag caaagaaaat aattgttttt agtaaaaata aaaggattaa acaatttagt
     7201 gtcgaagatg aatataatag agatttaact aaaaactatt atataaactt gaaaaaagaa
     7261 atattaaggg aattggggga gttttaattg attaaaagaa acaaaaagat tttagcagta
     7321 atgacatctt taataatggt gttggttggg actgtaggat gttcaacaac aaatgataag
     7381 aaagatgaac ctgcaaaaaa agaggctagt ttacctaagg aaataaattt aacttatgta
     7441 aaatcaccac ttaatgttcc tagtatatta gaaaaaaagg atgatttatt tggaaaggaa
     7501 tttaaaaagg acaatataaa agtcaacttc catgaattga caacaggacc agagcaaact
     7561 caagcattag cagcaggtga gctagacttt ttacatgcat taggaggaac gtctgcaata
     7621 atagctgcat caaatggtgt tggtcttaaa attacgaata tttatagccg ttcaccaaaa
     7681 ggatatatgc ttataactaa taatgataat attaaatcta taaaagactt aaaaggtaaa
     7741 aaaattgtag gtccaaaagg gactatactt catcaactgt tgattgctgc acttgctaaa
     7801 gaaggctata ctatagatga tgttgaatat gtaaatatgg atattcctag tgctgcttct
     7861 gcattagaaa gtaaaagtgc tgatgtagca atgcttgcag gtccagtggc attgaaaacg
     7921 ataaattcag gtgccaaaat gattgtaaat ggagaaggat tagtaagtgg aataatagtt
     7981 actgctgtaa gtgatgactt tgctgaaaaa tatcctgaat tagtaaaaag atttatgaag
     8041 gttcatgaag aaactttaaa atatatgaat gaaaacaagg atgaagtgat ggatgttgta
     8101 tctaaagaag ttggtttatc tttagatgaa acaaaagaaa tgtattcttg gtatgacttt
     8161 agttctaaaa taacggacaa ggatataaaa gaattagaag atacacagga atttttgatg
     8221 tctaatggaa tgcaacaaaa gaaaataaat attaaagata tgttgtacaa tcaaaattag
     8281 aatactttaa aatattaaag tggaaaatac acccttattt attatggaaa ctgtatggta
     8341 aaagagggtg tatttttatt attatacaaa aatacaatta aaaacaactt actattttat
     8401 ctacacttat gtagtctagt aattttctca ttttcttacg gtgaagtaaa aactttgaat
     8461 atgtgtcctc ggattgtttt gtgtcaccat aaactttcca atatttaatc atagaaactt
     8521 cataattact atatcccata aatccaatca catctttaag aggatatcct aaaaaaatac
     8581 ctatttcatc aggaaaaaca tcactcttaa gtttatttac taaatgctct aaataagcat
     8641 taacattcgt attttgtttg tatcctaaaa actttaaaaa gtttactgtt tttttacatt
     8701 ttaatttttc tgataatgct tttttattta caaataagat ttttaatccc tttttttctt
     8761 tatcaataac tataaaatct attttcttac atttatatag atatttataa atatcattaa
     8821 ttttttcctc tctatttaca tcagtaaacg aaatatttat tatttcagca ggtttagagc
     8881 ctaaaaagac tggacctaaa acctctaaaa gccattttat atatgatgat tcaattttat
     8941 ttttacaaca aaattgtgaa ttacaacaag acataaaaga taacctcctt caaatgaaaa
     9001 ttaatatcaa gtaattagat tatactatga atgataatca atatcaataa taaaattata
     9061 atttttttat ttaattattt attcattatg atttccaaaa aaataaatta taacttgttt
     9121 aattttagta aataaaaaat aaaaatatag aaatactaaa ttaagaaaga aaattattaa
     9181 aattttttgt agtacaaaac atctaggagg gtaataaaaa tgaaattaaa aagaacttta
     9241 tttgtaggat tagcattagt attagctata ggaataactg catgtgctag ggataatgaa
     9301 aaaccaaatg aaaatgcagg aaaaaatcct aatgaatctg taaataataa tgaaggatat
     9361 atgaattatt attcaacaag ttataatgat tatatagcag gtctaaatag atattctatt
     9421 tatgatactc cagaaagtat aaacaatcag tttaaagata aagaatatcc aggaaatgaa
     9481 aaatatctaa gtgatgtaaa ggctgcatat aaagatagta gagataaaat acaatcattt
     9541 gtaacatctc ttaaaaaaga cggtaaaaca gaagatacag agttaaagag aatgaatgat
     9601 gacctaatag ctgaagggga aagattagta aaagatatag atgagagaat agaaaaatta
     9661 gataaggtat ctgatgaaga tatgaaaaaa ggtcaaaatg attttatgaa actagttcat
     9721 ggaactgaag atgttggaaa cgatattggt agtggtttta gaaaaatgat aaaagatatg
     9781 aatgatagat tgggaataac aggaaataat aaaaaataat agagtaaata gttttaaagt
     9841 tagaaataaa tatttgaaat atagcgaagc cttgtaatta atatattttt tggcttcgct
     9901 tttttgtatg ttatcaagat actaattttt atcttgtaac ctcatatttt cattatagtt
     9961 tatttagagg ttattttttt atgtttttac ttgttttttc tctttttaaa tttttgtttt
    10021 gcaaaattgt aagattcttc tattaaagtt ttaaagttct caaatgtctt ttcagtaggg
    10081 cttaggacgc atatccatcc catccatgag tagataggat gtggcatgat tgtgtccaat
    10141 tttgtaaaat caaaatccat atcaactatt tgccctacac ctggacgctt tggaatatat
    10201 ccaaacattt caataaaagt ttcttttttt aatcctatat tcacacggta tacatttgga
    10261 cgactaacca acgaaccttt atcatgatta ctgtcttttt ctttaatggt aagaacatat
    10321 acccctcgct ttaaaacacc attaggattg tagtaaattg ctcgttcccc ccaactatcc
    10381 attagtacaa catcatctaa gtttgataga caataattga gaatatcatc agccttaata
    10441 gtatcatttt ccataaaata tcaatccctt ctttctttaa tattattatt gctttagatg
    10501 caagaatatc ttatcatata atggtgacaa ttattgtcac tatatttaaa ttaggagtat
    10561 gaatatgtca agagctgagc gtttaattga actgatgata acaataaatg caaaaagaag
    10621 ttttacagcg ggagagctag cagaagaatt ttctgtatca aaacgtacta tactaagaga
    10681 tttacaggta ttggaaagtg ttggatttcc actatattca aaagttggag cggctggagg
    10741 atatcatgta cttaaagaac gtatacttcc accaattaca ttttcagaga gtgaggtaaa
    10801 atctatattc tttgcatatc agtcactaga gtattataat gatttaccat ttgagcaaga
    10861 aactatttct gttttaaaga aatttttaaa ttgtatacca aatgatattc aatataatat
    10921 tgagaatatg agacgtaaac ttgttttttg gacaccagat aggcattgct caacaccatt
    10981 attgaaggaa ttgtttaata tagtaatgaa tgaatttaca attaaaattg aatattcatc
    11041 aaagcaaaaa aatagtatac gtaccattgt tcctattggt ttgtatgcta tgaatggact
    11101 ttggtattgt ccagcttact gtataaaatc agaatctgtt aaggaatttc gtgcagacag
    11161 aattgtgaag attttgagta tagaaaactt atcaaataag aaatataagg ttttatcttc
    11221 tatacatgat tatcttaaga atatggaagt aggtacagat tatcatatta aaattaatct
    11281 gacagatgag ggagtaaaac gttgtgaaac agaatttttg ttggcaagag gattgaaaat
    11341 tttgtctaag ggtggataca tagatatgta tataccaaaa tctacactta attgggtggc
    11401 tgaatatttt ttaacttttg gaaaaaatgc tacaataata gaacctatag aacttaaaca
    11461 tttgattaaa aaatagctac gcacacttgt gactttagtc atgagttagt agctaaatta
    11521 gtcagcatat agggaaactt atatgtagag ataggataa
//
LOCUS       DAEPOZ010000121        11510 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.121, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000121 DAEPOZ010000000
VERSION     DAEPOZ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11510
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.121"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(85..1980)
                     /locus_tag="KR440_002354"
     CDS             complement(85..1980)
                     /locus_tag="KR440_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HBH0699393.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKSETISVSSKNHKLVRDLHKVLTDVKDGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
     gene            complement(2606..2749)
                     /gene="scfA"
                     /locus_tag="KR440_002355"
     CDS             complement(2606..2749)
                     /gene="scfA"
                     /locus_tag="KR440_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HBH0699394.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            complement(2815..3177)
                     /locus_tag="KR440_002356"
     CDS             complement(2815..3177)
                     /locus_tag="KR440_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HBH0699395.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEVGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            complement(3235..3528)
                     /gene="yajC"
                     /locus_tag="KR440_002357"
     CDS             complement(3235..3528)
                     /gene="yajC"
                     /locus_tag="KR440_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBH0699396.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            complement(3662..4783)
                     /gene="tgt"
                     /locus_tag="KR440_002358"
     CDS             complement(3662..4783)
                     /gene="tgt"
                     /locus_tag="KR440_002358"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBH0699397.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITSIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPQDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            complement(4862..5347)
                     /locus_tag="KR440_002359"
     CDS             complement(4862..5347)
                     /locus_tag="KR440_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699398.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            complement(5359..6384)
                     /gene="queA"
                     /locus_tag="KR440_002360"
     CDS             complement(5359..6384)
                     /gene="queA"
                     /locus_tag="KR440_002360"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBH0699399.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFLYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGNNVICVGTTSCRTIESACNEDGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            complement(6702..7721)
                     /gene="ruvB"
                     /locus_tag="KR440_002361"
     CDS             complement(6702..7721)
                     /gene="ruvB"
                     /locus_tag="KR440_002361"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBH0699400.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            complement(7828..8430)
                     /gene="ruvA"
                     /locus_tag="KR440_002362"
     CDS             complement(7828..8430)
                     /gene="ruvA"
                     /locus_tag="KR440_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBH0699401.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASSAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            complement(8423..8932)
                     /gene="ruvC"
                     /locus_tag="KR440_002363"
     CDS             complement(8423..8932)
                     /gene="ruvC"
                     /locus_tag="KR440_002363"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBH0699402.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKVIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            complement(9244..9873)
                     /locus_tag="KR440_002364"
     CDS             complement(9244..9873)
                     /locus_tag="KR440_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699403.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKNNGVQTSK
                     VVSASANMDEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMNATIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            10076..10399
                     /locus_tag="KR440_002365"
     CDS             10076..10399
                     /locus_tag="KR440_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBH0699404.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            10667..>11510
                     /locus_tag="KR440_002366"
     CDS             10667..>11510
                     /locus_tag="KR440_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699405.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIK"
BASE COUNT         3600 a         2039 c         1279 g         4592 t
ORIGIN      
        1 tatctacact atgtcttaca tattaacgca ttgtataatc atatattatc taaataaact
       61 tttaatatta attcctatat cttattagtt cttagtaaat aagtttattt tttctgctat
      121 tttttcaata gcgtttttag ctattccttc acctatttgt actaacttag tagcattctt
      181 aagtcttaac tcaactactt cttcttgtga tttagaaagt tcttctgtag ttaatactat
      241 tggagccttt gaactagctg caagtggtgc tgctgtaagt gcatcaacta attgtccatt
      301 tccacctaca taaccatctt ttgctacata tgctttagtt gcacctgcat agtatttatt
      361 gattacttta gcattagtat cttttctatt ttctccagct aatctttcaa ctttattatt
      421 taaatctata tcaactaatt tgtcttcaac ttcttttgat acagaatttt ctccacccac
      481 tatttctaat tcttttcctt ttaataattt ttcagcattt gcatctaatt cgttacctgt
      541 aactattatt ggagcaccaa attgagcagc tctagctgat atactcatag catcagcttc
      601 tccatttcca cctactacaa aagcagtctt aaatgtatct tttaattgtt ttgctatttc
      661 taaagaagtt tcaaatctat cttctccaga aactctctca actttactta cacctaattt
      721 agaaagtttt tcttcagttt cttttgatac tttagattct ccacctacta tatatatttt
      781 cttagaagat agattacttc caagtactct taatatttca gcttctattt ctgatggtag
      841 tgaatctttt ttagataata atattggtgc atttttttga gaggcaagtg gtgctgatgc
      901 taatccatca actatagcat cttctccaac taaaattata gcttctgctt ctccatcttt
      961 gaatctctct ttactaactt ctactgctgt tgtaaatctt gaatcaccag ataaaacctc
     1021 tacttttaat tcttttccat cttttacatc agttaatact ttgtgtaagt ctctaactaa
     1081 cttatggttt ttacttgaaa cacttattgt ttctgacttt tcatttgcct tgcttaaaac
     1141 tatatcaaag cttgattttt ctggtttatc tttatcatca ctatcttctt gttcttgagt
     1201 aaggattgct tcattttcaa atgttaaagc atatccattt ttaactttat taactatttc
     1261 tcttcctaat gtagttaatc taattccatc aaataattct tctgctgata tgtcttgtaa
     1321 atctgcgttt ttaactgtta tcgtatgtaa ttcttttgaa tctatgtcac tataactctt
     1381 ttcataacct tcaaaagtac cttcttctga tgttatcact tttgagaaat ctaaatgttg
     1441 gtctcctaca tttaatgtaa taacatttgc tgaatctttt gctcttttta cttcaactgt
     1501 atttgtagat tttatagttg ctgttaactt gttatcagaa tcttctgcta aagttgcatt
     1561 gtaagcatta actgcatcta ctatttcttg agcagttttg tagttttcaa ttttatagtc
     1621 aactactttt ccacctaaca cttgatgtcc tttatcttga atagtaactt ttaaactttg
     1681 cccttctgtt aaagagttta ttttattttg taattgagtt gcattagtta agtttacatt
     1741 gtctactttt atatcgtaaa ctctatcatt tacatttgct cctgcttttg tatcttcaaa
     1801 cttaacttct aatgcttttc taacctcttc gattagttta gcagaatctt tcgcacttac
     1861 tgtgtaattt ttatcaacat tttctgtcac agtattgtct gcgaatattg gtgctgctgc
     1921 actcataact gtagcacctg ccattactat tgccatactt ctctttttgt tgcttaacat
     1981 atttatgtcc tccgaatatt attttttatt ttttatattc gtatttccca cacataaata
     2041 atattatata taatattatt tttcaatata ttttatgtgt ttacataatt tactaacaca
     2101 aacactaata atatcttctt gaaccccttc cccctactta gctctctata attatgctca
     2161 aagatagaaa tccctatttt atagtgattt tctactaaac aatcatatac taacaaaagt
     2221 attcaaactc catttttata tactactatg cactaatttt tgctttgctc ttattactta
     2281 aatatatatc actaaattaa tatctacctc catatattaa aatatataac atattataaa
     2341 atcatcataa attagagttt aaaattaaaa attattctaa tacatgtaat tgataaaaaa
     2401 taaaatataa gagtagctac tttaaaataa atgctcttat atgattagat atttttgcat
     2461 aattaaacga taagaaatca aatttaattt tacataagta gaatatatat ttttatataa
     2521 taattgaaaa taaaagagga tacctcataa tgataaaaga atcactttga gataccctct
     2581 tacaaacaac taaatcaata attttctatc tttcacactc ttgatttgca actgtacaag
     2641 aagttttaca agctgattga caagaagttt gacactctcc acatccacct tttgcagcac
     2701 tttgctttaa agtagcttga gataaagttt ttacatgttt attttccatt ttcaacgcct
     2761 ccatttctaa ttgtttccta ttgattatat cataaaacta ctttaatata aactctattt
     2821 aaagttgaca cccaatacgc ctccaatgcc tcctgctgct gatattagca ataacttata
     2881 aatcatccct acttcaaata caaacttttc ttgtgctaaa aacacagtca aaaaaataca
     2941 tactatgtac aaaagtccta ctaaaagtcc atacataagt cctttttctt tcatgtgttt
     3001 tgaagtgtaa aaccctccta tagctgcaga aatagtagtt ataaatgatg agaccatagt
     3061 tatattatct ccaccaatat ctgtaaatgt caaaaataag ttatacacta ataaaacagc
     3121 caaagttatt atatatgcat atcctagacc tttaaaaata taattagatt ttcccataaa
     3181 aaaacctcct ctcaaattaa tatatattcg agaaaaggct tttaaattac taatttagtc
     3241 ttcttctatt tcttcttttt tagatttaac agttcctata gcccattttt caaatggaac
     3301 tttagttcta tttggtccta attctaatat tacaacatca tcttcaactt tagctacatt
     3361 agctactatt ccacctattg taatgacctt gtctcctaca cttaggctac ttctcatttc
     3421 ttttaattgt ttgtcttttt tcttttgagg tctaatcatt aggaaataaa agattgcgat
     3481 tactactacc catagaccta tactcataat tatttgttgc tgtggcatta atttttcact
     3541 cctttgtaaa ttatactctt ttgtaaatca taataaatgt ttatataaca atttactttc
     3601 tctatctttg agtactattg aatattatat attaaaatga ttataattaa tagtgcaaaa
     3661 cctatatttc atatccatat tttgcaaaaa attcatttct aaaatcaagt aatctatctt
     3721 ccattatcgc ttgtcttatt tgtttcatta aatttaataa aaagtgaaga ttatgagtag
     3781 ttattaatct tgcacccagt atttcatttg cttttataag gtgtcttata taagctcttg
     3841 aatagttttt acaagcataa caatcacatt cagggtcaag tggagtaaag tcttcagcat
     3901 aagttgcatt tctaactact actttacctt ggctagtcat tgcagttcca tttctagcga
     3961 tacgagtagg taggacacaa tcaaacatgt ctactcctct tataacacct tccactaagt
     4021 catctggact accaactccc attaggtatc ttggtttatc ctgtggcata agtggagttg
     4081 tatgctctag cacgtcatac ataagtggtt ttggttctcc aacactaagc ccaccaatag
     4141 catatcctgg taaatcaata ctagttattt cttttgctga ttgttctctt aagtctttat
     4201 acatcccacc ttgtattata ccaaataaag cttgtttgtc ggtattatta tgggcatctt
     4261 tacatctttt taaccatctt gttgttcttt ctaaagaatt ttttacatat tctctatctg
     4321 atgggtatgg tgcacactca tcaaacgcca tcattatgtc tgaacccaat gcattttgta
     4381 tttccatagc tttttctgga gttagaaaat gttttgaacc atctaagtgt gacctaaatt
     4441 ctacaccttc ttcttttatc tttcttaaag gccctaagct aaatacttgg aaccctccac
     4501 tatctgtaag tattggttta tcccagttca taaacttatg aagaccacca gctcttttta
     4561 taagttcatg acctggtctc atatataaat ggtatgtgtt actaagtatt atttgtgaac
     4621 caatctcttt taattcttct ggtgtcatag attttacagt tgcttgagtt ccaactggca
     4681 tgaatattgg tgtttcaata atcccatgtg gagtgtgtaa tcttcctaac cttgcaccac
     4741 tctgcttaca agtctttatc aattcatatc ttactgcgta cataaagttc tccttaacca
     4801 tatttatttc tcattatttc ttaagatata tttgtaataa gctattcata taatttattg
     4861 tctaaataga tttatcttcg tcttctttgt tctgttgctc cttaattgct tcttcatcat
     4921 gaatactcat tatattaaga gcactcttgt cttttgttac taaaaacttt tttatagcaa
     4981 atagtcctgc tatagctata actcctatta aaacatctat cataccttca tttttaggca
     5041 gtagcaacat ttttctagct attgcgtaaa gtaatgcctc aataaatgct cctggtatat
     5101 gaagagatag cattactaca agttcaacac ctattactaa aagtaatatt tgtgctaaaa
     5161 aatcattaag ctgactgtat tgtactggat tttgaaaatc aactatatat gctccccaca
     5221 taagtcttag aacatctata gttcctaaaa atactgctat tagaactact actgctaaaa
     5281 cacgttcaaa tataaaagca atctttaata tagggttatt ctttttttgt ttttgtaaac
     5341 tatgcattca ttacctccct actttataat catagcatct ccaaaactga agaatctata
     5401 tctttctttt actgcctcat tatatgcatt taaaacatta tcctttccac aaatagcact
     5461 tactaacatt attagtgtag attctggtaa atgaaaatta gtaataagtt tatctaaaac
     5521 tttaaattta taacctggat atataaatat ttctgtccat ccactagttt ctttcatctt
     5581 tccatcctca ttacaagctg attctatagt tctacaacta gtagtaccaa cacatattac
     5641 attgtttcca ttttccttcg ctctatttat tttgtcagct gcttcttggc taaccatata
     5701 atattctgaa tgcatcttgt gatttaaaac atcttctact tttactggtc taaaagtgcc
     5761 taaaccaacg tgtagagtta caaaagctat atctacacct ttttctttta ttttatttaa
     5821 caattcttct gtaaaatgta atcctgctgt aggtgctgct gctgaaccat tatgctttga
     5881 ataaacagtc tggtatcttt ctttttcatc tagtctagca gtgatatatg gaggtagtgg
     5941 catatttcca agttcatcca atatttcttc aaaaattcca tcataaagga attttataat
     6001 tcttgaacct tcatctgata aatctacaac ttctccaatt agctttccat ttccaaaaga
     6061 aaatttagtc cctacttttg ctctttttcc tggttttact aaagcctgcc aagtatcttc
     6121 ctctgttctt tttaataata aaaactctat tttacctcca gtttcaagct tctctcctat
     6181 taatctagct ggtataactc tagtattatt taatactaaa caatctcctg gatttaaata
     6241 ttctattata tctctaaata ctttatgttc aatattacct gtctctttat ctaataccat
     6301 caatcttgag ctagctctat cttctatagg aacttgagct ataagttcct gtggtaagtc
     6361 aaatttaaaa tcacttgttt tcaatgttaa acttcctctc ttaaaatttc tccagctaca
     6421 ataatatcaa aaaaacaact tattttccac aatattacca taatctatca agaaattgct
     6481 atttttaaca gacaccatat ccacaatata tagatatttt tccacgatat acatgtctta
     6541 tccacaatat atagatacaa tacacacaat ttttatcgtg tttatccaca gtatctacag
     6601 atataattac cattttgtgg atatatttga atttttagcc attttagaaa tattttaatt
     6661 taatgactct tttattttat aaatactcgt ataatctatt tttatttttc attaggatat
     6721 ggaattttta agtgttcata tgctaatggc atggctactc ttcctctagg acctctattt
     6781 ataaatccta attgaagcaa atatggctca tatacatctt ctattgtatt tctatcttct
     6841 ccaatagaag ctgccaatgt atctaaaccc actggtcctc ctctaaattt ttctattata
     6901 gtcataagaa gcttctcatc tacaaaatcg agacccaaac tatcgactcc taaaagttcc
     6961 agagcatctt ttgccacctt atcagttatt tttccatcag ctctgacttg tgcaaaatct
     7021 ctaactcttt ttagtaatct atttgctatc cttggagtcc ctcttgaacg ttttgcaagt
     7081 tctaatgctc cattagactg tatctctgca tctaagatgc ttgaagacct aagtactatt
     7141 tttgaaagtt catcaactgt ataataatcc aatttacaaa taacaccaaa tctatctcta
     7201 agtggatttg taagcatacc agctcttgta gtagctccaa taagtgtaaa tttaggaagg
     7261 tcaagtctta tgcttctagc agatggccct ttccctatta ttatatctag gcaaaaatcc
     7321 tccattgctg gatataaaac ttcttcaaca cttctattga ttctatgaat ctcatctata
     7381 aataaaacat cattttcatt taaattcgta agtatggctg ccaaatctcc agctctttct
     7441 attgcgggtc ctgacgtaat tcttaaattt acccccatct catttgctat tatacttgct
     7501 agtgtagttt ttccaagacc tggtggtcca tataaaagca catggtctaa ctgctcattt
     7561 ctcgatttag ctgcttctat aaatatacta agttgttctt tggatttttc ttgacccaaa
     7621 taatcttcta aagtttttgg tcttaaactg ttttctatat ctatatcttc catcttcatt
     7681 gtagaagtta ttatcctatt ttcatcttca aaaccttgca tttatacccc tcctctcgta
     7741 cttttgaaaa tctactttat atattttcaa atttacataa actccttaaa tattgtttta
     7801 aaatccaaat attataaaat ccaaatacta tttcaaggat ttactcatta tatatgtaag
     7861 agctttcttt ataatagctt ctgtattcat accatctttt ttacattttt gaactgcctc
     7921 ttttgattcc gacaaagtat agcctaaagc tacaagtgca tctacagact catcttgtga
     7981 tatcaaggta ggtttttgtg ataataacgt tggttcaaac tctatgttat ttttgtctac
     8041 cttatcctta agctctaaga cgattctttc tgctgtcttt ttaccaacac caggagcttt
     8101 ggataatttt ccaatgtctt cacttaaaat atatgctcct aactgtgctg atgatgcaaa
     8161 tgatagtatt gaaagcgcca ccttaggtcc tattttagat acagatgtta gtaattcaaa
     8221 catcttaagt tcttccctac ttacaaaacc acaaagactc atatcatctt ctcttacaat
     8281 taatttagta tatatttttg tggcttctcc cacttgtaaa ttcattattg tattggatga
     8341 tacatttatt ttatatccta tcccattatt ttcaacaact atactgtcta tataaatttc
     8401 ttctactgta ccttttatat aactatacat acttaccacc tatattcttt agagtttttt
     8461 caagtttgtt tgaatgagca tggcaaatag ccacagctaa agcatctgca acatcatctg
     8521 gttttggaac tttttttaga cttaaaaatg aagttaccat ctgttgaact tgtgccttat
     8581 ctgctctccc atatccaact acaccttgtt ttacttgaag tggagtgtat tcatatacag
     8641 gcttcccatt atgagcacat gcaagcatag taacacctct agcttgtgca actgttatag
     8701 ctgtttttac attcttgtta aagaataatt cttccattcc aacttcatct atattgtaat
     8761 tctttactat tgtatcaatt cctttataca caagttccaa tcttctcgat atattcatat
     8821 gggcaggtgt tgtaactgct ccataatcga ttactttaaa cttgctattt ttgtattcaa
     8881 ttataccata tccaactatg gctatacctg ggtcaatccc caatattatc attttttaaa
     8941 attcccccta aaaataaaag aaataccaaa atgtatttct cataaaaact tatgttctaa
     9001 atcattatat cataaataat attaacaaat cataaaatgc tctatgtaaa atataaaatc
     9061 aaataaaaaa gttatatcaa agcaagattt tacctcaata taacttttat atttattaca
     9121 aataaattat tgtattttta taggaattaa atgtgactac aattttaata ctaatctatt
     9181 taatattaaa ttatttatta ttaacttact tagtatttaa cttgaaataa tattaattta
     9241 ctattaactt gcaaagtctt ctaatcttga ataggcctct tcttcagtat ccataactac
     9301 accttttttg atttcatctt gaactgtttt aggaagtgaa tctactctta tttttgtttt
     9361 ttctactaat gttttacttc cactatcatc atatttatat agtgctatat ttccattatc
     9421 attttcaata gtatatttac cttttatagt agcgttcatt gcttgtacat ctttattatc
     9481 actatattta gattctacca atatgatttc atttttatcc atactcttta tttttttagt
     9541 tggatattct tttttaaaat atgatactat ttctgatttt gtcttatcta ataatgactc
     9601 tggaactatc ccagccattg taagtgttct cttatagtta gtttcatttt cgccttcact
     9661 ttgaagccaa acttcacaat ttttatttaa tccaaaagct tcttttgact tttcgtccat
     9721 attcgcacta gcacttacaa ctttactagt ttgaactcca ttatttttta atcttgtccc
     9781 tatataaact ccgctactta atacaaatac tccaaccagt attaaggcag atattttcca
     9841 aggaaataat tcttttttct tcttttcaaa cattgattac gcctcctttt tctattattt
     9901 tctccaagct gtaagttttt atacaattta ggtaaatttt agaagaaaat atatttatat
     9961 ttcaaattac tctaagaata gatattttct aaaaggtagt tttctcttaa tggttatatt
    10021 cttgtaatta tatggacata atttataata aataatcgtt taggaggtaa taactttgga
    10081 aagaataaac tgtaatgtaa ttaactgttc acataacaac agccatatat gttatgcaga
    10141 taaaattttt ataaatggtg aaaaagctca caatgacaga catacttgtt gtagttcatt
    10201 tttagatgaa tctatttata gtaatttatc tagcaacgtt aataatagaa attgtgagga
    10261 attagtttgt actgtaaaaa attgtaaata caacaaagat ggtagcttat gtacacttga
    10321 taatatattg gttgctcctg aaactgatag agtaaacctt tatattgaaa catgttgttc
    10381 tagtttcgaa tgtaaatagt catagtataa atataagggt gtcaaatgta attaaactaa
    10441 catagtgtag agtatctaca actattagtt tgattatact tgacaccctt atttatttta
    10501 aataaaattt cattattata accatgttta taatgttcta actgtaatta aatttgataa
    10561 atttctataa attggtataa tcaatatgta aattgaattt agcaaattaa tcataaaaat
    10621 aaaatatcga gtatatagtg ttatactaac tagaaatgag gtgcttatga gtacatttta
    10681 tattcaattt ctttattttg taatttatag ctttatagga tggtgttgtg aaactacata
    10741 ttgttcagtt ttacaaaaac aatttgtaaa tagaggtttt ttaaatggtc cattttgccc
    10801 tatatatgga tttggagcat tagcaatagt tgctaccttg actccttttg tacacaatat
    10861 tcctttgtta tttttgttta gtataattat aactagcata atggagtatt gtacgagctt
    10921 tattttagaa aaaatattta atatgacttg gtgggattat tctaaacata aatttaatat
    10981 tcatggaaga gtttgtctgg aaaactcact tatgtttggt gtattatcat tgattgtaat
    11041 gcttatagta catcctattg tggttgactt tattaactct atctctaaaa atgtgctatt
    11101 tatatttgct atatctatag aaatttattt tgttttagat ttagttatca cagtccatac
    11161 aattttacaa ttaaatggta agttaaaaca aatcaatctt ataattaagg aattaaaaga
    11221 taagaaagaa tactacaaac ttattacaca agaaactata gaaaacaaac ttgaatcttt
    11281 agttgaaaat agactggata ctttggaaga tagatttgaa atacttaagg aaaaagaaaa
    11341 ctatcgctat gctaaaaatc gtattgatga gctaaaaaat aaacttaata ctttgctgtc
    11401 taaccacaaa ttacttcata gacgtataat caaagcattc cccaacataa gctctaataa
    11461 gcatacagat attttaaata aaatcaaaga aaatattaaa tcaattaaaa
//
LOCUS       DAEPOZ010000122        11487 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.122, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000122 DAEPOZ010000000
VERSION     DAEPOZ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11487
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.122"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(1..465)
                     /locus_tag="KR440_002367"
     CDS             complement(1..465)
                     /locus_tag="KR440_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699406.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTGKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            735..1673
                     /locus_tag="KR440_002368"
     CDS             735..1673
                     /locus_tag="KR440_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0699407.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWSQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            1801..2814
                     /locus_tag="KR440_002369"
     CDS             1801..2814
                     /locus_tag="KR440_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBH0699408.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            2820..3842
                     /locus_tag="KR440_002370"
     CDS             2820..3842
                     /locus_tag="KR440_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBH0699409.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            3857..4639
                     /locus_tag="KR440_002371"
     CDS             3857..4639
                     /locus_tag="KR440_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0699410.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            4651..5991
                     /locus_tag="KR440_002372"
     CDS             4651..5991
                     /locus_tag="KR440_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0699411.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEDILFILGASKDSIFYAKDYMSVILIGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVTYYFIR
                     GKSTLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     IVQVFPEVFVGIFNKDSDLMDIAVRGISINLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(6086..8366)
                     /locus_tag="KR440_002373"
                     /pseudo
     CDS             complement(6086..8366)
                     /locus_tag="KR440_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            complement(8703..9866)
                     /locus_tag="KR440_002374"
     CDS             complement(8703..9866)
                     /locus_tag="KR440_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBH0699412.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VDYVVIQECGGTLKTVSKGTEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            complement(9879..10154)
                     /locus_tag="KR440_002375"
     CDS             complement(9879..10154)
                     /locus_tag="KR440_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBH0699413.1"
                     /translation="MNAIIHANEKDNLVTCVRPLVKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            complement(10175..11149)
                     /locus_tag="KR440_002376"
     CDS             complement(10175..11149)
                     /locus_tag="KR440_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBH0699414.1"
                     /translation="MHILKSVKKIPGGLMVCPLLLGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            complement(11420..>11487)
                     /locus_tag="KR440_002377"
     CDS             complement(11420..>11487)
                     /locus_tag="KR440_002377"
                     /inference="COORDINATES: protein motif:HMM:NF014950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699415.1"
                     /translation="EILKITRSTLWRKLKEIEDDV"
BASE COUNT         3946 a         1623 c         1810 g         4108 t
ORIGIN      
        1 ttaaaattta gacttctcat aattgtaagg tatactagta ccaacaacta catcttcaat
       61 tttttcaaca ataagccaat catccatatt tccttgatgg tcaaaatcaa gaggtgttat
      121 ttcttcaaca ttttcaaatt caactaagca taaacatttt ttatgccaac gaactttttg
      181 cttttcagat aaatttaatt ttggttgatt ttcatttaaa attttagaaa tttcatcttc
      241 tgttaattta acatagtttt ctactttttt aacagtggct tttgcagata tttttccagt
      301 tcctttttcc ataaaataaa gtaactcatc ttcaaatact ctactatgtg gtatttttct
      361 accagcagca cctctaataa ccattgtttt tgttccagcc aagattttat ctaagacctt
      421 ttctcctttt tttcctgcat tatcacaata tactaaatgt accatgtttt gaatcctcct
      481 tactaaattc attatctata aattgcattt tatcattgtt taacctaaaa gtcattgtgt
      541 ttgtgtccaa ttcttaaaaa aaatactgtt taatataaga aaaccttatc taagctatat
      601 ataattaaga ttaattagga atttttatcc ttttacaaaa taatattgac taaaaataga
      661 aaaatgctat aataaatatg tgcttatgat aatcaatatc atgtaagtat aataaacaag
      721 taggagaagt aatcatgaaa aaaataaaat cacttgcaat atttatatct attataacta
      781 cgttagtact tgttacagca tgttcagata agaatacaga ggataaagat aaatcagaga
      841 caagagttgt acaatctgtt aaaggagagg ttaagatacc atcaaatcct aaaaaaatag
      901 ttgatatatc aggaagtagt gaagaactat tattagcagg ttataagcct gttgcaacag
      961 caaatgtaga ctcatatgaa actgataaat taccaagtta cataagagaa gagcttaaag
     1021 gtgtaaagat agttgggcac tcaatgatgg atactatgga tatggaagct atattagaag
     1081 taaatcctga tttaataata atgagtcaaa gacaagaaaa aatatatgac caattaaaag
     1141 aaatagctcc tgtagttatg atgaaagatt atgcaaatga ctggagaagc aagttaactg
     1201 atgtttcaaa gttatttgac aaagaagaag aagctaaaag ttggctacag aagtatgatg
     1261 aaaaagctac aaagttaggt aaggaagtta tagaaaagaa tggagaaaaa acttatttac
     1321 cagtgttggc aagttcaggt caatttatgg tatttagtga tggtggaata ggtactctaa
     1381 tcaatgatga tatgaaatta gctagaccta agaatatgcc aaaacaggat ggaataacac
     1441 taccaatggt aagtatggaa ggtttaacag atatagatgc agaccatata gttgtaattg
     1501 ctacagaagc agataaaaaa gatttagaaa atagtgctat ctggtctcaa ataagagcag
     1561 taaaagatgg taatgtaaca atacttgatg ctgctccatt tttcagtcaa tcatacaatc
     1621 caattggaaa agagttatta ttagaatcag ttaaaaatga attaacaaag taattcttaa
     1681 cttagtaggt gtgagataat gatattcatt tttcacacct atttttaata aatcttaaaa
     1741 ttaaaatact taatagatga tattatactg acgattaaaa gggaaaagga ggtaggataa
     1801 ttgactaagc tgagtaaaaa aaatataact tatttgttga tattagtaag tatagttgta
     1861 cttgtatttg gaattgtatt atccataact attggggcaa aagatatgaa tctatcaact
     1921 gtcatagata gtttgataaa aatggaagat ggaataaata tgagaatagt aaaggatgtt
     1981 agacttccaa gagcaatagc agctgcactt gttggagggt ttttagcagt ttctggggca
     2041 ataatgcaag gaataacgag aaatcctata gctgaaccat ctgttatagg aattacacaa
     2101 ggagctacat tcgcaatttc gatatcactg gtacttcaaa aaaaacttcc tcaacttata
     2161 catggaagtt ttagtgtaat gatgtttgca tttattggag caagtataag tggattattt
     2221 atatatttta taagttctaa atcaagagga agagttaata atgtaaaatt agctcttgca
     2281 ggagtcgctc ttggtacatt gctaatttct ttggcatcag caatatctat gtattttaac
     2341 ctatctcaac aattaagttt ctggatatca ggagggcttg taggagtgaa gtgggaaggt
     2401 ataaaattgc tatttgtagc tggtggtatt ggatttattt tagctattat tatggctcca
     2461 agaataacta tattaagtct tggagaagaa gtggcaatag gattaggaca aaaaactaac
     2521 tttgttagat ttatatgtat agtacttgta atattaatga ctggagcatc tgtgtcagtg
     2581 tctggtaata ttatttttat aggtcttata gttcctcaaa tagctaaagg aatagtaggt
     2641 tcagattata agtatataat accgagctct ttagtattag gagctgtatt gcttgtatat
     2701 acagatatat taagtaggat gataaatcct ccatatgaga caccagtagg ttctataaca
     2761 gcacttatag gagtgccaat atttatatac cttgttagaa agggggagaa ataggtacta
     2821 tgaaaaatat tataaaaata aattctaaaa cattacttgt tatatcaatt ttaatggtgc
     2881 ttatttttat cactttttta ttgagtttaa atatgggctc attttctata gaaccaatgg
     2941 atgttataaa gactttttta gggcagggtc agcgtaatca tgaaattgca atatttaaat
     3001 taagattacc aagaatagta atagcacttt tggttggttc tgcattatca acagcaggtg
     3061 taatacttca aggagttaca aagaatgatt tagctgattc tggaattttg ggaataaatt
     3121 caggtgctgc attgtttgta gtagtttata tataccttat gaatggtaat gtatatgaag
     3181 gtatgagtaa cttgactgta tttacaatgc ctattgttgc acttatagga gctttatttg
     3241 gagcatttct tatatattct cttgcatgga aaggtggtat aaattcgtca agattactac
     3301 ttattggtat aggtgtaaac atagctttta cttcaatttt gacgatattc caattgaaat
     3361 ttacaacaca agaatttaat agggttatgg catggacatc aggaagcatt tggggaacca
     3421 gttggaaata tgtaatagct gtgcttccat ttatagtgat ttttatgggt ataactattt
     3481 ataaatctaa atatatagat gttttaaatt tgggtgatga agtatctata agtcttggtg
     3541 tagatgtaga aaaacaaaga agaagactta taatatatgc agtaatttta tctggtgtat
     3601 cgacagcagt agcaggtagt atttcatttt taggtttaat agctccacat ataggtagaa
     3661 aaataatagg accaaagcat aaaagattaa taccagtatc agcattgata ggaacattat
     3721 tacttttaat agcagatact atatcaagaa atcttctagc acctatagaa ataccagtag
     3781 gtatagtagt ttctataata ggagtgccgt actttattta tttaatgttg gtaaatgatt
     3841 aggaaagggt gttgttatga actgtataaa tactaagaat ctaaatgtat catatggaaa
     3901 cacagatatt ataaaaaatt taaatttaaa tataccaaaa ggaaaaataa ctaccatcat
     3961 aggggctaat ggttgtggaa aatcgacaat acttaaaact ataggtagaa ttattagccc
     4021 taatagtggg aaaatatata taaatggaga ggacatctac aaaaagaagt ctaaagaaat
     4081 agcaaaggat atggctgtac ttccacaaag ccctcaagct cctagtggtc ttaccgtttc
     4141 agaattaatt tcttatggaa gatttcctca taaaagtgga tttggtaatt ctagtaaaga
     4201 agacagagag attgtaaatt ggagtttaga agttactgga attgcagaat ttaaggatag
     4261 aaatatggaa gatttatcag gaggacaaag acaaagggca tggatagcta tggctttggc
     4321 acaagaaact gatatattat tactggatga gcctacaaca tatttagatt tggcacatca
     4381 attagagata ctaaaattat tagatgaact aaataaaaaa caaggaagaa ctattgtcat
     4441 ggtaatacat gaacttaata atgcagcaag atttgctgac cacatgatag gtattaagaa
     4501 aggtaaaata gtttgtgaag gtgatgcata tgatgttatg acgaaggata atctaaaaga
     4561 attatttaat attgatgcag aaatagcaac agacccaaga acaaataaac caatttgtat
     4621 aacttatgac atggtgtaaa tggtgacaac atggagaatc aagaagcatt aagacatgaa
     4681 aaaatatgga ttttattatt taggtattcg ataccggcaa taatagcaat gatggtaact
     4741 tcactttata atgtagtaga tagagctttt attggttcta tggaaggtat tggctctata
     4801 gctattgcag gtcttggtgt aactatgcca gtatttactc taataatagc ttttggaatg
     4861 ttggtatcag taggtgctag cacaagattg tctataaaat taggggaaag aaatagagaa
     4921 gaagcagaga agatattagg taatgctttg acattatcta taattatctc tttaattata
     4981 actatattag gattagtttt cttagaagat atacttttta ttttgggtgc aagtaaagat
     5041 tcaatatttt atgcaaaaga ctatatgagt gtcatattga ttggaagtat atttaattta
     5101 gttgcatttt ctcttaataa tgcaattaga gcagaaggta atccaaaatt agcagcaagg
     5161 acaatgattg taggatgtgt attaaacctg atattggacc ctatatttat atttgtgttt
     5221 aatttaggta taaaaggagc agctatagca acagtactat gtcaaatagt agtattcttt
     5281 tgggtaacat attattttat aagagggaag tccactttaa aactaaaaaa atgtaactta
     5341 aaattgaata aagatatagt aaagaaggta tttgcaatag gaatgacacc ttttgctatg
     5401 gaagtagcta taagtataac tcacatgttt acaaacaatt ctctaaaggt ttatggtgga
     5461 gatttagcta ttggggctat gacagcactt acatctattt tattgatgtt tatgatgcct
     5521 gtgtttggat tgaatcaagg tatgcaaact ataatatcat acaattatgg tgcaaaacaa
     5581 tatgaacgtg caaagaaaac tttaatatta tctatcatag tgtcagttgt catattgtca
     5641 tttggatttt taatagtaca agttttccca gaagtatttg taggtatatt taataaagat
     5701 tcagatttga tggatatagc agtaagaggt ataagcataa atctaattac attgcctata
     5761 atggggattt ctattgtagg acctgtgtat tttcagtgta taagtaaagt taaacattca
     5821 atgtttttaa ctcttttaag acaatttatt ttatttatac cattaatcat tgtacttcca
     5881 attcaattta atttagatgg tgtttggttg gcacagccag ttgcagattt tattgctatg
     5941 ataatcgtat tattattctt aaaaagggaa tttaaaacaa gccgagtata agaaatttag
     6001 aaaataaaat taaattggaa aataaaaaga cttgtcttta taaaatgatt aaaattattt
     6061 tatagagaca agttttttat tttatttaaa cggaactatc catactggtt tcttctagtt
     6121 tttttccaaa agtcattttc tcaaatattt cttgaggcag aggttttgaa aaaacatatc
     6181 cttgtatcat atcacaatta atttttttaa gataatccat ttgcaagatt gtttcaacac
     6241 cttctgatat actattcata tttaattttt ttgccaaatt aactatagat tctattacat
     6301 gattttcagc aatattatct gcattaggtg agctaaaaaa agcacggtca agttttatat
     6361 gtgtcaactt ttatttcttt taacatattg agtgaagagt atccacttcc aaagtcatct
     6421 agtgaacatt gaaaaccttc ttcatggatt tggtctatta tattaattaa tttttggaga
     6481 ttttcaaata caagtgtttc agttaattca atttctaaga gcttagcagg aatgttgtat
     6541 ttattacgaa gcattctgta tttttctaaa aaatctatat tatttaaatt agcacgtgaa
     6601 aaattaactg aaacaggcat tggagtttct ccattatcta tccattttct tatcatacta
     6661 catacttttt caaaaacata gtggtcgagt tttgtaataa aaccattttt ttcaaagaaa
     6721 ggaataaact cgtctggtgg tataaggcct ttatttgggt cttgccatct tactaatgct
     6781 tctgcaccta caatagtatt atctttgagg cttattttag gctgaagata tactataaat
     6841 tcgttattat ttaatgcttc ctccattcta ttttccattt ctttttcttt aatcattttt
     6901 aaacgctcta aatcactata aaaaacacat gtataaagtg gatgatttcc aatgtttcct
     6961 ttattatttt ttcgtgcaac atttgcacgg tcttgaattg aaactaaagg tagatttggg
     7021 tcatcaattt tatatacacc tatagataga gctataaaat atttacattc ttcattttta
     7081 ttgaaactat taatatcatt ggtgatattt tctatgtact taagaatttc tttttgagga
     7141 atagttttaa taagtacatt aaactggtca ttagaaactc gacaaagcaa ttctcctggt
     7201 tctagataat tactaatgac atcatgtatg tactttaatg ttttatctcc tgcttcacta
     7261 ccaaaagaat cattaataag tttgaatttc tgaatattta tagataccaa tgaatagctt
     7321 tctgatgaag atgactgtat aattttttta gcttcaatat caaaacgtgc tttactgtaa
     7381 cctttagtta cagagtctac ataggcgatt tttataagtt gtgccctgtt cttttgattt
     7441 ataaacataa tcaatataat aagcaataaa aatagtataa caattaaaat attgatgaag
     7501 attgcttttt ttagaagtaa atcaaactgt gcacttgctg tttttgttgg aacaactgaa
     7561 aggaggtata agttttcttc ttgtaatgga agataatgca ttgccttaga aataccatca
     7621 tttaatgtat aatacataga accagttttg ttctttttta agtttatttt catttcttca
     7681 agtttatttt tatcaacctg ttttccttgg tgtttaatca tgtcaaaaaa atttaccaca
     7741 ttttttggag catttttgtt tgctgaactt acaataaaat ttccatcttg gtcgataatt
     7801 tgggaaaatc cttcaccatg aaagctttct acacttaata aatttctcat agtatcaata
     7861 gtattccaag cagcaacagc tccaacaatt ttatcgccat tgtatagtgg tacagaatat
     7921 aaaattccat atgtaccaga ttgtgattta acaggagtaa ttgtttcttt accttgaagg
     7981 gcttttctat aaaagtcttg tcctttaaca ttacctgtat ctttaagggc acttacaaaa
     8041 tttccattag aatctataat acctaaccaa tcaaattcaa ataattttaa tttttttgat
     8101 aagtatctat tgacttcttt ttcactatca ttaatctcaa aataagtatc tcccatagaa
     8161 tatagtgtcc taaatatact tttacatttc acattaattg tagtggaaat actttgtgaa
     8221 acttctacaa gatatcgttc tgtttcatta tgtgttactt tatttagatt cataataaaa
     8281 ttgacacatg ttaatgacac gattaaaagg agtgtcaata gtaaagtagc tattattttt
     8341 ttattttttg tgttttcaaa gttcattctt ttgtttcatc ctccatcctt tatttgttta
     8401 caaataagaa ttctctcaga catattatac caaatatctt ttcatacaaa gatattaaat
     8461 tttatttatc tcactttaca tttagcttta tttatgtctt cttagagatt aatcaagaca
     8521 cgtttattgt ttaaatctta ttttataata ttttgagtaa aagaggttaa aaaaggaact
     8581 gttttcaaaa taattttgat acagttcctt ttcagtatag aaaattttta taggattaga
     8641 aagaactttt agcttatgta tctacataag tattatattt ataaaagata ttttgttaca
     8701 atctaaaatg tagatgaaat tctgtaaatg ccaaattctc tatgaccaag actttcagct
     8761 ttggtatgtt taccattaca gacttctatt atttcattaa atatttcttg acctatatct
     8821 gctattttag caccatctgt tataattcta cctgcattta tatcgatatt gtctggcatt
     8881 ttgttgtatg tatcactatt cccagtgatt ttaataactg gtgaaatagg gcttcctgtt
     8941 ggagtacctc taccagttga aaagattaca atttgtgcac cacctgctac cataccagtg
     9001 atagaatcta tatcttgacc tggagtatcc ataaaatata atcctttttt atcaggaggt
     9061 acaatatcag catattcaag agcacctttg aatggcttat tgcctgcctt atacatacac
     9121 ccaagtgatt tttcttcaat tgttgaaagt ccaccagctt tattacctgg agtaggttga
     9181 ccactacgta aatcttctcc catagcaatt gctctttttt ctacatcact tacaaattta
     9241 agaaacttat ctttcatttc ttcatcttca aaacgggtag ccaacaaatg ttctgcacca
     9301 attacttctg tagtctcact tagtatgcta cttccacctt catctacaag aaggtttgat
     9361 gcaacgccta ttgaagggtt tgaagctatt ccacttgttg ggtcagaacc accacattca
     9421 agtgctaatg taatttcact cataccaaat tcaactctta cttcttttga tacttcacga
     9481 gccattttac ttactatttc agttccctta gaaactgttt ttaatgttcc accacattct
     9541 tgtataacaa cgtaatctac cttttttcct gttgtagcta tttcagatgc taattctttt
     9601 gcttgaatac catcacatcc aagaccaact actaaaactg cggcaacatt aggatttttt
     9661 ccaaatccta ttaatgtctt tgctgtaagc tctatatcag aaccaatttg acaacatcca
     9721 tgttgatgag ggattgctat tgctccaggc acaagtgatg ctattcttgt agctgtctca
     9781 cttgaacata cagatgttgg aataattaaa acatgattac gaataccaaa ctgaccattt
     9841 tctcttaaat aacccattaa tttcatttta acttcctcct attttttatc tccacgacca
     9901 cgtgtacttt caatgttatg tacatgaaca tagtcaccag tttttatgtc aactgttgca
     9961 attcctataa tttcaccgta tttgtataca acatcacctt tttttacatc ttctgtagcc
    10021 atcttatgaa agacaggtat attatcattt actatgtaag actttccatc aattgaaact
    10081 ttttcacctt taactaaagg tctgacacat gtaactagat tatctttttc atttgcatga
    10141 ataattgcat tcattaattt tacctcctta agtattaatt tacttcaata tttttatttg
    10201 ttttaaaaat agattttttc tttttttctc ttttatctag ataggaaact aacattggac
    10261 aacatattgc tgtaacaata atagcagcag caacctgtac tgttgaaaca gctgctattg
    10321 tagctagagt agggtctata gctgctaaag cttcaggagt accagctgca tttccggctg
    10381 ttgtacctgt agctgcacct actgctggat gttctgcacg tataagtttc attacaaagt
    10441 agccacccat acctgttatt atagtaactc ctaaaccaag tacaactcca ggtagtccag
    10501 cagttataat ttgattaaag tttaatgctg ctcctaaagg aaaagcgaag aaaggaatta
    10561 ataatgttgt accaggtgca agaaattctc gtatatcttc atcaaggtta ccaagtataa
    10621 atccaagtag tattggtata agtgctgcaa atagtgctac tataggtaca tttgcaagtc
    10681 cagttactcc aaaagcaagc attgtaaaaa aaggaccatc atttagagat aagatagata
    10741 ttgctccaac atctgtggag tcaccatatt cacctgcaag agcagcaaat agacctccat
    10801 ttgaattagt catggtagca actatggcaa gaggtgtaat accaattata cctttaggtc
    10861 caataaattt actaacaata atacctaata ctgcaccaat tataaatttt gttgctgtaa
    10921 gtactatacc tttagtcaat ggcttaccag cttgcctgat attgatttga gctccattgc
    10981 atagacaaaa tagagcaagt atactcattg caccagtttt aaataaggat gttgtgaatc
    11041 ctccaatttc aagagcttgt ggacatactg tattaaatag tgctcccaat aacaagggac
    11101 agaccataag tcctcctggt atctttttca ctgattttaa gatgtgcatc tagtatcact
    11161 ccctttcatt tttatatagg tattataaaa gcaattacta tgccaatata tagagtttgt
    11221 atttttagtt atcatgatta tttttaataa tcgattgaaa tgctgtagat tacttataat
    11281 taaaggtatg attgatgaga atttcactta caaagattct ttttatcata taattgtatt
    11341 ttttattgaa aacaaataaa cgccttatgt attaaaatta aacataaaac gttttgatat
    11401 atataggtat gtattatttt taaacatcat cttctatttc ttttaactta cgccatagag
    11461 tggagcgagt tatctttaga atttcac
//
LOCUS       DAEPOZ010000123        11429 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.123, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000123 DAEPOZ010000000
VERSION     DAEPOZ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11429
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.123"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(3..1019)
                     /gene="ccpA"
                     /locus_tag="KR440_002378"
     CDS             complement(3..1019)
                     /gene="ccpA"
                     /locus_tag="KR440_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HBH0699416.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(1272..1472)
                     /locus_tag="KR440_002379"
     CDS             complement(1272..1472)
                     /locus_tag="KR440_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699417.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            complement(1506..1685)
                     /locus_tag="KR440_002380"
     CDS             complement(1506..1685)
                     /locus_tag="KR440_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699418.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            complement(1867..2073)
                     /locus_tag="KR440_002381"
     CDS             complement(1867..2073)
                     /locus_tag="KR440_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699419.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            complement(2121..2546)
                     /locus_tag="KR440_002382"
     CDS             complement(2121..2546)
                     /locus_tag="KR440_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699420.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            2718..2945
                     /gene="acpP"
                     /locus_tag="KR440_002383"
     CDS             2718..2945
                     /gene="acpP"
                     /locus_tag="KR440_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBH0699421.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            complement(3089..4093)
                     /locus_tag="KR440_002384"
     CDS             complement(3089..4093)
                     /locus_tag="KR440_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBH0699422.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(4107..5009)
                     /locus_tag="KR440_002385"
     CDS             complement(4107..5009)
                     /locus_tag="KR440_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBH0699423.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKKEK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEELINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            complement(5204..6379)
                     /locus_tag="KR440_002386"
     CDS             complement(5204..6379)
                     /locus_tag="KR440_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBH0699424.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            complement(6466..7311)
                     /locus_tag="KR440_002387"
     CDS             complement(6466..7311)
                     /locus_tag="KR440_002387"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HBH0699425.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            complement(7409..8206)
                     /locus_tag="KR440_002388"
     CDS             complement(7409..8206)
                     /locus_tag="KR440_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBH0699426.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            complement(8225..9235)
                     /locus_tag="KR440_002389"
     CDS             complement(8225..9235)
                     /locus_tag="KR440_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBH0699427.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            complement(9254..10036)
                     /locus_tag="KR440_002390"
     CDS             complement(9254..10036)
                     /locus_tag="KR440_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBH0699428.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            complement(10095..11231)
                     /locus_tag="KR440_002391"
     CDS             complement(10095..11231)
                     /locus_tag="KR440_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HBH0699429.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLKPLASGDKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
BASE COUNT         3459 a         2215 c         1240 g         4515 t
ORIGIN      
        1 ttttattttt ttctttctgt aacactttct ctatcaacaa ttctataagg taattctatt
       61 ttcttatttt caattaattc cttatttatt aattttataa ccattcttat tgctactgct
      121 cccatatcgt atagaggttg atatactgta gtaagtttag gtctatacat tctagctatc
      181 ttaacatcat taaatcctgc aacagatatg tcttctggaa ccttatatcc agcatcacat
      241 atagcattta tagcacctat ggcagcctca tcacctgtta caaaagccgc atgaggtatt
      301 attccatcat ctaatagttc tttcatacta ttgtatccac tctcataatc agttccacca
      361 tacttaatca aactcttgtc taattctata ttgttatttg aaagtgcttt ctcataacca
      421 gcaagtcttt ccatttctaa aacagtatca tctttactcg tcattataaa agctatcttt
      481 tcatgacctt tctttataag atgctctgtc atatcaaaag tagcttcttc gttgctagta
      541 cttactgtat atatatcata atttctagca gttttactta tatatgtcgc tggtattcca
      601 caattttgta tatagttgat atgttcttgt tcaactatcc atgaaatcat aactatacct
      661 tccacttgtt ttgctctcaa tagatttata ctcttaagtt cttgttcctt atcagagtat
      721 gtattcgcta aaagaatatc atagtcatac attttagcaa cttcttctat cccatttaac
      781 acctcattaa caaaagaatc tgaaacttct ggaactatta cccctattaa ttgactcttc
      841 tttgttacta agcttctagc aagtggattt ggtatatatc cagtctcttt tataacctct
      901 aaaacttttt gtttgacttc atcagttaca ggttttgaat catttataac tctagataca
      961 gtagatattg acactcctgc ttgtttagca acatctttta tcgttatatt gcctttcatc
     1021 ttcatcctcc ttattttact aaatttatct aaaactgaac caaatttttg aaggttccta
     1081 ttttaactac atttatataa gtttttattt taccataaaa gcaagtacat ttaaatacat
     1141 tatattttat attataccct atattcaaca aatttaataa tattcattct taatttaaat
     1201 catattaaaa aataaaataa aaaaatactg gtataaaata ccagcatttt tttattctaa
     1261 tgatttaagt attagcagcc gcaacctact tcaccatcaa agaatccacc acatgaacaa
     1321 gttattagca ataatattaa gaaaggtatc catgacatta tgtcaatgtc tctccagcaa
     1381 tcttcaaaag ctaagaatag gaagaatagt actattatcc accatcctcc tccaccaaag
     1441 catccttctt ctccaaaaaa tctatctaac ataacaaagc cctccttttt ttaataactg
     1501 tgttcttatt ctaagcatcc accacagcag caaagtatta ataaaactat taggaatggt
     1561 atccatgcca ttatgtcaaa ttctgcccag ctatctccaa aagctaagaa taggaagaat
     1621 agtactatta tccaccatcc tcctccacca aaacatcctt cttctccaaa gaatctatct
     1681 aacattgtac gttacctcct gttgatattt tgttgagctt ttcgctccta acatatacta
     1741 tgcatttggt ttccattttg ctaatgtatt tttactattt ttttaaattt tttaatttga
     1801 actaaaaaag gtaaaaccat aacttgtata agttactggt tttacctatt taataatcaa
     1861 tattttttag caacaacaac catcatcgaa gttaaataat aacaatacta ttaatataac
     1921 ccatatccat atattgtcat cgcataataa gtcttctaaa atgtctgtgc ataatagtaa
     1981 gaatactata actattatta tcaacatttt attaccaaaa aatccttcgc atcctccaaa
     2041 gaaacgttcc atattgtctt ctaattttgt cataactaat cctccttgaa tcattaagag
     2101 actaagtttt gtctctttaa ttaaatatat gtagtaatag acagataagt tacttctaaa
     2161 aagtttttat tgattagttt atttatttct ttttcaccaa gtttattgta tctatcaact
     2221 tcatccagat aatctttttt ctcctctgat atagtgtaga ttaaactttc tattgatttt
     2281 attttgtact ccttatatct ttctatcaag aaggatgttt ttgaattttc tattcctttc
     2341 tttacatgca ttaaccttga tatgtagtct tctttttcat tagatacata actctccttt
     2401 tcagctacat cttcaaggat taaattggtc tccattacat aatcctctat acgttgcata
     2461 tctaaagtta ttgaattata ataaaaattt actgctataa gtaaaaacat tcctactgaa
     2521 gttagtaata gtcctacata tttcatagca tttcctccta agttatattt gttatcaata
     2581 aagtttattc atcaaataat actttattac tacatttata aaattttatt attttttata
     2641 gattccatat tgaaaaattc ttgatattat attaaactaa cattaaaatt aattaaaact
     2701 gctttaggag gcttaaaatg tttgagaaaa taaaagaaat aatagcagaa caattagggg
     2761 tagacaattt agatgagata actatggaag catctttaat ggacgactta gaagctgatt
     2821 cattagatgc tgttgaaatt ataatggccc ttgaagatga atttggaata gaaataccag
     2881 acgaggaagc tgagaacttt aaatgtattg gtgatatatg taaatatatc gaagaaaaca
     2941 aatagtatcg ttttgtctac aaatataaat agtctgtgta aacttctaca taataaatta
     3001 tattagagtt cgcacagact agattacatt tatttacaat atataaaata cacgctctta
     3061 tatttaattt atatactaag tatataaatt acttttcttc ataaaatgta acattcttta
     3121 ttattatatc tatagttcca tcttgatttt gctttacagt atacttcatt gggtcttcaa
     3181 aatcagttag attccctttt atgtcaaaac cattatccgt ttttatactt ctttttttaa
     3241 gctttttctc aacccatttt ttatctatac taaaactttc atcaagacct ttttcttcca
     3301 tatgctcttt aaagctatcc tttaactcat catctttaat tgaattatca acaaagtcat
     3361 ttatatcaac ttcatgtttt tccttcagtg tataatttaa tatacttctt acatcttcag
     3421 cttgctttat atcattacta agagcattag ttatccaatt ttcagctgta tttttaaata
     3481 ccttggtctt gtacttatca tcatctattt ttttagcatt taaaaattct gttacaaact
     3541 tagattctga cccttctttt tcagcatcct tatctaaaag ccttagatga aactcatcat
     3601 ttataccact taaactaatc aaagctcctt gcttttgtct taaagtctct gggataccaa
     3661 tttcatttga aaccatttga atattaaatt tatcatccac aaattcaatt gaatgagtgt
     3721 acaacttttt ataatctaat ttcagtattg ctacactctt ttcatcttta atagtgtata
     3781 agcaaattgc caaatcacaa gattctagtg cagcattaat tttcataacg tcaaacaagt
     3841 aagatgcaat ttctttagaa tttttcaaaa aagtactttc atcatatatt atttgttcac
     3901 aacagttttt aattaaattg tcattataat ctttaaaaac accttttctt aaatcctcat
     3961 cttttgcaat tctttttata gctttctgga aaaaaccatc tacttcttga ttaactttgc
     4021 cttcaaagtc atttaatatt ggaacatcac tatttttgtc caaaacatgt attataaatt
     4081 tatgtattat catatatatc accccattat cttaatttcg atattaactc tttcatatcc
     4141 tctggtatat ctgctttaaa tttcaatttt tcttttgttc ttggttgttc aaactctagc
     4201 ttgtatgcat gaagtgcttg cctatttatt agctcttcat ctacataacc atacaactca
     4261 tcacctatta ttccatgacc tatatgagcc atatgtactc ttatttgatg tgttctacca
     4321 gtttctaagc ttacttctaa tacagttgca ttttctagtc tttctattac tttatagtga
     4381 gtaacagatg actgtcctct ttcatctatt atcctcttta ttgaatcttc tgtaggtcta
     4441 taaattggct catctatagt tccctcattt tcttttataa ttccatcaac tactgttata
     4501 tacttttttt caactttatt ttcactcatt gctgtagata aagtatgatg tgcataagca
     4561 ttttttgcta ctataactaa ccctgaagta ttcatatcta acctatttac aaatcttatt
     4621 tttacgtttt ctttttgatt atctatatag taactaatac catttgctat tgttttatca
     4681 tagtggcttt ttgttgggtg aactaccata aatggaggtt tattcaccat aattatatca
     4741 aaatcatcat aaattatctg taaatttaaa tcttgaggtt caaaatttgc tttttcctcg
     4801 tctattttta cttctattaa atcaccacta tacaccttta aacttggttt tttatatact
     4861 ccatttacta aaacactctt ttccttcttc atttttgata atgacctaac tgagaaattt
     4921 agcttgtcca ataaaacttc ttttaaagtc atttcttctt catttgtata cgatataaga
     4981 ttatatcttt gattctcttt tttaaacaaa cttttaccta ctttctttta tatcttcctg
     5041 ctttttatat cttcaaaatc atgatattat atattttaca ctatttttgt ttaatagata
     5101 ataattattt ttaactataa aaacctgtaa gaataacaaa tagttattca tacaggttaa
     5161 attcataatt aaatatttaa tttgtaaata ttataatcta aaactatctc ttaactatta
     5221 aagtagttcc cattccacca cctatacaaa gtgtagcaag accagtttta gcatctcttc
     5281 tcttcatttc atataaaagt gtagtaagta ttcttgctcc tgagcatcct attggatgtc
     5341 ctatagctat tgctccacca ttaacattaa ctttattcat atctatattt aagtctctta
     5401 ttacagctac agattgggca gcaaatgcct cattagcttc aactaaatct atatcttcaa
     5461 tagtcatatt agcagcttct aaagcttttt tagttgctgg aactggtcca tatcccatta
     5521 ttttagggtc aacaccagct gttccataag aaactatagt tgcaagaggc tctattccta
     5581 gttcttcagc tttttcttta gccattacta ctagcatagc agcaccatca tttattcctg
     5641 atgcattacc agcagtaact gttccatctt ttttaaatgc aggtcttaac ttagcaagtt
     5701 tttccattgt agtgccaggc ttaatatatt catctttatc tactacagtg tcaccttttc
     5761 ttccttttat aacaacagga actatttctt catcaaattt tccttcagct tgagcttttt
     5821 cagctttatt ttgacttgca agagctaatt catcttgttc ttctctagtt atattccatt
     5881 gctctgctat gttttcagca gtaataccca tgtgatagtt attaaatatg tctgataatc
     5941 catcttttat cattgaatca acaaaagcag catcacccat tcttgcacca tatctcgcac
     6001 ttggtactaa ataaggagac atactcatat tttcagctcc accaactaac attatatcag
     6061 catcacctaa tgctataagt tgagatgcca ttgaaacaga tcttaatcca gaaccacaaa
     6121 ctatatttat agtcatagct ggtttttcta ctggtattcc tgctcctaat gctatttgtc
     6181 ttgctatatt ttgtccaaga cctgctgtaa gtactccccc taaaagagat tcatctatca
     6241 tatctggagt tatgttagct ctttttatag cttctttagc tgctgttacc cctaactcta
     6301 ccgctgaaac tgatttaaat gctcctccaa aacttcctac tgctgttcta gctgcactgg
     6361 caattactac ttctctcata ttcattaccc ccattaaatt agatttttca tatatccgaa
     6421 attgtttttt aaacaatttt atttaaactt ttaattccca aattcttatt tattataatc
     6481 atagaatcct atcttagttt ttcttcctaa ttgattagct ctaaccattt tagctaaaag
     6541 tggatgaggt ctatatttag tatctccaaa ttcagtatat aaaacgttca ttatagctaa
     6601 aacaacatct aatccgatta aatcacctaa tgctagtggt cccattggat ggtttgctcc
     6661 taatttcata gcttcatcta tttcttcttt acttgcaaca ccatctgcat atataccaac
     6721 agcttcattt atcataggta taagtattct atttactaca aatccaggag attcagatac
     6781 atctactggt actttattga tactcttaga taattcaaat actgtatcaa aagtaacttt
     6841 tgatgttaac tgaccactta taacttcaac taatttcatc ataggaactg gattaaagaa
     6901 atgcattcct ataactttat ctggtctctt agtagaagaa gctatttctg ttatagataa
     6961 tgatgaagta tttgttgcca agatagtatc ttctttacat aattcatcta gtaacttgaa
     7021 aacatctttc tttatattca tgtcttctac agatgcttct attattaaat ccatatcttt
     7081 taaatcttca taattagtag ttgaactaac atgacttaat atttctgctt ttgtagcttc
     7141 atccattttt cccttagtaa ctaacttagt taaattttta tctaataaag ctaaacattt
     7201 atctatagca ccttgagttc tactctttaa acatacatca tgtccacaac ttgcaaaagt
     7261 ttgtacaata ccacttccca tagttccact acctattaca gctaatttca tagttataac
     7321 ctcctgttat tttagtattt atattctgtt aactagatta aaatactatt taatctagtt
     7381 aacagcaaat atatttttaa ttagattttt acccttttat aaagttagct tctctctttt
     7441 caacgaaagc tgacattcct tctttttggt ctttagttga aaaacaaaga ccaaataaat
     7501 tagattctat atctattcca gtatttatat cagtttgagc accaagttgt attgcttctt
     7561 tagagtatct aactgcaagc tgagcatttt tagctattat cttagctaat ttctcaactt
     7621 cttctattaa aatgtctggc tcaacgactc tatttactag ccctattttt tcagcttcat
     7681 cagcttttat aacttgacct gtaaagatta attcttttgc ttttgccatt ccaaccaatc
     7741 ttgtaagtct ttgagttcct ccatatcctg gagttattcc aagagttact tctggctgac
     7801 caaatttagc tttagcagat gcaattctta tatcacatgc cattgcaagt tcacatcctc
     7861 cacctaaagc aaatccgttt acagcagcta tcactacttt tttactattt tctatttctc
     7921 caaaagcttt tgctcctaag atactaaaat ctttagcagc tacagcatct aaatctttca
     7981 tgtatgcaat atctgctcca gctacaaatg cctttccttc ccctgtcaat attacaacat
     8041 caatagtttc atcattatta atatctacaa atacttcata aagttcttct aaagtctttg
     8101 aatttattgc attaagggct ttaggtctat tcattttcac tgtacatata tttccatcta
     8161 cttcaacagc tacattctca taaactttaa catcacttgt actcataata ccctccccaa
     8221 gaaattagtt agctaaaact tcaccttttt cttttgcaac acttaactga ctgataagtt
     8281 ctggaagcac tttatgaaca tctccaacta taccaacatc agcatattta aatattggag
     8341 cttctggatt tttatttata gcaactataa actcagcatc ttccatacca gctatatgtt
     8401 gtattgctcc agatatacca catgctatat aaaggtctgg tcttacagtt ttaccagttt
     8461 gaccaacttg tcttgcttta tctaaccaac ctgcatctat agtggcacga gaaccagaaa
     8521 cttctccacc tataatttca gctaattcat aaagtatgtc taagttttct tttccaccca
     8581 ttccacgtcc agcagaaact aatatcttag catcttctat tttaacttgt tttttagctt
     8641 cttttattac ttgtacaact tgaactaatt tatcagcatc attaaattct accttgaaac
     8701 ggttaattac agcttcttta gtttcatcag gttcattttt cttcataacc cctggtctaa
     8761 ctgtagacat ttgaggtctg aaatctttac aaactattgt tgccattata tttccaccaa
     8821 aggcaggtct tgtcattaat aataattttg tatcttcagc tactgcaaga cctgtacagt
     8881 cagcagtaag acctgtatgt attctagcag aaactctagg cgctaaatct ctacctattg
     8941 aagttgcacc aaataataca actatagggt cagctgcttt tattgcttca taagctgctt
     9001 ttgtatatgg ttcagttgta tacactgcta aagcttcatc atctactact attacctcat
     9061 ctgcaccata gtgtgctaat gtatctatta aaccttctac cttactacct aaaagtaatg
     9121 cagaaacttt tgtatcataa tcttttgcta tttctgtagc ctttcctagt aattctaaag
     9181 aaacagtttg aattacattt tctctttgtt ctattactac taaaacgtta cccattcttt
     9241 atcccccctt aaattatatg atatacttct cttttaattt atctatgata attccagctg
     9301 atgttttcgc atcttcattg tatattgtac cagctggttt aactgatttt gtaaatgatt
     9361 taaatacact agttggagaa cctttaagac ctaaattaga agggtcaact tctatatctt
     9421 ttacagtcca tgtttctact acatcatttt cgaaagcatc atatattctt ccaactttca
     9481 tgtatcttgg tgtgttcata tctttaagag ttgttataag gcatggcatt ttaactttta
     9541 agtcatggca acaatcttca aattgtcttt ttactaatac atattcacct tcagttttta
     9601 tttcttcagc atatgttatt gatggaagat ttaaatgttc agctatttga ggtccaactt
     9661 gtgcagtatc tccatctatc gcctgtcttc cagctattat aatatcaaaa tctatatttt
     9721 ttaatgctcc tgctaatgct gatgaagttg cccaagtatc agcacctgca aatgctctat
     9781 ctgttaataa tatacctcta tctgcaccca ttgctaaagc ttcttttaaa gccatatctg
     9841 cttgaggagg tcccattgtt ataacagtta catgagcacc catttcttct tttaatttta
     9901 tagcttcttc taaacctgct ttatcatcag ggtttattat acttggtact ccatctctaa
     9961 ttaaagtacc tgtattagga tctagtttaa cttctgttgt atctggaact tgttttatac
    10021 aaacgactat attcataaat ccttcctcca tatctatttt aatatcgacc tattgtgtat
    10081 attttaatat ttatctattt taataatttt cctgaaataa ccattctttg aacttcacta
    10141 gttccttcat atatttcagt tatcttagca tctctcatca ttctttctac tggatagtca
    10201 cgagtgtatc catatcctcc atgaagttgt acagctttcg tagtaacttc catagctgtt
    10261 tcagctgcaa ataattttgc cattgctgct tctactccat aaggttttcc taagtcttta
    10321 tttatagctg cttgatatac aaggtgtcta gccgcttgta ctttaacttc catatcagct
    10381 aattggaatt gggtattttg gaattttgat aatggtctac caaattgtac tctttctttt
    10441 acatatttaa cagtttcatc aagagcacct tgtgctaaac ctaaagcttg tgctgctata
    10501 ccaattctac caccatcaag agtagacatt gctatcttaa atccttgacc ttcttttcca
    10561 agtaaatttt ctttaggtat tctgcaatcc tcaaatatta attcactagt agctgaacct
    10621 cttataccca ttttcttttc tttaactcca aagctaaacc caggagttcc tttttcaact
    10681 ataaatgctg atattccttt gttcccctta gatttatcag tcattgccat tactacatat
    10741 atgtcaccag ctattgcatt tgttataaat atttttgagc cattaagtat gtattcatcc
    10801 ccgtctaaaa cagcagttgt ttgttggcca gacgcatctg taccagcatt aggctcagta
    10861 agaccaaatg ctcctaattt atctccactt gctagtggtt ttaagaattt ttgtttttgt
    10921 tcttcattac catattgata tataggccat gagccaagag atgtatgagc tgataatata
    10981 actcctgtag taccacaaac tctagacaat tcttcaactg ccattatata tcctacagtg
    11041 tctccacctt ctccaccata ttcttttgga tatggtatac ccatcattcc tgcttttgcc
    11101 attttttcca ctgtttcata aggaaatctt tcttcttcat caagttctgt tgctaaaggt
    11161 ttaacttcat tttcagcgaa gcttacatat agctctttaa gcatctgata ttttttagaa
    11221 tttaaatcca taatagcctc ctcagtattt tataaatatt ttattttcat ttattaatta
    11281 aaatctcggc aacatctata ataaggtatt tgaatatacg tcgagaaatt tataacctta
    11341 atcaaactac gctctatatt tatatgtgat tatactaacc aattatgaca ttaatttaat
    11401 cataaattgt taatttaaaa tcaactata
//
LOCUS       DAEPOZ010000124        11353 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.124, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000124 DAEPOZ010000000
VERSION     DAEPOZ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11353
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.124"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..843)
                     /locus_tag="KR440_002392"
     CDS             complement(<1..843)
                     /locus_tag="KR440_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HBH0699430.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLRPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVN"
     gene            complement(1209..2603)
                     /gene="gltA"
                     /locus_tag="KR440_002393"
     CDS             complement(1209..2603)
                     /gene="gltA"
                     /locus_tag="KR440_002393"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HBH0699431.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLNINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKTKVNA"
     gene            complement(2603..3496)
                     /locus_tag="KR440_002394"
     CDS             complement(2603..3496)
                     /locus_tag="KR440_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HBH0699432.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     ILKLEEGDSHHHSNCGCGGNK"
     gene            4122..4973
                     /locus_tag="KR440_002395"
     CDS             4122..4973
                     /locus_tag="KR440_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HBH0699433.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNDLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            complement(5106..6719)
                     /gene="ggt"
                     /locus_tag="KR440_002396"
     CDS             complement(5106..6719)
                     /gene="ggt"
                     /locus_tag="KR440_002396"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBH0699434.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(6944..8881)
                     /locus_tag="KR440_002397"
     CDS             complement(6944..8881)
                     /locus_tag="KR440_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0699435.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVKTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLIAISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNEIDKLL
                     YKYKKDNKIIFDKTIELIDKDINIEMYYKNGNSYIEDRTSVDIIRESDFKKLRQYQNN
                     NYEELTSKESVYYVSDSYKKMFFKDFEIEKINLYIKDTNEYDINLFKVQKSILEPEIN
                     SQATFDLIVVKDDVFNQLKLHGNSRLIRLIDIKNEKKELDLTLKFKNIVSENMKFTYP
                     FNFTSSIESYNNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDKERYIMLI
                     KLGANNKDIEKIISKQLKIIFLMPLVVGTVHNLFAMSIAQKFIPRSLLVPIIITLVIY
                     FIGYFIYYFLTLKYALNMIKE"
     gene            complement(8868..9635)
                     /locus_tag="KR440_002398"
     CDS             complement(8868..9635)
                     /locus_tag="KR440_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0699436.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALKGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTMDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
     gene            9923..10597
                     /locus_tag="KR440_002399"
     CDS             9923..10597
                     /locus_tag="KR440_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0699437.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            10621..>11353
                     /locus_tag="KR440_002400"
     CDS             10621..>11353
                     /locus_tag="KR440_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0699438.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLINEKISDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSS"
BASE COUNT         3874 a         1815 c         1252 g         4412 t
ORIGIN      
        1 attaactatt agatttttaa agttcttaaa tatattacat tttgatacta aagtccaatt
       61 agtccacttt atgttcgtat attttacatt atataatctc caattatccc aagataaaaa
      121 atcaccttga cattttttat ttatattttt taagatttca cttttagtgg ttcctataat
      181 attcccttct aaatcaagca tcttactttt atcatttaca tattttttgt ttggatgaga
      241 aataatagta taatctttat ctatcattag aaatttaata gaatcatcaa aattgcttct
      301 acttattaaa ttatttacat cttgaaaata aaaagaagca aaaatggtcc caatcatctc
      361 attatttttt acaattggta catatatgat aaatactttg tcacctgtta cattactgac
      421 ttcaatatct gaaactacta cttgctttgt tttaattgct ttctcaaaac taggcttatc
      481 ttttattgag ttaacatttt ccctatatgt acttgaagca tttccttttg tatctgttat
      541 accaatcata aaaagattat attccttttg atatggcctt aataattttc ccttttcaat
      601 tagtgatgtt tcaccattag aaaatctatc atcttgacta agagctttta ataaagatat
      661 gtttacttct aaatagtttt caagttctct ttttgatgcc tcaatcatat ttctagaagt
      721 aatttccata gagttacttg catatatgat ggtacttgtt aaatcaatca aaactaatag
      781 tattaataag attaatatat gcttaaaaac tagcccatca aatacttttt ctcttttttt
      841 catattaaaa taccattaat tacacaattt atttttctat tatttaatgt tagatttgac
      901 aaatctgatt ttattagcca cacattattc actccttaca agacctttat tgtataacta
      961 ttttatcata gttagacaaa gttatgttac ttagtatcaa atattacaat aataactgat
     1021 aaattattaa accatctaaa aaagtgccta catcaaaata gagattgtac taagacttaa
     1081 aagttagact ttctacagga atgaaatttt cattgttgaa atgattaaaa catatttgat
     1141 tttgtacaaa aaaagtgcct catgaactaa aaagttcatt ttgagacacc ctctctttaa
     1201 tttacaaatt aagcattaac ttttgttttt aaatattcat ctattgatgc tgctgctgtt
     1261 ttaccagcac ccattgcaga tattactgtt gctgctccag taacagcatc tccacctgca
     1321 aatacaccct ctttagaagt ctgaccattt tcatctgtta ttaagcatct cttcttattt
     1381 atattaagac tctttgttgt agaagatatt agtggatttg ggcttgttcc aagtgacatt
     1441 ataactgtgt ctacatcaag tacaaattca gaaccttcta tttctatagg tcttcttctt
     1501 ccagaatcat ctggctctcc taattccatt cttatacaaa ccataccttt aacatatcca
     1561 ttttcatcta ctaaaatttc cttaggattt gttaatgtat taaatataat gccttcttct
     1621 tttgcatgat gaacttcttc agctcttgct ggaagctctt tttcacttct tctataaact
     1681 atatatgatt ctgaacctaa tcttaatgca gttctagcag cgtccatagc aacatttcca
     1741 ccacctacta cagcaacttt tttaccagaa ctaataggtg tatcatagtc atctctataa
     1801 gccttcatta agtttactct agttaaaaac tcatttgcag aaaacactcc atttgcattt
     1861 tcccctggta tattcataaa cattggaaga ccagctcctg aacctatgaa tatagcttca
     1921 aatccttcat cttctataag ttcatctact gtaattgttt taccaacaat tacattagtt
     1981 tctattttta cacctaattt tcttatattg tctatttcag gttgaacaac agcttgtttt
     2041 ggaagtctaa actcaggtat tccgtaagtt aaaactccac ctggctcatg cattgcttca
     2101 aagattgtta catcatagcc ttttttagct aaatcaccag cacaagctag acctgctgga
     2161 ccacttccta ttactgctac tttttgattt ttcttaggct ctgtatcaga taaatttatg
     2221 tcattttctt ttgaccaatc tgctacaaat ctttctaatt tacctattga tacagcatct
     2281 gattttattc caagtataca tacaccttca cattgacttt cttgtgggca tactctacca
     2341 caaacagctg gtaatgaact gctcttagct attactttag cagcaccttc tatatcatct
     2401 tctttaattt ttgttataaa ccctggtatg tcgattccta ctggacatcc acccacacat
     2461 tttggttttt tacaagctaa acatctatta gcttctgcca tagcttcttc tttattatac
     2521 cctaagcata cttcatcaaa atttgttgct ctcacagcag gttcttgttc tctaactggt
     2581 acttttactt ttttagcatc cattatttgt tacctccaca tccacaatta ctatgatgat
     2641 gactatcgcc ttcttcaagt tttagaatag ctcttccctc ttgagtcttg tacatagact
     2701 gtcttctcat tgcttgatca aaatcaacta aatgaccatc aaattcagga ccatctacac
     2761 atgcaaactt agtttcattt ccaacactaa ctctacaacc accacacata cctgttccat
     2821 ctatcattat tgtatttaaa cttactatag taggtatatt tagttcttta gttaattggc
     2881 acataaattt catcattatc attggtccaa ttactattgc ttggtcatat tttttacctt
     2941 gattatcaac taaatctttt aataggtctg taactcttcc attaaatcca tatgtaccat
     3001 catctgttga tacatataca tttttagcta cttttttcat atcatcttct aatataatta
     3061 attctttagt tcttgcacca attattacat ctacatctaa tccatgctct ttaaaccatt
     3121 taacttgtgg ataaactgga gcagaaccaa ctccaccagc tacaaataat atctttttat
     3181 ttcttaattc ttctatatct tcatgtataa attcacttgc ttgtcctaat ggccctacaa
     3241 aatccattac aaaatcgttt tcttcaaaca ttgctaactt tttagttgaa gcaccaactg
     3301 tttggaatac aatagttact gttccttttt ctctatcata gtcagctatt gtaagaggaa
     3361 tcctctctcc cttttcatca tttttaatta ttataaattg acctggttga gaagattttg
     3421 caactctagg tgcctctatt tccattaaat aaatactatc tgttaattgt ctcttactaa
     3481 ctattttatt actcatctcg ctattctcca ttctaacaaa tatatttttc ccactttaaa
     3541 tttaattaaa aacataaaca cacaaaacta taactcttta taatataata ttagttcttg
     3601 gttgattgtt ttgtattgga catcccccct atagtaatta taactgccca aactacaaat
     3661 agttgatatt aacaaatttc ttgtaaatgc caataaacac taagtctata tcatacgtct
     3721 aatttgttat agatttaacc taatacacac ttaaagtata ctatattatg ccaaatttgt
     3781 aacaaaaatt ttataaatac ataataaaat ttttttattc aattatttat ttaataaatt
     3841 aacttaggtt ttcataataa cactaatttc tttaaaaact aaacgtgtat aaggctttaa
     3901 tctattgcaa taccagcttt catattatat aaaatatact cttattttat tatatatcta
     3961 aaattttcaa tattcattaa ttatttgaat ttttatataa aatttttaca atttctaaag
     4021 atttatttac ttatgtaata aattataata atattagttt ataaagttaa taattaatat
     4081 aaattaaaat aaagataata taaacatagg aggtacattc tttgaaaaac attatttcag
     4141 caaaagaact tatatcaaaa ctagaaaaaa atgataatct agttgtaata gattgcagat
     4201 ttgatcttat caatagaact tatggtattg attcttataa aaaaggacat atcaaaggca
     4261 gttttatctt agatatagac aaggatttat catctcctac aaaagaacat ggtggtaaaa
     4321 atcctttaca agacccttta atactaaaag aaaaattaga aaaaatgggt gttgataatg
     4381 acactacgat tgtaacttat gatgatggag atttaaatgg agcttgcaga ttatttttcc
     4441 aattaaaaca tttggggctt aaaaatgtct atgtactaga tgggggaata acatctttcg
     4501 taaaggaagg tggagaattg gaagagaaag tgaatatacc aagttgtaca ggtaaggaaa
     4561 ttagaccaaa tataaacaat gatttagttg ttccaatgga atatgttaag tctaaattat
     4621 acaaaaaaga tactgtaata atagattgcc gtgctaatga gagataccag ggtttagttg
     4681 aacctgctta ctcaaaagct ggtcatatac caagtgcaaa aaattatttt tgtaaggatt
     4741 taataaaaag tgactttgaa aatggctcac ttaaagatat agaattttta aaaaaattct
     4801 ttaaagattt aaataattat gatgaagtaa ttttatcttg tggttcaggg atatctgctt
     4861 gtgttaacag tcttgctctt agagaactgg atatacctca caagatttat attggtagct
     4921 ttagtgactg gattagttat gatgacaatg aaatcaaaac tggtcaagaa taaggctcat
     4981 aatctatata aaatcagcat atgatagctt aaattttgta aaggcgtcag tcattgtaga
     5041 ctttcgcctt tactaattat ttcatatact ctttaaattt attatatata ttttagttga
     5101 taatattaat aaactgcaat atgaccatca catcttgatt ccgtcccaca aatataactt
     5161 tcctgttctg ttttaaatat tatttgacct cgtcccatgt cgacagtatc atgaattact
     5221 tttatttcat gacccttttc aattaatgaa tctattatat gctttggcat ctctggttca
     5281 acttctattt cctttccttt tatccactgc catcttggag catctagcgc ctcttgtgga
     5341 tttaatccaa aatcaatcat atttgttaac acttgaatat gtccttgtgg ttgcataaaa
     5401 gctcccatta caccaaatgg acctattgct ttatcttctt tacctaaaaa tccaggtatt
     5461 atagtatgat aaggtttttt aaatggtttt actacattgt ggtgtttagg gtctaaatta
     5521 aaattatttc ctctactatg taaagcaatc ccagttcttg gtatcacaat tcctgaacca
     5581 aaatttatgt aattgctctg tatatatgaa accatatttc catccttatc tgcagtacaa
     5641 aggtatactg taccaccaca atatggctct cctgcagttg gatacaaagc ctctttattg
     5701 tcaattaata aacttctttt tttggcatac tcttggctta gtaattcctg aatctttacc
     5761 ttcatatgct ctatatcagt tacatatgtt ttagaatctg caaaagctaa ttttaaactc
     5821 tctataattt tatgtatgtc ttctacattt tctatatttc cttctaattc caattctttt
     5881 aatatgttaa gtgccattaa aactgttatt ccatgaccat ttggtgggat ttcaaatatt
     5941 ttatatccct tatattctgt acttataggt tctacccatg ttggataaaa acactctaag
     6001 tcactctttc ttatagcccc attaaatttt ttagaatatt catctatttt atcagcaagt
     6061 cttcctctat aaaagctttc agcctgagta tttgctatct cttctaatgt acttgcctgt
     6121 tcttcacata taaatatatc tccagcctct ggagctttac catctttaga aaatgtatca
     6181 aaccatggtt tgaactcttc tccaataagt tctttttcat agagttcata tgattttttc
     6241 cataccttag ctacattagg agatactacg tatccctctc ttgcatagtt tatcgctgga
     6301 gataagcatt ccattaagct aagtttacca tattttttat ttaattctga ccaagctgcg
     6361 ggtattccag gtacagttac tgctccaaac ccatattttg gcatttcttt catattatta
     6421 taatttttta attccatatt ttctggagca aagccactag aatttaaacc atacaatcgt
     6481 ttttcttcct ctatccaaac aagggcatat gcatctcctc caatcccatt gcttgtaggt
     6541 tctactactg ctaatgtggc ggctgttgca actgctgcat caacagcatt tccacccttt
     6601 tttagtattt caagtccagc ttgagatgct aatggtgttg atgttgcaac tgctccattt
     6661 ttagcataca cgacatttct tattgatgag tatttatgtt ggtaggcatt aaacttcata
     6721 aatttcagct cctcgtcatt ttttatttga tattctgctt agatttatta tataacatca
     6781 agtaatgtaa ctgatacaaa atgatataaa atatagactt tttcctaact ttaattttat
     6841 ctttaaataa atatcttaaa ctcaattcta gtattttcta tctgcactta aataagttat
     6901 tttattcaaa acatatatct aattcttttg acttaaaaat aatttattct tttatcatgt
     6961 ttagagcata ttttaaagtt aaaaaataat atataaaata tcctataaaa taaatgacta
     7021 gagttatgat aattggtaca agtaatgacc ttggtataaa cttttgtgct atagacatag
     7081 caaataaatt atgaactgta cctacaacta atggcattaa aaatataatt tttaactgtt
     7141 ttgatattat tttctcaata tctttattat ttgctccaag ttttattaac ataatatatc
     7201 tttccttatc atcatatata tttgataatt gtttaaataa gattatacta cctgtacata
     7261 aaagaaatac tacagacaaa aatatgccta taaacagcat tagaccactt aattttatca
     7321 agttattata actttcaata cttgatgtaa aattaaaagg ataagtaaat ttcatatttt
     7381 cacttactat atttttaaat tttaaagtca aatccagttc ttttttttca ttttttatat
     7441 caattaatct tattaatcta gaatttccat gtaattttaa ctgattaaat acgtcatctt
     7501 ttactacaat taaatcaaac gtagcttgtg aatttatttc tggctccaag atgctttttt
     7561 gtaccttaaa cagatttata tcatactcat ttgtatcttt aatatacaag tttatttttt
     7621 caatctcaaa gtctttaaaa aacatttttt tgtatgaatc agaaacatag tacacagatt
     7681 cctttgaagt taattcttca taattgttat tctgatattg tcttaatttt ttaaaatcac
     7741 tttctctaat aatatctaca gaagttctat cttctatata actatttcca tttttataat
     7801 acatttcaat atttatatct ttgtctataa gttctattgt tttatcaaag attatcttat
     7861 tatctttttt atatttatat aataatttgt caatttcgtt atttacataa gaatttccta
     7921 aatttatggt ataactgtat ttataatttt cacttatgtt cctatctata tcgtaataaa
     7981 gtgatatggt aaaccctagc atagttacac tcgtagctat taatattgct attacagcca
     8041 atacactact attgctcttt aatttaaatc caagttcaga aaatgcaatt aaattctgtc
     8101 ccttataata aaaacttttt ctcttcttaa cagcatcaat aatcatatac atagttgatg
     8161 aaaaaaataa aaaagttcca ggtattattg taactaatat aaaaactgaa agcataatat
     8221 tttcagcaat aaaataacta attgacatta aataagaaat agctattaat aaaacaccaa
     8281 gtacacattt taactttata aattttttaa acttatactt ttctggatat ttattaaata
     8341 actctataat tctcttattt ctgataacta tagtatttct tattccaata actataaata
     8401 ttattaaaaa taccgctagt gtcttaacta aggcctttat acttaagttc atatttacta
     8461 ctatcatttc attcattaat tttacaagca tcatagtcat aagctttgaa aataacagtc
     8521 ctaaaaatat acctattagt attgcaacta tacccatcaa aaaagtttca tataaaaata
     8581 tttttcctat ctgatttttc ctcattccaa gtaaactata agttcctact tctcgcctac
     8641 gtctattgaa aaaaaatgag tttgaataat atataaataa aaatacaaat accactatta
     8701 caattgaact tgtattaata ctcatcctag ttttttctcc aagtgaactc aacgcttcat
     8761 tatactctat agatttaaaa ctaaaaaata caaatacact aaatacaatt gaaataaagt
     8821 acattaaata attaaaaaaa ctattcttta cattttgcat tgcaattcta aataaagtca
     8881 tttttattaa ctcctccaat taaggaaact gtatttacta ttttattata gaactctctg
     8941 ttactttctc cctttacaat ttctgtgaat attattccat cctttataaa gagtattcta
     9001 ttgcaaaaac tcgcacttat agcatcatga gttaccatca atattgtttt attgctgtct
     9061 ttgtttaatt ttaatagaca attcaacaaa tccctagcag atttagaatc taatgctcct
     9121 gtaggctcat ctgcaagtac taatgatggt tttgttgcta tagctctaca tacagctcct
     9181 ctttgctttt gtccaccaga aacttcgcca ggatatttat ctaatataga ttctatattt
     9241 aattcttttg aaatctcttt tattctattt tctattactg atactctttc attttttaaa
     9301 gaaagaggca acattatatt ttcttttatt gtaagtgtat ccaaaagatt agaatcttga
     9361 aatacaaagc ctagattatc acgtctaaaa tctgccaatt cattttgctt cattttaata
     9421 gtattctttc catttatata aatatctcct gatgaaggta aatccattgt agaaataata
     9481 tttaaaagtg ttgatttacc agcaccggaa gaccccataa taccaacaaa ttctccttta
     9541 taaaccttga aagttattcc ttttagagct tcatgttttg aaccttttct tccatactcc
     9601 ttttttatat tttcaacaga taatatttct tccatagtta ataatcacct ttcatcacaa
     9661 ttagttattt atcaattctc acaaatatat tatatatatt aaaatggctt ttaaacattt
     9721 aattatatta atttaaggtt acattttcgt aagtattaat tatatttctt caaatttatt
     9781 gcaattatgt ttaaaatata atttatcaag taaaatatag ttatattaat ataattattt
     9841 taggtaataa ggtagaaata ttttaattta tcaagtagaa tatagttatg ttaatataac
     9901 tattttgaat taggaggtaa gaatgtttaa aataatggta gttgaagatg acgtctcatt
     9961 gaaaaatatc atagcaaagt gtcttactaa atggggacat gatgttcatc aaatagaaaa
    10021 tcttgaaaat ataatagaag agttcaaaaa ctataaccca gaattagttt tattggacat
    10081 aaaccttcca ttttatgatg gttttcattg gtgtaatgaa ataagaaaaa tttcaaaagt
    10141 tcctataatt tttatttctt caagaaattc taatatggat gtaataatgg gtgtaaattt
    10201 aggtgcagat gattatatac aaaaaccttt ttctgtagat gtcttagtag ctaaagttaa
    10261 tgccctttta agaagaactt ataattttgt tgataataac tcaaatcaga ttattcacaa
    10321 tggtgttact ttagatttat caactgcaac aataaactat gaagataata ctattgagct
    10381 aacaaaaaat gaaatcaaaa ttcttcatga gcttatgaaa tataaggggc agatagtaag
    10441 taggaataaa ctcatgaaaa aattatggga taatgattgg tttgtagatg ataatacatt
    10501 aactgtcaat gtaaatagaa tacgctcaaa attaaatgaa attggtttag aagattttat
    10561 agaaacaaaa agaggattgg gttatataat cagttaaaac aattctaagt aggtgaattt
    10621 atgagcttta aagattttct caaggataaa ctaggttttt tagtatacaa ttttactttc
    10681 ttaattttta cagtatcagt tattatattt tctcctgtag atgtttttct gactgatact
    10741 attttatata tattagttgt aaatgttgtg tttttatttt tatatctatt tattagctac
    10801 atcaagaaga acaaattttt agataagata aaaaatgata cattcaaaat taatatgaat
    10861 gatatagaat tagctagatg taaaaatgaa gaaaaaatat acttgaacat tattaaaaat
    10921 catcaatatc aatgtaataa cataataaat aataatatcg agaaatttga agaaaacaaa
    10981 gagattttaa atatgtgggt acacgatgta aagataccta tagctataat aaaactcatt
    11041 cttgaacaaa atgaaaattt aattaatgaa aaaatttcag atgacataga tagtgaaata
    11101 tttagaattg aaaactccgt tgaaaaaatt ttatatttat caagactcga agattttcat
    11161 aaggattttt taattcaaga ggtaaatatt gaaaaaatca ttagaaatat tataaaaaaa
    11221 tactcaaaat attttatttc aaaaaaaatt attttagact taaataattt gaattttaag
    11281 gttttatctg atgaaaaatg gctatacttc atattcgagc aattattgag taattcccta
    11341 aaatactcct cat
//
LOCUS       DAEPOZ010000125        11276 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.125, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000125 DAEPOZ010000000
VERSION     DAEPOZ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11276
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.125"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(250..1521)
                     /gene="sleC"
                     /locus_tag="KR440_002401"
     CDS             complement(250..1521)
                     /gene="sleC"
                     /locus_tag="KR440_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HBH0699439.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIVAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGADTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            1766..2482
                     /locus_tag="KR440_002402"
     CDS             1766..2482
                     /locus_tag="KR440_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HBH0699440.1"
                     /translation="MKLDLFLLAIVPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            2561..3871
                     /locus_tag="KR440_002403"
     CDS             2561..3871
                     /locus_tag="KR440_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBH0699441.1"
                     /translation="MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            3971..4507
                     /gene="lepB"
                     /locus_tag="KR440_002404"
     CDS             3971..4507
                     /gene="lepB"
                     /locus_tag="KR440_002404"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBH0699442.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            4673..5209
                     /gene="lepB"
                     /locus_tag="KR440_002405"
     CDS             4673..5209
                     /gene="lepB"
                     /locus_tag="KR440_002405"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBH0699443.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            5219..5974
                     /locus_tag="KR440_002406"
     CDS             5219..5974
                     /locus_tag="KR440_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBH0699444.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDIRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EVLTLDAEYLVLEINYEDIEDTISKLNNIVREG"
     gene            6048..7715
                     /locus_tag="KR440_002407"
     CDS             6048..7715
                     /locus_tag="KR440_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HBH0699445.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVILIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            8248..9177
                     /gene="glsA"
                     /locus_tag="KR440_002408"
     CDS             8248..9177
                     /gene="glsA"
                     /locus_tag="KR440_002408"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBH0699446.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            9275..9838
                     /locus_tag="KR440_002409"
     CDS             9275..9838
                     /locus_tag="KR440_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0699447.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            10326..11162
                     /locus_tag="KR440_002410"
     CDS             10326..11162
                     /locus_tag="KR440_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBH0699448.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHIGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
BASE COUNT         4292 a         1169 c         2122 g         3693 t
ORIGIN      
        1 attaaactta aaaataaata taatatttta aagaaatcta agttgctaag cactgttttt
       61 aagaagctta aaatccccca tttaaagttt taagattcta aaaaaacaag ttataactaa
      121 aaaatggctg ttaagactac aatacttata agtaaaaata cttatggtat atattagttc
      181 ataacagcca tttttatttt aaattaataa ttttattcaa taatttgagt ttatgttcta
      241 aatcaagctt taaattaaag gatttaaaga agctattcta gttgtagcta cataaactct
      301 ggatatttca taccatgttt taaaatctac tactccgctt tgaggtaaac caaagattcc
      361 ttggaatatc ttaacagcgt tttctgtatc tgctccatag ataccatctt ctataacttt
      421 tggtacagct ggataagaat tagatatagc atttaactga ttttgtatgg ttctaacata
      481 ctgcccagaa gaaccaactt gtagtgtagt accagggaat gacactggta caccacttac
      541 tataggtgct ctttcaaata ctatctcatc tccatagaaa tatcttagga tactctcata
      601 gtcatatcct tggtcaccta agtctttact gccccattga gtcatttggt caggacattg
      661 agatttttgt ccatcacaat attgtgctaa tagtggctgt cttgatgtag gaggtctttt
      721 tataaaagta ttaaaaatag catctacaac tacgttaatt ggttcaaata agtttctatt
      781 atttataaac ttatggtcgt atgctgttgt tgaagttatt gtaaagttat atcctttatt
      841 tctataccat tcagtaaaga ctctatttaa agtaaatgat attatagcta ttacatttgc
      901 atagattgtc tgttcaggcc aagttgaata tatttcagat gacgctatgt ttttaatgta
      961 ctctttaaat ggaatccaat agtttggagc tgatgaatct tctggcaaac catcatgaac
     1021 tactataaat tcaggcacta cagggttatc taatacaacg aaaccagtag gaggtggtaa
     1081 tggttttaag ttactttctg gaatcttagg aggataagta ccatatagag tatgttctcc
     1141 tatgtcaaaa atcaactcac tttgtcttga gaaagaacgc ttacttcttg ttctaggaga
     1201 catgggaact ccttgtcttg ctacaattgt agctaacaac tgcgttcctt ctatgacaac
     1261 tgtctcatat ccatctgcta tggcttccac tatatactgg ctataaggtc taacatcaga
     1321 tggctgtaga gagtaatcta catctggtgc tggtaatact aaaccagtca cttgaccaga
     1381 ttcattactt cttaggtttt ggtataaagt tgatgagctt tggctaccat tagacattga
     1441 atatatgttt actactgcat tttgaatagg tctattgtta gtagcatcaa ttatgcttac
     1501 tgttaagaaa ccatcttgca tcaaatcacc ctttctttaa atgaatttag ttcccttata
     1561 tttcaagata tgaatatagc ttgaaatttg ttaaataata tagatataaa tttaaatttt
     1621 tttgagtatt tgagtatatt tgtatttaaa taatttaaaa taatttaatg tatattttag
     1681 tatgtattta aaacacaaat aagctaaaaa taagtagatg atataaatca taaataaatc
     1741 aaaaattttg aaaggaaaat aatctatgaa gctagattta tttttattag caatagttcc
     1801 tatattgata gggatgtttt ggataaggtc taaagataga tattgtagag aacccctaat
     1861 ccatctaatt aaattttttt taataggtgc ttttctaagt gttataatta tacttttaga
     1921 aaatttactg atgaagttta atgtatttga agggtattca gaactaatat atgtatcatt
     1981 tgtagttgca ggacttgtgg aagaaggtgt aaaagctctg atactgattc ctgccctaat
     2041 taaagaaaaa cactttactg aaaagttaga tggtattatt tactctgtat ttttagcttt
     2101 aggatttgca acaattgaaa atatggttta tattttttct gaaagtagaa atctagcact
     2161 gcaagttggt attaataggg cagttatctc tataccagct catgtaatgt ttgcaattac
     2221 tatgggctac tatatttcta agtataaatt tgagggaaat aaaaataaaa gacgtgagta
     2281 tttgtttatg gcagtgttaa ttccaatttt attacatgga gtttttgatt tcatattgat
     2341 gatagaatat agatgggcta ttatattact tatagtttat gtaataattt tgtggaagat
     2401 aaatcttgat aaacttgaga aatatatgaa tcactctaaa aaagtgtttt tcggtaatct
     2461 taggaaaaag aagaagaaat aaaatttaaa ggaaagacat aaataattaa agtataataa
     2521 atgacacaca agtaaagcag attgaagggg gattatagat ttgacagaat taccaaagga
     2581 atttttagaa gatattgaag aaatactaaa agatgaatat gatgatttta taaaaagtta
     2641 tgaggaaagt aagactactg ggcttagaat aaatacttta aaaattgaca agaaaaaatt
     2701 atttgatttg gagttgtttg atttgaatca aataccatgg gcagaagaag gtttttatta
     2761 tgatgataaa attgacagac ctggtagaaa tccactgcat gaggctggag catactattt
     2821 gcaagagcct tctgcaatga gtgtaatacc aaaggcagat attaaagagg gtgaaaaagt
     2881 tctcgatatg tgtgctgctc ctggtggaaa atctacatat atactatcta agctaaacga
     2941 tacaggttta ttggtttcta atgaaattaa tgctagtaga attaaggctc taggggaaaa
     3001 tttagagcga tttggagcga gaaattgtat tataacaaac accgattcta atagcttaag
     3061 aaagacgttt aatgggtatt ttgataaggt tattatagat gcaccttgtt ctgggcaagg
     3121 tatgtttagg aaggatgagg ttgccatatc agattggagt tatgcaaagg tagtagaatg
     3181 tcaatccatc caaaaagaga ttatcagaga tggatataaa atgttaaaga aggatggcat
     3241 tttaatttac tcaacttgta ctttttcaag ggaggaaaat gagcatgtta taaatgagtt
     3301 tatcagtgaa tatccacatg cagaacttat tgaaatggaa agattatggc ctcataagat
     3361 aaagggagaa ggtcattttg ttgccaaaat aaaaaaatta gaagaagaag aatgtaatgt
     3421 caaagaatta aaaataaaaa atttagataa agaaataaag gattataggg agtttgaaaa
     3481 gaaattttta aaggttgatt tttatagtaa taaaaaaaat aaattttatt taagaggaga
     3541 aaatttgtac ctacttcctg aaatatatcc agatacgaaa aaactaaaag tattgagata
     3601 cggtttgcat ttaggggttt taaagaagaa tagatttgag ccttctcatg cactatctca
     3661 ttacttaaaa gttgaagatg taaaaaatgt tgaaaacttc agtgtacaga gtgaatcaat
     3721 tcttaaatat ttaaaagggg atgttgtgaa ttctaatgat agtagaggat gggttttggt
     3781 ttcggttgaa ggaatacctt taggttgggg aaaagaatct tctggtgtaa ttaagaatca
     3841 ttatcctaaa ggtcttagaa aagtatttta aactattaga aaaaagaagt ataatattta
     3901 tattgtaggt ttatggtaga ttattttgct gtagattgac ttaatattta acacagagga
     3961 gacgatttta gtgggtgaag cagttaaaaa agaagttgta gaatggataa aagtgattgt
     4021 catagctctt gttttggcat ttgcaataac tcgttttata gtgccaacaa tagtcaaagg
     4081 agaatcaatg tatcctacat tagttgaacg tgattatttg atagttaaca gaattgcgta
     4141 caaggtagga gagccaaaat acaaagatat aatagtattt aaaaccgact taacagagga
     4201 aaatggaaag aaaaaagatt tagtaaaaag agttatcggg gttcctggtg accatgtaaa
     4261 aatacaagac tccaaggtat atgtaaatga taagttgtta gatgagactt cctatataca
     4321 taataatcgt actgatggag atattgatat cgtagttcca gaaggaaaat tatttgcaat
     4381 gggagataat agagaaaaaa gtttagatag tagatacgat gaggttggat tggtcgacga
     4441 gcataccatt ttaggaaagg ttctagtcag attgtatcca ttttctaaga taggaactat
     4501 tgactaaaca tataaaattt ggagcaatcg ctgattagct gaaatcacca gaccatacac
     4561 aatagttgtc tatataaaaa agacaacacc ttatttagta gacttatcta aaagatagtg
     4621 tttaagagac attatcaata aagataatat aactataaag gagaatttgt gaatgaatga
     4681 aactattaaa gaagagattg tagagtggat aaaaataatt attactgcac ttttttttgc
     4741 atttattata actcgtttta taaaaccaac actagtaaat ggagaatcaa tgtacccgac
     4801 acttaaatca catgattatt tggtagcaaa taggatgaca tataagttat cagaaccaaa
     4861 atgtggagat ataatgatat ttaagactga tttattacaa gagaatggaa ggaaaaaaga
     4921 gcttgtaaaa agggttatag gtgttcctgg tgaccatcta aaaattaagg attctaaggt
     4981 ttatataaat ggtaagttat taaatgaagt ttcatatata catgataatt atactgaagg
     5041 cgatattgat atggtgattc ctaagggaaa agtatttgcg atgggagaca atagagaagt
     5101 tagtttagac agtagatata aagaagtggg attggtagat gaagaaaata ttaaaggaaa
     5161 agttatttta agagtatttc cttttacaga tataggtatt tttgagtagg tgatttgtat
     5221 ggcagttggt atagcgtcct tattgtttaa tattgaggag gctttaaaca tttgtgagag
     5281 tataaagcaa ataactcatt tagagattgg aattgacaat atatctgaat gtagcgagtt
     5341 atgtaaatat aaagagagaa tttctaagtt aggattatct atagggatac acttgcctat
     5401 ggaattaaat acttgcgaaa acatagagta tatacgaaat tcatggataa attttattga
     5461 gaagatagaa tttgaactaa aaggatttga tataaggtat tttaaccttc atttgggata
     5521 tgttatgaca aatagagtta gaaagaatag agacaaatat ttaggaaata gtgtggattt
     5581 tttagataaa ttaaatacaa attcatatgt atgtatagag aatacttatt caaaaggtgg
     5641 ggatttttca aatattggaa atatatctta tgattttgaa tatatattta agaggattaa
     5701 aaattctaag atttgttttt gttacgatac tggacactgt ttaattgatg aggatgctta
     5761 tgtaaagaac ttaaaggata aaataagact gatacactta agtgataatg atggtatcaa
     5821 tgatacacat gttggtatag gaagaggaat tttatctgaa gaggggatta aagaggtctt
     5881 aacattagat gcagaatatt tagtattaga aataaattac gaagacattg aagatactat
     5941 aagtaaattg aataatattg taagagaggg ttaaatacct ctctttatct tgttgatatg
     6001 cctcattaac taaaatttat attcatcaaa aggaaggaga tgttgaagtg aaaaaaataa
     6061 acttaaaaat agaaggaaaa gataaggaat attcattaga agaaaattcc cctggaatca
     6121 gattgggaga tatagcgaaa gaattttgtg atgagcataa gggttatata acacttgcag
     6181 ttgtagacaa taaactaaaa gagctaaatt gtagagttaa gaaggattgt gagataaatt
     6241 ttttagacac tactaatgaa gatggagaga gagtttactt tagagtcatg tcatttatat
     6301 ttgtaatggc ttgtagagaa atcttttggg atagcagagt tacaatagaa cattcactat
     6361 cagatgggct atattgtgaa gttcatatag atagaaagct taaagaagca gatgttgaaa
     6421 atataaaaaa caagatgaaa gagatagtaa acaacgatta tgttatagaa aaaattgaag
     6481 ttacaagaaa tgaagctatt tctatatttg aaaataatga aatgtatgaa aaggcagagc
     6541 ttttgaaata caaagagtat gatagtgcta aagtatataa gtgtagaaac tatatagacc
     6601 atttttatgg ttatatgttg ccatcaactg gttatataaa atcatttgat attaagcttt
     6661 ataatggagg cgttataatt ttagggccta gtgaagaaga taagactctt cctatgaagt
     6721 ttgtacctca acctaagcta tcaagtgtat attatgaagc agaagaatgg tcaagactta
     6781 tgggaattga caaggtagta tctcttaaca aaataataga aaacaatgaa tatggagata
     6841 taataaggac attcgaagca ttgcatgaaa agaagttatc ccaaatagcg gatatgataa
     6901 aggatggcaa taagagagta attttaatag cagctccatc ttcatctgga aaaacaagtt
     6961 ttgctcacag attatcaata catcttagag taaataattt aaatcctatt tcaatttctt
     7021 tagatgatta ttttatagat agaaaacata ctcctttaga tgaatatgga aattatgatt
     7081 ttgaatcaat ttatgcaata gatttagaga agtttaactt agacttaaag aaacttttag
     7141 ctggtgaaga aattgatgat ataagattca attttaaaga aggtaagaga gaatacactg
     7201 gtaaaaaaat aaagatagat tcaaatcagc caattatatt agaaggtata catgggctaa
     7261 atcctatttt aacatcatct ataccagatg aagataaatt taagatatac ataagtgccc
     7321 ttactcaaat aaatttggat gaccataatc gaatccctac tgctgatttg agaatgataa
     7381 gaagaattgt tagagactat aattttagag gatatagtgc tgataatact attttacaat
     7441 gggcatcagt tagaagagga gaaaagaaga atatattccc gtaccaagag gaggcagatg
     7501 ctatatttaa ctctgcttgt gtatatgaat tagcagtttt aaaacgattt gcgaaacctt
     7561 tgttggaaga gataaaggaa gataatcctg cacatattga agctactaga ttactaaagt
     7621 ttttgcagta ttttgttgaa ttggacgata cttctgacat accaggtatt tcaattctta
     7681 gggaatttat tggaggaagt aaaatagttg attaatatgt gtattataga ttgttttatg
     7741 aagattaaat ggcatagcaa gtagtgtgat tggtttatgt taagtatatt aagtgtaaag
     7801 tggagaatta aattgataca gattttaaag tttgagttga tatataaaaa ggcttgtttt
     7861 atacttttgt atgaagcaag ctttttattt taaattttac ttttttgttt aatattttgt
     7921 tagtttatgt gataattttt taaaagagta aaagagtaaa agagtaaaag agtaaaagag
     7981 taaaagagta aaagagtaaa agagtaaaag agtaaaagat gttaggttag aaatttttga
     8041 cgttataaga atttaaatta ctggattatt agattactaa attactagat tattaaatta
     8101 ttaagctatt taaactgttt aaattgtttg aaaaatgaat tgattcttgt agaaaatagt
     8161 tttaaagtat aatttttttt aacactgttt tattatagta caaacttgtg ctaaaataga
     8221 atatataaca taatataaga ggtgcatatg aatacgttag atgagacatt attaaaagaa
     8281 attataagtt ctaataagaa gtatactaat tatgggcaag tcgctagtta cataccagaa
     8341 ttaaaaaacg ctagaagaaa tgatttagga atttgtatca tagatagcga aaataatctt
     8401 tatagtgctg gaaactgtag cacaaagttt actatacaaa gtatttcaaa accaattgtg
     8461 cttgctatgg cacttatgga taatgattgg gaagacgttt tttcaaacgt tggaatggag
     8521 ccaagtgggg acccatttaa ttcaataatg aaacttgaaa ttaatgacac aaaaaaaccc
     8581 tgtaatccaa tgataaatgc aggggctata gtaactactt cccttataaa tgggagttgt
     8641 ttagaagaaa aagaagaaag aatgcttagt ttctttagaa agttagcaaa aaatgataat
     8701 ataggaataa actatgatgt ctataagtct gaaaaaatga ctggagatag aaacagagct
     8761 atggcttact tattgaaaag tgatggattt atcagaggga atgtagaaga tgttctagac
     8821 ctttatttta aacaatgctc aattgagata gattctgtag atttagcgag aattggaata
     8881 aatcttgcaa attatggagt agatatagaa aatggtgagc atttaatgag tgaaatggtt
     8941 tctaggattg tcaagacatt tatgatgact tgtggaatgt atgatgcttc tggggaattt
     9001 gctattaaag ttggcattcc tgcaaaatct ggagttgggg gtggtataat ggcttctgtg
     9061 ccgggacgca tggggattgg tgtttatgga cctgcgcttg acaaaaaagg taacagcgtc
     9121 gcaggagtta aagttttaga ggagttgtca aataaactca aattaaatat tttttaaaat
     9181 attaagaaag gatttttttg acaacaacat ataatttaat atataattaa attgtaaaga
     9241 aatatgaatt aaataaattt tttaggaggg gtcaatgaaa gaggtattgg tattacgaga
     9301 tttggagtgc ataaaggcta ttgcgcatcc acgtagaata gatattctaa aagcatttga
     9361 caaatcgcca ttgtcagcaa aacaattatc tcaattattg gaagaacctc atgcaaaaat
     9421 aaactaccat ataaaaatgt tatacaaagt tggtatttta gaattagttg aggaaaaaat
     9481 aaagtcgggg attgtggaaa agtactatta tccaagtgca aaaaatgtgg ttatagggaa
     9541 taggatactt aatttttctt tagacaatgg ggaagaaaaa gaagaattgt acatatcaaa
     9601 atttgaaaat atgagtgaag ttttttataa ggcagcagag gaagatgttt tagaaaatga
     9661 aaatatagtt gattatcatg atatttctct tactcatgat gaattagttg aacttagtga
     9721 cactatgaag tcaaagatag atgaaatttt aaataaaaga caacacaatg ttgaaggttc
     9781 taagtatgat atagctatgg ttatagttcc tactcttgag gaggaatgtc ctagttagag
     9841 aattaattat ggattaatta aagatataat attagagatt gaacatcaaa atttacttaa
     9901 taatcaaaaa atagtttttg gtatgggtaa aacaatgatt tttaatctct atttttttgt
     9961 tttcttatac ctattgtatt tgtaaataat atgtatagtg taagatgtat acactatctg
    10021 agaagtgctt agataatata caaagtatgt aagattataa tatttgaaat tgattaaaat
    10081 gggtataatt aaagtgtaaa tattaaaatt aaaaattaat ataaattaaa tcaatgctag
    10141 aaatatgtta aaagaggaat gagattagtt gggatagaac atcccttaga gcttatgaat
    10201 ttttagtgaa aaagtactat caaataggaa tctccatctt catatgttgg ctaaatatag
    10261 ttaaggtgga gtagttcaaa attaaaattg agtacaagtc gtagtacaaa taaaaggaga
    10321 ttaatatgat taaattagga acacatcttt caatagcaaa aggatttgct aatgcggctg
    10381 agacagctgt atatattggt gcaaatacat ttcaattttt tagtagaaat ccaagaggag
    10441 gaaatgctaa agaatttgat gaaaaagata taattaaatt tcaagaaatt agaaaagaaa
    10501 ataattttgc accactgctt gcacatgcac catatactat gaatctgggt ggtacaaagg
    10561 atgatgtata cgaatttgca acaagggtga tagatgagga tataagaagg atggactcat
    10621 taggaattga atacatgtgt tttcatcctg gaagtcatat aggtggtggt gtagattttg
    10681 gtatagatag aatagtaaaa ggattgaaca atgccataaa gggtgatgaa aatataacta
    10741 tacttcttga aactatgtcg ggaaagggaa ctgaaatagg ttttaaattt gagcaattaa
    10801 aaagtataat agacaaaatt gagcataaag aaaaaatagg tgtgtgtctt gatacatgtc
    10861 atattttttc agccggttat gatatagtga atgatttaga tggggttttg gaagaatttg
    10921 ataaggttat aggcatagaa agattgaaaa ctgtccatct aaatgatagt atgatgccat
    10981 ttggagacaa aaaagacaga catgctccta taggggaagg aaagatagga ttaaaagcat
    11041 taattgattt tatggaacat cctagtctaa aacatttacc atttttctta gaaactcctt
    11101 ttgatgatga aggtcacaag agagagctta aaatgataaa agaaatacta tctagtaaat
    11161 aaacctttat taaaaatatg tgaaatactc cgccctcaac ataattaagg tggagataca
    11221 atcatgaaat gtgataattt aaattaatgg ataggaataa caaaaattgt atacaa
//
LOCUS       DAEPOZ010000126        11141 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.126, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000126 DAEPOZ010000000
VERSION     DAEPOZ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11141
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.126"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..687
                     /locus_tag="KR440_002411"
     CDS             <1..687
                     /locus_tag="KR440_002411"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699449.1"
                     /translation="SQYRSQYSFRHESNINNTRINESMVKKNETVGKDTYLSNIMKQK
                     QELNDRIRDLKYRQEVYTKKINEAIKNLYKSEIRETTNNFSNIEIGIKNSIIEDKNKS
                     TMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDYKEDSNKKEKVS
                     EDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKDAEEYKQNSKTNIFDKTD
                     EQKKHINVLI"
     gene            789..1337
                     /locus_tag="KR440_002412"
     CDS             789..1337
                     /locus_tag="KR440_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699450.1"
                     /translation="MEGLGFYAAALSGSSYQRIGFGKLDPIEVIADGDWISYKQAQDT
                     LTVIRNFLNSFDWRNASEMERANRAAKLVTEAKYVDSKYCNIVYGNLVDKRGVCGSFA
                     SSFHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRSDGSEISAFGGALDFDYRKLK
                     DATREMTTYYDAKALSILGFNQ"
     gene            complement(1738..2694)
                     /locus_tag="KR440_002413"
     CDS             complement(1738..2694)
                     /locus_tag="KR440_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBH0699451.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDNNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSYGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIQFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENKSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            3397..4392
                     /locus_tag="KR440_002414"
     CDS             3397..4392
                     /locus_tag="KR440_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0699452.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNHFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKTSVIVLKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            4370..5233
                     /locus_tag="KR440_002415"
     CDS             4370..5233
                     /locus_tag="KR440_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBH0699453.1"
                     /translation="MSIMSNYNEASSNYSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFRMIIENIVEFITGTMIPL
                     VLLPETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            5230..6024
                     /locus_tag="KR440_002416"
     CDS             5230..6024
                     /locus_tag="KR440_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBH0699454.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISKLNSEITLVMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            6309..7688
                     /locus_tag="KR440_002417"
     CDS             6309..7688
                     /locus_tag="KR440_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0699455.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTVSGMVFFSKNKELLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTIFCIC
                     KYKNRYKYM"
     gene            8143..8856
                     /locus_tag="KR440_002418"
     CDS             8143..8856
                     /locus_tag="KR440_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0699456.1"
                     /translation="MDTYFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSAVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(9128..10666)
                     /locus_tag="KR440_002419"
     CDS             complement(9128..10666)
                     /locus_tag="KR440_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBH0699457.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVEEL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
BASE COUNT         4038 a         1278 c         1685 g         4140 t
ORIGIN      
        1 agtcaatata gaagtcaata ttcatttaga catgaaagta atataaataa tacaagaata
       61 aatgaaagta tggttaagaa aaatgaaact gtaggaaaag acacttattt atctaatatt
      121 atgaaacaaa agcaagaact taatgataga attagagatt taaaatatag acaagaggtt
      181 tatactaaaa aaataaatga agcaattaag aacttatata aatcagaaat aagagaaaca
      241 actaataact tttctaatat agaaataggt attaaaaata gcattataga agataaaaat
      301 aaaagtacaa tgttagatga aaattcaact tatctaaata caaatgatga aaaagaatct
      361 ttaatcacta aagagtctaa tgaaaaaatt gaagaagaaa tattaaatga tgaaaaatta
      421 gaagagttag aacaaaaaaa ggattataaa gaggattcta ataaaaaaga gaaagtatct
      481 gaggacttat ctttagtagg taaaactcgt gaagagcttg aaaatatgct taaaaatttt
      541 ataaatttaa cacaagaaga aataatgaaa cttgagtcga gaatagaaaa gttagataaa
      601 gatgctgaag aatacaaaca aaattcaaag actaatatat ttgataaaac agatgaacaa
      661 aaaaaacata taaatgtact gatttaaaca atatgtttta aaaaaataag tttaatttac
      721 tctatggagg aaatccaaat ggagttgagc ttcgagcaga tggtaaatat tattatataa
      781 ataagggtat ggaagggcta ggattttatg cagcagcatt gtcaggtagc tcatatcaga
      841 gaataggctt tggaaaactt gaccctatag aagtaatagc cgatggagat tggatttctt
      901 acaaacaagc tcaagataca cttacagtaa tcaggaattt tttaaatagt tttgactgga
      961 gaaatgcatc tgagatggaa agagctaata gagctgcaaa attggtaacc gaagcaaaat
     1021 atgtagactc taaatattgt aacatagtct atggtaatct ggttgataaa cgtggtgtat
     1081 gtggaagttt tgcaagtagc tttcatttat taacaaggtt aatgggaatg gattcactat
     1141 ctatattaaa tccatcatta aatcatgctt ggaactatat acaaattgat gggaaatggt
     1201 ataggtctga tgggtctgaa atatcagctt ttggtggagc attagacttt gattaccgta
     1261 aattaaagga tgcaacaaga gagatgacta cttactatga tgctaaggca ttgtctatat
     1321 taggatttaa tcaataaaaa taggggaggc tttataaaga gccttttttt attgatttga
     1381 aatttaataa ggaattttat atgaaaattt aatcaataaa agcaaaaaag gctctttata
     1441 aagcctttct aagtgttttt ttaagtaaaa ttttttatat caacatcagt tttaatcaaa
     1501 aaatataaca ttttggactt acttttaaaa ataaaattat taaaatataa tattaatcat
     1561 gttaataatg tttttagttt attatattct ataatagtgg tcatgttgac tattgtagtt
     1621 ttattaagaa tagttggttt atataaatat atgcaaagat agcttgttgt ttactttact
     1681 tattagtata taggacaaga tactctaata aatttgccta ttttaatatc ttttgtacta
     1741 aaaccaacta aagtttcctt tattatttcg ttttgaataa tacaaagcat aatccgattg
     1801 tttgtataaa gactggaaag tatcagattt attttctaat ttagatatac ctatagaaca
     1861 agaattactc tctagacata attctaaaac tttctttgct ttattttcaa cattctcttt
     1921 tgattgatta ctatattcaa aaacagcaaa ttcatctcca ccaagtcttc caataatagc
     1981 atcatttacg aatactttag tcaaaatatt tgaaaactgt attagtattt catctcctcg
     2041 aacatgacca aagatatcat ttacctgttt aaaattatct aaatcaaaga tgtaaaagta
     2101 tccatatgaa acagttttaa gcatttcttt tatgtgtact tcaaaaaaat gtctattgta
     2161 tagtccagtc ataaggtctt tttctgattt tagtttaaat tcatttgcca ttgctagtgc
     2221 ttcttgtgct ttttcagtta ttgttttagg gtaatactca tctttcattt ggtcaaagtt
     2281 tggaatagaa tggtgaacat ggataagttt aatcatatca tctgtaactc tgtataccat
     2341 agtaaatcga gtatccattt ctacatagat atctttttct aggtaatctt tttctattac
     2401 ccaaagagta ccatatacta taaaagcttc gtctgtaact ttggtacatg aataccactc
     2461 atctataatc tcaaagtcta tttgctctga ttctgtttgg tctttttcaa gacctataat
     2521 aaattcttct aatgtttcgt aaaattcatg ttttcctgtt ccaattaatg caaggttgtt
     2581 atctaataag gaaataataa atttaaggtt ttcttcttct ggatttaaaa tataaatatg
     2641 ccaaattttt tttgataaat ctcttaattg tttttgtatt gtattatttt ccatagatta
     2701 cccccttaag atttacagtt tatattttac tgtattataa gttgaactct tattacaata
     2761 ttattataat atagaactaa agaaattaca tcctgtatag gtaaaagtat atcttttaca
     2821 ataaaaatat tacagtaaaa taaccagttt taatatacgt attatatttt taatttataa
     2881 acgaatatta taaatgaaat ttaaattcaa ccctgaagaa ataagctaaa tgtattagta
     2941 tataaatcca tacttgaaca taaataacct ttatgatatg atatcaataa taatattatg
     3001 agaggagtgc cttgatgtcg gagttatata atggtaacca acaattaaat agttggagac
     3061 acatatattc tgtataatca gaaattttaa gtgtattttt aatacattta atttttgtgt
     3121 cttattctgc ctttatagat aactgacata tgacaacaaa tagtatttaa aatatatatt
     3181 actttgttaa attatggtgt agtaggcttt aatctgctac acttttatta attttgtata
     3241 gtattatttt gataactatt ttgaaataga gagcattaat ttttctagtc cacaggagtt
     3301 ttctgttttt gtgctgttaa atataagcac agaaaaaggc agatatatac ctgtggactt
     3361 tttttatata aattatgaaa tatggaggta gttaaaatga gtataattga acttaatgaa
     3421 ctgactaaat cttatagaag atttgagaaa aaggcaggat ttatggggag tgtaaagtct
     3481 ttatttaata ggaaatatac agaaaaaaca gctatatcgt catttgattt aaagatagaa
     3541 gaaggagaat ttgttggatt aataggtcaa aatggagctg gcaaaactac tcttattaag
     3601 atgttaactg gtattattgc accgtcttct ggagaaatat ctgttatggg ttattaccct
     3661 aacaaattag aaaataattt taaaaaacaa tatgctgttg ttatgggcca aaaaagtcaa
     3721 ctattctttg aattgacacc agcagatacg tttcttttat ttaaagaatt atatgatata
     3781 tctaatgaag aatataaaaa aaacttggat tactttatcg aattatttga ggtatctgac
     3841 tatttaaatg ttcaagttag gacgctttct ttaggagaaa gaatgaaaat ggaattaata
     3901 gtagcccttc tacataatcc aaaaatatta tttttggatg aaccaactat aggcttagat
     3961 gctatagccc aaaaacaaat taggtatttc ctaaaagaag taaatgaaac aaaaggaact
     4021 actattatac ttacttctca ttacatggaa gatattaaga gtctctgtag tagaagtatt
     4081 gttgtagaaa atagccgtaa aatatatgat ggttctacag agaaactatt taatcatttt
     4141 caaaaatata aaaaaataac agttagtttt tccaatgaaa ctaacattgt atttccaaaa
     4201 gattgtaaag ttgttgaaca aacaaatcat aagacatcag taattgtgct aaaagaaaga
     4261 ggaaatgaaa cgattaaatg gatttttgat aaatatgatg ttaaagatgt aatgattgaa
     4321 gaagaagata taggtagtat tgtagagaga atatataaga aaggaagtga tgagtattat
     4381 gagcaactat aatgaagcaa gttcaaatta ttcaatgttt tataaattta aacaaagtat
     4441 tagaaggtat tatgaagtag ccaaaattaa ctttaaagtg caacttatct ggagatttga
     4501 tgtggtggtt aatatggtgt ttacgattgt aaaaatttta tttgcttata ttttatggaa
     4561 agcaattttt ggtgaaaata aaatagtctc tggttttaca tttaactcta tgttgtctta
     4621 ctatataata agctcatttt tagcaggtat tgatatgtca aagagtacca gtgaagagat
     4681 atgtaatcgt gtaaaattgg gcacattttc aaagtacatg attattccaa ttaatgttca
     4741 aaattatttt attgctaaaa attttggaat gacatgtttt tattttgtat ttaatatgat
     4801 ttctgcattt gtatggattt ttatatttaa aatagatttt gcctttacat caaatccagt
     4861 tcaaatatta atttcaattt tgatgatttt tctagggttt atttttatga tgaaactcca
     4921 cttctttatt gggattttgt catttaaatt tcttgagatt agttttttta gaatgataat
     4981 tgaaaatatt gttgaattta ttacaggaac aatgatacca cttgtactct taccagaaac
     5041 tatagtaaca gttatgaagg tatttccatt ttattataca acatatttgc catctatgtt
     5101 actaataggt agaaacagca atgaggcaat acaaggatta attattctag cattatgggt
     5161 tttatttttt tctgtagtaa ataaagttat gtataaaaga atgagaacat tatttgatgg
     5221 ggtgggtata tgatgaaaaa aaatttgaaa ataatattta tgttattaaa aatgaaatta
     5281 tcaaagatga tggtatttag atttgatttt tttggagcat tttttgtaga tagttcatta
     5341 tttatattac aacttctaat gtttaatagt atatattctc atgtagactc aataggggga
     5401 tggcaacaag gagagatgtt aatatttata ggaactttct cattaataaa tgcgataaat
     5461 atgacgattt tcttttttgg tatttatgat attccacgaa aaatccaaga aggagaatta
     5521 gactactata ttacaaaacc attagaccca ctatttaggt taacatttga aaatattaat
     5581 attggttcat caccattaat tctagcaagt atacttattg ttttatatgg aatatctaag
     5641 ttaaactcag agataacttt agttatgata ataatatata ttttcatggt gttattgatg
     5701 acattacttt tttatgatgt atgtatcata cttagaacag tttcattttt tgttatatca
     5761 tcttctgcga taatgagatt agaagataat ttattgccaa tgaatatgaa aattcctggt
     5821 gtaatatatg aaggtgtttt taaagtatta ttctatttaa tcttaccata tggaattatg
     5881 tcgacaattc cgactcaaat tttggctggg acaataacaa ttaatggatt aatatactca
     5941 attttaattg tatttttgtt tacattgttt atgtggagat tttggaagtt aggtatgaga
     6001 cattataaaa gtgcaagtag ttaataaatt agtaaaaata gacaatgtat aactatatat
     6061 aaatacgttt catatgacac taaactatgt gaacaagtat ttgcgctaaa ggcactgaaa
     6121 gctataaaat gattaaagta aggatttaaa agtttcttta gtagaaatat aattgtgtaa
     6181 aaaagcaaga ttaattaaaa ctatatccaa ataattagat ataattctta tgcagaaagg
     6241 aggtctgtaa atgtctaatt gtttggaata tattttaaaa ataattaatg tacagaaaaa
     6301 taaacaaaat gagtgatgta tattattcag aaaatcatga acaagataat ctttatgagc
     6361 aagataattt actggcacaa agatttaatt tatttactct attgaagttt gcatttccta
     6421 ctatttttat gatgattttt atggggttgt atacaatcgt agatactata tttgtatcaa
     6481 gatttattaa tacaaatgct ctatcagcaa ttaatattgt atgtccaatt attaatctta
     6541 tagttggaat tggaactatg cttgccactg gaggaagtgc tattgtagca cgtaagatgg
     6601 gaaatggtga tattgaggga gcaaataaag ctttttcttt aatcgtatta tcaggagttg
     6661 taattggggt aataattact ggtataggta ttgtatttat ggattatatc attatatggt
     6721 taggagcaag tgaagtttta attaaatatt gtcgttcata tctttttatt atcttggttt
     6781 ttgcttctgc aaatatacta caagtattat ttcaatgttt atttgtaaca gctggaaagc
     6841 ctcaatttgg attatggcta gtgttaggtg ctgggtgtgc taatataatt cttgactatg
     6901 tgttcattgt tctgttacaa atgggtatta gtggagcagc acttgcaact ggagtaggat
     6961 acctaattcc taccgtttca ggtatggttt tcttttcaaa aaataaagag ttacttaagt
     7021 tttgtatacc cagactagat aaaagaatat tatttgaagc ttgttttaat ggttcatctg
     7081 aaatggtagg tcaactatca acagcagtta ctactttttt atttaatgct atgatgatga
     7141 aattaattgg tgaggatgga gttgcagcta ttacaataat tatttattct caatttcttt
     7201 taacttcgct ttatataggt ttctcaatgg gtgttgcacc aataataagt tataactatg
     7261 gaagtgaaaa taatacacag ttaaatcatg tttttaagat ttgtatatta tttatttcag
     7321 tggcatcttt attggtattt atcttttcta tatcatgttc accaatcatt gtagagattt
     7381 tttctcaaag aggaagccga gtttatgaaa ttgcaaggtc tggattttat atattttctt
     7441 ttagttttat tttttgtggg tataatattt ttacttcgtc tatgtttaca gcattgtcaa
     7501 atggcaaggt ctctgcaata ttatcttttc tacgtacatt tggatttata actataggtt
     7561 tattgatact gccaaatata ttaaatataa atgggatatg gttagcagta ccaagtgctg
     7621 agattttaac tttattttta accatttttt gtatatgtaa gtataagaat agatataaat
     7681 atatgtagat attaaataaa tagtttaggt agatatttta tgttttaata agtaattcaa
     7741 ttagtggtag atatatacta ttgaaaatta aagttataat tgatataaag aattaataaa
     7801 aataacatat aaaagtttaa aagggagttg aacttaaagt ttgattagaa aaagtcaatt
     7861 ttaagacaac tccttttatt ttactattgt attaatctgt atctgtatct gtaagtgtat
     7921 tagtctaata ttataaactt tggatgaaat taattttcta taggttttaa tgtatcatac
     7981 actttatttt aaagaaactt atcttaattt tcatagtgat tgaatttgtc tattagagat
     8041 gttactatta gtaatgtaaa agcaagagta gtgaatataa aaaaacaaga tattgatact
     8101 gcaagattag aatataataa atttttatgg agagatttca atatggatac atattttaat
     8161 aaaaaaatat tacttgttga tgatgaaaaa gatatagtag atttaattga agaagtactc
     8221 ataaatgatg gttttaaaaa cattattaaa gcttataatg gtttagatgc tatttcgctg
     8281 tgtaagatag catgtccaga tgttgtcatt cttgatataa tgttaccaga tatagatggt
     8341 atagaagtgt gtaaaaaaat tcgagagttt tcatactgtt ctattctttt tttatcatca
     8401 aaaaatgatg atattgacaa aatactaggt cttagtagtg ggggagatga ctatataaca
     8461 aagccattca gtccaagaga aattgctttt cgagttaaag cgcaattacg tagacaacag
     8521 tatcaaagca ttgtaccatc agatagtgca gttattaaaa taggagatat tactattgat
     8581 atagaaggaa accgtgtata taaagataga aatgagattg agttaactgg aagggagtat
     8641 catcttttaa gctacatggc tcaaaatgta aataaaatta taggaaaaga aagactctat
     8701 gaacaagtat ggggagtata tagtagtatt tgtgacaata caattatggt gcatatacga
     8761 catataagag aaaaaattga agataacccc tcaaatccta aaatacttat aacggttaaa
     8821 ggactaggat ataaacttgt gaatagaatc gattagaata gattttaata ttaatgaaga
     8881 tgtctcaagt tgaattttta gttaatgagg cattctttta tgtgtgaagt caaatgtatt
     8941 ttcgttatct caacaatgaa gaagaggtct atctctattt tgagatagcc tcattaagtt
     9001 tatatcctgg taatgtactt tatgttctaa taaaattttt ttagtttatt tgcagtgaaa
     9061 cattaactag aacattaaaa tttgcattga aatatcaatt aaataatatt gtatattgtg
     9121 tataaactta tactaattgt ttttctatat taaacacttt atcaatccaa cctttataaa
     9181 atttttcttc atgggaaact aggattatac ttcctctaaa ctgtttcaaa gcattttgta
     9241 aagcatcctt tgtctctgca tcaaagtgat tggttggttc atccagaata agaaaattac
     9301 aaggagataa catcatacaa cataatttaa ccttagattg ttcaccacca cttaaagtag
     9361 atacactcct tgaaacatgc tcttccttca caccacatct ggctagatga cgtctaatct
     9421 cttttgtatt taattttgga tatttatctg ctactatttg aagtggggtt ttatcaggat
     9481 tttcccattt taaatcttgc tcataataac caattttaac ttgttctgaa aactggaaat
     9541 ctccagatat acgaggaata tctttgacta atgtctttaa caaggtagat ttaccgattc
     9601 cattaaaacc agttataact attttttgac ctccatcaac agaaaaattt aattttggaa
     9661 gtaaagaata gtagtatcca acttcaagat ttgttatagt aagagcattt tgagccgata
     9721 tttcaatttc tgaaaattga atatttggtt ttcctgtaaa actaggagga gctattcttt
     9781 ctattttatc caattgtttt tgtcttcctc ttgcaatttt tgaatttact ccagctttat
     9841 ttttacgtat aaatgtctct tctttttcta ttttcttttg ttgtgcttga taccttctaa
     9901 tataatcttc tcttaaatgt gatttttgtt taaggaactc tgaatatttt ccatgatatt
     9961 ttttaatcat accaaattct atatcaagaa tacctgtaga aattttttct aaaaaatcaa
    10021 aatcgtggga aactacaata aatgccccat taaatgtatt taaataattt gagagccatt
    10081 caacatgttc tttatctaaa aagtttgttg gttcatctaa taatagaaca tcatggttag
    10141 aaagtagcaa ttttgctaaa attacctttg ccctttgccc accactaagc tcttcaacaa
    10201 cacgattcat tcctattgaa tctaatccaa gaccatttgc tattttattt atttcattat
    10261 ctatagaata gaaattactt gctattagtt gttcctgata atctgatgct ttgattagat
    10321 attgctcatt ttcagatata gcactttttt gatagagtaa attcatttcc ttttctattt
    10381 tataaagttc ttcaaatgca gtgtgtaaat agagtgatat tgttgtatct ctattgattt
    10441 ctgcatattg gtctaaatgg cctatattga tattaggttg ccacttgatt tctcctgaat
    10501 ctggaacaac ttctcccaat aaaattttaa ttaatgtact tttacctgta ccattttggc
    10561 caactacacc tatatgttca cctttgaata aatcaaagtt agctttctca tacagtgatt
    10621 tatccataaa acattgtgat aaattagtta cttttaataa actcataatt caaactcctt
    10681 ttcttttatt aaaaaattta tggaaataaa aaaagcagag gaatggcatt gtaaaatgcc
    10741 gacctctgct tttggaaaaa caaaaggtca tagacaagca ttgttgtcta tgaccagaaa
    10801 atctatacta tctatcggca aataggtaat tttaaataag catagccaaa aaactatact
    10861 aaacctatat ttgccgatga agatagaaag ctttaagctt tcagattaac ctctacacaa
    10921 cgcttcatcg tatacatttg tgtagagtta agttttctaa caagtttaac taattctata
    10981 taaatcataa ttattcacat cctaatttta gtattaattc tagtttatct atatttaaat
    11041 tgtacttttg tactttgtgg attatcttat ttatttaaaa tatgttttat taattatagc
    11101 ttaagttatg ttatcataat acttggttat agtcaataag a
//
LOCUS       DAEPOZ010000127        11115 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.127, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000127 DAEPOZ010000000
VERSION     DAEPOZ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11115
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.127"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            333..1631
                     /locus_tag="KR440_002420"
     CDS             333..1631
                     /locus_tag="KR440_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HBH0699458.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSTERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKGIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLEALEIFEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSGGIFSSGGTVEGNSKGQFESWNRTIEFYKKLLSSDLNN"
     gene            1821..3212
                     /locus_tag="KR440_002421"
     CDS             1821..3212
                     /locus_tag="KR440_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HBH0699459.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            3389..3976
                     /locus_tag="KR440_002422"
     CDS             3389..3976
                     /locus_tag="KR440_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0699460.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSKSVSPYLLLPLSYIEFAQEETYLFKL
                     LFINDLDLMMKEANDFYKEIDNEKKARLFSESIGIELECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKPDWNLSI"
     gene            4009..4800
                     /locus_tag="KR440_002423"
     CDS             4009..4800
                     /locus_tag="KR440_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH0699461.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(4981..7398)
                     /locus_tag="KR440_002424"
     CDS             complement(4981..7398)
                     /locus_tag="KR440_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0699462.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKDKNLSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLKKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKTISLQGNILSIIFIPLGAI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKQAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     SYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSSI
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLIRNIEKKSL
                     TERLRSQ"
     gene            complement(7411..8076)
                     /locus_tag="KR440_002425"
     CDS             complement(7411..8076)
                     /locus_tag="KR440_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0699463.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMTDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDMPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGSLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            complement(8158..9189)
                     /locus_tag="KR440_002426"
     CDS             complement(8158..9189)
                     /locus_tag="KR440_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0699464.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFNISIYLITDDLKLIALLL
                     IYTIVLCGFIALIIFLLHKKIVIFCSKICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDLSHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTNSNGKIRVATECWVMYTKIDII
                     DNGKGISESHQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            complement(9179..9865)
                     /locus_tag="KR440_002427"
     CDS             complement(9179..9865)
                     /locus_tag="KR440_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0699465.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINLFKQKVKAFLNHLTTKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            complement(10023..11087)
                     /locus_tag="KR440_002428"
     CDS             complement(10023..11087)
                     /locus_tag="KR440_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HBH0699466.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
BASE COUNT         3972 a         1465 c         1573 g         4105 t
ORIGIN      
        1 caattatata aatttaaata aaaaaatatc cttattaata acaaatagtt tatattaaaa
       61 accattctta aaatgagtgg ttttttattt tgaatagata tattttaaac ctaatagcat
      121 tcctatcttt aattagccaa aaatcaactg gatttcatat agactacata attttaaatt
      181 actaaaaaag acaatatatt gacaaaaatg aaatatgttg ataaaatgaa atagtcaata
      241 tggtgtctta atttaaaaat ttatttatgt tgtattagat aaagttgttt tatatataaa
      301 gcataaataa ttattataag gagaaataga ttatgaaagt aaatgtatac aatgtagaat
      361 catttgtgga tgaaagactt aagattatgg tttcagactt aattccaaat actaaactta
      421 aaataagtgt gaaaatggaa tttccatggt gcaagggaga agaattttca tcttatggag
      481 tgttttgttc aaatgaaaaa ggagaagtag atttagattt agttgaacct atagaaggta
      541 cttataaggc tgataattgt atggggttaa tttattcgct ggaaaaatct aaaactgaag
      601 gtaaaaattt tgcagaaaat atatctattg ataaaccaat aattatgaat atgatatttg
      661 aaacttctac tgaaagaaaa gaagttgaat taaagagaat atttaaatca gaagatatta
      721 ccacaaaata catatctgat gagtttatag gaaaattatt ttataaacaa aattctaata
      781 ataaaacaat tttaatgcta ggtggttctg atggaaattt agacgctcta gaccttttag
      841 cagctccact tgcatctaga ggattcaatg tactaacagt tgcatacttt gctttagaag
      901 ggttaccaga taaattagaa gaggttccat tagaatattt tgaaaaagta tttgagtgga
      961 ttagtgaaaa tgaaattacc agtactaaag aaatatttgt acatgggaca tcaaagggtg
     1021 gagaattggc ctttttactt gcttcaagat atgaacaaat taaaaaggtt gtagtatcac
     1081 aacctcacat ttattgtttc caagctctta atggattgat gagtggaaat gatacttcat
     1141 catggtcata taaaggagag ccatttccat atatcaaagt agacaatgat atattctttg
     1201 aagaacagaa aaaaaatata tcaaaaggta ttccatttgg atttaatagt acttataaga
     1261 aaagtcttaa aagagcagag aatagaaaag aggctagaat aaaaatagaa aattccaaag
     1321 cagatatact tatgattgct ggaaaagaag ataacatatg gaatagttat gaagcttgtt
     1381 tagaggcatt agaaatcttt gaagaaaata attatccata taacgtagaa ttattaacat
     1441 acaataatat gggtcatccg ttaccaattc catatattat gcctctaaag gagactttat
     1501 gtatgagtat gagtggaggg attttttctt caggtggaac tgtagaagga aattcaaaag
     1561 gacagtttga atcatggaat agaactattg aattttataa aaaactttta tcaagtgatt
     1621 taaataacta atattaagtg atttaagtaa ctaaaaatga agcggttttt ctatatatgc
     1681 acaaaatcta tcaattttat agatttaatt tattgtttat ataatttgca tgttatatac
     1741 taattttaat gacattgaca atttataaac aattataaag ttgtaaactt ataagagtta
     1801 ttgaaaggtg gagtgaagtt atggaaagac tcgctagaag ggaacaacct catataaaat
     1861 ttggaatgtt taagataaga attccattta tacattacag atttgagaaa ccagaagcta
     1921 ttcaaggtat aatatcaagt atgacaagtt taggcacaat tggccttagt acacaaattt
     1981 tgggattacc ctatgaagtg gcatggagta tggctattat taactcaata ttatactgtt
     2041 tgcatgtttt tatgggagac ccagttgtac ctggctggat tacagcttct ataactctta
     2101 caactgcata tttgttaaag ttcagcatgg gcattgaaag aatacaagca ttaacagctt
     2161 tacaaataga tttaggaata atatttattt taatgggtat aactggtgta gctggaaagt
     2221 tagttagtaa gataccaaat tctattaaag gtggaatact aatgggtgtc ggtatatcga
     2281 ctataatatc agagtttaat cctaaaggga gatttgattc ttatcctata tctataacag
     2341 taggaatttt ggttgcttgt tttgtaatgt tttctgaaag atttgatact ctaaaagtta
     2401 agaataaatt tttatttcat ttaggagaat atggtgtagt atcagctatt ttagtgagtt
     2461 taatagtagg tatatttagt aaagaaatag aaattcctag atttagtttt gacaatttag
     2521 tttacatagt agattttaaa aatctgataa atacagtatc tccttttgga ataggttttc
     2581 caagtgtaat gttatttata caagggatac caatggcttt tatgatatac ataattgcct
     2641 ttggtgattt tataacaggt gaaaacttag tactttctga aagtgaaaat agaaaagatg
     2701 aatatataga tttcaattca aataggtcta atgttataag tgggataaga aatattttta
     2761 tggcaatctt ttctccatat ataccaatgt gcggtccatt agctgcgaca cttacaggaa
     2821 gtgttgctca aaggtacaag gttggaaagg aagccatgca gtctgttttt agtggcatgg
     2881 gtacattagt atgggtttca gcaattttta tctgttttta tccaattgcg cagattgcta
     2941 cacctcttat accattagca ttgtcagtaa cacttttagt tcaaggatat ttatgtacaa
     3001 agctatcaat ggagctatgt aatacaggtg ttgagaaggg attgattgga cttatgggag
     3061 gtgtaattgc tgctaaaggc ggaacatggg gacttgctgt aggttttata ctgtatttca
     3121 ttttaattga ttctaaaaaa agaaaagaaa aagatattaa ttcaatagaa gattgtgtat
     3181 atgaaatatt agacaaaaag aagagtattt aaaggaaaat atgagttgct gttatctatt
     3241 aatagattaa ttgcaactca tgttttattt ataagtttta ggaattgcaa aaaaatgcaa
     3301 agctttttct tgacataatt tcttgatatg ttactatagt tatataacat atgtgtttta
     3361 taaaaattta atggagaggt gaacatatat gccacctaaa gtaaagataa caaaagaaat
     3421 gattttgaat acagtcttgg atattacaag agaagtggga tttgaagctg taaatgcgag
     3481 gagtattgca agtaaattac agtgttccac tcgaccaatt tttacttgtt acgaaaacat
     3541 gaatgaatta aaaaatgaat ttcttgcttt tgcatatgaa tattataatc agtatgttgt
     3601 taattatcgt aattctaaaa gtgtaagccc ttatttactg cttccgcttt catacattga
     3661 atttgctcaa gaagaaacgt atttatttaa gttgttattt ataaatgatt tagatttgat
     3721 gatgaaagag gcgaatgatt tttataaaga aattgacaat gaaaaaaagg caagactttt
     3781 ttcggaaagc attggaatag agttggaatg tgcaaaagta atatttttag atttatttct
     3841 ttatactcat ggcatagctg tcttaacagc aacaaaaaaa ttaacattag atagatatag
     3901 tgctgaaaaa atgttgaaaa atatattaac agcttttata agacaagaaa aaccagattg
     3961 gaatttgtct atttgatgtt tattaactaa tatagaagag gtgagattat ggaaacaaaa
     4021 caatacttaa ttgactttta taatacttat gatgaagata gtcgacttgc attaaaacat
     4081 ggaatggtgg aatttctcac tactatgcat tacattgata agtacataaa atcaggtgat
     4141 tgtgtgcttg aaattggcgc tgcaactggt cgttattctc acacactggc acgtcaggga
     4201 tatgatgttg acgcagtgga attagtcgaa cataatatag aggttttcca taaaaatact
     4261 caatcaaatg aaaatataag cattactcag ggtaatgcta tggatttatc tgttttccca
     4321 gataataaat atgatattac gttactgtta ggaccactat atcatcttta taacaaagaa
     4381 gataaacaac aagctttaca tgaggcaatt cgtgtaacaa aacctggtgg tgtggttttt
     4441 gctgcatacg ttatatctga tggctgtctc attgacgagg ggttccatag aggaaatatt
     4501 aatgtatctg aatatattga aaaaggtttg attgatccac agacatttgc tgctaagtct
     4561 gaaccaaagg atttgtttga gcttgtacgt aaagaaaaca ttgatgattt gatgtctgca
     4621 tttaatgtaa ctagactgca ttatgtcgca tcagatggtt tggctttgta tatgcgtgaa
     4681 gcagtagact ctatggatga tgatgcattt gcgttatatt taaaatatca ccttgctact
     4741 tgtgaacgtg aagatttagt aggagttaca agtcatgcaa ttgacatatt tagaaaataa
     4801 ggaatatgct tgtttatgtc tctggtgatg ttgcttggag atataaggaa gtgtatctgt
     4861 ataaaataaa aacaagtaga gggacagaaa attatgtaaa gtcaaaattt tctatccttt
     4921 tagcttttaa aaaattatgt taaaacttgt ataaaaaatg gttatttttt atatacattc
     4981 ttactgagaa cgtaagcgtt ctgtaagtga ttttttttct atatttctta taagtaattc
     5041 agtcactaaa acttgtatac caattaatac gaatgaaaaa attaaaatag taaatataga
     5101 aaatttaaaa ttaataaagt ctaatctaat tacatctttt ataaaagtgc aaagtagatt
     5161 tgaaccaaaa taacctagta tacttgaagt acatatagct ttgatactta tgtttagacc
     5221 ttctctataa agcatatttc gcagttgctt tcttgtcatt ccaacggctt gaagtaaggc
     5281 aaactctttt tttctaatta aaacgctgtt tattagggta ttggtcatat ttagtatacc
     5341 aaagcaagaa attaaaccaa caaaaatgta tgaagctata attatatatt gaaatgctct
     5401 agtaatcatt ctataatcat cttcatatga ataaagcatt aaatttgaat ttttctgaat
     5461 tatacttaac aacttttctt caacttcttt agaatgcttt ttatctatta aaatagaaaa
     5521 atctaaattt gtattatatg gtgttagaga ttctaaagtt ttgtctgaag caaaaaacat
     5581 acctcctgta ttggaattgt caatgattcc tataattttc attttctttt taataatatc
     5641 atttccacta aagatattaa atgtaactgt atctccaact tttaaattta atccataaga
     5701 ttttgttcta tatttattta taattaaact atctgaatct aaatgtgtat agtctatttt
     5761 tccactgaca atatattctt caagtgattt tgaaagttca ggcgtaatac ctttaattag
     5821 agtaataaat tgtttttcgt ctctatcttt gatgtaaggt tctataatat tacttttaat
     5881 tgatttggat tcaattaatt tttttactcc atgaatagat ttaatttcat tcttaagaga
     5941 atctgttagt ggattatttt gttggatttc tgtaatatgc tcatataagt tatcactctt
     6001 aatgctaatt atataatctt cgttatagac aaattgttga gatagttgtt ttttgaaatc
     6061 tattgaatta gcaagattag cgattgaaat aaataaaact ccactaatgc ttagcgatat
     6121 taatgcaatc aaatttttct ttctatttga ctcgatattc tttaatgcta gattttttat
     6181 atttatataa ctaaaatttt ttgcttgttt tctaaattta gtttctgtat attgcatggc
     6241 ttcaatagca gatattttag aaataattct agctggttta aataaagaaa ttctaacagt
     6301 taaaaacatt accagtgata aaattaaaat tataaataaa tcagctatca tttgccatgc
     6361 taatggttga attatgtaag caattataca agaagctata gcccctaatg gaataaaaat
     6421 aattgacaaa atatttcctt gtagagaaat agtctttttt aactgaaaag aagtcatacc
     6481 taacgatttc aacttagcat acatctgaac tgaattaaca actgaaatat aaaaaatact
     6541 atatataaca aaaacgctag aaaaaagaac aactattcca actactaaaa ctgtaataat
     6601 ctgctcacca tcaattaaat ttgattcaat atagttatca tttaagtata tattatattt
     6661 cttaatacct aaagataaac caattttctt taactgtgtc ttaattgaag ctgaatcttt
     6721 ctctgtattt tcttctgata gtttaactaa tacatctaca gtatcaggtc ttgactcgtc
     6781 aaaagtatta cttttatttt gtaaaggtgt agaagctata ttcttaaaat aatcatttga
     6841 aacgactgta tagtattgct tggcattatc ttgataataa ttttctagaa atccaacaat
     6901 aataaaatca cattgaactt ctttattcgt aatatcatca taataattta atttaatgct
     6961 atctccaata tctttatcta aaccaaattc ctgtaaatat ttttctgaaa tagcaatttc
     7021 atttttattt gtaggtagtt tgcctttcaa tggttgaaaa ttcataagtt taattgctgt
     7081 atcatctgca taaacaatcg acataataca gtctgtcttt ttattacttc caattaattt
     7141 gtaggttcct acgccactaa ggtttttatc tttatatagt atattttttg ctttttcatc
     7201 tacagattta tatatagcat gatatggaga tgcattaact aaatctacag aagcattata
     7261 tgaaactaag gtcactgtta ataataaaca tgtagaaatc attataatga tacctgaaag
     7321 atatgtttta agtttattaa tttttaaatc atttaaagct atttttctta taaatgtgcg
     7381 attattattt ctaaacatat actacacctc ctagttaaca atttttccat cttcaatacg
     7441 aataattcta tcagttagtt gtgcaatttc attattgtgt gtaatcatta caattgtttg
     7501 gttgaatttt ttacttgtca ctttaagtaa actcagtaca tctgaactag ttctactatc
     7561 aaggctacct gtgggttcat cagctagtat aatagctggt tttgttacaa gtgctctagc
     7621 tatagcaaca cgttgctgtt gaccaccaga aagattactt ggcatatcat ataatttttc
     7681 ttcaagagaa agcatattta taatttcatt tataaaatcc ttatctatgg tctctccatc
     7741 caatcctatt ggtaagataa tattttcata tacattaagt aaaggaacaa gattataatt
     7801 ttgaaatata aaacctatat ttctgcgtct aaagattgta agctgttcat cagtcatagt
     7861 agataattcc ttacccttta taataacctt tccagatgta ggattatcta atcctcccat
     7921 catatttaat aatgtggatt tgccactacc agatgtacct acaatagaaa taaattcgcc
     7981 tttttcaata gatagagtta caccatcaag agcttttgta atgtttgaac tttgtccata
     8041 atatttcttt aaattaatta cttgtaaaat actcataata atacctcctt aacatcttgt
     8101 aaatacagta taaaatcaaa atgttgcaga atcattacaa ataaaatatt taaaaattta
     8161 tttattaggt aagaagatag aaaaagtcga cccttttcca atatctgatg taactttaat
     8221 atagccacct tgaagagata taatttttct tgaaagatat agacctattc caataccttc
     8281 tatgtcatgt acatcttctt cacgataaaa tcttttaaag atttctgctt gatgactttc
     8341 tgatattcct tttccattat caataatatc tatttttgta tacataaccc aacattctgt
     8401 agctacacgt atcttaccat tagaatttgt gtattttaca gcattatcta atatattaaa
     8461 aatagcttca gaagtccatt tcttgtcatg aattatatat aaatttgggt cacaatcaac
     8521 tgtaacttca atattctttt tttcagcatc aaacaaaatt ccactaagtg ctgtagcaat
     8581 tgtttcataa attgaatttt tctttttaga tagtgtgatt atacctgtct ctagtctgga
     8641 ggtttttacc atagattgca ttaaaaaatc taatttatct aattggtttt ccatatccat
     8701 taaaaaatcc ttttgaattt ctgttttaaa tgtttgattt agtagagtag tattaatcat
     8761 ttttaaattt gcaataggag ttttaatttg atgagataga tcagaaacta gttcttgtaa
     8821 atcagctttc tctatagcaa tactattgcg attttcttga ataacttcat acattctaat
     8881 taatctgtga ttgatacgtg ataaaatagt ttcttcttct aattcaattt caatattgtc
     8941 ttcactattc atcatactat ctaaagtgtg acatatttta gaacaaaata taactatttt
     9001 tttatgaagt aaaaatatta ttaatgctat aaatccacaa agtactattg tatatattaa
     9061 taatagagca attagtttta aatcatcagt tattagataa attgaaatat taaataaaat
     9121 agctgtaaaa attataccaa cagcaattat taaataaata gaactaaaag aaaatttttt
     9181 aaaattcatt tctttcacct aaccacatat atcccatacc atataccgtt ttgatatact
     9241 taagattgtc tttttcaatt ttgcttctaa tcctacttat aacagatgtt aaagtatgtt
     9301 catctacaaa gttagcatct atatcccaga gtttttctaa aagtactttc cttgttaaaa
     9361 tacttttagg attttcagta agcagtttta aagtacgata ttctaatggt gtaaatataa
     9421 tttgatttcc atttatattg gcagataact cagaaaagtt aatttcaaga tatccatccc
     9481 tatagtaatc ttgttttgtt tttgttgtaa gatgatttaa aaaagcttta actttctgtt
     9541 taaataaatt gatattaaat ggttttgtta tataatccaa agctccaagt tcatagcctt
     9601 taatcatatc acattccata tcattagcag ttaaaaaaat tattggtata ttgtaatttt
     9661 cctttatttc attacataat tcaaacccac ttttatctgg taaattaata tccaaaatta
     9721 ttaaatcaaa ttcacattca aaaatagact ttagtgcaga tttagctgta aattttgaag
     9781 taattatata tccatcttca attaggttat atgatagagt tttatttaat atagaatcat
     9841 cttctactac taatattttt tccatacagt tactcctttt agtttaatat tttataatgt
     9901 caattataga ctaaaaacat tgctgaattg ttataaacga gaaaaagtat ttaaaaattt
     9961 atatatttaa taaagaacct gtcctatgat aaattctagt acagattctt tattaaataa
    10021 aattatttca accaagatgg atttccaggt ataaatattc ttttaccaaa ttttgaagta
    10081 tactcatcta aagctttttg aataagctca ttattaccct taaatgaagt agtattcttt
    10141 atagtttcga gattttggct aaatgggcaa gcagatatac acataccaca atctgtacct
    10201 aaatatctcc atttaatata gcatgtctca tgttcaatta cccaattata ttttccatca
    10261 tcaccaattt taatatcatt agaaagcgaa tgagaagggc agttaaaaga gcatttttta
    10321 cagaccttac aaaaatcttc tagtccaaaa tcaacatatt catcttctaa aagaggaata
    10381 tctgtagtaa caacacctaa tctaagtcta gagccataat ctttatttgt aagtatagca
    10441 tttcttccta tatctcctaa acctgcatca cgagctataa aaacaggcat tactaaataa
    10501 ttagcatcca tatgatttct agcatcatat cctaaatttc ttatatagta acttaaaatc
    10561 atgccgacga tagatgcatc cacatagcac tttgatgttt ctataacttc acatatcatt
    10621 ggagctctat ttatcatttc tttatccatc tcacatccaa atacaattgc atacttatga
    10681 gttagattaa tttcttcacc gtaattttct tcatgtctac ccctatttgt ataaatatga
    10741 tagtctttta atttagttat accaactaca gaagctccat actgctttgc aagaccctta
    10801 attttttttg tcattgactt tgcatctaca tctactttag tatcagaaac ttttccttcg
    10861 cataaagatt tgatatcaga taaaaaatcg aatgcactac ttgccattgg agagtttaac
    10921 tcattgtaag tcattgttcc ttcactacat aaatttggtc tatttcttat actatcatca
    10981 atttcttttt tgtcaggatt tttcttataa taatcattat aagcagtact atcttttttg
    11041 taatttgctc ttgcaaacat agtatttctc tcatcaaccc ttttcaattc tattccccct
    11101 agtaaaaata tattt
//
LOCUS       DAEPOZ010000128        10790 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.128, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000128 DAEPOZ010000000
VERSION     DAEPOZ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10790
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.128"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..471)
                     /locus_tag="KR440_002429"
     CDS             complement(<1..471)
                     /locus_tag="KR440_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0699467.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLN"
     gene            790..1929
                     /locus_tag="KR440_002430"
     CDS             790..1929
                     /locus_tag="KR440_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBH0699468.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVAGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
     gene            1981..3390
                     /locus_tag="KR440_002431"
     CDS             1981..3390
                     /locus_tag="KR440_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH0699469.1"
                     /translation="MKKKIGFWDLVFMNISALFGIRWIAKSTASSFGLGLGSIPVWLL
                     FAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLNWTSKIFWYSSFL
                     TFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGKFFTNTGALGSTI
                     PAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAIIFALSGAETTANF
                     ITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDKIAASTGILDALAKVAQD
                     LGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQLTVTNKYNIPSN
                     AVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFTSYIRLRKTRPNE
                     ERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYEIEMIGGGLFVII
                     TGLMLWKNYEKKISNNSGR"
     gene            3574..4869
                     /locus_tag="KR440_002432"
     CDS             3574..4869
                     /locus_tag="KR440_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0699470.1"
                     /translation="MSVEVNKPSIWKNFKVLIILFVAGAFIYALPYLKNYYYDTFAQA
                     FNLTNTQMGSLGSIYGLLAMVSYLFGGFLADRISARKLLSGSLIITGISGLVLTLYPP
                     YIVVFLIHALWGITTILTFWPALVKAVRMLASENEQGKAFGFMESGRGITNAIHLSLV
                     LILFGYISSKVSDKSGLTAIIVLYSAINVIVGVLVFFKLKDNEKEVSAKFDKEAFFAV
                     IKMPHTWIITIIMFCSYSANMSFYYFTPYSTEAFGATVVFASAISILAQYCRPVASAA
                     SGLLGDKVGSSKIISYGFILMIAGLLGVIFTPTKSSMIYILLIGCVAIYVSMYAIQGL
                     HYSLLEEGNYSLSISGTAIGIVATLGYLPEVLCPLAAGKILDAFPGVTGYRYYFIVLT
                     IVAIIGLVFTFIWMNMTKERRKELIEMNNKSNENTTKVS"
     gene            5041..6516
                     /gene="spoIVA"
                     /locus_tag="KR440_002433"
     CDS             5041..6516
                     /gene="spoIVA"
                     /locus_tag="KR440_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HBH0699471.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            6700..8640
                     /locus_tag="KR440_002434"
     CDS             6700..8640
                     /locus_tag="KR440_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699472.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCNMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLRDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAKDSGYNIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNQLDIDVEWDFTDYYEKLEKGENFDITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIEKKTIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNSDEKQPNTPKDD
                     VESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNPITENDDNYDLSSKSVFGTNEH
                     SFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIFLFCILTPIMI
                     GSILIVNPDTRKSIIKFIKLKK"
     gene            8930..9199
                     /locus_tag="KR440_002435"
     CDS             8930..9199
                     /locus_tag="KR440_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBH0699473.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
     gene            9225..10580
                     /locus_tag="KR440_002436"
     CDS             9225..10580
                     /locus_tag="KR440_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HBH0699474.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPISTNSSELFTQRGGRIPAPIHSFK
                     N"
BASE COUNT         4190 a         1174 c         1974 g         3452 t
ORIGIN      
        1 gtttaatagt atcttatttc tttcctttat actttctcct gtaatcatta aagatatgtc
       61 actatcatga atacctaaat cttctggaaa catatcataa tattgttttg ttatatagct
      121 aagttcatca ctagacaagt taaaaaatac ctgataaaaa gcttgagcat tgtcaaaaga
      181 atgcttacaa aataatttcc acataattat atatttctta tattcatcat tgacattttc
      241 tatacatctt gaaatattca agttataatc tttaaaatat tttatagatg caaatgatat
      301 taaatggtct aaatttttaa agtatttata tagagttgaa ctattatatc cagccatatc
      361 tgcaacctta cgaagtgtaa tattttctat accttcgttt tccataatat tttgagcagc
      421 ttctatgaaa tacatcaata tccttttctt ttgtaattct ttatttgtca ttttattcac
      481 ctacaaataa tcaatatagt ttatataatc gtgattttat cattaaactt accataaatc
      541 aagaataata ttccaaaata caatgtatac tcgttatatt ttagataatt taaaatcact
      601 agaatataaa aaaattttaa aaataaaatc gtgattgaca aaataatcat gattatgata
      661 aaataatcac aaataaaatt aatttacatg caaatacgac aagtactaaa aatattaaga
      721 attttaaaat aatatattta tccttgttgt attgaataaa ttatctgaat attaagaaag
      781 aggtgcatta tgtcaaataa tatgagacta gaaagattga gaaagtacat gaatgaaaaa
      841 agcattgaaa ttgctttaat atttgaacca gacaatcaat tttatattag tggatttaaa
      901 gcaataactt attctagacc aattgtaaca gtagtaactc aagataaaat agaattaata
      961 gtacctggat tagaagaatt acatgctaaa gaagttgcaa aagttgataa tgtatatgtg
     1021 tattatgaaa taccagaaat gaaagaacat ggaatatcac ataaacatta cttagatata
     1081 attttaaata aataccctaa aggaacaact gttggaatag aaaaagatat agtatctgca
     1141 agtttttcag agtatataac tgataagtca tttgctatca aagatgttgg aagtaaaata
     1201 tttgaaatga gatatgtaaa agatgcaaaa gaaatagaat ttttgaagat agcaggttac
     1261 ctatctgata taggaataaa agggtcttta gaaaatgtta gagttgggat gagtgaattg
     1321 gagtttgacg ttgcggggga taatgcttta ctaaaatatg tttctgaaaa ttatcctgat
     1381 acatatatag gatttgcaaa ctggacttgt tcagggattg atagaacagc acaacctcat
     1441 ttagattcca atactaggat acttcaaaga ggagatatag taatacatag tagacaggtt
     1501 tggtatgaaa actatagagc agaaaatgaa agaactttta ttataggtaa accaacagaa
     1561 agacaaaaag aagtgtttaa gattgcagta gaggctcaac aagctggatt agatacaata
     1621 aaagctggta taccagcaag aatggtggat gaagcagcaa gagctgttgt tgcaaagtat
     1681 ggtctagagt tatattcaaa tcatagaatt ggtcatggtt taggtttatc agaacatgaa
     1741 gagccatatt taagatttga caatgaatta atattagaag aaggaatggt attttctatg
     1801 gagccaggga tatatatacc tggagttgga ggatttagac actcagatac agcaatagta
     1861 ggtaagaatg gtgctacaat tataactaat tatccaagaa gtgttgaaga acttatactt
     1921 gatatttaaa tcaatacatt gtgtatgagt ttgaataaat ttgaaattgg aggaattaca
     1981 gtgaaaaaga aaattggatt ttgggattta gtatttatga atatatcagc actatttggg
     2041 attagatgga tagctaaatc tactgcctct agttttggat tgggtcttgg ttctatacca
     2101 gtttggttat tgtttgcttt tatattcttt gttcctgcat cacttatatg tgcagagttg
     2161 gctgcaacat atccaaaaga tggtggactt ggagaatggg ttaaacaagc ctatggagag
     2221 aagtggggat ttatggtgtc atggctaaat tggacatcaa agatattttg gtattcttct
     2281 ttcttgacat ttttagctat aaatattgca tatatgcttg gaaatccaga tttatcaaac
     2341 aataagatgt ttattttagc actttcactc ataatatttt ggctgttgtc cttagtaagt
     2401 actcgtggga tggcatttgg aaaatttttt acgaatacag gagcattagg ttctacaatt
     2461 cctgctatat tacttatagt gatggcattt atgtctgtag ttattctcaa aaaagctcca
     2521 tcagcatcga tatatacaat ccaaaatata ataccaaaaa tagatgccaa ttcattagtt
     2581 tctatatctg ctataatatt tgcattatca ggagctgaaa cgactgctaa tttcataacc
     2641 gaaatggata atgctaagaa aaactttcct aaagctatat taactgtggc tgttttaata
     2701 ggaggaatct atatcttagg ttcagtagca ataacaatga tacttcctcc agataaaatt
     2761 gcagcgtcta cagggatact agatgcatta gctaaagttg cacaagattt aggtatagga
     2821 agttggttta taagaatagt agcatttgga ataactttat cagtactagg agcattaata
     2881 ctttatatag caggtccagt aaagatgctt tttggaaatg taagagaagg tgtatttcct
     2941 aagcaattga ctgttacaaa taaatacaat ataccatcaa atgcagtaat agttcaggct
     3001 ataatagtga gtctacttct tgtaggaaca aacttattac catcagtaga taatatatac
     3061 aatgttcttg ttactatgac agcattaaca gcattatttc catatgtctt gctttttaca
     3121 tcttatatta gacttagaaa gacaagacca aatgaagaaa gaccatattc aattgcaaaa
     3181 agtgattcag tttgtatcaa tattgccaga atggtacttg tagtaactgt agttggcata
     3241 cttttctcaa cggcaccagt tatggagact ttaaaagata atataatcta tgagattgaa
     3301 atgataggag gaggtctttt tgttataata acaggtttaa tgctatggaa gaactatgaa
     3361 aaaaagatat ctaataactc tggacgttag tataaataaa aatataatta ttatggtgag
     3421 ctaaaagagt ttacttttag ctcataactc accataaatt attgatagat taacctaata
     3481 tattaataaa tattccgaaa tattgaataa acaataaaat aagcaataat taattatcat
     3541 gaaaatttac aggtatttac ataaggagga agtatgtcag ttgaagtaaa taaaccaagc
     3601 atttggaaaa atttcaaagt cttgataata ctctttgtag caggagcatt tatatatgct
     3661 ttaccatatt taaagaatta ttattatgat acttttgcac aagcatttaa tcttacaaat
     3721 acacaaatgg gaagcttagg aagcatttat ggtctacttg caatggtatc ataccttttt
     3781 ggtggttttt tagcagaccg tatttcagcc agaaaattgc tttcaggttc tttaattatt
     3841 acaggaatat ctggtcttgt attaacactt tatcctccat atattgttgt gtttttaatc
     3901 catgccttat ggggaataac aacaatactt actttttggc cagctcttgt aaaagctgtt
     3961 agaatgttag catctgaaaa tgaacaagga aaagcttttg gatttatgga aagtggtcgt
     4021 ggaattacca atgcaattca tctatctcta gtattgatac tatttgggta tatttcatca
     4081 aaagtaagtg ataaatctgg tcttacagct attatagtat tatactcggc aataaatgta
     4141 atagttggag tattagtatt tttcaaatta aaggataatg aaaaagaggt atcagcaaaa
     4201 tttgataaag aagccttttt tgcagtaata aaaatgcctc atacatggat aataacaata
     4261 ataatgtttt gttcatattc agctaatatg tcattttatt attttacgcc ttattcaact
     4321 gaagcatttg gagctactgt ggtgtttgca tctgcaatct caatattagc tcagtattgt
     4381 agaccagtag catcagctgc ttctggatta cttggagata aagtaggttc atcaaagatt
     4441 atatcttatg gatttatact tatgattgca ggtcttttag gtgtaatatt tacaccaaca
     4501 aaatcatcta tgatttatat actattgatt ggatgtgttg caatatatgt atctatgtat
     4561 gcaattcaag gtttacacta ttcgttactt gaagaaggta attattcatt atcaatatca
     4621 ggaactgcta taggaatagt tgctacatta ggatatcttc cagaagtact ttgtccttta
     4681 gcagcaggga agatattgga tgcatttccg ggtgtaactg gatataggta ctacttcata
     4741 gttcttacta tagtagccat aattggattg gtgtttacat ttatatggat gaacatgaca
     4801 aaagaaagac gtaaagaact tattgagatg aataataagt caaatgaaaa tactactaaa
     4861 gtcagttaat ataatacact actatttgga ccagcaatat taatttaatt tacattgaaa
     4921 tttacaaatt tctatatgta aattaaatta atattgctgg tctttatttt tctattttaa
     4981 gaatattaac ccttaactct aaatataatt taataagatt agatgtatag gaggaataat
     5041 atgaataata acatatacga agatatatcc aaaaggactc agggggatat atatattgga
     5101 gtagttggac ctgtaagaac aggaaaatca acttttataa gaaaatttat ggaaaagttg
     5161 gttataccta atatagacaa tgaatttaaa aaggatagaa caagagatga gataccccaa
     5221 agtggttcag gtaaaacgat aatgacagta gaaccaaaat ttgtaccagc agatggtgta
     5281 gaaataaaaa taaaagacac agtatcttta aaagtaagaa tggtagattg tgttggatac
     5341 atagttgaag gagctttagg gcatgaggaa ggtggaaaac aaagattagt atccactcct
     5401 tggtcacaag aagctatgac atttgaaaag gcagcagaga taggaactaa aaaagttata
     5461 aaagaccatt ctacaatagg gatagttgtc ttgacagatg gttcagttac tggaattgac
     5521 agaaaaagct atgtagaacc agaagagaga gttatacaag aattgaaaaa tcttaaaaaa
     5581 ccatttgcag tggttttaaa tacgttaagt ccaaaaagtg aggaaacgag tatgcttaga
     5641 agtgaattgg aagaaaagta tgaagttcca gttcttccaa tgaatgtggt tgaaatggaa
     5701 gaagaagata tagaggaggt tatggaagct gtattatatg attttccact tacagaaata
     5761 agaatcaact taccaaaatg ggttgaaggt cttgaaagaa atcattggat aaaaagtagc
     5821 ataataacaa cattaaaaca aagtattata gatataggta aaataagaga tatagaaggt
     5881 attatacaag gattttctga gttagagttt ttggaagata caggtgttga taatgtagag
     5941 ctaggagaag gtgttataaa tattgattta cagacaaagc aagatttatt ctataatgtc
     6001 cttgaagaaa aaagtggatt taaaattgaa ggagattatc aactgcttag tctgataact
     6061 agactatcta aagttaaaaa tgagtatgat aaaatagaaa gtgctttaat agatgccaag
     6121 ataaaaggat atggagtagt agctccttct ttagaagaac ttagtctgga agaaccagaa
     6181 ataatgaaac aaggaaaaca atatggtatc aagttaaaag ccaatgcacc atcacttcat
     6241 attataaaag cagatatatc tacagaggtc tctccaatag ttggaaatca aaatcaaggt
     6301 gaagagatga taaaatatct tatggaagta tttgaagagc aaccagcaga tttatgggaa
     6361 tcaaatatgt ttggtaagtc actacatgac ttagtaaaag agcaattaca aagtaaatta
     6421 tatactatgc cagaagagat aagagttaaa atgcaaaaga ctttacaaaa aattgtaaat
     6481 gaaggaagtt ctaatattat aactattttg ttataaaaat atagctactt attaaagtga
     6541 aacgtctgtt tactttagta agtagctttt tatttataac aattaataaa gatacttata
     6601 taaattgaca ataattgata attgtggaaa aattatagtg ggtgaagtat atgtttcgac
     6661 aggattgttt caaagctaaa aaatttctag gagtgtaaga tgaaaaagta tgagatattt
     6721 atttttatga gtataatagt tttgtgtttt ataagttttc cacataaagt ttttggacaa
     6781 gaggaaaatt gtcttgaaga ttttgaaagg tgggttcaag aaaataaaaa taataaagaa
     6841 gaaatagttt atactctttc ttgtaatatg gtaatagatg aagagtttag attttatatt
     6901 ccttatgaca gtaattttac aatagatacc aacaaatata aaatattgat aaaaaatcat
     6961 gggagattta ttattgatga taatgaattg aatataatag gtgaaggagg aaaagaagga
     7021 gtaatacata tagaaaatgg tggaagtata agtattggta taaacaacat aattgctact
     7081 gatgggacag ccttatacgt tgaagaagga ggagacttac atattaaatc atcctacgaa
     7141 tctgttgaaa aaataaaggc aaatggaaaa gatgcaatag gaatttatac tgaaaatgat
     7201 attagaatac taaataagga tattgaagtg aatggagaac atgctatagg tgtttattct
     7261 aaaggtgatg ttgagataga agaaacttca ataaaagcat atagtagcaa caatgaaagt
     7321 gggttactaa gagataataa taagttggca caatcaatta tatcagaaag taagaaagtt
     7381 tatataattg atgattataa tgagcttata ccctctatag ctaaagattc tggctataat
     7441 attgtgaaat gttgtcatag gggaattggt gtatttagtg atgaaataaa agtaagtaaa
     7501 gatgataaaa tagaaaatat aaaatttccc aaatatatgg tattagaaac atctagtggt
     7561 aatcaacttg atatagatgt agaatgggat tttactgatt attatgaaaa gttggaaaag
     7621 ggagaaaatt ttgatataac aggaaagttt aaaacagaaa tgttaaataa agaaaaaatt
     7681 ataataaatg atgatgtagt tcctattttg aatgtatctg ttatagagaa aaaaacaata
     7741 gacaatttgg aactggaatt tcaaaataca aaaaatggat atgtagctgt tttattttat
     7801 gatatgccgt atagtgcaag taaggttttt gttgaatatt caagcgatgg aatcaattgg
     7861 atatctgaag aacaggagga tattagagat caagctatac ttttttttga agattttaag
     7921 ttgcgttgtt ttagagtaaa agtggtagga ggattaaaag aagggtattc aaatatagtt
     7981 tttaaaccag gatttataat gggaggcgga gacaaccaag agacgccaga tgatgaacgg
     8041 gaaaatgatg atattgatgg tgaccgtgga ggaggaggta gagacgaccc ggatagagat
     8101 gaggatgata acaatcaaga tgggaataat ggaaatgaaa ataatgatag taataacaat
     8161 caaaacaata ataatgaaaa taataattct gatgagaaac aaccaaatac tcctaaagat
     8221 gatgtagaat caagtactga tagcaatgaa aataaaaatc ctcatgatat acctaaagac
     8281 ggtaataaag aaaataattc tgataaagaa aaaggatttt acaataaaaa tccaattaca
     8341 gaaaatgatg ataattatga tttaagctca aaatcagtat ttggtaccaa tgaacattct
     8401 tttccttata gggaaagtat ttctaacaaa aatcaagata caaataaaaa taagtcaaat
     8461 ataagtgatt atgatggaat aataaataat aattataaat tagaatctaa agaatataat
     8521 ctaaaaaaag atattatttt tttattttgt atactaacac caattatgat tggttctata
     8581 ttaattgtaa atcctgatac tagaaaaagt ataattaaat tcataaaatt aaaaaaataa
     8641 tttttagaga ggtattattt agaaaatcac gagattgcta agtaatacct ttcattatta
     8701 tgaaaactgt atttattgtt gagaaaacat atataaaata tgaattttct aaatagtata
     8761 aaaataatgg aatattgaaa aaaaattgac aaatgaatgt ataggtatta aaatatatta
     8821 aatatataca taaaaagtgg tttactaaca aataaatgaa atatatgtta atacatagtt
     8881 caatttgtaa aaatataaga tttataaaaa aataagggag gatattatta tgaaagcaat
     8941 tttaacagta attggaaagg ataaaccagg tatagtggca ggaattagtg atgaattata
     9001 caagcaaaat atcaacatag tagatatatc acaaaaaata ttacaggatt attttactat
     9061 gattatgata gttgatttag agaaatcatt aaattctttt gagaaaacgg ttgaggactt
     9121 ggttgaaaga ggtaagaaat tatcagtgga tgtaaaaatt cagcgagaag aaatatttaa
     9181 ttcaatgcat aatctgtagt agacagtaag gggaggtgat aatagtgaat gtaggaaata
     9241 tacttgaaac aataaagatg atagatgaag aacagttgga tataagaact ataactatgg
     9301 ggatttcttt attagattgt atagaccctg atggagataa agctagagaa aaaatataca
     9361 ataagattat gagttctgct aaagatttgg tgaaggttgg aaaagatata gagcgtgaat
     9421 ttggtatccc aattgtaaat aaaagggtat cagtaactcc aatttccatt attgcaggtg
     9481 caacagatga aaatgactat gtaaagtttg cacagacatt ggataaggct gcagaggatt
     9541 taggtataga ttttattggt ggattctcag cattagttca aaagggatat acaaaaggtg
     9601 ataagatact aattaagtca attccaaaag ctctagcaag tactaataaa gtatgtgctt
     9661 cagtaaatgt aggttctaca agatgtggta taaatatgga tgcagtgaaa gaaatgggag
     9721 aaatcataaa agaaactgca gaattaacaa aagatgcaaa aggttttggt tgtgcaaaat
     9781 tagtagtatt ttgtaatgca gtagaagata atccatttat ggcaggagct ttccatggtg
     9841 ttggtgaggc tgatagaatt ataaatgttg gtgtaagtgg ccctggtgtt gttaagagag
     9901 ctttagagaa agtaaaggga gagccttttg atgttgtttc agaaacagtt aaaaagactg
     9961 catttaaaat tacaagagtt ggacaacttg tggcaaagga agcttctagt agattggatg
    10021 ttccatttgg gataatagac ttatcactag ctcctacacc tgctattggt gattcagtag
    10081 caaatatatt agaggaaatg ggtcttgaag ttgttggtac tcatgggact acagcagcat
    10141 tagcactttt aaatgatgct gttaaaaaag gtggagtaat ggcatgttca catgttggag
    10201 gacttagtgg agcgtttata ccagtatctg aagatgcagg tatgattgat gctgttatta
    10261 aaggtgcttt aagtatagat aagcttgaag caatgactgc tatttgttct gtaggtcttg
    10321 atatgatagc tgtgccagga aatactacag caggtacgct tggagctatg atagctgatg
    10381 aggcagcaat aggtatgatt aataataaga ctacagccgt tagaattata ccagccccag
    10441 gatgtgatgt aggtgatatg gtagaatttg gaggactttt aggacgagca ccagtaatgc
    10501 ctataagtac aaattcatct gaattattta ctcaaagagg aggaaggatt ccagctccta
    10561 tacattcatt taaaaattag ttatattaca tataataata tttaattaaa tatttagatg
    10621 gttaaattaa agaagtctat gcatttaaat attatataag ataatgaact tcgttaagat
    10681 aataaacttt aaaaatcagt tgttaagatt atggcaaaaa taaagcgata catggacatt
    10741 taaaaattca tgtatcgctt ttattttaat aatatctaat taatataaaa
//
LOCUS       DAEPOZ010000129        10691 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.129, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000129 DAEPOZ010000000
VERSION     DAEPOZ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10691
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.129"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..235
                     /locus_tag="KR440_002437"
     CDS             <1..235
                     /locus_tag="KR440_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH0699475.1"
                     /translation="RQLEYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKDLNIRE
                     WICPSCNEIHDRDINASINILKEGLRLITIQNK"
     gene            519..965
                     /locus_tag="KR440_002438"
     CDS             519..965
                     /locus_tag="KR440_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBH0699476.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            986..2029
                     /locus_tag="KR440_002439"
     CDS             986..2029
                     /locus_tag="KR440_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBH0699477.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
     gene            2800..3960
                     /locus_tag="KR440_002440"
     CDS             2800..3960
                     /locus_tag="KR440_002440"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBH0699478.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKRSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
     gene            3962..5092
                     /gene="glgD"
                     /locus_tag="KR440_002441"
     CDS             3962..5092
                     /gene="glgD"
                     /locus_tag="KR440_002441"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HBH0699479.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGS
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            5121..6563
                     /gene="glgA"
                     /locus_tag="KR440_002442"
     CDS             5121..6563
                     /gene="glgA"
                     /locus_tag="KR440_002442"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBH0699480.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNRDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            6577..8997
                     /locus_tag="KR440_002443"
     CDS             6577..8997
                     /locus_tag="KR440_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HBH0699481.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNIDNLFDKVAIHINDTHPTLCIPELMRILL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYEQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            9002..>10691
                     /locus_tag="KR440_002444"
     CDS             9002..>10691
                     /locus_tag="KR440_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HBH0699482.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDIPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVDENNDRMLI"
BASE COUNT         4312 a         1052 c         1978 g         3349 t
ORIGIN      
        1 tcgtcaatta gaatacaagg ctaattggca tggtagacaa attgtaaagg taggtaaatt
       61 ctttgcaagt tcacaaatat gcaataaatg tgggtataaa aacgaggaag tgaaggactt
      121 aaatataaga gaatggattt gtcctagttg taatgaaatt cacgatagag atataaatgc
      181 aagtataaat atattaaaag aaggattaag actaataacg attcaaaata aataacttat
      241 gtaagaaccg taggaactac ggggatagcc tgtggagaat tagtaagaca tatgttagta
      301 tgcaaaattc tatgaagcag gaaccctgtg actttagtca tgggaggttc agactacttt
      361 gtaacaattt taacaaagta tgttgatata atactattaa attaatctta gattagttta
      421 ttctattgat ttgtagttta aatttatatt caaaaacatt gtataaacat ataatttata
      481 tagggtttta taattcatat gaaaagaggg tagatagtat gaagttaata tggttgatag
      541 tagcagtctt atttggaata gcagaaatgt taacaccaag cttaacattg atatggttta
      601 gtgtaggagc tgtaatactt atatttttaa gtagctttat agagagtatt ttcttacaaa
      661 ttttaatatt tgcagtaatt tctatagcaa tgttagtcgt agctactaaa aaaatagtaa
      721 aaaaagataa aggctataaa tctaacacta atttacaagc aatgatgtct aaaaaaggta
      781 ttgttacaga agagatttct ccaaataaca caggtctagt tgtagttgaa catgaaacat
      841 ggacggctat ttctatagat ggtgaaaaga tagaaaaagg ttctacagta gaagtattaa
      901 aaattgaagg ggtaaaatta gttgttaaaa aagtagacgc cacagttaat gtgacaaacc
      961 aatagaataa aggaggattt atagtatggg tactaaaatt gttttaagta ttgtattaat
     1021 agtagtagtc gtagcaataa gtttaacgtg tataagagtt ataaaacaat ctaaagtagg
     1081 tataataatg agacttggta agtttcaaaa agtggctgaa acaggtgtac atttcttaat
     1141 accattttta gataaaatgg catatgtaat tgaccttaga gaaattgtaa tagattttcc
     1201 acctcaacca gttataacta aagataatgt aactatgcag attgatacag ttgtatatta
     1261 taaagttaca gatccagtta gatatgtgtt tgagatagct aatccaatag ctgctattga
     1321 aaatttaaca gctacaacac taagaaatat aattggtgaa cttgatttag atgaaacatt
     1381 aacatcaaga gatataataa atgtaaaaat gagaactatc cttgatgaag caacagataa
     1441 atggggtata aaagtaaata gagtagagtt aaaaaatata atgcctcctc aggatattca
     1501 agttgcgatg gaaaaacaaa tgagagcaga aagagaaaga agagaggcaa tacttcaagc
     1561 agaaggtaat aagtcagctg caatacttca agcagaaggt gaaaaacaat ctgcgatatt
     1621 aacagcagaa gccaaaaaag aagctatggt acgtgtagca gaaggtgaaa aagaatctgc
     1681 tatattagtg gcagaaggtg aagctgaagc tataagacag accgctattg ctaaggcaca
     1741 aggtgaggct gaaatgataa aaagaactca aatggcaaca gcagaaggtt taaaattagt
     1801 tttttcagca atgaaagagg ctgatataga caataatatt ctagcattaa aatctatgga
     1861 agctcttgaa aaaatggctg aaggtaagtc aacaaaactg gttttacctt cagaagcagt
     1921 taatttccta ggaacattca aaggaataaa ggaagttatg agtgatgata acaaagaagt
     1981 acttgatata aaagaagttt taaatgataa tgaatcatta aaaaaataaa agagttttgt
     2041 tatcatagga aggatatgga aattaaaggt cgaattaatt aaatatagca caaaaatgca
     2101 cgttgaaaag aagaggactt ataaattttt aagtcttctt ctttgttttt attagaaaga
     2161 tgaaattata aaaatgaatt aacagaatct ttatacaatt tatcctttac taatgtaaaa
     2221 agtgttatat aatattatat aaaacatata gcaaataagc atggttttag ttagtctatt
     2281 tatattatta atatatattt ataaaaatat aaacattaat actaagaaaa ctaataaata
     2341 atttaaaagt ttatatcctt ttgtttaaat tatagacaaa gaagtatcaa ttaaaaaata
     2401 aagttgatta ataattactt gattttcttt ggataattat ttaacaaata ttagattata
     2461 ttttgctatt acgaatttaa caaattaaaa aataagcatg tttatatgac acaagggatt
     2521 tataaaaaat gtttttatat ttataaaata aaatttgtat tattttttaa ccgataattg
     2581 aatattaata aatactatag tgcttatgga aaataatgta tacgtcggta agttcattat
     2641 ggggtataca ttatatcaat tgtatagttg atattatttt atttttatta aggtattatt
     2701 taagcaacat ttatacacgg catttattga ctgataaagt ttataaaagt aaaaatgcaa
     2761 ttataaatat taattactag atgaggggag tgtcattaaa tgaaaaaaga gatgttagct
     2821 atgattttgg caggagggca aggttcaaga ttaggtgttt ttacaaaaag aattgccaaa
     2881 ccagctgtat catttggagg taagtataga attatagatt ttgtgttaag taattgttca
     2941 aactctggta ttgatactgt aggtgtttta actcaatata gaccgttgat attaaattca
     3001 catataggca tgggaagtca ttgggatttg gacagaataa atggtggtgt ctatgtttta
     3061 caaccattta tgaatgaaaa agaaggaaat tggtataatg gtacagcaca tgcgatatat
     3121 caaaatatgg actttgtaga tacctataat ccagaatatg tattgatatt atctggtgac
     3181 catatataca agatggatta tagcaaaatg ttaaaatttc ataaggaaaa acgctctaaa
     3241 gctacaatag cagtgattga agttccttgg gatgaagctt caagatttgg gataatgaat
     3301 acaaatgaag attcaagtat ctatgaattt gaagaaaaac caagtgaacc taagagcaat
     3361 ttggcatcta tgggagtata catatttgat tggaaaatgt tgagaaatta ttttaaggaa
     3421 gcagaaaaaa atccagaaat aaattatgat gattttggaa aaaatttaat acctaaaatg
     3481 ttagaagata atgttggaat gtatgcttat ccatttaaag gatactggag agatgtagga
     3541 actatacaaa gtctatggga tgctaatatg gatataataa aatctccaga aacacttgat
     3601 ttagcagacc ctaaatggaa gatatataca aatactatgg caatgcctcc acaatatata
     3661 ggtaaaaatg caaatgttca tagatcaatg atagctgatg gttgtagaat tttaggtgaa
     3721 gtaggcaact ctgtattatc acatggagtt gttgttggta aaggaagtaa agtaattgat
     3781 tcagtaataa tgccaaatgt tgtaataggt gaaaatgtaa ctatagaaaa agctatgata
     3841 ggtgagtgtg caactataaa tgataatgtt cagataaaga atgtaaataa tgaaataaat
     3901 gtagtttctg agtatgaaaa tatcgaacct agatgcgtat taatagaagg aggtctttaa
     3961 gatgagaaat gaatgtttag ggataataaa tttaaacaaa aaaggagacc cagcaataaa
     4021 caaacttaac tatggaagac caatagcatc tacaccaata gctggaagat atagaataat
     4081 agactttgct ttatctaata tgataaattc aggaatcact aaagttggaa tattcgcaaa
     4141 agaaaaatac agatcattga cagaccatat aggtagtggt aaagattggg atttaagtag
     4201 aaaaaaaggt ggcctatcaa tttttagtcc agaaaacact aagtatagaa atacttactc
     4261 acatagagaa ggtgatatat atactatctt agctaatcta gattatatag aaaagagtga
     4321 agaagaatat attttaatag ctccaagcta tatgctatgc aatttaaatt attctcaagc
     4381 attggaatac cataaaaaat ctcataatga tataactata atatataaaa atgtaaataa
     4441 tgcaaataaa gattttgctg gtaacttaac actaaattta gatagtaaca atagggtaat
     4501 aaatgtagga aataatttag gcaaattccc tagagcaaac atatgtatgg agacatatat
     4561 tatgaagcgt gaagattttg ttgaatgtat atataacatt gtaaataaag gcagttattg
     4621 ttatttagaa gaatttatta ttgaagaagc agaaaatatg aaaataggag cttatgaata
     4681 tacaggatat ctaaaatgtg taaattctgt tgaatcatat tttgaaatga gcaaagattt
     4741 attaaattta gaagttgcag atgaattatt atactctgaa agaaagatat ttacaaaaga
     4801 aaagaatgaa tcacctacaa tttatacaga tagtgctaaa gtagaaaata gctttatagc
     4861 atctggttgt ttaatagaag gtactgtaaa agatagtatc atatttagaa aggtaaatgt
     4921 tgaaaaagga acagttatag agaactcaat agtaatgcaa aattgtgtaa taaagtcaaa
     4981 tgcaaaacta tataatgcaa tattagataa aaatacgagt gtatcaaatg ggaaagaatt
     5041 aagaggagat gaaaaatatc ctcttgtagt ggaaaagaat acaaatatat aaataaattt
     5101 tttagaaggg ggaatgacta atgaaggttt tttatgtaac agcagagtgt tggccctttg
     5161 caaaaactgg aggattagga gatgtttctt atgcattgcc taaagaatta aaaaaagaag
     5221 gtgtagatgt aagggtaata atgcccaaat attcaactat accaagttat ttaaaagatc
     5281 aactaaaaga aatagctgtg tttagtgtac gtgttggatg gagaaatcaa tactgtggtc
     5341 ttttagaaat ggaattggat ggtgtaaaat tttactttat agataatgaa ttttatttta
     5401 gaagagaaga tgaaagaaag agtatctatg gatatggaga tgatgcagaa agatatactt
     5461 tctttacaga tgcagtttta gaagctataa gtagaattga tttttatcca gatgtaatac
     5521 atataaatga ttggcataca gggatgttac cattaattct aaaagaaaga tatgcaaccc
     5581 ttgaggggta taaaaatata aaaactatgt atactataca taatctccaa tatcaaggtg
     5641 tatttgataa gcatgtttta tatgatatat tggacttacc acaaaaatac tttgataata
     5701 gagatataga gtattatggc tcaataaatt ttatgaaagc tggtataaat tttgcagata
     5761 aaattataac tgttagtcct acttatgcaa atgaaataca aacatctttt tatggtgaac
     5821 aattagatgg attacttaga aaagagtcag gaaaattaaa aggtatatta aatggtatag
     5881 attatgattt aaacgaccct gcaaaagata aagatatatt tgttcattat gatgtagact
     5941 caattgacaa aaaagtagaa aataaattga gattacaaga tatattggga cttaaaaaag
     6001 attcatctat tccgttgata ggtatagttt cgagattagt ttctcaaaaa ggatttgatt
     6061 taatagcata catgatgcca gaacttatga gagaagattt acagattgtc gttttaggaa
     6121 ctggagaaca tcaatatcaa tcaatgttca actattacga ttctaatttt tcagataaag
     6181 tatcagctag aattactttt aatgcatcat tggctcaaca gatatatgct gcaagtgata
     6241 tgttcttaat gccatcatta tttgagcctt gtggtatagg tcaaatgtta gctatgagat
     6301 atggttcttt accaatagtt agagagactg gagggcttag agatacagta acaccttata
     6361 ataaatttac tggagaaggc aatggattta gttttaaaaa ttacaatgct catgagatgt
     6421 ttttctgttt gaaaaatgca atcaaagtat ttaaggacaa agaaaaatgg attaaattag
     6481 ttgaaaatgc aatgaaaaca gataatagtt ggaaaaaatc agctaaggag tatatagaaa
     6541 catatagaga catatgtgat taaaggagtg gtttttttgg taacaataag caaaaagaaa
     6601 ttcaagcaaa aatttgaagt aaaaatgtat agcttatatg cacaatctat caaggaagct
     6661 actgatgaac aattgttaaa tgtattatgt agtctattga aagatgagat agcaaaaaaa
     6721 tgggttgcta caaagttaga taagaaaaaa gaagtttatt attttagttt agaattttta
     6781 atagggagac agttaaaatc taatttatta aatttaaata ttgaagaaga agttagagaa
     6841 ggtttatctg aattaggaat caacttggat gatttaattg aagctgaagt agacccagcc
     6901 ttaggaaatg gtggacttgg tagattagca gcttgctttt tagattctat ggcatctttg
     6961 aacataagtg gtcaaggata tggaataagg tataaatatg gtctgtttga acaaaaattt
     7021 gtaaatggtt atcaggtaga agtgcctgat aactggctta cagaaggacg atatgcatgg
     7081 gaaactgtaa gaccaaatga agcaacaatg gtgaagtttg gtggtgaggt agaattaatc
     7141 aaggaaggta gtcatttaaa agtaattcat aaaaactacc ttcctgtaat ggcaatgcct
     7201 tatgatatac caataatcgg ataccaaaac caatgtataa atactttaag attgtttaag
     7261 agtgaaatac caaagagaga ttttggtgaa cttacctcaa atgctctaaa ttattctggt
     7321 agctatgaag aggctttaaa acataagtat tatactgagg aaatatcaca ggtattatat
     7381 cctgatgatt caaactatgc aggtaagttg ttgaggttga aacaagaata cttttttgta
     7441 agtgcaggga tacaagatat aataagaaaa tataaaaaga acaaattaaa tattgataat
     7501 ttattcgata aggtagcaat tcatataaat gatacacatc ctaccttatg cataccagaa
     7561 cttatgagaa ttttgcttga tgaagaaaat ttatcttggg atgaagcatg ggaaataact
     7621 aaaaaaacag tgtcctatac taatcatact ataatgtcag aagcaatgga aaaatggcct
     7681 gtaagtatga tgaaagaact attaccaaga atttatatga taatagaaga aataaataga
     7741 agatatgtag aggaattaaa taataaagga tatgagcagg atagaataaa aagaatgagt
     7801 ataatagact gtgacaatat caatatggca aacttatgta ttgtcacaag tcatagtgta
     7861 aatggagttg ctaagcttca tactcatatt cttgaaacag aggtattaaa agatttctat
     7921 caagatgaac caaataaatt taataataag actaatggaa ttgcacatag gcgatggctt
     7981 ataagttcaa atcctcaact tagcaattta attacagatt taattggtga ttcatggaag
     8041 aaggatacct tacaattaaa aaatattgaa aaatttaaaa atgattcctc tgttttacaa
     8101 agacttgatg atataaaata caataataaa gcaaatctag caaagtttat aaaagataaa
     8161 tacgatttaa atgtagaccc aagttcgata tttgatgttc aagttaagag attgcatgcg
     8221 tataaaagac agttactgaa tatatttaat gtacttcata tgtatcatga attacttgat
     8281 aatcctaatt tgaatcttga cccaagaact tttatatttg gtgcaaaagc agcaccagga
     8341 tattatttgg ctaaatgtat aattaaattt ataaattctg ttgcaagtac tataaataat
     8401 gatgttagag taaaggacaa gttaaaagta gtatttttag agaattatgg agtatcatta
     8461 gcagaaataa taataccagc agcaaatgtt agtgagcaaa tttcaactac aactaaggaa
     8521 gcatctggaa caagtaatat gaagtttatg atgaatggtg ctataacttt ggctacactt
     8581 gacggcgcaa atgtagagat atgtgaacag gttggtaaag aaaatatgtt tttatttgga
     8641 cttagtgcag aacaagtact aaattataat aaatatggtg gatattcttc acttgactta
     8701 tatcattcta atatggatat aaaaagggtg gtagatgatt taataaatgg atttattcct
     8761 aatcttggcg aagaaggaag aagtatttat aattcactca ctacttacaa tgatgagtat
     8821 tttgtattga gagattttga aaattatggt caagctcaag cagatataaa caggttatat
     8881 agagataaag aaaaatggaa taaaatgtct ttagttaata tagctaactc tggatttttt
     8941 tcttctgata gaacaatttc tgagtatgca aaagacattt ggtttaaaag ggtgtgatta
     9001 aatgcaaagt gtaatagaat ataactcttg ggataaaaat tttaaggctc cgtttggagc
     9061 tttaaaattt gatgaggaat tgaccatttg tgttaaagta aatgagggat acaatatcaa
     9121 gtctatttcc ctagaaataa atagagaaga agaaatgaga acaataactt taaatgaaga
     9181 attagataat gataaacttg gaaagtgctt ttgtggtaag attgaaaagt ttgatggtac
     9241 aggtgtttat ttttattact ttaaagttga tgttgaaatg gatggtcaga taaagacttt
     9301 attttatgga aaaaacagag ataatggata ttcttgtgaa tataattata gtgatattaa
     9361 taaatatcaa atcactgttt acaaagattt taaagtacca acatggtaca aagaaggaat
     9421 actttatcat atatttgtag atagattcaa taatggaaat cgtagtggta aggttgataa
     9481 tcctaaaaaa aatagtttca tatatggaaa ttgggaagat atacctatgt acataaagga
     9541 tagtcaaggg gatgttataa gatgggattt ccatggtgga aatttaagag ggataatcaa
     9601 taaacttgga tatttaaaaa aattaggtgt aagtatacta tatctaagcc ctatatttga
     9661 agcttctagt aatcataaat atgacacagg tgactataaa aaaattgacc caatgtttgg
     9721 ggatgaagat acatttaaag aacttattga taaagcaaaa gaaaaaggta tatcaatagt
     9781 attagatgga gtttttagtc atactggagc agatagtaaa tattttaata tgtatggaaa
     9841 ctataatagt ttaggggcat atcaatctaa agaatctcca tattattcat ggtatatgtt
     9901 tgaagaattt cctcaaaagt ataaaagttg gtgggatgtt aagacattgc caaatataaa
     9961 tgaattagaa catagctata tggattatat aatatatgac aatgatagcg ttataaataa
    10021 atgggtaaat atgggtatta agggctggag actagatgtt gcagacgaat taccaactaa
    10081 gtttataaga gagttaaaaa aggaattaaa aaaggcagat gatgactcaa ttcttatagg
    10141 tgaagtatgg gaagatgcat caaataagat tagctatggt cagagaagaa gttatttatt
    10201 aggtgaagaa ttagattctg ttatgggtta tccttttaga aataacatgt tttctttctt
    10261 aaaaggagaa attaattcat atgaactttg taatagatat atgcagatta aagaaaatta
    10321 tccaaaagaa tcatttaggt ctaatttaaa tttaattgga acacatgatg ttacaagagc
    10381 taaaacggaa ttaaatgatg atgtagattt agtgaagctt gctgtagcta tacagatgac
    10441 atttgaagga gtaccatata tatattatgg agatgaagca ggtctttgtg gaggtgttga
    10501 cccagataat agaagaactt atccatggaa aaatgaagac gaagatatgc ttaattttta
    10561 tagagatgtt ataaaaatta gaaataagaa taaactttta agttctggaa atacagaatt
    10621 tatatatact aataatgatt ctgtattttc ttttattaga gttgatgaaa ataatgatag
    10681 aatgttaatt t
//
LOCUS       DAEPOZ010000130        10598 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.130, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000130 DAEPOZ010000000
VERSION     DAEPOZ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10598
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.130"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..1627)
                     /locus_tag="KR440_002445"
     CDS             complement(<1..1627)
                     /locus_tag="KR440_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HBH0699483.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKRDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGIDKMYILKNITEFAYSKLNGYELDFGKKFTYNPNIHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKETTIENKLTSSQFTKENNHYVFKGTDYDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPKEYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFEL"
     gene            1908..4085
                     /locus_tag="KR440_002446"
     CDS             1908..4085
                     /locus_tag="KR440_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBH0699484.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYRQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKDLIDREYIE
                     KHVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALHSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANVMNIDDDLLKAGYEASIRHMFKKTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDNEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEASCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYRESDINSIIDDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            4099..4521
                     /locus_tag="KR440_002447"
     CDS             4099..4521
                     /locus_tag="KR440_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0699485.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGACPKNCFKKEEGFVVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            4928..5731
                     /locus_tag="KR440_002448"
     CDS             4928..5731
                     /locus_tag="KR440_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBH0699486.1"
                     /translation="MKKLKLIIMLALVLALLITGCSSGNNKNKEEQKTIRVATSGTYY
                     PFAFKDKDKLKGFEVDFWDEFAKRTNCKVEWVLTDFSGLFGLLEADKADVVSAQLTAT
                     PEREKKYAFSDAYNYSGTNIIVREDDTSIKSIDDLVGKKVGVGTGAVANEILKEKYPN
                     DEIKIVNYSSATLKGNYQDLEMGRLDAVVAQDVEALIAIKEQKLNLKMVEPPIQFGAC
                     SFAIQKNEKGEKWTKEINEVIEEMHKDGTLTKISEAWLGKDITKEPINE"
     gene            5724..6392
                     /locus_tag="KR440_002449"
     CDS             5724..6392
                     /locus_tag="KR440_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBH0699487.1"
                     /translation="MSNFNFEFCLSIFPFLIKALGITILISIVAFFLSILLGVILAFF
                     TQTKSKVLQYITRVYVSFFRGTPLLIQIFFFYFGFPMIFSFMKDCSPYLAIVICLGVN
                     SSAYMTEIIRASIESVDKGQQEACYAFGLSKYQSMVKIILPQAATSAVPPIVSCTLDI
                     IKGSSLGLTIGIQDLMGVAQVEAASSFKYFEIYMITILIYWAVSIILSNIQSKVEKRI
                     GKAY"
     gene            6628..7290
                     /locus_tag="KR440_002450"
     CDS             6628..7290
                     /locus_tag="KR440_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBH0699488.1"
                     /translation="MIFNMKYFLELFPIIMKSFNVTVILAISSLVFSLIIGTIVALSS
                     YYKVKVLNQICKVYVSIFRGTPLMPQLFFLYFGLAYMNDFVKNMDPVFATSIVLSLNM
                     GAYMSETIRASILSIDKGQIEAAYVMGLTNLQTMKRIVIPQAVRVALPSLFNNFIDLI
                     KGSSVAFVVGVNDIMGAAKSQGALSFKFFEVYGAVMVIYWCIITPLGWGQKKLEKKIS
                     NY"
     gene            7350..8147
                     /locus_tag="KR440_002451"
     CDS             7350..8147
                     /locus_tag="KR440_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH0699489.1"
                     /translation="MVKRNIIKCLVAVLSLTFVVAGCSKKEEVKDEKVITVASSNSAD
                     PYSYVDNGEHKGFEVDMWKEIGKRSGYKIKMEPTGFSSIFGMLDSGKVDVAANFFGMS
                     KERLEKYDASIPYGSDSVCIAVKDGNNEINKLEDLKGKVVAVSEGSQGQQVAASVNKD
                     NLFEEKVYGDGTNGLQDLDLGRVSAWIDAELTVVGDAKKANMKVRVLDEKLSVTNIAY
                     FFKKNDEKSKTIKEDVNKAIEEMLSDGTVKKISEKWFGMDVSADIQK"
     gene            8280..9455
                     /locus_tag="KR440_002452"
     CDS             8280..9455
                     /locus_tag="KR440_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0699490.1"
                     /translation="MIETKIKSIALKNREKIIKIRRQIHSNPELAFKEFKTSKLIKEE
                     LNKLKIEYIDVAGTGVLATIKGKNNGGKTILLRADMDALPIKEENDLEFKSINDNMHA
                     CGHDAHVSWLLGTAMILNDIKEELNGNVKLLFQPGEEKGGSDIVIKENVLEGVDALAT
                     GHCWPTIESGKIGIARNCAMAATNTFEITIIGKGGHGAEPHNCIDPIAVGNAVYSSIQ
                     QIVSRKIDPVVPVVVSVCSFNSGVSKNIIPDVCTLQGTIRAISKEQVIEVSKILENIV
                     RNVCRAYGADCKFEKSTGGDAVINDKNMIELGRESACKILGVENVEIIDFPAMTGEDF
                     AIYMKEKPGLFMYIGVGNKEKNINYRLHSNKFNIDEKCLSIASSLFSQLAVDYLCQL"
     gene            9564..10313
                     /locus_tag="KR440_002453"
     CDS             9564..10313
                     /locus_tag="KR440_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBH0699491.1"
                     /translation="MIKVENLHKSFKGTEVLKGINLEINQSEVVAILGPSGTGKSTLL
                     RCLNYLVEPTKGKIMIGDVKVDVEKATKKDIANLRKHTSMVFQNYNLFKNKTALENVM
                     EALIVVHKKSNKEAREISLKLIDKVGMLDRKDFYPSKLSGGQQQRIGIARAMAVNPNV
                     ILFDEPTSALDPELVGEVLSVIKSLAKEGITMLIVTHEINFAKEVADKIIFMDNGVIA
                     EEGTPDEIFNNPKNERTAKFLNIVRKEEVFS"
BASE COUNT         4124 a         1130 c         1806 g         3538 t
ORIGIN      
        1 ctaattcaaa aaaatctttt gtttctcttt ctgataaatc tataaagttt ccatctttaa
       61 gtttataaaa agtacgtttt tcttgaaaag ctttcaaaat atccttgtac tcttttggat
      121 ttatatcaga tatattaaaa ttaaattcta ggtaattctg gttagtctga ttgattgaag
      181 cttgaattga tgaagcgtta tatatttttt tcaatttaaa cttctcagaa taatatatat
      241 ctccaaattc ctttagagat tctaattgta aatctaaaaa tgtaaatagg tcatagtcag
      301 ttcctttaaa cacataatga ttattttcct ttgtaaattg tgaagaagta agtttatttt
      361 caatagtagt ttccttttgt atgttgggga gcacaaattt tttatcctca tcatcatata
      421 tatactttac atcacaacat atgttgccat caatcatatc aaagtagaac tcagatttaa
      481 aatcttttgt tatattattt tttatgttat catctaaatg aacttcttga gatatagatt
      541 ttaatctagg aactagcaat cctaaaacat ccttagaatc ctctttatca aattttaact
      601 ctttagtctt tgataatatt tgatatattt ttgaataatc atgagctttt ttatgtgata
      661 ataaatagat attgccatca tataaaagta catcattttt tttagtaagt ggaacaggta
      721 gtgaatcttt agaatataag actattttat cttctatatt tttaaaatca aaccttacag
      781 gtagagtatc atttaatatc tttggatgat attcctcttt agaaaaaata agggtaaaat
      841 ccttgtactt aatagcattt aaaaattgtt ttaaagagct tgcatttatc tccataaatt
      901 tttttgaatg tgatgaagaa aagttgtatg aaagatttgt cccatattct tctataatat
      961 ctactaactt ctcatcttca tcagcaaaat aatgaatgtt ggggttgtaa gtaaatttct
     1021 tcccaaaatc taattcatat ccattaagtt ttgaatatgc aaattcagta atgtttttta
     1081 aaatgtacat tttatcaatt cctattttaa attcagcaat tgctttattt gaatccacat
     1141 ttatcaatac atctaaatta accctttctc ttttagtatt gtctaacaaa gaaagtaatt
     1201 gcttgcttaa atctaagctt gttttagaat ttgtattaga aagcttatcc ttatctgcac
     1261 ctaatatatc tattagaata ggaggaacat ctcttttatt ttttatttgt tttaagcttt
     1321 ttatcacaat agcaactata tgcttacata taaaaatact tttgcccttt gattgacagt
     1381 cagcgcaact acaatcattt tcaattatct taaagtgctt tatatctata gttattgaat
     1441 taaaataaaa gctataatta tcttcagaat tgactccacc acgaaatgtc aacaggttat
     1501 tttgtaggtc atcaatatct atgtctatat aatcaactaa ttgattagta taatatttta
     1561 cacctcttga ccatcttgat ggttctgtat ttcttcttat tagtttatat atcatatcta
     1621 ttttcatatt attcacctta cctcactgta tatattataa tctaaatgca ttcaatttac
     1681 actctaaatt taagactaaa attaaggcag atatgaatag cttttatcta ttacaatata
     1741 tgtgtaaata acaatttact attagatttt tattgaatta tcttataaac tatgaatgaa
     1801 aaataattat ataaatagat tattaataaa tataaaatct tattattaaa aatattctta
     1861 gaaattgcac tatataaaat ataaagtatg atatttgaat aaaaagaatg tataataagt
     1921 ataataattt gtatttatta ttgggaaagg aaaatgttaa tatggatagt tatagacaac
     1981 ttaaagctaa aataccctgt aaggaaacag gtatagaaat aaagaggact ttatgtgata
     2041 tttgttctcc aggagtacat tgtggaattg atgcatatgt taaagatggt aaagtaataa
     2101 aaatagaagg tacaaaagaa catccaatga ataaagggtt gttatgtacc aaaggtcttt
     2161 gcaatagaga atatatatat agagaagata gaataaaaac acctctaaaa agaattggaa
     2221 aaagaggtga aggaaaattt aaaccaatat catgggatga agcttataga gaaatagcag
     2281 aaaaattaaa tagagtaaaa tctgagtatg gagcaaagca tgtagctttt tttacgggtt
     2341 atggaaagtg gtatagacct tttttgcaaa ggtttgcatc ttcatttggt tcacctaatt
     2401 atggtacaga atctagttca tgttcaacat cagcacagat ggctggaaat gttgctactg
     2461 gaagaaactt tacaatgcaa gatatggaga atgcaaaaac ttatttaggg tggggattta
     2521 atccatacta ctctaaattt ttaaatagtg tgaatctaat aaaattaaaa gaaaaaggtc
     2581 taaaatttat aattgtagac cctagaataa caccgacaac tcaaaaacta gcagatttac
     2641 atttaagaat taaacctgga actgatggag ctttagctct tggaatggcg aatctattta
     2701 tacaaaagga tttaatagat agagaataca tagaaaagca tgtacatgga tttaatgaat
     2761 ataaagaata tgtaaaatta tttgatttag atactgtaag tagaattaca ggagtttcta
     2821 aagataatat agtaaaagct gttgagatgt tgtcacaagg tccattttgt ataaatgaaa
     2881 gtgcagctcc aatagttcat catcaaaatg gatttcaaaa ctatagagca atgatgtctt
     2941 tatctgctat aacaggtaat tatgatagaa taggtggaaa cataccaaaa caagattact
     3001 ttgctggtaa gataggtgga ttccaaacac tagaaaaaga gtttgtaaaa gaagttctac
     3061 ctaaaaatat aaatgaaaga attggtggag aaagatttcc tctatggaat gaaatggtaa
     3121 ctcaatttca agctatggat ttacctaggc aaatagaaga aaagacacca tatgaaataa
     3181 gagctttgtt ttgtcatggg ataaatgcta gaatgtttcc taattcaggg agacttttta
     3241 aagcgttgca tagtgtagat ttttttgtga atatagattt atttatgaca gatacatcaa
     3301 aatatgcaga tatactttta cctgcgtgta catcatttga gagagaagaa tgtaagagtt
     3361 acccaggagg gtatattaca tataccaaaa aagttattga taaattatac gattcaaaat
     3421 cagatgtaga aatactttca gatttagcaa atgttatgaa tatagatgat gatttattaa
     3481 aagcaggtta tgaagcaagt ataagacata tgtttaaaaa gacgtgtgta gatgtagata
     3541 aacttaaaga aagtgattta ccattaaaat gtaaagattt taagccatat atagtaggct
     3601 catatacaag agaggggtat gatactagca cttctaggtt tgaattaaaa tctacaatta
     3661 ttgaaaaata taaagatttt ggactggatg cactaccaac atatagagga tttaatgata
     3721 atatagataa tgaatatact catttattga catcaggagc aagaatttca aatgcactac
     3781 attcaagact tcatgacatc aaactttcaa gaatattaaa aaatgaagct agttgtgata
     3841 tttccagctt ggatgcaaaa gaattgggtg ttatcagtgg agattatata aaaataacta
     3901 ctaagatagg aagtttggat gtaaaagtca atgttactga tagagtctta tataagaata
     3961 tacatatgta tcatggatat agagaatctg atataaatag tattattgat gataattttg
     4021 acccttatag tggattccct gcgtatagaa gttgcaagtg taagattgaa aaaatgaata
     4081 gataaatgga gagatataat gaaaagttta tttaaattta ataaagaatt atgtacagca
     4141 tgtggagcat gtttgcttgc atgtatagac caaaatgata ataatataga caatggtgaa
     4201 ctttttagac atgtatttac aatagaggaa ggtgaaaata tagaatattt ttcattagca
     4261 tgtatccatt gtaatgagcc aaaatgttta ggtgcatgtc ctaaaaattg ttttaaaaaa
     4321 gaagaaggat tcgttgtatt agacaatcaa aactgtatag ggtgtaaact ttgtgaaaaa
     4381 gcttgtgaat atggagcctt aatttatgga aatgatggta aggcaaataa gtgtaatggt
     4441 tgtattgaga gatataagtg tggtatggat tcaccttgtg taatggtatg tcctacagga
     4501 gcattaaaat ttaataaata aattaaatat agagagtgac ttatacttgg tataagatat
     4561 ttttacaaaa agctatattg agtttgttat aataaaaact caatatagct tttttatgga
     4621 tatagttgga tactataatg tgatttttag taatagtttt ttgctattgg acaaagactg
     4681 taaaacatat taaacttatg ttgttcaaag tcgaagtata atataataac tagaaaattg
     4741 atttatagtg ttataaaagc aaatttagta aggtataaaa taagaggcaa tcttttacta
     4801 cagtatcaat atttgggagt tatgttatgg cagagccatt aaacaattat aatggtacaa
     4861 gtgattcaac ctttgaaaat gtattatata aaaaattaaa ataagattaa aaaggagaga
     4921 ataaattatg aaaaaactta aattaattat tatgcttgcc ttggtattag cacttttaat
     4981 tacaggatgt tcatcaggaa ataataaaaa taaagaagaa caaaaaacta taagagttgc
     5041 aacatcagga acatattacc cttttgcttt taaagataaa gataaactta aaggatttga
     5101 agtagatttt tgggatgaat ttgctaaacg aactaattgt aaagttgaat gggtattaac
     5161 tgattttagt ggattatttg gattattaga agctgataaa gcagatgttg tatctgctca
     5221 acttactgca acacctgaaa gagagaaaaa atatgctttt tcagatgctt ataattatag
     5281 tggaacaaac attattgtta gagaagatga tactagtata aaatcaatag atgatttagt
     5341 tggcaagaaa gtaggtgtgg gtactggagc agtagcaaat gaaatactaa aagaaaagta
     5401 cccaaatgat gaaataaaaa tagtaaatta ttcaagtgca actcttaaag ggaattatca
     5461 agatctagaa atgggaagat tagatgcagt agttgcgcag gatgttgagg ctttaatagc
     5521 tataaaagaa caaaaattaa atcttaaaat ggtagaacct ccaattcaat tcggagcctg
     5581 ttcttttgcg attcaaaaaa atgaaaaagg tgaaaaatgg acaaaagaaa ttaacgaagt
     5641 aattgaagag atgcataaag atgggacttt gactaaaata tctgaagcct ggctaggaaa
     5701 agacattact aaggagccta ttaatgagta attttaattt tgaattttgt ctaagtatat
     5761 ttccattttt gattaaagca ttgggaataa caatactaat atctattgtt gcattttttt
     5821 tatcaatatt attaggtgta atacttgcct ttttcacaca gacaaaatca aaagttttgc
     5881 aatacataac aagagtgtat gtttcattct ttagaggaac accactttta attcaaatat
     5941 ttttctttta ctttggattt ccaatgatat tttcttttat gaaagattgt tcaccttatt
     6001 tagcaatagt aatttgttta ggtgtaaatt catctgcata tatgactgaa attatacgag
     6061 catctattga gagtgttgat aaaggacaac aggaagcatg ttatgctttt gggttaagta
     6121 aatatcagtc aatggttaaa ataattctac ctcaagcagc tacatctgca gtacctccta
     6181 ttgtaagctg tacactggac ataattaaag gttcttcttt aggtctaact ataggaatac
     6241 aagatttaat gggagtagct caagtagagg ctgcttcaag ttttaaatat tttgagatat
     6301 atatgataac gatattaatt tactgggcag tatctataat attgagtaat atacaaagca
     6361 aagttgaaaa aagaatagga aaggcatatt agatttcaat atttaaaaat gggatgatgt
     6421 agatatatta ttacttgtaa ataaattttt aattttagaa aaataaactt agtaatctaa
     6481 attattgaga aaatatagta tcaaaatgga ctgtataatc aagttaaaaa atagtttttc
     6541 tattgaaaaa ataaataaaa ttctattgta atttaatatt cgacaaagtt ttataaaaaa
     6601 caacattaag ctagggggac ttattttatg atttttaata tgaaatattt tttagaatta
     6661 tttccaataa taatgaaatc gttcaatgta actgtaattt tggcaataag ttcattagta
     6721 ttttcattaa ttataggtac aatagtagca ttatcatctt attacaaagt gaaagtattg
     6781 aatcaaatat gtaaagtata tgtatctatc tttagaggta cgcctcttat gccacagtta
     6841 ttctttttat attttggact agcgtatatg aacgattttg ttaaaaatat ggacccagta
     6901 tttgcaacat ctatagtact tagtttaaat atgggagctt atatgtccga aacgataagg
     6961 gcatctatac tttcaattga taaaggtcag atagaagcag catatgtaat gggattaaca
     7021 aatttacaaa caatgaaaag gatagtgata ccacaggccg taagagttgc actgccatca
     7081 ttattcaata attttataga tttaattaaa gggtcttctg tagcttttgt tgttggagta
     7141 aatgatataa tgggtgctgc caaatcacaa ggagcgttga gttttaagtt ctttgaagtg
     7201 tatggagcag ttatggtaat ttactggtgc ataataactc ctttagggtg ggggcaaaaa
     7261 aaattagaaa agaaaataag caattattaa gttattaaga aaatacataa aagtattttt
     7321 atataaaaaa tggtgtgagg agaagagata tggtaaaaag aaatattata aaatgtttag
     7381 tggcagtttt atcgttaaca tttgtagttg ctggttgttc aaaaaaagag gaagtaaagg
     7441 atgaaaaagt tataactgta gcttcatcta attcagctga cccatattct tatgttgata
     7501 atggtgagca taagggtttt gaagttgata tgtggaaaga gattggtaaa agaagtggat
     7561 ataagataaa aatggaacca acaggtttta gttctatatt tgggatgtta gattctggta
     7621 aagttgatgt ggcagccaat ttctttggaa tgtctaaaga aagattagaa aaatacgatg
     7681 cttctatacc ttatggttct gattctgtat gtatagctgt aaaagatgga aacaacgaaa
     7741 ttaataaact tgaagattta aaaggaaaag ttgtagcagt ttcagaaggt tctcaagggc
     7801 aacaggttgc agctagtgtt aataaagata atttgtttga agaaaaggtt tatggtgatg
     7861 gaactaatgg tcttcaagat ttagatttag gaagagttag cgcatggatt gatgcagaac
     7921 ttactgttgt aggagacgct aaaaaagcta atatgaaggt aagagtatta gatgaaaaat
     7981 tatcagttac taatatagca tacttcttta agaaaaatga tgaaaaatct aaaactataa
     8041 aagaagatgt aaataaagca atagaagaaa tgctttcaga tggaacagtg aaaaaaatat
     8101 ctgaaaaatg gtttggaatg gatgtaagtg ctgatataca aaaataaata ctttaaaatt
     8161 aaaatataaa actgtgataa ctatagataa gtatactaaa tttatctata gttatctgtg
     8221 tattacaatg tatttagatt agtctttata aattaaaatg agttatggag gtcttgcaaa
     8281 tgattgaaac taaaataaaa tccattgcac taaaaaatag agaaaaaatc ataaaaataa
     8341 gacgacagat acacagtaat ccagagttag catttaaaga atttaaaaca tccaaactta
     8401 taaaagaaga gttaaataaa cttaaaatag aatacataga tgttgctggt acaggagtat
     8461 tagctactat aaaaggaaaa aataatggtg ggaaaactat attgcttaga gcggatatgg
     8521 atgcccttcc aatcaaagag gaaaatgatt tagaatttaa atcaataaat gataatatgc
     8581 atgcttgtgg gcatgatgcc catgtttcat ggttgttagg aacagcaatg attttaaatg
     8641 acataaaaga agaattgaat ggaaatgtaa aattactatt tcaacctggc gaagaaaaag
     8701 gtggttctga tattgtaatt aaagaaaatg tacttgaagg tgtggatgca ttagcaactg
     8761 gtcattgttg gccaactatt gaatcaggaa aaattggaat cgctagaaat tgtgctatgg
     8821 cagcgactaa tacttttgaa ataacaataa ttggcaaggg cgggcatgga gcagagcctc
     8881 ataattgtat agacccaatt gcagttggaa atgcagtata ttcatccatt caacagatag
     8941 tttcaagaaa aattgaccca gttgtacctg tagtagtgtc tgtttgttct tttaattctg
     9001 gtgtttctaa aaatataata ccagatgtat gcacacttca agggactatt agagcgataa
     9061 gcaaggaaca agtaattgaa gtaagtaaaa ttctagaaaa tatagtaaga aatgtatgta
     9121 gagcatacgg agcagattgc aaatttgaaa agagtacagg aggagatgct gttataaatg
     9181 acaaaaacat gatagagcta ggaagagaaa gtgcttgcaa aatacttgga gttgaaaatg
     9241 ttgaaataat tgattttcct gcaatgacag gtgaggattt tgctatttat atgaaagaaa
     9301 aacctggttt atttatgtat ataggtgtag gaaataaaga aaaaaatata aactacagat
     9361 tgcatagtaa caagtttaat atagatgaaa aatgtcttag tatagcatct tcattattct
     9421 cacaacttgc agtagattat ttatgtcaac tatagagtac attcataaaa atatatttag
     9481 cctattttta aatttaatgc tagtatatta attttgatgt agaacatatt atttattata
     9541 tttatttaaa aaggtggata acaatgataa aagtagaaaa tttacataaa agttttaaag
     9601 ggacagaagt tcttaaaggt ataaatttag agataaatca aagtgaagta gtagctatac
     9661 ttggaccatc tggaacaggt aaatctacgt tacttagatg tttaaattat ttagttgagc
     9721 ctacaaaagg aaagataatg attggtgatg taaaagtaga tgtagaaaaa gcaacaaaaa
     9781 aagatatagc caatttaaga aaacatacat ctatggtatt tcaaaattat aacttattta
     9841 aaaataaaac agcattagaa aatgtaatgg aggcattgat agtagtacat aaaaagagta
     9901 ataaagaagc tagagaaata tctttaaaat taatagataa agtaggtatg ttggatagga
     9961 aagattttta tccatcaaaa ttgtctgggg ggcagcaaca aaggataggt atagcaagag
    10021 cgatggcagt aaatccaaat gtaatattat ttgatgagcc aacatctgca cttgacccag
    10081 aacttgttgg agaagtttta agcgttataa aatcgcttgc gaaagaagga ataacaatgc
    10141 ttattgtaac tcacgaaata aattttgcaa aagaagttgc tgataaaatt atatttatgg
    10201 ataatggtgt aatagctgaa gaaggaactc ctgatgaaat atttaataat cctaaaaatg
    10261 aaagaactgc aaaattttta aatatcgtta ggaaagaaga agtatttagt taaaagtgta
    10321 taaattaagt attagaagat atgtaagata ctcccattaa atttataagt tagataataa
    10381 atttaatgga agtatctttt tattattaga tttattattt aatttaaaat tagaaatgta
    10441 actatttgaa aattcttttt aaaagtatat caaaaatatc aagtacatga aaaaataata
    10501 tttgacaaaa tatcatatat gtttacaaaa ttaacttgat ggaatgataa agcataggaa
    10561 ttacaatgag tatactaaaa ctttatatta aagtacaa
//
LOCUS       DAEPOZ010000131        10298 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.131, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000131 DAEPOZ010000000
VERSION     DAEPOZ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10298
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.131"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..108)
                     /locus_tag="KR440_002454"
     CDS             complement(<1..108)
                     /locus_tag="KR440_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBH0699492.1"
                     /translation="MKSLVLYSSLTGNTKKIAYAIYDEIQEEKDIKDVNE"
     gene            complement(196..1290)
                     /locus_tag="KR440_002455"
     CDS             complement(196..1290)
                     /locus_tag="KR440_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HBH0699493.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVIKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMKMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGGTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKDEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            complement(1292..1738)
                     /locus_tag="KR440_002456"
     CDS             complement(1292..1738)
                     /locus_tag="KR440_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBH0699494.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEG"
     gene            2044..3234
                     /locus_tag="KR440_002457"
     CDS             2044..3234
                     /locus_tag="KR440_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH0699495.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
     gene            3404..4366
                     /locus_tag="KR440_002458"
     CDS             3404..4366
                     /locus_tag="KR440_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBH0699496.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHIFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVLSPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(4484..5146)
                     /gene="zmp1"
                     /locus_tag="KR440_002459"
     CDS             complement(4484..5146)
                     /gene="zmp1"
                     /locus_tag="KR440_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HBH0699497.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHAYSTTIQQNKDTLSQIVVF
                     PTGNYNKNEANAMVNRLANIDGKYLNVLKQDNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAEAFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     regulatory      complement(5246..5331)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(5517..8471)
                     /locus_tag="KR440_002460"
     CDS             complement(5517..8471)
                     /locus_tag="KR440_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBH0699498.1"
                     /translation="MKKGNKKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEDNSTDNKKPEQTSEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQTPEGEKSIDNKKPEQIPEEDKSTDNKKSEQALEDEKPL
                     DNKNTEKTPEEDNLLEDENLLKVLEEELNEDYGFVVKINNNTIETESMKKISFNLTYT
                     PTSKGIQAGDSITFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGK
                     IGLEAYVKKVDEDTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEG
                     RKSTTVFMPVKNKDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEV
                     SDFNIEQSKNSISVNFGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQED
                     DATVSIFDRGDNYLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGM
                     RLTTSGEDGHDFRVVEVPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTI
                     NLSKEEVESKFFIAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKK
                     EVDKKVLNSSDNQIVKYKINMSTYGVYDAGQVNLLDEVNPSLKISNIKYSDNLELKKE
                     AGDGKNTIRLVNKYEFKQIKEGEPVQSWVTFDADFTNVKVGETIQNVAQINGSSPPGV
                     ETTKQGYAFEAKKVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGVIQSSVKKPGI
                     YYLVEVMAPSGYEKLKDKIKVEIGNKDIGRIVDIGNIENIRQENPPVNPPVPPNTDEP
                     IVNPPVPPSTDKPRKPSNPSDTEDTIVINPPVPPSEDIINPPIPEILNPPVPPSEEVI
                     ETPVKQIVPIPEVVKPSVSEEKNNKAKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIV
                     GLVVLRRNKNTN"
     regulatory      complement(8584..8669)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(8795..9973)
                     /locus_tag="KR440_002461"
     CDS             complement(8795..9973)
                     /locus_tag="KR440_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBH0699499.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMFTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIVSIMIKIWLSRFNKFVGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKDMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
BASE COUNT         3244 a         1659 c         1203 g         4192 t
ORIGIN      
        1 ttcatttaca tctttaatgt ctttttcttc ttgaatttca tcatatatag cataagcaat
       61 ctttttagta tttccagtta aacttgaata taaaactaaa cttttcatga tgtctcctcc
      121 tctttatcta aatacttatc taaatcacca aaaatatatt aaccttaaca aattatttat
      181 ttttataaag atattctaat ctagtataca tccaaactga gagcgtgttt gtaaagcatc
      241 tgcacacttc ttattacaat aacagcactt tttaatctca tctactcttc catcttttac
      301 tttctgtaac caattactat ctgctagtaa ttgtcttgac atagaaacca tatctactct
      361 attatttgaa ataatacttt ctataaaatc tgggctagac aattttccaa ccccacatac
      421 tggaagattt gtgtgtttct ttacttcatc agctaaatat aaaaaacagc cttcatcttt
      481 aaagtaagga tgattattgg ttggtatagt atcttctaat tttgaatgat ttgcaagtgt
      541 tacatgaaaa ctgtttacac ctagggattc aaatttttta acaaaatact caacttccga
      601 aagtaaaact cctgcatttc cataatgagg attttcttgt cttattgcaa atttatagtc
      661 tattggaatg tccttaactt catctcggat actactaatt atttcgcttg caaatctact
      721 tctattatca cacgttccac catattcatc atttctatta ttaaatatac ttgatgaaaa
      781 acttcctaca agcctatcac catgaatttg aatcatgtca aaacctgctt tttttgcatt
      841 caatgctgtt actttaaata aatttataat atttttaatt tcttctttgg gcatatttgt
      901 tatgtaatct tttactccgt cattcattat tttttttatc tcatcatgtg aagtaattcc
      961 catcttcata gtatctttaa ttaaattaac atcataatct gaacaaaata gttgtgctga
     1021 tacctttgca ccagaattgt gtatttcatc tacaattttc ttgtatttaa tgactccatc
     1081 ttcatccgat aggcttatag ctaccttatg aaaactaggt ctaatagata catctcctag
     1141 agtaatcaga gctactcctg acttagcaat atcagaaaac tttttaattt tttcttccaa
     1201 tttaagaccc atgcttgtag gtgcaaaaga tactctattt tttaattcca aatttccaat
     1261 tttaattggt tttaataaat tacaatacat cctatccttc ctccacagct gtttttatat
     1321 cctttaccac atgtaaattc tttaccagtt ctttatattc tgtatctgag aactcttttt
     1381 ttatgtggtt atcccacttt gaaaatatgt tttttatatc ccctaatact tcctcaccct
     1441 tatctgttac tctcaactga aaagctctac tatcattagg gttttggatt ttttctatgt
     1501 atccatcttg ttctaaccta gatatagttc ttgttgtatg agctttatca acttcaaatt
     1561 ctgtagaaac atcattcaaa gaacaattcc tatttttatt aatataaatt aaatagaaat
     1621 atagaccatt cgtaagttta tatggtttta aacatttttc acaataaagt ttaaagtttt
     1681 tttgcaagtt acaaataata tatgacaaac tttcatactg taaattttta aaatccatct
     1741 taaatttccc ttctataatt ataatatgtt tgactatatc aactatatta tattaaacaa
     1801 ttaatatagt caagaatatt ttattctcta aatctatgtt ttattaattc tttaaaaaac
     1861 tattatataa aataatcatg ataaaattag aaattggaat ttttaatatg cactgtacat
     1921 aaatatattt tatataaaag tttatatttt caaacaagta atttttcgaa aattgtctaa
     1981 attcagtaaa aatatggtaa tatatacata agcaatataa atctataaaa aggagtgtta
     2041 catatgatat ctaataaaat gcaaacttta gttgcaaata gttctgttat aagagctatg
     2101 tttgaagaag gaaagaaact ttctgatatt tatggtgagg aaaatgtttt tgattttagt
     2161 ataggaaatc caagtgttga acctccagag actattaaag cagttataaa tgatatttta
     2221 aatgaagaat ctccaaattt agttcatgga tatatgaaca actcaggata tgaggatgta
     2281 agagatgcta ttgctgaaca tataaataaa aaagatggat taaatcttac aagagaaaat
     2341 ttaattatga cttgtggtgc tgctggtggt ctaaatatta ttttaaaaac tcttttaaat
     2401 cctggtgatg aagttattgc ttttgcaccg tattttggtg agtataaaaa ttatacagaa
     2461 aactatgatg gaaaattaat agaagttcct acaaatatag agacttttga accagattta
     2521 gatgctctaa aaaatgctat tactccaaaa acaagagctt taataataaa cactccaaat
     2581 aatcctactg gtgtcatata ttctgaagaa ttattaaaaa atcttggaga attattggat
     2641 tcaaaacaaa aagaatttaa tacaagcata tatctaattt ctgatgagcc atatagagaa
     2701 ataatttatg atggtgccaa agttccttgt gtactaaaat actatagaaa ttcatttata
     2761 gggtactctt atagtaaatc attatctcta cctggtgaac gtataggata tattgttgct
     2821 aatgggcaaa tggatgattt tgatgatgtt atgtcttcat taaatgttgc aaatagaata
     2881 cttggatttg taaatgctcc ttctcttttt caaagggtta ttgcaagaag tttagatgca
     2941 gaagttgatg taaatattta taaaaagaat tttgaccttc tatataatag tttaatcgat
     3001 atgggatatt cttgtgttaa acctaatggt acattctatt tgttcccaaa agctccaatt
     3061 gaagatgata aaaaattctg caatgatgca aaacaattta atttattatt agtaccaggt
     3121 tcttcttttg gttgtccagg tcattttaga gtatcatatt gtgtatctta tgataaagtt
     3181 aaaagttctt taccagcatt tgaaaaatta gccaaattat ataatttaaa ataactaaat
     3241 caaagtaatt caaataatta actaccttag tatagaatac ttacattcta tattaaggta
     3301 gtttataatt ttatgtacta tattttatac ataccatatt attccaatat gcttgaatat
     3361 tcttatctat agtactaata taaattacaa ggaggtgttt gacttgaaaa aaggttatgt
     3421 ttatataata ctgactacct tattttttag ttcaatggaa attagtctaa aaactgtcac
     3481 taatgacttt aatcccttac aaataacttt atcaagattt tttgttggcg gtttagtctt
     3541 actacctttt gctgtaaaaa gattaagagc tcttagtctt tctattacaa aatcagactt
     3601 aaaatacttt gcatttttag gttttatgtg tgtagtagta agtatgtcat tgtatcaact
     3661 ggctgttcta aacacaaagg catctgtagt tgctgttttg ttcagttgta atccagtctt
     3721 tgtaatgctt ttagcttact taattttgaa ggaaaaaata tataggcaca atgtaatttc
     3781 acttatatta gaagttcttg gaattatagt tataataaat ccattgcata caaaactaac
     3841 tcttagtgga atattcttaa cattatcttc tgctattatt ttttcaatgt atactgtatt
     3901 tggtaaaaga aaaaccttaa aatttggtgg aatagttgta acttgcttta gctttatatt
     3961 tggtagtttg gaaatgttga tattggtttt actcactaaa attaatttta tcgcagatat
     4021 attaaataaa aataaacttc atatatttgc aaatatccct atttttagtg gatatacctt
     4081 acacaatatg cccattatga tttatgtatt cgtatttgtt acaggtgttg gttatgcact
     4141 ttactttatg gcaatggaag ctacatctac tagtcttaca tctttagtat tcttctttaa
     4201 accagtacta tctccaatac tagcattatt aatacttaaa gaaattatcc ctataaacat
     4261 ggttatagga attttattga tagtaattgg ttcaataatt tctattatac ctacgataat
     4321 tactcaaatg cataataaac acgttgaagc cttgtataag gagtagtaat tgataatcta
     4381 tcaagttata cataagacaa aatttattag atttaaacgc aatataaaag agatgctatt
     4441 ataatagcat ctcttttata tttattttaa atttattaaa gctctattta gctaggtttt
     4501 gcaaaaagct ataagtttgt ggacaagctg attttaactt actatttgta tcttgattta
     4561 aatagtaata tgcaaatgct tctgcaaaaa attcttctgg atatacacct aagtagttta
     4621 cattacctaa agaacgacct tctttagcaa atatttgttt gaattgtgct gatttagata
     4681 tatcatttaa tactatatgg tctattgcat gtgctgtttc atgtaattct aaatttattg
     4741 catcatgccc tttacctttg ttactaaatc ctattctaag agctactgta cttccaccta
     4801 agcctggaac atcatcccaa gtttttccag taccttccca tcccttagga actacacctt
     4861 ttaaataagc atattctttt tcatctgtta actttccact taataatttt atttttaagt
     4921 tatcttgctt taatacattt aaatactttc catctatatt agctaatcta tttaccatag
     4981 cattagcttc gtttttattg taatttccag ttggaaaaac tactatttga ctaagtgtgt
     5041 ctttattttg ttgtatagta gtactatatg catgtgcact tactggaact gaacttatta
     5101 agaatattga tattatagct attaataatt ttttacttgg tctcatatta ttccctcctt
     5161 tgagttaggc cgggagtaat aaatttcaaa ataaaaaaga tacccaagag tatctttgta
     5221 tatacaaaaa gaaattttta ctcttttcgg caactggctg gcatggttta ctaaacttta
     5281 gcccctatag ctttgcgtga ctaggtttcc ctagttttgc ctcatttaat tttatatcta
     5341 tattatagct tatatctttt taattgtcta tatccctttg gaactttttt ctaaaaagtt
     5401 attatttttt tacataaatc ttcaaaaaaa aatatcttct aacaatattt tcattattaa
     5461 atattgtttt agaagatatt ttttaatata acttatattt tttataatgt tagggtctaa
     5521 tttgtatttt tatttcttct taatacgaca agtcctacaa tagctcctat aactaaaagt
     5581 atttctccaa taattgtagt actatcccct gtttttggtg gtactggtgg atttaccaaa
     5641 gtatcatctt tagctttatt atttttttct tctgacactg atggctttac cacttctggt
     5701 attggaacaa tctgtttaac aggagtttct atcacctctt ctgatggtgg tactggtgga
     5761 ttcaatatct ctggtattgg aggatttatt atatcttctg acggtggtac tggtggattt
     5821 ataactatag tatcctctgt atcagatgga tttgatggtt tccttggttt atctgtgctt
     5881 ggtggcactg gtggatttac tattggttca tctgtatttg gcggtacagg tggatttact
     5941 ggtggatttt cttgtcttat gttttctata tttccgatat ctactattct accaatatct
     6001 ttattaccaa tttcaacctt aattttatcc ttcagttttt catatccact tggagccatc
     6061 acttctacta aataatatat tcctggtttt tttacactag attgaattac accatcttca
     6121 tcactaacta aatccttaac tactatattt ccaaatgcat cttctaaatt gaacttagca
     6181 cctgaaagta catttttatc taaagcatcc acttttttag cttcaaaagc ataaccttgc
     6241 ttagtagtct ctactcccgg aggacttgaa ccattaatct gagccacatt ttgtatagtc
     6301 tccccaactt ttacatttgt gaagtctgca tcaaatgtaa cccagctttg cacaggttcc
     6361 ccttctttta tctgtttaaa ttcgtattta tttactaatc ttattgtatt ttttccatct
     6421 ccagcttctt ttttaagttc aagattatca gaatatttta tattagatat ttttagagat
     6481 gggtttactt catccaaaag atttacttgt cctgcatcat aaaccccata agttgacata
     6541 ttgattttgt atttaacaat ctggttatca ctactattta aaaccttttt atcaacttct
     6601 ttttttgcgc ttattaatcc agaatttttc ttaaagttta tctcatcatt tttatcaatt
     6661 tcactattat ttgcatcaag gacagctcta tttatataac tatcttcaat actaattacc
     6721 tttaatgtat aagctataaa aaactttgat tctacttctt ctttactgag attaattgta
     6781 aatccagtta taactttatc tacttgccca ctttcatttc ttttttcttc tactttatat
     6841 gaaatattat tggcaatttt ttctttactc caaggagtcc agctcccatt cattggaact
     6901 tctacaactc taaaatcatg cccatcctct cctgaagtag tcaatctcat accttctggt
     6961 atattatctg ttataacaac attacttatt ggtgatttat catcatttat tgaaatctga
     7021 tagtttatta cttgaccaac ttgagtaata tttgttgctc ctgaatgtcc tttagtaaga
     7081 agataattat ctcctctatc aaatatactt acagtagcat catcttcttg tatcttctta
     7141 ccatcagatt caatcctagc tacattctta tatttattac catgttttgc atttttgtca
     7201 ctgattctag ccttatactt aacagtatac gatttatcta tatttccaaa attaactgaa
     7261 atagaatttt tactctgttc aatattaaaa tcactaactt cttttccatc acttgttact
     7321 acacttacag aaccatttat caatgtcaat ccttcaggaa gctcatcata caaaataata
     7381 tcttttaact cttcctgctt ttcatttact tgtatactgt aatttatatc tttattttta
     7441 actggcataa atacagtagt tgattttctt ccttcaacaa gctttttaag tgttccttta
     7501 gcatctggaa catccgtttt atctccaggt tttatttcta catctatatc tccacttcca
     7561 ctttctattt ttccagtggt ttcaatatgt atttttgcat tagtatcttc atcaaccttt
     7621 ttaacatatg cttccaaacc tattttacct tgcattacac tttggccatt aggtaattct
     7681 ctaaatgtaa gtgtatactc tccattactt tctgttttat caaagcattc acttgtataa
     7741 tctaaattaa ctttattgaa tacatctggc accttaaaag ttattgaatc tcctgcttgt
     7801 attccttttg atgttggagt ataagttaaa ttaaatgata tttttttcat tgattccgtt
     7861 tctattgtat tgttattaat ttttacaaca aatccatagt cttcattaag ttcttcttct
     7921 aaaactttca ataaattttc atcttctaat agattatctt cctctggagt tttttctgta
     7981 tttttattgt ctagtggttt ttcatcttct aaagcttgtt ctgacttttt attatctgtt
     8041 gacttatctt cttctggtat ttgctctggc tttttattat ctattgactt ttctccttct
     8101 ggagtttgct ctggcttttt attatctatt ggcttttctc cttctggagt ttgctctggc
     8161 tttttattat ctgttgactt atcttcttct gaagtctgtt ctggtttttt attatctgtt
     8221 gaattatctt cctctggggt ctgttctggt tttttattat ctgttgcctt atctttctct
     8281 ggagtctgtt ctggtttttt attgtcgctt tccttctcct ctccagttga tgaattagtt
     8341 gaaccacttt gaatctgact attctctcct aattctgaag cataagatac agttggatat
     8401 attgtagaca ccaccatact taaaatcata attaaactaa ttaataatgc ctttttattt
     8461 cctttcttca tataaatttt ccccctctag gttatctata gtttataatc ttccattaat
     8521 atcgtatata aattaatttt tactaaaaaa ttatataaaa aaagctatga aaataaataa
     8581 atttatattc atagccggtt gcaccactga ctcagtatat ccataaggtg cccaataata
     8641 gaatatactt caacatctct ataatatttc ataaaaacaa ttaaaattgc tttatttata
     8701 ttaaaaatac aattatatac ttctattata ttatgcctct atactattgt ctatcttttt
     8761 tacatttttt accttttatg agattaatta ataattaaat aaaatcctta tctacaacaa
     8821 ttacacaatt atattcagga tttatctcct taactaattt tgttatatct tcttttaagt
     8881 catcctctga catgtctttg gaaaattcgg aaggattaac tactatatca aatattaaat
     8941 tcttcttgct tccttttcca actattctaa aatcatgcat agattttaca agactatttt
     9001 tcttaagtat ttcttcaagt tcttttttgg ctgacataac ttctttatct tcaagacata
     9061 ttgggtccat gtgaattact agatatatat ctaatttttc agatatttct ctctctgcag
     9121 tatctataat ttcatgtatt tccattatat ctatgttgga tggaatttct gcatgtatag
     9181 atgcaataca tctaccaact ccatagttat gaataattaa atcatgtgta cctattatat
     9241 atttataaga taacaatagc tctataatag aatttaccat ttcttcatcc ggtgcttctc
     9301 ctaaaagagg atttatagta tcttttacaa gagagaaacc agcataaagt atcactaatg
     9361 atactacgat accaacatat ccatctatag gaaaattagt aaatctagct acaataaatg
     9421 atataactac acaacttgaa gtaaatacat ctcctaaagc atcaagtgaa actgctttta
     9481 aggctgaaga atcaatttta tttcccacaa atttattaaa acgactcaac cagattttaa
     9541 tcattataga tacaattaaa agtataagtg gtattacttc aaattttaca ggaattggat
     9601 ttactatcct ttctatagat gactttacaa attgaagtcc aaccaacatc accataaatg
     9661 ctactattaa tgctgataaa tactctattc ttccatgacc aaaaggatgt tctttgtcag
     9721 ctggcttact tgctaattta aaacctatca tagttattgc tgaagatgcc atatcagaaa
     9781 gattattaaa tgcatcagcc attatagcta tactagatgt gaacattcct atgaatacct
     9841 ttatcacaaa taaaagtaaa ttggacaata tacctactac accagctaca taaccatact
     9901 tatttctaac atctgtattt tgaacgtttt cgctatctct gataaatgtt ttgactagga
     9961 ttttggaaaa catgaaactt acctccctgc cctttatttt attaacaaat tttataatct
    10021 atatctttat ttatgataac ataatttata tataaaattc ttaaaaatca ctctacttca
    10081 gtaaaatatt attctatata tctatttaga tacaaatttt atatttcgta aagtttttat
    10141 ggcttagtgt ctgtttaata gattctatat aagtttttac aaagattatt tattatgata
    10201 atatcatttt tcccaagtat tacttagaat atgatttttt tgttttgtct ttacatattt
    10261 taaatttata ctatatttat gtttttataa ccttatga
//
LOCUS       DAEPOZ010000132        10290 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.132, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000132 DAEPOZ010000000
VERSION     DAEPOZ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10290
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.132"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            134..2044
                     /gene="phnW"
                     /locus_tag="KR440_002462"
     CDS             134..2044
                     /gene="phnW"
                     /locus_tag="KR440_002462"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBH0699500.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNLWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIEFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            2401..3222
                     /locus_tag="KR440_002463"
     CDS             2401..3222
                     /locus_tag="KR440_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699501.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNISGTGKIEGSVKCSKFDSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGNINSVEIDTSGIINVEG
                     SIKSDIIHGEGYLKANENIDCENINLSGILECNGFLNCEEVNISLNGTSNFNEVGASS
                     INIKKGHGDYCKKFRMLEKKHINFEKVSSNLISLGLLSIRKYTESKLFANIIEADEIS
                     LENSEVKVVRGKNIKIGKGCYVESLEYSESIEIDEDSIVGEIKNISEKIKLKKDK"
     gene            3263..4030
                     /locus_tag="KR440_002464"
     CDS             3263..4030
                     /locus_tag="KR440_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HBH0699502.1"
                     /translation="MEKGSIKILGEGFISSGEYENIKIVGSSKSKGNVKSNEVKVLGD
                     AKFEGDINIKNFVVNGKTIIRGNLKAERVRVNGQLNIEGDAEIDDLVITAQQKVDGKL
                     KCNNVTIRGQLSVNENMYAQDIKIYGEMRNKSNIECEDIKVYGVIKCDGLLNGENIYV
                     YSGGGSYCKEIGATNIKIGRSKDAFSLIKIINIFSGKFKCNLIEGDTIELEDASIKDI
                     RGKSISLVDNCDVGNIEYSDNLSVSDNSTVKNSIKVG"
     gene            4038..4658
                     /locus_tag="KR440_002465"
     CDS             4038..4658
                     /locus_tag="KR440_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbD family protein"
                     /protein_id="HBH0699503.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLDDIANMFMKKDINKEFDIDFILSKEVICNYTKE
                     IFQNLYKKEQSIGKKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKI
                     YLFRKFGVPFVVGCLDYKKVKFEKDVVKIIEIDLTKEINDISIKLS"
     gene            complement(4958..5545)
                     /locus_tag="KR440_002466"
     CDS             complement(4958..5545)
                     /locus_tag="KR440_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBH0699504.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(5631..6068)
                     /locus_tag="KR440_002467"
     CDS             complement(5631..6068)
                     /locus_tag="KR440_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0699505.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            6172..7170
                     /locus_tag="KR440_002468"
     CDS             6172..7170
                     /locus_tag="KR440_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HBH0699506.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMTGKHVGRTVVDLEKI"
     gene            complement(7290..8216)
                     /locus_tag="KR440_002469"
     CDS             complement(7290..8216)
                     /locus_tag="KR440_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBH0699507.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTVLYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            8602..10206
                     /locus_tag="KR440_002470"
     CDS             8602..10206
                     /locus_tag="KR440_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0699508.1"
                     /translation="MKEKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGAANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVLDNNREFIKKYINR
                     FKVGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENNIRPVMIHAQTVRADQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
BASE COUNT         4014 a         1131 c         1778 g         3367 t
ORIGIN      
        1 acaactaaaa acagaaattt aatatatatt taacctaaat atgatgtata attaacatat
       61 gaaagttata ctttaaaagt aaaaacaatt aaaaacacaa aaattctaaa atgaatctat
      121 ggaggctata aatatgaaaa agatatatgg agaaaaaata aaagcagtag tattcgattg
      181 ggctggaaca acagtagatt atggatgttt tgcacctctt aatgtattta tagagatatt
      241 taaaagaaga ggtatagatg taacaatgga agaagcaaga aaacctatgg gaaaattaaa
      301 aatagaccat ataagagaaa tgtgtgaaat ggatagaata aaaaatcttt ggtcagataa
      361 gtttggtaaa gtgccaactg aagatgatgt aaatgaactt tatgctgaat ttgagccaat
      421 gttatttgag acattagaag agtatacgac tccaattcct catgttgttg aaactattga
      481 aaagttgaga aaaaatggat taaaaatagg ttctacaacg ggttatacaa gagagatgat
      541 gaacatagtt gaaccaaatg cagcaaagaa aggatactca cccgattttc ttgtaacacc
      601 atcagaagta tctcaaggaa gaccgtatcc ttggatgtgc tataaaaatg ctgaagcttt
      661 aggggtgtct cctatgagtt ctatggtaaa agtaggagat acaataagtg atgtaaaaga
      721 aggcgtaaat gcaggaatgt ggagtgttgc tgtaataaaa ggttcaagtg agttagggct
      781 tacacaagaa gaagtagaga acatggataa ggaagaatta aaagctaaga tgagtatagt
      841 ttcaaagaaa tttaaagagg caggtgcaca ttttgtaata gaaactatgg ctgagttaga
      901 agatatacta ataaaaatag agaatgaaac tattaaatca gattttgtac cagaaaatga
      961 ttacatatta cttacaccag gaccactatc tactactaaa tcagtaagag catctatgtt
     1021 aaaagattgg tgtacttggg atgttgaata taataaccta gtacaagatg ttagaagaag
     1081 attagtatct ctagcaaccc aaaatacaga taaatacaca agtgtattaa tgcaaggaag
     1141 tgggactttt tcagtagaag ctataatagg aagtactatt tcaaaagatg gtaaacttct
     1201 tgtaattgca aatggagcat atggaaagag aatgaaagat atatgtaatt atcttaacat
     1261 agaatttgtt gactgcacat ttaaagatat agaagcagtg gatttaaatg tagtagaaaa
     1321 tttattaaaa gaaaacaagg atataacaca tatatcaatg gttcactgtg aaactactac
     1381 aggtagatta aatcctatac aagaggtagg gaaacttgca aaagaatata acaaaattta
     1441 tatagttgat gctatgagta gttttggggg aattgagatt gatgttgaag attttaatat
     1501 agatttttta gttagtagtt ctaacaaatg tatacaagga gtacctggat ttggttttat
     1561 aatagctaat aaagagaaat tatctaaatg taaaggtata gctaaatcat tatctcttga
     1621 tgtgtatgcg caatgggaga ctatggagaa aaataatggt aaatggagat ttacttcacc
     1681 tacacatgtt gtaagagctt tttatcaagc tttattagaa ttggaagaag aaggttctgt
     1741 tgaaaaaaga tatgctagat ataaagaaaa tcagtttaca atagcttcaa gattaaaatc
     1801 tttaggattt gatactttgg tgaatgataa tgctcaatca cctgtaataa caactttctt
     1861 atatcctaaa aatgcaaagt ttgaatttat ggaattttat acttatttaa aagataatgg
     1921 atttgttatc tatccaggta aattgactga tatagataca tttagaatag gtagcatagg
     1981 tgaagtttat ccaactgata tggagagact agctgatgta atagaaaaat ttatcaatag
     2041 atagaagaag atagtgtctt ttaggttaca gaatagtgcg gagagcctac tcctttaggt
     2101 gtgaaatgat agcacaaaca gtgtataaaa agtattgaca gtagaattta aagacttata
     2161 ttttagaagt ttatacaatg aatatataaa gattgagtaa tagggatata ttttatttaa
     2221 accacaatct aagaaattag atttctagtt taaataaagc atttccctat tatttttatg
     2281 cctatttata gaaaataaat gatagttgct ttattaagta ttattatcag acttgacaac
     2341 gtaacaatgt tttgttatat taggtttaaa gataaaatac ggtaaagaaa ggatatcatt
     2401 atgaatgaag aattaagttc actaagattt gatggtgttg gaaaatgtaa aggtggaatt
     2461 tttgataatg ttaatatatc aggaacaggc aaaatagaag gaagtgtaaa atgtagtaaa
     2521 tttgattctt ctggttcagc aaaagttttt gggacaacag aatgtaatga attttttaca
     2581 tctggtttaa gtaaagtaga aggaaacata ctggcatcaa aagttgagat atcgggactt
     2641 cttagatgta caggaaatat taattctgtt gaaattgata ctagtggaat tattaatgta
     2701 gaaggaagta taaaatcgga tattattcat ggagaaggat atttaaaagc aaatgagaat
     2761 atagattgtg aaaatatcaa tttaagtgga atcttggagt gtaatggatt tttgaattgt
     2821 gaagaagtta atattagttt aaatggaact tctaatttta atgaagttgg agcaagtagt
     2881 ataaatataa aaaaggggca tggagattat tgcaaaaaat ttcgtatgct agaaaaaaag
     2941 catatcaact ttgaaaaagt gtcttcaaat ttaataagtt taggtctatt atcaattaga
     3001 aagtatacag aaagtaaatt gtttgcaaat attatagaag cagatgaaat aagcttagaa
     3061 aatagtgaag tcaaagttgt aagaggaaaa aatattaaaa taggcaaggg atgttatgta
     3121 gaaagtttgg agtacagtga aagtatagag attgatgaag attcaatagt tggtgaaata
     3181 aagaatataa gtgaaaaaat aaaattaaag aaggataagt aataaatagg tttaaaaata
     3241 aaatttaaaa tgaggtaata atatggaaaa agggtctata aaaatacttg gagaaggctt
     3301 tataagtagt ggtgagtatg aaaatataaa aatcgtggga agttctaaat caaaaggaaa
     3361 tgttaaaagt aacgaagtaa aagtccttgg agatgcaaaa tttgaaggtg acatcaatat
     3421 aaaaaatttt gttgttaacg gaaaaactat tataagaggc aatcttaaag cagagcgtgt
     3481 aagagtgaat ggtcaactta atatagaggg agatgcagaa atagatgact tagttataac
     3541 tgctcaacag aaagtggatg gcaaacttaa atgtaataat gttacaataa gaggacaatt
     3601 aagtgtaaat gaaaatatgt atgcacaaga tataaaaatc tatggcgaaa tgagaaataa
     3661 atcaaatata gaatgtgaag atataaaggt atatggagta ataaaatgtg atggtctttt
     3721 aaatggagaa aatatatatg tttattcagg aggaggttct tattgtaaag agataggggc
     3781 aactaatata aaaataggaa gaagcaaaga tgctttttca ttaattaaaa tcattaatat
     3841 tttttcagga aagtttaaat gtaatttgat agagggagat actattgaat tagaagatgc
     3901 atcaataaaa gatatacgag gcaaaagcat atctctagta gataattgtg acgttggaaa
     3961 tattgaatac agtgataatt tatcagtttc agataattct actgtaaaaa acagtataaa
     4021 ggtgggctag aaaagttatg gaagaattaa tatctaagaa agatttattg agcaaaacta
     4081 atatatctta tggtcaatta tatagatgga aaagaaaaaa cataatacca gaggaatggt
     4141 ttataaaaaa atcagctttt acaggtcaag agactttctt tccaagagat aaaatactag
     4201 aaagaataga gttaatattg tctatgaaag aagagacttc tcttgatgat attgcaaata
     4261 tgtttatgaa aaaggatatt aataaggaat ttgacattga ttttattctc agtaaagaag
     4321 ttatatgtaa ttataccaaa gaaatatttc aaaatttata taaaaaagag caaagcatag
     4381 gtaaaaaaga actgcttata ttgagcataa tagagaagtt tttagttaaa tctataataa
     4441 cttttgaaga gttaaaattg atagtgaata taatagaaga aaattttaca tcaatataca
     4501 atgaaagtgg aaagatttat ttgtttagaa agtttggagt tccattcgta gtaggatgtc
     4561 tggattataa aaaagtaaag tttgaaaaag atgtagttaa gattattgag attgacttga
     4621 caaaagaaat aaatgatata agtataaagt tatcatagtg tattatctta tataatgtat
     4681 tataataatc tacttaaaat acactttatt taaatattta gtttaaagat aagattttat
     4741 gtgagtatgt aaaagttaaa gatagattta agtaggttat agggattagt aaataataaa
     4801 atgaaccatg atgaattact ataaattgta tttaaactac attttctact tatagtaata
     4861 tcaatggttc attttattgt tattaagata atacagtatt tataaaaata cttgatataa
     4921 atatagtaaa ttgtttaaat agaattattg ccttagttta ttcaaataat gagtttttaa
     4981 aatctaagaa atatccttgt ggatgaagac atacaggaca agcttttgga gcttctttac
     5041 ctatatgtat atggccacaa tgtgtacata tccattgtat atcttcattt cttctgaata
     5101 aagtaccttc ttctaaatca gtagcatatc taccaaatct atcaccatga actttttcta
     5161 tttcagctat attttcaaat aacttagcta tagattcaaa tccttcttca cgtgctgttt
     5221 ttccaaagtt tttatataca tcatcccact cttgaagctc attatgttgt gcagctttaa
     5281 ggtgttgtgc tgtcttgtca tataaatcta cagggtatgc tcctgatata cttatatttt
     5341 caccagcaaa ttcttttaat ttagaaaaga attgttttgc atgtgatttt tcttggtcag
     5401 cagtgtaatt aaataaagac tctattatat ataatccttc tttttttgct acagatgcag
     5461 aaatattgta tctatttcta gcttgactct cacctgcaaa agctcttaat aaattttctt
     5521 tagttttact atcttttaaa ttcatattct acctctctat cataataaat ttttacaata
     5581 tttgattctt tttatttatt attccaatag gagatataat tatgctcaat ttagtcagca
     5641 tttttaaaat tatcattaat tttttttaaa attctaagaa attcttgttt ttcattttct
     5701 gtaatattat ggtatgctgt ctctttaact tgtgttgaga tgctattaaa agtatcttct
     5761 ataagcagtg ccttttttgt aagctttata tacgtgatac gtttgtcatt tgtacatttt
     5821 tgtctttcta aatatcctag gtttataagt ttattaacca acactgtaac agtggattta
     5881 tcttttccta ttttagctgc aatttctttc attgtaagta ttccattatt ttcataaagt
     5941 gcagttaaaa tatttccatg agtaggtatt aactcaccta aaccattttt actgagttta
     6001 ttttcaatga atttaatcat actagccttt gttttactta tgaaatgtac aatatattta
     6061 tcttccataa attattctcc aatctttaat ctatttaaat tagtttgaca taaaactatt
     6121 tataaactat aattagttta acataaaact aaatgtattg ggaggaaatt tatgaataaa
     6181 aaatttaaat cactatatat tacagaagaa gaaggaaatt ttagaagaaa tatagttgaa
     6241 agagaagtag atacattgcc agaaggcgaa gttctaataa aagtaaaata ttcttctcta
     6301 aattacaagg atgcactttc tgcaaatggt aataagggtg ttacgagaaa gtatcctcat
     6361 acaccaggaa ttgatgcatc tggtatagta gaatctagta gttgtgatga ttttaaaatt
     6421 ggagaagaag ttttagtaac aggttatgac ttgggaatga atacttctgg tggtttttca
     6481 gaatacataa gagttccttc tagttgggta gttaaacttc ctaaaaatct ttcactaaaa
     6541 gaagcaatgg attatggtac agcagggttt acagcagctt tatctgtgta taaatttata
     6601 gattctgtag acaagaatat gggagatgta ttagtcactg gtggtactgg aggagttgct
     6661 gtaattgcaa ctaaaatatt gattaaatta ggttattcag ttgtagtttc aacaggaaaa
     6721 ttagaagaac aaaaagaagt attactaaac ttaggtgtta aagatgttat acataggtca
     6781 gaagtggatg ataactcagg aagacctctg cttagaccaa gatgggctgg agtaatagat
     6841 acagtaggag gaaatactct agcaacagca ataaagacaa ctaattattg tggagcagta
     6901 acaacttgtg gtaatgcagg aggagttgat tttacaagtt cagtatatcc atttatctta
     6961 aagggaatta ctttatatgg tatagattct gtggattgcc caatgaactt aagattaaaa
     7021 atatgggatt tattatcaaa tgaatggaaa ttagatgatt tagatgatat ggtaaatgta
     7081 gtatcattgg aagagttaaa tgatagtata gattcgataa tgacaggaaa acatgttgga
     7141 agaacagtag ttgatttaga aaaaatatag gcgaataaat ttataattag gattataggt
     7201 tttaagcaaa aatgagatac ttgaaattta cttatgagta tctcattttt ttatgtaaaa
     7261 aaatttatgt agatgtttgt gtaaaaagat tattcatgaa caattaattc aaatacatct
     7321 ggtcttgaat agtgaccttc tgaatcaaaa tctagtctac taagtactat tttttctagg
     7381 tctaaatcag ctattagcat ttcttcttta ttgaaaatag gacctgctac atattttcca
     7441 aaagggtcaa ctatacaact tcctcctgga cacataattt ctggttctgc atctagctcc
     7501 ttatagtagt taaggtcagt tggatacata tttttttcaa catattgatt gcatccaatt
     7561 acaaaacatc ttccttctaa agcaatatgc ttcatagttg cttgccactc ttctcttgaa
     7621 tctgcagtag gggctatgta aagttttaca cccttagcat aaagaactgt tctagctaat
     7681 ggcatataat tttcccaaca aatcaacgaa cccatttttc catatggagt atcaactact
     7741 gtaagagtac ttccatctcc ttctccccaa atacatcttt ctgttcctgt aggtttaagt
     7801 tttctgtgtt taccaattag cttaccttct ggagagaaaa ataagtttgt gcaataaagg
     7861 gtacaattta tatcattacc atctctttct gttataccca ttgatagata tactcctgct
     7921 tcttgtgctg cttttcctaa taaatctgtt gtagcactag gaactggtac agaattatca
     7981 tagtatcttt tccaatcttc tctaccttcc attgttctac ttccaacaac aaatccaaat
     8041 gaaagacctc ttggatatgc aggtatgaat gcttcaggga aaactacgat atttgcccct
     8101 tttctaccag cttctttaat taatcctaat gctttttcaa cagttttttc taaatccatt
     8161 atgactggtg acgcctggac aacagcaatt cttacatttt tattttcctt gctcataaaa
     8221 aacctcctaa aaaatattta aattttttgc attgaataga ttacatgaga tgttatttga
     8281 gaactaagat acttatattg tgaataataa tgtaactgta tatttaaaat aatacatcat
     8341 gatttaaaaa attacaattg tacagatata attgagttta gatatttaaa tttaacaact
     8401 tataatattt tttcaattag tataaagcct attaatttgt cttatttaca atgaatagta
     8461 tttacttctt ggcaaacaat aattaaaaat aaaactttta tatgattaaa ctctaaagaa
     8521 aaagtttata aagaagataa tataattaag cagaaaataa tgaaaaaaat ttattaaagt
     8581 agacaacgta aggagaattt tatgaaagaa aaaatttatt acaatggaaa tatcatcaca
     8641 atggaagatt caatatgtgg agatgctatt cttataaaag ataaaataat aaaaaagata
     8701 ggaacaaaag aagaagtatt tgctttgaaa aataaagata cagaaattat agatttacaa
     8761 ggtaagacat tgatgccatc ttttatagat tcacatagtc acttgatagc ttttgctact
     8821 actctaaagc tagttccttt agaagatgca accagtttta aagacatagt aaaaaaaata
     8881 caggatttca aagagtcgaa taatataaaa aaaggagact ggataattgg attttcatat
     8941 gataataatt ttttagaaga aaataagcat ccagataaga gtgtactcga tagtgcatct
     9001 tcagaaaatc caatactaat ttcacatgct tctggtcata tgggagctgc aaatacattg
     9061 gggttaaaac agcttggtgt taccaatgaa acacgtgacc cagaaggtgg acatataggt
     9121 agagtagaag gcagtgaaga accaaatgga tatttagaag aaaatgcatt ttttaatgta
     9181 gcatctaaaa taaaacaacc atcatcaaat gagattttta attcaattga aaaagcacag
     9241 aacatatatc taagctatgg tattacaaca gctcaagaag gtttaatgga agaaaatcaa
     9301 tttaacattt taaaagcaat ggcaaatcaa aataaattaa aaatggatgt tgtaggatat
     9361 gtaaatttga aaaaaagtaa gtctgtatta gataataata gggagtttat aaaaaaatat
     9421 ataaatagat ttaaagtagg aggttataag attttcttag atggttctcc acaaggtaag
     9481 acagcatggt tgagtagacc atatgaaaat tcagatgatg gttattgtgg atatcctata
     9541 tacaaagatg aagaagtaga aaagtttata gatatttcac taaaagaaaa gatgcaactc
     9601 ttaactcatt gtaatggaga tgcagcagca gaccagttga tagatgcgtt tgagaaagtt
     9661 ttaaacttaa aagagcaaag tagtgaaaat aatataagac cagttatgat acatgctcaa
     9721 acagtaagag cagaccagat tgatgatatg aaagtaataa atatgatacc ttcatacttt
     9781 gtagctcaca catattattg gggtgatatt catatcaaaa accttggaga agatagagcg
     9841 tttaagatta gcccacttaa aacaactata gaaaagggat taatttacac tcttcatcag
     9901 gatacaccag taatagcacc taatatgctg gaaacagtat ggtgtgctgt aaatagaatt
     9961 acaaaaaaag gtatacaaat aggagagaat gaaaagataa gcccattaga tgctttaaag
    10021 ggtgttacta taaatgcagc atatcaatat tttgaagaag ataaaaaagg ttcaattaaa
    10081 gaaggtaaat tagctaattt gataatatta gatgaaaacc cacttacaat cgaccctatg
    10141 aaaataaaag atattaaagt attgcagact ataagagaag gagaagtatt gtattctttg
    10201 aagtagatga aagttagata ttttataatc tataaaaaat agttttatat aataagttag
    10261 gtagaattaa ataagattac agttaaatca
//
LOCUS       DAEPOZ010000133        10281 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.133, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000133 DAEPOZ010000000
VERSION     DAEPOZ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10281
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.133"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            204..2423
                     /locus_tag="KR440_002471"
     CDS             204..2423
                     /locus_tag="KR440_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBH0699509.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEIALEAWE
                     RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     EECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLISENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            2532..3362
                     /locus_tag="KR440_002472"
     CDS             2532..3362
                     /locus_tag="KR440_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBH0699510.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            3352..6819
                     /gene="addB"
                     /locus_tag="KR440_002473"
     CDS             3352..6819
                     /gene="addB"
                     /locus_tag="KR440_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HBH0699511.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNPTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIEN
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINVIKNYDSDDYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGDNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSTICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            6819..>10281
                     /gene="addA"
                     /locus_tag="KR440_002474"
     CDS             6819..>10281
                     /gene="addA"
                     /locus_tag="KR440_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBH0699512.1"
                     /translation="MSSPKWTKEQLEVIGSRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIANISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDIDEKGNIVP
                     SDIAVGYRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNETESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVDSPDSDY
                     YSEIEKKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFA"
BASE COUNT         4408 a          948 c         1844 g         3081 t
ORIGIN      
        1 atcttttgaa gaaaaaaaga tttatacaaa tgaaatggaa gaatattata aaaaatatta
       61 tctatacaat acagtatata atttgttatt aaatgcaaag aaaatctatg catttaaaag
      121 tgaatatgcg attaatggtt atatccaaga gagtatatta tatagtatac ttttaaaaat
      181 ctcacataaa ggagataaaa attatgatta atataaatta tagagaagac cagataccaa
      241 taataaatta cgataatggt actatggcag taccagcagt accaggtgct ggaaaaacat
      301 ttataataac aaatttagta gctaaactat tacttgaaca gaaacataag ggtggtaaaa
      361 tattaattct tacatatatg aatagtgctg taaataattt taaaggtaga ataagaaaga
      421 ttcttgaaga gaataaaata gatgatacta attcgtatga agtaatgact atacacagtt
      481 tggctgtaaa aataataaag gaaaagccag aagttgtaat gttaagtgag gattttaaca
      541 tagctgacga tttgcaaaaa agtattatat taaatgaatg tataagtaga tttagatttg
      601 aaggtggaga aagagctttt agatggtttt taaaggagca aaaagatgaa cgctggaaag
      661 aaatagctct tgaagcctgg gaaagaggtt tttttgaatt tgcaggaaat gcaataagtg
      721 aacttaagta taagggaatt tctccagata aacttgaaga aatattgtca agaggacaca
      781 aaggaatgct taaaattatt ttaccaatat ataaattgta tgatagaaaa ttaaagcaaa
      841 atggattgct agattatgat gatatattaa ttttagcaga aaaaacttta agcttagacg
      901 aagggcttag aaagaagttt caaactagat acaaatatat atttgaagat gagtgtcagg
      961 actcaaatga aattcaaggt aatataataa aaattatatc tagtgaaaat aaaaacctag
     1021 ttagagttgg agatataaat cagagtataa caggaacatt ttcatcatca gaccctaaat
     1081 tttttaaaga atttataaat agtgcagatt tctgttatag gatggatatg tcaaatcgaa
     1141 gttcaaaaga tatacttgat ttagcaaaca ctcttgtcaa gtatgttaca aaggaattta
     1201 gacaagaaga atgtagacat gctcttgaag atatgcaaat aaaaactgtg ccaaatggaa
     1261 tgggatataa agaaaatcct aatccagaac attataatat aaatgtaaag tggtatgaga
     1321 gttggaaaat tgaaatagag caaactgcta gatacgttaa gggtataaaa aataaatatc
     1381 cagataaaag tataggtata ttggttccat ttaatgaaca agtgacacaa gttgctaaag
     1441 aacttagaga gaataatcta acctttgaag aattaggacc aaattctttg aataaaagga
     1501 aggttataaa caacatagca tatattattg attttatgtt acattgtgat gatgtagaaa
     1561 agttaatagt tgtacttaat aaagttttta tagatactga taatgaagaa gaaataaagt
     1621 attttttaga agaattaaaa aactattcta ccgaagaaat tatttataat tttgaattta
     1681 gtaagttaga taatgattta atagaattta aagaagaaag tgacatatat ttaagtttta
     1741 aaagaggaat agatattata aaacagatat tagaatatcc aataataagg ttagacctct
     1801 tagttttgtt tattgggaaa aaacttaatt tagaaaaaga agataaagct gtactggatt
     1861 atatatcttt ttatataaag tatctaatat cagaaaatat aaatatggat ttagaagatg
     1921 tatataattt attgtttgat gtaaagaata gggtgtttaa tcatataata gatgtggtat
     1981 atgagataaa tggatatgaa ccagagcccg gtagtataac actatgtaac tatcataagt
     2041 caaaggggat ggaatgggat tgtgtgtttt tactaggtct tgttgaatat aatttccctg
     2101 acaatattaa tcaaaagttt cagagtgata aatggtatct aaaagaaaaa tataaaaacc
     2161 ctatggcaat tattaaatca gaagttgaag ctatattaaa aggaagtatt tcaacagatt
     2221 atgctcataa aacgaagatt gattctataa atgaaaagat tagactttta tatgtaggga
     2281 ttactagagc aaaggaaatg ttagtacttt caggaagtgc ttatagagat gaaagtgata
     2341 tagggaataa gagaaaagag caaaaaccat gtatatattt aagcagactt aatcaacata
     2401 ttattgaaaa aaggtcaaat taatatataa taagttatta aaagattgct tgaaatatat
     2461 ctaaaagggg ataaaacaat atgtacagta agtaactact aaaaaataaa gttaagagag
     2521 aagtgatatg agtgaacgat aaattaaaat atttttctta tagtcaaaat tcaatcaaca
     2581 catataaatc atgtccattg aaatttaaat ataaatatat tgatagaata aattggaaaa
     2641 atgatgatgt tggtagtaga gagtattatg aaactttaaa aacaggaaga gattttcatt
     2701 tgatttgtga aagatatttt agcaatatac ctcttggaat ctattttaat gaggatgata
     2761 aaaatagtaa aaagttttta aagtggatgg aaaatataaa aaaagttgtt cctattgaaa
     2821 aaggaaaaac gtatttgcca gaatatgaag taaggatgac cttaaatggg gatataatac
     2881 aggcaaagta tgatttagtt gtaattggaa ataacaatat agaaatatgg gattggaaaa
     2941 ccgagtctaa aaagattgac tataaacacg tagaaaatag aatacaaact attgtttata
     3001 tgtttttagc aaaagaagta atacctaaag ttttaaaaat ggacataaat gttaaagata
     3061 taaatatgag atattatcaa cctgaatttg atgacttacc aattaccatt ttatatgatg
     3121 aagaaaaaca tgaagcaaat agaaataaaa ttcaaaatta tataagtatg ataaaaaaca
     3181 ctaattatga agaacataca tacgaagaca atctatacaa tgatatagaa aaggtatata
     3241 ataaagaaga acgtatgtgc tatagaaata aaaagcattg caagtattgt gaatttaata
     3301 agttatgtaa tggcaaagaa atagattata gtattttgga ggcggaaatt tatgggactt
     3361 aggtttgtat taggtagaag tggaagtgga aaatccacct atatactaga tgagattaaa
     3421 aaagaagctc aaaaaaatga aacgacatca attatacttt tagttccaga acagtacaca
     3481 tttgaggctg aaaatagagt aagtaagcta ttcttaggaa aagaaaaaga taagtatcta
     3541 agagttaggg tacttagctt taagacacta agtaatatag ttttttctca agtaggagga
     3601 ttgacagatg taaatataaa ttctagtgga aaagcaatga tggtatatag agctattgaa
     3661 gatgtaagtg aagaattaaa tgtgttttca aaatcaaaat cgcagtctgg ttttgtaagc
     3721 tcaattacag atatgatatc agaaatgaaa caatacaata taagccctga aatgttagaa
     3781 aatatatctg gagaattgga taatgaaact ttaagtttaa aattaaaaga tataagtaaa
     3841 atatataatt cttttgaagg gaaattgcat gaaaattatg tagatgctca agatatgtta
     3901 acttcattgg caagtaaaat tgaattaagc agttatttag atggagcttg tgtttatata
     3961 gatgaattta caggttttac accaaatcag tacaatgtca taaagagtat attaaataag
     4021 tctaaaagtg ttaatatctc tttgactgta gatgatatca actatattgg gtacagtaaa
     4081 tctgatatgt tttctagaac gaaatttact tattcaaaat tgactcaatt gtgcaacgaa
     4141 gaaggcataa aaatattgcc acaagtaaat ttaaacacag gtgttataaa aagatttgaa
     4201 aaagttaagg aattacagca cctagaaaga ttttataatg cttatccata taaaatatat
     4261 tcaaatccaa ctgaaaatat aaagataaaa gagtttaaca atttatattc agaggtggaa
     4321 gagatagcca gagaaattgt acatttagtt agagataaga atgtaagata tagagatata
     4381 accattgcta caagagattt aaatagatat gattttttag tacactctat atttaatgaa
     4441 tataatatac caaactttat agataagaag agagaggcaa aaagtaatcc aatagttatt
     4501 ttaattattt cagctttaga aatgaagaat agacgatatg ggtatgaaac tatgtttagg
     4561 tatttaaaaa gtggacttat aggaattgat aatgatgata taaatttact agaaaattat
     4621 gtgttggcta atgggattaa aggtaaaaaa tggtttgatg aaaaatggga ttatagaata
     4681 acacaaagtc tttcaggtca agaaagtgaa tttgaactgg aattaaaaga aaaaataaat
     4741 gaaataaaaa atagagtatt agagcctata gtaattttac aagaaaaatt aagaggaaaa
     4801 aatagagtaa aagaaatctg tagatatata tatgaatttt tattagatat aaatatgcca
     4861 gaaactatag aaaatttaat agtaaatttt aaggataaag gggaacttga tgttgcaaat
     4921 caatactcac aagtttggga catagtagtg gacatattag accaaatggt ggaacttatg
     4981 ggtgatgaaa taatttcttt agaaaaattt ataaaattaa taaccttagg ctttgatgaa
     5041 tatgaattag gactggttcc tcctagtata gaccaggtac ttgtaagtag tgtggataga
     5101 atgaaaaatc cagacacgaa atatttatac ctagttggaa ctactgatgg tgtgtttcca
     5161 ctgattacaa aggatagtgg aatattgagt gataatgata gggaaagtct tggaaacaaa
     5221 ggaatcgaag ttgatattga tagtaagact aggagttttg aagaacagtt tcttgtatat
     5281 aaagctctta cttcgacatc taagaattta accataacat atccaatatc agaccatgaa
     5341 ggtaaaacgc ttagaccatc aataattata tcaagactta agaagatatt tccaaatata
     5401 gaaaataaaa gttatttagt ggaagaaaat aaaagcactg ataaagatat acttaaaaaa
     5461 ataacggtta aatcccctac atttaatgaa cttataaacg taataaaaaa ttatgacagt
     5521 gatgattaca atacagaaga gattaatagt atttggttag atatatatag gtactattta
     5581 aaagatgaaa tctatagttc tataacaaaa aaagttataa aaggtcttag ttatactaat
     5641 caagttcata agattgaaga gaaaaaaata aggtcattgt ataaaagtaa ttctcttagc
     5701 atatctaggc ttgaaaaata tgctgaatgt ccatttgctt attttataca atatggtcta
     5761 aaagcaaaaa agcgtaaaga gtattcattt acaccaccag atttaggaac ttttattcat
     5821 aatattttag ataggttttc aaaagaactt ttacaagata atttaacttg gagagatatt
     5881 gatgaaaaat atatagaatt aaagataggt ataatagttg atgaaataat tttaaagata
     5941 ccaggatata tattaaatag ttctgagagg tataaatacc ttgcatatag attaaagaat
     6001 atgttgacaa ctgctataac tataattagt cagcaaataa aacaaggttc atttgaacca
     6061 atagattatg aagttaaatt tggcgacaat ggaaaatatc cacctataaa aatggtgtta
     6121 gaaaatggac aagaagtaag tttaattgga caaatagata gagttgatga atttgaagaa
     6181 ggagaaaata aatatataag aataatagat tataagtcag gcaataagtc cataagtcta
     6241 acagaaatat attatggtct acaattacaa ttacttgtgt atttagatgc aatccttgaa
     6301 agtgcaaagg atgaagatat aaatataaat ccagctgcaa tactatattg tagaataaat
     6361 aatccaatag caaaatttaa tgaagataaa gatgatgaag aaatacaaga agcaatatta
     6421 aaagaactta gaatgaaggg gcttgtagta aaagactctc acatagttaa agagatggat
     6481 aaatcattaa tagatggaga aaggaaaaac tctttagtaa taccagtagg tcttactaag
     6541 gatggaaatg taggtaaaag tacttcagca ataagctatg aggatttcaa actgcttaga
     6601 aaatatgtaa ggcatgctat aaaggattta tgtgaagaaa tgttaagtgg agaaatacgt
     6661 atagctccat ataaacataa agatggaaca tcatgtgact tttgtgatta ctctactata
     6721 tgtcagtttg attcaactat aaaagataac aagtataaaa atttaaataa taagagtaat
     6781 gaagagataa taaaaatgat gaaaggagat gttaattagt gagttctcct aaatggacaa
     6841 aagagcaatt agaagttata ggaagtagag aatgcaattt attggttgct gctgctgctg
     6901 gttcaggaaa aacagccgtt ctagttgagc gtataattca aatgataaca agtagagaaa
     6961 atccaattga tatagataag ttattagttg ttacatttac taatgctgct gcgtcagaaa
     7021 tgagagagag gataggggat gctataggaa aagccttaga tgaaaaccca gaaaataaac
     7081 atttacaaaa ccaacttgtt ttattaaata aatctagtat tacaacaata cactcattct
     7141 gtttggatgt tataaaatca aactttcata gaataaactt agaccctaat ttcagaatag
     7201 gagaccaaac tgaatgtgct attttgaaac aagaagcaat agaggaagta tttgaagatt
     7261 tatatgaaga aagagatgag gggtttttaa atttagttga aagttatgca gaaagaggag
     7321 gagataaaga agtacaagat attatcttgg gaatatattc atttgcaatg gcttctcctg
     7381 aacctaaaaa atggttgatt gactcagcag aacgatttaa tattgatgaa aattttgatt
     7441 tttcacagtc tatttgggct agagctatat tagacactgt aaaaatagaa attaatggtc
     7501 tttgtctaaa tatggaaagg gcattaaaag aagtggaatc aatagaagaa ttagaaactt
     7561 ttgcagaaaa gttatccgtt gaatataaaa aaattgctaa catttcacaa gcatgtaata
     7621 aatcttggga tgaggcatat aaaaagatgg caagtatgtc ctttgaaaat tatgtaaaag
     7681 gagtaaaaag aatttcaaaa gatgctcctt cgtatataaa agaatcaaaa gaaaaggcaa
     7741 aaactataag agataagact aagaagtctt tagaaagtat agtaagtgct acattcaata
     7801 aagataatga ttctattaga gaagaaataa aatatcttta taacatagtt aaaccaatat
     7861 ctagtgttgt actgagattt gaagaggaat atagcaataa gaaaagagaa aaaggaataa
     7921 tagattttaa cgatatagag cattttgcat taaatatact tacagatata gatgaaaaag
     7981 gtaatattgt accttctgat atagccgttg gatatagaaa caagttttat gaaatattta
     8041 tagatgaata tcaggatagt aatctagtgc aggaagtact gcttaaagct gttgcaaata
     8101 cagaaactcc aaatagattt atggttggtg atgtaaaaca gagtatatat agatttagac
     8161 aagcaaagcc agaattattt ttgcaaaaat ataataacta taatgataaa aagggaagtt
     8221 cccatagaaa aataatgctt tacaaaaatt ttagaagtag agaagaagtt gtagatgctg
     8281 taaattatat atttgaaaat ataatgaatg agaatatagg agaaattgaa tatacagaaa
     8341 aagaaagact aaatttaggt gcgaatttta atgtggatac agacgagaaa tctattattg
     8401 gtggagcaac ggagatacac ctaatacaaa aagataataa acttgatgat gatattataa
     8461 atgataaaga cgatagaatt aataacaaag aaaatgaaat agaggaagaa gaaaaattag
     8521 ataatataca acttgaggct agaatggttg gaaacattat caaagattta atgaaagtca
     8581 atgaagatgg aaaaattcaa aaggtctatg ataagggaat agatggatat agacctgtgg
     8641 aatttagaga tatagttatt cttttaagag ctacatctgc atgggctcca gtgtttgcag
     8701 atgaattgat gaatatggat ataccaacat atgctgatgt tggagtgggg tattttgata
     8761 cgattgaaat aaaaacaata ttgtcattac ttcaaataat agataatcca atgcaagata
     8821 tacctcttat atctgtatta aaatcaccta tatttggatt tacaccagag gatttgattg
     8881 atattagagt tcagagtaaa gataaaattt tttatgaggt tttaaaaagt acagcagagt
     8941 atgatggatt tacagattct caaaatgaaa ctgagtctga gtttatacca agtgaagagt
     9001 gcataaacaa aagtaaagat tttttaatta agctaaagga atttaaagaa aagtcaatgt
     9061 acatgagtac agatgaattt atatggtact tgtacacaag gactggatac tatgcttatg
     9121 ttggagcatt gccaggtgga agtcaaagac aagctaattt aaaagtattg tttgagagag
     9181 caaaacaatt tgaagaaacc agtcttaaag gaatattcaa ctttgtaaat tttatagaga
     9241 aattaaaaaa atctagttct gatatgggaa gtgcaaaaac acttggtgag aatgcaaatg
     9301 ttgttagaat aatgagtatt cataaaagta aaggtctaga atttccagta gtcatatgct
     9361 ctgcaatggg taaaaatttc aatactcaag attttaaaaa gagtatttta tatcatcata
     9421 atttagggta tggacctcaa tttgtagact atgagagaag gatttctttt ccaagtatag
     9481 ccaaagaagc attaaaaagc aaaataaata tagaaaattt atcagaagag atgagagttt
     9541 tatatgttgc atttacaaga gctaaagaaa aattgattat aactggttct actagaaata
     9601 tacaagatag tataaagaga tggtcaaatg gaattgaaag tttagataca atatcacaat
     9661 acgagatatt aaaaggtaaa aattttttag attggataat gccttgtgta ttaagacata
     9721 gggatttatc taatctacta gaagaagtag gattagacgc agtttttaat gttgaacata
     9781 attcaaaatg gtatggaaag ttgtggaata agaatgatat tttagtggaa aagaagagtg
     9841 atgaagaaaa agaaagtatt gaagaaattc ttgaaaaaat agatgtagat agtccggaca
     9901 gtgattatta cagtgaaata gaaaaaaaat taaactatat atatccatat gaatttagta
     9961 caagaaaacc tgctactata tctgttacag aaataaaaaa gatacaaaat aattatgaag
    10021 aagagttaat aaatactata tttgaacaaa aagtaatatt aaaaaagcct ctatttattc
    10081 aaaatgaaga agaaagagaa aaaataagtg gaacagaaag aggtacaata gtccatttgg
    10141 ttatggaggt tttagactta aaaaatgtaa gtagtgtaaa tgatataaaa tcacaaataa
    10201 gaggttttgt ttcaaaaggt ataataacag aaaaacaagc tagtatagta aatccatata
    10261 aaatatataa attttttgcc t
//
LOCUS       DAEPOZ010000134        10277 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.134, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000134 DAEPOZ010000000
VERSION     DAEPOZ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10277
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.134"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(302..2182)
                     /locus_tag="KR440_002475"
     CDS             complement(302..2182)
                     /locus_tag="KR440_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HBH0699513.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSVTCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRVE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTSDKPALK
                     IVTYNNKTVDYENINSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKITKGRKATINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGNGGNNGGGSNSGGSSSGGSSGGGSSSNSTSNVTVKKLKGADRFETA
                     TKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNEKLPQKTVEELK
                     RLNPSKVIVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAVQFIKSNKVSNIYFIGGI
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVVDKIKSLI
                     EK"
     gene            complement(2392..8355)
                     /locus_tag="KR440_002476"
     CDS             complement(2392..8355)
                     /locus_tag="KR440_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HBH0699514.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGN
                     LEVDLNFSLPIKNSESESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNIGTDNIEYTIK
                     KLSASRSEIKNKDENVSYYNIVFNNLPVGKYNIEVSGAGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDEDGNVQQ
                     IPYENKEYMKNLKDKEQQEEVNNEQEEVNQEEVNNKDENKNQTDNNSNNKDENNDKQQ
                     ENSDKQEVNSNTNQDKNNDKENSSMDKPNSDEDTPKEGDNISKDDNSTTSTYSRAALE
                     TYTDVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKISTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSLKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESIDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVVYPAGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEIRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDDLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIDRELKLAQDLYNDRATLTDVTTVNQ
                     EINKSGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLKTGKNVIQLSKLHSLDIECGGSLYIRYPEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELAKYKDAVKGEPYYPGTNIGNSY
                     EYSEKTGVLNTTDIESDKVTLNVPATAVYQGISKGNADLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRLEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKAKFTDSISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDVSTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPEAETRKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDDKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGTMNNADQTRTNGIGKLAEDYVYQSDDPSTSEDETGKIPAGS
                     IIITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNAIDVSNKKVITVSNKVKGNIVRDK"
     regulatory      complement(8479..8564)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(8736..10139)
                     /locus_tag="KR440_002477"
     CDS             complement(8736..10139)
                     /locus_tag="KR440_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HBH0699515.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILIDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALVASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     DNGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKISDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
BASE COUNT         2840 a         1894 c         1161 g         4382 t
ORIGIN      
        1 ttttccatat attatagagt atatatgata tgaatatatc tcgtctatat attagtgttt
       61 tattttatta aatttaaaaa attattttta acttcttttt aaacatattc aaaattgaat
      121 ttatatttat atatataaaa gatatctgct ttatttaaaa ttaaacttaa tatagctcaa
      181 attattatac attttaacct caatattatt aaattgttaa gataatgaaa ataaaataag
      241 aatgcatcta aatatataac tttatatcta aatacattct tattttcgat ttacttaata
      301 tttatttttc aattaaactc ttaatcttat ctactacaga tgaggctatt cctccaccta
      361 cttcaaacaa ctttgatgaa tttttacctt ttaatgatga ttcttgtgat ttatctaacg
      421 tatttgtagc aagtataact ggtgaattgt ttttagctgc taatggtcca actgctaaag
      481 catctataag tttatcttga tttgcaacta caactccatc tagtttagat tgagagtaga
      541 atttatctat tactttggca ttcgtctctt gtctagtttg acctgccacc ctattgtttg
      601 atatatcttt gtttgctatt tttccaactt gttcaatagc cttgttagat attttttcta
      661 ttccaccaat aaagtatata tttgacacct tatttgattt aataaattgt acagcttcat
      721 tatctacacc atctttttgt gtaagtacta ttggagtttt ttctttaccg gccagtgatg
      781 ctattgataa tgaatctgct tctccatttc ctgcccctat atacaatttg cttacattat
      841 tcgtcttatc tatttctttt gctatattaa tagaagtttg atatcttgta tctccaccta
      901 ttctctgtac tgatattttt gaatttacag ctttgatagc ttcaacaact gaatttggca
      961 ttgagttgtt tccacctatc acaattactt ttgatggatt tagccttttc aattcttcta
     1021 cagttttttg aggaagcttt tcatttgatg ataaaagaat cggtgagttt tttacactag
     1081 caagtggtgt agctgtaagc ccatccacca tgcttttatc ttctccattt actattacaa
     1141 ctgtatctga ttttgtccaa ccactttgag atattttggt agctgtctca aatctatcag
     1201 caccttttaa ttttttgact gtgacattag atgtagaatt acttgaactt cctcctccag
     1261 aacttccacc actagacgag ccgcctgaat tacttccacc accattattt ccaccattgc
     1321 cattatctgg tggtaattca tccggttttg ataattcatt ttcaactatg tattctaaac
     1381 tttttggaag tgtaacttca acatatttat ttattgtagc ttttcttccc ttagttattt
     1441 tttgtgctaa tccaagtttt aatattacat ttgaaccact ttcttgtgtt tctttactaa
     1501 caatagaata gtttatccta atattgttta ataaatattc aagtgcttca cttttctcta
     1561 ccttatactt ctcagggtca tttttactag aaccagttcc accaattggt tttacttcac
     1621 tctcattaat aagggtaaat attaatcctt catcagagtt tatattttca taatccactg
     1681 ttttgttgtt atatgttaca atttttaaag ctggtttatc gcttgttcct tttcctaaaa
     1741 ctttatagtc agcatttgag ttactgccag atttttttac agatattctt cctatattca
     1801 atctatttcc gtctagtaaa tcttcatttg atgtaacata tatatctaat gtagttgtat
     1861 tacttgattt gttgtattct actctacaag tagcatttgc atttccactt tttatctgag
     1921 attttatatt atcagaccaa gtaatctttt ctttctgctc attatctact acttcaacct
     1981 caccttgtac ttttatactg gcttgaaatg aatatactat gttttctact gagtccacag
     2041 atatatctat agtatttact ttctcatacg tttttgttcc ttgtaaaact ggagctgcat
     2101 atgcacatgt aacactacca gttacagcta ctgccatagt tgatactgct atagttctta
     2161 taaaagattt tcttttactc attaacaccc gtccttatct atcattaact aagtggataa
     2221 atttacttat ccacctagct tttatattta atttataaat taccctaaac ttatagttac
     2281 atttatatac tttgctttta atatctacta ttttaaaact attctagtac ctaatcatat
     2341 tagatataca atgttagtat tagtattttt acgtttttag agttccacct actatttatc
     2401 tctaacaata ttacccttta ctttattgct tacagttata accttcttat tactgacatc
     2461 aatagcattt cttgtagctt ttctagcttt actattacta tcagtaagac ttattgtctt
     2521 taactcatca taattcttag ctcctacagg tacatacatt gtatcactta caaatctttg
     2581 acctactggc gtatctcctt gaaggtcatt gtatctatat agttcagcct taaccttatc
     2641 acttaaagaa tcaaagtttt cttttggcat atagtaaacc catgtacctt tactgatttc
     2701 acctaattca cctttttctg gaacttctgc aaataaaagt gcttctccag atatagtctt
     2761 attgttttta tctataagta gtggtaaatt aaatgctgga tttccactgt attcacctgt
     2821 aataattatt gaacctgctg gaatcttacc tgtttcatct tcacttgtgc tagggtcatc
     2881 actttgatat acataatctt ctgctaactt tccaattcca ttagtacgag tttggtctgc
     2941 attattcatt gtgccaattt ctatattatc acctggaggt cctattatat ctaattctgc
     3001 aacttctata ttagacttat tgtttgagat tagttttaca tatctagctt ggtatgtcca
     3061 aagtttatca tctttattaa atgtaactct agcttcatta tcttggtctt ttcctccacc
     3121 aactaaatct tgtccatatt caaaagttcc ttgacttaca tcttcccagt tctttttatc
     3181 attacttact tgtatcttgt aattagttaa aggattgtat gttgtgctag tattctttct
     3241 aaagttaaat aatcctttta atgagaactt cctagtttct gcctctggtt ttgtatattt
     3301 tagtcctact ataggtaatg tttctttcat gtctactatt atatatggat tttcttctac
     3361 atttatatca gggtcttttt gagggtcttt gttccactct gctttactta ttatctttcc
     3421 tttatatgta gtacttacat cattatcttt tatattatca atttcaggat tttgtaatgg
     3481 tccatgtgag tcattttcag ttctaacatc accgtcagaa acagtatttg ttatgaatga
     3541 ccatgctgat ttatttattt tcccttcatc tgataatttt acagtgccaa cttcaaattc
     3601 ttctgttgga tttaggtgat aatcatatgc aactacctta tatgtaaatg ctctgttatt
     3661 aagaggagat atactatcag taaatttggc cttactattt ccttttgcat ccacaaatcc
     3721 aactgttctg tattttacct gtgttccatt ttcaccatct aaataatttc catcacttct
     3781 tattatttcg tatcctagta tcttatcttt atccttatct acatctaggt ctatagatat
     3841 tttattagat ttaactagag aaccactttg tattccatca gaaaaactag cacttacttt
     3901 agtatctttt gccatactag ctatattttc atttaaaatc tttcttctag cttcgtcatt
     3961 tacatattgt atctttcttg attctttttc atagttctta ctatccatta tactgtttaa
     4021 ttctggtgta atttctactc cccatgcctt aaagtaatct cttaaatctt tttcaacaac
     4081 acttgatgcc attgctacta gcatttggtc ttttggtaaa ctattaattg ggtcactact
     4141 tgaaagtgct ctgtattttc ttgaaatctt tgcatagaat gtatcattac ttaaatcagc
     4201 atctgagtta ttctttaaca ttgaacccgt aaaatcatca tcataagcta gatgtaattg
     4261 ccataacatt ccaagccttt caaatacatt gcttggtata cctaagctat ttgaagttac
     4321 atgctcatat atcttatcca ttgttgaacc ttcaagtctt gaatgtgtct tatcatcaaa
     4381 ggtctgagca agtaaagcaa gtacattgtt actagtttcg ctatatgtca tctttccaat
     4441 atctgtaaca tgtcctattt catgacctat tccccatcca aataagtttc ctcctgtagc
     4501 ttttccacta tcatctattt ggaaaggtac tccattcata agccctggaa ctgaaccata
     4561 ttctactcct acatgtaatc cagaagcata cataaatgct ccaataaaca ttctttggta
     4621 ttttatgttc atacgagatt ttggagccat atcattttta gttaagttaa attcattatc
     4681 atctacagtt ccattaccat ctaaatcttg tgttttaaat actcctcttt ctgagtaaac
     4741 taaatccatt atttgttccc atgcaagcat actattatat aatctatcta cttgtgtatt
     4801 taaatcagca tttccttttg atattccttg atatacagct gttgcaggaa catttaatgt
     4861 tactttatca ctttcaatat ctgtagtgtt taacacacca gttttttctg aatattcata
     4921 tgaattacca atatttgttc ctggataata tggttctcct tttacagcat ctttatattt
     4981 agctaattct tctatatatg cttttatctt tttcttagat tcagaaactc ccgcaggact
     5041 cttatcattt attgttccat ttagattaag atgtggtatc ttagtagctc ctgctacacg
     5101 tacttttata tctcctccac ttggagtatc ttcagggtat cttatgtata aacttcctcc
     5161 acattctata tctaaactat gaagtttgct taattgaatt acattttttc cagttttaag
     5221 atttatttct ccactcttat atgcaccaga ttctccatag tactgtgtat atatcaactt
     5281 aggagttttt ccacttttac ttccaacata tactgttatt tcatctcctg ctctagcagc
     5341 tactcctaat gcctgccagt cattttgcat gcctaagtta ttggaattta cagtatactg
     5401 tccgttttta tctttatatt ttcctgattt atttatttct tgatttacag tagttacatc
     5461 agtaagtgtg gctctatcat tatataagtc ttgagctaat tttaattctc tatcaataac
     5521 tgtcttatat ggatgatatt ctccactagc tttatctgga gtatttaatc tatcttttaa
     5581 ttcagttatt ttttcttgag ttacatcttc ttttaattca acttgtaggt catccataaa
     5641 tagatttctt acatcatctt ctatactgtc atactcataa aacttaagtt ctgatatatt
     5701 agacttatat gaataagatg aacaaacact tacttgtatc ttagtagcag ttattggttc
     5761 ttttaatcta accattacat acttattatt atttacaggg tctgtctttg tttcaaaagg
     5821 aacatccccc agattgtgta attgaccatt ttcatcccaa tatcgtatct caaagtcatt
     5881 tattgtctta tcagaaacat caaaacgagg tgtagccata aaagtatcca tagtataaga
     5941 tttatcaaaa gttattattg gtcctctatt cgttccctca gctgcattat atgttgggaa
     6001 tgtccaatgt gttgtgaagt ctccatctac tacattgaat ttatcaaatg gatttttatc
     6061 actatattca cttttgtcat atcctgctgg ataaacaaca tctacaatgt gatttgttgg
     6121 tattccaaat ccaccattct ccttaggagt attaattaat ctatagttag gtgttgttgg
     6181 tgcatctatg ctttcagttg tacctatata tgttttagac attccactag ttcccaagtg
     6241 gttagtagct gtcattctaa cttcatatgt tgtgttatcc ttaagacctt ctatttttaa
     6301 acttgtccca cgaataagct tatcattgta ttttatccaa gacccattag agcctttttc
     6361 tttatagtac aagtcaaagt ctttagcttg tttatgattt ttccagctta catcaagaga
     6421 tttaaattta cctttaacat ttataccttc tggtggttct ggttttgtgt ctggaacaac
     6481 ttgaaattct aatatgctat ctgggctaaa ttgtataggt ttatagtttg agtctacatt
     6541 ttctggtact ccatctaagt tttctggcgt tgcatcttta taaccactag accaatctcc
     6601 atttaaagat tgtatgctaa ccctatatag tccatatgct tctatatctc taacttctat
     6661 tgtattttta ctagtttgta attttttagt agataatact tttcccttac tatcaatttt
     6721 atctagtata agttcgtatg ctgatacatt tacttcatta ttccaataaa aagatacttt
     6781 ttcatttcca acagcagtgc tagtcgatat tttatttatt ttaggtatgt tcatctctgg
     6841 ctttggtatt tctttatata cattgtttaa aaattcaact tttgatattt ctgcaagatt
     6901 tctatcgttt gctgttgtag ctgttatttt tatagttatc ttttttactg ctacttgttt
     6961 accaagattt attactatat cgctattttg aacatctgta tatgtttcta atgctgccct
     7021 actatatgta gatgttgtag agttatcatc tttactgata ttatcccctt cttttggagt
     7081 atcttcatct gaattaggtt tatccattga tgaattttct ttatcattat ttttatcttg
     7141 attggtatta gagtttactt cttgtttatc gctattttcc tgttgtttat cattattttc
     7201 atctttatta tttgaattat tatcagtctg atttttattt tcatctttat tatttacttc
     7261 ttcttgattt acttcttctt gttcgttatt tacttcctct tgttgctcct tatcttttaa
     7321 gtttttcata tattctttat tttcataagg aatctgttga acattaccat cttcgtcagt
     7381 aacctcaact attccttttg ctggagcagt accttctgga gcttttatat tgatgacttc
     7441 catattaagt gcttctgata aagctatatc cattactgct ggtttttcct cagatatttc
     7501 atcatttttt gaagatattg taactccatt attttctcca ctaaataaat tttctacatt
     7561 accattaata tctacatcac tatttccttt taattcaacc ttacttgtat ctactatagg
     7621 attggtagat actatacttt ctaggctttt tgcatcttca ctttgtccta aatttttctg
     7681 tacataatct aagtcaacaa tatctacctt tccatctcta tttaaatcaa attctctttt
     7741 atttgaatct atattctcta aaaccttatt ataatcactc ttatctactt taccgtctcc
     7801 atttacatca cccattaaga ataattcata atttaaaggt ttatttttat tatctacact
     7861 tgctctattg cttaataaga ctctttgtga ataacttgat atatcaatgt ctttaatttc
     7921 tttgttctta aatccagctc cagagacttc tatattgtat tttccaacag gtaaattatt
     7981 aaatacaata ttataatatg atacattctc atctttattt tttatttcac ttctacttgc
     8041 agataatttt tttatagtat attctatatt atctgtccca atatttactg ttcctgaatc
     8101 tttttctcca agctttatta ttccactatt gttactatca tcttttaatc gtaactgcat
     8161 attagtcata ctttctgact cgctattttt tattggcagt gaaaaattta aatctacttc
     8221 aagattcccc cttaactttt ctgtaactga tttttcaact gcccttagct tcccctctaa
     8281 tataggttct gcaaaaacat ttagtggtgc actacacata gatactgcta gtacacaaga
     8341 tattgccttt ttcatgaaac aacaatcctc ccttaataaa tctctaattt ttcatgaatt
     8401 taaatactat aagtttcaga caataaaaaa agacactcaa agagaatctt aattataaaa
     8461 taaaaagact ttactctttt tagcaactgg ctgatatggc tttaaaagct ttaatcccta
     8521 tagttttgcg tcattagatt tccctaattt tgccatttaa ttttcactac tttcttaccc
     8581 attttatact atatttaaac tttttaatac aattttttta cattaaatta taaaaaaata
     8641 acatttatat taataaatta atataaatgt tattttccat tataatttaa ttatttatat
     8701 agcttcctta caagcataat tttgtgtata ttttcttatt ctttatatct tccataaaat
     8761 tcatgtgtag catatagatt cttatctcct gttatagcta taccaaatcc cattatttca
     8821 taccctttat gcaacatatt agttccttct tctggatttg tcttccactc attaaaaata
     8881 aagtttccta tatcatctgc atctctattt gtatatacag gtttatctga aattttatat
     8941 ttaattatat tttcaccttg aactgctata tatccaggtt taatctcact ccaatctaag
     9001 tatttcaaaa aagtagaata tgaatttttt ccatttatcg tatgagataa agtcttctta
     9061 tttgccatta aattagacca atcatttgct agaccctcta atctgcttgc tacagataac
     9121 gctcttactc cattatcttt tctatactgc tttgtaagag catatacttt accctttatt
     9181 ctctctttaa atgctctatc atttggattg taatataacc cttgttgatt tggtgaagct
     9241 tgatttatac attgcttgat aacattctga tttatgcctc ctagtacagt caatttgtct
     9301 gcaccactta aaatactctt gttactgcca ttttcaacta aaactacagg agcatttttt
     9361 gctattgtag aggcgaccaa agcgtctgtt aattgcaagc ctttacttag ataaaattct
     9421 ttactatttt tataaaactc ttgtataact atcttattag tttcaaatct atctgtgcca
     9481 cccaatctaa tagaatttgt gctcttaact aattcgtcgc tgattatttc actagaacca
     9541 agcgcataac tctttaagcc tgtcgtgtca aatcccacac tatttccatc tataagtatt
     9601 ataggtactc catctctagc agctactggt gatgcactta tcgcatctgc ttctcctgta
     9661 aatccattgg ctataattac tttatctatt ggcttaattt ctttgatttt ttcagctatt
     9721 tttatacttg tatcaaatct attttcacca aatattcttt gtacaacctt ctttttggat
     9781 aataactgat tctcaacatt tttacttatt gttgattctg agcctattat atacacagtc
     9841 tttgctttgt ttaatctttc catagttgtg tttggaatct gattttgctt tgtcattaaa
     9901 ataggcgcat ttaaggctcc agctaaacca cttgcactta gaccatctgc taagctatta
     9961 tctgagttga ttagtattgc tgtattgtag ttttgcttat ctgctatttt tgctgctgtc
    10021 tcatatctgt cattcccttt tatttgctca actgatgtca acgcatttac cataggagta
    10081 tttaccatta ctaaaaatag tgtaagtcct atagctaata tctttttact attcttcata
    10141 ttattttcta cccccaatgt acttttcata ttttttatta gtactcatac ttatttttaa
    10201 tcaaaagtta caattttgtt accatatttt atatatgata ccatagttat ccatacaact
    10261 caaacaatat acctaaa
//
LOCUS       DAEPOZ010000135        10065 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.135, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000135 DAEPOZ010000000
VERSION     DAEPOZ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10065
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.135"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..423
                     /locus_tag="KR440_002478"
     CDS             <1..423
                     /locus_tag="KR440_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699516.1"
                     /translation="VVLIILSKIVDDNIKEDASIPNVNKETLEYFRKNYKEDIITCAE
                     EDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIENQTITFKNIDDKA
                     PIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            458..1549
                     /locus_tag="KR440_002479"
     CDS             458..1549
                     /locus_tag="KR440_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0699517.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKAGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            1554..1778
                     /locus_tag="KR440_002480"
     CDS             1554..1778
                     /locus_tag="KR440_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699518.1"
                     /translation="MENIKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            1788..2351
                     /locus_tag="KR440_002481"
     CDS             1788..2351
                     /locus_tag="KR440_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699519.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVSKEFKKSKAYSDFITLYNKSVDALKNKATFKKALK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            2312..2941
                     /locus_tag="KR440_002482"
     CDS             2312..2941
                     /locus_tag="KR440_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0699520.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            3118..3870
                     /locus_tag="KR440_002483"
     CDS             3118..3870
                     /locus_tag="KR440_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0699521.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            3883..4368
                     /locus_tag="KR440_002484"
     CDS             3883..4368
                     /locus_tag="KR440_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699522.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYVSKK
                     LNIEKEYIERTLQYFIYENIMVVDGNSINFIYPVSALQTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            4375..4524
                     /locus_tag="KR440_002485"
     CDS             4375..4524
                     /locus_tag="KR440_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699523.1"
                     /translation="MNFFWTKSDFDAWTNEAGLSDDEDIYCLDINEAIVESYKIFKLK
                     QKVLS"
     gene            complement(4660..5481)
                     /gene="yidA"
                     /locus_tag="KR440_002486"
     CDS             complement(4660..5481)
                     /gene="yidA"
                     /locus_tag="KR440_002486"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBH0699524.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMTLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNEAPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            5973..7667
                     /locus_tag="KR440_002487"
     CDS             5973..7667
                     /locus_tag="KR440_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBH0699525.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSHFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGNIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKISKKLGFEDFVEL
                     GYVRMMRSDYREDMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIQNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      7999..8109
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            8213..8584
                     /locus_tag="KR440_002488"
     CDS             8213..8584
                     /locus_tag="KR440_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HBH0699526.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQDNKKTPKWTENVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            8680..9627
                     /gene="trxB"
                     /locus_tag="KR440_002489"
     CDS             8680..9627
                     /gene="trxB"
                     /locus_tag="KR440_002489"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBH0699527.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            9712..10029
                     /locus_tag="KR440_002490"
     CDS             9712..10029
                     /locus_tag="KR440_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HBH0699528.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
BASE COUNT         4006 a         1046 c         1819 g         3194 t
ORIGIN      
        1 gttgtgttaa tcattttatc aaaaatagtt gatgataaca tcaaagaaga tgcttctatt
       61 ccaaatgtga ataaagaaac attagaatat tttagaaaaa attataaaga agacataata
      121 acttgtgctg aagaagattt aaataatgat gggaaaaaag atttagtagt tatatataaa
      181 aaaagcaata attcaaatga aatggtagtc gtagtttctg ataaaaatag ccactatata
      241 acaaagccaa taccagcacc tatagagaat caaactataa cctttaaaaa tattgatgat
      301 aaagcaccaa tagaagtaat agtatcaggg tctaaaaatg gtaatgtagg atatgctatt
      361 tacagagtag agggtaaaaa atttgttgat ttatttggcg aagatatgga caaatgttgc
      421 taaaaataaa aataatacat aaaggtggga aatttatatg aaattatcta agaagattca
      481 agcactaatc ttatttggtg taatgggtac atctatatta acaggatgta gtagtaaacc
      541 aaaagaaaaa gaagaagcaa aagctggaaa agatgattat acaataacag taggttatta
      601 taattgtgac catatgactg cgggaccagt agcagaatcg gcaggaatat ataaagattt
      661 aggtcttaat gtaaaaactg ttggaaatgg taaagttcct gaagctatgg cagcaggaaa
      721 aatggatgca ggttacatag gaactaaggg gttagttgga gcaataccta agggttcacc
      781 aataacaata gcagctaaca accatactgg tggttctgaa tatttaatag tttcaaaaga
      841 tataaaagaa cctaaagatt taataggtaa aaaaattgca acagatatga gtgatttctt
      901 atggacaagt gattatggtc cagaaacagg tctaccaact gacccatcaa aatatgaagt
      961 tgtaaatatg gattcagaca aagataaata tttagctctt aaaactggga aaattcaagc
     1021 ttttacatct tgtgaccctt ggggttctgt agctgaaaat gacggtgcag gaaaaataat
     1081 agcatctact caatataaag aaaaagctaa tggcaaagaa tataactgtt gttcattctc
     1141 attaaacaaa aactttataa aagaacatcc agatttagca aaaaaattgg ttctagcaca
     1201 tacaaaagca atagaatata tatatacaaa tccagctgaa gcagctaaaa tatttgcaaa
     1261 atattataat gtagaagaag aagttgcact tagaactatc tataagaaga ctgttggaga
     1321 aggtagaact cttacttgga aagttacagg tgaagaatat aaaaataatt tacaaatgta
     1381 taaagattta aaggctttag atgatatacc taaatatgaa gatacgattg atacaagctt
     1441 attggactct tgtggagctg atgattttga caaatttata aaagaaaaag ttaacccaga
     1501 gtttcctgaa ggtatgagtt atgaagactg gaaagcaaag gtattataaa taaatggaga
     1561 atataaaaaa tataataata ctttttatca ttattttggg aagtatattt ataatatttt
     1621 ttaattttga agataaagaa gaaataaaag gaaaattaag aataggggta tctgatgata
     1681 cctctggttt tgtaataaat tatatggtaa atagagacta ctttgaaaat ataaaagtaa
     1741 gtgatataat ggatattttt acaattaatg attgctgagc aagtacaatg cagtgggctc
     1801 tgagttcaga ggaaatagat atagcaatca tatgtaaaga tgctgcaaaa caatatgtaa
     1861 atgtaaatag tggatttgaa attgttggta ctgtagttca aaattctgat atatttctat
     1921 taagtgataa taatcctgaa aagataggag tcattcaaaa tagagattac cagtcagaat
     1981 tggttaaaaa atactacaag aatgtggaag tagctccatt attagggact gcactagcat
     2041 atggattaga aagtaattta gtagatggtg ttgtaataga ttgcataaag tcaattgggt
     2101 tagagggtaa aagaaaatct acaactaaat taggagatta tgatacctat gtattagttg
     2161 taagtaaaga gtttaaaaaa agtaaagcat atagtgattt tataacactt tacaataaaa
     2221 gtgttgatgc tttaaagaat aaagctacat ttaagaaagc attaaaagag tacaggaata
     2281 taagaaatat agatatggag gaaataagca attggaaatt aaaatttcta aagataaaag
     2341 cgatagatta actaagaaaa aaactgatgg tagtatggat attttatata aaataatact
     2401 attagttatt atatttttag tctggcattt tgcagcaaaa gatataggga gttctttgtt
     2461 acttccaatg ccagtagatg taataaaagg tttctttttc tgtgtgacag atgcagagac
     2521 agtcactaat ctttttataa cattgcagag agtacttaaa ggttttatgt atgcattatt
     2581 gtttgggctt ccaataggat ttattatggg attttctaaa acttttgaaa gggttttatc
     2641 tccagtagta gattctgtaa ggcaagttcc aataatggca tgggttcctc ttactatagt
     2701 atggtttggt ataggagatg gtccaacaat atttttaata gctttttcag gggttttccc
     2761 tataatttta aatactattc aaggagtaag agcaatatca aaggattatt ataatgcagc
     2821 tagaagtatg ggagcatcgc caattgtgat atttacaaat gtaattgttc ccgcatctct
     2881 tccagatata ctaactggtt caaggattgc tataagtaca ggatggatgt ctgttatctg
     2941 agccgagttt atagctacga gtgccggtct cggttactca atggtacaag ctcaaacatt
     3001 aatgcataca gatgtattaa ttgggcttat gatatttgca gctttaatag gatttataat
     3061 agatagggta ttaaagttta taaataaaat cctttgtaga tggaggtttg cagattaatg
     3121 aagttaagtg taagagatat aaataagaca tttgtaaata atagagtaca tacaaaggtt
     3181 ttagaagata taagtatgga tatagatgat ggacaatttg tctgtctttt aggaccatca
     3241 ggttgtggaa aaacaacttt acttactata ataggtgggt ttcaaaaatc agaacgtgga
     3301 gatgttttta taaatgataa aagagttaaa aaaccgggca tagatagagc ttttatattt
     3361 caaaattatg ctttatttcc ttggaaaact ataagaggta atgttctatt tcctatgaaa
     3421 cagcagaaaa taccaaaaga aaaaagagaa gaaatgctag aagaactttt ggttatgtca
     3481 gacttgaaag gaaaagaaaa actgtttccg catcaattat ctggaggaat gaaacaaaga
     3541 gtggccatga taagagcttt agcatgtaat ccagaggtat tactaatgga tgaaccatta
     3601 ggagctgtag attttcaaat gagacaaaat ttacaggaag agctagaacg aatatggata
     3661 aagaagaaaa taactgcatt aatggtaact catgatgttg atgaagctgt atatatgagt
     3721 gatagagtta tagtaatgtc gagagataaa ggaagaatta ttgatgatat aaatatagat
     3781 attccaagac ctagaattag aggaagccaa aaatatgaag agtacaaaaa caaattaact
     3841 gatactttat caaagtgtta tgaagtttaa agaggagtag acatgagtca aatagaaaaa
     3901 tacaatagtt tggataatac acaacagaaa ataagaatct ctattatgga tatgataata
     3961 gataaaggtt ctagtgttac acttcaagaa gtgactgaat atgtaagtaa aaagttgaat
     4021 atagaaaaag aatatataga aaggacacta caatatttta tttatgaaaa tataatggtt
     4081 gttgatggga atagtataaa ttttatatat cctgtatcag cattgcaaac aaatcataaa
     4141 gtaactttga gagataatag aagtttcagc gcaatgtgtg ctatagatgc tataggaacc
     4201 tcttgcacat tcaaccaaga tataaaaata aactctattt gtagtgctac aggtaaggaa
     4261 atagaagtaa ttataaaaaa tgaaaagatt gagtatgtga ataatccaaa cttaagagtg
     4321 cttcatataa atcttgataa gcatctaaat tgggctgcta gttgctgaaa tataatgaat
     4381 ttcttctgga cgaagagtga ttttgatgct tggacaaacg aagcgggatt atctgatgat
     4441 gaagatatat attgtttaga tattaatgag gcaatagtag aatcatataa aatatttaaa
     4501 ctcaaacaaa aggtactatc atagatatac atatatgaaa aactaaaaat gacctttaga
     4561 taaaaatgat aggtaatatc catactatat aaattagtat agagtaggga tgtgaaaagt
     4621 atttaaatct aaaggtatat ttttagtttt tttagatgat tagatagcct tattaagtat
     4681 atatttattt ataacctttg caactccatt ttcattatta gtatcagtaa cataatctgc
     4741 aatttccttt atactattac gtgcattccc catagccaca ccaagacctg catactttat
     4801 catatgttgg tcattttctt catcgccaac agcaatagca ttttctatag gtatatttag
     4861 attcgtacat aatgttttaa gagcagttcc tttattagta gttttaccta aaaattctag
     4921 ataaaaagga gcagacctta ctatagtata tttattgaat aaatttttag gtagttgctg
     4981 tatgatttct tccaatcttt ctggttcatc aactatcata attttacaga ttttaataga
     5041 ttcatcaatt ttatcttttt gtatatattt gacttctata ttatttaatt tcgcttcatg
     5101 aagtgtatat tttccaggtg cttcatttgg tgtaattaaa tttgtcattg tatttatgtg
     5161 tattttagtc ttatattttt tacaaacgtt attatatata aaatcaaaat catctaaagt
     5221 catttctaca tcatgtatga ttttctcact tttaacttct tgaataattg ctccattaaa
     5281 gcatataaca taatcacctt catctgttaa atttagttga tttagaagtt cttttacacc
     5341 aggaagaggt ctgcctgttg ttataactac ttttgcccca gccctttttg cctcctgaat
     5401 ggactcaaag acttcaggag taatcctttt ttgtgtattt aatattgttc catcaatgtc
     5461 tagtgcgatt aatttgtaca tatcaacctc caataaatta tatactcaaa tttcctgtga
     5521 aaacgttgac acagctagtg ttgaacaaag tttcagtggg aaatatggtt tgttttgtat
     5581 actattatat caagagataa gtaatttaga aagtttaata atattaattt ttgataaaaa
     5641 tattacttat gtgagatgag aatgtaaaaa ctttgtaatc aataattcca aaattcttaa
     5701 ttacaaataa tggtatcgtt tgcattgtta atataaataa atgtatatta tatagttaga
     5761 agtttgaata aatagactga tggtaatttg ttatattagt tagttttgtg ttataaaata
     5821 ttgtaattcc attaatcata tgttattatt caacatgatt ataatataaa gaatatttaa
     5881 tatatataat aatttataaa aaagtattac atagtacatg taaatctata tttataaata
     5941 caataaagta aagttgtaaa ggagtgcttt aaatgaagtt ttcagagttt aagtatgaaa
     6001 gacccaatta taatagtatg aaaaaagagt ttttgagttg tgtagaagat ataaataatt
     6061 ctcgtaatta taaagagcaa caaaaaaata tacataagat taatttatta agaaataaaa
     6121 tagaaacgtt gtcaaatata gcttcgataa gatatagtac agacacattt aataaattct
     6181 acaaagaaga aaaaaattat tgggatgaat atatgccttt gtatgaagaa ttaaattcac
     6241 atttttataa tgctattgta aattcaaaat ttaaatatga tttgataaaa gagtttggag
     6301 aacaattttt cacaatagta gagtattctt taaaaagttt ttctaaagaa ataataagtg
     6361 aattgcagga agaaaataaa ttgtgttcag agtatactag acttttagct tcagctgaga
     6421 taatgtttga tgggaatata agaaatttat ctggtatggg gaaattcatg tattctaaga
     6481 atagaaaaac aagagaatta gcaaacaaag catattataa tttctttgaa gaaaatgaaa
     6541 ctaaatttga tgacatattt gataaattgg ttaaacttag agataagata tctaaaaaac
     6601 taggttttga agattttgta gagcttgggt acgttagaat gatgagaagt gactacagag
     6661 aagatatgat aaaaaatgtt agaaaacaag tattaaaata tgttgttcca atggccaatg
     6721 agctttatga aaaacaagct aaaagaattg gtctagaata tttaagttac atagatgaag
     6781 gcgtggaatt tttaacagga aatgcaagtt taaagggaga ttcaagatac ataattcaaa
     6841 atggtaaaag gatgtattct gaactgtcaa aagaaacaaa tgaatttttt gattttatgt
     6901 tagaaaatga acttatggat ttagaaacga aaaaaggaaa aggagctgga gggtattgta
     6961 cttatatacc agattataag tcaccattca ttttttctaa ttttaatcaa actgctgatg
     7021 atatagatgt gttaacacat gaggcaggac atgcatttca gttgtatatg tctagatgga
     7081 ttgatatgcc agaaattaat tttccaacgc ttgatagttg tgaaatccat tcaatgagta
     7141 tggaatttat aacatggccg tggatggatt tattttttaa agaagataca gataagtaca
     7201 aattcactca cttatcatct tcaattaaat ttatacctta tggagtaatt gttgatgaat
     7261 ttcaacacta tatttacaaa aatcctaatg tagataagtc aaaacgaaaa gaaatctgga
     7321 gatttttaga gaaaaaatac ttacctcata gaaaatatgg tgataattct tttttagaga
     7381 gaggttgttg gtggtttaaa caaggacata tatttaaaaa tccgttttat tatatagatt
     7441 atgtattagc tcaaatttgt gcgttacaat tttggaaaaa aatgattcaa gatagagatg
     7501 caggatggaa agattatata aatatctgta aagttggagg aacaaaatca tttttagata
     7561 ttgtgagtat gggaaatttg tattctccat ttgatgatgg ctgtattgag tctattatag
     7621 gtgatgttaa atcttggttt gatgaaataa atgacagcaa attataaaaa attatgagaa
     7681 ttttattgta taaaagtgaa aaattaaaaa atatataaaa agtgattatt tgttgtgaaa
     7741 atagtcttgt ttttaactaa attgataaaa taaactaaaa aagatttgta cttaaaaaaa
     7801 aaagaaaata caagaaatat gcaatatcaa catttggaaa aaaaagtaaa aatgtataca
     7861 aaatacatat aaaaaaacta ttgcaaattt tatagaatgt ttatatactt aagtcaacag
     7921 agatagaaaa aagctattta tagaaaacaa aggataaatt gcttaatttt aaattttctg
     7981 aaaaaaataa taatccggat gaagagtatg ggagactgct ttataaaaag cgaccgaagg
     8041 gacaagttta atcttactta cccaaaagat tagatgaaat tctcaggtaa aggtgactgt
     8101 acttggacgg ggctctgaaa atgattttaa cagggaagac aaattgggag atttgttttt
     8161 cctgtttttt atttatcaag taataaataa aagtttgaaa gagagtaaaa aaatgataat
     8221 aattacgaat aatccaaaag taaaagaaga agttcaaggc agagaagttt tgtttaaaga
     8281 cacaacttat attggaatct tagaagcaag tagagatttg atacatgaag gttatgaact
     8341 tttatctcat ccactctatg ggagtgtaaa accaaatgaa acaccttaca ggactgttat
     8401 actaaagaaa ggaaatcgtt tggatataaa ctctttaacc ttaattgaag aagcaatcat
     8461 cactgcaagt aaatttcagg ataataaaaa gactccaaaa tggacagaaa atgtccaaga
     8521 cgattttaga gtaattgatt atgatatatt ttataataca atacaaagaa tgcagtatga
     8581 ataaaaaaat ttgttaaaaa gttattgaaa cagcctaaat attaaatcta atttaatatt
     8641 aaataaaatg agaaatttaa ttggggagga taagtcaaaa tggaaaatgt gtatgactta
     8701 gtaataattg gttcaggacc agctgggctt gcagcaggtc tttatggagc aagagcaaag
     8761 ttaaaaactc taatcctaga aaaagacaag actggtggac aaattgtaat aacacatgaa
     8821 attgcaaatt atccagggtc agttccaaat gcaacaggac caagtttaat agctagaatg
     8881 gttgaacagt gtaaagagtt tggagcagaa atgctaagag ataacattgt tgatactgag
     8941 ctagatggag atataaaagt tttaaaaggg gaaaaggcag aatatagagc aaaagcagta
     9001 ataataggaa caggagcaac accaagaaag attggttgtc ctggagaaaa agaacttact
     9061 ggtaaagggg tttcatattg tgcaacttgt gatgcagatt tctttgaaga ttttgaagta
     9121 tttgttgtag gtggaggaga cagtgcatta gaagaagcaa tgtatttaac taaatttgct
     9181 agaaaagtaa caattgtaca tagaagacaa ggatttagat gtgctaagag tgttgaagaa
     9241 aaagcaaaag ctaatccaaa gatagagttt ttattagata cagtaattga agaaatcaaa
     9301 ggtgatggaa tattagaatc agttgtattt aaaaataaag ttactggaga aactcatgaa
     9361 tactttgcag atgaagaaga tggaacaatg ggagtatttg tgttcgttgg attagatgct
     9421 caaactgatt tattcaaagg taaagttgat atggatgaaa aaggttacat aataactgat
     9481 gaagatatga gaactaacat accaggcgta tttgcagcag gagattgtag atctaagact
     9541 ctaagacagg ttgtaactgc aactaatgat ggcgcaattg catctatagt agctgagaaa
     9601 tacattgatg aaaaatttgg gaactagaat ctgtaaatta agaaatagtg tctgtaaatt
     9661 taatgaataa tatttaaaat taaaataaaa atttcaaggg ggaataagaa aatgttagat
     9721 ttagataagg ctacatttga agaagaagtt ttaaatgcag aaggttttgt atttgttgat
     9781 ttttggagtg aaggttgtga gccttgtaaa gctttaatgc cagatgttca taaattagct
     9841 gagacttatg gggataaaat aaaattctgt aagatggata caacaaaagc tagaagatta
     9901 gctataaaac aaaaagtatt aggtcttcca actatggcta tatacaaaga tggagaaaaa
     9961 gtagacgaag ttactaagga tgatgctaca gttcctaata tagaaaacat gattaaaaaa
    10021 tatctttaat atgttgataa agtattaaca ttaaaagaaa aaatc
//
LOCUS       DAEPOZ010000136         9968 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.136, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000136 DAEPOZ010000000
VERSION     DAEPOZ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9968
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.136"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..453)
                     /locus_tag="KR440_002491"
     CDS             complement(<1..453)
                     /locus_tag="KR440_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HBH0699529.1"
                     /translation="MYAPFFIRFLLSTLTLSILIVIILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSIIKNSPNSIINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILR"
     gene            complement(456..842)
                     /locus_tag="KR440_002492"
     CDS             complement(456..842)
                     /locus_tag="KR440_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH0699530.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            1075..1752
                     /locus_tag="KR440_002493"
     CDS             1075..1752
                     /locus_tag="KR440_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH0699531.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            1728..2189
                     /locus_tag="KR440_002494"
     CDS             1728..2189
                     /locus_tag="KR440_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0699532.1"
                     /translation="MGGAGDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            2257..3036
                     /locus_tag="KR440_002495"
     CDS             2257..3036
                     /locus_tag="KR440_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBH0699533.1"
                     /translation="MNSKIYYFSGTGNSLVVSKILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIIKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPSDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(3286..3912)
                     /locus_tag="KR440_002496"
     CDS             complement(3286..3912)
                     /locus_tag="KR440_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HBH0699534.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDNRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIEVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(4055..4312)
                     /locus_tag="KR440_002497"
     CDS             complement(4055..4312)
                     /locus_tag="KR440_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699535.1"
                     /translation="MILNRTELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FSNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            4638..5150
                     /locus_tag="KR440_002498"
     CDS             4638..5150
                     /locus_tag="KR440_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699536.1"
                     /translation="MKKISQEKISTHIQEVPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEETYSGCKIIVGMEFETGCVFVEGSDELNDEITSFKGLDKYDISNYYLVANYI
                     RCLKKYKLID"
     gene            5405..6109
                     /locus_tag="KR440_002499"
     CDS             5405..6109
                     /locus_tag="KR440_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBH0699537.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTDGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            6106..6876
                     /locus_tag="KR440_002500"
     CDS             6106..6876
                     /locus_tag="KR440_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBH0699538.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQSLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            6873..7649
                     /locus_tag="KR440_002501"
     CDS             6873..7649
                     /locus_tag="KR440_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBH0699539.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLCMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSY"
     gene            7704..8396
                     /locus_tag="KR440_002502"
     CDS             7704..8396
                     /locus_tag="KR440_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0699540.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            8415..9839
                     /locus_tag="KR440_002503"
     CDS             8415..9839
                     /locus_tag="KR440_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0699541.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNITLLSQSNEEGLKRTIRGDDMPYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANSRGR
                     FVFAVIIMALFSPFIYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMKGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
BASE COUNT         3575 a         1177 c         1759 g         3457 t
ORIGIN      
        1 tcttaaaatt tgtattgatt ttttcatatt ttttatttta atattgcaat gaatagtaat
       61 agctatcatg attaaaattc caattatcca tgatattagt aataaaatat ttaaactatc
      121 aaaattaact tggtttaggg atactgaaaa atcgtttaat gcatttgagt tattaattat
      181 agaatttgga ctatttttta taatagaagt cctattagta ctgcttccaa gttgattaaa
      241 agtatataaa tagttaaata catttccaag attaatatat ttaagaggga caaatggaat
      301 tattaacatt ataagcaata taaatccaat attgtattgg tactttgcag aaatatgttt
      361 tttaaatact tttttggcaa gtaaaattat aacaattaat attgataagg ttaatgtact
      421 gagtaaaaat cgtataaaga atggggcata catatttatt tatccccctt agtcgtcctt
      481 ttatttaaga tttctctcag ttcctcaata tcatcttcag atagtttatc attttctaaa
      541 aagtttagta ccatagaatt aagagtacca ttataaaaac gatttaaaaa agaactactt
      601 tctgtagcta cgtattcttc ttctttaaca agtggagtat aaacgaatac tctgctattt
      661 ttttcatatg ttaaagctcc ttttttaact aatctcaata acattgtttg aattgtttta
      721 ggactccatg tacttgtttc taccaacttt tcaattactt cagtagtgct gataggagca
      781 tatttccaaa taattttcat aacttgatat tctgcttctg aaatatgtgg taactttttc
      841 atactatacc tccttctact tacatttgta atacataaag tatattatca ttaatttagt
      901 ttgtcaatta ttaaaaataa ttcatttata gttgattaaa taagcacatg tagttaaata
      961 aaacttttat ttaagctgtg taagtatagc tttaaataaa tggtttatat tgacagctaa
     1021 aaatatagtt gatatactta ttgttgatat aacaactaaa aaggagatat agaaatgata
     1081 attacagtta taaatggaag ccctagaaaa aatggagcta catcaaaagt tttaacttac
     1141 ttgtataaag atatagaaag attaatacca gatgtaaaaa ttaattattt tgatttaagt
     1201 gaggtaaatc catcatattg tattggttgt ttaaattgtt ataaaatggg caagtgtatc
     1261 aatcaaaatg ataaagtaga gtatattcat gatataatta ctaagagtga tggtgttata
     1321 tttggtagcc caacttatgg aagtagtgta actggacttt tcaaggtgtt tacagataga
     1381 gcacatatga tgcttgaaag attattgtat cgtaagccat gtatagcagt tactacatat
     1441 gaaaatgcac gtggttcaaa agcaatttct tttataaaat ccatggtctt agattcggga
     1501 ggttatgttt gtggtagttt atctataaaa actggattta atcaaaatcc tataactgaa
     1561 aaagtagaat caaagataca aaaggtatct aaaaaattta tttattgcat agaggaaaag
     1621 aaaaatccac ctgtgctttc tcaaatctat aattttattg ctataaatgc tgttttaaag
     1681 ccaatggctt ttaaagatat tgagcaatat aagggaataa ttgatagatg ggaggagcag
     1741 gggattattt aaatggaggt aaaatgaaag attttgagaa actatatgaa gctacaacta
     1801 agttagtaag tacttcgtta aaagtaaaag atgatttaaa aaagatgttt aaacaaaatg
     1861 gatatgatat aacaactgac cattatgcgt tattaaggtt tctatgggag caagatggaa
     1921 tttctcagat agatttatgt gaaaaatcat gtaaggataa atctaataca acaagaattt
     1981 tagacgttat gaaaaataag ggattaattg tccgtaaagt agatgttaaa gatagacgta
     2041 agtttcaaat attcctaaca gatttaggta gagaattaga agaaccatta aatgaaattg
     2101 caagtatata tgcaacagag acatttaaat ctatctctga tgaagaattg cctttgttta
     2161 ctgatatttt ggatagaatt ggaaagtaaa tataaatcct attttaagag actttttaac
     2221 tattgatgat gtagaaatat agaaagaggg ataagaatga atagtaagat ttattatttt
     2281 tctggaacag gaaattcgct agtagtttca aagattttaa aagaaacgtt aaatggaagt
     2341 aaagaagtta tacctttagc tatacataga aaagaaaata atatagacat aaatgaagat
     2401 atattggtaa ttgtttttcc tgtttatttt gctgatgttc ctgatataat aaagtctttt
     2461 gtagagaaat taaattttaa tattaatact catatatatg ccattgccac ttgtaatggg
     2521 gtagctggac atagtttatt tactatagat aagttactta aaaaaagggg gaaaaaatta
     2581 tctgctggtt ttttaatcaa tatgcctgga aatgctttga taacaccttc agatgttgaa
     2641 gaagaaagat tgaaaaatca gactaatagg gtagctgaga ttgcaaaatg tatcaataac
     2701 ttggagattg gaaatataga aggaaaaaac aatttaaagt cacatataga tagtgttgta
     2761 cttcgtttag taggtaagaa tatgcaaatt gcaactacta aattttttgc agaaaataaa
     2821 tgtaatggat gtgggacatg taaaagggtt tgtcctgtag agaatataag tatagttgat
     2881 aaaaagccaa agtggggtaa tgagtgtgaa agatgcctag cttgttttca ctggtgtcca
     2941 aaagaggcta ttaatgtaaa aaaatctttt ttaacgaata ggaagaagta tcaccatcca
     3001 gatattactg taaaagatat atttttagat aaatagatat atttgacttg tattaataat
     3061 tgttttatta aataaaaacg ttagagatat aaaagaaata taaagtaatc ttgtaaagag
     3121 taatgttacc tagactaaat ggaatagaga ttaaaaagaa caatataaat aataaattat
     3181 gttaatagta tatagttaca ctaatataaa ataaaagtgc ctcataaata gaagtttttg
     3241 aggcacttat tatttggcta aattagtttt tattattttg taatatcaac cgtttataat
     3301 ctcgaacatt ttttttatat cttctgatgc catttcaaat tttgattttt caatcagtat
     3361 gtttccacca gtcataatat atgatggaaa tactcttaaa ttatcataat aaagttcatt
     3421 attacgaagt ttatatgttg aaatggaagg gatgacattc ttttttatat gctttttagt
     3481 aatcatatta aaagattttt ttgcaacatc tcccattaac attactacct ctaggttttt
     3541 aaataattta atttcttctt ctaaaatagg catatgtaat ttaattgtat cacgagttat
     3601 tgtatattct gatttaggaa gttttactgc attagttata tagataccca tatctaggat
     3661 atcatttatg ttagatactt ctattcctgc actgtcaaat aaaggaatag tagtttttaa
     3721 ataatcagca tctttatcta cactataaaa atcgtcattg gtgttagttg ggacaacctc
     3781 attaatcatt atagttttta ttttgtctgt ttctattgtt atattaggaa ggtaggatat
     3841 tattctgttg tcatcaaaaa gattgttctg tttaataaat tcacttaact ttgattttat
     3901 atttattttc atataacgag tctccttagt tttaagcttt aaattactat aaaaagctag
     3961 ttttaaagca cacaattttg aaaatatagt agcatatttg aaagtataat aacatataaa
     4021 acatgacaac agaatgtcat gttttataaa taatctatcg caacttagaa tttattagag
     4081 actctgcctg ttcataatcg tctttatgaa cataaattaa gtactcataa gagaaatctt
     4141 ccttttgacc taaattgcca agtgttattt cattagaaaa aggtttttta gagtaagcat
     4201 tatttttcac tttatactta tatttaattt tatttactga taatatattg catatctcgt
     4261 taaatctatt taaagaatat cctcggaaaa gctcagttct atttaaaatc ataaggaaaa
     4321 cctcctttta aaagtaatat attgaaaagc ataggagaaa tgtactataa gttgttataa
     4381 ataaaaggta gcaaatttaa attgatttgt aaagttttta agagtattat attagtgata
     4441 aaataaataa tttttataat ctaactaata acaatttaaa ataggtaact tatgtaagaa
     4501 tcgtagggac tacggagata tcctgtagag aattaataag acatatttaa gtatgcaaaa
     4561 ttctattaag taggaacctt gtaactttag tcatgtgaga tttataagga tatttaagta
     4621 ttgaaaggag agtttaattg aaaaaaatat ctcaagaaaa aataagtaca catatccaag
     4681 aagtaccaca ggaaaaaatt gaacagtgga aatctttata tgaaaaatat aaaagtattt
     4741 taaaagtaaa ttctaagtct gttttagaaa taatagagtt tttaaagcga aaatatgtaa
     4801 tagtagaaga aacttcagag gaatttaaac atgtagttat caataatatt aagttaaaca
     4861 agcctttttc acaaaagatt tccagtgata aagaattaaa acctattgta tttacaattc
     4921 caaatgaagg aaatgcaaaa aaattgtacg atactcaaga ggaaacttat agtggctgta
     4981 agattattgt aggtatggaa tttgaaacag gttgtgtttt tgtagaaggt agtgatgagc
     5041 ttaatgatga aataacttct tttaaaggat tagataaata tgatataagt aattattatt
     5101 tagtagcaaa ctatattaga tgtctaaaga aatataaatt aatagattag tgtttgatta
     5161 ataaattagt gcttgattta aggattatag agtaaatttg aagttagtgt gataatgaaa
     5221 ctaagcttaa atctaatata actgtaaaac ataaacttaa atagagttgc caaaataaaa
     5281 ttggtaactc tattttttat tttaattttt attaaaatta gtaggtgtta tagaattgtt
     5341 attttttcat gttagtattc tcttagtaat agatttaagg tattacaaga agggagaaat
     5401 taatatgaaa gatttaattc ttgaaacaaa aaacttatca aaaagatatg gagaacaaat
     5461 ggcagttaac aatgtttctc tacaaattga acgaaatagt atatatggac ttttaggacc
     5521 aaatggagca ggaaagtcca caacacttaa aatgcttgtt ggtcttcttc gcccaacaga
     5581 tggtcgtatt atttttgata gaaagccttg gaaacgagaa tctcttgcaa aaataggttc
     5641 tttaatagaa tcccctgcat tgtatggaaa ccttacagca gaggaaaatt tgttagtaca
     5701 tacaaaaata ttgggaatac ctaaagataa aatttatgaa gtgttggaaa tagtaaattt
     5761 gaaaaataca ggtaaaaaga gagcttcaca attttctatg ggtatgaaac aaaggttagg
     5821 aatagctatt gcacttttga gtgacccaga gttgttaata ctagatgaac caaccaatgg
     5881 tcttgacccc tttggcattc aagaattgcg tgaattgatt gcttcttttc caaaaaaagg
     5941 tataactgta attctttcta gccatattct atcagaggtt gctcaagttg tagataatat
     6001 aggaattata aatggaggac gactactatt tcaaggaatg cctgacccta atgaaaatct
     6061 agaagaattt tttacagatg ttattcttaa aggaggacaa aacagatgaa tgctttacaa
     6121 tcagaacttt taaagtataa aagaacattt atgggtaaac ttattgtttt tttcccagtg
     6181 tcttttgcag catatgcatt tataatgcaa tcaacactca tgcaaaatcc attatctcaa
     6241 acaacttctt gggcgtggca aagtctttta gcattaattt ttaactggtg gtcattttta
     6301 tttctgccta ttggatttgc attgtttgct actttggtag ctttccagga aaaaaaagct
     6361 ggaaattatc gtgctttacg tacacacaat gtgtctccta tgacactttg gattaataaa
     6421 gttatagcta tggcagtcta cagttttatt tcaacgttag ttttaatagt cgtaacaatt
     6481 ataactggtt taatattgaa agctggtcca gttccttttg gacagattat aggggcaagt
     6541 attgtatgct gggttgtttc tcttgctata cttccacttc agctatggct agctacatgg
     6601 aaaggtatgt tcctaagtat gggagttgga gctttaggta tgatatttgg tgtccttgct
     6661 gcgacaaaac cattttggat tgcagtacct tggagttggg cagttagaat ggtatgccca
     6721 atcatcaaca tacatcctaa cggtactata ttagaagctg gagacccact tttgaatact
     6781 tcagtgattc ctattgggat tgttgtttct ttggttgtgt ttatagtatt aacagcgctt
     6841 actgcagctt ggttcaatag gagggatgac aaatgataaa gctattatca tcagaatggc
     6901 ttcgagcaaa gaggacagct gtccagtggc ttactctttg tatgcctatt attttttcat
     6961 tatgtgtggt tttttatctc acaactaaat ctggaagtac acaagaattt gcttttgaag
     7021 gattttttac agtatggact gtattcatca ttcctattgg agtaggtatt ttagcaggat
     7081 ttatagtgca ggaggaagag cttgctggaa attttaatgg ttttttatgt gtaggtattt
     7141 ctcgtgttag attatatctg ggaaaatttc tttttcttct gttttgccta actatatgca
     7201 catttattgc tacgttgata ctttgcatag gaatgagtat tgcagtgcca tctggagcaa
     7261 caatatggtt attcctgtca gcagcaggac ttgtggtgat tgggacactt ccactattag
     7321 ctattcatct ttgggtgagt tttgcatggg gtatgggtgc atctattggt attagtatcg
     7381 gtggaatttt gatggcagca atacttggat taactagcgt tggtgaaaaa gtatgggcat
     7441 ttgttccatg gacatggcca gtaaaactag gtatgcttcc aggaatttat tttattaaag
     7501 aagctggaac tatttcaact gaggcatttt actctaaagt gatgcaaaca gctagtatag
     7561 agcttatagt agtagcaata ggtttgatta tattcttgat aggaggcgtt atttggttta
     7621 aaatgtggga gggaaggaaa agttattagt agacgaatat tgttttaata ctgaccttcc
     7681 cataagagaa aggtggtgtt atattgagta aaatattgat tattgatgat gaaatggacc
     7741 tagtaatgct acttgaggat gaattgaagg caaaaggaca tgaagtgttg atagcttatg
     7801 atggtcaggc aggtatagag ttatcaaaac aggagcctga ccttattata ctggatatta
     7861 tgatgcctaa aatgaatgga tttgaggtat gtcaggcaat tagagatgat gttttatgcc
     7921 caattatatt tttaagtgca aagcaatcag aaaccgacaa aatcaaaggg ttgacctttg
     7981 gaggggatga ttatgtaaca aagccatttg gactgcgtga acttatggca aggattgaag
     8041 ctaatctgcg tagggaaaag cgttcacagt atatcaatga agagaacaag agatctactt
     8101 tgtattttgg gaagttatgc ttatatatga aggaacgtgc aattaaaatt gatggtaaga
     8161 atattgattt aacaaaaata gagtatgata ttgttgaatt actagcactc catgcaggac
     8221 aagttttctc aagagaacaa atctatgaaa aagtatgggg gtgtgattct gaaggtgatt
     8281 cctcaactgt tgttgaacga gtaaaaaaaa tacgagctaa gttttctgct gtgacaccag
     8341 aaaaagaata tatttccaca gtttggggta ttgggtataa gtggaataaa atgtaaagag
     8401 agagaggtta ttaaatgaaa aatcaatccc ttattaccca atttagacat acttttattt
     8461 ttattataat agccagtata gttgccacag tgattactta tgtatttgct ctctatttgt
     8521 atattcattc cttaaataag gatatctatc caccaaacta ttatgagcga caagttccta
     8581 gaattgaaaa atacatcaat gaaaaaaaca taactttgtt atcacaatcg aatgaagaag
     8641 gtttaaaaag aacgattcgt ggggatgata tgccatatca agttgtggat aataatggca
     8701 atattctata tggtactaac cccaaaaaac tatttaaaac aaaagaagaa ttatttaata
     8761 attttatcaa taaaactgtt cgtaaaggtg gttatatcca tactgttcca atcaaagggg
     8821 ataatggaaa gattgaggga gctgttattc tattctatca agtcaaaata acctttgcaa
     8881 atagtagagg gagatttgta tttgcagtta ttataatggc tctgttttct ccatttatat
     8941 atatcgtggg ttttactaga tggctttcta agcgctttgt aaagaatatc aatcaaccat
     9001 tacatctatt gattgatgcg tccaagaaga taaaagaaaa ggaccttgat tttgaaatcg
     9061 actattattc agataatgaa ctcggtaaac tttgtagtgc cttttctgaa atgaaagacg
     9121 aattaaaagg ttctttatct gcgcagtgga agatggagca ggaacgtgtt gaaatggtgg
     9181 aagctctagc acatgattta aaatctcctc tttctattat tttgggatat acagatgcac
     9241 ttattggtaa taatacagat gacaatgaaa aacttcatag gtatcttaca gtgataagag
     9301 aaaatacaga aaaaagtgct gctcttgtcc aaaagatgca atatacgtct gacttagaaa
     9361 agtcaaatat acaattaaat cttgttccta taaatttacc tgaatttttg aggcaaaagg
     9421 tgcaagacta tgaattacag gctcatcaaa aggaagttga acttatattg aaaatgaagg
     9481 ggaatataca atccccaatt caaatcgacg tggataggtt gacacgaatt tttgataata
     9541 tcatatccaa cagtctgcaa tacactccat caggtggtaa tattagtatt actgtaaagg
     9601 atgaaaaaaa ttgtatttct tatgagattt gtgattctgg tagagggttt agctctaaag
     9661 atttaaaaaa ggctcttgat aagttttatc gaggagatga agctaggcag acaaaaggtg
     9721 gacattctgg tcttgggctt tacattgtca aacaattagt ggaacagctt ggaggttctg
     9781 ttaaaataga aaattcaaaa tcaggtggag catgtgtgaa attttggcat agcatttaac
     9841 tatatatttt aaattaaagg ggagtgtctc aaagcgaaat ttttaattcg tgaggcactc
     9901 tttttttgtg aagttaaata tattttcatt attccgacaa tgaaaaaagg cttatctcta
     9961 ttttgaga
//
LOCUS       DAEPOZ010000137         9743 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.137, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000137 DAEPOZ010000000
VERSION     DAEPOZ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9743
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.137"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            116..1519
                     /locus_tag="KR440_002504"
     CDS             116..1519
                     /locus_tag="KR440_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH0699542.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFMLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     TSLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     misc_binding    1610..1850
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1893..2591
                     /locus_tag="KR440_002505"
     CDS             1893..2591
                     /locus_tag="KR440_002505"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBH0699543.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILKGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            2614..3615
                     /gene="trpS"
                     /locus_tag="KR440_002506"
     CDS             2614..3615
                     /gene="trpS"
                     /locus_tag="KR440_002506"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBH0699544.1"
                     /translation="MSEKKVIFSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANAIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
     gene            complement(4267..6897)
                     /gene="cdtB"
                     /locus_tag="KR440_002507"
     CDS             complement(4267..6897)
                     /gene="cdtB"
                     /locus_tag="KR440_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094607992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylating binary toxin binding subunit
                     CdtB"
                     /protein_id="HBH0699545.1"
                     /translation="MKIQMRNKKVLSFLTLTAIVSQALVYPVYAQTSTSNHSNKKKEI
                     VNEDILPNNGLMGYYFTDEHFKDLKLMAPIKDGNLKFEEKKVDKLLDKDKSDVKSIRW
                     TGRIIPSKDGEYTLSTDRDDVLMQVNTESTISNTLKVNMKKGKEYKVRIELQDKNLGS
                     IDNLSSPNLYWELDGMKKIIPEENLFLRDYSNIEKDDPFIPNNNFFDPKLMSDWEDED
                     LDTDNDNIPDSYERNGYTIKDLIAVKWEDSFAEQGYKKYVSNYLESNTAGDPYTDYEK
                     ASGSFDKAIKTEARDPLVAAYPIVGVGMEKLIISTNEHASTDQGKTVSRATTNSKTES
                     NTAGVSVNVGYQNGFTANVTTNYSHTTDNSTAVQDSNGESWNTGLSINKGESAYINAN
                     VRYYNTGTAPMYKVTPTTNLVLDGDTLSTIKAQENQIGNNLSPGDTYPKKGLSPLALN
                     TMDQFSSRLIPINYDQLKKLDAGKQIKLETTQVSGNFGTKNSSGQIVTEGNSWSDYIS
                     QIDSISASIILDTENESYERRVTAKNLQDPEDKTPELTIGEAIEKAFGATKKDGLLYF
                     NDIPIDESCVELIFDDNTANKIKDSLKTLSDKKIYNVKLERGMNILIKTPTYFTNFDD
                     YNNYPSTWSNVNTTNQDGLQGSANKLNGETKIKIPMSELKPYKRYVFSGYSKDPLTSN
                     SIIVKIKAKEEKTDYLVPEQGYTKFSYEFETTEKDSSNIEITLIGSGTTYLDNLSITE
                     LNSTPEILDEPEVKIPTDQEIMDAHKIYFADLNFNPSTGNTYINGMYFAPTQTNKEAL
                     DYIQKYRVEATLQYSGFKDIGTKDKEMRNYLGDPNQPKTNYVNLRSYFTGGENIMTYK
                     KLRIYAITPDDRELLVLSVD"
     gene            complement(6950..8341)
                     /gene="cdtA"
                     /locus_tag="KR440_002508"
     CDS             complement(6950..8341)
                     /gene="cdtA"
                     /locus_tag="KR440_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylating binary toxin enzymatic subunit
                     CdtA"
                     /protein_id="HBH0699546.1"
                     /translation="MKKFRKHKRISNCISILLILYLTLGGLLPNNIYAQDLQSYSEKV
                     CNTTYKAPIERPEDFLKDKEKAKEWERKEAERIEQKLERSEKEALESYKKDSVEISKY
                     SQTRNYFYDYQIEANSREKEYKELRNAISKNKIDKPMYVYYFESPEKFAFNKVIRTEN
                     QNEISLEKFNEFKETIQNKLFKQDGFKDISLYEPGKGDEKPTPLLMHLKLPRNTGMLP
                     YTNTNNVSTLIEQGYSIKIDKIVRIVIDGKHYIKAEASVVSSLDFKDDVSKGDSWGKA
                     NYNDWSNKLTPNELADVNDYMRGGYTAINNYLISNGPVNNPNPELDSKITNIENALKR
                     EPIPTNLTVYRRSGPQEFGLTLTSPEYDFNKLENIDAFKSKWEGQALSYPNFISTSIG
                     SVNMSAFAKRKIVLRITIPKGSPGAYLSAIPGYAGEYEVLLNHGSKFKINKIDSYKDG
                     TITKLIVDATLIP"
     gene            complement(8731..9477)
                     /gene="cdtR"
                     /locus_tag="KR440_002509"
     CDS             complement(8731..9477)
                     /gene="cdtR"
                     /locus_tag="KR440_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HBH0699547.1"
                     /translation="MDILIFDNDVCFGIKLKEKINNILIKEGFDNDVIRLYYNANLLL
                     KELTEKNKVKIYFIVVDAKYKISNELCDGLWIAQKIRESDYISPIIFLTNHIEMILGI
                     FDYRLEVMDFILKHDMEIAESKIKACIKIAHKRYVKEKNYRSNFFTIYSDSSLWKISF
                     DEVIYFETSAIPHKIKLVTTSRIFEFYKSLRSLSDLDACFIRVHKSFVVNKYHIVSLD
                     LKKNNIKMSNGHICRISNTYRNILKNIIKT"
BASE COUNT         3360 a         1276 c         1396 g         3711 t
ORIGIN      
        1 ctgtttttga atatgcaaat attttgaaaa atatcagttt aataataatg aatctatttt
       61 attaattttg aaattaaatt taaaataatt aatagagagg ggaaatgaaa ttaggatgga
      121 aaagaaaaaa ataggcctat ggagtttggt atttatgaat gtatcagctt tatatggtat
      181 aagatggata gctaagtcta cagcatcaag ctttggttta ggattaggtg caatacctat
      241 gtggttatta ttctcattta tatactttgt accaggagct ttaatttgcg ctgaattagc
      301 ttctacatat caaaagggtg atggaggtct ttacgattgg gttaaggaag cctttggaga
      361 aaagtatgga tttttagttt cttggttaaa ctggacagct aaaatatttt ggtattcttc
      421 atttttaact ttcttagcag taaatgtttc ttatacaata ggaaatccag ctcttgctga
      481 taataagatg tttgtactta tattatcttt aataatattc tgggcattat ctcttatagc
      541 aactaaagga ataagtttta caaaaatatt tactaataca ggtgcattag gttcaacaat
      601 accaagtgct atacttataa tattttcatt tgtagcagta tttatgttga aaaaacatga
      661 tatagcttct acatatacaa tagcttcaat gactccaaaa cttaatatgg atgcatttgt
      721 tgctatatca gctataatgt ttggtttagc aggagcagag actgctgcta acttcataac
      781 agaaatagat aagcctgaaa agaacttccc taaggcaata ttaatatcag ctggaatagt
      841 agcttcatta tatgtattag gttcaattgc aataacaatg ataataccac cagaccaaat
      901 tactgcttct aaaggtgtat tagatgcact ttctgctgtt tgtacgtctt taggaattgg
      961 ttcttggttg gttcaattaa tagcttttgg tatagctttc tcagtattag gagcaatagt
     1021 tctttatata gcatcaccaa taaaaatgtt atttggtagt gttaaaaaag gaatatttcc
     1081 agatagtttg gtagaggtaa atgaacacaa gataccttct aaagcagtta tacttcaagc
     1141 aataatagtt actataatac tattggtgac tacattaatg ccttctgttg atgctatata
     1201 caatgttctt gtaactatga cagcattaac tgcattattc ccatatgtat tattatatgc
     1261 ttcttatatt aaactaagaa aagagagacc agatgaaatt agaccttata ctatggcaaa
     1321 atcaacaagt acttgcctag gtttagcaaa aatggtttta gttgtaactg tagttggtat
     1381 attattatct gcagcaccag ttatgccgac atttgctgaa aatgttatat atgagataga
     1441 gatgattggt ggaggtcttc tagttatatt atctggtcta tggttatgga atagatatga
     1501 aaagaaaaca gctaaataaa atgtatttta aaaatttctt ttggatatta taatatatag
     1561 ttgacaattt atagtgtaag gaatattata aaataaagaa attaataaat taaatcctga
     1621 gataagaaat agtaaataaa tatatttttg cacagagaat taaggatgct gagaacttaa
     1681 taaaaagtat ttattgaatg ggtctttgag gagtaaaatg aaatgtaatt taagattaca
     1741 gagtatttga tatcgtgtat gcacgttata gcatatatta agatatatat attcagtttt
     1801 gagtgtatat aaatttaggt ggtaccacgg aaaatatatc cgtcctattg atttaggacg
     1861 gattttttaa tatagaaaat ttaggaggaa aaatgaacgt aataggaatt ataggtgcaa
     1921 tggatgaaga agttagtatt ttagttgatt taatggatat tagagagact ataaaaaaag
     1981 ctagcttaga attttataag ggaattttga aaggtaagaa tgtagtgtta gtaaagtgtg
     2041 gtataggtaa ggttaactct gctctttgtg cccagatact aataagcgaa tttaaggttg
     2101 atgctattgt taatacaggt gttgcaggag ctttaaatga aaaacttgat gttaatgata
     2161 tagtaatatc aactgatgct atacagtatg atgtagatac tacagcattt ggagacccta
     2221 agggagttat accaagaatg aagacttctg tatttaaagc agatgaaaga cttatagatg
     2281 ctgcttacaa atcttctgtg gaagaagtaa agactcataa agtattaaaa ggaagagtag
     2341 ttacaggaga taaatttata aattctaaag aattaaaaga agaattagta aatgattttg
     2401 gtggatattg tggagaaatg gaaggtggag ctatagctca tgtatgctat ttaaataata
     2461 ctccattcgt aataataaga gcaatgtctg ataaggcaga tggaagtgct gatgttactt
     2521 atgatgtatt tgtacatgat gctgccaata actctaaaga catagtttta aatatgttaa
     2581 aatcaatata aatatataag gaggtacaaa aaaatgagtg aaaaaaaagt tatatttagt
     2641 ggagcacagc catcaggcaa aatgacttta ggaaattatt taggagctat aaaaaactgg
     2701 actgaacttc aagataatta tgattgctat tatagtatag ttgatttaca tgcaataaca
     2761 gtacctcagg accctaaagt tttaagagcg aatgctatag aattacttgc tcaatatata
     2821 gcttgtggat tagacccaga aaaaaatact atatttatac aatcacatgt caaagagcat
     2881 gttgaactta tgtgggtatt gaatactatg acttatatgg gtgaattaag tagaatgact
     2941 caattcaaag ataaatcaca aaagagtgaa gcaaatctaa atgcaggttt attcacatat
     3001 ccagttctta tggccgctga catattgttg tatcaaactg acctagttcc agttggagat
     3061 gaccaaaaac aacatttaga attagctcgt gatttagcaa ataggtttaa taatagattt
     3121 tcacctactt ttgtagttcc agaaggatac tacccaaaag gtggagcaag agtgatgagt
     3181 ttgcaagagc caactaaaaa aatgagtaaa tccgattcaa atgaaaatgc atttatacta
     3241 ttagcagatg attcagattc tataaagaga aagataaaaa gaagtgttac tgattcttta
     3301 ggtgttgtaa aatacaatga tgaacaacca ggtattaaaa atctaattga tatatattct
     3361 aatttaagta aaaagagtgt agaagagatt gtaaacatgt atgaaggaaa gggttatggc
     3421 atatttaaag aagatgtggc tgaagtagta tcagaagctt taagacctat aagagaaaaa
     3481 tatgttgacc ttcttaataa taaagattat ttagaaaaaa tatatagtat gggagcagaa
     3541 aaagccgaaa aacaagctag aaaaactctt agaaaagttt ataaaaaagt tggatttata
     3601 gagagaagat actaattatt agctatcttt acttgataaa taaattttaa agttattaaa
     3661 aaggtatatg atatgaaaga gagagtctca aagtgaactt ttgttcatga gacactcttt
     3721 tttgtatgaa ataaaatata ttttaatcac ttcaacaatg aaaaagagga ttatctctat
     3781 tttgagacag catcttttga taataaaaaa agatgagctt ttgctcatct caaaggggtt
     3841 agattattct tactttgggg aaagtagaat aaaggtaaaa gttaactgat gtcaactttg
     3901 tcttttttgc atatcactac tgagcataaa aaaatcagaa aaccatagcc ttctgaaaca
     3961 acttctaaca tacattgtat tttataccca atatgtaatt tatgagttaa ttttaactct
     4021 tataatggaa attgtcaata atatatcaaa aaaatatttt ttaaatttat ataattaaat
     4081 gaatttatgt ttatatatag gtgatatgat acagataatt atctttataa tacaatatag
     4141 ttctaaaaaa gaatatgtta gaagaagaga atatttaaat aaaaaatacc ttataaagtt
     4201 aattttttag tactttataa ggtaaatata tattgtattg tggggacaaa tttaaatcat
     4261 ttgatactaa tcaacactaa gaactaataa ctctctatcg tctggagtaa ttgcatatat
     4321 tcttaatttc ttgtatgtca taatattttc tccacctgta aaataactcc taagattaac
     4381 ataattagtt ttaggctgat ttggatctcc taaataatta cgcatttctt tatctttagt
     4441 tccaatatct ttaaatccag aatattgtaa agtagcttca actctatatt tttggatata
     4501 atcgagagct tctttattag tttgtgttgg tgcaaaatac ataccattta tataagtatt
     4561 tcctgtactt ggattaaaat ttaaatctgc aaaatatatt ttatgtgcat ccattatttc
     4621 ttggtcagtt ggaattttaa cttctggttc atcaagtatt tcaggagtac tatttagctc
     4681 tgtaatagat aagttatcta agtatgttgt accactacca attaatgtta tctctatatt
     4741 agaagaatct ttttcagtag tttcaaattc ataactaaat tttgtatatc cttgttctgg
     4801 taccaaataa tccgttttct cttcttttgc ttttatcttt acaattattg aattagatgt
     4861 taaaggatcc tttgaatatc cactaaaaac ataacgttta taaggtttta gctcagacat
     4921 agggatttta atcttcgtct caccatttaa tttatttgct gagccttgta aaccatcttg
     4981 attcgtagta ttgacattac tccatgtact agggtaatta ttataatcat caaaattagt
     5041 aaagtaagtt ggtgttttta taagtatatt cattcctctt tcaagtttaa cattatatat
     5101 ctttttatca gacaaagttt ttaaactatc tttaatctta ttggctgtat tatcatcaaa
     5161 tatgagttca acacaacttt catctattgg tatatcatta aaatataaca aaccatcttt
     5221 tttagtagcg ccaaaagctt tttcaattgc ttctccaatt gtaagttcag gtgttttatc
     5281 ttctggatcc tgtaaatttt tagcagtaac tcttctttcg taagattcat tctctgtatc
     5341 taatataata gatgcagaaa tactgtcaat ttgacttata tagtctgacc aactatttcc
     5401 ttctgttact atttgtccag aactattttt tgtaccaaaa tttccactta cttgtgttgt
     5461 ttctaattta atttgctttc cagcatctaa tttttttaat tgatcataat ttataggaat
     5521 cagtctagag ctaaattgat ccattgtgtt aagagctaga ggtgaaagcc cttttttggg
     5581 ataagtatct ccaggagata gattattgcc aatttgattt tcttgtgctt tgatagttga
     5641 taatgtatct ccatctaaca ctaaatttgt tgttggtgtc actttgtaca taggtgcagt
     5701 acctgtgttg taatatctaa catttgcatt tatatatgca gattctcctt tgtttatact
     5761 taatccagta ttccatgatt ctccattact atcttgaaca gcagttgaat tatctgttgt
     5821 atgggaataa tttgtagtta cattagctgt gaatccattt tgatatccta cattaacaga
     5881 cacaccagct gtattagatt cagttttact gttagtagta gctctggaaa cagttttacc
     5941 ttgatcagta gaggcatgtt catttgtaga tataattaat ttttccatac ctactccaac
     6001 aattggatat gctgcaacta acggatctct tgcttcagtc tttatagcct tgtcaaaaga
     6061 acctgaagct ttttcataat ctgtatatgg atctccagca gtatttgact ctaaataatt
     6121 tgatacatat ttcttatagc cttgttctgc aaaactatct tcccacttaa ctgcaattaa
     6181 gtccttaata gtatatccat ttcgttcata tgaatctggt atattatcat tatctgtatc
     6241 caaatcttcg tcttcccaat cagacatcaa ctttgggtca aagaaattgt tatttgggat
     6301 aaatggatca tctttttcta tattagaata atctcttaag aataaatttt cttctggtat
     6361 aattttcttc ataccatcta attcccaata aagattaggt gatgataaat tatctattga
     6421 acctaaattt ttatcttgta gctctattct aactttatat tctttaccct ttttcatatt
     6481 aactttaagt gtatttgata tagtactctc agtatttact tgcattaaga catcatctct
     6541 atcagttgat aatgtatatt caccatcctt agaaggaatt attcttcctg tccatcgtat
     6601 agattttaca tctgatttgt ctttatccag aagtttatct actttctttt cttcaaattt
     6661 taaattacca tcttttatgg gtgccattaa ttttaaatct ttaaagtgct catctgtgaa
     6721 ataatatccc attaatccat tgtttgggag tatatcttca tttacaattt cttttttctt
     6781 attagaatga ttacttgtac tagtttgagc atatacagga tatactagtg cttgactaac
     6841 tatagctgta agtgttaaaa aacttaatac ctttttattc ctcatttgta ttttcattgt
     6901 ttctcctcct taaatttgaa aaatattaaa ttatttatgt gaaaaaatat taaggtatca
     6961 atgttgcatc aacaattaat tttgttatag taccatcttt gtaagaatca attttattga
     7021 ttttaaattt gcttccatga tttaaaagca cttcatattc acctgcataa cctggaatag
     7081 ctgatagata agctccagga gaacctttag gtatagttat acgtagtact atttttcttt
     7141 tagcaaatgc actcatattc acactaccaa tactagtact aataaagttt ggataagaca
     7201 gtgcttgtcc ttcccatttt gatttaaaag catctatatt ttctagtttg ttaaaatcat
     7261 attcagggga agtaagagtt aaaccaaatt cttgaggacc agatcttcta tatacagtta
     7321 aattagttgg aataggttca cgttttaatg cattttcaat gtttgtgatt ttagaatcta
     7381 attctgggtt aggattattt actggaccat ttgatattaa ataattatta attgcagtat
     7441 atcctccacg catataatca tttacatcag caagttcatt aggtgttaat ttattactcc
     7501 aatcattata atttgcttta ccccaagaat cccccttact tacatcatct ttaaaatcaa
     7561 gactacttac aacagatgct tctgctttaa tatagtgctt cccatctata actatacgaa
     7621 caattttatc tatttttata ctatatcctt gctctattaa tgtacttaca ttgttagtat
     7681 ttgtatatgg taacatacca gtatttctag gtaattttaa gtgcataagt aatggtgtag
     7741 gtttttcatc accttttcca ggttcatata aagaaatatc tttaaatcca tcttgcttaa
     7801 ataatttgtt ttgtatagtt tctttaaact cattaaattt ttctaatgaa atttcgtttt
     7861 gattttctgt tcttattact ttattaaatg caaatttttc tggagattca aaataataga
     7921 catacatagg tttatctatt ttattttttg atatagcatt tcgaagttct ttatattctt
     7981 tttctcgaga atttgcttct atttgataat cataaaaata atttcttgtc tgagaatatt
     8041 tacttatttc tacagaatct tttttatatg attctaatgc ttctttttca gatctttcaa
     8101 gtttttgctc tattctttct gcttcttttc tttcccattc tttagccttt tctttatctt
     8161 taagaaaatc ttctggtctt tctataggag ccttgtaagt agtattgcaa actttttcac
     8221 tatagctttg taagtcttgt gcataaatgt tattaggtaa caaaccacct aaagttagat
     8281 ataatatcaa caatatagat atacaattac taatcctttt atgtttccta aattttttca
     8341 tttattctcc ctcccaatat tagtttaatt aactcttgaa ctttaattta tttagattat
     8401 ataatctaaa taagaatttt ttatataaat ttcataaaat atgtaactat atatcataaa
     8461 aatatgtatt tatatgatat gtcagtacaa aataacaact gaatatttta aagagactat
     8521 ttcatacttt tatgatttaa tgagctatct tgttgaatca taatctagga acaatggttg
     8581 caatgtctaa tagaaattgt atatgatata gtcattatgt gttttttcac aaaacgatta
     8641 ttatatagag gattcttagg aactactttt tttgtataaa aaaagagatg gttcaaattc
     8701 ttatcttgag acatctcttt tttctattta ttatgtttta ataatgttct ttaaaatatt
     8761 tctatatgta ttagatatac gacatatatg gccattactc attttaatat tatttttctt
     8821 taggtcgaga gaaacaatat gatatttatt aactacaaaa gatttatgta cacgaataaa
     8881 gcatgcatct aaatctgata aagaccttaa acttttatag aattcaaata ttcttgaagt
     8941 agtcactaat tttattttat gtggaatagc gcttgtttca aaatatataa cttcatcaaa
     9001 tgaaattttc catagtgagg aatctgagta gatagtaaaa aaattagagc gataattttt
     9061 ttctttaacg tatcttttat gagcaatttt aatacaagct tttattttac tttcagcaat
     9121 ttccatatca tgttttaata taaagtccat aacttctaac ctataatcga agatccctaa
     9181 aatcatttct atatgatttg taagaaatat gattggacta atataatctg attctctaat
     9241 tttttgtgct atccataatc catcacataa ttcattactt atcttgtatt ttgcatctac
     9301 aactataaaa tatattttta ctttattttt ttcagtaagt tcttttaata acaaatttgc
     9361 attataatat aatcttataa catcgttatc aaaaccttct tttattaaaa tattatttat
     9421 tttttctttt agttttattc caaaacaaac atcattatca aaaattaata tatccactaa
     9481 ataccctcct ataaaaaatt caaaagtaaa aaaactaaaa gtattttata aaaaatacgt
     9541 aaaataacac taggagataa ataaaattaa ggatagttag aattaaggta tatttatgct
     9601 tgtttatata gtatatctta tacttatagt ttacaataat aaagtaaatt aaatcaatat
     9661 attattataa aaaatttatt tatattaaat aatatgtttt atattaactt agataataaa
     9721 aattttctgg aatttttatt ctt
//
LOCUS       DAEPOZ010000138         9651 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.138, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000138 DAEPOZ010000000
VERSION     DAEPOZ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9651
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.138"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            195..1481
                     /locus_tag="KR440_002510"
     CDS             195..1481
                     /locus_tag="KR440_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBH0699548.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            1593..2066
                     /locus_tag="KR440_002511"
     CDS             1593..2066
                     /locus_tag="KR440_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1725..1727,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HBH0699549.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            2098..3408
                     /gene="grdB"
                     /locus_tag="KR440_002512"
     CDS             2098..3408
                     /gene="grdB"
                     /locus_tag="KR440_002512"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:3145..3147,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBH0699550.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            3665..5197
                     /locus_tag="KR440_002513"
     CDS             3665..5197
                     /locus_tag="KR440_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HBH0699551.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNSDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            5208..6353
                     /locus_tag="KR440_002514"
     CDS             5208..6353
                     /locus_tag="KR440_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HBH0699552.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            6663..7742
                     /locus_tag="KR440_002515"
     CDS             6663..7742
                     /locus_tag="KR440_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBH0699553.1"
                     /translation="MSRVKNVVGLLGTKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATKLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            7855..8454
                     /locus_tag="KR440_002516"
     CDS             7855..8454
                     /locus_tag="KR440_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699554.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLPPKGQE
                     SSQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIAALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(8641..9513)
                     /locus_tag="KR440_002517"
     CDS             complement(8641..9513)
                     /locus_tag="KR440_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0699555.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
BASE COUNT         3703 a         1111 c         1841 g         2996 t
ORIGIN      
        1 tccaactttg ttaaaaaaat aattttaaat aaaataattt ttttacaata aatatttttt
       61 agtaataaaa taaagattta aggcataaaa acacaataat atatctttaa aaaaataaga
      121 taaattgtaa aatgatataa ttttgagaaa aactcaaatg catatacaca aattaaataa
      181 caggaggtgc aagtatgcgt cttgaattag ggaaaatctt tataaaagat attcaatttg
      241 gtgatgtaac agaagtaaaa gatggagtat tattcatcaa taagcaagag atgctacaag
      301 aaattggtgg agatgagcat attaaatcta tagatataga attagctcgt ccaggtgaaa
      361 gcgtaagaat aacacctgta aaagatgtta ttgagccaag agtaaaagtt gaaggtaatg
      421 ggggaatctt ccctggtatc atgtctaaag ttgatactgt aggagaagga aaaactcatg
      481 ctttaaaagg tgtagctgtt gtaactactg gaaaaatagt aggtttccaa gaaggtataa
      541 tagacatgac tggagaagga gctaagtata ctccattttc taaattaaat aatgtagtag
      601 taatagctga acctattgat ggtttaaaac aatacgctca tgaaaaagct gttagaatga
      661 taggattcaa agcagcaatg tacttaggag aagtagcaag aaatcttact ccagatgaag
      721 tttcagtata tgaaactaaa cctcttcttg aatcaataaa agaatatcca gagcttccaa
      781 aagtaggata tgtttacatg cttcagactc aaggtttatt acatgatact tatgtatatg
      841 gagtagatgc aaaacaaata gtaccaactt tattatatcc aacagaatta atggacggag
      901 caattgtaag tggtaactgt gtttctgcat gtgacaaaaa cccaagttat gttcatataa
      961 acaatggggt tgttgaagac ttatatgcac gacatggtaa agatataaac tttgtaggtg
     1021 taattataac taatgaaaat gtttacctag cagataaaga aagatcttca aactggactt
     1081 ctaaattatg taaatattta ggattagatg cagttatagt ttcacaagaa ggttttggaa
     1141 atcctgatac tgaccttata atgaactgta agaagataga aatgcaaggt gttaaaacag
     1201 ttatagttac agatgaatat gctggacgtg atggaggttc tcaatcactt gcagatgcag
     1261 atgtaagagc tgatgctgtt gtaacaggtg gaaatgccaa ccaagttgta gttcttccta
     1321 agttagataa agttattgga catttagaag tagtagatgt aatagctggt ggttctgatg
     1381 gaagtttaag agctgatgga actattgaag ttgaaatcca agctataaca ggtgcaacta
     1441 atgaaacagg atttggtcat ttaactgcta aaggatatta agaagtattt tattaaacga
     1501 ttgagtaatg ttaataaaac atattataga taattataat aagtgttata gatatataaa
     1561 aatatattag aaatataata aggaggatta ttatgagttt acttagtaat aaaaaggttc
     1621 ttataatagg tgaccgtgat ggtataccag gacctgcgat agaagaatgt gtaaaaacag
     1681 tagaaggagc agaggttgtt ttctcatcta cagaatgctt tgtctgaaca gctgctgggg
     1741 ctatggactt agaaaatcaa aacagagtta aagatgctgc tgataaattc ggagctgaaa
     1801 atgttgtgat tttactaggt gctgctgaag ccgaagctgc aggtcttgca gccgaaacag
     1861 taactgctgg agatccaact ttcgctggac cacttgctgg agttgcctta ggattaagtg
     1921 tttaccacgt tgttgaggaa ccaataaaat cattatttga tgaaagtgta tatgaagacc
     1981 aaataagtat gatggaaatg gttttagaag ttgaagaaat agaagaagaa atgtctggta
     2041 taagagaaga attttgtaaa ttttaatgcc taaaagcaaa aggggtgaaa ctaagagatg
     2101 ggaaaactaa aagcagtaca ttatattaat cagttctttg ctggtatagg tggagaagaa
     2161 aaagctgata ctaagccaca tgtggcagaa actttacctc caataagttt acaacttgat
     2221 aaattattag gtgaagatat tgaaatagtt ggaacagtag tttgtggaga tagctatttc
     2281 aatgaaaata ttgatagtgc aagtgaagaa gttttatcaa tggtaaaagg ttttgaacca
     2341 caacttttca tagctggtcc tgcatttaat gctggtagat atggggttgc tgctggaaca
     2401 ataactaaag ttgtaaaaga tgctttgaat ataccagctt taacaggtat gtatgttgaa
     2461 aatccaggag cagatatgtt caaaaaggat gtttatgtag tagaaacttc tgattctgca
     2521 gcaggtatga gaaaggcact tcctaagata gctaaacttg cagtaaaact tgctaatgga
     2581 gaagaaattg gaactcctaa agatgaagga tacatagcta gaggaataag agttaactac
     2641 ttccatgaag acagaggttc taagagagct gttgacatgt tagttaagaa aataaaaggt
     2701 gaaccatttg aaactgaata cccaatgcca aactttgata gagttgaccc aagtaaagct
     2761 gtaaaagatt tatcaaaatg taaaatagct ttagttactt ctggtggtat agttccaaaa
     2821 ggaaatccag atagaataga gtcttcttca gcttctaaat atggaactta ttctatagca
     2881 ggagttatgg atttaacaga agagacttat gaaactgcac atggtggata tgacccagtg
     2941 tatgcaaatt tagatgcaga tagagtatta cctgttgatg ttcttagaga tttagaaaaa
     3001 gaaggtgtaa ttggaaaatt acatgagact ttctatacaa ctgtaggtaa tggtacatct
     3061 gttgcaaact ctaaaaaata tgcttctgaa attggagcag cattagtggc tgatggtgta
     3121 gatgcagtaa tattaacttc tacatgaggt acttgtactc gttgcggtgc aacgatggta
     3181 aaagaaatag aaaaaactgg acttccagta gttcatatgt gtacagtagt acctatttca
     3241 ttaacagtag gggctaatag aatagttcca actatagcaa tacctcatcc acttggaaat
     3301 ccagcgcttg accctacaga agaaaaagcc ctaagaagag gccttgtaga aaaagcatta
     3361 aatgctttaa ctacagaagt agatggtcaa acagtatttg aaaaataaaa ataacacaca
     3421 catatattat atagataaaa atataaacac acactaaaaa taaaaaataa ctatacacaa
     3481 aaaaaataat atagatttca tgaatggata aattggagtt attcattcat gaaatcttaa
     3541 aaaatgccaa aatacataaa caataataga atcaaatata caataaaata caatgattag
     3601 agcttttaat aagtttaata agattaaaaa ataagtgttc ataaaattta tggaggtgca
     3661 caaaatgact tatccagttt taaaaggggc aggctatgta cttattcata ctccagatat
     3721 gatagtgcaa aatggaagta cttgtacagt tgaaagagca acaaattcag attcagaatt
     3781 tttaaaagaa gtaagtaatc atataagaag ttatgaagat gtagttaact acatgccaaa
     3841 tcaagtttat ataggaaata gaagaccaga agaattaaga gatttaccaa tgccatggtg
     3901 tgaacaaaag attgagggaa ctagaaatgg taagtttgga gaaataatgc ctcaagatga
     3961 gttcatagct ttaatgcaaa taagtgatgc atttgattta gtaaaattat cacaagaatt
     4021 tattgatgaa gtaaagccaa aaatcgaaaa taactaccct gaaattgcac catttgtagg
     4081 taaattaaag ggtgatgata tagaagaagg taaagaatta gtagcaacac acatagctga
     4141 aggattatac catgatggaa aatttgttgg ttatgtaaaa agagcacatg atgtagatgt
     4201 taacttaaat gcccatacaa tgtttgaaaa tctagtagta aaagcatctg gagttttatc
     4261 tgctattcaa atgctaagac atagcaaaat agaccctgct gaaatagatt atgttattga
     4321 gtgttcagaa gaagcttgtg gagatataaa ccaaagaggt ggaggaaact ttgctaaatc
     4381 aatagctgag atagcaggac ttcagaatgc aactggttct gacacaagag gattttgtgc
     4441 agcaccaact catgcattaa ttcaagcagc agctttagta aaagctggca tacataaaaa
     4501 tgtaatggta gtagcaggtg gagcaagtgc taaacttgga atgaatgcta aagaccatgt
     4561 taaaaaaggt cttcctgtac ttgaagatgt tgttggagga tttgcagtac ttgtatctga
     4621 aaatgatggt gtaaatccag ttataagaac tgatttaaca ggtaagcata ctgttggaac
     4681 tggttcttca ccacaagcag ttatgacagc acttataact tcaggtcttg atagagctaa
     4741 tttaaaaata acagatgttg atgtgtattc agttgaaatg caaaacccag atataactaa
     4801 gccagcagga gcaggagatg ttccagaagc aaactacaaa atgatagggg cacttgctgt
     4861 taaacgtgga gacttagaaa agaaagaatt gaaagatttt gtttctaaca aaggattacc
     4921 aggatgggca ccaactcaag gacatatacc atcaggagct ccttatatag gatttttaat
     4981 agatgattta actacaggaa atagaaatag agctatgata gttggaaaag gtagtttatt
     5041 cttaggaaga atgactaact tatttgatgg agtatctttt atagctgaaa gaaatacagg
     5101 tgttactgaa gagacttcag gaatatctaa ggatgaaatt aagaagataa tagctgaatc
     5161 tatgaaaaaa ctagcattag atatgttaga agaatagggg tgaggatatg tcaaaaaaag
     5221 taatcgcaga tgtattctta gaagtagcta atgcaataga aagcggcgaa tttggcaaaa
     5281 aagtaaaaat tggtgttact acacttggaa gtgaacatgg tgtagaaaac atggttaatg
     5341 gtgcacaatt agcaaaatct aatttatttg atattgtgct tattggacca aaagtggaaa
     5401 ctgaccttga agtagttgaa gttaatgatg aaaaagaaat gcatgcaaaa atggaagaat
     5461 tattagattc aggatatatt gatgcttgtg ttactatgca ttataatttt ccaataggtg
     5521 tatctacagt aggtagagta ataactccag caaaaggaaa agaaatgata cttgcaacaa
     5581 caacaggaac aagtgctact aatagaatag aggcaatggt aagaaatgcc atttatggta
     5641 ttgctacagc taaatcaatg ggtaacaaat gcccaaaagt tggaatactt aatgttgatg
     5701 gagcaagaca agttgagaag tgcttaaaag aattaaaaga taatggatat gacatggagt
     5761 ttgcggattc aattagagca gatggtggat gtgtaatgag aggtaatgat ttacttgtag
     5821 gtgcacctga tgttatggtt actgatacat tatctggaaa tatatttatg aaagtatttt
     5881 cttcatatac tacaggtgga gattatgaag ctcaaggctt tggatacgga cctggagttg
     5941 gagaagacta tgatagaaag gtacttatag tatcaagagc atcaggttct ccggtagtag
     6001 caaatgcttt aaaatacgct tatgatgttg taaagggaga tataagtaat gttgcaagaa
     6061 atgaatttgc aaaggttaaa aaagcaaaat ttgatgacat tatatcctct cttactaaaa
     6121 aagaagttaa agctgaaaaa gtagaagtta aaatgccaga taaagaaatt gttactcgtc
     6181 aaatagctgg tgttgatata atggatttag aagatgcagt atctgagtta tggaaaaacg
     6241 gaatctatgc tgaaagtggt atgggttgta caggtccaat agttttagta aatgaagcta
     6301 aaggagactt agcagttgaa actttagtta aagctggata tactgcaaaa taagtacatt
     6361 ttttatatgt aaataagaac atatagttat aattttattg attataatta tatgttcttt
     6421 ttattttata tcttgtcaat atttatatat taacacaatc catttatttg attctctagc
     6481 taatttatta tatttattca attaaaatta agctttctaa ctaaataaaa actaatgaag
     6541 tattaaaatg aaattaataa aatatgaaaa aattttagga ataaagtatc tgttagataa
     6601 aaataagatt aattagtgat ataatataat taagataaaa tttattattt ggaggtaaaa
     6661 tcatgagcag agtaaaaaat gtagtaggat tgttgggaac taaaggtgtt gatgcactat
     6721 atctaacaaa gaaaactaat gtaaactata taagtggttt tcctgatgaa gaagcatatg
     6781 cagtgatttg caaagatgga aactttttag ttactgatag tagatatatg gagcttgcag
     6841 aaaaggtttg caaagatttt gaaattataa attggcataa ttttgataga agtgtggcaa
     6901 aggcagttaa aagtgtatgt gataaagtgg gtataaagaa acttgggttt gaaagaacta
     6961 atatagtgtt tgataaatat gaagaactta aaaatctgat tgagaaggat aatggagaat
     7021 taatacctac agaaaatatt gtagagactt tgagatatgt aaaagacaaa gatgaaatca
     7081 aaaatacaag aaaagcttgt gaaatagcgg ataaggcatt agaagaattg attccacata
     7141 taaaagctgg agttagcgaa attgaactgg ctacaaagtt agagtacttt atgaaaatga
     7201 acggagcaca aaatataggg tttgaaacta tactgatatc aggagcaaag acttcattgc
     7261 ttcatggtaa gcctagtgat aagattatag aaaaaggtga ttttgtactt atagactatg
     7321 gtgctatgta taatggctat atatctgata caactagaac ttttatagtt ggaggagctt
     7381 ctgaaaagca attagaaata tataatttag ttaaggaagc tcaaaatgta ggggttgaga
     7441 acatgaaagc tggtgtacat gcgacaattc cagatgctga gataagaaaa gttgtcaaaa
     7501 aatatgaaga ctattactat caaggtatag gacatggagt cggtagggat gttcatgaag
     7561 aacctttcat tggtaattat ggtgataaaa tcatagaaga gggatgtata ataacaatgg
     7621 aaccaggtat ttatttccct ggatggggtg gagttagaat agaagatact gttttgatta
     7681 ctaaaaatgg tccagaaaga cttactaaat tccctaaaga tttgatgatt ttagacaaat
     7741 aatttaattt atatatattt ttttacataa aatataacat aatctcacta ttaagctcta
     7801 tttacatatt atagtaaaga gggcttaatt ttattgtaag aggtgatagt atatatgtat
     7861 aaaaaacata aatttataaa aaatataaaa aaagcctggt ataaaatcaa attaattgat
     7921 agatgcctta taattgtaat ggcaatatta atgtttcaat ctatatataa tttatttgtt
     7981 aatgaagtaa attcacaaga tactacaact attgatgtag tagtaagaac cacttcagca
     8041 gctatatttg ggtatttttt aagtgctaat tttataaaga gacctttaag gaagtataca
     8101 aatactgatg tatcttcaaa tctttttata gataataatt tacccccaaa aggtcaagag
     8161 tctagtcaaa ataatataat gaatgtcaaa aataccattg gatttacttc agaagctgat
     8221 aattatgaaa agaaaatacc tattaataat aatgaaggtt ttgaagaagg agaaactagt
     8281 gaattgcaaa ttattatagc tactgtaatt tgtatagcag cattaacagt actttttgtt
     8341 gtaagaaatt ttacaactac aacatcagct tctttaggta ctatttcaca gatgagagat
     8401 tttgtctcag gatgcgtggg ttttttgcta ggttgtccaa gtaaacctga ttaaaaaagt
     8461 attatcaata aaaggtattt taaaaagtat cagtataata atatttagat ttaaatgtat
     8521 acaattataa ttctataaca ttctatattg tatttttata ctgatactct aaactatact
     8581 atttattaaa atattttaac attgtattta aacaaaatat tatatctgta taatataaat
     8641 ttagtttata tgaatagatg ccatatactc aataaaaagt ttcatactat gagatatgta
     8701 ttgatttctt ctccaaagaa ctccaatatt tgcagtaatt gcacgttcta atggtattga
     8761 acagagattt tctgtatttt gaaatatttc tttatacaat attgttgaag ccaagtcatt
     8821 ttctagtaaa taacgtatgg tagaaagttg atttgagtgt aaaattatat ttggcttagc
     8881 tttttcaagt ctttttgtaa taatcttata gtgataagaa ccttcagata agattacaaa
     8941 aggtacattt tttaacatat aatttgatat aatgggatgt tttgccaatt catttttggg
     9001 atttgtaact aagtacaatt ctgttttata gattgtcttt gaatctatat catcataaat
     9061 atcactgtca attataccaa tacaaaaatc aagtgtagat tcttcaatca ttttagctcc
     9121 aacaaatgtt ggaacttcaa aaattttaag ctctatatct ggataaatta cattaaatct
     9181 aggaagtatt tttgaaagca aaaacgtacc taaaactgta ggaataccta ttttgagaga
     9241 agcttttctc ttttttccta aatcaagtgc gtcacattga aaatcctcaa attgttttat
     9301 aaaatgtttt gacttgtcta aaaaaatttt tccttcaggc gttaattcta ttttattacc
     9361 tattctgtaa aaaagttcaa agccaagttc tttttctaat tctttaattg ctaatgttat
     9421 ggatggttga gaaatatgaa tactttccgc agccttagtc atgtttttat atttacagac
     9481 agtttgaaaa tagtataaat agtttatatt catttggtat tagttccttt catttacata
     9541 ttagtgacaa gtatataatt aacaatagca taagttttgt atattgttgt aaaaaaatag
     9601 aatagtttta aactatcata gcataagttt ttgatatttt caatctatta a
//
LOCUS       DAEPOZ010000139         9545 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.139, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000139 DAEPOZ010000000
VERSION     DAEPOZ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9545
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.139"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..2232
                     /locus_tag="KR440_002518"
     CDS             <1..2232
                     /locus_tag="KR440_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH0699556.1"
                     /translation="KLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSKN
                     YLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGII
                     DLEGKAEFLSGKKFDLKNTASYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSIL
                     IGVSNKESMDEILNIDSFNGEGTIEIINSQGKTLFIGKNNELIKDLSYKYKDSADEPW
                     AQKMLNDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDVVLGELNKL
                     NSFTIIMTFITTCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKPL
                     ISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHDA
                     DLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLITI
                     QDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKID
                     IKSGKISGAESLVRWKNSEKGIIYPSNFIPLFEKSGFICKLDLYILEETCKLISKWIK
                     EGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAIDI
                     IEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSII
                     ELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIKN
                     IHY"
     gene            2488..4518
                     /locus_tag="KR440_002519"
     CDS             2488..4518
                     /locus_tag="KR440_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0699557.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDLIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENEIILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKEWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDEDMKWEDAVKISSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            4515..4967
                     /locus_tag="KR440_002520"
     CDS             4515..4967
                     /locus_tag="KR440_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0699558.1"
                     /translation="MNKLVNVKCSFKIHANSKEEAIVSLVDVVSKEGYLIDKNQFLED
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDIPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            4969..6024
                     /locus_tag="KR440_002521"
     CDS             4969..6024
                     /locus_tag="KR440_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBH0699559.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLIGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     IGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            6091..6399
                     /locus_tag="KR440_002522"
     CDS             6091..6399
                     /locus_tag="KR440_002522"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBH0699560.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            6503..7768
                     /locus_tag="KR440_002523"
     CDS             6503..7768
                     /locus_tag="KR440_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HBH0699561.1"
                     /translation="MKKLPIKTVVQRLLDLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKIADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLSVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVDKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKNEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            7856..8572
                     /locus_tag="KR440_002524"
     CDS             7856..8572
                     /locus_tag="KR440_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBH0699562.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHEVEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
     gene            complement(8767..9060)
                     /locus_tag="KR440_002525"
     CDS             complement(8767..9060)
                     /locus_tag="KR440_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HBH0699563.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(9066..9281)
                     /locus_tag="KR440_002526"
     CDS             complement(9066..9281)
                     /locus_tag="KR440_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699564.1"
                     /translation="MRTLSETEKISLAAVIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
BASE COUNT         3685 a          972 c         1640 g         3248 t
ORIGIN      
        1 aaattatcaa ggtataagaa aaatagttta gttctgtcta taattttatg ttcacttatt
       61 atttcaatcc ttgtaattac tgtttttaat accgtaaaac tacacaatat atctaataaa
      121 caatctaaaa actatttaaa cgatgtatct acacagattg taatgaatgt tgattctaaa
      181 atcaaattta ttttatccga cttaagaata atggcagatt ttattaagca atatgaaggt
      241 gatagtcgat atgagtattt atctaaaaga aagactagtt ataagtatta tgatgttgga
      301 ataatagatt tagaaggtaa agctgagttt ttaagtggaa aaaagtttga cttaaagaat
      361 actgcttcct ataaaaaagg aattgaaggt aaagaatact cagaagtaat agagtcaatg
      421 ggttttgttc tttatagtgt acctatattt aacgataatc atgaagtgtc tagtatttta
      481 ataggtgtat ccaataaaga aagcatggat gagattctta atatagatag ctttaatgga
      541 gaaggaacta ttgaaattat taatagtcag ggaaaaactc ttttcatagg aaaaaataat
      601 gagttaataa aagaccttag ctataagtat aaggattctg cagatgaacc ttgggcacaa
      661 aagatgctta atgattttaa aaatgataaa tcaggaaata ttacaataac ctcatcaaaa
      721 ggtgtacaat gcttattaac ttatcatcca attacaagag gtttaaatga ttggcatttt
      781 cttctcatag tacctgaaga tgttgtactt ggtgaactta ataagttaaa ttcttttaca
      841 ataataatga catttataac tacatgtatt attggaatta taacattaat tttatatatt
      901 ataagaagaa aatatgtaaa tcaaatagaa aatattgcat atttagatag tataacaaat
      961 ggtataaatt caactaagtt tagtatgcta gtcaaacctt taatttcaga atcacctgat
     1021 agtacttata tgatgatagc aatgaatata aaagatttta agctaataaa tgattgtttc
     1081 ggtagtgaaa aaggaaatat gacactaaaa catctttaca atattttgta taaaaacatt
     1141 gatgaaaatg aagaatttgt gtgccgtcat gatgcagacc ttttctattt gcttattaag
     1201 aatagaccag tgttagaagc cctggagtta ttatataaaa tagaagctga cacaaattat
     1261 tttaataaaa atagggaaaa tccatacttt ttaagactat cagtaggtat ttacacaatt
     1321 gaaaatcata atgaagattt aattacaata caagaccatg caaatacagc aagaaaaagc
     1381 tttaataaaa ctcatcaaag tgatttttct ttttatagtg atatagaaag aaaacgatta
     1441 atatctgaaa aagaaatttc aaatttaatg gaaaaagccc ttgaaaataa agaatttttt
     1501 atgtgcttac aacctaaaat tgatataaaa agtggaaaaa ttagtggagc agagtctctt
     1561 gtgcgttgga aaaattcaga aaagggcata atttatccaa gtaattttat tcctcttttc
     1621 gaaaaaagtg gatttatatg taagttagat ttatatatac tagaagaaac atgcaaattg
     1681 atttctaagt ggataaaaga aggtaaagaa gtgttgagaa tttctgttaa tgtatcacgt
     1741 caacatttga atgataaatt gtttttagaa aaatataagc atatatgcaa taaatacaat
     1801 gtaccaacat gtctgataga tttagaactt accgaatcta ttttcttaga aaatccagaa
     1861 gctatagata taatagagga tattcattct aatggattta aatgttctat agacgatttt
     1921 ggatttgggt actcatcatt agggatatta aaggatttta aagttgatat aattaagtta
     1981 gaccgttcat tctttgttag caaaaataat atagaccgtg gtaaggtagt tattaagtct
     2041 attattgaac ttagcaaaag gctaggtatg aaagtaactg ctgaaggaat tgaagaatta
     2101 gaacaagttg agttcctaaa aaatgtagga tgtgattata ttcaaggtta tgttttttct
     2161 aaaccattat taatttctga ttttgaaaat tttgcatatg aaaataataa aattaaaaat
     2221 atccattatt aaatgtacaa gataaaaaat ttaggcaagt tatataatta aaaatataac
     2281 ttgtcttttt tatttatata tttaataaaa atatatttgc agattttgta aaaataattt
     2341 tcaaatgttg taagttgtgg ctaaagcttt attgtattat tctgaatata gaaagatagt
     2401 tactgtctta gataaaaact gaaacataca taataatata aaatttgcta tattaataac
     2461 tctaattatt ttggaaggag ggaaaatatg ataagtggga gaatgttact catcatgaat
     2521 tttctaaaag gaaaaaataa gacaagttac aaggatatta gcaaacaact tagtatagaa
     2581 gaaagaaaag ttagatatga tataaataat ttaaatattg ttttgcgttc attaaacaag
     2641 tcagtaatac aaaagatgaa caaaggtgtg ttaattattc ctgatgactt ccaagtagta
     2701 tgtataaata ataacgaata tgtattttcc ttaactgagc gagtatctat tatgaaaatt
     2761 atatcaatgt ttaaaataga taatctaaac ttagaaaaat tgagtaaaga atttcaggtt
     2821 tctagaagtt caattaaaaa tgaccttaat gttttgtcaa gtgaattaga aagaaaccaa
     2881 ataactatta cttatgataa atcttttaaa ataagtggag aagaagacct tatatataag
     2941 caaagattga acatattaaa atcgtattct tatctttttg gtaaggaaaa atctgattta
     3001 agtgtatttg agaagtattt agtgaatgta attgaaaaaa tattcaaagg gatttatttg
     3061 attgatatat ataattggtc tatgaattta ttattgcaaa tgggatggac tttaaatgat
     3121 gaatctttta catggtatgt gtcaaacatt ttccttttta catggtatat acattcagat
     3181 gaaaaacatc ctttagaatc tgcttcatta agtgagccat gttttgaaaa taagtttgac
     3241 caagaatttg aaacaattat tcaaaaaaag ttgactaagg aacaaatttt tctgttaaag
     3301 agttttgtat tttttacaaa taaatatgca agtttaaacg aggatatgga tttgattagt
     3361 acagaaatta tagttcaaga cctaatttac aatatgtcag aaatattaga gattagcttt
     3421 aaagaagata tgattcttta taaaggatta ctcaatcata ttgccccatt gatagagaga
     3481 atcaagcaga atatacagat ttatgaggaa ttacttcatg taataccaga taaatatcaa
     3541 tatgtacttg aagctactaa ggtttctatt caaaaaaaca gagttttaag tcaaataaag
     3601 aatgaaaatg aaataatact tttggcaata catttcttag gaagtataca tagaaatgag
     3661 aggaataact acaaaaatat attgttagtt tgtggatttg gatatggtgt aattgctatg
     3721 ttaaaggata gtttagtaaa tgattaccag ataaatatag tagagagtat tccatcatat
     3781 aagttacaag attataaaga atgggaaagt attgacttca ttataacaac atcaaagata
     3841 gtaatagact tgcctaaaac aattattgag ataaatccat ttttacaaga agaggattat
     3901 ttgaagtttg aagaaaaagg gataaaaaga aagaacgtct taactaatta tatgtcaata
     3961 aaaaaaaggt tggacttttt agaagaagat gctcaaaaaa gagtattaag tattatatgt
     4021 caagaacttg gatacagtga tgagagaata ttatttagac aacttaattt aagtgattta
     4081 atagggattg ataccataag gataattgat gaagatatga aatgggaaga tgctgtaaaa
     4141 attagttcaa acatacttgc taattgtgat tttgtatcag cacaatatgc tgagaatatt
     4201 atagatattt taaaaaatgt gggtttttat gctgtaaaag ataacgaatt tgctttacta
     4261 catggcaatg atagttcatt agtcaaagta agttcgataa gtttattaat atgtaaagaa
     4321 gcagttagtt ttggagataa aaaggttaaa atcatatttt gtctagcaag cagagataaa
     4381 aaagagcaaa ttcctgcaat aattaatcta actaaaatgg tttataagac taattttatt
     4441 gctatgttag aaagtgcaaa gacaaaagaa gaagcagaag ttttaattca taaatatgag
     4501 aaggaggtca ctgtatgaat aaattagtta atgtaaaatg ttcatttaaa atacatgcta
     4561 attcaaaaga agaagctatt gtatcgttag ttgatgtagt atcaaaagaa ggatatctaa
     4621 tagataaaaa tcaatttttg gaagatgttt taaagagaga agaaacattg tcaacttata
     4681 ttggacatgg tataggtctt cctcatagtc aaagtgtagg tgttaaaaac tcttgtatta
     4741 caataggaaa gcttgatatt cctattgaat ggacagaaga aggagaaaaa gtggatttaa
     4801 tatttttaat atcagtaaca aaagacaatg aaaataatct tcatttaaag attttatcaa
     4861 aattagcacg tttattgatg catgaaagtt ttagaaatca aattagatgt tcagatgaac
     4921 aaactgttta taatcttata aaagaaaaaa tagaggagga agattaaaat gagaatttta
     4981 aagtttttgc aaaaacatct tatgacagca acatcttata tgattccatt tgtggttgca
     5041 ggaggaatat tatttgcttt atcagtaact ctaagtggac aagctgctgt tcctgaaaca
     5101 ggctggttgg ctaaactgaa tcaaattgga gctgctggct tggctttatt cattcctata
     5161 ttaggtggat atatagcatt tagtatggca gataaaccag gattagctcc aggtatgata
     5221 ggtgcatatt tagcaaaaga agtaaatgca ggttttatag gtggtatcat agcaggattt
     5281 atagcaggat ttgttgtttt acaattaaag aaaattaaat tagctcctac aatgagaact
     5341 ttaggttcta tatttattta tcctttgatt ggtacattga ttacaggtgg tataattgta
     5401 tttttaattg gagaacctat agcttctttt atgacatgga tgacaaactg gttaaatgga
     5461 atgtctggtg tttctaaaat tcctttaggt ggaatcttag gaggaatgat agcctcagat
     5521 atgggtggtc ctataaataa ggttgctgct acttttgccc aaactcaagt tgacacactt
     5581 ccatatttaa tgggtggagt tggcgttgca atttgtatac ctccaattgg tttaggatta
     5641 gctacattat tgtttccaaa aaaatttagc aaggaagaaa gagattcagg taaagcttcg
     5701 ctattaatgg gatgtgttgg aattacagaa ggagctattc catttgcaac atcagatcca
     5761 gttagagtaa taccttgtat tatggctggg tcgattatag gaaatattat ggctttttta
     5821 ttaggatgtt taaatcatgc accctgggga ggtctgatag tattacctgt agttgacaat
     5881 aggttggggt atattgcatc agtattaact ggagcagtag ttgttgcagt attaatgaaa
     5941 ttagtaaaaa aagacgtgaa agaagatgaa gaaattgaag aggatttaga tgaatcaatt
     6001 gaattaatat ttgaagaatt ataaatataa atggaaggaa tttgtataaa aaagtaaata
     6061 taaaactata aacttaaagg agataagaat atgaaaattg tagcagtaac atcttgtcct
     6121 agtggagtag cacatactta catgtcggct gaatcacttg aattatcggc caaaaaattt
     6181 ggtatagaaa taaaggttga aactcaagga agctctggaa tagataatga attgtcactt
     6241 aaagatattg atgaagcaac ttgtgtaata ttgactaatg atgttgagat tagaaatatg
     6301 gaacgtttta agggaaagaa ggtagtaaga atgagtgtat ctgagataat taagaaatct
     6361 gatgcattaa ttaagaaaat aaaagatact tttcaataaa aattgattta ttttacatat
     6421 aactcaaaga tgtttttaaa gaaattttta ttaaaagcat ctttgaaaat atcttaaaaa
     6481 tagaataaaa ggagatatag tcatgaaaaa attaccaata aagactgtcg tacaaagatt
     6541 actagactta caagaagagg ggaaaagtgc aactctttta ggaattggtc ctatgtcacc
     6601 aaacttgtta caagcaagtt ttgaactagc aaaagatgat gattttcctt taatgtttat
     6661 cgcaagtaga aatcaagtag atgcagatga actaggtggc ggttatgtaa atggttggaa
     6721 tcaggaaact tttacaaaag atattaagaa gattgccgac aaagttggat ttgatggttt
     6781 atattattta tgtagagacc atggtgggcc atggcaaaga gataaagaga gaaatgacca
     6841 tttgtctgtt gatgaagcaa tggagttagg aaaaaaatct tatttagcgg acattgaagc
     6901 aggttttgac ctattgatga ttgaccctac aaaagaccca tttgaaattg gaaaagtaat
     6961 tccactagat gtagtactag aaagaactgt agaattaatt gagtattgtg aaaacgagag
     7021 aaaaagactt aatctaccag acataggtta tgaggttggt acagaggaaa ccaatggagg
     7081 tcttacttca actgagacgt atgaaacatt tataactaga ctaaaggttg aacttgataa
     7141 taggggtttg ccaatgccaa catttatagt tggtcaaact ggtactttaa ctagaaagac
     7201 agaacaagta ggaactttta attttagaaa tgcttatgat ttagctcaaa tggctaagaa
     7261 atatggagtt ggtttaaaag agcataatgg cgattactta gatgatgtaa ctttattaga
     7321 acatatacca tctcaaatta ttgctacaaa tgtagctcct caatatggta ctgaagaaac
     7381 tagggcatat ttaaaattag cagaagttga gtgtaaatta gaaaaagagg gcctagttga
     7441 taaaacttct aatatcagac atgttcttct tgttaatgca attgaatgtg gacgttggag
     7501 aaaatgggta gtaggagaac aaaaaaatct aactactgaa gagatattta agaatgaagt
     7561 tttatctaat gaaatactag atattgcagg acattataca tttaataatg atgatgttaa
     7621 aaaagaaata gaagtgcttt atgataattt aagtaagaat aatattgatg gtcaaagatt
     7681 tgttgtagac catattaaac gtcctttgcg tgattatgca gaatgctata acttaaaagg
     7741 tgttactacg aggattttaa ataaataata ggacttatag tggtgagtat tgttatacta
     7801 ggtatttata gttataatta tacttaccac tattaaattt aggaggacta atattttgat
     7861 taatttaatt tttgatgtag atgatacttt atataaccaa ttaacgcctt tttacactgc
     7921 atataataaa gtgttttctt caataaagga tatttctatt gaagatttat atatgagtag
     7981 tagaaagtat agtgatgagg tatttcatat gactgaaaat ggagaaatgc caatcaaaga
     8041 aatgcatatt tataggataa tgaaagcttt tgaggagttg ggaaattcta ttacagaaaa
     8101 agatgcacaa agttttcaag atgagtatat atatcagcaa tcacaaatta cactaatacc
     8161 agaagttgag aggattttaa atttttctaa agaaagaaat attaatctag gaattataac
     8221 taatggccca tcaaaccatc aaagaatgaa gttaaagcag ttaaatattg aaaattgggt
     8281 tgataaaagt aatattttta tttctagtga ggttggattt tctaagccag atactaatat
     8341 ttttagagta gctgagaatg ttatgaattt agatagagaa aatacatatt atgtgggtga
     8401 ttcttataga aatgatgtat taggtgctaa aaaagctggt tggaagagta tatggttgaa
     8461 ccatagagga catgaggtag aagagttgtt ttataaacct gattttgtaa ttttggaaca
     8521 taaggatttg atatctttat ttataaaaat atgtagttat aaaaatatgt agtaaaaaaa
     8581 tgttaataat agaattgtat aaaataatta ataataacta tataaaatta tatgtttaga
     8641 ttgtgagcaa tctattttat gttaaaaata gattatttag aagaagggga aatcttaaaa
     8701 aaatttgaat atcccctttt tattatttaa ttaaaatgaa atagttagaa atatatttta
     8761 tttagtttag tcaataacat tttgggattc tttataacta catgttaact gttgaatagg
     8821 cttatcatat ggtttaaccc atcctttatt aatacttaat tctgtaagtg ctgtgtgacc
     8881 ttctaccatt tgagttagtg aagctgaata tatagctctt aattcaggtg tactacttgt
     8941 taatgcagaa ttaagataca tattggcaga tgctttagca gatgaaagca tacttaacat
     9001 tattacttta tcgtctatat aagtattgtt cttaattaga ttttctatca tatttctcat
     9061 gttatctata cctcctcaga aattagtatt tcattttcaa ctataaattg ttgtatagct
     9121 ttaattcttc cttctgttgc taatatacta gattctgatt gtctttttag gtcttcatct
     9181 gatattaaca tatttattgc tctttgcata agtaatccgt cagactccat ttttataact
     9241 gctgctaaag atattttttc agtttctgaa agtgttctca tttaatcatc tcctatattg
     9301 taatttatgc tttaatagta tgtctttctt ttcaaggaaa taaccattac acttttttac
     9361 agtattttat attctcttta tatcaaagtt tagtcaactt aatatattaa tataattttg
     9421 taggatattt atcattaaat atctataaaa taaattattt gaatattcat aaatcaaaat
     9481 ttatttgtat gtacttcttc aagagtcaat atgaattttt tcaaaaaatt aattttgatg
     9541 ataat
//
LOCUS       DAEPOZ010000140         9499 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.140, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000140 DAEPOZ010000000
VERSION     DAEPOZ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9499
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.140"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            101..877
                     /locus_tag="KR440_002527"
     CDS             101..877
                     /locus_tag="KR440_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBH0699565.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKNSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGVGDLKELYRNINYHKLPSSSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMGKTNQFTLENVKQRKNK"
     gene            complement(969..1721)
                     /gene="xth"
                     /locus_tag="KR440_002528"
     CDS             complement(969..1721)
                     /gene="xth"
                     /locus_tag="KR440_002528"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBH0699566.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(1882..2832)
                     /gene="galT"
                     /locus_tag="KR440_002529"
     CDS             complement(1882..2832)
                     /gene="galT"
                     /locus_tag="KR440_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBH0699567.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIESEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
     gene            3217..4413
                     /locus_tag="KR440_002530"
     CDS             3217..4413
                     /locus_tag="KR440_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH0699568.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
     gene            complement(4525..5871)
                     /locus_tag="KR440_002531"
     CDS             complement(4525..5871)
                     /locus_tag="KR440_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBH0699569.1"
                     /translation="MTMKKRYNIAIVGGGSTWTPGLLKSLCKMSDRFPLNKVTMLDVV
                     EERQRLIGEFGKILFKEEYPKATLEYTTNPDEAFIDVDFVFVQMRTGGLKMRELDEKI
                     PLQFGLVGQETCGAGGFAYGLRSIGDMIEMVKTVRKYSPKAWILNYTNPAAIVAEALK
                     RVFPDDKRLLNMCDQPVNLLRSYGRLLDMDSRTFEPVYFGLNHFGWFTHLYDKNGEDL
                     VPKIKDLVANSGFQPVDAEQRDKSWLDTYGMVKDMLEDVPDYLPNTYVQYYLYPDKKV
                     AKSNINCTRAREVMNGREKRVFEECKSVIEKGTSIGSNLVHNDAHGEFIVEVAEAIAH
                     NKHQIAIVITENNGAINNLPDDAMVEVAAILTKNGPRPLHVGNIPTFYKGLLEGQLAY
                     EKLAVDAYFEQSYEKAILSLTLNRTIISPTKARQVLDALIKVNKDYWPTLYKENEK"
     gene            complement(6004..6756)
                     /locus_tag="KR440_002532"
     CDS             complement(6004..6756)
                     /locus_tag="KR440_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH0699570.1"
                     /translation="MNLIDKLDINQKKLSTNEISLLNYFLKNSEKINQKKIKDIANET
                     FNSTAYIVRFCQKLGFSGYSEFKNFLVFSYSNRNERNKEFTKSQSDIFTDVMDTQNLI
                     NVENIDMCLNEIHNSKHIYFFGVGSSRLVCTEMSQRFSAIGVNVKYYDDSTLMYLAAS
                     NITKDDLVIAVSMSGETAQVIKACNIAKTNQVKVISLTNISVNSLSNIADIKLFVSSP
                     QYNFNSVNYTSRVPALSLMEYMFHKYIEKYNR"
     gene            complement(6915..8492)
                     /locus_tag="KR440_002533"
     CDS             complement(6915..8492)
                     /locus_tag="KR440_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH0699571.1"
                     /translation="MKQKMKEFFLQLGKSFLLPIALISAAGIFLGISSAFSNPNIVSD
                     IPFLENNFIQGILGFTKAITGVLFGNLAIFFAISISVGLAKEEKAVSAFSGFVGYLVL
                     HTSVNYMLGCRGILAEPDMMREMGQAVTLGIQTLELGVFGGIVVGIITASLHNKYYNI
                     KLPDYLGFFGGTRFVPIITTVVFSILGIIIPYIWPYVNTIIQSVGIGIASLGYLGTFV
                     FGFLERLLIPFGLHHILNAMFRFTEVGGSLVVGGQEVFGALNIFLAQLSDPETISFST
                     EATRFLAQGKIPIMVFGLPAAAYAMYKTAKPENRSKVKGILLAGALATFVTGITEPLE
                     FSFLFVSPILFVIHAFLSGISFMMMHIFGVAIGNTQGGIIDLVVFGVMQPNTKWIYSI
                     ILGLVYALVYYNVFKVLILRFNLQTPGREEITDINFDLEPLSNKVEIAQSGETTKRAL
                     SIIKYLGGKENIEDITNCITRLRVVVKDMSKVDENAFKKYTGAMGVIKVSDKDIQIIY
                     GPSVSQVRDEVSFAMSI"
     gene            complement(8905..9357)
                     /locus_tag="KR440_002534"
     CDS             complement(8905..9357)
                     /locus_tag="KR440_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0699572.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
BASE COUNT         3338 a         1490 c         1109 g         3562 t
ORIGIN      
        1 ggtatactaa aataaaagta tttttatttg aaaatttaat tttatttata atataatcta
       61 tagtaaaaga aataataaaa aaatttagga gataaacttt atggaataca taattttcga
      121 tttggaattt aaccaaggat ttgataaaaa actcaataaa actgtatcca atgaaaagtg
      181 tccttttgaa attatacaaa taggagcaat taaattggat tctaaattca atataataga
      241 tacgtttaat agctatgtaa aacctacaat ttataaagac atacatccct ttattagtcg
      301 tatgactaat attaaaaatt ctgattttaa tgattcgcct actttccctg aggtctacaa
      361 caattttata aaatttatat catcacaaga ccctatatta tgtgtttggg gagttggaga
      421 tttaaaggaa ttatacagga atatcaacta tcacaagcta ccatcaagtt ccttacctaa
      481 atcttatatt aatatacaac aacatgcatc taaatatttc aataatccag caggaaaaag
      541 cattggccta caaaatgcta tttctatatt ggaacttgat gaaaaaatgt cttatcacaa
      601 tgctttaaat gatgcatatt atactgctaa agtttttatt aagatataca atccaagtat
      661 agtcccagat atatatcttt atacgagtat aaaacctaaa actataagat attctaataa
      721 aaaaagagtt gattatgata agttatttga tgaatttagg aaaattttaa atcgtgaatt
      781 aactaaagac gagaaaaaaa tcattaactt agcttataat atggggaaaa caaatcaatt
      841 tacattagaa aatgttaagc aaagaaaaaa caaatagagt ttatttagat aaaaaatagg
      901 catgtagatt aaaaaactag ttaactttaa atttatatct acagcccaat ttttatcgta
      961 attaattttt attttatctc taattcaact ggacaatggt ctgaacctaa tatttcagtg
     1021 tgtatatccg cacttactaa tctatcttct aattttttgg atgcacaaaa ataatctatt
     1081 ctccaccctg cattattttt tcttgcatta aatctatatg accaccaact ataaacgcct
     1141 tctttatcag gattaaaata tctatatgtg tctataaacc cactgtctaa taatttagaa
     1201 aacttctctc tttcttcttt agtaaaacct gcatttttca tattattttt aggatttttt
     1261 aaatctattt ctttatgtgc aacgttcata tctccacaca caattacagg cttatgatta
     1321 tctagtttgg ttaaataatc tataaagtcg tcttcccatc tagttctata ttctagtctc
     1381 tttaattcag attgagaatt tggcgtatat actgtaacaa agtaaaaatc ttcaaactct
     1441 agagttatca ctctaccctc tttgtcatgt tcttctatat ttattccata cattactttt
     1501 aaaggctctt tttttgtaaa tatagcagta cctgaataac cctttctctc agcataattc
     1561 caatattgaa aatatcctgg taaatctaac tctatttgcc cttcttgcaa tttagtttct
     1621 tgtaaacaaa atatatctgc atctacttct ttgaaaaagt ctaaaaatcc tttcccaaca
     1681 caagctctta ttccattaac attccatgat ataaatttca tacttatact cctcatctaa
     1741 acattgacac actccccata gctaaagatg ggggcttttc aacaatttta ttgtaaattc
     1801 ttaatttact tttattttaa caatactata ctataatttc atctataatt actatttact
     1861 taatacctaa aaatatatgt tttaatcaat ttttaacttt tttgttaaat cttcagggct
     1921 cattgaagaa acatacatat ttgttccaag ttcaaatcct ccaaaattat attttcttgg
     1981 gattatgtgc atatgaacat taaaatacga ttttgtttca atctttgttg gatgagtgtg
     2041 aatacataag ttaaatggca tatatccatt gatattgtat aacttcttaa acaacttttc
     2101 aaatattgta gaaagctcct gtatattatt atcatttaac tcaccaaact ctattttatc
     2161 tttaaatatt attcttactt ctccactata cttggttgca tatggtataa gtaccaaaaa
     2221 agcttctcca ttataaacaa ctcttctatc taaacttatt tcattttcta ttaaatcatc
     2281 atacaaagag cttttattgc tgtcataata ctcctttgaa actaacagtt cattcgtaat
     2341 ttcaggtgga ataaaagaca tagacaatat ttgagcatgt ggatgcatca aagatgctcc
     2401 tgcctttctc aagaagtttt tgaagatact aacatactct actttatcat ctttcgacaa
     2461 atctctaaat ctatttctat acataaaaaa aacatcttca aattcttttt gactcatgtt
     2521 ataaaaacta cgattatgtt tatcgctctc tatcattact tcatgaacac catatacatc
     2581 tcttgccata tcatcaatta taggaaattt attgtagaca gattttacaa gccatccttc
     2641 ttcactttct atttcaaata atgcatcagt tgcatgagct tcatttcctt tgcaaaatgg
     2701 gcattcttca tcatattcat tagtttcttc atcttcattt gggatatctg ctgtatctaa
     2761 aggtcttttt aacctatctg tagcaaatat aacaacatca ttagtaattg gatctattct
     2821 taattctttc ataattaaca cctcttagtt aatagccttt aatataatta tatgatattt
     2881 atcgtatctg ttaaataaat ttataatatt ttttataatt ttgcatattt attattatat
     2941 attttgtcta tagctgaaag attataacta aaaaatcact atcagtgtat cagaatatta
     3001 tcttactata aattaattta tgaatacata atttagtggc ataaacatgg gtattttact
     3061 ataatttgtt aaaagtttat tttaaagcta tattaatttt ttttgatgat ttttacccat
     3121 aagtatgtta ttattatctt gtaaagatgt cttgtattca aacatacaat ttacataata
     3181 caatatacgt atttaaatta aatagggggt attaaaatga attattcaaa cagagttagt
     3241 gcaatgcaag cctctccaat cagaaaactt gttccatttg cacaagcagc aaaagataaa
     3301 ggaatcaaag tatatcactt aaacatagga caaccagaca taaaaactcc taaaggattt
     3361 tttgatgctg ttaaaaactt tgatagcgaa gtcttagaat atgctacatc agaaggtatt
     3421 ccagaattat tagaagcact tcaaaattac tataaaactt acgatatgaa ttttgaaaaa
     3481 gatgaattat tagtaactaa tggtggtagt gaagctttac ttttcactat gatggcagtt
     3541 tgtgacccag gagataatct attagttcca gaaccattct atacaaacta caatggattt
     3601 ggtcaatctg taaatgtaga agttaatgct gttacaacaa aggcagaaaa cggtttccac
     3661 cttccatcta aagaagaaat attgtctaaa gtagatgata aaactaaggc tataattctt
     3721 tctaacccag gaaatccaac tggtgcaatt tatacaaaag aagaacttaa tatcctagct
     3781 gaaatagcta aagaaaaaga tttatggata atagcagacg aagtttatag agaatttgtt
     3841 tatgatgggc ttgagtatac aagctgtggt aacttagagg gtgtacagga tagagttata
     3901 ataatagaca gtgtttctaa gagatacagt gcttgtggtg ctagaattgg ttctatcgct
     3961 tgcaaaaata aaggtcttat cgctcaaata cttaaattat gtcaaggaag actttgtgtt
     4021 cctacattag agcaaatagg agctgttgag ttatacaaaa ctcctgtttc ttacttcaaa
     4081 gaagttaatg aagagtacaa aaaaagaaga gatgtattat acaatgaatt aatgaaggtt
     4141 gaaggtgtta tatgtaaaaa accaactggt gctttctata tagttgctaa attaccagtt
     4201 gaaaatgcag aagatttcac aatttggatg ttaaaagaat ttaacaaaga taatgaaaca
     4261 gttatggttt gccctgctga aggattctat gctactccag gtttaggacg tgatgaaata
     4321 agattagctt acatcttaaa tgaaaaagac cttcatagag ctgctacatt attaaaagaa
     4381 ggtttagagc aatatgtagc tttacaaaaa taatttttta aacttaattt atataaattt
     4441 aatgatataa aatgtcccct tttcagatat tatttgaaaa ggggacattt cttaattaat
     4501 atattaatta agacttagac ctgactattt ttcattttct ttatatagtg ttggccagta
     4561 atctttattc actttaatta aagcatctaa aacttgtctt gccttagttg gagagattat
     4621 agttctattt aatgttaaag ataaaatagc tttttcataa gattgttcaa agtatgcatc
     4681 aactgctaac ttttcataag ctagttgacc ttctaacagt cccttataaa atgttggtat
     4741 attacctaca tgtaaaggtc tcggtccatt ctttgtaaga atcgcagcta cttctaccat
     4801 tgcatcatca ggtaagttgt taatagcccc attattttct gttataacta tggctatttg
     4861 gtgtttgtta tgagctattg cttctgcaac ttcaactata aattccccat gagcatcatt
     4921 atgcactaaa ttagaaccta tagatgttcc tttttctata acagatttac attcttcaaa
     4981 tacacgcttt tctcttccat tcataacttc tcttgctcta gtacaattta tatttgattt
     5041 agctactttt ttatcaggat atagataata ctgtacatat gtattaggta agtaatctgg
     5101 tacatcttcc aacatatctt taaccattcc ataagtgtct aaccaagatt tatctctttg
     5161 ttctgcatca acaggttgga atccagagtt tgctactaaa tcttttatct taggtaccaa
     5221 atcttcacca tttttatcat acaaatgtgt aaaccatcca aagtgattta atccaaagta
     5281 tacaggctca aatgttcttg aatccatgtc taacaatctt ccataagacc ttaacaagtt
     5341 aactggttgg tcacacatat taagtaatct tttatcatct ggaaatactc tttttaatgc
     5401 ttctgcaaca atagcagcag gatttgtata atttaatatc catgctttag gagagtattt
     5461 tcttacagtc ttaaccattt ctatcatatc acctatagac cttaacccat aagcaaatcc
     5521 accagctcca caagtttctt gaccaaccag tccaaattgt agaggaatct tttcatctaa
     5581 ttctcgcatc ttaagaccac cagttctcat ttgaacaaat acaaaatcta catctataaa
     5641 tgcttcatct gggtttgtag tatattcaag tgttgcttta ggatattctt ctttaaataa
     5701 tatttttcca aattccccta ttaatctttg acgttcttct actacatcta acatagttac
     5761 tttatttaga ggaaatctat cactcatttt acataaagac tttaaaagtc ctggagtcca
     5821 agtactgcct cctccaacta tagctatatt gtatcttttt ttcattgtca tattaatcac
     5881 ccctgttttt tattaactaa cttgtaagat gattataata tataaagttt tctacatgaa
     5941 tacttttagg taatcattaa aaatagttca attttagtat ttttttcaaa attgtaattt
     6001 ttttcatcta ttgtattttt caatgtattt atgaaacata tattccataa gagaaagtgc
     6061 tggcactcta gacgtatagt taacactatt aaaattatat tgtggagaag atacaaataa
     6121 ttttatatca gctatatttg ataatgaatt gacacttata tttgtcagag aaattacttt
     6181 aacttgattt gtttttgcta tattacatgc cttaataact tgagcagtct ctccagacat
     6241 agatacagca attacaaggt catctttagt aatatttgat gcagccaaat acatgagagt
     6301 tgaatcatca tagtatttta catttacacc tatagctgaa aaacgttgag acatttcagt
     6361 acatactaat ctagaagacc caacaccaaa aaaatatata tgcttagaat tatgtatttc
     6421 atttaaacac atatctatat tctctacatt tattaaattt tgtgtatcca taacatcagt
     6481 aaaaatatca ctttgacttt tagtaaactc cttatttctt tcatttctat ttgaatatga
     6541 aaaaactaaa aagtttttaa attcactata tccagaaaag ccgagcttct gacaaaacct
     6601 tactatgtat gcagtcgaat taaaagtttc atttgctata tctttaatct ttttttgatt
     6661 tattttttca gaatttttta gaaaatagtt taaaagagat atttcatttg tagataattt
     6721 tttttgattt atatctaatt tgtctattag gttcatttat acctccataa taatgatttg
     6781 tagttaaaca actttctcat atttattata taaatataca ctatacaaca tttatcttat
     6841 gtttattata ctacaaaaaa gacaatacaa attataaatt agtattgcct tttaatattt
     6901 acattagata gacattaaat agacattgca aaagaaactt cgtctcttac ttgagataca
     6961 gatggcccat aaattatttg gatatcttta tcagatactt ttataacgcc cattgcacct
     7021 gtatattttt taaaagcatt ttcatctact ttactcatat ctttaactac tactcttaag
     7081 cgagttatac agtttgttat atcttctata ttctccttac cacctaaata tttaattatg
     7141 gataatgctc tcttagtagt ttctccactt tgagctattt ctactttatt tgataaaggt
     7201 tctaaatcaa aatttatatc tgttatttct tctcgtcctg gagtttgtaa gttaaatcta
     7261 agtatcaaaa ccttaaatac attataataa accagtgcat acactaaacc aagtattata
     7321 gaataaatcc actttgtatt tggttgcata actccaaata ctactaaatc tattatgcca
     7381 ccttgagtat ttcctattgc tactccaaat atatgcatca tcataaatga tatacctgat
     7441 aaaaatgcat gtataacaaa taatattggt gatacaaata agaatgaaaa ttccaatggt
     7501 tccgtaattc ctgttacaaa tgttgctaaa gcaccagcta gtaatatacc tttaaccttc
     7561 gacctatttt caggctttgc agttttatac atggcatatg ctgcagcagg aagaccaaat
     7621 accattatag gtattttccc ttgagctaaa aatctagttg cttctgttga aaaactgatt
     7681 gtttcagggt cacttagttg agctaaaaat atgttcaatg ctccaaatac ttcttgtcct
     7741 cctacaacta aagaacctcc tacttcagta aatctaaaca tagcatttaa tatatgatgt
     7801 aatccaaatg gtattaaaag cctttcaaga aatccaaata caaaggttcc taaatatcct
     7861 aaactagcta taccaatacc tacactctga ataattgtat ttacatatgg ccatatatat
     7921 ggtattatta tccccaaaat agagaatact actgttgtta ttattggaac aaacctagtt
     7981 ccaccaaaga accctaaata atctggcagt tttatattat agtatttatt atgtaatgat
     8041 gctgttatta taccaactac aattccacca aatactccta actctaatgt ttgtatgcct
     8101 aaagttacag cttgacccat ttctctcatc atgtcaggtt cagcaagaat acctctacac
     8161 cctaacatat aatttacact agtgtgtaat actaaataac caacaaaacc agaaaatgca
     8221 gaaactgctt tttcttcttt agcaagacca acagatattg atatagcaaa gaatattgct
     8281 aagtttccaa aaagtactcc tgttatagct tttgtaaaac ccaatattcc ttgaataaaa
     8341 ttattttcta aaaaaggtat atcactaact atattaggat ttgaaaatgc tgaacttatt
     8401 cctaaaaata tacctgcagc agatattaat gctattggca ataaaaatga ttttcctaac
     8461 tgtaaaaaaa actctttcat tttttgtttc atttctatcc ctccattttt aaatttttaa
     8521 aacgttttct atgtcataat tatatatttt tattttgttt ttgaaacttt ataatttgtt
     8581 ttaaaatatt tttacataaa tagtaatttt ttcaaaactg taaatttttt caaacaaaga
     8641 catagtaaca ttgtgatata tagaaattta aaaagagatt agaaaataaa attattgaat
     8701 ataaaatgga aaaatataaa ttacacaaag aggtatgagt aggtactaac aaataagaaa
     8761 atgtataaga aattataaaa aagtacatca agaaaattta agatgtactt tttagattgt
     8821 atttagactt ataattatat agttaaatga ataacaaaga attaatctta gtactattaa
     8881 atataaaaat tttttatatt ttttctatct aaattcttct ttgtttaata tatattgaag
     8941 agtatttatt gcttctaaat atttttttct gtcttcttca gttaatttag aaagtttttc
     9001 tcctaaacca tcacttaatt tatccataaa atcagttgct atcttatctc cctcttcagt
     9061 aatgtcaatt agatattttc ttctatcaac taaatctctt ttccttgtta catacttctt
     9121 atcagttaaa tcatcaacca tactagttaa actgcctttt tctatattta acttagagca
     9181 taaatcagtc atacttatta ctccataatt ttttatgaaa actaatgctc ttaattgtgt
     9241 tttatttaca ttataatctg gtgcaaattt ttttaggtct tctaagtaca gactagaata
     9301 aatcttagga aatgttttta ctaaaaaact tatggtatct tttactatat agttcatata
     9361 atcacctcgg ttgttttaaa atttattaaa ttatctaaat aactttattt cagtttacat
     9421 taaaaatatt gcaagtacaa tagtttttgc tgaatgttta aatttaatta gtaatcttat
     9481 cattttttta tttttattt
//
LOCUS       DAEPOZ010000141         9451 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.141, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000141 DAEPOZ010000000
VERSION     DAEPOZ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9451
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.141"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..1807
                     /locus_tag="KR440_002535"
     CDS             <1..1807
                     /locus_tag="KR440_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBH0699573.1"
                     /translation="EDIKFTSIIILTYNQIEYTKLCIESIRKFTPKNKYEIIIIDNNS
                     SDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIELATGENILLLNNDVIVTPNWLYNL
                     NKALWSQDNIGAVGPITNACSYYQQIDVDYKSIEDMFMFAHKINTSNINLWKYRLKLV
                     GFCMLIKKSVLDNVGVLDELFTPGNFEDDDLSFRMLESGYKLLLCQDTFIHHFGSVSF
                     KENRNKYTDIMYINRRKFETKWGFNPEYSGMIRTDLISKIDDSLEANINVLEIGCGIG
                     ATLLEIKNIYKNASIYGIEISESAGKLAKYSADVLIADIEKVKLDYEKDFFDYIILGD
                     VLEHLTDPWKIVNELKQYLKKDGCLISSIPNIMHVSVLRNLVLGNFTYEDSGILDKTH
                     LRFFTLNEITKMFNDSNYIINSVTATSMPLSEIDNRFIDTMCKESCKEMRQQYESYQY
                     IVKATKSIDTKRYSSISMVNLKYALMRIDNELDVDSNLSYIFDNYNITEPCFVDDIEY
                     LVDTFVINKATLLNRLGVEAFNRGLGDFSINMFMTVLEMDRDDIDSIYNITSVLCELE
                     EFDTAYNLIKNSSKKVQSESDIQDILYFIEGKRYE"
     gene            1800..3914
                     /locus_tag="KR440_002536"
     CDS             1800..3914
                     /locus_tag="KR440_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBH0699574.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDAFIINKNFIYDILEIFKKNTDVGMIGVAGVKDI
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVANINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLDIVSKIAHFS
                     VFNHTGLYSCEKCERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAIDDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     ASQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYLESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIRRYNTSEFDRDKIKEKQSEIKHFVIDKHCIEIKNYIDKIYKEANHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            3923..4918
                     /locus_tag="KR440_002537"
     CDS             3923..4918
                     /locus_tag="KR440_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HBH0699575.1"
                     /translation="MNNIHINKFEKSCEKNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIIYKGEEILAVVPACETYENGERVFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLETYLQEQSFNKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKK
                     DILANFRYSKRRYFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            5010..6242
                     /locus_tag="KR440_002538"
     CDS             5010..6242
                     /locus_tag="KR440_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HBH0699576.1"
                     /translation="MKRLMILGSIKYFENIVMSAKKEGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKSIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAIMKKYFKNNAIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLLYPSKYCFQYEEEIKSIYQNITQAFDIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIGLYEDERQDCGRVYGIANNIFDANIKKKCI
                     HEMIEIFDENNENMIENF"
     gene            6262..7206
                     /locus_tag="KR440_002539"
     CDS             6262..7206
                     /locus_tag="KR440_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HBH0699577.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHMIGGIKMVTRLPGRNPSLNSDIFLYNMKDAKLKAILDGNYITA
                     FRTAAVAGLAIDTFAKKDFNTISIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ESFIKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYNVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLNCEEIELKEPKEKYWI"
     gene            7300..7707
                     /locus_tag="KR440_002540"
     CDS             7300..7707
                     /locus_tag="KR440_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HBH0699578.1"
                     /translation="MDKYNLIKFLSIGNEYIGNLVALESNKAIPFDIKRVYYIYDVPN
                     NVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKESIFNLHKNDVGLYIGTGVWHEMFDF
                     DEKTVLISIASEHYNEEDYIRDYKEFLKYLNNM"
     gene            7794..8885
                     /locus_tag="KR440_002541"
     CDS             7794..8885
                     /locus_tag="KR440_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HBH0699579.1"
                     /translation="MLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVRHFEEE
                     FASYVGSKYCVGLASGLDALIIAFRALGISEGDEVIVQSNTYIASVMGISINGATPVF
                     VEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASKMKDIQELCDKYNLYLIEDCAQS
                     HGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGSEKR
                     YYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKIENN
                     CTHVWHLFVLQVEKRDTFMEYLKKNGIESIIHYPIPPHLSEAYAYLGYKKGSFNIAEN
                     YSDKVVSIPLFVGMEDEEVSYIIKIINEY"
     regulatory      9194..9284
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT         3821 a          837 c         1630 g         3163 t
ORIGIN      
        1 cgaagatata aaatttacaa gcataataat attgacatat aatcaaatag aatatactaa
       61 attatgtatt gaaagtatta ggaagtttac cccaaagaat aaatatgaaa taataataat
      121 tgacaataac tcttctgatg gaacagttga gtggttaaag ggacaatctg acataaaatc
      181 tgtatataat aacaaaaacc taggatttcc aaaaggatgc aatcaaggta ttgagttggc
      241 tactggtgaa aacatattgt tgttaaataa tgatgtaata gttactccaa attggcttta
      301 taatttgaat aaagctttat ggtctcaaga taatataggt gctgtaggtc ctattacaaa
      361 tgcatgttcg tactatcaac aaattgatgt tgattataaa tcaattgagg atatgtttat
      421 gtttgcacac aaaataaata catctaatat taacctatgg aaatatagat taaagttagt
      481 tggattttgc atgttaataa aaaaatctgt gctggataat gtaggggttt tagatgaatt
      541 attcacacct ggaaattttg aagatgatga tttatctttt aggatgctag agtcaggata
      601 taagctatta ttatgccagg atacttttat acaccatttt ggaagtgtat cttttaaaga
      661 aaatcgtaat aaatatacag atataatgta tattaataga agaaaatttg aaactaagtg
      721 gggatttaat ccagaatata gtggaatgat aagaacagat ttgatttcta aaatagatga
      781 tagtttggag gcaaatatta atgttttaga aataggctgt ggtatagggg ctactctttt
      841 agaaataaaa aatatatata aaaatgcatc aatatatgga atagaaatta gtgagtcagc
      901 aggtaaactt gcaaagtata gtgcagatgt acttatagca gatatagaaa aagtaaagtt
      961 ggattatgaa aaagattttt ttgattatat aatattggga gatgtacttg aacatttaac
     1021 tgacccatgg aagatagtca atgaactgaa acaatatcta aagaaggatg gttgtttgat
     1081 atctagtata ccaaatatta tgcatgtttc tgttttaaga aaccttgtgt taggtaattt
     1141 tacatatgaa gattcaggaa ttttagacaa gactcattta aggtttttta cattaaatga
     1201 gataacaaaa atgtttaatg atagtaatta cataataaat agtgttacag ctactagtat
     1261 gccactttcg gaaattgata atagattcat tgatacaatg tgtaaggaaa gctgtaaaga
     1321 aatgagacaa cagtacgaat cttatcaata tatagtaaaa gccactaaaa gcatagatac
     1381 taaaagatat tctagtatta gtatggttaa tttaaaatac gctttaatga gaattgataa
     1441 tgaattagat gtagatagta atttaagtta tatatttgat aattataata taactgaacc
     1501 atgttttgtt gatgatattg aatatttagt tgatacgttt gtgataaata aagcaactct
     1561 attaaataga ttaggagttg aggcatttaa cagaggttta ggagatttta gtataaacat
     1621 gtttatgaca gtattagaga tggatagaga tgatattgat tcaatatata atataacaag
     1681 tgtattatgt gaattagaag aatttgatac tgcttacaac ttaattaaaa atagtagcaa
     1741 aaaagtacag agtgaaagtg acatacaaga tatactgtat tttattgagg ggaagagata
     1801 tgaataatga taagatatgt tttataacct gtgtaaatga tgaaagactt tatgaggaat
     1861 gtcttgtata tataaatagc ctagacattc ctaaaaactt tagcatagaa acagtagctt
     1921 taagaggagc cgattctatt gcctctgctt ataataaagc tatgagagaa acagattcta
     1981 agtataaggt atacttacat caagatgctt ttataattaa taaaaatttc atatatgaca
     2041 ttttagagat atttaaaaaa aatacagatg taggaatgat tggtgtagct ggagtaaaag
     2101 atatacctgt gaatggaatt tggtgggatt ctgataaaaa agcagggaaa gtatatgata
     2161 gccatacagg taaaatggat atttatgaat ttagtaactt tgaagaatgt tttaatgaag
     2221 ttatggctat agatggatta attatgatta ctcaatatga tgtattttgg agagaggata
     2281 agtttgatgg gtggcatttt tatgatttgt cacagagtat ggaatttaga caaaataaat
     2341 acaaagttat tgtaccaaaa caaaataagc cttggtgtat gcatgattct ggagtagcaa
     2401 atataaataa tggatatgat gaatatagga taaaatttgt aaaagagtac ataaatcttg
     2461 atttatatag agaaaatttt tatgaatttg ataagaagaa agcaagtacc atacttaaga
     2521 gtaataagca tgtaatggat tgtatgaaag aggagcttac agaacaatta agattaaaga
     2581 attataagga tgttttagat attgtttcta aaattgctca tttttctgtt tttaatcata
     2641 ctggcttgta ttcttgtgaa aaatgtgaga gagcattgct agaatgtgca aataatttac
     2701 cagaaataga aaatacgaaa ttatttacaa aaaaagattt aaaaagaaga aaagtattac
     2761 atgttttaag tgaaggatat tcaacaggag ggcatacaag actagttaaa aattggattg
     2821 agattgacaa agattctgtt cattcagttg taactacatg gcaatcagat acactgccag
     2881 actggttatt aaaagcaata gatgatagtg gtggcgaagt tatatctttg gatttaattt
     2941 cagatgatta tttaaaaaga gctgtagctt tgaggaaaat agcttatgac tgggctgata
     3001 tagttatttt atatgttcat atgtgggata caatacctgt tgttgctttt ggagttgatg
     3061 ggaaaatacc tattttacat gttaatcact atgatagtac tttttggtta gggacaagta
     3121 ttgcagatat tgtcattgat tttagcgaag ctagtcaatt aattacatta gataaaagag
     3181 gcatagataa atctgaaata ttaccaatgc cattaaaaga aaaagaagta ttttcaaaat
     3241 ctgaagttag aaaacgatat aatttggagg atgatgtgac aataatattg actattgcta
     3301 gtgaaagaaa atacaatagt gtaaatgata ttaattactt taaaattgct aaaacattga
     3361 ttgaaaatac agataataca attattattg ttgtaggagt tgatacagag aatctctatt
     3421 ggaaaacttt aagtaacgtt acagataata gagttatatg ttttggaaca acagcaaata
     3481 tagacgattt ttataagatt tcggatatat acctagagtc tttcatggta gggagtcata
     3541 cttcaaaatt agatgcaatt ttatatgatt taattcctgt taagtttaaa aatgaagttt
     3601 caccaatttt tacaaatatg tggaatttta tagataagtt tgattataag aatataaaag
     3661 aggttataga catgataaga aggtacaata ctagtgaatt tgacagggat aagataaaag
     3721 aaaaacaatc agaaataaaa cattttgtta tagataaaca ctgtattgaa ataaaaaact
     3781 atatagacaa aatatataaa gaggctaatc atcataaagt taagtatgat ttatataaaa
     3841 atgatgaaat cgaagactat cagctttttg ttgcattata tggttatatg gtaaaaaata
     3901 aaaacatgtc ataggtgata aaatgaacaa tattcatatc aataaatttg aaaaaagctg
     3961 tgaaaaaaac tgggattatt ttgtagaaaa taaatctata aatggtacat ttctgcaaac
     4021 tagaaatttc cttaattatc atgaagaagg aaaattcact gaccactcat taataatata
     4081 taaaggtgaa gaaatactag cagtcgtacc tgcatgtgaa acttatgaaa atggtgaaag
     4141 agtatttttt tctcatttag gaagcacttt tggaggtatt ataataagaa gtggttttaa
     4201 tgatattagg catgtaaatg ctattacaaa taagttagaa acatatttac aggaacaaag
     4261 tttcaataaa gtatatttaa aaaatacatc aagaatattt tgtaaagagg atacttcatt
     4321 actagaatat tttctattta agaataatta catgttttat gatgaattaa gtatgttttt
     4381 agaattaagt aagtataaaa aagatatatt agctaatttt agatatagta aaagaagata
     4441 ttttaaatat tctttgaaga ataatcttac ctttaaaaga ttgtatagtg atgaggatat
     4501 agtttgtttt tttaatttac tttctaattg tttagatagt aaaggagcta aagtaataca
     4561 ttcagtagaa gaacttttgg agtttaaaaa taatagatta aaagatattg tagaatttta
     4621 tgcagtatat attgatgaca ttatgatttg tgggagcatg gtatttaact ttcaagaaag
     4681 agtttatcat actcaatatc tagcatcaaa ttcgacatat aataaatatt atccaatgat
     4741 atttttatac tataatctaa taaaagatgc tttagagaga aattttaagt atctttcttt
     4801 tggaattagc actctaaata gaggcaaata cttagacgag ggtttagcta catttaaaga
     4861 aggttttggt tcaagtgcaa tttgcaacag aacatactat aaaaatataa ataagtgata
     4921 atcatgtttt atagagtttt tagagtatag taaatatcta gtactctaat attacataaa
     4981 aataattagt ttataaagaa ggtttgtata tgaaaagatt aatgatttta ggctcaatta
     5041 agtattttga gaatattgtt atgtcagcta aaaaagaggg aatatacaca attgtttgtg
     5101 ataatagaat tgatactcct gcgaaaaaga tttgtgatga agcaatagat gttgatgtat
     5161 ttgattttga taaaataaaa agtatagcta tagataaaaa gatagatgga attttaactg
     5221 gatttacaga ttctttaatg aaaccatatg tttatattgc aaatgaacta aatttaccat
     5281 gtgtgatttc ttgcaatcaa cttgaatctg taactaataa ggctattatg aaaaaatatt
     5341 ttaaaaataa tgcaatacct acaactgatt attgtattat tgaaagtcta aaagatttag
     5401 aaaaaataca aggattaaat tttccacttg taataaaacc tgttgatggt tatggttcaa
     5461 gaggtgtata ttttgtatca aatatagaag aattaataga aaaatttgaa tattctaagg
     5521 aagcctcaca agatggacaa gtaataattg aggaatttta tgaaagtgag gaaatacaag
     5581 gactagcttg ggttcataat ggagagtcac atgtatttta tattggagat agagagcttg
     5641 taaatataca tacaggaaga gctggaaaac cagatagatt actttatcca tcaaagtatt
     5701 gttttcaata tgaggaagaa ataaaatcta tatatcaaaa tataacacag gcttttgata
     5761 taaaaaatgg acctctttat gtacaaatgt tagtgggagc atatggaata aaggtatctg
     5821 aagtaatgcc aagattacct ggaggttgtg actatatggc tatttctcaa ataacagatt
     5881 tagacatagg aagtttattt gcaaacttta gtgtgaataa tgaagtaaat tttgatgaaa
     5941 taaagaaata taatatgcat ttatcaaaat gtgtatatgc attaccaata tacataagac
     6001 ctggaacaat aaaaaagata aataatatag ataaaataga aaacttagat tatgtcactc
     6061 aatttctatt attagtaaaa gaaggagata caataggatt atatgaggat gagagacagg
     6121 attgtggaag agtttatgga atagcaaata atatatttga tgcaaatata aagaagaaat
     6181 gtattcatga aatgatagaa atatttgatg aaaataatga aaatatgata gaaaattttt
     6241 aaataaggga gtatgataga tatgaagcta ataactcatg aacaaattat gtcattaaac
     6301 atttctcaga aacaaggata tgaatggata aaagaatctt ttttaacaaa aaaagattgt
     6361 caattaccac caaaaataag catggtagaa gaaggtcatg tattttacaa tgtaatgccg
     6421 tccttaaatc ataaccatat gattggtgga ataaaaatgg ttacaagatt gccagggaga
     6481 aatccaagtt taaattcaga tatatttcta tacaatatga aagatgctaa attaaaggct
     6541 attttggatg gtaattatat tacagctttt agaactgctg ctgttgctgg tttggctatt
     6601 gatacttttg ctaaaaaaga ttttaatact atttctatta tgggtttagg tgtaactgct
     6661 tctgctataa tgaatatgct ttcgtatact cttaaagata aaaatataaa tataaagtta
     6721 cttaaatata aagaccaagc agagtcattt ataaaaaagt atgaaaaaaa tgaacaattt
     6781 agttttgaaa tatgtgatac acatgaagaa cttattgtag attcagatgt agttatttct
     6841 actgtgacat atacagatga atatttatca gaatttaaat ggtttaaaaa aggtgcttta
     6901 ttattacctg tacatgttat gggatttgga aatgttgatt atctatttga taaagtatat
     6961 ttagatgatt ataatcattt gaaacatcta gaaaatatag atagatataa atattcttgt
     7021 gaattttata atgttttaag taaacaagct aaaggaagag aaaatgatga tgagagaata
     7081 atatcttaca atataggttt gtcaatacat gatatattat ttgcaaacaa aatttacgaa
     7141 ttgtgtgaaa cactaaattg tgaagagata gaattaaaag aaccaaaaga aaaatattgg
     7201 atatagttta tttcattcat atttacaata atttattgta aatatgaata tgaagtaata
     7261 tattgttaga aaattagcat gaataggata gataaattta tggataaata taatttaatt
     7321 aagttcttat ctattggaaa cgaatatata ggaaatttag ttgcattaga atcaaataaa
     7381 gcaatacctt ttgatataaa gagagtttat tacatatatg atgtaccaaa caatgtaaag
     7441 agaggatttc atgcacacaa aaacttggaa caagtactag tttgcctaaa tggaagtgtg
     7501 aagataaaat gttatgatgg tgtaaaagag agtatattta atttacataa aaatgatgtt
     7561 ggattgtata taggaacggg agtgtggcat gagatgtttg actttgatga aaaaactgtt
     7621 ttaatttcta tagcatctga acattacaat gaagaagatt atattagaga ctataaagaa
     7681 tttttaaagt atttaaataa tatgtagtta ttatacagtt agtgatgtat tttcttaatt
     7741 aaataaattt aagatagcca tatagagaat aaatagatta ggatgtgaat ttattgttgg
     7801 ttaaatttaa taatttatta aaacagttcc aaactcataa agaagaatat gaacagaaga
     7861 ttaaggatgt aatggaaagt ggatattata tccttggaaa tgaagtaagg cattttgaag
     7921 aagaatttgc tagctatgta ggtagcaaat attgtgttgg tttagccagt ggtttagatg
     7981 cacttataat tgcttttaga gcattaggaa taagtgaagg tgatgaagta atcgtgcaat
     8041 caaatacata tattgctagt gttatgggaa tcagcataaa tggagcaaca ccagtatttg
     8101 tagaaccaaa tgaatattat aatatagatt catctaagat agaagaaaag ataaatagca
     8161 aaacaaaagc tatattagta gttcatttat atggtcaagc aagtaaaatg aaagatatac
     8221 aagaattatg tgataagtat aacttgtatt taattgaaga ttgtgctcaa tctcatggag
     8281 ctaaatatga tggaaaaatg acaggaacat ttggtgatat aggttgtttt agtttttatc
     8341 caagtaaaaa tttgggtgca tttggagatg caggagctat agtaactagt aatgaggaaa
     8401 tagctgataa agtgaaaatt cttagaaact atggttcaga aaagcgatat tataataaat
     8461 ttgtcggata taattcaaga ttggacgaaa ttcaggcagg tttattaaga gtaaaactta
     8521 aatatctaaa tgaaattgaa attgaaaaat caaaaatcgt agatagatat cttaatgaaa
     8581 ttaataatcc caaagtcata cttccaaaga tagaaaataa ttgtactcat gtatggcact
     8641 tatttgtttt acaagttgaa aaacgagata catttatgga atacttaaag aaaaatggta
     8701 tagagtctat aatacattat ccgataccac cacatctatc tgaagcatat gcttatttgg
     8761 gatataaaaa ggggagtttt aatatagctg agaactattc agataaagtt gttagtatac
     8821 ctttatttgt cggtatggaa gatgaagaag ttagttatat aattaaaatt ataaatgaat
     8881 attaaactaa tcataataat atcaaaaata aattttctat atatcattta tttaaatttc
     8941 gttaattgac atttttaaat attgatatat tgtacaaata aaattgaaat atatggaaga
     9001 atatagaata aagatgagat ttgtctaata tttttataaa ttaaaaatat tttcaaagag
     9061 tttattataa aattgtagac tatagtgttt ataatctttg taattattgg taactaaatt
     9121 taaaaaataa aatatttaat tatacacaaa aaagatattt aaaaaatttg ttatttattg
     9181 tatacttaag ttaaaactaa ataggcaaat ctagagaaat ctagtgacgc aaagctatag
     9241 gggctaaggt ttgtatacac aaactatgtc agccagttgc caaaaagagt tctaggtata
     9301 ttgtatacgt aggaagagtc taggatgact gcctttttgg tggtcatttt gttatggaaa
     9361 aaatcacatt atgtagtaaa aaacacctat aataacatat tataccaaat ataatttaaa
     9421 ataaagataa attatctata aaagttacta t
//
LOCUS       DAEPOZ010000142         9382 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.142, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000142 DAEPOZ010000000
VERSION     DAEPOZ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9382
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.142"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            320..1222
                     /gene="ptb"
                     /locus_tag="KR440_002542"
     CDS             320..1222
                     /gene="ptb"
                     /locus_tag="KR440_002542"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HBH0699580.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            1244..2323
                     /gene="buk"
                     /locus_tag="KR440_002543"
     CDS             1244..2323
                     /gene="buk"
                     /locus_tag="KR440_002543"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBH0699581.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            2313..2990
                     /locus_tag="KR440_002544"
     CDS             2313..2990
                     /locus_tag="KR440_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0699582.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            3033..3248
                     /locus_tag="KR440_002545"
     CDS             3033..3248
                     /locus_tag="KR440_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0699583.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            3277..4356
                     /locus_tag="KR440_002546"
     CDS             3277..4356
                     /locus_tag="KR440_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBH0699584.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            4356..5108
                     /locus_tag="KR440_002547"
     CDS             4356..5108
                     /locus_tag="KR440_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBH0699585.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            5109..5666
                     /locus_tag="KR440_002548"
     CDS             5109..5666
                     /locus_tag="KR440_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBH0699586.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            5836..7182
                     /locus_tag="KR440_002549"
     CDS             5836..7182
                     /locus_tag="KR440_002549"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBH0699587.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILNGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            7449..9281
                     /gene="glmS"
                     /locus_tag="KR440_002550"
     CDS             7449..9281
                     /gene="glmS"
                     /locus_tag="KR440_002550"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBH0699588.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEEEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
BASE COUNT         3637 a         1132 c         1925 g         2688 t
ORIGIN      
        1 tgttgaagtg gaatgaacaa atatttcgaa tatttagttg ttacaagatg cgacaaaaaa
       61 acctataaaa ttaagtttgt taattttatg tttttgttgc atcttgttta ttttagttat
      121 attataaaat aaggacaatg agatatttgt taaatctaaa aagcgctaga tatatgaaaa
      181 aagactgata aatagattta aataaatatt ttgaattaaa tatttcaatt atctctagga
      241 aaacgttgct gaacgagtta taatttaatt gagatataaa aagttctatt tcaaaaaatt
      301 caagatgagg tggaaatgca tgagaagttt tgaagaagta attaagtttg caaaagaaag
      361 aggacctaaa actatatcag tagcatgttg ccaagataaa gaagttttaa tggcagttga
      421 aatggctaga aaagaaaaaa tagcaaatgc cattttagta ggagatatag aaaagactaa
      481 agaaattgca aaaagcatag acatggatat cgaaaattat gaactgatag atataaaaga
      541 tttagcagaa gcatctctaa aatctgttga attagtttca caaggaaaag ccgacatggt
      601 aatgaaaggc ttagtagaca catcaataat actaaaagca gttttaaata aagaagtagg
      661 tcttagaact ggaaatgtat taagtcacgt agcagtattt gatgtagagg gatatgatag
      721 attatttttc gtaactgacg cagctatgaa cttagctcct gatacaaata ctaaaaagca
      781 aatcatagaa aatgcttgca cagtagcaca ttcattagat ataagtgaac caaaagttgc
      841 tgcaatatgc gcaaaagaaa aagtaaatcc aaaaatgaaa gatacagttg aagctaaaga
      901 actagaagaa atgtatgaaa gaggagaaat caaaggttgt atggttggtg gaccttttgc
      961 aattgataat gcagtatctt tagaagcagc taaacataaa ggtataaatc atcctgtagc
     1021 aggaagagct gatatattat tagccccaga tattgaaggt ggtaacatat tatataaagc
     1081 tttagtattc ttctcaaaat caaaaaatgc aggagttata gttggggcta aagcaccaat
     1141 aatattaact tctagagcag acagtgaaga aactaaacta aactcaatag ctttaggtgt
     1201 tttaatggca gcaaaggcat aattttaagg gggaaatagg caaatgagca aaatatttaa
     1261 aatcttaaca ataaatcctg gttcgacatc aactaaaata gctgtatttg ataatgagga
     1321 tttagtattt gaaaaaactt taagacattc ttcagaagaa ataggaaaat atgagaaggt
     1381 gtctgaccaa tttgaatttc gtaaacaagt aatagaagaa gctctaaaag aaggtggagt
     1441 aaaaacatct gaattagatg ctgtagtagg tagaggagga cttcttaaac ctataaaagg
     1501 tggtacttat tcagtaagtg ctgctatgat tgaagattta aaagtgggag ttttaggaga
     1561 acacgcttca aatctaggtg gaataatagc aaaacaaata ggtgaagaag taaatgttcc
     1621 ttcatacata gtagaccctg ttgttgtaga tgaattagaa gatgttgcta gaatttctgg
     1681 tatgcctgaa ataagtagag caagtgtagt acatgcttta aatcaaaagg caatagcaag
     1741 aagatatgct agagaaataa acaagaaata tgaagatata aatcttatag ttgcacacat
     1801 gggtggagga gtttctgttg gagctcataa aaatggtaaa atagtagatg ttgcaaacgc
     1861 attagatgga gaaggacctt tctctccaga aagaagtggt ggactaccag taggtgcatt
     1921 agtaaaaatg tgctttagtg gaaaatatac tcaagatgaa attaaaaaga aaataaaagg
     1981 taatggcgga ttagttgcat acttaaacac taatgatgct agagaagttg aagaaagaat
     2041 tgaagctggc gatgaaaaag ctaaattagt atatgaagct atggcatatc aaatctctaa
     2101 agaaatagga gctagtgctg cagttcttaa gggagatgta aaagcaatat tattaactgg
     2161 tggaatcgca tattcaaaaa tgtttacaga aatgattgca gatagagtta aatttatagc
     2221 agatgtaaaa gtttatccag gtgaagatga aatgattgca ttagctcaag gtggacttag
     2281 agttttaact ggtgaagaag aggctcaagt ttatgataac taatgataaa agaataagaa
     2341 tcataactgg tcactatgga agtggtaaaa gtgaatttgc gatgaactat gttgttaagt
     2401 taagagatat ggtttctggc aaagttgcta tagccgattt agatgtggta aatgtatact
     2461 ttagaactag agaaaagaaa gagttaatga aatccttagg tatacaacca atagatagct
     2521 caattaatgc tccaacatta gatttaccag cagtatcagc agaggtaatg tctcctatgg
     2581 tagaccattc atacaattct gttatagatt taggtggaga taatgtaggt gctagggtta
     2641 taggaagatt tagtcatttg ttaaaagaag gcgattatga tatgcttttt gtaataaatg
     2701 caaataggga gaaaactcaa acttctgaag aagttatcca atatataaaa gaaatagaaa
     2761 aatcttctaa attaaaggta acaggtctaa ttaataatac tcatcttatt aggtttacta
     2821 ctattgatga tgttttaaga ggtcagaaag ttgcaaaaga agtttctgaa aaatgtaata
     2881 taccgataag atatgttgca tgtttagaaa atcttgttga acagttacca aaagatttag
     2941 aaggagaaat ttttcctata aaattatata tgagagaaga ctggatgtag tttgagctca
     3001 aatatataga aagtttaagg aggcttaggt aaatggctaa aggaaaagta tcttttaatc
     3061 aagaacgttg taaaggttgt ggattgtgtg ttgaagcatg tccagtaaaa atcatacaat
     3121 tagactcaaa tgtaataaac aaaaaagggt ataacccagc aactgttttt gaaatggaaa
     3181 aatgcatagg atgtgcaagt tgtgcaacta tgtgtccaga tgtagttata acagtagaaa
     3241 gagattaatt taattaatat ttaaggggga attttaatgg ctaagatact tatgaaaggt
     3301 aacgaagcat ttggaaaagc agctatagaa gctggttgca aatatttctt cggttaccca
     3361 ataactccac aaagtgaatt accagaatat ttatcaagag agttacctaa aataggtgga
     3421 gcgttcgttc aagctgaatc agaagtttct gcgataaaca tggtttatgg tggagcaggt
     3481 gcaggagcaa gagttatgac ttcttcatct tcaccaggag ttgctttaaa gcaagaaggt
     3541 ataacatatg cagtaggagc agaagttcct tgtgttgtac ttaatgtaat gagaggtggt
     3601 ccaggacttg gaagtataca accttcacaa gctgactact ttatgtctac aagaggtgga
     3661 ggaaatggag actacagaac tccagtattc gcaccagcta cagttcaaga agctgttgat
     3721 atgataatgg aagcttttga cgttgcagat tattacagat caccagttat ggtagttgca
     3781 gatggtatga taggtcaaat gatggagcca gttgaattta gagctcctga gaagaaaaga
     3841 gaattgcctc ctaaagattg ggcaactgtt ggaactaaag gaaaaagaaa acctaatgta
     3901 ataaactctc tatacttaga accagaagta ctagaagacc attgttggca tttacaagaa
     3961 aaatttgatg caatggaaaa gaatgaagta caatacgaaa tgtacaaaac agaagatgct
     4021 gaatttgtat ttgcagctta tggaactact tcaagagtag taaaaagcgc aattgatata
     4081 ttaagagaag aaggaataaa agcaggtctt ataagaccta aagtattatg gccattccca
     4141 ttcgaagcat tcaaccaaat tcctaatgct agaaacatat tgactgttga aatgagtatg
     4201 ggacaaatgg tggaagatgt taaaatggca gtagaaggta aattaccagt ttacttccat
     4261 ggaagaccag gaggaatgac tccaacacca gcagaaatag ttgaaaaagc taaaaaaatt
     4321 attgccggag aattggttgc aggaggtgct agataatggc tgttgtattt aagaaaactg
     4381 aaggattaca agatactcaa actcactact gtccaggatg tacacatggt ataatccata
     4441 gattagttgg tgaagtatta gaagaactag gagtattagg tgatgctgta ggtgttgttc
     4501 cagttggatg ttcagtttta ggatataaat attttaactg tgacactcaa gaagctgctc
     4561 atggtagagc gccagctgct gctacaggaa taaaaagagt tcatcctgaa aatacagtat
     4621 tcacttatca aggtgatgga gacttagctt caattggtac tgctgaaata gttcatgcag
     4681 ctgctagagg agaaaaaata actactatat ttgtaaacaa tacaacttat ggtatgactg
     4741 gtggacaaat ggctccaact acattagttg gacaaagagc tactactgct caatcaggaa
     4801 gaaatgctga aactcaaggt tatccaataa gagtaagtga aatgttagct acattaacag
     4861 gagctgtatt tgtagaaaga gtagcagttg atactccagc tcatgttaga caagctaaaa
     4921 aagctataaa gaaagcattc caagttcaac aagcaggatt aggatttgga atagtagaag
     4981 tattatctac ttgtcctaca aactggggac tagctccaaa cgatgcttta caatggttaa
     5041 gagacaacat gataccatac tatccattag gaaatttcaa aaatgttgaa gttgaggagg
     5101 tgaagtaaat gtctacagct agagtaatat gtgcaggctt cggaggccaa ggtgttatgt
     5161 ctatgggaca attacttact tatgcaggaa tgctagaagg aaaagaagtt tcttggttac
     5221 catcttatgg accagaaatg cgtggaggta cagcaaactg tgcagtaact gtatctaatg
     5281 agccagttgg ttctccactt ataacagatg atgcaactgc agctatatta ttaaatatac
     5341 cagcatttga aaaattcaaa gatgatgtta ttcctggtgg aaaaataata gtaaatagtt
     5401 ctttaataaa agaaaaagtt gatagaacag atgtagatgt ttattatata ccagcaaatg
     5461 aattagcagc tgaattagga aatgataaag ttgctaatat gataatgtta ggcgcttact
     5521 taaaagtatc tgatactgta gatatagaat ctgtactaga agcattcaaa aaagttttcg
     5581 gacctagaaa agaaaaattc gttccattaa acagagaagc tttacaaaaa ggtatggatg
     5641 ctacatgttc taaagcaact aactaataaa gaaaaagatg tcaaaaaaaa gacaatagaa
     5701 ttatttctat tgtctttttt gctgataagt attaaaatgt ttttatgact gaaattaagc
     5761 ctataatgat attttatgaa ctataaaaat ataatacaaa ttacaagctt ttcaattatg
     5821 aaaggagaat ctttagtgag aaagtatttt ggaacagatg gagttagagg agtagcaaat
     5881 acagaactaa catgtgattt agcatataaa cttggtagag ctggtggatt tgtcttagct
     5941 caaggagacc acagagtaaa agtagtagta ggtaaggata ctagaatatc aggtgatatg
     6001 ttggaagcat ctttaatagc aggacttatg tctgttggat gtgatgttat tactgttgga
     6061 ataataccaa cacctgcagt ggcatattta actagaaaat atggtgctga ttgtggtgtg
     6121 gttatatctg catctcacaa tcctgttgaa tataatggaa tcaaattctt taataagaat
     6181 ggatataaat tagatgatga aattgaattg aaaatagaag aatatataga cgatatagac
     6241 aaaattgatt gtcttccaat tggtgaaaat gtaggaagaa aattacatga acattgtgca
     6301 caaagagact atgtagacta cctaaaatca ataataagca ctgactttaa gggattaaaa
     6361 gtagttttgg actgtgctaa tggtgcgtca tataaagtgg caccaatagt gtttgatgaa
     6421 ctaggagcaa gtgtaatttc aataaatagt tcaccagatg gaaataatat aaactataaa
     6481 tgtggctcta cacatccaga acaacttcaa agggctgttt tggaacataa tgctgactta
     6541 ggtcttgcat atgatggaga tgctgataga ttaattgcag taaatgaaaa gggtcaaatt
     6601 gttgatggag accatataat gatattgagt gccttaaatt taaagaaaaa taataaattg
     6661 gcgcaagata ctttggtagt tacagttatg agtaatatag ggttgacaat agctgctaag
     6721 gaaaatggaa taaatttatc tactacagca gttggagaca gatatgtttt agaagatatg
     6781 gttaaaaatg gatataatct tggtggagag caatctggac atatgatatt tttagattac
     6841 aacactactg gagatggagt gcttagttct ctaatacttg caaatataat cttgcaagaa
     6901 aagaaacctt tatcagaaat tgcatcaata atgagccaat acccacaagt attagtaaat
     6961 gcaacaataa aaaatgaaaa taagaacaaa tatatggaat atccagaaat aaaaactgag
     7021 atagaaagaa tagaatctat tttaaacgga aatggtagag ttttgataag accatcagga
     7081 actgagccat tagttagagt tatgttagag ggtaaagagg aaggtcaaat aaaagaatta
     7141 gcaacaaact tagctaatct tatacaagaa aaattatcgt aatttaaagg gtattctcag
     7201 atacaaatac tgaggacacc tttctaagcg ccagaactta gttatagaca taattattga
     7261 gtaatatagg aactaagttg acgaggtcag agttaatcga attgtcggcg gatgctctgc
     7321 ggtgtgttta ccatcgaaag acttctttaa ataataggag caatcctatg gacaaaggga
     7381 agccaaagta aacttatatg acctaactct taaaatatat aaataatatt tttaggagga
     7441 cacaaataat gtgtggaata gttggatatt taggaagtag aaaggcagca gaagttatag
     7501 tagaagggct ttctaagcta gagtacagag gttatgactc agcaggagta gcagttaata
     7561 gttctaatga agaagaactt aatattagaa agtttaaagg tagactttct gtacttgcag
     7621 aagacttaga aaagaatcct attgatggaa atttaggtat agggcatacg agatgggcaa
     7681 ctcatggaga accatctgat gtaaactcac atcctcattt taatcaagcc aaaacaatag
     7741 cagttgttca taatgggata atagaaaact atatggaaat aaaagaagaa ttaatttctg
     7801 aaggtgttaa gtttgaatct caaacagata cagaggtaat agcacattta gtagataaat
     7861 actatgaggg taatttatta gatgctgtat acaaaactat atcaaaatta agaggagctt
     7921 atgcattagg tgttatatgt aaagaacatg gaaatgaatt agttgcagta agaaaagata
     7981 gtcctcttgt agttggtgtt ggtgaaggtg aaaactttat agcatcagat atacctgcat
     8041 tattaaaata tactagagat gtttatttct tagaaaatgg agaagtagtt catctaaaag
     8101 acgaaaatgt aacagtttat gactctaata gaaatcttgt tgaaaaagaa gtatttcatg
     8161 taacttggga tgtagaagct gcgtctaaag gtggatatga ctactttatg tcaaaagaaa
     8221 ttcatgagca accaacagga gttagagaaa ctttagaaag aagacttgat gataatggaa
     8281 atataattct agacagtata aatatttcta aagaagactt agaaaaaata aataaagttt
     8341 atatagtggc ttgtggaaca gcatataatg ctggattact tggtaagtat gccatagaaa
     8401 aatttgtaaa tataccagta ataactgata tagcatcaga atttagatat agcgacccat
     8461 ttgtagatga aaattcatta gtaatattag ttagccaatc tggtgagaca gcagatactc
     8521 tagcagtatt aagagattca aaggctaaag gagcaagaat actttcaata actaacgttg
     8581 taggttcatc tatagcaaga gaatcagatg atgtattcta tacttgggca ggacctgaag
     8641 tagcagttgc atcaactaag gcatatacta ctcaaataac ttctttatac atgatagctt
     8701 tagactttgc tatcaaaaaa ggaacaataa ctagagaatt ttatgatagt atgatttcta
     8761 agatgaaaga aataccatca aaaatacaag aaatattaga caatgaagaa tatataaaag
     8821 aagttgctaa aacagttgtt agttctgaac atgcattcta tttaggaaga ggtatagact
     8881 atagcttagc aatggaaggt tcattgaaat taaaagagat ttcatatata catgcagaag
     8941 cttttgcagc aggagagcta aagcatggaa caatagcttt aatagaaaaa ggaactcctg
     9001 taattgccat agctactcaa gaaaagttat ttgaaaaaat ggtttctaat atggaagaag
     9061 taagagcaag aggagcttat gtagtagcaa tagctcaaag ccataataag gatgttgaga
     9121 aagctgctga taaaataatc tatataccaa attcagatga tattttatca ccaatattag
     9181 cagttgtacc aatgcaatta cttgcgtatc atgtctctgt actaagagga tgcgatgtag
     9241 ataagccaag aaatctagct aaatcagtaa cagttgaata aaaacagatt aaaaattaaa
     9301 tagataatat ttattgaaaa aaactcatcc tgttatttgt attttatagg atgagttttt
     9361 tagataaata ttaaatttgt tt
//
LOCUS       DAEPOZ010000143         9269 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.143, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000143 DAEPOZ010000000
VERSION     DAEPOZ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9269
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.143"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(541..3648)
                     /locus_tag="KR440_002551"
     CDS             complement(541..3648)
                     /locus_tag="KR440_002551"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBH0699589.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(3741..3973)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4148..4675)
                     /locus_tag="KR440_002552"
     CDS             complement(4148..4675)
                     /locus_tag="KR440_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBH0699590.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(4710..5495)
                     /locus_tag="KR440_002553"
     CDS             complement(4710..5495)
                     /locus_tag="KR440_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBH0699591.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(5505..5762)
                     /locus_tag="KR440_002554"
     CDS             complement(5505..5762)
                     /locus_tag="KR440_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBH0699592.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFNILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(5781..6239)
                     /locus_tag="KR440_002555"
     CDS             complement(5781..6239)
                     /locus_tag="KR440_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBH0699593.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(6279..6983)
                     /locus_tag="KR440_002556"
     CDS             complement(6279..6983)
                     /locus_tag="KR440_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBH0699594.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(6993..7628)
                     /locus_tag="KR440_002557"
     CDS             complement(6993..7628)
                     /locus_tag="KR440_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699595.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IPVKGTIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            8009..9049
                     /locus_tag="KR440_002558"
     CDS             8009..9049
                     /locus_tag="KR440_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0699596.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGIITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
BASE COUNT         2787 a         1633 c         1052 g         3797 t
ORIGIN      
        1 taccatctaa ccaatttttt ctactttcgt aactttcaca cttaggttta tttcatcctt
       61 atgttttagt ttggttagct ttaggggttt ccagcaattc aagtagtatt ggataggttc
      121 tatcctatct ctacatatag gtttccctat atgctgacta ttttagctac taactcatga
      181 ctaaattcac gagtgtgcgt agctattttt taatcaatac ttaacttttt gttttcacca
      241 tacatcaaaa cgcacccctt tgcataataa ataaaccacc ctgcagaaca cacgataaat
      301 ttataaataa atttatcaga agtagcttac taaaaatatt attcatcctc acttaaacgt
      361 ttattcatta atattctatc acatgacata aataatttat atgatttata tagatttatt
      421 tattgtaaat tttggaatat taaaacattt tattgaatag ataattatat tacataaaaa
      481 atccctatag aaatctataa actaatttct atagggattt tggttaacta actattcttt
      541 ttattttttt ataactttta tacctgtcat atggtcattt aagttttgtt cctctaaaga
      601 tttatcatct actctgttta tttctaaagc taaagtttca gacttgatat attcattaaa
      661 atggtcaaca gccttagcaa tttcatcatc accacagtat tctattacta tattatctaa
      721 tacttcaaag ttattagatt ttcttaattg ctgtacttta gatataaatt ctctagcata
      781 accttcatta atcaactctt cagttagctt agtatctagt ataacaaaaa gattgttttc
      841 aacagataca ttaaatcctt ccttagcagt tatacctatt aatactaaat ccttattaaa
      901 ttcaaaagct tcgccatcta aatcaactgt taaagtctca cctttttcaa gtttattagc
      961 agtttcatga gcatttaatt tacttaatgc tccaacgaat aaattcatct tagaacctaa
     1021 taatggccct gcttccttaa agtttggttt taaactaaag ttcatatact catctaaatc
     1081 cttagcaaat attacctctt taacattaag ctcttctttt attaaatcaa ctacatcact
     1141 tattgtatct tcaaacttac catctactaa cactttttgt aatggttgac gaacttttat
     1201 tctttctact tctcttgaag ctctacctaa agtaactaag tttttagcta aatccatttt
     1261 ttcttctaat ttagtatcta ctaactcttc attacattta ggatattcag ctaaatgaac
     1321 agatacttct cctgttaagt ttctatacat ttcttctgac atatatggtg caaatggagc
     1381 tatagctcta caaagaccat gtaacaattc ataagttgta ttgtacactg cttttttatc
     1441 ttctgttaat tcagttgccc agaatcttct tcttgaacgt cttatatacc agttagataa
     1501 atcatcattt ataaaatctt gtatcatcct taaagtttta tttaactcaa atatagctaa
     1561 attttcttct acatctttca ttaaattatt gaacttagat aatatccatc tatcaagttc
     1621 tggtctatct ttatattcta caaaaaattc agttggattt atatcatctg tgtttgcata
     1681 taaagtaaag aagttatata cattcttcat agtacctata aatttacttt ggatttcttt
     1741 taatccatcc atatcaaatt tagttggagt ccatggtgga gaaacatata gtaaatacca
     1801 tcttaatgca tctgctccat attggtcaaa caattccatt ggatttactg tatttcctct
     1861 tgatttactc attttcttac catctttatc aagtacaagg tctggaacta aaacactctt
     1921 atatggtgct tttcctaata caaatgttga tactgcaagt aatgagtaga accaacctct
     1981 agtctggtct ataccttctg atatatagtc agctgggaac aattggtcaa aattttcctt
     2041 attttcaaat ggatagtggt gttgagcaaa tggcatagcc ccactatcaa accaacagtc
     2101 tataacctct gttactcttg tcattggttt tccacatttt tcacatttta agtgtatatc
     2161 atctacatat ggtctatgaa gttcaactgt ttctggattt acatcttcta tagctttttc
     2221 agctaattca gctcttgaac caactgaatc tgtatgacca cattcacatc tccatatagg
     2281 aagtggagtt ccccaatatc tagttcttga tattgcccag tcatttagat tttctaacca
     2341 gtttccaaat ctcttctctc ctacaaaact tgggaaccat tttacaccat tattattttc
     2401 tattaattta tctttaagtt tagtcatctc tatataccag cttggttttg catagtatac
     2461 taatggagtt tgacatctcc agcagtgtgg gtaattatga actacttttt ctttgctata
     2521 taatttattt tcagaagcta gccattttat tatttcaaca tctagcccat cttccattac
     2581 aaatctacct tcccaaggag tagatgtaaa tttaccagtt tcatctactg gttgaataac
     2641 tggtaaatca tatcttttac ctgtgttgta gtcatcttca ccaaatgctg gtgctgtatg
     2701 aactatacca gttccatctt cagtagttac ataatctcct actgtaataa aaaatgcttt
     2761 tttgtcaact tctacaaaag gcattaattg ttcatattct aaaccttcta ggtctttacc
     2821 tttcatttct tctaaaactt cataatcttc acctagtact ttatcagcta aaggtttaga
     2881 tacataatat acctcatcac cttttttaac ttttaaataa tctacatcag catttactgt
     2941 taatgaaaca tttgatggta aagtccatgg agttgttgtc catgctaaga agtattcatc
     3001 agcatctttt ttcttaaatt tagctataac tgtattagtt tttacttctt tatatccttg
     3061 tgcaacctca tgactagcaa gaccagttcc acatcttggg caataaggaa gtattttatg
     3121 cccttcatat atatatcctt ctttgttgaa tttgtttagt atccaccata cagactctat
     3181 atagttatta tctaaagtta tatatggatt atctaaatca acttcataag ccattctctc
     3241 agtcattatt ctccattgtt tttcaaatga gaaaactgac tctctacatt tttggttaaa
     3301 ttcagctatt ccatattctt ctatttgttg cttgctagta agacctaact ctttctctac
     3361 ttgtatttca actggtaatc catgtgtatc ccaaccagct tttctcttta cttgataacc
     3421 agacattgtt ttatatctac aaactgaatc tttaagcgtt ctagataaaa catggtgtac
     3481 tccaggattt ccattagctg ttggaggtcc ttcatagaat acgaaacttg ggtcatcttt
     3541 ccctttttct aaagtttttt caagtatatt tatgtctttc caataatcaa aaacttgtgc
     3601 ttctgcttgt tttacagaac tgtctaccaa tggcttaaat ttcgccatgt aaatcaccct
     3661 ttcctactaa atattataat tccaaaattt tttattcttt cacataaatt taaatactaa
     3721 aaaactcgcc ccctttaaac caaagggacg agttataaat cgcgttacca ccctaattct
     3781 atatgtaaac taaatactag ctacatataa cactctaata gtataacggt actaaccggc
     3841 acagcttagt agaatttaat cgttcagcct gcagctcaag agtgattttt atctttataa
     3901 attcattggt tctcagcata cccaattctc tgtaaaatta tctaaagata aatgtctcta
     3961 tcaacgcttt taaaaattta tctgtgttta tatgaaattt tataacaaat aatttgaata
     4021 gtcaatatat ttttgaaaaa tataataatt tctatttttc ttacattttt atcctatttt
     4081 taatctttgt tatagatttc tcaaactcac acagttattc tacataaaaa ataaacaaat
     4141 tacaagctta ttctaaagtt gtagcagctt catcattact ataattatac actgttgttc
     4201 cttcaaatgc atttacacat ttttcatcta catcataaaa aatctcatca acacttctta
     4261 tttcatcttc taacagtgat ttaaatttat ttctaaatat cttaaattct ttgactaaag
     4321 catcatactc ttttcttatc tctatgacac ggttattagc ttgttctata atttgtctag
     4381 cttttaaatc agcttcttca acaattatct tagccttttt attagccgcg ctacaagtgt
     4441 cttctgctgc actttgagca gttattaaag tagcttttag tgtttcttca atattttcat
     4501 acttgtttac ttggtcttga tataacccta atttttcttt aagagctgta ttttccctac
     4561 atagagattc ataatcttcc ttgatgatat ctaaaaattc atcaacctct tcatctctat
     4621 accctcttaa acctttttta aactctttat tttctatctc aattggagtt agcattacat
     4681 ttcctcccag ctataattta aattagtcct tatacaatta atcttgcttg aacttttatt
     4741 tttccttttt tggtaatatc tcctatttgt gttaccttag ctcttccttt gcctctaata
     4801 gaaatcaaat catcattttt tactatttta gaagttgaag ttatcttttc ataattgaca
     4861 tggacttttt ctccatttat gtattttgca ctatcttgtc tagatatatt gtaaatacca
     4921 cttatcacac aatctaacct atctgaagat actgtaaatg atacttcttt atattttgaa
     4981 gaactattaa caatattttc tctggaaatt tcctttacaa ttacattatt tcttgaaacc
     5041 ttttctaagt ttacaattat aaaatcgcaa atatcactac ttacaactac ttgacaaaag
     5101 ctatcatgta taattatatc tccaattttt tctcttttta tacctagacc cagtattgac
     5161 cctaagtaat ctttatgaga tatattttta aacttgaaat tcccttctat ttgtaaaaat
     5221 ctaagagtat cttctatatc ttcatattcc atgtaaaatg gataaataaa tacagtgctt
     5281 ctttcagctt gttcataacc tccatccaca ctgtatttta tttcatttgt agaatttaat
     5341 attgctacag catttttcac ttcatatgga ttaagaaact ccgtagactt aacatcatag
     5401 ttttttatac aactattagc cttgtctatc actttaaaca ttttattttt caagtctata
     5461 tcctttatgt gatttgttag ttttaattta tccatcctat caccttagaa tataaccaat
     5521 actaattgtc ttaggaacat caataatact attgcaatta aaggagaaaa atctataggt
     5581 ccatttgtat atttatacat tatagacctt attggccctt ctattggctc tgtaagattt
     5641 tccaaaatat taaaaatttt actttgtctt ccatttggaa accaagtcat caagcttttt
     5701 ataactatca tccatgctat tatatctaaa agataataaa gtgcaattct tattgttccc
     5761 atatattaac cgccttttta ctatttattt tgccaaggga agaatgcttt actttctaat
     5821 tcttttttca tatttgcatc tatatccaca ttattaggag ctagtataaa tattgattta
     5881 gaaacctttt gtatacttcc atctaataca taaactgccc cattcataaa gttaaatata
     5941 gatttacgaa cttcagaatt agtcaatcct tccaaattca ctataactgc ccttctttgt
     6001 tttagatggt ctgctatagt tgcagcttca tcataaacct taggttcaac tattacaact
     6061 ttcatttggc tagttgtatg taaatttaca attttatttg cttttgcagt actaaagcca
     6121 ctgttcattt cttcttcttg aactatatcg tccatgccac tttcatattc atcttcaata
     6181 taatcttctt cttcatcaac tatccaattc ttaaatttag atattattcc atttgacatt
     6241 attaacttcc tccttaaaat ttattcaaat ttgagtaatt attttttatt gtagtttctt
     6301 tctccaaaga ttgatgttcc aactctaaca atggtagccc cttcctctat agcaatttca
     6361 taatcgtgac tcattcccat tgacaaaaca tccatcccta cattagggat gtttagttct
     6421 tttatttgtt ttgataaatc tttaagacct ttaaatacac ctcttatctc atcttcatct
     6481 tctataaaag gtgccattgt cattagaccc tttattttta tatttttata tttttctgac
     6541 acttctttga caaaatctac aacttcttct ctatcaagtc catgcttaga ctcttcttta
     6601 gatatattca cttgtactaa gcaatctatt tctctgttta tttgttctgc acgtttttga
     6661 atttcttcgc ataatgcaat tctatctaaa gagtgtatca tacaaacttt atcgattatg
     6721 tacttaactt tattggtttg caaagttcct atcaggtgcc agtttaattt gtcacctata
     6781 atatcatact ttctagctaa ttcttggggt ttattttcac caacagaagt gattccttcc
     6841 ctcattgcct cattaactaa gtctacatca acagtctttg tgactgccaa aagggtaatc
     6901 tctttgctgt ctctatctac tttttttgca gctttatcaa ttttgtttct tatctcagac
     6961 aaattatctt taatagaacc cattattatc acctacttta tccttatatt tgtgtttata
     7021 ctgttaggtc ttaaaattat tttgtcatat agattaacac ttgtaactcc atttatgtca
     7081 tttttataat aatccacaaa tacagaagat tcattctcat aagttattcc ttctaatatg
     7141 acaaatttta cactttgtga ttgttcatca attacatata caccacgttt attgtctttt
     7201 gtttttatag aagttttagg tatttttaaa cattccatct gattatatat tatatcaaat
     7261 tcttcgacac ttgtatcctg atttgcaaca atttggttac ttatttttaa ttttaccatt
     7321 attttattat tattttcata tattttgtaa acttttgcat ctaatttttg ctcatgatat
     7381 tcaattatta cattttgatt tttttcaaag ttattagaat cttctttact gatgtatgta
     7441 gatagataat tactttggtc attggcatct ttttctatag ttccttttac aggaatcttt
     7501 gcatcaactt tttcattttt taacaccaac gtatctacat ttttgctaat tatcattact
     7561 aatatattaa atactaaata tataagaata ccaagtatca ctaatctggt atattttacc
     7621 tttttcaaag actctccccc tataaaatta ctttcattat aagtacttct tctaaaaaat
     7681 gtttattcct tttttataag ctaatatttt acgttctaat aattattttt tccatattaa
     7741 atttattttt tatcaatctt cctatacaag actgcattat ttatatataa ttgtattata
     7801 aagtataagt ttaatttaca ttttatacat taagcttact ttatatgttt ttatagttat
     7861 ttttattttt agatgcaata taaaaccaat taagatttaa aataactttt gttatgttga
     7921 catttattta ggtaatcatt tacgaatcgg gataacgtta tcataacaaa agtttatgtc
     7981 ctaaaaaaaa tacaaggagg attttcttat gtcagaaatt ttattaggaa caggtttact
     8041 tttattggtt ctatgttgtt tcacaatttt tagttacaag gccccatatg gtatgaaggc
     8101 tatgggagct ctagcaaacg ctgcatgtgc tactttctta gtagaggcat tcaacctttc
     8161 tttacttggt gatgttttag gtatggaatt ttttagaagt gttggagctg ctaatggtag
     8221 tcttggtgga gttgctgctg ctatacttgt gcctttagcc ttaggtgtat ctccagttta
     8281 tgcagtttta ataggattat cttgtttaga tttttctata ttatctggat tcgttgctgg
     8341 atactttgtt tctttcttaa ttaagtttat agagaaaaaa gtgccacctg gagtggattt
     8401 agtggttgta attcttctat gtgcaccgct atctcgtctt atagcatcaa atgtagaccc
     8461 attagttaat agtacactac tacaaattgg acaagttttg atagaagcta ccaacattag
     8521 ccctataata atgggaatta tactaggagg cataataaca gtagttggaa cgtctccact
     8581 tagttcaatg gctcttacat ctataattgg tcttactggt cttcctatgg caataggagc
     8641 attaggtgct tttggttcag cttttatgaa ctcaacacta tttgcaaaat tgaaattagg
     8701 ttctaaaaaa gatattatat ctgttgctat tgaaccatta acccaatcag acataatttc
     8761 agctaatcca atacctatat attctactaa tttctttgga ggagcctttt ctggtatcat
     8821 tgtagccttg atgggtcttg taaacatgac accaggtaca gctacacctg tggctggttt
     8881 tgctataatg tttgcttata atccagttca acaagttctt ataacagctg gtttatgtgc
     8941 tttattcaat gtaattgcag gatttttagg ttcaaaaata ttcagtacct ttaaagttct
     9001 tactgctggt gaaatacgtg gtactgatgt agaagaagaa attgcttaaa aaacaataag
     9061 atttataaat ttatatctca agtagtagag ttttatattg taggcaattt tattaattgc
     9121 aaacgtttta ttttttattt atgtttatga ttattatgtg tgttgaatat ttagataaat
     9181 ttttatttat tatttttatt tagatttttg tttagttttt tatttggttg atatattagg
     9241 aataaaaatt agtatagttt attattagt
//
LOCUS       DAEPOZ010000144         9263 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.144, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000144 DAEPOZ010000000
VERSION     DAEPOZ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9263
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.144"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            70..345
                     /gene="flgM"
                     /locus_tag="KR440_002559"
     CDS             70..345
                     /gene="flgM"
                     /locus_tag="KR440_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HBH0699597.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGVKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            350..766
                     /locus_tag="KR440_002560"
     CDS             350..766
                     /locus_tag="KR440_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HBH0699598.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGDDISMSEVVENSNDRYLKEIYTDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKQTATYNSYGNIRK"
     gene            784..2094
                     /gene="flgK"
                     /locus_tag="KR440_002561"
     CDS             784..2094
                     /gene="flgK"
                     /locus_tag="KR440_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HBH0699599.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGNTVSKYDYY
                     NTIETIINEPSDYGISAGIDDFFKGWESLAKDINSDSKKSLVVENASNLATLISESYK
                     KLNKLKGDIDDNINSEIKQINDMLKSLEDLNKSIDVISGSGSTPNDLLDERDRILDDL
                     SFKLDLENSDVKNMLSDGKLELNELKNADGTWKTGVSGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKVNPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESVGLDSQKVNQDEVNQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            2113..3045
                     /gene="flgL"
                     /locus_tag="KR440_002562"
     CDS             2113..3045
                     /gene="flgL"
                     /locus_tag="KR440_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBH0699600.1"
                     /translation="MRVSTGMMSSNYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            3061..3453
                     /locus_tag="KR440_002563"
     CDS             3061..3453
                     /locus_tag="KR440_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HBH0699601.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDSLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDNGC"
     gene            3447..3659
                     /gene="csrA"
                     /locus_tag="KR440_002564"
     CDS             3447..3659
                     /gene="csrA"
                     /locus_tag="KR440_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBH0699602.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            3681..4076
                     /gene="fliS"
                     /locus_tag="KR440_002565"
     CDS             3681..4076
                     /gene="fliS"
                     /locus_tag="KR440_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBH0699603.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIARAAILD
                     KNTQKAHRELVRVQDIFTELMVTLDQNAGQWAKDMYKVYDFVRYELSRANIRKDIQVI
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            4076..4432
                     /gene="fliS"
                     /locus_tag="KR440_002566"
     CDS             4076..4432
                     /gene="fliS"
                     /locus_tag="KR440_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBH0699604.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELIRVQNIFTELMTTLDQDAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            4453..5976
                     /gene="fliD"
                     /locus_tag="KR440_002567"
     CDS             4453..5976
                     /gene="fliD"
                     /locus_tag="KR440_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBH0699605.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSVNSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVRQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLKVIGNDNLAQQIANDNGIT
                     FANDASGNKVASVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            5979..6314
                     /gene="fliT"
                     /locus_tag="KR440_002568"
     CDS             5979..6314
                     /gene="fliT"
                     /locus_tag="KR440_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HBH0699606.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISSKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            6416..7288
                     /locus_tag="KR440_002569"
     CDS             6416..7288
                     /locus_tag="KR440_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HBH0699607.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTTN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTDAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            7381..>9263
                     /locus_tag="KR440_002570"
     CDS             7381..>9263
                     /locus_tag="KR440_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBH0699608.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRSSIESELIIVDTGSTDD
                     TINISRKYTENVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNIDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFEELKENDNSPYTNYQLGKNFIILGKYHEAL
                     YYLEKSYELYSKLESAPGYLITSLVKTYLYLGKHKKCEKLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYESVISEYNNIEDMEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     YKNIEEVINNIKDDEKEHVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEII
                     YAYIIKVAFEDKIDLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTSDI
                     KKIRVYKAISKVVLESISLSDEKYKEIFYLYIMYSYKYIRYIYRNLSDFELIKFVCND
                     LDKFTIEFKHLLDIKNKVNDANEKLEYIHSMRKLLNEYPFYNKIIKILISELEENIKE
                     SEE"
BASE COUNT         3955 a          956 c         1632 g         2720 t
ORIGIN      
        1 aattatataa atgtttttat tttatgagcg ataattaaat tataagtatg taattttgga
       61 ggtgaaacta tggatattaa aaatgtaagt tcaaatattg tagtgaataa ttatttaaag
      121 aatacatctg gtgtaaaaaa agtggaagaa gttaaaaaaa ttaataaaac agacaagata
      181 gagctatcta aatttgcaaa ggaaatggca agtgtgtcaa atatgagtga tgagaatatt
      241 aggaataaaa aagtcgatga aataaaaaat gcattagaaa atggaactta caaaataaaa
      301 ccaaaagaaa ttgcgaaaaa aatgatagag gatatgcgag gataaagtca tgattacaga
      361 cttaaaagtt ataatatatg aagaaaaaaa tataattagt aagatgatgg aattattaaa
      421 agaacaattt gattatatag ttgaaaaaga cttagaaaat ttaaatatgt taaatcctaa
      481 actagaagat attagcaggg agcttgcatc catagaaata aagagaagac aactttttgg
      541 tgatgatatt tcaatgagtg aagttgttga aaatagtaat gatagatatt taaaagaaat
      601 atatacagac ttgaaacaca ttcttgcttt agctaaaaat caacaagaat caaatgattc
      661 tctaataaag aaagaactta tttttacgaa aaaaatgatt aatttcataa aaccagctga
      721 taagcaaact gcaacttata attcttatgg aaatattaga aaataaatat taggaggaga
      781 aatatggcag gattattttc cattttaaat acagctaatt caggaatgaa tgcgcaccaa
      841 aaagcaatac agacaatttc tcataatata agtaatctag atacagacgg ttattctagg
      901 caaaggactg agtttgctac aaatagtcca atgtatatgc ctagtttgag taattcaatt
      961 gcaagaggac aattagggac tggagtacac gtaacggatg taacaagagc tagaaatagt
     1021 ttttatgatt accaatttag agcagaagct cataagtatg gtaatacagt ttcaaagtat
     1081 gactattata atactataga aactataata aatgaaccat cagactatgg aatatctgca
     1141 ggtatagacg acttttttaa aggatgggaa tcattagcaa aggatataaa tagtgatagt
     1201 aaaaaaagtc tagtggtaga aaatgcatca aatctagcaa ctttaataag tgaaagttat
     1261 aagaagctta ataagttaaa gggagatata gacgataata taaactctga aataaaacag
     1321 ataaatgaca tgttaaaaag tcttgaagat ttgaataaat ctattgatgt tatatctggt
     1381 tctggctcta ctccaaatga tttacttgat gaaagagata gaatattaga tgacttaagc
     1441 tttaaattag accttgaaaa tagcgatgtt aaaaatatgc tttcagatgg taaattagaa
     1501 cttaatgaat taaaaaatgc agatggaact tggaaaacag gtgtatcagg aaccttacaa
     1561 ggcttgtttg aaatgcatgg taagattgat acatataaaa gcgatttaaa agatgtatct
     1621 gatgggttag ctaagcagat aaatgatgtt tataatacat ctgctggaat aacagtaaca
     1681 gattttttta taacttcaaa tgtagatggt gaagatatca taaaggtaaa tccagctata
     1741 aagagtaacc caaatgaatt aaaacttact gctgatgagg cttcaaagat tgctaagtta
     1801 aaggatgaaa aaatagatat tggggtggct ggtggaaaag taagtacaat aagtgaccac
     1861 tataaagcct ttgcagaatc agtaggttta gattctcaaa aagtgaatca agatgaagtg
     1921 aatcaacgta aaattataaa taatattgat aactcacgaa tgagtgtttc aggcgtatct
     1981 ttggatgaag aaatgacaga gcttataaaa atacaacgct cctatcaagc tagtgcaaaa
     2041 gttatgtcaa cagctgtgca actgttggat gttgtaataa atggaataat ttaatagaag
     2101 ggggaattta taatgagagt tagtacaggt atgatgtcta gcaactatct aaactcacta
     2161 caagataatc tacaaagatt agataaggtt aatagacaag ttaatacaac gaaagaaata
     2221 aacaagctat cagataatcc atataaggca ataaaaatat tgaactcaaa aagtgaaata
     2281 aaaactatgg agacttatgt tgagaattgt aaagatacag ggaattggtt agaaactact
     2341 gatactgcat tagatcagct tggaaaatta ttatcggaca taaagaaaac tttggtggca
     2401 agtggtgatg gtgcatattc agatgatgag ctaaagactc ttagtaatga aactaatgaa
     2461 aagatgaaag aaattgcaaa tgccttaaat gcaacacatg aaggaaagta catattttca
     2521 ggttcaaata caggtgtacc acctgttgaa tgtatagata atgctgacgg ttcagtgagc
     2581 ttaaagttta attctagttt aaatcttaat aaattaaatg actctttgag tatggatata
     2641 gctcaaggaa taagtgtaga ttataatgtt aaattgagta gccttggatt tgacccaaca
     2701 aagactgacc catttgaaga gttaaataat atatcaaaga aattagcaaa tccagatgct
     2761 gatgctataa aagagctgac aacaacttgt ttaggcgaca tggaacaatt aatagaaaat
     2821 acagtaaatg ttaggtctat atatgggaca aaagcaaata cagtagaggc tatgaaggaa
     2881 aaaaatgatg atagtttgat acagttaaag gatattttgt cacaaaatga agaaatagat
     2941 tatggagaaa aactcgttca attaaaggca gcagagttaa cttatcaagc ttcactacaa
     3001 acaggaggaa agttatttaa tgtatctata ttagattaca tatagtaaaa taacgcagat
     3061 atgatgaagg ttacattaaa aaaaggtata ttaggttttg aaaatttaaa agaatatgag
     3121 cttttagata tagaagataa tgattcttta aaagaattta attctactga agaagaaggt
     3181 ataggatttg ttgttgtatc tccatttgag attatcaatg aatatgaaat agttttaaat
     3241 caggaaacaa tagaaaaact agaagtgaaa tctccaaagg atgttgtact atttaatata
     3301 attactcttg gtcagacatt tgaagaatca actgtgaaca tgaaagctcc tatagtgata
     3361 aatataaaaa ctaattttgg aatgcaaata attttacagg atgataaata ttcaataagg
     3421 catccattat tgagaggtga taatggatgc tagtaatttc aagaaaaaaa gatgaagcag
     3481 tacttatagg agaaagcata gaagtaaaaa ttgtaggggt agatggaaac aatgtgaaac
     3541 tagctatatc ggcgcctaac aatataagta tattaagaaa agaaatatac gaaaaagtaa
     3601 agagtgagaa tataaaagct acaaataaaa atataaaaat tttaaagtca ttaaaataat
     3661 aagcttggag gaagtaatat atgtatggag aaaatcctta taattcttat aaacaaaatg
     3721 ctgtttttat ggcatcaaag gaacaattgt tacttatgct tgttgatgga gcagtaaaat
     3781 ataccaaaat agccagagca gctatcttag ataaaaatac acagaaagcg catagagaac
     3841 ttgtaagagt tcaggatata tttacagaac taatggttac attagaccaa aatgcaggac
     3901 aatgggcaaa ggatatgtac aaagtttatg attttgtaag atatgaactt tcaagagcca
     3961 atattagaaa agacatccag gtaatagata atgtgttgcc tgtaatagaa caaattaaag
     4021 acacatggca tgaagctgat aaaaaaagta aagaagaaag aagtaggtac aaataatgga
     4081 attaaattta gctgaattag ataagttatc aaaagaggaa ttactattga tgcttgtaga
     4141 tggcacagta aaatatacca atatatccaa agaagctctt ttaaataacg actatttgaa
     4201 agcacataat gaacttataa gagttcaaaa tatatttaca gaactaatga ctacattaga
     4261 ccaagatgca ggtcaatggg caaaggatat gtataaagtt tatgaattta taaaatctga
     4321 attagcaata gctgatgaaa ataaagatat taaaataatt gatgatatat tgccaatagt
     4381 aaaacaaatt agagatacat ggcatgaagt ttacaatcaa ctaaaaatat aattagatgg
     4441 agggataaag gtatgtcaag tataagtcct ataagagtta caggtctttc aggaaatttt
     4501 gatatggaag gcataatcga agctagtatg attagagaca aggaaaaagt tgataaagca
     4561 aaacaagaac aacaaatcgt taaatggaag caagaaatat atagaaatgt tatacaagaa
     4621 tcaaaagatc tttatgataa atatctaagc gtaaattctc ctaatagtat agtaagtgaa
     4681 aaagcatact cttctacaag aataaccagt tctgatgaaa gtattatagt agcaaaaggc
     4741 tcagctggtg cagaaaaaat aaattatcaa tttgcagttt ctcaaatggc tgaaccagca
     4801 aaatttacta ttaaattaaa ttcaagtgaa cctattgttc gacagttccc tccaaatgcc
     4861 agtggagcta gttctttaac tataggagat gtaaatatac caatatctga acaagatact
     4921 acaagtacta ttgtaagtaa gataaactcc ctttgcgcag ataatgatat aaaggcttct
     4981 tatagtgaga tgacaggtga attgattatt tcgagaaaac aaactggttc gtcatcagac
     5041 attaatttaa aagtaattgg aaatgacaat ttagctcagc aaattgctaa tgataatggt
     5101 atcacatttg caaatgatgc tagtggaaac aaagtggcaa gtgtatatgg aaaaaatcta
     5161 gaagctgatg taactgatga acatggaaga gtaactcata taagtaaaga acaaaattca
     5221 tttaatatag ataatattga ctataatgta aattcaaaag gaactgcaaa gttgacttct
     5281 gtcactgata ctgaagaagc tgttaaaaat atgcaagcat ttgtggatga ttataataaa
     5341 ctgatggaca aggtctatgg tttagttact actaaaaaac caaaagatta tccgcctctt
     5401 acagatgccc aaaaagaaga tatgacaact gaagaaatag aaaaatggga aaagaaagct
     5461 aaagaaggta tacttagaaa tgatgatgag ttaagaggtt ttgttgaaga tattcagtct
     5521 gcattttttg gagatggaaa aaatattatt gcattaagaa aactaggtat caatgaaagc
     5581 gaaaattaca ataaaaaagg tcaaatatca tttaatgcag atactttttc aaaggctctt
     5641 atagatgata gtgataaggt atacaaaaca ctagcaggtt attcttcgaa ttatgatgat
     5701 aagggaatgt ttgaaaagct aaaagatatt gtatatgaat attctggaag ttcaacttct
     5761 aaacttccta aaaaagcagg tatagaaaaa actgcttctg ctagtgaaaa tgtatattca
     5821 aaacaaattg cagagcaaga aagaaatata agcaggttag ttgaaaaaat gaatgataaa
     5881 gagaaaagac tttatgctaa atattcagcc ttagaatctt tgttgaatca gtattcttcc
     5941 caaatgaatt atttctcaca agcacagggt aattaattat ggataaatta aaagaaaaat
     6001 taaatttata taaagatata tctttacaaa ttataaattt gattgaaaag gaagaatata
     6061 taaagataag tagtaagtta ggtgaaagac aagagattat aaatagtgta agtgaaattg
     6121 atagaaatga ttttatacag ctttataata gaatggaatt aattgaaatt gatagtagga
     6181 taagagatat tttacaagga caattattag aagtaaaaaa agaattacat gagtataagc
     6241 ttactaaaca agtgaacaca atgtattata acttaaatag agaaaaagta aatattttta
     6301 ataaaaaagt ataaagttat gtaccatctt gtccgattat ataaatgaag aattaataaa
     6361 aaagattaaa agtagaaatg acaaggatgt caactatact aaggagggta aaataatgag
     6421 agttaataca aatgtaagtg ctttgatagc aaataaccaa atgggaagaa acgtaaatgc
     6481 acaaagtaag tctatggaga agttatcttc tggtgttaga attaagagag ctgctgatga
     6541 tgctgctggg cttgctatat ctgagaaaat gagagctcaa ataaaaggtt tagaccaagc
     6601 aggaagaaac gttcaagatg gtatatctgt tgtacaaaca gcagaaggtg cattagaaga
     6661 aactggtaat atactacaaa gaatgagaac tttatcagta caatcttcaa atgaaactaa
     6721 tactgctgaa gaaagacaaa agatagctga tgagttgtta caattaaagg atgaagttga
     6781 aagaatatca agctcaattg agtttaatgg taagaaatta cttgatggaa gttctacaga
     6841 aataagatta caagttggag ctaattttgg aacaaatgtt gcaggaacaa ctaacaacaa
     6901 caatgaaata aaagttgctc tagtaaatac ttcaagtata atgagtaaag caggtataac
     6961 aagttctaca atagcaagct taaatgcaga tggaacttct ggaacagatg cagctaaaca
     7021 aatggtgtct agcttagatg tagctcttaa agaattaaat acatcaagag caaaattagg
     7081 ggcacaacaa aatagactag aatcaactca aaacaactta aataatacta tagaaaatgt
     7141 tacagcagct gaatcaagaa taagagatac agatgttgct tcagaaatgg ttaacttatc
     7201 taaaatgaat atattagttc aagcatcaca gtcaatgctt gctcaagcta atcagcaacc
     7261 acaaggagtt ttacaattat taggataaaa taattttgta tattggatat agaagaaagg
     7321 gtaaggcttt gctttactcc tttctttttt tacaaatata cttttgaaag gaaaatatat
     7381 atgttactta gtatagttat gattgttaaa aatgaagaaa aaatacttga aaaaacttta
     7441 aaatcattaa ccacattaag aagttctata gagagtgaac ttataatagt tgatacaggt
     7501 tctacagatg atactataaa tatatctagg aaatacacgg aaaatgtgta ctttcataat
     7561 tggaataatg atttttctag tatgagaaat atatcaatat cctatgctaa aggtgaatgg
     7621 ttacttatat tagatgcaga tgaaatacta attgattctt caactattgt gaaatttttt
     7681 aaagatggtc ttgataaaaa atttaattcg gcctcaatac aattaaaaaa tctttactca
     7741 tatgataaaa aattatatgg atattgctca gtgcttagac tttttaaaaa catcgatttt
     7801 agataccatg gtaaagtaca tgaacaacca atatacaaaa accctatatt taataatgtt
     7861 gctgattttg aacattatgg atatttgttt gaagatgaag aaataagatt aaataaagta
     7921 aaaagaaatg aaaaactgtt atttgaagaa ttaaaagaga atgataattc tccatataca
     7981 aattatcaat tagggaaaaa ttttattata ttaggaaaat atcatgaggc tttatactat
     8041 cttgagaaat cttatgaatt atattctaaa ctagaatcgg cacctggtta cttgataaca
     8101 agtcttgtaa aaacatattt atatttagga aagcataaga agtgtgaaaa attgtgtcta
     8161 aagtatatta agaaagacat gaataatatt gatatatatt attatattgc acaagcacaa
     8221 gtagatttag ggaagtatga aaatagtata gattcgtata agagatatat ttatttgtta
     8281 gataattatg aaataagtac acaagcaaat agtttatttt cagacactga tactgtaggg
     8341 cttagggatg aagctattat tacactgata aagatttatt ataaacttga aaaatatgag
     8401 tctgtaattt cagagtacaa taacattgag gatatggaaa agaagaagaa tgtgtacttt
     8461 agcttattta tgtccctata caaattgaac agatttgaag atataaaaaa ctattataaa
     8521 gaagtttcaa attctaaaat tgaaaaaaat agtttttata aaaatataga agaagttata
     8581 aataatatta aagatgatga aaaagagcat gtatataaaa tcctttgtga cattgaaggt
     8641 aattatggca ttttaaatca gataaggata aataaatgtg tatctataga taagtgtaaa
     8701 gaaatactca atgaagaaaa agaaataatt tatgcatata ttataaaagt agcttttgaa
     8761 gataagattg atttactaga tattttttat gaaatggaat atgtatggat agagaaatac
     8821 ctaaaatatc ttttagcact agaaagaagt tttaatttag tactatataa gtacataatt
     8881 agtcgtccga atacatcaga tataaaaaag ataagagtat ataaagctat atctaaagta
     8941 gttttagaaa gtataagttt aagtgatgaa aaatataagg agatttttta tctttatata
     9001 atgtattctt ataagtacat aagatacata tatagaaatt taagcgattt tgaattaata
     9061 aaatttgttt gcaatgattt agataagttt actatagaat ttaagcatct acttgacatt
     9121 aaaaataaag taaatgatgc taatgaaaaa ttagagtata tacatagtat gaggaaatta
     9181 ctaaatgagt atccttttta taataaaata ataaaaatac ttataagtga attagaagaa
     9241 aatataaaag aaagtgaaga att
//
LOCUS       DAEPOZ010000145         9161 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.145, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000145 DAEPOZ010000000
VERSION     DAEPOZ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9161
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.145"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            250..816
                     /locus_tag="KR440_002571"
     CDS             250..816
                     /locus_tag="KR440_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HBH0699609.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
     gene            1121..1582
                     /locus_tag="KR440_002572"
     CDS             1121..1582
                     /locus_tag="KR440_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0699610.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            1650..3896
                     /locus_tag="KR440_002573"
     CDS             1650..3896
                     /locus_tag="KR440_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0699611.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            3889..5721
                     /locus_tag="KR440_002574"
     CDS             3889..5721
                     /locus_tag="KR440_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0699612.1"
                     /translation="MSRMGRGPMGKSMEAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            6440..7717
                     /gene="hflX"
                     /locus_tag="KR440_002575"
     CDS             6440..7717
                     /gene="hflX"
                     /locus_tag="KR440_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBH0699613.1"
                     /translation="MDMTKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNRELYDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVL
                     DRILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDCVRCKFLIPYDKGHVVSYFNEN
                     TSIINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            7950..8447
                     /locus_tag="KR440_002576"
     CDS             7950..8447
                     /locus_tag="KR440_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0699614.1"
                     /translation="MDAESLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIQIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEVEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            8497..9021
                     /locus_tag="KR440_002577"
     CDS             8497..9021
                     /locus_tag="KR440_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBH0699615.1"
                     /translation="MKSVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKDLQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKVPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILNYIRKL"
BASE COUNT         3656 a          908 c         1636 g         2961 t
ORIGIN      
        1 aagtaaggca tttagaataa aattttaaaa aaattataat taaaaaaata atgaaaatat
       61 ataatattag tttagattat aaaaaataaa tatacaaata cttataaata caagttatat
      121 acaagattta tagtagagta ataatcttat aaaacaattt aataaatata aattaaatta
      181 tatgtgtata tattgtactt taatagtgat ataataataa aaaaattttt aaagagggtg
      241 atattacata tgagaaaact tgaagagata ttagaatata ataaatcatt tgtagagaac
      301 aaagaatatg aacaatatgt gacatcaaaa catccaaata aaaaaatagt agtcttatct
      361 tgtatggata caagattaac tgagcttttg cctaaggcta tgaatttgaa aaatggagat
      421 gtgaaactaa taaaaaatgc aggagctact ataatgcatc cttttggaag tattatgaga
      481 agtgttttag ttgcaattta cgaatttgat gtagatgagg ttatggttgt agggcatcat
      541 ggatgtggta tgtgtaatgt tgatacagat aagttacttg gtaaaatttt agatagagga
      601 atatctaatg atgtaatact tacacttcgt agtgctggaa ttgatacaaa acaatggtta
      661 catggattcg attctgcaga agaatctgta aaagatagta ttgatttaat aaaagggcat
      721 cctttaattc cagatggtat tattgtacat ggtctaatta tatcacctga aactgggaag
      781 ttagacgttg ttgtaaatgg atatgaagat aagtagagta ttgtaatgtt atataatcaa
      841 gatactgttg aatatgatgt tgtttcaaaa tttattttgg gacaactttt ttatttgaaa
      901 atatatgaaa aggtttattt taagctttac aataaaaaat ataagtagct gactaattaa
      961 aaatcttact attatttatt aaaattataa ctatatgata taaaaatatt taataaatga
     1021 aatttatatg aattttttat aaatatattg aaaaaatgca tgcagtatag taaaataatg
     1081 cttagccgac taagtaatat aaaaaataag gagggcaagt atgaatagca attctgaaga
     1141 aaaattaaat gatgttttct tgcaattaat gcgaatatat tttataagaa ctcattttta
     1201 ttttgataaa ttaggcttat atcatggtca accccaaata cttttttctt taaagatgaa
     1261 agatgggcaa agtcaaaaag aattagcaga taaaaggaaa gtaaaagcat ctacaactac
     1321 agttatgata aagaggatgg aaaaagctgg acttgtggaa agaaaacaag atgaaaagga
     1381 ccaaagagta tctaggattt ttttgacaga caaaggttta gaaatatgta ataaagttga
     1441 agaatttaac aaggaattgg aaagagagtt ttttttagga tttagtgaag aagaaaaaat
     1501 agtacttaga agacttctaa tgcaagtacg agataattta aaagcaggat gtcataagca
     1561 tgaagatgaa aaatattttt aaagtttaaa ataatgtttt tcaatttaat gttattagac
     1621 taattaatat tacagaaggg aagaaatgta tgtttaaatt aattaaatat ttaaagaaat
     1681 cggcattatc aatagtaata atagtctgtc tattggtaat tcaagcggta tgtgaccttt
     1741 ctttaccaga atacacatcc aatatagtaa atgtaggtat acaacaaggt ggagtagaaa
     1801 actctgttcc atctgtgatt agagagagtg aattaaataa aattacttta tttatggata
     1861 aaagtagtaa ggataaagta ctagataatt atactttgct gaataaaaag gattatgtta
     1921 agtataaaga taagtaccca ggtctaaaag atgaatctct ttatgagtta aatactaaag
     1981 acaaagacac tatagatgat ttaaatgtta tttttggtaa agctatttta atagtatcag
     2041 ggcttgaggg cgatagtaaa gaggtaaaag ctatgaaagc tcagttaatg agtaaacttc
     2101 caccacaagc tactcaaagt ggagatgttg atatatttaa attgttatca gcaatgccaa
     2161 aggaacagtt agatacattg actaaagaga tgagcaaagg ctttgaaagt atgccagaga
     2221 gtatgataac acaaagctct gtttcatatg taaggtcaga atatgagaaa ataggtattg
     2281 atacagaaaa aactcaaaat aattatatat tatttactgg tgctaaaatg cttggtatag
     2341 caatgattag tatggtagct acaataacag taggattttt agcagcaaga gtagcagcaa
     2401 gtgtaggtag aaatttaaga agtggagtat ttagaaaagt tatgtcattt tcaaatactg
     2461 aaatgaatga attttctaca gcttcattaa ttactcgtag tacaaatgat attcaacaga
     2521 tacaaatgtt gatggttatg ttgcttagaa tagtattcta tgcacctata atggctatag
     2581 gtggagtgat aaaggtttta aacacaaata cttcaatggc ctggattata gcagtagcag
     2641 tagttgctat cttatcatta attgtagtgt tattctcagt ggttatgcct aagtttaaac
     2701 tggttcaaaa gcttgttgat aagttaaact tagtaagccg tgaaataatt actggtattc
     2761 cagttatcag agcttttagt actgaaaaac atgaagaaga aagatttgat aaagcaaata
     2821 aaaatttaac taaaacaaat ctttttgtaa atagagttat gacatgtatg atgcctgcta
     2881 tgatgcttat aatgaacctt atcacagttc ttatagtttg gagaggtgca tacagtgttg
     2941 attctggagc aatgcaagtt ggagatatga tggcatttat acaatataca atgcaaataa
     3001 taatgtcatt cttaatgata tcaatggtat ctattatgct tccaagagca tccgtttcag
     3061 ctacacgtat agatgaagta ttagtgtcag atataagcat aaaagaccca gaaaaatcag
     3121 aggcacaaga gtttaaggaa gataaaaagg gtcttgttga atttaaaaat gtttcgttca
     3181 tgtatccaga cgcagaagaa cctatattaa caaatatatc ttttacagca aagccaggag
     3241 aaacaactgc ctttatagga agtactggta gtggtaagtc aactcttata aatttaattc
     3301 ctcgtttctt tgatgttact gaaggagaaa tattacttgg tggagttaat ataaaaaatg
     3361 tttctcaaca tgatttaagg gaaaaaattg gatatgtacc tcaaaaggga gtattgtttt
     3421 ctggtactat agaatccaac ttaaaatatg gtagaaaaga cgcaacagat gaagaaatga
     3481 gaacagcagc tgaaatagct caagctacag aatttataga ttctaaacca gaagctttta
     3541 aaactgaaat ttctcaaggt ggaacaaatg tatctggagg tcaaaaacaa agactttcta
     3601 ttgcaagagc aatagcaaaa aatccagaag tttatatatt tgatgatagt ttttcagcac
     3661 ttgacttaaa aactgatgca gctcttcgta aggctttaaa atcaaaaact gctgaaagta
     3721 cagttctaat agttgcacaa agaattagta ctatcttaaa tgcagaacag ataattgttt
     3781 taaatgaggg agaaatagtt ggaataggaa cacataaaga gcttctaaaa aattgcgaag
     3841 tttatgagca aatagcttta tcacaacttt caaaggagga gctagctaat gagtagaatg
     3901 ggaagaggtc ctatgggaaa atctatggaa gcaggacaaa aagcaaatga ctttaaaggg
     3961 actatgcgaa aactaattgc atatttatct aaatttaaaa tatcaataat tttagtaatt
     4021 gtatttgcta ttggtagtgc atcgttttct atagtaggtc caaaaatttt aggaaaggca
     4081 actacaaaga tatttgaagg gttagtgagt aaagtttctg gaggaaacgt aggtatagat
     4141 tttaatgcaa ttggaaaaat cttaaccttt ttacttttcc tttatttgat aagtgcactg
     4201 ttctctttta tacaaggatt tataatgagt ggaatttctc aaaaagtatc ctataattta
     4261 agaaaagaga tttcagcaaa attggataga ttaccaatga agtattttga tactaagacg
     4321 catggtgaaa tattatcgag aattacgaat gatatagata ctttaaatca aagtttaaat
     4381 caaagtatga cacagcttat aacatcagta acaactatga taggtgtttt gataatgatg
     4441 ttatctataa gtggaattat gacattagtg gcagttttaa tattaccaat atcaatgttt
     4501 gtaatttcaa gaataataaa gaaatcacaa aaatacttta gatatcaaca agaatattta
     4561 ggaaatgtaa atggtcaagt tgaggaaact tatagtggcc aaacaatagt gaaagcattt
     4621 aatcgtgaag atgaagtaat tgaagagttt gataaactaa atgatagcct gtataattca
     4681 gcgtggaaat ctcaattttt atcaggtata atgcaaccac ttatgatgtt tattggaaac
     4741 ttgggatatg taatggtatc aatcttagga ggttggcttg caattaagaa aacaattgag
     4801 gttggtgaca tacagtcatt tatacaatat gtaagaaact ttacacagcc aatgactcag
     4861 attgcacagg ttgccaattt attgcaatca acagcagcag cttctgaaag agtatttgag
     4921 tttttagaag aggaagaaga agtccaaatt gttgaaaatg cagtttcaat agatggttta
     4981 gaaggtaaaa ttgattttga aaatgtcaac tttggatata acccaaacaa gactataata
     5041 aatgatttct cagtaaatgt taaacctggt caaaaagttg ctattgttgg acctacagga
     5101 gcaggtaaaa ctacaatagt taaactgtta atgagatttt atgatgttaa tagcggttca
     5161 atactgattg atggtcataa tattaaagat tttaatcgta gtgagcttag agaaatgttt
     5221 ggtatggttc ttcaagatac atggttattt agtggttcaa ttatggaaaa tataagatat
     5281 ggtaagttga atgctacaga tgaagaagtt atagaggctg ctaaatctgc acatgttcat
     5341 agatttatta agacgttacc agatggatat aagatgaaat taaatgaaga agcaagtaat
     5401 gtttctcaag gtcaaaaaca attattgact atagctcgtg ctattttagc tgatcctaaa
     5461 attttaatat tagatgaagc tacaagttct gtcgatacta gaacagaagt attgattcaa
     5521 aaagctatgg ataatcttat ggaaggtaga acaagttttg ttatagccca cagattatca
     5581 actatacgtg atgctgatat gattcttgta atgaatgaag gagatatagt tgaacaagga
     5641 aatcatgagg aacttctaaa gaaaggcgga ttctatgcca atctttacaa ttctcaattt
     5701 gaagaagatg aagcaatgta acaaaaaact atcttagcat agacatgtaa ttaataagat
     5761 aaaataaatg agggtgcctt aaaatgaact ttttaattca taaggcactc ttttgtgtat
     5821 gaaataaagt gtatttttat cattttaaca atgaaaaaga ggattatcta tattttgaga
     5881 tagccacata attttatgtc tttacaaagt gtttatttta ttataatatg tagtaaggaa
     5941 aatgttggag gtgattatca aaatgtatat ttaattttat tctttaaatt ttattataaa
     6001 tagaatttaa ttttaatagt tgatgattat attgaagtat atgtttttat tacttttatt
     6061 aatcatgcaa ataactactt aaatttaaga atagggttaa atataaaatg ataattacat
     6121 ataagttaat taaactaatg tatttttaat atatatttag aattttaatc cttcttatga
     6181 tataagtagc ttgttttgta ttacttagat ttgtaaggag tttttttatt gaaaaaactg
     6241 cataatatat ttgggaggag gtgtaagaaa gtgatttttt atataagatt gagaaaataa
     6301 tgcacgatta tggttattat aatagtaaat aagaagtgtc atttaaggat aagataaaaa
     6361 gcaaaattct tgatgaattt aattatagag ccataatctc gtgctataga ttaaataaaa
     6421 aatagcagat aaggagaata tggatatgac gaaaaaagga ataacagtag gattaaacat
     6481 aaataataaa tctgaggact ttaatgaact aatgattgaa cttgaaaact tatgttcagc
     6541 atgtgatatt gatgttgtag ggagtataac tcaaaatgca aagcaggtta atagagcatt
     6601 ttacattggg acaggtaaag ttgaggaaat attaaactta ataaagaaag aaaacataga
     6661 gatagttata ttttacaatg aactttctac atcacaacta aaaaatcttg aagaaaagct
     6721 taattgtgaa attatagata gaacggcatt gatacttgat atatttgcac aaagggcaaa
     6781 aactagagaa gctaaacttc aagtagaagt agcaagttta aaatatatgt taccaagact
     6841 tataggttca aatgaaaatt taggacgtca aagtggtgga gttggtacta aaaatagagg
     6901 ttctggtgag aaaaaattag aacttgaccg tagaagaata gaagaaaaga taacatcttt
     6961 aaatagggag ttgtatgatt taaaattcca aagagaaact caaagaagta tgagaagaaa
     7021 atcaaatctt ccaaatgtag ctctagtcgg gtatacaaat gctggtaaat cttcaataat
     7081 gaataaacta gtagatatat ttaaaaactc tgaagaaaag aaagtgtttg agaaaaacat
     7141 gttatttgct acacttgaaa cttctgtcag aaatatagtt cttgcaaata acaaagaatt
     7201 tttattatct gatactgttg gttttgtaag taatctacca catgacttag taaaagcatt
     7261 taggtcaaca ttagaagaag catgtgaggc agacgtactg ttacatgtta ttgatatttc
     7321 aaatccaagc tataaaagtc atattaaagt gacagaagat actttaaaac aaataggtgc
     7381 agatggtata cctatgatac atgtgtataa taaaattgat ttaatagatg tagaagtatt
     7441 ggatagaatt ttagatagta tcgataaaga aggtatattt gtttcagtta aaaaagatat
     7501 aaatatagat aaaatgataa aatgtatttg tgatagcata tttaaagatt gtgtaagatg
     7561 taagttctta attccatatg ataaaggtca tgtagtttct tattttaacg aaaatacgag
     7621 cattataaat acagaataca gagaagatgg agctatttta gatgtagaat gtagcaatat
     7681 agaatataat aagtataaaa agtatgcttt agagtaaatt ttgaaaaact tattatagcc
     7741 taaaatatat ttaatttata atttattata aattgattgt aaatatgatt ttatgaaacg
     7801 gtatagccat aatgctatgc cgttttgcca tttataataa aaataaattt attgacacaa
     7861 tagtaactaa ttactataat ataaagtaac tagttactat ttaaaattat caaaaaatta
     7921 aaatctaaga taaagagaag tgtataataa tggatgcaga aagtttaatg aaaaaatata
     7981 tggtagatga tgatagtaga aaacaagcta tatttgcaag tatatttgta atgcaaaata
     8041 aacttcaaac tacatgtgat aaattagacc cagaattaac aatgaaacaa tggttacttt
     8101 tagctattgc ggactctgta gatgaaactc ttacattaac caaacttgga gaattaatgg
     8161 ggtgctcaag acaaaatgta aaaaaactag ctattgcttt agaaaagaaa ggttttatac
     8221 aaattgagca aaatgaaaaa gattcaagag ctatctgtct tgtgacaaaa gatagagtgc
     8281 aagagtattc taaaaaagta agaggtatgc aggaaaaagt attacagctt ttatttgaag
     8341 attttacaga gaaagaagtg gaacagttat tttttggaat tatgaaatta agtataggaa
     8401 ttgaaaatgt gaaaaattat gtagaaaata atgacgttgg agaataagca agtatcaaat
     8461 caactttgta tgacatcaga gaggggggta atttatatga aaagtgtagt agtgatttat
     8521 aagagtaagt atggttcaac aaaaaaatat gctaaatggc tttcagaatt attgtcttgt
     8581 gatatttttg aaactaagaa tattaatata gaaaaggtta tgaagtatga tacgattatt
     8641 ttaggaggag gtctttatgc ttgtaatata gcgggaattt ctttcttaaa aaaaaattat
     8701 aaagatttgc agaaaaaaaa tttagtagta tttggcgtag gagctgctcc atatgatgat
     8761 gaagtaataa agaaattaag agaagtaaac tttaaggatg aattaaacaa agttccttgc
     8821 ttttattgtc gtggggcatg ggatgagaca aaaatgtctt ttatagatta tactctttgt
     8881 agttttttta aaaaatctat agctaaaaaa gaacgtcaag gtcttaaacc agcagaagca
     8941 cctttattgt ctggagaagc ctttgattgg tgtaataaag aaaatttaac tccaatactt
     9001 aattatataa gaaaacttta agaggtatat atttataatt atttattatc taaagttaga
     9061 tttcatagaa acttactaga cattatttat aaaataatat tattaaagat ggataattta
     9121 attgtttcaa taaggttata attttaaaaa atgtttattt t
//
LOCUS       DAEPOZ010000146         9090 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.146, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000146 DAEPOZ010000000
VERSION     DAEPOZ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9090
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.146"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            25..210
                     /locus_tag="KR440_002578"
     CDS             25..210
                     /locus_tag="KR440_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBH0699616.1"
                     /translation="MFFISDIYTKSPIKFDTPLQKEVYKILQKLDIDFERVDTDEAIT
                     MEDCVQINKKLNMKMVI"
     gene            complement(275..3049)
                     /locus_tag="KR440_002579"
     CDS             complement(275..3049)
                     /locus_tag="KR440_002579"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBH0699617.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAQALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGVIATLLCMFMIIQM
                     QRKGMLILDTSSILSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRSMNSNSKN"
     gene            complement(3221..4477)
                     /locus_tag="KR440_002580"
     CDS             complement(3221..4477)
                     /locus_tag="KR440_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBH0699618.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            complement(4549..6051)
                     /locus_tag="KR440_002581"
     CDS             complement(4549..6051)
                     /locus_tag="KR440_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBH0699619.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            complement(6142..7332)
                     /locus_tag="KR440_002582"
     CDS             complement(6142..7332)
                     /locus_tag="KR440_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0699620.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIEYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYTIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
     gene            complement(7571..8659)
                     /locus_tag="KR440_002583"
     CDS             complement(7571..8659)
                     /locus_tag="KR440_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699621.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
     gene            complement(8944..>9090)
                     /locus_tag="KR440_002584"
     CDS             complement(8944..>9090)
                     /locus_tag="KR440_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0699622.1"
                     /translation="SIELLENTTKSINEIAEIVGYNSLNNFYKNFKIEKGMTPKVYKL
                     NTKN"
BASE COUNT         2923 a         1692 c         1067 g         3408 t
ORIGIN      
        1 aaacggaaag gaatgaaata aatcatgttt tttatcagtg atatttacac aaaatcacct
       61 attaaatttg atactccatt gcaaaaagag gtatacaaaa tattacaaaa gttagatatt
      121 gactttgaac gtgttgacac agatgaagca attacaatgg aagattgtgt tcagattaat
      181 aaaaaattaa atatgaaaat ggtaatataa aacaacatta aacaaacaaa aaccgcaaag
      241 ttacaatata tagtactttg cggtttcata agaattaatt tttactatta gagttcatac
      301 ttctactatt tttcattgtt ctaagtatta gctttacaaa ttcatttgac aataatatag
      361 tggatgcaaa tagtattatt tttatccaca tatcaaagtc caaagaaaca gaattaaaga
      421 aactctggaa cacttgtgta aatattattt gaattatccc tgtaactact attatttgca
      481 atgccagttt atttttaaag aaatttggta ttgtactatt taaaccaaat tctctacaat
      541 ttaatgcatt aaaaagggca ctgaacgcaa ataaagaaaa tataacagta ccttgttctt
      601 ctgatgtagc tcccaatata ttaaaagctg attgagtaaa tacaattgta attataagaa
      661 tagcatttat aataatattt acaaacatag acctagctat tattccagaa tgtctattta
      721 ttggttttct tttcaataca taatctctaa caggttctaa tcctaatgct agggctggag
      781 gaccatccat tattatattt acccataata attgtattgt agtaaatggc atatccttac
      841 cagtaagttg agatattata gcaattataa atgctactat atttacagta agctgaaact
      901 gtataaatct ttgaaagttc tcatatatac ctcttcccca ttttattccc tctactatag
      961 tactaaagct atcatcagtc aatattatat ctgctgcatt tttagatact tctgttcctg
     1021 aaatacccat tgcaattcca acatctgctt gactaagagc tggggcatca tttataccat
     1081 cacctgttac agctacaact tcgccacttt tttgaagcgc tgaaactatt ctcatttttg
     1141 tatctggttt acttcttgca acaatagata ttcctttaat ttcttctctc agttctttat
     1201 ctgtcaatac atctatatat gttgcttcaa ctgccttttt cccatcattt aataatccta
     1261 attcatttcc aatagccgtt gctgtattta tattatctcc tgtaagcatt tttacatcta
     1321 ctcctgcatt aaatgcttta tcaatggaat ctttgacccc ttctctcaat gggtccacta
     1381 ttcctacaaa tccactaaaa actaaattat cttccttcat ataacttctt gaatttccta
     1441 ttaaattcaa ttcaccttct gaagtcattg caacttctgt tttagaatta ctcatttttt
     1501 tataagcaaa tcctaatgtt ctcatagatt taatttgtaa cttcttaatc tcatctaaaa
     1561 tacttttttc tacttttgga gttattggaa caatattttt tccttgttgt acataagaac
     1621 atttttttaa taatacttct ggagcacctt ttgaaagtaa tatatctgaa tcctcttgac
     1681 gaattaatgt tgacatcttc ttttcttctg aactaaatgg tatttgtgat atcaaatacg
     1741 tttgttttct catttcatta tagttcttat cattatgata taaaagtaat gcacactctg
     1801 ttgcacttcc tatatattta aatgaattat cttctttttc tatatctgca gttgaattta
     1861 ctatacagtt ttgttcaaaa tagctgttac tttgatactc tcctccactt atatatttac
     1921 catcaacata tgctacctct accatcattt tgttttgtgt taaagtccct gttttgtcgg
     1981 aacatattac agaaacagaa cctatagttt cacaggcttc tttctttgtt actaatgcat
     2041 ttatctttgc catcttttgc attgttatag ctaaggtaat atttatcata gttggcaaac
     2101 cttctggaac agttgcaact attaatgcaa tacaaaccat atatgcattt ttagcaggtt
     2161 ctaatgattg taagaaagat aatatactgc ttgtatctaa aattaacata ccttttctct
     2221 gcatctgtat aatcataaac atacacaata aagtagctat tacgcctgat attcctgcta
     2281 ttttagcacc caacttccct aatttaattt gaagtggagt ttgctggtct tcatttgata
     2341 aattctgtgc tatcttaccc atctctgtct tatcaccagt atgagtgaca accattatgc
     2401 ccctaccata tgctacaaga gtccctccaa ataccatatt gacttgcttt gcaggtattg
     2461 catcctgttc gattatttct gttttagaat aaactacttc catatctaat acagcatcag
     2521 cattcttagc tacatcatca gattctcctg ttagcatatc ttctctaagt ttcaaattta
     2581 tagactgtat aagtcttcca tctgctggta tcatatctcc agtttctata tatacaatat
     2641 ctcctggtac taaatcattt tttgatattt taattatctt tccatttcta agtactttta
     2701 cttctatatt ttctgttaat tttgacaggg cctgagctgc ttttttagat tttccttcag
     2761 ttattatgcc tatagaaatt ccaataaaaa tagcccctaa aatacctaat gcatcatgta
     2821 attctcccac aaaagaactt attactgctg ctcctatgag aattaatatc ataggttctg
     2881 tcaaactttc tcccaactcg tcccaaaatg ttctaccttc ttttatagta aattcattta
     2941 atccatatct aagcttcctt tcttctactt cattatcatc aagtccaatt tctggatttg
     3001 tttttaagta ttttaatact tctttggtag gtttattata atacctcata actttatccc
     3061 ccttgttaat tatctataag gaattatatg cttatgatag cttgtatatt acttttcaaa
     3121 aaaaataagg tgttgattaa tcaacacctt atagtggtta tatattatta aattttatgt
     3181 tacaggcttt ataaattgtg agctatacaa atcaaaatat ttaatcttca aaataccctt
     3241 ctggatataa tgggaactta gatataagtt cttgtgcctc tgctttacat tcatctaata
     3301 cttctttatt atcaatattt tctgctacct tattcattat tcctgctatc tgtataactt
     3361 ctttttcttt cagacctctt tgagatattg atgtaagacc tatacgaaca ccactagtta
     3421 caaatggtga ttgtgggtcg tcaggtataa catttttatt aactgttatt cctacatatt
     3481 caagagcttt ttcaaattgt tttcctgtaa gattcttact tcttaagtca accattacta
     3541 tatgattatc agtacctcca cttacaatac taaaaccatg tgaaatcagt tcagaagcta
     3601 gagtctgagc atttactaca acttgctcca ttatcttttt aaatttatct gtacttaaat
     3661 ataatagtga aaaggctttt gctgcaactg tattaagatg tatagaccct aatgttcctg
     3721 gaaaaacacc tttatcgagt ttctttgcat gttctgcttt acaaagtaca ataccacttc
     3781 ttggtccaca gaaagtcttt gtcgtagatg aagaaacaaa atctgcataa ggtacaggac
     3841 taggtattac tttcgctgcg actaatcctg caacatgtgc catatctacc ataaagtaag
     3901 ctcctacttc ttttgcaacc tttgatattc tttcataatc tattaatctt ggatatgaac
     3961 ttgcccctgc aattataagt tttggtctac attcttttgc ctttgcttca agagcatcat
     4021 aatctatacg tccagtattt gggtctaccc cataaaattc atatttataa atgtcatgta
     4081 aaaaattaac tgcgctacca tgtgtcaagt gtccaccttg gtcaagacgc atacttaaaa
     4141 cagtgtcatt tggttttaat attgatgaat atacacaata gttagctgta gaacctgaat
     4201 acggttgtac gtttacatgt tctgctccaa atacttcttt tgcacgtttt attgctaggg
     4261 tttcaagttt atctgcttct tcagaacctg cttgaaaacg agcccctgga tagccttctt
     4321 ctgttttatt tgtaaataca catccactta attcaagtac ttctgttggt gctgtacttt
     4381 cagaagctat catctcaata ttgtgttctt gtctaaccaa ctcatcagct acaatcttgt
     4441 ataactctgg gtctgaaatt tttgttgttt ttaacataat aatcctcctc ttaattaaag
     4501 aatatatttt attagtttaa tcttcattga taaatttatt ataatggtct ataaaacgta
     4561 agatattcct ccaccaggtc ctaatggtat gccaataccc atgaatagta acaatattat
     4621 tatttctatt aatgtaaata ccactgagta tggaatcata ttcgatatga cagtacctat
     4681 acctatatct ttatcatatc ttctggcaaa ggccaatata actggaaaat acggaaaaag
     4741 tggacttatt ggatttgttg aagcatctcc aatacgataa gctatttgag ttaatgctgg
     4801 attaaagtct agtagcataa acatgggaac aaatataggt gctaaaatcg cccattttgc
     4861 agaagcactt cctacaaata aattgataaa tccagatagc aatataaatc ctattatcaa
     4921 tccatagcta ggtatccccg actctgctag taattttgct cctgttattg ataaaattgt
     4981 tcctaagtta ctaagattaa ataaattaat aaactgggat gctataaatg ctaggactat
     5041 atatccaccc atatcactca tactactacc catcatagat actaaatctt tgtcactttt
     5101 tatcttctta cttacttttc catacactaa tcctggagtt aaaaatataa tagtgattat
     5161 aggaactata cctttcatta aaggtgcatt actagatagt atagaaccag tctctggatc
     5221 tttcataaat gcattgtcac ctatacataa ggcaactatc actactacta caactaacaa
     5281 tgataataaa gcgtatttta tacccttatt ttcaagttca ctcaattcct gtatatcctt
     5341 atcatttgtt gaatcattat attttgtaaa acgaggagct ataaattttt cagttacaaa
     5401 tgctcctgca aaagttaaaa ctatcgctga tataaaagca aaataaagat tcattgctgg
     5461 attgccttgg taagatgggt ccatcatttg agctgctggt attgtaaatc cagctaacaa
     5521 tacatctgta gtactaagca taacatttgc tgcaaatccc cctgcaacac ctgcaaaagc
     5581 tgcaaacata cctacaagag gatgtcttcc aattcctaaa aacacaagtg ctgcaagtgg
     5641 tggtaatatt ataaatccta catctgctgc tgtatgtgat aacatacctg caaatacgat
     5701 tgctacagtt acaagttttt gtggaacttt acttataccc tttttcatta acacttccat
     5761 aaatccagat ttctcagcaa cacctgctcc aagcattgta accagtacta atcctaaagg
     5821 agcaaatgac tggaagtttg taactatgct acctaaatat gattgaatct gctctttctc
     5881 tagtagattt actaccttta caacttcttt tgtagaagga tgtatttctt ctactcctaa
     5941 attagatata actgctgaca atattacaac aattatacat agtaataaaa atatggttac
     6001 tgggtctgga agtttatttc ctgctctctc tacaacattt agtgccctca tcaataatcc
     6061 actatctttg tttttttcct gtgaattaat tttcctcaaa ctaatacccc ctcataaact
     6121 ttatctccag aatgctctat cttaaatttt attttttaat ttatccatat aatttataac
     6181 agactgacat agtacatttg tacctaccaa tatagaattt ttatcagctt taaatttagg
     6241 attatgccac tgatatatac aatcttgttc tttattgcct acccctaacc agtaaaaaaa
     6301 tcctggggcc ttttccataa atatagaaaa atcttctcct cctgtagaag gtattggatc
     6361 aactatagca tcttctgaat acaaatcaca cacagactta caagctattg tgtacatctc
     6421 tttactattt ataacagctg gtaaatcaaa taaataatct agctttgcag ttgcttgata
     6481 tccacgagct atctcacaaa ctatattttc taaattttct gatattttct ttcgtacttc
     6541 attactaaag cttctacatg tacctgtcat atctaccttt tctgaaatta cattatttgc
     6601 agtccctcca tttatagtac ctattgatat aacagctgat tctaatggac ttatgtttct
     6661 gcttactatg gtttgaatac ccattataat tgcacttgat gctactatag ggtctattgt
     6721 tctgtttggt atacctccgt gacctccaaa tccatttact tctatcttaa tagtgtctac
     6781 tgctgccatt aacccaccta acttaacacc tatttttcca caaggtatat ctggatgatt
     6841 atgaagtcca aatatagcat caacttttgg attttccatt actcctcgtt ccactagcat
     6901 cttcgcacct ttattgactt cttcggctgg ttgaaatata aactttacat ttccatgaag
     6961 actatctcta atatgagaca atacatatgc acatcctaca aggcaagatg tgtgtatatc
     7021 atgcccacat gcatgcatta taccatcata tctggattta tactcaatat caacctcttc
     7081 atatattggc aatgcatcaa tatctcctct cagtgctact gtgggaccat catacttccc
     7141 ttttaataat ccaacaactc ctgtttccat tcctaaatct aatatttcta tgtctaaact
     7201 tactaataaa tcttttatag cttttgtagt cctaaattct ttaaaactta attcggggtg
     7261 catatgaata tctgtaaaaa aatctaaaat atcttcttta tacttttctg agattaaaat
     7321 aagttcatgc atactaacac ctctccaaaa ttttattttt ttatttttta taatttaact
     7381 acaaattata taagttaata atctttataa agatattttt tataaattta attttagttt
     7441 atatttatat tttactatat tattttaatt aaatatatta tcatgttttg caatttattt
     7501 aactattttg actttttaaa ttcggattat ataaaaaatt tctttaaata aaaactatta
     7561 ataggtgact ctattcctga ctttgtgttg atttttttct acatgtaatt gctttataca
     7621 ccatacagac taaaattaaa agccccatat aacctgtata tggatataaa atgtttacta
     7681 atttaccaaa cggtaaaaaa ccacctatac aagccaaaat agctattact gttgttacca
     7741 atttaaattt aggatgttca tcttcaacaa ttctattggt tacagaccat aaaagtggaa
     7801 cagctgttgt atagatacct aatattaatg caactgaaaa acatacacct acaataggag
     7861 atatattatc tgctaaataa agtgatggta tttctttcat atacaaattt cctatatctg
     7921 aaatcatagc caaatacata actataccag cagtcataaa cactattcca cctaataaac
     7981 ctccccaaaa agcatccttt ttactttcaa tagaagctcc cattcctgtt aaaaaaacaa
     8041 tcattacaat aatatttaat gtattatata ttacaccaga aacgattgga ctttctgatg
     8101 cttttgtaac ttcaatattt ttcatagctt ctccaaccat agataaacca tcaaaatgct
     8161 taaacaagct tattaatcca acagcaattg taaatactgc tatcactggt ccaatacgtc
     8221 caagtatgct tactaatttt tctaaaccca tagacactgt aattaaagta gcaacagcca
     8281 tcaatactct tcctaagtaa ggatttatac catagtattc agttaaagtt gagcctgcac
     8341 ctgaaatcat tactataaga atcccaaata aaaatattgg actaaaccat tcgtaaaatt
     8401 ttcctaaaat cttcccacaa taaaaagtgt aaattcttgt tggttcttta agttttaatt
     8461 ctctacctct agttataact tcagcaccaa gaaatgaaaa caataccatt gatattaatg
     8521 ctccaattaa acccatatac ccttgtgagg caaaaaattg tagaatttct tgaccagtag
     8581 caaacccaga accaattgct gttgctagat aagcgcctgc aatacagata atatttttca
     8641 tattcacttt gtctgacata ttaaattacc tccattataa agttaccttc ctttaagaaa
     8701 tataaactct cagtgaatta aatgaaaatt ctgtcttttt gaaacagtat ttaattcact
     8761 ttttttattt tgtatatctt tttcttttta tttatatata tttttttata taaataggta
     8821 cagcttttat aaggtttaat attattaaat atgtttaaag cgctttaatt tttatcataa
     8881 ttaaagtata cttattcaga cacactcaaa caattatcta tattgacttt ttatttatca
     8941 atttcaattt ttagtgttca gtttataaac ttttggagtc attccttttt ctatcttaaa
     9001 gtttttataa aagttattta ggctattata tccaactatt tctgcaatct catttatact
     9061 ttttgtagta ttttcaagta attcaataga
//
LOCUS       DAEPOZ010000147         8952 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.147, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000147 DAEPOZ010000000
VERSION     DAEPOZ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8952
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.147"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            229..876
                     /gene="cas5"
                     /locus_tag="KR440_002585"
     CDS             229..876
                     /gene="cas5"
                     /locus_tag="KR440_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HBH0699623.1"
                     /translation="MILNKIEIIGDMAHFKIPMHSKIQRTYEIPPISTVVGILKNIYG
                     EEIDDFILGYVIYHDGTFKDLQTIYKEINPNVKTLTDSDRFQTDVCPIEYLINPKLII
                     YTNIDKNIEFNEPLCLGKTNCLAKVLSLSEIEVNLIDKKAIGYNQYTDINIGDGMIKR
                     INTLTEYNEKKGYYDIYSSMVRENDEFEYGKYYDEDEQQNIFLWKWKKGGVIKCI"
     gene            867..1631
                     /gene="cas6"
                     /locus_tag="KR440_002586"
     CDS             867..1631
                     /gene="cas6"
                     /locus_tag="KR440_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBH0699624.1"
                     /translation="MHLVRRHLILTVDNEVVLDYNYQYELMKRIYEAIEINDKRKALS
                     LHNEGYKVDKKVFKLFNYTIMFENAKYLKEGIHLNPQTKIKLILSGYDDILNNIIKGF
                     IKCKVFKLNNIEFKVSDIEEDSKKNFNNITLYKVRSPIVASLYDLKSRKQVYLNPMQE
                     EFYKALHDNLGNKYKLIHNKEYTGELYFDIEDVLAVKKKYITNIKGKGFIIGYTDFEI
                     FVQANKDMQEVIYYCGLGEKNSIGMGLLTYITSRRA"
     gene            1633..2799
                     /locus_tag="KR440_002587"
     CDS             1633..2799
                     /locus_tag="KR440_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699625.1"
                     /translation="MDRSYNVFVDNGLYVLAYYLNKDINDITYQDIENSIDLMSDKIE
                     EFVSCEKYSNLKSMCFSNSTLTQPKGKVTLNEKLKGFVKNQGDEYCSLCGQYKAKVKI
                     EDKEYNIGRSYMPNLVANTFYNFSNNLQGLNVCPYCLVLTMYSILNCRVSRYAFLYNS
                     TSSEFMEDYTFHIQKENLTDVELGAKKEKEKYSIVKLLESLVCKYNLFNGDIEQYMFN
                     NSGQSQDIQVNRIKNKYVNLLIKLQEKALLSNFEKLHLDRYILNGTLESNYLREVYRV
                     KKEEKMDEKEQKNFDELVIFLNREVNVLKKVKIDIIKKLTDKLKTSDLDIDKIYKELK
                     MLGKFYAYEDWLVNLNEMYYEKTGEKLFEAEDYIELDDIRSFRQIKNLILVSLI"
     gene            2820..3890
                     /locus_tag="KR440_002588"
     CDS             2820..3890
                     /locus_tag="KR440_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HBH0699626.1"
                     /translation="MENKNSNRMLNIGYAIEVLCGRLNGSSSEVEKKNNSYKNSTYTK
                     KIGSRSRISSQCQKYNQKLFMEQVCGYEQNVKTKDGKQVKLVPNPFKYATDDIFGFMM
                     AEKLSIEEDVYKELSDEEKEIYKKNKKKYEVNITKKRKSRWQMNSLINVSNRKVEWEW
                     NVCGTTGDSMPYQVEVYSGVHTNIANLNIGKIGEYDISDIASEHRDYTTLEAKSIGVE
                     KLDKEEKFKRIEALLKSLEYLSVEGNKTNHLTDTKPKFVILGEYSWGNNVFQGIIKEN
                     GLDVEMLKESIEQNEEFRKSDIWIGINKFYDDTFNEIEVNNEKEQIKKYLEEEFEEYD
                     FMKISNVHQAFESYLKYLKETL"
     gene            3901..5886
                     /locus_tag="KR440_002589"
     CDS             3901..5886
                     /locus_tag="KR440_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBH0699627.1"
                     /translation="MNNLYEYKAKSDGTSIDKHNEDMNYIMNQLVSIYNISNDMKNDL
                     YEAIVCHDVGKVVKSFQDNIESSTRRVRHELLSASIKYLNRGQKLAILTHHKDIKFLE
                     GQITNSIYEIELKELKELLDIEVEDIRPFIKEISRARNDDTRKLENILLKGYLQYCDH
                     IASAGIKSIDKGFNSIKDFSIPKGVSYNSIQEKVLNMDKNEDIMIMAMTGLGKTATSL
                     YWSNLVQNEYKSKRVYYILPYTASINSLYENLYNRNISVSMLHSKAPYFLNKISDEED
                     IKQLYQIYKKSVKQINVCTIFQLVKSVFSCKRFEMLLSQLKDSIFIVDEIHCFDTRVL
                     CYTLEFLKYLKENLNVKICIMSASIPTCLKKLIQERLQISTIIEPDSKDLIFRHRLNR
                     LSEGILDSIHKIENDLMQGKRVLICVNSVDLSQELYGRLQKYNPKLIHGRFNTRDREQ
                     AEKGIEESFLLIGTQSIEVSLDISYDVLYTELAPLDSLLQRFGRVNRRGDNAELSNIY
                     IYDYDRKLNIYDEDIINRTDIVISDIIHNDNSIVDESKVPSYLDNVYLDIDMDKYNKH
                     KVALEIIMKNMRLGVWNKSANNDMLNNDTISVVPECLLEEYKLYIDKKMYLEASSLFV
                     NVSVKRYNYNKKMFYECGDIVVTSYSYNDKLGLIFDC"
     repeat_region   6139..6761
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=6139..6167
                     /rpt_unit_seq="gtttaaattacactaagttagttataaat"
     gene            7206..8762
                     /locus_tag="KR440_002590"
                     /pseudo
     CDS             7206..8762
                     /locus_tag="KR440_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003444896.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
BASE COUNT         3832 a          816 c         1457 g         2847 t
ORIGIN      
        1 atacatagaa atattttaga aaattacaag tactatattg aaagtttgaa ggagaacctt
       61 gatttaaggt aagtgtaaag tttttagaaa cttcactaaa ataatctaat taagttatat
      121 atttgttact tttaggaaaa taatggtaaa aattaaaggt ttttataaat gaatgtctaa
      181 ttttataaat aaaatggagt attttaacta aatatttgag gagataaaat gatattaaat
      241 aaaatcgaaa taattggaga tatggctcac tttaaaattc ctatgcatag caagatacaa
      301 agaacatatg aaattccacc aatatcaaca gttgtcggaa ttttaaaaaa tatatatggt
      361 gaagaaatag atgattttat attaggatat gttatatatc atgatggtac atttaaagat
      421 ttacagacta tatataaaga aatcaatcca aatgtaaaaa cattgacaga tagcgataga
      481 tttcaaactg atgtatgtcc cattgaatac ctaattaatc ctaaacttat catatatact
      541 aacatagata aaaatataga atttaatgag ccgctatgtt taggtaagac taattgttta
      601 gcgaaggtat tatctttaag tgaaattgaa gtaaatctaa tagataaaaa agctattgga
      661 tacaatcaat atacagatat taatattgga gatggaatga taaaaagaat aaatacattg
      721 acagagtata atgaaaaaaa aggttattat gatatatatt cttcaatggt aagagaaaat
      781 gatgagtttg aatatggtaa atattatgat gaagatgagc aacagaatat ttttttatgg
      841 aaatggaaaa aagggggagt aattaaatgc atttagtaag aagacatttg atactcacag
      901 tggataatga agttgtatta gattacaatt accagtatga gctaatgaaa aggatatatg
      961 aagctataga aattaatgat aaaagaaaag cactaagtct tcacaatgaa ggctataagg
     1021 tggataagaa agtatttaaa ttatttaatt acacaattat gtttgaaaat gctaagtatt
     1081 taaaagaggg aatacattta aatccccaaa caaaaatcaa attaatttta agtggatatg
     1141 atgatatatt gaataatatt ataaaaggtt ttattaaatg taaagtattt aaattgaata
     1201 atatagagtt taaagtttca gatatagaag aagattcaaa gaaaaatttt aataatataa
     1261 cactttataa agtaaggtca cctattgtag caagtttata tgatttaaaa agtaggaaac
     1321 aagtatactt aaatccaatg caagaagagt tttataaagc attacatgat aatttaggaa
     1381 ataagtataa gcttatacat aataaagaat atactggtga actatacttt gatattgagg
     1441 atgtattagc agtaaaaaag aaatatataa ctaatataaa aggaaaagga tttataattg
     1501 gatatacaga ttttgaaata tttgtacaag ctaataaaga tatgcaagaa gtaatttatt
     1561 attgtggatt aggtgaaaaa aatagtatag gaatggggtt actaacttat ataacttcta
     1621 ggagggctta atatggatag aagttacaat gtatttgttg ataatggtct atatgtgttg
     1681 gcttattatc tgaacaaaga tattaatgat ataacttatc aagatataga gaatagtata
     1741 gatttaatga gtgataaaat tgaagaattt gtaagttgcg aaaaatactc taatttaaaa
     1801 agtatgtgct ttagcaattc tacgctaact caaccaaaag gaaaagttac tttaaatgag
     1861 aagttaaaag gttttgttaa aaatcaagga gatgaatatt gtagtttatg tggtcaatat
     1921 aaggccaaag tcaaaattga agataaagaa tacaatatag ggagaagtta tatgcctaat
     1981 ttggttgcaa atacatttta taatttctcc aataatttgc aaggtttgaa tgtatgtcca
     2041 tattgtttag tattgactat gtattcaatt ctgaattgta gagtaagtag gtatgctttt
     2101 ttatacaatt ctacatcaag cgaatttatg gaagattata ctttccatat tcaaaaagaa
     2161 aatttaactg atgtagagtt aggagcaaag aaagaaaaag aaaagtattc aattgtaaaa
     2221 ttattagaaa gtctagtgtg taaatataac ttatttaatg gtgatataga gcaatatatg
     2281 tttaataact ctggtcaatc tcaggatatt caggtaaata gaataaaaaa taaatatgtg
     2341 aatttattaa taaaattaca ggaaaaagca ctactatcaa actttgaaaa gttacatttg
     2401 gataggtata tcttgaatgg tactttagaa agcaattatc tcagagaagt atatagggta
     2461 aaaaaggaag aaaaaatgga tgagaaagaa caaaagaatt ttgatgaact tgttattttt
     2521 ttaaacaggg aggtaaatgt attgaaaaaa gtcaaaatag atataataaa aaaattaacg
     2581 gataaattaa aaactagtga cttagatata gacaaaatat ataaggagct taaaatgtta
     2641 ggaaaatttt atgcatatga ggactggtta gttaacttaa atgaaatgta ttatgaaaaa
     2701 actggagaaa aactatttga agcagaggat tatattgagt tggatgatat aagaagcttt
     2761 agacagatta aaaatttaat attagtaagt ttaatttaat aatattatgg agggttaaaa
     2821 tggaaaataa aaattcaaat agaatgttaa atataggcta tgcaatagaa gttttatgtg
     2881 ggcgattaaa tggaagtagt agtgaagtag aaaagaaaaa taattcatat aagaattcta
     2941 cttatactaa aaaaataggg agcagaagta gaatctcaag tcaatgtcaa aaatacaatc
     3001 aaaagctatt tatggaacaa gtttgtggtt atgaacaaaa tgttaaaact aaagatggga
     3061 aacaagtaaa attggttcca aacccattta aatatgcgac agatgatata tttgggttta
     3121 tgatggcgga aaaattatct atagaagaag atgtatacaa agaattatca gatgaagaaa
     3181 aagaaatata taaaaaaaat aaaaagaaat atgaagtaaa tataactaaa aaaagaaagt
     3241 caagatggca aatgaactct ttaataaatg tatcaaatag aaaagttgaa tgggagtgga
     3301 atgtttgtgg aactacagga gatagtatgc catatcaagt tgaggtatat tcaggggtac
     3361 atacaaatat agcaaatctt aatataggaa agataggaga atatgatata tctgatatag
     3421 caagtgagca tagagattat acaactttag aagctaaatc aattggagtt gaaaagttag
     3481 ataaagaaga aaagtttaaa agaatagaag ctctcctaaa atcactagaa tacttaagtg
     3541 tagaaggtaa taagacaaat catctaactg atacaaaacc caaatttgtt atattgggag
     3601 agtattcatg gggaaataat gtatttcagg gtattatcaa agaaaatggc ttagatgtag
     3661 agatgttaaa agaatctata gaacaaaatg aagaatttag aaaaagcgat atatggattg
     3721 gaataaataa attttatgat gatacattta atgagataga agttaataat gaaaaggaac
     3781 aaataaagaa gtatttagaa gaagagtttg aagagtatga ttttatgaaa atatcaaatg
     3841 tacatcaagc ttttgaaagt tacttaaaat atttaaagga gactttataa aggatatatc
     3901 atgaataatt tatatgaata taaagctaaa tcagatggga caagtataga taagcataat
     3961 gaagatatga attacataat gaatcaatta gttagcatat ataatatatc taatgatatg
     4021 aaaaatgatt tatatgaggc aattgtatgt catgatgtag ggaaagtagt aaaaagcttt
     4081 caagataata ttgaaagtag cacaagaaga gttagacatg agttattatc agcaagtatt
     4141 aagtatttaa atagaggtca gaagttagct atattaaccc accataaaga tataaagttt
     4201 ctagagggtc aaataacaaa ttctatttat gaaattgaat taaaggaatt aaaggaacta
     4261 ttagatattg aagtagaaga tataagacca tttataaagg aaatatcaag agcaagaaat
     4321 gatgatacta gaaaacttga aaatatatta ttaaaaggtt acttgcaata ttgtgaccat
     4381 atagcttctg cgggaattaa aagtatagat aaaggattta attctattaa agattttagt
     4441 ataccaaagg gtgtaagtta taattcaata caagaaaaag ttttaaatat ggataaaaat
     4501 gaagatataa tgataatggc tatgacaggt cttggaaaaa cagcaacatc attgtattgg
     4561 agtaacctag ttcaaaatga atataaaagt aagagagtgt attatatact tccttataca
     4621 gcaagcataa attctttata tgaaaattta tataatagaa atataagcgt aagtatgtta
     4681 cacagtaaag ctccatattt tttgaataaa attagtgatg aagaagatat aaaacaactt
     4741 taccagatat acaaaaagag tgtaaaacaa attaatgtat gtactatatt tcaattagta
     4801 aagtcagtat tttcttgtaa gagatttgaa atgctactat cacaattaaa agatagtata
     4861 tttatagttg atgaaataca ttgttttgat acaagagtat tgtgttatac attggaattt
     4921 ttaaaatatt taaaagaaaa cttaaatgta aaaatatgta taatgagtgc aagtatacca
     4981 acttgcttaa aaaagttaat acaggaaaga ttgcaaataa gtacaattat tgaaccagat
     5041 agtaaagatt taatatttag acatagattg aatagattaa gtgaaggaat acttgatagc
     5101 atacataaaa tagaaaatga tttaatgcaa ggaaaaagag tattaatatg tgttaattca
     5161 gtagatttat ctcaagagtt atatggaagg cttcaaaaat ataatcctaa attaatacat
     5221 ggtcgattta atactagaga tagagagcag gcagaaaaag ggatagaaga aagcttctta
     5281 ttgataggta ctcaaagtat agaggtaagt ttagatataa gttatgatgt tctttataca
     5341 gagttagctc ctttagattc attgttacaa aggtttggta gagtaaatag aaggggagat
     5401 aatgctgagt tgagcaatat ttatatatat gattatgata gaaagcttaa tatatatgat
     5461 gaagatataa taaataggac tgatatagta atatcagata taatacataa tgataattct
     5521 atagttgatg agagtaaagt tccatcttat ttagataatg tctatttaga tatagatatg
     5581 gataaatata ataaacataa agttgcttta gagattataa tgaaaaatat gaggttaggg
     5641 gtatggaata aatctgctaa taatgatatg ttaaataatg atactattag tgttgtgcct
     5701 gaatgtcttt tagaagagta caagttgtat attgataaaa aaatgtattt agaagcgagt
     5761 tcattatttg taaatgtatc tgtaaaaaga tacaattata ataaaaagat gttttatgaa
     5821 tgtggagata tagttgttac aagttatagt tataatgata aactaggtct aatatttgat
     5881 tgttaagaga attatagtat aactaattaa aatattttat tgagaatttg ttataattta
     5941 tatcgactac aaattttata tgttacaata gaataatttt gcagtgagcg aaaaatatgt
     6001 gtaaatggct gtgagttagt gatggcaagg tttataactg aatttttgtt tttagctaaa
     6061 aacactactg gtgcctcact gcaaattaac tattttaata accctacaag tgttgtaatt
     6121 actatagttt tatttggggt ttaaattaca ctaagttagt tataaatatt ccggaaagta
     6181 cttttgctat ttgattcatt ctacccgttt aaattacact aagttagtta taaatttcac
     6241 taattttagt tacaactatt acaagtccag ccgtttaaat tacactaagt tagttataaa
     6301 ttatgtgtaa tgtattttaa tgttttctag caacaatgtt taaattacac taaattagtt
     6361 ataaatttta taaattctat gaataatttt aaagagaggt ttcgtttaaa ttacactaag
     6421 ttagttataa attgtacatt tgacatagaa gcttcaaaat ccatcccagt ttaaattaca
     6481 ctaagttagt tataaatgta acagcacgaa tgatggcaca gacctatctg caatgtttaa
     6541 attacactaa gttagttata aatagaagta cacttacaag taagataaaa gacccaggta
     6601 cagtttaaat tacactaagt tagttataaa ttctgtagct atatgtggaa gtaattttag
     6661 tagagaggtt taaattacac taagttagtt ataaattatt gaaggggtga gagttgtgac
     6721 taggcactac aagtttagat tacactaaat tagttgtaaa tttacttatt cattaagaaa
     6781 cttaactgag agtttgattt atattattga attagcaaga gttttagaaa gtagaaaatt
     6841 ataccccaat aataccccaa ccacaaaaat aaataataaa aaatatccaa aaaaattagt
     6901 ataacatcat aactaaatag ctatatacct acacttacga aaaccaacaa aacaatcaaa
     6961 taaccacttg tagactcccc tcgtctccac caattttgaa aatgaaaata atagaataaa
     7021 aggaattact tatgtttgga ctaagtaatt ccttttattt tgcttaaaag cacacaatta
     7081 gcacacaact agaaggttat ataaataaaa aggctgtcgc actagaaaat agtgctacgc
     7141 tcttttttgt ataaaaaaat aaatcccaag ttcaaaagaa cctgggattc ttgtataata
     7201 actgtatgct aaaacaaaat actacacaaa ttgattatac tacttatggt gataattatc
     7261 aatttaagct accactaaat attgattaca tgattccaaa agatgattct gtaaggttat
     7321 taagttaaat tattgaggag atgaatttag aaaaattata taagacttat tctcgaatta
     7381 gatgaaatag agtaatccat agacaatttt tgaaagttat aatatataca gatatgaact
     7441 gtatttattc ttcgagagaa atagaaagaa catgcaaaag agatattaat tttatgtacc
     7501 ttctaggcga aacctccact ccagaccatt caacaattgc aaaatttcgc agtatacatt
     7561 tttaactaat aggcaaaagc ttgattgcac agtttacaaa ctttcttgga gataataatg
     7621 aaatttcaaa agacacaata tttattgatg gtacaaaaat agagtcatct gctaatagat
     7681 atacttttgt ttggaaaaaa gctgtaaata aaaatatggc taaaatggtg aataatatat
     7741 ctgacttcat attgaaatgt gagattgatg ttggcattaa aatagtttat gaagataaaa
     7801 ttaaaatcta tcatttaaag aaactaaaaa aaatatcaaa agaagcaaat gtagcattta
     7861 ttcatggaaa aggaaataga aaatctacta aaaaccttca tataatgggt gaacgaaata
     7921 gttgttctaa aacagataac gatgaaactt ttatgagaat gaaagaagat catatgaaaa
     7981 atgggcaact gaaaccagca tataatatac aatttggtgt agattaagaa tatatagttt
     8041 ggctaaattc aggaaatcaa actgctgata ctacaacatt aataccattt ttaaaatcta
     8101 tagaggaata tactaagttt aaatatccta aagtaatagc tgatggtgga tatgaaagtg
     8161 aataaaacta tgcattttta gagcataata atcagttatc tttcataaaa cctgcaaatt
     8221 atgaaattat taaaactaga aaatataaaa cttatattgg atgtattgac aatatggatt
     8281 atgaagaaga aaataattat tatatttgta caaatgagaa aaaactattt gctaataaaa
     8341 taataaatag gaaatcaaaa acaggatata aaagtgaaat aacatgttac atttgtgaag
     8401 agtgtagtaa ttgtcaatat aaaagcaatt gtattaagag aagtaatagt aaggttcctc
     8461 ttaaaaatag gactaaaaac ttacaagtat ctaagtcgtt tcatgaaaaa agaaaagaaa
     8521 acttaaagag aattatgagc ctcgaggggg atgaacttag agtgaataga agtatacaag
     8581 cagaaggagc tttcgctcaa gtaaaataaa atatgaaact tacaagattt ttaagccaag
     8641 gtaaagaaaa tgttttataa ggatatatta ttgtggcgat agcttataac gtttctagac
     8701 tacacaataa aatagtaaaa aataaacgtg gaatatatct ttatcattta aaaaaatatt
     8761 ctaaaattat tatttataat caaatattat ctgaaaaaat ggtattttta tatctttatt
     8821 tgtcatgcat aattttactt agaacagaaa ctttatataa aacaattgaa aatataccta
     8881 ttaagataat tagaaaagag ctctcacaga acaaatttaa tttgctcatg caacagcccc
     8941 atattgatat ac
//
LOCUS       DAEPOZ010000148         8941 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.148, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000148 DAEPOZ010000000
VERSION     DAEPOZ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8941
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.148"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            127..1035
                     /gene="trxB"
                     /locus_tag="KR440_002591"
     CDS             127..1035
                     /gene="trxB"
                     /locus_tag="KR440_002591"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBH0699628.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(1125..2948)
                     /gene="typA"
                     /locus_tag="KR440_002592"
     CDS             complement(1125..2948)
                     /gene="typA"
                     /locus_tag="KR440_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBH0699629.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            3160..3402
                     /locus_tag="KR440_002593"
     CDS             3160..3402
                     /locus_tag="KR440_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699630.1"
                     /translation="MLEVTKIIIKYFASESDTFIKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            3550..3741
                     /locus_tag="KR440_002594"
     CDS             3550..3741
                     /locus_tag="KR440_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HBH0699631.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
     gene            4032..6533
                     /locus_tag="KR440_002595"
     CDS             4032..6533
                     /locus_tag="KR440_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBH0699632.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVRNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNVLAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            6693..7361
                     /locus_tag="KR440_002596"
     CDS             6693..7361
                     /locus_tag="KR440_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0699633.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            7345..8622
                     /locus_tag="KR440_002597"
     CDS             7345..8622
                     /locus_tag="KR440_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBH0699634.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEKSELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
BASE COUNT         3520 a         1052 c         1523 g         2846 t
ORIGIN      
        1 atgtaaaata aagttaatta ataaaaaata atgttttgtt tgtagtatat atggataata
       61 atagtaaagt ataagaatga tgagaataat atataagaaa taataatata aagagaggta
      121 aacattatgg tagatatcat tgtaattggt gctggaccag caggtttaac ttcagcaatt
      181 tatgctatga gagcagggtt aagcgttaca gtatttgaaa aaaatattta tggaggtcaa
      241 gttgcaagta cctctgaggt agaaaattat ccagcagttc aaaagatttc tggggtagaa
      301 ttttctaata atatatacaa tcaagcagtt gctcaaggtg ttgatattca gtttgatgag
      361 gttgaagaga taaatttaga gggaaaagta aaggttgtaa aaacttcttc aggtgaacat
      421 aaagcaaagg ctgttattct tgcaaatgga gttgaaagac gtaaattagg ttgtgcaggg
      481 gaacaagaat ttacaggtag aggagtatct tactgtgcaa catgtgatgg agcctttttt
      541 aaagataaag aagttgctat agtaggtgga ggaaatactg cactagaaga tgctttattt
      601 ttagctaata attgtacaaa agtgtattta attcacagaa gagatagttt tagaggtgaa
      661 gaagtgttgg agaaatctgt gaaagcaaga gagaatattg agatacttta cagtcatggt
      721 gtagaaaaaa tagaaggaga aaagactgta tcgaaaatag aagtaaaaaa cttaaagact
      781 gaagaaaaaa gaactataga tgttagtggt atttttattg ctataggtct taaacctaat
      841 aataaaatgt ttgaaaatgt tcttgatttg gatgaaggtg gatatataat ttcagatgag
      901 tcttgtacaa catcagtaga aggtgtatat gtagcaggag atagtagaac taagttttta
      961 cgtcaaataa taacagcagc atcagatgga gctattgcag ccgttcaagc agctaattat
     1021 ataaatgttg aataatataa atttcaaata aaaatagcca tataaactga aataattttt
     1081 cagtactata tggctatttt gttaattaga ggtaaatgta cgttttattt accttgtcta
     1141 gatttgttat atcttaatct atcatgttca tttaagaatc tttttctaag tcttatagaa
     1201 tcaggtgtaa tttcaactaa ttcatcatct tctataaact ctagtgcttc ttcaagagta
     1261 aatattctag gaggagataa ttttatagca tcatctgaac cagaagcacg tacgttactc
     1321 attttcttgt ttttacaagg gttgactacc atatcatctt ttctggaatt cataccaatt
     1381 atcatacctt cgtaaacttc tacaccaggg tctacaaaca ttacagctct atcacttaag
     1441 gcattaagag aataccccat agtaacacca ggtccttgtg ctattaaaac accatttcct
     1501 cttgaaggta tttctccttt aaattcttca aatttttcaa atgaacgaac taaagtacct
     1561 tctccacgag tagcgtttat aaattcactt cgatatccta aaagaccacg agtaggtgct
     1621 aagaactcta tttttacata gtctccttct attgacatag actccatcat accttttctc
     1681 atgtttaatt catttataat agtaccagag taaacttcag gacagtttac aacaactctt
     1741 tctattggtt ccattagttt tccatcttct ttatgcatta aaacttcagg tttagaaaca
     1801 ccaacttcat aaccttcacg tctcatattt tcaagtagta tagataagtg aagctctcca
     1861 cgtccagaaa ctttgtatcc atcagtagta tcaagaggtt caactttaag accaacattt
     1921 acttcaagtt ccttttcaag tctatctttc aaatgtctag ttgtaacgaa ttttccactt
     1981 tttccaacga atggagagtc gtttactaag aagttcatag aaagagtagg ctcttctatt
     2041 tttatcattt ccataggtag tggactatct aaatcgcata tagtttcacc tatagatata
     2101 tcaggaatac cagctataac aactatttct ccactagtag cttcatcaac ttcaacttgt
     2161 tttaaacctt catacacaga aagttttgat acttttcctt tagatacaac agaatcttct
     2221 ctacatatag caacttgtgt attgttttta agagtacctt tgtaaattct acctatacca
     2281 agtctaccta cataatcatc atatgcaaga gctgatattt ggaattgtag aggttcatta
     2341 tcgtaattag gataagcttc aacgtgattt acaatagttt taaatagagg agaaaggtct
     2401 tcactatcat catccatttc aagtttagca ataccttgtt tagcaatacc atatattata
     2461 ggaaattcgc attgctcatc tgttgcattt aagtctacaa ataaatcgaa aacttcatta
     2521 actacttctt ccgctctctg gtctttttta tcaatcttgt ttataaataa tattggtttt
     2581 aaaccgaact ctaaagattt ttgtaaaacg aatctagttt gaggcattgg gccttcacta
     2641 gcatcaacta atagaataac tgtgtcaaca gttttcataa cacgttcaac ttcagaagaa
     2701 aagtcactgt gtcctggagt atcaactatg tttattttgt aatcttcata gtttattgca
     2761 cagttttttg aatatatagt tatgcctctt tctttttcaa ggtcgttact gtccataacg
     2821 cagtctttaa ctacttcgtt ctttctaaaa acaccgcttt gagaaaggaa agcatcaacc
     2881 aatgttgatt ttccagcgtc aacgtgggct ataactgcga tatttataat tttatgtttt
     2941 tgtgacattt ttgttgctcc cttcgattaa aataacttat taattatagc acaatatatt
     3001 attaggtaca aggaggaata tattaaattg tattaatata gtttaaattg gataaaactg
     3061 ttagttttgc atctgaaaat tattctgttt aaaaaaattt tttagacaaa taataatctt
     3121 aatagataaa aagattaata aaagagtaaa ggatgatata tgttggaagt aaccaaaata
     3181 ataattaaat attttgcaag tgaaagtgat acttttataa aagaagaagt ttgtaatttt
     3241 gaagaacttc ttaagtggtc tcataaattt attgaaggta aaaagataga agatgttttg
     3301 tatataaagt ctaaagaaaa ctataaagta aatgtgatta agtctttaga aattaaggaa
     3361 ttggaaatag tgagaaaaca acagatacca ccgtacatgt agtattaata atatataaaa
     3421 ttaatatgta atactcacaa taggatataa atggtatata tggtaataat ttatattaaa
     3481 aaaactttta taaagtttat atttgggtat aaaagtatta attagacatt aattaggagg
     3541 aacttaaata tgataagagt atgtccaaac tgttcaaatg tggatgtaaa taagctaaaa
     3601 actttggttg gagaagaaaa tgtaaagact ggttgtatag gtcagtgtag agcatttaaa
     3661 aaagaagctg ttggaataat agacaatgag cttgaaataa aggaaacaga agcagagttt
     3721 tttgaagcat gtaaagaata aattttaaat aaaatataaa ttataataaa tagagcatct
     3781 aaattgtata agcattaaac aatttagatg ctctattttt atatatacat ttaattatga
     3841 gtatactttt ctattaaata caaataaata taactaatac atttatttat taaaaaaata
     3901 ttgatttaat atacctatag ggggtataat aaaaatatag atttctaaat aagtaattta
     3961 tcaatattac atatcaaaat aaaacgttac aaattagctg aaaatatatt gaaagaaagg
     4021 aagattttat tatgagttca aataaagtgg tagaaagtta taaaattaca ggtatgacat
     4081 gtgcagcttg tgcaaaggca gtagaaagag taactaagaa gatggatgga gtatatgacc
     4141 aaagtgtaaa tatagctact gaaaaactta aaatagaata tgataattct aaggttaatt
     4201 ttgatgatat aaagcaggtt gtagaaaaag caggatatgg aattataaag gaagaaagta
     4261 ataaaaaaat tgatatgaaa attgatggaa tgacatgtgc agcttgtgca aaagctgtag
     4321 agagagtggt aaaaaaattg gatggagtag aaagtattag tgtgaatatt gcaacagata
     4381 aagctaatat agactatgac ccatcaaaag taaaattatc tcaaataaaa gcagctattg
     4441 aaaaagctgg atataaacca atagaagagg taagaaataa ggttgatgtg gatgaagata
     4501 aattgagaaa agaaagagaa atgaagtctt tatttgttaa atttatagtt gcaatagtat
     4561 ttgcagtacc tctgttttat atagcaatgg gacctatgat aataaagcct atagggccat
     4621 ggcctttgcc agaaattata aatcctatga caaatacttt taattatgca ttaattcagt
     4681 taattttagt aataccagta atgatagctg gttataaatt ttatataaat gggtttaaat
     4741 cacttttttc tttaagccct aatatggatt ctttagttgc aataggaaca ttagccgcat
     4801 ttttatatag tctatatacg acactacaaa tagccaatgg acaaattcaa ggtatgcacc
     4861 atcatcagtt gtattatgaa agtgcaggaa taataatagc acttatctta cttggtaaat
     4921 atttagaatc aaaatcaaaa ggaaaaacat cagaagcaat taaaaagctt atggggctac
     4981 aacctaaaac tgcaatagtg ttagttgatg gtaaagaagt tgaaactcct atagaagaag
     5041 tagagatagg agatatacta ttagtaaaac caggaactaa aatacccgtg gatggagttg
     5101 taatagaagg atatacatca gttgatgaat caatgcttac aggtgaaagt ataccagtag
     5161 aaaaaaatgt tggaagtaag gtgacaggag ctagtattaa taaaaatggt gttattaagt
     5221 ttaaggctga aaaaattggt ggagatactg ctcttgccca aataataaag ttagttgaag
     5281 atgcacaagg tacaaaagca cctattgcta aacttgccga tactgtatct ggatattttg
     5341 taccaatagt aattgcaata gctgtagttg catcattatt gtggttttta ataggaggaa
     5401 aagatatagt atttgtactt actatattta tatcagtact tgtaattgct tgtccttgtg
     5461 cattaggatt agcaacacca actgctataa tggttgggac tggaaagggc gcagaaaatg
     5521 gaatcttgat aaaaggtgga gaggcactag aatctgcaca taaagtaaat acagtgatat
     5581 ttgataagac aggtactata acagaaggaa agccaaaagt aacagatata gtattaaata
     5641 ataatgtaaa agaagaatat cttatcaaga ttgcttcaag tgcagaaaaa ggttcagagc
     5701 atcctcttgg agaagcaata gttaagtatg gagaagagaa gaatataaaa tttgaaaaag
     5761 ttgataattt taaagccata ccaggtgctg gtatacaagt tactattaat gatgaaagta
     5821 tattacttgg aaatagaaag cttatgaatg acaataatat aaagcttggg gatttagaag
     5881 aaaaatcaaa tgtattggca agccaaggta aaacacctat gtatatagct gtagatggaa
     5941 atttatcagg tataatagca gttgcagatg tagtcaaaga aagtagtaaa aaagcaatag
     6001 aaatacttca tgatatggga ataaaagttg caatggtaac tggagataat gcgaaaactg
     6061 caaatgctat agcaaatcaa gtaggtatag atatggtttt ggcagaggtt ttaccagaag
     6121 ataagtcaaa agaagttgaa aaactacaaa atcaaggaaa gtttgttgca atggtagggg
     6181 atggaataaa tgatgcccca gctcttgcaa aagcagatat agggatagca ataggaagtg
     6241 gaacggatgt agccattgaa tctgcggata tagtacttat gaagagtgat ttaattgatg
     6301 ttccaacagc aattaaactt agtcatgaaa ctataaaaaa tataaaacaa aacctatttt
     6361 gggcatttgg atataatacg attggtatac ctgtagcagc aggaatatta tatgtatttg
     6421 gaggaccttt attaaaccca atgattgcag cagcagcaat gagcttaagt tcagtttcag
     6481 tcgtatcaaa tgcacttaga cttaaaaact tcaaggcata taagagagat tagattaata
     6541 aattaaataa aatgtatcca aaagagctat ctttaaatta aaaggattaa tttacaaatt
     6601 aagatagctc ttttttcatg taaaattcac aacaataaga taaaatataa gtaatatacg
     6661 aaattttaaa taaaatagaa aggattgata gaatgaattc gtcaatactt gtaattgaag
     6721 atgattcaaa tatacaagag ttaatatcag aatttttaag tgcagaaggt tatcaagttg
     6781 atacagcaaa tgatgggttg gaaggtatac aaaagtttaa gcaaggaagt tatgatttag
     6841 taatattaga tataatgatg ccaaacttag atggttatgg agtttgtaaa atgataagaa
     6901 agtcatcaag cgttccaatt atatttttaa ctgctttaaa tgatgaagga gaccaactta
     6961 aagggtttga cttagaatgt gatgattata taacaaaacc attttcattt aatcttctta
     7021 taaaaagagt agaagcaatt ttaaggcgaa gtaacaagac tataaatgac aaatttatag
     7081 tttttgaaaa attaaaatta gatttaaaca catatattgc agagatagat ggagaaccta
     7141 tagaacttac actaaaagag tttaatatat taaaagcttt gatagaaaag tatccacaag
     7201 ttataactag ggaaggtctt ttggatagta tatggggtta tgactattat ggagatacta
     7261 ggattgttga tgcacatata aaaaatataa gaaaaaaaat atcgttacca tatattaaaa
     7321 ctgtaaaagg aataggatat acacttgaga aggatatttg ataagtggga aaagttaagt
     7381 ataaagtata agttattttc aataacgaca tcattattaa tagcgttagc tttaataata
     7441 tatctaattt tatatttttt attgccatca tattatcatg agtataaaat tgagtctctt
     7501 caagaaagtt taaaatcttt agtagatagt tcaattcatt tcgatacata tactttagaa
     7561 gaaagacttt attatatggc taaagaccaa aatttggcta ttttattaaa agataaccaa
     7621 gggaaaattg tatatggtaa gaatgaagta gttattttaa gatatagtaa gtacatgatt
     7681 aatagtcttg aggatgagta tagaacttct atacctatat atactaaaga tgcaaaagat
     7741 ggtccatata ctttagaact cgttatgcca ttacaaccaa ttgacgaagc aaacgaagtt
     7801 ataagaaaac taatgccata catcatatca atagcaatat tgatagcgat tattggagca
     7861 tatatatatt caattgtaat aacaaagcct cttataaata taatagagag tgagagagaa
     7921 caggaatata gaaggaagga ttttgttgct acaatatcac atgaactaaa aactccaata
     7981 accataataa gtggtcaaat tgaaggtatg atttatagtg taggtaagta taaggatagg
     8041 gatacttatc tgaaaaaatc atatgaatgt acacaagaat taaaagattt agtaaatgaa
     8101 atgatagagg tttcaaaatc agaaatactt gaaaaagatt tgaaattggt atcaataaat
     8161 ataagtgaat tattaaatag acttgtaaag agacaagtat ttttaataga agaaaaacat
     8221 atgaaaacta ttttaaaaat agaagaaaat ttggaaatta aagcagacca agagagaata
     8281 actaaggcaa tcaataatat aataaataat gccataaaat attctcctga aaaatcagag
     8341 cttataatta gactctatga taaaaataaa cgaataagta aaaagacttc taatcaaaga
     8401 gtggttttag aaattgaaaa tactggtgta actatcgaga aacgttattt agaagagata
     8461 tttaatccat tttatagaat agaaaaatcc cgttcaagaa aaactggagg aagtggactt
     8521 ggactttata tagtcagtca aatttttaaa agtcatgggt ttgattatag cataaaaaac
     8581 aaagaaaact ctgtagtatt cacagtagaa tttaaaaatt aattacttat tataaagaat
     8641 atataacttt caataatagt tcattttaaa atcacaaaaa attcataatg aaactgtatt
     8701 atataaatat aaagaagctt aaaaccataa aatacaccaa ctataatgat gagatttcct
     8761 tacatcctcc atataagtca tcattatagt aacccaacaa ttaagttact atgaatttaa
     8821 aaaataacac cgagcatata atctatgctt agtgttattt ttattttgaa ataaaaaata
     8881 tttaatttat tgaaatgtat agatttagac agtttaaact atatttaaga cttaaaaatt
     8941 a
//
LOCUS       DAEPOZ010000149         8870 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.149, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000149 DAEPOZ010000000
VERSION     DAEPOZ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8870
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.149"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            422..799
                     /locus_tag="KR440_002598"
     CDS             422..799
                     /locus_tag="KR440_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH0699635.1"
                     /translation="MKEEILIEKLLRAELIVMEYLCKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLVAKRYLARYIINFQYHYKIIIDNKEYYTFKKKVLKSSKSIKIMRSLT
                     TTHKNVSKEKLDLLDEYYRNLKE"
     gene            904..1279
                     /locus_tag="KR440_002599"
                     /pseudo
     CDS             904..1279
                     /locus_tag="KR440_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     gene            1891..2583
                     /locus_tag="KR440_002600"
     CDS             1891..2583
                     /locus_tag="KR440_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0699636.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            2779..4839
                     /locus_tag="KR440_002601"
     CDS             2779..4839
                     /locus_tag="KR440_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBH0699637.1"
                     /translation="MEKYRALKLVSLIIISISFPLTILLATIGLAEPKYNIQKTSTKS
                     GIFNISNDFYDSNIFDEGTVESLLSNFKISVDSQLTSEEKSKLQTKSKSIDLSSNDSE
                     YDKIMINKQASEEYLSYLKNIKVYVINTYTGVFYTNTSYGNLDDFKQKTKSYCNIEII
                     NNVGKSSYIKTINGEKFELNNLSMELNGVDEAFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVACLSLAIVCLLSIILYSRIKGEVLKRNNVFLLIYSKIPMEFNVILVF
                     ISIAILTMYALAVSYHIDVIVLVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMVISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYIKVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(5045..5368)
                     /locus_tag="KR440_002602"
     CDS             complement(5045..5368)
                     /locus_tag="KR440_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBH0699638.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(5361..6062)
                     /locus_tag="KR440_002603"
     CDS             complement(5361..6062)
                     /locus_tag="KR440_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBH0699639.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(6340..7725)
                     /locus_tag="KR440_002604"
     CDS             complement(6340..7725)
                     /locus_tag="KR440_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBH0699640.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(7833..>8870)
                     /locus_tag="KR440_002605"
     CDS             complement(7833..>8870)
                     /locus_tag="KR440_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBH0699641.1"
                     /translation="YKHNLDNYIPYDQGITLDIFMYIWILGIIAISFYYLRTNFKFYN
                     YIENFKKEILEIDITSILDEQAKELSINKKLQIYKVKGIYSPAIIGLTRCKIIIPEKK
                     YNNKELRFIFKHELIHYKRKDNLFKLLISIICIIHWFNPILYLLKKYFHELCELSCDE
                     IVIDKCTTSEIKEYAYIILNTIKYQKTLKYSSFVSQFLNKKNIILKKRLNSMFNQDKK
                     KINLGLKFLLTFIIIGSIFSINSNFKQNEDVEYTIAKINYNKNGETYGTHIIDSNGNS
                     IEPELIQTIGRGKKVGYVKKRDLYDCDNQPKTPEEAVLYTRKRNFNIFKKTIPLYDKE
                     GIKIIGRFEIN"
BASE COUNT         3512 a          962 c         1231 g         3165 t
ORIGIN      
        1 ttatatgaat tatcagtata ataagtcaaa atatgtatac atgaatacat atttttaaaa
       61 gatatatatt attacatacc taaaaaactt tcataaaaac aaaaataaaa tatgctttta
      121 gtaacttgtt aacataactt ataatttatt ttataagcat tagcctatat agttttgtgc
      181 tactaaattt ctttgattct aacgatttag tgctattcta caactaaaat aatacaatag
      241 agctatttat tgtttggtag cattgtttgc aaattaaaca aatatatata tatgaatatt
      301 attttacgtt taaaaaaaca gactaaatat gtagaatatt tggtaaaact tacctctagt
      361 ttttgtctta aaaagctcct attattaatt taagttagaa gaattaagga agtgacataa
      421 attgaaagaa gaaatattaa tagaaaaatt attaagagca gaattaatag taatggagta
      481 tttatgtaaa aaagacactc taatgcctaa aaaagaaatt gtaaaagaaa taaaacagat
      541 ttatggatgg cataaaagta caataaaaat actactaaaa aggctagttg ctaaaaggta
      601 tttggctaga tatattataa attttcaata tcattataaa ataataatag ataataaaga
      661 atattatact tttaagaaaa aggtactaaa atctagtaaa agcataaaaa taatgcgttc
      721 acttaccact acacataaaa atgtaagtaa agagaagtta gacctattag atgagtatta
      781 tagaaattta aaagaatgat atagtaaaaa tcgaatgtaa ttatttacct taattctttt
      841 gtttgtaaaa ctttattgat atagaattta acatatacat aaattggtaa agggatgtat
      901 actatgaaaa ttagtaaatt accagaagcg gaattaaaag ttatgagata tatttgggaa
      961 tcagaaaagg tattaatatc aagagaaatc accaagtcta tggaacaaaa atatgaatgg
     1021 aaagatacaa ctatatttac agtattaaaa agattactaa agaagagagt ttttagaaac
     1081 ggagaaaata gataagcgta cacactataa tgtattagta aaagaaaaat aatatcagag
     1141 attttaaaca aaagaatttt taaagaatac acataaaaat tcaatattaa gtttaatgtc
     1201 agcactatgt agtaaggatg atgaagtaga tggtgatatt ttaaatttat tagaagaata
     1261 ttttaagaat ttaaaataag aaaatgacag ctagatacat aataaataaa atgaaggtat
     1321 gaaattataa ctttttctac attcattttg tttattattc tgaacatgtt aaaaaaatga
     1381 aataagtttg tattttattt atataaattt taatagaata tgtacagtat atagtagata
     1441 gaatgtataa aaatagaatt taaataagta gctattattg cagtgaaata aatgtttcta
     1501 agtatatttg ggtggaagaa tggtggaaga aaattagtga aatgtaattc ttccaccatt
     1561 cttccacccc aaaaataaaa actgttcaat aagaattaat atacattaat tcagctgaat
     1621 ccaatcaaaa tacttaaata aaagtaatct gaaaataaca taattcaaat caatttattc
     1681 aaataaatct caatttatct taaatcttaa gaaatattta agaattagga taatattttc
     1741 ttaagattta tattttatta tatagttata agggaaagtt acaaaaatgt aagccaaagt
     1801 aatataacaa tttagtcatt gttgagaaag taagtgaaag atattgtata atatagataa
     1861 gtattattat acaatagagg aagtgaaaat atgtacaata tattagtagt agacgatgat
     1921 aaagaaattg ttgattcaat agaaatatat cttaaatcag aaggatttaa aatatataaa
     1981 gcatatgatg gaatgaaagc tttggaaata ataacagaga atgacattca tctaatacta
     2041 atggatataa tgatgcctaa attagatgga atcaaagcaa ctgtaaaaat acgagaagaa
     2101 agaaatattc caataatttt aatttcagca aaaagtgaag atacagataa aataatagga
     2161 ttaaatattg gagctgatga ttatataaca aagccattta atctattaga gcttatagct
     2221 agggttaaat caaacttaag acgatatgta actttaggta attatgaaga tacatgtaaa
     2281 gaaattttaa aaagtggagc tttggagtta aatacactaa caaaagaagt taaaattgat
     2341 ggtgaaaaca taaaaatgac cccaattgag tataaaataa ttgaacttct tttagaaaat
     2401 aaaggacgag ttttctcaat agatgagata tacgaaaaag tatggaatga ggaaagtttt
     2461 aattcagaaa atacagtagc agtacatatc agacgaataa gagaaaagat tgaaataaat
     2521 ccaaaagaac caagattttt aaaggtggtg tggggagttg gttataagat tgaaaaattt
     2581 tagtggaaaa attataaaag aaaagagttt aatatttaat tgcattttga ttttagtcat
     2641 ttttacattt acacaaatgt taggaatttt aatatgtttt aattttttct ctgagagttt
     2701 aatttactct atggggatta tgcttttctt aaactttatt ttatataaaa tactgacaat
     2761 gtagatgagg tgggcaactt ggaaaaatat agagcattaa aattagtttc attaatcata
     2821 atatctatct catttccatt aactatattg ttggctacaa taggacttgc tgagcctaaa
     2881 tacaatatac aaaaaacttc tacaaagagt gggattttta atatatctaa tgatttttat
     2941 gatagtaata tatttgatga aggaacagtg gaaagcttat tatctaattt taagatatct
     3001 gtggatagtc agttaactag tgaagaaaaa agtaaactcc aaacaaaaag taaaagtata
     3061 gatttaagta gtaatgattc tgagtatgat aaaatcatga ttaataaaca agcttctgaa
     3121 gaatatttaa gttatctaaa aaatataaaa gtgtatgtta ttaatacata tacaggagta
     3181 ttttatacaa atacatcata tggtaattta gatgatttta aacaaaaaac taagagctat
     3241 tgtaatatag agataataaa taatgtagga aagagctcgt atattaaaac aataaatgga
     3301 gaaaaatttg aactgaataa tctgagtatg gaactaaatg gagtagatga agcctttgag
     3361 gcatatgtct cttttccaga agagccaact atagaagatg gaatagtata cactaatttt
     3421 caaatattta aacaagctac agaaaaagta agattatttt ttgtagcttg tttaagttta
     3481 gcaattgtgt gtttattatc tatcatttta tatagcagaa taaaaggtga agttttaaaa
     3541 agaaataatg tatttttact aatatacagt aagataccta tggaatttaa tgtaatacta
     3601 gtatttattt caattgcaat tttaactatg tatgctcttg ctgtatctta tcatattgat
     3661 gtaattgtat tagtttcttt atctttactg atagtatcaa ataatatatt ttttataaat
     3721 gtgcaattga gttatttaga aaataaaatg tgtatattta agacaagtat tatagtaaga
     3781 gtgattttat ggttaataaa aactgtaagg gaggtcagta acgctataaa gaaagtatct
     3841 ttagcaagga aagttattgt tatagtgata atatctttag ctttaatcat ggtaattagc
     3901 actcgaagta taggtatggg attactgttt tctatatgta tttttttagg atttacaata
     3961 tatataacta aaagactttc atatcttagt tacgtaatag aaggtacaga aagaataaaa
     4021 aagggagaat tagattataa gataaaaatt actgggaatg ataattttac gtctttagct
     4081 gaaaatataa ataatattgg agaaggtctt gatagagcta tagaagagca agtaaaaagt
     4141 gaaagaatga agtctgatct tataacaaat gtatctcatg atttgaaaac tccacttact
     4201 tctataataa attatgtaga tttaattaaa aaagaagaat caatacaacc agagtatata
     4261 aatgattata taaaggtact tgagtcaaag tcaaaaagac taaaattatt gatagaagat
     4321 ttatttgaag cgagtagagt aagtagtggc aatatagaat tacaaattag caagattgat
     4381 ttagtgcaac tattaagaca aagcataggt gaacttgaag aaaaattatc taaaaataat
     4441 ttatatttaa aattaaatgt tccaaatgat aaggtgtata tatgggcaga tggaagaaga
     4501 atgtatagag tatttgaaaa tcttttatct aatatagcaa aatattcctt agaagaaact
     4561 agagtctata ttgatgtata tgactatggt gaaaatgtaa aggtaactat gaaaaatata
     4621 tcttcttatg aattaaattt tgacccaagt gagattatgg aaagatttaa aagagctgat
     4681 gaatcaagga atactgaagg aagtggatta gggcttgcta ttgcaagaga tttagtagct
     4741 ttacaaggtg gaaaatttta tatagaaata gatggagatt tatttaaagc aaatttagag
     4801 tttcaaaaat atgaagaaga aataacaaat atggaatgag atagtattta agtatatata
     4861 ttttttttac tgtcgaaagc ttttattata atatattaga atatttacag tagagaatga
     4921 agagtaaaaa cagagtaact ttaagtcact ctgtttttta tttaaaatca ataatataac
     4981 aacttaaatt aaaattgtac acatagtttt aaaataactt atacaataaa acattatcca
     5041 aatatcacaa gataattcca aataaatata tagatatcac tcctgtaata atagaaatac
     5101 caatagattt aaacttatat gcaacaagaa gtacaatcat aaaagatata attttagggt
     5161 tagctataga taaatataga ctatctccat taaaaaatac atctttagca gttaaggaag
     5221 taagtattgc tacaggtata aatttcatga tttttttcaa aaaatcaggc atctcttttt
     5281 ttgaaaaaat taacatagga agcactcttg gtagatatgt aacaatgtac attccaatta
     5341 taataaaaaa tatataatta ttattcataa gattcctcct ttttacagta agattcttct
     5401 ataaagaaac caattaaaga tgctaaaatt gatgcaacta ttattactaa attgcttttg
     5461 aataatgcat ataaaataac agaaataatg attgatgata taaatactac aatataaatc
     5521 ttatttttta tttgagaaac tattaaagct ataaacatag aagtcaatat gaagccagaa
     5581 atagaactgt ctatagatat actttctcct aaaaatgctc caataaaatt agctaaaact
     5641 gatgaaatta agcagaagaa attaagataa atagcatttc tatcagacca atttccagca
     5701 gtaaattttt cataattaac tgcaaaagtt tcatctgtta tagtatgaca aaatggcaat
     5761 ataaattttg gacttttatt ttttatgtac atgttcaaat tagaactcat tagaaaatgt
     5821 cttaagttaa ctataaatgt agtcaaaaca atagatgtta cacttgcacc agtagaaatc
     5881 attgaagctg caatgaattg agaacttcct ccaaatacta aagttaccat tagtgctatt
     5941 tgaaaaggtg aaagaccaga tttttgtaat agtattcctc ctactatacc tataggtata
     6001 taacctaaga atataggaat tgaagcaata aagctttctt ttaattgttg agttctttcc
     6061 acaataaatc actccttttg tattaaatgt attaaagtat tagtaaaaca gaaaaataga
     6121 ttaattgtat aattgcaatt ataattaaaa ataaatttgg attaatatat aaaatactat
     6181 acaacaaata aataatctaa aacggttatt aaattttatc tatttgttat tatgatgtaa
     6241 taatacatat taatctctat aagttaatta ctatctggtg taattttatt ttaacaacaa
     6301 aaaaaaatac acacaattaa aatattgtgt gcaatacaat tattttttct cttctaatag
     6361 tctttttaat acgctcagac cagctttaag ttcttctgtg ctttctgtag cacatacagc
     6421 tattcttatt gcagaaattg gtttttcttt tccaacagca aaacgttcag aagcatagac
     6481 ctgtacacct tctttaagtg cttgtatctc aaatttttct gccattattc catcaggtag
     6541 aatcaaccat ctaaatatac attcttcatt tcccaaaaga ttatatccag ataaatattg
     6601 attaataatc ttgtttcttc ttcttgcaga tttacggcgt gcattaatta atttatcggc
     6661 ttcacctgat gtaatcatgc gataagcaat ttctgttaaa aaataagatt gagatagatt
     6721 aatgttgtat aatgcgcttg ataatgcctc tcgatactgt ttaggtgtag agatatatgc
     6781 tagccgtaaa ctaggtgcaa taatttttga taaactcgta atataaattg tttgattggg
     6841 cagataggaa gccactggat ttaaatgtgt ttcattaagt aaactatgaa tagcatcctc
     6901 aataacaatt aaattgtatt tagatgcaat atttgcaatc atttttcgtc cattttgtga
     6961 cattatatgt gtagttggat tttgataatc tggtattatg tatagacctt taatattttc
     7021 atttttacaa gcatataata gtccttcttc actaatctca ttatcttctt gtttaattgg
     7081 aattagttga atcccaagca ttttagctgc tgtctttatt ccaggataag ttaatgggtc
     7141 tacacctatt ctatctccat gttgaaacaa accagctaaa atagcaacaa ttgcattttg
     7201 tccaccactt gcaggaagta agctatctga tgtagtttca agcccagctt tataaattaa
     7261 tttggcaata gcttcttttt gccatttagc accatctctt tgtccatatt ggaatatagt
     7321 tttaaaatct atttccttaa ttatatgttt aaacaaagta ttcatttctt ctagtgtgaa
     7381 atctggattc attgtgccca tttcaataaa tgtaatttta ttattactgg acatcaagaa
     7441 tagatttgaa cgcgtatcat aagatacaaa agttccactt cctgtaactc cacttattag
     7501 accttttttt tcacaaattt taaaagctct agagatagta ctcacattaa catctaagaa
     7561 atctgccaat tcacgttggg gtggtaattt agtacctggt aataaaaatc cattgttgat
     7621 atcttgttct agttgttctg caagcgcctt atataatatt tgcccttttg aaggtctctt
     7681 tggtttccag ttcataggat aattttcaaa agaattaatt ggcataagtt ttcacctcac
     7741 attattaatt tgattttttc tttatctaaa acaataaatt ctaaagatac aaataaaaac
     7801 tgataggaaa taattctatc agtttttgtc ttttaattga tttcaaatct tcctataatt
     7861 tttatacctt ctttatcata aagagggatt gtttttttaa atatattaaa atttcttttt
     7921 cttgtatata aaacagcttc ttctggtgtc tttggttgat tatcacaatc gtacaaatct
     7981 ctttttttta cgtagccgac ttttttacct ctacctattg tttgtataag ttctggttca
     8041 atggaatttc catttgagtc tattatatgt gtaccatagg tttctccatt tttattataa
     8101 ttaatcttag caatagtata ttctacatct tcattttgtt taaaattgga gttgatagaa
     8161 aatattgacc ctattattat aaaggttaat aaaaacttaa gacctaaatt aattttcttt
     8221 ttatcttgat taaacataga atttaacctc ttttttaaaa taatattttt tttatttaga
     8281 aactgagata cgaatgatga atacttcaaa gttttttgat actttattgt atttaatatt
     8341 atatatgcat actctttaat ttcactcgtg gtacatttgt caatcacaat ttcatcacaa
     8401 gataattcac ataattcatg aaaatatttt tttaataagt ataaaattgg attgaaccaa
     8461 tgtatgatac atattatact aattagaagc ttaaataagt tatcttttct tttataatga
     8521 attaattcat gtttaaagat aaatcttaac tctttattat tgtacttttt ttctggaatt
     8581 attattttac agcgtgttaa acctataatg gcaggggaat aaataccttt aaccttatat
     8641 atctgaagtt ttttatttat acttagttct tttgcttgtt catctaagat actagtaata
     8701 tctatttcta aaatttcttt tttaaaattt tctatgtaat tataaaattt aaaatttgtt
     8761 cttaaatagt aaaaagagat tgctataatt cctaatatcc agatgtacat aaatatatct
     8821 aaagtaatgc cctggtcata tggtatataa ttatcgagat tatgtttata
//
LOCUS       DAEPOZ010000150         8865 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.150, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000150 DAEPOZ010000000
VERSION     DAEPOZ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8865
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.150"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(319..1149)
                     /locus_tag="KR440_002606"
     CDS             complement(319..1149)
                     /locus_tag="KR440_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0699642.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKNACNIDANTITLKEHEEEYLVKTPITYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKAYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKI
                     "
     gene            1222..2949
                     /locus_tag="KR440_002607"
     CDS             1222..2949
                     /locus_tag="KR440_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0699643.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLIMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFVKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNREHFEKKRF
                     DRTAIDYSETAISINKVFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALI
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGRKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDVKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEIKHAASIAQADE
                     FISDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            2986..4848
                     /locus_tag="KR440_002608"
     CDS             2986..4848
                     /locus_tag="KR440_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0699644.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKVKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRRAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFSSEYDEEVV"
     gene            5181..5870
                     /locus_tag="KR440_002609"
     CDS             5181..5870
                     /locus_tag="KR440_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HBH0699645.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNNCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSIDLFNKDLPTVSVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            5975..6661
                     /locus_tag="KR440_002610"
     CDS             5975..6661
                     /locus_tag="KR440_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HBH0699646.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKYGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            6678..8585
                     /locus_tag="KR440_002611"
     CDS             6678..8585
                     /locus_tag="KR440_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HBH0699647.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSVDLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLTSVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETHKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDKKEVNAELTSSVVKT
                     NTDNETENKTTNDSESKNNTDKDKSGNDNNQKLEGPRGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
BASE COUNT         3430 a          973 c         1541 g         2921 t
ORIGIN      
        1 aaggatgctt tgatttgcta tatatggaaa aattaaaaat atattcataa tcaaatttat
       61 aaaattaata tctttccatc ctaacattac atttacttag attaattatt attataaaaa
      121 taaaaataca aataatagat tcatttacat tcttaaatga ttaaagctct ttacccttta
      181 aacatgcata acatatcgtt ataattccag taaataagac taaaatgccc attcctaaaa
      241 ttgcagtttc atcatctatt actccaaatg cttttaaagt aacttatttc gctttgtata
      301 ataataattt gtctcttttt atattttaac ttctgctgag atttgagtta gatattcgtt
      361 agaatctcgc acacttatat catatagaag atattcttca taaacaaaat ccccaatacg
      421 taaattattc tgattaaaaa aatttaataa cttttcataa gccttgtaaa tagtttcata
      481 actaccttta tgataagcag ttacataaag tccctttggt ttgattgata caggatattt
      541 cttgtactct ttattaactt ttgtaaataa atatgaatat gattcaaagt ctttattaat
      601 tatatcttca cctttaatga ttgagccaat cgtaccatag tcactcaatt catagttgtc
      661 cataaagtta atacattcat ataaaaaatt ttcttcctca tctcctaaaa actgttcttt
      721 atatgtaata ggtgttttga ccaagtactc ttcctcatgt tcttttaaag taatagtatt
      781 cgcatctata ttacaggcat ttttagtgaa tgaaatagta gattccatta gagcttgaat
      841 attatttagt ttttcaattt catttttaat atcaatggtt ttcttttcta aaagctcaat
      901 tagtaaatta ggtgttcttt tgtctatata agctttaatt tcttttaaag gcatttttac
      961 ttccctaagt gaagaaatta taccaaaaat gctaagttga tggtatgagt agtatctata
     1021 tccattttct ttctttaatt ctggtgagaa aagacctata tcatcataat ggaaaagtgt
     1081 ttttttattg attccacaaa tctttgcaaa ttctccagtt gtaaaataat tttttgaaac
     1141 ttgactcata attataattc ctccttgact atcctgttac agtatagttt atcatagtat
     1201 tttgtaaagg agtgtgatta tatgaaaaaa ttaattcatt ttttaaaacc atatagagtt
     1261 ttaattgttg tagttttgat atttacgttt ttacaaacac ttggaacttt gtatatacca
     1321 acacttacag caaatatagt aaacaatggt gtagtaaagg gagatataga ttatattgta
     1381 aaaacaggtt taataatgat gatagtagct ggtataacag ctttatcagc tgtgttagtt
     1441 tgtaaagtat cagcaaatct ttcatctgga ttttgtagag atattagaga agcagtattt
     1501 gtaaaatcac aagatttatc aataaatgat tttaataata taggtacagc atcaatgatt
     1561 actaggagta ctagtgatat tactttaata ggtcagtctg tgtttatgtt tattcaattg
     1621 gtattaccag caccaattat aactgtttct ggtctattct tagcatattc tatagataaa
     1681 gcaatgacaa ttattattgt tgtagtaatg tttttattca tgctttcagc atttttagtt
     1741 ggtaaaaagc ttataaagtt gtttaaaatg atgcaaataa agatggacaa tatgaatagg
     1801 gtattacgtg aggtagtaac tggagtaaga gttataagag cttttaatag ggagcatttt
     1861 gaaaaaaaga gatttgatag aactgcaata gattattctg aaacagcaat ttcaataaat
     1921 aaagtatttg cagtacttat gcctattgta atgttaataa tgaatttagg tatagtaagt
     1981 attatttggt ttggtgggat gagagtcagt aatggaaata tggagatagg tcatataatg
     2041 gcacttattg aatattgtat cttaatttta ttctatttaa tcatgggagt aatggtattt
     2101 atgtacatac ctagagcagg tgcatgtgca gatagagtaa atcagatatt agatatagaa
     2161 cctgaaattg tagatggaaa tggtcgtaaa gatactgtct cagagagggg acatttagta
     2221 tttaaaaatg taacattttc atatgcacaa tcagaagaac ctgtgttaaa taatataaca
     2281 tttgaagcaa aatctggaga agtaactgca attataggaa gcactggctc aggcaaatct
     2341 actattgcaa atataatacc acgttttttt gaaattcaat ctggagaaat atctataaat
     2401 ggtcaggatg ttaagaagat tcctcaaaaa gaacttagag ataagattgg atttgtgcca
     2461 caaaaagctt ttttatttag tggaacaata gaagaaaata taaggtatgg aaaagaagat
     2521 gcaagtattg aagaaataaa gcatgctgca agtatagctc aagctgatga atttatttct
     2581 gatatggaag acaagtatga ctcatttgta gcacaaggtg gaaataattt gtcaggtggt
     2641 caaaaacaga gaatatccat tgcaagagcg ttagttagaa agcctgaagt atatgtcttt
     2701 gatgatagtt tttctgcatt agattttaaa acagataaaa gacttagaaa agcgttgaaa
     2761 aatgaaatca aagattcatc agctataatt atagcacaac gtataagtac aatcatggat
     2821 gctaatcaga ttatagtttt aaatgatgga aaaattgtag gtattggaaa acataaagat
     2881 ttacttgaaa attgtgaagt ttataagcaa attgcggatt cacaacttag taaagaagaa
     2941 cttgcataaa atgaagaatt aatctgatat ttggaggtgt atgatatgag tgaaaaaaga
     3001 atagatgaac aagatataga agtacttagt tttgaagatg gcatgtcagc agaaaaagtt
     3061 aaggatgcaa aaaaaactac taagagatta ttaaaatata tggcaaaaca aaagtttaaa
     3121 atattaatta tttttatttc agtattgata agttcagcgc ttacagtttt agcacctatg
     3181 gttatgggaa aagctattga ccaactattt aatggaataa aaactgcagt ccaaacaggt
     3241 acaaaattca gtgtgaattt tagtactatg ggggggatag tatcaatact tctagggttg
     3301 tacttaatta gtgcagtatt tatatatatt caacagtata ttatgtcagg agttgcacaa
     3361 aatctagttc ttagcatgag aaaagattta agtgataaac tcaataaact tcctcttaag
     3421 tactatgatt ctcataaaaa gggggaaaca cttagtatag taacaaatga tttagaaaaa
     3481 gtagcagata gtttacaaga aggattaatg caacttatta cagcagtagt tacagtagtt
     3541 ggttctattg taatgatgat aagtataagt gtaccactta caatagtatc agctataaca
     3601 ttacttgttt cattgggtat tactgttgta attgcaagaa aatcacaaga aagattctct
     3661 gaaaatcaaa aggcactagg agagttaaat agcaatatag aagagatatt tactggtcaa
     3721 atagtggtta aagcatttag taaagaaaag gatactataa gaaactttaa aatattaaat
     3781 caaaatcttt ataatgcaag tagaagagca cagttttcgt catatgcaat atctccaatt
     3841 attagattta taaacctaat tggctatgtt attatagcag ttgtaggtgg gatatttgca
     3901 tcaactggag caatgactct tggtagtata caagcgttta ttcaatatgt aaatcaagca
     3961 tcagagccta ctacagaaat atcttatatt gtaaatatgc ttcaagcagc aatagcatct
     4021 gcagagagag tttttacagt tatggatgag gtggaagaga taaaggatga agaatcttca
     4081 aaggttatta gcatgcctaa aggtaaagta caatttgagc atgttaaatt tggatatagt
     4141 gatgatttta ttcttatgaa agatattaat atcaatctaa atgcaggaga caagattgca
     4201 attgttggac ctacaggagc tggaaagaca actcttgtaa atttacttat gagattttat
     4261 gagatacaag gtggaagaat tactattgat ggtgttaata taaaagactt aaagcgtgga
     4321 gaacttagaa ctatgtttgg tatggttctt caagatacat ggctgtttaa tggaagtata
     4381 aaagaaaata ttgcatatag taagtcagat gccacaatgg atgaaattgt aagagcagca
     4441 aaatcagcaa gagtagacca ttttataagg actttaccac aagggtatga cactatattg
     4501 actgaggatg cttctaatct atctcaaggt caaaagcaac ttctaacaat tgccagggca
     4561 atactttcag acccatcagt attgattctt gatgaagcaa catctagtgt agatactaga
     4621 acagaggtag aaattcaaaa ggcaatgaat aacttgatga agggaagaac tagttttgtt
     4681 attgcacata gattatcaac tattcgagat gctgatttaa tattagtaat gaaagaaggc
     4741 actattattg aacaaggaag ccataaggaa cttattgaaa agaaaggatt ctatgaagaa
     4801 ctttataata gtcaattttc aagtgaatat gatgaagaag ttgtatgatt atcaagtttt
     4861 aggcaactaa atgattgata aatattaagt taaatatagt aacatgtaag gttatatgga
     4921 ataagagtat ttctctattt catataacct tttttattta tcaaaataag ataaaatttt
     4981 ctttctaaat tagattgaag aaagtattta tcaatataaa agtaaaataa tcattattat
     5041 tagtgaagta aatattgttt gccacatata tgccataaag atgcacttat tttacggaga
     5101 tttaacaaag tagtgtaaag tatactctaa atataaaata aatcaaatag agttaaataa
     5161 attgagaggt gatataaata gtgaataaaa aattgaacta tagatttgaa atgaagcaca
     5221 aaataactga agcagatgtg ttagctttaa aatcaagact ctatccaata atgaagaaag
     5281 atgaaaatgc atctaaagat ggaaaatatc ttattaggag tttatatttt gatactcctg
     5341 aagataaagc acttttagat aaattaaatg gcgttgcaat aagagaaaag tttaggataa
     5401 gattttataa taataactgc tcttatataa ggttagagaa aaaaatcaaa cattacaaca
     5461 tgacatcaaa gctatcaact aatttaacca aaaaagaagt aataaatata ctaaacaatg
     5521 atattgaatt tctaaaagag tcgacaaatt gtttattaag agaattttat ttgaaattaa
     5581 aatgtgaaag attagaggca aaaagcatag ttgattacaa tagagaagct tttatttatc
     5641 cagttggaaa tgtaagagtt acaatcgata gtgatattaa aacttctgta aattcgatag
     5701 atttatttaa taaagatttg ccgactgttt cggtaattga tgagaatatg actgtattgg
     5761 aggttaaata tgatgaattt ataccagatt ttataaagga tttaattcaa attaataaga
     5821 ctacttctac agcagtttca aagtatgctt caagtagatt atatatttaa gaataaaggc
     5881 tatgatttat ataaagatat acataaataa ggttaaaaaa caaataggtt aaaaataaat
     5941 aaaataaaaa ataaataagt taaaaatagg aaggatgatt ttaattatga catttaatga
     6001 tatttttaaa tcaagtttta tagaaaacgt atcaggtttt tcatttgtgg acagtgcttt
     6061 agcactaggt tcagcttttt tagtaggttt gtttatatat atggtctata aaaagactta
     6121 tatgggaatt atgtattctc gaccattcaa tgtgtcgctt gtagcactta caatgcttac
     6181 aacttttgta atattggcgg ttacttcaaa tgtagtttta tccttaggta tggttggtgc
     6241 tttatctata gtaagattta gaacagcaat taaagaccca atggatttag tatttctatt
     6301 ttggtcattg ggttcaggta tagttttagg agcaggtttg attcccttag ctgtcatggg
     6361 ttcaataatt atgggattga ttctaatatt cttttcaaat aaaacaatat ctgaaactcc
     6421 atatatattg atggtaaatt gtaataatga agattcagaa gatatagcaa ctgataaaat
     6481 taaaaaagta tttactaaat atcaaataaa atcaaaaagt gttacaccag aaaaaggaat
     6541 agaattagtg tttgaagtaa gaatgaaata tggagagaca agtcttataa atgacttgag
     6601 tcaagtagat ggagttacta atgcagttct tgtaagctat aatggtgact atgtagcata
     6661 gagaggagta atcttttatg aaagataaaa aatttaccct tcttatctct attatgatta
     6721 tatttttatg tgctgtagtt ggagtttata gtacatctag caacaaaagt gttgatttat
     6781 atagtgatgt atatattgaa aaatatttta acagagacaa ggttatggaa gttaatatag
     6841 agatagatga aagtgacttg aaggatatga atgaaaatgc tataaaagaa gaatttaagg
     6901 ttgcaaaagt aactgtagat ggagatacat atggaaacgt aggtataaga actaaaggaa
     6961 attcaagtct tacatctgta gcaaatagtg atagtgatag atacagctat aagattaatt
     7021 ttgataagta taatactagt caaagtatgg aagggcttac tcaattaaat cttaataact
     7081 gttactctga cccatcttat atgagagagt ttttaacata tagtatttgc gaggaaatgg
     7141 gattagcgac tccagaattt gcatatgcta aagtctctat aaatggcgaa tatcatggtt
     7201 tgtatttggc agtagaagga ttaaaagagt cttatcttga aaataatttt ggtaatgtaa
     7261 ctggagactt atataagtca gatgaaggaa gctcgttgca atataaagga gatgacccag
     7321 aaagttactc aaacttaatc gttgaaagtg ataaaaagac agctgattgg tctaaaatta
     7381 caaaactatt aaaatctttg gatacaggtg aagatattga aaaatatctt gatgtagatt
     7441 ctgtccttaa aaatatagca ataaatacag ctttattaaa ccttgatagc tatcaaggca
     7501 gttttgccca taactattat ttatatgagc aagatggagt attttctatg ttaccatggg
     7561 attttaatat gtcatttggt ggatttagtg gttttggtgg aggtagtcaa tctatagcaa
     7621 ttgatgaacc tacgacaggt aatttagaag acagacctct catatcctcg ttattaaaaa
     7681 atgagacaca caaaacaaaa taccataaat atctggaaga gatagtaaca aaatacctag
     7741 attcagacta tttagagaat atgacaacaa aattgcatga catgatagca tcatatgtaa
     7801 aagaagaccc aacagcattt tatacttatg aagaatttga aaaaaatata acatcttcaa
     7861 ttgaagattc tagtgataat aagggatttg gtaataaagg gtttgacaac aataactcta
     7921 ataacagtga ttctaataat aattctaata gtgaaaataa gcgctctgga aatcaaagtg
     7981 ataaaaaaga agttaatgct gaattaacat caagcgtagt caaaactaat acagataatg
     8041 aaactgaaaa taaaactaca aatgatagcg aaagtaagaa taatacagat aaagataaaa
     8101 gtggaaatga taataatcaa aagctagaag gtcctagggg taaaggaggt aagtcaatac
     8161 caggggtttt ggaagttgca gaagatatga gtaaaactat aaaatctcaa ttaagtggag
     8221 aaacttcttc gacaaagcaa aactctggtg atgaaagttc aagtggaatt aaaggtagtg
     8281 aaaagtttga tgaggatatg agtggtatgc cagaaccacc tgagggaatg gatggtaaaa
     8341 tgccaccagg aatgggtaat atggataagg gagatatgaa tggtaaaaat ggcaatatga
     8401 atatggatag aaatcaagat aatccaagag aagctggagg ttttggcaat agaggaggag
     8461 gctctgtgag taaaacaaca acatacttca aattaatttt aggtggagct tcaatgataa
     8521 taatgtcgat tatgttagta ggtgtatcaa gggtaaagag aagaagattt ataaagtcaa
     8581 aataagatta aaattgaata taaataattt taatactgtt aagtctaaat atttacttag
     8641 cagtattttt ttagaaaata atgtgtttga ttaataatat aaggtaataa attgataaaa
     8701 attaaataca gcatattaat aatacataat aaagtaataa aaaaactgtt taagtataat
     8761 aacttatatt tagacagttt ttttcattta taaataatat aaatgaaatt gaaaacattt
     8821 gaaggataga aattggttgg ataaatgtat aaattacttc accaa
//
LOCUS       DAEPOZ010000151         8863 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.151, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000151 DAEPOZ010000000
VERSION     DAEPOZ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8863
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.151"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..259)
                     /locus_tag="KR440_002612"
     CDS             complement(<1..259)
                     /locus_tag="KR440_002612"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HBH0699648.1"
                     /translation="MVFETFDKVIKLHPNAKPVFHSDRGFQYTSKVFESKLLAQGMIQ
                     SMSRAGRCIDNAPMEGIWGILKVEMYYLRKFDTYEQLVKIIE"
     gene            355..848
                     /locus_tag="KR440_002613"
                     /pseudo
     CDS             355..848
                     /locus_tag="KR440_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429361.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            1390..2745
                     /locus_tag="KR440_002614"
     CDS             1390..2745
                     /locus_tag="KR440_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0699649.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATVLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(2863..3531)
                     /locus_tag="KR440_002615"
     CDS             complement(2863..3531)
                     /locus_tag="KR440_002615"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HBH0699650.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREDLVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(3598..4026)
                     /locus_tag="KR440_002616"
     CDS             complement(3598..4026)
                     /locus_tag="KR440_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBH0699651.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            4133..4726
                     /locus_tag="KR440_002617"
     CDS             4133..4726
                     /locus_tag="KR440_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBH0699652.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENATKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVL
                     IIACILLFVFPQKIQSIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVSIMSFFIILFGLVLFFSGNTARKY"
     gene            4846..5781
                     /locus_tag="KR440_002618"
     CDS             4846..5781
                     /locus_tag="KR440_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBH0699653.1"
                     /translation="MDKFKYAFDDKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKARNSYQGCKFV"
     gene            complement(6052..6996)
                     /locus_tag="KR440_002619"
     CDS             complement(6052..6996)
                     /locus_tag="KR440_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBH0699654.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKISKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKVCYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
     gene            7312..8742
                     /locus_tag="KR440_002620"
     CDS             7312..8742
                     /locus_tag="KR440_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0699655.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGNLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNADLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
BASE COUNT         3271 a         1262 c         1122 g         3208 t
ORIGIN      
        1 tctctattat tttaactaat tgttcatatg tatcaaattt ccttaaatag tacatttcca
       61 cctttaaaat tccccaaatt ccttccatag gagcattgtc aatacaacga ccagctcttg
      121 acatactttg tatcatacct tgtgctaaaa gtttcgattc aaacacctta ctagtatact
      181 gaaaacctct atcactatga aaaacaggtt ttgcatttgg atgtagtttt attactttat
      241 caaaagtttc gaataccaag ttattattat tcgattttcc aataacatat gaaattaata
      301 gatataacgc cttctatttt acagtcttat ataaataaca tatacaatga gcacatgtta
      361 aattcaataa aaaaccaagt cgattttata aaatcagtat tgaaagaatc ttatagatta
      421 aaagaaatat cagaaaatat ttgtgatttt gttacaaccc caatcaaaaa aaatagttca
      481 acttctaagt tttatacaaa gcaagaaact caattattat tagaaaaatc aaaaaatatt
      541 cctataggta taccaatttt tttgatacta actcttggat tacgttttgg tgaagctgta
      601 tcacttatat ggtcagatgt agaccttgat aaaaaatctc acatgtaaat caaacattag
      661 tttatgtata caataaaata atcttcaaag accctaaaat ccctagatcc aaaagaaaat
      721 tatttgctcc tgatgaactt ataagtttgc ttaaagggca aaaattaaaa caaaataagt
      781 taaaattaca gggtatattg aaaaatgaat ttgatttgat atgcttaaat aaaagattca
      841 acccgtgagt tcaaccaact tttgttgttc attttaaaaa actattaaga gataataatc
      901 ctagagatat aaaagtacat gaattaatac atgcaaatgc gactttgata ttactttcag
      961 gtactaacat aaaagctatt tataaaatat tagtacatgc agatataaaa atagctatga
     1021 ataaatattc gatattctca cttattagat gaaatggata aagaaacatc tgaaaatata
     1081 agcaaaatac tattcaaata atgttcagtt ttatgtcaat taaatgtcac tcaaagtgct
     1141 ttttttccat ttgtcagtta aatgtcactt aaagtacctt tttcctattt gtcagttaaa
     1201 tgtcagtaat atataaataa gatatattta taagaccctt aaaatcacta ttatttaata
     1261 aaatttataa ttatatacta tttgctgttg acattggtat tataccatag tttatcattt
     1321 atattagaaa taaaattatt aattaatagt ttcgtaattt tataatagat ttacagggag
     1381 tgataataaa tggatacaaa aatatctata aacgaaaaca tgagtctagg taaaagattt
     1441 tttaaatacc tagcaccatc tgtagttgca atgtgggttt tctcactata tacaatggtt
     1501 gatggaatat ttgtaagtaa aggagttgga gaattggctt tagctgctgt caatatatca
     1561 atgcctttta ttaattttat atttgcagtt tctctattat tttcaactgg tgcatctacc
     1621 ataattgcaa tctatctggg gaaaaaagat attaaatctg ctaacgaagt cttttcattt
     1681 aacttagtat caataattat attatcaata attatattag ctataacttt ttttaattta
     1741 gatagattag cactattttt aggagctaca gaatctacaa ttggtatggt taaagattat
     1801 ttaggaataa taatattttt taacggattc tttatagtgt cttattcttt agaagttata
     1861 ataaaaactg atggctttcc aatattagct actgttggtg taataatatc tgctcttaca
     1921 aacatcatac ttgattactt gtttgtaatt gaatttggat ggggtgtaaa aggtgctggt
     1981 attgcaactg ttttgtctca agtcttttct actattttct tcttaataca ttttttaaga
     2041 aaaaactcta ctttgaattt ttcaaaattt agaattgatt ttaagacact tagaaaaatt
     2101 gtatttatag gttttcctga ttcaactact gaattaagtt gtggtatagt tgttttactt
     2161 tttaacttaa gtcttactaa atatattgga gaaaatgctc ttatttatta tagtgtcata
     2221 aattatataa atacattagt acttatgact atgatgggta taactcaagg tatgcaacca
     2281 cttactagct tttattatgg tgcaggaaat atagataatg taaaaaaatt attaaaaatg
     2341 gggataaaag ccacaataat agcatcagtt gctgtatttg caatttgtat ggctttttca
     2401 ggaccaatag tctctttatt tatacaccca gaagaaacaa tgctattcaa tgaaggagtt
     2461 cgtgtattta aaatattctc tatatctttc ttattggtag gaattaatgt aataatttct
     2521 ggattctttg tatcagtaga aaaaccatca atttctactg ttatatctct tggtagaggg
     2581 cttgtaatag ttgttttaag tctaatttca atgatactaa tatttggtgg acaaggaata
     2641 tggatgacta ctattgtttc tgagtttata tgtcttattt tatcattagt ttttctaaaa
     2701 aagaattttt ctacattaga ctctaatcta aataaggttg cttagatgat aagagtagca
     2761 atatatgaaa atatctaaat aaaaaatacc tattatacaa tcttgcaaat attagacctt
     2821 tgcaaaaatt aggattatat aataggtatt ttatttatgt aattatttta tttctaaata
     2881 tccatagcca tcttttatta aagttccttt tacttcatat tctacaggtt ttttaaatat
     2941 tggaccatct acgacaaatg tatgatactc tccatgttct ccacatgggt ctgcaccagc
     3001 attttttatc ttctccacaa gttctctagt taattttttt cctaaaaatt cctctcctaa
     3061 gtgctttaag tttacttttg ttacaacact acaaaatcca ctgtctataa attcatatac
     3121 taaatcctct ctactctctt gccataatgg taaatctact ttcataccaa ctgcttcaca
     3181 tctatcagta tcccattttc tatgactttc tatatctata tctccatatg cacatatcgt
     3241 tgctcccatg tttttagctt ttattaaagc cctctcaaag ctctcttcat atccttctac
     3301 accacactca gcaactaata aaggtaatcc tatattgcta cttacttgct taagcatatt
     3361 atattctaaa ttatgtgtcc atgactttcc atgttcttca cttatagttg tcaagagtgc
     3421 cacaggctta tatccttttt tcaacattcg atttaatgct aatatactat ccttaccacc
     3481 actaaatgac attacaaatt tttcatcagc caattttaac acctctttca tctaactttg
     3541 ttttattaac ataattatac caaatttttg agttgcatag caaattatat taaattgtta
     3601 attcactata aagaatgtcc ctaatattaa aagtataacc cctatgtatt tgtataaatg
     3661 cattgtttct tgtcccaaaa ttacgctatc aataaaaaat ccaactaaaa cttgggatac
     3721 aagtgttaat ataagtgtat ttgatactcc taaatttgga attgccttta ctgtaaaata
     3781 tattattaaa cctccaaata ctcctcctaa taacaactta taattaaaac catttataga
     3841 tactagacac tttaagtcac ctgtaactat tatacttgcc aaaaaaaata ttgctcctac
     3901 agttaaactt ataaaggtgg ccactaatgc tgttgtactt tttcctagtt ctccatttat
     3961 aaaagcttca agagctgtag aaccacctgc taataatgca aatattattg cccataattt
     4021 tgtcatggac aaacctcctt ctatataatt ctattaatag aagtctatga tacaacctat
     4081 taattaatta ctaagtatag tttaattttt atacactagg ctttatttgg atatgaattt
     4141 ctgttataat tgttctatcg attttggagg tgattttatg tacacaaatt ttaatatcga
     4201 ttttaataac tttaataaaa aagaaaatgc tactaaattt atgttaatgg gagtattgct
     4261 cattatctta ggattattat gccttacatt taagacatta ggtataaaat taatttcttg
     4321 gacttttggt attgcacttt tattctttgc atatttaaat ttaaaaaata ttaatgaact
     4381 aaaaagatat gctactaaag aagaaattaa accctctatt aatatccaat gggttctaat
     4441 aattgcatgt atattacttt ttgtatttcc tcaaaaaata caaagcatat tttcattatt
     4501 tttagggttt tatttaatct ttaatcaact agtagcactt gtaaatagta agaataatcc
     4561 ttattctaaa tttacaactt ggaatatagt taagattcta tttggtatat gccttatatt
     4621 atctccactt tttttatcta gatttatagt atcgataatg tcttttttca taatattatt
     4681 tggactagtt ctatttttct ctggaaatac agcgagaaaa tattaattat tttctcgctg
     4741 tatttttata ttacttaaaa tagaatttgt ttaagttatc tatttgtggt aatatgtttt
     4801 ttgtacaagc tcagcgtaca attttattag aaataggtga tttttttgga taaatttaaa
     4861 tatgcttttg atgataaaag atatcatact tggaattact atcttagaaa tacttttgga
     4921 gaaaaagttt ttaaggtatc tataaatgca ggatttacct gcccaaacat tgatggaagt
     4981 cttggttatg gcggatgtac ctactgtagc aaagaaggct caggtgattt tgctggaaac
     5041 cctaaagata atcttatcag tcaattttat aatataaaag aaatgatgtt aaaaaaatgg
     5101 cctcatgcaa agtatattgg ctactttcaa gcctatacaa atacgtatgc tcctcttgaa
     5161 gttttaaaag aaaaatatga gactatatta gaacttgaag atgtcatagg tctttcaata
     5221 tccacaagac ctgactgttt gccagatgat gttgtagaat acttatctga actaaataaa
     5281 agaaccaatt tatgggttga acttggactt caaacaattc atgattctac atctaaaatt
     5341 ataaatagag gtcatgatta taaaacattt gttgaaggtg ttgaaaaatt aaagtctaaa
     5401 aatataaagg ttgtcgtaca cattataaat ggtcttcctg gtgaagatta taatatgatg
     5461 atggaaacag caaaggcagt tggtaaaatg ggtgtagacg gtataaaaat ccatctattg
     5521 catgttataa aagatactcc tatggaaaag atgttacaaa atggtatgtt aactcttatg
     5581 gaacaagatg aatatatcaa tcttgtctgt gaccaattag aaatacttcc tgaaactatg
     5641 ataatccata gactcactgg tgatggtaag agggatgaac tagtaggtcc tatatggagc
     5701 ctaaaaaaat gggaaatttt aaatcaaatt gatgatactt taaaggctag aaattcttat
     5761 cagggctgta agtttgttta atacaaaata ataactatag aaatggttat ataaatatag
     5821 tcctttctat agttatttag tatttacaaa acaaaatatt aatttgtaca tttatacaat
     5881 atataataaa aaatagatag tctaaactgt atctacttat attttatttg tactgtccac
     5941 tttattctat aaaatttata aatagttatt ttttaatacc aaatataatt tggtataatt
     6001 tttgttggat tctaaaatgt ataccaataa atccttttta agtatataat tctaaatact
     6061 ttcagttttt tcaatctgct ttaacttgtt ggatttataa aatattctta tttcatcata
     6121 agaaagatta agtgctacta ctgcagatat taacatcaac atcactccaa ataacttccc
     6181 taaggaaaaa ctttctccta atatagtgca accaattatt actgaaccaa ctaactctac
     6241 tgatgccaca acacctgcaa ctgacaactc tacaccttta gatatacctg ctgcataaaa
     6301 aatatatgca agtgcagctg gaacaatgcc taacattagc atacaagata aaacatctaa
     6361 atccttagca taatttaaga tttcccaagg tctcgaagaa ggaatcataa atatagctcc
     6421 aaataagaaa ctgtatacta atattgttga acttgaaaat tcctttaaag catttttact
     6481 aatagttggc ataagtgcat atgctattgc tgataatacc ccaagtaaaa gtccaaaagc
     6541 gtttaaacct tgaaaatcca aacgtccacc agtcactgcc ataattgcac caataaaaca
     6601 aagaataagt gaaaataatt tgcttctagt aatattttct ttataacata cttttgaaaa
     6661 tattgctaaa aacagtggtg atgtatatag taaaactgct gcaattgata cacctgcaat
     6721 gtcaattgct ttaaaataac ataaattaaa cattgcctga catataattc ctattattac
     6781 tgaatatata attccttttt tgcttatctt taagatttgt ggtgtcctta tagatgaata
     6841 tacgcctaaa actataaatc caaaaaacaa tcttgtaaat gctacttgtt ctgatgtcag
     6901 tccatttccc attaaaaact ttccaaataa tccaagtgta gcccataata accctgctat
     6961 tgctataaat acataccctt tacttttttc tcccatttta aattcccttc tatttttatt
     7021 tagtcaattt acactcttct ttttaagcaa aaattatgcc atcttaaacc aatatatatc
     7081 gactctaaaa ccctagtctt tccaataata ctaataagtt ttcaattttc ttatatttat
     7141 ttttcgctta ctttaagttg atttagactt attttaattg taaaatgagt tatatataac
     7201 ttatttaaag ttaaaataga ctgaaactta tataaatatc aaattttaag tatattaatc
     7261 aattaaacct attttaatat atcattaaaa atttaggagg aaaatataaa aatgaattta
     7321 gatttggaat tttatcaaaa aatattagaa gcttcccatg atgaaatatg tgtttctgat
     7381 gataaaggaa taataatata ttgtaataaa gcatttgaag aaaattatgg tctaaaaaaa
     7441 gaagatattt taggaaaaaa tgtttctttt ttagaagact ctggatattc aacaaaaagc
     7501 cccataccaa ttgtattaaa aacaaagact aaattttcac tggaacaaga tactcaaaca
     7561 gggaaaaagc ttatcataac tgctacccct atatttgatg aaaatggaaa cttagaattt
     7621 actgttgaaa attgtagaga tataactgaa cttaataata taaaaaataa acttgaagac
     7681 acaaaaaagc aagtaaaaaa atataaatct gaggtagaaa cactatacag aactgcactt
     7741 agaattgaag atactgtaat tatggacgga atagtgatga gaccaatcat aaacacagta
     7801 aatcatgttt ctaaaacgga tgtaagtgta cttttattgg gtgaatctgg aacaggaaaa
     7861 agctcacttg ctagatatat acaccacaac agcaatagat ctaatggccc atttataaca
     7921 ataaattgtg caactatatc tccacaactt cttgaatctg aactttttgg atatacttca
     7981 ggtgctttta caggtgcaag tactaaaggt aaagttgggc tcgtagaatt agctaatgga
     8041 ggaactcttt ttttagatga aataggagat ataccacaaa atctacaggc aaaatttttg
     8101 caattaatac aggatagaac ttttactcca gttgggagct taaaaaataa aaaggttgat
     8161 ataagaatta tatctgctac aaatgctgat ttagtttcaa aagtaaaaga aaaaaagttt
     8221 agagaagatt tatactatag attaaatgtt atagaaataa aattgcctcc tcttagagaa
     8281 agaagggata atctagtgga aattataaaa tattatttta ataggtactc aagtgacttc
     8341 aacctaaata aaactatttc taaagaagct atggatgcta ttgcaaatta tagattccca
     8401 ggcaatataa gagaattaca aaatataatt caaaaaatct tgcttacttg tacagataat
     8461 catataacaa tagatgactt accaaacatt cttacaaaaa atatacatat taccaataat
     8521 ggaaataaaa cacatatatc ccaaattaat aaggttatta tatctgactc aaaatctact
     8581 aattataaaa ataaaaattt tgacacctta attaaagagt atgaaaaaaa tataattttg
     8641 gatgcttatg aaaaatttgg tagttcttat aaagtagcca aacaccttga aatctcacag
     8701 tctaaagcca ataggttaat tagaaagtat actaatactt aattttaaat ttgataaata
     8761 aaaacttaac actatttaca tattgtaaat ttggccattt actgttatta tgaatatggt
     8821 tgaaattatt taaataaata ctgttgactt agtttcatat att
//
LOCUS       DAEPOZ010000152         8771 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.152, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000152 DAEPOZ010000000
VERSION     DAEPOZ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8771
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.152"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..253)
                     /locus_tag="KR440_002621"
     CDS             complement(<1..253)
                     /locus_tag="KR440_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0699656.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMD"
     gene            487..939
                     /locus_tag="KR440_002622"
     CDS             487..939
                     /locus_tag="KR440_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0699657.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGIATNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTSEVKNASDASHLVKYITNKMKEE"
     gene            942..1250
                     /locus_tag="KR440_002623"
     CDS             942..1250
                     /locus_tag="KR440_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBH0699658.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            1364..2470
                     /locus_tag="KR440_002624"
     CDS             1364..2470
                     /locus_tag="KR440_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBH0699659.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAM
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            2545..5223
                     /gene="mngB"
                     /locus_tag="KR440_002625"
     CDS             2545..5223
                     /gene="mngB"
                     /locus_tag="KR440_002625"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBH0699660.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDEYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIGKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISNIENYIDSVKNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTANEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGEILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESSKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPASLEERSIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLLFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNNSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(5540..7873)
                     /locus_tag="KR440_002626"
     CDS             complement(5540..7873)
                     /locus_tag="KR440_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0699661.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQAGAKGGA
                     KNPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKVINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLL
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKTVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(7848..8564)
                     /locus_tag="KR440_002627"
     CDS             complement(7848..8564)
                     /locus_tag="KR440_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0699662.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMRLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVNIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAINTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
BASE COUNT         3273 a         1235 c         1183 g         3080 t
ORIGIN      
        1 aatccataat atcatcagat attaatgcta gatactgttg gtataatgct aaattaagaa
       61 tatctatata atatccttct ccttttttta ttttaataat agcaccattt ttctctaaag
      121 ttccatttat agaagtaata tcatttctta tagttctctc actcactttt aaatgattac
      181 ttaaagtttt acaagtaatt ttatcatcac tttcatttaa taatttaaac aacgtgagca
      241 atctcttgtg cataaatatc cccccttata gaatttcttc taaattatac tatttacact
      301 catataatac aattactcta ataaaattgt attataataa tttctttcca cttctatatg
      361 gaaattagtt attatcataa tataaatttc catgtcgaag tggaaattag ttatatttaa
      421 tttaattagg ataatgatta caatataaat ataagttaag acattacgaa tggagtggaa
      481 aaatatatgg tactacaaga aaaatatata tttatagata aagacttaaa aagcaaagaa
      541 gaagtattaa attttattgc agatagtgct tatatactag gtattgcaac taataaagag
      601 gctcttttaa aagaccttct aaaaagagaa aatgaatttc caacaggaat aaaagatgga
      661 tttgcgatac ctcatacaaa aagtgtaaat gtaaaggaag cttccatact ctatattaaa
      721 acaactaacg agttagagtg gaaaacttta gatgatagta aggttagata tgtatttagt
      781 ttgttagccc cacataaaaa tgaaggaaat atacatttaa aaactctatc taaattagcc
      841 acatgtttaa tggaggatga ttttacaagt gaagttaaaa atgctagtga tgcaagtcat
      901 ttagtaaaat atataactaa taaaatgaag gaggaataat tatgaaaata gtagcaataa
      961 catcatgtat agcaggatta gcacacacac ctatggcagc aaaagcttta gaacaagctg
     1021 catcaaaaat gggtcatgaa atcaaagtag aacaacaagg tgctatggga cgagtaaata
     1081 ctataacaaa agaagaagca caagatgcgg actttatact tatagcatct gaccaaacta
     1141 tatctggaat ggaaagattt gatggtaaaa aagtgataaa agtcaaaata ggtatagcgt
     1201 taaaaaaacc agaagctgtc ttaacaaagt gtatagaagc tatttcttaa taaaaaaatc
     1261 tcactctaat atagaatgag aaaaataaaa aattaaaaaa ttgtctaatg taattataac
     1321 atattatcaa aaaatataac aaacttatat aggaggatta aaaatgagta aaaaaaataa
     1381 atctctttta agtgaactta agggacactt aatgacaggt atctcatata tgttgccact
     1441 tgtaataggc tcatctttag ttgtagctat cccaaagctt acagctttag caatgggtat
     1501 aacaagttta gatgcttata aaaatgtatc tggtttttat catattcttt acttaatgga
     1561 acaagttggt tggactggca taggtctatt aaatacggta ttagcaggat ttatagcatt
     1621 ttcaataggt gagaaaccag ctatgggtgc aggttttatt ggtgggctca tagcaagtaa
     1681 tactaatgca ggattcttag gagcagtaat agcaggtttc tttgctgggt atgtttgtaa
     1741 gtttttaaag aaaaaaatta aaatatctgg gtcagcatca ggaatgatgc cacttataat
     1801 aatgccactt ataacagttg gattaactgg ttttttaatg agtgttgtat tagcaggacc
     1861 tttaggaaac ataaatactt cattaaatgc ttgggtagct gaaatgtgtc aaaatggaac
     1921 aaattcggtg gtattggctt taattttagg cgcaatgata ggattcgatt taggtggacc
     1981 agtaaataaa gcagcttgga tggcaggaaa tgcattattg ttagagggaa tctatctacc
     2041 agcgatattg gtaaactgcg caatagttat acctccacta ggatatggtt tagctacatt
     2101 cataagaaaa tcaaaatttt ccaatgcatt agctgaaaca ggaaaaggca atatagttat
     2161 gggtataatt ggaataacag aaggtgcaat tcctttcact ttaacaaatc cattaaaatt
     2221 agttcctgtt aatatgatag gatgcgcttt gggtgtagga ttatcagctt tgttaggtgt
     2281 acatgctata atgcctccag ttggtggatt atatggattt atatcaatag gttctggatg
     2341 ggcttactta gtaggtgcaa tatttggagc tttagtaata gcaatattat caacaacttt
     2401 agtagacttc aatgaagatg aaactacgga aaatacagaa tttgatgaaa tagagttaga
     2461 tataatgtaa attaaaaact taattttaga taattgagaa ttgagtgtac tctcaattct
     2521 caattaaatt ttgggaggat ttttatgaaa aagacaaaag tacatgtagt tccacacagt
     2581 cattgggata gagaatggta ttttacaaca tcaaggtcaa aaatatattt aatgaaagat
     2641 tttcaagata tattagacat attggaagaa aaagaggact ttaaatactt tactatggat
     2701 gcacaagctt ctctcttaga tgattattta aaatggagac ctcaggatga atatagaata
     2761 agagatttag ttcaaagaag aagattagtt attggacctt ggtatactca aacagaccaa
     2821 ttggttatat caggagaatc tataattaga aacctttact atggaataga tagatgcaat
     2881 gaatttggtg aacctatgag ggttggatat gtaccagatt cttttgggca atcagctcaa
     2941 atgccacaaa tatacagagg ttttggaata gatagttcat tattttggcg tggagtttct
     3001 gatgatatgg tagatacaac agagtttata tggaaaggtt ctgatggttc aaaagtatta
     3061 gcagttcaaa taccatttgg ttattactat ggaggtaata taccagaaga tgattcagat
     3121 ttaaaagaat atttaaaaga tataattgga aaacttaagg ttaaagcttc tactaataac
     3181 gtatattttc caaatggatt tgaccaagca ccaataagaa aaaaccttcc tgatatatta
     3241 aaaaaagcta atgaaataga taatgaaaat gaatacgaaa taagtaatat agaaaactat
     3301 atagattcag taaaaaatga aagaaatgaa tttacagagc ttaaaggtga atttttaaat
     3361 gctaaacata tgagaataca caaatccata ttctcctcaa ggtcagacct taaaataatg
     3421 aataacaaat tagaaaatta tatcacaaat gtacttgaac cattattaac attaagttat
     3481 tcacttggaa atgaataccc tcatctagtt ataaaagata tatggaaact tatgtttgaa
     3541 aatgcagctc atgacagcat aggttcttgt aatagtgaca cagcaaatga ggatgtatac
     3601 acaagataca aacaagccag agatttgagt ataaatttat tagatttgca tatgagactt
     3661 ataagtactc aaatcaaaaa taataatgaa gaaataacat taacagtatt caatggattc
     3721 ccagaagtca gaaatgaagt tgtagaattt gacacttata ttccaggtca agattttgtg
     3781 ataaagaata aaaatggaga aattcttaat tatataataa aagaaaaaga agatattaca
     3841 aattatatat taactcaaac aataagatta aacccaagta aaaaaatata tatacccaaa
     3901 actgtatata gagcaaaaat tgcactttca gtaaaagata taccaccaat gggatatact
     3961 caactagttt ttgaattgga tactaaaaat aaatgtgaaa tagaaacaag taattgcaaa
     4021 gaaatagaaa acaaatatta caaaataaca gccaataaaa atggttcatt agaaatatta
     4081 gataaagaat ctagtaagac ttataaaaac caagctgtaa ttgaagacaa tggagatgat
     4141 ggagattcat acaactattc tcctccaaga aaagatattt atgtttcatc tttagattct
     4201 gtatcttcag tagaagtgct aaaatcaaat attcaagaag aaatgatact taaatattca
     4261 ttgaccatac cagcttcact ggaagaaagg tctataggta aagtttctgt gaaaatgcca
     4321 gtaacaatga aaattacctt agaacctaaa gaacaaataa ttaagtttaa tgttaatata
     4381 aaaaataacc aagcactatc tcatagagta agagttttat ttaatacaga aatagcatct
     4441 aagttttcta tagcagatca acaatttgga ataatacaaa gaccaacagt attagaaaaa
     4501 gaccttgcat tatggaaaag agacaattac tcatggcaag aaaaacctat aactatcgaa
     4561 ccaatgcaaa gttttgttac tttagaggat ggacaaagag gaatagcatt aataactggt
     4621 tgtgtaagag aatatcaaat agttggtgat aatttagata caatagcatt aacactattc
     4681 aggtcatttg gatatatggg aagagaaaat ttactttata gaccaggaag agcttctgga
     4741 gaaaaaataa tagcaacacc agatgcacaa ttacttaaag agttaaactt tgactttggt
     4801 ctttacatat ataacaataa atttgatgaa gcaaatgtag ctaatacagc taagagattc
     4861 ttaacaccta tacaaatata tgaatatgct gattttttaa atggtagact tctatttgca
     4921 ttcaatgacg aaaaacaaat atatgaaaat gaatttagtt tgatgaatgt aaataattca
     4981 aatttcacag taagtgcaat aaagaaagaa gaaaaaggag atggaattgt agttagaatc
     5041 ttcaatggta tgaaaaatga ggatgctaag ggaagtctta acatcaataa aaatgtcaat
     5101 gatgcttata gtgcaatgct cgacgaaatc tatgataata atagcaagct taaagtaaat
     5161 actaaaactg tagaggtaaa ttctttatca cattgtaaag tacaaacaat agtaattaaa
     5221 taatataaaa tagcttatac aagatttcaa aataaattgc tagtactatg ttaatagtaa
     5281 acccagatat tatgctattt ataagctctg taacactaaa taacattttg taaaataaaa
     5341 aggtgaacct tgtatattag gattcacctt ttttaaataa atcaacattc tgttttaaaa
     5401 taacatttat aagctgtttt gaaataatac ttacaaatta ttttaaaaca atacctataa
     5461 atttttattt aaagaataca cataaatatt tccagactct tttattctat agactttata
     5521 attattaaag aagtctaatc taatttttgt taaaagtaat tgtaacttta aataaatcac
     5581 catccacact tatattcatc ttaccaccct gtaattctat aaaacttttt gctattgcta
     5641 aaccaagacc agaaccttct gtgttacgtg atttatctcc tcttacaaat ctctccatta
     5701 tatcatctac actaaaatca atctcagatg cagacatatt tttaagcata atatttactt
     5761 tattatctgt ttctaaaata tcaatataca ctcttgaatt tggcattgca tatttgctta
     5821 tatttattat aagattttca aatactctaa atgtacgctg actatctaat ggtaatatta
     5881 ttttttcatt agaaaagtta ttctttacta ttagagatga atctgcaagt ttgtcatcta
     5941 attctaaaag tgtctgcttc attagagata caatatccac atttactata ttcaaataaa
     6001 catctccact atttgccttg cttacttcaa acaaatcttc tattagatgt tgcaatcttt
     6061 gtgactttct gtctaatgta tcaatataca acttacgatt ttcatctgtt atactttcat
     6121 cttttaataa atctatataa gtgattatag atgttagagg tgtttttaag tcatgtgata
     6181 catttgatat aagctctgtt ttcatctttt gactcttaac ctcttcgtca acagcttttt
     6241 taaatccagt ttgaatattt cctaattcgt cttttatagg attaaaaaat cctaaatctt
     6301 tttccatatt tacatctaag tttccatctg aaattttctt tgtaacatct aaaagttgat
     6361 tatattcata tattgtcttt ttagagtaat ctgacagtat cttaaataat actactgaat
     6421 atattaatgc gagtattatt ccaaagaacc acatactaca cattataact ataatcaata
     6481 aatttattcc caaaattgta actatagttt tatttggctt ttcttttaga tttattgttc
     6541 taatttgatt aataactgtt tttcctgctt tttttaacca tacaaaaaac attcctgtca
     6601 cagtatgttt tgtaaaatac tctcttattc caacatttaa aatatgtttt aaaagtatta
     6661 taaatacaaa tattattgag aatattacaa accaataagt taaattcatt aaacttacca
     6721 atatgctagc cattttagca cttatgtcat tgtttataag gtactctaca taattcccac
     6781 tcaaggtatt acttattata acagtaggat ataaatctac aagcccctgt ataatagcaa
     6841 gagcaataaa gagtatctct aacggtattc ttaataatgt atcaaatcct agcaactctt
     6901 tagattttct atatgggaat gctaaagcta caataacaac tatagctaat ataattttta
     6961 aaatcattcc agaaatctta taataagaat tattttctac attgcttata tatgaaatta
     7021 taccatcatc aaaataagta tttccataac catactctac actatcacct ttacttatta
     7081 ctttaggtac agcatatact aatgttatat tttttattgg agataactta tatgaaattg
     7141 aatttcctcc taaacttgta tccattaaat cttctcttgc tttagataac aacatttgat
     7201 taataacttt ttgatttact ccataagaat ccacaatatt tacttttcca tctttatcaa
     7261 aattcattac aacgtagaat ctatagtttt cctttatatc tgtaatctgc ttttgtcttt
     7321 cctcatcatt catttctgta ttttttgact gattttcatc agaattttct tttgaagcat
     7381 cattctttgt tgtatcaagt actcctaatt ctttattatc attaaaaaat tcaaaattgc
     7441 tttctttatc aactaaatag tactttaaat tctttcctga cgctttaatg aaatgctccc
     7501 aattttcaaa gttttcatta aaatctgcat taatactatc atctgtataa taagtttctg
     7561 aaccatcata agtatctttt tctttactta cattttttat atagttcata gtttcttcat
     7621 tgataaatag ctttgatggt tgtatcatct taccttcttg ttcttccttc atattatgat
     7681 ataaaacata acttccttta tatatactat ctacaaagct ttcctgttca aatggattct
     7741 ttgctccacc tttagcacct gcttgtatag tagaatattg actaatcata cctaatgatg
     7801 ctgataacat aagtataaaa actataatag taactatata tttatcttta aattttttca
     7861 attttgtatc caacacccca caccaccttt aaatatttag gattttttgg gtcaatttct
     7921 atcttttccc ttatattcct cacatgtacc ataactgtat ctgtatttat agcattttca
     7981 ttccaaactc tttcatatat ttcatcagca gaaaaaactc ttcctgcatt tctcataagt
     8041 aaacttagta ttttaaattc tataggagtt gtttttatat attttccatc aatattaact
     8101 tcttttgtat tttcatttaa ttctaatccg cctattgcaa acacactatc atcactttct
     8161 accttttgct ctttatttcc aaccatgttt agatacttag tgtaccttct aagttgtgaa
     8221 ttaactctag ccaatagttc taatggctta aaaggttttg ttacataatc gtctgctcct
     8281 atattaagcc ccattatttt gtcaacttct tctgattttg cagatagcat tattactgga
     8341 aaattatagt tttctctaag cctcattgta agtgttatac catccatttt aggcatcatt
     8401 atatcaacta tagctaaatg tatttcttgg ttttctataa ccttaagacc ttctaatcca
     8461 ttgtatccct taaaaacatt atatccttga tttaatagat atatctctat agcatctgcg
     8521 atctcctttt catcttctac caccaaaata ttatatgaat tcattttaat ctcctttata
     8581 ttaatttata aattttcttt acatatatga ttatacaata tgatagaagt ttttccattt
     8641 atatatatat ttgtatcttt tgttctggtt atttgtattt aatgtccatc ttagaaacct
     8701 ccttctagtt taaacttaat attttttcat tttgttttat atacttaata ttaaaattaa
     8761 aaacttaaga t
//
LOCUS       DAEPOZ010000153         8766 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.153, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000153 DAEPOZ010000000
VERSION     DAEPOZ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8766
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.153"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(121..981)
                     /locus_tag="KR440_002628"
     CDS             complement(121..981)
                     /locus_tag="KR440_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HBH0699663.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDI
                     EEVEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
     gene            complement(1298..2626)
                     /locus_tag="KR440_002629"
     CDS             complement(1298..2626)
                     /locus_tag="KR440_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBH0699664.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            complement(2774..3103)
                     /locus_tag="KR440_002630"
     CDS             complement(2774..3103)
                     /locus_tag="KR440_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBH0699665.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            complement(3127..4416)
                     /locus_tag="KR440_002631"
     CDS             complement(3127..4416)
                     /locus_tag="KR440_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0699666.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            complement(4432..4758)
                     /locus_tag="KR440_002632"
     CDS             complement(4432..4758)
                     /locus_tag="KR440_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0699667.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            complement(4893..5522)
                     /locus_tag="KR440_002633"
     CDS             complement(4893..5522)
                     /locus_tag="KR440_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699668.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            complement(5737..6753)
                     /locus_tag="KR440_002634"
     CDS             complement(5737..6753)
                     /locus_tag="KR440_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0699669.1"
                     /translation="MVKAVGDIIGKCSAISFDMKLDKSYDEVIVDFKNLINNIDNKDV
                     LLFTDMGSLNSFDEIIKKEKKCEVRVIPMVTTLTVLEAVQKANMGLPLNDVYNSITNT
                     RKYYFGTNEIQNKENLSKTIIIASHVSEGVDNKTRKILEEKMSRYLDGIDIISVPYKT
                     EKDLSLNITKLKESSNIVAVINEQRINIRGIDYISKKDIDKDENINKLKNIIKISIGY
                     DDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLVFNIEKKYDKVIGLMMHLAFMVDGLI
                     GNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDKKYNIEINEKECYQILLILEYAEIIE
                     KDYQ"
     gene            complement(6734..8392)
                     /locus_tag="KR440_002635"
     CDS             complement(6734..8392)
                     /locus_tag="KR440_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0699670.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERDSKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVRAKQDEQNKIVNNINIIVAMHGETTASNGKSSW"
BASE COUNT         2694 a         1544 c          894 g         3634 t
ORIGIN      
        1 cctacttatt tattaaaaat acaaattata ttttattaat ttttactact tgtaaattat
       61 tatcttatac aatgaatttg tattatataa taagtatttt taggattctg ttacataata
      121 ttatttaaat attcttctat atttctcttc ttcttcttca gtaaacacat taaatacaac
      181 ttttaaattt ttttcaggat tttcttttag ccatagtcta actctactta cagcaagatt
      241 agctgcctct ttttttggat aaccaaatac acctgtagaa attccacaaa aaacaatatt
      301 ttttatttca tcaatttctt ttattgtatt taaacatgat ttataacaat gaagcagttg
      361 cttttcttgc tctttacttg gctgaccacc agatacaata ggtccaacag tatgtactac
      421 aaattttgca ggtaaacaat agcctctagt tatcttagca tctccagtat attctaaatg
      481 cccttgtttt tttattattt tgtcacaatc ttctctaagt ctaggacctg cgcatgaatg
      541 tatctcatta tccacgcata gatgtaatgg ttgtaaacac cctaataact tgttatttgc
      601 agcatttact attgcatctg ctcttaaatt cgttatatta cctctccata tggcaatccc
      661 ttcctctact tcttctatat catttacatc tactaactct ctttcttcac attcaatcaa
      721 caataaatta tgtaacatat taagtatctc tggagcaatt tcttttggtg gatatacatt
      781 caaaagtcct ctaagtatat ctctaggctt aatttcatca tttgataaat tgtatatttt
      841 agaaggaact tcttttgaaa aatatgcaat taaagtgttt atattttttc ttctttgttc
      901 gtcagttaga ggcttgatag ttttatcaaa atcctcaaat aaatttacat catcagcata
      961 atctctccac ttcatagcca tctctccatt cttgtatttt agacttaaaa ataattatgt
     1021 atttatgtac atatttattt atttctataa acacctacat aaatcctgtt aaaagtgtaa
     1081 attttttatt atgcaacttc tctggttatt tctattgatt gatagtaaat tattgtacct
     1141 aaatttaatc caactaacaa gtaactaaac ctactttcct aactgcaaaa tacactatgt
     1201 ttgccacttg tatctcattt gtaaataaat aaagtcccta caaatacatt aactaggcta
     1261 tgtactaaac tgtaggaact ttaaaaatat attattctta attaagtttt ggccaataat
     1321 ttttatttgc ttctattaaa tcatctagta attcttttgc aactgttgca ctaggaactg
     1381 tcttagatag cataagtgct tgccacaatt tattataaga cttctcaatc caagcatcaa
     1441 ctactaattt ttcaactgct aattgttgtt ctattaaacc tttttggaat gttggtattt
     1501 tccctattga taatggttca tacccgtctt ttccaactat acatggaatt tcaaccatag
     1561 catcatcttg tatattttct atagctccat tattttgaac tatcaataac atacgctcct
     1621 gtgtattaaa agctagagct gtagctaaat ccactataaa ctctgcatgt attcctatct
     1681 taagcccact gcccttagaa tgtccttgct tttctatcaa tgcacatgaa ccaaaaactt
     1741 ctttttctct tccatcaata acttcattgg cacgagtata atttacatca gaatgattta
     1801 caacataatc tggaaataga tagtacttta agtaactact tggtattgta gttaaatctg
     1861 tttttactat ttctttagta aataaattag tttcaaacca actatctttc ttgtcatttt
     1921 tcattccttc ttcaccattt ataaaaccat attcttttat atgttctact aattttggta
     1981 aaagttcatt tcctttttta tctttaattg aagtatacca accaaagtga tttaatccaa
     2041 agtatcttat atccatatgt ttacgagatt tgagtcctgc aatccttgct atattatgtt
     2101 ccatacctat tggcatatca caaatattta ttaccctaga attaggtctc aaccttctaa
     2161 cagcttccgc tattattgat gctggattag aataatttaa catccaacaa tttggagaat
     2221 acttttccat ataatctatg ttttcaagaa caccttctat agacctcatt ccataagcta
     2281 ttccacctgg tccacaagtt tcttgaccaa ctactccatg ttttagaggt atcttttcat
     2341 ctagttctct catttcaagt tttccaactc ttatatgagc taaacaaaaa tctacatctg
     2401 tataagcctc tttagggcat gttgttgcta aatactctat attagggtcc ttttccttta
     2461 ttagtatttc acaagcttta gctaccttat tttgtctttc ttcatcatta tcatataatt
     2521 tgattgctct taatggtaac ctatctaagt tatctagcaa cattaatatg atttctgggg
     2581 tatatgtact tcctcctcca gctattacta ctgaaaattt ttccataata atatctcctt
     2641 ttctatttaa aaatttactg tatttataaa taaatttata aatactagta ctttaaagta
     2701 aaagggagtt catttattaa atctattgat ttaataaact atagccaaac ttcaagcact
     2761 atttacaaga aatttaattc ttattaattc ttgggtccat catctcaaat atatctacaa
     2821 gttcagatac taaagaatga gcaaataatg catttgaata atggtcttca gagtgcacaa
     2881 ctaaaaaatc agtcccacta tcttcactat tcatagcatt tgatagtaat tctaaatgat
     2941 gctttgatgc tgtttgtatt ttctctgagg ctgaatttaa aaggtgtttt gctttattgt
     3001 agtcaccttt ttttatttcc ttaagagaca tcattacatc acttttagca tctcctgctt
     3061 ctgatattat actaaatact ttttcttcca tttcaggtac catatccttc tccttctaat
     3121 gacttgttaa aaatcaaagc tatcatcatc taatgttgta gctaattctg catttttatt
     3181 taattcttcc ttctccatta tcttaaagaa tggataatat atcaaggtca taagtattat
     3241 acttgcaaat actaatacga ttgccttcca atctaatgtc atcaaaaatg ctgcaacccc
     3301 tggaggtaaa aagcctggtg gttctgcaat aggtaatcct actaatccta cttttaatgc
     3361 cataaaatta agtgctccaa ccaatacagg tccaaataca aatggtacaa acatatatgg
     3421 gttcataact attggtgttc caaatattac tggctcattt attccaaata gtgatggtaa
     3481 aatcacaact tttcccatac tattaaaact cttacactta cttcttgaca taaggtacac
     3541 taatcccaaa gttatccctg aaccagttac ttgcaagaaa ccatagtaaa atcctattgt
     3601 aaacacatat tttgggtctc ctcctgctgc tatcagtgtt gcattttctg ccatatatgt
     3661 agctgctatt ggcatccata tacttgaagt tattgctgct ccatgaaggc caaagaacca
     3721 taataattga gttataaata gtattatagt tactgcaaaa acattatcta cactgcttat
     3781 agctggtgct aagaaattca ttataatagc tggtaatggt tgacctgaga catttttagt
     3841 tattgctgct attgctgtaa ataacattat acatactatc attggagtta aatttctaaa
     3901 acttttattt actacactag gtacttcttt tggaaatcta attacgagat ttttatcatt
     3961 cataaactta gtaatatcaa ctactaaaag tgctactaat atacttacaa ataaaccttg
     4021 tgcatctaaa taacttgtat ctaatacacc atctactact tgtccacata caacaagttg
     4081 tgcaacaact gctgtcatac ctatttctac atgattaaga ttatattttt tagctagtgc
     4141 ctgagctata aaaaatgctg aatatataga taataaactc atagtaaatt tattagggat
     4201 aagtaatgta tccatatttt cagataataa tgtttttgca cttccttcag gtaacatatt
     4261 gtttaatgcc ataaataaca tacttaaaga accaacaatt attattgggg taatgcttat
     4321 cataccttct tttatagctt gaagatgcct ttgtttttca atcttttgtg ctattggtag
     4381 aagaaatctt cttaaattat tttgaagtgc ttccattttt attcccccca attatctatt
     4441 tttatataat tcgattgccg acttcataac agaaggagca tccatacgac cataatgctt
     4501 agtatctatt aaaccaactg ctattttgta attcttacaa gcttcaacta catcatcttc
     4561 cttgaaccta atttgtggtg ctacaagtac tacatctgta tcattagctt caatgtatga
     4621 ttttaaattt tcgaatgatg ttgcctctat atttatagtt ttagttttta acttttcatg
     4681 ctcactacaa aaacttttca tcttttccac aagtacacct gttgaagctc catttgcaca
     4741 tactaataaa atattcataa ttaccctcct acaatatcta acctctttta ttttctaatt
     4801 ttttatcagc caattcttac tctaattcct atcagttttt actttaattc ttattatttt
     4861 attttaattc ttattaatat ttactctaat tcttagtact gccaatgatt aaacttgaaa
     4921 gccgtatcta taacttcttc ttgcttatat ttttctaata tttttaagta tctttcttta
     4981 taatcattat ctacttcttt ttctggtatt actatactag cttcatttaa aaagtggaat
     5041 gaatcccttc ccatttctaa tcctctatat gtatgcttgt caagtgccac gtccataact
     5101 tctggaactt tacccattgc aaatcctttt ataattatat tttttaataa atcacttgat
     5161 ctatctttct cacatgcaca aagatatctt attgcatgta tgaaaaataa aggtctgtct
     5221 ccatctgcat atgaatattc ctttcttact tgatttaaat tatttatgat tattacagct
     5281 gatgggtccc ccattcctat atcttcaact gatattgtaa gtatccttct ccatagctta
     5341 tcttctaatt ctggtgatga tatatatagt tcataagcaa attcacaagc ttcttccacc
     5401 atatttcttc ttatagcttt ttgaaatgac gatattattt cgtctgcact aaatccgttt
     5461 cttgtagtca ttcttgccca tgggtcattt gcctttttat ctcttaatat tttatcttgc
     5521 atattattta ctccttttta tttaaatttt aattatttat attactcatt taataatata
     5581 agcaagtatt atgccagttt ttttagacac tttaaaacac tgttatttca atcattttta
     5641 tcatcaaaac ttagatactt tgtctaagta tatttatatg tgtctaatat taatattatt
     5701 ttagactaaa aaaaattacc aaataagttt ttatactcat tggtaatctt tttcgattat
     5761 ttctgcatat tctaatatta ataatatttg atagcactct ttttcattaa tttctatatt
     5821 atatttttta tctaattgag atactatatc ttttattttg cttaggctct tgtgataatc
     5881 aagtgttttt tctttatcaa gcttttcaat ttctctagta tttccaatca acccatctac
     5941 catgaaagct aaatgcatca taagtccaat tactttatcg tatttctttt ctatattaaa
     6001 tactaaaaac aattcatcag acacatattt tatatcttta aatatcctat tataattaga
     6061 gctttttaag gatgtcttta aaccttcaac tacatcatca taacctatac ttatctttat
     6121 aatatttttt aatttattga tattttcatc cttatctata tcttttttgc taatatagtc
     6181 aattcctcta atattaattc tttgttcatt gattactgca acaatattac tactttcttt
     6241 aagttttgta atatttaaac ttaaatcttt ttctgtttta tatgggacag atataatatc
     6301 tattccatct aagtacctac tcatcttttc ttctaatatt tttcttgttt tattatctac
     6361 accttctgaa acatgtgatg ctataataat tgttttagac agattttctt tattttgaat
     6421 ttcattagtt ccaaaataat actttctcgt gttagtgata gagttgtata cgtcatttaa
     6481 aggtaatccc atattggcct tctgaactgc ttctaacaca gttaatgtcg tcaccatagg
     6541 aattactctt acttcacact ttttttcttt ctttataatt tcatcaaagg agtttaatga
     6601 acccatatct gtaaatagta atacatcttt attgtctata ttattaatta aatttttaaa
     6661 atctacaata acttcatcat agcttttatc taacttcata tcgaaagaaa tagcactgca
     6721 ctttccaata atatcaccaa ctgcttttac cattacttgc tgtagtttct ccatgcatag
     6781 caactataat attaatgtta ttcactattt tattttgttc atcttgttta gctcgtacta
     6841 tgaataatgt aatatatgct acagtttcta tagataaaaa taaatcaaat tcattttcaa
     6901 ttattccaac tatttttagt gctgttttaa attcacttct gttatttctt ctaatttgat
     6961 tcaaatctat gttgtttgta gagctacttt tatccaatac gctttttata taaattgcca
     7021 ttgacttaaa tattgattca cttatatcta tttcttcatc ttctttaata atttgtctta
     7081 ttgattcaca catgctttta aacttaacat tattaaatag agaagcatct gctatattca
     7141 tttctacaga tttattaaga ttagtattta ctgcattaat aatatttata tcattgtagt
     7201 taataacttc ttcttctcct aatgacgtaa atacaaaata ttctattcca tgtgttataa
     7261 cttcatctgc tttaatagac ttctctctat aatcatcact cacttcattt atagatttat
     7321 caaaatattt tttctctata taaactggtt ctttattatc tcttttatat cttaggtatc
     7381 catttgcaac agctaatttt atatcatttt gaagttgacc tacattattt ttacaatcat
     7441 aaagtaatag tgatttaatt atttctttat gtacttttat tgaaatagaa atactttttg
     7501 attcttcctt gaaaaaattt ttgactaatt ccattctttc aatcattgtc ctatctctca
     7561 gtgatggtat ttctattttc attggtattc ttctattgaa tgtatctaat agtgttgatg
     7621 tcggattttc tgtagtagca catattatta ataccttaga atctctttct ttcgttgcat
     7681 ctcctagtct tttaaatgtt cccttatcta taaaagtaaa caacatttct tgaccttctg
     7741 ctggtagtct atgaatttca tctaaaaata gtatccctcc atttgcaatt tccaaaagac
     7801 cttttctgga agattctgca cctgtataag ctccttgttc tactccaaaa atatgtccca
     7861 taagtaattg tgcattatta gcataatcag cacaattaaa tgttacaaaa ggagctttat
     7921 tttttctaat tccactatct atagaataat cgtacatata tctagcaatc atactctttc
     7981 ctactcctgt ttctccatag ataatagtat gaagtccatt tgtaggatag agcattgcga
     8041 ccttgcattt ttctattata ggtcttaaac tatgttcttt acctattagt gtttcaaaac
     8101 tatctttttt tattttgttt tctacttttg ttcctttttt tatgccaagc aactcacttt
     8161 gtgtatcaag caattcattc ttgttaaggt taagatttgc agggatataa tacacaggtc
     8221 ttccttttat cttcgtaacc ataccctcat tgaataattg gtttaaatat gtacttatat
     8281 tgtttcttgc aattcctgtc ttttctgata attggttagc tgtaatccct ttttcatttt
     8341 ccctatatga tgattctaaa actttcatta ctttatcttt tttatatatc attacaagct
     8401 cccctacatt ataatatttt attaactaaa acccctatac acactttatt ttttaataaa
     8461 ctcttattct ttaactaagg ttataattta tcaaactttc cttactttaa attatagaaa
     8521 attattgtag aaaaaataag attattgctc attttttcta cattactatt tttaatttta
     8581 tagcactttc ttaacaacat actttacttg tttttaattt tattatattt gaatttatta
     8641 tatttgaaaa gtagaaataa aaaaccctat tttggagtag ttttaaaatt tctccaaaaa
     8701 tagggtttta gtttgtattt caaataaata cctgttcaag tatttttata ctaaacaact
     8761 tttaaa
//
LOCUS       DAEPOZ010000154         8737 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.154, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000154 DAEPOZ010000000
VERSION     DAEPOZ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8737
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.154"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            29..985
                     /locus_tag="KR440_002636"
     CDS             29..985
                     /locus_tag="KR440_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HBH0699671.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFIGIIIFKIKKLL"
     gene            1162..1317
                     /locus_tag="KR440_002637"
     CDS             1162..1317
                     /locus_tag="KR440_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699672.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            1447..2460
                     /gene="aroF"
                     /locus_tag="KR440_002638"
     CDS             1447..2460
                     /gene="aroF"
                     /locus_tag="KR440_002638"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBH0699673.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKIVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            2482..3546
                     /locus_tag="KR440_002639"
     CDS             2482..3546
                     /locus_tag="KR440_002639"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBH0699674.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            3546..4859
                     /gene="aroA"
                     /locus_tag="KR440_002640"
     CDS             3546..4859
                     /gene="aroA"
                     /locus_tag="KR440_002640"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBH0699675.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLAFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNSTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            4837..5916
                     /gene="aroC"
                     /locus_tag="KR440_002641"
     CDS             4837..5916
                     /gene="aroC"
                     /locus_tag="KR440_002641"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBH0699676.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDKIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQRDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            5919..7115
                     /gene="pheA"
                     /locus_tag="KR440_002642"
     CDS             5919..7115
                     /gene="pheA"
                     /locus_tag="KR440_002642"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBH0699677.1"
                     /translation="MEDLTRYRNEIDNIDKEIIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAIDNLDNKAYSQETVQFFNSIMEISRGLQKRLIDNDGEQKIEFSLDS
                     SNKKFDLSNVNKYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKTISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSEFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYELEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAENNINMTKIESRPMKNASWRYFFYIDFECSIYNSKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            7162..7971
                     /gene="aroE"
                     /locus_tag="KR440_002643"
     CDS             7162..7971
                     /gene="aroE"
                     /locus_tag="KR440_002643"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBH0699678.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRIFEILNIESAYKNFEIPKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            7983..8516
                     /locus_tag="KR440_002644"
     CDS             7983..8516
                     /locus_tag="KR440_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBH0699679.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            8537..>8737
                     /locus_tag="KR440_002645"
     CDS             8537..>8737
                     /locus_tag="KR440_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HBH0699680.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSI"
BASE COUNT         3589 a          866 c         1543 g         2739 t
ORIGIN      
        1 atgtaaattg aaatttagga gtagatgtat gtatatactg aatttaaata caagagagac
       61 tattgaagat tttagagata aattttatgt agcagaaaat tcttatctta tattaagtgc
      121 tccaaaaaat ttaaaattgc ttaaggaaac tttggatata gatgaaataa cttttaatga
      181 ttgtcttaaa tttgatgaaa taaccaaact tgatttattt gataattacg attttttaag
      241 cctaaataca tttgaactaa gagatggaga agctgttata gaagaagtaa atatgtattt
      301 atcagataat tttatattgg ttgtagtgaa tgaagaacac tttttatttg aatttgtaaa
      361 aaatattatt ttgaagaact ctcaacttga aaaaaatcca gttattaatc tatttaagat
      421 aaactacttg attcttagag aagttataaa aaatggtttt gagtctttgg aaaaagttga
      481 agagttaatt cttcaaatag aagatgaaat gatggacaac attaataaga atcatgtatc
      541 tagaataagt gatgtaagag gattaacaag aattatagtt aagaatacta gacctctttt
      601 gtatattggg gatagaatag ttaaggaaaa tataagatat ttaaaatatt caaatgtaaa
      661 aaaatataat ttagaaaatt ttcaaggtat agattttgga attgacaaat tatacaattt
      721 cgcattgtca acacgagagt tagcagataa attattagat atttattctt ccagagtagg
      781 tgagaaaaca aataacttaa ttacaaaatt aaccttgtta acagccatat cagcaccact
      841 tacaattata actggtatat atggaatgaa ctttagatat atgccagaat taaactggat
      901 ttatggatat ccagcaactt tattttttat gctatgtata atttttattg gaattataat
      961 atttaaaata aaaaaattat tgtaaattac agatgatgtt taattgattt tataaaaata
     1021 catcaattga gaagctgatt taagtaaata aaaaaatatt ttaaattgaa aatatttata
     1081 gaaaatattg acaattatat tttctttgtt ataattatta aacaagaaga ataataaatt
     1141 ataaaaatgg aggataatac tatgaaagga atattaaaag aactaacagc aaaactttat
     1201 agcttttttt actttatgtt ctggggcttt tattttagcg ctggctattt cttttacaaa
     1261 aatatatgta cttcctttct ggtgaggcct tattttagtg attttgagca taagtaaatt
     1321 ttataaaaat gattatttta tatatttata taagatttag tgcttgatag attattttag
     1381 agaactcatt agtgagttct cttttttatt ttaaatataa aattataata ttgggaggta
     1441 gtaaaaatga tagtagtatt aaaaatggga gcagataaaa atgaagttaa aaaattaatt
     1501 gaggctattg ggagagaagg agtagaagtt aatcccatag atggaacaga attaactgtt
     1561 ttaggtttag ttggagatac tagtaaaata gatgccaaaa gaatagaagc aaataagata
     1621 gttgaaaaag ttatgcatgt agtagagcct tttaaaaagg ccaatagaaa atttcatcca
     1681 gaaccttcaa tcataaatgt taatggaatg gaaattggtt caaagaaaat tgctatgata
     1741 gctgggcctt gttctgttga aacagaagac caaatagtat ctatagctaa agatgtgaaa
     1801 aaatcaggtg caggattttt gagaggtgga gcattcaaac caagaacatc accatacgct
     1861 ttccaaggat tgaaatatga tggattggat ttattaaaaa aagcaaaaga aaaaacaggg
     1921 cttcctatag ttacagaaat aatgtcaaca caagatatag atatatttga agaaaatgta
     1981 gatgtaattc aagttggagc tagaaacatg cagaactttg atttattaaa agaattaggt
     2041 aaaacaaata aaacaatact tttaaaaaga ggattatctg ctacaattga agagtggcta
     2101 atgtcagcag agtatataat ggctggagga aatgaaaatg tagttctttg tgaaagaggt
     2161 ataagaactt ttgaaacata tactagaaat actttggatt taagtgcaat tttagctgtg
     2221 aagaaactta gtcatttacc agttatagtt gacccaagtc atgcagcagg taaatcatgg
     2281 atggttgact ctttatctaa ggcagcaatt gcagttggag cagatggtct aataatagaa
     2341 gttcataatg acccagcaca tgcactttgt gatggaaaac aatcgattaa gccaaatgaa
     2401 tatgatgaat tgataagtga attaaagact atcgcatcag cagttggtag agaaatataa
     2461 tctataaaag gatggttaaa cgtggaagaa aaactattag taaatttaaa agaaaatagc
     2521 tacaatatat ttattaaaaa aaatatacta aagaatatag gagaagaaat aaaaaaaata
     2581 tattttggaa ataagatttt tattgtaact gatgaaaata tagcaaaatt atacttggat
     2641 aaagtaaaaa ataatttaag tgaatctgga tttgaaatta aatctttaat attaaaatct
     2701 ggtgaagaaa caaaatcatt taatactctt cctaaaatat acgatagttt acttgacttt
     2761 aagttaacta gaaaagattt gattattact ttaggaggag gggtaatagg tgatttaggt
     2821 ggatttgcag cagcaacttt tttaagaggt ataagtttta ttcaaattcc aacatcttta
     2881 ttagcacaag ttgatagttc tgtaggtgga aaagttggag ttgaccttga gagaggcaaa
     2941 aatttagtgg gaagctttta ccaaccaaaa gctgtgttta tcgacccaaa tgtattatta
     3001 tcacttccag aaaaattcta tagagatgga atggcagaag ttataaaata tggatgtata
     3061 aaagacaaaa cttttttcaa taaactttta agtttaaaaa atagagaaga agtaatgcaa
     3121 catatagaat acattataca taaatgttgc tttattaaaa aaactgtagt tgaagctgat
     3181 gaaaaagact ttggagatag aatgcttcta aactttggcc atacattagg tcatgctatt
     3241 gagaaatact acaattttac gggttatacc catggagaag ctgttgccat aggaatgtat
     3301 aagataactt tagaaagtga aaagatagga attacaaaaa aaggtacatc agaacttata
     3361 aaatcaattt taatgaatta taatttgcct tatgagattg aattaagaga taataaatct
     3421 atagtagatg ctatatctct tgataaaaaa aatctaggtg atacattaaa aataatactt
     3481 ttaaaagata ttggagagag tattatatat aatactactt ctaaattttt tcaggaggta
     3541 agataatggg aagtctaaaa atatatccaa gtaaattatc tggagatgta aaaataccac
     3601 catcaaaaag tatggcacat agggcagtta tttgttcatc tctaagcaat ggaaaaagta
     3661 gaatttctaa tatagatttt tctgatgata taatagctac tataagagca atgacatcat
     3721 taggagctat tatagaaaag aaagaagata ttttagaaat ttcgggaatt ttttctaaag
     3781 aaggtatttt aaatagagag aatcagttga atcaacctaa attgacaata gattgtaatg
     3841 aatcaggctc tacattaaga tttttagtac ctatatcttt agcatttgat ggtgtaaaaa
     3901 ggtttatagg tagaggtaat cttggaaaaa gaccacttga tacttattat gagatatttg
     3961 acagacagaa tataaaatat agttacaaag aaaatcagtt agatttgatt atatcaggta
     4021 agcttaaacc agatgaattt agagtaaaag gaaatataag ttctcagttt atcacaggtc
     4081 tattgtttat attgcctaca ctagagagtg attcaaagat aataattact actgagctag
     4141 aatctaaagg atatttagat ttaactcttt caacgataaa agattttggg gtagaaatta
     4201 taaacaataa ctataaagaa tttattataa aaggaaatca aacgtataaa gcgagagatt
     4261 ataaagtaga aggagattat tctcaaggag cattttattt atcagcagat gctataggag
     4321 aagatattag catattagac cttaaagaag attctcttca aggggatagt gaagtagttg
     4381 aaattttatc taggatggga atggaaatat taagagaagg caacaaaata aaaggaataa
     4441 caaatggttt aaatagtact ttgatagatg catctcaatg cccagatata atacctgtat
     4501 tatctgtagt agcatcctta agtataggga ggactacaat aattaatgct ggtaggctta
     4561 gaattaaaga atgtgataga cttcatgcaa taaatgttga gttaagtaaa cttggagcaa
     4621 atatagagga gaaagaggat agtttgataa tcgaaggagt aagcaagtta aatggtggag
     4681 tagaggtgtg gagtcacaaa gaccatagaa ttgctatgac acttgcaatt gcttcttgta
     4741 gatgtgataa accaataata ttaaaagatt ttgagtgtgt atcaaaatct tatccacatt
     4801 tttttaaaga ttttaagatg ttaggaggtc gaattgatga gtggaatatg gggaaataat
     4861 ttaaaagtat caatttttgg agaatctcat ggaaatgcta taggtataaa tatagatggt
     4921 cttccttcgg ggattgaact tgaccttgat aagattgata aagaaatgaa gagaagagca
     4981 cctggaaaaa attctatatc tacttcaaga aatgaaagtg atataccaga aattctaagt
     5041 ggatatttta atggaagaac tacaggaacg ccattatgtg caatcataag aaatagtgat
     5101 actcgttcaa aagattatgg agagcttaaa aatttaatga gaccaggaca tgcagatttc
     5161 acaggtaatg tgagatattc tggatttaat gactatagag ggggaggtca tttttcaggt
     5221 aggataacag ctccattagt attttgtggg gctatatgta aacaaatttt atcacaaaaa
     5281 ggaatagaga taggagcaca cataaaaaaa attaaaaata tagaagatat gagttttgat
     5341 tatgtaaata tttcaaaaca acagttatca aatttgcaaa ctttggaact acctttactt
     5401 gatttatcaa aggaagaagc catgaaaaat acgattatag atgcaaaaaa tcaaagagat
     5461 tcagttggtg gtataattga gtgtgcagta gtaggtatta atgtagggct tggaaatcca
     5521 ttttttgact ctgttgaatc tactttatca catttattgt tttctgttcc tgccgtaaaa
     5581 ggtgtagagt ttggtttagg ttttgaactt gcagatatgt atggctctca aagtaatgat
     5641 gaaatgtatt atgaaggaaa tcaagttaag agcaaaacaa ataacaatgg agggattata
     5701 ggagggatta ctactggaat gccaataatt ttcaaagtgg caattaagcc aactccttca
     5761 attagcagac aacaaaacac agttaatata aaagataaaa aggacgatat tttatatatt
     5821 aaaggcagac atgacccttg tatagttcaa agggctatac ctgtaataga agcagtaaca
     5881 gccattggaa tttttgattt gatgaaagga cgataataat ggaggattta acgagatata
     5941 gaaatgagat tgacaatata gataaagaga ttattcaatt atttgaaaag agaatgaata
     6001 cagttcttga aattgccaga cataaaatga aaaataacac tactatactt caaaaagata
     6061 gagaagaaaa agtactaagt agagccatag ataatttaga taataaagca tactcacaag
     6121 aaacggtaca gttttttaat tctataatgg aaattagtag aggattacaa aaaagattga
     6181 tagataatga tggtgagcag aaaatcgagt ttagtttaga ttcatcaaat aagaaatttg
     6241 atttgtcaaa tgtaaataaa tataagtctt taaaagatga attaaataaa aaaaatatcc
     6301 tagtaggatt tccaggaaaa tcaggttcat ttacagaaga agcactaaat aaattcttca
     6361 acaaaacaat atcaaaaaaa caatttaaag aatttgaaga tgttttcata gcacttaaaa
     6421 ataaagaaat aaactatgga ataatcccca tagaaaattc atctacagga gctatctctg
     6481 aaacttatga ccttttgaga aagtacggtt tttatatagt tggagaagaa tgtataaaaa
     6541 tagaccaaaa tttaataggg ataaaagaca caaaacttga agatataaaa gaaatatatt
     6601 ctcatccaca aggtatagga caatgtagtg aatttttaaa acaaaatagt acatggaaat
     6661 tgataccatt taataacaca gcaacaagtg cagaacttgt aagaaaactt caagataaaa
     6721 caaaagcagc aattgcaagc aaaaaagctg caagtattta tgaattagaa ataataagtc
     6781 cttgtataaa tgatattgca aacaactata caagatttgt agtaatcagt aatcaaatac
     6841 acattgaaga agaatcaaac aaaatgagtg tagtattttc tgttgaacat gaagcaggaa
     6901 aactttataa agtacttgga tactttgctg aaaataatat aaatatgaca aaaatagagt
     6961 caagacctat gaaaaatgcc tcatggagat atttctttta tatagatttt gagtgttcta
     7021 tatacaattc taaagtgtac gacttattag aattgataga acataataca gcatatttta
     7081 aatttatggg agtatataaa aataaaactg aatagataat ataaattttt atttaattta
     7141 taattattag gaggtgtaag tatgaatttt ttcggtctag taggtgaaaa actctctcac
     7201 agtgtttcac ctcaaatcca caagagaata ttcgagatat tgaatattga aagtgcttat
     7261 aaaaactttg aaattccaaa agaagatata tctaagctag acggtgctat aaaattatta
     7321 ggaatacaag gagtaaatgt tactgtacca tataaggagc gtattatgaa atatcttgat
     7381 tttatatcac cagaggcaaa acgtatagga gcagttaata caattttact cagagaaaat
     7441 atgttatatg gttacaatac agattacttt ggtcttgata gtatgtttaa aatggctaat
     7501 attgatgttc aaggaaaagt agcagttata ttaggaactg gaggtgcttc taaagctgct
     7561 cttacatatt ttattgattc tggtatagaa aagctttatg tttcaactag aaaaaaggat
     7621 gataaaaaat tattaaatag taaagctata ttaatagatt atgaagaact taaacatata
     7681 aaaggagata taatattaaa tgctacacca gtaggtatgt accctaatgt aggaatttca
     7741 ccagtgtcaa aatctattat acaaaatttt gatatattaa tagatttaat ttacaatcct
     7801 ggagaaacag agtttttaag aattggtaat agtatgggta aaaaaacttg tgatggatta
     7861 tatatgctgg tagggcaagc aataaaatct caggaaattt ggcaagatac taaaatagat
     7921 aatagtattt tagatgttat atataatgaa ctaaaattag aatttttgta agggagattg
     7981 tcatgataaa taaaacaaaa gaaaaattga tattaatagg tatgccaggt agtggaaaaa
     8041 ctacaattgg taaacttttg gccaaagaat ataattgtag tttctgtgat atggatgatt
     8101 atataattca aatatctcaa aaaagtatag cagagttgtt ttcagaagga gaagatatct
     8161 ttaggaacta tgagactcaa gcatgtagag aactttctat aagtgataaa acagtgatat
     8221 ctacaggtgg aggtgtaata aaaaaggatg ttaatatgga aatactaaaa gaaacaggta
     8281 taataatttt tattgataga cctatacaaa agattttgga agatataaat attaattcaa
     8341 gaccattatt aaaaaacgga aaagatagat tgtataatct ttataatgag agaataaatc
     8401 tttataaaaa attttcagat atagagattt taaatgataa atcattaaat aatgctgtat
     8461 ataatattac aaatgcagtc agtgaaaatt ttaaatttga ctttaaagaa aaataatcta
     8521 gggggaaata aaaataatga atattgttat tgtaggacta ggagttatag gaggctcttt
     8581 tgctaaagca ttaaaaaaag ctggatatga aaatgtattt ggagtagatg tagatttaga
     8641 aactttaaaa aaagcagaaa aagcaaaaat cataaaaaaa ggatgtacta caggtaaaga
     8701 gttatttaaa aaagctgatt taattatact atctata
//
LOCUS       DAEPOZ010000155         8659 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.155, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000155 DAEPOZ010000000
VERSION     DAEPOZ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8659
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.155"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..363
                     /locus_tag="KR440_002646"
     CDS             <1..363
                     /locus_tag="KR440_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0699681.1"
                     /translation="LMISNGLIALSGSLMAQFLGFSDVNMGIGTLVLGIASIIIGITL
                     FKKFTFIKDTTAIIVGSFIYQFTIYFAMSLGMLSTDLKLITAIVIIAFLATGNLNISL
                     KKTNAKLVPKINQKKEVL"
     gene            364..1119
                     /locus_tag="KR440_002647"
     CDS             364..1119
                     /locus_tag="KR440_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0699682.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQSLSLIM
                     SCLTSPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALHYGDRL
                     IMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            1250..2272
                     /locus_tag="KR440_002648"
     CDS             1250..2272
                     /locus_tag="KR440_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0699683.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRIGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(2436..2993)
                     /locus_tag="KR440_002649"
     CDS             complement(2436..2993)
                     /locus_tag="KR440_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBH0699684.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPVKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(2999..4075)
                     /locus_tag="KR440_002650"
     CDS             complement(2999..4075)
                     /locus_tag="KR440_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBH0699685.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(4076..4759)
                     /gene="modB"
                     /locus_tag="KR440_002651"
     CDS             complement(4076..4759)
                     /gene="modB"
                     /locus_tag="KR440_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBH0699686.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(4908..5720)
                     /gene="modA"
                     /locus_tag="KR440_002652"
     CDS             complement(4908..5720)
                     /gene="modA"
                     /locus_tag="KR440_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH0699687.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKTDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEVESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            5913..6842
                     /locus_tag="KR440_002653"
     CDS             5913..6842
                     /locus_tag="KR440_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0699688.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            complement(7003..7452)
                     /locus_tag="KR440_002654"
     CDS             complement(7003..7452)
                     /locus_tag="KR440_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699689.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPHIYKKARVLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(7655..8635)
                     /locus_tag="KR440_002655"
     CDS             complement(7655..8635)
                     /locus_tag="KR440_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0699690.1"
                     /translation="MKNSFKNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDKPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMI
                     LVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKF
                     VKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPDILINYVVICGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
BASE COUNT         3003 a         1413 c         1058 g         3185 t
ORIGIN      
        1 cttatgattt caaacggact tatagcttta tctggtagtc ttatggctca gttcttggga
       61 ttctcagatg taaatatggg tataggaact ttagttcttg gaattgcctc tatcataatt
      121 ggtattacac tatttaagaa atttactttt atcaaagata ctactgcaat aattgttggc
      181 tcttttatat accaattcac tatatacttt gcaatgagtt taggaatgtt atcaactgac
      241 ttaaaactaa taactgccat agttataata gctttcctag ctactggtaa cttaaatatt
      301 tcactaaaaa aaacaaatgc aaagctagta ccaaaaataa atcagaaaaa agaggtgttg
      361 tagatgttac aaattaaaaa tctttcaaag agtttcccta atccatatgg tgaacctaat
      421 actatctttg aaaatttatc aatcgatata gaagatggtg aatttgtaag tatcataggt
      481 agtaatggaa ctggtaaatc tactttatta aatataatat ccgggcttat aaaagaatct
      541 tctggtcaag tattattaaa taaacataac ttgtctaatt tagctgaaca cagaagaact
      601 caaatagtaa gtagagtttt tcaagaccca agtcttggaa cttgcccttc tatgactgtt
      661 agagaaaact tgtctttagc tcttaacaaa ggaaaactat taaatctaag aaaatgtctt
      721 cgttacaaaa gagatttctt agaaaattta ttggaaggtg tatctcttga tttaaaaaaa
      781 taccttgatg ttcaagtaaa atttttatca ggaggtcaaa gacaatcatt atcactaatt
      841 atgtcgtgct taacaagtcc atcagtttta ttacttgatg aacatacagc agcacttgac
      901 ccaaaaacat caaatgaagt aattgaattg actaataaga ttgtaaggga aaaaaatata
      961 actacactta tggttactca caacttaaaa cacgctcttc attatggtga tagattgata
     1021 atgcttcata agggtgaagt tgtattagat gtaaaaggta aagaaaaaga acaattaaca
     1081 gttgaagaaa tcttagaaaa atttgaatat gcagtttaaa ataatatatc tattgtatta
     1141 aactcggctt taaaataaag ttaaaattac aataattatg gttactttgt aataattcat
     1201 atgaatatat gaacatatat aataataaat ttttgagggg gatataaaaa tgattaataa
     1261 aaaaagattg gcaagtttga ttttagctgg tgctcttagt atatctatgc ttacagggtg
     1321 ttcacaagga ggagactctg gtaattcaaa acaagaaagt aattctaaag gtaaagaagt
     1381 taaaaaaatt ggtataactc aactggttga acatccagca ttagatgcca ctaggatagg
     1441 ttttgtaaaa gcattagaaa agaacggatt taaagatgga gaaaatattg atatagactt
     1501 ccaaaatgct caaaatgaca tgcctactac acaaagtatt gcgagtaaat ttgcatctga
     1561 taagaaggac ttaatatttg ctatatctac tccatcagct caggcagcat ttaatgctac
     1621 taaagatata cctatactta ttactgctgt gagtgaccct gttgctgctg gattagttaa
     1681 aactcttgaa aagccaggca caaatgtatc tggaacatca gattttgttt ctgtagacaa
     1741 aggtttagaa cttcttaaaa tatttgctcc taaggcaaaa actataggag ttatgtacaa
     1801 tactagtgaa gtaaattcca aagttcaagt tgatgcttta aaagaatatg catctaaaaa
     1861 tggatttaaa gttgttgaaa aaggtataac tacttcaaat gaagttaacc aaggtatttc
     1921 tagtttagtt ggcaaaatag acgtattata tgttcctaca gataacttag tggcttcttc
     1981 tatgcctata gtctctaaaa tagctactga gaataaaata cctgttatag cagctgaatc
     2041 tgggccagtt gaaaaaggtg ctcttgcttg tcaaggtatc aactacgaaa aactaggtta
     2101 caaaacaggt gaaatggcag ttaaaatttt aaatggcgaa tcagtatctg atatgcctgt
     2161 ggctacatca gatgatacag atatcattgt taatgaagat atattaaaag ctttaggtat
     2221 ggaaaaacct tctaatgaaa atatttctta tgtaaagact aaacaagaat agaaagatat
     2281 tttctatata cgattaatat gcctgtttct ataaattaat ttatagaaac aggcatatat
     2341 ttttatttta atgtattatc tatttaaaga ttatatgtaa atgctcttaa tatgattgta
     2401 taaattttac ttgtataaac tttatacaat catatttatt cttctatttt ctttattact
     2461 ctacatggat ttccaacagc tacagtattt ggtggtatat cttttgtgac tacacttcct
     2521 gcacctatta ctacattatc acctatagta attcctggtg ttatgattac tcccccacct
     2581 atccatacat tatcacctat ttttacagga gaaccatact ctatgcctga atttctaagt
     2641 tgtgcatcaa ttggatgata ggctgtatag atttgaacgt ttggagctaa cattacattg
     2701 tcacctattt caattttaca tacatctaaa aaaatacaat cataatttgc aaagaaattt
     2761 tcgcctacat gtatgttata tccataatca cacctaatgt tttgttctac atttatttgc
     2821 ttgccaactg aaccaaatag ttgtcttaaa atatcttctc ttttttcata ttcatcctct
     2881 aaagtattat taaacaatct agttaatttt ttacaatact ctctttcttt tactaacaat
     2941 tcatcatttg catagtatcc ttttccagac aacatttttt ctttttcagt catatagttt
     3001 actccttttt gttataattg tatctaaagc aaaataaata ttcctgtgga aaatctaaat
     3061 atattctgtc acctttctta aatctcattt tacttttttc caattctatt tgaataggta
     3121 cacattcgtt agataaatca gtttttctaa catatatagt atatgtaaaa ggattttcta
     3181 caatgttaat caattcgaaa cataactttt catttaaatc ttcattagaa tccaagacct
     3241 taatatgatg ctccctgatt cccacatatt ctatattatc acctatttct ctatttaatg
     3301 tcaactcgca tccccagtct gtagcataaa tagtattttt atccagtcta tttaatttgg
     3361 atatattttt acaccctgtt atcttagctt ctataaggta ctttggagat tcaaagagct
     3421 cttttttagg tctctttgga agaccttctc ctttgttgta cactataata tcatcacaaa
     3481 ctcggtaagc ttcttcaata tcatgagtaa caaatagtat atctcccttg taatctttta
     3541 acatattcat aagttctttt tccatattac ttcttaaatg atggtccagt gctgagaaag
     3601 gttcatctaa aagtaatata tctggagatg ttgccaatgc tctagctagt gctacccttt
     3661 gttgttgacc accagataat tgccaaggat atctcccttc aaacccattt aatttaagta
     3721 tatctaagta ccttgctact atttcctttt tttcactctt acttagtttt aaaagcccaa
     3781 gttctatatt ttgacttaca gtcatatgtg gaaacaaagc atagttttga aataaaaatc
     3841 ctacctttct atcttgtgtt gataaattta tcttcttttc agaatcaaat agaactctat
     3901 catttaatac aattttacct cttgttggtt tctcaagccc tgctatacat tttaatgtca
     3961 tactttttcc agaaccagat tcccccaaaa accccagagt acctttttct tgtttaattt
     4021 ctacctttag tttaaaagag gataaatctt tttcaatatc cacgtataag ctcaactatc
     4081 cacacctctt tcctataatt ttttgctgaa attccgacca ataatttagt aataaaatca
     4141 ttgctataga tattgctaca ataatcataa cccacatcat agctttattc atatctccgc
     4201 cttcaacagc aaaaaatata gctattggca tagtttgagt ttttccaggt atatttccag
     4261 ctatcataag tgtagcacca aattcgccaa gagctctggc aaacgacagc accaatccac
     4321 caattattcc aggccatgct aatggaactg caatcttaaa aaatactttc cattcattaa
     4381 gacctaatgt tctagcagct gacagcatat tattatcaat ctgttcaaaa gctgaccttg
     4441 aagttctata catcatagga aaagacacca ctactgctga aattacagtt gctgtccaac
     4501 taaatattaa agtcctatcg aaatttaata aaaactttcc tataaatcca ttcttcccac
     4561 atagtaataa taaaaagaaa cctacaactg ttggtggcaa aattagtgga agtgtaaata
     4621 gtccatctat aagacccttc cacttacctc tatagttagc cataatatat gacacaaata
     4681 ttcctattac aaaagttatt attgttgaca aaatagatgt cttaagtgat atccataagg
     4741 gtgaccaatc tgttcccatc tttccctcct taattttaaa tggaaatagt gaaactaata
     4801 actctattaa tttcactatt tccattttat tataatacac aattattttc aattttcatt
     4861 tatttatata taatttatac ttaattacac ataatttatt tataagttta ttctactttt
     4921 ttatatccaa attcttcaaa aatcttttgt cctgactcac ttaataaaaa ttcttcaaat
     4981 ttcttagcag catctacgtt tttactagct ttaattactg aaactggata tgtaataggt
     5041 gaatgagttt tttcgtctgt tttttcaact acttttatct tatcattatt gacagtatca
     5101 ctgaagtaaa cgaatccaac atcagcattt cctgattgca cccatgctaa aacttctttt
     5161 acatcttttg caaatactag cttatctttt aacttatctt ttaaatttaa attagttaac
     5221 acctcatctg catacttacc agctggaaca ctttcaactt caccaacagc aattttcttc
     5281 actttatctg tagtcaaatc tttcatacct gatacactac tgtcagcaga agaaataaga
     5341 actaaatcat tttttactaa atctttcata gtatcagaaa ctaataactt ctcttcatcc
     5401 aaagctttca tttgtttttg acctgctgaa ataaataaat cacaaggtgc tccttgttct
     5461 atttgttgtt gtaatgagcc tgatgctcca tagttaactg taagttttac atttgaatca
     5521 acttttttat attcttcttc aatctttgcc attgcttctt ttaaacttgc agcagccgat
     5581 atatttaact ctactgaatc actactttta gttgtttttt cttgttttcc atcatctgtt
     5641 ttgtcttcat tactattgca cccaactgtc cccaaagtta aacatgcaac taatcctaat
     5701 attcccaata tttttttcat aacaatctcc ctttttgtaa atttgataca cattttaata
     5761 tgttttatta tgttttatca tgttttgtta tatatagttt aattatatat acatgataat
     5821 aaattttcaa gtaatttatt tacactactt ttaaatattt tgctacattt tccttttttt
     5881 aatgttaaaa taaccttata tggaggtgga ttatggactc attatcatct ttgacacctg
     5941 tcgaggtagc tgaattatta aagataacaa aaaatactgt atatgaatta atcaaaagag
     6001 gtgaacttcc ttcttataaa gtagggaaaa aaataagagt tgatactaaa gatgttgaag
     6061 aatatatcaa cagccaaaaa actggtaaag taaaggatac acatttcaac aataaaaata
     6121 tagatataaa taataatttc aaaattatta tttctggtca agatgtaata ttagacattt
     6181 tagctaggtc tgttgaaaaa aaattggatg gaataagtac atttcgctct tatataggta
     6241 gttataatgc tttgtatgag ttatataatg gcagagtttc tatagcatca tcccatctat
     6301 gggatttcga aacggatgaa tataatagca cttttgttaa aaagctattg ccaggaatac
     6361 catgtgtact tataaatcta gcttatagaa tgcaaggttt ctatgttgct aaaggaaatc
     6421 ctaaagagat aacaacctgg gaggatttaa caaaaccaag tataaccatt gtaaataggg
     6481 aaaaaggttc tggaactagg gttttactag atggtaaact tagacttcta aactttaatg
     6541 gtaaacatat aaaaggttat gataatgaag aattatcaca tcttggagtt gcaagtactg
     6601 tttctcgtgg aatagctgat gttggtttgg gaaatgaaaa agctgcttta caagtaaata
     6661 atatcgactt tattcctctt cacaaagagc gctatgattt agttattaaa aaagaagact
     6721 tacaaaatcc agtttaccaa actataatta acataataaa ctctcctgag tttaaagctg
     6781 aacttcaagg tcttggcgga tatgatttaa ctgatactgg aaaaactata gcaaaagttt
     6841 aagattaaaa tcgcaatcta ttttataaga tttataattt ttcacaaaat ttataactta
     6901 aacacgaaat aaattgcgat tttagtgtct caaaaaattt atgtctttca ttttatcatc
     6961 caatacaaaa tataatataa ttaattgtgc aatcatatta tattaatata tatttacata
     7021 atagtatttt tctttaatgt agattaacat atgatatgtt ttaaacatca acacccttgc
     7081 tttcttatat atatgtggca acacatatga gtttaataaa ttatatacaa tgttgctttc
     7141 ttcttgtatt ttttcaacca acaagttatt tataaaatta cctttctcat tttcaataat
     7201 ctcactccaa tttatcttaa cttttaatat atatcttccc actaggtata cacactctac
     7261 aataaataat aaaaatacaa tacagtcagt acctgttgtc ttatataaat tacccgatac
     7321 taatggcttt gcgaagaaat tataaaaatt caaagtatca cccgtataat caaaaagtat
     7381 taatagcact cctagaactc tcaatacttt aatattcaac atgtttttat ctttattatt
     7441 tttgtttttc atatatacta cctctctttt ctttagattt gattgtaatc taatactaac
     7501 tcttttattt gaggtataaa tgaatttaat tggtagttat tttcattttt actagaaatt
     7561 aattcacatt actaaacaaa acttatattt aaacataaaa atagcctatc tattttttaa
     7621 taaatacaaa aataaataag ctactaatat tcaattaact tttatcactt aaaacattac
     7681 tatctataaa tttaaatatt ttattgaagt atactgtact atcatgttca aatccatcaa
     7741 catgaccaat accttctata agccataggt cttttttatc atgtttaact aaatcataca
     7801 ttttcttgct attttgataa ttacaaacag tatcttttgt tccatgtata aacagtatgg
     7861 gtacattaga tttctcaact gactcatatg gttttacatc actgtaccaa aatccagatt
     7921 taaacttatt atataaatct ccacaaatca ccacataatt gattaatata tctggtattc
     7981 gtttttctag tactcccatt ctaattgcat ctttcatttc agaataagga gaatccagta
     8041 tataaaaatc gaccttatca tcttttgaat ttatctctga atgcatagca gctgtccctg
     8101 ctcccatgga aaatccatgc actcctaatc ttccttcagg aaatttattt tttacaaatt
     8161 taactagact atctaaatca tacctttcat aaagaccaaa tgtgtaatta tctccaccac
     8221 tattaccagt atttctttga ttataaacta atacattata acctttatct aaatatctat
     8281 atgctacctt taacatttca tagtaactac ttccaattcc atgaacaagt atcatagtat
     8341 ctcttgtctt tatatttgaa gttataaaaa gactttcaag cttgtaatta ttagaacttt
     8401 gtatcattaa ttcagatgtc ttgtagtttt ttaatttctc cagtggttta tcttttctct
     8461 cagaaaatat ttttactata tcttcgtttt ttattaactg ctctaaacca accgaactat
     8521 tatacactaa tttgccaaat atataagata caattaatag gattaataga aacacaattg
     8581 atataattat aaatttattt cttaaactaa tacaattttt aaaactattt ttcatattta
     8641 tttaccatac aattttcct
//
LOCUS       DAEPOZ010000156         8605 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.156, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000156 DAEPOZ010000000
VERSION     DAEPOZ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8605
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.156"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(139..2418)
                     /locus_tag="KR440_002656"
     CDS             complement(139..2418)
                     /locus_tag="KR440_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0699691.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITEKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYIARSNGNIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFAQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(2689..4143)
                     /locus_tag="KR440_002657"
     CDS             complement(2689..4143)
                     /locus_tag="KR440_002657"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBH0699692.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYNSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVEIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            4307..5176
                     /locus_tag="KR440_002658"
     CDS             4307..5176
                     /locus_tag="KR440_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH0699693.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDATSDLEK
                     CFKIATENKCNRLRLNIHDIESIKSFIEQINISESNTEFGNEILRNALFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGYLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
     gene            complement(5390..7462)
                     /locus_tag="KR440_002659"
     CDS             complement(5390..7462)
                     /locus_tag="KR440_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH0699694.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLSHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYASIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKLLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENALVEKIDSLTNGEILDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSKKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNCLSTISPVVFTNLEQNQKLSIKKGITTAKEENRVSIL
                     RYDIEKIG"
     gene            complement(7765..>8605)
                     /locus_tag="KR440_002660"
     CDS             complement(7765..>8605)
                     /locus_tag="KR440_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0699695.1"
                     /translation="IIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSKI
                     ERTFVIQDESFLDKISKLASQVDEKFFEISTEIIAYAEENLNTKLYEYIYVALTDHIA
                     FAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMHI
                     VNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVKK
                     EKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVISV
                     LNL"
BASE COUNT         2906 a         1412 c          883 g         3404 t
ORIGIN      
        1 tttgcccaat aattcctaaa ataatatcct aacaatgatt tatgttttta taatagtata
       61 tttttacata aaaaagtgaa cattgattaa caaagttcac ttttttataa atttttatgt
      121 tataattcac aattttattt aatttgtttc ttttctattt aaactatatc tatctaatat
      181 taatttttca tattcttcta ttggcattgg tctagcaaat aaaaatcctt gggccatatc
      241 acaacctata aaccttaaaa actctgcttg ctctacagtt tcaacacctt ctgaaacaac
      301 ttttatatca agttgctttg ccatttgaac aatacttgaa actacaattt gactcttttt
      361 tgcatttccc actgttataa agaattgcct atctaatttc aatacatcaa ttggcatatc
      421 tttaagcata tttaaagaag aataaccaga accaaaatca tccatagaaa ttttaaatcc
      481 aacagatttc aaagtttgca ttattttaaa taatattttt gtattgtcaa atattgcact
      541 ttctgtcaat tcaatctcaa ttaaatttgg ttcaatttca tattcttttg ttattctttc
      601 aagctcagaa ataaaacctg gattctcaag atgtactctt gataagttta ttgatatagg
      661 aaatgtgggg aaattttcat tttcccaacg tttaaaattt ttacaaactt gggcaaatac
      721 aaacatatcc aattctgtta taaaaccatt tttctcaaaa ataggaataa atacacctgg
      781 acttattaga ccttttttgg gatgttgcca acgtacaagc gcttcagcac ctacaatttt
      841 agtagttgat aaatcaacct taggctgtaa ataaacttta aattgttcat tttttaaagc
      901 atcatgcatt tcttgttcaa tatcacgttc ttctatgaga gtttctttta aattttcagc
      961 ataaatctca taaactttta aatattttct ttttactgta gtttttgcaa tcatagcttt
     1021 gtcaagacag gtagaaatat ttgtttctcc ctcttcaaca aaatatacac ctatggatgg
     1081 aattaaattg agttctactc ccaaacttgt ttttatcatt gacaattttt tacgtataag
     1141 ttcacatatt tctataattg actctctatt atttttcttt tcaaatatta tagcaaaatt
     1201 gtcatttgaa attcttgaaa atacagattg ctctttaaat atattcttaa ttatctctgc
     1261 tatttccttt aatactctat ctccttcttc atacccaaac ctatcattta ctagcttaaa
     1321 cttatctata tcaaaataaa ttaaaactag attctttttc tcatgagagg ataaaaattt
     1381 tcctccttca agattaaatt tatttgaatt gccaatacct gtaacgttat ctttaaaagc
     1441 aaattcttcc aatttttcct tactcttttt atgtataaat agtatataaa ttgttaatgc
     1501 cgtaaagatt ataatcaaca ctacacatgc tgataatgta agttttataa tagcgtatga
     1561 tttctcagat atagcagttt taggaacttc agaaaaaaga taccaatcat tgattcctat
     1621 tggggcataa cttaaataat atccttttcc aaacatacta taacttaatg aacctgattt
     1681 aaagtttttt atatttgact tcatattatt gatactagat tttgatattg aatcacttct
     1741 gccaatgtaa gaaaataaat tttctttagt agagttggcg tttttgctgc ttggatttaa
     1801 aacaatatta ccattacttc tagctatata agaatatcct tttccattaa atgtaggtac
     1861 agaaagtatt ttttcataca acttcgtact atgtgtggca aatataactc ctttaacctc
     1921 attatcttta taaattggca cagaatatac attaatatta ctattggtat ttttatcatc
     1981 ttctattaca tcttttaatt tttctgttat aaccacttct ccttgcattg attttaagaa
     2041 ataatctctt ttagagaagt cttctatata accatctgta gtgtatgctg taccatttgg
     2101 taaaataact cccattcttt taaatgaact attaatagct tgtcttttta gtatagaaag
     2161 gatattatct acttcaaaag actcttctcc ctcaataaat attgaaattg actctagtaa
     2221 ctttatatct ccattaattt gtttttttaa cacattgacg ctttgatttg ctatttctga
     2281 aagatattga ttcgtttctt cacctaatgt cttattcaac cttgaaccat aaatcaaaca
     2341 ggaaattgta attactaaga tacttatgac agcaattatg gacaaaccat acctattatt
     2401 ttttgcatca cttaacatta tgcttcctgc ctttctcgaa tctatgtatg tataatcatt
     2461 atcttatgac aattattgta aacattttat aataatactt atttttccat atttactgta
     2521 attatacaaa attataaact ttattacaac aataaattaa ttaatataga tattagggtt
     2581 tatattattg cattatctat tttacaatca tttacatata aaaaaatgta atatcaaagt
     2641 gaaacttgat attacatttt tgttcttgta ttttgtttaa tttttatgct acattttact
     2701 taaacactca tttgcaactt ctttatcatc aaaatgatgc ttaactttac ctataatttg
     2761 ataagtttca tgtcccttac ctgttataat tacaacatca tccttttttg ccatacttat
     2821 agcttctgct atagcttgtt ctctatcaac aacaacatgg taattttctt ttttcttgtc
     2881 aattccttca agtatatctt ttattattgc ctctggttct tcagttcttg gattatcaga
     2941 agttataata cataaatcag aatatttttg tccaattgct cccatcaaag gtctcttttc
     3001 agtatctcta tctccaccac atccaaacac agaaattatt tttccttctg caaattgttg
     3061 agttgtcttt agtacatttt caagtgcatc aggagtatga gcatagtcta caattacact
     3121 tataccttta tcatttggaa ctgtttcaaa ccttcctgat actccattag aaagtctaag
     3181 accttcttta tatattggtt taggtatgcc aagtacatag caagcagcta taactgctaa
     3241 agtattatat actgtaaaca ttcctggaac tggtataaat attacttctt catatgaagg
     3301 tgttattaat ctataagata ctccatccga atctgatttt atatctctag ccataaaatc
     3361 agccttagta tcaacaccat aagtgtaaca aggtacattt atacctttta tattttcata
     3421 tatctttttt ccaccttcat catctatgtt tattatattt gccatagtag tcttaaaaaa
     3481 taatttttct tttgcttttc tataatcctc taaatcttta tgaaaatcta aatggtctgg
     3541 tgttaaattt gtaaatatgc ctagtttaaa gtctgtttca tcaactctat ttaatgccaa
     3601 tgaatgtgat gaaacttcca tcgcacagta gtcacatcca ttatccaaca tattattaaa
     3661 atgatattgt aaatctatac tttctggagt tgttgaattg gaaacaatgt ctttttctcc
     3721 gtcaaaaatt tttatagttc ctataagtcc aactttattc ttatttaaag ttaatatctc
     3781 atttagtata gtagttatac tagtttttcc attagtacct gtaactccta taacgttaaa
     3841 tttctgagat ggatgattat aaaaattatc agctactttt gccatatctt ttcttgtatc
     3901 ttttaccttt ataaatgtac atccttttat ttctacatct tcttcaacaa gaaatgcact
     3961 tgctcccttt tctatggcat cttttatata cttatgacca tctgacacaa atccttttat
     4021 acatataaat aatgtacctt ttgtaacctt tcttgaatta tattgtacat tattaatatc
     4081 tatatttaat tcacccttga cattgattat atccaacccc tgtataatat catttaattt
     4141 cataaatgct ttcctccctt taaactcttt ctgctaaaat tacactacat ataatattat
     4201 attaagaatt ttttactatg tgaatggtta aaaatgcttg aaatattgca aaaaatgctc
     4261 tataattttc tataatataa ctgaggaaag gtcttctata aaaactatgg ataataaaat
     4321 cgggtactta aacgataact ttaaaatatt taatattaga gataaaaaag atatacaatt
     4381 tgaataccat aatcatgatt ttaataagat aattatattt ataaatggaa atgtaactta
     4441 ttttgttgat ggtattccct ataaactaaa gccttgggat atactcttta tcagcaataa
     4501 tgaaatccat aaaccagtta tagattcaaa tgtcttttat gagagaattg caatatggat
     4561 tagcccagaa tttatgaaaa aaaatagtga tgctactagt gacttagaaa aatgttttaa
     4621 aatagctacg gagaataaat gcaatagatt gcgtttaaat atacatgata ttgaatctat
     4681 taagagtttt attgagcaaa taaatatttc cgaatccaat actgaatttg gaaatgaaat
     4741 attgagaaat gcattattta tacaacttat gatattctta aacagaattt tcctaaaaaa
     4801 tgaaaatatg gaatcaacag aagacgtttt ttatgatgaa actattaaga aagtattaaa
     4861 ctatataaat tcaaatatag atgataagct ttcaatagat agcatatcgt ctgaattttt
     4921 tataagtaag tattatctta tgagaaaatt taaatctcat actggaacgt ctatacacaa
     4981 ttatatagtt caaaaaagat taattttagc aaagtcacta ataaccaatg gttaccttat
     5041 gagtgaagtt tgtaataaat gtggattcaa cgattattct agttttgtaa gggcatttaa
     5101 aaaaaactac ggtgtatctc ctaaaaatta tataaacaat aaaaatcctt tagatattac
     5161 tcttttagat gaatagataa tttatgtaaa ttttatttta ataaaaactt gaagtattag
     5221 tgtatttatc ttactaatac ctcaagtttt ttaatgtttt aaaatatatc aaaatataat
     5281 gatatatatt tctattactt aaaatatata ttaaataaat tgcatgttaa ctgaacctat
     5341 ttatatattt attaaatctc acataacttt tagttatact atcatatagc tatcctattt
     5401 tttcaatatc atatcttaat atacttactc tgttctcctc ttttgctgta gttattcctt
     5461 ttttaatgct taatttctga ttttgttcta aattcgtaaa tactacagga cttattgttg
     5521 atagacaatt tttttcaata tattctaagt ccattttaac taatttttct cctgctttta
     5581 cttcatctcc tacattcaca aatacttcaa atccatttcc ttctaaattt acagtttcta
     5641 tccctaaatg tataagtatt tctactccac tttttttgga ttttattatt attgcatgct
     5701 tagttggaaa tataactcca atagttccat caactggtga ataaatcaat ccatcactag
     5761 atttaattgc aaatccatcc cccatcagtt tacttgaaaa tacagaatct ggcacttctt
     5821 ctatatctaa tatctctcca tttgttaatg aatcaatctt ttctactaaa gcattttcca
     5881 tattggcaac ttccatattt tttttcactt tatttttatc aacattttta gtaacagtac
     5941 tttcaacctt tgtttttccc tctattatat cttttatatc agactttatt gaatccgatt
     6001 ttggtccaaa tatagcttga acattgttgc caataaccat aacacctgct gctccaagta
     6061 atttcaattt ttctttatct actttattta tatcatttac aaccaatcta agtcttgtta
     6121 tacacgcatc taggctcttt aagttgtctt tatttcctaa agcaattaaa atctctgatg
     6181 ctaattcatt cttagacatt tcaatgactt cagcgttagt ttctgactca tcttctcttc
     6241 ctggtgtctt taaattaaat tttcttatca taaatctaaa tacactatag tataatactg
     6301 caaaaccaat acctaccaag atagccaagt accatggagt tctattagga agaacaccaa
     6361 ataataagaa atctattaat cctcctgaaa atgtgaatcc aattttaact cctaataatt
     6421 gcataattgc gtatgataaa cctgaaaata aacaatgtat tgcaaataat actggtgcaa
     6481 caaataaaaa cgcaaactct attggctcgg ttattcctgt taaaaaagag gttaatgctg
     6541 ctgatgccat tatacctgct acaaattttt tcttttctgg ctttgcttca tgcaccatag
     6601 caagagctgc tgctggaaga ccaaacatca taaatggaaa tgcacctgtc ataaaagtac
     6661 ctgctgtgaa atttactcca tctttaagtt gagcaaagaa tatcttttgg tcacccatta
     6721 caagctcacc agctttattt acatattctc caaattgata ccaaaatgga ttatggaaaa
     6781 catgatgcaa accaaatggt attaatagtc tttccaaaga accttccata aatgctgcta
     6841 gtgtttgatt tgcttctata acattttttg agaaactaag taatccctct tgtattggag
     6901 gccaaataaa agacaatata attcctattg ctatagcaac taatgaagta acgattggta
     6961 caaatctttt acctgcaaaa aaacctagat actgaggtaa ttcaatttta taaaactttt
     7021 tataaagata agcagccact atccctataa taataccacc aaatacccct gtttgaattg
     7081 ttggtattcc aacaatactt gcatacatag aattgtctac catatcttct gtaacacccg
     7141 ccataacacc catagatgta ttgagaatta aaaaacctac tatggctgcc aatgctgcaa
     7201 caccttctcc ttcagttaaa ccaacagcta ccccaactgc aaatataagt ggtaagttat
     7261 taaatataat ttgacctgaa ctttccatta tacttattat aagttgaaat gtaggattcg
     7321 ccaaagctgg aaaatgagac aacaatgcgt ctcctgctaa agcattactc acgccaagta
     7381 atatacctgc tgcaggaagt aatgcaactg gtagcattag agattttcct actttttgta
     7441 aaacaccaaa tacttttttc atttactttt ctctccttat tattttatat ttttttaaat
     7501 aaaaaagact taagtttata aaaagttaca ttagtatact taattctaaa attaaactta
     7561 tgttaatata aataaaccta tattaaccta atttcagaat taaaaataaa ctattataat
     7621 ttttttataa acataagtct tgcctgcatt atcagtaaca atcttaatgt tttaaaatta
     7681 ttattttata ttaaatttta aataatatat taaaattaaa cataatatat taaacctata
     7741 cattctgtat taaactactc tttattataa attcagtaca gatataactc ttttgatatg
     7801 taatgttaaa taaagtagtt catcttgact tacatagtaa tcataattct tttctacatg
     7861 agtttttatt ttatatgcac aatcataatc cttttcatac tttactttaa ttatatctat
     7921 aatttcatta tttgtatcat tgattttttc tttttttact aatcgttttg caaagaattt
     7981 taaatgagtc aaaagcctat cataatttat atcatcttct ttaaattcaa ctctataata
     8041 atatcttata atatttagta tgtcttttac aattttagta attaacagtg attcctttga
     8101 acttccctta tagtttgaat ttactatatg cattgctata aaaccagctt catctactgg
     8161 aaatttcaca tcaaattctt tatttatata atctacagcc catttaccaa tctcatattc
     8221 ttttttatgt attcttttta tctcatgtag taaatcattc tttatagtta tattttcatg
     8281 atatctttta attgcaaatg caatatggtc agtaagtgct acataaatat attcataaag
     8341 cttagtattc aaattctcct ctgcataagc aattatttca gtactaatct caaaaaattt
     8401 ctcatcaacc tggcttgcta gtttgctaat tttatctaaa aagctttcat cttgtattac
     8461 aaaagttctt tcaattttac ttttatctat ttcttgacct ttcttttttt gaaacccaat
     8521 cccacatcct gttagtatta attctttttt tatattttca tctaaaacta atacaacatt
     8581 attattataa atttgttgaa taatc
//
LOCUS       DAEPOZ010000157         8599 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.157, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000157 DAEPOZ010000000
VERSION     DAEPOZ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8599
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.157"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            55..840
                     /locus_tag="KR440_002661"
     CDS             55..840
                     /locus_tag="KR440_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HBH0699696.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDGLEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            1132..2451
                     /locus_tag="KR440_002662"
     CDS             1132..2451
                     /locus_tag="KR440_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBH0699697.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMSIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(2526..2954)
                     /locus_tag="KR440_002663"
     CDS             complement(2526..2954)
                     /locus_tag="KR440_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699698.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(3045..4421)
                     /gene="hydG"
                     /locus_tag="KR440_002664"
     CDS             complement(3045..4421)
                     /gene="hydG"
                     /locus_tag="KR440_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HBH0699699.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(4422..5507)
                     /gene="hydE"
                     /locus_tag="KR440_002665"
     CDS             complement(4422..5507)
                     /gene="hydE"
                     /locus_tag="KR440_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HBH0699700.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSPDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNKDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(5543..6751)
                     /gene="hydF"
                     /locus_tag="KR440_002666"
     CDS             complement(5543..6751)
                     /gene="hydF"
                     /locus_tag="KR440_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HBH0699701.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVNKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGILIAYVKGI
                     LERSLELFNY"
     gene            complement(6766..7017)
                     /locus_tag="KR440_002667"
     CDS             complement(6766..7017)
                     /locus_tag="KR440_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HBH0699702.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
     gene            complement(7398..8438)
                     /locus_tag="KR440_002668"
     CDS             complement(7398..8438)
                     /locus_tag="KR440_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBH0699703.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLGLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT         2990 a         1254 c         1211 g         3144 t
ORIGIN      
        1 tatttttacg ttgtgcataa aataagaaca ttagtgaagg agtagagata aattatggag
       61 ataataaaat atatcttgta cttttttata tattcatttt taggatggac agtagaaagt
      121 ataggatgtt ctatagcaag caaaagaata atcaacagag gttttttaaa tggtccaata
      181 tgccctgttt atggttttgg agcagtaatt gtgatatcac tattaggaag atttaataat
      241 gtagtaatag tatttctatt aggaatgatt ttaactacga tactagaata ttttacagga
      301 tttattttag aaaccttatt tcatgctaaa tggtgggatt attctgacag aaaattcaat
      361 ataaagggta gagtatgttt aaaaaatgca atatactttg gtgtaatgtc tgttttaata
      421 ataagattta tacatccatt tataaaatat tttgtaagta taattccata tagaatatta
      481 atttcaatgg ctataattac aacactttgg actatacttg atttgattgt tactattata
      541 acacttaaaa aattagatat aaagttaaac ttactagacg atataattac agaccttaat
      601 gatataaatg tgaaattgga taaatttgat agaggagaaa tacaagcttt atttaaaaca
      661 ataaataacg acggattaga agttagagaa aaaataaaca aaataaatag taagttggac
      721 cgtatagagt ctaatattat tttacaaaaa agagttataa aagcatttcc acatataaaa
      781 cataaaaaac aacaagagca actagaacat tttaaaaaaa ttattaatga gaaaaggtaa
      841 ttatacaatt taatgaagac gaatagaata atggtattta taagattatt ttcagaggac
      901 tctataaaaa tagaatagag tcctttattt tttgaaaaat aatgtttatt tgataaaaat
      961 aaaatatatt gtttctaaat aacaaatatt ttgaaaatta taaaaacata tattgacaat
     1021 ttgtgtcaaa aatactaaca ttaagtatat aaagaatttt atatgagaat atctttataa
     1081 atcgcaataa ttttagaaca taaatttatt taaagagggg atgatttgcc aatgagcgag
     1141 ataactagtc aaatgataag tacagaagaa aaaaaatatg ttgctaaaac tcaaaagata
     1201 ccttattatc cagtagcatt taaatcaggt gatggtgcaa tgctatatga ttatgaaggt
     1261 aatgaatatg tagatttttt agcaagtgca ggctctgcaa atgtaggtca tggtaataag
     1321 gaaatctcac aagcagttaa agaacaaatg gatgatataa cacaatatac acttgcatat
     1381 ttccatagtg atcctcctgt aaaattagct gagaagcttg tggaaatagc ccctggagat
     1441 aatgataaaa aagtactata cagtgcaact ggttctgctt gtatagatgc tgctataaaa
     1501 ttagcaagag ggtatactgg aagaacaaag ataatatcta tgtgtgaatc ttatcatgga
     1561 agtacttatg gagctatatc tatatctgct ttaagtacaa atatgagaag aaaaatgggt
     1621 ccattgttac cagaagttta tcacttccat tatcctgata aaaatagaac agctaaagaa
     1681 tgtttagatg agatagaata tgcttttgca cactatctac cagcagaaga ggtagctgca
     1741 atatttatag agccaattgc aggagatgca ggaataatag tgcctccagt agagtgggta
     1801 caaggtttaa gtaaaatatg taaagaaaat ggaatattac ttgtaagtga tgaaatacaa
     1861 caaggtatgg gaagaacagg taagtggttt ggtatagaaa attttggtgt tgaagcagac
     1921 cttatagttc ttggaaaatc tgttggaggt ggattacctt taggagcagt tgttggaaga
     1981 acagaaataa tgcaaagtct tgatgcacca gcacatttat ttactttagc tggaaataca
     2041 acagtgtgtg tcgctgcttt aaaatctata gagataattg aaaaagaaaa cttacttcaa
     2101 aagagtatag aaatgggaga ttatattaaa gcaggatttg aaaaactaaa agaaaaatac
     2161 gatataattg gagaaataag aggaataggg ttatctatag gagttgatat agttaaagga
     2221 aaaggttcta atgaaaaaca tccagatgct acagctaaga tatgttatag atgtatacaa
     2281 actggattaa taatgatatt cttaggacaa tctactttaa gagtacaacc acctctagta
     2341 ataactaagg agcaagtaga taaagcaatg agcatcatag attctgcaat agatgattat
     2401 ttaaatggta gaattggtga tgaagtatat gaagtaactc aaggttggta aaataaatat
     2461 taaatgaaaa aaagagttat ctcaatttta gaaagtaatt tctgaaatga gataactctt
     2521 ttaagttacc cacaaataag aaaattgttt gattcattaa agactatatt tcggatgtcg
     2581 tttttgtgag tatcagagac agtattacga gaataattac ctttattcgt actatctgtt
     2641 aatttagtta taattaagat agctaaaaga ataagaatta ctgtgtataa agcaataaca
     2701 tatggttttc ttttatattt gtatttgtta ttgaaaaaag aatggccata attattttta
     2761 tttactttag atgtatttgt attctttttc ttgttacctt tctttttatc atcctttttt
     2821 ctatttttag ggtaaggaat gactttgtgt gtattatcaa gtttgatatt tttatttttt
     2881 tgaatattgt tttccaggct agcctttttt tctccaaatt gaatagtttt attggtattg
     2941 ttgttatctt tcaatgttaa tctccctccc ttaaactttc cctttagata atattactac
     3001 taacctatgg aatatacaat aaataatata taagtaataa aattttatag gtataaatct
     3061 ctttctccac gctctaaggc agtcatattg ttagatacta tgtttcttat atcttctctt
     3121 tctatcttac ctatttcttc tgcgataagt tttttaccca taattttaag ttcttcatct
     3181 ccataatcga ttaaaaattc atttaatgta gtaagagcat ttggcgtaca tacattatgg
     3241 atttgtcgac ttttagctaa gctcatgaat ctatcgccag ttctaccttt acgataacaa
     3301 gctgtacaat aactaggtat atatccatca gtaatcagtt ctttaagcac ctcaacaggg
     3361 cttctatggt ctccaacttc gaattgttct gtattgtcac catcttcata tgctttatat
     3421 cctccaacac ctgttagaga acctgcacta atttgtgata caccatattt tagtaactca
     3481 tttctcattt cagcagtttc tctagtggac attatgattc cagtaaaagg aacagccaat
     3541 ctagttatag ctactatttt tttgaacata tcgtcagaaa ctaagtgagg aaaatcttct
     3601 aagctcatac cttcagcttt tttcaatcta gggaaagata tagtatggaa acctacacca
     3661 aatttttcct ctaagtgttc gttgtgcatc ataagtccta gaacttcaaa tttagggtca
     3721 gataaaccaa ataaaactcc tgcaccaaca tcatcaacac cagcttccat agctctatca
     3781 aaagctgtta aatggtaata atagtcattt tttatagatt gaccatgcat ttttataaat
     3841 gttggtttgt gataagtttc ttggaataat atataagttc ctattccttt ttcttttagt
     3901 aatttatact catctacact tgtagctgct atgttaacgt ttacccttct tatatttccg
     3961 ttttcattat atgttgaata aattgcatct aagcaatcta agatgtaatt tatatcacaa
     4021 tttttagggt cttcaccagc ttctagagca agtcttttat gacccatttt ttctaatatt
     4081 ttaacctctt cctttatctc ttccatggta agtttttttc ttttaaattt gttgcattta
     4141 ctaaaaccgc aataaacaca ttcatttaca cagtagtttg atacataaag aggggcaaat
     4201 aaaaccactc tattaccata tatttcattt tttatttgcc cagctatact aaataatcta
     4261 tctagttgtt ttttatcttc tacttcaaga agtgtagcta tatctttata actaagtttt
     4321 tcccttctat cagccttatc aagtaccttt tcaatatcat cagaagttga attttttgca
     4381 tcttctaata aagaattaat aagttcatgg tttatgaaca tttacaaacg cctccattca
     4441 atattatcac cgcgagaata gtcaacttca agtcctaatg acttgatgtt ttcttctaaa
     4501 ctttggtgat attcacaagc ttcttgacca gtaaaggctt tgttatcgta aagagaatat
     4561 tgttttctaa attcttgagg agaaaggttt ggcataatta cattgcaacc agctaaaaga
     4621 cctgcatttc taccactagg gtttatagat gccaaggctg ttgtagcggg taataaaact
     4681 tttggaagta gaagtcttgt aattgaaagc ataagtaatg tcttctctaa atctccatgt
     4741 ttataatcct taaaaattgt atcatggtgt ggaataaaag gtcctattcc aaccatatga
     4801 ggatttaact cttttaggta taataaatca cgaacaaggt ctttgttgtt ctgaaatggt
     4861 gattcaacca taaatccagc acctacctga taaccaattt ctttaaggtt ttttaggcat
     4921 tcttttctag ttctaagctc aatgtttggc ggatgtaatt ttttataatg actgtctgta
     4981 gcagtttcat gtcttaaaag atatctatct gcaccacata aaaagtattt tttatacgaa
     5041 tcataactct tttctcctag tgacagagtt atggcacaat cctcatattt attttttata
     5101 cttgaaacaa tttcacatat tttatcatca gtaaaatatg catcttcacc accttgcaaa
     5161 acgaatgttc tgtatcctat tttataacct atatcacaac attcaagtat ttcatcaggt
     5221 gaaagtctgt atcttatgat atttttatta gaagattgta ttccacaata gtagcaatta
     5281 tttttacaat agtttgtaag ctctataagt cctctcaagt agactctatt tccataatgt
     5341 ttatttctca tgtcgctagc ttgtttataa agatagtttc ttaaattttg ttcgaagtta
     5401 tattcctcat tgtcgatatt ttctattaaa tatagtagtt catcaaaact taatgaatta
     5461 atgctgtaaa gtttatctat aaagtattta atcttatttt tagtcatatt caaatatgta
     5521 attattaagt atagttaaat ttttaataat tgaaaagttc aagtgatctt tccaaaatac
     5581 cttttacata tgctattaag ataccatagt ttacaatagg tacattgaaa gattttgcct
     5641 tttctattct agataacatt ccagctctat tcatcataca agctccacag tgaactacaa
     5701 gggcatattt atttatatct tcagtgaagg atactccaga actaaattca aaagtaatat
     5761 ttttgccagt tttctttcta atcatattag gaatttttac tgttccaata tcatcagttt
     5821 gtctatgatg ggtacatcct tcagccatca agattttatc accatctttt aaattttcta
     5881 aagcataagc tccattaatc aattctttaa ggtctccctt ttgtctagca aacaatatag
     5941 aaaaagatgt aagaggaata tcttttggtg tatctttatt tacttgtgga aatacttgag
     6001 agtcagtaat aacaagtttt ggttttttgc ctaaattaga tagagtttct tttaaactat
     6061 cttcttttgt aactatagaa attgcaccat tatccaaaat atctcttata acttgctgtt
     6121 gaggaagtat aagtctacct tttggagctg ctttatctat tggaacaact aaaacaacta
     6181 aatcatttgg ttcaattaaa tctgatacca atttgaactc agtgctatcc ttaggcaaaa
     6241 cttttattat ttcatttttt aaattttcaa ttccaatttt atctgttgat gagacagaaa
     6301 caactggaca tttaacttta tttttagtta agtttaaaat ttctgattga ttcttaatag
     6361 tatcaatttt gttgagaatt agaatatgag ggatattctt atcattaaac ttttcaatta
     6421 gtgacaaatc ttcttgtgat ataccaattt ggcaatccac tactagtaaa gctatatctg
     6481 tcttttctaa aacatctagt gatttaccta ttctaagttc accaagttca ccaacatcat
     6541 caagaccagc agtatctata agaacacaag gtcctatagg aaggatttcc attggtctaa
     6601 atactgggtc tgtagtagtt ccagcaatat ctgagactat tgcagcactt tgatttgtta
     6661 tagcatttat tatgctagat ttacctgcat ttcttttacc aaagagacct atatgtactc
     6721 ttaccgattg tggagtagaa tttagactca tttgtactca ccaccttaaa cttctttttt
     6781 tgatatacta gtttttacag atacatgagg tatgttgccc aatttaccag tcaaactatt
     6841 aatagtatcc atttcgccaa caactgctat acatacgatt gagacacctt cttcttcgca
     6901 aggaatgccc attcttcctt taataatacc tttaaaacct gatacaacct cattgaattt
     6961 aaattgacat tcttttggct cttccaaaat agcacttata actgcaacct ttttcataaa
     7021 tatcctccta actcagaaaa tatgtcttaa acaaatccaa aatcaaaaaa cttttctttg
     7081 taaagaaaag tttctacaaa agaaaagtta ttaattacac aagtataatg tgctatatta
     7141 aactatttcc ttacagatta gataactctt ttctggtagg gttagcttgt aaaaatttaa
     7201 tttaacaaca agtaactgaa gaaaattttt atacaattta tataaattat aataaaaatt
     7261 aactaaagaa aattgaagtt acttatataa tagtagtaaa ttgaaattaa gtctatagaa
     7321 aaaccaattt ttgtataaaa ataaaaatta aagttaatta tttgttaaga attgactttg
     7381 gtaaaatata taattaatta ttctttaagc atataattta taagtaattt tagtgtattt
     7441 tcaatagctt ctatatgtgt tctttcataa tggtgactat tgtttgttcc aggaccaaca
     7501 catgctattt gaacatcttc tccccaacga atagcttctg aagcatctga accatagaaa
     7561 gtaaatacat cgacagcgta gtctaaatta ttgtcttctg caacatcgac aagcttgttt
     7621 cttaaattaa aatcatataa tgataaatta tcttttgcaa ctatagttac accatgttca
     7681 ctagatgttt gacttggtcc agtaggtgct atatctattg ctacaaattc ttttgttttt
     7741 tcaggtaaac atttagaaac accatgtcca atttcttcat aattacttat ataaaagtta
     7801 gtagtatgct taggcgttaa attgttttct ttaaaatatc tacatatttc aagaaccatt
     7861 gctacagctg atttattatc tatatatcta gattttataa aaccagattc tgtagcctca
     7921 aatctatggt ctaggcatac aaaatcccca acacttattc ctaaatctaa tacatcttgc
     7981 tttgtaaata ctttttcatc aagtctaata atcattgttt cttcatttct ttcagtggca
     8041 gcttttgttt gaccatatat atgagtagaa gcatacttaa atacaacact tcctctgatt
     8101 tttttacctt ttctagttat aacagtgcag ttttctccct ctatagctgc ccaacatcca
     8161 ccaccaactt tatggtattt aagagtacca tcagatgata tttcttttac tatagctcca
     8221 agtgtatcca tatgtgcaga tattgttatt tgatttgaat catcttcacc aggaagagta
     8281 gctataagtg cacctttttt tgtaagaata gtgtcaagac ctaaattttc aaaatctttt
     8341 ttgatttcta aaactgcatt ttcagtatat ccagctgggc ttggaatgtc caaatatttt
     8401 ttcattaaat ttatagtatt agttaaattt aaattcatat tgtttcctcc taaataaata
     8461 tatttataca aattataaca taaatacaaa tgttctgaaa actaaaattg aaagaataga
     8521 atctaaacat atttaaaaaa aaagtatata aaataaattt gatttatatt atatagattt
     8581 aaagtaaaaa tagttttat
//
LOCUS       DAEPOZ010000158         8531 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.158, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000158 DAEPOZ010000000
VERSION     DAEPOZ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8531
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.158"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(91..363)
                     /locus_tag="KR440_002669"
     CDS             complement(91..363)
                     /locus_tag="KR440_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699704.1"
                     /translation="MYDEIIEIYFDYWINEHMYRSILEKDEYYIECWKEVKKINELLE
                     INLEDDKKYLLVELNDLFNSLIEFERELSYKLGFKTGAKFIIELLK"
     gene            complement(613..1947)
                     /locus_tag="KR440_002670"
     CDS             complement(613..1947)
                     /locus_tag="KR440_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0699705.1"
                     /translation="MNNSNNLIKNTEAEQSILGSILLDSTIVEELEQMLNEDMFYHEE
                     HKYIFNAIKEINKRNEVIDIITLTEELKRKGHLNNIGGISYITSLSTIVPTVSNFTSY
                     VSIVIEKYKNRNIIDKFNKFNLGEIDDNTLIYQLENDINKANQKLIKKDSSITAISER
                     AFEYINDEMNEGIKTGIKFLDDTIGGLYGGELTTIAAKSGRGKTALALQILRNVIFQG
                     KKVLFISGEMSDIQILFRNIASLTGISVVKMKNRNLNDDEIVKYIQALSIINQENNLY
                     INDSVNTVSGIKREIKSTKPSLVIIDYLQILDYEGKEVGEAKFADLSRQIKKITLDFN
                     IPVIQLAQLNDELKDERPKGDRVMRSSKQIYMDSNSVIYIHRPTEKEALNYCKTMGIE
                     DIRELRRREKGFGANLSEIIVAKNRDGESGFRPYWYIGNLFSFEECKKIQEI"
     gene            complement(1960..2703)
                     /locus_tag="KR440_002671"
     CDS             complement(1960..2703)
                     /locus_tag="KR440_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699706.1"
                     /translation="MSNKGWIKLYRELLDKSIWKDIKPERKVILITLLLMASHKENHV
                     FIDNKRIKIKSGQFISSYSKIAERCGKGVSYQMVRSCLLVFKNAGFLTYETTNKYIVI
                     TIENWELYQENIENQQPNQQTINNQSTTIKNIKNENNIIYSEIISKFNSTCIELSKVK
                     KLTDTRKRRINSIIREYDKEAVLEVINIVSKTSFLNGNNNTGWKANFDWIMNSSNFTK
                     ILEGNYHSKEIVINKETGIYNPDDSQLKW"
     gene            complement(2725..3039)
                     /locus_tag="KR440_002672"
     CDS             complement(2725..3039)
                     /locus_tag="KR440_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699707.1"
                     /translation="MKENNNLEVLKTLQATLNIIVKNEEEAQEKKQGYKIIENAKRNK
                     AELITCKEASKLYPIGESKFRQLCHSKNKGFPCIWINNRVYIIKNKLDDWFIENANGI
                     KL"
     gene            complement(3043..3243)
                     /locus_tag="KR440_002673"
     CDS             complement(3043..3243)
                     /locus_tag="KR440_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0699708.1"
                     /translation="MGINLKIFRIKKGLKQQELADRVGISRYYLSNLETGKANNPSND
                     LMIKLSRALDTTVEELFFSKEE"
     gene            3390..4154
                     /locus_tag="KR440_002674"
     CDS             3390..4154
                     /locus_tag="KR440_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0699709.1"
                     /translation="MQIGKNLKKIRKQKELTQIQLAEISGISRNALINYENDKRIPSI
                     DTLSKLAKALKIEKTIFYFDIENEVMTNEDVLYFLQSLFPEEYYSFDEFLNAISNLME
                     DDDIRITSSLLLGAWSDKDQYYKLGEKLELNENQVYNWILSDCVSNTELDFSLHPDDI
                     KEIIKNDVLIKQSIDELLDIGLSEENIEAIYDYFSEATDNGSNIRLKKLISQKENMTI
                     KNNDYFKLLQENNELKEKNKKLENTLKEIKKLLTKI"
     gene            4405..4896
                     /locus_tag="KR440_002675"
     CDS             4405..4896
                     /locus_tag="KR440_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0699710.1"
                     /translation="MINNTIGEKIKQYRKSINMSRNELANKIGCSVHAIAKYEQNQRI
                     PSLDMIRKISTALSIAINKLIVEENDLDTLTNNNINLKVFDKELDLVVHILENYGYDI
                     KTDYKKKFEITDNADTIKPISLSFSDFKDFSKNLNWVINGLVENFIQENSNGLIEDED
                     EEY"
     gene            4988..5740
                     /locus_tag="KR440_002676"
     CDS             4988..5740
                     /locus_tag="KR440_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBH0699711.1"
                     /translation="MNKSYIPKYTHKFPTNLPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYIIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNNLFISDISIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILISNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDGYNYKSYKELFKNGN"
     gene            5730..6896
                     /locus_tag="KR440_002677"
     CDS             5730..6896
                     /locus_tag="KR440_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBH0699712.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPIRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKDLRVSHMQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENRLEILTLEQQKNFIKAIEGHKLEVLFLVALGTGLRLGELLGLKW
                     FDIDFKNSNLTVKRTLQRTYFIDKNGNRELKVLEQEPKTSNSYRTVPIPKDVLNKLKE
                     HKVNQNVDILKAGELYRNDNYVFCNELGVPIDDKRPLRNLKSILSSLDIEPIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKVEAVEKLDKIFSL"
     gene            complement(7012..7242)
                     /locus_tag="KR440_002678"
     CDS             complement(7012..7242)
                     /locus_tag="KR440_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699713.1"
                     /translation="MEANKTLLQILYKKIILEFSNQTGKSLEESMDYLYTSETYKLIS
                     EGVSDMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(7248..7727)
                     /locus_tag="KR440_002679"
     CDS             complement(7248..7727)
                     /locus_tag="KR440_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HBH0699714.1"
                     /translation="MIVYHDSYCVAKTPNIIFLRDSLDFGKGFYVTSIKTQAINWIER
                     FKKRGKKAYLNSYSLDIDKLKENCKVKIFNSYNLEWLDFILECRNMSDVYMKYDVIIG
                     GIADDKIYNTIELYQDNLIEKDEALKRLKYYKPNEQICITNQNVIDKYLKFRCEEEV"
     gene            complement(7727..7942)
                     /locus_tag="KR440_002680"
     CDS             complement(7727..7942)
                     /locus_tag="KR440_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HBH0699715.1"
                     /translation="MDERKLEFSIFCIEALAEKLNIDSEGVYKLLKHDTDILDNYIIP
                     CYEALHSQSKSYIVEDLIQVLEEKGVM"
BASE COUNT         3099 a         1247 c          942 g         3243 t
ORIGIN      
        1 ttaatatata cttcacttaa aatattctag tacaacttat taataatctc gatttactat
       61 ttaattattc tattgagata cacaaattaa ttacttcaac aactctatta taaattttgc
      121 tcctgtttta aatcctaatt tataactaag ttctctttca aattctatta atgaattaaa
      181 caaatcattt aattcaacca acagatattt cttatcatct tccaaattta tttccaataa
      241 ctcattaatt tttttaacct ctttccaaca ttcaatataa tactcatctt tttctaaaat
      301 acttctatac atatgttcat ttatccaata atcaaaatat atttctataa tttcatcata
      361 cattttagta cctccttgac tttcatcagg tacttaatat atacttaagt tgctaggtta
      421 gtgtacatta agtacctaat ttataaaggt agttgttata tttatatggc aactaccttt
      481 ttctaataag catttaaatc taaactcaat atattttaaa tctctattac atctaattaa
      541 gttttatata tttaccattc aatacttctt gaaacataat ttatctatta cttaataaat
      601 catgcttctt ttttatatct cttgtatctt cttacattct tcaaaagaaa acaaatttcc
      661 tatataccaa tatggtctaa atcctgactc accatctcta tttttggcaa caataatctc
      721 acttaaatta gcaccaaaac ctttttctct tcgtctcaac tctcttatat cctcaattcc
      781 cattgtcttg caataattaa gagcctcttt ttctgtaggt ctatgaatat atataactga
      841 attagaatcc atatatatct gctttgagct tctcattact ctatcccctt taggtctttc
      901 atccttcaat tcatcattta attgagcaag ctgaataact ggtatattaa aatctaaagt
      961 aatctttttt atttgccttg ataagtcagc aaatttagct tctcctactt ctttaccttc
     1021 ataatccaaa atttgcaaat aatcaattat gactaagcta ggcttagtac tctttatttc
     1081 tctctttatc cctgatactg tatttacaga gtcatttata taaaggttat tttcttgatt
     1141 tattatgctt aaagcttgta tatatttgac tatttcatca tcatttagat ttctattttt
     1201 catttttaca actgatattc cagttagtga ggctatattt ctaaacagaa tctgtatatc
     1261 actcatttct ccacttataa aaagtacttt ctttccttga aatattacat ttctaagtat
     1321 ttgtaaagct aatgctgttt tcccccttcc acttttggct gctattgtag ttagttcacc
     1381 tccatataaa cctcctatag tatcatctaa gaactttatt ccagtcttaa taccttcatt
     1441 catttcatca tttatatatt caaaggctct ctctgatatg gctgttatac ttgaatcctt
     1501 ttttattaat ttctgattgg ctttatttat atcattttct aactgatata taagagtatt
     1561 atcatctatt tctccaagat tgaacttatt gaatttatca attatatttc tatttttata
     1621 tttttcaata actatactga cataactcgt gaaatttgat actgttggga ctattgttga
     1681 caaactggta atatagctta tacctccaat attattcaaa tgaccttttc tctttaattc
     1741 ttctgtaaga gttattatat ctattacttc atttctctta tttatttctt ttatagcatt
     1801 aaatatgtat ttatgttcct catgataaaa catgtcttca tttaacattt gctctaactc
     1861 ttctactatt gttgagtcta ataaaatgct tcctaatatt gattgctctg cttctgtatt
     1921 tttaattaag ttgtttgaat tatttataat ataacctcct taccacttta attgtgaatc
     1981 atctggatta tatattcctg tctctttatt tatgacaatt tccttgctat gataattgcc
     2041 ctctagtatc ttggtaaaat tacttgaatt cattatccaa tcaaaatttg ctttccatcc
     2101 tgtattatta tttccattca gaaaacttgt tttactaact atatttataa cctctaatac
     2161 agcttcttta tcatattctc ttattattga atttattctc ctctttcttg tgtctgttag
     2221 tttcttgacc tttgaaagtt caatacaagt tgagttaaat ttgcttataa tttcactata
     2281 tataatatta ttttcattct ttatattctt gattgttgtt gattgattgt taatcgtctg
     2341 ttgattaggt tgttgatttt ctatgttctc ttggtaaagc tcccagtttt caatagttat
     2401 gactatatat ttgtttgttg tttcgtatgt tagaaaacca gcatttttaa aaaccaacaa
     2461 acaacttcta accatttgat aactaactcc tttaccacat ctttcagcta ttttgctata
     2521 agaagaaata aattgtccag actttatttt tatcctttta ttatctataa aaacatgatt
     2581 ttctttatga ctagccataa gtaatagagt tattaatatt acttttcttt ctggtttaat
     2641 atctttccaa attgatttat ccaacaactc cctgtaaagc tttatccacc ctttattgct
     2701 caattgcaaa cacctacttt aagactataa cttgatacca tttgcatttt caataaacca
     2761 atcatccaat ttgtttttta ttatgtatac tcgattattt atccatatac aagggaaacc
     2821 tttattttta gagtgacata actgcctaaa ttttgattct cctataggat aaagcttaga
     2881 tgcttcttta catgttataa gttcagcttt atttcttttc gcattttcaa taattttata
     2941 tccttgcttt ttttcttgtg cttcttcttc attctttaca ataatgttta aagtagcttg
     3001 taatgtcttt aaaacttcta aattattatt ctctttcata ttttactcct ccttactaaa
     3061 aaataattct tctactgttg tatctaaagc tctagatagt ttaatcatca aatcattgct
     3121 tggattatta gccttacctg tttccaagtt tgacaaataa tatcttgata taccaactct
     3181 atctgccagt tcttgttgct ttaaaccttt tttaatccta aatattttta aattgattcc
     3241 catacttttt cctccttttt tctagtttac attcgtaaac tttacatttt aattataatg
     3301 tcgacatatg tgtgctgtca ataagaatat attatttttc tgttctcatt tgtgtacttt
     3361 tgggatatac tttatacgag gtgataatat tgcaaattgg aaaaaactta aaaaagataa
     3421 gaaaacaaaa agaactaaca cagattcaac ttgctgaaat tagtggcatc tcaagaaatg
     3481 ctctaataaa ttatgaaaat gataaaagaa tacctagtat agatacctta tcaaaactcg
     3541 ctaaagcttt aaaaattgaa aagactatct tttattttga tattgaaaat gaagtaatga
     3601 ctaatgaaga tgttttatat tttttacagt ctttattccc agaagaatac tattcttttg
     3661 atgaattttt aaatgctatt tcaaatctta tggaagatga tgatattagg attacctcaa
     3721 gtttattgct aggtgcttgg tctgataaag accaatatta taaacttggt gaaaaattag
     3781 aactaaatga aaatcaagtt tataattgga tactttcaga ttgtgtatct aatactgaat
     3841 tagatttttc attacatcct gatgatataa aagaaattat taaaaatgat gttcttataa
     3901 aacaatcaat tgatgaatta ttagatattg gtctatcaga agaaaatata gaagcaatct
     3961 atgattactt tagtgaggca acagataatg ggagtaacat tcgactaaaa aagttaatat
     4021 ctcaaaaaga aaatatgact attaaaaata atgattactt taaattatta caagaaaaca
     4081 atgaattaaa ggaaaaaaat aaaaagcttg aaaatacact taaagaaata aaaaaactac
     4141 taactaaaat ataaaataat atcaatacta tctaaagcaa tgataaaaaa ctacaaatgg
     4201 agttacaatt attaataaag catatatgct aaaatacaca tataaatacc ctactaattt
     4261 attttatgga gataaaaaat atactctaac atacattaaa agaattatga gtaagttagt
     4321 atatgatata gataatctag aaaataactc ctttatatgt gtaaaaatac aatcattgtt
     4381 tgatgatata tttaaggtgg aattatgata aataatacaa tcggagaaaa gattaaacaa
     4441 tataggaaat cgataaatat gtcaaggaat gagctagcca acaagattgg ttgttcagtt
     4501 catgctatag ctaaatatga gcaaaatcaa agaataccta gtctagatat gataagaaaa
     4561 atatctactg ctttatcaat tgccataaat aaactcattg ttgaagaaaa tgatttagat
     4621 actttaacaa ataataatat taatttaaaa gtatttgata aagagttgga tttagtagtt
     4681 cacatattag aaaattatgg ttatgatata aaaacagact ataaaaaaaa gtttgaaatt
     4741 acagataatg cagatacaat aaaaccaatt agcttaagct tctctgactt caaagacttc
     4801 tccaaaaatc taaattgggt gataaatggt cttgttgaaa attttataca agaaaactct
     4861 aatggtctta ttgaagatga ggatgaagaa tattagtatt gcacattttc aaaatatggg
     4921 tttacctatt aacttttata cattcatagt taataaataa aaattagata tgaaaggagc
     4981 taaaatcatg aataaatcat atataccaaa atacacacat aaattcccta cgaatctacc
     5041 tcatggagat aaaaagtata ctttaacata cattagaaga atgataagtg aatttgtata
     5101 tgatatgggt aacctagaaa ataacccctt cacattccca gaagtacaaa cattacttga
     5161 tggtataaca gtaggtggac ataaattaag tgaccaaaat caaatactaa atataaaatc
     5221 atcatgggat tatataataa aattaagtaa caaagaaaac ttatctttaa ataaagatac
     5281 aatttgctct atacataaaa tagttgcaaa agatgaagct ctaattgttg gagattttag
     5341 aaacggtaac ataggtatag ctggtactac tcaatatgag tgcatagaag ccactctatt
     5401 aaataattta tttataagtg acataagtat cataaataaa ataactaacc ccttagaaaa
     5461 ggctataata ataaatttat ggttgtccta ttgtcaattc ttctatgatg gtaacaaaag
     5521 aactgctcga cttacctcaa acctaatact tataagtaat gatataggag ttctatcaat
     5581 accagccaaa cataaacaag aatacaacac attaatgcta aacttctatg aaacactaga
     5641 agctgatgaa gttataaaat tcttattaga gaaatgtatt acattcttcg atggatataa
     5701 ctataaatca tataaggagt tgtttaaaaa tggcaactaa aagagctaat ggagaaggct
     5761 ctatcggaaa atataaagat ggttggcgtt ctcgtataat gattggttat aatgaagatg
     5821 gaaaaccaat aagaaaagaa ttttatggta aaactcaaaa ggaagttaaa gataaacttg
     5881 atgcttataa aaaacaatat tatttaagtt ctgatatttc agatgataaa ataactttag
     5941 agcattggtt ttacaattgg ttgtttgaat atagaataaa agatttaaaa ccaaagtcat
     6001 ttgaaagata tgaaagcatc tatcgtaatt atattaaaga tactgattta ggcaatgtta
     6061 agttaaagga cttaagagta tctcacatgc aaaaatatta caatagattg cttgattcca
     6121 ataaaagtat acctactata aggcaaataa acactaaact gaaaacctgc ttatcagaag
     6181 ctgaaaaaca aggtcttata caacgtaatt attgtactat ggtaaatata ccagttgaga
     6241 aaaaggaaaa taggcttgaa atattgactc ttgaacaaca aaagaatttt ataaaagcta
     6301 tagaggggca taaattagaa gtattatttt tggttgctct tggtactgga cttagattag
     6361 gagaattatt aggacttaaa tggtttgata ttgattttaa aaattctaat ttaacagtta
     6421 aaagaaccct tcaaagaaca tattttatag ataaaaatgg aaatagagag cttaaagtat
     6481 tagagcaaga accaaagaca tcaaattctt acaggactgt acctattcca aaagatgttt
     6541 taaataaatt gaaagagcat aaagtaaatc aaaatgtgga tatattaaag gctggagaat
     6601 tatatagaaa tgataattat gtattttgca atgaattagg tgtccctata gatgataaaa
     6661 gacctttaag gaatttaaaa tctatattaa gcagcttaga tatagaacct ataaagtttc
     6721 atggtcttag aaaaacttat gcaactagac tttttgagaa tgatgttcct cctaaaactg
     6781 tacaagtttt aatgggtcat tatgatatat caataacttt aaatatatat actcaagtta
     6841 tggaagataa gaaggttgaa gctgtagaaa agttggataa aatattttca ttgtaaaaat
     6901 caaatacata aaaatactat gatttcatat agttaattac aaaacccaaa taaggataca
     6961 aattaatttt atattatctt gtatccttaa ttcgtatcta gttttaacta tttatcttgg
     7021 atagccttta tgatacatta atccattttc tagcattaat tcttgagcca agtatatatg
     7081 acccttacag tgcatatctg atacaccctc acttataagt ttatatgttt cagaagtata
     7141 taaataatcc atactttctt ccaaactctt gcctgtctga ttagaaaatt ctaatataat
     7201 ttttttatat agtatctgta ataatgtttt atttgcttcc ataagcacta tacctcctct
     7261 tcacatctaa actttaaata tttatctatt acattttgat tagttataca tatttgttca
     7321 tttggcttat agtattttaa tctttttaaa gcttcatctt tttcaataag attatcttga
     7381 tacagttcta ttgtattgta aatcttatca tcagcaattc cacctattat aacatcatat
     7441 ttcatatata catcactcat atttctacat tctaatataa agtcaagcca ttctaaatta
     7501 taactattga agatttttac cttgcagttt tcttttaatt tatcaatatc tagagaatag
     7561 ctatttaaat atgctttttt accccttttc ttaaaacgtt ctatccagtt aattgcctgt
     7621 gtttttatac tggttacata gaatcctttt ccaaaatcta aagaatctct taagaagata
     7681 atattaggcg tttttgccac acagtatgaa tcatgatata caattattac atcacgccct
     7741 tttcttcaag cacttgtatt aaatcttcca ctatataact tttactttga gaatgtagtg
     7801 cttcatagca aggtattata tagttatcta aaatatcagt atcatgttta agaagtttat
     7861 aaactccttc tgaatctata ttcaactttt ctgctaaagc ttcaatgcaa aaaatagaaa
     7921 attctagttt tctttcatcc attacaaacc tcaactccta tccacttata taatatatat
     7981 atttttatta attaataact taaaccatct tatgtttata aaaatattat atcataaagt
     8041 agatattaaa attctatgtt acataatatc ataaacaaat aaagactagc atttccaaat
     8101 atttcatcat ttatttttta actcagggaa gatataccac ttgaacaata tcctcaactg
     8161 atattttgat atattaaatt tctattataa aaattttatt ctaattataa atcatatagt
     8221 aatatacatt cattacttta taatttgaaa cctcttacac taataaaatt tgaaagtgct
     8281 cattattcaa tacaaacatg cctaaaatta tattttaatt tttacactat caaaaacata
     8341 tatattaaat ttgacccact taaatacagt aaattaattt ctacaacatg atataattaa
     8401 attatgacaa aagactttga tatttataaa aactttccaa cagatgtctc ttataagatt
     8461 tttttatata atcttaaatt aaaaaactca ttaatatgcc cctattgtta ttctgataaa
     8521 attagtactt a
//
LOCUS       DAEPOZ010000159         8482 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.159, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000159 DAEPOZ010000000
VERSION     DAEPOZ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8482
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.159"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     misc_binding    98..309
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            472..1761
                     /locus_tag="KR440_002681"
     CDS             472..1761
                     /locus_tag="KR440_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBH0699716.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     gene            1815..2894
                     /locus_tag="KR440_002682"
     CDS             1815..2894
                     /locus_tag="KR440_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HBH0699717.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELCGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            2915..4459
                     /locus_tag="KR440_002683"
     CDS             2915..4459
                     /locus_tag="KR440_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBH0699718.1"
                     /translation="MYRVGIDVGGTNTDACILDGELKVIHSVKVATTKDVETGVYNAL
                     KKVIDESKIDHSLIKYAMLGTTHCTNAIVERKRLNNVAMIRIGKPATTAILPFIDWPE
                     DLREKIELDSILVSGGYEFDGRKINELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQ
                     QEEEVAKWVREILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALK
                     DLGIDAQIYFSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGG
                     TTSDLGVLYNGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPES
                     VGYRITEDAIIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLED
                     AVDQMKTSQDDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVE
                     KVYSLDKMSREDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVK
                     VVGDLI"
     gene            complement(4622..5164)
                     /locus_tag="KR440_002684"
     CDS             complement(4622..5164)
                     /locus_tag="KR440_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HBH0699719.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDNKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(5312..5596)
                     /locus_tag="KR440_002685"
     CDS             complement(5312..5596)
                     /locus_tag="KR440_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBH0699720.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(5659..6423)
                     /locus_tag="KR440_002686"
     CDS             complement(5659..6423)
                     /locus_tag="KR440_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBH0699721.1"
                     /translation="MNNYLKFATYQLIGFTSSIPSLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
     gene            complement(6705..6971)
                     /locus_tag="KR440_002687"
     CDS             complement(6705..6971)
                     /locus_tag="KR440_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBH0699722.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTDIEKPFWGEQCPVKSCCED
                     KNLIHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
     gene            complement(7014..7823)
                     /locus_tag="KR440_002688"
     CDS             complement(7014..7823)
                     /locus_tag="KR440_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0699723.1"
                     /translation="MNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKEKL
                     TLIPDASGCMIFKFDKKGIESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTG
                     LSQRESTDLKISLEDFNTLFSLEVNSIFERTSTIKELVEQLDMLFLSYLLKSNIVDMT
                     IPILENARKQNSIMSVKNISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEV
                     QNNKRPFATLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
     gene            8136..8417
                     /locus_tag="KR440_002689"
     CDS             8136..8417
                     /locus_tag="KR440_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699724.1"
                     /translation="MASKQTPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
BASE COUNT         3017 a         1039 c         1370 g         3056 t
ORIGIN      
        1 tataaaacta aaaaatgtaa gtaattgact ttttaagttg acaaaatatt gcatatatat
       61 tatgatataa atcataaatt aactacaaat caatattata tattcgtaga agaagaaagt
      121 agcataaagg aattttacag agagttagat ttggctgaga actaatattg gaatttatgt
      181 gaaaaggact ttggagaata ctattgtaat tatgttagaa tagtggttta attcgcctta
      241 agaaaatgag tgagtaaaat taatttttac taataagagt ggtaacacgg atttatattc
      301 gtctcttgga tttttccaag aggcgttttt tattataaac ttatttcaga aaatttaaaa
      361 ctatatatta aatgaaaatt ataaaaatat gatagttaat aaatttataa attagaaaat
      421 tgtaaaatat gctaaggtca gtagttttac gatttaaatg gagggatgaa aatggaagaa
      481 gaaaaaatta actatgctct ggaacagatt ccagagaatc aaaaacgtgg ttgggtagct
      541 atgttttcag ttttagtagc aataggtgtg gatttatcat ctgttatttt aggcgctgag
      601 ttggcacaga gtatgccaat gaaacaagca atattgtcag taattgttgg ttcatttttt
      661 tctgcaattt tgtatactac atgctctttg gttggttcat ctacaagttt atctacatct
      721 atgataacaa agtatgtatt tggagaagct ggagcaaaaa tattttctct agttataggg
      781 gtttctctat taggttggtt tggagttcaa gtaggtttct ttgcacaaaa tgcacagata
      841 ataataaaag atatatttaa tttagatgtc agtatgcaga ttttaagcct aataggtggt
      901 ctcttgatga tgagtactgc aatttatgga tataaggcca tggagaagtt gagtgtgtat
      961 tcagtaccat ttttattagt tcttatgatg ctaactatat tcttagcttt tagagcaaat
     1021 ggaatttcag ttgatgataa tatgaaatct actatgacat ttgcaggtgg agtttctctg
     1081 tcaatgagta taataatagt tggtgcaatt gtatctcctg atataagtcg ttgggctaaa
     1141 agtagaagag actgtgctct ttcaagcttt ttgggaatac aatttggaaa tgcttttatg
     1201 ataatagttt cgatagtatt agtaaaatgt atgggaactt cagatattat gagaattttt
     1261 attacattag gaattgctat accaggtata atagtactta cattagcaca atggactaca
     1321 aatacaagta atgtatattc agcatcatta agtatagctc ttgtacttaa gaaggctcct
     1381 gagaaagtat taactatagt ccttggtatt atagcaacat tattggctgt atttggaata
     1441 tatgaaggtt ttataggatt tttaaattta cttggaattg taattgcacc tgttggtggt
     1501 gtatatacag cagaatatta catagtaaag caagaactta aaggctttga taaaggagta
     1561 ttatataaac caatagttaa acgttcaata gtgtcttgga taattggtat attaataact
     1621 tacctttcaa catatggatt tataactctt actactatag cacctttaga tggttttata
     1681 gcagggtttg tagtacaatc tattattgga aaagtgcttt gttcaacaaa aaataagcaa
     1741 gaaatagaca aagcagtata aagaaatttg tttataaagt ataaaataaa ttagaaatat
     1801 gaggagaaag taacatgaga tttttaacag aagaaagtgt agataaaata gcagttggtg
     1861 cagcagtgct tggaactgga ggtggaggtg acccatatgt aggtaaattg gttgcaaaac
     1921 aagccattaa aaagtatggt cctgtaaaag taataagttt agatgaactt gatgatgatg
     1981 ctttggtagt tccagtatct ggtatgggtt ctcctgtaat tacaatagaa aaattacttt
     2041 cagaagtaga gttaactacc cctttagaaa taatggagaa attattaaat agaaaagtgg
     2101 atgttataat tcctatagaa ataggaggga taaactcact tatgcctata gcagttgctg
     2161 ctaaaaaagg acttcctatc ttagatgcag attctatggg tagagctttc cctgaagctc
     2221 aaatggtaac tttttatctt gaaggatatg aggcatctcc tgccgtaatg tctgatgaaa
     2281 aaggtaattc tgccatcctt tatcctgtgg atggaatttg gtcagaaaga ttagctagaa
     2341 ctttaactgt agaaatggga ggaagttctt ctattagtga ctataattta agtggagctc
     2401 aagttaaaaa agctgctata ggtaatactc ttacaatagc tgaaactata ggagggtttt
     2461 tacttgaaaa taaatctgat tcaaaaggtg ctgttgagaa tatactaaaa gaattgtgtg
     2521 gatacaagtt atttgaagga aaagtaattg atattaagag agagttgaaa ggtggattta
     2581 ctagaggaca tgcctttttt gctgggatta atgagtatga tggagagtat tctatattat
     2641 tccaaaatga gaatttaatt gccaaaaaag gtgatacacc actttgtatt acaccagatt
     2701 taatagcagt attagattta gaaactggat ttccaataac tacagagaga ataaaatatg
     2761 gctctcgtgt tatggtagta gcttttcctt gtaatgaaaa gtggagaaca gaaaaaggaa
     2821 ttgagacagt tggacctgga tactttggat atgatgtgga atataaaaca gtagaagaat
     2881 tacaaggaaa ataaaagttt aggaggaaat tttgatgtat cgtgtaggaa ttgacgttgg
     2941 aggaacaaat acagatgctt gtatactgga tggggaatta aaagtaattc attcagttaa
     3001 ggtggcaact acaaaagatg ttgaaactgg tgtttacaat gccttaaaaa aagttataga
     3061 tgaaagtaag attgaccatt cattaataaa atatgctatg cttggtacaa ctcattgtac
     3121 aaatgctatt gtagaaagaa aaagacttaa taatgtagct atgataagaa taggaaagcc
     3181 ggccactact gccattcttc catttataga ttggccagaa gacttaagag aaaaaataga
     3241 attagattca atattggtat ctggtggata tgagtttgat ggtagaaaga taaatgaact
     3301 ttgtaaagat gaagttgtag agttttgcaa tagaataaaa ggcaaagtgg aaagtgttgc
     3361 aataagtgga gtatttgctc cagtaaataa acaacaagaa gaagaagttg caaaatgggt
     3421 tagagaaatt ttaggggata ttcctatatc tttatcaaat gaaataggta atataggtat
     3481 attagaaaga gaaaatggag ctattttaaa ttctgcattg tctcaagtag gtaaagctgt
     3541 atctttagga tttgaaaaag cacttaaaga cttaggtata gatgctcaaa tatatttcag
     3601 tcaaaatgat ggaacattaa tgaatttaga gtatacaatg aaatacccaa tttttacaat
     3661 tggatgtgga ataacaaatt ctataagagg agcttccttc ttatcaaaag taaaaaatgc
     3721 aatagtatta gatgttggtg gaactacaag cgacttgggt gtactatata atggattccc
     3781 tagagaatca tctattccag ttacaattgg aggagtacat acaaacttta gaatgccaga
     3841 tgttttaact attggtcttg ctggtggtac atgtgtaaaa ggcgaaaaag ataatataaa
     3901 gataggtcct gaaagtgtgg gatatagaat aactgaagat gcaataatat ttggtggaga
     3961 cactctaact ttatcagatg ttgttacaaa attagatatg gcgtttgaag atagaaaaag
     4021 caatgttgaa aacttagata aagagtattg taatgaggta tataagaagg ttattttaaa
     4081 attagaagat gctgtagacc aaatgaagac tagtcaagat gatgttgaat tggttttaac
     4141 tggtggagga agtataattg ttcctgaaaa actaaaaggc ataagcaatg ttataagacc
     4201 tcctcattat actgctgcaa atgctattgg tgcagcttta ggacaaataa gtggagatgt
     4261 agaaaaagta tacagtttag ataaaatgtc gagagaagat gccatagaag atgcaaaaaa
     4321 agaggctgta aataaagctc taaaagctgg tgctaaagaa gatactatag aagtattaaa
     4381 tatagaagat gttccacttg cataccttcc tggtaatgca cttcttataa aagtaaaagt
     4441 tgttggtgat ttaatttaaa gaataaatat aagtatttcc agaattatat aaatatttct
     4501 ggactcattt ttaaacttat tttatcaata aaaacagcat aacttttgac tatattctta
     4561 taagtttccg aacagccgat atatacctat tttttgaggt ttatcggctg tttgttatct
     4621 tttatcgcat tccaactatt tttcttaccg tatttgctgt acgaattgta atcttatcac
     4681 ttacatttga actggcagtc tttgaccacc aatttgtctt tttataatct ttacgactaa
     4741 aagaccaaaa gatagcattt ttataatgat atactttttc atactcttct ttaggatttc
     4801 caacctcatc ataaaactcg tcataagata agtcaggaaa cataaaaata agattatcat
     4861 atatttcttt gttgttatct ccccaccaat taggagcatt atttagtata tcttttaact
     4921 cctcttgtga aataataaaa actggtattt ccaattcaaa ctgttttttt atcattaatc
     4981 ttattgtatt tgagataacc ttgttgtcat ctacatcact taaaaagaca acattaccac
     5041 tattaaggta tgtagaaact tctgaataac caagttctac aaagcttgct tttaattcag
     5101 acataattat cttgttctta ccacttatat tgatacctct taataatgcg atataacttt
     5161 tcatataata agttcacctc taaacattca catttttcta tgtagtacaa actggaattt
     5221 gcatctttta taattctttc aaaacttctt catatgtaat ttttttaata taaaaaaatc
     5281 ggtgcttaat ttgtaccgat ttttattttt cttagtaaag aactcgtttt tttgcttctg
     5341 ctatagctct agcagcactt tttgcttgca ttcttttttc ttcttctttt ttaaattcat
     5401 ttattgtttc atttagattc ttatcatgtt catttttttc tttatcttcg ttttctctgt
     5461 atactaatat ataatctatt attgattcgt ttattatctg agatatactt ttattttcat
     5521 taattcctat tctcttaact tccttaagta gagtctcatc aatatgaacc gttgtttgct
     5581 ttgtattttt tgccacctta atcacctctt aaaaaaaatt ctaacattaa aatttttttt
     5641 agtcaataaa ttttaatatt aaaattcttt aaatgtttta tttgattttc taaaattttc
     5701 tattgtattt atcatcttat tttttatatt ttcacctagt acagaagact ttagtctagg
     5761 attttctata tggtcataaa ttggttctaa aaattcgact tcgacaggaa cagagttaat
     5821 tttaccaata ggttggtatc cttcataagt atcttttgaa ttttttatta cgagtggaac
     5881 aatagggcat tttgctttat atgctatctt taaagctcca cttctaaact ccgaaaccaa
     5941 tgaatttggt tctttaatcc atgttaaatc tccttctgga aacactgcca tactttgacc
     6001 tgtaagtata ttttgagagg cttgggctat actctttata ccctcacgat tgttttttct
     6061 gtttacatat acacatctta gtaattttgc ccattcttta aatataggta tatttctcca
     6121 aactggttca tctgcaatta cacatccaat tggtctttct acacttgctg ttagtatgaa
     6181 gctgtcaagc atactagaat gatttacaac aaatagaagt ggttcttttg gcagtgtttc
     6241 cttaccaatt atattaagct ctatgttcac tatatctaaa gattttttag cttgtttttg
     6301 cataaattca aatttttcct ttaagctaaa tttatcagga ttctttttaa ttttaataag
     6361 gctaggtata gaagatgtaa aacctataag ttgatatgta gcaaatttta agtaattatt
     6421 cataaaatta tctccttaaa ataaaattat ttattactgt aaagatttta acataaaata
     6481 aaatttaagt ctattttaat tcaattttgt aacatatatt ttaaatagac agagattgag
     6541 attttatatt tctaatttta gtaatcagat tttatcaata aattactatt ctattaattt
     6601 aattaaataa cctttaaatt taattataaa aactgttgca gaaatttcta caacagttta
     6661 aaaaatttac cttacaaaaa cttattttat aacaagagag tatattataa ggctagtaca
     6721 gcccattttt tgcactgaga aattcttttg ccatcatcac cttgttcttt gtcatatgca
     6781 aattgattaa gcatttcaca tggaaatgta tcacatagac cacaatgtat aaggttttta
     6841 tcttcacaac aagattttac aggacattgt tcaccccaaa atggtttctc aatatctgta
     6901 caacctttac aattgacttt ttccttgtat ccacattcat aacataaaat tccacatctt
     6961 gattctatca ttttaagtca ctccttaaat ataaatttta ttatgattgt atatcaaaat
     7021 ttatactttt cattgtaaaa atcggacaaa ttttttatat aggttgttgg attaacacca
     7081 caaatagatt taaaatcatt tataaaatga gattggtcgt aataaccaat atcttgtgct
     7141 aaagttgcaa atggtctttt attgttttgt acttcttgta atacaaggtt tatacgaagt
     7201 aatctaaggt atgatttaac actcatccca agagaattat taaaaattct atttaaatgt
     7261 cgttcactat aacaactaat ttgtgaaatg tttttaacag acataataga gttctgtttt
     7321 ctagcatttt caagaattgg tatagtcata tctactatat ttgatttcaa taagtatgat
     7381 aagaataaca tatctaattg ttctactaat tcctttatag tacttgttct ttcaaaaatg
     7441 gaattaacct ctaagctaaa taatgtgtta aaatcttcaa gtgaaatttt taaatctgta
     7501 gattctcttt gagaaagacc tgtcaaataa tatactccac ctgggcgaaa ctcaacgaat
     7561 actctaaaaa gtacattttc tatatcattt tttacaatag tagtttttgt tgtggctccc
     7621 cagaatgcgc tttctatacc ttttttgtca aatttaaata tcatacatcc actagcatca
     7681 ggtattaatg taagcttttc tttaatactt gtatctggaa tagatattgt atagtgtgct
     7741 atatactgtc ttaacaattt atgtggcaaa atatatgtat actgtttgca ggtatttaaa
     7801 atgttattga ttattttatt cataagtata tcctcctgat atataaaaaa taagtatact
     7861 aatttatttt ttatataata aaccagtttt gttttaattg aaatattcta ataagatatt
     7921 ttgttaaatt tagatacaat aattgtaaag taaaatctca aaatatacaa ttattataaa
     7981 tcatagatat ttattaaata aatatattaa agaagaagga atataaaaca agattagaaa
     8041 ataatagtgt tactttcata ataaatacat ataaagatat ttttaagaat aaaatttatt
     8101 attaaataaa aaataaagga gagtgattta atcgtatggc atcaaaacaa acaccaagaa
     8161 acaatttaga aaagttttta agaataagag tcttatcaca tacaggcgat aaaatacata
     8221 taaagttacc tgtaaatttt gcaaaaagaa tgattgagaa taatgctttg gatttattta
     8281 atggaaaaga tgatgtagta gatggtaaaa aggttactga aatattagtc aaagcatttg
     8341 actatgattt aactggagaa gtagttcact tggagagaaa aaatggagat attattaaag
     8401 tttcaattgt acaatagaat tattttatat gaattttatt tttgaatagt tataaataat
     8461 tagaaatttg taaaaaagta ta
//
LOCUS       DAEPOZ010000160         8474 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.160, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000160 DAEPOZ010000000
VERSION     DAEPOZ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8474
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.160"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(141..1169)
                     /locus_tag="KR440_002690"
     CDS             complement(141..1169)
                     /locus_tag="KR440_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBH0699725.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDINKKMDTIDKLKDI
                     EEGYFYLLEDVYLDMKDVLEDLMLYRMNEDEYKYEIKNIIKMFDEDLDNEKIYINKCN
                     NLNVVNKI"
     gene            complement(1217..2077)
                     /locus_tag="KR440_002691"
     CDS             complement(1217..2077)
                     /locus_tag="KR440_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBH0699726.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKSI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            2356..2565
                     /locus_tag="KR440_002692"
     CDS             2356..2565
                     /locus_tag="KR440_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBH0699727.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            2565..2831
                     /locus_tag="KR440_002693"
     CDS             2565..2831
                     /locus_tag="KR440_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBH0699728.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            2860..3432
                     /locus_tag="KR440_002694"
     CDS             2860..3432
                     /locus_tag="KR440_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBH0699729.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(3666..4856)
                     /locus_tag="KR440_002695"
     CDS             complement(3666..4856)
                     /locus_tag="KR440_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBH0699730.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENVENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKVSYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSAGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(5116..5547)
                     /gene="dut"
                     /locus_tag="KR440_002696"
     CDS             complement(5116..5547)
                     /gene="dut"
                     /locus_tag="KR440_002696"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBH0699731.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            5660..7888
                     /locus_tag="KR440_002697"
     CDS             5660..7888
                     /locus_tag="KR440_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HBH0699732.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEYEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(7999..>8474)
                     /locus_tag="KR440_002698"
     CDS             complement(7999..>8474)
                     /locus_tag="KR440_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0699733.1"
                     /translation="NILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAYFEEC
                     PMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDPKDRR
                     ILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKLG"
BASE COUNT         2958 a         1325 c         1057 g         3134 t
ORIGIN      
        1 acaataaaaa aatgtagaaa gtaagtatct ctctacattt tttattatat attattttta
       61 tattaattta attttatctt gattcagttt tataaaaaat tgttataatt gtcaagttct
      121 actctattat ttgtaatttc ttatatcttg ttaacaacat ttaagttatt acatttattt
      181 atatatattt tttcattatc taagtcttca tcaaacattt ttattatatt ttttatctca
      241 tacttgtact catcttcatt cattctataa agcattaaat cttctaatac atctttcatg
      301 tcgaggtaca catcttctaa taaataaaaa tagccttcct ctatatcttt caatttatct
      361 atagtatcca tttttttatt tatatctttc ataatttcta aattttgtat atttacatct
      421 atgattgtta ataagtacat tctcacagtt tttattattt caacagaaaa atctacaata
      481 ttataatttc cttctttttt atataatgac tccttaaatt tgttaaaaca attatctaaa
      541 ttagaattta tatcgttaaa tggtggtctt attatgtcac gtattattaa aagattttgt
      601 atatctacat tatttgtgat tgcctcatcc aaaataaata aattatatgc atcatttaat
      661 gggtcatggg catctccact aaactctaag ttaacaaatt caagtaaatt tttcaatgat
      721 atatagttca tacatctcat accacaattt atatattttt cttttatatc tacaaataaa
      781 tctctaatat tatttacaaa ccttggatgg ttattttttt tagcacttct catatctgtg
      841 ttattgaaac atgtcaaatc caaattacca aaagtatata ttcctttaat atcagaaaat
      901 atgccaagcc attttttaaa ttttctcatg acctcttcat aacttggtgc actcttcaat
      961 tcttcagaag atatatgtgt taattcttgt atatgaggat ataactcctc attacttact
     1021 ggtcttatta aagatttaaa tttatcaata ttttttgttt tttcatcata cataactgct
     1081 ccgattgcaa ttatatcaga attaaatact gctctttttg atttagaatt tggcatattc
     1141 atttcaaaat ctatatatac tcttttcaat ataaacaccc tttccaattg tttatagtga
     1201 ttctttttaa ctatttttat tcaaaatttt ctaagaaaga atgtttctcc ccatcttttt
     1261 gccaaccaag tactgaatca agatttacat tttctgctgc cttaaatata gatttttcta
     1321 catttttaaa atttgattct aagctcttta ttaagtcttt tatctcatat cttttattac
     1381 ctgttttttt agcagcaacc atacatgcac aatttaatgg ccatatacca ctattttgaa
     1441 taaatcttat aatatgttct tctcttatat aataaagtgg tcttataatt tttatacctt
     1501 caaaattagt agaattaagt ttaggaagca ttgttttaaa atttccagca cacaataagt
     1561 taagcattgt tgtctctatt acatcatcat aatgatgacc taatgcaagt ttgttacacc
     1621 ctaattcttc tgcctttgaa tataatgctc ctctcctcat tcttgcacac atatagcatg
     1681 gataatcttt tgctatttca tctgctatct caaatattct tgaatcaaat aaatgtattg
     1741 gtatatttag atactcacaa ttatctataa gtaattgtct tatatttgca tggtatccag
     1801 ggtccatagc aataaattca acatcaaaat taacttgtcc atgttttttt aactcttgaa
     1861 acattttagc cataagaata ctgtcttttc caccagaaat cgctacagct atcttatccc
     1921 cttcttcaac caatttataa tctcttattg acttcataaa cttagaccat aaattttttc
     1981 tatatttctt tattatactt ttttctatat ctcttactgg aagtctttcc tcaaaaggaa
     2041 ctataatttc acagcctttt cctgatagtt cactcatcta atcacctcgt atatttatgt
     2101 atatttatgt taataatatt ttacaaaaaa gtctaccttc atccacaaaa tactataatt
     2161 tttactttaa ttttactaaa taatttatag tttacaataa cacataaact tataataaca
     2221 taaataacct ctttctttta ttatcttttt tatttttctc atttaatatt ttattattag
     2281 taacgatttt taaatgattg aatattatac actagataga gttacctatc tatttacaac
     2341 aaggaggttt tcatcttgga aaataagact agagatatca aaaaaaattg tggtccatct
     2401 ttggctagac catacattaa taatcaaatt ttcacagaaa tgtactgcct ttctgatgca
     2461 ttaaaatttg gaactatatt tccagaacta aacttactag agtctgaaat gtacaacaaa
     2521 gaattatatg ctaagccaaa aaaatataga ggaggtagaa gataatgaaa gactcatcta
     2581 gagctgaact tatgagaaaa gttcaggaaa catcttttgc atgtgttgat atgaacttat
     2641 atttagacaa tcaccctgat gataaaaatg ctatcaacac ttataattca ttatgtaatc
     2701 aatttgcgca agctagatat gcttacgaaa ataaatacgg tcctttaact aactttgggt
     2761 atgctccaag tagatgccct tggcaatggg ctgaccaacc ttggccttgg gacagagaat
     2821 ttaattatta gacatcaagt attggaggat tgaaaattca tgtggatata tcagaaaact
     2881 atacaacacc cagttaatat aaaaacttgt gaccctagaa tggctaaatt tcttataact
     2941 caatttggtg ggccaaatgg agaacttgct gcatctttaa gatatttaag ccaaagatat
     3001 acaatgccta ctggtaatat gcgtgcactt ttaacagata ttggtacaga agagctagct
     3061 cacgttgagc ttatatgtac tatggtttat caattaactt ctgatgcaag cccagaagag
     3121 ttaaaagctg caggtcttgg ttcaaactat gctcaaaatg gatatggaat ttatccaaca
     3181 gattcaaatg gtgttccatt tgatgtaaga cctatagcag ttatgtcaaa tcccgtaacc
     3241 gacttacatg aggatatggc agctgaacaa aaagcacttg caacttatta tcaacttata
     3301 aacctaacag atgacgttga tgttatagat gtattaaaat tcttaggtca aagagaaata
     3361 attcactatc aaagatttgg tgaagcttta atggatgctt acgagttaga agaatctcaa
     3421 aaaatgttct aaaattttta tttgtataac tattattatt tgataattta taatttactg
     3481 ttgtgttaaa atttataaaa aatataaata taatttattt tctataaaca gagaaaataa
     3541 ataaaaagca ggtatcaatc aaatagtaac atgtattata ctatacatta ctatcaatat
     3601 tgtacctgct ttcatattat aagcaataaa gaaaaatcta tttattaggt tttataataa
     3661 aacaattata aaactcttct tgcatttaca tatctacttg aatagtagct actatttata
     3721 ttagatattg ttactttttt tgatgaacct gaagatgcat gtatcatatc tccatttcct
     3781 atgtatattc caacatgact tacagaacca ttatttgaac cttgtgtatc aaagaatact
     3841 aaatctccac ttctaaggtc tcctctattt acatatgttc catacttact ttggtctcta
     3901 gatactcttg gtatactaac tcctgcagat tttttcataa cgtactgtgt gaatccagaa
     3961 cagtcaaaac tatttggacc ttctgctccc catacatatg gttttccaag tagtgtcttt
     4021 gcaaaactta ttacactatc agctccttga cttggagaat tagtcgaata agatggaatt
     4081 gaagtatcaa cagaaccttt ttcagcaagg tattgacttg acacatatcc agtttgattg
     4141 ccataactta cttttgtcca tcctccactt tcagaaatac ttccaacctc agtaccataa
     4201 cttaatgtag ctattttaga atgacttgta cctggtcctg ttctaacgtt caatgacttt
     4261 gctgttacaa cctttgtacc ctttacaagg tcatttgaac tgttttcaac attttcattt
     4321 gtagagtcat ttacactagt tcctaaatat ttacttgaaa cataacctac tctaccatta
     4381 gaacttattt ttgaccatcc atttgactca gatatgtatc cgacttcagt cccatatcct
     4441 agtgttgata ttttagaact acttgtactt ggtcctgttc taaaatttaa tccttttgct
     4501 gttacaactt ttgtcgactt aactgaagta tcactacttt cattgctact tccaaggcta
     4561 tttattgcta cataatttcc atgcacataa cctgtgtttc cattatattt aacttttacc
     4621 caagaacctt cttttcctac gtactcaact ttatcacctt tgtaaagttt ccccattgat
     4681 tcatggtttg tacctggtcc ttttctaaaa tttaccgcat tccctgttat tgttctgctt
     4741 tctatactca cactctctac ttcagactca ttagcatatg ctaaattccc catagtaggc
     4801 attattatcg cagttgccat tatactggct gctattctct tttgattaaa attcataact
     4861 ttccactcat ctcctctttt ctactatctt tatttcactt caaaatataa aagaaataat
     4921 tgacactatt aaaatatcaa ttatttcttt tatattaata ttattacaaa tttgtaacta
     4981 tttgtctata gtaatatttg cttaccataa attaccatgt ataaatttta atcttcaata
     5041 gcctgtaatt tagtaatata gccatctttt acctttttta aaaatggcta tattttttaa
     5101 cttttttgta actttttaaa atccagttga gccaaaccca ccttttcctc tttcggagtc
     5161 acttaattct tttacttctt ctatgttaat atcatagata ggttttacta tcatttgagc
     5221 aattttcata tgcttttcaa ccttaaaatc atttttccca tgatttatta atataatttt
     5281 aagctctcct ctgtatcctt cgtcaatagt tcctggtgta tttaatacag taacagaatg
     5341 ctttaaggca agcccacttc taggtcttac ctgcgcttct gtcatagtag gaagctctat
     5401 acatatccca gttttgataa gtttagtctc tccaggaggt attacaacct cttctataga
     5461 atacaaatcc ataccagcat cacctttgtg agcaaaatta ggtattatag catcatcatt
     5521 taattttttt acttttaaat tgtacatctt atatctcctt tcaactttct ataaatatta
     5581 taacttaaat ttcgacctct tgaagttttt tatttttttg tggtaatata attattagtg
     5641 ttttaaagga ggatttttta tgaaacttgt acttgctgag aaaccatctg tggcaaaaac
     5701 tatagcatca tttttaggtg caaagaccag acaagatggt tactttgaag gaaatgatta
     5761 tataattact tatgcagttg gacacttagt atcattatat gatatgaagg attatgataa
     5821 agataaatac tctggctctt ggaaaatgaa caatttccca tttgttcctg aggataagtt
     5881 taaatttaag attgatagtt ctaaaactaa acaattcaac actgtaaaaa agcttttgaa
     5941 tagaaaagat gtagaatatg taataaatgc cactgacaat gaccgagaag gagaacttat
     6001 agcattttta atatttttac tggcaaaaaa taaaaaacca gtaaaaagaa tcttggtaaa
     6061 tgaatggaca ccagaagata ttacaagagg tataaaaaac ctaaaagatg aatatgaaat
     6121 gagaaatctt caagctgctg gatatactag gcttattact gactggttga ttggaataaa
     6181 tttcacttct gttgctacat tgaaatatgg aaatgggaaa ctattaaaca taggtagggt
     6241 aattcttcca acagttaaat tagtctatga tagagatatg gaaatactaa actttgttcc
     6301 taaaacttat tatgaaatag aaggacattt taaagctgaa gctggtgaat ataaaggtaa
     6361 atatgttaag ggtaaagaaa gtaaatttga tacacttgaa gatgcaaata aaattatagc
     6421 ttcaattaca tctgaaactg gtaaaatact agataaaaaa gttactatgt caaaggaata
     6481 tgcaccaaaa ctttttagtt tgacatcact tcaaggatat ataacttcta agtattccaa
     6541 ttttacatct gataaggttt taaatgtctg tcaatctctc tatgagggta atggtaaggg
     6601 tggatacata acttacccta gaactgattc cattttctta gaagaaagtt tggcttcaaa
     6661 agcttctcaa actttagata aattaaaggt tggtcttgaa tatgaaaata aaattaaatt
     6721 ttctaaaact aaaagagttt ttgattcttc taaagtagac tcccatagtg ctataattcc
     6781 aacttatata attccaaaaa gtttatctaa ggatgaacaa ttagtttatg atgccataaa
     6841 agatagattt gtagcaaact ttatgcctcc tgctgaatat gagaatacag aaattaagac
     6901 tgaagtagat aattgtactt tcttaacaaa aggtaaagtg cttaaatcaa aaggatacct
     6961 tgaggtatat aataaggaag aaaaaaacga tttacttcca cttgtaaata aagatgatgt
     7021 tgtagatgtg ttagaaataa aaccattaac taagcaaact actccaccta aaccatatac
     7081 tgaggacacc cttttaaagg ctatgaaaaa ttgtggtaaa aatgtgcctg aagaagatac
     7141 aacagtgcta tctggatatt cgattggaac atcagccaca cgtgctgatg tgttaaagaa
     7201 gataagtcaa gtgggctatg taaagaaaaa agggaaatct tattctataa cagaccttgg
     7261 gaaaaattta gttgaaattt ttcctgttaa agacttattt gatgtagatt atactggaaa
     7321 acttgaaaaa agtttaagcg atattcaaaa aggaaagtat acaagaaaag aatatcttac
     7381 taatatcatt agttttatat atcaaaatgt caatattata aaacaagatg cttcaaaaaa
     7441 tataaacaca gaagcatata ctttcgattc aaaaactaaa aaatttacta aaacaaaaga
     7501 aaaaaaacag aaaaaagatg taactaatac tgaaaaagaa gttgctatat caaaagattc
     7561 taataacaaa aaagaagtta aaaatggtga tgaaaatcaa tctcttggca aatgccccat
     7621 ttgtcaaggt gatgtactag aatttgacaa aggctttgct tgtaaaaatc ataaagaatg
     7681 taaatttgtt atttggaaaa atgataagta tttaagtctt tataaaaaga aagttaacaa
     7741 aacaatggtc aaaaatattt taaaaaaagg tgaaactaaa gtaaaatctt taactgctaa
     7801 aaacggtaat aaatttgatg ctacattaaa atatgataaa aatccagata ccggctattt
     7861 caattggaaa atagagtttg acaactaaat attcataaga aaagaaatag cagtagtata
     7921 ataatctata ttatattact gctatttctt ttcttatgaa gttttaactt ttttaatagt
     7981 gacttttaca atcacactct aacctaattt ctttactatt tctgataact ttaatgtatg
     8041 ttcatctaat aaagttagtt cctcttcaga aagattagat aattttttta ctagagattc
     8101 tttgagaata gcttttactg catcaaataa tttaaatgct ttatctgtta attcaaccct
     8161 aagaatcctt ctatcttttg ggtcaggaaa tttattgact aaaccatgtt ccactaatct
     8221 gtcaataatc ggagtcatat ttgattttaa aattcctaaa ttagaagcta tttgagaaat
     8281 tgatgatgaa tttgatgtca taagataaag aattacctta gcatgtgatg gagccattgg
     8341 acattcttca aaataagctt tcattatatc agagttattt tgaaactttt ttaacaaatc
     8401 gctttcttta aatatattat tttcaagcac aaataaaaaa tctaaaaaag agtccgctat
     8461 tttaagtata ttgt
//
LOCUS       DAEPOZ010000161         8469 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.161, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000161 DAEPOZ010000000
VERSION     DAEPOZ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8469
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.161"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            155..1630
                     /locus_tag="KR440_002699"
     CDS             155..1630
                     /locus_tag="KR440_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBH0699734.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            1717..2130
                     /gene="speD"
                     /locus_tag="KR440_002700"
     CDS             1717..2130
                     /gene="speD"
                     /locus_tag="KR440_002700"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBH0699735.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            2160..3011
                     /gene="speE"
                     /locus_tag="KR440_002701"
     CDS             2160..3011
                     /gene="speE"
                     /locus_tag="KR440_002701"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBH0699736.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            3001..3879
                     /gene="speB"
                     /locus_tag="KR440_002702"
     CDS             3001..3879
                     /gene="speB"
                     /locus_tag="KR440_002702"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBH0699737.1"
                     /translation="MKNNFYHMNTFMSMDKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(4059..4259)
                     /locus_tag="KR440_002703"
     CDS             complement(4059..4259)
                     /locus_tag="KR440_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBH0699738.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
     gene            4726..6216
                     /locus_tag="KR440_002704"
     CDS             4726..6216
                     /locus_tag="KR440_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBH0699739.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
     gene            6474..7970
                     /locus_tag="KR440_002705"
     CDS             6474..7970
                     /locus_tag="KR440_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBH0699740.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACISACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFEFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            8104..8454
                     /locus_tag="KR440_002706"
     CDS             8104..8454
                     /locus_tag="KR440_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0699741.1"
                     /translation="MKKIVLKDILFLVTTLSIIVILLFVTNITTNSKIKSTSESYLKS
                     DKNSVRQNGIQRSTKNTAFKTTILSTGKSDCILIEIGDKFIMIDTGEDKNGEQIVDKL
                     KEKEINALDYLIKK"
BASE COUNT         3373 a          900 c         1539 g         2657 t
ORIGIN      
        1 gtttttgagt aacttcaaaa aaacacttgt aaaaatgctg gaatgttaat ataataaata
       61 tcatgagttt aataaaagta aaaataaaaa atagaaataa taaataataa atattaaaaa
      121 tatttgaata tagagaggag atgaatttga aatcatggat agcgagtttg caaatgcagt
      181 tgaattaagc tatgagtgtg ctccattatt agagtcgtta aaggaatact cagagaaaga
      241 tatagcttgt tttgatgttc ctggacatgt aaaaaataga ggtgtagcta tattaaataa
      301 atatctaggt gaaagtctca tgaaaatgga tataaattca tcaccaacta tggataatgt
      361 atctgcgcca aatgggatta taaaaaatgc acaagatttg ctagcacaag catatatggc
      421 tgatgaggca ttctttataa cgaatggcac aactcaagca atacatgcaa tgattttgag
      481 tgttataaaa ccaggtgaaa aagtattact tccaagaaat atacataaat cagtaataaa
      541 tgcgttaata ctttgtggag gaataccaat atttatacaa ccagaatttg atgaaaaact
      601 aggtataagt ttaaatatta ctcttgaaaa agttagaaca gagatagaaa aagattgtaa
      661 tataaaagca ttatttttcc ttaatccaac ttactatgga gtttgtgcag accttgagag
      721 tattatagaa ctatgccata aaaataatgt tttagtgtta gttgatgaag ctcatggtgc
      781 acattttcca tttcatttag atttacctcc ttctgcaata agtttaggag ctgacatggt
      841 agctgtaagt atacataaaa ctggaggagc attgacacaa tcttcagcac tcttattgaa
      901 tagagataat gtaagttttg aaaaggttct tcaatcaatc aatatgctac aatcaacatc
      961 agcatcttat ttgcttatgg caagtataga tggtgctaga gttaatctag ttgagaatgg
     1021 tgaaaagcaa ctgtcaaaag cattaaatct atctaggtat gcaaaatcta aattgaacaa
     1081 aattgatgga ataaaagttt tatctacaga aatattgaaa caaaaaggtg ttaaatttat
     1141 agatgaaaca aaattgtgta taaatgtaaa agaattaaat ttgactggat ttgaagtata
     1201 cgatttatta tataaaaatt tttcaattca agtagaattg ggagattcat ataatatact
     1261 ggcacttgtg tccattggaa caaataaatc agatatagat agattagtaa aagcactttc
     1321 tataattgca aagatttata gaaaagaatc aacattaaat gaatttaata tggttcaaat
     1381 aaatccaatt attaaactta atccaagaga agctttttat gcaccaaagg aaagtgtaga
     1441 aatcaattca tgtatagata gaatttgtgg agaatcaata atggcatatc caccaggtat
     1501 accgattatt gctccaggag aattgataac agaagaaatt atggagtata ttattttctt
     1561 aaaaaatagc aatgcgtatc ttacagatgt acaagataaa aacttagata gaatactagt
     1621 aataaaataa attactttat tttgcaaggg caagtgtttg ttaagtagag tttatcaaat
     1681 agaacttatt aaaagtataa aggagttggg gttgaaatga aatttgaaac actaggaaga
     1741 catatactag tagaatacta taattgtgat gaaaacgtac ttaaagaccc acgtcttata
     1801 gaaaatttta tgaatgattc agcattaaat gcaaaagcga caattgtgga ttctgtattc
     1861 caccatttta atccttgggg ggtttcagga gctgttataa ttgctgaatc tcatttgaca
     1921 atacatacat ggcctgaata tggttatgca gcagcagatt tttttacttg tggtgatata
     1981 gacccatgga aaagttttga attattagag caacttttaa agtctgaaag aagcgagtct
     2041 acagaaattc caagaggctt aactacaaaa attaaaaaat attcaaaaaa agatttaggt
     2101 aagattactc ataaaccaga agcagtataa aacatacaag agaaagtgag gattattata
     2161 tggaactttg gtatacagaa gaatggacag aaaatgtaag attttctata aaagtaaata
     2221 aacatctatt tgaaggaaag tctcaatttc aaaggataga tgtatttgat agtgatgaat
     2281 ttggaaaatt tctaacaata gatggattaa tgatggtaac atataaagat gaatttatat
     2341 accatgagat gataactcat gttcctatgg ctacaaattt aaacataaag aaagtattag
     2401 tcataggtgg aggtgatgga ggtacagtaa gagaattaag ccgttatcct caaatagaaa
     2461 agattgacat ggttgaaata gataaaatgg ttgtagatgt atctaaggag tatatggata
     2521 tatgctcatg taaattagat gataaaagag tgagtttata ttttgaagat ggtgttaatt
     2581 ttgttaagtg tgcacatgat aaatcatatg acctgataat agtagattca actgacccaa
     2641 taggaccagg cgaagggctt ttctcaactg atttttataa ggattgttat agaatactga
     2701 ctgatgatgg aattttagta aatcaaagtg aaagtccgta ttttgacttt aatgctaaag
     2761 agatgaaaag agctaataaa aaattaaaac agatatttcc aatatcagaa gtttatcaag
     2821 cacatatacc aacatatcca tctggtcact ggttgtttgg atttgcttca aaaaaactta
     2881 atccagtaaa aaatcaagat agaaatggat gggagaaatt aagtttaaaa actaaatatt
     2941 ataatagtga tatacattta ggttcgttta tgttacctca atacgtgaag gagatgctag
     3001 atgaagaata atttctatca tatgaacact tttatgagta tggacaaaaa ttatgaagaa
     3061 tctaatctta tagtatttgg tgttggattt gatggaacta cttctaacag acctggggct
     3121 agatttgcaa gtagctctat gagaaaagaa ttttacggtc ttgagacata tagtcctttt
     3181 ttagatttgg acttagagga ttataatata tgtgattatg gagatttaga aattagtgtt
     3241 ggaagtacag aacaagtctt aaaagaaatc tatcaagaga catataagat tgttagagat
     3301 tcaaaggtac cctttatgat tggaggagag catttagtta cattaccagc ctttaaagca
     3361 gtacatgaaa agtacaatga tatatatgta attcattttg atgcccatac tgatttgagg
     3421 gaagaatata ataatagtaa aaattctcat gcaacagtaa ttaaaagaat atgggatatt
     3481 gtaggtgata ataaaatatt tcaatttggt ataaggtctg ggacaaaaga agaatttaaa
     3541 tttgctacag aagaaaaaca cacatacatg gaaataggag gaatagatac gtttgaaaat
     3601 atagttaata tgctaaatgg aaagaatatt tatctaacta tagatttaga tgtattggat
     3661 gcatctgtgt ttccgggaac aggtacacca gaacctggtg gtgtaaatta tagagagttt
     3721 caagagattt ttaagattat aaaaaactct aatataaata tagttggttg tgatattgta
     3781 gagttaagcc ctgattacga tacaacaggt gtatcgacag ttatagcttg taaaatctta
     3841 agagagttat gcttaataat atctgataaa attaaataga gtattttata taaatagtat
     3901 atattataat ataagaagtt attttaaaaa agttagttta agtttgacgc ttttctaaag
     3961 taacttctta tttttgtaat taaaaataaa tataataaac aacaaaaaag agccctcgat
     4021 agctcctttt ctaaaatata tatatttaaa ttaaataatt atattctatt tacgttttca
     4081 gcttgaggtc ctctattacc ttgagttata tcaaagctaa ccttttcgcc ttcttctaaa
     4141 gttttgtatc catcattttg tatagctgag aaatgtacga aaacatcatc ttctccctct
     4201 acagatatga acccaaatcc tttttcattg ttaaaccatt ttactattcc gtttttcatt
     4261 ataagaaaac ctccgcatta ttaaaaaata caaactaaaa aaataagtct gttctattaa
     4321 tataacataa attttaaata aaagcaaata aaatttttaa aaaaattaat tacttttaga
     4381 atttaataaa aattgttaaa ttatacagtg agaagtattg taaaatttga cgaaaatgta
     4441 aatgtagata ataaatttaa tgtcaaaaaa tgcaacaaaa aggtatgtaa gtaaaaagta
     4501 aaattaaaat atataataaa aaatattata tattagatta aaaagtaaaa ataaaaatag
     4561 tgaaatttca gaaatatcaa taatattata taataaaata aaaaaacact atatatattc
     4621 gataaaataa aatttaaaca tataatattt tatgtatata ttaataaaag gacatattgt
     4681 gttataatgg tatcgtataa aatcatttac gggggtggta ttaaaatgtt tcaatttgaa
     4741 acacaaatac acaaattaag acatgatgtt ttaactgcaa tagctagact tggaaaagaa
     4801 gataacctta ccagagaagc aatggataat attcagtatg aaataataaa aggtgagaaa
     4861 cctacataca gatgttgtgt ttataaagaa agagctataa tatctgagag agtaaatgta
     4921 agtatggggt tacaaccagg tcaagataag atagatttag atacaatgga tatagaagaa
     4981 gatgaacaaa ttatatatat tttggaagct gcatgtgaca gttgtccaat aaatgaattt
     5041 acagtaacag atatttgtag aggatgtcta gcacatagat gtaaagaagc ttgtaaattt
     5101 ggagctataa cacatgtcgg aggaatggcc tatataaatc atgaattatg taaggcttgt
     5161 ggaatgtgta aaaaagcttg tcaatatgat gccatctcag aggttgttag accatgtaag
     5221 agtgtttgtc caactaatgc cttaggattt gatagagaaa atatgaaagc tatgatacat
     5281 gaggataaat gtttaaactg tggagcatgt atgtcagcat gtccatttgg agcaatatct
     5341 gataagagtt tgatagctcc tgtagctaga aagttggttc aaaaagagaa aatgtatgcg
     5401 gtagtagctc cagctataac aggtcaagtt gaagcaaatg ttacatatgg tcaaataaaa
     5461 aatgctataa aatcactagg atttgttgat gtattagaag cagcttgtgg agcagatgca
     5521 gttacagttc atgaaagctt agaatttgct gaaagaatgg aaaagggaga ggcttttatg
     5581 acaaattctt gttgtccagg atttgttggt tatatagaaa aaacataccc aaatcatgta
     5641 ggaaaaatat cttctacagt gtctcctatg atagctagtg gtagatttat aaaaagtcat
     5701 gaaccagatg ctaaagttgt attcatagga ccatgtacag ccaaaaagag tgaggcagct
     5761 aagaaagaat taaaaggagc tgttgattat gtacttactt ttgaagaaat aatggcatta
     5821 tttgaggcat ttgatataga cttggctaag tgttcaaatg aagatataga tgatgcatca
     5881 atttttggaa gaggttttgg agctgctggt ggtcttacaa aagctataga aaattatctt
     5941 gctgaaaaag gtattggtgt taattttgaa cctgtaaaga tttctggttc aagagaaatc
     6001 aagaaaacta tgactatggc taatttagga aagctagaag gtaattttat agaaggaatg
     6061 atgtgtgaag gtggttgtat aaatggttct ggtaaattct tatcaggtgc aaaggttaaa
     6121 gccacttttg atagaaaaaa ttctcaatca actaagaaaa gtgtcttagc aaatacaaag
     6181 ataaaagatt ttagtaatat aaatttagaa aaataaagat aatacaaagc aacaaaaata
     6241 catattgtta atagagttgt tctaagttga ggtttaactt ttggacaact ctatttttat
     6301 ttatatattt aattaatgaa caactaaaat aaaaattaca tatataatat tttgtatata
     6361 acttaacaaa gtatatgcct atgttataat aatttataga aataaataaa aaatacaata
     6421 tacataatag tagaaatata gtaaaaaggt gtgatttata gggaggatta cttatgtttg
     6481 aatttgaaac gcaactaaaa aggttgaaac atgaagtttt aacagcagta gcaagactat
     6541 ctattgaaga aaatttaaca aaagaaaata tagaaaaaat tccatatgaa ataattaaag
     6601 gagaaaaggc gagatataga tgttgtgttt ataaagagag agaagtagtt cttgaaaggg
     6661 cgcaacttgc attaagttta actccaaata gtaaatatgg agatataaat ccagattcag
     6721 ttagtttgga cacagatgag caaattatat atatagttga agcagcatgt gatagatgtc
     6781 caataaatga atttacagtt acagagcttt gtagagggtg tttggctcat agatgtaagg
     6841 aagcatgtaa atttggggca atttcttata taaatgggag agcatatata aatcatgatt
     6901 tatgtaaggc ttgtggaatg tgcaaaagtt catgtcaata tgatgctata tcagaagttg
     6961 taagaccttg taaaagcgtt tgtccaacag gagctttgga ttttaataga aacactatga
     7021 aagcaatgat acatgaagat aattgtataa attgtggagc atgtatatca gcatgtcctt
     7081 ttggagcaat atctgataag agtttgatag cacctgttgc aaaaaaactt gcaaagaaag
     7141 aaaatatgta tgcaatagta gctccagcta taacaggtca aatagatagt aatattacat
     7201 atggtcagtt aaaaaatgct ataaaatcac ttggatttgt agatatgata gaagcttctt
     7261 gtggagcaga tgcagttaca gttcatgaaa gcaatgagct tattgaaaga ataaaatcag
     7321 gagataatta catgacaaat tcatgttgtc cgggattttt taagtatata gagaagatgt
     7381 tcccagatga aaaagataga atatcatcta cagtgtctcc tatggtagca acaggaagat
     7441 tagtaaaaaa aatagattct gatgctaaag ttgtatttat agggccatgt acagctaaaa
     7501 agagtgaggc aacagagaaa agtttaaaag attcagtaga ttatgtactt acttttgaag
     7561 aactaatggc attatttgaa gcatttaata tagatttatc aaaatgttca aatgaagata
     7621 taaatgatgg ttcgattttt ggaaggggat ttggagcgtc tggtggactt gccaaatcag
     7681 tacagaacta tattgctgaa aagggactta gttttgagtt tgaacctata aaagtatctg
     7741 gtccaagaca aattaaaaag acaatgacaa tggctaaatt gggaagatta gaaggtaatt
     7801 ttatagaagg aatgatgtgt gaagaaggtt gtattaatgg agcaggtaag tttttatcag
     7861 gtgtaaaagc taaaaatact tttgatagaa aaaatggaca atctactaaa aagagtgttt
     7921 tatctaatga taagattaaa gaatttgaag atgtaaattt agaaagataa ttgttttggt
     7981 acagtcattt ttttatcatg ccacaaatat gcctaaaatt ttcccttttt atatgtgtat
     8041 tttacagagt taaggttgct atactataaa tatgtaataa ataaaattta taggagcttt
     8101 tggatgaaga aaatagtatt aaaagatatc ctgtttttag ttactacatt atctataata
     8161 gtaatattat tgtttgtaac aaatataact actaatagta agataaaatc aacaagtgaa
     8221 tcttatttaa aaagtgacaa aaatagtgtg aggcaaaatg gtatccagag gagcacaaaa
     8281 aatactgcat ttaagactac tatattaagt actggaaagt ctgactgtat attaatcgag
     8341 ataggtgata aattcataat gattgataca ggagaagata aaaatggaga acaaatagtt
     8401 gataaattaa aagaaaaaga aataaatgca cttgactatt tgattaaaaa atagctacgc
     8461 acactcgtg
//
LOCUS       DAEPOZ010000162         8441 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.162, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000162 DAEPOZ010000000
VERSION     DAEPOZ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8441
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.162"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..1634)
                     /locus_tag="KR440_002707"
     CDS             complement(<1..1634)
                     /locus_tag="KR440_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBH0699742.1"
                     /translation="MMFKIFNKSLLKLVIVLSIVLTISNFSLFSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINENYYSHIFILGINENS
                     YNNDKNTKNLISSLNSYKVTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYMGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLDKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     ITKSDTRFKYISNISKILIFIVSISVLIFVIIFIYF"
     gene            complement(1624..2472)
                     /locus_tag="KR440_002708"
     CDS             complement(1624..2472)
                     /locus_tag="KR440_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH0699743.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            complement(2916..3968)
                     /locus_tag="KR440_002709"
     CDS             complement(2916..3968)
                     /locus_tag="KR440_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HBH0699744.1"
                     /translation="MIKFKKTISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            complement(3968..4642)
                     /locus_tag="KR440_002710"
     CDS             complement(3968..4642)
                     /locus_tag="KR440_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0699745.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            complement(4747..5583)
                     /locus_tag="KR440_002711"
     CDS             complement(4747..5583)
                     /locus_tag="KR440_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0699746.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            complement(5585..6628)
                     /locus_tag="KR440_002712"
     CDS             complement(5585..6628)
                     /locus_tag="KR440_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0699747.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            complement(6647..7186)
                     /locus_tag="KR440_002713"
     CDS             complement(6647..7186)
                     /locus_tag="KR440_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0699748.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            complement(7622..8311)
                     /locus_tag="KR440_002714"
     CDS             complement(7622..8311)
                     /locus_tag="KR440_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699749.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADS
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
BASE COUNT         2836 a         1467 c          814 g         3324 t
ORIGIN      
        1 ttaaaatata taaatataat cacaaatatt aaaacactta tagatactat gaatattaaa
       61 attttactaa tattggatat gtatttaaat ctagtatcac ttttagttat ttcatctaag
      121 tccttatcat agttgactat aatttcatct ccattatttc ttatttgtat ttcatcaact
      181 tttacccaat tattaaaatc tcttaaattc ataaattcta tattctgttt ttctaaatat
      241 tctattgtgt tttttagata ctctatgttt aaatatgaat gaaagaagaa ccctccacta
      301 aaacctctta caatagataa tttatctaaa ttttctttta tattttgaaa agtaaacttg
      361 tcttctgggt ctatatatcc taaattttcg ggtataaata tattaaactc ctctgtatcc
      421 ctaataatat aaggatatgt atttgtgcta aattttttat cattattttg atgttgcccc
      481 atataagtag aaaaatattt ttttaattct ttatatccct ctgattccat tgcataatgt
      541 ggcgcttcaa atgcaagagg ataaattcca ttctctatac atactctaag accattcaaa
      601 actctatctt tgacaaatat cttcatattt tcttttaagg gttcatcttt tttaccatcc
      661 caaaattcat acccttctcc agaaacttct tctttcttgt attgatgagt atatccatgc
      721 aatatcacag ttccaccttt atcttgcata tattttattg ctttgacaat ttcagggctt
      781 tctgataaag ttattacttt atgattttta ggatttacat atgctggtat taatgcaatc
      841 gtaaatggta tattcttact acttaaataa tccgctatct ctacaagatt ttttggttct
      901 ctaaaagcat gtacatcttc tattcttaca aatatacgtc ctttatcaaa cgtcttaata
      961 ttaaaaatat cattaagtga atcgcaaaat atataaaata aaactccatc taaatctaat
     1021 tttgatacat aaaaaagatt tttatcattt attatatatg gatacttatt taaggtatca
     1081 ttaatacttc ctattacttt attacttgta tcttttgatt ctacaatgtt aaaaacataa
     1141 tgttcatcca agttatatga tttacctcta taatttacac taactatatt gtttgttttt
     1201 cctacatagt ctaaattgta ttttttatgt tccaaaagat tttctatacc atatcctaac
     1261 caacatatag ttactttata tgaatttaag gaactaatca agtttttagt gtttttatca
     1321 ttattatatg agttttcatt gattcctaaa ataaatatat gactgtaata attttcattt
     1381 atctcaccat catagttact caattttaat agctctatat ctgaactaaa tcttcccaat
     1441 agagttctca taatatttaa aatatctcta ttataagctg tttctttctt agaatcatat
     1501 atgattaata ccttatctgt gctggaagta atttctgatg catatgaaaa taagctaaaa
     1561 tttgatattg tcagtactat tgataataca attactaatt ttaataaact tttattaaat
     1621 attttaaaca tcatattgaa gttcctcttc aactatattc ttaaaattta tactatctcc
     1681 aaaactactc ttatattgta aaaatgcaat tttgacatct atatttacat attttcccct
     1741 attgtttaaa tctaaattta attcttttat ccttttcttt attctgtctt taacaacttt
     1801 cgaaccttcc aaagatgtat ttggcattag tatccctatc atatcttttt gtaaactata
     1861 aattatatct tcatttcttg tacattctat aatatttgaa ccaatatatt ttactatttt
     1921 attagttttg ttttcaccaa atatagtctg taggtttccg tagtatggta attttactat
     1981 cataagagaa aaatcagtat tatgtctaat agatttactt atctccatat ttacatcatt
     2041 ataaaaaatc tttaaatttc taagtccagt ttcactatca atagtcacta attccttata
     2101 ctgttcagat agttttttat ttgtattttg caattcattt atgcttttgt tcagattacc
     2161 cattataatt gaagatactg gtgttgctat tatccacata tatgatataa attcaacatc
     2221 catattgtga gtaatattgt tatataaaat ataggatgta tacataaata taattattga
     2281 acttgttatc aaccccacta ctgaatttga taaataagtt aacattatta taaaaaaaat
     2341 tattgctaac attatgaaca cattcaattt attttctgtc ctaatcgata gtattactat
     2401 tccaacaatt aaaaatataa aaacgagcaa agccaatatt aataaatcta tttttttatt
     2461 tatcctattc attttttata aatttacacc tccaattatt ctattaagtt gcccattttt
     2521 tctaattatt attaaaatca ttatacaaat aagttttcat ttattaataa actatttaaa
     2581 ttgcatatga atataactta ataaaaaata ctgtctcaaa gcaaattctt tagctcatga
     2641 atcactcttt ttttatgaaa tcaaacatgt tttcatcatt tcaacaatga aaaagaggct
     2701 tatctcaatt tcgagacaac ctctttatag ttattattat aaaaatagta atcaatgaaa
     2761 cttactgtac tttaaattct cataaaaata aactcaaaat aaaaacatga cctaaacaaa
     2821 aaataaaact gttcaataat tagaaaataa attttctaaa aaagaacagt tttattaagt
     2881 atttattaag taaatttact taaaccaaca agaccctatt ttgattttaa ctctatccaa
     2941 gccttgtcat ataattttat attttcacca atatcttcaa aagtttcaca tttatcaagt
     3001 acttctttag atggatatgc aacaggattc tgagttattt ctgggtctaa taattttagt
     3061 gctcctgtat taggtgttga atacccaatg tactcagcat ttacttttgc attctctggg
     3121 tctaataaaa agtttataaa cagctcagct tctttcttat tttgtgctgt tgtaggtaca
     3181 cacatagcat ctacccattt attagttccc tctttaggta tagcataatc aaggtcagga
     3241 ttttgctcca ttagcgtaac tgcatctcca gaatacagca tacccattgc cacttctcca
     3301 ccaagcaatc tatccttacc ttcatcattt acataagcca atactaaatc cttttgtttc
     3361 attaaaagat ttttagcttc atttatttct tttggattta cactattcat gctatatcct
     3421 aattttttaa gagttatccc tatagtatct ctaacagagt caaacatcat tatatttccc
     3481 ttatactttg gattccaaag tatattccag ctatccatag ggtctttaac ttctttctta
     3541 ttgtacaata ttccaagagt tccccacata tatgcaacac tatattcatt agttttgtcg
     3601 tatacaggat ttaaaaaact cttatctaaa tatttcatat taggtatatt cttaaaatct
     3661 aatttttcca aaagaccttc atttttcatt ttctctacca tatagtctga aggaaatact
     3721 aaatcatagt gtgtactacc actctttact ttttgataca tcatttcatt agtatcataa
     3781 gttgaatatt gaacatctat tcctgtttct ttttcaaact tatctataag agactcatct
     3841 atataatctc caacattata tacatttaaa acttttgtag aatcagttgc tttattacat
     3901 cctgtaaata aagtaactgt catcatacct acagctataa gggatattgt ttttttaaac
     3961 ttaatcatta tttaatcccc cttactatag attctttttt attagctagt aaaagtaaac
     4021 ctagaaccac tgcaaacatt attgttgata atgcatttat ttcaggtttt atacctcttc
     4081 tagccattcc atagatttct atagaaagat tgcttacacc atttccagca ttaaagaaac
     4141 ttattataaa gtcatctatt gacatagtaa atgcaattaa aaatccagaa actatacctg
     4201 gttttatttg tggtaaaatt acttttctaa gagcgtatat aggtgttgct cccaagtcca
     4261 tagctgcatc ttctatatta tcaggtaatt gcttcatttt tggcaaaact gaaagtataa
     4321 cgtatggcaa acaaaacatt atatgtgcca atagcattgt tgtaaatcca aattccattt
     4381 ttacaaatac aaacaggctc ataagagcta ctgctgtaac tatctctgta tttaaaatag
     4441 gaagatagtt tacatttagt attaatttct tactcttacc tctcatctta tgtattccta
     4501 tagcacttat agttcctact attgtagata ttacagacgc taatatagct attacaatag
     4561 tataatataa agcactcatt atactctcat tttgaagtaa ttgaccatac cacttccatg
     4621 taaatccatt ccatcttgcc attgattttg aatcattgaa agaaaatagt accaaagcaa
     4681 atatgggtgc atacaaaaat aagaatacta atgctaaata tatattagaa gttattttct
     4741 ttaattttaa aatagtaacc caccgccttc tttgtcagat tcatctccaa attttgacat
     4801 tatagacata gatattaata ttactatcat cataaatatc gatatagctg aaccaaagtt
     4861 ccaatctcca acaactgtaa actgttgttc aatcaaatct ccaaccagca ttattttacc
     4921 tccacctaac agtcttgata ttgcaaaagt agtgactgct ggcataaata ccatagttat
     4981 tccagacatt acacctggca aactcaaagg aaatataatt tttctaaaaa ctgtcatatt
     5041 atttgcaccc aagtcatgag cagcgtttat caaatcatta tccatctttg atagtgctgt
     5101 atatattgga agaaccataa atggtaaaaa gttatacacc atacctaaaa gtatagcaaa
     5161 attagtatac aaaattgcta atggttgtat tccaaaccaa cctaaaaatg tatttaaaag
     5221 tccatttttt cctaatatag caacccatgc atatgttctt aaaagaaagt tcatccacat
     5281 aggaagaata aacaataata ttaatgaacc tcttctactt acacgagcct ttgaaattat
     5341 atatgccact ggataaccaa ctataaggca tattaatgta gctcctcctg ctaaaagaat
     5401 agaacgccca aaaactttca tatatatagg gtcaattact tctttataat tctcaagtgt
     5461 aaaggcataa cctccacctg attcttttgt aaaactaaaa aataccacta atattaaagg
     5521 aattattaca aatatcgcac tccaaactac atatggatat gctaaaaaag attttctttt
     5581 catcttagtt attctctttt ctcattatat ggatatcttc tggatatata tccatgccta
     5641 gttcagtccc aacctcagca tttttggtat tatgaagtat ccacaatctt ccattttcat
     5701 tcaattctat ctcgtaatgc actcctttaa acactgtaga tgtaactttc ccctttaaca
     5761 ttccttcttc tggctttacc atttttatat cttctggtct tattactact tctatatttt
     5821 catttttttc gaatccttta tcaacacact caaaatctct attacagaaa ttaactttat
     5881 aatcttctat cataattcca tcaaatatgt tactttcacc tataaattta gctacaaatg
     5941 aatttttagg ttcattgtat atatcttgtg gtgttcccat ttgttgaatt ttgcccttat
     6001 acattacaac aatagtatct gacatagtaa gagcctcttc ttggtcatga gttacaaaaa
     6061 caaatgttat tcctaatttt ttttggattc tctttaattc tatttgcatc tcttgtctta
     6121 actttaaatc aagtgctcca agtggttcat ctagtaataa tacctttggt tcattgacta
     6181 gagctcttgc tattgcaatt ctctgttgtt gtcctccact caatgattca acttgtcgtc
     6241 tttcaaaacc ttctagtgca acaagtttca aaacctcttt tactttagca tctattacat
     6301 cttttggagt ctttttaatt tttaatccaa aagctacatt ttcataaaca tccatgtgtg
     6361 gaaataaagc atatttttga aatactgtgt ttacttgtct ttcatatggt ggcaagttat
     6421 ttatctcctt accttcaaat aaaactttac catcatcagc atactcaaat cctgctatta
     6481 tttttaatgt tgtagttttt ccacatccac ttggtccaag taatgttaaa aactcatttt
     6541 tctttatatc taaattgaaa ttatctaaaa cagtaagttc ctcataatgt ttagatatat
     6601 cccttaattc tattatattt tctaccaatt attaaacacc tctattctaa aatgatggtg
     6661 gcgtacttac ccaaatcact tttgctgtag ttttgccttc gtttgatata tagtgatttg
     6721 cttttggtct aaagtaaaaa ctctctcctt tttttacttt atttttcttt tcgcctatat
     6781 ggacatatat tgaacctgct aatacatatc caaattcttc accttcatgt ggcttttctt
     6841 ctttatactg tcctcctgga tacaatgtta ttattattgg ctccatttca tttttttgtg
     6901 aatttggaat taaccacttt aatttatatt ttaaatcttc atcttcagtt tcaaacatat
     6961 cttcttttgt aaaactgatt ctttcatcat ctatttcatt aaaaaattcc cttaaatttg
     7021 tacctagtat ttcaagtata tctataaggg tagctatgga tggtgatgtt aagttatttt
     7081 ctaattgaga aataaatccc tttgacaatt cacatctatt agctagctct tcctgggtca
     7141 gttgtttttc tgttcttaat ctttttatct tttcacctat atccatgttg cacctcctag
     7201 tttctgtttt attttactaa acattttgtt tactaaacat tgagtttaaa atttctaaac
     7261 aacataggta attatatata aaatttggat ataatacaag agtttttttg acattttttt
     7321 atttttttat ttagaataaa tacaagcatt ttaaaggctt tggaaaatac acagtttaaa
     7381 ggtgtttata ttaatatcta taaagtttag tatctctaat tataaattaa ttcattcatt
     7441 aaatagagaa taagacttct agtactaaaa aaacattgaa caaataaaaa attgctagaa
     7501 atctatattt tatagtgttc tagcaatcct catattttaa tattctttta atattcaatt
     7561 tatccatcta aataaaatgt taatttaaaa tacttaacat ttcgttgaat ttattcttaa
     7621 attacttaac tatattttga acagcagctt ttttctcacc actcatatac actttgataa
     7681 attttatatc atctactata aatctatgtt taatcttact acaatctaat ttaaataagc
     7741 caaataccat ttgtgtgaaa taccccaatc caaatgcact tattatagtt cctataccaa
     7801 cttttccacc taataaaaat cctactatta atgctccaac ttctatagta cctcttatta
     7861 atttaagtga ttttcctgtt ttcttttgaa ttgcaaccat caaaccatct ctaggtccac
     7921 ttcctaaacc acttcccata taaaatacca ttccaacaga caaaagaacc aatcctaata
     7981 tcaacataat tattcctaca aataaactat cagcctctgg aattaaatta ctatataaaa
     8041 gtaaatccat aaagaatcca attagtagca tgttaaaaac agttccccat cctacatttt
     8101 ctccaagaac gacatcagca ataactacta tagagcctac aattatagaa gcagttccta
     8161 tagttattcc tatatgattt gaaagtcctt gatgaaatac atcccaggga gataatccaa
     8221 ggttacaatt tatcgtcaat actataccaa ctgaacatat aaataaaccg actactagct
     8281 ttaaaaattt ccataatact ttttgcaaca aattctaccc ctcctaaaca taatttaact
     8341 actcactcta aatatcaaaa gccagcagtt ttcttacaaa aatcttattt tattaatttt
     8401 ataatatact tctatgatac attattttat gctaaataac t
//
LOCUS       DAEPOZ010000163         8322 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.163, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000163 DAEPOZ010000000
VERSION     DAEPOZ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8322
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.163"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            38..754
                     /locus_tag="KR440_002715"
     CDS             38..754
                     /locus_tag="KR440_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBH0699750.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            888..1580
                     /locus_tag="KR440_002716"
     CDS             888..1580
                     /locus_tag="KR440_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0699751.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            1570..2751
                     /locus_tag="KR440_002717"
     CDS             1570..2751
                     /locus_tag="KR440_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBH0699752.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKLLYFNSEI
                     SVINQLVYQLRDFRSLIGESLFYPFLFTITSLAIYSMISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKMEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            3033..3488
                     /locus_tag="KR440_002718"
     CDS             3033..3488
                     /locus_tag="KR440_002718"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBH0699753.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            3478..4203
                     /locus_tag="KR440_002719"
     CDS             3478..4203
                     /locus_tag="KR440_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HBH0699754.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIRINKDNVVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLNQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            4175..6556
                     /locus_tag="KR440_002720"
     CDS             4175..6556
                     /locus_tag="KR440_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HBH0699755.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDNAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSCGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(6761..7990)
                     /locus_tag="KR440_002721"
     CDS             complement(6761..7990)
                     /locus_tag="KR440_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBH0699756.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
BASE COUNT         3319 a          927 c         1456 g         2620 t
ORIGIN      
        1 ttatagacaa acataacaaa aaggagaaga tttaattatg aaaaaattta aattaatagg
       61 agccattatt ttagtagtat ttataagtgt atttatatct aaagatagat tacttaagaa
      121 agataaagat ccaatagtag ctgctcaaca tgattcagaa caagatgttg aggcaaattc
      181 tcaaaataaa aatcactcaa atagtatatc aaatatatta ttggtaaaca agacaaatgg
      241 aattagcaaa aactatactc ctgaaaatat tactaaggta aacatacctt ttgttgaaga
      301 agcaacagag gaagaaaaac aaatggcagg agaacctgca aaggcagttg aagatttagt
      361 taagcaagct aattcagaag gaatacagtt tttagggtct tcagcataca gatcttatga
      421 tactcaactg gatacttata caagaagggt aaaatctcag ggacgagaaa aggcagatgc
      481 atatgtagca aagcctggtt acagtgagca ccaaactgga ctttgtattg atttaacaaa
      541 tccagaaaga tggtttgttg gaagtacaaa agaagctaaa tggttggcag aaaatgctca
      601 caagtttgga tttataataa gatatcctga aggtaaagaa gatataacag gtacagcata
      661 tgaaccatgg catattagat atgttggtaa ggatgctgcc gaggaaattt attcaaaagg
      721 attaacttta gaagaatatt tacaaaataa ataatataaa tgtgtaaata atttgtatta
      781 tattggaaat ttagaaatga ataaatttat agattacact aactaaaata tagtataatt
      841 ttatatgaat aaagctatat acaaagatta agttaggatg tgtaaaaatg aataatacag
      901 ttcttgttgt ggatgatgaa aaggaaataa gagaccttat agagatatat cttttaaata
      961 gtggatataa tgtaattaaa gctgaagatg gaaaagaggc tctagatatt ttaaaaaagg
     1021 aaaatattca tttaatgata ttggatataa tgatgcctaa aatggatggg atagaagttt
     1081 gtagaaaagt tagagaaagt ttgaatatac caatacttat gttaagtgca aaatcagaag
     1141 atatggataa aatacaaggg ataatgactg gtgcagatga ttatttgaca aaaccattta
     1201 acccacttga acttactgtg agagtgaaat cactgattag aagagcatat tattttagtg
     1261 gtgctaataa tgaagataaa gatgtcataa aggttggagt agtaattata gataaggaaa
     1321 aacatagtgt aacagtggct ggaaatgaaa taattttgac atcaagagaa tttgatatat
     1381 tatatttact agcaaataat aaaggcagag tatatagtac agaagaaata tttgaaaagg
     1441 tatggaaaga gaaatattac caatctaata atactgtaat ggttcatatg agcagaataa
     1501 gggataaaat tgaaaaatat acagatggag aaaaaataat tcatactgta tggggagtag
     1561 gatataaaat tgaaaaataa aagtttatta aatcatatgt ttcgacccat attagatctt
     1621 ataataagct gtgctttaac ttcaatgctt attttaacaa gtatgtatat aataaaatta
     1681 ttatatttca atagtgagat atctgtaatt aatcaacttg tatatcagtt aagagatttc
     1741 agaagtctta taggagagag tttattttat ccatttttat ttacaatcac atcattagca
     1801 atttattcca tgatatcgta caagagaaat aagcaaattg tagattttat aagaaagact
     1861 aatagaaaaa atcaaaaatt agaaatcatt aaaagtgctg taaatttaat ggatgaaggt
     1921 aacttagaaa aaagtataga tattgagtat gatttagata tattaaagga taaagaaaag
     1981 gatgatattg atgaactagc acataaaatc aataatatag tccatcaact tagaaatata
     2041 actattgaag agagacaagc tcagcagaca aaaacagatt taataacaaa tgtgtcacat
     2101 gacttaagaa cacctttgac atccataatg ggttatttag gagtgataga agaaggtaag
     2161 tataaagatg aagttcagat gatgtattat gttgatattg catatgaaaa atcgaaaaat
     2221 ttaaatgttc ttataaatga tttgttcgaa ttgactaaaa tgcaaaataa tacaataaaa
     2281 ctaaacaaaa tagaaataaa tttagtagag ttattaagtc aagtagtatc tcaatttgaa
     2341 atttatttta aacaagaatt tatggttagt agaattaatt ttaatgaaga aaaattaatt
     2401 gtgaaagctg atccaaataa attggtaaga gcatttgaaa atcttataac taatgctgtg
     2461 aaatatggaa aagatgggca ttatgtagat atagtcacag agaaaatgga agatatggcg
     2521 gttgtaaaag tgataaatta tggagaacca attccagtgt tagatttacc aaatatattt
     2581 gatagatttt atagagttga aaaatcgaga aatagaaatg ttggaggttc tggattgggt
     2641 cttgcgatta caaaaaatat tgtaaattta catgatggag aaattactgt ttcaagcgat
     2701 aaatatcaaa ctgtgtttga ggtacaactg ccaataatac aatcagctta gttataaaat
     2761 aaaagtacga ttatgtgata taaatattat aacaataaag tgtttacaaa aataaaaaag
     2821 tgatatataa tttaagtcag tttaataaga tacttatcaa gaattagggg agagagttag
     2881 ctcagtgatc ctataccaac cttttatcag gtggtcctgc taacaacgat gagatgactt
     2941 gaaataatat actcttctta tcaataatag ataggaagag ttttttttag aattaatgta
     3001 acaaacttta aagtataatg gaggattaaa taatggaaaa agtagaaagt tttaagctag
     3061 accatacaaa agtaaaagct ccttttgtaa gaaagtgtag tgtacttgat ggagtaaagg
     3121 gagataaagt aactaaattt gacttgagat ttttacagcc aaatgtagaa agttttggaa
     3181 ctgctgctat gcacggttta gagcatcttt tagctactta tttaagagat acattagatg
     3241 gtgtaataga cttatctcca atgggatgta gaacaggatt ttatttaata ctatggggag
     3301 atgtagatgc caaaacagtt aagattggtt tagaagaagc attaaaaaaa gttcttgaat
     3361 cagataaaat gccagcagct acagccatag aatgtggaaa ctacagagac ttatctttat
     3421 ttggggcaaa ggaatatgca aaagatgttt tagataaagg attttcttta aatatatatg
     3481 gagaataatt taattggaag agaagatata agcataaatc aaagtgaagt tttaagatat
     3541 ttacaataca gaggtcaaga gatagataaa gaccttaaat atactattga tgaatgtgta
     3601 gctataacta aaaataaaat aaatcctaga tatttatcta ggatttatcc tattagaata
     3661 aataaagata atgttgtaga attagaggga acaaatttaa cattagagag taaagatgta
     3721 tatgaacttt taaaagaatg taatgagtgt gtaattatgg cagctactat agggatagat
     3781 atagaaaaag aaataagacg acactcttat tcaaatttaa caaaaggact aataattgat
     3841 tcatgtggaa caacagcaat agaagaagtg tgtgacattg ttcaaaataa tttagaaaag
     3901 gtttttttaa accaagataa atttataact atgcgatata gcccaggata tggagattta
     3961 ccaatagaaa aaaatataga tatattaaac gttttgaatg ctcaaaaaca aataggtcta
     4021 acaattactt ctaatggaat tatgataccg agaaagtcag tagtggctat tataggtatt
     4081 tctaatacaa gaataaataa atctaagaaa gaaaagtcgt gtgaaaattg taaaaactat
     4141 agaaactgta catatagaaa aggagcgaac tcttgtggaa ataagacaat atctgaaaaa
     4201 taatatatta atatttgatg gtgcaatggg aacaatgctt caacaaaaag ggttaaagtt
     4261 aggtgaaaat ccagaagtat ttggtcttca aaatccagat aaacttatag aaatacatac
     4321 agcatactta gaggcaggtt cgaatgtaat tcttacaaat acttttggat gtaatgaact
     4381 aaaattagat agtaagtata ctgtagaaga ggtgattgac aatgctgttt tggtggctag
     4441 aaaagctata gaaaatgtag ataatactaa accaagatat gtagctttag atattggtcc
     4501 tataggagaa atgctagagc ctatgggtac tttaagtttt gataatgcat atgagatatt
     4561 taaaagacaa gtactacaag gcgttaaatc tggagttgat gtaatagtaa tagagactat
     4621 gatggattta tatgaagcta aagtagctgt attagcagca aaagaaaact ctgatttacc
     4681 tattttctgt actatgacat ttgatgaagg tggaagaagt tttactggat gtatgccaga
     4741 atgtatggtg gctactatag aaggtctagg tgtagatgca ataggagtta attgttcatt
     4801 aggacctaaa caacttttac caatagttga aaaaatagca tcaagagcta cagtccctgt
     4861 tatggttcaa gcaaatgcag gtcttccaaa tatagttgat ggagaggcta tatatgatgt
     4921 tgatgcaaaa gaattttttg aaggggttaa aaagttcgtt gaagttggtg ctactataat
     4981 aggtggatgt tgtggtacga atccaagttt tataaaagaa atatctgaga atataaattc
     5041 tgttacaaag ggatgtatcg agaaaattga taaatgtgta gtctgttcac catcaaaatt
     5101 tgtggaagta caatcgccta cagttgttgg agaaagattg aatcccacag gtagaaaatc
     5161 tctacaagaa gcattaaaaa atgaaaatgt tgattatgct ataaatttag gcttagaaca
     5221 agtaaatgct ggagcacaaa tacttggagt aaatgttggt cttccagaga ttgatgaaaa
     5281 aaaactaatg ccaaaattaa taagagagat tcaagcagta gtagatacac ctttacaagt
     5341 tgattcttcc aatgtagagg ccttagagca aggacttaga tattataatg gtagaacaat
     5401 agtaaactct gtaaatggca aagaggagtc tttagaaagt atattaccaa tagttaaaaa
     5461 gtatggttca tgtgtggttg gacttacact tgatgaaaag gggataccta aaaaggctga
     5521 agaaagattt gaaatagcaa aaaggatagt caatagagca gtttcttgtg ggataaaacc
     5581 aaaagatata tttatagact gtttatcatt aactgtttca gcacaacaag aagaagcaat
     5641 agaaactata aaagctataa caatggtaaa gacactagga gtaaaaacta tattaggtgt
     5701 ttcaaatatt tcatttggtc ttccaaatag gaaagcactt aatgcttctt ttctaacatt
     5761 agctttaggg gcagggcttg atttagcaat tataaatcca aatgaatata gtatgatgga
     5821 agctatcaat tcttttaaaa tattaaataa cacagataaa ggatgtataa actatataaa
     5881 tcaatatagt aatataaata attcaaaaaa atcggattca tctacaaaga ttgacaaaga
     5941 tttgccattg gatattttag ttgaaagagg attaaaagat gaagcaaaaa atgtaacact
     6001 gaatctttta aaagagaagg atgaaaatta catattagat gagattttaa ttcctgctct
     6061 tgataaagta ggaaaaagat atgatagcgg agatatattt ttaccacaat tgattcaatc
     6121 agcagaaacg gtaaaagttt cattaaatac aataaaggaa actttattaa gtaaaagtag
     6181 caataatgta tccaaaggaa aaataatagt tgctacagta aaaggtgata tccatgatat
     6241 tgggaaaaat atagtaaaaa taatgctaga aaattatgga tatgaggtta tagatttagg
     6301 taaagatgtc cctattgaag aagtagtgga agttgctaaa aagagaaata taaaattagt
     6361 aggattaagt gcacttatga ctacgactgt tcaaagtatg aaagatacaa ttcaggcact
     6421 gagagataat gagataaatg caaaggtatt tgtaggaggt gcagttttga cagaagaata
     6481 cgctgaagaa atgggcgctg attattattc aaaggatgca aagtctgctg ttgaaatagc
     6541 aaagttaaat ttttagcata taaattaaaa taaaggtttt actaaatagg atattttata
     6601 tataatataa atataagttt tagatactta tgtttaaaac aataggaatt aatctataat
     6661 aatgagatta attaatataa aaaatattta tcatcaacat taaaaagggc tgggaagtct
     6721 ggtataactt ccattagccc tttttagtta aaatcatatg ttaaaaagcc caattaccat
     6781 ttttaaaaat ctgtatttgt tttccatcat aagtctcacc aataatatcc atatcagcag
     6841 aaccaatcat aaaatctaca tgtgttaaac tgtcatttac accatgttca tctaattctt
     6901 catcttttaa attgtttcca ccttccagac aagtcttata agcagaacca attgcaaagt
     6961 ggcaagaagc attttcatca tatagagtat tataaaaaat tatatccgta ttggatattg
     7021 gtgagttgta tggaacaaga gcaacttctc ctaaataatg agagccttca tccgtttcaa
     7081 taagtttttc tagtgtatct aatccctttt cagcagagaa atctattatt ttaccatctt
     7141 taaatgttaa gaagaaattg tcaattatgt ttccaccata aacaagaggt tttgtactat
     7201 aaacagtccc atttacttta aacttatgtg gcattccaaa aatctcttca gttggaatat
     7261 tcggattaaa acttattcca ttagggtctt tggatgctcc acttagccaa acatgaccct
     7321 ttggcaagtc tagagtcaaa tcagtttttg aggatttata ttttaaagtt ttaaagttat
     7381 ttttatttag aaagtccatt ttagatttaa gattttcatt atgttcttcc catgctttta
     7441 tagggtcctg accatctact cttgaacatt taaatatagc atcccacagt ttttctattg
     7501 ctttatcttc tgataaattt ggaaacactt tttttgccca atcttttgtt ggcacagaga
     7561 ctatactcca tttacattta tcagttagag tgtaagaacg ccattctttt aaagcttttc
     7621 cagatgcctt ctgaaaattt gcaactcttt cagggtcaac atcttttaat aaatctggat
     7681 tttgggcata gactgataaa aatgccccac cttcttttgc tatatctaca tattgttctg
     7741 ctgtccattt ggggaactca tgaaatgctt catctggagc atacaagtat ttaataagag
     7801 tactttcttc atcagaccac tctatataaa catttttagc accacattta tatgcttctt
     7861 taagtgcatt tcttgcaaaa tcagcacatt ctattggtga ctttataagt agaatttgac
     7921 ctggctgtat gttaactcca acttttattg ctaattcagc gtatttttct aaatttctat
     7981 ttaaattcat atttatcctc ctatattttt aatattgaat aacttataaa gtattataac
     8041 attacttatg tttttaatat tgaacaactt ataaaatatt ataactttac ttagattttg
     8101 ggtcatattc aaaatattta tactttatag attgagaaat aaaaaaaata ttttaattaa
     8161 aacagattat gaggataaca aacttattta agcagtatgt tttttgttga taataatatt
     8221 aaagtagaaa taaaaatact tttttagtga aaagctatac aagaataccg aaaaaaactt
     8281 ataatataat aatgagatag atatttataa atgaaaaaaa tt
//
LOCUS       DAEPOZ010000164         8316 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.164, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000164 DAEPOZ010000000
VERSION     DAEPOZ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8316
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.164"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..2431
                     /gene="tcdB"
                     /locus_tag="KR440_002722"
                     /gene_synonym="toxB"
     CDS             <1..2431
                     /gene="tcdB"
                     /locus_tag="KR440_002722"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HBH0699757.1"
                     /translation="DENGVAEILKFMNKKGSTNTSDSLMSFLESMNIKSIFINSLQSN
                     TKLILDTNFIISGTTSIGQFEFICDKDNNIQPYFIKFNTLETKYTLYVGNRQNMIVEP
                     NYDLDDSGDISSTVINFSQKYLYGIDSCVNKVIISPNIYTDEINITPIYEANNTYPEV
                     IVLDTNYISEKINININDLSIRYVWSNDGSDFILMSTDEENKVSQVKIRFTNVFKGNT
                     ISDKISFNFSDKQDVSINKVISTFTPSYYVEGLLNYDLGLISLYNEKFYINNFGMMVS
                     GLVYINDSLYYFKPPIKNLITGFTTIGDDKYYFNPDNGGAASVGETIIDGKNYYFSQN
                     GVLQTGVFSTEDGFKYFAPADTLDENLEGEAIDFTGKLTIDENVYYFGDNYRAAIEWQ
                     TLDDEVYYFSTDTGRAFKGLNQIGDDKFYFNSDGIMQKGFVNINDKTFYFDDSGVMKS
                     GYTEIDGKYFYFAENGEMQIGVFNTADGFKYFAHHDEDLGNEEGEALSYSGILNFNNK
                     IYYFDDSFTAVVGWKDLEDGSKYYFDEDTAEAYIGISIINDGKYYFNDSGIMQIGFVT
                     INNEVFYFSDSGIVESGMQNIDDNYFYIDENGLVQIGVFDTSDGYKYFAPANTVNDNI
                     YGQAVEYSGLVRVGEDVYYFGETYTIETGWIYDMENESDKYYFDPETKKAYKGINVID
                     DIKYYFDENGIMRTGLITFEDNHYYFNEDGIMQYGYLNIEDKTFYFSEDGIMQIGVFN
                     TPDGFKYFAHQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYF
                     DPDTAQLVISE"
     gene            2553..3053
                     /gene="tcdE"
                     /locus_tag="KR440_002723"
     CDS             2553..3053
                     /gene="tcdE"
                     /locus_tag="KR440_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HBH0699758.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAVLLDAMTDEM
                     NAKDEK"
     gene            3382..3564
                     /locus_tag="KR440_002724"
     CDS             3382..3564
                     /locus_tag="KR440_002724"
                     /inference="COORDINATES: protein motif:HMM:NF022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699759.1"
                     /translation="MRDEGQNDRENKYQTLINDLIDSLKVIGKIYKEIKTLKKYKICL
                     QITIRQSPYLIEESIN"
     gene            3781..>8316
                     /gene="tcdA"
                     /locus_tag="KR440_002725"
                     /gene_synonym="toxA"
     CDS             3781..>8316
                     /gene="tcdA"
                     /locus_tag="KR440_002725"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021394011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HBH0699760.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKNSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     LLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTIPRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKDSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKEYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPYISSHIPSLSV
                     YSAIGIKTENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENNGTKLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNTKIKLDKDTRNFIMPTITTDEIRNKLSYSFDGA
                     GGTYSLLLSSYPISMNINLSKDDLWIFNIDNEVREISIENGTIKKGNLIEDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNGSTLEFNSKDFIAED"
BASE COUNT         3377 a          901 c         1286 g         2752 t
ORIGIN      
        1 agatgaaaat ggagtagctg aaatattgaa atttatgaat aaaaaaggta gtacaaatac
       61 ttcagattct ttaatgagct ttttagaaag tatgaatata aaaagtattt tcataaattc
      121 cttacaatct aatactaagc ttatattaga tactaatttt ataataagtg gtactacttc
      181 tattggtcaa tttgagttta tttgtgataa agataataat atacaaccat atttcattaa
      241 gtttaataca ctagaaacta aatatactct atatgtaggt aatagacaaa atatgatagt
      301 agaaccaaat tatgatttag atgattctgg agatatatct tcaactgtca ttaatttttc
      361 tcagaaatac ctttatggaa tagacagttg tgttaataaa gttataattt cgccaaatat
      421 atatacagat gaaataaaca taacacctat atatgaagca aataatactt atccagaagt
      481 gattgtatta gatacaaatt atataagtga aaaaatcaat attaatatca atgatttatc
      541 tatacgatat gtatggagta atgatggaag tgattttatt cttatgtcaa ctgatgaaga
      601 gaacaaggta tcacaagtta aaataagatt tactaatgtt tttaaaggta atactatatc
      661 agataagata tcttttaatt ttagtgataa gcaagatgta tctataaata aagttatttc
      721 aacatttaca ccttcatatt atgtggaagg attacttaat tatgatttag gtctgatttc
      781 tttatacaat gagaaatttt atattaataa ctttggaatg atggtgtctg gattagtata
      841 tattaatgat tcattatatt atttcaagcc accaataaag aacttgataa ctggatttac
      901 aactataggt gatgataaat actactttaa tccagataat ggtggagctg cttcagtcgg
      961 agaaacaata attgatggca aaaactacta cttcagccaa aatggagtgt tacaaacagg
     1021 tgtatttagt acagaagatg gatttaaata ttttgctcca gcagatacac ttgatgaaaa
     1081 tctagagggg gaagcaattg attttactgg caaactaact attgatgaaa atgtttatta
     1141 ttttggagat aattatagag cagctataga atggcaaaca ttagatgatg aagtgtacta
     1201 ttttagtaca gatacaggta gagcttttaa agggctaaat caaataggtg atgataaatt
     1261 ctatttcaac tctgatggta ttatgcaaaa aggatttgtt aatataaatg ataagacatt
     1321 ctattttgat gattctggtg tgatgaagtc aggatatact gaaatagatg gaaaatattt
     1381 ttactttgct gagaatggag aaatgcaaat aggagtattt aatacagcag atggatttaa
     1441 atattttgct catcatgatg aagatttagg aaatgaagaa ggtgaagcac tttcatattc
     1501 tggtatactt aattttaaca ataagattta ttattttgat gattcattta cagcagtagt
     1561 tggatggaag gatttagaag atggttcaaa atattacttt gatgaagata cagcagaagc
     1621 atatataggt atctcaataa ttaatgatgg taaatattat tttaatgatt ctggaatcat
     1681 gcaaattgga tttgtcacaa taaataatga agtattttat ttctctgatt ctggaatagt
     1741 agaatctgga atgcaaaata tagatgataa ttatttctat atagacgaaa atggtctagt
     1801 tcaaattggt gtatttgaca cttcagatgg atataaatac tttgcaccag ctaatactgt
     1861 aaatgataat atctatggac aagcagttga atatagtggt ttagttagag ttggtgaaga
     1921 tgtatattat tttggagaaa catatacaat tgagactggt tggatatatg atatggaaaa
     1981 tgaaagtgat aaatattatt tcgatccaga aactaaaaaa gcatataaag gtattaatgt
     2041 aattgatgat ataaaatact attttgatga gaatggaata atgagaacag gtcttataac
     2101 atttgaagat aatcattact attttaatga agatggtatt atgcaatatg gttatctaaa
     2161 tatagaagat aagacgttct actttagtga agatggtatt atgcagattg gagtatttaa
     2221 tacaccagat ggatttaaat attttgcaca tcaaaatact ttagatgaga attttgaggg
     2281 agaatcaata aactatactg gttggttaga tttagatgaa aagagatatt attttacaga
     2341 tgaatatatt gcagcaactg gttcagttat tattgatggt gaggagtatt attttgatcc
     2401 tgatacagct caattagtga ttagtgaata gataaaaata cgttaaatat atcctcttat
     2461 acttaaatat ataaaaataa acaaaatgat acactacata aagtgttcta tctaatatga
     2521 agatttacca ataaaaaggt gaactatgat gaatgcacag tagttcacct ttttatattt
     2581 ctaatggtaa caaaatattt ttttatataa acctaggagg cgttatgaat atgacaatat
     2641 cttttttatc agagcatata tttataaagt tagtaatttt aactatatca tttgatacat
     2701 tattaggatg tttaagtgca ataaaaagtc gtaaatttaa ttctagtttt ggaatagatg
     2761 gaggaatcag aaaagtagca atgatagcat gtatattttt tttatcagta gttgacattc
     2821 ttacaaagtt taacttttta tttatgttac cacaagattg tatcaatttt ttaagactaa
     2881 aacatcttgg aatatctgaa tttttctcta ttttatttat tttatatgaa agtgtaagca
     2941 tattaaaaaa tatgtgctta tgtggattac cagtacctaa gagattaaag gaaaaaatag
     3001 cagttttact agatgcaatg acagatgaaa tgaatgctaa ggatgaaaag taagtaatgg
     3061 tagatataat aaaaatatta acaaataaaa agtgttatcc aaataagaat agctgaaagt
     3121 tatcataatt catgaaactg ataatgaaaa cgagggagca gatgccaaga gacacacaag
     3181 tattaaatac atataatttc gaagcaagtg ttcattacta tatggatgac aaggtagtat
     3241 atcaaacatt ggttcacaaa gatggtgcat ggtcagttgg taaaatctat taagctacat
     3301 tagttacaga tatcacaaac tataatagtt aaacatagaa atatgtgtaa attgtgatgg
     3361 aaattattca aaaacacaaa aatacgtgat gaaggacaaa atgatagaga aaataagtat
     3421 caaaccttaa taaatgattt aattgatagt ttaaaagtta taggaaaaat atataaagaa
     3481 ataaaaacat taaaaaaata taagatatgt ttacaaatta ctatcagaca atctccttat
     3541 ctaatagaag agtcaattaa ctaattgagt atctttaaat tgaaatgtta ggaagtgatt
     3601 taaatatgaa aacttaaatt ataaaaaatc aatattaatt tatctttaaa aaatagaaag
     3661 gagtgtataa gatttatttt caaagtttaa aaacaagaaa atcaatttaa atttcagaag
     3721 gaataaatgt ggttatagaa gtggatttat tatcaaaaat aatagtacta ggaggttttt
     3781 atgtctttaa tatctaaaga agagttaata aaactcgcat atagcattag accaagagaa
     3841 aatgagtata aaactatact aactaattta gacgaatata ataagttaac tacaaacaat
     3901 aatgaaaata aatatttaca attaaaaaaa ctaaatgaat caattgatgt ttttatgaat
     3961 aaatataaaa attcaagcag aaatagagca ctctctaatc taaaaaaaga tatattaaaa
     4021 gaagtaattc ttattaaaaa ttccaataca agtcctgtag aaaaaaattt acattttgta
     4081 tggataggtg gagaagtcag tgatattgct cttgaataca taaaacaatg ggctgatatt
     4141 aatgcagaat ataatattaa actgtggtat gatagtgaag cattcttagt caatacacta
     4201 aaaaaggcta tagttgaatc ttctaccact gaagcattac agctactaga ggaagagatt
     4261 caaaatcctc aatttgataa tatgaaattt tacaaaaaaa ggatggaatt tatatatgat
     4321 agacaaaaaa ggtttataaa ttattataaa tctcaaatca ataaacctac agtacctaca
     4381 atagatgata ttataaagtc tcatctagta tctgaatata atagagatga aactttatta
     4441 gaatcatata gaacaaattc tttgagaaaa ataaatagta atcatgggat agatatcagg
     4501 gctaatagtt tgtttacaga acaagagtta ttaaatattt atagtcagga gttgttaaat
     4561 cgtgggaatt tagctgcagc atctgacata gtaagattat tagccctaaa aaattttggc
     4621 ggagtatatt tagatgttga tatgcttcca ggtattcact ctgatttatt taaaacaata
     4681 cctagaccta gctctattgg actagaccgt tgggaaatga taaaattaga ggctattatg
     4741 aagtataaaa aatatataaa taattataca tcagaaaact ttgataaact tgatcaacaa
     4801 ttaaaagata attttaaact cattatagaa agtaaaagtg aaaaatctga gatattttct
     4861 aaattagaaa atttaaatgt atctgatctt gaaattaaaa tagctttcgc tttaggcagt
     4921 gttataaatc aagccttgat atcaaaacaa ggttcatatc ttactaacct agtaatagaa
     4981 caagtaaaaa atagatatca atttttaaac caacacctta acccagccat agagtctgac
     5041 aataacttca cagatactac taagattttt catgattcac tatttaattc agctaccgca
     5101 gaaaactcta tgtttttaac aaaaatagca ccatacttac aagtaggttt tatgccagaa
     5161 gctcgctcca caataagttt aagtggtcca ggagcttatg catcagctta ctatgatttc
     5221 ataaatttac aagaaaatac tatagaaaaa actttaaaag catcagattt aatagaattt
     5281 aaattcccag aaaataatct atctcaattg acagaacaag aaataaatag tctatggagc
     5341 tttgatcaag caagtgcaaa atatcaattt gagaaatatg taagagatta tactggtgga
     5401 tctctttctg aagacaatgg ggtagacttt aataaaaata ctgccctcga caaaaactat
     5461 ttattaaata ataaaattcc atcaaacaat gtagaagaag ctggaagtaa aaattatgtt
     5521 cattatatca tacagttaca aggagatgat ataagttatg aagcaacatg caatttattt
     5581 tctaaaaatc ctaaaaatag tattattata caacgaaata tgaatgaaag tgcaaaaagt
     5641 tactttttaa gtgatgatgg agaatctatt ttagaattaa ataaatatag gatacctgaa
     5701 agattaaaaa ataaggaaaa agtaaaagta acctttattg gacatggtaa agatgaattc
     5761 aacacaagcg aatttgctag attaagtgta gattcacttt ccaatgagat aagttcattt
     5821 ttagatacca taaaattaga tatatcacct aaaaatgtag aagtaaactt gcttggatgt
     5881 aatatgttta gttatgattt taatgttgaa gaaacttatc ctggtaagtt actattaagt
     5941 attatggaca aaattacttc cactttacct gatgtaaata aagattctat tactatagga
     6001 gcaaatcaat atgaagtaag aattaatagt gagggaagaa aagaacttct agctcactca
     6061 ggtaaatgga taaataaaga ggaagctatt atgagcgatt tatctagtaa agaatacatt
     6121 ttttttgatt ccatagataa taagctaaaa gcaaagtcca agaatattcc aggtttagcg
     6181 tcaatatcag aagatataaa aacattatta cttgatgcaa gtgttagtcc tgatacaaaa
     6241 tttattttaa ataatcttaa gcttaatatt gaatcttcta ttggtgatta catttattat
     6301 gaaaaattag aacctgttaa aaatataatc cacaattcta tagatgattt aatagatgag
     6361 ttcaatctac ttgaaaatgt atctgatgaa ttatatgaat taaaaaaatt aaataatcta
     6421 gatgagaagt atttaatatc ttttgaagat atctcaaaaa ataattcaac ttattctgta
     6481 agatttatta acaaaagtaa tggtgaatca gtttatgtag agacagaaaa agaaattttt
     6541 tcaaaatata gcgaacatat tacaaaagaa ataagtacta taaagaatag tataattaca
     6601 gatgttaatg gtaatttatt ggataatata cagttagatc atacttctca agttaataca
     6661 ttaaacgcag cattctttat tcaatcatta atagattata gtagcaataa agatgtactg
     6721 aatgatttaa gtacctcagt taaggttcaa ctttatgctc aactatttag tacaggttta
     6781 aatactatat atgactctat ccaattagta aatttaatat caaatgcagt aaatgatact
     6841 ataaatgtac tacctacaat aacagagggg atacctattg tatctactat attagacgga
     6901 ataaacttag gtgcagcaat taaggaatta ctagacgaac atgacccatt actaaaaaaa
     6961 gaactagaag ctaaggtggg tgttttagca ataaatatgt cattatctat agctgcaacg
     7021 gtagcttcaa ttgttggaat aggtgctgaa gttactattt tcttattacc tatagctggt
     7081 atatctgcgg gaataccttc attagttaat aatgaattaa tattgcatga taaggcaact
     7141 tcagtggtaa actattttaa tcatttgtct gaatctaaag aatatggccc tcttaaaaca
     7201 gaagatgata aaattttagt tcctattgat gatttagtaa tatcagaaat agattttaat
     7261 aataattcga taaaactagg aacatgtaat atattagcaa tggagggggg atcaggacac
     7321 acagtgactg gtaatataga tcactttttc tcatctccat atataagctc tcatattcct
     7381 tcattatcag tttattctgc aataggtata aaaacagaaa atctagattt ttcaaaaaaa
     7441 ataatgatgt taccaaatgc tccttcaaga gtgttttggt gggaaactgg agcagttcca
     7501 ggtttaagat cattggaaaa taatgggact aaattgcttg attcaataag agatttatac
     7561 ccaggcaaat tttactggag attctatgcc tttttcgatt atgcaataac tacattaaaa
     7621 ccagtgtatg aagacactaa tactaaaatt aaactagata aagatactag aaactttata
     7681 atgccaacta taactactga cgaaattaga aacaaattat cttattcatt tgatggagca
     7741 ggaggaactt actctttatt attatcttca tatccaatat caatgaatat aaatttatct
     7801 aaagatgatt tatggatatt taatattgat aatgaagtaa gagaaatatc tatagaaaat
     7861 ggtactatta aaaaaggaaa tttaatagaa gatgttttaa gtaaaattga tataaataaa
     7921 aataaactta ttataggcaa tcaaacaata gatttttcag gtgatataga taacaaagat
     7981 agatatatat tcttgacttg tgagttagat gataaaatta gtttaataat agaaataaat
     8041 cttgttgcaa aatcttatag tttgttattg tctggggata aaaattattt gatatccaat
     8101 ttatctaata ctattgagaa aatcaatact ttaggcctag atagtaaaaa tatagcttac
     8161 aattacactg atgaatctaa taataaatat tttggagcta tatctaaaac aagtcaaaaa
     8221 agcataatac attataaaaa agacagtaaa aatatattag aattttataa tggcagtaca
     8281 ttagaattta acagtaaaga ctttattgct gaagat
//
LOCUS       DAEPOZ010000165         8272 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.165, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000165 DAEPOZ010000000
VERSION     DAEPOZ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8272
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.165"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..506)
                     /locus_tag="KR440_002726"
     CDS             complement(<1..506)
                     /locus_tag="KR440_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HBH0699761.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPIEVFSVCKNLDKNDLEIANEIELVEDRLVNFVEDSFIICHDLSLKKK
                     FFEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLV"
     gene            727..1551
                     /gene="pdaA"
                     /locus_tag="KR440_002727"
     CDS             727..1551
                     /gene="pdaA"
                     /locus_tag="KR440_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBH0699762.1"
                     /translation="MKKDSLKKYIMIGAFALILFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            1740..2504
                     /locus_tag="KR440_002728"
     CDS             1740..2504
                     /locus_tag="KR440_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0699763.1"
                     /translation="MTTICVMIDHIFLDTEYRQPTIHKHLAKHIIISQSGNLSVTFDK
                     GESLQCKGIIIDSNVSHTVSSKDNKMLVFLIDDTSILAQNISQHQLLNHSFFVIDNLI
                     VERIQNIYTACQVKGLSQSYIDFSKQAFRLLNLNYHISSITDSRIQWALNHILKSTHI
                     HNEIFFEICAEVKLSKSRFSHLFKEQMGVSLNSYLALSKLRKAYELLLKSGDITSAAM
                     DAGFSSPSHFSASSKKYLGITASELIGKCKIYFIRQ"
     gene            2607..3785
                     /locus_tag="KR440_002729"
     CDS             2607..3785
                     /locus_tag="KR440_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBH0699764.1"
                     /translation="MKTNKISFNKGLYYLNTNANFNFQLNRIIMWDGGRLEDIQKVSS
                     KIVDSESWKTELMYLAQEAEQDGRTENAIAYYRMSEFFMYDGDPDKISVYKKATTLFY
                     NYYSEYFSNGTVQIFHVPYENITLPVMYAKAQGEVKDVILLHGGNDSYFEEFFFPMLY
                     LASQGFDIYLFEGPGQGGVMRLQGKHFTYQWERPVKTILDYFHLENITIVGASLGGFL
                     APRAAAFEKRIERVVAWSVFPNFLDIIVGSQPIKMQNLLRLLLKLKTKWLVNFIIKQK
                     MKSGDSMVVWGLKHGMYAYEASTPYEYFQKIKYYQIDSIAKNINQDILILGATQDHFI
                     DYRTVGKEIDLLNNVRSLTFRLMTEAEQASNHCNCGNSKLVLDTISNWIMMMKIHDRV
                     "
     gene            complement(4156..4941)
                     /locus_tag="KR440_002730"
     CDS             complement(4156..4941)
                     /locus_tag="KR440_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0699765.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEDGNITSIILKNKSTLIIYSFKNNFDIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKEHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            5406..5771
                     /locus_tag="KR440_002731"
     CDS             5406..5771
                     /locus_tag="KR440_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0699766.1"
                     /translation="MKIRAEYTCPLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            5849..6403
                     /locus_tag="KR440_002732"
     CDS             5849..6403
                     /locus_tag="KR440_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBH0699767.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIIIKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYHK"
     gene            6623..7297
                     /locus_tag="KR440_002733"
     CDS             6623..7297
                     /locus_tag="KR440_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBH0699768.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     regulatory      7652..7748
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            7802..7978
                     /locus_tag="KR440_002734"
     CDS             7802..7978
                     /locus_tag="KR440_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699769.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
BASE COUNT         3017 a         1194 c         1091 g         2970 t
ORIGIN      
        1 tttactaaaa ggcaatttac aatatttatt atatcaatag catctgataa agctctattt
       61 tccactttag aattacaatt tgttaaagta ttttttaagt attctaagct ataatcttta
      121 tgatatggtt ctaaaataac tgctaattca attaaatcaa taaatttgtt ttttaacttt
      181 ggcatatgat actcaaaaaa cttttttttc agacttaaat catgacatat aatgaaacta
      241 tcttctacaa aatttacaag tctatcttcc accagttcta tttcattagc tatttctaaa
      301 tcatttttat ctaaattttt acatactgag aatacttcta taggtacctc aaatttgttt
      361 tttattaatg attcataggt atatattttc cagtctttaa ccttgacagc tccaacttct
      421 aaaatttctg aatttaaaca atctaatcca ctaacttcta tatctaaaaa aacaacatca
      481 ttcaatacac ttattatatt atccattttt atatcccttc aagttaaacc agcctaactt
      541 atttacaatc atttctcttt atattttatc atttatatac actttattta agataattaa
      601 tatcaacttt atatcttaca gtaaacttct ttcatttatt atttgtatat gtgaatattt
      661 ttaactataa atattcaaac tatattataa gaaatatact ttaaatttta aataagaggt
      721 gaaattgtga aaaaagatag cctaaaaaaa tacattatga taggagcatt tgctctaata
      781 ctttttggaa ttgctagtat aaattttaaa tcattagata aaacaaaaac tcaaatttct
      841 agtccaacac tcgacacaca tgaatatgat tggtatttta accctaggga ggatggtaaa
      901 cagccatcac ctataaaaga agctgatttt tttaaaaaat acggagctta ttatgtagga
      961 aatcccaatg aaaaagttat atatctatca tttgatgctg gatatgaaag tggaaacact
     1021 ccaaaattat tagatacgct taaaaaacac aatgctaaag ctcaattttt cgtagttgaa
     1081 agttatataa aaagcaatcc agagttaata aagcgtatgg aaaaagaagg acatttagtt
     1141 tgtaatcatt caaaaagcca tccatcaatg gcaggtatta cagattttga aaaattcaaa
     1201 gaagaaatta caagtgtaga aaaagcatac aaagatgtta ctggaaaaga aatgcctaag
     1261 tattttagac ctccaatggg taaatttagc gaacaatctt taaaatatac acaagattta
     1321 ggatataaat caatattttg gagttttgct tatgtagatt ggtatgaaaa gaaacaacct
     1381 actcatgaat ttgccaaaaa caaaatttat tcaagaactc atcctggtgc aatagtttta
     1441 ctgcatccaa attcatcaac aaatactgag atattagatg aggttcttac acattgggaa
     1501 aaagaaggtt ataaacttaa aacacttgat tatttaaata ataaaaaata gtatcataaa
     1561 ttttaaaaaa ctctatcatg aatcatagta aaaatatagt tatttataaa tactttaatt
     1621 ctcgatagag ttttttattt ttcattgcct aaaataggat ttttaaatac ttgtaatcac
     1681 taagctttct tgttaaaaac tgtataatat gctaagctat gaacaaggag ttgacagcta
     1741 tgactaccat ttgtgtaatg attgaccata tatttttaga tacagagtat agacaaccaa
     1801 ctatccataa acatcttgca aaacatatta ttatatcaca gtctggtaac ttatctgtga
     1861 cgtttgataa gggcgaatca ttacaatgta aaggaattat tattgattcc aatgtatcgc
     1921 atacggtgtc aagtaaagat aataaaatgt tggttttctt gatagatgat acaagcatac
     1981 tcgcccaaaa tattagtcaa caccaattac taaatcactc ttttttcgtt atagataatc
     2041 ttatagttga acgaatacaa aatatttata cagcttgtca ggtaaaagga ttgtcccaaa
     2101 gctatattga tttttcaaaa caagctttcc gtctattgaa tttaaattat catatttcgt
     2161 ctattacaga cagtagaata caatgggcat tgaatcatat tctaaagtcc acacacattc
     2221 acaatgaaat cttttttgag atatgtgccg aagtaaaatt gtccaaaagc cggttttctc
     2281 atcttttcaa agaacaaatg ggagtatcac taaacagcta tttggcttta tcaaaacttc
     2341 gcaaggctta tgagttattg ttaaaaagtg gagatattac ctcagctgct atggacgcag
     2401 ggtttagttc accgtctcat ttctcagctt ccagcaaaaa atatcttggc attactgcaa
     2461 gtgagcttat aggaaaatgt aaaatatatt ttatccgcca atagcgtcta tttgaaagtc
     2521 agtacttcta atatttgtta caataaatgc atagcattaa agattactct ttattgctat
     2581 gcattatctt tttaggagtg atgacattga aaacaaataa aatttctttt aataaaggtc
     2641 tatattacct taatacaaat gccaatttta attttcagtt aaacaggatt attatgtggg
     2701 atggggggcg cttggaagat atacaaaaag tttcctctaa aattgtcgat agcgaaagtt
     2761 ggaaaacaga acttatgtat ctggcacaag aagctgaaca agatggacgc acagaaaatg
     2821 caattgctta ctatcgtatg agtgaatttt ttatgtatga tggtgaccct gataaaatat
     2881 cagtctacaa aaaagcaact actctattct acaactacta ctctgaatat ttctcaaatg
     2941 gaactgtaca aatttttcat gtaccatatg aaaatatcac cctacctgta atgtatgcaa
     3001 aagcgcaagg cgaggtaaaa gatgttatat tgctccacgg tggaaatgat tcttactttg
     3061 aagaattctt tttcccaatg ctttatcttg cgtcacaggg atttgatatt tatctttttg
     3121 agggacctgg tcaaggtgga gtgatgcgtt tgcagggaaa acactttact tatcaatggg
     3181 aacgtcccgt taagacaatt ttagattatt ttcatttaga gaatataact attgtaggtg
     3241 cttccttggg aggttttctt gctccacgag cagcagcctt cgaaaaacga attgaacgcg
     3301 tggtagcttg gtctgtattc cccaatttct tagacataat agttggttca caaccaataa
     3361 aaatgcaaaa tctacttaga ctactgctaa aactcaaaac aaaatggcta gtcaatttta
     3421 taataaagca aaaaatgaaa agtggcgata gtatggtggt atggggttta aaacatggaa
     3481 tgtacgcata tgaagcgtct acaccctatg aatattttca gaaaatcaag tattatcaaa
     3541 tagattctat tgcaaaaaat ataaaccaag acattttgat tttaggtgca acacaagatc
     3601 attttattga ttatagaact gttggtaaag agattgattt actcaacaat gtccgatccc
     3661 ttacatttag gcttatgaca gaagcagaac aagctagtaa ccactgtaac tgtggaaata
     3721 gcaaactggt tttagataca atttcaaatt ggattatgat gatgaaaata catgatagag
     3781 tctaaatatt gatttttaaa ttatacaaaa tccatctgct agcatatcct tattgctttg
     3841 ttcaaataat cacttgttaa aaaattggtt cccacttacg aaatactacg tttatagaaa
     3901 tgaactatat catgtaagca cacaatatat atgataataa gacccaatta ctcaatgtaa
     3961 ctgggtcttt cttatgtcat agattttata ggactcatga aatgtaaaag agtatttcac
     4021 cgatttccaa agggaacttt acttagataa ggagaccata aatgctaaca aacccatcta
     4081 ctagacacct ttcaagtatt tgagcgatta tacaatacac gttaagaatt acacgaaaat
     4141 ttattatttt tatagctatt taaaaagaat tgtccattca tttatctctt tgtatcctat
     4201 attatgatat attttacctg ctacatcatt attataaaaa agacatggaa cttttccttc
     4261 caacaataat tctttactca aattgtaaac catataactt gcaagtcctt ttcccctata
     4321 tcttttatct gtacttacag agaccaccat tgccaaaaaa ctagtttcca taccagttga
     4381 cacagtgcta atcatagtat catcatttct aataaaatat gctcttacat ttccctcttt
     4441 taaatgttct ttcctttctt ctatgtaatt tttacttact ttatagtctg acctatttaa
     4501 aagttttcct atttcatcaa tatctttttc ctgagcattt tctattttat actccttcat
     4561 gttggaaaaa tctatttctt ttatttcttt taatacacaa aatttattat ctagtttttc
     4621 ataaaaatct ttgtatttac ttattagtct atctattaca catttttttc cacttatgct
     4681 ttctacatct aaatctttta tatgattctt catttctcct atatcaaaat tatttttaaa
     4741 actataaatt ataagtgttg acttattttt taatattata gaggttatat ttccatcttc
     4801 tacctgagac catatatctt gatattgaga gctaaatcca taattctcaa cgtatccaat
     4861 tatgaataaa tttaaacttt cttcatgtga tacatattgc caaaattctt tttctaaagt
     4921 attatctatt tttacaatca agcagatatc ctccttctat gttaataatg atatacctta
     4981 taatttatag tatcatttaa aatcattata ttctaccaaa tattctaaat ttttaaatat
     5041 ctatgcaata tgttcgtatt tatttttata ttctgttact gcttcactcc aagcttcttc
     5101 tactgaaata cctctcttat ttgcaatttt ttgtgctaat tctattattt tcatagcttt
     5161 tcttgatata ttcatatata ttcctcctct aaattatcat aagttatttt aaagttaaat
     5221 ttctatagtc atgttatata tttatttact tatatcttac taattattta tgtactaaat
     5281 atgaactaca atagaagaaa tttagaactt aataaaacat aaaatttata tacagttcat
     5341 atatatttgt catatcacaa gacaagtgat ataatttatt atcattttac tgggggtgat
     5401 atgctatgaa aataagagct gaatatacat gtcctcttga aattgtacat gacataatca
     5461 aaggaaaatg gaaaactatc cttatatttg aattaagaca atctaataaa acattttctg
     5521 aattgcaaca taatatttat ggaataaatc aaaaaatgtt gattcaacaa ttaaaagagt
     5581 tacgtgattt tgggattatt gataaaataa gttctgctgg atatccatta catgttgaat
     5641 attttcttac taatcgtggt aaaaaaatgt taaaagcagt ggaaataatg caagaaatag
     5701 gaatcgaata tatgcttgaa catggtcaac aagaaattct agataataaa ggaatttgct
     5761 acaataagta atcctataca taccaaaaag taagtaattt acatttttta caatagtatt
     5821 ataataaaag taaatagaag gtataatcat gtctatagct ttaatattga ttgatattca
     5881 aaatgattat tttaaaaatg gaaaatgtga attatttcaa tcagaagaaa ctgctgaaaa
     5941 tgctaaaaaa atactactgt tttttagaaa aaacaattta ccagttattc atatacagca
     6001 tatatcaaca gataaaacag cttctttttt tcttcctaat actcatggtt cagaaataaa
     6061 taaaattgtt tttccactag ataatgaaga aattataatt aaacacacac cagatagttt
     6121 ctttgaaaca gatttgcaaa gtatccttga gaaaaaaaat attactaatc tagtaatctg
     6181 tggaatgatg agccatatgt gtattgatac aagtgtaaga acagcaaaga aacttcgata
     6241 cgacataact ctaataagcg atgcatgtac aacgaagaat ttatcttgga ataatataga
     6301 aattaatgca aatactgtac atcaagtatt catggcatca ctacagaaca gttttgcaga
     6361 tgtaaaaaac acagataaat ttttatctaa ctatcacaaa taaaaacata cactatgtta
     6421 aaagagcaag tttgatttat ttaagcttgc tctttaatca tttaaaatta gatgctttct
     6481 atcattttta tatacataat atacgtttat acattttata ttcatatata actattatat
     6541 aaattttaaa ctttactata ttcacataaa aaatgtagta ttatagtata tataatatta
     6601 tatagaaggg ggtgctagat aaatgaacca tcatatattg attcaatttg aatatatagc
     6661 aagaatcatt actgctggtt tctgtggggc tttaattgga tatgagagaa agaatagact
     6721 aaaagaagct ggaatacgca ctcattttat agtggcatta gcttcagcac ttataatcat
     6781 tgtttccaag tatggattta atgatatagt gggtactcca ggtattggtc tagacccagc
     6841 ccgcgttgca gcacaagttg tcagtggtat aggtttttta ggtgctggat taatatttat
     6901 tagaaatcaa tcagtaagtg gtcttactac agctgctggt atatgggcaa cagccggtac
     6961 aggtcttgcc attggctctg gattatactt agttggtatt gtttcaagta tcattattgt
     7021 tattgtacag ataacacttc atagagatag gaagtggatg aaactcccta cagcagagca
     7081 agtttctctt aaaatttcta acagcactga ttccatagaa tatattgaag gtaaatttaa
     7141 aaaatctaat attgaaatta taaacatgaa tattaaacgc cttgatgggg attggttaga
     7201 agtagttata tatactaagt tacctaaagg atataaaaaa acaaatttat taaaattatt
     7261 tagcgataat cctcttatag aaactttaga aatataatat aaaaattgaa gcttaaaaat
     7321 aataatatat ttaatcaagg tcaatatata aataattacg ggatgagtct atataaaatt
     7381 catcccttgt tttttgccat tcatatatat tttttcaaga tatttttgct catctattat
     7441 ctagccaagt aactcttgtg aactaaactt tttaatacaa aaacttcaat acaaaactaa
     7501 atataatttt tattaaatat tctacatgta tagtttgttt cttgagtgaa aaatagattt
     7561 catattgtta agatactgtt aaatatttca attttctgac aagtatgttg aacaataact
     7621 tatcctagtg tattatttta gttgtagaat aaaactaaat cggcaaaact agagaaattt
     7681 agtgacgcaa agctataggg actaacgctt ataaaataaa ctatgagtta tgtcagccag
     7741 ttgccaaaaa gatattgttc tttttgtttt atgaaagttt tttaggggga tagtaatata
     7801 tatgtttaga gatgaaatgg ataaatgtac acacatgtta actgcttata ttagtagttt
     7861 atatgattat tgtgatttta tagatacaca gctagatgat tttatactag agtacggaga
     7921 aaatgtagta gaatcttgtt tacatcaagt gatggtattg gtaagtaagt ataattaaat
     7981 agaaattgtt ttctagagaa ccctttttag ttttatctac atttaaaaat gttattatat
     8041 taaaaaacta atctccccta tatgcaaagt tttcttccat ataacaattt cttcccattt
     8101 atactttttc acttgtatct ttaataaaat tcaacttcaa atattaatat acttcaaaaa
     8161 ttattctatt tcttatcttt acaccaattc ctatcagtat tatcctttat ataggttcta
     8221 actatttcgc cacaatctaa acataaatct gaatatatag tagattgtac tg
//
LOCUS       DAEPOZ010000166         8267 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.166, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000166 DAEPOZ010000000
VERSION     DAEPOZ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8267
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.166"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            211..1155
                     /locus_tag="KR440_002735"
     CDS             211..1155
                     /locus_tag="KR440_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBH0699770.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLI
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
     gene            1378..1923
                     /locus_tag="KR440_002736"
     CDS             1378..1923
                     /locus_tag="KR440_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBH0699771.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            2014..3162
                     /locus_tag="KR440_002737"
     CDS             2014..3162
                     /locus_tag="KR440_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBH0699772.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQNSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSKKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            3215..4480
                     /locus_tag="KR440_002738"
     CDS             3215..4480
                     /locus_tag="KR440_002738"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBH0699773.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(4766..5998)
                     /locus_tag="KR440_002739"
     CDS             complement(4766..5998)
                     /locus_tag="KR440_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0699774.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFAATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(6001..6675)
                     /locus_tag="KR440_002740"
     CDS             complement(6001..6675)
                     /locus_tag="KR440_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0699775.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLVN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            complement(6683..7912)
                     /locus_tag="KR440_002741"
     CDS             complement(6683..7912)
                     /locus_tag="KR440_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBH0699776.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            complement(8024..>8267)
                     /locus_tag="KR440_002742"
     CDS             complement(8024..>8267)
                     /locus_tag="KR440_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0699777.1"
                     /translation="RTKNYLQLRVEDTGCGIKKEDLNRIFNKGFTGNNGRNNSKSTGL
                     GLYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL"
BASE COUNT         2958 a         1092 c         1363 g         2854 t
ORIGIN      
        1 tttagtatag gtaaaaaaat ataaaaaata taaaaattta ttaaaaaggt gtttacaaaa
       61 aatgattaat atattactgt taaatagtgg atataaattt gagctcaaaa tgtatgtaaa
      121 tttagattta aaatactgaa aaaatttact atattaattt atagtaatgc ttatagaaaa
      181 actctcttgc tgtttagggg gtttataatt ttgcaaaaaa ataaaaagga gggggcttta
      241 ttgaaaaaat actttggaga aataggacta atatttatag caattatatg gggaagtggc
      301 tttgtagcaa cccaatttgc attagacggt gggcttacac ctctacaaat cataacttta
      361 agatttttct tggcagctat aattatgaat ttactgtttt ttaaacagat tcgtgccaat
      421 atgggtaaaa aattattaaa agcaggaggg attttaggaa tatttttatt tctagcattc
      481 acagtacaaa ctattggact tatgtacact acaccatcta aaaatgcatt tataacagca
      541 gctaatgttg taatagttcc gttcatagga tttatacttt atagaagaaa acttgataaa
      601 ataggaataa taagtagttt ggtagctctt ataggaattg ggatactttc tttagaagct
      661 gatttctcta ttaattttgg agattttcta acacttatat gttcattcgg atttgcattt
      721 catatattct ttacaagtga gtttgcaaaa gataacaacc caatggcatt gacagctatt
      781 caatttacag tagcgttttt aatgtctgtt gtagttcaaa catttgcagg tcaattaaag
      841 atggaagctg aattatctgg ttatatggga actatgtatc tagctgtatt tagtactaca
      901 attggatttt tatttcagac tatatgtcaa aaaagagttg atggaactag aacagctata
      961 atattatcca cagaggctgt atttggtaca atattttcta taataattct aaaggaactt
     1021 attacagcaa aattgattat tggtagtatc ttgatatttg ttgcaatcat aactgcagaa
     1081 actaaattat cttttttaaa gagcaaaaaa gtgaaactta aagattcaga ggagtcttca
     1141 ttagaaagta tttaatatga caactatttc ctaattaatc aaataagtta tgtattatgt
     1201 agaaatttga catcaaaact tggttgatac tggtgtttca aagtgataaa attaattagg
     1261 aaataaattt tatttttagt gtttaagcta taaagtctgg ggtatatata ttcttgtagc
     1321 aagttattaa acttaaaaat aaaaatttaa ttatataaaa ttgggaggaa attaattatg
     1381 aaaaaatttg tttgtacagt atgtggatat atacatgaag gagatgctgc accagcacaa
     1441 tgtccagtat gtaaagttgg agctgataaa tttgaagaaa tgaaaggcga aatggtttgg
     1501 gctgatgaac atagaatagg agtagctcaa ggtgtagatg cagaaataat cgaaggatta
     1561 agagctaact ttactggtga gtgtacagaa gtaggaatgt atttagcaat gagtagacaa
     1621 gctgatagag aaggttatcc agaagtagct gaagcatata agagaatagc ttttgaagag
     1681 gctgaacatg ctgctaaatt tgcagaactt cttggagaag ttgtagttgc agatacaaaa
     1741 gaaaacttaa gagttagagt tgatgctgag tatggtgcaa ctgatggaaa attaaaatta
     1801 gctaagagag ctaaagaatt aggattagat gctatacatg atacagtaca tgaaatgtgt
     1861 aaagacgaag ctagacatgg taaagcattc ttaggattat taaacagaca ttttggaaaa
     1921 taataattaa aacatagatt aaaaggagaa actttggttt ctcctttttt aaattataca
     1981 aataaactgt tgtgatagaa ggatggagat aatatgaagt cggtatctgt taggagttgt
     2041 aaaacttaca attatgaaga agtaaagtta tctattgaaa aaaatttaga ggatattggt
     2101 ggtatagata agtttgttaa aaaaggaagc aaagtactta taaaaccaaa tttattaatg
     2161 aaaaaaaagc ctgaagaagc tacaactaca catcctatgg ttgtaaaagt tgtatgtgaa
     2221 aaacttttag agttaaattg tgatattaca atagcagata gccctggtgg tccatataat
     2281 caaaattctc ttaagagcat ttataaggca tctggaattg aagaagtagc aaatgaactg
     2341 ggcattaagt taaattatga tgtttcagat gttaagatac atagtaaaaa agcacatgtt
     2401 ctaaaatata tggatattat taaaccaata gtagattcag attatataat aaatctttgt
     2461 aaattaaaga ctcatataat ggcaaccttt actggtggta caaaaaactt atatggatgc
     2521 atagcaggat tgaaaaaagc agagatacat tatagatttc ctacagaaga gttgttttgc
     2581 gaaaatgtac ttttagatat ttgtgattat ataaaaccaa cattgacaat tatggatggt
     2641 atagtaggta tggaaggtga tggaccttca gctggtacta ctcgagaaat aggagtttta
     2701 ttagcatcac aaaatccata tgctatagat gttgttgcat gtaagattat aagtttactt
     2761 ccaaatagag ttgctacaat tagaggtgct attaagagag gctatataca agaagatttt
     2821 tcagatattg aaattttagg tgaagatata aaagatttaa ttataaaaaa ttacaagatt
     2881 ccaaatgtaa gtaaggattt aagattgttt tccgtaaagc taccaaaatt tttaaatgaa
     2941 cctatttcaa agcttataac accaaagcct gtggtaagat ttcaagattg cataaaatgt
     3001 ggaaaatgca aggaagcttg tcctgcatcc actatagaga tagggactaa aggtgctatt
     3061 atagacttaa gtaaatgtat aagatgttac tgttgccatg aactttgtcc taaaaaggct
     3121 attgatgtca aacgtaattt tgtatttaag tatgtaaaat aatgtataat attatatgag
     3181 tctatataaa ttaaaaatat aaaggagaaa taatatgatt ttaattaaaa atggtagagt
     3241 aatagaccct aaaagtcaaa gagatgagaa tattgactta attataaaag aaaataaaat
     3301 ttataaaata ggcaagtttg atgaaagtga tgaatatgaa aaaataatag atgctagtgg
     3361 taatgtagtt gctccaggtt tagtggatgt acatgtacac tttagagacc ctgggtttac
     3421 atataaagaa gatatagaaa gtggagctaa atctgctgct agaggtggat ttacaactgt
     3481 tatatgtatg gcaaatacta atcctattgt agataatgaa gatactttta attatgttaa
     3541 agaaaagtca aaaaatgcat gtataaatgt tttacaagca gcagctataa ctaaaggttt
     3601 tgaaggtaaa gagttagttg atatggaagc tcttaaaaaa gctggtgtac cagggtttac
     3661 tgatgatgga ttacctttaa tggatagcaa tctaattatg gaggcaatga ttaaggcaaa
     3721 agagttggat gtaccactta gttttcatga agaagaccct tctttagttg gaaatccagg
     3781 tgtaaatgca ggaaaggtag cttcagaact aggattaaaa ggtgcatcaa gtgtagcaga
     3841 agatgttatg gtagccagag attgtatgtt ggcactaaag actggtgcta aagttgatat
     3901 acaacatata agttcaggtg tatctgttga tatggttagg tttgcaaaat acttaggtgc
     3961 aaatgtagtt gcagaagctt caccacatca ctttacattg actgaggaag atgtattgga
     4021 gtttggaaca aatgcaaaaa tgaatccacc tctaagaagt aaatgggaca gagataagat
     4081 tatagaggga ttaaatgatg gtactattga aataatagca acggaccatg cacctcatag
     4141 taaagaagaa aaagatagag agtttataaa agctccaagt ggaattattg ggcttgaaac
     4201 ttctttagct cttggaataa caaatttggt gcataaaaat catcttagta tgatgcagtt
     4261 aatagaaaaa atgtctataa atcctgcaaa gttgtacaat ttaaatattg gatttataga
     4321 agaaggggct gtagcagaca ttgttatatt caatccagaa gaagaatgga cagttgaaag
     4381 ctttgtttct aaagctgata attcaccgtt taaaggtaaa tctttatatg gaaaggttaa
     4441 ttatacaata tgtaatgggg agattgtata taacgcctaa tttaagatta tttttttaag
     4501 gcggagtgta caatgattga aagtaaaaga ttgtatatca gcagaatgag attgattaat
     4561 caaaattgga agtattagtt tgaacaatta gaacataaca tcttggtaat ttagatatag
     4621 taaggaagag aatccttttt atgaaagaac gcactattat atttggaaag attaagttcc
     4681 taatctatca tatataatag tgcgttatat atttaagtat gttttatata aactaatcta
     4741 aaaatcgaaa atctttatac aatttctact tataaattgc ctttataggg tctagtttag
     4801 aagctttaaa ggctggaatc agaccaaata caactccagt caatatagta gctacaattg
     4861 cataaaataa gctatttaag ctaggtatag cttcaaaacc tatatattta gaaacatagt
     4921 tagtagcagc aaatccaact atagttccta aaattccacc acatactgtt ataaaaacag
     4981 cttcaactaa aaattgaaat aatatagacc taggttttgc tccaatagca cgtcttatac
     5041 caatttctct ttgtctttcc ataacagata catacattat attcattaca ccaataccac
     5101 caacaaacat tgcaactaca gttattatag atacatattt gtttacattg gaattaatgc
     5161 tttcaagata agctgtctgt tcagttgggt ctggtgtagt ataagaacca ttgataccag
     5221 ggtgcatctc atataattta gctataacat tgtttgcaac ttcatttaca ttatagcctt
     5281 tagaagaaac taagtctaac tggtaaattt catttgaata agaattttga ctcattaaag
     5341 tgtcaaaagc ctttttaggt actagggaag tagtaaattg catatcttgg taacctccaa
     5401 agaatcctcc agcttggttt tgctgagaat catctaatac ccctattatt tcaaatatag
     5461 ttccatttat gttgatacca tgacctaagg catcttctgg attttcaaaa atttcacttg
     5521 tactttgtaa tgtaagtaaa attacttttc ttttttcatc atctaaagaa aaatctctac
     5581 cacaaattaa atttatttta gaatcttttt ttactggtcc aacatcaaca tacgtagttt
     5641 ttttatcaaa agatgcttgt gaagagtaaa ctgaatcaag gttaaaccca tctcttgaag
     5701 gagctattct ttctactcct tcaacaaatg ataattcttc taaatctttt gcattaaagg
     5761 gttttaaaaa aatactcata tcatcagtaa gacctgtgtt atcggctgat tcaaacgaaa
     5821 tagtagtttt attagggttt acattgttta cagatttctt aatttctttt tgaaatccat
     5881 tacctataga actaacaagt attactgaag ttattcctat tataatccag agtaaagcta
     5941 caaatacacg aagcttgtga ccttttaaat tggccaaaga attttttaat aaactcatga
     6001 ttacacctcc gaagtaaaca caccatcttt aagtcgtata accttagttg catacttagt
     6061 taaatcttga tcatgtgtaa ccattattat agtcttacct tgtttgttga gccttgttaa
     6121 aagctccata atttctctgc tagtttcact atctaatgcc ccagtcggtt catcagcaaa
     6181 gattatctga gggtcattta ctaggcaacg tgcaatggct acacgctgtt gttgaccacc
     6241 agataattga agtggtttct gcttaagctt gtccaaaagc ccaacagaac ttaatttatc
     6301 tttaacaatt tcttctctat tagatttttt tagtgcttta ttgtaaatta gaggcaactc
     6361 tacattttga tatacattta gagtttctat taaattaaat tgttggaaaa caaatccaat
     6421 attaatattt ctaaaaacag aacgttcatt ttcactcaaa ttagttacat ctgttccatc
     6481 aaatatatat cttccttcat caaatacatc tagaaaacct aagatattaa gtaaagtagt
     6541 tttaccacta ccagacttac ccattatcat gacaaattct ccagattcaa tttctaaatt
     6601 taaggatttt aaaacatgta attcatcttt ccctactttg taatattttt gaatattttc
     6661 taatttaatt aacatatgta aattaattca catccatagt aacttcttct acttttttgt
     6721 ttggagtagt atctccatta ttagaaccag cagttacgcc atcgccttga actgggtctc
     6781 cttctttcat ttctttagta gggtgtttta ttattatatc gccttgttca agaccgcctc
     6841 taactacagc aaaatcatca ttttgacttg ctacatctac aatttgcttt ttcaatatac
     6901 catctaaatc cttaaataca tatgagtgtt tgttgtcttt aagtacacaa gatgctggta
     6961 ttttaaagct tgaattagct acttcaagtg atgcttgaag atggaatcca tttactaatc
     7021 cttcttgatt atcaaatgct atgtttatat cataataaga tagtgtattc tgctgagctc
     7081 ccatttcagt atttggagta gatggtttat cagatataaa acttattcta ccagtaagct
     7141 tttggtcagt tgaaaaaact agtatattga ccatttggtc aatcttcatt ttaggtaaat
     7201 cttgttcact tgcttgtcct ttcatataaa actctagacc ttgaatagtc ataaatgaaa
     7261 ctggttggtc tggatttcca gtttggtcat tcaagtatac tgtgccatca aaaggagcag
     7321 taactctgtc ataagctttt ttatccagag aagctatctg agagtttaaa gcagttatct
     7381 gtgcatttat agaagcaata gcatctttat tttcagcaat atcagaaagt gatatttttt
     7441 gttttttgta ttgactaagt tgtgatttta aactatctat ttctgctatt atagttggat
     7501 ttttagcagt aaaaagaagg tctccttgat ttactttttg accatttgta acattaacat
     7561 cacttaactc ataatctcca gatatattga agtccttagt ttgcttagga acaaccatcc
     7621 catttataaa tattttttca ttttcaggaa tagtatatgt ttcgataaaa ttttcattag
     7681 cttgtaattc agtgtttttc gaatcaacat aagcaagaac tcctagaatt aaaagtatga
     7741 ttactgctat tcctgcatat attttgaatc tcttattttt aaagggattg tttttaaaac
     7801 ctttattttt agacttgaag ccactacttc caaaactgct atttctaaag ttgttacttt
     7861 taaaactgtt atttctaaat cttttatttt taaatttttt tagaaaagac atgtcttcct
     7921 cctttttata aaaactactt aattatatac tgggatgtac atatagaatt actataagaa
     7981 gttgaatata agttaatgaa aataaactat caataataag atactataaa tcacatttga
     8041 atgtaatagt aaattttgta tattggttgt actctgaatc tacatcaatt ttatgaccaa
     8101 gcttatcaca aagttctttt actaaataaa gcccaagacc tgtagattta gaattgtttc
     8161 ttccattatt acctgtaaat cccttattga atattcgatt taaatcttcc ttttttatac
     8221 cacaaccagt atcttccact cttaattgta aataattttt ggttctt
//
LOCUS       DAEPOZ010000167         8115 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.167, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000167 DAEPOZ010000000
VERSION     DAEPOZ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8115
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.167"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            1..357
                     /locus_tag="KR440_002743"
                     /pseudo
     CDS             1..357
                     /locus_tag="KR440_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            475..1017
                     /locus_tag="KR440_002744"
                     /pseudo
     CDS             475..1017
                     /locus_tag="KR440_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            1162..1824
                     /locus_tag="KR440_002745"
     CDS             1162..1824
                     /locus_tag="KR440_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0699778.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCGNISLNKVEVKAYLDNVEVLLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            1824..3005
                     /locus_tag="KR440_002746"
     CDS             1824..3005
                     /locus_tag="KR440_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0699779.1"
                     /translation="MKTPRNINSTVVFIIFCLIITFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(3186..5846)
                     /locus_tag="KR440_002747"
     CDS             complement(3186..5846)
                     /locus_tag="KR440_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0699780.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAQKSKGESFKKISETTLTIVGKVDVSTSSVIPAYTALGYLDRTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKMS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYINSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKTVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNTRSRRQEFAMLRSVGLSPE
                     GIKKMLVLEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(5843..6526)
                     /locus_tag="KR440_002748"
     CDS             complement(5843..6526)
                     /locus_tag="KR440_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0699781.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            complement(6870..7808)
                     /gene="blaCDD"
                     /locus_tag="KR440_002749"
     CDS             complement(6870..7808)
                     /gene="blaCDD"
                     /locus_tag="KR440_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBH0699782.1"
                     /translation="MKRKNNFIWIVILLVVVAMGVYYVGRKHNNTKQKHDKNHINSEL
                     NKSKVENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETRRSPCS
                     TFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYYKKLID
                     KVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQTMAKI
                     FEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGMLEREGD
                     TYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            complement(7836..>8115)
                     /locus_tag="KR440_002750"
     CDS             complement(7836..>8115)
                     /locus_tag="KR440_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699783.1"
                     /translation="IQSDKNELEKIFSKEVIKKMKKLNRNLDVVKISGNRNLVYFFFG
                     AELQSIDGIVIFKDLKHVKPKLDFINRSIQAPRLEYITNNVYYFKGEL"
BASE COUNT         2814 a         1263 c         1061 g         2977 t
ORIGIN      
        1 tatctactaa attaataaaa gagaatgata ttatttgcat agaagattta caagtaaaga
       61 atatgattaa aaatcataaa ctatctcgct taatttctga tgtatcatgg tcagaattta
      121 ttcgtcaatt agaatacaag gctaattggt atggtagaca aattgtataa agtaggtaaa
      181 ttctttgcaa gttcacaaat atgcaataaa tgtgggtata aaaacgagga agttaagaac
      241 ttaaatataa gagaatggat ttgtcctagt tgtaatgaaa ctcacgatag agatataaat
      301 gcaagtataa atatattaaa agaaggatta agactaataa cgattcaaaa taaataactt
      361 atgtaagaac cgtaggaact acggggatgg cctgtggaga attagtaaga catattttag
      421 tatgcaaaat tctatgaagc aggaaccctg tgactttagt catgggaggt tcagaataca
      481 actatgaatg agttggcaaa agaagttgaa tatactaaac gcaccatata caagtacttt
      541 tcatgtaaag aagatttatt ctttgctgtt gtcttaagag gatataaaag actttgggat
      601 aatgttaaaa tagaaagtgc aaaaggcaaa acagggtttg aaaaaataaa actttcatac
      661 tttgcttttc ataaatttta ctctgtagaa ccttctctat tatgccttat gggaatgatt
      721 ggtattgtaa actctgaaaa atcggatact gatatgctgt ttaaagaaaa gtttttttct
      781 tttaataaat ttatgtttga tgaaatacaa ggaatgtttg aaattggaaa aaatgataga
      841 agtattaggc atgatattga aatacctact ctcatgtatt catcaatatt tacacttaca
      901 ggatttttta atttactatc tgtaacagga aagtcatacc tgaacaactt taatatagat
      961 gaggaaaaat ttattgaaac aactcttgct cttttaatag attcaattaa agcataaacc
     1021 taagtttata aattaaagtt tatatactta aatgataatt ttttctatct ctaacttcta
     1081 tctctaacat aaaaaatatt aaacataaaa ctaataactt agttaatgaa ataactaaat
     1141 tactgaaagg actaaaagta aatggctaaa atacttcttt tagaagatga tgatacaatt
     1201 acatttggaa taaaaacttc ccttgaaaag aaaaatcaca ctgtatgttg ttataatacg
     1261 attgctgaag ctaaaaaaaa ttttgataaa actattgatt taattctgct tgatttaaat
     1321 ctacctgatg gtattggata cgagttttgc caatatgtta aaagtataga agatactcca
     1381 gtgatatttt tgacagtccg tgatgatgaa aaagacatta tacgtggatt agatatgggt
     1441 gcagatgact atgtggttaa accatttctg ctctcagtac ttcaatccag aattaatgca
     1501 gttcttagaa gaactcattc aaatactaac aatactatta catgtggaaa tataagcttg
     1561 aataaagttg aagtcaaagc atatttagat aatgttgaag tacttttgac agcaggagaa
     1621 tataaacttc ttctaagtct tatggaaaat aaaaatagag ctatgacccg tacaaagtta
     1681 ctagaaatgc tctgggatgt agatggtaat ttcgtaaatg ataacactct tactgttaca
     1741 ataaagcgtc ttagagaaaa attaaaaaat ccaccttgta taaaaacatt aagaggcata
     1801 ggatatagaa tggaggacag ctagtgaaaa ctccaagaaa tatcaacagt acagttgttt
     1861 ttataatatt ctgcttaatt ataacttttt tattaagtgt aattctaaca actataactt
     1921 cttttaatga atacaatgta ctgtctaagg ctcttggtgc atcatatgag tacaatccta
     1981 agtctacaga aagtatacta aaatcactga aaaattctag tgataaggat tttaaaacag
     2041 gaaatgaaat tttatcaaaa catggatatt ctaaaagtac attttggagt aaaaactttt
     2101 tttattttct agtgatatcg tttggtataa tttcaacaat ggctacagtt gtatatataa
     2161 tcaaacatag aacaaataag caaaaactga gtagaataaa caatcttact caatacttag
     2221 aagaagtgaa tttaggaaaa gagtctgtac ttttacgatg tgaagatgaa ttttctcacc
     2281 ttgaagatga gatatataaa acagttgggg aacttcgttg ttcaaaagaa tttgctttaa
     2341 aagaaagaca aactctttca gataatcttg cagatattgc tcatcaactt aagactccaa
     2401 ttacttctat gtcacttatg gcacaattac tttcagaaaa ttgtccagat gaagaaatag
     2461 attatattaa cagacttaca aatcaaatct caagacttga gagactagtt tcatctttac
     2521 ttacattatc aaaattggat gcaagtactt taatatttga aagcaaactt atagatgtac
     2581 attctcttct tacttatgca attgagccta tagaaggttc tttaagagaa aaacatcaga
     2641 catttactat cgaggcttct ccttcggtag aatttgaaat cgatattaat tggactttag
     2701 aagctctttt aaatattctt aaaaattgta gtgagcatat agaagatggt ggatatatag
     2761 ttgcttatta tagtcaaaat cctctatatg ttgagattat catagaagat aatggtaaag
     2821 ggtttgctca ggaagaactt ccatatattt ttaatagatt ttatcgtggt aaaaatgcta
     2881 gtaaggacag tattggtata gggcttgctc tatcaaagtc tattatagaa cgtcaaaatg
     2941 gtactctaca tgcagaaaat tgccataatg gtggtgcaag attcataatt aaattttaca
     3001 cataaaatgc tggattatag gtaaaatacc tataatccag ctatatctat attctatgtt
     3061 atctattgtt ttagtaatat acttctattt acatttaagg ttttattaaa caaacaaatt
     3121 aactatccct atacttgtaa gtagtcttaa atcgaaacct ttaaataagt tcaaatagct
     3181 tttgtttata ttgtttcatt ttttatagat tctactatat tttcattctt caactgcttg
     3241 tatcctgtca tatatgatgc tattacaata aaaagtatca atatagtaaa acctattatt
     3301 gtagatatag gtgcaaatgg taaatactct ataaaatata tttcatttat acgtagaaaa
     3361 gcatacacta ttcctatttg cacaggaata ctaagtagta atggtgtaat tcctaagaac
     3421 aaaccttcca aaacaagcat cttttttatg ccttctggag ataatccaac agaacgcaac
     3481 atagcaaact cttgtcgtct acttctagta tttcctgaca ctgtagcaag tacatttgat
     3541 aatccaatca tagccaataa acctgtaata aagcctacta ttaaattcat ggtctttatt
     3601 ccactagcct tttgtttttc actttctaaa acatcctcaa tactataatc tcctgaacca
     3661 tagtctttat tataaatgga ctctaatttt tctcttactc taggaatgtc ctcacgatta
     3721 tttactttaa atactcctat cagcctattt gaacgtaacc tacttacttc atcataattt
     3781 ttagatattt cctgcaccat agacattggc actatttgca ttaatgtata ttttgaacta
     3841 gtatctactg ttttaggaag tgtgcttgta atagctccca ctttcatgtt gtaagtataa
     3901 tcccctttat cttcgtcata tactttttca tttaatttaa tgttatctcc tattgaaaga
     3961 tttaaataat ctatatatac tttatttcta cgtgtactac gattaatatc ttcctcctta
     4021 ttatatacaa ttgaggttgg attttttgta tcataaaata actttgggtc tacagaaatt
     4081 tgcctacaat attctgtaaa actcttatca tcaagcccaa ttatatttga aattatacga
     4141 aacttgccat cacgttctat aggagagtat tttttagatg aaactatact tttaaatcct
     4201 ccatttttcg caagttcttt tgatgcatct ttttcagtca gccaagtgga agcagacatt
     4261 ttttgataat acagtgaact ttttacacct tctgtattat ctaatttttc aatgaaagca
     4321 gactctacag gttcactatt ttccaaataa actgctatat ctctttggtc cttataatta
     4381 tcacttccat aaacagcttt tgctccttct tgatttgcaa ttatatgcaa aaaacatgta
     4441 agtaataaaa aagataatgt taaagatatt gttgcagtac gataagattt tttacgagca
     4501 cgtagtgcgt ttgatgcaag ttctccttct attccaaata tctttcctaa tgaatattta
     4561 tgcgactttt tgattttaac actatctcct tgtcgtattg cttctatagg agatatcttt
     4621 gatacttttc gtgctggtat aagtgcagaa atccagactg ttactattgt aagtaaaact
     4681 gctggcagaa aagcaggaat accataagta aataccactt ctggaacatc agtatgataa
     4741 ttaactgaat taatataaac tataagccta ttacacaata accatcctaa aaataaacct
     4801 attggcaatg gtataattgt aagcaaaaaa ccttcaaaca ctacagacat tttaatctgt
     4861 tttggtgatg caccaacact agataatatc cctaattgag taagtctagc atttgcagat
     4921 agtgaaaacg cgttgtaaat caccattaca aacacagcta ctgtaaaaat tgctattgta
     4981 agatacataa taggagttga aaattttgat atactgctca tagatgcttt ctgttctggg
     5041 gaaaagacta acatttttaa aagataatta ctattatatt taagattgta ttttccatat
     5101 tcattagtct tccaccccaa tgatttagca agttttggaa gttccttgta tgtgtctctt
     5161 atatttttaa atcgcaagta cacagttata ctatcattag gttttatact tgttctatca
     5221 agatatccaa gagctgtata tgcaggtatt actgatgaag tggaaacatc tacctttcct
     5281 actattgtca atgtagtctc tgaaattttt ttaaaacttt cccctttcga tttttgagct
     5341 atgggttcaa gaggctttcc atttaggatt ctatctccta ttggaagagt aattttatcc
     5401 ccaattttta cacttggatt attctcaaaa tattgttttg ataatgcaat ttcattagca
     5461 ttatttggtc gacgaccctc tattatagta tccttttctg gcatagaatt ccaatactct
     5521 gatgtagcat ttctataaat tatataatct cttttctcat cattaatttt gcctacatac
     5581 cattgtcctt ttaccattac atcatcaact gaagaaaaat tttcaattaa tggtaagtca
     5641 cttccataag tatagtcaaa caattcacca tgccagttac cttctttctc aattgaaagt
     5701 cttataaaat ctgtccacat ggtatacaac agtccacaca aagaagacat catggttgta
     5761 gtaagtaata tagctaacat tatagctata ctactacgtt tattgcgtcg tacaaagtct
     5821 aaggtgtatt cttgaagaat attcatttct tcaccacctt ttcagagaca atcactccat
     5881 cttccatagt tacaatacga tttgcctcta gtgctatttt ctcatcatga gtaataagaa
     5941 gtattgtctg attaaatcgt ttatttgata agtttaacaa gtctacaatt tcttctgaat
     6001 tttttctatc aagattacca gttggctcat ctgctaataa aatagctggt ctatagatta
     6061 aacttctagc tatagatacc ctttgttgtt gtccacctga aagttgactt ggaagatggt
     6121 ttaatctatc agataatccc aatatagata caatctcaga gaatctatcc tcatccacct
     6181 ttcttttatc aagtaacatg ggtaataata tattttttct aacatcaagg gtaggtataa
     6241 gattataaaa ttgataaata agacctactt ttctacgtct aaaaactgca agagcttctt
     6301 cctttaaact tgatatttct gtatcttcaa cataaatctt tccagatgta ggtctatcta
     6361 ctcccccaag cagatgtaac aatgtagatt tgccagaacc agatgctcct acaatggata
     6421 caaaatcacc tttttgaact gataaattta cattattaag ggctgtcact ctggtttgat
     6481 ttgaaccata tatttttgtg agattttcac attttaatat ttccatatac aatcatctcc
     6541 tttatggtta aattttagca acctaaaatg acattatagt gacatgaata caaatataaa
     6601 ttactattaa aaatattgtt tagtttttgc acattattta aaattataat ataaaacaaa
     6661 aactatgcaa tatattttat ttatactttt aatatattac atagtcttta aaaggtatta
     6721 actttaaatc tataggtatt caatttaata atcaatagca agtattttat gtactaaagg
     6781 agttttgctc tatatagact caatttagta aaattattta ataaatatta cagactatag
     6841 gtaaataata tttacaaata aaattaagtt tattccctta cactataata tttcttaatt
     6901 atattaattg caatttcctt tacctttggc cctgtaattt ctttattgga atcatctgat
     6961 ttaatagcaa aataataggt gtcaccttca cgctctagca taccaacaaa ccatgcatcc
     7021 acacgtttat ttttttcatc aaatcctgtt ccagtttttc catatacatt tatgttttta
     7081 tcatttacat ctattttcat aatatctctt aaaatattaa tatgctcttt tttaaagttt
     7141 gtatctccct caaatatttt tgccattgtt tgcacttgtt cttttggaga tatttgtaat
     7201 gaagattcta accaaaatcc atttaaatgt ccctttccat tttttaaatc tccttcccat
     7261 tcgctaatat cgcaattccc atactttaaa tcatctaaaa tgttttggac agatttagca
     7321 tcaactttat ctatcaattt tttgtagtac caaacacaag actccttaaa tgcctcttct
     7381 aaactaagat ttttattcca atttttattt ggatactctg taccatcata tcccataact
     7441 gattcttttg agtttattac ccctttttct aaaccaatta aagttgaaac tattttaaaa
     7501 gtagaacatg gtgaccttct tgtttcaatt aattctttgt tatatatatt atattcttta
     7561 tttttagtat tataaaaaat agctcctcca ctaatacctt caaaacagtc actatagtca
     7621 accatattaa ttttttcttt tttgttttta tcattgtttt caactttgga tttatttaat
     7681 tcagagttta tatgattctt atcatgtttt tgttttgtgt tgttatgctt tcttcctaca
     7741 taatatactc ccatagctac aacaactaga agaataacta tccaaataaa attatttttt
     7801 cttttcatac tttataccat ctcctctaca taagtttata gttcaccttt aaaataatat
     7861 acattatttg taatatactc caatctagga gcttgaatac ttctatttat aaaatctaac
     7921 tttggtttaa catgttttaa atctttaaaa atcacaatac catcaataga ttgtaattca
     7981 gctccaaaga aaaaatagac taaatttcta ttcccagaaa ttttaactac atctaaattt
     8041 ctgtttagtt ttttcatttt ttttattact tctttagaaa atatcttctc caattcattt
     8101 ttatcagatt gaata
//
LOCUS       DAEPOZ010000168         8089 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.168, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000168 DAEPOZ010000000
VERSION     DAEPOZ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8089
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.168"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            27..101
                     /locus_tag="KR440_002751"
     tRNA            27..101
                     /locus_tag="KR440_002751"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="KR440_002752"
     tRNA            116..200
                     /locus_tag="KR440_002752"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="KR440_002753"
     tRNA            210..293
                     /locus_tag="KR440_002753"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            317..391
                     /locus_tag="KR440_002754"
     tRNA            317..391
                     /locus_tag="KR440_002754"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:349..351,aa:Gly,seq:gcc)
     gene            416..492
                     /locus_tag="KR440_002755"
     tRNA            416..492
                     /locus_tag="KR440_002755"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:450..452,aa:Arg,seq:tct)
     gene            502..577
                     /locus_tag="KR440_002756"
     tRNA            502..577
                     /locus_tag="KR440_002756"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:535..537,aa:Gln,seq:ttg)
     gene            586..661
                     /locus_tag="KR440_002757"
     tRNA            586..661
                     /locus_tag="KR440_002757"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:619..621,aa:Lys,seq:ttt)
     gene            664..752
                     /locus_tag="KR440_002758"
     tRNA            664..752
                     /locus_tag="KR440_002758"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:698..700,aa:Ser,seq:tga)
     gene            756..831
                     /locus_tag="KR440_002759"
     tRNA            756..831
                     /locus_tag="KR440_002759"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:789..791,aa:Phe,seq:gaa)
     gene            838..911
                     /locus_tag="KR440_002760"
     tRNA            838..911
                     /locus_tag="KR440_002760"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Met,seq:cat)
     gene            926..1002
                     /locus_tag="KR440_002761"
     tRNA            926..1002
                     /locus_tag="KR440_002761"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:960..962,aa:Ile,seq:cat)
     gene            1020..1096
                     /locus_tag="KR440_002762"
     tRNA            1020..1096
                     /locus_tag="KR440_002762"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1054..1056,aa:His,seq:gtg)
     gene            1105..1180
                     /locus_tag="KR440_002763"
     tRNA            1105..1180
                     /locus_tag="KR440_002763"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1138..1140,aa:Lys,seq:ttt)
     gene            1188..1261
                     /locus_tag="KR440_002764"
     tRNA            1188..1261
                     /locus_tag="KR440_002764"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1220..1222,aa:Cys,seq:gca)
     gene            1269..1345
                     /locus_tag="KR440_002765"
     tRNA            1269..1345
                     /locus_tag="KR440_002765"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1303..1305,aa:Arg,seq:acg)
     gene            1358..1433
                     /locus_tag="KR440_002766"
     tRNA            1358..1433
                     /locus_tag="KR440_002766"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1391..1393,aa:Val,seq:tac)
     gene            1509..1922
                     /locus_tag="KR440_002767"
     CDS             1509..1922
                     /locus_tag="KR440_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699784.1"
                     /translation="MYIVIGNEIVDSEELKIIIDENSKFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLSSLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            2231..5662
                     /locus_tag="KR440_002768"
     CDS             2231..5662
                     /locus_tag="KR440_002768"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBH0699785.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5904..7832
                     /locus_tag="KR440_002769"
     CDS             5904..7832
                     /locus_tag="KR440_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBH0699786.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
BASE COUNT         3168 a         1024 c         1611 g         2286 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atgtaaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttat
      301 ttaatttaat aaacgagcgg gaatagttca gtggtagagc gcaaccttgc caaggttgaa
      361 gtcgcgagtt cgaatctcgt ttcccgctcc aaattttatg tttatgttgt aatgtgggtg
      421 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
      481 gtgcgctcac cacatatata ttggggatta gccaagtcgg taaggcacat gactttgact
      541 catgtatgcg taggttcgag tcctgcatcc ccagccaaac agtatggtct attagctcag
      601 tcggtagagc acctgacttt taatcagggt gtcccgcgtt cgagtcgcgg atagatcacc
      661 aatggagagg tgtccgagtg gtttaaggag ctggtcttga aaaccagtga cttcgaaagg
      721 ggccgtgggt tcgaatccca ccctctccgc caaatgccca gatagctcag tcggtagagc
      781 aggggactga aaatccccgt gtcggtggtt cgattccgcc tctgggcacc aaaatgtggc
      841 ggtatagctt agttggctag agcgttcggt tcatacccga aaggtcatag gttcgactcc
      901 tattaccgct actaaaattt aatgtgggcc attagctcag ttggttagag cgcccggctc
      961 ataaccggta ggtctggggt tcgagtccct gatggcccac cacattaaat taacttatgg
     1021 tgggtatagc tcagttggtt agagcgccag attgtggctc tggaggtcgt gagttcgact
     1081 ctcattatcc accccaactt atatggtcta ttagctcagt cggtagagca cctgactttt
     1141 aatcagggtg tcccgcgttc gagtcgcgga tagatcacca aaaaggcggc gacatagcca
     1201 agtggtaagg cagtggactg caactccttg atccccagtt cgaatctggg tgtcgcctcc
     1261 aataaaatgc gctcatagct caactggata gagtgtctga ctacgaatca gaaggttagg
     1321 ggttcgagtc cctttgggcg caccatatga atagcgaggg attatagctc agctgggaga
     1381 gcacctgcct tacaagcagg gggtcacagg ttcgagccct gttaatccca ccagtgcaaa
     1441 cctctagtaa aactactaga ggttttattt ttgattgaaa aagtttttat atttaggagg
     1501 taagaaattt gtatatagta ataggaaatg aaatagttga tagtgaagaa cttaaaataa
     1561 ttatagatga aaatagtaaa tttaaagttg aaaaagattt aagtaaaagc acaaaaagag
     1621 aagatgtaat agcatatcag ttaagtatag atttaaatta cctagattct cttataaatg
     1681 agcaatgtaa tttgtccagt ttatctgatg aagagaaatt tgatgaatat atgactttgt
     1741 ctgatgaact tgcactggat ttagaagagt taatgccaaa atatacaata ataaatgcta
     1801 gagcatataa attagacgag gtagatggaa ttgtaaaaat aatactagct gtagcgtatg
     1861 cagatttagg gcacttaaaa ctttctgatg tagtaaagag gctatctaga caagtagatt
     1921 aggatatgaa tatgaactat attaaaaaaa tgtataaaat cttacgtaaa gatgaaaata
     1981 attatgtaaa catagaaaaa aacagtataa aatcaagtca attgtagaaa ttttcacata
     2041 aattcatctg aaattctata aaaaatgcct taattttaat gaggtaaatc gagtttacac
     2101 agtctaaaaa ttacttatat ttataaattt atcttattaa ttaaggaact ttcttaaaca
     2161 tcttcaaaaa taaagtattt tagtctataa tctttaatat tacacaaaca ataaaggaga
     2221 agaatagagt atgcttaaaa agtttaataa aatactagtg gctaatagag gcgaaatagc
     2281 tataagaata tttagagcct gttcagaatt ggggataaaa agtgttggta tatacagtaa
     2341 ggaagataag tatggattgt ttagaacaaa agcagatgaa tcatatttga taggagaagg
     2401 taaagggcca atagatgcat atttagatat ggatggaata atagacctag caaagagaaa
     2461 aaaagttgat gctatacatc caggatatgg ttttctagcg gagaatgcag aatttgctcg
     2521 taaatgtgaa gagaatggaa ttacttttat aggaccatca tctaaggtaa tgaatatgat
     2581 gggggataag attaactcta aaaaaatcgc aaaagaagtt aatgttcaaa ctataccagg
     2641 agtagaaaaa gcaataagaa gtactgaaga agcaaaggaa gttgcaaaca aaataggata
     2701 tcctgttatg ataaaagcat ctaatggcgg tggcggtaga ggtatgagaa ttgtacatcg
     2761 cgaagaagac ttagaacttg aatatgaaac tgcttgtagt gaatcaagaa aagcttttgg
     2821 agaagatatt atatttatag aaaaatatat agctgatcca aaacatatag aagttcaaat
     2881 attaggtgat aattatggaa atatagttca cttatatgaa agagattgtt cagttcaaag
     2941 aagacatcaa aaaataatag aatatgcacc agctttttcg ttagatgata aagtaagaaa
     3001 agagatatgt gaagatgcag ttaagttatc aaaacatgtg ggttattcta atgcgggaac
     3061 attagaattt ttagtagatg caaatggtgg gcattatttt atagagatga acacaagagt
     3121 acaggttgaa catactgtaa cagagatggt tacaggaata gatatagttc aaagtcaaat
     3181 actaatagcc caaggttaca gcttagatag tgaagaaatt aatataaaat cacaagatga
     3241 tgtggaaata agaggttact ctatccaatg tagaataact acagaagacc ctaagaataa
     3301 attcatgcct gatacaggta aaattcaagt ttatagaact ggttcaggat ttggtataag
     3361 actagatgga ggaaatggat ttacaggggc aaacataagt cctcattatg atagtttatt
     3421 ggttaagaca atatcttggg atagaacatt ccaaggagca ataaataaaa ctataagatc
     3481 tataaaagaa cttagagtta gaggtgttaa aactaatgta ggattcctag ttaatgtttt
     3541 aaataatcct atattctcaa atggtaaatg tagtactaag tttatagatg aaaatccaga
     3601 tttatttgaa ataacagaaa gtaaagacag aggaacaaaa ttacttcaat ttataggaga
     3661 tgtaattgta aatgataatg cttgtaaaga aaaaccacta tttgatgcat tacatgaccc
     3721 aagaatggat aaggatggtt caaaatcaga aggaagtaaa atacttttcg ataagttagg
     3781 taaatctgca tatatagaaa agataaaaaa tgataagaaa ttactattaa cagatacaac
     3841 aatgagagat gcacatcaat cattattagc aacaagaata agaacatatg acttattaaa
     3901 agctgctaag ccaacagaaa aatatcaaaa ggatttattc tcacttgaaa tgtggggagg
     3961 agctacttat gatgttgcat atagattttt aaaagaatct ccttggcgta gattacaaaa
     4021 gctaagagaa gaaataccaa gcataatgtt ccaaatgttg cttagagcat caaatggagt
     4081 tggatataaa aactatccag ataatgtaat agaagaattt acaaaagaat ctgctagaca
     4141 aggtatagat gtatttagaa tatttgattc attaaactgg gttgaaaata tgaaaccatc
     4201 tattaataca gctttagaaa ctggaaaaat tgttgaagca actatgtgtt atacaggtga
     4261 tatactagat aagactaaaa ctaaatacaa tctagaatat tatattaaaa tggcacaaga
     4321 attagaaagc ttgggagcag atataatagc aataaaagat atgtcaggtc ttcttaaacc
     4381 atattcagct tacacactag ttaaagagtt aaagaaaaat gtaaaagcac caatacattt
     4441 acacactcat gatacaagtg gaaatggtgt agcaacttgt ttaatggcgt cagaagctgg
     4501 agtagacata attgatgctg cattagaatc tatggcagga cttacaagtc agccatcgct
     4561 taatgctata gtagaggcac ttaagaatac agagagagat acaggaatag atttatttgg
     4621 atatgatgag ttaggaaaat attataagga tttaagaaaa gtttataata aatttgaaag
     4681 tgatttaact aattcttgtg cagaaatata taattttgaa ataccaggag gccaatatac
     4741 aaacttaaaa cctcaagctg atagtttagg attagttaat agatttgatg aagttaaaga
     4801 aaaatataaa gaagctaatg aagttgtagg agatataata aaagtaactc catcttcaaa
     4861 agttgtagga gacttagcta tctttatgac taaaaataaa ttagataaag acaatataat
     4921 agaagaaggt aaaaatttat cattcccaga ttctgttgtg gattactgta aaggtatgat
     4981 tggtcaacca gaaggtggaa taccaaaaga tttacaagag gtagttctaa aaggtgaaga
     5041 agctataaca gtaagaccag gttcattatt accagcagaa gattttgatg aaatagctaa
     5101 atatctaaat gaaaagtatg atataaatgc taatataaga aatgttataa gttatgcttt
     5161 atatccaaaa gtatatgaag attatatcaa acatctacaa cattataatg atatatctaa
     5221 acttgaaagt gatgtattct tctatggatt aaataaaaat gaagaatgtg aagtagaaat
     5281 agaagaaggt aaggtattaa caataagatt agttgaaatt ggtgaagtta aggaaaatgg
     5341 atttagaact ataggatttg agttaaatgg aatggttaga gaagtagaaa taaaagataa
     5401 gaatttctct ggaaaaatta ataatgttga aaaagctgat atgaatgacc ctcttcaaat
     5461 tggagcaagc ataccaggaa aagttataaa aataatggtt aaagaagaag atgaagtaaa
     5521 agcaaatcaa ccattaatcg ttatagaggc tatgaagatg gaaactataa tagttgctaa
     5581 gactgatgga gtaattaagt caatcaaagt aaaagaagat gatatggttg aagataaaca
     5641 attattaatg ataatgaagt aatttaatta aaataaatta aaaagcactc tgaaatgttt
     5701 aataaaaaat gttttagagt gtttttattt attttaatta aatatttgtt aaaaaaaaga
     5761 ggacaattac ctccagatgt agaaataaag ctagtaagga aaatgttatc taaaaattct
     5821 aaatatagta aatattttcc ataatatttc ttaggaaata acagcaaaga taaaatacat
     5881 aaatttaaaa ggtgggtgct tatatgaagg tttatgatag taaaatgttg agaaatgtag
     5941 cagtattagg acatagcgga tgtggaaaaa caaatctaat cgaaactata gcttacacag
     6001 caaacactaa taagatacct aaattaacag acaaggtaaa tatgacttat agcatgggat
     6061 taatacctat agagtataat gattataagt ttaacttatt ggatacccca ggatattttg
     6121 actttagcgg agacgttgtg tcatctctta gagcaagtga tgcagcaata atagtaatag
     6181 atgcaacagc gcctattcaa gttggtacag agaaatcatt agaactaact gagagtattc
     6241 ctaagataat gtttataaat aaaatagata atgaaaaggc aagatacaaa gatgcaatag
     6301 caatgttaag agaaaaatac aataataaga tagttcctat gataagtcct atatacaaag
     6361 ataaaaactt tgtaaagtta cataatgttt ttgaaaacat agatgattta gaaggtgaat
     6421 ttaaggaaca agctatgagt gttaaagaag cattaatgga acttatagca gaaactgatg
     6481 accaaatatt agataaatat tttaatggag aagaactaac tacggaagag atacaaaaag
     6541 gtattattat aggtatacaa agaggtgata ttatacctgt aatatgtggt tcaactataa
     6601 ataatatagg cacaaaggaa atattagata caatatcaag ttatttagaa cctatcttta
     6661 cagaggaaag caaaccattt aggggattag tatttaaaac tatggttgac ccatttgttg
     6721 gaaagatgtc atatataaag attactgagg gtgtactttc taaggataaa gatgttttta
     6781 atataaataa aaatgtaaaa gaaaaaatag ctaatatata tacattgaga aatagtgaat
     6841 tagtagaaat agaaaaagct aaagctggtg atatagtagt tataactaaa gttaattctc
     6901 taaaaacagg agacactata tctgcagata aagatgcaga agctttagaa aagatagatt
     6961 ttcctaaacc acaaatatat tatgcagtaa ctcctaagaa taaaggtgat gaagaaaaag
     7021 tagcatctgt attaaataaa ctagtagaag aggaccctac attacattgg tatagaaata
     7081 cagagacaaa acaagcactt ttaggaggtc aaggagaact tcatataaaa acaattaaaa
     7141 ataaaatgaa agataagttt ggtgttgatg ttgaattgaa tgaccttaaa gttccttata
     7201 gagaaactat aaaaggaaca gccgatgtac aaggaaaaca taaaaagcaa tcaggtggtc
     7261 atggtcaata tggagacgta aaaataagat ttgaaagatg tgaatctgat tttgaattta
     7321 cagaagaaat atttggtggt tctgtaccaa aacaatatat accagcagtt gaaaaaggtc
     7381 taaaagactc tatgcaaaaa ggtatattag caggttatcc tgttacaaac ataaaagcta
     7441 cgctttatga tggttcatac catgatgtag actcttctga aatggccttt aagatggcag
     7501 caagtgcagc ctttaaaaaa ggtatggaag aagcgcaccc aatactacta gaacctataa
     7561 tgaaacttaa gataactgtt ccagaagaat atatgggaga tgtcatgggt gatataaata
     7621 agagaagagg aaaaatattt ggaatggaac cagatgacaa agggaaacaa ataatatttg
     7681 cagaagctcc acaagcagag acatttaaat atgcaataga tttaagagca atgacacaag
     7741 gtagaggata ctttgaaatg gaattagaga gatatggaga ggttccatca cagtttgcag
     7801 aaaaaataat tgggttagct actgcaaagt aatgaacaaa ataaatagaa aaataattaa
     7861 tatatattat taaaaatatt tttagggttt taactaggta taggtttatg ttaggtaaac
     7921 ttatacctag tttttataca tttttgatga ataaaaaaat atataatact ttcttaaatg
     7981 ttttaataaa aagaaagtat ttttttatta ttatttttac aaatgtttag taataaagat
     8041 gagaaggtat atttaaaata taaagtattt aaagttctga aatctaatc
//
LOCUS       DAEPOZ010000169         8022 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.169, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000169 DAEPOZ010000000
VERSION     DAEPOZ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8022
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.169"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            140..814
                     /locus_tag="KR440_002770"
     CDS             140..814
                     /locus_tag="KR440_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBH0699787.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKRKQNVKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            840..1676
                     /locus_tag="KR440_002771"
     CDS             840..1676
                     /locus_tag="KR440_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699788.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            1692..2363
                     /locus_tag="KR440_002772"
     CDS             1692..2363
                     /locus_tag="KR440_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0699789.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLVNQNIY
                     DLVILDLMLPNINGFDILKKMRVNNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            2353..3585
                     /locus_tag="KR440_002773"
     CDS             2353..3585
                     /locus_tag="KR440_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0699790.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            3735..4052
                     /gene="trxA"
                     /locus_tag="KR440_002774"
     CDS             3735..4052
                     /gene="trxA"
                     /locus_tag="KR440_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBH0699791.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            4069..4947
                     /locus_tag="KR440_002775"
     CDS             4069..4947
                     /locus_tag="KR440_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBH0699792.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            5217..5585
                     /locus_tag="KR440_002776"
     CDS             5217..5585
                     /locus_tag="KR440_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBH0699793.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            5588..5959
                     /locus_tag="KR440_002777"
     CDS             5588..5959
                     /locus_tag="KR440_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HBH0699794.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(6103..6558)
                     /locus_tag="KR440_002778"
     CDS             complement(6103..6558)
                     /locus_tag="KR440_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699795.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(6597..7862)
                     /locus_tag="KR440_002779"
     CDS             complement(6597..7862)
                     /locus_tag="KR440_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HBH0699796.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
BASE COUNT         3273 a          857 c         1291 g         2601 t
ORIGIN      
        1 aaaaataata atttagatga tttcacttat aataaaatgc tacaaaaaat aattgaatca
       61 aatagagtag atttagaaaa gaagaatata ataagaagta tgaaaagaaa gacaagaaga
      121 tgttgacgga ggaatattaa tgaataacaa tgaagacaga attgacaaat ttttagaact
      181 tcaaagcaaa ggatttaaat ttgcagagat agctcaaaat ctaaagatta gtcaaagtac
      241 tttaagaact tttttaaata aaaaaggata taagtcagaa aaaggtaaat acatattaaa
      301 agacgaatta aattataata aaagtttgga taatagaaac ttggaagaag agaaagctaa
      361 ggagtatgaa aaaagtaaaa agaatactat aagtaataga aaaaaagtaa gcaccaaaaa
      421 aaagatttct aatgaaaagg ctttaagtaa aagtgttgaa caaattgagt ttaatgatac
      481 aggtttaaaa aatgcaaagc gaaaacaaaa tgttaagcct aagaaagata gaaaaataaa
      541 tataactcaa gaagatttag ataagctttg tgaagtatat gattggtatt tagaagtaaa
      601 agattacaat ataaagccta aaaaagtatc taataaaaaa gatgtacata tagatgatga
      661 acacatagat gaattaaaaa caacaagaat acgtatagat aaagaaatat ggaatgattt
      721 tgaaagatta tgtagtaatt cgaattttga aaaacatcaa atacttacac aggcattgaa
      781 agaatttatg aaaaagtata aaaatctact ataaaatact actatatgag gtgcttaata
      841 tgaataaaat agtaaaaaaa tcaatttctc taatgattat attaacaata tttattttta
      901 tgttgacagc atgtgaaaaa gatgaacagc ctgattcagt tgatattcaa acagaagata
      961 aaaatgagat aaaaatagat gagggaaatg caaaagtatt gaatatagga aaatcagaaa
     1021 ttataaatat agatgaaggt gataaagtag atacatcaac aaaggtagaa aataattcaa
     1081 catttaatat atccaatgtt gaattgatat ataatgaata tgatgagaat aaaaaaatta
     1141 tatcttcgga tagtaaggct ttgttggata tgactttaat gcctgggaaa gttgcttaca
     1201 tagaatgtgg gcataaaact tttgtaaagg gtgtagaagt ttatgcatat gaatatgagg
     1261 cagagggtaa gattgtgtat gttaatttaa aagaaaatac tattaatata agaaataata
     1321 atattaagct agaaaatagt agtcagtatg aggtgctatc tacatcagaa cttaaaaaag
     1381 taaatgaatc aaatgaggga attacgtatc aagtaaaagt aaaaaattca tcatcaaaag
     1441 atttaggaaa tattattctt aagacagcag aagttaatga aaatggagaa tatttaacag
     1501 taaatcgtgt accatcatat aagattttaa agccttcaga agagacagat atagatatat
     1561 tgtgttcaac aaaggcaaaa agtgttgaaa tagtgggata tacgtatgat gatataaaag
     1621 aaaaagccaa tgtggatatt gacttaaaat ctcataaagt taagatagac aaataaaggt
     1681 aggggatact tatgaaggtt ttaatagttg aagataataa gatactttta gaaagtgtag
     1741 tagaagaact tagcaagcat tttgaaactg aaaagtgtga agatggagaa gaagctttat
     1801 acttagtaaa tcaaaatata tatgatttag ttattttaga cttaatgctt cctaatataa
     1861 atggatttga tatcctaaag aaaatgagag ttaataatat agatacacct gtacttatat
     1921 tgacagcaaa agaaacatta gatgacaaag tagaagcatt tactatagga gctaatgatt
     1981 atttgacaaa acctttttat atggaagagt tagtagctag ggtatacgct atattaagga
     2041 ctaatggaaa gataaaagaa agaaacggat tagaatttaa atctttatat ttagatacac
     2101 tagaaaaaag agtgtatatt gaaaaagaag aaataaaact tcaaaacaaa cagtttaact
     2161 tgcttgagta ttttgtatta aataaaggtt caattttatt aaaagagcag atttatgata
     2221 gaatatgggg aattgattca gatgcaacta ttgaaatagt ggaagtatat gtaagtaatc
     2281 taagaaaaaa gctaagtaaa tatggatatg ataaatatat taaaactaaa cgtaaggtag
     2341 gatatatatt cgatgataaa taagaatgta tttaccagta ctaaaaatca tttaataaaa
     2401 atgtatatta ttgtagtagg tagtttttta ataatctttt ctatatttat atactcatat
     2461 tttaggggac ttacatatag tggaattgat agtgagataa atgatgaact tgaatatata
     2521 gtctctcaat ttaaaagaac ttcatttttg aacccaataa gactgaaaga tcctaaagat
     2581 atggtgtatg tatatgaaga tggaaggata agttactata ctcaaaatga atattttgat
     2641 gagttacttc cagatagaag actagataaa aaaaattcgt tttttaaata cacagaaaat
     2701 ggatatactt ttagagaatt aaatgtagat gtaggtagat accaaataca gataattaga
     2761 aatatagatt ctgagatgaa ttctttaaga cagcttacat ctgttcttat aattggaata
     2821 ttaatttctg taataattac ttactttgtg gctgtatatc tcactagaaa agctttaatt
     2881 ccaatagaaa cagcatggaa aaatcaagct aagtttatac aagatgcttc tcatgaatta
     2941 agaacaccaa taacaatagt ttcatcaaaa ttagaaagta tgttaaaatc cccagaaagt
     3001 acagtaaatg atgaagttga aacaatagct actgcaatga aggaaactag aagacttaaa
     3061 aaaatgataa ctgatttatt gtctcttact aaagaggact ctattgttaa agttaattta
     3121 gaagaaatag acttagaaaa attattggaa gaaatatcag aagactatat tgacattgct
     3181 gagtttcagg aaaaaagatt cgtgtttaat tctaaactga agaataaagt tataataaca
     3241 gataaaaata aattaagaca gcttatatta attttcattg ataatgcctt taaatataca
     3301 aaattgggtg atgagatagc attagaatta aaagaagata tagaggatga ggtaacttta
     3361 cttataagtg acactggtat aggcataaaa aaagaagaga tacctcttat atttgataga
     3421 ttttttagaa gtgaaaatgt aagaaataaa gatttagaag gttctggaat aggactttca
     3481 atagctcgca tgattagttt aaacttatct attgatataa atgtaacttc tgatgtagat
     3541 attggaacta catttgaatt gtcaatacca aaaaagttaa aataataaaa atataaaaaa
     3601 ttgtttttag aaatgaagaa tgggtatact aattagtata aaatataaaa agaccttata
     3661 aaagtaaatt tatatttata aatttcatag tataattata tagggataat aaagtttaat
     3721 aggaggattt aaaaatggca aaagttataa atacatctga atttagaagt agtgttgaag
     3781 gtagtaaggg agtagttgta gttgatttct ttgcaacttg gtgtggacct tgtaacatgt
     3841 tgggacctgt atttgcagaa ttaggtgaag agatgaagga taaagctaga tttgtaaaag
     3901 ttgatataga tgagagctta gaaatagctc aacaattcaa tgtatctaca gtaccaacta
     3961 tgattatatt taaagatgga aaaccagtag aaactttaat tggattcatg ccaaagaaca
     4021 aaattgaaat gcaagtaaaa tcatatttat aaaaaatggg tgagtattat gagatatgat
     4081 atagctataa tcggcagtgg tccagctgga ttgtctgctg ctataaatgc aaaaataaga
     4141 aataaaacaa ttatcatgtt tggtaatgat aatttaagta ataagcttgt taaagctcct
     4201 tcaatagata attatttagg tttttatgat ataagtggtg atgaattaaa agacaagttt
     4261 aaatcacata tagatagtat ggatatttca attgaaaata aaagaataaa taatatttat
     4321 gctatgggag aatactttac aataatgtct ggaaatgata tgtatgaagc tactactgta
     4381 atccttgcaa ctggggtaga gtatactaga ccaatcaagg gtgaagaaga atttctagga
     4441 agaggtgtag gctactgtgc tacatgtgat gcaccacttt atagaaataa aaaggtagca
     4501 gttataggat ataatgaaga atctaaagaa gaagcaaatt ttttaagtga actcacttca
     4561 aagacttatt ttatacctat gtacaaaaaa gataatctta tgagaagttc agataaccta
     4621 gatgattcta ttgaagttat acatgacaga ccagtacaga tagatggaga taaacttgta
     4681 aacaaagtat cttttaaaga aaaccacatt gaagttgatg gagtattcgt tataaaagat
     4741 agtacggctc caagtgcatt ggttcctgga atagagattg atggcattca tattaaagtt
     4801 gacaataata tgaagactag tatagatgga tgctttgctg ctggagattg tgttggaaaa
     4861 ccctattctt atataaaagc agctggtcaa gggcaaatag cagctttaaa tgctgtttac
     4921 tatttagata aattaaagag ggcttaatat ttatattgat taacttttta aagatattta
     4981 taaaaaactg tcttgaaata attttattat ttcaagacag ttttttattt ttaagttatt
     5041 aataaaagta ttttaaaatc tatatgtaat tacttttatt aataaaattc aaagttatat
     5101 tagtagaaat tggaattatt tataatatac tccaaaattt aaaaataatg gttgaaaaat
     5161 ctaattaaga gtactataat ttaattaagt aattacttaa tcaaaacgag gtacttatgg
     5221 aaaacttggt taaaatattt aaagcattga gtgatgaaac tagacttaac atacttatat
     5281 tagtatccaa gagaaatata tgtcaaaagg ggatttctaa atatctaggg atttcagatt
     5341 ctgctgtttc acaacatata aaaatattaa aagatgttgg gataataaca ggatataaag
     5401 aaggatacta tgttttatat catattaata aagaatcatt taatatatgt gtagatttta
     5461 taaattctat gttaagtaat tctagtgaaa gctttataga tgtatttgat gtaaatacaa
     5521 tacatctagg atgcagtaaa gaatgtaaat ctataaaaaa atgttgtaag agaagggaga
     5581 aataataatg aaagtatgta taccagtaga ggagaataaa ggattggata gtaaaccata
     5641 tggacatttt ggttctgctc caatttttgt tgtgtgtgat ttagaaagtg gagaagttaa
     5701 atctttagat aatggagatt tagatcatga acatggaaaa tgtcaaccat taaaagcatt
     5761 gtcagggaca gctgtagatg cagtagttgt tggaggaata ggacaaggag ctataattaa
     5821 attaaatgga atgggaataa aagtttacag agcagaaggt gatactataa gtgctaattt
     5881 agacttactt aaaaatggaa aattagtaga gtttccatca gaccatactt gctcacatga
     5941 cggatgtgga catcattaaa aaaaacagct gatgaaatat atacttcatc agctattttt
     6001 tcttttaata aatacgattt tattctttta aaaccaaaat ttagattttt atgttttttg
     6061 tagtatcctt aataatattt caagtttaca agtttactac ttttagattt tttttgtatg
     6121 tttttctata tagtcaatat catttaatat gtcattgagt tctttattaa gtgtttttaa
     6181 atcatcacca tctacattgt agtttaaaaa tagttcctct acattatcaa tgatagaatt
     6241 gacttttcta gtaacttcaa caaatgtctt aaagttacta ctatttttag catttagaaa
     6301 tttttttaaa tcctttgata taaatgatac tatttcatat tcatcattaa atagacctct
     6361 accaatagga tatggttcaa ttctctgtaa aaaatattgg tctttagcat taatattata
     6421 agtataagat atttcccaac cgtctcctaa atcaaacttg ataaaatata atgtactact
     6481 tacttttgat attcttgtgg caccaagctg tcttaaaaga acttcaatcg aatcaccttg
     6541 aacagtatta atattcatat atacacatcc cctttagtat tattatacta tattttttaa
     6601 aattttataa ttaaataaac agtagatata ataatagata taatcataag tggaaaacct
     6661 attttgaaat actccttaaa agagatagga tgaccgtgtt tattacctat acctgctagt
     6721 acaacattag cagatgctcc tattagtgtt ccatttcctc caagacaagc tccaagagat
     6781 gttgcccacc atagtggcat tacatcaata ccttcagctt gcatagtcaa aataagagga
     6841 attagtgttg caacaaatgg aatattatct aagaaagaag agactatagc tgataaccat
     6901 aatatcaaca acattgtaaa tacaagatgt ccttctgtaa gtccaatcaa tgcttgagcc
     6961 aatttgttta ttatcccaac ttctactagt ccaccaacta ctacaaataa tcccataaag
     7021 aataaaattg tggaccattc aatacctgcc ataatttcat ctacatcttg ttttcctatt
     7081 aaaagcatta tagatgctcc tgtcagtgct acaacagaag aatctatgtg gattgtactg
     7141 tgaaacataa aaccaaaaag tatgaatgct attaatataa ggctttttat aagtagaggt
     7201 ttatctttaa cagatttttt ttcatctagt tttaatattg catttttagc tctttcattc
     7261 acaacaagtt cttttccata aataaatcta aagcatatta tagttatagc aagtattact
     7321 ataacagcag gacctaaatt tattacaaaa tccataaaac taagattggc agcactacca
     7381 atcataatat ttggagggtc tcctataaga gtagctgttc ctcctatatt tgaagctaaa
     7441 atctgtgtta ttaaaaatgg aactggatta agtcctaata tttgtgtaat aactatagtc
     7501 attggtccaa taagtaagac ggttgtaaca ttatctaata cagctgataa aatagctgtt
     7561 ataatagcaa aacaaatcat tatcttccat gggtcacctt tagctttctt tgcagtccag
     7621 atagctatat attcaaataa ccctgagttt ttgacaacag ctacaaatag catcatacca
     7681 actaatacac caatagtatt gaaatctata tggcttaatc cttcatcaag tgtaagtata
     7741 ttgaatatta tcattaatag tgcaccacca acagctgcta cggatctgtt taccttttct
     7801 gtaataatag ctgccataac tattaaaaat atacatatag ctattatttg ttgaaattcc
     7861 ataatataag tacctccatt tgtattattt tgtgcctaat gctagtacta tatatagtat
     7921 tactttttta ttgttttttc aatggatgta tgaaagtata ttatttaaca aagtttttga
     7981 gaaattgaca gaatttaaag aaaatttttg ttatttaaat aa
//
LOCUS       DAEPOZ010000170         7920 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.170, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000170 DAEPOZ010000000
VERSION     DAEPOZ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7920
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.170"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     misc_binding    158..371
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            625..1659
                     /locus_tag="KR440_002780"
     CDS             625..1659
                     /locus_tag="KR440_002780"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBH0699797.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKNI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEVL
                     PLDKVASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     gene            1701..2918
                     /gene="argJ"
                     /locus_tag="KR440_002781"
     CDS             1701..2918
                     /gene="argJ"
                     /locus_tag="KR440_002781"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HBH0699798.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKENDVLVASTGVIGKPLNINLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            2933..3793
                     /gene="argB"
                     /locus_tag="KR440_002782"
     CDS             2933..3793
                     /gene="argB"
                     /locus_tag="KR440_002782"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBH0699799.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGMDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKENE"
     gene            3823..5010
                     /locus_tag="KR440_002783"
     CDS             3823..5010
                     /locus_tag="KR440_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HBH0699800.1"
                     /translation="MNNNNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVVLKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            5119..6141
                     /gene="argF"
                     /locus_tag="KR440_002784"
     CDS             5119..6141
                     /gene="argF"
                     /locus_tag="KR440_002784"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBH0699801.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAYVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQKIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            6268..6885
                     /locus_tag="KR440_002785"
     CDS             6268..6885
                     /locus_tag="KR440_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HBH0699802.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     TVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
     gene            complement(7105..7803)
                     /locus_tag="KR440_002786"
     CDS             complement(7105..7803)
                     /locus_tag="KR440_002786"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBH0699803.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHELMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
BASE COUNT         3194 a          768 c         1442 g         2516 t
ORIGIN      
        1 atttcttaag ttgatgaatg ataatataaa gattataact caatttagat acaaaatgct
       61 ggaaattaac catatagaaa atatttccaa aaatttaaaa attatattga caaagttaaa
      121 aaaataaact atcatataaa acaataagaa aataaattga aatcctgtga aaaagagagt
      181 agcataaagc tagttgatag agagctggga atggtggaag cctagtaacg aattttattg
      241 tgaatagagc tttggaggag attaccagaa gtaatagtat ggtaattggt gaatctcgcc
      301 ttaagagatt agagtgggta agattatctt atcaattaga gtggtaacgc gggtgtatat
      361 tcgtctcttg gtctttgacc aagaggcttt tttaatttta aatgtacatt ttgtagatac
      421 tttttaattt gagttttaat gattattgta attttaccaa tgagagtggt accgcggaaa
      481 atcgcctctt agtatttata atgctaaggg gttttttgtt ttaattagaa tattttaatc
      541 tacatattat caaatgatta tcatatagta aagtggaaag atagaatttt aatactattt
      601 gaatataaaa atggagggaa caaaatgaat aaaataaaag ttggtattat tggcgcaact
      661 ggatatgtcg gggcagagtt gattagatta ttaatgaatc atgataaagt cgaagtgaca
      721 gcaataggtt caaattcgta tgttggaaaa aatatagttg atatatatcc aagtatagga
      781 tataaaaata acatgatatg tatagaaaat gaaaaagtta tagatatgtg tgatgttgtg
      841 tttacagccc ttccacatgg agttagtgaa aagtttgtaa taaaagcaat taaatcgaaa
      901 aagaaagtta tagatttagg tgctgacttt agaataaaag atgaagaagt atatagtaaa
      961 tggtatggag tatcttttat agataaaatc ttacataaaa aagctgtata tggattaagt
     1021 gaaatataca aagaagatat aaaagatgca gatataattg caaatcctgg atgttatcca
     1081 actagtattt ctttaccatt gatgccatta ctaagttcta aattgataaa aaataataat
     1141 ataataattg attctaaatc aggtcttaca ggtgctggta gagaattatc gattagtagt
     1201 cactttacgg aggttaatga aaatatcact gcatataaaa tagggaaaca cagacatact
     1261 ccagaaatag aacaaaattt aagtgaatcc tgcaaagaaa aagtaagtgt agtttttaca
     1321 ccaaacttaa taccagtaaa taggggaatc ttatcaacta tatattgtac aaaagaagat
     1381 aatatttcta taaatgatat acatagaaaa ttaactgatt attatgaatt taaagaattt
     1441 atagaagttc tcccattaga taaggtagct tctttaaaga atgttaggtt ctcaaataaa
     1501 tgtgtcatat cattacatga aaatggagat acattgataa tctgttcagc tatagataat
     1561 atgattaagg gagcagcagg tcaagctata caaaacatga atataatatt tggtattgaa
     1621 gaaaatacag gtttaaaaaa tattgcacca tcattttagt aatgaaaaaa tactaaactc
     1681 atataattag gaggataaat atggagatta taaagggtgg agttacagtt tcagaaggat
     1741 tttttgcttc agggattcac tgtggcttga gaaaaaataa agaaaaaaga gatttagctt
     1801 tggtttattc agatgtttta tgtgatgcag cagcagtagt aactcaaaat aaagtaaagg
     1861 gcaatcctgt ttatgttaca caggaacaca ttaaaaatgg aaaagcacaa gctatcatag
     1921 taaatagtgc taatgctaat acctttaatg gaaaagaagg acttataaat gcttataaaa
     1981 tggctaaatt cactagtgat aaattaaata ttaaagaaaa tgatgttttg gttgcatcta
     2041 caggtgtcat aggaaagcca ttaaatataa atttaataga agaaaatatg gatgaattag
     2101 tgaataatct aagtaaacaa ggtcatattg gagcaagaga agctattatg acaactgata
     2161 ttataaaaaa agaaatagca gtagcaataa tgtatggaga taaaaaaata actattggag
     2221 gtatggcaaa aggttctggc atgatacatc ctaatatggc aacaacgtta ggatttataa
     2281 ctacagatgc aaatattgat ggagttttgc taaaagaagc tttgaaaatt gctgttgata
     2341 aaagttttaa tagagttagt gtagatggag atacaagtac taatgatatg gtgatgattc
     2401 tagcaaatgg aaaagcaaaa aatgatagaa taaacaaaaa agatgaacat tatcaagtat
     2461 ttttgagtgc acttacatat gtctgtatag aacttgctaa acttgttgca aaagatggag
     2521 aaggatgtac aaaactaatt gaatgttata taaatggagc attaagtgaa gaacatgcag
     2581 taaaattagc aaaaacagtt atatcttctt cattagtaaa gacagctgta tttggagctg
     2641 acgcaaattg gggtagaata ctgtgtgctt taggttatgc aggtgaagaa atagatatgg
     2701 aaaaagttga tattattttt gagagtatga aaggatatat tgaagtatgt aaaaatggaa
     2761 atggattaga ttttaatgag gaaaaagcaa aaaaaatcct tgaagatgac gaaataagta
     2821 tattagtaga tttaaatatg ggcaatgcca gaggaaatgc atggggatgt gatttaagct
     2881 atgattatgt aagaataaat ggaagttata gaaattagga ggatatactt taatgattaa
     2941 tatagaaaaa gcaaatacac ttatagaagc tttaccatat atagaaaaac atcagggaaa
     3001 gactattgta gttaagtatg gtggaagtgc aatgaaaaaa gatggtctaa aagaatctgt
     3061 tatggaagac cttgtattaa tgagttatgt tggaatcaat atagttttag tacatggtgg
     3121 aggggcagaa atcaataaga tgctagcaaa agtggatata gaaagtaaat ttgtaaatgg
     3181 tcttagatat acagatgaag agaccatgga aattgtaaaa atggtacttg ctggaaaggt
     3241 taataaagac ctagtaaata aaatacatac aaaaggtgga aaagcagtag ggctttgtgg
     3301 aatggataat aatatgattt tatgtgaccc atataaaaat tatgaacttg gatttgtagg
     3361 agaaatcaaa aaagtaaatg ttgagcttat agaatcttgt ctaaagtcag gttatatttc
     3421 agtaattgca actataggtg ttggagatga tggagagact tataatataa atggtgacac
     3481 agcagcttct gctatagcta aagagttaaa tgcagataaa ttgatacttc ttacagatgt
     3541 ccctggtttg ttaagagagc cagatgaaga aaaatcatta ataacagaag ttatattaga
     3601 ggatgttgat aaattatttg aagaaggtat aattactgga ggtatgatac ctaaaataga
     3661 gggatgcgta gatgccttaa ataacggagt aaatagggta catattttgg atggaagagt
     3721 tccacattct ataataacag agctatttac tgatagtgga ataggtacat taataagaaa
     3781 agaaaatgaa taaaattata agtttaagag gggagaataa gaatgaataa caacaataca
     3841 atttcaaaat ggaatgaata ttttatagat acatataacc aacccaattt tgtcatagat
     3901 tatggtgaag gaagttgttt ttttgatact aatggcaata aatacataga ttttacaagt
     3961 ggatatggag tatcttctct tgggtatagt aatagtaatt taaagaatgc tctaaaagag
     4021 caagtagata aattattaca tacttcaaat ttatatttta atgagccagt gttgtattca
     4081 ggagagaaaa taattaattc aagtggaatg gctaaagtat atttttgtaa ttctggaaca
     4141 gaagcaaatg aaacagcatt caaaatagca agaaagtata gtagtgacaa atatggtaat
     4201 ggtagaggaa ctatcataag tttaaaagac tcttttcatg gtagaacaat gatgtcttta
     4261 atggcaactg gtatggacaa ataccataag tatttttatc ctcttcccga aggttttaaa
     4321 tatatagaaa gaaataatat tgaagattta aaaaataatc ttgattccac agtttgtgca
     4381 ataattcttg aagcgattca aggcgaaggt ggagttaatg ttcttgaaaa agattatgtt
     4441 ttggaaatag taaaaatatg tcaagaaaag gatattgtag tgatttttga tgaagtccaa
     4501 tgcggtatag gaagaactgg taaacttttt ggctatgaat attttgatgt aaaaccagat
     4561 atagttaccg tagcaaaagg gttgggagca ggaataccag taggaggagt attggtaaat
     4621 aaaaaacttt caaaagtact aggaaaagga gaccaaggta caacttttgg aggtaattta
     4681 ttagctatgg tggcagcttc agttgtatta gatgagatat ctaaagatgg tttttataat
     4741 gaagtattag aaaaaggaaa ttatataaga aaatctattg aaagttttaa taataaagtt
     4801 gtattaaaga caaaaggtat tggtctaatg ataggaattg aaactaatat tgaatctagt
     4861 attatagaag agaaagctag aaaaaaagga ttgcttattt tgactgctgg aaaaaatgta
     4921 ttgagatttt taccaccatt aacaatcagt tataaggaaa tagatgaggc tcttgaaatc
     4981 ctaaaagata tattactaga gattaactaa atattcaaaa aatagttaat taaagataaa
     5041 aattagaagt aaataaaaaa gtaaagtgct aacaagttta attgaattta agaagtttat
     5101 aagaaggggc gaattaatat gaataatata agtttaaaag gtaagagttt tttgacatta
     5161 aaggatttta caaaagaaga gattagatat ttattaactt tatcaagtta tttaaaaact
     5221 aagaaaaaag ttggcataaa aggcgatttg ctacaaggaa aaaatatagc tttattattt
     5281 gagaaaacat caacaagaac tagatgttca tttgaagtag cagctcatga tgaaggagca
     5341 tatgtaactt atctaggtcc aaatgattgt catatgggta aaaaagagtc tgtatcagat
     5401 acagcaaaag ttctaggaag attctatgat ggaatagaat ttagaggctt taaacaagaa
     5461 acagttgaaa gtctagcaaa atattctggt gtacctgttt ggaatggact tacagatgaa
     5521 tatcatccaa cacaaatact ggcagatttt ttaacagtaa ttgaaaatgt agataaagat
     5581 ttgaataaag ttaaatttgt atatgttggt gatgctagaa ataatatggg aaactcactg
     5641 atgataggat gtgctaaaat gggaatacat ttcgtagcac tagcacctaa ggaactttgg
     5701 ccaaatgaag aattggttaa ggaaatgaag gaaatagcca agaaatccaa aggagatata
     5761 atgttgactg aaaatattga tgatgttaaa ggtgcagatg ttatatatac agatgtatgg
     5821 gtttctatgg gagaagagga gcaatatgaa gcaagaataa atcaacttaa agattatcaa
     5881 gtaaatatgg atatgataaa gaaaactgaa aatgaaaatg ttatattttt acactgtctt
     5941 ccagcatttc atgatttaga aactaaggtt gctcagaaga tttatgaaaa gttcggatta
     6001 tcagaattag aggttacaaa cgaggtattt aattcaaaat attcaaaggt atttgaagaa
     6061 gctgaaaata gaatgcatac aataaaagct gttatggttt ctactatgac tgatgtaaat
     6121 atgaacaata atttaatata gatatgtttt aatatagact ttactttaat gaaaatttat
     6181 aaaatatttt aaatatatta ttcaaaaact ctcattatat gttaaaataa cttatgtatc
     6241 aacaaattaa gagggaggat ttgagatatg aaaaaaatca tattagcttt aacaactttt
     6301 ttattagcaa tatctttaac agcatgttca agtgcaaaac cagaagatac aattgatagt
     6361 ttttttaaca gtgcgaaaaa gtttgacttt gaaggcatga ataaggtaat ggaaaataat
     6421 gacgaaaaat ataaggatat attaaaggaa ttagatacaa aagacccaaa tgcccaatat
     6481 gttttagatt atctaaaaca gaatgcatct aaaatcacgt atacaataaa agacagcgag
     6541 gttaaaggtg ataaggctac aatcaaagta gaatgtaaat ttatagattc aacaccatta
     6601 cttcaagaaa tagtagctga ggcatttaca aagatgattg gtatgagttt ttcaggacaa
     6661 gacttaactg atgagaaaac aactgaaatg ttagtatcaa ttatgaaaga aaaacaaaaa
     6721 agcgttaaag agacttatgt tacaaaaact gtagagtttg aatgttctaa aaaggataat
     6781 aaatggataa taagttcagt aaatgatgca gtagctgatg tattgttatc taatcttgtt
     6841 acagcaggac aagaattttc taactctatg aatacacttg aataatattg tttaggtgta
     6901 gtaaagtaat aaatatatga ttatttttat aaataaacat ataaatattt atgaaggata
     6961 ccatttttgg tatccttttt attaatataa tgaaatacat ggttaatttc tatattgtca
     7021 agaaactatg atatatgtta taagtctata ctatttaaaa taaacttata acatatattt
     7081 tttataatat agtgcatgaa aaatttattt aactaatttt agatatttat tgacaactga
     7141 ttcagccaag cattttgatg aatcaacaat atttattgaa ggatatgatt tttctaaaac
     7201 tacatttaaa tcagtacaag caattactat attttctata gattcatttt taacatcttc
     7261 gataagttta ttccaaatat caatattgtg tggattttct ttatccattt ttatattttg
     7321 aataagattg tttaatttta tttgccattc atctttaaat ataaattcat gtccattatg
     7381 tattatacca ttctggtaaa ttgtagactc aaaagttgaa ctagtcgaaa aaagagttac
     7441 tttttgtgag atagtaggta attttttaac tgtttcttct acaatattta atattggtat
     7501 attaatagac tcttttaatt ctttaaagta tatatgtgct gaattgcatg gcattgcaat
     7561 aaaatttaca ccagttgatt caagcttttg taatccatca attatagttt ctttcataag
     7621 ttcatgattt attggacggt caatataaaa tggggtaggt aatgaatata tcatcatctt
     7681 tggaaattca tcatcatatt ttgctccata ttgagattga cactcatcaa taattaaatc
     7741 aacaaaaggg gctgttgacc ttggacccat tccagccaaa ataccaatta atttttcatt
     7801 cataaaaatt atctccttta tatataaata tatttatgta taaaataaaa atctataaaa
     7861 gcataaaatt atattttata atagattata ttgtacactt gtataatata aatataaagt
//
LOCUS       DAEPOZ010000171         7829 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.171, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000171 DAEPOZ010000000
VERSION     DAEPOZ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7829
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.171"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            55..1122
                     /locus_tag="KR440_002787"
     CDS             55..1122
                     /locus_tag="KR440_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HBH0699804.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESVILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEESKDISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            1141..2301
                     /locus_tag="KR440_002788"
     CDS             1141..2301
                     /locus_tag="KR440_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBH0699805.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPVSMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEETELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVSTILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            2314..4410
                     /locus_tag="KR440_002789"
     CDS             2314..4410
                     /locus_tag="KR440_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HBH0699806.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSTKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGFNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNDLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTKEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(4569..4913)
                     /locus_tag="KR440_002790"
     CDS             complement(4569..4913)
                     /locus_tag="KR440_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBH0699807.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            5205..6095
                     /locus_tag="KR440_002791"
     CDS             5205..6095
                     /locus_tag="KR440_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBH0699808.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            6313..7599
                     /locus_tag="KR440_002792"
     CDS             6313..7599
                     /locus_tag="KR440_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBH0699809.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDICEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKKFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(7688..>7829)
                     /locus_tag="KR440_002793"
     CDS             complement(7688..>7829)
                     /locus_tag="KR440_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699810.1"
                     /translation="TFLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKK
                     KP"
BASE COUNT         3026 a          860 c         1402 g         2541 t
ORIGIN      
        1 aaagtataaa attttaaaaa ttgaattata acttcaaaaa ataaggaggg taatatgaag
       61 ataacagata tcaaatttga aaagcttaga attaaattaa aaaaacctgt agtagtttca
      121 tttggagtaa ttgaatatgg agaaagtgta attttaaaaa ttgaaactga tgaagggtat
      181 tgtggatttg gcgaagcagc acctcttgca gcagttacag gtgaagtttt ggataatgtt
      241 ctttcagtat tgcttatgtt taaaaaagag ttaataggca aagacccact agatattgaa
      301 accatacata caatcatgga tggaattatt ataggtaata cttctgcaaa agcagctatt
      361 gacatagctt tatatgatat taaaggaaaa attatgaatg cacctcttta taaagttctt
      421 ggaggatttg atagtaaagt ccaaacggat attactataa gtatagataa gccagaaaaa
      481 atggcagtag aagctttaga aagagtaaaa gaaggtttta ggatattgaa gcttaaagca
      541 ggtataaatc ctgaagacga tattgaagct gtaaaattaa ttagagaagc cgttggagag
      601 agtataagaa ttagaattga tgcaaatcaa ggatggaacg taaatagcag tataaatact
      661 atcaaaaaat tagaagaatt taatgttgat gcaattgaac aagcacttcc acattgggat
      721 ttagatggta ctgcttacat aagaaataaa agtaatacaa aaattatgat agatgaatcg
      781 ttacattcac caatagatgc aattaaggct ataaaaaaga atgcagttga tacatttaat
      841 attaagctga tgaaatcaag tggaatatat cctgctataa aaatcaacaa catagctgag
      901 gcaagtggag taaattgtat gttaggatgt atgctggaaa cgagaattgg tataacagca
      961 gcagcaaatt taatagcatc taaaaagaac ataacagaag ctgaccttga tagttttatg
     1021 ttttgtgaag aatcaaaaga tatatctgga ggatttgtaa tggatggaga tattatgaat
     1081 cttgtcaata aaccaggttt aggaatagaa gtaaacttat gataaatttg gaggttaaat
     1141 atgaatacac ttttttcaaa tccaggtagt atacttgctg taatgacatc aatgattgca
     1201 ttgggttttt acttacaaag atacaaagcc attaaaagct taggaccagc acttacaata
     1261 atcataatgg gaataatact ttcaaactta aaagtagttc cagtaagtac tgagttatat
     1321 ggaactattt caacatatgc tatcccagtt tctatgacta taatgttgat gagtgttgac
     1381 ttaaaagaaa tgacaaaatt atcaagagaa cctttaattg caatttttgt agctgtatta
     1441 actgtaagta tcatggcatt tttatttgga ttagtatttg cagaaaaaat atcagaaggt
     1501 tggaaggttg ctggaatgtt tgtaggtaca tacactggtg gaagtgctaa tcttacagct
     1561 attggaacag gccttaatgt aagtagacaa actcttgctg cagcaaatgc agcagactat
     1621 gtaattggag taccaacatt gatatttatg tttgctctac cagcaatatt aaaaaattca
     1681 aagaagttta aaaaactttg gccatatcat gtagaagaaa cagaattaga agattgtcaa
     1741 aatgaagaat ttatggagtc aaaagaatgg agtattaaag atatagcttg gatgttggct
     1801 ataggttttg ttgttacaga agtttcaaca atacttgctg gatatttcaa ttcaagtttt
     1861 agtagtgcag caagaatact tttagttaca acaatttcaa ttataattgc tcaattaaag
     1921 ccagtaaaga aactaaaggg aaatttagat ttaggattat ttgttgcatt attcttttta
     1981 tgtacaatag gtttttcagt agatataaaa gagtttttag gttcaacttt tactataaca
     2041 ctttactgtt ttagcataat ttttgcttca tttgtttttc atttaggcat aactagactt
     2101 ttaaagataa agtatcaata tgtaatactt tctatagtag gagcaattgc tgatggtcct
     2161 acttcagctt tagtagctgc ttctgcaaag tggaattcgc ttgtaagtgt agcagtcgta
     2221 atgggagtaa ttggtggagt attaggaaat tatgcaggta tatcagtggc atatgcaata
     2281 aagatgtctc taggattata ggggggagtt taattgataa aatttaaatt atacatatct
     2341 ggactataca gtggaaatgt aatctttgat ggcgatttat taattgaaaa gttgaatcct
     2401 tttacaaata aaatagaaag tttaaaacca ataagcaaag aagaaaatac atactattta
     2461 aatcttacaa aaatagattt aaagtcttta tttaataact ttgatgtata tactaaaagc
     2521 ttagtaaaca cagataaaga cacagttata aataacttag gagaaacgca ttctaaactt
     2581 aatgaatata tatgggttca aagaaacaaa aagttcccac tagatataat tattgtagat
     2641 aataaaatag taggttttat ctgtttatcc agagaaactt gtactatatt aattatggat
     2701 ggatatgaag agtatactgt attaaaagaa tgggaaaaaa cccataaaaa tgaagagata
     2761 tattctataa gatttggtgg aaattatatg attgatatga aagatggtat aaaactttct
     2821 acagatgtat atctccctga ttttgtagat tctaccaaaa aagcaccaac aattcttatg
     2881 agaactccat atggaaaaga aaatgataaa gaaatatatt ataaatatgt acaaagagga
     2941 tatgctgttg taatacaaga tgttagggga agaaatgaat cagaaggtaa gtgggaacct
     3001 atgattcatg agagggaaga tggagatagt accataaatt ggatagtatc acaggaatgg
     3061 tcaagtggaa tagttggaat gcttggagca tcatatcttg gttatgtcca atgggcagct
     3121 gcatcttcag gcaataagca tcttaaagct ttagttagta tagtaacatc aggaagccct
     3181 tttattgata taccaagaaa aggaggagct tttgtatctg gtatgcttgc atgggcattt
     3241 atggtatcaa gaaataaagt tgatagaagt aaaatggtaa gagatgattg ggatgatgtt
     3301 ttaaacatac gaccaataga aaatatacct gttgaagctc ttggatatag aattgaattt
     3361 ctggaagaat ggttaaagag agtagaaaaa gatgagtatt gggatttgat ggattggcat
     3421 ttacaaaaag ataaaataaa tgtacctgca ttggttgtat ctgggtggta tgatgataat
     3481 agtatgggaa ctacagaggc cttagatgtt ataaaggact atgaaaaagg aaagagaaag
     3541 gctattctag gtccatggat gcataattca aatactttaa gagatataaa tggtatttct
     3601 ttaggaaata gttcattaag atatgatttg gattataatt atctattatg gtttgataaa
     3661 tacttaaaag gtatagaaaa taacatagat acgactgctc cagtagagta ttactctgta
     3721 ggttttaata agtggaagac agaagaaaat tggccaataa taaataagat agataaaagt
     3781 atgtatctaa taagtgatgg aaatgctaac acatctttag gaaatggtag attagtattt
     3841 gataatgatt tagaagaaaa gtatgatagt tatatatata atccaaaaga cccatcagta
     3901 caactaattg atatgtcaga gaatgaagtt ggagttccaa ataactacaa agatttagaa
     3961 aaaagaagtg atatgttgtg ttatacatca gatgctttta gtgaagaatt tactgtaaca
     4021 ggtgatataa agttagagtt ttttgcatca agttctgcca aagatactga ctgggttata
     4081 aaaattatgg acgtggattt agatggaaat tcaattaaat tagctgatgg tatacttagt
     4141 gctagattta gaaatagttt ttataagtca gagtttatgg aagaaggaga gatttataag
     4201 tttacgatta taacttcaaa aatatcaaac acgtttaaag ttggtcataa aattagacta
     4261 gatataacgt ctagtgctaa aaactttatt ttccaaaata gcaacacgaa agagggatat
     4321 aatagtattg aatatataga agcaaagaat acgatttacc atggaggtaa atatccatct
     4381 aaattgatat tgccaataga aaataagtaa atttatatag attaaatagt gaggaggtgt
     4441 ctcaaaatga attttttagt tcgtgagaca ctttctttta tacgaaatca aatatatttt
     4501 catcatttca acaatgaaaa agaggcttat ctctctgttt tgagatagcc tcctataagc
     4561 taaattgatt attttgtagc tgcaacaacg ctcatttcaa ctaacaaatg ctctctagca
     4621 agtctagctt caacacaagc tctagcaggt tcaaatcctt tttcaaccca agcatcccaa
     4681 acagagttca tagcttcaaa gtctttcata tctcttaaat aaatagtaac agatagcaaa
     4741 tgttgcttat ctgaaccata tttgtttaat aaatcttcta ttttagctaa aacttcagtt
     4801 gtttgttcaa ttactcctcc ttcagcatga gtttgtccac ataaataaac tgtattgtta
     4861 tgaactacag ctctactcat tcttccagtt ccttcatatc tctttatttc catattttaa
     4921 atacctccat agttaagaaa tctattaaaa atctaaataa taaattaaaa aaagtctata
     4981 ctatataaaa ttatattata tattctgata ataatccata aaaacatata actttttagc
     5041 tataattatt attgttatta attgaaaaga cttattagtt acttgacata gtttacactt
     5101 ttaattatag tataaattgg aatatttaga taattattat ttaatgtata agctaagttt
     5161 ttaattaata tggctttata catttatata gaaaagaggt acgtatgagt agcacaatca
     5221 aatcaaatcg aataaaaggt ataatgttta taatagcatc agcgcttggc tttgcaatga
     5281 tgtctgcatt cgtaaagcta gctggagatt taccaagttt tcaaaaagtg ttctttagaa
     5341 atctggtatc agctataata gctttttggc ttatcataaa acataaagga agtctaactg
     5401 gtaaaaaaga aaatttaaaa gtacttttat ataggtcaat atttggaact ctaggagtta
     5461 tatttaacta ctatgcaatt gacaagttga tgctttcaga tgctaatatg ctaaataaaa
     5521 tgagtccctt ccttgtagta atattttgtg caattgctct aaaagaaaaa ataaatgtaa
     5581 agcagatagg agctattata attgcattta taggagcttt attcataata aaaccaacat
     5641 ttagtgtaga agtaattcca tatcttgggg gaattgcagg agctgtattt gcagcaatgg
     5701 catatacttg tttaagagtt cttggagata gagaagatta ctatacaatt gtatttttct
     5761 tttcagtttt ttcattagtt actgtagggc caattgcttt tgcagtatat gaaccaatga
     5821 cccttatgca acttatatat ctattactag gtggagtatt tgcaagtcta ggtcaatttg
     5881 gaataacttt agcatataaa tatgcacctg ctaaggaaat ctctatattt gactatagta
     5941 atataatctt ttcagcaatt ttaagtatat tcttattcaa tgtctatcca gatatgttaa
     6001 gtgttgttgg atatttaata gtattttcgg cagcatttta tatgttttta tacaataaga
     6061 agttagataa attagacaaa gaaaagaaga agtaaactag ataaaagtgt ttttataatt
     6121 tgtattgaat atttaagtct ttgtattaaa attaaagaaa atttaattaa aaatgtttgg
     6181 tatattaact tataacagca gttaaaattg aaaaattata actgctgttt cttttattaa
     6241 aaatatccaa atgtctgtaa aaatagacta taatagagta gtataaataa tataaagggg
     6301 ataaattaaa gtatggaaaa agtgaaagtt gctgtgattg gagcaggtaa tagaggaacg
     6361 tatgcatatg ctccatatat ttatgaaaat tctgatatat gtgaaatagt tgctgtagca
     6421 gaacctaaaa aaggaagacg agaactattt acacaaaaat ataacctaga tagtaaaaat
     6481 gtatttgaaa ctttagaaga cttttttaaa catgacaaga tggcagatgc agtaataata
     6541 gctacaaatg atgatagaca ttatgatgta gcaaaactag ctcttgaaaa aggttatcat
     6601 gtactattag aaaagccaat gtctaatagt ttagatggtt tggtacatat agatgacttg
     6661 tgtgataagt ataaagataa gatttttatg atttgccatg tactgagata ttctccattt
     6721 tataataaat taaaagaaat tgtagaaagc aaaaaattag gagagctagt tagtatacaa
     6781 tataatgaaa atataggata ttggcatttt gcacatagct ttactagagg gaattggaga
     6841 aatagcaatg aaaccagccc tttaatttta gcaaagagtt gtcatgacat ggatattttg
     6901 ttatatttag taggaagtag atgcaaaaaa atatctgcat ttggaagctt aaagcatctt
     6961 aataaccaaa atgctagtgg agagatggca caaaattgtc ttcagtgttt agttgaaaaa
     7021 aaatgtccat attcagcaaa gagaatttat ttagaaaaag atagaagtat aaatagagct
     7081 gttcacataa atccaactga agagaatcta ttaaatatac ttaagacaag tccatatgga
     7141 agatgtgttt acagatgtga taataatgtt gtagacaata tggtaaatat attacagttt
     7201 gaaaatggag tgacggctac ttttaattta tgtgcattta ctaaagaaaa tggaagaact
     7261 ataaagttaa tgtttagtca tggtgaagtt ggtggggatt taaataaaaa tgagataaga
     7321 attaaaaaat ttggaaaaaa cgaagaaata gttatgaatc catcaaatca acagaatatg
     7381 gtagaggaat atgatagaaa tcttatagct gaatttatta aattagtttc aaataaagaa
     7441 ctagaaaagg gtagagtagc agcaaaagaa gctatacaaa gtcatgtaat ggcatttgca
     7501 gcagaatatt ctagggtcag tgatgaagta gtgtatatag aagaattttt tgatagtgct
     7561 aagcaaatga ctaaggagat agaagaaact atattttaat taataaaaat ttattaaata
     7621 aaataaagat agattaaata atagaaatac gaaaatatgt gttctattaa ggaatctatc
     7681 ttttttatta aggttttttc ttatccctat caaaaaaatc catacattct gataaaagat
     7741 aagaaagtaa agcgaataag atttctgtac ttaaaggaat cgttactgaa tccataaagg
     7801 tatcaattat gttcatagct agaaaagtt
//
LOCUS       DAEPOZ010000172         7814 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.172, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000172 DAEPOZ010000000
VERSION     DAEPOZ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7814
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.172"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            241..2412
                     /locus_tag="KR440_002794"
     CDS             241..2412
                     /locus_tag="KR440_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBH0699811.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNDTTSISVMF
                     KDYKKLNKNYSKWIKDIADENNVAISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNED
                     TINKILRELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVS
                     REASILRLNGYSKLDIIARVIKNIFRIMILSSVTALLIYIIYILKLNIGVRACLYFAM
                     FSIICILINILISILIVGFNSRSSKYVLSLNGKKSYGFVNIMHFTLKIVFVSFLILSI
                     NNCILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNLV
                     EEKDAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGK
                     EIFNLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRELNKT
                     KKSDLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIIDIDNIDDSFYLSYISRCV
                     YFNSKKLDALADISNIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIIIA
                     ISNLMITYNIVASYYERNKYKLYIKKIFGYSTTARSMLLIVSLILTNIIPIGIISTRV
                     DLSNNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            2417..3034
                     /locus_tag="KR440_002795"
     CDS             2417..3034
                     /locus_tag="KR440_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0699812.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKNLNKNGKTIVFVTHDLELSEQADRVIKL"
     gene            3223..3567
                     /locus_tag="KR440_002796"
     CDS             3223..3567
                     /locus_tag="KR440_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBH0699813.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
     gene            4137..5003
                     /locus_tag="KR440_002797"
     CDS             4137..5003
                     /locus_tag="KR440_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBH0699814.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPIDK
                     ESGKPCINLQPDFKCSVHNSLMKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSPELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMSPEQIINLD
                     IISHRTKVNLLLSQASESVMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRSTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
     gene            5116..5520
                     /locus_tag="KR440_002798"
     CDS             5116..5520
                     /locus_tag="KR440_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HBH0699815.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEAIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            5698..6147
                     /locus_tag="KR440_002799"
     CDS             5698..6147
                     /locus_tag="KR440_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0699816.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(6303..6680)
                     /locus_tag="KR440_002800"
     CDS             complement(6303..6680)
                     /locus_tag="KR440_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH0699817.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            6942..>7814
                     /locus_tag="KR440_002801"
     CDS             6942..>7814
                     /locus_tag="KR440_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HBH0699818.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWGTCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLL"
BASE COUNT         3187 a          793 c         1210 g         2624 t
ORIGIN      
        1 atactaattt tgtaccacta ttaaaatatt gtttgctaaa aataaaaaat aaccttaaaa
       61 cagttgatta taaaaaatta actgttttaa ggttattttt atctttgtga cgaaatagac
      121 acttgccatt tagaatgtga caatatagta gttgtttaga aaaggtacaa agcattatac
      181 tttaactaaa gtaaaatata ttttgctttt agaataaaaa caattaggag ggagctataa
      241 ttgaaaaaga taatatatgt attatttaca attgaactat taatagtatc tttattaggt
      301 ctaaatatag ttaaagataa tgaaattaat aatattttat ataacgatac aacaagtata
      361 tctgtaatgt ttaaagatta taaaaagtta aataaaaatt atagtaagtg gattaaagat
      421 attgcagatg aaaataatgt ggctatatca aagtatgttt ttaataataa tgaaaaatta
      481 tctatatata cgactgatac aagtttgaat aacaatataa agttaaaatc aggaaaattt
      541 cctaatacta attcaaatga atttattagt aatataaaca ctaataatag taataatagt
      601 aatcaagtag gtaaattctc aactatgagt aaagacatat caattttaat tagggatttt
      661 ggtaaaataa gtgaagttgg agaatcagga atcttatata tttcaactca aaatgaagat
      721 acaataaata aaatattaag agaactaaat gtagacagaa gtatttttgt agcaagatta
      781 catgatagat atgttaactc aaatttatac ttaaatcctt caataattag agatttaata
      841 ctaattatcc tttgtttctt ggctacaata atacattatt caattagtgt gagtagagag
      901 gcttctattt taagattaaa tggatactct aagctagata taattgctag ggttataaaa
      961 aatatattta gaattatgat tttatcatct gtcacagcat tattaatata tataatctat
     1021 attttaaaat taaatatagg tgttagagca tgcttatact ttgctatgtt ttcgattata
     1081 tgtattttga taaatatttt gatatctata ttaatagttg gttttaatag tagaagttct
     1141 aaatatgtgc tatctctaaa tggaaaaaaa tcttatggtt ttgtaaatat aatgcatttc
     1201 acattgaaaa ttgtatttgt atcatttctt atattatcca taaataactg tattttaaat
     1261 tataatatgc tacaaactca gttaggaaat ctatctgagt gggataaagc aaaaaatgtt
     1321 tataatctta ctcttaaaga tacaggcgaa caatctttag gaaaggaaga agtaattaaa
     1381 aatgccaaga ttaaaggttt ctataaaaat ttggtagaag aaaaagatgc tttcttaata
     1441 gattcaacta attttgaaaa acttgaagat ggaagttaca tgtatgaaat aaattcaaag
     1501 ggtaaaaacc cagaagtaag tcaatatggt aaaaatataa aaataaataa aaactatctt
     1561 aaagttaacc ctatttactc aaatggcaaa gaaatattta atctaatcga ttatgacgat
     1621 aagactataa atttattagt tcctaaaaag ttacaagtac atgaaaatga gattaaaaaa
     1681 gagtttagag aattatttta ttttgaaaaa atagaagtag aaaatatgta caataaagag
     1741 ttaaatagag agttaaataa aacaaagaag tcagatttaa atgttaatat aatatatgta
     1801 gataataatc aaagctattt tacttataac tcccttgtta tggatgacaa taggaatctc
     1861 attgatgacc caatagcaat aattgacata gataatattg atgattcttt ttatctttct
     1921 tatatatcta gatgtgtata ttttaattct aagaaattag atgcccttgc agatataagt
     1981 aacattattg aagctcaagg tgtagaagcg catatccaaa gtttacatgc tgtttataat
     2041 gagtatggtt tagaaataaa taaactagaa aaatatttga atagtgaaat ttttactata
     2101 ataataatag caatatcaaa cttaatgatt acctacaata tagtagctag ctactatgaa
     2161 agaaataaat ataagttata tattaaaaaa atatttggtt attcaactac agctagaagt
     2221 atgttgttaa tagtctcatt aatattaaca aacattattc caataggcat tatatcaaca
     2281 agagtagatt taagtaataa tattatctta tttggacttc tgatattagt tatagaagta
     2341 attgtatcta ttgtattaga caagattatc tcaaatagtt catttaataa aattataaaa
     2401 ggagaacatt aaaaagatga tttcattaat aaatatatct aaaaaattta aagataaatc
     2461 tataatagac aaatttaacc tagaaatttc taagggcgag tttgtagcga taacaggaaa
     2521 tagtggttca ggtaagacaa ctttacttaa tataatggga ttattagaaa aacctaattc
     2581 tggtgatatt ataataaata atataaaaaa tccaaatagt aaacagataa gaattttaca
     2641 aagggatttt tatggctatc tatttcaaaa ttatgcttta attgagaatg aaaatgttga
     2701 aaataacttg aaaattgctt taaagtatca aaaaaatatc aaccataatg aaaaaataaa
     2761 ttacgcactt gagtctgtag gtcttagaaa atacaataaa aaaaagatat acgaattgag
     2821 tggaggagaa caacaaaggg tagcattagc aagagtaata ttaaaagaat gtgaagtaat
     2881 atttgcagat gaacctacag gtaatttaga taaaaaaaat agagacatag tatttgatat
     2941 acttaaaaat ctaaataaga atggtaaaac tatcgtattt gtaactcatg atttagagtt
     3001 atcggagcag gcagatagag ttataaaatt gtaataatat caactataat aaatataaga
     3061 aagagattac agctaaatca agtttaataa taatacgaat agaatttaaa cattaagtaa
     3121 attagtagtt tgtaaatata agtataatat gatatcataa aggctcaaaa gaaattttat
     3181 ataataagaa atttaaaaat aacaaatgga ggacatataa agatggaaaa tttaccaaat
     3241 tgcccgaaat gtaattcaga atatacttat gaagatggaa ctcttttagt ttgtccagaa
     3301 tgctctcatg agtgggcatt aggattggga gctgaagaag attcaaatat tatcaaagat
     3361 gtaaatggaa atgtattaaa tgatggagat tctgttacag taattagaga ccttaaagta
     3421 aaaggaagtt catcatctat aaagattgga acaaaagtga aaaatatccg tttaatccat
     3481 gactcatcag atggacatga tatagaatgt aagatagatg gttttggagc tatgaagcta
     3541 aagtcttctg ttgttaaaaa agcgtaatat tataaataag ctgtagtgtt tattgttttt
     3601 cagtacagta taaaaccata agaatctatt aagtaataaa agtttgttta tttatcttta
     3661 tattttctta tggttttatt aaatataaat tttattgaac accaaaatat tctctcctct
     3721 acaaataagc caatcttatt taaattaata tactttgtac tatttatact gaaattgtga
     3781 atttaaatgc tatgttattt aggtttgtat agttaattta tatatttctg attttataaa
     3841 ttgtatttat tattatatct tgattctaaa atcattaaaa atttcaactt ctataaagat
     3901 ttacaattta tcaaatacat tatgagtata taaacaattt attattacag tgctacattt
     3961 aaaataagtc ttagtataaa ggtattgtta caagtataaa gaaacttatg tatgctattg
     4021 gaataaataa taccttaaat tataattaag aataagaact gttaaataag ggacttgatt
     4081 atcatttagt tatagataat ataatattaa aaataaagct taagaggaga aaaaatatga
     4141 tagatataca taaaaatata tatgataata agttatttga ggagctaaag attgattgta
     4201 agaagtgttt tggactttgt tgtgtagctc tttatttttc tgcttcagat ggatttccta
     4261 tcgataagga atcaggaaaa ccctgtatta atttacaacc agattttaaa tgttctgtac
     4321 ataatagtct tatgaagcga ggttttaaag ggtgtactgc atatgattgt tttggttcag
     4381 gtcaaaaagt agcacaagta acatataagg ggatagactg gatgcaatca ccagaattaa
     4441 caaatcaaat gtctgaagtg tttttaatta tgagacaatt acatgaaatg ttatggtatc
     4501 ttaaagaagc atctgtatta aatatatcag atacaatcaa atcaaaaatt gatttgataa
     4561 ttgaagaaac tgaaaagata actaatatga gcccagaaca gataattaat ttggatatca
     4621 tatcacatag aacaaaggtt aatttattat tatcacaagc gagtgagtct gtgatgggaa
     4681 aggttaaatc atttatcaag acttctactc ttaaaaatat gaaaaaatta agtaaaaata
     4741 tagatttaat aggagcagac cttagaaaaa taaatctaat aggagcagat ttaagaggta
     4801 gatttctaat agcagcaaat ttaagaagta cagatttgag tggggcaaat ttaataggag
     4861 cagacttaag agattgtgat attagaggtg ctaatcttga aaatagtata tttttaacac
     4921 aattgcaagt aaatactgca aaaggagatt ctagtacgaa gttaccagct tcactaatac
     4981 gccctaaata ctgggagaaa taattaaatt aatacataat tttacaaaat atgaactttt
     5041 ttgactttaa ttcatattat acattagaga ttctttacaa gaatttctta atataatata
     5101 gggagagata taaaaatgag ttatcaaaat tataaatgtg aaagagatta tgtgtatgat
     5161 gattataaat acaataagtg caacaagtgc tataatgatt atgaggaaat gacacatgtt
     5221 catgaatatt cagaaagtgt taagttagca gaagaatgtg aggatagaca taaccaccgt
     5281 gcagcagggg ttactggtga agctatacca ataaatggag gtacaaatca tgttcataaa
     5341 ataaatgata atgtagattt ccttgaccat ttccataaga tatgtgttac aacagggcca
     5401 gcaattagaa tacctggaac tgataaacac attcacttaa tatgtggaga aactactgtt
     5461 aatgatggtc attgtcataa attccttttt actactcaaa tagaagcacc tttagtataa
     5521 actgatatgg attattgtag taaatttgct agattatagt atataataaa tgttaaaaat
     5581 atataataga taatatttaa acttaattag gactatgata cttaaccttc gtagtccttt
     5641 ctaagttttg tataaaaaag tgttatacaa ttaaaagtga tatagggagg aaattttatg
     5701 aaatctggaa taattcttga aatgaaacaa ataaattcaa cagaatatga cgaaatgttg
     5761 caagtctggg aatcatcagt aagagcaacg catgattttt taacagaaaa agatattgaa
     5821 tctttgatac cattagttga aattggactt aaagaagtag aaaatatagt ttgtataaaa
     5881 gataatgata taataaaggg ctttatagga atagataaag ataaaattga gatgctcttt
     5941 atagaagata aatatagagg aaatggtatt ggtaaaaaat taataaaata tgctatagat
     6001 aaatacaatg taaaatatgt tgatgtaaat gagcagaata aaaaagctgt aggattttat
     6061 attcacttag gatttaaagt gtttgatagg tctgaaatag atggacaagg taacccattt
     6121 ccaatactac atatgaaatt aatttaaagt tatacttctc acttattcgc aaaaatattt
     6181 acttaaattt ttatacaaat aaaagtcgtg acagtcttag gacacgactt ttttactaca
     6241 ctattaaatt gaaatttatc tgttctatat tatgattatt agatgtagtt tttttatttt
     6301 tactatgtac ttaaatacca agtataccca tacctatcaa gtacttcagc agcgccatca
     6361 ttttgatgtg gtggtgggtg tggtaaaaat tcttgtacaa gtgtaccacc ctcggcaagt
     6421 tttacagata tttcttctat cgcttctcga gatggtagaa caacacaaat cgccatgcca
     6481 gaaattacaa caggcttgtc tgtatccgca cccattatac aaccttcgta ttttgtgaaa
     6541 gtaacacttg cgtgcataat aggattgttt ggattgctgt ctaatcgcgc aaatatagtt
     6601 tttcctcctc cgaaagcctc agcataaaaa ttaaatgctt tttcacaagt accattaaat
     6661 attaaatatg gagttagcat gaccaatata tcctccttga tatagaataa tttatagttg
     6721 cacctgataa tctaaattat gatttagtaa attgtatcat agggaaacct tgaagtatat
     6781 aattttttat tattatatta tacaaattta ttaatttaaa aaaatgttat atgtacggaa
     6841 ggtttacagg ataaaatata ataaaaagat taaggtgaca ttataaaaaa tgtatattga
     6901 cattgacgct acgtaattgt atatactttg tcttaaagga gatggtaaat atgaatatta
     6961 tagaaattcg ctcagataaa atatacaaga agataatgga tgcaccaata aacaaaaaag
     7021 aagatatata cagatatgaa ttgatgaagc cttttgaatt taagtggaag tgtatgaatg
     7081 ttccaatagt tgctagacag aaaggtggat atgatgtaat tatagcaagt gaaatgttag
     7141 gggttttatc gcctaaggat attgatgaaa agcaaaaaaa gaatataaat gtgttatctg
     7201 ctgataaaat ttggggcact tgcaaagaaa ccatagaaaa ctctataaat gcttttataa
     7261 aagaagggta tgatttaaac attaaggact ataaatattc aatattattg gcgaatccaa
     7321 atagtcctta tacaatatta agtgatggat actggggtga tggtgggatt cctggatata
     7381 tatttctatc attggttcct aatgaatata ctatcaatag attaccagta ttaatagcac
     7441 atgaatgtaa tcacaatatt agatttcagt ttatagagtg gaataataat ataacattag
     7501 aagaaatgat gataaatgaa ggtcttgcag aaaattttgc aacatggatg tttggagagg
     7561 aaatgttagg accttgggtc agtagaacag atatcgaaac attaaatact tatataaagc
     7621 caataataaa aagtgcttta aaagaaactg gatttcaaaa tataacatct tatctttatg
     7681 gtgatgatat agctaaaatg caaggatatt ttccagtagg gttgccttat tgtgcaggat
     7741 atgcttgtgg atattatatg attaagtatt atttagaaaa gacaaataaa tcaataatcg
     7801 aagcgacttt attg
//
LOCUS       DAEPOZ010000173         7793 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.173, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000173 DAEPOZ010000000
VERSION     DAEPOZ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7793
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.173"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            104..220
                     /gene="rrf"
                     /locus_tag="KR440_002802"
     rRNA            104..220
                     /gene="rrf"
                     /locus_tag="KR440_002802"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            347..856
                     /locus_tag="KR440_002803"
     CDS             347..856
                     /locus_tag="KR440_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBH0699819.1"
                     /translation="MNSDERRSKLIDILKESKCPVKGGMLAEILNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHRNSKEIYDELKIIVDLGGIVKDVIVNH
                     PTYGQITAELNIHSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIETLKEETIEVITK
                     ELEDRGILS"
     gene            868..1749
                     /locus_tag="KR440_002804"
     CDS             868..1749
                     /locus_tag="KR440_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBH0699820.1"
                     /translation="MNFLSLAKAASTNSNITKRTLENMLNNFSDNIPNIIYAIIVFVI
                     GIYISKIVRKMVSRFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGVIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFINEKR
                     ILDDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENNIKVPFVP
                     SSLLFNNMSNSNTMDKR"
     misc_binding    1876..2082
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2207..3478
                     /gene="serS"
                     /locus_tag="KR440_002805"
     CDS             2207..3478
                     /gene="serS"
                     /locus_tag="KR440_002805"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBH0699821.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKTRLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEYKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     gene            3798..4253
                     /locus_tag="KR440_002806"
     CDS             3798..4253
                     /locus_tag="KR440_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HBH0699822.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            4254..4345
                     /locus_tag="KR440_002807"
     tRNA            4254..4345
                     /locus_tag="KR440_002807"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4288..4290,aa:Ser,seq:gga)
     gene            4383..4647
                     /gene="ffs"
                     /locus_tag="KR440_002808"
     ncRNA           4383..4647
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="KR440_002808"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            4724..6361
                     /gene="dnaX"
                     /locus_tag="KR440_002809"
     CDS             4724..6361
                     /gene="dnaX"
                     /locus_tag="KR440_002809"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBH0699823.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKYSIDENESK"
     gene            6420..6770
                     /locus_tag="KR440_002810"
     CDS             6420..6770
                     /locus_tag="KR440_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBH0699824.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            6783..7382
                     /gene="recR"
                     /locus_tag="KR440_002811"
     CDS             6783..7382
                     /gene="recR"
                     /locus_tag="KR440_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBH0699825.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            7523..7744
                     /locus_tag="KR440_002812"
     CDS             7523..7744
                     /locus_tag="KR440_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699826.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
BASE COUNT         3159 a          800 c         1443 g         2391 t
ORIGIN      
        1 tccattcaat aaaatatata tgtagttttt agagtgttaa ctctaaagaa actagtatta
       61 ctagattcta aaaacttaat agaaatatta agcataaaga ttatgtggtt acaatagcag
      121 agaggataca cctgttccca ttccgaacac agaagttaag ctctctagcg ctgatggtac
      181 ttggtgggaa actgcctggg agagtaggac gtagccacgt aatctttttt atttgcaata
      241 aagtttaata ttaaatagaa tataaaatca atattgattt ataataagat aaaatataga
      301 attatatagt ataggtaaag tttttgattt agaaagaagg tgtaatatga attctgatga
      361 aagaagaagc aaattaatag atattttaaa agaatctaag tgccctgtaa aaggtggaat
      421 gctagctgag atattaaatg taagtaggca agtaatagta caagatatag cccttataag
      481 agctagagga tttgaaataa tagctacacc tcaaggatat ataatatata atcaaccata
      541 ttttacaaag caaataaaat gtaagaatca caggaattct aaagaaattt atgatgaatt
      601 aaagattatt gtagatttag gtggaattgt taaggatgtc atcgtaaatc atccaacata
      661 tggtcagata actgcagagc ttaatattca ctctaaaatg gatatagata attttatgaa
      721 gaaggttgag acaaatgaat ttaaacagtt atcagttcta actgaatgta gtcatattca
      781 tacaattgag acattaaaag aagagactat agaagttata acaaaagaat tagaggacag
      841 aggaatatta tcataaggag gagttaaatg aattttttat cattagctaa ggctgcttca
      901 acaaatagta atataactaa aagaacttta gaaaatatgc ttaataactt tagtgataat
      961 ataccaaata ttatatatgc aataatcgta tttgtaatag gtatctatat atctaagata
     1021 gtaagaaaga tggtttcaag gtttttaatc aaatatggaa tgagtaaagg tgtaaataat
     1081 ttcattgtat atggaatata catatctatg ttatctatta tatcattgat aagcttgggt
     1141 gttataggga tacaaactac atcagttgta gctgtattag gagctgctgg atttagtata
     1201 ggtttagcat ttaaagaaat attatcaaat ttaggttcag gtatgattat tttatttttt
     1261 aagccattta atataggtga ttatattcaa ggctcaggtg tagaaggcac agtttctgat
     1321 atacaaatat ttagtactgt tcttaaaacc ccagataata aaaccataat tataccaaac
     1381 tttcaattga caagtaacaa tataattaat tacacacatc aaaataaaag aaggatagat
     1441 ttttcatata atatttcata tgatagtgat attgatgtag ttaagtccat attgaatgaa
     1501 atttttataa atgaaaaaag gatattggat gacccaaaac caattatagg tttaaactct
     1561 ataggaaaca acactatgca aatagttgct agaccatggg ttaaaactga tgattactgg
     1621 gatgtttact ttgatgtaat ggaaaaagtc aaaaataaat ttgacgaaaa taatataaaa
     1681 gttccatttg ttccttcctc gttattattt aataatatga gcaattcaaa tacaatggat
     1741 aaaagataga atttcaatta agtaaaaaag tgaatttaat ataaaaaatt aaagaaaatt
     1801 ctaaaaaaat ttgaataata gtattgaaag atgttaatat atataatata atgattatca
     1861 aataaaacaa ttagttaaaa agcggtgaag gaaaatagta aaaaacagat tgtattcaga
     1921 gaatgagtgg atggtgagaa ctcattacaa tggtttttga atctactcta gagcttctag
     1981 ttaaagctag acggtttacc tacgttatag gtctgagagg attatacgtt ttagtataat
     2041 caattagggt ggcaacgcgg ataaaagatt ttcgtccctt ttatagggta aacggaggtc
     2101 tttttttatt tttcaaattt gtttaaatta aaagtttaca gctagaatac ttgtaaatta
     2161 attggaatta taatttatat tatcaaaatt attaggagga attaatatgt tagatattaa
     2221 aagaataaga gaaaatctag atgatatcaa aaaagctatg gaaagaagag gtgaaagaga
     2281 atttgacctt gatgctgtag tggaactaga taataaaaga agagaaatac ttcaagaagt
     2341 tgaagttatg aaaaatgagt taaatgtaaa ttctaaaaaa atacctcaat taataaaaga
     2401 aggaaaagat gtaacagaag aaaagacaag attgaaagag ttatcagata agataaaagg
     2461 tattgatgag aaagtaaaag aagtagaagc aaaaatggaa tatacattaa tgagaatacc
     2521 aaatgttcct catccagagg taccacaagg tgaaacagat gaagacaatg ttcaaataag
     2581 aacttgggga gaacctacta aatttgattt tgagtataaa gcgcattggg atatagggac
     2641 aggtctaggt attttagatt ttgaaagagc agggaaaata actgggtcaa ggtttacttt
     2701 atatagggga ttaggagcta gattagaaag atctttaatg aatttctttt taaatactca
     2761 tacagctaag catggctata cagaagtctt acctccattt atggccaata gaaatagttt
     2821 tatagggact ggtcaacttc ctaaatttga agaagatatg tttaaaatag aaggattaga
     2881 gtatttctta ataccaacag ctgaagttcc tgttactaat atacatgcta atgaaatatt
     2941 agatgttgca gagcttccaa ttaaatactg tgcatatact ccttgtttta gatctgaagc
     3001 aggttcagca ggaagagata caagaggttt agttagacaa catcaattta ataaagttga
     3061 attagttaaa tttgttaaac cagaagattc ttataatgaa ttagaatcat taactcatga
     3121 tgctgaaact atgttacaaa tgttaggtct tccatataga gttgtaaaaa tatgtacagg
     3181 agatttggga tttacagctg catttaaata tgatttagaa gtttggatgc caagttacaa
     3241 tagatatgta gaaatatctt cttgttcaaa ttttgaagat ttccaagcaa gaagagcagg
     3301 aataagattt aaaagagata aaaaatcaaa agcagaatat gttcatacat taaatggttc
     3361 tggattagcg ataggaagat gtttggctgc tatattagaa aattatcagc aggcagatgg
     3421 ttctgtagta gttccagaag aattaagacc ttatatgggt gttgatgtta taaaataaat
     3481 ttatattaat tgtatatgta gagtatgctt tgatgaagag aatatattat gagtaaacaa
     3541 taaataaaaa aactatgaaa ttattataaa tattaatttc atagtttttt tatgttaatt
     3601 tacacagcag aattttcatc ataaaacact aaatattttt ataaaacact cttgtatgaa
     3661 tatctaaaaa ttattttaga actaattaaa aaaaatatcg ctatatatag taataattaa
     3721 ataaacaaaa taaaattaag ttgaattata aaatgaatta tgataagata agtttgttat
     3781 aagttttggg aggaattatg gaaagttcat tttatatgaa agaagcatta aaagaagcat
     3841 ataaagcata taataaaaaa gaaacaccaa taggtgctat tatagttaaa gataatcaaa
     3901 taatagctag agctcataac cttacagaaa cgttaaaaga ctctacggca catgcagaaa
     3961 ttttagcgat aaaacaagca tcagaaaaat taggtggttg gaggttgact gattgtgatt
     4021 tatatgttac aatggaacct tgcataatgt gtagtggcgc aatagttaat tctagaataa
     4081 aaaagcttat aatagggaca agacatgtaa aaaattcata tatagaaaaa cagcatgagt
     4141 ttaaattaga ttattttaat aataataatg taaaagtagc ttttgatgtt ttgcaagaag
     4201 aatgctcgat catattgcaa gaatttttta aggcattgag aaaaagagat taaggagaga
     4261 tggtcgagtg gtcgaaggcg ctcgcctgga aagcgagtat atgggtaaaa ctgtatcgtg
     4321 ggttcaaatc ccactctctc cgccagaaac aatgaaagta attaaatatg gttgtaattt
     4381 tgctgtacta gatggggagg tagcggtgcc ctgtaacctg caatccgcta tagcaggatt
     4441 gaattccatc taaaggctta ttttctgtag ggccgcccta aataagtggt gttgatgctt
     4501 aggtcctgcg caatgaaagc tcatgaaccc cgtcagatcc ggaaggaagc agcggtaagt
     4561 gatcactttt gtgtgccgtg ggggtgccta agttgagcta actgttttgg taacgcctat
     4621 ggaattaagt cgatagatgg tgtacagcat ttaatatata aaagactaat cccttagata
     4681 atgagggatt tttttattaa ttttgctaaa gagaggtaaa aagatgcata aagcattata
     4741 tagagcatat agaccacaaa aatttgaaga tgttattggt caagatcata ttattaagac
     4801 tttaaaaaat caaatatata gtgataatat aggacatgct tatttgtttt gtggaacaag
     4861 aggtactggt aaaacatcta ctgcaaagat tttttcaaga gcagtaaact gtttgaataa
     4921 aataaatgaa gaaccgtgta atgagtgtga gatatgtgaa tctgtattaa aagataatac
     4981 aatggatgtt gtagaaatag atgcagcttc aaataatagt gtagatgata taagagagct
     5041 tagagaaagt gtgaaatatt ctccagccaa tgctaaatat aaagtatata taatagatga
     5101 agttcatatg ttgtctcaag gagcttttaa tgcattatta aagaccttag aggaacctcc
     5161 atcatatgta atttttatat tagcaactac agagcctcac aaaatacctg caaccatttt
     5221 atcaagatgc caaagatacg attttaaaag agttacagtt aaagatatga cccttagaat
     5281 gaaaaagatt tgtgaagatg aaggaattga tatagatgat aaagctctta atttaatagc
     5341 tagaaattct caaggagctc ttagagatgc acttagtata ttagaccaat gtatgtcttt
     5401 tggagaaagt aagatagatt ataaagatgt tgttgaatta atgggaagcg taaatataga
     5461 acaattattt gaattgtctc aatgtattgt agagcaggac actaaaaagt ctcttgagat
     5521 attaaatgaa tttgtcttat ggggcaaaga tataagaaat cttataaatg acttaattga
     5581 tcattttaga aatttaatgg tatgtaaggt ttcatcagaa ttggatgaaa taatttcatt
     5641 accagaagaa actatagaac aacttaaaat tcagtctaaa aatatagata taaatgattt
     5701 aataaggatt ttaaatatat tatcaataac tcaggatgat ataaagtcat cttcaaatcc
     5761 aagggtttta gtggaaataa ccataatgaa aattgctcaa ccaatgtttg atgaaagtaa
     5821 agaagctctt ataaaaagag ttgagaactt agaaaaaatg attgaactgg ggaactttaa
     5881 gtctgaaaaa attgggaata ataaagaaaa agagtatgag gtcgatagag aaatagatgt
     5941 aaaacaagaa aatgtagttt atgaagatgt aaaaaatgag gacgttatac ttatagagtc
     6001 atcttggaag aatatactta aacaaattaa aaaagataaa aaaatgccaa tatacgcact
     6061 cttaagtgaa gtaaaaagtt ttaatgtata ttctaatatg ttgtatgtta tatttgatga
     6121 taaatttgat tttgcaaaaa ctagactgag ctctcaagat acaataaatt atttagaaaa
     6181 aacaataaga gatgttttaa atagaagttt taatgtaaaa atagtactga catcagaagt
     6241 taaagatata aatcttgaag ttaaagagaa aaaggacatt ggtgaggaga tattaaagaa
     6301 catagtgagt gaagagatat tagaaataaa atatagtatt gatgaaaatg agagtaaata
     6361 ataaaatact attatgatat aataataaaa tgaaaataag tttaggagga atagtgttta
     6421 tggctaaaaa aggttttgga ggcggaatga tgcctggtgg tggaaatatg aacaacttgt
     6481 taaagcaggc tcaaaaaatg caagaaaata tgcaaaaagc tcaacaagag ttagaaagta
     6541 aagaagtaga agcttcagtt ggtggaggag cagttactgt aaaagttaat ggtaaaaaag
     6601 aagttataga tataactatc aagccagaag tagttgatcc agatgatata gaaatgctac
     6661 aagatttagt tttaagtgca gtaaatcaag cattgagaaa tattgatgat atacaagcaa
     6721 gtcaaatgag taaggtaact ggaggtatga atatacctgg gttattctag taggtggtat
     6781 ttatgcaagt ttatacaggt cccataacga gacttataga ggaattttca aagcttcctg
     6841 gagtaggaag aaagactgct caaagattgg cttttcatat tattaatatg aatactaatg
     6901 atgtggaagc tttatctaaa gcgattatag atgcaaaaag agaaataaga tattgctcaa
     6961 tttgttgtaa tataacagat acagacccat gtagcatgtg ttcaaataaa agtagagatt
     7021 caagtgttat atgtgttgtg gaagatccaa gagatgtagc agctatggag agaacgagag
     7081 agtttaaagg acagtatcat gtattaaatg gagtaatttc tccaatggat ggaattggac
     7141 cagatatgtt aaaaataaag gaacttatcc aaagacttgg caatcaggaa gtcaaagaaa
     7201 taataatggc aacaaatcct actattgaag gtgaagcgac agccatgtat attgctaggt
     7261 tagtaaaacc aatgggaatc aaagtcactc ggatagctca tggtcttcct gttggaggcg
     7321 atttagagta tgcagatgag gtcacaatat caaaagcact agaaggcaga agagaaatat
     7381 aaaaatcgag gttaatacct cgatttttgt agttttttga gttaaattca aattaatttt
     7441 gatttagatt tattgaataa atttcttctg tattgtataa tttgttgtat aaatcattta
     7501 aaaattttga taggggtgca acatgaaacc aattatgaac ttagaagaac ataatgcaat
     7561 gaaagaaaaa agagttaaaa aaatgaaatc aaaagataaa cctaaatatg atgattttga
     7621 ggatataagt ttttctaata agaaaaaaac taaaaaaaat gtacgaaata aaaatgctga
     7681 atttagaaat aattataaaa attatcagta tgattatgaa gaagactttt atgaaccaat
     7741 ttaatattaa ataattagaa aataaaaaat ccctaataga taatatatat act
//
LOCUS       DAEPOZ010000174         7759 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.174, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000174 DAEPOZ010000000
VERSION     DAEPOZ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7759
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.174"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..959
                     /locus_tag="KR440_002813"
     CDS             <1..959
                     /locus_tag="KR440_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBH0699827.1"
                     /translation="SGYVFITKESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDI
                     INDICREQNIKEIGFEGNEVSFDLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKK
                     ACEIVDATFYHIVDFIKIGMTEKQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHG
                     KASEKIIEYGDFVTFDFGAKYNNYCSDITRTICMGTINKELEEIYNIVRKANEECIRV
                     LRPGMTTGEIDKVARDIIGSYGYANNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMI
                     VTIEPGIYVPSLGGVRIEDDVLITQDGCMRLTTSTKDLIVVS"
     gene            1013..2191
                     /locus_tag="KR440_002814"
     CDS             1013..2191
                     /locus_tag="KR440_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HBH0699828.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFVPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGKFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKNIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
     gene            complement(2382..3440)
                     /locus_tag="KR440_002815"
     CDS             complement(2382..3440)
                     /locus_tag="KR440_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBH0699829.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTIFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSEIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLEDGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKDGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMDLINEIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            3507..3878
                     /locus_tag="KR440_002816"
     CDS             3507..3878
                     /locus_tag="KR440_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBH0699830.1"
                     /translation="MKKKDILLILGILVVIIACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIENRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            3915..4589
                     /locus_tag="KR440_002817"
     CDS             3915..4589
                     /locus_tag="KR440_002817"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBH0699831.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            4838..5677
                     /locus_tag="KR440_002818"
     CDS             4838..5677
                     /locus_tag="KR440_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBH0699832.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDANDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            5920..6016
                     /locus_tag="KR440_002819"
     tRNA            5920..6016
                     /locus_tag="KR440_002819"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5954..5956,aa:Sec,seq:tca)
     gene            6167..6874
                     /locus_tag="KR440_002820"
     CDS             6167..6874
                     /locus_tag="KR440_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HBH0699833.1"
                     /translation="MENNFTTRTSFLVGDDGIEKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(7016..>7759)
                     /locus_tag="KR440_002821"
     CDS             complement(7016..>7759)
                     /locus_tag="KR440_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0699834.1"
                     /translation="VGQGDSILVQTPTNKNILIDGGDEDSENIIISYLRQKRIKTIDI
                     IIATHPDSDHIGSLDNVIKKFNVNSIYMPEQSTDSEAYQNLINSCTDKNLSIQHLYKN
                     DVLNIDNNINIYVLSPSYIQEESNLNSIVFKLTFNDNSFLFMGDAEEENEKEILHSFK
                     LNNINFIKIGHHGSNSSSSLEFIKKISPDIAAISCGYKNQYGHPHREVINNLKQNHVS
                     IYRTDRIGDIVFYSDGEIIFTKYNYEIDT"
BASE COUNT         2999 a          829 c         1276 g         2655 t
ORIGIN      
        1 cttcaggtta tgtttttata acgaaagaat cacaatatat aatagtagat tttagatatt
       61 ttgaagaaat taaaagaaaa tctagtttgt ttaatgtagt attaatggac aaaactagaa
      121 ctcattttga tataattaat gatatttgta gagagcagaa tataaaggaa attggatttg
      181 agggaaatga agtatctttt gatttatata gaagtatgag taataagtta agtgcaacct
      241 taaaatcagt tgatttatct acattaaggg aaacaaaaaa tgaagacgaa ataaaatata
      301 taaaaaaagc ttgtgaaata gttgatgcta cattttatca tatagtagac tttataaaga
      361 ttggtatgac tgaaaagcaa gttgaaaatg aaattgttag aattataaaa gatttaggtg
      421 gtcaaaaaga atctttcgat acaatagttg catctgggct tagaggagca ttacctcatg
      481 gaaaagcaag tgaaaaaatt atagagtatg gagattttgt tacttttgac tttggtgcta
      541 agtataataa ttattgttca gatattacca gaaccatttg tatgggtact ataaataaag
      601 agttagaaga aatatataat atagtaagaa aagctaatga agagtgtata agagttttaa
      661 gaccgggaat gactactgga gagatagata aagttgcaag agacataatt ggttcatatg
      721 gatatgccaa taattttggt cataatctgg gtcatggtgt aggaattatg gttcatgaat
      781 atcctgcctt agctcctgaa tcaaatgaag tattaaaaga aggaatgatt gtaactatag
      841 agcctggtat atatgttcca agtcttggag gcgtaagaat agaagatgat gtactcataa
      901 cacaagatgg atgtatgaga ttgactacat ctactaagga tttaatagtt gtaagctaaa
      961 aataaattag ataaatattt tataacaata agaaaagaag aggtaaattt aaatgtataa
     1021 ttttgatgat aaaccagata gagtaagtga aaagtgtaga aaatgggatt taaatattat
     1081 aagagataaa tttggagata ttagagaaga ttttgtacca atgtggattg ctgatatgga
     1141 ttttaaaata ccaacagaaa tagaaaacaa atttattgaa gctgtaagaa gaggtgtatt
     1201 tggatataca tactgttatg atgaatttta tgattcagtt attggttggc aaaaaaatat
     1261 gcatgaggta aatgtagaaa aagaatggat tacattaact tatggaactg tatcaacact
     1321 tcattatgta gtacaagctt tttgtaagaa aggtgatagt ataatattaa acacaccagt
     1381 ttatgacccc tttgaatcat cagcaaaaaa acaaggtgta aatgtaattt gtaatacatt
     1441 agatgttgta aatgatagat attatataaa ttttgataaa ttagaagttc aaattaaaga
     1501 aaataagcct aaattaatga tgttttgtac accacataat ccatctggaa ggatatggac
     1561 aattgaagaa atgacaaggg tagcaactat atgtaaagaa aataatgtta ttttggttgc
     1621 agatgaagta catgcagagc atattcatta tggtaaattt aattcaatac ttaagattgg
     1681 aaaagagctt ttagaaaatg ttattctact gacatcacca aataagggct ttaatcttgg
     1741 tggattaaag acttcttatt caatagttat aaataagaat ataagggata aatttagaaa
     1801 taaattaaag caaaattcta taacttctcc aaatgtgttt ggaataatag gattgataac
     1861 cgcttataat gaatgtcatg agtggttaag tggtgtaaat gaatatataa aatcaaatta
     1921 tgaactttta gaaacttggg taaataaata taataagata aaattgatga aaatggaatc
     1981 atcttactta gcttggatgg atataagtgg attaggtata agtgcaagtg agtttacaga
     2041 taaattggct atagatacag gagtattatt agaagatggg agtcattttg taaaagatgg
     2101 tgagaagtat gttagaataa atttaggaac tcaaaaggaa aatgtaattg aagctttaaa
     2161 tagaatggat ttatttttaa agtctttata aaaatttggt tgaattagta ttttatataa
     2221 atttaagcta ttaatatggt atatacataa gtagaatttg ttttagatta cttaagtata
     2281 taccatattt tatataaaaa ttaaatttgc attagtcatg tgataactat ttaaattcat
     2341 atgtattttg tatgtataat agatataatt attatttaaa cttattgttt atttaatttt
     2401 tcaatagaaa aagatttatc tgtaagttta aaattatctt ttataccctt agttaaatat
     2461 actttcttat ccttagttat gaatatagca tctacattat caatttcatt aattaaatcc
     2521 ataccttttt caagaccaag tccaaatgta gaagtggata aggcatcaca attaatagat
     2581 ttatcagaaa ttattgtaac actacttaga ttgttttcaa aaggataccc tgtaaatgga
     2641 tttaacatat gatgatatat ttttccgtct ttttcgataa atctttcata aattccagaa
     2701 gtaacaacag atttatttgt ggtttctatg tttcctatgg attcaccatt tttactagtg
     2761 gggtcttgaa tgcctatttt aaaaggttta tcatttttac catcttctaa agtaaatata
     2821 tttcctccta agtttactaa accttttttt acgtcctctg attttaaata ggctacaatt
     2881 ttatcagcag catatccttt agctatggca cctaaatcaa ttttcatatt ttctttggtt
     2941 aattttatag atttattttt ttcatccaat atgatgttct tataatcaat aagaggcaga
     3001 agtttttgta tctcagaatc acttggaact ttagctttat ctgttccaat agcccataaa
     3061 tttgataatg gaccaattgt tatgtcaaaa tatccatctg ataatttgga atattgtata
     3121 gcttctttta cgacgaaaaa tgtatcatca gaaactctga caaatttttt accagcatta
     3181 tcatttattt tagaaacatc acttcctggt atatgagtgc tcattttgtt gtcaatatat
     3241 cttaaaattg aatcacattc atttaagatt ttatcacttt tggattcttt tacattaaat
     3301 atagtaactt cgtttactgt accaaggtag tagctagttt tactatattt agtattctgg
     3361 cttgaactcc taaaaaatga aaataaaatt aatgctaaaa tcaatatcac aaaaataaat
     3421 gtgatttttt tcttattcat ggtatattcc tcctatgaaa ttgtgttatt attttattat
     3481 aacataattt cataggagga caaaaaatga agaaaaaaga cattcttctt attttaggca
     3541 ttttggttgt tattatagca tgttatggga taataaatgt tataaattct aaaaatgctg
     3601 gaaatattga gatttatgta gataataaat tgtataaaac tgtttcaata aatgccaaag
     3661 aagaatttaa aatagagaat agaggggggt ataacatagt aaagattcat gataaagggg
     3721 ttgagatagt agatgcttca tgccctgata aggtttgcgt acatactgga tttataaaca
     3781 agccaagtca aagcatagtt tgtataccta atagagtgag cataaaaata aatacaaatg
     3841 aaaagaatga taatcaagaa gatataattt ctaaataaaa taataaaaat aattgaaagg
     3901 aattggtgat aattatggtt aacttaggaa acgattggga tgaattattg aaagaagaat
     3961 ttgaaaaaga ttactactta aacttaagaa aatttcttat agaagaatat aaaacacgcc
     4021 aaattttccc aaatatgcac aatatatatg aagctttaaa acacacttca tacaaagaca
     4081 caaaagtctt aatattagga caagaccctt atcatggaga taatcaagct catggtttag
     4141 ctttttcagt tcaaccacaa gtgaaaactc caccatctct attaaatatg tataaagaat
     4201 taaaagatga tttaggttgt tttataccta ataatggata tttaatgcct tgggcagacc
     4261 aaggagtgtt acttttaaat actgctctta ctgttagagc gcatgaagct aattctcata
     4321 agaacaaagg atgggaaatt ttcactgata gagtaatatc tattttaagt gagcgtgaag
     4381 acccagttat ttttgtacta tggggaagta atgcacgtaa aaaagtagag ttgattgata
     4441 caagtaaaca ttatattttg gaagcacctc atccaagccc attgtcagca agtaaaggct
     4501 tttttggatg taaacacttc tctaagataa atgaaatttt gaaaaagcta ggaaaagaac
     4561 ctattaattg gcagatagaa aatatataaa agtaaaaggt aacaaaatga taacaaaaca
     4621 acaataaaca atgacattgt tgtaactaat agaaatatgc ttaaatagtt gaaaatacta
     4681 tgtatgcata tttctatttt tttgtcaagt tgacaactct tgaaaaaata gttacaattt
     4741 agttacacaa aaaacacaaa taaaaaaaga aacttacaaa gcagaatttt atgaagcaaa
     4801 atatatagtg taaaaaattt tagggaggaa attttatatg aattttaaaa aattgagtaa
     4861 tgctaaagtc tcggtaatgg cagttgtttt atcagttgga atattatcgg gatactctgc
     4921 gcttaataaa caagtaacat tagtagtaag gggagaagaa agaactatat caacatttag
     4981 ttctaatgta ggagaccttt tagaatctca aaacattagc tatgatgcaa atgatatagt
     5041 aactttagat gaaaatacaa agttatctaa tggagataaa atagaagtaa ttgatgtaaa
     5101 ggaaaaaaca gttgtagaaa ctaaagacat tccatttgaa gttacagttg ttgaagatgg
     5161 ctctttatta aagggtgatt ctaaggttaa agaagaagga aagtctggta aaagtgaatt
     5221 aatatataaa gaaacttatc acaatggaaa gaaagttgaa aaggaatttg tgaaaaaggt
     5281 tgttaaagct gagccagtaa ataaggttgt aagcaaaggg acaaaggtag aaatgcaagt
     5341 agcctcatca aggggagaaa gttcaagaag aatggcttca tcatcatcta gtagcagtag
     5401 tagtaacaat ggtaaaaata tgagagtggt agcaactgca tatactggtc atagtataac
     5461 tgcaacagga acaaaaccta agtggggaac aatagctgta gaccctaagg ttataccata
     5521 tggaactaaa gtttatatac ctcaatttga catggtgttt actgcagaag actgtggtgg
     5581 agcaataaaa ggaaataaaa ttgatatcta catgaatgat tcaaaaacgg tttataactg
     5641 gggaagaaaa actatagata tacgtatatt aaaatagtta aataatttat atttttactg
     5701 gaaattatat tttgattata tagagaagaa actctttata gattcttaaa atataatttc
     5761 cagtttttat tttggatatg taataaaaca ggtaaatttt aaccatgtat agataaaagc
     5821 tatttaaatt ataatttact atcagataaa tctataaaaa ttgttgcaaa gagtcaaaat
     5881 atatgatatt atagatttcg ttgagaagga tttcattatg ggagtggatg ggtgccggtg
     5941 tgccctctag tcttcaaaac tagtatgagg ggttaagagc ctcttaggtg ggttcgattc
     6001 ccacgcattc ccgccaatat gcaaaaggta tcttttaatt aaaagatacc tttttaaatt
     6061 tattttaaga taatttattt ttaataatat ataatgtttt gtattatata atgtaaattt
     6121 tctaatacta ttatatattt agataaaaat ctaggagtgt ggaaagatgg aaaataattt
     6181 tacaacgaga actagttttc tagttggaga tgatgggata gaaaaactta ataattcaaa
     6241 tataatagta tttggagttg gaggagttgg tagttttact gttgaagctt tagcaagagc
     6301 tggagtaggc aatattacta ttgtagattt tgatgatgta gatataacca atataaatag
     6361 acaaatacca gccctacatt caactgtagg aaggtacaaa gttgatgtca tggaagaaag
     6421 aatattagat ataaatccta atatcaatat aaaaaaaata agaagtcttt ataataaaga
     6481 tactagtgac gaaatactaa ctgaaagata tgattatgtt gtggatgcga tagacatggt
     6541 aagttccaaa atacatctaa ttgaaacttg tgaaaaaaaa gggttgaaaa taataagttc
     6601 tatgggtatg ggaaataaat tagacccaac taaaatagtc gttacagata tacacaaaac
     6661 aagtacttgt cctctagcta aagttatgag gaaagaactt agggatagag gtatcaaaaa
     6721 gttaaaagta gtatattcta ctgaacaacc aatagaactt aagaagaaag taatgaatgg
     6781 tagaaaagta acacctggta gtgtatcatt tgtcccatct gtaggaggtt taataatcgc
     6841 atctgtaata gtaaatgaac tattaggaca ataagtttta aatattttaa tttataaatt
     6901 ttatatacaa atttgaaggc tagaagcatg aatataaagc aattttatat tcatactttt
     6961 agcctaatta ttttatatta ccttatttta tgtagttgta gttttatttt ttagattatg
     7021 tatctatttc ataattatat tttgtaaata taatttctcc atcactataa aaaacaatat
     7081 ctcctattct atctgttcta taaattgata catggttttg ttttagatta ttaataacct
     7141 ccctatgtgg atgaccatat tgatttttat atccacatga tattgcagct atatctggac
     7201 taattttttt tataaattct aaagatgaag aagaattact tccatgatga cctattttta
     7261 taaaattaat attatttaat ttaaaagaat gtaaaatttc tttttcattt tcttcttcag
     7321 catcacccat aaataaaaaa gaattatcgt taaaagttaa tttgaaaact atagaattta
     7381 aattagattc ttcttgtata tatgatggac ttaatacata tatatttata ttgttatcta
     7441 tgtttaaaac atcattttta tagagatgct gaatagataa gtttttatcc gtacaagaat
     7501 ttattagatt ttgataagcc tcactatcag ttgattgttc aggcatatat atggagttta
     7561 cattaaattt ttttataaca ttatcaagac ttcctatgtg gtctgaatct ggatgagttg
     7621 ctattataat atcaatagtt tttattcttt tttgtctaag ataactaata attatatttt
     7681 cgctatcttc atcacctcca tcaattaata tattcttatt tgtgggtgtt tgaactaaaa
     7741 tactatcacc ttgtccaac
//
LOCUS       DAEPOZ010000175         7714 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.175, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000175 DAEPOZ010000000
VERSION     DAEPOZ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7714
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.175"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..552
                     /locus_tag="KR440_002822"
     CDS             <1..552
                     /locus_tag="KR440_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBH0699835.1"
                     /translation="KIREKIKKLRKISSKMQNRINITVEGISKMNSEEIYISKKFEEY
                     LVLSSDLSNMSNSDFMMELIDFTNYCKSKNLYQGYELGVIVSNENIKNGDYLSISSFY
                     LKIDKKIKDKKLYVKPEGMYACIKHIGKYEDSYKSYEKLKKYIYENEYKIIGNSYEES
                     ILDFFCESNEDNYMTEISIQVSR"
     gene            complement(685..2805)
                     /gene="feoB"
                     /locus_tag="KR440_002823"
     CDS             complement(685..2805)
                     /gene="feoB"
                     /locus_tag="KR440_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBH0699836.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(2831..3058)
                     /locus_tag="KR440_002824"
     CDS             complement(2831..3058)
                     /locus_tag="KR440_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBH0699837.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            complement(3385..3825)
                     /locus_tag="KR440_002825"
     CDS             complement(3385..3825)
                     /locus_tag="KR440_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0699838.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKS
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     INLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            3977..4966
                     /locus_tag="KR440_002826"
     CDS             3977..4966
                     /locus_tag="KR440_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HBH0699839.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRSLNQFLCCSKKAFYICV
                     SYLAIFEVLKLLNVKVDSVYLKTIFISITYISVYFIIRDSCMSLQNFIIAKFRKLLYA
                     RILFLIIILLLFFIFDVTVISLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
     misc_binding    5118..5370
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5442..6650
                     /locus_tag="KR440_002827"
     CDS             5442..6650
                     /locus_tag="KR440_002827"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBH0699840.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     gene            complement(6711..7592)
                     /locus_tag="KR440_002828"
     CDS             complement(6711..7592)
                     /locus_tag="KR440_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBH0699841.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPGRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
BASE COUNT         2844 a         1025 c         1128 g         2717 t
ORIGIN      
        1 aagataagag aaaagataaa aaaactaaga aagataagct ctaagatgca aaatagaata
       61 aatataacag ttgaaggaat ttctaaaatg aatagtgaag aaatttatat atccaaaaag
      121 tttgaagaat atttagttct atcaagcgat ttgagtaata tgagtaatag tgattttatg
      181 atggaattaa tagatttcac caattattgt aaatcgaaaa atttgtatca aggatatgag
      241 ttgggagtaa tagtaagtaa tgaaaatata aaaaatgggg actatttaag tatatcaagt
      301 ttttatttga agatagataa aaaaataaag gataaaaagc tatatgtaaa gccagaagga
      361 atgtatgctt gcataaaaca tattggaaaa tatgaggatt catataaatc ttacgaaaaa
      421 ttgaaaaaat atatatatga aaatgaatat aaaattattg gaaattcgta tgaggagagt
      481 atacttgact ttttttgtga aagtaatgaa gataattata tgactgaaat ttcaattcaa
      541 gtatccagat aagaattaaa atagagatat attcaaatgt taagtaattt tacttatcat
      601 tgaatatatc tctatgcttt ataataaagt atataaattt ttaaatagtt tctctaatat
      661 atgtctaatg tcagttaata ctaattatat aaataaattg taaactgtag ttgttaaaaa
      721 acaaacaaca aaagcaagta tagtaggcat taagaatcct aaggcagtcc attttaaact
      781 accagtttct ttttttattg ttaaaagtgt tgttgcacaa ggccaatgca gtaaactgaa
      841 cagcataaca tttaaagctg tcaaataagt ccatccatgt tctctaagaa cttgacctaa
      901 agcactaaag ctatctaatt ctatcataga accagttgct aggtaagcca ttaaaagtat
      961 aggtacaact atctcatttg ctgggaatcc aagaataaaa gcaagtaata taaaaccatc
     1021 aagaccaatt aatttagcta aagggtctaa aaagtttgca acatgtgata atatgcttaa
     1081 atctcctata tatatatttg caaatatcca agttatgaca ccagcaggta tggctaccat
     1141 tacagccctt cctaaaacaa atattgttct atctattata gaggtatata aagttcttcc
     1201 tatttgagga actctataag gaggaagttc taatgtaaat gtagacggaa ctccttttaa
     1261 tagcgtctta gaaagagtat aagaaaccaa caatgttatt ataacaccta gaattatcag
     1321 taaagttatg caaagtgcag ttgctacgct tgatacgaaa gagttggtta taactgagct
     1381 aaaaaatatt gtagatatag ctatcaaagt aggaaaacga ccattacaag gaacaaagtt
     1441 gtttgtaagt atagctataa gtctttccct tggagagtca attatcctac aaccaataac
     1501 tccagctgca ttacatccaa atcccataca catagtcaaa cactgttttc catgtgcaca
     1561 agctttttta aataggtggt ctaagttaaa tgcaactctt ggcaaatatc caaaatcctc
     1621 aagtagggta aataatggaa aaaatattgc cataggaggt agcattacag agattaccca
     1681 tgctaatgtt ctgtataatc ctaaaaccaa catatcattt aaccatgatg gacaattaat
     1741 actatttaat atacccgaaa tacttggctc aaatccgagt aataaattag ataataaagt
     1801 ggatggatag ttagcccctt ctatagtaat ccacaatata gtacctaata ataagagcat
     1861 tattggaagc ccaaatattt tagatgttaa tattttatct actttttctt ctctatcagt
     1921 tgatttttta gcaacattag aacaacactc atcactcagt tttttagcat aatcatagtt
     1981 gattttagta aactcatctc ttattttctg tttatttata ttgtctggaa tctttttctt
     2041 tacttcatta atagcatcta tagaatcttt gtctatataa ttactcatag attcaaatat
     2101 actttcatcg ccatcaataa gtctaagtcc caaccatcta gaatttatac ctggtataat
     2161 attatccaat tctggttgaa ttgattttac cacattctct atattttcat tgtatctaac
     2221 aggtttatta tttaatttgt atttgtcaaa agatacatca tttaaagtat ccaatagttc
     2281 atccatacca gagccatttc ttgcagcagt taaaataact ggaataccaa gagaatcctc
     2341 taaaagtttt ttatcaatag taatgccttt ttttctagct tcatctatta aattaataca
     2401 caaaataact ttatctgtaa gttccatcac ttgaaatact aaatttaaat ttctttcaag
     2461 acatgtagca tcacatacta ctattactgc atctggatta ccaaaacata taaaatcacg
     2521 agctacaatt tcttcttgtg ataaggcaaa taaagaatat gttcctggta aatctattaa
     2581 tgcatattcg gtatttttat atttaaaatt accacgagct gtagctacag tttttcctgg
     2641 ccagtttcca gtatgctgtc taagccctgt aagatgatta aatacagtac ttttacctgt
     2701 atttggattg ccagccaatg caattacaaa ctggtcttcc ttattgtcta tatcaaacat
     2761 atcttttaaa gaactcattt tagttgagtt atgtgtcaac cccatattaa tatcctcctg
     2821 ctagaaattt ttaaatgtca gaaactaata ttaagctact ttcttcttgt cgtagagcta
     2881 tcttactacc tctgatttta tagacagtta aattattttt tggacctttt ctaactacat
     2941 ctataacagc tcctctagta agaccaagcg ctaacattct ttctcttaaa ttaccactag
     3001 ataatatatc ttcaactttg acagtttttt tcacttgaat atcgttaagg tttttcatag
     3061 tagcacctcc atgcttgaaa tttagcctgg gctaatcttc ttattctaaa ctatgcatta
     3121 tattattacc ttgttacttt tacaaaaaaa agataggcaa gatgcctatc atacaaagtt
     3181 gggtataatt cttacttttg gggaaagtag aatatagaat aaaaattaac actttgaatc
     3241 catataatta ttgaattttg ataataaaaa agtcagaaag caaaagcctc ctgactctat
     3301 gtaaggtatt ttcaaatacc taaatatcaa ttcaatattt aatttatact taaattatat
     3361 attaacaata ttagcttgtc aacactataa tctcatttta atatgttcaa tacctgcttc
     3421 gtcgtatact tctgagattt ctttaaaacc acaagataag taaaggttct ttatataagt
     3481 ttgtgctgat aaggttatat tattttcgtg taagttaatt tttataaaat ctatagcaca
     3541 atccatcatt tgttttgcta atccttttct tctatgacta gaaagaacta aaactctacc
     3601 aatggaaaca tcattataag agctgtattc tttaggaatt attctagcat aagcacaaat
     3661 caatccattt tcttttaaaa atatgtgata agagctttta tctatatcat cataatcgtt
     3721 taatgaaaat atcttctgtt cacaagcaaa tacctcatat cttgattttg ctatttcata
     3781 aaattcatct aaactcaaat cattaaagtg tttaatttta aacatagtaa ttacctccta
     3841 gcatcttaaa aaactgaata ttaagataca ttataccatt tttaacataa atacctttat
     3901 taaaatttat atttattaaa aatattttta aaaagtgata tactatatta aaaacaaaac
     3961 tttagttaga aaatttatga tttatggagg cagtgttttg aatactacta aaaaattaac
     4021 tgaagcagct ttattgtcat ctttattcat agtagtaaca attatagctg tgagtactgg
     4081 atttggatat gctatttact tagattttat agtgccagta tttttttgta taatctgctt
     4141 gaaatgtgat ttaaagtata ccattttatc tggaattact tcacttttaa taatttctct
     4201 ggtacttgga aatctaggaa ctgctatttg ggcaagtcag agtgtattac ttggtataat
     4261 gtgtggctat ctaataaata agcctacaat ggttatggat gatttagtat atggttcagt
     4321 atttggggtt attgtaatgg ttttcattga tatatatgct tcaacattaa taggttatag
     4381 ttttatgaaa gaatttcaag gatatagcaa atggatttat tttaatgggt atacaaattt
     4441 tatttattat ttaatgatag cattgtttcc ttttggtatg gtgttttgca tatatttatt
     4501 aagtttaatt ttagggaata aattgcatat tttagattct aattctttaa aaaaattttt
     4561 aatatttaag aattttagaa gtttaaacca atttttatgt tgctcaaaaa aagcttttta
     4621 catatgtgtt tcttatctag ccatttttga agttctaaaa ctgttgaatg ttaaagttga
     4681 ttctgtttat ttaaaaacaa tctttatatc aataacctat ataagtgtct attttatcat
     4741 tagagactca tgtatgtcac tacaaaactt tataatagct aagtttagaa aattattata
     4801 tgctagaatt ttatttttga taataatttt attgctgttt tttatatttg atgtaacagt
     4861 tattagttta ataattataa acgcattttt aaataagaaa ataaatataa gattgagtca
     4921 gagtaatata gtcaataaac aaataaatct tttgattgaa aaataaactt tgggtattgg
     4981 taaaaaatat aaaaaagtag tataatgtaa aaaaacatgt atataataat aaaaaaggtt
     5041 gacaaaatat aaaaataata atatattatg agtaagttaa gaaaaatttc ttgtaattga
     5101 tgaaataatc tgaatatata aatgctatga agagaagaag tagggctaga ttttatttaa
     5161 cagagagttc cagtttggtg aaatggaata aattgattta gtttgaatac atctcagagc
     5221 actatctctg aaagataaca accattaaga gatatcggat tagtacacgt tatagtacac
     5281 gagtatatgt aattgtactc taagagatta atatcgtgag atattgatga actaaggtgg
     5341 taacacgtaa gcaatgcttt cgtcctttta agaggatgaa agcttttttt attataaaaa
     5401 aaaaattata gattatataa atattttagg aggaatgaaa aatgaaatcg atagacgagc
     5461 aaatgcgaat aattatgaaa ggtgtagatg atttaataga tgagaaagag ttaagggaaa
     5521 agcttattaa atcagaaaaa gaaggaaagc ctatgatagt aaaattgggt ctagacccaa
     5581 gtgctccaga tatacattta gggcatacag tagttcttag aaagatgaaa caattacaag
     5641 atttaggtca tcaaatagta ataataattg gagattttac aggaaaaatt ggagacccaa
     5701 ctggaaaatc taaagctaga aaagctttaa caacagagca agttcttgca aatgcaaaaa
     5761 cttatgaaga acaaattttc aaagttttag ataaagaaaa aactattgta agatttaata
     5821 gcgagtggtt agccaagtta aattttgaag atgtaataaa attagcagca actattactg
     5881 ttgcgagaat gttagaaaga gaagatttta aaaagagata tgaaggacaa atgccaatat
     5941 cagtacatga gttcttctac ccattaatgc aagcatatga ctctatagca ttagaagctg
     6001 atatagaact tggtggaact gaccaaagat tcaatttact aatgggaaga tcattacaaa
     6061 gagaatttgg tatggagtct caaatagtaa taatgatgcc tttaatagaa ggattagatg
     6121 gtaaagaaaa aatgagtaag agtttaggta actatattgg aatagatgaa gaagcgggaa
     6181 taatgtacca aaaatctatg gaaattcctg atgaacttat aattaaatac tacaatttag
     6241 tgacagatgt tcatcctgat gaagttaata aaatagaatc tcagttgaaa gagggttcag
     6301 taaatccaag agatataaag atgaacttag caagagaaat agtaacatta taccatggag
     6361 aagaatctgc taaagaagct gaagaaagat ttaaatctgt attccaaaaa ggtcaaatac
     6421 ctgaagatat acaaacaatt caagttaagg aagatggatt tgacttaatc gaagtactag
     6481 tttctaatga gattgttaag agtaagagtg aagttagaag attagcttct caaggtggag
     6541 taaaggtaaa tggtgaaaaa gtagaggatt taagcactat tgtaaaagag agtgaattag
     6601 ttgttcaaat aggaaagaaa aaatttgtaa aaatcgagct agtaaaatag agtaaagtaa
     6661 aagcaagtct attgagtata aaatgtacat tcaataaact tgctttttta ttatattaaa
     6721 attttaaatt gataaccatt gtctttaaaa tatgttataa tttggtccaa tgcatttact
     6781 gtttcttgtt ttccataagt atcatgcatt aaaagaacaa ccatttcttt gccttcagaa
     6841 gatttctttg catgttcaaa taattcttgt gcatttttct ttttaccttc tgcatcagca
     6901 tttaaagcgt tccaatcaat tgatgccatg tttttctctt ttaagtagtt tcctagaggt
     6961 tccatatttt tccatgacat atacccgcct gggcatctaa ctacatttga ggaaaaattc
     7021 tttcctaaga cttttttcat agcttcatca gttttgttaa gttcatttac gaatgtatcg
     7081 agatttaggc ttctgccagg atatagtttt ttatagtcat gggtataaga gtgatgggct
     7141 attgcatttc cttcgtcaaa tgttcttttt aaaatttcat tagctccttt tctttcaaga
     7201 gaatcaccct taataaagaa agttcctcta acaccatgtc ttttcaagat atctagaact
     7261 tgtggagtat tagtagtaga tggtccatca tcaaatgtaa gaaagactat cttttctcca
     7321 ttgtttgaat agtcgtattt gttaagtttg tcccaaacta tttttgcgtc atatccatat
     7381 tcttttacag cttctttttt tgcaccaata ataggttcat ttttttcttt ttcaattctt
     7441 tctttttcta atctttcagc tttaagtctt tcttgagttt ctttcatttc tttttttcta
     7501 gacatgtccg cccatgccat agaaaaaata ccaataacta ttacaacaag tatagtaagt
     7561 gtaacagttt tcttttttct tttttttacc ataattacag cattccccct tttgtcgaat
     7621 tgtatcaaaa atatattata attatatcga agaaagtaga aaaagtaaac cgacaaagtt
     7681 gtaactaaga taactaaagt gtaacttata tgac
//
LOCUS       DAEPOZ010000176         7700 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.176, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000176 DAEPOZ010000000
VERSION     DAEPOZ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7700
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.176"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..313)
                     /locus_tag="KR440_002829"
     CDS             complement(<1..313)
                     /locus_tag="KR440_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0699842.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KI"
     gene            complement(466..1419)
                     /gene="msrA"
                     /locus_tag="KR440_002830"
     CDS             complement(466..1419)
                     /gene="msrA"
                     /locus_tag="KR440_002830"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBH0699843.1"
                     /translation="MIKLATFAGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRIEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEVLGYGEYLKLFE"
     gene            complement(1543..2448)
                     /locus_tag="KR440_002831"
     CDS             complement(1543..2448)
                     /locus_tag="KR440_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBH0699844.1"
                     /translation="MKSSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            complement(2812..4404)
                     /gene="hcp"
                     /locus_tag="KR440_002832"
                     /gene_synonym="priS"
     CDS             complement(2812..4404)
                     /gene="hcp"
                     /locus_tag="KR440_002832"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBH0699845.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKLG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            complement(4464..5189)
                     /locus_tag="KR440_002833"
     CDS             complement(4464..5189)
                     /locus_tag="KR440_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0699846.1"
                     /translation="MMRKIIKIDEGKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSTSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            5330..6058
                     /locus_tag="KR440_002834"
     CDS             5330..6058
                     /locus_tag="KR440_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBH0699847.1"
                     /translation="MKTNYRLHMQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEM
                     LECLGVVDKTYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLF
                     GEVFACTRLHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKN
                     ILLHNKIELLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRE
                     LGKMRDEGLLSFNKNKFEIFYFQK"
     gene            complement(6222..7493)
                     /locus_tag="KR440_002835"
                     /pseudo
     CDS             complement(6222..7493)
                     /locus_tag="KR440_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416360.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
BASE COUNT         2523 a         1356 c          866 g         2955 t
ORIGIN      
        1 atattttatg tattataatt attttttctc tataagaaat ctcttctact ctaggtagtt
       61 tagattgagg gttaaaatgg ctatcctcta caacgatatt tatattttca tttttataaa
      121 aatactcttt tggaaaattt aatatattag ataattttaa tgtattttct atatttggta
      181 catacttatt ttcctcaaaa gcttttaaat cttttttatt tattttagtt tctttagaca
      241 actgttctat cgtctttgct ctaaaaattc tagctgattt taatctcttt ccattaaagt
      301 cttttaaatt catttgtttt ctctctttca aaattaaatt cttaatctat tggtataaat
      361 aagattacaa ttaataattt atcatattat tgacaaaaaa acaaaaggtg aaaatatttt
      421 tttatatttt caccttttag ataagtttat aataaatatt cctaattact caaaaagttt
      481 taaatattct ccatatccta aaacttccat attttctctt ggtataaatt taagagaagc
      541 agaatttata caatatctta aacctccaag ttcttttgga ccatcatcaa atacatgacc
      601 taaatgagaa tttcctttag cacttcttac ttcaattctg tacatacctt gtgagaaatc
      661 acttctctca atgatagatt tatctataac tggctttgaa aaacttggcc acccacatcc
      721 agaatcaaat ttatcttttg aagaaaataa cacttctcca cttacaatat caacataaat
      781 tccctctttg ttattatcat aatattcatt ttcaaaaggt acttctgtca tatcattttg
      841 agtgacttca aattgtatag gagttaaagt cttttttaac tcttccctat ttatattgtt
      901 tccatcccaa tgacttttta caaaattata tcttccagac cctttataat acatattgta
      961 gtttaatggt ctttttttat aataatcctg atgattttct tctgcttcgt agaatttagt
     1021 tgctggaatt atatcagtca ctattttact atcaaatatt ttactatttt ccagatgttc
     1081 tcttgactct aaagctatat ttttttgttc ttcatcatga taaaatatag cagtcttata
     1141 ttgagttccc ctatcaccaa attgtccacc atcatctgta ggattaatct gtttccagaa
     1201 gatatttaga agttcatcat aacttattat atcatcatca tatgttattt gaattgcttc
     1261 taaatgacca gtagttcctg aacatacctc ttgatatgta gggttttcaa catgaccacc
     1321 tgtatatcca gaaactactt taataactcc atcatactta tcgaaaggtt ttaccataca
     1381 ccaaaaacat ccacctgcaa aagtagcaag ttttatcatt ttcatcactc cttaaataaa
     1441 ttatacttta tttattccct aagaagtaac ttaataatct gcattttatt atttttacct
     1501 actcaatgaa atcatgtgca aaaatacaat tgcaaattga atctaatttc tataaaagct
     1561 ctttttcttt atccatccaa aagcatcttt tatatcacaa ttttctccta aagacattag
     1621 atattctaat gcatcacaat catccatagc tacatctttt tccatcttag gatttaatct
     1681 atagtaatta tctccaagaa gtttttgaga aaataactga ctcatttgag aatttccttc
     1741 taaggtaata gatataattg gtaaatcggg ttctttatta attatccagt caatagctcc
     1801 ccattcagta gtatctgcct ttatactatt atatacatat ccagtcccta ttgaaaaaag
     1861 tcttgtattc ttaagtttaa atccactgtc aatggcatga attatacacg ctaaacttgg
     1921 gtcagttgct attattcctc catcaacatg acgattatat gttggaaaaa atactggagc
     1981 agcactactt gccattgcta catctacaac tttgctagtc tttgtaaaag aatttggcat
     2041 attgttataa aacactggtt tccatgcatt ttcctcattt cctatataaa aacttggtat
     2101 cattactaat ttatttaaat cttttaattc tagttcctct ggaaatatac ttaataaaac
     2161 ttcttttaag tttttgtttt catactttgg tctgagtatt tctgcataac ttttattaaa
     2221 aatatactta gaattttcaa ttgagtaaag ttcttttatc tctttagggc ttacaccata
     2281 cgcaagtcct agtgcaatta gactccctgt agatgtccct ccaaacatat ttatattgtt
     2341 taatagattt ggatactctt ttactatcct ctcaagtatg gatatactca atgccccttt
     2401 taatccacca ccatcaaatg ccaatatatt aaaattagaa cttttcatat acatataccc
     2461 ccacataaaa caccttatat tttaatcaaa ataatttcac tatagtatat atattttatg
     2521 caagttacat attacaatgt tcctctttta atatttaagt tacattcaaa cctttagtac
     2581 ttaaaataaa atatataaga tattatatat ctaagtatta taaatttaat acaaaaattc
     2641 ttaaaagaat atactgaaag caggtttatt tttcaattat tacaaaaaat aaataaaggg
     2701 tcgtctcaaa attgattttt taatcaaatt ttaagtttaa ccccttatct taattgtgac
     2761 atacttaaaa atattttaac tataaatatc tttaagtata gatatattta tttatcctaa
     2821 aatcttcttc atatcttctt caggagtaga tataggtgtc aatgcaaatt tatcaaccaa
     2881 tatatctaat acattcttag ataagaatgc tggcaaagaa ggccctatat agatattttt
     2941 gatacctaaa tgaagtagtg taagaagtat acaaacagct ttctgctcat accatgacag
     3001 taccatgcta agaggcaaat catttactcc acattcaaaa gcatttgcca atgaaactgc
     3061 cactttaatt gcactataag catcattaca ttgtcccata tccattaaac gaggtaagcc
     3121 acctatatcc ccaaggtcta aatcattaaa tctatatttt ccacatgcaa gtgtcaatat
     3181 aacagtatct ttaggagctt gttttacaaa ttcagtgtaa taatttcttc caagttttgc
     3241 accatcacaa cctccaacca ataaaaagtg tcttatagct ccactcttta cagcatctac
     3301 tactttatct tgtacagatg aaactgtacc tcttgcaaaa ccagtagtta atgtatctcc
     3361 tccattaata ccagtcatat gaacatcttc tctatatcct ccaagttcaa gagctttttc
     3421 tattacacta gaaaaatctt tatcttcatc aatatgaacc atacctggat aagcaactac
     3481 ttctgtagta aatactctat ctctataact atctttaaca ggcattaaac agtttgttgt
     3541 aaataatatt ggagcaggaa tattatcaaa ttctttttgt tggttttgcc atgctgtacc
     3601 aaaattacct tttagatgtt tatattcttt tagttttgga tatgcatgtg ctggtaataa
     3661 ctcaccatga gtatatatat ttataccctt atctttcgtt tgttctaata atttgtgtaa
     3721 atcataaagg tcatgtcctg taactacaat aaatggccct ttctcgattt tcatagatac
     3781 ctgagtcggt tctggtgttc cataagtttc agtatttgct ctatcaagca attccataca
     3841 ttttaagttt atttctccag ttttcataac tagacctaaa agttcgtcaa tagacaagtc
     3901 tcctccaact gctattaatc cttcatagaa aaattcattt acgtctttgt ctgtataacc
     3961 aagtaccatt gcatgatatg cataggcagc catgcctctt aatccaaata agattaatga
     4021 ttttaatgaa cgtatatcct cattatcatt ccataaatcc ttcatatcat agttatcatt
     4081 tctattacaa gttgttgaac aatctgaaca tctaggtaca agtttacttt tttctacctc
     4141 agttttttca atttgtcttt ttatagcttc atcatcaaaa cttacattcg ttatagtagt
     4201 gaatagcccc tctatcataa ttttatctgt ttcacttgtt ggtctattct tatttccaat
     4261 agttgctctt gaaagaccaa tcaacactcc tgtaagctcg tcttgcaagt tagctgtacc
     4321 ccccttttta ccacaaacac catttatagt acaaccttta cctcctgctg tttgttcaca
     4381 ttgaaaacaa aacatttttt ccatttttta ttcctccctt tttatttatt aaaatttatt
     4441 atatttttct atgattcact taattaaatt tctccatcta caatttttcc atctacagat
     4501 atagtaacta tttgccatgg tatcattttt ccactatttt gtagagctgt cttaacagca
     4561 tttactatac caccacagca aggaacttcc atacgtgtta ctactatatt tttaatatca
     4621 ttttctttta aaatctctgt cagtttttca gcatagtcaa cttcatcaag tttagggcat
     4681 ccgattaatg taaccttatt tttcataaat ttattatgaa aatttccata tgcatacgct
     4741 gtacagtctg ctgctattaa aagacttgca ttctttaaat atgatgcatt agttggtact
     4801 aattttattt gtactggcca ttgattaagt tgagaacctt ttatctctac tatatcattg
     4861 cttatagatg tactatttga aacctctcta tttattgact tagactgaga acctggacat
     4921 ccacatgcta aagttttctt ttgtgctttc tttgcttcca tatttgcttt tactgcagct
     4981 tcatcatatt ctgctgcttc tctatactca aatgaaatcg catttgttgg acaagttggc
     5041 agacaatctc ccagcccatc acaatagtca tctcttaaaa gttttgcttt tccatttacc
     5101 attcctatag cttcttcatg acaagcttca acacatagtc cacaaccatt acactttccc
     5161 tcatcaattt taattatttt tctcatcatt tatatcacct cataaaattt ttttaattta
     5221 aagtattgat ttcttatata tttattttta atacctatat ttctttattt caaatttctt
     5281 gtagtaggat ttttatatcc ccttgctttt ataaacttag tatagtacaa tgaaaacaaa
     5341 ttatcggttg catatgcaac aaggaagtga ttatttgaaa aaatttttag aaaagcatat
     5401 agacctactt aaaaaaattc ctctatttga aggaattaaa agtgatgagc ttgaagaaat
     5461 gttggaatgt ttaggtgttg tagataagac ttacccaaaa gatagtataa tattctctgt
     5521 tggaagtgaa gttacttcta ttggaattat gcttaaagga agtgctcata taataaagga
     5581 agatatcgaa gggaatagga atattgtagc tgaactcttg cctggagatt tatttggaga
     5641 ggtattcgca tgtactagac tacataaaag taatgttaca gtaaccacaa catcttcttg
     5701 cgaagtttta tttataaagt ttaaaagtgt aactggtatt tgttcatctg catgtgtatt
     5761 tcataatagg cttgttgaaa atatgctaca attaatagct gaaaaaaaca ttttacttca
     5821 caataaaata gaattacttt ctcgtaaaac aactcgcgag aaattgctta tgtatttttc
     5881 aaaacagata gaacaaacag gtagccacca gtttacaatt cccttttcaa gaaatgaaat
     5941 ggctgatttt ttatgtgttg acagaagttc tatgtcaaga gagcttggca agatgcgaga
     6001 tgaaggcttg ttatctttta ataaaaataa atttgaaatc ttttactttc aaaaataaac
     6061 tagatattta aacatattta tttcaatata tactatatta gttaattcaa attataaata
     6121 tcatatttga atcttaaatt tatattttta cttaaaaagt taatagatat ctaatttatt
     6181 aaaattaaga tacctattaa ctttattaat taagtgtaca attagcttat tttctttgta
     6241 gtaattgctg ttttatctat aagccaatct tttccttcta ctaatcttga aactaacatt
     6301 gatgatactg tatttccagt tgtatttaat atagtattag gaatatcagt tatagtagtt
     6361 ataactgtta acaatggaat tgcctccact ggaaaaccga agatgctaac cacgagcatc
     6421 tcaccaataa gtcagccacc tgctacagga gccataaata ctccaacaac aaatacagtt
     6481 ataaataatt gacctatcgt tgctggattt gccattccac tgcctaaaat acctgataaa
     6541 aatgccactt ttattattac tgctaaaact gacccatctt tatgtatatt tattccaaga
     6601 ggtataactg tttctgatat atcatcagga actcctatat ttttactagc ttctaaattt
     6661 gatggtatac atgctgaact tgaagaagta gctagtgccg ttgctattgg ttttatagca
     6721 tttctccaga attgtctaaa cccttgtttt cctgcagata aaaaagcata cagactgtaa
     6781 aataataaga aatacatggc tgctgttagt gaataaatta caaacaccct tacatatcct
     6841 tgaattattt tgcttccaag ttccccaatt attgtagctg tataacagcc tagccctata
     6901 ggagcataaa gcatgactat acttactact tttaatattg cttccgttcc ttctgataaa
     6961 aactttctta ttgtatttcc cctatctccc atcatagcta ttgctgttcc aactaaaact
     7021 gcaaatataa ctaattgtag taaattgcta ctggaaaata aatctccaaa atttttaact
     7081 gtaatacttg atactacttg gtctaataac gttccttttg attctaaagt ttctgcatct
     7141 tgagccacca ttaaactttt tacacttgat acatctaatc ctttagacgg attaaataat
     7201 aatgcagatg taatacctaa aaatgcaatt acaagtgttg ttcccataaa tactatagct
     7261 gtactcttca ttatttttcc aaaacgttcc atatttttta tactagcaac acctgaagaa
     7321 acactaagaa aaactaatgg tactactatc attaatagag catttatgaa taaatctcca
     7381 aatggtttta gtattgaagc ttttggacca agtactaatc ctactatcac ccctattata
     7441 gtacatgcta aaagtataat tgatgactta taatttttta aaaagtccct catttatatt
     7501 ttcctttcta atgttttata ttataaatta ccctaaatcc taaaaaaatt aaagtaaatt
     7561 atctaatgcg cattaataat ttttgtaatt ttccccaaca gctactttag caaatactgg
     7621 aatatatgtc aatttctata gtttttttgt atctttaata ttttattaat agttcatatc
     7681 tatatataaa tattaataaa
//
LOCUS       DAEPOZ010000177         7671 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.177, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000177 DAEPOZ010000000
VERSION     DAEPOZ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7671
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.177"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(208..2082)
                     /locus_tag="KR440_002836"
     CDS             complement(208..2082)
                     /locus_tag="KR440_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0699848.1"
                     /translation="MNKYNKIANELIEIIGENNIISITHCATRLRVMVKDREIINDKK
                     VEKVNEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEVEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIGLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVFSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDIA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(2087..3751)
                     /locus_tag="KR440_002837"
     CDS             complement(2087..3751)
                     /locus_tag="KR440_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBH0699849.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDATPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSKYSDCLIYGSIEPIDIESDNVIAYRRQLDLVVIDCY
                     YNFSDKLVEIKTEEKYDIVFQNLDDINLLNKTVSLKPFQAVLLKVR"
     gene            complement(3765..4586)
                     /locus_tag="KR440_002838"
     CDS             complement(3765..4586)
                     /locus_tag="KR440_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBH0699850.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSIGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDVHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(4685..5449)
                     /locus_tag="KR440_002839"
     CDS             complement(4685..5449)
                     /locus_tag="KR440_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH0699851.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVVKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDTSTIIDFAKEMRMKGTYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(5446..6195)
                     /locus_tag="KR440_002840"
     CDS             complement(5446..6195)
                     /locus_tag="KR440_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBH0699852.1"
                     /translation="MRLESLVNQSYNSLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKSGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(6699..7304)
                     /locus_tag="KR440_002841"
     CDS             complement(6699..7304)
                     /locus_tag="KR440_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0699853.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMKIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
     gene            7483..7581
                     /locus_tag="KR440_002842"
     CDS             7483..7581
                     /locus_tag="KR440_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBH0699854.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFD"
BASE COUNT         2628 a         1349 c          793 g         2901 t
ORIGIN      
        1 ctccctcaca tcctcaattg ttgccactat ttaatttttt gtatgaagtt aaatatattt
       61 tcatcatttc aacaatttgg gtgcagtaca atctctattt tgagacaacc tctttaattg
      121 ttttagagtt tatgaattat ttctcatact ttaaaaagat tgaattactc ttgataatct
      181 tcttttgtag tcattacata tcacctacta ctcaacacat attctttgac ctatttttac
      241 atccttgttt atatccaaaa tatttacctt cttaccatct ggaaacaata ccattgtcaa
      301 agtagacttt cctaattcat gtattctctt taaatcaaca ttaacaagaa catctccctg
      361 tttaaccata tctccttgtt taacagcaat atcaaagcca tctccattta atgcaactgt
      421 atcaattcca acatgaatta atacttctcc attagttcct ttaatcccaa atgcatgtcc
      481 acttgaaaat actgtttcta atttacctga aataggagaa acaatttttc catctataat
      541 atcaatagca aaaccatcac ctaacatttt tttagaaaat gtttcatctg gtacattttc
      601 taatgcaaca attttcccct tagctggaga cacaaagttt tcattatttt cattaaacac
      661 taattcttcg tcttcatctt catttatagc ttctttaggc actccaaata aacatgttac
      721 aataaagcag aatgcaacca caaatattga tactaataag tatgtgatta aattatatcc
      781 atcatagata tacattaaat aagaaggtaa aactgctaat ccatatgaat tagattgaat
      841 atgtaaaatt gataatattg ctgctcctaa acctgaagat gcacacataa taatcaaagg
      901 ctttaaatta tatcgaagct gaatcccaaa taaaacaggc tcacttatac caaaaagttg
      961 agatagcatt gctccaactg cagtttgttt aacttgatta tttttagatt ttttcataaa
     1021 tgctagacat gttccaatat tcgcaaatcc atacattgca caaagagtaa taagtgcatt
     1081 aaatcctgta tttgctaata gtgatgtttc aatcattaca taagtatgat gtaagccagt
     1141 aataacagct aatggatatg taaatccaat aataaatcct ccaattccta aaggtaaacc
     1201 aatcaataaa ctgatgatat caaccatttt taattctatt gtatgcatta taggccctac
     1261 acctaacaaa ataactaaca ttgttattag cataacaaag aatggtgtga aaataaggtc
     1321 taatacatta ggcatatgtt tacggaacca tttttcaaga ttagctccaa taaatgctgt
     1381 aacaatagct gttaaaacac ttccttgaca gcctacaata ggaataaatc caaatgccat
     1441 aattgcttca acttcatttg atgggtcagc aacagaatat gcattaggaa gtattggaga
     1501 aactaacata agtccaataa caagtccaat aacaggtgtt cctccaaata ccttaaatgc
     1561 tgaccataca ataattgctg gtaaaaaagc aaatgctgtt tctgtaagaa ccgaaacaag
     1621 tgtctgaata tacaatggaa tatttgcaga agacattcca aataatccaa gtacattatc
     1681 attaaacaaa caacccttta atcccaagaa taatcctgtt gccgcaataa ctggaataat
     1741 aggaacaaat atatctgcta gagttctcat aactttctta aatccagttt cattattttt
     1801 aactaattca tcttgttctt tttttgataa agttttcaat cccatttttt caacttctgc
     1861 ataaacttta ttgactattc ccgttcctag aataatttga tactgtccag aagtaaagaa
     1921 aacaccttta acctcattaa ccttttccac tttcttatca ttaataattt ctctatcttt
     1981 aaccatcacc cttaatcttg tggcacaatg tgtaatagaa ataatattat tttcaccaat
     2041 aatttcaata agctcatttg ctattttatt atatttattc atttttctac ctcactttca
     2101 ataatactgc ttgaaatggt ttcaaactca ctgttttatt tagtagattt atatcatcta
     2161 aattttggaa tacaatatca tatttttctt ctgtttttat ttctacaagt ttatcagaaa
     2221 aattataata acaatcaata actaccaaat ctaattgcct tctatacgca ataacattgt
     2281 cactttctat atcaataggt tctattgaac cataaatcaa acaatcagaa tacttactat
     2341 cttgtctcaa ctttatcatc tttttataaa attctaaaat actttctgaa ttgttaatct
     2401 gactctgtgc attaatatct ttataatttt caataacctt taaccatggt gttgcatctg
     2461 aaaaacctgc attttcttta tctgcccatg gaaatggcgt tcttgcatta tctcggcttc
     2521 ttaaattcac aaattctaac gcttcttctt ctgagtaacc ttcatctatt gaacgatagt
     2581 attggtcaat gctagataaa tcattaaaat catttatggt atctctttta aaatttgtca
     2641 ttcctaattc ctgtccctga taaataaatg gtgttcctct taaaaagaaa tacattgctc
     2701 ccaacatttt tactgcatca tcattccttt cgtattgcaa aagatacttt gttgtagctc
     2761 ttggttggtc atgattttca ataaaattag ctgcccatcc atgttcctga acatacattt
     2821 ggctatctaa aatgcatttt cttaaatctg gaattgtcca tgaaattctt ttaaaccact
     2881 cactaccaga agcaacatct aagtcagcat gtttaaagtc aaagaccatt gaaaagaaac
     2941 catcttctcc aataaaatca cctagttggt catagctaat acctgctgct tcagcaacag
     3001 tcatacaatc atatttttta aacgtatttt cttttaattc ataaagcatt ttttctattc
     3061 caggctgatt tctaactgtt tttgtacatt taacaagacc atcaacacca tcactttcta
     3121 aactttgaaa acttaaattc tttttgataa atgtaatagc atcaactcta aatcctgcca
     3181 ctcccatttg agaccaggta ttaatcattt ggtaaagttc acttcttaat attggatttt
     3241 cccaattaag gtcaggttgt tttttaccaa aagaatgaaa ataatattcg tctctattct
     3301 ctaccttttc ccaaacactt ccaccaaaaa cagacctcca gttatttggt aaatcgtttc
     3361 cttttttaaa aatataataa tcatgataag gactttctgg attttttaat gcttcttgaa
     3421 accaaggatg ttcatcactt gtatgattaa gaactaaatc taatataatt ttgattcctc
     3481 tatctttact ttctttaatt aactctttta aatcttccat acttccaaat tcacttgcaa
     3541 tatctaaata atcactaata tcatacccat tatcgtccat aggtgattta aaaattggac
     3601 ataaccacaa tgatgtaata cctaaatctt ttaaataatc taaacgagaa ataattccct
     3661 gtaaatctcc aattccatca ttattactat cttgaaagct tcttggataa atttgataaa
     3721 cgatttcttt ttgccaccat ttcttttcca taaattactc ctttttatat gatatttctt
     3781 ctttttaatt ctttataaat tccattatcg aatatgtcct cacatataga tgtcgctata
     3841 tctttaagtt gttgatgact atttttcatt gcaattgtaa cgtctgatgc ttgaaacatg
     3901 acaatatcat tttgaccatc accaaaacat atggtttctt tttgagttac ccctaatctt
     3961 tcttgtaatc tcttaattgc tttgccctta tgaaaatctt tttgaataat ttcataatac
     4021 tgactagaaa tatctctctg tgctaattcc attttatctt gtagtatatc tttcacttcg
     4081 tcaaatacct tttcatttga ccataaacaa attttatgta catcttgtga cttatactct
     4141 tcaatattat tttcatatgt tattttttct tggatatgtt gtttatttat acaagaatta
     4201 gttcctttca actgattcat tgtttcaaat atttctttgg ctttttgatt catgaaaact
     4261 ttttcctgac tctcaatcga aaaagcaact tctctctttt ttaacaaaca aacaacttcc
     4321 tttatcaatc tttgattaaa agattgatta taaagtaatt caccatgata ttgaatgtaa
     4381 ttacctcctc ctgcaatata tccatcaacc cctaatgata aaacatcatc ttgtattgta
     4441 cctatacttc ttccagtaca aataacaaca ctgcaatgat tcttttgaca taaacgtata
     4501 gcgtgctttg cagattctgg gattccatat acctcatccc taagcgtacc atctatatca
     4561 agtattaata atttgtacaa tttcatcttc ctttcttact atcaaaatca taatagcatt
     4621 caaaaaaaaa gatatcaata ttttgatatc ttttaaaaaa tatgttcatt tttagcacaa
     4681 attttcactt tcggggaata tgttcacttt ctaaatacat cttgtattta atataaagca
     4741 gttcaattaa aataaaataa ggtgttgtta catcgtagtc cagttgttta tctacagaaa
     4801 tagatataga ttgaatatac aggttttcat cacataatct tgctaaagga ttacttatca
     4861 tttttgtaat agaaaccata taagttcctt tcattctcat ttctttagca aaatcaataa
     4921 ttgtagatgt atctccattc aatgagataa agaaaatcat atcatctgtt tttataaaat
     4981 gattaagcgc ctgaatcata tcataaccat aaacatcata tataattttc cctgttggta
     5041 agaaaatcct tttcatctct ttaacaactc tttcctgaga ataaccttct ccaaagacga
     5101 ttatacgatt tgcctttttc attctttcaa acataatcga acaatccttc ttctctatat
     5161 catctatcac cttattataa caatccatta attggtgttt actttgattt ttatattcta
     5221 cttctgtttc atttaagcgt ataataactc ttaattcact atatccaggt aattgtagtt
     5281 tttgagcaaa acgagaaagt gcacttgtag atatatggta tttgtctgca aattcatcta
     5341 tagaaagttt aatacaatct tctttatgat taagaataca ctcacagata tacttatcat
     5401 ttgctgtaaa tgaattataa tatttattga aaagtccttc aattttcatt cttttctcct
     5461 tctatactct atgtaattca tatgaagcaa atctaataga gcataaaatg ctcctacgac
     5521 ttcataagat atagcttgta atgtatcaag tttttgagtt tcaacataca gattatactc
     5581 acaaagtgtt gcaattgtat tattatcaag cctagttaaa gaaatcgtat gtatatgatt
     5641 gttgataaat tttgcaattt caattcctga ttttgtttct ccagacaaag aaatgataat
     5701 aaataaatca cttttttgaa atgattctct cataaattca atttcaccta tatcaaataa
     5761 gtcaataaca catttaccta ctgaaagaaa tattctcttt atttcttgag ctaaactttt
     5821 ctgttcatta cctgtcccat aaatataaat tgtttctgca tcatatatta ttttacaaat
     5881 atcttgaaat gatatttttt gtaattcatt cattaatagt cgataattct cgcaaacatc
     5941 atgaaaatta ttatcattat taacaaattc tagttgatta tttttaagta aatactttaa
     6001 atcagagaat gaatctaatc caatttttct acaaagcctt aataaagtag ctctagaaac
     6061 attacattca tttgcaattt tttcacacga aaaattacgg tattctcttt gacaaatcaa
     6121 tatttgttta ataataattt tatcattttc atttaaacta ttataacttt ggttaactaa
     6181 cgattctaat ctcatttttt ccactcccta aatattattt taatatacat tagttgcttt
     6241 ttctagtgaa gagtcttaaa taataaggat aaaataatca tttgatttta tttttattaa
     6301 aataaaaata acctgctaaa agcaggcacc aaaaagtact tatcctttcg aacatgtact
     6361 ttagatattg ctttcttcgg aagcttcatc tcttcatact ctaaaataga gttttttcta
     6421 actcaaaagt attttccatg gtagaaatta ctttaattca tctgccattt attttttatc
     6481 ttatttaata attactatta cttgaatatt tacttcttta agtaaatttc atagttaaca
     6541 aagctaaaac tattattata gatgttaaaa tacaaaatta taagtattgt tataaatatg
     6601 cttacagcta caaatataca tgatatatta aaaagaagct gtaaattatt atcatacata
     6661 caagcaacta tgcctctatt aatccatgtt aatttttatt atttaataca aggagttcca
     6721 tgagacatac atatccattc atattttaaa ttatccaatt ctcttataga tttcatcatc
     6781 tttttataat cccaattcca tggattagga taaggaacta tatttccatt cttatttttg
     6841 actaaatcac ctgcaaataa tacatcttca aaaatcatgc agacatgccc tggagtatga
     6901 ccaggagtat ggataatttt tatattccca atcttcatat tctctccata aggttcaaca
     6961 tccttaatga gccttctgga aattgccttt ccaataaact tcttaaaccc aggtctatcc
     7021 ttctctccta taataaaagg tatatcctct acatgagccc atacctttgc tcctgttttc
     7081 tctttcaata atttaatatt agatatatga tcaacatcat gatgagttaa taaaatatgc
     7141 ttaatatctg taagtttaat gccaagtgtt tttaattctt taattatatt tttccccata
     7201 aaaggaagac ctgtgtctat aagaataatt tctttatcga acacaataaa acaatttgaa
     7261 ccacgagtac tatctaacat taatacattt tcttttatct tcataaataa attcccctta
     7321 tttggtattt tacctatata attaattaat aaagttataa atagttataa tacttgatat
     7381 taattttatt ttttattaaa gtatactata ggtatacaaa tattatattt acataagtat
     7441 accatcagta tactttttta tcaatagaaa ggaataattt atatgtcaga attatcaaaa
     7501 agagataaag aaaaaataca aagagaaaat gagattattg ataaggcaga aaaattattt
     7561 tgtcttaatg gatttgatta aaaaatagct acgcacactc gtgactttag tcatgagtta
     7621 gtagctaaaa tagtcagcat atagggaaac ctatatgtag agataggata a
//
LOCUS       DAEPOZ010000178         7627 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.178, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000178 DAEPOZ010000000
VERSION     DAEPOZ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7627
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.178"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..843)
                     /locus_tag="KR440_002843"
     CDS             complement(<1..843)
                     /locus_tag="KR440_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HBH0699855.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIGLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLN"
     gene            1103..2092
                     /locus_tag="KR440_002844"
     CDS             1103..2092
                     /locus_tag="KR440_002844"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBH0699856.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEVHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            2164..2904
                     /locus_tag="KR440_002845"
     CDS             2164..2904
                     /locus_tag="KR440_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HBH0699857.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPPLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            3104..4255
                     /locus_tag="KR440_002846"
     CDS             3104..4255
                     /locus_tag="KR440_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBH0699858.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLEPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            4280..5035
                     /locus_tag="KR440_002847"
     CDS             4280..5035
                     /locus_tag="KR440_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HBH0699859.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKNTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            5227..7614
                     /locus_tag="KR440_002848"
     CDS             5227..7614
                     /locus_tag="KR440_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HBH0699860.1"
                     /translation="MKKFTSKKVARFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SDTYKQYLNDAKNGRTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSVKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMDGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVSYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFNIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
BASE COUNT         3060 a          835 c         1358 g         2374 t
ORIGIN      
        1 atttaaattt ctaaaaataa cagctgaaaa catacaagtt acaacagagg gtaatagtat
       61 attaaacgtt atatagaagc atctacttat aatttctcct gtatttatac aaataaatat
      121 gagaagaacc tgttccaaaa atacagatgg tgttattgtt tcggagaagt tattattttt
      181 tatcctattc ttatttctaa aaaatttact tccaaatctc tttagatttc tattatatct
      241 aggaatcgac aagcctatat ttgatgatgg ttttaattta tatttattta ttagaaattt
      301 agctacagga catcccaata taccacccat aataagtcct attgttgcag cagataatcc
      361 aaccccaact gctccttcta taccgagatt ttctattgta agtccaaatg cagcagaata
      421 accatgacca ccttccatag atacatttcc acacataagc cctaaaagag gttcaagttt
      481 tataatcttt gatattgtaa tagctaatat attttgacaa aatgccaata ctccacacaa
      541 aatccaatat ctaaataata cactaccacc tttctttata agatttatgc ttatttcaag
      601 tcctatagtt gtaaaaaaga aggttataaa atttggcatt aaatttccac ttatagatat
      661 atctgctata ttaaaatatt taaaaaataa atttagaaaa caaaataata gacctcctat
      721 tactggtgca ggtatacaga aattatttaa aaagttaact ttattcttca ataaattacc
      781 aattaaaaag aagaatatag atagtgtcaa ggtttgcact atatcaaaac ttaaggtaat
      841 caaaataagt cctcctttgt aattcaaagt tttaattata taacttaaat tcttatgtat
      901 tgaaatttta ttatcaaatg tatattaata aatgtatatt aaaaagaatc agtatatata
      961 attagatagt cataaattaa attaaaatat tttaatgaga tgtgactttt ttagaatgaa
     1021 aaaccgtagt atattgatat aatagaaata agacttaaaa atctgttgat agtattattt
     1081 ataagtacag taggaggata aaatgtttga aaatttacct aagatagatt tacattgcca
     1141 cttagatgga agtgttagag tagaaaccat gcttgatata gctattaaag agaaaataga
     1201 tttaccttca aataatatgg atgaaataaa aaaattagca aaggtttctt ttaattgtac
     1261 ttcactagat gaatatttag aaaaatttga tttaccctta aaagtaatgc aatctaagga
     1321 aaatttaaaa agaattactt ttgaactttt ggaagatgca agtagagaaa atgtaaaata
     1381 tattgaaatt agatttgcac cattacttca cacccaaaaa ggtatgagtg ttaaaaatat
     1441 aattgagggt ataattgaag gaattagaga agctgaaagt atttatgata taaaaggcaa
     1501 tttaatatta ggttgtatga gaacaatgac ctcaaaagag gcattattag taattgaaga
     1561 agggaaatca tttgtaaata aaggtgttgt agcagtagac ttgtgtggtc cagagaaaga
     1621 gggattttgt aaggaatata aagatgtttt taaattagca agagaatatg gatataaagt
     1681 tacaatacat gcaggagaag ctgcaagtgg agaaaatgta ttggatgcta taaatatttt
     1741 aaaggcggat aggattggtc atggggtaaa aattaaagac cataaaaagg cgtataattt
     1801 agttaaagat aaaaaaatat tgcttgaatt atgtccaact agtaatgtac aaactaagac
     1861 agtagattct tatgaggtac atccatttta tactttttat aaagataatt tacatgtaag
     1921 tataaatact gataatagaa ctgtatcaga tataaattta agttcagaac taaatgttat
     1981 atttgatact tttaaactag gattagaaga ctataaaatt atttatagaa atgcagtaga
     2041 agctagtttt gcagataaag aaactaaaga gtatttgaat agtttaattt aatagataaa
     2101 aatatttaaa ctttaaatta agctattaaa ttgacaaaaa tacattgtat taggagaaaa
     2161 aatgtgaaag attatgaaga tgttgtttat attaaactag tcaaaaaaga acttgaagaa
     2221 tggaagaaaa atatattaaa gaaaccttct atatttgaca aagcatcaaa gggtatacag
     2281 aataaattta atggagtatt gccagataaa tatcatgaaa taattacttc ttctataaaa
     2341 gtaatgacaa aaaccgtact gtttggatat aaatatgcta caaagccacc acttagaaat
     2401 atgtcattag aagaaagaga aaaattagtt ttagaaaaag ctaaaactta caagaccaca
     2461 gcaatgattg aaggggcagg aacaggggca ggaggaatcc tcttaggttt atctgatttt
     2521 cctttgcttt taggtattaa agtgaaattt ttatatgatg tagcttcaat atatggatat
     2581 gatgttaaag attatagaga aaggatttat atattaaaca tattacagtt ggctttttct
     2641 agtcaacagc atataaacaa aacttttaaa ttgatgaata actgggatga atatgtaaat
     2701 agtctaccag aagacatcaa tgattttgat tggagaacat ttcaacaaga gtatagagat
     2761 tatttagatt tagctaagtt tttacaacta atgcctggaa taggagcatt tgtaggattt
     2821 tatgtgaatg gaaaattaat tgataaatta tgtgaaactg ctgtgtctgc ttatagattg
     2881 aggattcgag agcttaagtt ataagagaaa ttataacata aaaaattagt tatacaatat
     2941 atctatatgt atctaatttt gcattgataa aaacaattat ggataaaagg taaagcatga
     3001 tagaatctaa tgtagaggtt tgtattttaa tggaaaaatt tagtttttat tgcttaagat
     3061 acaaattgaa ttacgaagag aagtaattgg gaggaatatt ttcatgacaa atttgccaaa
     3121 acaatttgat agcttcaatg aagctagaca aaagggattt ataaatgcaa aggaacttaa
     3181 ggaaagtggg aaaaagatgg ttggagtttt ctgtacattt actccagttg aaattccaat
     3241 ggcagctgga gcaactgtag taggtgtatg cggtgttagt gaagagccaa tacctgatgc
     3301 agaaagggtg cttccaagaa atctttgtcc tttaattaaa tcaagctatg gacatgcaat
     3361 aacagacacc tgtccttatt tctatttttc tgatttgttg ataggagaaa ccacttgtga
     3421 tggtaagaaa aaaatgtatg aagaacttgc aaaagttaag ccaacatatg taatgcattt
     3481 gccaaatact tcaaaaggag aatttgccta taaactttgg aaagatgaaa tgataagatt
     3541 aaaagaagag gttgagaaat ctttaggtgt aactataact gaagaagata taagaactgc
     3601 tataaaagat aaaaatgaag aaagagaact tttaaaagag ttttatgcac ttggtaaatt
     3661 agaaccatct gcacttacag gccttgagtt acataatgtt ttatatcaag caggttttaa
     3721 atttgaccgt gcagaactta aacattcttt aagaaaagtt atagatgata tgaaagaaag
     3781 atatgaaaaa ggtgaatgtc ctgttcaaaa agataagcca agaatattga taacaggttc
     3841 tcctattggt ggaataagcg aaaaaatagt taaaacttta gaagatgcag gggcatcagt
     3901 cgttgcatat gaactttgtg gagctattag atcaaacgat ttattggttg atgaagaaat
     3961 agaagatgta tatgatgctt taacacaaaa atatataaat ataggttgtt cttgtatgat
     4021 gaataatgac aatcgtattg aattattaga tagaataata gatgaatata atgtagatgc
     4081 tgtaatagat gtagttttac aagcatgtca tacatttaat atagaaagtt atagaataag
     4141 agaatttgtt acaaaagaaa aaaataagcc atttatgtct cttgaaactg attattcaaa
     4201 atcagatact gaacaattaa gaactagatt tgaagcgttt gtagagatgt tgtaggaata
     4261 aataaaagta ggaggaacta tgtttagtat aggagttgac tcagggtcag tggcaacaaa
     4321 gggtgtgcta tttgatggtg aaaaaattat taagaaaata attataccaa ctggatggag
     4381 ccctaaaaat acatctaaac aggtctatga gttgctaagt agtgaaattg acaaaaaaga
     4441 tataaaaaag gtggtaggta caggttatgg aaggggagta atggattttg ctgataagaa
     4501 agtaacagaa ataacttgtc atactagagg tatttacttt ttaaacaaga acataaggac
     4561 tattttagat gtaggtggtc aagatagtaa agttataaat ttagatagag atggaaatgt
     4621 atttaacttt ataatgaatg ataagtgtgc tgcaggaact ggtcgatttt tagagattac
     4681 ttctaacctt ttaggaagcg atattgaaag tatagataca ttagctaaag gatatgaacc
     4741 agttaatata tctagtatgt gtacagtttt tgctgagtct gaaattgtaa gtcttctagc
     4801 tcaaaacatt tctactggag aggtagcagc agggatactt aaatctattg caaataaatc
     4861 cacctcaatg ttagcaagag gtgaggtaat agatgaagtt gcatttactg gagggcttgc
     4921 aaaaagtaaa gaactagtaa aaatgataga agaaatactg ggtaaaaaaa tttttattgc
     4981 agaagatact caaataatag gagctttagg agctgctgta ataggattta gataaaaaca
     5041 tataaaaaga aaatgaaaag ctgaatttat cataaataag ttcagttttt tattttattt
     5101 tgtttattta taaaggaaaa ctttggtata attgatttaa atgattgtat atttgtaaat
     5161 acttgatatt tagtcatata taacattaga attttaaaaa ttagctcaaa ataaggggga
     5221 gaaagcgtga aaaaatttac ttcaaaaaaa gtagcaaggt ttgttgcact gtttttaata
     5281 tctattttag taatgtcaac aattcctgtg tcagctgaca atagcaatac attgaatcag
     5341 ataaaaaaag cagaatacag tgatacttat aaacaatatc taaatgatgc taaaaatgga
     5401 cgtacagaaa aatataatgg aattatacca aatccatatc agttagaagg tactcaaatt
     5461 caaagtaaag gaagaacagc tccaacatct tatgacccta gaaaattagg tcttatgaca
     5521 tcagtaaaaa atcaagagga tttaggaata tgctgggatt ttgctgcaat ggcaaccttg
     5581 gaaagtttct tgaagttaaa taattatgga gactacgatt tatcagaaga acacttaaga
     5641 tggtgggctt cagatggtga atatggatgg tctgtaaatg atatggatgg tgctttaaat
     5701 tatgaagcta tgggatattt aacatcttgg tcaggtccta aattagaaaa agatatccca
     5761 tacaatggta gagtatctaa agcacaagga gcaaaaaaac caactaatat gaatacagca
     5821 cctactgttt ttaatgtgac agatgctgtg tgtgtaagta atgacataaa ttctacaaaa
     5881 aatgctatat tacagtatgg agcagtaaca tctggttatt atgaagatat taaatatcaa
     5941 agtgatgacc aaaattcata ttattgtcca acaaaaagtt ttaatacaaa tcatgctatt
     6001 tcaattgtgg gttgggatga taactattct aaagataatt ttaatgcaaa tattagacca
     6061 tcaaaaaatg gtgcttggtt aataaaaaat agctggggag attataattc tgagggtgga
     6121 tatttctgga tatcttatga agacaagaca cttatgtctg atatagataa tttttcaatc
     6181 aaaggtggta aaaaaccaaa tgatgatgag aagatgtatc aacatgacta tgcaagtatt
     6241 gtacctttaa tttcaaaaaa gataacagct gcaaatgtat ttgatttcaa tagaggcgat
     6301 gaaactctaa aatctgtaat gttcttaact gaaagtatag gagctaagta tgaagtatct
     6361 tatgctccag tagtaaatgg tatacctcaa gaaaacaata tgaaaaaatt aaaagaaggt
     6421 actgcccaat attcaggata cataacagtt ccaattgatt cttttaatat accagaagga
     6481 aagggtgcaa ttgtagtcag tatagagggg aaaaatggag agtcaactat aggttctgag
     6541 tcaaatgtac caggatatga tacatttaaa gcaaaagcta atttaggtga aagttatatc
     6601 atagataata ctggaaaatt ctttgatata aatagagata gtaacttcta tccgtgtaat
     6661 tttactataa aagctgttac agaaaagtct tctggtgaga gtatcccgaa tgagtcttta
     6721 attggtagtg atagatatga aactgctatc aaagttagtc aaaatggttt caattcttca
     6781 gaaaatgtag ttttagtaaa tggcagttca atagtagatg cattggcagc aactcctttt
     6841 acatctgcta taaattcacc aatattattg acacaaaaag aagctttaaa ttcaaaaact
     6901 aaagctgaga tacagagatt aggtgctaag aaagtatacc taataggcgg agaaaattct
     6961 ataagtaaag aaattgaaca acaattaaaa agcttaaata tatcgataga gagaatatca
     7021 ggaagtgata gatacaagac aagtttatta ttagctcaaa agttaaatgg tataaaaaat
     7081 gtatctcaaa tagcagttgt aaatggagtg aaaggattag ctgatgcaat tagtgtaggt
     7141 gctgctgcag cagaaaataa cataccaatc atacttgcaa atgaaaagag cgaattgcaa
     7201 ggagctgatg aatttctaaa ttcattaaat atagagaaat cttatattat tggaggaaca
     7261 gcatccctat caaacaatct agaaagtaag ttaaaaaatc caactagatt atctggtagt
     7321 agtagagacg aaactaattc aaagataata gataattttt ataaaaaaga tactttaaaa
     7381 aatgcatttg tagttaaaaa tggaatcaag aatcaaaatg atttaataga tggattggca
     7441 gttggtcctt taggagcaaa aactgggtca ccagtaattt tagttggtga taaattagct
     7501 gatagccaaa aagaagttct taaaaataag actttagaaa aagtaactca agttggtgga
     7561 ggagcaaata aaaatgcatt taaagagtta gtaaaattaa aaagtgctaa atagagtact
     7621 taagtat
//
LOCUS       DAEPOZ010000179         7610 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.179, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000179 DAEPOZ010000000
VERSION     DAEPOZ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7610
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.179"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..1006
                     /locus_tag="KR440_002849"
     CDS             <1..1006
                     /locus_tag="KR440_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HBH0699861.1"
                     /translation="ADSSGYCIFHKENKSDEEIQLMMDTIHKEEISEFNGFVFENEFN
                     AEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTEFRDKVLFNGCIFLENCDF
                     NRTIFSKNYINDRIFEKVKFKGPDLVVNKVENFPRMDGIIFSMCTKFVLKNVEYGKSE
                     YEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMKRSNYPTFSDYLVEKFFDQ
                     IARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNSTLVALDINNIGDYSLSEI
                     FKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFFGVTIGAIWASVIIKRMIR
                     "
     gene            1110..2294
                     /locus_tag="KR440_002850"
     CDS             1110..2294
                     /locus_tag="KR440_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBH0699862.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADGKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHICKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVVNKNEKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGMKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGVIKYYTSSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            2501..3214
                     /gene="rpsB"
                     /locus_tag="KR440_002851"
     CDS             2501..3214
                     /gene="rpsB"
                     /locus_tag="KR440_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBH0699863.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            3293..4204
                     /locus_tag="KR440_002852"
     CDS             3293..4204
                     /locus_tag="KR440_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBH0699864.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            4289..4993
                     /locus_tag="KR440_002853"
     CDS             4289..4993
                     /locus_tag="KR440_002853"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBH0699865.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            5019..5576
                     /gene="frr"
                     /locus_tag="KR440_002854"
     CDS             5019..5576
                     /gene="frr"
                     /locus_tag="KR440_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBH0699866.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            5579..5782
                     /locus_tag="KR440_002855"
     CDS             5579..5782
                     /locus_tag="KR440_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699867.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            5811..6545
                     /locus_tag="KR440_002856"
     CDS             5811..6545
                     /locus_tag="KR440_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBH0699868.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            6558..7337
                     /locus_tag="KR440_002857"
     CDS             6558..7337
                     /locus_tag="KR440_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBH0699869.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         3123 a          784 c         1444 g         2259 t
ORIGIN      
        1 agcagattct tctggatatt gtatctttca taaagaaaat aagtctgatg aagagataca
       61 gttgatgatg gatactattc ataaagaaga gataagtgaa tttaatggat ttgtttttga
      121 aaatgaattt aatgcggaag aaatattaac ttacaattat aaaatactgg atttttctga
      181 atccatattt aaacaaaaag caaattttaa aaaatatatt tttaaaaaga atattatttt
      241 taactataca gaatttagag ataaagtttt atttaatggt tgtatattct tagaaaactg
      301 tgattttaat agaaccatat ttagtaaaaa ctatataaat gaccgtatat ttgaaaaagt
      361 gaaatttaaa ggacctgatt tggtagttaa taaagtagaa aattttccac gaatggatgg
      421 aataattttt agcatgtgta caaaatttgt gttaaagaat gttgaatatg gaaaaagtga
      481 gtatgagcat ggcaagataa attatagaat agctagaaat caagcaacaa aaattggaga
      541 atatgagatg ataggatttt actattataa ggaaagaatt tatagtagta aaataatgaa
      601 acgttctaat tatcctactt tcagtgatta tttagttgaa aaattttttg accaaatagc
      661 acgttacaca acaggttatg gagaaaagcc atggaatata ttattagtga ttatagcaat
      721 aataagtgtt tttgctttat tatacttatt tgtaggcata gaaagttcaa atagcacact
      781 tgtagcttta gatataaaca atataggtga ttactcacta agtgagattt tcaaaatgta
      841 tatggattta tggtatttta gcatggctac atttagtaca gtaggatatg gagatatggt
      901 tgctacaagt ttgattggga aagcattggc aggtatagaa gtattttttg gagttacaat
      961 aggagcaatt tgggcatctg tgattattaa aagaatgata aggtaatatt tgtgtaaata
     1021 tggataggta ttaattacta gatataaatt ggaccaaata gggaaaattt aaatataaat
     1081 atacttaata atccaagaag gtgaaaaatt tgaaaaatgc actaaaaaaa attatatcat
     1141 tagccatgat tctagttttt ataataccaa attattcata tgcagatggt aaatctgtag
     1201 acaagtattc aaaaacatct atacttatag accaagaaac aggtagagtt ttatatagta
     1261 aagaacctga tatgaaggta ccattagcta gtttaagtaa gatgatgact tttttacttg
     1321 caatagaagc tattgaaaat aaagaagtaa aagaaactga taaggtaaaa atagacaaat
     1381 ctattgcatc agtaaaaggg tcaagttata aacttaaaga tggtgaagaa gtgccactta
     1441 tagagcttat gagaggtctt atgattgtat caggaaatga tgcggcaatt gctatagcaa
     1501 aacatatttg taaaacgaaa gaagcatttg tagatagaat gaataaaaaa gcaaaagaaa
     1561 taggaatgag taatacacat tttctaaatc caaatggtct tccaatatac gatttaagca
     1621 accccaaaaa accagcaaaa gaaaatatct ctactgcaaa agatatagct attcttggaa
     1681 aatacatgtt tgaccattat gaaaaacaag taacagctat aactgatatg gagacatata
     1741 caaattcaga tagaagtttt gaaaaaagca atacaaatgc attattaaga attataccag
     1801 aagtagatgg tataaaaact ggatatactg gcaatgctgg atattgctta tcattttcta
     1861 tggttgtaaa taaaaatgag aaaaatgaga aaaaacgtag agtaataggg gttgtattag
     1921 gtactaacca taaaaataaa agaacctctg cttcattagc tctactgaaa tatggaaaag
     1981 aaaatttcaa tatgaaaaaa gtaattgata aagatagctt tattggtaag aaatatataa
     2041 aaggaatgaa agagttagag gttacattaa aagcaaaaga tgaactttat acaatattaa
     2101 aagatgatga aagtataaaa tcagaagtta agattaaaaa tatagagtac cctgttaaaa
     2161 aaggtgatac tatgggtgtt ataaaatatt atactagttc aggtgactta cttggaagtg
     2221 tagatatagt aagtaataat agtatagata acgtatcatt aaaaacaaaa ttaaaaatga
     2281 aatttgcaaa ttaaattcaa aaaatggtat aaaatacaca aaaaaatata aaaatagaaa
     2341 atatggaaaa aatattgtta tttcttcgcc tctgtgatat actactagag gtgaaaaaat
     2401 aaaataaatc acacacagca ttgggatcct taaaaggtgc ctattacatg gttttttagg
     2461 gagaagatca agctggaggt ataaaaccag gaggtaagat atgtcagtaa tatcaatgaa
     2521 acaattatta gaagcaggtg ttcactttgg tcaccaaaca agaagatgga accctaaaat
     2581 ggctaagtat atattcacag agagaaatgg aatatatata atcgacttac aaaaaacagt
     2641 taaaaaagtt gaagaagctt acaagttcac taaagaagta gctgaaacag gaaagccaat
     2701 cttattcgta ggaactaaaa aacaagctca agatgcgata aaagatgaag ctgaaagatg
     2761 tggaatgtac tttgttaatg aaagatggtt aggtggaatg ttaacaaacc acaaaactat
     2821 aaaaacaaga ataaataaat taagagaatt agaaaaaatg gaagaagaag gcgtatttaa
     2881 tgttcttcct aaaaaagaag ttataaaatt aagagctgaa aaagaaaaac tagaaaaata
     2941 cttaggtgga ataaaagaca tgcctgaatt accaggtgca atgttcgtag ttgacccaag
     3001 aaaagaaaac atagctattc aagaagctca tagattaggt ataccagtag ttggtatagt
     3061 tgatactaac tgtgacccag aacaattaga ctttgctata ccaggaaatg atgacgctat
     3121 aagagccgta aaattaataa ctggtgctat ggcaacagct gtaattgaag gtagacaagg
     3181 tgctgaggaa gaagtagctg aagaccaaga ataataattt gacaattaaa tggtaagggt
     3241 cgcccttacc atttataata tggaatagaa taagtaggag gaaacttaaa aaatggctaa
     3301 tataactgct caaatggtaa aagagttaag agaaagtaca ggcgcaggaa tgatggactg
     3361 caagaaagct ttacaagaag ctgaaggaaa catggaaaaa gcagtagatt tattaagaga
     3421 aaaaggatta tcaaaagctg ctaaaaaagc tggtagagtt gctgctgaag gtctagttgc
     3481 aatagaaatg aatgatgata atacagttgc ttctatggta gaagttaact cagagacaga
     3541 tttcgttgct aaaaacgaag acttcaaagt gttcgttaaa gatgctgctt gtatggcatt
     3601 agctactgat aaagaagata tagcttcttt attaggtgaa actcataaag agggaataac
     3661 tttacaagaa gttttaaata atagagttgc aaaaataggt gagaaattag actttagaag
     3721 atttgctaaa gttgtaacta atggacaagt tgctggatac attcacggtg gtggaaaaat
     3781 aggtgttctt gttgaaatgg aaacagaagc tagagatgct aaagttttag aattaggtaa
     3841 agatgtagct atgcaagttg ctgctatgaa tcctaagtat gtttcaagag atgaagtaga
     3901 tgctgaatac atagcacatg aaacagaagt attaactcaa caagctttaa atgaaggaaa
     3961 accagctaat atagttgaaa aaatggttaa aggaagatta gaaaaagaat taaaagaggt
     4021 ttgtttatta gagcaaactt tcgttaaaaa tccagatata actgttaaac aattagttgc
     4081 tgatgttgct aaggcagtag gttctgatat aaaagttgtt aaagttgtga gattcgaagt
     4141 tggtgaaggt atacaaaaga gagaagaaaa ctttgcagaa gaagttgcta agcaacttaa
     4201 gtaataacca aagagagaac acgcaggtgt tctctttttt aaaaaataga aaaaattgga
     4261 tatgaaggat tgaaatagga ggtttctgat ggataaacca atgtacaaaa gggtattgtt
     4321 aaaattaagt ggagaagcat tagcaggaga aagaggtttt ggtataaata atgatgtagt
     4381 taatgatatt gctattgcta taaaaaaaat acaagaaata ggtgttgaag tagctgtagt
     4441 agtaggtgga ggaaacttct ggagaggcag aaccagtgaa ggaatggata gaactactgc
     4501 tgactatata ggaatgttag ctacagttat gaatgcaatg gcgctacagg atgcactaga
     4561 aaatatagat gttgctacaa gagttcaaac tgctatagac atgagacaaa ttgcagaacc
     4621 atatataaga agaagagcag ttagacactt agaaaaggaa agagttgtta tatttggtgc
     4681 aggaacagga aacccttact ttacaacaga tacaactgct gctttacgtg ctgctgagat
     4741 ggaagcagaa gttatattgc tagctaaaaa tgttgatgca gtatatgaca aggatccaaa
     4801 agtacatgca gatgctaaga aatttacaga attaagttac atggaagtta tacagaaaga
     4861 attaaaagta atggattcta cagctacatc tttatgtatg gataataaaa ttccaataaa
     4921 ggtatttgag cttacaacag agaatataat aagagcagta aaaggcgaaa atataggaac
     4981 tactgtaaaa taatgaagaa tgaaggagga gtaaaattat gaaactggaa atacataagc
     5041 aattagaaga gaaaatgaat gggacaattg atgctttaaa gtttgaattt ggaactataa
     5101 gagcaggaag agctaatgca caaatgctag acaaaataag agttgattat tatggaactc
     5161 caactccaat aaatcaaata ggtgctatat ctgtaccaga accaagaata ttaatgatat
     5221 ctccttggga taaatctgct atgcatgaaa tagaaaaagc gatagctaac tcagacttag
     5281 gattaaatcc atcaaacgat ggtgaggtta taagactttc tgtacctgct ttaacagaag
     5341 aaagaagaaa agaattagct aaaaaagcta gtaaagctgc agaagaattt aaagttagaa
     5401 taagaaatga aagaagagac gccaatgaga aaattaaaaa gatggaaaaa ggcggagaat
     5461 taactgaaga tgaattaaaa aaagcacaag atgaagttca aaaaatgact gataaattta
     5521 taaaagaaat agatacatta ttatctaaaa aagaaaagga cataatggag gtataatatt
     5581 gaaaaaatat tataaccttt taggtcttca tgtagatgag gtaaaacaat attttgatga
     5641 gcaaaatata aattataccg taaaatctat tgaaggtaaa aaggataaag aaaaacttgt
     5701 agttcctaga gttataaaaa tatctgagat aggtgatagt gtagaactga taatcacata
     5761 cttttctgac tccttagttt aaggagtcag aacaatattg gaggcaaaat atgaataaca
     5821 acattatata tgatatagac ttaaataaca tacctactca tattgctatt ataatggatg
     5881 gaaatggaag atgggcaaaa gctagatttc ttccaagaac tgcaggacat aaggctggag
     5941 tagaaactat aagagatata gtaaaagaat gctcaaaatt aggtgttaag cacttaacat
     6001 tatatgcatt ttcaacagaa aactggaaaa gaccgaaact tgaagttgac acacttatga
     6061 acttattatc tacatactta agaaatgaaa tagctgaact tcaccaaaac aatgtaaaag
     6121 taactgctat aggagatata agtgctttac caaaaacttg tataagagaa ttaaactcag
     6181 ctaaggaact gacaaaaaac aatacaggag ttaacttaaa tttagcatta aactacggaa
     6241 gtagagcaga tataaaaaat gctcttatag atatagtaaa aaattgtgaa agtggtaaaa
     6301 tagatataaa caatattgat gaagatatta taaaaaacta tttaagcaca aagtctatac
     6361 cagatccaga cttggtaata agaacaagtg gtgaacaaag attgagtaac tttttacttt
     6421 gggaagttgc ttattctgag ttttacttta ctgatataca ttggcctgat tttaaaaaag
     6481 aagaactaca gaaggctata tacgtatatc aaagaaggga tagaagattt ggaggactta
     6541 agtaaggaga ttattttatg cttacaagaa ttattgcttc actagctctg gttccattat
     6601 ttctatttgt tgtatatgga ggtattcctc tatatatagc tgagacagca atagtttata
     6661 ttgcattgca tgagttttat aaggcattta aaataaaaga tgtacatcca atattcatta
     6721 taggatattt attttctatt tatttagctg taaaaaatat ttttaattta ccattagaat
     6781 atacttatgc agtaattttt attttatttt tagcaagtat aatttatatg ttgatgggca
     6841 aaaacaatgt tatagatgtt tcgattacgt ttttaggagt tttttatata ggagtatttt
     6901 tagattttat aattataact ataaatggat ttgaaaaagg tagtatatat gtatggctta
     6961 tatttgtgat atcttttatg acagatatat ttgcatattt tagtggttat ttacttggaa
     7021 aacataagct aattcctaaa gttagtccta aaaagactat agaaggagct attggaggaa
     7081 taataggaag tacactttgt tgtattttat ttggatattt atttggcatt gatttgcttc
     7141 aattagcgat aataggaagt ataggaagtg taatagctca attaggagat ttatttgcat
     7201 cttctataaa gagatatgta gggataaagg attatggaaa gataatacct ggtcatgggg
     7261 gtatattaga tagatttgat agtgttatat tagttgcacc ttttgtatat agtgcgatta
     7321 aattttttat tagataaaga ttggttggat ttaaaagagc aatctataat tgctagtaat
     7381 gaaaatagct atgtaacttt taaatgcaga gatattctgc agttattagt tacatggcta
     7441 ttttcattgt tcaaaaattt agaagacctt ggggaatttt ataagaataa gtattacttt
     7501 ataatagtat actatatttg tagtgagtat attttttata ctataactat cattaaaaaa
     7561 tatttttttg tttaataatt tcgatatttt tagctttatt atatatttat
//
LOCUS       DAEPOZ010000180         7552 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.180, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000180 DAEPOZ010000000
VERSION     DAEPOZ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7552
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.180"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            172..1149
                     /locus_tag="KR440_002858"
     CDS             172..1149
                     /locus_tag="KR440_002858"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBH0699870.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKTHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            1142..2110
                     /locus_tag="KR440_002859"
     CDS             1142..2110
                     /locus_tag="KR440_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HBH0699871.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLIVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            2294..2884
                     /locus_tag="KR440_002860"
     CDS             2294..2884
                     /locus_tag="KR440_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBH0699872.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            2903..3673
                     /locus_tag="KR440_002861"
     CDS             2903..3673
                     /locus_tag="KR440_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HBH0699873.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            3810..4778
                     /gene="dusB"
                     /locus_tag="KR440_002862"
     CDS             3810..4778
                     /gene="dusB"
                     /locus_tag="KR440_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBH0699874.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            4887..5366
                     /gene="greA"
                     /locus_tag="KR440_002863"
     CDS             4887..5366
                     /gene="greA"
                     /locus_tag="KR440_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBH0699875.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            5381..6910
                     /gene="lysS"
                     /locus_tag="KR440_002864"
     CDS             5381..6910
                     /gene="lysS"
                     /locus_tag="KR440_002864"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBH0699876.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            7219..7413
                     /locus_tag="KR440_002865"
     CDS             7219..7413
                     /locus_tag="KR440_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699877.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NIWADRVRANSYSYENKIAK"
BASE COUNT         3028 a          758 c         1513 g         2253 t
ORIGIN      
        1 tatttaaata aaatgtaaaa ttatataaat catcaatata aaatataaaa caaccattat
       61 aataattaaa aaggaaattt aaaattaata gtattaaaag tatattaata atgtataatt
      121 atttattaga agaaaataag tcatgaaatt atgtcatatt agaggtgaaa tatgcgagat
      181 aaaataatag agattatatt aaataataaa tctgaattta tttcaggaga agaattatca
      241 aaacaattag gggtgtcacg tgcagctata tggaaacata tgaaagcctt aaaagaagag
      301 ggatataata tagaatctgt aaataaaaaa gggtatcgat tagcggaaaa tccaactgat
      361 ttattaagcc cacagaatat ttactataaa ttagatacag agtttattgg aaaaaatatt
      421 gaacatcttg acactataga ttcaacaaat gaacatgcta aaaggatagc tataaatagt
      481 gtagatggga ctgtgatttt aagtgaacat caagatatgg ggaaaggacg attgggaaga
      541 agatgggatt caaaaactca tgaaggaatc tggatgagta ttatattaaa accagatatg
      601 gaaccattca aagctccatt tataacttta atagcaggag caagtgtaac gaaagcttta
      661 aacaatctag gagcaaatac attaataaaa tggccaaatg atgttatttt aaatggaaaa
      721 aaaatatgtg gtatattaac agagttatct gctgaaattg aaagaacaaa ttatgttgtt
      781 cttggaattg gaataaatgt taaaataatg gattttgaac aagaaatagc tgacaaagcc
      841 acatcattgt ataaagaagg gtatatgtta tccagagtag atattgttag gaatgtattg
      901 actgaatttg aaaaattata tttagattat actgtaaata attccaaaga aaaaacctta
      961 aaaatatgta gagattattc tgcaattata gataaaaata tatatgtatt aaaaggagat
     1021 aatagagaac tagtgaaatg tttagacata aatgaagagg gaaatttatt agttcaaaga
     1081 agtgatggaa gtataaaaga aattatttca ggtgaagttt ctataagagg agaaaaagga
     1141 tatgtataat aatattttgg atatagaagg cttaaaggtt gggcaggttg aggatagaaa
     1201 tggtcttact gggtgcacag tagtaatatg tgaagaagga gctacttgcg gtgttgatgt
     1261 aaggggttct gctccaggga ctagagaaac cgatttatta gaccctgtaa atatggttca
     1321 aaaagttcat gcagtagttt tggctggagg ttctgcatat ggtcttgaat ccacttgtgg
     1381 tgtaagtaga tacttagaag aaaaaggaat tggctttgat gtaggtgtag gtaaagtgcc
     1441 tatagttgta ggagcagttt tatttgattt aggagtaggg agctttaaat gtagaccaga
     1501 tttaaagatg ggttatagag cttgtgaagt tgcaaatact gatattttaa atcaaggaaa
     1561 ttatggagca ggttgtggag ccactgttgg aaaaataaga ggtcaggaat atgctatgaa
     1621 aggcggaata ggtagctatt ctataaaatt agataatggt ctaattgtat ctgcattaat
     1681 tgctgtaaat gctcttggag atgtttatga aaatggtaag gttatagctg gtgctttaaa
     1741 tgataaaaag acagaaacaa ttgatacata taaattaatg aaacaaggtg aaagtaaagg
     1801 tggatttagt gtagataata ctacaattgg gataattgca acaaatgcta agttaaccaa
     1861 agctggatgt aaaaagatat ctcaaatggg acatgatgga tatgctaagt ctatttttcc
     1921 tatccataca cctcatgatg gagatacaat attcacaata tcaacaggag aaatagaagc
     1981 agatataaca ttagtaggtt cattggctgt agaggttgtg gaaaaaagta taataaatgc
     2041 agtaaaaaaa gcggatagtg tagaaaatat aatggcatat aaagatatag taaaaaaacg
     2101 agaaatatga tgaaattagc atatttttct tgtcaaattg ttgacaatgg caactcatct
     2161 tgataaaatt attcttgtta gagcgggcat cctacaatgg tagcttgact catagttata
     2221 agaaaaaaac gaaaaaaatt aaaaaattaa gtagtccggc atcttaatga gctggaacgg
     2281 aggtgtattt tttatgatga acacaagtgc atcagtaaga aaagtaaatg taagaaagat
     2341 gactataata ggagtactat cagctatttc tattatgcta tctatgactc cattgggatt
     2401 tattccagtt ggacctacaa aagcaactat aatgcacata ccagttatta ttggagctat
     2461 tatggaagga ccaatagttg gggcagcaat aggatttata tttgggatat caagtctctt
     2521 aaatgctata acaaacccaa cagtaacatc ttttgttttt ataaatccat tagtttcaat
     2581 attaccaaga gttatgatag gtgtattagc ttattatgta tatcagttaa taattaaggc
     2641 tacaaataag gtatacatat caggtttaat tacaggggca ataggttctc tgttaaatac
     2701 agctggagtt ttaggaatga tatatgtctt atatgctgac aagtatcttc atgcaatggg
     2761 taaaagtggt tcagcaggaa aagtaataat ggctcttgcc gcagcaaatg gggtgccaga
     2821 agcaatagta ggggctttag tagtagctgc tgttgccact gtacttaaga aaagcaaaaa
     2881 atagaaagaa ggttaatgac atatgcttct agtatttgat gttggaaata ctaatatggt
     2941 tttaggtata tataaaggtg acaaattagt taattactgg agaattaaaa cagataggga
     3001 aaaaacgtct gatgaatatg gaatcctgat aagtaaccta tttgattatg ataatgtgaa
     3061 tataagtgat attgatgatg ttataatatc atctgtagtt ccaaatgtca tgcattctct
     3121 tgaaaacttt tgtataaagt actgtaaaaa acagccatta atagtaggtc caggcataaa
     3181 aacaggtcta aatataaaat atgataatcc aaaacaagtt ggggcagata gaatagttaa
     3241 tgctgtagca gggatagaaa agtatggagc accaagtata cttgttgatt ttggaacagc
     3301 aactacattt tgtgctatct ctgaaaaagg tgaatatttg ggtggaacaa tagcaccagg
     3361 aataaaaata tctagtgagg cgttatttca aagtgcgtct aaattaccta gagtagaatt
     3421 agctaagcca ggtatgacta tttgtaagag tactgtatca gccatgcaat ctggaataat
     3481 ttatggatat gttggtttag ttgacaaaat aataagtatg atgaagaaag aattgaattg
     3541 tgatgatgtt aaggttatag ctacaggtgg attagctaaa ctgattgctt cagagacgaa
     3601 aagtatagat tatgtagatg gttttttaac actagaagga ttgagaataa tatatgaaaa
     3661 aaaccaagaa taattaatat acagggaatt gggttatata tccaattccc tttttatgat
     3721 agatttctag ctatatgtgg tatattataa tgaggaaata agaatttaaa gttaaatcac
     3781 aaaataaagg caaaaatagg agaaaaacaa tgaaaatagg aaatttagaa ttgaaaaata
     3841 aagtttttct atcacctatg gcaggtgtaa cagaccttcc atttagatta atttgtaaag
     3901 aacagggatg tgggctttta tatacagaaa tgataaatgg taaggcactt tgctatgatg
     3961 atgaaaatac taaaaaaatg cttaagatag aagaggaaga acatcctgta gcagttcaga
     4021 tattcggttc agaaccagag tttatgggaa gagcagcaga gataatgaat gattattcaa
     4081 atgagattct cgatataaat atggggtgtc cagctccaaa agtagttaaa aatggagatg
     4141 gttcagcact tatgaaaaat cctaagcttg cagaagaggt attgagggca gtagtgaaga
     4201 actcaaaaaa gccagtgact ttaaagattc gaaaaggctg ggatgataat agtgtaaatg
     4261 ctgttgaaat agcaaagata gcagaggatt gtggaataag cgctttagca atacatggaa
     4321 gaactagaga acagttttat acaggcaaag cagactggga tataattgca gagattaaga
     4381 aaaatttgag tatacctgtt ataggaaatg gtgatgtatt tacaatagaa gattcaataa
     4441 atatgttaga caaaactggt tgtgatgcta taatgatagg taggggtgct caaggtaacc
     4501 cttggatatt taaaagaata aaccattata tgaatacagg cgagattttg ccagagccaa
     4561 ctcttaacga aaaaataagt actgccatta agcatttaaa acttgcagta gaagaacatg
     4621 gagagtatgt agctgtaagg gaaatgagaa aacacatcgc ttggtattta aaaggactta
     4681 gaaactctgc aaaattaagg gatgaaatta acaaaataga agactatcaa gaagttgtgt
     4741 cgaaattgga gtactatatg gaagactcct tgacataaaa ataatagtga cttataatat
     4801 agcgcataaa ttaaaaaaag attataaaat ttttaaaata taattaggaa aattcctaag
     4861 aaatttgtga aggagttgta tttataatgg aagaaaataa agagttttta ttgacacaag
     4921 agggatatga taaattagaa gaagaattag aaaacttaaa ggttgtaaag agaaaagaag
     4981 ttgcagagag aataaaagtt gctatatctt ttggtgattt atctgaaaat gctgagtatg
     5041 atgaagctaa aaaggaacaa gcacaagttg aagaaagaat tttaaaatta gaaaatatgg
     5101 ttagaaaagc tgttatcata gatgaaagca aaatagacct taatgttgtt acaataggat
     5161 caatagttaa agttaaggat ttagaatttg atgaagaggt tgaatatact atagttggtt
     5221 cgacagaagc agacccatat gatggtaaaa tatcaaacga atctcctgta gggaaagcac
     5281 ttttaggtag agctgcgaaa gaagttgtgg aagtgcaagt tcctgatgga gttgctaagt
     5341 ttgaaatatt agaaataaga agataatttg gaggaaaaaa atgaaaaata atcaacaaag
     5401 caatgaagaa gcgcaaattc aagaagacct tagtgaagta cttcaagtaa gaagagataa
     5461 actaaaaaaa ttacaagagt caggtagaga tccttttaaa gaaagtagat atgatagaac
     5521 tcattattct atggatataa aagataactt tgattctctt gaaggtaaaa ctacaaaaat
     5581 agcaggacgt ataatgagca agagaataca agggaaagct ggctttatag atattcaaga
     5641 ccaagaggga agaatccaat cttatgtaag actagatgct ataggagagg aagaatatag
     5701 tgtattttca acatatgata ttggagatat agttggaatt gaaggagaaa tctttaaaac
     5761 taaaaaaggt gaaatatctg taaaggctaa atctgtagta ttactatgta aatctttaca
     5821 agttttacca gaaaaatatc atggattaaa agaccaagaa ctaagatata gacaaagata
     5881 tgttgactta atagtaaatc cagaagtaaa aaatgctttt ttaattagaa caaaagcatt
     5941 aaaagcatta agagcatact tagatgatag aggattttta gaagttgaga ctccaatatt
     6001 aaatactata gcaggtgggg caaatgctag accatttata actaatcata atactttaca
     6061 tataccaatg tatttaagaa tagcaaatga attgtattta aaaagactta ttgttggtgg
     6121 atttgataaa gtttatgaaa tgggaagaat gtttagaaat gaaggtatgg atttaaaaca
     6181 taatcctgaa tatactgcaa tagaattata tcaagcatat gcagactata ctgatatgat
     6241 ggaaataaca gaaaatgtta ttgcacatat ggctgaagtt gctacaggaa gtatgatagt
     6301 taactatcaa ggaacagaaa taaactttac accaccttgg aaaagaatga gtatggaaga
     6361 ttgtgttaaa gaatattctg gtgtagattt ttctactata aatacagatg aagaggcact
     6421 agaagttgct agagaaaaag gtatagagat aaaacctgga atgagaagag gagaagtaat
     6481 aaatgctttc tttgaagaat ttggagaaga taaattaatc caaccaacat ttataactca
     6541 tcatccagtt gaagtatctc cattatctaa gagaaatgtt gaagacccaa gaagaactga
     6601 tagatttgag gcatttgcta ataagtggga gttagctaat gcattttctg aacttaatga
     6661 ccctattgac caaaaaggaa gatttataga ccaattgaga aaaagagaat taggtgatga
     6721 tgaagctttt gaaatggatg aagatttctt aaaagcatta gaggtaggtc tacctcctac
     6781 aggaggacta ggaataggta tagatagagt aattatgtta ttaactaact ctccatctat
     6841 aagagatgta ttattattcc cgactatgaa acctattgat aacaatccaa ataaagaaga
     6901 agaaaactaa gaaatattag cattctacta agttttaaat aaagtttagc tattaaatta
     6961 gcaataaata gctaaatttt gtttaaaatg tcagtgtaga atgcttttta tatgcttcaa
     7021 agattctttt agatatttaa tttataaatt accaacaaac gttagaaatt gatattagtt
     7081 tttgttgatt gagactttat atttagaaaa tatgattgaa agtaatttat tatgaaaata
     7141 tttagaataa tttatgagaa ggtaaaaaga ataatttatg agaatactat atataaaaaa
     7201 ttattggggg aatgtctgat gtttcaagta attaattttt tatgtttatt ttgtgttgga
     7261 tttttggttc ttgatgttat gatatatttt tgtaaggatg caatggatat ttttgagggt
     7321 acaactaatg ttaataaagt taagagtata aatatttggg cagatagagt gagagctaat
     7381 tcatattctt atgaaaataa aattgcaaaa taggtaaaat tatatatgca tgtattgttt
     7441 gtaatatcaa gggttatatc tattagtttt aattatattt attaaaaaaa ccaaaagtta
     7501 taaccttttt taaaataaaa aacaaaaaaa gtattgacga aatattttta ag
//
LOCUS       DAEPOZ010000181         7551 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.181, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000181 DAEPOZ010000000
VERSION     DAEPOZ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7551
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.181"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            634..1143
                     /locus_tag="KR440_002866"
     CDS             634..1143
                     /locus_tag="KR440_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBH0699878.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            1171..1365
                     /gene="rpmI"
                     /locus_tag="KR440_002867"
     CDS             1171..1365
                     /gene="rpmI"
                     /locus_tag="KR440_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBH0699879.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            1421..1777
                     /gene="rplT"
                     /locus_tag="KR440_002868"
     CDS             1421..1777
                     /gene="rplT"
                     /locus_tag="KR440_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBH0699880.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGIEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            2087..2632
                     /locus_tag="KR440_002869"
     CDS             2087..2632
                     /locus_tag="KR440_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699881.1"
                     /translation="MNEKLMDCFVNPIKCKLLLEVFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKEGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKEDEGK"
     gene            2619..2792
                     /locus_tag="KR440_002870"
     CDS             2619..2792
                     /locus_tag="KR440_002870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699882.1"
                     /translation="MKGNKSDLHEVEPIYTENRFMIYICIQSKVSLHGLYFIVPFHPM
                     TWSGTMKFFNFSY"
     gene            3068..4993
                     /locus_tag="KR440_002871"
     CDS             3068..4993
                     /locus_tag="KR440_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBH0699883.1"
                     /translation="MKIKFVIMPLVIFLIVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFISNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWIINDKFSKTIEVE"
     gene            5394..7298
                     /locus_tag="KR440_002872"
     CDS             5394..7298
                     /locus_tag="KR440_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBH0699884.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFKQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVKVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEGKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSSSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT         3183 a          754 c         1384 g         2230 t
ORIGIN      
        1 attaagtatt tattaagtat tgaataaaat attacaaatt tagttgaaat gaaaatgata
       61 aaaaatgttg tgctatattt tacaaatcat ataaatagag aaattgttgt agcacaaagt
      121 aataggtagt atattagaat ataatatagg atttgttgtg tagtatagta gcatattaga
      181 atataatata ggatttgtta tgtagtatag tagcatatta gaatataata taggatttgt
      241 tatgtatata gtagcatatt agaatataat gtaggatttg ttatgtagaa tatatagagt
      301 catgtagaat ataaatatat aaaaggattt tgggtataaa tttaaaaatt aatagcaaaa
      361 tgagtagtat agacatataa ttagtagtag gaagtaatta aatttttcct aaacaaaaaa
      421 aatagttgca aataatttga ttaagtgata tagtaaataa ggtaagaata aaacaaagaa
      481 gaagtccgct tctcacctta cgccggaggt tagtaaggta aatataagtt aaagcttaat
      541 tttaatttaa gtttgtttaa atttggtaac ttatattgcg gatgattacg tcatccgctt
      601 tttattttat aaaaattttg gaggtgtcct acaattagca aagaattagc aatcaatgaa
      661 caaataaaag ataaggaaat aagagtcctt tcaccaacag gtgaacaact tggagtaatg
      721 cctactaaag aagctcaagc aatggctaat agcaaaaact tagatttggt tcaaatctcg
      781 ccgaatgcga atccaccagt atgtaagata atggattatg gaaagtttag atatgagcaa
      841 gcgagaaaag agaaagaagc taagaaaaaa caaaaaacta tagttgtaaa agaagtaaga
      901 cttagaccag gtattgaaca aaatgattta aatacgaaag ctaataatgc tataaagttc
      961 cttaaaaaag gggataaagt caaagttgaa cttagattta gaggaagaga attaggacat
     1021 aaagacatag gtaaagaagt tatgcttaag tttttagata taataaaaga gtttggagaa
     1081 ccaacaaaag cccctgcatt tgagggtaat aatatggttg taattataga tccaaaaaaa
     1141 tagatgatta actgagagga ggaatttgtc atgcctaaaa tgaaaactca tagaggtgca
     1201 gcaaagagat taaagaaaac aggaactgga aagttaaaga gagctaaagc ttttaaaaaa
     1261 catatattaa ctaaaaaatc tgcaaaaact aaaatgaact taagaaaatc aactttagta
     1321 agtgatggag atgccaagag aatagcacaa ttattaccat actaagatac agattgtaaa
     1381 taataactat agaagaggga tataaaagga ggtcataacg atggcaagag ttaaaaaagc
     1441 tatgaacgct cgtaaaaagc ataagaaaat attaaaactt gcaaaaggat tcagaggatc
     1501 tagaagcaaa ttatatagac cagcaaatac attcgtaatg aaagcattaa aaaatgctta
     1561 tataggaaga aagttaaaga agagagactt cagaaaactt tggatacaaa gaataaatgc
     1621 agctgctaga atgaatggaa tatcttattc aagattaatg aatggattaa aattatctgg
     1681 tattgaagtt aacagaaaaa tgttatctga aatggctata caagacccag aaggatttgc
     1741 aaaattagca gaagttgcta aagcaaaatt agcttagtat tgaatcatga gatgtaaagt
     1801 ctttgagtta tataaataat tcaaggactt ttttattttt tgaaaaatac ttattagtgt
     1861 catgtacaac caattttata atgataaata aaataagcaa tacaatagaa ttttatgaga
     1921 tttattataa gaattgttac aattatttat catagctata aaaagtcata gtaaaaatga
     1981 tattgatgta tgtttatata tgtataaagc tttcaatcaa tcttgaatta atattatcaa
     2041 catgataata ttatcttatt aataatataa ataaggggga gataatatga atgagaaact
     2101 aatggattgt tttgtaaacc caattaaatg caagttattg cttgaagttt tttcaaataa
     2161 tcgaactaca gctaaacaat taggagaaaa atttcaagat atatcaaagg caacacttta
     2221 tcgttacctt aataaaatgt tgaaagatga tattataaaa gtggttgaag aaaataaaat
     2281 aaggggaact gttgaaaaag tatatgctat gaagttagat atagctgatg atgtgaacaa
     2341 aattatagat gagaactctg gagaagaata tgctcaactg tttactcaat atatgatggg
     2401 atttttaaga gaattttatg aatacgctga aagaaaggaa aaggatatcc taaaagaggg
     2461 ttcaggattt tctgtctgtc cagtatatgc tagtattgat gaaatagttg aagtgagtaa
     2521 aaaaattgct agtatactag aacctctaag aaataactcg cacgaagaag gcagaagaat
     2581 gcattctata ggagtgataa tcactccacc taaagaggat gaagggaaat aaatctgatt
     2641 tacatgaggt agagccaatt tacacggaaa atcgttttat gatttacata tgtatccaga
     2701 gcaaagtatc tctgcatgga ttgtacttca ttgtaccatt tcatccaatg acttggagtg
     2761 gaacaatgaa gttttttaat ttttcatatt aatgcctaaa gaaatgaact ttagaccaca
     2821 tatgcaatta ggctacatta tacaattaga ccagatatac agaataaaaa ttagtatttt
     2881 tatagcagat aaattgactg ttattcttct taaaaatcat ctattagtta ttgttagcat
     2941 attgaaagaa attatagtaa tccattgaat aaatgaatat tgcccataat catataaact
     3001 gctatagttt aaaataaagc aggacgttaa attttatagt aatcttaaaa tttaagaggg
     3061 ggattttatg aaaataaaat ttgttattat gccattggtg atatttttaa tagtattatt
     3121 tgtggggtgt caaagtacaa aagatacaga agttaaagca aaagatacag aaattaaaat
     3181 attaggtaca ggtgatttgc attcaatttt gacagactct atggtttcat atgtaaatga
     3241 ggaaagggaa aagaataaaa atctgcttat ggtagatgcg ggagatttct atggtacaca
     3301 aagtagagaa atgtgggaat ggtcttctgg aaaaaagtta attaatatta aaagaaatgg
     3361 tagagcagag tatgatgaca taattaaaag tagtaaagat gaagtaccta tagttaaaga
     3421 tatggcaaag ttgaaatatg atgctgtagt tttgggagat aatgaattta tttcaaatga
     3481 taagcaaagt cttgataaac tagtttctga ttttaaaaat aataatatgc cattggtatc
     3541 agcaaatata tatgaacaat ctggagaaaa ttatgtacaa ccatatgtaa tgaaaaatat
     3601 taagacagat gagggcaatg taaaagttgg aatactaggt cttactataa aagaagtagg
     3661 ggaaaccctt ggtcttgagg attttgaaaa ggataaaaaa gctagagagc ttggagaaca
     3721 agctgattac aaaggaaaat tatatgcaaa tgatttagta gaagatgcaa agaaatggat
     3781 aaaagttatg gaaaaagaaa gtccagacat aatagttgca gttgtacact caggggaaga
     3841 acctaaaaca gatagaaatc caggaaatag aataaaagag ttagcaacta cagtggatgg
     3901 aatagatgca attgtagctg gtcatactca tgaaaaaata gatgaacaca aatataaaaa
     3961 taaatctgga gatgaagtaa ttgttacaca accaggttgg catggagata gagtatcaga
     4021 gattaatttc aaattaaata aagagaatgg aaagtgggac ataaaggaaa agtctagtaa
     4081 attaaaaata attgataaag aagtaagtat agttgctact gcagatttac attcacattt
     4141 ctctgataaa ttaacagttg accttgttaa tgaacgtagt aacccaattg aacctgttgt
     4201 agtagatgca ggcgacttct tagaccctca aacagatgaa atgactcaat ggtataaaga
     4261 gtggaaaagt ataagagata ataatagtga taaaacgatt agtagatgcc caatggtcat
     4321 ggatatgaat cagggaatgt atgatgcagt agtacttgga aatcatgaat ttgtatctga
     4381 aaaagatgaa ttttggtcag atgaacgcct tttagatgct gttatagagg attttgaaca
     4441 ggtggcaatt tctgtgttat cagcaaatat atataggcaa tctggtaaaa attatgttaa
     4501 accatatatt ataaaagatg tggagacgag tgaaggtaat gtaaaagtag gaatattggg
     4561 acttactata aaagaagtag gtaaaggtct taaaaatgta aacttacaag agcaatttca
     4621 atataaggat aaattgtatg caaatgattt agtggaagat gcaaagaaat gggtaaaagt
     4681 tatgaaaaag aaaaaaccag atataatagt tgcagttgta cattcaggag aagaaccaaa
     4741 aaatccaaaa cacccaggaa atagaataaa agagctagca actacagtag atggtattga
     4801 tgcaattgtt gctagtcata ctcatgaaaa aatagatgag catgaatata aaaataagtc
     4861 tggagaaaaa gttatagtga cacaaccagg tgagcatggt aaatattatt ctaaaattac
     4921 ctttaaagta aataaagaaa agggttcgtg gattattaat gataaattca gtaaaacaat
     4981 tgaggttgaa taactttata aaatttatag ataaagtaaa ttatctgata ttgacatgat
     5041 atataagata aagtttctaa tattagtata atgcataatg tgaaatattt taataataag
     5101 agaataaaca agacaaataa aaatatttta acatgtaaga tatagtatga actattatac
     5161 taatgcttac tttataattt gaacatgtta gaataagtct tatttgaaaa ttttagtgta
     5221 ttagagtaag tattatttta ccagtaaaaa atattgagaa ctatatgtag atttgcttaa
     5281 aaaggaattt acatatagtt cttttcgtat aataaatagt acaatcttat atggtaaaga
     5341 taaagtgtga tattaaattt tattatcatc caaaatctaa ggaggagtct cttatgaaaa
     5401 taaaatttat tatagtagca tcaataatat ttttaatagt attacttgtg gggtgtcaaa
     5461 gtactaaaga cacagaaatc aatgtactcg ccacaagtga tttacattca attattccag
     5521 ataatttaat ttcctatgta gaagaggaga gaaaatatga tgagaatcta cttatggtag
     5581 atgcaggaga tttttttgac atgcaaagtg gtgagatgaa taagtggttt actggtcaaa
     5641 aattagtaag atttaaagat gggataccag agtacaaaaa aatagctgaa ccacgtgaag
     5701 gagaagttcc attggctaaa aaaatggcaa agctgaagta tgatgcagtt acatttggga
     5761 atcatgaatt tgtatctaat gacaagcaga gccttgataa attggtttct gattttaaaa
     5821 ataacaatat accattagta tcagcaaacg tatatgaaca atctggagaa aactacgtaa
     5881 agccatatgt aatgaaaaat ataaatactg acaagggaac tgtaaaggtt ggtatacttg
     5941 gtctgactat aaaagaagta ggggaacgtt caaggtttaa aagttttaaa caagataaaa
     6001 aggctagaga acttggggaa caacctcaat acaaagggaa attatatgca aatgatttag
     6061 tagaagatgc aaataagtgg gtaaaggtta tggaaaaaaa agaaaagcca gatataatag
     6121 ttgccattgt acattcaggt gaagagccaa aaaatcctaa aaatccagga aatagaataa
     6181 aagaactagc aacaactgta gagggaatag atgctattgt agcaggtcat acacatgaaa
     6241 aaatagaaca gcatacatat aaaaataatt ctggtgaaga agtaatagta acacagcctg
     6301 gagctcatgg taatagtata tcaaagatta attttaagtt ggataagaag aatgataagt
     6361 ggtttataaa agacaaatat agtgaattaa aggtttttgg aaaggaagta aatataatta
     6421 caacttctgg tctgaatcca aaattttcag atgaaatggc agttaatttg tttaatgaaa
     6481 gagatagaga tgttcaacct atctttatag atactggaga ttttttaaat actaatacaa
     6541 aagaaatgac taagtgggct aaagagtggc aatacaatga aaataaaggc atctataaaa
     6601 aaataaatga atggccaatt gtatatcttg gttatgatgc tgtagtactt ggtagccatg
     6661 aatttacaga ggataaagag acttttgagg gaaaaaaatc tacattggat tatattatag
     6721 aaaactttga agaaatgaag ataccagtat tatcagctaa tatatatgat gaatctggag
     6781 aaaattatgt aaaaccttat ataattcata atgtagaaac tagggaagga aatataaagg
     6841 ttggagtatt aggtcttact actactgata aggatgaaga agacttaagt agctcaagct
     6901 taagcaaaga ctcaaagact tcgaaaattc ctgaagaaac aggaaagtta tatactaata
     6961 atctagtaaa agatgcaagc gaatgggtaa aagttatgca aaaggaaagt cctgatgtga
     7021 ttgttgcagt tgtacattca gataataata gtactctaaa aaatccaagt agtgaagtca
     7081 aaaaacttgc aatgtcagta gatggaatag atgctattgt tgtaggtcat acgaaaaata
     7141 aggtagaaga acatatttac aaaaataagt ctggagatga ggttatagta acacaatctg
     7201 gagaagattg ttattcaaaa ataagtcttg aactaagaaa agaaaatggc aagtggaata
     7261 tcataaacaa atatagtaaa gcaataaaaa tgaagtgaat ttattgatat ataaaccatt
     7321 attaacactg atttttataa tataataaat actgattcta caatataatt aacactactt
     7381 ttttaaatat aataaatact gattctacaa tattattaat actacttttt ataatataat
     7441 caatactact ttttacaata taatcaatac tgattctata atataattaa cactactttt
     7501 taaatataat caatactgat tctataatat taacactatt ttttatgcta t
//
LOCUS       DAEPOZ010000182         7518 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.182, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000182 DAEPOZ010000000
VERSION     DAEPOZ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7518
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.182"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..360)
                     /locus_tag="KR440_002873"
     CDS             complement(<1..360)
                     /locus_tag="KR440_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH0699885.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKE"
     gene            928..1290
                     /locus_tag="KR440_002874"
     CDS             928..1290
                     /locus_tag="KR440_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699886.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSRIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            1274..1990
                     /locus_tag="KR440_002875"
     CDS             1274..1990
                     /locus_tag="KR440_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699887.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDVSNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEILNQFKNESENIRNQEVVEFTEEDEEYEEELKQNIIE
                     NIKKNFDEKKLVQVYKYRGV"
     gene            2010..2429
                     /locus_tag="KR440_002876"
     CDS             2010..2429
                     /locus_tag="KR440_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699888.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            2419..2943
                     /locus_tag="KR440_002877"
     CDS             2419..2943
                     /locus_tag="KR440_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699889.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYVDENNNEESISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVLITNNGQYVNIKLKLSKRSQKYE
                     TDFKIKVWNFSESI"
     gene            3255..3812
                     /gene="efp"
                     /locus_tag="KR440_002878"
     CDS             3255..3812
                     /gene="efp"
                     /locus_tag="KR440_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBH0699890.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            3904..4359
                     /locus_tag="KR440_002879"
     CDS             3904..4359
                     /locus_tag="KR440_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBH0699891.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCPSCDDRE"
     gene            4522..5232
                     /locus_tag="KR440_002880"
     CDS             4522..5232
                     /locus_tag="KR440_002880"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBH0699892.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            5286..6356
                     /locus_tag="KR440_002881"
     CDS             5286..6356
                     /locus_tag="KR440_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH0699893.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            6384..>7518
                     /locus_tag="KR440_002882"
     CDS             6384..>7518
                     /locus_tag="KR440_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0699894.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKILEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEINDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEASKDKQKIKLESLN"
BASE COUNT         3187 a          639 c         1348 g         2344 t
ORIGIN      
        1 ttctttatct gttccgatgg ggtcttgtag agatacttga gttttatttt ttttgttaga
       61 tctaatattc attaaaatct cattttcaat acatctagaa gcatatgttg ctaatcgagt
      121 tcccttatct atgttataag tttctatagc tttaattaat cctattgtac ctatggatat
      181 taaatcatcc tgatcttccg ttgaattatt atatttcttt gctatataag ctactaatct
      241 catgtttctt tcaattaatg tatcttttgc acttttatcg ttttctattt taaatttagt
      301 taaatactca atctcttctt caggggttaa gggtttttca aaagatttaa gagctgccaa
      361 taggtcaccc ccttttcaac ggtcacttta aattatatga aggagactta tccaagttga
      421 ctgttatcta ataaaataat atgcatattt ttagaattta aaacatatat ttgcaaactt
      481 ttgaatggaa tatgacataa agagtgggaa tttaaattta agtttttgtt gtatatatat
      541 gaaacaaggc gaagaaatta ttgatttata aagtataaat tggaattgag gtgtcaataa
      601 atataaaagg aaatagtatt tatatatgtt ttggtgttga ggtaaaagat taatgcaaag
      661 cattagagtc tataaaaata tgaatatagg aggtgtaatt ttacaatact taaaaaagta
      721 aatgtaatta attgtttgta taaagttgat tacaagcaaa aagtatcagt catagttaat
      781 tctgttaagg atgaaggtgg tgtgttaatt tatatatgta tgggaagaaa ttttgattac
      841 aatgaaaaaa cttacaaaag atatttatga aaaaacttat agttaaatgt ttatatataa
      901 aatattattt aggggttgtt tttattattg aaaataagaa agagtggttt tatatcaatc
      961 gaatgtataa taagtattgc tatattatat gtggctgttt atttagtttc tacatcattg
     1021 tataattgtt atagttttat cagtagaaat atatctgaca gagaaatgtt aagtacagca
     1081 aaaaaatata tagaagatga gaagtataga atacaaaata gtaagtatga gttaattgaa
     1141 gataagatag aaaaaaatta cataaatgga tatgaaatta acagtagaat agagcaaatt
     1201 ttagattatt atcaatgcta tgaaataaat atagagataa aaaatgaatt taaaaaactg
     1261 aggtttaata gctatgttac tagaaaataa aggctctata ttaataataa cattgataat
     1321 attttctatt ataagtactg tgtgcattat gtgtataggt ttaatttata cacataataa
     1381 tatgtttgag ttaggataca aggacattca actaaaagaa atttctttaa gtggaataga
     1441 aatatcagta agtaatgtac tttcttgtgt tgaggaagct attgatgtat ctaataatga
     1501 agacgaattt agagaatatt tcttaggaaa taatatgatt gaatgtataa aaaaaataaa
     1561 agatacatct tatagtagtt tagaagaact ttctttaaaa gtagaaaata atactattta
     1621 tgacagagga gatttttttg aatttgatat agaaagtaat gttagagaaa aagaattttt
     1681 aaaagcaata aaagtaagag tgaaaatcaa aaatccatgg ataggaattg acattgaaga
     1741 ttatatggat aaatctaata acaataaaat tgatgacaaa acattcaata attataaagc
     1801 atctgaaaat aatgaaattc taaatcaatt taaaaatgaa agtgaaaata taaggaatca
     1861 agaggttgtt gaatttacag aagaagatga agaatatgaa gaagaactta aacaaaacat
     1921 aattgaaaat ataaagaaga attttgatga aaaaaaacta gtacaggtat ataagtatag
     1981 gggtgtataa ctttgaaaaa agaaggaatg tgactgtaat agaattaata gtatctatca
     2041 gtataatact cttgatagtt acattatctt ttccaaaaga taatcttgaa aatcataaaa
     2101 taaatttatt tgcacgtcaa ctttgttcag atataaggta tgttaggcga attaatatgt
     2161 ttagcaacta caatacatat atatattata taaatcaagg taaacacgaa ggttatgtat
     2221 tgagaaaaga tggtaaaaac attaaatcaa taatgctacc taaaaatgca aaaatagcta
     2281 gaagtattga aataataaag tttaaatctg atgggtcgcc tcaaaaggcg tctacaatag
     2341 agatatataa taatagatta agaaaaacta taacaataac acctgtaagt ggaagagtac
     2401 ttctgaaaga gggaaaatat gaaacataaa tatggttact tgcttttgga aagtgtagta
     2461 tcattgtctt caatggtgat aattatacta gttttatatt ctatattttt gtctacaata
     2521 aacttaaagt taaaagtaga agataagata gaactacaac aacaaagtct tgaaatcata
     2581 aaatctatgg aaggaattat tagcaattct atgggaataa tgaatgtatc taattatgaa
     2641 gaaacattta aaaagacaac gtctataaaa tgtagatatg tagatgaaaa taataatgaa
     2701 gaaagcataa gtaataaaga aattatattg aatgaaagac gaaataagtt atttgtaaat
     2761 tctttaaatg gcgaaagtag tcaagcaggt ggttatgaaa ttggagatta tgtagatgag
     2821 atgtatgtat taattactaa taatggtcaa tatgttaata ttaaattgaa attatcaaaa
     2881 cgtagtcaaa aatatgagac tgatttcaaa ataaaagtat ggaattttag tgaaagtata
     2941 taaaaataca taaatttaag ataaagaaat gacatcaata agtcatataa aatataatta
     3001 ttgagaacta tataactaaa taatatcaat atgtaaagtg tatagattaa gaatatagag
     3061 gataataata aaaatataga ataagtaatt tgccaaatac ttgtttatat tgtataactt
     3121 atgatattat gatattagta aaaatttgta atcctgactt gacagaattc aagtaagaga
     3181 tataatttta aaggcatagt aatgtaaaat ttactaaaaa ataagaaatt tattttaata
     3241 tggaggaaca aaaaatggta tcagcaggtg attttagaaa aggtgttaca tttgaaaaag
     3301 atggacaacc atgtttagta gttgattttc aacacgttaa gccaggtaaa ggagctgctt
     3361 tcgttagaac taaatacaaa aacttaaaaa caggagctat aagagaagaa agtttcaatc
     3421 caagtgaaaa attccctaag gctgttatag atacaagaca aatgcaatat ctatacaatg
     3481 atggtgagtt atattatttt atggatcaag aaaattttga acaaatacca ttaaactatg
     3541 agcaagttga agatgcaatt aagttcttaa aagaaaatga agttgctaca ataagatttt
     3601 accaaggaca accattccaa gtggaagcgc caaactttgc agagctagaa gttacagata
     3661 cagagccagg tataaaaggt gatacagcaa gtaatgtaac taaagcagct acagttgaaa
     3721 ctggagcagt tgttcaagtt ccattattta taaatactgg agataaagta aagatagata
     3781 ctagaacagg tgaatattta tcaagagtat aaaaatttat gtatataaaa gcttatatgt
     3841 cttaataata taagatatat gagcttttag tttatatata aaaaagaaag cgaggggtgt
     3901 tacatgaaac atttatatga agaagttgca tacttaaaag gcctagcaga aggactagaa
     3961 ataagtgctg aaagtaaaga agggaaaatg atacataaaa tagttgatgc attagaagta
     4021 tttgcagatg ctattgtaac attggatgaa gaacaagaag aacttcaaga ttttgttgaa
     4081 tctatagatg aagatttagc agatttagaa gaagatattg ctgacatgga agaagatctt
     4141 tatgaagaag atgacgatga ggatgatgag gacttcagct atatagaaat ggaatgccca
     4201 aattgtggag agttagtaga aatagacgaa gatttgttat atgatgatga agtagatgtt
     4261 gtttgtcctg attgtaaggc tgtgatatta tcatcagaag atgattgtga tgatgattgt
     4321 acatgtggtg gttgtccaag ttgtgatgat agagagtaaa aattcataag aattaaaact
     4381 gcctatgatg cctatgagat attttgcttc ataggtagtt ttatttttat gaggaagggt
     4441 attatgtatt ttggttttgc aatacttgga caaaaatagt acactagaat ttatagaata
     4501 ggattttgga gggatgtaaa tatgaaaata agtaaaaaat tactagataa tatacaaaga
     4561 tttgaaaatg ttataaatta taaatttaaa aataaagaat atatactaga agctcttact
     4621 catagttcat attctaatga aaataaaaaa tataatttta acgaaaggtt ggaattttta
     4681 ggggattcgg ttttaggcat agtgataagt gattatttat ttaatgaaga agctaatctg
     4741 cccgaagggg aactaactaa acttagagca aatatagttt gtgaagattc gttaagtgaa
     4801 gtagctaatg atataaattt aggtattcat atgttattag gtagaggaga agaagcaaca
     4861 ggaggaagac ataggacttc aatattagca gacgcatttg aagctgtaat agctgctata
     4921 tatcttgatg gaggttttga aagtgcaaga cagtttatac ttcatcatat ggaaaatata
     4981 atttatgatt caagaaaagg aaatatattt agagactaca aaacgcatct tcaagaagtg
     5041 ttacaaggga atggagaaaa taatatctgg tacaggctta tagaagaaaa aggaccagac
     5101 cataataaaa gatttgtaat ggaagttgga attaatgacg atgtattggg tataggagaa
     5161 ggaaagagta aaaaagaagc tgaacagtta gctgctaaaa tagctttaaa gaaaaaatta
     5221 tgggaaaaat aaatataata tgtaaaaata taaaaagaaa tatttatatt agaaggatgt
     5281 tgtagatgaa aaagagaatt atacctatat ttgttcctca caaaggatgt cctcatgatt
     5341 gcatattttg taatcaaaaa aaaataacag gtgtgagtac tgatgttact agtgaagatg
     5401 caagaaatat aattgaggaa tgtttagaga ctatagataa agatgcagat gtagaaatag
     5461 cattttttgg tggtagcttt actgctatag atattgatat acaaaaaagt ttattatcag
     5521 ttgctaaaga atatgtagaa aaaggtcttg taaaagatat tagaatgtct acaagaccag
     5581 attgtattga tgaagatata ttaagtatgc taaaagaata taagacaagt ataattgaac
     5641 taggagtaca atccttagat gaaaaagtat tattagatag tataagaggt catcaatctg
     5701 aaattgtata caagagttca aagatgataa aaaatagtgg aatcaaactt ggattacaga
     5761 tgatgattgg tcttccagct gacacagaag aaaagtgtat atttacagca aaaaaatttg
     5821 tagagctaaa accagattgt gttagagtct atccaacttt ggtagtaaaa gatacaggtc
     5881 ttgaaaagtt aatggaacaa aataaatata atccatttac tcttgaagag agtgtaaata
     5941 tagtaaaaaa agttcttgta ctgttttatg ttaataatat caatgtaata agagttgggc
     6001 ttcaagctac tgatgatata caaattggaa aagctgtttt agcaggacca tatcatcctg
     6061 catttagaga gttggtagaa gcagatatga taaaagatta tttagaattt gttattttgc
     6121 aaaataaaaa tataaagcaa atgttggtca aatctaataa aaagaatatt tctaaaatca
     6181 taggaaataa aaaaacaaat gttaaatata tggaagaaaa atttggtgtg cttttaaaaa
     6241 cacaagaatc cgatttagat ataaatcaat tagaaattgt gttagatggg aaaagtttaa
     6301 ttagtgctaa tatgagagac attcatagaa aattgtatga catttatgac ctttagtttt
     6361 aataatgaag ggagagaaaa gatttgtatt taaaaagact agagttaaag ggatttaaat
     6421 cctttccagt aaaaacagat attattttta aagaaggaat aacagccata gtaggaccaa
     6481 atgggagtgg aaaaagtaat atatctgatg ctgtaagatg ggtattagga gaacagagta
     6541 ttaagagtct tagaggtgat aaactcgaag atgtaatatt tgcaggtaca gatacaaaaa
     6601 aacctatgaa ttattgtgaa gtggctctta ctattgataa ttctgaaaat caattggaat
     6661 tagattttac agaagtaact ataagaagaa gggcttatag aaatggtgaa agtgaatttt
     6721 ttctaaataa taaaagttgt agattaaaag atataaaaga agtcttttta gatactggta
     6781 ttggaaaaga tggttattca atcatagaac aaggaaaagt agatgaaata ttaagtaata
     6841 atcctttaag cagaagaaaa gtttttgatg aagcttgtgg catatcaaaa tatagatata
     6901 aaaaacagga agcagaaagg aatttaagta atacaaagga aaatttagaa agaatagatg
     6961 atgtatatat agaaattgaa aatcaattaa agcctctttt taatcaacag acaaaggcaa
     7021 aaaagtactt ggaaataagt gaaaaattaa agatacttga agtaaatagc tttataagag
     7081 aaattgaagg aatagaaaaa gaattaagtg aggttaatga acatcgtaag gttattgaaa
     7141 aggagctcaa tgaaaaagaa gaacaaaaaa atgttgtaga gaaaaagcaa gaagatatta
     7201 ataaagaggt agaggtatta caagatgtaa ttgaaaaatc tgtagattat ataaattcaa
     7261 ttaaaggagt tatatcaaaa aaagagtccc aaataaattt aattaaggaa agaataagaa
     7321 attttacgaa tgaaattagt agaaaaaatt tagaaataaa tgatataaaa gagaagctaa
     7381 atgaaaataa gcaatacata aaagaattag aaagtaataa attatcaggt agtgaagaac
     7441 tatctacctt gcaggaaaat ataaaagtat tggaggcaag taaagataaa caaaaaatta
     7501 agttagaatc cctaaata
//
LOCUS       DAEPOZ010000183         7507 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.183, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000183 DAEPOZ010000000
VERSION     DAEPOZ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7507
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.183"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            113..421
                     /locus_tag="KR440_002883"
     CDS             113..421
                     /locus_tag="KR440_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699895.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(583..2373)
                     /locus_tag="KR440_002884"
     CDS             complement(583..2373)
                     /locus_tag="KR440_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0699896.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(2373..4100)
                     /locus_tag="KR440_002885"
     CDS             complement(2373..4100)
                     /locus_tag="KR440_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0699897.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSARVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            complement(4345..4909)
                     /locus_tag="KR440_002886"
                     /pseudo
     CDS             complement(4345..4909)
                     /locus_tag="KR440_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
     gene            5198..6310
                     /locus_tag="KR440_002887"
     CDS             5198..6310
                     /locus_tag="KR440_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBH0699898.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     AEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            complement(6493..7320)
                     /locus_tag="KR440_002888"
     CDS             complement(6493..7320)
                     /locus_tag="KR440_002888"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBH0699899.1"
                     /translation="MSRKYSISSMCKLIGVSRSGYYKWLSYSKKSSDRGIKDRIIKDY
                     IIEIHKKYRGTYGRKRICTYLNKILDSPINHKKVYRLMKELGIKSIIRKKVYRRKFKS
                     YEVYDNILNREFRANQPLEKICMDITYIPIGKKFLYMNVAKDLFNGEIVAYEISTKMD
                     TKLVNKTVNQLINMNLAKDCILHTDQGSQYTSRSYSKRLKDNGIIQSMSRRGNCWDNA
                     PIESFFSHFKSELIYLIDTTDPKEMISLINDYIYFYNNERIQLKNGMSPIEYRTHSA"
BASE COUNT         2550 a         1194 c          932 g         2831 t
ORIGIN      
        1 ttataaatta tagttttaga tttataattt ataaaatatt tatgttcttt gtaaaatttt
       61 ttagaagctt ctaatgttat gctataaaac tatcttgaga agggctgatt gaatgtttaa
      121 taaaatatat gaaaagaaag aatttatagt ttttcaagtt aaaaaaggtt atgtagttta
      181 taatactaga aagagttttg aagaaggtca tacacattta aagcattttg aagcagcaaa
      241 gacagctatt gatttagcaa taaataaaaa gattcctaga agcaaagatg gatattatct
      301 gactagttta attcgcataa gtgatgatgg ttattacatt gataagttaa gcgaattgtt
      361 atatgttaga gaacaaaaag gtaaaaaaga aaagtactgt aattctggtt atcagatgta
      421 acaagactag ttgtgaatta taagtaccaa tcataaatac ctttagtctt aaaatcaaag
      481 agtatcagta aatctataaa aaaacaactt actgatactc tttttaatta tatgattaat
      541 atttgaattt gatacaaaat tttatacaaa agtttatgtt atctattctg attctttgtc
      601 tgcaaattgg ctattataaa gcttctcata aaatccaccc ttttccatta attcatcatg
      661 attcccctgc tctataatac ttccattatt cataactaaa atcaagtcag cattgcgaat
      721 tgtagaaagt ctatgtgcta taacaaaact agttctacca ttcataagat ttctcattgc
      781 tttttgaagc ataagttcca gcctagtatc aacagaacta gtagcttcat caagtatcaa
      841 tattgaaggg tctgatataa aagctctagc tatggttaaa agctgttttt cccctagaga
      901 tatattagat gcttcttcat ttaagaacat attgtatcca tctggaagag tggttataaa
      961 gtgatgaaca tttgctactt ttgctgcttg tataatttct tctttagtag ctccaaatct
     1021 accatattct atattttcaa aaatggttcc attaaataac catgtatctt gaagcaccat
     1081 accaaacatt gaacgcaaat ctttacgttt catatctctt atatctactc catcaatttt
     1141 aatagcacct ttctttacat cataaaacct catcaataag tttattaatg ttgttttacc
     1201 tgctccagta ggtccaacaa tagctaccat ctgaccactt tttacttctg catttaaatc
     1261 ttctattaaa gttgaatttt ctccatatcc aaaattaaca tgctcaaatg taacatttcc
     1321 ttttacattt tctatcttaa ctggattttc aacatcaggt atttcctctt cttcgtctaa
     1381 aatttcaaat acacgttcta tagctgcaaa agaagattgt attactgtag ataactgagt
     1441 catttgtgaa agtggctgat ttatctgcca tatataacgc acaaatgctt gtaaatttcc
     1501 tactgttatc cctccactta ctgcatatat agaaccgaca actgccacag ctgcaattcc
     1561 aaagtaagtt actaatgaaa ccataggatt catcatactt gatataaatt gtgccttaaa
     1621 tccattttca caaagttctt gatttacttt tttaaattct tttatagaat cttcttcttt
     1681 accatatagt tttatttcat taaatccagt atacatttct tgtacttttc catttaattt
     1741 tccaagagca ttttgttgca atgaaaatct actttgagat ttttttacta caaatttaga
     1801 tacaaaataa ctaattggaa ttataaaaag agctataaga gccatatata tattaattgt
     1861 aaacatcatt gaaagtgcca aaattatgac taagattgag tttacaattt gcataaaact
     1921 ctgttgcaat gcatttgata ttgtatctac atcatttgat atacgactta atacatctcc
     1981 aaaactatta ctatcaaaat aactaatagg caatcttctt attttatttt cgacttcatt
     2041 tcttaaatct ctcattgtgt tttgaatagc attcgtaagt agaaaacttg aagtatacgt
     2101 aaaaattgca ttagcaaaat atatacatgc taaaatcacc aatatttttg ttacataatc
     2161 aaaatttacg gaagcccctg gtactccttt tgcgatacta attacatctt tagtcaattg
     2221 tgttattatc aatccttcag ttttaggtgc aagtgcattc atagtagctg ctgaaattat
     2281 acatataata gctataataa aggaaaattt atatttcttt acaaatggaa gaagtctacc
     2341 aattgttttc ttaaaacctt tagacttttt cattattcta attcctcctt ttttaactga
     2401 gatgttgcaa tttcatagta tatttcacag ttttttaata attctttatg agttcccatt
     2461 ccgacaactt gtccttcatt gagtactata atcttatcag catcaataat agaactaatt
     2521 ctctgggcaa caatcaatac aattgcatct tttgtttcct tcttcaattt agcacgaagt
     2581 attgcatccg ttttaaagtc aagtgcagaa aaactatcat caaaaatgta tatttcaggt
     2641 cttcttataa tagcccttgc aatagaaagt ctttgttttt gacctcctga tacatttgct
     2701 ccaccttcac taatcaattc atcaaacttt ctaggttttt ttgatataaa ctcatatgct
     2761 tgtgcaactc ttgcggaata ttctaattct gactctcctg ccttatgttt cccaaatcta
     2821 atattatttg ctatgctacc agtaaaaagc agtgattttt gaggtatgaa tcctatcttt
     2881 tttcttaaag cttttaaatc atattctctt atatctactc catttatctt aatgctaccc
     2941 tctgttacat cgtaaaatct aggtatcaag ttaattagag tacttttccc actaccagta
     3001 cttcctataa aagcgactgt ttcacctgtt tttgcagtaa atgaaatatc cttaagtaca
     3061 gaagcttctc catctggata ggtaaaagtt acattatcaa attctatgat acccttattt
     3121 tcagtatcct taattccatt ttcaggattt tttatcaatg gttcttcatt taaaagttct
     3181 tttattctat ttgcagatac ctctgctctt ggatacataa taaataccat tgaaaaaagc
     3241 ataattgaaa acatggcatg gaaaagatat tccaaaaatg caaccaactg tccaacttga
     3301 agctctccaa tatttatctt ctcacttgaa atccaaaata cagccaaaac agctatattt
     3361 aatagtaaaa agaatgctgg ttgagtaatt gacataagct taaatagttt cttagaaaca
     3421 ttagcataat atgtatttgt tttttcaaat cgctctgttt cataatcatc atttccaaaa
     3481 gctctaatta ctcttattcc agttaaattt tctctcgata taaggtttaa tttatctagt
     3541 cctttttgtt gttttgaaga tattgcctga gatacttttg ctattattgc tactccaata
     3601 attataaaag gtactgatat agccaatact atagaaagtt ccacacttgt tctaattgtc
     3661 attattatac ttatgatgaa cataactgga gttaataagg cagtccttag tagtatatta
     3721 acaaattgtt gaatttgaaa tgcatcattc gttgttcttg taatcattga agaaattcca
     3781 aatttattgt attcagtatg tgaaaattct tgtgatttct caaatatgtc atttcttata
     3841 tctctggtta cacttgttga taccttagca gaacaataac ccaacaatat actacctaca
     3901 acccctataa tagaaataat aacaatcact attcccattt gttttatgta agctatattc
     3961 tgatttgcaa taccattgtc aattatttta gctattatag ttggaatccc caactcgact
     4021 aatgcaaaac taaatatagc cagtatgttt aatactatta ataacttata attctttaaa
     4081 taacttaaaa ttaatttcat atttttctcc tttcttaatt tttgagatag tgtaatcatg
     4141 taataatact aaatttataa atgcttaatt ttataaaaaa aggagaaaag agttaacgca
     4201 ctctcttctc cagcatattc tttttaatac accctccgac aagtcgctta tatgttaaca
     4261 tatttgacaa gtaatgtcaa ttcatatagc taaaagtctt ttaaatgctt catctcataa
     4321 cattgtaaca aattggcatg taatttattt ttgtttttta ctgtattcca attttacaag
     4381 ttctccaata ttactaactt tcttaaaatt caaattagtc ttatctatac cagacttaaa
     4441 tagtgaaatc cctctcccca atattattgg caatatatat atataataat catcaattaa
     4501 gttatctttc ataaataatt ttactatatc gcctccacca actatccaaa tatctttttt
     4561 atccttcttt tttccaattt gctcaattaa ttttttaggt tctaaattag tatactttac
     4621 attctccagt tgtcccatta attgtatttg agtatatata gcattcctta cccttatagt
     4681 accattcatt tgggcttaat tcatttgcaa tctgtacata tgtggaattt cccataatga
     4741 tagtatcaat tgtatcgtaa aaagtattat aatcatcttc ctgctcattc ttaaattctg
     4801 aagatatctc gtctaaaaaa cttacactgc cattctcttc agctatataa ccatctaaac
     4861 ttgtggcaat ataaagtact acttttctca tgtttactac ctccaacatt tatagtgtga
     4921 ttattctatg taaacatcac ttttttactc attcaataat accctattaa taaatcctca
     4981 aactaattta aataaacttt gcataaaact aaaaatatat tatataaata atttaaaatc
     5041 acactaactt attgaaaatt ctaattatta caaataaata ttgctctctg ttaacgctag
     5101 ttttataata taattataag ctattaatat tataaagccg atattactaa tataataatt
     5161 atagttaatt tacttttgtt gaaaggtggt aaataacatg aaaatttcag aagtcgctac
     5221 tccaaatttt ttgctagatt tagaccagtt agaaaagaat atagataaaa ttcaacaaat
     5281 ttgtacaaat aataataaac aactttggcc tatgcttaag acacataaaa gtacttacat
     5341 agcgagactt caaaaaaatg caggtgctag tggttttcta gttggaacac ttgatgaagg
     5401 tgaagctctg ataaaagcag gttttgaaga tattatgttg gcttatcctt acatgggaga
     5461 agtaaatata aatagaattg tgactatggc atcaaaaata aatttaatct gtgctacaga
     5521 taatatagaa tgtgctaagg cttatagtga agctttttct aaatcaaaaa tagagtgcaa
     5581 gctacttatt attgtagatt gtggcttaca ccgttttggg gttgaaccag aaaaagtggt
     5641 tgaacttgct actgaaattt ctaagctaga aaacatcaag attgttggta taagttcgca
     5701 tccaggtcaa gtgtacggtt gtagttctcc agaaggtgtt ccagatgttt gtgttcaaga
     5761 agattcttct atggagaaag catacaaagc tctaactgat aatggattta atatagaaat
     5821 tgtcgcttct ggtagtactc caacagttga agctgaagct gcttctaaaa ctataactgc
     5881 tgtaagacct ggaaactatg tatactatga tgctatacag ataggtttag gttgtgctac
     5941 tgaagatatg tgtgctctta ctgttgtgtg tactgtaatc tcaaaaaata gcagtggata
     6001 ttatttaatt gattgtggaa gtaagtgcct tggacttgat aaaggtgcac atggaaatag
     6061 tagtattgtt ggttatggta gggtatgtgg acatccagaa cttactattg attcactttc
     6121 agaagaagtt ggaaaaatta aagtaaatgg tgaaactgat ataaaaattg gtgatagaat
     6181 aagaattata cctaatcatt cttgttccac tgcaaacttg acgagtaatt tattaggttt
     6241 tagaggagat acaatagaaa aagtcataga agtagatatt cgtgaaaatt caaaacaaat
     6301 catcctttaa aaatatattt attttgatat taacatcatg aaagtatatt tttcttaaaa
     6361 ttattgttaa taactaatgt taatgttgga taaataaaaa acataaattc tagaaatata
     6421 aatttaaaaa aagaggctgt ctcaaaattg gatatgtccc tgtcaagtag acagacttaa
     6481 aaaaggctat ctttatgcgg aatgagttcg atattcaatc ggactcattc cattttttaa
     6541 ttgtattctt tcattattat aaaaataaat ataatcattt attagactaa tcatttcctt
     6601 tggatctgta gtatcaatta aatatattaa ttcagattta aaatgactga aaaaactttc
     6661 tataggagca ttatcccagc agttgcccct tcgggacata gattgtataa taccattatc
     6721 tttaagcctt ttagagtaag atctacttgt atattgtgaa ccttgatctg tatgtaaaat
     6781 acaatcttta gctaaattca tatttattag ttgatttact gttttattaa ctaatttagt
     6841 atccattttt gtacttatct catatgccac tatttcacca ttaaataaat ctttggctac
     6901 attcatgtat aagaattttt taccaatagg tatgtatgtt atatccatac aaattttttc
     6961 taatggttga ttagctctaa attcacgatt taatatatta tcatatacct catatgattt
     7021 aaacttacgt ctatatactt tctttctaat aatagattta attcctagtt ctttcatcaa
     7081 cctatatact ttcttatgat taatagggct atcaagtatt ttatttaaat aagtacaaat
     7141 tctttttcta ccgtaagttc ctctatattt tttatgtatt tcaattatat aatccttaat
     7201 aattctatct ttgatacccc tatcggaaga ttttttagaa taacttagcc atttatagta
     7261 accgcttctt gacacaccta tcaacttaca catactagaa atactatact tcctagacat
     7321 aagtttaatt gttttaaatt tatcgcttac tttgacttgt ccaatagggt acttaacttt
     7381 tttaaaaatt cattctccat gcgtaagtac ttcaattctt cttcaacaga tttaaatttt
     7441 ctattatcat tttctagact aatattttta aatctgcctc tagtttcctc attaaatgca
     7501 gttttac
//
LOCUS       DAEPOZ010000184         7502 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.184, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000184 DAEPOZ010000000
VERSION     DAEPOZ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7502
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.184"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            167..2479
                     /locus_tag="KR440_002889"
     CDS             167..2479
                     /locus_tag="KR440_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBH0699900.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKENNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDTTSNKVNYISDELD
                     TTSNKVNYVSDELDTTSNKVNYVSDELGMALHKVNCVSDELDMTSNKVNYVLDELDIT
                     SNKTDYISNELYNLSNKSIKDNIPIKMAVITKSNMLNQLPDLVELERVFSKIKFKVCF
                     DMFMTDTATLCDMFIPCTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFFME
                     LAKKMGLNDYPFVEKRTYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNLVAWEDKKFET
                     PSGKYELYSESIKNLGISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMDKK
                     SIAQAYINQRMAKKVGIEDKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKKHG
                     NPNSLTDTGISDFGGQVTYNESMVEIIRQN"
     gene            2567..2902
                     /gene="spoIIAA"
                     /locus_tag="KR440_002890"
     CDS             2567..2902
                     /gene="spoIIAA"
                     /locus_tag="KR440_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HBH0699901.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            2916..3344
                     /locus_tag="KR440_002891"
     CDS             2916..3344
                     /locus_tag="KR440_002891"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HBH0699902.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            3367..4131
                     /gene="sigF"
                     /locus_tag="KR440_002892"
     CDS             3367..4131
                     /gene="sigF"
                     /locus_tag="KR440_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HBH0699903.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAVKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            4411..4848
                     /gene="spoVAC"
                     /locus_tag="KR440_002893"
     CDS             4411..4848
                     /gene="spoVAC"
                     /locus_tag="KR440_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HBH0699904.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            5002..6015
                     /gene="spoVAD"
                     /locus_tag="KR440_002894"
     CDS             5002..6015
                     /gene="spoVAD"
                     /locus_tag="KR440_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HBH0699905.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            6029..6391
                     /gene="spoVAE"
                     /locus_tag="KR440_002895"
     CDS             6029..6391
                     /gene="spoVAE"
                     /locus_tag="KR440_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HBH0699906.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            6574..>7502
                     /locus_tag="KR440_002896"
     CDS             6574..>7502
                     /locus_tag="KR440_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBH0699907.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFVGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDRSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLV"
BASE COUNT         2996 a          770 c         1406 g         2330 t
ORIGIN      
        1 ttagtatgca aaattctgtg aagcaggaac actgtgactt tagtcatggg aggttcagaa
       61 aatatagcta tgttttatat actgtagttt tgaacataga aagtttaata aattatagta
      121 tatattaatt tttaataagg taagaaagta gaaagaagga tattttatgg atataataaa
      181 aaaattaagt catggatgta cattagattg tcatgattgt tgtaaattca atgtttatac
      241 taaagaaaat aatgtagtaa aaattgaagg agataaaaat catccttata caaaaggttt
      301 tatatgcaaa aaagggatgg ctcatttaga tagattaaat cataaagata gaataaagac
      361 tcctatgctt aaagttgatg gagtatggaa agagatttct tttgataagg ctatagaaat
      421 aatggcagaa aagcttacat actataaaga aaagtataca tcaaagtctg tcatgcatta
      481 tgaccaatat ggaagtgggt ctgtgctaaa atacatagga gatatatttt ttaattttta
      541 tggaggtgtt agcagacata agggtggtcc ctgttggagt gcagggatgc atgctcaaaa
      601 atatgatttt ggagtagcta agagtcatgc tatagaagat atgttaaata gtaaaagtat
      661 atttgtgtgg ggaaaaaatc cagcatatac tacaattcat actatgcaaa taattaaaaa
      721 agctaaagaa aaagggataa agatagtagt gattgacccc atatatacaa aaacagctca
      781 aatagcagat aagtatgtac aggtaaatcc tggaacagat ggagctttag ctatagctat
      841 ggcaaaaatt atagtagaag ataaattata tgatgaagaa tatataaact cgtatgttat
      901 tggttttgaa gaatataaaa aatatttatc ttcattggaa ttaagttttt taattgatga
      961 atgtggtgtt aaagagaatg atataagaga gttagtggat ttatatacta acaaatattc
     1021 aagtataaat gttggatatg ggcttcaaaa atataaaaat ggtggaaaca caataagagc
     1081 gatagatgct ttaggtgcta taactggtca aataggattt agtggtggag gggtaaacta
     1141 tgctaataaa gtttatccaa gtgttataaa ttctgaccct tacaatagcc aatcatatgg
     1201 tgaagatagg gagttttatg taagtaatat cagtaaattt atagaagaat ctttaaaaaa
     1261 tacatcaaat aaagtaaatt atgcatcaga tgaacttgat acaacatcaa ataaagtaaa
     1321 ttacatatca gatgaacttg atacaacatc aaataaagta aattatgtat cagatgaact
     1381 tgatacaaca tcaaataaag taaactatgt atcagatgaa cttggtatgg cattacataa
     1441 agtaaattgt gtatcagatg aacttgatat gacatcaaat aaagtaaatt atgtattaga
     1501 tgaacttgat ataacatcaa ataaaacaga ttatatatca aatgaattgt ataatttatc
     1561 taataagtca atcaaagata atattcctat aaagatggct gtgataacta aaagtaatat
     1621 gctgaatcaa ctgccagatt tagtagaatt ggagagggtg ttttcaaaaa ttaaatttaa
     1681 agtatgtttt gatatgttta tgactgatac agctacttta tgtgacatgt ttataccgtg
     1741 tacaaataca ctagagagtg aagatataat ttatagctca atgaccaatc catatataac
     1801 ctataatgaa agagctgtaa agccagcaca taaattgatg gatgaatatt atttctttat
     1861 ggagctagct aaaaaaatgg gattaaatga ttatcctttt gtagaaaaga gaacttattt
     1921 agaaaaagtg attgaaccac ttaagcgttt tgacaaaaat ctggatatag aaaaacttaa
     1981 aaacaattac tttactatac ataaccttgt tgcttgggaa gataagaagt ttgagactcc
     2041 atctggaaaa tacgagcttt attcagaaag tataaaaaac ttgggaataa gtccaacacc
     2101 tgtatatata agcaataaat ataaagaaat agaagataaa aatatttctt ttagattatt
     2161 aactaatcat catgcagata ctttatttag tcagcatttt atggacaaaa aatctatagc
     2221 tcaagcatat ataaatcaga gaatggctaa aaaagtaggt attgaggaca aagatattgt
     2281 gatattgagg tcaaaaaagg caaaaataaa tgtacaaata aatatagatg atggagtagg
     2341 taattatata gtaaaaatgt atgttggttg gtggaagaaa catggaaatc ctaatagttt
     2401 aacagacact ggtatatcag attttggagg tcaggtaaca tataatgaaa gtatggtaga
     2461 aataatacga caaaactagt taaatttcat tgaaggaata aaaatataat tatagaattg
     2521 attaaaagtt actattttta caaaactatt gaaggaggga tttttgatgg taaattattc
     2581 cttagaacat aagaatctat acatagaatt tatgtgctca gaactagacc accatgtagc
     2641 caatgaaata agggaagaaa tagataactt attaagtgta aatcaagtaa aaaatgttgt
     2701 atttaatttt gaaaatatta attttatgga ttcttcaggt ataggcgtca tcataggtag
     2761 atataaaaag atttctaatg aagggggacg agtttcagta attaatataa gttcacgagt
     2821 taagaagatt tttgatttat ctgggttaaa taaaattatt ggcatttatg atacgtatga
     2881 agaagctcta agttctttgt agggaggagt atgtgatgaa taatattatg gaagttaaat
     2941 tttcggcaat atctgaaaat gaaagtttag caagagttat agtagcatcc tttgcagcta
     3001 agttagaccc aacagtagat gagttagtag atataaaaac tgctgtctct gaggctgtta
     3061 cgaatgctat aatacatgga tatgaagaag acagttctaa gtttgttttc ttaagatgtg
     3121 agatagaagg aaatactata aagattatag ttgaagatga agggtatgga atagaaaatg
     3181 tggagaaggc aatggaacca ctttatacat caaaaccaga acttgataga tcaggaatgg
     3241 gattcacagt aatgaaaagc tttatggacg atgtagaggt aagttctgta aaagataatg
     3301 ggactagaat tgaaatgact aaaaaaataa atgtgcctaa ataaagtcca aaagtgaggt
     3361 tttattatgg aagtaactgt tgccagagaa gaaaaaaaac ctcttcttag tcatgaagag
     3421 actctagaac ttattgaaaa agttcaaaat ggagatgaag aggcaaaaga aatattaata
     3481 tcaagcaatt taggtctagt taggagcgtt gtatctagat ttttaaatat aggatatgat
     3541 agagaagatt tatttcagtt aggttctata ggcttaataa aatcaatata taaatttgac
     3601 ccaaaattta atgtaaaatt ctctacttat gcagtaccaa tgattttagg tgaaataaag
     3661 agatacttaa gagatgatgg tatgataaaa gtatcaaggt cgttaaagca aatagctgta
     3721 aaggcaaaga tggaaagtga agctttaact aaaaaattag gtagagagcc aagtatagaa
     3781 gaattagcta aagcaataga tgtagaaaaa gaagatttag taatggctat ggaagctaat
     3841 ttcaatgttg agtatttgca aggagttatt catgaagagg aaggttctcc aatatgcctt
     3901 attgataaaa taagcatgaa aggagaaagt gaagaagaaa aagtagtgga taacatattg
     3961 ttaaaagaca tacttggaag actggataag agagagcgtc aaataatagt tctcagatac
     4021 tttgaagaca tgactcaaag tgaaatagga gagatgctca atatatcaca agttcaagta
     4081 tctaggatag agaagaaagt attgtctaag ttaaaagaat atatatcgtg ataatactta
     4141 tgatatgtag aataacaaaa tataataaat atattactaa aataaaatgt aataactttt
     4201 tatgttaagt ttttaaatta ttaaaagata ctaaaataat aaaaagcatt aaaaaagtaa
     4261 aaatataaaa acaaggtgtc tttatggaaa aatagatttt ttctttatta ggcatctttt
     4321 ttattttttg accaaacttt agatagtcaa aataaataat aggtttattt ttttaaaatt
     4381 aacaaataat actttagaga ggtgtttagt atggataaaa attataaaaa atatgtagac
     4441 caaataagcc caaaacctac atatttaaaa aactatacac ttgcatttat tgtaggtgga
     4501 attatttgta tgatagggca agcaataaat gatttgtata tgaaagttgg aggtcttgat
     4561 aaactaggtg ctagttctgc aacatcaata acattaatat ttataggggc atttttaact
     4621 ggattaggag tttacgacct aataggaaaa agagctggag ctggttctat aattccaata
     4681 acaggttttg ctaactcaat agtatctcca gcaatggagt ataaaagaga aggctatgta
     4741 ttaggtgtag gtgcaaactt atttaaaatt gcaggaccag ttttagttta tggtataggt
     4801 tcttctattt tatgtggaat tatttattat atttttaaga tgttttagat gatttttatt
     4861 taatttaaat tttattaatt atgaagaagg tataaaatct gaaaatagct actaaagtaa
     4921 ctaattgaaa aataattgtg cagatttaat taatattaaa aatttgaata gtaaaatgca
     4981 aaattaaaaa ggtgtgaaaa tatgaaaaat aaaagaattg gaaaaagaac agtcaagtta
     5041 gaaaataaac caaccataat atcaactgga actatagtgg gtcctaagga aggtgaagga
     5101 ccactaaagg actattttga tatgattatg actgacgatt tatatggtga aaagacttgg
     5161 gaattagctg aaagtaaaat ggtagagact gcatcccaac aagctatcca aaaggcaggt
     5221 aaaaaattgt cagatgtaaa ttatatgcta ggtggcgatt taataaatca aatagtgcca
     5281 gcatcatttg cagcaagaga actggctatt ccatttttag gtatatatgg agcttgttct
     5341 acaatggcag aaggtctttg tataggttct atgcttatag atggtggttt tgctgattta
     5401 gtactgtcag gtacatcaag tcactattgt acagcagaaa gacaatttag attcccactt
     5461 gaacttggaa atcaaaaacc aatgacagct cagtggacag ttacaggagc aggaagtgta
     5521 ttattagctc ctaatggaga cggtccaaaa gtaaagtatg ttactgttgg taaagtaata
     5581 gatgagggta ttgatgatgg taataatatg ggaccagcta tggctccagc agcaatagat
     5641 actatctact cttactttga agatactaat gatgacccaa atagctttga tataattgct
     5701 actggagatt taggtactct tggaaaacag atagcagaag actttctaaa agaaaaaggt
     5761 gtagatatat caaaagttta tactgactgt ggtatagaga tgtttaacct taaagaacaa
     5821 gatgtccact gtggtggaag tggatgtggg tgttcagcta cagtgtttgc aggatatata
     5881 tatgataagt tgagaaaaaa agaatttaac aaagtaatgc ttgtatcaac aggagcacta
     5941 ctttcaccga caagtacact acaaaagcag acaataccat gtgttgcaca tgcagtagta
     6001 atagtaaatg agtaggaagt gatataatat gttaatggac tatgtaagag tatttattgt
     6061 aggtggaatt atatgtttaa tagcccaagt aatgatggat tatttcaaaa tgcaaactcc
     6121 atatgttatg gtaacgtatg taacaactgg tgtagtttta acttttctag gactttatga
     6181 gccattagtt aagtttgcag cagcaggtgc aacagttcct ataataggat ttggatattc
     6241 acttgctaca ggagtaataa aatcaattca aagtgatggt tttttaggta tttttttagg
     6301 aggtacaact gctacagctg gtggaatagc agcagctata ttctttggat atattatgtc
     6361 agtagtattt actccaaaag caaaaccata aatctttatt aaaagaataa taagtttttg
     6421 aaataataac tttttataaa gcaggtggat atcttttaga ttccacctat tttttatata
     6481 aagaagcgtt tacataattg aagttgacaa ttatttacaa catagtgtag tattatatta
     6541 tcaaaataaa atgaagtgaa gtaggggata aagatggtaa ctattaaaga tattgcaagg
     6601 aatctaggta tcagttattc aactgtttct aggtgcttaa acaataatcc taatgtatct
     6661 gaaaaaacaa aaaagaaagt agtagaagag gcaaatagac tagggttcca ttttaatgta
     6721 aatgcaagaa atttagcaaa aaaagaaact aacaggatag gggtaatctt ttcaaataat
     6781 tttaatcatc aagatactag aaagtttttt agtgatataa tggatagttc tataaattct
     6841 attgagacta ataaatacga ttttataata cagcctaata ataacattag tggtgatagt
     6901 aatgtatata aaatggtaaa tggacaaatg gttgatggat tagtaattgt gtctcaatca
     6961 ataaaaaaag aagagtatga ttttttaaaa gacaataatt ttccacatgt atttatatat
     7021 tttaaaccat cttttgtagg agaagtagac aatttctttt gggacgataa cgtgtatgga
     7081 ggttatatag ctaccaagca tttaattgac catggtcata aagacataat aacagtaact
     7141 tcagatgata ggagttcaaa aatgcatgaa gatagaacta agggatattt aaatgctatg
     7201 gaagaggcaa atttaaagac cgaagtcatt aaatctaaaa tggattttga atctcaagtt
     7261 gaatttttag aaaaatatat tgataaaata aaaaaagctt cagctatatt tgtacaacaa
     7321 gatgtacctg cgatttctat aatacaagag ttaaaaacta cttatggtat aaatgtacca
     7381 gaagatattt ccataatagg gtataataat attgaattaa tatcatattt taggtcgcat
     7441 ttatcaacaa tagatgaccc aagagagcaa gttataaaaa atggagtaga tagtttagta
     7501 aa
//
LOCUS       DAEPOZ010000185         7469 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.185, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000185 DAEPOZ010000000
VERSION     DAEPOZ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7469
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.185"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            313..2502
                     /locus_tag="KR440_002897"
     CDS             313..2502
                     /locus_tag="KR440_002897"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HBH0699908.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEEFIDIKTDLEKVTKANISIRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
     gene            2570..3337
                     /locus_tag="KR440_002898"
     CDS             2570..3337
                     /locus_tag="KR440_002898"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBH0699909.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            3395..4411
                     /locus_tag="KR440_002899"
     CDS             3395..4411
                     /locus_tag="KR440_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBH0699910.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVYAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            4477..4725
                     /locus_tag="KR440_002900"
     CDS             4477..4725
                     /locus_tag="KR440_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699911.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELGVLLEEKKELEEE"
     gene            complement(4902..5399)
                     /locus_tag="KR440_002901"
     CDS             complement(4902..5399)
                     /locus_tag="KR440_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBH0699912.1"
                     /translation="MYKIGESVMYPKEGACYISGLVTKDINHHIQKYYELTVIYNSNL
                     KISIPVLNADKIGVRPIMDENEVENFIQSLDKVDCLWVFDRKKRLKLYHDKFHSGDVF
                     EIVKLIKMLMIQDNSKQLCSTDKDFLNKAQRFALSELAAAQCKSYTIVLEEMKNHILN
                     SKNNN"
     gene            complement(5640..6704)
                     /locus_tag="KR440_002902"
     CDS             complement(5640..6704)
                     /locus_tag="KR440_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0699913.1"
                     /translation="MIRLTIANIKRYIKNHALLVNMVLFPVILMFYLNFFTGNSENQS
                     MYFSPVAIVSNSSGKYEHKLIDSSKLKENSFGLNEQDKAMDLLKNNKVSAVFILDKNF
                     SSNIDNLKRPEVKCFKTENGGGSLWAESQIESFITNSLKLKIDRNIDDKLTKTNIIDN
                     SSGKNKNSFLIVFLICYFMYINAAHLASDLFSLKKSNTLKRLVSTKHNDIKIIFSIFL
                     GLFFTQAIVYTLTLVCFTSISNFKLTLNTLMIVLANSFVATGFVFWIARVFKNEGSIS
                     LMSTFYSLIGLAISISNMIPSLDKLSFMANLSKLTPFYWTIDAINNNGNILINIIVLI
                     LIGMVFVTAGSLNLRDFARN"
     gene            complement(6707..>7469)
                     /locus_tag="KR440_002903"
     CDS             complement(6707..>7469)
                     /locus_tag="KR440_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0699914.1"
                     /translation="SIEKLKSNDNIAILLQDILDTYHEKLYLNNPQKLSSEDFSKLFN
                     KNSIDTSIIKNNVKQNSYEYFALVSLGFFAIIFIMNSILSNYVSESKGLSKRLYSIPV
                     TRVQFLIYDFIGFWIYSFIFLSLYVLFFRTLGVTFKGNFVILALLCALASYFMTSIST
                     FITTFFSKKYGTVIVYALLFLQTVFGGIFSIVNDVFTKFTSLSPTYLIGTLFSNYETF
                     KTIDSIESLIFTCLIASTVLIALSIVKEKYKWREV"
BASE COUNT         3002 a          777 c         1284 g         2406 t
ORIGIN      
        1 agaaaaaata aagaatttat tttctgaata cttattaatt aatcaaagtt aaaatgaaaa
       61 aaatataaaa aaataaagta tataagagta taatttgact tttatatact tttttatatt
      121 atttttgagt tttttaggaa gatattttac aagttaagaa aactataagg agtgttatat
      181 tgaatataaa atagtataat atatattatg ttagataatt aatatgaaaa tatacaaata
      241 tataaaaaag tttgtttaga gttatagtta atgtgtgtat taatttaata ctaatttatt
      301 ggaggtttaa aaatgaaata cataataaaa agagatggta caaaagaact ttttagtaaa
      361 agtaagattg aagatgctgt ctataaagct agtataaata gtgaaggtgg agtagataga
      421 gagttggcat atgatatatc aagaaaaata gctaatgact ataattctat ggaaaaatgc
      481 ttatccgtag aagaaataca agatatagtt gaagtactac ttatggaatc aaatagaaaa
      541 gatatagcaa aacactacat attatataga gaaagacgct ctgaaataag gactaaagct
      601 tgggagtttg atgaattaca aaaatcaata tggaagaaca agtatagaca taatggtgag
      661 agttttgatg aatggataaa gagaatttca aataatagtt caagaatatc aaaactaata
      721 agacaaaaaa gatttctatt tgctggaaga attttagcaa atagaggatt acagaaagaa
      781 ggaaaaaaag taacatattc taattgttat gtaatagaac caccaaaaga tagcatagaa
      841 gatatatttg atactgcaaa aaaacttgct agaactttta gttatggtgg aggagttgga
      901 attgatattt caaagctaag accaaaagga gcgaaagtta acaatgctgc aaaacatacg
      961 actggagcag tctcttttat ggaactttac agtatgacaa ctggcttaat tggtcaaaga
     1021 gggagaagag gagctcttat gatttctatg gatgtaaatc atccggagat agaagagttt
     1081 atagatataa aaacagattt ggagaaggtt actaaagcaa atatttccat aagggtaaca
     1141 gatgaattta tgcaagctgt aagggaaaaa aagttatata attgtacttt tgaagttgaa
     1201 actactggag aaaaaatagt taagatagta aatgcttatg atttatttaa taaatttgtg
     1261 aaaaataact gggattatgc agaaccaggt atattgtttt gggataaaat aaaaacatat
     1321 aattttatga gtgaagataa gaacttttgc ttttcaggag taaatccttg tgcagaagaa
     1381 ccacttccaa gtggaggaag ttgtctactt ggctcaataa acttgtcaga gtttataata
     1441 caaccatttg gtgaacatgc tacttttgat attaataaat taaaatcttg cgttagagaa
     1501 gctgtagtag ctttaaatga tgtcttagat gaggggttga acttgcatcc attacaagaa
     1561 caaagagata gtgtacataa gtatagacag ataggtttag gtgtaatggg tatagctgat
     1621 atgcttataa aaatgggaat aagatatggt tctgatgaat ctttagaact atgtgattta
     1681 attggtaaga ctatgttaaa tgaagctgtg aagcaatcag cattaattgc taaagaatat
     1741 ggaacattta gtgagtataa atatgaaaat atttctaaat ctaagttttt tatagaaaat
     1801 ctaaatgaag acactaaaga aatggttaaa aagtatgggc ttagaaattc acaactttta
     1861 accatacctc ctacaggttc tatatctaca atgataggta tcagtggtgg aatagaacct
     1921 atatttaatc tttcatatat aagaaaaact gaaagcttgc atgatgagga tgtgtattat
     1981 aaagtatata ctccaattgt taaagagtat atggacaaga aaggtattac agatgaagaa
     2041 tgtttaccag atttttttgt tactgctatg aatataggat atgaagaaag aataaacatg
     2101 caaaaagtat ggcaaaagta tattgatgct tctatatcat ctacaataaa tatacctttt
     2161 gaaactacag ttgaagatgt tttcaatata tatattaagg catgggaggc tggtcttaaa
     2221 ggagtaacaa tatttagaga tggatgtaaa aggagtggaa ttttaataaa tgaaaaaaag
     2281 gaagaaaata ataaagaaga taaaaaagaa aatagtaaaa aagaatatta taaaaataat
     2341 aaaaaagaaa aagataataa agatgaaaaa gccaaagaaa taataaataa aaattttgaa
     2401 gaagatgaaa aatttgtgtg ccctgaatgt ggaagtgaag gaattgcaca tacaggtgga
     2461 tgtagtatat gtttaaaatg tggctatagt ggatgcaatt aaactctaat taaaaaattt
     2521 taatatattt aaaaattaaa tttttaaaaa cgaaagatga gggaatggta tgcaggataa
     2581 aagtgtgagg gagattaaag aaataataga gactttagaa gtagaaaaat atatggaata
     2641 tatagaactg ttaagagttg atgaaagaaa atctgtacaa ggtctagcta taaaactagc
     2701 aaaaaagctg gataatatta gaaaagaaga agaaagattg gaaactataa atatatttga
     2761 gaacgaaggc tatgataaag gttatttgta tattggagga attgatgagg cgggaagagg
     2821 gccacttgct ggacctgttg tagcttctgt tgtagtattt aaaaaagata caaaaataga
     2881 aggtgtaaat gactctaaga aactgagtga ggcaaaaaga gatgaacttt ttgaagtaat
     2941 aaaagaagaa gcactagatt atggaatagg tatagtaaat aatgaagaaa tagatgaatt
     3001 taatatatta aatgctacat atatggcaat gaaaaaggct ataaattgtt taaaaaaagc
     3061 accggattat ttattagttg atgcagcaac aataccaggt attgacatat ctcaaaatcc
     3121 aattgtgaaa ggagattcaa aatctatatc aatagcagca gcaagtatac ttgctaaagt
     3181 tactagggat agtataatgt accaatatga tagagtatac ccagaatatg ggtttaaatc
     3241 tcacaaagga tatggaacta aagaacacta tgaagctatt gaaaaatatg gcataactcc
     3301 tatccacaga aaaagttttt tgaaaaatat actgtaaata taatatgata taatgtatat
     3361 aatataaatt actatagtta atgggggatt aaagatggaa tataatgaat tattaaaaag
     3421 tgctagagaa aatttaaatg gaagttgtaa agtttgtaag gtttgtaatg gggttgcttg
     3481 tgcaggtgaa gtgcctggca tgggaggtaa aggaagtggt tcttctttta tagaaaatag
     3541 caagagttta gaaaaagtaa aagtaaatat gagagtaata catgatgtat caaatcctga
     3601 tacatctata gagatgtttg gaaagaaaat gagtgcacct atatttgcag ctccagtaac
     3661 aggaactact ttaaatatgg gtggaaaaat taatgaaaga gattacatag aaccagtagt
     3721 tgcaggatgt gctaatagtg gtatttatgc aatggttggt gatacggcag tagatgcttt
     3781 tttaatggaa aatttagatg tagtcaaaaa gtataatgga gctggaattg tatttattaa
     3841 accttgggat aatgaaaata ttataaaaaa gataagatta gctgaagaag ctggtgcatt
     3901 tgcagttgga gttgatatag atgcctgtgg tctagtaact ttatcattac atggaaaacc
     3961 agtacttcct aaaaatgtta aacaaataaa ggagttagta aagtctacta aactaccatt
     4021 tatattgaaa ggtataatga ctgttgaaga tgctttaatg gcagtagaag caggtgtgta
     4081 tgcaatagtt gtatcaaatc atggaggaag agtgcttgac tgtacaccag gagcatgtga
     4141 agttttacca aaaattgctg atgctgttaa aggtaaagtt acaatacttg cagatggtgg
     4201 agtgagaact ggtttagacg tactaaaaat gataggttta ggtgcagatg cagtattaat
     4261 aggaagacct tttgtgactg cttcatttgg tggtgcaact gatggtgtag aaacttatgt
     4321 aaataaactt caatcagagc taagtagttc tatgatatta actggatgtc aaactataaa
     4381 agatattgat ggaaaagtta tatacaaata aaattttata caaaaaagag gaaaaatcat
     4441 tttaatgtag aataattgta caaaggggga atttatatga gtagaaaagc ttatgaagaa
     4501 gccttagtag aattagaaaa atttattgat gaaagaaaag aaataattaa aagtgcggag
     4561 gactgtattg ataagtacat agttgacaga acgctacctt ttgattacaa agataaatgt
     4621 gtggaatggc aacaagagtt acttgatatt gcagaggcac aagttttaga agcaaacgaa
     4681 ttaggtgttt tactggaaga gaaaaaagaa cttgaggaag agtaagtttt attactaaat
     4741 ctagggaaaa tcaagaatat gatttacccc tagatttttt tatttaaata aagatgtcta
     4801 tataattaat ctatctttat caatgttaga ttaaaaggtt tgaagaaaaa tagagtaatt
     4861 gtatgaatag ttaaacaatt aaactagtct ataagaccaa tttaattgtt attttttgaa
     4921 tttagtatat ggtttttcat ttcttctaat acaattgtgt aggatttgca ttgtgctgca
     4981 gccaattctg acaaagcaaa cctttgagcc ttatttagaa agtccttatc tgtgctacat
     5041 agttgttttg aattatcttg tatcataagc atcttaatta attttacaat ctcaaaaaca
     5101 tctcctgaat gaaatttatc atggtatagt ttaagtcgtt tttttctatc aaaaacccat
     5161 aaacaatcaa ccttatcaag ggattggata aagttttcaa cttcattttc atccataatt
     5221 ggacgtacac caatcttatc agcatttaaa actggaatcg aaattttaag attactattg
     5281 taaataacag tcaattcata gtatttctgt atatgatgat ttatatcctt tgttacaaga
     5341 ccacttatat aacaggctcc ttcttttgga tacattacac tttcaccgat tttatacata
     5401 tggttatacc tccaaactga attttagata ttgcaatttg ggtacaatgc tttaattatc
     5461 taataattaa tattatatca cgaaatgctt gacaagtcaa aaagattaat atataataaa
     5521 aagtttagaa taatagatat atataattgg ctataaaaat ataagataag aggatttaat
     5581 atattaaaat atctagttag tattgtataa aataacatac taactagatt taataattat
     5641 taatttctag caaaatctct taagttaaga cttccggctg ttacaaatac cattccaata
     5701 agaattaaaa caattatatt tattaatata ttaccattat tattaatagc atctatagtc
     5761 caataaaatg gtgtaagctt tgaaagatta gccataaagg ataatttatc aagtgatggt
     5821 atcatattag atatggatat agctaaacct attaaagaat aaaacgtaga cataagactt
     5881 atagaacctt cgtttttaaa caccctagct atccaaaata caaatccagt agcaacaaaa
     5941 ctatttgcta gtacaatcat caatgtattt agagtcaatt taaaattact tatagatgtg
     6001 aaacatacta aagttaaggt gtatacaatt gcttgagtaa aaaataaccc taaaaatatg
     6061 ctgaaaatta tttttatatc attatgcttt gtagaaacaa gtctttttaa ggtattagat
     6121 ttctttaatg aaaataaatc agaagctaga tgtgctgcat taatgtacat gaaataacat
     6181 atcaagaaaa ctattaaaaa tgaattttta ttcttacctg aactgttatc aattatattt
     6241 gtcttggtca atttatcatc tatatttcta tctattttta attttaatga attagtaata
     6301 aaagattcta tttgtgattc tgcccaaaga ctacctccac cattttcagt tttaaaacat
     6361 ttaacctcag gtcgttttaa gttatctata ttgcttgaaa agtttttatc taaaatgaag
     6421 acagcagaaa ctttattatt ttttaataga tccatagcct tgtcttgttc gtttaatcca
     6481 aatgagtttt cttttaattt agaactatct attaacttat gttcgtattt tccagaagaa
     6541 ttggatacaa tcgctactgg agaaaagtac attgattgat tttctgagtt gccagtaaaa
     6601 aagttaagat aaaacattaa tataacagga aacaaaacca tattaactaa aagagcatga
     6661 ttttttatat atctttttat gtttgcaata gtcaacctaa tcattattaa acctccctcc
     6721 atttatattt ttcttttaca atagaaagtg caatcaatac tgtagatgca attaggcatg
     6781 taaaaattaa actttctatt gaatctatag ttttaaacgt ctcataatta ctgaacaatg
     6841 ttcctattaa ataagtagga gataaacttg taaacttagt aaacacatca tttactatgc
     6901 tgaaaatacc tccaaaaact gtttgcaaga agagtaatgc ataaactata actgtaccat
     6961 atttcttgct aaaaaaggta gttataaaag tagatattga agtcataaaa taagaagcta
     7021 aagcacaaag taaagctaat ataacaaagt tacctttgaa tgttacaccg agtgttctaa
     7081 aaaataaaac gtataatgat aaaaatataa atgagtaaat ccagaaacct ataaaatcat
     7141 aaattaaaaa ttgtactcta gttacaggta tagagtagag tctttttgaa agtccttttg
     7201 attcacttac ataatttgat aaaatgctat tcattataaa aattattgcg aagaatccaa
     7261 gggaaactaa agcaaagtac tcataagagt tttgtttaac attatttttt attatagagg
     7321 tatcaataga attcttatta aataattttg agaaatcttc tgaacttaat ttttgaggat
     7381 tattcagata taatttttca tgataagtat ctaatatatc ttgaagtaat attgcaatat
     7441 tatcattaga tttaagtttt tctatactt
//
LOCUS       DAEPOZ010000186         7408 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.186, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000186 DAEPOZ010000000
VERSION     DAEPOZ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7408
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.186"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(222..1472)
                     /gene="cme"
                     /locus_tag="KR440_002904"
     CDS             complement(222..1472)
                     /gene="cme"
                     /locus_tag="KR440_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HBH0699915.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHNIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLVMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIVAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(1558..2304)
                     /locus_tag="KR440_002905"
     CDS             complement(1558..2304)
                     /locus_tag="KR440_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0699916.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSNPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            complement(2419..3441)
                     /locus_tag="KR440_002906"
     CDS             complement(2419..3441)
                     /locus_tag="KR440_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBH0699917.1"
                     /translation="MNKNNCLDLIFEKVDPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKVVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTCSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWICKKDGVLWYSAMGTGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            complement(3441..5861)
                     /locus_tag="KR440_002907"
     CDS             complement(3441..5861)
                     /locus_tag="KR440_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBH0699918.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLALLYSVEGQIMCTMCDTPVSDNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEIRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKILINQGNLNTFGTNLVNDILSKTRHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSIKKLKDLGNTVI
                     VVEHDKNTIEMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSDSEIGDEDDYAVIETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLERGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            complement(5947..6867)
                     /locus_tag="KR440_002908"
     CDS             complement(5947..6867)
                     /locus_tag="KR440_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH0699919.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHFVDIYLPIEPF"
BASE COUNT         2491 a         1177 c         1053 g         2687 t
ORIGIN      
        1 attattatat gaggtattat tatataaagt taataaagat agtatcaaaa tattatttgc
       61 tataaagaga gggactcaaa ataaagataa acctattttt caatgttgaa atgataaaat
      121 atatttgatt tcatataaaa aattttcttc ataaattaaa aagtccattt taagccccct
      181 catttttaac cagataaatc gagttaaact atttaaatat attactcgga ttcatttgac
      241 ttagatattc cttgcttgta aaattgtttt gaatagggga tagccaaagc aaaaaataaa
      301 agtataatcc ctgctacgat tattaaatta ttaatagaaa ttacatctgc cattggtccg
      361 aaaatcatca taccaagagg aataaaacca gtatatataa taataggtaa gctaagaact
      421 cgacctagca tttgtggtgc tacatgctct tgcaacatag tcatagctga tgcttgaata
      481 attggaattg aaaaattcgt aaaaaacata aatataaaaa attgccaagc ttgtgttaca
      541 agacctagaa taattgaaaa aatagcatag gataatattg ctagaaaaaa ggtcttattt
      601 ctatttttaa aaccaccaaa aactcctata atcaatccac ctaaaaccat aactaaaaat
      661 ccaatcattt caataataga caggtatata taacccttgc caaatgttcg ttcaataaga
      721 agtcctataa gaaatcctga tggaacacaa agaagcatga atgctccata agtgaaaagc
      781 acttttctaa tgaacttatc tgaaaaacag tactttatcc ctgataccat atctgcaaaa
      841 aatgagactt tttcagtttg ttttggtata ttatgtttag gaattttgat gaatatcaat
      901 aatacaatag caataattgc agtaaataca tcaattaaaa gaacttgatt tattttatta
      961 ccaaatccaa gtattgcagc agctacaata ggcactataa attgtactat agactgtaga
     1021 ctactattta taccattaaa acgcattaaa tgttcttctg gtacaagttg aggaatcatt
     1081 gcgtttacag caggtgtttg tacaccactt cctagtgaac gtataacaga tattactgat
     1141 atggctatta attcacttgt tgtgccattt gatgacatca taaatagaat tagaactaat
     1201 gtagatatag caattgttga atctgataca attatgagta cctttcgatt gtagtagtct
     1261 gcccatacac cagcaaaaaa agaaattatc atttgaggta taaaggagca tacagttaaa
     1321 actgttaccc atacacctga tgatgtctga agtgtgacat accatatcat tgccatttgt
     1381 acaagtgatg agccaaataa ggaaatatat tgacttgcta aaaaaagaat gacttttttc
     1441 ttccatgtag aattagtatt tttgttttcc atcctattcc tcctttgatt tgtgtgttga
     1501 atttttcttg ctcttaagga taacacacaa atcaaagtct ggagttagag tggagggcta
     1561 ggaaaattgt tttttcataa tttgaagatg agatttcatg gtttttgcat tatgttcagc
     1621 ttctttcaat gaagttatca gtttgtcaca cactgagata atttcgtcat catcttttgg
     1681 agtgtcaagg acagttttaa tattagtatt aggattagaa gataaagcat ttaacattcg
     1741 taaaatagct gaaagagaat agtttgcaca acgaagtgcg cttataattt taagtcgttt
     1801 tatatcttca tcagtatata cacgataacc attttgtttt ctttttactt ttaacaaacc
     1861 attcatttcc caatttctta aagtatctat tgtaacattt aagtgttctg cagtttcttt
     1921 tctagttaaa aagagtttat ttgtttctgg agtatttcca tttaaaatct gtgcaacaat
     1981 agtaagtgct tcctcggcat tagatttttc tatttctaat tgttgcaagt attgatttac
     2041 aagactaata gccttatcaa agtccctatt cgctgatgtt cttacaatct caagggcatc
     2101 ttttcttaat cccctttgta ccacttcaat tttcaaggct gtacgagcaa atctaaattg
     2161 ttctatatga aaatcattaa agatacgata accattggta ttacgcttag gtttaggtat
     2221 taagtttagt tcttcatata aacgtactgt attaggatgt acaccaatta aatctgctac
     2281 ctctgatgtc gtgtaagtgt ccattagtat tcaactcctt ttagatatat agtattagtc
     2341 ataataacac caaacaaaag tctggtgtta tagtggaggg tttggttatc taagagtaat
     2401 tagtttatct aagagtgatt aatctattat agaagtaagt atatattttt caataaactc
     2461 ccatctatca cgaggtttaa acactatttt agatggtatg gctattacta agtcatattg
     2521 tggaatacaa caaatagcat ttcctccagt tcccattgct gaataccaca aaacaccatc
     2581 ttttttacat atccaccaca gataaccgta ttcattacta ttcattttaa ccgattcttc
     2641 aatccacgat ttagatataa tacgtttatt attccaaata ccatcattta aatacagaaa
     2701 accaaatcgc aacatatctc tagcactcaa tgtaagtccc cagccaccta tagaataacc
     2761 ctgtctatct tttgcccaac ccttaagatt ttttccaaag aaatcttcaa aaccaaaatc
     2821 tttcatatca taatctggta taactttcat accaataggt ttaaataagt actcattagc
     2881 aaattcacga gcagattttc cagtagtttt tgttataata gctgaaagta ggtgcgttcc
     2941 agctgaagaa tatttgaaag taccaatttc gcctttttga ccaagttgat tcaaggtata
     3001 ttttatccaa tctttttgtt tgcaaagtct ttctaaagac tcagtcatat ttttaaaagg
     3061 gtatggtgca gtcattgtaa gtaggtgacg tatagttact gcttttttac atatttcaga
     3121 aggactacaa gtatattcag ggaaaaaatc aacaaccttt tggtcaacat cttttatata
     3181 tcctttatct attgcaatac ctattaaggc tgatataata ctttttgtaa ctgatgcaac
     3241 atgaaaagta tcatcagcat tatatccatt atagtacttt tcaaagacaa cttttccttt
     3301 tcttgcaata attattccat ttatattact atattgtgag attattaaag gttccagttg
     3361 tgagagcttt tctgaactca tgccaacact tgaagggtct actttttcaa atattaaatc
     3421 taagcaattg tttttattca ttaaagatac ctccctgtta tagattttgg attttctttt
     3481 agagtttttg gagaaccttc tgcaattata taacctcctt tgtcaccacc ttctggacca
     3541 agctcaacaa tccagtcaca aacatttaat acatctatat catgttcaac aacaattaca
     3601 gaatttccat tttccaccaa ttcatctaat aattgaatca acttagaagt atcatataga
     3661 cttagaccag tagttggctc atccaataca taaaggatat taccatttcg ttttttacca
     3721 atttcttttg caagtttgat tctttgggat tcaccaccac ttaaagtcgg tgtaggttgg
     3781 cctaatttta tataccccat acctatttgt tctaatacat ttaagtgtga aacaatattt
     3841 ggaatatcct tgaagaaatg tacagattca gaaacactca tatttaaaat atcatgtata
     3901 tttttatttt tatatttaat agataaagat tcatcattaa atctttttcc ctgacattca
     3961 ctgcatatat ggtctatact tagattattt cctaaccaga ccttttcata tccactacca
     4021 ctacatttag gacaagcacc tttggagtta aatgagaaat gaccagctgt aaatcctcgt
     4081 tctaacgatt ctggctgacc tgcaaatagt gtacgtatct tatcccatat accaatataa
     4141 gaagcaggag ttgagttcat atttttgcca ataggagatt gagatatctc tgaatatcca
     4201 gaaatatgtt tagtaccaat cagtctatct gcaatagttt caataactgc ataatcgtct
     4261 tcatcaccta tttcgctatc tgaagaattt atttgattat tgatagtatg gttattaaat
     4321 tggcttctaa gaagaggaag tagggtgtct gatataagtg aactcttgcc actacctgat
     4381 tttccagata ttccaaccat agcacccaaa ggtaatgata cagttacatt tttaagatta
     4441 tttgtatttg cattttgaat agttatacaa ggtattttat cagtatgtgt aatattttta
     4501 ttgcttgttc tattaggcat tggatatttt ccagatagat actgcccagt aagtgatttg
     4561 tcggattgaa gtagaccttc taaatctcct tgatatacta attgaccacc ttcaacacca
     4621 gcttttggac caatatcaat aatatgttct gccatttcaa ttgtgttttt atcgtgctca
     4681 actactatga ccgtattgcc taaatcttta agtttcttta ttgattttaa gatgtctatt
     4741 ttttctgatt catgcaatcc agctgttggt tcatcaagca cataaattaa tgagtccatc
     4801 tctgaatcga gatgtgaatt taaaaatagt ctttgcattt ccccaccact caaagttgac
     4861 atttctcggt ataaggatag atgacctaat cgtgattcta ttaaatgtcg agttttggat
     4921 aaaatatcat ttacaagatt tgtaccaaat gtgtttaaat taccttggtt tattaagatt
     4981 ttaaggaaat catcaatttc taaaatcgtc atttttccaa gttctccaat atgttttccg
     5041 tttaaaagta ctctacgtgc ttcttcacca attttaaaac cattgcaatc tgaacaggca
     5101 gtcatagtat aaactccatt taaatcctca ccccttttgt aacgattgtg aaataccttt
     5161 gataaagagg aacttttttt accattttca taatgaccat agagtacttc atttcttatt
     5221 tcttcaggta gttgtaagaa ttgtatgtct aaataatcgt tgaatttttt agaaaatact
     5281 ctcaaatatc ctggtgtaat ctttgctcta tctaagactt gttttagggt atcatgctta
     5341 tcaaggacta atttttcaat attaatctca aaatatgcac catgtccaga gcacttcata
     5401 cacattccat ctgaagagtt gtatgagaaa taacttggtc ttaatcgttc ttcctcatac
     5461 ccacaattat tgcaaattag attatcgctt acaggagtat cacacatagt acacattatt
     5521 tgaccttcta ctgaatacaa tagtgcaagc atgttgagaa tattggttct tgtgcctact
     5581 gttgaacgag gattgctctg gcgaataata ttttgttgaa cagctatagt gggagctaaa
     5641 ccttgaatat tatcaaactt atcctcacta tcaatacctg caagtatacc taaagattgc
     5701 agatattgtt tacgaccttc ctcaaataca atgtcaaaca taaggctaga cttacctgag
     5761 ccactgacac ctgttgcaac aacaagctta tctttgggga tagaaacatc tatattttta
     5821 agattgtgta tctttgctcc attaattttt atttctttca ttggttcacc tcattttatc
     5881 aattttatca aaccgtaaaa tagggatata ttaatcctta atacaagaat agattaggtg
     5941 aggttattaa aaaggttcta taggtaaata tatatcgaca aagtgtctat tttgagggtg
     6001 attgtgtggg ttgttaagat atacttcaaa agggaaggag gcacgtagct tataattacc
     6061 atttgatagc cattcattat acataaaatc ccacgcatca ctatattcag attttgaaat
     6121 atcaaaatgt cccacaagat attttccaga agggattatc atattgccaa gattgtcatt
     6181 ctctttgaca acaatgttat ctggaatggt catgcaaata ctagtcctta attgatgctc
     6241 ctgtgtaaat tgtggattat catgataaat ggctaatatt tgtgtgtttt caagtccaag
     6301 tagattctgc tcacttgcat atgtgaataa ccgttctaaa agttttggga aagtactggt
     6361 tagattacca tatgaaccag tatatctagt gtaaatagta ttcatattat caagttctag
     6421 aatttcaatt tcacctttaa cctctctagt attcttacat ttaggttttg atatgcttct
     6481 attgtactga gaaattttat atggttcttt gcaattcttg ctataattgt ttctatattt
     6541 tatagggctt attttatagt aatttttaaa tgctctcgag aaaactgctg aatcagtaaa
     6601 gccgaagtag tgtgcaatat cagtaacagt catgtcagtt cgatgaataa gaaaagcagt
     6661 tgcattttct agttttagcc tatttacata ttgtgccaaa gtttcttttt ctattgcttt
     6721 gaagattcta tgaaaatgaa attttgaaaa acctgctata ttagaaagtt catcaagaga
     6781 aagaggttca tgtaaatgtt cttctatata gtcttgcact ttgcaaatac gatttagata
     6841 ttctttttta cttttagcat ttgtcatata tagccctcct tattgtgtaa aatgtgtatt
     6901 aattaattta tagcaatata gtaataaaat tttatgtttt tgcaatttat gataaataag
     6961 atagcgttta attttttata attgataaaa gatatttata attaataaaa gatataagat
     7021 gccttatatt tatttttgca tataagtata gtcttcgtaa taattagtat accatagtga
     7081 tttattttta attgtgtatt tatataaata cacaacttat gatatggacg aattattatg
     7141 caaataagct gtaattaaat aaattgcaat ataaaccagt tgaaacataa gttagggaaa
     7201 tattatcaaa gttattattt agcataagag tatagaggga ataaaataag attaaaaaat
     7261 tacatactaa atttaataaa attattatat taaaaataat attaagttta ttgtttaaat
     7321 aaaaaagtta aatctacact attaacttat tatacttaaa actttaaaat gaaaatatca
     7381 aagaatactt tttaataaaa ataatcat
//
LOCUS       DAEPOZ010000187         7261 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.187, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000187 DAEPOZ010000000
VERSION     DAEPOZ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7261
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.187"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..846)
                     /locus_tag="KR440_002909"
     CDS             complement(<1..846)
                     /locus_tag="KR440_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699920.1"
                     /translation="MNKKDTMHLIFIIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QELMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECTIDVDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGDISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKAYDNIRLEKVDIIVNEGKKD
                     TIYKAQLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMG"
     gene            complement(1061..2740)
                     /gene="recN"
                     /locus_tag="KR440_002910"
     CDS             complement(1061..2740)
                     /gene="recN"
                     /locus_tag="KR440_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBH0699921.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLKSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGSFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            complement(2760..3572)
                     /locus_tag="KR440_002911"
     CDS             complement(2760..3572)
                     /locus_tag="KR440_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBH0699922.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEYTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            complement(3705..5570)
                     /locus_tag="KR440_002912"
     CDS             complement(3705..5570)
                     /locus_tag="KR440_002912"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBH0699923.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANNNED
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            complement(5604..6041)
                     /locus_tag="KR440_002913"
     CDS             complement(5604..6041)
                     /locus_tag="KR440_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HBH0699924.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            complement(6071..6958)
                     /locus_tag="KR440_002914"
     CDS             complement(6071..6958)
                     /locus_tag="KR440_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBH0699925.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            complement(6961..7176)
                     /gene="xseB"
                     /locus_tag="KR440_002915"
     CDS             complement(6961..7176)
                     /gene="xseB"
                     /locus_tag="KR440_002915"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBH0699926.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
BASE COUNT         2207 a         1414 c          689 g         2951 t
ORIGIN      
        1 tcccatagtt atatcttcag ttatatcctt tgtatattct actccattat tgtatgtaag
       61 tttagcttta atataaatta tgtcatcaat atccctttta ggaaaactta cttcataatt
      121 cacttgattt ctatttgaat tattagtttt gccatcatct aagccttcag ctaattgggc
      181 tttgtatata gtgtcttttt ttccttcatt tacaataata tctacttttt ctaacctgat
      241 attatcgtaa gctttatttt ttgacttttc actctcatga ttaaaaagat tactattttc
      301 tactatttca ataaagtatt ctctattatc tcttatccca gaattaaata taggttgttc
      361 aggcatataa tcagatttag tcagtttttg tacatcagaa ctttcagata tatctccact
      421 tgtaactact ttataatcat attcacttcc tgtatttatc tttatttcac atgcatatga
      481 taattcccca tgttttgtca tctcagtttc attccattta tcactatctt ctttatacat
      541 aaaaaatact tttccatttt ttccaagttt acttaattga acatctacat caatagtaca
      601 ttcttttttc tcgatttttt ttacttttaa gtcaatactt tttgttatat aactttttga
      661 catttcttca tttatagtat tactaatatc cgacttagta ctcataagct cttgtgacaa
      721 ataatctaat ttgttactac aactttcaat atttttatta atctcactta ctgctaccac
      781 aatataccat aataaaataa aaattacaat tataaaaatt aagtgcatag tatctttttt
      841 attcatttta tgcctcccct cactactacc ttaataaata atattatact taagcattta
      901 gaaatttcaa agttacaaat ttatacaaag ttcattccat tttcaaacct acttttcttc
      961 aatataggca ctagatattt taaaataatt tcatcataaa aaataggtgt aaagcatttc
     1021 tataaaaatt ctttacacct attaaaatgc ttgtttttca ctaacatttt ttagccaatt
     1081 caatgatttc acttgcatgt tcaatagttt tttctgttat attgttacct gctataagtc
     1141 tggcaatttc attttttcgt tgagattcat ttaacttact aacattggta aacgtcctat
     1201 tatttgaagt atctttctct atacaataat gagtgtctgc atttgcagca atttgaggta
     1261 aatgagttat acatattatc tgttttttct ttgctatatc actcaatttc tcaccaacta
     1321 tctgtgctgc tatcccactt attccagtat ctatctcatc aaacactaat gtatcaatat
     1381 catctatatc agccaatatg gttttaaacg ctaacataaa tctggacatc tcaccacctg
     1441 atgcaacttt atatataggc ttaatatctt ctcctaagtt aaaagatatc ataaattcaa
     1501 tatcatctac tccatctaaa gtaaatgaac ctttttcaaa attaaccttg aacattacat
     1561 ttttcatatt taagcttttt aattcatcaa gaagtatttt ttctaaagta gttgctactt
     1621 ctattcttgc tttagtcaat ttactggctt ttatttttaa atcttcttct atattcatca
     1681 gtttgctttt taactgttca actctttcat ctctatttaa tatttcatct aatctatctt
     1741 taatttttcc ataatactca aatatatcat ctatactgtc tccatatttt cttcttaaat
     1801 tattaatttc gtcgattcta agctcaatct gctctagctc ataaggttca aagtctatat
     1861 tatctttgta atttcttatc tcaccagata tgtcttgtaa ctcatacata attctttcta
     1921 cagtatcact atattcactt aaaacactgt catatttaga tatgtcatta agttccttag
     1981 atgcaagacc tattaggtca ataacattat actcaccatt atgtaacttg ctataactcg
     2041 aatttagatt attatatatc ttttcactat ttctataaac ttctctttgt tttaataaat
     2101 cttcatattc attcttattt agatttgcag cttcaatttc atttatttga aatcttaata
     2161 aatctatttc tcgttgaatt tgcatctcat ctttattttc tgtcaagcaa tttaaagctt
     2221 tttttacttc actatactta tggtacacat ttttataagc aattttaaac tcttccaatt
     2281 cattttctcc aaaaaggtct agaaatttta aatgagtatc tttattgaac aaagcttgat
     2341 tttgatgttg accatgtata tctataagtg tagatgcaat ttctttaaga agagacactt
     2401 taacagtcct tccatttact ctagcagtac tcttaccatc tgagtatatc aacctagtta
     2461 ttaccaataa attatcatct tccatactta tatcattttc ttctaatatt tttttaagat
     2521 atctattgtc tgaaaaaaat acagcctcaa caagtccctt atctgttcct tttcttaaaa
     2581 aagacctatc atatttatca cctaaacaaa gtccaagtgc atctattatt atagattttc
     2641 ctgaaccagt ttcacctgta agtatattta agtttttatc aatattcaac cttagttctt
     2701 caactaaggc acaatttttc atatataatt cgaggatcaa atctaagccc ccttttaacc
     2761 tatttgtcta aattatgaga agcttcaaca acttctacta ttttgttatt gtatgtctca
     2821 ccatcaaatt catatccttc atttccattt ctcaaatgaa ttagatattc tatattacct
     2881 tcaggacctt taataggaga aaaatctaac cctaaaatct caaatccatt ttcgactgag
     2941 aaatcagaaa tcatttttat aacttctata tgtgtagatt tttctctaac tactcctttt
     3001 ttacctactt tttctcttcc tgcttcaaat tgaggtttaa ttaaagcgac aacttcccca
     3061 tcatgacgta ctaactcctt tgcctttgga agtactaact ttaacgatat aaaagacaca
     3121 tcaattgacg caaaatcagc aaactgcttt gtatattcta ttgtaacatt tcttatatta
     3181 gttctttcca tacacacaac cctatcatct tgtctcaatt tccatgcaag ttgaccataa
     3241 ccaacatcta tagaaaatac ttttattgct ccatttttga gcatacaatc tgtaaaacca
     3301 cctgtggatg cccctatatc catacatacc tttccatcaa tagtcaaatc aaagtttttc
     3361 attgcttttt caagctttaa accacctcta ctgacatatg gtattggatt tccttttact
     3421 tctatactac aatcttcttt tacttctgta ccagccttat cacacctttg attatcaaca
     3481 aaaaccaatc cagccattat tgcttttttg gctctttctc tactttcaaa gtacccttgt
     3541 tctactaata ataaatctat tctttttttc atacaattac ctttctgtta aaagctactt
     3601 taatgataac taaagtagct tgaagtaaca cacttgatat ttattctaat tgataaattc
     3661 tatattttat ataaatttta atttacaata tctacattag atttttactc aataactaat
     3721 ttccttattt tatcagcaat actttttggc gataatccta caaaatcata taattcatct
     3781 atattgccat gttttacaaa ttcttctggt attgctatat taagtatatt tacgttatat
     3841 tcattatcta taataaactt gtttattcta cttccaaatc ctccagttac aatattatcc
     3901 tctatagtaa ctacattttt atgatttttt aataatgctt ttaacatacc ttcatcaatt
     3961 ggctttaaga atctagcatt aactattgtc ggatttattc cttctcttga taatatttct
     4021 ttagcctcta aagcatgttt taccatactt ccaatacaaa gtatgactgt atcttgaccg
     4081 tcatctaata cctcatattt acccagtaca atctctccat attctccctt atctaggtaa
     4141 taactacttc ctcttgggta tctaattgct agtggacaat ctaattttaa tgacaaatcc
     4201 atcataagct ccatctctct tgtgtctttt ggagccatta ctacaatatt tggaatagaa
     4261 tttaagtaac ttaaatcaaa cataccatga tgagtttctc catcatttcc aactaatcca
     4321 gctctatcta taagaaaagt aactggcttt tttgttatgc atacatcatg aataacttgg
     4381 tcatatgccc tttgcaaaaa tgatgagtac acagcaaagt aaggtttcat accatttttt
     4441 gcaagacctg ctgcaaatcc tgtggcatgt tgttcagcta taccaacatc ataatatctt
     4501 tttggatatg cactttcaaa caggtttaac cctgtaccag atggcattgc agctgttatt
     4561 gctactatat cttcattatt attagccata tcaacaagtt tttctccaac actaattgac
     4621 atagacttaa cttttgcaga tgtaacacct gtctttatat caaactttga tactccatga
     4681 tatttatcag gttgttcttc tgcaaatctg tacccttttc cttttttcgt aattacatgt
     4741 aaaagaacag gccctttttt gtgtttagct tttctcaata tatctattag ttctttagta
     4801 ttatggccat ctataggtcc ataatatctt atacctaatg aatcaaaaaa tgaacagtcc
     4861 tgaggagaaa agctatacat tagtttatct ttaaatctat gtgcagtctt agataaaatt
     4921 tcacctgttt gtgtaacatt taaaatttta tcaacttcat ctgtcatttt ctcaactgtt
     4981 gagtttctta taatacttga cagatattta gacattcctc caacattttt atctatggac
     5041 atctcattat cattaaggat tactatcata ttggtatcta tatatcctaa ttgatttaaa
     5101 gcttcaagtg ccatacctcc tgttatagaa ccatctccta tcacagatat aacactatag
     5161 ttttcttttt ttatatccct agcgcaggca atacctgtag ctatagaaat agatgtactg
     5221 ctatgtcctg tatcaaaaat atcatgagga ctttcattct cctttggaaa accacttagt
     5281 ccattaaact gtctcaaaga tacaaaacaa tcttttcttc cagtaactat tttatgaaca
     5341 taagattgat gccctacatc ccatacaatc ttatctttag ggctatcaaa tactttatga
     5401 agtgctaatg ttaactcaac cactcctaga ttagaagcta agtgtcctcc tgtctttgaa
     5461 acagatttta ctaagaactt tcttatatcc tttgcaagta aatccatttc ttctatactc
     5521 atatttttta tatcctttgg agaatttact ttatctaaat atttatacat attatcctca
     5581 cctttattct gtaaattttc aatttacatc aatattaaag ctgttactat tccaagtaaa
     5641 gctccaaacc aaacttctag tggtgtatga cctattaatt cttttaattt cttctgctct
     5701 atatgctttc catgctgtat atctgccacc atttgattta atatagtagc ttgtttacca
     5761 acagctcgtc taacacctgc tgcatcatac attataatta aggcaagtac ggtaattata
     5821 gcaaaatcag tagaattaaa ccctttttca atacctacca ctgtagctaa acttgtaaca
     5881 aaagaactat gggaactagg catacctcct gaaataagta ttcttgataa ctcaattctt
     5941 ttttccttac cagtaaatat ttttataaat tgtgctaaga aacaagcaat cagacttatt
     6001 cccaaagccc cattgttaaa aatctccgaa aaaaaatcca tttttccctc ctcgatttta
     6061 gctgtatatt ttagtattct ctatctatta tatattcagc caatcccttt aaaaaatcta
     6121 catcatcaga taatttttct atacttttct ttgcttcatc aatcaaatta tgtgcaattt
     6181 ctttagattt atctaaacct aaaagagatg ggtatgttga cttatgattt tctatatcac
     6241 ttccaacctt tttacctaac ttagcttcat ctcctactat gtctagtata tcatctacta
     6301 tctggaatga taatccgata ttttttgcat atttagttat ttcttccatt tgttcagaat
     6361 ttgctccacc tatagtagct cctgctctca tacagcctac catcattgca gcagttttat
     6421 tcatatgtat ataatctaat ttttcttttt caatttgttt attttcgctt tctacatcta
     6481 ctacttgacc acctatcatt ccatatatac cagcaccttt ggcaatttca tttatagctt
     6541 ttagatactt ttctggattt tctttattga ttgagcctgc aagcattact tcaaaagcat
     6601 agtttaacaa agcatctcct gctaaaatcc ccatagcttc tccatatact ttatgatttg
     6661 ttggtcttcc tcttcttaaa tcatcattgt caagagctgg caaatcatca tgtattagtg
     6721 aatatgtatg tatcatttca attgcaatag caaaaggaat agcttcatct tcatttcctc
     6781 caactatttt acaagcctcc aatgtaagta ttggtcttaa tcttttacct ccagcactta
     6841 aactatagtt catagcttca ataacagttt tttgataccc ttcttcttta ggcatatatt
     6901 ctttaagtac tttttctaca aaacttgctt tttcttttag acattgttta aattccaatt
     6961 ttattcctcc tctattttaa agtcttcttc tattcctaat tgattaaatt tacttatttt
     7021 caatttagca tcatctaaaa gcttattaca atgtttataa aggcttatac cttcttcata
     7081 taggcttaaa gattcatcca agctagcatt tttagattct aattcactta aaatactttc
     7141 taatctttta taagcttctt cgtaggttaa attcatacta tccctcttta ttttcaattt
     7201 tatctatcat acattctaaa tttccatctt ttaatactat atctatacta tcctttattt
     7261 t
//
LOCUS       DAEPOZ010000188         7226 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.188, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000188 DAEPOZ010000000
VERSION     DAEPOZ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7226
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.188"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            259..534
                     /locus_tag="KR440_002916"
     CDS             259..534
                     /locus_tag="KR440_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0699927.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIHAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            790..2490
                     /locus_tag="KR440_002917"
     CDS             790..2490
                     /locus_tag="KR440_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0699928.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            2853..4040
                     /locus_tag="KR440_002918"
     CDS             2853..4040
                     /locus_tag="KR440_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699929.1"
                     /translation="MEAITAFCLVLISLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTIVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            4100..5263
                     /locus_tag="KR440_002919"
     CDS             4100..5263
                     /locus_tag="KR440_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0699930.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IEIPYVSAGGTGVIATIKGANPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAVLEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            5414..6217
                     /gene="zupT"
                     /locus_tag="KR440_002920"
     CDS             5414..6217
                     /gene="zupT"
                     /locus_tag="KR440_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBH0699931.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            6313..6873
                     /locus_tag="KR440_002921"
     CDS             6313..6873
                     /locus_tag="KR440_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HBH0699932.1"
                     /translation="MSAMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGI
                     FYFHKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSDSDKKNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVL
                     QIILLILYAVQLYRRLMYYLRLKKFK"
     gene            7124..>7226
                     /locus_tag="KR440_002922"
     CDS             7124..>7226
                     /locus_tag="KR440_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HBH0699933.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEI"
BASE COUNT         2734 a          755 c         1313 g         2424 t
ORIGIN      
        1 gtgatataga atataaattg aatattgaac agtttaattt aaatgattat aaaaatatat
       61 caaagtacat agagtcttgt ataaaaaatg aaattttaag tataagcaat gttgaaataa
      121 aaaacatatt aaaagaataa ttttaagatg ttcttcttat ataaaatgat ataattattt
      181 atataaataa agatataaga aatttatcaa ttttattttg agttataaaa tattaaagaa
      241 attgtaggag gacaatatat gaaaaaaata ttagtagcat gtggagcagg aatcgcaaca
      301 tcaacaatag tttgtgacag agtagaaaga ttggttaaag aaaataatat acacgcagaa
      361 gttgagcaat gtaaaattgc tgaatgttca acaaaacaag aaggtgctga tttaatcgta
      421 tctactacta tactaccaac aacttatgat atacctacaa taaaggcaac aggatatata
      481 acaggtataa atacaacagc attagataaa aagatattaa atgcattgaa gtagttttgt
      541 gtttataaaa aaaatttagt attaaatata gagttgtatc tttataaaat tttaaatata
      601 gagatatagc tctttttatg ctgtttcaag tatacaacta tatgatataa atcttcaatt
      661 ttatgaaaaa gattgttatt actttgtaag tttataaaag atttaaagtt attgttatgt
      721 tatactaatt tatgggtatt ttttagaagt actatataga agtactaata aaggagggat
      781 ttaatactta tgataaaaca atttgtcaaa tattataagc catataagaa aatttttaca
      841 ctagacctta tagcagcttt tctattttcc ttatgtgatt tagtttatcc tatgataact
      901 agaaatataa tggatgacgt tgttccaaat aaaaacttaa gaatgttagt agtttttgca
      961 gtagcactta tacttatatt tatagcaaaa gcagggctta actattttat gcagtattgg
     1021 ggacatgtta ttggtgtaga tatgcaagct gatatgagaa atgaggtatt tacacactta
     1081 caaagacttc caaatacgta ttttgataat aacaaatctg gagtaactat gtctagaata
     1141 gtaaatgatt taatggatat aacagaactt gcacatcatg gtcctgaaga tttattcatt
     1201 tctatagtaa tgttggttgg ttcattcttt atacttatag atataaatat tcctcttacg
     1261 cttataatat ttgctatatt accgtttata atttggtttg caatagctaa aaaagataag
     1321 atgaatattg cttttatgaa gagtagagtt actataggtg atgtaaatgc tactttagaa
     1381 aatagtatag caggtatgaa agtaactaag tctttttgta ctgagaaaga agagttaaat
     1441 aagtttgtaa gaagcaataa attatttaga agagcaagac aagattctta taaggtaatg
     1501 gcagaatatt actctggtat gaatttatac atggatatct tagagtgggt agttgtaata
     1561 gctggtggtt attttactta tataggaaaa attactttgg gtgattttgc tgcttatata
     1621 ctatatgtaa aaatgtttat acaacctatg aaaaaattaa taaattttac agagcaatat
     1681 caaaatggta tgacaggttt taaaagattc atagagataa tggaacaaga ccatcaaaaa
     1741 gaagctaaaa atcctattga acttgaaaat gtaaaaggtg acattgagat agaaaatata
     1801 tctttcacat atgaagataa gactcaagtt ttggataatt taagtttatc tataaaagct
     1861 ggtaagacta tagcattagt tggaccttct ggaggaggaa agactacatt atgtaatcta
     1921 ttgccaagat tttatgaatt tgataagggt gatataaaaa ttgatggaaa gagtataaaa
     1981 gatgttagtt taaaatcatt gagaaaaaat ataggtatag ttcaacaaga tgtattttta
     2041 tttacaggaa ctataagaga taatattctt tgtgggaacc ctaatgctac agatgaagaa
     2101 atgatagctg ctgctaaaaa agctagaata catgactttg tagagacttt gccagatgga
     2161 tatgatactt atataggaga aagaggcgta aaattatcag gtgggcaaaa acaaagaatt
     2221 tcaatttcaa gaatcttttt aaagaatcct ccaataataa tattggatga ggctacatct
     2281 gctttagaca atgtaacaga aagagaaatt caagagtctt tagaggaatt aagtaaagat
     2341 agaacaaatt tagttgttgc acatagattg actactataa aaaatgctga tgaaatcata
     2401 gtattgacgg ataaaggaat tgaagaaaga ggtactcatg aagagttagt aaacaaaaat
     2461 ggagtatata gtaggttaca caataactag tatacaaatt ttacaccata gctaacatac
     2521 gcattaaact tacaaacata ggatatatta agattcataa agacaattca ttttatattt
     2581 atgagatgtt ttatgaataa aatgtatcct atgttttatt ttttgcaaat tacatttaat
     2641 tctctttgta agaatagagt taaatagcca atggtcagga taacgttttt acaaaaagaa
     2701 taatttagaa aactaacttt aatttatgga atttatcgaa ttttatgaaa aaaatcgaat
     2761 tttgacttga aatgtttttc aaaagtagta aaataatgga taactataca tacattttat
     2821 aaaaatacat ttattatgag gggggatttt gtatggaggc aataacagct ttttgtctag
     2881 ttttaatatc actgacgata ggagatatag tttcagcaaa aactaaagct tttgttccgt
     2941 cggtttttgt atcagcaatt atatttatag taggattctg gacttttttc ccagaaaata
     3001 ttgtggactt agccgcttta ggaacaccat tagctcaatt aggtatgctt ttgttaataa
     3061 ctcatatggg aactatgatg agtattaagg aattagcagg tcaatggaaa acaatagtaa
     3121 tagccttggc aggaatagta ggaatatgtg taggtgcttt ggcattaggg acaatagtat
     3181 ttggatggga tacagcagta atagctacac ctcctcttac tggagggcta gttgcatcta
     3241 taatgatggc agatgctgct acagcaaaag gtcttgcaag tttatcagta cttgcaattt
     3301 tgatgtatgt tgcacaagga tttgcgggtt atcctattac agcattaatg cttaaaaaag
     3361 aaggaaaaag acttttatca gactttagaa gtggaaaagt aactgtaaat acagatgaag
     3421 aaaaagttaa ggatttacct gaacaaaaaa gcagatttaa gataattcca gacttaccag
     3481 aaaaatatga tacgacatat atgataatac ttagattagg atttgtagca tggttagcag
     3541 tacaatttac aaatcttaca aatgaagttg tatcaagata tgttatatgt ttaatatttg
     3601 gtgtaatagc atcagaactt ggaatagttg atagaaaacc acttaataaa tctggttcat
     3661 ttggatggtt aatgacatca ttgatggctt ttatatttgc aggattagca aaagctacac
     3721 cagcaatgct tgttgaaatg gcagttcctc ttgtaggaat cataatattt ggtgtaatag
     3781 gtatgggaat attctctatt atagtaggta agcttttagg tgaaagtaaa gaaatggctt
     3841 tttcagtttc gcttacatca ctttatggtt ttccaccaaa ctatatatta actgaagagg
     3901 cagtaaaggc tttggctgaa acaccagaag aaaaagaatt tttaatggat aagatgcttc
     3961 caaagatgct tgttggagga tttactacag ttacaatagc ttctgtagta atagcaggta
     4021 tctttgtaaa tctaatatag tatttaagtg ataaaaagta ttttaaataa gaagaatgtg
     4081 tactacttgg aggtatggaa tggatattaa agaaataaca aagagttata aagactatgt
     4141 aattaagctt agaagggaat ttcatgaaaa tcctgagaaa agtatggaag aagttagaac
     4201 atcaaagaga gttaaagaag aacttgataa aatagagata ccatatgtat cagctggagg
     4261 tacaggtgta attgcaacta taaaaggagc taatccagga aagactgtag cacttagggg
     4321 tgatatggat gctctacaag ttgttgaatg cactgatgta gaatacaaat ctaaaaatga
     4381 aggacttatg catgcttgtg gtcatgatgg tcacacatca atgcttctag gggcagcaaa
     4441 agtgttaaat gatattaagg atagtatcaa tggaactgta aaattatttt ttcagccagg
     4501 agaagaagtt ggtaaaggtg caagagctat gattcaagat ggtgctatgg aaggtgtaga
     4561 tagtgtgttt ggaatacatc tttggactga tgtagaatct gggactatat cggtagaaga
     4621 aggaccaaga atggcttctg ccgatttctt taaaattact gtaaaaggta gaggtggtca
     4681 tggttcactt cctcatcaag gtgttgatgc agtgcttgca agttcagcaa tagttatgaa
     4741 tctacaatct atggttagta gagaagtaag tcctctggaa ccattagttg tgagtgttgg
     4801 agttttaaat tctggaacta gatttaacgt aattgcgagt gaagctgtct tagaaggtac
     4861 aataaggcta tttaatccag agcttagaaa gcaaattcct ggaattttag aacgtatagc
     4921 aaaatctaca gcagaagcat atagagcaga tgctgaatta gagtatgggt acttaacacc
     4981 agcagttata aatgacaaag aatgttcaaa gatagctaca gatgcagcaa ttaagctatt
     5041 tggagaagac tgtataacac tatttgaaaa agtaacagga gcagaagatt tagctgagtt
     5101 tatgaatata gcacctggtg cactagcatt tgtaggagct agaaatgagt caaaaggtgc
     5161 ttgctatcct catcatcatg gatgctttaa tatagacgaa gatgctctag aaattggaac
     5221 tgctttatat gtgcaatatg cagtggattt cttaaataaa taattttaat atactataaa
     5281 gaaaaagcag agactaagtt tatatctctg cttttttatt gtgtatattt tttataaata
     5341 ttgactatat aaaatatata tgatagcata aagaaaatga taatcaatat caattaaaaa
     5401 gaaggtgatt tatttgaata atgtaatatt tgcatttttg ataacattat tggctggttt
     5461 atctactggt ataggaagtt gtatagcatt ttttgctaaa aaaacaaata ctaagtttct
     5521 ttctataagc ttaggatttt ctgctggtgt tatgatttat gtttctatga tagaaatttt
     5581 tcctaaagca caagatgctt tgacaaaatc tatgggagag aaattaggaa gttggtcaac
     5641 tgtaatagct ttttttatag gtatggctat cattgctgtt attgataagt taattccaca
     5701 agaagaaaat ccacatgaaa taaagaaaat ggagaatata gaagaaaaga atatcaaaaa
     5761 aaataagtct cttttgagaa caggtatatt tacagcaatg gctatagcaa ttcacaattt
     5821 tcctgaagga ttagctacat ttatatcagc tcttgatgat gttacaatag ctatcccgat
     5881 agcaattgca attgcaatac acaatatacc agaaggtata tctgtatctg taccagttta
     5941 ttatgccaca ggagataaaa agaaagcttt ttattattcc tttttatctg gtatgtctga
     6001 gcctttagga gcgattattg gatatgtact tcttagaaat tttttaaatg atataactct
     6061 tggaattgta tttgcaattg taggaggaat tatggtattc atatcactag atgaattatt
     6121 gccatcagct agagagtacg gggaacatca tttatctatt tatggattga ttgcaggaat
     6181 gggtgttatg gctattagtt tattactatt taaatagctt tttaaatata tgattgtaac
     6241 ctaaaattag tatcaaaatg atataattat ttaaatagat ttttaagcta tttaaaaata
     6301 tcattttgga gaatgagtgc tatggaaaag aaaataaaat tcaaatttga ctggtatagt
     6361 tcacctgata agatagagaa atggttagaa gatattgaag ctaaaggata taatttatat
     6421 aagataaata aatttggagg aatattttat tttcataagg gaagtccaag aaaaataaaa
     6481 tacactgttg attactacta tgatgtagag aataattatt ttgatgaaca taaatcaaat
     6541 ggctggaatc ttgtatttag gagtattggt agttttatgg gtacatctgc atggtatata
     6601 tggtcgaaag aatacaaaga catacctcct aaattatata gtgattctga taaaaaaaac
     6661 aaagtaaagt caattttatt tatgaatatg tttatattct ttatgttagt agtaatattt
     6721 atatatttat tgaatattga aataagaaat ttatatagtg gttatggagc atctgtaact
     6781 gctattgtac tacagattat attgttgatt ttatatgcag tacaattata taggagatta
     6841 atgtattatt taaggcttaa aaagtttaaa taaataaaag aaggtgtcct aaaattattt
     6901 ttttaatatt ttaagacatc ttcttttgtt agtaaagtac tgtttttaat tatagtaatt
     6961 tctgtcttta aattgaaata tcaaataata gttgtattgg attgagtatt gataaaaata
     7021 atttaaaaat gttatcctaa agtatcagat atacaaaatt atctttaaat atattataaa
     7081 tattttaatt gtattaacta gaaattatgt aaggaggcat tttatgatta agatagcagt
     7141 ttgtgaagat gaaaaagaaa cacaactttt aatagaagat taccttgaga atatattaaa
     7201 agatataagt atagaatatg agatac
//
LOCUS       DAEPOZ010000189         7135 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.189, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000189 DAEPOZ010000000
VERSION     DAEPOZ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7135
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.189"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            346..1197
                     /locus_tag="KR440_002923"
     CDS             346..1197
                     /locus_tag="KR440_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBH0699934.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            1201..1671
                     /locus_tag="KR440_002924"
     CDS             1201..1671
                     /locus_tag="KR440_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699935.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            1684..2994
                     /locus_tag="KR440_002925"
     CDS             1684..2994
                     /locus_tag="KR440_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0699936.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            3206..3952
                     /locus_tag="KR440_002926"
     CDS             3206..3952
                     /locus_tag="KR440_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0699937.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCNSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTADSI
                     SLLIDDGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            4314..5855
                     /locus_tag="KR440_002927"
     CDS             4314..5855
                     /locus_tag="KR440_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBH0699938.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKAGISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            5890..6996
                     /locus_tag="KR440_002928"
     CDS             5890..6996
                     /locus_tag="KR440_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBH0699939.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVTTEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFHGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DPLTTSNIGKIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHSMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
BASE COUNT         2529 a          950 c         1213 g         2443 t
ORIGIN      
        1 catttatttt aaaatttcaa tttactattt ctcaactcta tattattatt tttcaatttt
       61 atattactaa ttttaaagct ttttagctat tattcctaca atatttaaac aattttttca
      121 taataaagct atattacaat atattcaaaa tatgtaatat tttagatatt tttcgttgac
      181 aatgttaaat ttttgtttta tccttaatta aggacatgtt attgacattt ctgaaactta
      241 tttgaaatag ttttttcatt ttatttagga aaacgtatta tacttaagaa aacgtatttt
      301 ctcatttcag ataagtaaaa tatttattct tttggggagg aaatcatgga aattaataaa
      361 aaacatttta ttcaagtatt tttaacttgc ttaatcttgg tagtaatatg tgaatttata
      421 gggcaaagaa ctatagctat tggaaaagcc tctatagtac tgttccctat gctttatgca
      481 gttataattg gacttataat aacaccagat ttattaggta aaaaaattaa agcacttaaa
      541 aaagtggttg gagaaaaaga aataaatatt gctggtgaag ttgttggaat tgcactagta
      601 atgcttggta taaaatatgg tactacagtt ggaccaaaca ttgacaaaat aattcaagct
      661 ggaccagcat ttatcgctca agaatttggt catatacttg caccaatcgt tgcattacct
      721 cttgcattaa tgtttggaat gaaaagagaa gctgttggag ctactgcaag tataagtcgt
      781 gaaccttctt taggagttat atcagaaaaa tatgggataa actcaccaga aggtagtggt
      841 gtacttggta catatttaat gggaacagtt cttggtacta tatactttag tgtactaggt
      901 tcaatatcta tttatagtgg tcttcaccca tatgctttgg gaatggcctg tggtgttggt
      961 agtggaagta tgatgacagc agctgctggt tcattatcta ccatggtacc acctgaaatg
     1021 gcagatacaa tacttgccta tgctgcaaca agcaatatga tgtctagtat aacaggaatt
     1081 atattcttag tttttgtatc cttaccattt actaatttta tgtataagat tttagaacct
     1141 aaattttcga gaaataaaaa aactaaagaa attaaatcta cagaaggaga gtgctaataa
     1201 atgtttgcta atttaaattt aaagaaaagt tcatatcaat tattggttat gtctgttatt
     1261 ggagttataa ttcttatagg acaaagaatt agcgttggta catctatagt tacagctctt
     1321 cctggtatgg tgatgctaat attagctgct atggcagcta tgattataaa agatttattt
     1381 cctaaatcaa tctttccagc ttttggattt gcaactataa tcggcctttt acttagtatg
     1441 ccatatagtc caacatctga agtattctta actaacacta ataatattaa ctttatggct
     1501 ataactactc cactactagc ttttgcagga atttctgttg gaaacaaaat agaagcttta
     1561 aaagaaatgt catggaaaat agttataatt tcacttattg tatttacaac tatattcttt
     1621 gcatgtgcga gtatagctca tatagtttta agtattcaag gtaaaatata ggaggaattt
     1681 attatgaata aagaacaatt aaaatctcta gtaataaaaa ctatagatga aaatagagaa
     1741 aaaatattaa aaataggtca aggaatatac aaaaatccag aatatggata taaagaattt
     1801 aaaactacag aagctgtttc aaactttttt gagcaagaat taaatttatc tgtagaaaaa
     1861 aatatagcag ttacaggatg caaagcagat gcaaattcta gcaagaaagg tcctcatata
     1921 tctatacttg gagaattgga tggtatatca tgtaaagaac ataaagatgc taatgaaata
     1981 ggtgcttctc atacttgtgg acataatatt caaatagctg gaatgttagg tgctgctgtt
     2041 ggtttagtaa aaagtggcgt tttagaacat ttagatggta aagtatcttt tatggctact
     2101 cctgctgaag agtttattga gttggaatac agagagcaat taaaaaaaga aggaaaaata
     2161 acttattttg gtggaaaaca agaattaata aaaagaggtt attttgatga tatagacata
     2221 tctatgatgt ttcatgcttc tgatttagga gaagataagg ctctagttgg tcctgagagt
     2281 aatggatttg ttggaaaaaa ggttaaattt ataggtaaag aatctcatgc tggttctgct
     2341 ccacatgatg gaataaatgc tttaaatgca gcaatgcttg ctataaacaa cgtgaatgca
     2401 cttagagaaa cttttaaaga gtctgaaaga gttagattcc accctatcat cacaaaaggt
     2461 ggagatatag taaatgttgt tcctgctgat gttcatatgg aatcatatgt tagagctaga
     2521 actataaatg gtatgattga tgctaatgaa agaataaata aggctttgat ggctggtgga
     2581 atggcagttg gagctgatgt tgagataact gaaattcctg gatatcttcc aatattacga
     2641 tacaaaagtc ttgataattt atttaaaaac aatcttgaag atttaggtct aaaaggaaaa
     2701 gttattgatg gtggagattt tacaggctct tttgattttg gagatgtgtc tcatataatg
     2761 cctactcttc accctatgat tggtggtgta aaaggagctc ttcatacaag agattttgaa
     2821 atagttgatg aagatttagc ttacataatt cctgctaaag caatggcact tacagttgta
     2881 gaccttcttt ttgataatgc taaggatgct aaagatatat tagatacctt tactccagtt
     2941 atgactaaag aagaatatct aacattccta gaaaaacacg ataaaacttt ataagtctat
     3001 ttttataata gggtggtatc tactgtaaaa gttataccac tctttgtttt tgtaacagta
     3061 tacttctttt cttttagctt ttagattaaa ctaaaatata ttatcctcct taaaactttt
     3121 agagatacgt taaaatatca gtagaactat gctatattct ttctaattaa tataaattta
     3181 tattctaatt caaggtggtg aaaaaatgtt taatgcagga aatcgaatta taaatacatg
     3241 gttgtaccct attcaagatg gctttgtctt gattgatact ggatatgaaa atggttttaa
     3301 acatttcaaa aaacgaattt ctaaattcaa cataaaaatc aaagatattc gatacatatt
     3361 tctaactcat gctcatgatg accatgttgg ttttctaaat cagattctag acgaatctcc
     3421 taatacaaag attgttatgc atgataaagc tttagaaatt ctatataaag gtcaaaactc
     3481 ttttattggt ggttgtaaca gccgtactgc ctttctcttt tgccaaatta tgaaactatt
     3541 tggaaaaggt gagcatagtt tttctccact taaacaagaa tttgaaaaaa ggtgtattct
     3601 tgtgtctgac aagaatcgta aagaaattga aaaagaacta aatggaaaaa ttatagatac
     3661 accaggacat actgctgatt caatttcact actaattgat gatggtgttt tattttgtgg
     3721 agattctgca atgaatggat ttccaagtac tcacaaaatt accatatttg cagaaagcaa
     3781 actagaattt attcaatctt ggaaaacaat catcaataca aaacctaaaa tgatttatcc
     3841 tggacatgga aaaccatttg aatacagtaa attagaacaa aatctaatct atgtaaataa
     3901 aataaattta tatcctctac ataattcaaa agatattcac agatttttgt agaaaataca
     3961 aagtaaattt ctttgattaa ttaaaaaatc taacacttta ctacgctact tattttaata
     4021 acttaatctt ccactagctt ttcataaaat tttgttttaa aattaacttt aaagtgcata
     4081 tgctatttaa atacattaga tataattcgt cattaatcta cttaaagcat atttaactaa
     4141 caccactcaa ccagaaaatt cagaaaactg cattgacaca ggaaaaagtt tattttatac
     4201 tttttttagg acatattgtg gacacgtctt aaatcgacaa gcagaattta tattgtaaga
     4261 tcaaccacaa tacttatttt ttaattatat ttttaaacat tagggaggat attatgaatg
     4321 ataacgtagt taagaagaaa aacttttttg acaggacatt aaacagaata gaaactgttg
     4381 gtaataaact accagaccca gttacaatat ttttagggtt atgtgtactt ttacttatac
     4441 tatctagtct agttggttct atggggatat cagtagttca tcctggtact ggagaaacta
     4501 taactgcagt aaatttattg actgtagaac agcttcaaat tcttttagga aacatagtta
     4561 gcaatttcca agggtttgct cctttgggtc tagttctagt aactatgata ggtgctggtg
     4621 tatgtgacaa aacaggactt atgacagcaa caatcaaagc atcagtaagc aagattcctg
     4681 aaacaagagt aactcttgtt gtaatgacta taggtatgct tgccaatata gcttcagatg
     4741 caggtacaat attatttcca ccattagctg cacttgtata tcttggtgtt ggtagacatc
     4801 cacttatagg tttattttct ggatatgcag cagtctgtct aggatttgcc gcaaacatta
     4861 tgataagtgt caatgatata cttgctgcaa gttttacagt tccagcagct caaatgattg
     4921 attctggatt tcaagccaat gcaacaatga acttattttt tatgatagca tcaacttttg
     4981 ttctaatact tttggcaaca tgggtaacag aaaaaattgt agctcctaga tttggtaaat
     5041 atgaaggaaa tgcagaacta gatgttgatg gaagtcttag tgatgtagaa aagaaagggc
     5101 ttaaaaaagc tggtataagt atattaatat atgtagctat tatagtagct cttagtgtaa
     5161 tcggtaaaaa accattctta gctgacccag aaacaggaga tttactatca aacaatgctc
     5221 ctcttatgaa aggtatggta cctataatag cactagcatt tttcatacct ggtgttgtat
     5281 acggaaaaac tataggtaaa ataaagaagg ataaggatgt agtctctata atggctagtg
     5341 caatgagtga tatgggtgga tatataatac tagcatttgc tgcttcacaa tttttgcaat
     5401 tattcacaaa tagtaattta ggtctgatac tagctgtaaa gggcggagaa tttttaaaat
     5461 cagcaggaat aaatggacct ttattgttga taggatttat tatattatca tgttttataa
     5521 atttatttat aggtagtgca tctgcaaagt gggctattct tgcacctata tttgtaccaa
     5581 tgtttatgat ggttggatac aatccagcac ttactcagat ggcttatcgt ataggtgatt
     5641 gtataacaaa tccacttagc ccattatttc cgtacttccc tattatacta gcattcacaa
     5701 gaaaatacga taaagatgct ggtatgggta ctgtaattgc taatatgata ccatactcta
     5761 tatcattctt aatagtgtgg actatactat taatattgtt tatggtattt aatattccac
     5821 taggacctgg aatacttcct tcatatagca tgtaatttat ttaaatcttt ctaaaaggag
     5881 aaataattta tgttagataa aaatagactt ataaataatt ttatggatat ggttagaatt
     5941 gatagcccat ctaatcaaga attagaaatg tctaagtggt tggtaaacta cttaaaagaa
     6001 agaaatatag atgccattat agatgatgca ggtgaaaaat atggtggtaa tacaggtaat
     6061 gtcattgcct atataaaagg tgaagaagga agtagaccac tttgtttatg tgcacacatg
     6121 gaccaagttc aaccttgttt aggtgtaaaa ccaatactag atggtaatgt agttagaagt
     6181 gatggaacta ctacacttgg tgcagatgat aaagctggaa tagctgctat acttgaagct
     6241 ttagaacatg ttactacaga aaaaatacca catagagata tttatctttg ttttacaatc
     6301 tgtgaggaag ctggtatgca tggtgtcaaa aactttaatc ctgacaactt gccttgtaaa
     6361 gatatggtga ttttagattc tggtggagct attggttcga tagcatacaa agctcctgct
     6421 caacaatcta taaaaatctc ttttcatgga aagaaagcac atgctggtat agaacctgaa
     6481 aaagggttaa atgctatatt agtagcctct catgcaataa gcaatatgca cataggcaga
     6541 atagatccat tgactacttc aaatataggt aaaatagaag gtggtggagc tacaaacata
     6601 gtaactgata aggttacatt gactgctgaa attaggtctc atatacctga aactttagag
     6661 tatgagctta atcatatgga aaaatgttgt aaggatgctg catctaaatt taatactact
     6721 tatacctttg aacatagtat gtcttaccct agttttgaac ttagtagaga tagtcacgtt
     6781 ttcaagttaa gtgaagaagc aataagacaa gttggtgttg ttccaaatcc aatggtaatt
     6841 ggtggtggaa gtgacgcaaa tatattagct aatcttggat ataattgtgc tatattaagt
     6901 ctaggtatgt atgatgtaca cacagttaat gaatatgtaa atatagacga attatatgat
     6961 actgccaaaa tagtttatca tatgattaaa ttataaatat cataatatac ctctttaaat
     7021 tataaaaata aaaacctatg tatcttcaac tttataagtt atatattaaa gttgttttac
     7081 ataggtttta gtatcttata taataattta ttttaacatt tttcataata agctt
//
LOCUS       DAEPOZ010000190         7077 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.190, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000190 DAEPOZ010000000
VERSION     DAEPOZ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7077
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.190"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..569)
                     /locus_tag="KR440_002929"
     CDS             complement(<1..569)
                     /locus_tag="KR440_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBH0699940.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVT"
     gene            complement(557..1429)
                     /locus_tag="KR440_002930"
     CDS             complement(557..1429)
                     /locus_tag="KR440_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0699941.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDREKFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVRGCSI"
     gene            1564..2364
                     /locus_tag="KR440_002931"
     CDS             1564..2364
                     /locus_tag="KR440_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HBH0699942.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            2389..2958
                     /gene="yfcE"
                     /locus_tag="KR440_002932"
     CDS             2389..2958
                     /gene="yfcE"
                     /locus_tag="KR440_002932"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBH0699943.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
     gene            complement(3376..4428)
                     /locus_tag="KR440_002933"
     CDS             complement(3376..4428)
                     /locus_tag="KR440_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HBH0699944.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
     gene            complement(4635..5861)
                     /gene="pepT"
                     /locus_tag="KR440_002934"
     CDS             complement(4635..5861)
                     /gene="pepT"
                     /locus_tag="KR440_002934"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBH0699945.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCREKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            complement(5952..7003)
                     /gene="ytvI"
                     /locus_tag="KR440_002935"
                     /pseudo
     CDS             complement(5952..7003)
                     /gene="ytvI"
                     /locus_tag="KR440_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
BASE COUNT         2447 a         1174 c          787 g         2669 t
ORIGIN      
        1 tcagttacat aatttgaaag atatactcct ccaactttac tataattaaa atgctttact
       61 tgatatacaa tcatactaat catcttcaaa agacttccat atacaaagta aattaaaata
      121 cttactataa aaccacaaac tttcattttt ccatatccaa acttaaaaat taaaggcagt
      181 aaaatcgtca aaattgctaa aaatatattt acagaaaaaa atacatcatt taaaaccata
      241 tttctaaaga acactagtga tgtcaattta cttagtaagg acacaattat tgttacagaa
      301 attattagtc ctacacctaa taaatatttt gagtaaacta tatcttctat actaactggc
      361 atactcctta taaaaatcct tgaatcattt tcataatcac actcaaatgt atttataaat
      421 atcaaataac taacaacaat acttactgta taatatgaaa ttgaattaag gaaaaaataa
      481 aacacaaaaa atagcaatat gtactttaaa ttacggatac catctgaact aattcctatt
      541 attaaatctt ttcttattaa attgaacatc ctctcacctc atttacataa taatacatta
      601 gttcttctat agtaggattt ttgaccttat gcttatcaat ttctatcaat aggcttttta
      661 ctttcaagct atctccaact ttaacaagtg cctcattata atatttatac tctttaacac
      721 caactatact ttctttaatt gcttctaact ctgctttatt tgccctaatt atcttatatt
      781 cttgttctat taattctttc ttaacagtaa aaatctgatt tcctctattt ataaaaacaa
      841 gataatcagc aattttaatt aaattatcag tattatgagt agaaattatt acactactac
      901 ttccattttt cacaaaatct tgtaagtact gcattatttc agttcttaca atcgggtcta
      961 taccagcagt tggttcatcc aaaatcaata acttagcatt atgactaagt gcgcttgata
     1021 acataagctt ctcagattga cctttagata aattttcaat atgcatattt tcttctatgt
     1081 caaacttatt aaggtattca tcaaattttt ctctatcaaa attcttataa aataaagaaa
     1141 ctatttttct aaattcctta acctttaaat gtggataaaa ttgtaaagaa tcatatacaa
     1201 aaccaatatt gtttttaaag cttattggag aattttcaat agtttcatta caaagttcaa
     1261 tttcaccttc atctgcatca ataatcccca ttataatttt tattaaagtc gtttttcccg
     1321 aaccatttgg tcctataatc ccaacaatat ccccttcctt taactctaag tttatattat
     1381 ttaacttgaa ttttcctaat ttttttgtga tattccttat gctaaacaca tcttcacctc
     1441 ttatgcattt ttttataccc acctatacat attttaacac attcactaca aaggtcattt
     1501 ataaatttat aaattaagaa ttataaaact atgttataat acaaagtatg aagaggtgaa
     1561 tatatgaaaa gaattataac tataaataca aaccaattaa ataagtccct tgagactaaa
     1621 gaactcttaa ctagaaaact tatcaatgct ggctttgaag tgtattcaga tatttatcca
     1681 gacacagagc ttattatatc aattggtggt gatggctctt ttttaagaac agttagagat
     1741 ttcgattttc ctgagattcc cataatggga ataaatacag gtcatctggg gttttttcct
     1801 gatattttac cagataagat tgatagcttt attgaagctt atacaaaaaa agattatatt
     1861 attcaagaaa tgtcacttct taatgcggaa gtatatacaa ctactagtgg ttctaatatg
     1921 cttgctgtaa atgaggttgt tataagagga gacaagtcta ggactataca cctaaattta
     1981 agccttgata ataaacatat acaaaatttt agtggagatg gtatgattat ttctacttcc
     2041 acaggttcaa cagcctataa ctattctgct ggaggtagta ttgttgatat aaatcttgaa
     2101 cttatgcaga ttacaccttt acatccgata aataccaatg catataggtg ctttacatca
     2161 agtataatat gttcaaatga atctgttata aagattgcac cagaatatag atttgaagat
     2221 tctgtattga ttgtcgttga tggagttgag cacagattta gacaaatcga aaatataaaa
     2281 gttagtatat ctgatgcaaa aataaaatta cttagaatgt caaactatga gttttggcat
     2341 agagtatctg aaaaattttt atagactgta cttaggagga tttttaatat gaaaatagga
     2401 ataatgagtg atactcatgg tagcttactt tactttgaaa aagcgttaaa tgtattatct
     2461 gattgtgatg ttttattaca tggtggagat gtcttatatc atggacctag aaatgatatt
     2521 ccagaaggat ataatcctaa aaaatttatt gagacattaa ataaattaga aaacattgtg
     2581 attgtaaaag ggaactgtga tgcagatgtt gaccagatgg ttatagaaca tcctattcag
     2641 agtccttatg tcatgagcca atttggtgaa attagaatta tattgaatca tggttatata
     2701 gaatcagaag aagaaattat tgacaaagca aagaaaatgg gtggtgacat attagtttta
     2761 ggacatactc atgtaaaaaa actatacatg gatgataatt taatagtaat aaatccagga
     2821 agtacttcta tcccaaaaga tggaagccat tctgtcgcaa ttatagatat cataaaaact
     2881 gatgatgagg atgagcttga acttgatata aacttaattg acatcaatac tggaaatata
     2941 ataaatatca atgattagta aatttttaat aaaaataaaa taagctttaa tttattaaac
     3001 tcataataaa aacaagtaaa ccattttatt tctatctcaa ttagaagttt aagtacaata
     3061 ccttaaactt ctaattaaat agaatctggt ttacttgttt tattatatgc tatattgaaa
     3121 tataacttag tttacttgtt tctattatat attatattgg agtataactt agtttactta
     3181 tttcattata tattatactg aaatataact tagtttactt attttattat atattatatt
     3241 taaacatagc ttagtttact tatttcatta tatattatac tgaaatataa cttagtttac
     3301 ttattttatt atatatattg aagttcaact tggtttactt gctattatta tatattaatt
     3361 ttgatttaca caatattact gtaaggctaa tgcttctgca tatggtttac tatttttacc
     3421 tccaccaact tgtacaaatt tatcagcctt tacttttttt ataaaacttt tctgtgcact
     3481 acttaaactt ccacttgcaa gtactacagg tgactttgtt tttgatgcta atactccagc
     3541 agcaagccca tcaactaaat cagcttctct accacttcca tcttttgcaa aatacatatt
     3601 attaacctta cttgattttc cataaaactt ttctaaaact ttcgcattag tatcttttct
     3661 atctgttcct gctagtcttt ctgagtttac taactgtcct tcaagttttt tacttatact
     3721 gtactcccca cctactatgt atgtcttagt tgagttttgt gtaataaaat tctttgcctc
     3781 acttcttatg ctatccttac ctgtatatat tatagctgta ttatcacttg cagctgggct
     3841 tgatattgaa gttgcatctg ccagtccatt atacccatta accactgcca cttttgagac
     3901 acctaaatca ccagtatcat ctagctgtct agtaagaagt aaagatgttt cttctctatc
     3961 tgctccaccg attctactga ctaatacttc catatcttca agagattctt ttacacttga
     4021 tgaaatagct ccttcacttc caattaaaat aacttctgct ggattaagcc tttttatttc
     4081 ctctgttaca ttatatggta tactattttt atttgtaagc agtattggtg catccaattt
     4141 acttgccaaa ggagttgaag ccaatgcatc tggcaatgaa tttgagttaa ctagaaacac
     4201 cctttctgaa ccattattcc aaccactttg agatattttt atcgctgtat caaatctatc
     4261 ttcaccaata agtttttcct cctttggaga ttttctacct tgaagataat tgttcatatc
     4321 tacctgtgca ttttgatttt tactaatagt gtctgcatat acacaacttg gcaacataat
     4381 cccaagtgaa agtgcaaatg ctattatttt tttagattta attttcaata caattccccc
     4441 ttttaaatgt taatacctat tgatatccat actttttatt gataccctaa actaaaaata
     4501 attatctttt ataaaaataa ttacaaaaat agctatacca ttttaaatac aacacaagct
     4561 tataatcagc ttgacttata aagaatgata tagctattat aactttaaat gcttaatttt
     4621 taaatacttt ttaactaaac tctttcagca tatttttcag ctattcttac aattagttta
     4681 gtagctttct ccatagaact aactggtata cactcatttt tcccatggaa gtttaatcca
     4741 ccagtaaata tatttggaca aggtaatcca ttaaaagata atcttgctcc atcagtacca
     4801 cctcttacag gaactaaacg tggttttata cctaattcct ccatagcttc tttagctata
     4861 tcaactataa acataactgg ctcaatcttt tctttcatat tgtaataaga atcttttaaa
     4921 tccaattcca ctctattatc gtatttctta tttatttttt ctatattctc tatcataata
     4981 gattttctat tttcaaattt ttctctacaa tggtctctaa taatataagc catacttaca
     5041 ttctcgacat ttccacctat agaatttaaa tgccaaaatc cttcatatcc ttctgttgtt
     5101 tctggtcttt catctgctgg gaacatttct gatatttcag cagctatatg tatagcattt
     5161 accattttat ttttagctga acctggatga acatttctac catgtatagt aatagttgca
     5221 tttgcagcat taaaattttc acattcaagt tctccaacta ttcctccatc taaagtatat
     5281 gcatattttg ctccaaattt ttcaacatca aataaatcag cacctctacc aatttcttca
     5341 tctggtgtaa atcctatttt tatatctcca tgcttaattt ctggatgatt tattagatac
     5401 tctattgcag tcactatctc agctatacca gctttatcat cagagccaag tagagttgtt
     5461 ccatcagtga ctattaaatc gtcaccttta aattcttcca actctggaga atcctttaca
     5521 tacgtaataa catttaacgc ttcatttaaa actatgtctt ttccgtcata attttctatt
     5581 attcttggtt taatatcttt tccactaaca tcttcaattg tatctaaatg tgatatgaat
     5641 cctatagtat ccacaccatc agtatttcct tttaaagtag ccattatata actattttca
     5701 tctaaactca catcatcaac acccattgtt tttagctctt caactatata cttagctaaa
     5761 actctttgac cttctgaaga tggacagttc tcacactgag aattagatgt tgtatcaaat
     5821 gatacatatt ttaaaaatct ttccactact ttttgtttca cgtatatacc ctctttctta
     5881 ataagattaa tattttttta tttacctgtg aataaaattt tatttttaaa aaaatctatc
     5941 aattattata attaaaaaat atccaccttc ttaagtatat catgcataca taccaaaccc
     6001 aaacaaaaaa ttactccaat tattcctttt acttggattc caataaaaat cgctgctagg
     6061 gtagctaatg gatttagtcc aacatttact gatactagtt taggttcaag tatttctcta
     6121 atcaaagaca aaacaaaaaa tatcacagtc atacttatag ctataaaata attttttata
     6181 agaaaataat aaacaattac tggaagatat attgttatta tacctaggaa tggaattaaa
     6241 tccattaaag ccctacaaac cctactatta gaccgtattt caatccaaat aaagcaaaac
     6301 ttccccagat tgcaaaaaat gttatagcca taagtattgt ataggccctt atatatccaa
     6361 caagtgaaag ccctgcctct ttctttacgt tttttacctt ctttctaaca tcaacagtaa
     6421 acatattata aaaactattt tcaatcttat caaggtcttt tgctataaag taggtagcaa
     6481 taaacaaggt aattataaat attataatat atggaataga agtagccaat ccaagtatac
     6541 tagtaagaag attttttgta atttgcatta cttcaccact gaatttgttt actaattgtt
     6601 ctacatcaaa attggctatt tcttgaaatg atttcataaa gtcatttatt ttatttaaaa
     6661 aatacataat agtattactt atatcctcag gattagcaga tatattattt aaaaatccca
     6721 ttaattcttt cattgaattt attactatta ttgtagtaac tgtactgact atagccactg
     6781 caagaaaact tagaacaaga gtagagatac ctttatttat acgaaactta ttttctaact
     6841 tttgtgatat aggattaatc ataagtgcta ttatagcacc tagaaaaaat ggagctatgt
     6901 atggaaacgc tttgtaaata aaaataaata acaatgtata tatacaaaaa aggatagtgt
     6961 tgttttttaa tttgtataaa aaattatttt ctagtatgct catcttaaaa atctcctttc
     7021 ttctaattta tatttttatt ttgtccattt catactagtt ttatttgaat acaaaat
//
LOCUS       DAEPOZ010000191         6943 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.191, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000191 DAEPOZ010000000
VERSION     DAEPOZ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6943
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.191"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            230..1309
                     /locus_tag="KR440_002936"
     CDS             230..1309
                     /locus_tag="KR440_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBH0699946.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDEINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIAKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYMKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRRH
                     YPGIHSITIIINGVEKGKLDFELGV"
     gene            1633..2001
                     /locus_tag="KR440_002937"
     CDS             1633..2001
                     /locus_tag="KR440_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBH0699947.1"
                     /translation="MDVYSFWNDTLAQDAVKIRKYFHEDAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVERVLEFGNLVITVTHVYAINKPLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            2118..2921
                     /locus_tag="KR440_002938"
     CDS             2118..2921
                     /locus_tag="KR440_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0699948.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDKQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKLLRNIISK
                     MCGATSEGRRSMMKMLEGYEKKELCHLMYLGFAGFMPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPSELNQIMDIFIKQLNI"
     gene            3306..4196
                     /locus_tag="KR440_002939"
     CDS             3306..4196
                     /locus_tag="KR440_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH0699949.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSARFDAVKELNDSGIFCGILLTPMLPFLTDNKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVLKYQKTYGLNYVCTIQNKDSCEKLLREECARYGILTD
                     MKEIIKAYKRSESYIQIKFF"
     gene            4659..5102
                     /locus_tag="KR440_002940"
     CDS             4659..5102
                     /locus_tag="KR440_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0699950.1"
                     /translation="MIEIKTIEINKKSQIDLLMLADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
     gene            5274..5444
                     /locus_tag="KR440_002941"
     CDS             5274..5444
                     /locus_tag="KR440_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699951.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            5995..6903
                     /locus_tag="KR440_002942"
     CDS             5995..6903
                     /locus_tag="KR440_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBH0699952.1"
                     /translation="MTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFINDI
                     LKDAPYANRNIKPFTHIKRIIKAETDPTINYIGEKRPRLYILAEDEESNHINIEMQRA
                     LEDDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLTM
                     DGHPNIYSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIKR
                     ALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGVEKGKREEKFNIIIKLKNK
                     GYNLSEICDIIDDLNKSEVEKIYNQN"
BASE COUNT         2703 a          716 c         1244 g         2280 t
ORIGIN      
        1 agtaaagtat aaaattctat tactataata aaagatagaa ctagaagtct ttatgaaagt
       61 gggaaaagta tttatataaa taaatttata tggtttatag tttaacagta ggaaatatcg
      121 tatgagtgag aatatcgtat tttacataaa gatttagcaa tatgacacac tgttgtcgtg
      181 ttctgtagga tatgattaat aaatcagata tgaaaggaga gtgagtaata tgccagaatt
      241 actaaaaaat atgtataatc gtgagtcact ttatgaagtt gcagtagcta tccaatcagt
      301 ttacaactca tttaaggtag acgagttcat aaaatctaca atggatgaga catggaataa
      361 cttagaatta aaagccagat gtcgaaagat aagtatgagt ttaggaatgt atttaccaga
      421 agactataag gaagctcttt ccattcttga aaaatcagtt acaggttttt attttgcttt
      481 tttctttcca gattttgttg aagtttatgg gcaggacgaa ataaattggg atttatctat
      541 aagtgcatta gaaagaaata ctgagtattg gtcttcagaa tttgctgtaa gagcatttat
      601 catcaaggat gaagagcgta tgatggcaca gatgcgtaaa tggtctaagc ataagagcga
      661 acatgtacga agacttgcaa gtgaaggttg tcgtccccag ttgccttggg gacaagcaat
      721 atccaaattt aaaaaagacc ctacacctgt actgcctata ttggagcaac ttaaaacaga
      781 tacatcaacg tatgtacaaa agagtgttgc taataatcta aatgatatct caaaaacaca
      841 tccagattta gtcataagca ttgcaaagga ttggtacggt aaaaataaat ctactaactg
      901 gattgtaaag catggttgta gaactctact aaaaaaaggc aatcgtgatg tattagccct
      961 ttttggatat gatgatacta cttctataaa tcttcaggac tttactttag aaacaacgtc
     1021 tatttctatt ggagaggatt taactttttc atttaatatt ttagcaaaaa aagcgacaaa
     1081 aacaaggctc gagtacggaa ttgattatat gaaatctaat ggaaaaagga ataggaagat
     1141 atttaaaata tcggaggttt cattaaaaga aaatgagaaa aagtcatata tgaaaaaaca
     1201 ttcatttgca gatgtaagtg taaggagaca ttatccaggt atccactcga ttactattat
     1261 tattaatggg gtagaaaaag gcaaacttga ttttgaatta ggtgtataat tttactaaca
     1321 gcataaccta tatcagatac tttgctaaac ctatatcgta tactctaata aatctatatt
     1381 aggtagttta ctacttgttt actgaattta aatacaaaga aatacaggtg ttaatccctg
     1441 cccattaaag gaatatgagc ataacacaaa agtagaactt atcaattctg ttacgggtat
     1501 agtagtaaat ccatagaatc acaattagaa gatgaagtgt aaaaaataag ttagtgttga
     1561 ttttagttgc tagtgatatt gaatagttat acaatagcca tataaattag aatttgtaga
     1621 ggagaaatgt aaatggatgt atatagtttt tggaatgaca ctttggcaca agatgctgtt
     1681 aagataagaa aatattttca tgaagatgct tatattaatt ggcatgatac aaatgagcat
     1741 tttactttag aagaatttat tattgcaaat tgtgagtatc ctgataaatg gaatggtaaa
     1801 gtagaaaggg tgctagaatt tggaaattta gtaattacag tgacacatgt ttatgcaata
     1861 aataaaccac tatcatttca tgtcgtttca tttatcagcc ttaaagatga taagattatt
     1921 tctattgatg aatattgggg agaggatgat gatgtgccac agtggagatt agataaacat
     1981 attggaaagc caataaaata gtgtttgttc tgttttataa ctgaaatttt gataacattt
     2041 gatattttga tttgtcattg gtcaacatag tcgttagtgt aataatttaa aaaagtagaa
     2101 tttggagagt gagcactatg gaagaaaaat acatcttatc tgaccgaggt aaagtatatt
     2161 attggataaa agaaagtaat agtgtgtttg caaagaactt aatttttttg ccaggactaa
     2221 cagcaaatca tcatcttttt gatttccaga tacagtactt ttcaaaaaat cataatgtat
     2281 tggtatggga cgctcctgct catggaaaat caagacctta cttagatttc tcatattcta
     2341 acttggtaga agaattgaaa gtaattttag ataaacaaaa tatgaaaaag gttgtactta
     2401 tagggcaatc ggcaggggga tttgtggcac agtcctttat tgtaaaatat ccaaatatgg
     2461 tagaaggatt aatgatgatt ggaacttgtc catatggaac tgactattat agtaagtcag
     2521 atattttctg gttaaaacag actgaatgga tgttaaagtt attcccagat aaattgttgc
     2581 gaaacataat atctaagatg tgtggagcta cgtctgaagg cagaaggagt atgatgaaaa
     2641 tgttagaagg ctacgaaaaa aaagaactgt gtcatctcat gtatctggga tttgctgggt
     2701 tcatgccaga aattcaagac ttaaatatac attgtccagt ttgtttggtg gttggagata
     2761 aagacaagac gggtaaagtg agaaaatata atgagcagtg gcacaataag actgggtatc
     2821 cattgcacat tataaaaaat gcttcacata atgtaaatgt cgataaacct agtgaactaa
     2881 atcagattat ggatatattt attaagcaat taaatatata aaaactacat actcattagg
     2941 taaattatgt agtatatgtg tttacatatg gttatagaaa taaatatgta ataaactact
     3001 taacacttaa aaagtggatt tgtccagagc ataatacaaa gcatgatata taaattttta
     3061 agcgaaatac atcaaaagaa gtattagaat tagtggagat aaaacagcta ttagagatta
     3121 taaataaata gttttaaagg tactacgaat atagaggaag gtaaatcata cgttattcaa
     3181 ggattataaa tgagtagttt aaaagtacta tcggataggt ctctgcatat ttaaacctta
     3241 gtaaaggtag ttaaagtagt agtttaaaaa ctaaaaaatt atataaagga attaggtata
     3301 agaaaatgat tgagagtata ccagcaaaac aaattttaca aaaagtgaaa tttgacaata
     3361 caagatggtt tggaattgac tataatatga atctctatcg tggttgttct catggatgta
     3421 tatattgtga tagccgtagc acaatataca acatagagaa ttttgacaaa gttagatata
     3481 aagaaaatgt gatagagatt ttaagtaaag aattgcgttc aaaaagaaaa aaaggggtag
     3541 taggcattgg agctatgtct gacacatata atccttttga aaagcaactt tgtattacaa
     3601 agcaagcact agatttgatt tcggaaaatc attttggagt gtctattgat actaaaagta
     3661 gtttggtggt tagagatata ccgattttgc agaaaattaa aaaaaataat tctgctattg
     3721 taaagttgac tataaccact gcaaatgatg aattatcaaa gaaaatagaa ccttatgtaa
     3781 atccaagttc tgcacgattt gacgcagtga aagaacttaa tgattctgga attttttgtg
     3841 gcatattatt gactcctatg cttccatttt tgactgataa taaagatgaa atacgagcaa
     3901 ttgtagaaaa agcacacaag gctaatgcaa agtttattta ttgcatgtat ggtgtaacaa
     3961 tgcgtagtgg tcagagagag tttttttatg agcatttaag agatatatca ccaaaattag
     4021 tacttaaata tcagaaaaca tatggtttaa actatgtatg tactatacaa aataaagatt
     4081 cttgtgagaa gttgttaaga gaagaatgtg cacgttatgg tattttgaca gatatgaaag
     4141 agattataaa agcgtacaag aggtcggagt catatattca aataaaattt ttttaagata
     4201 ggctttggat gagatataat agtggtgatt gatgtgttga tattatagtt gattgttaga
     4261 gaggatatta tagatgaata ttaatattga tattctaggt aattgctaga actaatatta
     4321 tagatgaata ttagtattga tattctaggt aattgctaga gttagtatta tagatgaata
     4381 ttagtattga tattctaggt aactgctaga gctaatatta tagatgaata ttagtattga
     4441 tattctaggt aattgctaga gctgatatta tagatgaata ttagtattga tattctaggt
     4501 aattgctaga gctgatatta taatttatta ttaatattaa tattctaggg aattatcact
     4561 atattttaaa aagtaaaaat atatatcaaa atgaactaaa taagtactgt aagaagtttg
     4621 ttaaattaag gtttgaataa agtgagggtg gagaacttat gatagaaata aaaactatag
     4681 aaataaataa aaaaagtcaa attgaccttt taatgttagc cgacccatgt gaagaaatga
     4741 ttgataaata tctcaataaa ggaactatgt acgcattgta tgataataaa gaacttactt
     4801 gtatagcagt tgtaaatgaa atttccaaag aaatatgtga attaaaaaac attgctacat
     4861 atgagcattt tcaaaatatg ggttatgcct caaagatgat ttatcatctt ttagatgttt
     4921 atagtaaaaa atattcatca atggttgttg gtacatctga aagcggagtt cctttttatg
     4981 agaagtttgg atttgtttat tcacataaaa ttaaaaactt ttttgtagat aattatccag
     5041 aaccaatctt tgaaggagaa ttacaatgtg ttgacatgtt gtatctatca tataactttt
     5101 agataggata atatttttag gtagatataa ttaattaatt catataatta tcggaattat
     5161 aatttataaa ttatgtataa taagagtata atttatttag aagctgttat attgtattat
     5221 attcaaataa gatatataat aatttgttta ttttgaattt tggaggatta attatggatt
     5281 caaataataa tactataaaa tcaactgtta aaaagggtat ttcttttggt tcttgtttag
     5341 caatgattat ttcttatact gcatggaaat ctattccatg ggctattttt catggcttaa
     5401 tgagttggat atatgtactt tattattggg ttaagtatgc atagattaaa ttaataaata
     5461 agtatgtaat attgtaaagt ttaagaaaca aaatgtaagg gagtgtctcg aagtgaactt
     5521 tttagtttat aaggcactct tttttgtatt aaataaaata tattttcatt acttaaacaa
     5581 tgaaaaagat gagtatctct attttaatac atacccttat attcattcat ttatcaacaa
     5641 tagttttaaa aagaaagcta tctaaaatct attgtaatct aagtgcttct ctttaaagtc
     5701 ttaactttag taatgatata taagtttata aggttcttat ttcaataatg caaattttac
     5761 ttagaagttc agactttggt atgaagaaat gggtaataat aaaaaagagg tgttgatatg
     5821 aaagaaaaat tagaagaaaa atctaagatt attaaaacat caaaagagta taaacgaact
     5881 ataattgaag aaaaggacag agaatgaacc aaaaaattaa atgacatact tagtagaggt
     5941 gatttttcta atgaaaaatt agataaaatc aaggatttaa ttccaaaaga aatattgact
     6001 agtgaaaatg ttggagaagt tgtaactgaa gatgggtatg ctaagcctga gtatgatgag
     6061 gtatttaaat cattgtttac tttaaataat gattacgatt tacttgcaaa ttttataaat
     6121 gatatcctca aagatgctcc atatgccaat aggaatatca aaccgttcac tcatataaaa
     6181 agaataatta aggctgaaac cgacccaaca attaattaca ttggtgagaa aagacctaga
     6241 ctttatattt tggctgaaga tgaagaaagt aatcatataa atattgagat gcaaagagct
     6301 cttgaagatg attatttgga aagagctgaa tattatctta gtcgtgtaca tggtagaaag
     6361 ttagaagaag gtaaagagta taaagaaatt ggaaaaactg ttggaataca tatactaaat
     6421 catgtaaaat ataatcacat agaagattat gtaaattgcc tgagattaac aatggacggg
     6481 catccaaata tttattctag taaaactgca ttatatttta tagaacttcc aaaaattcgt
     6541 aaatccagtt gtatagcaaa cagagttcta atatggggta aacttattga taatccttct
     6601 catattgata ttagaatact atctaaaaca gattatgtta ttaaaagagc acttgataga
     6661 ttaaaagaat taggcagtaa tgaggactat ttattaaatt taaagagagg tgcgtatatt
     6721 atgaataaaa gtagaaattt taaagaagaa atttttcaag aaggtgttga aaaagggaaa
     6781 agagaagaga aatttaatat catcataaaa ttaaaaaata aaggatataa tttgagtgaa
     6841 atatgtgata ttatagatga tttgaataaa tcagaggttg aaaaaatcta taatcaaaac
     6901 taatactata attgagatgg cactagataa atataaagct tcc
//
LOCUS       DAEPOZ010000192         6917 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.192, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000192 DAEPOZ010000000
VERSION     DAEPOZ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6917
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.192"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            241..2286
                     /locus_tag="KR440_002943"
     CDS             241..2286
                     /locus_tag="KR440_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0699953.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNILIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILNSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            2547..3746
                     /locus_tag="KR440_002944"
     CDS             2547..3746
                     /locus_tag="KR440_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HBH0699954.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDICRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARNLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            3798..4493
                     /locus_tag="KR440_002945"
     CDS             3798..4493
                     /locus_tag="KR440_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBH0699955.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            4506..5183
                     /locus_tag="KR440_002946"
     CDS             4506..5183
                     /locus_tag="KR440_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699956.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            5294..6895
                     /locus_tag="KR440_002947"
     CDS             5294..6895
                     /locus_tag="KR440_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBH0699957.1"
                     /translation="MIYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCCGKVITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     HDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGIDGVMDDLRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDNKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGIIKEGLIADIAVIDMDNYSYSRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
BASE COUNT         2770 a          683 c         1301 g         2163 t
ORIGIN      
        1 ttaaaaatgt attaatatat taacgaattt atattaaatt tttgtttata ttccaataat
       61 gtcaaactaa ccgttttaga aattaaatta tatgtgaaaa aagatagctt acattatttt
      121 attatggaaa ataatagtaa atggtacaaa atttgatttt taagatttaa aactttttta
      181 aaaagtgcta caatagacca aattattact ttctatactc gttaaacagg aggtgggact
      241 atgaaaaaac ataatatttt atttgtatca actgatgaca aaataaatat agatatatca
      301 aaacaattgg aaaatatatt tggagaattt tgtaatatag ataacttggt atatgtaaat
      361 aggataaata ttgaactgtc aagctatgag cttgtagtat gttcagacaa tgatataaag
      421 gaatatatac ataataatat tgataaaaat atacttattg taatagtaca tcgtactata
      481 aatattgaaa atataaatca gataataagt atagaaaatg attctgatgt tatggttata
      541 gatgcttata aagagtcagc agatgaaact gccaagataa ttagaaaact aggattaatt
      601 catattaatc taataccata ttatccaggt tgtgataagt ctaaatgtga gattggaatt
      661 attacgggaa gcagaaacag cataccacaa aacataaagc aaataattga cataggtgat
      721 aaggtaattg atataaatac agttatagaa atctttacaa aactaaatat atctatagat
      781 aaattacata ttattaagga aaaatacaat gaagatacag taagtggata taggtattat
      841 acaactatga ataaaaccat gaagagtttt ttggagatta tagatgaagg tattgcctct
      901 gttgataaat taggaaaatt tatatattgc aacaaagtat tttctaatct agttggaata
      961 gaccaaaatg aaattatatc taataacttt atggacttat ttagtgataa ggttgtcaaa
     1021 aaaatatttt ttcaagaaga tgaagttaat gatgaagtag taaatttaaa taacaaaaaa
     1081 ctgataatta ataaagtaaa tgtttatgaa aataatgaac gaataaagag tattataagc
     1141 attaaaaata taagtgcaat tcaagtgctt gaggacaaga ttcaaaataa acttcagtca
     1201 aaaggttttg tatcaaagta tacatttgag agtgttgtag gtgagagtaa aataataaaa
     1261 gaaaaaatta atattgcaag aaaaatagca actacagact tttctgtgtt gatacttgga
     1321 gaaaatggaa caggaaaaga aatatttgcc caggcaatac ataatgaatc tctaagaaaa
     1381 aacaagccat ttgttgcagt taatctatct tctttgtcag atacacttat tgaaagtgaa
     1441 ctttttggct atgatgaagg aagttttaca ggagcaataa aaggtggaaa aatgggtata
     1501 tttgaaagag cgcatacagg aacaattttt ttagatgaga taggtgatat atctttagat
     1561 gtacagcaga gattgctaag agtattacag gaaaaagaag ttatgagaat gggcggaagt
     1621 aaaattatac caatagatgt tagaattata gctgcaacaa ataaagattt aaagaaaaaa
     1681 attaaagaag gttcttttag agaagatttg tattatagga ttaatgtatt acatatagag
     1741 atacctagac ttagagaacg taaggaagat atacctctta taagtaagta ttttttagat
     1801 gaaataaata gcaacaaatg ttttacagag gaatcaatgc aagctttaac attgtatgaa
     1861 tggccaggaa atgtaagaga gcttaaaaat ttaatttatt atattgatac tatagttgag
     1921 gaagatagag tagattacga gcatttgcca gaacaattta gatttgaaaa aaacaatact
     1981 ttagttaatg aaaatttcga ttcaattatc ttagatttca aacaatctaa tttctttgag
     2041 gaaagtatat gtatattgaa cagtatagaa acttggaata ataaaaatat attattagga
     2101 agaaataaac tgcaagagat tttaaaagaa aaagatatag ttttaagtgt agaccaaatt
     2161 agaaaaagaa tagataagtt aaagagccat ggtttacttt tatctggagt aaaaaaacaa
     2221 ggtagtttta taactgatga aggaaagaac tttatatcat atgtaaaaat taagggagtg
     2281 atatagggga ctaaaaaggc aaaacaccct aaaaaaatcc ctataatgga tagaaataaa
     2341 taaaattatc tttaagatta agataattca aaaatattga ataaataatc taagaagatt
     2401 atttttaata aaacagtagt aaatatcaac cttactactg tttttatttt ttgcaagatt
     2461 tattatacaa ttaaaattta tattttggca tatcaattgc tacataggaa aaatataaaa
     2521 aattgtagaa cttggaggat gttgttatga agtcagctta caaaggatat gaagcctata
     2581 gttatttaga aaaaggaaaa gatatttcat ctattgagat gtgtaaagga gataaaagag
     2641 ttaaagagta ccttattgaa ttagatgata ttcaagaagc taaggttaaa agtatagtgg
     2701 atgaaaaaat atttatatca ttacatgaac atccagtatt atttccagaa cacctagaaa
     2761 gagatatatt tgaatataat agggaaggta aacaaagatg cgcttatgaa gcattatcaa
     2821 gaggatatta tgatgctata tttgataatt taatggatgg tgtatgtacg ataacatcaa
     2881 attcagggtg gaaatgggat gatatattaa tagatttagg catgagacta tgtgatttag
     2941 ctcatcaaga ttttgtcata aagtgtgaaa aagtagaaga tatatgcaga gctaaaagtg
     3001 aaggtaaagt agcattgata ccaacattag aaggtgcagc accaatagaa aatgaacttg
     3061 atagaataga tatactgtat ggatttggta taaggttaat gggtataaca tacagcgaat
     3121 ccaatgcatt aggttcagga ctaaaagaag agaaagatgg aggtcttaca aatttcggaa
     3181 aaaaagctgt tgaaagaatg aataaaattg gaatggcaat agattgctct cacgttggag
     3241 ttcaaactac acttgatgta atagaatata gcaaggaccc aataatatta agccatgttg
     3301 gggcaagaaa tttatggaac acaaagagac taactccaga tgaagtatta atagcatgtg
     3361 ctaaaaaagg aggggttata ggtgtagaag cagcacctca tactacaata acagagaaga
     3421 ataaagaaca ttcaatagag tcatttatgg aacattttga gtatattaaa aatttagtag
     3481 gaatagacca tgtagctttt ggaccagata cagtatatgg cgaccatgta aaattacact
     3541 cagtactagc tgctaaattt gatataaaaa aaataacagg tacaaaagaa catcccaaag
     3601 tagaatatgt aaaaggtctt gagaacccaa cagaaacttc aaaaaacatt gtcagatatt
     3661 tagtaaagca tggatatagt gatgaggaaa tagagaaggt actaggtgga aatatactta
     3721 gagtactaaa acaggtttgg aaatagatat attagaaata ttaataaaat ttgaggattt
     3781 atagggaggt tttataaatg gattatttaa aattagctaa tcatccactt ttgtggatag
     3841 cgagtacagt agcagtatca atagtagtgg tacaatccct tatatttgct ataaaatctt
     3901 gcaaagttgg taaaacaatg ggaataacag ataaacaaat aaagtcagct gttaagagta
     3961 gtgctatttc agctattggg ccttcaatga ctatatttgc tggaatggta tcgcttatag
     4021 taactatggg tgggcctata gcatggatga gattatcatt tataggttct gtaatttttg
     4081 aatcaatgtc agctggattt ggaacagatg cattaggtat aactttaggt tctcctgaaa
     4141 tgacaaaact tgcatttaca aatgcagttt ggactatgat attaggttct ttaggatgga
     4201 taatattcac attactattt ggacataaac tagataagat aaccaatgtt atatctagtg
     4261 gcaagaaatc atttatacca ataatatctc taggagcaat gttgggttca tttgcatatc
     4321 taaatgctga tagggtgttg cgttttgata atggaacaat tgcatgtata agtggaatgg
     4381 ctataatggt aatattgtgt atgcttgaaa agaagaaaaa tataaaatgg ttaagagagt
     4441 gggggcttac aatatcaatg tttagcggaa tgataatagc ttcagtaata taagggggga
     4501 gttttatgag taaagtaaaa tctatataca atgaagaata tttacctttt atgataaggt
     4561 atggaagact aacactatct ttaggtatta tagcagcatt agttcctgga ataattttga
     4621 gctttggatt tggtattatg cctccaatat cagcattatt agcaagtaca atggcaattg
     4681 tcagtatgag tgctccaaat tacataatag aaccagtttc ttattcacca atcttaggta
     4741 tcccaggaac ttatatgagt tttctttctg gtaatatatc taatatgagg ctaccatgtt
     4801 caatagcagc tcaaaaggca gcagaagttg aatcaggtac agaagaaggc tcgataatat
     4861 ctacaatagg aatagctgta tctattttag tgaatataag tatactaact ataggggtaa
     4921 ttttaggagg aagcgtatta tctaaaattc cagctgaagt agtagaaaaa ttaaacttaa
     4981 ttttaccagc tctatttggg tcagtttttg gacaagtatt tttacaagat aaaaaattag
     5041 gtctagtagc aattgtaata tctgtattga ctattatatt atcaaagcaa ggtataatac
     5101 cacagtctct agtagttctt atatgtgtat ttggtactat attaattgca agagctatgt
     5161 ataaagataa gcttagtgac taattacaat gaaataatag tgaattttca aatatgaaat
     5221 ataataattt gattttcaaa tataaaaatt acattaagat tatttaaaaa ttggttttta
     5281 aggagggaat attttgatat atgatttaat cttaaaaaat ggatttataa tagatggtac
     5341 aggtaatcct ggtttttatg gagatatagc aattaaagac aacttaatag caaaaataga
     5401 ttctaagata aatagcaata caaataaaga gattgattgt tgtggaaaag ttataacacc
     5461 tggatttatt gaccctcatg ttcatgaaga aatagtagca atactagatg gtaaatttga
     5521 aaagtttcta aaacaaggag ttacaacaac tataaatggt aattgtggac attctataac
     5581 accatattct tctgaaaatg tatatgaata tatgtataaa aatggtctat tacttgaaga
     5641 agaaaaaaag tatcttatag ataaaaataa atgttggaat aattttacag aatattgtga
     5701 ccttatatct aaaagtggta tttctgtcaa tatgggattc ttgttggggc atgggactat
     5761 aagatggagt gttatgggag gttctaagga tagacctcct acagaaaagg aaaaaaatga
     5821 aattacagat ataataaatg atggaatgaa aagtggtgct tttggaatat ctactggatt
     5881 ggcatatata ccaagtaagt atgctgatat tgatgaactt gttgatattg caaggcagat
     5941 taaagagcat gatggtatat atacatctca tattagagat tacataggaa gatataatgc
     6001 agtaaaagaa gccatagagg ttgggcaaaa aagtggtgct agggttcaag tatctcactt
     6061 aagtccagtt gaaatagaag catttgatga gatattaaag gctaggtata atggtgttga
     6121 aataatggtt gacacagtac caagaagttc tgggcattgt atgaagaaaa aaagagtaat
     6181 tcaatttatt atggcaatat catctagtct atttgaatta ggtatagatg gggtaatgga
     6241 tgatcttaga aatgaagagg gaagaacact tatattaaaa gaagcattta tacttggaga
     6301 tagggggagt ataatacttc taaacactaa agatattaat atggaaaaga aatcaatacg
     6361 tgaaatagct acacaaaaag gaatagatga ggacaagtta ttactcgatt tacttttaga
     6421 tggagacgaa gaacttatct tttgtttagg aggaatgtat agagctgatt tcccagataa
     6481 actacatgat aataaaataa ttgataatcc atttgttatg gttggttcag attgtttgtt
     6541 tagtgtgggt ggggatatgt catggtttga actacaaaga agaggagctt ttccaatttt
     6601 cttcaatatg tacagaaagt ctggtgttag attggaagag attgtaagaa gagttacttc
     6661 attaccagct aggcaattta aaataaaaaa tagaggtatt attaaagaag ggttgatagc
     6721 tgatatagca gttattgaca tggataacta tagttattct aggtcagaag atattgattt
     6781 ttcaaagcca caattactag ccaatggtgt tgaatatgta attgtaaatg gaaaaattgc
     6841 gttagaaaat ggagagatta ctgagaataa atgtggtgaa gtattaaaaa gataaggttt
     6901 taaaaataat aggattt
//
LOCUS       DAEPOZ010000193         6902 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.193, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000193 DAEPOZ010000000
VERSION     DAEPOZ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6902
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.193"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            124..507
                     /locus_tag="KR440_002948"
     CDS             124..507
                     /locus_tag="KR440_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699958.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKVVLKSPEYIGSHPKKPGKSVEFIKKIDNYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNNGRVKQYDG"
     gene            1013..1396
                     /locus_tag="KR440_002949"
     CDS             1013..1396
                     /locus_tag="KR440_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBH0699959.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            1398..1748
                     /locus_tag="KR440_002950"
     CDS             1398..1748
                     /locus_tag="KR440_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699960.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(1842..2300)
                     /locus_tag="KR440_002951"
     CDS             complement(1842..2300)
                     /locus_tag="KR440_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0699961.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLNEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(2345..3133)
                     /locus_tag="KR440_002952"
     CDS             complement(2345..3133)
                     /locus_tag="KR440_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH0699962.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(3272..3709)
                     /locus_tag="KR440_002953"
     CDS             complement(3272..3709)
                     /locus_tag="KR440_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HBH0699963.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKDCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            4531..5040
                     /locus_tag="KR440_002954"
     CDS             4531..5040
                     /locus_tag="KR440_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0699964.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDSLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVIVIVFVVLGGLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFLTRKKI"
     gene            complement(5951..6751)
                     /locus_tag="KR440_002955"
     CDS             complement(5951..6751)
                     /locus_tag="KR440_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0699965.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
BASE COUNT         2539 a          994 c          909 g         2460 t
ORIGIN      
        1 tgaaaaaact atattatact atcttttctt tttggaataa actatacttt aaatatagat
       61 atttaattta aataaatttt acagtattaa tagtaaatta tcttagaaag gaatgtgctt
      121 tatatgtcta cagacaaagt tgttggaatc atagatgaag aaacggcaga acttgcagga
      181 attgaatata ctggtaaaat ttatgcttct tctggtgtta taaaacatat aaagaaaaaa
      241 catagatgtc aactgagtaa ggatattttt aatgatataa tagacactat aaaagtggta
      301 ttaaagtcac ctgaatatat aggaagtcat cctaaaaaac ctggaaaaag tgtggagttt
      361 ataaaaaaaa ttgataatta tattttagta gctacagaat tagatacaaa aaaaggatat
      421 ttatatgtat cttcaatgta tgctataaaa gaagcaaaat tagaaagtag agtaaacaat
      481 ggaagagtaa aacaatatga tggttaacat aatattatta attttcccca aaatattgaa
      541 aaatattatt atataagtta taatattaat atacacgtag atatttatta gttagatttg
      601 aggttggaaa aggcacccaa tacgctacta tagagtagtt aatagagatg caggatacgc
      661 cgccctgcca aatctaatta aattaaaaag agaggattaa ttcctctctt tttgtttttt
      721 tgagatacta atttaatcta tatagaaaga ttgaaggtca tttatactaa aagttttcag
      781 ttatatacat taaactaatt aaataagata tttataatct atatatgata catattatat
      841 attaataaaa gaaaaatata tagaaaatcc ttataaaaat ttaaattgta atgaatagta
      901 agtaattaaa aagaattttt attgattttt aaaaatttag ttagaaacta tatcattatg
      961 gtaaagaatg tattataatg tttagaaata ttataataag gagcgattaa acatgaatct
     1021 atcgaagaaa atagaagata aaagaaaagc taaacagaga gctgaaaaag ttaagaaagc
     1081 taaaattgct acagctggtg tggtattagg agctgttact ggtgcagtga gtggagtact
     1141 tttggctcct aaatctggaa aagaaacaag agaggatata aaagatgctt cacaacaaat
     1201 tgctgaaaag ataaatatga aaactgtaga tgtaaaaggc aaagtatctg agaagctaga
     1261 ggataaaaaa ggaaacttta tagaatcaaa gaaaaaaata aaaaaatata tagataacaa
     1321 aaagagtgtt gaaaattcta gtgaggaaga taaaataata gatatagttg aaccagttag
     1381 tgttactgag gaataatatg gacaatacaa taacgattaa cataagtata atatatgtat
     1441 tattagctat acttgcttgt attgctttag tttatttaat tattgtactt gttaaactac
     1501 ataagatttt aaataatgtt ggaactatgt taaatgaaaa tactcaaaat ataaatacaa
     1561 ctttaaataa tcttcctaat gttgtagaaa cttttggaga agtaggagaa aatatgaaag
     1621 atgtaacaga agtagttact gaagttacag cagattttct tgttactaaa gacagtgtca
     1681 aatcaaatat ggatgttata gtcgatatat tgactataat taaaaatatt tttctagata
     1741 aaaagtaata aatttaatat aaaataagat ataatttaaa cgaccttaaa ataacattta
     1801 taaggtcgtt ttttattagc ttgatttgac actatgtgtt tttaataatt ggtaatattc
     1861 ctctttatca tttgaacata tatatctatg cttgttgttt caattacatc cataagaaca
     1921 tctttacatt caagctcaaa atcttccatt ccttctaaat attcatcatc ttcatcaaaa
     1981 aagatataag aattatatag ccattcttca aatttttctt caagtatatc ccaatacgaa
     2041 ctccaaaata atccacaacc tgatatataa gatgctctac tttgatttat ataaacctca
     2101 tttagaagct tttgtaatct actgctataa agaaaatctg ctcttccact gcttgatatt
     2161 aattcattta aatcttctat tgacattaat tcaactttac cacttttatc ataaggagta
     2221 taattttcat caacccattt atgtggtttg ttttctctgc atactgttgc tatcaactct
     2281 acaacttttg ttcttatcat ttttttatcc taacttattt attttttata aaataatcat
     2341 gattttataa tttatctatt tcttgtaatg ctaatttaac agtttcatat ctatcagcac
     2401 catttattac tacatcacct tttacaagtt tattagcttc tccacctaca acataaacct
     2461 ttttacctaa accttgctta taactagacg cctcaattat taaagaatct ttcaattgcc
     2521 aatgtaaaat ctgagcagcc actttatcag catcacctgt ataaacgatt acattttcat
     2581 acactttatt ttcccctccc ccttgagaat ttgatataga tttgtttagt ataccttctg
     2641 ctatcaattt agcaacttta tctttattat taacataata atctgtatct gctttactat
     2701 ctacaaaaca tacttctatt aatattgctg gtgcaatagt atgattaagc caataaagac
     2761 ctctaacatc agactttgca cccctatctt tgaatactgt agctaaccta gtattcactc
     2821 tttctgcata ggttttgcca ttattagtct tatatattgt ttctgtccct actggagttg
     2881 aagttgtagc atttgaatta aaatgaatct gaacagccaa atcagtttcc tgtttatttg
     2941 ctatagcaca ttggtctgct aaatgattac ttgattcatc aactcttcct gtataaacag
     3001 tagctccacc tattttaagc catttaacaa ttaagtcatt cagtattctt gtttctttgc
     3061 tttcgtttat atacccaaca gctcctgtac cttttccaat taaagtgtgc cctggtatta
     3121 ttactacttt caaactaaca tctcctttac aattaaattt aacttctatt taatatatgc
     3181 ataacctaga attttgtttc tttttatatc ttatactttt attataaccc attttgtaaa
     3241 aatatagaat attaagtaaa taatgttaaa attataattt tgtatataag acattacttg
     3301 attgcaataa ttttatagca tattcatcta ttctatatcc agttctataa tatatatgtt
     3361 taattccagc ttgaattata gccttagtac aatttaagca aggaaagtga gttacataaa
     3421 tagagcaatc tttaactgaa ataccttctt ttgcacaata ataaagggca ttttgttcag
     3481 cgtgaatggt tctaatacaa tgtccatctc tcatttcatg accaacttca ctacaatgct
     3541 tatctccaga aatactacca ttatatccag tagacactat tctgttttct gaattgacaa
     3601 ttatagctcc tacataagct ctatcacaag ttcctctttc agctactgtt tcgcacagtc
     3661 tcataaaata ttcttgccat gagcatctat tttcaagttt tttattcaaa tcatatacct
     3721 ccaaaattta tatctaatat ataagattta taccttaaaa ataatattaa aaacaaattt
     3781 atacgataaa taaaatttat taatactgtt tttagtctaa attatttcat tttaaattaa
     3841 agttacaagg aatctaaaat tatatatatc tttcttcgta atatgaatgt tacaatataa
     3901 aaaactgtct caataaaata gagacagttt tacagtcaaa gtttataata caagttcata
     3961 tttttcttta aattctttta cagcttcggg ccatgccttt tgttctgtaa tacctttttc
     4021 tttagctatt ttaattgcaa tctctcttat cattgcagcg ccttctaata ttcccatttt
     4081 taatctccct tcaaatatct ttttgtatag aatataaata gtgctaagat ttattacatg
     4141 ctgcatatac aatatctata caacacataa cttgtaaatt ccttttattt tcatacgtct
     4201 ttaaccgaca tataattata ttcaataagt tttaaaatta tgaactcaat tataattatt
     4261 tttatttttt tagaatctaa acactaaaaa ttgtaggtaa atttgtctta tgcctttata
     4321 aatatttcta cagttttaat tactggacat atctattttt attataaaaa gcgcttaaaa
     4381 atcgagaata tccgcgtaaa agctgtatat aagcaaaacg attaaaggaa aaagaagaaa
     4441 tttacttgaa tgttaaaatg cgttgcttga agcaacgggc agttttgcct ataatatcgg
     4501 taacaactga aagaaaggtg gttagcccta atgttaagcg aaaacattaa aacaatcagg
     4561 aagtcaaaag gactttcaca acaagaactt gctatcaaac tgaatgtagt gcgacaaaca
     4621 atatctaaat gggaacaagg actgtcggtt cccgattctg atatgttgat ttccatatca
     4681 gaggtgcttg aaacgcctgt aagcacttta cttggagaaa ctattgttga gtcggaagtt
     4741 gatagcttaa aagtgatttc tgaaaaatta gagattataa acttgcaact tgcacaaaga
     4801 aaaaacatga gaagaaaaat gtttcattgg ttatttattt tattatgtgc agtcatagta
     4861 atagtttttg tggtcttagg gggactaaat agtccttact tggattggaa ttataccgac
     4921 cctgaaaccg ctgtttttgg agtagctttt catgcttttg agtggctatt tgtcagatta
     4981 gtaccgattg tccttatagg agcaattatt ggaatctttt tgacacgtaa gaaaatatga
     5041 agtaactctg ctttttataa tatagcaagc gttccttaaa agtgtcattc tacatggcaa
     5101 tcgaggacaa ctatgtaagg aagaaccttt ttgatttcca actaagcgaa gttattgagg
     5161 acgccagtag gcccaaaacc gcattaagta aagtacagga agcaagactg ctggacttat
     5221 actctcatgc gtcagatgca gaaaatgcga ggtctgtagc atgatttaca acgatttgta
     5281 caacgactac gagtagaaat atatggagat aagtattatc tacgaaatca gaaaagaaat
     5341 gacttgataa tttactcatg ttataattat tgatgtgaaa ttaataaatc gaaattcctt
     5401 ttttagattt aacaatactg agtaaggaaa aagtgaatga cttttgcaag gaggtgttaa
     5461 tatcaggcaa ataaagtctt ttgtttttcc agttatatcc ttaatattct tagatcaaat
     5521 tagcaaagtt cttataggat tattcttaat ggactttgaa attgatataa ttgggaaatt
     5581 tttaagattc aatcctgttc aaaatacaaa attaagtctc tgctatctaa caaacggagc
     5641 acaaaaatga aaattaaata ttaaatataa agcacagtta attttgatta aaaagttgac
     5701 tgtcttttct tttttataaa tttaagcatg ggtaataaag aaatgactgc atttaagcat
     5761 tatgtatata attggaggca ataaaaagcc agcaaagccc ataaaataga gctgtgctag
     5821 caacatgatg agttataaga agataatcaa aagcttacgc tttattttaa tttaaaaaaa
     5881 tgattactgt aaagaaatca ttttttatta taatcactga tacatcaaat attctaaata
     5941 aaatttttat ttatctggaa ttgtatattt tttctataat attaactaca gcgtacattg
     6001 cccaagcaca aatagccaat acaaatactc ccatcattac taaatctaat ttaaatactt
     6061 gagaaccata tactatcaag tagcctattc catatcttga aactaaaaat tctccaacta
     6121 ttacacctac ccatgccata ccaatattaa ttttagttaa atttattaaa tttcctgtgt
     6181 ttgcaggtaa tatgagttta aataatattt gagatttagt agctccaaag ctttttaaca
     6241 tcttaatttt ttcttcatct atattcataa aataattata agctgaaagt atagtaacaa
     6301 caacagaaat cgttacagct gttacgacga ttccttctat tccagcacct acccaaacta
     6361 taattatagg agctaaagca gtcttaggaa gagcatttaa aactaccaaa aatgggtcta
     6421 atatttttga taatttttct gaccaccaaa gagcaattgc taccaaaata cctaatacag
     6481 tacctataac aagaccaaga acagtttcat atacagatat tccaatatgt ttaaataatt
     6541 gaccactaaa tgcatattga ataaataaat tgtaaatatc acttggtttg ctaaatagaa
     6601 atgtatttat aacatttaag tcagctaaaa gttcccagat tacaataaaa ccaattaaaa
     6661 ttaatagctg gtagaataat acatttcttt tttctctttt tacaccttta acatagttta
     6721 tatatccttc agatctttta ttaagcctca tgtgtatcta gctccttcca taatatgtcg
     6781 aaatactttt gaaaatcagg aactttacgt gttttgagag gcgtttttgt tccatccatg
     6841 ttaagttcaa tatcgtaaat attttttatg gtagctggtc ttttagatag tattgcaacc
     6901 tt
//
LOCUS       DAEPOZ010000194         6897 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.194, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000194 DAEPOZ010000000
VERSION     DAEPOZ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6897
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.194"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            392..1225
                     /locus_tag="KR440_002956"
     CDS             392..1225
                     /locus_tag="KR440_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBH0699966.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDEMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            1318..2250
                     /locus_tag="KR440_002957"
     CDS             1318..2250
                     /locus_tag="KR440_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBH0699967.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KMNLNTSYKEKMDKAGVDKEYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            2302..2784
                     /locus_tag="KR440_002958"
     CDS             2302..2784
                     /locus_tag="KR440_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH0699968.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            2796..3665
                     /locus_tag="KR440_002959"
     CDS             2796..3665
                     /locus_tag="KR440_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBH0699969.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGVIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKAHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            3708..4463
                     /locus_tag="KR440_002960"
     CDS             3708..4463
                     /locus_tag="KR440_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBH0699970.1"
                     /translation="MTTYKKLKKSIFKISIFSICLLVSLVDINPIFAGSSSNETSNSD
                     TIKVLNAKSLNFKQGNYEVNVSIPKIKGLKDKTLENKLNNEFLQDGKKIYSEFQNKMK
                     KENNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEDSSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIRAISDNIKEQMRNQIKKDDTKSYFIDQDKDFPVKDFESINKYQDFYFNKN
                     GNLVISFEEYEVVPGYMGAVEFVIPNKVIKRLR"
     gene            4505..5326
                     /locus_tag="KR440_002961"
     CDS             4505..5326
                     /locus_tag="KR440_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HBH0699971.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANYER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKRETGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            5331..6092
                     /locus_tag="KR440_002962"
     CDS             5331..6092
                     /locus_tag="KR440_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBH0699972.1"
                     /translation="MNTGSKDIDTLNVNFRGIEIKAVLVYKNRKNISIKIDPFGKIIV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKYKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLKDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERTVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    6253..6510
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6623..6862
                     /locus_tag="KR440_002963"
     CDS             6623..6862
                     /locus_tag="KR440_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBH0699973.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
BASE COUNT         3048 a          635 c         1154 g         2060 t
ORIGIN      
        1 taaataagat tagaaaattt tatttgataa caaaaattag acaatatgaa tatataatgt
       61 atgtattaaa aatgtggcaa atagttgttt tggtatttat tatttgttta atatttctaa
      121 tttataaaaa aaaaattata aattttaaaa attataaatg acattttaac tagaattaat
      181 tgtaaaaaac actatataat actcaatttt caacattcta tacaattata cagaaattat
      241 acagcataat aatcaccgaa ataacaaatt ttaaaaatta agcaaaactt tttgtcgaag
      301 ttcaacgtct atatatatgt agtgtaaatt tttgggataa aatactacaa aaatataaat
      361 gtaaaaatat agtaatattg gaggtcgaac gatgagaaat aaaaagaaaa aaataaatag
      421 aaagaagttg tatttgcttt taagtgctat tgtagtgtgt ctggctttta tagtagttgg
      481 tgttcgcgtc agctctttaa atatgaagga aaatgaaatt agtagaaatg caaaactgtc
      541 aagttctact attacagata tagtatctaa tacatcaata gaagttagta aagggccaag
      601 taaaagttct ggaaaaatag catacataac tatagatgat ggcccatcta aatttactga
      661 cgagatgata aaaactttaa ataaatacaa tgttaaagca acttttttta tgatagatgg
      721 gaatatgaag gaatatccac agcaagttaa aaatataatt aaaaatggga acacagcagg
      781 ttttcatagt gtatcacatg atattcacaa gctttatgtt actagtacat cagcaaaaga
      841 ggaatttgat acaaatgatc aaacttttta taagataact ggtaaacatt caaaagtaat
      901 aagaatacct tatggaagca aaccatatac accacaagcc tcttatcagg ctttagttga
      961 tgctggatat aaaatttggg actgggactt agatacagaa gattggagat ctaattcttc
     1021 tcaaattgta caaaatgtaa aaaatcatat taagaataga aaaggtgaag ataaagacca
     1081 acttgtagtt ttaatgcatg agaaaaaaca gagtgcagaa gctttagatt cagttttaaa
     1141 attcttatca gatgaaggtt atgaatttgc gccagtagat caaaaccaaa taccaaaaaa
     1201 ttattggtta cgtaatttag agtagttaaa atataagaat attgattgat atttttagaa
     1261 attgagaata aaaatgagtg aggtgtaggt atattgaata aaaaacttta tataggaatg
     1321 gtcggaatat tgtctttgat gatggttggt tgtaataaaa gtttagcaaa agtaaatgat
     1381 gtggaaatca caaaagaaca gtacaaaaaa acaaaagcag ttttatctgc tactaataat
     1441 tatataaatg ggcagtcttt agatgaatta gaaaaaactt tggataaaaa aggtagaaat
     1501 aaattagaaa atgttataat atcgtttatg gtggataatg aacttttata tcaagaggct
     1561 aaggataaag gactaactcc aagtaaaagt gaagtagact caaaatatca agaactagaa
     1621 gataagatga atttgaatac aagctataag gaaaaaatgg acaaagcagg tgttgataaa
     1681 gaatatttaa aacaagaaat atctagagat ttagcaatag ataaaaataa aaaggccttt
     1741 gaagatagaa taaacataag tgataatgat atggaagcct actatacaag tcataaaaaa
     1801 gattttaatg tagaagaagt aagtgcttct cagatactta tatcgacttt agataaaaat
     1861 aaaaaagaag taagcaaaga taaaaaagaa gctttgaaga agaaggcaga taatatattg
     1921 actaaaatca aaaatggaga aagttttgaa agtttagcta aaaaatattc ggatgataaa
     1981 gcaactggaa aaaatggagg acagctagga tattttacaa aggatgataa aaatgctgag
     2041 tttacaaagg aagtgttcaa attaaagaaa aatgaagttt caaatgtgtt tgaaactagt
     2101 tatggatatc atatagtcaa agttactgat aaaagagaaa gacaaaaaag ttttaatgag
     2161 tgtcagagtt taataagaga atctatttta aatgaaaagt atattgagca tataaaaaaa
     2221 ctgaatgaag atgctaaaat agacagatag aatttctaac agctcaatcc tagcctgtaa
     2281 tcggagggag attttacagg aatgtttaag agtttaaaga gaacgaaagt tgagaaatac
     2341 attatcgata ataaagactc tttttataga atagcatata gttatacgaa gaatgaagaa
     2401 gatgccttag atgtggttca agaagccatg tataaggcac tatactcagt ggaaaatatt
     2461 aaagaagtaa attatataaa gacatggttt tataaaattt tagttagaac gagtatagat
     2521 tttattagaa aaaaccgtaa atataataat atgacagata ttgaccttat agatgagact
     2581 ggagagtatg ataaatatac tgatttggat ttaagaagag ctttggaaga attacctatt
     2641 gaatacaaat ctattataat attaagattt tttgaagatt taaagattga agaagtagca
     2701 ataatattgg atgaaaatgt gaatactgtt aaaactaggt tatatactgc tttaaaaaaa
     2761 ctaaaattaa aaattgaaga gtagggataa aaagaatgac aagcaaagaa aggttaaata
     2821 agttaaaaga tgagtatcat aaaattccaa tacccaagaa attagatact ataataaatc
     2881 atgaaaaaat agaaaacata tatcgagaga aaaataaaaa agtaaataga ttaagattta
     2941 aagttgcaat agcctttgca tgtatattta cagtattagt aaatataagt cctgtatttg
     3001 cagacaattt ttctaagata cctgtaattg gagtaatagt agaagtaata actataaaaa
     3061 attatagttt aaaaagtgaa aattatgaag ctgagattga tattcctaaa atacgaggtt
     3121 taaaagataa aaacttagaa caaagattaa acagtagttt tatggaagat ggaaaaaggc
     3181 tttatcatca attccaagag agaatggaga agatacaatc atcaaagaat aaaggatata
     3241 aatctttaag tttgagttat agtgtaaaaa ataattctaa aaaatttcta tcaatagaga
     3301 tgacaaaaaa tgagatagag gcttcatcat atgtgagtaa ggcacattat actattgata
     3361 aaaaaagaca aatagttttg actttgccaa tgctttttaa agatgataag tatataaaag
     3421 ttataagtga taatataaaa gaacaaatga gagaacaaat gaaaaaggat agtacaaaat
     3481 cctattttat agatcaaaaa aaagacttgc ctgttgaaga ttttaaaaca attaataagt
     3541 atcaggattt ttattttaat aaaaatgaag accttgtaat ttgttttgat gaatatgaag
     3601 tagcacctgg ttatatgggt gcagttgagt ttgtaatacc atataaagta ataaaagatt
     3661 tgtaaattga atagtagttt atatatacat attttaagaa ggacaatatg actacatata
     3721 aaaagcttaa aaaatctata tttaaaattt ctatattttc aatatgctta ttagtctcat
     3781 tagtagatat aaaccctata tttgcaggta gttcctctaa tgagacttca aatagtgata
     3841 cgataaaggt gttaaatgct aagagtctca actttaaaca aggcaattat gaagtgaatg
     3901 tgagtattcc taaaataaaa ggtttaaaag ataaaacttt agagaataaa ttaaacaatg
     3961 agtttttgca agatgggaaa aaaatttaca gtgaatttca aaataaaatg aaaaaggaaa
     4021 ataataaggg tcatagatac ctaaatgtaa tccataatgt caaaaataat tctaaggact
     4081 ttttatcaat agaaatgaca aaagaagaga tagaagattc ctcgtctata actaagaagc
     4141 attacactgt tgataaaaat agaggaatac tattaaattt accaatattt tttaaagata
     4201 gtagatatat tagagctata agtgataata taaaagagca gatgagaaat caaattaaga
     4261 aagatgatac aaaatcttat tttatagacc aagataaaga cttcccagtg aaagattttg
     4321 aatctataaa taagtatcaa gatttttatt ttaataaaaa tggaaatctt gtgattagtt
     4381 ttgaagaata tgaagtagta cctggttata tgggagcagt agaatttgtg ataccaaata
     4441 aagtaataaa aagactaaga tagatatata gaagattttt agatagactg gtaaggagaa
     4501 ttatatgaat atatatgaac aagcaataag attaatagaa aataatgaag atttcgcatt
     4561 tgctacaata acgtctcatt caggttcaac accacgtgaa actggagcaa tgatgattgt
     4621 gaaaaatgat tctactatat ttggttcagt aggaggaggt agtgtagaag cagaatgtat
     4681 aaaacatgca attaatgtta taaaaaatag agaatcaatg ttgtataaat ttactctaaa
     4741 caaatctgat gtggcaaaac taggaatgat atgtggaggt actggagaaa tacaaataga
     4801 ttttatagat agtaaattaa aaagtaatat agaaaaattc aataagagac taaaggaaaa
     4861 tacaagtaaa gcatatatat ttggtgcagg tcatatttca agagatgttg ctgtgatact
     4921 atctctttta gaatttagaa cagtagtaat agatgataga gaagaatttg ctaattatga
     4981 aaggttccca gaatcagaag tagtagtttt agattctttt gaaaatatac cagattttcc
     5041 aactgacgaa aatagctata taattatttt aacaagaggt catctatatg attcaagtgc
     5101 tttagaatgg gctcttaaaa gagaaactgg ttatataggt atgattggaa gtagaacaaa
     5161 aattggattg acgtacgaaa aactcatgaa aaaaggtttt aagaaggaag aattatcaaa
     5221 agtacatgca ccaataggca taaaattgaa tgctcaaaca ccagctgaaa tagctgtatg
     5281 tatagctgct gaacttataa attgtagagc taataaggaa aaataaacta atgaacacag
     5341 ggagtaaaga tattgatact ttaaatgtta attttagagg gattgaaata aaagcagttt
     5401 tagtatataa aaatagaaaa aatatttcta taaaaataga tccatttggc aaaataattg
     5461 taatgagtcc tcctaaaata tccaaaaaaa taataaaaga tataattatt gaaaaaggag
     5521 actggatact taaaaagtca gaaaaatata aaggaagaga agaagtatac aaacaaagaa
     5581 tgtttataac tggcgaaaaa tttctttatt taggtgaaga gtaccatcta gttataaaag
     5641 aactgttgaa agattctgtc aaaaaaagta actgtagaat aactattaac gaatatcaaa
     5701 tagttgttca gacaaatgat acaagtacag attttattaa aaaatcttta aaatcttggt
     5761 ataagatgga aagtgaaaga acagtattag agagaataga ttttcttaaa ttaaattgtg
     5821 aaataatgaa gcaattagtt ccagcaagtg ttaaggtaaa agaacaaaat aagagatggg
     5881 gaagttgtac agctcaaaaa aatatttata taaactcaaa aatatctatg gcaagattag
     5941 atgtaattga ttatgtaata atacatgagt ttagccattt agtacatatg aatcactcta
     6001 aaaaattcta tgatttagta aaatccatta tgcccgatta taaagataaa gaaaattggc
     6061 taaaaataaa tggatataaa ataataatct gaaaacgatt attccaaaat acaggcgtaa
     6121 agtataaaag aagtattatt gtgaaaaaaa taaataatct gaaaatttta aaaaaagtca
     6181 ttgacattgt aaatgatagg gaatataatc tatattaaca acaaaacaga attaaaatat
     6241 aaaattaaaa aattaaaagc aatgacagag acaacaatac taatagtgtt atccacagag
     6301 aaccaatgtt gctgagaatt ggtgttaaca gtagtataat gatcactctg gagcttttgt
     6361 actgaatatt tttagtaagt acaaacgtat gtcaacgtta catgactaag gttaaggtaa
     6421 ttaattaccc gacctgaaaa aagaggggta gatgtatttt ttatatctac aaataaggtg
     6481 gtaccgcgga atataacttt cgtccttata agtaatattt atattgctta taaagatgaa
     6541 agttttttta ttttaagcga tataaataaa tttaatccat ataaaattaa taaaaaaata
     6601 ataaaaataa ggagggattt ttatggaaaa tactgtatat gctaaattaa accaaggaat
     6661 agattctttt ttaagagtgg ctatgactct aagaagaaga gaggttgata tacaatcaat
     6721 aagtatgact gtagataata tgaataattc aggtataaaa ttaatagtaa atgaagaaaa
     6781 gacatcttta gattctgtct taaaccatat gaaaaaacta catgatataa gagagataac
     6841 tgttgaaaga atgagacatt aattaaatat attttataaa gctaaagata aatatta
//
LOCUS       DAEPOZ010000195         6777 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.195, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000195 DAEPOZ010000000
VERSION     DAEPOZ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6777
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.195"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            7..1185
                     /locus_tag="KR440_002964"
     CDS             7..1185
                     /locus_tag="KR440_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0699974.1"
                     /translation="MSNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIGIII
                     GAYGISQCLLRIPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIANILS
                     GISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMTALC
                     IMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQGIQM
                     STTMSFTTQILKNLGSSSTLIGLSSISYMVCAVASSGFASTKFCTKYGPKFWIPLVFI
                     LVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGFFQA
                     IYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDIIQS
                     "
     gene            1211..1903
                     /locus_tag="KR440_002965"
     CDS             1211..1903
                     /locus_tag="KR440_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBH0699975.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMMAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(2119..2421)
                     /locus_tag="KR440_002966"
     CDS             complement(2119..2421)
                     /locus_tag="KR440_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HBH0699976.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(2443..2853)
                     /locus_tag="KR440_002967"
     CDS             complement(2443..2853)
                     /locus_tag="KR440_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBH0699977.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            complement(3028..4956)
                     /locus_tag="KR440_002968"
     CDS             complement(3028..4956)
                     /locus_tag="KR440_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0699978.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFILSNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFENLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            complement(5132..5620)
                     /locus_tag="KR440_002969"
     CDS             complement(5132..5620)
                     /locus_tag="KR440_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH0699979.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEDGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            complement(5756..6766)
                     /locus_tag="KR440_002970"
     CDS             complement(5756..6766)
                     /locus_tag="KR440_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBH0699980.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEESTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
BASE COUNT         2274 a         1176 c          727 g         2600 t
ORIGIN      
        1 aaaaaaatat ctaatcaaaa attactttta ctttctattg ttacattttt ctggttttca
       61 caatatgtat atataccttt ccaaaatcca tatttaagtg catgtaatgt atctacaaat
      121 atgattggta taataatagg tgcatatgga atctcacaat gtttattaag gattcctgtt
      181 ggagtatttg ctgattctat aaataaacat aaaatattta tactgtctgg aattttcttt
      241 gctggtttag catctcttat aagagtcttt tctcctggag gcaatggttt tcttatagct
      301 aacattttat ctggaatatc atcatcaact tggatttctt acatggttca ctataccaac
      361 tttttttcta cagaacatca acaaaaagct acgagtacga taatattggt aaacaatcta
      421 ggaatgttga ttggatttgt tatgagtact ttattttatg aaaaattagg aatgacagcc
      481 ttatgtatca tgagtgtatt gtcttcagtt attggaacga ttctagcctt aaatataaaa
      541 aatcataatt caaaaaaaat acctgaaaaa atatcaatta aaaaatattt atctatctgc
      601 aaagataaaa gacttgtatt tttttccttt atggcattaa ttcaacaggg tattcaaatg
      661 tctacaacaa tgtcatttac tactcaaatt cttaaaaact taggttctag ttctaccctc
      721 ataggtctat cttctatatc ttacatggtg tgtgctgttg cttcttctgg ctttgcttct
      781 actaaatttt gtactaagta tgggccaaaa ttttggattc ctttagtttt tattttagtt
      841 tcagcctatt gctttttagt tccaacaata aaaaatatta atataatatt tgtactacaa
      901 gtgctaccag gtatatctac aggtatactc ctttcttacc ttactagcga gtctatgaaa
      961 aatgtaccaa aacatcagaa atctactgca atgggttttt ttcaagcaat ttatgctata
     1021 ggaatgtctg tatttccaat gatagttggt aaaatatcgg aatatacttc aatacaatat
     1081 ggatacttta tcttggctat tatagctatt ttggcctcta tcctgtctta tacatattat
     1141 aaaaaagtag atttaactct taactatgat ataatacaat cataaaaaaa ttatatgttt
     1201 aggagttttt atgaaaagct taaatgccac tgaaaaaatt attgattata ttaaattaaa
     1261 cattctaaat ggaactttta aaattaatag taaattacca agtgagagaa agattgctga
     1321 cctatttaac ataagtagaa tacctgtaag aaatgccata aatatactct gtaaggaagg
     1381 aatacttaga gctgtgcctt actcaagtcc tatagttgaa ggatttaaaa aagttgacct
     1441 atttgatgat ggtgaaatct ataaaaatca taacatacaa gaattttatg tagaatcttt
     1501 aagagcaaga caacttatcg aaagtgaagc tactaaacta gcaattttaa atgctacatc
     1561 taaagaactt caaaaaataa aatatactta cctaaagtca atagaggaat tggataaggt
     1621 ttctcatggt ttgatagaag aatgttatga tgctgactta caatttcaca aagagataat
     1681 tttagcttct cataatccaa tttttataaa atattatgag ttaataccta aaacagtttc
     1741 ttctaaccaa tacttcggtt ttaaatatag aaattctctt aaggatatga ttagccacca
     1801 taacaagata atgatggcat ttgatagtaa agactcttac ttaggttata catcaatgta
     1861 caatcattta gaagatgtaa ttcagttatt tcaacatgat taaacttcta ttttacctta
     1921 aatgcaacta acatataaaa atggagtgtc tcaaagtgaa ctttttagtt atgaggcact
     1981 cttttttgta tgaaatcaaa tatattttca tcatttcaac aatgaaaaag agtcttatct
     2041 atagtttgag acagcccttt atttcttatg taaaatatgt aaaaaggcgt cgctctattc
     2101 ttagtgcgac acccacgatt aatttaagtc tattaaaatt tcacttacta tagattttgc
     2161 atcctctatt atatactttg gatcaacttc ataaaattct ctatctataa atctatcaat
     2221 cttctctatg ctttctatag aatcacttgt aacaaataac actatagatg catcttctat
     2281 atcataaata gaaagttcat tttttatatt atcattttct tgaatttcat aaactaaatc
     2341 aaatcctaaa tcatttgcag ctgtcttaag agaatttaaa cttctttcac tttcacaaac
     2401 tgctactatc tttttattca tatttcaaat acctttctaa ttctactccg gtacaatcat
     2461 cactatacat tttgcttgtt taactacact tgctgttact gaacctatca ttcttgtaag
     2521 acctttttta gtagattttg tcataactat aaaatctaca ttatcttctt ttgcttttct
     2581 aattatctca tctcctggat atccaaaagt aaaatgtact ttaacatcat actctcccat
     2641 tatctctgct gctttatcca agattcttct tcctaaattt tcagaatttt ttatctcttc
     2701 agccagggat atgccatcaa taaatactag ttcttttaca ttcattattt ctacttcaac
     2761 ttcatctttt ttaaatattc cttttataaa ttccaaagaa tgcatacttc tttctgtccc
     2821 atcaattggc actaatattt tctttttatt catgtcacat cttcctttct cactttagga
     2881 taattaaata aaatataata aatctttact ccattaatta atattaataa tatgtataat
     2941 aaatttaata tttattattg tgttaatttt ataatatatc gctggcagtg tcaatgaaca
     3001 aagttgtaat taaaaaaata aagggtgtta acttgtaaaa gtattttctt tcaacaaatt
     3061 tttaaaaaca tcttctggaa ctggtttaga aaaataatac ccttgcaaat attcacactc
     3121 catagctatt aaatcttcag cacacttaat ggtttcaaca ccttctgcta cagccttcat
     3181 attcaattta tttataagag ctataatcgt ctcaactgta agcttatgcc tactatcttc
     3241 attcatagac caaatcaaac tcttatctaa cttaattact gaaaatggta aagaaagcat
     3301 attagtaact ccagaataag caattccaaa atcatcaagt gcaaacttaa cacctaaatc
     3361 aattaatctt tccattgttt ctttaattat attaaaagag cccatagcca cagtctcagt
     3421 aatttcaagt ttaaatttag ttggtgatac attatattta ttcataagat aaagtaaatc
     3481 atcagctaag tttttttgca tacattgtac tacagaaaga ttcacagcta tattatccaa
     3541 ttttaaattt tcaaactcat cactagctat aaatctacat accttctcaa agactactaa
     3601 tccaattgag ttaatcaatc ctgtttgttc tgcaattgga ataaattcat caggaggtat
     3661 aaaacctaac tctttgtgtt ctagcctaag taatgcctct gctgaagtaa acctgcccct
     3721 gctattagaa aatataggtt ggtagtacac ttcaaaccca tcatttttta atgcttcttt
     3781 taaacaattt ttaataacat gtttcttctt tatcttttca cttatagacg catctgcata
     3841 aacaactcgc cttgactcag aacgttttgc ttcatgtata gagaaatcta tagcaacaat
     3901 tacatcttct gtcgtttctg cattttctgg gtaataaaca acagcaatac tagttgctaa
     3961 attaaaactt atctcttcta ctagccaagg ctttttaaat ctttcttcta tctttctgat
     4021 aatatcatct ggttctatac ctaattgact attgacaaca atagaaaata tatctccccc
     4081 tattctatat acatggttta catcatgtat ctgaatcaaa tattttccaa cttcttttag
     4141 tgcaatatct ccattttttg ttccaaaagt ttcattcata aacttaaaat catcaagcgc
     4201 aattgtaata ataaatcctg attgttcagt attgatattc aaatctagct cttgaataaa
     4261 agattgtcta ttgagtacac cagtaaggtc atcaataata agacctcgat tctgcataga
     4321 aagatatgtc aaaagtaaac tacatgcaca tgcagttcct gtaagaagat actgtaaaaa
     4381 aattgcctgt aagataattg ccacaattga aactataaga aaacctctaa tagcccatat
     4441 ttttgattca tgcattgctt ttctatattt aaaagataaa aatgctgaat atattgcata
     4501 aaagaaaaaa gatgcaaaag ttgcaacata tccacaaccc cgtatataac ctgtttcttt
     4561 tgacaatcca aatagaatat gcgtaaaagg atttgtaata gataagatta tacaaattat
     4621 aagtggtata gataaaaata ataatctttt tttcatatac cttatatctt gataaataat
     4681 agccataaca taaaatgata caagagctgc tggtattggc aataacaata agtatatctg
     4741 attcaataaa aaagtaataa aaattggaat tttgtctata ttatttagac aaaatgaacc
     4801 taaaatattt gaaagtataa ataaaaatga gaatattaaa agtgcacaat atattttgtt
     4861 ttgtaaagta ggtataaatt tacttctaaa aaagtatacc attaaaaaaa taactataat
     4921 tgcagaaaaa atctcatatt ctatatgcca taccataaac ttccctcgct attgacgttt
     4981 gattttaaat attttaataa tataatttat ttataattat attataattc atcttgagta
     5041 attttatgga aaatttttta ttttataact tttacttcaa atctccatta aaaccctaaa
     5101 tttactctaa tcattattat gcatatagaa attaagcaaa tagttctttt gccttagtag
     5161 caattctttc accaaatatt atagccttct catcttgtaa ctctggacct tcaattgaat
     5221 gtacatgtcc taaatgtata aatggctttc ctaaagaacc acctccagaa tacacaagca
     5281 tccctttaac catcaaatgt cctactatag taagtatcgc aacatctgga cctccacata
     5341 tataatcaca tgtagcaaaa acagctccta attttccagc taaattaact ttttttgaat
     5401 cttcatcaaa ccattttttt acttcccaag ttgtatttgc taaataagta ggtgttccaa
     5461 atattactgc tttactttca tttaaataat ctaaatcaat attatcaagt ctcataagct
     5521 ttacatctac tccatccacc tttttagctc catcttctat cagcttagcg accctttctg
     5581 tgtttccact tcttgtttgg tataatactg taatcttcaa ctttaattcc cccttatcta
     5641 ataaaaataa tttattatac atcaaattat atcactaatt ttgctgtcct acaaagagct
     5701 agtcgattat ttttttatta actatttccc ataaaaaaca cctacattta ttactttaaa
     5761 aaacacctaa actcactttt aacttgctta tttcaatctc aactatatct gatatatctt
     5821 caaaagacat gcctctatca taaagttttc ctgcaatttt gatttttgca ttttcttctc
     5881 cttttttaat caatgctctg ttttcttttt ctattacatt tctaacatat ctctttgcaa
     5941 atggttcatt attatcatat agagccttgt atttttcctt atctcctttt acaaaactaa
     6001 gtgctctttt atcttgtttt atagcttctg tacaaatttc ttctgtttgt tcttttacaa
     6061 attttaatgc cattccatat tgtcttactg cctttaagca tataccaaca gtctgttcct
     6121 ttacaaattc taatgccatt ccattttgtc ttactgctct taaacagatt gtttctgttt
     6181 gattttttac aaatttcaaa tcaagcccat tttgtctcac tgcttccata catatttctt
     6241 ctgtttgatt ttctacccat tttagattat agctacaatg cttaactgct tccatacata
     6301 ttttttctgt ttgatttttc actcttgcca atccaaattc gctgtttcta actgcttcta
     6361 tacatatttc ttctgtttgt tcttttacat actccaaagc cgcaccattt ttttcaacgg
     6421 ctcttaagca taactcctct gtctgatttt ctatatattt taatgctgtt ccatcttggt
     6481 taatagccaa catgcaaatt tcttctgttg actcttccac aaattctaac attctccaat
     6541 cttgtttaac tgcttctata catatttttt ctgtctgatt ctgaacacat tttattgcat
     6601 tacaattttg tctaacagcc tctagacata tctcttctgt ttgatttttt atgtatatta
     6661 aattcaatcc attttttctt actagctcta aacattcttc ttctgtttta ttatcaaaat
     6721 cctcagtatc tattgattca aatatatcat ttttatcttc atgcatatct tagactc
//
LOCUS       DAEPOZ010000196         6705 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.196, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000196 DAEPOZ010000000
VERSION     DAEPOZ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6705
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.196"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..1494
                     /gene="cas3"
                     /locus_tag="KR440_002971"
     CDS             <1..1494
                     /gene="cas3"
                     /locus_tag="KR440_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HBH0699981.1"
                     /translation="GKTAADYRKTFKRKFFLVPNKISAESFYRKNIFQNKNDLDTRSS
                     NDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCVIATVDQLLLSMFKFP
                     GYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDVDFHLMTATLPKSYKE
                     QMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVSFIKEKEIKSIVEGAL
                     ENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRFKFKDKKEKYSKILNG
                     EGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNNKYEYGNFYILPSEDK
                     IYDNKLKNTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVKNYFEENFISCDKEIK
                     NIYGINKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSYKIILEEDFYKEDREL
                     QQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVGLILK"
     gene            1935..2882
                     /gene="prmA"
                     /locus_tag="KR440_002972"
     CDS             1935..2882
                     /gene="prmA"
                     /locus_tag="KR440_002972"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBH0699982.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQDGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            2901..3659
                     /locus_tag="KR440_002973"
     CDS             2901..3659
                     /locus_tag="KR440_002973"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBH0699983.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNNVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            3661..4959
                     /gene="mtaB"
                     /locus_tag="KR440_002974"
     CDS             3661..4959
                     /gene="mtaB"
                     /locus_tag="KR440_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HBH0699984.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            5002..5352
                     /locus_tag="KR440_002975"
     CDS             5002..5352
                     /locus_tag="KR440_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HBH0699985.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            5515..5694
                     /locus_tag="KR440_002976"
     CDS             5515..5694
                     /locus_tag="KR440_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBH0699986.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            5724..6167
                     /locus_tag="KR440_002977"
     CDS             5724..6167
                     /locus_tag="KR440_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBH0699987.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            6278..>6705
                     /locus_tag="KR440_002978"
     CDS             6278..>6705
                     /locus_tag="KR440_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBH0699988.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYI"
BASE COUNT         2780 a          629 c         1280 g         2016 t
ORIGIN      
        1 ggaaaaacag ctgcagatta tagaaagact tttaaaagaa aattcttttt agtgccaaat
       61 aaaatatcag cagaaagttt ttatagaaaa aatatttttc aaaataaaaa tgatttagat
      121 acaagaagta gtaatgatta cattggatta cttcatggag atataaactt atattcagag
      181 aatgaagata atggagaaca tgattttgta ttgacattaa gggatataga tttgagtata
      241 aatttttgta aaccttgtgt aattgcaaca gttgaccagt tattacttag tatgtttaaa
      301 tttccgggat atgaaaaaat attcgcagcc gtaaaaaatg catctataac tgttgatgaa
      361 gtacatttat tggaaccaaa gatgtttttg attttgatat attttatgca gtttgcatgc
      421 aagtacttag atgttgattt tcacttaatg acagctacac ttccaaaatc atataaagag
      481 caaatgataa acaagggaat tatttttcaa gaagaaagta atgaaaatgt tactgataaa
      541 ggagaaatag tttttattga gagcaacaag gaagaagaaa tttgtgaagg taaagatgta
      601 aaggtttctt ttataaagga aaaagaaatc aagtcaatag ttgaaggtgc tttagaaaat
      661 aaacaaaaaa ttcttataat aaagaatact attgattcag tcaatagaac atatgagttt
      721 ttaaaagaaa atttatctga taagtatggt ggtgtagata tagatgtttt acatagtaga
      781 tttaagttca aagataaaaa agagaaatat agtaaaatat taaatggaga gggtgatatt
      841 tggatatcaa ctcaatcagt agaaatatcc ttggatttgg atttcaatat aataataagt
      901 gatttagcaa ctatggatag cttaatacaa agaatgggta gatgcaatag aaataataaa
      961 tatgaatatg gaaactttta tattctacct agtgaagata aaatttatga taacaaactt
     1021 aagaacacaa ctaaaaacat cttaaaggac atattaaaaa ctaactctat ttttacaatg
     1081 gggattagaa aaactatttt ggatgattac tatgataata gtgtagttaa aaactatttt
     1141 gaagagaatt ttataagttg tgataaagaa attaagaata tttatggtat aaataaagaa
     1201 atttttgatg gattggattt aatttttaat tttgaacctt acaaaaatat tgttgatagt
     1261 aagaaggaag cagctaaaat ttttagagat gttgatgtta gttacaaaat tattctggaa
     1321 gaagattttt ataaggaaga tagggagtta caacaagatt cgattcaagt atcagggttt
     1381 atatttaata gattatatta ttatggatta ataagcaaag tagaaggata catggtacta
     1441 aaatctagta gtaagtttga gtataatagt actgtaggct taattttgaa gtgagcatat
     1501 gtttaaatat aatatacctt tagacattga aaatacaaaa agtactactg taagcccact
     1561 gtaaaaacta ttttttaaaa ggaaaaataa gttgtgagat agagtgtcta caagtgtata
     1621 tagataataa atttgaggtt taagattaac taagtgggtt tcaggtacat cgcgaaaata
     1681 attataagtt ggagattttg tatgtttaag attaactaag tggatttcaa atattgatat
     1741 agtagatata atagaagttt tattattagg tttaaaatta actaatcgga tttttaaata
     1801 ttctgactat tatataagga tattattagc ttgaatttaa aaccaataat ataaaacaac
     1861 aaaatattat atttatgata taataaaagt tatggaaaaa caggaatatt actatagaga
     1921 agagaggtaa acttatgaat aattggatag aagtaactat aaaaacaaca acagaagcag
     1981 ttgaacctat aacgaatata ctatatgaac aaggagctgg aggagctgta atagaagacc
     2041 caaaagactt tttattccaa aagaaaaatg aactagactg ggattatgtt gaagaagaag
     2101 tatttaaaaa gaatgaagaa gatgatgttc ttataaaaac ttatgtttct gaagaaaaaa
     2161 atgtgatgga atttgtcgaa ataataaagc aaaaagtttt aggattaaaa gattttggta
     2221 tagatatagg tgaaggaagt gtatcattgg accaagtaaa tgaagctgat tgggcaaatg
     2281 catggaaagc atactacaaa ccaacaaaag taggacaaag ggtagtagta aagcctacat
     2341 gggaagatta tgctatgcaa gatggggatt taatcataga gcttgaccct ggaatggcat
     2401 ttggtacagg gacacatgaa actactagta tgtgcattag agaattagaa aagtatgtga
     2461 ataaagattc taaagtattt gatattggat gtggtagtgg aatccttgca atagcagcag
     2521 ctaaattagg agcaaaagaa gttgtagctg tagatttaga tgaagtggct gtaaaagttg
     2581 caaaagaaaa tgttcttgaa aataaagttg aaaaaagtgt atctgtaatg catggtaatt
     2641 taactgatgt aataaaagat aaagcggatg taatagttgc aaatataata gctgatataa
     2701 taaagatact tgcaaaagat gttcaaaact ttatgaaaga agatgctata tttatatctt
     2761 caggtataat tttggataaa gtagaagaag ttaaagaaag cttaattgaa aatggatttg
     2821 aaatagttga agttcaaaaa ttaggtgaat ggtcagcaat agtttctaag cttaaaaaat
     2881 aagggatatg gtgacgtata atggatagat tttttgttga aaaaaataat ataaatctac
     2941 aagataaaac ttgcactata gaaggtgaag atgtaaaaca tatttcaaaa gttttaagat
     3001 gcaaactagg tgagaaactt gaaatatgtg acaagaataa taatgagtat atttgtgaga
     3061 taatgaatat agataagagt atagttaatt tagagatttt agaaaaagtt gatataaata
     3121 gagaatcaga acttaaagta aggttatacc agggacttcc aaaagctcca aaaatggaaa
     3181 tgatacttca aaaattaact gaagtgggtg tagaagaaat aatattagtt caaactaaga
     3241 gaagtgttgt taaagttgat gacaaaaaag aagataaaaa atttgaacgt tgggaaagaa
     3301 taatttatga agcagcaaag caaagcaaaa gaggtaagat accaaaatta agaggtgttt
     3361 taagttttaa agaagcttta gaagatatga aaaaaaataa tgtaaatatt tgcccttatg
     3421 aaaatgaaag aacagtttca ataaaacatg cactaaaaaa atgtgattct aatatagata
     3481 gtgttggaat ttttataggt ccagaaggtg gattttctga agaagaaata gagcaaatac
     3541 aaaaaaataa ttgtaatgtt gtatcattgg ggcctagaat attaagaact gagacagctt
     3601 ctgttgtagc atcaacaata gctttgtatg aactaagtga tttaggagga gaaaaataaa
     3661 ttgaaaaaag tagcttttta tacacttggg tgtaaagtta atcaatatga aacagaagct
     3721 atgctagaac tatttgagaa agatggttat gaacaagtca atagtgaaga gtatgctgat
     3781 gtttatgtta taaatacatg tacagttact catatgagtg atagaaaatc tcgtcaatat
     3841 ataagacgag ttaaaaagaa gaatccagat gcaataatag ccgttgtagg atgttattct
     3901 caggtatctc cagaagaaat tttggatata gaagaagtaa atttagtcat gggtacaaat
     3961 gatagaagaa agattgttga agaaattaaa aagataaatt caagtaagaa agttagtaca
     4021 gttgatgaca taatgaaagt taaagctttt gaagaaatag aaatcagcca aactaatgga
     4081 aagactagag catttatgaa aatacaagat ggatgtgaca gattttgtac ttattgtatc
     4141 attccttatg cccgaggacg cgttagaagt agagatattg atagtatagt cgatgaagtg
     4201 aaaaaattgg ctaataatgg ctataaagag gttgtattaa caggaataca tgttgcctct
     4261 tatggaaaag atttaaagga tagggatatt aaacttcttg atgtaataaa acaaataaac
     4321 cagatagaaa aaatagaaag aattaggctt agttctgttg aaccaatttt atttacagat
     4381 gaatttgtaa atgaagtttt aaagatggat aaagtttgtc ctcattacca tttatcactt
     4441 caaagtggat gtgatgaaac tctaaaaaga atgaatagac gatacacaac tttagagtac
     4501 aaaactattg tagatagatt gagaagtaaa atgccagatg tggcaataac tacagatgta
     4561 atagtaggtt ttcctggaga gacaaatgaa gagtttaaaa aaacgtacga gtttttaaaa
     4621 gaaatagagc tttcacaaat gcatattttt aaatattcac caagaaaagg aactccagct
     4681 gcaactatgg aaaatcaagt tgaccctcaa atgaaacact ttagaagtga acaactttta
     4741 aatttaagta aagtaaattt taataaattt gcaactaaat ttatcggccg tgagctggat
     4801 gttttgtttg aacaaaatat agaaggcaat aaatatgagg gattaacttc aaattatata
     4861 agagttgttg ttgaaagtga caaaaatata caaggtcaga tattaaaagt gaaaataaat
     4921 gatgtaaaag atgaatatgt agaaggaatt ttgctataac atgtagaaat tataatattt
     4981 ataagatagg aggtggaaag aatggattgt attttttgta aaatagcgaa cggagaaata
     5041 ccttcaacta aagtttatga agatgataga gtcttagctt ttaatgactt gaacccagtt
     5101 gctccatatc atatattagt tgtacctaaa aaacattatg atagtttaat tgatattcct
     5161 gataaagaga tggacattgt gtcacatatt catgttgtta ttaataaaat agctaaagag
     5221 aaaggttttg accaaactgg attcagagtc ataaataatt gtggaagtga tggagggcaa
     5281 gaagttaaac atttgcacta tcatatttta gctggtaaaa agcttcctaa ttacgaagcg
     5341 ggacaaaact aaactaaatt attgaaaaat tgagttcctt cttgataaaa atacttgata
     5401 aaagtaaaaa tttagtttat aataaacaaa gtgttaggtt gtaatgcata ttatgcaaaa
     5461 agaacatagt tacagtccca gcataattgc gctattggag ggagggaaaa agaaatgtca
     5521 gaagtaagag ttagagagaa tgaaacatta gacagcgctt taagaagatt caagcgtcaa
     5581 tgtgcaatgt ctggcatcat gtcagaagtg agaaaaagag agcactatga taagccaagc
     5641 gttaagcgta agaaaaaagc tgaagcagca agaagaaaaa acgctaagaa atagtagtaa
     5701 ataagaataa agaggtgaag gggatgtccc ttaagcaaaa gttacaagag gatttgaaat
     5761 cttcgatgaa aaacaaagat acagtgagaa aatctgtagt aactttaata agagcttcta
     5821 taaagcagta tgaggttgat aatagggtcg agcttgatga agatggaatt atcgatgtaa
     5881 tagcaaaaca attgaagcaa cgtagagatg cattagttga atttgaaaaa gctggaagag
     5941 aagacttaat aaaagaaaca gaaggtgaaa ttgaagtttt aaaagagtac ctacctcaac
     6001 aattaagcga agaagagtta gaagaaattg taaaatctac tatatccgaa gtaggggcaa
     6061 cttctatgaa ggatatggga aaaataatgt cagttatcca accaaaagtt aaaggaagag
     6121 ctgacggaaa acttataaat aagttggtaa aacaaaactt acaatagatt gatatagcct
     6181 agagagaaat ctctaggctt tttatagttt ttgggaataa cgagtcatat ttgagcatat
     6241 ctataatcaa agaaaatttt gaaagaaggg gaaaaatttg attatatcag taaaagagat
     6301 taggaatttt ttgataaaca tatttatacc actaataata ggttacttaa gtagcatctt
     6361 aacaatgatt atcagtggaa cagatatttc aacctattat ttacaactca caaagccaag
     6421 ctttgcacca ccagctttta tctttcctat agtttggact atattgtaca tattgatggg
     6481 tatatcttca tacctaatat taagaaaggg ttacaactta cctaaggtaa gagatgcaat
     6541 tttttattat ggattacaac ttgtattaaa ctttatttgg agtatactat tttttggatt
     6601 tggacttaga tttacagctt tgatagatat aataataatg attttggttt ctataataat
     6661 gatttcgaag ttttctaaaa tagataagag atctggatac ataaa
//
LOCUS       DAEPOZ010000197         6695 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.197, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000197 DAEPOZ010000000
VERSION     DAEPOZ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6695
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.197"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..1060
                     /locus_tag="KR440_002979"
     CDS             <1..1060
                     /locus_tag="KR440_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBH0699989.1"
                     /translation="RISGENCELEEKNKQLEESLNYEEHEIIKLQDSIATEEKEKENL
                     TKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQHIELKESLFKVGGRLERLKT
                     SQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESLKREIRSLGNINIGSIKEYEE
                     IKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFEIKFEEISKNFKYVYKRLFGG
                     GCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGGEKALTAISILFAILITKPTP
                     FCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHRRGTMEAADYIYGVTMQEKAI
                     SKVISLKLKEAQEITDII"
     gene            1087..2367
                     /gene="ftsY"
                     /locus_tag="KR440_002980"
     CDS             1087..2367
                     /gene="ftsY"
                     /locus_tag="KR440_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBH0699990.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDSLENLEETIFSG
                     LEEEVIDKVEDIEEKTEESVNNEGTEEIENFEKIEVEMDSNAKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            2470..2838
                     /locus_tag="KR440_002981"
     CDS             2470..2838
                     /locus_tag="KR440_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBH0699991.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            2835..4187
                     /gene="ffh"
                     /locus_tag="KR440_002982"
     CDS             2835..4187
                     /gene="ffh"
                     /locus_tag="KR440_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBH0699992.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPVNIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMAELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            4225..4497
                     /gene="rpsP"
                     /locus_tag="KR440_002983"
     CDS             4225..4497
                     /gene="rpsP"
                     /locus_tag="KR440_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBH0699993.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            4562..4789
                     /locus_tag="KR440_002984"
     CDS             4562..4789
                     /locus_tag="KR440_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBH0699994.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            4897..5412
                     /gene="rimM"
                     /locus_tag="KR440_002985"
     CDS             4897..5412
                     /gene="rimM"
                     /locus_tag="KR440_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HBH0699995.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVSREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            5421..6116
                     /gene="trmD"
                     /locus_tag="KR440_002986"
     CDS             5421..6116
                     /gene="trmD"
                     /locus_tag="KR440_002986"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBH0699996.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVYIYNIRDFSNN
                     KHKKVDDYPFGGGVGMVMTPQPIYDTYKHIITTHNISNPSVIYLTPKGKVYNQSIAKQ
                     MSLKEDIILLCGHYEGVDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            6301..6651
                     /gene="rplS"
                     /locus_tag="KR440_002987"
     CDS             6301..6651
                     /gene="rplS"
                     /locus_tag="KR440_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBH0699997.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
BASE COUNT         2855 a          668 c         1342 g         1830 t
ORIGIN      
        1 aagaatatct ggtgaaaatt gtgagctaga agaaaagaat aaacaattag aagagtcatt
       61 aaattatgaa gaacatgaaa taattaaatt acaagattct atagccactg aagaaaaaga
      121 aaaagaaaat ttaacaaaac aactagggga tagtaataga aatcttgaaa ctagaaaaat
      181 agcaaaagat gatttaaaaa atagttttga tgaaattaat aaagaattaa aaactataga
      241 tagacagcat atagagctta aagaaagttt atttaaagtt ggtggaagat tagaaagatt
      301 aaaaactagt caagacacat atataaataa gttgtttgag caatatgata tgactttagt
      361 acaagcattg gaaattaaag atgaagattt ggatatagat agaaaattct tagaaagtct
      421 aaagagagaa ataagaagtc ttggaaatat aaatataggc tctataaagg aatatgagga
      481 aataaaagaa agatacgatt tttatagcga acaaaagcaa gatttagaag agtctatgga
      541 agagatagaa aaacttatac acactttaga agaaaatatg aaatctgaat ttgaaattaa
      601 gtttgaagaa ataagtaaaa attttaaata tgtatataag agattatttg gtggaggttg
      661 tggagaatta actattttag ataaagaaaa tttattagaa agtgatatac taataacagc
      721 tcaacctcca gggaaaaaaa tgaaaaattt aaatctatta tctggtggag agaaagcttt
      781 aacagcaata agtatattat ttgctatatt gattacaaag ccaacaccat tttgtatatt
      841 agatgagatt gaagcgccac ttgatgatgc aaatatattt agatttggag aatttttgaa
      901 ggatttatct aaagagactc aatttatatc agtaactcat aggagaggaa ctatggaagc
      961 tgctgattat atctatggag ttacaatgca agaaaaagcc atttcaaaag ttattagctt
     1021 aaaattaaaa gaagctcagg aaataacaga tattatataa cttagattga cataggagga
     1081 ataacagtgt taaaaaaatt atttggtttt ggaaaagaca aggaaaaaga aatagaaaaa
     1141 aaagatgctg aagaagaaat agaagttgaa gacagtgttg atagtttaga aaacttggaa
     1201 gaaaccattt tttcaggact tgaagaagaa gtgatagata aagtggaaga tattgaagaa
     1261 aaaactgagg aaagtgtcaa taatgaaggc actgaagaaa tcgaaaattt tgaaaaaata
     1321 gaagttgaga tggatagtaa tgcaaaagaa gtagaaaata tttcaaataa tgattctcaa
     1381 gaagagataa tcgaagaatt agaaaattat gatgaaattg agtctaaaga gatagaagat
     1441 aaagaagata agaaagttaa cttgtttgaa agactaaaac aaggattgac aaaagcaaag
     1501 caaggaataa ctgatagaat agatgaagtt ttaaaatcat atacaaaaat agatgaagaa
     1561 ttacttgaag atttagagga aattttaata acagcagatg ttggtgtaaa tacaactatg
     1621 gatataattg aaagactaag agataaaata aagcaaaaag gtattactga accaataaag
     1681 gtaagagaag aacttaaatc tatagttgaa gatattctta caaatgaaaa ctctacacta
     1741 gatatagaac cagcaccatg tataatatta atggtgggtg taaatggggt cggtaagact
     1801 actacaatag gtaaactagc aaatagatac aaaaaagatg gtaaaaaagt tttattagca
     1861 gcagcagata catttagagc agcagcaaca gagcaattag aaatttgggc aaatagaacc
     1921 aatgtagaca taataaaaca tcaagaggga gcagaccctg gagctgtagt atttgatgct
     1981 ataaaagctg caaaagctag aaaaacagat gtattaatat gtgatacagc aggaagatta
     2041 cacaataagg ctaatcttat gaatgaactt ggaaaagtat ttaaaatagt agatagagaa
     2101 tttccagaag caaaacgtga agtacttctt gtggtggatg ctacaacagg tcaaaatgca
     2161 gttgttcaag caaagacatt taaagaagtt gcagatataa caggtatagt acttacaaaa
     2221 cttgatggaa ctgcaaaagg tggagtagtc cttgcagtaa aatcagaagt agatgtacct
     2281 gtaaaactta ttggagttgg agaaagtgta gaagacctac aagatttcaa tgcaaaatca
     2341 ttttcagatg cattatttgg taactaatgt tcttttaaaa ataatggttg acaagagtgt
     2401 tgttatgata taaaatacaa tagtgtaaag taaataacct ttacagtata ctgctaggaa
     2461 gtgtaagata tgaatataga aaaaatggtc gaaattggat tgttatttga acagtataaa
     2521 gaattattaa ctgacaagca gaaagaaata gttgctctat actatgaaga agattattct
     2581 ttaggtgaaa ttagtgaaaa tctgaatgta tcaagacaag gtgtttatga taccttaaag
     2641 cgttcagaaa aaatactaag agattatgaa gaaaagttac acttagtatc aaagattcaa
     2701 gagcaggaaa aaaatattaa gattataaaa gataaaatta ttgacattaa agaagattta
     2761 ttgcacaata gagattgtgc taatttaatc cctaagctag aaaatataga agatgtatgt
     2821 agggagatga taaaatgata tttgaaggat tgtctgataa actacaaggg gcatttagta
     2881 agttaaaatc taaaggaaaa cttacagaag tagatgtaaa aaatgctatg agagaagtta
     2941 aacttgctct tcttgaggca gatgttaact ttaaagtagt taaagatttc ataaaaaaag
     3001 ttcaagagag atgtgttgga caagaagtta tgcaaagctt aactccagct cagcatgtaa
     3061 tcaaaattgt aaatgaagag cttacaagtt taatgggaga tgtacaaagt aaagtaatga
     3121 tttcctcaaa gcctcctaca attttaatga tggttggatt gcaaggtgca ggtaagacta
     3181 ctacagcagg aaaacttgga ggatatttta aaaagcaagg taaaaaacct ctattagtag
     3241 cttgtgatat ttatagaccg gcagctataa agcaacttca agttgttgga gaaaaattgg
     3301 atatacctgt attcaatatg ggagataaag aaaatccagt aaatattgcg aaagcaggtt
     3361 taagccatgc gattaaaaat gctaatgatt tagttattat agatacagct ggtagacttc
     3421 atatagatga agtacttatg gctgagttaa agtctattaa atcagaagtt aagccacatg
     3481 agatactttt ggttgtagat tctatgacag gtcaagatgc tgtaaatgtt gcagaaagct
     3541 tcaatgaggc acttggtgtt gatggagtag tattaactaa attggatgga gatactagag
     3601 gtggtgctgc actttcaata agagcagtta cacaaaagcc tataaaattt atggggatgg
     3661 gagaaaagct agatgatata gaacctttcc acccagatag aatggcatca agaatattag
     3721 gtatgggaga tgtcttaagt ttaatagaaa aagcacaaga aaatatagac ttagagaaag
     3781 ctaaagaatt agagcaaaaa attaaaaaac aagagcttga ttttgaagat ttcttacaac
     3841 aaatggaaca aatacaaaat atgggacctc ttgacaagat acttggtatg atacctggta
     3901 tgggaaatgt aaaagaccaa cttggagatg tagatttgaa tggcaaagaa atgaagagaa
     3961 caaaagccat aattcagtca atgactacat atgagagaag aaacccagga gtattaaatg
     4021 cttcaagaaa gaaaagaata gctaagggca gtggaactag tgtacaagat gtaaatagac
     4081 ttataaaaca acttaatgaa atgaaaaaaa tgatgaagat gtttgctggt tcacaaaaga
     4141 gtatgaagaa aaggggcggc ttaggcggtt tacctttttt taaataaaat ataaaaaagt
     4201 aaaaattatt cggaggtgac tgtaatggca gttaaaataa gattaaaaag aatgggagct
     4261 aacaaaaaac ctttctacag aatagtagta gctgattcaa gagctccaag agatggaaaa
     4321 tttatagaag aaataggata ctataatcca atttctgaac ctaaacaagt aagaataaat
     4381 gatgaaaaag ctataaaatg gttagctact ggtgctcagc caacagaagt agttaaaaaa
     4441 ttacttgtaa aaaacggagt aatagaaaaa ttcgaagctt ctaaacaagc aaaataattt
     4501 tttcttggca attagtttaa gtaagggctc attgctaagg ttactttagg agatgaaata
     4561 tatgaaagag ctagttgtag atatagctaa ggctctagtt gataatcctg actcagttgt
     4621 tgttgaggaa tttgaagata atgatggtat cgtcttaaag cttacagttg ctcaagagga
     4681 tatgggtaag gttattggga agcagggaag aatagctaaa gctataagaa ctgtcgtaag
     4741 atctgttgca aatagagaaa atataaaagt ttctcttgag atagtataaa ataggaggat
     4801 taggtataat gcctagtcct ttttatataa atgcgtagtt taagatttta tgatgatatg
     4861 accacataag attaaatttt ttaagaaggt gataacatga aagaaaaatt aactcatttt
     4921 aaggtaggtc aaatagtaaa tacacaaggt ttaaaaggtg aagtaagagt ttatccactt
     4981 actgatgata tagagaggtt tgatgaatta aaagatttct atctgggaaa agatttaaat
     5041 aataaattgg ctgttgaaaa agtaagatac aaaggttcta tggttataat gaagataaaa
     5101 ggcatagaca gcatagagaa agcagaaaaa ctaaaaaata aatttatgta tgtatctaga
     5161 gaagatagca gagagcttga agaaggcgaa ttttttatag cagatatgat tggaatggaa
     5221 gttttaactg tagatggaaa atatgtagga atactagaag atgttcttca atactcagca
     5281 aatgatgttt atgtagtaaa aggtgaagaa gataaagaat ttatgatacc agctataaaa
     5341 aaatttgtac ctacaataga tattgatgaa agaaaaatga ttatagaccc tattaaggga
     5401 atgatagatt aggtggtaat atgagatttc atataatgac actttttcca gaaatattta
     5461 attcgtacat ggatgaaagt ataatgaaga gagcagttga aaaaggtata attgaggttt
     5521 atatatataa tataagggat ttttctaata ataaacataa aaaagtagat gattatccat
     5581 ttggaggagg agtggggatg gtaatgactc ctcaacctat atatgacaca tataagcata
     5641 taataacaac tcacaatata agtaatccaa gtgtaattta tcttactcca aaaggtaagg
     5701 tatacaatca atctatagca aagcagatgt ctttaaaaga agatataata ctattatgtg
     5761 gccattatga aggagtagat gagagaatca ttgatttaat agttactgat gaaatttcaa
     5821 ttggagatta tgtattaact gggggagagc tcccagcact tataatgata gattctatat
     5881 caagattaat accaggtgta ttaaaccaag aagaatcatt tgaagaagaa tcatttaaag
     5941 ataacttatt ggaatatcct cattacacta gacctagaga ttttgaagga ttaaaagtac
     6001 cagaagtgct tttatcaggg aatcataaaa agatagatga gtggagaaga gaagaatcta
     6061 taaggataac caaagaaaga cgatttgatt tatataaaaa gtctaatgaa aaataaaaat
     6121 ataatgaaaa attaatatat atgtatacaa taaagtacct ataaatggaa ttaaaaacat
     6181 aaaagtgctt gtaataacta ttgaagtata gtataatata aaatgttgaa aatacgggcg
     6241 ttcctctgtt tacactggtc gggtagttaa gaacgtctat gatatctagg aggaagaaag
     6301 atgaatgaaa tgctaagagc aattgagcaa gaacaattaa aaaatgaagt tcctaatttt
     6361 ggacctgggg acacagttaa agtacacgtt agaattatag aaggaaaaag agaaagaata
     6421 caagtattcg aaggtgtagt attaaagaga caaggtggag gagcaagaga aactttcaca
     6481 gttagaaaaa tgtctttcaa tgttggtgta gaaagaactt tcccagttca ctctccaaaa
     6541 atagaaaaaa tagaagttac tagaaaaggt aaagtaagaa gagctaaact aaactactta
     6601 agaggtagag taggtaaagc tgctaagata aaagaagcta gaaataaata attaaaaact
     6661 ttacttataa atggggggct gatttatcag tcctc
//
LOCUS       DAEPOZ010000198         6675 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.198, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000198 DAEPOZ010000000
VERSION     DAEPOZ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6675
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.198"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(213..899)
                     /locus_tag="KR440_002988"
     CDS             complement(213..899)
                     /locus_tag="KR440_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBH0699998.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(948..1481)
                     /locus_tag="KR440_002989"
     CDS             complement(948..1481)
                     /locus_tag="KR440_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBH0699999.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDA"
     gene            complement(1525..2157)
                     /locus_tag="KR440_002990"
     CDS             complement(1525..2157)
                     /locus_tag="KR440_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HBH0700000.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYNTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(2247..3326)
                     /locus_tag="KR440_002991"
     CDS             complement(2247..3326)
                     /locus_tag="KR440_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBH0700001.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(3459..4805)
                     /gene="pepV"
                     /locus_tag="KR440_002992"
     CDS             complement(3459..4805)
                     /gene="pepV"
                     /locus_tag="KR440_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBH0700002.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELDKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(4874..6154)
                     /locus_tag="KR440_002993"
     CDS             complement(4874..6154)
                     /locus_tag="KR440_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HBH0700003.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(6281..6541)
                     /gene="hfq"
                     /locus_tag="KR440_002994"
     CDS             complement(6281..6541)
                     /gene="hfq"
                     /locus_tag="KR440_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBH0700004.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
BASE COUNT         2139 a         1201 c          679 g         2656 t
ORIGIN      
        1 tttacatcaa aagtaatcat tgagttttaa cctccccatt agtttcatca ataaaataaa
       61 tttaatataa actttataat cataatatta aacttattta tactataatt attaatttga
      121 tgataaaagc tattttcctt ctaaataaaa aatttgtcta aagtttctaa aaaatttaga
      181 caaatcttag gttattattc tttaaattat ttttaaatta ttccaaatac accaaataat
      241 attggtataa atatagctgg taacaaatta gctactttta tattcgttaa tttaagtaca
      301 tttagaccaa gtcctataat taataaactt ccagctgctg tcatttctgt gactacagga
      361 tcacttaaaa atccatttaa aaatgatgct cccacacata taataccttc atacaaaaat
      421 acagatattg cagaaaacat tacacctata cctaaagaag cagtaaatat aattgaagct
      481 attccatcta taacagattt tacaaataaa gtatcatgac ttcctgtaag accactttct
      541 aatgaaccaa ctactgccat ggctcctata cagaataata aactagatga tataaaaccc
      601 tctgctatag aatctttttt atttccttta gaaaatttac tttgtaaaaa agcacccaac
      661 ttatttaccc atttgtcaat atctattaat tctcctatca gagcacctac agctacggat
      721 attattgtca ctaatgtgtt ttttccttct agtgctcctg atataccaat gtacaataca
      781 cataaggcaa ttccattcat tatagtatca cttactcttt gaggtaatcc tccttttatt
      841 ataagaccta ctatacatcc acctgcaatt gctagactat ttgctattac tccaaacatt
      901 ttttctcccc tcgattagct aattgaaatt tatcctatta ttaaaaccta agcatctata
      961 ctttgtattg gatattttaa atattccaaa aattcttcaa aaccttttct agatatattt
     1021 acagttttag tatttatgtt aggatgaaaa tttaagtaat cttctttaat tacatcttta
     1081 tctatgtaaa gctcaacttt tctttctgta tcatttatta atgaaaaagg atttacactt
     1141 ccaggaagaa gtttaagtac attgtatagt ctttcaggtg aagcaaacga taatctagaa
     1201 cttcctatct tatctttaag tgagtttaaa tcaacttgtt tttctccttt aactacaata
     1261 agataatact tatttccttt ggcatttctt aaaaataaat ttttacattg acaccccttt
     1321 gttattccat ctaaaatgac taattgctct gctgtatata cagcttcatg ctctactaca
     1381 tcatattcaa catttaattt atccaaaata tcatatattt tagtttcatt catgctagat
     1441 tcattttcat cttgttcttt tttattattt aaatcactca tacttttctc cttaaacact
     1501 tcatcataat atcattgtga ggtattattc tactatagct attgctctta ctggagctcc
     1561 atcagcatca ttatacttaa atggtgctgc tataaataga aatttttctg gaacattttc
     1621 taaatttgta agattttcta tgattatttt tcctttttca aataaaatat gatgcacttc
     1681 aaatactgat gtgttatatc tatccactga tagcatatct attcctatgc cttttatatt
     1741 agtattagct atatagtttg cagcttcttt tgttaatgta ggataaccta catagtactg
     1801 ctttttatcc caaaattttg accaacctga tttaaatatt ataaaatcac tattttttat
     1861 ttcatcttca tattttttta gatattctaa ttctatattc tcatttccct tttcaagttc
     1921 caatacataa gctttcccaa caaaattttc tatatccaac ttatctaggg tctcaccttt
     1981 agtatacata tgtcttggtg aatccatatg agttccattg tgagaaaata cactaattaa
     2041 tgtttcttga taaccatttt cttcaataat agctactttc ttgatatctg gtctattagg
     2101 ctctgcatat acaggcatgt cattatgtgt cacatgagtt aaatcaaaaa ccttcatctt
     2161 tatatacccc ctatgttttt attttctatt atatacctaa attcatttta tttctatcag
     2221 attttattcc attatatacc atactactat atatttttta taaacctcaa gggatttacc
     2281 gaaaaatctt ggaaagaatt atcagtaaat ataacatctt cacccaaaaa acgtttatac
     2341 cttccttctt ctttctttaa agtaattctc tgtttttgtg aatgattatt aaaaaccatt
     2401 atcttaagag gaggtttttt tctattagtc tgcactcttt ttaacatgta ttttatgtgc
     2461 atatcagctt caggaaatga atatccacaa aatattaaaa ggtcagtatc tcttaaagtt
     2521 ttttctgttt cattccaaac attacttaaa aatatgtttg acatattctt aaaatatgtt
     2581 ggtggcacta taataggttc tgttaactca ccacactcta aacattttgt ctctgaacta
     2641 ttttcaataa gcttcatgac accaccttca tgtggtgtaa gagttacact attacaaatg
     2701 gggcaataaa gccaattcaa tgacccatgt atcttatata atttgattaa tggttctttt
     2761 ggttttctcc aatttaactc ctctttaaaa tttgtaaact ccacaccata atcaagcata
     2821 attggcaaat cattcttatc tcttaatcca gtaactgtat tgtctatatg tatatcataa
     2881 tttgcactaa taaaagtagt atcaagaagc aaatcatttt ccaaaagatt ttcaactaaa
     2941 agtttatgat atttattatc tgttctaggt gtattatgaa ttgcttcagc cattagcagt
     3001 attaaatatt gtcttaaaaa acgtatacta tcacttctgt tatttagatt ttctagtcca
     3061 aaatttctaa atgcttcttt tctttgttct gctaagtcta aaagacctaa aacttcttca
     3121 aaggtaggaa aatttgcatt atctatatta tcttttacta tatcaatatt gaacatctgt
     3181 ttaaagaaaa tatacaattc tctattcatt ttggtattat aatttgtggg atttagtttt
     3241 ttaaaatatt cgctaaaaag ttcattttga ataggtagat tctctgctgc tgaagctcct
     3301 gctcctaaaa atattgctat tttcatatag ttcctcctaa tataaatatt actcctattt
     3361 ttatatccat taaatatcca tatcatttat tttatgatac aacaataaag ctgtctttta
     3421 ctagaaaaaa tcctattaca agacagcctt atattatatt aaatctttaa taattcgtaa
     3481 atagaaagag catatatttg agtagctttt ataatatcct ctactattaa gtattcatta
     3541 gcttggtgtt ctgtttctgg ttttcctgga aaaatagctc caaatgcaac acaattatct
     3601 aaagctcttg cgtatgttgc tcctccagat gaaagtggtg ttccatcaag accagtttct
     3661 tcttcatata cttttctaag tgtcttaaca agaagagtat cttctggaac atagattgaa
     3721 tccaagaaat catattcttc ataatttaaa ttgtatttat ctgtcatttt ctttaactct
     3781 tttacaaaat catccttttt gtatgttact ggaatcctta catctattcc tgcaaattct
     3841 ttttcgttat caatagttac tctacctata tttactgtta gtttacctga tacatcatcc
     3901 ttgcaattag gaattatatt gtttccatta gcatcttcac caataacttc tgctaaaaac
     3961 ttaattatat tagaatctat acctatatta ttaagagcta tacaaagtct agccactgca
     4021 tttataccag tatcacttgc tgctgaatgg acactttttc caattataca gattttatta
     4081 ccttcaacag tatactcaaa atttaattta tcaagttctt tttttaattt atcagagtat
     4141 tttccaatat agattgcttt acctggaact gcatttaaag cctttccaac tgacaactct
     4201 atatcacttt tgctatcaca agtcaaatga acttgtaaaa gacctttctc tgcatttgtt
     4261 attgggaatc tagaatctgg agtaaatcca tagtttggta tttcttcatt attttcttta
     4321 tatttattta tacatctcca aagattttct tcatcagccc caaaaatgaa tcttatcctc
     4381 ttattaaagt ctacatttaa atccataaga gctttcactg cataaattgc tgatattgta
     4441 ggtcctttat catcttgtgt tcctcttcca tacaatttac catcttctaa aacagcttca
     4501 aatggagggt aattccagct ttctaaatca ccttctggaa ccacatcaac gtgaccaagt
     4561 atacctatca ttttttcacc ttgtcctatt tctgcatatc cataatatcc atctttgtat
     4621 actgtcttaa atcctaaaga tttacataag tctaatatac ctcttaaagc tttatctaca
     4681 ttttcaccaa atgggcaatt ttcagttgct tcagatataa cactaggtat ttttacactt
     4741 tcttgtattg aagaaatcat ttcatcttgt agagaattta ctttttcttt aatttgctct
     4801 ttcatattat cacccttcaa ttttcatcat aatatactaa taaatatttt gaccttttta
     4861 aaaaaaaata tgcttatttt ttcagttttt ttataaattc aatcgctttt aaagtcccca
     4921 tttttgaatg gtcaaatgta agtccacctt gaaaataaac attataagga ggtcttattg
     4981 gagcatctgc acttaattct atagaagaac cttgtataaa tgctcctgct gccattatga
     5041 cttcactttc gtatccaggc atgtcccaag gaacaggttt tacataagaa tcgactggtg
     5101 cagcttcttg tataccttca caaaaactta ttacttcatc agcattatta agtctaatac
     5161 attgaataat atcacttctt aagtcatcat attttggtaa tacatcatat cctaattttt
     5221 caaaagctct tgagcaaaag atagctccca ttacagcctg agatactata taaggcgcca
     5281 agaaaaaacc ctgaagtaca tttctagttg ttccaaaagt aagaccacat tcttttccta
     5341 ttccaggaga agtcattcta taagatataa gctcaatcaa atcttttcta ccagctatat
     5401 atccacctgt caatgcaagc ccaccaccag gattttttat taaagagcca gccataacat
     5461 ctgctccaac atcagtaggc tctttggtat ctaaaaactc accataacaa ttatcaacca
     5521 ttactatagc ttctggttta acagacttta ttacttctat agcttctttt atatcactaa
     5581 ttgaaagtga ttttctccaa gaatatcctt ttgacctttg tatcataacg agtttagttc
     5641 tatcatttat tttgttttta attccttcta aatctaagtt tccatcctct aaaaaatcta
     5701 catcatcata tgtaacacca tactctttca atgaaccttt ttcttctctt atgcctataa
     5761 ctccctctaa agtatcatat ggtctaccag taacagataa aatttcatct cctggtctaa
     5821 ctataccttg tatacataaa gtaagtgcat gtgtaccatt tacaattatt ggtcttacaa
     5881 gagcatcttc tgtattaaat actttagaat aaatttcctc tattttttca cgaccaatat
     5941 cattatatcc atatcctgta gtccagttaa agtgcatatc acttagttta gattcttgca
     6001 tagcttttaa aactttatat tgattatatt ctcttatttc ttttatttct tcaaacttat
     6061 cttttatttc ttccattatt tcttgcgata gtttaaaagt atcatcatct attccatagt
     6121 agtcttttaa taattctttt gtctcattaa gcattttatc acctgtctct ttattatttt
     6181 atttaaacat gtaaaggaga ctattttact aaaacaaata aattatagtt tataagttag
     6241 tataagtctc ctctcaatca tcttaaatta aattaattat ctatctgttg ttattattat
     6301 tgttgttttg gttttgattt gttagattaa tatactttcc aggttgtata gttgatacag
     6361 catgtttata aatcatgttt tgttgtctat tatccccttc tattaatatt atataactat
     6421 caaacccctt tacaagccct ttaacttgca ctccattaac taaatatata gtaacaggta
     6481 tcctttcttt ccttgcatta tttaaaaaca aatcttgtaa atttaaaact gtatttttca
     6541 ttagatttgt accctcccaa gattaaattg aacttctaaa atattcctac cttatattct
     6601 atataagtta tgacaaattt tcaatattaa gttgtatact taaaaattta tatattattt
     6661 tatagaatct ttaat
//
LOCUS       DAEPOZ010000199         6663 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.199, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000199 DAEPOZ010000000
VERSION     DAEPOZ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6663
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.199"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(15..266)
                     /locus_tag="KR440_002995"
     CDS             complement(15..266)
                     /locus_tag="KR440_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700005.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIIGTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            complement(297..1316)
                     /locus_tag="KR440_002996"
     CDS             complement(297..1316)
                     /locus_tag="KR440_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HBH0700006.1"
                     /translation="MFKKCIKVVTLTFILACILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEENTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDKY
                     FKQKN"
     gene            complement(1334..2287)
                     /locus_tag="KR440_002997"
     CDS             complement(1334..2287)
                     /locus_tag="KR440_002997"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HBH0700007.1"
                     /translation="MINVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            complement(2630..3406)
                     /locus_tag="KR440_002998"
     CDS             complement(2630..3406)
                     /locus_tag="KR440_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0700008.1"
                     /translation="MKFHEFGDKNKPHIMLIHGGGNAWWNYLRQAEVLSNNYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDEI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKM
                     FQSYVPSCEIYEAKGYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            complement(3440..3961)
                     /locus_tag="KR440_002999"
     CDS             complement(3440..3961)
                     /locus_tag="KR440_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0700009.1"
                     /translation="MDIAKLILNPARLRILQYIRLHGSARTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVVDNDKTK"
     gene            4560..4826
                     /gene="rpsT"
                     /locus_tag="KR440_003000"
     CDS             4560..4826
                     /gene="rpsT"
                     /locus_tag="KR440_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBH0700010.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
     gene            complement(5013..6056)
                     /gene="holA"
                     /locus_tag="KR440_003001"
     CDS             complement(5013..6056)
                     /gene="holA"
                     /locus_tag="KR440_003001"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBH0700011.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(6010..>6663)
                     /locus_tag="KR440_003002"
     CDS             complement(6010..>6663)
                     /locus_tag="KR440_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HBH0700012.1"
                     /translation="YCIMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIIL
                     IMNNPYVIYNVSFQLSFLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFK
                     GIPLLSIIGNLIIVPFVGVIMYLSIASLIVFKVSVVIAKIISFFNSTLIESIFFLLEK
                     ISNLSFAYINIESPKFYIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
BASE COUNT         2208 a         1082 c          725 g         2648 t
ORIGIN      
        1 ttattcactt attactactt attaaatttt tttatgattt catgtatatc aaaatttggt
       61 atgtcaaaat caaattttgt tatatcaaat ttaatacttg gaaaatcata ttgtttcatt
      121 cctagcatac tttttgcatt actgtctact ataaatacac aaactgatgt acctataatc
      181 atagtaatca aaaataagat tctcataata tacttgtatt ttcttagttt acttttactt
      241 tctatatttt tttctaatcg gctcatttag catgctccta attataactt aaagctctaa
      301 tttttttgtt taaaatattt atctaaaatc tcggctaaat actttgtagt agccttagat
      361 tcttctacac ttgttgcgtt attccctatt tctactaaca tatgattctt aactgcaaac
      421 tgattgaatc tagcatagtc tttttctact attggagata ctagaccagg atactttttc
      481 tcagcaaaag cagttatatc ttctgctaaa gctctaagtt gttttctatt tgtattttta
      541 cctcctacaa caaagaagaa ctttgatact ctctctccat ttatcatagt tgtatatttt
      601 aaatggtctt tttccttagt ggctggatta gttaaatccc tagcatctct atgtaagtct
      661 atcgctatat ccactgagtt atatttaggt aatatacttt gtattgtttt taagctactt
      721 gcataagagt tattataaga tggatagtca tggtatttag tagtatggac tacaccccat
      781 cctttgctat ctaaagcact agttaatgca cttcctactt ccattactga atttttctta
      841 tctcttgaat gataatttcc atcaggtgaa tttgaataag tctcacatcc atgagtatga
      901 tatatcaaaa ttcttggatt ttctttggtt actcttaaat catccttata atctgaacta
      961 gatgttgtgt tagtttcagt tgtgtctgaa ttttttgact caatatctgg aacattttct
     1021 ttaccaacat aaagttttat atattctttt tcactctctt ttttattatc aactttactt
     1081 gcatctttag atttagtgtt ttcttcttta ctttcttcct tattttcttt gcttgtttct
     1141 ttatttttat tattcttttt attttctgta tcatttcctt cgactttagc ttctggataa
     1201 gatgaattta ctaaaaactt taaaaaatca tcctgattca aagctaatga ttttcctggt
     1261 aaaatgcatg ctaatataaa agtcaaggtt actactttaa tgcatttttt aaacataatt
     1321 gctcctcctt attttatacc aatctacctg gatgtaaaga cctatttaag ccttcactta
     1381 ttattataga tagattttcg attgtatcat ctatatcctt tggtgttact attaagttct
     1441 tgtcatatgg gtctaacgaa tcttttatta gatgatattt ttcttcttct ttaagttttt
     1501 tcaacatttc ataaaaactt tctgtttctt ctgattgagc tattaaattg tctattgcca
     1561 tatctaagac atctattgta agagttgctg catcaaccac cgttggaacg cctattgcaa
     1621 taacatccac gcctaaagtt tctttagtta gagattttct agtattgcca acacctccac
     1681 caggtgatat tcctgctgtt gatatctgta tagtagagtt tactctttcc atttttcttg
     1741 aagccaatgc gtctattgct actactctat ctggttttac tttttcaacc aatgacttta
     1801 ctatttcact agtttctatt ccagtaacac ccataactcc tgggcttaaa gctgatacct
     1861 cagtgaaatc atcatcatag tctttattat aatttttaaa aagatgtcta gttacaagtg
     1921 tttttgatac actctttggt cctaacgcat cagaagtaat atttctattt ccaagaccaa
     1981 ttaccagggt ttttttattt ttgtcttggc caaatatatc tactaactcc tcttttaaat
     2041 aacttatcat ttcttctctc gactcatcat catcaaactt cataagatta ctttccagag
     2101 ttatatactt tcctatatct ttattcatta tctctgcgcc ttgcctgtct attacttcaa
     2161 ctttagttac aatgcaattt tctaattctt tagtttctat ttttacacca ggaatactac
     2221 ttccttcttg agacttttca cacatttcac tagcttctaa agctaaatct gttcttacat
     2281 taatcatata aacctccagt ataaattaaa tgctaattat aattgttaac aaaatttagt
     2341 attttataac aagaaattgt aaatttattt aaaacaagta aaattaaggc tttcaatgta
     2401 agaaaattgg gtttagtcag tgatttgtca gtaaactttc ttatctatca tttatttaca
     2461 agcaaaaaaa ggtagcaact atttatatag gcgctacctt taatctattt ataattttcc
     2521 tattaagctt tcactaatat attaactaaa ataacttttt tatatttata cctaatttat
     2581 ttactatata aaaaaacagc aataattata caataatcac ctaacacctt tattcctttt
     2641 ggaaaaatgg ttctgcaatt tccaaccatt catttggaag ataaagcgat aaatacccat
     2701 gattatagcc cttagcttca taaatctcac aagagggtac ataagactga aacatttttg
     2761 cagatttttt aacgcacttc atttcttttt ctccatacca gtacattact tgtgctgtgg
     2821 tttttttaac attctcttta agtgtatagt tcatcatata agtacgatac atattataga
     2881 atgtttcttt tcttacatgt ggcatatctg tcatataata cgcttttatt tcatctggat
     2941 attgcatatt ttcaggtaac aattttggca tcatagcaat ttgaaagcga caagccttct
     3001 cactaaacaa aagtcctcca aaaaatcgaa taccagcaat acaaaacctt gccataagag
     3061 gtcttgggta gcaaatacta ccatcaataa ttgctttttt agtaatatta ggttttcgag
     3121 ataaaagttc catgacaatt tgaccaccaa gtgatacccc acacaaagcg aaaagatgtc
     3181 cactgcaatt ttcatctata tatgatatca acttatctgc tgtatcctct gtagaaatgt
     3241 atgttgtttc atattcttct ccatgaccat ccaatgtagg aagtatcaca tgatagttgt
     3301 tagacaaaac ttctgcttgt cgcaaatagt tccaccacgc attccctccc ccatgaatca
     3361 acataatatg tggtttgttt ttatctccaa attcatgaaa tttcatatac aacctccttt
     3421 tataaaaata agattcatgt tattttgttt tatcattatc aaccacaggt ggagaagata
     3481 taattgaaag ttttctaagt tttagatttg atttaggctt ttgttctata tatggtttca
     3541 ataaatcagc tatactttta agaagatttt catattcatt atcactcata tatagtaatg
     3601 ctgaagagaa aaatacatta tcctttttat ggtcttgctc ttttctgcta aagtattcat
     3661 tcatttcctg catcagacct acataaaatg ctgtagaaag tgcaacgaag gtctctcctt
     3721 ccatagatgt tgtatattcc tttagtgcat aaaccttttc aatagtacca cgcacacgat
     3781 tttctttaat tacttcaatc atattgttct cttctaaaat ttttacatgg tgataaactg
     3841 ttgcacgagg aatatcattt aaatacttta caatatcaga tgtgcgagca ctgccatgaa
     3901 gtcgaatata ttgtaagata cgaagccttg caggatttaa aattagtttt gctatgtcca
     3961 tcttatcagc cctttcttta gtctataaaa atattatagt ctattttttt agactattca
     4021 agtctatgat ataagtagta attacaacta taataaatgc tacaaatgct attctatata
     4081 aacactgaga ttaattactt ataaccttct aacatattag ttatattgtt tgatgcatct
     4141 atcatacata ctaatgaaaa aaattatctt gattattgga aaaattatct taattaagca
     4201 tataaattta tatttttata atttttcatt aaatttacaa aaatgagtaa tttcttgtat
     4261 tttcttataa ttattggaaa tactatcaaa atgtatgtta atagtttgta ttatctcaaa
     4321 atatttgtta caaaaataaa atattaattt atatatgtca aaatatttaa aacacaaaat
     4381 ataccaattt aaaaacaaga tattgcaaat tatttataca caatttacag tctaaaaaat
     4441 caaaataaaa aacattatta aaatatataa ataataatcc ttgattttta gatttatttc
     4501 tgttaaaatc taatatgttg acgatgaata tgataaaaaa atacagggag gtgaacacgg
     4561 tggcaaatat aaaatcagct aagaaaagaa taagtgttat cgaaaagaaa actgctttaa
     4621 atagagttag aaaatctcaa ataaaaactg caataagaag attcgaagat gctgttgcag
     4681 ctggaaacag agaggatgct gttgcaaagt tccaatacgc tcaaaaaaga atataccaag
     4741 tagcttctaa aggaactata cataagaatg ctgcagctag aaaagtagct aaattagctc
     4801 aaaaattaaa cggtatgaac gcttaattac cagtttatat ttcttatata taataacccc
     4861 tatgtagggg ttttttattt tgtaaattta tacaataaaa aataaatagt tagacatatt
     4921 atagttgatt gtatatcaaa cttaatttag cttacatcta ctctgaacta taacatatct
     4981 tgctatttat tttttattaa tatatagatt ttttaaattg cttctctttt acagtatcta
     5041 ctaaccaaca tttcaactgc taaagtatct cttataagac ctgtttttat tttataatca
     5101 ctttctaata tatagtttaa tatttccaca attatatcat cagaaaaatt tttagtttgc
     5161 tttaatgctt ttcctacaac aaattgatgc atttttaatt tatcagctat aagttttgtt
     5221 gaatatccat caagttgtaa ctgtcttact tgcataatta ttttaaattg tcttgctatc
     5281 atagaaaata tacctaatac agattcgcct tcttgtatca tatcatttaa tattttcatt
     5341 gcattagaag cattttgttc tccaatataa tcgattaatt taaatatatc attctcaact
     5401 tttttttgag ataatttatc aattacgtca ttggttacat tattcccttt accaacaaag
     5461 gaacttattt tatttatttc attttctaaa tcagataatg tcttttcact gctcttatct
     5521 ctgtatcctt cttgttcaat aaaatacatg atttgagaat tatctattat aacatcattt
     5581 aaagcaaaag atttttttat ccatttaaat aaatccatat cagaaagctt atcacaatca
     5641 aaaacaattc cattgtttcc tattttctta actaatgatt ttcttttgtc tacatctcca
     5701 tatacaacaa aaactatagt tgtagtgtct ggaatattat ctaaatgctc tattaagtat
     5761 ttctcatcac tatctgtaaa attctttttc tttcctttta atagttcgaa gtcttttatt
     5821 attactatct ttctatcatc cataaatgga agtgtttcta tagagcttat aagctgattt
     5881 aagactattt ccttcccatc tattatatct aaattaaaat caagcattcc ttcgtttaga
     5941 ctatctttaa atgcttttat tgcattctca attaaataat actctcttcc ataaaaaaga
     6001 tacatttttt caaagttttt ctcttttaca ttccttataa tatccttgta attcattctc
     6061 ttgctccttt atctcttttc cttctatgta aaatatgtaa aaaaacactc ctatgtaata
     6121 aataacaaca atataaaatt taggactttc tatattaatg tatgcaaaac ttaaattgct
     6181 gattttttct agtaaaaaaa atatgctttc tatcaatgta ctattaaaaa aagatataat
     6241 tttagctatt actacactta ctttaaatac gattaaactt gcaatactta aatacattat
     6301 tactcccaca aatggtacta taattaaatt tcctataatt gaaagcaatg gtataccttt
     6361 aaatgtataa taaattatag gtaaagttaa tatatttgat gatattgtca atgataccat
     6421 acttaatttt ataatactat ttattttatt atagaatatt aatatagaaa gtgtagctaa
     6481 aaaagacaat tgaaaactta cattatatat aacatagggg ttattcataa ttaaaattat
     6541 cccaattaga gacaatgctg atattccatc tttctttcta tcaataaata ttgataaata
     6601 aaaaatcata acaaatgcta tagacctaat tatggatgga gacgcaccta ccattataca
     6661 ata
//
LOCUS       DAEPOZ010000200         6637 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.200, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000200 DAEPOZ010000000
VERSION     DAEPOZ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6637
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.200"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..1129
                     /locus_tag="KR440_003003"
     CDS             <1..1129
                     /locus_tag="KR440_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0700013.1"
                     /translation="YYLKELIKLKTKDKKATIKNIDKNKLQDIMFEFFREIDKKYNSN
                     FLEDKEFFNLFYLHIACLIERIKKNHKIINPFSVKISQQYPTVFNLSIQFSKIIENEY
                     HIKISQDEIGFIATHIAVPFEKREEANFNKKYKIAIICSSGGGSAFLINLRLKEIFPN
                     AEIRNFSLLEEKAVIEFAPDLIFSITNLLFEINAPVILINEILDELDYLDIKESVRFA
                     DHIGNISSPKQYILGLFDKNHFRCIKDKAKYKDILDSMSKCIVDEGACSPTYPKDVWE
                     RESYLSTIYTNGVAIPHPIEMTGNKNIISVALIQTDIIYENRVPKIIFMISLIKGNLE
                     LHKQISKYLSKIMANKDMVDMLNKSQSYEEFMYKLKIYLGG"
     gene            1132..2175
                     /gene="ypdE"
                     /locus_tag="KR440_003004"
     CDS             1132..2175
                     /gene="ypdE"
                     /locus_tag="KR440_003004"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBH0700014.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNQKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            2190..2495
                     /locus_tag="KR440_003005"
     CDS             2190..2495
                     /locus_tag="KR440_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBH0700015.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPTMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            2592..2927
                     /locus_tag="KR440_003006"
     CDS             2592..2927
                     /locus_tag="KR440_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0700016.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            2959..4281
                     /locus_tag="KR440_003007"
     CDS             2959..4281
                     /locus_tag="KR440_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBH0700017.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILNNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEDLLDDLGLDF"
     gene            4291..5361
                     /locus_tag="KR440_003008"
     CDS             4291..5361
                     /locus_tag="KR440_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBH0700018.1"
                     /translation="MNNTIERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIELIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            5366..6553
                     /locus_tag="KR440_003009"
     CDS             5366..6553
                     /locus_tag="KR440_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH0700019.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVANNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
BASE COUNT         2562 a          736 c         1239 g         2100 t
ORIGIN      
        1 gtattattta aaggagctta taaaactaaa gactaaagat aagaaagcca ctataaagaa
       61 tattgataag aataaacttc aagatattat gttcgaattt tttagagaga tagataaaaa
      121 atataactct aattttttag aagataaaga gttttttaac ttattttact tacatattgc
      181 atgtttaata gaacgtataa agaagaacca caagataata aatccttttt cagttaaaat
      241 aagtcaacag tatccaactg tttttaattt atctattcaa ttttcaaaaa tcatagaaaa
      301 tgagtatcat ataaaaataa gtcaagatga aatagggttt atagctactc acattgctgt
      361 tccttttgaa aagagagaag aagctaattt taataaaaaa tataaaatag caataatatg
      421 ctcttctggt ggaggtagtg catttttgat aaatttaaga cttaaagaaa tttttccaaa
      481 tgctgagata agaaattttt cattactaga agaaaaagct gtaatagaat ttgcaccaga
      541 tttgattttt tcaataacaa atcttttatt tgaaataaat gctccagtta tacttataaa
      601 tgaaatttta gacgaattgg attatttaga tattaaagaa agtgttagat ttgcagacca
      661 tataggaaat atatcaagcc caaaacaata tatattaggt ctatttgata agaatcactt
      721 taggtgtata aaagataaag ctaagtataa ggatatcctt gatagtatgt caaaatgtat
      781 agttgacgag ggagcttgtt ctccaaccta tcccaaagat gtatgggaaa gagaaagcta
      841 tttgagtact atttatacaa atggggtggc tatacctcat ccaatcgaga tgacaggaaa
      901 caaaaatata atatcagtag ctttgataca aactgatatt atttatgaaa atagggtgcc
      961 caaaattatt ttcatgattt cattaataaa gggaaattta gaacttcata agcaaatatc
     1021 taaatattta agtaagatta tggcaaataa ggatatggta gacatgctaa ataagtcaca
     1081 atcttatgaa gagtttatgt ataagttaaa aatctatctg ggaggttaga catgaatata
     1141 gagtttttta agaggttgtc agaagcagat ggtatagcat ctaatgaaga agaggttaga
     1201 catgttttat tggaagaact aaaggcttat agtgataaaa ttatctgtga tgggctaggg
     1261 agtatcatct tttctaaaat aaaagatgag agtgcaccaa acgtaatgat ttgtgctcat
     1321 atggatgaag ttggatttat ggtgagaagt atagacaaac ttggtatgat tcacttaata
     1381 acaataggag gagtaaaacc tcttgcacag tttgtacaaa aagttagaat tacaacaaaa
     1441 gaaggaaaga aaatccctgc tgtcataaat gctacatata ataatggtaa ggctgaaaat
     1501 atatatgcag atattggagc atatacagaa gaagatgtat ataacctagg gattaatgtt
     1561 ggggatatgg taacatatac aacaagtttt gaggaattta ctttaccaga ccgattagtg
     1621 ggaaaggctt ttgatgatag gataggatgt tttgtgatgg gagaagtttt aaaagaactt
     1681 aggaaagaaa atctaaattg taatattcac tttgcggcta caagtagtga agaggttgga
     1741 ataaggggag caaagacatc aacacagtta ataaatccag atatagtatt tgtaatagat
     1801 gtagcatgtg ctaaaaatga gtttgtgaga gactatacaa atcagaaaca aattgataaa
     1861 ggtgtaatgc taatgcatag agataggact ctagtcccta gtagaaaaat gatagattat
     1921 gtaatggaaa ttgctgataa aaataatata ccacttcaac atgatatgtt tgaatcaggt
     1981 gggactgatg gaggagaagc acatctcgta aatgaaggga aaccttgtgt agtaacttgt
     2041 gtaccagtta gatatggtca ttgtgcattt tcaatagtaa gtaataaaga tttagagaac
     2101 attataagat tgtatacaca gttaatttta aattttgatg aagaacaata taaacatata
     2161 agaaatttta tctaaaggag aataagaaaa tgtcaacaga agtacaaata atggagctta
     2221 tatcaactgc aggagagagt aaatcgaaag cttttgaggc tttaaaaaaa gtaaagtcaa
     2281 aagattttaa aggagcaaaa ttgttaatag aggaggctcg taaaatagat attgaggctc
     2341 acagaataca gacagaatta ataactcaag atttaaatga agatgttgaa agcccaacaa
     2401 tgagtctttt aatggtacat gctcaagacc actatatgac ttctcagcta gcaaaagact
     2461 tgatagaggc tttgatatat tgtctcgata agtaattgta atgtagaaat agattattta
     2521 tgtaaaaatt cttttaaaag tttagatgct tttaagagat aataaaaata caaactttag
     2581 gaggaattat tatgaaaata ttattatgtt gtgcaggtgg attttctact aatatgttaa
     2641 tgcaaaatat gaaaaaggtg gtaaaagaga gtgctaaact caatgaaaaa gattttgagt
     2701 ttacagctat accagcagat tcaatagaaa gtgaagttga tgattgggat atagtactta
     2761 taggtcctca aatagctcat aaaatagact tcttaaaagg aatattagag ccaaaaaata
     2821 agccttttgc agtaatagat aaagatgtat atggaaaaat ggatggagct acagttatga
     2881 agttggcatt aataacttat aagaaacatc aattagctca aggataataa aaataatgag
     2941 ggaaaaggga gattagatat gttttcaaaa tttgaagcat ttatgaatag agtatttatg
     3001 cctttagctc ataaggttga caatcaacgt caccttggtt ctataaaggc tggtatggtt
     3061 gctatgacac cattcacaat attaggaagt atatttgcaa tacttccagc acttccaaat
     3121 atgcttggag aaaataatcc aataagcaaa ttcatactta acaattcagc aatattggat
     3181 ttaccagtaa gtctttccat aggtttaata ggattatatg cttgtatgtg tattgctttt
     3241 aatctgggga actactataa attatatata ccaggttgtg ttgcactatc aactttttct
     3301 tttttattcc tagtagcctc atttacacca gatggagctt tagaaatagg aaatttagga
     3361 gctaaagggc tatttacagg tatgatagca gggcttggtt ctgtggagtt atataatttt
     3421 tgtaataaga agaatatcac aataaaaatg ccagaaaatg tacctgattt tgtatctagg
     3481 tcttttgaat taatacctac tactataata gtttgtggaa cttttatagt tgctcgtttt
     3541 atttcattaa aagtatttgg agaattacct ccacagatac ttactcaatt tttatctcca
     3601 ttagtaggaa gtatggataa cccatggtca gttcttgcac ttaactttgc tatatgtact
     3661 atattctttt ttggaataca ttcatcagta tttagtccta taactagacc tataatgatt
     3721 acatttatag ctgaaaacat agcagctatg caagctggag aaacaatacc tcatttcttt
     3781 acagcaggag cttcaagtgc attctttgga tttacaggat gtggaattag tataggttgt
     3841 gtaattgcat gtatgttatc aaaaaatgct tcctataaga agattggacg tgtatcatta
     3901 ttccctgcat tatttgggat aaatgaacca atattatttg gagcaccgat aatattaaat
     3961 ccaataatgt ttataccaca tgtatttggt ggagctataa taggtacact accaatgttt
     4021 tttatgcact ggggatggtt agcaaagcca gtatttgacc ctccatatgt aggggtattc
     4081 ttagaaggat ttttaactaa tggagattgg cattctatat tagcaaatgc attgcaatta
     4141 gtattagcag ttttattata ttggccattc tttaaaataa tggaacgtca acaaaataat
     4201 aaaaaacaaa aagaagaaac taagaatata ttctctaaag aagaagaaga cttattggat
     4261 gatttaggat tggatttcta atattttgtt atgaataata caatagagag attagtatcc
     4321 ttaatctata aaagaggtgt tgacgctctt ttgattaaag caaaaaatac taaaagatat
     4381 ataggagctt tatcaggtag tggtgtatat gtgcttgtta ctaaggataa aaattatcaa
     4441 attctagatg gcagatatat tgatgaagca gataaaaaaa cgactggttt cataaaaagg
     4501 attgtatctc aaggttctta tataccaact ataattgagt tattaaaaga attaaatata
     4561 aaaaagttag caatagaaaa ccaagctatg agtatacaag agtatctttc actaaaaaat
     4621 gaaggttttg aaatcaattt aataacaaat gaattatgga agctaagggc tataaaatct
     4681 aaagaagaaa ttgaacttat taaaaaggct tgtgagatta cagatgaagt atttgatgag
     4741 gttatttctg aaattaaaaa agatatgact gagctagaag tatcagctct aattcagtat
     4801 catgcattaa aaaaaggtgc aagtggaatg tcttttgaaa caatagtggt atcaggagaa
     4861 agaggtgcaa tgccacatgg aagacctaca aacaaaaaat tgaaagtgaa tgaagctata
     4921 acaatagatt ttggtgttgt ttatcaaggg tatcaatcag acatgactcg tacaatatct
     4981 ataggtaaac ctcctaaaat tattaaagag atatatgatg tagtattgga agcacaatta
     5041 tctgcaatag agtcaataaa ggaaggaact agggcaagtg atgttgacaa agtggctaga
     5101 aaaattatag ataaacatgg atttggcgaa tattttaatc atggactggg tcatggtata
     5161 ggtcttggag atggtgaagt tcccacttta aatccaaata gtgaagatat attagtggaa
     5221 ggaatggtaa tgtcttgtga gccaggtatt tatattccaa atgtaggggg agttagaata
     5281 gaagatgata ttgttataat agatggaaaa ggtgttccac tcaataagac atccaaagag
     5341 tttataatat taggagagta aaattatgaa atataatttt aataaagtat taaatcgtaa
     5401 agaaggaaat tgtagaaaat ggagcaacca tgtgattaaa gaaaaattcg gcttaaatga
     5461 agatgcaatt cctatggatt tagctgatat cgattttgag tgtgctcctg ctataaaaga
     5521 atctatctta aaaagagcat cagtaggaga ttatagttat acttttatag gagatgactt
     5581 ctatgatgct attataaatt ggaataaaag aagatttgat gtatatatag aaaaggattg
     5641 gataaaatta acttttggta ctgtttcaac ccttcattat atagttcaag catatactaa
     5701 agaaggagag ggtgtattga taaatacacc tgcttatgac ccttttgcag aggctgtagc
     5761 aaataataat agaaagttgt gttgtagctc attaaaatta gataacaata gatattattt
     5821 agatttagag gatatggaaa atcaaatgaa atacgagaat ataagattac ttatattctg
     5881 tagtccacaa aatccatcag gtcgtatatg gacaaaagaa gaactatatc aagtgtcaga
     5941 gttatgttta aagtataatg taattttggt aagtgatgaa attcatagag atataatatt
     6001 taaagattat agatttgtat ctttgtggaa tgctcatcca gaaatttata aaaatagtat
     6061 aatctgcgta tctcctaata aaggcttcaa cttaggtgga ttaaaaacat catatgtatt
     6121 aataagagat gtaaaaatta gggagacttt attagaaagg ctaaaaagta attcaataac
     6181 ttctccaaat gtatttgcaa taccagcaat agttgcagca tacaatgaaa gcgaagaatg
     6241 gctagatgct atgactagtt atgtagagga gaattttgaa atagtttatg atttctttga
     6301 aactaatata ccaaaagcca aagtgatgaa gtcagactct tcttttttag cctggataga
     6361 tgtaagagaa gtatttagaa atgaagaaga atctaaagaa ttttttagac atgcaaactt
     6421 aactatggta gttggaagtt attttgtgaa agatggagat ggatttattc gtattaatgt
     6481 ggggtgtcct agagaaacat taaatgaggc tctaaataga atcaaaaaaa cgtatataag
     6541 tatgtattgt taaatcataa aaaaagtatc tatggtttat ataaaaacac attatttagt
     6601 tatcaaatat tactggaatt ttgtattcca gtaatat
//
LOCUS       DAEPOZ010000201         6635 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.201, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000201 DAEPOZ010000000
VERSION     DAEPOZ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6635
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.201"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            184..414
                     /locus_tag="KR440_003010"
     CDS             184..414
                     /locus_tag="KR440_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700020.1"
                     /translation="MRLDMDSMLYVLIYMTLLVSIIGSIFYFCKDLLNHKIKYVLIRD
                     IFVNRITYSEERLPKQYIVFQKYDIWRYCSLF"
     gene            471..1121
                     /locus_tag="KR440_003011"
     CDS             471..1121
                     /locus_tag="KR440_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBH0700021.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEHIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRMYFNRKNRIVTK"
     gene            1335..2006
                     /locus_tag="KR440_003012"
     CDS             1335..2006
                     /locus_tag="KR440_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0700022.1"
                     /translation="MAKILVVEDEKRMQSIIVEYMQKGGYTCITANDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            2003..3133
                     /locus_tag="KR440_003013"
     CDS             2003..3133
                     /locus_tag="KR440_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0700023.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKFEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDAISHEMKTPLGIIRAYTEGLMDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLIINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRIIEQDEISKIWSKFYRSVQSQ
                     RKGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            3538..4383
                     /locus_tag="KR440_003014"
     CDS             3538..4383
                     /locus_tag="KR440_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0700024.1"
                     /translation="MIKVGELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSLFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            4383..5411
                     /locus_tag="KR440_003015"
     CDS             4383..5411
                     /locus_tag="KR440_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0700025.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDNYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFLICDLCFTVLLVV
                     FNIGFFEGVKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            5414..6427
                     /locus_tag="KR440_003016"
     CDS             5414..6427
                     /locus_tag="KR440_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0700026.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTML
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGAPLLIYLLGVN
                     TSGIGNLCYIMPSVATFNGIMEIINGNVIIIKDIVILVVHCVVWFMIYIILDKKRKLS
                     FSR"
BASE COUNT         2486 a          638 c         1151 g         2360 t
ORIGIN      
        1 aagattatat actttataaa tgtaaatatg acgaataaaa atgataaata taataatcta
       61 tatatttaca ttaggagttt tctatactat aattatttat atttaaaact aaatataaat
      121 gttggaatta tttaggattt gaaattgtct aaagataaat ataagtgatg aagaggtgat
      181 gtgatgagat tagatatgga tagtatgtta tatgtactaa tttatatgac attattggta
      241 tctatcattg gaagtatatt ttatttttgt aaagacctct taaatcataa aattaagtat
      301 gttttaataa gagacatatt tgtaaataga attacatatt ctgaagaacg gttgcctaaa
      361 cagtatatag tttttcagaa atatgatatt tggaggtatt gtagtttgtt ttaatttgaa
      421 atgaatctat tataaattct aaagtttaaa aataaaaagg agagaataaa atggatttca
      481 tttcaagtat attgcaagga ttattaagta tattatttaa ttttacaggt gattttggaa
      541 tttctattgt acttataact ttaatagtta aattagtact tatgccacta tcattaaaac
      601 aaagattttc tgtaaaaaaa cagcaagaat tagctgaaaa aatggaacat ataaaagaaa
      661 agtataagaa tgatactaaa gaattggaaa agcaaataca agtaagttct gtagaaagta
      721 tgaagagtat gctgggatgt cttatagttt tggttcaaat gccagtagta tatgctttat
      781 atcatgtatg tttagatatg acaaaggaat ttacaacaat tataattcca tggattgcta
      841 atttaggtac atttgataat ttgtttatat taccttgtat atatacatta attatgttag
      901 cttcaaattt agtgaattat ataccatatt taaaaataaa ttctcaagta aagtttaaca
      961 aacaaatggc tgtggtgact accataacaa gtcttatact aactgttaga actccagtag
     1021 ccataggtct gtactttata acaagtgcaa tttattcatt aattgaagat atttgtttta
     1081 gaatgtattt taatcgaaaa aatagaatag tgacaaaata atataattat tattagaaat
     1141 attttagcct gctaacatag aaaaagttag taggctattt ttatctatga tatcaattcc
     1201 attaagaata gtctaaaaaa tactatcaaa atgctataaa aaatactata aaaatactat
     1261 aaaaaaatag ttgaaaaata aataaattaa tataaactaa aataaagcct acaaaaaaga
     1321 ggtgataaat agaaatggct aaaatactag ttgtagaaga tgaaaaaagg atgcaaagta
     1381 ttattgtaga atatatgcaa aagggaggat atacttgtat aactgctaat gatggagtcg
     1441 aggcacttac aatattaaag agcaacaata tagatttaat gatattggat attatgatgc
     1501 cgtatttaga tggattttca gtgtgtagag tttcacgtga aatgactaat atacctatta
     1561 tcatattgac agctaaaggt gaagaggagg acaaattaaa gggttatgaa tatggcgcag
     1621 atgattatat aacaaaacca tttagcccaa aagtactttt agcaaaagtt aatgccttgt
     1681 tacgtagata cactactgat attcccaaaa attcattgag tttaggaaag atttttatta
     1741 tggtagcatc aaggcaagtg tatgtagaag ataaattgat tgatttaaca tataaagaat
     1801 ttgaattatt gcgtttattt atggaaaacc ctaatcaagt tttttccaga gagaagcttt
     1861 taaattgtat atggggatat gattttgaag gaaacacacg aactgtagat acacatataa
     1921 aaaccttacg caaaaagctt ggtagtgaag gacaccatat agttacatta attcgttcag
     1981 gatataagtt tgaggttaag gaatgaatag tttaagaaag attatagaaa aactgaaaaa
     2041 tgtagatatt tcaaattgga ttaaacaaaa ttttggacta tattcagtta gaaaaaaagt
     2101 gtttttactt tctaaattat caggagcgtt aattatatta ttttatctgg ttgttgaaga
     2161 attttcgata aactctaaat ttgaattttg gatttggttt ataatactgg ttatatttat
     2221 tatgagtatt gattttttat tgggaaaatt tatttcagaa ccaatcacat ctataaataa
     2281 atctgcaaaa agtatgtccc aacttgattt ttctaatcca tgtactgtaa atactaatga
     2341 tgaatttgga gaactttcta ggagcctgaa tacaatgtct acaaatctac aacaggcatt
     2401 atcagaccta gaaagtgcaa atattcaact tgaaaaggat gtaaataagg aaagaatgct
     2461 attggaacaa agaaaggagt tggtagatgc aatttcacat gaaatgaaga ctccacttgg
     2521 cattatacgt gcgtatacag aggggcttat ggatgaagtt gatgaagaaa aaaagaaaaa
     2581 ttatatgaat gttataatag aagaaactga tagaatgaac aatatgattg tatcattatt
     2641 agacttatct gcattggaag caggagtttc taaacttaac ccagagcgtt ttgattttat
     2701 agagtttgtt gaaactgtgg caggtcgtct tttaattgat ataccagatg ccaattttta
     2761 ttttacatat gatttgccag agtatgaagt ttttgtagtg gcagataaaa tgagaatgga
     2821 acaagtagta gagaatctaa ttattaatgc aaagaagcat gttgtatata atggtaattt
     2881 agatttatcg attacatgtg aaaatggctt gcttttattt gaaatttata atgatggtag
     2941 gattatagag caagatgaga tttcaaagat ttggtctaag ttttatcgta gtgttcaatc
     3001 tcagcgtaaa ggaggttcag gtctagggct tgcaattgtt tcacaaatac ttacaatgca
     3061 agggttagaa tatggagtga ataatcgtga tagaggagta gagttttatt ttatgattcc
     3121 aattgatgaa taatttagta ttaatttata aattataagt atctgatgta aaatattttt
     3181 ctttgtatac gtaagtattt aaagagaata aaataaaaaa cttaatgaaa aagtctaata
     3241 aaaagtttaa tgaaaagtct tgttttcctg atgaaaacaa gattttttat taagcttttt
     3301 attatttcac ttcgatttca caagagagac actctgattt caccaaatag aaataaaata
     3361 ggttaaataa attaaaaaat agaagcgtaa ttaaataagg ttacaagggg gagtacttat
     3421 gaaatggtat acatggttga ctattatatt gataatattg ctttctattc cgcttaaaat
     3481 taaatttata aaatggtggg atagacgaca aaaaaatgat aaaggaggtt atgatagatg
     3541 ataaaagtag gagagttaaa attttcttat ggaaaagata aacagatatt gcatggactt
     3601 aattttgaag taaaagaagg agaaatcttt ggattccttg gacctaatgg ttcaggaaaa
     3661 tccacaacac agaaaatctt aaatggcgtt ttaaaaggtt acaatgggca cgtatccctt
     3721 tttgggaagg aagttaaagc atatacagag tctctatatc aaaagattgg tgttttattt
     3781 gaatttcctt atttatatac caatttaagt gcaattgata atttagaata tttttcgtct
     3841 ttttatccta aaaatcaaag gagagacata tatgaactct tagatttgtt agagtttaag
     3901 aaagagttta taaataagcc tgtatcatcc tactcaaaag gtatgaagca acgtattagc
     3961 atggcaagag cattggtaag taatccaaag ttgttatttt tagatgagcc tactagtgga
     4021 cttgacccat ctggagctgt actatttcgt aagattattg aagaagaacg aaaaaaagga
     4081 acaactatct ttttgactac tcataatatg ctagatgcag atttaatgtg taacaatgtg
     4141 gcttttattg cagatggcaa gattatggta atagataaac ccaaaaattt aaaaatgaaa
     4201 aatagcaata ataaagttga agtggaattt gtatataatg gaaatcgaga tattgaaatt
     4261 gtagatatac aagagttaga atctggaata acttttaaat atgatgaaat cttaagtgta
     4321 cattcaaaag aacctacact agaagaagtt tttattaaat acacaggaag gatgttagtg
     4381 taatgtattc taaatttttt accttagtaa aaaaagattt ttggatgatg atttatggta
     4441 aattttttgt gctgacactt ggctctctca tactttattc gtgttatatt aattttggtt
     4501 atgtaaagtt tatggatgaa gatagttatc acaataatgt atatctatat aatccagaga
     4561 atataagcat gagtgcatct ccattaatta atcaagtttc ttctataggt gaattaaaag
     4621 caaaacttga tgaagataca agtgcagttt gtattgatat gagttccagt aagccgaaag
     4681 taattttaca tgaaagtatt aaaggagttg acaattatag agctgactat gcattgtcat
     4741 tattatcatc agaaaaagac cataatagca aaatatcaga caacaatgca ataattcaaa
     4801 ttaatgataa ggatacatct gagataaaac aaagaaaaga catgacttgt gagattttat
     4861 tctttgaaat tgtagctatt ggatttttag ggattgcctc tttattattt aaagaaaagc
     4921 agatgggagt aatgcgtatt actggtattt tacctgtaaa aagaagtctt tttatattat
     4981 caaagatatt tgcttttttg atatgtgacc tatgttttac agttctttta gtagtattta
     5041 atattggatt ttttgaagga gtaaaaatac ttccacaagt attagttcaa gctagtattt
     5101 tatcaattat tatgtctttg attggaatag gatgctcaat gttattgaga gattttaagc
     5161 aattttctct tatatatctt gtggttgctg tattcataac tacacctata tttctatctg
     5221 caaatacatc ttttgaaata gattggatta aatatcatcc cttctatcat gtttatagtc
     5281 agcttaaaaa tgcgtttttc atgaattttt caaaagaatt agtttatttt gtagtgtgtt
     5341 ctttgacaat aatagttcta tttttacttg taaaatatat ttttaataaa gagttggtta
     5401 gggagggata atcttgatag gattacgtta ccagttaaag agtataaaaa aagataaaat
     5461 gtgtataatt tccttctttt taccagttgt tatggcgatt ttaataaatt ttatgggaac
     5521 aattgatata tcttcattag gagagatacg ttttggtata gtgaaggata cacttcctgt
     5581 acaaacagag cagtggttga agaagtatgg tatggtaagt gtatatgaca caaaaatgca
     5641 tttaatacgt gaaattaagg atattaaaac ggacacaatt ggagttgtta gagatggtaa
     5701 aggaataaaa actatgcttt atggaaatga gattaatgct ataaaaaatg ttggaaagac
     5761 attgccagag atgtttagac taaagggtga tttaaaagat gttgaggtta caatattgcc
     5821 taaagatagt attttaaaag agtttcagaa tgtttttatt gctatgattt taattactgc
     5881 tatgtttatg ggatgtacct ttaatgcaat gaatattata tctgaaaaag aagatggaat
     5941 atcatttata aatgaagttt tgccacagag tagaagtcag tatattatac aaaaagtatt
     6001 tttgggtctt ttatgtgggt gtttatcttc aatgataact gtatttatat gtttggatat
     6061 ttcaatatta gatatgataa tgatgttgtt actcgttgta ttttcagcat ttatttcatc
     6121 acttataggt ttatttattg gaaagttgtc agaaggtctt atggtaggaa ttgtttatat
     6181 taaaatagta atgattttat ttataggtgc tcctttgtta atatatcttc taggggttaa
     6241 taccagcggt atagggaatt tatgctatat aatgccatca gttgctacat ttaatggtat
     6301 tatggagatt attaatggta atgttattat tataaaagat attgtaattt tggttgtaca
     6361 ttgtgtagta tggtttatga tatatattat tttagataaa aaaagaaagt tatcttttag
     6421 tagataactc agtataatta taattgggac aatttaatag gtgatttaat atactttgta
     6481 tagtgtaatt tatgtagtgc aatttgtata ctttaattta tgtagtgtaa tagttagttt
     6541 tattttataa ttattcaata taatttagta agaaattcag tacaattaga gctacttagt
     6601 ggaaaactta ataggtactt aaactatata aatac
//
LOCUS       DAEPOZ010000202         6551 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.202, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000202 DAEPOZ010000000
VERSION     DAEPOZ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6551
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.202"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(143..1588)
                     /locus_tag="KR440_003017"
     CDS             complement(143..1588)
                     /locus_tag="KR440_003017"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBH0700027.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINKRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            complement(1732..2640)
                     /locus_tag="KR440_003018"
     CDS             complement(1732..2640)
                     /locus_tag="KR440_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBH0700028.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            2897..3721
                     /locus_tag="KR440_003019"
     CDS             2897..3721
                     /locus_tag="KR440_003019"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBH0700029.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILINEFIKLNF
                     SNTFINTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRVFFNSTVVAIALIVYGIIMIGLEKRNKRPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            3761..4291
                     /gene="lepB"
                     /locus_tag="KR440_003020"
     CDS             3761..4291
                     /gene="lepB"
                     /locus_tag="KR440_003020"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBH0700030.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSVVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEILG
                     KVMVRLLPLDNIGKVD"
     gene            complement(4373..6154)
                     /locus_tag="KR440_003021"
     CDS             complement(4373..6154)
                     /locus_tag="KR440_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HBH0700031.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
BASE COUNT         2119 a          974 c          795 g         2663 t
ORIGIN      
        1 tatagctatt ttattaaatt catggtatag taagtaaaga agaactcttc acagcgctga
       61 agagaagacg ctagcccatt aaaatggcta tcttatttat aaataagata gccgttttta
      121 attatatttt atctgtacaa ttctatactc ttaggtccac atctactttt cctaagtctt
      181 ccttataatc ttttatagca ggttctacaa cttcatttac aaattctaca acttgttctg
      241 gagctctacc tataaagttt tttgggtcca ttatagatag tatttcttcc ttgctcattt
      301 taaatgaatc atcattcatt ataagttcaa ttaagttatt atccttacct tcgtgcttta
      361 ctctataggc agctttcata gaatattctc ttattatttc atgtaattcc tgtctatctc
      421 cacctctttt tacagcttcc ataagtattg tttctgtagc cataaaagga agttcttcat
      481 ttacacgttt atttatcata ttttcatata ctactaaacc atcagttaca tttatcccta
      541 tttcaagtat agcatctgct gccataaatg cttgaggtat tgccaatctc ttattggctg
      601 agtcatctaa tgttctctct aaccattgag tggcttgtac cattgcaggg cttatagatt
      661 cagagattat atacttagat aaagatgcta ttctctcact tctcattggg tttctcttat
      721 aagccattgc tgatgaacct atttgatttt tttcaaatgg ctcttctaat tcttttaaac
      781 tttgtaaaag tcttatatcg ttagtcatct tatgcatact ttgagctatc cctgatagta
      841 tgcttattac ttgataatct aactttctag tatatgtctg tccacttact gcataggaag
      901 aatggtatcc catcttttta caaattttct tatctaactc ttttactttt tcgtggtctc
      961 cttcaaataa gcttaaaaaa cttgcttgag taccagtagt acctttaact cctcttaact
     1021 tcatgttatc tattctatag tttaaatctt ccaaatcaag aattaaatct tgagcccata
     1081 aacaagctct ttttcctact gttgtaagtt gagcagcttg gaagtgtgta aatccaagag
     1141 ctggcatatc tttgtactta agtgcaaatt cttttaaatt gtttattaaa tttacaagct
     1201 ttactcttag aagttttaaa gcttcattca tttgtataac atctgtatta tctccaacat
     1261 atgcagatgt tgctcctaaa tgtattattc ctttggcttt ttcacattgt aaaccataag
     1321 cttttacgtg agacattaca tcatgtctta cttctttttc tatttttcta gcttcatcaa
     1381 aatttatatt atctatatta gctcttagct catctaattg ttcttcagtt atattgattc
     1441 ctaactcatg ttcaccttct gcaagtgcaa cccataacct tctccatgta gagaatttaa
     1501 attgtggaga aaatatataa ctcatgtcct tgctacaata tctgtctgta agtgggtttg
     1561 agtaagtagt atatttatta tctatcatta tattagcacc ttccttaaat cttttgtcgt
     1621 gtgattactg tgtacacatt agtacataaa taattatata taattttacg aactataaca
     1681 agactttttt attttttgtt cacatctata tatagtattt tttatttatt actatttttt
     1741 atttatatcc aaattatctt tttcaattaa gtaatccact atttcactat caacaaaatt
     1801 taatacatac ttatatctaa ttgcatagta tttatcactc catccaagct gattttttac
     1861 atcactttca tctgccccaa gccttaaact tattgctgca aatgaatgtc taaaatctct
     1921 tgctgaatat tgagaaagtc cagctaaatc taatgctttt ttgactataa gtctgacatt
     1981 tctatctgtt atagaattac tcttttgagt tgtaaaaata aaatcatcac taggtattat
     2041 tacctctgga agcccagaat aattcctgta atcttctaaa agtttaaaaa agtatggatg
     2101 taatttaact actctttctt ttcttccctt acctaacctt acatatgagt tatcgttttc
     2161 atctaccata aggtctttcc actttaagct aactaattca tttaataaac acccagtagt
     2221 aactagacag actattatta ctctatctct tatatttaat tttggtagga tacctataag
     2281 tttatttatt tcttgtatac ttagaacatc atcttttgtt tttattcttg ttactgtggg
     2341 tttttctaca tatctaaatg gatttatatc tatgtatcct tcttttttta agaagttata
     2401 aaaactatgt aaataactat atatcttttc acaagttgat ttcgcatatt tagtctgtat
     2461 atagtctaca aaatttttac aatcttcttt tgtagcataa atcaattctt tcccttttaa
     2521 aaattcttta aacagtataa cctttgataa ataatcgtgt ttcgtgtttg gatttaattt
     2581 tgtagaaaaa acattaaaag tttcatactc ataatcatct agtatttttg tactatacat
     2641 aataactcct ttccttttca taaaataaaa tagctatact tttactatac catatatttc
     2701 cccctcaaat atacccctta ttcaatagat aacttttaag tttattttgt tatattatga
     2761 aggttttatg aaaatatcaa tcttccattg tgcactacac agtactatgg tattataagt
     2821 tagtaaaagg tggcattttt ttattaatct atgaataaaa tatgctacta taaatttgta
     2881 ttggaggtat tttgatttgg aaacatttag tttgcttgaa atttttaagg cagtcatttt
     2941 gggcatagtt cagggaataa cagaatggct accagtaagt agtacagggc atatgatatt
     3001 aattaatgag tttataaaat tgaatttttc aaatacattt ataaatacat ttttagttgt
     3061 aattcaattt ggttcaatac tagctgtact tgttatattt tttagaaagt taaatccttt
     3121 tgatagtgca aagaatatta aacagaaaaa agaaacagtt cgtttatggc ttaaggttat
     3181 aattgcagtt ataccatctg gagttattgg aatactgttt gaagatgaca tagacagagt
     3241 attctttaat tcaacagtag ttgctattgc tcttattgtc tatggtataa taatgatagg
     3301 gcttgaaaag agaaataaaa gacctaagta taaagatttt tctcaagtta catataaatt
     3361 agctctttgt ataggattat tccagtgtct agctctcata ccaggtacat ctaggtctgg
     3421 ttctactata ataggtgcag tattacttgg tacttctaga tatgtagcag cagagttttc
     3481 attcttctta gccattccta caatgcttgg agctagtgca ttaaaacttc taaaagctgg
     3541 atttggattt actggatttg aatggcttat tttaggtgtt ggttctgtag tagcatttgt
     3601 tgtttcaatt gtagttatta aattttttat ggactatatc aaaaaacatg acttcaaagt
     3661 atttggttat tatagaatag tcttgggtat agtggttctt gcttatttct ttctattata
     3721 atgtattcta atttaataac aaatgataag gagaaaagtt atgagtgtta aaaaagaaat
     3781 atttgattgg attaagtcaa tagctatggc tattgtactt gcatttgtaa ttctacaatt
     3841 tataagacct tctgttgtaa gtggagaatc aatgtatcct actttagatg ataaagatta
     3901 tctgatttta aataggatat catacaaggt tggtaaacct gaaaaaggcg atattgtagt
     3961 ttttaaaacc aatttagttg atggagaaac aggaaagaaa aaagacttaa taaaaagagt
     4021 tatagctact gaaggtgaca gaataaaaat atcaaattct aaagtgtatg taaatggaaa
     4081 attattaaat gaaccatata tacacaataa ctatacttct ggagatatag atactgttgt
     4141 tccaaaaggt aaactatttg caatgggaga taatagagaa aatagtaatg atagtagatt
     4201 ccctgatgta ggtatggttg atgaagatga aattcttggt aaggttatgg tgagactatt
     4261 acctcttgat aatattggga aagtggacta aaaaataaga gactgaattg gtaaagttta
     4321 ttttacaaat ctcagtctct ttttattttc ttaaattaca aatatttttt tactaaatat
     4381 cttttgattc tgtaataagt cttattatat cacttgcttc ttcattaaaa gttgttacta
     4441 tacttaggta gtctgttgct ctactcatag ctaaataaac cttatttaaa tctcctacaa
     4501 agtcatttac ttgataatct tgttcaatat catttatagt ttgattgtat aacatctcta
     4561 attcacatac tattaccgct ttatactcta aattctttat tgcatatata tttgctattg
     4621 tagttcctac cttcttactt atatttgtca aattatcttc tgcacatata taaggaattt
     4681 ttgcctcttc caatgctttt tttaacatat attgaaagta aatggttttt ccacttttta
     4741 atttcttctt attataagga taaacgatag caacttcaga ataatcaaga ccttttttac
     4801 ttattaaata ctcaatttcc cagattacag aacttatttg gtcatcaagg tcacttactt
     4861 ttattatatc aacagactta tttccacctc taagtgcttt agtataaaag aaagtactaa
     4921 attctgtatt tggtcttaat tcgttgatat atgagttaga attatttgaa aatttatttg
     4981 taaaatctac aatctcttta gcttgtctat aatttttact taagataatc ttatcatcaa
     5041 attctatacc attatataat tttttaaata tattcaatct attagagata ttcaaggagt
     5101 tacaagaaca cacattaaat atgaattttg ttttatataa aaactccctt ataaaatcta
     5161 tttcatactc taaaaagctt tctgcttcat caataaaaat agccttgaac atatttttat
     5221 tttttataat gttctgtgct tgttttacaa gattattaaa ggtcttttcg taatcatttt
     5281 ttaacatgtt ataatctaca attaaattga attttttagc tagtttaaat ataaaagaac
     5341 taaatgtgtg aacttctaaa ttgttattat ctttgtataa taactctatc ctctctcgaa
     5401 gctcattgca caattgtttt gtatgtgtaa atatgactaa tttatgatgt ggatatactt
     5461 ttgctaattt tatagcccta gaaagcataa ttgctgtttt gcctatacca gaaccacctt
     5521 caataagtgt attcccatat ttaatagaaa caacatcttt aatttgaacg tcttccatca
     5581 tagttgctgt atacttatag tcatcattgt agaatgaaat ttttttaaat ttttcattta
     5641 gatgaagttt atcatttatt atatagtatt ctgaacatat gtctagtaaa aataaattta
     5701 agtttatttc atcattctca tcattaagat actcctccag cgaaccctta ttcattatat
     5761 cttgaagctt atttttatct attatattat tattaacaaa ttcttcaaat tcatatgttt
     5821 cctcaacttc aacgtatggc ataatgtaaa cgtagttata acttatattt ctattctttt
     5881 gtcccatttt tagtttcaaa agttcatatt cctcattcat aacttcaagt aactcttctt
     5941 ctaaaataaa aaatatatct tctgttgtat ccataaattt tataaataaa attcttccat
     6001 ctttaacata caacaaatcg gtatttattc ctttaaaagg agtaactttt ggcactactt
     6061 taacgtcctc attgatattt tcgtaaaata ttttctcgaa ctccttcaat tttagggcgt
     6121 ctaactgaga atttatattt atgtattggc tcacttacat tacctccttc tgttatactt
     6181 aattatacca ttttgaacta tatttttata tatacttttt ataggcaaat ttagtaaata
     6241 aattcaaaat aaatacaaat aaatacaaaa ctgctaaaat aattttgcaa taaatataga
     6301 tatttattgt tatagaatac tttctgtaaa taataaaatg ataaatttca taattaatgt
     6361 ttaaaatagt aagtaaagtt ttaaaaaaaa taaatcttgt tgactaaaaa taagatttat
     6421 tataaaaaat ttaaaaacct gctaaaaata atctattttc attagcaggt tttatttata
     6481 atcttcttat attaatacta ttatatccta tttttagatt ttactgtagt atttactatt
     6541 tattttgtag t
//
LOCUS       DAEPOZ010000203         6520 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.203, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000203 DAEPOZ010000000
VERSION     DAEPOZ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6520
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.203"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..331
                     /locus_tag="KR440_003022"
     CDS             <1..331
                     /locus_tag="KR440_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBH0700032.1"
                     /translation="INREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVMLRGIVDA
                     YFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEKCIYLFG
                     VDEAVCY"
     gene            377..1588
                     /locus_tag="KR440_003023"
     CDS             377..1588
                     /locus_tag="KR440_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBH0700033.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVIATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            1575..5102
                     /locus_tag="KR440_003024"
     CDS             1575..5102
                     /locus_tag="KR440_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0700034.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNIQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKNNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFVVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQAIKLEEELVLLDRELKDLKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRS
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHNITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVINITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            5233..6486
                     /locus_tag="KR440_003025"
     CDS             5233..6486
                     /locus_tag="KR440_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBH0700035.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
BASE COUNT         2901 a          590 c         1189 g         1840 t
ORIGIN      
        1 tataaataga gaaaaatcaa tttatgcaca agtaaatatg aaagacatat atatttatga
       61 aaagcttata aacaatgatg ataaaaaact ttatgataat gaaagtgtca tgttaagagg
      121 tatagttgat gcttattttg aagaagataa tcaaatagtt ttggtggatt ataaaacaga
      181 ttttgtaaat gaggaaaata taaatcaaat aatagagaaa tataaaaaac agttggattt
      241 atatgcagat attattgaaa ctttaactgg taagtcagta aaagagaaat gtatatattt
      301 atttggagtt gatgaagctg tttgttatta gtttttatat ataaattaaa cctaaaagat
      361 agaggaaggg gaaaaaatga gatttattca tacatcagat tggcatctag ggaagtccct
      421 agaaggccat tctagaattg aggaacaagc aaagttttgt gaggaattta taaaaatagt
      481 agaatcaaat gaaatagata tggttataat tgctggagat gtatatgata cttctaatcc
      541 cccagcacaa gcagaaaaac tattttatca gactgtatca agacttgcca ataatggtca
      601 gaggtgtgta ttaataattt caggaaatca tgataatcca gagagacttt ctgcaataac
      661 acctctagca catgaacagg ggatacttat atatggttat ccattaagtg ctacgataga
      721 agctaagtat aaagggtttg aaataacata tgcaactcag ggatgtacaa aactgaatat
      781 aaatggagaa aatattgtga ttgctacatt accttatcca agtgaaaaaa gattaaatga
      841 agttttttca agtgaagatg agtttgagaa acaaaaaaat tattctgaaa aggtaggaga
      901 tatatttaga agtttagaag aaaattttag aagtgatact ataaatatag ccgtatcaca
      961 tatatttgta ataggtggag agagtactga atcagaaaga cctatacaat taggtggaag
     1021 ttttttagtt gaaagaaaag atttgccaga aaaggctcaa tatactgcac taggtcattt
     1081 acataaacaa caaaaagctt ccgaacgttt aaatgcatac tattctggtt ctccacttca
     1141 atatagcaag gatgaaagag cgtataccaa aggggcatac atagtggata taaaagctgg
     1201 agaaaaacca ataattgaag atgtatattt taataattac aaacctatag aagtatttaa
     1261 atgtaatgga atagaagaag cactagatat atgtgagcaa aatcaagata gagagatttg
     1321 gtcatatttt gaaataaata ctgatgagat aatatcacag aatgaaatta aaaaaatgaa
     1381 ggaactatta aaagacataa tagaaataaa accaataata acatcttgtt atgaacaaga
     1441 aagtgtagat ataaaagaaa agtctatggc agagttgttt agagagttct attcttttag
     1501 taaaggtgta gagccaaaag gagaacttat ggatttattt ctagatataa taagtgaaga
     1561 aggtgaatca gcagatgaga ccaattagat tagaacttac aggacttaat agctatattg
     1621 ataaacaagt tatagacttt gaaaaattaa ttgaaagagg tttgtttggg atatttggaa
     1681 ctactggaag tggaaaatct acaatcttag atgcaataac tattgctatg tatggaaata
     1741 tatccagaaa tacaaaagaa tatataaaca gtgtgtgcga taaggcaatt atatcatacg
     1801 agtttgagat aggaagcaaa aacactagaa gaagatacat tgtagataga accattgcta
     1861 gaagcaaaac aggaacaaag acctctaatg ctagattaat agaagtattg aatgacaata
     1921 tccaaaatgt tttagctgat aaagttgtgg aagtaaatga aaaagtagca caggttgtag
     1981 gattaacggc aaatgatttt actaggtcag tagttttacc acaagacaaa tttaatgagt
     2041 ttctaagatt atcacgtgca gatagaagag acatgttaga gagaattttt aatcttgaga
     2101 aatatggaag aagtcttgga gaaaaagtga aaaagagaaa aaatatacag ttacaaaatt
     2161 taaaagattt gaaatcaaaa ttaagtcaat atgatggaat tacagaggaa gtatacaaca
     2221 atataaacca agaattaata gagttaaaaa atttggaaaa agataagaat aatgctttag
     2281 acctagctca aaaatcttat gaagaaagta aaacagtata tgaagaacaa ttaaagttag
     2341 aaaaaaatga acttagaaaa aatgagcttg atttaaaaaa tagtgagata aaagaaaaga
     2401 ttgctcttgt agaaaatgat agtaacgcta aaaaggtaga cccatacata agttcagttc
     2461 aaaatctaga aaaaaagata gatgaagata gctttgtagt aagtaattta gaaaagaaat
     2521 tagcaatatt aaatcaggag cttgaagtta caaaaaatag atatgaaaaa ataaacaaaa
     2581 ttaagaatga agaagtccct aaattgagtg aagaaaaaat aagattgcaa caagcaataa
     2641 aattagaaga agagttagtt ttattagata gagagttaaa agatctaaaa gaaagtggaa
     2701 ttaatttaaa taagactaaa gttgaattag aaaaagtaaa gcaggtgtca gagtccagaa
     2761 aagatgcagt tactaaatct ataaaagaag ttgaaggaaa gattgataaa gtaaatataa
     2821 gtgcagaatt aaaacaaaaa atatttctag catatgaata cgagaaagat tacaacaacg
     2881 tattggaaga aaaaaatcaa aagctaaata aactagaaga aatactcaaa gagacagaaa
     2941 atataaattt aaaagttaga tatattgata aagataagaa tgatgtaaat cgtaatttag
     3001 aaaatttatc acttcacttg gatgttcttc ttaaaaaatg tcctggtaag agtgctgatt
     3061 tacttttaaa atctgaatat gttactgaat taagaagtaa agcaaataat actaaagaaa
     3121 atgaaattaa gaaaagcagt atacaagatg aattaaagat aatacttgaa agtaagttta
     3181 atactgaaag ggaagtaaat ttactaaatg aaaaacttga aaataataga aagaatagag
     3241 atgacctaga aaaagaatta gaagaactaa agtatttaaa tttagcctct gaacttagac
     3301 gtgaattaaa agaaaatatg ccttgtccag tttgtgggtc aaaacatcat gaaaatcata
     3361 atataactaa gtatgatgaa aatatatctt ttgtcaaaga aaaactggag aaattagaaa
     3421 aagaaaaaat aagtataaga aacaatatag aggaattaaa tgctaaagta agtgggtatt
     3481 tatctataga aaaaatgaag actaaagaac ttgaagatgt aaaaggaaaa ctagggggga
     3541 ttccatctag tcaattatta aaaaaacttg atgaagaaca aagaaaactt gcattgttaa
     3601 aatctaatat tcaggagtgg gagaaagaaa aagagagtac tgaaaataaa gtaacattag
     3661 ctaaagaaga aaaaaataaa attgaaaaag aagaattaaa aataagagag agtctgaata
     3721 attataagaa attaacaaga gatttaaaca tagaaataga aggtttagaa aataaatgta
     3781 aaaaacttaa acaggaatac ctaggattaa agacgattac taaagtctct aatctattgt
     3841 caaaagtaaa agaaataagg gagaatgaaa aagaattaga attactaagt actgggcatt
     3901 caaatttatt aaaaaataga gactcactag atattaaaat aagagaaaat gagagtaaac
     3961 tacatgaaat agagatagaa ttaataaagg ctagagaatt atatgtagaa aagaaattga
     4021 gtagagataa taagtataaa gaagttatta acataacgaa aggtgattta gctaaaaatc
     4081 ttttacataa tgttgaagaa aatatatgta agatattaga acaagaagaa tcaagtaaga
     4141 aaaaattgga agagcaaaga ttagaatatg aaaaaaacat tgctgaaaag cacaatatag
     4201 atggtagact taaaactgct aaggaacaat ataaagacca aaaatcaaca ctaaataaac
     4261 tattagcaga aaataaattt gaaagtatat atgcagtaaa aagagcttta ttagatactg
     4321 atgcagtaaa aaaattgatt gaagaaatac tagagcatga agaagaacaa aagttattat
     4381 catttaagat aaaatcatca aaagaaaaat taaatggaag aagcataaag aaggagtact
     4441 ttgaacagtt aaaagatgaa atttataatt tgaaagtaga aataggaaat atatccaaag
     4501 atattggagc taatcaaaat cagttaataa cactaaaaga ctctttagat aagataaatg
     4561 attttaataa acaacttaag gtagttgaac acaatgtgga cttattggaa gaattagata
     4621 aatgtgtgca aggaaataaa tttgtagaat atgtttctac aaaccaatta aaatatatag
     4681 cattggaagc atcaaaacga ttggatggaa taacaaaagg aagatatgca cttgaaattg
     4741 actcaacact aaactttgta atgagagata actttaatgg aggagaaaga agaagcattg
     4801 atactctatc tggtggagag acattcttga catctctatc attagcactt gctctttcat
     4861 ctcaaataca attaaaggga agtgcaccat tagaattttt ctttttagat gaaggttttg
     4921 gttcattaga tagtgaactt ttagaaattg ttatagaatc cttggaaaga ttgcatagta
     4981 ataatctaag tgtgggtata ataagtcatg tcgaggaact taaaaataga gttcctgtta
     5041 agctattagt ttcatctagt gaggctggaa taggttcaaa agtaaaaatt gaatatagtt
     5101 agaaaatata tggtaattga atatattatc taatatttcc aaataatatg taattgtgat
     5161 ataataaaaa ctaatattaa agttatttat aattaatatt aatataaaaa catgtaagta
     5221 ggagtgtggt aagtgttgga atcgcccaat aatttgattc agattatttt tttaatagta
     5281 ttacttatag ggtctgcatt tttctcagca tctgaaacgg ccttaatgtc gttgagtaaa
     5341 atcagaatca gatatatgca agatgaagga gttaaaggag ctaagttagt aagttcatta
     5401 atagaaaatc caaataagct tttaagttct atattggttg gaaataacgt cgtaaatata
     5461 gcagcaacat ccatatcaac atctttgttt ataggattaa tgggagaaaa aggagtggca
     5521 cttgctacag ctgttatgac agtattagta ttaatatttg gtgagattac tccaaaaaca
     5581 atagcagcaa ataattcaga aaaggtttct ctattagtat ctaaacctat aaaagcaatt
     5641 atatttatat taagaccaat agtatggata tttaatataa ttacaaatat tatatttaaa
     5701 ttatttggta taacaaataa aggagctaaa tcattcataa ctgaagaaga gctaaaaact
     5761 atggtaaatg taagtcatga agaaggcgtc cttgaaatgg aagagagaga aatcataaat
     5821 aatgtttttg aatttggaga tatgcaagct aaaaatgcaa tggtacaaag aatagatatg
     5881 gtagctatag atatggaaga tagttatgat gagataatac aggtatttaa aactgaaaaa
     5941 ttaagtagaa tgcctgtgta tgaagaaact atagatgata ttgtaggaat acttaatata
     6001 aaagatatta tatttttatc tgatgaagaa atagagtcat ttgatattaa aaattatatg
     6061 agagaacctt ttttcacgta tgagttcaaa aagataacac aacttcttga agagatgaaa
     6121 cttgaaaaaa gtcagatggc tatagttgta gatgagtatg gtggaacatc tggactttta
     6181 actatagaag atttagtaga agtcattgtt ggagatatag aggatgaata tgatgaagaa
     6241 gaggatgaaa tacaagttat aaaagaagat gagtatattg tagatggaag tactaaaatt
     6301 ggtgatgtta atgaacttat tggtgtaaac ttggaatctg aggagtttga ttccatagga
     6361 ggttttataa ttggacacct aagcagattg ccagaagaaa atgaagtaat tgaagttgat
     6421 aatataagat tttgtataga aagtatagaa aaaaatagaa ttaaaaaaat aagaatatat
     6481 acataatgta ataatgttct taaatttatt agaagtgtat
//
LOCUS       DAEPOZ010000204         6449 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.204, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000204 DAEPOZ010000000
VERSION     DAEPOZ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6449
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.204"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(34..726)
                     /locus_tag="KR440_003026"
     CDS             complement(34..726)
                     /locus_tag="KR440_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBH0700036.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            complement(750..1625)
                     /locus_tag="KR440_003027"
     CDS             complement(750..1625)
                     /locus_tag="KR440_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HBH0700037.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFNILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIVLKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNSLGNTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            complement(1643..2764)
                     /locus_tag="KR440_003028"
     CDS             complement(1643..2764)
                     /locus_tag="KR440_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700038.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            complement(3177..4001)
                     /gene="spo0A"
                     /locus_tag="KR440_003029"
     CDS             complement(3177..4001)
                     /gene="spo0A"
                     /locus_tag="KR440_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HBH0700039.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            complement(4143..5201)
                     /locus_tag="KR440_003030"
     CDS             complement(4143..5201)
                     /locus_tag="KR440_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HBH0700040.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVGYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            5362..5802
                     /locus_tag="KR440_003031"
     CDS             5362..5802
                     /locus_tag="KR440_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0700041.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGCGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFKFNGELDYGGEKVMVLKSN"
     gene            5863..6354
                     /locus_tag="KR440_003032"
     CDS             5863..6354
                     /locus_tag="KR440_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0700042.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIVEAKKIGYTHMRLDTLPSMKSAQGLYEKFGFYDIELYTYNPIEGARYME
                     LKL"
BASE COUNT         2047 a         1115 c          678 g         2609 t
ORIGIN      
        1 atggtataaa gtatataatc tttcactaag atattacctc cattctttct ttttttgtcc
       61 taaaaccttt tttatgtgat aatactgttt ccctctatga ataaaacctt ttaaatttct
      121 tcctgtttca ttatgagtca tatttactaa tacttcttta attttaaatc catactttaa
      181 tatatcaatg gtcattccaa cttctactcc atatccaaaa ggtatttcat caaacttttc
      241 taaaacttct cttttaaata ttctttgacc tgataaagtt gcatctaact caacaccagt
      301 catctcaaac acagaatttt tagctaaacc ttttacaaat cccaaacctc cttttctctt
      361 tgcaggagga aacttggcta ttgtaacatc cgcctcacca tttaatatag gtactattaa
      421 tttttcaact tctttagcag agcttcctaa atctccatcc agaaaaccta taatatctgc
      481 atttttcatt gctaatttga taccataatt taaagcatat cctttacctc tatttttatc
      541 taacttaaaa aatttaatct tttcattttc tagctcagat acaaccttag cagtattgtc
      601 aattgaacca tcatccacaa ctattatttc atttatttgc ttaatgtcca atatgctttc
      661 taatgtatat tttattctac tttcttcatt ataagctggt attattatac ttatataaga
      721 atccattttt tctctcctta aaattatttc tatttaatag atggcaacaa tgaagttgca
      781 gtatccaatc taccaaaatt accaactata ttaccttgtt gtagaagaat agctagagat
      841 atacttccta taccttcatc aatattgtct atagttgcaa ctttgttttt agaatatagt
      901 tctacataag aagttttaac tcctgtattt tgaacttcta ctaaatattt attctctgat
      961 ttaagttttg aaactacaaa tttttctatt ttttcaaact gttttgtact gtcttttgca
     1021 tcatttccac cagctattac aacagaatca tattttagat atgtatttcc aagagagtta
     1081 aactttatta catctagttc ttgaagatat gttaaaatgt cacttgcatc ttccttttta
     1141 agggtgtcaa ttatataatt aactgcatca tttgcatttt ttacatctgt acctagttta
     1201 gttgacattt cctttaactt ttctggattt gttatatttt cttttaatac tatattaaat
     1261 gcaatacttc cattagattt atttattatg tcttctatat tttttgtata atcgtcattt
     1321 tcattagccg atattattcc tatactttta ccattcagcg aaccagctaa aatcttatct
     1381 acatttttat ttataaaatt aactgcctct tcataatctc tatttacact tgctaagtca
     1441 ctgtcaagtt tatcattttt ttcttttaag atattaaact tatcatccaa atcatttatt
     1501 atgtttgctt gttgttcact taattgttga ttattattta aattaaatcc tactaacatg
     1561 cctataccca aagctataaa tatagcccct atagtgacta tataatattt catattaata
     1621 tgcatattta tcctcctaaa atttacatct gtaataatac tctaaacttt aattgcatta
     1681 gttgtatgaa ctgttgaact cctggtgaca atgatgcaac taccaacaca ggaaataatg
     1741 ctgttgctat taatgcccat atgtacttta tttttaattt acttctgtat aacaaattaa
     1801 cgcctttagc atctataagc tttgaaccta tttttagtct aactaaaaaa gtacttgcca
     1861 taccttttct tcctttttca agaaaatcaa tcatatttga atgtgtacca actgctacta
     1921 ttaactcagc cttatactca taggcgataa gcatagctat atcttcacta gttcctggcg
     1981 caggaaatac aactgcatct aagcctaatt cctcaaccct cttaagacct ggtgctcttc
     2041 catctgtata agcatgtact actatttcac tagccttttt taaagcttca tcacttacac
     2101 tatccatatc acctacaatc acatctggag tatatccaaa ctcaattaaa gcgtctgctc
     2161 ctccatcaac accaaccaat actggcttta cctcttctat ataagaaagc atagtactta
     2221 aatcttcttt gtagtcctga cctcttacaa ctatcaatac atgtttatta gcataattag
     2281 ttttcacttt tggaatttct acttctccaa gtataaatcc tttttctttt ttagcataat
     2341 ctatagtatt atctataaat ctatccaatt ctacagctaa attctcatag gcagccttta
     2401 tttggtgtga aacctcatac ttgtctaaaa cttctcctgc acctaaaaat tttccttttc
     2461 tatatatctt tccatctaca acttctatag tttctccttc ttttaattct tcaaataact
     2521 cttctccaac attatcaatt attagtatat tattttcagt taatatccct ggacctttat
     2581 tgggatacct accacttata gaaggagccg catttatgac aagttttatt ttagcttcta
     2641 ctaaagagtt tgcagcaact tcatctatgt ctatatgatt tataactgct atttccccac
     2701 tttcaactct tttagcaagt tttttggttt ttcgatctac ttttataggg gcctcgactc
     2761 tcatatttaa acctccacta tcattattaa aatcacatta tattatagca ttacttaaca
     2821 ataaaataaa tgttttaagt ttgttttgca tctttggcat ttacaatact atatttcatc
     2881 atttttttat ttaacatatt ccataatacg cttaaaaata caaagtttac ctgcatcaaa
     2941 gtcaatattt atattaaaaa ataaaccact gtcagtttac aacttgtaaa gacacatact
     3001 atatccatag tataataaaa ttattatatt gatatagtaa gcaaataata aagaaaattt
     3061 ttatatttat taaaatagcg aaacggtata accctagtgg ttataccgtt tcgctgcttg
     3121 taatacgcat ttatagccaa tgccttaatt aaaaagcctt actttttatg tcttgtttat
     3181 ttaaccatac tatgttctag tcttaattta tcagcaatca ttgctataaa ttctgaatta
     3241 gttggttttc ctttagtatt gtgtaccgta tatccaaata attgatttat tgtgtcaact
     3301 tttcctctac tccatgcaac ctctattgca tgtcttattg ctctttcaac tctacttggt
     3361 gttgtattga attttttagc tatacttgga tataactctt ttgttactgc acctaaaagc
     3421 tcaacattat caataaccat ttttattgct tctcttaaat ataaatatcc ttttatatga
     3481 gctggtactc ctatttcatg tattatattt gtgatttctg tctctatatt tcctacattc
     3541 ttaacaaaat cacttctagt catttgagtc tcttgaactg gtctaggttt tggctcaact
     3601 tgtgtaactc tattagaaac tagctctcta attctgttta taaacacaac aaaatcaaat
     3661 ggctttacta tgtagtaatc tgctcctaga tttattgcgc tttgagttat cttatcttga
     3721 cctactgctg atagtactat tatttttggc attttaggta tatccatagt attcaatttt
     3781 tcaattacac ctaatccatc tagatgtggc attattacat ctagtattaa taagtccggt
     3841 tgtgtctttt ttactaagtc taatgcttca attccatcct tagctatgcc taatatatcg
     3901 atatcatctt cattagacaa atactctttt aatacctgac aaaaatcctt attgtcatct
     3961 gctaaaacta ttttgatttt ttccactaaa aatcccccca ttaaaaaaca tcttcttatt
     4021 acagaaaact aaacttttta cacaattgct tcctaattat ctactctaaa gcatattcga
     4081 cactccaaaa ttatattcct cctagataat tgataaaata ataaaatttt ttaatgcctc
     4141 acctacaatt ttatcatttc atctataaaa ataccatatc cttttttcgg attatctcta
     4201 aaaacatgag ttattgcacc aataatttta ttattttgta ttattggagc tccactcatc
     4261 ccctgtacga tacctcctgt gtattccaat aacttgtcat ctactacttt tattaccata
     4321 tctctataat ttcctttatc atatactata ctttctattt ttatatcata gcttgttata
     4381 tttttgtttt tatcctcaaa aagaattact gcatctccta tttttacatc ttgcggtctt
     4441 ccaacttcta ttaattgctt ttctttattt gctacattaa actctttatt atggttttct
     4501 gtaatattac cacttatacc tagctctgaa ttatttgaga aattacccat taaattgctg
     4561 tcattaaaat ctccttttat tttacctact ttttcattac tagcttttac tatttttaat
     4621 tgttctggct tatatatata gccttgcttt atctttaata gttcattagt atccgaatct
     4681 tttatggcat gaccgattgc tttgaacttt tccatactag ggtcgtaaaa agtcatagtt
     4741 ccaattccag atattttatc tcttacccat aatccaagtt tatacttgcc attttctttt
     4801 ttagtctcta ttacttctgt tttatactta ttttttctct caaaagtcac ttcaacttta
     4861 ctttttttaa tcttattcaa aatctctgat acatcttgac tattttttat ccttttatta
     4921 tctattttta ctatattatc acctatttgt atgcctccaa tatagcctac atcctcttcc
     4981 tcatatccta agaccaatac accatctgta tttgctttta ctccaattat atttcccata
     5041 ggatatacaa attttttatc atgattctta attgtaaaag cttctatttg agtctcttta
     5101 ttaacattac aatttttatt aaaaattata accatggtaa aaatcgatac agctcctaat
     5161 attatccaaa tatttttgaa atttttattt attttgttca tatatccatc tactcctatg
     5221 ctgtattatt atgtatttat aattcctttt tcctgagaat ttattcattc aaaaagttta
     5281 tttaaaatct taataagtga tttttaatac ataatatgat agtattagat tgtaacaaaa
     5341 agtttgaagg acggtaatat cttgaaatta aatgtaagaa ttgcagacat aaataattgg
     5401 cacgatttag ttagtttgtc tgtagataaa agtcagttag attacataga aagcaatgca
     5461 ctttctattg ctgaatctaa atttataaca gcttgggtac ctgttggaat atatgatgaa
     5521 aattctttaa taggatttgc tatgtatggt cgtttagaag atgatagagt ttggcttgat
     5581 agatttatga ttgattcaaa atatcaaggt aaaggatgtg gaaaagcatc attagatttt
     5641 cttgtaaatc atcttaaaaa tgaatataac tgtgatgaac tatatcttag tatattcgaa
     5701 gacaataaaa tggctattaa gctttataaa gattttggat ttaagtttaa tggagagctt
     5761 gactatggtg gtgaaaaagt aatggtatta aaatctaatt gatattaaga aaaatataca
     5821 tatatcaagg aggtgaagtc attatataaa catgtttata taatggcgga taaaatggct
     5881 caaaatataa ctttacaatt tgttgaagaa aaagatttag aaaatcttga aaacgtaaaa
     5941 cttctattta ctgaatattc aaattctcta aatatagact tatgttttca agactttaat
     6001 aatgaattaa aaacacttcc aggaaaatat aaaaaacctt caggctcatt gatattggca
     6061 tttgttgatg aaaatttggc aggttgtgtt gctttaaaaa aacttgaagg taaaatatgt
     6121 gaattaaaaa gattatatgt aagaaatcaa tttagaggat taaaaatagg aaaaatacta
     6181 ttggaagaaa tcattgtaga agctaaaaaa attggatata cacacatgag attagatact
     6241 cttccaagca tgaaaagtgc acaaggatta tatgaaaagt ttggttttta tgatatagaa
     6301 ctttatactt ataatccaat tgaaggggct agatatatgg aattaaaatt ataatttttt
     6361 aatagagtat aagtaaaact actacagttt tatttatact ctatttgtga acaaatctat
     6421 ttgaatattt atttaaatat gtatttatt
//
LOCUS       DAEPOZ010000205         6418 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.205, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000205 DAEPOZ010000000
VERSION     DAEPOZ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6418
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.205"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            143..946
                     /locus_tag="KR440_003033"
     CDS             143..946
                     /locus_tag="KR440_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBH0700043.1"
                     /translation="MMILPLSSVAIAKFYTEGLNNDMYEFYSLIILFFLIYSLLIVLK
                     LLNLINNNEFIIINLILTFISSLSIIIYSSNKKNLYPVKNIKLGSLLIIYFMISVIVP
                     TSFILILQGAQPNYSGIIKFIFTPITSLTTIYIFFSEEYAWRGFLQNIFFDKFGKKLG
                     VIILGMCWSLWHLPLIFTLYTPEAPILGVILRSIHIVGISIFLGYLYIKTKNIWFCAI
                     IHVLNNSAFLITNSKESTITYHDILIVSMVVLIFYVPFLFTKEYKNNLE"
     gene            complement(1045..2007)
                     /locus_tag="KR440_003034"
     CDS             complement(1045..2007)
                     /locus_tag="KR440_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBH0700044.1"
                     /translation="MNKSWSNLIIKNKDKKVRSGWIIITVMAAFYIFEYYLSTILMEI
                     IRKILIATGDINLKTNYFSEYANWLNDVGLQVVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVSWILV
                     FILVAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIII
                     GILFSYMFIKSSNIWMCIGYHFTWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSDKVNVTNIKN"
     gene            complement(2327..3277)
                     /gene="hpdA"
                     /locus_tag="KR440_003035"
     CDS             complement(2327..3277)
                     /gene="hpdA"
                     /locus_tag="KR440_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HBH0700045.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFKDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSAENISDIISFMHKNNLIEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDIDEEHLEELQDIFLDNGIACYVGHETAF"
     gene            complement(3270..3527)
                     /gene="hpdC"
                     /locus_tag="KR440_003036"
     CDS             complement(3270..3527)
                     /gene="hpdC"
                     /locus_tag="KR440_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HBH0700046.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDSACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            complement(3531..6239)
                     /gene="hpdB"
                     /locus_tag="KR440_003037"
     CDS             complement(3531..6239)
                     /gene="hpdB"
                     /locus_tag="KR440_003037"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HBH0700047.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDHRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
BASE COUNT         2167 a         1148 c          701 g         2402 t
ORIGIN      
        1 cccagtattt attaaaaatg aaataaaaag aaaaaaaata caaattatta tctttttctt
       61 tataaacttt ggtttaactt attcattagg tttactgtta tattataata aatttattga
      121 tccagaaaac tttgcatcat tcatgatgat attacctcta tcttcagttg ctattgcaaa
      181 gttttatact gaaggattaa ataatgatat gtatgaattt tattcactaa tcatattatt
      241 ttttcttatt tattcattat taattgttct taagttatta aatctaataa ataataatga
      301 atttataatt ataaacctaa tattaacttt tataagtagc ttatcaatta taatatattc
      361 atcaaataaa aaaaatttat atcctgtaaa aaatattaaa ctaggttctt tattaattat
      421 ttactttatg atttctgtaa ttgttccaac atcatttata ctaatactac aaggggctca
      481 acctaattat agtggtatca taaaatttat atttactcca ataacatcat taactactat
      541 ttatattttt tttagtgaag aatatgcctg gagaggtttt ttacagaata ttttttttga
      601 taaatttggt aaaaaattag gagtcataat attaggtatg tgttggagct tgtggcactt
      661 accactaata tttacattat atacacctga agcgccaata ttaggagtaa tattacgttc
      721 aatacatata gttggaattt caatattttt aggatatctt tatataaaaa ctaagaatat
      781 ctggttttgt gctataattc atgttttaaa taatagtgct tttcttatca caaattctaa
      841 agaatcaact atcacttacc atgatatact aatagtatct atggttgttt taatttttta
      901 tgttccattt ctatttacaa aagaatataa aaataacttg gagtaaatag aatatccaag
      961 ttatttttat atttttgtaa atttccaaca atcctacaga atactttaag tttagaagat
     1021 attattaaaa tttattataa tcaactaatt cttgatatta gtcacattta ctttatcact
     1081 aacaaaatca tacttagaat ctctatagta ataccataca aatataaagc taagtagatt
     1141 aacaaaagta gtcaaaattc caccttcaat accaaacatc cctccattta atagattacc
     1201 atgagtgatt tgtgtagtta taatccctgg aacctgaagt ccacttacag gcataccata
     1261 tataatcccc tgaaatacat tccatgtaaa atgataacct atacacatcc atatattcga
     1321 cgactttata aacatatatg aaaaaagaat cccaataatt ataatattaa ttacagacaa
     1381 aaaagtaaca tcaggattcc atatatgaat caacccaaaa ataacagatg gaacaatcat
     1441 tataaaataa atatttttac atcttcgtaa aactgccata aaaaaaccac gatttaaaat
     1501 ttcttctgca aaagcaacta aaataaagac aagtatccaa gaaactgtta aaggtgaaat
     1561 ttttggtacc catgaagtaa catgaccacc accaattgtt attgttataa taaagattac
     1621 tgtacaatta aatattccta ataacattcc tacatatcct tcttttttct taattctata
     1681 agaatgtaat cccatttcac taataggatt tttcataata aatctccata caattatagg
     1741 aataataatc ataagtgaat ctgttagaat ttgaaataca acctgtaacc caacgtcatt
     1801 taaccaatta gcatattccg aaaaatagtt tgtttttaaa ttaatgtcac ctgtagcaat
     1861 cagaattttt cttattattt ccataagtat agtgcttaaa taatactcaa atatgtaaaa
     1921 ggcagccata acagttataa taatccaccc tgaacgtact tttttgtctt tattcttaat
     1981 aatcaagtta ctccatgatt tgttcatata gatgtcttcc tcctaactat tatttttagt
     2041 tgtttctaaa atatattcta ttaaatttct tcatagtagt attttaccac aaaacttctg
     2101 cttcaataaa gtaaacgtaa tatattctct aactgtaatt taaactataa aaaatatata
     2161 aagtataaat ttaaagtaat atttatggac ataaagcaat atgaacaata tataaaacat
     2221 ataaaacttt acttaattat ttctacataa aaaccacata aaaaaggatg taactctagc
     2281 ttttagtgta acatcctttt ttaattaaaa aaacttgtat ttgattttaa aaagctgtct
     2341 catgaccaac atagcaagct ataccattat ccaaaaatat atcttgtaat tcttcaagat
     2401 gctcttcatc aatatctccc ttatcagaat actcatactc cttacctaat tgaatccact
     2461 ttgattctcc tagcctatga aatggtaata agttaatctc tattaaatta tttttgtgca
     2521 taaaagatat aatatcactt atattttcag cactatcatt aaacccagag ataactggta
     2581 ctctaagtac taaccttcca ttccagtcag aatttgcaag gtttgatata ttttctaata
     2641 ttaaatcatt atatacacct gtttgttctt tgtgtttttc tctatccata tgctttatat
     2701 caataaatgc aaaatcaata tctttaaata ttttattaaa aacttcattt gatacacatg
     2761 cacttgtttc aatggcagtg tgtatattta cctcatgaca ctttaataat acttcatgta
     2821 aaaattcatg ctgaagtagt ggctcacctc cactaaatgt tactcctcca ttacttctcc
     2881 aattgttaga atcacgttta attacctgca caagttcatc tacagtataa ggttttgcac
     2941 ataatttaaa cgcattataa taacatgagt tgacgcattc aaaactttca cagtccttac
     3001 agatgttcca atctataact ggcttattat caagattaaa gctcaatgca ccatttttac
     3061 acttaccatg acatacagta catccatttt catattgaca agataattca ctaaacatca
     3121 tatgtggtct tacagtccaa ctttctggat ttgcacacca tttacaactc agtggacatc
     3181 catttaaaaa tacagttgtt ctacaacctg gaccatcatg aactgaaaag ctttgtacat
     3241 cgaaaatcat gccttctaat tgcttttgac tactcattaa acacatgtcc ttcacaagta
     3301 attgcattca atgttgaata tacccagtct tctttctcta ggccaacaca tttgcctata
     3361 ccttcatcat tagcttcaac aaaattttta caatttttac attttggcat tacttttatc
     3421 tctggacatg ctgaatcatc taaattaatc atttgttttg ataatctaca aattcctttg
     3481 gttgcatcta ctgcacaaaa attcatacaa tcactatgct ttctcatatt ttacacccct
     3541 tcatactctg ttctagcaat tacttcatct tgtattggtt tacctaattc acaccaatat
     3601 tgagtaaacc cagcaacacg taccattaat tgacgataat tgtcaggatt tttttgtgca
     3661 tcttttaatg tttctgaatc tacaacatta tattgaatat ggaacccacc ttttcttaaa
     3721 taacttcttg ttaaatctaa taatttttta gttccttgtt gtccttttat agtagttgga
     3781 tgaagtttta aattcatctg agaattttga actactgctt ggtcccaaat agttgcagat
     3841 tcaaataatg catatactcc atttttatct gttcctgcat atgcagagac tgaaccatct
     3901 gaatatgttg ttccacttag tctaccatct ggagtagcca aggtagcagc tccctgtggt
     3961 ccatgtgtag aaacagatat ttggcaaggg tacatcttct tagcatataa agattgtgct
     4021 tcttcacata cttttgataa ccatacctca tagtttttaa gaatattatc tgcatacaaa
     4081 tcattatttc catatttagg tgcatcaaga caggccttgt aaatagcatc atatttacca
     4141 gtcttatcta cttttacttg gtctgccata gaataatttc ctacttctaa tgcatcttta
     4201 aatccaaaat tatttaatat tgcatccttc atttcctcta ttgtaaatgc tttgtcatca
     4261 tatacaagtt tcttaagtgc tgcaaatgaa ttgaccatag taacagtacc acaagtttct
     4321 acgtttaatg ttgcattata acgatatccc atatttccta tatgttgacc tttgtctaaa
     4381 cagtctggct ttagtagtga atttattata gaagtatcaa attttctcca tatatctaac
     4441 tcaatgttat ttgctctttc taaaacattt attgcttgtt tataataatc tttaaatact
     4501 tctatgactt cttcataagt atctaatggc ttattatgag gctcaaatac ttgtatattc
     4561 atacgatggt ctttaccatt cataagaaca agttctaaaa ttttgggatt agctataaag
     4621 tgaactccag agccagcaga ttgacctgct ccacctgcaa tagagtacgt ttttccgttt
     4681 aaagttaact gtttccaaca ccctggagac gtttccaaac atccacctaa tgcaaatgct
     4741 cttgcttctt caacagtcat accttcatca gcaaattgtt tcatcataaa tgttgttcca
     4801 tttgagttgt ttacccaggc tggataacca gaaccaagct ttgtacattc agcagctttc
     4861 attaagaaat cttctggaag cttttcatca tatagcatag ttaaactagg ttgaggagtt
     4921 ctacatctca tacttgcttc taataataac tcttctagtt cattagctcc tgtagaacca
     4981 tcttctttta atccacctat agataaagta ttaaatgtat tacctgatag tactcctcca
     5041 tttacgcccg ctgaagcaaa acagtcaata caagtgattt ttattctata aagctctaat
     5101 aattcgataa cctcttcttt tgtaattagt cctttttcta tatcttgttc ataccaagga
     5161 tataaaattt gaccaaaacg tcctatagac ataccagata tagcatcctc atttactgat
     5221 gctatatgaa ttgtataagt caactgaaga gcttccctaa atgtacgagg ttgtttatgt
     5281 gcaatatgtt ctaatatctc cactaaatct gagtattctt ttttagcttc taaatctgtc
     5341 tctatacttt ctaaatattt tgcatgtttt gcataattta aaatccaagt ttgtaatcct
     5401 tcaataactt gaataacagc ctcataaaaa tatagacggt ccataccaat aagcccatct
     5461 ccagatgcat ttcctgcaac tgccttttta cattcttttg ccatttctat aattccatct
     5521 aatccataat ttaaaggata aaaataattt ataacttctc taccttgagg aagtgtatac
     5581 cctgaatcaa acatgcatat aagtgtactc attaggtttt cttttaaatc ataatctggc
     5641 atcatttttt catagtgaaa acctaaatct tcaacagact ttccaaccca ttcctttgcc
     5701 atttttacaa gtacaggtac ttcttctttt ctcattccga atttaccagc tattgaaacg
     5761 acatttccaa aactttcagt aacatttcct ccaccagacc caaattttgt aagttcatca
     5821 gctgtgttac tggctaattt ctttgcctct tctctcattt gttctccttg agcaacaaag
     5881 aaactttcac ttacccatgg cattggaaaa gaacctctat aatggcgtgt cttttgcatt
     5941 actattttct caccaggtat tatatttggt gtcatatgtg aaaaagcagc tttaagagct
     6001 ttagctcttc taactactgg tatatcacca tctgattttc tatattctct attataccaa
     6061 tatggaaatt ccatatcagc agtagataat gtattataat atacatctaa taagtcttct
     6121 gctcttttac ttgctttttt ttcattaaat tcacttaaat tttctttatt ttgaaaattt
     6181 gatttaatat tttttgcttc caaaatactt cttattttgt cttctttact ttgactcatt
     6241 tcttcccctc cttaatcttt ctataattag tataaaactt tttaagaatg aaaaaaaagg
     6301 gtatattacc cactttttaa aataatcttt ctatagaaaa aattaaactt gaagcaaatc
     6361 taagccaaag acaatctgta tatcctaaaa tttgttgcaa ttcattggaa tataaaga
//
LOCUS       DAEPOZ010000206         6368 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.206, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000206 DAEPOZ010000000
VERSION     DAEPOZ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6368
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.206"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            263..1207
                     /locus_tag="KR440_003038"
     CDS             263..1207
                     /locus_tag="KR440_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HBH0700048.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1229..1855
                     /locus_tag="KR440_003039"
     CDS             1229..1855
                     /locus_tag="KR440_003039"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBH0700049.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1889..2938
                     /gene="hisC"
                     /locus_tag="KR440_003040"
     CDS             1889..2938
                     /gene="hisC"
                     /locus_tag="KR440_003040"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBH0700050.1"
                     /translation="MREKESIRELRGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYE
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            2941..3519
                     /gene="hisB"
                     /locus_tag="KR440_003041"
     CDS             2941..3519
                     /gene="hisB"
                     /locus_tag="KR440_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HBH0700051.1"
                     /translation="MRIWKVERNTFETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3521..4126
                     /gene="hisH"
                     /locus_tag="KR440_003042"
     CDS             3521..4126
                     /gene="hisH"
                     /locus_tag="KR440_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBH0700052.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDITLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELVAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4123..4845
                     /gene="hisA"
                     /locus_tag="KR440_003043"
     CDS             4123..4845
                     /gene="hisA"
                     /locus_tag="KR440_003043"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBH0700053.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGINTKIIEDIANNIDIPIQVGGGVRDKGKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSDVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4827..5585
                     /gene="hisF"
                     /locus_tag="KR440_003044"
     CDS             4827..5585
                     /gene="hisF"
                     /locus_tag="KR440_003044"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBH0700054.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKNMGVEVRL"
     gene            5613..6281
                     /locus_tag="KR440_003045"
     CDS             5613..6281
                     /locus_tag="KR440_003045"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBH0700055.1"
                     /translation="MDNKCNNVYSDEVEEFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDAIKKTLKDKVACYFSRSRQKLWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMEPEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2594 a          539 c         1263 g         1972 t
ORIGIN      
        1 gggaggaaat tatcatggtg agattaaata tagtaaaatt aaatacaaaa ttaaatcatc
       61 accatcatca tagattaaag ggattgtaat atgaaattaa ttcatagaca caagccctgt
      121 ttttagtgta attggtcttg tatctatgat gggaagtaat tttaggagaa aattatagcc
      181 atataggtat aaagcctata tggctttttt attgttttaa atttgcaaat ataagagtaa
      241 atttaatgaa gagggtgaga atgtgaaatt tttgaggatt gaagatgaag ttatttattc
      301 taaaaaaaga tataaattaa aaagggaaat agataaactt tttttggatg aagaatattt
      361 tcaaatagaa ccacaattat ttgaagaata tgatgaattt acaactataa ataataaaat
      421 tccagaagaa tctatggtaa aggttgtaaa tggaaaagta atggtactta gagctgatat
      481 aactacaagt ataataaaaa gccttgtacc aagatgggaa gatggtttaa agcttaagct
      541 tttttataat tcatcaatat ataaaaataa aaatacagtt ggaataaaag agataagaca
      601 aataggttgc gagtatctag gagaagcctc tgtagaagca gatagagaag tagtgaaatt
      661 agctcttaag atacttgaaa aatacaataa taattttatt ttagaggttg ggagtagcaa
      721 gtatattcat ggtttgttag aagaattaaa tttaaataag aattgtgaaa atcaaattaa
      781 aaatttacta tatacgaaga acactcatga attaaaagta tatattgaag aactgaaaat
      841 taagcgtgaa gtaaaagagc tattatcaaa tattttaagc ttgcaaggga atttatacaa
      901 cgttattgaa aaatcaaaca agttctattg taataataag atgaagcaag ctcttgaaga
      961 attaaagcaa gtaaataatt taattgagga atgtaatttt ttagataagg ctagatttga
     1021 tttatcaatg attactatgc ttgactatta tgaaggaatt atgtttagag gatactaccc
     1081 aaactcatat aaagaaattc ttagtggtgg aagatatgat tctttaacta aagagtatgg
     1141 aaaagaaatt ccagccattg gatttacact aagtgttgat gagttaatga agtatgtata
     1201 taagtaaagt ttagaaggag ggattgcttt ggattatttg actatagcac tagcaaaagg
     1261 acgaattgaa ggggaatcat ttaaaaaatt taaaaaaatg ggtctaggag atagtataga
     1321 cacagatacg agaaaactaa tttttaaaga tgaagaaaat aaaattatat atatacatgt
     1381 aaagccatca gatgtagtta catatgttga aaaaggagtc gctgaccttg gtatagcagg
     1441 taaagatact attttagaga atgagacaga tgtatatgaa atatatgatt taggttttgg
     1501 aaaatgtaaa tttgctgtag caggattaaa gggagatagc atatatagag aagatgaata
     1561 tctaaaagtg gcaacaaagt atcccaatat agcaaaaaag tattttaaag agaaaggtca
     1621 aaaaattgaa ataataaaat taaatggttc tgtagaacta gctccaatag tagggttgtc
     1681 agatgtaatt gtagatatag ttgagactgg caatacactt aaagcaaatg gtcttgaaat
     1741 attagaagac atatgtaata taagtgctag aattatatca aatagagcta gctatagatt
     1801 taaatatgaa caaattcaga atataattcg tttatttgaa gaacttgata attaaaatat
     1861 aattgaactt tataaggagg gatttaagtt gagagaaaaa gaaagtataa gagagttaag
     1921 gggatatgaa ccaaatcacg taaactgtaa agtaaaactt gatgcaaatg aaggaagtaa
     1981 gaggttgttt aaatacctta ttaaagaaat ttcagatagt gatatagatt taaatttata
     2041 tcctgaagat tcttatagtg atttgaaaga atctataata gattacataa atatttctgg
     2101 agtaaataag aaaaatcttc tagtaggaaa cggttcaagt gaaataattg atttaataat
     2161 acatactttt gtagataaag atgaagtaat attgagtttc tctccaagtt ttagtatgta
     2221 ttctatatat agtcagataa atgggtctaa atttataggt gttgaaagtg atgaaaattt
     2281 agttataaat atagataatg tgattgaaaa agtaaaggag aataatccta aaatagtaat
     2341 tgtatgtaat cctaataatc caactggaac aatattaaag agggaagaga taataaagct
     2401 tttagattca actaatagtc tagtggtttt agatgaagca tatatggatt ttggagaaga
     2461 aagcatgtta agtgatgttt ttaagtatga taatctaata gttcttagaa ctttatcaaa
     2521 ggcatttgga ttggctggaa taagaacagg atatatgtta tctaatagta gtttaataaa
     2581 ttctgttgag aaagtaagac ctccatataa tttaaattca ttatcagact ttattgctac
     2641 aagagcactt agaaataaag atgtggtaaa ggcttatata aaggaagtaa aagaggaaag
     2701 agaagtttta tatgaagaaa tgattggtat gggaattaaa gcatacaaat cacaagcaaa
     2761 ttttatatta ttttattctg aaatcgaaaa tttatcacaa aaacttattg atagaggagt
     2821 tttaataaga aaatttggtg gaaagttaga aaattattac agagttacaa ttggtgataa
     2881 agaagaaaat tctatgtttg ttggagctat aagagatatt ttgaaaaagg agaaataggt
     2941 atgagaattt ggaaggtaga aagaaatact tttgaaacac agattttagt agaattaaat
     3001 attgatggtt ctggtaaagc tgaaatagat actggaatag gatttttaga ccatatgctt
     3061 accttaatgt catttcatgg caagttcgat ttaaaagtga tttgtaaggg tgatacatat
     3121 gtagatgacc accatagtgt agaagatata ggtatagcta taggtgaagc atttaaaaat
     3181 gcactaggtg acaaaaaagg aatacgaaga tattctaata tctatatacc tatggatgaa
     3241 tctctatcaa tggtagcaat agatataagt aatagacctt atcttgtatt taatgctaaa
     3301 tttgataccc aaatgattgg tagtatgagt actcaatgct ttaaagaatt ttttagagct
     3361 tttgtaaatg aatcgagagt aacattacat atcaatctct tatatgggga aaatgaccat
     3421 cataagatag aaagtatttt taaagcattt gcaagagctt taaaagaggg tagtgaaata
     3481 gtttctaatg agatagcatc ttcaaagggg gttttgtaga atgaatataa tagtagacta
     3541 tggtcttgga aatatagatt ctgtgtctag gggttttaga aaagctggaa tagaaactaa
     3601 aatttctagt gatatagatg aaataaaaca ggcagattca cttatacttc caggtgttgg
     3661 agcttttaga gattctataa gtgccttaga taaactagga ttgataccaa taataaagga
     3721 gcatgtttct aagggtaaat ttatgatagg tatatgtcta ggaatgcaat tactttatga
     3781 aaaaagttat gagtatggag aatatgaagg attaggactt ataaaaggaa gtattgataa
     3841 actggatata actttaaaag tgccacatat gggatggaat aatctaaaat ttaataaggc
     3901 aaatgatgat atacttaaat atataaacga agatgattat gtatacttcg ttcactcata
     3961 ttatgcaaat tcatcaaatg aagaactagt tgcattttca gaatatgaaa agaaaatccc
     4021 tgctattgtt agaaaaggca atgtatatgg tatacaattt catcctgaaa aaagtggaga
     4081 agtagggtta aatatattaa gagcatatgg ggagatgata aaatgataat atttccagca
     4141 atagatataa aagataataa gtgtgtaaga ctcacacaag gagaatttga caaagtaaat
     4201 gtatattatg ataatccatt agaagtagca tataaatgga aaaatgaagg agcagaatat
     4261 atacatatag tagatttaaa tggtgctaga agtgaatttg gtataaatac aaaaataatt
     4321 gaggatatag caaacaatat agatatacct attcaagttg gtggtggagt tagagataag
     4381 ggaaaagtca aaagtttaat aaatgctggt gttactagag taatattagg tagtatagcg
     4441 attgaaaatt tgaatttggt tgaagaattg gtcaatgaat ataaagagaa aattgtagtt
     4501 tctatagacg ctaaagatgg aaaagtagca gttaggggat gggaagttgt aagcgatgta
     4561 gactcattaa cattatgtaa acaacttgaa aagataggtg tacaaactat tgtttataca
     4621 gatatatcaa aagatgggat gttgcaaggg ccaaattttg acatatatga aagaatagca
     4681 aaggaaacgt cattaaatgt tattgcttct ggaggcgtaa cttctataga agacgtaaaa
     4741 agactaaaag ctatgaactt atatggtgca ataataggta aagcgcttta tgataaaaag
     4801 atagacttta aggaggcaca acaattatgc ttactaggag aataatacct tgtctagatg
     4861 tgagaaatgg aagagttgta aagggaaaaa aatttaaaga tatagtagat gttgatagtc
     4921 cagaagtact tggaaaattt tatagtgatt gtggtgcaga tgaacttgta ttctatgata
     4981 taactgcatc aaatgaagag agaaagacat ctttagaatt tgtgacaaaa gttgcagaaa
     5041 atataaatat accattttgt gttggtggag gagtaaataa attagaagat ttcactgata
     5101 ttttaagaaa aggtgctgat aaggtttcta taaattcatc agcagtcaaa aatcctgaat
     5161 taataaggga agcttcatta aaatttggag cacaatgtgt tgttttatcg atagatgcaa
     5221 agaaaaatga agagggttca tggagcgtat acgtaaaagg tggaagagaa aaaactaatc
     5281 tggatgcaat tgaatgggct gttaaaggtg tagaacttgg agcaggagaa attgttgtaa
     5341 atagtatgga cgaagatgga atgaaaaatg gatatgatat agaattatta tcaaagataa
     5401 cttctttagt aaatgtacct gtcatagcat ctggaggcgc tggaaaaaag gaagattttt
     5461 atgaggcagt taataaatct aatgtagatg gaattttagc agcatctgtt tttcattttg
     5521 gcgagataaa aataaacgat ttaaagaaat atttaaaaaa catgggtgta gaagtaagat
     5581 tataagttaa atactaaaag gagattagaa atatggataa taagtgtaac aatgtataca
     5641 gtgatgaagt agaggaattt ataagaagta taaagtttga tgataaaggg ttagtccctg
     5701 ttgttgttca agaggtagtt agtaaagatg tactgatgtt agcatatatg aataaagatg
     5761 ctataaagaa aaccttaaaa gataaagtag cttgctattt tagtagaagt agacaaaaac
     5821 tttgggttaa aggtgagaca tctggtaata ctcaaaaagt agttaagatg tcttatgatt
     5881 gtgatgtaga tactatacta ctttttgttg aacaaactgg tgtagcttgt catacaggca
     5941 attattcttg tttttacaga gacttgtttg atgatagttc aaaaatggag cctgaagtac
     6001 aaacaaatat attgaaagaa ttatatgatt taatcaatga aagaaaaaat aatccagttg
     6061 aaggttctta tactaattat ctttttgaaa aaggaataga taagattctt aaaaaagtag
     6121 gtgaagagag tagtgaggta attatagcat caaaaaatac cgataaaagt gaacttattt
     6181 acgaaattag cgatttagtg tatcatacat tggttttaat gatagaaaaa ggtgttgaaa
     6241 ttgatgaaat aaaaaaagaa ttacttaaaa gaagaaaata gaataggata gattaatggt
     6301 aataagaaaa tataccgctt ttaataaaaa gtggtatatt ttttatttta tattttcgat
     6361 ttatagaa
//
LOCUS       DAEPOZ010000207         6307 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.207, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000207 DAEPOZ010000000
VERSION     DAEPOZ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.207"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            243..971
                     /locus_tag="KR440_003046"
     CDS             243..971
                     /locus_tag="KR440_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0700056.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            993..1910
                     /locus_tag="KR440_003047"
     CDS             993..1910
                     /locus_tag="KR440_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HBH0700057.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTEAGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            2052..3236
                     /locus_tag="KR440_003048"
     CDS             2052..3236
                     /locus_tag="KR440_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBH0700058.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPSNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTSLDVINFDKVESIVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGVDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            3343..3531
                     /locus_tag="KR440_003049"
     CDS             3343..3531
                     /locus_tag="KR440_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBH0700059.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            3681..4331
                     /locus_tag="KR440_003050"
     CDS             3681..4331
                     /locus_tag="KR440_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBH0700060.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENMKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEELSESEPTYIVENVESIKDILL"
     gene            4434..5372
                     /locus_tag="KR440_003051"
     CDS             4434..5372
                     /locus_tag="KR440_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBH0700061.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            5398..6249
                     /locus_tag="KR440_003052"
     CDS             5398..6249
                     /locus_tag="KR440_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBH0700062.1"
                     /translation="MNSKIKYFLKFFILIFILIFIYSVAIEPSLLIVKKYNISENGAL
                     IKNDTIESKSESVKVVQISDTQIGSFYSTKNLKKVANKINTLNPDIIVFTGDLIDYSN
                     KNPSVDEITTILSSMNARLGKFSVFGNHDYMYKLPRYYRQIMKNSNFNLLVNENKKIK
                     LKDDKYINILGVDEILNGNPNIKYLESQIDNKNFNLLLAHEPDLVDMLSKDTMNLVLS
                     GHSHGGQIRLPIKGALVTPPYGRKYTKGFYDINGNHLYVSSGLGSTKLPFRFFNIPEI
                     IEFNIII"
BASE COUNT         2579 a          613 c         1131 g         1984 t
ORIGIN      
        1 tgttaaaaga taatcaaaaa ataatattag agtatataga aggttaaatt ttaaatacat
       61 ataagttgat atactaaatt tctgagatga gtttacataa aactcatctt ttttactttt
      121 caataacttt gttttattat ttaatataca ttttatctgc ttagagttgc tgatatataa
      181 aaattactga tataataaaa gaatggatat taatgtaata ttttatagaa aagaggggta
      241 ttatgcttga aataaaaaat ctttccttta atgttgagtc taacaatgag gaactaggaa
      301 taataaatga tgttagcctt tcatttgaga ggggaaaact tattgttatc acaggtccaa
      361 atggtggagg aaaatctaca attgctaagc ttattatggg aattgaaaaa gctacgagtg
      421 gtcagataat tttagatgga gaagatataa caaatcttag tattacagaa agagcaaaaa
      481 aaggaatagg atacgcattt caacaaccac caagaataaa aggtatgaca gttgaaaatt
      541 tacttacact tgctcatgga aaaccattgt ctactgatgt atgttgccaa taccttacag
      601 atgttggatt gtgttcaaaa gactatttaa atcgtgaagt tgataactct ctttctggag
      661 gggaaatgaa aagaattgag atagcaactc tttttgctag agatttaaag gtttctatat
      721 ttgatgaacc tgaagcaggg atagaccttt ggagctttgg aaaactcaat gaaagtttta
      781 agaaaataca tgaagagtct aatcagacta taatcataat ttctcaccaa gaaagaatat
      841 tagagttagc tgatgaaata attgttctac aagatggttc agtaaaaagc catggaacaa
      901 aagaggctat attacctgaa atcatgtgtc aagttaattc aagttgtgaa ttaatgaaag
      961 acatgaatta attagaaagg aaggcaaatt aaatgaacaa aatagattta aatcttttag
     1021 aaaaagttgc tgaacttcgt ggaactccat taggagcata caatatacgt aaaaatgggc
     1081 aaggagtaga gagacattca actgaaaatg taactataat tcctaaaact gataaacctg
     1141 gaatagatat tatagtaaag ccaaatacaa aaggagaaaa tgtacatata ccagtaatag
     1201 taacagaggc tggtgtacag gatatggtat ataatgattt tgaaattgga gaaaatgcag
     1261 atgttgttat aattgctggg tgtggtatac ataactgtgg ttgtgatgat agtgaacata
     1321 atggtataca tagatttttc ttacgtaaag gagctcgcct aaaatatgta gaaaaacatt
     1381 atggtgaagg tgatggagaa ggaaagagaa ttttaaatcc agttactatt gtcaatatgg
     1441 atgaggattc atattgtgaa atggaaacaa ttcaaattaa gggtgtagat tcaactaaaa
     1501 gagaaatgga tgcaaaccta ggtaaaggag ctaaaatagt tgtatttgag aaattattaa
     1561 ctcatggtaa tcaagttgca gaatctaata tgaaggttat cttagagggt gaaaactctt
     1621 ctgctcaagt agtttctcgt acagttgggc aagataattc taagcaaaag tttaatcctt
     1681 gtgttattgg ggattccctt tgttctgcac atgttcaatg tgattcaata attatgggaa
     1741 attctcaaat tagtgctata cctgaaattg ctgcaaatca tccagatgct cttttagtac
     1801 atgaagcagc tattggaaag attgcaggag accaaatact aaagatgatg acattaggtc
     1861 ttactgaaga agaagccgag caacaaatac ttaattgctt cttaaagtaa attaaagatt
     1921 ttatttaatt taatataatt aatatagtat aatatagtga ataagtctaa aatattttta
     1981 tttttaggct tattttcttt atgtaagaaa agctattaga gaaattatat tgtaattagg
     2041 atgtggaata aatgaaaaat ttacttataa gaaatgggac aataatatct ccaacagatg
     2101 gtatcaaatt tgaaaaaaga gatatcttgg ttttacatgg gaaaatatat gctattggat
     2161 tgaaccttaa agagaaaata agaaaagaaa aattacttac aaataaagag ttcgagataa
     2221 taaatgccga taatatgtat atttccccag gatttataga tgttcacacc cattgttata
     2281 agagaaaatt acctacaggt atggattctg attcaattgg aattgaaaaa ggttctacag
     2341 ttatatttga tgcaggtaca gctggtccct caaactacta tgattttaaa aaaaagtata
     2401 tggatgagtg taaaacagaa gtatattctt ttttaaatgt tacaaatgag ggactaaata
     2461 tattgaatga atcaacttct ttagatgtta taaattttga caaggttgag tcaattgtag
     2521 aaaaaaatat agaaaagata aagggtataa aagtaaaagc gagtaaattt caatctaatg
     2581 aaagtggtat agatataatt aagaaatcaa agcaagttgc taaaaaatta aatttaccct
     2641 tagtggtata tataggagag tatccatcat atatagatga agtaatgaat attttgggaa
     2701 aaggagatgt ggttacacca gtatatggaa atagtacaaa tggaatcctt aaagaatctg
     2761 gcacattaag aaaatcagta ataaatgcaa agaatagagg agtattgttc gatgtatgtc
     2821 atggagtgga ccaatttgat tttaaagttt ttaaaaaagc aataaagcaa ggtttagagc
     2881 ctgacctaat aagtactgat atgcatataa aaaacatgaa agacgtaaac tattctcttt
     2941 taaatgttat aaataaaatt atggaacttg ggatatccct agaaaagtgt atagaaaaag
     3001 taactgaata cccaacaaag attttcgatt tacatggaaa taaaggcaga ataaagattg
     3061 gaggagaagg tgattttaca atattcacaa tggaggagtg taatgatatt ataaaagact
     3121 acaataataa tgaacttgtt ttaaataaaa aattaagatt gcaatatact gtaaaatcat
     3181 ggatgaaaag cagtgaagtt tatagacatg gatacgaaaa tactataata aactaaacaa
     3241 tagtgataaa ataataaata tatgtttaaa ttggtatata ttggataaat atctaaggaa
     3301 acaacaatta aaaaataata attttagggg aggtatttta gtatgaaagc atttgttgat
     3361 aaggatgtat gtgtagcttg tggagcatgt ataggaacat gtccagacgt ttttagtatg
     3421 agcgatgaag ggcattcagt agcaattgaa ggagaaatac cagcagattt acaagattta
     3481 tgtaaagaag ccgaagaagg atgtccagtt tcagctataa cagttaaata gagctgttta
     3541 aatttatttt taaaataaat atataagatt taattgggta cttaagctat agtattagaa
     3601 atttctatta ctatagcttt ttaaaataga taaacttaat atgtaagata ttacttttta
     3661 caaatatgga ggactgaaat atgaaaaaaa attatgaaat agtattattc gatttagatg
     3721 gaacacttac agaccctaaa gaaggaataa caaagtctat tcagtattct ttaaatagct
     3781 ttggcataaa agaagatttg gaaaacttag accaatttat aggaccacca ttacatgata
     3841 ctttcaagga gtattataaa tttgaagata aaaaagctaa agaagctgtt gaaaagtata
     3901 gagaatattt tgctgataaa gggatatttg aaaataaaat ttatgagaat atgaaagaga
     3961 tattagaaat gctctataaa aatggtaaaa tattattagt agcaacttct aagccaacag
     4021 tttttgcaga aactatactt agatattttg atatagatag atattttaaa tatatagcag
     4081 gtagcaatct tgatggtaca agagtgaata aaaatgaagt tatacaatat gtactagacc
     4141 tatgtaatgt aaaagataaa gataaagtta ttatggttgg agatagaaag tatgacataa
     4201 ttggagctaa aaaaataggt atagattcta ttggagtatt gtatgggtat ggttcttttg
     4261 aagagcttag tgaaagtgaa cctacttata ttgtcgaaaa tgtagagtct ataaaagata
     4321 ttttgttata aatatctttt atagacaaaa atattacaaa atggtgtcgc atatttaaaa
     4381 atatgatatt atagttaagg aagtcattta aaaaagagga ggatgcactt aaaatgaaat
     4441 ctattttaat tatgggtgga agcgacttca taggttctgc acttgctaag cgcttgatta
     4501 aatgtgggta tcaaattgat atacttacaa atggtaaaaa ggaaatagat tataatggtt
     4561 ttaaagaaca tctaatttgt gatagaaaag ttcgaaaaga tatggaaaac attatcacag
     4621 gaagaaaata tgattacata tatgatatga cagcatatac aaaagaagat gtaagtaatc
     4681 tgattgattt tataagcatg gataacttaa aaaagtatat agtattgtca gcaggagcag
     4741 tatataaaga tagtggaaga aatataaaag aagaaaatga gaaaggtgaa aatgaaaact
     4801 ggggaaaata tgggttaaat aaaaaagagg ctgaagattt tattataaat agccctatac
     4861 catatataat tattagacca acttacatat atggtgaaaa taataattta tatagagagt
     4921 attatttctt tgaaaaaata gaaaaaaatg aaaagatacc agtaccaaaa ggaaaacaag
     4981 ttagtaatca atttatatat attggggatt tggtaaaggt attggaaagc ataatgaaaa
     5041 atcctcatgt aagagaggcc tataatgtga caaatccaca gttaatttct tgggatgacc
     5101 ttatatatac atgtggagaa attattggca aagaacctat aataaaatat gtagatatgg
     5161 aaaaagtaga gtttagagaa agaacgtatt tcccatttag aaatatagac tttaatcttg
     5221 atataaataa gcttattgaa catgggcttt atataccaaa tgtgttacta aaagaaggtc
     5281 ttacagcaac atataaatgg ttttcagcaa ataagccaaa aatgcatgac agaaaaatga
     5341 acaaagttga gtctgtaatg caaatagttt aaaaaaataa ataagtaggt agatattttg
     5401 aactcaaaaa ttaagtattt tttaaaattt tttatcttaa tatttatttt gatttttata
     5461 tactcagttg cgatagagcc gagtctatta attgtgaaaa aatataatat aagtgaaaat
     5521 ggagcattga taaaaaatga tacaattgag agtaaatctg aaagtgtaaa agtagttcaa
     5581 atttcagata ctcaaatagg tagtttttat tcaactaaga atctcaaaaa agtagctaat
     5641 aagattaaca ctttgaatcc agatataata gtatttactg gtgatttaat tgattactcc
     5701 aataaaaatc caagtgtaga tgaaattaca actattttat caagtatgaa tgctaggttg
     5761 ggaaaatttt cagtatttgg caatcatgat tatatgtata aacttcctag gtattatagg
     5821 cagattatga aaaattccaa cttcaatctt ttagtaaatg aaaataaaaa aataaaatta
     5881 aaagatgata aatatattaa tatattaggt gttgatgaaa tattaaatgg aaatcctaat
     5941 ataaaatatc tggagagtca gatagataat aaaaatttta atttattgtt agcacatgaa
     6001 ccagatttag tagatatgct tagtaaagat acaatgaatt tagttctatc tgggcatagt
     6061 catggtggtc aaataagatt acctattaag ggagcattag ttactcctcc atatgggagg
     6121 aaatatacta aagggtttta tgatataaat ggaaatcatt tatatgtgag ttcgggtctt
     6181 ggaagtacaa agttaccatt tagatttttt aatataccag agataataga atttaatatt
     6241 ataatctaac ataaaatatt tagaaaatat aattttgctt tttataatat gtagatgtat
     6301 atactgt
//
LOCUS       DAEPOZ010000208         6297 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.208, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000208 DAEPOZ010000000
VERSION     DAEPOZ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6297
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.208"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            173..2260
                     /locus_tag="KR440_003053"
     CDS             173..2260
                     /locus_tag="KR440_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBH0700063.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMEDISINHFINSIKVSRSTVNLDFKDLIPELEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLIEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPYNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIYLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            2275..2562
                     /locus_tag="KR440_003054"
     CDS             2275..2562
                     /locus_tag="KR440_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0700064.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            2589..3860
                     /locus_tag="KR440_003055"
     CDS             2589..3860
                     /locus_tag="KR440_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HBH0700065.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            3918..4517
                     /locus_tag="KR440_003056"
     CDS             3918..4517
                     /locus_tag="KR440_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBH0700066.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIVLQSLSVVLQYAKGLNTQQYLKWHPAGSLGKSIREGI"
     gene            4522..5427
                     /gene="dapA"
                     /locus_tag="KR440_003057"
     CDS             4522..5427
                     /gene="dapA"
                     /locus_tag="KR440_003057"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBH0700067.1"
                     /translation="MKYKGIICAMITPFDEKQNINSQATCKLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVIDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEVTQNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKAQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            5533..6165
                     /locus_tag="KR440_003058"
     CDS             5533..6165
                     /locus_tag="KR440_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HBH0700068.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGEILCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGINLENLHIWLENGVDVCGFGGLLTKGTIEDITENAKKIREIIKNIREI"
BASE COUNT         2328 a          750 c         1167 g         2052 t
ORIGIN      
        1 gtaatatagt agctatttta gataagttta atttttgtct caatctaaaa caaaagttat
       61 acttaaatga aattgaaaac gataaaattc tcaaataaaa taagtattgt ggaggtgatg
      121 aaagaatcca atgataatat gaagactaat cttaataaga aagtaggggt taatggtagt
      181 ggacaaagaa attgtatcaa ttctccagat tttattaaat agcacattga ttacaactaa
      241 tggattacaa gaggagtcta attcatcaaa gagacaaatt acttatagaa taaataaaat
      301 caatgacatg cttaaagtaa aaaatgtacc acttatatgt ttaagagctg ataaagatat
      361 tatcgttaga aaagaaacaa aaaaagcaat taaagaaatt ttagaaaaaa actattctaa
      421 gaatacttat tattttagta agacagaaag acttttatat atgtatttaa tgctttttat
      481 taacatggag gatatttcaa ttaatcattt tataaattct ataaaagtaa gtaggtctac
      541 tgtaaatctt gatttcaagg atttgatacc agagcttgaa aagaagaaca ttaaagttaa
      601 gaacaatcgt ataaatggat attacatagt tggcaatgag atggaaatta ggagagtgct
      661 tattaaaaac attatagaaa ctttgtctaa tgagaaaagt tcaagagtat ttgatatttt
      721 tattaaagaa tataatttag atagttttga agaggctaaa aaaattatat taaagctaat
      781 aaaaaaatat gaaattactt ttgttgaaga tagattaata gaatttatat atatatttat
      841 ctttctaaaa gcaaggatgt attcaactag aatagttact caggaaaatg tagatattcc
      901 taatatttca gttatgagtt caatgaagga gtataaattt gctaaggaat tattagaagt
      961 atatgggagt gaggataaga taaagcctta taatataatg tatattagtg cttggatttt
     1021 aggtatatcg gtaggaaatt atgaagaaga tacagaagat aaggatgtaa tttcaaaaat
     1081 tgtaaataaa atgatgacaa agtttggata tttaagcgga gtttactata taagccaaga
     1141 aaaaatattt aaacaattat attctcattt tagacctgcc tactatagac ttttatttaa
     1201 attacctatc tataatccga tttgtgaaaa agtaaaagaa gaatataaat tagtttatag
     1261 aattgtaagt aatgcaatga aagagttttg cggattattt ggagaagaaa ttcaggaaga
     1321 agagttagca tatcttacaa tgcactttgc aacacttttt tcaaataaaa aagaatttga
     1381 tggttttaga aaaagaatag cattaatagt ttgttcaaat ggcgttggta gttcagctat
     1441 tttacatgca gaacttacaa acttatttcc tgacttacac tttataccaa tgtcggaatc
     1501 agttggatta gaaaatataa gtgaacctgt agatatcgta tttactacta attataacgc
     1561 agagggttta aaaacagatg taccagtaat taaagtaagc cctgttatga ccacaaaaga
     1621 aaaatataag attacacgtg aagtgtatat tcaattaggt gatatttatt taagacaacc
     1681 taaaatagat gaggttatgg atataataaa aaaatatgca aatgtaacct ctgaaagtat
     1741 tctttcaagt gagctattag cttattttac acagatagag aattttacat ctaaggaagg
     1801 agaggggcct atgctaagtg agattactaa tgaaacacta gtaaaaataa aggtaaaagc
     1861 taagaattgg gaggaagcta ttagacagtc agcttctgta ttagttgaaa ataacaaagt
     1921 aactgaaggg tatgtagatg ctatgattaa ctcagcaaaa gcatctggac catacatagt
     1981 aattacaaaa catgttgcac ttccacatgc tagaccagaa gctgggtcaa aagagattgc
     2041 aataggcatt gctactttag atagtcctat tgagtttggg aataacgaaa acgaccctgt
     2101 aaaatatgta ttttgtctaa gtgcaataga caataatagc catttacgtg ctatgtctga
     2161 gctagttgaa ctattggaag aagatgagtt ttttaaggtt ttagatagtg ctaaagaggc
     2221 taaagaaatt attgatttta taaaaacaca cgaattataa aggaggattt aattatgtat
     2281 aaaattttaa tcgcatgtag agcaggagtt ggctcaagtt taatgttaaa aataaagact
     2341 caacaaatta ttaaggaaaa taactttcca attgaagtag aacatggttc attggattca
     2401 ctaaatggat tcaatggaga tgctgtagaa acattaattg atgttgctga agaattaaaa
     2461 aataaaaatt tgaagtttga tataattggg attcacaata ttgttgaccg taatgaaata
     2521 aagacagcac tagaaaaatt ccttgcttca aaagagggat aaatttaata tgataaggag
     2581 gaatatttat ggagtttatt attagtttgc tgagtacacc tgctgttttg cttggcgtag
     2641 ttgcatgtat aggattgatt cttcaaaaga aatcagccgt tgaagtattt acagggtctt
     2701 caaaaacact tatcggtttt ttgatatttg gtattggtgc ctctgctatg acaggagcac
     2761 ttcaaaattt caataaactg ttccaacatg gctttggaat tactggtgtt atagcttcac
     2821 cagaagcagc tacagcactt gcacaaggta catatggttt tgcagtttca tgtacattaa
     2881 ttcttggatt catattaaat ttagtatttg cacgtattac aaagtttaag aatattttct
     2941 ttacaacagg tcatagttta ttcttttctt gtgtattagt tttaatcatg aaagcacatg
     3001 gatttgataa tacaatcact attttaatag gtggaactat cttagggttt atgtctgctg
     3061 cactacctca attctgtcaa ccatttatgc gtgaattaac aggtggcgac gaacaagcta
     3121 ttggtcactt taacatgatt ggttatggtt tatcaggtta tattgggcgt ttattttcaa
     3181 aacataaaga tgatacaaca gagactatag aatttcctaa gtggttatca atattcagag
     3241 acttcttaat gggtctatcc atagttatgt taatcttatt ctatattgca acattaaaag
     3301 ctggtcaagc atttactcaa gaaattgctg gttcaacaca ttggttggtt tttccgctta
     3361 tagaggcatt tacatttgtg gcaggtatgt caatattgat gtcaggtgtt cgtatgttct
     3421 tagctgaaat aactgcagca tttgtaatca tttcagaaaa gtatattcca ggttcacgtc
     3481 ctgcgctaga tgttccaaca gtatttccat atgcaccaaa tgcagttatt attgggttta
     3541 tatcagcata tgctgctggt ctattagcaa ttgctattat ggcatcattt cctagtatat
     3601 ttcctgtagt aattattcca gctgctcaca tatgtttctt ctcaggaggt acagctgcaa
     3661 tatttggcaa tacttcagga ggatggagag gggctattgc tgggtcattt gttgttgggt
     3721 tattacttgc atttttacca gttatactat atccagttta tgggactctt ggtatagaag
     3781 gagcaacttt ccctaatatt gattacaacg tagttggttc gatattacat aatattttac
     3841 aatttataaa acctatttaa actaaattat ataaaagtat agtcatagtt actatgattt
     3901 caaggaggat acatcttatg gaaaatacat tatctgaatt tttagataat atatctaatg
     3961 aattgtcaga atttattgcc tcaatagatg agacatctat aaataaagca tgcgaattaa
     4021 ttcttgaagc tgaaaaaaat cacggacgtg tacatgtgac tggtattggt aaaccaggac
     4081 atgtatcagg atatatttca tcattgcttt catctactgg aacatcagca tatatattgc
     4141 atggtacaga agcagtacat gggtcaagtg gtcaagtagt tgaaggagat gttgttattg
     4201 caatatcaaa tagtggtgaa actcaagagt taaaagcaac tcttaagact ctaaaagtaa
     4261 atggagcaaa aataattggt gtaagtggta atgagagttc atttttaaaa aatatttctg
     4321 atatattcct gttcgcaggc gttaaacagg aaggagattg tcttaataaa gctcctcgtg
     4381 cttcaatttt agctgaaaca atagtattac aatcattaag tgttgtatta caatatgcta
     4441 aaggtcttaa tacacagcag tatttgaaat ggcatccagc aggaagtctt ggaaagtcta
     4501 ttagagaggg aatataattt tatgaaatat aaagggatta tatgtgcaat gataacacca
     4561 tttgatgaaa agcaaaatat taattcacaa gcaacatgta aattaattga ttatttgatt
     4621 gaaaagggga tttatggatt atttattttg ggtactaatg gtgaatgtca tgtgttaaca
     4681 gatgatgaaa aagttgaatt tgcaaagatt gttattaatc atacaaataa tcgtgtacct
     4741 gtttttgttg gaacaggtgg taattcaact cgtgaagtta tagatttatc caagagaatg
     4801 gaagcgattg gagcaagtgc attatctatt attacaccat actttgttac accaacacaa
     4861 caggaattaa tcttacatta caaaactatt gctaattcag ttaatttacc tattataatg
     4921 tataatatgc caggtaagac tggaattaat attgaaccag agtcagtacg tgaattatca
     4981 aagataaaga atattgttgg aattaaggat agtagtggta aacttgacaa catgaaagct
     5041 tatattgaag taacgcagaa tgagaatttt agtgtgtttt caggctcaga ttcacttatt
     5101 ctggatagtc taaaagctgg tagtcaagga gctgttgcag caacaacaaa cttcctcaca
     5161 gagattggta tttctatcta tgaaaacttt atgaaagggg atttggaaga agctcaaaaa
     5221 gctcaaagta gtatagaaga acttcgacgt atactaaaac ttggaacaat tccatctgtt
     5281 atgaagaaaa ctattgtctt gaatggaatt aatgttggaa ctgcacgact tcctgtaact
     5341 gaaccaactg gggaagtact tgaagaaatt aaacgagtag ttgaaaatta taggacaata
     5401 cttaataaaa gtagtgagaa tagataatat aaatagggaa attacactgg tatataatta
     5461 atatatatat tataaaacat gactaaaatt aatgaataag taaagtggtt tgatgagtat
     5521 agaggtgata atatgaaaaa gatgaatata accaaaagaa tgtcagaatg tggggctctt
     5581 gcaattgtaa gagaagaaaa tcttaaccgt gcatgtgaaa ttgctgaagg atgtataaaa
     5641 ggtggtataa cagttataga aatgagttat accctaaata atgctggaga aataattcaa
     5701 gggcttaata aaaagtatgg tgaaatactt tgtgtgggag caggaacagt tttggatagt
     5761 gaaacagctc gtcatgctat attacatgga gctcaattta ttattgcacc aaattacaat
     5821 gaaggtgtag cgaagatttg caatagatat cagattcctt atgctccagg atgtacatca
     5881 ttgactgagg cagtagatgc actaagttta ggagcgactt ttataaaagc tttcccaata
     5941 tcagatttct atggacctaa actagtaaag gtatttaaga caccaatacc tgatatgcca
     6001 gttttagcaa gtggaggaat taatcttgaa aacctacata tatggttaga aaatggagtt
     6061 gatgtatgtg gatttggtgg tttattaact aaaggaacta ttgaggatat tactgaaaat
     6121 gccaaaaaga ttcgtgaaat cattaaaaat ataagagaaa tttaacatgg aggatgaatt
     6181 agttttctat tgacgagata tccacacatt aattcctaag ttagaagaaa tattttctag
     6241 ttgaggtaaa gtttatattg aacttaaatt aaattttgtt ttaaataaga tattatt
//
LOCUS       DAEPOZ010000209         6274 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.209, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000209 DAEPOZ010000000
VERSION     DAEPOZ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6274
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.209"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            248..814
                     /locus_tag="KR440_003059"
     CDS             248..814
                     /locus_tag="KR440_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBH0700069.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRNRVNKND"
     gene            complement(1005..3041)
                     /locus_tag="KR440_003060"
     CDS             complement(1005..3041)
                     /locus_tag="KR440_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HBH0700070.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLKNVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGTFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYRLKDDKTSYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(3215..3892)
                     /locus_tag="KR440_003061"
     CDS             complement(3215..3892)
                     /locus_tag="KR440_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBH0700071.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNAVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSRLYFDLSTPGPNHNTKIDINPPS
                     FEKPLKK"
     gene            4587..4772
                     /locus_tag="KR440_003062"
     CDS             4587..4772
                     /locus_tag="KR440_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBH0700072.1"
                     /translation="MILLEQRKLRIGFNKSGKGSFTPRLILPMSWIKEMNISPDERDV
                     LVTFEDGKIIIEKTENE"
     gene            complement(4813..>6274)
                     /locus_tag="KR440_003063"
     CDS             complement(4813..>6274)
                     /locus_tag="KR440_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBH0700073.1"
                     /translation="NKELNSLECSGQYIEKSIIKNGENIVVDIGIENSKLKELYPQNY
                     NISTGFLTWENLSDFPNSISYSLEYEYGPVNDTETMDVNISIDKLVKCRIRYIDYDGS
                     IINEKEISDNSSINEIQINPQRENYEFSGWRNNKDEKIYTSEQVSKLVFDDSTDFIAT
                     YKRKSTGSSGSSGGTSSKEDFNIAPSKEKITGADRNETSVKISQKGWNKADNIVLIND
                     SSISDALSATPFAKSKDAPILLTKNNNLNKLTEKEINRLEAKNVYIVGGLKSVDEKVV
                     SDLKKKGLNVIRISGNDRYETSIKLAKELDKNSNLSKVVVVNGEKGLADAVSMGAISA
                     KEEMPILLTNQNDDMKDIKDLIANKNISKSYVIGGESLFNNKEVNNTLPSVTKIAGSD
                     RTETNSKVISHFYSKDTLNDLYVAKNGMNKQDDLVDALSVGVLAGKTESPVVLVGNGL
                     DDSQKELIKNKKFKNITQIGGNGNEKAFNEVENLVK"
BASE COUNT         2086 a          983 c          704 g         2501 t
ORIGIN      
        1 atttatatat tatattacaa gtattttcaa gtcaaaatct ttctaaaaaa tttaagaatt
       61 attaattcct catagaaatt atcattggta catagaagtt tttacataca attatacaat
      121 aatttaatta atagttacat aaaaaagtaa tactttaata tattttgaca ccatattgac
      181 attagaatta attcatgata ttctgttttc gacaaaacaa cttctaaaaa taaaggagat
      241 atatttcatg gattattcaa atgaactaaa agagctcttt ttaatgaatc aaacatatgc
      301 aactcttttt acccttacaa acaaaataca aatagaagga gataaatatt ttggtatact
      361 tacttcaaga cagtatatga ctattttatc aattcttcat ttaccagagg aagaaacaac
      421 attaaataat attgcaagaa aaatgggtac aagtaaacaa aatataaata gactagttgc
      481 taatcttgaa aaaaatggat atgttgatgt aattccaagt cctcatgata agagagcaat
      541 taatgttaaa gttacagact taggaaaaaa ggttatggta acatgtagta ggactggaat
      601 aaactttatg gcagatgtat ttcacgaatt taccaaagat gaattagaga ccttatggag
      661 tttactaaaa aaaatgtatc gttttaatgg tgaagaacaa gatggctttg aagaagatgc
      721 taattttatg gaatatgaag aaatagataa aattaaatct gaagcacttg aagaatttgc
      781 aaaaagaaga aatagggtaa ataaaaacga ttaatactat cgcatctata ataccacttt
      841 tttatagtac aatattttat ataactataa tgtcattttt attttttaac aaaacaatca
      901 ttacatagat taggtgttct taggaactct ttttctgttt tttgtacaaa aaaaggagat
      961 gattagactt ttattttaaa gcatctcctt tttcgctatt atgattattc tactcctaaa
     1021 atcatatctt ttaatttttg taaatcatta attggttgac tacaactatt actttgacac
     1081 acgtaataac tagtcttatc atccttcaat ctatagttat ttaaaaaacc tataatagtg
     1141 ttttcttcat tgtatttctt tgctaaaaaa gttgcatttg gtataaaatt ttcacttatc
     1201 aattctttaa atgcaattaa atcagaatct tccttaaaaa tacatataat ttccttagta
     1261 gaataaagtt caaacatcaa tgaaagcata taaaagctgt agccagttgg agaactcttt
     1321 acattatcca catacagttt taattgttta tatgacattt cttcaagtct attatctcca
     1381 gtaattttcg caagtcttat aagattgtat aattgaacag aatttcctga tggaatagct
     1441 ccatcataca aatcttttgg tcttgctatt aaattttcac tatctttacc atatatataa
     1501 aatccacttt tttcataatc ccaaaaaagg ttgatacagc tttcattcaa atttaaagcc
     1561 ttttctaaat atttcatatt aaatgttgac tcataaagct ctatatacgc ccagattaaa
     1621 aaagcataat catctaaata tgctaaatag tcggaacttc catctctata tctagctaat
     1681 aatctacctg actcatttac aagattatta tttataaagt taagacactt atttgaatac
     1741 tctaaatata tgtcattttt taaagtacta tatgcctttg ttaaagcaac tatcatcaat
     1801 gcattccatg atgtaagtat cttgtcatct ttatgcaaag atgttctttc ttttctatat
     1861 tcaaatacct ttttactaag gtcagctatc ttttcattat gtctttcata ttctttattt
     1921 tttataagat taggaatact tttaccttca aaatttccac tactagttat atcaaaataa
     1981 ttattaaaga aagttccatc ttcttcaccc aaaacttcaa ttatctcaag aggattaaat
     2041 gtataaaatt taccttcttc accttcacta tcagcatcct gtgctgaata aaacccacct
     2101 tctttatcct tcatctctct aactacataa tctatagttt ttatagcaat ttctttatat
     2161 aactctttct ttgttatttt atatgcatct aaaaaagcta tagtaagcat agcattatca
     2221 tatagcattt tttcaaaatg aggagccaac cactttttat cagtagagta tcttgaaaac
     2281 ccaaatccaa tatgatcaaa caagcctcct ctatacattc catctaaagt tttttccacc
     2341 atttttaaca catctttatc tttttctatg ctataatatt tcattaaaaa cataagattc
     2401 tgaggacttg gaaatttagg agcattacca aaaccaccat acttttcatc ataaatggcc
     2461 ttaaatactc taactgatga actcaacatc tcttttgata aatcaccctc agtatttttt
     2521 acaccaaaat catccttaag agcttctatt atttcatctc cagattttat taatatatcc
     2581 ctacttgtat tccatttttc agatacattc tttaataaat ctataactcc aggtctatta
     2641 taccttgaat attttgggaa atatgttccc gcaaaaaaag gttttttatc tggtgtcatt
     2701 attatggtca ttggccatcc accactacct gtcatagctt ggcaaactgt catatacacg
     2761 ctatctacat caggtctttc ctctttatct acttttattg ccacaaagtt tctattcatt
     2821 atttcagcaa cttcttcatc ttcaaaagat tctttttcca taacatgaca ccaatgacat
     2881 gttgaatatc ctacacttaa aaatattggt ttatcctctt cttttgcttt tttaaaagcc
     2941 tcatcattcc aactatacca gtttatagga ttataagcat gctgtaaaag atatggagat
     3001 ttttcattta ttagattatt aggttttcta ttactgtcca aaataccacc tcatttgtta
     3061 ttttattata tgttttgcaa taaacaaata gatatacact tataataagt gtatacccat
     3121 ttttaactat acatatttaa tttttcatat aattaattct tctagtaaat aatttttata
     3181 actactaaaa caaaacaaca taaaaaatat caaattattt ttttaaaggt ttttcaaaag
     3241 aaggaggatt aatatctatc ttagtattgt gatttggccc tggagtagac aaatcgaaat
     3301 ataatctaga atcttctgta actccaaaat ctctatttga acctgtatct ttaactttat
     3361 ttaaagcatc tctaaataga gcattatgtg cttcttctct gttaagtaag aaatctattg
     3421 tttcttttac atatttatca tctatttgtc tatacaagta ctcataaact accttagctc
     3481 tttgttcaga agctatattt gaaagtaagt cagctactaa atcccctgta actgtaacat
     3541 aattagcagt ccaaggagct cctgatgagt ttattaagac tggtgataga ccagttaata
     3601 catgtgtttc tatttcgcct gtattaactg cattataatc aacatcatgt ccatttaata
     3661 aatttatagt ttgtgctacc atttccatat ggctaagctc ttctgctgct atatcaagaa
     3721 ataaatcttt tatttgaggg tcttttattc taaaactttg ggacaagtat tgcatagctg
     3781 cttttaactc tccatttgca ccacccaact gttcttgcat taaaacagca tattgaggat
     3841 ttggtctttc tacttttact tcttttaata atgctttatc gtgtttaaac atatgaatac
     3901 ctccgtaaat tattattcaa aattattatt tacttaagtt gtaatattat tctaggttat
     3961 ttgtttattg ttgttatttt ttacaagtgt tggtatttca atgttttatt aaggtcagtt
     4021 ttgttgttta tgttttatta tgtttaattt aaatttattc tattatttat tttcggtggc
     4081 aaaatggtgg caagtttggt tttgtcacca tttattttta tttgtaaaaa atatagttta
     4141 tcaatataac tttattgaaa aatatgtaat atttattgac tttcacgtca cgtgatgata
     4201 taatataaat atagaaagga ggtaagaaag tggggagatt agaaagaaga aaaaataaaa
     4261 gagaaaacaa atttaataaa attaaaaatg ctttctcttt cacattagtt ttaattaatc
     4321 tagtactcgc aatactaaga ttgattaaag aattataagt ttcaatccta ggaggaaaat
     4381 accttccttc taggtagctt ctttctaata ttataacaca ttttctaatt taatatgaat
     4441 aataaatttt atagaatttg tatgattatt cttggtatca ctatagtttt aaatattatt
     4501 aatataataa taagttttac ttttaaagct cttattgctt tggtatttac tttattattg
     4561 ctggcattta ttaatagtag aaaggaatga tattattgga gcaaagaaaa cttagaattg
     4621 gatttaataa aagtggcaaa ggctctttta cacccagatt gatacttcca atgtcatgga
     4681 ttaaagagat gaatatctct cctgatgaaa gggatgttct tgttactttt gaggatggaa
     4741 aaataattat tgaaaaaact gaaaatgaat aaaaaaggag gtagtaacta tttccagtta
     4801 ctacctttca atctatttaa ctaagttttc tacttcatta aatgcttttt catttccatt
     4861 tccaccaatt tgagttatat ttttaaactt tttattcttg attaactctt tttggctatc
     4921 atctaatcca tttccaacta atactactgg agattcagtt ttacctgcaa gcactcctac
     4981 tgataaggca tctactaaat cgtcttgttt attcattcca ttcttagcta catataagtc
     5041 attaagtgta tctttgctat agaaatgact tattactttt gagttagttt ctgttctatc
     5101 agaacctgct attttagtta cagatggtaa tgtattattc acttctttat tattaaataa
     5161 agattctcca cctattacat atgattttga tatgttttta ttagctatta agtcttttat
     5221 atctttcata tcatcatttt gattagtaag aagaattggc atttcctctt ttgctgatat
     5281 agctcccata cttactgcat cagctagtcc cttttctcca tttaccacta ctacttttga
     5341 caggttagaa tttttgtcta attcttttgc aagttttatt gatgtttcat atctatcatt
     5401 tccagatatt ctaattacat ttaatccttt tttctttaaa tcagacacta ctttttcatc
     5461 aacagacttt aatccaccca ctatgtacac attttttgct tctaatctat ttatttcttt
     5521 ttctgttaat ttatttaaat tattattttt agtaagtaat attggtgcat ctttagactt
     5581 agcaaatggt gttgctgata aagcatccga tatgctagag tcatttatta acactatatt
     5641 atcagcttta ttccatcctt tttgacttat tttcactgat gtttcatttc tgtctgcacc
     5701 tgttattttt tccttagatg gtgctatatt aaaatcttct ttacttgaag ttccaccaga
     5761 agaaccactt gatcctgttg attttctttt ataagttgct ataaaatcag tactatcatc
     5821 aaaaactaat ttacttacct gctctgatgt atatattttt tcatctttat tgttcctcca
     5881 accactaaac tcatagtttt ctctttgagg attaatttga atttcattta tactagaatt
     5941 atctgatatt tctttttcat taattatact tccatcataa tctatatatc tgattctaca
     6001 tttcactaat ttatctattg atatatttac atccatagtc tctgtatcat ttactggacc
     6061 atattcatat tccaaactat aacttatgct atttggaaag tcagaaagat tttcccatgt
     6121 tagaaaacct gttgaaatat tataattttg tggatataat tcctttaatt tcgaattttc
     6181 aattcctata tctacaacta tattttcacc attttttata atgctttttt ctatatattg
     6241 accactacat tccagtgaat ttaattcttt attt
//
LOCUS       DAEPOZ010000210         6169 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.210, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000210 DAEPOZ010000000
VERSION     DAEPOZ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6169
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.210"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            182..1096
                     /locus_tag="KR440_003064"
     CDS             182..1096
                     /locus_tag="KR440_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700074.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGVKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            1120..1755
                     /locus_tag="KR440_003065"
     CDS             1120..1755
                     /locus_tag="KR440_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HBH0700075.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTAQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            2131..2847
                     /gene="pyrF"
                     /locus_tag="KR440_003066"
     CDS             2131..2847
                     /gene="pyrF"
                     /locus_tag="KR440_003066"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBH0700076.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVRAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGADFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            2873..3916
                     /gene="carA"
                     /locus_tag="KR440_003067"
     CDS             2873..3916
                     /gene="carA"
                     /locus_tag="KR440_003067"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBH0700077.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGEILTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIAPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            3969..>6169
                     /gene="carB"
                     /locus_tag="KR440_003068"
     CDS             3969..>6169
                     /gene="carB"
                     /locus_tag="KR440_003068"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HBH0700078.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYL"
BASE COUNT         2559 a          645 c         1224 g         1741 t
ORIGIN      
        1 gtatagacat tgataaggta gaatttaagc cttttgaatg ctatattaaa ctagagtaga
       61 tattcttatg aatttcaaaa tagggcagga ggaaaaagga aataaaacta taaaaaatat
      121 aaagtattta tataaattta tagagtttat acgtcagaag atttcagtta ggagggcata
      181 aatggagctt tatgaagcca ttttttatag aaaatctgta agaaattttt ccagtaagaa
      241 gataaaaaag ccattgatgg aagaaataaa aagaagttgt aaaaatttag aatacttaaa
      301 tgaagattta aatattaaag cacatgttgt tgatagaggt catataatac attttttgat
      361 gggtaaggaa tgtaaagtaa aggcacctca ttatatagta gttacatcag acaaaggtga
      421 ggactattta cagaatatag gatttgctac agaaggtgta gttttgcgcc ttacaacatt
      481 gggaatagct acttgctggt tggaaggaaa cttaaagaga gaagatatct tggaatttgt
      541 agatattggg aataagaatt ttgaagaaga tgaggaagat gatttagatt atctagctgg
      601 agtaaaaaca gaagaagatg atgaaaacaa aatagaaaat ccttatataa taatagcatt
      661 tggatatcca gcaaaaaatg aagagttgtt taggacgaga tatgctagac ctgatagaaa
      721 gccagtaaaa gatatgataa ataagataaa taggaataga aagtggaaag atatcttaga
      781 attgacaaga agagctccat ctgtaaaaaa ttctcaaccg tggtatttcc ataaggatga
      841 acgaggtctt catttatttg aaaagagacc taagaaacac tgtgaagata tgaataaggt
      901 tagcttaggt gtagccttaa gacattttga tatagcttgt ataaaaaata aaatagatgt
      961 ttcatatgaa aaacttccta taaggaataa aataggaaaa tcatatttta taactgtagt
     1021 cgagcatgtg aaaccagaag aagaaaccca agaagaaaat gtaactctag aaaaagaaga
     1081 atcacaagat gaataaaatt ttggaagggg atattggata tgtataagtg tggaaaatgt
     1141 aatcttagag gctgttctaa tggaaatatg gataaattaa tgaagatatg tccaactaga
     1201 gaaattgaac tacaacaaaa agcaaagcaa ctttatgaag aagaagagaa tagaaaaata
     1261 gcttataatg ctggattagt tgaagcagag gggtattgtc aatatactag actaaaagag
     1321 attatagagt ttgctaaaaa gtgtggttat aaaaagattg gcatagcatt ttgtattgga
     1381 ctaaaaagag aaatgcaaac agcacaaaaa atatttgaac atcatgggtt tgaagtattt
     1441 tctgttatat gtaaaaatgg ggcaatgcct aagagtacaa ttggactaga agcatctcaa
     1501 acaatatcaa agtgttcaga tgaggtaatg tgcaatccaa taggacaagc tttattttta
     1561 aatgaagcta aggttgacct taatgtaatt cttggattat gtgttggtca cgatacttta
     1621 gccatgaaat atatggaagc tcctataaca actttagttg tcaaagatag agtaactggg
     1681 cataatccag ttgctgcaat atataatgca gaatcatatt ttgagaaaaa attgtttgat
     1741 aaagatgaag aataattcaa agacttattg acataaaaag caattagtgg tttaattata
     1801 aaaaagataa aactttaaat tatcctatag atacataaag ttatataaat aaagaaaaat
     1861 attacagcta aattccttaa agtagtccag agagactaaa gggttataga taatgttagt
     1921 atataacctc tagtaaatct tctagaggtt tttgatgagt ataaaataaa aatttaaata
     1981 taataaagtc ttttaaacta gtccagagag actgggaaag atatctcata gctaatgaat
     2041 ggagttatgt atagataaaa tccttatgcc tcttttggtg tgaggatttt tttatgatta
     2101 tttaggcttt attaaaaagg agatgtaaaa atgataaatg gtaaagaaaa attgatagtt
     2161 gcaattgata ctgacgaatt tgataaagct aaagaactaa tagacagatt agaagatagt
     2221 gtagacatat ttaaggtagg tctagagcaa tacgttgcca caaaaggtaa aacaatagat
     2281 tatcttaaag aaaaagggaa aaaaatattt ttagacctta aattccatga tataccaaat
     2341 actatgaaaa gtgcagtgag agctgcagtg agagataatg tttggttaat gacaatacat
     2401 gtatcagata tggaaggaat gagacaatgt gcactaattg ctagggaaga agcagaaaga
     2461 ttaaatatag aaaagccatt agtagttgga gtaacagttt taacttcatt aagtaaccaa
     2521 gatttacagg acataggatg taatatgaca acagaagagt tagctataaa gagagctaaa
     2581 ctggcaaaag aatcaggcat agatggggtt gtttgttcag cgcaagaagt agataaaata
     2641 gtagaagcat gtggagctga ttttataaca gtttgcccag gaataagacc taaatcagca
     2701 gaggttggag accaaaaaag agttgtaact ccatcagatg caataaaaaa aggtgctcat
     2761 tatttagttg ttggaagacc tataacaaaa gcagaaaatc caagagaatc agctattaat
     2821 attgtaagag aaatagaaaa tgcttagata aaatatttgg gaggtagctt atatgaaggg
     2881 gaaattaatt ttagaagatg gaacagtatt tgaaggaaaa gcatttggat atctaaagga
     2941 agctattgga gaagttgtat ttaatacttc aatggctggg tatggagaaa tattgacaga
     3001 cgcaacttat tatggacaaa tagttactat gacttatcca cttataggaa actatggaat
     3061 aaatttagaa gatgttgaat caaataaggt gcaagtaaaa ggacttattg taagagaaaa
     3121 aagtgataac ccaaataatt ttagatgtga aatggatata gacacatatc taaagcaaaa
     3181 taaggtaata ggacttgaag gaatagatac aagagcatta actaaaatta ttagaaacaa
     3241 tggaactaag agaggaatta taactttaga aaatgtatca ttacaagatg ttcagtctaa
     3301 attagaagag cattgtaaca aagattcagt aaaaaatgtt acaagaaaag aaattgaaaa
     3361 tataaaaggt actggagcta aagttgtagt tatagacttt ggtgttaaaa atagtgtact
     3421 agagtctctt aaagcttgta actgtgatat aactatagca ccagcaatga ctacagtaga
     3481 agaaatttta aatataaatc ctgacttaat cttattatct aatggaccag gaaatccaga
     3541 ggatataaat gatatagttg aaaatataaa aaaattaata ggcaaaaagc cattgacagg
     3601 aataggttta ggacatcaaa ttttagcatt ggcattagga ggaaagactt caaagcttgt
     3661 atttggacat agaggaggta atcacccagt taaatgttta gaaactggaa aggtatttat
     3721 aacttctcaa aatcatggat ataatgtttc agaaatgcca gaaaatacag aggtaactca
     3781 cataaattta aatgacaata ctatagaagg aatgagacat aaggaattac caatatatag
     3841 tgttcagtac attccagaat acacaacaaa agaagattta gattgtatat ttaataaatt
     3901 cttagcatta atatagattt agtattattt acagatagta aaaaccattt agataaggag
     3961 aaaaatttat gcctaaatta gatagtataa agaaaacttt agtattaggg tcaggaccaa
     4021 taataatagg tcaagctgca gagtttgact attcaggaac acaagcttgc cagtctttga
     4081 aagaggaagg gattgaagtt gtattaataa atagtaatcc agccactata atgactgata
     4141 aagaagtggc agataaaata tatatagaac cattaacaat agaatttata gaaaaaataa
     4201 tagaaaaaga aagaccagac agtttacttg caggtatggg tggacaaaca gggctaaacc
     4261 tagcagttga actttatgaa aaaggcatac tagataaata tggagtaaag ataataggta
     4321 cttcagtaga atctatcaaa aaaggtgaag atagagatat atttagagaa gtaatgaaag
     4381 aaataaatca gcctgtaata gttagtgata tagttacaga tttacaagca ggtcttgatt
     4441 atgcacttac aattggttat ccagtagttg taagaccagc atacacacta ggtggaactg
     4501 gtggtggtat agctgataat gaggaagaat taagagaaat actttcacat ggtctacaat
     4561 taagtcctgt tggacaagta ttaattgaaa agagtataaa aggttggaaa gaaatagaat
     4621 atgaggtaat aagagattca aagggaaatt gtatagttgt atgtaacatg gaaaatatag
     4681 acccagtcgg agttcataca ggagacagta tagttgttgc tccaactcaa actttaagta
     4741 acaaagaatg tgcaatgctt aaaaaagcat ctttagatat attaaatgca gtagaagttc
     4801 aaggtggttg taacgttcaa tttgcactta atccacatag ttttgaatat gcagttatag
     4861 agataaatcc aagagttagt agatcatcag ctttagcatc aaaagctaca ggatatccaa
     4921 tagctaaagt ttcatcaaaa atagctttag gttatacttt agatgagata gaaaatgcag
     4981 taactaaaaa aacttatgct tgttttgaac caactataga ttatgtggtg gcaaaaatac
     5041 caaaatggcc ttttgataaa tttaaaaaag caaatagaaa attaggaact aagatgatgg
     5101 caactggtga aattatgagt ataggaagta actttgaggc tgctattctt aaagggataa
     5161 gatcacttga aacaggaaaa tattcattag ttcacacacc atctgaggag agaagcattg
     5221 aggaattaaa gaaaagagtt gtagtacctg atgatgagag actatttgac ttagctgaaa
     5281 tgataagaag aggctataaa gtagagatga tagaacaaat tacaggtata gataaatggt
     5341 ttataaataa atttaaatgg atagtagagc aagaagaaaa attaaaagtt atgaaaattg
     5401 aagatttaac taaagactat cttcttgaat taaagaaaaa gggattctct gataaaggta
     5461 tatctgactt aatgaagata agtccagaaa aattatatga attgagaagt ctatacaata
     5521 tcaaaccagc atataaaatg gttgatactt gtgcaggtga gatagatgct atatctcctt
     5581 actattattc aacttatgag caatatgatg aggtagttgt tagtgataag aaaaaggtga
     5641 tagtattagg ttctggtcca ataagaatag gtcaaggtat agaatttgac tattgttcag
     5701 ttcattgtgt aaaatcttta agaaaaatgg acatagaaac tataatcata aacaataacc
     5761 ctgaaactgt aagtacagac tttgatacat cagataagct gtattttgaa ccattaacag
     5821 aagaagaagt tttaagtata atagaaaaag aaaagccaga aggtgtaata ctacagtttg
     5881 gtgggcaaac agctataaaa ttggctaagt ttttacatga aaaaaatata cctatactgg
     5941 gaacagattt tgatgatata gatgcagcag aagatagaga aaaatttgat gatttactag
     6001 agagactaga tataaataga ccaaaaggaa agggtgtatg gtcacttaac gaaggtattg
     6061 aagttgctaa tgaattaggt tatccagtat tagttagacc atcttatgta cttggtggtc
     6121 aaggtatgga aataacttat aacgaagaaa aattatctca atatctaca
//
LOCUS       DAEPOZ010000211         6167 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.211, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000211 DAEPOZ010000000
VERSION     DAEPOZ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6167
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.211"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(608..1324)
                     /locus_tag="KR440_003069"
     CDS             complement(608..1324)
                     /locus_tag="KR440_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0700079.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSREIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
     gene            complement(1865..2665)
                     /locus_tag="KR440_003070"
     CDS             complement(1865..2665)
                     /locus_tag="KR440_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBH0700080.1"
                     /translation="MTFKNKKFTINKYEILSIILVLCLILTGFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNVGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            complement(2980..3711)
                     /locus_tag="KR440_003071"
     CDS             complement(2980..3711)
                     /locus_tag="KR440_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700081.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNPHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEVCAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            complement(3698..4393)
                     /locus_tag="KR440_003072"
     CDS             complement(3698..4393)
                     /locus_tag="KR440_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0700082.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            complement(4395..5186)
                     /locus_tag="KR440_003073"
     CDS             complement(4395..5186)
                     /locus_tag="KR440_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0700083.1"
                     /translation="MKNKFFTTLLTGFFAIVFFFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQVFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFISLTCLMLFEIFFNREVDKN"
     gene            complement(5196..6008)
                     /gene="modA"
                     /locus_tag="KR440_003074"
     CDS             complement(5196..6008)
                     /gene="modA"
                     /locus_tag="KR440_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH0700084.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
BASE COUNT         2155 a          856 c          747 g         2409 t
ORIGIN      
        1 aagatattgg ataatagctt gtacaactat agatattaaa aatactatag ctaatttttt
       61 atttatagtt tttttattat acatactaag taagtttgaa aagaatacat atgttataga
      121 catatctttt agattatagt tattatattt aatattttca tatattatat agactataga
      181 tataattata aaattgtaga ttattaaatt agtgttgtaa aacattttat tacttgtaaa
      241 tgcatgaaac cttaaggtta atagtatcaa agttaatata agtgtaagtt ctatttttac
      301 cttagaaaaa aggcttatga cattctctaa gaatttgact ttagtctgtt tatttttttt
      361 atacaatgta aaacatagta tagtcaataa aaaacttgat aatataatac ctatgcagac
      421 aaactctaat tctacttttg acaaatgtac aggagtaccg aaaaaataaa aataagggag
      481 agcaaactct ttataataac aattaatatt ataagaaaag ttattattca aatcataaac
      541 tgcttcaaat aatatagaaa ctaatagaaa cagtaataat aataaaataa attttgcttt
      601 agaatcatta ataattaagt ttttcaattt tgtaaccaat gccccacacc acctttaaat
      661 attttggttc ttttggatta atttcaattt tttctctaat tcttctaata tgaacagcaa
      721 tagttctctc acaactataa gcatcttcat tccagacatt ttcataaatt tcacgggaag
      781 agaaaatttt accttttcgt gacataagaa gtgtaagtat tttatattca agtggtgtca
      841 ttttaatttc ctcaccatct aaagaaacta tttttgtatc tgtatttagc tctaggccac
      901 cactttttag aatataatta gtatcatttg agttatttgt attactaaaa tcacctaaat
      961 ttatatacct tctaagttga gattttactc gagcagtaag ttctagtgga ttaaatggtt
     1021 tagttatata gtcatcagca cccatattaa gacctataat tttgtccata tcttctgatt
     1081 ttgcagataa gagaattatt ggtatattcc ttttttctct tattttaagt gtagttttaa
     1141 gcccatctaa attgggcatc attacatcca ttattattaa cttaatatca ttttcgatta
     1201 gtatgtccaa tgcttcaagt ccatcatatg cctttaaaac attaaagtta tcaggtctca
     1261 aatagaactc tatagcatca actatctctc tatcatcatc tacaaccaat atattttgat
     1321 tcaatttatt aacccccttg aaattttatt tttaaccaat ttttatttta taccaaaaag
     1381 aggtattaga aaacagtaag cccgttcatt atgaaataaa aactagtact tttaatatat
     1441 tgtcaatgca tatagtaaat ttaaaagtat agacgcccat attaatctga aggtaaaagt
     1501 ttatgaaaat atctagggta atctatcaca ctccttatgt tattttgggt ttattaattt
     1561 tttaggttta aacatatatt aacaaatata aataacaaat gagtttcata attattaaga
     1621 atttataaca aatatttatt ttaatgtagt ttaatataaa taggtagttt aatgtagata
     1681 tgtataaagt atttaataag gcttataggt tttaatgatg atatctttat atattttaag
     1741 agatgatgaa attagatatg ggtaatagtt gaatgagaaa aagtagtata gcacaaaaag
     1801 tgctatatca caaaatctca aataatttcc ttcttataac ttaaattaaa agaataaata
     1861 tttcttataa attaggtgta tgaagtatag caggaacatc tttattattt aataacttat
     1921 caaatgattt atggtcagac ttatagccaa taccttctct atattctaat tcatcaacaa
     1981 ttttgtattg ttgacaggca tttttaacct tttcatagtc ttcagtagaa tgagctttat
     2041 aaactatctt attattaaaa gttattttat cagcaaattt aggttttaca aaatatttag
     2101 catcttttat tttaccaaca ttatataaat tttggataac agaaccattt tttaaatgtt
     2161 gtaaatggtc atctaactta taagtatgtg ctatatgagt tacattttta aatttttctt
     2221 caaatgtaag agcaattttt tccatgtaat tgaagtcagt attaccattt gtatttgttt
     2281 ctggtaataa tattatattt tcccttatta caccaaggct attaagagaa gctaaaaatt
     2341 ctctatttct atattctgat ttttgtatat ttgttaggtt ttcatatttt ttatatttat
     2401 caaagacgga tataccacat ccagtagata ctaaaactac aaatacatga ttttttcctc
     2461 tttcttttat agcattaaca atagcactcc cagcccacaa cacttcatca tcttgatgct
     2521 gaacatagaa gacaacataa tctttaaatt ttttatgact ataattcaca tagctactaa
     2581 aaccggttaa aatcaagcat aaaactagaa taatagatag aatttcatat ttatttatag
     2641 taaatttttt attcttaaat gtcatttaat catctcctga aaagttgtct tagttatatt
     2701 atcaaacaaa gtactaaaaa ttatttctta aatgctgaaa aatatagagt agattaatgg
     2761 aataaaattt tttttattag tttaaaataa agaaaacatt tatgtataag actaatcaaa
     2821 atattaataa ttgataggaa ttgagattta aagggattgt acatttattc gggattcatt
     2881 ttaatagaat tttactttag tacttagaat aaaagtaata actttttaaa tttaaagagt
     2941 acacatatat atttgtgtac tccttgatga tatgattgct tatttactac aaaaacagag
     3001 tctttttaaa cctaaaatat ttgatgcacc agctatcgta gttccataat agtaaactgg
     3061 tttgttaagt gatagaaaat ttataattga gttattacat aatgtacttc ctgtgcaaat
     3121 cactaaatca gcccaagata ttacatcttc ataatctcgt ataccatctt caatcttaac
     3181 attatattga atagtatcca caaattcagg atttaaatct aatactcttg tttcgaaaaa
     3241 gtccttaata ttatcaatta tggctggttg atatcctata attgctactt ttggattacc
     3301 aaattccatt tttataaact cagggaattt ttttgcacat acctctggct cgttattttt
     3361 acaatgaatg gttctatccg tcttgcctaa gtatttcata actgcattta atgatgctat
     3421 aaaaagactt ctatcatgag gattgtcatg taaatctaat gataatatgt cttttaaaga
     3481 accttcaaat gtagatggag tagaagtaaa tgcttgtcct ttagctcctt taaagtcagc
     3541 ttcaagcatt atttctttac caactataat tgggaaatct ttacgcttag tatttccaat
     3601 tgcttctttt gaagatagag atttggacac aatacttatc atatccatat ttaaattata
     3661 tttatcgact tgtactaata gataatctct caaaatatca aatatattca tagaaaaaag
     3721 attctccttt attttttatt tcttcattta aatcaaaaat atgatttatt tttccattgt
     3781 tcattattcc tatcttatga gcaaaaaaca ttgcttcaga aatatcatga gtaacatgaa
     3841 tcactgttgg tttgtatttt atgtagacat ccttcaaaat tttgcttata tgtttttttg
     3901 taatataatc caatgaactc ataggttcat ctaaaagtaa tatatcaggt tcagctatta
     3961 aggcacgtgc aaaagcaact cgttgctttt cgcctccact taaattttta ggatacctat
     4021 tttttatatc ttctatctgc attaatttag acaagtggtt agccttaaga atctgttcat
     4081 ctataggtat ttttcttaat ttaagtggaa accttatatt atcttcaaca ttcatatttg
     4141 gaaataattc aaaattttga tatacaaatc ctatatttct attttctgga ggtaagtttg
     4201 atattaaatt ttctttgtat gatacatttc ctttagcaga tggatatctt cctgctaatg
     4261 tttctaggaa tagagtttta cctgacccac tagctcctaa taaaacaaag tattcatgat
     4321 tattaatatt taaactattt tcttgtaatt taaaattagg taaatcaaga gataaatttt
     4381 ttatttttaa catattaatt cttatcaacc tctctattaa aaaatatctc aaaaagcatc
     4441 aagcatgtaa gtgagataaa tatcaaaatt acagctattc cgattgcaat gtctatatca
     4501 ccagtagaca tatttaaata tatagatgta ggaataattt cagttttcat acgtgtagaa
     4561 cctgctatca tagctactgc accaaattca ccaagtgctc ttgaccaagt cattacagtt
     4621 gcagatatga ttccagtttt tagatttgga agaattataa atctaaatgc ttcccaacta
     4681 ttacatccaa gggtacgtgc aacaaatatc atcttttcat tactttcatt tagtgaagtg
     4741 tgaagtattt taatagtaag tggaagattt acaaatactt gtgctaaaac tataccttgc
     4801 tttgtaaata taaaatcaag cttaaaaatc ttataaatgg aatctccaat ccccattctt
     4861 ccaaacaaaa gtaaaagagc aattcctgat actaagtgtg gaagagacat agggagattt
     4921 attatctgta taatcagttt tttaaatggt aatttagtaa tatgtaaaaa atatgctact
     4981 ggaatagcta ataaaaggca tactatagta gatattaaag atgttttaat actaagtgtt
     5041 attgcatact gtacttccac ggattttaag catataggaa cataacttat ccctttaatg
     5101 attaacataa gaagtggtga tacaataaaa aaaaagacta tagcaaaaaa tccagtaagt
     5161 agtgtagtaa aaaatttatt tttcaagttt aatcactact taacaggttc aaaaccatat
     5221 tttttaaata tctgtttagc ttcatctgac tctatataat ttataaattt attagctaaa
     5281 tcttttttat cactatagtt tacgattcct gctgatatga gttggtctat atttttatct
     5341 tgggcaattt ctataacttc tacatcttca ttgccaaaga ccgaatcttt tgtggctatt
     5401 gtagcatcag ctttacttga tgttaactgt acaatcattt catttacagt tgcagctgta
     5461 gagacaacat tatcatttat agattttaaa ttattttttt caagtatttt ttgtgtagtc
     5521 ttacctatag cattagactc tttgtctccc aaaataagtt taactccatc tttatttaag
     5581 tcttccaatt tttgaatatt agctggattt cctttaggta caactattgc aggtgtatga
     5641 tgtgcaaccg ttttatgttc acctacaagt tctttttcaa aggcctttga ataaattggt
     5701 tcagaaccaa caataaatac gtctcctttg tgacttgtct caatttgagc tattagctgt
     5761 gcagaacctg catagctata ttcaactttg acattatttt ctttctcaaa tttttttgct
     5821 atttcatcca ttggtttctt taaacctgcc cctgaataga caaaaagagt ctctttttct
     5881 tttgaatcat cagctgttgt cactttagta ttagattcat cattagcttt gttttcttta
     5941 gaattacatg cagtaatact taatgaaata atcatagcta aagctagtac tgaaagtttt
     6001 ttaaacataa gatattcccc cttagtaaat tagtgtaaat tcttttttag tatatcatac
     6061 taagaaaacg atgtctaagt ttttgttatg ttttataatg ttttgttatg ttttattatg
     6121 tatagttaag aaaaaatcta tttataatgt catttccata ggtagtt
//
LOCUS       DAEPOZ010000212         6118 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.212, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000212 DAEPOZ010000000
VERSION     DAEPOZ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6118
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.212"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            90..1481
                     /locus_tag="KR440_003075"
     CDS             90..1481
                     /locus_tag="KR440_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0700085.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            1588..2628
                     /locus_tag="KR440_003076"
     CDS             1588..2628
                     /locus_tag="KR440_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBH0700086.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISIDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            3117..3770
                     /locus_tag="KR440_003077"
     CDS             3117..3770
                     /locus_tag="KR440_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBH0700087.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVSASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIHGIIDGMKF"
     gene            3846..4055
                     /locus_tag="KR440_003078"
     CDS             3846..4055
                     /locus_tag="KR440_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700088.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(4195..4761)
                     /locus_tag="KR440_003079"
     CDS             complement(4195..4761)
                     /locus_tag="KR440_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBH0700089.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSP
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            4922..5575
                     /locus_tag="KR440_003080"
     CDS             4922..5575
                     /locus_tag="KR440_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBH0700090.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(5733..5909)
                     /locus_tag="KR440_003081"
     CDS             complement(5733..5909)
                     /locus_tag="KR440_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700091.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
BASE COUNT         2372 a          642 c          956 g         2148 t
ORIGIN      
        1 atttaaaaag tagttaatat taggggaggg catttgcacc tcccttgtaa tatgtggaaa
       61 attgaatatt agaagaagga ggagacttga tggaaaatca acaattatta ggaacagaac
      121 gaattagtaa attactacta aaatattcta taccagctat aataggtatg ttggtaaata
      181 gtttatataa tgtagtagat agaattttta ttggaaatat accaggggtt ggaccacttg
      241 caataacagg tcttggtgta acaatgccaa taatgacaat aatacttgca tttgggatgt
      301 tgataggtat aggaactact actaccatat caataaaact tgggcaaggt aaagtagaag
      361 aggcaagaaa acttatagga aatgcaatga cgttatctgt aataacagga attatcataa
      421 tgatacttgg aatattattt gcaaataaaa tacttacatt gtttggagca agtgaaaata
      481 ctttaatata tgcaaaatca tatataaata ttatattatt aggtacagta gtaaatcttc
      541 tttccttttc tttaaaccac tcaataagag ctgatggtag tcctaaaatt tcagcaggaa
      601 taatgatagt aggatgttta actaatatag ttttagactg gatattaata tttggattca
      661 atttgggaat acaaggagct gcaattgcta cagtaacttc tcaggctctt acagctattt
      721 taactatagg gtattatata agtggaaaat caaacttacg attcagtaaa tctaatctaa
      781 aattagataa aaagctaata aaagcagtgt ttgcaatagg tatgtcgcca tttgcgatgc
      841 aacttgcagc aagtttagtt caagtaatat caaatattgc acttaaaact catggtggag
      901 atttagctat aggggctatg gctactattt cttccattgc aatggtattt ttgatgccta
      961 tctttggaat aaatcaagga gcgcaaccta ttataggttt caattatgga gctgaaaaat
     1021 atgatagggt taaaaaagca tatttaggtt cattagtagt agctactata attttatgta
     1081 tgggtatggt agtagttatg cttttcccag aagctattat aggtatattt aataaagacc
     1141 ctgaacttat gaatatatct gttaatggat taagaatata cttacttatg ttgccaattg
     1201 taggcttatc agttacagga acaaacttta tacaatctat aggaaaagca aaaatggcta
     1261 tgttattgag ccttttaaga caagtaattt tattgatacc agcagtatta attcttccta
     1321 cattcttagg tttacagggt gtatggacag ctcaaccagt gtctgatttt atagctacag
     1381 taattacagg tatagttgta tttagagaat taaaaagata tactcctaaa actgaaaaat
     1441 taaatgaaaa tgaaagacta aatgagataa ctactgagta aaaatataaa ttcagataat
     1501 tgagcaagaa attgcaaaaa atacgattat atgataaaat aaaaccgctg tcaaggataa
     1561 gagaaattct aattatggag tgattttatg gaatttacaa agaaaagcaa attaaaaaat
     1621 atgtatgtaa acatgaatgt agctagaatg ttgtctaaga taaatgagta taagggtagg
     1681 caactgcttt acaaaaaaca accaaaagaa attttagaaa acctagaaaa aaaatcttta
     1741 gtagattgtt ctgaatctac atacattgga aatcaaagag atagtagtaa ttttaatcta
     1801 gaaaagttga taagtaatga agttacacct agaagtagag aggaattgag tattgttgaa
     1861 tacagagatg tggtaaaaac tattaacagt gcttatgaga gtattcccat cagttcacaa
     1921 acgattcttg agcttcatgg gtacttatat aaattttcat ctactagagg aggaagttat
     1981 aagtcagata atgattttat tgaacacaat ttaaaaccta gcgaatttat aagtattgat
     2041 tcaagtgtca ataaagttga aaaagctgta gaagaaatat gtgaagctta taatacattg
     2101 atagaggaag atgaaattga tatattaata ttaatttctg catttgtgtt agatttcata
     2161 ttgatacatc cattcaaaga aggtaatata aaaatggcga gagtacttat cctattgtta
     2221 ctaaataaaa atgggtatga agttggaaga tatataagtt taggaaagat atttgatgat
     2281 agttcatatg aatattatag taatttaaat tatttgaaag cctcaatagg tagtgaaaaa
     2341 gcagatatga atgcatggat tgagtatttt ttagagacga tattaactgc atatgagaag
     2401 ctggatgata gtttaaatat atctgataag aaaagacaaa caaaaactag tagaatagaa
     2461 aaaataataa attctaccct tggatatttt acaaaagaag atataagaga tttatgtcct
     2521 gacattccag aacctactat aaatagagtt tttaataatc taagaaaaca agataagata
     2581 gaagttgttg caagaggaag aagtgcaaaa tggaagaaaa aatattaaaa aatgtttaaa
     2641 taactaaaat ggtatagtgt tttacactat accattttta atgtcattag catgagcaat
     2701 aatttaacaa cttaagcaat atcaatttta tttattgatt atttttgaac tgaaaaatgg
     2761 ggttatatgg tatactacaa taataattaa ttttggatga gaatatataa cgttacagaa
     2821 tatcttagga gtatatgtat aaaaaatata ataaaagtgt gaggtttagt aattaacaaa
     2881 ttatgtggct tcacactttt ttataaaata aaaatactta gcagaagaat attttagttg
     2941 atatgtttat aagatagtac ttgtttgggt aaaagtataa atagtaaaaa agtagaagta
     3001 atgtgtataa aaacataagt tacattctgt aataatagtt attttataat tcaacaaaag
     3061 aatagaatgg gtatatttgt gaaataataa tataaaataa tcttaggagt gatcttatga
     3121 agaaaaaatt attatatata aatgtaaatt caaaacctga agacttatct tcaagtaaaa
     3181 ctgtagcaag aaaatttata aataaattta tggaaaaaaa caaagatttt gaggttgaag
     3241 agatagacct ttataaagaa catataccga gacttgagta tcagtacttt gaaaaaagaa
     3301 atagcatagt tagtgaagaa aatgctaaaa atttagacga taaggataga aaagagctta
     3361 caaagattag aaatctttgt gaccaatttg tgagtgcaag tgtttatgta atagcagcac
     3421 ctatgtggag tttatctttt ccagcaccac ttaaagagta tatagattgt ataattcaag
     3481 atggaaaaac tatatctttt gaaggcaatg ataaaccaca aggaatttta aatgatattg
     3541 atagaagtat ggtatatata caatcttctg gtggacacat accatgggtc ttgaagccag
     3601 ttatgaataa agggttaaat tatgtggaaa gtattatgaa atttatagga attaaaaaat
     3661 ttgatgaatt gttagtagat ggaactggaa ctagtgaaga agaaagacaa gctgcaatag
     3721 aaaaagcatc tgaaaaaata catggtataa tagatggtat gaaattttaa ttataatatt
     3781 ttattaaaaa tataaaggaa aaacctcttt taatgtagaa acttatattg aaggaggcga
     3841 gaatcatgcg agaagaaaaa agtaatgaaa agtatgattg ttattggtgt aatcaagaaa
     3901 ataatttctg tgtagaaata aaagataata tagtaatgat agatgatggc actggtacgt
     3961 taaaacaagc cgttttcata gggtataaac aaatccaaat taatttaaat tgttcacatt
     4021 gtcaaaactt aaatagaata aaattaaatt tgtagaattt taaaatggaa attattgaag
     4081 gaattaaaaa tagaaagtgt taatatttag actgaaaatt atattttata caataaaagt
     4141 ttatttaaat aacattgtat tttaatattt attttcagtc ttactttatt aaaattattt
     4201 tttggttatg tctggaaaat atctgtctac taatttttct gaaatatttt ttaattggtc
     4261 agccattgaa gtcagactta ataaggctat caataaatca tctttatttt ctggatttat
     4321 actaatattt tcaaaattag actctataaa gtccggtata ttctttcttt gattttcttt
     4381 tattaacatg aatttttcgt aacatgaaac taaatctttt tcactgaaat tatccttttc
     4441 atacacacca tgtaagattc ttttaatttc ttgtattgta agtgtatttt tcatagaata
     4501 aataattatc atctgtaaaa tttgttcttt agtatacttt tttcccttaa ctggtttgat
     4561 taatccctct ttgctgtaat tatttatcat ggttttagta agaattttat cactttcaaa
     4621 tcttttatta ggagaaagtt tattgtcaat tagagaaata atttgatcca tatataagtc
     4681 aatagaggga atatcgtttg aagttatatc tgcattattt agtgtttcta gtattatagt
     4741 attaagttca ttattcatca tatgttacct ccataatata tataatacac taaattagaa
     4801 agtaaaacaa tattaagact tgtaaaaaaa acataaagtg gtataattaa atatataata
     4861 tagtaatgaa aactatattt atgaatctga tttttaaaat acataattgt aggaggacga
     4921 aatgtatcaa ttttttttaa agggtagaga cccaataagc agtttaactc attttatagg
     4981 tgcttgttta tctcttttag caactataat tttagttttt caatctgtta cattacagga
     5041 aacttctctc ttgatgatag tatctgtatc ggtatttgga ctttcactaa ttgctttata
     5101 tagtgcaagt tcgtattatc attttttaaa ggggacacca gaacaagaat tgttttttag
     5161 aaaagtagac catgcaatga tttatgtatt gattgctggc tcttatacac caatatgtct
     5221 gaattttatg gaaaaaaaag aaggcataat atttgttaca gcaatatgga tagttgcatt
     5281 tattggcata ataataaaaa tattttggat ggatgcacct agatggttat ctaccagtat
     5341 atatttactt atggggtggg caattgtttt tgatataaat gcattcaatt caattcctaa
     5401 ggattgcctt agactattaa ttatggaagg tgtatcttac tctataggtg caattattta
     5461 tataattaaa aagcctaata taagtcctga gtttggattt catgaaatat ttcatatttt
     5521 tattatgata ggttctttat ttcacttttt agcagtttta ttgtatgttt tatagtttta
     5581 aaagatttga ttaattgata ctaaaattct acaatgaata aaatggaact gccttatata
     5641 gattatatct tcgacaataa gacagttcct tattataaat attaaattct acttataata
     5701 aaattttgat aaaaatagtc cattcaaaat gcctattcca taattccttt aatgtacttt
     5761 tcatttacga catatagttt gaatttatgt actataatta tatcctctaa attctctgaa
     5821 acactaacaa ttctgacaca tttgtttcta atttctacag gtaaatctaa atcatttgtt
     5881 aaattcacat agtcatcata tttatacata aaatcatccc tttacattaa gatatactta
     5941 ttatactata ttttaataag tttttttaaa ttattacaat ttttcccgaa atttcatttt
     6001 ttatacataa aatttccaaa ttaggtaaag attaaatttt ttattgtata aacaaaattt
     6061 acatataaaa tattttattc aaatttttaa ataactattg ccaatttttg aaaaatat
//
LOCUS       DAEPOZ010000213         6116 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.213, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000213 DAEPOZ010000000
VERSION     DAEPOZ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6116
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.213"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..1686)
                     /locus_tag="KR440_003082"
     CDS             complement(<1..1686)
                     /locus_tag="KR440_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HBH0700092.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETHDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYL"
     gene            complement(1701..2909)
                     /locus_tag="KR440_003083"
     CDS             complement(1701..2909)
                     /locus_tag="KR440_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBH0700093.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            complement(2921..4597)
                     /locus_tag="KR440_003084"
     CDS             complement(2921..4597)
                     /locus_tag="KR440_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBH0700094.1"
                     /translation="MAKKVRIGDKLVEKGYITEDQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            complement(4748..5269)
                     /locus_tag="KR440_003085"
     CDS             complement(4748..5269)
                     /locus_tag="KR440_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBH0700095.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTPDGQTGLSVLETYMESLPDKADIGGKYKLIKVG
                     NKLVLQIGTNDEGVTLTEAQSAKLLSDIGENKIYTSVTADNLGNPLTSNTKVDNKVLY
                     IVLIDNTVMDSTK"
     regulatory      complement(5431..5516)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
BASE COUNT         1896 a         1137 c          624 g         2459 t
ORIGIN      
        1 caggtataat gtcatactaa atgtatagtt agaatcttct gcattacttt ttatgctatg
       61 taatttaaca tccttgaaat agtataatcc ctttaattta tataaaaatt gacctacata
      121 agaggaatct ttagcatttc cttctatatt tataacattt ggtatcttat catacaatgg
      181 agttttatca tctttatccg tgttattact attagtctca ctatttccag ttaatgcttc
      241 tgacacattt gtagtcttat cttcatttaa atcattatct gttttatctc cattactatc
      301 acttttcgta cttgattttg tttcattgtt acttgattta tctgttgtag tcttattact
      361 ttttgaatta tcagctttat tattagttcc aagattttga taagttgttg ataaacttgt
      421 tagttcaact ttacttggag tatttttatc tatatcatcc aataaatttt cccactttac
      481 ctttttccca attaacatat catatacctt tatttcttcg cttttactct gtatcatttt
      541 ttctatattt ccaatctgat aatatgcaga ttcaatattt cttatttcac cattaacatt
      601 ttcaagttcc ttatttgcta aattatttga aaaaattaaa cataaataag gtatagcaag
      661 aatcaaaata actattggag caattttcag tatgtttttc atttgtttag cttttatctg
      721 ttttctaagt atatccattg gtaaaaagtt tatattttca aaatctcttt tccctttagg
      781 ttctatcata cttcctaaaa gctctacaat tgaatttata tcatcttcat ctatatctcc
      841 agttatcata tcagatatat ccattatctc agtaaggcct tgagaaacat agcttgaaaa
      901 tgcagagcta aaagtttcat aaattaattt attggaatat ctttttccta atattaatat
      961 agtatccgta accttaccaa agtttcttga cgaatagtaa ttcataagac cttttgattc
     1021 ttctactaca tttaataatt gcatgtccat atctttagca gttatcttta taggtatatt
     1081 atcactaata aataaagtat ctttcttata tatatttata attgtactat tgtcacttat
     1141 attggttacc attatatcat tgtagtctaa atttttaagc attcttaagt aagctgttga
     1201 taatgtatct attctatcta atactaaatc aaattcatgc attatctcta caattgcatt
     1261 tacctcttcc ttttgtatag ctccaatgat aagatttaag cttgattcat cgccatctga
     1321 attatcatgt gtttcactag atacttcata cgaaaataca tattcatctg actccaaagc
     1381 catcatttcg tccatctcta gcttcataaa tccatctaaa tcctttttgt ccatattagg
     1441 aagtttttgg ggttttaaaa ttacatctct tgtatttaaa caaaaaacta catgtcttga
     1501 ttttaactta acattttcta atgcgtatct caatagagca tacttttcct tagaaaaatc
     1561 ttgtgcaata tcctctattg aaaacacatt aaaatttttt acataaaact tatccttatc
     1621 gtgtttatat ctgccctcaa ctatggaaat agaatcgcca taatatgata cataaacctt
     1681 attcatttgt cctttccctc ctacatggca tcatatatac taagcattgg cataaatatt
     1741 gatacaacca gtatcgcaac tataatacca actgcaatta ttaaaacagg ctctatcatt
     1801 gaggccatcc tatttaaata aatctcggat tcatactcat aaaattctgc catctgtcta
     1861 cttatattct ctaattcccc tgtctgctcc cctatagcta tcatctgtac caacacagag
     1921 tcaaaatacg tatatctctc taaattttct gctaagcctt ctcctcttgt tattccatct
     1981 ttagctgatg ctaaaatctt ctctgccatg gtgttattta cactatctat aatataatca
     2041 agcccttgaa ctaatgggta tcctgttgat acaaatagat acaatgctct tgaaaatcgt
     2101 gtagctacca tacaatttat tccttttcct aaaacaggta tcttgtttat aactctatct
     2161 ttatgagccc ttccaactga cgtttttaaa tattttttaa actgatagat aaataatata
     2221 ataactaaaa gtactattat ccaaaacttc ttcataaaca taccaaaccc cataacaatt
     2281 cttgttataa gtggaagagt tgctcctact gttgtaattg aattaatcat cttaggcacc
     2341 ataaatgttg taaatataaa aatcattata aatgaagaaa tcaaaactat taatggatat
     2401 gttgcagcat ttctaagctt ttgtttaacc ctgtgttcac ttgcataaaa tgaagccata
     2461 ctttttaata cttcatctaa tttacctgta gcttcacctg tagctaccat agcaccaagt
     2521 aactttggaa actttgattt tgatgaaagc attgaatctg caatagtaga acctactttt
     2581 atttctgcca ctatacgatt aatttcttct cttaatgtaa tgttagttga ccttctagct
     2641 aatgtttcta atcctgaaat actatttata ccagaagata ctataatagc aaactgtcta
     2701 caaaagtggc ttatctggtc agctcccaag tctttttttc tctttctact agataatctt
     2761 atatttttat ttttgctttc tttgatttca ataactctaa gatttctctt tctaagaaga
     2821 tttctagctt cattaaaatc gttggcttca atcacatcag tttgcttttt tccgtcctct
     2881 tttattactg tgtatttaaa acttttcata atatcatcct ttaatcagtc atcaaaatta
     2941 ttctaatcat ttcttctact gttgtctttc catttaaaac ctttttaact atatcctctt
     3001 ttaagctaac catgccttgt tttctagcca tctttaaaat ttctctttga tttgcagatg
     3061 aatctattaa ctcttttatt tctggagtaa tttcaagcat ttcaaatata cctagacgtc
     3121 ctttatatcc agtattatta cacctttcac aaccttttcc tctataaata gttactgatt
     3181 catttacatc aataccaaga gccttttttt ctccaatatc tgcttcatat gaaaccttac
     3241 aatgctcaca tatttttcgt gtaagtcttt gagatattac acctaaaaca gatgaagtta
     3301 ttaaaaaact ttccacatcc atatctaaaa gacgtgaaat tgctccaact gctgtatttg
     3361 catgtaatgt acttaaaact aaatgtcctg taagagaagc tctaattgct atttctgctg
     3421 tctcagtatc tcttatctct ccaaccatta ttatatctgg gtcttgacgc ataaaagctc
     3481 taagtccgct ggcgaaagta agccctgcct tttcatttat ttgagattga tttacaccag
     3541 gtaattcata ctctataggg tcttctattg ttaatagatt tctattttct gtatttagtt
     3601 gatttaacat tgaatacaat gtagtggttt tcccacttcc agtaggacca acaaccaata
     3661 taagaccata aggagtgttt atcaattcat catatatctt agagccatgt tcgtctattc
     3721 ctagtacttc tttactaacc ataaaattac tcttatcaag aaccctcata gctatctttt
     3781 catccctgtt agttggcatt gtagaaaccc taaaatctat gggtttatta tctactttta
     3841 aatagattct accatcctgt ggtattctct tttcagatat atttatatct gacattatct
     3901 tgatacggct gattactgcc ttatatggtg ctgagttcat cctcatatat tctcttaaca
     3961 ttccatctat cctaaaccta acacgcatgt aattttcact ttgttctata tgtatatcac
     4021 ttgcttccat tctaacagca ttttctagta tgttatttac taatcgtact attggagcag
     4081 catttacatc ttcttcaagt attactgttt ctcttactcc accagttcta tttttgttgt
     4141 actcagtcat tgccttattt atttctgaat gtgcataagc atgacctatt gctttgttta
     4201 tatcttctgt acttgatata aatatctcta cgtctttacc agacatacgc cttacatctt
     4261 gaactgcttc tctgtcttgt gggtctgaca tggctagaca tattttattt ccatcaattt
     4321 taaatggaaa tacagtatat cttttgcata tattttctgg aaccatcttt gtagctaaag
     4381 tgtctatttc tgtcccttct aaaaatatac tttctatatc caagagttct tttaatacac
     4441 ttattagtaa gttactatct ataagccctt cttgtactag aaattctcct agtctttttc
     4501 cactgttttt ttgttctgat agtgcccatt taagctggtc ttctgttata tatccttttt
     4561 cgaccagttt gtctcctatt ctaacttttt tagccacagg tttcactcct ttactatctc
     4621 aattatatca tcattcataa ttgaacatta aagaaaatag tataaaaaaa caggaagatt
     4681 ttcttcctgt cttttatatt atatttttca ttaaatttac aatattttta taaaaatata
     4741 gttttaacta ttttgttgag tccatcacag tattatctat aagtactata tatagaactt
     4801 tattatctac ttttgtatta cttgttaatg gatttcccaa attatctgct gtaacacttg
     4861 tatatatttt attttcacct atatcactca ataattttgc tgattgtgct tctgttaatg
     4921 taactccttc atcatttgta cctatctgta ataccaattt attaccaact ttaatcaatt
     4981 tatattttcc acctatatca gctttatcag gcagagactc catataagtc tctaaaacac
     5041 ttaaaccagt ttgaccatct ggtgtaactg gtatcttatt agtatctgaa taataagata
     5101 atgctgcact cttaactgaa ctataatcag actcaacact tgctacctta gccttgttta
     5161 tattactaaa taaagctgga actgccacta ctgctaatat acctataatt gcaattacta
     5221 ccaataattc cactaaagtg aaaccttttt tattcttttt taacttcatt tttattcccc
     5281 cttaaatttt ttaattaata cttacttata atatatctgg ttaaaaatct aagtaaaatt
     5341 atttaaacgt actaaccaaa tttatttcta acacataacc attttttaaa taaaaaaagc
     5401 tatgaaaatg atagtatcta taaataaata caatttcata gccggttgca ccactaactc
     5461 aatatgctct aaggtgccca cattacagaa cataaatcaa cgtttctatt ttattaaatt
     5521 caaaattata taactcataa tgtatataca tatccactta tattatataa tatctaccat
     5581 attttagcaa cactgtttat actatttata tataccaact ttttacatat tgaaacagaa
     5641 tttctagaaa aattatactc tacaaaaaaa agaaacatct ccctacaatt attatatatc
     5701 gtaaggagat atttataaat tatcatttag ttttatcatt gtcatttcca tcctttgcta
     5761 atatttattt gaaagctaaa agctattaca atatagtatt ttataaaatc atctattata
     5821 aacattctat ctattatagt tatgattcag ccacaatcat tatataaacg aaactcttca
     5881 tttacagcta tgcttattgt gcaaaattac tctttacagt aatcttatat aatccagaac
     5941 cctctcacaa acactaaaga tagaatagat actattttca attcttataa ataaactttc
     6001 aatagatttt tatattcact ttagattgtg actgaatcat ttaattatta ggggttcatc
     6061 ttctaacctt cccattggaa acacctcttt ctttatttat atttacataa tactac
//
LOCUS       DAEPOZ010000214         6087 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.214, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000214 DAEPOZ010000000
VERSION     DAEPOZ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6087
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.214"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            146..1369
                     /locus_tag="KR440_003086"
     CDS             146..1369
                     /locus_tag="KR440_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBH0700096.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTKESEPEKPIAVSVQKAVGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPNILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNANNKFKAGMSAQVNLSIEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            1373..4507
                     /locus_tag="KR440_003087"
     CDS             1373..4507
                     /locus_tag="KR440_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HBH0700097.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGEKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            4541..5839
                     /locus_tag="KR440_003088"
     CDS             4541..5839
                     /locus_tag="KR440_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HBH0700098.1"
                     /translation="MSKIVAIGCGVGIITANLIPVYADNNTSSINNVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVAPTKP
                     EQGNLTDDEYKVLMDKYEKDYKRYEKEVEAYNLERQTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGLITLT
                     DYKKQVVSNQDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
BASE COUNT         2404 a          799 c         1078 g         1806 t
ORIGIN      
        1 taataattat aaatttataa tcaaattaga gaattataaa ttgccaataa agggaagttt
       61 tttaattagt ctaaaactaa acttaaatta taagacactt taaattctac aacaagctag
      121 tgtgcaatat ataataggag gagatatgaa aagatattta atagttttct taatgtgtag
      181 ttcactgttt ttagttggat gtggaaaaac aaaagaatct gaacctgaga aaccaatagc
      241 agtttctgtt caaaaagcag taggtggaga aattgaaaac acaaattctt tctcaggaac
      301 tacaaaagtt aaagatgaaa cagctgttac tgcacagact gttggcacag ttcaagaagt
      361 gtatgtaaaa cttgggcaaa atgtaagaaa aggagatgaa ttattgagta taagcagtcc
      421 agagctagaa aatagtgtaa agcaaagtaa agcctcacta gatttagcaa aagcaagtta
      481 ttcaagtgcc actggtggtt cattagaggc acaagtaaat caggcaaaaa cagctcttga
      541 caatgcaaag attcaatatg aggagtctca aagaaattat gacaataata agattcttta
      601 tgaacaggaa gtcattagtt tagaccaatt taaaaaaata gagttttctt tagagcaaac
      661 aaaacaacag ttagattctg ctcaaagagc atacgataca gcaacttcaa aaagtatacc
      721 tcaagctaaa gcacttgcta aaaaacagct tgaccaagct caagtatcat ataatttagc
      781 aatgagtaat ttagacaaat taacattaac atctccagta gatggtacaa taacagctaa
      841 aaactttgat tcaaaggaaa tgataacaca atcacaacca gcttttatta tttctaatcc
      901 taatatacta gaagttgatt taaatgtagc agaatcagat ataggtaaat ttaagaaaga
      961 tggaaatgta gatgttataa ttgaagacca aaggatttta ggaaaaatag attatgtacc
     1021 ttcagtagtt gaccctcaga catctttata tcctgttaaa gtacttgtaa ataatgcaaa
     1081 caacaaattt aaggcaggta tgtctgcaca agtaaactta agtattgaaa aagaaaatgg
     1141 tgctgttaca gtgcctaaaa aagcaatatt tgaagaaaat ggtaaaaaat atgtatacat
     1201 agcaacaaaa gacaatatag caaagaaaca cttggttcaa actggaattg taacagagga
     1261 taaaatagaa attaaaagtg gtgtaagtga taaagataca gtgattattg gaggaataag
     1321 tcttatttct gacggaacta agatatttcc tgtagaaaag gagaagtaga ctatgaattt
     1381 aacacagact tccgttaaac gtcctctcac aatcataatg gtctttttag tattaatagt
     1441 ttttggtggt attggatata aaaaaatgtc aataaatcta atgcctgata tagaaatacc
     1501 agttgtaatg gtcatgacta cttggacagg tgctggtcct caagacgttg atgaacaagt
     1561 tagtcaaaaa gttgatgaaa gtttatctgc cgtttcaaat gtaaaatcta ctatatcaag
     1621 ttcacaagaa agtgtttcta tggtagttgc tcagtttgaa tttggaacta atttggatga
     1681 aataatgaat gatgttaggt ctaaagttga tgcattacag accagcctcc cagatgatgc
     1741 agcaaaacca acagttttaa agctggatat gaatgcacag gctataggtc aacttgtcat
     1801 atctggagga aatgaaaata gttcacaagc cttaagaaaa tacgctgaag atgtaattca
     1861 gcctaaaata gaatccatag atggtgttac ttcagcagac cttaaaggtg gagaaaaagc
     1921 acaggttaat gtaatagctg acccagctgt tctttcaaat tatggagtta gtttatccac
     1981 tataaaaggt gtcttatcct ctagtaataa aacattccct tatggttcaa ttactcaagg
     2041 tgaggataaa atagttttaa gagcaattga taaattagaa tccctagatg atataaaaca
     2101 gattcaaatt cctgtaaaag gaggaaatac agttagatta gataatcttt gtagtgtaga
     2161 ttatggatat gcagacaaag atagtattta tagatacaat ggaaaagaca gtttggtaat
     2221 agatatttct aaacaacaag atgccaacac tgtaaaagtt atgcagagtg catacaaata
     2281 cgtagatgaa ttgaataagg aaaatcctca atttaaaatt aaaattgcta atgatacaag
     2341 ttcatacata agtgaatcaa taaacagtgt aatgagcaac ttgcttataa gtgctgttat
     2401 cgcatttatt gtaatatttg ctttcctaaa aagtgtaaga gcttcactag ttgttgcagt
     2461 tgcaatacca atttctataa taggtgctat agcaattctc tactttactg gtgaaagttt
     2521 aaatcttatt actgcaagtt cattggtaat ttcagttggt atggttgttg ataatgctac
     2581 agttgtaatt gaaaatatat ttaaatatag gaaaagtggc agattgagtc ttgatgattg
     2641 tgctattgag ggaacaagaa ctgtaacaaa tgcaatactt gcatcaacat tgaccacgat
     2701 agcaatattc ttacctatca tgtttacaga aggtatagct aaaataactt ttggggcatt
     2761 aggtaaaaca ctaatagctt cactagcatt ctcctttata ggtgcaatta cactcgttcc
     2821 tagtgtattt gcaaaattaa gcagaggaaa aaatagtcaa aagatgcaag aaaaagatag
     2881 tcctatattt gataaggtaa gtgaagcata taaaaaatta ttaagtgtat gtttaagaca
     2941 tcaacgctta gttgtaatat caagtttact tatttttgta gcttccttat ttggaatggg
     3001 atttataggt atggacttta tgcctgcagc agacaagggt gaattatcta tttctataga
     3061 acttccaaaa ggtcttagcc tagaatccaa tgattactat gtatctatga ctgagaaaaa
     3121 agtctcagat attccagaaa ttaaaacttt aataacaact ttgacatcag attcaaataa
     3181 tagtaaagca agtatagcaa ttgaattaga atctcaaaaa aatagaaaaa gaactactga
     3241 tgaaatagaa aaagagataa ttgatagagt ggctactgta ccagattgta aaataaatgt
     3301 aagtcaaaac agtagtgtta tgggtggagc tggaggagca gacttcacat tagaaatgaa
     3361 aggtcctgat ttagatgtat taaaaatact gggaaaagaa gctgaaaaag aatttagctc
     3421 tataaaagga tttagaaatg ttgaaacttc tcttacagat acaacacaag aagctcaatt
     3481 tactatagat aaacataagg ctcaagaata tggtgtaaat actgctgaaa ttgcaggcat
     3541 gttacgtaca gcaataaatg gtgactccgt aaccactgct actattgatg attacaaagt
     3601 ggatgtaaac ctaaaattta aagcagatac tattgatagc ttagaagata taaagcaact
     3661 aaaagtaaac tctgcaactg gaaaagaagt tcctcttggt gctttttcag atataaaaat
     3721 ggcagatggt ctacaatcta tatctaggac tgatggagat ttttctgtaa atataactgc
     3781 tacacttgac aatttagatg ctggtactgc tacaaaacta gcactagaaa aaacgaataa
     3841 aatggtaatt cctaaagatt actctatagg tgttggtggt gaaacagaaa tgatgaatga
     3901 atctatggca agtcttggat tttcaatggt catagctata gcattagttt atatggtaat
     3961 ggtagctcag tttgaatcat ttagtaaacc atttataatc atgttctgtg ttccatttgc
     4021 attcgttgga gttgtgcttt cgcttctagt tagtggtcaa agtttaagta ctgtcggtat
     4081 gctaggtata atcttactta tcggtattgt tgttaataat ggtatagttc ttattgacta
     4141 catagagcaa cttagaaaaa caaatcgtaa caatgattta attggtttgg tttcaattgg
     4201 ttgtgcagca agattaagac cagtcttaat gactacagca acaacaattt taggtatgtt
     4261 gccaacagca ctatctcttg gtgaaggtgg agaaactatg caaccactag ccatagttat
     4321 aatgggtggt ctaactgtat ctacacttat aactttggtg cttataccta ctatatactt
     4381 aatatttgat aaatgggaaa ataagttttc agacaaaatg cctaaaatat ttggtaagat
     4441 aacagataaa ataggaagta taaaacgtaa aataataaag aaaccaaaat tgaaagctaa
     4501 agtttaaata gaatggaggg tgattgtatt gaataaaaaa atgagcaaaa tagttgcaat
     4561 tggctgtggt gttggtataa taactgccaa tttaatccca gtttacgcag ataataatac
     4621 ttcttctata aacaacgttg ataattctat tttaagtgac tcaaataaaa gaacatctga
     4681 aaaggaaatt ttgactgcta gagaagctgt tagtgctgca cttaaaaata gtgaaaaatt
     4741 aaaaatgaaa tcagaagaaa taaaaatgtt aaaagaaaag ttagaagtac aagatgagtt
     4801 tgatagtttt actggaagtg acaactcgtt tccatacgac caaattggac ttcttaaaaa
     4861 tcaaagtgaa caggctaaag gctttatgga ggaccaaata gcaaatgata ttactaataa
     4921 atttaacgat ttagtttcaa gagaaaatga gctagataag ataaaaaaca atcttgaaat
     4981 aaaaactaaa gaaattaaag atatgaaatt aaaaaaagat ttagggttag taacatcttt
     5041 agaaactgaa tcagctgaac ttgaacttca aacactacaa aatactcaaa aagctaaact
     5101 tcaagaacta aaaaacaatc aagactattt taagttattg actaatatag atttaaataa
     5161 ttatcaattg gataaagaat atagatttga atcttttaga gtaggtggct cagttgattc
     5221 atatatggaa ggtaaggtta atgaatattt aaaatacgac caattgattt tagaacgtac
     5281 agaagaaagc tttaatgata aagatgagaa caaagcagat ttaccagata gacctaatct
     5341 tacaagacca gttgctccta caaaaccaga gcaaggtaac ctaactgatg atgaatacaa
     5401 ggttcttatg gataagtatg aaaaagacta caagagatat gaaaaagaag ttgaagcata
     5461 caatcttgaa agacagactt atgctgctgg ccttactact tatgcaaatt atcttcaaca
     5521 aaaatttaat accgaaaatg gtctagtaac attagaagac agtaaaaaag ctcttaaaaa
     5581 agggttaatt gatagctatg ctcaattact tgctttagaa gatacaattc agattactaa
     5641 aaaacaactt gatttatcag aaaaacaatt aaaaaatact aaattaagat atgatttagg
     5701 attaattact ctaactgatt ataaaaaaca agtggtaagt aatcaagatg ctaaaaatag
     5761 ctatgacact cttattgtga attataactc actaaaaaat ggtatagaaa aaccttggat
     5821 acttaatact ggaaaataat tctggtaaat agaaagatta ctttaacata cgtttgatta
     5881 aaaaatagtt atgcacactc gtggctttag tcatggaaga ttcagaaggc aaataataga
     5941 aatagattag taaaaatata tttacttata aaactgtata cttttataga ggctatctca
     6001 aaattggata tgtccctgtc aagtagacag acttaaaaaa ggctatcttt atgcggaatg
     6061 agttcgatat tcaatcgaac tcattcc
//
LOCUS       DAEPOZ010000215         5987 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.215, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000215 DAEPOZ010000000
VERSION     DAEPOZ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5987
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.215"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..576
                     /locus_tag="KR440_003089"
     CDS             <1..576
                     /locus_tag="KR440_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBH0700099.1"
                     /translation="IIYSYKITSLYIFKNFKIGILLVFYFLFSQIIFGLIISDNQFDY
                     KNFLNYILLPIISLVYIYPFLCEEYGWRSFLQSILFDRFGKKIGIVMVGTCWSLWHLP
                     LQFTLYSPQTPIIGAIAHLIYGIGLSIFLGYVYMKTKNIWFCSIMHVLINSLGLIFND
                     SEIIINYHSIIQRLIFILLFYTPFLFTKEYK"
     gene            956..2482
                     /locus_tag="KR440_003090"
     CDS             956..2482
                     /locus_tag="KR440_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBH0700100.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKNIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NNGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            2510..3061
                     /locus_tag="KR440_003091"
     CDS             2510..3061
                     /locus_tag="KR440_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700101.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPYYSKKI"
     gene            3590..4633
                     /locus_tag="KR440_003092"
     CDS             3590..4633
                     /locus_tag="KR440_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700102.1"
                     /translation="MLKKWFGIVKKRQKSESVKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNEILTKVEVVNDDNEIEEKIIEEDWLISENTIKLDDKEAIINDKNIELCKKEVQ
                     VESEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLEYIKEIKIKRGKSIKAI
                     NLYTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKG
                     EWSYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYD
                     EYFKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTK
                     VGRNFIRYSLRSI"
     gene            4744..5886
                     /locus_tag="KR440_003093"
     CDS             4744..5886
                     /locus_tag="KR440_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBH0700103.1"
                     /translation="MSEEKNPKENTLDEPKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
BASE COUNT         2479 a          500 c         1019 g         1989 t
ORIGIN      
        1 attatttatt cttataaaat aacaagttta tatattttta aaaattttaa gataggtatt
       61 ttgctagttt tttacttttt attttctcaa attatttttg gattaataat aagtgataat
      121 caatttgatt ataagaattt cttgaattat attcttcttc ctataatatc attagtatat
      181 atttatcctt ttttatgtga agagtatggt tggagatctt ttttacaaag tattttattt
      241 gatagatttg gcaaaaaaat aggtattgta atggtaggca cttgttggag tttatggcac
      301 ttacctttac aatttacact atatagtcct caaactccaa taattggagc tatagcccat
      361 ttgatatatg gtattggact ttctatattt ttaggttatg tttatatgaa aactaagaac
      421 atatggtttt gctctataat gcatgttcta attaatagct taggattaat tttcaatgat
      481 tctgaaataa ttattaatta ccatagtatt atacaaagat tgatatttat actattattt
      541 tatactcctt ttctatttac aaaagaatat aagtaagaat tacaattaat ttatataaaa
      601 taaattttta agtaaatatt tttatagaga gattgttata ttacatatca gaaaaatatt
      661 aaacgaacta aatcaataaa aaagttggtg gctaacaatt tataattgtt agcacaatat
      721 aatcttatgt attgaaatct agaatatcct catttttact tagcataaat actattgatt
      781 aagaaaataa tatagaaatt ataaatatat ataagtttag tctggtgtat aaaacaagaa
      841 aactaagtga tttttcattt agctttttat taagcttatt ttttatacta tttataataa
      901 ataaactcca tgatgggaaa actattacta tttaatatgt aaaggagtat tttaaatgaa
      961 tgaatcatat tggtttttaa actcaagtcc aaaagaatat aacaaattag gtgaaaatat
     1021 aaaaacagat tgtcttatag taggtggtgg aataactgga ttgacaactg catatttatt
     1081 agcaaaagaa ggtaaaaaag tagtgcttgt tgaggctgat aaaataggat atggaacttc
     1141 tggaaggaat actggtaagg taacttgtca gcatgatata ttttactcta agatagagaa
     1201 aaaatatgga ttagataaag caaaatcata ttataatgca aataatgaag ctttaaattt
     1261 agttgaacaa ataatagaag aaaacaatat aaaatgtgat tttaaaaggg aaacttcttt
     1321 tgtatttaca gaaaaagagg atactataaa gaatattaaa gatgaatatc gtacatgtaa
     1381 aaaaatagga ataaattgtg agtatcatga aactatagaa aatattccat tagatataaa
     1441 gggggctatt agttttacta atcaaggtca atttaaccca aagaagtata ttgatggatt
     1501 ggcaaaagca gcagtaaatt tagggctaaa aatatatgaa aatacaccag tagtagatct
     1561 tgaaaaagga aaaatatgta gggttaaaac aagagaagat aatataatag aagctgagaa
     1621 tgttataatt tcatctcata gtccatggta tgatggattg aatttttatt ttgcaaaaga
     1681 atatgcagag cgtgcttatt taatggttgc agttttggaa aataaattag cagatggtat
     1741 gtttataagt atagatgacc catcaataac ttttagacaa tataataatg gaagtgaaaa
     1801 tttactgatt tttggaggag gagaccataa agttggccag ggaggaactg agaaagaaat
     1861 atttgatgat ttagagcatt atggtaaaga agtctttaag gtaaaagatt ttaaggggaa
     1921 atggtctgct caagataata tgagttttga caatgttcct tatattggtt atatcaacaa
     1981 aagagaagac aatatttatg tagctacagg attctctaaa tggggaatta ctaatggaac
     2041 tgctgctgga ataataataa aagatttgat aataaataat aattctgatt ataaagatac
     2101 ttttaatcca tccagattag gtagctactt ttcaaaagat tttataaagg aaaatgcaaa
     2161 cgtagcaata aattatgtaa gtggaaaatt aaaaataggt agtggagata tgcctaaaaa
     2221 taatggagaa ggaaagattg ttaatattga tggaaaaaga tatggtgtat ataaggatga
     2281 taatggagat ttttatattg tagacacgac atgtactcat ttaggatgtg aacttaattt
     2341 taacagtgaa gaaaaaacat gggactgtcc atgtcatggt tctagatttg actataaggg
     2401 aaatatactt gaaggaccag ctcttaagcc attaaaactt tatggacatg gtgataatga
     2461 tgttaatcct aagttattat aaaattattt ttaggaaggt aaagtgtata tgaataaact
     2521 tttaaagcca ataactatac tattgtatat agtttatatt ttatctgccg tattttttgt
     2581 agcaaacaaa gaatatggaa gtctaggtct agtagcaatt agtttagttg gtactttcat
     2641 tttgagtttt ataaataagc aaactgaaag attattgagt aaagaattat atataatgct
     2701 tgctttattt ataatgtttt catcattgtt aggtacttgt tttaattttt atgatataaa
     2761 ttattatgat gattttttgc atttatggtc aggtataata tcttgtattg tggctttttc
     2821 tataattaaa tacttttata cacaaagaga gataagtaaa atgagtaaag tgtttttagt
     2881 tatattttta tttatgttta gtatgggagt agcgagttta tgggaaatag gtgaattttt
     2941 aatggatact tttgttggga ctactacaca ggccgggggg ttagaagata ctatgattga
     3001 tatggtagat gctttaatag gcacaattat aactattcct tattatagta aaaaaatcta
     3061 atatatagtt ttaaattttt tatattaatt ataatggatt taattaatca tgttgagttt
     3121 caaggtctat atgattaatt aaatccattt ttgttattta taattattat tgaatgtatt
     3181 tttacatata agatataagg tgatagatag aaattgttgg attatgtaaa taattaatat
     3241 ataataccat tatgggcaat aaatacaaat caaatgataa tatagtctat ttatgtaaat
     3301 gttatattgt atcctaagta taatagtaag ttttttgata gtggtgtaga aagtaaaaat
     3361 ctactagcct aaattgatgt atggattagt ttaggaacag taattgcata tcttaagttt
     3421 gatgtcatgt tgggctacta gaaataggtc atctttttca cttgaatatc ttttctttaa
     3481 acatgaggag tatcatatca aaaacatatg aaaattaagt gatttataag aacagtaata
     3541 ttttatatga tagtatagaa tagtaataaa gtattaggag gaacattgag tgttaaaaaa
     3601 gtggtttggt attgtgaaaa aaagacaaaa aagtgagtca gtaaaagaag aaggtgaagt
     3661 aatattaaaa aacgaaaaaa tattatctga agaaaagttg atagatgaag aaggagttgt
     3721 agttaatatt gataatgaaa tattaactaa agtagaagta gtaaatgatg ataatgaaat
     3781 agaagaaaaa ataatagaag aagattggtt aattagtgaa aataccataa aattagatga
     3841 taaagaagca attattaatg ataaaaacat agaattatgt aaaaaagaag ttcaagttga
     3901 aagtgaaaaa ataggtttaa ataagtttga aggacttgac caaaatgaaa attataatct
     3961 agaaaaaaat gttattgaag aaaaaaaagt aagtgaatgt ttgacagaag aagatttaga
     4021 gtacataaaa gaaattaaaa taaaaagagg aaaaagtata aaagctataa acttgtatac
     4081 taaagaagag tgggtttttg acactcatat acagtgtagt aaaaaactca aagttccatt
     4141 aggatacata agagaaaatt taaaatatgg atatatggat tactttggag atgcaataaa
     4201 ttatttaagt gaagtattaa atatagatga atactgcaaa ggtgaatgga gctatctcga
     4261 taatagcaaa tctccatctg agatatttaa tattctaaac aataaaatat ttagtataag
     4321 gctttctaat gaaaaaagaa atgaaatttt gacaaatgat aaaattgaag cattaaaaat
     4381 gaattataga tttgaatgta ttgatgaaga atatgatgaa tattttaaaa aatataagtc
     4441 tataatcaaa aggggtggaa agaaaaaagt tgaattagta aataaaaaag gtgacatttt
     4501 agaaatattt aagtctttag aagaatgtgc catttatttg caaaaggaaa agaatgaagt
     4561 tatacaaatg ttaaaatatg gagatacaaa agtaggaaga aactttataa ggtatagttt
     4621 gagaagtatt taatttcatt ttaaattcat aagagtttca tttctgattc acacatttaa
     4681 tattatttta atgtaaaata ataaaggtga agtattgcac aattaaaatt aaggagggta
     4741 ttaatgtctg aagaaaaaaa tccaaaagaa aatacattag atgaacctaa aaattcagat
     4801 aatgtaaatt ctaaaaaaga tgatgtgtca gatgaaaaaa atactgataa caaagttaaa
     4861 aaaagggaaa taaagctaaa caacaaaaca cttaaagcta ttgtagcaat tgttgcagta
     4921 ggagctatat gtttaggtgg aggctatgtt tttggtaaag aagctggaag aaaattgcct
     4981 gctacttcaa aacactataa tagcagtgag gtagtagcta ctattgggga taaaaaaata
     5041 acaggtgaaa tgttaagaaa gagaatggag ccattatttt atttaaatgc aaaaactact
     5101 atgactgatg acgaaatacg ttcatatgaa tcaaatatga ttgaaactat gattacatct
     5161 gatatattat cctctgaagc agcaaaagag aaaataagta taactaatga ccaactagat
     5221 gctcagtata ataatttaat gagtagtata gaatcagcaa tgggaatgac taaagaagag
     5281 tttttaaaac agtttgattt gactgaaaaa tatataaaag atgatatgaa aaaagagcta
     5341 gtagcatcaa aatatttaaa tgaaaaatca aaagttactg ataaagaagc agagaactat
     5401 tacaataaaa acaagagcaa ttatttacaa gttagagctt cacatatttt aataaaaact
     5461 gttgatgata aaggtaagca agttagtagt agcaaaaaag ctgaacttaa gaaagaagct
     5521 gaagaaatac ttaaaaaagc ccaagctggg gaagattttg ctactctagc taaaaaatat
     5581 tctgaagata gtagtgcaga atcaggtgga gatttaggtt tctttggtaa aggtcagatg
     5641 gtggaatctt ttgaaaaagc agcctttgca ttgaagaagg gagaggtatc taacaaactt
     5701 gtagaaagtg attatggata tcatataatt aagaaaactg atgaaaaata tcaaccattt
     5761 gaagaggtaa aatcagattt agtaagcagt ttaacatcag aaaaacaaaa cctacttata
     5821 caaaatttaa aagaaaaata taatgttaaa attacagata aatatcaaat gaaagctaaa
     5881 gaatagataa agtcaattta ttaaaaaaaa gaaaaattaa ctgtatggat aaaagctatt
     5941 tttactctca atatagagag tctaaagtag cttttattat tttgtaa
//
LOCUS       DAEPOZ010000216         5983 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.216, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000216 DAEPOZ010000000
VERSION     DAEPOZ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5983
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.216"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(25..2301)
                     /gene="slpA"
                     /locus_tag="KR440_003094"
     CDS             complement(25..2301)
                     /gene="slpA"
                     /locus_tag="KR440_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HBH0700104.1"
                     /translation="MNKKNIAIAMSGLTVLASAAPVFAAEDMSKVETGDQGYTVVQSK
                     YKKAVEQLQKGLLDGSITEIKIFFEGTLASTIKVGAELSAEDASKLLFTQVDNKLDNL
                     GDGDYVDFLISSPAEGDKVTTSKLVALKNLTGGTSAIKVATSSIIGEVENAGTPGAKN
                     TAPSSAAVMSMSDVFDTAFTDSTETAVKLTIKDAMKTKKFGLVDGTTYSTGLQFADGK
                     TEKIVKLGDSDTINLAKELIITPASANDQAATIEFAKPTTQSGSPVITKLRILNAKEE
                     TIDIDASSSKTAQDLAKKYVFNKTDLNTLYRVLNGDEADTNRLVEEVSGKYQVVLYPE
                     GKRVTTKSAAKASIADENSPVKLTLKSDKKKDLKDYVDDLRTYNNGYSNAIEVAGEDR
                     IETAIALSQKYYNSDDENAIFRDSVDNVVLVGGNAIVDGLVASPLASEKKAPLLLTSK
                     DKLDSSVKAEIKRVMNIKSTTGINTSKKVYLAGGVNSISKEVENELKDMGLKVTRLAG
                     DDRYETSLKIADEVGLDNDKAFVVGGTGLADAMSIAPVASQLRNANGKMDLADGDATP
                     IVVVDGKAKTINDDVKDFLDDSQVDIIGGENSVSKDVENAIDDATGKSPDRYSGDDRQ
                     ATNAKVIKESSYYQDNLNNDKKVVNFFVAKDGSTKEDQLVDALAAAPVAANFGVTLNS
                     DGKPVDKDGKVLTGSDNDKNKLVSPAPIVLATDSLSSDQSVSISKVLDKDNGENLVQV
                     GKGIATSVINKLKDLLSM"
     gene            complement(2527..4116)
                     /locus_tag="KR440_003095"
     CDS             complement(2527..4116)
                     /locus_tag="KR440_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp12"
                     /protein_id="HBH0700105.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETAAK
                     IAQKQTYENVVLVNTDNTLADGLSASGLAGTVKAPILLSQRNSIPSDTEKMLKDVKKV
                     YIIGTEDSIGKSVENELKQKGIEVKRIGGNDRIETSYLIAKEIASIKPIDKVFITNGY
                     TGEADAMSASSVASRDGSPIILTNGKNVPFEKKEGVQCYALGSEEIISNDLVKKTNAV
                     RLAGEDRFETNKKVIKHFYSSAKEFYLSKGYQLVDAVAGSSIAKNAPIVLVDGNSDKS
                     VLRSADKITALGGIDEKTLEQCLSASSLDASAPTITVGNLNIYQGDKFDISKLNIVAK
                     DSNGNDLTPELIGNIDTDKVGKYKVTIKATDIGGKTTSISVEVNVLEYKTNDMNSSEF
                     KRMVSSEMYSLVNSYRKEKGKEPLQVSKNLEGLSNSWSKYMADKGQFSHVINGKKSVE
                     VFTGYGLRSEENIAFVPLVTKSTYTTKDAREVANVIFTVWKKSDKYNENMLNSDFVYT
                     GFGLYILPNGEVYATQEFLNK"
     gene            complement(4335..5936)
                     /locus_tag="KR440_003096"
     CDS             complement(4335..5936)
                     /locus_tag="KR440_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HBH0700106.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADRQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEINEKVIQQCINASKSNGQPPTITVGSTEIYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSYDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLVVSSRLEGMANAWSKYMMDKKVFAHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKNVQTTQDAKNLAKAIFEVWKKSPEYNANMLSDEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
BASE COUNT         1716 a         1167 c          653 g         2447 t
ORIGIN      
        1 cctttttata ttattatatt tatattacat acttaataaa tcttttaatt tatttataac
       61 tgaagtagct atacctttac caacttgaac taagttttct ccattatctt tatcaagaac
      121 tttacttata gatacacttt gatctgaaga taaagaatca gtagctaata ctataggtgc
      181 tggagatact aatttatttt tatcattatc agaaccagtt aatactttac catctttatc
      241 tactggctta ccatcagaat taagagttac accaaagttt gctgcaactg gagctgctgc
      301 taaagcatca actaattgat cttctttagt agaaccatct ttagctacaa agaaattaac
      361 tactttttta tcattattta agttatcttg ataataagaa gattctttta taacttttgc
      421 attagttgct tgtctatcat ctccactata tctatctgga gatttacctg tagcatcatc
      481 tattgcattt tcaacatctt tagatacact gttttctcca cctattatat caacttgtga
      541 atcatctaag aaatctttta catcatcatt tatagtttta gcttttccat ctacaactac
      601 tattggtgta gcatcaccat cagctaaatc cattttacca ttagcatttc ttaattgaga
      661 tgcaactgga gctatactca tggcatctgc taatcctgtt cctccaacta caaatgcttt
      721 atcattatca agacctactt catcagctat ttttagagaa gtttcatatc tatcatctcc
      781 tgctaatctt gtaactttaa gtcccatatc ttttaattca ttttctactt ctttagatat
      841 agaattaact ccaccagcta aataaacttt ctttgaagta tttatacctg ttgtactctt
      901 tatattcata actctcttta tttcagcttt tacgcttgaa tctaatttat cttttgaagt
      961 taataataaa ggagctttct tttcagaagc taaaggagaa gctacaagtc catcaactat
     1021 tgcatttcct ccaaccaata ctacattatc aactgaatct ctaaatatag cattttcatc
     1081 atcagagtta taatattttt gacttaatgc tattgcagtt tctattctat cttctcctgc
     1141 tacttctata gcatttgaat atccattatt atatgttctt aaatcatcca cataatcttt
     1201 taagtctttc ttcttatctg acttaagagt taatttaact ggtgaatttt catcagcaat
     1261 tgaagccttt gcagcactct tagttgtaac tctttttcct tctggataaa gaaccacttg
     1321 atattttcca ctaacttctt ctactaatct attagtatct gcttcatccc catttaatac
     1381 tctgtaaaga gtatttaaat ctgttttatt aaatacatat tttttagcta aatcttgtgc
     1441 tgttttacta gaactagcat caatatctat tgtctcttct tttgcattca atattctaag
     1501 tttagttatt actgggcttc cagattgtgt tgttggttta gcaaactcaa tagtcgcagc
     1561 ttgatcattt gcacttgcag gtgttattat taattctttg gctaaattta tagtatcact
     1621 atctcctaat ttaacaattt tttctgtttt tccatctgca aattgaagac ctgttgaata
     1681 agtagttcca tcaactaaac caaacttttt agttttcata gcatctttta tagtaagttt
     1741 cacagcagtt tcagttgaat ctgtaaaagc tgtatcaaat acatctgaca tagacataac
     1801 tgcagcacta cttggagctg tattttttgc tcccggagta ccagcatttt cgacttcacc
     1861 aataatacta cttgtagcta cttttattgc actagttcca cctgttaaat tttttaatgc
     1921 aacaagttta cttgtagtta ctttatctcc ctctgctgga gagcttatta agaaatctac
     1981 ataatcccca tcacctaaat tgtctaattt attatctact tgtgtaaaca ataatttact
     2041 tgcatcttct gcactaagct cagctcctac ttttatagta gatgctaaag ttccctcaaa
     2101 gaaaatttta atctctgtta tacttccatc taataaccct ttttgtaatt gttcaactgc
     2161 tttcttatac ttgctctgta ctactgtata tccttgatca ccagtctcaa ctttcgacat
     2221 atcttctgct gcaaacacag gtgctgcaga agctaatact gttaatcctg acatagctat
     2281 tgctatattc ttcttattca tttcttaaat tcctcccaac attatattaa aattacatct
     2341 cttcattatg tacatagaat aatcatccat atattcctaa tatttctcac atctctataa
     2401 taatccattt ttattctttt tgcaagtcat ttttttgaaa ttattatttt tcccaagcat
     2461 atgagttttt atacccttaa actccaatat ttaataactt aaaaaatttg attttactta
     2521 tttaaattac ttatttaaaa attcttgtgt agcatacact tctccattag gcaatatata
     2581 taatccaaac ccagtatata caaaatcact atttaacata ttttcattat atttatctga
     2641 ctttttccaa actgtaaata ttacatttgc tacttctcta gcatcctttg ttgtataagt
     2701 actttttgta acaagtggta caaaagctat attttcttca cttctcaagc catatccagt
     2761 aaatacttca acagatttct taccatttat tacatgtgaa aactgacctt tatctgccat
     2821 gtacttagac caggaattag aaagtccttc taagttttta gaaacttgta atggctcttt
     2881 acctttttct tttctatatg aatttaccaa actatacatt tcactagaaa ccattctttt
     2941 aaactcacta ctattcatat cattagtctt atattcaagt acatttactt ctacacttat
     3001 tgatgtagtc tttccaccaa tatcagtagc ttttatagta actttgtatt tacccacctt
     3061 atcagtatca atatttccta taagctctgg tgtcaaatcg ttaccattgc tatcctttgc
     3121 tacgatattc agtttactta tatcaaactt atcaccttga taaatattta aatttccaac
     3181 agttattgtt ggtgcacttg catccagtga tgatgcactt aaacattgtt ctagtgtctt
     3241 ttcatctatt cctcctagtg ctgtaatctt atcagcactt ctaagtacag acttatcact
     3301 atttccgtca actaatacaa taggtgcatt ttttgctata gaagaaccag caactgcatc
     3361 tactaattga taacctttag aaagataaaa ttcttttgcc gaactataaa aatgttttat
     3421 aactttttta ttagtctcaa atctatcttc tccagctaat ctaactgcat tagttttttt
     3481 aactaaatca ttacttatta tctcctctga accaagagca taacattgca caccttcttt
     3541 tttctcaaat ggaacatttt taccattagt taaaattata ggtgatccat ctcttgatgc
     3601 tactgatgac gcactcatag catctgcttc acctgtataa ccattagtta taaaaacctt
     3661 atctataggt tttattgaag ctatttcttt tgctataaga taacttgttt caattctatc
     3721 attaccacct attcttttaa cttctattcc tttttgcttt aattcatttt caactgattt
     3781 tcctattgaa tcttcagttc ctattatata aaccttcttt acatctttta acattttttc
     3841 tgtgtctgat ggtatactgt ttctttgtga taataatata ggtgctttaa ctgttcctgc
     3901 taatccactt gcacttaatc catcagctag tgtattatct gtatttacta atactacatt
     3961 ttcatatgtt tgtttctgtg caattttggc agctgtctca tatctatctt tcccttgtat
     4021 cttttctatg ctcgaaagtg cattgatatt tggtgctcct gccattatta atgatattgt
     4081 caatcctatt gataatactc tcttatttac cttcatcgaa ctcctcctac taattttttt
     4141 atataattca gctaatcata aattacatcc tcagtagtga ttccctcaaa atctataaac
     4201 tactaaaaca aaaaacacct aaaataccat agcctgtaaa gcaataacaa aacagattac
     4261 aaaaagtact ttagatgtga ttttcatttt ataatatcag taaattaaca aaaaaacaag
     4321 atttatggaa ttttttacaa actaccttca ttgccattta aaaactcctg agtagcatgc
     4381 acttgaccat cagataatat ataaagccca aatccagtac tatagaactc atcactaagc
     4441 atgtttgcat tgtactctgg agacttcttc cacacttcaa atatagcctt tgccaagttt
     4501 tttgcatctt gtgtcgtttg aacgttctta gaatctatat aaatatatgc tatattttcc
     4561 tcacttctca ttccaaactc agaaaatact tgtggagcat tttttccatc tatataatgt
     4621 gcaaatactt ttttatccat catatactta gaccatgcat ttgccatacc ttctagccta
     4681 ctagatacta ccaaaggctc tttgcctttt tcttttctat aggaattgat taagttatac
     4741 atttcagtag ataccatttt tttaaattct gggctgttgt aatcatagga tttatcatcc
     4801 aacactttta tttctactct ttttcctgta ctttttcccc attcgtctgt agcttttaat
     4861 gtcaatatgt atgtacctac tctatttgta tctataaacc catcaacttc tattggtaat
     4921 accttcccag tgttatcttt agccactatg tttaacttgc cagtgtcaaa cttttcaccc
     4981 ttatatatct ctgtacttcc aactgtaatt gttggtggtt gtccatttga ctttgaagca
     5041 tttatacact gttgtattac cttctcattt atttcaccta cagaagttat attcttagct
     5101 ccttctaata cagttttatc gcttccatca tttactagta ccattggaga gtttttagta
     5161 agtggtgcag ctgctattgc atctactaat tggtatccat ctgatacata gaatccatct
     5221 gcactattaa aaaagtagtc tattacattt ttgttagttt caaatcggtc agttccttct
     5281 attcttactg agtttgtatt tttaactaga gggttactca ttatctcctc tgaacctata
     5341 caatatgatt gaatattttt tacatcaaaa ggcacactct ttccatctgt aagtatgatt
     5401 ggagctccat ctctagtagc tactgatgat acactcattg cgtctgcttc tccactatat
     5461 gcgttagtta atagtacttt atccactttt tttatagttg ctatttcttt agctattaaa
     5521 tagcttgttt ttagtctatc ttctccacca attcttttga cttctatatt tttagaatct
     5581 agttcttttt ctactgattt acttatagta tcttctgtac ctattatata tgcttttttg
     5641 atatttttta gacatctatt tgtgtctgct ggtatcttat tttgttgtgt aaatagtatt
     5701 ggagcttttg tagctcccga taatccactt gcacttaatc catctgcaag tgatttttct
     5761 gtatttacta aaattactgt ttcataagtc tgtctatcag ctatctttgc tgctgtttca
     5821 tatctatctt taccgtatat tttttctact actgatgatg catttacact tttgaagttt
     5881 actaagatta atgatactgc tagacctaat gataatattt ttttgttcat tttcatatcc
     5941 ccctgatgat ttttatattt tatatttata ttgtgattat ttt
//
LOCUS       DAEPOZ010000217         5948 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.217, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000217 DAEPOZ010000000
VERSION     DAEPOZ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5948
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.217"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            213..3056
                     /gene="mutS"
                     /locus_tag="KR440_003097"
     CDS             213..3056
                     /gene="mutS"
                     /locus_tag="KR440_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBH0700107.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIEKLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALMKQNDDKEKINQEDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            3067..5034
                     /gene="mutL"
                     /locus_tag="KR440_003098"
     CDS             3067..5034
                     /gene="mutL"
                     /locus_tag="KR440_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBH0700108.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKDKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYSVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNVDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            5072..>5948
                     /gene="miaA"
                     /locus_tag="KR440_003099"
     CDS             5072..>5948
                     /gene="miaA"
                     /locus_tag="KR440_003099"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBH0700109.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDIDAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINEKGKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMIKNG
                     LVEEVKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTARWFDLDQY"
BASE COUNT         2584 a          609 c          984 g         1771 t
ORIGIN      
        1 accttgaaaa tatcattaaa cttaaaaaag aaataatatc tttattgtaa aaatatatat
       61 atttttattt aataagcatt aaacttagca atccaaaatg tgagtataca ttttggattg
      121 tttttagtta tatgtcaaaa ttttatatat tgtgttaata ataatcatct actatgatat
      181 aatatttaat tagaaaagaa ggaggtttga taatgcaaat agatatgaat aaattaactc
      241 ctatgatgaa acagtatctt gaagtaaaaa atagatataa agactgtata ctgtttttta
      301 gacttggaga tttttatgaa atgttttttg aagatgcatt agtagcttca aaagctcttg
      361 aaatagcatt gacaggaaaa gcttgtggtt tagaagaacg tgctcctatg tgtggtgttc
      421 ctttccactc agccaattca tatatatcaa aattagttga aaatggatat aaagtagcaa
      481 taggtgaaca aatggaagac ccatctacag caaaaggaat tgttagaaga gaggttataa
      541 gagtcatcac tcctggtaca gttttagatg gaaatttact tgaaaataaa aagaataatt
      601 atttattatc tttgtataag gatggtacta atataggtct aacttatgtt gatataagta
      661 caggagaaac taatgcaaca tgtttaaatg aagataaggt aattgaagaa attgcaaaaa
      721 tccatccaac tgaaataatt ataaatgatt tagactttat agaaaagctt agagatatag
      781 ctactgtaag taatatatac ataaatgaaa gttttagtga taactatttg gatataaata
      841 tattaaaaga atattttcca gatgtttatc tacaaaaact aaagtttgat gataaaggtc
      901 taataaagtc aagcttatca attcttctga attatatata caatactcaa aaacaaataa
      961 catctaacat taataatata aatatataca attcatcaga atatatggtt ttagatatgt
     1021 ttactagaac taatttagaa cttactcaaa ctataagagg gaataagaaa aaaggttcat
     1081 tattacatgt acttgataaa acttctacag caatgggagg tagactttta agaaaatatg
     1141 tagaagaacc tttgataaat aaatctaaaa ttgaaaatag attagatgta atagaagaaa
     1201 taaaagatga ctttatatta agagaagacc taaatgatat acttaaaaat atatacgata
     1261 tagaaagaat atgtggaaag atagcatttg agagagtaac accaaaagag ttaattcact
     1321 taaaaaattc gatagaaaag ttacctaatt taaaagatac tataaattta tcaaatgcaa
     1381 aaatcctaaa agagtatgtt tcagaaatgg ataaattaga tgatatatac aatttaatag
     1441 atgaagctat tctagaagaa cctacgataa ccataaaaga tggaaacata attaaatcag
     1501 attttagtga tgaacttaag gaactaagag agatttctaa gaatggagcc tttcttgtaa
     1561 aggaaataga aaacagggaa agagaaaaaa caggagttaa atcattaaaa ataggattta
     1621 ataaagtttt tggatattat atagaaataa caaaagccaa ctttaaacag gcaaagctag
     1681 atgagacata tataagaaag caaacactta gtaatgcaga aagatatata actccagaat
     1741 taaaggaaat agaagaaaag atactacatg cagaggaaaa gattaaatct ttagaatacg
     1801 agatatttgt agagataaga gatacaatat ataaaaacat agatagaata caaaaagtgg
     1861 ctaagactat agctaatata gatgtttttg tgtcccttgc aacagttgca catataaata
     1921 actatgtaaa accagcaata aatgaaaata ataaacttga tataagaaat ggtagacatc
     1981 ctgttgtcga aaatatagta ggagaggaga atttcgttcc aaatgatact tatttaaatc
     2041 gtggagaaaa cattattaac ataataactg gtcctaatat gtcaggtaaa tcaacatata
     2101 tgagacaaac tgctataata gctcttatgg cacatatagg ctcatttgta ccagcagagt
     2161 ctgctgatat tccaatatta gatagaatat ttacaagagt tggagctagt gacgatttat
     2221 ctcaaggtca atctactttt atggtagaga tgaatgaagt atcccttata ttaaaaaatg
     2281 caacagagag aagtttagtt atattagatg aaataggtcg tggaactagt acctatgatg
     2341 ggataagttt agcatggtca atagtggaat atattcaaaa aaatattaga tgtaagacat
     2401 tatttgcaac acattatcat gaacttactg atttagaaga agagtttaaa gaggttaaaa
     2461 attattcaat agcagtcaaa gaagatgggg aaggaataat atttttaaga aaaataattc
     2521 ctcagggagc agacaagagt tatggaatat atgttgctaa attagctaaa ttaccagacg
     2581 aagtcataga aagagcaaaa tatatactta aagacttgga aaaaaatcat gtttacaaca
     2641 gtgttgcaat aaatggagat aaagaaaaca ataatataat taatagtaat ttggacgaag
     2701 aattagttaa ggtaaatcaa agtaatttta agaataaata tgaatctcta aaaatagaac
     2761 atgaacttat agtaaaagac tacaaacata taaaaaagga ttatgataaa ttaaattcta
     2821 aatttaaagc attaaatgat gaagttgctt taatgaagca aaatgatgat aaagagaaaa
     2881 ttaatcaaga agattctgta aaagaagtag ccttaactca aatctctttt gatagtgtaa
     2941 atagagatat actatcagaa gaaattttaa atctggatat tttaaatatg actccattag
     3001 atgctataaa ctctctttat aatcttcaaa gaaaagcaaa agaaataaaa tcttagaggt
     3061 gtaaaaatga aaaatataat taacatccta gatgatttga ctataaataa gatagcagca
     3121 ggtgaagttg tagaaagacc ttcttctgta gtaaaagaac ttattgaaaa ctccattgat
     3181 gcaggtgcaa ataaaatttc catagatata attgatggag gaaaaagtct aattaagatt
     3241 acagataatg gaataggaat accttcaagt gaagttgaaa aatcattttt gagacatgct
     3301 actagtaaaa ttaaaaaaat tgatgatttg tatgatttat actctcttgg ttttagagga
     3361 gaagcattgg catctatttc agctgtatca aaattagaaa tgacgactaa aactaaagat
     3421 gaaataatag gaactaaaat atatgttgaa ggcggaaaaa taatatcaaa agaaccaata
     3481 ggttctacta atggaactac catcattata aaagatattt tctttaatac accagcaaga
     3541 cagaaatttt taaaatctac tcatgcagaa actataaaca taagtgattt gataaataaa
     3601 cttgcaatag gtaatccaaa cattcaattt aaatatacaa ataacaataa acagatgtta
     3661 aatactccag gggatggaaa acttgtaaac actattagaa gtatatatgg aaaagaaata
     3721 acggaaaata taatagatgt agagtttaag tgcaatcact ttaaaatgaa tggatatata
     3781 ggtaataaca atatataccg ttcaaataaa aacttacaac atatctatat aaataaaaga
     3841 tttgtaaaaa gtaaaataat catagatgca ataacagaaa gttataagtc tataattcca
     3901 ataggtaaac atgcagtgtg cttcttaaat atagaagtag acccatcatg tatagatgta
     3961 aatatacacc ccaataaact tgaaattaaa tttgaaaaag aacaagaagt gtatatagaa
     4021 cttagagatt ttttaaaggt taaattaata cactcaaatc ttattggaaa atatgctact
     4081 tatagtgata aaaaaacaca acctagaatc gctattaaca gtagagaaaa aagtacagat
     4141 tataaattaa gaaataatga tttattagaa tctactccta aaaatagtaa tattactaaa
     4201 gataaggatg aagtcattga agttgtgact ttatcaagtg aaaaaccaat aaatgaattt
     4261 caatcagtaa gtgaagtcct aaatgcaagt gtagaagatg atgtaaaaaa cattaattat
     4321 ctaagtgaag atagtgcaaa tgataatata caagaagagt ttcaagttga tggaattaaa
     4381 aatgaaggaa attattactt aggagatagt attaaggaca gtgaagaaga atacttgtgt
     4441 agttcaaaac gcaagttttc cttatatgga tattctgtaa ttggagttgt atttaatact
     4501 tatataattt tgagcaaaga cgattctatg tatttgttgg accaacatgc tgcacatgaa
     4561 agaatactat atgaaagata tatggagaag ttctatagac aagatatcaa tatgcaaata
     4621 cttttagacc cagtggttat tgaagtatca aatgtagata tgcttcaaat tgaaaataac
     4681 ttggagttat ttatgaaatt tggttttgaa ctagaaattt ttggcaataa tcatataatg
     4741 gtaagatgtg ttcctactat atttggagta cctgaaactg agaaatttat acttcaaata
     4801 attgataata tagaagaaat tacaagtaat tatgatttaa aaggagagag atttgcatct
     4861 atggcatgta ggtctgctat aaaagcaaat gataaaatat acgatattga aataaaaagt
     4921 ttattggaac agctagaaaa gtgtgagaat ccatttacat gccctcatgg aagacctata
     4981 atggtcgaaa tatctaagac agaaatagaa aaaatgttta aaagaattat gtaaaatatt
     5041 tggatattta aaatatatgg aaaggaatgt tatgaaaaaa ataccactta taatacttac
     5101 aggtccaact gctgttggaa aaacagattt gtcaataaaa ttagcaaaag atatagatgc
     5161 agagataatt tcagctgatt ctatgcaaat atatgaatat atggatattg gaagtgcaaa
     5221 agttacagag gaagaaatgc aaggagtgaa gcattacctt atagatgaag ttaagcctga
     5281 ctatccattt tcagtttcag agtttcaaca aagagcaaaa aaatatatac atgaaataaa
     5341 tgaaaaggga aaatgtgttt tagtaactgg aggcacagga ctttatttaa actctttaat
     5401 atataatatg gattttgcac aatctgatgc caataatgaa ttaagagaag aacttcaaaa
     5461 acagcttgct gaaaagggaa tagattacat gcataataag ttaaaagaac ttgatgaaga
     5521 atctgctaat agaatccata aaaacaatac taaaagggtc ataagagctc ttgaagtttg
     5581 tctaagtggt aagaaaatga atgatttttc aagtgattta aaatttaatg aagaatatca
     5641 accaataata atagttctaa atagagatag agaacatttg tatcaaagaa taaatatgag
     5701 agttgatatt atgattaaaa atggattggt tgaagaggta aaaaaacttt taagtatggg
     5761 atttaaaaaa gatatgatat caatgcaagg tataggttat aaagagatat tgaagtatct
     5821 ggatggagaa tatacatacg aagaagctat tgaaataatt aaaagagatt ctagaaggta
     5881 tgcaaaacgt cagataacat ggtttaaaag atataaaaca gcaagatggt ttgatttaga
     5941 ccaatatg
//
LOCUS       DAEPOZ010000218         5948 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.218, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000218 DAEPOZ010000000
VERSION     DAEPOZ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5948
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.218"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(129..677)
                     /locus_tag="KR440_003100"
     CDS             complement(129..677)
                     /locus_tag="KR440_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700110.1"
                     /translation="MIFLNENVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTIEILRKVLFERSEERAIAMRERALENGDELIADNIISTTDVPFLGWVPEFKE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
     gene            complement(785..1450)
                     /locus_tag="KR440_003101"
     CDS             complement(785..1450)
                     /locus_tag="KR440_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0700111.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDVYKTTLIHMETPKYINLEDFC
                     NNK"
     gene            1846..2751
                     /gene="garR"
                     /locus_tag="KR440_003102"
     CDS             1846..2751
                     /gene="garR"
                     /locus_tag="KR440_003102"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBH0700112.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTILPNGTIVQDILFGMDGLAKTLKEGSIVVDMSSVTP
                     TESILCANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDAKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHNN"
     gene            2887..4269
                     /locus_tag="KR440_003103"
     CDS             2887..4269
                     /locus_tag="KR440_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBH0700113.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAVLIGGWIYGKRFEKYPAYAIDENDREKN
                     LEKGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKRYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            4340..5776
                     /locus_tag="KR440_003104"
     CDS             4340..5776
                     /locus_tag="KR440_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBH0700114.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNELLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPVETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTHGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNSGKEFMFRSAAAI
                     TKVLGGVSDKEMLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISSGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
BASE COUNT         2184 a          695 c         1042 g         2027 t
ORIGIN      
        1 tatgataatt catatagatt aaaagtaaat tatctttagg ttaaacaaaa gtaaaataaa
       61 agactagtta taatatttat atgaaaaagt acaaaatata gtatatgaat atagaaaatt
      121 taaattattt atttacagtt tttatttcca taagtatatt taccttcaga taccttgtca
      181 tcatggaaat atattctttc acaactttta tctccccata gaatgttttt tgttatttta
      241 tgggacgtat ctcctgtaaa tacttcagct acagtagtat ctgtaacatc acagtaaaga
      301 gaagcgaatt ttttaaacca agggttttcc tcaaatctta aaagccagga agctccataa
      361 gggcaatgta actctttaaa ttcaggaacc catcctagaa atggaacatc agttgtgcta
      421 ataatattgt ctgcaattaa ttcatctcca ttttcaagag ctctttctct catagcaata
      481 gctctttctt cagacctttc aaataaaact tttcttagta tttcgatagt tttatcttca
      541 ccaaactctt caaataacac tttggaaaaa taaaaatata agtcaccaaa atgtttgcat
      601 gccatacgaa cttcttcaat agcataatct ttgtcaatag tttcattagt tacaacattt
      661 tcgttcaaaa aaatcactcc ttagaatagt attagttaca atataattat agaatatttt
      721 ttataaatta ggaaattaat ttgaggtaga tatattttta ataaatgaaa gtataatact
      781 aaaatcactt attgttacaa aaatcttcta aattaatgta tttaggagtt tccatatgaa
      841 tgagagtagt tttgtaaaca tcttcaatac atccatttgc aatattatta aaaatatctg
      901 tgtgttcttt taatgttgtt tcaagccttc cttcatgtga taatgaatcg agagctaatt
      961 tttttattct gtacatatag ttgttaaaca ttttgtcaaa ttcagtattt tgagcatatg
     1021 aaactatagt tgtgtgaaat aatgtatcgt attctgcaaa tgagtttaaa tttttatcac
     1081 ctagtaaaat atctttttgt ttatctaaaa gatgttttaa tttacttata gtttctactg
     1141 ccctagcttc tttgtattga cttgtaatta aaaaagtaca aaacccttct attgcagagc
     1201 gtatttggaa tatctctaca atttcatttg ctgtaatttg atggattcta aatcctttgc
     1261 ttgggattat atctataaat ccatcttgac taagtctttg aagagcatct cttataggtg
     1321 ttctagagat acctatttca ctagcaagtt ttgtttcaga atatatttca ttttctatca
     1381 tttctccaga tagtatcaat tcttttaagt agtcatatgc ttgttcttgt aatcttttaa
     1441 atgtagccat acttaataat acccccctaa acaatataaa aatacaaaaa cttatataat
     1501 tttaagcata caacatagtg aaaataaggt caatcttttg caatttaaat tgcagataag
     1561 attaaaaaat ataaaaaatt attgaagtgt gaagtatgat taacattaaa ggcagattaa
     1621 ttaaaactaa ttgaaattag taaaaataca aaactgtaaa agtagcatat tatatttaaa
     1681 aacaatagta tgtattaaaa cttatatagg atgactaaat ataataaatg actttaaaaa
     1741 ttactattta ttgttaaaat aaatactttt atattttata ttgaccggta tactggtatg
     1801 tagtatactc taaaacataa aaaacgtttt caaaaatgtg aagatatgga gggttataaa
     1861 atgaaattgg gatttattgg attaggaata atgggaaagc caatggcaaa aaatttatta
     1921 aaagatggat gtaatctttt ggtgtatgac attaataaat ctgctgtgga tgaacttata
     1981 tcatgtggag caaaatacgc aagtgtttta gaaatgggtc aagaatgtga tattgtattt
     2041 acaatccttc caaatggaac tattgttcag gatatattat ttgggatgga tggattggca
     2101 aaaactttaa aagaaggaag tatagtagtc gatatgagtt cagttactcc tacagaatca
     2161 atattgtgtg caaataaatt aaaagatatg ggcttagaat ttatagactc accagtaagt
     2221 ggaggggagc caaaagcgat tgatggtaca cttgctttta tggctggtgg taaggaagaa
     2281 atttataaga aagtagagcc attttttaat ataatgggaa gctcatctat tttaattgga
     2341 gataatggta gtggaagtgt tacaaaactt acaaaccaag taatagttaa tttgacgata
     2401 gcagcagttt ctgaggcatt tgtacttgca gcaaaagcag gtgcagaccc agaaaaagta
     2461 tataaggcaa taagaggagg cctagctggt agtacaatat tggatgcgaa gataccaatg
     2521 attatgaatc gtgattttaa accaggtgga aaaatatcta ttaatttgaa agatattaag
     2581 aatgtaatgc aaacagcaca caatttagat gtaccgctac ctatgacaag tcaattatta
     2641 gaaattatgc agacattaaa agtacatgga catttggaag atgaccatag tggaatagca
     2701 cagtattttg aaaagttggc aggtgttgaa ataaaaaaac acaataatta atatttaaat
     2761 atatcaatat aattaaatac atattattag gattagatta tataaataca gcgaggttaa
     2821 gttatcatag taaaagaaaa ataaaattga taaataataa agtagaatga taaaggggta
     2881 aagattatga ttacaggata tggacttttg atagcatttg ttatatctat aggaatttta
     2941 cttgtatcga ttattaaatt taaagtaaat ccgtttttgg cattactgat aacatctata
     3001 attacaggct ttatggttag aatgcctata aatgaaatat caaccacaat atcgactgga
     3061 tttggaaata cattaggtag tataggtatt gtaattggac ttggaataat atttggaaat
     3121 attctttcag aatcaagagc aacagaatct attgcaaagg ggttgttagc taagactgga
     3181 gaaaaaaaca gtgctttagc agtaactaca gcaggttttt taatttcaat tccagttttt
     3241 atggatgcag cttttgttat aatgatgcct ataattaagt atgtttcgag agtaactaag
     3301 aaatctctaa tggtttttgt atgtgcctta ggagttggga ctatagtagg tcatgcactt
     3361 gtaataccta caccaggacc tttagcagtt gcagcaaatg taaatgcaaa tgtaggtagt
     3421 tttatacttt attctataat tgttgctttt ccagcagttc ttataggtgg atggatttat
     3481 ggaaaacgtt ttgaaaaata tccagcttat gctattgatg agaatgatag agaaaaaaat
     3541 ttagaaaagg gaaaagatag cataaaaatt gaagatgatg attcttctaa agtaccaggt
     3601 tttggaattt ctatgttttc tttattgttc ccaatacttc ttatacttat atcaaatgta
     3661 tttagtatgt tcttagaaaa aggttctaca atgagtggag tacttgcatt tataggagat
     3721 aaaaacatag caatacttct tggaatattg gtggcaatag gatttttaaa gagatacata
     3781 aataaaccta tgggagatgt agttattgaa gctgctgatt cagcagggct aatattattg
     3841 ataacaggtt caggtggtgc gtttggcagt gttataaatg caagtggtat aggtaacttc
     3901 ttagttgata ctatgtctgg tcttagtata tcagttgtag tgcttggatt cttattaagt
     3961 gctttgctta gaatttctca aggttctgct actgtagcct tagtaacaac atcatcaatt
     4021 ttaggaccta caattttggc aactggaatg tcaccagttt tagtagggct tgctatatgt
     4081 gctggaggag ttggtttctc ccttccaaat gattctggat tctgggtact ttcaagattt
     4141 agtggattat cagttaaaga tactttaaac tcttggacaa ttggaggaac cattgctgga
     4201 gttgtagcat ttataatggt tttattgctt agtgttataa atggaattat accacttcca
     4261 ggtctataaa taacaaacta ttgtttatat tatataaatt acatttagac tatacaaatt
     4321 acataaaggg gcgttttaaa tgagagaagt aaaaccaatt agcatagata tacttaatac
     4381 ttttaaacaa gtagatgagg acagattaaa tgaacttctt gcagatgagc ttaagcattt
     4441 agatagaaaa attgttgtat tagatgatga cccaacaggt gtacaaacag ttcatgatat
     4501 ttctgtttat actgattggg ataaggatag tatggaacaa ggttttaatg agaaaaatag
     4561 tatgtttttt atacttacta attcaagggg atttactgtt gctcaaacta caaaagcaca
     4621 taaagaaatt tcaaagaata ttgtggatgt atcaaagaaa gttaataagg attttataat
     4681 cataagtcgt agtgattcta ctatgagagg acattatcct gttgaaacta atttattaaa
     4741 aagcgaagta gagagattat cggggaagtt atttcagggt gaaataataa tgccattttt
     4801 taaagaaggt ggtaggttta ctatagataa tgtacattat gttaaagaag gagaaatact
     4861 tgtaccagca ggaatgactg aatttgcaaa ggataaatct tttggatata aatcatctga
     4921 tataggtgag tggtgtgaag aaaagacaca tggaaagtac aaatctagtg atatgatata
     4981 tatttcttct aaagaactta gagagctaaa tattgaagct ataacagaaa aacttaaaaa
     5041 agctgaagga ttcaataaaa ttattgtaaa tgcaaccgat tatgtagatg taaaggtatt
     5101 tacaattgcg tttatacgag cagtaaatag tggtaaggaa tttatgttta gaagcgctgc
     5161 tgccataaca aaagtacttg gcggagtgag tgataaggaa atgcttacta aggatgagtt
     5221 agtatcaaaa ggtaatacca atggaggaat tatacttgtt ggttcacatg tgaataagac
     5281 tacccaacag ttagaagaac ttaaaaattg taaatatcca atagaattta tagaatttaa
     5341 tcaacattta gttcttcagg aaaatggtct aaaaaatgag gtaaaaagag ttataaaagt
     5401 tgtagaggaa aaaataagca gtggtaaaac tgttgctgtt tatactagac gagagcgttt
     5461 tgacttggat acgaatgata aagataaaca gcttatgatt tctgtagaaa tatcagatgc
     5521 agttaccagt ataattggta tgcttaatgt aagaccaaat tttattatag ctaagggtgg
     5581 aattacttct agtgatgttg gtactaaagc actaagagta aaaaaagcta cagtaatggg
     5641 acagataaaa cctggtatac cagtatggat gacaggcaaa gaaagcaaat ttcctaatat
     5701 gccatatata attttccctg gaaatgtggg agagattagc acacttagag aatcagttga
     5761 gatattgatg gggtagtata ttagtgtatt gaagtttaaa cgcttaaatt tactgtgtta
     5821 aaagtaaaat aaactaaaat tataaattaa gattaaaaat aatagtatat taattagggt
     5881 ttagagtatt ttacaaaaaa tattctaaac cttaatctta tgtattagta tttttattat
     5941 ttaactta
//
LOCUS       DAEPOZ010000219         5931 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.219, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000219 DAEPOZ010000000
VERSION     DAEPOZ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.219"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            132..1496
                     /locus_tag="KR440_003105"
     CDS             132..1496
                     /locus_tag="KR440_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBH0700115.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSNFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVENTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNPC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            1635..2801
                     /locus_tag="KR440_003106"
     CDS             1635..2801
                     /locus_tag="KR440_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBH0700116.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            2918..3700
                     /locus_tag="KR440_003107"
     CDS             2918..3700
                     /locus_tag="KR440_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBH0700117.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYAESLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(3819..4472)
                     /locus_tag="KR440_003108"
     CDS             complement(3819..4472)
                     /locus_tag="KR440_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0700118.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(4496..5860)
                     /locus_tag="KR440_003109"
     CDS             complement(4496..5860)
                     /locus_tag="KR440_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBH0700119.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
BASE COUNT         2233 a          749 c          903 g         2046 t
ORIGIN      
        1 ggcttttttt attggaagat ttatgttata ttgagatgaa tttattatga tataaaaaat
       61 aatgttagga taaatttaaa tctttttcta aaaatgtatt aatataattc ttaaagaaag
      121 gatttttaac aatggatata aaagagttag ttaaaatgca aagaaaatat tataatacag
      181 gtaaaacaag agatatatcg tttcgtatag aacagttgaa gaaacttaag ttagttgtat
      241 cacaaaatga agaaaaaata cttttagcac taaagaaaga tttaaataaa tcgaattttg
      301 aaggttttat gacagaggta ggcatgtttt atagtgaatt aaactttgct ataaagaata
      361 taagaaaatg gagcaaaata aaaagagtta aatcaagtat ggttaatttt ccatctatat
      421 ccaaagttgt tccacaacct tatggtgtta ctctcataat gtcaccttgg aattatccct
      481 ttcaattagc acttatacca ttagtttggt cattagcagc agggaattgt gttatattaa
      541 aaccttctga atattctgtg tcaacatcta gtgtagttaa agacatagtt gaaaatactt
      601 tttcaaaaga atatgttgca gttgttcaag gaagtcaaga agaaagtgaa aagttactat
      661 tggaaagatt tgattatata ttttttacag gaagtactaa tgttggtaaa ataatcatga
      721 aatcagcatc agagcattta actcctatta ctttagaact tggaggaaaa agtccttgta
      781 tcattttaaa agatgctaat atagacctta ctgccaaaag acttacttgg ggaaaactaa
      841 taaatgcagg acaaacctgc gtggctccag attatgtttt agtgcatgaa gatagaaaaa
      901 atgagttaat agagaaaata aaatattata ctaataagta ttttggagat aatccatgta
      961 ataatgagca atttcctaaa atcataaatc agagacattt taatcggata ttatctctta
     1021 ttgataaaga taagatagtc tatggtggaa attataataa ggagacatta aagatagagc
     1081 caactatagt agacaatgtt aattgggatg ataatataat gaaggaagaa atatttgggc
     1141 ctatatttcc cattttaaca tataaagatt tagatgaagt aatacaaaaa attatacaaa
     1201 tgccaaatcc attggcactt tatattttta caaaaaataa gtacctagaa aataaacttt
     1261 tagaaatgat tccagcaggc ggatgttgta tcaatgatac agttactcat atagctacga
     1321 attacttacc ttttggtggt attggtgaaa gtggtatggg ctcatatcat ggaaaagctg
     1381 gattcgatac atttactcac tataagagtg tgttaaaaaa attaaatttg gatgtcccta
     1441 ttagatatgc tccatacgat aataaattaa ttaaggttct taaaaagata atgtaggtta
     1501 tattgtagaa taataaatat aaataaaatt gagtatttgt attctgaata tattttattt
     1561 ttaatttggt atatttgtta taaaagtggt gattttgtga taaaatttaa ttatatagaa
     1621 ataaggagga aaaaatgaaa attaattgga atacaaaata cacaacaata gcaatttata
     1681 catttataat agctgctagt agtattatat tttatctagt atcatcacaa attgatgtat
     1741 tttctaataa tttagatgct atatttacaa cattacaacc ttttataata ggatttgcta
     1801 ttgcatatct tcttaatttt attttaaagt tttatgaaga cagaattttt ataaaaagtg
     1861 agaaattaaa aaaactaaaa caaagttcaa aaagaggctt gggtctttta ttaacatatg
     1921 caacagctac attgatactt tacttattta tgcatttcgt attgccacaa gtaattgaga
     1981 gtattgttgg actagcaaat gatataccta tgtatgtaaa caatgcaact aagcttattg
     2041 ataaattaat gactgattta aatcttgatg agcaatactt taatttggca gttgataagt
     2101 ggaatgagtt tgttacatat attataaagt ttgtaacaga tttaatccca atattaggaa
     2161 gtatgttaaa gaatgtagcc tctagtatat ggaatattgt actaggatta attgtttcag
     2221 tatacttatt aattgataaa gaaaaattct atggattaag caaaaaaata acttatgcag
     2281 tatttacaga aaaacaagct gccagaattt tagaattaac tcatagaagt aactatacat
     2341 ttggaagatt tttaggtggc aaaatattag attcattcat tataggtatt ctaacatttg
     2401 taatacttac attagttaaa atgccatata cattattaat atcagttata attggtatta
     2461 caaatattat acctttcttt ggacctttat ttggagctat accatctact ctaatagtga
     2521 tgtttgtatc tcctataaaa gctttctggt tattgttgat tatattaata atacaacaga
     2581 tagatggaaa tattatagga cctaaaatac ttggagattc aattggtata tctgcatttt
     2641 ggattttgtt ctccttattg gttgcaggaa aattattagg ttttattgga atggttatag
     2701 gagttccaat gtttgcagta atttattcta taattaagga tattgttgaa agtaaattgg
     2761 acaaaaaggg attaccgaca gatacttccg attacatgta gctaaaaaat attatgtata
     2821 attaaaaata tataaattaa gcaatattta aagcactgag aaagttaaac tcttggtgct
     2881 ttttagatat aaaattatta tagaagaggt gttttttgtg gcatttatag aatgtaaatt
     2941 taaatctaaa tatactggag gagaaactga tataattgta attttgcctg tagcaaatgg
     3001 aaaagaactt tttagtggta aagagatata tgattttgaa ggtaaattta aaacattata
     3061 cttatttcat ggtttagaag atgaccaaag ctcatggatt agatatacta atgtagagca
     3121 ttatgctaaa aataaaaata tagccctagt aataccaaga gtagaaacta gtttttatac
     3181 agatatggcg tatggacacc aatattttac ttttgtaagc gaggaattac ctaaatttgt
     3241 tagagctata tttcctttat cagataaaag ggaagataac tttgtagctg gtatgtctat
     3301 ggggggatat ggagctttaa aattagcact ttcaaaacca aaagagtttt cggcagttgc
     3361 aagtttttct ggttcgcctg atatgattca tgagttagaa aatcaacaaa tagaaaccaa
     3421 tgcagacatt ttatttcacg attttggtag accagaagac gctagaaata gtgagaatga
     3481 tttactttac atacttaaga accttaaaga aagaaatgaa gatattccta aaatttatca
     3541 attttgtggt gatagagatt ttttatataa aaataatcag atgtttaaaa aatatgcaga
     3601 gtctctgggt gtagatgttg aatatgaaga gtgtgtaggg gggcatgaat ggcgtctttg
     3661 gaatttatgg ataaagaaat ttatagatat aattgggtag tacacttatg gtattaacag
     3721 gtatactata aaatatcttt aaatatgata aagactgaaa tcctaaaatc aaattatgat
     3781 tttaaaattt cagtctataa gaatttaata aaattttatt aagttcttac acgagttatt
     3841 attggagtaa aattgtcaac tatatctata tagtatttac tcaattcctc taaaatctct
     3901 tcgcataaat ccgtactatg accaagatta ataagaaact gttttgtaaa atgctttatt
     3961 acagcttgag aagatatatc gtacttgtgt gaagtataaa ctctaattat ttcaggtttc
     4021 atagcaagta taccaataca agaaagtatt aattcatcat gttctataga ttctttatca
     4081 ttaacatatc ttttcatgac catttcggtt acacttcttt gatagcttaa aatagcttct
     4141 tcataagcta tatccaaata aaaatttcta tatgcttcgt tttttagaag gaaacaaagg
     4201 tcagcattca tagcaagaaa gaacatcatc atagtacctt cttcatggta gttatagatt
     4261 aaagactgca tttcatttct tatatcttca tatattatag ctcctatttc gcttttactt
     4321 gagaagtaat agcttattag acctaaattt atattagctc tttctgcaat ttgcctagtt
     4381 gttgtcttat gataaccttg ctcaaaaaat aattctctag cagcacttat aatactagct
     4441 tttgtttctt tgcccttttc tgattttgac atatttactg tgtatacctc tcttttcaat
     4501 ctttttttga aaatttgcta actataaata ttgtaataga accacctatt ataggaacca
     4561 aaattctgtc aaatacagta agaataccaa tcatatttaa taatgccaat ataacactta
     4621 ctataacagc atagatagct cctttttttg tagcgccttt ccagcaaaga ccaattacaa
     4681 atggtgcaaa tattacacct gtacataatg gagccggcca aaacaataat tctaataaac
     4741 tatcaaattt aagtgctatg attgcaccta ttatagctgt caaagctgat gcaatttgag
     4801 aatactttag agtatttttt aaatcattaa attctatatt aggatgtaat aatttgttat
     4861 aaatatctct tgatataata gcactcattg aggtcattaa tgaatcggca gtagacatta
     4921 tggcagccaa tatagcagct aagaataata cagatattaa aggtggcaaa gtagtcgata
     4981 tcatttgagg taaaacctga acagatgata tgtttggaaa taaactacca ccaatagctc
     5041 caattattcc tatagggaag gcaagaaata atagtataaa tcctgcaatt acactacttt
     5101 taactgcatt tttagcatta tcagcacatt gatatcttaa aaaactttca taagaaaata
     5161 ttgagcctaa aactaatggt aaaactgtat acataaaatc actaatcgat ttatttcctg
     5221 gaaagaatgg ttcttttacc attgattgta acataagtaa agggttgtcg ccaactaatt
     5281 ttaagcttat ataagctact attggcaaaa acactataca aatagcaagt tgaataaatg
     5341 ctgatactgc aactccccac attcctgatg tataagtata tataattata actataactc
     5401 ctataattac agctatatca taaggaatac caaatgcagt agatacagaa ccaaaagaaa
     5461 ttaattgact acccataata gctagtatag atattggtgt tacaagactt tgataagctc
     5521 ttaaaaatac actatgacca tatctagttt caatataatc agcaggtgtt atatcacaag
     5581 ataagcttct taattttcct gcgaaaaagg catttataaa gcaagctaaa gctgcaccta
     5641 tagtagaata cattccaacc caaagtcctc tttcagctcc ccattgaact ccaccaacta
     5701 agcaccctcc tccaaaagat gtagcagcaa tggttgcagc tagtataggc aaaggaagat
     5761 ttctacctgc gattaggaaa tcggatgctg ttttaacttt ttttgaaaaa taaaaaccaa
     5821 tccatatgta tattgcacac gtaacaatta aaagtaacat agtcttttct cctttataaa
     5881 atattttaat aaaatatata aaatatattt aaaatatatt ttatataaaa g
//
LOCUS       DAEPOZ010000220         5877 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.220, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000220 DAEPOZ010000000
VERSION     DAEPOZ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.220"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            354..1181
                     /locus_tag="KR440_003110"
     CDS             354..1181
                     /locus_tag="KR440_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBH0700120.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGNGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
     gene            2198..2662
                     /locus_tag="KR440_003111"
     CDS             2198..2662
                     /locus_tag="KR440_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0700121.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKKNFSLS
                     KEDKRKNFISFINDNKELIGFINLLDEGSYVFFGIGIKPDYCGMGIGKEIISLGLQEC
                     RNKYSTKPIVLNVRTWNMRAVKCYKSQGFKIVETQVQKTHLGDGEFFVMRYQ"
     gene            complement(2929..3573)
                     /locus_tag="KR440_003112"
     CDS             complement(2929..3573)
                     /locus_tag="KR440_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0700122.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMIPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKSLSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPIKRKIR"
     gene            complement(3503..4792)
                     /locus_tag="KR440_003113"
     CDS             complement(3503..4792)
                     /locus_tag="KR440_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0700123.1"
                     /translation="MNLFIKECKNILKSMVYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIDEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINLIWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFIMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            complement(4789..5661)
                     /locus_tag="KR440_003114"
     CDS             complement(4789..5661)
                     /locus_tag="KR440_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0700124.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILDSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
BASE COUNT         2113 a          812 c          707 g         2245 t
ORIGIN      
        1 attacatatt ataaattttc tatcttctat cttctatctt ctatcttcta tcttctatct
       61 tctatcttct atcttctatc ttctatcttc tatcttctat cttctatctt ctaaataatt
      121 ttaacatgcc cataaaatat aatacccaca aatgataaat aatactcaaa atatctattg
      181 aaataactat acatccttaa ataacttaac ttaaatctaa acatttcaaa aacttcttaa
      241 aaatatgtaa aaatttgatg ataaagtagc gattttttca aaaaaaatgt aatatagagt
      301 tatatcttat aatattttag agataaattt tatcatagtt gggagaaatt tgtatggatt
      361 taagcaaaaa agtaaaaatt ttagaaaatg catcagatgg caaaagcgaa tttgaaatat
      421 tagaatttga aaaacttgag ggagcttcta gcccattaat ggcaatggga ttatattttg
      481 ctagagaagc tggtttgaaa gctagacaaa ttaagataaa attaaataat tcatcaataa
      541 aaacagaagc tggtgcatta tactattata aaggaaatat atcttctgaa actaagattg
      601 gtggtgttgg aggtcttttt aaaaaaggaa tatcaggagc acttactaat gaatcaatga
      661 tgaaacctgt atacaaaggt actggagaag tttatttgga accttctttt agacattatt
      721 tatttatgag tcttgataat gattctataa ttgttgataa aggattattc tattgttgct
      781 ctgatacaat agaagtaaaa gcatttacac aaaagaactt ctcatctgct ttacttggtg
      841 gtgaaggctt cttccaaata gaattaagtg gtactggtgt aatcgttctg gaatgtgttg
      901 tacctcaaag tgaaattgta gagtatgaat tgaaaaatgg agaagaatta aaggtagatg
      961 gtaactttgc aatagcaaga acatctgggg ttaatttttc tgttacaaaa tcagataaat
     1021 ctttaattgg ttctgcttta aatggtgaag gtttcttaaa tacatttact ggtaatggta
     1081 cagtttggct agctccaact caagtaatgt atgaaaaact aagttttgga gcaatgccta
     1141 ctaatgataa tatgaataat aaagtttcta agcaaaaata gttgttttat aaatatttat
     1201 taaatcatta ttgattttat aaagtcacta taaattttat tagattatta ttataaaatc
     1261 agagtgtgga aatgtttatg tgtattcttt aaataaaaat ttccacattc tgtaaggtta
     1321 ttatacttga tatataaaat tcaatttagg aacatagtct aacatttata aacatattaa
     1381 ggtaatgatt tctcttggta attattctca tatcttattc tttgattata tttatcttat
     1441 tcctagccct tatgtcatat tatatttctt atcttattat ttataattag cattaactta
     1501 tctaaaatct tgtatttttt taatagtaaa gtttacatct atattaaaaa gtttacatct
     1561 atattaattt cataaaacaa tcaaggtagt caagagattc aactttattt gattttaaaa
     1621 atttaagtga atttgttctt atacacatct ttttaaaatt atatctgtag aaaatatcat
     1681 ttagagctat aattaaatta ttaaatttta gaaaatttat taaaaaatat aagttaaatt
     1741 aatattttta aagattcttt tttataacta ctagaatact aatttagtag ttattgtatc
     1801 tctaaagttt aagcatggat agatatacat ttgataattt gcctataatt tacctactac
     1861 ttggagtatt ggagcaaata caatatatta ataataagtt cctaaaacca ataaattaac
     1921 taatacacat aaagatattg atgttgatta tacaactata agaaacagat ttaacagaat
     1981 ttaatcaagc taagaaatta catatatcgt gatgtttttg aattagaagg taattcaaaa
     2041 ttagtttata gtcttctgtg aatccaataa aaagtacagg gtacaagaat tgttagtaaa
     2101 atttttaaag attttatgga tagatataaa tagttttact tattttgtaa aatttaaaat
     2161 accattaatt accttaaata ttttaatagg aggacatatg aatttaaata aagttaaatt
     2221 tactgaagaa catgctattg aagttactaa ttggaaatat gaaggtaaat attcaatata
     2281 caacctacct ccatgggagg aaattaaaaa gaagaatttt tcactatcaa aagaagataa
     2341 aagaaaaaat tttataagtt ttataaatga caataaagaa ttgataggtt ttataaactt
     2401 attggatgaa ggaagttatg tattctttgg tattggtata aaaccagatt attgtggaat
     2461 gggaattggc aaagaaataa ttagcctagg attacaagaa tgtagaaata aatatagtac
     2521 taaaccaatt gtattaaatg taagaacctg gaacatgaga gctgtaaaat gttataagtc
     2581 tcaaggattt aaaatagttg agactcaggt acagaaaacg catttagggg atggagaatt
     2641 ttttgttatg aggtatcaat agtttataac actagtcata ttgctaatat gacaattaaa
     2701 atgaaaaaat acatgtgtgt atgtcatagc aataagtgtc tttgaaatat aaatttgaga
     2761 gcctttgata aaattcggta tacatatata ttttatacta cagatatata aatctatcta
     2821 catacataaa gagattgtaa gtaggtttgt gtatataaaa ataaatttat atttcataaa
     2881 tgtacttttt atttgtatta cctttatttt atctaagctc tcattttatt acctaatctt
     2941 cctttttatt ggttttatta agagagatgc aattatcaac acaacacttc caagtatcag
     3001 ccaatatttc tcatcaaaac taccttgact catagacatt ataaaaaatt ttccaacata
     3061 cttgccttta gtcatcatat ttaaaattaa atatcctata gatatcatgt aacctatgat
     3121 tgtattatta aataaatttg agaaaaacat tcctaaactt cctataaaca tagctgatgc
     3181 gaaacttcca ataatatact caactactgg aaaattacat ccaagatata ttaaaacatt
     3241 tacaaaacct atgattaata ccactatacc tataattgac atcagtatcc taattaattg
     3301 tacaataact tgagataatg attttgaaga tactatctca tcaatttctt ttgattgctc
     3361 tagcgaaaat acaggtacca acattactat ccctattatt gcaacataat tttctaaaca
     3421 tatagctgtt gaaattctgt ctaaattagc tactccttgt attagaggaa tcatacatag
     3481 caatataatt gaacatatta aattagttaa agtgcttaat ttgaagtttt cttttattag
     3541 aactaaacag ttttgaaaac tttgcccaaa catcaagatt cccccttctt ttttgctcat
     3601 ataagaatat agctaacaaa acaagcacca atgctcccaa gcttatcaca attctattaa
     3661 tcataatttg attgaagttt tgtataataa tatccccatt taatacacta ttatgtctta
     3721 tcattagccc cattgttgaa taatcacctt tgagtcctgt agaatttata ctgtaaagcc
     3781 accaaattaa ttgcacaata attgctactg gtgtatctgt taatatagta atactcattc
     3841 ctacagctgt aactatcata agagttggta gtatccacca taatgtatat tttataaatg
     3901 ctaagttatc aatagatata cctttatctt tagcaaagaa aatcaaagga agtaaatctt
     3961 tcatacctaa aattaataca ggcaatatca ttataaagac aagtgcaaaa tatcttgata
     4021 aaactatttt gcatgaagat acttgttttg aataaataat ctctctcatt cttgaacgct
     4081 tatctttaag tgacataaaa actgccacaa atactggaaa tatacttaaa actataccta
     4141 tataatcgca aaaaattctt gcatacccat tactaacttt atctttttgt attgtatagt
     4201 tgtaattctt catagcttca tcataggtaa ctggaactac tccaaatctt tgtaacttgt
     4261 caaaagcata tgcagaccct cctcctaaaa tgtcgtctac tctttgcatt atttcttcaa
     4321 atctttcaag agatacttta gaataatcta tttttatact ttcatcagtt ttccaaatta
     4381 aatttatttc gctaatactt ttaccagtta tttcttctat atatttttct atggtcttcg
     4441 ttttattttg gtctaatttt atatacttaa ctacccctgt tggatacgtt gtatatgtgt
     4501 ttttttgata ttctctaatt aggtttatag ttgcatttgg aatgaccttt tctgttttca
     4561 tagattcttt ttgaccatat ggatattgat tgtgtacaga gtaatattcg tcaatattct
     4621 ctactttttg tggagcaact aatggatttc catcattagg aatattttta cttacatcat
     4681 ttgaaactga acttccaagt tgagaaatat agaaaaaaac taaaactaca ataaaaatat
     4741 aatataccat actctttaat atatttttac attcttttat aaatagattc atatattatc
     4801 tcctcccatt ataagcatat agccatcttc aatagttgga ttacaattta ctgctgtctc
     4861 aaaaggttta tcatctgata taatcctaag tatagccttt ccaccatgag tcaacattcc
     4921 tataactata aatctacttt ttatattttc aatgtctttt ttatctactt caatactata
     4981 tactttccct tcagctagtt ttgctaatgc ttctgttgag ccattgtata tgacttttcc
     5041 actatccaat atagctacgt tttcacatgt agcttctata tctccaacaa tatgtgtaga
     5101 taaaataacg attctatcat ttgcaatttc gcatagtaaa tttctaaatc ttatacgttc
     5161 ttctgggtca agacctgctg tcggctcatc cactattaaa actctgggat tatgaattaa
     5221 tgcttgtgct atcccaagcc ttctattcat acctccagat aaagatttta ctttcacatt
     5281 ttgataatta gtaaggttta ctttgcttaa taactttgct ataatttttc ttctttttct
     5341 tagtggtata tccgataaaa ctgctaaata atccattgct ttatatactg tcatgttagg
     5401 atacattgaa aaatcttgtg gcaaataacc aaccatattt ctaatttcct tagtattctc
     5461 aataggaact ccacacatat gtattgaacc ttctgttttt gataataaag tggctaaaat
     5521 tctcataaat gtggtttttc ctgctccatt tcttcctaaa agaccaaaca taccactgtc
     5581 gatttcaaga tttatattat caagagcttg ttttttccca aatcttttac taagattgga
     5641 tactgctatt ttcatttcca tatataaata cacccccaga aataatttta tagaatttta
     5701 tttttataaa tttttactgt attttcataa taaattctaa aacttaagat attatttata
     5761 aatttctaaa ttttttctaa agattttaat ttttctgttt ttaattaaat taaagtatct
     5821 ttgtttctga aattttttaa gtttatgaag ctataattta atatatattt attttat
//
LOCUS       DAEPOZ010000221         5851 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.221, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000221 DAEPOZ010000000
VERSION     DAEPOZ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.221"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            1042..2073
                     /locus_tag="KR440_003115"
     CDS             1042..2073
                     /locus_tag="KR440_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBH0700125.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFT
                     LDGDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALLMKVADVVRDCL
                     DIEDNNEKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
     gene            2206..3504
                     /locus_tag="KR440_003116"
     CDS             2206..3504
                     /locus_tag="KR440_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBH0700126.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLPHFNKVITTTPNNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(3618..3863)
                     /locus_tag="KR440_003117"
     CDS             complement(3618..3863)
                     /locus_tag="KR440_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBH0700127.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            4014..4880
                     /locus_tag="KR440_003118"
     CDS             4014..4880
                     /locus_tag="KR440_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HBH0700128.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(4884..5420)
                     /locus_tag="KR440_003119"
     CDS             complement(4884..5420)
                     /locus_tag="KR440_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HBH0700129.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(5610..5813)
                     /locus_tag="KR440_003120"
     CDS             complement(5610..5813)
                     /locus_tag="KR440_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBH0700130.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
BASE COUNT         2312 a          602 c          893 g         2044 t
ORIGIN      
        1 aaaaaggtaa ttgaaaatat cgtaaaaaag atttaatgtc atcacagggc tattgattaa
       61 aactgtctta atatataaaa tgttatttat attaagacag ttttattatg caaaatatca
      121 gtttatagac ttttaatatt gtttaatatg tatatgtaga gtttccatta cagtagcatg
      181 attattaatt agtagcctga ttattaatat actattattg atttttatat gcattatatg
      241 tatttttaga atatgattac ctttttaaaa taatgactat ttataaaact ctatagatat
      301 ttgtttcatt tagctatgca tactttaatt aattatatta atatttaatg gagtataata
      361 taaatattaa agcgacatct tatagtttat tgaacctata gtaatgaatt aataaatggt
      421 ttaattctat gaaaagattt ccttttatgc aatttattta ttgaaatatg atataatata
      481 taaaggtgta agtattagtt gttaaaataa tatttatatt agtttttatg catacttggt
      541 ataagttttt catatattgg ttaaaattga tgttgattta ttatttttag tattttttca
      601 ccttttgatt catactttgt agaatggtgt attttataaa actgattata agacaagttc
      661 attcaaatta gcatttttat atcatataaa taattgttcc ccctaaataa gatttagatg
      721 tatagtatta aaatcaaaaa gtataaaata aaattgatgc ttaatcatag ataaatatta
      781 ataaaatata caaggggatt gtctaataaa aagataatct ccttttttac aaataatcct
      841 aaattgtgat ataaatctaa aaattttatc ttaacattta accatttaat gataataatt
      901 gatataataa atataaatta aatatatggt gtacttattt tatgttttta gcaagaaata
      961 tagcatttaa aagataaatt gtgcaactta aatcaaagtt taagatgaac tattaacata
     1021 gatatattaa aaacttttgt aatggagaat atatgtgata tgaataataa agttaaggtt
     1081 gcagtattag atacaggaat agacaaagaa catgattact taaaagataa tttagttgga
     1141 ggaattgctt ttgaatgtat gcatgattat atctttatat cagataaatt tgatgatgaa
     1201 gatggtcatg gaactgcttg tgcatcaata ataaagaaag agtatgaaga tgtagagtta
     1261 tttgtaataa aaattttgag aaaagatggt attacaaata taaaagttct tgaagaagca
     1321 ttaagatacc ttttagacac taacataaga ttaattaatt taagtttatc agtcatagga
     1381 gtagagagtg ttaagggctt atttgaagtt tgttacgaat tgtttagaaa aggaaaaata
     1441 atagtatgtt cactcgctaa tgattttgat ttaagttatc cagctatgtt taacaatgtt
     1501 ataggtgtaa gaggatttac tttagatgga gatgattcat tttggtacaa caaaaaatat
     1561 gatgttcaat gtgttatgga tagcaattca tatataagtt gtgatataaa taattcctat
     1621 agattaccac caaaatgtaa tagttattta tcagcaaagc ttactggtaa aatagctaag
     1681 atattatcag aaaaaccagg cataacaata tctgatttaa atgataagct tgaatcatta
     1741 gcaactagaa atcattggga tagttgtgat tttgatgagt gtagtagaat tgtaaacttt
     1801 aaattggatt tgtatgacaa agaaaatgct cttcttatga aagttgctga cgttgttagg
     1861 gattgtttag atattgaaga taataatgaa aaactcttta agggtagcct ttttaataag
     1921 gagataggtt tggtttatga taattgtttt aatctgttga aaaagctaga gcatagattt
     1981 gacataaagt ttaattatat ggacatctca aaatatgact tagtttcaat atatacactg
     2041 actgaattag tggaaagata taaaaactta taaataaaga tttgtagcta aatttaatgt
     2101 agaaggtatt aaattatatg tattctttgt cggtagatta aaagtaaaaa atatactata
     2161 attagatagc ataagagaaa atgccaatac agggggagtt agataatgaa atatgaagaa
     2221 gcattagagt acatatcaca aactaataaa ttcggtataa gattaggatt agaaaatatc
     2281 ggaaagttat tagaattatt aggaaatcca caagaaactt tgaatataat acatgtagct
     2341 ggaactaatg gaaaaggttc tgtgtgttct tttgtttcta atatactaag agagtgtgga
     2401 tataaagttg gtttatatac atctccatat ttagaaactt ttactgagcg tataagagta
     2461 aatggacaaa atataccaca ggatgatgtt gctagaataa ttgagttaat aaaagaaaaa
     2521 atagagatta tggtaaaaga aggctatgca tatccaacag aatttgaagt ggttactgca
     2581 atggcatttt attactattc agaacaaaag gtagactttg tggcattaga agttggttta
     2641 ggtggcagat atgatgctac aaatataata acaaaatcac ttgtaagtgt gatagtatct
     2701 ataagtttgg accatacagg tatacttgga gatacaatag aaaaaatagc gtacgaaaag
     2761 gctggaataa taaaggaaaa tggagtagta ctagtatatg accagactga tgaagcgaag
     2821 gatgttataa agtcagtttg taaagagaaa aaagctaaat atatagaagt tgattttgat
     2881 gatataaaca taaaaaaatc agatataaat tctcaaatat acgattgtac tgtaatgaaa
     2941 gaaacttata gagaccttga aataaaattg ataggtgaac atcaaataaa caattctatc
     3001 ttggcaatta gtgtaataaa atatctaaaa gacataaata aattagcaaa tataaatgaa
     3061 gaatctataa gaaaaggact tataaatact aaatggccag gaagaataga aaagataaaa
     3121 gaaaatccaa tttttataat agatggtgca cataatgaag atggagcaaa atctttggca
     3181 aaagcacttg ataagaattt taaaggtaga aaattaacct tattaatagg tatgttagaa
     3241 gataaagata ttgatagtgt tttagagata ttgttgccac attttaataa ggtaataaca
     3301 acaacaccaa ataacccaag ggccataaat tcagatatat taagagaaaa agtattaaaa
     3361 tatgtagatg atgtaacatc aaagcatgag atagaggatg cagtaaatta tacattagaa
     3421 acatcaagtg aggatgatat tataataagt gcaggttcat tatatatgat tggtacagtg
     3481 agaactttag taaagaaatt ataattttat aaaataaata tacaaataaa gatataaaaa
     3541 agagttgtct aaaaaatgga caactctttt tatataaagt aagtttttaa taattttact
     3601 gatgtttata taagtaatta tatagattga cgtatagcct tagataattg gtctgggcaa
     3661 gaagtacctt ttccaccaca gtcaataccg tttaatttat cagcaacttc aagagcattt
     3721 tggccgatta ttaagcttct aagtcctgat aaattaccat tacatcctcc tataaactca
     3781 gcatcaacaa tcacattatt ttcatcaact tcaaaaatca tttctctaca acatacacca
     3841 cttggattaa atgttacttt cattaaaaag acctccatta aatattattt ctttttttaa
     3901 caataataat aataatacca aatatatgcg taataatgca actataaagc ataatacaag
     3961 ctaatgaaat aatatattat tgtagtatat gtattgtaga gaggaggggt tatatgaaaa
     4021 tctatatttc taactactta tcaaagagta ttagcatagt tgattattct acacttgaac
     4081 tagaaaaaga aatagtatta gaagataata tatatccaca ccatttttgc atagaaaaag
     4141 aaaaaaattt aatatacata cctagttcaa gtaatgggat actttatgtc ttagatttaa
     4201 gtaatgataa aataattgat actgtctcta taggaggaag tcttagtcaa gttgtactta
     4261 gtgataatga attgtttgtg gcaaatgaag attctaatag tatatatgta ctagataaaa
     4321 acacattaaa tccaatagga attattggag ttgataatat gccacatgga tttgactttg
     4381 atagagaaag taaaaaatta tatgtaccgt gtataaactc gatagtatgt atagatacaa
     4441 ttaataaaaa tatacaaaag aaaataaata tggactttag agcttggcat atagagcttg
     4501 ataaacaaaa gaaagaaatg tatatatcaa cacttgatgg aaagcttata ataatagatg
     4561 aagtaagcat ggatataaaa aaggtattat atgaattttt attaccagta gaaataagat
     4621 ttaattatag tggcaaaaaa gtttatgttg ctgatttggg ttataataat gtaagaattt
     4681 tagattatac aacgagtaga tacattggaa atattgaaat aaatgggatt cctcaaggct
     4741 tagaaatatc taaagatgaa aagctactat ttgtatcaga tacacaagaa aattcagtaa
     4801 aagtttatga aactgcaaac aataaattaa taaaagaaat caaggtagga aaagagccta
     4861 caactataat ttgtttgtag gctttaatgt atgtctctta taagtaaatc gataacttcg
     4921 gcatacatat aagatgcatt atttttccag ttattgcaaa taagttttgc ttgtttctct
     4981 gatgaaacat ttagatttaa atctattaag ttagactgat tttctatgac tctaaggttg
     5041 acaataaatt cattatcact ttgttttaca aaactagact ttacttgagt atctgctagt
     5101 agattttctt tgttagaatc tatatacttg tctatttttt cagtaacact agtaggtatt
     5161 cgattaaaga agtattctag actttctacg cctctttgag taagagcata atattctctg
     5221 tcagagtctt tatatgtagt caaaaactta gattccataa gctgagataa aagctgttgt
     5281 aatgaaaaat aattcatcat ctcagtctct aatactactt gagttatttg agaattagtt
     5341 aaatccattt taattttttc taatatatat aaaattaaaa gtttgtgata ggctaattct
     5401 tcagacgagt tttcaaacat aaatatcatc ctccgtcaaa ctatttctat atataatata
     5461 ttaaactaat ttgtacaaaa ttaaaagaac aatttgtatt gtattattaa tctttaataa
     5521 ttcatataaa acaattttat aaataaatat agagaaacag ggctgctaat tagcagccct
     5581 gttgataccc aggtgtcaaa atttactatt tattttccag ccatttgtct ttcagccatt
     5641 tcaactaatt tcttagtcat atatccacca acatatccat tttctctagc tgttaagtta
     5701 cctttatcta tgttttcata gttagataaa cctatttcgt tagctatttc taatttcatt
     5761 tggtttaaag cagcttttgc ttctggaact actgttctgt ttgaatttga catagtaaat
     5821 ttcctcctca aataattagt ttatgatgtc t
//
LOCUS       DAEPOZ010000222         5827 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.222, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000222 DAEPOZ010000000
VERSION     DAEPOZ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5827
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.222"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            185..1636
                     /locus_tag="KR440_003121"
     CDS             185..1636
                     /locus_tag="KR440_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBH0700131.1"
                     /translation="MNFLNGILTSINNAVVFVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(1856..2317)
                     /locus_tag="KR440_003122"
     CDS             complement(1856..2317)
                     /locus_tag="KR440_003122"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBH0700132.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGRYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLN"
     gene            complement(2423..3616)
                     /locus_tag="KR440_003123"
     CDS             complement(2423..3616)
                     /locus_tag="KR440_003123"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HBH0700133.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(3639..4304)
                     /locus_tag="KR440_003124"
     CDS             complement(3639..4304)
                     /locus_tag="KR440_003124"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBH0700134.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSSNHKSNIDKDFLFK
                     NGF"
     gene            complement(4338..5441)
                     /gene="ribD"
                     /locus_tag="KR440_003125"
     CDS             complement(4338..5441)
                     /gene="ribD"
                     /locus_tag="KR440_003125"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBH0700135.1"
                     /translation="MNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKDSNIIGK
                     GYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKRVVI
                     STLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKSAVS
                     LDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQEKVS
                     HPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSKNNL
                     VDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPTFVS
                     GEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(5573..5694)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
BASE COUNT         1931 a          858 c          803 g         2235 t
ORIGIN      
        1 taaaaaaagg tttgatttga atataaaact gcaatatatt aaaaaaatta ttcattttaa
       61 accttgtaat ttgattttat aaatattata attaaattaa tgaattttta acaaggaatt
      121 attcattttt ttgattttaa gatttattga gaaaacaaaa ctaaataggg gggtaattaa
      181 tataatgaat tttttaaatg gtattttaac gagtattaac aatgcagtag tatttgtaaa
      241 caattactta tggtcgtata tcttaattat aatgttagtt gtaattggta tttactttac
      301 tattaaaaca aattttgtac aattcagata ttttatagaa atgtttagac ttctgggtga
      361 tggtacagcg aataaaaatg caaaaaagga aggaaaaatc tcttcatttc aagcattttg
      421 tataagtaca tcttcaagag ttggaactgg aaatatagct ggaatagcaa ttgcagtggt
      481 agcaggggga cctggtgctg tattttggat gtggcttata gcattaattg gttctgcatc
      541 aagctttgtt gagagtacat tagctcagat ttacaaagtg aaagatggtc aagcttttag
      601 aggtggacca gcgtattata tggaacaagg tctaaataaa aaatggatgg gtattttatt
      661 ctcaatactt ataactatat gttatggttt tgtatttaat gcagttcaag caaatactgt
      721 ttcacttgca tttaataatg catttggaat aagtaaaatg gctatgggaa taggtcttgg
      781 aattttaact gcaatagtta tttttggtgg agtgcataga gttgcaaaag tttctgaaat
      841 aatagttcct gtatttgcag gattatatat attagttgct ttggctatag ttgtaatgaa
      901 tataacagag ataccaagtg ttatagcatt gatagttgaa agtgcatttg attttaaagg
      961 aatggcgatt ggtacattta tgggagttgt aatgactgga gtgaagagag gtctattttc
     1021 taatgaagca ggtatgggta gtgctcctaa tgctgctgca acagcacatg taactcatcc
     1081 tgtaaaacaa ggtttaattc aatctttagg tgtttttaca gatactataa taatatgtag
     1141 ttgtacagca ttcatggtat tattatattc aggttatagt agtagtggag caactggaat
     1201 tgaattagct caagaagctt taactcatca tataggacca gttggaaata tatttatagc
     1261 agtttgtata ttcttgtttg catttagttc tatagttggt aattactatt atggtgaatc
     1321 aaatatggag tttatgagtg gaagtaagac taaacttaat gtatttagag tttttgttgt
     1381 aggcatggtt ttatttggtt cacttactca agtagatgta gtttggaatc ttgcagactt
     1441 atttatggca ttgatggcga taataaactt aatagcaata gcacttttag gaaaatatgc
     1501 atttattgct ttaaaagatt atacaagtca aaaaaaatca ggaataaaag accctgtgtt
     1561 tattgcagat gaaatagaag gcctagaaaa ggtatcagag tggcataggg aattatctga
     1621 ggaacattta ggataaaaaa tagtataaaa ttataaaacc attttgaaag actagagtat
     1681 gtatatgctt tatatgaata taatattgaa ttaatttata ttactggaat ttttaattcc
     1741 agtaatattt ttttattata taatttagct ttattaaaag tttttattat taaaaatagc
     1801 ttatttatcg taaagtgaat gatattggtt ttttaattat tatgaatgtt ggtatttaat
     1861 ttaaagactt aaataaattt gccatttcta gcgcattcat agcacaatca taacctttat
     1921 ttccagcttt tgtacctgct ctttcaatag cttgttcaat attttctgta gttacgattc
     1981 caaatataac aggtaattca ctgtctaaag atacttttgc tatcccttta gagacttcac
     2041 tacatacata atcataatga gaagtagccc ctcttataac acagcctaaa cagattatag
     2101 catcatacct accactttta gccatttttt gagcaacaag aggaatctca aaagctccag
     2161 gtacccaaac tatttctata ttttctggag aaacgtcatg tcttaaaaga cagtcttcag
     2221 ctcctgataa aagttttgat gttataaatt cattaaaacg agaatttatt atacctattt
     2281 ttaaatcttt tccaataagt tttccttcat atatcatttt tatttcctcc aattttattt
     2341 atgttataga tttatattta taaatatctt taattttaat tataaaaaac tacttagtag
     2401 aaaaatatca tcatataatc atttatataa tatctaaaag atgtcccatt ttatcttttt
     2461 tagtttttaa atatagctta ttaaagtcat taatttcttc attaataggg attctatttt
     2521 cgatttttat tccatatttc tctaattgat ttattttatc cgggttatta cttaaaagat
     2581 ttatactttt tatgtttaaa ttttttaata tttgagcagc catatagaaa tctctcatat
     2641 cttcttcaaa tccaagcatt aaatttgctt caacagtatc gtaaccttct tcttgaagtt
     2701 tataagcctt aattttattt aaaaggccaa tacctctacc ttcttgcttc atataaatta
     2761 aaactccact tccattttcc acaattttct tcatagaaga ttctagctgt aatccacaat
     2821 cacattttag agagtggaac acatctcctg ttaaacattc agaatgaagt ctaactagag
     2881 tattttctat atttatattt ccataagtaa gggcaatgtg ttcttcattt gaataagtat
     2941 ctctatatcc tatgatttca aaatttccat attttgttgg aaggaaagca ctagatactt
     3001 tttctatagt agtttcattt attttgcggt attctataag gtcttttata gtgatgattt
     3061 ttaaattatg cttttctttg aaaatcatta aatcatcaac tcttgccatt gtaccatcat
     3121 ctttcattat ttcacagata agaccaatct ctttaaaacc tgcaagtcta gcaaggtcta
     3181 ctgaggcttc tgtatgtcca tccctgacaa gaactccacc agtttttgct attaaaggaa
     3241 atatgtgacc aggcgttttg aaatctttac ttgtagaatt ttcatcacag aacaatttta
     3301 cagtttcagc tctttcatat gcagatatac cagttagagt atctgctcca tctatagtaa
     3361 cagtaaaggc tgtttgaaat gtatctgtat tttctctaac cataagaggt atattaagac
     3421 ttttaaattt tttttcagtg gcaggtagac aaattagacc tctaccatat gtagccataa
     3481 aatttattga ttcaggagta gccatttcag ctgccatcaa taaatctcct tcattctctc
     3541 tactttcatc atcaactacg attaccattt tcccattttt tatatcttca atagcttctt
     3601 caatagtatt aaacatgtaa ttcactcctt tattattttt aaaaaccatt tttaaataaa
     3661 aaatctttat ctatatttga tttatggtta ctactattag aatttaattc cttgtagtta
     3721 ttagccataa aattattaac atactttcca attacatcgc tttctaaatt aacaaaatct
     3781 ccaacattct ttgtcaagag aatagtgtta attttagtat gaggtataat agaaacctta
     3841 aatatttcat catctaaata tgatatagtt aaacttacac catcaatagc aacagaacct
     3901 ttgagtatca ttgaagataa aagtttttta tcaggtcgta tacttactaa aactgcattt
     3961 tcatcttttt caaatttgca gatagttcct ttaccatcaa catgacctgt tactatatga
     4021 ccaccaaatc ttgaagaaag actcatagct ctttcaagat ttaccttatc atttgcattt
     4081 aataaaccta aatttgtgga acgaattgtc tccatcataa catcggctgt aaattcattt
     4141 tttccgaggt ttgaaacagt tagacaaact ccatttacgg ctatactatc tccaagattt
     4201 gtgccatcta atattttatt tgatagtatg gttacttttc cactcttacc attagcagta
     4261 atttttctaa gtattccaac ttcttctact atgccagtaa acatcctaac ctcctatatg
     4321 aatgtattaa caaaaattta atttaaaaca tctgattcta taagaatatc gccatctatc
     4381 aaagttacat tattgattgt taactgtgtt gaatctgaca aatgatttat tccttctcca
     4441 gaaacgaatg taggtgcatc tttaccacct ataatcttgg gtgatagaaa taatttgact
     4501 ttatcaacta acttatttct aaagagactg tcattgagag tagcaccacc ttcaactaga
     4561 acagaatcaa tgtttaactc tcctaattta tacattacaa attccaaatc tactaagttg
     4621 ttttttgaag gagaaattat tgtttcaaca ttattttctt ttaacataga taattttata
     4681 ctttctttgc aacatgtgaa aactatagtt ttactggttt tatctttaac caaattacta
     4741 ttaagtggca actttaggtg tgtatctatt actattcttg taggatgaga cactttttct
     4801 tgattgagcc tgcaagttag taaaggatta tcgttcaaaa ctgtatttat gccaaccatt
     4861 atactttggt atttatttct gtatttatga gttagatacc ttgatgattc attagatatc
     4921 cattttgatt ctagtgattt tgtagcaatt ttaccatcta gacttactgc tgattttaca
     4981 atacaaagag gtcttttgtt ttttatatga taaaaaaatg cctcatttaa atctcttgct
     5041 tcatcttcta atattcccac agtaacatca atattgttat cttttaattt ttttactcca
     5101 ttaccacaaa caagtggatt tgggtctaat gtagatatta caactctctt aatcttattt
     5161 tgtataattt tatctacaca aggaggcgtt ttcccataat gagaacatgg ttctaagttt
     5221 acataaagag tagagtcttt tagactttgt ttagcactgt ttatagcatt tacttcagca
     5281 tgattagagc caaatttctc atgatatcct ttgcctatta tattactatc tttaacaatt
     5341 acacacccaa ctaatggatt tggatttaca aagccttctc catttttagc caattcaata
     5401 gcttttttca tataataaat atctttttct ttttgattca caatatgtac acctcctgtt
     5461 taattggata atttttaaac aaaaaaccct gagaattaac tcagggttta gaataatcta
     5521 caataaatgt ttaataaaag tagaagaaaa ttgctctaca aaaacactca ttaaatatct
     5581 tcttccatcc agactttact gtcggctttg gaatttaacc aaatcaacca agttttacct
     5641 tggctcgcgg gctataccgc cgattgggaa tttcacccta ccccgaagat taagtattaa
     5701 atttatattt agaatgtatc ataaattatt ttactttgca aactattttt ttaaaaaata
     5761 tatgttaata tataatatag aatgtctata aactttgaaa taccacgatt gtgaatataa
     5821 aaataaa
//
LOCUS       DAEPOZ010000223         5801 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.223, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000223 DAEPOZ010000000
VERSION     DAEPOZ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5801
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.223"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     repeat_region   18..179
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=20..48
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
     gene            complement(570..2303)
                     /locus_tag="KR440_003126"
     CDS             complement(570..2303)
                     /locus_tag="KR440_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBH0700136.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDINEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDVFETVSEDENRCMVLGRYTVRTKENAQMIL
                     EVNQRCTFEIIEDRGKLLVKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDSTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     gene            complement(2543..3388)
                     /locus_tag="KR440_003127"
     CDS             complement(2543..3388)
                     /locus_tag="KR440_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH0700137.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKEKNKKKF
                     DKNDY"
     gene            complement(3419..5653)
                     /locus_tag="KR440_003128"
     CDS             complement(3419..5653)
                     /locus_tag="KR440_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0700138.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQYIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESSRELKIRLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLILFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
BASE COUNT         1961 a          824 c          673 g         2343 t
ORIGIN      
        1 agtttgccat atgttcctgg ttttatatta actaagtggt atgtaaatta ctttagcact
       61 agtaaaggta gaattattaa agtgttttat attaactaag tggtatgtaa attcgaatgt
      121 gaatggcact tctatttctc caacttttgg ttttatatta actaagtggt atgtaaatag
      181 tataatgttg aaaagttaga gagtacaatc aagaaatttt acattaacta aaatgtatag
      241 aataaaacat gtatttatac ttaaattctg tacctatata aaaaagtgaa ctctgtcaac
      301 aaagcacttt tttatataga taaattatca ttttatttta agatagaaga tactaatgct
      361 aaatgtttat cattagtatc tgtatgtaca taaaagttta attttttata taaatttgat
      421 ctttagaaaa atgagcagta tcaataaata tattgtctaa attttttcta ggaactagtt
      481 gactagctat aagattagct tcaactcttt gattgttaga ctatgaaatt aaatctaaag
      541 gttcattctt ggtcgtataa atagctttat cattcgtatg tactataaca atttttgcca
      601 tctgcttttg atagataaag agctttatca gctttagaaa ataaatcttt atataattta
      661 gttgaatcat cagtgaaggc aataccaata cttaatgtta ttttatgatt gtcctttact
      721 tttattttac ttgcatcatt taaaatatca ttagtaattt ttattattaa atcctttgtt
      781 aatgatttta taaatattgt aaattcatct ccaccaattc gagcaactat gtctttatca
      841 tgtgtgttat gtaatagtat ttttgataca gcaactaaaa cttcatttcc ctctaaatgc
      901 ccaaaagtat catttacagt tttaaaatca tcaatatcaa taactaatag agcatcatta
      961 tttttggaat cagaactaca tagatactct tcaataagat tttgtgatac tctttgatta
     1021 tataattgtg ttagtgggtc acgttgagcc tgattaatta agctttctaa tttagattta
     1081 tcttcagtta tgtcttttaa aacgcctatt gttttgatag ggttatggtc actatcatat
     1141 ataactgaac attgaacacg aatccatctc cattcatttt tagttaatga atttacttgt
     1201 cttatctcaa ttgtttcacg aagatttcca tgaagtactt ctagtagttt tccaatatca
     1261 tcaaggtgaa taattttacc agattctaat agtttggagt aattttttgt ttcaaaattt
     1321 tctaattcaa tttttttgtc aatttctaca cgttgatatt ttataagggt atcgttttca
     1381 atatcatact caaacatgat atctgtaata ttttgcaaag caatacgata tcgctctctt
     1441 tctaatttta gttcagattc tatgtttttt agttgggtta tgtcagtaat aatactatta
     1501 atttttacta tgccatcact atttaacgat ttcattccag aatctaatac ccacattaat
     1561 gttccatctt tttttcggat tctatattca gatgaatatg taggtccttt tgcaaaacat
     1621 cgttcacaat cttctagtgc tttaggtaag tctggtggat atacagcacc cactgctgaa
     1681 ccaccactca tttccatgaa ctcatcatat gtgtatccta atattttggg caatccctca
     1741 ttgacataga aaaatgagta tgtactatca tcattgctac cttttaagcc accattgata
     1801 ttatttgtaa tcatttcaat aacttctgtt ttttccttta gtaatctttg caaataatca
     1861 taactttgag ttccaatttt agtgggaaag tattcatcaa gttgcatttc accataaggg
     1921 tttgatatgt gcatgtgctt tactaataat tttcctctat cctcaattat ctcaaatgta
     1981 caacgttgat ttacctctaa aatcatttga gcattttctt tagttcgaac agtatatctt
     2041 cctaatacca tacatctatt ctcatcttca cttacagttt caaatacatc attatttata
     2101 tcacaagaag gaatttcatc ttgacctgca taaaaataat tttttatttc attaaaagaa
     2161 gtgagaaatt cctgttctcc tggtccaatc catgtaacat tttcacaaag gtattttaat
     2221 acttcattga tatctcgatt tatataaaaa cttgttataa tatgttttgc taatttgggt
     2281 gcttttttta ggagtatgct catgatattc ctcctttctt tttgctatta tattttaatt
     2341 atattataca tcaatatatt taaattaaaa gctttgttaa actatgatat ctttgtcaaa
     2401 taagttaatt ttaacaaaaa agaactgaaa caatatatgc ttcagtactt aatgtcaata
     2461 tcttgtatag atacatattt ataaactaat gtacaagttt aaaaaaagaa agataatttt
     2521 aatttatttt aaaataacaa tgttaataat catttttatc aaattttttc ttgtttttct
     2581 ccttgttttt atacattttt ttatccaatt caactattgt caattttaaa tttcttttta
     2641 ctgtatcaac accataacta aatgaaagat acttatattt agagaatact tttaaaaaat
     2701 tttctcttaa ggcttctagt tgtgaataaa cataattttt gtcaacatca ctagagaata
     2761 taacaataaa ttcatctcct cctaatctta taatagaagt agaatttatt aattctttat
     2821 ttaaaagtga aacaaaatca cataaaatct tgtctcctac attatgacct tcatagtcat
     2881 ttatcatttt gaaattatct aagtcaatta gtacaatata atctcctata aatatgtcaa
     2941 atctatcatt taaatatttt ctattatatg ctccagttaa tgggtcagta attgagtagt
     3001 gaagatttct cttattttct tcatagagtt ttgttatatc attggccata gtaattatgt
     3061 aggttttttc atcaacatct actttgtatt ctaaaacatt ccaatagctt gtagtatctt
     3121 ttttaaactc taaaatccaa tttgaaattt tatcataagg ataatttttt tcccaatcta
     3181 attgaatatt gtgagaatcc gtaaaaaatt tagcaatatt atcagggagt ttcttagcta
     3241 tttctatatt agtcattcca agtggattat cttgcatata acttttaatt aaatttttta
     3301 aattatcatt tataaatata attctgtagt tagaatctct tataattaga aatcctggaa
     3361 aagttcttaa aaaaatttct ttaaacatat taatttctcc aaaataaaat actttgtact
     3421 agatttttgt taaatttttt attttattta catctacata cataccttgg gcaatcgacc
     3481 ttccatcaat tgcagaaagg ttacattgtg tatacataac tgtgacaatt tgaccatctc
     3541 gttttatgaa tctatataaa aatggttcaa ttttatttgt gtcaatcata tccaaacttt
     3601 ttttataaac aaagtctaaa tcatctggat gaacaattgc tcttaaattt ccgcccatct
     3661 caccaaaaaa ctgttctttt gtataaccta taatgtcaaa catttcttca ttcatataag
     3721 taataatcat atcaccaaat aaatcgtagc gaattatacc tacaggcaac attgaataaa
     3781 attgtgataa aatattactt tcatcaagat gtgtatttgt cattttaggt attgaatatg
     3841 attcaaattc atttatatag ccagcagttt taattaaatc caaatgtaat tttgctaaga
     3901 gtaatttatt ttctgaaatt atacaatttg cactaaagta gaaattgcta tatttttgat
     3961 gttcagaagc ttcatctgat aaaacggctt ctccactaat taataatgaa tctgattcag
     4021 tattatgtgg ggttatactt ttgtaaagaa tagaaaattt tttaccttta atatgttttt
     4081 caaaatgttc tattacttta ggaaaagatg taatttcagt tttgttgtaa aaatcaaacc
     4141 attgaatgtc ttcagtgaca ttagctctta gttttttcat atcttgcata ataaaaaatc
     4201 catcaaatat cttctctatt aaagattttc ctatatcaga atattttata aacattttct
     4261 ctgaattatt ctctttagat agtaattttt caaattcttt gcactccatg ggttttgcat
     4321 aataataacc ttgggcgata tcacaattta tacttttaat aaattctaca tgttgttctg
     4381 tttcaacacc ttcacatact gtaagcatcc caagagctct tgcaatgttt attatagagt
     4441 acaaaatggt tgtattttta aaatcattta aattatgaga taaaaatatc ctatcaattt
     4501 tgattatatc aaaaggtaga ttttgcaaca taccaaaaga tgaatatcca gaaccaaaat
     4561 cgtccattga tacagaaaaa ccataagaac gaagttcagc tagttgttga ataattgtat
     4621 ttgtattttc tattaatata ttttctgtta tttcaatgct aaaatttgat gtagagacgc
     4681 tgtacttttc agtaatacct tttagcatac ttggaaaatt agggttaaaa aaatgaagac
     4741 gagaaaagtt acaacaaatt ggaacaaaaa catcattgtt atcaatccat cttcttatat
     4801 ttttacagtt ctcttcaaat aaatgcaaat ctaattccgt tatcagacca aactgttcaa
     4861 ataaaggaat aaatttatct ggagtaagaa gtcctttaat agggtgtctc catcttgcaa
     4921 gtgcctcaca tccaactata gtattatttt ttatattata aagtggttgg taaaatactt
     4981 caatttgttt agtttgtaga acatgtgata tttctgataa gaactcctta tcattttcta
     5041 atttattgta aaaactatta tctttaaaat caaatagaat gtatctattt ttgccttcac
     5101 ttttagcctt ttttaaagca ctatatactt ggttgaaaag tatcaaagta gtcatgtttg
     5161 gttcaccatt ccagatagtg ctgccgatac taaatgtaac attggagtat atttcttttt
     5221 cattaggaag tcttattttt aattctcggg agctctctat aatagttgca cccatattat
     5281 ttaactcttt atttgataca tttggaagta caattaaaaa accttctcct tttaaataag
     5341 cgactattcc ctttgattct ggaataaagt gcaataggtt ttgagttaga gtaattaata
     5401 aattttctcc gactagatat ccatatatat cattaaataa tttaaagtta tcgatattta
     5461 ataacatgat tcctatatgt atatttttcg aaatacagtc atcaatatat tggtacataa
     5521 attctctgtt tggtatttta gaaaaaaaat ctatttcatt taaggttttt aatttaaaat
     5581 ttctactatc taattcctta gataatactt ctaccttttt atttaacatt tttatataat
     5641 tatgattttg catggtattc ctccataaga tactttaaat tttgatgaca actgtattta
     5701 tgctagaagt atttaaatgt taatttgtaa ataatttaaa tgctaaattt taaataagta
     5761 tttaaaatga atatacatta agttatttgc tccaatgcta c
//
LOCUS       DAEPOZ010000224         5786 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.224, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000224 DAEPOZ010000000
VERSION     DAEPOZ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5786
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.224"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..789)
                     /locus_tag="KR440_003129"
     CDS             complement(<1..789)
                     /locus_tag="KR440_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBH0700139.1"
                     /translation="MIESKKSEKLLLSIIYIIFLVYILMIIRIILFRDVPIYAIFKGT
                     FRNVNLIPFYTIYQFIVDSNLDFMKATVNIIENIGIFIPMGIFLPIVCKNLNKKTIII
                     VIILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGINIYINMNKLFPNDIKRFKAII
                     ATSLVLGVLGLGVISKNYHNLLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTF
                     ESFKKGIITIKAGSNNKVKRPENLIDSDGNIRIYLNENTKLVSYI"
     gene            complement(947..1264)
                     /locus_tag="KR440_003130"
     CDS             complement(947..1264)
                     /locus_tag="KR440_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700140.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
     gene            complement(1371..1874)
                     /locus_tag="KR440_003131"
     CDS             complement(1371..1874)
                     /locus_tag="KR440_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0700141.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVIHS"
     gene            complement(1926..3125)
                     /locus_tag="KR440_003132"
     CDS             complement(1926..3125)
                     /locus_tag="KR440_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HBH0700142.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVINVYEDGKNIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEENKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAISLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            complement(3385..3573)
                     /locus_tag="KR440_003133"
     CDS             complement(3385..3573)
                     /locus_tag="KR440_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700143.1"
                     /translation="MNYHTKEELMEVLRVASSRIINCEKVQKKFSEETSHHTRFKNII
                     EAMYISKSLIMDEISKRD"
     gene            3908..4876
                     /locus_tag="KR440_003134"
     CDS             3908..4876
                     /locus_tag="KR440_003134"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBH0700144.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTKL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPNTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLRECIALSKTL"
     gene            complement(4993..>5786)
                     /locus_tag="KR440_003135"
     CDS             complement(4993..>5786)
                     /locus_tag="KR440_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBH0700145.1"
                     /translation="FPKENLPNRKVMSDINISDIESLAYKTREFWDLNSTPIVNMVSL
                     LESKGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYI
                     ISSELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFR
                     AYNLEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNS
                     IVSSLEEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
BASE COUNT         1993 a          950 c          535 g         2308 t
ORIGIN      
        1 tatataagat actaattttg tattttcatt taaataaatt cttatatttc catcagaatc
       61 aattaaattc tctggtctct ttactttgtt attagaacct gctttaatag ttattattcc
      121 ttttttaaag ctctcaaatg tcccaactat atctactgta tctttattta tatctttaat
      181 tatttcccta ttttcttcaa ttaatatttt tgatattttt ctatcaggtc taaatttaaa
      241 agttagtaag ttatgataat ttttagaaat aactcctaat cctaaaactc ctaaaactaa
      301 actagtagct attatagcct taaatctttt aatatcatta ggaaataatt tattcatgtt
      361 tatgtaaata ttaattccaa ttattccacc taatgaattt aaaataacat catctatatc
      421 actacttcca agagcaaaaa tatattgtgt taattcaaat gctaggctaa caagtataat
      481 tactataatt atagtttttt tatttagatt tttacatact ataggtaaaa aaattcccat
      541 aggaataaat atacctatat tttctataat atttacagta gctttcataa agtcaagatt
      601 actatctact ataaattgat atattgtata aaatggaatt aaatttacat tcctaaacgt
      661 tcccttgaaa atagcgtaaa ttggtacatc tctaaataaa attattctta taatcattaa
      721 aatatatacc aaaaatatta tgtatataat tgacagtaac aatttttctg actttttact
      781 ttctatcact gataagtctc ctttcgctat taatatttta agtatatcaa taaatattta
      841 agacgatttt aaaatttcct taaaatttcc ttaattttca aacaaaaaat caaaaaagcc
      901 tacacttaaa tgtgtagact ttaataaatt ttcatacaaa tacattttat tgaataacga
      961 acaatcttaa tgtagaaaaa actataacta cagttaaaag aagtctagca tataaaaaat
     1021 ttttttcttt caacataact cctgcaactc caaatataaa aaatagagaa gcaataaatt
     1081 ttccaataag attattatta ggataataac taaaaagcat aaaacaaagt acaaatgtaa
     1141 gtactcctat tgcattaaac acaattttat tagatgctat ttttccttta ttgaaaaaga
     1201 aacagaatat tgacactaaa tctaaaaata aaaatacata aaacattcct tttaaaacgc
     1261 ccatttttat accccctaaa aatcctaatt ataatatcca gtttatttca tataatcgtc
     1321 aatataatca atactttata acatcagtat aacagtatta caaatttata ttacgaatgt
     1381 attacttcgt attcatacat attataatca cactcataca tatttaaaga ctcatatgtt
     1441 gcttttgcat ttatgttttc tttttctaca tataatctca ttccaactat attttcatct
     1501 ttatcacata tattttttat ataattaaat aaataattaa agataccttt tcttctatat
     1561 tccttatcta catatacact ctgaatccaa aggaaatttc catttctcca atcactccac
     1621 tcatatgtat acataatctg tgcaacaact ttatcaaata ctgtatatac atgatatttt
     1681 ccttttctct catctaataa caatgctttt acacctttag ttaaaacatc catatcaagt
     1741 tcttttcctt cagtttcttt agccaaatta taattaaatt ttgctattgt atccaaatca
     1801 tccatacgtg cttctcttat ctctattagt tcttcatttt catataactc tatgtagtcc
     1861 ttttgttgct ccatacttat tcctccatat ctattttatc tactaaggtt ttttatataa
     1921 tatatttata gagttttttc tatcttcata aatgcataat caagtgtagc ctctaaattt
     1981 ccattattct tacctcctcc ttgagctaat actttacttc caccaccctt gccatctata
     2041 agacttattg catcctttaa taaaactccc atattaatat cttttaaatt tttagaacat
     2101 gcaaaaacta aatttatttt atcattaaat ttaacagcta ataatgcaat actattttca
     2161 tattctgcaa ttttatttgc tatcttattt acatatttca aatcttcatc atcataaatt
     2221 tttttaatga ccgaaatatt gccaacctta tttgcttttt caatcatttc tttgacctga
     2281 tatgtagata ctacttcttc aagccttctt tttttaatta aagcctcttc cactttacta
     2341 tttagattta caataccatt tatagcatct tcctcactac agcttaaata tctacatatt
     2401 ttagtcaaat atatatccct cttcaatact tcatccactg cccttttacc tgctagaaac
     2461 tctattcttg tacttgactt atgtttttcc catttcttta tttttatcat tctcaattca
     2521 ccagttgatt ttggatgcac tccacaacat gcattcgtat ctaaatcacc tatttttatt
     2581 actcttatct cttcatcagt atttggcaaa tcacgtctta accaaatctt ttttagttct
     2641 tttttcgttg gaagtaatgt ctctacttgt atattttggc gaataatttc attggcaaac
     2701 aactctatct ctctaatttt gttctcttct aaattacctt ctatatctac tgtactatac
     2761 tcatttccta aatgaaaacc tgtcgtattt gcattaaaag ttttaaaaaa acatccagat
     2821 aatacatgtt gtccaaaatg ttgatgcatt ccatcttctc ttctatccca atcaatacta
     2881 cactttacct tatgtatctt tataggtttt ttatctaata catggtatat attttttcca
     2941 tcttcataca catttattac atcatcattc tctatttttc ccaggtctcc aaactgacct
     3001 cctccaccag gaaaaaaagc tgtcttatct aataaaacat gatacttatt ttctacttct
     3061 tttatttcaa ttatttctgc tgtaaaatct cttatatact gatttgtata atataacttt
     3121 tccattataa ttctatctcc attttcttaa ttatagacta ctttactttt taaatacaaa
     3181 tttcaagcta agcttaattt aattgtatta aaatagaact catcctatta tttactaaca
     3241 tcttctacta tattttaaca tatagatatt agtgttaaca aatttataat tttatattat
     3301 ttcaatattt tataacaagt atgcttactt attttcttat ataataaaat gtatattaaa
     3361 acatattaaa ttgcaaaata agcttcaatc tcttttacta atttcatcca ttattaatga
     3421 ttttgaaata tacattgctt ctataatatt cttaaaacgt gtatgatgag atgtctcctc
     3481 agaaaatttc ttctgtactt tttcacaatt aataatcctt gaagatgcta ctctcagtac
     3541 ttccatcaac tcttcttttg tatgataatt cataacacat ttactccttt gttttttaat
     3601 ttagagaaac agtatacctc aatttaatat cattatcaac tgatatcatt attatttata
     3661 tatatctttt aacacttaaa tattctaact atcagtgaca ttacatattt taataattat
     3721 taatatacat ataatacttt aacattcatt aatagagagt atattaacat tgctttttta
     3781 aaaattttag tataaaatca tattaaataa attttctgaa ataataaaat attaaaatat
     3841 ccaaaaacag cttttgataa catctataat tttcaatgta tcgttatttt agggggagtg
     3901 atttaaaatg gcaataaagc caagaaaaat atctattgta ggttcagggc atgttggttc
     3961 tcattgtgga tttagtttaa taactcaagg agtttgtgat gaactcttta tgattgatat
     4021 agatgaatct aaatcaaaag ctcaagcttt agatttagct gatgcagttt catatctacc
     4081 acataaagta catatagaaa aaggaacttt ttctgactgt aaagattctg acatagtagt
     4141 gataagtgtt gcagactctt cagaagggcc tttacgtaga caaaacacaa ctagattaga
     4201 tttactaaga ccaactattg gaatgataaa atctatagta aaacccatag ttgattctgg
     4261 ctttgatggt atatttgtag tcatatctaa tcctgttgat gtagttacaa attatatttg
     4321 ggaaaaatct ggctttccta aaaataaagt cattggaact ggaacagctt tagattcaac
     4381 aaaacttaga aggattttat ctgaagaaac tggaatcgct caacaatcaa ttcaagctta
     4441 ttctatgggt gaacatggag attctcaaat ggttccttgg tctcatgttt caattggtgg
     4501 taaaccaatc ttggacatga taaaagacaa cccaaatact tatagcaatt tagacttacc
     4561 atcaatagtt gaaaaaaata aaaaaacagg gataagtata ataaatggta aagattgtac
     4621 agaatttggt ataggtactg ctttagtaga aattgtaaaa gctatactac acaatgagaa
     4681 aaaagttctt ccagtttcta cattactaga aggtcaatat aacgaaagaa atgtatttgc
     4741 aggtgttcca tgtgttatag gaaaagatgg tattgaagaa attattgaaa tcaatatgac
     4801 tgaatatgag caaaacgaat ttaataaatc ttgctctgtt ttaagagaat gtattgcact
     4861 ttcaaaaaca ctttagattc aatattcttt cttacactaa aagcaggtaa atgacttatt
     4921 gaaaagactt ttaactaagt taatgtttaa atcatttata cctgctttta attttaatac
     4981 taaaatataa acctattttt taccatcaaa ttttataact ttactttttt tatttacctc
     5041 ttgagataac aatccttttt tcaatcccat caataattct aagtcttctg gataaagatt
     5101 tattccaaat tcctctaaac ttgatactat actatttaca ctcattattt tattttctat
     5161 caataattca actgctcttt ttaaatacgt tgggcttgaa cctttcattt tgtctagtgg
     5221 ttcttctatc aaccagcctt ttttatccat ttcattcata agataattgt attttttgta
     5281 atttatcttt tctagattat atgctctaaa aatcatagca gatataggta caatccattt
     5341 tttctttagt tctacataat aatctaaatc ttctggatga gtcaaatctt ttaaaaaact
     5401 ctcttttggc aataaaaatg cacaggcata ctcatcttct gaaaattgct tacttggtat
     5461 tcttaattca cttgatatta tatatccaag ctcacatgcc aaagtataat ttctaattga
     5521 tgctgacttt ttgtcattac ccagagatat tagatattta gattctttac taattctctg
     5581 tttttgagta aagatagttg ccccttttct atctacattc atacctgata ttataatccc
     5641 tttagattct aacaaactta ccatatttac tattggagta ctatttaaat cccaaaactc
     5701 tctagtctta tatgcaagag attctatatc acttatatta atatctgaca ttacttttct
     5761 atttggcaag ttttcttttg gaaact
//
LOCUS       DAEPOZ010000225         5763 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.225, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000225 DAEPOZ010000000
VERSION     DAEPOZ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5763
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.225"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(970..2376)
                     /locus_tag="KR440_003136"
     CDS             complement(970..2376)
                     /locus_tag="KR440_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0700146.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLILKDGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTIKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGVKIRELVNDLNLVSKLEYNMQALNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKSQNGLSEAYAKGQNHGLGILIVAGIINAHRGKLDI
                     MSIDSQGLKTILKLPLIK"
     gene            complement(2421..3128)
                     /locus_tag="KR440_003137"
     CDS             complement(2421..3128)
                     /locus_tag="KR440_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0700147.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            3290..4213
                     /locus_tag="KR440_003138"
     CDS             3290..4213
                     /locus_tag="KR440_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0700148.1"
                     /translation="MKHMEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGA
                     GKSTTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENM
                     TIMQRLLDLPKKNVNEALKIVRLENQKNKKVNQYSLGMKQRLGIAMAIMKFPKLLILD
                     EPTNGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQD
                     SIKELHNRGQSQIAIKAKNVVSAQKLLNTKGYMSLIQNGYLTIENLKDSDVAKINGML
                     VHSNIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            4213..4974
                     /locus_tag="KR440_003139"
     CDS             4213..4974
                     /locus_tag="KR440_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0700149.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFVAVEIMWCIVNSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPILMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            4986..5723
                     /locus_tag="KR440_003140"
     CDS             4986..5723
                     /locus_tag="KR440_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0700150.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
BASE COUNT         1985 a          908 c          705 g         2165 t
ORIGIN      
        1 taaatttctt acttgctaca aattattact actaaacttc ctactcccta aatttcttac
       61 ttgctacaaa ttattactaa acttcctact tcctaaattt cttacttgct acaaattatt
      121 actaaacttc ccactttcta aatttctcgc ttactaccac ttatcactaa acttcctact
      181 ttctaaattt cttgcttact accacttatc actaaacttt ttacttctta aatttcttac
      241 ttcttaagaa taatgttaat tttaatttta agattatagt ttttgccttg tttaattgaa
      301 acattctata taattatatc cttctatttt agagtttttt tatttatata ttacaaaatt
      361 taaatttttt cattaattat attataaatt tcccatatat ttcaacataa atacatatat
      421 atatatgtta tttctaaaaa tactacacaa aaactctact ttagtagaac tcaatggtgc
      481 aaaatacttc aatttaaaac atttatcgta ctttaaataa ataattataa taaaatatac
      541 ttacaatagt tacatagaag ctattataac tacacagaac aacgtataat taaaactttt
      601 ttaggatgtc attatatttt ttcttaaaat taaaaaacac tataccataa ttctatttta
      661 gtagaatttt tttgtcaata ataaaattaa atattaacta aaaaataaaa aaaatggaat
      721 tatctaaaga atttaataac cttacaataa ttccaaaata ttatttctat gaaaattttg
      781 ataccatgag taactcagcc ataagcgcat aaagcacaag ttactcttta ctttaataaa
      841 acttgacaag aaaagaattt atccttattc tactctaata gagttatttt gtcaataaag
      901 aataaaatta ttttcaaaat attttcattc ttcattcttc attcttatct gttattattt
      961 tataaaatat cacttaatta gaggtaattt tagtattgtt tttagtccct gactatcaat
     1021 actcataata tctaatttac ctctatgtgc attaataatt ccagctacta tcaaaatacc
     1081 tagaccatga ttttgacctt tggcatatgc ctcacttagt ccattttggc tctttaataa
     1141 actattaatt ctttcttgac tcataccttt tccatcatct tcaacaatta tatagcaaaa
     1201 atctaaatca gacttagagg taacccttat attacatcct tgttggttat gagttatact
     1261 attttgtatt aaattactaa tagccctttt taataatttt ttatctgcat ctataataat
     1321 atcttcattt agtatatcaa gttcaacata aaagtcatta tccaaattat tatttataaa
     1381 ctcagaaata agctctctca aaaacgcaga aactctaatc ttatcacaat tcaatgcttg
     1441 catattatat tcaagtttag atactaaatt taggtcattt actaattctc tgattttaac
     1501 cccttgattg catataatag acacttgctc ttgttctcta tttgaaaggc tactgctttc
     1561 ttcaagttct ccagcatatc caattatcat tgaaagtgga gttcttatat catgagatat
     1621 tcctgcaatc cagttagacc tagcttcctc tttcttccta agtttttcat ctttcatttg
     1681 caactcttta gatacagtat taatagattt tgaaatatca cttagtattc ctttttcttg
     1741 taaaagtact ggttctcctt ttgacataag ctttatgcca gtaataattg gttttataga
     1801 tttcaccatt tttaaaccta tgaatagagc tattaaaaaa gtaactattg cattaaccag
     1861 aaacatacct attattaaca taggaaattg ttctatttcc ttaatcgtat aagtaaggtt
     1921 aaatttagtg tatttgtcct ttggataacc tgttactaat attccatttg gatgttccca
     1981 tacatacaca ggatagtcct ttaaaaagaa ccttgaaaac tttgctacat ctgtcaaagt
     2041 ataatgactt ggaatttctt ttggtaaatc aaatccccat attctatctc cattgttatc
     2101 aattaacatt gcccaaactt tatttttatg tagtacttcc ttactagaat catctaaaat
     2161 aaactttcca tcttttaaaa tcaaattatc ttcaatcata atcaagtttg attttatttc
     2221 tctgctatca cttgtatcta aattatcaga gtatttagat acaaatagca tatatgaaaa
     2281 cagaagaaaa cctgctatat ttaatacaat aaaaagtaat gaaatcaaga tacttgaacc
     2341 tataaatcgc ctcaatatgt actttacact atccaaaatt aaatcactcc tcttgatttc
     2401 ttttattcta atatctaaaa ttaattaatc atcaacttat atccaagacc tcttactgta
     2461 ataagatgtt taggtgtaga tggagttttt tctatcttct ctctaaccct tctaacatga
     2521 accataaggg tattttcata tccatacata tcatctcccc aaatggcttg acatagtgaa
     2581 tcattagtaa caattctccc tttattttca tacaacttta aaagcaacga atgctcttta
     2641 gctgttaaag aaatctcttg cccatctttc tcaacacatg cactatttaa attcacaaca
     2701 acagaatctc ccagtttaaa tactggcaac tcctcttctt ctataggcac atatacacga
     2761 tttaatatac ccacaagcct taaagtaagt tcctttggta aaaatggctt tactatataa
     2821 tcgtcagccc ctaatcccaa accaatcaat ctatcttcat cttcacctct tgcagagaga
     2881 aaaataactg gtatcttaga tgtctctcta atttttctca taatagagaa accatctcca
     2941 tctggcaata caacatctaa aattgcacaa tctggcttta catttctaaa taccctaaca
     3001 gcttcttcat aacttgatgc taaaaatact ctactaaatc cctcttttct taggatttca
     3061 tctatcattt ttaataattc cttgtggtca tcaaccacaa gtattttttt atcttttatt
     3121 gactccataa tcatcaccct cctttagtat accatatacc cattttcaca actttgccta
     3181 aatagacttt taaggttatt gtaaggtaaa cttcagctaa ctgaaatgta accaagttat
     3241 tatactgata taagttaaaa gaatcaataa gcacagatta aaaaagggga tgaaacatat
     3301 ggaaatgata attagaacac aaagactttc aaaggaatac aataaaatat ttagagtaaa
     3361 ggatatagac ctaagagttc cacaaggagc tgtatatggt tttttgggtc ctaatggagc
     3421 tggaaaatct accactctta aaatgcttct tggacttgct aaacccacac aaggaaatat
     3481 taatatttta ggtaaagagt taaatgaaaa aaatagaata tcaattttaa aagatatagg
     3541 ttctttgata gaatctccat catactatgg tcatcttaca gggcttgaaa acatgaccat
     3601 aatgcaaaga ctcttagatt tacctaaaaa aaatgtaaat gaagcattaa aaattgtaag
     3661 gttagaaaat caaaaaaata aaaaagttaa tcaatattct ttaggtatga agcaacgtct
     3721 tggcattgct atggcaataa tgaagtttcc caaattatta atacttgatg aacctacaaa
     3781 tggactcgac ccagcaggga ttgaggaaat tagagaatta ataaaatctc taccagaaaa
     3841 atacggcatg actgttctaa tctctagcca tcttttaagt gaaattgacc aaattgccac
     3901 atcaatagga ataataaatc atggcgaact ggtttttcaa gatagcataa aagaacttca
     3961 taatagaggt caaagtcaga ttgctatcaa agctaagaat gttgtatctg cacaaaagct
     4021 attaaatact aaaggatata tgtcacttat acaaaatggg tacttgacta ttgaaaattt
     4081 aaaagactca gatgtggcta aaataaatgg tatgcttgtc cattcaaata tagatgtatt
     4141 tagaatagaa gaacataaaa aaagcttgga agatatattc ctagaactta ctggtaaggc
     4201 ggtgacttta taatgaaata ccttggtcta gaattttata aacttaaaag aaagaagatt
     4261 attctgatga cctttttatt tgtagctgtt gaaataatgt ggtgtatcgt aaattcaaat
     4321 agggctatta cacgtaatcc agatatgtta ggtggatttg aatattccta tatgcttatg
     4381 agttttgcaa gtttaaatgg tctattcttt cctatcttaa tatcaataat tacatcacgc
     4441 ataagcgata tagaacataa aggagataca tggaagcttt taaaatcttc tgtgacatct
     4501 ttaaatagta tttatttatc caaattttta tgttctgcta ttttggtatc tatacctata
     4561 ttgatgcaag tgttatctat tgtgttgttt ggacatttta gaggggttat tgagtcaatt
     4621 tctgtatctt tacttttaaa atacacatta ggaacaattc ttgtaagtat tgctatcatt
     4681 gcactacacc tatggattgc aactgtaatt ccaaatcaaa tgattgcaat tacatttggc
     4741 atgattggta gttttatagg tttgacatca ggtttgtttt atcaaggtat tcgtagattg
     4801 tttatatggt cttattacct agagttatca cctttaagtt atgcttatga taatgtttta
     4861 ggttcatctg tctatagaat tgatatgaat ttttctatta cgattttagt tttcttaatt
     4921 ggaatgctaa tttattatat tggaagaaat catttattaa aaaaagaagt ataggaggga
     4981 tttaaatgtt aaggaagtca gttttgggag aaattctaaa gttaaaaaat tcttttatat
     5041 ggtatgtatt attagctttg ccacttataa gtgtgttgat tggttctggt aatttttatc
     5101 taaatcaagg catactcaaa aaagaatggt acagcttatg gactcaagtt agtctttttt
     5161 atggggaatt tttctttcct atcttaatcg caatcttttg tgcatatatt tgtaggcttg
     5221 aacatatgaa tcataattgg aataatgtaa tgacactgcc agttaattta aaaaatatct
     5281 ttttatctaa acttacagta gtttcaatac ttacagctat tacacaagtt ttctttgtta
     5341 tcttttatat agttgcagga aatatgtttg gtttttctgg tagtgtacct aaagaattga
     5401 ctggttggat tgctaaaggt tggattattt ctatttcaat agcttctata caattataca
     5461 tgtctataaa gattagaagt tttgcaactc ctattggtat tagtctttgc ctttctctat
     5521 ttggtatggg attaatagta gcaaaacctg ctgttggtat atttttccct tattctatgt
     5581 taggtagtgc tatgggggtt ataaagcaat ctgctttaaa tgttaatgac actatagtta
     5641 caatatgtgt tgcatttatt actactatag tattttctga aattgccaat agaaatttta
     5701 gaaagaatga tgttatttct taatggtaaa tgctagatat tttttggttt gaaatttata
     5761 ttc
//
LOCUS       DAEPOZ010000226         5703 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.226, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000226 DAEPOZ010000000
VERSION     DAEPOZ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5703
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.226"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            14..610
                     /locus_tag="KR440_003141"
     CDS             14..610
                     /locus_tag="KR440_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBH0700151.1"
                     /translation="MDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQFLALKAVLNMD
                     GVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITIDAYGRIINVINT
                     HLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMAISLNKSYLPTF
                     EKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            849..2108
                     /locus_tag="KR440_003142"
     CDS             849..2108
                     /locus_tag="KR440_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBH0700152.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLTYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            2367..3362
                     /locus_tag="KR440_003143"
     CDS             2367..3362
                     /locus_tag="KR440_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HBH0700153.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRRNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNS
                     EEKMLQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            3608..4675
                     /locus_tag="KR440_003144"
     CDS             3608..4675
                     /locus_tag="KR440_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBH0700154.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHTGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSEDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYSKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            5212..>5703
                     /locus_tag="KR440_003145"
     CDS             5212..>5703
                     /locus_tag="KR440_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBH0700155.1"
                     /translation="MNSNTFERLQLNEVKDLIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNAGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKI"
BASE COUNT         2394 a          543 c          951 g         1815 t
ORIGIN      
        1 tattcataaa ggtatggatt caaataatag attaactcta actaaaatgg ggttatattt
       61 aaaacaattg gattgtgatg taatttgttt gcaagaagta ttatatcctc aatttttagc
      121 tttaaaagct gttttaaata tggatggtgt ctttgctaca aatgtaaaaa aagtaaatat
      181 gatatatggt atttgtacat ttacaacatc taaaatgtta aacagtaatc attttttctt
      241 aactagcaaa aaagaacaac ggggagctct ttgtataact atagatgcat atggaagaat
      301 tataaatgta ataaatactc acttgggact tgataggcag gagagagcaa aacaattaga
      361 cgaaataata gactatagga acagattagt ggggatagtt attttatgtg gtgattttaa
      421 tgagaaaaat gtctttttaa gtatgtttaa tgatatggct atttcactaa acaagtctta
      481 cttaccaaca tttgaaaaat caaactctag aatagattac atttttgtta ataaaaatac
      541 tgaattaaaa ggatacacag tagaaaaaat ttacctgtca gaccattatc ctgtaatagg
      601 ttacatttaa atttcaatat aaatgaaatt agttgttata aaataaacaa ttttttgtta
      661 agaaatagta aaagaaatat aataaaaaat atcaaaaata aaactaatta taagacataa
      721 atttacaata aagttacatt tgattaaatt ccttgtttaa caaaagctat tcaaatataa
      781 tctaaggtag ttttgaaatg ggaactctat tatgcaaatt taagctagtg tcaaaatgag
      841 gtgaagttat gggcagaaga acaacaagag tttcaagatt aggtaaagaa aaaaaacgaa
      901 gtgttttaat tgtattggca gctttagttc taataatggg tggatttact tattttttta
      961 acagtaaatt tctgtacaat gggaaaatcg ctaaaaatgt atatatagaa ggagtaaatg
     1021 tatctgatat gactaaagca gaggcattaa aagctataac agataagtat actccagaag
     1081 acttaaatct tacttatgat gggaaaaaat atacaatatc accaaaagat attgatttaa
     1141 aatatgatac agaagatgtt gtaaaagatg cttatgaaag tacaaaaaaa gggtcctatt
     1201 ttcaaaactt aaaaaaatat attgatataa gagtgaataa ggcaaatatg aagataaaag
     1261 ctgaatatga tgaggcaaaa cttagttcca aagtgtcttc aatagcagat agtataaatg
     1321 ttaagatgaa aaatgcttct attagtgttg gaagtggtgg actaagttat acagactctg
     1381 ttgtaggtag agaatttgat ttggcagcaa ataaagagtc tatatataat atgataaaaa
     1441 ataaagagca taaaacttta gaacttaaag taaatcttca aaaaccagat ataacaactg
     1501 aacaagtaaa aactgtaaat tctgttatag gtcaatattc aactacttat tcacaagctg
     1561 tagagggtag gtcatataat gtaggattat cagcaagaaa aactagtgat gtattattaa
     1621 tgccaggtga agaattttca tacaataaac ttacaggacc tagtaataaa gcaaatggtt
     1681 acaaagatgc acctgttata gtttatggta agctagaaca atcagcagga ggaggtgttt
     1741 gtcaaacatc ttcaacagtt tataatgcgg ctcttctttc gggtatggag ataactcaag
     1801 ttacaaatca ttcatctgca tctacttatg tgcctaaagg aagagatgct actgtgagcg
     1861 atggaggatt aaacttaaag tttaaaaatc catataagca tcctgtatat attaaaaatt
     1921 atgcaggtgg aggaagtgta tcatcagtaa tctatggtaa ttctggtgac aaaccaaata
     1981 tatctataga ggtaaaacaa actggtcaga ataaatattc tacatatcga atatttaaag
     2041 attcaagtgg aaaggtcata aaaaaagaac atatatcaaa tagttcttat aaagaattaa
     2101 aaaaatagat aattttttag agaaatattt gtaatataaa agggagtgtc tcaaaatgaa
     2161 ctttttagtt tatgagacac tctttttatg tgaaatcaaa tatattttca tcatttcaac
     2221 aatacaaaag aagcgtatct ctattttgag acagcctttt ttgtaaaaat taacactatt
     2281 agttcttgtt ttaatattac aatcattaat atatagaaaa tattcctata tattggtata
     2341 attgtaaata gatttagtta tataaaatgt atgaggaggt gacatggttg aattttatag
     2401 gcaatagata tgaggtagta aattctgatg acgttttaga aattaacaaa atctataaag
     2461 ctagagatgt tttttacagg agaaatgtgt taataaaagt tataaagcac aataattata
     2521 tttgtgaaga ttttgtttca aatttaattg atgaaagtac tgctttagat gagataaact
     2581 ctccttatat actaaaaata atagatgttg ggattcactg tacagaagaa acaacattat
     2641 attatgttgt aagtgaagac tttaatggta tcggactaga tgaattaatt ctaggtaatt
     2701 atcttcattt agaagcaatt gtaaacataa tgatacaagt tttaaaagct ttagaaatga
     2761 tacataggaa tcattcatat catggtagtt taaaatcgag tagtataata gtagatacag
     2821 aatacaatat aaaaatttgt gattttggaa ttacaaaagc aaataatggg gttaatacaa
     2881 gaagctatgg aaacaggaga tatttatgcc cacatcaact atgtataaat tatactgata
     2941 aagaaagtga tttttttgca acaggtctta ttctatttga atctatattt aaaaaattgc
     3001 cttttggaga atcaaattca gaagaaaaaa tgctacaatc tattgataag ggattagatt
     3061 ggaatagtat aaaagcaatt aatggaaata cagaacttat aaatgttata aaaaggcttc
     3121 ttggaagaaa taataagtat actagagcca atgatattat tattgattta agtaaagtaa
     3181 tgtatgaaaa agcatatata gaagttgaag atgagaaaga agaagaacag aaacaagaca
     3241 aagttataca agttaaaaaa gaatataaaa gtaaaatgag aaaaaaatta cataaaaaac
     3301 ttgttgttgc cggtctagct atagtcatga tttcaatgat tattattagt tccataatat
     3361 aaaaattgta tattaagtat tttaaaatca atgtctataa atgatataca tagaaatttt
     3421 agtgtagtct taaattatga aaggaagtgg ttattatgtt aacaaaaaat aatgaggtat
     3481 taaacgtcta ggagtatttt aacaaatagc cacctgcttt tacagtttag aataaaaaag
     3541 ctgtggctaa aggtcacagc tttttgctac tttttaaagg caggagaata ttaaaaagga
     3601 gcaaattatg agcaatataa aaataatagc aagtaataaa tgttggattg aagatatagc
     3661 aaggaaacaa ctagaagata tttcaaagtt aaatggaatt ttaagagtag taggattacc
     3721 tgacttacat acaggtaaaa tacctgtagg tttagcagtt gagactaaaa atattattta
     3781 tccacatata attggaaatg atataggatg cggaatgact ttatttaaaa ctggggttct
     3841 aaaaaagaaa tttaaaaaag acaaatggat taagtcactt agtaagatta aagatttgtc
     3901 cgatatagaa ataaaaaaca catataaaga agaatgtcca attttaaatt taggaactat
     3961 agggagtgga aatcattttg tagaaattca atgtataagt gaaatttata ataaagaaca
     4021 atttgaacaa ttaaaatttt catctgatga tattatgatg ttagttcact gtgggtcaag
     4081 aaattatgga gaagatattt taaaaaaatt ttatgataaa gatggacttg aagttgaaag
     4141 tgagaaagca atagattata ttaaaaaaca tgataatgct ttaatgtggg cagagcgtaa
     4201 tcgtgaagct atcagtaaaa aaattatgca gtcaataggt acatctgaag atgttgaaac
     4261 aatattctct ataaatcata actttataga gaaaagggaa gataaattta tacatagaaa
     4321 aggtgctgtt tcaagtgaaa gaggagctgt gattatacca ggttcaagag gcagtctatc
     4381 atatatagtt atgcctactg aaaatacaga gatatcttta tattctctat cacatggagc
     4441 aggtagaaaa tggtctcgtt cagtttgtaa atcgcgcctt aaaagtaagt atagtaaaga
     4501 tactataaaa caaactaaat ttaaaagtca gattatatgt aatgatgtaa atttattatt
     4561 tcaagaagct ccagaggcat ataagaatat agagcagatt attgaaagtt tgatagaata
     4621 tgaacttatt caagtagtgg caactatgaa gccattgatt acttataaag gataattttt
     4681 aaagttaaaa aagattaaaa ttatattata aatagctaat tttatggaac atttaaaagt
     4741 gttttattat tattagctat ttttttattt tatagagacg ataatttata gagctaaatt
     4801 ctttgatata taatgattac aaagttagat tgtattggaa actacacata catgaattga
     4861 tgacaatata agtaattcca aaatataaat gaaagtataa gcttattttc actataaaaa
     4921 ttttcactgt acaaaaacat taataacatt atttttattg acataaatat ataatttatg
     4981 gaataatatt agaagcactg gtataagtgt ttaaataaga atagaaaagg agaagtgttt
     5041 tatgaaaaaa aatactaaca ttaatgagtc cggagttaaa cttcttaata aataaggagt
     5101 ttgatatata ataactcctt atttattaag ttgttcgcaa aataactatt atcaaagtaa
     5161 atgatagggt gatttaaaag acgatgactt ataaataaag gagaaaaaat aatgaatagt
     5221 aatacatttg aaagattgca actaaatgaa gttaaagact taataaaaat acattgtgta
     5281 agttcgctag gaaaaaattt aatagataaa ttaactccta gtgggaatat tggagtagta
     5341 agaaggaaat tagcagaaaa taaagaagct agaaagataa ttgaaaatag taatcacata
     5401 ccacttgaag gattgtttaa tgcaggttct acaatagata aaattgaaaa aggaatgata
     5461 atagaacctg ttgaacttgt aaatattgaa gattttttaa gaggttgtag gaagatgaaa
     5521 gcttttatgt tagaaaaaga attttattca cctacattaa gttcttatgc tttaaatata
     5581 acagaatgta aaagtataga agatgagata aattactgta taaaatctaa taaagttgat
     5641 tctaatgcta gtaaagaatt aaaaaagata agacgaaata tagaaattac agaaggaaag
     5701 ata
//
LOCUS       DAEPOZ010000227         5662 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.227, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000227 DAEPOZ010000000
VERSION     DAEPOZ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.227"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..120
                     /locus_tag="KR440_003146"
     CDS             <1..120
                     /locus_tag="KR440_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH0700156.1"
                     /translation="DLSIREWICPSCNETHDRDINASINILKEGLRLITTQNK"
     gene            239..757
                     /locus_tag="KR440_003147"
                     /pseudo
     CDS             239..757
                     /locus_tag="KR440_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
     gene            784..2145
                     /gene="rlmD"
                     /locus_tag="KR440_003148"
     CDS             784..2145
                     /gene="rlmD"
                     /locus_tag="KR440_003148"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBH0700157.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMNEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVKL
                     IRE"
     gene            2668..3702
                     /locus_tag="KR440_003149"
     CDS             2668..3702
                     /locus_tag="KR440_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HBH0700158.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPYEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKNKKRKKI"
     gene            3789..3992
                     /locus_tag="KR440_003150"
     CDS             3789..3992
                     /locus_tag="KR440_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HBH0700159.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            4163..5323
                     /locus_tag="KR440_003151"
     CDS             4163..5323
                     /locus_tag="KR440_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HBH0700160.1"
                     /translation="MKLSKTLLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVIIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
BASE COUNT         2246 a          560 c         1110 g         1746 t
ORIGIN      
        1 gacttaagta taagagaatg gatttgtcct agttgtaatg aaactcacga tagagatata
       61 aatgcaagta taaatatatt aaaagaagga ttaagactaa taacaactca aaataaataa
      121 cttatgtaag aaccgtagga actacgggga tagcctgtgg agaattagta agacatattt
      181 tagtatgcaa aattctgtga agcaggaacc ctgtgacttt agtcatggga ggttcaggat
      241 gaataaacct gatggatata tatcagcaac tacagataaa tatgacccaa cagtattaga
      301 tttaattgat ttatcatact tagcttttga accatttcct gttggaaggt tagataaaga
      361 cacagaggga ttattagttt taactaatga tggaaagctt tctcatagag ttttatctcc
      421 taagaagcat gtgcctaaga cttattatgc aaaaattgat ggagtagtta ctgaagaaga
      481 tgtagaagct tttttagaag gtgtagtgct tgatgacgga tataaaacta tgccatcaca
      541 gttaaatata ttaaaaagtg atgatgaatc agaaatagag cttacaatac atgaaggtaa
      601 gtttcaccaa gtaaaaagaa tgtttgaaag cgtaggcaaa aaagtagtat acttaaaaag
      661 attgtctatg ggcaatttaa aattggatga aagtttagag ttaggtgagt atagagagtt
      721 aacagatgaa gaagtaaaat tgattgaaga aagataataa agatactata gtaaattgga
      781 ggaatgtctg tgaagaaaaa agatgttata gaatttgaag tagataaaat ggaatttggt
      841 ggtacatctt taagtcaagt tggagataga gttatccata tgaagggtgg aataagtggt
      901 caaaaggtga gagctggagt taaaaaagtt agaagtaaga aagctgaagt taagatgata
      961 gaattattag agaactcacc acttgaaact gaagaaactt gcaagcactt tagagagtgt
     1021 ggaggatgta cactattgtc tgtgccatat gaaaaacaat tagaaataaa agaaaaacaa
     1081 gttatggatt tatttttaaa gcaagattta tttggatttc agtttttagg gatagaagga
     1141 agtccagaaa acaaatatta tagaaataag atggaatata cttttggaga tgaagtaaaa
     1201 aatggacctt tgacattagg tcttcataaa aaaggaaagc atatagatat acaaactgtt
     1261 gaggagtgta tgttggttga tgaagacttc tcaaagatat tagtttctag tgtagagttt
     1321 ttcaatgaaa aaaagcttcc ttattataga acaatgaatc acaaaggata tttgagacat
     1381 cttgtagtta gaaaagggat tcatactaat gagattatgg ttaatatagt aacttcttct
     1441 caagaagatt ttgatatgaa tgagtttaaa gatatgcttt tagggattga tttaaaggct
     1501 gagttagtag gtgtacttca tactattaat gatggtttag cagatgcagt ccaatgtgat
     1561 gagttaagag tattgtatgg aagagattat atacaagaag aaattttagg tcttaagttt
     1621 aagatttcac cattttcgtt tttccaaacc aatactaagg gagctgaggt tctttatagt
     1681 attgctaggg attttattgg agattacaat gataaggttg tatttgactt gtatagtggt
     1741 actgggtcta ttggacaggt tatggctggt gctgctaaga aagtgtatgg tattgagata
     1801 gttgaggaag ctgttgttgc tgctaatgag aatgctaagt tgaatggact tactaattgt
     1861 gagtttattg ctggggatgt ggctaaggtt gttaaggatt taaaggacaa gcctgatttg
     1921 attattgttg accctcctag accaggcatt cataaggatg ctattaggga tatttgtgga
     1981 tttggtgcta aggagattgt ttatatttct tgtaatccta agtctttggt tgttgatttg
     2041 gttgatttta aggggtatgg ttatgagatt aagatggtta agtgtatgga tatgttccca
     2101 aatacgccac attgtgagac tgtagttaaa cttattagag aataacaggg tttcagactt
     2161 ttatattgtg tcttaaaagt agatttgcca cctatttgcc accataatta tttttgggtg
     2221 gcaaatttat tctacaatga tttcaaaaat atttaccatc tggtttctca tgttattagt
     2281 tacatgtaaa tatgtatcca tagttgttga agttttagca tgtccgagta tatgctggat
     2341 atcttttata ttagtaccat tttctaataa catagtatca gggtttttaa aataaagtgt
     2401 gtattcttct agagtagtga taattaaaat atcaaatatc actatacaaa ggaaagtgca
     2461 ccctttttat gtttagcaaa aacgaaatat taaaaaaact tattgaagaa tatgatgtca
     2521 aaacaactac agatattcaa gatatgctaa aagatttatt tacaaataca attcatcaaa
     2581 tatttccatt gtcgaatttt aacggtttaa acataatata ttgactgata taataaaatc
     2641 atagaatatt ttagggggaa atagattatg tttaagaaaa agttaatagc tgttgcaatg
     2701 agtataacta tgattagtgt agggagcttt tcgtatgctc attcaggaag aactgattca
     2761 tcaggaggtc atcgtgataa taaaaataaa tctggattag gaagttatca ctatcattgt
     2821 ggtggttatc cagcccattt acataataat ggtggttgtc cttatactgg aggaggaagc
     2881 agttctggaa caacaacttc ggttaataat gaagaaaagc aaaagaggtc tgttggagaa
     2941 aaaggatata atcaagggta tgaagatggg tataaaggaa attatagttc atcaaattat
     3001 agtggagatt atagtgatac atatgaatca aagtactcag aaggatatga aaaaggtaag
     3061 gctaaattag aagaggaaga aaaggtggct aaagagactg gatataattt agggcttaca
     3121 ggtgctaaat ctaataatac atatgaaaaa gaagttttaa aaaatgctta tgataccgga
     3181 tattcaactg gatacaatga atataagact aaaaagatag aagaatataa agctaaaggg
     3241 atagaggata gcaataaaga taaagaaaaa atgacatttg aagaaaacat agattctgaa
     3301 tttatagatg cttataataa tgcttatgac gaaatacaag aacaattaaa aaatgattac
     3361 actactcaag gatttgagag tgctatcaaa ggagaaagat ttgacacatc aactataggt
     3421 aatgttaaat atgctaattg gtttaaagaa ggatatgata atggtaaggt taagttacct
     3481 aaagcaaaag aaagtgtata taatcaaggt tacaatgaag aagattttag tataccatat
     3541 gaaatgaagt ctattgaaac taagttaaaa ggtgtatatg atgaaggttt agaaaaaaga
     3601 gaagaagaga aaagtagaaa tgctacttat ggggttggag ctggaacagc agctgtagtt
     3661 gctggagtag tatataaaaa taaaaaacgt aaaaagatat agatgctaga tataccaatg
     3721 atgacaagtg tataacaaat ataattttaa gtataaatat ttaaaatata gaagggaagg
     3781 ttatatctat gggatttaac tttaggaaaa gcataaatct aggaggagga ctcaaattaa
     3841 atttaggtaa gaagagtgta ggtataagtg ctggtgttaa aggtgctaga gtgtcagtta
     3901 attcaaaagg aagaaaatca gctacattga gtatacctgg aactggaata tcatatacaa
     3961 aaactagtac aaagaaacgt aaacctaaat aaatatttga tatactaaga aatagactgt
     4021 tacaaatagt ctatttttta gtatatagat ttaaaaagtg acaaaatttt gaaatataaa
     4081 ctaatttcat aaaggaaaat attcttttat gtagaattat aatatttaag taatattata
     4141 aaatttatag gaggatttca ttatgaaatt aagcaaaaca ctattggcaa tagggatgtc
     4201 tgtttcatta ttgattgcta gttgtccaac atcaaacgct cttagttcta ttaattcaat
     4261 aaaaggtaca aataagtatg aaacagcagg tttaatagca gataaacaaa attatgatat
     4321 tgcaatactt attaattctg aaaatggatt atctgatgga ttgtcagcaa gtggattgtc
     4381 aggagctaaa aatgccccaa tattgttaac taagaaagat acaatccctt cagaaacatt
     4441 aaaaagatta gaaaaggtaa aagaagtata tctaattgga ggaaaaaatt cgataggagc
     4501 atcagttcag aaaatattaa gtgataaaaa aataagcata aatagaataa gtggaactga
     4561 tagaatagat acaagtcata aagttgcaaa agaaatagga agagtaagta cggttaaaaa
     4621 agtaatatta actaatgctt ttaaaggcga accagatgct atgagtgttg cttctatagc
     4681 agttagagat aaatctccaa tagtattaac tgatggaaat actacaagtt ttgctacaga
     4741 tggattagaa acttatgcaa ttggtggaaa agtcagtatg agtgataagt taataaatga
     4801 aactaaagca acaagaatag gtggagtaga tagatacgat acaaataaac aaataataaa
     4861 taaattttat aatggctcaa aagaatttta catagcaagt gggactgatt tagtttattc
     4921 tttagttggt tctacaattg cacaaaaaac acctatagtg ttagttggaa atggaagtaa
     4981 taagagtata ttaaaaaatg caagtaaaat aactgctatt ggaaatattg atgatgttat
     5041 tataaaacaa tgtttaaata taactaataa tgttggggat tcaaatacag gtattgtaaa
     5101 agatactaat tcaaataata caaacaaacc aagtaatgag cctagctcta ataatcctgg
     5161 attaactgat aaaagtacag tatatgtaac tcctaatgga aaatcatatc atctaacaaa
     5221 aaattgttct acactaaaaa gaagtaaaac aataaataaa gtaacattaa aacaagctaa
     5281 gtcacaaggt aaaatagacc cttgtaatgt ttgtgtaaaa taatgtatta gccaaatata
     5341 tattaaaaaa gacaattaat taagattgtc ttttttattg aagaaatata aatttatatt
     5401 atgtattatt aaaaagaaaa ttattaatta taaaaaataa taataaagac gataagtatt
     5461 aaaattttat gtttccaata tttatgatat aattaatatg gcaaggaata aatatttgaa
     5521 aaagctatga gtggtgtttc catggaagtt tcttcacttt cttatgagag gaggtgaaaa
     5581 ctatggaaaa tctaatgatg agtattataa ctggtgttat agccagttac atttacgata
     5641 aattaaagag ccactctgac ca
//
LOCUS       DAEPOZ010000228         5650 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.228, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000228 DAEPOZ010000000
VERSION     DAEPOZ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5650
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.228"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(61..840)
                     /locus_tag="KR440_003152"
     CDS             complement(61..840)
                     /locus_tag="KR440_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBH0700161.1"
                     /translation="MKKAIWGICICLLSIVGVYTYNHNTHNQNSSKETYTLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQILDILKKNNVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYGSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDTIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(890..1372)
                     /locus_tag="KR440_003153"
     CDS             complement(890..1372)
                     /locus_tag="KR440_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBH0700162.1"
                     /translation="MDTKKYKDKLLKEREKLTNLVVDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDSALSKIENGTYGFCDLCHNQI
                     DLERLDILPDTNLCNNCAKKENALLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGTLRD
                     "
     gene            complement(1556..2977)
                     /locus_tag="KR440_003154"
     CDS             complement(1556..2977)
                     /locus_tag="KR440_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBH0700163.1"
                     /translation="MVSFLGSIVVLIVGYFIYGTFVEKVFGINDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGIVVAI
                     ILGLLFFKKAKDIKEQNAHKLAS"
     gene            complement(3123..3893)
                     /locus_tag="KR440_003155"
     CDS             complement(3123..3893)
                     /locus_tag="KR440_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0700164.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSSIEVVNEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYENYAVDAFSLDVFDYVLKP
                     YSEERIISMLNKLEKSEMSDIELSNSNSNLYKYKKETVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKEFRLLMHI"
     gene            complement(3898..5100)
                     /locus_tag="KR440_003156"
     CDS             complement(3898..5100)
                     /locus_tag="KR440_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBH0700165.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIVGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRTDNGT
                     LIVFYVKE"
BASE COUNT         1868 a          995 c          571 g         2216 t
ORIGIN      
        1 aagattaatg atgtctgtac tttaaactta caagcaccac taatcttcaa acaaatattt
       61 ttacttttgc acttgcttat ttttatctaa atgttgtata agttcagata ctgttacgaa
      121 tttatatcct ctctctttta atttaggaat tactacttct aaagcttgta tagtttgtga
      181 tttatcattt cttttagtgt tataatcatg taaaagtatt atattaccat tttggacctt
      241 tgtaagaata gtatttataa ttgtatctac accagggtta gaccaatctc tagggtctaa
      301 gtttgaccaa agaattatat tcatgccttt agaaactata atatcacaga cacttttact
      361 catggctcta tatggaggtc taaatatctt tggttctact cctattacac tttttataat
      421 attttgtgta tctgttattt ctttttcaac actaccataa ctattttttg caacatttat
      481 atgtgtaaat gtatgattcc ctatttcatg cccctcatca tattgccttt ttaataattc
      541 tttgttatat tctacttttt ctccaactac aaaaaaagtt gcttttacat tatttttctt
      601 taatatatca agtatttgtg gtgtaaaatc ctcatctgga ccatcatcaa aagtcaatgc
      661 tagaatcttt tcatcctcat ttcctttttt tattattaca tcttcataat cgtcttttat
      721 attgtcattt aaagtatagg tttcttttga actattttga ttatgtgtat tatggttata
      781 tgtatataca cctactatgg ataataaaca tatacatata ccccatattg ctttcttcat
      841 gtcattacct cttttctaat tcattctaac aatatatgat gtcaggcttc taatctctca
      901 atgttccatt tttatttaaa tcagttaaat ttgttaggtc ttctgaataa aagttagaat
      961 cttctacatc aggattttgc attgcatcac ctaataaagc attttctttt tttgcacaat
     1021 tgttacataa atttgtatca ggtaatatat ctaatctttc taaatctatt tgattgtgac
     1081 ataaatcaca aaacccatat gttccatttt ctattttaga taatgctgaa tctatttgat
     1141 acagtcttcc ttcttcatga tttattaaac tattttgcat attttgcata tataaatccg
     1201 ttcccatatc tccaatatga ttgtcataac tagataattc tcctgatgaa tatttttcac
     1261 tagtatgttt agtagtgtct ccaaacaagg tattatcttt catatctacg actaaattag
     1321 ttaacttttc tctttctttt aataatttat ctttgtattt tttagtatcc attttcaaca
     1381 cctctaattt taatatttat atcataaaaa tagtatgtgg aaaactaaat taaaaataca
     1441 ataaaaaaca agatttgatt ttttcaaaaa aaattgtagg taaacttaaa attcacctac
     1501 aaattcttta catatatttt taatataatg tttttagttt ttgataataa ttacattaac
     1561 ttgctaattt atgagcattt tgctctttta tatccttagc ttttttaaaa aataaaagtc
     1621 caaggattat tgcaactaca atacctattc cattagatat attaactgga agtctaaatc
     1681 cttcttttgc ctgcattata taactaaatg ttactacagt cataaaaagt gctggcaatg
     1741 tagttatgta atgattttta tttgctttga ctaaatatgc agaaccagtc cacaacatta
     1801 tcatagcaag tgtttgattt gcccaagaga aatatctcca aattatatta aaatctataa
     1861 atgtaagggc aacccctaca gcaaataatg gcaaagctat taaaaatcta tttttaactg
     1921 catcttgatt ataattcatt gcatcagcta ttgttaatct agcacttcta aatgctgtat
     1981 cccctgaagt tataggacat gctacaacac caagtatggc caaagcgcct ccaataggtc
     2041 ccattaatcc aacagatact gtatttacaa cagttgctgc tccaccatca ttaaatatta
     2101 cattaagctg aggtatacca tggaaatatg acatagctgc tgctgcccaa actaaagcta
     2161 ttataccttc tgcaaccatt gacccataaa atactggtct tgcctcactc tctttctcaa
     2221 cacatctcgc cataagtggt gattgagttg catggaaacc tgatattgct ccacatgcta
     2281 ttgttataaa taagtatggg aataaaggtg ttccatttgg attcatattt tgtaaattta
     2341 tatttggtat gttatacccc tttattatta atcctccagc tacacctact gccataatta
     2401 ataatgctgc tccaaataaa ggatatattt taccaatcaa tttatctatt ggtaaaacag
     2461 tagccataat ataataaagt attatcactc caacccatat ttgtgcgttt acacctgtca
     2521 atgtctttaa taatcctgct ggacctgtta caaatacaac tccaaccaat actaaaagaa
     2581 taacactaaa tattctcatt gctatttttg caccatttcc tagataaaga cctaccaact
     2641 ctgatgcaga tgtacctttg ttcttcattg ataaatatcc tgaagcaaaa tcgtgtactc
     2701 cacctgcaaa gattgttcca aacacaatcc acaaaaatgc tgatggtcca aataacgccc
     2761 cttgtatagc tccaaatatc ggacctaacc ctgctatatt tagaaactga attaaaaaga
     2821 ttctcttcca tttcattggc acataatcta ctccatccct acaagctata gctggagttt
     2881 ggtttttgtc attgattcca aaaacctttt ctacaaaagt accatagata aaataaccta
     2941 ctataagtac tactattgaa cctaaaaagc ttaccataaa ttcatcccct tagtttttat
     3001 ataattgctt tataaatttt tatgagttta agaaaactat ttccagttca aatagtaata
     3061 cttttttatg ctaatataaa tattaattac attaagtgca tatttaagta catgaaatga
     3121 ctttatatat gcattaacag cctaaattct tttactttgc ttctgcttac agttacttca
     3181 tagtcactat tttttaattt caatatgtat gtattgttaa accatggtat aatttctttt
     3241 actttggtta aatttacgat ataagaccta tgtgttctaa aaaatgtttt atcatttata
     3301 agattctcta cttctgatat gccttctttt aatataaact tttctttctc agtatatata
     3361 aatgtctgcc tttcattagc ttcacaataa tatatatcat caatatctat tacaaccaat
     3421 ttatcacctt tccaaaggct aattttatgt gttatttctt ctatctcctg atttactgtc
     3481 tcttttttat atttatataa attactattg ctattagaca gttcaatatc actcatctca
     3541 cttttttcta atttattcaa catagaaata attctctctt cagaatatgg cttcagtaca
     3601 taatcaaata catctaaaga aaaagcatct actgcataat tttcatacgc tgttataaaa
     3661 acaattttag gccttgattt aaactgattt aatgtttttg caagaagcat tccatcgaga
     3721 tgaggtatat ttatatccaa aaaaattaca tctattttat tttcctgaat aaagtctaac
     3781 acttctatac cattagtaaa ttcatttact acctctatgc tacttttatt ctcaataaaa
     3841 tatcttaatt cctttcttgc cggaaattca tcttccacta ttatagctct catacttcta
     3901 ctcctttaca taaaatacta tcaaagtccc attgtctgtt cttcttatat taagccctct
     3961 tccatacatc agtttaagcc ttaaatgtac attataaagt ccaatcttat tttctggcat
     4021 attatcttga tatacattat ttataatttc ctcacttata cctataccat tatcctctat
     4081 tgatattctc acagtatttt ctaatgaata aggtaacttt tttatggata ttttcactac
     4141 accagctcta ccactttcta aaataccatg tattatcgca ttttccacta atggttgtat
     4201 aatcaaactt ggaatcttta tatttacatc ttctatatca taagatacag ttagtaattc
     4261 tccaaaccta gctttttcta tctctacaaa agatttaact tgttcaatct ctttatttat
     4321 atcaattaag ttatcactat attcaagatt atatcttaga taagttgata aatttattat
     4381 taactctcta gccttatctg gactaaatct tataaatgaa gctattgtgt ttagggaatt
     4441 aaacaaaaaa tgcggattaa tttgtctttg aagtgctttt atttctgctt tatttctcgc
     4501 ttctatatct tttttatctt tctcaatccc ctctactata gatataacaa atcctattcc
     4561 aatctgtcca acaattaaag gtaaatatat cccaacaatt atttgaattg ctaaagaatg
     4621 tggatatgat attaagtata taagtagtat agtaaaactt tcagaaatca tacctaccaa
     4681 tataccatac attactctat gttgctttgg tatcctctta tatagaaatc ctgataatac
     4741 acctgctagt atacttgata aaagacatgg aacagatgtt atccccccaa tatccataaa
     4801 atatctatga atccctgaaa aaactcctgc tgtaatacct accattggac caaataaaat
     4861 gccacctgat actatagata ttattctagt gtttactatt gaccccatat aatttatacc
     4921 actataagtt ccaaatatgg ctaaaaaagt aaaaactaat gctatacaga ccaagtcatt
     4981 aaaactatat tcttcctttt gaaatatctg cttaaatatt tttaactttg ttattagaag
     5041 aaacagtact acaatgaggc ttgtcttttc tattaatgcg attaaaattg atgttttcat
     5101 attccacctt taaaaaataa atttttacac actctaaatt tattatgttt gttgtaactt
     5161 atatatttga agtaatccta tctcgacatt ttccacattt aaccttttaa ttaaataaaa
     5221 aatggatata cttctattaa caactaatac tagctgacta acacaagctt gtaataactt
     5281 gtagttatca ttaactctac aattctagta tttaatttta tactttagaa atctcttttc
     5341 ttagaagtaa tactttcgaa tattatataa tacaatgtta tgtagattct tactcataca
     5401 acatatttct ttaaaaattt tatactattc tttgttcaaa tatctcattt gatttaattg
     5461 tgctaaacac acattatcat ctctctgtta tatatttatt atatagccgt atatcgataa
     5521 agtaaataaa tcctatattt aaaatcatat gtttgatagt aaatcattat aatgtatatt
     5581 taccataata atttgccaac cttgaaagtc acttctataa ttagaagtta aataattctt
     5641 ctaaaaaaaa
//
LOCUS       DAEPOZ010000229         5646 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.229, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000229 DAEPOZ010000000
VERSION     DAEPOZ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5646
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.229"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            948..2162
                     /locus_tag="KR440_003157"
     CDS             948..2162
                     /locus_tag="KR440_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HBH0700166.1"
                     /translation="MGEKMQSVICKIFDNKLLISNDLSEQDDVLYEVMDKFIDDIEYI
                     SKLGDEQIKSMLMEVNKFEEDSTEFELYMKNLCYLKQSYFIDTNDKSEINSIDIDILK
                     NVKIEDLVDITNIKLERNLNINIEHISKAVNKIYEENEKWKEYNIQNTMSKYIGIPES
                     EVIFISNLSQYVSFLSELDNAGDYVSRGQKDCMYELLPSLHRKYKKDYGIHSDEYEEA
                     FKQKIIYYDKEIKNRSSEELRAEGQHFGLPTEYLDFTEAHLISLLFAIEDYKYTKQHS
                     IVYFIDSISYNTDTIKRSEKLVNYSDTSAIDNCSKFSSRSFFIKVGNSNERIHFQKGC
                     FLKVSSEDKGEFKEKLKKYCKVVVINKDAKKEILKELFNLGITFENIYPDKDNVVKSI
                     KFYYDEIVGGNL"
     gene            2162..3454
                     /locus_tag="KR440_003158"
     CDS             2162..3454
                     /locus_tag="KR440_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBH0700167.1"
                     /translation="MREQFKNSIKNIKSNRPLVSVQDIVNFIDKGEKYLKLSIDEIDE
                     MEILQGIVMSPDYQRTYKSSNKEESSIIESLLIGIPIPEIFLVKIGVNDMQIRHVMDG
                     QHRLNAIYRYVKNKYPLKNLDILGNDPLYDNKRFSELEKKDKIKILGSHLSILEFDGF
                     DNPEIEIELFKRYNRNTKPLEMQEIEMATYFSETSKYISKFINTLIKDKYKDEIDKSE
                     YKYANYKSDLLRIYNITKSRNDKQKNHQEICIIFSILENGLQENIRDGVTASKKFLEY
                     KSIQYKNNESEKLELLKDKFDEFNRMILKISEKIEYPFSTSLISDDKQRQSKYLIGVS
                     IVLASICYYYDIDLENNYLIQDIKKIISCSPIADIEYKASSTNMKNMILYLFIKNKIQ
                     DNKFESLNLKRDKIKEINTLVMNNDGYLNEQTRLLEIY"
     gene            complement(3681..3839)
                     /locus_tag="KR440_003159"
     CDS             complement(3681..3839)
                     /locus_tag="KR440_003159"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBH0700168.1"
                     /translation="MEMYYLRKLDTYEQLVKTIEDYIYYYNNFRYQKRLNSMSPLEFR
                     KYLNTEIA"
     gene            complement(3896..4285)
                     /locus_tag="KR440_003160"
     CDS             complement(3896..4285)
                     /locus_tag="KR440_003160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HBH0700169.1"
                     /translation="MRAVGLSSVCRKKKFSYVKCTPEVIAENVLSRKFSADKTSQKWL
                     TGVTEFKLTNGMKAYLSAILDLDDRSIVSYVIGKSNNNNLVLETFDKAIELYPNAKPI
                     FHNDKSFQYTSKVFKSKLLTQGMIQSM"
     gene            complement(4297..4757)
                     /locus_tag="KR440_003161"
                     /pseudo
     CDS             complement(4297..4757)
                     /locus_tag="KR440_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888992.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5186..>5646
                     /locus_tag="KR440_003162"
     CDS             5186..>5646
                     /locus_tag="KR440_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700170.1"
                     /translation="MITKIKKTYIYLKEIQKIYKINEHKELVHIIKKLINDNKIKPIK
                     TSDLTPQHEQLYTKYRIIKQETEEDKKILEEINYKICDKLSIDYYRKNLVHYKNNRVA
                     IMDLNSYLIKKSDNLSKKISINERSYEIFKDEKFISSKVGKEVLKNLKIDL"
BASE COUNT         2323 a          604 c          761 g         1958 t
ORIGIN      
        1 attaggataa tagttagtaa atgatatgat tgagagaata gatataataa actaatctag
       61 tcctgtattg caatgtttat taaagaatat atttttaaag atccaagtac tgcatctctt
      121 ccagaattga gttggttaat agttttttga tgctatgcaa tttataataa aaagtataat
      181 gatttagttg atgaatcatc agaatttata ccctcaaaga ttaatcagat tgcaataaaa
      241 ataatgatat attctatagt tataatatct attttctttg caacccctca tacaatgaat
      301 ataaatatat caaacttgga tatgggtata attctcattt cagttttatt tatacagttt
      361 ttatctaaat taattttgta tgtatatttt gataaaaggg agatatgtaa ctaagtggta
      421 atattggtga aaacaaaaat taaagaatat agaaaaaaac tccttatgac acaaaatgaa
      481 cttgctaaat cagttagggt aaggtgtgag actattgtac acttggaaaa tataatcctt
      541 ctttaaagtt agtgatagat attgctaaag tattcaatac tacagtagaa aatttgtctg
      601 aatttattga agaaggatag tggatagatt ataaatagtt ataagatggt taatgaaata
      661 tagttactta attattaagt gtgttagaaa tatttatatg taacacaatg tgtagttaag
      721 attaccttat ctcatctaag actttaattt ttcttcccat aaagagggta tatcaatttt
      781 ctagtgtggt agcccatttt ttattgattt aaatttataa tatatattaa tagatgatac
      841 ttaaaaattt tataaaatat ctaagttgtc ataaataaaa cgatatatct aaccgaatat
      901 ggtaaaattt gttataatat agataagtta gagtaaaaat aattttaatg ggggagaaaa
      961 tgcagtcagt aatatgtaaa atttttgata ataaattact aattagtaat gatttaagtg
     1021 agcaggatga tgtattatat gaggtaatgg ataaatttat agatgatatt gaatatattt
     1081 caaaattagg agatgagcaa attaaaagta tgcttatgga ggtaaataaa tttgaagaag
     1141 attcaactga atttgaacta tatatgaaaa acttatgcta tttaaagcaa tcatatttta
     1201 tagatactaa tgataaaagt gaaataaatt ctatagatat agatatatta aaaaatgtta
     1261 aaatagagga cttagttgat ataactaata ttaaattaga acgaaattta aatataaata
     1321 tagaacatat atctaaagct gtaaataaaa tatatgaaga aaatgaaaag tggaaagagt
     1381 ataatataca aaatactatg tctaaatata taggtatacc tgaatcagaa gttatattta
     1441 tatcaaattt gagtcagtat gtttcatttt tatctgaact agataacgca ggcgactatg
     1501 tatctagagg gcaaaaagat tgtatgtatg aattattacc atcattacat agaaaatata
     1561 aaaaagacta tggtatacat tcagatgaat acgaggaagc attcaaacaa aaaataattt
     1621 attatgataa agaaataaaa aatagatcga gtgaagaatt aagagctgaa gggcaacatt
     1681 ttggactacc aacagaatat ttagatttca cagaagctca tttaatatca ttattatttg
     1741 caatagaaga ctataaatat acaaaacagc attcaattgt gtactttata gactctattt
     1801 cttataatac tgacactata aaaagatctg agaagttagt aaattattca gatacatctg
     1861 caatagataa ttgtagtaaa ttttcatcaa gatcattttt tataaaggta gggaattcaa
     1921 atgaaagaat acattttcaa aaaggatgtt tcctaaaagt atcatcagaa gataaaggcg
     1981 aatttaagga aaaattaaaa aagtattgta aagtagtagt tattaataaa gatgctaaaa
     2041 aagaaatatt aaaagaatta tttaatttag gtattacatt tgaaaatatt tatccagata
     2101 aagataatgt agttaaatct ataaaattct attatgatga gatagtaggg ggaaatttat
     2161 aatgagagaa caatttaaaa attctataaa aaatattaaa tcaaacagac ctcttgtatc
     2221 agtccaagat attgtgaatt ttattgataa aggagaaaag tacttgaaat tatcaataga
     2281 tgaaatagat gaaatggaaa ttttacaagg aatagttatg agtcctgatt atcaacgtac
     2341 atataaatca tcaaacaagg aagaaagttc tataattgaa tctcttttaa ttggaatacc
     2401 aatacctgaa atatttcttg taaaaatagg tgtaaatgat atgcaaataa gacatgtaat
     2461 ggatgggcag catcgtttga atgctatata taggtatgtt aagaataaat atccacttaa
     2521 aaatttagat atattaggca atgacccact atatgataat aaaaggtttt cagaattaga
     2581 gaaaaaagat aaaattaaaa tattaggaag tcatttatca attctagaat ttgatggttt
     2641 tgataatcca gaaatagaaa tagaattatt taaaagatat aacagaaata caaaaccatt
     2701 agagatgcaa gaaatcgaga tggcaacata tttttcagaa actagtaagt atataagtaa
     2761 atttataaat actctaataa aagataaata taaggatgaa atagataaat cagaatataa
     2821 atatgctaat tacaaatcag atttacttag aatatataac ataactaaat ctcgtaatga
     2881 taaacaaaaa aatcatcaag aaatatgtat aatatttagt attttagaaa atgggctaca
     2941 agaaaatatc agagatggag ttacagcctc taagaaattt ttagagtata agtcaattca
     3001 gtataaaaat aatgaaagtg aaaaattaga gttattaaaa gataagtttg atgagtttaa
     3061 taggatgata ctgaaaatat ctgagaagat agaatatcca ttctcaacaa gtttaatatc
     3121 tgatgataag caaagacaaa gtaaatactt gataggagta tctattgtgt tggcttctat
     3181 ttgttattat tatgatatag acttggaaaa taattattta atacaagata ttaaaaaaat
     3241 aatatcttgt tcacccatag cagatataga atataaggcg tcttcaacta atatgaaaaa
     3301 tatgattcta tacttattta taaagaataa aatacaagat aataaatttg aatcattaaa
     3361 tttaaaaaga gataaaatta aagaaataaa tactttagta atgaataatg atggttattt
     3421 aaatgaacag acaaggctat tagaaatata ttaacaagtt ataaaataaa tcatggtaat
     3481 gtaaaataat ttgtgctttt aaggttaata aaaatcttct ttatggcaat tatttaaata
     3541 taatcaaatg tcgtaaagga gattttttat gaacgcttta agaaaacaat tagccaaaga
     3601 aattatatca gatatctaaa aaagagcatg tcaaataaaa ccctaggtat ttaaattttt
     3661 ttagagctac ttaaaataaa ttatgctatc tctgtattta aatatttcct aaattcaagt
     3721 ggagacatag aatttaatct cttttgatat ctaaaattat tatagtaata tatataatcc
     3781 tctattgttt tgactaattg ttcgtatgta tcaagttttc ttaaatagta catttccact
     3841 tttaaaattc tccaaaatcc ttccatagga gcattatcaa tacaacgagc aactcttaca
     3901 tactttgtat catgccttgt gttaaaagtt ttgatttaaa taccttacta gtatactgga
     3961 aacttttatc attatgaaaa ataggttttg catttggata tagctctatt gctttatcaa
     4021 aagtttctaa caccaagtta ttgttatttg actttccaat aacatatgaa actatacttc
     4081 tatcgtctaa atctagaatt gcgcttaaat aggccttcat cccattggtt aatttaaatt
     4141 cagttacacc tgttaaccat ttttgagagg ttttatctgc actaaatttt ctacttaata
     4201 cattttccgc gatcacttct ggcgtacatt taacataact aaatttcttt tttctgcaaa
     4261 cagaacttaa gcctacggct ctcattagtc tgtacactct ctacaatctt taacaatttc
     4321 aactctttca tctaatgctg tttttctagt ctccataaag atttctccct tcgaggtttc
     4381 ttttatctac ttatgaggat tatactcttt accccaagct tttagtgaat aaactgatct
     4441 cattttatat ttttttgaga ttgtgtcata agatccttca ccatttagat aagattttat
     4501 agcattcaat ttaaattctt cacaatagca taatttacaa ctttgtttta ttaaaccatc
     4561 atttccaaaa gcttcataat ttcgcatcca ctctctgaat gtacgtttat ccaatattta
     4621 attcttccat tatttttatt actgtattct caccttttaa atatgattca attgcttcga
     4681 tttttttgct ctgaagatat cttagtttta gatagtctta catattttaa ctgtctccta
     4741 agatgagtat aattcattca tcatcagttc tatagaatac acctagttcc tacttactgt
     4801 aggaactagg actatatgaa acccattcat agattgtagt ttcttctgtt gcattatagc
     4861 gcagaatagt aaattcatgt gaaattttat tttgaattta ctatttatat ataaatgaag
     4921 agtggtctta ttttcaatta aaatttggaa caattttggg ttgacatcta caattaagga
     4981 tttaagtttt tgttatacta ataaataaaa acagagattc aaaatatata aataatactt
     5041 aaactagtgc tgatagattt tctttaagtt ctagtttttt tattgtcatt atctaatatg
     5101 tactatttta tgtacagtca atataatctg aaattgtaaa atggtgtata atatagttat
     5161 aagtaaagaa gggatttaaa catgtatgat tacaaaaata aaaaagactt atatatacct
     5221 taaggaaata caaaaaatat acaaaatcaa tgagcataaa gaactagtac acataataaa
     5281 aaaactaata aatgataata aaataaaacc aataaaaacc tcagatctaa cacctcagca
     5341 tgagcaatta tatacaaagt atagaataat aaaacaagaa acagaagaag ataaaaaaat
     5401 attagaagaa ataaattaca aaatatgtga taaattaagt atagattatt atagaaaaaa
     5461 cttagtacac tataaaaata atagagttgc aataatggat ttaaatagtt atcttattaa
     5521 aaaatctgat aatctatcaa agaaaatatc gattaacgaa agatcttatg aaatatttaa
     5581 ggatgagaaa ttcataagta gtaaagtagg aaaagaagta ctaaaaaact taaaaataga
     5641 tctaat
//
LOCUS       DAEPOZ010000230         5644 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.230, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000230 DAEPOZ010000000
VERSION     DAEPOZ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.230"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..447
                     /locus_tag="KR440_003163"
     CDS             <1..447
                     /locus_tag="KR440_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0700171.1"
                     /translation="ILDIIINEKSEICLKKGIKFTCSINFSKVNFVKPIDVSSIFSNI
                     LDNAIEACDKIVDENINKYIRIKGTITKSFFVLKCENSKLNQLTFNKNILLTNKMDKF
                     VHGIGIQSIKSSLQKYNGELLFENSIDKFILNIYIPLEQDTDSWGD"
     gene            688..1377
                     /locus_tag="KR440_003164"
     CDS             688..1377
                     /locus_tag="KR440_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700172.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMAGCMSPVSDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITDSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYIK
                     FIYNLFKSLTNTNLTEKEIFSMVEEEFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            1596..3968
                     /locus_tag="KR440_003165"
     CDS             1596..3968
                     /locus_tag="KR440_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HBH0700173.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAEGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            3983..4885
                     /locus_tag="KR440_003166"
     CDS             3983..4885
                     /locus_tag="KR440_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBH0700174.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRTQINNKCDLCGECIKVCLKDAICIMT
                     EKMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            4905..5456
                     /locus_tag="KR440_003167"
     CDS             4905..5456
                     /locus_tag="KR440_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0700175.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSYVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
BASE COUNT         2268 a          563 c         1050 g         1763 t
ORIGIN      
        1 atattagaca ttataatcaa cgaaaagagt gaaatatgtt taaaaaaagg aattaaattt
       61 acttgctcta taaatttttc aaaagtaaat tttgtaaaac ctatagatgt gtcaagcata
      121 ttttctaata ttttggataa tgcaatagaa gcatgtgata aaatagtgga tgaaaatatt
      181 aataaatata ttagaattaa aggaactata acaaagtcat tttttgtact taaatgtgaa
      241 aatagtaaac taaatcagct tacttttaat aaaaatatat tgttaacaaa caaaatggat
      301 aagttcgtac atggaatagg tattcagagt attaaatctt ctttacaaaa atataatgga
      361 gaattattat ttgaaaatag tatcgataaa tttatactaa atatatatat accattggag
      421 caagatactg acagttgggg cgattaagtt accagttggg acatatgtat tgatacaaaa
      481 aaataaggat gttatctttt aataacaaaa gataacatcc ttatttctgt attaaggagc
      541 ttataatttt actaatagta agttataaga attggatagg aaggtgatat gtattgaata
      601 ataaatcttt aatataaaga aaatataaat atattctaat gcaatgaata atactttaga
      661 gcggttagtg tgatgggagg attgtgtatg aaagtaaaaa ggaatataaa tagagtggca
      721 ataattgtgg ctttaaatct tagcttagca ttgatggcag gatgtatgtc tcctgtatct
      781 gataagaact taagtagctc agaacaaatt agtaaagatt cattgaaaga acaagttgaa
      841 gataaaaaag atgaatctat cgagctattt aataattact cacaaaagat taatgatgcg
      901 aaaggtagag aagaagtaaa aaaagattta tctcaaagat tttcgaaagc tataatagac
      961 agtggttata gtattactga ctcaaaatca gatggtagct ttgtagtatc tataaatggt
     1021 gctgaaaaac cacctgaaaa aaatattcga agtttattct attctatgtc tgaagacgat
     1081 aaagaaggaa aagtaacaat agatattctc tgtgctaaag agtatccaga agatgataag
     1141 ttatctgaga gtgataaata tattaaattt atatacaatt tatttaaatc attgactaat
     1201 acgaacttaa ctgagaaaga aattttttct atggttgaag aagaatttaa taagggagta
     1261 ggtgttgtgg agcttcctta tatgaaggat atacatgttg aagttaacaa agttaaacaa
     1321 tcaactaagg ttttaaaact aagtctagta tacaaatttg atatttcaaa tcattagtat
     1381 attagaaata actataacaa tatagaaaat atatcataga aaattttttt agattttata
     1441 aatttaacaa aaaaagtatt aatatatagg tgttcaaggt agatgaaaaa aaaagttatt
     1501 ttgcttccat tttatgctaa aggacagtat aatataatta aagtatgtta taacatacaa
     1561 aaatgtagta tatattatcc aaagggggat tttaaatgag tcaaactaat agaatagaag
     1621 catttagaca agaatatatt aattctaaac ctatgatatg ttgtgaaagg gctagaattt
     1681 ttacagaatc acataaaaaa actgagggag aagctatctg tataagaaga gctaaagctt
     1741 ttttggagac atgtaaagaa cttcctataa aaatctttga aaatgaatta atagtaggta
     1801 cagcaggaaa atttagaaga acagggatat tgacaccaga gttttcttgg caatgggtag
     1861 acaaagaaat ggatactttt gataaaagaa ctcaagaccc atatgtaata tcaaaagaac
     1921 aaatagaatt tataagaaaa gaaatatttc catattggaa gggaaaatct ttggaagaag
     1981 ttttcttagc aagaatccca gaagatacag ctaagatatt agtagataca ggaataatag
     2041 ataatgattc taagtggaga caggcagttg gtgaagtaac accagattat caagatatat
     2101 tgtttgtaaa aggatataaa gggattaagg aagatgcaga taaaaaaatt aaagaattag
     2161 atatatcagt ttcagaaaat attgaaaaga tagattttta caaatctgtt tctatagttg
     2221 cagagggtat tatgacattg gcacaaagat attcaaatct tgcaaaagag atgagtaagc
     2281 aagaaacaga tgaaaagaga aagttagaac ttataaaaat ttcagagata tgtatgaatg
     2341 taccagcaaa ccctccaact aatttttatg aagcaataca atttgtatgg tttgttcaat
     2401 taggtggtat tctatcagaa aatccattgg cattaaattt aggtagattt gaccaatata
     2461 tgtatccata ttatgaaaat gatgctagag aaggtaaaat aactgaatca gaagctcaag
     2521 aattgataga agcactttgg attaagttat cagaatgggt atggactata tcagcaaata
     2581 cagcaaatta ctttgcagga tataatcaat tccaaaatct tactgttggt ggtaaaaaga
     2641 gaaatggtac tgatggaacg aatgatatct catatatgtg tttaaaggct acagaaagtg
     2701 taaaaactca ccagccagga ttaagtgtta gagtgagtca aggagcacca gataatttcg
     2761 taatggcagt tgctaaattg gtaaaacaag gaactggttt ccctgctata cacagcgata
     2821 gtgcaggggc acaaatgtta ttacaagatg gatatgatgc agaagatgct agagattgga
     2881 gtaactgtgg ttgtgtagtt cctcatttta gaaaaacagg acagtggact tcagccgtaa
     2941 atatcaattt tgcagcagct ttagaatatg ctatgaatga aggtaagagt agattaactg
     3001 gagaaaaaat ggggttagat acaaaaaata tcacagaatt tacttctttt gaagaactta
     3061 aggatgagtt tttaaagcaa ttagcatatc ttgtaaaaag ttctgttata ggaactactg
     3121 ttgctcaaca aattcataaa gaaatggtac caagaccatt tttatctact tgtgtagatg
     3181 gatgtttgga taagggagtt gatttaagta aaggaggagc aaaatataat ataggtcctg
     3241 tattaactgg tatagggtta ggtgtagttt caaattcatt ggcagctata aaaaaattag
     3301 tatttgagga taaggtgact acgctagaag aattaacaaa agcactaaat aatgattggg
     3361 aaggttatga agaattaaga aaacttgcgt tggatgttcc taagtatgga aatgataatg
     3421 attatgtaga ttcattagca attgaagttt ctgattttta ttatactgaa actagaaaat
     3481 acaaggatat ttttggctct aaatttaata gtgcatttat gggtatatca aactatgtac
     3541 caactggaaa aatcgtaggt gcaactcctt gtggaagaaa agcgacaaaa cctttgacag
     3601 agggagtttc tccgtttgtt ggtactgata caacaagtcc actagcagcc atgaaatctg
     3661 catcaaaaat aaatcatgat gttcatactg gtggaacact tctaaattta agactgaatc
     3721 aagatttggt ggaaactgaa agaggactta gaaatctaac atctatgatt aaatcatatt
     3781 ttgctttagg aggattccat gtacaattta atacgatatc aaatgatact ctattaaaag
     3841 ctcaagaaaa tccagaagaa tataaagatt tattagtgag agttgctgga tatagtactc
     3901 aatttgttaa tttatcaaga gaaatgcaag atgctataat agctagaaat tcacatagca
     3961 atttttaatt atgaggtaga ttatgagtaa aaaaggtaga gttgtaaaag tacaacattt
     4021 ttcagtaaat gatggagatg gaataaggac tactatattt ttagaaggat gtaaattaaa
     4081 atgtaagtgg tgttctaatc cagattcctg gagtaacata gtaaaattag gtgttatgaa
     4141 agataaatgt gtatcctgta atagatgtat agatgtttgt cctcaaaata taagttcact
     4201 atttgataga acgcaaataa ataataaatg tgatttatgt ggagagtgta tcaaggtatg
     4261 tcttaaagat gccatttgta taatgaccga aaaaatgagt gttgaagaaa tagtagagga
     4321 agttgaaaag gattttatat ttttctttga atctaatgga gggataactt tttctggagg
     4381 tgaacctaca cttcagattg attttttaag agaattagtt gatatttttt atgataaagg
     4441 cataaatatt gcaattgaaa cttgtggata ttttgattgg aataaggtaa atgatgtatt
     4501 tgagaaaata gaccacatat ttgtagatat aaaatctatg gatgataata ttcataaaga
     4561 atatacagga gtaagtaata aaattatatt agataatata tgtaggcttt caaagttgaa
     4621 taaatctatg gttataagag tgccaattat ttatggagta aatgatagtg aagaaaatat
     4681 aagaaatact gcattatttg tcaaacaaaa tgttccagga ggcaagatgg agttacttcc
     4741 atatcataaa tttggaatcg ataaatataa ggcattaggg cttgaagatt atatatatga
     4801 atttgatgaa atttgtaaca atcatatgtt aaaattaaaa gagatagtag aacttacagg
     4861 agtaaaaatc atagaataca aatagctatc aggaggattt taacatggat aataaatttc
     4921 ctaatgtggg agaaaattta aggttattga gacaagaaat gggcataagt ttagacaaag
     4981 cttcaaagat gacaggtgtc agtaaagcaa tgcttggaca gattgagaga ggtgaatcaa
     5041 gtccgacagt atctacttta tggaaaattt catctggatt taaaataaat tttactacac
     5101 tattaaatga aaatacaaat acctatgagg ttataaagaa agaagaagta gagcctattg
     5161 ttgagcaaaa agggaacatg aagttatatc caatataccc ttttagccct caaagaaggt
     5221 ttgaactttt tataattgag cttgaggaaa actgtactca tgtttcatct acacatagtt
     5281 atgttttaga agagtatgta ctcgtaatag aaggtaaact tgaccttaat gtggatggga
     5341 aaacttatat tttagagtct ggacattcta ttaggtttga tggaacttta gagcatatat
     5401 ataaaaattt aaataaagga aaaactatat ttcacaatat agaggtttat agataatttt
     5461 atagttatcc ttttctaaaa taaatgaaaa ttttagaaaa ggataactta tttttataat
     5521 gtcgaaaaga tttatatgta tgtgatgatg ttattgtttt aaaaaaaaca taaaagcatt
     5581 ataataatat taacaaaata gaataaagag gaagagaata tgattataaa ggtttcaatg
     5641 acag
//
LOCUS       DAEPOZ010000231         5629 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.231, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000231 DAEPOZ010000000
VERSION     DAEPOZ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5629
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.231"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            281..1705
                     /locus_tag="KR440_003168"
     CDS             281..1705
                     /locus_tag="KR440_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HBH0700176.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDSTSLEEAKAKMSDMKSASKNSEKNIEEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDALFKRLESK"
     gene            1740..3869
                     /locus_tag="KR440_003169"
     CDS             1740..3869
                     /locus_tag="KR440_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBH0700177.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFALASKWLERFDIELVRKPGLGVYLKG
                     QEKNFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIRK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDGVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNAKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMTLEVKDIVFNFYTNRLKAFIDK"
     gene            3899..4321
                     /locus_tag="KR440_003170"
     CDS             3899..4321
                     /locus_tag="KR440_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0700178.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            4340..5491
                     /locus_tag="KR440_003171"
     CDS             4340..5491
                     /locus_tag="KR440_003171"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBH0700179.1"
                     /translation="MKKAIQFGAGNIGRGFIGSLLVKSGYHVVFADVNEEILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEEILNNIEKSYIFMKNL"
BASE COUNT         2339 a          576 c         1018 g         1696 t
ORIGIN      
        1 atatccaaaa gtacatttaa taaatttatt taactgaatt attattttca tttctattaa
       61 atttaaatat tttataaaaa tagtattatt tacaaaatca tatactccaa aatactacat
      121 aaataaaatt aaacaaaaac taaaatgaca aatgtcatta tctaaagtga catagaaacg
      181 gtttcttaaa gttgttacta taaaatataa actctataat aaaagtataa caaaagggaa
      241 tcatatcgat atagaatata attcaagagg aggtaaaatt atgagtagtt ctaatcaatc
      301 gacaataaag acatctatac agcgagtagg gaaattttta agtggtatgg taatgccaaa
      361 tataggagct tttatagcat ggggtcttat aacagcatta tttataccaa caggatggat
      421 gcctaatgaa aaattgagta ctataggcga cccaatgata aaataccttt taccactatt
      481 gatagcttat actggtggta aagcaatagc aggacaaaga ggtggcgtaa taggtgcagc
      541 agcagctatg ggagtaattg ttggtgcaga tataccaatg tttatcggag caatgataat
      601 gggaccattt gcaggatggg taattaagaa gtttgataaa tttgtagatg gaaagatacc
      661 aacaggattt gaaatgttag taaataactt ttcaataggt ataataggta tgcttcttgc
      721 aatactagga ttttatgcaa ttggaccagc aatagtagca gggacagctc ttatagaatc
      781 tggagttcaa tttatagtta gcaaaagttt attgcctcta gtttcagtat ttatagaacc
      841 tggaaaagta ttattcttaa acaatgcaat aaatcatggt atattagggc ctataggtat
      901 agctgaggca aaagaagcag gtaaatctat aatgttctta ctagaaagta accctggacc
      961 aggtttagga gttctattag catattggat gtttagtaga ggaagtgtaa aacaatcagc
     1021 accaggagct gtaataatac atttctttgg tggtatccat gaaatttact tcccatatat
     1081 tttaatgaat ccagttttaa tattggctac aatggcaggt ggagcagcag gaatattgac
     1141 tttttcaata ttaggagctg ggcttgtagc agcaccatca ccaggaagta tatttgcact
     1201 tatggcacta gctccaaagg gaggattact tccagtatta gcaggagttg cagtggcaac
     1261 agttgtatca ttcttagtgg cagcaccatt tgttaagaga gcttcagcaa atcaaagtga
     1321 agaagacagt acttctttag aagaagctaa agcaaaaatg agtgatatga aatctgcaag
     1381 taagaactct gagaaaaata ttgaggaaaa acagcttgaa gtaaatgaga ttaagaagat
     1441 tgtatttgca tgtgatgcag gaatgggttc gagtgcaatg ggagcttcca gatttaaaaa
     1501 tagaataaaa aacttagact taaatataga aattacaaat tcatctgttg ataatcttcc
     1561 agatgataca caaatagtag taactcacaa tacattagtt gaaagagttg ctaagaataa
     1621 ttcaagtgtt gagatagttt caataaacaa ttttttaaat gacccaaatt tagatgcatt
     1681 atttaaaaga ttagaatcta aataaaaata aaaagtacca ctaattagga gtggataatt
     1741 tgaaaaaaaa gaagattaca tctaggcaaa aaaagataat actaatgata gtagaaaatt
     1801 caaagaaaaa cattccaata actatttctg agattgcagg gactttagag ctaagttcaa
     1861 gaactgtact tagagatatg tctggaattg aaaaatggtt tgatgaaaat gattttaact
     1921 ttgtaaaaaa accaggagtg ggattgattt tagaggaaaa tatagaaaat caaaacttta
     1981 taattgagtt gttagaagaa gaaaaaatag aaaaagaata cagcaaggaa gaaagaaatt
     2041 taattatatt atcaaagcta ctagtttcta acgagccagt aaaatcctat tattttgcaa
     2101 aaatattaaa ggtatctgaa ggggtactga ataatgattt tgccttagca agtaaatggt
     2161 tggaaagatt tgatattgag cttgttagaa aaccagggct tggtgtctac ttaaaaggtc
     2221 aagaaaaaaa ctttagagag gcatatgtaa atcttatata cgattcattc aatgaaaaag
     2281 aaatactaga tatggtcaga aatataagtg aaaatataca aacagataaa gcaatagaaa
     2341 ttttaagtga aaatagactt ttaaatttaa tggacaggtg cataataaga aaagttgaag
     2401 ctacattaac aaagaagctt tcagatttag atgtaaattt agctgatagt gcatatatag
     2461 ggcttgtagt tcatatttca ttagccttac agaggataaa aaatggtgaa aatataacta
     2521 tggataaaga gtttctaaaa gaattatcta taacagaaga gtttaaatta gctaaagaaa
     2581 tcgtaaaagg tatggaaaca gattttaaca tggatatacc agtagatgag gttggatata
     2641 taactatgca tataagaggt gcaaagcaaa ggtcatcttc aaaccataag gctttaaact
     2701 tagatgatat agaaataatg gaaattacaa ataagatgat agatttggcg gaagatgaat
     2761 ttaaaataag tttaaaaaat gacgagaggt tatttaaaga cctagcaaat cacttagggc
     2821 cttctataaa tagattgaat atgggattag aaataagaaa tcctctttta gatgaaataa
     2881 aaagcaagta ttcatatgcc tatgatggag ttgaaaagat ttctagaatt ataaaagata
     2941 aactaaatat aaattcaata cctgaatcag aaataggata tattgcaatg cactttgcat
     3001 cagccataga aaaaaatctt atgatgaata ctaatataaa tattgttgtg gcatgtccca
     3061 ctggaatagg tacttcaaga ttcttatcaa ctaagattga aaataaattt cctaatttaa
     3121 atatacttga aaccatatca gctataaaca tagatgaaga gtatttaaaa gaaaaagatg
     3181 ttgatttaat agtgtctaca gttgagttaa atacaagttt aaattatata tgtgtaggtc
     3241 catttatgag tttagatgat gaacagataa tcaaggaaaa aataaaatct atagctcaaa
     3301 ataaattaat caatttaaat gcgaaaaatg atactaaaag taagaataaa gtatatgaac
     3361 agataacaga aagtatgaat ataggaaaag atattcttca atttttagag gaaattagat
     3421 ttgaaaaatt tgaaagtaaa gatttaagtg agcttattga agactcatca aaaatatttg
     3481 caaaatctag tgaagatata atctctataa aagaatcgtt aaaagaaaga ttaaaaatct
     3541 caattccata tatagaagaa tcaaaaattc tattgttaca ttgtatgagt gaaagaatag
     3601 atattatgaa gttatcaata ataaaactag agaatagaat cgtgctagat tcaaatgaag
     3661 aaatcgataa tgtagttttt atgttacttc ctaaaaattc accaagttat caaagacaaa
     3721 taatgagtga aataagtggt tcattaatag acaatataat ttttactaat aagataaata
     3781 aattctctat agaagagatg acattagagg taaaagatat agtttttaat ttttatacaa
     3841 acagattaaa agcatttata gataaataaa attaatatta aaaaggaagg ttgatactat
     3901 gagtaaagta ttgaatgaga ataatatatt tttagggtta gatagtgttt caaaggaaga
     3961 agctataact ttagcaggaa gaaaattagt tgaaaatgga tatgtaaaag aagaatatat
     4021 accagcaatg ttagaaagag aaaaagtaat gactacatat atgggaatgg gagttgctat
     4081 tccacatggt gtaaatgaag caaaaaaaga aatattatct tctggaatag ttatattaca
     4141 attccctaat ggtatagatt ttgatggaga aaaagcatat ttattaatag gaatagctgg
     4201 tgttggagat gaacatttag aaatactatc taatatagca gtagttttag atgatgactt
     4261 aacagaaaga ctaaaaaatt caaatgacaa acaagctttt atggaagctt ttgccaacta
     4321 atcttaggag ggatttaaaa tgaaaaaggc gattcagttt ggagcaggaa atattggaag
     4381 aggttttatt ggaagcttgt tagttaagtc gggttatcat gtagtatttg ctgatgtaaa
     4441 tgaggagata ttaaactcaa taaacaaaga taaaaagtat actatacaca taagagatgt
     4501 agaatgtata gatgaagtaa ttgataatat aagtgcggtt tcatctataa aagaagaaat
     4561 aatagatgag attgtacagg ctgaaataat aactacagca gttggacctt tagttttgac
     4621 taaaatagcc tctacaattg caaaaggtat aaaagctaga aaagaaaagg gattaacaag
     4681 taatctaaat ataatagcat gtgaaaatgc aatatacgca agttcttctt taaaagaaga
     4741 ggtacttaag tatttaaata aggaagaagt agaatattta gagatgtatg ttggttttcc
     4801 aaactgttca gttgatagaa tagttcctcc tggtaaaaac gaaaatcctc ttgatgtaac
     4861 agttgaaaat ttttatgagt ggaatgttga aaaacaagga tttaaagggg aaataccaac
     4921 tatagttgga atgaatttag ctgataacct tatggcttat atagaacgta aattgtttac
     4981 actgaataca ggtcatgcta taacagctta tataggatat ttaaaaggat ataagactat
     5041 agaagagagt ataaaagata aatttatctg tgatatagtc aaaagtgcaa tggtagaaag
     5101 tggagaagga ttgattaaaa aatacaattt tgactcagaa gtgcactata aatatattga
     5161 taaaatttta aatagattta aaaatccata tcttaatgat gatgtattaa gagtcggaag
     5221 ggaaccatta agaaaactaa gtgataaaga tagattgatt aaacctttaa tgacagcaaa
     5281 atcatatgga ttatcagttg acaatttgat tcttggaata ggtgcagcgc ttcattataa
     5341 taatagtgaa gatactcaaa gtgtagaatt acaagagttg ataaagtcta ttggggtaaa
     5401 aaaagccgtt gctaaaatag ctaatataag caatgacgaa gaaattttaa ataatatcga
     5461 aaaaagttat atatttatga aaaatttata aatttacttg aaaaaaagta gttctgataa
     5521 agtaactata atatattatt ctacacagtt tgaattattt gttgaataat agagaattat
     5581 aatatataat taaaaggaat aaattaatat atttacctgt atatgtttg
//
LOCUS       DAEPOZ010000232         5623 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.232, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000232 DAEPOZ010000000
VERSION     DAEPOZ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5623
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.232"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            310..1146
                     /locus_tag="KR440_003172"
     CDS             310..1146
                     /locus_tag="KR440_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0700180.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFKFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1499..3076
                     /locus_tag="KR440_003173"
     CDS             1499..3076
                     /locus_tag="KR440_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBH0700181.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSDFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            3069..4253
                     /locus_tag="KR440_003174"
     CDS             3069..4253
                     /locus_tag="KR440_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH0700182.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVITNNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIAKR
                     TL"
     gene            4271..4870
                     /locus_tag="KR440_003175"
     CDS             4271..4870
                     /locus_tag="KR440_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBH0700183.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4894..5364
                     /locus_tag="KR440_003176"
     CDS             4894..5364
                     /locus_tag="KR440_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0700184.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEALVSMGEQVK
                     EGTPMLKINRPLIELKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
BASE COUNT         2134 a          575 c         1050 g         1864 t
ORIGIN      
        1 ataaatatat atataattaa gttaaaatac ttgagaatac tggaaaaaac tgaaaataat
       61 ttttgtaata taaaatgtcg attgatttta aataattaaa tatacataag aaataattaa
      121 atatatttca atgaaaatat gataaaatat gacaaattaa ttgatgacaa taaaaaatat
      181 tttgttattg tagtattata gggtaaattg caaaatatat acgaaagtaa aattttaaat
      241 attgacatta aattaaatat actatactat gtaagtgtga tgagagaatt tttttagagg
      301 ggatttatag tgaatataaa aaagatattt aataacaatg tagtagtaag ctccctagaa
      361 gatggcacag aaataattgt cactggtgct ggagtaggat ttaagaaaaa agtaggcgat
      421 ttgatagatg aaaatttgat ttctaagaaa tattttgttc aagacgatca aagggacaaa
      481 tacaatcaaa tacttaataa gacctctatt gaatacttta aaatttcaga agaaattata
      541 gaaaaggcta atgaagtttt aaatactcaa gtgaatgatt caataatatt agccttaact
      601 agtcacatag aatttgctgt ccaaagagaa aaacaaggta taaaattacc aaacttaata
      661 ttgaatgaga caaaacaact ttatagggaa gaatttaaat ttggattatg ggcaattgat
      721 gaaattgaaa aaaggatagg aataaaattg cctgaagatg aagcaggata tattgctatt
      781 cacataatca atggtttgga aaattctcaa aaagatgaag gtattaatat actagagttt
      841 tcaaaacaag ttattcagat aattgaagaa gtacatggat ttaaattgga tgtaaattca
      901 ttaaattata ctagacttat aactcactta aagttttttg ttcaaaggat tctccgaaaa
      961 gaaacttaca atgatagtga tattgaagat atgtataagt tagtgaataa aaattacaat
     1021 aaacctaaag tgtgtactga agaaattgca gcattggtat tagacagatt tgaatataag
     1081 attagtaaag aagaagaatt atatttaatg attcacataa ataaaataac aaacagtggt
     1141 tgttaggatt gtaactgata aagcaggcaa aacctaagtc aatgagctta actattagat
     1201 taagtttttt gatttaggtt tttttattag atacaatcaa aatacagaca gtgactttta
     1261 ggattgtaac tggttaagca ggcaaaacct aaatcattga atttaactat taggttaagt
     1321 tttttgattt aggttttttt attagataca atcaaataca gacagtgact tttaggattg
     1381 taactggttg agcaggcaaa acctaaatca ttaaacttaa ttattaaatt aagttttttg
     1441 atttaggttt ttctataaat ttgattatgt ttttagataa aaataggagg ggaaaattat
     1501 gaaaaaaaat aagttaggtt tatggaactt cttccaaatg ttagggaaaa cgtttatgtt
     1561 cccaatagca ctacttagtg tttctgggat gatgctagga cttggggcag gttttacaga
     1621 ccctaagatg attgaaatgg ttccatttct agggaatgaa tatgttaact tagttttaaa
     1681 cttcatgtta acaataggat tatttgcatt taacaacttg ggagcattat ttgcaatggc
     1741 aattccacta ggattattaa aaaaagaaaa agagtttggt gctttttcag gattagtagg
     1801 atttatagca atgcatatag gtacaaattt ttatttaaca agagctgatt tgttagtacc
     1861 agcagaacag atgtcaacta atggtcaagc tatgattatg ggtatacaaa catataatac
     1921 aagtgtttta ggtggtattg tagcaggatt aattgtttat gcaatatatg ataaagttag
     1981 taatttaaag ataccagaat cattaggatt ctacagtgga ccaagattag ttccaattgt
     2041 tagtttaata gttatgagta tagttggatt attaatacct attgtttggc caccattttt
     2101 caatgcattc caaagtttag gaagatggat ttcttcagca ggaccattag gatatttttc
     2161 atatgcagtg gcagaaagag ttacaattcc atttggacta aatcatcttg taacatctgt
     2221 attccgtttt acaccaatag gtggaactgc tgttattgat ggagaaacgt actttggtac
     2281 attgaacatg tttatggcat atgtagaaaa taatcagatt atacctcttg atttagctgg
     2341 taaaatggaa caaggtaaat tgatgattca atatggatta gctggtgctg cattggctat
     2401 ctaccgtaca gcaaaaccag aaaatagaaa ggcacttaaa ggacttttaa tatcaggagt
     2461 gttgacagtt attataggtg gtataagtga accaatagag tttctattct tattcatatc
     2521 accagcatta tttgctttcc atacatttat gaatggtcta gcaaacatgg tacttccata
     2581 cttaggcgtg ttgatgggat tcacaggaga tttaattgca tttataagtt ttggtgtgtt
     2641 aagaggaaca gctacaggat ggccaatagc agtattagta gcagttttat attttgcaat
     2701 atactatttt gtatttaagt gggcaattat taagtttaat ataaagaccc caggacgtga
     2761 ggataaagaa ataaaagtat ctgaaggaaa tggttcagat ttcaaaaatt tgtctacata
     2821 taaaggacaa caaatgatat ctgctttagg tggtcaatct aatatattat cactagataa
     2881 ttgtgtaact agattgcgtt taaaactaca agatgtatca ttaataaatg aggatgcaat
     2941 taaagaggct ggtggtatag cagttgttaa attggatgag catacaatac aagtaattat
     3001 aggaactcaa gtgtattctt taagaaagca gatggataaa gctatggaag gtcatttgga
     3061 ggcatgtaat gattaatttt gataaaaaag tagaccgatt gggtacatat tgtacccagt
     3121 gggactatgt agaagataga tttggtaaga aggatttgct tccttttaca atttcagata
     3181 tggatttaga gtttccattt gaaataacag aggtgttgca ggaaaggtta aatcatagag
     3241 ttttgggata cagtagatgg aaacatgatg attttagaaa ttcagttacg aattggtata
     3301 ttaaaagatt taattgtagc gttgatagta actggatata ttatagtcca agtgtaatgt
     3361 attcaattag caaacttatt gagatgttaa gtaatgaagg agatggaatc cttataaata
     3421 ctcctgcata caatgctttt tatgatgtga taacaaataa caatcgtaat attataaagt
     3481 cacatcttgt aaataggaat ggattctatt caattgattt taatgatttt gagaaaaagt
     3541 gtaaaaaatc taagatattt attttatgta atcctcacaa tccaactggt agagtatgga
     3601 gtgagttaga acttgttaag atgataggaa tttgtaagaa taatgatgtc aagataatat
     3661 cagatgatat acacatggat attgtatatg acaatagaat gacacctatc ttaaatgttg
     3721 caaaggacta tttagattct gtattcattt gtacatcagc gtctaaatct ttcaatatac
     3781 cagcattgac tgggtcatat gtgattatac caaatgaagg tattagaaat aaatttgaaa
     3841 atatcactag ataccgtgat tttgtaaatt cgccagcaat tctagcaata ttagctacaa
     3901 tggtctgtta tgatgactgc gaatattggt tagatgaact attaaaatac cttaaagaga
     3961 atcttaaata tacgattgat tatataaaag ataatttaga accattgaaa ttggtcatgc
     4021 cagaaggctg ttactttgct tggatagatt tttccgaact taaaataagt ggtgagaaat
     4081 tccaaaaatt attgatggat attggagaag ttgctattat gtcggggtct gtatatggtg
     4141 aagaaggtga gttttattta aggttaaatg tggcatgttc aagagaaaaa cttgaagatg
     4201 gtttaaatag gattaaaaaa actatggatt atattgcaaa aagaacttta tagtaggaag
     4261 ggagatagtt atgggatatt taaaaacaat ggttgatgtt atgactactg aaatgaatgc
     4321 cataagatgt ttaatagatg aagcaggaat agaatatgaa agtatagtta gtgaaattgc
     4381 aaattgtgaa ggcaaagtaa tttttatggg tgtaggaaaa tctgcacata taggtaagaa
     4441 attggcagct acatttgcta gtactggtac acctagcttt tttgtacatg caacagaggc
     4501 agtgcatgga gatttaggaa tgattgaaag taaggatatt acaattttaa tatcaaatag
     4561 tggaaattct atggaagtgg taaactgtat aaaatatatt aaagcaatag gttctaagac
     4621 aattgcattt acttcaaaca gaaattcagt actggcaaaa gagtgtgatt atgctttaat
     4681 atatcctgca aaggatgaag cagaccattt aaatctagca ccaacaacat catctacaat
     4741 tactttggtt ttaggagatt ctattgcatg tgcattatct aagagtagta attttggttc
     4801 ttcagatttt tataagtacc atccaggagg aagtttaggt gagaagttaa aaacagctaa
     4861 caatggataa aagcaaaaag gagagtagtt tttatgttca aaatgtttaa aaaaaaatca
     4921 ataaaatcac ccatatcagg aaaagtaata gagttatcta aaatacaaga tggtgtattt
     4981 tctcaaaaaa ttatgggaga gggtgtagct atagattcta caggagacat tgtatatgct
     5041 ccaatcaatg gaaagatagt agtagtagca gagactaaac atgcatttgt aatagaatta
     5101 gaaaatggga tggaacttct tattcatgtt ggtttagata ctgtaaattt aaaaggcgaa
     5161 ggttttgagg cattggtgag tatgggagaa caagtgaagg aaggaacacc aatgttgaaa
     5221 attaaccgtc ctttgattga attaaagggt atatcactta ttactccagt tacaataact
     5281 aatcattcag agtacaatat gaatacatgt aatgtaggca atagcgttga aggtgggaaa
     5341 gatactgtaa ttgagtttaa ataaagaatt aaaatacaag gttaatattt ataaatacct
     5401 ataaacttat gttttataag attgattgta tggatttatt gtataaaaaa gaactcttta
     5461 attataaatt aaggagtttt tttattttgt aaatattgtt tagatttata tagaaaaaca
     5521 cttagtaata aatagaataa ataagtattt atatagctaa aaatctaaat agttttgttt
     5581 aaatatattt ttatattttg taatttcaaa ttaataaata att
//
LOCUS       DAEPOZ010000233         5590 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.233, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000233 DAEPOZ010000000
VERSION     DAEPOZ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.233"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            350..1198
                     /locus_tag="KR440_003177"
     CDS             350..1198
                     /locus_tag="KR440_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBH0700185.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGTEEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            1277..3199
                     /locus_tag="KR440_003178"
     CDS             1277..3199
                     /locus_tag="KR440_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBH0700186.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDIHDSSVIAAYLEGCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            3281..4144
                     /gene="aroE"
                     /locus_tag="KR440_003179"
     CDS             3281..4144
                     /gene="aroE"
                     /locus_tag="KR440_003179"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBH0700187.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGIEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNAKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIESSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            4344..5561
                     /locus_tag="KR440_003180"
     CDS             4344..5561
                     /locus_tag="KR440_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0700188.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAILIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLIVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
BASE COUNT         2059 a          680 c         1067 g         1784 t
ORIGIN      
        1 tttttacatt tttattatat atgattatag agtaagaata aaagctttta aaaaaattta
       61 tcgttttttg tctgaattat aagataaata tatttataca acctaataga aaaatacaag
      121 aattatctta taaataaaat ctataaacaa aatctatgaa ttaataggaa attatcaatt
      181 tgttattagt aaagtctcgt gatacaataa atttataaaa tctagataaa tagaaatcaa
      241 aaaatacttt tatttttaag gtaaaataat actactagta ggataaataa aaatataaaa
      301 tcaaagatac tattaattat aaagaaaata aagggggcaa atttaagcta tgaataatca
      361 aaaaaatctt aatattccaa ttacaattag ttcttataca ttgggaacag aagtttcatt
      421 ccgtgataga gttcgtattg caaaagaagc tggatttgat ggtattggat tgcgtgctga
      481 aaattatatt gatgcaaaaa atgcaggtgt tacagatgaa gaaatgattg caattcttaa
      541 tgaatatgat atgaaagtta cagaagtaga atatataact caatggggaa cagaagaaga
      601 tagaactgaa gctcaaagag aaaaagagca aaatgtttat catatggctc acttatttaa
      661 tgttaaacat ataaattgtg gattattaga gatgatacca gaagaacaaa ttataactgc
      721 attgggagaa ttatgtgaca gagcaggtga acttattata ggtcttgagt ttatgccata
      781 tagtggtgta ccagatttgg ctactgcatg gcgtgttgtg aaaggatgta atcgtgaaaa
      841 tgcaatgctt atattagata cttggcactg ggctagagca aagcagacag cagaggacct
      901 tgcacctgtt ccagcagata agatagtatc aattcaagtg tgtgatgttt tagaaactcc
      961 ttatgaaaaa cttagggatg aatcactaca tgaccgtttg gctccaggtg aaggatatgg
     1021 aaacacagag gaatttgtga aaataataaa agaacacaat atatcaccaa gagtcatagg
     1081 ggcagaagta atatctgatt ctctaatatc taaagggtta tccactgctg ctgaaactgt
     1141 atttaatgca actaaaaaag ttttggataa agcatggtct gaagtatctc caaaataaac
     1201 ttatataata taaaaatata cagagtatta taatataaaa cttttaaata taaaacttga
     1261 aaggaaggaa aaccttatga agattagttc tatgtttaca cctataagaa ttggttcaat
     1321 gacagttcca aatcgttttg tagttccccc tatgggaaac aattttgcaa acacagacgg
     1381 tactttaagt gaaacttcta aggcttatta tcttgagcgt gctttaggtg gttttggatt
     1441 aataactata gaatctagtg ttgtagacaa aaaagcaaaa ggaggtcctc gtaagccttg
     1501 tttatatgat gatagcacaa tagatagttt taaaaatgta atagatgctt gtcatgatgc
     1561 aggttcgaag gtatctatac aattacaaca tgctggttct gaaggaaatg aaaaagtagc
     1621 aggacatcca ttaaaagctg cttctgcaat acctgcatca aatggtcgta atacaccact
     1681 agcaataaca acagaagaaa tatatgaact tatagaatct tatggagatg ctgcattacg
     1741 tgcacaaaaa gctggtgcag atgctgttga agttcactgc gctcatggat atctggttag
     1801 tagttttata tcacaaagaa caaataagcg tgtagatgag tttggtggat gttttgaaaa
     1861 tagaatgaga ttacctcgtc ttatcattga aaatatacgt aaaaaagtag gaaattctct
     1921 agcaatatta tgtcgtataa acagcactga tgatgttcca ggaggaattg atatacatga
     1981 tagttcagta atagcagcat atctagaagg ttgtggaata gatggacttc atgtttcacg
     2041 tagtatacat atacatgatg aatatatgtg ggctcctacg actttacatg ctggattcag
     2101 tgcagaatta gttactgaaa taaaaaaagc tgttagtata ccagtaataa cagttggacg
     2161 ttatacagag ccacagtttg ctgagttaat ggtcagacaa ggaagatgtg atttagtagc
     2221 attcggtaga caaagcttgg ctgacccaga aatgcctaat aaagctaaaa atggaaaact
     2281 agacgaaatg ataccatgta ttgcatgctt acaaggatgt gtaccaaata tgttccaagg
     2341 aaaacctata gcctgtcttg caaatcctat attaggacat gaagcagagc ttaagccagc
     2401 tgaaattagc aaagaagttc ttgttgttgg tggaggtgtt ggaggtatgc ttgcagcatg
     2461 ggtatgtgct aaaagaggac ataatgtaac tttagttgaa aaatctgaag ttcttggtgg
     2521 gcaaatgcgt ttagctgcat atcctccagg gaaaggagat attactaact tagttagaag
     2581 ttatatttca aaatgtaatc aatatggtgt aaaaatatgt actaatacag aagctacagt
     2641 agaacttata aaggagaaat cacctgatgt agttattata gctactggag caactccact
     2701 tgtacttcct ataccaggaa tcaatgattc aggattaatt catgctgtag accttcttga
     2761 tggtaaaaaa tcttgtggga aaaaagtact tgttgttgga ggtggaatgg ttggatgtga
     2821 agtggcagcc ttccttggag aacaagaaca tgaggttact gttattgagc ttagagaaga
     2881 agttggtgca gatgtaattt cagaacaccg taaattcctt atgaatgatt ttgaaaatta
     2941 taatatacat accattactg gtgcaaaagt ttctaaattt tatgatgatg gtgtcgacta
     3001 tttacttaca gatggcacag agcatgattt aaaaggtttt gattcagtag tacttgctat
     3061 gggttctcgt aattacgacc cattaagtga agttataaaa gaagttgtaa aagaaactta
     3121 tattgttggt gatgcagtaa aagctcgtag ggcattagat gcaacaaaag aagcttttga
     3181 agtagcatta aatatataaa taataaataa atttttcttt gtaaataact gaataggtta
     3241 tttgcaaaga aaatgataat attgggagga aattatagtt atggaaattt caggtagaac
     3301 aggattattt gcattaatag gtacaccagt tggtcactct aaatcaccag tgatgtataa
     3361 ttatagtttt aagaaattag atttagatta tagatattta gcctttgata tcacagtaga
     3421 taaggtaaaa gaggcacttt tagcaataaa aacttttaat ataaaaggag ccaatgttac
     3481 tatgccatgt aaatctgctg ttacagaata tatggatgaa ctttctccag cagctcgtat
     3541 aattggagca tgtaatacaa ttgtaaatga caatggaaag ttagttggtc atataacaga
     3601 tggagtaggc tatgttcgta atttaaaaga aaatgggata gaagttaaag gtaaaaagat
     3661 tacaattatg ggtgctggtg gtgcagcaac tgctatacaa gtacaatgtg ctttagatgg
     3721 agcaagagaa atatctatct ttaatgctaa ggatgatttt tataaaagag cagagcagac
     3781 agttgagaat ataaaaaaag atgtaccaga atgtgttgtt aatctatatg atttagaaga
     3841 tacaaacaaa ctttatgagg agatagaatc aagtgatata ttgacaaatg caactcttat
     3901 tggtatgaag ccttatgaca atgaaacaaa tataaaagat actagtgttt taagaaaaga
     3961 cttagtagta acagatgtcg tttataatcc taagaaaaca aaaatgattg aagatgctga
     4021 agctaatgga tgtaaggcta ttggtggatt aggaatgtta ttataccaag gagcagaagc
     4081 ttttaatcta tatactggat tagaaatgcc tgtagaagaa gttaatgaat tatgctttaa
     4141 atagataaga gtgatatctt aaacaatgaa aaaacatacg tatttcaata tttttaataa
     4201 taaattacaa attgtcatag tgggttttgc atcatagtcc agttaaattt gaagctttaa
     4261 ctgactatac tttaaatcca ttatgaggat gagtttattg tacacaaaaa tgttaataaa
     4321 ttaacaagaa tgggagaaaa attatgaaca ataaaaaata ttatcctact gccatagcgt
     4381 tgtatttttc atatttttta cttggtattg gaatttcaat tttaggacaa tacaaaccag
     4441 aattttcatc aatgtggggg gctaaaacac tatcagatgg aacattggat gtaagtattg
     4501 ttttagctgt tattgctgca ctaggattag ggagacttat atcttatcca tttgcaggac
     4561 ctatttcaga taagtatgga agaaaagtaa gtggtcttat aggaaacttt ctacatgcaa
     4621 ttttctttgt tggaattgta ttttcaccaa atttttatat agcatatgta tttgcaataa
     4681 ttggaggggc agcaaattca ttcttagata cctgtgtaac tccttcttgt atggagatat
     4741 ttgctagttt aggaacaata gcaaatatgt ttactaaatt tacaatagct ttagcacaat
     4801 ttttgcttcc ttttataatt ggatttgttg cagcaaattc aatatcattt aaagttattt
     4861 ttatagtaac agcgattctt attgtagtag atgccatttt gatagctgtg ttaccatttc
     4921 caccagctaa taatgttatt gataacaaag gcaaaacagt taaatcagag aagatgaaat
     4981 ttacaccaac aagtatagct cttgtttgta ttggatttac atgtacatct acatttgtat
     5041 tatggttaaa ttgtaatcag gaattgggta aattgtatgg gatggctgac cctacaaaaa
     5101 ttcaatcctt ttactcaatg ggagtaatat gtgcagtatt aataacttct ttacttatta
     5161 aaaaatatat taaaccaata agaatattag ttatatatcc tataatagct ttacttatgc
     5221 tattaattgt atattttgtt caaactccaa ctatttgtat gttaggggga tttgtaatag
     5281 gttattcagc agcaggaggt gttcttcagt taacaacgtc aactgcaaat gaaatgtttc
     5341 caactaataa aggaaaaata acatctatag ttatgattgc atctagtata gcaaactatg
     5401 ttattttaaa tatagcaggt attattacaa aatcaggggg agttaatgga ccaaaatatg
     5461 ttgtattgtt taatgtggca ataacatttg ttggaatttt acttgcattg tttgtaaaca
     5521 tgagatatga aaaagaaaaa gtgtatgatt atgatgtcta atattagtat aatatatgca
     5581 tagagaagtc
//
LOCUS       DAEPOZ010000234         5544 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.234, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000234 DAEPOZ010000000
VERSION     DAEPOZ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5544
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.234"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(119..280)
                     /locus_tag="KR440_003181"
     CDS             complement(119..280)
                     /locus_tag="KR440_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003489153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0700189.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFD"
     gene            complement(454..1320)
                     /locus_tag="KR440_003182"
     CDS             complement(454..1320)
                     /locus_tag="KR440_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH0700190.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNVEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTREYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            complement(1546..2415)
                     /locus_tag="KR440_003183"
     CDS             complement(1546..2415)
                     /locus_tag="KR440_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBH0700191.1"
                     /translation="MDYKTIILNRKSVREYKNIDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNKSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(2921..3142)
                     /locus_tag="KR440_003184"
     CDS             complement(2921..3142)
                     /locus_tag="KR440_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700192.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            complement(3643..4623)
                     /locus_tag="KR440_003185"
     CDS             complement(3643..4623)
                     /locus_tag="KR440_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700193.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRVYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(4787..>5544)
                     /locus_tag="KR440_003186"
     CDS             complement(4787..>5544)
                     /locus_tag="KR440_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBH0700194.1"
                     /translation="KEFDNFKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDL
                     VDGENKNFQVALDLIDNLVEKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIIN
                     MENESVRIYRGNSHLNLYGLIIPLECYPYLFANNKKLELSDNFVEDNLGKVNENEYNI
                     LLAHTPFFFEKYAKWGADLVLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDN
                     STMILNKGLGGSKVLIRLNCKPEIVKITLKYEK"
BASE COUNT         1854 a          938 c          522 g         2230 t
ORIGIN      
        1 tagcaattca agtagtattg gataggttct atcctatctc tacatataag tttccctata
       61 tgctgactat tttagctact aactcatgac taaagtcaca agtgtgcgta gctatttttt
      121 aatcaaagta tctagctatc ttaaaggcta agatgataga aggattatac ctaccatttt
      181 ctaatgaacc tatagtttgt cttgatactt gcaaaataga agcaagttct tcttgtttta
      241 tgcctctttc ttttcttatt tcttctaatc tatttttcac atctatcccc ctttaatatc
      301 tcaactacgg aaagtctact ttccataatt atacaactat atttcattta tgtaaagctt
      361 gctttccata tttacaaaaa aataacagac tattttcagc ctgtaatttt aaaatttcat
      421 gttgtttatt aaatttaatg ttattcatta actttaactt ggtttctgaa ttggaatcca
      481 aacttctgta gtataatcag gagcattgat atcgccattg ggataaagtt caatatctgg
      541 taagttagca tacacatacc cagaacttgg taaccactca gtaataattc ttttttgcaa
      601 ttcctgtatt gccagagcat ttggcattgg agaaacacat tcaaatactg cccatgtacc
      661 tgatggtatt aagtattcac gagtatcttc taatacaggc ttatctgttg gtgcagcaat
      721 ataataatct aaacccttac tatttgtaca cacactaact ccaagcaatc cacaaggttc
      781 gttatttatt aactctgaaa gtttatcaat tgttccattt tttatgcatt tagcccataa
      841 cttaggaact ttctcaaagc actcttctac aatcatgttc atactttctt ttataccaac
      901 aatacgaaac cctttctttt ctacaatacg atattgcatt tctacatttc cttttacata
      961 aatgcttaat gtcatcttag gatatgcttt taaaacaaca ccctttgctc tagctgcaga
     1021 tggagaaatg ttatgtatat tttgaaaagc tctattaaaa gatgttggtg aatcgtaacc
     1081 atacttctca gatagttcta aaattttgat gttactgttc tgaagttcaa atgcagcctt
     1141 agtcatacgt cttcgtctaa tatattcaga taatggtact tctgcaatat atgaaaacat
     1201 tctttgaaaa tgaaaggttg aacaacatgc aatttttgct gcttctgcat aatccactcc
     1261 actttcgaga ttttcttcaa tataagtaat agcttgattt aatttattta tccattccat
     1321 ttgatattac ctccaagcaa attatacagt ttaataaaat aatatacctc tctattcatg
     1381 cacaaaattg taagcattta aattcataag tatttttcaa tcaatacaat aaatataaaa
     1441 attgtattat acaataaaaa agttcaataa cattttatta taaaatatta ttgaactttt
     1501 tataaaaatt aatttaagtg tagttaaatt aagtattaaa ctatcttata tattacaata
     1561 tcctactatc ttatagtctt ctggcacttt ataatctttg tctggagtac ctaaattcca
     1621 ttttaaatca aacatagttg cacttattat tgttgcaaaa taaagcataa ctataccaat
     1681 gtctattttt tcttcatata aattagtatg accatctttt ctaacagcta aaactacagt
     1741 gcctccatca actataaatc tccatggttg tctatttaat gtagatggag ctaatcttgc
     1801 atagtgaaat gcatcaagaa gtgctctttc ttctaagatt tctatacttg caacatttcc
     1861 ccattcgttc atataaacaa catcttctac acctagtcta gtagacccct tatctgttac
     1921 aacttgcata tcagattttg aataattttg cccagttttt gtagcattta atactttagc
     1981 attacttaca ttttctccat atccaatagc tataattcct gttacctctt tatctgaatt
     2041 aattccaagt ttttctttta ccttttttcc atcttcaaaa gtaacccaac aagaaccaat
     2101 tccaagttct gttactttaa agattatact ttctcctatg taccctgaat tttcaatgta
     2161 attatctttt atgtcagata atattataat ataacttgga gaatctatca tatttccttt
     2221 gtaccctgct actttatcta aattattaaa aacttcactt ttattcataa ttcttacatc
     2281 aacatctatt tctggaacta gtttcttaca agattctgag tatttcttaa tttcactaag
     2341 atattcttct ttaatatcta tgttcttata ttctctaaca gactttctat ttaatatgat
     2401 tgttttataa tccataattt tatcctcccc aaactagtat tgtcttaatt atatacccat
     2461 taatatatta ttttactcat ttatttaata aataaagatt acaattttgt aattattatt
     2521 tattaataat attccacttt tacattaata atgaccagtt gtgcaaaatc cattgccaac
     2581 caccaaataa aaacatatta taaaagtaat ataaaataac aaacaaatct taacaaaaaa
     2641 ttaaactttt tttacacgaa agaaggcttc ccgttgcctt ctttattttt ataaaaaaat
     2701 aagctgtttc taaaatgatt tcacattttt gaaacagcca agttattcta taatgacaac
     2761 tataatttga ggatgtgagg gagagttgtc attataaatt tagggtaatt atattatgtt
     2821 ttaagctagt tcttcctata tttatataat acattttata tatgaatttt atatgagatt
     2881 ggtataaatg atatatgaaa ttacatatgt ttatattata ttaaatttta ttattacatt
     2941 ttccacaaca atactcatat gtctccacat tatttggaat atttacaaat acaaattctt
     3001 tacagttctc acaatacttt aatatagata tagttccaca agtgtaacat tcaaatatta
     3061 cacaatcctc attttcctct actatttcaa actttttact atgttcacca cactctgggc
     3121 acttgtgatt atttacattc ataaatactc ctccttatta gtatagttac atttcaaaaa
     3181 cttaattaat gtattctata ttacttatta tatattctat aataaatcat attctaatat
     3241 aataaaacaa attaaataaa aaaaaaccat aaattttaaa tttatggatt aattcttgta
     3301 atgataactc tatttgagga tgtgagggag ggttatcatt acaatagggt attatattat
     3361 ggtttttaag ctagtctttc tacacctcat atgatacata tgctatatga attttatatg
     3421 atttttacat aaatatttta tgaaatttaa attttttagt cattttgaaa tttgtaaaaa
     3481 gttctctaaa aatccacagc atcataaaca gccaaaattt aagtaaaaca tcataaaaaa
     3541 atttgtccaa taattttaac aagtcatttc acctcattat aattattgac acaaacttaa
     3601 ataaacaatc tataaatctt atataaatct taaactttac ccttattgat acacaatttc
     3661 tagtttgtca attttatttt gcatctctaa gaaaatttct tcaacagaat tgttaaatat
     3721 attgcctaaa tagactcttt tatctttttg aaatgaataa gattttacac taccatcaga
     3781 agaaatatat aaatgactcc actctgctcc atattttgaa aacctctcct ttgtatcttt
     3841 aaacctcata tcttctattt ttattgcttt ttctctgtct cctcttacta cagtgtgacc
     3901 cctttttatt aaactggtta tttttatatc ttcttttaat ttaatacgtg tatcatcatt
     3961 ttttgaatat actttccata tcactttatg acgataatta tctaattctt ttattaagct
     4021 tctatcaata gtaaagctat ctttagttac cacaattaaa tcaaaatggt ctaacatata
     4081 ttgaaatatt tctctcatac ttttattttt taaaatatcc tcaccaataa cctctataaa
     4141 tctacagcct ttctcataca tgcagttcaa taaacttgtg atttcagaaa aataatcatt
     4201 tgtattatta tctccatcta tttctatagt aaagtacaaa ggagtaatgt atgatttatt
     4261 tcctgttatc tcaaatacac ttgatagtgt ctgtatatca tcttcaaacg ttaagtatac
     4321 attctcataa aacataaaaa attccataac tttttctttc atattatgga tatttctata
     4381 tctattggat aatatattta ttatatcgtc agtagtattt ttcccatcac attcagccag
     4441 tataacaatt tgcttccaac ttaagctatc cacatttccg atactttttt tatatccatc
     4501 ttcttcatca taaataggac caattataat gttttctttt ggccacagat gaaccctagt
     4561 tccatctttt aatttcggat atttattttt tacagactca taaaatctac ttgtatcacc
     4621 catgattctt tactcccccc catagtttaa atattctatg ctaaaaatag cactttattt
     4681 ttattaattc aacaattatt caaaattggt cattatatat gtttaaatgg atttaacatc
     4741 aaccttattt atatgttatt gatgtttata tatgttatat aaaatcttat ttctcatact
     4801 tcaatgtaat cttaactatc tctggtttac aattaagcct aattaaaact tttgagcctc
     4861 ctaagccttt atttaaaatc atagtgctat tatctttttc atattttccc cagtcatact
     4921 taggaaagaa ctctctattt ggagataata cacctccaac aaaaggtatt cttattatac
     4981 ctccatgaac atgtcctgct aaaaccaaat ctgctcccca ttttgcatat ttttcaaaga
     5041 aaaatggtgt atgtgctaaa agaatgttat attcattttc attaacttta cctagattgt
     5101 cttctacaaa attgtcactt agttctaatt ttttgttatt agcaaataaa tatggatagc
     5161 attccaatgg tataattaaa ccatataaat taagatggct attacctcta tatattctta
     5221 cagattcatt ttccatattt attattttct ttgaatatag ttccttaaaa taatccttat
     5281 atagatgttt atactttttt attaaagatt tttgttcatg atttccaatt atatgatata
     5341 cttcatattt ttcaaccaaa ttatctatta aatctaatgc aacttgaaaa tttttatttt
     5401 ctccatctac taaatctcct gttataaata taacatctgg gtttaatgat tcaattttat
     5461 ctatcaatac cttattattt ttatcaaaca ccttattatg taaatcactc acttgaacta
     5521 ttttaaagtt atcaaattcc tttg
//
LOCUS       DAEPOZ010000235         5526 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.235, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000235 DAEPOZ010000000
VERSION     DAEPOZ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.235"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            87..3035
                     /locus_tag="KR440_003187"
     CDS             87..3035
                     /locus_tag="KR440_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0700195.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPLEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            3013..3477
                     /locus_tag="KR440_003188"
     CDS             3013..3477
                     /locus_tag="KR440_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0700196.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            3590..3892
                     /locus_tag="KR440_003189"
     CDS             3590..3892
                     /locus_tag="KR440_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBH0700197.1"
                     /translation="MGVTNVTKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            3954..5318
                     /locus_tag="KR440_003190"
     CDS             3954..5318
                     /locus_tag="KR440_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBH0700198.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKL
                     SIEE"
BASE COUNT         2238 a          600 c         1001 g         1687 t
ORIGIN      
        1 tactttataa aaaaataata agtacaaaaa aaacaatata tggcatataa gttgctatat
       61 aaataataag gagggaacaa ttataaatga ggcaaagaat aaaaaactta attgaaaatg
      121 aagataagaa aaatccttta acagatgaag cgatttcaag taggttaaat attcgaagag
      181 aagatgttac ctttttgaga aatgagttaa aaatagaaga ttcaagacaa agaagaaaag
      241 ttgttttaat taaagctata aaagatattt taaaagaaga aaaaactata aataaaaatg
      301 atataacaag aagattaaac agcattgggt ttacagtatc aagatttaca gtcatacaat
      361 acttaaaaga gattcaagat tctggtgacc taaaacttga taaagagagt gatgataaaa
      421 aagaagaaat aatcaataat agagaaaata aagatatagc atttgataaa ctcataggta
      481 gtagtggtag cttaagtaca attataaaac tagccaaagc agcaattctt tatccacctc
      541 atggtcttca tactattatt atggggccaa ctggtgttgg aaagagtgag ttagcagagt
      601 gtatgtataa atttgcagta gaatcatcca gattccctaa gaatgcacca tttatagttt
      661 ttaatgcagc agattattca gagaatccac agcttttgct atcacagtta tttggacacg
      721 ttaaaggtgc ttttacagga gctgatgaga acaaagaagg attagtatca aaggcagatg
      781 gaggaatatt gtttatagat gaaatacata gattacctca tgaaggtcaa gaaatactat
      841 ttcaactgat tgataaaggt atggtcagaa agttaggaga aacaagacta acacataaaa
      901 tagatgtaat gattatttca gcaacttctg agccaataga ttctcattta cttaatacat
      961 ttaaaagacg tatacctgta acaattgaaa taccagaatt agttgcaaga ccattaaatg
     1021 agaggtttga cattataaat aatttcttct taatagaagc tcaaagaatg ggagcaaaca
     1081 tacatataaa gtcagatgta ttaaaagctc ttatgcttta cgattgtata ggtaatgtag
     1141 gtcaacttag aagtgatatt caagtagctt gtgctagggg actattaaac caacttacaa
     1201 acaaactaaa agaagtaaat ataagcatat cagatgtacc tggatatgta aaacaaggtc
     1261 ttataaaaat cagaaattgt agaggtaaaa tagaaaatta tgtagatggg gatttaatta
     1321 tagattcaaa tcttcaaggt gagatagaaa agaaaaatga agatatatat acttttccag
     1381 atgaaatata caaacttact gaaagaagac atattgattt attggaacaa ggtttagatt
     1441 ttgatgttat aaatagaatc attggaggag aattagaaag ttgcattcaa aaatacataa
     1501 aacaagttaa aaaagtttct attagtaaca atcttccaga tataagtcca attgttggaa
     1561 aatatatcgt agagctaact tatgaagtaa tcaaaatagc atcacaatac ctaagttcac
     1621 tagaaccgaa tctacattta tgtttagcag ttcacctaaa agcagctttt gagagattaa
     1681 aagaaggaaa agtaatatca aatgctcatt tgaaagaaat aataggaaat tatccactag
     1741 aatattctgt agcagaaaag gtagctcaat atttaaagga aagatataat ataaacttac
     1801 caaaagaaga aataggtata atagcaatat attttaaaat gagtagtaga agagatatgt
     1861 ctgaacaaaa aaagataggt gtacttctta tggctcacgg aagagtagca tcttctgtat
     1921 gtgaggttac aaataaacta ttaggaatta atcacgcaag gtatttagat atgccatttg
     1981 agaagaagcc ggaagaaatg attgaagaag ctactacaat aataaaagat attgatgaag
     2041 gtagaggtgt gattatatta gtagatatgg gttcactttc atattttgga gatattattt
     2101 ctggaaagac tggaattaat ataaggacaa tatctagatt tgatacattg ctttctatag
     2161 aggttatacg aagagcagtt ttaccagatt taacaataga cgagatagtt gatgaagtta
     2221 aaggtataga gccacaagta aaagaaaagg ttagtaaatg taacaataca aaagtagatt
     2281 ttagaaaacc tataatttta acattatgta ttactggatt agggggagct tgtaagataa
     2341 aaaaaatcct agaggaaaat attaaaagta taactgaaaa ggttgatata gaacctatag
     2401 gtatgattga atatccaagt cttgatttag agataaaaca aataagcaat gataaaaatg
     2461 taatcgccat agttggaaca attgaccctc agatagcaaa tatccctttt tttagtctag
     2521 tagacataaa tgataaagca aagttaaatc aaataaataa cctaattgat acaaatcaaa
     2581 ttggtgaaac aaataaaaaa tatgaattat cagatttaat acataaagaa gatatatttg
     2641 tggatttaca ggttaaatca aaagaagaag ttctaaaata tatgagtaaa aaacttagag
     2701 agaaaggtta tgtgacaaaa aatttctata aaaaagtagt ggaaagagat aacttatttc
     2761 caactgaatt atcagatgaa atagcaatac ctcacacaga tggaatagat gtaattaggc
     2821 cagctataag tatagccatc ttaaagaagc ctattgtgtg gagtaaaagt aaagtaaaaa
     2881 taatactttt attagctgta gaccaaaagt gttttaatcc attaagcact ctactctcat
     2941 ttatagaaac agatgaattt aagaagataa aaaacattaa agatagagat tttattaggg
     3001 agatgatatt agatggagtc aatgaaaata attagtagca atttaatttt taaaaatatt
     3061 gaagtgtcta acaatgaaga tgcattaaag tttttgggac aacgtttgtt tgacgaacag
     3121 tatgtaaaag aaagctacat acaagctgtt gtagcaagag aaaaaaagta tgctacagga
     3181 ttaccaacag aaatatacgg agtggcaata ccacatacag atatagtaca tgtaaatgag
     3241 ccaggaatag caattggaat actgaataaa ccagtaaaat ttataatgat gggtacagac
     3301 gatactgaaa ttgatgtaaa agttgtattt atgttagcag tgaaagagcc acaagaacaa
     3361 ttgcaactat tagaaaaact tatgactata tttcaagata agaatatact aaataatatt
     3421 gttgacttaa gtgaagaaag tgtgagtgat ttattaaact ctaaattgaa aaattaaaca
     3481 tgtaaagtat attaaaaatg taaaattaaa catgcaaagt atgttaaaaa atataaaaag
     3541 attattaaag atttctaact atagatatct ttaaaataaa ataatttcaa tgggggtgac
     3601 taatgtgact aaaaagatat tagttgcatg tggaacagga gtatgtactt caacaattgc
     3661 tataaacaaa ttaaagaaag cacttcaaga cattggtaaa ttggatatgg ttaatattac
     3721 acaatgtaaa gtagtagaag ttgcatcaaa agcacctgat tatgatttaa taatttgtac
     3781 tacacaagta tctagtagta taaaaacacc agtgataaat ggattacctt ttttaactgg
     3841 tgtcggaatg gataaattaa tcaatgatgt attagaagag ttagaattat aattaaaaaa
     3901 attaaggaac atggtctcta aaatcataag ttttagagac ttaaaattaa ataatggagg
     3961 gtctttatat ggagatatta aatttcattg ttggtctagg tgcacaagta atgatgccaa
     4021 taataatatg tatttttggt ttgatattag gtacaaaact aggaaaatct ttaagagctg
     4081 gtttaactgt gggagttggg tttataggtc ttaacactat tatagctttg ctaacagata
     4141 atttaggtcc agctacacaa caaatggtta agaatttagg gctaagttta tcaataatag
     4201 atgtaggatg gccagcagca tcagctatag cttttgcatc aacagtaggg gctttaatta
     4261 ttccaatagg tcttgtggta aatatagtta tgctaattac taatactact caaactgttg
     4321 atgtagacat atgggattac tggcattttg cttttacagg agctttagtg gcaggggcta
     4381 ctcaaagtgt aatgtgggga gtatttgcag cagtagcaaa tatggtaatt gtattggtaa
     4441 tggcagattt aactgctcca ggtatagagg aatatttagg tatgccaggt atatctcttc
     4501 ctcatggatt tacacaggca tttgttccaa ttgctatagt agtaaataaa ttattagacc
     4561 taataccagg tataaataag attgagataa atgctgatac attgcagaaa aaatttggat
     4621 tatttggaga acctttaata atggggtctg taataggtgt aataattggt attgctgcga
     4681 aatatgatat aaaaggaata ttacaattag gtgtaactat gggagctgta ttaatattaa
     4741 ttccaaagat ggcagcatta ttaatggaag gattattacc agtatcagaa gctgcacagg
     4801 aatttattga gaagagattt aaaaacagag gcaagattta tataggctta gattcagcag
     4861 taggaatagg tcatcctgtt acattatcag ttgcactagt gttggttcct ttgactatac
     4921 ttatagcagc tatattgcca ggaaataaag ttttaccatt tgcagattta gcagtaattc
     4981 catttgcact tgtattaatc gtaccaatta caaagggaaa tgtgtttaga acattaataa
     5041 taggaataat tataattact tctggattat taatcgcaac taacttagct ccattattta
     5101 cccaaatggc tttaaatgct agctttaaaa tgcctgaagg agcaactatg atttcaagta
     5161 tttgtgatgg tgcgaatcca ttaagttggg tatttgtaaa agttatgaac tataaagtta
     5221 ttggaggagt agtttttgga gttatagctt taggaatggc tatttacaat aggaatagaa
     5281 taataaatga aaataaaaag ctatctatag aagaataagg ttaaaacaat tttattaagg
     5341 agtgtatagg ttatgaaaaa atataaatat gattctaaaa ggtacttact cagtgttaca
     5401 ataccaggat tttttatgat tttaatattg atttatgcac tgtttaataa ctatataaat
     5461 tttaatttga atatatatag tttgttaata attgtctgta cttatggtat attcaatact
     5521 tttata
//
LOCUS       DAEPOZ010000236         5446 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.236, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000236 DAEPOZ010000000
VERSION     DAEPOZ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5446
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.236"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            152..2017
                     /locus_tag="KR440_003191"
     CDS             152..2017
                     /locus_tag="KR440_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HBH0700199.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEDSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIEYNKIEFEKEINDILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(2195..3559)
                     /locus_tag="KR440_003192"
     CDS             complement(2195..3559)
                     /locus_tag="KR440_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0700200.1"
                     /translation="MSQLLIKNAYLITMNADREVYETGSILIENNIIKNVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLIRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHNPGAAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(3706..5049)
                     /locus_tag="KR440_003193"
     CDS             complement(3706..5049)
                     /locus_tag="KR440_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBH0700201.1"
                     /translation="MVPNTNNVQPDGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     regulatory      complement(5122..5223)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
BASE COUNT         2044 a          699 c          803 g         1900 t
ORIGIN      
        1 acagttataa gatagggtat ttacataatt aataataagg atattactag aatttcaatt
       61 tctagtaata tttttttgcg tataaatgta aacttgacga aataagtagt ataatcaata
      121 taaattacat catgtaaagg aggataattt ggtggagaat gtttttaagc gattgcagga
      181 atttaatggt tatgatggat acaaagaatc atttgaaatg aattacctat gtatatatga
      241 aagtataccg ttaagagaac aagtagaact tgcaaataat ctagtagatg aaattttaaa
      301 tatgtataaa agtgaatcta atgaaattta tttattggag gatagtaatt ctaagtcttt
      361 aatttgttat ttcgaaattt ttatgaaaaa gataaatact ttggtaaaag aaatgattat
      421 tgatgagaaa tggttatata aattaactaa agaattaata tataagagta aaaaagttga
      481 gtatgtaaaa ctagggttag ttttaagtga gaaatatcta aatgtagaaa acttaagaga
      541 agttgtagat acattttcta aatctggaga atacgtattt tatctgagta atactattaa
      601 aaaacttgaa ttttacaata catatttatt taatttatct aaaaaggcta caggttcaat
      661 aaaagtattt gctattgtta atatggaaaa tttagattct aaaataaatt cctatctaat
      721 tgaagatggt tataaggata caaagtacga acgattattg atgaactata ttatttcaat
      781 tgtagatttg aatgaatatc ttgaaaaacg agatttagat aaagaaaaga taaataattt
      841 agcacgtctt atatgcaatt acttgctaag tgtagaattt aaatatatag gtaataaatt
      901 agaattagtg aatcgatttt tacctactgt agtaaattat gggactaact ttgaaagttt
      961 atattcaatt ttcctaatag ctataaacgt cttaaaagat gaaaatatag agtacaataa
     1021 gattgaattt gaaaaagaaa taaatgatat cttgctcagt gaaaaatgga aaaatatata
     1081 ttttgaagca ttaagagatg ctagtggaaa aacagaagat attataaaaa tgtcagaaat
     1141 ttatgatgta aacttatctt ttgatgactt gttgccatat ctgaatagag atattagaga
     1201 ttttgaggta tattggcata tatctaaaaa gggaacgaca agttctagat taaaattatt
     1261 aaattttttt gaagagacgt ttaaaataga tgatttaatt ggaaaaatga aagatataga
     1321 aaaagataaa ttgactcaag aatattatga tgatatgtta ttttttattg tgcttaaagg
     1381 aagtaaatca ctatatcctg aaggaaagaa tatatcttta aaaggaatat ttggaaatat
     1441 aaatgaagta agaaaagaat ccataaatat cttaaaaaga tacagagaga aactatctct
     1501 tgaagaatta aaaattgtta aagaagcgta tgaaaaagaa aaaaatgtta tattgaagga
     1561 tgaattaaga agagtattat atgaaagtaa taatttaaaa aaagaatttg taaatattga
     1621 aaaaataaaa gtagatgaac atggtaagga tatctatttg acttcaatag ctgttgctgg
     1681 ttcaaggttt agaaatagag aatatcttga aaaagaattg gagaagagta agatatacta
     1741 tttaactaga gagaaagata atctatatga tgaaaaagct attaaaatag ttggtgaaac
     1801 tggatatgta ataggctatg tcccaagaaa agaaaactat attttgagca atttattaga
     1861 tggaggaaaa ctgctgtatt gtagagtaac tgaatataat ttatatgaag attgtatcta
     1921 cgcaaatgtg tacttgagtt ataaagatgt aattgaaaca gttgaaaact ctttaaagat
     1981 ggttttagat aagagtagaa taaaacttat aaactaagac gtttatatat tgcttagatg
     2041 atgtaatcct aactgattgg ttattttttc tggaattatg gtgtatttta tgagcaatgt
     2101 taatgaatat aaaataaaaa aacaattgtt ttgtatatat gatgtgtata aatatattta
     2161 tatattcata tttacaaaac aattgaaatt tatgttattt tatgatttta aatttagatg
     2221 gaattttaac ttctgcttta tcttttattt tagaagcttg ttttttaact ttatttaata
     2281 attcagattc atctacaact aatacttttc tttccttcat caaccattta ccatcacaaa
     2341 tagtagaatc tacattttca ctagtcatag aataaactat atttccaatt ggattatgca
     2401 ttggtaaaca atgaattgta tctggattta gaataatcat atcagctttt tttcctactt
     2461 caagactacc aatttctttt tctagtaaag cacattttgc tccatttata gtagccattt
     2521 ctaagacttc ttcagctggt acgacagttg ggtttaaagt tctaccttta tgtattagtg
     2581 atgtaagata catatccctc ataaggtcca ttctattatt tgaaggagct ccatcagtac
     2641 caattgaaac aggtattcct ttttctaaca tttcaggaat tgaggcaaat cctagaacta
     2701 ctttcatagc tgcacctgga ttatgagaaa cttttacatc atgtaatcta aataaatcaa
     2761 tttctttatg agtaagccaa actgtgtgag cagctaaaag atttggtcct aatacaccta
     2821 gtttatcaag atattcaact gttccaactc cgttgttttg ctttacaaat gaaatttcat
     2881 cagctatttc agcaatatgc atatgtatac ctgtattgta tttatcagct aactctttag
     2941 tcctaataag caattcttca gttgtattaa aaatagttct taatgcaaac catattttaa
     3001 tcctctcatt agatgtgtta ttaaacttat ggaataagtc taattgagtg ttgatttcct
     3061 catcagtagt tttttgccat gcttcaggaa gaccatctcc tgtatccata actgatttag
     3121 ctaaacaggc tctaagtcca gttttatcaa cagcttcaac catagcatct acatattgac
     3181 caccagattc taaaaatgta gttaccccag attttatcaa ttcaacacaa catgcagtag
     3241 atgaaatcaa tgaatcttca tagttgaagc tactttcgta tggccagatt ctctctctaa
     3301 gccaagttaa aagtacaaca tcatctgcaa gaccacgtcc taattgttgg gataagtgaa
     3361 cgtgtgtatt gactaaccct ggcattagta tttttccttt agcatcatat atctcagcat
     3421 ctttatctac aatattaata tcaaacttac caacattttt tataatatta ttttcaataa
     3481 gtatacttcc agtttcatat acctctctat ctgcattcat agtaattaga tatgcatttt
     3541 ttataagtaa ttgtgacata agtacaacct cttttctata tatttttata tctatgtatt
     3601 atgataataa attttctata caaagttaat agcaaaatca atttatacat aaattgattt
     3661 tgctatttat aaatttaaaa tattgaccta gaactattta aaatattaac ttagaactaa
     3721 gtttacaact gcaagtaaac ctataatcca aagtgtaata ctgattttat cagtttcttt
     3781 agctaccaca tgcattatta tatatgaaat aaatccaaag cataaaccaa tacttatact
     3841 ataagttaaa ggcatcaata aaactgtaac aaaagcaggt acagctattt tgctatcagc
     3901 aaaatcaatc tgttttatgc tttcaaacat gaatacacca accattacaa gtgcaggagc
     3961 tgcagcgtaa gtaggaacta taccaagcaa tggtgtaaat ataagtgtag ctaagaataa
     4021 tgctgctgtt actattgaag caagtcctgt tcttgcacct gctgcgatac cagaagcaga
     4081 ttctccaaat gttgtaacag tactagttcc taagaatgag cctattaaag tagaagaaac
     4141 atctgcatat aacattcttc ctaaatcttt tatttcacca ttctcatctt caagacccat
     4201 ttgtttacaa caagtaataa gaaatgctaa tgaatcaaac atgtctataa acatgaaaga
     4261 gaatataggt ccaattaatg agaatttcaa tgcacctaca atatctaatt taaaagcaac
     4321 tggtgctata ctaggaggtg ttgagattat tgctgttggc aactccacaa atccaaatat
     4381 aacagatatt attgttgcaa ctactatact tatcaatatt ccacctttaa cttttttata
     4441 ttctagtatt gccattaaga taagagcaaa aagagctatt aaaactgttg gtgtaaaatc
     4501 tgcaagtgaa actatagtat ctggattagc tattataaca cccatatttt ttaaaccaac
     4561 aaaagcaagg aaaagaccta tacctgctga agatgctact gccaattctc taggaattgc
     4621 gtctgcaatt gtctttcgaa gacctgttac tgcgagtgta aagaagacac atccagatag
     4681 aaaaactacc ccaagacctg tttgccatgg gattcctgca cctataacca aagagtatgt
     4741 gaaaaatgca tttaatccca taccaggagc tagagcaaat ggtgcatttc ctaaaatcgc
     4801 aaatataagt gtaccaatgg ctgtagctag tatggtaact gttataaggg cggctttatc
     4861 cattccagct tcagataata ttgctggatt tacgaaaact atgtatgcca ttgttaagaa
     4921 tgtagtaact ccacctatta cttctgtttt aaggctagag ccacgttcgc taaatttaaa
     4981 cattttgtct aaaaagctta atgatgaatt atttccatct ggttgtacat tgttagtgtt
     5041 tggtaccatt tttacctcca aaaaacttta agatataaac aaaaaaatat atattcatat
     5101 agatagaata gaacatatat tcaaagttga tattctattc atagaaaacc catttaaggt
     5161 gagtttgtag aaactattag accgtattac taatattata tgaacatttt ctatttgttt
     5221 ttctgttgta ttatatcgta atattgtatt atttacaata aatataataa aattattcgt
     5281 ttttttgtcg attttataaa aaattaaggt tatttattgg caagaattac tgttttcaat
     5341 aattgaatat gtataagtat aatgtcaatt attctaactt gttttatact attatataga
     5401 agtctattat aaatgagttt gtgatataat tataaaagta ctgaat
//
LOCUS       DAEPOZ010000237         5400 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.237, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000237 DAEPOZ010000000
VERSION     DAEPOZ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5400
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.237"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            116..1180
                     /locus_tag="KR440_003194"
     CDS             116..1180
                     /locus_tag="KR440_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700202.1"
                     /translation="MFLKLIFKYNTLDFSRAYFDLTDWADNPKDISYYLSNGTFGDLM
                     DSIDENASKMSLSDIAKLEKKIAKKHINLWNEGKIDFSTTRFSSIVPMLQNAGVNFHF
                     IYPDIDLLKNTFNNLIKDIHLHRMKGNQPSVIYITVKDTNYDDSTLDQVYKILNSIKK
                     DKLTDFIIEKNSNSFKIFTKCETIKSLTKNFTACYIKESIENSTNIKVCVGYGIGNDI
                     KQAMSSAISANKESKINPLNYSFLVNENYETIGPLLCDKLLTVSNKVTPHINTIAKSS
                     KLSTLTIQKIISVTEILQRDEVTPKELSSCLNVTVRTANRFLSSLLKSGNATILYEKQ
                     NSTKGRPERVYKLFISENKY"
     gene            complement(1308..2498)
                     /locus_tag="KR440_003195"
     CDS             complement(1308..2498)
                     /locus_tag="KR440_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0700203.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNLPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSENGGKTLLLRADMDALPIMEETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEESTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGNVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEIIGNENVIDFPSAMGSE
                     DFSEILLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(2604..3983)
                     /locus_tag="KR440_003196"
     CDS             complement(2604..3983)
                     /locus_tag="KR440_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0700204.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELSAY
                     GHGCGHNLLCTAHVGAVVGLKREMLENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            complement(4014..5222)
                     /locus_tag="KR440_003197"
     CDS             complement(4014..5222)
                     /locus_tag="KR440_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700205.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNVLPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFRLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
BASE COUNT         1817 a          995 c          669 g         1919 t
ORIGIN      
        1 gcctgtgaag aattagtaag acatatttta gtatgcaaaa ttctatgaag caggaaccct
       61 gtgactttag tcatgggagg ttcagtttgg tactgactta caaagttact atgatatgtt
      121 tctaaagctt atctttaaat ataatacatt agatttttct agagcatact ttgacttaac
      181 agactgggca gataatccaa aagatatatc atattattta tcaaatggca catttggaga
      241 cttaatggat agtattgatg aaaatgcatc taaaatgagt ttatctgaca tagcaaaact
      301 tgaaaaaaag attgcaaaaa aacatattaa tctatggaat gaagggaaaa tcgatttttc
      361 tactactcgt tttagtagta ttgttcctat gttacaaaat gcaggagtaa actttcattt
      421 catataccct gatatagatt tattaaaaaa tacttttaat aacttaataa aagatattca
      481 tttacataga atgaagggaa atcaaccaag tgtaatatat ataacagtta aagatacaaa
      541 ttatgatgac tctactctag accaagttta taaaatatta aatagtatta aaaaagataa
      601 attaaccgat tttataattg aaaaaaatag taattctttt aaaattttca ctaaatgtga
      661 aacgattaaa tcattaacta aaaattttac tgcatgttat ataaaagaat ctattgaaaa
      721 tagtacaaat attaaagtat gtgttggata tggaattggt aatgatataa agcaagctat
      781 gtctagtgcc ataagtgcta ataaggaatc taaaataaat cctttaaatt acagtttctt
      841 agtcaacgaa aattatgaga caataggacc tttactgtgt gataagttac ttactgtttc
      901 aaataaagta actccacata taaatacaat tgccaaaagt tctaaattgt ctactcttac
      961 tattcaaaag attatatctg ttactgaaat tctacaaaga gatgaagtta ctcctaagga
     1021 attatcttca tgtttaaatg taacagttag aactgctaat agatttctaa gtagtctctt
     1081 aaagtctgga aacgctacta tattatatga aaaacaaaac tcaacaaaag gtagacctga
     1141 aagggtatac aaattattta tatcagaaaa taaatattaa aatccaaaat gcataaaaat
     1201 attttatcat ctaatattaa gtaaaattat atatgacttt ctaaaaggta tatctttgca
     1261 tctttttaag atataccttt tatgatattt aataatattt tatgttatta tctactaaaa
     1321 ttattttcta accattttac tgcacaatga gcaaataaag ctgaaccata aactaatccc
     1381 tcttcattaa aaattgcttt aggattatgc ataccaaaat tatatccctg actaatactt
     1441 cccattccaa tccataaaaa tactcctgga acccttagta aaatctctga aaaatcttct
     1501 gaacccattg cagatggaaa gtctattaca ttctcatttc ctataatttc tttaacataa
     1561 gatacaatct catctcctac atccaaatca tttataactg cgggagtccc cattttaaat
     1621 tccactttag catttcctcc aaatgattta gctattcctg tagatatttc aataattcta
     1681 ttttttaatt tttctctaac aaattggtca aatgttctta aagttcctga catagatgct
     1741 ttatttggta tagcatttgg tacgtttcct gcattaaaca taccaatagt taaaactaca
     1801 ggctctttct gaggtttttc tcttgaaagt atagtgttta atgctatttg tatatgcgac
     1861 attatgttta ttgggtccac tccaaattca gggcttgctc catgagttgc ttttccttct
     1921 actgttattt caaatatatc cgcagatgcc atagctggac cacgtgaata agctacttga
     1981 cctgtgttaa gaaatttacc caccatactg tgaaatgcca tagctgcatc gacctgagga
     2041 ttttctaata taccatcatc aatcattgat tttgcacctc tggtactctc ttcatctggt
     2101 tcaaatacaa actttactct acctttaagt aagtgcttat tatccataag tattttagct
     2161 gctcctaaga gcattgctgt gtgattatcg tgtccacaac tatgagatgc atctttattc
     2221 ttacttttaa aaggtaaatc cgtctcctcc ataataggaa gtgcatccat atctgccctt
     2281 aataataagg tctttccacc attttcactg tctaaaacag ctactattcc attattagac
     2341 gtcaatttcg gagaatatcc cattttttct aaagtactgg taacaaaggt tactgttttc
     2401 ggtagattgc aaccaatttc gggattttca tgtaaatatc ttctccatct aataatttcc
     2461 ccttctattg actgcgcttc tttaaaaaaa tttttcatat tccttcctcc taatcagata
     2521 gtcagcaaaa tctcactttc ttttagtaat gaaatgatgc tgatataaaa attataattt
     2581 tatatattaa acttcatgtc tttttaagga gttggaatat catctggaat aggacattta
     2641 tatgttttac cattcataga tttatcaaac tcttcttttg cctttttagc tatctctgga
     2701 ttatttaata ttttggctcc aaacatagcc atgatttttg atgcatatat catacctttt
     2761 tctcctatag aacttcctgc acaagatgta aactgccagc tatgacctgg tgcacctaga
     2821 ttcgtacacc ctgtaaaaaa gtatgctgtc ggaactatat gcattacatc tccaatatca
     2881 gtagaaccat aactattaaa gtttgtaaca tttcctccac cataatatag atgagttcct
     2941 tctggcaaat tatatttact tatcatagcc ttatattgtt gtgggctttg tctatctaat
     3001 tcagctgcaa aatccaattc ctctcgagtc caaggctctt gtttaatctc attcatacat
     3061 tctgatacaa gatttgctaa aacatgatta tttaaagtat tataacaacc tcctaaaaac
     3121 tctacttcaa cttcagtttc agtcatcatt gcagctcctt ttgctacctt tacaagtctt
     3181 tcatatgtac tttctacagc ttctctactt aaagctcttg tataatacca tactgatgct
     3241 ttatcaggaa ctatgttggg tgctactcct ccatctgtaa ttgtataatg aatccttaca
     3301 tcagaagtaa catgttctct caaataatta gctcccacat tagtaagttc cacagcatct
     3361 agtgcacttc ttccattttg agggtctgag cctgcatgtg cagtctttcc cttaaagtga
     3421 aattttacag agttaacagc tgttgaagta ccaattgtag cttcactaat tttatttgga
     3481 tgaaaattta tagctaagtc taacccttca aatgctccac ctctagccat aaatccttta
     3541 cctgttaatt gttcttctcc tggacatgca taaaatacta tagtcccact taagttattt
     3601 tctagcatct ctcttttaag tcctactact gcccctacat gtgctgtaca taataaatta
     3661 tgcccacatc catgaccata tgcactcaat tcaaatgctt cttgctttgt tgaaactttt
     3721 tgatttaaac caggtaatgc atcaaattct cccataaaac ctataactgg tttacctgaa
     3781 ccaaaactag ctctaatagc cgtggctaaa cctcctacac ctacttccac atcaaatcct
     3841 tcacttctta gaaagtttgc tgtattttca catgctttaa attctttaaa tgctttttct
     3901 gggttttccc atattttctt acttaaatca tcaagtttga atcttttttt atctatctca
     3961 tttatagata tacttcctat catttcttat tcctcctata agtatattta ctatcaacca
     4021 aatataattg gacctattat actagcaaat gccactgatg caatggttac agtaacaaat
     4081 cctcctataa tcatctttgg taataaataa tctgttgccc tttgtttttc ttcttcagtt
     4141 ccttccatag catcaatacc ttcgttggaa attatctgtg ttgcaggata agctagcata
     4201 caagttacac caattgcagc tgacattctt ggagaatatc ccaatagttt tcctacaatt
     4261 gcagtaaata atattataaa tacagaacct acaacaagta ttcctatagt tggaacaatc
     4321 atcccaaaca atgctgttgg agttacctta gcaagtcctc caggtaacat caacatacaa
     4381 ccaaacatta taattccatt agattgagat ttatctaaaa ttcctttttc taaaaaacct
     4441 attcgtgtaa aaatcaaccc tactaacaaa catgctatat taggatttaa ataatagttt
     4501 gcaggggtaa atcctggtat taaagtagcc aatcctacaa aattaccaat catagcaaca
     4561 aatgaaagct ttgcaagata cactagagta gatgattttt tacctaaatc ttctttaaaa
     4621 attctcatac ttggaagtct aaaattactt tttgactcat ctttcaaaaa atccccgtta
     4681 tctcttttaa attttaattc tttatttata caatatgtag ttatcggaat gccaaagaac
     4741 ttttgaaatg ataatactag aattacaaat gcagcaagtt caggtctatt tgcagcttct
     4801 gcaacagttg ttacgataat accggcaaca gtagaacctg ctattggagg tgctgctatt
     4861 aaagcatact ctctaccaaa taacatacta cccactgtta aacagccaac ccctattcct
     4921 acaataccta ctaatgcaat tacaaccgtt ttccattcat gacataagtc ttcaagattt
     4981 ataagtgttc ctaagttagt tattaataaa gctataccaa agttgctcat tgcagctaat
     5041 atccctgatg ctcctgtaat gtcttttggt aaaacatttg tccaaaatcc tagtaaaaat
     5101 ataatacata caaataacaa tgttgaaatt gcaccttttg ttttcttaga taaaaactca
     5161 ctaaaagcat aaatcattac acatacagta aagcaagtaa gtggattcca aaaattttcc
     5221 attgttgttt cctcctaaat ataataattt tttatcaata aaacaataat tataaaatgt
     5281 atattatgca gttattctta actacatatg gtatagcttg atattttttg atgatattat
     5341 atacgtctta aattattcct aaaatatcta aataaatata actagagtta actctctaaa
//
LOCUS       DAEPOZ010000238         5394 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.238, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000238 DAEPOZ010000000
VERSION     DAEPOZ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.238"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            57..1247
                     /gene="dltD"
                     /locus_tag="KR440_003198"
     CDS             57..1247
                     /gene="dltD"
                     /locus_tag="KR440_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBH0700206.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGINPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            1368..2882
                     /locus_tag="KR440_003199"
     CDS             1368..2882
                     /locus_tag="KR440_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HBH0700207.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVIFN
                     FSDERNFGDINVIDMDKLNDIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            2882..4030
                     /gene="dltB"
                     /locus_tag="KR440_003200"
     CDS             2882..4030
                     /gene="dltB"
                     /locus_tag="KR440_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBH0700208.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKHISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            4049..4279
                     /gene="dltC"
                     /locus_tag="KR440_003201"
     CDS             4049..4279
                     /gene="dltC"
                     /locus_tag="KR440_003201"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBH0700209.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            4496..5281
                     /locus_tag="KR440_003202"
     CDS             4496..5281
                     /locus_tag="KR440_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBH0700210.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPMSIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
BASE COUNT         2191 a          533 c          937 g         1733 t
ORIGIN      
        1 aaaactaata tgattaataa taacataaat ttgaatattt ttagaggaga gaaaatatga
       61 gaaaattgat atactttatt acacctttta taataggcgt agtattttta tttgggttag
      121 acaaatttct tgattctaaa actgatgaac ttttgaggga gaaaaatcta cttccaatta
      181 tggatgatac tttaagtgat ataaaagata aaggggttac agcaaataat cattttttaa
      241 gagaaaagga tattatgatt ttggggtcat cagaacttag taattcaaca aaacaacatc
      301 caaagtacta ttttaataca aataggagta aaaataaggt gtttgcaatt gggagagcct
      361 atactcagac attacaagat gctgcaatat taggaagtat gaatcctaat attgataata
      421 agaaagttgt cttgttaatt tctatgcaat ggtttatgga aaaagacgga gtcacaagtc
      481 accattatca gtctagattt tcaccaatac aattttatcg atttttagat aatccaaaaa
      541 tatctaaaca aaataagatt gaatatgcaa aaaaatcaag taagctatta tggggaagtg
      601 atgagtataa agcagaagca ttatatgcta agctatatga acctaagaca tttcctgaaa
      661 aagctgaaaa ggtgctttta gagccttatt ttcaaggaag aaagtactgt atagctctta
      721 aagaaaaggg tattctttat aaacgattaa ttaagctaga taaaaagaga gcaacaaaaa
      781 gaaaaagacc aataaattgg tcatatgaga gaaagaaagc cattgaagat gctaaaaaaa
      841 gagttggtaa aaatccacta aatattgata agtactatta taaagagcat tttaaagatg
      901 gtatagagca atacaaagga agagacaaag atgtaaattt attaacatct aaggagtttg
      961 aaaattataa actgatgcta aatgtatgta ctgacttagg tattaatcca gttgttgtat
     1021 taatcccaag tatggataaa ttctataatt tgactggtat ttctgaaaaa gaaagaaatc
     1081 agtactacga taaagcacaa aaaatagcag aaagcaaagg ctttgaagtt ctaaacctta
     1141 aagataaagg gtcagataag tattatttaa gggatgtaat gcatcttggt acgaaaggat
     1201 gggtagatgt ctgtgagaga ttatttaaaa tatttaaaga acaataaaga tttgttaact
     1261 agtatttgtt taactatggt tataatattg gcgataggag ttctaacatt tactccattt
     1321 aaagaattgc catttattta taacggattt taaaggagca ttcttatatg aaaattattg
     1381 aaggaataaa aaaatattca aatacagata gaacagcact aatgtgtaat ggagataaac
     1441 tttcttataa agatttaaat gaatattcag atgctatatc agtatttcta aaggatgtat
     1501 acaaagaaga ggatactcct atagtaattt atggaaataa agaaaacatg ataatggcat
     1561 gtatgatagg agctttaaaa tcaggtagag cgtatgtgcc tcttgatatt agttttccaa
     1621 ttgatagagt ttttgaagtc actaaagaga taaaaccaaa agtaatattt aattttagtg
     1681 atgaaagaaa ttttggagat atcaatgtaa ttgatatgga taaattaaat gatataataa
     1741 atgaatatca gggaaaatca ttagataaag aaaactgggt aaaagatgat gaaaatgcat
     1801 atatactatt tacttcagga agtacaggaa agccaaaagg ggttcaaata agttcaaaca
     1861 atttagatag cttttcagat tggataagcc catatctaaa catagatgga agtgaaaaag
     1921 taataatgaa ccaaccagca tattcttttg acctttcagt tacaacaata taccctggtc
     1981 ttatccatgg agctacatta ttttcaatat caaaagatgt attagcagac tataaagaac
     2041 tatttagaca atttagtatc tcagatatag ctgtatgggt gtcaactcct tcttttgcag
     2101 gagtatgtat aactgaaaaa gagtttaata gtaaaatgct tcctaacctt gagtcaatga
     2161 tattcatagg agaagcactt tcaaaaaatc ttactaaaga acttatgagt agatttccaa
     2221 acacaagaat tatcaatgga tatggaccaa ccgaggctac tgttggtgta agtgtaaatg
     2281 atatgactca aaaagctata gatgatgaaa aaagtcttcc agttggatac cctatgtcaa
     2341 attgtaaaat aaaaatactt gatgaagatg gaaatgaact taaagaaaat gaaaagggtg
     2401 aaattataat aattggacca tcagtttcta aaggttactt caataataaa gaaaagactg
     2461 atgaagtatt tttctatgat gaaatagatg gtgtaaagtg gagagcttat aaaactggag
     2521 atatgggata tcttttagat ggaaatatat attattgtgg aagaaaagat tttcagatta
     2581 aattaaatgg atttagaatt gaaatagaag atatagaaaa taatttgaga aaagtacata
     2641 atgtaaaaaa tgcagtagtt ttaccagtat ataaggacga aaaaattgct tacttaaaag
     2701 gtattgttga gttaaatgaa aagaatgatt taagtaatat taaaaatggg atgataataa
     2761 aaaaggaact tggaaagtat ataccttcat atatgattcc aagaaatatt tcaataatct
     2821 ctgagtttcc aacaaatatc aatggtaaaa tagacagaaa gaaacttatg gaggaaattt
     2881 aatgattttt tcacagtatg gagattattt ttatctttat atattattat taacatcaat
     2941 accagcagta attttaggtc taatgggcaa gaatataaag tattatggta tgttggcaag
     3001 tttgtttatg atattcttaa tagtaggaat tgatgtccag ctaaagtatt tggtaatatt
     3061 tattattctt gaagtaataa tagttaaagg atatgaatat gtaagaagaa aaactaaaaa
     3121 taaatacata tattggggat tcttgtttgc atcaatgctc ccaataataa taaataaaat
     3181 atctccagtt acttcttttg gaataattgg atttattgga atatcatact taaatttcag
     3241 aactatacaa atggttattg aaatttatga tggagctata aaagaagtta aaatttcaaa
     3301 gatgctttat tttatgttat tttttccaac tttaagttct ggacctattg ataggtcaag
     3361 aagatttgaa caggacttgg aaaaacacat ctctagaaaa gagtatatag aagaatactt
     3421 actgccagga ataaaaaata ttgtcatggg agtaggttat aagtttgtta tagcattttt
     3481 gataaataca tattgggtat caagaatacc taaagatatg agttttataa atattttaag
     3541 ttatatgtac gcatatagtt tatacctgtt ctttgatttt gcaggatata gtttatttgc
     3601 tataggtaca gggtacatat ttggaataca agtacctata aactttgaca agccatttat
     3661 cagtaaagat atgaaagagt tctggacaag atggcatata agtctttcaa gatggtttgg
     3721 agattatatt ttttctagat ttgttatgtc atcaatgaga aagaaaagat ttaagaaaag
     3781 gactactgct gctcatgtgg ctcagatgat aaccatgata acaatgggat tttggcatgg
     3841 tctaacttgg tattatgttg cttatggtgt ataccaagga ttggcacttg tgttaactga
     3901 tatatatcag cgaaaatcta aattttataa aaaacataaa aaagataaat ggttcgagag
     3961 agtgcagatt ttcattacat tccatattgt gtgttttgga ttgctgatat tctcaggtta
     4021 tttagcttaa ttttaaggag gaaaatttat gcaagaaaca gttatagaaa tatttgaaga
     4081 tgtattaggg actgatgaga taagagaaga tttagacctt aatttatttg agacggaact
     4141 tttagactca ttagctataa ttgaagtttt acttgaaatt gaaaatagat tagggataga
     4201 gcttcaacca actgatttag agagaaaaga tatgtctact gtaaataatt tagtaaaatt
     4261 cttggagaca agaaaataaa ttagtagtta gactttaaat attaatttat aaaataaaat
     4321 tttacaaagt ccatctgata ctaatatttg atggactttt ttaatgataa tacaaagtct
     4381 tttgaaaata actttaatta agtaaaaaat ttaatacata aatatgattt cataaatctt
     4441 aggtatcatt gtaatattat aggagaaaaa tatattatat ttaggaggaa aatgaatgtc
     4501 tttaaattcg atagatagaa aaaaagaaat acttaaatta ttagattcaa aagggaaggt
     4561 aaatactaaa gaattagtaa aaagattaga agtatcatca gaaacaataa gaagatattt
     4621 ggaagaatta gaaaatgaaa ataaattaaa aaaggtatat ggtggtgcta taaaaataaa
     4681 ttatggagtg attgaaccag aacatataaa gagaaacaat actaacctag ataaaaaaca
     4741 aagtattgct aaatacgcag ctaagtttgt aaatgacaat gagtcaatta tcatagatga
     4801 aggtactacc aatttgcaga ttgtagatta tataatatct gtaaacaatt taaaaattat
     4861 tacaaactct tatccagttc tatcaaaatt gatttcttat gagaatcaag gtttgtttga
     4921 tggagaagtt atatttattg gtggaaaagt aaatttaaaa caccaaagga cagcatgtcc
     4981 aatgtcaata gacactatga aaaacttata cgtagataaa gcttttatat ctagtgaagg
     5041 tatacttgat agttttggaa ttagtagtat agaccaaaat aaggcattgt tgtcaaaaga
     5101 atatataaaa aattcaaagg aaagttttgt attatgtgat agttctaaaa taggaattgc
     5161 tagtatgtat aagatagcag atttagagga attagatttt gttatttgtg atgtagaacc
     5221 tccgaatgaa tggaaggata agttaaatga gataaatata acatggatag aagcaaaata
     5281 aacccttagt tttatataat acaactatgc ttaatacaac taaattcata aaatatagtc
     5341 agttcataga ttaagattag aaagtaaatt ttagattgta acttaggatt ttat
//
LOCUS       DAEPOZ010000239         5393 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.239, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000239 DAEPOZ010000000
VERSION     DAEPOZ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5393
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.239"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     misc_binding    619..850
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            958..2439
                     /locus_tag="KR440_003203"
     CDS             958..2439
                     /locus_tag="KR440_003203"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBH0700211.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISSDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKKFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSEINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     gene            2449..3342
                     /locus_tag="KR440_003204"
     CDS             2449..3342
                     /locus_tag="KR440_003204"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBH0700212.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIHSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQNYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            3466..4527
                     /locus_tag="KR440_003205"
     CDS             3466..4527
                     /locus_tag="KR440_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HBH0700213.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(4682..5194)
                     /locus_tag="KR440_003206"
     CDS             complement(4682..5194)
                     /locus_tag="KR440_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBH0700214.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKEAKMAIICKKIYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
BASE COUNT         2005 a          664 c          801 g         1923 t
ORIGIN      
        1 tataattcaa aaccttattg gttatcattt acagtgtctt ctaatatatt gctttctgat
       61 atatatgata gaattgaaag tccatacata accaaaacta atgctgctgt tataagtact
      121 attcccatat ttaatacctc cttttgttta cagtatacta ttattatgtg aaatatttat
      181 gaatttaatt tgaatatata tgtaatttca cactaacaat ttcttcaaat taaacattat
      241 taactatata cttttgttga accaattatg attaatagtt taaatattag cactatttac
      301 ttatatttat atgatatacc atataattac atttttcaaa ttaaagtttt gtaaactttt
      361 tttgtaatat agtgtagtat aaaattgtaa tattacagat atttaatagt tatcttttga
      421 ttactttcat taaaaacagc ttcatcacta tgaaaatcta ggtttttaaa cttttaaaaa
      481 agttaaaaaa atatttttct aatactcaat ttgattacaa tttttttaca atttaaaaaa
      541 tattttttcc aaatttttat attaatactt gactttgtct gttttataaa ctagaataat
      601 taataatatt aagaatattg aaaggcattg attaggaaga gtaaatctga atcgattacc
      661 agagaaggaa actactagct gaaatgtttc cctaattaga aggattgaaa actacctagg
      721 agcttctatc cgaagttatc ttaatgattt tagtaggaat agacgttttt attacttgcg
      781 ttaaagtatc aagtggattt tttaaaatca atttgggtgg aaccgcggga aacagattct
      841 cgtccctttt ttagggatga gggtcttttt ttattaccta aattcggttt ttaaaatatt
      901 ctgtatctaa tttaattagc taattaaaaa tttaaaatat atggaggatt ataaaaaatg
      961 tattattcaa gtacgagagg caccgaagaa aaagtaactg catcacaagc aataataaaa
     1021 ggaatatcaa gtgatggtgg tctttatgtt ccaagtgaat ttcccaatgt aaaaaatgaa
     1081 cttataaatt tagtaaattt aacgtattct cagatagcat tttttgtact tagtaaattt
     1141 ttatgtgatt ttactgaaga tgaaattaaa aattgtatag aaaatgcata tgatgaaaag
     1201 tttgattgta gtagtatagc tccattaaat aaagtaaatg atacttattt cttagaacta
     1261 tatcacggtc caactttagc atttaaagat atggcactta ctataatgcc tcatcttctt
     1321 aaaacatcta taaagaaaga taatttagag aaagatgttg tgattcttac tgctacatct
     1381 ggagatacag gcaaagctgc acttgaggga tttaaagata tagataaaat aaagataatt
     1441 gtattcttcc cagaagatgg tgtaagccca gtacaaaaac ttcaaatgaa aactcaaact
     1501 ggaaaaaata catatgtagt tggtataaaa ggaaattttg atgatgcaca atcaggtgta
     1561 aagaagattt tttctgataa aaaatttaat caaaaactat tggataataa ttatatatta
     1621 tcttctgcaa attcaattaa tataggaaga cttcttccac aagttgcata ctatttttat
     1681 tcatatatga aattagttag caatagtgag ataaatttaa atgataaaat aaattttgtt
     1741 gtcccaactg gtaattttgg taatattcta gctggatatt atgccaaaca aatgggtctt
     1801 ccaataaaca aattgatatg tgcatcaaat gataataatg ttctttatga tttctttaaa
     1861 actggtgtat atgataaaaa tagaacattg aaattaacta cttctccttc tatggatata
     1921 ttaatttcaa gtaatttaga acgattactg tttgaaatat caaatagaaa tactgatatt
     1981 gtaaataagc tgttatcaga ccttagtgat aaaggagtct acaaaataag tgatgatatg
     2041 gaaaaaaatt tagcctcatt ctatggagaa tattcaaatg aagaagaagt taaaagcacg
     2101 ataaacaatg tgtttaaaaa ttataattat ttgatagata ctcatactgc tgttgcatac
     2161 aattgttatg aaaaatataa aaaagaaact tctgataaca caaaaactgt tattgtaagt
     2221 actgcaagtc cattcaagtt ttctgaagat gttctaaagt ctatagattg tgattttaaa
     2281 gaccttgatg actttgctat aattgataga ttatcaagta tttctaaaat agatattcca
     2341 aaacctatta aaaatcttaa aaatgcagaa atactacata aagatattta tgaaaaagat
     2401 gaattaaaac tagctataaa aaaattctta aaggtatagg tgattattat gttagagatt
     2461 atagttcctg caacaagtgc taatataggt ccaggatttg attgtcttgg aattgcgtta
     2521 aatatataca ataaatttta tgttgaagaa attgaaagtg gtcttgaaat agaaggttgt
     2581 gaagatgctt acaaaaacga aaataactta gtttatacat ctatgaagta cttttttgat
     2641 agagttaaac cagaaaaaat acctgctggt atcaaaataa aaatccacag tgaagtacct
     2701 atatgtagag gtcttggcag tagtgcctct tgtatagtag ctggagttat agctgcaaat
     2761 gctctatcag gtgctaattt agataaaaat caattattaa atatagcttc agaaatagaa
     2821 ggtcacccag ataatgttgc tcctgctatt ttaggaaata tgatcgtatc tgttactgat
     2881 aatgagaata tacattatga tattataaaa attcctgaag aacttaaatt ttgtgcaatg
     2941 atacctaatt ttaagttatc aacagaaaaa gctagaggtg ttttaccaaa ggaaatacca
     3001 tattctgatg gggtattcaa tgttagtaga gttgctttac taatatctgc cttactaaac
     3061 aaaaactttg acctacttaa agtagcttgt caagacaaat tgcatcaaaa ttatagagga
     3121 actctaattg agaactacaa tgatattgtt gaaaaatctg aacaattaaa tagtattgga
     3181 gtatttttaa gtggagctgg acctactata atgtctttaa taaaagaaaa tgatgatagt
     3241 tttgtggata atatgaaaaa ttacctacaa aaactaaaat ctgattggga aataaaagag
     3301 ttatgttgcg attctaatgg agctgtttta aatataatat aattatcgtc ttctatacaa
     3361 aatataacta ctcttttatt ataatttgta gtataataat taggactagg taataatatt
     3421 aagtattata atctagttct ttaaacatgt ttacattgag gagggattat cataaaagag
     3481 ttaataatta gagatttagt tgcaaaagtt cctataattc aaggtggaat gggcgttggt
     3541 atatctaggt catcactagc tggtgcagtt gctaagcttg gtggtgttgg tgtaatttct
     3601 ggagttcaaa ttggatatga tgaggaagat tttgaaacta atactataaa tgcaaattta
     3661 agagctataa aaaaacatat ttctaaggcc aaagaaatct caaatggtgg tattattggt
     3721 attaatttta tggtagctat gaaagaatat gaaacatacg taaaggaagc tgttaaagct
     3781 ggtgtagacc ttataatatc tggagctgga ttacctaata agctaccatc attagtaaag
     3841 ggaagtaatg taaaaatagc acccatagtt tcaactgcaa aagctgctaa tgtaatttta
     3901 aaaatgtggg atagaaaaga gaaaactact gctgacttaa tagttgttga aggtccaaaa
     3961 gctggtggtc accttggata ctctaacgag gaacttgata acatagattc tatagactat
     4021 gataaggaat ttgttgaaat tttaaaggtt gcaaatactt atggagaaaa atttggtaga
     4081 aacataccag tagttgctgc tggtggtata acttctagta gtgatgttaa aaaatatata
     4141 gatatgggtg caagtggtgt acaagttggt acaagatttg ttgctactta cgaatgtgat
     4201 gcccatgaga actttaaaat ggcatacata aatgctagtg aagaagatgt tcatatagtt
     4261 aaaagtcctg taggtcttcc tggacgcgcc ataagaaaca aatttataga agaagttaaa
     4321 ataaatcgtc cagaaataaa gaagtgctat aattgtttga ttccttgtaa tccaaaagaa
     4381 actccttact gtatttctca agctctaatc aatgcagtta aaggagatgt tgaaaattca
     4441 cttttatttt gtggaaatga tgcctataaa attgataaat taagtactgt tgaagatgtt
     4501 atcaatgaat tgatttctga attataattt aaagaaaact aaattaaaat tttatcaagc
     4561 taaggagttt taatacgtta tttattaatc tttataaaca tatttatgtc cttagctttt
     4621 ttgtgtcaat ttccttttat tgaaatttta atttaattag agatagtatt aacccttata
     4681 actaatcttc ttttacaagt acattggtta tttcagctat atataaagta tgataatctt
     4741 tttgtggata ccatcttcca tctatacctt cagcaataaa acattggggt tcaagtttct
     4801 gactataaat ttttttgcat ataatagcca ttttagcttc cttaaaataa ggtgtatcat
     4861 ttaagtgttc tatattaaat ccaatctgtg atatcttatc ctcttctcta ccagatacct
     4921 ttccacaata tgaaagctct ttacgaaaat cttcatcaaa aaatgtaaga gaaaatttat
     4981 ctgtattatc tataaattcc ttagtataac gctgtggtct tattactaca aaagcaacat
     5041 cttttcccca cataactcca agtcctcccc agctagcagt cattgtattc actttacctt
     5101 cattttctgc tgttataagt gtccaatctt tacctatcaa tccaaatgga ttatttttaa
     5161 tctcttctgc caaaatttct ttaaaatcac tcatatctct aataacctct cattctctta
     5221 tatttatatt ccttttattt tcgtgataaa ttataaatga agtctagttt tgaaaataaa
     5281 atactttttt aaatatatta ttgcaaataa gtaaatatat atatacactc ttatttttta
     5341 atatatttaa taatttcact cagaatatat atattcttta ctcaaattaa ata
//
LOCUS       DAEPOZ010000240         5378 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.240, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000240 DAEPOZ010000000
VERSION     DAEPOZ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5378
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.240"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            69..872
                     /locus_tag="KR440_003207"
     CDS             69..872
                     /locus_tag="KR440_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700215.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            884..2014
                     /locus_tag="KR440_003208"
     CDS             884..2014
                     /locus_tag="KR440_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBH0700216.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLRESIDEPIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEGFVKSYFESYSLIRG"
     gene            2018..3427
                     /locus_tag="KR440_003209"
     CDS             2018..3427
                     /locus_tag="KR440_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700217.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDMDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYVIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDVEIDYSP
                     ERTHDIWDESNEIDKNTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKAEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            3638..4147
                     /locus_tag="KR440_003210"
     CDS             3638..4147
                     /locus_tag="KR440_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBH0700218.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPI
                     YSNNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLSEEFATILDISPNEVS
                     SYISSHIPQ"
BASE COUNT         2252 a          454 c          898 g         1774 t
ORIGIN      
        1 atataaatag gaatagatta ttataattaa tatagatata aaaataatta tagaaagaaa
       61 ggcacattat ggaaaaattg agagttattg aaatgataaa aaataatccc aatattatag
      121 ctacaataga taatccaact gatgaaatga aattgttagc tattaaagaa aatgggcttg
      181 tactagaata tataaataat ccaaccagag agatgcaaga actagcaata gataataata
      241 ttagagcaat taaatttata gataatccaa cagaagatat gatgataaaa gcagtaaatg
      301 agggatggag tatactggat tatatcaaaa acccaactga taaagttatt gaaatggcta
      361 taaatcaagc aggatgggca attaaatatg ctaaaaatcc aagtgaagaa ttacaactgt
      421 tagcagtaag aaaaaattat gattcgataa agtttataaa acaaccttat gaaagtgtac
      481 aagaggaggc agtaagaata agttatgatg ccttgagata tatagattca cctagttata
      541 atgcagaact tatagctata aaaaataatg aagcagctat tagctttata actgatttag
      601 ataaaaataa aatgttggaa tttttaaaag tgaatatttt agtgattaag tacattctga
      661 aaaaagtatc taaagatgaa ttagagagtg tattaaaaga agtattatcc aaagaagatg
      721 ttgaggaaaa gtatattaga gattttttga attgtagtgt tatagataga aatagcgaaa
      781 gtataccaat agataaaatt atgtttattt ataaatatgg aagtaagaca gcaaaaaaaa
      841 tagctgtaga tgagaaatta aagattatgt aggagagaga aaaatgaatt ttatagacac
      901 attaaaaagc gttgacttaa tattatccac tggagaagta cctttaatag taggtgaaag
      961 tggaatagga aaaacagctt tagcaaataa gcttgccaaa gaaaatgatt ggagtttaat
     1021 tgttattgat ggaaacttac ttaaagaggg tgaaataggt ggtcttccaa ctatagaatc
     1081 ttatgtagga gttaattcta atggatataa gactgaaaag aaaacaacag tgtatgctgt
     1141 tcataacaaa ttaagagaaa ttgatgaaga gatatctaaa agtaagacag ttcttttatt
     1201 tatagatgag ataaatcgtt gtgagcatac agtacagcaa gaacttatga atttaatttt
     1261 aaatagagaa attaatggat ataaactgca tgatgatgta aagattttag cagcaatgaa
     1321 tccatcaagt aaatatggtt cagattttga ttatcaagtg gtagatatgg atgcagccca
     1381 agaaaatagg tttgtttggt taaatatgga gagtgaccat acacaatgga taaaatgggc
     1441 aatagatgag ggtattgaaa gaaaagttat cgagtttatt tccacttttc cagaatatct
     1501 gcataaaata aatgaagatg atgtaagagc tactcctaga agttatgaga gagtatctaa
     1561 aatttataaa gtttataaag aaaaaaataa ttctattcct agagcagtat ttttaaatgt
     1621 tgtaaagggt aatgtaggta aagttattgc agaagaattt ataagtttta ttgaatcaaa
     1681 ctctgaacca ttaatatcct atgaagatgt ttttttaaga gaatctattg atgagcccat
     1741 tgtagaaaga gtaaaaaatg agagtcatac aagactttat ctatcagcta tgaacattct
     1801 aaaagactta gaattaaata ttaagaatga taaatatgaa tcaaaccact atattaatag
     1861 atttattgaa tttttaaaaa tgtatcctgt agatttaatg ataggtatta tgaaggatat
     1921 tagaaacagt tatattgaag tatataaaaa agccatagaa aatgaaggtt ttgtaaaatc
     1981 atattttgaa tcttatagct taataagggg ataatgtatg gaaaattatt ttgataaaca
     2041 agcaaaagag ctttataata aagcaagtga aattataaat actcattcta tgctaaaggc
     2101 aaatcgtgct aatgagaaat ttgatatgga tataccacaa gattttaagt atgagttttt
     2161 tagtcttgta gataaagtga atttaagtct tatggaagaa gaagataatt tttatggata
     2221 ttttttgttt cagatgtcaa gagaaataag atttgatata agtagtccta caggcgtgaa
     2281 ttttaaggga gctaaatatg ttatatattt taatccaata atctttttaa cgcttgacat
     2341 aaagcaaatg gagactacaa ttaaacatga aattcatcat atattatcta tgcatttgat
     2401 gagagcaaag gagttaaaag gaaagtatag tacattggca attaatatgg caatggatat
     2461 agtagtaaat aaatttctaa acaatttacc accatatgca actaccttag agtgggtaaa
     2521 tttaaaatat tccttaaaac ttgaaccata tgaacctttt gaatattatg tagaaaagat
     2581 tcagactgaa ttagatttga tggaagtgga tgaagatggt gaggaagatg atagtgataa
     2641 atatggagat gtagaaattg attatagccc cgaaagaact catgatatat gggatgaatc
     2701 caatgagata gataaaaata cccttagaga atttactgaa aaatttatta acagttctca
     2761 aaaaggtaaa gttcctgctt atttagaaag tatgatatca tcacttaaaa atagtaaggg
     2821 tgaattgcca tggaatttat atcttaatag aataatggga acagttgaaa ataataaaaa
     2881 gaagactata acaagaagaa atagaagaca accaaatcgt ttagatttga ggggagaact
     2941 tagaagtcat aaggcagaaa tagcagttgc acttgatgta agtggaagta ttagtgatga
     3001 agaatttaaa caagctatta aagaagttct tagtatagtg aaaaactata atcatgaaat
     3061 tacaattata gaatgtgata atgaaattag acgtgtatat aaagtgagaa ctgaaaaaga
     3121 tataaaagat agaattagga taggtggagg tactagattt tctccagtct ttgagtatgc
     3181 taataataaa aagattaatt tgttagtcta ctttactgat ggtaaaggtg aaaataaact
     3241 acaagtaata cctagagggt ataaaatttt atgggttatt tctggaagag gagataaaat
     3301 ttcattaaaa gaaccttatg gagcagttaa aaaacttact aaggttaaaa caaatgatga
     3361 aatattagaa ataaatgatg taagaaatga tgggtactca atgaatagcc aagcaccaat
     3421 tttataggta ttcacaggag ataagctgta ttaatttatt ttgatatgct tgttataaaa
     3481 atctaaaaaa tacattaatc aatggttaat gtatttttta attttaataa tataagtttt
     3541 acttacattt aagtcttaaa atgtgttaaa atataatata tagcagatag ttttggcaag
     3601 atggatttag ttgtcaaaac tatttttttt attaaggatg gtgatgtttt tgtttaagat
     3661 tgatgattat ataatgtatg gtatgacagg agtctgcaaa gtcatggata taacaaatga
     3721 acgatttaca aatggaataa aaaaagagta ttatgtattg agtcctattt attctaataa
     3781 tacagtaata aaaattcctg tagataatga aaaagtacca atgagaaagt tactttctaa
     3841 aattaatgtg ctttcattaa taaatgatat tcctaatatg gatacatcat ggatagataa
     3901 tgaaaaatta agaagtgagc aatttaaaaa aatactcaga ggtggtaaat gtgaagagtt
     3961 aataaaatta gtaagaagta tagataataa cagagagtat gtaaaatcta ttggtaaaaa
     4021 aacacatcaa gcagatgata atataatgaa agaagcagaa aggttattaa gtgaagaatt
     4081 tgcaactatt ttagatattt ctcctaatga agttagttca tatatttcaa gtcatatacc
     4141 tcaataaagt gttttagtat cctttattta atctatttta agtattattt atgtatgctg
     4201 gttttggtaa aataaaatat ataattaaat tatataaaaa attaagaatg attgtattga
     4261 agaattacag aatagtgcgg agagcccact cctttaggtg tgagatggat atcacttaaa
     4321 gatttaaatt gttattgtag atgtttttaa tataaagcaa gattaataag tgtattgtaa
     4381 gctttaagtg aaattgatac tataatgagt tacttagatt ttaaaatata taatattata
     4441 aaaaatcctt ttaggttttg aatgaataag atggatacta tgttgaatag aatccatctt
     4501 atttaagttt tatttatgtt gtttatttgt aagcattatt tttgtacatg aaaacacagt
     4561 gtaaaaatat gcttagaatt atgaagtttt attaaaaata tattcgacaa aataagacta
     4621 aaaatattct taaacttata actaagataa atattgtata tttatagtgt agcctgaaaa
     4681 attgtggatg tacgaaattt atttagtatt ttattgaaac aaagtagaag aagtactata
     4741 gtttatcttt gaatagattg ttatttttta gtgaaaaagt tattaggtta atacataaat
     4801 ataatagctt ataaatagtt taagcatgtt aaaaaagtag ttattagaga tatatagtta
     4861 cattgcttat aagatactta aaatgatagc attcacttat atttagaaaa ttaagataaa
     4921 aaatcatttt tatatagtaa agcataccta aatttagaaa ataattgaat ttataaaatt
     4981 aattaaatac ataaaaaagt attcaattaa cttaatagat tttttaaaaa ttaatttaaa
     5041 ttttttcttc tattaactat taatattatt ttcaaatcaa cgcagttata agaagtgtat
     5101 aatttgatta ttatatactg catacaaaaa tatatttgag caaataagta aagtaataaa
     5161 aaaatcaaga tttcatatct aaaaactatt ctgaacatta atttaattta tattttcata
     5221 ggaaatattt tatataatac acttaacaaa tataatttaa atttttctaa ttatttactg
     5281 tcgattaata gatttttttc ttgcatataa gtgcatattt atggtacgat ttacatggta
     5341 aaataaaaaa ggggaggtga aaaataaata ttcgtgaa
//
LOCUS       DAEPOZ010000241         5350 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.241, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000241 DAEPOZ010000000
VERSION     DAEPOZ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.241"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            70..771
                     /locus_tag="KR440_003211"
     CDS             70..771
                     /locus_tag="KR440_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HBH0700219.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIINIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDDLCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            1042..1860
                     /gene="larE"
                     /locus_tag="KR440_003212"
     CDS             1042..1860
                     /gene="larE"
                     /locus_tag="KR440_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HBH0700220.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNMDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKILGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLFNLGFSQFRVRM
                     HGEIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            1915..3063
                     /locus_tag="KR440_003213"
     CDS             1915..3063
                     /locus_tag="KR440_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBH0700221.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            3199..4587
                     /locus_tag="KR440_003214"
     CDS             3199..4587
                     /locus_tag="KR440_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700222.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYTTLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            4616..5314
                     /locus_tag="KR440_003215"
     CDS             4616..5314
                     /locus_tag="KR440_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0700223.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     ENDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
BASE COUNT         2173 a          488 c          975 g         1714 t
ORIGIN      
        1 ggttttaaaa atctgacaac aaaaataaaa ctttcaaaaa attaaatcta aaaagaatag
       61 gagataaata tgagtattga agatttatat gattttgaat gtgttccttt gaacaatgag
      121 attgatattg atgttttatt tgaaaagatg atagaagatg gaaaagaaaa agaatataca
      181 ccaattatag tcattgatga aaaagtaggg ctattaagaa aaaatataga tatgattact
      241 aaagagtatg gttctcttga aaactataga gagtattgtt taacaaaata taatgaaatt
      301 gatgtaaatc aattttttga ttttaggaag agtgagattt cagaattatt aaaggagcta
      361 attgatttag aagatgattt ttatgattat aaacatttta gaccaattat aaatatagat
      421 atatttcatg aatataacaa agtttacata gctaaaattc caacaattaa accatatgaa
      481 gtatttgcat acatacctat tggaggattt aatgattgtc ctagagatga agaacatata
      541 gctattgcaa aatattggta tgaaaagcat ggaatctttc caatcgcaat tggatgtgat
      601 acagttcaat attctgtaaa aaatttaact aaagatggta aaaaatttga tgacttatgt
      661 attgaattag tattttattg tgaggatatt attatacaag ggtatgaaac cttaaaggct
      721 cttaaggatg tattaaaacg ctctacaata gtgctttttt ggtgggattg atattctatc
      781 atttagatag tattaaattg ttagtcacca agaatccact tactttagtt atggggagtg
      841 tgtcaaacta ggtttttata cacagtgtag catagtaata tgtttaaatt acaaaggtta
      901 aaaaaatact ttaatatttt taatattata attaatagat ttaaaggtaa ttataattta
      961 taaaataaat actttatatg ttataatcat taagaatgta aaaaaataat atatagtttt
     1021 atagttcata tttttccaga tatggaggta aattttgtga aggaaaaaga aaaattagat
     1081 aaattaaaaa aaatgttact tgaattaggc agtgttgtag ttgcttattc tggtggagta
     1141 gatagtaatt ttttgcttaa ggtcgcaaaa gatacattag gtgaaaatgt tatagctgtg
     1201 accattcatg caatgatgca ttcaagtagg gaaattgaag aggctaaaca atatactcaa
     1261 aattttggcg ttaaacatat catattaaat atagaaaact ttgatttaaa agaatttaaa
     1321 gaaaatggtg ttgatagatg ttatcattgt aaaaaatata tattctctaa aataaaagag
     1381 gtagcaaaag aacataatat aaagtacata gtagatggga caaatatgga tgatttagga
     1441 gactatagac ctgggcttaa ggcattgagt gaattaggag tgattagtcc attaaaagat
     1501 agtagtttaa aaaaagaaga aattaggtcg ctttcaaaaa tattaggatt aaatactttt
     1561 aataaaccat catttgcatg tcttgcaagt aggattcctt atggagttga gataacagat
     1621 gaaaatttaa gaatagtaga aaagagtgaa gaatatctat tcaatttagg tttttctcaa
     1681 tttagagtga gaatgcatgg agagatagct agaatagaag ttgggcagga agaactaggt
     1741 aaattttttg aaaataacaa ttttaataaa gttgatacta aattaaaaat atttggcttt
     1801 aaatatgtta cactagatat gtcaggatat aaaatgggaa gtatgaattt aaatgtgtag
     1861 attattttaa aaataaataa gttttatagt taatataagg aaacaggagg ttttatgaaa
     1921 aattatacat taatatcgcc atgttttttt ggaatggaaa aaatgctggc tagagagatt
     1981 actaatttag gatatgaaat aataaaaaca gaagatggaa gaataacata taaaactgat
     2041 gagtttggaa tagcaaagtc aaacatgtgg ttgagatgtg ctgaaagagt acatcttaaa
     2101 attgctgaat ttgaggcaaa aagctttgat gaattatttg aaaatacaaa aagaataaac
     2161 tggtctagat atatacctta tggagctcaa tttcctatat caaaagcttc ctctataaaa
     2221 tcaaaattat acagtacacc agatgtacaa gccatagtaa aaaaggctat agtagaaagc
     2281 ttaaagaaaa gttatttaga ggatggtttg cttaaagaag ataaggagaa ataccctatt
     2341 tttgtattta tacacaaaga taaagtaacc atttctatag atacaacagg agatgctttg
     2401 cataagagag gatatagaga aaaggcaaat aaagctccaa taagagagac tttagctgct
     2461 gggcttattt atttaactcc ctggaaagca ggtagagttt tagtagaccc tatgtgtggt
     2521 tctggtacta tattgattga agctgctatg attggtataa atatggcacc tggcttaaat
     2581 agagagttta tatcagaaaa gtggagaact cttgataaga aaatatggtg ggatgtaaga
     2641 aaagatgctt ttaataagat agataatgaa tctaaattta agatttatgg atatgatata
     2701 gatgaagaat ctatagatat agctagagaa aatgctgaaa tagcaggtgt agatgaatat
     2761 atagagttta atgtaggaga tgcaactcaa tttaaaagtg aagatgagtt tggatttata
     2821 atcactaatc ctccttacgg agaaagatta gaagataaag acagtgttaa acagttgtat
     2881 aaggagcttg gttatgcatt tagaaaatta aaaaattggt cgtattattt gataacttct
     2941 tatgaagact ttgaatatga gtttgggcaa aaagcagaca aaaaaagaaa attgtataat
     3001 ggtatgttaa aaactaactt tttccaatat ccgggtccta agcctcctag aaataataaa
     3061 taagttttac atgatttatt tgtaaaatat aaaattttat taaaataagt aaaacattat
     3121 gatgccttga aacgtctaat tattgtgagg agatttttgt gaacaaattt aaagatttat
     3181 aggattagga ggagttaaat gaaagtgtta aagcttatta ctgtagtagc cctgatgatg
     3241 ttatttgtta ctggatgtaa tataaaaact gaatctccag aacaattagc gaagatacct
     3301 gactatgatg atacaaaaaa agtattgtat gatggtattg accagttact aaaaccagat
     3361 tcatctatga tattgccttc taatacaaaa gaagtaggaa aaataaataa ggtagattta
     3421 aacagcgatg gaattgatga attagtagta tttgaacaaa aagaagattt aagtaataat
     3481 gtgagtcagg tagggtttgt aaccttgagt tacaatggag aaaaatacgt acttggagac
     3541 cattttttag aaaatggcga atcaatagaa tatgctaatt tttatgattt ggactcagat
     3601 ggatataagg aagttatctt attggttaaa tccaaagaca aaacgaatat gcatatatat
     3661 aaagtagtag ataatgaaat aacaaaaata tatgacttgg atgcatcatg gcttcaaaat
     3721 aaagaagatt ttaatgacat gaaagtaaaa attggatata tagatggaga tgacaagtta
     3781 gacatattga tgcttcattt gaataataag actaatgaaa tgtttgcaag tgttgcaaat
     3841 tttgatggga aaatgaaaat caaagattct gttaagtttg aaaatgtaaa aaacttaagt
     3901 gaattatata caacattagg taatgttgct tcatctgttg gaaattcttc tgcagctata
     3961 aaaggtattg tacttgatat tcctatattg aaagataata actatattac gcagatactt
     4021 tatatgaaag atgggaaaat taacaaagct tttagtgatt atgataaaac tattactaaa
     4081 tcttattata tacctgtaga tgatattgac aaagataaga ttatagaaat acctatagtt
     4141 gcaggcagta caggtaataa taaaaatact tatagttcta aatcatctgc aactataagt
     4201 tggtatagat ggaatggaaa agaaggtgct gactcaagtt taatatttac aagtcagata
     4261 tattataatt ataaatataa ttttaaatta tttattccaa ataatttatt tgataaaata
     4321 cttgttgaac aggaatttgt tggagaaaaa gcagtattta aattttacta ttttgactat
     4381 tcagatcgta agaatttatt taatatagta gtagagagta aaaacaaact tgaagataga
     4441 aaaaatacag gtactcaaaa ctctattgta cttcaagaga gcgatgaata tactttctta
     4501 ctagttgtaa acaatgcaaa tgaaatggat aagctagata tgactataga cgcattaaaa
     4561 gaatatttct cattaatata tggttaatat tatactcaat tggaggtttt attttatgaa
     4621 ggaaaagata cttatactag aagatgaaat tggtattaga agttttgtta gtataaactt
     4681 aaaaagagaa ggctatgaaa tagttgaggc aggtactgga agagaagcta tagaaaaaat
     4741 gacaacagaa aatgatatta caatagctct tttagatgtg atgcttccag atataagtgg
     4801 tatagaagta tgtaaattta taagggagaa ttttgaccaa gtaggtataa taatgcttac
     4861 tgctaaagct caagaggatg ataaaataga aggatttatt tcaggagcag atgattacat
     4921 cataaaacca tttagtataa aagagctttt agtcagggtt tctgctcttc ttagaagagt
     4981 agcaaaggat gattcaagtg ttaagtcgag tgaaatagtt tctccaccat ttatacttga
     5041 tatagacaag agaaaattat ttaaaaatgg aaaggaaatt gagcttacac caactgaatt
     5101 ttcaatagtg aaatatctaa tatcaaatgc taagcaatcg ttaagtagag accagatatt
     5161 agacgaagta tggggaacta actatttata tgattttaaa atagtagatg taaatataag
     5221 aaggataaga aataaaatag aagatgaccc atcaaagcca aaatatatac aaactatttg
     5281 gggttatgga tactgtttta gaaaggaaga ataatagtgt tacattttga aggtctgaga
     5341 aaaaaagtaa
//
LOCUS       DAEPOZ010000242         5346 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.242, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000242 DAEPOZ010000000
VERSION     DAEPOZ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5346
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.242"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            35..415
                     /locus_tag="KR440_003216"
     CDS             35..415
                     /locus_tag="KR440_003216"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBH0700224.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRLFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
     gene            430..891
                     /locus_tag="KR440_003217"
     CDS             430..891
                     /locus_tag="KR440_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBH0700225.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            912..1502
                     /locus_tag="KR440_003218"
     CDS             912..1502
                     /locus_tag="KR440_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700226.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            1517..2674
                     /gene="alr"
                     /locus_tag="KR440_003219"
     CDS             1517..2674
                     /gene="alr"
                     /locus_tag="KR440_003219"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBH0700227.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            2707..2994
                     /locus_tag="KR440_003220"
     CDS             2707..2994
                     /locus_tag="KR440_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HBH0700228.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            2963..3346
                     /locus_tag="KR440_003221"
     CDS             2963..3346
                     /locus_tag="KR440_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBH0700229.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            3517..4344
                     /locus_tag="KR440_003222"
     CDS             3517..4344
                     /locus_tag="KR440_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBH0700230.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            4337..5278
                     /locus_tag="KR440_003223"
     CDS             4337..5278
                     /locus_tag="KR440_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBH0700231.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGINAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEAIIAKRM"
BASE COUNT         2128 a          567 c         1076 g         1575 t
ORIGIN      
        1 ttgaaagaag tattataaat tttctggagg aacaatgaat atatttgata ttggcgtaga
       61 tataattgaa attgatagaa taagaaaggc tgtagacaaa aataataggt ttttggagaa
      121 gatatttaca gatagagaaa tagagtattt taattctaag aattttaaag cagaaagtat
      181 agcaggtaat ttcgcagcca aggaagctat aagtaaatct ataggtacag gaattagatt
      241 gtttaatttt aaagatatag aagttctcag aaatgaaatg ggaaaaccaa tagtaaaaac
      301 ttataataat ctagcaaaaa tgtgcataga ttataatgtt ttagaaataa aagtttcaat
      361 atcacattca aaagattatg ctatagcaaa tgcgataacc ataattaagg actaaggagt
      421 gattacatta tgatggataa aacagcaaaa gaaataatga caacagatgt aatagtagca
      481 aaacaagatg atagtatagc agatgtagct aatatgctaa ttgcagaaaa aataggaggt
      541 ctgccagttg tagattcaga gaatagagtt gttggaataa tttctgaaac agatattctt
      601 aaaaaagaaa aatatataga agcacctctc tatataaatc tattacaagg attgattttc
      661 ttagatgact taaaaaaagt tgaaaaagat ataaaacaag tagctgctta taaggttgga
      721 gaacttatgt ctaaagatat aataaaggtt catgaagatg ataagtttga tgatgtagct
      781 aatgtaatga ttaaaaaatc aataaacaga gttcctgtag tagatgatga caacaaactt
      841 aaaggtataa tttgtagata tgatataata aaagctttat acaatgaata aactgaagta
      901 ggaggtgtga tgtgagaaaa aagtggacca tagtatgtat tatgttcttg gcactggtta
      961 taattgtaat aggatgccaa aaacgacagt ccacaaaaga agaagtatac aaagattttc
     1021 aaaagcagat ttctgatatg aattattaca gttgtaaagc tgaagtagaa gtagttggaa
     1081 ataaaagccc acacaattat gttttaattc atacttataa aaaaaccgat aactataaat
     1141 tagaagtaat ttccccaaaa catttaaaag ggaaaagtat agaatatcaa ggtgataaaa
     1201 tattagttaa aaatccaaag attagtgatg tagtggagtt acctaataca ggaaaaaata
     1261 atcaatatct ttttgtagga gactttataa aaaactatct tcaaaatgag gagatgaaag
     1321 taaagttatc caaaggacat ttggttttag agacttttat tcctggggat aataaatatt
     1381 ttaacaagca agtattatat gtaaatgctg acacaaaaaa tcctgaaaaa atggaagtgt
     1441 tagataaaga gggagtgcca agatttacag taaaatacaa agattttgaa tacagaaact
     1501 aagggggact aaagacatgc aaaaaataac agtgcctaca tgggcagaga taaatctaga
     1561 taacttaaga tttaacttaa ataatattaa aaatttatta gaagaagata ttaagatttg
     1621 tggagtaata aaagctgatg catatggaca tggtgcagta gaagttgcaa aattgctaga
     1681 aaaagaaaaa gtagattact tagcagtagc aagaactgct gaaggaattg aacttagaca
     1741 aaatggcata acacttccta ttttgaactt gggatatact ccagacgaag cttttgaaga
     1801 ttctataaaa aataaaataa ctatgacagt ttattcttta gaaacagcac aaaagataaa
     1861 tgaaattgca aaatctttag gagaaaaagc ctgtgttcat gttaaaatag actcagggat
     1921 gactagaata ggtttccaac ctaatgagga gtcagtacag gaaataatag aattaaataa
     1981 attagaatat atcgatttag aaggtatgtt tactcatttt gctacagctg atgaagtaag
     2041 taaagagtac acttataaac aagctaataa ttataaattt atgtctgata aattagatga
     2101 ggctggtgta aaaatagcta taaaacatgt atcaaacagt gcagctatta tggattgccc
     2161 tgatttaaga ttaaatatgg taagagcagg aataatatta tatggtcatt atccatctga
     2221 tgatgtattt aaagatagat tagaattaag accagccatg aaattaaaat caaaaatcgg
     2281 acatataaaa caagttgaac caggtgtagg aataagttat ggactaaaat acacaactac
     2341 aggtaaagaa acaatagcta cagttccaat aggatacgca gatggattta ctagaatcca
     2401 aaaaaatcca aaggttctta ttaagggaga agtgtttgat gtagttggta gaatatgtat
     2461 ggatcaaata atggttagaa ttgacaaaga tatagacata aaagttggag atgaggttat
     2521 actatttgga gaaggcgaag ttacagctga gcgtatagct aaagacttag gaactataaa
     2581 ctatgaagtg ttatgtatga tatcaagaag agttgaccgt gtttatatgg aaaataatga
     2641 gcttgtacaa ataaacagtt atttgctaaa ataaaaccat gatattataa tctgggaggc
     2701 gattttgtgt acgataagag taaagaaaaa atagaagtta ctttacctga ctctctgatt
     2761 tctgaagttg atagtatagt tcagatggag aacagtaaca gagaagattt tgcaaaagct
     2821 gcttttcagt tctatataac tcagaaaaag aaaattcata ttaaagaatc tatgaaaacg
     2881 ggttatagag aaatgggtca aattaatctg tcactagctg aattaggtat gacagttgaa
     2941 gaagtatctt ctgatgataa aaatgaaggg aagattgccc aaggtgagta ctaattgtga
     3001 aataaagcgt ggtgatttat attacgctga tttaagtcca gttgttggtt cagagcaggg
     3061 aggcgttaga cctgttctga taatacaaaa tgatgtaggt aataaatata gtcctacagt
     3121 tatagtatca gctataacat ctcagattaa taaagccaaa ttacctactc atgtagagat
     3181 aagttccaat gaatatgggc ttaataaaga ctcagtaatt cttttagagc aaattagaac
     3241 tatcgataaa aagagactga gggaaaaaat tggttgtcta gatgaaaata tgatgataaa
     3301 agtagatgat ggacttcaaa taagtttagg attgtttact ttttaaagag ggatttgtct
     3361 tttattagac aatccctttt ttcttttaaa tatctcttaa ggtttaattt taatgtacta
     3421 tgttaataaa attaaaaata acgcaaaaaa atagcataaa agtgatataa taatatggaa
     3481 ttgatataaa attacgaact caagggaggt aattgcatga gagaccataa aggattaaat
     3541 gagattgcgc gtatcataag aagagatata gtttcaatga tacatagagc caaatctgga
     3601 catccaggag ggtcattatc agtagttgaa atacttactg cgttatactt tgatgaaatg
     3661 aatgtggatt cttcaaatcc taaaatggag gatagagata gatttgtatt atcaaaagga
     3721 catgctgcac cagcattata tgctacattg gctgaaaaag gctattttga taaggaagaa
     3781 ctgaatggat taaggaagat aggaaggatg ttacaaggtc atccagacat gaaaggcacg
     3841 ccaggtgtgg aaatatctac tggttcttta ggtcaaggtt tttcagtagc atgtggtatg
     3901 gctatggcat caaagttgga taatgctccg tggaatgtgt atactttgct tggtgatggt
     3961 gaggttcaag aaggaattgt ttgggaagct gctatgagtg ctgcacatta taaacttgat
     4021 aatttaattg cttttttaga taataatgga cttcaaatag atggagatat agaaagcgta
     4081 atgagtttag gttcaatagt tgataaattt aaagcttttg gttggaatgt aattgagata
     4141 gatggacatg attttgacca aatatttgcg gctcttgata tagctaaatc aacagttaaa
     4201 aaacctacaa tgatagttgc aaaaactata aaaggcaaag gaatatcttt tatggaaaat
     4261 caagcaggat ggcatgggac agctccaagt gatgaggagc tagaaaaagc cttgcttgaa
     4321 ttaggaggtg ctgacaatga gtaaaatggc gacgagagaa gcatatggta aagcattagt
     4381 aaaattaggt caaataaatg acaatgttgt agtattagat gcagatttat caaagtctac
     4441 aaaaacacat gatttttaca agtcatttcc agatagattt tttaatatgg gtatagctga
     4501 acaaaattta ataggcgctg cttgtggatt atcaactgct ggtaaaatac cttttgcaag
     4561 tacttttgct atgtttgcta cgggaagggc ttttgagatt ataagaaatt cagtttgtta
     4621 tccaaagcta aatgtaaaaa tatgtgctac tcatgcaggt cttactgttg gagaggatgg
     4681 agcatctcat gaaagtgtgg aagatattgc tataatgaga gctataccaa atatgacagt
     4741 tcttgttcca gctgatggag ttgaaacaga aaaaattatt tttgaaatag ctaagtataa
     4801 tggacctgtt tatgttagat tagggagaag ttcagtacca gttttatttg atgaagatta
     4861 taaatttgaa ataggaaaag gtacagtctt aagagaagga aaggatgtat caattatagc
     4921 ttgtggaatt atggtaaatg aagctctatt agcacaagaa aagttgcaag aagaaggtat
     4981 aaatgctaga gttataaaca tgtcctcgat aaaacctata gacaaagatt taatattaga
     5041 atcagcaaaa gaaacgaatg ctattgtaac tgtagaagaa catagcataa ttgggggatt
     5101 aggttctgct gtaagtgagg tagtaggaga gtcttgtcca accattgtaa agaaagttgg
     5161 aattaaagat acatttggag aatctggtac tccaaatgaa ttgttaaaaa aatatgaatt
     5221 aacttgtgat gacataataa agactgttaa agaagctatt attgcaaaaa gaatgtaaga
     5281 gatataaatt attcatgttt gttaataata taatatattt aaaagagagg tataaagtgt
     5341 tatatt
//
LOCUS       DAEPOZ010000243         5320 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.243, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000243 DAEPOZ010000000
VERSION     DAEPOZ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5320
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.243"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            68..141
                     /locus_tag="KR440_003224"
     tRNA            68..141
                     /locus_tag="KR440_003224"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:100..102,aa:Gly,seq:tcc)
     gene            150..266
                     /gene="rrf"
                     /locus_tag="KR440_003225"
     rRNA            150..266
                     /gene="rrf"
                     /locus_tag="KR440_003225"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            540..2558
                     /locus_tag="KR440_003226"
     CDS             540..2558
                     /locus_tag="KR440_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH0700232.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPE
                     YLNVWELQGITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQTRLKGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKNSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVY
                     KIGGGLTSKVMNSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSDTGTTGKPLGGIK
                     EKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNAS
                     DTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPTKDYLY
                     VLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIEDSLK"
     gene            3071..5083
                     /locus_tag="KR440_003227"
     CDS             3071..5083
                     /locus_tag="KR440_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH0700233.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTGAYEGTINYNQDPE
                     YLNVWELQGITINSKNNPKTLNGQDLEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTVNASQTRLNGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKDSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGADRHETNANVIKKFY
                     TEDEIEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSAPGNSSGGMIEKD
                     YNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPDLFTSIHYNGSTNK
                     QGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRVLPSDSTKDYLYVL
                     RSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSLK"
BASE COUNT         2170 a          644 c          906 g         1600 t
ORIGIN      
        1 gatatatatg tagtttttag ggtgctaact ctaaaaaatt agttttacta atttataaaa
       61 atttaatgcg ggtgtagctc aatggtagag ttctggcctt ccaagccagc tgtgagggtt
      121 cgatcccctt cacccgctcc aaaagattat gtggttacaa tagcagagag gatacacctg
      181 ttcccattcc gaacacagaa gttaagctct ctagtgctga tggtacttgg tgggaaactg
      241 cctgggagag taggacgtag ccacgtaatc tttttttgtt gtattaaatt taataaacct
      301 acaattgtta tatgttttta tttgtctttt atagattttt ttggcaataa atgatatagt
      361 gaagataatt aaatatattc aatatattta atttttttat tttttgtttt tataattttt
      421 ttagaagttt attttttatg taattattta taattttggg taaaaacact ttgatataat
      481 ttcaaaaatt aaaaggttaa aaaacaaagt agaaatttat gttaaataag gagattaata
      541 tgagaagaaa tacaaaatta ttaacaacag ggattctttc aatggcaatc gtcgcaccta
      601 caatggcatt tgctactgaa tctaatgcta tggaaaataa cgctgattta aatataaact
      661 tagagaaaaa aagtatcgtt ttaggtagca aatcaaaagt cagtgtcaag tttaaagaaa
      721 aaccagatgc agatagcatt acattaaagt ataaatgcta tgatatgcca ttggatacaa
      781 ctctaaatta taatcaatca actgagtcat atgaaggaac tatcaattat aataaagacc
      841 cagaatattt aaatgtttgg gaactacaag ggataacaat aaacagcaaa aataatccta
      901 aaactttaaa caaacaagaa ctagaaaaga tgggattaaa tttaaaagac tataatgtaa
      961 cgcaggaatg tataattgaa gatataactt ctagaaaaga tgtaaataaa tatttaagaa
     1021 aaacttctgc acctattaca gaacttacag gaagtgatag atacgaaaca gcagttaaaa
     1081 taagtaaaga aggctggaaa aatggttcag ataaggtagt tataataaat ggggatgtaa
     1141 gtatagatgg cattatatca actccactag caactacata taatgctcca atacttttgg
     1201 ttgaaaaaaa taatgtacct aatagtgtaa aatcagaatt aaagcgttta aatcctagag
     1261 atgtaattat aattggagat gagaatgcta tttctaaaac tactgctaat caaattaaat
     1321 cgactgtaaa tgctagtcaa acacgtctaa agggctctaa tagatatgag acatctttat
     1381 tgatagcaaa ggaaatagat aaaaatcatg atgtggaaaa agtatacata acaaatgcta
     1441 atggcggaga agtggatgca cttactatag cagcaaaagc aggtcaagac aagcaaccaa
     1501 ttatattaac tgataaaaat agtattacag acaacacata taaatggtta aagagtgagg
     1561 atttacaaaa tgcttatttt ataggtggac ctcaaatgat atcaacaaat gttataaata
     1621 aggtaaatga tataactaaa gataatgtta ctaataatag agtatatgga gcagatagac
     1681 acgaaacaaa tgcaaacgta ataaaaaaat tctatacaga tgatgagtta gaggctgttt
     1741 tagtagctaa atcagatgta cttgttgatg ctttagcagc aggtccattg gctgcgaact
     1801 taaaatctcc aatacttata acaccaaaga cgtatgtatc tgcataccat aaagataatt
     1861 tagaagctaa atcagctaat aaagtatata aaataggagg aggattgact tctaaggtaa
     1921 tgaactctat agcatcatca ttatctaaac acaatacaac tccaacagaa ccaggaaata
     1981 gtgggggtaa gacagttatg attgacccag gacatggtgg ttcggacact ggaacaacag
     2041 gtaaaccatt agggggtatt aaagaaaaag actatacatt aaatacttca cttgcgacaa
     2101 ctgaatattt acgttcaaag ggattcaatg taataatgac aagagacaca gataagactt
     2161 tatctcttgg aaatagaact gctttatcta actctttgag accagattta tttacgagta
     2221 tacactataa tgcatctgat acaactggga atggtgtaga agtattttat aagcttaaag
     2281 ataaagatgg agggactact aaaactgtag ctacaaatat attaaataga atcttagaga
     2341 aatttaatct aaaaaataga ggtgcaaaaa caagaacact atcaactgac cctacaaaag
     2401 attatttata tgttttaaga aataatgata tgccagctgt acttgtagaa tgtgcatttt
     2461 tggataatga gaaagatatg agtttactta atacatcaaa taaagtaaaa gaaatgggta
     2521 cacaaatagg taaaggcata gaagattcat taaaatagtc tacaatgaga gcatagaaaa
     2581 atttaaaata aattaatctg caattttaaa tttaaactaa agctataaga taagaatgta
     2641 attataatag taataaagac tgtatataca ttgtgcatat aagtgcgaaa tgaggtatat
     2701 acagtctttt tatgattaat tgtaaaagga tttatattat atgaagaatt tacatatctt
     2761 aaggctacag ttaattgtca agagttggag tattcatttg taatacacaa taaaagctta
     2821 attactatat tttattgtgt gtttgtgtgt ttgtgtgttt tttgtggatt tttatgtcaa
     2881 taaatgatat agtatagata attaaatgta ttaaatatat ttaatttttt tattttttga
     2941 gttttataag tttattttta tatattactt atatattatt tatattttgg gttagataca
     3001 ctttgacata ttttcaaaag ttaaaagatt aaaaataaag tagaaattta tgttaagtaa
     3061 ggagattaat atgagaagaa atacaaaatt attaacaaca gggattcttt caatggcaat
     3121 cgtcgcacct acaatggcat ttgctactga atctaatgct atggaaaata acgctgattt
     3181 aaatataaac ttagagaaaa aaagtatcgt tttaggtagc aaatcaaaag ttagtgtcaa
     3241 atttaaagaa aaaccagatg cagatagcat tacattaaag tataaatgct atgacatgcc
     3301 attggataca actctaaatt acaatcaatc aactggggca tatgaaggaa ctatcaatta
     3361 taaccaagac ccagaatatc taaatgtttg ggaactacaa gggataacaa taaacagcaa
     3421 aaataatcct aaaactttaa acggacaaga cctagaaaag atgggattaa atttaaaaga
     3481 ctataatgta acacaggaat gtataattga agacataact tctagaaaag atgtaaataa
     3541 atatttgaga aaaacttctg cacctattac agaacttaca ggaagtgata gatatgaaac
     3601 agcagttaaa ataagtaaag agggctggaa aaatggttca gataaagtag ttataataaa
     3661 tggagatgta agtatagatg gcattatatc aactccgctg gcaaccacat ataatgctcc
     3721 aatacttttg gttgaaaaaa acaatgtgcc taatagtgta aaatcagaat taaagcgcct
     3781 aaaccctaaa gatataatta taattggaga tgagaatgct atttctaaaa ctactgctaa
     3841 tcaaattaaa tcgactgtaa atgctagtca aacacgttta aatggttcta atagatatga
     3901 gacatcttta ttgatagcaa aggaaataga taaaaatcat gatgtggaaa aagtatacat
     3961 aacaaatgct aatggcggag aagtggatgc acttactata gcagcaaaag caggtcaaga
     4021 caagcaacca attatattaa ctgataaaga tagtattaca gacaatacat ataaatggtt
     4081 aaagagtgag gatttacaaa atgcttattt tataggtggt cctcaaatga tatcaacaaa
     4141 tgttataaat aaggtaaatg gaataactaa ggatagtgtt actaataata gagtatacgg
     4201 agcagataga cacgaaacaa atgcaaatgt aataaaaaaa ttctatacag aggatgagat
     4261 agaggctgtt ttagtagcta aatcagatgt acttgttgat gctttagcag caggtccatt
     4321 ggctgcgaac ttaaaatctc caatacttat aacaccaaag acgtatgtat ctgcatacca
     4381 taaagataat ttagaagcta aatcagctaa taaagtatac aaaataggtg gaggattgac
     4441 ttctaaggta atgagctcta tagcatcatc attatctaaa cacaatacga ctccaacaga
     4501 accaggaaat agtgggggca agacagttat gattgaccca gggcatggtg gttcagcacc
     4561 tggaaattca tctggaggaa tgattgaaaa agattacaat ttaaatactt cacttgcaac
     4621 aactgaatat ttacgttcaa agggattcaa tgtaataatg acaagagaca cagataagac
     4681 tttatctctt ggaaatagaa ctgctctatc taattcattg aaaccagatt tatttacaag
     4741 tatacattat aatggctcaa ctaataaaca aggtcatggt gtagaagtat tttataagct
     4801 taaagataaa aatgggggga ctactaaaac tgtagctacc aatatattaa atagaatttt
     4861 agagaaattt aaacttacaa atagaggtat aaaaacaaga gtacttccta gtgattctac
     4921 aaaagattat ttatacgttt taagaagtaa tgatatgcca gctgtacttg tagaatgtgc
     4981 atttttggat aatgaaaatg atatgagttt aataaactca tctgcaaaag taaaagaaat
     5041 gggtacacaa ataggtaaag gaatagaaga ttcattaaaa taaatggaaa taaaaatatt
     5101 taaaaactta atgtaaattt atactaaagt caaaaaatga aatataagtg tatcaatagt
     5161 aagactgtat gtacatttgc acatgtgaat gcaagtatat atacagtctt tttttgtagt
     5221 taaatgcaat aatttgattg tgttttgtaa aatacacaat atgatataat tagataatat
     5281 acacttaatt taattgagga aggtgattat atggaatatt
//
LOCUS       DAEPOZ010000244         5272 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.244, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000244 DAEPOZ010000000
VERSION     DAEPOZ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5272
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.244"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..1197
                     /locus_tag="KR440_003228"
     CDS             <1..1197
                     /locus_tag="KR440_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH0700234.1"
                     /translation="SLCNIKIGECECSFENYECSIEYLNEVISNKKYRNVNIIQKYRA
                     SNNITKILIKTRNYPVAINNLAKSEVYLEKIKNQSLKEVEKFDLFISLAMYYAKSNEK
                     SFEQSICYLDRAKNILNMIKNIEEYTIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLE
                     KSKNAKNYNYVKLAYKNIYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLN
                     YRHEYIETEINNMRKVKLDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDV
                     DYFKEYNDTYGHYNGDIALKNITIIIKKYLKKDMFMIRYGGEEFLILSVCKDYKKSKI
                     FGRKLCRIVKKFLNNNLTVSIGIDTCKNSSIDIREIIENADKALYKAKESGRNRCLHY
                     HDFKNF"
     gene            1224..3110
                     /locus_tag="KR440_003229"
     CDS             1224..3110
                     /locus_tag="KR440_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBH0700235.1"
                     /translation="MKRSLIEDLKRNINKIEYDMLTKFYLNLTDLNYILDYHKDLLNT
                     KLEDNSRVYTLYLISLINYIKKDREASYNYLIESLKYIKKLSLKEDSDIISKIYIYLT
                     LSAISIRKYDKANNFYFSAKKVIRQNHLNELLVLLNMSLCVEISALYQTKTDVIALVE
                     EVSFFKTLKNKALVSRANYIFGRVYLYLFNNFIKSMEYLIECLKLAQENNLYSIEAFC
                     KCIISLCYLESGNNVEAIKYINNVLDVAHSKNSISVTERVSIKIDLIWAYLKEDMNKE
                     AEVILLKTIKLMKVVEGVYKDYLYSFIFLYSAEIELRKDNCSFEKVNTYLILSKNIYK
                     SFGDVFIYNSKLDYIYKLYGDLYIKFGNIDEGISCYIKGLNLVKKSSLNLRKISIFYG
                     LIANGYELKKDFKLAIEYYKSMDWYSDRWEKNNSYKTSQAIHKQYELRQKQESLRLLV
                     DDNKKLENDILKDGLTKLYNRRYLEQQLDLFNGTRDKNMVAILVDIDCFKSYNDNYGH
                     LAGDKVIVKVTDIIRSVFIDITEHIIRYGGEEILILVDSIESIGVEEIDNIELYIKRV
                     FKLLEFEGIEHIYSKIKNYITISAGVSIKRCRSRKDVEILIEDADKNLYKSKKAGRNQ
                     YIII"
     gene            3118..5118
                     /locus_tag="KR440_003230"
     CDS             3118..5118
                     /locus_tag="KR440_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0700236.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEVVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTREFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
BASE COUNT         2263 a          428 c          888 g         1693 t
ORIGIN      
        1 tcactatgta atataaagat aggtgagtgt gaatgttcat ttgaaaatta tgaatgttcc
       61 atagaatatt taaatgaagt aataagcaat aaaaagtata gaaatgtaaa tataattcaa
      121 aaatatagag catcaaacaa cataactaaa atattaataa agactagaaa ttatcctgta
      181 gcaataaata atttagcaaa atctgaagta tatctagaaa aaattaaaaa tcagagctta
      241 aaagaagtag agaagtttga tttatttata agtttagcta tgtattatgc aaaaagtaat
      301 gaaaaatctt ttgaacaatc aatatgctat ttagatagag ctaaaaacat cttaaatatg
      361 ataaaaaata tagaagaata tacaatatat gatttagaaa taatttatca tcaggcaaag
      421 atatattata tctttggata ttatgacaat gccttaaaca caaataaaaa actccttgaa
      481 aaatctaaaa atgctaaaaa ttataattat gtgaagttag catacaagaa tatatatttg
      541 tgttttgaaa agattcaaaa ttatgaaatg agtatgaaat actttaaaat gtattatgag
      601 ttaaaaatga tgtatataaa agctcgaaat agaaattata tagattcctt aaattataga
      661 catgagtata tagaaacaga aataaataac atgagaaaag taaaacttga tttagataaa
      721 aaaaaatatg ttgaccattt gacaagtgca tataatagaa cttttttgaa cgatttttta
      781 gaaaaacaag aagttagtga atattgcaca gcatttatga tagatgtaga ttactttaaa
      841 gaatacaatg atacttatgg acactataat ggagatattg ctcttaaaaa tataactata
      901 ataataaaaa aatatctaaa aaaagacatg tttatgataa ggtatggtgg agaagaattt
      961 ctgatattat ctgtatgtaa agattataaa aaaagcaaaa tatttggaag aaaattatgt
     1021 aggattgtaa agaaattttt aaacaataat ttgacagtta gtattggcat tgatacatgt
     1081 aaaaatagta gtatagatat aagagaaata attgaaaatg ctgataaagc tttatataaa
     1141 gcaaaggaaa gtggtagaaa tagatgtttg cattatcatg attttaagaa tttttaactg
     1201 ttgaaatagg attggtgatt tacttgaaaa ggagtttaat agaagattta aagagaaata
     1261 taaacaagat agaatacgat atgttgacta aattctatct aaatttgaca gatttaaact
     1321 acatcttgga ttatcacaaa gaccttttaa atacaaaact agaagataat agtagagtct
     1381 atactttata tttaattagc cttataaact atataaaaaa agatagagaa gcatcataca
     1441 actatttgat agagtcatta aagtatatta aaaaattgtc attaaaagaa gattcagata
     1501 taatatctaa aatttatatt tatcttactt tatcagctat ttctataaga aaatatgata
     1561 aggcaaataa tttttatttc tcagcaaaaa aagttataag acaaaatcat ttaaatgagt
     1621 tgctagtctt gttaaatatg agtttatgtg tagaaataag tgctttatat cagaccaaaa
     1681 cggatgtaat agctttagta gaagaagtgt ctttttttaa aactttaaaa aataaagctc
     1741 ttgtaagtag ggcaaattat atatttggga gagtttatct atatttattt aataacttta
     1801 taaaaagtat ggagtaccta attgaatgtc taaaactggc tcaagaaaat aatttatatt
     1861 ctatagaggc tttttgtaaa tgtataataa gtttgtgtta tttagagagt ggaaataatg
     1921 tagaagctat aaaatacata aataatgtat tagatgttgc gcacagtaag aactcaatat
     1981 cagtaactga aagagtgtct ataaagatag accttatatg ggcatattta aaagaagata
     2041 tgaataaaga agcagaagtt attttattaa agactataaa attaatgaaa gttgttgaag
     2101 gtgtatataa ggattattta tattcattta tatttttata ttcagctgaa attgagttaa
     2161 ggaaagataa ttgtagtttt gaaaaggtaa atacctattt aattttatca aaaaatatat
     2221 ataaaagttt tggcgatgtt tttatatata attctaaact agattacata tataagttgt
     2281 atggagattt atatataaaa tttggtaata tagatgaagg tataagttgt tatataaaag
     2341 gtttaaattt agttaaaaag agttcattga atcttagaaa aatatctata ttttatgggc
     2401 taatagcaaa tggatatgag ctaaaaaaag attttaaact ggcaatagaa tattataaaa
     2461 gtatggattg gtattctgat aggtgggaaa aaaataattc ctataaaaca tctcaagcaa
     2521 ttcacaaaca atatgaactc agacaaaaac aagagagttt aagattacta gtggatgata
     2581 ataagaaatt agaaaatgat attttaaaag atggacttac taaactatat aatagaagat
     2641 atttggaaca gcagttggat ttatttaatg gaaccagaga caaaaatatg gttgcaatac
     2701 tagtcgatat agattgtttt aaaagttata atgataatta tggtcatcta gcaggagata
     2761 aagttatagt taaggtaact gatattataa gaagtgtatt tatagatatt accgaacata
     2821 taatcagata tgggggagaa gaaattttaa tactagtaga ttctatagaa tcaatcggtg
     2881 ttgaagagat tgataatata gaattatata ttaaaagagt tttcaaactt cttgaatttg
     2941 agggtattga acatatatat tctaaaataa aaaactatat aactataagt gctggagtaa
     3001 gtataaaaag atgtcgtagt agaaaagatg ttgaaatatt aattgaagat gcagataaga
     3061 acctatataa atcaaaaaaa gcaggtagaa atcaatacat tataatttaa aattgaggtg
     3121 attttgttgc gaaaaaaaat aggaattata gcctctgata ttgaattaaa agaaagaata
     3181 gaagaattat atagagaaga tgtagaaaat ggaactataa taatcgacat acttaaccta
     3241 gacttaatgg aaaatcaagg tcgtatactt gttgaaaaag gtgcccaagc tataattggt
     3301 agaggtggag gttatagttt agtaatagat acagtaaatg tacctgttat accaatgaat
     3361 atgaagtcaa ctgacctttt aagagcaata gaaatagcaa agaaatattc taaaaaagta
     3421 gtattgattc ttggagacaa tgaagtttct tttgactatg ttggctggag aaatgtcata
     3481 agtactgaaa taactgaaga atggtttgaa tctaagtatg agataagaag taaagttgta
     3541 aaatatatag accaaaaaga tgaagttgta atcgtaggag gaggtttggc atgttctttt
     3601 gcaaggcaat atggaattga tagtgtattt gcaactgcta gtgatgaatc tataagagaa
     3661 gctgtagaat attgtaaaaa attattggat acattaggtg aagaaaagtt taataatgaa
     3721 gtgttacgaa atatacttga tggtataaaa gatggagtaa ttgctataga tagtaatggt
     3781 agtataattt tgtacaatga aagtgcaaaa aacatgctta aagtagagag aaaatgtgca
     3841 cttaataaat acatcttaga tgtatttcct aaaatggagt ggatgttaga ttgtttacat
     3901 gaaaaagagg atgtagaaga tagaaagata agaaatataa ataatctaat tgttaatact
     3961 aggacaacat taataaaagt tgataatagt acttatggag ttttaggaat aatacaagat
     4021 ataaccaagc tacaaaactt agaaagaaaa ataagatttg acttgaatca aaaaggtctc
     4081 tatgctagat atacttttga tgatttttta tttaaagata aattaactag agaatttatt
     4141 gaagaagcta aaaaaattgg aaaaagtgat tatacaactt tactttatgg agaaagtggg
     4201 tctggtaaag aaattatagc tcatagtata cacaatataa gtaaaagaaa agataggcct
     4261 tttgttgcta taaattgtgc tactatagca gaaaatttac ttgaaagtga actttttggc
     4321 tatgaagaag gtgcatttac tggtgctaga aagggtggaa aaagaggact atttgaactt
     4381 gctcatggag ggactctttt tttagatgaa ataaatagtc tatcctttaa tattcagaca
     4441 aaactattaa gagtaataga ggaacgtcaa ataatgagaa ttggttctga ttatataata
     4501 cctctggata taaggattat agcagcaacg aacgaatctt taaccgaaaa aatagttatg
     4561 ggtacattta gagcagactt attttataga ttgagtagtt tagagataaa tatacctcct
     4621 ttaagagaca gaagagaaga tataatacct ttgtttaaca attttgtaaa tgaagtatta
     4681 aaggatgatg gtctcaatgg aataaacagt atagatgaaa attttgtttt gactaaagat
     4741 gaaatggata agctatataa ttatagctgg cctggaaatg taagagagtt aaagacaata
     4801 gctcaaaaat atgttgtgac aggtaaaatt aaattgagac aagatagaga ttttaaaact
     4861 aaaaaattac ttcctaactc tgaagtagac aaatttaatt ctgaaacgac agcaagtgta
     4921 gaagtccaag atgaatctat aaatataagt aagataaatg atggtaagat aagtatagat
     4981 ataaaagaag taaataagta tgttgaagaa aaaataataa gtatgctttt tgctcaggga
     5041 ctttctaaaa atgaagttgc acaagttctt ggaataagca ggacatcttt atggaagaaa
     5101 tacaataaaa atatttaaat ataattacat ataaattatt ttgaagtaat gttaattttt
     5161 taaaacaagt gttaagaaaa attaacatta cttttttgta aatttttatt tttttagaca
     5221 ggcatatata tttataagaa aaatagttaa aacacttaat ttaataatga aa
//
LOCUS       DAEPOZ010000245         5190 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.245, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000245 DAEPOZ010000000
VERSION     DAEPOZ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.245"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..201
                     /gene="tcdR"
                     /locus_tag="KR440_003231"
     CDS             <1..201
                     /gene="tcdR"
                     /locus_tag="KR440_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HBH0700237.1"
                     /translation="EDICFFEYILKELSGIQRKVIFYKYLKGYSDREISVKLKISRQA
                     VNKAKNRAFKKIKKDYENYFNL"
     gene            532..>5190
                     /gene="tcdB"
                     /locus_tag="KR440_003232"
                     /gene_synonym="toxB"
     CDS             532..>5190
                     /gene="tcdB"
                     /locus_tag="KR440_003232"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HBH0700238.1"
                     /translation="MSLVNRKQLEKMANVRFRVQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTIVESATNDTLESFREN
                     LNDPRFDYNKFYRKRMEIIYDKQKNFINYYKTQREENPDLIIDDIVKIYLSNEYSKDI
                     DELNSYIEESLNKVTENSGNDVRNFEEFKGGESFKLYEQELVERWNLAAASDILRISA
                     LKEVGGVYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMVKLEAIMKYKEYIPGYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNAFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKKNYFEGSLGEDDNLDFSQNTVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGLDVDSLSTEIETAIDLA
                     KEDISPKSIEINLLGCNMFSYSVNVEETYPGKLLLRVKDKVSELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKIIVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLAECEINVISNIDTQVVEGRIEEAKSLTSDSINYIK
                     NEFKLIESISDALYDLKQQNELEESHFISFEDILETDEGFSIRFIDKETGESIFVETE
                     KAIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDATHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     VIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTAATTAIITSSLGIASG
                     FSILLVPLAGISAGIPSLVNNELILRDKATKVVDYFSHISLAESEGAFTSLDDKIMMP
                     QDDLVISEIDFNNNSITLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPVITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMNINIELNENDTWVIDVDNVVRDVTIESDKIKKGDLIENILSKLSIE
                     DNKIILDNHEINFSGTLNGGNGFVSLTFSILEGINAVIEVDLLSKSYKVLISGELKTL
                     MANSNSVQQKIDYIGLNSELQKNIPYSFMDDKGKENGFINCSTKEGLFVSELSDVVLI
                     SKVYMDNSKPLFGYCSNDLKDVKVITKDDVIILTGYYLKDDIKISLSFTIQDENTIKL
                     N"
BASE COUNT         2108 a          543 c          863 g         1676 t
ORIGIN      
        1 gaagatattt gttttttcga atatattttg aaagaattat ctggtattca aagaaaagtt
       61 attttttata aatatttaaa aggatattct gatagagaaa tatcagtgaa attaaaaata
      121 tctagacaag ctgttaataa ggctaaaaat agagcattta aaaaaataaa aaaagactat
      181 gaaaattatt ttaacttgta attaatgaat ttaaagaaat atttacaata gaaatcaaat
      241 tttagaatta actttattgt aaaatcaata acttaatcta agaatatctt aatttttata
      301 ttttatatag aacaaagttt acatatttat ttcagacaac gtctttattc aatcgaagag
      361 caaattaatc aactgagtgt cttcaattta aaatgttagg aagtgaatgt atatgaaaac
      421 ctaagtagat attagtatat tttataaata gaaaggagga tatataaaag agttttagca
      481 tttagatgta aaaatattca ataaaaatat tatagtaaag gagaaaattt tatgagttta
      541 gttaatagaa aacagttaga aaaaatggca aatgtaagat ttcgtgttca ggaagatgaa
      601 tatgtagcaa tattggatgc tttagaagaa tatcataata tgtcagagaa tactgtagtc
      661 gaaaaatatt taaaattaaa agatataaat agtttaacag atatttatat agatacatat
      721 aaaaagtctg gtagaaataa agccttaaaa aaatttaagg aatacctagt tacagaagta
      781 ttagagctaa agaataataa tttaactcca gttgagaaaa atttacattt tgtttggatt
      841 ggaggtcaaa taaatgacac tgctattaat tatataaatc aatggaaaga tgtaaatagt
      901 gattataatg ttaatgtttt ttatgatagt aatgcatttt tgataaacac attgaaaaaa
      961 actatagtag aatcagcaac aaatgataca cttgaatcat ttagagaaaa cttaaatgac
     1021 cctagatttg actataataa attttacaga aaacgtatgg agataatcta tgataagcag
     1081 aaaaatttca taaattacta taaaactcaa agagaagaaa atcctgacct tataattgat
     1141 gatattgtaa agatatatct ttcaaacgag tattcaaagg atatagatga acttaattcc
     1201 tatattgaag agtcattaaa taaagttaca gaaaatagtg ggaatgatgt tagaaacttt
     1261 gaagaattta aaggtggaga gtcattcaaa ttatatgaac aagagttggt agaaagatgg
     1321 aatttggcag ctgcttctga catattaaga atatctgcct taaaagaagt tggtggtgta
     1381 tatttagatg ttgatatgtt accaggaata caaccagacc tatttgagtc tatagagaaa
     1441 cctagttcag taacagtgga tttttgggaa atggtaaaat tagaagctat aatgaaatac
     1501 aaagaataca taccaggata tacttcagag cattttgata tgttggatga agaagttcaa
     1561 agtagttttg aatctgttct agcttctaag tcagataagt cagaaatatt ctcatcactt
     1621 ggcgatatgg aggcatcacc actagaagtt aaaattgcat ttaatagtaa aggtattata
     1681 aatcaaggac taatttctgt gaaagactca tattgtagca atttaatagt aaaacaaatc
     1741 gagaacagat ataagatatt gaataatagt ttaaatccag ctattagcga ggataacgat
     1801 ttcaatacta caacgaatgc ttttattgat agtataatgg ctgaagctaa tgcagataat
     1861 ggtagattta tgatggaact aggaaagtat ttaagagttg gtttcttccc agatgttaaa
     1921 actactatta acttaagtgg ccctgaagca tatgcggcag cttatcaaga tttattaatg
     1981 tttaaagaag gtagtatgaa tatccatttg atagaagctg acttaagaaa ctttgaaatc
     2041 tctaaaacta atatttctca atcaactgaa caagaaatgg ctagcttatg gtcatttgac
     2101 gatgcaagag ctaaagctca atttgaagaa tacaaaaaaa attactttga aggttctctt
     2161 ggagaagatg acaatcttga cttttctcaa aatacagtag ttgacaagga gtatctttta
     2221 gaaaaaatat cttcattagc aagaagttca gagagaggat atatacacta tattgttcag
     2281 ttacaaggag ataaaattag ttatgaagca gcatgtaact tatttgcaaa gactccttat
     2341 gatagtgtac tgtttcagaa aaatatagaa gattcagaaa ttgcatatta ttataatcct
     2401 ggagatggtg aaatacaaga aatagacaag tataaaattc caagtataat ttctgataga
     2461 cctaagatta aattaacatt tattggtcat ggtaaagatg aatttaatac tgatatattt
     2521 gcaggtcttg atgtagattc attatccaca gaaatagaaa cagcaataga tttagctaaa
     2581 gaggatattt ctcctaagtc aatagaaata aacttactgg gatgtaacat gtttagctat
     2641 tctgtaaatg tagaggagac ttatcctggg aaattattac ttagagttaa agataaagta
     2701 tcagaattaa tgccatctat aagtcaagac tctattatag taagtgcaaa tcaatatgaa
     2761 gttagaataa atagtgaagg aagaagagaa ttattggatc attctggtga atggataaat
     2821 aaagaagaaa gtattataaa ggatatttca tcaaaagaat atatatcatt taatcctaaa
     2881 gaaaataaaa ttatagtaaa atctaaaaat ttacctgaat tatctacatt attacaagaa
     2941 attagaaaca attctaattc aagtgatatt gaactagaag aaaaagtaat gttagcagaa
     3001 tgtgagataa atgttatttc aaatatagat acacaagtgg tagaaggaag gattgaagaa
     3061 gctaaaagct taacttctga ctctattaat tatataaaga atgaatttaa actaatagaa
     3121 tctatttctg atgcactata cgatttaaaa caacagaatg aattagaaga gtctcatttt
     3181 atatcttttg aggatatatt ggagactgat gaaggcttta gtataagatt tattgataaa
     3241 gaaactggag aatctatatt tgtagaaact gaaaaggcaa tattctctga atatgctaat
     3301 catataactg aagagatttc taagataaaa ggtactatat ttgatactgt aaatggtaag
     3361 ttagtaaaaa aagtaaattt agatgctaca catgaagtga atactttaaa tgctgcattt
     3421 tttatacaat cattaataga atataatagt tctaaagaat ctcttagtaa tttaagtgta
     3481 gcaatgaaag ttcaagttta tgctcaatta tttagtactg gtttaaatac tattacagat
     3541 gcagccaaag ttgttgaatt agtatcaact gcattagatg aaactataga tttacttcct
     3601 acattatctg aaggattacc tgtaattgca actatcatag atggtgtaag tttaggtgca
     3661 gcaatcaaag agctaagtga aacaagtgac ccattattaa gacaagaaat agaagctaag
     3721 ataggtataa tggcagtaaa tttaacagca gctacaactg caatcattac ttcatcttta
     3781 ggaatagcta gtggatttag tatactttta gttcctttag caggaatttc agcaggtata
     3841 ccaagtttag taaacaatga gcttatccta agagataaag caacaaaagt tgtagattat
     3901 tttagtcata tatcattagc tgagtctgaa ggagcattta cttcattaga tgataaaata
     3961 atgatgccac aagatgattt agtaatatct gagatagact ttaataacaa ttcaataact
     4021 ttaggtaaat gtgaaatctg gagaatggaa ggtggctcag gtcatactgt aactgatgat
     4081 atagatcact tcttttcagc accatcaata acatatagag agccacactt atctatatat
     4141 gacgtattgg aagtacaaaa agaagaactt gatttgtcaa aagatttaat ggtattacct
     4201 aatgctccaa atagagtatt tgcttgggaa acaggatgga caccaggttt aagaagctta
     4261 gaaaatgatg gcacaaaact gttagaccgt ataagagata actatgaagg tgagttttat
     4321 tggagatatt ttgcttttat agctgatgct ttaataacaa cattaaaacc aagatatgaa
     4381 gatactaata taagaataaa tttagatagt aatactagaa gctttatagt cccagtgata
     4441 actacagaat atataagaga gaaattatca tattcttttt atggttctgg aggaacttat
     4501 gcattatctc tttctcaata caatatgaat ataaacatag aattaaatga aaatgatact
     4561 tgggttatag atgtcgacaa tgtcgtaaga gatgtcacta tagaatctga taaaattaaa
     4621 aaaggagatt taatagagaa tattttatct aaattaagta ttgaagacaa taaaattatt
     4681 ttagataatc atgaaattaa tttctctgga acattaaatg gaggtaatgg atttgtatct
     4741 ttaacattct caatcttaga aggaataaat gcagttatag aagttgattt attatctaaa
     4801 tcatataaag ttcttatttc tggtgaacta aaaacattga tggcaaattc aaattctgtt
     4861 caacagaaaa tagattatat aggattgaac agcgaattac aaaaaaatat accttatagt
     4921 tttatggatg ataaaggaaa agaaaatgga tttattaatt gttctacaaa agaaggttta
     4981 tttgtatctg aattatctga tgtagttctt ataagtaaag tttatatgga caatagtaaa
     5041 cctctatttg gatattgtag taatgatttg aaagatgtta aagtcataac taaagatgac
     5101 gttattatat taacaggata ttatttaaaa gatgatataa aaatctctct ttcttttact
     5161 atacaagatg aaaatactat aaaattaaat
//
LOCUS       DAEPOZ010000246         4954 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.246, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000246 DAEPOZ010000000
VERSION     DAEPOZ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4954
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.246"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            207..1100
                     /locus_tag="KR440_003233"
     CDS             207..1100
                     /locus_tag="KR440_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBH0700239.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIAEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYNNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEV"
     gene            1148..3448
                     /locus_tag="KR440_003234"
     CDS             1148..3448
                     /locus_tag="KR440_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700240.1"
                     /translation="MKKIFSIFISCMVVVTTITGCSSGEVIKNTSPINSVKNLDFSFE
                     VKPEIFEVSVESGGSKEKISEPSKERKVSNFKKNKTEASWTYPDENIDINIKKEDSYL
                     DVNIKSNLEKETSFNWPKVEGESYILPLYEGKFIPSNDKYWKEYLNESTYNVIESFSM
                     QFFAVNKAKYSVIYIIENEFNNVIEFSSKDKINFTFKHEYPSINKNKEYGFKIYLTNK
                     NITDVAKTYKNYIVDKGEFKTLKEKSKENKNIEKLYGAPHIYFGDKSIITINDIEWNK
                     LKENLSDDLTNWMKELLSMKSGNSEFIKVINQIKTQDYIDKYQKNQIVQAFNEVMQMN
                     TFYNEKVFESKDREIKKVLDKGISKLSRVELIEFNKKLLKSKLEESVNDVATWAKYNT
                     TDVINDINKSGIKNAWIGFDDMEEGYISLEMVKRANELGYLVGPYDSYHSIHKPGEEK
                     WSTASFKDKSLYENATIENKKGEKETGFQGEGRKLNPTLSLPSVKERVQSILDEGYEF
                     NSWFIDCDATGEIYDDYSQNHITTQTQDLNARLERMSYIRDHANMVIGSEGGNDFSSS
                     TIAFAHGIETPAFAWVDKDMSKNKGSEYYVGKYYSPTGGVPEIFSKQIPVKERYKKIF
                     IDPIYSLPLFRLVYNDSVISSHQWLWGTLKIKDEVGSRMMKEVLYNTPPMYYIDRTEW
                     EKHKDEIISNNKVWSAFNKKAIKKEMTDFKFISEDRLVQMTEYGEDLKVLVNFSQKDI
                     KYNDEVIKANSLVIYDADSKVIYKPE"
     gene            3682..4173
                     /locus_tag="KR440_003235"
     CDS             3682..4173
                     /locus_tag="KR440_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HBH0700241.1"
                     /translation="MYENNKVIYNFERCEKLENALYLSQFNTYKNFKLTSKKTVINSL
                     DTSTYNMNIHSISLVDTMECISSEDEQMTGKKLILVGDIDTDVTLDYAGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSS
                     KIE"
     gene            4201..>4954
                     /locus_tag="KR440_003236"
     CDS             4201..>4954
                     /locus_tag="KR440_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700242.1"
                     /translation="MRITKTTFNDVFSNDANGSIIKVEKYVDKKCASVFEILTYTIIV
                     TNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNSIVFGCIEVEIDYSCKNR
                     SNFKTVNKFNDIKKDKYYKNDIQNNNENINDCK"
BASE COUNT         2124 a          451 c          813 g         1566 t
ORIGIN      
        1 atataaatag ttaaaataag tttatttaat tatttatcta ttttaactat tttgtgtgat
       61 agaaaactat atattataac aatagtagtg cctttaatta aatatatttc ataaatcttc
      121 ataaaatctt caaaatttcg taaagaatat gtcacaaaag taaggtatat tatattcagc
      181 agatagtata agggaggaca atatttatga agaataaaaa attcattgtt ataatactga
      241 taatatcaat tttaataggt gtattagtga aggaatattc atctagagag ataaaaaagg
      301 atgatataaa tgtcagtaag tatataaaat atgctgattt agcaagtaaa aataatgcac
      361 aagttaactg gaagtatgta gcttccattg ttgctgtgtt aaataaaaat aacttaaaaa
      421 acgttaagga ttcacagata caagaagtat cagatttatt tgttaagaat ttttctaaaa
      481 ataataaaat aaataagtta agtgatattt tggatgagtt agaatttagt aatagacaaa
      541 aaagattggt tgataattat atagacaatc ttaaggatta tggtataaaa ccagaaagat
      601 tgaagtctga tacaaaatat atgaagttta ttgctgaaat taaaacagaa gcaatacaaa
      661 attataagga ttataaaata cttccatcta taactattgc acaagctata atagaatcta
      721 gctggggcaa gtcaactttg gcaaaacaat ataacaatct atttggtata aaggctgacg
      781 cttattggaa aggtaagtca gtgactttag aaacgaaaga acatttagat acaattatag
      841 atgataaatt tagaatatat gatgataaaa atgaatctat aaaagaccat gcaaaatttt
      901 tagccacaaa taaaaggtat aaaaacaatg gtgtatttga tgcaaaaacg tatatatatc
      961 aagctaaagc attagaaaag gcaggttata gtactgcgaa ggatgaaaat ggcaatagta
     1021 tatatgcagc tcgtctaata gaattaattc aacagtataa tcttcaatta atagacagcg
     1081 aaattcaaag tgaagtgtaa cttttatgta acataggagt gatatattta tcgaggaggt
     1141 ggtttttatg aaaaaaatat tctcgatatt tataagttgt atggttgttg taactactat
     1201 tactggatgt agtagtggtg aggtgataaa aaatacatca cctatcaata gtgttaaaaa
     1261 tttggatttt agttttgagg tgaaacctga aatatttgag gtatcagttg aatctggtgg
     1321 ttcaaaagaa aagatatctg aaccctcaaa agaaaggaaa gtatcaaatt ttaaaaagaa
     1381 taagacagag gcatcgtgga cgtatcctga tgaaaatata gacataaata taaaaaaaga
     1441 ggacagctat ttagatgtaa atattaaatc taatttagaa aaagaaacta gttttaattg
     1501 gcccaaagtt gaaggggaaa gttatatact accactttat gagggtaagt ttataccaag
     1561 taatgataag tattggaagg agtatctaaa tgagtcaact tataatgtta tagaatcttt
     1621 ttcaatgcaa ttctttgcag taaacaaggc aaagtattct gtgatttata ttatcgaaaa
     1681 tgaatttaac aatgtaattg aatttagttc taaagacaag attaatttta catttaaaca
     1741 tgaatatcca agtatcaata aaaataagga gtatgggttt aaaatatatt tgactaataa
     1801 gaatattaca gatgtagcaa aaacttataa aaattatata gttgataaag gagaatttaa
     1861 aacattaaaa gaaaaatcta aggaaaataa aaatattgaa aaactttatg gagcaccaca
     1921 tatttatttt ggagataagt ctatcatcac aataaatgat attgaatgga ataaattaaa
     1981 agaaaattta tcagatgatt tgactaattg gatgaaagag cttttaagta tgaagtctgg
     2041 caatagtgaa tttataaaag taattaatca aataaaaact caagattata tagataaata
     2101 tcaaaaaaat caaatagtac aagcatttaa tgaagttatg cagatgaata ctttttataa
     2161 tgaaaaagtt tttgaaagca aagatagaga aataaaaaaa gtattagata aaggtatatc
     2221 taaattaagt agggtagaac ttatagagtt taataagaaa ttattgaaat caaaattgga
     2281 agagagcgtg aatgatgttg ctacatgggc aaaatataat acaactgatg taattaatga
     2341 tattaataag tctggtatta aaaatgcgtg gataggattt gatgatatgg aggaaggtta
     2401 tatatcctta gagatggtta aaagagctaa tgagttaggt tatctcgtag ggccatatga
     2461 ttcataccac tctatacaca aaccagggga agaaaagtgg agtacagcat catttaaaga
     2521 taaatctcta tatgaaaatg ctaccataga aaataaaaaa ggtgaaaaag aaactggatt
     2581 tcaaggagaa ggaagaaaac taaatcctac attgtcttta ccaagtgtaa aagagagagt
     2641 acaaagtata ttggatgaag gatatgaatt taattcttgg tttatagatt gtgatgctac
     2701 aggagaaatc tatgatgatt attctcaaaa tcatataaca acacaaacac aagatttaaa
     2761 tgctagactt gaaagaatgt catatataag agaccatgca aatatggtca taggttcaga
     2821 aggtggaaat gatttttcaa gtagtactat agcatttgca catggtattg agacaccagc
     2881 atttgcttgg gtagataaag atatgagtaa gaataaaggc agtgaatatt atgttggtaa
     2941 atactattct ccaacaggag gagtaccaga aatattttca aagcagatac ctgtaaaaga
     3001 aaggtacaaa aaaatattta tagacccaat ttactctcta cctttattta gattagttta
     3061 taatgattct gtgatatctt cccatcagtg gttatgggga actttgaaga ttaaggatga
     3121 agttggaagt agaatgatga aagaagtatt atataataca ccacctatgt attatataga
     3181 tagaactgaa tgggaaaaac ataaagatga aataatctca aacaataaag tatggtcagc
     3241 gtttaataag aaagctatta aaaaagaaat gacagacttt aaatttattt ctgaggatag
     3301 actagttcaa atgactgaat atggagaaga tttaaaggta ttagttaatt tttctcaaaa
     3361 agacattaag tacaatgatg aagtcataaa agcaaattct ttggtaatat atgatgctga
     3421 tagtaaagtc atatataaac cggaataata gatttaatga aaataaagat tgtttaaatt
     3481 tcaagtaaaa aataatataa tattcattta gtttgtatta aagtagtgac tatattaatc
     3541 tggataatta gtatagtcac tattatttat attctttcct aaaagtaagt agtaactgaa
     3601 tcgtgaataa tcaaaaatgg taatttgaaa tagtttttat gaatagtatt aatatataga
     3661 tatagggaga ttgatgtgat tatgtatgaa aataataagg taatatataa ttttgaaaga
     3721 tgtgaaaagc tagaaaatgc tctttattta tctcaattta atacatataa aaactttaag
     3781 ttaacttcta aaaaaactgt aataaactca ttagatacat ctacttataa tatgaatatt
     3841 catagcataa gtctcgttga tactatggaa tgtatatcgt cagaagatga acaaatgaca
     3901 ggaaagaagt tgattttagt tggcgatata gatactgatg taacattaga ttatgcaggc
     3961 aataaaagaa atagaaattc tagtaaaaaa aatacattta aattaaaaat accctttagt
     4021 acatttatac aaatgcctag aaagatagaa aataaagata aaattaattt aaagtatctt
     4081 atacaagata ttacttcatc tattttagat gataatttat ttattagtgt tactgcaatt
     4141 ataagttatg aaaatagttc caagatagag taatctttaa attatagaaa ggtggatgca
     4201 atgagaataa cgaaaactac ttttaatgat gtattttcaa atgatgcaaa tggaagtatt
     4261 ataaaagtag aaaaatatgt tgataaaaaa tgtgcatctg tatttgagat tttgacctac
     4321 actattattg ttacaaatac aagtaggtac aaaactggaa atatattttt caaagattat
     4381 atttctaaat atatagagtt tataaataat actgtaaaag ttaatggcat tataaaacgt
     4441 ggattagacc cacagcaagg tttttacata gggagaatag atgcaagttg taaaaaaata
     4501 atatctttta agagtgtagt tttaccaaat tctgcacata ggattataaa gaatagtgct
     4561 aatatatact attattataa gtgtaatcta gataaattcc caacaagaat atcaatagaa
     4621 agtaatagag tttatactaa tgtaaataaa attgtattca aacaattgaa tataagtaat
     4681 actttaaaag ctcctaaaaa tctaagagat atattaaaag taaatgtaaa tagtaaaata
     4741 tttgatgtta aacctatgaa aaatcttata aacaaaggta gtaatttgaa attatgtaac
     4801 tcgatagttt ttgggtgtat agaagttgaa atagactata gttgtaaaaa taggagtaat
     4861 tttaaaacag taaataagtt taatgatatt aaaaaagata agtattataa aaatgatata
     4921 caaaataaca atgaaaatat aaacgattgt aaaa
//
LOCUS       DAEPOZ010000247         4940 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.247, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000247 DAEPOZ010000000
VERSION     DAEPOZ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.247"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            8..679
                     /locus_tag="KR440_003237"
     CDS             8..679
                     /locus_tag="KR440_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0700243.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKACLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            681..1589
                     /locus_tag="KR440_003238"
     CDS             681..1589
                     /locus_tag="KR440_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0700244.1"
                     /translation="MFILAFIICTVVIFIVSTKINQRRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKSEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            1666..2352
                     /locus_tag="KR440_003239"
     CDS             1666..2352
                     /locus_tag="KR440_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0700245.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGEVL"
     gene            2349..4904
                     /locus_tag="KR440_003240"
     CDS             2349..4904
                     /locus_tag="KR440_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBH0700246.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKKNKKSKEYIVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETAMEGFVNDYLQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIGVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKRGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTGEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKS"
BASE COUNT         2011 a          509 c          826 g         1594 t
ORIGIN      
        1 agtaaaattg aacttattaa ttattgaaga tgatataaac ttaaatgaag gtctttttta
       61 tgcatttgag aatgatggat ttaatgtatt taaggcttat acaaaacaag aaggattaaa
      121 tatatttaat agtaaaaata tagattttat aatcttagac tgcaatcttc cagatggaga
      181 tggatttgat gtatgtcaaa taataaggga aaagtcagat ataccaatta taatgcttac
      241 tgcaagagac tcagaaattg atgaagtaaa aggattagaa atagggcttg atgattatat
      301 tacaaaacca ttttctttat ctgtattaaa ggcaagagtc aaagtagcaa taagaaaaaa
      361 atcgaataat aaagtaatat attccaatgg aataaaattg gaccaaaagt tactaaaagt
      421 atataaaaat aaagcatgtt tggaacttag ctctgtagaa tataagcttc taagttatct
      481 aattgaaaat aaaggtcaga tattattaaa ggaacaaatt ttacaccata tttgggacag
      541 cgaagaaaat tatgttgatg ataatattgt atctgtaaat ataagaagac ttagagtaaa
      601 agtagaggat gacccatcta atccaaaata tataaaaaca gcttatggaa tgggttattt
      661 atggaatgag gtagaataat atgtttatat tagcctttat aatatgtaca gttgttatat
      721 ttatagtatc tactaaaata aatcaaagaa gatatgatga attaaatttg atgttaaatc
      781 aaatattgga aggtaaagaa gttacttatc cagatacgaa ggatacaaga gcgtcaaaaa
      841 tttctcacca agtaaaaaaa attaaggata tgatagaaat agaggtagaa cagtcaaagt
      901 cagaaaaaga agcgattaaa ggtttaatat caaatatgtc acatcaatta aaaactccac
      961 tttcaaatat aaccatttat tgtgaacttc tggagaatat aaatatttcc acattacaga
     1021 agaaggagtt tttacaaaag atgaaaaatg aaacttttaa gattgactgg ttgttacaat
     1081 ccttatttaa aatgactaaa ttagaggatg gagttataga atttgaagta gaagaactac
     1141 taattaaaga tactcttatt caaagcatca gtactatctt taataaatca gaagctaaaa
     1201 atataaggat aaatctcgaa ccattttcag atataaagtt agttcataat aaaaagtgga
     1261 ccatagaagc aattgtaaat gttttagaaa atgcaataaa atactcacca tcagatagca
     1321 cgattactat ttctgtaata aaaatggagc tatatacgaa gattactatt aaagatgaag
     1381 gaataggtat tgatagtaga gaactaaatg atatttttaa aagattttac agaagtaaaa
     1441 atgtggaaaa ccaaaatggc acagggattg gtctatatct tacaagatta attttagaaa
     1501 aagaaaatgg aaacattata gtagaatcta aactaggaag tggatgttgt tttagtatat
     1561 tcttacagaa ttgtaagagt ttaaactaac tttgtaggat gagtgtaatt atcgtcactt
     1621 acaatatttt atagaacata tatctgtatt gaaaggagaa attaaatgag tatattagtg
     1681 acgaataatt tagtgaaaca ctatggaaaa ggtgagacta aagtaaaagc cttaaatgga
     1741 gtaagtatag aaattgaacg agatacattt actgctatag ttggaacttc tggttctgga
     1801 aaaagcacat tgttaaatat tataggtgga ttagataatc caacctcagg tgatgtaata
     1861 attaaaggaa agaacatatc taaaatagga aaaaaagatt tgacagtatt tagaagaaga
     1921 aatataggat ttatatttca aaactacagt ttaatgcccg tattaaacac ttatgataat
     1981 atagctcttc ctgttacatt agataaagga aaccatatag accatgaata tattgaaatg
     2041 ctgatgaaga cattgggaat ttgggataag cgtttaaaat ttccaagcga actatctggt
     2101 ggtcaacaac agagggtagc aattgctaga gctttggcaa ataaacctgc acttatatta
     2161 gcagatgaac caacaggaaa tctcgatagt aaaactacaa tggaagtggt atgtttatta
     2221 aaagaaagta gtgcaaaatt ccatcaaacg atacttatgg taacacacaa tgaaaatata
     2281 gcacaagtat gcgactcaat tatccatatt gaagatggag ttgtggtgaa tactggtggt
     2341 gaagtactat gatgtctcta gtaaaatttt caattcaata tataaagcat tataaaaaac
     2401 aatctatatc tatagtttta agcatagtac tatcagtagc attattgacg ggaattggtt
     2461 ctttagttca tagtgcagac aaaagtagaa ttgaaaagat aagggaagat agtggagatt
     2521 atcattattt ctataaagta gataaggaac aacttaataa aattaaaaaa aataaaaaat
     2581 caaaggaata cattgtagat agacttggcg ttacaaaacc taaaaatcag atagatgaac
     2641 cttttattat agaattttta aatgctgatt cttcatattt agacatgaca ggtagaaaat
     2701 tattaaaagg aactcttcct agtaaagatg gagagatagt acttgacaca tattctctaa
     2761 acaatttaaa tgtaaacaaa gaaattggaa cagaaatcaa tttagatggt aaaatctata
     2821 agttatgtgg tattttatca agtctttcaa acccagaaac tgctatggag ggatttgtaa
     2881 acgattattt gcagcctaaa gaaaaggatt cttatatagt ttatgtaaaa tatgatgaaa
     2941 ataaaaatat taaaaagcaa aattataaat ttatgaaaaa attcaacata tcaaaagaaa
     3001 aaacttatat aaactggcct ctttcagttg aatttggagt aaaaccacca aagaatatag
     3061 aaaaatataa tatatttgag ttcttaaaaa atttagaact aaatacaaat gcaattatat
     3121 tgcttgttgg agtgtttggt gcagcagcag tatatagtat tttccatgtt tcaatacttc
     3181 agagaatatc acaatatggg gttttagaag tattaggcgc taataataaa caacttttat
     3241 ttctactatt tttagagtta tttttacttt ttattgttgg atttcctata ggttgcttct
     3301 taggtattgg agtagcgtct acaatatatg aacaatttcc acatatattt ttgagtagtg
     3361 atattgttcc aggagcattt tttataagtg aaaaatctat ttatttaggc tttttattta
     3421 tcttattgtt gttaatttta attgctgtac gtgttgtaca tcagttgaat aaatactcta
     3481 gtatagaatc tatgaaaaac tttaagatta ctaataagaa aaagagaata ttagaaagtg
     3541 taaaacactc taacatgaca aaagtattat ctcataaata catgactcaa aaaaggggaa
     3601 tgtttattgg aattttgttt tcattatcac ttggtggaat tatatttcta tgttctagtt
     3661 actctataca gcttactaaa aacaataatg aacttacaat gaaagctgat gatggtttaa
     3721 attctgacta ccaaataact atgcaaacaa cagattttga tgtaggtata ccaaaagata
     3781 aaatttcaag attaaacaat gttgagggaa tatcaaaagt ctatccagta agatatttat
     3841 ttggagctat gcaaattaaa gaagaacaac tattttggaa aaatttcttt aaacctcttg
     3901 aaaaagatta caggataaag actttttttg atggtatttg tacaaagcaa aatgaaggtg
     3961 gctatctttt aaaaacaaat atttatggtt ataacaatgg tatgttagaa ggattaaatt
     4021 cttatattat agatggggag attaataccc atgatatggt taaaaataat aaggttatag
     4081 ttagactacc tatggatgga actgggatgt atgatgcagt tgatataaat ccaggagata
     4141 caataaaagt taaagtgcca aagacaatga aaccaacagg tgaaattgtt aaatttaaag
     4201 aagaaaataa gaatgattat acaacaaaag aatttgtagt tgctgctact gtaaaacgtg
     4261 tgatggcaaa taacatatat ttcattggtg attatggtat ggatatagtt atgacgaatg
     4321 ggcaaatgaa aaataacttt gatatcgaaa attataattc agtaagtata aaaaagacag
     4381 aagatagtaa cagtgaggaa ttgtctgaga acataaaaag tgttgtaagt gatgtaaaaa
     4441 gatgtatcgt aacagattat accattgcta ttgaaaaaaa taatacctac ttaaatcaaa
     4501 aactcttatt tatatatggt attgtatttg tacttcttgc aataagtttg ttccatataa
     4561 tcaatacagt aagctactta attttttcaa gaagacatga gtttggaatt ttaagagcta
     4621 tgggtattac ggataataag tttttactta tgatgataag agaaggtttt ttatatggat
     4681 tctatgcaag tattataatg gtaattggaa gtgttattgg acaatttatg atttatttta
     4741 tggtaaaacg tgtatattta tatataaatc caattcttaa aataaataca cctctataca
     4801 taggtatgat tattttaaat atcactatta gtatagttgc tgtaattatt ccagtaagac
     4861 aaattttgaa gagtgatatt atttcagaaa taaataagag ttagagttat ataaattttt
     4921 aagattatat aactttatga
//
LOCUS       DAEPOZ010000248         4714 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.248, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000248 DAEPOZ010000000
VERSION     DAEPOZ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.248"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            368..1228
                     /locus_tag="KR440_003241"
     CDS             368..1228
                     /locus_tag="KR440_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0700247.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKALNIKSSDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSD
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            1218..3089
                     /locus_tag="KR440_003242"
     CDS             1218..3089
                     /locus_tag="KR440_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBH0700248.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRLTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQATTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEASNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            3109..4563
                     /locus_tag="KR440_003243"
     CDS             3109..4563
                     /locus_tag="KR440_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBH0700249.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYETPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLMDLYLKYSETVI
                     RRFDDKVKYWITFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
BASE COUNT         1807 a          500 c          880 g         1527 t
ORIGIN      
        1 ttcattatat ttaaaatcat tactatgata attcataata gtaaaataga taatggttca
       61 gacaatattg taaatattag tgacagtaaa atagtatcaa tttagtatat aaactttgcc
      121 agataatcgc ataacagagc ttgtagttat agaataaaaa aatatctagg aaaccgttga
      181 cagatttata tggatagtat aacatataga atatgaaatt aaacaaaaaa gaattttaat
      241 tatggcgacg tagtcataat tttgagtatg ttactgatta gcaggcagaa ctcaagtata
      301 tatgtgaaaa atagagggat actctataca tatatgcttg agttcttttt attgaaagga
      361 ggaatatatg aaaataattc agattttaaa taataatgta gccttagtaa aaagaggtaa
      421 aaatgaaatt attgtatttg ttaaaggaat tggctttaaa aaaagagtgg gacaagtaat
      481 aaatgaaaat gaaatagaga gaacatatat actagattca tacgatatgc tagaacactt
      541 tagttatcta ctacaaaact cagactccaa taatatagtt cttattaatg aaataattga
      601 atatggtgag aaagctttaa atataaagtc aagtgattat ctaagcttaa ccttactaga
      661 ccatttagag tttttactta aaagatgtga aaagaatcaa tttataaaaa gtcctttggt
      721 atggaatgtt aaaagatttt atccaaagca ttttgaaatt ggaatgcatg cactaaaatt
      781 aattggtgat agtaagggaa ttaatcttcc tgatgatgag gcagtatcaa tagcacttca
      841 ttttgtaaat atggaggtaa ataaagagtc tcatgatagt acaattgtag agttgagaac
      901 tttagcagat atagtttcta taataaaata tcattttaat gtagagctag atgaaacttc
      961 aacaaattat atgagattta ctactcatct acagtacttt atccaaagaa ttatgtcaga
     1021 tgaagcttca atagatgaag ataattctat agatttatat aaacaagtaa gtcaacttta
     1081 tccaaagtca tttggaagtg ttcaaaagat aagaaaatat ataaaatcac aatttaatgt
     1141 cgatatatct ataagtgaag aaacttattt aatgttgcat ataaataggg tgactcaaag
     1201 actggaggca aaatcagatg aaatataaag atttaaatga aaatataata aaacttatag
     1261 ggggaaaaga aaatataaag tcggtagcgc attgtgttac tcgtttaaga cttactttaa
     1321 atgatagaag tatcgctcaa acagaagaaa ttaaaaaatt aaaaggagta attgatgtag
     1381 tttctaatga tgttgcttat caaattataa taggtactca tgttgtagat gtatataacg
     1441 agtttatgtc tatgataggt ttaagttcaa gtgaatcaag tataaaaagt gaaagaaaaa
     1501 agggaattag aggtatcaaa ggtatattta attccatctt tgtggtagtc tcagagacta
     1561 tgacttcagt aattgaagta ctacttgcag ctggtatact cgcaagtata ttagcacttt
     1621 taaatatgtc tggcatactt cagagtgata gccctacata tcaaatatta gatacattaa
     1681 gaagtgctac atttcacttc ttaccagtat taattgcaat agcatcagca aaaagactta
     1741 atgtaaatcc ctatatagca gtagtgttag cagttacgtt attatcgtca tcaatagatg
     1801 gtgtagaagg attaagttta tttggtataa atttacaggc tacaacttat gcaaacagtt
     1861 ttattccaat acttttagga gtatgggcaa tgggaattat aattgaaggt ttgaaaaaaa
     1921 tattacctaa agcactacac tattttttag ttccagtgtt atctttaata ataaccctat
     1981 cagttacatt aactgtattt ggacctattg gtatgtggat tggtgatgga ttaaactatg
     2041 catgtatgtt tttaattgat acattgggta attggagtgt tgttgcactg tatgcagctt
     2101 tacaaccact cttaatagtt tttggtgcag caaactttac tatgcctata tctttaaatt
     2161 ctgtgacaac attggggtat gacccaattt ttatgggcgc tgcaacaata tctgatattg
     2221 cagtttgtgg tgcaatgttt ggatatttct taaaaactag gaataaagaa caaaaacaat
     2281 tatttgggac agtaagtttt agtgcactta tgggagttac agagccttca gtgtttggtg
     2341 tgtttatgaa atatagaaga ccatttgtag cagtaatgat aggtggaggt ataggtggtt
     2401 taatagcagg tttagctcaa gttaaaacat atggaatggt ttggggatta gctgcattac
     2461 caacatatct tgttggagga gaggcatcta actttacatt tatgataata agtgtagtag
     2521 tatcatttgt agcagcaaca gcagcttcct atttactagg aataccttca gatgaaaaag
     2581 aagaagttaa gggtgataat gaaaaagaat tagaactaat aatcaacgag aacaattcaa
     2641 gaactattag tattggaaat gttgcaaaag gtcaggtaat tgcacttggg gaagttaatg
     2701 acaaagcatt ttcctctgga gcattaggta aaggggttgg agttatacct agtgaatcaa
     2761 aatcaactgt tatatctcct gtagatggaa ctataactat agtatttcca actaaacatg
     2821 catatggtat agtgactgat gaaggattag aaatattaat tcatattggt atagatactg
     2881 taaacttaga tggaaaattc tttgaaagtc ttgtaacaca aaatcaaagg gttaaaaaag
     2941 gagatgcttt agcagtattt gaatctgata aaattataga ggaaggcttt gattctacta
     3001 taataactgt agttacaaat actagcgatt atttagatgt aatctcatca aatgatgaat
     3061 cagaagaatt attaactgta atagtataaa ttttaggagg gaaaattcat gaatattaaa
     3121 gatacatttt tctggggagg aagtattgca gcccatcaat gtgaaggttc atgggatagt
     3181 gataacaaag gacctgcaat tatggatttt gtaactaagg gctcttatga aacgccaaga
     3241 gtaattactg ataaaataga agaaaaattg gattatccat ctcataatgg aatagatttt
     3301 tataatagat ataaagaaga tatagcttta tttaaagaaa tgggatttag tgcattaaga
     3361 atatctatag attggtcaag aatttttcca aatggagatg atgaaaatcc taatgaatta
     3421 gggattaaat actatgaagg tctgatagat acattaatag aaaataatat agaacctatt
     3481 gtaacactat atcattttga attacctatg aatttagtac ataaatatgg ttcatggaat
     3541 aacagaaaac ttatggattt gtatttaaag tacagtgaga ctgtaataag aaggtttgat
     3601 gataaagtaa agtactggat tacatttaat gagatgaacc atattgaccc tcaaacagag
     3661 cactcggata tttttactta tatgatagca ggtattaagt atagtgaaat agaaaataag
     3721 gttcaaacat tggcaaatgt agggtataat atgactttgg cgggtgtaaa ggttgcaaag
     3781 ttggctagag aaatcaatcc caacaataaa gttggatgtg tatttggact aaatccaatt
     3841 tatccataca attgtaatcc tagtaatgtg ttaaatgcat ttttagataa tgatcgtgat
     3901 tattatcaaa ttgatgcaat gtgcaatggg aagtttcccc aatataaatt aaaagaatat
     3961 gaaaaaaata atataactat tgaagtgact gatgaagaca aaaaagcatt taaagaaggt
     4021 aagttagatt ttataggtct taattattat ttttcaagtg tatctactcc aaaagaaaat
     4081 ttatcaggtg ataaatcttt atttggtggg attcaaaatc catatttgga acaaagtaag
     4141 tggggatggg caatagaccc tataggttta agatttacct tgaactatat ttacagaaag
     4201 tatcaattac ctatcttaat tacagaaaat ggtttagggg catatgataa aatcgaagaa
     4261 gatggaacta taaatgatga ttatagaatt gaatatttaa gtaagcatct atctcaaatg
     4321 aagtctgcaa ttgaagagga taatgttgaa tgttttggat atttgatgtg gggaccaatt
     4381 gatttagtca gtgcaactac aggtcagatg agtaagagat atggatttat atatgttgac
     4441 ttagatgatg aaggcaaagg aagttttaag agatataaga aaaaatcatt tgattggtac
     4501 aaagaagtaa taaaatctaa tggagaagtt ttagattttt ctacggatat agtagtaggg
     4561 tagtaataag ataaatagaa gttaggaact aatgttaatt agtgtctagt aaagcattta
     4621 aaatagagta tagggtcttt aaagtaaagt gtgtattctt attaaaagta atggcaacta
     4681 aaattactat aaaaaggaga atacacattt ttta
//
LOCUS       DAEPOZ010000249         4710 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.249, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000249 DAEPOZ010000000
VERSION     DAEPOZ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.249"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..663
                     /locus_tag="KR440_003244"
     CDS             <1..663
                     /locus_tag="KR440_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBH0700250.1"
                     /translation="DKVIIFNNLSTKTKNQLKEKAKLKRLQKKEILFYEKDSLDSVYI
                     LLEGKVSIFKISENGERKVIFILNSGEVINDITFDSSKNSTVGCEAFENSIVAYYSIE
                     EFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYRLSREFGVCC
                     GEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVKETELSRYFK
                     NN"
     gene            1053..2060
                     /gene="asrA"
                     /locus_tag="KR440_003245"
     CDS             1053..2060
                     /gene="asrA"
                     /locus_tag="KR440_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HBH0700251.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEIIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
     gene            2061..2852
                     /gene="asrB"
                     /locus_tag="KR440_003246"
     CDS             2061..2852
                     /gene="asrB"
                     /locus_tag="KR440_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HBH0700252.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            2865..3836
                     /gene="asrC"
                     /locus_tag="KR440_003247"
     CDS             2865..3836
                     /gene="asrC"
                     /locus_tag="KR440_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HBH0700253.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            3893..4669
                     /locus_tag="KR440_003248"
     CDS             3893..4669
                     /locus_tag="KR440_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBH0700254.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGLIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     STVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
BASE COUNT         1878 a          421 c          890 g         1521 t
ORIGIN      
        1 gataaagtaa taatttttaa taatttaagt actaaaacga agaatcaact taaagaaaaa
       61 gctaaactaa aaagattaca aaaaaaagaa attttatttt atgaaaaaga tagcttagac
      121 agtgtttata tacttttaga aggaaaggta tctattttta agataagtga aaatggagag
      181 agaaaagtaa ttttcatatt aaatagtggg gaagtcataa atgatattac atttgactct
      241 tctaaaaact ccactgttgg ctgtgaagca tttgaaaatt caattgtagc ttattatagt
      301 attgaagaat ttttagaaat tatgcaaaat gattttcaac ttacaaagga tattatttct
      361 tatatggaaa gaagaataag acgtttatat agacagttga aaaattctat atccataaaa
      421 gtagataaaa aacttgcagc aaaattatat aggttaagta gagaatttgg agtttgctgt
      481 ggagaatgga ctttactaaa tgtaaatatt acagttacat atttagctga tatgcttggc
      541 tgtaaaaggg agactgtttc cagaatgata aaggtattgc aaaaagaaga acttattaag
      601 attgataata aaggttttta tgttaaagaa actgaattat caaggtattt caaaaataat
      661 taattatgat taagtaaaag tattattaga attttaatta aataaaaata ttattaagag
      721 aatctattta tagattcttt tttattgtct aatttattaa tttttatttt accctattga
      781 attgtataaa aaagcaaata agattcttta ttattcaaaa aattgagtag ataatagttt
      841 tattgtattt aatgattgta tctaagtgat tagatttgaa atcgaataat atagataata
      901 aaaaaatatt aacagagatt agtaaactct attaatattt ttttattatt tttgaaactg
      961 tgataattat cacagaagca tagaaaataa agtgttaaga tttaaacaag atagcaagta
     1021 agtaaaacta aaatataaga aaggtgataa aaatgaaact aaaattagat tcatctaagt
     1081 tcaatgaagg tctgaattat ctaaaaaaag actacaagat atttgcacca gttttaatcc
     1141 catttaaagg tactttttca gatactgatg ttataagata caaggaggta aacgcttttg
     1201 aagaaatgga atttgctaag aaagcaaact tttcaccaaa agaagttgta cttcccataa
     1261 atcaagtatt gttttatttt actgaaaaag agtttaagga aagcgattta gacaacaaga
     1321 aaattttaat tttcttaaga gcttgtgaca taaatggtat aaaaagactt gatgaaatat
     1381 atctaaataa tggtgaagaa aaagattatt tttataaaaa tatcagagat aaagtaaagt
     1441 ttgcacttgt agggtgtaag gaaagtttta gaaactgttt ttgtgtgagc atggattcta
     1501 acaaaactga taattattca attggattaa atatagaagg agatacactg tacttagata
     1561 taaaggatag tgaatttgaa gtatttaatg gagaagcaac tgaatttaat gttgattatg
     1621 taactgataa tttaatatct gtagatgtgc cagagaatat tgattcaaat gaaattatag
     1681 gaaattcaat atgggatgaa tatgatacaa gatgtatagg atgtggtaga tgtaattttg
     1741 tatgtccaac atgttcatgt tttacaatgc aggatatttt ctataaggaa aacgaaaatg
     1801 taggtgagag aagaagggtt tgggcttctt gtcaagtaga tggatataca gatatagcag
     1861 gtggaaattc ttttagaaaa aaacaaggtg aaagaatgag atttaaaact atgcataaaa
     1921 tacatgattt taaaaaaaga ttcggatatc atatgtgtgt tggttgtgga agatgtgatg
     1981 atgcctgtcc gcaatacata tcattttcaa agtgtataga aaaaataaat gatttagtaa
     2041 ttagtaagga ggaagtataa atgaacccat atataccagt aagtgcaaaa atactagata
     2101 tagttaaaca tactgataaa gaatggactt ttagagtaaa ctgcaataca aaaggtgtat
     2161 tgcctggaaa gttttatgag atatctatac ctaaatacgg tgaaagtcca atatcagtat
     2221 caggatatgg agaggattat attgacttta caataagaaa tgtaggaaaa gtaactagcg
     2281 agttgtttaa ttataaagaa ggagattctt tttttataag aggtccttat ggaaatggat
     2341 ttgatgtatc cctttatgaa ggaagagaaa ttgtagtagt ggcaggagga agtgcattag
     2401 cccctgtaag aggaatagtt gaatattttt ataataataa agaaaaatgc gaaaagttta
     2461 aattaatagt aggatttaaa tcaccaaaag atattttatt tgcagatgat ttaaagagat
     2521 ggagtaaaaa acttgatatt ttagttactg tagatggagc agaggaagga tatagtggta
     2581 atgtaggatt agtaacaaaa tacataccag aattggatat gaaagatata aacaatactt
     2641 ctatagttgt agttggacct ccaatgatga tgaaatttac agtagaagaa tttttaaaaa
     2701 gagatttaga tgaaaagaac atttgggttt cttatgaaag aaaaatgtgt tgtggaatag
     2761 gtaaatgtgg acattgtaaa atggatgata cttacatatg tttagatgga ccagtatttg
     2821 attattcata tgctaaaaat cttattgatt agggggcgaa gtttgtgata agagatttaa
     2881 ataccaaaaa agtaatgaaa aatgcttata gaattacaaa aacaaaatat gagacttctt
     2941 taagagttag agtacctggt ggtcttattg accctgagag tttagctgta gtttctaaaa
     3001 ttgcaagtga gtacggaaat ggacaggtgc acataactac aagacagggg tttgaaatat
     3061 taggaataaa tatggaagat atggaagaaa taaataaaat tatacaacct gttattgaaa
     3121 aaatgaatat aaatcaaagt gagaaaaacg caggttatcc agcagcaggt acaagaaata
     3181 tagcagcttg tataggaaat aaagtctgtc caaaggctca atataataca actgaatttg
     3241 ctaaaagaat agaaaaagct atatttccaa atgatttaca ttttaaagtg gctttaactg
     3301 gttgtccaaa tgattgtata aaagctagaa tgaatgactt tggaattatt ggtatggctt
     3361 taccaatata tgaaaaagat agatgtgtaa attgtggagc atgtgtaaaa aaatgttcta
     3421 aaatatctgt tggagcatta aaaacagaaa ataataaggt ggtaagagat aaagataagt
     3481 gtataggatg tggagagtgt gtactaaatt gcccaacaaa tgcatggact agagatgaga
     3541 aaaaatacta tagattagct ataatgggta gaacaggaaa gaaaaaccct agactagcag
     3601 aagattttct tttatgggta gatgaggaca gcataataaa gataatactt aatacttata
     3661 gatatgtaga aaagtatata gataaagatg caccaggagg aaaagaacat ataggatata
     3721 taattgatag aacaggattt atggaattta aaaaatgggc acttgaaggt gtagagcttc
     3781 cagaaataac taaaatgtac gaaaacatat actggagtgg aattaaatat ctataaattg
     3841 agtaaaacat atatttgaat aaattaaaat ttaatagaag gaagagggaa ttatgcataa
     3901 agaaactttg gataaattaa ctaatgcagc tataaataag ataaatttat taaacacaag
     3961 taaagtaaaa tatttggtat cttctgcgtt tgcaggactt tatgtaggta taggtatact
     4021 tttgatattt actataggag gacttttgac tgatgcaggt tcacctatga ctaagatagt
     4081 aatggggtta tcatttgcaa tagcactaag tttggttata atgacgggaa cagagttatt
     4141 tactggaaac aatatggtta tgtctgctgg tatgttaaat aaaggtgtaa gtataaaaga
     4201 tacttctaaa atatgggcat acagttgggt aggtaattta atcggagctt tggttttagg
     4261 tcttatattt gtaggaactg gtctagtaga caaaggacct gtagctgaat tttttgcaaa
     4321 tacagcagct agtaaagcat ccatgccatt tacagctctt ttctttagag ggattttatg
     4381 taatattcta gtatgtgtat cagtgttgtg ttcgtttaga actaatagtg atactgcaaa
     4441 aataattatg atattcttat gtttatttgc ttttataaca tcaggttttg agcatagtgt
     4501 agcaaatatg acaatttata gtgtttcatt attttcacca acaataagta cagttactat
     4561 tggaggagct atttataatt tagtagctgt aacacttgga aatattgttg gaggagcttt
     4621 atttatgggt cttggaactt atatattagg taaagaaaag cttaattaaa tttattgtat
     4681 aaattataac tctcttgata aatacatttt
//
LOCUS       DAEPOZ010000250         4710 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.250, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000250 DAEPOZ010000000
VERSION     DAEPOZ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.250"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            176..682
                     /locus_tag="KR440_003249"
     CDS             176..682
                     /locus_tag="KR440_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HBH0700255.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTVAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
     gene            1095..1763
                     /locus_tag="KR440_003250"
     CDS             1095..1763
                     /locus_tag="KR440_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700256.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKEEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSSKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWENDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
     gene            1922..2428
                     /locus_tag="KR440_003251"
     CDS             1922..2428
                     /locus_tag="KR440_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HBH0700257.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            2556..3572
                     /locus_tag="KR440_003252"
     CDS             2556..3572
                     /locus_tag="KR440_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBH0700258.1"
                     /translation="MGYYNDNDNNKIRRKKISNNKDSNKKSKHSYTSANKKNKSPNTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            3926..4630
                     /locus_tag="KR440_003253"
     CDS             3926..4630
                     /locus_tag="KR440_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HBH0700259.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVIICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
BASE COUNT         1988 a          411 c          797 g         1514 t
ORIGIN      
        1 tatattttac acttataaaa tatgaaaact acttattgcg aaagtaattg atttgttgta
       61 ttataattta catgaattat gtaggacaat caaaagtcag tattataaga gtacatatat
      121 tcaaattttt aacaattaat ttatataatt aaaaaatgaa gggtaggatg aagtcatggg
      181 ttttagagaa gtaaaaattg aggaactaca gtttaatcca tttactaaga ttggcaaaga
      241 atggttactt ataacagctg gtgattcaga aaagtttaac acaatgactg ctagctgggg
      301 tggtgttgga gtctattggg gtaaaaatgt tgttacaact tacataagac cacagagata
      361 tacaaaagaa tttgtagata gtaatgatac atttacagtt gccttctttg atgaaacgta
      421 tagagaggct ttaaatattt gtggtactat ttctggtaga gatataaata aaatagaaaa
      481 agcaggactt acaccatatt ttgtggatga tacggtagct tttgaagaag ctaatatgat
      541 tatagtatgt aaaaagctat atcatggtaa tatgccacca gaaaactttg atgctaaaga
      601 aaatgataaa aaatggtatc cagagaaaga ctatcatact atgtacattt cagaaattat
      661 aaaagttctt gtaaaagaat aattataata aaaaatatat gaaaagaact tattataagt
      721 aaatgactgt gctttcttat ctgatatcta agtagatgct actaactaaa taaatattaa
      781 ttttaaataa atactattat aaaaaagatg aactatattt actatagttc atctttttat
      841 ttatataagc acttatataa gtagagattt atttgaataa aagtatttat ttgaaatatt
      901 tgagcatact ttataagtat ttttatgcta actagaaaca cttaatttat ggcatttgta
      961 tttgatttat tttaatgtaa ataatttcaa agttaatttt attcaatata gtactataaa
     1021 agtataaata acatttaata aaaatatata ttatattagg tattttaaat aaatttttta
     1081 aagggtgatt ttttatggaa aatataacta gttattttac aacaatacta tgtgtattta
     1141 tatgcttatc atctgtattt atatttactc aactagctag agtatttatt aataagaaga
     1201 agattaatca aaaaattaaa tcaagaaacg gatttaggta tgatagagat tttattgaag
     1261 ccagaagaga agaaatacat ataaaggata acaataataa taaaaataag agtaacaata
     1321 agaaattaaa ggaagaaaaa gtatttaaat acgataatgg tgatttatat aaaggtgagt
     1381 ttgttgatgg aaagaaaaat ggatttggaa tatatatttt ttcaagtaag gaaaagtatg
     1441 aaggattatg gaaagatgac aagatgcatg gaataggaaa atatacttat agagatggtt
     1501 caatatatac aggtgaattt aaatacggat taaaaaatgg tttgggaaaa ttaacatatc
     1561 ccaataatga catttataag ggatattttt tagataataa gaaaaatggt aagggtgtat
     1621 tatataaaaa tgatggaaat aaacaagcag gaatatggga aaatgatgaa caatgtaaaa
     1681 gtttagattt taaagattta aataataata aaataaataa tgtttatcaa aatagattaa
     1741 ataacaataa attaaaagtt taattattct aataaaaaaa tctttattta tgatggaata
     1801 taggatatat ctatgttgta atttgtaaaa tcttcctata tcatataaaa ttaagtattg
     1861 attagtctat tacataaata tagaatataa atatatccat ttaataataa ggagaagaga
     1921 tatgaaagaa tatgtagttg tggatttaga aactacagga ttagatccat ataaaggttg
     1981 tgaaataata gaaataggaa taacagaaat aaaaaatgaa caaattgtaa aaaactattc
     2041 aaggttgata aaacctaagg ggataatttc ttcatttata acagaattga cacatataag
     2101 taatgaaatg gtagaaaatg aagaaccact agaattagtt ttacctagat ttagaaaata
     2161 cataggtgat agaactataa ttgcacacaa tgcgaaattt gatttaaaat ttttaaatta
     2221 ctatcttagg atgttagatt tagaaccaat aaacaattat atatgtacag tagaattatt
     2281 aaaaaaatgt aaaagttata aaggaaaaaa caagaaactt gaaacagctt gtgcttatta
     2341 caacatagag aatataaatg ctcatagagc tgacagtgat acattagcaa ctgcaaaact
     2401 atttttgaaa ataaaagatg aatattaatt ataaatatag agaaaattag tgaaaatatc
     2461 ctttataaaa ttgaccctta agcttacaat tttgttcttt gatttatttt gtagaacaat
     2521 gtataatgta ccttatatga agaagggaga ctaaaatggg ttattacaat gataatgata
     2581 ataataaaat aagacgtaaa aaaattagta acaacaaaga tagtaataaa aaatctaaac
     2641 attcttatac ttcggcaaat aaaaaaaata aaagtcctaa tacaagttcc agtaaaagaa
     2701 tgagtgaaaa agtagattca cagaatctta taaaaagaga tagagatagt tttacaagag
     2761 ataatgtaag acctttaaat acagattaca atcaaagaga aagaagaaga gtcgagtata
     2821 aacaaagaga gaaaaaatta aaaagtaaaa aaagaaaaaa acaaaaaagg attagaatag
     2881 ttagggcatt attatctatt atgttagttg cactaattat attttcagtg gcaggtgcaa
     2941 tatttacagt ttcagctata aaagggtcac cagaagttac taaaaagtta attaggtcta
     3001 aatatataag tagtgaggtt gtaagtataa aacaaatgcc aaatgattta aaacatgcaa
     3061 tagtttccat agaagatgag agattttata agcataaagg agtagatgtt atttctctag
     3121 ttaggtcagt attaaacaat gtgtttacag atacaacaca aggtgcaagt actatagata
     3181 tgcaagtttc aaaaaacctg cttactagta atgataaaac tatgaagcgg aaaataaggg
     3241 atatgtataa tgctaagcag atgaataaga ttatgactaa ggatgaaatt cttgaggcat
     3301 atttaaataa tatgtatcta ggtaaaaatg catatggagc tgcaaaggga gctgaagtct
     3361 actttggtaa aaaagttagt gaattaaatt tagctgagtg tgctatgtta gctggtatga
     3421 caaataatcc tgcaaggtat attgaccatg gagaggctaa gaagagacaa gtgattgtac
     3481 tgtataagat gcatcagctt ggttatataa ctgataagga gtatagaata gcaaaagctg
     3541 atgacactcc atttaaatca gaaattgatt aaaattgtaa gaatatagta ttttcaatag
     3601 tttaaacaaa caaaaatgta ataaaaaagg tgatttgcca cccatttgcc accataatta
     3661 taatctaatg gcaaattgtt ttaatgtaca aactagataa tagaatattg ttttaaatgc
     3721 tatattatgc taaaatttaa ttgtattttg taaattaaaa aacttataat ttagttttct
     3781 agggttccgc attttataat ggtcaggtcc aagagaaaac gcgcaattgt tgtgtacacg
     3841 gaaggataaa agcctgggag atatttaata atatttctat aggctttttt atttttacat
     3901 attttaaatt tgaggaggtt ttctaatgaa aaaatttgtt attgaggatg cattttggaa
     3961 tctatttcct agtgcaaaaa ttggagttat tatttgtaat gatatagata actctataaa
     4021 agatgaagac tataagaaaa taattttgaa gggagaagaa gaagcattaa aatacttgga
     4081 ggactcagaa tttagcaaca accaagtgat taaagtatgg agagaggcat ttaaaaaatt
     4141 taaaacgaaa aaaggagcaa ggtcttctat tgaagcatta ttaaaaagag ttcataatgg
     4201 aaataatata ggaactatta accctttagt ggatatctac aattttatct cattaaaata
     4261 tgcattacct tgtggagggg aggatataga taaatttata ggagatatta ggctaactag
     4321 agcaattggt aatgaggagt ttatcccact agggacagat gaaaattcat ttccatatga
     4381 aggagaaata atttataaag atgatggtgg agcaatttgt agatgttgga attggcgtga
     4441 agcagtgaga acaatgctta ctgaagatac taacaatgcc tttttatgca ttgaattagt
     4501 agatgaaagt agatttaaag aatttgaaaa tgctttaaat gaattagcta aaatagttca
     4561 agaaaaattg ggtgggaatt gtaaaatttc aattttagat attgataata aagaggtatc
     4621 agtggattaa tctggagatg ctgttgtaat ggactattct agttataaag atacttatat
     4681 ttagggtaat aagttctatt ttagatgtaa
//
LOCUS       DAEPOZ010000251         4696 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.251, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000251 DAEPOZ010000000
VERSION     DAEPOZ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.251"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            202..1008
                     /locus_tag="KR440_003254"
     CDS             202..1008
                     /locus_tag="KR440_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBH0700260.1"
                     /translation="MGMILSTLGINLYLAPAKLLSGGVAGICVILYKLFGINQGISSF
                     LMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLTQDIAKYFPIDDTMLQCIYGGAL
                     TGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNTFLFINFFVVCAGAFLFGAKLVM
                     YTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEISKVIMNKMGRGVTFLEAEGAYT
                     QNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNNVEEVKGGGFKDKFL"
     gene            1135..1407
                     /locus_tag="KR440_003255"
     CDS             1135..1407
                     /locus_tag="KR440_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700261.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(1570..2142)
                     /locus_tag="KR440_003256"
     CDS             complement(1570..2142)
                     /locus_tag="KR440_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0700262.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            2326..3168
                     /locus_tag="KR440_003257"
     CDS             2326..3168
                     /locus_tag="KR440_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBH0700263.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEIGSVMGTYTSKGAIL
                     ITAI"
     gene            complement(3271..4347)
                     /locus_tag="KR440_003258"
     CDS             complement(3271..4347)
                     /locus_tag="KR440_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBH0700264.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
BASE COUNT         1727 a          593 c          720 g         1656 t
ORIGIN      
        1 ataaatatat taaaagaagg attaagacta ataacaattc aaaataaata acttatgtaa
       61 gaaccgtagg aactacgggg atagcttgtg gagaattagt aagacatatt ttagtatgca
      121 aaattctatg aagcaggaac cctgtgactt tagtcatggg aggttcagaa taacatattt
      181 ttacaatatg gtctgattat attgggtatg attctatcaa ccttaggaat aaatttatat
      241 ctagcaccag ccaagttatt aagtggagga gtagctggaa tttgtgttat attatacaaa
      301 ttatttggta taaatcaggg aatatcaagt ttcttgatga acataccaat atttattata
      361 gctagaaagt attttgataa taaatttttg ctaataagtt ttgtcaatat gttactgttt
      421 tctctagcac tggggctaac acaagatatt gctaaatatt tcccaataga tgatactatg
      481 ctacagtgta tttatggtgg tgcattaact ggtataggta tggggttaac ttttaaagca
      541 agagctactg ctggaggact tgatataata gcagccataa tgaaaagaaa gtatgatata
      601 cctatgaaaa atacattttt atttattaat ttttttgtag tatgtgcagg agctttctta
      661 tttggagcta aattggtgat gtacacttta attacaatgt atataatttc ttttactatg
      721 gatataggaa aagattgctt tgatagaaaa aaatctatac ttttaatatc aaataaatat
      781 gatgaaatat ccaaagtgat aatgaataaa atgggtagag gagtcacttt tttggaggca
      841 gaaggtgcat atacacaaaa taagaaaaag atgatttatt gcataatatc tgcaaatgaa
      901 ataggtaaat ttaaagattt aatttatgat atggatagtg aagcttttat ttctgtcaat
      961 aacgttgaag aagtaaaagg tggaggtttt aaggataaat ttctttaaga ttatatatgt
     1021 taattatata tgttaaaaaa tgccatatgt attgagatta tacctagtta gatgtatact
     1081 gtagttaatg gggtacatga agtattcaat aatatactag gaggtataag aattatgtta
     1141 agagagagat ttgtagtaag aaatcatcat aatccacata caagtgggtg ttgtgggact
     1201 ggaggagaag gatgctgtgg aggagataac catgaacatt cacatcaaca ttctcacagt
     1261 catgggcatg aacatgagca tgtaaatggt gtaggtcacg accataatca tgatgatggt
     1321 ttttcaggta ctcatgagca tagccatgag catacacata cacatggtca tgaacattca
     1381 gatgggcata cacatgaaca taaataaaaa cattttaaat ttataagaga tttttatttt
     1441 gtgattccat gaaggtttaa ataaaaattg aatgttttaa gtaaaaatta ttgagaataa
     1501 ataagaaagt gaactgttaa agtagataac ttaaatagtt cacttttatt aattatctaa
     1561 gtattaagtt taaatccaat gattaactaa aaaagaagct atattttcat aaggtatttt
     1621 ttctgttttt aataaacttt ggacaactaa accatcaact attgacatga gtaaaaaaga
     1681 aaatgattca gcatcctgtt cattttcgct actattatat ttttctagtg attcaacaat
     1741 ttcagattta agagtagttc tccattcaat atattttaat cttattcttt cctttaaatc
     1801 attatttgaa gtaattgcct cacaaataag atacatatgt accctaccac gactttctat
     1861 agtagatatt ttctctaaga ttaagttaac catttctatt tgagaatttt ttgatttgac
     1921 attttttaca cagtttagta tagcactagt tataacttct aaatgagtat ccgcaatatc
     1981 atatataata tcatctttag atgcgtagtg ataatacaaa gtacctttac ttatatttac
     2041 atcttttgca atatcagcaa ggctagtatt ttgaactcca tttttggcta ttaaattggt
     2101 tgcagatgat aaaatagcct gtttcatatt atttgttttc ataaaaccta cctttccatg
     2161 tatatttata acaaattaat aacattataa gcttatgaat atttattgtc aataaagcga
     2221 gaatctatat ctattgactt tttttcattc aatatataaa ataataatta aatcggtcgt
     2281 acgaccgact gaataaataa aataatatct tatagtgagg ttaatatgga taaaataaaa
     2341 ttgatagtgg acagtgcatg tgacttacct gatgatataa tagaaaaata caacatagaa
     2401 gttgttggtt taaatgtatc ttttggagaa gaaagttata tatctggaaa ggaaatagat
     2461 aatgaaactt tctatagaaa aatgagtgag agtaaagtac taccaaaaac atcatgtcct
     2521 tctcctgata aatttttaga agcataccat tgtcaggaag aaagtgtatt ggtgattaat
     2581 ataagttcag gattatctgg gacttataat agtgcacttt tagcaaaaga tatgtttgaa
     2641 aaggaaggaa ataacaaaaa agttgaggtt atagattcac ttagtgggtc aattggaata
     2701 ggacaattag tgttaaaagc agcaatatta ataaaagaag gaaaagaaat tgaagagatt
     2761 gtttcaataa taaataaata taaagataat cctccatttt ttggaacact tgaaacttta
     2821 gaaaatgcta ttaagggtgg tagaataaat ccaatagcag gaaaaattat aaataccttg
     2881 aattttaagg cgataataca agttgcagat ggcgttgtaa ctcctataga taaggcaaga
     2941 ggtgaaggaa acagtttgaa aaagctgatt actttagtag agtcaaaaat taaagataaa
     3001 gaagaaaagg ttttatttat aggacatgct aattgcccag aaaaggctca aaaggtaagg
     3061 gaagttatgg aaaaggatgt aaaatacaaa gatgttgtaa tctgtgaaat tggttcagtg
     3121 atggggacat atacttcaaa gggtgcaatc ttaataactg caatataaat aatggattga
     3181 aataaatcat agattgagaa attaaagcca tataaaaatg tttaaaacat ttttatatgg
     3241 ctttttaatt ttatcaaatt gattttagaa ttaaccaact acacatattt ctttgtcatg
     3301 tttgtttaat atctcacaac catcttcagt aacaagtact aaatcttcta ttctaactcc
     3361 gaactcacct ggtaaatata tacctggctc tatagagaat atcattcctg gttgtacagt
     3421 atctgtattt atagaagata catctccttt atcatgaact tcaagtccta tagagtgacc
     3481 agttctatga gtgaagtact gtccatatcc cttttcagtt atgtagtcac gagcagcagc
     3541 atctatatca cagaatctaa cacctggttt tacaatagct tcagctctct tattagcttc
     3601 aagaactatt tcaaatattt ctctaccttt ttcaggtatt tctttataga atacagttct
     3661 tgtcatatca gcacaatagt tatcttttat acaacctata tctaatatta ttgcatctcc
     3721 tggtttcaca agtgcatttc caggttcacc atgtggatta gctccattag gtccaaaacc
     3781 tataataggg tcaaatgaaa atccatcagt acctaggtct tcatatattt tagataattt
     3841 accaacaagt tgtttctctg taagttcacc agaaacagta gctttaagtt gttccatagc
     3901 cttgtcattt aattttgaag ctattctcat taactctttt tcttcttcat ctttacacat
     3961 tcttaaagta tctattatat aagatgaatt tacaaactta cttcctccac ctaattctat
     4021 taagtttaat aagaatctag ctggccagtt tttatcaaca cccatagtag catttttgtc
     4081 tatatgttca gttattattt ctactggatt ttgagtatca ttaaaccaaa ccatttcaac
     4141 acctaaatct tctgaaactg ggaataattc atttataaat aatttattat tgccatttaa
     4201 atttaagtat aatgctaata atctttcacc tggatggatt agtactcctg taagataaaa
     4261 tatagaagta gggtcactta ctaacatttg agaaatatca tcttttttca tttgttcaag
     4321 tacagcattt aatctttgag ctttcataat aatttcctcc taatcaaaat tcaatacaaa
     4381 tcaattttag cattattaaa caaaaaagag tatacttttc ttctagaaat ataaaaaaag
     4441 tttgttatat attttttata ttaagtttaa tctctacttt tgcattaaat acggaagtaa
     4501 aatgactttt tattgtgaaa attctataaa agtaataaat taagtctata aagttatgta
     4561 ttttatcttt ttatatacta atgaaaaata ataaataaaa ttttataata aaataatatt
     4621 gaaacaattt taaaaattta gtatcattag aattaaagtg gtaaaacttt taaatttatc
     4681 atttttaata agattt
//
LOCUS       DAEPOZ010000252         4667 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.252, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000252 DAEPOZ010000000
VERSION     DAEPOZ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.252"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(204..1388)
                     /locus_tag="KR440_003259"
     CDS             complement(204..1388)
                     /locus_tag="KR440_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBH0700265.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMIKLVNAVPVFIDTKKENGFKLTKEELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1761..4112
                     /locus_tag="KR440_003260"
     CDS             1761..4112
                     /locus_tag="KR440_003260"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HBH0700266.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERFEVQGNKKMKNFPFLMGQGVWKGSDDLVPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4134..>4667
                     /gene="nrdG"
                     /locus_tag="KR440_003261"
     CDS             4134..>4667
                     /gene="nrdG"
                     /locus_tag="KR440_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBH0700267.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIA"
BASE COUNT         1797 a          547 c          759 g         1564 t
ORIGIN      
        1 ttataataaa tttagaattt aacttatata gtttaatttg ataatttgat gaaaaaaata
       61 aaagacttac tgatttttaa atctataggt cttttatttt ttatctaaca tttaaattaa
      121 gttgttgaat atatcttata ttacttactt tatatattaa ttaccattaa tacattatat
      181 ctaattacta ttcatctaaa ttattatgcc attattttat atacaaattc ttttaatttg
      241 tctaatccag ataaaaaagt attttcactg caagcatagg atatacgaat atatttatcc
      301 ataccaaatg ctatgccagg aacaactgca acattatatt cttctaaaaa ttgattacaa
      361 aattctatag aaaaactatc tttatattca aatttctcag atactttaga aagatctata
      421 aatatataga atgcgccatt tggattcaca tatcctacat tatcaataga gtctagtttt
      481 gatttaatta aatctcttct tgatttatat gttttaacca tattgtctat gtcaattgag
      541 caatctttca aggctccata ggctatgtat tgtgctgtta gacttgggtg agatataaga
      601 tgtccttgta tagaactcat tgctttagca atcgttttat tagatgcagt atatcctaat
      661 cttaatccag tcattgcagc tgacttagaa aaaccattta tagttattgt tatatcttta
      721 gcttcttcgc ttaatgatgc aattgatgta aattcaccag tgtagcatat tttttcatat
      781 atttcatctg ctaaaatata tattttattt tgtatgcata catctactat ttctattaat
      841 tcatcttttg tataaacact accagtagga tttgaaggat tgtttattac aagaattttt
      901 gttttatctg ttatagattt ttcaagttct tcttttgtta acttaaatcc attttctttt
      961 ttagtatcaa taaaaactgg aacagcatta actaacttaa tcatttcagg ataactaacc
     1021 caataaggct ttggtaataa gacttcatct ccttcgtctg ttaaagctag taaagtattt
     1081 gttattgagt tttttgctcc acttgataca actatttcgt ctattgaata gttacagtta
     1141 ttttcttcaa taagtttttt acagatttcc tctctaagta tttttaatcc tggaactaaa
     1201 tcatatttag tgtaatcttt atttaaagaa tcaataccat aagattttgc gttattaggt
     1261 acattaaaat cgggttctcc tatactaaga tttattactt taactccatt actttccatt
     1321 tctttaactt tagaactaat acctattgtg taggatggtg ttataaagtt tagtcttttt
     1381 gataacataa aatgcctcct tttaaattat tatttatatt atacgacaaa tttaaagaat
     1441 ttacataagg aaatttgtag atatatacat taatatatat aatagcctac actttttgcc
     1501 taaaattagt gtaaaaaaaa gagaaaaaaa ttttaacttt caaagttgtt agaaacgcta
     1561 ctgttcaaaa tatccacata gtatttgcaa tgatttgcgc aagtttgact attatgtaca
     1621 aatttggtaa tcttaatata gcaattatta agataatccc tttaacttag ttgaagggga
     1681 tttttttaga cttaacatac tacatatagt ggtattggga ttaataaaat acaatatata
     1741 gtaacaggag gtttttttaa ttggttgaat tcgtaaaaaa aagagatgga agagtaattc
     1801 catttaatga agatagaata acaagagcta tatttttagc agcaacgaat gttgcagaaa
     1861 gagaaggaat agtacctgat tataaattat ctgagcaatt aactcaagaa gtaataaagt
     1921 ttttaaatca taaatactca gaatcagttc caagtgttga agatatacaa gattctgtag
     1981 ttaaggttct tatagaaact ggccatgcaa aaactagtga agaatatata atatacagaa
     2041 ctgaaagaag tagaataaga aattcaaaaa caagattgat gaaggcaata gaggaaatta
     2101 cctttgaaga tgcagaagat gctgatataa aaagagaaaa tgcaaatatt aatggtaata
     2161 cagcaatggg aactatgtta caatatggaa gcactgtatc taaagaattt tgtaaaacac
     2221 atatattaaa accagaacat tcttttgctc atgataatgg agatatacac atacatgata
     2281 tggatttttt aaatatggga acattaactt gttgtcaaat agatgttaaa aaactattca
     2341 atggtggatt ttcaacagga catggctttt taagagagcc acaagatata ataagttatg
     2401 gtgcattagc tgcaattgct atacaaagca atcaaaacga tcaacatgga ggtcaaagta
     2461 taccattttt cgattatgga ttggctgaag gtgtatataa gacatttaag aaattctaca
     2521 taggaaattt agctaaagca ttgaagttat ttaaaggaat tgaaaataat gatgtgataa
     2581 agaacatagt atataataca gagaaagaaa caaatcaaaa agttggtctt aagagagatg
     2641 aattgtatct aaacttagaa aaagagaaat taatacagac ttttgatata gatgatgaac
     2701 ttgtaaataa gatgcaaaac tttgcatttg aagaatcgta tagagaaact gataaaaaaa
     2761 catatcaatc tatggaagca tttatacaca atctaaatac aatgcactct agagcaggtg
     2821 cacaggtacc attttcaagt gtaaacttcg gaacagatac ttctgaagaa ggaagaatgg
     2881 taactaagaa tcttctatta tctcaagaaa gaggtttagg taatggagag acaccaattt
     2941 ttccaatttt aatatttaag gtcaaagaag gcataaattt aaatcctgaa gaccctaatt
     3001 atgatttatt taaattatca tgcagagtat cagctaaaag actatttcct aattttagtt
     3061 ttttagatgc gccttttaat gcaaaatatt ataaaaaagg tgaaccagat acagaggcta
     3121 catatatggg ttgtagaaca agagtactta gtaatgtgtg tggttcagaa acagttagtg
     3181 gtagaggaaa catatctttt actacagtaa atttaccaag attgggtata aaacatggta
     3241 ttataaataa tgaaaaggct aatttagatg gtttctttga agaattagat gagaagataa
     3301 acttaattat agaacaatta ttggagcgct ttgaggttca aggaaataaa aagatgaaga
     3361 attttccatt cttaatgggg caaggtgtat ggaaaggttc tgatgattta gtaccagaag
     3421 atacactaaa agaagtaata aaacaaggaa cattgacaat tggatttata ggtttagcag
     3481 agtgccttat tgctcttata ggtaaacatc atggagaaag taaagaagct caagaattag
     3541 gattaaaaat agtgtcacat atgagacata aaatggatga agcaacggac aaatataaat
     3601 taaatttctc cttaatgggt actccagcag aaggtctttc aggtaggttt actaaaatag
     3661 ataaaaaagt ttatggagaa attaaaggta tcacagataa agaatattat actaattcat
     3721 tccatgtacc agtttattac aatataagtg catatgataa aattgagata gaagctccat
     3781 accatgaatt gactaatgca ggtcatataa catatgtaga gttagatgga gatccatctg
     3841 ataatttaga ggcttttgaa actgttataa aagctatgaa agatttgggt ataggatatg
     3901 gaagtataaa tcatcctgta gatagagacc caatatgcgg tttttcaggt gtaataacaa
     3961 gtaatatatg ccctgtatgt ggaagaaatg aagatgaaag tgacatcaaa ttcgaaagaa
     4021 taagaagaat aacaggatac ttagttggta cagttgatag atttaataat gctaaaaaag
     4081 cagaagtaag agatagagta aaacatagat agttttattt gggggaataa gttatgaaaa
     4141 taagaatgtc atctactata agttatgaca gcatagttga tggaccagga ttaagaatgg
     4201 taatatggac tcaaggatgt attcataatt gtaaagagtg ccataatcct cagacacatg
     4261 atttatgtgg aggattttat atggatacag aagaaattat aaataaagtc aaatcattga
     4321 aattacaaaa aggcattacg ctatcaggag gagaaccatt tttacaacca gaaccattag
     4381 aagaaatagc aagagaagct aaaataaatg ggttagatgt atggtcatat actggattta
     4441 cgtttgaaca actgttagat aaaaaaaata gagcgtattt taaaaattta aatttactaa
     4501 agcaaattga tattttagtg gatggaaaat ttatagctga aaaaaaagat ataagcttaa
     4561 agtttagagg gtcatcaaat caaaggataa ttgatgttca aaaaagtttg aaatataaaa
     4621 aagtgttttt ggttgagcaa tatatgaagg atgatttatc tatagca
//
LOCUS       DAEPOZ010000253         4620 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.253, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000253 DAEPOZ010000000
VERSION     DAEPOZ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.253"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            232..1149
                     /locus_tag="KR440_003262"
     CDS             232..1149
                     /locus_tag="KR440_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBH0700268.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     ICSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVNQ
                     GEELIELSKKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVRFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKHFSVNTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAINGEECIKAQRLLEKIINSVK"
     gene            complement(1278..2207)
                     /gene="pfkB"
                     /locus_tag="KR440_003263"
     CDS             complement(1278..2207)
                     /gene="pfkB"
                     /locus_tag="KR440_003263"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBH0700269.1"
                     /translation="MITVITFNPSIDRMYKVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGINIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEYNVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLGEIDKYQDLVEVKKLS"
     gene            2476..3201
                     /locus_tag="KR440_003264"
     CDS             2476..3201
                     /locus_tag="KR440_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0700270.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRIQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            3284..4438
                     /gene="nagA"
                     /locus_tag="KR440_003265"
     CDS             3284..4438
                     /gene="nagA"
                     /locus_tag="KR440_003265"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBH0700271.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNITDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQRKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFSVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNIATLEDAIKM
                     ATYVPALSCNIDNVCGSIHQGMDADFIVTDKNFNIYETYISGICMYKVNK"
BASE COUNT         1724 a          618 c          660 g         1618 t
ORIGIN      
        1 tggtaattac cactaatcca aatataaatt aatttgaatt ttttgtcaat acataaaata
       61 tttatatcaa tcaattttat agatatccta caaaattact ttgtcaatat ttcttatttc
      121 gttaatttac ttatataagc tttaaattta ttttttgata caaagtttac aaatttatgt
      181 gtctgaatta taatcaattc tgtaaatata aaaaccttag gagaaagtat tatgaaaaat
      241 ataaaaatgg gaatgatagg tcttggctcc atagctcaaa aggcatattt acctatatta
      301 actaagtctg agagatttga gtttgtagga gcttttactc caaataaagt taaaagggaa
      361 aaaatctgta gtgattatag aattatgcca ttcgattcta ttgaatccct agctaaaaaa
      421 tgtgattgta tttttctaca tagctctaca gaaacacact acgaaattat aaaaatatta
      481 ttaaatttag gtgttcatgt atatgtagac aaacctcttg cttcaacagt aaatcaagga
      541 gaagaactta tcgagttaag taagaaaaaa aacttaaatt taatggttgg attcaaccgt
      601 agattctgtc ctatgtacaa agaaattaaa aataatgcta cagaaatcgt atctatcaat
      661 atatgtaaac atggattaaa ttccttaaga aatgttagat tcgacagtac ccttatagat
      721 gactatatac atgttattga cactgcttta tggttagcaa atgaagatgt tgaaattagc
      781 ggagaagatt tatttttaac agacaataag aatcttatat ttgtaagtca taaacttaaa
      841 ggtaaacatt tctctgtaaa cacctctatg catagagatt ctggaacaaa gttagaacaa
      901 gttgaaattc tttcaaaagg taagattcaa agagttaaga atcttaatgt actggaaata
      961 gaagaaggtg gaaacctaac tttaaaacaa agtggtgcat gggtaaatat attaaaacaa
     1021 aagggttttg aggatatatc aaatcatttt attgattgta tagaaaataa tataaaacca
     1081 gctattaatg gagaagagtg tatcaaggct caaagattac tagagaaaat tataaattca
     1141 gtaaaataga tatttattat aaaaataggt taaaaatgaa tttttctaac ctatttttat
     1201 ataagactat atcttttata ttaaaattaa aatctattac aacaacatta aatttattat
     1261 attcccatta aatttattta acttaacttt tttacttcaa ctaaatcttg atatttatca
     1321 atttctccca aatctacaaa tccagtttcc atatttaaag cattacttat accgcaagca
     1381 tttgcaaatt taagtaaatt ttctatttta taacccctta aaatacctac actaaatcca
     1441 gccactgtgc tatctccact acctacagta tttatacact ttacaactgg tacttttaca
     1501 tcatatacat tatattcgtt taaatacacc ataccatctt ttcctaaaga tatacataca
     1561 ttctctgcac ccatttcaat aagttttttt cctgacaaga taatttcctc tcttgattct
     1621 atatttattc ctaataaatg tcttatttca tctatatttg gctttattaa atatggtttt
     1681 gattttaaag ctatttttaa tgcttctcct gatgtatcta atataaattt tatattattt
     1741 gctctacata tatttcctat cttttcataa taatctatcg gcatattctc acaataacta
     1801 ccagaagcta ctagtatttt agtatttttt agtacttcct ctatatcatt ttcaaatctc
     1861 tctaaatcgc ttttttctac tattggtcct ttttctaaaa attctgtact aactttattg
     1921 tcctcaatta tgtttaagca atttctagtt tcctgctcta tttttgtaaa tttattttgt
     1981 atatctagtt ttctaagttc attttttata aattctccat taaatccacc tagaaatccc
     2041 atagctaaag gattttcttt taaaatttta cataccttag ctacgtttaa tcctttacct
     2101 ccagcagttg cattcttact tactaccctt tgcacctcac ctatgtttat attattcact
     2161 ttatacattc tatctataga tgggttgaat gttattactg taatcatact agattctcct
     2221 cattattaat ttttactttt ctaaacaata aattaatctg taaaataaaa aatacctcat
     2281 ctataatata cccaaagtgg ttattaccaa tttgaatata aattatttta cttgttttgt
     2341 caatatacca taattttata ataaattaat agccttactt attgaatttt gattaaactt
     2401 tattcattga cataaataat tctatattct ataatcaaat tgtggtaata accacttaga
     2461 aaggactatg tttttatgat aaaaagaaat gataaaagac caatatatga ccagcttgtt
     2521 gagatattaa gaactaagat tgaaaatgag atggaaccaa acgatagaat gctttctgaa
     2581 agaaaaattt gtgatgaata tggagtaagt agaaccacag ttagactagc tatggctgag
     2641 ttggaacata tgggatatat atacaaaaga catggaaaag gaacttttgt tgctgcacta
     2701 agtcaaaatt ctcaaaactt aatggaaagt tatagcttta cagattatat gagaatacaa
     2761 ggaaaaaagc caggtacaaa ggtattatca tttgaggtag tagaaagtac aaagtatttc
     2821 tctgaaaaat taggtataac tcctggtgaa aaaatgataa aaataatgcg tttaagactt
     2881 gctgatgacc ttcctatgat gcttgaaaga acatatcttc ctatgaaaga atttatagga
     2941 ttgacaaaag aaaaaatcga acaaaaacct ttatatgaaa tatttagaga agattatggc
     3001 gagattatta aagttgctga tgaagaattt tcagctggaa taatctctga taaggaagct
     3061 aagttattgg aagtaccagg agattcacct tgtctgaaat tacttcgtac cacatataat
     3121 gatgacaata tagttattga atttactttg agtgttgcta gaagcgataa atttgtatat
     3181 agagtaagac atgtaagata gtgaattaaa tagctttcaa gaaaatagaa tgatattata
     3241 aacgtcaatg ccacaatgat agtataatta taaggaggtt attttgaaaa gttttattta
     3301 tgcagataaa ttttttatga aacatggaat caaagaaaaa ggttacctta gtataattaa
     3361 tggcaaattc ggtgaatttc aacaagaaag acctaatatt actgataatg tcatagattt
     3421 ttcaggtaaa tatattgctc ctggcttagt tgatactcac attcacggtc ttttaggtgc
     3481 agatgtcatg gacaatacat ttgaagcaat aaacacaatt tcaaaaggct tattaaaata
     3541 tggagtaaca tctttcctac ctacaacttt aactgattct gtagatacac taagtgattc
     3601 tgtagaaaat ataagcaata gcttcaatcg agttgagggt gcaaaaatac aagggattta
     3661 tttagaaggt ccatttttta cagaaaaata taagggcgct caaaatgaaa aatattttaa
     3721 agaccctgac atcaatatat taaaattatg gcaaagaaaa tcgaaaaatt taataagaaa
     3781 aatagcaatt gctccagaac gtaaaggttc aatagaatta acaagatatg caactaaaaa
     3841 taatatctct gtttctcttg gccatagttc agctacattt gaacaagtta aacaagtcgt
     3901 taattcaggt gctaaagtgt ttgtacatac atataatggg atgagccctt taaatcatag
     3961 agaacctgga atggttgggg ctgctatggt actaaaagat acttatgctg aattaatttg
     4021 tgatggtcat catgtaagcc caatagctgt taaaataatg atggatgcga agtctagtaa
     4081 taacatagct ttaattacag attgtatgag ggctggtgct atggaggatg gaaaatatac
     4141 tcttggtaag tttagtgtaa atgtaaaaaa tagtgttgca agattaaata gtgggagttt
     4201 agctggaagt gtattaacta tggacaaggc tgtgagaaat atagttgatt ggaacatagc
     4261 cactttagaa gatgcaataa aaatggctac atatgttcct gctttaagtt gtaatattga
     4321 taatgtctgt ggctccatac atcaaggtat ggatgcagac tttatagtaa cagataagaa
     4381 ctttaatata tacgaaactt atataagtgg tatatgtatg tacaaagtta ataaatagat
     4441 tcaatataaa gttatggagg cttcctaaaa atagagataa gtctcttttt cattgttaaa
     4501 atgatgaaaa tacatttgat ttcatacaaa aagagtgcct cataaactaa aaagttaatt
     4561 ttgagacact ccttttatta aggttcttta ttttacaata taagtttata aaattaagaa
//
LOCUS       DAEPOZ010000254         4619 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.254, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000254 DAEPOZ010000000
VERSION     DAEPOZ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.254"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(169..738)
                     /locus_tag="KR440_003266"
     CDS             complement(169..738)
                     /locus_tag="KR440_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBH0700272.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
     gene            complement(810..1418)
                     /locus_tag="KR440_003267"
     CDS             complement(810..1418)
                     /locus_tag="KR440_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBH0700273.1"
                     /translation="MDSNDKKGLKSTEIIIINGSPRINKNCSFIANKVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRNKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            complement(1426..3189)
                     /locus_tag="KR440_003268"
     CDS             complement(1426..3189)
                     /locus_tag="KR440_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HBH0700274.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDTIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNETIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMKDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            complement(3232..3699)
                     /locus_tag="KR440_003269"
     CDS             complement(3232..3699)
                     /locus_tag="KR440_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700275.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLAFGSILLMIAFNLNMILGIGVFLIGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            3882..4343
                     /locus_tag="KR440_003270"
     CDS             3882..4343
                     /locus_tag="KR440_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700276.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQHFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKA"
     gene            complement(4436..4606)
                     /locus_tag="KR440_003271"
     CDS             complement(4436..4606)
                     /locus_tag="KR440_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700277.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
BASE COUNT         1578 a          765 c          427 g         1849 t
ORIGIN      
        1 attttaccat attacatcat atttttgtaa gtttttatta cattttttat aatacccctt
       61 tattttataa attaatctaa tcgattaaat ttatcctata gaaatttatt acatttaaaa
      121 atatgctagc aaataaaata tctactagca taaagtctat ttataatatt aatcaactat
      181 taattctact aaatctccat ttttaacgcc tgctgcattt ccctcttcca tatcaacatg
      241 catttctaga ttaaagctat cactagctct tactaataca ttttcaaata ctaatgctct
      301 tggtccagat gttttaactt ttactatttg cttatcagtt acaccaaatt tagcagcatc
      361 ttcattgctc atatgtatat gtctagaagc aactataact ccttctttca tttcaacttc
      421 tccagcaggt cctactaatt taacacctgg agttccagct atatcaccag attcttttat
      481 tggtgctttt actcctaatg ggaaaccatc tgctaaagat atttcaactt gagtatgagc
      541 tctagctggt cctagtactc taactccctt taaagttcct tttggtccta ctaaatccac
      601 tttttcttca caggcatatt gccctggttg agataatggt ttaaatttag ttaactcatg
      661 acccgcccca aataatacgt ctatatcagc ttgactcaaa tgaacatgtt tattagataa
      721 tgctattggt aatttcattg aaattccctc cttttaatta tttaaattat tgcaatacaa
      781 ttatacataa tattacctac aaaatcaaat tatattgaat caatatatat ctttaaattt
      841 ttatatatta tatctttaaa ttcttgatta tctatataag atacatcatc tgtattgttt
      901 aaatatatat tgtgtaaaag tttagtattt atactcttaa agaaaaaatc cataacaatc
      961 tgtccacctt taaactgtga atcatatgat ttactccccc ccactgcaat atacattcct
     1021 atccgtttct tatttctatc aattgaaggt ttatttaata catatttact tgcatatata
     1081 gcttgagttc tatcaacaac agtctttaat ttagctgata ttgaatcaaa atgtacagga
     1141 cttattacta tagttccttt tgaatcatca aacattttat ataatggctt catatcatca
     1201 ttaaacttgc aataccctgt tttttcacaa aatccacatg ctgtgcaata ttcaatatcc
     1261 atattgtaga tattaaatat tttacaactt aacccacact cttctagcat agcttttact
     1321 ttatttgcta taaaactgca atttttatta attcttggac ttccatttat tattataatc
     1381 tctgtacttt taagtccttt tttatcgttt gaatccataa tcaatctacc tatcctttaa
     1441 cataactatt aaaatatcat catcataatt atcttttgat gagaaattct ttaaatccaa
     1501 ttttagattt tctactatta actgttgatt taatctaaaa ttttctttta gaaagttttc
     1561 taatctgttt ataccatact cttcttttga tgaatttttt atctctaaaa ttccatcagt
     1621 gtacatacat accattgcat aatcttccat tgaaagagta ttattttcat aagtagcatc
     1681 tttcattatt ccaataggta tgcccttttt acaatttaaa ttattttcta ttgtgccatc
     1741 tttctttaca actacaggac tatagtgacc tgcattagac acagtaatca tattttcttt
     1801 agtatctata atacctatca aacaagttgt aaatactccc atcttgtcaa attcatcata
     1861 taacatccta tttaaatttg tcattatttc tcttggtgta ttgtattgat tgcataatac
     1921 tttaaaagca ccttttatca ttgcaacaac ataatttgat accattccat gtcccattac
     1981 atcagcaaca atataaacta tatgttcgtc atccactttt gtagcataat aaaaatctcc
     2041 acctactacc cttgctggtt tatggtaaaa ataaagttct ttctcatcat tacaatccat
     2101 tttaccttta tccataataa gctgttgttg tttatttaat atgttcaact cattttcaag
     2161 aactttgtgt tttacttcct tttcattatg ttcatataat tgcatcccaa cagaaacttg
     2221 tttggacaat atactaagaa tatttaaatc atcactagta tagtttttac tatttgcaca
     2281 aactatacat cctatagtct catttttatc ctgaagtttg taatatatat aactttccaa
     2341 atttatgccc ttttttgagc atggttttaa attatttagg tatcccccta atgactcttt
     2401 ctttaaaaac atatcaatca taggatataa atttgttttc tttttttgct caacattata
     2461 acaactaact atagtatcct tattatgatg actatttata attattaaag cttctttact
     2521 attagtaata ttacacgctt gtttacttaa attttccaaa aagccatcaa tactcttatt
     2581 aaggtatagt tttttagtag cttcatttat ggcacttatt gagcctctta gtttatcaat
     2641 taaaatttgt acattattat ttttattagt tatttctatt gataaactta agagtgatgc
     2701 aacagatgat ataaaatcta tatcttgttc tgttatataa tcatcctctt ttaaaaaaca
     2761 agtcataaat ccaacaacta cattatttat tataaaagga aaaactattc tcccaatata
     2821 accttcagct tttgctaaat ctcgttcacc ttctgctcta gaatcttcaa atacattttc
     2881 cacatatatt atcttttttt catttacagc ctcatgtata tactctggat atgtgtcaaa
     2941 atcaattttt gcacctttaa gactatttat tggaaaaatt tgaggtacat attctaaggt
     3001 ttgcgaacat actaagtagg catatttaga atcattttta taaaatagat ttacacatgc
     3061 ctttgtaggg tgaacaactt ctaacatttt ttctattatg tcatctttta tattaaaaaa
     3121 atctgtagat tccgtcacca tagcggatat ctccactaaa ttcttcattt tataactgcc
     3181 attagtcata ctcaatttct ccccctatat attaaactat ttattagtat tttaacatac
     3241 attctttata gtaaaaacaa aaacaacgac aaaactagca actaatatcc agttaaatcc
     3301 tgtcaataat cctataagaa gtaatgtaat caatgcattt agacctgttc tccctttttc
     3361 atccataaaa gttattttag agatgtcgat attatttttc acattattta tatgctcttt
     3421 tacttgttct tccatatttc ttttatatag tatataaact acaccaatta aaaacactcc
     3481 tatacctaga atcatgttta aattaaaagc tatcataagc agtattgaac caaaagccaa
     3541 ctttaaaagc ttaaaaaata cgctatctat ctttagagtt atatttctga tttctttaca
     3601 ttcatctctt aaagaatatc cacttgagtc taggttatta atctttgttt ttacaatttc
     3661 agaataaaaa agctttatta tcccatttaa cattttcatc actcctaaat attttacata
     3721 tatttgtaag ttttacaatt ataatatttt ttatacttgg taataataaa aaataacaaa
     3781 tattttatga tttagtattt actgtaaata aattattgaa gctttattag ttaagtatag
     3841 atttttaata aatattttta taattagaag gaagtggttt tgtgcaaata gaacaactcg
     3901 atttagaaac gagaaataaa atatactgct atactaaaaa aattcttaga aaatatcaaa
     3961 aaggcataac atctggaaaa ttaacagcag ataaatttgc agataatata ttgtctcaac
     4021 attttatcag tagtatactt aatgaaaaaa tagtcaatga gacaaatttt aagatatctt
     4081 atagaaacta tatagaaact ttaataaata ttcaaaatga aaatttatct aacttaagaa
     4141 aaaaatctac taaaactgca aaatgtttca atatctctca aataacccaa ttaaaaaact
     4201 tactaagtaa tactggttat aatttattaa ttccatataa atacttaact gctagagata
     4261 tagaaggaat cgttactctt attaatactg ggtctattga acttggaaat gagagaatct
     4321 ataattatat atcaaaagca taataattta aacctattat aaaaaggcaa tttatttaat
     4381 aaaaaatatt tttaatatta aataaattgc ctttttaatt ttggactaaa tactgctatc
     4441 caagtgcttt ttctagttta gataaatgtt gtattggata atattttagt agctctttaa
     4501 attgttctac tgataaacct tctgttgttg tatatattct tattttttca tctaagtcac
     4561 tattaataaa tttgtttttt aaatcctcaa aatttacact actcatttta catcactcc
//
LOCUS       DAEPOZ010000255         4561 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.255, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000255 DAEPOZ010000000
VERSION     DAEPOZ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.255"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(451..2838)
                     /gene="cadA"
                     /locus_tag="KR440_003272"
     CDS             complement(451..2838)
                     /gene="cadA"
                     /locus_tag="KR440_003272"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBH0700278.1"
                     /translation="MEANNLIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLIVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENASSKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFKEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELVPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALTIGESSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKFNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIRSLKAIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
     gene            complement(2861..3226)
                     /locus_tag="KR440_003273"
     CDS             complement(2861..3226)
                     /locus_tag="KR440_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0700279.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            3425..4273
                     /locus_tag="KR440_003274"
     CDS             3425..4273
                     /locus_tag="KR440_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700280.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMSNNMNSNMGMPNNMPNNMNSNM
                     GMPNNMPNNMNSNMGMPNNMPNNMNSNMGMPNNVPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSIQGIPQATNIEE
                     FDEEEM"
BASE COUNT         1539 a          803 c          502 g         1717 t
ORIGIN      
        1 actatatcac cttaaaaata tttcgtcaat acttttttaa attatttttt aaaaatattt
       61 actttcttat attacataat ttattgaatg acagtcatac tgttggttta gagtatttta
      121 acacacacat tttgttgtaa aaattataac tattaatata atatatatac aatcataagt
      181 attataattt ttatgaatct aatttaaact taatatagac ttattaataa accttctatg
      241 tctaaatttt aaaattacaa tataaaagct ctacactaaa taaatacatt aaataaacac
      301 aatggataat gaatataaat ttatatatgc aaagcgctcc aataaaaagg taccaataaa
      361 gttaaaagaa acttattgat acctttcatt attacatact tcaatatata attataagtt
      421 ttttaaatag tatggattta catttatccc ttatctcata gctctcatag catttactac
      481 tgctataagt gccacaccta catcagcaaa tatagcttcc cacatagtag ctactcctcc
      541 agcacctagt atcataacta taatttttac tcctagagca aatattatgt tttgccacac
      601 tatcttatta gtcttgtttg ctatttcaat tgctttagat atcttgctag gctcatctgt
      661 cataagcact acatctgcag cttcaatagc agcatctgaa cctaagcctc ccatagctat
      721 accaacatca gcacgagcta atactggagc atcatttatt ccatcaccta caaatgctat
      781 tttctctttt tcagttcttc cttcatataa atcttctaat ctatctacct tttcattagg
      841 aagtaaattt gagtaaactg tatccaattc taattcttga gcaatatttt ttgctacttt
      901 ctcattatct ccagttaaca ttacaacttc ttttacccct attgctttta agcttcttat
      961 ggcattttta ctatcttctt ttacttcatc agatataact atataacctc tatatacttt
     1021 atcaacagca atatagacta ctgtgcctac ttctttagct gatgaataag atatattttc
     1081 ttttttcata agtttctcat tacctgctaa tataaaattg ccattatgct ttactctaat
     1141 tccataagct gctatttcct cataactgtc tattgtatct aaatcaattt ttttattata
     1201 gtaacttaat atagactttg ctattggatg atttgaattt acctccgcaa tagctgcata
     1261 ttctaataat tcttcttctg atataccttc aggatttaac ttagttacat tgaacacacc
     1321 tttagtaagt gttcctgttt tatcaaatac tactgtatct acacttctta aagcttctaa
     1381 atagttactt cctttaataa ggataccatt tttagatgca aacccaattc cactaaagaa
     1441 actaagtggt attgataaaa ctaatgcaca agggcaagaa acgactaaga atatcaatcc
     1501 tctatataac caatcactaa atacctcacc tgagattaca agtggtggca caaatgctat
     1561 taataaagca gctattacta ctattggagt atagtatctt gaaaacttag atataaagtt
     1621 ttctgtcttt gattttttaa tgctagaatt ttcaacaagg tcaagaatct tagaaactgt
     1681 tgattctcca aaactctttg taacttcaat actcaataaa gcatttttat ttataacacc
     1741 tgataagatt tcatctcctt tttcaacttc tctcagcact gactctcctg tgagtgcaga
     1801 agtatctagc atagaaactc catccactac tacaccatct aatggtactt tttcaccagg
     1861 ttttactact attatatccc ctacatttac ttcttctggt gacactacct taacttcaga
     1921 attaaatttt agatttgcat aatctggtct tatttgcata agtgaagtta tagactttct
     1981 ggatttacca actgcaacac cttgtaaata ctctcctact ttatagaaaa gcataacccc
     2041 tactgcttct gatgactccc ctattgttaa tgctcctact gttgctagag ccatcaaaaa
     2101 gttctcatca aatactcttc cttttgatat atttcttatt gctcttaaaa gcacatcgcc
     2161 acctactatt acataggcaa ctataaatac tatattgcta aactgacttt cataacctgt
     2221 agctgtttga tatataccaa atatatatac aagtgctcca attataagtg gaactaattc
     2281 tttcttattt gtatcattta cagctcctga tttttcagta gttctccctt ttactttgtc
     2341 agtagaccct acttgtatat caagtcctgg ctctgttgaa tttattattt cttttatttt
     2401 atttataaca tcatcctctt taaagttatt acttatattt actgtaagtt ttttatttac
     2461 aaagtttaaa ttagaagatt caacttcttt taatttagaa accttgttat taatctcctc
     2521 tgcacaatgt gcacagttta atcctcctaa tataagctct tttttaattg atgttttact
     2581 agatgcattt tctttagaac ttatttgtat atcaagacct ggttctgttg aatctattat
     2641 atctattatc ttttctatag tagtatcctc attaaagctt tcctttatat ttactataag
     2701 ttttttattt ataaaattta agttggaaga tttaacttct tgtagtttag aaactttatt
     2761 attaatctcc tctgcacaat gtgcacagtt taatcctcct agtataaatt cttttttaat
     2821 taaattattt gcttccatat atattcactc ctctcattta tcatttataa gtttctctaa
     2881 tatgatttaa tccacaatca aatatctggc ttatatgact atcatctaat gagtaataca
     2941 ctgtttttcc ttctcttcta aactttacta atctagcctg ctttagtact ctaagttgat
     3001 gagatattgc agagtgagtc atatttaata aactcgctat atcacataca cacatttcat
     3061 ttgcaaataa tgcatataat atctttactc tagtagtatc cccaaatact ttaaataatt
     3121 ctgctaaatc atataacatc tcttcatctg gcatagttga tttagcctct gttactattt
     3181 cttcatgtac tatattacaa ttacaatcat ttatctcttc tctcatcttt aatcctccat
     3241 tgttttaata tatgaataac tgttcatatg ttcattatat gctatcattc ctaaatttgc
     3301 aacaataatt tttattaatt taatataatt agaatgcaca tattataaac ttttttaata
     3361 tatatattat tagaataagt ttttgtaaac ttttttatag ttttctagga ggtgcaagtt
     3421 taatatgagt aacaagaaaa aaaaggattt agatactagt tacatgcctt acttagaccc
     3481 agcgaactat gcagattata ttactaatga agaaattccc caagatgaat tgagaaatga
     3541 ccctcaaatt tctaatggtt atacaaaaat acctaataac cagaacataa ataacctata
     3601 tcctaataat gtaaatcatt atcctaaaag tagtaattct ccaaacaact tgcctaacac
     3661 aaataataca tccggaaata tgaattacaa tacatctgct acaaataata tgcctaataa
     3721 catgaataat aatacgggta tgcctaataa tatgtctaac aatatgaata gtaatatggg
     3781 tatgcctaac aatatgccta ataacatgaa tagtaacatg ggtatgccta acaatatgtc
     3841 taacaatatg aatagtaata tgggtatgcc taacaatatg cctaataaca tgaatagtaa
     3901 catgggtatg cctaacaata tgcctaataa tatgaatagt aacatgggta tgcctaacaa
     3961 tatgcctaat aatatgaata gtaacatggg tatgcctaac aatgtgccta ataacatgaa
     4021 cagtaatatg gggtataata tgaataatac tatgggtatt cctaacttct catgtaatca
     4081 aaatatgcct cctaatgtac ttatgatgat accaggagtt atatgtcata atacaatgca
     4141 aggtatgcct gtagttatgc ctagtactat gcctcctaat atatatccta ctccttatgg
     4201 ttctagtaat atgtctatac aaggtatacc acaggctact aatattgaag aatttgatga
     4261 ggaagaaatg taatatatat ttttttgagc aagttgtata aaacattaat aattgaccat
     4321 aataataaat gtattcctca taatattccc ccaatattat tataggtata gaaagttgac
     4381 tgagtatgcg cttattcggt caacagatat taacataatc taataagata aacatagaaa
     4441 aagtcagata aaatttaatt ttatctgact ttttcattta aaaaactctt tcttgtgttg
     4501 gtgggtcaga atagtatggt actcgtattt gagactaata ttggcaaaca acataaagaa
     4561 a
//
LOCUS       DAEPOZ010000256         4505 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.256, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000256 DAEPOZ010000000
VERSION     DAEPOZ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.256"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            141..1442
                     /locus_tag="KR440_003275"
     CDS             141..1442
                     /locus_tag="KR440_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0700281.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            1518..1793
                     /locus_tag="KR440_003276"
     CDS             1518..1793
                     /locus_tag="KR440_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBH0700282.1"
                     /translation="MLELTKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            3118..3348
                     /locus_tag="KR440_003277"
     CDS             3118..3348
                     /locus_tag="KR440_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0700283.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            3341..3922
                     /locus_tag="KR440_003278"
     CDS             3341..3922
                     /locus_tag="KR440_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBH0700284.1"
                     /translation="MNNKKHWYDYLWIFSSIYLVLGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSV"
BASE COUNT         1747 a          367 c          883 g         1508 t
ORIGIN      
        1 atattaataa tttgtagtat atcatgacga ataatatagt cataacaaat agtgagtcat
       61 aacaaataat gtatttataa ttgataatgt atttaaaaat caaaatataa aaacttataa
      121 ggaaagagag attaatttat atgggtaaat caaaagatgt tgaaatagag aagtctattt
      181 taggcacaat tcttttagac aataaattaa gctataagct agatgaatta aatgaaaaca
      241 tgtttatgaa tgatatatgt cttgagatat ttaaaattat gaaagaactt aaaaaagaaa
      301 acatagtgat tgatgtggcc actgtgaaat caaagataga tagaaaatct ctagcaataa
      361 aaacgagtga tgtgactaat ctaattactt ggggtcagaa ttttggtctt gatggtcata
      421 ttaaaatttt aaaagaaaat ttagctagaa gaagcataaa tcaaaactgc caaaaccttt
      481 tacacagcct aaaccttgga gaaaatatcg atacttgtat ttacaaattt gaaagtaata
      541 taaaggagat tttggacaag gatacttatg aaaatgatga tgtaaatagt atagcaggga
      601 aggtgcttga ttttttagaa aacaaaaaag atattggatt taaatttggt ataaaattac
      661 ttgatactac tataggaggt ctttttaaag gagagctaac cacaatagca gctaaatcgg
      721 gggttgggaa aactgctttg gcacttcaaa taatgttaaa ttcatttaag caaggtaaaa
      781 aaactttatt tataagtaga gaaatgacca gtgagcaagt atttatgaga aatatatgca
      841 gagtaactgg tgtaagtaca aggaatatga aatctaaaga aatagatgaa aatgattgga
      901 aacttattgt aaatgctata ggtgatttaa gcgaaaataa cttaatttat attaatgata
      961 agatagatac aattagtgca attagaaaga ggataaggca ggtaaagcca gatttattaa
     1021 tagtggatta tgtgcaactc cttacatctc agaaaagcat ggataagaga gaaagggagg
     1081 tagctacttt ttcgagagaa cttaaaaata tgactttaga ttttaatata cctgtaatcc
     1141 aattaagtca gttaaatgat gaaatgaaag attcaagacc ttatggagat agacctatga
     1201 gggatagcaa agccatctac cacgatagca acaatgtggt ttatatacat caattaaagg
     1261 ggagtgatta tgaagaagca gtgagagata ttggagaaag tgaggaggca gttagagcat
     1321 cagaatacag gggaattaag atggttgatt taatagttgc gaagtgtaga gatggacaga
     1381 ctaggcataa gcatttttgt tactttgggg ataagttaca ttttcaagaa ctaaattatt
     1441 aggtcaataa agtagtaata acaagcttat taaaagtcag gaaatagcaa atagattata
     1501 aaatatgagg tgaaaatatg ttggaattaa cgaaaattat agaattactt gaagaacaca
     1561 agatagaagt aagtattcca aaggagttac ataagtatag tttattggaa taccttgaat
     1621 tgtgtcaaca aagtggggga acatattttt atattaaaac actggaaggg gcattaaaag
     1681 aggctagaaa tgagcttatg attaaattgt ttaatgggaa aaattatatg gaattagcga
     1741 ggatgtttga aatgagtgtt attaatgtca ggcatattgt tagaaaaaaa tagatatttt
     1801 tggagaatac aagtgtgata tacttctgaa atagaagtta ttatagttga aataaaattg
     1861 tggctaattt gtgtataagt ttaagattat agaggagtat aagtgtgatt aacagcatga
     1921 ttttgtgaat aaaattgatg atatatatga gttaaatgtg tgattaataa ggtaaggttg
     1981 tgtataagat tatgagtatt taaaaggagg ggctataatt aatagtgtag agttgtatgt
     2041 ttgcttaaga ttatataata atttaataga ttaaactata cgaataaggt aaaatcgtgt
     2101 gtttagttaa agcttattat aaaatcttga tatgcgatta atgatgtaat atgtgaataa
     2161 agttaagatt ataatagatt taacctgtgg ctaatagaat aaaattgtga ataaagttaa
     2221 gattataata ggtttaacct gtgattaata gagtaagagt gtaaatagag ataaacttca
     2281 atgtgggttt aacttgtggt taatagagta aggtgtaact aaagataaac ttcaatatgg
     2341 gtttaacttg tgattaatag agtaaggtgc aactaaagat aaacttcaat atgagcttaa
     2401 cctatgattg atagagtaag aatgtaaata gagataaact tcaatatggg cttaacttgt
     2461 ggttaataga gtaagagtgt aaatagagat aaacttcaat atgggtttaa cttgtggtta
     2521 atagagtaag aatgtaaata gagataaact tcaatatgag cttaacttgt gattaataga
     2581 gtaagagtgt aaatagagat aaacttcaat atgagtttaa cttgtggtta atagagtaag
     2641 agtgtaaata gagataaact tcaatatgag cttaacttgt gattaataga gtaagagtgt
     2701 aaatagagat aaacttcaat atgggcttaa cttgtgatta atagagtaag agtgtaacta
     2761 aagataaact tcaatatgag tttaacttgt ggctaataga ataagattgt gaataaagtt
     2821 atagttttag atgggtatta acctgttact aatagaatat gattaggtgt aaaattatag
     2881 agctgtataa gaaaatatta tagatatatt aaatattaga taataaatat ttatatagat
     2941 acacaaaaga aaaggcgata tgaagctaaa cttaatgact tcatatcgtt ttttatttga
     3001 aactcctttg tatttagagt gtgattttta atattgaggt tttatgtgat taagtcacag
     3061 ataaaaagta agtatcttga tataattctt atagattaat aatataggga ggttattatg
     3121 tctaataaaa aaaagaagta tgcaaatatt ttagcaaatg agtgcgttgc ttgtggtagc
     3181 tgtataaaag catgtccaag aagtgctatc tcagttccat gtggcattag tgcaaaaatt
     3241 aatagagatt tatgtgttgg atgtgggatt tgtgaaaaga tatgtccagc atctgtaatt
     3301 gagattataa caatattaaa agaggaagaa ggtaagtgtc atgaataata aaaaacattg
     3361 gtatgactat ttatggattt tttcttcaat atatttggta ttgggatttg taaatattat
     3421 tttcgcttgg attgggctaa tttgtttctt tgttccacta gctatttcta ttgttaaggg
     3481 aaataaagct tactgtaata agtattgtgg aagagggcaa ctgtttaatt tgcttggaaa
     3541 taaattgaag ctgtcaagaa atagagatat tcctaaattt attagaagta agtggtttag
     3601 atatgggttt ttgacatttt ttatggtaat gtttgccaat atgctttttt caacttattt
     3661 agtgtttgaa ggaagtagaa attttaaaga agtagtgact ttgttgtgga ctataaaaat
     3721 gccttggcaa tgggcgtatc atggaacttt agtttcacct tgggtggtac agtttgcatt
     3781 tggtttttat agtgttatgc ttacatcaac tgttcttggt ttgattacta tgatattgtt
     3841 taaacctcgt tcatggtgtg tttattgccc tatgggtact atgactcaga tgatatgtaa
     3901 ggcaaagagt aagtctgtat aggaagagat tttgtgaaga tttaaggggt ttaatgttta
     3961 aagctagagt tgatttaaga aaataagaaa gatataaata tctaaatatg aaatagattc
     4021 aagatgagta atagtttcta ttagctatga taggtatatg agtggagcat aaatgaagtt
     4081 tgtataaagt aaactcattt atgcttcttt tttacatgat acattactaa ttaaatagaa
     4141 aaacaatgta acaacttgta ttttaagtac gtcagagaaa tctgaagaaa tgataaatgt
     4201 tatagaagat tgtaaactta tgcttaaaac aagtaataga tatttaaagg aagcagatta
     4261 aatttcaaga agtcttacgt gcttgttgaa acataaactg gatttgttac agataaacta
     4321 atttatacaa tattttttaa ttaatatgtt ttcattacta tatattatat gttaatacat
     4381 aaaaaaatgg tggctgtttt attaaaggta ggtcaatgac atttcttata aaaataaatt
     4441 ctaactttaa attaacttag gagttctgta taataattat atgttgaaaa tattatgaag
     4501 ttttg
//
LOCUS       DAEPOZ010000257         4487 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.257, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000257 DAEPOZ010000000
VERSION     DAEPOZ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.257"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..653)
                     /locus_tag="KR440_003279"
     CDS             complement(<1..653)
                     /locus_tag="KR440_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0700285.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKE"
     gene            complement(622..1635)
                     /locus_tag="KR440_003280"
     CDS             complement(622..1635)
                     /locus_tag="KR440_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0700286.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKDNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(1657..2427)
                     /locus_tag="KR440_003281"
     CDS             complement(1657..2427)
                     /locus_tag="KR440_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0700287.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSMHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(2402..2692)
                     /locus_tag="KR440_003282"
     CDS             complement(2402..2692)
                     /locus_tag="KR440_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HBH0700288.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(2823..2922)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     regulatory      3542..3631
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            3684..3860
                     /locus_tag="KR440_003283"
     CDS             3684..3860
                     /locus_tag="KR440_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700289.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     gene            complement(3975..4331)
                     /locus_tag="KR440_003284"
     CDS             complement(3975..4331)
                     /locus_tag="KR440_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0700290.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKDVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
BASE COUNT         1502 a          748 c          514 g         1723 t
ORIGIN      
        1 aattctttct ttactattga tggttttttt gaaataacat aaattctgtt tgatagcatt
       61 atagcttcgt caatatcatg agttataaga agtattgttg aatttacttt tttcttaaca
      121 tctaaaagcc atttttgcat ggatgtttta gttaatgaat caagtgcccc aaaaggctca
      181 tctaaaagca ttatgtcatt agaatttata aaagttctca agaagttagc tctctgtctc
      241 attcctccag aaagttctga aggatattta tcttcatatc cgcttaatcc aaacacctca
      301 aaataaggtt ttaccattga cctagcttta ctcttttttt ctttttttaa aattagtggt
      361 agtgacacat tatctattat agttttatat ggcaagagta aatctttttg atacatataa
      421 ctatatttac catttatatt cactttgcca ctatcataat ctgtaagttt agttataata
      481 ttaaaaatag tactttttcc acatccactt ggccctaata tagaaacaaa ctcaccttca
      541 taaacatcaa tacttatatc ttttaaaact tcaatatctt tataagtttt tgatacattt
      601 tctatagata cttttacttt tttattgtgg taaaaattca tttgtgtaag catcacttgc
      661 cttcatatcc ttatctatta atttatattc ttttaagaat gatgtatagt tgttccatac
      721 actatctttc atttctcccc atcgtggtgc atcatctttg tacttgcttg ctaaatattc
      781 ttgactcttt aaagctagtt tttcatctac ttcaggtgca tgttttacta aaatttttgc
      841 tgattcttct ggatttttta ttgcatattc ataacctttt gttgtagctt ttaagaattt
      901 tttagcaagc tctggattat ctttaagcac agtttcatta gaaattataa gtggtgtata
      961 ataatccaat ctctcatcta aatcttttac tggtataaag tttaagtcat atccaattaa
     1021 atcagcttta actgcatccc aaccttcaaa tatccatgca aaatctacag ttttcatagc
     1081 tgcaaagaaa tcatcttgac cagtattaac tatctttaac ttattaaaat cagctccatt
     1141 tttcttcata actgctttaa aaacagcttc ttctgatgga gagccccaac caccataagt
     1201 ttttccttca aaatctttag ctgttgttat attcttttct tttggtgatg caaacccaga
     1261 agtattatgt tgtataacag ttgctacagc ttttattgga agtggatctt cactagtctt
     1321 tgcataagta acttgttctt gatagcttat accaaagtca gcttttcctg ttgctactaa
     1381 agtttctgct ccactttcag gtggttgtac tatttcaaca tccaaacctt cttctttata
     1441 gtaaccctta tcaagtgcta caaataatcc agtatgattt gtatttggtg tccaatcaag
     1501 caccatagtt acttttttag aagaagtatc tttatcattg tttttgtctt tattgttatt
     1561 tgaagaacat ccagttattg ttgccaatgt aaaaacactt actaaaccta atattcctaa
     1621 ctttttaaaa ttcataattg cctccctcaa atacctttaa tcaattaatc tatgtcttat
     1681 aacaactttt tttacagcct gtacaattcc tacaaacatt aaactaaata taactactaa
     1741 tatagtagat gcaaatactt tatcaagtgc atatgcactt tttgacctaa gcatataaac
     1801 accaagtcct ttcgttccac ctagccattc tgcaacagtt gctgccatta cagcatacgt
     1861 tgcagatatt ttaagtccag aaaaaaattt atccattgcc ataggaaatt taaggtgaat
     1921 gaatgtatta actttatttg agtccatagt cttaaataaa tttaaataat ctttatcaat
     1981 attctcaatg ccatctacaa aacttattaa tataggaaaa aaacaagtaa gcattaccat
     2041 caatactttt ggcattgttc caaatccaaa ccatatcata aaaagtggtg ctataattat
     2101 tgtaggtatc atctgtgtaa ccaacataat aggataaaga cattttttta ttattgatac
     2161 aaaatccatc aatattccaa ttaccaatga tattataatt gcgactataa aaccaagtat
     2221 tgcttctcct atagtaacca cagcatgcat ggataaattt tgataatctt taaaaaacac
     2281 attaattata tctactggtg tgggtagtat atactgagga acttctaaaa ctcctaccat
     2341 agattgccaa agaataagta taaacaaaaa tgttgctatt ggatatatac tatctttgac
     2401 tttatctatg atttctaagt ttttcatcta tagtcagacc tccagctttt tcatctactc
     2461 tagctactgt tatgactctt tcacatccaa gattaaaagg gattttatgt acctttttta
     2521 ataattcaaa aacttcatca aaatcacctt caacagtagt gctcattgct cctatttcac
     2581 atttaagacc acttttttca atttcagcaa tacaagcatc tactacttta tacatttctt
     2641 cttcaccttt gtaaggtctt agtggcatta ctgctatgtc tgcgattacc atttaaatca
     2701 ctcctttaaa aataattaat acctactagg tatattttgt tttttattaa aaaattttat
     2761 tactaaaaag atattatatt taaataaaag aaaagtctta tccaaaatag gataagactt
     2821 atttttatct taactggtct ccctacactg gcattatcca aacaggttca aagggtcgag
     2881 ataaaatctc ctctcagcac attagctccc cgaaatatat tatatctttt ctacttgatt
     2941 atactacact tatcatattt taacaaattt tttatttata ttagatattt tatccgttca
     3001 aaaatatcta atatacattg tacattatta caccaaataa attatcttaa aatggtaatt
     3061 tatcaacatc ttccaaaact agattaactg aattttctag tttttatttt gctatttcaa
     3121 gcatatttgg atgtatatca attccataca aatattgttc taaatttctg ttttcactaa
     3181 tctaatttgc aatcgttaca gattacagat tagaaatagc ttttagactc tacttatctt
     3241 tatctacaaa aaatccttca ataaattcag taataatatt cagtaatcta aaaataaacc
     3301 tctatctgga tttttaagat acaagccttt taggaagtat cttttgtgat aatataaaaa
     3361 ttactcgtca ttatcatcaa aatcctcagc aaccaacatt ttagataaga atcaaactct
     3421 ataatataat tattttatcc aatttatttt tgagaaataa aatatttttt taatttttaa
     3481 attttttaat atgcaccttg aaaaaagaaa cgttttccta tattatggta atagtagaat
     3541 aaaactaaat aggcaaaact agagaaattt agtgacgcaa agctataggg actaagactt
     3601 atgaaatgag ttatgtcagc cagttgccaa agagatattt tctttttgtt tttatgaatg
     3661 ttttttaggg ggatatatga tatatgttta tggataaaat ggatagatgt acacatatac
     3721 taactgcata tatatgcagt tcacgcgatt actgcaattt tatagataca caattaaatg
     3781 attttatact agaatatgga gagaatgtgg tagaatcttg tttgcatcaa gtgatggtat
     3841 tggtaagcag atataattaa agaaatttta ttttctagaa aacccttttt agttttatct
     3901 acgttttata attcattaaa gaactaatca aaataagctt cattatatat ttttcatgaa
     3961 gtaaacagtt gagtttatct catatactgt ttaccccaat tacataattc taataatata
     4021 gattccaatg atttaccacg tttagtagac aaatattcaa cttttggagg aatagtcgga
     4081 taagaaattc tcttaataag tccatctctt tcaagatact ttaattcttg tgacagtatc
     4141 ttatgactta tatgaacaac atcttttttt aattgactgt aacgtattgg ctcttgtttt
     4201 aaaatatgcc ataaaataat tgccctccat ttacttccta tcacagtcat ggtaaaacca
     4261 attgaacaat taaaatcttt ttgtctttct tcaatatttg gaaattcttc aaaattatta
     4321 tctatatcca tctcaatcct ctctttcaaa atagtaagta tctaactaaa aagtgcattc
     4381 ttgtttagcg tactaattcg taatataatc ttgattaaaa aatagctacg cacactcgtg
     4441 actttagtca tgagttagta gctaaaatag tcaacatata gggaaac
//
LOCUS       DAEPOZ010000258         4480 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.258, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000258 DAEPOZ010000000
VERSION     DAEPOZ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.258"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            117..617
                     /locus_tag="KR440_003285"
     CDS             117..617
                     /locus_tag="KR440_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBH0700291.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIISGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            674..1660
                     /locus_tag="KR440_003286"
     CDS             674..1660
                     /locus_tag="KR440_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBH0700292.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            1710..2432
                     /locus_tag="KR440_003287"
     CDS             1710..2432
                     /locus_tag="KR440_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HBH0700293.1"
                     /translation="MENFNKNAKIIGIGVEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            2461..4395
                     /locus_tag="KR440_003288"
     CDS             2461..4395
                     /locus_tag="KR440_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0700294.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFIEESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALDGGYFFPVRWIEYGKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNKYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
BASE COUNT         1849 a          338 c          803 g         1490 t
ORIGIN      
        1 tagttcgcta aaataatttg tttttataaa attactgttt aagtttatag attttgtata
       61 aaataaagtt atgttattaa tttttttaga aataaaactt ttactaggag acagatatga
      121 gaaaaaatag tattaatata gcactaactg cagcaattac agcaatctat gcagttttaa
      181 cattatcatt gggatttata agttatggac caatacaatt tagggtagca gagattatga
      241 tgcttttggc atttttagat aaaggatata tagtaggtct tacattaggt tgttttttag
      301 caaatgtaat aggaccatat ggtgtacctg atataatttc tggaacatta gccacattta
      361 taagtgcaag tatgatttat attactagaa agttaggtaa acaaaataaa agtactttag
      421 ttatagcttc tttatggcca actgttataa attcgattgt ggttggtcta atgttaaatg
      481 tattctttgg gcttccatta attctatcta tgatacaggt aggttttgga gaatttgtag
      541 tagtaacaat tgtaggagta ccatttttct gctttttaat gaaaaaatat aaaaatgtca
      601 ttaatattaa attttaaata gtgtatagta atttaattaa ttgagagtat tattagtgaa
      661 aagaggtgtt tatttgtcga aggtaaaaag attgactgat gtttggatat atattaatga
      721 taagcaaaag tttacaataa aagagttgtc agaggaattt aatctttcag ctaaaacaat
      781 acaacgatat ttaaaagaat taaataaaat gggacttcca attcaagcag aacaaggtag
      841 aaatggaggt tatagggtgc taaataatag ctatatacct cctgttatat ttacagaaaa
      901 agaagttatg attatactat ttgcacttaa gtcacttcag atttataatt ataagttaat
      961 ggaaatagaa attaattcta taatgagaaa agtaagttat gaacgtaaaa gcagtataaa
     1021 agtaggtata gaaaatatta aaagatacat tggctttgta gctaatgata ctaatgatgg
     1081 agattttaag agtagtaatg taactgagtt ttttagagct tcttcagaat ctctaatttt
     1141 aaatattaag tataagtgtg gtaatcagat tttggaaaaa aacgtatgcc ctataggtat
     1201 atatttaaat aagggagcat ggttttcacc agcatatgat tgtaaaagtg ataaaataat
     1261 attagttcat atagataata taattgatat aaaaagaaca ggagaaagta aaaaaataca
     1321 tataaacttg gaagaatggt tacatgaagt atataacaat ttatatggaa atagttcaac
     1381 tactaaaaaa tttggtgaaa ttatttatct aaatgtatta ttaactaaag aaggtgtttc
     1441 taaaataaag gacagttctt ggaatttaga tatggagcta aatgaagatg gtagtggaat
     1501 aattagtaca ttcataaagg ttgaagatat agattgttat gcttcgattt tattttttat
     1561 tggaaataat gctaaggtta tagagccaaa atttttgaat aagctacttt atgataaggc
     1621 caatgagtta agatattttt atgaagaaaa tatggtataa tatttagaaa tgttgaaatt
     1681 aaataattat taaggagata tacgttataa tggaaaattt taataagaat gctaaaataa
     1741 ttggtattgg agttgaaggt attaatataa taaatgaagt agaagaaaaa ataaaagcaa
     1801 atatggatat agaaaaaata aacataaatc aagagattga aaaagaatat gttagaagtc
     1861 ttttagatgg tgtagatata ttatttttga tatattcatc tgaagataaa catattagag
     1921 atattataaa agctgtttca tatatgtcaa acgaaagaag agttctatct ataggaatgg
     1981 attgttcaga gaaagaaaat aaagaagatt tagagcttgg gagagagttt aaaatcaata
     2041 atgatagtat atttaaattt gttgatttaa tgaatatcat ggtagagtct atttctgatt
     2101 cttgcatgat aaatatagat attactgatt taaaagaagc tattgtagga gataaaggaa
     2161 ttaagtattc ttttgaagag tttgaagata caaagtcata tagcgagata gctgacattt
     2221 tatttgacag gatggaatat attggtgagg aatttataag taagaaagga attgtatttg
     2281 ttgaaggaag tccagaattt tctataatgg aattgaatga tttaataagt aatattcaga
     2341 gcaaggtaga agaaagctat gaagtaatat tttctttata tataaaggaa aatttaaatg
     2401 gaaatattag agtaggctta ttgtataatt agtatatttt agatattaag gagagtgtat
     2461 atgtctgaaa atataagagg tacagagtat tggttaatgg aagaattttt ttatcaggaa
     2521 acagacacac cacagatttt tatagaagaa agcataattg cacttggatt tgatataaaa
     2581 ataaatttaa aaaaagtatt cagtatgggg aaaaaatcag aaaagtatat taatgagaat
     2641 tgtaatgata aagattttat aaaaaagttt atgtcgatta aaaaaggaga ttatttttta
     2701 tataaagcat atcaaaatat agaagaaaaa gttaaatgta atgtgtctat tggcatagtt
     2761 gaagaagatt ttgaaaatgg atatgaatta agtcatattt tagggcatac actaccagta
     2821 aaatggatgc ataattttga tacagtttca aattgtaaag tatatacaag tgaattttat
     2881 aaattaaata ataatgatat tagttcatat ttagaaatta ttaagtcaca agataagaaa
     2941 ttattgagaa aaagaataga agaaagtgaa aataataaat ggtatttaaa aaactattat
     3001 tataaaaatg tagataagtg tgtagatgat gaagaattat ttattcagcc cattaatcta
     3061 gaacaattag aaagcctaaa aaatataaag acagatgatt atattttatt gtataatagc
     3121 gagaaagtaa tttgtgaaaa tgaccataca atgaagagtc tatttggcgt atgtcaagtt
     3181 atggaagggt tcgataaaga gaaagtaagt gctttggatg gtggatattt ttttcctgtt
     3241 agatggatag agtatggaaa ggagaatata gacggaaatg catgggttat atttgaaaat
     3301 aatagtatag ttgaaaagta tgtaaaaaga tttactaata aaatgccatt aaatactata
     3361 ctttatggac caccaggaac tggtaaaaca tacaatacaa aacagatggt ttacaatcta
     3421 gtaagtgaaa ccagtctaag aaacaaatac tatacaggtt acttagatta taaaaacaat
     3481 gtagaatttt gtacatttca tcaatcatat ggatatgagg aatttataga gggtcttaag
     3541 tctgatggtg aaggcaattt taagccagaa gatgggatat taaaggatat atctatagaa
     3601 gcatgttata atgcattgaa atttgaaaaa aagatgagct ttgagttgga aaaagatagc
     3661 ttgactttag aaactataaa agacaggaaa aagaaattag tattagaaaa tatagaatat
     3721 agtgagagtt ttaattttga agaatcagaa aactatgttt taataataga tgaaattaat
     3781 agagggaatg tatctaaaat atttggtgag ctaataacat tattggaaga tgataaaaga
     3841 cttactaaat caaatcaaac tattgtcaaa ctaccatatt caaaggaaaa attttctcta
     3901 cctccaaatt tgtatatagt tggaactatg aacacatcag ataaatcaat agcactttta
     3961 gatatagctt taagaagaag gtttaatttt gaagaaatta tgccagatta tgatttattg
     4021 tctattgtag atggtataga tattagaaaa atgctatata caatcaataa aaggatagag
     4081 tttttatatg atagagacca tgttattgga caagcttatc tacttgctgt agaaaatatt
     4141 aatgatatag tagtagtatt tagaagtaaa ataataccac ttcttcaaga gtatttttat
     4201 tatgattcag aaaaagtagg tttgatttta ggaggaattg gaagaaatga aaaagataaa
     4261 tacattgttt ataaagaaaa acttatagct tctgagttat ttaaaaataa taatgcctca
     4321 ataatggaga gtaaagttaa ctattgtata aaacaaaaca tatctagtga ggagctaaaa
     4381 aatatatatg agtaaaaata tatatgttaa ggaaacctat gaatggataa gggtaggtaa
     4441 tggtgaaaat gagcttacag aaattgaata tgaaaaattg
//
LOCUS       DAEPOZ010000259         4469 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.259, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000259 DAEPOZ010000000
VERSION     DAEPOZ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4469
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.259"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            212..589
                     /locus_tag="KR440_003289"
     CDS             212..589
                     /locus_tag="KR440_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH0700295.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
     gene            582..1616
                     /locus_tag="KR440_003290"
     CDS             582..1616
                     /locus_tag="KR440_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBH0700296.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENAVGNNITQIDISTDNNLMLYVAYLWLVVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDVNCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLTDCSLKDKKEYALALIKSIKLSKNYNSGILTTEFNKTSLE
                     KRLDSIVKKKGKNGILVLLILITLFSVTYFNFESISFDNSNSLRNMGILQKSGVDLKE
                     EDDISMRVIN"
     gene            1682..2269
                     /locus_tag="KR440_003291"
     CDS             1682..2269
                     /locus_tag="KR440_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBH0700297.1"
                     /translation="MKNLKKILIITGCLTLISTSLVFANTTTDVDTSKDDDSIISSCT
                     ITGYEFTYENAPQSVKFDYEKKCEALNIKPSKNDIIFVNKDSIDANDVENYVEPKAYI
                     IRYKKGYVFEVTGPKSYTLNVSNTVVGYNRVESGNPVHLVQLLCRTLDGSSIDDDSEF
                     GPATYNAVKSLQGRLGLTRDGIVGRSTWDAAGRRL"
     gene            complement(2647..3408)
                     /locus_tag="KR440_003292"
                     /pseudo
     CDS             complement(2647..3408)
                     /locus_tag="KR440_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612258.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4016..4393
                     /locus_tag="KR440_003293"
     CDS             4016..4393
                     /locus_tag="KR440_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH0700298.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDATVTSKDVIEVMEQKYGWKQTTT
                     LTLLSRLVKRGFLNAQKIDRYTHYTVIVKHKDYLSFETKDFLSNIHDDSLKSLISALH
                     DDENIGKDTLDFLESYFDNLKNE"
BASE COUNT         1710 a          469 c          635 g         1655 t
ORIGIN      
        1 aatgtgtaaa taacattcta cactattgta attaaatatg taaaaagtaa aatatttaaa
       61 tagatatatt ttaaaatcta acttttaata ttgtaaaaaa attagaaatt taacaaaaag
      121 tacaaaaatt cacatttttt tcataaagtg tttgactaaa aaattcacat gttgtagaat
      181 taaggtagca ggagttttga gggaggtgta aataatgata agaaaaatac cacaagcaga
      241 actaaaagta atgaaattca tttggaaagt agatgttaca gtgacatcaa aagatgttat
      301 tgaagctatg gaacaaaaat atggctggaa acaaactact acactgacac ttttatctag
      361 gctggtaaaa agaggattct tagatgctca aaaaatagac agatatacac attacacaat
      421 aagagtaaaa cacaaagatt atttaagttt tgagacaaag gatttcctaa gtaatataca
      481 tgatgactct ttgagaagct taatttcagc attacatgat gatgaaaata taggtaaaga
      541 taagctagat tttctagaaa gttattttga taacttaaag gatgaataat tatatgctat
      601 atgtttttga aaatcttata aagacaacta ttgtatgtag tcttggtatt tgtctattat
      661 tatttttgaa aagatattta tttaagaagt ttagtaagag attcaattat tatatttggt
      721 tgataatagt ctttagaatg ttactttttc tatttaatta tacaatagtg tatgaagtaa
      781 aagaacccaa agaaaatgca gtaggaaata atattacaca aattgatatt tctacagata
      841 ataatcttat gttgtatgta gcttatttat ggttagttgt aactatagtc attgcagttt
      901 atacatttat aaagtataca agatttaaaa atctagttgt tgatgtatct tatgatattg
      961 aagataatga tgtaaactgt atctacaaaa acttgttgaa agaattaaat ataaaaaaga
     1021 aaattgaatt aagaggttct gatgaattga taagcccagc aggcatggga ttatttaaat
     1081 cttacatatt cttaccagac tatccttata gtaaagatga actaacttgg attttgaaac
     1141 atgaattaat gcattttaaa aataaagata ttttaataaa gtttttagtg ttatctgtta
     1201 gaataatata ttggtttaat ccacttgttt atgtaatgag caataaagta aatctagatt
     1261 gtgaattatg ttgtgatgaa agtgttttga ctgattgttc tttaaaggat aagaaggagt
     1321 atgcattagc acttataaag tcaattaaac ttagtaagaa ttataatagt ggtattttaa
     1381 caacagagtt taataagaca agtctggaaa agagacttga cagtattgtt aagaaaaaag
     1441 gtaaaaatgg aatattggtg ttattgattt tgattacatt attttcagtt acatatttta
     1501 attttgaaag tattagtttt gataattcaa attctttaag gaatatgggg attttacaaa
     1561 aatcaggagt agatcttaag gaagaagatg atattagcat gagagttata aattaataga
     1621 ttaatgtatg taatgaaaag gtatattgtc gtattattaa taaattagga ggaattatga
     1681 aatgaaaaat ttaaaaaaaa ttttaataat tacaggttgt ctaactctaa tatctactag
     1741 tttggtattt gcaaatacta ctactgatgt agatacatca aaggatgatg attcaataat
     1801 aagttcatgt actataactg gttatgaatt tacatatgaa aacgcaccac aatctgtaaa
     1861 atttgattat gaaaaaaaat gtgaagcgct aaatataaaa ccatctaaaa atgatataat
     1921 ttttgttaac aaagattcta tagatgcaaa tgatgtagaa aactatgttg agccaaaagc
     1981 atatattatt agatataaaa aaggttatgt ttttgaagtt actggaccta agagttatac
     2041 attaaatgta agtaatactg tagtcggata taatcgtgtt gaatcaggta atcctgtgca
     2101 tttagttcaa cttttatgta gaaccttaga tggttcaagt attgatgatg atagtgaatt
     2161 tggacctgct acatataatg cagttaagtc acttcaagga agacttggat taacaagaga
     2221 tggaattgtt ggaagaagta cttgggatgc agctggaaga cgtctttaaa aagaaagcta
     2281 ttgcacagat aattttaaat tgctgaatct gtaaacattt acatgtattc tttaaataaa
     2341 aatttatttt ttaagtgata attaaatttt tactaatagg gctatggctt taaaaatcat
     2401 agccttattt tgatatcttt tccatttttg caaaagctat taacccttta agaaaatttc
     2461 ttgttttttt gaaaaatttc tttcttcaaa ataaatactt aaatgagcta aaatttgagc
     2521 acaatttcta attttctgag tccattttta agaaatatat atggttgtta agtatagtga
     2581 tttgtgcaaa taattatcca aagtaaaaat agatttcaat ttagtaactt ttcttaataa
     2641 tctattctaa cttcccattg aatttgtagt atatatcgat gtatttattt cttgagagta
     2701 cttaaaatat gttactaatt cattccaatt gtttcaccaa cctcttaata ctattgtata
     2761 ttgattgtac tattttttct ctagtgatag ctcacttaat gctacctctt ctgtaattgt
     2821 agcatatctt tctttaaatc ttttacagat tcttttctat ttttatatga tacataattt
     2881 aaagtatttt taatttgatg cacaacatat ctttgtactt aagtatcagg aaatatcgct
     2941 ttaattgcat tagaaaattc aactagtcta tctatagaag ctgctattaa tacatcttta
     3001 attcccatgt ttctagcatc tataagcact tttagccaat actttgaaat ttcattttct
     3061 ccaatccata ttcttaaaac ctcttttatg ctacatatag ctatatagac tgttttgttt
     3121 attattcgac ctcacttcta actttataat gtatagcatc tataaataca attgaataaa
     3181 ttatattaag aggtctaagt taccatttat gagttaaagg aataacttta tcagtagttt
     3241 tagttattag tgaatgagat aatatcaatt ccataagtac ttttaagatg acttaatata
     3301 ttttgagtag acatgccttt tgagtatatt ccaatcactt gattttttat tcctaatata
     3361 tcattttcta tcataaccta aaagttcatt aagctccgct tctaacattt aatggattgt
     3421 actttcaaat aaatctttta gtatatcttg aatatctgta attgttttgg catcatattc
     3481 ttcaataaat ttttttaata ttttagtttt tgttaaacat aaaaagtgaa catcttcctt
     3541 tgtatagtag tgatatttta attatcacaa ctttttagaa gaatacaaac tttattttaa
     3601 agacactgaa aatggtgttt ttataccttt atttgctatg tataatttta cttataacat
     3661 aaacttatat aaaacagata ttttaataac acaaaggtac aattaggaga ttgttaaaat
     3721 ttattagata ttactctagt ttatttaaaa aaaagtatta taatattcct tgtgtgaaaa
     3781 tataggaatt tttattaaat aattttaatg tggaaataac attctgcact attataatta
     3841 aatatgtaaa aagcaaaata cttgaacaac tatattttaa aatctaactt ttagtatttt
     3901 aaaaaattag aaaattaata aaaagtacaa aaatccacat tttttcataa agcgtttgac
     3961 taaaaaattc acatattgta gacttaaagt agcaagagtt ttgagggagg tgtaaatgat
     4021 gataagaaaa ataccacaag cagaactaaa agtaatgaaa tttatttgga aagtagatgc
     4081 tacagtgaca tcaaaagatg ttattgaagt tatggaacaa aaatatggtt ggaaacaaac
     4141 cactacacta acacttttat cgaggttagt aaaaagagga ttcttaaatg ctcaaaaaat
     4201 agacagatat acacattaca cagtaatagt aaaacacaaa gattatttaa gttttgagac
     4261 aaaggatttc ctaagtaata tacatgatga ttctttgaaa agcttaattt cagcattaca
     4321 tgatgatgaa aatataggta aagacacgct agatttttta gaaagttatt ttgataattt
     4381 aaagaatgaa taattatatg ctatatgttt ttgacaatct tataaagaca actattgtat
     4441 gtagttttgg tatttgtcta ttattattt
//
LOCUS       DAEPOZ010000260         4466 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.260, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000260 DAEPOZ010000000
VERSION     DAEPOZ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.260"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(48..593)
                     /locus_tag="KR440_003294"
     CDS             complement(48..593)
                     /locus_tag="KR440_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBH0700299.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(711..2534)
                     /locus_tag="KR440_003295"
     CDS             complement(711..2534)
                     /locus_tag="KR440_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0700300.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVKEMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRLLNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGVLASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KAGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(2547..4321)
                     /locus_tag="KR440_003296"
                     /pseudo
     CDS             complement(2547..4321)
                     /locus_tag="KR440_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT         1447 a          796 c          392 g         1831 t
ORIGIN      
        1 aatcataaat gatatactcc taaaaatcta cttaacttat attaaattta ttttccaaaa
       61 tatctattta ataatcctaa gaatgcttta ccatgtctag cttcatcttt acacatttca
      121 tgtactgtat cgtgtatagc atctagtcct aattctttag cttttttagc taattttaat
      181 tttccatcag ttgcaccata ctcagcatca actctaactc ttaagttttc ttttgtatct
      241 gcaactacaa cttctccaag aagttctgca aatttagcag catgttcagc ttcttcaaga
      301 gctattctct tataagcttc tcctacttct ggataacctt ctctatcagc ttgtctactc
      361 attgctaaat acattcctac ttctgtacac tcaccaacaa agttagctct taatccttcg
      421 attatttctt catctacacc ttgagctact cctattctgt gctcatcagc ccaatttttt
      481 tcgcctttca tttcttcaaa tttatcagct ccaactttac atactggaca ttgtgctggt
      541 gcagcatctc cttcatgtat atatccacat actgtacaaa caaatttctt cataattact
      601 ttcctcctaa attttatata tcaaattaaa ttttttattt attaagttag tctatatata
      661 cccgcaccat atacctttaa acaatttttt taaaagtttt ttctaaatac ttattttccc
      721 tgtgtcaata atatgtttct ataataatgt ccctttaatt ttatcaattc tttatgattt
      781 cctgattcca caattctacc attttctaaa actattatat tatcacaatt tgatatggta
      841 cttaatctat gagctataat tatacttgtc tttccataag ttaaattatt tattgcttct
      901 tgaattttag cttctgttgc tatatctatg ttacttgtag cctcatccaa aataagtatt
      961 ggagcatttt ttaatactgc tctagcaata cttattaatt gtttttgacc ttcacttaaa
     1021 tcttcacccc tatccgataa tacagtacta tatccttcac gcattttaac tataaattta
     1081 tgtgcatttg ctattttact agcatatata acttcttcat cagtagcctc cagatttcca
     1141 taccttatat tttccataac tgtatcattg aatatataac cttcttgtgg aactactcca
     1201 aaacaagttc tcaaatagtt tctgttaata gagtatatat ccttaccatc taaaaatata
     1261 cttccttcat ctatatgata aaaatttgta attagattta taatcgttgt ttttccactt
     1321 cctgtctctc ctatgatggc atttgatgtg ccagctttga tttttaggtt tatattatct
     1381 aaaattttct tttttgaatc ataagaaaaa cttacatctt taagctcaat ttctcccttt
     1441 acaaaatgag agtttaaatt accatcttta ttatcgttaa tctcactttt aaacatgtta
     1501 tgcacttctt gctcttcttg ttcatctata atttcaaaaa ctctttcaca tccagctatg
     1561 gctgaaagta atgtattaaa tagatttgct aattcattta aaggtcttgt gaattgtctt
     1621 acataactta aaaaactagc tataacccct atggttataa tatctttaga tgcaagaaca
     1681 cctcctataa tacatatagt tgcaaatcct atattactaa tcacattcat aataggcatt
     1741 aaaagagaag tgtaaatttg cgatttaagg gaaatattta gaagtctatc attgacttct
     1801 ttaaaatcat ttacagattt tctttcataa ttaaaagatt ttactacatt tatgtttgat
     1861 atcatttctt caatatgtgc atttagtttt cccaactcaa cttgttgttg cttaaaaaaa
     1921 actctagttc tctttgcaat aaaattactc acaaacatta ctatcggtat taatattaca
     1981 ctgcaaaatg ttaaaattgg gcttaataca aacatcataa taaaagtcat caatatgttt
     2041 aagattcctg taattaactg tactaatgaa tttgatattc ctgtactgat attatcaaca
     2101 tcatttgtca ctctactcat aatatctcca gtagaacttt tatcaaaaaa ggttattggt
     2161 agatttttaa attttaaaaa taaattactt ctcatttctt taactatact ttgagataat
     2221 ctagctgtac atatcccctt agacagttta actgaaaact caattatata aaatgaaagt
     2281 aaaataatta ttccttttaa gaaaatggat tttgaataag tatcgatatc tattatgtca
     2341 actacctttc caatagtata tggtattaca gttgatataa ttgaatcaat tattactagc
     2401 aaaaaaataa atctagtaat cttcttatct tttttaaagt acataaaaat tcttttaaac
     2461 gtttttttaa aatctttagg cttttctttt ttctttaatc tactgacctt ttgatttcca
     2521 gtacttaagt tcaatatact tcctccttaa tttacatctt cacttactaa attctgcaac
     2581 gcaaaaattt ttttataaat tggagaacta accagtaaat cttcatgtgt agcaaatcca
     2641 accacctcac cctcatccaa gactaaaatc ttatcacagt caattataga actaatcttt
     2701 tgtgaaatag ttattacagt acatttctta ctttcctttt ctcttaaatt acttatatac
     2761 tcttttatat tctttcttat aattgtctct gtattaatat ccattgcact aaaacaatca
     2821 tcaagaatta atatttgtgg ttttttggct atagctctag ctatagtaat tctttgtctc
     2881 tgtcctcctg ataaattttt acccctttga aataaaacct catcataaga attttcgagt
     2941 ttagttataa attgatttgc atttgctatc tcacatgatt ttataacatc ttctctactt
     3001 ataccctcat ttcccataac tatattttct agtatacttt ttccaaacac actgctattt
     3061 tgaaatacta cacctatttt ttcccttaaa ctttcttcag aaatactgta tatactacgt
     3121 cctccaatct taatatcacc ttatcaatta tatataattt ttgaattaaa tttactaaag
     3181 aagtctttcc tgaaccagtt tgaccaatca caccaatatt ttgaccttct tctattttaa
     3241 aggatacgtt ttttaatatt ttttcatctg tatttttgtt atacgaaaaa tctacatttt
     3301 caaaaattat atcacaattt acaaacttta aatctttttc tccaacatct aaactttctt
     3361 ctctctcaat tacttcttca ttctcatcaa ccaaaacctc acttattctc tcataagatg
     3421 cttttgccct aacaaaaaca ttaaaaacat gtgaaatcat tataagagat gttaatattc
     3481 tagtcatata gtttacataa gcaactataa tcccaatttt aatttctcct gaataaatta
     3541 aatgccttcc aatcagtaat attattatta tggaaacatt aatgattatt gtaattgtcg
     3601 gtctaaaaaa tgacatcata gttgtagctt ttctactatt tttaaaaagt tcatcattta
     3661 actcttcaaa gctttggttc tcataatcgt atctacaaaa tagtttaact acttttattc
     3721 cacttaaaaa ctgtcttaat tttgaattta ctttatctgt actttcttgt acatttttaa
     3781 ataatctata ccctatcttc atattaagca atattataat aaatataatt aaaactgcaa
     3841 ttagaataat tattgacatc tttaaattta attttatagc tataattaaa cttcctatac
     3901 acattattgg tgactttata aaaactctca tcattccatt aacgaattgt tgcatttgat
     3961 tgatatcatt agtaattctt gttattaacg aagctctacc aaatttatct atttgtttta
     4021 atgagtatgt atttatcttt tcaaatacat cctgtcttaa atcataagaa aaatgctgag
     4081 aaactcttgc cgagattaca cttcttatta ttgcaaatat tgcacctaaa aaagttatac
     4141 ctatcattat agctcctgtt gatagtatgt agtctaggtt cttatttgaa acccctatat
     4201 ctattatttt tgccattata gttggttgag ctaaatcaca aaaagcttcc aatgttaaaa
     4261 aaaatactgc tgttagaaat aatactatat atttttttat gtatttttta aaatacttca
     4321 ttcaatcatc ccttcataga attttctcat taccaaaagt atatcataat ggctaattta
     4381 attgattata tcaattataa atatttgatt tattattaat ttttatacca tatattttat
     4441 aaattatttg tttttatttc ctatat
//
LOCUS       DAEPOZ010000261         4441 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.261, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000261 DAEPOZ010000000
VERSION     DAEPOZ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.261"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(200..1219)
                     /locus_tag="KR440_003297"
     CDS             complement(200..1219)
                     /locus_tag="KR440_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBH0700301.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLHGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQAITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            complement(1395..1934)
                     /locus_tag="KR440_003298"
     CDS             complement(1395..1934)
                     /locus_tag="KR440_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HBH0700302.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPILVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(2035..2715)
                     /locus_tag="KR440_003299"
     CDS             complement(2035..2715)
                     /locus_tag="KR440_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBH0700303.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PQNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            complement(2744..4033)
                     /locus_tag="KR440_003300"
     CDS             complement(2744..4033)
                     /locus_tag="KR440_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBH0700304.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
BASE COUNT         1513 a          770 c          397 g         1761 t
ORIGIN      
        1 aatcgtcaaa actttttttg atactgttac aaaattgtaa aaaaaaagaa aaaaacacca
       61 aaaataaatt tctttttagc actttttcat acttataatt tgacatttta acacttggct
      121 aagtatacac cttaccttaa cttaaaatta aatttattta agaatatata ttataaattc
      181 ttatattttc taatataagc tatatactat cagctggata aacaatacta ctttgtcttc
      241 ttatctcatc cataatttcc attactatta ttgaattttc atgagaattt atatttgatt
      301 ctattttatt gttatttata agacttataa actctacaag ttcataatac attccttcta
      361 tttctctagg cttttcctct gctttttctt ttggaataga tatatcttct tctcttccat
      421 ctctgtattt aataatagct ttttcaaata gattaagctt ttcaattatt atacttccct
      481 cttctccttg tatttcagat ggtatataag aatcagctac tttagaatac tgaattattg
      541 catccatatc atcatattga aatacagctg aaccttctcc atccactcca gtttgaagta
      601 tatgagaaac tgcctttaca gtttttggag caccaaataa attcaccata ggatgtatac
      661 aatacactcc tatatccatt aaagctccat ttgacaactc ttttttaaag gcattttcta
      721 taattccatt tttatattta tcatatcttg aagaatactg acaatagctt ccaaaaaatc
      781 ttcttatttt ccctatttta tacaagtttt ctttaactgc tttaaagttt ggaacacatg
      841 taattctcat agcttccata agcaatacat tattatcttt ggctgttttt atcattgatt
      901 tgacttcttt taaattagat gctaacgact tttcacaaag cacatgtttt ttgtgtttta
      961 aacaagtaat ggcttgcttt gaatgtaatg catttggaga agctatatat acagcatcta
     1021 tatccttcga tttagccatt tgttctaaat catcaaaaaa aatacttgct ccatgtagag
     1081 ttccaaattc tctagctttt tctaaatttc tagaatatac agcaaccaat tcaaaatctt
     1141 taaccctaga tgcagctctt agaaatatat ttgctatatt gcttgtccca acaataccaa
     1201 atcttatttt acccatcata aatcactccc cattttctac actttatttt aattttaata
     1261 aaaaacttta caacttatag atatattata aaataatttt atcaaataaa aaagaaagct
     1321 aattttaaat gatttcacca ttttaacata gctttctttt aactacaatc taaattcaac
     1381 ttatattttt aaagttatct ttctactata tctttatttc ccctcaaaaa ttttatagta
     1441 agaatttcat ccacaaacaa tgttactaaa agtatagtta caagaattgg ccaataaggt
     1501 attataagac tatataaact atcaactata tacacttgaa aatatctaac tccaataaat
     1561 gtcaatatag tccaatatac agaaaacttt agacatacta ttcctctact attgtactta
     1621 taatctgaat aatcccaata gaccttatta aaatatactt tcataagtat tcctgtaata
     1681 tattctacta ctgtcggcac tactagacat aataaaagca atgcaaatat attattgggg
     1741 tcaataaatc ttgagacctc tattataatc gacattgcaa ttgcatacat tggtttaaat
     1801 ggaccattta aaaatccatc ttcttgaaaa tgcccttgtt tataataact atataagttt
     1861 tcaattatcc aacctataaa accatacaag aaaaaattaa ataaaaaata taatagagaa
     1921 cctacattat ccatgaatat tcctcctagc tgtttatact tattttttct catattcatg
     1981 gatttattct aaatcaaaga aattatattt aatttattta attaatactt tatattatag
     2041 attgtgacat tctttagagt tcaaactttt taggtattct ctaacttcca gtaaattttc
     2101 aagtatctta tgtgattgtg atttaatttg ctcatctggc tcttttaaat ctggtacatt
     2161 tatacatctt atcccaccat tgtatgcagc ttcaactccc ataggcgaat cctctatgac
     2221 aatacaattt tggggattaa catttatctt tttagctgct tttaagaata tctcagggtt
     2281 tggtttagag tttacaacat catctccaca aactatagca tcaaagtaat cttttaaatt
     2341 tgccttagct aatctcttta ctgctctttc tctctttgtt gatgttgcaa cagccatttt
     2401 gtatccattt tcctttaaaa aagagattag ttcattgact cctaatttga taggagctcc
     2461 cttcctctcc ataaattcaa tcatattttt agttttttcg tcatataaat ctattattgg
     2521 tacagaacta tcgtatatat ctgtaagacc ttctattatt cctttacgat ttcttcccat
     2581 aactgatgta taaatttcct tagtcatagt atatccatac ttttcaaacg tctccatcca
     2641 aaattcaaga gaaattctct ctgagtcaaa caaaactcca tccatgtcaa aaataattcc
     2701 ttctactttt tgcatacaat tcctccaata ttttttgata taattatatt acacttttta
     2761 tgattaattt tacaaccctc aatttatcca attctaatat tgtaaaattg taattttcaa
     2821 aagttacgct ttggtttact ttaatatttg aaccaagcat ataggatatc cacccaccta
     2881 tactgtcaaa tccatcatgc tctatatcaa gtccaaaata cttattaata tagttgatag
     2941 attcagttcc atcaactaag taagaacctg ctcctatttt cttaatatga gtctcttctt
     3001 tgtcaaattc atcttgaatt tctccaacta tctcttctag tatatcttct attgttacca
     3061 caccagatgt accaccatat tcatccacaa ctatagcaag ttgaagtttt tcctttctta
     3121 ttctctcaag agctttatct atagttaaat tttcagatat atatataata tctctcaata
     3181 tttcttctag ttcaatttta ttttcattta tcttttgatt gtataaatct cttatatgta
     3241 caaaacctaa tatatcatct ttattttttc tgcatactgg atatcttgta acaccttcct
     3301 cttttaaaat agctaatatc ttttcttcag aatcactttc atatatacat accatatcag
     3361 ttctaggaac cattatctct ctaattttct tttcttcaaa ttcaaaaata ttatcaacta
     3421 atctttgctc tgattcatct ataagtccac ttttataact ttcttctact aatagtctta
     3481 tttcgtcacc tgtatggggt tcatcaacct catcagcttg tgaatatcca aatggtttaa
     3541 gaaataaatc agtacttaag ttgaaaaaat atattattgg atatgttaac ttataaaatc
     3601 ctactaataa aaaagatgta ctcaacatta ttttttctgt attataaagt gctaaagctt
     3661 taggaaccaa ctctcctatt acaacctcca tcattgttat aattaaaaaa gaaatggaaa
     3721 ttgatatagt atatataata ctttcactaa gattaaatac acttactatt ggtaaaatta
     3781 actctttaac tgtagattct cccatccaac ctaatgcaag tgaacaaagt gttataccaa
     3841 gttgacatgc agaaagatag gaatttaagt tgtcttttat tattttacat ctttttgcat
     3901 tattattacc ttcagttatc atggtatcaa tcctagaact acgaagtttt accatagcaa
     3961 attcacaagc tacaaaaaaa gcatttatca aaacaagcat tactataagt gttactttta
     4021 ataactcccg cataaagtat tggttcttat aatgtaagaa atctataaga actcaaagtt
     4081 ccccccttat atttataaat ttaatttata atatatattc tatcatattt attaatatat
     4141 cctactttat tacaaataat ttttataaac taaataaccc ctttaatatt tatgactgtc
     4201 tatttgataa atattaaagg ggttatttag ttatcatttt ttatttctaa aaatcttttc
     4261 tcaaatgatt tagttatcta tttatccaat tttttacata atttaatgat taaaaatatt
     4321 aattttttca ctataaagta taaatatttt ttgtataaat attatctgat tattaaaata
     4381 aatattaata ctataaataa aaacgaagca tgatttttat ttaaataaat cccaaacttc
     4441 g
//
LOCUS       DAEPOZ010000262         4376 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.262, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000262 DAEPOZ010000000
VERSION     DAEPOZ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.262"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..409
                     /gene="cas6"
                     /locus_tag="KR440_003301"
     CDS             <1..409
                     /gene="cas6"
                     /locus_tag="KR440_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBH0700305.1"
                     /translation="RFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSN
                     YIECLNYIANLTLESIRGSGLRKPLEFIPLNFKKRVLKEKIRGFKVREYYYINAYAGT
                     FFLKGNMEDLNALYKMGIGYRRTENAGMVDILK"
     gene            414..1889
                     /locus_tag="KR440_003302"
     CDS             414..1889
                     /locus_tag="KR440_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700306.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENQLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATEKQKEGIKKDFKNRRFPYVRNSGKYGLNTSLENMETNVKRIVDTVIKSN
                     IEIDEIDKLDLTQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYRFDIDTNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREP
                     GNTRNLGKKMIDNGNLSNLKELLLDNISVLRQLTGTSREMDFVSSSYNIGLYIKLCWI
                     VDGGEEYKMKNYMESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            1909..2922
                     /locus_tag="KR440_003303"
     CDS             1909..2922
                     /locus_tag="KR440_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HBH0700307.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYREKIHA
                     KTSIATITYDLRRMLHQEYGWNLSNIIFGKKGNIYPSIKQNVGCVDENGLETDVFGYL
                     IPLKDKGSISKASPLRIIPFRSLNSYRGSTQLITNRGFLSSEFGRKYYDEKEENEVLR
                     DENFPTSQALAIEETLSDYYVYTITLELDRIGVVEVEDGKLLLPEERKFMSKELREKA
                     VKDILDAITVFTRNIKHSSVLLKPLAVMGGAFDKVVPFFWDDVDYNADSGEINLEGVI
                     ETIESYSLKESNTILAINDKLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMWY
                     LKE"
     gene            2938..3744
                     /gene="cas5"
                     /locus_tag="KR440_003304"
     CDS             2938..3744
                     /gene="cas5"
                     /locus_tag="KR440_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HBH0700308.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYTNMRKGKDKEKFLLQGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIKKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVELKEVMPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPY
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCNVCIDTKEVFTWL"
     gene            3771..>4376
                     /locus_tag="KR440_003305"
     CDS             3771..>4376
                     /locus_tag="KR440_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HBH0700309.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLLEEDKLYIYKSIMLHHGNYEDYMRLSTSKVQEEIYDYIE
                     KGILEKEEFNLKDIESYINDILNVDFKFGEDVLDYDYIDKLS"
BASE COUNT         1889 a          364 c          777 g         1346 t
ORIGIN      
        1 tagatttaaa ataaaaaata taaaaataca taaagaacac gagataaaag aaaatggtgt
       61 tatatttaaa actatgtctc ctataatagt aaagaataaa gaaggtaaat atttagatgt
      121 agaagattca aattatatag aatgtttaaa ctatatagct aatctaactc tggagagtat
      181 aagaggtagt gggcttagaa agccattgga atttatacct cttaacttca aaaaaagagt
      241 gttaaaagaa aagattcgtg gatttaaagt gagagaatat tactatataa atgcttatgc
      301 aggaacattt tttttaaaag gaaatatgga agatttaaat gcactgtata aaatgggaat
      361 tggatataga aggacggaaa atgcaggaat ggttgatata ttaaagtagc gaggtgagag
      421 aaataaaagc taaattttat agatcaggtg atattgtaag agatattggc attgtttact
      481 tttatgaatt actaatggat ttaaaaaacg aactagaaaa aaatgattat aagctaaaat
      541 ttacttgtga gttaaataga aattatttat ctttagaatc tgcagaatat atagacccta
      601 agtatatatc tgattatata ttagaaaatc aactttttaa ggtatttaaa aatgggaaaa
      661 aggaaacctt agaaaaaata tttagtaata tagataattt aacgtatgta aattttattg
      721 aagaattaga taaatcttct gcaactgaga aacaaaagga agggattaaa aaagatttta
      781 aaaacagaag atttccatat gttagaaata gtggtaagta tggattaaac acgagtttag
      841 aaaatatgga aacaaatgta aaaagaatag tagatactgt aattaaatca aatatagaga
      901 tagatgaaat agataaactt gatttgacac aatatgaaga aaaagatagt gtatgtaatg
      961 tttgtaatat aaataaaaca acaaaactag atattgattt aagagagcga aaggactcta
     1021 agtataactt tttatttaga ggtgcagaaa aatcaggctt taaaagaagt ggacaagttg
     1081 aaagtaatat ttgttttgag tgtgaatttt tcaacttaat gtgtctttta tatataaatt
     1141 taaaaagacc aatggttttt gcatatacga atgatttaag ggaattagca tttttaaatc
     1201 ataagataat gcttaaaaga aagatgtatt cagataaatc attttataaa aaacttttgc
     1261 atgaaaaaat aagttcgatt agactatata ggtttgatat tgatacgaat aaaggtataa
     1321 ttctaaagtt tgactctatt atagagtata aagaattatt aaaaaaaatt gaacttatag
     1381 atattataga taattataat ttttcaagag agccagggaa tactagaaat ttgggtaaaa
     1441 aaatgattga taatgggaat ttatcaaatt taaaagaatt attacttgat aatataagtg
     1501 ttttaaggca actaactgga acttctcgag aaatggattt tgtatctagc tcatataata
     1561 tcggtttata tataaaattg tgttggattg tagatggagg ggaggagtat aaaatgaaaa
     1621 actatatgga aagtaattta atatattcaa gagtaggtaa agacttattt aataaaatga
     1681 cagatgaatc cagaagaaaa aatttttcta tgaaagtgat tcaactactt aagtctaatg
     1741 atagaactca gctatttcaa actataatgc atgtgttagt ttctaatgga atcttaatag
     1801 gagaagggtt tgtagaaggt ctaatgcaga gttctgaatt agaattaaat acgaatgtag
     1861 gtctatttat tcaagaaatc atgaaataaa aaagggggaa aatttaaaat gaatgaaaaa
     1921 agagtaaaag gagtaacatt aactatactg acagaaagcc cagttccact ttcatatgac
     1981 caagggtatg gaaattatac accaataaaa aaagaacaat atagagaaaa aatacatgca
     2041 aaaactagta tagccacaat aacatatgat ttaagaagaa tgctccacca agaatatggt
     2101 tggaatttaa gtaatattat ttttgggaaa aaaggaaata tatatccatc tattaagcaa
     2161 aatgtaggat gtgtagatga aaatggatta gagactgatg tatttggtta tttgatacca
     2221 cttaaagata aaggttctat aagtaaggct tctccgctta gaataatacc ttttaggtca
     2281 ttaaattcat atagaggttc aacacagttg ataacaaata gaggtttttt aagtagcgaa
     2341 tttggtagaa aatattatga tgagaaggaa gaaaatgaag tacttagaga tgaaaacttt
     2401 ccaacatcac aagcattagc aatagaagag actttatcag attactacgt ttatacaata
     2461 acattggagc ttgatagaat aggagtagta gaagtagaag atggaaagtt gcttttacca
     2521 gaagaaagaa aatttatgag taaggagtta agagaaaaag cagtaaaaga tatattagat
     2581 gcaattactg tgtttacaag aaacataaaa cattcatcag tactgttaaa accacttgca
     2641 gtaatgggtg gagcttttga taaagttgta ccgttttttt gggatgatgt agattataat
     2701 gctgatagtg gtgagataaa tttagaggga gtcatagaaa ctatagaaag ttatagcctt
     2761 aaagaaagta acacaatact tgctatcaat gacaaattaa aaatttctaa taaaaaggaa
     2821 ttaaataagt ttaatttaag taaataccct gtaaaagaga ttaaaaatct agcagataga
     2881 ttggaaattg gagaagataa tatgtggtat ttaaaagagt aggaggtttt ttgaattatg
     2941 aaagtattac aatgtaaact taagcaacca actgctcact atagagaccc aaaagtattt
     3001 caaaatgaat atataagtac tttaaattta ccatctaaga ccacaataat gggaatgata
     3061 acgtatttat gtgatagaag attaaactct gatatagata taggaataat tggaacacat
     3121 catcatagag aactagaatt ttcaagagga gagaacattg atttttggaa tgaatataca
     3181 aatatgagaa aaggcaaaga taaagaaaag tttttgcttc aaggaaacta ctatgattac
     3241 tataaagaac ataaagctca aaatagcata ttaaactatg aagttcttaa agaagtagaa
     3301 ctgactatat ttatttcttg caaggatgat gaagaacttg aatttattaa aaaaaagctt
     3361 gaaagtccat gtaaatatgc aaatttagga agaaaagaag attttgtaat accaagtgaa
     3421 aaagggtgtt ttgtgaaaga agttgagtta aaagaggtta tgcctatgaa tactcgtgat
     3481 gctataaaag aaaatataaa attaaagaat acatatgtaa gagttgactt aagagataaa
     3541 gagaatgtcg agactataat aaatcaaggt gtattgattg cattacctta taaatataaa
     3601 gatttagaag caaatagaga tgatagagtt tatgagtttt gccattatat atatgttgat
     3661 aatgatggta tatatcctaa aaatattaag gtaaatgtat gtaatgtatg tattgataca
     3721 aaagaagttt ttacatggct ttaaattatt ttgaaagata ggtgtttatc atgaaaaata
     3781 tcatgcaaaa agaaatatta gctaagtcat ataatttaag aactaatcaa caagaacaaa
     3841 cattagttga acatattaag gatttatttg aagtattaga atctatatta gaactaaatc
     3901 tatattcaga taaagatgta gagatactaa aaatatgttg tgctttgcac gatttaggaa
     3961 aaattaattc aattatgcaa caaaaaatgg agattaataa taaaatatcg tatagttgct
     4021 cagaagaaga gaggaaaaag ttagagtctg acaaaaaatc tttaaaaaag atagcaagac
     4081 acaatatatt ttctggagca tttttaaaag atatattaga aaaaatgaac ttgttagaag
     4141 aagataagtt atatatatat aaaagtataa tgttacatca tggaaactat gaagattata
     4201 tgaggttaag tactagtaaa gttcaagaag aaatttatga ttatattgaa aaaggcattt
     4261 tagaaaaaga agagtttaat ctgaaagata tagaaagtta tataaatgac attttaaatg
     4321 tagattttaa atttggtgaa gatgtattgg attatgatta catagataaa ttaagt
//
LOCUS       DAEPOZ010000263         4374 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.263, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000263 DAEPOZ010000000
VERSION     DAEPOZ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.263"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..544
                     /locus_tag="KR440_003306"
     CDS             <1..544
                     /locus_tag="KR440_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBH0700310.1"
                     /translation="HVNHSTKKNILIMGGGLGLLPKSEQFYKELNSLEGVKTTVITGN
                     NKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETIYSELPILAFNP
                     FLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSSNMKELKKQFDN
                     NTLENILFSLDEQGACRECM"
     gene            535..1233
                     /locus_tag="KR440_003307"
     CDS             535..1233
                     /locus_tag="KR440_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBH0700311.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            1236..2300
                     /locus_tag="KR440_003308"
     CDS             1236..2300
                     /locus_tag="KR440_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBH0700312.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            2483..3187
                     /locus_tag="KR440_003309"
     CDS             2483..3187
                     /locus_tag="KR440_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0700313.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            3180..>4374
                     /locus_tag="KR440_003310"
     CDS             3180..>4374
                     /locus_tag="KR440_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0700314.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKN"
BASE COUNT         1714 a          447 c          750 g         1463 t
ORIGIN      
        1 acatgtaaat cattcaacta aaaaaaatat tcttataatg ggtggaggat taggattact
       61 gcctaaatca gaacaatttt acaaagaatt aaatagttta gaaggagtaa aaactactgt
      121 aataactggt aataataaaa aaatgtacta taaactatat ggtagatatg aaaatataga
      181 ggtagtaggt tacaccaatg aagtatataa atatatgaaa gattctgacc ttattatttc
      241 taaaccaggt ggaataacac tatttgaaac aatttattca gaacttccaa tattggcatt
      301 taatccattt ttacaacaag agatagataa tgctagtttt atattaaata atgaaattgg
      361 gagaatactt ggtaaaaata aaaaatacta tgtagatgag ataaaagatt taatttatga
      421 tgatgctact cttaaagaaa tgagcagtaa catgaaagaa ttaaaaaaac aatttgacaa
      481 taatacctta gagaatattt tattttcact tgatgaacaa ggagcgtgta gagaatgtat
      541 gtagtaggtt taattatcgt tgttgcattg atttttttag tccattcaat tatacctaca
      601 tactataata aactattaaa taaagaggta ttaaaaaata tggctggaga aaatgaaatt
      661 gcacttacat ttgatgatgg tccagataag agatatacag aaaaattact agatgtgtta
      721 aaagaaaatg atatacaagc tatgtttttt gtagtagcaa agaatgctga aaaagagcct
      781 gaaattataa aaagaatgtt gagagaaaat catatagttg gtttacattc attggaacac
      841 agaaatgcat ggctttatag ttatagttat gttaaaaaag attttattga aagtacaaat
      901 ataatgaaaa atttaggtgt agatgtaaac tactatcgtc caccatgggg tcatactaat
      961 atattttcaa attcatttgt taaaaaatat aatctaaaaa tgactttgtg ggatgtaatg
     1021 gcagaagatt gggagaaaga tagtacagta gacattatta taaacaagtt aatgagtaga
     1081 acaaaagaaa attctataat ttgtttacat gatgcaggtg aaaattcagg tggtgcagta
     1141 ggtgcacctg agcgtactat tgaagcttta aagatagcta ttccaaaact taaagccagt
     1201 ggtttaaagt tcgtaacacc agaaaggatg tagttatgat aagtaaaact gaaaagaaaa
     1261 ataaatgcct aggtagtgta gcttttttag ttttactaat gggcataaca ggatattttg
     1321 tttttagagg gcagtcagtt gaatcattga taaaatcatt aaaaggtgca agccctatgt
     1381 ttatactaat tggatttgca atgatgttta tatatgttgc ctgtgaaggt ataaatatat
     1441 acttaggtat gaaagcttta aaccaaaaaa ctactctttt aaaatgtatg ggatatgctt
     1501 ttattggatt ttattttagt tctattacgc cttcagcatc aggtggtcaa ccagcacaag
     1561 tttattatat gaaaaaagac gatataaata tatcatattc ttcacttata ctattagtca
     1621 ttgttgttat tcaccaagta gttatactgg catatagtgg aataatgttc attatggaac
     1681 gtgagtttat attaaataat gtaagtggta tgaacatttt actaatatat ggtgtaataa
     1741 caaatgttgc tcttgtcatt ggagtaattg caattatatt ttcaaaaaaa cttgtaaaca
     1801 attttataat atcaataaca aatttattag gtaagttaag aataataaaa gatgttgaaa
     1861 gttcaagaaa agttataata tctcaaattg aagagtatgt taaaggtgct caatatataa
     1921 agcagaatcc aaaacttgta gtccaaattt tagtcataac tattgtacaa ataacagcta
     1981 tgtttttagt tcctttcttt gtatacaaag cttttcatct atcaacatat actgtatttg
     2041 agatattagc gatacaatcc ttgcttaata ttgctgtttc ttcattacca ttgccagggg
     2101 ctgttggagc ttctgaaaat agttttatga cattatttaa gatatttttc cctggtcact
     2161 tattagttcc ggcaatgtta cttagtaggg gaataagttt ttatgcattt gtagctataa
     2221 gtggattgat atgtatagta gtacatgtaa aatcttctga aaaagtaaaa gcagtgaaaa
     2281 aagttgctta tgtaagataa tattatatag attattttta ttaatatgtc aatttgtgtg
     2341 tacattttaa ttaagtttta aacatgttta caggagtata aactattaaa acgtgtgaca
     2401 ttgctaagct tttgctaata aagtataata ttttatatag tttgaaatat ttaagaaata
     2461 tagcaaaaaa aggagcttgg tgatgaataa agagattcca aaaaaaattt tagtagctga
     2521 tgataatcct gagattaggg aaatcgtaga aatactttta actggtgaag gttatgaagt
     2581 tattatggca acgaatggag aagaagctgt aaacttagta gatagtacga ttgatttgat
     2641 tattttggat gttgtaatgc ctgtaaaaac aggatttgtt gcatgtaatg aaattagaga
     2701 aaaaacgaca gcaccaatat tatttttaac tgcaaaaact caagactctg ataaagttat
     2761 tggattttca gcaggaggag atgactattt atcaaagcct ttttcatatt ctgaactaat
     2821 ttcaagggtt aagtcgctac ttagacgtta ttatatatac cagggaaagg aaaaggaaag
     2881 agagccaaat atattaaaaa caaaagagtt atgtgtcaat ttagatactc aagatgttac
     2941 actaaatgga gaacctgtgt ttttgacaac tatagaatat agtatattaa ttcttatgtt
     3001 gaaaaataga aaaaaagttt tttcgtctga aaatttatat gaaactattt gggatgagca
     3061 gtatttttat acggcaaata atacaataat ggttcatatt agaaatttaa gaaaaaagtt
     3121 agaaaaagac caaaaaaatc cacaatatat aaaaaccgca tggggaaagg ggtattatat
     3181 tgattaacaa aattaagcca tctaagttgg gagttatact attgatatat gtagtaatct
     3241 cagctgcagt ggctttcatt ttaaatatac cattttactc tgctgttgaa tctatagtaa
     3301 aagatgtatc agaagggaaa gtatatttta aaagagaagt aaatcaactt atggataatt
     3361 ttcaaactta tgtgaataac aatggtataa gagcagatga tggagatgat atagcaaaat
     3421 ggaatagtga taattggttt gtttctatga agatatataa aggaaataag gttttttatg
     3481 atacattata ctatcctaat gaagtacctg ataagagtga ggaaaatgag ttttatagca
     3541 gtcccatggc tagaaactat gagatacaat tttctgatat taaagctaat atagttgtag
     3601 tagctttatt tagatttaga tttcaaaatt ttatagaagc aatttctatt acatttatgg
     3661 cacttacatt tattataaca tttttactgt tgtttagtag aaagataaag tacctaagga
     3721 aaatagaaat gggtataaag atagtagaga gtggcagttt aaagtataga atcccagtaa
     3781 aaggtaatga tgaattatca tcacttgcat ctagtattaa tgagatgagt catacattgg
     3841 agaatcaact tataaaagaa agggagatga agaataaaaa taaagatata gtaacttcga
     3901 tatcacatga tgttagaact ccattaacat cagtaatttg ttatttagat ttgatcaaag
     3961 accataaata caatagtcct gagcaattgg aacaatacat aaaaaatgta aggtctaaag
     4021 catatcaaat aaaagattta acagatgacc tttttacgca ttttgttaat tctggagagg
     4081 aagtttcaaa agggtatgaa aaaataaatg ctaatgaatt tatttttcaa ttacttttag
     4141 atagttcata tacattggag gagaaaggat ttcatataac aatagaaaat gaactttata
     4201 accaatatca tatttatgta gatgttacac aatttagaag gatatttgat aatttatgtt
     4261 ctaatataat aaaatatgct aggaaagata agcctatagt atttttaatt aaagtagagg
     4321 attctaaatt actaattata caaaaaaata gagtaagtaa tttttctaag aatg
//
LOCUS       DAEPOZ010000264         4315 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.264, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000264 DAEPOZ010000000
VERSION     DAEPOZ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.264"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     repeat_region   1..888
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=133..161
                     /rpt_unit_seq="atttacataccacatagttaatataaaac"
     gene            1535..2806
                     /locus_tag="KR440_003311"
     CDS             1535..2806
                     /locus_tag="KR440_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBH0700315.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIILEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSIADTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPM
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
     gene            complement(3445..4200)
                     /locus_tag="KR440_003312"
     CDS             complement(3445..4200)
                     /locus_tag="KR440_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HBH0700316.1"
                     /translation="MAHICFEDICYLLLKEAMKLKLIPHNKIVLFEDDLSIGNIFDVN
                     NYELRENIFKIRNVFENNPKLLFKPDLLEDRKLSKCRNIIIWCANNPKDLCGVYYVVS
                     KFQDKNIKIALVDEKKTEDALIRYSFTSEMSPEDFPFFLDKTLEIGFNEKLEFKNKWD
                     VLVKENSIFRALVGNEIKSVEETYFDKYILRNISDKWQPLIEVVANCMFEDEMRVKDW
                     FILWRLEKMASLKIVKIKGSKGDFYSDKEIRKV"
BASE COUNT         1643 a          609 c          472 g         1591 t
ORIGIN      
        1 ttatatatca tgtagttaat ataaagcata tttgtgtcga tatatagttc taactaagta
       61 taagatttac atacctcata gttaatataa aacaatgtgt ccgaattgga cacatagaaa
      121 ggaaaagtaa aaatttacat accacatagt taatataaaa ctatgcttat atattatgtt
      181 tattctgttg tttctaaaat ttacatacca catagttaat ataaaacgat tttttcatgc
      241 ggaggaaaat tgatgtgggg acatatttac ataccacata gttaatataa aacagataat
      301 ctaggcacac atacaatgcg caaaacttta tttacatacc acatagttaa tataaaacat
      361 tcagacgata taggcgcagg agctaagtat tctgaaattt acataccaca tagttaatat
      421 aaaaccagca cgccaagtat aagtaaaact tccactatca ccatttacat accacatagt
      481 taatataaaa ctaaatgttg ctccgtaacc taaaaacata atgctccaat ttacatacca
      541 catagttaat ataaaacgtg aaagaaaaat taatatgcta tttacatttt acaaatttac
      601 ataccacata gttaatataa aaccattcca caatttatac aattttttct gttctaaaac
      661 atttacatac cacatagtta atataaaact cttcttatct tccagttgca aaatctatgc
      721 ttgacctatt tacataccac atagttaata taaaactagt ttgagtggta aaagtacgaa
      781 gtacccctct ttaatttaca taccacatag ttaatataaa acatatgtaa ttcaccgttt
      841 taaattatag ataaattaaa tttacatacc acatagttaa tataaaaccc caaaataaac
      901 taagtatttt cagtacttac acacatacaa ctctctcaaa tttgcagtga accatgagta
      961 gtgcaattga taacatttat catacaccct caatccctta cattgcaact gttaaaccct
     1021 actttatcaa aaatatcgct cactgcaaaa tttatatatt tttattatat cataaaattc
     1081 aaactataaa attaaaatgg aataatttat caatttaaga acacctacat atttgtaaat
     1141 acttactata ttatctattc taataattct tcttctagct ggaactaata taaaaatcat
     1201 ctctgaaaga ttaggacata ctaatataaa aataactatg aatagatatt ttcttgtttt
     1261 aaacgatatg gataaaaaag cttttgaaaa tataatcaaa gttctcttta aataacattc
     1321 cattttatgt aagtaagcat tatatttttc ttaaatgtca cttaagtgtc agttatatat
     1381 aataatagta aacaactgta tttcactaat atttgctatt atctaatagt atttatagtt
     1441 tagatttgat ataatacatg tggtaaaatt ttgatttgta aaacaaaatt ttacctacat
     1501 atattaaaac aaatttaaaa ctaaggagac ctatatgtta aaagtattaa aaggaaatat
     1561 aatttttaca aaaacttcag aaactttcac agtatttgaa aatagttata ttatactgga
     1621 aaaaggtaaa gtaaagggaa tatttaaaga gatacctaag gaatacaaaa acctaaaagt
     1681 agtagattac actgataagc ttataatacc tggtatgaat gacctccatg cccatgcctc
     1741 tcaatttaaa aatcttggaa tggctatgga taaagagctt ttgccatggc ttgaaactta
     1801 tacatttcca gaagagtcaa attataaaga tatagaatat gcaacaaaaa tgtataaaaa
     1861 atttgtaaaa gaactttgga aatctggaac tacaagaata gcagtgtttg caacagtaca
     1921 taaagaagcc tctatgaaac ttatggatat attcaaagaa gttggtcttg gagcatacgt
     1981 tggtaaagta aatatgaata tgaattgccc tgatacttta ttagaaaaca ctcaggattc
     2041 tattgctgat acagaagaaa ttataaataa gtatagtgat acagattcta ttgtaaaacc
     2101 aataataaca cctagattta taccaagctg tacaagtgaa cttttaaaag gtttaggaga
     2161 tttatgttta aaatataata ttcctatgca atcacactta tctgaaaatt atgatgagat
     2221 tgaatgggtt agaagtctag aacctgaatc taaattctat ggagatgcat ataatagata
     2281 taatttattt ggtcaaactc ctactttaat ggctcattgt gttcattgtt ctcaggatga
     2341 aatagattta atgagagaaa acaatgttat ggcagttcac tgtccagctt ctaactttaa
     2401 tgttggtagt ggtgctatgc ctatcagaaa attaatcgat aataatatta gattagcact
     2461 tggatcagat ataagcggtg gacatacatt gtcaatattt aaagcaatgg tatctgcaat
     2521 acaattatct aaattatatt gggttaattc tggtaagaag tataatttct tatctttatc
     2581 tgaggctttt tatattgcaa ccaaaagtgg tggaagtttc tttggaaaag ttggtagctt
     2641 tgaagaaggt tatgattttg atgcattgat tattgatgat tcaaatttaa atcatgacaa
     2701 ttattctata cttgaaagac ttgaaagatt tatctatgtt ggtgatgata gaaatatcat
     2761 tcatagatat gtatgtggaa aactaattga agaacctaat atataattat aataaaaatt
     2821 ttaattatat attatggtta atttatgcaa ctataattaa taaaagagta tattttaaat
     2881 ctaaaagcta taggttgaaa catactcttt ttattttgtt tatttatagc tttagttatt
     2941 aatcatatat aaatctacaa aacgtattcc tagtaactta tattttttga tatctacaat
     3001 cataagacta tattattgga ttcatacctc aaaaatattg ccttctgaac attattatat
     3061 tcaaagttat gttaatttac aatgatatat ctaacatgta ttgattaaaa aaaattaaaa
     3121 cttgcttttc atctcctata tctaaagaag tggcatttag aataaactat tgtaatatta
     3181 aagttgaaat tttctagttt atttactcta tagcaccata ttttaatact attaagtaaa
     3241 aatgtagtta aaatatatac taatatccct gaaataagta tttctttttt atttagcaca
     3301 cttattcgta aggtattagt tattatattt tcaaattata gaataaatat tatacttatt
     3361 gctaattata atattaatac tgttaagata tttttcttca tagttatacc cattatttaa
     3421 tacaatttat tataaattca atttttaaac ttttcttatc tccttatcag aataaaaatc
     3481 ccctttactt ccttttattt ttactatttt taaacttgcc atcttctcta atctccaaag
     3541 tataaaccaa tcttttacac gcatttcatc ttcaaacata cagtttgcaa ccacttcaat
     3601 taaaggttgc catttgtcac tgatatttct taatatatat ttatcaaaat aagtttcttc
     3661 aacactttta atttcgttac ctactaatgc tctaaaaata gaattttctt taactaacac
     3721 atcccattta tttttaaatt ctagtttttc attaaagcca atttctagtg tcttatctaa
     3781 aaagaatgga aaatcttctg gagacatttc actggtaaat gaatatctaa ttagagcatc
     3841 ttctgtcttt ttttcatcta ctaatgcaat ctttatattt ttatcttgaa acttactcac
     3901 tacataatat actccacaca aatcttttgg gttatttgca caccatatta ttatatttct
     3961 acattttgat aatttcctat cttctaacaa atctggcttg aataatagtt tcggattatt
     4021 ttcaaaaaca tttctaatct taaaaatatt ttctcttaat tcgtagttat ttacatcaaa
     4081 aatattacct attgataaat catcttcaaa taatacaatt ttattatgtg gtattaactt
     4141 taacttcatt gcttctttta ataacaaata acaaatatcc tcaaagcata tatgtgccat
     4201 aaaattcacc tcttttaaaa ttaacaattt attttattat aatatataaa ctgtaattat
     4261 caaacacttc taatctccta tattttcata ttaatgtaat atctttttta ttaat
//
LOCUS       DAEPOZ010000265         4301 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.265, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000265 DAEPOZ010000000
VERSION     DAEPOZ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.265"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..1650)
                     /locus_tag="KR440_003313"
     CDS             complement(<1..1650)
                     /locus_tag="KR440_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700317.1"
                     /translation="MPVINKYKIINFKFGMGQERILSNRTMELYGENLQVEAENTAGK
                     TMSIQTIIQSICPLSDVAKPFTAIYNKKEPLYSMTEWLLDDGKTLLLTGIGFEKKAGS
                     SKEEGKEKKDDLYKYFMFIIEESKEMEVDIDNIPLLKNNDKGIRIVQPLTASQDYIKM
                     LRDKYGKNKVHFFNNYLRKQHREKLEEYGINQSEWKEIIIKLNSNNKEGGLSDFVKEH
                     NTSEKLIRDKILPLIETSLTTEEFAPILQIKEQVRKCINNIIDMNEKLSDYKNYRDLN
                     IRVESLQNKINRILFIEEDKNNVLGELANLYVHIENKRNELNEEIKQYQKMKASKEEE
                     FKIVEYEEDSYEYFRKEKEIQDTQVEFDKAKNELNDKIEELELDEQYLNISKFKRKKK
                     GLEEGEKDKIEKESEKQRKEVNQEEITAIINRLGSSIHYCLKEESIINNEKLKENTNN
                     NIAIQEKKNQLNDDKSKLTKVLISKNSEIKYIEGKERDFNNQLHEFKLNNTDIKGFIS
                     NNFLGEVIDYTRYINYLNIKVKSIEENLESKVMQIEESNKLK"
     gene            complement(1637..2365)
                     /locus_tag="KR440_003314"
     CDS             complement(1637..2365)
                     /locus_tag="KR440_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700318.1"
                     /translation="MNISLNDYKIANEIFYRLLKKSELKEKEAKELFLAYINNSNVRY
                     ALEAICEVSKIKIILISDTLYLIPNNDNDIFGFDYRRESLLGKDLKDSYLGYLIMTII
                     FSEFTNGVAPASYIQVLNLLDLVQESLERSIARDNLEEIEKSCEFNITILKNYWESKR
                     KWDESSKKGIETTSADFQIGFIRKIINFLKNEGLIVHIEDEDKIKPTRRFLDLMNNYF
                     LDEERKVLIEKLVLRKGESINASN"
     gene            complement(2383..4002)
                     /locus_tag="KR440_003315"
     CDS             complement(2383..4002)
                     /locus_tag="KR440_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700319.1"
                     /translation="MEMINILEGYNKRVNCIGSYYLIATLANNRNKFKEFDNIQFYNL
                     LIQVLCYIFDRSLRRKNCLRDDIKDFIEEINRMDYKIMLSEDDLKDLANYIINGLTNS
                     GKVYLFTYYSLEQEKHIDESIKIIEDKNVKINNQERLSYSLTTEGYRLLLSTKEYDEL
                     FQIQISQMIAKLRIEKGDYQGAKEDIYDIINTLEIHQQKIDNYIKIIKVNILYIKEEK
                     YSTVIDTTVETLLEEMDKYEELKKEVKLLIKDKEDYLEKDEDIDKEKIKNIRTQLLQM
                     KELIDGIVLAKSTATNLLSRVQDFREEFKEILKKLLTTPVIKRFNFKEEILDRLEKDS
                     DFLLIIRELYISLFKVELPNRFNIGVAYQEQKLLNTREIENEIVNKDELEIIENKEKE
                     ERINYCENYYFDFFKYLIKYGLEKGQFTLEEFLKNYLKYDKVIYLEITKDSKLMRDII
                     IYFANQRESINIKKIIRMLENSAFSPNTDFQIERLIERLLVEDFELLNSVSHIKSNKI
                     FEDEMKINTEINLETMTAVKLEIANIKFEII"
     gene            complement(4002..>4301)
                     /locus_tag="KR440_003316"
     CDS             complement(4002..>4301)
                     /locus_tag="KR440_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700320.1"
                     /translation="NEFLYWGDIDKTGLSIYTSLKDTNEINNIRLFEVAYEHMIDKTK
                     ENTLRNIPKNQKDNFECGLENINNLNLKESIKNILVQGKYIPQEALNRFDLEEYN"
BASE COUNT         1214 a          666 c          399 g         2022 t
ORIGIN      
        1 cttaagttta ttcgattctt ctatttgcat gactttcgat tcaagatttt cttctattga
       61 ttttactttt atatttaaat aatttatata cctagtataa tctattactt cgcctaaaaa
      121 attattagaa ataaagcctt taatatcagt gttattaagt ttaaattcat gtagttgatt
      181 attaaaatca cgttctttgc cttctatata tttaatttct gaatttttac ttatcaatac
      241 ttttgtgagc ttacttttgt catcattaag ttgattcttt ttttcttgta tagctatatt
      301 gttgtttgta ttttctttaa gtttttcatt atttataata ctttcttcct ttaaacaata
      361 atgaatactg ctacctagac gatttataat agccgttatt tcttcttgat ttacctcttt
      421 tctttgtttt tcactttctt tttctatctt atctttttcc ccttcttcta gccccttctt
      481 ttttcttttg aactttgata tatttaaata ttgttcatct aattctagtt cttctatttt
      541 atcatttaat tcatttttgg ctttatcaaa ttctacctga gtatcttgta tctctttttc
      601 ttttctaaaa tactcatagc tatcttcttc atattctact atcttaaatt cctcttcttt
      661 tgatgctttc attttttgat actgttttat ttcttcattt aattcattgc gtttattttc
      721 tatatgtaca tatagattag ctaattctcc taatacatta tttttatctt cttctatgaa
      781 taggatccta tttattttat tttgtaaact ttcaactcta atgtttaaat ctctatagtt
      841 tttataatct gataattttt cattcatgtc tataatatta tttatacact ttcttacttg
      901 ttccttaatt tgcaatattg gagcgaattc ttctgttgtt aagcttgtct ctattaaagg
      961 taatatttta tctcttataa gtttttctga agtattatgt tctttaacaa agtctgatag
     1021 tccaccttct ttgttattac tattaagttt tataataatt tctttccatt cactttggtt
     1081 aattccatat tcttctaatt tttctctatg ttgttttctt aagtaattat taaagaagtg
     1141 tactttgttt tttccatatt tatctcttag catttttata tagtcttgtg aggccgttaa
     1201 aggttgtact atccttatac ctttgtcatt gttttttaat aaaggtatat tatctatatc
     1261 aacctccatt tctttagatt cttctataat aaacataaag tatttgtata aatcgtcctt
     1321 tttttctttc ccttcttctt tagatgatcc agcctttttc tcaaatccta ttcccgttaa
     1381 taataacgtt ttaccatcat ctagcaacca ttcagtcatt gagtataatg gttctttttt
     1441 attatatatt gcagtaaacg gtttggctac atccgatagt ggacaaatac tttgtatgat
     1501 agtttgaata ctcatagttt tgcctgctgt attttctgct tctacttgta agttttctcc
     1561 atacagttcc atggttctgt tgctaagtat tctttcttgt cccataccaa atttaaaatt
     1621 tataattttg tatttattaa ttactggcat taatactttc cccttttctt aaaactagtt
     1681 tttctattaa tactttcctt tcttcatcta aaaaataatt attcataaga tcaagaaatc
     1741 ttctagttgg ttttatctta tcttcatcct caatgtgtac aattaagcct tcatttttta
     1801 agaaattaat tatttttcta ataaacccta tttggaaatc tgctgatgtt gtttctattc
     1861 ctttcttact agattcgtcc cactttcttt tactttccca atagttcttt aagatagtta
     1921 tattaaactc acatgatttt tctatttctt ctagattatc acgagctatt gaacgttcta
     1981 aggattcttg tactaaatcc agtaagttta atacttgaat atatgatgct ggtgctaccc
     2041 catttgtaaa ttcagaaaat attattgtca ttatcaaata tcctaaataa ctatctttta
     2101 aatctttccc taataaagat tctcttctat agtcaaaccc aaaaatatca ttgtcattat
     2161 ttggtattaa gtataaggta tctgaaatta atattatttt tattttagat acttcacaaa
     2221 ttgcctctaa agcatacctt acattactgt tatttatata agctaaaaat aattctttag
     2281 cttctttttc ttttaactca ctttttttaa gtaatctata aaatatttca ttggctattt
     2341 tatagtcatt taaagatata ttcattgtat ttccccttat atttagatta tttcaaattt
     2401 tatatttgct atttctagct taactgcagt catagtttct aaatttattt cagtatttat
     2461 tttcatttca tcttcaaata ttttattact tttaatatgg ctaacgctat ttaatagttc
     2521 aaaatcttct actaataaac gttctattaa tctttcaatt tgaaaatctg tatttggtga
     2581 gaaggcacta ttttctaaca ttctgattat ctttttgata ttgatacttt ccctttgatt
     2641 agcgaagtaa attattatat ctctcatcaa tttggagtct ttagttattt ctaagtaaat
     2701 aactttgtca tatttcagat aattttttag aaattcttct aatgtaaatt gtcctttttc
     2761 aagtccatat ttaattaagt atttaaaaaa atcaaaatag taattttcac aataatttat
     2821 acgttcctcc ttctctttat tttcaataat ttctaactcg tctttattaa ctatttcatt
     2881 ttcaatttct ctagtattta atagtttctg ttcttgatat gccactccaa tattaaatcg
     2941 atttggcaac tctactttaa ataaagatat atataattct cttattatca ataaaaaatc
     3001 actgtctttt tcaagtctat ctaagatttc ttccttgaaa ttgaatcttt taattactgg
     3061 agttgttaat agttttttaa gtatttcttt aaattcttct ctaaaatctt gaactctaga
     3121 taacaaattt gtggctgtag actttgctaa aactattcca tctattaatt ctttcatttg
     3181 taatagttga gttcttatat tttttatctt ttctttgtct atatcctcat ccttttccaa
     3241 gtagtcctct ttatctttga ttaatagttt tacttctttt tttaattctt catatttgtc
     3301 catttcttct aaaagtgttt ctacagttgt atctattact gttgaatatt tttcttcttt
     3361 tatatatagt atatttactt ttattatttt tatataatta tcaattttct gttgatgaat
     3421 ctctaaggta tttattatat cgtaaatatc ttcctttgcg ccttgataat cacccttttc
     3481 tatccttaac tttgcaatca tttgtgatat ttgaatttgg aatagttcat catattcttt
     3541 tgttgataac aacaatctat aaccctctgt tgttaaggag taggatagtc tttcctgatt
     3601 atttattttt acatttttat cttctataat tttaatactt tcatctatat gtttttcttg
     3661 ttctaaagaa tagtaggtaa atagatatac tttaccacta ttggttaatc cattaattat
     3721 atagtttgct aaatccttta agtcatcctc tgaaagcatt attttataat ccatacgatt
     3781 tatttcttca ataaaatcct taatatcatc tcttaaacag ttctttcttc taagagatct
     3841 atcaaatata taacacaaaa cttgaattaa taaattgtaa aattgaatgt tgtcaaattc
     3901 tttaaattta tttctattgt tagcaagagt tgcaataaga taataagatc ctatacagtt
     3961 tactctctta ttgtagcctt ctaaaatatt aatcatttcc attagttata ttcctccaag
     4021 tcgaatctat ttagagcttc ttgaggtatg tattttcctt gtactaaaat atttttaata
     4081 ctttctttta agtttaggtt attaatattt tctaatccac attcaaaatt atctttttga
     4141 tttttgggga tattcctcaa ggtattttcc tttgttttat ctatcatatg ctcataagca
     4201 acttcaaata atcttatatt attaatttca ttggtatctt ttagggaagt gtatattgat
     4261 aatccagttt tatctatatc tccccaatat aaaaattcat t
//
LOCUS       DAEPOZ010000266         4296 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.266, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000266 DAEPOZ010000000
VERSION     DAEPOZ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.266"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(15..779)
                     /locus_tag="KR440_003317"
     CDS             complement(15..779)
                     /locus_tag="KR440_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HBH0700321.1"
                     /translation="MQIIDININNIDSRHICCSISEKKGENCVFSKKAWMKNNFDKGL
                     AFKRFDVRGKAFIEYIPAENAWCPITADNYIFINCFWISGKFKGQGYSNLLLQECVDD
                     AKSKGKYGLVVLSSSKKMPFLSDAKYLKYKGFCLADTSEPNYELLYFPFTDNAPIPCF
                     KNCVKKRKLNENGIVLYYSNQCPHTEKYAPILKKIANSRGIDVNLIKFETAKEAQNAP
                     APFTTYSLFFNGEFVTNEILSEKKIEKFLDTNIKKI"
     gene            1082..1555
                     /locus_tag="KR440_003318"
     CDS             1082..1555
                     /locus_tag="KR440_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0700322.1"
                     /translation="MFKEYLIRELKEDDIKNALDLIWNVFEEFESPDYCDEGITTFHQ
                     FIEYNSIIEKIKEKTLFFWGCFDSKKLIGVLAIRNLNHICMLFVDKNYHKHGVAKKLM
                     TTGIQYCICNKFNLTEITVNSSPYAVEVYHKLGFEDIAVEQIVDGIRFTPMKLLI"
     gene            complement(1924..2481)
                     /locus_tag="KR440_003319"
     CDS             complement(1924..2481)
                     /locus_tag="KR440_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0700323.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKATIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLL
                     SKEEAIKGLADSIDTVLLGIHKKYD"
     gene            2739..3437
                     /locus_tag="KR440_003320"
     CDS             2739..3437
                     /locus_tag="KR440_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0700324.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHEYQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTILENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
BASE COUNT         1605 a          553 c          468 g         1670 t
ORIGIN      
        1 acatctattt ttagttaaat ttttttaatg ttggtatcta aaaatttctc aatctttttc
       61 tcagaaagta tctcgttggt tacaaattca ccattaaaaa aaagactgta tgtagtaaat
      121 ggtgcaggag cattttgtgc ttcttttgct gtttcgaatt ttataagatt tacatcaata
      181 cctctagaat ttgcaatttt tttcaaaata ggagcatatt tttctgtatg tggacactga
      241 ttactgtaat ataaaactat accgttctca tttaattttc ttttttttac acaattctta
      301 aaacatggaa taggagcatt atcagtaaag ggaaaatata atagctcata atttggttct
      361 gatgtatctg ctaaacaaaa gcccttatat tttagatatt tagcatcaga taaaaaaggc
      421 atttttttac tagacgaaag cacaactaat ccatatttac cttttgattt tgcatcatca
      481 acacactctt gtagcaatag attagaatat ccttgaccct taaattttcc agatatccaa
      541 aaacagttaa taaatatata attgtccgca gtaataggac accatgcatt ttctgctgga
      601 atatattcta taaaagcttt acctcttaca tcaaatcgtt taaaggctag acctttatca
      661 aaattatttt tcatccaagc ttttttagaa aaaacacaat tttctccttt tttttcagaa
      721 attgaacagc atatatgtct actgtcaata ttatttatat ttatatctat aatctgcata
      781 cttatctcct tttctaatta cataataatt tttaaatata ttctatatat ttttatctta
      841 atattttgaa tacaacaaca tcatgtcata tatattttta ttttacataa tattattttt
      901 agattatttg aattatcaaa ttgcatatat aattaaatac attctaggtc taaccacact
      961 atatataaac tatatattat cataatattc taaatttata aaacaaattg tattgaatta
     1021 aatttattta agatatataa ttataattat aattaaaata ataataaagg tgttgtttat
     1081 aatgttcaaa gaatacttaa taagagaatt aaaagaagat gatattaaaa atgcattaga
     1141 cttaatctgg aatgtatttg aagaatttga atctccagat tattgtgatg aaggaattac
     1201 gacatttcat caatttatag aatataattc tattattgaa aaaatcaaag aaaaaacact
     1261 cttcttttgg ggttgttttg actctaaaaa acttattgga gtcttagcta ttagaaattt
     1321 aaatcatata tgtatgttat ttgtagataa aaactatcat aaacatggtg ttgcaaaaaa
     1381 attaatgact acaggcatac aatactgtat ttgtaataaa tttaatttaa ctgaaataac
     1441 tgtaaattca tcaccttatg ctgttgaagt ttatcataaa ttaggatttg aagatattgc
     1501 tgttgagcaa attgttgacg gtatacgttt tacacctatg aaattgttaa tataataaat
     1561 taaataaaaa gcaaagtctc ttaaatattt tatagttact ttgcttttaa accaagattt
     1621 aaaataatac tatctttttt gttttttaat aatatacttt aaaagcctta ctaatatgaa
     1681 ctataattta ttttttgata tcctttttga ttaacatata aattaattca attattaaac
     1741 ataaactatg ttgtttctct ttatatctct tctttgtaaa caatattggg catttgaaaa
     1801 taaatattct taaaatatac tgttctcctg ttgattttcc tttggtacaa atgataaaat
     1861 tttaatttac atgctccgta atgaagtgtt taataaataa gttaaaataa tttatttacc
     1921 tccttaatca tattttttat gaatacctaa taacacagta tcaatagaat cggctaaacc
     1981 ttttatagct tcttctttac tcaaaagtga tttcccaagt tgcaaagtcg aataaccatg
     2041 aagtacactc attattaagt ttaatacttc acctgtattc tttgtattta gattacaaga
     2101 aagaataagt ttttctaata aggatttata ttcatcaaag atttctaatg tttcattatt
     2161 tccatgccaa gtagcccact gaattgtttc ataaacacca ggattagaaa ttataaaatt
     2221 aaagtatgct atacttatac actttatagc tgtatctcca aaatttccaa tagtcgcctt
     2281 tatcatttga ctattcatct ctctcattcc tttatgagct acttcaagta ataaatcatc
     2341 tagactttct atatgattgt acaaagatgg tgtacgtatt cctagctttt ctgcaataac
     2401 ttttaaagaa actttgttaa gcccatcttt attaactata tctgatgtag cttgtataac
     2461 tatttctcta ctaacacgca tgattttccc tctttcattt aaaattaatt actttattag
     2521 tttagctaat ctaattagtt tcgtcaatta ttgtaattta taaaattctt tatactctta
     2581 taaaatcttg acagtttaat tataatttaa tataatacta attgaattag ttttaatact
     2641 aatataatta gtattatatt aaattatata ttacaacatt attttatagt ttgattaatt
     2701 caacaatcac taatatatca aactgaaagg agatttttat ggaaaaaaaa tatttcgaaa
     2761 ttcatggagt tccttcaatc ctttggggaa atccttcaag taaattatat atttacatac
     2821 atggacaatg cggcaataaa gaggaggctg aagcttttgc taatattgca gttccacatg
     2881 agtatcaagt tttaagtgtt gatttgcctg aacatggaga acgtaaaaga gaaaaaaatt
     2941 catttaaccc atggcatgtt gttccagaat tagtatctat tatggaatat gcaaagtgcc
     3001 actggaatca aatttcgtta tatgcaaata gcattggagc atggtttagt atgttaagct
     3061 ttgaaaaaga gaacttaaga gagtgtattt ttgtatctcc aattctagat atgcaacagc
     3121 tcatttcaaa tatgatgctt tgggcaaatg tatcagaaga acagcttaaa catgaactaa
     3181 ttatcccaac ttcatttggg catactcttt catgggaata tctacaatat gtaaaacaac
     3241 atcctatcac tcaatggaat gtaccaacaa aaatattata tggtaaacat gatgaactta
     3301 ctgaaatcgg tgttgtagag aaatttgtag aaaagtttaa ttgcaacctt actattctgg
     3361 aaaatggcaa acattggttt aacacaccac aagaattaaa ttcattatat gaatggttaa
     3421 atactttagt taaataaaaa ataattatat tattaatatg attaaattat ttataaatgt
     3481 tatttaatta attttatttt agcattatta aaataactac ataaagaata aattagttat
     3541 tattaaagaa attaaaagta tatagagtat taataatttt ttaataatta gttattatag
     3601 aaaaaagagg gatataatat tgtctccctc tctttcttct gtaatattat ataattttta
     3661 tttatcactc tcaataaatt atagttatct acaaaattca atctttaata cattattaat
     3721 atattgtgat attaatatta cagtattaaa taaaatctaa taaaattaac tataaaatat
     3781 ctattatata aatatccact cttatgtttg aaatctgtca tatagtttta tccttcttat
     3841 cttttatatc aataactttt tttaataaaa ttgacaaaac aaagtaaaaa accttattga
     3901 taatgaaaca aatgatacta taagttctga tattatagta attgcatata aatagacatc
     3961 ataacaataa tataatattg tcactataaa attaaatgta aaaatctcca atctaactaa
     4021 atacacatat aggtattttt taagttttta ggtatttctt ctgaaaatga tttattgatt
     4081 gataccaatt tacataatct ttttaatata aacaatgata caatatatgg gacagcacat
     4141 atgtatatag ctacttatat tacatattac agttggcata ttcgtatttg taatgcttat
     4201 accaatttta aaatacacca tcagtactaa tggcattaaa ataatctata ataaagttaa
     4261 aattattcca attagtacaa tattatttaa tatttt
//
LOCUS       DAEPOZ010000267         4274 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.267, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000267 DAEPOZ010000000
VERSION     DAEPOZ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.267"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(166..1323)
                     /locus_tag="KR440_003321"
     CDS             complement(166..1323)
                     /locus_tag="KR440_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HBH0700325.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            1477..2046
                     /locus_tag="KR440_003322"
     CDS             1477..2046
                     /locus_tag="KR440_003322"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBH0700326.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNN"
     gene            complement(2228..2644)
                     /locus_tag="KR440_003323"
     CDS             complement(2228..2644)
                     /locus_tag="KR440_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700327.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(2870..3964)
                     /locus_tag="KR440_003324"
     CDS             complement(2870..3964)
                     /locus_tag="KR440_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700328.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
BASE COUNT         1470 a          754 c          451 g         1599 t
ORIGIN      
        1 tatatagtga tatttaatat tttaattatc actacttttt agaagaatac acactttatt
       61 ttaaagaccc acaaaagagt gcctcataaa ctaaaaagtt aattttgaga cactcctttt
      121 attaaggttc tttattttac aatataagtt tataaaaatt aaactttatt tatcaatctg
      181 ttctctaagc catttagtcg ctacatctaa ctgtttattt tcatcttttt cttcaatagt
      241 aggctttatg cctttttcat tgatataatc tccattaggt gtaaaatatt gagcagtagt
      301 taacttgtat cctgttccat ccttcaatct tacaactgat tgtaccaacc ctttaccaaa
      361 agttgtagtc cctacagaaa taccttcttt attatcaacg attgcagctg ttaatatttc
      421 agcagcagaa gcactttctc cattagttaa cactactatt ggaagtccca atttttcttt
      481 atcagatttc aagtactcag tatttccctt attatctttt gtatatacta tagttccttc
      541 accaattaac tcatcagcaa cttctttaca tatatctagc attccaccag gattatttct
      601 aaggtctatt actaatccct tgatattttt tgcttttaat tcttttaaag ctttatcaaa
      661 gtcagtatat gtacttgata taaattgggt aatttctata tatcctaaat cattatcaat
      721 tactttactt tctatactct tctcaattat ctggtctgtt tttactttaa agttcaattg
      781 tttatcttct cttaatatag tcagttcaac tgttttccct tttttaccct tcatcatacg
      841 aacagcttca tctgaatttt tatatgagac agattttcca ttgactttta ctaatttatc
      901 ccctgatttt acacctgctt tctctgctgg agaatctttc atgggaacta ctactaagcc
      961 accatctttt gatgccccta tatacatacc tacaccaaca taagaacctt cactatcatt
     1021 aattagattc tccatttcat ctttagtata ataaccagaa tacttatcat ttgttcctag
     1081 aaatagacct tttgctgctc catttaccaa atcagtatct gatacttttt tataaaaatc
     1141 ttgttttata atagattcta gtcctaacaa cttatcatat tttttatagt cttcatagag
     1201 ttctttcgat attacaactt tattccccaa tgtcaactga aatgctgatg taaccatagc
     1261 tgttataatt actaaaatca cccctaaaaa aatagctttc ttctttgaaa tcacaagata
     1321 catctccttt tacataaact attattatta tatgactagc tgttattttt attacattta
     1381 cccaaatata ttgctttatt acaataaatt atactaaaat ttatattaaa tacaattgct
     1441 gtacacatta taacatatta agggaggaat tattttatga aaaaagaatt tagaaaaaaa
     1501 gttattgaat caagaaaaaa taagagtgca aactttgtac ttagtaattc aaatttaata
     1561 actgaaaaat tacttcaaat ggaagatata agaaatgcaa ctaatataat gctttatcta
     1621 aattttaaca atgaagttca aacagataat ttaatcaaaa aacttttgtc tctaaagaaa
     1681 atagtatcaa gtcccatcac aataaaagaa aatcatacat taatccctac tcaaataaca
     1741 gatttaaaag agggattgag aattggagca tatggaataa gagaacctaa cgaaaaatca
     1801 cctacaattg atattaaggc cttagatgtt gtaattgtac cagctgtagc atatgatata
     1861 cattgctata gattaggata tggtggagga ttttatgata gatttttaga aaacttaaga
     1921 aaagatgcta taactattgg catagcattt gacttccaga tattcgattc tattccaaaa
     1981 gaaactcatg atgcacaatt agactacata gttactgaaa ctagaattat cactccaaat
     2041 aattagaaca ttaagtattt ccagacccca tttaatatag taataaatac tactgttaat
     2101 atcttacttt ataagcattt taagtcatgg cttttacaac aataagaacc atatacaatt
     2161 tacttacatt aataaaaacc atgactttat tgtacaatat gcagttattt aattataaaa
     2221 taccttctta tttcgtcgca tttaattcta ttctatctgg aagttcactc atatttgttt
     2281 caacaactat gtatggaaaa gaatcttcct tatctttata ctttatcttg actctcaaaa
     2341 catcctttcc ttcctcaacc acactttcta ttttgtccat ttctatacca tatttattat
     2401 ttcctctagt cactataatg tatattttgt tatcgatttt agccgctaat gctctttctt
     2461 catttgtata cttatccatc acatctaata tcttttctgg tattgaattt ctttgtaata
     2521 ctgtataatc tatgggttta gatttctcta caaacatact tggtatgaat ataaatcccc
     2581 ctataacgaa tacaagagct ataactccat aaattatacc tttaatatta aattttacat
     2641 tcaatcttat cactcctccc atatattcta aatatatgat ttataagctt atcttagact
     2701 ctaaattatt gattagtata tatttatcca taattataaa tatatgtaat gtaactaaaa
     2761 aacatgtttt tcctatttca taataaacga gctaaaaaat aaaaagagct atatgaaaac
     2821 attttcattg ttttcatata gctcttgagt ttacaatatt aaaaaatact tatttgtcac
     2881 tgattccttc ttgatatcca gatgtttttg taatctgtct ataaagaaca caaaccagta
     2941 ttatgacgcc catatatcca gtatatggat ataaaatacc aaccagctta tcaaatggta
     3001 ggaagccacc tataagacct aatatagctg ctattaaagt tattaattta aacttaggat
     3061 gtttatcatc tacaaatcta ttagttactg accataataa aggtacagct gttgtatata
     3121 tacctaatat aagaattatt gagaataaaa ctccaattac tggtgaaatc ttatctgcta
     3181 agaataatga tggtattgct tttgaatata agtttcctat atcagaaaga atagctaagt
     3241 gcatcattat agctgcagcc ataagtgcta ctccacctaa tataccacca caaatagctt
     3301 cttttttgtt agcagcactt gctcctaatc cagttaagaa tgccataaca acaattgtgt
     3361 tataagtagt atatattaca ccagatagat aggcatttgg aactggttta gctatcttta
     3421 cagtgctaag aacttgtgca gcttctccta tattacctat gtttttagct aaacttatac
     3481 caccaactaa aagtgtaaag attattatta taggccctat tccacctagt atctttgata
     3541 atttatcaag acctagtgaa acagttaata atgctactac tgccatacct actctaccta
     3601 caaatggatt taatccatag tattcagtta aagttgctcc tgctcctgaa atcattacta
     3661 cgaaaacacc aaataagaat aaaggtccaa accattcaaa gaatatccct aaatacttac
     3721 cacaataaac acgataaatt ttgataggtt ctttaagttg aagctcttta ccttttgaca
     3781 taactgaagc acccatccat gagaataata ccattgaaat tatcccacca ataatacctg
     3841 caaaaccata aaaagagaag aattgcatta tttcctgtcc tgttgcaaat ccagaaccga
     3901 ttactgttgc tacatacgca cctgcgaaac tgatgactgt tttaacattt actttttctg
     3961 acatatctca gttacctccc catgtgaaat tttttcttct gattaaataa ttcgataaac
     4021 tacctacaat ttcctttaac ataaaataca tgataactgt tttatagctt tttagtataa
     4081 taacttaaaa cttataatct gattttatac cctactcctc ctctcataat ttttttagat
     4141 tcttttatct aagtagtcat tatatataaa attagtttct ctatagatta tatattagtc
     4201 atatttttct gaatagtcaa tatatatttg aatattttct ctatttttgt aaaaatttga
     4261 atattttatt acat
//
LOCUS       DAEPOZ010000268         4234 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.268, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000268 DAEPOZ010000000
VERSION     DAEPOZ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.268"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(102..593)
                     /locus_tag="KR440_003325"
     CDS             complement(102..593)
                     /locus_tag="KR440_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HBH0700329.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDENIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASDDGLAVIF
                     FIM"
     gene            complement(1687..3822)
                     /gene="rnr"
                     /locus_tag="KR440_003326"
     CDS             complement(1687..3822)
                     /gene="rnr"
                     /locus_tag="KR440_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBH0700330.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILAKILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
BASE COUNT         1337 a          706 c          374 g         1817 t
ORIGIN      
        1 ttttaaaagg ctaatataat atgtattttc ttctatacaa tcaaaaggct tcatatctaa
       61 gatatgttat gacactaata ttcacaattt tttagtaatt atcacattat aaaaaatatt
      121 acagctagtc catcatcaga tgcttcttgt ataaactttt taagctcaat tagatttgcc
      181 catacatatt caaaaaaatc atcttcactc tcatcattca taacaggata aatattattt
      241 tctaacatct cattaatatt aaatcttttt ctaaatgctt gttcttccaa actgttcatt
      301 gcttttgata gtttttttac atcatttgct gtaatcaaca tgacaggaaa ttctccatca
      361 atttccataa gtatgtttcc acttagtact aatttgctaa atatattctc atcatctcca
      421 ccaaaaggac aacctgtcaa agtaaagtga atggcatgcc atgctttatc aatattgata
      481 gtactatttt cgttggcatc ttcaaaaaca aaatcttgta aattttcttc tgcttgctgt
      541 aatttaaaaa cattctcttc acttatttta gtataacatc ctatcatccc cataattaca
      601 ttcctcctaa gtagcgtttt tttaattgta acttataatt aaacttagtt acaataaaat
      661 atattgagaa aagtattatt tcatcttaaa tttaaatacc tatattatca actaaaattc
      721 tgtgtttata gcgtaagtat tattattttt tcctaaaaat taaatgaatc agaagttcta
      781 taggcaatat tgactataaa taagaactat tacttttaaa aaaaatttaa gaaattatac
      841 tgtagaatca tgctctactt attagcttca aataaaaaat aaatcatgtt ctcaaattaa
      901 ataataaata acaataaatt taaattttaa actataagag tttctgaagt gttaataagc
      961 ataccaataa ttatatgtgt aagaatttga tttaatagat atgaataatt tttacattaa
     1021 ttataaatat attaacctat tattataaat tctataaaaa gaaaagttat cttaaataaa
     1081 aaataagtgt atcttaaatt ttaaatgata cacttatttt tatattgaaa ttatattttt
     1141 taatatctat tcttaattag gtatttttaa attatctgtt tacattaaat atctcttaat
     1201 tacttatctt tcagctagct tcttctatta aatgtttctc aattacttat ttttcaatta
     1261 gccacttcta ttaaacattt cttaattact tatttttcaa ttagccactt ctattaaaca
     1321 tttcttaatt acttattttt caattagcca cttctattaa gcatttctta attacttatt
     1381 tttcaattag tcacttccat taaacatttc ttaattattt atttttcagt tggctttttc
     1441 tattaaatgt ttcttaatta cttatctttc agttagctct ttctattaaa tatttctcaa
     1501 ttacttattt ttcaattagc tactttcatt aaatatttct taattattta tctttaagtt
     1561 aactatttcc attagatatt tcttaattac ctatttttat catttatttt atagagtatt
     1621 gtgcactata tatttataca tatcataaat actacaactt gttatctctt attttagctg
     1681 ttttatctac tcctctattt tttcaactat ctcaaagtca atttctttaa aatctacatt
     1741 aacactttca acctttatct taacaaggtc tcctatctta tatgacttct tagttctttc
     1801 tcctaaaaca ctataagaac tctcatcata aatatagtaa tcatcattca tatttgcaag
     1861 tcttatcaat ccttctatag tattatctaa ctcaacaaat attccaaatg aagttacgct
     1921 agatacaaca ccctcaaatt cttgaccaac tttatccttc atataaagag ccttatagaa
     1981 atcatgtaca tctctttcag ctaactctgc ctttctttct ttctcggatg attgaacaga
     2041 tgcataatcc actatagtag taagctgttc ttgtcttttt ccacttattt tattatttaa
     2101 agattctttt attattctat gaatttgaag gtcaggatat cttcttattg gtgaagtaaa
     2161 gtgacagtag aatttagctg ctaatccaaa atgtccacta cattctggtg agtacttagc
     2221 ttgttttaaa gaacgaagca ttatagtact gatagctcct tcttcttttt tgccctttat
     2281 tttctctatt ataccttgta atgcttttgg atgtacttcc tctttatcac cttttattat
     2341 atatccaaat gtagatataa acttactcaa ttcttccatt ttttccaaag atggtgtctc
     2401 atgtattcta tacacaaacg gtattcccat ccagtaaaaa tgttcggcaa ttgtctcatt
     2461 acttataagc ataaattctt ctattatctt attagatatt cttctttcat atggctttat
     2521 atctacaact tcgccttttc catttagaat tatctttgcc tctgggaaat tgaagtctat
     2581 agctcctctt ttttctcttc ttgacataag aatcttagct aaaatttcag catttttaaa
     2641 atcttcaaca acatgttcaa aagtcttttt aagtttttca tcatcctttt ctaatatatc
     2701 agatacttct gtataagtca ttctagcttt tgaatttatt acagattcac atatttcatg
     2761 tttaaccaca tttcctttat ggtctatttc cataaatata gaaagagtta acttatcttc
     2821 aaatggattt aagctacata ctccatttga aagagttttt ggcaacattg gaattacttt
     2881 atctactaaa taaactgatg tagctctttt taaggcttcc ttatcaagtt tatttttctc
     2941 tcttacataa tgtgtaacat cagctatgtg aactcctagt ttgaagtttc cattaggtaa
     3001 tacttctatt gatatggcat catctaagtc ttttgcgtca tctccatcta tagtaaatat
     3061 gtttaaatct cttaaatctc ttcttctttt tatctcttct tgaggaatct caacagctac
     3121 agcttctgct tcttgcaata ctttctttgg aaattcttct ggaagaccat gttctcttat
     3181 tatagaatct atttctacgc ctctttcacc tttttgacct agtatctcta taattttgcc
     3241 ttctggtttt ctatcctctt gtggccatac tgtaatttca catactacct tatcattatc
     3301 tactgctcca ctaaaatgtt ttttaggtat atatatatct ttagtaaatt tcttgttgtc
     3361 tggtgttaca aatccaaaag ttttacttga ttggaaagta cctactattc tagttatttc
     3421 tctctttatt acttttacta ttcttccttc tgctctcttt tcttcagttg caggtactac
     3481 tatttctgcc ataactctat cgttatgcat agcaccattt atattgtcac ttgagataaa
     3541 taaatcctgt gtatattcaa catcagattc tacaaatcca aaaccctttt tgtgtgatac
     3601 aaattttcca acaaagtatc ccatttgatt gggagatact atttttcctt ttttagtaaa
     3661 gcagatataa ccatcttctt ctaattcatt taaaaaatta taaaacattg gcatttctga
     3721 tgaatgtata tcaaatattt ctgctaattc ttcttttttt agaggattat atgcactttc
     3781 atttattaat cctagcaaac tttctttaag tcctggtatc atttttccct cctgttttac
     3841 ttttcattat attattattt atattgtctt ttatatttct tcagataaaa cattttttac
     3901 ttatcacata attttctata ttttcttatt attattacaa gttaaaatta cttatttata
     3961 tttttgattt aatgtaagct ttgtcatact aacttcaaac aaattatgat gttttataaa
     4021 ttatttgtaa atatcattat aattacttaa gttattcttt atcataattg cttaaattac
     4081 tctttatctc ataattatac accatcaaat aggattggta aaatattcat ctcatataag
     4141 tattactata taataattta ttctattagt atactcaaat catgtaaaat ttatataact
     4201 caattaattt tttaaataca actttataat ataa
//
LOCUS       DAEPOZ010000269         4217 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.269, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000269 DAEPOZ010000000
VERSION     DAEPOZ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.269"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            242..2341
                     /gene="nrdE"
                     /locus_tag="KR440_003327"
     CDS             242..2341
                     /gene="nrdE"
                     /locus_tag="KR440_003327"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBH0700331.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYLKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGDKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDEWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            2354..3325
                     /gene="nrdF"
                     /locus_tag="KR440_003328"
     CDS             2354..3325
                     /gene="nrdF"
                     /locus_tag="KR440_003328"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBH0700332.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKKMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            3401..4027
                     /locus_tag="KR440_003329"
     CDS             3401..4027
                     /locus_tag="KR440_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBH0700333.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIIEIPEQKFIMLKGSGDPNSKEFI
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
BASE COUNT         1694 a          449 c          776 g         1298 t
ORIGIN      
        1 aaaataatgt caattataaa gtaataaaag tttagatata aaaaaatttg tgtatatact
       61 atatgtagta tataatagat aaagcactga ctatatgtag tgcttttgtt tttgaaaaat
      121 acttattgca tttctataat tatagattaa ttttgggatt tcaactgata agtaccaaaa
      181 gaaataataa gcaaattaag tgaaattatg gcacaagatt atgttaaaag gacggtaaat
      241 aatgaattgg atagagctta acaatcaagt gataattaaa aatgaacaag gtaagtacca
      301 attggaaaag gataaggaag ccttaagtag ttacatagaa gaatttatac aaccaaagtt
      361 gagaaaattt aataacctag aagaaagatt gaagtattta atagatgaag gttattattt
      421 aaaagaagta ataaatcagt atagcataga tgttattaaa aatatatatg atatgattga
      481 taaacataag tttgaatttc aatcatatat gagtgcaaat aagttttatc aaaattatgc
      541 attaaagagc aatgatggaa aggaaatatt agaaacatat aatgataaag tgttaattgt
      601 agctttgaca ctaggaaatg gcgataaaga tttagcttta aacttggcag ataaaatagt
      661 aaaacaagaa tttcaaccag ctacacctac ttttttaaat gctggaagaa agagggctgg
      721 agaaatggta tcatgttttt tactatctgt agaagatagt acagaaggta tatcatatgc
      781 tatatcttca tcaaatcact tatcaaagat tggtggagga gttgcactaa atttatctaa
      841 acttagagca tctggcgaat ctatcaaaga tatagaaggt gcagctggag gtgttgttgg
      901 agtagctaaa atgttagaac aatcatttag ttactttaat caaatgggag ctagacaagg
      961 ttctggtgcg gtgtacctta ctgtattcca tcctgatttt gaattgttga tggatactaa
     1021 aaagataaat gcagatgaaa aaataagact tgctacatta tcattaggag ctataatacc
     1081 agataaattt atggagttgg cagaaaaaaa tgaagtagca tatgcatttt atcctcatac
     1141 tgtgtataag aaatatggag tttctcttga tgagatagaa atggatgagt ggtatgataa
     1201 attggttaat gatagtgata taagaaaaaa agaaataaat cctaggcaga tgttgacaaa
     1261 aatagcacaa atgcaacaag aaagtggata tccatatgta gtgtacatag atacagctaa
     1321 tagagaacat acattaaaag atgttggaat tattaaaatg tctaacttat gttgtgagat
     1381 atttcaatat caaacacctt cagaaataga ggggtatgga ggaaaaaatg aatggggaca
     1441 agatataagt tgtaacctag gttctttaaa catagccaat gtaatggata ataaaactat
     1501 agaaagtaca gttgaaacag caataagagc actgtcattt gtagcagata gcacagatat
     1561 aaaaccagtg ccaacggtaa gcaatagtaa cagcaagtct cattctatag gattaggagc
     1621 tatgaattta catggatact tagtaagaga aaatatttta tatacttcag atgatgctat
     1681 agatttttct aatgtatttt ttgcaatggt tagatactat tcaattaaag cttctatgaa
     1741 aatagccata gaaagaaatc aaacttttga aggatttgat aaatcagaat atgttaaggg
     1801 aagaaatagc aaggttttat caaaatacta tgaacaatct tatctaccta agtcagaaaa
     1861 ggtaagagca ttatttgaag ggatttatat accaacaaaa gaagattgga caaaattatt
     1921 ggatgaagta aaagaaaaag ggatatataa tgcatattta atggcagtag caccaactca
     1981 aagcattagt tatgttcaaa atgctacatc aagtataatg ccaattactg agccagtaga
     2041 agtaagaaca tatggagact ctactacaat atatccaatg ccatttttaa caaatgataa
     2101 tatgttgtat tatcaatcag catacaggat ggatatgaga aaggtcatag atttagtggc
     2161 aacagttcaa aaccatgttg accaaggtat atcaacaaca ctgtttgtta cagatgaaaa
     2221 gacaactaga gatatagcta gacattacat atatgcctat aaaaaaggtt tgaaaagttt
     2281 atattacaca agaacaaaga tgacaagaga tactcatgag tgtctagtgt gttcagtata
     2341 gggagagaaa tatatgacat ttaatttaca taaaatacat aatgcagtta actggaatag
     2401 agaagaagat ggatttacac aagctttttg ggaacaaaat gtaaaacaat tttggcttcc
     2461 tgaagaaata tctgtttcta aagacataaa ggtatggaat gaactaagta ataaagaaaa
     2521 ggaattatat aaaaaagtat tgggtggttt aacattactg gatacaaaac aggggaataa
     2581 tggaatacca tctatgatga gtttaacaga aaacctacaa agaaaagctg tcttgtcttt
     2641 tatggggact atggaagaaa tacatgcaaa aagttattct tctatattta tgactctact
     2701 ttctaattta gaaatagatg aattatttga atggatagaa acagaaccaa cattacaaag
     2761 aaaggctgat ttagttttag ctcaatacga aaatactaca aatcaagagg gattatacct
     2821 atcaatggtt acaagtgtat ttttagagag tttcttgttt tattcaggat tcttttatcc
     2881 attgtatcta tctggtcaag gaaagatggt agctagtgga gaaataatat cgcttattct
     2941 tagagatgag tctttacatg gtaagtatat aggattgcta gcacaagaaa tatacgattc
     3001 atttgataaa atggataaga agatgttaga agagaagatg tattctatac tttatagcct
     3061 tatggagaat gagatagagt atacaaatgt tatatacaga gaaagtgggc ttgaaaaaga
     3121 ggttgtgaac tttttaaaat acaatgcgaa tagagcttta gaaaatctag ggtttgagaa
     3181 gctatatact gtagatgcta taaatccaat tgtattaaat ggtcttagca ctgaaaccaa
     3241 aacacatgat ttcttttcaa ctaagggtaa tggataccaa aaaggagtgt atgaggaatt
     3301 agaggatgaa gattttataa tataatttat aattatgaca cactgttgtc acttttagcg
     3361 tgataatatc aatgtataaa atatatatgg aggtattttt atgaaatatg aatggagaaa
     3421 aagagataaa gagttatatt tacctaaaaa gaatcctgta ataattgaaa taccagaaca
     3481 gaaatttatc atgttaaaag ggagtggaga cccaaattct aaagaattta ttgaagcaat
     3541 aggagtactt tattctttgg catatgcaat aaaaaccatg cctaaaaaag gaattgtacc
     3601 agaagggtat tttgactata cggtttttcc tttagaaggt atatgggata agggagaaaa
     3661 aagtaaaggc ttagatgtac ttattaaaga agatttaatt tacactataa tgatacgtca
     3721 gccagatttt gttacacaag acctgttgga taaagctact gatatagtaa agaagaagaa
     3781 acctcataat cttcttgaaa aagttaggtt tgggtctatt aatgaagggc tatgtgttca
     3841 aatgcttcat ataggttcat atgataatga accagaaagt tttgacatta tgaacgaatt
     3901 ttgcaaaaaa aataaactta taagaaaatc ttacacacat cgtgaaattt atataagtga
     3961 tgctagaaaa gtatcgcctg aatcattgaa gacggtttta cgttttgaag tagaaaggga
     4021 agtataaata ttttgaataa tttataagag ttataaaaag tgaagtctgt tgagacttca
     4081 ttttttgatg caattttaaa attaaatttg atgtttcata atattttaaa aactttctac
     4141 ttttattagt gtgaataact gttgctatta aataataata ctaattatat aagtattatt
     4201 atataatatt tatagat
//
LOCUS       DAEPOZ010000270         4211 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.270, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000270 DAEPOZ010000000
VERSION     DAEPOZ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.270"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            425..1681
                     /locus_tag="KR440_003330"
     CDS             425..1681
                     /locus_tag="KR440_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBH0700334.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFLLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNASSIFLV
                     TGIAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASAVSKTLGLEKFS
                     FALSSKVVFDLPIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
     gene            1744..2937
                     /locus_tag="KR440_003331"
     CDS             1744..2937
                     /locus_tag="KR440_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBH0700335.1"
                     /translation="MLTSLALIFLLGMASGGIFEKIKLPSLLGMLLTGIILGSYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIVGAVIGICLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMNIGIFLQIKYRVVARRLSVKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGIASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     EGKSK"
     gene            3144..3524
                     /locus_tag="KR440_003332"
     CDS             3144..3524
                     /locus_tag="KR440_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH0700336.1"
                     /translation="MYLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKILSKLSKKRFLYVQETGKCMYYTVAIKEKTYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYI"
BASE COUNT         1485 a          430 c          743 g         1553 t
ORIGIN      
        1 attttaattg ataggttaca ttatttataa cattaaaaat acaatttaca taaaatagac
       61 atatgaaaaa taaaaaatta tataataatt atgggaatga ggttctccct tggatgataa
      121 tatcctaaac tgccaatgag ctgatgactt ctatgatatc atagatgcca ccagcttttt
      181 ttgttgttta aatcgataaa atggaggtga attacttact aaatttttat tttttaaatt
      241 tgaagaaata tttaattaat agaaaaaaca gtagattata tgttaattat tttattaaga
      301 atgggttaag agtataccta tatcctatca atttaaaaaa caattatgat agtcattaca
      361 ttgactacag ttgatgcaat aaaactttca aaaaatctta tgatgcaaat agaaaggaat
      421 taaaatgata gaaaaattta aaactttaat atggattaaa atgataataa gaattaaaag
      481 cgtttataaa acatatggtg gactattttt gttaggactc ataggtatac cagttggtgc
      541 aattataggt cttatagaca ccatatttgg aacggttctt ctaaaagtta cagatattcg
      601 agaaacatat ccaatgtatc tgataccatt tttagcagta gtaggagttg ttattgctta
      661 ttgctatttt aaatttggag gaaaaagtag taaaggaatg aatctaatat ttgaggtagg
      721 gcatggagaa gaagaaatta ttcctttaag attggttcca tttataatat caggtacatg
      781 gttaacccat ttatttggtg gtagtgcagg acgagaagga gttgctgttc agattggtgc
      841 aactttttca cattgggtag gtaaaaggtt gcctataaaa aatgcttcta gtatttttct
      901 agtaactggt atagcagctg gttttgcagg attatttgag acaccaatag cagcaatact
      961 atttgctatg gaggttttgg tagctggttc actggagtat caatcgcttt ttcctgcgtt
     1021 tactgcttct tttacagcaa gtgcagtatc aaaaacttta gggttagaga aattttcttt
     1081 tgcactttct tctaaagttg tatttgactt accaatattt tggaagctta ttgttttagg
     1141 aattattttt ggaatggttg gtggagcatt tgcatggtgc ctgaaattgt caaaaagaaa
     1201 aataggtaat agattaaaaa atcctatgat aaggattgct attattggag tatgtcttag
     1261 tgtattattc cttttatttt ataaaggaag atattcaggt ttagggacaa atttaattca
     1321 aaatagtttt tatggtggag aaatttattc atttgactgg ctgttaaagt ttatacttac
     1381 aatactgact ttgtcagcag gttttcaagg aggagaggtt acacctttgt tttccattgg
     1441 agctagtctt ggtgtattgt tagcaggatt ttttaatttg ccaattgaat tagttgcagc
     1501 gcttggctat gctagcgtct ttggcagtgc tacaaatact ttttttgctc ctgtatttat
     1561 aggggcagaa gtttttggat acagctattt accttatttc tttgtagtat gcgctatttc
     1621 atatatattt aatatggata aatctattta ttcactacaa aaaatttcaa cgaaacaata
     1681 aataaaacaa cataatgtat gattataaga gaaagaatat tagagaaagg atagggtagg
     1741 aatatgttga caagtttagc tttgattttt ttattaggaa tggcctcggg aggtattttt
     1801 gagaagataa aattacctag cttattggga atgttactta caggaatcat attgggttca
     1861 tatgttttaa atttgattga taattcaata ctagatattt cttctgacct tagaaagatt
     1921 gcattgatta ttatattaac aagggctgga ttatctttag atataaacga tttgaagaaa
     1981 gtgggaagac ctgctgtact tatgtgcttt attccagcta cttttgaaat tatcggaatg
     2041 atagttttag caccaaaatt actaggcgtt tctatattgg aagcagcagt catgggtgca
     2101 gtagtaggag cagtttctcc cgcaattatt gtacctaaaa tgcttaaatt aatggaagaa
     2161 ggttatggta cagaaaaaag tattccacag atgcttttag caggaacttc tatagacgat
     2221 attttcgtaa ttgtgatgtt tacagtattc acaggattgg cacaaggaaa tagtatatca
     2281 gctattagtt ttttacaaat tccagtatca attatattag gtgtaatagt tggagcagtg
     2341 attggaatct gtttagcagt attttttaag aaggttcata tgagagattc tgctaaaggg
     2401 gtgttgcttt taagtatctc atttttaatg ataagtttgg agactgcttt agagggtatt
     2461 gtgccatttt ctggattatt agcagttatg aatattggga ttttccttca gataaaatat
     2521 cgtgtagttg caagaagatt gtctgtaaag tattcaaaac tatgggttgg tgctgaaatt
     2581 ctcttgtttg ttttggtagg ggcaacagta gatataagtt atgcatttaa agcaggaatt
     2641 ggagctgtaa ttttaatatt tggagttctt ttattccgta tggtaggagt attcttctgc
     2701 cttattaaga caaatttgac aataaaagaa agattgttct gtatgattgg ctatattcca
     2761 aaggctacag tacaggcagc aataggaggt gtaccacttg caatggggat agcatctgga
     2821 caacttattt tgacattagc ggttctcgct atacttataa ctgcacctct tggagctttt
     2881 ggaattgatg taacttataa aaaattatta acttctgtag aaggtaagag taagtaagaa
     2941 taaaaataaa gattatttta agtgataaat tatattttgg aaaaaactat attattgagt
     3001 acaatgttag aaaatcattt gagtgattac agaaggtaga agtttatatc ggattttaag
     3061 gcatgtgatg attattagaa ttgattttgt gaaattgtta ttgtaatatt taattatata
     3121 gataaagaag cggagtgata actatgtatt taaaaaaatt atcaaaatta gagttagtta
     3181 ttatgaaatt tatttggaat ttagatataa aaaccaattc atatgagatt attgattata
     3241 tgaaagaaga gcataattta ccagaaaaag tggctttaaa aattttatca aaattatcaa
     3301 agaaaagatt cttatatgtt caagaaacag gcaagtgtat gtattataca gttgcaataa
     3361 aagaaaagac atatttagag tttatatcaa gaaatgtgca aaatcttttg aagaataatt
     3421 ttataaggaa tttattagta tcatttcacg aagaggagct aacagaggaa aagataaagt
     3481 cgttagaaaa ctgggtagta aattgggaag aagcttatat ataaatgtta ttatttttat
     3541 tacaatttag ataattttgg ttttgtaagc tctgcaacta tttttagatg gttgcagagc
     3601 ttatttttat tttttattcc cttaagcaat acttaaaagt aaacgcattt atatttacta
     3661 acttcaatca cttctaatac ttagattact ttttaaaaat attttgatat gtcatctcta
     3721 tagtaatcta gaacttgttt ctattcttta tataaagttt ttatgaatga tatatataat
     3781 attcgttcat ttgattaaaa ataacaaaat attaaataat tctactctga tagtttgtta
     3841 aaaaaataat aaaaaatatt aataaacaaa aaaaaaaatt atcttaagag aggagaatgt
     3901 tctaaaatat aaaaaggttt ctagatttca taaaagatac tattttagtc ttgaaaatat
     3961 ttagtttgaa aagattttaa tttaatgatt gattaagtta aaaatgtgca tgtaataaat
     4021 tttattttat ttattgacta aattataaag tttacataat tatttaataa ttatgtaatt
     4081 gttacttaaa aattgatcta ttttaaaatc tagttataac ttcgaaaaag actgaaaatt
     4141 aagaaaaaag aaatataagt ataaaaatat atcatataga ttttttttat tttactttaa
     4201 taaaatgatt t
//
LOCUS       DAEPOZ010000271         4169 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.271, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000271 DAEPOZ010000000
VERSION     DAEPOZ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.271"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     misc_binding    68..330
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            406..3072
                     /locus_tag="KR440_003333"
     CDS             406..3072
                     /locus_tag="KR440_003333"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBH0700337.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKAHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKVKKEKFEEMIKSVEERLTNLESKIK"
     gene            3497..>4169
                     /gene="rgaR"
                     /locus_tag="KR440_003334"
     CDS             3497..>4169
                     /gene="rgaR"
                     /locus_tag="KR440_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HBH0700338.1"
                     /translation="MFRVVICDDEKIQRSILKEFTKKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLK"
BASE COUNT         1678 a          429 c          779 g         1283 t
ORIGIN      
        1 attcagaaaa aataaaataa aaataattga caaagagaat aatatttgta tataataata
       61 tgtaatatta aagactgaga agaagaggag taagtaaata ctactaaaag agaggaaaat
      121 tcactggctg taagattttc taagtataag atttactgaa tgtagctttg gagtttctaa
      181 actgaactat aagtaagttt agacggttag aaccgttaaa ttctttgaga gtcaaagttg
      241 ataaaataac attaactaat aatttaatta tatgttaatg tattttaagc tttggaatta
      301 aggtggtaac gcgagctttt cgtccttttt aaagaggatg aagagctctt ttttatttct
      361 aaagatattt aaaaaattta tttataaaat acaggaggca ggacaatgga aaatacgaat
      421 ttatcaaaaa cttataaccc aaaagatttt gaggctaggc tatataaaaa atggatggat
      481 gaagggtatt ttaaatctaa accaaatcca gataaaaaac catttacaat aatgatgcca
      541 ccaccaaata taacagggca attgcatatg ggacacgctc ttgaccatac acttcaagat
      601 atattaatca gatggaaacg tatggatgga tatgaagctt tttggcttcc aggaactgac
      661 catgcttcta tagcaacaga agttaaagtt gttgaaagaa taaaaaaaca agaaggcaag
      721 acaaagtatg aaataggtag agaagaattt ttaaagagag cctgggaatg gaaagatgaa
      781 tttggaggaa aaatatctaa tcagcttaaa caactaggtg attcttgtga ttgggataaa
      841 gaaagattta ctatggatga agggtgtaat gaagcagtta tagaattttt tgtaagccta
      901 tatgaaaaag gtcacatata cagaggaaat agaataataa actggtgccc agattgcaaa
      961 actactttat cagatgcaga agttgaacat gaagaacacg acggaaactt ttatcatata
     1021 aagtacccac taaaagatag tgaagacttt ttagaaatag ctacaactag acctgagaca
     1081 atgataggag atactggtat agcagtaaat ccagaagatg atagatataa acatttaata
     1141 ggaaaaacag caatacttcc attagtagga agagaacttc caatagttgc agatagttat
     1201 gttgatttag agtttggaac tggtgcagtt aagatgactc cagcacatga ccctaatgat
     1261 tttgaagtag gattaagaca taatcttgag cagttaaata ctatgaatga agatggaact
     1321 atgaatgaag tttgtggaaa atatgaaggt atggatagat ttgagtgtag aaaagctatc
     1381 gtagcagact taaaagaaca aggttaccta attaagataa aagcgcataa tcataatgta
     1441 ggaacttgtt atagatgcca tacagtagtt gagcctagat tatcagagca atggtttgtt
     1501 aagatggaag aacttgcaaa accagctata gatattctta aaaaaggaga attagaattt
     1561 gttccagata aatttgataa aacttattta caatggctag aaaatataag agattggtgt
     1621 atatcaagac aattatggtg gggtcatcaa ataccagctt attattgtca agagtgtgga
     1681 gaaatagtag ttgctagaaa aatgccagat aagtgtccta aatgtggaag cactcatttt
     1741 aaacaagatg aagatgcctt agatacttgg ttcagttctg cattatggcc tttctctact
     1801 ctaggatggc caaataagac tgaagcttta gattattatt atccaacaag tgtacttgtt
     1861 acaggatacg atataatatt cttctgggta gttagaatgg catttgcagg tatgttctgt
     1921 atgaatgaaa agccatttga ccatgttttg gttcatggat tagtaagaga ttctcaaggt
     1981 agaaaaatga gtaaatcttt aggaaatggt atagacccat tggagataat cgagcaatat
     2041 ggagctgatg ccctaagatt tacattaaca actggaaatt ccccaggaaa tgatatgaga
     2101 ttctatatgg aaagagtaga atttgctaga aactttgcaa ataagttgtg gaatgcatca
     2161 agatttgtat ttatgaatat tgatgaagat ataattaaaa acatgactag agaaagtgtt
     2221 aaagaggact taactctagc tgataaatgg ataatatcaa gagcaaataa tattgtaaaa
     2281 gaagcaacta ataacatgga taagtttgat ttaggaatag cacttcaaaa gatatacgat
     2341 tttacttggt cagaatactg tgactggtat attgaaatgg ttaaacctag attatatggg
     2401 gaagatgcaa atgctaaatc tgcagcatta tatacattaa cttatgttct tgaaaaaata
     2461 ttaaaattat tacatccata catgccattt ataacagagg aaatatatac tcatcttcct
     2521 actgttgaag gatgtataat agtatctgaa tggccaaaat ataatgaaga agataacatg
     2581 gctgaagaag aagatatgat gaacttattg atggaaggta taagaagtat aagaaatgtg
     2641 agagcagaaa tgaatgtacc accttctaag aaagctaaat tgataataat cccaagtgaa
     2701 gaaaaaatag aagctattga gcttggtaaa gactatttta taactctagc atctgcttca
     2761 aatgtagaga tagcaaaaga taaatctaat gttccagaag atgcagttgg agtagttatt
     2821 gatggtgttg aaatatttat acctcttaat gaattagttg attttgaaaa agaaattgag
     2881 agattgtcta aagaaaagaa aaagttagaa ggcgaaataa agagagttaa tggaaagctt
     2941 gcaaatcaag gtttcttagc aaaagctcct gaaagtttaa ttgaagaaga aaaagtaaaa
     3001 aaagaaaaat ttgaagagat gattaaatct gttgaggaaa gattaacaaa tttagaatct
     3061 aaaataaaat aaatataaga attagtaaaa acatacaaaa agtatatatt ctatactttt
     3121 tgtatgtttt tctttatata tatacaaata tgatgttata attctttaca gatgtgttat
     3181 atttttattt tatgttgtga atatatgaat tttatgtctt aaaatttata agcaataaaa
     3241 atttgatata ttatgatgtt taatataggg taataaatac atattatatt tattttacta
     3301 tattttttta cataatatat ctagaaaagt ataggaattt atcgtaattt aagtttaaat
     3361 attaaagaga atttctataa gtagtttaat cgacaactta aatttataaa gaaatttaaa
     3421 tttgaaagct agtgagtaca gtttggaaat tgaaataaag taaactaaat gatattttag
     3481 atttggaggt gatattatgt tcagggtagt tatatgtgat gacgaaaaaa tacaaagaag
     3541 catactaaaa gagtttacaa aaaaactttt gtctgaaaga tgtctaaatt atcaaatact
     3601 ggaattttcc tgtggagaag atttaatatc caaataccca gaaaaaattg atattatatt
     3661 tttagatatc caaatgaaag atatcaatgg aattgaggtg gctaaaaaaa taagaaagtt
     3721 tgatgaaaaa gtagaaatta tatttacaac agctttttca gagtatgccc cccaaggtta
     3781 tgaggttcgt gcttatagat atttagtaaa gcctatagag tatagtaact ttgcaatggg
     3841 tgtaaatctt tgtattgata accttcaaag aaagaaggag cgttatatag ttttaaatag
     3901 taaaaaagga tttaatagaa tactgataga ttctatttta tatgttgaaa ctgtaaagag
     3961 agatttagtt gtacatacaa taggtagaga ttataaagct aatatgagca tgaaacaagc
     4021 agaaaaatta ctgtgtgaaa atggcttttt tagatgtcat acaagtttta ttgttaattt
     4081 aagaagaata gaagatctta gggataatat gattactatt aatgaacaat ttatacctat
     4141 aagtaagtat agacttaaag atttaaaag
//
LOCUS       DAEPOZ010000272         4158 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.272, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000272 DAEPOZ010000000
VERSION     DAEPOZ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.272"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(214..723)
                     /locus_tag="KR440_003335"
     CDS             complement(214..723)
                     /locus_tag="KR440_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBH0700339.1"
                     /translation="MSQQNTGNAIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(725..1372)
                     /locus_tag="KR440_003336"
     CDS             complement(725..1372)
                     /locus_tag="KR440_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700340.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            complement(1386..2558)
                     /locus_tag="KR440_003337"
     CDS             complement(1386..2558)
                     /locus_tag="KR440_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBH0700341.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNIESYTHCKESVNKLAE
                     RLKLEFEKEGLDCELVDVGENGNTLIGTLGSNIDKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYVIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGALCAFNMETGLVDNSLCIGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSRENGFGELNAKRLGG
                     SSDASYLTIANVPTICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            2826..>4158
                     /locus_tag="KR440_003338"
     CDS             2826..>4158
                     /locus_tag="KR440_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700342.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDERDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDCIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEILNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILADYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKV"
BASE COUNT         1455 a          732 c          468 g         1503 t
ORIGIN      
        1 tttcattgtt gaaattatga aaatatactt gatttaatac caaaagagta tctcatgaac
       61 taaaaagttc attttgagat actctcttct tttttaatta atatacatat taattactat
      121 attagtttat tactatatat caattactat tcattaatta ctattcatca attcttatat
      181 atcatttata attaggttat aattttatgt tatttacatt aatcccataa cagtgagtaa
      241 aactcttact atcaacatcc ccactattgc atcaaatata ggaactgcca acagtaacat
      301 tctatactta gtactggcat ttgctacaag cactattctg gcataatgac caactagagt
      361 acccattaac atacatggca taattaatat ggttgcttgt ggtgcagtga ttagaccttg
      421 tgcaaataag tttgcagctg tagctgctcc tgctgcctta gcaaagaatg ctgctatcaa
      481 aactacaaca gattccccag gtagtccaaa aatacccatt actggaccaa aaatatttcc
      541 taaaatatca actaaaccag ttaattgtaa aaactgcact attacataac ccattatcat
      601 ggctggtaat atctgttcaa cacctatgta aaaacctttt tttgctcccc ccataaatat
      661 ctcaacagca gttttcttag gtttactatt tacaattgca tttccagtat tttgttgact
      721 catactacac agcctcctta gccttattct ttttatttct tacacctatg attaatctaa
      781 ctagattagc accaagtatt ttacagaata tctctataag gattattgga cctaatgcca
      841 ataatgctat tggtaaaagt ggagcttgag tatttattgt gttcagtata acagctgaac
      901 cagcatattg atatgctaca aatatagttc tttcatcatc tgttatttcc ccactttcaa
      961 ataattcttt cgtcattatt gaggctacat ctgaactagt aaatgaagat acaaatgcta
     1021 ttcctgctat tccaggtata ccaagcagag gtctaagtaa tggattaaat atctttgatg
     1081 ctgctttcat tcctcccata gattcaacta catctataag acctacagca aaacagacag
     1141 ttggaatcaa agtcagagca aataagaaac catcttttgc tcccgttcct cctttaccca
     1201 taaagtctac accatctgca atagtaccaa atgaacctgt cagattagca aaatctaatg
     1261 ctttaaggaa gccttgacta tctttaaata ctcctgaaaa taatactatc aatacaaata
     1321 aagataaata ccctttccat gttaccttat tatctccagc tgttacatcc atataaaatc
     1381 cccctttata cctcaaattt atctaagttt aaaataactg tagaaattaa tttcaccctc
     1441 tcaaacatag aatctactac tgcatattct cttgatgtgt ggttccattc tcctcttaca
     1501 ccaaatgaac agattgtagg aacgtttgca atagttaaat aacttgcatc tgaactacct
     1561 ccaagtctct tagcatttaa ttctccaaat ccattttctc ttgacacttc atttacaaat
     1621 gtatgaaatc tcataacatc ttctgtagtt tcaaatggca tcatttcact tataaaatta
     1681 acttctgtag aagttccttc tatgtaagtt tctttacata tctctctaac ttgttcttta
     1741 acattctcca tttcttctac tttttcaaat ctaaggtcta cttttatact acaatcttct
     1801 ggaatagaat tttccactct tccaccctta atcacactta cacttactgt agttccaaca
     1861 tctaaattag tcaatttttg tatgcgtagt atcttattag ccatttcttc tatagcattt
     1921 cttccacctt caaaatcatt acctgcatgt gtttctacac cttttacatg tatattacat
     1981 cctattcttc cttttctacc tatacataaa ctattatcta ctaatccagt ttccatatta
     2041 aaagcacaaa gagcaccttt tgcttccctc aatataacat ctgcaccagt tgaatcttta
     2101 tgacctatct cttcatctcc agaaaatact atttttattg gtctttcttt gtatcctatt
     2161 ttatttaaag ctttaataac gtataaagag attattatac cccccttcat atctaaaaca
     2221 cctgggccat atgcttttcc ttctattatt ttaaatggat tctctccaaa tgtacctgtt
     2281 tcaaagacag tatccatgtg tcctgaaaaa attatcggct ttttatctat attagaacca
     2341 agtgtaccta tcaatgtatt accattttcc ccaacatcta ctagttcaca atccaaacct
     2401 tctttttcaa attcaagctt taatctctca gctaacttgt ttacactttc tttacaatgt
     2461 gtataacttt ctatattaac aatctctttc cataatgaaa ctatttcttc tctattctca
     2521 tcaacaaact tagaaatttc atttaaatat gtattcataa tcttctcctt tattttcaaa
     2581 taaaatgtat ttctttccta aaatctaagt ttattttagg aaatcttaaa agactagtcc
     2641 ttaattacaa aatagcacaa ttattcttgc cagtcaacat atttttcagt cttttttata
     2701 ttttctgatt tttctttcaa ttatcctttt gtaaaataaa ttgacgcgtt ttatctacta
     2761 ttataaaatg aggttataaa ttgattgatt agttacaata ctctcgaaat tttaaaggag
     2821 gttttatgaa atataaaatt ggttttttga caacatctta ttttatgtca gataagttta
     2881 aacaagctct taaagaatta aatgatactt gtgaaattac atttattgag cttaaagggg
     2941 agaatccttt taataattta cctaatatat actatgagca agtagataat tttgatggat
     3001 ttgtatgcag tggtatagtt ccatatagtg aacttataat aaatataaaa gacataaaag
     3061 ttccattaaa ttttttaaag cttgatgaaa gagattttta taaatactta ttcaaattat
     3121 taaatagcca aaaagatatt gactttacaa aatcatttat ggatttttta agagaggaca
     3181 acaattatta tgatatatat tcattaatag actctaataa atgtccatat acaattaaag
     3241 attttaatat ccctcaacct gtatatgact ttaaaaaatt aaatgataaa ttattggaaa
     3301 tacatttaga cttattgcga gataataaga ttaacctttc ttttactaga aattacatca
     3361 ttagttgtga acttaataag cttggatatg attgtattta catggttcca tctacagaat
     3421 caatcctgaa tacatttaaa tctctggtga atgaaataac attacaaaac ttagacaaaa
     3481 ataaatctgc aacttgtata gttacagtaa atagttcaga gtacataaat gaagatttga
     3541 tatttaaaaa tgatgagata caaaatcaaa tatataacac tatattaaat tctctatcaa
     3601 gacatggatt ctatggagtt caggtcaaaa aaaatgatat gaaaatagag atacacacta
     3661 ctaaagaaat attaaacaat atgaccaata actatacaga tttttttatt tcagaagatt
     3721 taaaagaaat taaatatagc ttaaatatag gttggggcat tggaaataca aatattcatg
     3781 cagagcaaaa tgctcgtcag gctaatggta aatctgcttc tttgaatgga aactgtactt
     3841 ttatagtaaa tgatactaat gatacaatag gccctttata ttcaaataaa aattctaata
     3901 atattgaaga tagctcaata gccaataaag ttgcttcttt tatacctcta tctaatacaa
     3961 atgtatctaa aatcatgtgt atgattaatg atagaaattc aaataatgtg tcagcagaaa
     4021 tattagcaga ctatatgaac ataactctta gaagtgctaa tagaatattg agtatcttat
     4081 ataaagcagg aattgcaact attgtaaata caaaactcga taatcaaaga ggaaggccaa
     4141 aaaaaatata taaagttg
//
LOCUS       DAEPOZ010000273         4122 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.273, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000273 DAEPOZ010000000
VERSION     DAEPOZ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.273"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            498..1067
                     /locus_tag="KR440_003339"
     CDS             498..1067
                     /locus_tag="KR440_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBH0700343.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKSIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
     gene            complement(1404..2741)
                     /locus_tag="KR440_003340"
     CDS             complement(1404..2741)
                     /locus_tag="KR440_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBH0700344.1"
                     /translation="MDTYSLISTSINFILILINIFIAFFLYKLTSYFIDFRNHWIFKI
                     ILILGYSIISQMIIWLSDPVNILFTLFGYFILLIICSKSKLIPIISVVMILYPIIIGL
                     NFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQYLSN
                     KIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAILANI
                     GVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLE
                     IEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDN
                     HLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKVNPI
                     NRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTENEFSLFLYIRLF"
     gene            complement(3181..3891)
                     /locus_tag="KR440_003341"
     CDS             complement(3181..3891)
                     /locus_tag="KR440_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0700345.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELEKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVHIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLEP"
BASE COUNT         1598 a          472 c          534 g         1518 t
ORIGIN      
        1 tttattatct tctattacat attttgaaat tgatttaatt actactaaat caatatttta
       61 tccactttca aaacaaacac ctattgaaaa tagaactaat tcaaaaccta catatcagat
      121 taatgtttat atagataatt aaaaaataca aagtttaaca agagattaat ataaataatt
      181 ttaataagta tctttcacat atgttaaatt tactttaatt atattaaagc caatgaatta
      241 ttctttagaa gctccaatgc cagtaaatac aggtaaagaa aaatctttta ctacaattgt
      301 aaattaaaaa atattctaat atttaaagaa atgcaaaagt gtaagaaggg taaaaatctg
      361 ttataataca ttaaaaacta aatttagaaa aatttacata attagaaaaa tgatttgata
      421 aaaaataaag ttaaatatta actaagtaaa tttgaaaaag aaaaaatata aagtacaaat
      481 ttaaaaggag gtaactaatg aaaaagaagt tggaagatat gactttagag gagttatgga
      541 acttgtttcc tatatttttg actactcaca aagaatgttg gaaaaattgg tttaaagaag
      601 aacaatatag actaactgga atattgccaa tgcaaaaaat tgttagaata agtcatattg
      661 gaagtacttc tgtgagtacg atttatgcca agtccattgt tgacatactg attgagataa
      721 ttcctgatta taatatgaaa gatattaaag atttgattga gtcgaatgga tatatttgta
      781 tgtctgagag cagtagtagg ctttcactta ataaagggta tacagtagat gggtttgaag
      841 aacgcgtttt tcatttgcat ataaggtatg taggtgataa tgatgagttg ttttttagag
      901 attacttaat tgaccaccca gatgttgcaa aggagtatga aaaacttaag ttggaccttt
      961 ggaagaagta tgagcataat cgtgatgctt atacagatag taaactagat tttgttaggg
     1021 agtttactga aaaggcaaag gtgttgtatt ctaatagata taattagaaa taggtacaat
     1081 tagatattta tcttatatat cgtattatat tttttaagaa agtaattaat agaaaaaggg
     1141 gttgtttcaa aatggagaga taaatccctt tttcattgtt gaaatgataa aaatatattt
     1201 gatttcatgt aaaaaagagt gcctcatcaa ctaaaaagtt tattttgaga cacacacatt
     1261 tttattaagg taaataaaaa tatcacattt gattaaattt taagttaatg aatatatgtg
     1321 taaatattta attataagtt attataattt tacatagtat aaaacaatct aatttgtatt
     1381 atataagata atctaatttt agactaaaat aatctaatat aaagaaatag agaaaattca
     1441 ttctcagtat aagagatatc caattttcca ttgtacttat taacaatttc tctaatattc
     1501 tcaataccat atccgtgaga attactatct tttttatctg aaataaatag ttcttgtttt
     1561 ctattgatag gatttacctt gttgtttgtc aataaatagc taaaatactc cttctcacaa
     1621 cgtgccttta ataccacttt tctatttgac aaatcaggaa ttttcaaaga tgcttctata
     1681 gcgttatcta aagaatttga aaaaatagta cataaatcta taggttctat acccaagtga
     1741 ttgtctatat ctatattaaa gtgcacatca acaccatttt cttccaaact tgtatgacga
     1801 agatttaaaa ttgagttaag agcttggttt ttgcaaaatt gtttgccact aagagtacct
     1861 atacctattt ttaactgatt aaaatacttt ttagctccag caatatcttc aatttctaaa
     1921 tacgattcaa tcatttgaaa gtgattattc atatcatgtc gcattttgcg tatttgaatt
     1981 tgttgatttt ctagtaagtt atagtatttt tcttttaggt gatttgcttg tttttctatc
     2041 ttcatcttta cattttcaat caatggctgt aaaagaagta tcacaccgat atttgccaga
     2101 attgctgaca agatgattag ataagtgcca taaggtaagt tgcctaagat aatattctta
     2161 ttactaccca tagggattaa attagtaggt ggcagaataa tagcaattaa tattgataaa
     2221 aatgaagata tacataccat atcaataaaa atccatattt tatttgataa gtattgtttt
     2281 atattgacca atctattctt taggaaaata tgtattaaaa accaaaataa taatttaatt
     2341 agagcagaaa ttatctggac tattgaaagt attaaaatat attcttttga gtgtataagt
     2401 tcctcatata tagggttgtt tataaaaaga aaattaagcc caattataat aggataaagt
     2461 atcatgacaa ctgatattat aggaattaat ttgcttttgc tacatattat aagtagtata
     2521 aaatatccga atagtgtaaa caaaatattt acggggtcgg aaagccatat tatcatttga
     2581 ctaataatag agtagcccaa aatcaatatg attttgaaaa tccaatgatt cctaaagtct
     2641 ataaaatagg aagtcaactt ataaagaaaa aaggctatga aaatattgat taatattaga
     2701 ataaaattta tgcttgtaga gattaaagaa taagtgtcca tttaatatac ctttctatta
     2761 attaagattc tggaaactaa gttttaaaaa attaaatctt aaaaattaaa attctggaaa
     2821 ttagatttca aagaattgat tttgaaaaat aaaattctag aaattaagct tcaaaaaatt
     2881 gagttttgaa agattgaaat tttaaaaatt aagtttcaaa aaattaaatt tgaaacaaaa
     2941 ctctaaaaac taagcttcaa aaaattaaat tttgaaagat tgaaattcta gaaactaagc
     3001 tttaaaaaat taagtttcaa aaaaactcta aaaactaagc ttcaaaaaat taaattttga
     3061 aagattgaaa ttctagaaat taagcttcaa aaaattaaat ttgaaaaaac aaaactctaa
     3121 aaactgagct ttaaaaaatt aagtttcaaa aataaaattc tagagattga acttcaaaaa
     3181 ttaaggttct aacatatacc ttgtaaattt tcctacaacc tcttgatatt ttttacggct
     3241 tataggaatt ttttcattct gaactttggc ataagtttga gttatactat ctacatggtt
     3301 tagattgaca atataccttt gatgcgtacg tataaaatta gagggaagaa cagtttccat
     3361 atcatttaat tttccgtagt attctaatgt tttatcaaag gtaactacag ttaattttct
     3421 tagctcgctt ttaaagtaga ggatatcttt tgtattaatt ttacaaagat tagttccaat
     3481 atgaactgta ataaagtttt ctttccattg ttctaatttt tttagtgctt cttttaatac
     3541 tttacatatt ttttcagttt tataaggttt taagatgtag ttcaaagctc ctacatcata
     3601 cccatgaaat acatagtcag aaaaacctgt gacaaagatg attatagtat cttttgaatg
     3661 ttttcgtata gtttttgcta tgtcaacgcc attacttttt tcaagttcaa tatctaaaaa
     3721 aataatatct atgtaataat tttcgtgttc tagattgtat actagacttt ctccatttga
     3781 atattctttt atttggtagg ggtagcccca caaatccatt tctcttgtaa tgattttatt
     3841 cagcatgttt ctttgttgtt tttcatcatc acaaatacaa atcgaagtca attttttatc
     3901 tcctttcaag ttttatattt atatagttaa gtatataata tattttgcta cctagatata
     3961 taaaatgaaa taacaattat atccagatag caaaataaaa ttttttatat tagtatactt
     4021 acacttaaaa ctattctatt aaacttattc tgaagtgcta atgattttct tattaaaaaa
     4081 tctaataacc tttcttatta taaaatacca atgcttttat ta
//
LOCUS       DAEPOZ010000274         4096 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.274, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000274 DAEPOZ010000000
VERSION     DAEPOZ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.274"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            243..647
                     /locus_tag="KR440_003342"
     CDS             243..647
                     /locus_tag="KR440_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HBH0700346.1"
                     /translation="MDKTLKKDLNQEYTESKSLVMNKSKYMNICFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
     gene            664..1260
                     /locus_tag="KR440_003343"
     CDS             664..1260
                     /locus_tag="KR440_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700347.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNSDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            1297..3891
                     /gene="clpB"
                     /locus_tag="KR440_003344"
     CDS             1297..3891
                     /gene="clpB"
                     /locus_tag="KR440_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBH0700348.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSDDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
BASE COUNT         1726 a          400 c          721 g         1249 t
ORIGIN      
        1 tatgaaaggc tgccatatta aatagattta aattttttta aaaaatttat aaatattatt
       61 caaaaatcct gccaataaat tggaaatact aaattaaaaa aataataaag tttagcttat
      121 agctaaacaa aagtttactt ataaggtaaa ctttttgcat tttcagtttt gacatacata
      181 tttttttgta atatatttat attatcaatt agagataagg agagtgagaa gagatattta
      241 atttggataa gacattaaaa aaggatttaa atcaggaata tacagagagc aaaagtttag
      301 ttatgaataa aagtaagtat atgaatattt gttttaagaa ttatattaaa gtagtagaag
      361 gaatgtatac taagagggat ttaattccat taaaattaat aaactctatg gataaaaata
      421 ataagattag aaaaacatta tatgaattat cttgtgaatt tgaatatcca aaaacttctt
      481 tatcttcatt gtttacggag ttgaaaaaaa tggatttttt acaaagagtg aaaaatggtg
      541 agtatatgat aaatccacat atagcatata aaggtagtag agctgataaa gaaagtttat
      601 tagcagaata taatcagata aaaagaccta ataaggttag taaataagga agaaggtttt
      661 tatatgagtt taaaacttga tagaaatgtt ctacagtggt ttgactatgt atttgagaat
      721 gaaaaaacta gtttgagaca ctataatttt aattgtacac taaaggagat tagttctacc
      781 agtttgaaca aagtagcttt tattttagaa aaaaataact ctaagtactg gaagctttat
      841 tttgaaatac cagctgaagt tactttaaaa cttaagcaaa acattcatcc tttatttagg
      901 gaatatatat acgaacaaat atcactgtat aacaataatc aaatttacaa ttttgtaaat
      961 tcaaacatat taaaagtgtt taataatatt gctatttatc aatataatat tttagaaaat
     1021 ctatacacta ttgattttaa aaagagtttt atagataaat gtcagtattt attaataggt
     1081 gaaaaaagac ttatagatga agacttgtat ttaatagcaa aatcaaaaga agtattcgat
     1141 ttttttaatt cagatggaac atttaattta acattgagtt tcgatataca aaaaaacgag
     1201 aatttacttg atagtctttt agagcttaga aaatcaataa ttataaatga gagaatttaa
     1261 tttatttgga tataatatta aaatggaggg atttatatgg acgtagaaaa aatgacttta
     1321 agagttcaaa aaagtttaaa tgaggcttat aacgaagcag taaaaaatca taaccaacaa
     1381 gtagatgtaa ttcatctttt ctcagcactt attaatcaag aggatggatt gataccaaat
     1441 ataatcgaaa aaatgaatat atcaattgat tctttgagaa atactgtaaa ctttgaaatt
     1501 gataagcttc caaaagtata tggagaaggt gctgactcac aaggagtatc agctactaga
     1561 aaaataaatg aggtgcttat aaaggcagag agtatttcta aagaatttaa agattcatat
     1621 ataagtgtag agcatgttat gcttgcaatg atggaaacag aatcaaaatc agctgttggt
     1681 aaaatattaa aacaatataa tataaataag aatgactttt taaatatttt atcacaagtg
     1741 agaggtagtc aaagagtaga aacacaagac ccagaaggca catatgatgc acttgctaga
     1801 tatggaacaa acttagtaga tttagccaag aaaaataaat tagaccctgt aattgggaga
     1861 gatgaagaaa taagaaggat tataagaatt ttatcaagaa gaacaaaaaa taatcctgta
     1921 ttgattggtg agccaggtgt aggtaaaact gcaattgtag aagggttagc agagagaata
     1981 gtaagaggag atgtcccaga aggtttaaaa gataagattg tattctcact tgatatggga
     2041 gctttaattg ctggtgcaaa atatagaggt gaatttgaag aaagattaaa ggcagtttta
     2101 aaagaagttc aaagttctga tggaaagatt atactgttta tagatgagat tcatactatc
     2161 gttggagctg gtaaaacaga aggttcaatg gatgcaggta atcttattaa acctatgctt
     2221 gcaagaggtg aattgaactg tattggtgca actacttttg atgaatatag aaagtatata
     2281 gaaaaagata aggctcttga aagacgtttt cagcctgtaa ttgcagaaga acctactgtt
     2341 gaagacacaa tatctatact tagaggttta aaagaaagat ttgagataca tcatggagta
     2401 agaatacatg ataatgctat tgtagcagca gcaaaacttt ctcatagata tatacaagat
     2461 agatttttac cagataaagc aattgattta attgatgaag ctggagctat gattcgtagt
     2521 gaaatagatt cattgccaac agaattagat gttgttagaa gaaagttatt tacattagaa
     2581 actgaaagag aagcattact taaagaaaat gatgataaaa gcaaatcaag attggatgat
     2641 attcaaaaag aaatagcaga gttaaaatct aaaaatgatg aaatgacagc aaaatatgaa
     2701 aaagaaaaga gtcaaatatt agatattaaa aacttaaagg ctcaattaga cgaagctaaa
     2761 ggtaaagctg aaaagtatga aagagaatat gatttcaaca aagcagcaga ggttaaatat
     2821 ggtgaaatac ctaagttaga agaacaaata aagcagtacg aagaaaatat gaatgatggt
     2881 tctgaaaatt ctcttttaaa agaagaagtt actgaagaag aaatatctag cattgtatca
     2941 aagtggacag gtataccagt aactaaacta gttgaaggag aaagagaaaa attacttaaa
     3001 ctagaagatg aacttcataa aagagttatt ggacaagatg aagcagttac agcagtttca
     3061 aatgcagtaa tacgtgcaag agcaggatta aaagatgaaa gaaaaccaat tggttcattt
     3121 attttcttag gccctacagg tgttggtaaa actgaacttg caaaaactct tgctagaaat
     3181 ttattcgata gtgacgataa tataataaga atagatatgt ctgaatacat ggaaaaacat
     3241 gctgtatcaa gactagttgg acctcctcca gggtacgtgg gatatgaaga gggtggtcaa
     3301 cttacagaag cagttagacg tgctccttat tctgtaatac tatttgatga gatagaaaaa
     3361 gcacatgagg atgtatttaa catgttctta cagatattag atgatggtag acttactgat
     3421 aataaaggta agacagtaga ctttaaaaat actcttataa taatgacttc taatatagga
     3481 agtaattatc tattagaagc aggaggaaat ataacagaga ctactaacaa tttagtaatg
     3541 aatgaaatga agcatagatt taaacctgag tttttaaaca gagtagatga tattataatg
     3601 tttaagcctt tagaccaaga aaatatcaag aaaattatag atatatttat gaaagattta
     3661 aaaaatagat taaaagaaaa agatattact attgaagtta caaattcagc aaaagatgtt
     3721 atggtgagag aaggatatga ccctgtatat ggagcaagac ctcttaagag atatatagga
     3781 aatacattag aaactataat tgcaaagaaa cttattgctg gagatgtgta taatggatgt
     3841 actatagtta tagatggaaa agatgaaaat attgaagttt tagttaaata ataaaagaat
     3901 cagttgagca taatatacta aatattttgc tcagctgatt tttatttata aactatagga
     3961 aaagaaatat tttatcaaat taataaaaca tttttaagtc aatatgtcca aattaaattt
     4021 aactcaaagg aagtatttta gtaagtttct tataatataa tcataatttg aatataatta
     4081 taaaaaacta ctgttc
//
LOCUS       DAEPOZ010000275         4042 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.275, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000275 DAEPOZ010000000
VERSION     DAEPOZ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.275"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            122..1162
                     /locus_tag="KR440_003345"
     CDS             122..1162
                     /locus_tag="KR440_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBH0700349.1"
                     /translation="MHLSKKIKKLFLDDKGYYFNSNWEDIHIGIYDTKLGDSLKYITN
                     EYIENMERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYLCLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSLQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
     gene            1301..1753
                     /gene="tsaE"
                     /locus_tag="KR440_003346"
     CDS             1301..1753
                     /gene="tsaE"
                     /locus_tag="KR440_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBH0700350.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            1753..2469
                     /gene="tsaB"
                     /locus_tag="KR440_003347"
     CDS             1753..2469
                     /gene="tsaB"
                     /locus_tag="KR440_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBH0700351.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            2459..2935
                     /gene="rimI"
                     /locus_tag="KR440_003348"
     CDS             2459..2935
                     /gene="rimI"
                     /locus_tag="KR440_003348"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBH0700352.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKCIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            2941..3957
                     /gene="tsaD"
                     /locus_tag="KR440_003349"
     CDS             2941..3957
                     /gene="tsaD"
                     /locus_tag="KR440_003349"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBH0700353.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSNKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
BASE COUNT         1717 a          379 c          756 g         1190 t
ORIGIN      
        1 tctacaatat acagagcgaa tttataataa acaaaaaata aagttattat taaaaaacta
       61 taaagactaa taatcaaact tttaataata actaatttaa taatgatagg ggtgtatatt
      121 catgcattta tctaaaaaaa taaaaaaatt atttttagat gataaagggt attattttaa
      181 tagtaattgg gaagatattc atattggtat ttatgataca aaattaggag attctttaaa
      241 gtatatcact aatgaatata ttgaaaatat ggaaagggta aaatacgatt atcgtgaatt
      301 aaaaaatata gaaaatcaaa ctgatggtat gtgtatttta gcaataaggc agaattacaa
      361 ggcattagag tatgtaaaaa atcaaaccga agaattatgt ttagaggcaa taaagtataa
      421 ttataaagca cttgaatatg taaaagaaca aacagaatac ttgtgtcttg aagctataaa
      481 aaaagattgt aatgcactta agtatgtacg aaataaaaca gaagggctta ttataaaagc
      541 aataagtcat tcttccaata tagatgttgt aagtatacta aaggctttag agactcaaac
      601 aagaaggata tgtttagaag caattaaaaa agatggcagg tgtcttgcat acgttagaga
      661 acagagtgaa gaattatgta tagaggccat taagcaaaat tataaagcat taaaatatgt
      721 aaaaaatcaa acggaaaaaa tgtgtataga atccgtaaga caaaatggta tggcattaca
      781 atatgtaaat aaacaaactg ataaaatatg tatagaagct gtcaaacaag atggaagaag
      841 tctacaattt gtcaacaata agacagaaga aatatgtata aatgctataa gatatttaaa
      901 taaaaaatat aatattaaag atgtactaag ttacatagat aagtatacag aagatatatg
      961 tatagagatt gtaagacaaa atggaaaaat gcttatgtat ataaaaaatc aaacagaaaa
     1021 aatgtgtatt gaagcagtaa aagagaacta taagtctctt aagtatgtaa aagagcaatc
     1081 tgagagaata tgtaaagaag cattgaagca aaaccataaa gcaaaagagt atgtaaaaat
     1141 tgctattgat gattgtatat aaggtgtaga tacagccatc aatagtgaaa gataaaaaaa
     1201 gataaagaca agtactttat aataatctag gcttatacta taattaatta agcagtgata
     1261 taaattcaca tgacgattat atcaaaatgg agatgaattg atggcaaaaa tatatttaga
     1321 aaatgaaaat aaaactagag aaataggata taaattaggt aaacttttaa aagaaggcag
     1381 tgttatctgt ttagtaggag atttaggagc tggtaaaact acaatgacac aaagcttggc
     1441 agattcatta gggatagaag attatataac aagtcctact tttactataa taaatgaata
     1501 tgaaggaaaa atacctttgt atcattttga tgtttataga ataggtagta gtgatgaaat
     1561 gtatgatatt ggttatgatg aatatgtaaa ttcaaatgga atctgtataa tagaatgggc
     1621 taatctaata gaagatatac ttccaaaaga atatttgaat atagaattga gatataaaga
     1681 tgaaggaaga gaaatgatat taactccaaa aggagagttt tataaagaga tagttgagga
     1741 gttgattaaa taatgaaaat actaggtatg gatacgtctt ctatggcagc aagtgtagca
     1801 gttgttgaag atgataactt aatatgtgag tttacagtaa acaacaaaaa aactcattca
     1861 caaaagctta tgccaatgat agaaaatatg ttgagtatga gtgatttaag tataaaagat
     1921 atggatttat tagctgtatg tataggtcca ggttcgttta caggacttag aataggtatg
     1981 gctacagtaa aagctatggc ccatgtaaat aatataccaa taatagcagt aaattctttg
     2041 gaatctctag caaataacat aaatttctgt gatagaaaga tatgttgtat attagatgcg
     2101 cagaaaaatc aagtttattc agctaaatac aagtttgaaa atggagagat gatagaatta
     2161 gatggtgttg atgttgtaga gtttgatgct ttagtagatg aaattgtatc aactaatgaa
     2221 gaatttataa tagttggaga agctgtatac aaatataaag acaagttaga gaatataaaa
     2281 aatataaaaa taccttcacc agctaataat gtatctaacg caggaagctt atgctcttta
     2341 gcattaaata aatataataa aaatatagat gtacatactt gttatacgat aaatcctatg
     2401 tatatcagaa aatctcaagc tgaagtacaa tatgatgaaa aaatgaagag gctaaataat
     2461 ggaagataat acaaaactaa tagtagatga tataaaaata gaagaaatga ctacagaaga
     2521 tatagatgaa gtttttgaag tagaaaaaaa ttgttttgaa gattattggt caaaagagtc
     2581 ttttagaaaa gagttgtcta atgaagtagc aaggtatata gtagctaagt taaatggaaa
     2641 aattgtcgga tatgtgggaa tttggcttat acttgatgaa gggcatataa ataatgtggc
     2701 tgttcatagt gactatagag ggaaaaaaat aggggacaag ttgataaaat gcatagttga
     2761 tttatgtaaa gataataata ttgcatctat gacattagaa gttagagcat caaataaaat
     2821 agcacaaaat ttatatagaa aatatggatt taaaatgggt ggaataagaa aagaatacta
     2881 cagtgataat aaagaagatg caattataat gtggaaccaa ttaaaagagg tgtagagttt
     2941 atgagtgata taataacatt agctatagag agtagttgtg acgaaacagc tgcatcagtt
     3001 ttaaaaaatg gtagagaagt actttcaaac ataatctcta ctcaaatcga gacacataaa
     3061 aaatttggtg gtgtagttcc agaagtggcc tcaagaaaac atgtggaaaa tattgatata
     3121 gttgttcaag aagctttaga caaagcaaat ataggtttta atgatataga ccatatcgca
     3181 gttacatatg gacctgggct agtaggtgct ttattggttg gtctatctta tgctaaagca
     3241 cttgcatata ctttgaatat tccacttgta ggagtaaatc atatagaagg gcatctaagt
     3301 gcaaactata tagagcataa ggatttaaaa cctcctttta taacattaat agtatctgga
     3361 ggtcatactc acttggttga agtaaaagat tatggcaagt atgaaatttt aggaaaaaca
     3421 agagatgatg catctggaga agcatttgat aagatttcaa gagctatgaa tttaggctat
     3481 ccaggaggtc ctattattga taatctggcc aagaatggaa ataaacatgc tatagaattt
     3541 ccaagagcct atttagaaga agatagctat gattttagtt ttagtggatt aaaatcttct
     3601 gtattaaatt atctaaatgg aaaaagaatg aaaaatgaag aaatagtagt tgaagatgta
     3661 gcagcatctt tccaagaggc tgtagtagag gttttatcta ataaagcact aaaagcagta
     3721 aaagacaaag gatacaatat cataactctt tctggtgggg ttgcttctaa ttcggggctt
     3781 agagctaaaa taacagagct tgctaaagac aatggtatta ctgttaagta tcctcctcta
     3841 attttatgca cagataatgc agctatgata gggtgtgcag gatattataa ttttataaat
     3901 gggaaaacgc atgatatgag tttaaatgca gttccaaatt taaagataaa tcagtagagt
     3961 tagatgaatt tgtaaatata aaagtattgt ttcaaaaaag taagtagaag tgaaattaaa
     4021 tataataaaa ataaatatag ta
//
LOCUS       DAEPOZ010000276         4039 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.276, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000276 DAEPOZ010000000
VERSION     DAEPOZ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.276"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(356..841)
                     /locus_tag="KR440_003350"
     CDS             complement(356..841)
                     /locus_tag="KR440_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBH0700354.1"
                     /translation="MISELMLKNFGKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
     regulatory      complement(924..1017)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1373..1756)
                     /locus_tag="KR440_003351"
                     /pseudo
     CDS             complement(1373..1756)
                     /locus_tag="KR440_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      1983..2080
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2135..2311
                     /locus_tag="KR440_003352"
     CDS             2135..2311
                     /locus_tag="KR440_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700355.1"
                     /translation="MFGNKMDKCTHVLTAYISGSYDYCNFLDTQLDDFILEYGESVVE
                     SCLHQVMVLVSKYN"
     gene            2537..2737
                     /locus_tag="KR440_003353"
     CDS             2537..2737
                     /locus_tag="KR440_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBH0700356.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     gene            complement(2901..3200)
                     /locus_tag="KR440_003354"
     CDS             complement(2901..3200)
                     /locus_tag="KR440_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700357.1"
                     /translation="MGFYINYVVCKVKILFSSFSKIILFYINYVVCKLFITLKAILLK
                     TQIIVTTHSPHILQIDSKEEMIVLDMAESGNVYKKELELGEYGVLGWTNEGLYLH"
     gene            3422..3604
                     /locus_tag="KR440_003355"
     CDS             3422..3604
                     /locus_tag="KR440_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBH0700358.1"
                     /translation="MFKFYNNATLMFLSGTNIKTISERLGHADIKITMNSYSQFLEEM
                     DREAFENLSKILFIVF"
     gene            3675..>4039
                     /locus_tag="KR440_003356"
     CDS             3675..>4039
                     /locus_tag="KR440_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HBH0700359.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSL"
BASE COUNT         1454 a          508 c          449 g         1628 t
ORIGIN      
        1 attttccccc tcataaaaat ttttaactag ttcattataa tatatatata actaaaaatt
       61 acaaattttt tcaagtttct tatatatctt attatcttta atattatttt tattatcata
      121 acttttactt tttttaaaat ttttcaaagt agtattaatt tatgatactt ggtgtataaa
      181 gtataagtta aatctacttt caattaaatt taccatttat aattacttta taataaaaat
      241 aaattagtta atttattctc tttagttctg ttataatgtt tctacaaaaa agaatcccat
      301 taatgggatt cttttttgtg atttattaac atatttaaag ctcactagta tcgtattata
      361 aacttaaatg ctcttctatg tattgattat atacatatcc agaatatcgt ttttcatttt
      421 tattgatatt aactcttgcc cattttccaa cgctataaca tacttcaact ttaaaaccat
      481 cagggattgt agttataact ttagattcta atgttctatc atttctaagt tctaaactta
      541 atccatttgt tcttatattt cctatgtaat tcttttttct attttttatt gtatcattac
      601 tattgttttt agcattactt aatctttcta aatatttata aagtaaccag cctttgtcat
      661 aattactctc aacttctgcc catattccat ctacacattt taacttaaca attttacctt
      721 ctggtatact ttttaatttc aaactattta tgtctggttt ttctcttaaa ttcaatttaa
      781 ttttagtttt aaaattgatg ctagatttgc caaaattttt taacattaat tcagatatca
      841 tcttaagccc tcctcttgcc aaatattaag ttattaaaat atataactct aaaaatactt
      901 ttaacaaaaa gaattattat ctttttggca actggctgac atgaccttta ttacataaag
      961 attttagtcc ctatagtttt gcgtcattag atttatctaa tattgcctat atctgaataa
     1021 taacataatt tgaacaatta aaaaataata ttacctcaaa ttttatatta tttaaatata
     1081 aattaaattt ttaattaata ataatcaaaa aatatcttta tatttattca attactgttc
     1141 tttatagtca atataaaaga cagtaattta tctatagtgt tacttatatt ttatatatat
     1201 ggctattttt caacatttta caatatatat aacatttatt tttttaactt ttgtcagtaa
     1261 aatgtcagca agacacttta tccacaattt ttatataagt tttccacaac ttatccccag
     1321 tattatccac ataaaaaagg tagctcttat attattagct accttttatt tattaatctt
     1381 catatttcca gctttttatc caacttccta tttctattat tttttcttca gttatttctt
     1441 ctccatcatg taattttgaa attaattctg ataaaggatt gttatgtaaa cctctaaaaa
     1501 ctctttttcc ctctattttg agatattctc tctcttttat taatactgta tagtgtacat
     1561 gcctcccaat tctctatgat attaaaaaac cttttttggt taatctagct aaaagtataa
     1621 ttgtagttgt atgcttccaa ccatatgttt tttctatttc atctgcaaca tcttttgata
     1681 ttacagtttt ataaccagta ttccatatgc atttcataat ttttaattct gtatctggta
     1741 attttttatt tagcataata tattccttct ttcaatttat gtcacttctt caaatttatc
     1801 tagcttaaat tataatagga gttttttaga acaaaaacta tagttaattt ttaccaggta
     1861 ctctacgtgt ttagtctgtt ttttaagtgt aaaataatgt tcatatctat aaatttattt
     1921 aactttcaaa caatcctgtt gaataataac taattctatt gtattatttt agttgtagaa
     1981 taaaactaaa tcggcaaaac tagagaaatt tagtgacgca aagctatagg gactaagact
     2041 tatataaata tcttatgagt tatgtcagcc agttgccaaa gagatagtgt tctttttgtt
     2101 tttatgaaag ttttttaggg ggataataat ttatatgttt ggaaataaaa tggataaatg
     2161 tacacatgta ttgactgctt atattagcgg ttcgtatgat tattgtaatt ttttagatac
     2221 acaattagat gattttatat tagagtacgg agaaagtgta gtagagtctt gtttgcacca
     2281 agtgatggta ttggtaagta agtataatta gatagacatt gatttctaga gaaccctttt
     2341 tagttttatc tacatttata ttaatatata gcaatgtaaa acgcttaaat atatttttaa
     2401 tattaagaat aactaaaatg ttgactaaaa aatataatta agtagacatt tctattaatc
     2461 tatgatacaa tagtatttgt ggtaatacac aattaaatca agtatatctt gatataggtt
     2521 aataggagta tttttaatga ataatggaat agtgaaatgg tttaataatg aaaaaggatt
     2581 cggttttata tcaatggaag gtagagatga tgtgtttgct catttctcag ctatacaaac
     2641 ttcaggattt aaatcattag aagaaggtca acaagtaagc tttgatatag ttaaaggtgc
     2701 tagaggtcct caagcagaaa atataactat attataatat atagattttt cgggaaagac
     2761 tctttttaga gtcttttttt attgttagta tatgtaagat aatcattggt gaagtaaata
     2821 aggtatattg agtaatgttc ttgatagttt atatgctaat gaatttcaaa aaaataagca
     2881 ctcctataaa aagagtgctc ttaatgtaaa tataagcctt cattggtcca gcctaatacc
     2941 ccgtattctc caagttctaa ctctttttta tatacattac cactttcagc catatctaac
     3001 acaatcattt cttctttaga atcaatttgt aaaatatgag gactgtgtgt tgtgacaatt
     3061 atttgtgttt ttaggagaat tgcttttaat gtaataaaaa gtttacatac aacatagtta
     3121 atataaaaca aaattatttt tgagaatgag ctaaataaaa tcttaacttt acataccaca
     3181 tagttgatat aaaaccccaa aataaactta gcatctccaa tgtttacaca tatacaatgc
     3241 tcttaaaatt gcagtgaaca tgcaatagtg caattgataa tacttatcat atattctcaa
     3301 taccttatat ttcaactgct aaacctcaat ttgtcacaaa tatcgctcac tgcaaaattt
     3361 ctatattttt attatatcat agcatataat taatattttc aattaatttt tacatttaat
     3421 tatgtttaaa ttctataata atgcaacttt aatgtttctt tctggaacta atataaaaac
     3481 tatttcagaa agattaggtc atgcagatat aaaaataact atgaatagct actctcagtt
     3541 tttagaagag atggacagag aagcatttga aaatttgagc aaaatactat ttattgtatt
     3601 ttaaagaaga tgacttattc aatatttgat gctagaatat aattaataaa aatttaatta
     3661 taggtgattg tattatgaac attattaaaa gcataacttt actcttaaca ttattaattg
     3721 tgatattaat gacaggatgt tccccaagag aaagtgctag tgaagttgca aaaaaatata
     3781 ttgataagat gaactctggt gactatgaaa cagcatatag tttattaagt aaagatagta
     3841 aaaaaaaaat tagtatagaa aatcttgaag aatttcaaaa tattttagtt agtacaaaaa
     3901 gaataattag ttataaaata ggtaaagaaa aaatcataaa aaaatataaa catgatggaa
     3961 aagaatataa aaatgttgtt aaattagaag aaacttttaa tataaaaaac cttctttata
     4021 aaaaagatga ttctctaaa
//
LOCUS       DAEPOZ010000277         4006 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.277, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000277 DAEPOZ010000000
VERSION     DAEPOZ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.277"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(238..1914)
                     /locus_tag="KR440_003357"
     CDS             complement(238..1914)
                     /locus_tag="KR440_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBH0700360.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIDGYSLEAQKSRMKAFALYNDYEIV
                     GEYEDAGKSGKSIEGRVQFTRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDFGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRVQTMEGRIQKAKEGKWNG
                     GFAPYGYQLIDGKLIINEEEAIAIRTIFDQYVNTSIGANGLSKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYRQVEQDDYLVAEGIHEAI
                     IPDELWQAAQVKLKAQAKKYEHVNKGKNMRTHLLSGIVKCPICGAGMFGNKSIKYKKD
                     GTKYKDFYYYGCKHRLMNRGHKCTYNKQIREELLDDAVAEVIIKLVSNPKFASMIQEK
                     INMKVDTSAIENEIDNYQNELRKSHSTKYKLIEEIDNLDVEDKHYKRRKNDLDDRLYR
                     MYDKIEELESQLIEAKAKKETIEVEKLTGDNIYKVLIYFDKLYKVMNDVERRQLIEAL
                     ISEIQIYEEKQPNGQWLKSITFKLPIIDEDLNISLENDEQVETVAKIIKQ"
     gene            complement(2015..2167)
                     /locus_tag="KR440_003358"
     CDS             complement(2015..2167)
                     /locus_tag="KR440_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700361.1"
                     /translation="MDNDWNGLADLIANLITKYAGVLDLENLPNPTPVKNTEDKNKFD
                     IAKSTD"
     gene            complement(2656..3066)
                     /locus_tag="KR440_003359"
     CDS             complement(2656..3066)
                     /locus_tag="KR440_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBH0700362.1"
                     /translation="MAKEYYLYIRGQKVKVSEDIYIVYWREKEHEKYLEQVDRKNHLL
                     FFSALDHDGHFTDNIVDESVDVEKIVETQMMIEAVRSAITRLNAEERDIIERLYFNDE
                     TVRSVAKLKSITHPALIKRRNKILEKLKKFIEEL"
     gene            complement(3590..>4006)
                     /locus_tag="KR440_003360"
     CDS             complement(3590..>4006)
                     /locus_tag="KR440_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0700363.1"
                     /translation="EYLKINDTLEDILKKYEILANQKKITVNNYILDENVYIGKTALK
                     IILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIFDVKFDLNKENS
                     NGLGLYIVSNILNNYNIEYKALQDEEFFIFKIKIFS"
BASE COUNT         1212 a          768 c          439 g         1587 t
ORIGIN      
        1 ttcaactata gattatgtaa attatcctat gaaaaaaata agttgtataa aatctggcaa
       61 ctgtagattg acgatttctt ttaaaattac aattataata agttttatag aattaaaaat
      121 aaaactttag agatttttga taacattgta gactaaattt gccaccgaaa ttactttaca
      181 gtggcaaatc ggtggcaaat acaatatatt attagtatat aatctatata aacattacta
      241 ttgcttgatt atcttagcta cagtttcaac ttgctcatca ttttccaaac ttatattaag
      301 atcttcatca atgataggta gcttaaaagt aatggatttt agccattgtc cattaggctg
      361 cttttcttca taaatttgaa tttctgaaat gagtgcttct attaactgcc tgcgttcaac
      421 atcgttcatg actttataaa gcttgtcaaa ataaatcaat actttatata tgttatcacc
      481 agtaagtttc tcaacctcta tcgtttcttt ttttgcttta gcttctatta actgactttc
      541 taattcctca attttatcgt acatacgata aagcctatca tctaaatcat tttttcttct
      601 cttataatgt ttatcctcta catctaaatt atctatttcc tcaataagct tatactttgt
      661 agaatgactt tttcttagtt cattttggta attatctatc tcattttcaa tagcagatgt
      721 atcaaccttc atgttaattt tttcttgtat catagatgca aatttagggt tgcttactaa
      781 cttgattatt acctcagcaa cggcatcatc taacaattct tctctaattt gtttattata
      841 ggtacattta tgtcctctat tcataagtct atgcttacaa ccatagtaat agaagtcttt
      901 atacttcgtc ccatcttttt tatattttat agatttattc ccaaacattc cagctccaca
      961 tatcggacac tttactattc ctgataataa gtgtgtcctc atgttcttgc ccttatttac
     1021 atgttcatac tttttagctt gtgctttcag tttaacttgt gcagcctgcc acaattcatc
     1081 tgggattatt gcttcatgaa tgccttctgc tactaaataa tcgtcctgct ctacttgtct
     1141 gtattcattc cttgtaccat gaactttttc taaagttctt cttccaaatg ctattttccc
     1201 gttatacaca ggattcttta atatttttct tataagacct gcgtcaaaca atggattttt
     1261 cccattttgt cttggtattt tccttatacc atgattttct aaatatttag aaagaccatt
     1321 agctccaata cttgtattta catattgatc aaaaatagtt cttattgcaa ttgcttcttc
     1381 ctcatttatt atcaatttcc catcaataag ttgataacca tacggagcaa atccaccatt
     1441 ccatttaccc tcttttgcct tttgaatgcg accttccatt gtttggacac gaatgttttc
     1501 tctttctatt tcagccactg ccgataaaac agaaatcatc aatttacccg catctttaga
     1561 tgaatcaatt ccatcttcaa cgcaaataag atttactcca aaatcctgca ttatttgtaa
     1621 agtcgaaagt acatcagcag catttcttgc aaatctcgat agtttaaata caagaacgaa
     1681 agacactcca tcctttccgg attttatatc ttccatcata cgagtaaact gaactctacc
     1741 ttctatagat tttccagact tacctgcatc ttcgtattca ccaacaatct cataatcatt
     1801 atagagagca aaagccttca ttcttgattt ttgtgcctct aaagaataac catctatctg
     1861 tatggatgta gatactcttg tatagagata tacttttatt ttttcttttg acatagcatt
     1921 aacctcaata tccttattat atatttatat atttttacca ataaaggttt ggactttaat
     1981 ctcaagtata ttatagcact tttatttctc tttctcaatc cgtgctttta gctatatcaa
     2041 atttattctt atcctctgta ttttttactg gagtaggatt tggaagattc tctaaatcta
     2101 aaactccagc atattttgta atcagatttg ctatcaaatc agccaatcca ttccagtcat
     2161 tatccactaa tacacctcct ccactttaaa ttctttataa ccaaatatta tttttcttac
     2221 taagttttat gacattggta ggtgcattgg ctgctacata ggaatctcac ctccgcccct
     2281 tgtttcagac gaggcagctt aaactattga agtatcatta tcaccactta catcatcgaa
     2341 ataagctgcc acgcttatct tttatgtatt cctatctatc gctcattttc ttctaacctc
     2401 ggcattatgc cggtattcat ttgaaccgat tttcatcagc agaaaagtct tggcgtaggt
     2461 cataactctc agtaagtaga taaagtccta ccttggtcta ttcagttgtc aaagatcaat
     2521 atttgaaagg gactgttttc tttacatttt cctatttgcc ataagcaggc gtatctccta
     2581 ctcaagaaag taaaggaggt caaactcccc ttcactttac ataaggaaaa tttgccccct
     2641 ttggttacca aagttttaaa gttcttcgat aaattttttc agcttttcaa gaattttgtt
     2701 tcttcttttg attaaagccg gatgtgtaat acttttgagc tttgctactg aacgaacagt
     2761 ttcatcattg aaatacaagc gttcaataat atctctttct tctgcattga gccttgttat
     2821 agcacttctt actgcttcaa tcatcatctg tgtttcaaca atcttttcta catcaacact
     2881 ttcatcaaca atattatctg taaagtgtcc gtcatgatcc aatgctgaaa aaaagagcaa
     2941 gtggtttttc ctgtccacct gctctaaata cttctcgtgt tctttttctc gccagtagac
     3001 tatatatata tcttcactga cttttacctt ttgtcctctg atataaagat aatactcttt
     3061 tgccataaag tttcctccat ctctttttta tctgcttgtt tttcagataa aaaaggagga
     3121 cttctgcatc ttggcataag aagtcctctg aaatgaaaga ataaaaatat ttctttatat
     3181 gaacttgatt gtttcaattc gcacctgaaa caatcatcta ctatacatat tataaaaaag
     3241 aggaatgtat tgaattctaa atatttctaa cattaaacgt ttagatgctt ccatatttat
     3301 ttcctccatt ttatcaataa tctaaataac catcatattc atctactact tatataattg
     3361 agttttatta aactttttaa gtttctattc tatcaaatat ttaaacagtc tctgcatccg
     3421 aaaagactaa ctcatatagt tttactaaca atattttcat tagcgtgttc aaatcctgat
     3481 ttcttgtatc aattattttc tgttcacttt caataagaaa aaagccttta cttccatggc
     3541 taataataag tctctatttt tcaataacta ttgcaaaatt atatcttgtt taagaaaata
     3601 ttttaatttt aaagataaaa aattcttcat cctgcaatgc tttatattct atattgtaat
     3661 tattcagaat attacttaca atatataaac ctaatccatt actattttcc ttatttaaat
     3721 caaatttaac atcaaatatt ttatccatat tcgaaatttt attgttaccg tatgaattct
     3781 ctatatataa ccaatcatta actatcccaa tattaattac cccatttaca tcagtatatt
     3841 ttaccgcatt actaatcaaa ttagatagaa taatctttaa agctgttttt cctatataaa
     3901 cattctcatc taatatataa ttattgacag ttattttctt ttgatttgct aatatttcat
     3961 acttttttaa tatgtcttct aatgtatcat ttattttcaa atattc
//
LOCUS       DAEPOZ010000278         3999 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.278, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000278 DAEPOZ010000000
VERSION     DAEPOZ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.278"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..1786)
                     /locus_tag="KR440_003361"
     CDS             complement(<1..1786)
                     /locus_tag="KR440_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0700364.1"
                     /translation="MDFRKFAYNNIVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELTILGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEIYFPVKAILLTIVS
                     FMILFLITGPMSLKLLRKKSILELLVGTKKPKKEIKSSRLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGIII
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYDEVKGE
                     FSKINFHGFDYKNWQSSAKITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFRLE"
     gene            complement(1786..2532)
                     /locus_tag="KR440_003362"
     CDS             complement(1786..2532)
                     /locus_tag="KR440_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0700365.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PTILLADEPTGNLDSKSTDDVLKLFTRINKEQNVTTLMVTHEVYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            complement(2679..>3999)
                     /locus_tag="KR440_003363"
     CDS             complement(2679..>3999)
                     /locus_tag="KR440_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBH0700366.1"
                     /translation="NAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYILKNYYQDNFYL
                     VQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDHFILLIACNTE
                     DELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLLIDRANIAAKS
                     KKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYSLDDVNEIEGA
                     EALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWINKGYTPVPIS
                     VNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILIDIMKKIKEIG
                     FLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISKIVEMAKAIDI
                     KVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
BASE COUNT         1406 a          645 c          358 g         1590 t
ORIGIN      
        1 tttctaacct aaaactatta tttaatactt gatttaaaaa cagtaaagaa attatgctat
       61 ttataattgc tataaaatac ggtataaaaa acaatgtacc tatctctata ctaacaatct
      121 tattcatatt tttaaaagat agacctattt ttgatagttt tttgtacttt acattatctt
      181 ctatctcgtc tgtatataat ctgaagtata aaaaactaaa aactgctata aataatatca
      241 ctcctataaa aacacccata aattgaagaa ttttactttg ttgtaactct actaaataca
      301 tatctggtaa tgttaaaaaa tatgacctgt tatattctct atccaaatta aaatgttttt
      361 cttttaaatt ttcagttatc tttgcactgc tttgccagtt tttataatca aatccatgaa
      421 aattaatttt tgaaaattca ccttttactt catcgtataa gtcatcactt ataactacaa
      481 ctttactaaa cagtcctgta ggaaaaatta ttttattttt tgattttttt acttctatat
      541 ttaaattttt tatactataa ttttttaatt gttttaaaga atttatatgt tcttgtgaat
      601 tgctactagg aattataatc ccttcatatt tttctaactt tgaactaatt ttatctaatc
      661 ccaattcctt tgataatttg ttataattct ttacacttag taagacttcc ctattatatt
      721 taagtacagg aaaacttaac tccttataat catatccttt gattgcttct ctaatttgtt
      781 gtagttcttc atattctttt tcattattat gctctgaaaa atatgagtaa accattggga
      841 aattattttt agaagcttct ccttgagaac ttacaagtgt tgaagttgtt gtaaatgcta
      901 ctaaagtagc cgataaaagt attgttatta aaaataacaa tcgtgtatta tcctttaatt
      961 tatatatcaa attacttata agtaacatat ttactttgtt cttataaaat ttttctttat
     1021 tttttaaata ctttaatatc aaaatagtaa attgagaaaa aaataaaaat atccccaaag
     1081 taattaatag tgctgttgta tacatacctc catttatttt taacttattg ccaaatagta
     1141 tcatagcata tcctaatata agacatatta caccaattat tccaaatagc cttgaagatt
     1201 ttatctcttt tttaggcttt tttgtaccta ctaaaagctc taaaatactc ttttttctca
     1261 ataattttaa gctcattggt cctgttatca aaaataaaat cataaatgat acaatagtca
     1321 atagtattgc ttttactgga aaataaattt cagttaaatt catataaaac agtttgctta
     1381 aatacaataa aaacaatttt gaaattgata aaccaagtaa tattcctaac atcactgcta
     1441 aaaatcctat tatcaaattt tcaattaata tcaatttttt cagttgtctg ttgctcattc
     1501 caattatcat caatactcca aaatctttca atctatattt caagaaattt cctaaagagt
     1561 acaatataaa aagtagacta aaacctaata ttgttaattc tgaagtcata agagccataa
     1621 atgcagtaga accactctgc attccagatt ttgttataat cggatggaac atagacactg
     1681 caaaaacaaa aaatatcatt atagaaaaaa cagaacttaa aaaatatgct aaatatgccc
     1741 tgaaatttct aactatatta ttataagcaa actttctaaa atccactatc ttacacctcc
     1801 tatttgagac aaaacagcta gtatatcaga atagaaagaa ttactgcttt cagatttttt
     1861 tatttctgta tatatacaac catcttttat aaaaattact ctatctgaat aacttgcact
     1921 atatacttca tgagttacca taagtgttgt tacattttgt tctttgttta tcctagtaaa
     1981 taatttcaaa acatcatcag tagattttga atcaagattt ccagttggct catctgcaag
     2041 aagtatggta ggtttattta taatagctct agcaatagca cacctttgag cttgacctcc
     2101 tgaaatttca taggtttttc tatctaaaat tttatctatt cctaaaaatt tagatatact
     2161 tttagtttgt atattcatat ctttgatact tgccccctct agtgtaagtg gcaacataat
     2221 attttcgcca acagttaaag tgtcaatcaa attaaaatcc tgaaatacaa aaccaagttg
     2281 tttccttcta aattcagcaa gtttttctcc tttcaattta cttacatcat atccatctaa
     2341 tacaacctca ccttcactct gtctatcaac tgtagatatt atatttaaaa gagtagattt
     2401 accacttcca gatggtccca taacagacac aaattctccc ttttctatat ttagactaac
     2461 cttatctaat gctttaaatg gaatatttcc gcaatatgtt tttgatatat ttctaatact
     2521 caatatgttc attttatatt ctccataatt ttttattttt actacaatac cataatatcg
     2581 tgaatatact actttaacca taaaaatacc ctaacaatta acttacatat ttgtaagtaa
     2641 gttgttaggg tatattatag tctaataatt attaatgatt aatcaaattt aaagtttaag
     2701 tgtttttcaa attcatctaa taccataggt ttagcaaata agtatccttg aaccttatca
     2761 caacctatct ctttcaagaa ctctacttgt tcataagttt ctacgccctc acagattact
     2821 ttaatatcaa tcgcttttgc catttccaca atcttagaaa taataatttt tccacgtttt
     2881 gtatctgttg tttcgattaa aaatcctcta tctaatttta aaatatcaaa agaaaggtct
     2941 tttaacagat ttaaagatga atatcccgaa ccaaaatcat ccattgatat aaggaatcct
     3001 atctctttaa tctttttcat tatatcaatc aatatattta aattatcaaa cacaacactt
     3061 tctgttagtt caagttcaat aaactcagga gatatattgt atttacttat caaatcagtt
     3121 atattttcta taaaattatc cctatataaa tgaactctag acatatttac agagattggt
     3181 acaggagtat accctttatt tatccacttg tttaatgttt tgcatacttc ttcaaacacg
     3241 aacatatcaa tatttacaat aaaaccattc ttttcaaata aaggtacaaa atctataggt
     3301 gaaataaacc caaattctgg actattccat cttatcaagg cttctgctcc ttcaatttca
     3361 tttacatcat ctaaactata cttaggttga taatatacta taaattcacc tttttctaaa
     3421 gctttattca tattatcttc caatcgtttt tccttgtaca atcgatttct tgtatcttca
     3481 ttaaaaaacg aatatgaatg ttcatatttt tcctttttac tttttgctgc aatatttgct
     3541 ctatcaatta acaggtcaat ctttttaata tcatcctttt tagtaatctt atatattccg
     3601 cttgacataa caattttata cttagatgag ttagtagtat ttaagttaca aatttcttta
     3661 tttattttag ataatctatt taaaagttca tcttcagtat tacaagcaat caacaatata
     3721 aaatggtcat tatccatcct agcacaaatt tcatatttat ttgtattatt atttaaaacc
     3781 tgtgatatat gaattaatat cttattccct tcagcatatc caaacatctc atttatatat
     3841 ttaaatttat tgacatccaa ttgtactaag taaaaattat cttgataata attctttaaa
     3901 atatacatag aatcttttct aaacttttct ttatttttta ttccacttaa agaatcataa
     3961 taagccaatt ttttaagttg ttctattaat ttagcatta
//
LOCUS       DAEPOZ010000279         3968 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.279, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000279 DAEPOZ010000000
VERSION     DAEPOZ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.279"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(247..627)
                     /locus_tag="KR440_003364"
     CDS             complement(247..627)
                     /locus_tag="KR440_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH0700367.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            complement(900..2258)
                     /locus_tag="KR440_003365"
     CDS             complement(900..2258)
                     /locus_tag="KR440_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0700368.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTIMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDSEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            2706..3158
                     /locus_tag="KR440_003366"
     CDS             2706..3158
                     /locus_tag="KR440_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HBH0700369.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            complement(3315..3932)
                     /locus_tag="KR440_003367"
     CDS             complement(3315..3932)
                     /locus_tag="KR440_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBH0700370.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFIYIYMGKEFGAKRNNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDREQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
BASE COUNT         1318 a          694 c          375 g         1581 t
ORIGIN      
        1 ttttttatct ttacctcagc tacaaaggca tcattacctt caaccatttc taatagttca
       61 tacgcttttt tacgacctat tttagttgct atatctttta gtactgccat tttcttaaac
      121 attttatcac ctctactcaa aatttacact taatttttaa ttatataatt ctaatcctag
      181 actagtttat aataattatt gtctctaatt atatcagata ttgagtaaat ttaatacttt
      241 gtaaaattat ttatcctctc tgactaaata atcatttcca tcttggtctt taaaagaaaa
      301 catccttcca tatggcatct ccattaattc cccaacttca acctcgttat ttgccatttg
      361 ttcataagtt ccattaatat ctcttgtact taaaattatt gatggatgag atacatttat
      421 ctctttgttt tgcttcatca ttagattttt atcatacaac acaaatgttg taaattcatt
      481 tttacttggt gctacttcca cccatttcat atttggaccc atttcctttt cataagttac
      541 tacaaagttc aatttttctg tccaaaacaa ctttgcatcc tcttgattat ttacatagag
      601 agttattttt cctattttat ttatcataac aatacccacc tttattatta aataaatacc
      661 taatattttt tatatttaat aatataccca tatatattat tttctataga tatatatttc
      721 taatatttat cgccaaatta tgtaattctg ctcaatatat aatttataat atccatacta
      781 ttatagcctt tatttatgta atttaataac cttatatatc atacttataa ttatgataat
      841 aactacataa aaaatcactt ctaaatagag ttatccatct aaaagtgatt tcatatattc
      901 tatgcaactt tgtgttttag ccactttgtt cctcttaatc ttaaacaata taaagtccct
      961 ctaacaagcc aatcagtata catacctatc caaattccta acactccaaa ttctaataca
     1021 acccccaata cgtaacctgt aaaaattcta aaaatccaca tacctattac tgcagtcatc
     1081 attgtatatc tagtatcacc ggctcccttt agccctgctg ataatacaaa ggatataggc
     1141 catattacca tagctaaaca atcacttcga attaattgtg ttgagatgtc aattacacta
     1201 gcattttgag tatacatact tgaaatccag ggtgcaattg gtataaatat agctcctact
     1261 ataaataaac aaattgttgc aaatttagtt aaatataata aagtactctt agctccttta
     1321 atatctccac gtcctatata ctgtcccacc aaagttgtag cagacaaagc taaagcattc
     1381 ccaattacat ttattatgga tgcaattgac attcctattg cattggatgc aattgctgat
     1441 gttcccatag taactataaa tacttgaaca atcaatttac ctgcattaaa aagtagttgt
     1501 tcaactccag ctggtatccc tatattaaat atagactttt gagtttcaac atcaatctta
     1561 aatggaaata ttttacccat agatataaat tcatttcctt taaataaagc tcttataatt
     1621 actatacatc caattattct agctatagca atagatatag ctgcacctgt tatgccatag
     1681 gatggtgttt gcaaatttat tcctagagga tttaaatcaa taccatatat aagtatatat
     1741 cctaatgtta catttactat attcataaac atagtcacat acataggttt ctttacatct
     1801 ccacaccctc ttaaaatacc atttgctatt tgttgtgtcg caataaatgg atacgtaaat
     1861 aatgtaaact ctatgtataa ttttgcattt gattttacca attcttctgc tgaaccataa
     1921 aataaattaa ttacaccagt tctaaaaacc cacatcaata ttgttattgt aaaggataat
     1981 attactccag acactagagc ctgcttaata gtttcattca ccttttcact atcttttttt
     2041 cctatttgtt gagctacaac gacagtagct ccaacagata atgcagcaaa tacagatata
     2101 aatatattgt ttattgaatc aaccattcca actgcagata cagcttcttc cccaatatga
     2161 cctgccatta ttgtattaaa tactcctaaa agcatgacaa acgtctgctc tatcattata
     2221 ggaattgcta actttataac atttttcctt ataaacatcc ctattcctct tttccctctt
     2281 tatataaatt taatttttaa ttagtttatc gaactgtaaa ttgacaatgc aaagtttaga
     2341 attgtattgt tcgcattata atatactgct ttcaaataat ataaatcttg tttaatatca
     2401 tttgaacaca gtatatcttt ataattatct tatgtttatt attttttatg gataccacat
     2461 ttaaaatatt tattaattta atataaatat tttccgttat tattaaaagt agctatattt
     2521 cgttaatatt gtatcactat cgtgtagcaa taacaataag tacattttat atatttttta
     2581 taatattatt taatcatcca aacttttacc actttttcaa tatataaaat attacacttt
     2641 agatgtaaca aaatcgcatc catttaaata ttgtatttta tagactaatt ttaggatgtg
     2701 atactttggt taatttaaat gcaaataatc taaatgtaaa tatggtttat cctatttctt
     2761 ccacagcacc catacgtttc agaagatata ctgtttcata tttagaaaac atggaaaata
     2821 tttatttaac tatagcaaca tactttgata cacttggttt aggcacaatg aatatcgaca
     2881 aagtttatgg tcaatgggct tggtttactg ataatatata tgatttaaat ctatttgttt
     2941 ttgtaggaaa ttatccttat caaattgcta aaaacagata tgatacattt gtagatattt
     3001 tgccagtagc tattacttct attgtcaatg gagatagagt atttttaatt tcaaatcctt
     3061 gtttgttaaa ttcaaaaatt acagttagat ttatatctag tcatcctact cttaatagaa
     3121 cagttgatta tggcaatatt agagatttta ttttttaaaa ctgcaacatg atgtgctatt
     3181 aaaaaatacc ctaaatttaa tgatattatt aaataactag aatattttta attggcacat
     3241 catgcctagc tattataaat tcataatatt attttactta agataagtac tgtattttat
     3301 ctacttgtat atttttattt acctagtttc tcagttctat atcctatttc tttattagct
     3361 agcttataac cttcttgtat catttcttct ctcgttaatg cactccctaa taaattgtca
     3421 atactcagtt gctctctatc actaaaatac ttatccaata atgattcctc tatatcttcc
     3481 tgatatgcca aatttccttc atgcattata tattttacat cgaaaccttc tttatttaat
     3541 acttttccca ccaaaatact tctatgacat cttataggtt cttgcatagc tcctaataaa
     3601 actatcctat aacccatttg acaacccttt tttaatcttt ctattccgtt taaaaaaata
     3661 tcttcttttg ctactttctc aaaatctgca taaccttctt gcgtatatac atctttatta
     3721 tttcttttag ctccaaattc ttttcccatg tatatatata taaatccgga atttctaaga
     3781 gattcgttaa atgcctcttt attgtattga atattatatt tagaatatgg agtttctcta
     3841 atgtcaacta cacaatttat gtcatatttt tttatcatat taataagctt atcatatgga
     3901 taatttgaat gtcctattgc gaatatttcc atataaatac cctcctattc tttgaaatta
     3961 gtatctat
//
LOCUS       DAEPOZ010000280         3939 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.280, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000280 DAEPOZ010000000
VERSION     DAEPOZ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.280"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..239
                     /locus_tag="KR440_003368"
     CDS             <1..239
                     /locus_tag="KR440_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005209661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700371.1"
                     /translation="VEESRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKIRRTAIEF
                     STEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            497..1546
                     /gene="carA"
                     /locus_tag="KR440_003369"
     CDS             497..1546
                     /gene="carA"
                     /locus_tag="KR440_003369"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBH0700372.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            1739..>3939
                     /gene="carB"
                     /locus_tag="KR440_003370"
     CDS             1739..>3939
                     /gene="carB"
                     /locus_tag="KR440_003370"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HBH0700373.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYL"
BASE COUNT         1630 a          400 c          777 g         1132 t
ORIGIN      
        1 gagtagaaga atctagacca aacatattag atgctataag aaataaacaa gttgatatag
       61 taataaatac accaactaaa ggaaatgact ctacaagaga tggatttaag ataagaagaa
      121 cagctattga attttctact gaaataatga catctttgga tacactaaaa gctttagtag
      181 aagttaagaa gaaacattta aataaagatg agttaaaagt ttacaatata gctgaataga
      241 aattaagttg attgtatagt ttaagaattt aattattaaa taaaaaaaaa gaggttattt
      301 tttctaggag tttttacttt tgagataact tctttttttt aagaaatttg tagtataata
      361 gaaaaagata aaaaataaat gtaaataagt ataaaactag aatgtaaaag tgaaaaatag
      421 atatagatat ttaaaagctt atatttagtt aatgttcttt agtttgtcta aagagaatta
      481 taggaggtac tgtagtatga aagcaaggtt aatattagaa gatggaacag tttttatagg
      541 aaaggcattt ggatatttag aagaaagtgt aggagaagta gtatttaata cttcgatgat
      601 aggatatggt gaagttttaa cagacccttc ttactatggt caaatagtta ctatgactta
      661 tcctcttgta ggaaattatg gtataaattt atcatcagca gagtctgaaa aagttcaagt
      721 aaagggattc atagtaaggg agaaaagtga ttctccaagt aattttagat gtgaaattga
      781 tatagaccaa tatttaaagc aaaataaagt tatcggactt gaaggaatag atacaagagc
      841 tttgactaag atacttagaa ataatggaac aatgaaaggt ataattacat tagaagacag
      901 taaattagaa gatgttaaac ataaattgga taagttttca aatacagaag cagttagaac
      961 tgttacaaga aaagaagtag agcatataaa aggaaatggt ccaaaagtgg ctgttatgga
     1021 ttttggagta aaaagaaata tattaagatc atttatagct cgtggatgtg atataacaat
     1081 atttccagct acaacatctc cagaagatgt attgagtata aaccctgact taatattctt
     1141 gtcaaatgga cctggagacc cagaagacct agaagatgtt atagaaaata taaaagctct
     1201 tattggtaaa aaacctatag ttggaatatg cttaggacat caacttttag ctttagctct
     1261 tggaggaaaa actgctaaac ttaaatttgg gcatagaggt ggaaatcatc cagttaaaga
     1321 tttagaagaa ggaaaagtat ttataacttc tcaaaatcat ggttattatg tttcagaagt
     1381 tcctgaacaa atgaaagtga cacatataaa tttaaatgat aatactgttg aaggaatgag
     1441 acatgagaag ctagatgtat acagtgttca ataccatcct gaagcttgcc caggaccaaa
     1501 agataatgac tatatttttg ataaattttt agaattagta aaataaataa cattagaata
     1561 gctaactaaa aaacaagaat aacttaaatc aaaaagtttg gtttaattaa catagatgta
     1621 ttaaatagat gtattgatga gtaaatttga acttaagata aaataaagta attaaagttg
     1681 attgtataga tgatgaagat aagaactgtg aaaattgttt agataaggag aaagagttat
     1741 gcctaaatta gatagtataa agaaaacttt agtattaggg tcagggccaa taataatagg
     1801 tcaagctgca gagtttgact attcaggaac acaggcctgc caagcattaa aagaagaggg
     1861 aattgaagtt gtattagtaa atagtaaccc agctactata atgactgata aagaaattgc
     1921 agataaaata tatatagaac cattaacaat agaatttata gaaaaaataa tagaaaaaga
     1981 aagaccagac agtttacttg caggtatggg tggtcagaca ggattaaact tagctgtaga
     2041 attacatgat gctggtatat tagataaata taatgtaaaa gttataggaa catctataga
     2101 atctataaaa aagggcgaag atagagattt atttagagaa gtaatgaaag aaataaatca
     2161 accagtaata gttagtgata tagttacaaa tttagaagca ggtcttgagt ttgccaataa
     2221 gataggatat ccagtagttg taagaccagc atatacgcta ggtggaactg gtggaggtat
     2281 agctgatact gaggaagaat taagagaaat actttcacat ggactacagc taagtcctgt
     2341 tggacaagtt cttcttgaaa agagtataaa aggttggaaa gaaatagaat atgaagtaat
     2401 gagagatgga aatggaaact gtataactgt atgtaatatg gaaaatgtag acccagttgg
     2461 agttcataca ggagacagta tagttgttgc tccaagtcaa actctaagtg atgaagagta
     2521 tcaactactt agaaaggcat caatagacat aataaatgca atagaggttc aaggtggatg
     2581 taacgttcaa atagctttaa acccacatag tttagaatat gcgataatag aaataaaccc
     2641 aagagttagt aggtcatcag ccctagcatc aaaagctaca ggatacccaa tagcaaaagt
     2701 agcagctaag atagctttag gttatacttt ggatgagata gaaaatgcag taactaaaaa
     2761 aacttatgct tgttttgagc caacattaga ttatgtggta gtaaaaatac caaagtggcc
     2821 atttgataaa tttagacatg caaatagaaa attaggaact aagatgatgg caactggtga
     2881 aatcatgagt ataggaagta actttgaggc tgctattctt aaagggataa gatcacttga
     2941 aacaggaaaa tattcattgg ttcatgctcc atcagaaggt agaacattag aagaattaaa
     3001 ggctagagta gtagtacctg atgatgagag actatttgac ttagctgaaa tgataagaag
     3061 aggctataaa gtagaaatga tatcagaaat aactggtgtt gataagtggt ttataaataa
     3121 atttaaatgg atagtagaac aagaagaaaa gttaaaagtt ctaaaaatag aagacttaga
     3181 taaagaatat ttacatgaat taaagaaaaa gggattttct gataaaggta tatctgactt
     3241 aatgaagata agcccagaaa aattatatga attgagaagt ttatataata tacaaccagt
     3301 ttataaaatg gttgatacat gtggaggaga atttgaagct ctatcacctt attactattc
     3361 aacttatgaa caatatgatg aggtagtagt tagtgataag agaaaagttg tagtattggg
     3421 ttctggtcca ataagaatag gtcaaggtat agaatttgac tattgttcag ttcactgtgt
     3481 aaaatcttta agaaaaatgg gaatagaaac tataattgta aacaataacc ctgaaactgt
     3541 aagtacggat tttgatacat ctgataagtt gtattttgaa ccattaacag aagaagaagt
     3601 tttaaatata atagaaaaag aaaaaccaga aggtgtaata ctacagtttg gtggacaaac
     3661 agctataaaa ttggctaagt ttttacatga aaaaaatata cctatactgg gaacagactt
     3721 tagagatata gatgcagcag aagatagaga aaaatttgat gatttactag agagactaga
     3781 tataaataga ccaaaaggaa aaggtgtatg gacaactaat gaaggtgttg aaattgctaa
     3841 ggagttaggt tatccagtat tagttagacc atcttatgta cttggtggtc aaggtatgga
     3901 aataacttat aacgaagaaa aactaattca gtatttgga
//
LOCUS       DAEPOZ010000281         3911 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.281, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000281 DAEPOZ010000000
VERSION     DAEPOZ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.281"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            894..1646
                     /locus_tag="KR440_003371"
     CDS             894..1646
                     /locus_tag="KR440_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0700374.1"
                     /translation="MLKVNQLCKSYKTGNKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            1636..3747
                     /locus_tag="KR440_003372"
     CDS             1636..3747
                     /locus_tag="KR440_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0700375.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYI
                     VPSLTINKLNYRLPSVWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVAGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIERVYMKKSSYDMFFNSK
                     DFSKLIDKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNDEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
BASE COUNT         1463 a          557 c          498 g         1393 t
ORIGIN      
        1 tttattacag ttctttgcta tactacagac ctttattatt gcaacatttc tattacattg
       61 caaagtctta tttattacag ttctttatta tactacagac ttttattatt acaacatttc
      121 tattatatta caatctctta tttattacag ttctttatta tactacagac ttttattatt
      181 acagcatttc tattacattg caaagtctta tttattacag ttctttatta tactacaaac
      241 ctttattatt acaacatttt attattttaa cgtctttact ataatatagt aatattttaa
      301 tatctcaaaa tcatatcaat cacttttcag catagtctaa aatacttcca ctttctaaaa
      361 ttatctatca tacctatact aataatactt tttaaccttt ttattataca tttcaattgt
      421 ttttaacgac ctatttattt ccaaaactcc aatatatttt ctcaattaca ttgtgttaat
      481 aacccctaaa tcctttcaaa ttatttacaa tacattccat taaataatct tactgctcta
      541 taattctttt tttcttttta accctactat acattattta catcattttt ttcaatacaa
      601 cattcgttat tctataacct ttttactttt actatatacc ttttttctaa ctacaccttc
      661 aatgttccac atcatattac tttagcacaa caattaatac ctaaaaataa ccctctaaat
      721 cctccaataa tataatcaat ttaactataa taataacctc tcacactatc caaacaatat
      781 attttcaaac attacaaaaa aacatccaac tattacaata ttgctcatat ccctcctccc
      841 ctttcaaatg ctatcattga cttacaagat aaatgaaagt gaggatgata tatatgctta
      901 aagtaaacca attatgtaaa tcatataaaa caggcaataa aacatatcct gtattaaaaa
      961 atatttcttt tgaaatcaac aaaggagagt ttgtcgcaat catgggttca tcaggaagtg
     1021 gaaaaactac tcttttaaat tgtatttcat gttttatccc aattgataat ggggaaataa
     1081 ttcttggaaa taataatata agaaatttaa aagaaaagga actttcagaa gttagaaacc
     1141 aacaactagg atttgtattc caagatttca tgctatttga tggtcttagt gtctttgaaa
     1201 atatatgtgt tccacaaatt atcaaaggta gtcaagtctt acaaatggaa aaccgtgcaa
     1261 ttaatctatg cgagctattt ggtattgaac atatcagaaa aaaataccct gccgaaatat
     1321 ctggtggaga aaaacagcgt acagcagtag caagagcact catgaacaac ccagatttaa
     1381 tattagcaga tgaaccaact ggtaaccttg atagtaagtc ttgtcgtaca gtcattgaat
     1441 catttttaaa agcaaagcaa gaattagatg ctactatact catggttaca cacgatagct
     1501 ttgcagcaag cttctgcgac cgagttattg tgcttaaaga tggaataatt tataaagaag
     1561 ttgtaaaatc aaattcaaga cataaattcc tagatgaatt actagaagtt ttaaaagaaa
     1621 tgggaggcgc tcaagatgac aattaataaa ttatacagca aacttcgagc ctataattgg
     1681 aagaactata caactttatt cttttgtata atactttctg tggtattagt aacttcatat
     1741 gctctaattt attttagccc aacaatacaa aacatacttc caactggtgg agatagcaga
     1801 aaacaggcta caatgatatt ctcagttgct atattaggat gcgctgtctt tactacatac
     1861 gcttcttccc tctttttaaa atttaaaagc aaggaaattg gtattttcat ggctctagga
     1921 acagataaga agaaattaaa aaaacttcta ttttcagaga tattgttaat ttcaatagta
     1981 tgctgttttt taggtcttat attaagtatt cctgtatctt ttggtatatg gaaaatattt
     2041 caattagtga ttattgatac cagagaaatg acatatcatt ttggaactca aggattcata
     2101 tatggattta tatttagtgt atttgtgaca ttatgcatat ttatactttc tgtaaaattt
     2161 attaatcgta caaatataat tgatatatta aatgaccaga gaatatctga accaatacag
     2221 gatgtaaagg actggtatgg tatattaggt ataattttta ttatactagg tatttttcta
     2281 ggatatatcg taccttcact aactataaac aaattaaatt atagactacc atctgtatgg
     2341 agctttactt atctactctc tcttattgga atatatatga ttatgacata catagttgta
     2401 catgctaaaa gaggaaaaca tccagaaaaa tactataaaa acatcatatc aactagcatg
     2461 atgagattta tgggaagaca aacagtaaaa aacatgtgtg ttatttcatt tttgattgca
     2521 ggagcattat ttgctgtatt ctatgttcct aatatggttg caggtatcta tgatactctt
     2581 aaaaataacc ctatagatta tacttttacc tacaatcaaa gagataaaca ggtatcaaaa
     2641 aaagatattg aacaattagc agataaacac aatataaaaa ttcaaaacta tatggaaatt
     2701 ccttctatag aattaattgt tgatggtgta gaatcaatat atcataaaga tggaactatc
     2761 gaaagggtat atatgaaaaa aagctcctat gatatgttct ttaattctaa agatttttcc
     2821 aagctaattg acaaaccaat aactataaaa aatggtgaat atcttgctgt tgtaaataaa
     2881 ggaaatgaaa atcaagatta taaatatacc aaatttatat caagcccagt cacagaaaaa
     2941 gaaatcccct ttagatatgc tggaaaaata atatttaatg gttctttttt tcaaaataga
     3001 agtatgaacg cctatgtcct taatgatgag gattataaat catttactaa agatttatcc
     3061 ataaaaaatt acaataattc tatactattt aatgtagata aagataccta tgcttttgcc
     3121 aatgacttaa agaaagaaat tataaaaaga acatctaagg aaatgggcat gttcagtggt
     3181 tatgatgaat acgtaaaaaa acgttatgaa tcaactggaa gaaaatattt tatggatgaa
     3241 gaatatccaa gtggtgaagg tcatttaaaa ctagacccaa acaacaatca gttgtatctt
     3301 aattggaaat actatcctaa ctttaaaatt ttacaaagcc aagatatgat aaaaaacatg
     3361 gcagtcttct taatgttatt catatatgtg gcaatcatat gttttacagc agtaggaatc
     3421 attgcatata caaggggtgt tactatagct ataaatcaca agcaggtctt tatggattta
     3481 aatcgattag gagcaaacaa tgattatata aagttctgta ttagaaatca attaaagaaa
     3541 gtattttcat accctcttat tgcaggaagt tttttaattt atctgtttaa ttttatgatt
     3601 atgttaaata atgatggtag aatcgtcaat agtgaggtca atgctctttt gataaactta
     3661 ggttttatag ggatttgtgg agtttatatg taccttatat atctattaac ttataagaaa
     3721 ttcaaaaaaa ttattggtat tttataataa ggaaggttgt tagattttta aagtaataaa
     3781 atcattactt tttataataa gaaaagcatt agcattttaa attaaaaaag gttattagat
     3841 ttttatgata tgaaaagtca ttgtattttt atagtaagat aattatttgc tttttataat
     3901 atgaaaatcc a
//
LOCUS       DAEPOZ010000282         3909 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.282, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000282 DAEPOZ010000000
VERSION     DAEPOZ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.282"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            75..374
                     /locus_tag="KR440_003373"
     CDS             75..374
                     /locus_tag="KR440_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700376.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
     gene            698..2353
                     /gene="ilvD"
                     /locus_tag="KR440_003374"
     CDS             698..2353
                     /gene="ilvD"
                     /locus_tag="KR440_003374"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBH0700377.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEELAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIEFNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            2697..3635
                     /locus_tag="KR440_003375"
     CDS             2697..3635
                     /locus_tag="KR440_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBH0700378.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNSKR"
BASE COUNT         1549 a          400 c          733 g         1227 t
ORIGIN      
        1 ttttatcgta tgtataaata ttataaatat atggaaataa taatagtata ttttggtaaa
       61 gtaggaggaa ttttatgaaa tggattaaat ggtatagtat tacatgcata tgtattttta
      121 tagtagtagc tttttatatg tttatatttc ctaataaaat agaaactatt gatacttcat
      181 ctgcatatag ctttgttgaa aagaaagtac caaatagtgc tgtatatcaa ggatacaaaa
      241 agaatccagt tgatggtact actacaattt attacagcta tgataattca actcatatag
      301 ttagattatc acatccagaa gactattcaa ggaagattaa ttgggataaa gtttcaaaca
      361 tcagatttga ttaatcttta atatttataa aagtctatat atctaaggca aatatattat
      421 taatcttatt catagttgag cactttctat tatagaagtg cttcttctga tttatatcat
      481 aaaatattac tcaacaattt gtaaaatata ttagtttata aaatatattg aaaagataat
      541 attatataat acgattgtgt aagatattga taaaaaatcg taataaattt tcattagtat
      601 atgctaatat ttttttacaa ttttcgtaat taattaatag aaaaatatat tataataata
      661 ttatgaaaat ttaaaaaagt aagaaagagg taatattatg agaagtgata taaaaaaagg
      721 aatagaagga gctccaaaga gagcgttaat gtatggaatg ggtcttacta aagaagaaat
      781 tgaaagacca ttaataggta tagtaaatgc acaaaatgag gtgattcctg gtcatctgca
      841 tcttgatgaa atagcagaag ctgcaaaaaa tggagtaaga atgagtggtg gtttgccatt
      901 agaatttcct gctatagggg tatgtgatgg tatagctatg gggcatgtcg gaatgaatta
      961 ttccctggct tcaagagaat taatagcaga ttcaatagaa gctatggcta tggcacatgg
     1021 ttttgatgct ttggtactta tacctaattg tgacaagata gtgccaggta tgttaatggc
     1081 tgctgcaaga cttaatatac caagtattgt tgtgagtgga gggcctatgc ttccaggtaa
     1141 aaaaaatgga aaagtatatg attttaactc tgcaatggag ggtgttggag catgtaaaga
     1201 tggaacagta agtgaagaag aattagaaga gttagccatg aactcttgtc ctggatgtgg
     1261 ttcatgctct ggacttttca cagcaaatag catgaattgt ttaacagaag ctttaggcat
     1321 gggtatacca tataatggta ctgctgcgtc acattctgga gaaagaaaaa gaatagcaaa
     1381 atatgcaggt atgtatgtta tggagttact taagaacgac ataaaaccta gagatatttt
     1441 aacaatagat gcttttaaaa atgctatagc tgttgatatg gcaatggctg gttctacaaa
     1501 tacagtactt cacttacctg caatagctta tgaatcagga atagagttta acttagattt
     1561 ttttgatgaa ataagtgaaa aaactccttg tttaacaaaa ttaagtccaa gtggaaaaca
     1621 tcatattgaa gatttacata tggcaggagg aataccagct ataatgaacg aactttcaaa
     1681 gataaatgga ataaatttag attgcaaaac cgtaacaggc aagactatag gggaaaatat
     1741 aagaaattgt gaaatagaaa atgaagaagt aatacataca ttaaagaatc catatagtaa
     1801 ccaaggtggg cttgcaatat tgaaaggaaa tcttgctcta aatggagctg ttgtaaaaaa
     1861 gtcagcagtt gcagaagaaa tgttagttca tgaaggacca gcaagagttt ttaattcaga
     1921 agaagaagct gtaaatgcta tttttggtaa aaaaataaac aaaggtgatg ttatagttat
     1981 aagatatgaa ggtccaaagg gtggtccagg aatgaaagaa atgctatctc ctacatcagc
     2041 agttgcagga atgggacttg ataagcatgt agcacttctt actgatggtc gtttttcagg
     2101 agcaactaga ggagcatcta taggccatat ttctccagaa gctatggaag gtggtttaat
     2161 cggactagtt gaagaaggag atataatttc tataaacata ccagacaaaa aattagagtt
     2221 aaaagtagat gaagttgaaa tagaaaatag aaaattaaaa tttaaacctt tagaaccgaa
     2281 aataaagcat ggatacttaa gtagatatgc taaattggta acatcagcaa atacaggagc
     2341 agttttaaaa tagtgaacta tatttacgaa aattagaaaa aattaagaaa atattaattg
     2401 gatatcttag gtgttatagc actaatgagg tatccttttt attttgtatg aaaaatatac
     2461 tgtttgtata caaaaatatt aaagatattt tagtaagttt tgtatataag caaacatgta
     2521 ttttttaata tagttatcta aatattattt tatatacaaa aaaatatgac tcctaaaaat
     2581 aaaaaatata tcataaatat aaataagtag atataggttt taatttgatt tataaaccta
     2641 ttataaaata aaattcatga tttttataag caataaaatt tttaggagga tatatgatga
     2701 ataaaagaat gaaactaatt ccgtatgaaa taaatgaaaa tctaagaggt gcaaaaaata
     2761 aattcccata tggaataaaa caaatgaatg ctaggggaat gtgggatgaa ggttatactg
     2821 gtaaaaatat tgtagttggt ataatagata caggttgtga tatatctcat cctcttttaa
     2881 aaggaaaaat aattggtggt gcaaatttta gtgatgacag taatggaaat aaaaatatat
     2941 atgaggattt taatggccat ggaactcatg tggcgggtat tatagctgca tctaactata
     3001 ataatgaagt tatgggagta gctccagatt gtaaattatt aatagcaaaa gcattaaata
     3061 aagatggtac cggaacatat caaagtataa ttaatgctat taactttgct gtaaataaca
     3121 aggttgatat tatatctatg tctcttgggg gaaacaaaga tgataagaat ttaaaaaatg
     3181 ctgtcatgca agcagtaaaa aataatattt ctgtagtgtg tgcagcaggt aataatggag
     3241 atggtgattc tagtacaagt gagtatagtt atccagccag ttatgctgag gtaatagaag
     3301 taggtgcaat aaatgaaaac tatttggttg aaaagtttag taattcaaat actacaatag
     3361 atttggtggc tccaggaaga aatattatat cgacttatat ggataataaa cttgctatta
     3421 tgagtggtac tagtatgagt gcaccatacg tatcaggctc attagcacta attaaagaat
     3481 gggcaagaga ggagtttgaa agagatttag atgaagctga actgtatgca caattaataa
     3541 aatgtacgag agcgcttgga atacctagaa cggaacaagg aaatggatac ttatatttaa
     3601 atctttataa atacaagaat aatagcaaaa gataattttt gatttgatga taaaatagct
     3661 atattatata gagtcgagac aaataataaa aattacttag gtgtaagatt ttacatcaat
     3721 gtataaaggt attaagtgat aaaatttata aacatattag ctagttagaa ttgaaaaata
     3781 aatatacgat attatagata gcacatctgg aaaaggtgtg tttttctatg tacttataca
     3841 tataaaagat ttataagaga tgcaaaagta ctataagtta gaaatttttc tatgaaagat
     3901 tagaaatac
//
LOCUS       DAEPOZ010000283         3900 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.283, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000283 DAEPOZ010000000
VERSION     DAEPOZ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.283"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..210)
                     /locus_tag="KR440_003376"
     CDS             complement(<1..210)
                     /locus_tag="KR440_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0700379.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAEIDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVH"
     gene            complement(388..1281)
                     /locus_tag="KR440_003377"
     CDS             complement(388..1281)
                     /locus_tag="KR440_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBH0700380.1"
                     /translation="MQLYNTPFNTNKHLIYFFTSTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIRIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            complement(1538..1975)
                     /locus_tag="KR440_003378"
     CDS             complement(1538..1975)
                     /locus_tag="KR440_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HBH0700381.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSTPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
     gene            complement(2232..2381)
                     /locus_tag="KR440_003379"
     CDS             complement(2232..2381)
                     /locus_tag="KR440_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HBH0700382.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            complement(2394..2624)
                     /locus_tag="KR440_003380"
     CDS             complement(2394..2624)
                     /locus_tag="KR440_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HBH0700383.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            complement(3316..3537)
                     /locus_tag="KR440_003381"
     CDS             complement(3316..3537)
                     /locus_tag="KR440_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HBH0700384.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
BASE COUNT         1363 a          465 c          482 g         1590 t
ORIGIN      
        1 atgtacgata tttgctattt ctgcaacgct aaattttgaa tctctcaata atacaattct
       61 ttgtaatatt gaaatctgtt caactgagta taatctatga cctgtatatt tgtctatttc
      121 tgcaggcttt aaaataccaa gttcatcata ataacgcaac atacgaatag aaatttgtgt
      181 tagttttgaa acctctccta ttttaaacat gaaaatatga cctccttttc acttataatt
      241 agtatatatt ttgtttttat atacttcaag cgattgtgga aatacaatta ttttccagat
      301 gttgcaataa attatagaga gtaaattata tttaagtagt aatcgtatca aatcatagat
      361 tatcttaatt tataattcat attaaaatta ccagtctaac tcaaattcaa aatcatccga
      421 ctttatctgt tcaatttcag aatttacctt ctgtttaaat ttattagaag aaatcatata
      481 tacaagaaat attaaaccaa atattatttg tacatattga gctattacac ataatttaat
      541 agggtagtgt agaagtaatt gaatgttgaa aaaatagtac atcatatgaa ctaacccagc
      601 tgataaaatg cttctacttg tattgatata tacaaaatta attataaagt ttaaaagtac
      661 aatgtttggt ataaataaaa aagcatcaaa tagggagtat tgtgccaaat tatatggtat
      721 ttgattaggt gtaaaatacg accctagata ccagattcca caaacaagcc ctaaaataat
      781 tgaagagcct ataaatccaa atctaactaa taatttgtca atggcgtatc ctctccatcc
      841 agcttcttga ctaagtatac tcaatataaa acttgctaat agcatataag ctattttaga
      901 aggtctaaac actgctatgt gtatccaatc tatatcagaa gtttttacat taaagtaatt
      961 tccgattatt aacgaagtaa taaaagttaa agcaaaaaat aatattgtta atagtatcca
     1021 ttttattcta atttctttaa agcttaggta tctataaata aaatcttgtt ttgcactttg
     1081 tggatagatt gtagaaataa aaaatagacc tacaatagct ggtgatgtat atgcaagata
     1141 aaaaatgatt tctccaagta ttgtatttgt aattccgagc aacgcaggtg taagactgaa
     1201 tatccagttc aatactagcg tagatgtaaa gaaatagatt aagtgtttgt ttgtattaaa
     1261 gggggtgtta tatagttgca ttttttcact atccttccgt tttataatga agttttaatt
     1321 tttattaatt gtttatttat ttttatatgt attaagttat cgtttgatgt tagttattgc
     1381 ctataataat atcctatttt ttatattttg attaattatt tttacactaa atataattaa
     1441 ttagtattta atattgaaca tattatatta ttattaatta ttaaataggt caataaattt
     1501 gcaattagtg atattaataa attactttag atttttatta aatgtttagc ttttctttaa
     1561 tattttttat ataacgacga tttataataa ctttttcgtt gttttttaaa gtagcttgca
     1621 tgcgattatg tgatattact tttatacttt ttatgtagtc catatttaaa atcatggatt
     1681 tagatattcg tgagaaggat gtattagcta ataattttga taatttatat atttttaaat
     1741 caatttcaaa tacatcatct tcataataag caaatgtttt atttccaact gtttcaaagt
     1801 ataaaatatc acagacattg agtttataaa tctctcctga ttttttacct tttatatcaa
     1861 gtaatggtgt actcaataat gaaattattt tttcaatgtt attatctaga gcagaacatt
     1921 ttatggttat ttcaatatca tcacaattaa taccttgttc gacatgtatt ttcattaatc
     1981 atcacctcat tttattactg tcattagaat agcattatat cttattgagt ccaactagat
     2041 gcgttctaaa tttcaaatta agcatagtga aattcaaaaa taaaatagtg aaattcaaat
     2101 ttgaggtggt aaaattcaaa aaaatactat ggatatttgg ataaattatc caaatatcct
     2161 agtatttagc atagaatatg tctaattatt ttacaaatag acctatttta tgacttacat
     2221 atattgtcaa attacttctt aaatatcaaa atatttttgg ataatgtatc aatactgttt
     2281 gataaagtat ttaatttacc ctcaattcta accaacaagt acatggatag agctatagga
     2341 aaaccaacat tggttattaa cagttgtaaa ctatcatcca taaaattagc cccctattct
     2401 ataactaagt cataaggagt ggtatttgtt tccacaacct tggcttcaac aacagaagct
     2461 aggtcaaatc ctccaaccga aaaaatattt ttagatacta tcaaatccat gacagatttt
     2521 atattttctt cagttaaatc atcctttgga ttttctatag aaaaagaaac ttgtttatcc
     2581 tttacatttt taaagctcat gactaatttt ttcttaattt ccatataatt acctccgata
     2641 taataattta tattttaatt gtttgcttgt taatattggt attttaattg tcaatattag
     2701 tgttttgctt gttagtatta atatttcaat tgtcaatatt aatgttttac ttgttagtat
     2761 tagtatttca attgtcaata ttagtgtttt gcttgttagt attaatattt caattgtcaa
     2821 tattagtgtt ttgcttgtta gtattaatat ttcaattgtc aatattagtg ttttgcttgt
     2881 tagtattagt atttcaattg tcaatattag tgttttgctt gttagtatta gtatttcaat
     2941 tgtcaatatt agtgttttgt ttgttagtat cagtgtttca attgtcaata ttagtgtttt
     3001 gtttgttagt atcagtgttt caattgtcaa tattagtatt ttaattgttg actctagtta
     3061 ttgctgatat agaaaatatt taatttattt caattaatta gattaaagta gggtagcatt
     3121 taaaatagct aacatgttta agttgaaata cgtttaatat attaattttt caaagcatta
     3181 atatattaaa gttataacca aaattgaagt tataatctgc caaatctata gcgtattaag
     3241 tgcgttatat aacttaatct tttatataaa gagttaattt ttttaatttg taaagcagag
     3301 tacgtttaaa agatactatt tagataatga tgtactatct aattttaaaa tatctacaat
     3361 agggttctct tgaagacttg ctattgcttc tgcaacagca taaacatctt catttaatgc
     3421 ctctgctttt acatttgcaa atgatttact tttaacaact gccttaccct tatcatcaaa
     3481 accagcatcc aattttagtt ttatactaga taaatttttt gttgaaacaa cttccataaa
     3541 attacctcct aaaaatttaa tttcaaagat ttgtcgactt tctttgttag ctataatact
     3601 atcatctaag ataatatcgg agttaaacag taatttctaa agattttttg tgtaaatttt
     3661 tataggaaaa attttacttt tctgatgatg aaaaagttta caagttattt ttgaagatat
     3721 gttcttagtg ttttgaaata gtatttgtat atattcatgc ttagttgaga tagttttgta
     3781 tggattttca tatgtgtaga gttaaatgtt aggtaccaaa gtttatgtta aattaaatgt
     3841 taaagcttat gttaaaaatt ttattaaatt taatctgtaa ataaaaaagt taaaagtcca
//
LOCUS       DAEPOZ010000284         3830 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.285, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000284 DAEPOZ010000000
VERSION     DAEPOZ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.285"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..1241
                     /locus_tag="KR440_003382"
     CDS             <1..1241
                     /locus_tag="KR440_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0700385.1"
                     /translation="LENENIKKIFSLSKAHDEVIKFNNKYLNVNKYNIESFGRVTGYY
                     FCIQEITYIRKLEQNLSKKLREKGQVARYTFDDIKTVSTKMERTKKLGMKIAESDYTI
                     LITGESGTGKELMAQSIHNASPRHSQSFIAINCAAMPENLLESELFGYEEGAFTGALK
                     GGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLRVLQENQVMRVGGENVIDIDVRVIAA
                     TNKNLLEMIKGDRFRADLYYRINVLPINIPPLRERKEDIEVMLKHFMKRKRAISQGVQ
                     DIINAYEWPGNIRELKNTAMYINIMSSEDVILINDLPHNLLNITEDFSKEISILKDRT
                     SIEKVKLVMECLKNANNLNKNIGRNGIVNSINELGYSITEGEVRGILNMLKELDLIIC
                     ESGRKGSELNRRGKNILNSI"
     gene            1485..2231
                     /locus_tag="KR440_003383"
     CDS             1485..2231
                     /locus_tag="KR440_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBH0700386.1"
                     /translation="MKKLVKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            2272..3591
                     /locus_tag="KR440_003384"
     CDS             2272..3591
                     /locus_tag="KR440_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH0700387.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLIKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYADKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
BASE COUNT         1456 a          385 c          732 g         1257 t
ORIGIN      
        1 aattagagaa tgagaatatt aaaaagattt ttagtttgag taaggcacat gatgaagtta
       61 taaaatttaa taataagtac ttaaacgtaa ataagtataa tatagagagt tttggaaggg
      121 ttactgggta ttatttttgt attcaagaaa taacttatat aagaaaattg gagcaaaacc
      181 tatcaaagaa gcttagagaa aagggacaag ttgctagata tacttttgat gatataaaaa
      241 cagtttcaac gaagatggaa agaactaaga aattaggtat gaaaatagct gaatctgatt
      301 atacaattct tataacggga gaaagtggaa caggaaaaga acttatggct cagtctattc
      361 ataatgcatc accaagacat agtcagtcat ttatagcgat aaactgtgca gctatgccag
      421 aaaatttact tgaaagtgaa ctttttggat atgaagaagg tgcttttaca ggagctttaa
      481 aaggaggtaa aaaaggatta tttgaacagg ctaacaatgg aactatattt ttggatgaaa
      541 taggagacat gcctatatac ttacaaagca aattacttag agtattacaa gagaatcaag
      601 taatgagggt tggaggagaa aatgtaatag atatagatgt tagagtaatt gcggctacaa
      661 ataaaaattt attagaaatg ataaaaggag atagatttag agcagattta tactatagga
      721 taaatgtatt gcccataaat ataccacctt taagagagcg aaaagaagat atagaagtta
      781 tgttaaagca ttttatgaaa aggaagagag caattagtca aggtgttcaa gacattatta
      841 atgcatatga atggccaggt aatataagag aattaaaaaa tactgctatg tatattaaca
      901 ttatgagttc tgaggatgtt attttaataa atgatttacc tcataactta ttaaatataa
      961 ctgaagattt tagtaaagaa ataagcatat taaaggatag aacatctata gagaaagtaa
     1021 agttagtaat ggagtgttta aaaaatgcca ataatcttaa taaaaatata ggtagaaatg
     1081 gtattgttaa ttcaataaat gaattaggct acagtataac agaaggtgag gtaaggggaa
     1141 tacttaatat gttaaaagag ctggatttga taatatgtga atctggtaga aagggaagtg
     1201 aattaaatag aaggggaaaa aatatattaa acagtattta ataggttgat aaaaaggaaa
     1261 atctaaatgc ctattttacc tattagttgt acttaaaaca atttaaaaaa gtcaaactaa
     1321 acaggattaa aataaaatat aagtaaataa agttatttat attacttgta ttcaaaaaaa
     1381 gtatttctaa aatgaattat aatgttcaaa taaataaaat cttggtatgc tacttgctct
     1441 ttaagagaag tagcattttt tattgttcac aaataagtta aggggtgaag aaattggtta
     1501 aaaagaaata tccaataata ggaatatcag gtaatttact aattgatgaa ggaggaatgt
     1561 ttcctggcta tgaaagggct tatgtaaata atgattatat acaatcagta gttatgtgta
     1621 aagctatacc atatattgta ccgattgtat atgatgatga gattataaag gagcaggtat
     1681 ctaatataga tgcgcttata ctatctgggg gacaagatgt aaatccactc atatggaaag
     1741 aagaacctca taataagttg ggggcaatat caccaaagag agatagtttt gatatgaaat
     1801 tattaaaaca tgctttagat atgaaaaaac cagttttagg tatatgtaga ggagaacaaa
     1861 ttataaatgt aactgaagga ggtagtttgt atcaagattt atctttaatt gaaggtgctt
     1921 atattaaaca caatcagcaa catctatcta atgtacctac acatacagta cagataaagg
     1981 aggggacaaa attatatgaa atattaggag aaaaagaagt tttagttaat agttttcatc
     2041 acttagttgt caacaaagta gcaccaggat atattgtttc agctacatct aaagacggtt
     2101 taattgaagc tatagaaaag gaaggaagtg agtttgtaat agggatacaa tggcatccag
     2161 aaatgatgac tagagattat gataatatga aaaaaatatt tatggcaata gttaaagaag
     2221 catctaaata aaaaatataa atatattaag ttggctttgg ggggaataac aatggaaaaa
     2281 ggcattaagt taggtttttg gagtattgta ctattaggaa tcaatgcaat aatagggtca
     2341 ggtatattcg ggttacctgg tgatgcttat acagatatag gtccagcaag tatattagta
     2401 ttagtttttt gtatgctttt agctgtttct attgctttat gttttgctga agcaggtagt
     2461 tggtttgata ctgatggagg gccttatctt tatgcaaaag aggcatttgg agattttgtt
     2521 ggatttgaag ttggatttat gaagtggata gttagtatga ttgcttgggc tactatggca
     2581 aatttttttg ctgtaacact atcatctgta tggccacaag cagcagaacc acttattaaa
     2641 aatataataa ttggtatatt agttgtagga cttgggatta taaattttat gggaatgaaa
     2701 caatctaagc acttaaataa tatcatgact ataggaaaat tattaccaat agttttattt
     2761 atagctgtag gattattctt tataaagggt tctaatttta ccccatttgt aataatacaa
     2821 aagggtcaaa gtgcgtcatc agcttttgta gcagtatcta taactttatt ttatgctttt
     2881 acaggatttg aatctttagc tgttgcagca aaagatatgg agaaccctaa gaaaaatgta
     2941 cctaaagcat tggtaatggt aatgtttgta gtatcagtaa tatatatgct tatactggga
     3001 ataagtatag gggtgttggg taatggcttg gcagggtcag ctactcctgt tgcagatgct
     3061 gccattaaga tgctaggtcc tataggtggg tatataatta caataggaac tatagtatct
     3121 gttggaggta taaatattgc atcatccata tttacaccaa ggtctgctgc tgctttggtt
     3181 gaacaaggat taatgcctaa gtctataaga aaaactaata aaaatggagc tccttatata
     3241 gcaattatag tatctgtcat aggaacttta ctaatagcat ggtcgggttc attcacaaca
     3301 ttatctcaaa taagtgttgt atctagattt attcaatata ttcctacatg tttagctgta
     3361 ctaatattga gaaagaagta tgctgataaa gatgtaaatt ttagaatacc tggaggagca
     3421 attataccaa tatttgcagt tattataagt atattattac tgataaaagc aggtattgat
     3481 gaacctatga agatagtttg gggattaggt gggatgataa ttgtagttcc tatatatttc
     3541 tatatgacta aagtatattc aaagaaatat aatgatgtag aagtcaaata attattgtaa
     3601 tctatagata ttctataaat gttattttga atttagtaaa attggtataa caccctggtg
     3661 ttataccaat ttttatataa ataactttat ttataatata tcattatact ttcgtaaggt
     3721 tttatttcca ttttattttc aattatatct ctagtgtaat tggtttcaag tattgtgtag
     3781 tttaatttat tcaaataagt ttcaggtaat ttatatacta cattatcatg
//
LOCUS       DAEPOZ010000285         3635 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.286, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000285 DAEPOZ010000000
VERSION     DAEPOZ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.286"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            73..687
                     /locus_tag="KR440_003385"
     CDS             73..687
                     /locus_tag="KR440_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBH0700388.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            705..1067
                     /locus_tag="KR440_003386"
     CDS             705..1067
                     /locus_tag="KR440_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0700389.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYKGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            1173..1670
                     /locus_tag="KR440_003387"
     CDS             1173..1670
                     /locus_tag="KR440_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HBH0700390.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
     gene            2094..2492
                     /locus_tag="KR440_003388"
     CDS             2094..2492
                     /locus_tag="KR440_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH0700391.1"
                     /translation="MIKKIEHVVITNNNIENYINFYKKFGFSSKEMSEKYKLKARNVK
                     INLNIKGYKFLPYVKNLKASRDNLCFKINTNIDEPKKYLEQNEIQIKRGIVECTGVFG
                     NMKSIYLKKTNGNLIELSNHRITESKPWKI"
     gene            2480..3418
                     /locus_tag="KR440_003389"
     CDS             2480..3418
                     /locus_tag="KR440_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBH0700392.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIRH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHIKTIILGVCGKLILVPSIFLPISIFLGFRNVELTSLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
BASE COUNT         1391 a          412 c          534 g         1298 t
ORIGIN      
        1 ctaaattaaa caaacttttt tacaaataaa tacataaaat ttaaactaat ctatttattg
       61 gaggagataa aattggaaga agtaattgca aaaattatta ctatagaaaa tggattcaaa
      121 gaaattgaaa acattgctaa aaaaattgta aaaaataatg actcaaaaaa gtgttattat
      181 ctatcaatct acttatatag gtcagaatat tatcaagtaa gagaattggc tgtgtttata
      241 cttggtttta tatcagtaac tatttctgaa gctttgttat ttttaaaaga caatgtgagt
      301 aaagatgaaa actggagagt acaagaaata ctagctaaat catttgatta ttattgcagt
      361 gaagtaggat atgaaaaggc acttcctgtt ataaaagaat ggcttaattc tgatagtcca
      421 aacataaaaa gggctgtgac agaaggtctt agaatttgga ctagtagaga ctattttaaa
      481 tctaatcctg acgttgctat atcactactt tctagtttaa aagaagatga tagcgaatac
      541 ttgagaaagt ctgtaggtaa tgctcttaga gatataagta aaaaatatcc agatttagta
      601 aaaaaagaat tagattcatg ggatatatca aataaaaaag ttgagtttgt atataagctt
      661 gcttcaaaat gtatcttggc aaagtaaaat ggaggtgaac ttatttgaaa cttcgtgatg
      721 agtatacttg cccattagaa ctaacacatg atatgataaa aggcaaatgg aaatctatta
      781 tattatggag attacgcata ggaccaacct ccttatcaaa acttgaaaag gaaatcaatg
      841 gtattaatca aaagatgtta cttgaacaac ttaaagaatt aattgaattt ggtcttgtag
      901 ataaaaagac atataaaggt tacccactta aagttgaata ctcacttaca cctattcgag
      961 ggaaacaaat attggatgct ttggctataa tgcaagatat aggaattagc tttatgttgg
     1021 ataatggtat ggaagatatt cttaaggaaa aaggtgtttt aaaataaata gatacccaca
     1081 aaaaagtaag taatttacca ttattttcaa ctggtatata attaaattat agatatattc
     1141 tattaattta ttataatagg aggtaaatta ttatgaaagt aactagtaca ggtatagtaa
     1201 atggtgttat agaagataaa tacggtaaaa gaggaactca gttcaatgaa ggtggtatgc
     1261 ctacatattc actacctcta aagtttgaag atgctccaaa gaatacagta tcctttgctg
     1321 tatttttaga agataaagac tctgttcctg tatgtgggtt tgcatggata cattggttag
     1381 ctgtaaatat aacaaaagat gagttaaatg aaaatgaaag tgtaacagct actgattttg
     1441 tacaagggac tactagttgg catggcaaac taggaggtct tgacagatta gaggccagtg
     1501 tatatggtgg aatggctcct ccagacagac ctcatgtcta tgaaatacat gtatttgcac
     1561 ttgatactat ccttgacctt gaaaaaggat tctatatgaa tgaatttttc aaagctattg
     1621 aagggcatgt tttagataca tttacactaa aaggaagcta ttctaattaa ttaggtttac
     1681 tatagctaat cattaattaa atgctacatt taattgatga ttagcttaat tgttattaga
     1741 atattttctg cactttatct taatatatat tttagttgac tggtatttta atttctatta
     1801 gattattatc aatttatata aattgatttt agcttaaaac ttacttatct atttcagttt
     1861 ctattagaat tattattaga ctcattaaaa taaaagcatt aatataacta ataatatatg
     1921 ttaaattcat taactctaca taaagtttta taatctaaat gaaatacaat aatctgcata
     1981 aaattcaata gcttatataa aatttagtaa tttatataaa tattatcaat ctatataaaa
     2041 tttaataact tatataaatt taactaattt atataaaatc ttaaggagtt gccatgataa
     2101 aaaaaataga gcatgtggtc attactaata ataatataga aaattatatc aatttttata
     2161 aaaaatttgg tttttcatct aaagaaatga gcgaaaagta taaacttaag gctagaaatg
     2221 ttaagataaa tcttaacata aaaggttata aatttttacc ttatgttaaa aatttaaaag
     2281 caagtagaga taacttatgt tttaaaataa acacaaatat tgatgagcca aaaaaatact
     2341 tagaacaaaa tgagattcaa attaagcgag gaattgtaga atgtacaggt gtctttggta
     2401 atatgaaatc tatttatttg aaaaaaacta atggaaattt aattgaatta tcaaatcata
     2461 gaatcacgga gagtaaacct tggaaaattt aatcctatca ataaatgttg ttcttccttt
     2521 gtttctcaca atgtcacttg gatatgttct aaaaaaatta ggtctatttg atactcatac
     2581 tttaaataaa atgaataatg tctgcttcaa gtctttcttg ccattattat tattttttaa
     2641 tatatacaat acagatttag atggttctct taattttaag cttatggctg tagctgttat
     2701 aattattttt tcaatcttct taatcttagt ctttattatt cctaaaattg aaaaagataa
     2761 taagacaagg ggtgcattaa tacaaggtgt atttcgtagc aatttcgtta tatttggtat
     2821 tcctgtgaca atttcactat ttggcgataa gagtattgga gtcacatctt tattaattgc
     2881 agttattgta cctatgttta atgtattatc tgttgtaatt cttgaaattt ttagaggtgg
     2941 caaagtaaat ataagacata tgattaaaag cgtcttaaca aatccactca taatcgcctc
     3001 ttttatagga ttgacaatgc taattttaaa tattaatata cctacatttt tacacaaaac
     3061 tattggtgat atttctaaaa tagctactcc tttatcactt ataattctag gcggatcttt
     3121 tagtttttct gctataaagg ggcatataaa aaccattata ttgggcgtat gtggaaaact
     3181 tatacttgta ccttctatat ttttaccaat tagcattttt cttggattta gaaatgtaga
     3241 attaacttca cttatgataa tgttatctgc tccaactgca gtttcttcat ttacaatggc
     3301 acaacagatg gattctgaca gtgaattagc tggacaaatt gtagtattta cttctggatt
     3361 ttctataatt acagtatttt taataatatt tattttaaag cagaataatc ttatttaatg
     3421 atatttgata acacaaattg gtattatgag aaacatatat aaaattgatt taattgcgta
     3481 aaaacatatt attatactat ttttactata taatataact ttgtgtatat tatctcatta
     3541 aacatggtga aagtagtgtt gctatatatt ttttagtttg aaaaaaatat ataaatataa
     3601 acaatatata aatacaaata ataaatgcca acaaa
//
LOCUS       DAEPOZ010000286         3625 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.287, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000286 DAEPOZ010000000
VERSION     DAEPOZ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.287"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..3495
                     /gene="tcdA"
                     /locus_tag="KR440_003390"
                     /gene_synonym="toxA"
     CDS             <1..3495
                     /gene="tcdA"
                     /locus_tag="KR440_003390"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HBH0700393.1"
                     /translation="KNQVKVNGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLNNIS
                     FWKLFGFENINFVIDKYFTLVGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSG
                     NGRNVVVEPIYNPDTGEDISTSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYY
                     SNEYYPEIIVLNPNTFHKKVNINLDSSSFEYKWSTEGSDFILVRYLEESNKKILQKIR
                     IKGILSNTQSFNKMSIDFKDIKKLSLGYIMSNFKSFNSENELDRDHLGFKIIDNKTYY
                     YDEDSKLVKGLININNSLFYFDPIESNLVTGWQTINGKKYYFDINTGAASTSYKIING
                     KHFYFNNNGVMQLGVFKGPDGFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDN
                     DSKAVTGWRIINNEKYYFNPNNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRY
                     YFDTDTAIAFNGYKTIDGKHFYFDSDCVVKIGVFSGSNGFEYFAPANTYNNNIEGQAI
                     VYQSKFLTLNGKKYYFDNNSKAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFN
                     TNTAEAATGWQTIDGKKYYFNTNTSIASTGYTIINGKYFYFNTDGIMQIGVFKVPNGF
                     EYFAPANTHNNNIEGQAILYQNKFLTLNGKKYYFGSDSKAITGWQTIDGKKYYFNPNN
                     AIAATHLCTINNDKYYFSYDGILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEY
                     FAPANTHNNNIEGQAIVYQNKFLTLNGKKYYFDNDSKAVTGWQTIDSKKYYFNLNTAV
                     AVTGWQTIDGEKYYFNLNTAEAATGWQTIDGKRYYFNTNTYIASTGYTIINGKHFYFN
                     TDGIMQIGVFKGPDGFEYFAPANTHNNNIEGQAILYQNKFLTLNGKKYYFGSDSKAVT
                     GLRTIDGKKYYFNTNTAVAVTGWQTINGKKYYFNTNTYIASTGYTIISGKHFYFNTDG
                     IMQIGVFKGPDGFEYFAPANTDANNIEGQAIRYQNRFLYLHDNIYYFGNDSKAATGWA
                     TIDGNRYYFEPNTAMGANGYKTIDNKNFYFRNGLPQIGVFKGPNGFEYFAPANTDANN
                     IDGQAIRYQNRFLHLLGKIYYFGNNSKAVTGWQTINSKVYYFMPDTAMAAAGGLFEID
                     GVIYFFGVDGVKAPGIYG"
BASE COUNT         1349 a          446 c          572 g         1258 t
ORIGIN      
        1 aaaaatcaag taaaagtaaa tggattatat ttaaatgaat ccgtatactc atcttacctt
       61 gattttgtga aaaattcaga tggacaccat aatacttcta attttatgaa tttatttttg
      121 aacaatataa gtttctggaa attgtttggg tttgaaaata taaattttgt aatcgataaa
      181 tactttaccc ttgttggtaa aactaatctt ggatatgtag aatttatttg tgacaataat
      241 aaaaatatag atatatattt tggtgaatgg aaaacatcgt catctaaaag cactatattt
      301 agcggaaatg gtagaaatgt tgtagtagag cctatatata atcctgatac gggtgaagat
      361 atatctactt cactagattt ttcctatgaa cctctctatg gaatagatag atatatcaat
      421 aaagtattga tagcacctga tttatataca agtttaataa atattaatac caattattat
      481 tcaaatgagt actaccctga gattatagtt cttaacccaa atacattcca caaaaaagta
      541 aatataaatt tagatagttc ttcttttgag tataaatggt ctacagaagg aagtgacttt
      601 attttagtta gatacttaga agaaagtaat aaaaaaatat tacaaaaaat aagaatcaaa
      661 ggtatcttat ctaatactca atcatttaat aaaatgagta tagattttaa agatattaaa
      721 aaactatcat taggatatat aatgagtaat tttaaatcat ttaattctga aaatgaatta
      781 gatagagatc atttaggatt taaaataata gataataaaa cttattacta tgatgaagat
      841 agtaaattag ttaaaggatt aatcaatata aataattcat tattctattt tgatcctata
      901 gaatctaact tagtaactgg atggcaaact atcaatggta aaaaatatta ttttgatata
      961 aatactggag cagcttcaac tagttataaa attattaatg gtaaacactt ttattttaat
     1021 aataatggtg tgatgcagtt aggagtattt aaaggacctg atggatttga gtattttgca
     1081 cctgccaata ctcagaataa taacatagaa ggtcaggcta tagtttatca aagtaaattc
     1141 ttaactttga atggcaaaaa atattatttt gataatgact caaaagcagt cactggatgg
     1201 aggattatta acaatgagaa atattacttt aatcctaata atgctattgc tgcagtcgga
     1261 ttgcaagtaa ttgacaataa taagtattat ttcaatcctg acactgctat catctcaaaa
     1321 ggttggcaga ctgttaatgg tagtagatac tactttgata ctgataccgc tattgccttt
     1381 aatggttata aaactattga tggtaaacac ttttattttg atagtgattg tgtagtgaaa
     1441 ataggtgtgt ttagtggctc taatggattt gaatatttcg cacctgctaa tacttataat
     1501 aataacatag aaggtcaggc tatagtttat caaagtaaat tcttaacttt gaatggtaaa
     1561 aaatattact ttgataataa ctcaaaagca gttaccggat ggcaaactat tgatagtaaa
     1621 aaatattact ttaatactaa cactgctgaa gcagctactg gatggcaaac tattgatggt
     1681 aaaaagtatt actttaatac taacactgct gaagcagcta ctggatggca aactattgat
     1741 ggtaaaaaat attactttaa tactaacact tctatagctt caactggtta tacaattatt
     1801 aatggtaaat atttttattt taatactgat ggtattatgc agataggagt gtttaaagta
     1861 cctaatggat ttgaatactt tgcacctgct aatactcata ataataacat agaaggtcaa
     1921 gctatacttt accaaaataa attcttaact ttgaatggta aaaaatatta ctttggtagt
     1981 gactcaaaag caattactgg atggcaaacc attgatggta aaaaatatta ctttaatcct
     2041 aataatgcta ttgctgcgac tcatctatgc actataaata acgacaagta ttactttagt
     2101 tatgatggaa ttcttcaaaa tggatatatt actattgaaa gaaataattt ctattttgat
     2161 gctaataatg aatctaaaat ggtaacagga gtatttaaag gacctaatgg atttgagtat
     2221 tttgcacctg ctaatactca taataataac atagaaggtc aggctatagt ttaccagaat
     2281 aaattcttaa ctttgaatgg caaaaaatat tattttgata atgactcaaa agcagttact
     2341 ggatggcaaa ctattgatag taaaaaatat tactttaatc ttaacactgc tgttgcagtt
     2401 actggatggc aaactattga tggtgaaaaa tattacttta atcttaacac tgctgaagca
     2461 gctactggat ggcaaactat tgatggtaaa agatactact ttaatactaa cacttatata
     2521 gcttcaactg gttatacgat tattaatggt aaacattttt attttaatac tgatggtatt
     2581 atgcagatag gagtgtttaa aggacctgat ggatttgaat actttgcacc tgctaatact
     2641 cataataata acatagaagg tcaagctata ctttaccaaa ataaattctt aactttgaat
     2701 ggtaaaaaat attactttgg tagtgactca aaagcagtta ccggattgcg aactattgat
     2761 ggtaaaaaat attactttaa tactaacact gctgttgcag ttactggatg gcaaactatt
     2821 aatggtaaaa aatactactt taatactaac acttatatag cttcaactgg ttatacaatt
     2881 attagtggta aacattttta ttttaatact gatggtatta tgcagatagg agtgtttaaa
     2941 ggacctgatg gatttgaata ctttgcacct gctaatacgg atgctaacaa catagaaggt
     3001 caagctatac gttatcaaaa tagattccta tatttacatg acaatatata ttactttggc
     3061 aatgattcaa aagcggctac tggttgggca actattgatg gtaatagata ttacttcgag
     3121 cctaatacag ctatgggtgc gaatggttat aaaactattg ataataaaaa tttttacttt
     3181 agaaatggtt tacctcagat aggagtgttt aaaggaccta atggatttga atactttgca
     3241 cctgctaata cggatgctaa caatatagat ggtcaagcta tacgttatca aaatagattc
     3301 ctacatttac ttggaaaaat atattacttt ggtaataact caaaagcagt tactggatgg
     3361 caaactatta atagtaaagt atattacttt atgcctgata ctgctatggc tgcagctggt
     3421 ggacttttcg agattgatgg tgttatatat ttctttggtg ttgatggagt aaaagcccct
     3481 gggatatatg gctaaaatat atgtttgaca aaaaattatt cctgtgatac tcagaaatta
     3541 tttttatata ataaatattg agatttaatt aaaagtcatg tgttattgta atacatgact
     3601 tttagttaaa atttttctat cattt
//
LOCUS       DAEPOZ010000287         3605 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.288, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000287 DAEPOZ010000000
VERSION     DAEPOZ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.288"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(219..881)
                     /locus_tag="KR440_003391"
                     /pseudo
     CDS             complement(219..881)
                     /locus_tag="KR440_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            complement(1068..1577)
                     /locus_tag="KR440_003392"
     CDS             complement(1068..1577)
                     /locus_tag="KR440_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBH0700394.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLE"
     gene            complement(1652..2065)
                     /locus_tag="KR440_003393"
     CDS             complement(1652..2065)
                     /locus_tag="KR440_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBH0700395.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNSDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            2502..3161
                     /locus_tag="KR440_003394"
     CDS             2502..3161
                     /locus_tag="KR440_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HBH0700396.1"
                     /translation="MDEKILELLQEIQGNISNMQGSISGIEGTVNGMQGNINYMQGNI
                     NYMQGTISDMQGTISDMQGSINGIEGTISDMQGSINGIEGTISDMQSNINDIEGTISD
                     MQGSISSMDGTINSMQDSITDMQGEIKEVNSRLDNMEPDIKDLKTGQVETSSRLDTIQ
                     SDVTEIKSDVKINRNTLDTVIKHTSSLVEDTSDIKNDLNNIEIVTSKNWNDIAKLKSV
                     K"
BASE COUNT         1277 a          647 c          481 g         1200 t
ORIGIN      
        1 accttatacc atctaaccaa ttttttctac tttcgtaact ttcacactta ggtttatttc
       61 atccttatgt tttagtttgg ttagctttag gggtttccag caattcaagt agtattggat
      121 aggttctatc ctatctctac atataggttt ccctatatgc tgactatttt agctactaac
      181 tcatgactaa agtcacaagt gtgcgtagct attttttaat caaactggaa tacagactcc
      241 attcctagga aattggctga aatatagtct gctgtcaaag atgctgtcat cacagaaatt
      301 aaaacagata ctgaaaagtt tttatggact tcttcaagtg aaaacataac acctgccagt
      361 ggtgcatgaa aggctgctga taatccagca cttgccccac aggttaacag gaatttttcc
      421 tctgtcttcc ctcggtcaag agctctagaa atacctttac ctgccatagc accaagctga
      481 atggaaggac cttcccttcc caaagcaaga ccacccataa gtgataagaa tcctccaata
      541 aattttgtgg gcaacactcg ccaccatact tgttttattt tccctgtcat ttccccctca
      601 agctgaggga taccacttcc agaaatcatt ggctcccagt ccaccaactt tgatacaatc
      661 cacgcaagta ctgcaagtac tgcaaaccaa gctattattt ttattgtaga accttctaca
      721 aattttaaaa tttggctaag ccaagtacca gcctgcccta aaagtatcct gtataaaagc
      781 acaatcaaac cagccacagc cccaaccata agtccttcac caatcaaaat tacctgaaag
      841 tgctctgccc gtttgataat atgagatgta tcctttttca tactgttcct ccttcatcca
      901 ataattctac acaatctgaa cctccaatga ctaaagtcat gagtgtgtac agatattttt
      961 taatcaacaa aaatagattt ttatttacta aatataagaa aataattgtt caatttctca
     1021 ataaatgtaa atttattctt ttaggtattt attctccaca gtcgtgctta ttccagcaaa
     1081 atatttatta gttcttgctt tctctgtgta tagattttac gattgtcctc aaactgtaat
     1141 gctaattttt gtttatgact atcatagagt tttgctttgt ctgtaaaagt atttagataa
     1201 tctcgaaaat tgatataatt attccatgcg acactattcc attcaacaac atgaatatgg
     1261 tgagtacgag tatttttctc aaaatctccc atagcaaaaa gtaattgttt tggtacatcc
     1321 tgacctcgaa aaacgattcc acattgctct aacctttcga ggtataattt agcttcctct
     1381 aaagaagcaa cacctacaac aatatcaata ataggctttg cgctaatact gggaatagaa
     1441 gtgcttccaa tgtgttgaat atcaatggca gtcttatccc atatactttt caataatata
     1501 atgacatctt ttgcactttt atcccatatc atctgatgtt ccaataattt aaccatacca
     1561 cattttaaac ctatcactat ataccacctc aatataatcc acctttatat atatattaca
     1621 attttttcta tcttgataaa cagtgatcta gctatttcac tttttcttga cactcgctac
     1681 attccctatc atgaatagat ataattcctc cacatttaga acaagtatat ctctccttct
     1741 gcttttgcat aaacttctct aaaccgtact gctgaacaaa tttactattt tccataaggc
     1801 ttgcttgata tctcttgtta taactttttt caagattttt tatgagttta caaggaaaat
     1861 tgaaacattc aaaacaataa aacaaccctt tttgacagac acaatccttt attttacact
     1921 tacggcagtg ttctggcttt cctgtatcgc tgttcaaaca accagcacat ggctttttgt
     1981 gataacaatg cttatagcaa accatacaat tcattccaca aggagcaaac ataatcgtat
     2041 caatactctc ttttggcatt ttcataacat tttactcctt tcaaaaatcc aatttgtcga
     2101 attgttatat attcatattg taacataaaa aatagctaaa aaattcatgc actttcattg
     2161 ccaataaatt ttcaaggaaa tatgaaagtt taaaactaag aataaaaaga aataaaaagg
     2221 aagctactaa tcaaaatatt caaacacaag taatcaatta tgatattacc agcagtttta
     2281 aatacaaata taaccacata tgcctcaaaa acacttatta tagtatctta gacacttaga
     2341 caatacacaa gattcattaa taaaaaagtt ctcaatatag atatatttaa ccatgttttg
     2401 aatagcagat aattatatga ttaaaaatta aatatgacat agcgtttgtg gaatataaca
     2461 cctaaattat gttataataa taaaacagga gtgtgatagt gatggatgaa aaaatattag
     2521 aattattaca ggaaatacaa ggtaatatta gtaatatgca aggtagtatc agtggtatcg
     2581 aaggtactgt taatggcatg cagggtaata ttaactatat gcagggtaat attaactata
     2641 tgcaaggtac tattagcgat atgcaaggta ctattagcga tatgcaaggc agtatcaatg
     2701 gtatcgaagg cactattagc gatatgcaag gcagtatcaa tggtatcgaa ggcactatta
     2761 gcgatatgca aagcaatatc aatgatatcg aaggtactat tagcgatatg caaggcagta
     2821 tcagtagtat ggatggcact attaatagta tgcaagacag tattactgat atgcaaggtg
     2881 aaattaaaga agtcaatagt agattagaca atatggaacc tgatattaaa gacttaaaaa
     2941 ctggacaagt tgaaactagt agtagacttg atacaattca gagtgatgtt acagaaatta
     3001 agtcagatgt aaaaattaac agaaatacat tagatacagt gattaaacat acctcttcat
     3061 tagtagaaga tacatcagat attaaaaatg atttaaacaa tatagagata gtcacatcta
     3121 agaattggaa tgatattgct aaattaaaat cagttaaata atataccata gtaaaaaata
     3181 cctgaaaatt tcacatactt ctttaaagtt ataccaaatt ttatacagaa atactgatta
     3241 tccgacaaca cactttactt ataaataatt tattaaaaaa tactcatttt atcctaaaat
     3301 taacacgact aaatgagtct tgatataata ataaaaaata agggagattg aacactaaaa
     3361 agttgtgtat ctcccttata ttctataata tagttatata atttttcagc ctaaaataaa
     3421 agtccatctt tgattaaaac atatcccctt atcacttata aaaggataaa atcttgtttt
     3481 catttttcca tttatatctt cttgcttaag agtaataatt ttttcttttg atactttaag
     3541 tgtttggtta aatttaacca atcgactaag ttttgttcaa catctatttt tcatattttc
     3601 caata
//
LOCUS       DAEPOZ010000288         3593 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.289, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000288 DAEPOZ010000000
VERSION     DAEPOZ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.289"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..570
                     /locus_tag="KR440_003395"
     CDS             <1..570
                     /locus_tag="KR440_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700397.1"
                     /translation="KVEKLDLPSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKD
                     RSICKFDNTVFDEMSKLSNLEEMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEA
                     VENSIYGYIEIKKDFQLMLTSDGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDF
                     EDNDYKAVKVPRFKIKDDSSCLYLDIYND"
     gene            614..1849
                     /locus_tag="KR440_003396"
     CDS             614..1849
                     /locus_tag="KR440_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBH0700398.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREIVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKSISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYISLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNEKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            1950..2891
                     /locus_tag="KR440_003397"
     CDS             1950..2891
                     /locus_tag="KR440_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HBH0700399.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(3120..3473)
                     /locus_tag="KR440_003398"
     CDS             complement(3120..3473)
                     /locus_tag="KR440_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBH0700400.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMIW
                     LEKLKEDNTYEIYSI"
BASE COUNT         1366 a          331 c          636 g         1260 t
ORIGIN      
        1 aaggttgaaa aattagattt accatctagt tctatagttg ttgtaaaatt tcatgaaaat
       61 aaagtagagt attttttatt aggagattgt gctttgttta ttaaaaatgg agattccaaa
      121 gttataaaag ataggagtat ttgtaaattt gataatactg tatttgatga aatgagtaaa
      181 ctttctaact tggaagagat ggcatttgat gagattagaa ctagtgttat ggatacaata
      241 gtaagcaata gattgaagaa aaatacgaaa gagggctatt ggatattgga ttttgatgag
      301 gaagctgtgg aaaattctat atatggatac attgaaataa aaaaagattt tcaattgatg
      361 ctaacgagtg atggttttac aagtgcatgt gatagatata atctaataaa agaagaagaa
      421 ttgatacgta tagcagaaaa atttggtata ggatatatac ataataaagt aagggatttt
      481 gaagataatg actataaagc ggttaaagtg ccaagattta aaataaaaga tgattcatct
      541 tgtttatatt tagatatata taatgattag actgtataaa actatttaat tttagaaagg
      601 aggcaagtga aaagtgaaaa ttcttcatat aataacacaa aaaccaaata gtacaggtag
      661 tggtatatat ttgtctggta tgataaaagg gttcgaaaaa atcggtcata agcaagctgt
      721 aattgcaggt atagatgtaa atgatgatgt aaattgtttt ccaagtgaag taagttttta
      781 tccagtaaag tataattgtg gggagttaaa ttttcctgtt gtaggtatga gtgattctat
      841 gccatatgaa agtacaagat ataaagattt aaatatagac atgataaata gattgaaata
      901 tcaatttaaa gtaaatatag ataaggctat gaatgatttt aaaccagaac ttataatatg
      961 tcatcatttg tacctcttaa ctgcatttgt tagggaaata gtaaaagata taaaggttat
     1021 gtctatttgt catggaactt gtttaagaca gttaaatacc attgatttag agaaagaata
     1081 cataatagca aatataagga aattagattt aatatttgct cttcatgaaa atcagaaata
     1141 tgatattata aagacttttg gagtaagtga aagtaaagta gttgtaattg gtagtggtta
     1201 taatgatgat atattttata ataaaaatta taaaataaag gatgataaaa taaaaatagt
     1261 atttgcaggt aagatatgta aatcaaaggg attgattcca tttataaaat ctatatctaa
     1321 attaaaatat agtaaagatt taatagaagt caattttgca ggtactggaa gcgatatcga
     1381 atcatataat gagatagtta aactggcaag taagtctcca tttaaaatga atttcctagg
     1441 aaagttagaa cagagagatt tagctgagct attcaacagg tcacaaatat ttgtgttacc
     1501 atcattttat gagggattac cagttgtagt tttagaagct ttatcttgtg gcacagatgt
     1561 aatcactact gatattcttg gtgttaaaga gtggattggt agtgagatta ataattctgg
     1621 aaagatagaa tatatttctc taccttttat ggagaaagaa ggtattccaa aagatgaaga
     1681 gttatatgat tttgaaaata atttgtataa tgcaatagat tctaagatac aatctttatt
     1741 gaataactca aatgagaaaa catctgtaga tatgtcaaaa aaaacgtggg atggcttagc
     1801 atatagaata aatgaagtta ttttaatgga tgaattgtgt ttagtctaaa attatttaaa
     1861 ttataaaaaa gtgtaactat ttttaaactt tttttaaatg attttatatt atactaatac
     1921 taaatatatt tgttttaggg aggtaattta tgaggattag taaaaaggta ttggcgttag
     1981 gtatttcagc attactgtta agtgttagtt ttccaacatc aataaacgca ctagataaga
     2041 ttgaaaatat tcaaggagta gataaatatg aaacagctgg gcttatttca gataagcaag
     2101 attttacaac agcagttctt atcaatgcag attctacaat ggtagatggg attgctgcaa
     2161 gtggtcttgc aggagtaaat aatgcagcta ttttattaac taataaggat gatataccag
     2221 aagcaacact tcaaagactg aatagagtta ctaagatata tgtaataggt ggagaaaatt
     2281 ctataagtaa agatgtagaa aaaatgttac ttatgagaag aatgcaagtt ataagaattg
     2341 atggtgtaga tagagtagat acaagttata aaattgcagg tgaaatagaa aaaattaaaa
     2401 acagtgataa gatgttttta gtaaatggat ttaaggatga agctgatgca gtaagtgttg
     2461 catctgttgc ttatagagat ggagctccta taatattgac aaaagatata ccttcagcag
     2521 aagaagacac agacttagac ccatggtttg gagtttctcc tgttccagta tatgctattg
     2581 gaggagagtc tacattaagt gattacatag tgagcagata tagagcaact agaattggcg
     2641 gtgttgatag atatcaaact aataaaaatg ttattgaaaa gttctataat ggagcaaaag
     2701 agttctatat aacaagtgga gacgatttag tttatgcttt agttgcatca ccacttgcaa
     2761 aaaatgctcc tgttgtttta gtttcaaata aaagtgataa gagtatatta agtggagcta
     2821 gtaaagtaac agcaataggt ataagtgata agagtattat agagcaatgt ttggatgctg
     2881 taaagaagta atggagtgtt attagtaaaa tgaatatata cattgaaaat tagtagattg
     2941 ttcattttat aatggtataa aatatatggg aagaggctat caaatggaga taagcctctt
     3001 ttttattgat aaaattatga aaatatactt tattttatat aaaaaaagta tctcatgaac
     3061 taaatgtcta ttttgatata ctatctcctt ttcttatatt atatgtatta tatgattttt
     3121 tatatggaat aaatttcata agtattatct tcctttaatt tttctaacca aatcatctct
     3181 aaaatatcta gttcttctaa atagtctttc tctgttttgt tatcatcatg ttttataagt
     3241 tctaaaactt caaatgtaaa tgctttttca ccatattttt tccattcatt atttaattct
     3301 tttatcatac atgagccagc accaagctga aatttgagag agttaaattt gctttttatg
     3361 tcattacttt ttccaaggta aatcttttta gtaatatcat tttttattat gaatacacct
     3421 gtgtcaaatt tcatttcttt gtataattgt tttaattcac gttttctatc catgatttta
     3481 gatacctctc tttataattg atatgtaata tgtaagttta aaaatatatt acatatcaat
     3541 tataaagaga ttaattttat atgtaaatag tttttataaa tttaattatg aat
//
LOCUS       DAEPOZ010000289         3559 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.290, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000289 DAEPOZ010000000
VERSION     DAEPOZ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.290"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     repeat_region   847..1804
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=847..875
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaag"
     gene            3021..3176
                     /locus_tag="KR440_003399"
     CDS             3021..3176
                     /locus_tag="KR440_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HBH0700401.1"
                     /translation="MALDVKIEVKIIKDVVILNIIHWKKETSLLTVKIARGQGDLSYV
                     KYFTNES"
BASE COUNT         1422 a          354 c          514 g         1269 t
ORIGIN      
        1 ttagatagta aactttaaaa tatagctatt aattatatga atataataaa aaaagtacta
       61 atgattgaca ctagtacttt ataaccactt ttacatgttt taactgtata aaacagtggg
      121 tatagttcag cacaaagagt gacttacgag ttgaatttaa atttatattt aaatccaaaa
      181 aataaactct gtttatgatg aactccactc taacgcaaaa tagattgtag ttcttcttgc
      241 ttttattata ccacaaaaca ttataaataa aactgttgtt tataaaaaaa tgcaaagtaa
      301 aattttttat aaacaatgat aaaaatttta ttatagagct attaacttat gaaatatctg
      361 taaatgagta gatatttcat aagttaatat ttattgtttg tataaaatat ttggtaaaaa
      421 taagtaagtt ttatatgtta taataattgt agcaagaata ataatcgaaa gcagcaagca
      481 tgtcagctgg tgttatatca gtttatattt atgataaaat aaaaatctcc caaacgccaa
      541 taaggatgat ttaaaaaaat aatattttca cttaaaaata atcactcttt ataggagtaa
      601 attattttct tgattttatt atactacaaa ttggtacaga tatttaaaaa taatatattc
      661 ataatattat aaaattataa atagttttgc agtgagcgat atttttgata aaatagggcc
      721 taacagttga aatataaggc attgagggta tgtgataaat gttatcattt gcactactca
      781 tggttcactg caaatttgag agaattgtat agatgtaagt gttggaaata ctcaatttat
      841 tttggggttt tatattaact aagtggtatg taaagtctcc atctccaatt aacataaata
      901 attcatccgc tggttttata ttaactaagt ggtatgtaaa gccgcttggc aaatcataag
      961 aaggattatt ttctccaacg ttttatatta actaagtggt atgtaaagaa aataaaagag
     1021 tttgaattac ccattttttc atggttttat attaactaag tggtatgtaa agtacgacat
     1081 tgaataaaat gggtacagag acaatttata agttttatat taactaagtg gtatgtaaag
     1141 tttataaatc ttttaatgac tgtctctgat ggtacaggtt ttatattaac taagtggtat
     1201 gtaaagaaag gaatagatga tatgattgct aatgctgaac aacaagtttt atattaacta
     1261 agtggtatgt aaaggggtct gactttaata tatatatata tatatataaa ttgttttata
     1321 ttaactaagt ggtatgtaaa gttataaact cccacgacaa aattaactat aacctcgtgt
     1381 tttatattaa ctaagtggta tgtaaagagt tttaaagagg gcagacctaa aaaatatact
     1441 aaaagtttta tattaactaa gtggtatgta aaggcagaag atttagcgac tgttgttgct
     1501 gaatgggatg gttttatatt aactaagtgg tatgtaaaga ttaaattgct cagtctagta
     1561 cttaaagaat tataaggttt tatattaact aagtggtatg taaagttgct atgatttaat
     1621 tatacgacgt tttgtcttta aaggttttat attaactaag tggtatgtaa agtgctgtgc
     1681 cacaatttga tgcttctata gctaaattcg ttttatatta actaagtggt atgtaaagct
     1741 tttaagtgca tcaaaggagt tacaagctat atttagtttt atattaacta agtggtatgt
     1801 aaagacagat ctaatatcaa aataaggtta tgacttttaa aagcatagcc atatcagtaa
     1861 aaatttaatt atcatttaaa aaataaattt ttatttaaag aatacacata aatatttcca
     1921 gacttaactc tcttaaacca ttttcatatc ctttagtaga tacttttgtt attttttaat
     1981 ctttagtaac ttattcttga gtgaatctct tattttttct aagctatttc aagcttctca
     2041 aaaaattcta tatttatatt catcacacat ataatacaat caattcagtt ttaattgcca
     2101 atatttagtt tttctgtatc tgataaacca agaacataat cagtatataa accaaaaatt
     2161 ttagcaaata ttattaaccc atcgtctctt gttggtcttt caccagattc tattcataac
     2221 acttgtattt atatttgttt tttcaagcaa ttctttttga gaactattca tattttccct
     2281 agtatattta attctttgat ttacatctat acataattac ctcgaaaaat ttctgttttg
     2341 gaaatattgt tattgaaata tagcaattaa ggtgaatata tgaaaaagat acactatgat
     2401 tttataaaaa agaagacata cataaaattt atacttacaa agtgttgcaa aagagttagg
     2461 ttttaaaaat gcatcaactt attttaagta caggaagggg taatattcaa ttagagttga
     2521 tatgttacct ataaactatc taagatactt gattatgata tagaaaattt ttttacaaat
     2581 taaatttcta agatagaaat ttaattaaga ggatgatgag gatgaataat ttacaagtaa
     2641 tataaagaaa taataagaga attttaaatg catagcaact agcagatata tatgaaaatg
     2701 atgtaaacaa tatataaact aacttcaata gaaataaaga gaatgtaaat tatgttttat
     2761 tacaattcga gtatttgaaa gaatttaaga actaacctac taatagtcag ttgatttcaa
     2821 aacattctag tcaattatac ctttggatcg aaagatgtgc aaatagacac tgcaaaatat
     2881 tagatattga taaaacttgg gaacaatttg acaatttaga gaaaatatat ttcaagatta
     2941 agcgagagaa actaagctgt atagaagatg tattaataga aaatttaaga tttaaggata
     3001 caaattaata aagcaataat atggcattag atgtaaaaat agaagttaaa ataataaaag
     3061 atgttgttat attaaatata atacattgga aaaaagaaac aagtctatta acagttaaaa
     3121 ttgctagagg tcaaggtgat ttaagttatg taaaatattt tactaatgag agctaagagc
     3181 ttcttaacaa gagttttggt gtgtctcttg aaacaatgtt aacaattaat aaaagacgaa
     3241 tggcagaaga aggcgtatgt aagtttaaaa gagataagtt aaacaaacta gatactatat
     3301 aagaggataa gaaacttata gaagaatatg ttacttttgt aaaggattta gctttaaaat
     3361 atagaatata aagtgatttt aagtaaaaat tgtaacaatt aaataagtat aagacaagtt
     3421 tatgagttca tattttataa caaggggtga tgttatgaaa atactacaac aatttaaacc
     3481 atacaaatat gataaagaaa aaaatataga atatgatgaa aaaattatag ctaatcaaaa
     3541 cacagaagtt tatatagta
//
LOCUS       DAEPOZ010000290         3557 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.291, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000290 DAEPOZ010000000
VERSION     DAEPOZ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.291"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..894
                     /gene="pilO"
                     /locus_tag="KR440_003400"
     CDS             <1..894
                     /gene="pilO"
                     /locus_tag="KR440_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HBH0700402.1"
                     /translation="FKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQYQKYI
                     ATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLFLIGLD
                     KKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNVTQILD
                     YTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIKEGEIL
                     KNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKENTTMIL
                     DTDNPSSWRPGKYNPFKSSAK"
     gene            921..1487
                     /locus_tag="KR440_003401"
     CDS             921..1487
                     /locus_tag="KR440_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBH0700403.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            1524..2051
                     /locus_tag="KR440_003402"
     CDS             1524..2051
                     /locus_tag="KR440_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBH0700404.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            2072..>3557
                     /locus_tag="KR440_003403"
     CDS             2072..>3557
                     /locus_tag="KR440_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700405.1"
                     /translation="MRKWNKFKSERGAALVLVLIVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRNISDE
                     DFNNQDEKNIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKMFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYIWNSKWDTLENLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKDGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDI"
BASE COUNT         1565 a          334 c          602 g         1056 t
ORIGIN      
        1 tttaaaaaag atataaaaga tatatttaaa atggaagtta gtctaaaagg gattcttata
       61 aaggttataa ttttattagc tattttatca tatggttatt ttggacttta ccctcaatat
      121 cagaaatata ttgctacaaa aaattctgta aaacaggcta ctgaaaaatt aaatatatat
      181 aaagagaaaa ctgcacttat gccaaaatat gaaaaacaac ttgaagaagt tgaaagtgaa
      241 ttaaaagaaa aaagcaaaaa tcttttatat gatatgcaag atggtctttt tttaataggt
      301 ttagataaaa aaatgaaggc ttataatatt gacttaacta gttatgacgt tggaaatgtt
      361 aaagaatacg aacattttta tggtatatca acaacaataa gtgttagagg tgattataga
      421 aatgtcaaaa aattaatggc ttatttagaa gaacaaaaaa atgttactca aatactagat
      481 tatacaatga cttcatatat agaaccagaa aaagaagagg aagaaacaac aaacaataag
      541 gatttaatta aaaatgtgaa cataaataaa gatacaaaaa tatactgggt ggaaggcgaa
      601 aataagtggc atttatataa ggattgtcca ttgataaaag aaggagagat tctaaaaaat
      661 gacaaacttt tagataggtc tactaattat ctatgtaata cttgtgtact tagagctgca
      721 acagaaaaag aagcaaaaaa agaagtcgta gctccaaagg cagaaggtgt agtagatgct
      781 acatttaaat ttataatgta cactaaagag aatactacaa tgatacttga tacagataat
      841 ccatcttcat ggagaccagg aaaatataat ccatttaagt ctagtgctaa ataaagctaa
      901 gtaagcttta aagataggtg atgagtttgt acaaaaataa tgaaaaaggt cttactttat
      961 tagaagtaat aatagctgtt tttatactga caatagtttt aagtatttct tataaagtgt
     1021 tcaatggtat aacatcagca gtaaaaaagc aacagattat tacagatgct caagtaaata
     1081 ttaatctaat taataagtat ctaaatagag atttggaaaa ctgtaaagag ctaactaaaa
     1141 ctggttcagg taataattat gaatacaata tagagatgcc agataatgtt gtaaaatatg
     1201 aagtttctat agaaactaaa aaaaatacag aggtatattc tgtaacgaga atccaaaaca
     1261 atacaattga tacagaaaat gaagttagag aagagataat ctataatcaa ccactagttc
     1321 aaaacaataa agaaatgaag gaaacaccat ttaaaataga aaagcaaact ggtaaatcta
     1381 tttatactgt aagtatatac tataatgaat cagtgcaaga aagtcataaa aatagcaatt
     1441 taaataacaa aacttataca tttgatgtta tgtcaagaat agggtaataa gaatttatta
     1501 aaaaataagt aggttgataa aatatgagta aaaagaatag tgaaagagga ttttccttaa
     1561 ttgaagtgtt agtagctatg gctattatgg gaattgtact atttgcattt tttaatatta
     1621 tcaatactaa taacaaagca aacactaaaa atgacacaga tataacttcc ttaaactatg
     1681 tccaaagtga aatagaaaat ctaagagaaa agataaaaag tggagaattt gattttgata
     1741 gtttagataa actagaagat ggaactgtcg tatatgaaaa attaatagat aaatcaaaaa
     1801 aagttgtata tgataaagtt cttagtgaag gtgacgtgag cttatatgat actccatatg
     1861 aaaagattac aacaataaaa gatgaagatg gtaatttaat cgacaaagga aatattacaa
     1921 ataagataaa gactattgta gaagataaaa gtggtcagat atataaaata gctgtaactg
     1981 ggaaaagcat gaatgactat tcaagtaaaa aagaggttaa aattgtaact gaaatattta
     2041 aagataaata attttacttg ggggtgagat tttgaggaag tggaataaat ttaaaagtga
     2101 aagaggggca gctttagttt tagttttgat tgtagtagca ttgctttcta ttgttggatt
     2161 gatattttca aatcagatag ctaatagaat aaaatctaca aagactacta atgaaggtat
     2221 acaagcaaag tatttagcag agacatgtgt ggaaaatagt attgataaag catatgaaaa
     2281 attgtatgat gagcttgaga aaatggataa tgagtttaaa agtgaaaatc aagaaaaatc
     2341 catatctagg tcaaaattaa gaaatatatc agatgaagat tttaataatc aagatgagaa
     2401 aaatattgaa gcagaaagac ttggatatat gaataatata aatttctatt taaataaagc
     2461 ttcttcagac ttggaaaaag ctagtatgga attgaaaaag ctatatgatt tagatatgtt
     2521 ggactataga gacattgaat atgtagatgc aaatattatt tcacatagag attctatctt
     2581 agaaatttgc aagaattata cttctggaga tattagtaaa ataaatgaat atattctgaa
     2641 agaagatata gactcaacta cattgattga ggcaaagtta gtgaataatg atattctttt
     2701 gaaaatgttt ttagaagaaa ataaaattga aaatgaacat ttaaattcag ccttttcaca
     2761 tacttataag gcattagata acatttcttt ggcgatgcaa aatatgattg agtatagaca
     2821 tacgtttcat atagatgagc caaaagtaga agtatctaat gggattccag attctcaaca
     2881 atattatgaa ctaatacaaa atcctataat aaatagtatg gagtatatct ggaatagtaa
     2941 atgggataca ttagaaaacc tattggaaat tttaccaaat caaactcaag gatttaattc
     3001 tttaagagtt catttgagaa ataatgtaag aaaatttgag aagttaagcg ataatatatc
     3061 tagtggtaaa aaaaatacag caaaaaattt tttaaagtat aaagagttat tgtatgaaat
     3121 atcagaccaa tgtaatcaac taaaaagtat gtcttatgag aaaatacctg ttaaatatga
     3181 taatatggct ttgataacaa cttttgacta tatacaaaat gagcttttag cagagataaa
     3241 atgcagatta aaagaattaa aaccacaaga aattgataaa acagaaggta ttactataaa
     3301 aatacctttt tataaggctg actacgacat gacaaaagaa ggatggccta aattaaaaga
     3361 aaatggttca ggggcggagt taagcttaat ggtaactggt gataaagatg gaataaaaga
     3421 agtagaagtt acagatggta aaaaaaatat tattggttta ggtgttgaag aaaattcaaa
     3481 ttcaaagtat aaggttgacg ccattgtaaa ctttaatcta aatatagata caaatgtagt
     3541 tggaaattat gatataa
//
LOCUS       DAEPOZ010000291         3522 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.292, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000291 DAEPOZ010000000
VERSION     DAEPOZ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.292"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(232..1446)
                     /locus_tag="KR440_003404"
     CDS             complement(232..1446)
                     /locus_tag="KR440_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBH0700406.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYEI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            1861..2577
                     /locus_tag="KR440_003405"
     CDS             1861..2577
                     /locus_tag="KR440_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HBH0700407.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            2712..3338
                     /locus_tag="KR440_003406"
     CDS             2712..3338
                     /locus_tag="KR440_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBH0700408.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
BASE COUNT         1341 a          437 c          540 g         1204 t
ORIGIN      
        1 caaaatattt attgtctaac taattataat ctatccaaaa tgaaaatggg tgtgagatat
       61 tattacaaca aatcgtatta gtattaaaaa agattaaaaa attaaaaaac taaacagtta
      121 aataattaaa cgattaagtg actgtcatat aacaatttat aattttattt aacagccact
      181 taggtattta agagcgatag aattgagtgt atatcaaatt ataataattt cctagtaaat
      241 aattgaaaaa taaggtagtc catttttata tttgatatct ggtttacctt gaataagtgg
      301 atatagataa tttagggctt catcagttat ataattacat tcttcattta tccactcttt
      361 aggaaaatat tttgtcttgt tagctatttt agaaatatca gtatgactaa cttcatactt
      421 ataaggatta ttgcatgttc tattaatgat agccataact cctgtatatc cttttttaga
      481 tagttctatc ccatatgcac caacttcagc tgcttgtttt aaatcagtat ctgaagctaa
      541 atgcatagca catctttgta ttacaccaag ttctaaggtt tttactcttg ttgtaattga
      601 tgatgatact ataagattct ttagatagtt gcttactcca cctaattggg aatgaccaaa
      661 tttatcatga gcagaagttt tttgttcgca tataaagttc ccgtatttat cccttatacc
      721 ttctgatgca actatataag ccttattttt ttctttaaat atttttctaa catcttctat
      781 aaacttatct tcatagaaat caatttctgg aaggtatatt aaatcaacaa gtggagtatc
      841 attaagtcta gctagtgaag aagaagctgc taaccatcca gtatctctac ccatggtttc
      901 tactataaat ataccattat ttttgtatat agaaaaatct aagtatgttt ccattattag
      961 tgtagaaata agctttgcag cagaaccaaa tccaggacag ttgtcagttc ctaataaatc
     1021 attatctatt gttttaggaa tccctataaa ttttatttta caagtagtgt ttttgcagta
     1081 ttgagaaaat ttcatcactg tgtccataga atcatttccg cctatataaa ataatgtatc
     1141 tatttcatat tcatctagta tatttataac tctatcataa tcataagaat ttattttata
     1201 atcagccagt ttgtatctac aagaaccaag accagacgaa ggtgtttgct ttaaatttaa
     1261 tacattatct ttgtttaagg agtctgaatc tattatattt ttgtttaata taccttcaat
     1321 cccattgata ccaaaatata ctttatcaaa gtatttagtt tctaaatttt tttcataaat
     1381 accaactgca gaagcattga taacagctgt agggcctcca gattgggcaa taatacagtt
     1441 tctcatttta catctccctt tcattaatat tgttatgaat ggtacttttt atcgagtaaa
     1501 tttatattta tagtctgatt atatattaaa tgaactagct atatcaattg agttagtata
     1561 gacaataaat gccataagaa tgtggtaaaa aactaacttt ttatatacaa ttaatgtcta
     1621 taatttacca caatataaag gtacttttaa ggagtagtca aagtgttgtg aaaaaaaata
     1681 tatcagtgta atattaattt acaaagcaaa ataagttcac agttaacaaa agcagaataa
     1741 gttcataaaa gaaatatata cagcaataaa agtaatgcta aataaaagta atactggatt
     1801 aaaaaatatc aaaaactaat atttaatact aattaaagta aaatatggag gtatacaaat
     1861 atgaaacaca taaaaatagc agcaggatta gcacatgtaa attacggtca tatatcagcc
     1921 gtagttaaag aagcaatgga tgcaggtgta gactacgtac attcagatgc agcagatatg
     1981 catgacttaa aaaatatgca acttatgggt ggtcatcaaa taatagaagc aataagacca
     2041 gtgacagata aaccaataga atgccatata tacacaagag attgtgatag attatttata
     2101 gaaaaaatag cagcagcagg atgtaacttg ttgataatac cagcagaaca ttttttaggc
     2161 gctcctcttg catacataat aaattactgt agagaatttg gtatgaaagt aggattaaca
     2221 attggatgtt acacaccact atgttttgtt gatgaagcta tatatgatat agatagattg
     2281 caaatagtcg ttcatggtgt tgatgaaact gatggaaaag ataactgggg atggagaaag
     2341 agtgctgttg atttagtaaa aagagctaga aaaatgatag acgaaaagaa tcctaagtgt
     2401 gaattagcaa tagatggtgg actaagagca gacaatatgg agccattaat agaatgtaat
     2461 ccagacgttg taatattatc ttcagcaata ttcaaggata aagatggtat aacagcaggt
     2521 gttaagaatt gtagaaaagc tatagatgaa gcagctacaa agtttggttt agaataagat
     2581 aataaactat gataaaagag atgctttctt aatgttttaa ggaagcatta attttatgaa
     2641 aaactacagg aagctagact aatttaattg attttttata ctgaaacaat ataatcatag
     2701 gaggttatta aatgaaaaaa gaaatgataa aagattattt actgataaca atatcagcat
     2761 ttttaatggc attagcagtt aattttttct ttgcagaaca tacattagct ccaggaggga
     2821 taacaggatt atcagtcgta cttagcacat tcttatcaat gcctgttgag aatatatcat
     2881 taggaatatc tatgccactt ttagtaatgg gattattatt tttagggaaa ggttttggaa
     2941 taaaaacatt atatataact cttatgggtc cattattctt aaaagtaata ccacaaactc
     3001 acataactga caatctatta gtagcaggag tgataggtgg gttattggta ggaactgcta
     3061 tagggatagc aataataagg ggctgtagta ctggtggaac tgatttagtt gcaatgctta
     3121 ttaataaggt aattaagttt ctgaagttgc cagttatatt atttatgcta gatggattta
     3181 taatagtagc atctggaata ataagtaaga attttatgat atctatatat tcattgatat
     3241 ccttattact cataataaaa acaataggat ttgtaactac aaagtttggt aagaatactg
     3301 aagaaattaa taatataaat aatgcttgcg ctcaataaaa ataataaaat ttactttaaa
     3361 tataaaagtt taaaaaatat aaaagtttaa aaaatataaa agcttaaata aaaagattaa
     3421 atataaaaaa tgaaggtgtc tcaaaatgaa ttttttagtt catgaggcac tttttctatg
     3481 aaatcaaata tatttttatc atttggacaa tgaaaaatag gt
//
LOCUS       DAEPOZ010000292         3515 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.293, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000292 DAEPOZ010000000
VERSION     DAEPOZ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.293"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            53..706
                     /locus_tag="KR440_003407"
     CDS             53..706
                     /locus_tag="KR440_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBH0700409.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNSFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDEIRGAIFDVSQAIDLTKLMGIIIK"
     gene            847..2118
                     /gene="brnQ"
                     /locus_tag="KR440_003408"
     CDS             847..2118
                     /gene="brnQ"
                     /locus_tag="KR440_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBH0700410.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAIFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            2320..3507
                     /locus_tag="KR440_003409"
     CDS             2320..3507
                     /locus_tag="KR440_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HBH0700411.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMTKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
BASE COUNT         1298 a          386 c          653 g         1178 t
ORIGIN      
        1 tatattaaaa aaaataatat caaaaataat ataatttata gtggagggga aaatgactaa
       61 aattattaaa gtgcaaaaac ctaaatttac aggagaatta gaaattattg aaaatcttga
      121 agatatactt gaagatatat ttaatgatga aaaaatattt aataaaatta tagaaaatga
      181 agttataagt gatttagatt ctgtaagagt tagctttgaa tcttgtatat ttagaaatgt
      241 atcctttgaa aattgcaatt taaaaaagat tgatttactt gatgtggtat ttgaaaactg
      301 caatttatca aattcatttt ttgatggagg aagtatatac agagttgaat ttaaaaatag
      361 taaattgata ggagttagat ttgatgattg tatacttaaa aacgttttgt tcaaggatgt
      421 tttaggtaag tattctaact tttcattctc taaatttaag atagtttcta tcttagattc
      481 aaattttgaa aattctgtat ttcaagaagt aaaaggtgac aatctagtat ttgagtatac
      541 aaacttaaaa aaagcaaact ttaatggaac taaactttct ggcatagatt ttaccactaa
      601 taatattgag ggaattgaga ttggtataga tgagattaga ggagcaattt ttgatgtgtc
      661 acaagcaatt gatttaacga aattgatggg aattatcata aaatgaaaaa aatattcata
      721 aaataaattg ggaaaacgtt aacaatgtgt gaatttttac cagattgaca tttttctgaa
      781 aataaagtaa gattatctta atattcaaaa tattttcaaa gggggtaatt tagtgtgagt
      841 aagcagatga gagatatttt tatagttggt tttgctttat tttctatgtt ctttggtgca
      901 ggaaatctta tgtttcctcc atttttaggg atggaatccg gcaaagactg gttaattcct
      961 cttattggat ttgttgttgc agatgctggt atagcaatgc ttgttattat agcaacagca
     1021 agatgcaagg gaagcatgga tgatgtactt aggagagctg gtaaagggtt agcaagagta
     1081 atgagtgtag ctgctctagc atgtttagca cttcttgtaa taccaagaac ttgtgcaaca
     1141 acatatgaaa tgggtattat gcctatattt ggagaccatg gaccagtagc taaagctata
     1201 ttttcaataa tcttctttgg gttaacttta gcatttacta ttaggtcttc taaggttata
     1261 gacataatag gtaagtattt aactccagcg ttattattag tattatttgt tttaatagta
     1321 aagggtataa tttctccagc aggacctatg agtcctgagc atatgataga taaaaatcta
     1381 tttggtgaag gtatatcaca aggttatcaa acaatggacg ctctaggtgc agcatctatg
     1441 gcaactataa tgttgatgtc tataatagct aaaggatata catcagataa agaccaaata
     1501 ggaatgacta ttaaagctgg ttttgtagcc tgtgtatttc tagcagtagt atatggtggt
     1561 cttacttatt taggtgcaac agtttcaact ttatatgata ctagtatacc tcaagcatct
     1621 ttattagttg aaataactag tatattgtta tatggtcaag taggaaaggt aattttaggt
     1681 gtaattgtgg cacttgcctg tctgactact tctacaggtt taacagcatc aatatcaact
     1741 tattttgaag gaataagtaa ggcgagttat aaacaactgg ttataggtat atgtgtagct
     1801 agtatgctta tatcaaattt aggtgttgat tctataatag ctatatcagt tccaatccta
     1861 caaactatat atcctgtttt attagcaata gttgttatgg agttggctgg taaacatata
     1921 aagaatgata atgcttttaa aggtgcagca tatgttacat tagtgattag tctattatca
     1981 gctataaatg gaatgacagg agctgttcca tttatacaaa gcttgccact cgcaggtttg
     2041 ggctttaact gggtaatacc agctatattg ggtggaatag ttggtaactt tataaaaagc
     2101 aacaaacaag tagcataata atttgtaatt aatttaattt tataatatat taatttttag
     2161 tactatgggt aggtttaatc taaacctaat ctacccatag tactttttag attggattgg
     2221 aatagcatgt atataatttt ttataatata atttttcttt gaagtatgat aaaattagta
     2281 atagtgataa gaaagtacaa atttaggagg ttttaattaa tgcttagaaa aataacagca
     2341 tttatgctat ccatatgttt aattattgga cttacaggtt gtagtttact tgtaggcaag
     2401 aaagataaag agaaaattat agatgatttt aaagttgcag tggtaactca accattatct
     2461 gaaaataaag tacaatacaa tatggtggaa gagatgacaa aagaatatga ggaagagaat
     2521 aaaattgata aagacaaaga tggtcaaact aaagttaaac aaactataaa acatgttgta
     2581 ttgccagaga attttacttc taatatagat agtgctatta ataagatagt aaagttggct
     2641 gacgataaag aagttcaggc tattgtagtt tcaactgacc aagcagggct tttacctgct
     2701 ttacaaaagg taaaggaaaa gcgtccagaa ataataacta tttctgcccc tatgggagat
     2761 gataaaaacc aattaagcca atttgttgat gtgaatctag gggttagtgc agaggaaaga
     2821 ggaaaagtat tagctgaacg ttcaaaagaa atgggagcaa aagcttttat tcattatgcc
     2881 tctacagatg atttgaaaga tgttaatata gccaaaagat tagaaatgat taaagagact
     2941 tgtaaaaata taggattgcc atttgtacaa gttaatactc caaatataaa tactgaagaa
     3001 gacaaaaaca aagttaagca gtttttaaat gaagatattg aaaaacaagt taaaaaatat
     3061 ggaaaagata taaatgtatt tggtgtaaat gagtatatgg atgaagttat attgactaag
     3121 gctttagaat taaagtatat agttgcagag caaagcaatc catcaccaat acaaacgtat
     3181 cctagtgtta tgggtctaaa aatatctgaa aaagatgctc aaaattatga taaaataaac
     3241 gacatgataa gtgaaaaggc taaggctttt ggtatgtcaa atagacttgg tggatatcct
     3301 atgccaatgg atgcttttct accatcagta gcaatttatc ttgctactga aatggtgaaa
     3361 caagatttaa cacaagaaga tgtatgtgac ccagattatt tagaggcgtt tacagaatta
     3421 agatttggaa tcggttcaga atttactcct ttaacagagg tactttataa ttatcaatca
     3481 gtaattttaa gccaattaat ttattaatat taagg
//
LOCUS       DAEPOZ010000293         3505 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.294, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000293 DAEPOZ010000000
VERSION     DAEPOZ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.294"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            167..856
                     /locus_tag="KR440_003410"
     CDS             167..856
                     /locus_tag="KR440_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0700412.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYFVKTAVDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSEDMVIQYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNTIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            856..1773
                     /locus_tag="KR440_003411"
     CDS             856..1773
                     /locus_tag="KR440_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0700413.1"
                     /translation="MELFLVVISIVLFAFYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLLEKFYSRQINYNRSEKAILQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATLEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLANSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            1846..2772
                     /locus_tag="KR440_003412"
     CDS             1846..2772
                     /locus_tag="KR440_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0700414.1"
                     /translation="MNYIIETHQLKKIYKDKAVVNDVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVKPNSGEIVMFSKKVAETDFEILKKIGTIIENPYFYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     KNQIGINSIGRKQSSLEDYFFQTTGTGGIETL"
     gene            2769..3449
                     /locus_tag="KR440_003413"
     CDS             2769..3449
                     /locus_tag="KR440_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0700415.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSILGILLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLVFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
BASE COUNT         1337 a          378 c          587 g         1203 t
ORIGIN      
        1 tatactatat tttaattatt ttttcaataa aacttattga aaagaacttt aacttagaag
       61 agtatcacca aattgcagat acaatatcat aagcagttat ttattgacaa ccctttttaa
      121 taaatatatc atttgaagta tactgacgaa aaggagttga ataataatgg acatcttatt
      181 ggttgaagat gataagaata ttcgagaaat ggtatacgat tttctgatta gtgagaatta
      241 ttttgttaaa actgccgttg acggaaatac agctattgaa atatttaaaa attattcatt
      301 tgatattatt ttactggact tgatgttacc aaagtctagt ggttttgaag tattaaaata
      361 tatacgaagt gtgagtgttg ttcctgttat tattactact gcgaaagata gtgacacaga
      421 taaaatgcta gggcttaatc ttggtgcaga tgattatatt acaaagcctt tttcaatcgt
      481 agaattgctt gcaagaatta aagctaatat tagacgtgca acggtgtata gcagttcaag
      541 aaactctgaa gatatggtta ttcagtataa agatttgatt ataaacatat caaaatatgt
      601 agtttcaaag aatggaattg acttaaactt aacacataca gaatttgaaa ttgttaaatt
      661 actagcgact aatcaaggac gtgctttttc aaaggaacaa ttatacaaca ctatttggaa
      721 agaaccttat tatggaaatg aaaatgtgct aaatactcat attaacaggt taagaaataa
      781 attgtctgat ggtatagaaa aaaataatta tattaaaact ctttggggaa ttggctacaa
      841 aatggaggaa gaataatgga gttattttta gttgtaattt cgattgtact atttgctttc
      901 tacttaaaag aacgaaataa gtcaaagaaa atggaaagtg attataagta tatcaattca
      961 agacttcgtg atataacaaa tgatgatgaa attaattata ttttagtacc atctgatatt
     1021 tcgattgtaa aagaaacagc agtagatata aataaacttc ttgaaaagtt ttacagtagg
     1081 caaattaatt ataatagaag tgaaaaagcg atattgcaaa tatttacaaa catatcacac
     1141 gatttacgaa ctcccatcac agtattaaag ggttatattg aaatgctata tcttcaaagt
     1201 caaaaagaag aattatcttc ttcaatgaga gcaactttgg aaaaaatgca aacaaattca
     1261 aatgagcttg ttaattcaat aaataacctg tttaatatgg caaaaatcca atctggagat
     1321 atgatattaa atcttcaaaa atccaatcta acacaattat gtcatgagat tattttagag
     1381 ttttatgatt tactagataa agaaaacttt aatgttgaag ttaatattga agatagacct
     1441 atatatgcaa atgttgacac agaagcatta caacgtatac tcaaaaattt aattgataat
     1501 gctataaaat atggttcaga tggtagattt ttagggattt cactatatga aaaggaaaaa
     1561 catgttttta tagatattga ggataaaggt tcaggtattt ctgaaactga aaaagagcat
     1621 attttcacaa gagcttatac tgctgacaga aaaaaaggca atggtttagg tttggctatt
     1681 tctcaagggc ttgcaaactc tatgggaggt tcaatttatg taaatagtag acctaatgta
     1741 aaaacagttt ttacggtaaa attgaaaagt tagaaaaaag ttagatttaa aagagagaaa
     1801 agataatttt attttgtata ctgtttttag aaaggaggga gtatgatgaa ctatatcata
     1861 gagacccatc aattaaagaa aatatataaa gataaagctg ttgttaatga tgttaatatt
     1921 catgtgaaaa agggagaaat ttatggattt gttggaccaa atggagcagg taaaagtaca
     1981 gtaatgaaaa tgttgctaaa tctcgttaag ccaaattctg gagaaatagt tatgttcagt
     2041 aagaaagtgg cagaaactga ttttgaaata ttaaaaaaga ttggtactat tattgaaaat
     2101 ccttatttct atgagaattt gactgctaaa cagaatcttg atttacactg tgaatatatg
     2161 ggttattata acaaagaaca tatatcagag gtattggaat gtgtcggatt gtcaaagcaa
     2221 tcaaacaaga aagtttcaaa atattcttta gggatgaaac aacgtcttgc aatagctaga
     2281 gcaattctta ctaaaccaga attattgatt cttgatgaac ctataaatgc actcgaccca
     2341 gaggggataa gagaaatgcg agaattattc cgtaagttaa atacagatta tggaataact
     2401 atatttattt ctagtcatat tctttcagag gtagaacaaa ttgcggacac tattggaatt
     2461 attcaagatg gtaaacttat taaagaaatt tctatgtcag atatacacaa atatcaaaca
     2521 gattatgttg aagtagatgt agataatgtg gaacttgctg gatacttgtt agaaaaagaa
     2581 tttggaataa aagactttaa aattacttcg gaaagttgta ttgaaatata tgatttacga
     2641 aaagatgtaa aagaaatatc caaaatattt atcaaaaatc aaattggtat aaatagcatt
     2701 ggacgtaaac aaagttcttt agaagattac ttcttccaaa ctacaggtac aggaggtata
     2761 gaaacactat gatacattta atgaaactag aattaaaaaa agttgggcta aaaagatatt
     2821 ttttattttc aatactgggg attttgttat gtatgttttt tgtctttact ggattgaatg
     2881 ataaatctac ttcaatatat gattatgatg ttacatttag gacaatcagt ttagttttct
     2941 gtttttacta tattatatta tttgctgtgt tgaatgtggc atatataata aatgaatata
     3001 caaataaaac aatcttgatt ttattctctt atccacttga ccgtaaaaaa cttattttat
     3061 caaagttact tttgattacg actcttatga tatcatcaat gatagttgga tatatctgtt
     3121 gttttacatt cgttgttgtg ttggataagt attttaattt agttattgga gattttcata
     3181 tttcaatttt atcttattgg ataccaggag caattaaaag tattattgta ttttgctcat
     3241 taggtatatg gacattcgtt gttggaatga taagaaaatc aatacctgtt acccttgtca
     3301 gttcaattat ttttatcaat ttaagacaat ttattttagc tggtacaaat acaactcaag
     3361 acagtctgat ttctgttttt attatagttg gaataactgt gattggtata tattatatac
     3421 taaatcataa agttactgaa attgactaga tactgtggat aggaaggtag ttattaattg
     3481 tatcttcttg ggataataat gattt
//
LOCUS       DAEPOZ010000294         3498 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.295, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000294 DAEPOZ010000000
VERSION     DAEPOZ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.295"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..405
                     /locus_tag="KR440_003414"
     CDS             <1..405
                     /locus_tag="KR440_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HBH0700416.1"
                     /translation="ILKKALEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHTHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            392..1273
                     /locus_tag="KR440_003415"
     CDS             392..1273
                     /locus_tag="KR440_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBH0700417.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDASNISDEIKQNVAKEMN
                     LSETAFVSESNVADFKVQFFTPGLEVDLCGHATIGTFHALLEEGRLNTNKSIFYQETK
                     AGVLSVELKEINNENIFMMEQNTPEFENLDNYRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNDITS
                     VKIGGSAYTVLSGEIMY"
     gene            complement(1499..2854)
                     /locus_tag="KR440_003416"
     CDS             complement(1499..2854)
                     /locus_tag="KR440_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBH0700418.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKVEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT         1336 a          528 c          453 g         1181 t
ORIGIN      
        1 atattaaaaa aagcattaga aatgataaaa aatgcaaaac aagaaattat aatttcttgc
       61 ccttatgaag aaattcttga attaaaaaaa gaactcctaa ttgcttacaa aaaaaatatt
      121 aatattgaaa tactactttt agaaaacaaa agtctgaaag gtcttgataa tattcatact
      181 catggaggta ataaaactac acttgaaaat aaaatgcttt acaaaggaat tattttgatt
      241 gtagataatg atgaaatatt gacaggaaac ttatcagaag gcagtgaggc catttcaatt
      301 tggacaaaaa actctaacat tttatatatt ggagttcaat acataaaaca tgaaatttat
      361 atttcagaac aaataaaagg aggttataaa aatgaacaaa attaagatta aacaagttga
      421 tgcttttaca actatacctt ttggtggaaa tcctgctggt gtcgttactg atgcctctaa
      481 catttctgat gaaattaagc agaatgtggc taaagaaatg aatttatcag aaactgcatt
      541 tgtttcagag tcaaatgttg cagattttaa ggtacaattt ttcacaccag gtttagaagt
      601 tgatttatgt ggtcatgcaa caataggtac tttccatgca cttttggaag aaggaaggct
      661 taatactaat aagagtatat tttatcaaga aacaaaagct ggtgtattat ctgtagagct
      721 taaggaaata aataatgaaa atatatttat gatggaacag aatacacctg aatttgaaaa
      781 tttagataat tacagaaaag aacttgcaca aatgataggt gtgcatgaag atagacttct
      841 tgaatatcct attataaagg taaatactgg actttggtgg cttgtatttg gtctaaaatc
      901 tttagatgat ttaaaaaata tctctccaga tttaaataaa atcaaaaaat ttagtggtga
      961 taataatata attggtataa ctccattttg tatagaaact atagataaaa agtgtgacta
     1021 ccatttaaga tctatagcac catatgttgg agttacagaa gaccctgtat gtggaactgg
     1081 gaacgcttgc gtatcatctt atatagtaca tcacaatatt ttagataaaa ctagttttat
     1141 tggtgagcaa ggtaacttta taaacagacc tggaaaggta tatgtaaata taactagaga
     1201 aaacaatgac ataacttctg taaaaattgg aggaagtgct tatactgttt taagtggtga
     1261 aattatgtat taatcccata cctaataaaa acaaataaaa atactagcta actaaagcta
     1321 gtatttttat ttgttatttt tatttaataa tcacactcta acaaatatta taattatata
     1381 tatcataata ttgtatttag aagtaaaatt ttatttttat tattaattag ttataaatca
     1441 atctaataca acataattac agtagattaa tctataaaac ataataatta cttactgatt
     1501 atttatcatc ctcaacaagt ctacttaatg tcttatcaga taagaataat aatatcgctg
     1561 caacaaatag tattactgga attatagtat atactttaat catatcaaat ttaagagtaa
     1621 atgcatatat atatccatat cctttaccag ctataaaagt agcaaatgtc catactccca
     1681 taagaacacc tagaagcttc ttaggtgcat acttacttac aaaagagtta ccaagtggtg
     1741 agaataacat ttcaccaaga cttaaaagaa taccaaacat gattatccat agtatactag
     1801 ctttagcagt ttcaggagct ccaactcctc ttgaaaattc tgcaccaaca agcattaaga
     1861 atgcaagacc taagaatatt aatccaagac ctgtcttttt aaataaactc atatctcctt
     1921 gtggtctact agcaagttta aaccataatg ctccaagaac tggtcctaat actatacata
     1981 caagtgaatt taatgagtca aaccaagcaa gaggaacatc aaatcctcca acattcatgt
     2041 ttacaaatgc tgcaccataa tcataaactg caagataagt tagataccag aatacccaga
     2101 aaattacaga gaagaatgat ataagtataa tagcccaaat tctcttctta tccatgcttg
     2161 taagaggaga cttatctttt tcttcaactt tttcagcaac tacaccttct ttaaaaggtc
     2221 tctttcctgc atttccaagg aatctccatc catatatgaa ccatattgca cctattacac
     2281 aaagtacagc tgctataaag aaacattgag aaaatccaag tacaccattt tttgcaaatg
     2341 ttttaaggta aagaatacca actgctatag taccaataaa agaacctata ttaacaaaag
     2401 aatattgtac tgaaaatgct gtatcaagtt cttcttgact atcaaaaagc tgtccattta
     2461 ccgcagatac gttaccttta aagaatgcag taccaatcga aactaagatt atcattgcat
     2521 ttaccataga aactgtagtt gcaatagaac caagatagta acctaccccc atgataagca
     2581 tacctatagg tatagtatat ctagctccta tccatctatc tgatatatat cccccaatta
     2641 taggtgcaag gtatgtgaat gcaacaaggt tggcttgcat tatagcagct tgtcctttat
     2701 ctattccaag accaccatga actatagctg ttgtaaggaa tatatatatt aagtactttg
     2761 aagcataata tgctgctctt tcaaatgaga aagttgttga gcagatataa aatcccatag
     2821 gaaatttacc cttctttttt actgttgagc tcataatttt aaatcctttc ttaattgtat
     2881 ttttattttt tacgttttgt ccaaattctt tcttttatga aagatataat attattattt
     2941 tgtaaaacaa tttgtaatta ttattaagat aattgcgcat ttatttgtag agtacatatt
     3001 ctcatggaat aaaattaata cacttcttaa tcataaattt aaattatata aattcactat
     3061 tttcagatat agttataaat taatatattc tcatctaata ccatgaaaaa accttcgcca
     3121 tatgcaattc ttatttttta cgtgatagta ttttacaaac taaaatcccc cttttgtagc
     3181 aacttctgta attacatttt agtgcttttt taaaggagtc tcgaataaag caacatatat
     3241 tttgttcaaa taatgttttt atttactttt taaatttata tattacttca ctcaaccaat
     3301 tctcaactta aataaataaa ctactatcta ttcacagatt agacctattt cttacacatt
     3361 agtttataat aaacactgtg aatctaaaca taaaaactgt tattttaaag taattaccca
     3421 tatattcgct ttgatactat ttattgaggt tatactcttt atttataata tattatttat
     3481 aaaatacctc aaaatata
//
LOCUS       DAEPOZ010000295         3488 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.296, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000295 DAEPOZ010000000
VERSION     DAEPOZ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.296"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            278..616
                     /locus_tag="KR440_003417"
     CDS             278..616
                     /locus_tag="KR440_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0700419.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
     gene            638..955
                     /locus_tag="KR440_003418"
     CDS             638..955
                     /locus_tag="KR440_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0700420.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            1105..1782
                     /locus_tag="KR440_003419"
     CDS             1105..1782
                     /locus_tag="KR440_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700421.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(2110..2877)
                     /locus_tag="KR440_003420"
     CDS             complement(2110..2877)
                     /locus_tag="KR440_003420"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBH0700422.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKVAVMPQNERDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
BASE COUNT         1401 a          447 c          405 g         1235 t
ORIGIN      
        1 acatatctaa tatgtaatta caataaaaaa tgctataatt aacatattat taattcgttt
       61 tattattgta gtatggctat gaaattatga aaatatttct ctaattaaaa atatgattta
      121 tccatatttg tttattgtag atacagacta tacaaaaata aatagcactt atttcagtat
      181 aaaaacatat atagtctata tttagactaa aaaagtataa atagtctata taaaacaact
      241 taaactatga atataatcta aagtggaggg ataataattg gcaaatatag gaaaaataat
      301 aggagataga ataaaagtct atagaacaaa attgggatat agtcaagagt ttctagcaga
      361 aaaggcaggt ttacatccaa catatattgg tcagattgaa agagctgaga caaatacatc
      421 tatcaatatt ataatgaaaa ttgctactgc tttagacata ccacttgaat tgttatttgc
      481 aaatattatt acaacagaag gcgttgataa ctctgtacct ttagaatgtt atgaaatgat
      541 taataaatta actgaaaaag aacaaattgc aatgctagag ttaataaagt gcatcattaa
      601 atacaaagaa atataagtga gggaaatagt aacaataatg aattatatag gtaaaagact
      661 taaagaagaa cgaaaaaaag caaatttaac ttcaaaagaa tttgcaaata tggttggagt
      721 atcgccttgg tatataactc aaatcgaaag tggtaaaaag aatccaagtc ttaaaacatt
      781 cataaaattt gtaaatatat taaatatttc tgcagatgtt cttataaaag atattacttc
      841 cactggaaaa acatatttag aaaatgatat aaatgaagag ttaaaagact taaattcaag
      901 agaactaaat ctgatatcac agattataga catcataaaa aagaataaaa tataattctt
      961 tatattttat ataaaaaaaa aggttatttg aaataaataa cctttttttg tctttttgtg
     1021 ttaacattgt cttatagtgc taatgagtac tattagacca tgttctatgg ccttatttta
     1081 atataaaacg attggagata ggctatgatt gtgattgaag gtagtgataa atttaagatt
     1141 gcaaaagaat atattgatgt agaatatact ctttttagca aagtaacttt taggtatgaa
     1201 aagttgaaat ttaaagataa tgctgaattg gaaaaaatta agatgtttaa atataaaaat
     1261 ggctacatcc ctaataaatt aaacctttct tttggatatg gattctcttc ttataaaaag
     1321 caaataatta gagaaactgt agatacttta agattgacag aaattttttc aagcgagaac
     1381 atagaagata ttaaatttat aaaagatggt acaaaaaaat tagaaattag catagagaaa
     1441 gttgtgaaat ttaaacgtcg aaaaaaaaga aattatgttt gttgctattg ccctgatatg
     1501 tatagagata taaaactcga caaagaatct atcaataaga tatacaacag aaaaataaaa
     1561 atagaaagag aagttaatat ttttgaagat gaggatgtta taataaacaa gaaagtattg
     1621 aagtttccaa agtcttggac aaagaatatg caaaaatatt ggttaagtga aaataagtat
     1681 cccatacatt ctactgtaat tgatgatgat agatataaat gttgtaatgt acaatataca
     1741 aaaaatagag tgataatatt atattacata tataaccatt aatatacata attacaaaaa
     1801 aataatgaca aatatacata atacaaatat atcttctact ttagataaaa cagttattat
     1861 gaaaacttac tatctataaa taatagaaat gctagtatac catctataat ctaatgtcta
     1921 gtaggtttta atggcatatt ttaaatatgc catagttgat ttataagatt ttctatatca
     1981 ctttcaaaat acacactaaa tagttgcttt tcagatataa ttgcccaatt taaattttac
     2041 tatctgaaaa gttttttata tttaatttat atcttcaaaa ataccgtcta atatttctat
     2101 ataattaatt taattaatac tcttatgtaa tagattaagt actgaattta actctttaaa
     2161 tgatatttgc cctggtgctg aaacactttt tgcagctcca aaagtcaatg cagaaccaaa
     2221 tatttcacca cacaatctac ttataacacc cattcctgac attgacattg ttattattgg
     2281 tctatctgca taaattttaa acatttcatt tgtagcttca agtagcacta acacatctct
     2341 ttcattttgc ggcataacag ctactttggg taaatctgcg cccaattcct gcattctaca
     2401 taaacgagat acaatctctt ctttttttgg agttttatta aaatcatgat tagatataat
     2461 tactttcact tcttttttat gagcaaaatt aactacttca tctatgacct catctcccat
     2521 aaataactct acatctatta aatctactaa acctgtatta gaaatttctt tattcaaagt
     2581 agtataataa tctcttgata ttaatttttc tcccccttct actacacttc taaatgtgaa
     2641 taataaaggg atatcatgta tataacttct taattcatag agaacttcct taacttcttt
     2701 aatattttct acattctcaa aaaaatcaac tcgccactct ataatatcca aacatgcatc
     2761 ttttaactct ttagcctctt ttataatatc ttttttattt ttcccaataa ttggtacaca
     2821 aattttaggt ctaccttctc caatagtaat attttttact tgaacttttc ttttcatatt
     2881 ctcctccata atcagaaatt ctataggact ttaaattcta ttttctgcat aatttaatta
     2941 tacattaaaa atactataca ttataaaaat actaatttaa aaataatttt tattctaatt
     3001 cttaaaacaa tcccacatat tttagaaaag gtactctata atatacaaac acttcttttt
     3061 tacctaaatt tattttaaca ttctataatt ttcacaatat tctttaaatt ttactcatat
     3121 aaacaatatt attctggatg ctaatagaat taatctataa atcatagtat aattatgcag
     3181 aaataactcg aatttcacta cttataaaga gattgaatat taatgaatct aaatcatttg
     3241 cattacttca gagttttgac taaagttgaa caccatacaa aagtagctac tcaattgttt
     3301 ataccccaac caagcttaag taatgttata tagattcttg aaaaagaatt tggtatcttt
     3361 ttatttgaaa aacaaggtag aaatgtttcc ctcactaaat acggaagttt ttttctaacc
     3421 tatgttgata aagcattagg ttaattagaa aaagtttaca tataaaatat attaaattgt
     3481 tgcaagtt
//
LOCUS       DAEPOZ010000296         3481 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.297, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000296 DAEPOZ010000000
VERSION     DAEPOZ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.297"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..525
                     /locus_tag="KR440_003421"
     CDS             <1..525
                     /locus_tag="KR440_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0700423.1"
                     /translation="CILFLLYSMVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIP
                     TYLAGSYLLKKYSSLNLLKLSAIMITVQFLLFALTNDSNIIIILCVFQVFSTPLILIT
                     SKRLIFEVSPKKLRSSSQLIALSIFMGGSSLIIPTVAGFLSINIGYNYTLMILSLFGC
                     LAYGLIVLLNYLQE"
     gene            843..1595
                     /locus_tag="KR440_003422"
     CDS             843..1595
                     /locus_tag="KR440_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0700424.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFIAIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPAVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            1596..>3481
                     /locus_tag="KR440_003423"
     CDS             1596..>3481
                     /locus_tag="KR440_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0700425.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQEENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSLIVIGYGYY
                     LSTNIRTGDITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKHIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEVKTKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNGYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAIFYTLPMLSATISSYFALRLAGDLLMNDLSGIYILSLVICLIIFVIYGAVS
                     VNKF"
BASE COUNT         1394 a          355 c          550 g         1182 t
ORIGIN      
        1 tgtattttat tcttattata ctcaatggtg gtagcaaata actgtactgt aatagataaa
       61 atgatagctt taaatgctac aaattctcag atatctttaa agtggtcttt acagtcatta
      121 ttagagatac caacatatct ggctggttct tatttattaa aaaaatatag cagtttaaac
      181 ttattaaaat taagtgctat tatgataaca gttcagtttc tattatttgc tttaacaaat
      241 gattcaaata tcattattat actgtgtgtt tttcaagtgt tttctactcc tttaatttta
      301 attacttcaa aaagattaat atttgaagtt tctcctaaaa aacttagaag tagtagccaa
      361 ttaatagctt taagtatatt tatgggaggt tcatctctga ttattccaac tgttgctggt
      421 tttttgagta ttaatattgg gtataattat acattaatga tattaagtct ttttggatgc
      481 ttagcttatg gcttgatagt cttattgaat tatcttcaag aataattgtt ttaatatata
      541 gtagtaatat cacttgttta tagtacctgt atttgccaca atcaaggttg tatttttggt
      601 tttttataga gaagaattat tgtatagatt agaggcttct taggggcttc ttttctttgt
      661 ttatacaaaa aagtgaatca tgcttttatt ttaagatatt tccactataa ttataatagt
      721 cttccatatg ttacaaaagt gtaatgctaa cagttaaaat gtaatattta cttaaggatt
      781 tatttatata aaacctttaa aataaatata tattaaaaaa ttagcaactg gaggctatta
      841 ttatgaaaga aatattaaaa ataaaaaata tatcaaaaga ttatggaata aaaggattta
      901 aaacaaatgt gttaaaaaat atatctctta cagtcaatga aggtgatttt atagcaataa
      961 tgggtccatc tggtgcagga aagacaacat tgctaaattt aatgtctaca cttgacaaac
     1021 aaacttcagg agaaataata ctagatggaa ttaatatatc aaaagtaaaa aataatgaac
     1081 tatcaaaatt aagaagagaa aaaatagggt ttatatttca agactataat ctattagaca
     1141 atatgaaact aatggacaat atagcacttc ctctagcatt aggaaaaaag aaaagcaagg
     1201 agatagaagc taaggtattt tctatagcta aaaaatttgg attagaaaat catttagata
     1261 aatatccata tcagttatca ggaggacaaa aacaaagagg cgcagcagct aggtcattaa
     1321 ttacaaatcc agcagtcata tttgctgatg aaccaacagg tgctttggac tctaaatcag
     1381 catatgaact tttagaatca ttagaaaaaa taaacagaga aaacaacgct actattataa
     1441 tgattacaca tgaccctcta acggcaagtt attcaaatga agtatatatg ataaatgatg
     1501 gaaatataaa gtgtaaatta aacaaaggaa atagtagaaa agaattttat ggaaaaatta
     1561 tggatatgct agcctctatg ggaggtgaga tgtaagtgag tatacttgga attgcttata
     1621 ataattttaa gagtaacata agaacttatc tagcattttt tatatctatg gttttttcag
     1681 tagttgtgct gactaatttt gaattattaa gatatggaga tgctataaat gtgttacaag
     1741 aggaaaataa aaaatttaca ctatcagtgt tagtagctgt aataatgata ctatcagtat
     1801 ttttattttt ctttatatgg tatgcaacaa atatattttt taagaatagg tctaaagaaa
     1861 ttggaatatt gtcctttatg ggattagatt tatatactat aggaaaaata tattttgtag
     1921 aaaatatgct tataggaata agttcttgta taacaggaat aattataggg ataataacat
     1981 ctagattttt tcaagtagtg ataataaaat tatctggatt taatataaag gtatctaatg
     2041 gacttagcat agaagctata ctatatgcta ctttaatctt tatttctata tttataataa
     2101 tggctgtaaa aggttttata acaatatgta gaagtagtgt aataaatcta atcaatatat
     2161 ctaaaaaaca agaaaaaata ccaaaggtag gaataggtat atatatatta gctatagtat
     2221 cacttatagt aataggatat ggatactatc tttctacgaa tataagaact ggagatataa
     2281 ctagtgtact acctgttata ggtataataa tagtaggtac ttatggctta ttcaaatcag
     2341 ttatgcctgt tatatttgat attataatca aaaataaaaa aatattattt aatggaaata
     2401 atataattgc gataaacaat ataaactacc gattaaataa aaactataaa acttatgcta
     2461 taatagctat aattataact acaacaatat caactcttgg tgcttcagtt gctatgaagc
     2521 atatacataa gaatgcccaa gaacaaagaa atgtgtacac tattagcata gtttcaatgg
     2581 ataaagatga agtgaataaa aaacaaatag agaacataat aaaagatact aataagataa
     2641 agtactccgt aaatccagaa ctaacaatct taaagaaaac agatataaaa gaagcttatg
     2701 atacaacaga tactataatg aaatatagtg atttttgtaa tactcttaaa gtgaatggca
     2761 atgataaaga attaaaagag tatggtgaaa gattagttag tggaaataat gtaatagagc
     2821 taaaaagtcc tcaaacgata gcatcaatag ctacaaaaga agtcaagaca aaacttgatg
     2881 gtcagttata taatatttca aggggagaag taaaagttcc agtattaggg acaggactta
     2941 atcaaagtat tatagtggta aatgatgata catattttaa actaagagat aagggtcaac
     3001 aagtatattt ttatggtgca aaggtatttc aagaagaaaa tagccaagta atgttcaaca
     3061 atattgaaaa gaatttaagt ttaaaaaatg gatatattca gaatggatat aaagctcaag
     3121 gtgagagtca atggatgaag tttgcatatg tagtgcttgt gtttttattc ttagtctttg
     3181 caattgtagc tggaagtata atatatatga aaatatatag tgatgcatat gaagataaag
     3241 agaaatacaa gattttatta aaaataggta ctacagaaaa agatataaat agagctatac
     3301 ttaaagaagt agctatattt tatactttgc caatgttaag tgcaactata tctagttatt
     3361 ttgcattgag attagcagga gatttattga tgaacgattt atctggtata tatatactaa
     3421 gtttagtgat atgtttgata atattcgtaa tatatggagc agtatctgtt aataagttta
     3481 a
//
LOCUS       DAEPOZ010000297         3478 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.298, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000297 DAEPOZ010000000
VERSION     DAEPOZ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.298"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..1834
                     /gene="asnB"
                     /locus_tag="KR440_003424"
     CDS             <1..1834
                     /gene="asnB"
                     /locus_tag="KR440_003424"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBH0700426.1"
                     /translation="KKEDIKFKIKESVNNTRLSSRIEYKDEEKTFDFERFNVIDAIYG
                     NKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLLASYIEFGKNCVHKI
                     KGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYKFLLEYMKNLNINER
                     SLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNKLEFRSSTKFFYTKN
                     HLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIITTVASQINPNIKSFS
                     AGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIYSLDDPIADPSEVGL
                     YFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPTYIKSILGRVSELMP
                     NIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIYEYLLSNLYRDAQKN
                     NYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKDVFDVAKNLRMEQKI
                     NKNNTKVLLREAFREIVPEYVVQRKKLGFPTPIRVWLKDSLGDIVKETIYNSNVDEFI
                     DKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY"
     gene            complement(1926..3362)
                     /locus_tag="KR440_003425"
     CDS             complement(1926..3362)
                     /locus_tag="KR440_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBH0700427.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKSSSYIKEVHFTRY
                     IGVDDSIDKYIEKVKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYDSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWM
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIAQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESNLVKIFANNFIEIVN"
BASE COUNT         1354 a          395 c          521 g         1208 t
ORIGIN      
        1 taaaaaagaa gatattaaat ttaaaataaa agaatctgta aataatacaa gactttctag
       61 tagaatagag tataaggatg aggaaaaaac ttttgatttt gaaagattta atgtaattga
      121 tgctatttat ggaaataaac catttgaaaa agatggaaaa gttattgttt ttaatggaaa
      181 tatatataac tatcatgaaa taaaagaaga attaattaaa aaaggacaca attttactac
      241 aaatgatgat attgaagtct tattggcatc ttatatagaa tttggtaaga attgtgtaca
      301 taagattaaa ggaatgttca actttataat ctatgataga gaaaatgaaa gtatatttgg
      361 agcaagagat ttatttggga taaaaccact ttattatata aataaggaaa atgcaataat
      421 tttttcctca gagtataagt ttttacttga atatatgaaa aatttaaata taaatgaaag
      481 aagcttacaa agttattttt catttcaata tgtacttcct gaagatacta tgatacaagg
      541 aataaggctg ataccagcag gtcactattt tagggtagaa aatggtatac tatctttaaa
      601 gagatataat aaacttgagt ttagaagtag tacaaaattt ttttatacta aaaatcactt
      661 aggcaataga gatgtgaatg aggatgatat aagaaatata gtagtagatt caatagtaac
      721 tcatatggaa gaggagaaag aaattggaac atttttgtct ggtggaatag attcatctat
      781 tataactact gtggcttcac aaattaatcc aaatataaaa tctttttctg caggctttag
      841 tgtcaaagga tatagtgaat tagaagtagc aaagaaaaca gctgataagt taggtataga
      901 aaatatccag attaatataa cacaagatga atatataaaa tcattaccga atgtaattta
      961 ttctttggat gaccctatag cagacccttc agaagtaggt ctttatttct taattaaaga
     1021 ggcaggtaaa catgttaaag tagcattgtc tggagaagga gcagatgaat tatttggagg
     1081 atataatata tataaagagt atagtactat gaaatcagtt gtcaattcac ctacttatat
     1141 taaaagtata ttaggaagag tttcagaact tatgccaaac ataaaaggaa gaaattatct
     1201 ttatagagct acaacaccat tagaaaaaag atatatagga aatgcaaaag tttttgaaaa
     1261 cagcgaagta aaaagattct tttttaagta taaggaaaag aatatatatg aatacctgtt
     1321 gtctaatttg tatagagatg cacaaaaaaa taattacgat tatataagta aaatgcaaca
     1381 tatagatgtg aatacatggc ttcaaggcga tatacttcaa aaaattagca aactttctac
     1441 agctgagcag gttgaactta gagtaccatt tttatataaa gatgtatttg atgtagcaaa
     1501 aaatttaaga atggagcaaa aaataaataa gaataataca aaagttttac ttagagaggc
     1561 gtttagagaa atagtcccag aatatgttgt tcaaaggaaa aaattaggat ttccaactcc
     1621 aataagagtt tggttaaaag attctctagg ggatattgta aaggaaacta tttataattc
     1681 aaatgtagat gaatttatag ataaaaaata tgcaataaaa cttttagata cgcatcttaa
     1741 aggtcataga gataattcga gaaaaatatg gactatattt acattttgtc tatggcatca
     1801 attatttatt gagcataaaa atgtcgagta ttaatacttt tttcaatatt aatcagaggg
     1861 caaaagcatg caatgttttc accctcttga ttattatgga ataggataag tagaattttg
     1921 attgattagt ttactatctc aataaaatta ttggcaaata ttttgacaag attactttca
     1981 acagctaaat ttaagttttg tccatcatca tatccagcgg aaatcaatcc tagtaagttt
     2041 ccaaacatat ctaaaagagc tcctcctgaa ctaccactag agattggagc tgtaaattgt
     2101 atcatttgaa ccgtttcaaa atcacgaaaa gctgagataa ttccatctga aactgtatta
     2161 aaaagaccta aagggcttcc aattgcaact attttttgac ctctcacagg ttttttactg
     2221 actttaacag gtattggttt gcattttcta tcaaccttta tcacagctaa atcataatct
     2281 gaatggtatt ttataatttg gtaagatgta taaactttat catcattttc aaatctaact
     2341 aaaaatgaat acccatcatt gactacatga agattcgtaa gtatataacc ttcattattt
     2401 attactactc cagaaccact cttaaacggt ttattttcgt tattgcacac aacaatcata
     2461 acaacagaag ctgataactt tgcaaggtct tcataagaaa gttctacttc ctcatcttgt
     2521 gcaatttttt tagatgttag atttgtgtta ttaagtgtat ttttagagtt aggtttattc
     2581 gtagtaggag aagtcttatt ggtattttca ctgtaattta tatttgtaat atgcctttca
     2641 aaaggtattt ctaatatatt tggagttttc ctagaaagtt caaatagagt agaaaatttt
     2701 gaacattcag gatataaatc aagaatatct ttatttggat ttatatataa aatatcacac
     2761 ccaagttgtg ataaaaaata taagaaaaaa agttcttgcc ttttaatatt tccaaagtat
     2821 actatctttg gtgaaatagt agtcttcacc ttgttataaa ataatttagg tagaaaatag
     2881 tccatccaac acaaaagttt aactgcaaag tttttgatga tagaggatgt acaatttggc
     2941 atacttgtgg aaaataaatc taaagtattt ttaaatgcaa tctttttagt ccaatttaaa
     3001 gtgtcatttt ctattttaca tggaaagtcg atattatata aatcccaact gttaaactta
     3061 gaactatcat aaatattaaa aattttaagc atcttatctg tttgttcttt gtcaaaattt
     3121 atttttagat tgtttaatct aatataggga attgtaggat tattaaaaaa ataagtatct
     3181 atatttttaa ctttttctat gtatttatca attgaatcat caacacctat atatcttgta
     3241 aagtggactt ctttaatata tgaagaactc ttatttttat aggtagtatc agatataaat
     3301 tcaattagag gttcagttga cttttttatt gaaatcttta atggtttaat agtacaaatc
     3361 atattttttc tcctataata agatgatata atctattata aaacaactta aaacaatata
     3421 gtacaataat tttgtagttt caaaataaaa tatgtacttg tttatataaa tgatatat
//
LOCUS       DAEPOZ010000298         3458 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.299, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000298 DAEPOZ010000000
VERSION     DAEPOZ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.299"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(61..2142)
                     /locus_tag="KR440_003426"
     CDS             complement(61..2142)
                     /locus_tag="KR440_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBH0700428.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASSFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSTKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGTNINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKPKKEVKDSSWNMSINKTTKVFLMISFITIIVVMIL
                     SMLYIKKYKRR"
     gene            complement(2165..3421)
                     /locus_tag="KR440_003427"
     CDS             complement(2165..3421)
                     /locus_tag="KR440_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBH0700429.1"
                     /translation="MRDLNVSEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYKVSKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIEGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTTVIISDILCVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
BASE COUNT         1161 a          549 c          313 g         1435 t
ORIGIN      
        1 aaatttattc atatatttcc tcaaattata tacctctttt aaaataataa atttatcata
       61 ttatcttctt ttatattttt ttatatatag cattgataat atcatgacta caattatagt
      121 aataaaagaa atcattaaaa atactttagt tgtcttattt atactcatat tccagcttga
      181 atcttttact tctttcttag gttttaaatt ataagctaaa tcttctacct ctccgtattc
      241 atcaattatc aatgtatctc cttttaaatc attaacttta cttttattca acaaataatc
      301 cattcctaaa tataagtttt tttcattcat tgaactaatt accatgatat ttttttgatt
      361 attatatggt gattttatta attgaatggt agaaatattt tttccataat catcaataaa
      421 gcttatctta tcatttgata taaaattgga gtaattttta tcgaacttta tatttaaatt
      481 attatttaac atctttataa cagaattatt atgtggcact ccaaaaacta caatattagt
      541 atccttatac ttgtcactaa attcttttcc tctaattaca tttatatttc cattatgtga
      601 atttatattc gtacctaaag taacacctaa tctaaacatc caggtcatag cttgtgaccc
      661 tgaataatct ggcattatca cagttaaatc attaaactca tcatctctaa caaatggata
      721 cggataattt tcaaaagata aggtttcatt ttctttcgta gataacgcca aataagaatt
      781 atttgataca tatgcccaag gattattact ctctctagta acacaattcg aattttttat
      841 actcaaatta aatgtcaatt taacttgata atagttttta ttatctatat ctttaggtat
      901 ttttaattct aacttatcat tattagaatg tgacctatcc aattttttac ttcccacaat
      961 aacatcattt atagacactg taactaaaga tttttcaaaa tctaaattgt cagaatatct
     1021 taaatttaag ataatctttg aaccatcatc taatacctta cccgttggta ttttgacatc
     1081 aaacaaagct tgttgattaa atgctccttc caacaaaaag tcagaatatc ctaagtcttt
     1141 taatgttaaa tgacctaaat ttaatttttg ttccctatta atttttatat tagtctcttt
     1201 gtttacaagc actgatgatg ataaaacttg gttagaaagt ctattttcta ttaataaatt
     1261 tgatgccttt attaaatcat cttcattact accaattacc accatcatct tcttattctt
     1321 attgtatggt gaatctactt gcttgattat acagtttgaa gataataggg tttgttcctt
     1381 tatacttaaa atatccaaaa tttcttctgc tgtattttca ggtttaccaa tatatataat
     1441 attatcatct gaccaatttt tcatttctga atataattta acatctaact ttaaatcatc
     1501 attttttgtt atttttccaa atgatgatgc caaattaaac actgctgtag tctctcctct
     1561 agtcatatta tctggtacta ctatagttgt atctaattta tgattatttt caatttctgc
     1621 atatggatac ggatattcat ttattgagtt ttccaccttt ttctgcttat atctaattga
     1681 tatatatgat tctttgtgaa taaccatcca gttcccagta ttggaatcat cttggcaaat
     1741 tttatcagat attgttttgt atgtttttat ttttatttca ttatatcctt gaattatata
     1801 atcttttgga actaatactt ctaatgtatt tttataattt gtcttagcat ttaatttaat
     1861 agattttatt ggtacattat ttatcaatac agttaaactt gaaacatctc catttaatat
     1921 ttggctctta ctaaaattaa gatgcaataa aacttcttca atatcccaat ttttatttac
     1981 ttcaaaaaaa gtactattac ttcctataac accatcaata gttatatctc tttcaaactt
     2041 ataattttta actttagtct catcagcatt tactttgtat ataagtgata tatttgaaaa
     2101 aaacaatact aaagatataa ttgatataat aaattttttc atgttaattc ctcctgtaca
     2161 tgaatcaaaa tctttctgtt ttataccatt taacctctct tttaaaaatt atatctttta
     2221 gatactctat catccctttt atagctacaa acagccatag ttgagagtat gtaaaataca
     2281 ttattgcaac tatgaatata ttttcaattg ttgcttcacc tttttctata gtaaggctta
     2341 tactcacttc tataataaat aaaagataag atagtatcca tattagaaaa aagttaattg
     2401 gaatagaaat ttctactagt ttagatatac ttaaaacaca taaaatatcg gatataatta
     2461 cagttgttaa aaatagaaaa tatacagaaa aaaagtaagc tatatcaaac acaatcttat
     2521 ttcttccctg tttaaatata ttttttatat atttcattaa tacatatata ttcccttttg
     2581 cccatctagt acgttgcttt atccacacct taacagtttc tggctcttgt tcccatgtta
     2641 tagattgtgg tacaagcttt atcttgtatc ccaatttata tatcctaaaa cttatttctg
     2701 tatcttctgc aattgcttta ctatcccatc cttcaatttc ttctatgata cttctcctca
     2761 atataaagtt agttcctggt attgtacata gattaaaaag ttgccatcta ccagcttgtg
     2821 acatccattg aaagcttaat gtttctatgt ttataaattt ggttaatagg tttttatttt
     2881 tatttcgagt cctaaatttc ccaataacag cacctaattc atcattcatt actatagttt
     2941 gtactagata tctaagtgca tttttatctg gtgtgttatc tgcatcataa acagctataa
     3001 aatctccttt tgaaacctta tatccaatat ttaatgcatt agactttcct tttccacctg
     3061 ttaaactatc tgtatttatt atagtgaaat tataattgtt gtacctatcc tttatatttt
     3121 ctaaaatttc tttactatta tcagaagaat tgtcatttat aacaataagt tccatcttac
     3181 tcttaggata attgagtaat aacaaagact ccacagttct acctatcact ttagcttcat
     3241 tatgagcagg aacaagtatt gatatcatag gatattcatc aatttctttt atttctttat
     3301 caaagttttt aaaataaaat atatatccac ccatagctaa aataatattt ataagcaata
     3361 aagaccatat actaaacaga ctaaatacga acaaatactc tgatacattt aaatctctca
     3421 taaatttctc ctaaaatatt ttaaaatatt gattctaa
//
LOCUS       DAEPOZ010000299         3453 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.300, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000299 DAEPOZ010000000
VERSION     DAEPOZ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.300"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            116..1039
                     /locus_tag="KR440_003428"
     CDS             116..1039
                     /locus_tag="KR440_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HBH0700430.1"
                     /translation="MGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESINLTKTKIY
                     SNLRDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSKKAG
                     ALIYSIHPIFAFSNKNTNIKKMKDICFSIEGDNEQDDLVIQNFIDGLGNQFFIRDKDT
                     SSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNGFTK
                     ALTGPVSRGDITPIRKHLSVLNKKDEDIYKILSLNLLKIIALGNDNSLKGTKNITIEN
                     AIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            1014..1841
                     /gene="panB"
                     /locus_tag="KR440_003429"
     CDS             1014..1841
                     /gene="panB"
                     /locus_tag="KR440_003429"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBH0700431.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGAKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIADMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGVFGDFVPKFVKQYANIGDIMKDSIKNYILEVNTGAFPQEKHSFSINESELEKLY"
     gene            1860..2708
                     /locus_tag="KR440_003430"
     CDS             1860..2708
                     /locus_tag="KR440_003430"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBH0700432.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNINTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PKNIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
     gene            3119..3301
                     /locus_tag="KR440_003431"
     CDS             3119..3301
                     /locus_tag="KR440_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700433.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
BASE COUNT         1367 a          411 c          526 g         1149 t
ORIGIN      
        1 tatactttaa atgtttttaa ttattttgtc ttatgtaact catatcctca ggaatgagat
       61 tagaaattaa gatgttgtat cacaaagtgg aggtgatact attaaaatag gctttattgg
      121 ttctggaaaa gttggaacat ctttggcaaa ttatttttta tcaaaaggtt attgtgtttc
      181 aggcttttat ggaaggaatc aattatcttt attagaatca ataaatttaa caaaaacaaa
      241 gatttacagc aatttgagag atataattta tgaaaacgat atattattta ttacaacatc
      301 agatgattct attgaaatta tagacaaaaa actttccaaa tttaatttaa aacataaata
      361 catttgccac acaagtggct ctttaaaatc tagtatattg ttctgttcaa aaaaagcagg
      421 tgcattaatt tattctatcc atcctatatt tgcattttcc aataaaaata ccaacataaa
      481 aaaaatgaaa gacatttgtt tttccataga aggtgataat gaacaagatg atttagttat
      541 tcaaaatttc atagatggtt tgggaaacca attttttata agagataagg atacttcatc
      601 cacttatcat ctagctaatg ttttagtttc aaatttagta ttatctttac tagatatagg
      661 agtaagttac tttattaaat taggtttatc tgaagaggaa tctttaaaag ctatcagtcc
      721 tttagtaaaa aaaaatatag aaaatatact tgataatggc tttactaaag ctcttactgg
      781 tcctgtttct cgtggagata taacaccaat aaggaaacat ctatctgtat tgaataaaaa
      841 agatgaggat atctacaaaa tactatcctt aaacctacta aaaataattg ccttaggaaa
      901 tgacaattca ttaaaaggta caaaaaatat tactatagaa aatgcaatcg aaaatttaat
      961 aagtaaatct gaaaaataca aagaaattta caaactttta ggaggaaaaa acgatgaaaa
     1021 ataccataca gacttttaag aacgctaaat atgaaggaaa aaaactatct atgcttactg
     1081 cttatgacta ctcaattgca aaaataatgg atgaatgtga tataaatggt attttaatag
     1141 gcgattcatt aggaatggtc ataaaaggtg aagaaaacac tctatctgtt actatagacg
     1201 aaattatata tcatacaaaa gctgtaaaaa atggtgcaaa aaatgcctta atcgtaagtg
     1261 atatgccttt tctatcttac catgtttcta tagaagatgc agttaaaaac gctggtagac
     1321 ttataaaaga aggaggagct catgctgtaa agttagaagg tggctcaaac gttataaagc
     1381 aaatagaaag cattgtaaat gctcagatac ctgtaatggg gcatttagga ttaactcctc
     1441 aatcagtaaa ttcatttggt gggtttaagg ttcaaggaaa taccagtgaa acagcaagac
     1501 aacttattga agatgctaaa ttaatagaaa aagcaggtgc attctctatt gtccttgaag
     1561 gagttcctac gaaaatagct gatatggtaa ctaattctat ttcaattcct acaataggta
     1621 taggggcagg aattaactgc gatggacaaa ttttagttta tcaagatatg ttgggagtgt
     1681 ttggagattt tgttcctaag tttgtaaagc agtatgcaaa tataggagat ataatgaaag
     1741 attcaattaa aaattacatc ctagaagtta atacaggtgc atttccacaa gaaaaacaca
     1801 gcttttcaat aaatgaatct gagttagaaa aattatacta atattaagga tggtacaaaa
     1861 tgcttgtaaa agaaattaaa ttactaagaa atataatcaa agattggaga aaacatggct
     1921 attctatagg gcttgttaca acaatgggat ttttacatga aggacaccaa agtcttataa
     1981 aaaaagctgt aaaagaaaat gataaagtag tagtgagtgt atttgtaaat ccaactcaat
     2041 ttggaccaaa tgaagatttt aatagctatc caagagatat agataaagat tttaaatatt
     2101 gtatggatag tggagcaaca gtagtcttta acccatcacc agaagaaatg tatttaaaag
     2161 gtaattgtac taccattaat gtttctggac ttacagattt tctttgtgga gctaaaagac
     2221 ctgttcattt tggaggtgtc tgcctagttg tttcaaagtt tctaaatata gtaacacctg
     2281 ataaagctta ttttggagag aaggatgccc aacaattggc tgtaattaaa agaatggtca
     2341 aggatttaaa cataaataca gaaataatag gctgtcctat aattcgtgaa aatgatggat
     2401 tagcaaaaag ttcgagaaat acatatctct ctgaggaaga acgaaaatct gctctaatat
     2461 taaataagag cttaagttta gcaaaagaag aattagtaaa aggcaatcta aatccaaaga
     2521 atataaaaga attaataaca gctaaaataa attctgaaca cttagcaaaa atagactatg
     2581 ttgaaattgt agatagtgag acattacaac ctgttaagca aatagaacat tctatccttg
     2641 tggcaattgc tgtgtttata ggaaaaacca gactaattga taattttaca tttgagttaa
     2701 atatatagta tataatttta tataatatat ctaaaaaata atagaatacc taaaaagtaa
     2761 taaattacct aaaaaataat aaattgctta gaaaataata gattacaata actaaaattt
     2821 taaaattaaa ttccttaaaa gtatgattaa ttataattat ataagtatat tcttattaca
     2881 cttatattaa tttatattaa atatctacta actcaaatat tcataattaa actttaaact
     2941 ggataaattt tcttttaaga atgaaagttt atccagtttt gcgattataa taaccagttt
     3001 tacatatcca cttgtaaaat atatctaagc aatatatcat actatataga tttatgagtg
     3061 aaataatatc tgctatttac tgaatatttt tatttttcgt tttttagggg gattagttgt
     3121 gtttaaagat aagattgatg aatgtgttca tataatgact gcatatattg ctagtttaaa
     3181 agaatactat tcattcatag aaactcaaat tggtgatttt attaaaaaat atggagaaga
     3241 tgttgtagag ttatgtctcc atagagtgat gattctacta tgcgaatgtg gtttggcata
     3301 aaattaaatt tattaaaatg ggtaagaaga atcctatata atattcttct tacccatttt
     3361 aatatatcaa tttcatagct tatcgtttaa aatattttac ctccattact agacaacttt
     3421 acattctctt tttaattcta gtatatttaa atc
//
LOCUS       DAEPOZ010000300         3427 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.301, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000300 DAEPOZ010000000
VERSION     DAEPOZ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.301"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            259..780
                     /locus_tag="KR440_003432"
     CDS             259..780
                     /locus_tag="KR440_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0700434.1"
                     /translation="MKFERLRTSENKLYYKAMELYKISFPFHEQRKSYLQTEILKNKE
                     YQFNLIYDKNQFVGIILCWETDNFIYVEHFCIFPEMRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHEKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            complement(1200..2414)
                     /locus_tag="KR440_003433"
     CDS             complement(1200..2414)
                     /locus_tag="KR440_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0700435.1"
                     /translation="MQIKIGKVIQRLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEKVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNIFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLWYANLSLEIKKMLSEDKGAFFSMYVDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHESLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(2879..3340)
                     /locus_tag="KR440_003434"
     CDS             complement(2879..3340)
                     /locus_tag="KR440_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0700436.1"
                     /translation="MQNSVKQEPCDFSHGRFRFGGKIIRRNEKTKFDWLSRDKEQVEK
                     YINDPFCGVVCSCGFFYDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGV
                     LRLRDRYIKLGVKDVTCKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
BASE COUNT         1207 a          536 c          335 g         1349 t
ORIGIN      
        1 aacttttttg taacttctaa aattattaag attactttta gacctagaaa agtccataaa
       61 tatactgttt ttagattatc aaagcttaat ataatagttt ttagttatgt cttaagcaat
      121 tatctcttag tctatgagat ataagcataa ataaaagaaa gaaatgacac tcaacttaaa
      181 acacattgta tgcaaatact gactatggta aaatgaactc ataaataaat tggaattttc
      241 gtgattagga ggagtaaagt gaaatttgaa cggttaagaa ccagtgaaaa taaattatat
      301 tacaaggcta tggaattata taaaattagt ttcccatttc atgaacaaag aaaatcttat
      361 ttgcaaacag aaattttgaa aaataaagaa tatcaattca atttaattta tgataaaaac
      421 cagtttgtcg gcataatttt atgttgggaa acagataact ttatctatgt agaacatttc
      481 tgcatttttc cagaaatgcg caataataga tatggacaaa gagcgttaga gcttttaaat
      541 aagaaaggaa aaactattat tttagaaatt gaccttccaa ttgatgagat ttcaatttat
      601 cgcaaggcgt tctatgaaag agtaaaatat aagacaaata attatgaaca cattcatcca
      661 ccataccatg aaaaattcaa tgggcataac ctgattgtaa tgtcttaccc agaaaagtta
      721 tctaaaatgg aatatgataa atttaatcac tatctaagaa ataatgtcat gagtttctaa
      781 atataattcc tatttattga actgtataaa acttcataat taaataaaag agatgtctca
      841 aaatagaaat aagactcttt tccattgttt aaataataaa gatatattgg atttcataca
      901 aataaggatg catcatgaac taaaaaattc atgatacatc ctctttttca cgcttaaagt
      961 taagaaaaca tgtgagattt tatagacaaa attacatttt atataacaaa ctttattcta
     1021 tttttataaa tttaggtttc tatttttgtg taataataca gataattcag atttctattt
     1081 tgaaacctct atttttctaa ataacggttt aagaccaaca aaaaaggtga ttcaaatttt
     1141 tattttgaaa cactttcttt taatttgatt aatctttaag tcctatatta aatttttact
     1201 tattctttat ttttcaacat tttttccata tctaaaatct tttcaattac atccttaaaa
     1261 attggttctt ctctaagctc attataactg tcatcattaa gtacaattaa caagtttgta
     1321 tatatattaa aagtatataa atttttagac tctaccttat caaaaaatct catttgactt
     1381 acatccaaac tttcatgtat aggatatttt tctaatattt cttttagact atataaggct
     1441 ttttttgctt cttctttcat gttaaaaaat aaaaaaatta gagataattc catataatcc
     1501 acatacatac taaaaaatgc acctttatct tcactgagca ttttttttat ttctaaagaa
     1561 agatttgcat accacagcaa cttatttttt atatactcca tttctctctc aattgcattt
     1621 ttattaaaat ttctattctc caattttttc tttaattcct tgtattgatt tttgtataca
     1681 ttaattattc ccttacaatc catgcttata ttaaatatat tactaaacaa tttattttga
     1741 agcaatattt ctgcttcttc aagtctattt tgctttaaat atatcatagc taaattaaca
     1801 cttgtatccc ctattgactt atgtatcata tttaaataca actctgcctt ttcataatct
     1861 tccaacatac aatatgcgtt tgataatata ataagagctg tttcttttat ttggatatca
     1921 tctgtatttc tcactatatc ttcaagaata ttcgttgttt cttttactct gtcattaagt
     1981 tcactcttac caaatttata tgaatacatc tgatacagcg aacatatcct tagcttcaat
     2041 aaatagcttt taggatactt ctctatatac atattagaaa gcttttttac agattctaag
     2101 tcttgtctaa attcttcttt tggttcttct gttgataaat caatttcaat tctagcaaag
     2161 cctgattcta attctttata aattttcatt actttttctt cacttaaatc aatcttgtaa
     2221 tttaaaagct tatcaataga cacattaaaa aagtctgcta ataaaggcaa taattctata
     2281 tctgggtaag aatttccact ctcccactta gatactgctg gtgttgacac acctataaac
     2341 tttgctaatt gttcttgagt taaatttcgt tcttttctaa gacgctgtat gactttacct
     2401 attttaattt gcatatatac ctccctagta catctgttgt aatttaagta tataaaaaaa
     2461 atatttacta gtaaatcgag ggaaagttaa caaaatgtct gtaatttaac cattggttat
     2521 attttaatct aaaaccctat ctcattggct tgaaatataa aatctattaa ttaagtttaa
     2581 gtataaccta attaatagat tcacattaaa tagcatttaa atatttagat tttctcttta
     2641 ctacaactta ctatatttaa actttcaagg aattttaaat aaaataaatt ccatatacaa
     2701 tgataataat actgagtgtt aataatttat ttataataac acccacctat tcaataaatg
     2761 gcattatagc aaaaaatcac ttctaggctc tctcttttct actttgcata tacataatcc
     2821 acttttcaaa tattatatat aaataaattt aaagcaaata tatcattata tttttttatt
     2881 acaatatttc tattttattg tttaaccaac aaattatatc ctcaaaaact tcttctctat
     2941 ttatttcatt caacaattca tgccttccat ctttgtaaag tttgcaagtt acatctttta
     3001 ctcctaattt tatatatcta tcccttagtc ttaagactcc tttaccattt tttcctatgg
     3061 ggtctttatc tccagatatt atgtaaatag gtatatcaag tggcactttt tttaagtttt
     3121 ctttatcctc aatttctttt aacccttgaa ctaaatcata aaaaaagcca caactacaca
     3181 ctactccaca aaatgggtca tttatatatt tttcaacttg ttctttatct ctacttaacc
     3241 agtcaaactt agtcttttca tttcttctta ttattttgcc accaaatctg aacctcccat
     3301 gactaaagtc acagggttcc tgcttcacag aattttgcat actaaaatat gtcttactaa
     3361 ttctccacag gctatccccg tagttcctac ggttcttaca taagttattt attttgaatt
     3421 gttatta
//
LOCUS       DAEPOZ010000301         3393 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.302, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000301 DAEPOZ010000000
VERSION     DAEPOZ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.302"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..1123
                     /locus_tag="KR440_003435"
     CDS             <1..1123
                     /locus_tag="KR440_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0700437.1"
                     /translation="KIGFKLIEDEVIYISNYSGVIAYKGTQGRKNTSEIEERISSVIS
                     SALQDIFLISGSDYTKDDEFMVAISDHIKRFLNRARANVKSNNPLLHQIKEKFPIAFN
                     LAVFLSNKLETEFNLKLDEDELGYIAIHFAASNERMKKNTSKKICIVCHYGIGTGQLI
                     SEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIVSTVELKGYEKPVLYIENIFDDSLI
                     ENVNKAFYEKEERRKIISNMFDEKAFFSIKASTKEEVIISLSNKLKERDFIEESSIKS
                     IIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVGILENPIIWDKQEVQLVFMVFFNTK
                     EKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLMALIGN"
     gene            1138..1593
                     /locus_tag="KR440_003436"
     CDS             1138..1593
                     /locus_tag="KR440_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0700438.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            1652..1969
                     /locus_tag="KR440_003437"
     CDS             1652..1969
                     /locus_tag="KR440_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBH0700439.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            1994..3256
                     /locus_tag="KR440_003438"
     CDS             1994..3256
                     /locus_tag="KR440_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBH0700440.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFIDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
BASE COUNT         1339 a          369 c          635 g         1050 t
ORIGIN      
        1 taaaattgga tttaaactta ttgaagatga agtaatttat atttcaaatt attctggtgt
       61 aatagcttat aaaggtacac aaggaagaaa aaacacaagt gaaattgagg aacgaataag
      121 tagtgttata agtagtgcac ttcaagatat attccttatt tcaggtagtg attatacaaa
      181 agatgatgag tttatggttg caatctctga ccatataaag agatttttaa atagagctag
      241 agctaatgta aaatcaaata atcctttact tcatcaaata aaagagaagt ttcctatagc
      301 ttttaattta gccgtgtttt tatcaaataa actagaaact gagtttaatt taaaacttga
      361 tgaagatgag cttggatata tagctatcca ctttgctgct tcaaatgaaa gaatgaaaaa
      421 aaatactagt aagaaaattt gtatagtttg tcattatggg ataggaacag gacaacttat
      481 ttctgaaaag ctaaaacaga atataagtga tttaagtgtt gtaggtgttt atccagttag
      541 atatcttgat atggctataa gtcaagatgt tgatttgata gtatctaccg ttgaattaaa
      601 aggatatgaa aaacctgtct tatatataga aaatatattt gatgatagct taattgaaaa
      661 cgtaaacaaa gccttttatg aaaaggaaga aagaagaaag ataataagta acatgtttga
      721 tgaaaaagct tttttcagta taaaggctag tactaaagaa gaggtcataa tttcactttc
      781 aaataaacta aaagaaagag attttataga ggaaagttct ataaaaagta taatagatag
      841 agaaaatatt tcatcaactg aaataggaaa tctagtagca atacctcaca caatagtgaa
      901 gggagataaa aaatctataa taggagttgg aatacttgaa aatccaataa tttgggataa
      961 gcaagaggtt cagctagtat ttatggtatt ttttaatact aaagaaaagc ataatttctc
     1021 gatatttaaa tacctttata attttataaa agatgaagga ggagtaagag gaattattaa
     1081 aatatgtgac tttaataaat taatggcact gataggtaat taaggaggaa attaataatg
     1141 ggaacaaaca tatttaacaa agaatatgta tttttaaatg ttgatgctaa gtcaaaggtt
     1201 gaagttttaa aatttatatc aaaaaaagct aaagacttaa atttggcaga agatgagagc
     1261 ttagtatatg aaggtttaat ggctagagaa gaacaattta ctacaaattt aggagaatct
     1321 atagcaatac ctcacactaa aaatgatgca attgaaaatc cagcagtagt agtattgaag
     1381 tttaatgaag atgttgtttg gaatgaaggt gaggataagg ttaaattagc aataagctta
     1441 cttatgccag gaaaaagtaa agagaatatt catcttaaat tattatcatc tctttcaaga
     1501 aaattaataa ataaagaatt taaagattct cttttaaaaa gtgataatgt tgaagaaata
     1561 tctaacttaa taaatgaagc tttaggttta taattaaata ctaaggttat aaaataaaaa
     1621 aataattaac taaaaatagg aggaatttga aatgaataaa aaattagtag cattatgtgc
     1681 ttgcccaatg ggactagctc acactttcat ggcagcagaa gcaatagagc aagcagcaaa
     1741 ggcattaggt tatgaagcaa aagttgaaac tcaaggtgct gatggtgtac aaaatgagtt
     1801 aacaagagat gatatattag gagcaactat gataatacat gcagtagcaa taacaccaga
     1861 aggtatggaa agatttgatg gttgtgaagt ttatgaagtt gaattacaag aagctataaa
     1921 aaatgcagaa ggtgttataa aagaaataga agaagattta ggaatataat aaatttattg
     1981 tggaggaaat agaatggcaa ttaagaaaaa agttataaac agttcaggtg cttctacaac
     2041 aggaggaaat aatgtaggaa aatctactcc atctagtcca actcaatcaa agggaaatgg
     2101 aaaatggaaa gaaataagta aacatataat gacaggtata tcatatatga taccagtttt
     2161 agtaatgggt ggacttatag gggccttatc tcaattaata ccatatgcta tattaggact
     2221 tgacccatct gtaggtatag ttgatgctat gaactcagga gaatttacag ggttcaagct
     2281 tagtctttta aatatagcac aattaatgtc aaatttcgga tttactttgt ttggatttgc
     2341 gataccactg tttgcagcat tttgtgcaaa ctctataggt ggaaaaacag ctttaattgc
     2401 aggatttata ggtggatata tagctaataa acctgtaggt gtaccacaat tcattgatgg
     2461 acaatggact gaagttgttc cagttgcatc aggattttta ggtgcaatac taatagcatt
     2521 cataatagga tattttgtaa aatggttaaa taaatctata aaagtttctc ataactggtt
     2581 agcttttaaa acaacatttt taataccatt aatagcgtca ttagcttgta tggtattgat
     2641 gatatttata ataactccat ttggtggatt aattaatgaa agtatgaaaa acttcttaac
     2701 agcagcagga gcagctggag aatatgtata tgcaacagct ttggcagcag ctacagcatt
     2761 cgacttagga ggaccaataa ataaagcagc aggattcgtt gcacttggtt taactacaga
     2821 gaatgtactt ccaataacag caagaacaat agctatagtt gttcctccaa ttggattagg
     2881 attaacaaca ttattagata aaagattagt aggaagaaga gtatatgata gacaattcta
     2941 tcaagcagga aaaacttcta tattcttatc atttatgggt atatctgaag gagcaatacc
     3001 atttgcactt gagagaccag gttttgttat acctttaaac atagttggaa gtgtaatagg
     3061 agctattaca ggtataattc taggagcaat tcaatggttc ccagagtcag ctatatgggc
     3121 atggcctcta gtagataact tatttggata tataataggt atagcagttg gtgctatatt
     3181 catagcagta ggaaatatat tctatagaaa taaattaata aaagatggta aacttgttgt
     3241 agattatatc gactagtatt taatttttac tatctataaa taactttggc tggtaatatg
     3301 gttttaatca atattaccag ccttacctat atgattaatt aaaggcggtg gagataagat
     3361 atggataaga tagaaaaagt aagagagtat ttt
//
LOCUS       DAEPOZ010000302         3376 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.303, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000302 DAEPOZ010000000
VERSION     DAEPOZ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.303"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            596..3238
                     /gene="adhE"
                     /locus_tag="KR440_003439"
                     /gene_synonym="adhC"
     CDS             596..3238
                     /gene="adhE"
                     /locus_tag="KR440_003439"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBH0700441.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPDRVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGNTDQEKVDNLIKAIDELKARVGLPKTIKEAGVEESKFLETLDVMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
BASE COUNT         1309 a          425 c          634 g         1008 t
ORIGIN      
        1 tttatactgt aaaaaatttt gtatatatat tatcattaaa aaattaacgg tcttaatata
       61 aaaattattt atctatatga aaagcataat tattacatat tgacttaaaa ataacttata
      121 gagattagaa taattttagt ttgtatattt tttaaaacca tacagttgtt ataaaaagaa
      181 aaattgcaca caagtcaaaa ataagtgtta ttttataaaa atacgacaaa aattttattt
      241 actacttata caaataacat gcaaataaca tataaaattt aataagtaaa aaaaatgaaa
      301 gatataaatt atcattttag tattttattt agttgaagta aattattcaa atgaaatata
      361 tctcatttag ataaaaaatt ttagtaaaag taaaaaaaat aatatatttg ttaaaaaata
      421 aattgacaaa atattcagaa aaaaacttct tgacctaaga gagtaaaata aataaaatct
      481 aaggtataga gatgaaatct ataaaattat tttaagtata agttaaaaat atagatggtg
      541 catttaagat ttataaaaat tagagatgca taattatttt agggaggttt ttagtatgga
      601 gaaaaaagaa aaggtagtag aaaaatcaaa tgttgaagta tgtagtcctg aaattgtcaa
      661 cagtgttgaa acattaagaa tgagattaga agaaattaga ttggcacaaa aagaatttgc
      721 aacatttacg caagaacagg tagataagat tttcttagct gcttcaactg cagctaatca
      781 acaaagaata cctctagcaa aaatggcagt ggaagagact ggtatgggta ttgttgaaga
      841 taaggttata aaaaaccatt ttgcctcaga atatatctat aatgcttata aagatactaa
      901 aacttgtggt gtcatagaaa aagatgaagc atttggtttt acaagaattg ctgagccagt
      961 aggtgtttta tcagcagtta taccaacaac taatcctaca tcaactgcaa tattcaaatc
     1021 cctaatagca ctaaaaacta gaaatggtat aattttctct cctcatccaa gagctaaaaa
     1081 ctgtactata gaagcagcta gagttgtaca tgatgcggca gttaaggcag gagcaccaaa
     1141 aggacttata ggttgggtag atgttccttc gattgaatta actaatgttg tcatggcaga
     1201 ggcagacctt atattagcta ctggtggacc tggaatggtt aaatctgcat actcttcagg
     1261 taaaccagct gttggagttg ggcctggaaa tgtacctgca ataatagatg agagtgcaga
     1321 tataaaaatg gctgtaagtt caatcttagt atcaaaatca ttcgataatg gtatgatatg
     1381 tgcttccgag caagctgtaa tagtaccaga aaaaatatat gaagaagtta aaaaagaatt
     1441 taaataccgt ggagcacatt tcctaaataa agaagaaact gaaaaagtag gaaaagtagt
     1501 aataattgat ggttcattaa atgccagaat agttggacaa ccagcacatg taatagccaa
     1561 aatggcggat gtagaagttc caaaaacagc tagaataata attggtgaag tagaatctgt
     1621 tgaattaaat gaaccttttg cacatgaaaa actttctcct gtacttgcaa tgtacaaatc
     1681 taaaagtttt gaagatgcag ttgctaaagc tgaaaaatta gttgcagatg gtggatatgg
     1741 ccatacatct tcgttatatg cagactctat aaatcatcca gatagagtag agaagtttgt
     1801 aaatgctatg aaaacttgta gagtattagt taatactcca tcgtcacaag gtggtatagg
     1861 tgacttatat aactttaaat tagcaccatc tcttactctt ggttgtggtt cttggggtgg
     1921 aaactcagta tctgaaaacg tgggagtgaa acatttactt aatatcaaga cagtagctga
     1981 aaggagagag aatatgcttt ggtttagagc accagaaaag gtttatttca aaaaaggttg
     2041 tttaggtgta gcagcaagag aatttaaaga tgttatggat aaaaagaaag cctttatagt
     2101 aacagattct ttcctttaca ataatggata cactaagaag ctaactgatt tgttggatga
     2161 gatgggaatt aagcatacta cattctttga tgttgctcca gacccaacat tagcttgtgc
     2221 aagagaagga gctaaggcta tggctgattt ccaaccagat ttaataatag ctgtaggtgg
     2281 aggtagcgca atggatgctg gtaagataat gtgggtaatg tatgagcatc cagaggtaga
     2341 cttccaagat ttggctatga gatttatgga tataagaaaa agagtatatg tatttcctaa
     2401 gatgggtgaa aaagcgtatt ttgctgctat cccaacttca gcaggaactg gttcagaagt
     2461 aactccattt gcagttataa ctgaccaaga ttcaggtgta aagtacccat tagcagatta
     2521 tgaattaatg ccaaacatgg ctataattga tgctgatatg atgatggaga tgccaccaag
     2581 attaacagca gcatcaggtg ttgatgcttt aacacatgca ttagaagcat atgtatctat
     2641 gttaagaact gaaccagctg acggtctagc actacaagca ggtaaaataa tatttgaata
     2701 tctacctcgt gcatacaaaa atggaaagaa tgataaagaa gcaagagaaa aaatggcaat
     2761 ggcatctact atggcaggaa tgtcttttgc aaatgccttc ttaggaatat gtcactcttt
     2821 agcacataaa ttaggtgcat tccaccatgt tcaacacggt gttgcaaatg cattattaat
     2881 aaatgaagta attaagttca attgtgcaga agctccaaat aagatgggtg cattctcaca
     2941 atacagatat cctgattgta tacaaagata tgctgaattt gcttcttttg caggaatcaa
     3001 gggaaataca gaccaagaaa aagtagataa ccttataaaa gcaatagatg aattaaaggc
     3061 aagagtagga cttcctaaaa ctataaaaga ggctggagta gaagaatcta aattcttaga
     3121 aacattggat gttatggttg aacaagcttt tgatgaccaa tgtacaggtg ctaatcctag
     3181 atacccattg atgagtgaat taaaagagat atatctaaaa gtttattatg gtaaataata
     3241 ttcattgtac aacataggtt ctattgggtt tgtatataaa ttattagatt tgaatataaa
     3301 tagagtttta ttagagaaac gtagaagtaa ctataaataa tagattataa cttaaattct
     3361 gaatggaaat gataaa
//
LOCUS       DAEPOZ010000303         3372 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.304, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000303 DAEPOZ010000000
VERSION     DAEPOZ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.304"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            7..999
                     /locus_tag="KR440_003440"
     CDS             7..999
                     /locus_tag="KR440_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBH0700442.1"
                     /translation="MELKDGSLVNNQYKGKIKSANLSVDYEINQVMKDIDKLGLNTVN
                     VPIIINVESINSDIMSIDNNSKEKAIKLIKKLNKKGISTILEAYPWVENGKLYETDWN
                     PINKKRFFYTWQNAILNELIIDVANPLDVNVLNIGSNFVHLEEYQQNWGEIIDFVQSK
                     FDGLVTYRTNWWYTKKDDSSSKLFYEQKLNNNFFDKLDFISIAAYFELSNKPVNTVDE
                     LISALHSSTVNNRGQNIKQEIYNLYKAHRKPIFFGELGFSNRESASSQPWNHTPSKAV
                     NGEEQARCFEAYKKVFENEDWINGFSVFCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            1053..2177
                     /gene="wecB"
                     /locus_tag="KR440_003441"
     CDS             1053..2177
                     /gene="wecB"
                     /locus_tag="KR440_003441"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBH0700443.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKALVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDSYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            2255..3229
                     /locus_tag="KR440_003442"
     CDS             2255..3229
                     /locus_tag="KR440_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBH0700444.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNDKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSVTIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQALENAGYSTAEDKKGNRIYAELLIDIIRSYNLQLIDNKVETK"
BASE COUNT         1409 a          332 c          599 g         1032 t
ORIGIN      
        1 gtaagtattg agttaaaaga tgggagtcta gtaaataatc aatataaagg taagattaaa
       61 tcggccaatt tatctgtaga ttatgaaata aatcaagtta tgaaggatat agataaattg
      121 ggattgaata cagtaaatgt acctataatt ataaatgtag agtccattaa ttctgatatt
      181 atgtcaatag ataataatag taaagaaaag gcaattaagt taataaaaaa attaaataaa
      241 aaaggtattt caacaatatt agaggcatat ccatgggttg aaaatggaaa attgtatgag
      301 actgactgga accctataaa taaaaaaaga tttttttata cttggcaaaa tgccatatta
      361 aatgaactta taattgatgt agcaaatcca ctggatgtaa atgttttaaa tattggttct
      421 aattttgtac atttggaaga atatcaacaa aattggggtg aaataataga ttttgtacaa
      481 tccaaatttg atggtcttgt aacgtacaga actaattggt ggtatacaaa aaaagatgat
      541 tcaagtagta agttgtttta tgaacaaaaa ttgaacaata atttttttga taagttagat
      601 tttatatcta tagcagcata ttttgagtta agtaataaac ctgttaatac agtggatgaa
      661 ttaatttctg ccttacactc atctactgta aacaatagag gacaaaatat aaaacaagaa
      721 atatataacc tttataaagc tcatagaaaa cctatatttt ttggagaatt aggtttttca
      781 aatagagaaa gtgcaagttc tcagccgtgg aatcatactc cttcaaaagc tgtaaatggc
      841 gaagaacaag caaggtgttt tgaggcatat aagaaagttt ttgaaaatga agactggata
      901 aatggttttt ctgtgttttg tgtaggtaaa atagatgacg aaaaaaattt ttacccatca
      961 aaagagagta taaaagttat taagtcttgg tatgaataat tttaaaagtg tagaatttat
     1021 atagattaaa tagatgatta ggagagatat aaatgaatga catcaaggtt atgactgtat
     1081 ttggaactag acctgaagct atcaaagtag cacctttaat aaaggaactt gaaaaaagag
     1141 agaatataaa aagtattgtt tgcgttacag ctcaacatag agaaatgctt gaccaagtta
     1201 tagaaacctt caatataaat gtagactatg atttggatat aatggaaaaa ggacaaagcc
     1261 tgaatgatat aacatgtaag attttaaata aacttccctt gattttaaac aaggaaaatc
     1321 caaatataat attggtacat ggagatacaa ccacaactct agctacaagt ttgactgcat
     1381 tttataataa agctcttgtt ggtcatatag aagctgggct tagaacctat gataaatatt
     1441 ctccttttcc agaggagtta aatagacaac ttacaggtat tattgctgat atgcactttg
     1501 ctcctactaa tttggctaga aaaaacttga tttctgaagg aaaacctaat aataatatat
     1561 ttgtaactgg taatactgct attgatgctt taaagatgac aataaaagaa aactataatc
     1621 atcctataat tgatgagatt ggtaacgata ggatgatttt gttaacctct cacagaagag
     1681 aaaatttggg taaacctatg aaaaatatat tcagagctat aaagcgaata gtagatgatt
     1741 ttgaagatgt acagatagta tatcccatac atttaaatcc taaaatacga actatagctg
     1801 atgagatatt tggaaaattt cctgaaaaaa ttcatattat agagccttta gatgtagctg
     1861 attttcataa ctttttaaat aaatcttata tgataatgac tgatagtgga ggaatacagg
     1921 aagaagctcc atcattaggt aagccagtcc ttgtgttgcg tgataaaaca gaaagaacag
     1981 aaggaattga agcaaagaca cttaaattag taggtactaa tgaagataga atatataatt
     2041 cagtgagcga tttattaata aataaagata gctatgttca gatgagtaaa gcatctaatc
     2101 catatggaga tggaaatgca agcaaatata tagtggatat aataattaaa aaatttaatt
     2161 gcaaatattt aaattagata aggtctaaaa cattaactaa ttgatataag atttatgata
     2221 atattaagga atacaatttt taagggggag taatatggct agaaaattaa taaaaaattt
     2281 aggtaaaagt aaaagtgtaa agagagtgaa acttttattt aagaagattt ttattactgt
     2341 attcatagta gcaagtatag ttgctatttt taatataacg aaatattttg aggaattata
     2401 caaagtaagg gatttaaaaa gtactaaaat tgaatactat atggatgtgg ctgatgaagc
     2461 aggagatgga aaggtccaat taagttggaa ggccttactt gctatagaca tggtaataca
     2521 tgatgaagat ttaagtaata taaaaaagaa agacacattg gatatagggg aaaagtttat
     2581 agtagaagat aaaaatgata aaggcgaaaa ggtgtataag gtaaaaaagt ttaataaggt
     2641 attaagcgaa ttgaaatttg actcttctca aaaaagtaga gcaagaaaat acatgaaaga
     2701 tttggaatac acataccttg gaaataaaca attagatagt agtgatgaaa aaattaaatt
     2761 tataaagaag ttagaagact cagctataag agaatatatt gattatggaa tattgccctc
     2821 tgtaacaatt ggacaagcta tattagaatc tggctgggga aattctaaac ttacaaaaca
     2881 gagtaataat ttatttggta taaaagcaga taaagcatgg aaaggaaaaa gtgtagaaat
     2941 ttcaacttca gagcattata atgaaaaaat tgtagctagt tttaggtcgt ataattcatt
     3001 acaagattct gtcaaagacc acagtttatt tttaattaat aataaaagat atagaaaaca
     3061 tgggttgttt gaggcaaaag attatattag tcaagctcaa gcattagaaa atgcaggata
     3121 tagtacagca gaagataaaa aaggaaatcg tatatatgca gagctattaa tagatattat
     3181 taggagttat aatttgcaat tgatagacaa taaagttgag acaaaataat tgttaacttt
     3241 atatgaggag ataacaagat aagattcttt tctacaaaaa gaatagaaaa attgtaaaaa
     3301 aaactaaaaa aatgtattga cttgttattt aaaaggtgat attattatta ctgtactcga
     3361 aagagaacaa aa
//
LOCUS       DAEPOZ010000304         3343 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.305, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000304 DAEPOZ010000000
VERSION     DAEPOZ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.305"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..201)
                     /locus_tag="KR440_003443"
     CDS             complement(<1..201)
                     /locus_tag="KR440_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700445.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTP"
     gene            complement(225..1019)
                     /locus_tag="KR440_003444"
     CDS             complement(225..1019)
                     /locus_tag="KR440_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HBH0700446.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            complement(1585..2061)
                     /locus_tag="KR440_003445"
     CDS             complement(1585..2061)
                     /locus_tag="KR440_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBH0700447.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPNVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSVPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            complement(2162..3331)
                     /locus_tag="KR440_003446"
     CDS             complement(2162..3331)
                     /locus_tag="KR440_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0700448.1"
                     /translation="MLNIRTLCHEINDWIINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKNYLNVNVKFFFQPAEETIGGAQLMIEDGCLENPNVKYI
                     FGLHVNPNINKNLIELKYNTLNASTDTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF"
BASE COUNT         1176 a          505 c          451 g         1211 t
ORIGIN      
        1 tggagtggtt atacaaaaat atattaaaaa gaaaattaat acagctccaa gagatttata
       61 ttcaaatcct aagaatgata agaagccaat tcctataacc gtgaaaatac aaaaagttat
      121 accaaaaaaa acagcaaaaa ataaaaatac agaaataaat tcgcctaaag gaatccttct
      181 tttattactt atcttaatca actccttaat ttatgatatg aaatttatat aaataataac
      241 attcttaaaa attcataata gtcctctgtt tcaaatagta ctgtttgaga atctattttt
      301 ttcatatttg gataaaaaga ggccttaaaa gctttataat gttctctaaa ttttatttct
      361 atcttaaatt tatctggcaa ttttattaag tgcctagata aatctccaga taaagctttt
      421 ttgacacctt tttcaatttt ttttagtgca aggtttggat ggattgaaat tgaaccattt
      481 ccaattcctt ttgacacagg aacagtgact atatttggat ttagtttttt tgcactctca
      541 caaagcattt catctccact taaaaatgct acaggaacat tataatatgc agcaatataa
      601 gcatttatag taaattcaga tgctatttca tcatttattt taaaatagtc ataatcaata
      661 tccatagtat gagaaagtgg attaccatta caagtagctc cagagtgata gccggtgaat
      721 atacatgcat caaagctttc atcaattcca gccatcataa ctagtgggtc tttggtccat
      781 cctctaagta ttttgacgtt ttcaggtaaa atatttggat taatatttct accactgtca
      841 tgtgcatctt taacaagtat ttcatgtaca ccagattgag tagctccatc acatgctgct
      901 gttatctctt tactcatttg tgttctatag tgttgtgaaa attcacattc aagttttgtt
      961 tcatcccaat ttacaatacc acaagtacct tctatatcag cacttaaata caatttcata
     1021 atatataccc ccttatgttt attattgatg ataataacag ataacagaaa atacataatc
     1081 aataaagggc ataaaaatat tatgtcttac tattaattat tattaaatct gtaactgaaa
     1141 aataactaaa ttggtattaa aaaatataac actatgtgtc atttatttat atagtttttt
     1201 atatctgatg tttatataaa attgtaacat ataagtggtt acatttacta gtttaacata
     1261 gttaaaatag ttaggtatta tttttaagtt aaattgatta ttataaaatc gtagaataaa
     1321 agaaaaattt aatttattaa aatgcatgaa atgttttttc ctcaacataa tgctaaatta
     1381 aactcaaaaa gaaataatag aaaaatctta tgtagcttag aaagaatgtg aatgatttaa
     1441 gggaaaaata gaaatttatt atataatatt aggctgtatt atgtaataca gcctaatatt
     1501 atatgtatat gtgacataaa atcttggtaa gtgtaaataa aataaatgtg gaaaacaaat
     1561 taatgaaatc aagaaaaaag taatttaaaa ttcaatttca tcttcccaat taaattctcc
     1621 tgatttttct acactcttag gccagtgact acaccattta ggaacggacg gattttcaac
     1681 cctatcaacg cttggagtat atggctttaa gtctataaca ggactattat cattagcatc
     1741 aatatatggt atttttatta taccattttc atggtcgata ttgataattt gtacagcagt
     1801 caatgctatt ggattgggtc ttataggtga ccttgttgca aatattccta taacgtttgg
     1861 tccactttta taaggttttg gggtttctaa aatacttctt agttcaggat tatcaaaccc
     1921 atcagcccac caaaatacat ttaaatgact gaaatcgtcg agttcattta atgcaggtac
     1981 atactttttg tctaatttaa taaagacctc ttcgccttga atacatattt tacctatagg
     2041 atttattaat atttctttca taatattacc tccaactatt taatttatca tttgtaattt
     2101 tagggtataa catgacatca tgtgagggtc aatataaaat aataaataaa aatattaata
     2161 gttagaatga taatacattt tttacatgga gcatgacacc agtaataagg caatcttcgt
     2221 caatatcaaa agtagaggta tgcaaagggg aagtaatgtt tttttcacga tttgcacaac
     2281 caaggtggta aaaagctcct tcacagtgtc ttagaaaata cgaaaagtct tcagccccaa
     2341 gagtgggcga gtctttaagg ataatatttt cttctccaaa caattctaca gcattttgct
     2401 tgacgtaatc tactagatgg tttgaattga taagagctgg ataaccttcc tcaatttcaa
     2461 cagaaatact tcctccaaat gttttacaag taaaatcgca aatttctcta attcgttttt
     2521 ttgaaaactc tctagtttca ggagtcaagg ttctcaaagt accccttata accactttgt
     2581 cacaaactat attttccttg attccacctt caatctttcc tagagaaata accacagaat
     2641 cagttggatt tgtgtttcga ctaacgattg tctgtagagc tgtaataata tgagcagata
     2701 taacaatagc atcaatacct tgatgtggat aagctccatg acattttgaa ccatgtacag
     2761 ttaattgaag tgtatcggtg gaagcattta gagtgttgta ttttaattca attagatttt
     2821 tgttgatatt gggatttaca tggagtccaa atatatattt tacatttggg ttttcaagac
     2881 atccatcttc tatcataagt tgagccccac caatagtttc ctcggcaggt tgaaaaaaga
     2941 attttacatt tacatttaag taattcttta atttaaatag tatattacaa gtacccagta
     3001 aaatagcagt gtgtgcatca tgaccacatg catgcatttt accaatatta atagatttat
     3061 aagaataatt aagttcttct gttataggta aggcatctat atcagctctt atagcaactg
     3121 tttcaaagtt tggactcacg ttaatgtatg cagttatccc tgtatgattc ttatattcaa
     3181 tataatttat acctatttca tttaagtatt taataatttt tttcttagtt tgaaattctt
     3241 ctaatccaag ttctggagtc ttatgtaaat ctcttcttat atttataatc caatcattta
     3301 tctcatgaca aagagttctt atattaagca taaaacacat cct
//
LOCUS       DAEPOZ010000305         3287 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.306, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000305 DAEPOZ010000000
VERSION     DAEPOZ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.306"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..909)
                     /locus_tag="KR440_003447"
     CDS             complement(<1..909)
                     /locus_tag="KR440_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0700449.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIIFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLTLANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGSK
                     FVGGKSIVFSIIGIIFLIILISGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     ADIYNSLNILNEEIHRSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPEKVYKYASIMLKNIEYTNTLIND"
     gene            1408..2268
                     /locus_tag="KR440_003448"
     CDS             1408..2268
                     /locus_tag="KR440_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBH0700450.1"
                     /translation="MKKQKSPILLEMLGLFLGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
     gene            complement(2508..3065)
                     /locus_tag="KR440_003449"
     CDS             complement(2508..3065)
                     /locus_tag="KR440_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0700451.1"
                     /translation="MSDSEYPQMKTLETRSCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
BASE COUNT         1192 a          427 c          397 g         1271 t
ORIGIN      
        1 atcgtttata agtgtatttg tatattctat attctttaac atgattgaag catatttata
       61 tactttttca ggtgaataat tagtactact atctgtaaga agctctgcat acccttttat
      121 tggtgaaagt ggagttttta aatcatgagt tatatttgaa atccactctt ctctcataat
      181 atcagtttta ttttgtatct catcacttct atgaatttct tcatttaaaa tatttaaact
      241 attatatata tcagcaaaaa cacctttaat attaactgga agatagcttc gttttgaaat
      301 atcactaaca gcagatgtaa tatttgatat gcactttgtt acccagtaac cataagcaat
      361 tccagaaatc aaaattatta aaaatataat ccctatgatt gaaaatacaa ttgacttacc
      421 acctacaaat ttacttccat caaaaaacat attcacttta gatatattta atggaaaata
      481 tataatataa atatagtcat ttttattatt tttaattgta ttaaagtata cagttccatt
      541 acttgaatta ttttggttag ctaatgttaa taattcagaa ttagaataat aatctttatg
      601 ctcttttggt tctaaaaacc cataaactct atttccacta gaatctaaaa tttgcaacca
      661 caattgattt tctcgaagct tttctaatcc agactgtttt acttgtggct tattatctat
      721 aaatatgatt tgttcattaa agttttctgt aaattttttc ggccagtcac tacgaataaa
      781 ttccccattt ggtttctgaa tagtaataag tgataagaaa atacctatta ctgtcaggat
      841 taagcaaata aaaatcatga aaaaaataat gtatgaatga aatactgttt tataactagg
      901 tttatgcatt taatcaactc ttttcttaaa cttttatttt agtttgattg ttgcttatat
      961 aaaatagata ataattattg atatgtaaaa atttatttaa aaatacaact atctacataa
     1021 attatattta tataattata tgttatcaag ctgtctgttg tatttaataa atccttttga
     1081 taacatataa aagcaagata attttttcca ttaaatacct tataattata cacaaaatct
     1141 ctatatacgt taaacagtat agagaaaatt ttcatagaac catctttata ggggatactt
     1201 tataaaagaa taaaaaaatt ataatatata aaaaacttca taggagtgta acaacatcag
     1261 taaagatatg aaaacactat ataaaaattc aaaaatatta agaataatga aatacttgaa
     1321 attgcttata tcaagaaatt ttatataata taaactggtc aaatatgtta taatattaaa
     1381 ttgtgacaag ttcatggagg tgtttatatg aaaaaacaaa agtcgccaat tttacttgaa
     1441 atgcttggac tatttttggg ttgtatatca atgagtattg gtataaatat gtttttaaaa
     1501 ccacatacaa ttgctcctgg aggacttagt ggtctatcat tagtactaaa caaggttaca
     1561 ggacttccag tatcagctat catgttaatt ataggtgtcc cattagtaat acttgctttt
     1621 agaattatgg gtacaaaaaa ttcattaaaa actttatttg gaacagttgt attttcagct
     1681 atagttcaat taacagaccc attatcaaaa ctaagattta ctaatgactt attattgtct
     1741 tctattgcag gaggaatcct agttggtata ggtttaggaa taatgtttaa atcagatgct
     1801 tctactggag gaacagactt aattgcactc atacttagta agaaattccc aggaataaaa
     1861 gctacaaaat tcatgtcatg cctagatgga atggttgtta tatcttctgg tatagtaaat
     1921 cgtagtttag aaactgctct ttactctggt atagcacttt gtgtattaat aaaaatagca
     1981 gatatgataa tggaaggatt tgaccattca aaagcatttt ttattatctc tgatgagcct
     2041 gaaagtttaa gacaagctat aactgaagaa cttgatagag gtcttactat tcttgatggt
     2101 aaaggtggat acactagaga aaataaagaa gttctattag tcgtagtttc taaaaaacaa
     2161 gaactttatc taaagagatt agttaaaaga actgacccta ctgcatttgt tattgttaca
     2221 gatgtgcatg aggttcttgg agaaggattt aaaaatttag agaactaata aaaattaata
     2281 ttttaagtat atatactatt ttaattaaaa tacgatttat tcaaaaagag tgtctcataa
     2341 actttttagt tcatgagaca ctcttttttg tataaaatca aatgcatttt cagtatttta
     2401 acaataaaaa acaggattat ctatattttg agtcaactac tttattgaaa ttttatttag
     2461 aaaacaatca ttactaaata accaggatag atattaaatg attatatcta ttgttttttc
     2521 aattcatatt gataacaagg aacatttttt acagtatttc cagtatgaat ttttttgata
     2581 tactttcctg tacatttaaa tcctaataaa tctaatgatt ttgtagaatt ttggttttct
     2641 tcataggtta ttgcaattat tttatttaag tttagttctc taaatccata ctgtattact
     2701 ctttttgtta tctcagtagc aaaatcatgt ccccaatagc ttggatttat acatatacct
     2761 atttctgctt ctaatgcttt aggatttata ttattaaacc ccatattccc aataacttta
     2821 ttgtctcttt tatatactat agcaaagtgg ccaattttcc cttttttata gggatttaga
     2881 aaaaatgatt ttataaactt tcttgtatct tctaaagatt tatgctcact aaatggtaaa
     2941 tgttttacta ctattttatt tttataacag tcaaaaaaat cttcaacatc atctaatgat
     3001 gcaggtctta gtatacaact tctagtttct aaggttttca tttgaggata ttcagaatca
     3061 gacatttcta atcccccttt atacattaat ctaaatatac tgttcatatt taaaatacga
     3121 tattaattat ataaatgttc tcaaattaac cttatcttta tttttaataa aaactaatta
     3181 ctatatgaat ctttcaaatt tacgaattta tctctatttt tatattaaat tacttgtgaa
     3241 atatgtattc taaattatat gttattagat atcatgctta aaaattc
//
LOCUS       DAEPOZ010000306         3286 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.307, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000306 DAEPOZ010000000
VERSION     DAEPOZ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.307"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            196..1113
                     /gene="pfkB"
                     /locus_tag="KR440_003450"
     CDS             196..1113
                     /gene="pfkB"
                     /locus_tag="KR440_003450"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBH0700452.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     ILKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYAKRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKQF"
     gene            1150..3069
                     /locus_tag="KR440_003451"
     CDS             1150..3069
                     /locus_tag="KR440_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0700453.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVAEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
BASE COUNT         1259 a          354 c          649 g         1024 t
ORIGIN      
        1 ttgaaaaatt tgtaaattta tttaattaga tgggtagtat tgtatataca cctgtaaatt
       61 tggataatat aaaactaaat aagataaaaa cgataaattt ctcttttaat taagagaaat
      121 tttttgactt tcaaggatat taatataaag ttaagatata aacataaagc aaataaataa
      181 aaaagaggtg gcaatatgat ttatacagtg acattaaatc catcaataga ttatatagtt
      241 aagctagatg agctaaagac aggaagtaca aatcgtgtaa aagaagaata cgtttatcca
      301 ggaggaaaag gtataaatgt atctcgcata cttaaggaac ttggaaatga taatacatct
      361 ttaggtttta taagtggttt tactggagaa tatataataa gaacattgga agaaaaagaa
      421 ttgaagacag attttataaa gataaaaagt ggtttttcaa gaataaatgt aaaaataaag
      481 gcgttaaagg aaactgagat taatggacaa ggtccaaata tagatgatga agacattgat
      541 actttatata aaaaacttga taagctaaat caagatgata ttttaatatt ggcaggaagt
      601 ataccatcta ctttagatga aaaactatac gaaaatataa tggctagatt agaaaaaaag
      661 aatataaaag ttgtagtaga tgcaactaaa aatcttcttc ttaatgtttt aaaatacaaa
      721 ccttttctaa ttaagcctaa taacgacgaa ttagaagaac tatttggtgt taaattaaat
      781 tcaattgaag acatggtaaa atatgctaaa agattaaagg aaatgggagc tataaatgtc
      841 ttggtatcaa tgggaaaaga tggagcattg ttgattacag aggaagatga agtgcttata
      901 agtgatgtcc ctaaaggaaa agtaaaaaat tctgtaggag caggtgattc aatggtagca
      961 ggatttatat ctgggtattt gaatactggg aaatatgatt atgctcttaa attgggagca
     1021 gcttcaggta gtgcaactgc tttttcatat gacttagcca aaagagaata cattgataaa
     1081 ttggtaaatg agatatctgt aaagcagttt taaaatatta aaaaatatat agaaaggtaa
     1141 ttaatattta tgaaaattat agatttatta gatgaaaagt caattaagtt aaatttaaat
     1201 tcaaaaacca aatcagaagc aatagaagaa ttagtagatt tggtcgcaaa tagtggaaat
     1261 ttaaatgata aagaaaacta caaaaaagca attttggctc gtgaagaaat gagtacaaca
     1321 ggaattggag aaggtgtagc tatacctcat gctaaaaatt cttcagttac taaagcttgt
     1381 atagcagcag cagtgtcaaa agaaggtata gattatgaat cgtttgacgg aagtttatca
     1441 aatctatttt ttatgatagc tgcaccagac ggagctaaca atactcatct tgaagtatta
     1501 tcaagattat ctacaatact tatggatgaa gactttagaa ataaacttat aaattcaagc
     1561 tctgaaaaag aatttaagga aataatagat aaaaaggaaa gagaaaaatt cagtgaagaa
     1621 tatcaagatg agaaagtggc tgaaaaaaat ataatagata ctaaagagaa tgatgctaat
     1681 aagtatccta aggttttagc agtaacagca tgtccaactg gaatagcaca tacatttatg
     1741 gcagctgaaa gtttaaataa aatggcagaa aacaaaggtg tatcaataaa agtagaaact
     1801 aatggttcag caggtgttaa aaataagctt acaaaagatg aaatagaaaa tgcaacatgt
     1861 ataatagtgg cagcagataa aaatgttgag atggctagat ttaatggcaa aaaagtcata
     1921 aaaactaagg tggctgatgg tatacacaaa gcggaagaac ttatagacaa ggctgtaaat
     1981 ggagatgctc caatttacca tggaggagat ggaagtcaca acgaatcaaa tgaagaaagt
     2041 gaaagtggat ttagaaaagt atacaaacac ttaatgaatg gtgtatctaa tatgttacca
     2101 tttgttattg gtggagggat acttatagcg atagcctttt tacttgatga ttatactatt
     2161 aacccttcaa actttggttc aaatacaccg atagcagcgt tttttaaagg gataggagat
     2221 aaagcttttg gatttatgtt acctgtactt gctggatata tagcatactc tatatctgat
     2281 agaccagcgt ttgttgtagg atttgtagga ggtgctctag caggtgatgg tggttcagga
     2341 tttttaggtg cactacttgc aggttttata gcaggttatt tagtagaagg gcttaagaaa
     2401 atatttagtg tactaccagc ttcattggaa ggaataaaac cagtattgtt atacccttta
     2461 cttggaacac ttttgatggg aattataatg acatttttag taatacctcc agttacagca
     2521 attaataatg caatggttgg atttttaaat ggtttaggcg gaacttcaaa aatagtttta
     2581 ggattagtat taggtggaat gatggctgtg gatatgggtg gacctgtaaa taaagcagca
     2641 tatgtatttg gagttgcatc acttgaatca gggcagtttg aaataatggc agcagtaatg
     2701 gctggtggta tggttccgcc acttgctata gctcttgcaa ctacattttt taagaataga
     2761 tttactaaag aggaaagaga ttctggtaaa gttaattatg taatgggatt atcatttgtt
     2821 actgaaggag ctataccttt tgcagctgga gacccacttc atgttatacc agcttgtgtt
     2881 ggaggttcag cagtggcagg agcacttagt atgttgttca acgcagctct tcgtgcacct
     2941 catggaggag tatttgttat accagtggtt acccatccat ttgcatatat attagctata
     3001 gctgtaggtg ctttggttgg aatgatgctt ttagcattac ttaaaaaacc attaaatcaa
     3061 gaagtatagc aattaaagga tattcaagaa cttaaaatat gatgttttat aaaaattaat
     3121 atattgctga attattttaa aataaggatt ttattaaaat aaggatttta ttaaaataag
     3181 gattttatga ggctgttata taacgatttg taattgttat cttgacagtc ttttattttt
     3241 atataagtaa aataagtgtg ttttgcttta tttgtattta aattgt
//
LOCUS       DAEPOZ010000307         3285 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.308, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000307 DAEPOZ010000000
VERSION     DAEPOZ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.308"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            476..1885
                     /locus_tag="KR440_003452"
     CDS             476..1885
                     /locus_tag="KR440_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HBH0700454.1"
                     /translation="MIFSSLIFLFLFFPVTLICYYLANPKYKNVMLLLASIAFYAWGE
                     PKYIFLMILSIIVNYIFGIKVSGSKNKKMWVVLSVIYNISILAVFKYSNFFIDNINFL
                     FNTDITIPEVKLPLGISFFTFQIMSYVIDVYRNDAKVQRKLANLALYISLFPQLVAGP
                     IVRYQTVADSIEYREHNWDKFTQGVNRFVVGLGKKVILANQLGVIADGVFNKSAGDLS
                     RLEVWLGIVCYTLQIFYDFSGYSDMAIGLGKMFGFEFLENFNYPYISQTVSEFWRRWH
                     ISLGTWFKDYVYFPLGGSRVSKAKLYRNLFVVWFLTGMWHGASWTFIIWGLYYGILIS
                     VEKAFLEKFLYKMPRIVRHIYLLLIVMIGWVFFRVDDLPAAIKFIGIMFGAGNYPVIS
                     DAFNVFIIEYWYIIVGAILLSTPIVKVVKSLLIKKNKNTLNSTLYQVTNVVFIVSCMF
                     IVIIMLSSSTYNPFLYFRF"
     gene            1901..3103
                     /locus_tag="KR440_003453"
     CDS             1901..3103
                     /locus_tag="KR440_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700455.1"
                     /translation="MSKKKPRIVAISFLIIVFGFFILNFTLPKKKLSITENRNLAKFP
                     TVENLKKCDFIAKFEEYFNDHFAFRDEMISINTRTQANLNKTKVGNYYLGKDNWILGM
                     FPEILDQKQLSRYEKSINYLSNLSKDMGKNVYFTMMPHKTNMLKHLYPKYVDNMDNID
                     INLKNFKNRLNPDLIKYIDMDEYFLSNYSDKERENLYFKTDHHWTGYGAFEGFKMMAS
                     NMDLGLSKQDLDKHFSSYKKSIITDKKFIGSYNQNIDMLIKENEYVVYMTKDNQDYSF
                     DINGEEKEEKEEKEVYATERDNKEWDYGGAYIRGAKTNILTIKNPKALVDKKILIFRD
                     SYQAPTTLLFADMFKEVEIADPRNINSIYKTYKEIIEESNADIVMFMYNSSGFDSMID
                     SMIDKGIK"
BASE COUNT         1275 a          347 c          506 g         1157 t
ORIGIN      
        1 acaacaaatg gaaactatgg aagcaataat taaaataaaa tttataatca atcataatta
       61 tttagtataa tgattcgata ttattttaaa gtatttgtca tttaaaacaa acttgcgata
      121 aaagcatatc tgaaagtatt ctgattctaa tacttaataa agagttcttt ttacagttat
      181 agatataaac aacatctatc ttaatagact gtatagaggc acaatcaaag gtaaacacat
      241 atagttaagt aatatgtcct tgatttttct ttattataat agctttatta attcatttta
      301 atattacatc attttaaaaa aattatatga aaagtcaaaa tttacatata gattaattaa
      361 acttgtaaaa acacatataa attcatacaa ttaagtactt aaaacttgaa aatacttact
      421 ttgctatata ttttagataa agatataatt gatttggtta agaaaggagt ctaatatgat
      481 attcagtagt ttaatatttt tatttttatt ctttccagtc acactcattt gctattactt
      541 ggcaaatcct aagtataaaa atgttatgtt acttctagca agtattgcat tctacgcatg
      601 gggagaacca aaatatattt ttttgatgat actatcaata atagtaaact atatatttgg
      661 tattaaagtt tctggcagta aaaataaaaa aatgtgggtt gtgctttctg ttatttacaa
      721 tatatctatt cttgctgtat ttaagtatag caattttttt atagataata ttaatttttt
      781 attcaataca gatataacta tacctgaggt taaattacct ttaggtatct catttttcac
      841 attccaaata atgagctatg taatagatgt atatagaaat gatgctaaag ttcaaagaaa
      901 attagctaat ttagcattat acataagttt attccctcag cttgtagcag gtcctattgt
      961 tagatatcag actgttgcag atagcattga gtatagagaa cacaattggg acaaatttac
     1021 tcaaggtgta aacagatttg ttgttggtct tggaaaaaaa gtaattctag caaatcaatt
     1081 aggtgtaatc gcagatggtg tatttaataa gtcagctgga gatttatcaa gacttgaggt
     1141 atggctaggt atagtttgct atacacttca aatattctat gactttagtg gctatagtga
     1201 tatggccatt ggtttaggta agatgtttgg atttgagttt ttagaaaatt tcaactatcc
     1261 atatatctct caaacagtat ctgagttttg gagaagatgg catatatctt taggtacttg
     1321 gtttaaagac tatgtgtact tcccattagg tggaagtaga gtctctaagg caaaactata
     1381 tagaaatcta tttgtagtat ggtttttaac cgggatgtgg catggagcta gttggacttt
     1441 tattatatgg ggtctatatt atggaatatt aatatcagta gaaaaagcat tcttagaaaa
     1501 atttctatat aagatgccaa gaattgtaag acacatatat ctacttctta tagtcatgat
     1561 tggatgggta ttttttagag ttgatgattt accagcagct attaaattta taggtattat
     1621 gtttggtgct ggaaattatc cggttatcag tgatgcattc aatgtattta taattgaata
     1681 ttggtatata attgttgggg caatccttct atcaactcct attgtaaaag ttgtaaaatc
     1741 attattaatt aagaaaaata aaaatacatt aaatagcaca ttatatcaag ttactaatgt
     1801 ggttttcatt gtttcttgta tgtttattgt aataataatg ttaagtagct caacctataa
     1861 tccattctta tattttagat tctaattggg aggtataaac atgagtaaaa aaaaaccaag
     1921 gattgttgcc atttcatttt taattatagt atttggattt ttcatactaa acttcacact
     1981 tccaaagaaa aaattaagta taactgaaaa tagaaatctt gcaaaatttc cgactgtaga
     2041 aaatttaaaa aaatgtgact ttatagccaa gtttgaagaa tattttaatg accattttgc
     2101 atttagagat gaaatgatat caataaatac aaggactcag gctaatttaa ataaaactaa
     2161 ggtaggtaac tattatctag gaaaagataa ctggatatta ggtatgtttc cagaaatttt
     2221 agaccaaaaa caattaagtc gatacgaaaa atccattaat tatttatcta atctaagtaa
     2281 agatatggga aaaaatgtgt attttacaat gatgccacat aaaactaata tgttaaaaca
     2341 tttgtatcct aaatatgttg ataatatgga taatatagac ataaatttaa agaattttaa
     2401 aaatagatta aatccagatt taattaaata tatagatatg gatgagtatt ttttaagtaa
     2461 ctattcagat aaagaaagag aaaatttata ttttaaaact gaccatcatt ggactggata
     2521 tggagcattt gaaggcttta aaatgatggc atcaaatatg gacttaggtc tatctaagca
     2581 agatttagac aagcatttta gtagctataa aaagtctata attactgata agaaatttat
     2641 aggaagttat aatcaaaata tagatatgct aattaaagaa aatgaatatg tagtttatat
     2701 gacaaaagat aaccaagatt atagctttga tataaatgga gaagaaaaag aagaaaaaga
     2761 agaaaaagaa gtttatgcaa ctgaaagaga taataaagag tgggattatg gtggtgcata
     2821 cattagagga gctaaaacta atatattaac tataaaaaac cctaaagcat tagtagataa
     2881 aaaaatacta atatttagag attcatatca ggctcctaca acacttttat ttgctgatat
     2941 gtttaaagaa gtagagatag cagaccctag aaatataaac tcaatatata aaacatacaa
     3001 agagataatc gaggaaagta atgcagatat tgtaatgttt atgtataata gttctgggtt
     3061 tgattcaatg atagattcaa tgatagataa aggaataaag tagtaaataa aaagcctcta
     3121 ttaaattcaa aatttaatag aggcttttta ttgtttattt tttattgctt tggtaaaaat
     3181 aaactaatca aaataaaagt aaggagatgt agcatattga acaaaagaca tagtctacct
     3241 atagtatcag tgatattatt gatggtctta attatcttta ataat
//
LOCUS       DAEPOZ010000308         3269 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.309, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000308 DAEPOZ010000000
VERSION     DAEPOZ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.309"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(145..666)
                     /locus_tag="KR440_003454"
     CDS             complement(145..666)
                     /locus_tag="KR440_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBH0700456.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENIIIKTEVPETAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
     gene            794..2158
                     /locus_tag="KR440_003455"
     CDS             794..2158
                     /locus_tag="KR440_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBH0700457.1"
                     /translation="MKESNKKIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYIARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDKLHIAIKTSILWTTIFCVVLGL
                     ILVLFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            2347..3024
                     /locus_tag="KR440_003456"
     CDS             2347..3024
                     /locus_tag="KR440_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBH0700458.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSDYKKGNPVH
                     CGDSDCIGILIIKSGTIRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQINSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
BASE COUNT         1030 a          455 c          506 g         1278 t
ORIGIN      
        1 aaaagtttaa cattctccct caattaagtg agctttattt taaatatact tttcagttta
       61 tatcatttgt ttcatttttt ctaatatctc attattaaat atgctggttt ttgtttaatg
      121 attattaagt tagcttctct aattctaatt ccccatatta tcttttaaag aatttatcca
      181 ggtttccaat gcttgtgcta gcaccaactg ttgctccaac actgcttttg ctgtctctgg
      241 tacttctgtc tttataatta tattttcctc aatagttttc tttaactctt gaacattctc
      301 ttcaatatta ttgagacata atttctgttt ttctggtgta aggctattta aatttactat
      361 aacagcatta aaatctaaaa gaatatttat tggtttgcta gatgtatcaa acattaaatc
      421 taaaaaatgt gtctcaccta attcagttaa tgaataaact gctttttctg gcatttttcc
      481 attcttcaca gttttacttt caatatatcc cttttgttct aattgaatta cttttttata
      541 aattgatggt gtacttattt taacccaacg tgatatattc ctgtattcca ctgccttttg
      601 aatatcataa gcactcttgg gttgttgctt tactattccc aatacaatta aatctattgt
      661 agccataaaa atcatctcct ttagttttct tactattgac aaatactata aattatagta
      721 ctattttcca tacttactat aatttatagt attataattt agacttaatg tcaatagaag
      781 aggagtgtta agtatgaaag aaagtaacaa aaaaattgaa ttattagggt ctgccccaat
      841 ctcaaaagca ttgttatcta tgggattacc taccatgata ggtatgatga taactgcatt
      901 atacaatttg gttgatgcat actttgtggg tggacttgga acaagtcaaa tgggagctat
      961 atccgttgca tttccattag gtcagcttgt tgtgggatta ggattattgt ttggaaatgg
     1021 agctgcttca tatattgcca gacttttagg ccatggtgat aatgaaaaag caggaaaagt
     1081 ggtaagtaca tctatttaca gtagtattat agtgggtaca ttagttatta ttgtgattat
     1141 gatttttctc aatccaattt taaaattgtt aggagctaca aaaagtatta tgccttatgc
     1201 aatatcttat gctagcattt atgtcgtgtc atctattttt aatatattca atgtcactat
     1261 gactaatatt gtcacaagtg aaggagctgc taaaacaact atgtgtgcta tgttattagg
     1321 tgctttgctt aatatgattt tagacccgat attcatttat acttttggtc tgggggtaag
     1381 gggtgcttct attgcaacag ctatctcaca gattgcttca acacttgttt atgtaggata
     1441 tattttaagc aagaaaagta tatttagttt tagaattaac gaatgctgtt tttcaaaaga
     1501 aattttttca gaaatcctaa aaattggcat acctacatta atatttcaat tattgactag
     1561 tcttgccatt acattaacta atatggaggc tacaaagtat ggtgattctg tgatagctgg
     1621 aatgggtgct gtaacacgaa taacatcttt aggtagtctt gttgtatttg gttttataaa
     1681 aggtttccaa ccaattgctg gttataattt tggttcgaag aactacgata aattacatat
     1741 agcaataaaa acttccatat tatggactac aatattttgt gtagtgcttg gtcttatatt
     1801 ggtgcttttc ccaaaatcta taatttcaca atttacaagt ggtgacattc tattaataga
     1861 aaatggtagt aaagctctta gggcaaatgg gttttcattt atgctatttg gattctatac
     1921 agtgtattct acattattcc tagcactagg aaaagcaaag gaaggtggat ttttaggtat
     1981 gtgtagacaa ggcgtctgtt ttatccctat aattttgatt attcctgtat tctatgggtt
     2041 aaatggtatc ttatatgcac aaccaatagc tgatttgatg gctgctataa ttgctggttt
     2101 tatgtctata aggttacata aggaacttaa tcttttaaaa ataaacggta tgcattagtt
     2161 atacaaacct actatcatca aaacatatta ctttctcatt aatagcaaga atatatatta
     2221 tcatattttt tctacataaa tcatactatt actatctgac taaataatcc tcttaacaaa
     2281 ctacttgtca atttatctat tcacttgtat catgggatta taacaattta agaaaggaga
     2341 tattgtatga tttcacaaaa tgaaattgat atacttacac atagcttgcc tttttgggat
     2401 aaacttactg acttacaaaa agaattatta atttcttcag caaatacctc ggattacaaa
     2461 aaagggaatc cagtacattg tggtgatagt gactgtattg gtattttgat tataaagtct
     2521 ggaacaatac gtacatacat actttcagat gaaggtcgag aggttacact gttccgactt
     2581 gatgatggag atgtatgtat tttatcagct tcctgtattc taaaaacaat aacttttgat
     2641 gtctatgtag atgctgaaac ggactgcgat attatacaga ttaattcctc tgtttttgca
     2701 aaattatcta cagaaaatat tcatgcagag ttattttcat ataaattagc taccgaacgt
     2761 ttttctgatg ttatgtgggc tatgcaacaa attcttttta tgagttttga taagcgtttg
     2821 gcatcttttt taattgatga gatagctaaa aatggcagta gcactataaa tatgactcat
     2881 gagcaaattg ctaagtatat gggtagtgcc agagaagttg tatcgagaat gttgaaatat
     2941 tttgctagag aaggtattgt gtctttatct cgtggtggta taaaagtatt ggataaggat
     3001 aaacttcgtt ctttgacact gtaaaagtgt gtctcaaaat gaacttttta gttcatgagg
     3061 cactcttttt tgtgtgaaat caaatatatt ttcataattt caataataaa aaagatactt
     3121 atatctattt ttatatagta tcttatttga tatttacatt tattttttat gtatattttt
     3181 atattttaat aatttagttt aactattttt ctatgctatt tatatagaat atttctaata
     3241 actcataaat tctaatcttt taatatcta
//
LOCUS       DAEPOZ010000309         3221 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.310, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000309 DAEPOZ010000000
VERSION     DAEPOZ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.310"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            131..792
                     /locus_tag="KR440_003457"
                     /pseudo
     CDS             131..792
                     /locus_tag="KR440_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            complement(1011..2048)
                     /locus_tag="KR440_003458"
     CDS             complement(1011..2048)
                     /locus_tag="KR440_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBH0700459.1"
                     /translation="MNKKLIKAKIFIWSILAISLSIVFISLLIGTIISHSFNFPFNTL
                     NPKYNYDNYIVAKNESFSDKINDINVDWISGEVRIKRSDSNKIKLIQKIPSNFSKKKL
                     AKIMVDGNSLSIYDNSTKKFFIGINLPKPTILELYLPNKLYDKITLYTTSADIYSTYI
                     RSNTVEANTISGAIGLSGNINKINLDTTSGHIDITKLNSKNATLKSTSGEIQLSGKID
                     VLNLETTNGNINLKDIYNKNLTCSSVSGKMNLSGVFSDIKAESTSSNIKINSSKMLSS
                     LYCETTSANVDLSIPDNPGFTLNFDKVSGVLDSDFELNRHSDLYTYKNGITNLYLSTT
                     NGNLTIIKNNH"
     gene            complement(2041..2727)
                     /locus_tag="KR440_003459"
     CDS             complement(2041..2727)
                     /locus_tag="KR440_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700460.1"
                     /translation="MLDKIRTYIDSSFTGVNETKKVKELKDELFENLKEKYNDQIQDG
                     KTEQEAYNSVISGIGDLSELIESVKEPYSLPSELIEEKKKRALRASIAVALFIISPFL
                     FVVSVVSFGIEPTIAILLMLILIALGVGLLVYNQMTRTNYALYNDNLSNKFKEFEDFE
                     ENYNHINPVYKSFMNAYWIIVVSIYLITSILFNIWSFSWILFIIGAAGVNIVKGIIQL
                     KGYKEEDKYE"
     gene            complement(2739..3059)
                     /locus_tag="KR440_003460"
     CDS             complement(2739..3059)
                     /locus_tag="KR440_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0700461.1"
                     /translation="MIIASDMIRGHTETIILKHLLEKDSYGYEINKSILEKTKGLYEL
                     KEATLYTAFRRLEQSGAITSYWGDEVTGARRKYYSITNIGKEKYIKSREEWNNTKALI
                     DILI"
BASE COUNT         1148 a          409 c          465 g         1199 t
ORIGIN      
        1 tcaaaataaa taacttatgt aagaaccgta ggaactacgg ggatagcctg tggagaatta
       61 gtaagacata tgttagtatg caaaattcta tgaagcagga accctgtgac tttagtcatg
      121 ggaggttcag attttggctg aagatgaaga aaataatcat ataaatattg agatgcaaag
      181 agcttttgag aacgattatt tggaaagagc tgaatattat cttagtcgtg tacatggtag
      241 aaagttagaa gaaggtaaag agtataaaga aattggaaaa actattggaa tacatatact
      301 aaatcatgta aaatataatc acatagaaga ttatgtaaat tgcctgagat taacaatgga
      361 cgagcatcca gatatttatt ctagtaaaac tgcattatat tttatagagc ttccaaaaat
      421 tcataaatct gactatatag taaatagaat tatgctatgg ggaaaactta ttgataatcc
      481 ttctagctca gatgtaagag ttatagctaa gaatgattct attattaaaa aagcacttga
      541 tagattaaaa gaattaggta gcaatgaaga atatttatta aatctgaaaa gaggtgctta
      601 tatcatgaat aaaagtagaa attttaaaga agagattgaa agagagacag caattagagt
      661 tgcaaaagaa aaagattata aaataattat aatgttgaaa aaaatggttt taaattagat
      721 gacatattga ataaatttaa tgattttgac ttaagtgaag atgagataaa agaagtatat
      781 gaaaataatt gatgataaat aaaaagtgaa cttatataag tttgctgaat ctgagcctgg
      841 aaatatttat gtgtattttt taatgaataa aaatttattt taaaaacaat aattagaggt
      901 acagttaatt atatttttat gatactgtac ttctagtttt ttctattgta gatttattat
      961 ttactaataa atctacaata gaagtgcttt tgttgtaaga atcattttat ctaatgattg
     1021 ttttttataa tagttaaatt tccatttgta gtgcttaaat ataaatttgt aataccattt
     1081 ttataagtgt ataaatcact atgacgattc aactcaaagt cactgtccaa aacaccagaa
     1141 accttatcaa aatttaaagt gaaacctgga ttatcaggaa tagacaaatc gacattggca
     1201 gaagtagttt cacagtacag agaagacaac attttgctag aattgatttt tatattactg
     1261 ctagttgact cagctttaat gtcagaaaaa actccagata agttcatttt tccagaaact
     1321 gaactacaag taagattttt attgtatata tcttttaaat ttatattgcc attagttgtt
     1381 tctaagttta aaacatcaat tttcccagaa agctgtattt caccagatgt tgatttgagt
     1441 gtagcatttt tactatttag ttttgttata tcaatgtgac cactagttgt gtctaaattt
     1501 attttattaa tattaccaga gagacctatt gcacctgaaa ttgtgttagc ttcaactgta
     1561 ttacttctta tgtaagtgct atatatatct gctgaagtag tatataaggt tattttatca
     1621 taaagcttat tgggcaaata tagttcaagt attgttggtt ttggaagatt aattccaatg
     1681 aagaattttt tagttgaatt atcatatata gaaagagaat ttccgtcaac cattattttt
     1741 gctagttttt tctttgagaa attactaggg attttttgaa ttaatttgat tttattagaa
     1801 tcacttctct taattctaac ttcacctgaa atccaatcaa cattaatgtc atttatttta
     1861 tctgaaaaac tttcattttt agccactata tagttatcat aattgtattt aggattaaga
     1921 gtattaaaag ggaaattaaa agaatgtgaa attatagttc ctataaggag tgagataaat
     1981 acaatagaaa gtgatattgc caggatactc cagatgaaaa tctttgcctt tataagcttt
     2041 ttattcatat ttatcctcct ccttgtagcc ttttagttgt ataattcctt tgacaatgtt
     2101 gacaccagca gcacctatta taaatagtat ccacgaaaag gaccaaatat taaatagtat
     2161 gcttgtgatt agatatatag aaactactat aatccaatat gcattcataa atgatttata
     2221 aactggattt atatgattat aattttcctc aaaatcctca aattctttga atttatttga
     2281 taaattatca ttgtatagag catagtttgt ccttgtcatt tgattataga ctaataaacc
     2341 tactccaagt gctattaata tgagcattaa aagaatagct atagttggtt ctatgccaaa
     2401 acttacaact gagactacaa ataggaaagg gcttattata aaaagagcaa ctgctattga
     2461 tgctcttaag gctcttttct ttttttcttc aattaattca gaaggtagag aatatggttc
     2521 cttaacactt tcaattaact cagataaatc accaattcct gagattactg agttatatgc
     2581 ttcttgttct gttttaccat cttgtatttg gtcgttatac ttttctttta gattttcaaa
     2641 taattcatct ttaagttctt tgactttttt tgtctcatta actcctgtaa agctactgtc
     2701 aatataagta cgtattttgt ctaacatatt ttcacctcct aaattaatat atctatcaat
     2761 gcttttgtat tattccattc ttcacggctt tttatatatt tctctttacc tatgtttgtt
     2821 attgaataat actttcttct agctccagta acttcatcac cccaataaga ggttattgca
     2881 ccagattgtt caagtctacg aaatgctgta tacaaagtag cttctttcaa ctcatacaat
     2941 cctttggttt tttcaagtat agatttatta atctcataac cataactgtc tttttcaagt
     3001 aaatgtttta agataattgt ttctgtatga cctcttatca tgtcagatgc gattatcatg
     3061 aaaatcacct ccaattaaat atataatact ttgatatact tcgcctgtca aggtatatca
     3121 tgagaaaaga cattttattg taatatttat aaggttttat atagaaataa tgtaaaaact
     3181 ttataaataa tatattttat acctagttat agataacttt a
//
LOCUS       DAEPOZ010000310         3217 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.311, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000310 DAEPOZ010000000
VERSION     DAEPOZ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.311"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(59..352)
                     /locus_tag="KR440_003461"
     CDS             complement(59..352)
                     /locus_tag="KR440_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700462.1"
                     /translation="MISMIMMLIGVLFIVFGIILTAVEIKGKMPGFYGVGQNAVRKNA
                     IGIIAAGIALIFFRNGLDSIDSILTYGVFLVIELVVIGLYFLIRIKYDKDGNL"
     gene            complement(836..1039)
                     /locus_tag="KR440_003462"
     CDS             complement(836..1039)
                     /locus_tag="KR440_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700463.1"
                     /translation="MSFGKRIEMNNIGVETRGVCTIMCPYTCKMMASSSCNCKNNDYN
                     ARGGSYNTYYNKLIHGQRTPDGM"
     gene            complement(1090..1341)
                     /locus_tag="KR440_003463"
     CDS             complement(1090..1341)
                     /locus_tag="KR440_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide maturation system acyl carrier-related
                     protein"
                     /protein_id="HBH0700464.1"
                     /translation="MNYTEKELLLENIFFDVTGINFNKNNNLKKELFFSPSLHIQPRE
                     LLLVFCEVIENFNIKIPEEKLLNRQFTSFDNVLKIINMV"
     gene            complement(1357..2427)
                     /locus_tag="KR440_003464"
     CDS             complement(1357..2427)
                     /locus_tag="KR440_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04066 family peptide maturation system
                     protein"
                     /protein_id="HBH0700465.1"
                     /translation="MEKVLIYPFNYNFFTILNHCNKISDIEINSLVSPSGWGLCNKEY
                     NVNNIITKVTNDFQSELKKNSLVWFVDSKENLDLEKFIIPKLKVALKEGKKILYTRDD
                     LNKISRYISHYEFKKINEWNLEKYEIRDIEGISQINTPIIFICGLYGGLDTFDVNIRI
                     GEELRKRGFSILQFGPKKESYLFKMIPIPEFMFDDSISSKTKIINFNHFIKDKEVEAK
                     PDLIIIEVPGELFPFSRKMVGNFGIRAFEISNAVINDCGILCIPSSIYDREYCEELKY
                     FIKGRHGINIDYFSIEKKVIDTYETEVKQKFSYMSLDENFFVEDIEDYEKTFFIHDNK
                     QLLELVDNIVNQLKEYAKVISI"
     gene            complement(2456..>3217)
                     /locus_tag="KR440_003465"
     CDS             complement(2456..>3217)
                     /locus_tag="KR440_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-rich peptide radical SAM maturase CcpM"
                     /protein_id="HBH0700466.1"
                     /translation="KFANTGEGSHDVVLKNVRYIKNKYPEYYKKNITFNTVINSENDY
                     ISIDDFISKNETLKDSNFLASTLNNNYSDKEIVSSDEYILGKSYDRFLTLLNKLKFIK
                     DESMSPLSRIERALLDDFRYEKNIEGRLELPDVWHHGGPCIPGAFRIFVTTDGEFFPY
                     ERVSESSKMGMIGNLDSGVDIEKAEKLLNIGKLYEEQCSDCWAYSYCDICIAKIDDTK
                     CNENEIKPHDCDRIRHSTENNMKDYCVLKSLGYQY"
BASE COUNT         1141 a          530 c          248 g         1298 t
ORIGIN      
        1 tatctacaat ttttttatat aaacatatat caaaatcaaa atattattaa tttatacttt
       61 aaagattccc atctttatca tattttattc ttataagaaa atataatcct attactacta
      121 actctatcac taaaaatact ccataggtaa gaattgaatc aatactatct aacccatttc
      181 taaaaaaaat taatgctata cctgctgcta taatccctat agcatttttt cttactgcat
      241 tttgcccaac tccataaaaa cctggcattt ttccttttat ttcgacagca gttaaaatta
      301 tcccaaacac tataaataat actccaataa gcatcataat catactaatc atctaattac
      361 ctccttcact taaggaaatt tcaatttcta aaatctaaaa taaataatat ttatttttta
      421 taataatatt gtatatccaa ttaactattt tggaaattag taccaataaa taatgtttaa
      481 atattgaaat aatatattaa ttctaatcaa tattattaat atatatacat ttttacaaat
      541 atatttaaaa actttacttt taattcttac tatttaaata agtataattt attataataa
      601 cttttaatta atttatactt ttaaattgta aaaatcaccc ctaatcacta tatttaccac
      661 tataataatt gaagaggtga tttatataac agatactatt tatgcagtta ttataaaatc
      721 acactcttaa ttataatcaa ctgcatattt ttataataaa aggaaataaa gtaagaaaga
      781 taaatttaat caaatatgct aaatcaattt aatagtatac ttattactga aatatttaca
      841 ttccatcagg agttctttga ccatgtatca atttattata atatgtatta tatgatcctc
      901 ctcttgcatt ataatcatta ttcttgcagt tacaagatga agatgccatc atcttacaag
      961 tataaggaca cataatagta caaacacctc ttgtctcaac acctatatta ttcatttcta
     1021 ttctttttcc aaaactcatt catatcctcc ccttttctat aagatcactt actttatacc
     1081 tttattaacc tataccatat tgattatttt tagaacatta tcaaaactcg taaattgcct
     1141 attcaaaagt ttttcctctg gaatttttat attaaagttt tctattacct cgcaaaatac
     1201 taaaagtaat tctcttggct gtatatgtaa agacggtgaa aaaaataatt ctttttttaa
     1261 attgttattt ttattaaaat taattccagt cacatcaaaa aatatattct ctagtagtaa
     1321 ttccttttca gtgtaattca taattaacct ccaattctaa atagatatta ctttagcata
     1381 ctcttttaat tgattcacta tattgtccac taattctagt aattgtttat tatcatgtat
     1441 aaaaaatgtt ttttcataat cttctatatc ttcaacaaaa aagttttcat ctaatgacat
     1501 atatgaaaat ttttgcttca cctctgtctc atatgtatct ataacttttt tctctatact
     1561 aaaataatct atatttatac catgtcttcc tttaataaaa tatttaagtt cttcacaata
     1621 ttctctatca tatatacttg atggaataca taatatacca caatcattta ttactgcatt
     1681 agaaatttca aaagctctaa ttccaaaatt accaaccatt tttctagaga aagggaataa
     1741 ttctcctgga acttctataa ttattaaatc tggctttgcc tctacctctt tatcttttat
     1801 aaaatggtta aaattaataa tctttgtctt actacttatc gaatcatcaa acataaactc
     1861 tggtatagga atcattttaa ataaatagct ctctttttta ggaccaaatt gtaatattga
     1921 aaaccccctt tttcttagtt cttctcctat ccttatattt acatcaaaag tatctaaccc
     1981 tccataaaga ccacaaataa atattattgg tgtgtttatc tgtgatatac cctctatatc
     2041 tcttatttca tatttttcta aattccattc attaatcttt ttgaattcat aatgagatat
     2101 atacctagat attttattaa gatcatcacg tgtatataat atctttttac cttcttttag
     2161 agctactttt aatttaggaa ttataaattt ttctaagtct agattttctt ttgaatcaac
     2221 aaaccatacc aaagaattct ttttaagttc actttgaaaa tcatttgtaa ctttagtaat
     2281 aatattatta acattatact ccttattgca taacccccag ccagaaggac taaccagtga
     2341 attaatttct atatctgaaa ttttattgca atgatttaaa atagtaaaaa aattataatt
     2401 aaatggatat ataagtactt tttccataat cccatctttc ctttcattag aaaatttaat
     2461 attgatatcc tagagatttc aaaacgcagt aatctttcat attattttct gtagaatgtc
     2521 taattctgtc acaatcatgt ggttttattt cattttcatt acatttagta tcatcaatct
     2581 tagctataca tatatcacaa taactatagg cccaacaatc agaacattgc tcttcatata
     2641 attttcctat atttaaaagt ttttcagcct tttctatatc tactccacta tctaaatttc
     2701 caatcatacc cattttagaa ctttcactta ctctctcata tggaaaaaat tctccatctg
     2761 tagttacaaa tattctaaaa gcccctggta tacatggtcc accatgatgc cacacatctg
     2821 gtaactctaa tctaccttca atatttttct catatctaaa atcatctaat aaggctcttt
     2881 ctattcttga taaaggtgac atactctcat cttttataaa ttttaatttg ttgagtaaag
     2941 tcaagaatct atcataactc ttacctaata tatattcatc tgatgaaact atttctttat
     3001 cagaatagtt attattaaga gtggaagcta aaaagtttga atcttttaaa gtctcatttt
     3061 tagatataaa atcatctata gaaatgtaat cattttctga atttatcact gtattaaatg
     3121 taatattctt tttataatat tctggatatt tattttttat gtaacgtaca ttttttaata
     3181 caacatcatg agatccttct cctgtatttg caaattt
//
LOCUS       DAEPOZ010000311         3212 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.312, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000311 DAEPOZ010000000
VERSION     DAEPOZ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.312"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            289..1662
                     /locus_tag="KR440_003466"
     CDS             289..1662
                     /locus_tag="KR440_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700467.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIVMYIFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(2035..2973)
                     /gene="pdaA"
                     /locus_tag="KR440_003467"
     CDS             complement(2035..2973)
                     /gene="pdaA"
                     /locus_tag="KR440_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBH0700468.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDGGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT         1047 a          411 c          528 g         1226 t
ORIGIN      
        1 aattgtgaga aaatagttgc atatcataat ggaagcataa atgtaaattc agatgataaa
       61 gaatttgcta ttgaaatatc tattccagca aaagaatcat aaaaatttaa attatatatt
      121 gtgtaattgc tattatatat tgtgtaattg cttatgtaaa attctatgaa gcaggaagca
      181 tgtgacttta gttatgggat gttcaatatt gtgtacctct aggcaagtaa caagtttact
      241 tacaagacca tataatatta ttatcttaaa aaaaggggac ataataatat ggttttgaca
      301 ggagcacatt atatttacat tctatttatg gtaattatat taattactat gattatgaaa
      361 aaggatacaa tagtaccatg tatattaggt gtttttttta tgggattgtt ctttgaaaaa
      421 aacatctttg gtgctataac agcagtgttt aactctttta ttatatcttt aaatgagttg
      481 ggtccaatta tcttgattat agctattatg gttgcattat ctaaagccct agaagccaat
      541 aatgctatac agtatatggt gagaccattt tcgagagtta taaaaaattc taatacagca
      601 ttttttgtaa ctggatttgt tatgttagtt ctttcttggt ttttctggcc aactccagct
      661 gttgccttag ttggagctgt atttttacct gttgctatga gagctgggct accagcaatt
      721 ggtgttgcag tagcattaaa tttatttggt catggtcttg ccttgtctac agactttgtt
      781 attcaaggag ctccaagtat aacagctggt gcagcaggtg ttgcagtatc atatgttata
      841 aatgatggta tgattttgtt ttgggtgatg ggaatcgtaa ccatatctat ggcattttat
      901 acattaaaaa gagatattaa caaaggaatg tttagagaag aactaaaagg ttttgaaagt
      961 gaagaagtag aagagttcaa tggaaaatct aaaatagcaa ctattttagt agctctggga
     1021 tttcttgctg atataattgt tatgtatata tttgacctaa aaggaggaga tgcatcagct
     1081 cttttgggtg gaacagcagt atttttaata ataattataa atgttataaa ttttggcaaa
     1141 gatagtcttg aaaatgtatg tgagaatata attgatggtt ttgtgtttgg gataaaaata
     1201 tttggagcaa ttataccaat agcagcattt ttctatatgg gtgaagttgc gcccttgaca
     1261 ggtgtatttg gaaaagtatt agcaccaggt tcacaaggtt tattatcaga tataggcata
     1321 gctctttctc aaacaatacc acttaataaa tttgccgtat caggtataga aactgtagtg
     1381 ggggcaataa ctgggcttga tggttctgga ttttctggga tttcattagt tggttcactt
     1441 gcctctgtat ttggaactgc tataaatgca agtgttggtg cattagctgc attaggacaa
     1501 atcagtggta tatgggtagg tggaggctgt ctagttcctt ggggtcttat ttcagcagcg
     1561 gcaatttgtg gggttagtcc aatagagttg gcaaagcgaa actttgtccc agttataacc
     1621 ggattggttg taacaaccat agtagcaatg tttattattt aataaatcga ataaatcttt
     1681 aattgtacaa attaaaaata gatgtgtagc tataatttat tataattgtt ttactaattt
     1741 taagccatac atctattttt attgatattt ttattagtat tctttaataa aataatattt
     1801 ttcattatac tatacataac atctaaatat aacatctaaa atactgttat ctatgtttaa
     1861 tatcttgcca aattttttga tgttttatta ccctgtccca ccaacatata taagctatcg
     1921 caagtttttt acctatacta tcaccaaaca aaaattttaa ttttaattta ttattttata
     1981 atctatatta tattaactat attagtttgt agactttaga tgtaattcta aatattaata
     2041 ctccaagagt tcaaattcat atcctagttt tcttgcattt gatataaaat catttaaaat
     2101 ttctgtacta gttttggaaa ctgcatgtag taacaagata gaaccatcat gtagattatc
     2161 cattattttt tgcgttgctt cttcatgtga tggttgttta tcagtatccc agtctctgta
     2221 tgcaaaactc caaaatacag tcttgtatcc taaattttta gtcattgcca agcttttttc
     2281 tgagtatttt cccataggag gtctaaaaaa tttaaccata tctttcccag ttacatcttt
     2341 atataacttt tcaacatcat ataattcgtc attaaagttt tttagattgc ttgtttttgt
     2401 tggcatagat ggatgggttt tactatgatt tccaactata tgtccctcag aaaccattct
     2461 ttttactaaa tctttatttt cttttatata tggagctgta acaaaaaaaa ctgctttaac
     2521 ctggttttgt tttagagtgt cgagtatctt tgtagtgtat ccattttcat aaccttcatc
     2581 aaatgtaaga tatagtgttt tttgaccatc ttttgtaggt ccattgtata gggcatcata
     2641 gtcagaaaat ttaaattcaa tgtccgtatt tacttctggt gttttatgct tattgttagg
     2701 tatataaaac caatcaagag tagtgttatc taaagaagat acatctaatt tattattatc
     2761 attattagta gtggcttttg atggtttatc tgttccgtct gaaactatta cattggaagt
     2821 atccttacca ccatctattt tttccttatc ttcttgtaac tgtgtttcct tattttgatt
     2881 ttcgttttga ttgttttgtg aatttgaaca tccaacaata aaaaaaatag aaatagaaag
     2941 tagtgttaaa taatgcaata cttttctaaa cattttgaag ctcctttcta aattaataat
     3001 aattgcatat agtattatta taattggaaa ttactattaa taatagttat atctttaaaa
     3061 ataaagtcac aatagtatga caaagggtga ataatcaata aatttaaaaa aaaatgaaaa
     3121 aaatagttga ccatattatg agaagatgtt attataataa atgtgctcaa caagaagtaa
     3181 caaaataaca agcacaaaga agttaattga at
//
LOCUS       DAEPOZ010000312         3175 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.313, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000312 DAEPOZ010000000
VERSION     DAEPOZ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.313"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            174..806
                     /locus_tag="KR440_003468"
     CDS             174..806
                     /locus_tag="KR440_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBH0700469.1"
                     /translation="MTIPNSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVSPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
     gene            complement(1177..2046)
                     /locus_tag="KR440_003469"
     CDS             complement(1177..2046)
                     /locus_tag="KR440_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH0700470.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
     gene            complement(2143..3000)
                     /locus_tag="KR440_003470"
     CDS             complement(2143..3000)
                     /locus_tag="KR440_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0700471.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
BASE COUNT         1094 a          479 c          344 g         1258 t
ORIGIN      
        1 aactaaacat ataatcttta taaaatacta tacagttaaa agtctgtttt aaaaaaaaat
       61 gtataaatta taaatcaaaa tatagatact atcaaggagt gattctgtga agagatgtat
      121 tgtagtagtg gaatagtcaa aaacataata tatattaaag gagagattta aatatgacta
      181 ttccaaattc aaataaggta tatccaagaa gtaatgatta tcaaactgta tatcttaaaa
      241 atgtaataac aagggaaaat atagaagttg gagattacac aatatataat gacttttata
      301 atgacccaag agattttgag aaaaacaatg tattatatca ttaccctata aacaatgata
      361 aattaataat tggtaagttt tgctcaatag catgtaatgc aaaatttcta atgactagtg
      421 gaaatcactc aatgaaatca aaatccacat atacttttcc catcttttat gaagaatgga
      481 aactcgatat caaccatata acggacgctt gggacaataa gggtaacatt ataattggaa
      541 atgatgtgtg gataggttat gattctataa ttatgtcagg tgtaaatatt ggtgatggtg
      601 caattattgg aacaagagca gtagttacta atgacgtctc accgtattct attgttggag
      661 gagtacctgc aaaaattata aaaaagagat ttaataatga tacaatttca aaattattag
      721 aaattaaatg gtgggattgg ccatataaaa aaattcaatc aaacattaag catattcaat
      781 caggaaatat tgataaatta gtttaatatt ttataattta tttatattaa actttacttt
      841 aataatcctt ttataagagc tttttcaaaa tttgtatatt attttaatat atataaattt
      901 aatacaacat taaataaaat aaaaagtaaa gttacttaat aaaaagttac tttacttttt
      961 attatttaaa ttcctgtttt tattataata ggtaaaattc tattaaacta gttcacattt
     1021 tcattattat tgatatatta tatatgaata taccattttg ttttttaata aaaaaatcat
     1081 gctacatcag tagtaagcaa ctattaatgt agcatgattt tttgtatcta aataatctta
     1141 gtatttacct atcaatatag attgatatat caaaaactat agtcttctat attcttctct
     1201 ttttagcttt tggttactta gatagtaatc aatttcatca attagacgtt gtttatcatc
     1261 aggtaataca ccttttaaat tagcataatt tgatgcatga ttacttctaa atacaatttt
     1321 ttcattagca tctatattct ttatcatctc tttaatttct tgtaacacag ccttatcctc
     1381 tagtattgta aactctcctg ccttaagtaa atcgtaaagt tcagtattag gttctaccat
     1441 taaactaagt acacccacat aatctggtga aattgctcct aacatttttc ctgtatcaac
     1501 tgcatgctga tgtgtttttt tagttcctcc aagacctgct attacagtgg ctgataattt
     1561 aatcccactt tttttaactt ttctaccaac ttttattaat tgttcactac taaaaccttt
     1621 cttaatatct tctaatactt cattgcttcc actttcaagc cctagataaa tcattttaac
     1681 accaagtttt cttaattctt taagttcatc atcagatttt ttctctatag atttaggaga
     1741 gccgtacata gtaattcttt cacactctgg aaaaacttct tttatatata aaataatatc
     1801 tcttaatttc tccattggta ttatcaatgc atctccatct gctaagaata ttctttctac
     1861 atgcttataa tatcttctaa atatatcaat ttcatttttt attacttcta gtggctttat
     1921 tttaaatttt ttagatttgt acatactaca aaacgtacat ttgttatgag aacatcctag
     1981 tgtgacttgt attataagac tataagcctc acttggtggt ctgtaaagtg gcatatcgta
     2041 ttccatatta aaatccccct tgtaataaaa tattcattct attatattat taatatttac
     2101 tttaaataat aacttataaa attatatttt ttaaatttta gtttaagata tattctttaa
     2161 aaatttaatg atactattgg cagtaatatt aagttgtttt tcttgactta taatagcatc
     2221 attatctccc ttttgttttc catagtctcc aaattgtgca tgatttcctc cttcaatctc
     2281 aacataagtt gtatcatcag gaagtttttg ttttgattct acaagacttt taaaattgac
     2341 tactccatct ttgctccccc atatagatat aacttttttt cctaattctt tcaactcatc
     2401 acccattgga taagaagcta aaaatacaac gccttcaatt aatttattat cacgtgcaaa
     2461 atttgatgct gcaactcctc ctaatgaatg acctcctatt acccaatgtt ctatgttgtt
     2521 gtaagaatcc ataactttct gtgccttatt agttccaagt atagctagat taagtggcat
     2581 ctttacgatt acaacctcat atccactttc tgctattttt cttgcaagtg gagcataaga
     2641 ttctatttct actttagctc ctggatacaa aattaaacct ttagaaactt ttcttccttt
     2701 tggtgtaaat gaaatgtttt catttacagt tacttcaact ttactattgc tgactagagc
     2761 ttcttttgcc agcttttgag gttcatatgt tttatttatc catatgaaaa agccaagcaa
     2821 aatcaataca aatagtatac ctattccctt aaatatctta ttctttatgt taaattttct
     2881 attattattt ttatcatctt tgttctcaac ctcatcataa agtaaaggtc tctcaagttc
     2941 catagttata ctaaatgtat catatttctt attacgcttt acctttacat ctttactcat
     3001 aaatctaacc cccataatat ttgaatacta ttatagttta tcctaagtaa attgtttttt
     3061 caaccaatga aaaaaatcaa tattttaact attttcatat aaaattcata aatatatgtt
     3121 ttttgttttt tatatattat tttcttttag tattttataa aacaaatagc catat
//
LOCUS       DAEPOZ010000313         3154 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.314, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000313 DAEPOZ010000000
VERSION     DAEPOZ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.314"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     repeat_region   173..466
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=240..268
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(1127..>3154)
                     /locus_tag="KR440_003471"
     CDS             complement(1127..>3154)
                     /locus_tag="KR440_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0700472.1"
                     /translation="HTNEIIFMNKKMKEEYNILDPEGKVCWQVLYPEQNSTCSFCKVL
                     ELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQDGTVVHMHQSIDIANSTILNKPI
                     KINEFNEISNTKEGKGVFNFSRDNFDYNSTLLYDALIRGTDEYIYICNMKTGVFRYSP
                     SQVELFDLPGEIVENPLIYWKKIVHPEDWNRFYKSNTEIGKNQMDYHTVEFRAKNRSG
                     EYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKIDSLTQLLNYHEFMSVFEDKISNP
                     MIEKLCIVLLNIDDFKNVNEMYDRDFGDNIIKTLAQSIQSILPDNAELYKLDGDEMGI
                     LVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITISAGCVIYPKHGDTVKELYKCAS
                     YSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLKASINDDFRGFSLRFQPQVDTQS
                     HKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMINIVGAWVLRMALRTFRKWIDYYP
                     FFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNLVLELTESHTVQNMSILQFKFKA
                     LQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRVFVKDILKSKFDATFIHFIVAIC
                     HDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGKPQTATEIFDLLKLDN"
BASE COUNT         1110 a          496 c          308 g         1240 t
ORIGIN      
        1 tgtaattatg taagctattt ataataaaac actgataaaa ttaataataa aaaggtaata
       61 ggatttctat actacctttt ggtttttata atatagtgta attttattat atttttgtat
      121 gctaatgtaa ctagataatc ataagataac acattaaaag aaaattataa aaatttatat
      181 tccgtatagt tagtataaaa cgtaagtaat ataaatttat gtaaaacaac aaaagatgga
      241 tttacattcc atatagttaa tataaaacgt tttaatgaat taacagaaga tgaaagtttt
      301 atatgtattt acattccata tagttaatat aaaaccattg aaaatagtga cttttacgaa
      361 aaatttgcta aaatttacat tccatatagt taatataaaa cacttcctat taaatccaca
      421 ttaggaaatt ctccgagatt tacattccat atagttaata taaaacccca aaataaactt
      481 agtattttca acacttacac gtatacaact ctcctaaatt tgcagtgaac gagcaatagt
      541 gcaattgata acgcttatca tgcactctca acaccttata tttcaactgt tacgccaaat
      601 tttatcacaa atatagctca ctgcaaaatt tctataattt tattatatca taaatatatt
      661 acttttgaat attgatacca atttatacca taataaaagc aagaaaaaac gtaatctatc
      721 tacattggat ttaaattcta taacttaaag ttttttacat aatatttatg cttgtttaat
      781 caatctaata tatcattctt gtatattgct tttaatagtc ttttttctta aataaatatc
      841 tatctgaaca agcatagttt aattaaaaac ttttgtattc ccttaaaact gtatctacct
      901 cttattattt tatttatgat atattatttt taatctaaaa atagatatct atttttatta
      961 tagttatccc tttttaaaat actttaatca tatactactc ctaatttaac atcccttgaa
     1021 atgctatttt cttatattgc aatttataat tgatatatct tcaatataat aatttaatgt
     1081 atttttgatt ttaaagcttt tcagtgatat tattgataaa ttagaattaa ttatctagct
     1141 ttaaaaggtc aaagatttct gttgctgtct gcggttttcc aaataaatat ccttgtatgt
     1201 aatctggctt tatttgttta actaaatcat actcttcttg tgtttctaca ccttctaaac
     1261 atacttttat tcctacatca tgacaaattg caacaataaa atgtataaat gtagcatcaa
     1321 acttactctt taatatatct ttaacaaata ctctatcaat tttcactata tctattggag
     1381 aaaattttaa aacttctaga gacgagtatc cagtaccaaa atcatccata gcaatataaa
     1441 ttcctaaatc ttgtaaagcc ttaaatttaa actgtaaaat actcatattt tgtactgtat
     1501 gactttctgt caattctaaa acaagatttt gataaggaaa attttcatca tctataattt
     1561 ttactatatc ttcaataaat gtgtcttcta atatttgaac agctgaaacg tttacactca
     1621 ctttaaaaaa aggatagtaa tcaatccatt tcctaaaagt acgtaatgcc attcgaagca
     1681 cccaagcccc tacaatattt atcatatcat tttcttctaa tattggaata aattccaaag
     1741 gagaaattgc tttgcatttg tcattagtcc accttaaaag aacttctaca cctattattt
     1801 tatgactttg tgtatctact tgtggctgga atcgaagact aaatccccta aaatcatcat
     1861 taattgatgc ctttaaatcc cgcatcattt ctaatgaata cattttattt tttaatattt
     1921 cttgggagaa aaaaacaagc ctgtttttac catgctcttt cgcatattgt aaagagtaac
     1981 tagcgcattt ataaagttct tttactgtat ctccatgttt tggatatatt acacaaccag
     2041 cagaaattgt tatatttaat ccatattttc tccatagttg aaggtgtata atcatattct
     2101 gtatttgatt atatagagtt agaatttcat tttcttcaac attatcaaca agaataccca
     2161 tttcgtcccc atcaagttta taaagttcag cattatctgg taaaatagat tgtatagact
     2221 gtgctagtgt ttttattata ttatctccaa aatccctatc atacatctca ttaacatttt
     2281 taaaatcgtc aatatttaat aatactatgc ataatttttc aatcatagga tttgaaattt
     2341 tatcttcaaa tacactcata aattcatgat aattcagtaa ctgtgtcaat gaatcaatct
     2401 tattttgttt acctaattgc gtcattattc ctgcaaaaat acttggttct ccaaattcat
     2461 cacgcattag ttgtcctcta catcttaacc atatatattc tccactacga tttttagccc
     2521 taaattccac tgtatgataa tccatctggt ttttacctat ttcagtgttt gacttataaa
     2581 aacgattcca gtcttctgga tgtacaattt ttttccagta aataagcgga ttttcaacaa
     2641 tttctcctgg taagtcaaat aattcaactt gtgatggtga atatcgaaat acgcctgttt
     2701 tcatattaca aatgtatata tattcatctg tacctctaat caaagcatca taaagcaatg
     2761 tagaattata atcaaaattg tctcttgaaa aattaaaaac tccttttcct tctttagtat
     2821 ttgatatttc attaaattca ttaatcttaa ttggtttatt taagattgtt gagtttgcaa
     2881 tatctattga ttgatgcata tgaacaactg tgccatcttg ccatgtaatt aaactatcat
     2941 aattttcaaa aacacgattt aacttattac atttttcata ccactttatt aatactcctt
     3001 ttttatcatt ctttaatagt tctaaaactt tacaaaaact acaagtagaa ttttgctcag
     3061 gatataatac ttgccagcaa acttttcctt ctggatctaa aatattatat tcttctttca
     3121 ttttcttgtt cataaaaata atttcattag tatg
//
LOCUS       DAEPOZ010000314         3141 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.315, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000314 DAEPOZ010000000
VERSION     DAEPOZ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.315"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(98..277)
                     /locus_tag="KR440_003472"
     CDS             complement(98..277)
                     /locus_tag="KR440_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBH0700473.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDNSYDIVFSCIVFH
                     HCTNVQNIFCIFWKW"
     gene            complement(502..1035)
                     /locus_tag="KR440_003473"
     CDS             complement(502..1035)
                     /locus_tag="KR440_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBH0700474.1"
                     /translation="MQYISYYHSPIGNILLASDNVGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGEEPNFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     HKVAQQKGISKMSAQAIGGAVGHNGISIIVPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            complement(1853..2425)
                     /locus_tag="KR440_003474"
     CDS             complement(1853..2425)
                     /locus_tag="KR440_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBH0700475.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGSCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(2487..2750)
                     /locus_tag="KR440_003475"
     CDS             complement(2487..2750)
                     /locus_tag="KR440_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBH0700476.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDLYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            complement(2750..2971)
                     /locus_tag="KR440_003476"
     CDS             complement(2750..2971)
                     /locus_tag="KR440_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBH0700477.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYSNPKNRVRAKKGGNMY"
BASE COUNT         1065 a          521 c          361 g         1194 t
ORIGIN      
        1 agcatataaa tttcaacctt atcttgtatg gttcttaaca cttaatgagt agcttaagta
       61 tcaatcatta taaattttcc ccttatattt aacactctta ccatttccaa aatatacaaa
      121 atatattttg aacatttgta caatgatgaa atacaataca tgaaaataca atatcataac
      181 tgttatctaa aaatagaagt tcttcaacat aacctttcac aaagaacata ttatctaagt
      241 attttttgtc tgctccttgt tttcctgttt gtagcatata aattactgca tctaaacata
      301 taactactca cacgaaaaaa gaaaataaac ttccaaaata caagctctaa aactacctct
      361 aaagttatat aatgtttact aggaataagg ttgtataaca tatagtttaa agaatagttt
      421 tttaaaattg caagttattg ctttcaaaat tagatgttta tatatcaaat aaatgattaa
      481 gaatttctaa atttaatggc tttataatgc actccctttc tttgggataa aataatgttg
      541 cacagatact ttttctaaag ataacaattt tatttttcta tcaattccac cagcataccc
      601 tgtcaaactc ccatttgttc ctacaacacg atgacatgga acaataatag agattccatt
      661 atgccctact gcaccaccga ttgcttgtgc tgacatttta gatattcctt tttgttgagc
      721 aactttatgt gcaatttctc cataagtgat tgttttacca tatggtattt gttgtaaaat
      781 tttccacact gtagtctgga aaggagtacc taccatatgg agtgatggtg taaaattggg
      841 ctcttctccc gaaaaataaa tatctagcca tttctttgtc tgctcgaaaa ttggcaattc
      901 tttttcttga tgttcttggt caagattata agcataatac ttttgattct caaaccaaag
      961 accagttaac ccaacattat ctgatgctaa aagaatattt ccaattggac tatggtaata
     1021 gctaatatat tgcatacaat tcctccttat attctgaaaa cttcactcaa atcattagac
     1081 acttcttttc tatctgccat tatgtaaaaa catactaata taatttcctt ctgactacac
     1141 tttcgttata atatctatcc tcatatcata ttagttttat atttacctga atctgttcat
     1201 cctttttttc ctttcccttt gatggatata aaattcatac atttaagctt cctactgtat
     1261 taacttttat agaagtgtat attataattt tatcactaaa tagacaaaaa aatatcgata
     1321 taacatcaag aaatatttta ctgagtttcg cttattaaaa gcaactgaat ttctaaaggg
     1381 tacagatgag ctaataagtg atattgatat aaaagttgtt tttcattaag taatctattt
     1441 tagtaaatgt tttaaagaaa aaacgggata ttctccaaaa gtgtacagaa atataaaaaa
     1501 agagatatga ttttttattt cttttgatta tccaaagagt aaataaaaaa tattgtaaaa
     1561 aacttactaa caacggcaat gctttgaact atggtaatac tttgaacttt tgattggact
     1621 ttttttgcta aaataatata tttaaatagc aaagtaatct gtttaatcaa taaaaaataa
     1681 gagataaaag aaatcaacta aaacctttac ctcttatttt gatagtttat cagagctaat
     1741 atttacactc tttttatttt tgatacttca ttaaaaatga tgcttattct cttttcattt
     1801 ttaatacttc attaaaattg atgcttactc tctattcact tttaatactt aattagaact
     1861 gatgcttaca ctctgtaaac tcataaacat ccattaatgc ttcaccaaat ctttgatagt
     1921 gaactacttc cctctcaccc aaaaatctca atatatcttt caaatcaggg tcatcagtta
     1981 aatttattaa ttgatagtac gtagccaatg ctttttgttc agcagccata tcctcatgta
     2041 aatcagttac cgaatcgcca aagacattaa tataacttgt agtaaagttt actccattag
     2101 aatctactgg ttgaagacca tgtccaaaat tagcataatt actaccaagt ccagcagcct
     2161 taagctcttc cggagtacaa ctgcctatta attgatataa cactgaagaa attatctcaa
     2221 catgggccat ttcctctgta cctatatcag ttaaaagtgc gcgagatttt ccagttggca
     2281 tagtatatct ttgttgaaga tatctagttg cagctgccaa ctcaccatta ggtcctccca
     2341 actgagtcat gatatatttt gccattctag ggtctgcttg tcttatgtta actggatgtt
     2401 ccagtgtttt ttgataaatc cacatattat cttactcctc cttaatatat atttaataat
     2461 aattctaatt ttaaaactta ataattttat ttataaaatg atttgtccca tggccaaggt
     2521 tcagatatcc atgaccaagg atattcactt gattcatatc caaaattagt taatgctcca
     2581 tatttagatt cataatcaca cacagcttgt gtaaatttat cgcagtatct attgtataaa
     2641 tcaagagctt tcttatccct tggattgtta tctaaatata aatttaattc tatacaggca
     2701 aattgatatt ctgaaattct ctctaaaagt tctcttctat ttaatgacat tagtacatgt
     2761 tacccccttt tttagctcta actctatttt taggatttga atatagccaa tcattatagt
     2821 tatttgactc tattaaatct aattcaggaa ataaagtacc tctttttaat gctgtttttg
     2881 gattgtatgt gtcatcataa atttgacgat tgatgtatgg tctagcaaat tcacaacaat
     2941 agtcttgttt atcctctttt aaatatccca ttaattatcc tccttaattt ttagaatttt
     3001 aaaatagcat tatttttaaa aattgtatac ttgttctata ttatagtcag tagttttaat
     3061 aggtctttaa taagattaaa taattaaata agtatctttt tttgttctta ttttacctat
     3121 aaaaattttc ctaattaatc t
//
LOCUS       DAEPOZ010000315         3125 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.316, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000315 DAEPOZ010000000
VERSION     DAEPOZ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.316"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..1387
                     /locus_tag="KR440_003477"
     CDS             <1..1387
                     /locus_tag="KR440_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBH0700478.1"
                     /translation="VNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNTV
                     FIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVFA
                     KAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAGG
                     KTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIKN
                     IAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKKF
                     ATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVID
                     RNYNLDLVKRSKLQSNIEDKNSSEVILSYTDYNVGDKVKLLDEDDFGIVYKPMDKFNN
                     VEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKALK
                     KIQKEIRENRK"
     gene            complement(1450..2073)
                     /gene="ytaF"
                     /locus_tag="KR440_003478"
     CDS             complement(1450..2073)
                     /gene="ytaF"
                     /locus_tag="KR440_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HBH0700479.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLELNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            2258..2902
                     /locus_tag="KR440_003479"
     CDS             2258..2902
                     /locus_tag="KR440_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0700480.1"
                     /translation="MEQHSKVGNFGDLLKVLLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQRPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
BASE COUNT         1339 a          323 c          450 g         1013 t
ORIGIN      
        1 tgtaaataaa aagtatatac aagagtttat aataagtaaa agaaatgata gatatgtaat
       61 accaattaaa tcttcttata aaaatgaagt taatggtact atattagata catcatcaaa
      121 aggaaatact gtatttattg aacctattag tgtttcaaat ttaagtacag agttaactat
      181 gctaaaagct gatgaaacta ttgaagaata caaaatatta tcttatttaa cagaacttat
      241 atttgagaag ataagtcaga taaaattaaa tatagagata ctttcagaat acgatatggt
      301 atttgcaaaa gctaagtata gtcaaaaaat aaaaggaatt actccaaaaa taaataataa
      361 tggctatata aaaataatta aaggtaaaca tccacttcta acaggagatg cagtaccttt
      421 agattttgaa ataggcaaaa attatagaag tcttataata acaggtccta atgcaggagg
      481 aaaaactgta acccttaaga ctgttggttt actaacttta atggttcaat gtggatttga
      541 tatttctgca aaagaaggta gtgaatttag tgtatttgaa aaagtttttg tagatatagg
      601 ggataatcaa agtattgaaa atgccttaag tactttttca tcacatataa aaaatattgc
      661 agagattatg agtttatcaa ataattctac tcttgttctg tttgatgaaa taggaagtgg
      721 aactgaacct aatgaaggag ctggtttagc aatatcatta ttagaagagt tttacaaaat
      781 gggatgtata actatagcat caactcatta tggagagata aaaaagtttg ctacattaca
      841 tccagaattt gaaaatgctg gtatgatgtt tgataaagaa acgttagaac cattgtataa
      901 gttaactatt ggaaaatctg aagatagtaa tgctcttttt atttctaaaa aaatgggaat
      961 aaaaaataga gttttagata gagctaaaga atatgtaatt gatagaaact acaatttaga
     1021 tttagttaaa agaagtaagc tacaatctaa tattgaagat aaaaatagtt ctgaagtcat
     1081 tttaagctat acagattata atgtagggga taaggtaaaa ttactagatg aagatgattt
     1141 tggaattgta tataagccta tggacaagtt taataatgta gaagtattgt ttaatgataa
     1201 ttttatttca gtaaatataa aaagattaca acttagcata aaagcatcag aattgtatcc
     1261 agaagggtat gatttagatg tattatttac tagttttaaa gaaagaaaat tagaaaaaga
     1321 tattcaaaga ggttcaaaaa aagctcttaa aaagattcag aaagaaatta gagagaatag
     1381 aaaataaata taaaaagcac aaaagaaagc aatctaaaga gaaaagttta tctttagatt
     1441 gctttcttat taacatatct tcataaatcc aatgactaac aagattaaac cacttaacca
     1501 agataagttt aaatttacgt ttttaacaaa catatttcct agtaaacaac caagtaatac
     1561 tgcaattata tttgatatta aagaaaatag aataacttgt atataattca cacctacaag
     1621 gccactacca aaaccgatag ctaatccatc taaacttaaa gcaagtgcaa gtgtaaatgc
     1681 ttcagcagat ttaagcactt tagatttact tgtatctaag acatcaggtt cgatacttac
     1741 attaaaattt aattcaaggt ctaacatatt aaaattataa ttctttgagt gtatagattt
     1801 tttcttaaaa taagatttaa aaaaactttc aattagtcta gcacttccta ataaaaataa
     1861 aataaagaaa catattaaca ttgttatatt ttgaggtaaa aaatctctaa ctattgtacc
     1921 tgcaaaaagc gatattgcta aaatagctga acaaactacg ctgataatta tatttgaata
     1981 taagggaact ttaactttat ttactccata tccaaagctt gtcacaaagg catctataca
     2041 aagagaagta actaataaaa ttgattcaat cacagtagat taacatcctt tctcttaaat
     2101 tttgtactta tattagttat agtattgtaa aaatggataa attgttacag ataatccaat
     2161 aaattgttga taaaatttca acatcataca acacctaaat aacaaaagaa tcatatcata
     2221 taatataaaa ccaatcataa taaagcaggt gaaaataatg gaacaacata gtaaggttgg
     2281 gaatttcggt gatttgttga aagttttatt agatgaaaaa gaattatcaa tgggagaact
     2341 aagtaaaaaa agtggaattg ataaatctac tatatctcgt atagctaata ataaacaaag
     2401 gccaaatata aatcatttag aaaagatggc aatacattta aatattaacc tagaagaact
     2461 gttgaaagct tcaggatatg aatttagaaa cagcaataat caaatattta atgcagatag
     2521 tgattttagt aactttgatg atattcttgg atttgcaaat ttaatcaatg ataagaactt
     2581 taatggaaat attgaaaaag agttaagtaa gtgtaagtta tatgtccaaa cagatgaagg
     2641 taaaaaatta ctttttgata attttaataa aaaaatagat tctattgaaa aacaaggtca
     2701 atttacagat agattaagac aaatgtatgc tgatttttgt acagatggac ttacaataaa
     2761 gaaatattta ttgattggaa gtatgctact ttactttgtt atatcaacag atgtgattcc
     2821 agacttcgtg ttcccaatag gatttatgga tgatttagta gcattaaata tagttacaaa
     2881 attacttaaa aatgataagt aagttttaga ataatattta atatataaat aaatcaaagt
     2941 atataaaatt tattattttt tatatgaaac aaattaaaag cacaatgaag tagattaaaa
     3001 atttaaaact aagaatctaa tatatatttt gtaactattt aagtatataa aataaaataa
     3061 tatccaaact aatacaaaat agtaataatt gttttgtact ggaggatata tatttatgaa
     3121 ttgta
//
LOCUS       DAEPOZ010000316         3107 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.317, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000316 DAEPOZ010000000
VERSION     DAEPOZ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.317"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            17..1114
                     /locus_tag="KR440_003480"
     CDS             17..1114
                     /locus_tag="KR440_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBH0700481.1"
                     /translation="MIVSISIYLTGCTTSDNGNNQSNNNDSLSSSISSDKNVKIHFID
                     TGNSDAILIQDGKTFTLIDGGDNDDEGLMVDYLNNQGVKDIKYLIATHSHADHLGGLD
                     SVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPSVPLENNKFYLEDSYFEVLNT
                     NGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILPKLEKVDLLKVAHHGSRSSSS
                     QEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGVKVHRSDECSDIIFESTGDGV
                     FTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNTFNSEEIVYFTPKGKSYHSIK
                     NCSGLSRSKKILSGTIEESKKNDPCDICYGK"
     gene            complement(1262..1897)
                     /gene="lexA"
                     /locus_tag="KR440_003481"
     CDS             complement(1262..1897)
                     /gene="lexA"
                     /locus_tag="KR440_003481"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBH0700482.1"
                     /translation="MYLDLTEKQVLILEFIKSQITLKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(2001..3107)
                     /locus_tag="KR440_003482"
     CDS             complement(2001..3107)
                     /locus_tag="KR440_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019595437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBH0700483.1"
                     /translation="MKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKDYFIYLKG
                     SVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINLFLGDYCS
                     RYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPIKLPKPQP
                     DAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGLRLNELRE
                     LNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLNDEEKDALF
                     LSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGFSIYDVQN
                     LLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
BASE COUNT         1064 a          450 c          453 g         1140 t
ORIGIN      
        1 aactttgata cttttattga tagtatctat atcgatttat ttaactggtt gtactacatc
       61 agataatgga aataatcaat caaataataa tgattctttg tctagtagta tatcaagtga
      121 taagaatgta aaaattcatt ttattgacac tggaaatagt gatgcaatat tgattcaaga
      181 tggtaaaact tttacattga tagatggggg agataatgat gatgagggat taatggtaga
      241 ttacctaaac aatcaaggtg taaaagatat taaatatctt atagctaccc attctcatgc
      301 agaccattta ggtggacttg attctgttgt caaaaacttt aatatagaaa atgtttttgt
      361 atcgaatggt tcagcagaaa caaagagtta tagggacttt ataaatgctt tagcaaataa
      421 gggtttatcc ccaagtgtac cgcttgaaaa taataagttc tatttagaag attcttattt
      481 tgaagttcta aatacaaatg ggggagatac tactaatgaa caatctctag tccttgtata
      541 tactaatggg aatgataaag ttttatttac aggtgatgct gaagaaggta ccgaaaaaga
      601 aatactacct aagcttgaga aagtagacct tttaaaagta gctcatcatg gttctcgtag
      661 tagttcatct caagagtttt tagataaagt aaatcctgaa tatgcggttc ttttagtagg
      721 caagggtaat tcatatggtc atccacatca ggagacaatg aataaattga aaaaaatggg
      781 agtaaaagta catagaagtg atgagtgttc agatataata tttgaatcaa caggtgatgg
      841 agtatttaca agttgtaaag atggaagtta taataaaggt gttagagaag atggaaactc
      901 tggaaaatca aacagtaata caagtaaaaa tgattctttt aaaaataaac aaaatacatt
      961 caattcagaa gaaatagtgt attttactcc aaaaggtaag agttatcact ctataaaaaa
     1021 ttgtagtggg ctttcaagaa gtaaaaaaat attatcaggc acaattgaag aaagtaagaa
     1081 aaatgaccca tgtgatatat gttatggaaa ataacaaaaa aacaaccaat ttatctataa
     1141 agcttctgaa ctttaaatca tttaaaaaat aaaataaatg cataaaaaaa ataagctata
     1201 atcaatagat aatacatttc atctatacga atagcttatt tttttaagta ttttttaata
     1261 gctatttaat aactctaaat acaccagtta caatacctac aatttcaact tctgagtcat
     1321 tgagcataat tggttccata aaatcatttt ctggctgaag tcttattaaa tttccttctt
     1381 taaaaaacct cttaactgta gcagattcac catttataag tgcaactact atctgagaat
     1441 ttaaggccgt attttttttg tctactacaa cgtaatcttc atctaaaatt cctgcattaa
     1501 tcatactttc gccttttact cttaatacaa aattgttact gccctttact aaactggcag
     1561 ggaatggaat gtattcctct atgttttgtt gagctaaaat gggttctcca gctgttattt
     1621 gacctactaa aggaagctct attatttctt gattagctcc agaaacatca tttactttac
     1681 tacgttctaa aacttcaata gctcttggtt tagtagggtc ctttcttatg tatcccagtt
     1741 tttcaagttt atttagatga gagtgcacag ttgaagtaga tcttaaccct acagcagtac
     1801 atatttctct tacagcaggt ggataacctt ttaatgtgat ttgagacttt ataaattcca
     1861 atatcaagac ttgcttttca gttagatcta aatacataac atcactcatt tccaaacata
     1921 ttttgttttt atttccatac actttatatt tttaattata acataatcca tttatatctg
     1981 caaacatttg ttcctgattt ttaatcgtcc tcaatccatt caaaattctt gactatatca
     2041 cgttttacat ttttcttatg agctgcataa agctgagtag ttgtgacatt atcatggtca
     2101 agtaagtttt gaacatcata tatagaaaat cctgtttgga ttagagaagt tgcagccgtt
     2161 gctcttaatt tatggggact atatccattg tctctagtag tatcaagtgg aatagatgta
     2221 tattttttta ccagtgttct tatagccttt gctgtaattc tcttattttg aagagacaaa
     2281 aataaagcat ctttttcttc atcatttaat agttcatctc ttgttctttc gttttgcaaa
     2341 taatctttta ttacatgttc acaagtatga tttataggca ttaaaacttc tttcccacgt
     2401 tttctatata ttttaaattc acctcttgaa aaattaaaag aggagatatt gagttctcta
     2461 agctcattta atctaagccc gtatgtaaca aataatgcta gtatagcttt gtcacgtaat
     2521 ttagtttttc tccaatatac cttttctttc tctgtaagcc cttcacctgt ttctactgat
     2581 tcaagcatct tagcaacttc atctatctca aggcgcttaa tagcatctgg ctgaggttta
     2641 ggaagtttta ttggattgaa tccatcagtt atattattat ctatctgaga gtttctgtat
     2701 aagaatttaa atagagtaga gatggatgat ttttttcttg ctaacgctct attgttattt
     2761 tcaaaaatca atgtgttttt ttctgtattt ttgtaatatc tactgcaata atcacctaag
     2821 aataaattta tatctcttga ttttattgta ttaaaatcat cttctgtaat atcttttaca
     2881 caatctgcat ttgttaattc tttggtttca atcaaatatg aacaaaaaaa attaatatct
     2941 tctaaataag caagtcgtgt agaaactgca acagaacctt ttaaatatat aaaataatcc
     3001 ttcataaata caggaagttt attttccaaa agtagacatt tttctatgag ttcattttct
     3061 ttgataacaa tattatcagg tttatctgat ttattatgta cttttat
//
LOCUS       DAEPOZ010000317         3082 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.318, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000317 DAEPOZ010000000
VERSION     DAEPOZ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.318"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            169..597
                     /locus_tag="KR440_003483"
     CDS             169..597
                     /locus_tag="KR440_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBH0700484.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(750..1544)
                     /locus_tag="KR440_003484"
     CDS             complement(750..1544)
                     /locus_tag="KR440_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase"
                     /protein_id="HBH0700485.1"
                     /translation="MEKYATMIKEKLNEGKVLCGVAVSLSDSVVSELIGISGYDFIWI
                     EGEHSALDRKDIQQHMIAAHAGGAASLVRLVNSDPDLVKPIMDMGTDIICFPFINTVE
                     DAQKAVAACTYPPNGIRGYNPQRASYYGNMNYFDYVKNANNETLKMMLIEQKAGYDNL
                     EEIVKVEGIDIIALGPGDLSLDMGFSGYMNRIEIKDMISHAAEICKKYKMPFIIFPTI
                     EKKSIEEWVNIGANMLCFAQDTTFITYVINQLLSFYNEIVPIQKQH"
     gene            1693..2061
                     /locus_tag="KR440_003485"
     CDS             1693..2061
                     /locus_tag="KR440_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0700486.1"
                     /translation="MKYSITDVSKITKISSSAIRFYEEKGLLLPINRKPSGVRYFSEE
                     DVERLIFIKELKNTGLSIKDIHECFHFCDMGDGTIDKRIQLFQQHKQNIKNKIHELES
                     CMDKIDEKIYYLKEKKMCKK"
     gene            2290..>3082
                     /locus_tag="KR440_003486"
     CDS             2290..>3082
                     /locus_tag="KR440_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HBH0700487.1"
                     /translation="MVDFIESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     CQEKHFHLSEEQLLYVIQGEGIQMIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKGI
                     FETKKENKFCDICKNIKPCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIDIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSR"
BASE COUNT         1201 a          363 c          488 g         1030 t
ORIGIN      
        1 aaatataaat gataaatatt atcaataaat attgaatttc aaaatcaatt gtgatattct
       61 atatttagaa aaataaagag atttcagata aagaaatctt ttgtttttta gatgttagtt
      121 agctataact aactattgat taattaaaaa atactacagg aggtaaaaat gagtaaaata
      181 tatattgttt attggagtgg aacaggaaat actgaaaaaa tggcaaattt tgtggctgaa
      241 ggtgtgaagt taaaaggtaa gacaccagaa gttttagatg tgagcttact gaaaccaagt
      301 gatttaaaag aagaagataa atttgcatta ggttgcccat ctatgggagc agagcaacta
      361 gaagaggggt atatggagcc atttgtttca gaattagaat ctatggtatc aggtaaacag
      421 attggattat ttggttcata tggatgggga aattgtgaat ggatgagaga ctgggaagaa
      481 cgtatgcaaa atgctggtgc tacaattatt ggtggagaag gaattacagc aatggaagac
      541 ccaaatgaag aagcaaaaga tgagtgtata gaattaggca aaacgttagc tgaataaatt
      601 tgtatattat aaaaatagta taaatagcaa ctaatgatga tagcagtata taataataag
      661 agagactaat attatgatac ataagaaaat atccttaata gagatagata aaatattagt
      721 ctcttttaat ataaaattag atttataatt taatgttgtt tttgaattgg tacaatctca
      781 ttataaaaag ataataattg attgataaca taagtgataa aggtagtatc ttgtgcaaaa
      841 caaagcatat ttgctccaat attaacccat tcttcaatac ttttcttttc tattgtagga
      901 aaaataataa agggcatctt atattttttg catatttcag cagcatgaga aatcatatct
      961 tttatttcaa ttctattcat atatccggaa aaacccatgt ctaaagataa gtcaccaggg
     1021 cctaatgcta taatatcaat accctcgact tttactattt cttcaagatt atcataacct
     1081 gctttctgtt caattaacat catttttaat gtttcattgt ttgcgttttt gacatagtca
     1141 aaataattca tattcccata ataagaggct ctttgaggat tatatcctct tataccgttt
     1201 ggaggataag tacaagctgc tacggctttt tgtgcatctt ctactgtatt aataaatggg
     1261 aaacaaataa tatctgttcc catatccatt atgggcttaa caaggtcagg gtcactgttt
     1321 actaatcgta ccaaagaagc agcaccacca gcatgggcag ctatcatatg ttgctgaatg
     1381 tctttacggt ctaaagcact atgttctcct tcaatccaaa taaaatcata tccagatata
     1441 ccgattaact cacttacaac tgaatcagac agtgaaacag ctactccaca taagaccttt
     1501 ccttcattta gcttttcttt aatcatagtt gcatattttt ccatttatat catcctttct
     1561 tttgtaagtt ttcttttaca cttcaaagag tacaacttca acctaacttg aagtcaatac
     1621 ttttttgttg actactgtat aaggtatttt tataatattc ttattcatat aaaatttagg
     1681 gaggtagaga ttatgaaata ttctataact gatgtatcaa aaataacaaa aatttcttca
     1741 tctgctattc gattttatga agaaaaaggt ttattacttc ctatcaatag aaaaccaagt
     1801 ggtgttcgct atttctctga agaagatgta gagcgtctaa tatttatcaa agaattaaaa
     1861 aatactggtt tatctattaa agatatccat gaatgctttc atttttgtga tatgggagat
     1921 ggtacaatag ataaaagaat ccaattattt caacaacata agcaaaacat taaaaataaa
     1981 atacatgaac tagaaagttg tatggataaa atagatgaga aaatatatta cttaaaagaa
     2041 aaaaagatgt gtaaaaaata atccttatag aataaagaaa taattttttg atttttctaa
     2101 tataaagagt aaattcaatt ggaaacattt gaggaaagat tttttacact atatttttag
     2161 gaagaattgg acaatgttct atataactat ttatctatac aataaaaaag aaaattatga
     2221 taatgtatat tataaggaga tatttaaagg aataaaaaaa tatttagata aaaactacga
     2281 ggtgagttta tggtggattt tattgagtca aataatgaag tacaaaaatt tgattgggga
     2341 gaggttatgt ggatacacga accatcaaaa acacaattca atagacttag tgctggaatt
     2401 gtaagatttt ttccgggaaa ctgccaagaa aagcattttc atttaagtga agaacagctt
     2461 ttatatgtga ttcagggaga aggaatacaa atgattgatg gtaaaaaggt caatataaag
     2521 gaaacatcta tagtttattg cccaccatat tcagaacatg aaattataaa cactggtaaa
     2581 attgatttag taatattaat tacttatgtt cctcataaat tttcgagttt gagacaaata
     2641 cctatagtat tttctgaaaa caatatacaa gagttagtta atgtaaacat aattgaaaat
     2701 cttgcaaatc aaatatctaa tatattgaag cttagaatat ctgtatatga cttaaagtat
     2761 aaaggaatat ttgagactaa gaaagaaaat aaattttgcg atatttgtaa aaatataaaa
     2821 ccgtgtacta aaaaacttgt taaaagcata aataatgata actttgaaaa agtgtaccat
     2881 tgtgagtatg gtgtttccag tttagagata cctatagtat tagaagaaaa tatagttgga
     2941 tatatagaat gtggtaattt tatagtatat aagtctattg atatagataa aaatttatct
     3001 gaattatcaa gtagttctgg tattgatata aaatacataa acaaaattta caatgaattt
     3061 ccattaaatc ctaaaagtag at
//
LOCUS       DAEPOZ010000318         3053 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.319, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000318 DAEPOZ010000000
VERSION     DAEPOZ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.319"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            168..1460
                     /gene="eno"
                     /locus_tag="KR440_003487"
     CDS             168..1460
                     /gene="eno"
                     /locus_tag="KR440_003487"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBH0700488.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTERLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
     gene            complement(1580..2008)
                     /locus_tag="KR440_003488"
     CDS             complement(1580..2008)
                     /locus_tag="KR440_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBH0700489.1"
                     /translation="MNPVKELNNLWHIVSMQMKTEMCSGKYPTIHGISLVELSILEIV
                     EQYPNSMMKEISEILELPKSTLTSAVKRLELKKFIKKNPCIKDKRRYNLVLTELGLKA
                     QQEHRDIEIIVFRNLLHQLDKDEIETFIRIFSKAVNKSKK"
     gene            2093..2599
                     /locus_tag="KR440_003489"
     CDS             2093..2599
                     /locus_tag="KR440_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0700490.1"
                     /translation="MNLEIIRATSENAIEMGFIHSCSWRKAYKGIIPDKIIDEFTSEK
                     RAEVFQSVIPKAEEEYYLFKVNGIPAGFASLNRSQEDNAPKCVGEIYSIYFHPDFWGT
                     SVTKKGLQFCIDRLSNLGYSYITIWVLKDNTRAINFYKKNGFTCDDFEKEIHIGKKLL
                     EVRYSKKI"
     gene            2717..2941
                     /gene="secG"
                     /locus_tag="KR440_003490"
     CDS             2717..2941
                     /gene="secG"
                     /locus_tag="KR440_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBH0700491.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
BASE COUNT         1152 a          359 c          560 g          982 t
ORIGIN      
        1 ataaaataaa aataggaact tttacaaatt tacccatcaa ataaatttgt acctaatttt
       61 caaaagcaac tttatgtact ataatataga aagtagatta aaatagtaga cggatgttta
      121 cagtaaatga aaatttaact aatagaaaat cgaagggaga tatacaaatg tcagttattg
      181 aattagtata tgctagagaa gtattagact caagaggaaa tccaactgta gaagttgaag
      241 tagtattaga agatggagca atgggaagag caatagttcc atcaggagca tctacaggag
      301 cttttgaagc tgttgaatta agagatggag ataagggaag atacttagga aaaggtgtag
      361 aaacagctgt tgctaacgta aatgaaataa tagcacctga aatagaagga atggatgcat
      421 ttgaccaacc agctatagat gcaatcatga tagaattaga tggaactcca aacaaaggta
      481 aattaggagc taatgctata ttaggtgttt caatggcagt agctagagca gctgctgatg
      541 aaataggttt accattattc caatatttag gtggagtaaa tgctaaacaa ttacctgttc
      601 caatgatgaa catattaaac ggtggagagc atgctgataa taacgttgat gttcaagagt
      661 tcatgatatt accagtagga gcttgttgct ttaaagaagg tttaagaatg ggagcagaag
      721 tattccattc attaaagaaa gttttaggag aaaaaggatt agcttgtggt gttggtgacg
      781 aaggtggatt tgctccaaac ttaggttcaa acagagaagc tttagaatta atagttgaag
      841 ctataacaaa agctggatat aagccaggtg aagatgttat gttaggactt gacgttgctg
      901 ctacagaaat gtacaacaaa gaaactaaaa aatacgtttt agcaggagaa ggaaaagagt
      961 taactgctgc tgaaatggtt gctttatatg aagattggtc aaacaacttc ccaataataa
     1021 ctatagaaga tggtttagac gaagaagatt gggacggatg gaagttatta actgaaagat
     1081 taggaaataa attacaatta gttggtgacg acttatttgt tactaacaca gagagattag
     1141 aaaaaggaat tgaaaatgga gtagctaact ctatattagt taaagttaat caaataggta
     1201 caataactga aactttagat gctatagaaa tggctaagag agcaggatat actgctgtta
     1261 tatctcacag atcaggagaa actgaagatt ctactatagc agatttagct gttgcagtaa
     1321 atgctggtca aataaaaact ggtgctccat caagaactga tagagttgct aagtacaacc
     1381 aattattaag aatagaagaa atggttggag aacaagctag atactgtgga ttaaaatctt
     1441 tctataattt aaaaaaatag ttattgaaga ttaaaaatat ggcattaaag attaaaaaga
     1501 taacataaaa gataaaaatt tatcttataa gggataagtg atagttgatt tttcaattca
     1561 cttatccttt ttttatattt catttcttac ttttattcac agctttagaa aaaatacgaa
     1621 taaaagtttc tatttcatct ttatctaatt gatgtaagag atttctaaat acaattattt
     1681 ctatgtctct atgttcttgt tgagccttta aacctaattc agttaagact aagttatacc
     1741 tccttttgtc tttaatacag ggattctttt taataaattt ttttaattca agacgtttta
     1801 cagcacttgt taatgtgctt tttggcaatt ctaaaatttc actaatttct ttcatcatac
     1861 tattgggata ctgttcaact atttctaaaa tacttaattc tactaaagaa ataccatgaa
     1921 tagtaggata cttaccacta cacatttctg ttttcatctg catactaaca atatgccaca
     1981 ggttgtttag ctcttttaca ggattcattg acttttacct ccatatatga tacaattatc
     2041 gcactaatat agtacgataa tcgtattaat ttgtcaatag gatggtgttt ttatgaattt
     2101 agagattata cgagctacaa gtgaaaatgc tatagaaatg ggttttatac attcttgttc
     2161 gtggagaaaa gcgtataaag gaattattcc agataagata atagatgaat tcacttctga
     2221 gaaaagagca gaagtttttc aaagtgttat tccaaaagct gaagaggaat attatctttt
     2281 taaagtgaat ggtatacctg ctggttttgc ttcattaaat agaagtcaag aggataatgc
     2341 tccaaaatgt gttggagaaa tatattctat atatttccat cccgattttt ggggaacatc
     2401 tgtgacaaaa aaaggattac aattttgcat tgatagatta agcaatttag gatattcata
     2461 tataacgata tgggttctaa aagacaatac tagagctata aatttctata aaaaaaatgg
     2521 ttttacttgt gatgattttg agaaagagat acatattgga aaaaaattat tagaagtacg
     2581 ttattcaaaa aagatatagt aattgcatat tatttttgtt tgttatttat acttcaatag
     2641 tcttgaaatg actatattat tgtgatacac taaagagtaa tgaaatttaa gtaataaata
     2701 gataggaggg gcaataatga gcaacatttt aatgggtgtg caagtagtgc tagggatttt
     2761 actagtaatt agcataatgc ctcaagattc aaaaaatgct ttaccttcag aatttggtgg
     2821 agaaggaagt caagcttact ttaaaccaaa aggaaaacaa gcatttcttg ctagagttac
     2881 aaagataaca gcagtattat tttttataaa tgcattggca ttacttatag ttaagaagta
     2941 gtgatattgt tgaataactg gccattattt aattagtgca tttgctagat atttaaaaat
     3001 taaatttagt ttttaataaa agtacatcaa cataaaaagt acgtaaaata aaa
//
LOCUS       DAEPOZ010000319         3017 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.320, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000319 DAEPOZ010000000
VERSION     DAEPOZ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.320"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            71..751
                     /gene="pgmB"
                     /locus_tag="KR440_003491"
     CDS             71..751
                     /gene="pgmB"
                     /locus_tag="KR440_003491"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBH0700492.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            748..>3017
                     /locus_tag="KR440_003492"
     CDS             748..>3017
                     /locus_tag="KR440_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_097546124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBH0700493.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFYRELDMKNGILNRRFIVNINDKKFEVRVTRFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAVSHESDYNYCENSITMDVLKNKVASIEKYVSITSTRDYKESELLR
                     KCEDILNIELSKSYDDILNEHTKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNCIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLPYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGK
                     PIKIIVYGKKYILEKY"
BASE COUNT         1238 a          275 c          566 g          938 t
ORIGIN      
        1 atcttaaata caagatagaa aataaattga ttattagtct aaaaatttct tgtagaaaga
       61 ggaaataaga atgattgaag catttatatt tgatttagat ggtgtaatta cagatactgc
      121 atattatcac tatatggctt ggagaaaact agcccataaa gttgggatag atatagacac
      181 aaaatttaat gaatctttaa aaggcataag tagaatggag tcattggaca ggatattaga
      241 atttggaaat aagaaatatt cattttcaga agaagaaaaa gtgagaatgg ctgaagaaaa
      301 aaataattac tatgtatcat tgatagatga gataacttca aatgatattt tgccaggcat
      361 agaaagcttg ttaattgatg taaaatctaa taatataaag ataggtctat cttctgcaag
      421 taagaatgca ataaatgtat taaatcattt gggtataagt gacaaatttg attttatagc
      481 tgatgcagga aaatgtaaaa acaataaacc acatccagag atatttctta tgtcagcgaa
      541 agggcttaat gtaaatccac aaaattgtat agggatagag gatgcaagtg caggtataga
      601 cgctataaac tcagctaata tgttttctgt tggtgttgga aattatgaaa acttaaaaaa
      661 agcaaattta gttgtagatt caacaaatca attaaagttt gaatatatac aagaaaagta
      721 taatgagtat atagttagga gaattatatg aaatgtttat ttagtaatgt agatgagtgg
      781 aagattatac aagataagat tgaaatcaaa gaaaatagat tagcagagtc cataatgagt
      841 ataggcaatg gatatatggg tatgagagga aactatgagg aaaattatag tggagataca
      901 cacaaggggt cttatatagc tggagtatgg tttccagata aaactcgtgt tggttggtgg
      961 aaaaatgggt atccagaata ctttggtaaa attcctaatt cagtcaacta tataggtgta
     1021 agaattttca tagatgatgt agaattagat ttagcaaaat gtaaagtaga agatttttat
     1081 agagaattag atatgaaaaa tggtatttta aatcgtaggt ttattgtaaa tataaatgat
     1141 aaaaaatttg aagttagggt tactaggttt ttaagtatat ctgttaagga acttgcagta
     1201 attaaatata gtataaaatc attaaattct aatactaaaa taaaatttgt accatattta
     1261 gattcaaata taaaaaatga agattcaaac tatgaggaaa agttttggaa tggaatttct
     1321 tcaagttctt tagaatataa aggtagtatt ctttctcgaa caaaggagaa tgcatttaat
     1381 acaccagtat tcactgtggg ttcaatgatg tatattaaga caaatggtga tgtagaagct
     1441 gtatctcatg agagtgatta taattactgt gaaaattcga taacaatgga tgttttaaaa
     1501 aataaggttg caagtataga aaaatatgta tctataactt caactagaga ttataaggaa
     1561 agtgagctat taagaaaatg tgaagacatt ttaaatatag aactatctaa aagttatgat
     1621 gatatattaa atgaacatac aaagttatgg aataaaagat gggaaagtgc agatgtaaaa
     1681 ataaatggag ataaaagtag ccaacaaggt ataagatata atttattcca actattttca
     1741 acatattatg gagaagattc gcgattaaat ataggtccaa aaggatttac tggtgaaaag
     1801 tatggaggag ctacttattg ggatacagaa gcattttgtg ttccagtata tctaggtgta
     1861 gcagataagg aggtaactag aaatctttta gaatataggt acaatcagtt agaacaggca
     1921 aaggataatg caaaaaaact agggcttaaa ggtgctttat atccaatggt aacatttgat
     1981 ggagtagaat gtcataatga atgggagata acatttgagg aaatccaccg aaatggaagt
     2041 atagtatatg caatatataa ctatacaaat tatacaggtg actataaata tataaaagaa
     2101 aaaggaatag atgtaataat agaaatagct agattttggg caagtagagt tcatttgtct
     2161 acaaaaaaag atttgtatat gatacatgga gtaacaggtc caaacgagta tgaaaacaat
     2221 atcaataata attggtatac taattgtata gctaaatggt gtttagatta tgctgtagaa
     2281 aatatcttac gattagagaa agagtgtaat gaatgtattg acagaaataa tgtgactagg
     2341 gaagaagttg agttttggaa aataatatca aataaaatgt atctgccgta tgatgaggaa
     2401 ctacaaataa taattcaaaa tgataatttt ttagataaag aatttgtaga agtaaaggat
     2461 ttaccaaaag aaaattttcc tcttaatcaa aattggtctt gggataagat tcttaggtcg
     2521 tgttttataa aacaagcaga tgtattacag ggaatttact attttggaaa tagatttact
     2581 aaagaagaaa aaaagagaaa ttttgacttc tatgaaaaat atactgtcca tgaatcttct
     2641 ttatcttcta gtatttattc tattattgcc tcagaaatag ataacctaga aaaggcttat
     2701 gaattatact caagaacagc taggcttgat ttagataatt acaataatga tacaaatgat
     2761 ggactgcaca taacttcaat gtctggttca tggctttcca ttgtacatgg atttgcaggc
     2821 atgagaacat ggaatgaaac tctatctttt gaccctaagt tgccaaaaga gtggaaaaat
     2881 tattctttca atataaatta tagagataat attattaatg tagatgtatg tagtgattta
     2941 attaaaatag aaaatttaaa aggtaaacca ataaaaatta ttgtatatgg gaaaaaatat
     3001 atattagaaa aatatat
//
LOCUS       DAEPOZ010000320         2980 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.321, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000320 DAEPOZ010000000
VERSION     DAEPOZ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.321"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..632
                     /locus_tag="KR440_003493"
     CDS             <1..632
                     /locus_tag="KR440_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700494.1"
                     /translation="KQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKE
                     NNKYTSKEKNSEDIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLS
                     SIKKDVTNARNKCKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESA
                     LKLINTKSPKYIGKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            669..1826
                     /locus_tag="KR440_003494"
     CDS             669..1826
                     /locus_tag="KR440_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBH0700495.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRHFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            1978..2538
                     /locus_tag="KR440_003495"
     CDS             1978..2538
                     /locus_tag="KR440_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBH0700496.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            2566..>2980
                     /locus_tag="KR440_003496"
     CDS             2566..>2980
                     /locus_tag="KR440_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700497.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDI"
BASE COUNT         1293 a          251 c          537 g          899 t
ORIGIN      
        1 acaaacaaga tagaaatata caaaataaag aaagtaatga agaaggaagt aatgatataa
       61 aagaaaagaa taattctgat agtaatgttt cagtgaataa taaatcagaa aaacaagaag
      121 ataatacaaa agaaaataat aaatatacaa gtaaagaaaa aaatagtgaa gatataaaaa
      181 aaatagatat ggaaagtata ggaattttta agaaagaaat ttataatata gaaagagcag
      241 ctttagttcc aatgagagaa tgtatacctt acactaagga tatagcaaaa aatataacta
      301 aattatctag tataaaaaaa gatgttacaa atgccagaaa caagtgtaaa gaagttgtaa
      361 aagaatatga aaatatgcag attccagaac tttctaagga gaagtataca gaaacattga
      421 gtaatgctag agatgacgta aagaaagcat atgagcttag agaaaaatct atggaaagtg
      481 ctttgaaatt aataaatact aaaagcccta aatatatagg gaagataaca gaatatcttg
      541 atttatcaga taagcagata gaaagtttta aaaatagatt gaacgattta aaagaagaaa
      601 taaataattc agaaacaaat aatattaatt aataagattt aaagatataa gttaaaaggg
      661 agattatgat gaataaacag agtataggaa aactaaatat gttttttatg gggattactc
      721 aaagaaacct tgataatagg cattttttta ttgagttaga ggtgattttt aaatctggaa
      781 gaaaagagtt taaaggaatt gcaatagagg aagataataa atataaattt aattttaaag
      841 gaaaatcaga tttatatacg tttaatgagt tgcttaagaa tatttctaaa gaggcagaaa
      901 attatgatgg attagtattt aaatatgttg agcgaggaac tattgttgta attgaaggag
      961 acaataagaa ggtaaatgta aaatatttag ataataaaga agaagtacct aaaatagatg
     1021 aatttacggc atctcaaata aaaaacagag attattatgt aaaagtagga caagcaaatg
     1081 cacttttaaa agaaattgga gtacttacaa aagatggtaa gataaaaaat gataaaataa
     1141 gaaaatacaa tcagatagac cattttgttg aattgataga tagtatttta aaagaaataa
     1201 aggataaaga ctgtataact atcttggatt gtgcttgtgg aaaatcttac ttaagctttg
     1261 tcttaaattt ttatataaaa gaagtattga agaaaaactg ttattttata ggaatagatt
     1321 attcagatgt agttatagaa gcatcaaaaa atatggctaa aaacttgggt tataaaaata
     1381 tgtcatttat aaaagaggat ttgactaatt atacacctaa tagggatgtt gaccttgtaa
     1441 taagtctaca tgcttgtgat acagctacag atatggcaat aggtctagga attagagcaa
     1501 aatctgaagc tatagtagta gtaccatgtt gtcataagga gttattaggg caatatagat
     1561 atgaagctat ggaaccaata ttaaaacatg gtgtatttaa agctagattt gcagatttga
     1621 taactgatgg tttaagaaca ttgttattgg agggcaatgg atatgatact tctgtagtag
     1681 aatacatatc tccacttgat acaccaaaaa atttaatgat aagagcgata aaaactaaaa
     1741 cgaataatga caaagcatta aaagaatata aagaactgaa atcacaattt ggagtggaac
     1801 caaccttaga aaaacttata tattagttaa aaatgtaaaa tttacgaaaa gcttgcttaa
     1861 atatgcaagc tttttggtat tatataggta tagtcatatc cagagttttg atttataatt
     1921 aaagctgtgc tataatattt gattaatcaa atcaataaat gttacatgga ggtaaaaatg
     1981 aacaaattga atatatgtat tgatatagat ggaacaataa caagccctta tcattttctt
     2041 ccatacctaa atgatatctt taataaaaat ataactgaag cagagtgtat aacacataat
     2101 tgggaagaat tgtatggttc gggaataaga gatgtgtatg cggagtttaa taataagtat
     2161 atacactcat atgatgaagc aaagattgta gagggtgcta cagaggtaat tagcacatta
     2221 tctaaaggac ataatttatc ttttgtaacg gctagacatg agtgtttaac tgatgtaact
     2281 aaaaattggc tatcgagaca aggattttca gatatagatg tttacttatt aggaagtgat
     2341 tataaagttg aaaaggcaag agaactgagt tgtgacatat ttatagaaga taatccttta
     2401 aactcagtgc aactagctga tgatggtgtt aaagtaatac ttctggatac taactataat
     2461 aaagatgtta aacatcaaaa tatagttaga gttagtaatt ggatagatat tgaaaagatt
     2521 ataaaacaag gtatttaaag atttgtagat tgaaaggggg agcatatgat agatttaaaa
     2581 attgaaaata gaaaaggaaa aatccatgta aagagcagtg aaataaaaga cattttagaa
     2641 ttacgtccag acttcgaata tgtacaagat atatcaaata ctataaatca agaaaatata
     2701 ttggtatttg attgccagtt gacaggaaat tcttttgata tggatgattt agatatagaa
     2761 gaaatattgg aagagttagg tgaagaaata gatgagtctt atttcagtgt tttatttgaa
     2821 gatgtaagag cgtatttaaa agatgctact gatgagatag aagcagactt acaagataat
     2881 tacttatttg ataatttgag atgttatttt gatatataca atataaatca agaatttaca
     2941 gattttaagt ttgtattttt agtttctttt aaagatataa
//
LOCUS       DAEPOZ010000321         2924 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.322, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000321 DAEPOZ010000000
VERSION     DAEPOZ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.322"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            458..760
                     /locus_tag="KR440_003497"
     CDS             458..760
                     /locus_tag="KR440_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700498.1"
                     /translation="MKYSKGFKLIYSLVEYSKKFKVIYSVMKYSKGFKLIYSLVEYSK
                     RFKLIYDVMKYSKEFKLIYSLVEYSKRFKVIYSVMKYSKRFKAIYNAVKYIQGDLK"
     gene            972..1241
                     /locus_tag="KR440_003498"
     CDS             972..1241
                     /locus_tag="KR440_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBH0700499.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIGQCRKWLEETVSN"
     gene            complement(1458..1703)
                     /locus_tag="KR440_003499"
     CDS             complement(1458..1703)
                     /locus_tag="KR440_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HBH0700500.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            2240..2800
                     /locus_tag="KR440_003500"
     CDS             2240..2800
                     /locus_tag="KR440_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin regulator"
                     /protein_id="HBH0700501.1"
                     /translation="MFFGNISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAE
                     PWFKMKDDEKKAIEAENQRKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYK
                     YKKVKFEGKVIQVIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGI
                     SAGTITYESTMGGKITIPGIAVEKIN"
BASE COUNT         1085 a          304 c          427 g         1108 t
ORIGIN      
        1 ccttttattt aaaaatatat atcttttatt tgaattttat atgttttatc tactcactat
       61 ataatagata tactcctaaa ataaaaaaat atataataat gttattattt aaaattatta
      121 tgtgttatat taaaacttaa tgcaaaaaca atttttatat tatataatga aacttaatta
      181 aatatagcta aaaaatcatt caatacatta aataaatatt taactttaat aatctatatt
      241 taattatggc tataacatag aattatcaaa tatattgaat gaatatttga ctcttggaat
      301 gtgtaattaa attaagtttg gttattcgat ggaaggcaaa tttgatataa aaactccatt
      361 taaagtgcca gataaaaact ttatggttat ataaaagtta aaagtaattt atagtgttat
      421 agaatattca aagggattta aagtaatcta tggtgttatg aaatattcaa agggatttaa
      481 actaatctat agtcttgtag aatattcaaa gaaatttaaa gtaatctata gtgttatgaa
      541 atattcaaag ggatttaaac taatctatag tcttgtagaa tattcaaaga gatttaaact
      601 aatttatgat gttatgaaat attcaaagga atttaaacta atctatagtc ttgtagaata
      661 ttcaaagaga tttaaagtaa tctatagtgt tatgaaatat tcaaagagat ttaaagcaat
      721 ctataatgct gtgaaatata ttcaagggga tttaaagtaa tctatagtgt tatggaatat
      781 tcaaagggat ttgaagtaat ttattgtgtt ttgaaatatt caaaaatatt tttggaatag
      841 tttttataaa tttaccagta ttaataattt tatgtttaac atttagatga cttaaaatca
      901 actatttgca cttttgtagt catctaaaat cattttataa tatttttaaa taatggaaag
      961 gatgtttgaa catgtttgaa tcaagatgtg gtgtctgttg tgacagttgt acaaaaaaag
     1021 aacaagtaaa ttgcactggt tgcccaacta tggaaaaacc attttggggt ggagaatgta
     1081 aggttaaaac ttgctgtgaa agtaaggaac ttaatcattg tggtgaatgt gatactttcc
     1141 cttgtgatat gcttttaaat aagggaaaag accaaggctt tgaccctatg gtaaatattg
     1201 ggcaatgtag aaaatggctt gaagaaacag tttctaatta attgttttag aatgctttgt
     1261 taagtaattt atttaatcct ttgttaggta gtatgtttag tcatttattg attggtatat
     1321 ttatacattt attgatgggt atatttagcc atacaaatag atttacataa aatttacgac
     1381 taaaaaagag ttgagtatta gtttttatta tttaaacgga tattcaactc ttttttatat
     1441 ctaacttttt tgatttttta atctttccaa taccaccact ttaatttttc tggttctgga
     1501 ttttcagttg atgcaaaata tactgcacat ctgtatacat ataactgaca tctgtctatg
     1561 tgctgtccac gcattaaaca ttccttttca tacatttgtg ctgggtttgc atctttaagt
     1621 gatttaatag ttgtatatcc taacatgatt aaagattttt ctgttgcttt ccctacccct
     1681 ggaatttttt gtagttctcc catacttcac cttctttctg atatattatt tttgtattat
     1741 acttagtacc agatattttt ttattatagt taatatttaa ttttttatta tatcacttta
     1801 tttatgctct ttcatctatc tatattttac cacctctaaa gtactgaatc atttaattac
     1861 atcataatat agttttatac aaataaaata ctttatgttt catttaatat ataaaattca
     1921 ccttcaagaa aattatatta taatctgaca tttttacctc atttttcaaa atatattgaa
     1981 tcttcttgat ttattttgta aaattatgct taggggaaat atattttagg aaaatatgaa
     2041 tatataattt ttagtcaact agttatttta agtttttaaa ttttaaaata aaatatatct
     2101 aataaaaggg agattgtatt atgttttcta aaaaaaatga gggtaacgaa tttagtaatg
     2161 aaagaaaagg aagctctaag aaaataatta aattctttaa gagcacaaag gatattgctc
     2221 tactggcatt tattttggtg tgttttttgg caatatatcc tcaccagctt gttctgaaga
     2281 ccatgaggag gtcatttcta atcaaacatc agttatagat tctcaaaaaa cagaaataga
     2341 aactttaaat agcaaattgt ctgatgctga accatggttc aaaatgaaag acgacgaaaa
     2401 gaaagctatt gaagctgaaa atcaacgtaa agctgaagaa gctaaaaagg ctgaagaaca
     2461 acgtaaaaaa gaagaagaag agaagaaagg atatgatact ggtattactt atgaccaatt
     2521 agctagaaca cctgatgatt ataagtacaa aaaggtaaaa tttgaaggta aggttattca
     2581 agttattgaa gatggtgatg aggtgcaaat aagattagct gtgtctggaa attatgataa
     2641 ggtcgtacta tgtagttata aaaaatcaat aactccttca agagtgttag aggatgatta
     2701 cataactata agaggtataa gtgctggaac tataacttat gaatcaacta tgggtggaaa
     2761 aataaccata ccagggatag ctgtagagaa aattaattaa tattgttttg tattatagtt
     2821 aatattttat attatagtca atatgtttaa agatgttttt ataattgcaa ataaacagtt
     2881 acaaggctct aaattagttt ttgcttttag catattatct attt
//
LOCUS       DAEPOZ010000322         2915 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.323, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000322 DAEPOZ010000000
VERSION     DAEPOZ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.323"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            326..2137
                     /locus_tag="KR440_003501"
     CDS             326..2137
                     /locus_tag="KR440_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBH0700502.1"
                     /translation="MKKKYDFLIYSAIWLVIIIFFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILYNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVAAALSKTFNIKETCARIGSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSIMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVKKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(2225..2806)
                     /locus_tag="KR440_003502"
     CDS             complement(2225..2806)
                     /locus_tag="KR440_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700503.1"
                     /translation="MLYLLKINQFERSYDIGIFKNEQSIYKFIEKIPFVKKDNSNFNY
                     TNYYMEFEDIPEYYEVNFNNYLYIITRFSFIPDGTEIFFTWEEIHYWDEKVATEKTFI
                     NGETLVDSYNFPNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALGSEDGEYVELTN
                     DSILYLLDIEAVEIREQSKNIEEFLYKYKKRFK"
BASE COUNT         1089 a          301 c          410 g         1115 t
ORIGIN      
        1 tgtatatgcc aaatttaaaa agagacataa agacattata caaatatatt attggggtga
       61 tattttataa gaaaagaagt gatgcagtaa attaattgag caatttaaaa atgatttaat
      121 gatagtgttg atgaatgttt aaaaagaaat tttagaaaaa cgtatattgc tgtttgtgtt
      181 ggaggtgatg aatttgtata atttataggg tataaatgat taaactactt aagataaata
      241 catatcactg taaaatattg ataatcattt tttataagag attgattatt tgcaaattat
      301 atatattaca aatgaggtaa caataatgaa gaaaaaatat gattttttaa tttattctgc
      361 tatttggcta gttattatta ttttttttat attttctcta tatcatagta tagagcatat
      421 taaaattata agtcacactg gtactattcg cagtgaaact caaaaggttg ttaagcaaga
      481 attgaataat gaaagaaatg atgacttgat aaaacgactg gataatattc tgataaagct
      541 aagaacaggt aatggagaaa atggatttca aagatgtgat aataaagaat ttcaacaaaa
      601 actaaatcaa atggattcta tgtgggaatc tatgaagaaa gaaattataa aagttagaaa
      661 tggtgcttca ggtgataaat tatataaatt gagtgaagag tattctgtgt tgtctaatca
      721 aattgttttt atatctgaaa aacactctaa tgcaaaattg tattcttttg ctactgcact
      781 atttatttat cttatattct caacaataag tcttttaatt tgggaatatt ataataaaaa
      841 aaggtttaaa agaatatttt atactgataa tttaacaaaa ataaaaaatc aggttgcatt
      901 tgaaaacaga gcgattgaaa ttttgtacaa tgcatctaac aaagagtatg tactgctaaa
      961 tattgatatt gacaatttta aatatattaa tgatacacat ggatatgaat atggagataa
     1021 agttctaata atagttgcag ctgcactttc taaaactttt aatatcaaag aaacttgtgc
     1081 aagaattggt tcagataatt ttgtaatact tgcaaaatat agagattctc ttttagagga
     1141 tattcgggaa atgctaacta atgctataat ctctgaatta gatatgaatg taactcaaac
     1201 tatatcttat tgtataggag cgtacttagt tgaaattgat aatttaggtt ataaatccat
     1261 caattctata atggataagg caaatattgc acacaaggta agtaaaacaa gaggaatttc
     1321 ctccacagta tggtataatg aaaatctgtt aaaacagtta caaatggaaa atagtattta
     1381 taattatatg tataaagcga tagaaaatga agaatttcat atgtatttgc aacctaaatt
     1441 tcaaatatct tctttaaatg ttgtaagcgc agaagctctt gtaagatggt tttctccaga
     1501 acttggtttt ttatcaccag atgaatttat tccattgttt gaaaaaagtg gatttattat
     1561 tgaattggac ttttatatgt taaaaaaagc gtgttcattt gtaaaaaaaa cgtttatgaa
     1621 aaaaaatcaa tatacatatc cgattgcagt taatttttca agggttacta tttaccaaaa
     1681 tagcttttat caaaggtttt tagatactgt taaagaatat gaaattcctt ttaagtatat
     1741 agagatagag gtaactgaaa gtgcttttaa tgaaatatct caaccagtta tttcgatttt
     1801 agaggagctt aaaaagttag gatttttaat ttcgatggat gattttggat caggatactc
     1861 ttcattaagt cttttatgtt ctctatctat taatggattg aagttagata aaagcttact
     1921 taaagaaaca tttaaccggg agaaagtata tagtattatt cagtgtatta ttgaaatgtc
     1981 acatcgtata ggcatgtctg ttgtatgtga aggtattgaa actaaaaaag acttggaatt
     2041 tttaaataca gttaaatgtg atgtaggaca agggttttat ttctctaaac cgatagaaga
     2101 aaaagaattt tttaacaaat atgtaactaa aaaataatat aattattttt atcagtctat
     2161 acattgagtc attttatatt tcgttaaatg actcaatgta ttgttatgta ttttaaaata
     2221 taaattattt aaaacgtttt ttgtacttat atagaaattc ttctatattt tttgattgtt
     2281 ctcttatttc aactgcttct atatctagta aataaagtat agaatcattt gtaagttcaa
     2341 catattcacc atcttctgaa ccgagtgcat gtctctcaat ttttaatcct ttttcttcat
     2401 aatatttttt tgtttctata tataatgatt ctcgcttttg tatgtagtct ttcacttcat
     2461 tatttggaaa gttatatgaa tccactaatg tttcgccatt tataaaagtt ttctcggtag
     2521 caactttttc atcccaataa tggatttctt cccaagtaaa aaatatctca gttccatctg
     2581 gaatgaatga aaacctggta ataatatata aataattatt gaaatttact tcataatatt
     2641 caggaatatc ttcaaattcc atatagtaat ttgtataatt aaaattacta ttatcttttt
     2701 ttacaaaggg tattttttca ataaatttat atattgattg ttcattttta aaaattccta
     2761 tatcatagct tctttcaaat tgattaattt ttaataaata caacattctt tctcctttta
     2821 ttcaaaatat aatttttata aatttactaa tttttagctt ttttaattat atatttacat
     2881 atgtttatat gatactataa tgatgtgttt taaat
//
LOCUS       DAEPOZ010000323         2896 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.324, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000323 DAEPOZ010000000
VERSION     DAEPOZ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.324"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            169..873
                     /locus_tag="KR440_003503"
     CDS             169..873
                     /locus_tag="KR440_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0700504.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILDTNI
                     LIETVWGFGFRLKEKS"
     gene            875..2362
                     /locus_tag="KR440_003504"
     CDS             875..2362
                     /locus_tag="KR440_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBH0700505.1"
                     /translation="MNNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDENTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVFSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
BASE COUNT         1171 a          260 c          500 g          965 t
ORIGIN      
        1 aaaattgcct taaaatccaa acaattttaa aactaatgta ctataaaaag ctggtataat
       61 ttaaatatag taaaaaataa gatttatctt atctattggg atttaattat ttaatcaata
      121 tatttatacc atttaattta aataaaaagt ctattaagga gtgagcttat gagtaagtta
      181 atttatatag cagatgatga agataatata agaaatttgg taaagacatt cttaaaaaat
      241 gaaggtcatg atgttatgga ttttaaaacg ggtgatgaac ttttagaaca atttaatata
      301 aaggagtgtg acttagtaat actagatata atgatgcctg gctcaagtgg ttttgaagta
      361 tgtacaaaac ttcgtgagaa aagtacagtc ccaataatca tgcttactgc aagagataca
      421 gatatagatt atattacagg gataacctta ggtagtgatg attattttac aaaaccattt
      481 agcccaatgt ctttggttat gagagtaaaa tctatattta gaaggataga gtttgaaaag
      541 aaacaaaatt atgataaata tagtaactcg atagatatgg aattaaaatt tggtgatgta
      601 ataataaata aaaagaataa aatcgttaca agtaaaaatg taaatataga ccttacacca
      661 aatgaatata atctattgac ttatttgttt gaaaatatag atagagcagt ttccagagat
      721 gaattattaa acaaaatatg gggatacgat atagaagttg agacaagggc tgctgatgat
      781 acagtgaaga gattacgtaa aaaaatttta gatacaaata ttttaataga aactgtatgg
      841 gggtttggat ttaggttgaa ggagaaaagt taagatgaat aatggaaatg acaagaatag
      901 aattggaaag attaaatcca aatttaaatt aaagttctca aagaaaaata taagcataaa
      961 aactaggatt ttgttgagca tactttgttt gataattata gtatttatga taatattagt
     1021 atcttttaat gtatttcttg atacatatat aaaaacaact gcgaatgaag aattgacgaa
     1081 gtctacagaa atagtagaac atatggatag taattttaga cctattaagc caccacaaga
     1141 tgatggaaag cttcctccca aagtattatc taattttatg agaaatgtac aagataaagt
     1201 taggatggca gaaacacaat cagatgctga tgctatggta gttgattcta agtataacct
     1261 gatatttcca acaagagagg atgatttctt aaaaaatgtt gatgagatgg agctgattag
     1321 ggagtgtatt caaaatgaaa aattagattt aaattcagat gaaaatacac ggatatctac
     1381 cagaaataag gattattata tttcaactgt aaaaattact agtatatatg gtgaacaaga
     1441 caaatatctt atacttttta tagacatatc aaaaactcta aatctagcac aaaagataaa
     1501 tattgtatta atatctgtaa tgtgttttgc tggaatatta gcaatattta cagctgtgat
     1561 tttatctgag aaaattgcta aaccgataaa agagttgtgt gagtttgcaa aaaaaatggg
     1621 tcaaggagat ttcaaaagaa cctactttga cttttcagat aaagaattag tagagctctc
     1681 aactgttatg aataagagtg ctgagtatct ggataagtat gataatgaac aaaaaatatt
     1741 ttttcaaaat gcatctcatg aattaaggac accactcatg tctattaaag gttatgcaga
     1801 agctataaaa tataatgtta tagatagtaa acatgcaagt gatataattc ttgaagaaag
     1861 tgataggtta agtgatatgg ttgaagattt attatacatt tctaaaatag ataatataac
     1921 taaagatttt gaacttgtag aatgtgattt aagagaagta ttttcaaatt gtggagctag
     1981 gcaaaatgta agagctataa ataaggggat aaaatttatt tatgaatttg atgaagaaat
     2041 ggttttattt gaatgtgatg aaaaaaacat ttccaaagca tttatgaatc ttatagaaaa
     2101 tgctttgaga tacgctaaga gtgaaataaa aatagtgtgt aagtataatc aaaaaaatat
     2161 agttgtgata atagaagatg atggaattgg aatcaagaaa gaagatttac ctcatgtttt
     2221 tgaaagattc tataagggag tagggggaaa tcatggaatt ggattatcaa tagttaaatc
     2281 tattgtaaat aagcatggtg ggcgtatata cgtagaaaat ggcaaaaaag gagctaagtt
     2341 tactatatta tttaaattgt aaaaaaatta gagaaaatat aaagatgttt gaagttgttt
     2401 tttatatata tctgttgtat actgttgaaa aaagaacaat attaagctga catatattac
     2461 aataattgac atttaaagta aatgcttaaa tatagatacg aaaggaaaag taaaatctga
     2521 agtgtattta ggtttaagta agcttcttat tttataaaaa atatgtatga aagaacagct
     2581 gatttttaat attacaaatt tagtaacggt tgtatttgaa gcttttgtca tccatatgtt
     2641 tttatcagac tttttaggtc agaaagaagg gtatataaaa gttgtaagat atgctaaact
     2701 gggttttata atatgtttag gattctgtaa tttaataact ttaaactcta aaatcacaat
     2761 gcctttaatt tttatcctta ttttttcaac atcattttta tataaaggaa atttaaagac
     2821 acgattgttt actactgttt tattaagtat attctttata ctgtcagaaa ttgtagttac
     2881 aagtattttt gtttta
//
LOCUS       DAEPOZ010000324         2895 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.325, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000324 DAEPOZ010000000
VERSION     DAEPOZ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.325"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            58..963
                     /locus_tag="KR440_003505"
     CDS             58..963
                     /locus_tag="KR440_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HBH0700506.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            972..2156
                     /locus_tag="KR440_003506"
     CDS             972..2156
                     /locus_tag="KR440_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0700507.1"
                     /translation="MEIRYAKEEEIESIKEIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     RIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIEKDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNTYINE
                     YI"
     gene            2271..>2895
                     /locus_tag="KR440_003507"
     CDS             2271..>2895
                     /locus_tag="KR440_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HBH0700508.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLL"
BASE COUNT         1203 a          239 c          486 g          967 t
ORIGIN      
        1 ttatatatac aaaataaaat ttttaaaatt aagcaataaa aaaggtagga ggattttatg
       61 tttttcaaag atattgaact aaattcaaaa aaagagttag acccatattt tgatttagta
      121 gattatgaag cttgtgagta ctgttttagt actttatata tgtggcaaca tgtttataag
      181 acaggatatt atataggtga agattttgcc gttttagttg gggaatacga aggagatagt
      241 ttctcaatat taccacttgc taaaaaagat aaacttccag aagttgtaga ttttgtcctt
      301 gaatattttt ctaaaaataa taaaaaaata tacttaagag gtattactac tgaagtagta
      361 gagtttttaa aagaaaaata tccaggtaga tttgagtata tagaagaaag agatttattt
      421 gactatattt atgatgcaga aagtttaaga actctagctg gtaaaaagaa tcaaaagaag
      481 agaaaccata taaactattt cttaaaagag tatgcaggta gatatgaagc taaattatta
      541 gataaagaaa actttgacga gtgtttagta ctaatgaagg aatgggaaag taataaggaa
      601 gaaaataatg aatttgatga aagtatggat gacgaattaa taggtatcaa aaagatattt
      661 aaccattatg acatattaaa agataaggta aaagtgtttg gtgtttatgt ggatggaaaa
      721 ttagaggctt ttagtatagg agaactttta aatccaaata tggcattgat tcatatagaa
      781 aaagctaatc ccgatataag aggtctgtat ccatttataa atcaacaatt tttggtaagt
      841 gaatttaagg acgttgagtt tgtaaataga gaagaagatt taggtattga aggattaaga
      901 aaagctaaac tttcttatca tccttgcaga tttgttgaaa agtacagtgt tagggaggct
      961 taatagacta tatggagatt aggtatgcta aagaagaaga gatagaaagt ataaaagaga
     1021 tttggagtta ttgttttaac gatacggaga gttttatgaa gtactatttt aatgataaat
     1081 ataaatctga aaatactgta gtagctttag atgaaggaaa aattatttct tctcttcagc
     1141 taaatcagta taagttatta ctaaatagca aggtatataa tacatcatat gtagtaggag
     1201 tttctacact tccagaggga agaggagctg gatatatgaa caaagtaatg aaatttactt
     1261 taaacgaatt gtataaaaaa gggcaattag tttctatatt gatgcctatt gactatagac
     1321 tttatagaag atttggatat gagcattgtt acgaccagat tgaatatact attaacacag
     1381 atgatttaaa aaattttaag tcaagtggaa aaatgataaa gtctaattta tcacaaattg
     1441 atgatttgat acgaatagat agaacttttt taaatgaggt taatggtaat gtgttaaaag
     1501 atgaacatta ttatgaaaac ttatttaaag agattcaaag tgaagatgga tttttataca
     1561 ttcatgaagg aaatgaaaaa gatggatata tagtttattt tcttcaggaa gataaaatgt
     1621 ttgtcagaga gttattttat aaaaatatag atgcccttaa atcaatgctt aaatttatat
     1681 ataatcacaa tactcaatgt aagatagtta ctatatctac tcctacaata gataagataa
     1741 gatttatatt agacaaccct aaagatagtg atataaaaat aaaacctttt atgatgggaa
     1801 gagttataaa tgtaaagaag tttattgaag atatagatat tgagaaggat attaatagct
     1861 catttaattt attaatagaa gacaagttta tagatgaaaa taatggtcta tttaaaattt
     1921 ctatacagaa taagaaggta tctgtagagc agttagataa aaaaggtgca gaaaagcctc
     1981 aagaagattt tgatattaaa ttagatataa ataccttaac acagttatca ttttcttata
     2041 tagatgtaaa tgaagctatt ttcttaaatg atataaaaga tgtttcagaa gaaacactag
     2101 aaacacttaa ttgtattttt tctaaaaaaa atacctatat aaatgaatat atataggtag
     2161 aatacgttaa aagattttaa aaatcttttg acgtattttt ttgtttagat aacaatttat
     2221 cttgtttaaa aattttctat ttgaaaataa tctaaataat atacttttat atgggagtga
     2281 ttataattca aataagaaga ccattattaa ttatattaat gtttgtggta gctatatcat
     2341 ttatcattac aaataacaca aaaaacgacg aagaacttaa tgataagata ataactatac
     2401 aaggggttgt caaaggaaaa attgaaaaga aaagatacaa tcagtacaag gtaggggttt
     2461 ttctaataaa tgattataca aaaaataaaa atttgaagat tggtcagaaa gttaatatta
     2521 caggtaaatt caaaagttta gataaaatga agtatgatga ttttgattat ggaaggtata
     2581 taaaaagtac tggatataaa ggtatagtgt atataaataa ctataaaatt attgataaaa
     2641 ataaacttta ctctttaata ggggaaataa agttttatat tagtaaaact tataggtact
     2701 tatataagaa aaactcagat tttataaatt ctatactatt agcagaagtt gaaaatttga
     2761 ctgaggaaca aaaagaaatt tttactagaa ctggaactag tcatgtaata tctatatcag
     2821 gtcttcatac aggaatatta tgtgtaatta tatcctttct attaagaggt ataaataaat
     2881 tgtataaatt attaa
//
LOCUS       DAEPOZ010000325         2865 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.326, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000325 DAEPOZ010000000
VERSION     DAEPOZ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.326"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            71..868
                     /locus_tag="KR440_003508"
     CDS             71..868
                     /locus_tag="KR440_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBH0700509.1"
                     /translation="MEKREKRIIISSLLSVSILMGLVSIYSILNKEDIILTVKGQEQK
                     VSSFKKTVEELLDEQGVKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            978..1568
                     /locus_tag="KR440_003509"
     CDS             978..1568
                     /locus_tag="KR440_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBH0700510.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNEETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFADFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            1634..>2865
                     /locus_tag="KR440_003510"
     CDS             1634..>2865
                     /locus_tag="KR440_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBH0700511.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVG"
BASE COUNT         1203 a          289 c          546 g          827 t
ORIGIN      
        1 atatataata taaggttaaa actgtaatga ataatattaa tttaaaattt gaacatggaa
       61 ggagattatt atggagaaaa gagaaaagag gataataata tcatctttat taagtgtatc
      121 aattttaatg ggtttggtaa gtatatattc tatattaaat aaagaagaca taattttaac
      181 tgttaagggt caagagcaaa aagtatcttc ctttaagaaa acagttgagg aacttttgga
      241 tgaacagggt gtaaagtata attctgaaga taagattaat ccaagtttag atacagaact
      301 aaaagatgat atgaaaataa aagttgttaa ggtaactaaa agtaaaaaag aagagattga
      361 aaaaattcca tttgatacaa agcatgtaaa tgatagtaat ttgttaaaag gaaaatctaa
      421 agtttatcaa gaaggtcaag aaggagaaaa aaaactagtc tataatttaa cttaccatga
      481 tggaaaatta gtcaaaaaag tcttatcaaa agaagtaata tctaaggagc caactacaaa
      541 aattataaaa tatggaacta aagaaaaagt actaatagca tcaagaggag caaatataag
      601 aggaggcaaa catatgaaag tggttgccac tgcatatgca ggagatacaa taacatctac
      661 tggtacaact ccaagatggg gtgttattgc agttgaccca cgtgtaatac catatggaac
      721 aaaagtatat atacctaaac tgggtatgac ttttgtagca gaagattgtg gaggtgcaat
      781 taaaggtaat agaatagaca tttttatgaa cagtgaagga aaagcttcaa attggggaag
      841 gaaaagtata gatatatatc tacattaaat tagtgaatag aataacttta cttgaaggac
      901 aaaaaagtat aaaatatgaa ttaactaaac tgttagatta ttcttaaaac ataaaaatat
      961 atctaagggg ggataagatg aaaatagtct gcttaggaga cagtttaacg tatggatttg
     1021 gagtatcacg aagtaattca tggacaaaca ttgtaaatga agagactcga ttagaaatag
     1081 taaataaggg tataaatggt gatacaacaa gtggtatgtt agctaggttt aatgaagatg
     1141 ttgtaaaaaa ctctcctgat atagttttta taatgggtgg cactaatgat tttattgctg
     1201 gtgcgggaaa tgaaattatt aattctaata taatggctat ggtgcatcag tcatattcaa
     1261 aaaatattat acctataata ggtataccat taaaaccaga tattccaaat gttagggaag
     1321 attggagttg ttttactgat tttaatatag tatctcaaaa cttagaatca tatagttatt
     1381 ggataaaaaa attttgtatg acatttaata ctaatttcgc agatttttat tcagaatata
     1441 ataaaaatat ggaactagaa ggatataaaa aattatattt tgatgggtta catccaacca
     1501 aagagggtca tagaataatg gcagacattt ttattaattc aattaataag tatatagaag
     1561 agttatagat tacattaaaa tgcttgttta tgcgtcagtt gcaataaata catacagtag
     1621 agaggggttt attatggatt atactaaaac ttatgaagag tggataaagg gttcatattt
     1681 tgatgaagat acaaagctag agttggagaa tataaaaaat aatgaaaaag aaatagagga
     1741 tagattctat aaggatttag aatttgggac tgcgggtctt agaggaataa ttgaagcagg
     1801 aacgaataga ataaataagt acacagttag aagagctact tttggattgg caaactatat
     1861 attagaaaac acaacaaaag aagaaacaag tagaggagta gtaatagctc atgataatag
     1921 acacaagtct agacaattct gtatagaatc tgcaaatact ttggcagcat gtggtataaa
     1981 agcatatata tttgatagtt tgagaactac tccagaatta tcttttgctg ttagaagctt
     2041 aaatgcaatc gctggtatag tcataacagc aagccataat cctccagaat ataatggata
     2101 taaggtatac tgggaagatg gagcacaagt tatgccagag attgcaaatg caataacaga
     2161 gaaagtaaat agtatacatg attatagtac aataccaacc ttaacggaag ctaataaaaa
     2221 tttggtagtt ttgttggacg aatcacaaga tactaaattt atagaagcag taaaaagcca
     2281 aattataaga aaagatttag ttaaaaatgt aggtaaaagc tttaagatag tttatacacc
     2341 tctttgtgga acagggaatg ttccaataag aagagcattg aaagaagttg gttttgaaaa
     2401 tataattgta gttccagaag aagaaaatcc agacccaaat tttgcagggc ttgattatcc
     2461 aaatccagaa gagaaaaaag ctttaaatag aggtatatta ctcgcaaaag aaaaaggagc
     2521 agaccttgta atagcaactg acccagattg tgatagagta ggtgtagcag taaaaactac
     2581 tacaggtgag tatgctttac ttacaggaaa tcaaattggt ggtatgctga cacattatat
     2641 aatagaagga ttaaaagaaa ataacaaatt aaaagaaaat cctacaataa taaaaaccat
     2701 agttacatca gaatttggag cagatatagc taaagcaaat aatgttgatg tattaaatgt
     2761 tcttacaggt tttaaattta ttggtgaaaa aataaaatta tttgaacaaa ataaaaatag
     2821 aagttatgta tttggatatg aagaaagtta tggttactta gttgg
//
LOCUS       DAEPOZ010000326         2864 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.327, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000326 DAEPOZ010000000
VERSION     DAEPOZ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.327"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..248)
                     /locus_tag="KR440_003511"
     CDS             complement(<1..248)
                     /locus_tag="KR440_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0700512.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKK"
     gene            515..832
                     /gene="trxA"
                     /locus_tag="KR440_003512"
     CDS             515..832
                     /gene="trxA"
                     /locus_tag="KR440_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBH0700513.1"
                     /translation="MVKIINNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            complement(1039..2313)
                     /locus_tag="KR440_003513"
     CDS             complement(1039..2313)
                     /locus_tag="KR440_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBH0700514.1"
                     /translation="MQFMDVSKYIEGANLKVKTKNGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPWLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSAIYVKNATEGINF
                     LAKFFKDENPDKFIITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGISPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
BASE COUNT         1042 a          341 c          389 g         1092 t
ORIGIN      
        1 tttttcttat tttttaataa ttcattaggg tttatgggta tgtacttaat agggtcagtt
       61 ccaacattga taattatatt attgtttaat agctttgtta tattttcata gacttttgat
      121 tgtggtacac ctgataattt acttatttca tatcctgtta aagggctttc ctttaataat
      181 gatatgtata tttgagattc gtatttagta aatcctatat tttgtaattc attaattata
      241 ttatccatat agaaactcct ttgataaaag attagtaact acctatgtag ttattaattt
      301 gattttataa tttaagggag aaaatgtcaa ctatattaag aaaaaaataa aatatatcag
      361 aaatatttag tcttataaat atttaatctt ataaatctat ttatcataat attaaatcta
      421 ttaaaaatag gcggttacaa aaaagtgcct aattgaactg ttatataggg cgtagtatat
      481 tagtatcaag aagataataa aggagatgta tattatggta aaaataataa ataataatga
      541 atttataaat gaagtagaaa ataaagatgg tttagtagtt gtagatttct ttgcgacttg
      601 gtgtggacct tgtaagatgc tatcaccaat atatgaagct ttaggaaatg agatggttga
      661 aaaagcaaac ttcttgaagg tggatataga ccaaagtata gaattagctc agaaatttga
      721 agtatctaca gtaccaacta tgctaatatt taaggatgga aaacctgtag atagattaat
      781 aggttttatg cctaaggaga atttaaaaaa caaaattgaa tcatatatgt aattaaagta
      841 aattgaagta attaaaataa atatcatata taaacaaaaa aactcattgt aatgagtttt
      901 ttatttgtat agtgtaaaat agtttgtata gttacaaatg agtgtctaat tgctaatgaa
      961 attgactgtt gatataattt gtttttttgt gaaaacaaaa ctactctcta tttattgata
     1021 aaaaagaata gaaaaagctt atttaaaagc caaagtttta atagcatgta taaagctatc
     1081 cacctcatca aaagtattgt acattcctaa gcttatccta acaagaccta caggattttc
     1141 cctcataaat ctgacgtatg cttcttttgg acttatacct aataattttt gtacataaat
     1201 atctgcacct actactccag ctccagtttc tattccaaag ttatcaacta aatattttgc
     1261 tacatctttg taatacatat tacttatatt aaaagatata tttgggataa ttacattatt
     1321 tgatttatct ccatatatta ttatattagg tattttactc atttcttctt tagcatataa
     1381 aaataattct ctttcgtatt ttgaaattct gtttagacca attttattta aagtgttcag
     1441 tgcttttccc attgcaagag ttccaaaata ctctgggtat cctgcttcaa aacgttgggg
     1501 tacgtttgca tatagtattt tgttatcatt tacaaatttg gtagaacctg agccatatag
     1561 tacaggatta aatttttcaa agaatttttt aggtcctacc agagctcctc catttagtgg
     1621 agaataacat ttatgagcac taaaaacaac aaaatctata tgctcatcac attcatgtgg
     1681 catcattgaa aaaggtttat gctgaattac ttgcacaata tccaccaata tctttgcacc
     1741 atacttatga gctattcttg ctatttcata tataggagtg gttatacctg tgacattact
     1801 agctcctgta actgtaacga gagatacatt accagcataa tttttaagta tattctctaa
     1861 ttgacataaa tcaagttctc ctttttctgt aattccaaca acttttgtag gaaaattaac
     1921 tttaaaaggt aggtaattag ccatatgctc cattgctgtg gtaataataa atttatctgg
     1981 attttcatct ttaaaaaatt ttgctaagaa gtttattcct tctgtagcat ttttgacata
     2041 aatagcagaa tccttttctt cgtctccatc aataagtttg aggatagtag agcgtacatt
     2101 ttcatatttt aaagtatttt tttctgagat tattcctgga gcattaatat aagtaagcca
     2161 aggaatctca cagtttaagt tatccactac gttttttaaa actaatggag tggcagagtt
     2221 gttaaaatat attcttttaa cagtttcacc atttttagtt ttaactttaa ggtttgcacc
     2281 ttcaatgtat ttagatacat ccatgaattg cataaaaaaa ccccttatct aataaactat
     2341 tctgtttttt attaaaaaag ctcaagtttt aatcaagagc ttgtttaata tatgttatgt
     2401 ttttataagg ggttttgtta attaattctt gttgagatgt ttattaaatt acacgaaaat
     2461 gagctacagt tgggggaaac tgtagctcac ttgattttgg gggagatagg ttgaacttat
     2521 tgtttatccg tatcatattt ttagtatagt cacttatttt aatttatata cattttttta
     2581 taaattttac ataaaatgtt aaacactaat tagaaataat tatgaatatg actaataaag
     2641 ttgtaaaaat agtaaagaaa ttgattagaa cataatggaa aatatattgt atataattag
     2701 aaaaaaagat tttatatgtt ataaaagatg tacaagtata ataattttat aaaaaataca
     2761 aaatataata caaataattt tataaaaaaa attataaaaa aatttaattt aatttatata
     2821 aattaaaaat tatattacca atgtatatat ttgagaaaag atta
//
LOCUS       DAEPOZ010000327         2860 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.328, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000327 DAEPOZ010000000
VERSION     DAEPOZ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.328"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            223..1443
                     /locus_tag="KR440_003514"
     CDS             223..1443
                     /locus_tag="KR440_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBH0700515.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKADYIFGLHVMPHIETGLIETKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISSTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            1480..2829
                     /locus_tag="KR440_003515"
     CDS             1480..2829
                     /locus_tag="KR440_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH0700516.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYSLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWFIRIISWSTL
                     AVGFATALGSFWPESATEYKGCIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFVIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     IALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFLGNYGKVFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPMVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
BASE COUNT         1083 a          361 c          465 g          951 t
ORIGIN      
        1 ctattttttt tatataaact agcatataaa atatattttc ttcaaaataa tattttttac
       61 tgaaaattga aactatgcaa tcatttttta tactatacat atagataaat gattatattt
      121 attcaatatt cttataaaaa acaaaattct ccatcgaaaa ttatggcata ataattgctt
      181 attatattaa taaaacaata aaaagcaatt ggaggaataa aaatgcaaca ggctttgaaa
      241 ttaaaatatc aaaccaaaga attaggaaaa gattttgtaa tagaaagttg taattctata
      301 aaaccatggc ttataaacat aagaagagaa ttacataaaa ttccagaatt agctcttgaa
      361 gaaaatctta ctaaacagaa ggtaatttct tatttaaaag aaatcggaat tgattatatg
      421 gaatttacaa aacacaatgg aattatggca tatattttaa aagaaagtgc agataaaacg
      481 atttgtataa gagcagatat ggatgctctt cctattgaag aagaaaacaa cataccttat
      541 aaatcaatac attctggaaa aatgcatgct tgtggtcacg atgcacatac aaccatgcta
      601 cttggagcat gtaaagtttt acattcaata aaagataaac ttaatgtaaa tgtaaaattc
      661 ttatttcaac cagcagaaga aggttttgga ggagcaaaat ttttagttga ggatgggtgt
      721 ttagaaaacc ccaaggccga ttatatattc ggacttcatg ttatgccaca tatagaaaca
      781 ggccttatcg aaacaaaata tgatacatta aatgctagtg tagacactat aaaaataagt
      841 atcaaaggta aaagagctca tggtgcatat cctgaaaatg gtattgatgc aatagttaca
      901 gcttcacaaa ttgttacatc attacaaact ataataagta gaaacttaga accaaataat
      961 gctgccgttt tgactatagg taaaatatat ggtggtgatg cacacaatgt aatatgtgaa
     1021 gatgtaaaac tagaaggcac tttaagaacc ttaaatagca aaacaagaaa ctttatgatt
     1081 gataaaatag ctaaaattgt tggagacact gcatctgcat ttggttgtgt tgggacttta
     1141 catgtaagtg atgaaaacta tcctgctgtt ataaatgaaa aagaattagt agatacagtt
     1201 atatcaagta ctaaagaatt acttggagag gaaaaattta tattgagacc aaacccgtct
     1261 ttaggtggtg aagacttttc tttttacaca gaacattgta aaggagcatt cttccattta
     1321 ggatgtaaaa atgaggaaaa gggcttaatt tcacctcttc atacctctag cttcaatata
     1381 gatgaagatt gtttaccaat aggtgttatg atgcatgtaa tgaatacttt gtattttaat
     1441 tagtacagta attaataaaa gaataggaga taaattaata tggaaaaagc taagaaatta
     1501 ggtctattta gtatgatatt attagggatt aatagcataa taggttcagg tatattctta
     1561 ctaccaggta aagtttatag tctagcaggt caaaacagta tgtttatata catttttgct
     1621 acactcttag ttctatcaat acttttatgt tttgcagaag ttggaagcat gtttgataag
     1681 aatggaggag catatctata ctccaaaaaa gcttttggag attttatagg atttgaagtt
     1741 ggtacaatgt cttggtttat tagaattata tcatggtcta ctctagcagt aggatttgca
     1801 acagctttag gttcattttg gccagaatct gccactgaat acaaaggttg tatagctgca
     1861 atactcgtaa cactactttc aataaatagc ttatttggaa ttaaaagcac aaagataatg
     1921 aataatgtaa taactatagc taaattagta ccattaatag tttttgtgat agttggaata
     1981 ttctttataa aatttgtaaa tattgttcct tcaggaaatg tagttaactc tagtatgggt
     2041 ccagccatta tattagtatt ttacgcattt accggctttg aatcatttat tgtggcaagt
     2101 ggagaaatgg aaaatcctaa aaaaaatctt ccaatcgcat taataaccac aatatttata
     2161 tgtgcaataa tttatatatt aattcaaatt gtctgtatgg gtatacttgg agatagacta
     2221 tttgaaaaca gtattccaat tgcagatgct tcaagtgtat ttttaggcaa ttatggtaaa
     2281 gttttcatat ctgtagcaac attaatatct atatttggta taaatatagg ttcatcaata
     2341 gtaactccta aatgtggttc ttctctagca gaagaaggtt ctcttcctgc atttatagga
     2401 aaaacaaata aatatggtgc tccatatgta gcaataataa tatctttaat ttgttgtata
     2461 ccacttgtgc taactggaag ttttgaacaa cttgcagtaa tgagtgtaat tgcaagattt
     2521 gctcagtaca taccaacttg tttatcagta atagttttaa gaaaaagaac agatgttaag
     2581 gcctctttta agattccatt tggtcctgtt ataccaatgg tagctatttt aggtagctta
     2641 tggttgttac aacaagcttg ggcagaagat atttcaaaac ctataactca aaatagagtc
     2701 ttaataggat taggggcaat gttgttaata gctcctctat atatatttat gaaaaaaaat
     2761 aaggaaatcg agcataaaaa taaatcagaa gattctaaat tatatatttc gagagaaaaa
     2821 attcaataat attttatact attacttctc ataaaatata
//
LOCUS       DAEPOZ010000328         2842 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.329, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000328 DAEPOZ010000000
VERSION     DAEPOZ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.329"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            100..2328
                     /locus_tag="KR440_003516"
     CDS             100..2328
                     /locus_tag="KR440_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBH0700517.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
     gene            complement(2566..>2842)
                     /locus_tag="KR440_003517"
     CDS             complement(2566..>2842)
                     /locus_tag="KR440_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBH0700518.1"
                     /translation="QLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISLQDLINNID
                     EIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
BASE COUNT         1069 a          382 c          394 g          997 t
ORIGIN      
        1 attaagacaa aattttaact ttactatttg tgtaaaaagt aatataattt aaaattgatt
       61 ataaatctaa cttttttctt tatcaaaata ttttaggagg tgagacattt gaaaaaaact
      121 aatagtttaa aagaaatttt aaactggaat aatatcaaga atttaagacc ttcaaagggt
      181 ttaaaaaata tattagaaat ttttaaaagt gacctaaaag atatacgtgg caattatgcc
      241 gtacttgtaa tagttttagg tcttgctttt ttaccatctc tgtatgcttg gttcaatata
      301 aaggcatctt gggaccctta tggaagcact gctaatatgc ttgtagcagt aacaaatgaa
      361 gataaaggaa atgaaatagc aggacaaaag attaatgtag gagaccaatt aattgaacaa
      421 ctcaaagata acaagagttt aggttggcag tttgtagatg aaaaaactgc tcttgaaggt
      481 gttaaaaaag gtacttatta tgcatcactt aagattccaa aaaattttac aaaagatttg
      541 acatctttaa ttacagatga tgttaaaaaa ggcgaaatta tctatactgt taatgaaaaa
      601 ataaatgcag ttgcaccaaa gattacagat aaaggtgctt ctacaataca actacaggtt
      661 aatcagacta tagtaaaaac agttagtgaa atcgttcttg gtatatcaaa cggagttggt
      721 gcaggaattg aacacagtct tccaaaattg acaaaagttg aaaattccct tattgacctt
      781 caaaagagat ttgaccatat aaataagact gtggatttag cttctgatgc tacttcaaaa
      841 ttatctgata cagttaaaga tattaaatct gatttaccta caattaaaaa gactctgaat
      901 gatacaaaac tattatcaag tgacttaaaa aaattcttag aggatacaaa tgataattta
      961 gacgaacttt ctcctcttat taagtctgac ttaaatttaa tggttgacct ttcatcttct
     1021 gcttcttctc ttacattaaa tttaattgat gcagtaaata gtggcagtga agatgtacct
     1081 aagttgatag ataatttatc agaaaaactt tctaatttgc aatctttaaa cgatactttg
     1141 gtggactttt taactaaatt aaaccaactt acatcaaata atagattgga tgatgtaata
     1201 gataatcttg aagactctag caataaaata gattcatcta tatcaacatt aaatgatatt
     1261 aaaaacaaag ttatatctgg acaacaacca tctataagtg ctttgaataa tgttttaagt
     1321 ctatcaaatg gtattggaag aattaattta aatattttaa ataattttga ctcaaaaata
     1381 tcaaaaccta taaatagtat atttgcaaac agtataaaag tagcaaatga tataattact
     1441 gttttagata aggcagaagc aaaacttcca aaggttgaag aaatacttac tacttctcta
     1501 aaactttcag gaaatgcaca ggaaagtata tcattgataa gagaaagact tcctttagca
     1561 aaaggaatgt tggatgattt gattgatact ttaagcaaaa ttagtaatgg tgaagatatg
     1621 aaaaaattag tcagcttgct tgaaagtgat atattgacaa agtctaattt cttaaaagaa
     1681 cctgttgagg tgaaaacaga gaaattatat ccaattgaaa attatggttc tgcaatgaca
     1741 ccattttata gtgttctttc tacatgggtt ggtatattac ttatttcagc actattgtct
     1801 acatctgtac atggaaacta taaaccttat gaaatttact ttggaagagg tcttactttt
     1861 ttaagtatag ctcttgtcca aggttttata ataggaactg gagatatatt attgttagga
     1921 gtaacagcaa aacatccaat tttattgata gttctgctta tgtttactag tatggtattt
     1981 aactttatag tgtattcttt agtttctgta tttggaaatg taggaaaagc aattgcaata
     2041 gtactattag tcgttcagat tggttcttct ggtggtacat tccctataca agttacacct
     2101 acattcttcc aaagaataaa ccctataatg ccatttacct atgcaatagg tgctacaagg
     2161 gaagcagttg gtggtatata tgctccaaat ctaactaaag atatactagt tctattacta
     2221 tttatgattc tgtctatttg tttaaacgta cttctaaaag gacctataaa tagagttata
     2281 aaaccattta atgataaatt tggcgatagt aatttaactg gtcattaaaa aattaattct
     2341 gtctttaaat atattttttt aataatattc taagttcata ttaaaaggta gtagctaaaa
     2401 agctactacc tttttccaac ttaaaaacta atatttacta caaaaactgt catagaaaaa
     2461 agcgaaaaaa ttcgttcttt cctaaattta tcttataaat atttagatat attcctactt
     2521 aataaacaga gctatacata gtatttaatg tataacttac cttatttaac tcatataatc
     2581 aattattcct gaatttaaat tatataaatg ctcaaaacct tcttcagcta acaaattaca
     2641 tgcagtaaca cttctatgac cacttctgca ataaactact actttcttat ctttataatc
     2701 gtaaatttca tctatattat tgattaaatc ttgtaaagat atatttatag cattttctat
     2761 tttatattcc ttaaattcat ctttagttct tacatctaat aataaaacat ccttttcatt
     2821 tttcatcatt tctttaagtt gc
//
LOCUS       DAEPOZ010000329         2832 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.330, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000329 DAEPOZ010000000
VERSION     DAEPOZ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.330"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..162
                     /locus_tag="KR440_003518"
     CDS             <1..162
                     /locus_tag="KR440_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700519.1"
                     /translation="KITKTFGFSCFICSPVGIIYEDMKNINIKIEHTSINELNPEELF
                     VNTSLLLYY"
     gene            452..994
                     /locus_tag="KR440_003519"
     CDS             452..994
                     /locus_tag="KR440_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBH0700520.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
     gene            1319..2812
                     /locus_tag="KR440_003520"
     CDS             1319..2812
                     /locus_tag="KR440_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBH0700521.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTVSFAIPLCENINVDCNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINKGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNILVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKVNELKDIEKSRITHGKILFEEYSKG
                     ILKKVSKRKKVDKNSYKSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
BASE COUNT         1261 a          219 c          466 g          886 t
ORIGIN      
        1 aaaataacaa aaacttttgg attttcttgt ttcatttgtt ctccagttgg aattatttat
       61 gaagatatga aaaatataaa tataaaaatt gaacatacat caataaatga attaaatcca
      121 gaagaattat ttgtaaatac atcactttta ttgtattatt aagtatttta aagctaaaac
      181 ttaaacttat taaaatagca tttggttaaa ggaaaataga tattgatata tttataatat
      241 atataaatga ttaattaaac aattataaat aataagtata gtataatatt taaaaaatat
      301 agacttatga taaaataaga caaatatgtt aaaaaaatga taatcacgga gaacttttac
      361 cttcttcaat taatatatta ttattagtaa taactttaaa attatattac tattatataa
      421 aaattggctt aaaagaaggt gaaaaaagta aatgaaaata tgcataacag taggtcatag
      481 tatactaaaa ggtggtaaaa gtacaggtgt aaatggtata gttgatgaat acaattataa
      541 taaaaaatta gctcctatgt tagctgagat gcttataagt caaggtaata cagtagatgt
      601 aataatttgt ccagaaaggc actttatgtc agaaagagaa gaatttttct atagagttcc
      661 aaaagtaaac agtggtaaat acgatttatt agtagagcta catttaaata aagctgatgg
      721 aacacaatgt ggtacagaag tgctttacta tggcactgaa ggattagaat atgctaaaag
      781 agtatctaat agacttggag aattatttga aaatagagga gctaaaaaaa gagaaaattt
      841 atatatattg agaaatacaa atccagttgc aatacagatt gaaagttttt tctgtgataa
      901 tgtgaatgat tgtaacaaag caaatgaatc aggttatgat tatatagcac gattgattac
      961 agaaggtata ttgaataaag atataaatat gtgagattat ggtgatttag gtattgatta
     1021 atacctttta atattagata taaataaaaa tacaatgtat tagtataatt taaactttca
     1081 aaatatagat tagtttgatt attgacaaga gatacctcct aaatattgat ttatcagtat
     1141 atttaaggag gtatttaaat ataaaaattt aattgatttg ctactaaact ataggtttat
     1201 agagtatata tttttatctt cagtctaata aataatataa aaatagtaga accttgtatt
     1261 ataataatat aatgtagaaa aatatataga taagataaat ataataaggt ggtaatatat
     1321 gaaatataca tttgaaaaat ttaagttaaa tgaaaagata cttaagtcat tgaagagttt
     1381 aggatataat ataccttcta gagtacaaag agaagttatt ccaaaattat taaaaggtca
     1441 aaacttagta gttaggtcaa aaacaggaag tggaaagaca gtaagttttg caataccttt
     1501 atgcgagaat ataaatgtag attgcaataa tattcaagct cttatagtgg ttccaacaag
     1561 agaattagcc ttgcaagtaa aagatgagat atcagatata gggagactaa agaaggtgag
     1621 atgtagtgct atatttggca aacaatctat aaaagaccaa atagctgaat tgaaacaaag
     1681 ggttcatatt gttgtagcta ctccaggcag aatacttgac catataaata aaggttctat
     1741 taagttggaa aatgtaaagt atttagtaat agatgaggca gataaaatgt ttaataaagg
     1801 ttttgttgaa caaatggaaa aaatattatt aaatttacct aaggagaaaa tagtaagtct
     1861 tttttcagct acaatagatg aagagataaa atatatatgt gaaaaatata tgctagatta
     1921 cagtgttata aatatagaag aaaatgagag cgatactaat caaaaaacaa gacaaataga
     1981 tgacaaaata atcaaggcaa atggaagaga gaagtacatc cttttaaaag aattaatata
     2041 cagcgaaaat ccaaagagtg tgataatatt ttgtaatacc aaagagaaag tatctaaatt
     2101 atataataag atgagtaaag agggtttttt aataagagag ttacatgcag atttatcaca
     2161 agaaagaaga atttttgtaa ttaaggattt taaaaaccaa aaatttaata tattagttag
     2221 tagtgatgta gcgtccagag gaatacatat tgatgatatt tctttagtaa taaattatga
     2281 tgtgccacaa gataaagaaa actatataca tagaataggt agaactggga gaaaaggaaa
     2341 tagtggaaag gcaattacta tagtaactga aaaggatgaa aaatatatag agaatataga
     2401 aacgtatata ggatataaag taaatgaatt aaaagatata gaaaaaagta gaattacaca
     2461 tggcaaaatt ttgtttgaag aatattcaaa aggaatccta aaaaaagtat caaagagaaa
     2521 aaaagttgat aaaaattctt acaaaagtaa agatataaaa ctaaatatag aaagtgaagt
     2581 tgttaaatta tatttaaatg ctggtaagaa gaagaaaata agagttttag atatagttgg
     2641 tgcatttagt aatataaaag gtataactaa tgatgatata ggtgtaattg aagttcaaga
     2701 tttatgttca tatgtagata tattaaatta taagggagat ttgatattaa aaaaatataa
     2761 ggagatacct ataaagaaaa aaatggttaa agtgaaaaga gatatacgat aatttagtta
     2821 ttactgctct ct
//
LOCUS       DAEPOZ010000330         2754 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.331, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000330 DAEPOZ010000000
VERSION     DAEPOZ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.331"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            785..1186
                     /locus_tag="KR440_003521"
     CDS             785..1186
                     /locus_tag="KR440_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HBH0700522.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     gene            1183..2250
                     /gene="rsgA"
                     /locus_tag="KR440_003522"
     CDS             1183..2250
                     /gene="rsgA"
                     /locus_tag="KR440_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBH0700523.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYHDLYLARIISQEKNFYQAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKSGCTVALLGSSGVGKSSLINC
                     LLGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSE
                     YLAKKEKKFKNISKINKTIRR"
BASE COUNT          988 a          312 c          493 g          961 t
ORIGIN      
        1 tacaataata caatagaatt gcttattatt caatatactt atttgaaaat taaataaatt
       61 tacaaatatg aacgttattt tatataaaaa aaatagacta ttcatgtaga atatatggaa
      121 aaacttgatt gtaggttttg tatgaaaaga cagttgttat taatttaagt gagataaatt
      181 gagcaatagg cataaaatga gaaaacaaat aatagcaatt aatttttatt attaatacta
      241 ggtgaacatt aaattttaaa ataccactag attttataga ataagaaaat gagacaaaca
      301 taaatatatg aatatattaa attatacttg aacatactta tattttatga taaaatttta
      361 ataataatta ttagtgaagg agtgtcttgc ctgatgtcag agttatatat aatggtaacc
      421 aacaaataaa tagttggaga cgcatatggg ttctgtttaa tcagaatttt taagtgtatt
      481 tttgatacat ttgattgttg tgtcttactc tgcctttata gataactaac attagacaac
      541 aaataataat attacatatt actttgttaa attgtggtgt agtgggcttt aatctgctac
      601 acttttatta attttacaca gtattatttt gaatataatt atgttgaaat agaagatact
      661 aaattttcta gtccacaggg gttttctgtt tttgtgtagg taaatatgta taccacataa
      721 aaaggcagat ataaatacct gtggactttt tttgtagaaa atacaagata cggaggaaat
      781 aaaaatgaat tataaaaata gaaaacgagc ttattatcag gaaaattcct gtatggataa
      841 ctttatttgc aaaaattgtg gttgccttgt agttcctgaa ggagctggta cacaacatag
      901 aaatcattgt ccacattgtt tacatagtct tcatgtagat atcattcctg gtgaccgtgc
      961 tgcagattgt gatgctaata tggaacctat tggagtgtgg gtgagaaaaa atggtgagtg
     1021 ggcaattatt catcgttgta cacgctgtgg acatcttagt tctaatcgta ttgcagctga
     1081 tgataattca gtgaaattaa tgtcaattgc attaaaacca ctggcacagc cccctttccc
     1141 actagaaaga atcattgaaa agttggaaaa ggaggaaaca gaatgaatgg tatgaaacaa
     1201 atcaatttac aagactatgg tctaaatagg aattttatta ctcaaagtat ggaatatcac
     1261 gatttatacc ttgctcgaat tatctcgcag gaaaagaact tttatcaagc tgtatgtgta
     1321 aatggtgtac tgtttgcaga ggtttcagga aaactgcgtt ttgaggctac tatacaatct
     1381 gattttccca cagttggtga tttcgttatg ttggatcgtt gcaataataa aaatggtaat
     1441 gctattattc atcatgtgct tcatagaaaa agcttgtttg tgcgtaaggc tgctggaaag
     1501 gtaaatgagg aacaggtggt tgcagctaat attgacattg tttttttgtg catgtcactg
     1561 aataaggatt ttaacattca tagattggag aggtatctgt ctttaacatg ggaaagtgga
     1621 gctatcccag tagtagtact tactaaatca gacttatgtg acaatgtgca tcgtaaatta
     1681 caagtagtta attctatagc cttgggtgta gatgtagtag ttacctcatc tatagagcca
     1741 gatggttata aacagatttc taagtatatc aaatcaggct gtactgttgc attacttggc
     1801 tcttcaggag ttggaaaatc ttcattaatt aattgtctgc taggaggaga atttttaaat
     1861 acaaaggaaa ttcgcataga tgatgataaa ggaagacatg ccacgactag acgagagcta
     1921 tttttaattt caggtggtgg tattgttatt gacacaccag gtatgagaga gttgggaatg
     1981 tgggatgttt cagaaggttt agataaaagt tttgcagata tagaagctta tttcggaaaa
     2041 tgtcattttc gtaattgtac tcacacctca gaaccaggct gtgctatctg ggaagctatt
     2101 gctcatcaag aactttcgcc taaaagatgg gaatcctatc gtaggcttaa aactgaagca
     2161 gtatttgcag acaataaaag cgaatactta gcaaaaaaag aaaagaaatt taaaaatatc
     2221 tcaaaaataa ataagacgat tagaagataa gtgtagtttt tttgttcatt ctatgagtct
     2281 atatactatt aatgtattaa atttgccatt aatttgttac tttaatgtga ttttgatggc
     2341 aaatttggtt tgtggtatta tttaaagcaa tttattaatt tttttaataa agtttgattt
     2401 tttatatgat gatatatata tgtgataatg tatacatatt tgttacgata gtctccttta
     2461 taaagaggtc ttatgtaaga taagaaaagg catatttata ctaatacata atagagtata
     2521 tgcttatgta tgatagagat gtttcttaag agcaaaactg caatattgct aatttataaa
     2581 gtaaaaatat atgaattata aaatacaaat aagatattta taaaaattat tgttttaatt
     2641 tataataaaa attttttgtt gaaaacaatg ctcgaaccta taaaactatt aactaatatt
     2701 tgtaaatgta tacttttgat gataaaatat aattaagtta agttggtttt tata
//
LOCUS       DAEPOZ010000331         2751 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.332, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000331 DAEPOZ010000000
VERSION     DAEPOZ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.332"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            269..763
                     /locus_tag="KR440_003523"
     CDS             269..763
                     /locus_tag="KR440_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBH0700524.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIIALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTDGGMISWVKKDGKIIINGGFKDDPLADKIENLGMPYNG
                     SYLLMSSPGHEKYLELSILPEGEISKSGLDKLKNDYGNLIDITNDQNKINIVIKLLNN
                     KSNT"
     gene            1160..1310
                     /locus_tag="KR440_003524"
                     /pseudo
     CDS             1160..1310
                     /locus_tag="KR440_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897489.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1786..1974)
                     /locus_tag="KR440_003525"
     CDS             complement(1786..1974)
                     /locus_tag="KR440_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH0700525.1"
                     /translation="MTKYDFNFKLKVAKFYLNREGRDILIVKQYGVYDHSQVERWVNI
                     LGEGALKIKKFSILYGSS"
     gene            2136..2486
                     /locus_tag="KR440_003526"
     CDS             2136..2486
                     /locus_tag="KR440_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HBH0700526.1"
                     /translation="MIQKKIFMGFCGFLGFLSLRYFSSGNVTDLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNVELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            2488..2688
                     /locus_tag="KR440_003527"
     CDS             2488..2688
                     /locus_tag="KR440_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0700527.1"
                     /translation="MPKFICNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
BASE COUNT         1010 a          327 c          345 g         1069 t
ORIGIN      
        1 acattatttt ctgggtcctc tttttatttt catctgcttt taaataatat tattgacatt
       61 gatatgactg tagttttctt tattttaatc cattgcttat atcttttcta acaagaaatg
      121 tcattttaaa tcatttctat aaattgtaaa ataatagtat atttgtcata gaaagtgatt
      181 tttttgtcta tatatggtaa aatatagaaa gtagaaatca acaaaataaa aatttatata
      241 ttattaattt tatatggagg ggaaataaat gaaaaataaa aaaggattta ctctagtgga
      301 attattagta gtaattgcta taataggaat attggcaata atagcacttc cagcattatt
      361 taaaaatata gaaaaagcaa agatagctaa acttgaagct gatataagtg caataaaaag
      421 tgcatctctt agttactatg ctgatgaatc caagtatact gatggaggaa tgatatcatg
      481 ggtaaagaaa gatggaaaaa taataataaa tgggggtttt aaagatgacc cattagcaga
      541 taaaatagaa aatttaggga tgccttataa tggttcatat ctgttaatgt catctcctgg
      601 tcatgaaaaa tatctagaat taagcatact tccagaagga gaaataagca aaagtggtct
      661 agataaatta aaaaatgatt atggaaattt aatagacata acgaacgatc aaaataaaat
      721 aaatattgta ataaaacttt taaataataa atcgaatact taaatacaat aaatttgatt
      781 taataattac tgatgaaata aacaatcttg tttggagaaa tgaaaaaagc actccccaag
      841 aatagaatgc ttagttataa ctacagaagt gtattggcat acacttctat tttttattat
      901 atcacataat ttatgttgct aaaaatacct atttaagttg tttttaaatt gtgttgagta
      961 atattcttga tagtttatac attaatgaat ttcaaaaaaa taagcactct ttataataaa
     1021 aagtacttta gtgtatgtta atgtcctatc taataaaatt gtaacatgtc ttatgcttta
     1081 gtatgataat tttcattctc tttattgttg caacttatac tatagttttg ctaatttatc
     1141 tgcaaacaca acttctttat tgcatgtgta tcaaccaaaa acatcagaat acttacttaa
     1201 tacatctgca aaatcgtatc tttcatctat agtattctta tttgacaata taacaaaaaa
     1261 tgaatcgaga tatgcctttt ttataattag atatatttta taaattctag catatcattt
     1321 gttattttct gatatgctat tgatgtttta aatcttctat ttattttatt attttttact
     1381 tttccaattg tacctttata ggagttatat ttatgtgatt tatgaaaaaa tgctatgcat
     1441 ttaagttcta attttttgat aatacatcta actttctttt gattaataat gataccacta
     1501 tttttaatgt ggcttccctg aggcacacat cttttttaaa aactcaagct catcttttaa
     1561 aaatttattt tcatattcta attcttgttc tcttgttaat ttattttttc tttagtgttt
     1621 ttgtttttat tcattatggt cttcctttca ttttgaaaaa ggcatcaaca ccactatgta
     1681 aaaatttttg ttttcatgtt gctataactg tgctattgtt tattccaaaa tacttagcga
     1741 tttattgtac actatactta gttatcatca tatagtttat aacatctaac ttgaaccata
     1801 tagtatagaa aactttttta tttttagtgc accctctcct aatatattaa cccatctttc
     1861 aacttgagaa tggtcataaa ctccatattg ttttactatt aaaatatctc ttccttctct
     1921 attcaaataa aatttagcta cttttaattt gaaattaaaa tcatatttag tcatacataa
     1981 acatctcaag cgttagattt ttactctaac aattatggtg cagtacaaat attgtataat
     2041 actgattttc tattgacaaa agaatataaa agagatatat ttgtattata aagatacaaa
     2101 tatatctctt ttaaaattac taaggaggat aaaaaatgat tcaaaaaaaa atttttatgg
     2161 gtttttgtgg tttccttgga tttttaagtc ttagatattt ttcatctggc aatgttactg
     2221 atcttacata tattggattt tttgcttttt tctcaaactt tattatagca aaaataaatg
     2281 gtgataaagc agatgaaagg tatgtacaag atgaaaaagc agctatggca tttacaggac
     2341 agcttgctat tatagaatta ttcattttat ggtgtattac tatagtgtct aggaatgtag
     2401 aattaatgtg tgttttacta tcaattacat atgcaatcac tttaaatgtt tatgctataa
     2461 aactttatat tttagaggaa aaataatatg cccaaattca tttgtaacct aaaaaaatat
     2521 agacaattaa aagaattaac tcaagaacaa ttagcagaaa aagtaggagt tagaagagaa
     2581 accattatgc gtttagaagc aggaaagtat aatccttctt tgaaattagc aatagatata
     2641 tcaaaaatag tagatacacc tattgatgaa ttgtttattt ttgattagtt tttgcattat
     2701 taaaaatgtg ttttaaatac aaacttaaat tcaatcttta agtcactcct g
//
LOCUS       DAEPOZ010000332         2711 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.333, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000332 DAEPOZ010000000
VERSION     DAEPOZ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.333"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            152..916
                     /locus_tag="KR440_003528"
     CDS             152..916
                     /locus_tag="KR440_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBH0700528.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIIDFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(1201..>2711)
                     /locus_tag="KR440_003529"
     CDS             complement(1201..>2711)
                     /locus_tag="KR440_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBH0700529.1"
                     /translation="YKNKALYINDVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIP
                     ANLNANLRDYQINGLNWFKVLDYYKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVT
                     PTSLIYNWKNEFEKFAPDIKVLLIHGNKRDREKCFMELENFDVILTTYGTLRNDLEKY
                     SEIKFDYCILDEAQNIKNPVALVTESVKSINAENKFALTGTPMENNLLELWSIFDFIM
                     PGYLYSKAKFQELFINKEDNVKNLKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNK
                     EQKKIYSVYSKDIQDKMKDKNLKKDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIE
                     TCLEILRDSINENHKILLFSQFTSVLKNISKELDKYKIKYHYIDGKTNAKERLELVDE
                     FNNSMDKKVFLISLKAGGTGLNLTSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQ
                     VIKLIAKGTIEEKIIKLQESKKELINQFINGELSNEGVLKSLSDEEIIDLFN"
BASE COUNT          941 a          341 c          357 g         1072 t
ORIGIN      
        1 gaagtttcaa aaaacaatag aagttgttaa ggaaaggaat gttgacttta tctacattct
       61 aagctatact atttttaaat gtttatgaca atagtcattt taaatataag taataaatat
      121 atacctatag ggatattaaa ggaggtaata tatggcatat tttaactata acgacaaaca
      181 gtgctactat aatgaaattg gggatggtac accattactt tttttacatg gaaatacagc
      241 ctcttccaaa atgtttaatg atataatcga tttttataaa gatgaatata aagtaatatt
      301 aattgatttt gtaggtcatg gaaaatctca aatggtcgat aaattttctg ctgacttatg
      361 gtttgatgag gctatgcaag tgatatgttt tttagaagca atgaactata aaaaagtaaa
      421 cataattggt agtagtggag gagctcttgt tgcattaaat gttgcacttg agagacctga
      481 tttagtgaat aaggtaattg cagatagttt tgaaggagaa gttcctttag agtcatttgt
      541 acaaaatgtt aaaatagagc gtgaggcatc aaagcaagat gatggtgcaa aagcttttta
      601 tatttataat caaggagaaa actgggaaaa agttgtagat aatgatacag aagctatttt
      661 tgaacattat aaaactatag gaaaattttt tcataagcct ctagaaacta tgcaaccaga
      721 ggtgctttta acaggaagta gagaagatga gtttgtatct ctaatatcta gtgatttttt
      781 tgaaaatact ttttcattac ttcttgaaaa gattaaaaat ggtaaaatgt atttgtttga
      841 taaaggtggt catcctgcaa ttttatcgaa tggattagat ttttcaaatg tagcaaagaa
      901 atttttagag gaataattta accaaaattt acaataatga aaactgtaaa atggattgca
      961 aaacttacaa aagtgagtgt acatatactt cattattatg atgaaatagg attgttaaaa
     1021 caaaatattc taatagtcaa gttattacat ttgtatatgc aatatgtata gaaagagata
     1081 tcattagatt gtaaagttgg gattaaataa caatatgaaa ttttatttat tcagatattg
     1141 tagcttaact atataaatgt tagaataaat ttatatagtt aagctgtagg attaaataat
     1201 ttagttaaat aagtctatta tctcttcatc agatagactt tttaatacac cttcattaga
     1261 aagttctcca tttataaatt gatttataag ctctttttta ctttcttgta attttattat
     1321 tttttcttct atagtccctt tagcaatcaa ttttataact tgtactgaat ttttttgacc
     1381 aaatctatga gctctatcac tagcttgatt ttctactgat ggattccacc atgggtcaaa
     1441 gtgaattacc atgtcagcag atgtaagatt taaaccagtt ccgcctgctt ttaaagatat
     1501 taaaaaaact tttttatcca tactattatt gaactcatct acaagttcaa gtctttcttt
     1561 ggcattagtt ttaccatcta tataatggta tttaatttta tacttatcaa gttccttact
     1621 tatgttctta agcacagaag taaattgtga aaacaataat attttgtggt tttcgtttat
     1681 actatcccta agtatttcta aacatgtttc aatttttgaa ctttttttat tgtaatcttt
     1741 tactactata cttgggtcta aacaaagttg tcttagttta gttaaataag aaaatataac
     1801 tattttatct ttctttagat ttttatcttt cattttatct tgtatatctt ttgaatatac
     1861 actatatatc tttttctgct ctttattcaa ttcaacaaaa aagttttttt caatcttatc
     1921 tggaagttct ttcataactt gttttttact tcttctaaga ataaaaggtt ttattaattt
     1981 tttcaggttt ttaacattat cctctttatt tataaacaat tcctgaaact ttgctttact
     2041 atatagatag ccaggcatta taaaatcaaa tatagaccat aattctagta gattattttc
     2101 cataggtgta ccagtgagtg caaatttatt ttctgcattt atacttttta cagactcagt
     2161 tactaaagct actggatttt ttatattttg agcttcatct aatatacaat aatcaaactt
     2221 tatttcacta tatttttcta aatcatttct aagagtacca tatgttgtaa gtattacatc
     2281 aaaattttct aattccataa aacatttttc tctatctctt ttattcccat gtatcaataa
     2341 aacttttata tctggagcaa acttttcaaa ttcatttttc caattataaa tgagtgatgt
     2401 cggtgtaaca ataagacttt ttttattaga taatgataat aaaaatgcaa tagtctgtat
     2461 tgttttacca agacccattt catcagctaa tataccacca aatttataat aatctaaaac
     2521 tttaaaccaa ttgaggccat ttatttgata atcacgaagg tttgcattta aatttgcggg
     2581 aatttctaaa tttatgctat caaaatttct aaatttttca catatactgt ctagttcttt
     2641 tttaccatct ataaacttta aatttttact acgtattaca tcatttatat atagagcttt
     2701 atttttataa a
//
LOCUS       DAEPOZ010000333         2666 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.334, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000333 DAEPOZ010000000
VERSION     DAEPOZ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.334"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            310..777
                     /locus_tag="KR440_003530"
     CDS             310..777
                     /locus_tag="KR440_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBH0700530.1"
                     /translation="MKFLNVLIVVEDIEKSKKFYYDILGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDNFDTFVERLSTMKGIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEIGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
     gene            1949..2662
                     /locus_tag="KR440_003531"
     CDS             1949..2662
                     /locus_tag="KR440_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBH0700531.1"
                     /translation="MINIIICEGKKSERVILNDYINTFFYNYNFEYNIKEFENGGDLL
                     KKYPNNVDIIFMDIDLNDENGFNVSKEIKKFDESVRIIFVSSSNKYIKKGYEIGVYRY
                     LEKPLKYSLFKEAISSYLKECYKNEAILSIKCKCKIYFIKVDDILYLEVLKNDLTIHT
                     EKGIYTFKNSMKEIESNLEEHNFFRCHKSYLINLMKVNMLDIENNIVFINQNSIIVSR
                     YKIKLLREKLNSCLVDVII"
BASE COUNT         1135 a          158 c          368 g         1005 t
ORIGIN      
        1 atattaatat tattagtgta tattaatatt cccttgaaat taaaaaaaca tagatatttc
       61 agtaatctaa aatagtctac aaaaataaca gttttagcaa aattaataag aatacatata
      121 gatttaaacc taaaagtatg aacatgtctg ttttttacaa gattaaaatt tatattatat
      181 gataaaatta acttaactat ttcaataaga cttataattt ttttctttat actagacctt
      241 attgaaacct ttatagttta attctaatta tatattataa ataaagtatg tgtatattaa
      301 ggaggtaata tgaagttttt aaatgtttta atagtagttg aagatattga gaaatcaaaa
      361 aagttttatt atgatatttt ggggttaaag gtaatctgtg attttggaga aaatgttgta
      421 ttagaaggta atatatcatt acaagaaaaa aagctttggt tagaatttat taataagagt
      481 gacagtgaag ttaagttcaa tggaaatgat gctgagttat attttgagga agataatttt
      541 gatacttttg ttgaaagatt gagtacaatg aaaggtatag attatgttca tctagctata
      601 gagcataggt ggggtcaaag agcaattaga ttttatgatt tagatgggca tattattgag
      661 attggagaaa ctatgtcatc tgtgtgtaga cgttttttag atagtggtct ttcaatagat
      721 gaggtagcta aaagaatgga tgtaactgtc gaatatattg aatcagtttt agaatagatg
      781 gattatatta aagtaaaact atattttgta tagaagtaag ggctgtaagt aaacttgtat
      841 ggaaacaaat ttacttacag tttttgtttt tgtaagatac agaatataat atttgttata
      901 attttatttg gatttttaaa aagtgcatga ttttccttac atattataat agaatttaat
      961 atttattgga tgatttaaat ttagaaaaat ataagtatta gcttataaaa gataattata
     1021 cttactatca agctgtagaa tcatatatac tattttatgt aattatgtta aaatttaatt
     1081 gaacaaatta aaaatatagt gaaaattgta gttttagttt atttatttaa taaatatttc
     1141 aaataaaata atgtttaaat aagaaacata aataataata ttattattgt tcttaaagac
     1201 aaaaaatctt agcttaaaaa tttgtatcat ataaaatccc tacatattat ggaagtaatg
     1261 atacattata tgatatacac aataaagtat aaaagtattt acatgttttt tctatatatt
     1321 ttataagttt taggtatgga aaattactaa ataaaaatta gattaatgat tatgatagta
     1381 tagataaagt ttattctttt tattaataga aattgttgta taaaaataat aaaagtgagg
     1441 agagtgatat ttaaataaaa aaattttaat aagtattggt aatatagttt ttagggatga
     1501 ttattttatg taaaaagttg ttatttgtaa agataaatat agaaaatatt ataagtattg
     1561 atttagtgat aatctaaaag gtgagaattg taataaaaag agaacatatc cttcaaatgg
     1621 atgtaaactt ataaataaca ttattacaag agatttatta tacatatact gcaactatta
     1681 attagaattt atactgtatt tgtatttaaa taaaaacaca aataatatta ttgtaaaaat
     1741 ttataattat taaatattta aagtataaga tataataatt ataagtaatt tatgtataaa
     1801 tgtataaaat atagatatat ttagattaaa taataattat tattatacaa aaattttgta
     1861 ttaaaaaata tctttaaatt tgaaatataa taaatgttag atagaatttt ataaaattta
     1921 aaataaaaaa ttatataagg ggtgtaatat gattaatatt attatttgcg aaggtaaaaa
     1981 atcagagagg gtaattctaa atgattatat aaataccttt ttttataatt acaattttga
     2041 atacaatatc aaagagtttg aaaatggagg cgatttatta aaaaagtatc ctaataatgt
     2101 tgatatcata tttatggata tagacttaaa tgatgaaaat ggatttaatg tttcaaaaga
     2161 aatcaaaaaa tttgatgaaa gtgtaagaat tatatttgta tcctcatcaa ataaatacat
     2221 aaagaaaggt tatgagatag gagtttatag atatttagag aagcccttaa aatactctct
     2281 atttaaagaa gcaatatcat catatttaaa agaatgctac aaaaatgaag ctattttatc
     2341 tataaaatgt aaatgcaaga tatatttcat aaaggtagat gatattttat atttagaagt
     2401 tttaaaaaat gatttaacta tacatactga aaaaggaata tatactttta agaatagtat
     2461 gaaagaaata gaatcaaatc tagaagaaca taattttttt agatgtcata aaagctacct
     2521 tataaattta atgaaggtaa atatgctaga tatagaaaat aatattgtat ttataaatca
     2581 aaattcaata attgtaagtc gttataagat aaaattattg agagaaaagt taaatagttg
     2641 tttggtagat gtaataattt gaggta
//
LOCUS       DAEPOZ010000334         2629 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.335, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000334 DAEPOZ010000000
VERSION     DAEPOZ010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.335"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(18..1658)
                     /gene="bclA2"
                     /locus_tag="KR440_003532"
     CDS             complement(18..1658)
                     /gene="bclA2"
                     /locus_tag="KR440_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HBH0700532.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGATGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGANGTTGSTGPTGNTGATGANGITGPTGATGATGA
                     NGITGPTGNKGATGANGITGPTGATGATGANGITGPTGNTGATGANGATGLTGATGAT
                     GANGITGPTGATGATGANGVTGATGPTGNTGATGPTGSIGATGANGVTGATGPIGATG
                     PTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGLVGPTGATGATGV
                     AGAIGPTGAVGATGPTGADGAVGPTGATGATGANGATGPTGAVGATGANGVAGPIGPT
                     GPTGANGVAGATGATGATGANGATGPTGAVGATGANGVAGPIGPTGPTGANGTTGATG
                     ATGATGANGATGPTGATGATGVLAANNAQFTVSSSSLGNNTLVTFNSSFINGTNITFP
                     TSSTINLAVGGIYNVSFGIRAILSLAGFMSITTNFNGVAQNNFIAKAVNTLTSSDVSV
                     SLSFLVDARAAAVTLSFTFGSGTTGTSPAGYVSVYRIQ"
     gene            complement(2036..2575)
                     /gene="hpt"
                     /locus_tag="KR440_003533"
     CDS             complement(2036..2575)
                     /gene="hpt"
                     /locus_tag="KR440_003533"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBH0700533.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGKVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
BASE COUNT          614 a          648 c          412 g          955 t
ORIGIN      
        1 tcttttcatc atataaacta ttgtattcta taaactgata catacccagc tggagaagta
       61 cctgtcgtgc ctgaaccaaa tgtaaagctt aaagtaacag ctgctgctct agcatcaact
      121 aaaaagctta aacttacact tacatctgat gaagtaagcg tatttactgc ttttgcaata
      181 aagttatttt gggctactcc attaaagtta gtagtaattg acataaatcc tgcaagtgaa
      241 agtatggcac gtataccgaa agatacattg tatatccctc caactgcaag atttatagta
      301 ctacttgttg gaaaagttat attagttcca tttataaatg atgaattaaa tgtcactaat
      361 gtattattcc ctaaacttga agaagatact gtaaattgtg cattgtttgc tgctaacact
      421 cctgttgctc cggtcgctcc tgttggacct gttgctccat ttgctcccgt tgctccggtc
      481 gcccctgttg ctccggtcgt tccatttgct cctgttggtc ctgttggacc tattggacct
      541 gctactccat ttgctcccgt tgctcctact gctcctgttg ggcctgttgc tccatttgcc
      601 cctgttgctc cggtcgctcc tgttgctcct gctactccat ttgctccggt tggacctgtt
      661 ggacctattg gacctgctac tccattcgct ccagttgctc ctactgctcc cgttgggcct
      721 gttgctccat ttgcccctgt tgctccggtt gctccggttg gacctactgc tccatctgct
      781 cccgttgggc ctgtcgctcc tactgctccg gttggaccta ttgccccagc tactcctgtt
      841 gctccggtcg ctcctgttgg acctaccaaa ccatttgctc cggttgctcc ggttgggcct
      901 gttggaccta ccaaaccatt tgctccggtt gctccggttg ggcctgttgg gcctgttgga
      961 cctaccaaac catctggacc tgttgctcct actgctccgg ttggacctgt tgctcctatt
     1021 ggacctgtgg cacctgttac tccatttgct cccgttgctc ctatacttcc ggttggacct
     1081 gttgctcctg tatttcctgt tgggcctgta gcacctgtta ctccatttgc tcccgttgct
     1141 cctgttgctc ctgttggacc tgttatccca ttcgctcccg ttgccccagt tgctccggtt
     1201 agtccggttg caccatttgc tcccgttgct cctgtatttc ctgttggacc tgttattcca
     1261 tttgctcccg ttgctcctgt tgctcctgtt ggacctgtta tcccattcgc tcccgttgct
     1321 cccttgtttc cggttggacc tgttattcca tttgctcccg ttgctcctgt tgctcctgtt
     1381 ggacctgtta ttccatttgc tccagtcgct cctgtatttc ctgttggtcc tgtagaacct
     1441 gttgttccat ttgctccagt cgctcccata tttcctgttg gtcctgttat tccattcgcc
     1501 ccggttgctc ccgtatttcc tgttggtcct gttattccat ttgctccggt cgctccggtt
     1561 gcgcctctag gccccgtcgg tcctgttgga ccagtagcac ctgttggccc tgttggacct
     1621 acatcttgat ataaacttgg acctgaaata tcactcatat aattaatcct ccttttttaa
     1681 agttagagta ttactctata aatattgagt attactctat ataattatat gaatcgacaa
     1741 tttcttatgt gataatgtat ctatttgctt atatcaacat atatgctttg attcttaaat
     1801 ttcattactt ttttgtaaaa tcttttacaa ttattagtac tcttttacaa ttattaatac
     1861 aattaaaaaa ctcatttccc atcaattatt taaaattttt ttattatgtt ataaaaaata
     1921 gataattaaa ttgcataaaa ttaatttaat actactttaa ttacttttta aataaaaaaa
     1981 cctcaacacg attaataaaa atcatattaa ggcatccaca aaattatata attgcttatc
     2041 tatcttcatc agtaacattg aatatataag gaacatttct ataataatca ccaaaatcaa
     2101 acccatatcc aaccacaaac ttatcttcta tttcaaatcc acaataatct ggcactaaat
     2161 caacttttct tctgcttggt ttatccaaaa gaacacagca ttttaaacta gcaggatttt
     2221 tagcttttag atgttttaac acaaaatcca tagtaagtgc agaatcagta atatcgtcta
     2281 caaccaaaac atcatatcct gctatatcag tagtcacatc tgaaaccact ttaacctttc
     2341 cagtactttc ttcattgttt ccataactag atgtagtcat aaaattaact ctaagtggta
     2401 aatttatatt tctaactaag tctgcacaaa atataaagct accttttaat agagaaacta
     2461 ccataagatt tttatcttta taatcttttt ctataattgc tcctaattcc cttaatttat
     2521 ctttaatttt ttcttctgaa tacaatactt cccatttttt tgtttctata tccataaaaa
     2581 gacctccaat tgtaataatc cttcttaatt ttataagaga ttgctcact
//
LOCUS       DAEPOZ010000335         2608 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.336, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000335 DAEPOZ010000000
VERSION     DAEPOZ010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.336"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            245..1864
                     /locus_tag="KR440_003534"
     CDS             245..1864
                     /locus_tag="KR440_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBH0700534.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGNCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
     gene            2238..2432
                     /locus_tag="KR440_003535"
     CDS             2238..2432
                     /locus_tag="KR440_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBH0700535.1"
                     /translation="MINYMVEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYEM
                     TDAYRFETLCSDEKIRFKRI"
BASE COUNT          977 a          307 c          374 g          950 t
ORIGIN      
        1 ttaaattgat tgtacataaa atactaaaaa tctaagttca agtatttaaa ttgctatata
       61 tcataaattt tctaaaatta acaacaactt aaaaatttaa cattctctta acgtatactt
      121 aacaaaacat taatatttct attgacaaaa tttgttattc tatattatgg aaacaataaa
      181 ttttagttaa taaagtttat aattcagata aaaaaactat taatttctta agggaggaaa
      241 tatcgtgaat atagtaatta gccttatcgg tggacttgga ctttttcttt acggaatgag
      301 tttaatggga gaaggtttac aaaaatcagc aggagacaaa ttaaaaaaaa ttattgaact
      361 cttaacaagt aatgttgtta tgggtgtact ggtaggtaca gtcgttacag gtattataca
      421 aagttcaagt gctactactg ttatggttgt aggatttgta aatgcaggta taatgaatct
      481 aagtcaagct attggtgtaa taatgggggc aaatataggt actacagtta ctgctcaatt
      541 agtttcattt aatcttgaag gaattgcacc aattgcttta ggtataggta ttttattcta
      601 tttatttaca tcaaatcaaa aaacaaagca tttatctgag atacttatag gttttggtat
      661 attatttaca ggaatggagt ttatgaaaga tgcagtagct ccacttgctg aatataaagc
      721 attcactgat gcacttttat attttagtaa aaatcctgta ttaggtatat tggcaggatt
      781 tgccataact ggtattgttc aaagttcaag tgcatctatg ggtatgttaa tagcactagc
      841 ttctcaagga atacttcctc tatcatctgc tttacctatt ctttatggag ataatatagg
      901 tacttgtgtt acttcacttt tatcaagtgt tggtgcaagt agaaatgctc gacgtgcagc
      961 tgtaatgcat ttaagtttta atgtcattgg tacaataata tttatgcttg ttttaaataa
     1021 acctatatca gccatagtta ctcattttga cccaactgat acagctagac aaatagcaaa
     1081 tgcacatact ttatttaatc ttacaaatgt tataatatta ttaccatttt ctaaatacat
     1141 agttaaatta gcaaatagac ttatacctat taaagaaact gaatcagaaa tagtaaataa
     1201 tactaagtac ttagatgaaa ggatgtttag cactccatct atagcacttg gtaatactgt
     1261 acaagaagtt gtaagaatgg gtcataaagc tacaaactct ttggaacatt caattgcagg
     1321 atttttaaat aagagcaatg aagatattaa taaagctttt gagtcagaaa aagttgtcaa
     1381 taagttacag aaagatatat tgaactattt gttaaaactt tctaaagaac ctcttagaga
     1441 cgatgaacgc tttagaacag atttgctttt taatactgta aatgacattg aaagagtctc
     1501 tgaccatgca gaaaatatag ctgaattggc catgagtgtt aaggaaatga acattagttt
     1561 ttctgatagt gcgataagag aaatatacga aatatacaat aagacaatta ctaattttaa
     1621 ggatgcctta atagttttag atgttaaaga ttttgagtta gcaaataaag ttttagaagt
     1681 tgaaaatgaa gtaaattatt tagaaaaaac ttttagaaat tcacatatga ttagactaaa
     1741 taatggaaat tgtacaattg atgctggtgt actttatctt gacttactta ctaatctaga
     1801 aagaatttct gaccactcaa caaatatagt aaaacaagta ttaaaactta aacaaaaaat
     1861 atagtgatta tttttatata taatcttaat caatgagaag taagaagcta tagataatgc
     1921 ttatatcatt tatggcttct tattttttat gtaatactca aatttttata gtattctctt
     1981 attattaaac agcttctgcc tagaatattc catatatata tgacttcttt cataaaactt
     2041 gaactgccca taactaaagt cacaagtata cataactatt ttttaatcaa ttataaccaa
     2101 tatttatttt atttatcttc attttaattc ttcattcaat aatgtaataa gattattttt
     2161 tatttggata ctattatatc atctaaatat gttattatat atttggatat tttcatatta
     2221 ataggaggga ttctacaatg ataaattata tggtagaaat tatatatagc gaaaaaataa
     2281 gcgaagtaga aaacatgttg aaaattgaaa atatacaata tgaatttact tctcgtgatg
     2341 gctttatatt ttttggtttt tatgagatga cagatgcata tagatttgag actttatgtt
     2401 cagatgaaaa aattcgattt aaaagaatat aataaataaa aaaagtacat atttctaaac
     2461 tatttgtttt tcaaatttag gttgtgtttt aatgtagtgt tgatatcata aacaatttta
     2521 ataataaaaa agtaggaata ttatattaat atgctcctac tttttactca tatttattaa
     2581 tattatttat caaaactacc ctacaaac
//
LOCUS       DAEPOZ010000336         2598 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.337, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000336 DAEPOZ010000000
VERSION     DAEPOZ010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.337"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(868..1905)
                     /locus_tag="KR440_003536"
     CDS             complement(868..1905)
                     /locus_tag="KR440_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBH0700536.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKKTDSKKQ
                     KNIGISQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISTSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSAK"
     gene            complement(2002..>2598)
                     /locus_tag="KR440_003537"
     CDS             complement(2002..>2598)
                     /locus_tag="KR440_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0700537.1"
                     /translation="GLIKESSGQITLDSTTITNLAEYKRTQIVSRVFQDPSLGTCPSM
                     TVRENLSLALNKGKLLNLKKCLRHKTNNLEHLLEGISLDLKKYLDIKVQYLSGGQRQS
                     LSLIMSSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALQ
                     YGDRLIMLHKGEVVLDVKDKEKENLTVEEILEKFEYAV"
BASE COUNT          861 a          391 c          329 g         1017 t
ORIGIN      
        1 cccagtgcca taatcgataa aattaaactt tgcgtcatta cagatataat acctgacata
       61 aataaaacac tcctttgaaa atatttttat aattttaatt atttgctaat tttgtgtata
      121 aaaaaaccac atagtgtaca aaactatgtg gtaaaaacac gaataagcca catagtttaa
      181 tctatgtggc tcttatatag ttaaatgaaa actttataaa caatatagcc aacatagata
      241 caatcccaaa attttaatag tcttaagggg tctgtatcta tgttaaaacg taggtacaaa
      301 ccctaaaaat agtggctata ataataataa tattttctat tgtttaataa agtattcata
      361 attatctctc ccttttaata ttttaattag tcagaaaatt atatgtattc tcgacttgtt
      421 ataaattata aattatacaa taataaaagt caactgctaa atttaattaa aatactaaat
      481 aatgtatact cagaatactt ttaaattaat gtattgtttg caaaaaacgt tatcctaagt
      541 tataagtgaa tgtggaaatt ttatcaaaat atttactaaa tatggaataa ctagacatgt
      601 atatcaatca aatatcataa aaaaaagctg tctggagaat attaaaattc ttttggacag
      661 ctcaagtagt gattagaaag ttatataact ttcataatca gtcgtcaata tagacgactt
      721 ttatggaaaa tgaataactt tatatgcaat attcaaattg ctacaataag cacaattttc
      781 aaagggttag ggtggataag agtacttatt atataaagtt ttcatattac ctacttcata
      841 ttacctattt tttgaaatta ataagtttta ttttgcagat tttgcatttt catctgactt
      901 tatatacttt atattatctg ctgataactt ttgcatgtca agtgctttta gtgtatcttc
      961 gttaattatt atttcagttt catctaatgt agtaactggt atatcgccaa cagattttcc
     1021 ttctaatact tcaatagcaa gttctccagc cttataacct agtttttcat aatctattcc
     1081 acaacaagct aaagcaccag aagatacaga accttcttca gaggctatta taggaatctt
     1141 gttttcatta gcaactttag aaactattgg catagaagaa actattaaat tgtcagtagg
     1201 gacatataaa acatctattt tgccaactaa actagaaata gcttggttaa cttcacttga
     1261 agtgctgatt cctttttcaa ctacttcata attatttttc ttagcataat catgtagaga
     1321 atcaacctgg atttttgaat taacttcact agtattgtat ataaccccta ttttctttgc
     1381 ttttggagtc aatgttttaa ccaattctaa tgttttatca attgaaagat aatcagatgt
     1441 accagaaaca tttccacctg gtttttcaag agatttaaca agtccagctt ctacagggtc
     1501 tgtaacagca gtcattatta ttggaatatc tttagtagca ttataagctg cttgtgcaga
     1561 tggagtagat atagcatata tcaaatcttt tttgtcggat acaaacttac tagcaatact
     1621 ttgtgtagta ggcatatcat tttgtgcatt ttggaaatct atttttatat tatctccatc
     1681 tttatagcct ttatcttcga gtgctttgat gaatcctttc tttgctttat ccagagacgg
     1741 atgttccact aattgactaa tacctatatt tttttgtttt ttgctatctg tttttttatt
     1801 ttcatttgaa gcattggagc catcattttg agaacatcca gtaagcattg atacaccaag
     1861 tactcctgct ataattaaac ttaatatttt tttacttttt atcattttat tttcctccct
     1921 taaaatttaa taattatatt tgttcatata ttcatatgat agattataaa tatttctgat
     1981 taaaactaag attttaattt cttatactgc atattcaaat ttttctaaga tttcttcaac
     2041 tgttaaattt tctttttctt tatcttttac atctaataca acttcaccct tatgaagcat
     2101 tatcaaccta tctccatatt gaagagcgtg tttcaagttg tgagtaacca taagtgtagt
     2161 tatatttttt tctcttacga ttttatctgt taactctatt acttcatttg atgtttttgg
     2221 gtcaagtgct gctgtatgtt cgtctagtaa taagacctta ggacttgcta agctagacat
     2281 aattagtgat agtgattgtc tttgacctcc tgaaagatat tgtactttaa tatctaaata
     2341 tttttttaag tccaaagaaa ttccttcaag taagtgttcc aaattattcg ttttatgacg
     2401 aagacatttt tttaagttca ataatttccc tttattaagt gctagagata aattttctct
     2461 aaccgtcata gaaggacaag ttccaaggct tgggtcttga aaaactctac ttactatttg
     2521 agttctttta tactcagcta aatttgttat agtagtacta tctaaagtaa tttgaccaga
     2581 agattctttt ataagccc
//
LOCUS       DAEPOZ010000337         2572 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.338, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000337 DAEPOZ010000000
VERSION     DAEPOZ010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.338"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..1394
                     /locus_tag="KR440_003538"
     CDS             <1..1394
                     /locus_tag="KR440_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBH0700538.1"
                     /translation="TVLISGFIGKIQIPEYILDYIKSDIILNIIYMIFLLIIYISVIR
                     WIFSIHEITLNTENFKRARKESVNLIKRKIIRTIIYSLILSLVVAIIGYAIYHSGIIF
                     IGIWTKYCTDMEFLKNVFINRCIAFEEYFRLILSIFIFILDIGFISATYYQYKGVNIS
                     KNNIKKEKKSISKIVFNLVLILLISYIESVAFSSHINGMFNTSFSFYTTAIAHRAGAE
                     MAPENTLAAIEEASNAKADYAEIDVQETKDGELIVLHDSNFKRTTGVDKNVWDVNYNE
                     VKTYDAGSFYYYGKGYYDEKFVGEKIPTLKEMIKYSKGKVKLLIEIKLNGHEKGDIVK
                     KVVQLIKENNFENQCIVASMDKTILQKVKKLDENLLTCYFMAIAYGDFSKLNYVDIYG
                     IESTFVNKTVVEKIHKMNKKIFVWTVNSDSLIEKMLDLNVDSIITDNPYLIESAIYWK
                     KNGFISQVSDYLF"
     gene            1716..2300
                     /locus_tag="KR440_003539"
     CDS             1716..2300
                     /locus_tag="KR440_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBH0700539.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKDLVRDYNQTDSSDLEK
                     KEKILNELLGGKDKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNVKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT         1006 a          247 c          410 g          909 t
ORIGIN      
        1 atacagtttt aatttcaggg tttataggaa aaatacaaat accagagtac atattagatt
       61 atataaagtc tgatataatt ttaaatataa tctatatgat ttttctttta ataatctata
      121 tttcggttat tagatggatt tttagcattc atgagataac tttaaataca gaaaatttta
      181 aaagagcaag aaaagaaagt gttaatctta taaagagaaa gattataaga acaattattt
      241 attcattaat attatcttta gtagtagcta ttattggata tgcgatttat catagtggaa
      301 ttatatttat aggcatatgg actaaatatt gtactgatat ggaatttttg aagaatgtat
      361 ttataaatag atgtattgca tttgaagaat attttagatt gattttatca atattcattt
      421 ttatattgga cattggattt atctcagcaa cttattatca atataaagga gtaaatattt
      481 ctaaaaataa tattaaaaaa gagaaaaaat ctatatcaaa aattgttttt aatttagtat
      541 taatattatt gatttcttat atagagtcag ttgcattttc ttcacatata aatggcatgt
      601 ttaatacgtc attttcattt tatacaacag caattgctca tagagcagga gctgaaatgg
      661 ctccagagaa tactttggca gcaatagaag aagcttcaaa tgctaaagct gattatgcag
      721 aaatagatgt tcaagaaact aaagatggag aacttattgt acttcatgat tcaaacttta
      781 agagaactac aggagtagat aaaaatgttt gggatgtaaa ttataatgaa gtaaagacat
      841 atgatgcggg aagcttttat tattatggaa aaggttatta tgatgaaaag tttgttgggg
      901 aaaagatacc aacactcaaa gaaatgataa aatattctaa aggtaaggtg aaattattaa
      961 tagaaataaa acttaatgga catgaaaagg gagacatagt aaaaaaagtt gttcagttaa
     1021 ttaaagaaaa taattttgaa aatcaatgta tagttgcatc tatggataaa actatattac
     1081 aaaaagtaaa aaaactggat gagaatttac tgacatgcta ttttatggca attgcatatg
     1141 gtgatttttc aaagttaaat tatgttgata tatatggaat tgaatcaact tttgttaata
     1201 aaacagtagt agaaaagatt cataaaatga acaaaaaaat cttcgtttgg actgttaata
     1261 gtgacagttt aattgagaaa atgcttgacc taaatgtaga tagtattatt acagataatc
     1321 cataccttat agagagtgca atttattgga agaaaaatgg atttattagt caagtgtcag
     1381 attatttatt ttaaatattc tataattgta ttatccagta aaagagatat tattaaaatc
     1441 aataatatct ctttttaaca taaataaggt tgttaaatta aggttattct tataaaaatt
     1501 gtgcttatca gctataaaca aggaataaaa gtgaaaaaat tgctctgaga ttattacagt
     1561 taaaagagtt ttaagatgac agagagatag attgtcaact gtatatttaa taaatcttgt
     1621 tatttattaa attttatata agtaaaatat ataaattaat aaatcttatg ctagaatata
     1681 tatgaaaaac aatataaatt tggaggatta aaaacatgac agagcgagaa aaaatgttgg
     1741 caggagaact ttacgattgt ggtgatattg agttgttaac tcaatggcat aaagcaaaag
     1801 acctagtaag agattataac caaacagatt cctcggattt ggaaaaaaaa gaaaaaatat
     1861 taaatgaatt acttggtggt aaggataaaa acttatggat tactgcaccg tattatgtag
     1921 attatggaaa taatatttat tttggaaata attgtgaagt caatatgaat tgtacgtttt
     1981 tagatgataa taaaattatc attggggata atgcactaat cgcaccaaac gtacaaatat
     2041 atactgcatt tcatccaact aatgctggtg atagatttgg taatgtcaaa gaagatggtt
     2101 catttgaatt ttgtaaaact caaacagctc cagttactat tggagataat gtttggattg
     2161 gcgggggtgc tattatcatg ccaggtgtta ctattggaga taatgtagtt ataggtgcag
     2221 gaagtattgt tacaaaagat attccaagta atatgattgc atacggaaat ccatgccgta
     2281 ttattcgaga aaacaaataa agttaaattt tcttgcaaaa tctcaaaaaa cacctttctt
     2341 actttataca tagggtattt tccaatactt tcacatccat attcatttgt taaatttttg
     2401 tggaatgtag tttttatcac ttctatctta catttccacc attttttata aaagtcattc
     2461 ctttatagat ttatttataa tagtatatat tctatttgat gtttgaataa gcacttattt
     2521 ttaagtaaaa tactaacttt taggttagta ttttgctata gtatgatgat ta
//
LOCUS       DAEPOZ010000338         2536 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.339, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000338 DAEPOZ010000000
VERSION     DAEPOZ010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.339"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            136..1908
                     /locus_tag="KR440_003540"
     CDS             136..1908
                     /locus_tag="KR440_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0700540.1"
                     /translation="MENGKFQFFKGVRNCFNMIYLHDKRYLFISMFMAIFQGIVPSIS
                     LLVSQNIINNMQTFSKSVKEILVLLIMYLIINIIPSIFQSFYGYYRIKFNKNFEKHIE
                     TMMLEKSLTLSLKDFEDDNTYDMINRAQSQKGFNITILSENLISIIRESVSTLSLIVV
                     LSQFQLWLIIVVIVIPVLKSLYSIKLGKEQYDVSVNRTWKERKAWYIDYLLSKGYAYK
                     EIMVFRIGSKLINHYKKLKEDIISQDMNIAKKNFIATLLCTVLDSIVSAGIIGFIIIQ
                     GILKKILIGDATTYIQAVQSIKDNVESIFSLFSSVIQDLFYTDLLFKFLDYNCKEDSI
                     EHIKIFEKIGDINKIELINISYKYNHSSEYSLKNINLTLNKNDPIAILGKNGSGKTTL
                     LKILLGFYDDYEGDILINGKNLKDIEKDEYIRKVSCMFQDYIKYEATLKENVAMGDVY
                     KSNLEEKVKSKLKETKLKPSIYEEDGLETILGTWFGKKQLSMGEWQKVAISRTLMKDA
                     DMYIFDEPDSSLDSEAGNELLSLYKKTFENKIGIFITHHIQHAKKITNNIVVLENGKL
                     LESGRHDELMDKKGVYFNLFCNCK"
     gene            1930..>2536
                     /locus_tag="KR440_003541"
     CDS             1930..>2536
                     /locus_tag="KR440_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH0700541.1"
                     /translation="MNQKPFIHTFKAGKSYFIYDVNTDKILKVNAAVYNYLNKIEHNL
                     EKESDWNIEIEDEINTLINYGFLKNKRVNKTMHPETKYIKYHIENNLSSIILQVTQNC
                     NLRCDYCIYSGSYSNRVHTNKRMNIDVAKRSIDYLIKNSSQLNEVSVGFYGGEPLLEF
                     DLIKQIVEYVKVVGEGKEIYFNLTTNATLFNEEIIEFFVKNN"
BASE COUNT         1037 a          230 c          379 g          890 t
ORIGIN      
        1 tacaattcta ttaacaattt tattttaaag atttaattta aaatttaaat atgtattata
       61 cataataaaa taatgttata ttatattaat taataaaata ttttatttca attttattaa
      121 gtatggaggt gtattgtgga aaatggtaaa tttcaatttt ttaaaggtgt aagaaattgt
      181 tttaatatga tttatctaca tgataaaaga tatttattta tctctatgtt tatggcaatt
      241 tttcagggga ttgttccatc tatttctttg ttagtaagtc aaaatatcat taataatatg
      301 caaacatttt cgaaaagtgt aaaagaaata ttagttttgt taattatgta tttaatcata
      361 aatataattc cttcaatttt tcaaagtttt tatggatatt atagaataaa gtttaataaa
      421 aattttgaaa aacatattga aacaatgatg ttagagaaat ctttaacttt gagtttaaaa
      481 gattttgagg atgataatac ttatgacatg ataaatagag ctcaatctca aaaaggattt
      541 aatattacta tactatctga aaaccttatt tcaataataa gagaatcagt atctacactt
      601 tctcttattg ttgtattatc acaatttcaa ctttggctaa taatagttgt tattgttatt
      661 cctgtattga aatcattata ttccattaaa ttaggaaagg aacaatatga tgttagtgta
      721 aacagaactt ggaaagaaag aaaggcctgg tacatagatt atttgttgtc aaaaggttat
      781 gcttacaaag agattatggt atttagaatt ggttctaaac ttattaatca ttataaaaaa
      841 ttaaaagagg atattatatc tcaagatatg aatatagcta aaaagaattt tattgctacg
      901 ctattatgta cagttttaga ttcaattgta tctgcaggaa ttataggatt tataataatt
      961 caaggtattt taaagaagat tttaatagga gatgcgacta catatataca agcagttcaa
     1021 tctataaagg ataatgttga atcaatattt tcgttattta gttcagtaat acaagatctt
     1081 ttttatacag atttactgtt taaatttcta gattataact gtaaagaaga ctcaattgaa
     1141 catataaaaa tatttgaaaa aataggggat ataaataaaa tagagttaat aaatatttca
     1201 tataaataca atcattcatc agagtattct ttaaaaaata ttaatttaac tttaaataaa
     1261 aatgatccta ttgctatact aggaaaaaat ggttcaggta aaacgacact tttaaaaata
     1321 ttattaggtt tttatgatga ttatgaaggt gacatcttga taaatggaaa aaatttaaag
     1381 gatattgaaa aagatgaata cattagaaaa gtaagttgta tgtttcaaga ttatattaaa
     1441 tatgaggcta cattaaaaga aaatgtcgcc atgggagatg tatataagag taacttggaa
     1501 gaaaaagtta aaagtaaact taaagaaact aaattgaaac caagtattta tgaggaagat
     1561 ggattagaaa ctattttagg aacctggttt ggtaagaagc aactttctat gggtgaatgg
     1621 caaaaagtag caatatctag aactcttatg aaagatgctg atatgtacat ttttgatgaa
     1681 ccagattcat ctttagattc tgaagcggga aatgaactcc tttctttata taaaaaaaca
     1741 tttgaaaata agattggaat atttataacg caccatatcc aacatgcaaa aaagataact
     1801 aacaatattg tagttcttga aaatggaaag ttgttagaaa gtggaagaca tgatgaactt
     1861 atggacaaaa agggtgttta ttttaattta ttttgtaact gcaaataaat attaaaggga
     1921 ggttaattaa tgaatcaaaa accatttata cacacattta aagctggaaa atcatatttt
     1981 atttatgatg ttaatacgga taagatttta aaggtaaatg ctgctgttta taattatcta
     2041 aataaaattg agcataattt agaaaaagaa agtgactgga acatagaaat agaagacgaa
     2101 attaatacct tgattaatta tggatttcta aaaaataaaa gggtaaataa aacaatgcac
     2161 cctgaaacta agtatataaa atatcatata gaaaataatc tatcctctat tattttacaa
     2221 gtaacccaaa attgtaattt acgttgtgac tattgcattt attctggttc atacagcaac
     2281 agagttcata caaataaaag aatgaatata gatgtagcaa aaagaagtat tgactatttg
     2341 attaagaatt ctagtcaatt aaatgaagtt agtgttggct tttatggagg agaaccctta
     2401 cttgagtttg atttgattaa gcagatagta gaatatgtaa aagtagttgg agaaggaaag
     2461 gaaatatact ttaatctaac tacaaatgca actttattta atgaggaaat aatagaattc
     2521 tttgtaaaaa ataata
//
LOCUS       DAEPOZ010000339         2505 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.340, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000339 DAEPOZ010000000
VERSION     DAEPOZ010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.340"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            159..401
                     /locus_tag="KR440_003542"
     CDS             159..401
                     /locus_tag="KR440_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBH0700542.1"
                     /translation="MQNSMKQEPCDFSHGRFRFKTSDNRVLIPVVIFLGSIVTALCDL
                     ISRVILSPVELPISTITSLLGAPIVVMLLVKRKDRA"
     gene            404..1600
                     /locus_tag="KR440_003543"
     CDS             404..1600
                     /locus_tag="KR440_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0700543.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(1720..2445)
                     /locus_tag="KR440_003544"
     CDS             complement(1720..2445)
                     /locus_tag="KR440_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBH0700544.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGANFRFCDLS
                     NSDIRGANLENSNLEHATLNGVISDESTPFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSIDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
BASE COUNT          956 a          293 c          404 g          852 t
ORIGIN      
        1 tcctagttgt aatgaaactc acaatagaga tataaatgca agtataaata tattaaaaga
       61 aggattaaga ctaataacaa ttcaaaataa ataacttatg taagaaccgt aggaactatg
      121 gggatggcct gtggagaatt agtaagacat attttagtat gcaaaattct atgaagcagg
      181 aaccctgtga ctttagtcat gggaggttca gatttaaaac atcagataat agagttttga
      241 taccagtagt aatattttta ggcagtatag ttacagctct ttgtgattta atatcaagag
      301 taattttatc accagtagaa ttaccaataa gcacgataac atctttatta ggagctccaa
      361 ttgtagtaat gttattggtg aaaaggaagg atagggctta gacatgttga aaactaataa
      421 tttaagtgta ggatacaata acaaggtagt tataagtaat ataaatgtag aagtaaaaaa
      481 tggagaaata ctatgtcttt tgggaagtaa tggagctgga aaaactaccc tattaaggtc
      541 cttatctaaa cttatatcac caataaaagg tgaaatatat ttgaatggtg taaacataaa
      601 ttgtatatca agaaaagcat tatcaaaaaa aatggcttta gtacttacaa atagattatt
      661 aggtgattta atgactgtac aagatatagt caatatagga aggtatccat atacaggatt
      721 ttttgggagt ctatctaaga aagatttaat aatggtagat gaagcattgg agtctgtaga
      781 tgccttacat ttaaagaaaa gatattttga tgaactaagt gatggagaaa aacaaaaggt
      841 gttggttgca agagctcttg ttcaagaacc agaaataatt atattagatg agcctacaac
      901 tcacttggat ataaaacata gattggagct cataaacata ctgaaaaaat taagtaaaga
      961 aaagtcaata tcagtaatac tttcattgca tgaaatagac atagcattaa aaagttgtga
     1021 taaagttgct cttataaaaa ataataaagt catagcttat ggtcagcctg aagatgtagt
     1081 agatgaggat attataaatt cgctttatga attggatgat aaaaacttta atagtctttt
     1141 aggttcagta gaaattagta ataaatctaa aaatgaagtt tttataattg gtggtggggg
     1201 taaagcaaca ccaatttata gagcttttac taaaaaaggt ataggtctgt attctggaat
     1261 aatacatgaa aatgatattg attatgaaat tggtagaact atggggataa agatgtttac
     1321 agaaaatcca tttgagccga taagtgatga gagtttcgat ttggctatta gaaatttaaa
     1381 tgattccaag attataatag atacaggatt tagtgtagga gagacaaata agaggaatat
     1441 agatattata aaagaggctt taaaactgga taaaaaagtt tattctttta gaaatagaga
     1501 tgagagtaag aagtattatg acagtttgga tagtaagata gaacatatag ataaagtttc
     1561 tcaaataatc aatagtgcag atataaataa tttattataa tttatgaaat aaaatgtact
     1621 tatgtgctat agaaaaaaga gtatagatta taattttttt gaaattacaa tctatactct
     1681 tttgttattt tgtttatata tttataaaac tttagcattt tatagataac caatagcttc
     1741 ctctctagtt aaaaaaaagt gaatccctgt agtcgattct ttccatctat tttcattaaa
     1801 atcttcagct acaatcgttt ctcctactct atatataaaa ttttcatcag catatgcacg
     1861 agcactttta aaagattctt taccatctat actagtaatt gacagtactt ttgccttatc
     1921 acatctacaa gcatttgatg tagcagacac tctttttgcg tcgctgggta ttaataattg
     1981 taccattctg aaattaaaac attttttata gcctataaat gctcctgttt caggacaata
     2041 caatttaaaa aaaggggtac tttcatctga tattactcca tttaaagtag catgttctaa
     2101 attggaattt tctaaattag ctcctctaat atctgagtta gataagtcac aaaatctaaa
     2161 atttgcacca cttaagtctg cattttttaa tatagaattt tttaatgaac aatccgaaag
     2221 ataagaatta ttaaaaatag agttttctaa atctgctttt tctaaattta aattgataag
     2281 gtcagaccac gaaaagtcaa tattacttaa gcagtatcct cttaagtcca tattttctaa
     2341 tcttaattct tttaaatctg ccttttctcc agcattattg tttttaagcc ataaataatg
     2401 tttctcaagt gttttttcta attcttgttt agaaatagtt ttcattaaaa tcaccttcat
     2461 aaatttattt ttatatatgt agtagcaaaa tagtcatata tttaa
//
LOCUS       DAEPOZ010000340         2504 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.341, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000340 DAEPOZ010000000
VERSION     DAEPOZ010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.341"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..369)
                     /gene="mobB"
                     /locus_tag="KR440_003545"
     CDS             complement(<1..369)
                     /gene="mobB"
                     /locus_tag="KR440_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBH0700545.1"
                     /translation="MKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGYRVGVIKH
                     TCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQLYEDMDLI
                     IIEGYKNYRFKRLEVTRKGKY"
     gene            complement(374..1555)
                     /locus_tag="KR440_003546"
     CDS             complement(374..1555)
                     /locus_tag="KR440_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBH0700546.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGEVFNKVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVIGDMIILNVKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGILGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGIHHIKAIFKGEFNKLSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVEIVI
                     R"
     gene            complement(1766..2500)
                     /locus_tag="KR440_003547"
     CDS             complement(1766..2500)
                     /locus_tag="KR440_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700547.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSVVIEKSDHEKSAVSLENFR
                     RIDLGEYVGVEESRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
BASE COUNT          773 a          462 c          208 g         1061 t
ORIGIN      
        1 atacttacct tttctagtta cttccaatct tttaaatcta taatttttat atccttctat
       61 gataatcaag tccatatctt catataattg aatcatttta tataatggat tcttctcttt
      121 tagttcttca atatatgcta ctcgtttatc tgatattata cagacttttc tcgccccaga
      181 ttttctatgt ttataagtat cttttccttc ctcatccata tcaaaatcat ggcaggtatg
      241 tttaataact ccaacacgat accctttttt atttaattct tttattatgc cttcaatcaa
      301 cgtagtttta cctactcctg aatatgagac aactgacaat atattataca taaaattgtg
      361 tcctttcata aaactatcta ataacaatct ccactaattg attttctttt attgtctcat
      421 tctttttaat cttaatcata caattacttc ttagagttgc agataatcta gcttttgcat
      481 ctttcttatc atttagctct acatacaatt taccttcact tgtatatagt tttccactta
      541 caaatctatc tttatttgaa agtttattaa attctccttt aaatatagct tttatatgat
      601 gtattccctt cttatatgtt aaatactcaa taacaggaac tatcacatta tcaaatacca
      661 ctattgctgc atgtggatta ccagaaagac caaataatag cttttctccc aatataccta
      721 cataacaagc tccaccaggt ttaatgttta tcttccaaaa taattctgtt gcccctattt
      781 ttttgaatac atctggaatt aaatctttat ctccaacaga aacaccacct gttgtaatta
      841 aaaaatctac actctgtaac atattcttta cctttttttc taactccaat atatcatcag
      901 aacaattgca tattgtatct gcctgtattc caagttttaa taatcttgaa tataaggtat
      961 atctattact atcgtatatt ttaccattct ctaaagtctc atttatatct tgtagttctg
     1021 tacctgtatt caaaattcca acttttaaat catcataaac agttactttg gatattccaa
     1081 gacttgctaa tactccaata ccatcatatc ctatattctg atttttatta ataatcaatt
     1141 gccccttttt tatatcctca cccttaaata tgtaattatc gtattccttt aattttacat
     1201 ttaaaattat catatcacct ataaccgtta catcttcttt ttttattaca cagtttgcac
     1261 cctgtggtat ctttccacca gtcattatct tcactgcttc atttttattt aataccttat
     1321 taaatacttc tcctgcatac aaagtcccta taacctttaa atttctattt tcagaatttg
     1381 cattatatgc ataaccatca tagggtgacc tatcaaatgg aggactatca attgttgaat
     1441 atatatcttc tgcaactact ttatttactg catctataag atttactatc ttgaccctgt
     1501 ttattggtaa aacatttttt tcaataattt ttatggcatc ttctaattct atcattttaa
     1561 atactcccca atatattttt tatttaagat atttctatgt aatatattat agattataaa
     1621 agttggaaat acttttatct tatatttaat tataactaat agttatctat aagtgcagtt
     1681 caaattttat aaatctaata aaaaaactgt tcccagctag aaatttcctt ctatcttgaa
     1741 acagcatcat atgctttaga atattttatt ctaataaagt tccttttatt atttttacaa
     1801 ttttatcact ttcaatagtt tttatagatt ttattttgtt atccacaata cttattgtaa
     1861 gtacctcttt ttttaatatt ttgttatctt ctttcaatct ataactctta actactttac
     1921 taccttcatg attatatgct gttaaatcat aattcgcatc tcctacaatt tcatttattc
     1981 tagactcttc aacaccaaca tactctccca ggtcaatacg tctaaaattt tctagtgata
     2041 cagccgattt ttcatggtca cttttttcaa tcacaacact agattcagca tcaatgactg
     2101 atataccttg tgatgagaaa gaattggttt caacattgac aactttattt tcgttaatat
     2161 caacatataa agcacttcca tctagttcat catttccagt atattttgga taaactaatg
     2221 tcatataact tgatgtattt atatcttgca ttgtattcga ttcttttaaa actttaggtt
     2281 ttatataata aaatgtatta taaggtatac ccatattctt tataatgtac ttataatctt
     2341 tattcataat ttcattaaca tcattttgaa cttttgtcat agttttttgc tgtgttgctt
     2401 tacttgctac ttgatttttc tctttagtat tttctttatg acatccaaca agtataatac
     2461 ctaaaatagt tacacaaact aatgaccttt ttaacttcat aaat
//
LOCUS       DAEPOZ010000341         2504 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.342, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000341 DAEPOZ010000000
VERSION     DAEPOZ010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.342"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            136..1848
                     /gene="ptsP"
                     /locus_tag="KR440_003548"
     CDS             136..1848
                     /gene="ptsP"
                     /locus_tag="KR440_003548"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBH0700548.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASIHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
BASE COUNT         1003 a          239 c          460 g          802 t
ORIGIN      
        1 gagtaaaaat acagtaacaa acagtttata aaatttaata aaaaattata agtaaataaa
       61 actataaaaa tgtaaattat ttatataaat ttatagagtt tatgtgctag atgatatttt
      121 ttaggaggta gggttatggc ttacaaagga ataggtgctt ctcctggtgt tgctttaggg
      181 aaagcgttag tagttgaaca tagtgaatta gtaattgaaa aaaaatctat agataatgtt
      241 gaagctgaaa ttgctaaatt agaagatgca gttgcagttt ctaaagaaga attagtaaaa
      301 gttaaggaaa aggcttctga agaattagga gcagaggaag cagaaatatt tgaagcacat
      361 ttattagttt tagaagaccc agagttaatt ggttctgcaa tagataaaat taaaactgaa
      421 agtgtaaatg cagagtatgc tttaaatgaa ataaaagaga tgtttgttag catgtttgag
      481 tctatggaca atgagtacat gaaagaaaga gcggcagata taaaagatgt tacaaataga
      541 atacttagac atatattagg aattaaagtt gttgatttat ctgctttaag cgaagaagtt
      601 gtattaatag cacatgattt aactccatca gacacagcaa ctatgaataa aaaaatggtt
      661 ttaggatttt taactgatat aggtggaaga acttctcata ctgctatcat gtctagaact
      721 ttagaaatag ctgcaatcgt tggattaaat gatataactt caaaagttaa ggatggagat
      781 tttgtagttt tcaatggaga cacaggtgaa gtaattgtga atcctgatga agagactata
      841 aataaatata ctgaattaaa agctaagtat gaagatgaga gaaaagcatt acagctatta
      901 aaaggaaaac catctgtaac tttagatgga aaacatgtgg aattagctgg taacatagga
      961 acacctaacg atatagaagg tctaataaag aatgatgctg aaggtgttgg tctttataga
     1021 acagaatttt tatacatgga tagagattct ttcccaacag aagaaataca atatgaagca
     1081 tacaaagctg tattagaagg aatggatgga aagccgatag ttataagaac attggatata
     1141 ggtggagata aagaattaag ttatttaagt atggaacctg aaatgaatcc attcttaggt
     1201 tacagagcaa taagactttg cctagataga aaagatatat ttaagactca attgagagct
     1261 ttatatagag ctagtattca tggaagatta agaatcatgt tcccaatgat ttcatcttta
     1321 gaagaattgt tacaagctaa agaagttgta aaagaagttt tagcagaact tgattctgaa
     1381 ggtgtggctt atgctaaaga tgtagaaatt ggtatgatga tagaagttcc atctgctgct
     1441 gtaatatctg atgtattggc taaacacgta gatttcttct ctataggaac taatgattta
     1501 attcaataca cttgtgcagt tgatagaatg aatcaaaaga taagttatct ttacaatcaa
     1561 tttaatccag ctgttttaag acttataaag actgttatag acaatgcaca taaagaagga
     1621 aaatgggcag gtatgtgtgg agaatctgcc ggagaccaaa aaatgatacc aatactttta
     1681 ggtatgggat tagacgagtt ctctatgtct ccaatatcaa tacttcctgc tagaaagtta
     1741 ataacttcag ttaaagagtc agatatgaaa aaattagctg atgatgtact taatatgggt
     1801 actgctgaag aaataaaaag ctatatagaa aagactttta atatataatt aatgttatat
     1861 agttactaaa attcattttt ctaaataaca aaattatatt ttacgaatta aaaaggagat
     1921 gtttcaaaat aaaaatttga agcatctctt ttttataaaa gattactaag aattacttaa
     1981 tctttgtaat agtcattttg tgaaaaagca aaactgcatt gtgactatag tatatatgga
     2041 tgttattatc aataaaagta ctattaaaat gaagattcat tctcaaatga tatcaaaagt
     2101 agttgtgatg gagtaagata atattttgtg atttaatagt aaaaattaaa aaaatatggt
     2161 attgaaaaat aattaaaagt atttaataat agaattaggg aagaataagt ttataagtaa
     2221 aaataattga ttactagtta ataaaataaa tgtgatatcc aaataaatcc taaatctaat
     2281 ttgagtttta atatagatag tctaaagttt gattattaaa gatttaattg taagttgtta
     2341 cagttgatga tattgattag agattaaagt ttagagtaga aagttacctt aaatttaaaa
     2401 atgcaagata acccaatcta aaaattataa tcactcgata ggtttaagga tttatttgtt
     2461 tattataaat aggagggtgt ctcaaaatga actttttagt ttat
//
LOCUS       DAEPOZ010000342         2494 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.343, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000342 DAEPOZ010000000
VERSION     DAEPOZ010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.343"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..1172
                     /locus_tag="KR440_003549"
     CDS             <1..1172
                     /locus_tag="KR440_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700549.1"
                     /translation="VIDIKEPFFKALFKSLNIFEDQSYAYYSDFVGKVNNSAYVIDKI
                     IPTLFAITLIIVAYLILNDIRKKQIKSLIVIVVYSILFIGLNYISIDMSTYRDEERSY
                     GEYLKKSNISDIKIKSHDMKIDFKSKSNIKDRIVLLNTSNEELTNINMILDEIFDIKY
                     IKVNSENIKFNKKKDLIEVNLKESLEPNDEVTLDIEYEGYINVLTSWNQNKYIATKDD
                     IMLPPGSLAWYPKVNQSSCMAYKINLSSKNNVYSNLPLLNKTSNFLKKDYIFEGKSND
                     IAIYAGEFKEEVKDDIKIIYPSDSNVNMDIEEKIKFLIETRNEILGDSKASVDAKKYV
                     DEVDNVMESIKRKKINMILVADLPSNEFLYEDSKGEKISITIYHWLSGNILIIDK"
     gene            1187..2059
                     /locus_tag="KR440_003550"
     CDS             1187..2059
                     /locus_tag="KR440_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0700550.1"
                     /translation="MINKLEIRNLNFNYKNKKALDNINLTLTDGVVALLGPNGAGKST
                     LMRLLVTLYETSIGEIELNNIKYSKNNEKIKANVGYIPQDFDMYNNINGQEYLEFVAK
                     MRGVSKNDLKKHIQKVVSKVNLDKFINNKIGTYSGGVKRRLGIAQALIGDSKLIVMDE
                     PTVGLDPEQRNEFRRLLPEISKNSIVLISTHIVEDIQFNCDKLIILNQGKILYDGTIN
                     KFIDMVEVYSVVVSNDEFEYLERNIDIIDYRRVKDGVQVKYIGKNTEKLKDSLRIDTT
                     LQDAYINFLHKELK"
     gene            2060..>2494
                     /locus_tag="KR440_003551"
     CDS             2060..>2494
                     /locus_tag="KR440_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700551.1"
                     /translation="MCILELIKFELKKIELKHVLIFVIISILCIFITGILDYNELIFY
                     NDVPIMDTYSIAGWLGIIKISDVFIPIMSAITIIYVFSKDYLGNINEILTLYNKKKYN
                     QFIVFRWCVILAIYTVLLLISITIVYTFTSVKGFNIDKIDFVY"
BASE COUNT         1056 a          206 c          397 g          835 t
ORIGIN      
        1 gagttattga tattaaagaa ccatttttta aagcattatt taaatcactt aatatatttg
       61 aagatcaatc atatgcttac tattctgatt ttgtagggaa agttaataat tcagcatatg
      121 ttatagataa gattatacct acactatttg caattacttt aataatagta gcatatctga
      181 tattaaatga tattagaaag aaacagatta aatcattaat agttatagta gtgtacagta
      241 ttttatttat tggattaaat tatatatcaa tagatatgtc aacatataga gatgaggaac
      301 gtagttatgg agagtattta aagaaatcta atattagtga tattaaaata aaatcgcatg
      361 atatgaagat tgactttaaa agcaagtcaa atataaaaga tagaattgtt ttattaaaca
      421 catcaaatga agagttaaca aatattaata tgattttaga tgaaatattt gatataaaat
      481 atataaaagt aaattcggaa aatataaaat ttaacaaaaa aaaggattta attgaagtta
      541 atttaaagga aagtttagag cctaatgacg aggtgacatt agatatagag tatgaaggct
      601 atattaatgt gttgacatct tggaatcaaa ataaatatat agctactaaa gatgatatta
      661 tgcttccacc aggttcttta gcgtggtacc caaaagtaaa tcaatcaagt tgtatggctt
      721 ataaaataaa tttaagttct aaaaataatg tctattcaaa tttaccttta ctgaataaga
      781 ctagtaattt tttaaaaaaa gattatatat ttgaaggaaa gtcaaatgat attgcaatat
      841 atgcaggtga gtttaaagaa gaggtaaaag atgatataaa aataatctat ccatcagata
      901 gtaatgtaaa tatggatata gaggaaaaaa ttaaattttt aattgaaaca agaaatgaaa
      961 tattaggtga tagcaaagca tctgtagatg ctaaaaaata tgtagatgaa gtagataatg
     1021 ttatggaatc aataaaaaga aaaaaaatta atatgatatt agttgctgac ttacctagca
     1081 atgaattttt atatgaagac tcaaagggag agaaaattag cataactatt tatcattggc
     1141 tttcaggaaa tatccttata atagataaat aataaaggag gttaatatga taaacaagtt
     1201 agaaataaga aatctaaatt ttaattacaa aaataaaaaa gctctggata atataaattt
     1261 aaccctaaca gatggagtag ttgcattatt aggtcctaat ggtgctggaa aatcaacatt
     1321 aatgaggtta ttagtgactc tttatgaaac ttcaattgga gagatagaat taaataatat
     1381 aaagtatagc aaaaacaatg agaaaatcaa ggcaaatgta ggatatatac ctcaagattt
     1441 tgatatgtat aataatataa atgggcaaga atacttagag tttgtagcta aaatgagagg
     1501 tgtatctaag aatgatttaa agaaacatat acaaaaagta gtatcaaaag ttaatctgga
     1561 caagtttata aataataaaa taggtacata ctcaggtggt gtaaagagac gactaggtat
     1621 agcacaggct ttaataggtg attctaagtt aatagttatg gatgaaccta cagttggtct
     1681 tgatccagag caaaggaatg aatttagaag attgttacca gaaataagta aaaattctat
     1741 agtattaatt tcaactcaca tagtagaaga catacaattt aactgtgaca aattaattat
     1801 attaaatcaa ggcaaaatat tatatgatgg tacaattaat aaatttattg atatggttga
     1861 ggtgtattca gtagtagttt ctaatgatga atttgaatat ttagaacgta atattgatat
     1921 tattgattat agaagagtta aagatggtgt gcaagtaaag tatataggta aaaatacaga
     1981 gaaacttaag gatagcttaa ggatagatac aactttacaa gatgcttata taaacttttt
     2041 acataaggag ttaaaataaa tgtgtatatt ggagttaata aaatttgagc ttaaaaaaat
     2101 agagcttaaa catgtgttaa tatttgtaat tatttctata ttatgcatat ttattacagg
     2161 catattagat tataatgagt taatatttta taatgatgta cctataatgg atacatattc
     2221 tatagcagga tggttaggaa ttataaagat atcagatgtt tttataccaa taatgtcagc
     2281 aatcacaata atttatgtgt tttcaaaaga ttatttgggt aatataaatg agatacttac
     2341 tttatataat aaaaaaaaat ataatcaatt tatagtattt agatggtgtg ttatattagc
     2401 tatatatact gtattattat taatttcaat tacaatagta tatacattta cgtcagtgaa
     2461 aggttttaac atagacaaaa tagattttgt ttat
//
LOCUS       DAEPOZ010000343         2458 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.344, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000343 DAEPOZ010000000
VERSION     DAEPOZ010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.344"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            149..769
                     /locus_tag="KR440_003552"
     CDS             149..769
                     /locus_tag="KR440_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBH0700552.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(935..1471)
                     /locus_tag="KR440_003553"
     CDS             complement(935..1471)
                     /locus_tag="KR440_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBH0700553.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(1584..2258)
                     /locus_tag="KR440_003554"
     CDS             complement(1584..2258)
                     /locus_tag="KR440_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBH0700554.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
BASE COUNT          932 a          291 c          338 g          897 t
ORIGIN      
        1 tataatatat aataaaataa ttatacctta actgtaaact tatatagatt atttactttt
       61 taatattaga ttcatttttt gagattggta tataataaac atatagagta tataataata
      121 gttaaagtta aaaaagggga aaagcaatat gcagtatata caagaattac ttgatttgac
      181 tcaaaattat tgggtgttag ccacaataat cggcttatta agtgcattta tagaaagctt
      241 tataccagcc ttaccacttg ttgctatagt tacagcaaat gctgcaatac aaggattatt
      301 tattggatgt ttgttatcat ggattggctc aggacttggg acaacttcat tattcttatt
      361 aataagcaga tttaatgata gtaagctttt taataagttg agaaatagta aaactgaaaa
      421 agcaataagt tggatggata aacagggatt taagttatta tttatagcgt atgcctgtcc
      481 atttatgcct ggttgtttag ttactatcgc atcagctttt ggtaaaaaag atttaaaaga
      541 ttttgtgcca gcaatgttgg caggaaaatt tgtaatgttt atagttataa gttacgtggc
      601 aagtgatata gaaggtttta taacaagtcc tttaaagata gcatcattta tattattggt
      661 gtttttgtca tggaagattg gaaatagagt aaataaaaat cttgaaaatc ataattatga
      721 tttccatcat aaaaaacatc acaatgataa agatgataaa atgatttagc ttgtacgaaa
      781 taaaacgtat cttaaaatga tttagtacta aattatttta agatacgttt tattttttgt
      841 attaaggtac aaaattttta gttcttttgt aaactaaaat atataaaact tatttatact
      901 tagttcaaaa tattgttatt tattttttct acaactataa taaaatataa aattgatatc
      961 caatcaaaaa aataactatg aaatttaata gcttagatat gaatttttct gtttttgagt
     1021 tagtttttat aggtagtaaa gaaatgttta tggtaatggg ataaaacaat tcaatacctt
     1081 ctttagtaat aaggtcaaga cacaagtgag atagatatcc tattataaac ccactggaca
     1141 aacatgagaa aactatatta ttgttagtga agattgtcag agatttaaaa aaactataaa
     1201 taagtaataa ggcaattaga ctgtgagtaa accctctatg tctaaaccta cttccaaata
     1261 atttgtatat taacataaat tttttactta tgtaagaacc tctcatatct atatctggaa
     1321 ataaagaacc taaatatgca aaataaatat aaattataat taatactatt ttatatgcaa
     1381 tatcgtattt aactaggtac agattgttta taaaggggag tatcaaaagt gcgaatatat
     1441 aacctcccct ttcatgtgtt tcttttatca tataaaaatc ctcctgaatc gtcactataa
     1501 acattatatt ggtaaattta gactatcttt tattatatta tatagcaaac ataagttttt
     1561 ttcaaaagga ttttaatatt taattaatct tgagatttta aatttttgta attttctaaa
     1621 gcaccattcc aatttactac attaaaccag ttgtcaatgt attcatttct tctattttga
     1681 tattttaaat agtaagcatg ctcccaaaca tcaagtccta ttataggagt taggttttta
     1741 cttacagggc tatcctgatt tggagtagtc ataatagata atttcccatc tttagtagct
     1801 acaagccaag cccaaccaga accaaagaca tctaaagcag atttttggaa ctcttgctta
     1861 aatttttcaa aagaaccaaa gtctctatca atagcttctt ttaaagattc agaaggtatg
     1921 gttttttctg gcgtcattat atcaaaaaag aatttatgat tataagctcc acctgcatta
     1981 tttcttacag ttgtagcaat atctttaggt aaagaatcta aattttgcaa taattcacat
     2041 aaagaataat tataaagctc aggatatttt tcaagagcag catttaattt atcaacataa
     2101 gcttgataat gcttatcatg atgcagtttc attgtttctt tatctatata aggttcaagt
     2161 gcatcatatg cataaggtaa tggtttaacc ttaaatttgt tattttcagg tgtaaaagca
     2221 aatgaagtta tgcactgtga gattataaat aaagacataa taactggtat taatattttt
     2281 ttcttcataa aaatcacctc ctaatatagt atttctagaa ataaaaaaaa tatgaaatat
     2341 taacagataa tttataagaa tttaatagca atttcatttt aaattgaata agttttaaag
     2401 ccaaagacaa aactatgaaa tatagaataa gactaaagga cagaaaggaa ttttgatt
//
LOCUS       DAEPOZ010000344         2453 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.345, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000344 DAEPOZ010000000
VERSION     DAEPOZ010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.345"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            113..1015
                     /locus_tag="KR440_003555"
     CDS             113..1015
                     /locus_tag="KR440_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBH0700555.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIATF
                     GKLLIPFIMAGILGIVMPDVLGSGHNLVEELTSHEYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTSHIF"
     gene            1624..>2453
                     /locus_tag="KR440_003556"
     CDS             1624..>2453
                     /locus_tag="KR440_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBH0700556.1"
                     /translation="MVSSFLNDIFRTILVSCIFIVILLVFRITLFKTFSKKFNYYIWL
                     IVIIKLSLPFMNYTFIFNESKYNGNINKISLGNFNSISIVYSTVLVSAWIAITIFYLV
                     YTLLKYIKLKNMINDLSYDVEDDKIKNLYENLLKELKINKKIRLKYSYEVESPVFFNS
                     CVILPPCDYTLKELNWIFRHELMHFKSKDLYIKYLVIFLKCVYWFNPFVYIMEKFIDL
                     DCEFYCDERVLKNCTIEEKQDYALTIISAMRKGSNSSNKFVAGLHKESDIKKRVFSMF
                     "
BASE COUNT          822 a          225 c          480 g          926 t
ORIGIN      
        1 gtaggaacta cggggatagc ctgtggagaa ttagtaagac atattttagt atgcaaaatt
       61 ctatgaagca ggaaccctgt gactttagtc atgggaggtt cagattggaa atgtgttgcc
      121 acaagattat tatggattaa tagtgatttt aggtgttatt cttggagtta tgggggcatt
      181 ttacaactgg tttactctat atgtgcagtc tttatacaag aagattccta aaattgctac
      241 ctttggaaaa ctgttgattc catttataat ggctggtatt cttgggattg ttatgcctga
      301 tgttctgggg agtggacaca atttagttga agaacttaca agtcatgaat atatttttgg
      361 aatggttgtt ttgatttttg ttatacgttt tatattttcg gctgttagtt ttgggtcagg
      421 tgcaccaggg ggaatatttt ttccattgct tgttttagga gcatttatag gtggaatatt
      481 tggtatggta ggtgttaaag tttttggaat gaatcccgat tatgtaaata actttgtgct
      541 tttggctatg gcaggatatt ttacagctat tgtgcgtgcc cctttgactg gtatcatatt
      601 aatttttgaa atgacaggtt ctgttagtca aatgctttca ctatcagtaa tttcaattgt
      661 ggcatatatt gtggctacac ttatgaagtc taaacctatt tatgaaagtc ttcttgagag
      721 gttacttgaa aaaaatggag aacctgtaca taaagagagg ggcgaaaaga tactagacca
      781 gttctctgtt atgtttggct ctcctgttgc gaacaaggca attcaggatg tacagtggcc
      841 tgaaaattgt cttttggtag caattcaaag aggtggagaa gaaattattc ctagaggtaa
      901 gacaattctt agaccaagtg atgtaattgt gactatgaca gatgagagag atagtggtgc
      961 tgtgtatgat aagatggaaa atttgtgtaa agaacatacg tcacatattt tttagggata
     1021 atgatgaatg tttaataatg atagaatatt taatgaagat ttgattaaaa aattggtaat
     1081 ttgaaagaga gttaaggaat taatagaaaa tgaaaatgtg atatcttaga tgtaaaagag
     1141 tttgaagaaa ataattgtat atatttgtta atgagattat atggactaat ggtatattta
     1201 tactgtgcta aaagttgcac tattgtatga ggaatgggaa taaagggttt gaaaaaatag
     1261 gtagaagatt tttcagatat tcttattggg atattttaat taatatattt taattgcgat
     1321 attttgggaa gaatagaatt tatttataaa tgaaaataat taaaaggggt taaggttgta
     1381 ttagcccctt ttgcttatag agtttggaaa tggtatcttg atagcagttg ttttatgctt
     1441 attatatttt attacatatt ttaaaattaa ccttatcact cctaaatcat tattctatca
     1501 acttttaaaa cactttcaag acaatcacct attgaaaaca aagttaatta aaaacctaca
     1561 tatcagttta gtattcgtgt agatgattat ataaatataa aagtataaga aaggtgagta
     1621 tctgtggtta gttcattttt aaatgatatc tttagaacaa ttttagtaag ttgtattttt
     1681 atagttattt tattagtctt tagaataact ttatttaaga ctttcagtaa aaaatttaat
     1741 tactatatat ggcttattgt gattattaag ctatcattac catttatgaa ttatactttt
     1801 atatttaatg agtcaaaata taatggtaat attaataaga taagtttagg caattttaat
     1861 agtatttcta ttgtatatag tactgtttta gtctctgctt ggattgctat tacgatattt
     1921 tatttagttt atactttatt gaaatatatt aagcttaaaa atatgataaa tgatttgtct
     1981 tatgatgttg aggatgataa gataaaaaat ctttatgaaa atttattaaa agagcttaag
     2041 ataaataaaa aaatacggct taaatattca tatgaagttg aaagtccagt attttttaat
     2101 tcgtgtgtca ttttaccacc atgtgattat acattaaaag agttaaactg gatatttagg
     2161 catgagttga tgcattttaa gagtaaagac ctttacatta agtatttggt gatattttta
     2221 aaatgtgttt attggtttaa tccttttgtg tatataatgg agaagtttat agatttggat
     2281 tgtgaatttt attgtgatga aagagttttg aagaattgta cgatagaaga gaagcaggat
     2341 tatgcattga caataattag tgcaatgaga aaaggttcaa atagttcaaa taaatttgtg
     2401 gctggattac ataaggagtc agatattaaa aaaagagtgt ttagtatgtt taa
//
LOCUS       DAEPOZ010000345         2453 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.346, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000345 DAEPOZ010000000
VERSION     DAEPOZ010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.346"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(707..922)
                     /locus_tag="KR440_003557"
     CDS             complement(707..922)
                     /locus_tag="KR440_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700557.1"
                     /translation="MHINQTGFISNGLGIKQQQKKEPTSNSFSYNAPSSWKPVGRDSV
                     QYNCGFELIATLVREGSSWAFNFIVQP"
     gene            complement(1105..1449)
                     /locus_tag="KR440_003558"
     CDS             complement(1105..1449)
                     /locus_tag="KR440_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700558.1"
                     /translation="MNYRLKKSVTTILSLLIMTILLPTNASASKVEDSSMKVMYSEKQ
                     VTDMDKLYDMAKNGITDVKSSNEKGIIKNARTGESIYVDSISTTQLLEVKQSKNLTSK
                     IYSKTVFLILIQ"
     gene            1576..1959
                     /locus_tag="KR440_003559"
     CDS             1576..1959
                     /locus_tag="KR440_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700559.1"
                     /translation="MRKKFFIGILIVLIISVIAILNIKNDKNVFIGESENWKAKFVTT
                     EGHSKLEIKYKNSKDVVEQKFNYSYQFIDGEDAVVVVKNEILEAGQEKAIKIQRPGLY
                     SNRESSYKLIISWNGNEETINLKKK"
BASE COUNT          964 a          292 c          291 g          906 t
ORIGIN      
        1 taatacaaaa atgtttaata cattagataa gaaatacttt aaatatgttt catacaagga
       61 ctcaaaagaa ttaataagtg attttaaatc tgtatatagg tcagttaatg aataaaaagc
      121 cttatcgact ttagataaaa tagattaaaa ataatgtaaa cagtatactt cagaaattaa
      181 aacgtttgga aataactgga atgttgtagc atcagccttt agttttacta aaataagtaa
      241 tatctataat atatattaca agtataatcg aaggatttaa taaatatttc aaaaatgtct
      301 caaaaacaaa gtccagattt acatcaaacg cttcactctt aaagatatta ttcttgacaa
      361 gcgaaaatgt gatgaaaaaa tggactatga aatataaaaa ttgatataat atatacaatc
      421 atttattcat atcttttgat agtagaatat aattttaaaa attataccct aaaaatttgc
      481 tataattgtt ataatatata taatttccaa aaatatttaa aaacatctca atatacattt
      541 tttataattt gttaaaataa aattcatata ttaagaaata ttaatagtaa ataagcaatt
      601 gttactataa cgtaggaatt atctttaaaa acacaaatta atttacattc tctaatttta
      661 aagtatatgt atatctttga ttttaataaa attcacttaa aatattttaa ggttgaacaa
      721 tgaaattgaa tgcccaagaa cttccttctc ttactaatgt tgcaattaat tcaaacccac
      781 aattgtattg aactgaatct ctacctactg gtttccaact agaaggtgca ttatatgaga
      841 aagaatttga tgttggctct ttcttttgtt gctgcttaat tcctagccca ttagaaataa
      901 atccagtttg atttatatgc acccttctat ttgttacacg aatctggccg tctgcaatag
      961 accagtttcc agaaacttta ctaacggaaa tatattgaaa tgctccatct ccttttgata
     1021 ttgtatatgt aactgtacca gatgctttta caccaccagt tttatcccat ccatcttctg
     1081 atatacttgc ttttctaaga ggctttattg tattagtatc aaaaaaactg tcttagaata
     1141 tatctttgat gttaaatttt tagattgttt aacttctaat aattgagttg ttgatataga
     1201 atcaacgtat atggactctc ctgttcttgc atttttgatt ataccttttt cattggaact
     1261 tttaacatca gtaattccat ttttagccat atcgtataat ttgtccatgt ctgttacttg
     1321 tttctctgaa tacattactt tcatagatga atcttctacc ttactagcac ttgcatttgt
     1381 tggtaatagt attgtcatta tgagtaaaga taaaatagtt gttacagatt tttttaacct
     1441 ataattcata ttaatcctta ctttttattt gttataacag acattctatt gtaatagaat
     1501 ataaatccac aatattttta aaattagtat tgaataacta ttctcatgtt ataattattc
     1561 aaaggaggga ttgatatgag gaaaaaattt tttataggta ttcttatagt tttaataatc
     1621 tctgtgatag ctatacttaa tataaaaaat gataaaaatg tctttattgg agaatcagaa
     1681 aattggaaag ctaaatttgt aactactgaa ggacactcaa aattagaaat aaaatataaa
     1741 aatagtaaag atgtagttga acaaaaattt aattattcct atcaatttat agatggtgaa
     1801 gatgcagttg tagttgtaaa aaatgaaatt ttagaagcag gtcaagaaaa agcaataaaa
     1861 atacaaagac caggtcttta ttcaaataga gaatctagtt acaagctaat tatatcttgg
     1921 aatggaaatg aggaaactat aaacttgaaa aagaaataac catattatca atttttaaaa
     1981 aattattatt caaattctta taaaaagagt gtttagaaaa atgaaacact cttttttgta
     2041 taagtttttg aaaactataa atgaaatata tcaaaatcgt tcgaaaaatt actgaaataa
     2101 actcttaaat atattataaa ttaaggtata ttatacatga atttattggg agtttatgag
     2161 ttttaaaatt ggaaaccaaa caataagggc taaaaatact ttaaggaaaa tatataaagt
     2221 tctataaatt atcaaaaata tttcgcaaaa attgataatt attacaaata aattgataat
     2281 actaataata ttagaaatat cgaaattgaa caaactagga ttaaagctca attagaaaaa
     2341 tccactaatc ctgagaatgc tttgttattc tcatttatta ctgttgtttt ttcattattt
     2401 gctaatttat ttttcagttc attaaatgtt aaatataact ctataaattt aat
//
LOCUS       DAEPOZ010000346         2401 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.348, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000346 DAEPOZ010000000
VERSION     DAEPOZ010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.348"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..313
                     /locus_tag="KR440_003560"
     CDS             <1..313
                     /locus_tag="KR440_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBH0700560.1"
                     /translation="VYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKITAKKMSKNIY
                     DYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIKMDNLIMDSLG
                     I"
     gene            545..1756
                     /locus_tag="KR440_003561"
     CDS             545..1756
                     /locus_tag="KR440_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBH0700561.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIIQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            1948..2208
                     /locus_tag="KR440_003562"
     CDS             1948..2208
                     /locus_tag="KR440_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBH0700562.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
BASE COUNT         1022 a          199 c          403 g          777 t
ORIGIN      
        1 tgtatattct ttttttataa aagaagagta tttagataaa ttttcatatg aatatctagt
       61 tggaatttta aattccagtg tttatgataa atactttaaa ataactgcta aaaaaatgag
      121 taaaaatata tatgattact atcctaataa ggttatgaag ataaggattt ttagggataa
      181 taattatgaa gagatagaga atttatcaaa gcaaataata tcaattttat taaataagtc
      241 tatagataaa ggaaaagtag aaaaattgca gataaaaatg gataacttga taatggattc
      301 tttaggtata taagcagttg taagtattaa aaaattataa tcctttaatt gtttatttaa
      361 atttagaaat aaaaagttag ccatataaaa aatattaaaa atatatactt ttcaatattt
      421 ttttgataaa ataagaatga atttgtgcaa ttttattttt ctccaataac taaaaatagt
      481 tttttgaatt aatatttgct taagaataaa aatcagaaac gtttttgata tagtaggtga
      541 taatatgaaa ggattagttt tagaaggtgg aggaacaaaa ggagcctacc aaataggtgc
      601 atataaagct ttaagagagc ttggtataga gtttcaagga gtaactggca catcaattgg
      661 tgctttaaat ggagcatata taattcaaaa tgatattgaa gtaatggaag atatttggtt
      721 aaattatgac tatactcatt ttatgaatat tgatagagaa acctatgaga ggtttaaaaa
      781 tgtagatttc actactaaaa atctcaatgc agtgtttgag cttataaata aagctagaaa
      841 aaatgaaggt atagatataa gcccattaag acaactaatg gagacaacat taaatgaaga
      901 cataataagg aaatcaaaaa aagactttgg acttgttacg gtctggtggg ataaaaaaat
      961 taatccatat ccattgtata tcgaagatat acctaaagga agattgattg attatttaat
     1021 tgctagttcc agtttgccta tttttcaaac gaacatgatg gatgataagt tgtatcttga
     1081 tggaatgttt tgtgataaca tgccaatagg actcttggaa gacaaaggtt atgaagatgt
     1141 tgttgtaatt aggttgattg atgattttat tgggaaaatg aatctaaatc gacatcaaaa
     1201 tataaatata aaaactataa taccatcaga atatctagga ggttctctta ataaagatag
     1261 agataatgta gaaaaaaaca tccgactagg ttacttagat actatgaaag catatgaaag
     1321 atgcactgga attaagtatt attttgatgt agagtataaa tataatgaag aatattgttt
     1381 tgataaattt agaagtatga gcaatgaaac aatacaatat atctgtaatt tattgaatat
     1441 aaaaagagat gcttcactta gaactgttat ggaaagtgta ataccaaagc ttggtgaggt
     1501 gcttggattg ccaaaggact tttcatataa agatttgttt tactgtatat atgaaaagaa
     1561 acttgaagaa aataatataa acaggataaa attatatgat tttaacaagg ttatagatgt
     1621 tgtaaataca aatattgatt ctaatagaaa tttagcttct aattttgaag ttagacctaa
     1681 aattagtatt aaaaagaaca aaaacacaaa attattgaca aattgtatta taaaagacat
     1741 aaaaaatcaa agttaaataa aaaacaaaaa aaaatttgta attatactcg aaaaaagtgt
     1801 atattagtag tataatattt ttgtagcact agtgggaaca aaaaatgacc ataggacaaa
     1861 aaagtaccac ttagtaccat taaagtaaaa aacaggaaaa caattttaaa aaatataaaa
     1921 tgtaaagttt ataggaggat ttaaaaaatg gaaaaagtag ttagtatcaa aaatgcaagt
     1981 ggattacatg ctagaccagc aggaatgttt gttaagaagg cagctgaatt taaatcaaca
     2041 gtagaggttg tagcaaaaga caaaactgta aatgcaaaat caataatggg aataatgagt
     2101 ttaggtttag ctcaaggaga agaattaaag ttagttgtaa atggagaaga tgaagaagta
     2161 gctttagctg caatggttga attagttgaa agtggatttg gagaatagat ataataattt
     2221 tttaaaattt gatatttaga atctaatatt acttaaagta aagtaacaga gaagtattaa
     2281 acatatacag accaacattt gtagatattt aatttctttg ttactttcat gtttatttaa
     2341 tcagaagatt aagactattg ttaattgggt ataaaaatat aaatatttta gaaaatgaaa
     2401 t
//
LOCUS       DAEPOZ010000347         2396 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.349, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000347 DAEPOZ010000000
VERSION     DAEPOZ010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.349"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(265..1116)
                     /gene="gatY"
                     /locus_tag="KR440_003563"
     CDS             complement(265..1116)
                     /gene="gatY"
                     /locus_tag="KR440_003563"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBH0700563.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVKIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            complement(1165..2334)
                     /locus_tag="KR440_003564"
     CDS             complement(1165..2334)
                     /locus_tag="KR440_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBH0700564.1"
                     /translation="MKMFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKL
                     NRFIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNP
                     YDYLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPQGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPK
                     SFIDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFK
                     EKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
BASE COUNT          742 a          437 c          249 g          968 t
ORIGIN      
        1 taaaaacaac cttttctttt tatttaagaa ttttatatcc tagtatattt tgattccaaa
       61 atttaatata catattttat aataataaaa tatatagttc aatttttatt atattttaat
      121 tatatatgta atactttacc aataaaagct gatatttttc atcaattaaa tgaaaaaaat
      181 acaccattta catataatta atataaattt ttcatataac aaatatccta aacttatcta
      241 aaataaattt aggatatttt atttttatac tctatttaca gacccacaca ctttaatctt
      301 ttcttttaca aaatccttca ctgattgaag ccctgctaat ccatactttt tagggtctat
      361 aacctcagaa tcttcatcta aaactttttt aaccccttta ctatatgcaa ttcttaattc
      421 agttgcatag ttaacttttg caatacctct ttttatagct tcttttataa tatcatcatg
      481 tattcctgaa cctccatgca atactaaagg tattgaaact acatttttaa tttctgataa
      541 cctatctaaa tctattttag gttcaccctt atacaatcca tgtgcagtac ctatagcaat
      601 agccaatgaa gatattcctg ttctatcgac aaactctttt gcctcttttg gatttgtata
      661 atcattacta ttcccgccat ctaaatcatc ttcttttcca ccaactttcc caagttctgc
      721 ttccactggt ataagagaag gactacatgc ttttactact gatttagata tagatatatt
      781 attttcaaag ctttcatgag aaccatctat cattattgat gtatatccac ttcttaaagc
      841 tttcattgca aggtcaaaac ttgaaccatg gtccagatgc attgctacag gtatctttac
      901 tctttctgca gcaacctttg catttgctag ataataatca agacctgcat attttacagt
      961 agatggagta gtttgtaata ttactggaga gtttaactcc tcagctgctt ctataacagc
     1021 aattaccatt tccatatttt ctacattaaa tgctcctact gcatacccct catcttgtgc
     1081 ctttttaagc attgatgttg ttgttactaa tgccacattg ataccctccc aaattattta
     1141 gttataatat tttaatctta tcttttaata ttcatatatt gttacacctt ttactactct
     1201 atttactgtt cctgtagctg atggcgtatc tggcttattt ccaaccttta ccgatacaaa
     1261 caatgctata gtttgtgcaa acaatatgta tggcattgca agatacacat ctggtaattt
     1321 attatacttt tctttaaact caaactttgt tcctgagaag ttattttcat tttctactga
     1381 cactgcacaa gtgaatttag caattttatc cctttttatc tcctctaaaa catctaaatc
     1441 atattttctt gtatataaac agttagatac aaattcaaat acaagtgtat tttcatcaat
     1501 aaatgatttt ggtccgtgtc taaaccccat aggagaatca tacaccgtag atatttttcc
     1561 tgctgtaagt tctaaaagtt ttagttgtgc ttcttgtgtc aaaccaccta atcctcctga
     1621 acctaaatat acaactctat cgaaatcttt atttattaac tcatgtattt catcttttct
     1681 atctataaca tttcttccca tttcaattat tgcatttata tatgactttt ctacatcatc
     1741 ttctaaacta tcgaatatta acatagcaga taacatcata cacgaaaaac ttcctgtcat
     1801 agcaaagcct tcatcatttg attttgatgg cattaataac aagtaattat tttcgtcgtt
     1861 tttagtcatt ttagctagtt ctccttgcgg attacaagtt atagctaaat gataaaaatt
     1921 atctacaatc ttattcccta aatttaaagc agccaaactt tcaggactat ttccacttct
     1981 tgcaaatgaa attaataatg ttggtatatc tttttttaag taatcatatg ggtttgaaac
     2041 tatatccgta gtaggtattg cttcaaatat gtattttctt atgtcatttt tattcttaag
     2101 gtaaggaagt attgaatttc ctatgtaagc tgaagttcca gcacctgtaa atataactct
     2161 aaactgccca tgattattac ttattgtatc tatgaatcta tttaattttt ctttgttaga
     2221 tttatatatt tcataagtct ctaaccataa ctcaggttgt tgttttattt cattagttgt
     2281 tattattgca cctaaatctt ttagtttttg ttcttctaat ttaaacattt tcattctcct
     2341 ttcatacttg taacgtttac atttggtaat gaggttataa ccactttaaa ctataa
//
LOCUS       DAEPOZ010000348         2392 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.350, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000348 DAEPOZ010000000
VERSION     DAEPOZ010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.350"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            429..1079
                     /locus_tag="KR440_003565"
     CDS             429..1079
                     /locus_tag="KR440_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBH0700565.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTFKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            1253..2185
                     /locus_tag="KR440_003566"
     CDS             1253..2185
                     /locus_tag="KR440_003566"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBH0700566.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEDSIFIG
                     KNQNPYQEVHHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYRKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
BASE COUNT         1008 a          242 c          363 g          779 t
ORIGIN      
        1 tacattttac ctcatttatg actacacata agtatgaaat taaagatata gaaaagataa
       61 gaataagaga attttataat aaaaaaattt atctaaaaat taatgatgga gaccttttta
      121 aaggtaggta ttggataaga acaaatatgt ttaataatag tatagcatta tatagttatt
      181 tcattgagtt ggaagagtgc ttatatccag attcactaag atttagaaat aaaaaatttg
      241 agaataagaa tatataaaaa taaaaaattg aattgaagcc taacttaatc ttttttgata
      301 agatataagt ttgaacttta tactaaattt ttagaatttt caatcaagac aataacataa
      361 tagctaagca attagaacac aactaagatg tataaggcat gtatatgatg aatgggagga
      421 tttaattaat gttattagag aaattaagaa aagaagtatt acaagcatct ttagaccttt
      481 taaactataa tttagtgaca ttaacaggag ggaatgtaag tgggagagat gaacaaactg
      541 gctatatagc aatcacccca agtggaatgg attatagaaa tcttacacca agtgatattg
      601 ttatagtaga tgtagatgga aatattatag atggaaaatg gaaagcatca gtggatttat
      661 cagaccactt gtatatatat aagcatagag aagacataaa tagtataatt catacacatt
      721 ctacatactc aagttgtttt gctattttaa atgaacctat agaatgcgca agtacaactc
      781 ttgcaaatga agtagggggt tcagtaccag ttgcaaaatt ttcaccacca acatttaaaa
      841 agatgggaaa atgtgttatt gaagcaattg gtgacaaaag agcatgtctt ttagcaaatc
      901 atggcgtaat tgcagttggt ccatctgtag gacatgctct tacagccgct gtaatgttag
      961 aagattcagc aaaagtatat tacttagcaa aatcaattgg cacaccagtg ctactgcctg
     1021 atgaagaaat tcaaagagcg agagatgtat tttttaatgt ctatggtcag gataaataaa
     1081 ttagataaaa ttttaaaagt aatatgaaaa tatagatatt caatgatatt aaggagattt
     1141 ataatgatat aataagttta atagttaaga agtaaaatat gaaatgtaat agtatataaa
     1201 ttcatttaag actaaaaata ataagataaa ataaaaagga gtctgtattt ctatgtatta
     1261 cttattaaac aaaagcacaa atccttactt taatttggca ttagaagaat atctattttt
     1321 aaatgataaa tataatgatg atattataat catatggaga aatgaagact ccatttttat
     1381 aggtaaaaat caaaatccat atcaggaagt acatcatgat gttattgaaa agggagaaat
     1441 tcctatttta agaagaatat ctggaggggg cactgtttat catgatttag gtaatatcaa
     1501 tatgtcattc atacaaaaag ataggcaact tcatgagata gattttttgg agcatactaa
     1561 gtttatgcaa aagatgcttt caaccttagg actagatgta tctataactg aaagaaaaga
     1621 tttattttta aatgggaaaa aaatttcagg ctctgcacag tctataaaaa gaaaaaattc
     1681 tctttatcat ggaactcttc tttacgattc agatttaaat aaattaacta aatatttaaa
     1741 ttcaaataaa gcaactgaat caaatgcaac taaatctgtt tcaagtaaag ttacaaatat
     1801 taagttttta ctcgaaaaag atataaatta ctttttagat tattgtgttg aatatttaaa
     1861 atctaatata aagaatataa aagaacttga attggatgaa gaagatatta aaagtgtata
     1921 caatatagga gaagataaat atagaaagat agagtggaca tatggtaaaa cgccgaaatt
     1981 tcaagtagtg ctaccattta ataatgaaag taaaataaat atacatgtta acagatggaa
     2041 agtatcaaaa ttttttattt cttatgatga taatgtaatt aatatggata agttgttaga
     2101 tttgaatttc tttaaagaag aaataaaaga gagtatttta gaaaattatc cacattacat
     2161 agatttaatc aatttaatat tttaatataa aacctctaat ttgataaaat tgtcttatat
     2221 caaactagag gtttttaata ttcttgcata ttaacaacca ttacaaaatt gtaaaattaa
     2281 aaacaacact attgaaaaat caatatatat tgtaataaat atatgtgttt ttcattagca
     2341 aatagacatt aactatttat acaactgaaa atttaatata taattaatca ga
//
LOCUS       DAEPOZ010000349         2385 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.351, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000349 DAEPOZ010000000
VERSION     DAEPOZ010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.351"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(587..997)
                     /locus_tag="KR440_003567"
     CDS             complement(587..997)
                     /locus_tag="KR440_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700567.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKLESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(1213..>2385)
                     /locus_tag="KR440_003568"
     CDS             complement(1213..>2385)
                     /locus_tag="KR440_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBH0700568.1"
                     /translation="GNTSTENYTLKKASDSTGKNNSDEFEIMSNIENIMMLEMLGILS
                     FIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRRFIVCISEFTRTP
                     LTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFMLVALVVHWFNPL
                     VWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPFSMTICSGFGKTK
                     IEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESFGLTKNEQSDKSG
                     FSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHEESSMSKVTNSEN
                     SEETYVNKSSNSKDVLYTDSGEVSIEQSNYNDNRFVISRKGNLLAVNKEQYRTWIE"
BASE COUNT          799 a          373 c          243 g          970 t
ORIGIN      
        1 tcccccctta aaaataataa taaaattaat caataaattt ataatcatat tattactcta
       61 tgtattaagc aattactatg ccaataaatt ttgatttttt taaacaaatc ttaaaaatcc
      121 taaaatgcta attttcttta acttttaatg aatctaatag ataatattta attttcgtta
      181 tatttttcag aatgtttcaa ttattatttt taagaaaaaa aaataatgtt aacatttctt
      241 aacatacatt aaaaaatgtt aacattataa atttggttta atacttttac tttgactcga
      301 tttactttta ttctacttct tactttattt ttaacttaga ctttattttt ttattactta
      361 tttgagtctt atttatttta ttctcactcc atatgttcaa ttacatttgt ctaaattctt
      421 tgatactttt attacataat tttaacaagc ttaagtgtta tttgggtagt gcagttgata
      481 attatttaaa taatcatcaa ctgcatgttt atatcattac agtagcttaa tgtatttaat
      541 catacttact taggattttg aaatatctta gagattatat ttaacattag tcaattgctg
      601 cataattgta agcaattgaa atttgagacc catcttcatt ttctatagtg ataggcttca
      661 taatttttaa tccttcagtt tttatctttt ctaattcttg ttccattttc ttaattgttt
      721 cctcaaggtc tttttgactt acatctttga ctgcattagg gtctttagca tagtctttta
      781 attcttgaat atgtttagca tactcttcat aagtataatg ctcaacacca tacttttcca
      841 attcagtagg gtcagttatt cctgatttta ttagagattc aagtttaggt aaagcaatat
      901 tagatgaatc atttccattt gcaaatactg ctccagttcc tcctagtacc attgcagata
      961 ccatcattgc acttataagg gattttttta ttttcataac caatcttcct ttcttagaga
     1021 tttatttggg tagtgtagtt taaaatgact attgcaatag ttatcaacca catctttaga
     1081 ctaccatagt agtcatattt gtgtcaagtc tttttaaaaa acttttcaat aaaaaatact
     1141 atctcaaaac aaaattaatt attttgagat agtattttaa ttctaaatct tttatattac
     1201 taagatttat ggttactcta tccatgttct atattgttct ttattaactg ctaacaaatt
     1261 cccttttctt gatatgacaa agcgattatc attatagtta ctttgctcaa ttgaaacttc
     1321 tcctgaatca gtataaagta catccttact attactagat ttatttacat acgtttcttc
     1381 actattctca gaattagtta ctttgctcat agatgattct tcatgctctc taaattcaat
     1441 tacagatact tttgccgtac taaaatgagt ctctccatca cttttagctt tactaacaag
     1501 aatatcagat tctactttgg gagaatataa atcagttgat ttactactta ttgaaaatcc
     1561 acttttatca ctttgttcat tttttgttaa accaaaagat tcaacctcat taatctcttt
     1621 attttctgtt gcaaaaacaa gacctgtccc acaaactaat acagttgaaa ctaaaaaagt
     1681 aacataagtt gattttttaa atttcataat attaactatt ctcttttcta tttttgtctt
     1741 tccaaatcca ctacatattg tcattgaaaa tggattattc tcctctgcta gacttattag
     1801 tgtggttgca taatcagccc tatattcttc accaagtata ctcataacct tttcatcaca
     1861 actaaattct atgtctttat ttagtaaaaa aaacatcacc caaactaagg gattaaacca
     1921 atggacaaca agagctacaa gcataaataa ctttaacaga ttatcaaagc tattaatatg
     1981 aaccatttca tgagttaaaa caaagtctaa agtttgatta ttagcataat tcatttgctt
     2041 tggcaaaata attcgtggtt ttaaaatacc agatgtaagt ggtgtcctag taaattcaga
     2101 tatgcaaaca ataaatcttc taaaacggct attcttacac atccattttt taatgaactc
     2161 atgatttttt atatgaattg aagttttaaa aagattaatt attttaaaat atctaaagca
     2221 taaaagtatg ctaaacataa tagtaactat aaaagacaaa atgcccaaca tttccaacat
     2281 cataatattt tcaatattag acattatttc aaattcgtca gaattgtttt tacctgtaga
     2341 gtcactagct ttcttaagag tataattttc tgttgatgta ttacc
//
LOCUS       DAEPOZ010000350         2335 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.352, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000350 DAEPOZ010000000
VERSION     DAEPOZ010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.352"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            97..162
                     /locus_tag="KR440_003569"
                     /pseudo
     CDS             97..162
                     /locus_tag="KR440_003569"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(678..1346)
                     /locus_tag="KR440_003570"
     CDS             complement(678..1346)
                     /locus_tag="KR440_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBH0700569.1"
                     /translation="MPVLMAIGVPVHLAIGTNKFAASAGCISSAYRYAKSGKINNDLL
                     KKLVPFTIIGSVLGVRCVLSISEEILNVLVVVMILIVAIYTFISKNLGQEDNFEAVNK
                     KNLRLGMLMALIMGFYDGFFGPGTGTFLTFGFIKIYGYDFLHASANTKILNLTSNITS
                     LLLFMINGQVDYKIAIVFALVMIMGAYVGAKVAIKKGSKMIKPIFLVMALFMVVKLVY
                     QTLV"
     gene            complement(1291..1431)
                     /locus_tag="KR440_003571"
     CDS             complement(1291..1431)
                     /locus_tag="KR440_003571"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700570.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGTYKHACSYGNWSASTSCHW
                     YK"
     gene            complement(1490..1759)
                     /locus_tag="KR440_003572"
     CDS             complement(1490..1759)
                     /locus_tag="KR440_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0700571.1"
                     /translation="MNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLL
                     DRSMSISDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
BASE COUNT          856 a          375 c          183 g          921 t
ORIGIN      
        1 tccaatttaa aaagtagtta agtcctattt aaatagaaaa gaatgaaata agaaatatac
       61 tgtataaaaa tgcataaaaa cctctactaa ttagatacaa ctataataga atatatttat
      121 tattataata acaaaagaat taaaaaaatt aatttagcat aaccttgtta aatatagatt
      181 tgaatatgaa tttaaagtta tgtaaaatta aaaatacagg aatgaaaatt ccattcctgt
      241 ataaaatcta acttttgagt ctcaatacaa tcttattttg agacaatctt ttttgtttta
      301 tattttctta tatttaaaat ataacgcata tcttatgcaa catattcata tttttcttta
      361 tattctttca tcgcatcatt ccaagcgtct tgtactgtta cacctctttt attagcaatt
      421 ttttgtgcca gttcaattat cttcatagct cttcttgata tgttcataaa cattcctcct
      481 acaaattacc atatatttca tatatataaa tcaaggttat atttttatga tatataatcc
      541 ttctattgta atattacaat ttatatgtgt attatatatg aactatatta gaagataatt
      601 agaattttta taaagctata tgattagatt aaatataaaa gctttacaat tacttataaa
      661 tttaaaaagt ctataaacta aactaatgtt tgatatacca attttacgac cataaataat
      721 gccattacta aaaatattgg ttttatcatc tttgaacctt tctttatagc aactttagca
      781 cctacataag ctcccattat catcactaat gcaaacacta tagcaatttt ataatctact
      841 tgaccattta tcatgaataa taacaatgaa gttatattac ttgttaagtt taatattttt
      901 gtatttgcag aagcatgtaa aaaatcatat ccatatattt ttataaatcc aaaagtcaaa
      961 aaagttcctg tacctggacc aaaaaaacca tcataaaaac ccattataag agccataagc
     1021 atccctagtc ttaaattttt cttattaaca gcttcaaaat tatcttcttg acctaaattt
     1081 ttagaaataa aggtgtaaat agccactatt aaaatcataa ccacaaccaa aacatttaat
     1141 atctcttcac ttattgataa tacacatctc acacctagaa cactaccaat aatagtaaat
     1201 ggtactaatt tttttaaaag atcgttatta atttttccag actttgcata tctataggca
     1261 ctacttatac atcctgctga tgctgcaaat ttatttgtac caatggcaag atgtactggc
     1321 actccaattg ccataagaac aggcatgctt ataagtccct cctcctcctg ctatagaatc
     1381 tacaaaagca gctaaaaacc cccctataca taaaaaaata actgaaaaca taaaattcct
     1441 ccatcattct ctttcaattt ttcttacaat atatattata taatcttatt taatacttat
     1501 tatccagcat attctttcta tattcatttg gagttatact attaaacttc ttaaatattt
     1561 gtgaaaaata actactgtta ttaaaaccta ctttcttaca aatatcacta atactcatgc
     1621 ttctatctaa cagatatttt ttagattctt ctatcctaag aagatttata tactcagtta
     1681 ttgaaacttt atttctttca ttaaaaatag aactcaaata atttggactt ataaaaaaaa
     1741 catctgatat actattcaaa tttatatttt ctttataata tttttttata tattcgttta
     1801 tattttcaat tagatttcct ttatcttttc tccaaaatgc tctattctta tcaatacact
     1861 ctttggaaaa ttcaaataat acattccata aatcttcatc actgtctaca agtgatattt
     1921 ttttattata tttatttctt aaataagata taagttttaa atcctctaaa tctctaagca
     1981 ctgttctaga caatacaaat atcatttcct gaatagtatc tctaacatca ttatatctta
     2041 atttgtttgt atctattaga ttataaacac cttctaaatt catattttta atttcttttt
     2101 caatataaag ttctaattca aagggatatt cttttgaaga attattgaac atatctatcg
     2161 gattaataat atactcttct tctaataatc tactatttgc ttttttaagt gcctcttcaa
     2221 gtcttatatt ttcttttcta ctctttaata tctcctcatc ttttatacta agttgatttt
     2281 caaaaaaatt cctcttagct atttcctgaa taaattcaga catcattaat aaatt
//
LOCUS       DAEPOZ010000351         2317 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.353, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000351 DAEPOZ010000000
VERSION     DAEPOZ010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.353"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            173..430
                     /locus_tag="KR440_003573"
     CDS             173..430
                     /locus_tag="KR440_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBH0700572.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENNV"
     gene            879..2117
                     /locus_tag="KR440_003574"
     CDS             879..2117
                     /locus_tag="KR440_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBH0700573.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYFSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDTNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISVLSDKGMTNKKNYFNHNHGIQKRNKSATKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYTTYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT         1006 a          192 c          377 g          742 t
ORIGIN      
        1 taacaaagtt ataagttatg agaaatttta ttaaagttaa tacaaaaatt aattttaaag
       61 ctaagaaatt ttttattaga ttcaataaaa ctaataaata tagtttagtc aataatgtat
      121 taaataaaaa ctttgttaag tctataaaaa tatcttaaag gaggtgtaac ttatgaagat
      181 aaaagtggag gatataccag ttcaatttca tgcaatggta gaaataatcg gaattgataa
      241 gttcatagag gttgccaagt tgtatggagg aacaaatact tatataccta cgtataaggg
      301 gctttttaga tatgctagaa atagggatat agtaaaacaa tttaatggag tcaaccataa
      361 tgaattggct attagatata atatgtgtgt aagcaatatt aagcgaatac tcaatgaaaa
      421 taatgtttaa acataatgaa aatagccttg ttaaatgtaa gagtaaagac aaagaagatg
      481 ttttataaag tttttttaga caaattaact aaatttacta cttattaatt taaatacatt
      541 gtatattcta taaaaaaatt tgttaaatac tggagatata ttgagaatac aaagaaaatg
      601 gtcgaaaata tttgttggct aaaaaataga taaaatgata caattgcttg tataaagggg
      661 aaaaataaat aaataaacta tataaataaa gaaatagcaa ttaatatgac ataatatatt
      721 tataaaatta agaatataaa tagatatttt aagggattag ttgttaaatt aggaaatatt
      781 aaaaaaatat atatatttat atttaaacta ttttgttatt tatataaaaa tacttatcta
      841 gttactagca attattagta gaaatggggg aaatagagat gaataaggga ttttacaaat
      901 taaattcagt ggtattgaac tttgatatta agaatgatgt taacaagata agatttaatc
      961 attatataat gagaaaagaa aacatacaaa gacttcaagg aactctgcca aacggacaat
     1021 tttatatgac agtgagaaga gctgctacag atttacaact ttctatttct actataagta
     1081 ggcttgtaaa agagtttttg gatttaggga taataagatt agtgtctaag ggtgtaaaag
     1141 gtaatcgttc tgtatatagt tacgtaagta tagagtttaa tggaagttta tttgatgata
     1201 gcaaatatat ggaacttgac tgtgtatgta atgatgaatt tattgataat agtggcatag
     1261 agtttgattg tgatagcaaa gataattctt ttaatggaga tataaatgta gaaactgtgc
     1321 attgcattaa tgagtatttc tctgatgatg atgtagagca taattataac aatttccata
     1381 ataaagatac tataaaggat aaagccattt ctaatattag cactatcaaa aaatatattg
     1441 atgaatcttt tgatacaaat aaatcagaaa attctaactt acaacaggag tcaataaatt
     1501 tcaactttaa tgatacatgc aatgaaatag ataaaagaac gaataatagt atagaaaata
     1561 atcaaaataa aaggcctatg agattgagtg acatcagtgt tttaagtgat aaaggtatga
     1621 caaataaaaa gaattacttt aatcataatc atggtataca aaaaaggaat aaatctgcaa
     1681 caaagaaaaa agaatcctta aataaattta ataaaaaaaa tatacagcaa agcattataa
     1741 atatgctaaa taaaaaatca ggtaagaatt ttaaaatcga ttctccaata agcataaaac
     1801 ttatcaatga aaggctaaag gagggctatg tcttagaaga tttctataaa gttattgagg
     1861 taaaagtggc taagtggaaa aataccataa tggaaatgta tataagacca gaaaccttgt
     1921 ttagctataa gtttgaatcc tatgtaaatg aaaacatatc tatgaaaagt caaagtaatg
     1981 ttcatccatg taaagaaggc aatagtaatg atagcagtaa ttatatacat tctgattata
     2041 caacatatga tggcagtgtg cgtgagagaa aaaaatatga gaatgtaaga tactggaatc
     2101 ctatatgtga aatttaatta gtttaagaat gtatttatga taaataagat attaatagag
     2161 aatagtgagc tatagcaaat gatatatcaa tagttggtag taagttatat taagcaagat
     2221 attactatca ggtagtgaat tatagtgaac gatatatcaa taattggtag taagttatat
     2281 caaataagat attactatcg agtaatgaat tatagtg
//
LOCUS       DAEPOZ010000352         2297 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.354, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000352 DAEPOZ010000000
VERSION     DAEPOZ010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.354"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            95..1090
                     /locus_tag="KR440_003575"
     CDS             95..1090
                     /locus_tag="KR440_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBH0700574.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRNKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKDYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(1347..1751)
                     /locus_tag="KR440_003576"
     CDS             complement(1347..1751)
                     /locus_tag="KR440_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBH0700575.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     FLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
BASE COUNT          898 a          225 c          380 g          794 t
ORIGIN      
        1 aatatattgc aaagattctc ataatttgtt gtataattaa aggtaacata tttatattat
       61 ataaaaaatt attaaaagtt aggtggggaa agatatgaga aatttaggca atactaacat
      121 gaaaataaag agagttggct ttgggggcat tccaatacaa agaattactc aagatgatac
      181 taacttagtt ataaatgagt tagaaaaaca aggaataaac tttattgaca gtgcaagagg
      241 gtatacaata agtgaggaag caatcggaat tgccattgaa ggaaaaagaa ataaattttt
      301 cttagctact aaaagtatgt ccagagatta tgattcaatg aagagagatg ttgaaataag
      361 tttaaataat tttaaaactg attttataga tttatatcaa ttccataatg ttaaagaaga
      421 ggagtatgat aatcttttta aagataaaat ggcttatagt gcccttttag aggcaaagga
      481 gcaaggtaag ataaaacata ttggtataac tagtcacaat ttaaatacta tagaaaaagc
      541 tatagaagat ggaaaatttg acactataca attcccttac aatatagttg aaggacaagc
      601 agatgaagtg tttaaaaaag ctcatgaaaa aggaattggg ataatagtaa tgaaaccttt
      661 agctggaggt gctttagaca atgcaacact ggctattaaa tatatactat ctaaagatta
      721 catagatgta gttataccag gtatggagag tgttgaacag gttagacaaa atgtcgctgt
      781 acttgaaaat cttgttctag atgaaaaaga taataaggaa attgaagaaa taagaaattc
      841 tttaggtaaa aaattctgta gaagatgtga atattgtatg ccttgtgctg taggaataaa
      901 tataccattg agttttttat gtgaaggtta ttatactaga tatggattaa aagagtgggc
      961 taaagaaaaa tatgaggtta tggatgttaa acctactgag tgtattgact gtgggctttg
     1021 cgagagtaga tgtccatatg aacttcctat aagagaaatg cttaagactg ttgttgaaaa
     1081 gctaggatag attttattta ttgaaatcgg tgtattaaat ttactccgat ttctttttat
     1141 tagatgtggt cttatataca aatattaaat ttacatagtt atagctatta tataaattta
     1201 atatttgttt aatacttatt aaatatttgc ttaatgcttt ttaaaaacat ttgtttgagt
     1261 atagacataa cacaattatt attgttgtat tagtatagtt tcaaatactg gagtatcaat
     1321 ataatgtcga ataacacata aaatctttat ttttcaaaaa tattatagga tatatcgtat
     1381 ttagatactg gataaagttc aatttcaacc ctagggttat ctttatctat aaaatcaaat
     1441 aaaaatactg gcttaagttg tttatcattt tttattattc cacttttttc aataccatca
     1501 caaatacttt ttggataatt atgtaaatcc cccattctct tatttggaaa gaataatttc
     1561 aagactgtta ttagattttc ttcaacagtt tccccttgat actgttggtt tataaaacct
     1621 gcaatcatgt tttcgtatgc aagatactta gaatattttc cctttgaagg catccatgct
     1681 tgaccattta tgttatgaag cttaaaatta gatttactaa taggtctacc tggaattgtt
     1741 attttaatca aatttttcac catccttata cagtatttat tataatctgt atatgattta
     1801 aaagcaaatt gttatttaaa gtgtttttac agggcttgtt tgtaaaattt tccactttag
     1861 atgtagtaat ataagataag tattttgctt acaaatttta aaatttgata tatgatggac
     1921 atattcatat tagactatta taagaccttc ataatattaa taagtatagg tttagtaaag
     1981 agagtaaaaa gaaatccata taagccaagt aaagacttaa taaatggcaa tgtaaatggg
     2041 atattaatta taagaaaaaa tcaatctttt tgcattaact ataggaagaa tgtcaaaata
     2101 taactaatga tatttgaagt tttagagttt atattaaaag gtaagaaggt taagtgggta
     2161 aaaaaggttg aatagattaa aaaggctaag tatctaaaag agttaagtag gtaaaaaagt
     2221 taaataagta aatgaaaaaa gctaagtatc taaatgagtt agtagataaa aaaattaaat
     2281 agataaaaag attaagt
//
LOCUS       DAEPOZ010000353         2291 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.355, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000353 DAEPOZ010000000
VERSION     DAEPOZ010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.355"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            153..1658
                     /locus_tag="KR440_003577"
     CDS             153..1658
                     /locus_tag="KR440_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBH0700576.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            1772..>2291
                     /locus_tag="KR440_003578"
     CDS             1772..>2291
                     /locus_tag="KR440_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBH0700577.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTF"
BASE COUNT          933 a          226 c          358 g          774 t
ORIGIN      
        1 tagaagtaat aaaaaaaaat aattaatata aaaatgtgta caaactaatg aatattgata
       61 tagtaagcta tcatatgaaa gagtatgtac acattttttt tactaatttt tcacaatatg
      121 aacataaata atttacagga aaggaatctt ttatgaaaat tcaaaatact gagtggggat
      181 atattgaatg gaaacatact tatgatgaaa ataatccaaa acaagctatg aatatataca
      241 ttgcagtaac tatgcctggt aagaaacatt ttaatcatgt tcattatggt caagagcaga
      301 tgatatatat tttagaggga gagggtttgt atattataaa tggagtttgg aagccatttt
      361 atcaaggtat gattttttat atagaatctg gtagcacaca tgaaactata aatacaggcg
      421 atagagaaat aaaagaactt attgtttcaa ataatgtaga tgatgtagga gagagtgaag
      481 ttatagatat taatcctaat aattatttaa agaagacctt gataaattat agtgaaagta
      541 cattaaattt gtatgcagca gtagaatcaa ttcgtggtca atttatagac ccgtttaaaa
      601 ttccattaat tatatatgat gattcatgga atatagtttt aaaaaaccct tattttccac
      661 ttttttgttt tgaaaaatgt aatcctatga aatttcctca aaattgtgat tgtatgaatc
      721 aaaaaagtag caatcaattt gtatgtgaat atgggataac tatatataat ataccaatac
      781 tgtacaaaag caatagcata ggtgttataa gaggagggta tgtactatta tctgatttaa
      841 atttagatac agagcataat aatttatatg atattccaga gggagctgct agaagtataa
      901 aacgcctctt gaaacaaata tctaaaaata taataaattt ttgctctttt aatgatataa
      961 gaaaggattt acaagaaaaa gagaagacta tagcaagaac atatcattat ggagagcaac
     1021 ttgaaatgaa tttaaaagta gctcaagata tggttactaa tttaagaata aatcatcact
     1081 ttttatttaa tacattaaat tcaatggcaa gcatagcact agatgatggt agttatgatt
     1141 tgtatagtgc aataatagat ttatcaagaa tgtttagata tacaatgagg tctgatttaa
     1201 gatttgtaga attagaatct gaaatattat acattaaaaa ttatttgaat ttacagaaat
     1261 tacgttatgg agatgcactt aaagtaaaat atcttattcc agagaagtta tataaccttt
     1321 ctgtgccttt taactttatt caacctatcg tagagaatgc atttacacat ggatttagag
     1381 atattgacac agaaaagcga attgaaataa ttgcaagatt agattctcag tatgctatta
     1441 tagagattca taataatgga actatcttag atggaaataa tatagacaaa ataaaggcag
     1501 gtattcgtag taataatggt catggtttgt ctttaatata tacaaaattc acatctgctt
     1561 atggaaataa ttttgatatg gatataaagt cgagtgataa tgaaggaact tacataatga
     1621 ttaaaattcc tattgaaaat tataaggagt atgtatagaa cttgtgaaaa attaataagt
     1681 tttataaatg ttaagaaata tattagatgt ttttatgttg ttttatatat cttacttttt
     1741 tatattatta agctaaaagg gaggtattct catgagaaga gcactaataa tagatgatga
     1801 agaagctgtt ctaaaaataa taacacattt tatagaaatg aaagatatgc caataaaaat
     1861 tgttggtaaa gcaacatctg gtgatgaagc agtagataaa atcattggtt tagagccaga
     1921 catagttttt atagatattc aaatgccaat atacaatggg ttagaagtaa tagaaaaaac
     1981 atctcattta tctaaaaaaa taaactttgt agtaataact gcatttaact attttgaata
     2041 tgctcaaaaa gctttgagat taaatgtaaa agatatactc cttaaaccat tggatatgaa
     2101 agaatttact aagtctgtag aaaaaattat tggatatcaa tataccaata atgatttatt
     2161 aaatgagata ctggaatata taaatctaaa ctatagcaat gacttaaagt taaatgattg
     2221 tgctagatta tttttaacca atccatctaa tatatctagg atttttaaaa gtaatttaaa
     2281 tacaactttt a
//
LOCUS       DAEPOZ010000354         2288 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.356, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000354 DAEPOZ010000000
VERSION     DAEPOZ010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.356"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(168..1001)
                     /locus_tag="KR440_003579"
     CDS             complement(168..1001)
                     /locus_tag="KR440_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0700578.1"
                     /translation="MTIKEVEEKTGLTRSNIRFYENENLIKPLRNERNGYREYSDENV
                     EDIKKIAYLRTLGITIDDIRNIILQKVSLVEVVEKQEKVLETKISDLENAKTMCNKML
                     ESDELSYLNLKVEQYITELPEYWNKNKPVFKLDSVSFLYIWGGKITWGIITIACLLIA
                     ITVFPKLPPQIPIQWNNGVANYVDKKFIYAYPVICVVFRILLRPFIWRWLQIHAFYSD
                     TIADYLTNFMCFVALSIEIFSILFIYNIARHVTIIIFIDILVFIVLLIIGVYKLFKKY
                     N"
     gene            complement(1330..1796)
                     /locus_tag="KR440_003580"
                     /pseudo
     CDS             complement(1330..1796)
                     /locus_tag="KR440_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816343.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
     gene            complement(1861..>2288)
                     /locus_tag="KR440_003581"
     CDS             complement(1861..>2288)
                     /locus_tag="KR440_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH0700579.1"
                     /translation="KARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKN
                     MIRNRKLSRLISDVSWSEFIRQLEYKANWYGRQIVKVGKFFASSQICNKCGYKNEEVK
                     DLNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
BASE COUNT          754 a          387 c          229 g          918 t
ORIGIN      
        1 tttactttaa aatacctaaa ccttttatca gtgaaataaa ccataatata tcgtagctta
       61 ttagaaaaac aaaataattt catatacaaa taataaaata gccactacaa tccataattt
      121 gcagtggcta ctttttacac tatcacttat tacttaattt ttaaaaatta attgtatttt
      181 ttaaataatt tatacacacc tattatcagt aaaacaataa atacaagtat atctataaaa
      241 ataattattg taacatgtct ggcaatattg taaataaaaa gtatagagaa tatttctata
      301 gacaacgcta caaaacacat aaaattcgtt aaataatctg caatagtatc actataaaaa
      361 gcatgtattt gtaaccatct ccatataaat ggacgtaaaa ggattctgaa tacaacacaa
      421 atgacagggt aggcataaat aaatttttta tctacataat tagctacacc attattccat
      481 tggataggaa tttgaggtgg caactttgga aatactgtta ttgcaatcaa caaacaagca
      541 atagtaataa ttccccaggt aattttccct ccccatatat ataagaaact tacagagtca
      601 agtttaaaaa caggtttgtt tttattccag tattctggca actcagttat gtattgctct
      661 actttcaaat tcaaataact tagctcatca gattctaaca ttttattaca catagttttt
      721 gcattttcta agtctgatat tttagtttcg agaactttct cctgtttctc tacaacttca
      781 actaaagata ctttctgtaa tataatatta cggatatcat caatagtaat tcctagtgta
      841 cgtaaatatg caattttttt gatgtcctct acattttcat ctgaatattc tctatagcca
      901 ttacgctcat tacgaagggg tttaattaaa ttctcgttct cataaaaacg aatattagag
      961 cgtgtcaatc ctgttttttc ttcaacttct ttaattgtca ttttaaacac tacctctcta
     1021 ttctattaat ttacaactat aatatagtct taaatatttt cactctataa aattgtatat
     1081 ttagtatatc ttaaaatcaa gttaattatc ttttataaat tttcatacaa taatatctcc
     1141 tttcaataat atctcttttt ctatatttaa aactatctta ttttctataa aataatgtat
     1201 aaacaaggtt tacagtcaag tatttttatt atttttttga tttcataaaa aaaagcatag
     1261 caaatcattt gctatacttt tttactaaat tatttattct atatcttata tgaacttatt
     1321 gtttaactac tacacaatac tagctttaat catcatattt tttgcaaaca tactggcttc
     1381 atatatttct tgttcatctg gatgactttg agattcctca tggttattta attgttggcg
     1441 aatatgttcg ttgtcaggag aaagttttaa catctcttta tattttgctt tcaatccttc
     1501 tgctatctta ccttgacata tgaatgaacc taatatttca ttatcttttg ggataattga
     1561 ttctatcttt tctattatac tagctccata acttcctttt ttacttgctc ccattgttcc
     1621 aaatagtgct atctttttat tgtgaatatt ttcaagcact tgttttgaat ttttatcaca
     1681 aattcccttg tccacccaat agcctaagaa tactatatca taattttcat agtcaatacc
     1741 atactgaacc tcccatgact aaagtcacag ggttcctgct tcatagaatt ttgcatacta
     1801 aaatatgtct tactaattct ccacaggcta tccccgtagt tcctacggtt cttacataag
     1861 ttatttattt tgaattgtta ttagtcttaa tccttctttt aatatattta tacttgcatt
     1921 tatatctcta tcgtgagttt cattacaact aggacaaatc cattctctta tatttaaatc
     1981 cttaacttcc tcgtttttat acccacattt attgcatatt tgtgaacttg caaagaattt
     2041 acctaccttt acaatttgtc taccatacca attagccttg tattctaatt gacgaataaa
     2101 ttctgaccat gatacatctg aaattaagcg agatagttta cgatttctaa tcatattctt
     2161 tacttgtaaa tcttctatgc aaataatatc gttctctttt attaatttag tagataattt
     2221 ttgcagaaaa tcatttcttt gattagctat atgttcttga agtcttgcaa cttttaatct
     2281 tgctttat
//
LOCUS       DAEPOZ010000355         2269 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.357, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000355 DAEPOZ010000000
VERSION     DAEPOZ010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.357"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(126..1325)
                     /locus_tag="KR440_003582"
     CDS             complement(126..1325)
                     /locus_tag="KR440_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HBH0700580.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            1444..>2269
                     /locus_tag="KR440_003583"
     CDS             1444..>2269
                     /locus_tag="KR440_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBH0700581.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHL"
BASE COUNT          796 a          356 c          295 g          822 t
ORIGIN      
        1 tctgtgaaca ttaaataaac ctcttaatag atataactct tgaaaagtca tatctattaa
       61 gaggttttaa taaattttca tgttaatata tataatccaa aaaaattaaa tcttattaaa
      121 acatactatt ttgtatagtt atcgaaatat ccttgtatgt aaataatagg agtaccctta
      181 tctccacttc cagatgttaa atctgctaaa gaacctataa ggtctgttaa tcttcttgga
      241 gtagtacctt gagaaaccat gtttcctgtt aaatcatcag atttattatc tttttcaact
      301 atgtattttg aaattgcttc ttttaactca tcaccagata agtcagcaaa atcattatct
      361 gctaagtatt ttaattttac ttcgtttgga gttccctcta gcccttttgt ataagcagga
      421 gatactactg ggtcagctaa ttcccatatt ttacctacag ggtctttaaa agctccatct
      481 ccataaacca ttacttctac atctttccct gttatttctt ttatattacc ttggattttg
      541 tttacaactt catcgcaatt tatagggaat aattttactg tttcttctgt agctttgttt
      601 gaacctaaaa gtccatactg gtcattgtaa ccactaccat ctacacttgc tgtcattata
      661 tcatcaagtg aatatacttt tttagctcca ttttgtctta aaattctctt tgttctttgt
      721 cttgtatgta tatcacaagt taaaatactt gttgtatagt taagaatagt ttttggattg
      781 tttgcaaata ctatttctac ctctgcacca caatccttga ttaaattctt ataataatca
      841 acataatcta caccagtaaa tgtatgcttg ttatatccaa atagttctct gtatttttct
      901 tctgttaaaa catctgacca aggatttatt cccttatcat caagttcatc aatacttatt
      961 aagtgatttc caacttcatc agaagggtag cttaacatta atacgacttt tctacatcct
     1021 tttgcaattc cttttaaaca tatagcaaaa cgatttctac ttaaaattgg gaatataacc
     1081 ccaacagtat catctccaaa tttatctttt acatcttttg caatattgtc aacgtgagca
     1141 taatttccct gtgctctagc tacaacagct tctgtaacgg caattacatc tttgtcccta
     1201 acttcaaagt tttcactttt tgacgcattt aaaacagaat caacaactat tttaactaag
     1261 tcatctcctt gtcttataat aggtgctcta acccctcttg aaacagttcc aactaatcta
     1321 tccattatct aaaatcctcc taaagaatta atattgtcat attagtacca tgtatattat
     1381 aattcaaata taattataag taaaataaat aatactaata tttgacataa ggagtaatta
     1441 tatatgaata taaaacttga actttataag gtttttaatg cagttgtaaa taataagagt
     1501 ttttctcttg ctgctaagga attgtttatg tcacagccag ctgtaagcca gtcaataaag
     1561 caattagaag aacaattaga tactttatta ttttatagaa acaataaggg agtaaaatta
     1621 actccagagg ggaaaattct aagtgaacat gtcactactg cattaaagtt gatatcatca
     1681 ggagaagata gaataaataa atttaaaaaa ttagaatacg gttctctgaa aataggagta
     1741 ggagatacag ctgcacgttt tttcttgtta aaatatttag aaatttttca taagaagtat
     1801 ccacatatac atgtatctac aataaataga acgagtagag aacttatatc tttactcaaa
     1861 gatgggaata tagatatagc aattataaac atgccaatag aagatgatac actaaatatt
     1921 gttgagtgta ttgaaataca tgatatattt gtatgtgcaa atgattacat agaatacaaa
     1981 ggtaggaaaa tatcattgga agaattaaac acattgcctt taataatgct agaaaataaa
     2041 gcaaattcaa gactttatgt aaatgaatat tttttatcaa aaggtataaa actaaacccg
     2101 gatatagaat tgggttctca tgaattactt ttagaatttg cgtatataaa tcttggagtg
     2161 tcttgtgtta tagaagaatt tagcatagat tatttggaaa atgagaaatt gtttaaatta
     2221 gatataaaag agccaatacc taagagaaat ataggttact gccatttaa
//
LOCUS       DAEPOZ010000356         2250 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.358, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000356 DAEPOZ010000000
VERSION     DAEPOZ010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.358"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            272..1111
                     /locus_tag="KR440_003584"
     CDS             272..1111
                     /locus_tag="KR440_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBH0700582.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
     gene            1108..>2250
                     /locus_tag="KR440_003585"
     CDS             1108..>2250
                     /locus_tag="KR440_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700583.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNIDNPNVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFK"
BASE COUNT          938 a          216 c          394 g          702 t
ORIGIN      
        1 tattttagaa cataaaactg cattaaggcg cgagtactat tttgtattcg cgccttgatg
       61 gtttgagtta tttgagtaaa aactaacttt aataagaaat atgtatttat taagttgata
      121 tttttaatgt gtttagagat tatttaacaa tctccaaata taaaactaga attatttaca
      181 taaaagttaa atagtttgta tagagttaga tttatatagt ataatactta gtgtaaataa
      241 ttaagaagtt atcaaacagg aggataaaac tgtgttagat ataaattctt ttttaaatgg
      301 attttttaat ataatattaa gaatgagtat atatgattta attgacatat ctatagtagc
      361 gtatattttt tacaaaatat ttatgtttat aaaagataca agagcagaac aagtgtttaa
      421 aggtataata tttcttttat tagcaactca acttagtaac acctttaaat tgcataccgt
      481 ttattggata tctcttaaag ctttagatta tggagttata gctgctctta taatttttca
      541 accagaattt agagcaggac ttgaacatat aggaagagca aagtttaatt tatttggaaa
      601 gaatgtaaat acttctgaag aaactctcaa tagaaatata gaagaaattg tagaagcttt
      661 atattcactt tctagacaaa aaataggagc tcttatcata atggagagag aaactagaat
      721 aagtgatata ataaatacag gaactattat tgatgctgaa atttctaggc aactattaat
      781 aaatatattt ataccaaata caccattaca cgatggagct gttgtaatta gagattctaa
      841 ggtaaaagca gctgcatgtt ttttgccatt gacggaaagt aaggatttaa gtaaagattt
      901 gggtactaga catagagcag gaataggggt aagtgaggtg tctgactgta taactctgat
      961 tgtatcagag gaaacaggtg gcgtatcaat tgctaaagca ggtaagttat atagagatat
     1021 ttctagagaa agaatgatga atatattacg tagtaattta aaaacaaata cagaaacaag
     1081 aagttttttc aaaggtggta tatttaaatg ataaatagac taaaaaataa taccaaaata
     1141 aaaataatat catttttaag tgcaattgca ttatggatgt atgttatggc tgtagttgac
     1201 cctgaagaaa ctaaagggtt tgaagatata cctgtatcta taagtaatat gagtgattta
     1261 aaggataaaa atcttgttat atatccagac gaaaaattaa cagcagatgt atatataaaa
     1321 ggtagtttat caaaggtaca aagtgttaag aaagaagata tacatgtata tggaaccatt
     1381 gaagacccaa tacaaggaca gaaggaagta tatttaaaag caaatattcc tcaaggtgtt
     1441 actctagaat ttaagcatga tgttttagta gtaaatcttg aaaaaaacat aaaagaaaaa
     1501 agagatataa aggtagaagt aagtggaaat tcaaaaagaa atataaataa aattgaactt
     1561 agtaaggaat ttgtcgatgt atcaggtcca agaactctag ttaaagaagt caaaagtgta
     1621 agagctaatc tagatgttgg taatgagaca gatgattttt ctaaaaaatt gcaattaata
     1681 ccattaaata gtgatggaac taaggttgaa ggagtaacat taagttcatc ctttgtgact
     1741 gcaaagatag aactattgaa ggaaaaaatt gtcccaataa gagtaagtct aaatgataat
     1801 aaagataatg atgtactgaa aaactataaa ttaagtaagg aacaagttac aataaaagga
     1861 aagaaagaag atatagacaa aataagttat attaagacaa aacctattga cttagctgat
     1921 ttggctaatg gtgaagaaaa agaagttagc ttagaaattc cagagggtat aactcttgag
     1981 gatagctcta taacaattaa attaaatgat acaaaacaat tgagtgcaga atttttatat
     2041 aatgcgggag atattgaatt aagaaatatc ccaacaaata tagataatcc aaatgtaatt
     2101 tctgatgtag atataaaagt tgttgtagag tcaacggagg atttgagtaa gttaaataaa
     2161 aaagatattt cattatatat tgatttatca caaggccctg aggaagatag taaatataaa
     2221 ataaaatatg aaacaacata tcaatttaaa
//
LOCUS       DAEPOZ010000357         2248 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.359, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000357 DAEPOZ010000000
VERSION     DAEPOZ010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.359"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            176..364
                     /locus_tag="KR440_003586"
     CDS             176..364
                     /locus_tag="KR440_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBH0700584.1"
                     /translation="MAKVCSVCDKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            complement(524..676)
                     /locus_tag="KR440_003587"
     CDS             complement(524..676)
                     /locus_tag="KR440_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HBH0700585.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(774..1250)
                     /locus_tag="KR440_003588"
     CDS             complement(774..1250)
                     /locus_tag="KR440_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBH0700586.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            1469..2230
                     /locus_tag="KR440_003589"
     CDS             1469..2230
                     /locus_tag="KR440_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0700587.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLSYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
BASE COUNT          840 a          304 c          296 g          808 t
ORIGIN      
        1 tttccaaata tatttacttt atataaaatt atatatataa ttttatatga taataagaaa
       61 ttcttcaata tgtgtaaggc aaatatatat ttctattgca aatttttgtt atttatgata
      121 atataaatat gtttttagtc tttaaatagt tatcatttta aggaggtgtg cgtagatggc
      181 aaaagtatgt agcgtatgtg ataaaggtaa ggtttctgga aaccaagtat cacactcaaa
      241 taaacataat aaaagaacat ggtcagctaa cttaagaagc gtaagagcga ttatagatgg
      301 agctcctaag agagtaaaag tttgtactag atgtttacgt tctggtaaaa ttgaaagagc
      361 ttagtaacca cgtttaaaag cctacttccg taagtaggct ttttaattta atcattttta
      421 ctctttctca taaattttag gattatactt gaaatccatt ttggtaattt tattgttact
      481 actttcatca atactcaccc cacactttat agttaaaact attttaacaa aaaaataata
      541 atgctccaca tccaataagt attaatgcgg taagtgctga ccaaatccag ccaggaagca
      601 acattgacag aattacagcc attcctgttg ctagacaaag tattcctagc aataccttag
      661 acttgttatt tctcatggaa cacctccaca tagaacctgt atctataaat attttcatat
      721 tcaagtttat gcgacagttt tccattttag accaataaac acattatgta attttaattt
      781 ttcatactag aattattact gaactccgat atagattcta caatatccaa aattcctttt
      841 tttctactta acctttcagg tccactatat aaaaccactg gtataacttt aggaagttta
      901 aaatttttat cttctatttc ttcaaggctt tttttcataa taaaactttt ccaaatttgt
      961 tctctatacg atttcaatct tgggacaatt tcagtatcca tttctgtttg aaattctaac
     1021 actatataat gtgatacttt gccactttta gaaaatacct tataaattat atcaacttct
     1081 tttcctttat attcacatat aaaagatgtt tctaactcta tatttttaag agttaattct
     1141 tttgctattg agacacttgt aaaattttga atcaaactta aaaaactttc tttatctgaa
     1201 tacattcttc tgtactcttc tcgtttttca ttgcttcttt ttaaattcat acagttcctc
     1261 ctaggctata gaccttctat attttagtaa agatagtcta taaattttat tttttaattt
     1321 tcattagtga gtattgacag ttgtgtgtaa aatatccagt aaagatattt ttttatattt
     1381 ttattaaaag atataaattt ttatatttta atatattgac atttacgttg cgtaaaagtg
     1441 tatatttaat ttataggagg tattacttat ggaatataca gttcaaaaat taagtaaact
     1501 agctggtata agtactagaa cacttagata ttatgatgaa ataggtcttc ttaagccact
     1561 taaaataaat tcttcaggtt atcgtatata tggtcaaaat gaagttaata aacttcaaca
     1621 aatacttttt tatagagaat taggaattaa cttagaaaat ataaaaaaca ttataaattc
     1681 accaaacttc gatagtttaa gtgcgttaaa agaacaccat aataaattac tagccaaaag
     1741 aaagcaaatt gatttactaa tagaaaatgt aactaaaact atagctctga aggagggaaa
     1801 atacactatg acagatatag aaaaatttga aggttttaaa gaaaagatga ttgatgaaaa
     1861 tgaaaaaaat tatggaactg aaattagaga gaaatatggt aaagatatta tagatacatc
     1921 aaataaaaag cttaaaaata tgtctaagca agattatgaa aattggcaaa acctaaatat
     1981 cgaaataatt agtaaactta aaaaagcttt taaaactggt gatgcctcta gtgaactggc
     2041 tcaggaagtt gcaaaattac atcataaatg gttgagttac acttggaaca catactctaa
     2101 agaagctcat tcctctctag cacagatgta tgtatatgat gaacgtttta catcatatta
     2161 tgatagagaa caacctggat tagctaagtt tttaagggat gctatagtaa tatatacaag
     2221 agataaataa tataaaataa taaaggtg
//
LOCUS       DAEPOZ010000358         2247 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.360, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000358 DAEPOZ010000000
VERSION     DAEPOZ010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.360"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(553..1449)
                     /locus_tag="KR440_003590"
     CDS             complement(553..1449)
                     /locus_tag="KR440_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBH0700588.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDFLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASRVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKRNYNI
                     SPTKYRNEHREVSNVILKEDMK"
     gene            1591..>2247
                     /locus_tag="KR440_003591"
     CDS             1591..>2247
                     /locus_tag="KR440_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0700589.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     L"
BASE COUNT          777 a          315 c          240 g          915 t
ORIGIN      
        1 tccaaaattt ccctagataa ttatcttaat tatataataa tacttttatt ctacagttgc
       61 aataaaaaac tcgtaataat aacaaaaaaa tctattattg tagaatcttg tatgtttttt
      121 ctatttttat atatttttca ggatgatttt tattataaaa ttttttataa atatataatt
      181 tttttatatt tatttgactt tcttttaatt ctttaggatg gatttaaatt ttctataaaa
      241 atcatactat tttagctgtt tacttgctta tttgatagtt ttatttcttt gattaaatat
      301 ctttcagaat ctaagccaga tgtctcaaaa aaactgattt ataatataat cattttttct
      361 gctattctta tttttccaat tttagtttca tattaaaata tacttttcat agacactaca
      421 ttctttcata ctcattttca catttaacta tacaagtagt cttactcatt tatgtactat
      481 actttattaa tatttattaa gaaaaactca aagatttaaa aatatatatt ttctataaag
      541 tatatttatg aattatttca tatcttcttt taaaataaca ttactaactt ctctatgttc
      601 atttctatac ttagttggag atatattata attcctttta aataagcgac taaaataatt
      661 tatgctatca aagccacatc tcatagcaat ttctgtcata tttaaatctg tttcctttaa
      721 ataatatata gctttatcaa gcctgattcc atttataaaa tcgattgtag tcttacctgt
      781 tatttgctta aatgttctac aaaagtgatg tgcactcaag ttcacacgac tagcaagttc
      841 ttttaatgta attttatcca tatatttttc ttctatcaag tcaaagatac attcaaattt
      901 ttctaaagaa tactttcttt ttataaattc atcttcagaa tatattttat caatatgact
      961 tcttaataag agtactatta attgatagat attagctttt atagataact catatccaac
     1021 ttctttattg tgatattcat caagtatagt tgtaatacat tttaaaatca gaaaatcatt
     1081 ttgaattaaa ttattaaata caatatgatt taaagccaat ggtgctaagt acttagtttg
     1141 taacaaatca acttgattac taaaaaggaa tgtgggctca atacgaatac tatagaattt
     1201 taaattatct gataagcttt ctaaataatg taattcatta ctgtttgcta ttacaatatt
     1261 tccaggcact accttaaaat tatccaatcc acattcaaga tatgctatac catcaataaa
     1321 ataatatatt tgtagatgct catgccaatg tcttttaaag tttgctcctt ttttaatgtc
     1381 atcctttaaa aaaatatcta taaagttttc ttgacctttc ctattaaaat atgtattttt
     1441 atccttcatt attcccccct catcaaaata gtgctaatta taagcaatat agttattgta
     1501 ttcattattt catatcgcta ttatataaaa tgtaactatc attgtaaaca ttaacataat
     1561 gccagtcatt ataataagga ggtaataaaa atgacaaact cttttaaaaa agatgataat
     1621 agaaaaaaaa tcacagtttt atttgtagtc attgccatga cttttatggc aacattagat
     1681 tcgagtatta taaatgttgc tctcccagtt cttgcaagca aactaaatgt atctctagca
     1741 tctattgaat gggtaatagc aagttactca attattattt gttcaacact attatttttt
     1801 ggaagacttg gagatattat tggtaaatca agagtattcc aagtaggtac tattctcttt
     1861 acatcagctt cattactatg tggattaagt aattctttaa ctttacttat agtttgccgc
     1921 tttattcaag gaataggcgc ttctgcctac atggcaaaca atcatggaat tatcacagaa
     1981 ctatttccaa aagaaagcag aggaaaagcc ctagggattc tagttactgc agttgctatt
     2041 ggaaatatgg ttggaccatc tgtgggagga tttattttgt caatatttga ttggaatgtt
     2101 atatttttta taaatattcc aatcggtctt attgtaatct ttttaaatac aaaatttctt
     2161 cctaatagta aaaaatcttc agaaaatatg gataaaactg gtgctatact tcaattttta
     2221 ggaacaacat tattttttag tgctcta
//
LOCUS       DAEPOZ010000359         2237 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.361, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000359 DAEPOZ010000000
VERSION     DAEPOZ010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.361"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            206..2224
                     /locus_tag="KR440_003592"
     CDS             206..2224
                     /locus_tag="KR440_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBH0700590.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLIMERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIVNTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
BASE COUNT          940 a          179 c          356 g          762 t
ORIGIN      
        1 tatatttttg aaaaaatatt tgaaacaaaa aaaatatggg agcatacgaa aatatatcgg
       61 ttatatataa aaaaaatacg tttatatatg aattatatag ttctaaatat aaaaaaagtt
      121 aaagtcctac cgtaaaaaag cgcattttga ataagataac aaatatgata aaattggtct
      181 tgttgaaatt tttagggggg atatgatgac aaagaaacta gtaatatcaa aaacaaagtt
      241 gtttattttt ataatagcat ttatttctat atttatatat ttatttggaa gtaaaaatac
      301 acttataggt gtaggtatag taacagctat gctaacactc ttggaaagag atttaactat
      361 ttctccaatt aaaaatttac tgaagtattt agcaataaat ataatcttag gaatactatc
      421 attttttgca gtacaaaata tgtatttagg agttttatta aattttatag cattatttat
      481 aatagggtat gtatttagtt atgacttaaa gagagcagta tatgtacctt ttggtctaat
      541 gtatatattt atggtaagta tacctgtagg aaaaagtgaa tttcctatga gattgtcggc
      601 tttagctgtt ggagcagtta taataatgat tgctcaattt gttatgaata gaaataggat
      661 gaaaaatgtc ggagataaag aactgatatc tatatgtgat gaattattag aaaaaattag
      721 tcttttaaaa aatactatta atgatgatag ttctataatt aaatctagta tgagaaaaat
      781 agatagctgt aattatagaa taaattctat atctaaaaat ttgaaaatgg ttatttttga
      841 taatagaaaa gatgactttt atatcagtat tagaggtata gacataatga atattttatt
      901 ttcattagaa agaataagtt taatcatgga aagatacaaa aaggatagta aagaatttga
      961 agatgaagat ataaaaaata ttttagaaga aagtagcata aatagtaaat cagatgttct
     1021 agttgtagca acgaaagaaa taaactatat taagatgtgt ttagaaaata aagataccat
     1081 aactgataaa gagatattag gatttagaga ttatgttatt gctcaggata ctaaaaatat
     1141 aaatttaaaa gaaatttata gtgttttaga aaatttatat gagtttttag tagattataa
     1201 aaaagtaaaa tcaagagacg aaaagaaagc tgaaagaaaa tcaaagatac ctcatgaatt
     1261 taaacgacta tcaatatata agaaaaactt taatttaaat tctataagat tttcttatgc
     1321 agtaaagatt gctttagcta cagctgtagc gggttttatt atggattatt tccatttaag
     1381 agatggaaga tggataatgt taactgtctt ttcattaaca caaccttacg cagaaaattg
     1441 tatacaaagg tctagaaaaa gaatagaagg tacatttata ggagctgtaa tatttattgt
     1501 attattttca ataataaaag atagtacatt aaggtcgtta atagttcttt tagcagggta
     1561 tataaactct tatgtagttg attatagaaa attaatggta tgtgtaactg tttcagctct
     1621 tggttcagca gtagttatgg gagacccaaa tgttttaacc ataagcagga tttcatatgt
     1681 agcattagga gcaattattg cattaatagt aaataagttt atattaccat atgatgctaa
     1741 aacaggctat caacatgtta tagaaatgta taaaggaata gttaaaaata taattgatga
     1801 agtcaataag tctatagaaa atgtagctga tgtttactat ataaaaaatt tattattgat
     1861 accatcactt attgaagata gacttatgtt gataaatgct atatataaag ataaacatca
     1921 agaggatttt ttagaaaatc agaaattact aataagtaat atgtataatc tatatataaa
     1981 tgttaataaa aataaaataa aagatgaaga tgttgaaaaa atattgagag atactaatta
     2041 tatttcaaat tacaatgtag ataagtatga tgaaggaaga agtgtaattt tagagagtat
     2101 tgtaaatact aaaagtctag gtgacaagat aatatgtcta aacttactgc aaactctaaa
     2161 tggtgtcaaa gaaatgtata gaataagtaa tataactaaa gtttcaataa aagaagcagc
     2221 ttagatatta aattata
//
LOCUS       DAEPOZ010000360         2235 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.362, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000360 DAEPOZ010000000
VERSION     DAEPOZ010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.362"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            70..210
                     /locus_tag="KR440_003593"
     CDS             70..210
                     /locus_tag="KR440_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700591.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(207..578)
                     /locus_tag="KR440_003594"
     CDS             complement(207..578)
                     /locus_tag="KR440_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0700592.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
     gene            1127..1384
                     /locus_tag="KR440_003595"
     CDS             1127..1384
                     /locus_tag="KR440_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700593.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(1494..2174)
                     /locus_tag="KR440_003596"
     CDS             complement(1494..2174)
                     /locus_tag="KR440_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBH0700594.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYFIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISKKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
BASE COUNT          889 a          254 c          262 g          830 t
ORIGIN      
        1 ctttattaaa aatttgttgg gaatttctga ctagcattaa gaacgtaagt ttgttggggg
       61 atggtatatg tggaaaagtc aaacactact gttacaaaaa aagaaaaaat tgaagatata
      121 ggaatgaaat tagaacattt attgagtgaa gatattactt tttatgaatt attacttgag
      181 attttgtata aagaaaaaga agagaattaa ttctcttctt taaggaatct ttttaaatag
      241 gcattatcaa tcattttttc tatagcttct ctatcttctt tttcaagaga atatattttt
      301 tctaagagct tttttgtttt ttcacttact gataagttct ctatgattaa tttatcttct
      361 ttgcttattt ttgtattatc atttacgcct aataaataat ctgttgatgt ttctaatgct
      421 tttgctaatc taactattat ttcactttta ggttctctta aattattttc ataacgtgac
      481 aagctagcct cagttatacc gactttagta gcaagttctt tttgatttat atctaattct
      541 tttcgtagat ttgctattct atctcctaaa ctttccatat gtacatcact ccttaccgaa
      601 attataagtt attttgataa aaagtaaaat aaaattacca aaaatataaa aaaaaagggt
      661 taaaataata aaatatattg aaaattacca tttgtatatg tataatatga attatacaaa
      721 tggtaatttg atgagaggtg gtatgttgaa ataagaaact gaattaatta ataaatatat
      781 agaaagtttt gaaaaccctt aattgagata taaacaatat gtacaaaatt ttttgcaagt
      841 aaaattacca aatgtataaa ataaatcagt agcatagata attttatgct ttaatacatt
      901 agattattta atttagttat aatggtcaaa aatgcaatct acaaataaaa caaagtatta
      961 tcaaaaaagt attctttaag agaatttttt attagagatg taatttatat aaataatcgt
     1021 attaaaaaat aaaatactaa gtttaaaaat ttaaataatc tataaatcaa ttataaaata
     1081 ttatttctta gaaattaaaa tgaattaagt aaatggagga attgatatga taataattag
     1141 aagtcaagat aaaactaatt taatgcagat aaaccaaatt aaaatagatg gtagtcaagt
     1201 ttatgcagta tttgaaagta aaattgatac tgtaaaaata ggagattatg aaaataatac
     1261 aagagctata caagtgttgg acaatataca aaacttcatt gaaaatggaa caaaatatga
     1321 ttatataaca tcaaataaag tcagatacaa tgttaataaa atatttcaga tgccagctaa
     1381 atagtgagtg tgcattttag tttaataact aaagcccact ttcttttgta gtatgaaaga
     1441 gggctttaca tatttgtaaa tatcgaagta cacttgtttt atcaataaat tatttaataa
     1501 ggtataatgg accaattaaa caataaaaat tctcctaaaa cagcaagtat taaatatata
     1561 atagcttcat ttttgtaaag agaggtaatt tttttgctta tgtcatcatc aattgtaata
     1621 gcaatttcat cttgaataat atttttatat atagatggga aaatatcttt tttctttata
     1681 agattaaata aattatttac acctacccat ataaaatatc ctaaaacagc acctaatgtg
     1741 ttcatcatta aatcatcaat atctgtagct cttaagttaa acaattgaca aagttcaatt
     1801 gctaatgaaa aacctaatcc agttaatgaa acattgataa tatttctaaa atttttccaa
     1861 attaaaggta gtaaaaatcc taaaggcata aacataatta tatttaaaat atatgttaag
     1921 atgccaactg agtcaaaagg tattagattt atttcatcaa ttctaaaaag cccactatac
     1981 cttcctatat cccatatgct accaattcca gctttactaa gtgctagata gatatataat
     2041 aaaaaaatat atacccatac taaatgtatc attacattct tttcaacttt caaatttttt
     2101 cgttttataa cgactatttg gtatattaag catggtaata taacgcaaat aaatgaataa
     2161 aatgtccata tcattttatt ttgtcccctt tcaaatttat gtttttagtt ttataagtac
     2221 tatctttata ttaaa
//
LOCUS       DAEPOZ010000361         2231 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.363, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000361 DAEPOZ010000000
VERSION     DAEPOZ010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.363"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..373
                     /locus_tag="KR440_003597"
     CDS             <1..373
                     /locus_tag="KR440_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700595.1"
                     /translation="IAISFLLIISIINPLQVEAEAIIESSVYISSIKENYTYDNTKFK
                     TIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRRNARRHNILTKLVLSIIV
                     IFLVIVLVVLIFIKRKKKKFY"
     misc_binding    809..1072
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1223..1774
                     /locus_tag="KR440_003598"
     CDS             1223..1774
                     /locus_tag="KR440_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HBH0700596.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT          931 a          214 c          308 g          778 t
ORIGIN      
        1 gatagctata tcttttctac ttataatcag tattataaat ccattacaag tagaagcaga
       61 agctattatt gagtctagtg tatacatttc ttcaataaaa gagaattata catatgataa
      121 tactaaattt aaaactatta aatctaaaag tagacataaa agtaggaaat ataataaaag
      181 taaagtaaag ttgaaaaaac gaagtataag aactaaaaag tatggatatc aaactataaa
      241 taaatataaa agacgaaatg caagaagaca taatatccta acaaaattag tattgtccat
      301 aatagtcata tttttggtta tagttttagt agtgctaata tttattaaaa gaaagaagaa
      361 aaaattttac taaaaataat taaaagaata tcatttttct aatcattggt atctatttaa
      421 taatatgatg taattaaaat ggagtaatat tctgtttcaa aggaggcata taaatacagg
      481 aatgagtatt aaatgatggt taattaagta aatttattga taaatgtact taattaactt
      541 tgaagaattt aattcctcat gaatcaaaat ataaaattac atcaacagtt attcacaaaa
      601 ttaatataag aagaattaga gccaaagagc agactattag accattaaag taattattca
      661 tagaattttg tatatgaaaa gaaatgtata gtataattta ctttttttat aaaaaataga
      721 ttacaaaata tattttttat aaaaaggtat tgacattttt tagaaaaaag aatatcatta
      781 ttttaacaac aaaattcaat acaataaatt aaatcctatg acaaggaaga gtagttaaat
      841 ttaggttcag tatagagagc tgaggatggt ggaatcttag caagaaaaaa tttgatgaat
      901 ggcccttaga ggaataaatt gaaatcatag ttttgagagt agactttatc gtcagtcgca
      961 cgttatagcg aataaggtat gtaagtacct gataatgaga tgtatgaaaa ttgaatttta
     1021 tattaatttt tatataattt aggtggcaac gcggataatc tccgtcctat tagtttagga
     1081 cggagttttt tattttactg aaaatttaaa agatagaagg tggtgagaaa gatgttatga
     1141 atttcaaaag ctaaagatga tgatatagta ggttggcaaa aaaataaatt gaaaattaaa
     1201 aaaataggag gaagtacaaa aaatgaaaac aaatacaaaa aaattaactt taaatgcaat
     1261 acttttagcg atgggattat taatacatca aatcacacca gctataggat taccaatgaa
     1321 gccagatgta ccactagcaa tgttatttgt aatattggtt ttaaatagag atgattataa
     1381 aacttgtttg atagcgggta tagtaacagg aatatttaca gcacttacat caagttttcc
     1441 gggaggtcaa attcctaatg taatagataa aactcttaca gcaaatatag tatttttatt
     1501 aatgaatatt agttataata tgccttttat aaaaaaatta gctaagaaaa cacaagatac
     1561 tgtagtagtt gcaataataa tgccaatagg aactcttgta agtggaacta tctttttatt
     1621 agcagctcaa gctatagttg gtcttccagg agcttcattt acagctttat tcttagcagt
     1681 agtattacca gctgttttaa taaatctagt tgcaggaata tttttattca agattgttag
     1741 tctatcaata agaagagtta gttatcaagc ttaactacga aataatattt tactttataa
     1801 agcattataa taactaaata acataagaat aaagtagatt ataactattt tattcttatg
     1861 ttgtttaaag atttggtatt atatatttat ttttaataaa atatttatat agtaccaatt
     1921 acacttaatt taacagataa attatcatac ctatataata ttcttatatg tatctcattt
     1981 gtagttatta aatataagat atatttaatt gacttttcaa gataaatttt tatattaatc
     2041 tcctctgatt tctcattttt tgaatacact aaaatttcca attaaatcaa ttatatattt
     2101 atagagtttt aaaaatatag taatgtatat attttttgtg gaaaacttgc ttaaatttag
     2161 caaaaaaaat agataattca tagtataaaa taaagtttag taaaaaattt taatagaact
     2221 ataaaagtct g
//
LOCUS       DAEPOZ010000362         2188 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.365, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000362 DAEPOZ010000000
VERSION     DAEPOZ010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.365"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(213..764)
                     /locus_tag="KR440_003599"
     CDS             complement(213..764)
                     /locus_tag="KR440_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0700597.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            955..1917
                     /locus_tag="KR440_003600"
     CDS             955..1917
                     /locus_tag="KR440_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBH0700598.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
BASE COUNT          821 a          244 c          372 g          751 t
ORIGIN      
        1 attatcacaa taataaaaaa gaaaaaggag tattttgtac tccatttgtc aaaaatataa
       61 aacttttata taagcttatt ataacataat tgataaaagt gtaaagctat ataatgaatt
      121 tataaagaaa aataaaataa aaaagataaa tcctaatcaa aggatttatc tttttgtata
      181 gaaaataatt attctattat taaatggcat ggctaaactg gataataatt tattattact
      241 gctttcaatg ttttttctcc ttgattatgg tatgtatgaa ttgattcagc tgaaaaacaa
      301 atagagtcat tttctctaag ttgataaata gagtcattta cttctaattt taattgacct
      361 tctataatca taatatattc ctgtgatttt tcagaatgac caacagatgt ataactatga
      421 ccttcttcta attccatttg aaataattcg aagtttctag ttggtgtatt gggataataa
      481 caataaagac gataatcttt attttctgaa acttgtacat ctatatcagt ttttgataca
      541 ataaaagttt cattttgagg ttgttccagt attgcagtat atggaacatt tagtccactt
      601 gcaattttcc aaattgtgtt tactgttggg tttgagtctc ccttttcaat ttgcgaaagc
      661 attactttac ttacgccaga taattcagca agttgaccta gacttaagtt acgttcatta
      721 cgtaatctat ttaaattttt agcaataaca gtattaatac tcatagtaac tccttaaaat
      781 gtaaattatt gacaaatata atttacgaat gtataattta tttaacatta tttataattt
      841 acaaatgtat aatataatga acgttaaata cactgtacat ccgtaatata aattatactg
      901 cataaagagt tgtttttcaa cggatgatag ttgagagatt aagggggaat tgtaatgcgt
      961 gtacttaata atgttacaga ggctataggc aatactcata tgttacattt atccaaaata
     1021 gcagatgaat ttggagttca aggtaatatt tatgcaaaga tggaatactt aaatccggga
     1081 tttagtaaaa aggaccgtgt agcattacaa atgattaccg aagcagaaaa tgcaggttta
     1141 ttagttccag gtcaagcagt tgtggaatta acaagtggta atactggaac aggactagca
     1201 atagcttgtg catgtaaagg atataaattt atagcgtgta tgtcaaaagg aaattctatg
     1261 gaaagagcaa gaatgatgaa agctttaggt gctgaagtag ttcttgtaga ccaaatgcct
     1321 aattcagttc acggacaggt atctggtgaa gatttggcac ttgtagaaat tgaggctcaa
     1381 aaaatagcaa aagaaagaaa tgcatttaga gcagaccaat ttaatttaga agcttgtaat
     1441 catgcacatg aaaagtatac agcagaagaa atgtgggaac aattggatgg aaatattgat
     1501 gtatttgtag attttcttgg aagtggtagt acttttgcag gatgttcaaa aacacttaaa
     1561 aaacataatt cttctatcaa atgttatgtt gttgaacctg aaacagcagc aatttattca
     1621 ggtaaagaaa taactgacca agggcataaa atacaaggtg gaggatattc tatggatttg
     1681 ccattagtag acaagaaact tattgatggt tatattcaag ttacagacga ggaggcaact
     1741 gatgtagcaa gaaaattagc taaattagaa ggtgtatttg caggattttc ttcaggggca
     1801 aatgtgtgtg cagcaattaa gttgttaaag agcagtgaaa aaggaaaaaa tatagtttta
     1861 actcttaatg atagtggttt aaaatattta agtactgatt tatatgaaga attgtaataa
     1921 tagcacaata gttatgcaat gttttttaaa ttaagtatat agatttaaat tataaaaagt
     1981 atctcaatgt gatttttgaa agttataaaa gagatacttt tttattttgt aaatatggag
     2041 tatcctactt ataagtatta agctatatag atattgatga ttagctatgc aataaaaaag
     2101 agtatgtgtt tttgcattta tacatcataa cttggaataa tacatattaa tgttctaaat
     2161 gtatttaaaa catatgataa aattagaa
//
LOCUS       DAEPOZ010000363         2179 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.366, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000363 DAEPOZ010000000
VERSION     DAEPOZ010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.366"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(158..1450)
                     /gene="brnQ"
                     /locus_tag="KR440_003601"
     CDS             complement(158..1450)
                     /gene="brnQ"
                     /locus_tag="KR440_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBH0700599.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLI
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIKFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGVK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          725 a          373 c          243 g          838 t
ORIGIN      
        1 ttattgtctt tataaatcat actcctccaa aaataaattt atcaaatgag attaataatg
       61 ttcttttctc cacaataatt taataattct tttaaatata aaaaggaatt gccatataat
      121 ttaaaggcaa ttccacaatt atcaattagt tttattatta tgcagtttta ctgcttttag
      181 atggaataaa gtttcctatt attcctgcta ttacaactgg aactatccag ttaaatccta
      241 atgaagcaaa tggtaagcta ttaactagag gaatttttac accaaaactt gttgcaactg
      301 ttaatacact tactaataat gccatgtatg cagcacattt gaacacgttg tcatttttaa
      361 ttttattact aaataatgct agtataacta gagttatagt tgctggatat accatactta
      421 gtataggaga agagaatttt attattgtac ttactccaaa gtttgatatg attgcactaa
      481 atacacaaac tactataact atatgctcat actttaattt tccatctgta agtgttgaga
      541 aatattgacc agctgctgaa gaaagaccta tagctgttgt taaacatgct aatgcaacta
      601 taatagccaa taaaatttta cctgtagttc caagtaaagc tgctgttatt gcaactatta
      661 aagctgtttg ttgaacatct tgtccatatt gttgagacac agttgctcca agataacata
      721 atccaccata aactaatgcc attcctatta ctgcaattac acctgcactt attgtggctt
      781 tcatcttttc tttctcacct gtataacctt tagctattac tgacatgatt ataattgatg
      841 caaatacact agctgccaat gcatccattg tttgataacc ttgagttata ccttctgcaa
      901 atacattttg aattaaagaa ctgtctctta tttctcctaa tggagatacg atacctttaa
      961 ctattaatac tgctaaagct ataagtaaag ctggagttaa tattgaccca actatatcta
     1021 caacttttga tggtcttatt gttaaaacca atactagagc aaagaatact atagagaata
     1081 ttactggact tattccagaa cctattgtag gcaatatacc catttcataa gttgttgctg
     1141 ctgttctagg tattgctaaa agtggcccta tacagattac cattgcacat cctaatgcaa
     1201 tacttaatcc tttgcctgct cttgagaaca tcttcgatgt gtctccattg aacttagttg
     1261 ctgctataac tgctaataat gccaaaccac catctgcaaa aaggaatcct atgaaagcta
     1321 ttagccaact acttccagat aaaactccta gatatggagg gaagattaag ttccctgcac
     1381 caaaaaacat tgcaaatagt gcaaatccaa ttacaattac atctttaaaa ttgatttgtt
     1441 tctcacccat ttaaagcacc cccatttgaa ttttactacc ttttactaaa aaataagtcc
     1501 aatctcttca ttaaatgaaa accttttgac gagattttat ttaattatac atttttgact
     1561 taaatttgtc aattatgttt cataatttat aaaatcttca caatatttaa tacaactttc
     1621 ttaattaaac aaaatcaacc tgtatacttt aaacatagtt ggttttttgt tttttctcta
     1681 attttccttg ataaaattaa ctattcagaa caattaaaat attaagactt tttagattat
     1741 tcgttttatt attaagttgt attgctttat atactgtata tatcactctc ctttttacat
     1801 tcatataatc tttttaattc aatttaaatt ttaaatattt tgtgtttatt atataatctg
     1861 aaaaaaaagc agtcaatagc atttttaatt tttttaattt tcaaaattat gattcttaaa
     1921 aaatcgtttt ccttttatca taacacctat taacgttaat tcttgtagta ttttgtattt
     1981 attttgctaa attgacagtt aactttattc aatttttatt ctactcacgt agatttacct
     2041 cttgtctttt agtcttaaaa ccattttttc tgaaaattcc ttattatgat ttatcaactt
     2101 atttttcttc atagcaatta ctttctaact tccaacttac ttaaactttg cagttgctag
     2161 tattcacgtt tacatactt
//
LOCUS       DAEPOZ010000364         2175 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.367, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000364 DAEPOZ010000000
VERSION     DAEPOZ010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.367"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            690..1961
                     /gene="serS"
                     /locus_tag="KR440_003602"
     CDS             690..1961
                     /gene="serS"
                     /locus_tag="KR440_003602"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBH0700600.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKHKLNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGLDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
BASE COUNT          933 a          214 c          346 g          682 t
ORIGIN      
        1 ttataagtat aattttattg attctacctt tttaaataac tgtcgattta ttaaagttat
       61 ctatgtacta tattaaacaa ttaagtagtt attaattgca cttattagtt tattaaaaaa
      121 ctatgtatat tactttgcag taagactttg gaactaaatc tataatagca aatttaaatt
      181 tattaaatag attaccttat aacatttcat ataaaatata ttgtgaatct gagaaaatta
      241 ctatttttct ttgagtattt tttataagca gttctaaaag attcaatttc atacctatta
      301 cttgaagcat tcattaaaat aattctaatt taagaaataa ttctatccaa ataatagata
      361 tataggacta tgttataata taaaaaaatt tatataaaag ttaataacta tacaagattg
      421 ataaagaaaa agtcaaaata gataattgat tagcaaaata gatataatta taatgaagtt
      481 aaatctagca atattctcct aaaattaaat ttcatttatt taattaaata taatttgaca
      541 atggacaaaa aagtgagtaa aatataaata aaataaaaat aacaggttcg gataaagaaa
      601 aaggtttctt aacctcaagc ataaagtata ttatctaagt tatttgagat taagagataa
      661 atccaatctt aggtactgag gaggaaaaaa tgcttgatat aaaaagaata agagaaaatt
      721 tagattgtat aaaaaaagca atggaaagaa ggggcgaaaa agactttaac cttgatgaag
      781 tagttaagtt agatgatgaa agaagaaaga ttcttcaaga agttgaagtt atgaaacata
      841 aattaaatac agcatctaaa aatataccaa atcttataaa agaaggtaaa gatgtagaaa
      901 atgaaaagat taaactaaaa gagttatctg ataaaattaa ggtaatagac caaaatctta
      961 aagaagtaga agataagatg gaatacctat taatgagaat accaaatgta ccacatccag
     1021 aagtaccaca aggtgaaact gatgaagata atgttgaggt aagaacttgg ggaaagacta
     1081 ctacctttga ttttgaatct aaggctcatt gggaaattgg aacagaatta ggtatacttg
     1141 attttgaaac agcgagtaaa ataactggtt caagattcac tctttacaaa ggattgggtg
     1201 ctagattaga gagagcatta ttaaattttt acttagacac gaatacaaaa gtaaatggat
     1261 atacagaagt cattccacca tttatggcaa atagaaatag ttttttaggt acaggacagt
     1321 tacctaagtt tgaagaagat atgtttaaaa tagaaggtct ggactatttt atgattccaa
     1381 catcagaagt tcctttaact aatatacatg ctaatgaaat acttaaattt gaacaattac
     1441 caataaatta tacagcttat actccatgtt ttaggtcaga agctggttct gctggaagag
     1501 atacaagagg tttagtaaga cagcatcagt ttaacaaagt agaaatggtt aaaatagttg
     1561 caccagaaga atcttacaat gagttagaaa agcttactaa caatgcggaa actatgctac
     1621 aattattgaa tctgccttat agagtagtaa agatatgtac aggtgattta ggatttacgg
     1681 cttcttttaa atatgatgta gaagtttgga tgccaagtta caataggtat gttgagattt
     1741 catcttgttc aaactgcgaa gatttccaag ctagaagagc aggaataaga tttaaaagag
     1801 ataaagattc taaagctgaa tatgtacaca cattaaatgg ctctgggtta gctattggaa
     1861 gaagtgttgc agctatatta gaaaattatc aacaagaaga tggttctgtg gttgttcctg
     1921 aagttttaag accatatatg ggagtttctg ttattaaata agagttaaat tatgtatttt
     1981 taagcaatac aataatatag aagtaataaa aaataattaa tataaaagtg atacaataat
     2041 atagaagtaa taaaaaaata gttaatataa aagtgataca ataatataga agtaataaaa
     2101 aatagttaat ataaaagtga tacaataata tagaagtaat aaaaaaatag ttaatataga
     2161 agtgatacaa taata
//
LOCUS       DAEPOZ010000365         2127 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.368, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000365 DAEPOZ010000000
VERSION     DAEPOZ010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.368"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(95..493)
                     /locus_tag="KR440_003603"
     CDS             complement(95..493)
                     /locus_tag="KR440_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBH0700601.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            complement(496..2043)
                     /locus_tag="KR440_003604"
     CDS             complement(496..2043)
                     /locus_tag="KR440_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBH0700602.1"
                     /translation="MNKNSMTKIYSLNKIIFIIIGILGIVLLTRHIEVDIKVLILILL
                     TLFSINVFISYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIMILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIINLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDK
                     CMTVEEE"
BASE COUNT          716 a          355 c          188 g          868 t
ORIGIN      
        1 agctattctc atactcttta gacagtagaa aattatcttg agatatatca tccataaccg
       61 aaaacctcca attattatca tagctgaaaa ttttttattc tagtatttgt cttatagatt
      121 ttctcgataa aagtttataa atttcttttt ctatttctag caattcacga tttactttac
      181 aaccttttaa gcaattactg caattagata catatctcgt acatttattt atatatattt
      241 ttccatcaat tacttctaca atagttctta gacttattgt atccaatgac acatttaaca
      301 tgtatgtctt gccatcttct ttgttaaaag taagaatatt gtttgcacaa agctttctaa
      361 gcagtctgta tagtgttagt attggtatat cttcactttt taatatcatt ttagcatcta
      421 ttggtttatc ttctccatta ttaaatatag ctcttatgat tcttattgaa taatctacat
      481 cttctgaaat catccttact cctcctcaac tgtcatgcat ttatctaaaa gagtatcttt
      541 cagatttata taacttccgt cagtaagaag ttttttataa ttcatctcat ccaaaggttt
      601 aaaaaataca taaccttgta tgaaattaca tcccagttca cttaagactt tgacctgatg
      661 catatcctct accccttcac atataacagt tacgtttaaa tcatgtgaaa ggttaataat
      721 tgctttcacc ataaactttg tttttgtatt tgtaactata tccaatataa gtgacctatc
      781 aatttttata atatctagtg gtatcccctt aagattgata aaatttgaat tacctactcc
      841 aaaatcatct aatgatatat tcatacctaa agatttaatc tcataaagcc tttcacttat
      901 aaattttaca tctcttagcg ctgtcgtttc tgtaatttct aattctataa gttcttttgg
      961 aatattatat gtattaaaca tcagtttaaa aaaatcaact aaatcatttt tatatagttc
     1021 tactctagaa agatttattg agactggtaa gacatttata ccttctgaaa tccacttgtt
     1081 aatggtaata aaaacttctt gaattagcca tctaccaata ttgtttatca gaccattttt
     1141 ttcagctata ggtataaact tatttggtcc aaccatgcct aatgatgggt gcaaccatct
     1201 tatcaatgcc tctgaaccta taatgttacc tgtacttgaa tcaactttag gttgatagtg
     1261 aattacaaac tctttatttt taatagccaa gaataaatct ctctctattt tcataattga
     1321 ttgtatttct tcttccatag cattatcaaa ttctacatac tctatgtgct ttagtccttt
     1381 tgatttaagc cttgccatat cagcattatc tatagctttt tttatatcaa tttcattatt
     1441 gttaaattta taaattccaa tagaagcctc aatatttata tccattttaa aactgtcaat
     1501 ctgacttaat atcataattt tattcttgat aagatttact aaataaggaa tcttctgttc
     1561 cctattttca agctttatta ttaatacaaa aacatcactt tttaactttc cataaatagc
     1621 ttcttttcca aaataatttt ttaagttact taaaatatta tttaacactt catcccctag
     1681 ctcataacca tatatatcat ttataagttt gagtctacat aaatcaaaac aagccattac
     1741 acagttttct ttttcattac tgataatata attttgaaca tgttttagga agttctcatc
     1801 tttagtaata tttataaact tatctgaatt atttatcaat cctaaattaa ttttatcctc
     1861 atacaactta attttaagat atgaaataaa tacatttata ctaaacaaag ttaataaaat
     1921 taaaattaaa actttaatat ctacctcaat atgtcttgta agtaaaacta ttcctaaaat
     1981 tccaattata ataaaaatta ttttatttaa actgtaaatt tttgtcatgc tatttttatt
     2041 catataaatt cccctatttt ttaaattatc ttacagttaa actttatata tactaattat
     2101 aaagcacaat tgatagcaaa tcaatgc
//
LOCUS       DAEPOZ010000366         2122 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.369, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000366 DAEPOZ010000000
VERSION     DAEPOZ010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.369"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..449)
                     /locus_tag="KR440_003605"
     CDS             complement(<1..449)
                     /locus_tag="KR440_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HBH0700603.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEYLKNIFYNLFEDINIIQHNGFFIVNSKYNLDINV
                     TTQNIIETETYQSTYIFYLGELDSKADFYFRLQLCSDLLPHIIKDNA"
     gene            complement(479..1588)
                     /gene="ugpC"
                     /locus_tag="KR440_003606"
     CDS             complement(479..1588)
                     /gene="ugpC"
                     /locus_tag="KR440_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBH0700604.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNDIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRKLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDKIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
     gene            1847..>2122
                     /locus_tag="KR440_003607"
     CDS             1847..>2122
                     /locus_tag="KR440_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBH0700605.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMF"
BASE COUNT          744 a          276 c          262 g          840 t
ORIGIN      
        1 tcagcattgt cttttataat atgaggtagt aaatcagaac ataactgtaa tctaaaataa
       61 aaatcagctt tactatctaa ttcaccaaga taaaaaatat aagtactttg ataagtttca
      121 gtttcaatta tattttgtgt agttacatta atatctaaat tatatttaga atttactata
      181 aaaaaaccat tgtgttgtat tatatttata tcttcaaata aattataaaa aatatttttt
      241 aggtattcaa agtctatatg agattggtaa attaaataaa agttgccttt taaattatca
      301 agattatttt tatctagaaa atcaataaca taaatagcat tattgaaagt aactaaatgg
      361 ctactagcat ctaacttttg gttttcatag ggatagatat ttattttttt attaagctgt
      421 ctttctaaaa aatcttttaa ctcttccata aagtctcctt aaaaataaga ttatattctt
      481 atcttatagc taaagtagtt tctttgtcaa atatgtgtac tctactattg tcaactgaaa
      541 gtgttaagtc atcaccaaca ttaattctgt ttttactgtc aaatctaatt gttatcgtat
      601 tttcgcctag ttttaagtga gcataagttt ctgctcccat aagttctttt atttcaattc
      661 ttgatttaaa acaagtatct ggattagatt taataaaagt atcttctaca tgaatatctt
      721 ctggtctgat acctataaca acttcattat taatataatt gtcttttatt aaatcatagt
      781 gtttttcttt attcaattta agcttaatat gttcattttg tgcataaatt ttatcatttt
      841 cttctattaa tattacatca ataaaattca tctgtggtgc tccaataaat cctgcaacaa
      901 atatattagc tggataatcg tatacatctt gtggagttgc aatctgctga actactccat
      961 ccttcattac tacgattcta tcagccatag tcatagcttc aacttggtcg tgtgttacat
     1021 agataaatgt agttccaagc tgttggtgta gcttagttat ctctgttctc atagcagttc
     1081 taagttttgc atctaagttg gatagaggtt catccattaa gaatacctta ggatttctaa
     1141 ctattgcacg tcctaaggca accctttgtc tttgtcctcc agataatgtt ttaggttttt
     1201 tatttaaaag aggtagtaag tctaatattt tagcagcttc tttaactttt ttgtctattt
     1261 catctttagg caattttcta agtttaagtg caaatgccat gttttcgtaa acagataagt
     1321 ggggatataa agcatagttt tgaaaaacca tagctatatc tctatctttt ggttctatat
     1381 cattgacaag tttatctcca atgtatagtt ctccttcaga gatttcttca agaccagcaa
     1441 tcattctaag tgtagtcgat ttaccacaac cagatggtcc tactaaaacg ataaattctt
     1501 tatctttaat gtctatattt atatttttta ctgcgttaaa tccatttgag taaagttttg
     1561 atatattttt taaaattact tctgacataa tattctcctt gatatttaaa ttttaatgat
     1621 atagtatcaa atttttttat tatattaaat gtgtaaaatg actaaatgta tttgtttaat
     1681 atgtatattt aatttttagc aaagaataaa actaacagta aaatatattt aaaaaattaa
     1741 aaaacaaaat taaatttatt atcaaaaaat cattttatga tatgttttat tttttatatt
     1801 acaaatagag aataatgaat tatactagaa agtaggaggt gaaaatatga gcagaatttc
     1861 aatttttatg gagtcatgta atggcaaatt tccaaatgaa aattcaatgt tgtcagtttc
     1921 ctttataaaa aatattttaa atgcagaaaa caaagatttt gctaaaaata tatttaacta
     1981 tggggaagaa agaaaaaaca ataagcaaat aaaggatata aatacagcca tctacatacc
     2041 aaatcttata agaagcaaaa atgagttgct tgttgatgga gatataatat tcaatataag
     2101 tttttataat tattctatgt tc
//
LOCUS       DAEPOZ010000367         2070 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.370, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000367 DAEPOZ010000000
VERSION     DAEPOZ010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.370"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..1123
                     /locus_tag="KR440_003608"
     CDS             <1..1123
                     /locus_tag="KR440_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HBH0700606.1"
                     /translation="DAIFGTGLKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNG
                     NVLGTCIRANKTISFEFYKRGFLNYDTSTLIGDVVVEKIGVPDFITRKYHDEEFITDK
                     TFVKNNIRRRDKHGFKSDFGKVSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDV
                     QEKVSIRLTEAMTLNFEEERLNKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPI
                     VLDADGLNVMKDRCYKFLEWKNRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQK
                     YNLVVLLKGYQTVITDGKKTYINPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATV
                     SGAYIHGYIGDKLSKAMYTVNATDLISNISLTMKELLDI"
     gene            1760..1867
                     /locus_tag="KR440_003609"
     CDS             1760..1867
                     /locus_tag="KR440_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH0700607.1"
                     /translation="MGRHLWNPSYFVATVSENTEEQIRKYINNQKKQVK"
     gene            1869..>2070
                     /locus_tag="KR440_003610"
     CDS             1869..>2070
                     /locus_tag="KR440_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0700608.1"
                     /translation="MAVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIEVY
                     KNDKETFTYKQCSSDLTNLKKEL"
BASE COUNT          812 a          221 c          418 g          619 t
ORIGIN      
        1 agatgctata tttgggactg gattaaaacg tgatataact ggtattttta aagaagttat
       61 tgacatagta aacgataata gcaaaaatac atattctata gatattcctt caggtataga
      121 tagcgataat ggaaatgtat taggaacttg tattagagca aacaaaacga ttagctttga
      181 gttttataaa agaggttttc taaactatga cactagcaca cttataggtg atgtagttgt
      241 agaaaagata ggtgttccag attttattac aagaaaatac catgatgaag agttcataac
      301 tgataagact tttgtaaaaa ataatattag aagaagagat aagcatggtt ttaaaagtga
      361 ttttggaaaa gtatctatag ttgcaggttc aaaaggattt tatggtgcaa gtttcatagc
      421 aactgaatct gctgttaaga gtgggagtgg acttgtaaca ttaattagta gtgaagatgt
      481 gcaagaaaag gtatctatta gacttacaga agcaatgact ttaaattttg aagaagaaag
      541 attaaataaa ttacttaatt ctagtgatgc aattggtttt ggtccaggta tgggagataa
      601 tagtcaaaca tttgataaat tacttaagat agtagaaaat tctaattgcc caatagtatt
      661 agatgcagat ggattgaacg ttatgaagga cagatgttat aagtttttag aatggaaaaa
      721 tagatttgta attacacctc atttgggaga aatggccagg ttgacaggag attcaattgg
      781 gtatataaga gaacatagag tagatgttgc aaaagaattt gctcaaaaat ataatctggt
      841 ggtgctttta aaaggttatc aaactgtcat aacagatggt aaaaaaacat atataaatcc
      901 tacaggaaat agctgtatgg caactggtgg aatgggtgat tgtttgcttg gaatgattac
      961 ttcatttata ggtcaaggta tggatatatt ggaagctaca gttagtggag catatataca
     1021 tggctatata ggagataaat tgagcaaagc aatgtatacg gtaaatgcta cggatttaat
     1081 aagtaacatt tcattaacaa tgaaagagtt gttggatata taattgatta aaaaatagct
     1141 acgcacactt gtgactttag tcatgagtta gtagctaaaa tagtcagcat atagggaaac
     1201 ctatatgtag agataggata gaacctatcc aatactactt gaattgctaa aatcccctaa
     1261 agctaactaa actaaaacat aaggatgaaa taaacctaag tgtgaaagtt acgaaagtag
     1321 aaaaaattgg ttagatggta taaggttaaa tcctaagtac tttaaaatgg gcaatcagca
     1381 ggtaagctcc gaataggaga aacttcaacg actattcctc ttgagggaag tacactacaa
     1441 gttatcggta gtggaagtgg atagacctta gcggataatg ccgaaggata agatatagtc
     1501 tgtgcttaca tgaaagtgta agaagttcat aagagaactg cataggtagt agcgaactta
     1561 tgtgaacgac aacctcaaaa atgataaaag ttaagactta cggttcttaa atatgttatt
     1621 taaaaataaa tataaaaaac cttcaaaata cttgttttaa gtaattgcat attttaatat
     1681 tatgataggt gatgaaaaat ggaagttact catggtagag gatatgcata taatatgtca
     1741 aatatagaca tccagtatta tggggagaca tctttggaat cctagttatt tcgttgccac
     1801 tgtatcagaa aatacagagg agcagataag aaaatatatt aataatcaga aaaagcaagt
     1861 aaagtgaggt ggcagtagtg gaaaaagctt ataagtttag aatgtatcca aataaaaaac
     1921 agcaagagtt aattaacaaa acttttggat gttgtagatt tgtatataat aagtatcttg
     1981 ctaaaagaat agaagtttat aaaaacgata aagaaacttt tacttataaa cagtgtagtt
     2041 ctgatttgac caatttaaaa aaagaattaa
//
LOCUS       DAEPOZ010000368         2027 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.371, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000368 DAEPOZ010000000
VERSION     DAEPOZ010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.371"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(211..372)
                     /locus_tag="KR440_003611"
     CDS             complement(211..372)
                     /locus_tag="KR440_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700609.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            complement(715..1875)
                     /locus_tag="KR440_003612"
     CDS             complement(715..1875)
                     /locus_tag="KR440_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBH0700610.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALVVTQILHMLA"
BASE COUNT          743 a          365 c          196 g          723 t
ORIGIN      
        1 gtaggttact attttcataa taatatcacc tcttatgcaa tataatactt actaaaattt
       61 aaacttattt acttttcact taagaccatt atttatctta taaaattgag tacttataaa
      121 ctttatcaca aaaaaaccta acttaagtat ttatattcta cataagttag gttttataaa
      181 ctattataat atttattatt tcattcattt ctaatgatga ttctcctttt ttatcttttt
      241 caatttgcct tcactaacaa caaataaaaa tcccatcttc ttgtatattt catccaattc
      301 aattaaagtt acttcaccat tttttaaatt tgttaatgtt atatcttgta ttcttcttga
      361 tggaattatc ataagttatc aacactcctt taaattcttt ttaatcttta tatttaaata
      421 tatttatgca ttctgatata tattcataaa ttattaaaat aaattcaaaa aacacccgtt
      481 ctgcaaacat tcgttctgta tatttattat acatcataca gacgttcgat tcaatataaa
      541 aattaaatat atctcgtttt tagtaaattt tacaatatta tacactaaat atgtcaaaac
      601 atcttcttga aatagattat cttgtcaaac aaaaaaaacc atttcagtaa aagcttctgc
      661 ttaaattgaa atgacttttc ttatttaaat tattaatttt ataccatatt tttattaagc
      721 taacatatgt aatatttgag ttaccacaag agcacaataa gtccctataa tatttcctag
      781 aagccccata agtacaccta ctggtattaa tacaggattg tgaaccccag caatcattgg
      841 agctgtagtt actcctccta tattagctat acttcctact tcacaagtaa ataaatcaag
      901 tttaaatagt ttagcaagcc ctataaatga aatagcatgt acaaggacta ctaccaatcc
      961 aaacattatg taaacaggtg cttctgacaa tccaaagaaa tttgctttac tacctgtagc
     1021 tgcaaggagt aaatacataa acacatttcc taccagtgta ctccctttaa gctttgatat
     1081 ttttgtcata cccaataata ttgatactgt agatgcaaca agtactaccc acatagtttc
     1141 attaagtata cctgaagttg gaagtatctt ggatacaatt tgtgcaaaag acgcaactcc
     1201 aaacccaaca gaaagtaaaa tcataagttc tacaaacgta ggtgccgtat tatctttatt
     1261 ttctttttca agttttgatg taacctcatc tataaaactt gtatcgcact ttgtccattt
     1321 gttaaattta ggtccaaatt tcacagacat tagacaaaca gacatccata ctgtatatcc
     1381 tatagaaccc ataagtattg cataactaag tccttcttct gcaactccta gagcattagc
     1441 tgctgcaatc atatttgatg agccacctat ccatgaacca gatgctgccc caagaatttt
     1501 ccatgaatct gcacccattg aatttttaaa aattatgaat gctataacaa atccaagcat
     1561 aaatgtaaat gtagcacaga aaaatgataa taacatttta ggccccatct ttaatatttt
     1621 tcttaaatca cattgaatta acaagaacac taacattaat ggaaggaagt tcactgttat
     1681 tatgttttga tacgttccaa catcttcatt ctgtgcaaat aaaccaaatg atccaagtat
     1741 agctgaacct aagtatatta aaagcattgg attaataaca tcaaatacct tccatttcaa
     1801 tttatcaacc ataaaaaaaa ttacacctac gaatacaacc aagaaactac aatatacaaa
     1861 tccagtctca atcacattat acacaccctt tcttttccta attaatttga tttttatgat
     1921 ttctaagtaa agactttttc agataataga caattaaaat aaatactttt atctatatca
     1981 tgctaattaa atacatatat acctaactat tgacatatat taataaa
//
LOCUS       DAEPOZ010000369         2002 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.372, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000369 DAEPOZ010000000
VERSION     DAEPOZ010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.372"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(210..600)
                     /locus_tag="KR440_003613"
                     /pseudo
     CDS             complement(210..600)
                     /locus_tag="KR440_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     gene            1044..1613
                     /locus_tag="KR440_003614"
     CDS             1044..1613
                     /locus_tag="KR440_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700611.1"
                     /translation="MKLRNPKIKKLLSVALLAALIGIPIGQTANAMEISNNVPSYIEN
                     NPTTIEDLEINDSQLPKPGTVTYIDINKLIKNLKENGIDTTLIENELYYRKAGTNKIV
                     WTKTGFDLYLSKGVALTLAGAGTGVAGLLAGAIPGIGQYLAAAIVGAITMHLGSNITS
                     GKIFKFKKMRYNKGGAYVTKYYLQSVRNQ"
BASE COUNT          716 a          267 c          237 g          782 t
ORIGIN      
        1 tatctttatc atatttacat gatttaagct attgtagtat ttcatatatc tttaaatttt
       61 aattggtatt attctctata ttttttacta tcaaacacta aggaaataat cacttttctt
      121 tatataatgt tttaccatca cataaaacta acattaaatc caaacgctac tctcaattgt
      181 ctcaaactaa aaagatactg atagctttat tagtcatcaa tatcttcttc tttcaaattt
      241 ttaaagtatt cttctaattt gtccaagctc ttactatcta aaacttcatc atcatgtagt
      301 gctgaaataa aactcttaaa tgaattgtta tgcataaaac taaaaaaact ctttgtctca
      361 acttttaagt aatctttctc ttttatagct atagtataat gcgtacatct atctacctta
      421 ataagtagtt aaaaatccct tatctactaa agtttttaaa aatgtcaatg ttgtgctctt
      481 tttccaatcg tatttcaact tcattgcttc tatgatatct cttgaggcta atacatcatc
      541 tgctccccaa atatacttca ttagttttag ttctgcatgt ggtaatttat taatcttcat
      601 tagtttacac ctcttaccaa tttatgtata attaaatttt atactaataa tagtatgtaa
      661 tcaatcagat tggaacaaat ataatattcc aatttattcc atataaaaat aattaataaa
      721 aaagaagtac tctactttag agagtacttt tggattatat attcaaaaat ttatctaaca
      781 tgattagagt atgttttatg ctttagtgtg ataattttag ttcgttttat tattataata
      841 tgtattatat ttcattataa tctataagct taaatacagg atatttattt catatactta
      901 atttatatta agttttatta tatgtattct taagttttac tatatttttt attaatattt
      961 aatgtagtta attcttaaga attatattct acaataataa tatatatctt tgatatataa
     1021 ctaatttaaa atggaggtat tttatgaaac taagaaatcc aaagattaag aaattgttat
     1081 cagtagcatt attagcagct cttataggta tacctattgg acaaacagcc aatgcaatgg
     1141 aaatttcaaa taacgtccca agttatatag aaaacaatcc aacaacaata gaagacttgg
     1201 aaatcaatga ctctcaatta cctaaaccag gtacagttac atacatagat attaataagc
     1261 taattaaaaa ccttaaggaa aatggtatcg acactactct tattgaaaat gaattatatt
     1321 atagaaaagc tggaacaaat aaaatagttt ggactaagac aggctttgat ttatatcttt
     1381 ctaaaggagt tgcattaact ttagctggtg ctggtacagg ggttgcaggg cttttggctg
     1441 gtgccattcc tggtatagga cagtatcttg cagctgctat agttggagca attacaatgc
     1501 atcttggttc aaatataaca tctggaaaaa tctttaaatt taaaaaaatg cgttataata
     1561 aaggtggggc ttatgtaacc aaatattact tacaatctgt aagaaatcaa taggtgataa
     1621 aatgattata aaatatctac tttttgcaat tatctcagca ctagttttat tcttttgtaa
     1681 taaagtattt caactaagta aattacacaa gttcattata ttgtttgttt cattttttat
     1741 actaactttt gtagcatcat ttattaatag ataaaaaata agatggtggg gttaacctac
     1801 catctttttc tcatttaata tttgtaatat tttagatact ctttatactt gtgttactga
     1861 tatactttga ttaagaaaag taaatttaac aggaggtatc tgctatggat tttggaaata
     1921 tcaatcttat acttattgga ataatagtta taataggaac tacaataata tatttaatta
     1981 aacctaaaac tgctttttgt ag
//
LOCUS       DAEPOZ010000370         1997 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.373, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000370 DAEPOZ010000000
VERSION     DAEPOZ010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.373"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(20..1237)
                     /locus_tag="KR440_003615"
     CDS             complement(20..1237)
                     /locus_tag="KR440_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HBH0700612.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTADLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            complement(1428..1841)
                     /locus_tag="KR440_003616"
     CDS             complement(1428..1841)
                     /locus_tag="KR440_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0700613.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKTEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYYRKIL"
BASE COUNT          683 a          328 c          224 g          762 t
ORIGIN      
        1 atacttcctt atataaaaat tattttaaat caagattatc tagtattttt aaaaatattt
       61 ttacagcagt atccaagtca tctacagaaa cactttcatt aggattatga cttacgcccc
      121 cagcacatct tataaatacc ataccaatat cagttatatt atccatttcc tgagcatcat
      181 ggccagcacc actataaata taaaaaggat taagatttaa atctataaaa cttttttcta
      241 tgatttttgt tattttatta gaacatggta caggaacatt ttcaaaagca agttcacttg
      301 tataattaag ttttctattt tcacatatat gtgatatttc attaaatatc ttttctacag
      361 aattatcaag aatttcttgt gaacaagagc gtacatcgag tgtaaattca gctctttcag
      421 gtaccacatt tgatgaagat ggatagaaat tcatctttcc tacagtagca accaaatcag
      481 cagtcgtttt agctactttt tcgatagcta atacacattc acaggcacaa catccagcat
      541 caagtctcat attcattgga agtgtaccag catgtccaga cttaccattt actgatatct
      601 taaaactttt aaatcctgta atacttgata caattccagc aggaagagat tcattttcta
      661 aaacaggacc ctgctcgata tgaaattcta aatatgcatc tacatctttt ctaggatgca
      721 aatttgttaa gttttttgta ttaaatccaa actttgttac agcttcttca agtgtaattc
      781 cattttcatc aacagattta agaagttcct ctttaaaagt accagcaaaa actttacttc
      841 ccattaaaga agtttcaaat ctagttcctt cctcttcagc gaaagcaatt agcttaatat
      901 tgaaaggata agacctatta ttgtgttttg cttcttcaag accaactaga ggcactatta
      961 tacctaacat accatcaaat atacctccac actttacact atcttgatga gatgcaacta
     1021 ctaatgtggg agcatttgga aagcttgatt tatatgtacc tataacgttt ccaatgtcat
     1081 caatttcaat atcaagattt aaaccttcca tccatgaact aattaaatca atagattttt
     1141 tatgttcttg agtcaagata agtcttgtaa gaccatttgt tgttgaactt atttttccta
     1201 atgtttgtag ataatccata catttttttc ctaacatatt tacctcctaa aaatataatt
     1261 tttatttaag ctaagattag cttttatact tataattttt gaactttcaa ataattttgt
     1321 atgtatagta aaatactatt ttagtattaa taatgatata aaaacataaa gtctgtagac
     1381 gaagagcaga agttttaagt taaaaatata aaatatgatt tttattatta tagtattttt
     1441 ctataataat atctctttcc agttagagga taattttcaa gtgaaaatac tttatggtat
     1501 ccaaatttct cataaaattt aggtgcttga aaactgaatg tatctaaaaa aacatattta
     1561 caacctcgtt ctttagcaat tttttcagtc ttatataaaa gttgacttcc agtaccttta
     1621 tatctaagct tctcactaac ccataagtat tttatagaaa gccaattacc atgagtaaca
     1681 cctataaaac ctgcaacaat tttacctgct ttatctcgaa tatatattcc taaatccact
     1741 gggtttttat cttcaagtct agcaagatta tattttaata atccttgaaa aatagtatct
     1801 tcatcttgtt tttctatttt ttctgtaatt tctaattcca tatttgaact ttctccttgt
     1861 ataatctttt gtatattggt attttttaat aaaatcagta gaaagaatat agtttaacat
     1921 tattctaata ggcatttatg ctaatgtcaa actaataata cttattctta attttatatt
     1981 gttgatatta aattttt
//
LOCUS       DAEPOZ010000371         1992 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.374, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000371 DAEPOZ010000000
VERSION     DAEPOZ010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.374"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(301..1629)
                     /locus_tag="KR440_003617"
     CDS             complement(301..1629)
                     /locus_tag="KR440_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBH0700614.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGDKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIVIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGIKR"
BASE COUNT          724 a          348 c          219 g          701 t
ORIGIN      
        1 aaagatttaa ttttcataaa acctatgtat aatatttgtt atatagatat cacttagttt
       61 gaaaaaaaaa cactttaaaa taaagtatct taaagcaaat aattttttag atgtttaatt
      121 ttataattca aaaactaaaa ataaagtgaa taccttaaaa taaacttttt agttcataga
      181 acactctttt tttataaaat aaaacatctc tttacccttt taacaataaa aaagagactt
      241 atctctattt tgagacagtc tcttttattt taattataaa gcatttgtaa actacttggt
      301 ttatcttttt attcctctag tcatataagc atacactggt aagcctatag ctgttattac
      361 aagacctcct atagatatca tagtagtttt catccctgca aaacacaatt gattaactac
      421 aacaaatata ccacttgcta tagctattat aggaacaata ggatataatg gaactttata
      481 ctctcttggt atatttggat gagtctttct aagtttcata acccctataa aagttaatac
      541 atagaatatc caagtagcaa atacagctaa gtcagttaat aaattaaatt gacctgacaa
      601 agcatatata gatgcaataa cagatactaa tgctattgca tttgctggaa cttgagcaga
      661 atttaattta gaaaatatac catactttgg taaagtctta tctgtagcta aagtatatgc
      721 cacccttgaa ccagttagta aaaatccatt taatgctcca aacactgaaa ttaatatacc
      781 tacagatatt atttttccac ccattgaacc aaatataact tcagcaactg ctgatgcagg
      841 agaagcataa tttgctaatt cattagctgg taatacccaa agatacgcta aatttattat
      901 aaaataaaca gccataacaa ctgacaatcc acctattata gcttttggaa ggtcttttcc
      961 aggattcttc atttctccag ctaaagtacc aacattcatc catccatcaa aagcaaataa
     1021 tatagcaatt aaaacttgac ctagtacact acccaggctt aatccttcac caaccatagg
     1081 tgtaagtatt ggattatttc caccaccttt aataaatcca acaaccatta ttaaaataag
     1141 tggtattaac ttacaaattg tagagacagt ttgtatgact cctccagttt ttgaacctaa
     1201 catattaagc cctgcaacta ttactataat tccaattgct attggaagta aaagtgatgg
     1261 gctaccaatt agtgctgatg cttgttctcc aaatataaca cctaatgctg ccatcatccc
     1321 tggataaaat aatacaattt gcatccatcc agttaaaaaa cctaatttgt ctccataaat
     1381 ttcttttata tataccatca ttccaccagt ttttggtatc gcaactgata cttctgcagc
     1441 tgtaagacca gctgttatag ttatgattcc agctattaac catgccaata taccaagacc
     1501 tggagctcct ccagttaatg tatagactgc ttgtggttta aaaaatacgc ccgctcctat
     1561 aacagaacca acaacagttg ataatgctgc tgatacgcca agagtctttt tcagttcatt
     1621 acttcccatt cttttcctcc tcattaatcc tatttaattt taatttcata gttaaataag
     1681 catatattat gccaatatat agaaaaatag cttttttatt tattaaaata aaataaactc
     1741 tacttagctt tcgtaatctt ttacttttag ttatgtttaa atataaaaaa attataaaaa
     1801 ccaaaaatcc aaatataata atatttataa ataaacgtat tttttatatt gtataatata
     1861 cattgtatat ttgtaacact aaaaatagat tataactatt aagttaaatt gattttgatt
     1921 tttttgcaat aaaatttcat ttttttcaaa aacattttgg cttctttctg taattttcta
     1981 tctttaaggt ct
//
LOCUS       DAEPOZ010000372         1983 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.375, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000372 DAEPOZ010000000
VERSION     DAEPOZ010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.375"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            178..294
                     /gene="rrf"
                     /locus_tag="KR440_003618"
     rRNA            178..294
                     /gene="rrf"
                     /locus_tag="KR440_003618"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            301..375
                     /locus_tag="KR440_003619"
     tRNA            301..375
                     /locus_tag="KR440_003619"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:333..335,aa:Asn,seq:gtt)
     gene            380..454
                     /locus_tag="KR440_003620"
     tRNA            380..454
                     /locus_tag="KR440_003620"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:413..415,aa:Glu,seq:ttc)
     gene            460..535
                     /locus_tag="KR440_003621"
     tRNA            460..535
                     /locus_tag="KR440_003621"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:493..495,aa:Val,seq:tac)
     gene            541..617
                     /locus_tag="KR440_003622"
     tRNA            541..617
                     /locus_tag="KR440_003622"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:575..577,aa:Asp,seq:gtc)
     gene            628..702
                     /locus_tag="KR440_003623"
     tRNA            628..702
                     /locus_tag="KR440_003623"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:660..662,aa:Thr,seq:tgt)
     gene            717..801
                     /locus_tag="KR440_003624"
     tRNA            717..801
                     /locus_tag="KR440_003624"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:751..753,aa:Tyr,seq:gta)
     gene            811..884
                     /locus_tag="KR440_003625"
     tRNA            811..884
                     /locus_tag="KR440_003625"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:843..845,aa:Gly,seq:tcc)
     gene            895..971
                     /locus_tag="KR440_003626"
     tRNA            895..971
                     /locus_tag="KR440_003626"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:929..931,aa:Arg,seq:tct)
     gene            981..1056
                     /locus_tag="KR440_003627"
     tRNA            981..1056
                     /locus_tag="KR440_003627"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1014..1016,aa:Gln,seq:ttg)
     gene            1068..1143
                     /locus_tag="KR440_003628"
     tRNA            1068..1143
                     /locus_tag="KR440_003628"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1101..1103,aa:Lys,seq:ttt)
     gene            1146..1234
                     /locus_tag="KR440_003629"
     tRNA            1146..1234
                     /locus_tag="KR440_003629"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1180..1182,aa:Ser,seq:tga)
     gene            1252..1342
                     /locus_tag="KR440_003630"
     tRNA            1252..1342
                     /locus_tag="KR440_003630"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1286..1288,aa:Ser,seq:gct)
     gene            1351..1427
                     /locus_tag="KR440_003631"
     tRNA            1351..1427
                     /locus_tag="KR440_003631"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1385..1387,aa:Pro,seq:tgg)
     gene            1513..1588
                     /locus_tag="KR440_003632"
     tRNA            1513..1588
                     /locus_tag="KR440_003632"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1546..1548,aa:Trp,seq:cca)
     gene            1649..1725
                     /locus_tag="KR440_003633"
     tRNA            1649..1725
                     /locus_tag="KR440_003633"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1683..1685,aa:Pro,seq:tgg)
     gene            1732..1808
                     /locus_tag="KR440_003634"
     tRNA            1732..1808
                     /locus_tag="KR440_003634"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1766..1768,aa:Ile,seq:gat)
     gene            1812..1887
                     /locus_tag="KR440_003635"
     tRNA            1812..1887
                     /locus_tag="KR440_003635"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1845..1847,aa:Phe,seq:gaa)
     gene            1895..1971
                     /locus_tag="KR440_003636"
     tRNA            1895..1971
                     /locus_tag="KR440_003636"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1929..1931,aa:Met,seq:cat)
BASE COUNT          501 a          449 c          535 g          498 t
ORIGIN      
        1 aatgtattta gcttatcgat actaataggt cgaggacttg accaatatta tttgatgtta
       61 ttcattaaga tatatgtgca gtttttagag tgttaactct aaaaaaaaat aaaaaaagta
      121 ttgacaaaaa tagtaaaaaa tgttaatatt atacttgtcc tagcaacaag aaaaaaatgt
      181 ggttataata gcagagaaga tacacctgtt tccattccga acacagaagt taagttctct
      241 agcgctgatg gtacttggtg ggaaactgcc tgggagagta gggcatagcc acgtgaatgt
      301 tccaaggtag ctcaacggtg gagcaaccgg ctgttaaccg gtaggctgtg ggttcgagcc
      361 ccacccttgg agccattatg gctcgttggt caagaggtta agacaccgcc ctttcacggc
      421 ggtaacaggg gttcaattcc cctacgagtc accatttgtg ggatcatagc tcagctggga
      481 gagcacctgc cttacaagca gggggtcaca ggttcgagcc ctgttggtcc caccatttgc
      541 ggcctggtag ttcagttggt tagaatgcca gcctgtcacg ctggaggtcg agggttcgag
      601 ccccttccag gtcgccaaaa taaaaatgct ggtgtggctc aacggtagag cagctgactt
      661 gtaatcagca ggttgtaggt tcgattccta tcaccagctc catgtaaaac taatacggag
      721 gatttcccga gcggccaaag ggggcagact gtaaatctgt tgtcatcgac ttcggtggtt
      781 cgaatccacc atcctccacc aattaaatat gcgggtgtag ctcaatggta gagttctggc
      841 cttccaagcc agctgtgagg gttcgatccc cttcacccgc tccaaagaaa gcgtgggtgc
      901 atagctcagc tggatagagc aacgcccttc taaggcgtgt gtccggggtt cgaatccctg
      961 tgcgctcacc acatatatat tggggattag ccaagtcggt aaggcacatg actttgactc
     1021 atgtatgcgt aggttcgagt cctgcatccc cagccaaata aaaatatggt ctattagctc
     1081 agtcggtaga gcacctgact tttaatcagg gtgtcccgcg ttcgagtcgc ggatagatca
     1141 ccaatggaga ggtgtccgag tggtttaagg agctggtctt gaaaaccagt gacttcgaaa
     1201 ggggccgtgg gttcgaatcc caccctctcc gccaaaaaaa tgactgaatt tggagaagta
     1261 ctcaagtggc tcaagaggat cccctgctaa gggattaggc tgggtaaccg gtgcgagggt
     1321 tcgaatccct ccttctccgc cattcaaaaa cgaggtgtag cgcagtttgg tagcgcacat
     1381 ggtttgggac catggggccg ggggttcgag tcccttcacc tcgaccaact taagctttta
     1441 gttcagttgg ttagagtgcc caactcatgg ctagtaattc tagggtttga gtccatgaag
     1501 gttattcttc ataggggtgt agctcagttg gtagagcgtc ggtctccaaa accgtgcgcc
     1561 gggggttcga gtccctccac ccctgccaaa taaaaaggac ttgaaaaaag ttggatttta
     1621 gagtagtata agatagtaaa taagatttcg aggtgtagcg cagtttggta gcgcacatgg
     1681 tttgggacca tggggccggg ggttcgagtc ccttcacctc gaccaagaaa agggcctata
     1741 gctcaggtgg ttagagcgca cgcctgataa gcgtgaggtc gctggttcga gtccagctag
     1801 gcccaccatt tgcccagata gctcagtcgg tagagcaggg gactgaaaat ccccgtgtcg
     1861 gtggttcgat tccgcctctg ggcaccagga atacggcggt atagcttagt tggctagagc
     1921 gttcggttca tacccgaaag gtcataggtt cgactcctat taccgctacc atctaactat
     1981 atc
//
LOCUS       DAEPOZ010000373         1842 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.376, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000373 DAEPOZ010000000
VERSION     DAEPOZ010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.376"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(281..1195)
                     /locus_tag="KR440_003637"
     CDS             complement(281..1195)
                     /locus_tag="KR440_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HBH0700615.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKDITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
BASE COUNT          653 a          222 c          234 g          733 t
ORIGIN      
        1 attacgaaaa tagttacaat atagttataa cataaatatg cgattagtat aagatttact
       61 attagttttt aacaaataac tgaataaaga aatacttata aaacataatc actaatactt
      121 tttatttaat ataaaataaa gttgtgggtt atagatgatg attattttat tttatgaata
      181 acttgtttaa attttataag tttataaaaa ataaaggtac agttaactgt acctttattt
      241 tatctaaata tgcaaagatt attttaaaag taatttttaa ttacatgttt ttataactct
      301 ccaatattct aatataatga tttgcctctc ttaaaacatg gtctgctaaa agtggtaata
      361 ttatagattt tatcttacac tgttctattc ctgatgctcc tgcttcttta aaatctttaa
      421 actcaacagt ttcatttaga gtttcttctg taatgttctt gatattagaa tcagtcattt
      481 cgtttgtttt ttcaattaat tcattaaatt ttatagcaaa atcatttgaa gtatttatta
      541 gttcaccttc tgaggggtct agtaatcctc tcataaacag agcatgttcc atcataatat
      601 ggtcccagaa taattctatt tctttagcgt tttttgactc aatatctatt ttattttcaa
      661 ggtcaactac ataagaacga tataaatttg cttcatgtat tatatgttca agaagtagag
      721 ggtagtttga tgtaaatata gtacatgata gtacaccatc taagactctt tctttaaaat
      781 taataagccc atcaagtaat cttattgcat cagagttaag ttgagctaca tagttcacta
      841 agtcttgacc aacttgtggg tttacaccac tttgtagatt taattctctt gtagtgatgt
      901 ctttgtttat ttctatccct gtaaactctt ctgttttttg ttctgcaact gatgtgagag
      961 tagttacaag ctcttctgaa tataatatat caggtctaat tataccatta ctagcactaa
     1021 cagtgtatga taataaatct tcaaattgct ttttatagtg gtcagcttcc atagcaagat
     1081 tataattttt atttgtgaat cctgcttcta aaaaaagagc atgctctttc ataattcttg
     1141 aaaaaaataa atgtaattct agtgataaaa taacatattt ttgattatct atcataattt
     1201 cctcccttta gaaagtatcc tatttataat actttattcg taatatttaa atttatgtat
     1261 attataaata aattaactaa attcaattgc tactaatatc aacagttttt attaagttat
     1321 gggcgaaaaa attatcttac tgtgtttctt attagatata gatataatga tttaaggtac
     1381 ttgttggcta ttatttatta agagcacaag taatactaga aatttttatg agaattatgt
     1441 aatttataag tattattttg atacaaaaca aatatttttt gtgaaacaag aacttttata
     1501 gcaaaaaatg aataaatatg aagcaatcat aattttatac cacaacaatt attaattcta
     1561 atagctgcac ttaatgtttt aggtatggga tttaaaaagt ataaaaaatt aagtgataaa
     1621 tatattccat tatattgctt gtgcttggag tagtattttc tgtatggatg ttaggattta
     1681 atccaacttc tattttacag gggattctat gctggggtgt ttcaattgga ttaaattaag
     1741 tatgcaaaca atttcaagac aatgataaat aattggatgt gtgataggaa tatcaacaaa
     1801 aaacattatt aaaaagaatt attttattta taaaataaaa ca
//
LOCUS       DAEPOZ010000374         1824 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.377, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000374 DAEPOZ010000000
VERSION     DAEPOZ010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.377"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            148..1746
                     /locus_tag="KR440_003638"
     CDS             148..1746
                     /locus_tag="KR440_003638"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HBH0700616.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDMDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILAVTDIIKEGSYLIYKAEDSLIANAFDVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
BASE COUNT          699 a          220 c          279 g          626 t
ORIGIN      
        1 ttaaatttgt agtatttatg taagaaataa attttatata atattttttc actaaatttt
       61 tttcttttta caacaaatac tcactacctt ttgatataat atttttatca attattatta
      121 tattcaagtt ttgaaaggag tattaaaatg agtttattag ttttcggaca taaaaatcca
      181 gatacagatt caatatgttc tgcaatttca ttagcacatt taaagaacaa actaggaata
      241 aaagcaacag cttatgcttt aggagatatc agaaaagaag ctaaatatgc cttggattac
      301 tttaaggtag atgcacctga aattttagat aatgttagaa tacaagttag agatttaaat
      361 tatgataaag tagtaccttt aacaccaact tcatctatat tagaggctta taaccttatg
      421 gatgaaaaaa atgttaaaac actacctgta acttatgatg atggaacttt tgctggtatc
      481 attactatga aagaaatagc taaaaatcta cttcatcaac actttacaac tatacacaca
      541 tcactatcaa acatttgtaa aaaccttaat ggtactattc ttgtggactg tggtgaagat
      601 ataaaaggta gagtagtcac tttatcattt ggaatggaga ctctaataga gactttaaaa
      661 gaaggagaca tagctattgt tggagataga tatgatagta ttgaatatgc tattgataca
      721 aaagttaaac ttttaatact tactaatcat acagagattt caaaagattt gctagctctt
      781 gctaagataa atggagtgtc tgttgtttct gttgaaagtg acaattataa aacttctaat
      841 gtaataaatc aatgtagttt cttagactct atagaagcta cagaaaattt aattacattc
      901 aaagaagatg attatatgga tgaaataaaa gagattatgc tagaaacaaa cttcagatct
      961 tatccaatat tagatgatga taataagttt ttaggtttag tttcaaaagg tcatctatta
     1021 aacccatcta aaaagaatgt tgttttagtt gaccataacg aatatgctca aagtgctgat
     1081 ggaattgaac aagcaaacat agttgaaata attgaccatc ataaacttgg tggtatttct
     1141 actgatgttc ctatgtcatt tagagttatg cctgttggat gcaatagtac tataatttat
     1201 caaatgtaca aagaaaacaa tgttgagata ccttatgaaa tagctgggct tcttttatct
     1261 gctatattat cagatacatt attatttaag tctccaacaa caactgatat ggataagaaa
     1321 gcttgtgaag aattaagtaa aatagcaaaa gtagacatgg aaaaatacgc tatggatatg
     1381 tttaaatgcg gtacttcttt agatgaatat actatagaag aaatagtaaa tatggatttt
     1441 aaagaattta atatgagtgg acatagagtt ggtattggac aagtatttac cttagacata
     1501 gactctatat ttgctaaaaa agatgagttt ttatcttata taaattcgac tgattatgac
     1561 aaactaattc ttgcggttac agatataatc aaagaaggtt cttatttaat ctataaagca
     1621 gaagatagct taattgctaa cgcatttgat gttgaaggtg ttcaaggtac atttgcacca
     1681 ggtgttgtat caagaaaaaa acaattagtc cctaatttaa ctactgcaat caaaaatttt
     1741 aaataatata aaatttttaa ataacgaaaa ctgtctcaaa tctatatttg agacagtttt
     1801 ttattataca tatattgata ataa
//
LOCUS       DAEPOZ010000375         1819 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.378, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000375 DAEPOZ010000000
VERSION     DAEPOZ010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.378"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            24..>1819
                     /locus_tag="KR440_003639"
     CDS             24..>1819
                     /locus_tag="KR440_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBH0700617.1"
                     /translation="MFNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLAN"
BASE COUNT          750 a          169 c          359 g          541 t
ORIGIN      
        1 ttataaagga gggaaatttt aaaatgttta ataaaaataa agaaaatata actttaagta
       61 atcaaatcaa aggaacaatt caaaatagtt taacaaaaga agctatatta catataccaa
      121 tgagcaatta tgcctatggt tatgatagag aaacactaca tataagactt agaactaaaa
      181 aaaatgaagc gaaaagagta attttacgaa taggagacca atatgtgtgg gataaaggag
      241 gtgcaggagg aggaaattta aatgcatctg gagttggttg gtctggtgga acaaatatag
      301 taatgagtaa agaagtagaa actgaattat ttgactattg gatagctaag tgtaagcctt
      361 taaataaacg ctcacgatat ggttttataa tagaaggtca agaagaaaaa cttttattta
      421 cagaaaaaag aataatagag ttgggaaatg aaaatgatga aaaggaatta tgtgaaatag
      481 gtaatttttt tggatatcca tatttaaatt atatagatat acctaaagtg ccaaattggg
      541 tgaaagatac tgtttggtat cagatatttc cagatagatt tgcaaatggt gacccgtcta
      601 taaacccaga aggagtagat aaatggggag ctattccaac cagagataat tttacaggtg
      661 gagatttaca aggtgtgata gaacacttag attatttgag tgatttggga ataaacggaa
      721 tttacttttg ccctattaca attggaaaaa ccaatcatag atatgatacg gtagattata
      781 tggaggtaga ccctacatta ggagataaag agactttaaa aaaactaata gaagaagcgc
      841 ataagagaaa tataaaaata atgttggatg ctgtttttaa tcatatagga tattattcta
      901 aacagtggca agatgtagtt caaaataagg aaaattctcg atataaaaac tggttttata
      961 taaaagatat gagtaaaatt gatactccaa tagaacagat agatgaaaaa aatatacctt
     1021 atgaaacctt tgggtgtgaa aaatatatgc ctaaacttaa tacagaaaat tcagaagtaa
     1081 taaaatattt attggatgta ggcaagtact ggatacagga atttgatata gatgcgtgga
     1141 gattagatgt ttcaaatgaa gtagaccatg ttttttggag aaagtttaga gaagaagtca
     1201 aaaaagtaaa accagacatt tatatattgg gagaaatttg gcatggtagt ttaccatggc
     1261 taatgggtga ccaatttgat tcagttatga attacttgat gtcagaagcc atgaaaaaat
     1321 tcttttgtac agatgagata aacgccgaag aatttaagta tatgataaat gatgtaatgg
     1381 taagttatcc aatacaagtt agtgaagtta tttttaattt acttggaagt cacgatacta
     1441 caagaatatt gacttatgct aatggaaata ttgatagatt taaacttgca tatatgttta
     1501 tgtttgtaca aacaggatgt ccttgtatat actatggtga tgaaatcgga atggaaggag
     1561 aattaagttc aatatctgaa ggtcaaagaa aatgtatgga atggaatgaa gaaaaatgga
     1621 ataaggatat atttgatttt atgaagaaga ttattagact tcgtaaagaa aataaagaac
     1681 ttaggtctat atcaaatgaa tgggtgttag cagataagga aaatggaacg attatattga
     1741 aaaaagaatc tatttctata ataataaata attcatctaa aaatataatt ttgacgttac
     1801 cagattattt agcaaataa
//
LOCUS       DAEPOZ010000376         1799 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.379, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000376 DAEPOZ010000000
VERSION     DAEPOZ010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.379"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            117..449
                     /locus_tag="KR440_003640"
     CDS             117..449
                     /locus_tag="KR440_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBH0700618.1"
                     /translation="MNTREMKEYRGFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
     gene            991..1155
                     /locus_tag="KR440_003641"
     CDS             991..1155
                     /locus_tag="KR440_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700619.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
     gene            complement(1379..>1799)
                     /locus_tag="KR440_003642"
     CDS             complement(1379..>1799)
                     /locus_tag="KR440_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700620.1"
                     /translation="KKEFGINSMDLAQLKLNDFGLKYFKEVDLILKKAIAINPTDSKL
                     YYAQACNYMNFDGFPGYSDTTYNALDSINNSLKYLDKNDSDYKKNASNIYNLKGVILM
                     FHKRFDEAQQYLNKALYFNKDNEDPKYNLNTIKKHLN"
BASE COUNT          691 a          142 c          264 g          702 t
ORIGIN      
        1 agatttcaaa aaataatata aattaggcta atgaacataa aatgaaaatt tgtatttaat
       61 attgaaaata taagtacagt gtgatactct tataagaaaa acataggagg tagaagatga
      121 atactagaga aatgaaagaa tataggggat tttacaataa aaaattaagt gaaacagatg
      181 agttttttag agtatttgga agtcttgatg ataagactta ttcagaaggg gctataagta
      241 aaaaaaataa ggaattaatg ggactagcta tttcaatttt aagccgatgt gatgagtgta
      301 tttgttatca tattgagggt tgtattaatt ctggagcaag taaagatgaa attatagaag
      361 ctataaaaat aggtgtaatt ggtggcgggt ctattacata tccaaacgca agatttgcaa
      421 taaaaatttt agaagaattt aatatataat tttgatacac gtagtttatt tagactaata
      481 ataatattct atttaagtct ttgaaattaa gctttatttt ctaagttcac ttcttattaa
      541 gtttttagaa ttgcatatat aaatataaat ttttaaataa tatagtagtt ttaaattatt
      601 tacatcatag attgtattga gaatagtata ttgtcattat taaatctttt atacattttt
      661 tattttaaat aaattgaata ttaaggtgat tatatttaca tatttgtatt tttatgaact
      721 agaatattct ttataaattc atagcagttt taatagataa ctttttcaga tagattaaac
      781 aaagttttat ttactgtatt ctattatcag tttcctacaa attaaattgt ctatttttat
      841 ttatagagta gaataaactg tttttgttaa gaattttgaa ggtaaagcta gtttaaaaaa
      901 ataaagggac taatttgtct aacgtttatt ggtgaaaaat aagtctatag aagttataat
      961 aaatatataa aaatgtatga gaggtgttat atgtttggta aaccaaaaga aagtgttaga
     1021 tttttagact ttaatgatgt agcagaaaag tttggagaga aaaaggctgt tgcaattttt
     1081 gatgccatag ctggaaaaaa atctaaagaa catattatca aagatataga aaaattagta
     1141 aaaaatgaag tttagatatt tataaggtat attgagcttg agggtagctc tttatacctt
     1201 cttttttatg atttttagat aatattttaa aatttaaata agaaacttat tgttatagag
     1261 taatatttat atagaaataa gtctttataa tttaaacttg taattagttt agtagagatt
     1321 atgattcagt gctgaattat acataaaaaa atattttata aattcaaaaa ttaaatatct
     1381 agtttagatg tttttttatt gtatttagat tatattttgg gtcttcatta tctttgttaa
     1441 agtataaggc tttatttaaa tattgttgtg cttcatcaaa tcttttgtga aacataagaa
     1501 taactccttt taagttatat atattacttg catttttttt gtagtcagaa tcatttttat
     1561 ctaaatactt taaagaatta ttaattgaat ctaaagcatt atatgttgta tctgaataac
     1621 caggaaatcc atcaaaattc atataattac atgcttgggc ataataaagc tttgaatcag
     1681 taggattaat agcaatagct ttttttagta taaggtcaac ttctttaaag tattttagtc
     1741 caaaatcatt aagcttaagt tgagcaaggt ccatactatt aataccaaat tcttttttg
//
LOCUS       DAEPOZ010000377         1768 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.380, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000377 DAEPOZ010000000
VERSION     DAEPOZ010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.380"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(165..1457)
                     /gene="brnQ"
                     /locus_tag="KR440_003643"
     CDS             complement(165..1457)
                     /gene="brnQ"
                     /locus_tag="KR440_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBH0700621.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFALSYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVDVPVV
                     KSLPLHSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT          639 a          303 c          207 g          619 t
ORIGIN      
        1 ataaactata tatttttttg atattttctt atactttttc catattaaat acaaataatt
       61 aattatagaa tcccaaaata aaaggagtta tttgtacaaa taactccttt tacttttagt
      121 taactcattt atttacaatt gattatattt taattaaaca aaacttagtt tgttccaagt
      181 gtattagatt gactctttga tggtataaaa ttaccaatta ttccagctat tataactggt
      241 accacccagt taaatcctaa tgaatgaaga ggtaaagatt ttactactgg aacatctact
      301 ccataactag atataactgt tagtatactt acaagcaatg acatatatac tgctcctttg
      361 tacacattat cattcttaat tttttcattg aagaaagcta aaattattaa aacaattgat
      421 ggtggataaa ctatacttag tataggagat gctatcttta ttatagttcc aacaccaaaa
      481 gatgctacta ctgcactgaa tacagataca gctaatacta ttttttcata actcaattta
      541 ccttttgtaa gtctagagaa atattgacct gtggcagatg taagacctat agatgtagtt
      601 aaacatgcta atgatactac tactgctaaa atcgctttcc caacatttcc aagtaatcct
      661 tcagttatat ttactattac ttgtgattgt actgcatctg ttccatacat tgttgataca
      721 gttgctccaa ggaaacataa tcctccataa actagagcta atcctccaca agcaagaacc
      781 cccgccttac caatcatttt taacttgtca ctaatagtag aatatccttt atcatttaaa
      841 gatactatta acactgatgc aagtgctatt gctgcaaaag catccattgt ctgatatcct
      901 tgacctatac cttctgcaaa tacattaggt atcataggtt tatctactat atctccaaga
      961 ggtgatataa taccttttat aattataaat gctagagcta ttaaaagagc tggagttaaa
     1021 aactgtccaa ctatatctac gaccttagaa ggtcttattg ttagtacata tgaaagcgca
     1081 aaaaaaagta tacagaataa taacactgga attgaagttc caaataaagg cattataccc
     1141 atttcataag ttgttgcacc tgttcttggt atagctaaga atggacctat acataaaatg
     1201 tctgcacaac ctataagaat ccctaacttc ataccacatc ttttaaacat ttcaactacg
     1261 tttccatcga atttagctgt agccattact gctagaagtg ccaatccagc atcagcaaac
     1321 gtaaatccta agaaggcaat taaccattct ggacctgtaa ttattcccaa gtaaggtgga
     1381 aatattaagt tacctgctcc aaagaacatt gcaaataacg caaatccaaa tactataaca
     1441 tccttggtct tacccattat aaaatccccc tttttaacct tattaaatta ataaaatgtc
     1501 tcgattgttt acgcttgcat aaataaatta aattaaacaa taagaactta ttattaaatt
     1561 aataaaatta aataaattaa atttttaata tattgtattt atttgtgctt attatatatt
     1621 atacgaaaca agcaagtcaa tatgctaaaa tttaaaataa tcacactcat tttaattttc
     1681 aaaatattag gtgtatttgt cttaaattaa tatttttaaa ggttttctaa cctaatttat
     1741 acttttatat ggagatattt gtctatat
//
LOCUS       DAEPOZ010000378         1756 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.381, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000378 DAEPOZ010000000
VERSION     DAEPOZ010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.381"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..843
                     /locus_tag="KR440_003644"
     CDS             <1..843
                     /locus_tag="KR440_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700622.1"
                     /translation="DCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQIS
                     KSIEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNIL
                     NIKFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYER
                     SAQDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFEN
                     SLDIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGED
                     ELNE"
     gene            836..1468
                     /locus_tag="KR440_003645"
     CDS             836..1468
                     /locus_tag="KR440_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBH0700623.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSKIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
BASE COUNT          733 a          167 c          279 g          577 t
ORIGIN      
        1 gattgtgaac atgttttgat acaaataatg ttaaaatctg aaaatttact aacatctgaa
       61 ataactcatt ttaagtcaga aagaatggat ttaaaagtag aagaggtaaa aaatacaata
      121 aaacaaataa gtaagtctat agaagctatt gaaaaatttg ataaaatatc taaagattta
      181 aatgacttta atgatgaatt tatcagtggt atagaagctt taaaaagcct tttagaaggt
      241 tctcaaagtt ctattaagac atttgaccag agcgtaagaa aattagataa acagtttaat
      301 attttaaata taaaatttgt aaagcttttt gataaatatg ataatcaaga taatataaat
      361 aaagagattt tatttgatat aaaagaatct tcaaaaaata tatacaatgc cactgaaagc
      421 caattcaaaa ttagagatta tataaaaaat ataaatgcag gatttgctct atatgaaaga
      481 agtgctcaag atttattaac taaattaatg acccatgaaa ataaaatctc tcaaaatcaa
      541 aaaatccttt tagatgaaaa gtttacctta gatgactcaa taaaaaatct ctcaagtatt
      601 attgaaaact tttctaatga cctacaagtt aaactggata tgatgtttga aaattcttta
      661 gatattcaag ataagttaga tgttatgttt aataactctt ttatgaatga tgaagttcct
      721 cttgatagtg aggaattatt taaggatgat gttaataatg tatttaatcc ttttagtgaa
      781 gaaatttatg agatagagga taaagaaata aaggtcatag gtgaggatga gttaaatgaa
      841 taataaatat agaagaactg ttaacagaga atttgaaaag agtagctttt ggccaacatt
      901 tacagattta ttatctactg tattgatggt tgtgattctt atcttattta gctcagaaag
      961 tatttcaggt tctgttgaac aagatttagc taagaatgtc aatgcttctg ttgaagaaac
     1021 ttttaaaaaa agcaaaatac ctgtgaaggt agataaatct aatgggcaag taacttttgg
     1081 tgaaaaaacc atgtttgatg tagatagtga tgttttaaaa ccagaggcta aagaaatgct
     1141 aaaaatgttt gttccaaagt acatagagac tatttataaa gattatggtg actatatatc
     1201 caaaatagtt atagaaggtc atacagatga tgtgggaagt tatatataca acttagattt
     1261 atcgcaaaga agagcatata gcgttgctaa atttatagtg ggagatgaaa taggtgacta
     1321 taaatacaaa gataaagtga ctaagcatat tattgcaata ggaaggtcaa aagctgaact
     1381 catcaaaaat ggtgacaata gcgtgaatag agatgcatct agaagagtag aactaaaata
     1441 cgaaataaat attaatcaaa ataaataatt aaatttttat aaaaaaaaag aaggcagttg
     1501 gtgtgagctt taagccttct ttaaggaaaa agtttattta tttttgttga tacttaacat
     1561 attatagttt ttagaagcag tttgcaatat attaaacgta aatggtcgtt taaaatgcat
     1621 aactggatac tactgatgta aaatatttca ttttatataa tataaaaatt atacagtgtc
     1681 aattttctat attattgtac tataatctat tatttaaaat attatgtaaa aaaatatcat
     1741 aaaaacgcat atcatg
//
LOCUS       DAEPOZ010000379         1753 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.382, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000379 DAEPOZ010000000
VERSION     DAEPOZ010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.382"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            235..690
                     /gene="smpB"
                     /locus_tag="KR440_003646"
     CDS             235..690
                     /gene="smpB"
                     /locus_tag="KR440_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBH0700624.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
     gene            848..1197
                     /gene="ssrA"
                     /locus_tag="KR440_003647"
     tmRNA           848..1197
                     /gene="ssrA"
                     /locus_tag="KR440_003647"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          672 a          205 c          308 g          568 t
ORIGIN      
        1 ctggttaaag ttagtttagt attaagtata ttcgaagtag ttttttagct attttcatat
       61 ctttatatta cttaattata tatagcctaa ctaacttaat atatcagtga aatattgact
      121 aaaatgccta aatatgttat catacaagtt agtcaaataa tttggcaaca ttaaatattt
      181 agtaaatttc taacatttat ggaataaatt tctaacaaag aaggtgagat ttatatggct
      241 ggtaaaaaaa tattagcaac aaacaaaaga gcaagacatg aatattttat agaagaaact
      301 tatgaatgtg gaattgaatt aaaaggaaca gaagtaaaat caataagaca aggcaaagtt
      361 aacctaagtg atggatttgc atctgtagat aatagtgaag tatttttaaa acaagttcat
      421 ataagtccat atgagcaagg aaatatattc aatgttgatt ctttaagagt tagaaaacta
      481 ttacttcata agcatgaaat aagaaaactt attggagcaa ctactataaa ggggtattca
      541 cttataccac ttagtttgta tctaaaaaac ggtaaagtta aagtagagat tgccttagca
      601 aaaggtaaaa aactatatga taaacgtcaa gatatggcta agaaagatgc acaaagacgt
      661 atagaaagag aaatgacagg taagtattaa actagataaa actgtatttt ttagaataaa
      721 attctttatt atctagaaca taacattgat ttttattctt aaaagtatta tattatatat
      781 acattaaaaa tttaataaaa atggtgataa agtttgcgaa aagtctactg gactataaac
      841 tactcaaggg ggcgtaaagg tttcgacgtg ggtttggaac ttgaggctgc atgtcgtgtt
      901 actctgggtc acgtaaaaac tggggaactt taaaataaac gcagacgata atttcgcgat
      961 agcagcctag ttgctgctcg tccctcttag tcctcctgcc gactaagact ggacgtcatt
     1021 atgcagggaa ctactttagg ggtgtctcga cctgaagtag attaattggg actggctaag
     1081 catagagcct gccacttggc gctatgtgag gtgagatatt agtgaagttg gctaagcatg
     1141 tagatgcagc aaggaaaaga tttgcggaca ggggttcgac tcccctcgtc tccaccaatt
     1201 tttaaaaaag aatgatgaaa tacttggaaa tactagtctt tattgactag tgttttttgt
     1261 tttggagatg aaatccacca accgtgcccc aaccacagaa aaagagattt ttttggggta
     1321 tattggggtg catttgatta atccatggaa catataaaga atacttttag agatatagtg
     1381 aatttgatga taatacaata aatattttat actgatgaat tgtttttata ttatgctttt
     1441 tatttcaatc taggaaatat ataacttgtt taaacttatg attaaaagga taaagaagtt
     1501 taaagcatat aagtgctact taatctatta gagtaattac tagttataat gatatacaga
     1561 atcctataag attatgtata agtatagaac aaatttatta tctatgtggg atgctaattt
     1621 aaatttttta attgtattta aaaaaataag atgtaaaaaa ggaattgaaa aatgttaaga
     1681 taataaacac atagaattat taagtttaat actaacaaga ctattgtata tagacaatat
     1741 attatatata aaa
//
LOCUS       DAEPOZ010000380         1746 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.383, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000380 DAEPOZ010000000
VERSION     DAEPOZ010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.383"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(147..506)
                     /locus_tag="KR440_003648"
     CDS             complement(147..506)
                     /locus_tag="KR440_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH0700625.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(711..1490)
                     /locus_tag="KR440_003649"
     CDS             complement(711..1490)
                     /locus_tag="KR440_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBH0700626.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT          661 a          292 c          146 g          647 t
ORIGIN      
        1 gtttaaataa aacaatcaac cacaataata caaatacctt tttaggtaat tttttaaata
       61 aacatgctct tataaaaata actattaaaa gtaatactcc agaagtaaaa ctcatttcta
      121 acaatttaat gaacataaat cttacctcac tttaattttt caacaatttg tttgagtttt
      181 gtaatctcat cacttgaaag attttcttta ttaataaaag ctgaaagaaa tttttctgaa
      241 gaccctccaa acattttatt aattaaagtt tctgtttcct gtttttgtac ttcctctttg
      301 cttattaaag ctttacaaac aaaatttggc tcaattctct ctattgcacc ttttgacact
      361 aattttttta taacggtata cgtagtgttt ctattccatc ctgtttcttc ttttaaaatt
      421 tttactaact ctccagcctt gctttctcct ctctcccata gaacttccat tactttaagt
      481 tctgaatcaa ataattttat ctccatataa aatcgctcct caaatatagt tatcacaata
      541 gtcattacta taataaaaaa ctatcatagt agtctatact atgtcaatca tttatttttt
      601 aataataaaa actgtctttt taaaaaattc ttataaaaga cagttttatc aatgttatac
      661 tttaatgttt aattaaagcc atattttatt taaatgtata actatctatc ttagtacata
      721 gtataagatt tttctttatc taatagtata tcctctacat ctgggttttc ttttactaat
      781 tgtctataaa aattacaatt agtaacacct acatatggaa ttagccatag tagacctatt
      841 ccaagagtag ctattgaaag tatataccat cctataaaac taaagtttag tacaaataat
      901 ttaagtacat ttcctttcat catatctaaa ctagcaccca ttgatttaaa tacacccata
      961 ttatccttgt cttcacaaat taagaaagta gcttgagaaa catataatag caatatataa
     1021 accggtaaca taagaacaag tattataaga actagagtta ttccagtaga aataatagtc
     1081 cctattccaa cattatcact tgataaatat tcaattgctc cactagtaag taaaactcca
     1141 aatgataaaa acgctcctat aattgtgatt ataaaagaat aaatataaac aataattgat
     1201 tcaaataaat aaaaaccaaa acttcttaaa taaattctcc cacttacaaa gatatctcct
     1261 atcttgactt tttcatcttt agtaattttc aagaacatac atgtaaatcc tgctgaaacg
     1321 caaaaagcta ttacagccac tacaatagta gtcaatggtt caaaaggtaa tcctacaatt
     1381 gatattaata ctatcaatgc aatatatact gccatagaaa ttgcacatat tccccagttt
     1441 ccagataact gagctttaga tactcgtttt aattcttgcc ttgataccat aaaaaacacc
     1501 cctttcattt tataaaaaaa cttttaaaaa tataaataat cttttaacgt ataatattaa
     1561 tatatcatat tttcgactat ataagataca attattcacc atgttttatc aagatatgcc
     1621 ataattgtat tactagtttt atatagtcaa cattaccaaa tacatcaaat aaatttgatt
     1681 aattattcaa caagtaataa aataatttct attaattgta ctttatttag taaacaaatt
     1741 atttaa
//
LOCUS       DAEPOZ010000381         1693 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.384, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000381 DAEPOZ010000000
VERSION     DAEPOZ010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.384"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            277..1557
                     /locus_tag="KR440_003650"
     CDS             277..1557
                     /locus_tag="KR440_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBH0700627.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNIAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKVDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDM
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNSIYYKNNKDLVKGPMDFYTHPIYVK"
BASE COUNT          704 a          205 c          188 g          596 t
ORIGIN      
        1 catatttcta gtatggtaaa ttgtgtattt cactaataat atattaacaa gttaacttaa
       61 tgtagattat tctttcccat ttgttataat aatataatat aaagagctaa taaagttatt
      121 ttaataacat ttaaccagtt ttttatgtag gtaacatttc tacattttta taaatttttt
      181 tacgtttttt aattttataa ttaaaaattt aaattttata catattataa taaattgtga
      241 tatatataaa ataattaaag ataaaaggag tattttatga acatacttgg attaatagtt
      301 gaatataacc catttcataa tggacacata tatcatttat ctaaatctat agaaaaaaca
      361 aatgcaacac atactgtagc aataatgagt ggaaattttc tacaaagagg cgaaccagct
      421 ttatttgata aatatactag agcaaaaatt gctgtcgaaa atggtgtaga cttagttata
      481 gaattaccaa ctttatttgc ttgtcaaagt gctgaaattt tttctcatgg tgctataaca
      541 attttaaatt cactaaactg cataaattct atatgttttg gtagtgaaga aggaaataca
      601 gaaattcttt ataatatagc tgaaatttta gcaaatgaac caaatgaatt taagttactt
      661 ttaaaaaaat atttagatga tggcctgtta tttccaacag ctagagctaa tgcacttttt
      721 gattatatac atcaagacaa aaaaaataat tttatacaaa attattcaaa ggaacaatta
      781 tcaaaaacat taaattcatc aaacaacata ctgggtatag aatacataaa aaacctcatc
      841 catttaaaca gtagtataaa accatttact atctccaggg tccaatcaga atataattcc
      901 gaagaaattg agagtgatat ttgttctgct actgctataa ggacttcact taaagaaaat
      961 acagaccttt ttcatttaaa aaatgttatt ccagaaaaaa cttataacat cctaaatgaa
     1021 aaagtagata atggtttttt acctatgttt gatgacttct tctttgatat tttaaaaaca
     1081 attatactta gagatatgga cactttaaaa gagtattttg aagtaaatga aggcatagaa
     1141 aacagaatct ataaagatat ctttacatct gaatttctac atgagctaca attagcaatt
     1201 aaatccaagc gatacacttt aaccaaaatc aaacgaactc taaacaatat attgttaggt
     1261 attaaaaaaa gcgacatgca acttataaaa aacatagata ctatacctta tataagaata
     1321 cttgctttta atgataaagg tagagaaatt ctaaaagaga taaaaaataa atctgaaata
     1381 aaaataataa acaagttttc aaatatatca ttttcattag atgatacgat ttttaaaaca
     1441 ctcataagtt atgatataaa agctacaaat atatataatt caatatatta taaaaacaat
     1501 aaagatttag taaaaggccc tatggatttt tatactcatc caatatatgt aaagtagttt
     1561 tttaaaataa ctatatttat aaaaatcatt ttattaactc tttattatat aagatacaat
     1621 agtcttactt tatttatgta cacatgcttt taggatgaca acatcaactt aaatattatg
     1681 ttactttcta aat
//
LOCUS       DAEPOZ010000382         1673 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.385, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000382 DAEPOZ010000000
VERSION     DAEPOZ010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.385"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..915)
                     /locus_tag="KR440_003651"
     CDS             complement(<1..915)
                     /locus_tag="KR440_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700628.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLIIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKELMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMG"
     gene            1040..>1673
                     /locus_tag="KR440_003652"
     CDS             1040..>1673
                     /locus_tag="KR440_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBH0700629.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQIS"
BASE COUNT          615 a          256 c          183 g          619 t
ORIGIN      
        1 tcccattaag taatatatag ctttcatatc attgtttatt atatatacat tttcttttac
       61 atggtcatta ttaaaattaa atctagtaaa aaatatacct tcattcatat tactttttaa
      121 cagaaatgga ttttctctat aaaatactcc tgcaaattct tcaggtgaat ttacactgta
      181 aacacttaca aattcacttt tagaaatctc atcaaatacc tcaaaatcat acataagttc
      241 tttatctgtc actaatagag aattaattct aaaaccagtt tcatagcact tacttatgca
      301 aaaagcaatt ttacatatat aatatccatc attgtcttca tataaagctt cacttatata
      361 tctgccttga cctttatcac ttaccacgtt tttaagtaat gttccattta tattagtaat
      421 atgcatattt gcaatctcgt cacttccaga aaaatatttt aaactttctc tatcccatgc
      481 tataaatctc atagtcatat agtttataaa ttcaacttcc tcatcaactc ttttacatat
      541 tttattgtat aatttttcta taattaaatc tgttttatag ttttttataa tatcaatata
      601 ctcacattta tctcctggca atagcttatc ataataaaga ttcttattac caaaataaga
      661 tactaaaaat aaagattcat ccttcgttaa ttcaactcta tctgaaccaa atcctcccat
      721 caatctttct tcttccatat aagcttcttc ttgtgttggg ttatttaaac tcacataatc
      781 tgctaatcct aatccctctg catctaataa aaaatattga caaaagtgat tgttttcatc
      841 atctatccaa tttattataa gacctaaact gcccataagt ctagaatttg tagcctttgc
      901 aaaattaaaa ttcatatagt cacatccttt aaactctaaa atacttgttt tttaatatat
      961 aaattattat acatatattt taacaactta gatacaattt ataatataat ataaggaaat
     1021 gattaattga ggtggtctta tgaaaaatat agcagctttt tttgatatag atggtactct
     1081 ttatagagac tctcttatgg ttgaacattt taaaaaatta attaaatacg atataattga
     1141 ccaaaaaaat tggtataaac atgctagaga cacttttatg gattgggata aaagacaagg
     1201 taattatgat gattatctat tagaaatatg tgacctttat gttgattcac taaaaggatt
     1261 agataaaacc tgtatcaact ttacaagtga ccaagtaatt aaattaaaag cagatagagt
     1321 ttataaatac acacgttcaa ggattgagtg gcatctaaat caaggacaca tagttatatt
     1381 tatatctggt agccctggat ttcttgttga aaaaatggca aaaaaatata atgtaaccga
     1441 ttttctagga agtgattatg tctttgaaaa taatatattt acaggcacag ttattccaat
     1501 gtgggattct ataagtaaga ataatgctat aaatgatttt gttgtaaagt atgatttaga
     1561 tttatcaaac tcgtatgcct atggagatac taatggtgat ataaatatgc taaaaagagt
     1621 tggaaatcca atagcaataa atccaacaaa ggaactttta tctcaaatat cac
//
LOCUS       DAEPOZ010000383         1665 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.386, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000383 DAEPOZ010000000
VERSION     DAEPOZ010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.386"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            107..1543
                     /gene="miaB"
                     /locus_tag="KR440_003653"
     CDS             107..1543
                     /gene="miaB"
                     /locus_tag="KR440_003653"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBH0700630.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
BASE COUNT          704 a          170 c          305 g          486 t
ORIGIN      
        1 actattaaaa cacaactaga aaaataataa tatctttaaa agatttttct tatttgatat
       61 aattaaggtt atgtgtaata taagaaatta actaggagga atagacgtga gcaaaagaaa
      121 acaagtaaca atacctatag agaaaataca agaacaagat aaatatatag aacaaataca
      181 cagacaaaat gaagaatact tcaacagaac tggaaagaga aaactcgtgt ttactcaaac
      241 ctttgggtgt caaatgaatg aacatgattc agaaaaatta tgttcgatgt tagaagagat
      301 gggataccaa atgtcaatga tggttgaaga aagtgattta ataatatata acacctgtgc
      361 agttagagaa aatgcagagc taaaagtgta tggtaactta ggacaattaa aacatctaaa
      421 aggaaaaaat ccagatatga aaatagctgt atgtggatgt atgatgcaac aaccacatgt
      481 tgtagaggaa ttaagaaaaa aatataaaca tgtagacctt atatttggaa cacataatct
      541 atataaattt ccgcaattac ttacagaatc aataaattct gacaaaatgt tagtggatgt
      601 atgggatgtg gatggagaag taatagaagg tcttagaagt aatagaaaat ttgaattaaa
      661 agcatttgta aatataatgt atggatgcaa taatttctgc acatattgta tagtcccata
      721 cacaagagga agagaaagaa gtagaactcc tgaagatata attaacgaga taaaagagct
      781 tgttgcaaat ggaacgaaag aaatcacttt acttggacaa aatgtagatt cttatggtaa
      841 gacacttgaa aatccagttt cattctcaga acttttaaga aaagtgaatg atatagaagg
      901 aattgaaagg gttcgattta tgacatctca tccaaaagat atatctgatg aagtaatata
      961 cgcaattaga gattgtgata aggtgtgtga gtttttacat ctaccaatac aatgtggaag
     1021 cagtagcctt ttgaaaaaaa tgaatagaca ttatacaaag gaatattatt tagaaataat
     1081 agaaaaagct aaaaaggaag ttccaggtat tgctttttct acagacttaa tgattggttt
     1141 cccaggagaa acagaagaag atttattgga cactttagat gtggttgaaa aagttagata
     1201 tgattcagct tttacattta tttattctaa aagacaggga actcctgctg ctaaaatgga
     1261 aaatcaaata ccagaagata taaagcatga tagatttaat agggtgttag aagctgtaaa
     1321 taggatttct gcagaaataa atgatggata taaagataga atagttgaag ttctagttga
     1381 aggaagaagt aaaaataatg aaaataagtt tgctggtaga actagacaaa ataaactcgt
     1441 aaatttcgaa ggtggaaatg atgatttaat tggtaaatta gttatggtca aaattactga
     1501 acctagaact ttttcattaa atgggatatt agtaaataat taatattgta caaaataata
     1561 aaattatgta aactaatcga aatagatgta ctatagaaca aattatatta aaaattttta
     1621 ttttaagcga atttgtcata tgttatgcta atgtaagttt ttgct
//
LOCUS       DAEPOZ010000384         1662 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.387, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000384 DAEPOZ010000000
VERSION     DAEPOZ010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.387"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..>1662
                     /locus_tag="KR440_003654"
     rRNA            <1..>1662
                     /locus_tag="KR440_003654"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          477 a          347 c          492 g          346 t
ORIGIN      
        1 gggacacgtg aaaccctgtg ggaagcagga gggaccaccc tccaaggcta aatactacct
       61 agtgaccgat agcgtatagt accgtgaggg aaaggtgaaa agaaccccgg gaggggagtg
      121 aaatagaacc tgaaaccctg cacttacaag ctgtggaagc acatttcttg tgtgaccgcg
      181 tactttttgt agaacgggcc aacgagttac gttaagtagc aaggttaagc acttaaggtg
      241 cggagccgta gcgaaagcga gttttaactg agcgttcagt tacttgacgt agacccgaaa
      301 ccgggcgacc tacccatgag caggatgaag cgaaagtaaa atttcgtgga ggtccgaacc
      361 cacgagcgtt gaaaagctcg gggatgactt gtgggtagcg gtgaaattcc aatcgagccc
      421 ggagatagct ggttctcccc gaaatagctt tagggctagc ctcaaggtga gagatacgga
      481 ggtagagcac tgaatgtcct agggggtatt gcacctaccg aagactatca aactccgaat
      541 gccgtcatct tatacttggg agtcagactg tgggtgataa gattcatagt cgaaagggca
      601 acagcccaga tcgtcagcta aggtccctaa atgtaagtta agtggtaaag gatgtgggat
      661 tgcacagaca accaggatgt tggcttagaa gcagccactc attcaaagag tgcgtaatag
      721 ctcactggtc gagtgatcct gcgccgaaga tttccggggc taaaacttac taccgaagct
      781 acggcatcag taatgatggg taggggagct tcccatacgg gttgaagcat gaccgtaagg
      841 acatgtggac agtatgggag tgagaatgtt ggcatgagta gcgagatgtg ggtgagaatc
      901 ccacaggccg taaacccaag gtttccaggg gaaggttcgt ccgccctggg ttagtcggga
      961 cctaagctga ggccgaaagg cgtaggtgat ggacaacagg ttgatattcc tgtactaccg
     1021 ataaccgttt gagagaaggg atgacacagt aggataagct aagcacactg ttggttatgt
     1081 gtgcccaagc attgaggcag tcaaagtagg caaatccgct ttgataatgc tgggatgtga
     1141 tggggagcga aatttagtag cgaagtagct gatttcacac tgtcaagaaa agtctctatc
     1201 gaggttaaag gtacccgtac cgcaaaccga cacaggtggg tgaggagagt atcctaaggc
     1261 cagcgagaga actgttgtta aggaactcgg caaaatgacc ccgtaactta gggataaggg
     1321 gtgccaccat caggtggccg cagagaatag gcccaagcga ctgtttacca aaaacatagg
     1381 tttctgctaa gtcgcaagac gatgtatagg agctgacgcc tgcccggtgc tggaaggtta
     1441 aggggatctg ttagagcaat cgaagcagtg aacttaagcc ccagtaaacg gcggccgtaa
     1501 ctataacggt cctaaggtag cgaaattcct tgtcgggtaa gttccgaccc gcacgaaagg
     1561 cgtaacgatt tgggcactgt ctcaacaaca gactcggtga aattgtaatt ccggtgaaga
     1621 tgccggatac ctgcgacagg acggaaagac cccatggagc tt
//
LOCUS       DAEPOZ010000385         1651 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.388, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000385 DAEPOZ010000000
VERSION     DAEPOZ010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.388"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..>1651
                     /locus_tag="KR440_003655"
     CDS             <1..>1651
                     /locus_tag="KR440_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700631.1"
                     /translation="EYGNEALDKYRELLDKTDNNSRCILLLVPNNNTKIRYERALRLN
                     YSEELKITTYISFVKKELVKYWPLIIEKCDEINTKVVSPTFISNSLSEYLIVQKVKEK
                     RIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKKNKDSIYRFSYT
                     QMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVRYIIVDSLESSS
                     NAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIKPKEENIKKKEI
                     RIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEENVSPRDIAIISPINNSILEH
                     QIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKDDEYLSFLETLL
                     EINRIKAFKIFNETRHLSIDNIDEHSQYRDILQYIEEKKKSDIKIHEFLTQFYIDKML
                     NLKEGKKNVGLCKKIIAESESFSESLSLLRMKKEKIFIEALKSTINDYYSVVDIEELK
                     SKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDIS"
BASE COUNT          735 a          135 c          251 g          530 t
ORIGIN      
        1 agaatatgga aatgaagctt tagataaata tagagaatta ttagataaaa ctgataataa
       61 ttcaaggtgt attcttttat tagtaccaaa caataacaca aagatacgat atgaaagggc
      121 tctaagatta aattatagtg aagaactgaa aataacaaca tatataagtt ttgtgaagaa
      181 agaattagtt aaatactggc cactaataat agaaaaatgt gatgaaataa atacaaaggt
      241 tgtatctcca acatttatat caaatagttt aagtgaatat ttaatagtgc aaaaagttaa
      301 ggaaaaaagg atacaagaag gatattttga agatattaca ggaaccaata aatccatagc
      361 aaatagtata atgactaaca taaataaatc tgcatttaat ttaattgatt ttgattttat
      421 tggagaaaaa atatattcat caaagaaaaa taaagatagt atatatagat tttcatacac
      481 tcagatggat gaaattatat catattatat aaattcgtta ctatcaaatt caattttaga
      541 taatgcatta agcatatatt tatacaatca atacctactt aatgatgatt tttatttaaa
      601 aaacttattt acagaagtga gatatataat tgtggatagt cttgaaagct caagtaatgc
      661 agaagttgat tttataacag aagcattgga taatacatta caaagttata tatattttga
      721 ttatagtagg gattattctg tatttaataa tgtggatata gactatataa atgagaaaat
      781 aatatcaaaa attaagccta aagaagaaaa tattaaaaag aaggagatta gaatagaaga
      841 tttatactta ttacctgcaa atatagaatt aaatcaatca agtcaattat acaatgaaat
      901 gttagattta ataagtgaaa aagttatatc tcttatagaa gaaaatgtat ctccaagaga
      961 tatagcaatt atatctccta taaacaatag tatattggag catcaaatta gagattcttt
     1021 gatagaaaaa aatatagatg tttttagtac taagaaggat aaaaaagctg tagattatcc
     1081 atatgggaat gcattggtag tggctacatg tatattttat ggatatttag attttataaa
     1141 agatgatgaa tacttaagct ttttagaaac tcttttagaa ataaatagga taaaagcatt
     1201 taagattttt aatgaaacaa gacatttaag tattgataat atagatgaac atagtcagta
     1261 tcgtgatatt ttacagtata ttgaagaaaa aaagaagagt gatataaaaa tacatgaatt
     1321 tttaactcaa ttttacatag ataaaatgtt aaatttaaaa gaaggtaaaa agaatgttgg
     1381 gttatgtaaa aagataatag ctgaaagtga gtcttttagt gaaagtttaa gtctacttag
     1441 gatgaaaaaa gaaaagatat ttatagaagc tttaaaaagt acaataaacg attattactc
     1501 tgttgtcgat atagaagagt tgaaatccaa agacaaaata gtaataacta ctccttattc
     1561 atatatatca tcaaatatag atagaagtat acaaatatgg gtagatatag gaagtaatgc
     1621 ttggaatatg aaaatagaaa aagatataag t
//
LOCUS       DAEPOZ010000386         1649 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.389, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000386 DAEPOZ010000000
VERSION     DAEPOZ010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.389"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            104..1540
                     /locus_tag="KR440_003656"
     CDS             104..1540
                     /locus_tag="KR440_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HBH0700632.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSARLKNVSKV
                     YIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVNTVMLTNAY
                     KGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTLVNNTKSTR
                     LGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPIVLVNDGSNKSI
                     LKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGMYKVGKDIS
                     AGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYIKIEDSTMT
                     LAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLGNAEGIVTN
                     DTFSGRRYITVEEGQYLILNDCYLIENE"
BASE COUNT          674 a          166 c          272 g          537 t
ORIGIN      
        1 cttaatataa tttgcaattt tacataagtt tgttaagtac ataactaatt taagaatatg
       61 tatttatata ttaaaaattt attatgaggg ggacaatttt attatgaaat tctataaaag
      121 aatattaaca ttgactttaa gtatttcttt tgtatttgca aatattcaat tagtagatgc
      181 aatatcatct gtagaaaaaa tacaaggaaa aaataagtat gaaatagcag gcaaaatagc
      241 agataaaaat gcatataaaa ctgctatatt aattaatact agtaactcta ttgcagatgg
      301 attaagtgca agtggactag caggagcttt aaatgcccct atactattaa ctgaaaaaaa
      361 tactatacca actgagacat ctgcaagatt aaaaaatgta tcaaaagttt atataattgg
      421 aggaacatat tctattagta catctgtcga aaattcatta aaatctaaga aaatgaaagt
      481 tgtaagaatt aagggaaatg acagaataaa aacaagttat aatgttgcaa aagaaataaa
      541 ttcaataaaa aaagtcaata ctgttatgct aaccaatgca tataaaggag aagcagatgc
      601 tataagtatt gcatctgtag cagcaagaga taaagcacca ataattctta ctaatggaca
      661 aagtatacct ttttcaacta gtggtttaaa gtcttatgta ataggaggaa cagcttcaat
      721 gagtaccact ttagtaaaca atactaaatc tactagacta ggagggagta ctcgttttga
      781 aactaataaa gcaataataa ataagtttta taaggatgct agagagtttt atatagctgg
      841 agcttatgaa ttaacaaatg cacttgtagg atcttcttta tcaaaacatg gtccaatagt
      901 tttagttaat gatggtagca ataaaagcat tttaaaaaat gctaagaaga taacttctat
      961 aggatatata gattcaaata tagtacaaca gtgtttaaat ataacaaatg gaattggaga
     1021 tattaatact ggggttgtaa aaaatgttaa acctactaca aagactataa aagatggaat
     1081 gtataaggtt ggaaaagata tatcagcagg tgaatactta attacttcaa actcaggaag
     1141 ttatgcttct tattatgaag ttacaagtga tagtacaggt aatgctgata gtatactttc
     1201 aaatgatatt tttagtggga ctagatatat caccttaaag aatggtcaat atattaaaat
     1261 tgaggattca acaatgacat tagcaaagta tgctaaagcc cagaaggcca agaatggtaa
     1321 gtttggaaat ggtatgtata aaattggatt agaaattcct gctggagaat atattataat
     1381 gtcaaattct tctgatgcat attatgaagt tagaaatgat agcttaggta atgcagaggg
     1441 aatagttact aatgatactt tttcaggaag aagatatata acggttgagg aaggtcaata
     1501 tttaatatta aatgattgtt atttaattga aaatgaatag atgtaattgt atttaatagt
     1561 aaatcatata ttttatttaa ggaaagatgt tttaagtttt aatttaaaac atctttttat
     1621 tattataata attatatgat tttatattt
//
LOCUS       DAEPOZ010000387         1634 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.390, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000387 DAEPOZ010000000
VERSION     DAEPOZ010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.390"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(314..1021)
                     /locus_tag="KR440_003657"
     CDS             complement(314..1021)
                     /locus_tag="KR440_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBH0700633.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
BASE COUNT          519 a          282 c          141 g          692 t
ORIGIN      
        1 aatacctttt ttgctttcat tgttaaaact attttttcta ttagtttatt tcttataaaa
       61 caaacattta gtattaaaat atatatcaca caatagtatt tgcaatttaa ctttaaaaaa
      121 ataaaaatac ccaactgacc taagcaactc gattgggtat aaataatcta aaaaatcatt
      181 tttgtataaa ctctttattt aacttacatt tttatttacc ttctacttta attcaactta
      241 tatttctatg cttcttatac tctcattcaa cttatatttc tacttatctt atacttttat
      301 aaattcatca caattattta gttctcgcta tcaaagaaat tgtattaaaa tactttccat
      361 tctctacttc catcattttt ttgtcattta tagcatatga attatcacaa tttatccaat
      421 ccttccaagc ttcatcatga caatttaaag cttcacattt ctctacaatc acagactccg
      481 attcagacca aagtttattc caccatgtaa ttgaatggaa gttcatatta tcttgccaaa
      541 atggaatgag ttcatctggt atacaatcca caaaatcttc ttttaatcca ggcattgcca
      601 tagcaagtat tcccccctct tttacaagag gtgatatatg attgtctaaa aatccttctt
      661 tgtttccaaa atagtgataa gaatcgatac ttatcaccgc atcaaagaaa ccttctgcat
      721 aaggtagttc atgtgcctct gcctgtattg gaaatatttt atcatccaat ttaaactctt
      781 taaatctttt atagttttca gttggttcta tccataaatc tgttgcaaat acagtagcat
      841 caaattcctt tgctaaaaat atcgaagata tacccttacc acaacctaag tctaaaatac
      901 gcattccctt ttctagtttt atcttacttg ttaattcttc caatattttt atacaattag
      961 gtcccatcat atttttcatg agaaagtcct tatcgtattt attgcttttt gtaaatatca
     1021 ttctatcttt cctttcccta tcagttatta tttattaaat tttatatgtt tgatttttta
     1081 tatgcttctt atattgagca atctcttttt tattcattct ttaacagaca attttaatgt
     1141 gtaattttat ccactctgtg atagtaactt taatgaataa tcttatctaa tatgtgataa
     1201 ttaactttga tgggtaattt tatctactct acaataatca ccttaatgag tatcttatct
     1261 actctattat agttatttta gtgagtaatt ttatctactc cacagtaatc gctttaatga
     1321 ataatcttat ccactctatt atagttattt tagtgagtaa ttttatctac tctatagtaa
     1381 ttgctttaat gagtaatctt atccactcta ttatagctat ttttatgggc aattttatct
     1441 actctataat agtcacttta atgagtaatc ataattttta tatcatttta gatacttact
     1501 ctaataagaa gtcattttct aatacttaat aagttttaat tacttccact ttaacctata
     1561 tactaattaa cttatttaat ttagtgcata tttgacactt taccttttat tataccattt
     1621 attatcataa atat
//
LOCUS       DAEPOZ010000388         1624 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.391, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000388 DAEPOZ010000000
VERSION     DAEPOZ010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.391"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            78..194
                     /gene="rrf"
                     /locus_tag="KR440_003658"
     rRNA            78..194
                     /gene="rrf"
                     /locus_tag="KR440_003658"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            202..276
                     /locus_tag="KR440_003659"
     tRNA            202..276
                     /locus_tag="KR440_003659"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:234..236,aa:Asn,seq:gtt)
     gene            282..367
                     /locus_tag="KR440_003660"
     tRNA            282..367
                     /locus_tag="KR440_003660"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:316..318,aa:Leu,seq:taa)
     gene            383..458
                     /locus_tag="KR440_003661"
     tRNA            383..458
                     /locus_tag="KR440_003661"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:416..418,aa:Met,seq:cat)
     gene            466..540
                     /locus_tag="KR440_003662"
     tRNA            466..540
                     /locus_tag="KR440_003662"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:499..501,aa:Glu,seq:ttc)
     gene            555..628
                     /locus_tag="KR440_003663"
     tRNA            555..628
                     /locus_tag="KR440_003663"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:587..589,aa:Gly,seq:tcc)
     gene            634..709
                     /locus_tag="KR440_003664"
     tRNA            634..709
                     /locus_tag="KR440_003664"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:667..669,aa:Val,seq:tac)
     gene            715..791
                     /locus_tag="KR440_003665"
     tRNA            715..791
                     /locus_tag="KR440_003665"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:749..751,aa:Asp,seq:gtc)
     gene            802..876
                     /locus_tag="KR440_003666"
     tRNA            802..876
                     /locus_tag="KR440_003666"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:834..836,aa:Gly,seq:gcc)
     gene            886..959
                     /locus_tag="KR440_003667"
     tRNA            886..959
                     /locus_tag="KR440_003667"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:920..922,aa:Arg,seq:tct)
BASE COUNT          514 a          278 c          342 g          490 t
ORIGIN      
        1 taactctaaa gaaactagta ttgctaggtt ctataaactt attaaaaact taatagaaat
       61 attaagcata aagattatgt ggttacaata gcagagagga tacacctgtt cccattccga
      121 acacagaagt taagctctct agcgctgatg gtacttggtg ggaaactgcc tgggagagta
      181 ggacgtagcc acgttaaatg ttccaaggta gctcaacggt ggagcaaccg gctgttaacc
      241 ggtaggctgt gggttcgagc cccacccttg gagccaaaaa agccgaagtg gcggaactgg
      301 cagacgcaca ggacttaaaa tcctgcggga cttacctctc gtaccggttc gattccggtc
      361 ttcggcactt ataaaacaat atcgcggggt ggagcagttg gcagctcgtc gggctcataa
      421 cccgaaggtc gtaggttcaa gtcctgcctc cgcaaccatt aaaacggctc gttggtcaag
      481 aggttaagac accgcccttt cacggcggta acaggggttc aattccccta cgagtcacca
      541 attcaattaa atatgcgggt gtagctcaat ggtagagttc tggccttcca agccagctgt
      601 gagggttcga tccccttcac ccgctccaaa aatgggacca tagctcagct gggagagcac
      661 ctgccttaca agcagggggt cacaggttcg agccctgttg gtcccaccat tcgcggcctg
      721 gtagttcagt tggttagaat gccagcctgt cacgctggag gtcgagggtt cgagcccctt
      781 ccaggtcgcc aaattaaata agcgggaata gttcagtggt agagcgcaac cttgccaagg
      841 ttgaagtcgc gagttcgaat ctcgtttccc gctccataat aacttgggtg catagctcag
      901 ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct gtgcgctcat
      961 taagaaaagt ttactataaa ttatagtaaa cttttatatt gtttaaaaag acacaggaaa
     1021 catgtatatt acctgtgtct ttttatttat aaactttaaa ctatgtaaaa attacagtat
     1081 attctaatag agttacagta tattcattaa tattgtaaac atacggaggg taatgtataa
     1141 tagtctacaa gatgtttatg aaggcatcag ttaaatataa ctaattagat aagagttata
     1201 tacattatat tgtatacttt atggtattta tatattgaaa taacttatga aattatttat
     1261 aaagagattt atgagcatat ttttgtctaa acaataagta taagaaatta taaaaaatta
     1321 gagactatag tgaattgaca atttaatgta atatttataa gttatactaa gtaaattatt
     1381 atactgtgaa atggatatat aactttaggt attgtgtatt attttcactg taggagatta
     1441 tatctagtta tacataataa gttttaatga tagtttatag taagatatta aaataaattt
     1501 atctaatcta gatatgaaat aagttattac tttaatataa aaatgaatct aatggaataa
     1561 tattgatgat aagtaatatt tagattttgt atttgtttat agttaataag tggattatag
     1621 aatt
//
LOCUS       DAEPOZ010000389         1623 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.392, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000389 DAEPOZ010000000
VERSION     DAEPOZ010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.392"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..573
                     /locus_tag="KR440_003668"
     CDS             <1..573
                     /locus_tag="KR440_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBH0700634.1"
                     /translation="KNEIQKEIKHEQQRINKIDKFIEAINCDKDEIHYNVVFKKIPSY
                     KIISLREVVPDYQSEGILWEKLSKFIKEEHIEVSRQPNNNIAFYHDEEVKDNGVDIEV
                     GMVVKKIGKNKSGFIYRETEEIDMMACTMVYGPYENIAGAYESFCYWLDKNSDYQISG
                     INRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            672..1280
                     /locus_tag="KR440_003669"
     CDS             672..1280
                     /locus_tag="KR440_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBH0700635.1"
                     /translation="MEKLLRGKIMKADSTKEQLINATIQLLSSHRDVSSITAREIVAK
                     ANVNLAMINYYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSF
                     IQLLFLRSNAFFKYSGIDILNVEQRNGLIDFQLDLLLVDITK"
     gene            1384..1560
                     /locus_tag="KR440_003670"
     CDS             1384..1560
                     /locus_tag="KR440_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700636.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
BASE COUNT          615 a          188 c          247 g          573 t
ORIGIN      
        1 aagaatgaaa tacaaaagga aataaagcac gaacaacaaa gaataaataa aattgataaa
       61 tttatagaag ctataaactg tgataaagat gaaattcatt acaatgttgt ctttaaaaag
      121 attccaagtt ataaaatcat atcacttaga gaggtagtcc cagactacca atctgaaggt
      181 atactctggg aaaagttatc taaatttatt aaagaagaac atatagaagt atcacgtcaa
      241 cccaacaata atatagcttt ttaccatgat gaagaagtca aagataatgg tgtagatatt
      301 gaagttggaa tggttgtaaa gaagattgga aagaacaaat ctggatttat atatcgtgag
      361 acagaagaaa ttgatatgat ggcatgtact atggtttatg gtccatatga aaatatagct
      421 ggtgcatatg aatcgttctg ttattggtta gataaaaata gtgattatca aatatcaggt
      481 ataaatagac aaatcggtca taagggtgag cataatgaaa taaatcctga aaattatctt
      541 acagaaattc aaatccccct tataaaagtc taatcgatta gtacttaaat acttaagtac
      601 taatcaccca ttgacatccc aaaattttca tgatagtatc taaacgtgta ttaaacatgt
      661 ttaatttttt tatggagaaa cttttaaggg gaaaaattat gaaagcagat tcaacaaagg
      721 aacaattaat taatgccaca atccagttac tatcttcaca tagagatgtt tcaagcatta
      781 cggctagaga aattgtagct aaagcaaatg tcaatttagc tatgattaat tattatttta
      841 aatctaaaga ggagttaatt aatatttcta ttaaaaaatt tttaaagcgt catatggatg
      901 aatataaggc tacacatgat actaaaaatt tagcacctaa acagcaatta agggaaatac
      961 taattgcatt ttttgatttt ataggtgaac atagccaatt tataaagatt tctgtaccct
     1021 atatattgat acaagaagaa attgtttatc catttgagat actaccttta attaaggctc
     1081 actatgaaaa tcagaataac aaaaaaactg aagctgaata cagaactatt gcgtatcaaa
     1141 taacttcatt tattcagttg ctttttcttc gttctaatgc ttttttcaag tatagtggta
     1201 ttgatattct taatgttgaa cagagaaatg gtttaataga ttttcaatta gatttattat
     1261 tggtagatat tacaaaataa tactatatta attttttatg tacatttttt tattttatgg
     1321 taacgtttga aaatattctc attgactaaa aatacttgca ttaaataagg gggaggaatt
     1381 ttaatgtttt ttacatttat aggagtaata tcagcaattt tattgttgtg tacaattata
     1441 tgtggtttat ggataaaact taataatcgt aataatgact ctaattctca ctttactttt
     1501 tcaatacttt caattgcttt ttcactgtta attgtggtgg cctttttagt aagaaattaa
     1561 tgtctttgtt atgctggggt ttattattat agatttttat tattgcatga tttttactat
     1621 att
//
LOCUS       DAEPOZ010000390         1622 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.393, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000390 DAEPOZ010000000
VERSION     DAEPOZ010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.393"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            307..966
                     /gene="cprR"
                     /locus_tag="KR440_003671"
     CDS             307..966
                     /gene="cprR"
                     /locus_tag="KR440_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HBH0700637.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
     gene            1177..>1622
                     /locus_tag="KR440_003672"
     CDS             1177..>1622
                     /locus_tag="KR440_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBH0700638.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFA"
BASE COUNT          638 a          121 c          272 g          591 t
ORIGIN      
        1 ttttcctcca atttaaacaa ttaaaagtgt atttataaaa agttttctta ttattaattt
       61 tattatatat tcaaaatatt acaataataa attgttaaat aaatttttat atttaagtaa
      121 atgcgtgtaa aatgaatata aagtttttga aatataaagt ttttgtatct aaaatatttt
      181 atttgtaaaa tagtaattaa ttattgtaag ctataatatg agagtattat ggttaatgtg
      241 aattttaaag atattattaa agatataaat tagaatacta ttaaacggat aaatggagga
      301 gttattatgt ctaagatatt agtagtagat gatgagttag atatgttaaa gttgattgaa
      361 aatgtgttga aaagagatgg acatgaagta aaggtaatta gtgatgtaga aaaggctatg
      421 aacatggatt ttaattattt taatctaata atattagatg ttatgatgcc taagatggat
      481 ggttttgaag tatgtaaaac tataagaaat aaggttgatt gtccaatact gtttttgaca
      541 gcaaaaaata tggaaagtga tattatgtac ggtcttggca taggtgcaga tgactatata
      601 accaaacctt ttggtgtagg agaattaagg gctaggataa ctgctcattt aaggcgagaa
      661 agtagagaaa aaaagaattt attaactatc tcaaatgttc aatttaactt tttaggcaaa
      721 gaagtttcaa ttgatggtaa aaaaattaat tttactaaaa gtgaatatct aatatgtgaa
      781 tttctagcaa aaaataaggg acaagtattt tcaaaagaaa gaatatatga gtcggtatat
      841 ggttttgatg gagaaagtga tataagtact atcacagagc atataaaaaa tatacgaagc
      901 aaattaaaac aatatgatat ttatacaata gaaactgtat gggggatagg gtataaatgg
      961 gtgtaataag tataagtgtt gcttagtgaa aatattatat atatctacaa aatgtttgta
     1021 aaatattaat ctatatatta aaatttaatt tatagattaa tattttttgt aaatgacaaa
     1081 attgtaaggt attttgttat aaaatattaa gattcatagt gtatactaat aatatagtat
     1141 ttagtataaa tcttgagcaa aggatggtta tttctaatgg atagactaaa gaatattaga
     1201 aagagtttac tacagtatac atttttattt ttgttaatag gtataacaat ttacctagtg
     1261 tttaaaacat tggatattaa aatgctatca aatgttatgg ttatggtaga taagaagttt
     1321 ttattcatag gtgctttggc aataatgtgt cacataatgc ttgaaggagt agtaatgaag
     1381 ataattatag aaagcactca caaagttaat attagattta ttggttttaa gctagccatt
     1441 atgggatttt attataattt aataactcct tttgcttcag gaagccaacc agtacagata
     1501 tatgtactta aaaaatgtaa aatgccactt agtaaggcaa gtgcagttgt taccaataag
     1561 tctattattt tccaaatagt ggttactttt tattgtacaa tacttgtatt aatgaatttt
     1621 gc
//
LOCUS       DAEPOZ010000391         1574 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.394, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000391 DAEPOZ010000000
VERSION     DAEPOZ010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.394"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..582
                     /locus_tag="KR440_003673"
     CDS             <1..582
                     /locus_tag="KR440_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HBH0700639.1"
                     /translation="VRKKLKELDPHTISNLSNIPFNNDLNRFTLNFFDRQYIIDYPSG
                     EVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIPGGNVYYPNFLNRTLYR
                     LSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMIFILWFGDDEFPPNSNI
                     LFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
     gene            650..1483
                     /locus_tag="KR440_003674"
     CDS             650..1483
                     /locus_tag="KR440_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HBH0700640.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIRMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPLGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNRA
                     KKDKRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
BASE COUNT          594 a          208 c          236 g          536 t
ORIGIN      
        1 gttcgaaaaa aactcaaaga attagaccct catacaatta gtaatttaag taatatacct
       61 ttcaataatg atttaaatag attcacactc aatttttttg atagacaata tattatagac
      121 tatccaagtg gagaagtttg gaatagtaat ggtagccttt gcaataactt tgagcttaaa
      181 atcttgatag ttagatattt aatcaatgct aaaggtatcc ctagaactaa taaatatgta
      241 acttttaaag aaataccagg aggcaatgtt tattatccaa attttttaaa caggactcta
      301 tataggcttt cagaagtttt tacatctcaa atagacaaat ataagttagt tatggagaaa
      361 attggagctg aaaaagtaaa catgggagat ttagcttata ggtttacata tatgggtaat
      421 acatctatga tatttatact ttggtttgga gatgatgaat ttcctccaaa ttcaaatata
      481 ttatttgatt ctaatatagt ttattacttc aatgcagaag atttagcagt tgttcctgat
      541 acagctataa atgcaatttt aaataaaata aatcaattat aaatataaat aatattataa
      601 taaagttttt gatagattta atagaatata atatgaaatg gggtaaaaaa tgaatgtaga
      661 aaatcaatct ttatcagata ttgaaataat attatcacac aggtatgata atggtgcaga
      721 ctattggact acacctgata agcgtttaat aaaaggttct cctttctcta ctcttgatag
      781 tgtactttat ctattagagt taggtgtgaa ccctgatgat actttactta aagaatgtgc
      841 tgacttgata tttagcacat ggcgagaaga tggacgtttc aaactatatc caaatggagg
      901 tatttaccct tgtcatactg caaatgctgc taacgtactc tgtcatatgg gatatgcatc
      961 tgacattaga atgcaaaaaa cgtttcaaca ttttttagat acacagtata ctgatggtgg
     1021 gtggcgttgc aataaattta gttttggtcg aggtccagaa actgaatact caaatccact
     1081 accaactctt aatatattaa atgcattccg ttttagtaat tatctaaata aagaatcaaa
     1141 acttgacaaa gctgtagatt ttctacttga ccattggact attaaaaaac cactcggccc
     1201 ttgtcactat ggaattggca cattatttat gcaagttgaa tatcctttcc gtaattataa
     1261 cctattcctt tatgtctacg ttttatcatt ttataatcgt gcaaaaaagg ataagagatt
     1321 tttagaagca ttgaagatac ttgaatctaa aatgataggt aataaaattg tcgttgaacg
     1381 tgtagttcca aaactatcta aattatcttt ttgtaaaaaa ggcgagtcaa gtgaaatagc
     1441 cacaacacac tattacgaaa tattaaaaaa cctagaaaaa taatagttat atagataaaa
     1501 catattaaaa aatagtggct ttaaattttt taaagtcact attctatata aatataacat
     1561 tataaaaaat atat
//
LOCUS       DAEPOZ010000392         1566 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.395, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000392 DAEPOZ010000000
VERSION     DAEPOZ010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.395"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(165..1112)
                     /locus_tag="KR440_003675"
     CDS             complement(165..1112)
                     /locus_tag="KR440_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBH0700641.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKAKYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDFVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEAIKKIK"
     gene            complement(1081..1527)
                     /gene="lspA"
                     /locus_tag="KR440_003676"
     CDS             complement(1081..1527)
                     /gene="lspA"
                     /locus_tag="KR440_003676"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBH0700642.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRVIWEYVFNVADVFVVVGTVFLCIYVLFFESKSR"
BASE COUNT          536 a          274 c          146 g          610 t
ORIGIN      
        1 aaagataatt ttttaatcta tcaactcaaa ttatatattt atatttcaaa tttttcaagc
       61 ttttttaaac aaaatacttt aatataagat attttattta aatttcacta aatttattta
      121 cttatataaa aacaaaaaat gatttataag tattatacaa atggttattt tatcttctta
      181 atagcttcct taaaatactc aggtaattct gaattaaatt cgatatattc cttagtggta
      241 ggatgtataa atcccaactt cttagcgtgc aaagtttgtc cttttaattt aaatttagtt
      301 tcttttggac catatacaaa atcacctaaa agtgggtgat ttatagacaa agcatgaact
      361 cttatttgat gagttctacc agtttcaagc ctagctctca tatgtgtaaa cttatcaaat
      421 ctttctacaa cttcaaaatg agtaattgca ttttttccat cctttactac agccattttt
      481 aatctatcct taggatttct ccctataggt ttatcaacag ttattttatc ttctttaacc
      541 acaccatagc atataaattc atactctcta gttatagtat gttcctttaa ctgttctgct
      601 agaaaattat gtgcataatt gttcttagct atcataagaa gaccagaagt atctttgtct
      661 attctatgaa ctataccagg tctaataact ccatttattg acgacaactt atctttacaa
      721 tgatataaaa tcccatttac tagtgtattt tcatagtttc caggagcggg atgaacaacc
      781 atatcctggg gtttatttat tattaagaca tcatcatctt catatacaat atctattgga
      841 atatcttgag gtaaaacctc taatacttta ggttctggta tctctataac aatattatct
      901 tcctctttaa ctagatattt agctttttct actttattat tgacctttac ttttccatct
      961 tttataattt tttgtatata acttcttgac atatcaccta attgttctgc tagataaata
     1021 tcaagtctaa cttcttcttc ctcttcaagt actaaaaact cttttacttc atccattata
     1081 tcacctactt ttactttcaa aaaataaaac atatatacat aaaaacacag ttcctacaac
     1141 tacaaataca tcagctacat tgaatacata ttcccatata actctaaagt cgaagtaatc
     1201 taccacaaaa cctaatcgta ctctatcaat tagatttcct aatgcaccag atataattaa
     1261 tataattccc aaccttccaa atatatgtac tttccttgta ttaagatagt ataatccaaa
     1321 tactgttgca agtaatgcaa caattataaa tatccactga ttattttgta ataatccaaa
     1381 tgctgcacct ctattttcga cataagttaa atgaaataca ttatttatta ttggtattgt
     1441 tgaaacatct accaaattat tcaataccca tattttagac agttggtcta agcctatgag
     1501 tagaattatt attaatatat atagcaatga tttttccccc tataaattat ttaaaaacca
     1561 atttta
//
LOCUS       DAEPOZ010000393         1549 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.396, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000393 DAEPOZ010000000
VERSION     DAEPOZ010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.396"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            165..338
                     /locus_tag="KR440_003677"
     CDS             165..338
                     /locus_tag="KR440_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBH0700643.1"
                     /translation="MHLVQLLCQQINGSDIDPDSKFGPATYNQVKILQGRLGVTKDGV
                     VGKATWQAVGERY"
     gene            complement(423..1445)
                     /locus_tag="KR440_003678"
     CDS             complement(423..1445)
                     /locus_tag="KR440_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBH0700644.1"
                     /translation="MNYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTLLNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNSGNGIVFIKPWNNSKIIEKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTKLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
BASE COUNT          558 a          301 c          194 g          496 t
ORIGIN      
        1 attcatataa tattaatata gatgattata atattgaacc aaaagcattt attattagat
       61 ataaaaaaga tttaaatcgt tttgatgcta ctggtcctat aagttatagc gtaaatataa
      121 ataccactgt agtaggatat ggacatgtag aaaaagggaa tcctgtgcat ttagtacaac
      181 ttttatgtca acaaataaat ggttctgata ttgatccaga tagtaaattt ggacctgcta
      241 catataatca agtaaaaata cttcaaggaa gacttggtgt aacaaaagat ggtgttgttg
      301 gaaaagccac ttggcaagct gtcggagagc gttattagaa aagaggttgt cgcactaaga
      361 aatagggcga caactttttt atatagaata tattaatcag tatcttagag tctagaaata
      421 ttttacaata aattccatat aactctacca tctatatcct taacattttg acaaccagtc
      481 aaaatcatag tctcacataa ctcattccta accttctcta taaaaaactc cacaccatca
      541 agaccaccgc caaaagaagc agtaacaaaa ggtcttccca tcaaaacagc atcagcaccc
      601 aatccaagca tcttaactac atcaacacca gtcctaactc caccatcaac aagtattgtt
      661 atctttccct ttacagactt agctatatct ggaagtacct cacaagtacc aggagtataa
      721 tcaagtactc ttccaccatg atttgaaaca ataatggcac tagcaccaga ctctacagcc
      781 atcaaagcat catctacagt cataattcct tttataataa aaggtaattt agtagattca
      841 accaattctc taatctcatc aattgtctta ggagaaaaag gattttcttg aaattgatta
      901 ttaataagtc cacaagcatc aaggtctaca ccaactgcaa aagcacctgc ttcttcagac
      961 aatctaattt tttctattat ctttgaatta ttccaaggct ttataaatac aattccattt
     1021 ccactattat ctttcaacac atctaaatta tccagtaaaa aagtatctac attagtatcg
     1081 ccaaccatag cataaattcc agaattagaa caacctctaa caacaggctc tatgtattct
     1141 ttttcggaaa ctttaccacc catgttcaat agagtacctg atacaggtgc agcgaatata
     1201 ggagaactca tttttctacc aaataattct atggatgtat ctggctttga aacattatgt
     1261 ataactctca tatttatctt tattttttct agactttttc tattttcaat gaaagacgaa
     1321 ccacttcctt taccacccat accaggaaca tctccagcac aagctaatcc attacaaatt
     1381 ttacatactt tacatgttcc attaaagttc tctctagctg attttagtaa gtcattgtaa
     1441 ttcatacaaa atctctcctt tttataatat taaaattgag tattactata ttgaaaatat
     1501 cttaattgta aatataggga tgttattatg atgagctaaa ctagataat
//
LOCUS       DAEPOZ010000394         1547 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.397, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000394 DAEPOZ010000000
VERSION     DAEPOZ010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.397"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            128..1531
                     /locus_tag="KR440_003679"
     CDS             128..1531
                     /locus_tag="KR440_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBH0700645.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
BASE COUNT          622 a          197 c          273 g          455 t
ORIGIN      
        1 acatcttaca aatttatttt tatttagata acatgtatat tttttccatg aaaaataaat
       61 aataaaaaat agtaaatatg gaaaatataa aattgattaa caataattaa ttacctgagg
      121 tgtgaatatg aaaatgctaa acaacttttt taagaaaatc aacaatccag ctcctgtctt
      181 tgcacagaca aaaatggaaa gagataatca aacagatgat tcactttcta caaaaccaaa
      241 gactacattt agagacgttg ctggattaga cgaagtaaaa gaagaactat ttgaaatagt
      301 cgattttatg aagtcaccac aaaaatatca aaaaatgggt gcaaaaatac ctaaaggagt
      361 attattttat ggtcctccag gaacaggtaa aaccctactt gcatctgctg tagctggtga
      421 aacaaactca tcatttttca atgttactgg ttcagagttc gttgaaaaat atgtaggtgt
      481 gggagcaaaa agagttagaa ctctatttga aaaagctaga aaagaagctc caagtataat
      541 atttatagac gaaatagatg ctgtaggagc aaagagacat cttgaaagta acaacgaaaa
      601 agaccaaaca ctaaaccaac ttttagttga aatggatgga tttaataaag attccaatgt
      661 attaataata ggtgctacca atagacttga cttattagat gaagctttac ttagaccagg
      721 aagatttgac agacacattc atataggtgc accaaactat cacactagat ttgaaattct
      781 aaaggtacat acagatgaca aacctataga caaatctgta aatctagaac ttttagctaa
      841 aaaaactcat ggtttcaatg gggctcatct ttcaaatata gcaaatgaag cagctatatt
      901 tgcagttaga gatgacagtg agtgtattac atcagaacat tttgacaagg cacttgaaag
      961 agttattgca gggctagaat ctaaaaactc tgcattggtt gaaaaagaga aaaaaatagt
     1021 agcatatcat gaagctggac atgctcttgt aagtgatata gtaggaattt gtcctataca
     1081 aaaaatatct atagttccta gaggacaagc attaggttat gttcttcaat tacctgatga
     1141 agatagatat atttatacta aggatgagtt gattggaaaa ataaaaatat tacttgctgg
     1201 aaaggcttca gaagaattaa tatttaatca taaatctact ggtgcaaagg acgacttaaa
     1261 gaaagtcact gaaatagcta accaaatggt ttgtgaatat ggtatgagta acttaggatt
     1321 tatgactatt gatggaaatg ataagacttt cttatgtgat aaggttcaaa aggaagcaaa
     1381 tcgtatagtt gaaatttgtt ataaagagac tttagagatg ttaaaggata atcttgaaga
     1441 tttacattct gtttctaaat tcttatttga aaaagaaact atgactcatg aagagttaaa
     1501 agatttgata ggaaaagaag ctgtaaacta atatagtttt aaaaatt
//
LOCUS       DAEPOZ010000395         1536 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.398, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000395 DAEPOZ010000000
VERSION     DAEPOZ010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.398"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            58..669
                     /locus_tag="KR440_003680"
     CDS             58..669
                     /locus_tag="KR440_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBH0700646.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIIEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            967..1305
                     /locus_tag="KR440_003681"
     CDS             967..1305
                     /locus_tag="KR440_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HBH0700647.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
BASE COUNT          612 a          169 c          196 g          559 t
ORIGIN      
        1 tattattgtt aaaactcaat tatataattt ttaataatat tattttagga ggtaaaaatg
       61 ataaacgata ttaaaaataa attcaaaaat tacaaaccat atataaatgg atacaagaat
      121 atgaagagag catctgtttt aattccaata atagaaataa ataacacaca ctatatatta
      181 tttgaagttc gctcaaaaaa tttgaagcat caacctagcg aaatttcttt tccaggtggt
      241 aaaatagaat caggtgagac tccatatgaa gctgttatta gagaaacttg tgaggaactt
      301 ggaactttta gtgacaatat agaagtaata tcaagtcttg acctccttat aacaccagta
      361 aagtttatca tacatccata cttaggatat ataaaaaata ttaataattt aaatataaac
      421 aaggatgaag ttgaccatac ttttttggtt cctataaaat atcttttaga gaatcctcca
      481 aatacttata gtaatactgt aaaagttttt ccagatgaaa atctaccata tggttcaatt
      541 ccaaaccaaa aagattataa atttgaaagt ggaagttatc ctattctgtt ttataaatat
      601 aaagattatg taatttgggg aatgacagca aaaatactag aaaacttttt aaatttttta
      661 tcagaataac actcataata aatcatcaaa acttttacat aagaaattta taaaagtttc
      721 taaatttaac aattctaaaa tatcaataac tatattttgt agcacttttt tattttatta
      781 ttgtttgaat atttgatggt tttcaatgca atttcaacat aaatactatg tattagtgta
      841 tactgttgat gaattaaagt atgataatct atttaataaa gtatgaagta aatgatttta
      901 atgtgtcttt atggtaaaat tactaataag taataaaatt aattaaaata tatggaggtg
      961 cttataatgt ttgctgtagg atttgttgtt ataattgcac ttgccttttt cattttaagt
     1021 gtactattat ttaatggaaa agccagtatg ctactagcag gttataactc tatgagtaag
     1081 aaagaaaaag cacgatacga taaaaaaaaa ttatgtagaa gttctagcat tgttactttt
     1141 attgtctcta taatgctatt tattatggca tttttgggat atagaataga gagtggtcaa
     1201 atggacgaaa aaaaaatggt tcccttttct ataatattta taatagtcat actttccagt
     1261 gtatttttta atatcctcta ctctaacaaa aaatgtaaaa aataaatagt tgtaagttga
     1321 gcagttaatt ataaaagatt gactgctttt tcatgtatat tgactactct tattaaatat
     1381 tttaacattt aaaaatctat gtaaaaataa tgaatatagt acaaacttct aataatgatt
     1441 ttacctttgt ggttacagtc cactatatat aatttaatat gataacatgg aattactaat
     1501 gacaactaag taagctttta taaaatataa taaaac
//
LOCUS       DAEPOZ010000396         1516 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.399, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000396 DAEPOZ010000000
VERSION     DAEPOZ010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.399"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(247..1374)
                     /locus_tag="KR440_003682"
     CDS             complement(247..1374)
                     /locus_tag="KR440_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBH0700648.1"
                     /translation="MIIPKLEPNDRKREFNKYSSEQINKVVYNYLFKNNMSNRKIDDE
                     ILDLSSKQSKGYQSMGIVHHLGINKNHKGIFTGYTIDKAIEVLKEDEIHFKQIINILL
                     EIRNINEYPIIVDSWELLDEKIALKTIDKSTLKHNGTGVPIEIRYFFEINNLGKNEKK
                     NVNFKYNDNYYSAVIEMDKLENPRSRIIWKSKFRDLLRSIFSEYYEKFLENKIEGKTN
                     LPKIRFIKLDSNNFNIEFIFTAEIEDDVLEEISQDNPLNINTINIELETEIRKEGKVK
                     YVYGKQYERNPRNRIEAIKYHGTKCIVCGFDFEKTYGDRGKGYIEIHHIKPLSSVGEE
                     SNINPKTDLVPICSNCHRMIHRKKDNVLSIDDLKQIIKDSK"
BASE COUNT          476 a          248 c          126 g          666 t
ORIGIN      
        1 tttatttaac aataaaaata catttctatg gattcatcat acatgttagt cttaattaat
       61 tttcatcaat aacttaagat atacctaatc ggttagacta atatacctca aatacttaat
      121 ttataaaagt agctgttata ctttagcaac taccttttcc taataaattt tttgattttt
      181 tatattagta tttgttgtta actagtttag aatatatact ttatacatct ctgccacaca
      241 taagcattat ttactatctt ttataatttg cttcaaatca tcaatactca aaacattatc
      301 tttcttcctg tgaatcattc tatgacaatt tgaacatatt ggcaccaaat ctgttttggg
      361 atttatattg ctttcttctc caacagaact taatggttta atatgatgaa tttctatgta
      421 tcccttacct ctatctccat acgttttttc gaagtcaaac ccacatacaa tacactttgt
      481 gccatgatat tttattgctt ctattctatt tctggggtta cgctcatact gtttcccata
      541 aacatattta actttccctt cttttctaat ttcagtttct aattctatat taatagtatt
      601 aatgttcaaa ggattatctt gacttatctc ctctaataca tcgtcttcta tttcagcagt
      661 aaaaatgaat tctatgttaa aattattact atcaagtttt atgaatctta ttttaggtaa
      721 atttgttttt ccttctattt tattttctaa gaatttctca taatactcag aaaatatact
      781 tcttagtaaa tctctaaact tagatttcca aattattcta cttcttggat tctctaattt
      841 atccatctct ataactgcac tataataatt atcattatat ttaaaattca catttttctt
      901 ttcattcttt cctaaattat ttatctcaaa aaaatatctt atttctattg gaactcctgt
      961 cccattatgt tttaatgtag atttatcaat tgtttttaaa gctattttct catctaataa
     1021 ctcccaagag tcaactatta taggatactc atttatattt ctaatttcta aaagtatatt
     1081 aataatctgc ttgaaatgta tctcatcttc cttaagaact tcaattgctt tatctattgt
     1141 gtatcctgta aaaatacctt tatgattttt atttattcct aaatgatgca ctatacccat
     1201 tgattgataa cctttacttt gttttgatga taaatctagt atctcatcat ctatctttct
     1261 attactcata ttatttttaa ataaataatt ataaactact ttgtttattt gctctgatga
     1321 gtatttatta aactctcttt ttctatcatt aggctctaac ttaggtatta tcatattctc
     1381 ctcctcaatt gattatttta gattttaata aaattattgt atcatatatt tattattaaa
     1441 atatattgtg ctatttatca aaatacgtaa atcctacaat gtcattgatt gcataaaaat
     1501 aaaagagggc atactt
//
LOCUS       DAEPOZ010000397         1482 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.400, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000397 DAEPOZ010000000
VERSION     DAEPOZ010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.400"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            72..248
                     /locus_tag="KR440_003683"
     CDS             72..248
                     /locus_tag="KR440_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700649.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            658..1296
                     /locus_tag="KR440_003684"
     CDS             658..1296
                     /locus_tag="KR440_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700650.1"
                     /translation="MSNQIKDFDITNDMFQESIPITTKTILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLVKAANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYSLIWNQ"
BASE COUNT          624 a          120 c          208 g          530 t
ORIGIN      
        1 tgtcagccag ttgccaaaaa gagatattct ttttgttttt atgaaagttt tttaggggga
       61 taataatata tatgtttaga gataaaatgg atagatgtac acatatgttg actgcttata
      121 ttggtagttc atatgattat tgtgatttta tagatacaca actagatgat tttatactag
      181 agtacggaga aaatgtggta gaatcttgtt tgcatcaagt gatggtatta gtaagtaagt
      241 ataattaaat agatttggtt ttctagagaa ccctttttag ttttatctac tttaaatagc
      301 cttatgttag aaagctaatt aagatagatt tctagtattc ataattttat tttctattta
      361 aaatacatat aataaagatg taaatttgta tcaaataata aatatgagaa cttaaattct
      421 cttatctatg tgaaaataaa catctcttaa aatgaaattc tgtaaacata aatgattttt
      481 tagtttgaag gattagatta acaaaattat aaatttgcaa ataagctata ttaaaaaata
      541 taataatttt ttatataact tattctttta taacttttaa ttttaaaaga ggagaatgta
      601 tataagagta ttcaatgtac tagaaaaaat caaaagaaat attagaagaa atggatattg
      661 agtaatcaaa taaaagattt tgatattaca aatgatatgt ttcaagaaag cattcctatc
      721 acaacaaaaa ctattcttga agacatgcct tcaaatgatg aattaaatca tgttttttca
      781 aaaggttgtg aacgaaaaat gaaaaagaga aaaattgtat taatcacact actattaata
      841 ggtgttttgt tattaggaag tatcctatat aatctttttt tagtaaaagc agctaatatt
      901 tcaatgctaa aggaatcttg gaattttgat ataccaatac ctaacaaaga aattgaagta
      961 tttgatactc aagatagtat taatggagat ggacaaagtt attttattca aggattttct
     1021 gaaaagaatt tcaaaaaagt ttttaattta aaaggtggta tagttgtaag caaagataat
     1081 attaatgaaa tagaaaaata tattgataaa tttaaaagag attctgttaa cattaataaa
     1141 agtaataaaa ataaaattga agaagatttt aaaaaatata agttagaagt taaaaaagat
     1201 gataagtata tatataagag aaattatgaa aactatgtag ttttaattat aaaaaaagat
     1261 gaacaaaaat tatacagttt aatatggaat cagtaggtac atatgtatta ttaagaatta
     1321 taaataataa aaataaattt tagtcaatct acaaaaacct tttaaattgt ctggaaatca
     1381 cctcctcatt tgttataaaa acatacaaca aatgaagagg tgattttatg gtttttaaaa
     1441 aaataattat aagtggatat gtgctttcac atgtttatca ac
//
LOCUS       DAEPOZ010000398         1463 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.402, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000398 DAEPOZ010000000
VERSION     DAEPOZ010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.402"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..>1463
                     /locus_tag="KR440_003685"
     CDS             <1..>1463
                     /locus_tag="KR440_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBH0700651.1"
                     /translation="KFNLPTWEWSKFLEEANYDLSNNINIKKEYNIFDSKEEKKKERE
                     TLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKTIRELFRNSEDEEKIAC
                     IEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLINKDIPNVLDSLGLNGK
                     VSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLKRLVEGGKYGDIEEINY
                     LVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELKEEHITVVNHSLLAKWP
                     YKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEIYPYEFIQNSSFIYKKY
                     SRNMRKIKAFDKFYNVLKIGREDKQKIARSINLIIEEIDSILKFGNCNEYNNVSNYNL
                     RWELNLQIDEIVGKLKKDGIDTEISYRAYSEKIKLSCEKIIKNLVSIIIIIYRNIDDD
                     SIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDD"
BASE COUNT          668 a          108 c          255 g          432 t
ORIGIN      
        1 aaaatttaat cttccaacat gggaatggag caaattttta gaagaggcaa attatgattt
       61 atcaaataat ataaatataa aaaaagaata taatatattt gactcaaaag aagaaaagaa
      121 gaaggaaaga gaaaccttaa aaattttaaa tgaagaagaa aaaaattatg aagaattatt
      181 aaaatacaaa actatttggg agaataaaga aggatttaca tatgaatata gacctggtca
      241 atatgagctt actaaaacaa taagagagtt gtttagaaat agcgaagatg aagaaaaaat
      301 tgcatgtata gaggcaccaa ctggtatagg taaaagtgta ggatatttat tgcctgcaat
      361 attagaggct agaattaata aaaaaagatt attaatttct actgacacaa aggaattgca
      421 gatacaatta ataaataaag atattccaaa tgtgttagat tctttgggtc taaatggaaa
      481 agtaagttac ggatatataa agggaaaaaa taactatatt tgtatagata gactggaagc
      541 atatatagat gattatgagt ctcaaaaccc tactaaagga gaaattttat ctttaatatt
      601 tttgaagaga ttagttgaag gtggcaagta tggagatata gaagaaataa attatttagt
      661 gtttgacaac ttcaaggaaa taagtaccca cttaagaaat gtaagttgtg acccaaatat
      721 gtgcagacct aaaaaatgca aaaaagattg tttatataaa aacagaatag aagaattaaa
      781 agaagaacat ataacagtag taaatcattc actattagct aaatggccgt ataaggatga
      841 aaaaccttta gaaaatataa tagtagatga agcacataat ttgactgaaa aaggatatga
      901 tttcttttca agcattataa attcaaagtc tttaaggtat ttattacaag aaatttatcc
      961 ttacgagttt attcaaaata gtagctttat atataaaaaa tatagtagaa atatgagaaa
     1021 aataaaggct tttgataaat tttataatgt attaaaaatt ggaagagaag ataaacaaaa
     1081 aattgctaga agtataaatc tcattattga agagatagat tcaatattaa agtttggtaa
     1141 ttgtaatgaa tataataatg taagtaatta taatttaaga tgggaactga atttgcaaat
     1201 agatgaaatt gtgggaaaac taaaaaagga tggtatagat actgaaataa gttatagagc
     1261 ctatagtgaa aagataaagt tgagttgtga gaaaataata aaaaatttag tttcaataat
     1321 aataataatt tataggaata ttgatgatga tagtatagat aaagaagcag atatatataa
     1381 atttggaaag gcaaagacaa gagatttaga ggatataaag attatatttg aaatcttttt
     1441 agagtatgat gaaaaggatg att
//
LOCUS       DAEPOZ010000399         1455 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.403, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000399 DAEPOZ010000000
VERSION     DAEPOZ010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.403"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(305..772)
                     /locus_tag="KR440_003686"
     CDS             complement(305..772)
                     /locus_tag="KR440_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HBH0700652.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKSEKWVDVCKNW"
     gene            complement(851..1393)
                     /locus_tag="KR440_003687"
     CDS             complement(851..1393)
                     /locus_tag="KR440_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700653.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDIGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITESTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
BASE COUNT          472 a          256 c          116 g          611 t
ORIGIN      
        1 gtatttttat ctgactattt tttattttta aatatcttac atctataata gtaacactca
       61 aaggataatt tttcaataat ttctaattat ttttttaaca atatttatta cattttttcc
      121 acttttttgt cattttttta ttgtttcgaa aaaaaatcag aattttttaa ctttattttg
      181 gattattagt ttaaattaga ttttcctctt agatttataa atatttacac aaaaaactac
      241 ctctgtatag attgtataca gaggtagttt tttactaatt cctactatag tttactatat
      301 ttttttacca attcttacaa acatctaccc atttttcact cttagaaaac ttctccattt
      361 cttcacatgt aagttctatt gcagagttac tactgccaca tgcaggaaat actgtatcaa
      421 atctcttcat tgaatcatct aaatagactt ttatagaatt ttttaaacca aatggacaga
      481 ctccaccaat agcatgtcca gtaaactcca agacttcttc tggtgtcaac attttagcct
      541 tacaattaaa ttcagcctta tacttcttat tatcaatctt agcatcacca gctgtcacaa
      601 ttaaaattgc atcatctcct attttaaatg agagtgtctt agctattctt gctggaataa
      661 ccccagctgc ttcagctgca agttccacag tagcactaga ttgttcaaat tctaatatac
      721 tgtcttcctt gccaaattgt ttaaaatact ctcttacatt acttattgac attaaatacc
      781 cctcctagtc tgaattttta taaatttcat attattctta attatatcta taattgattt
      841 atttttcaaa ttattccata atattagcat tccatgtatc attatttttt ccaactacta
      901 gataaagtcc ttttgccatt ggaatattga caatatattc tcctttatat tctacactca
      961 taccagattc attaaagtac ctataatcaa aatttagtcc ttttggaatt acaaaatcaa
     1021 cataaccttg attatttggt tttattccat atttcagagt actttcagtt atagaatttt
     1081 tttcattaaa taaatctaca tttacatatg cagaacactt attcattccc tttttatttt
     1141 ccacacaatt ttcaaacttc attttagcac tgtttaattt ataactatat ttactattat
     1201 tgcttatttt aaatcttaca atagtaccga tatctctact tctcctgcta tcaaaatcaa
     1261 aaacaatttc ctctaaatga tttatttcat aatctttgac acttaccata tctctatata
     1321 ctaatttatt tatagctatg aatagtgtaa aaatccccaa aataaaaaat gaaactttta
     1381 taaacttctt catttttagt ctccttatat tttgtgctat tgtgctataa tattaattaa
     1441 aattttaata attaa
//
LOCUS       DAEPOZ010000400         1450 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.404, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000400 DAEPOZ010000000
VERSION     DAEPOZ010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.404"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            508..>1450
                     /locus_tag="KR440_003688"
     CDS             508..>1450
                     /locus_tag="KR440_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HBH0700654.1"
                     /translation="MMNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHT
                     YLSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINNEMVIALMDSDVKRSSLIRM
                     TISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKS
                     GKIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIVAQKTNKNRKTISCIFQL
                     FDKDNCDEDDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKI
                     VSIQSENISYDVYIFALEILLVILIFIIYIACIINNNT"
BASE COUNT          606 a          116 c          210 g          518 t
ORIGIN      
        1 atataattat tatatttata aaaataaaat agtaaaagga tattataata ttttactaga
       61 attattatct ggtaacttaa gttttgagta ttaatctaaa atatatgttt ttatagggtt
      121 taaattttat gtatacaaaa ttaatttgct aactgaatca tataaaaaaa acaaaatatt
      181 ttttagaggg aatgattaga tactattttc tttgttatga aattaattga agagtaatta
      241 attagaaaag gaataaataa taatgaatat attacattat tactttttga taaatcaaaa
      301 ataaagttat agtttttaat attaattaag aatgatgaaa tatgaataaa gattttacaa
      361 ataaatctta agctatctta tttaattcta atgcaggaag aaggtaaatg tatattgaaa
      421 aaatcataga aatagaagta aacaacttaa acaaatacaa aggtttgcaa aagaagtaat
      481 ataattatat acaatgagga tgtgagtatg atgaacagac taaagccaag aattttaggt
      541 attagtatct tgtttattat tataattatt ttaacatgtg gagctgtttt caagtttaaa
      601 tttaaattag ataataaatt aaaagaaacc acacatacat atttgtctga gtttacagaa
      661 cacaatataa ttaatctcaa gacaagactt agtggtcaat ttgacatgct tgaatctatt
      721 gctaacttca taggaaactt gaataatata aataatgaaa tggtgattgc tttaatggat
      781 tctgatgtaa agcgaagttc tttaattaga atgactatct ctacattaga tggggaatgt
      841 tattctaatg atggaatcac ccataatgtt caagatagag attattttaa aaaagctaaa
      901 aatggagaaa agaatatttc agagccttta aaatctattg ttaataataa agaaataata
      961 gttttatcta ccccagttta taaaagtggg aaaatagttg gtgttttaag tggaatttat
     1021 gattcaaaaa agttaaatga gttattagga ttatctgcat ttgatgaaaa agcagatgtt
     1081 tttgttgcaa agaaaaatgg agatatagtt gctcaaaaaa caaataaaaa tagaaagacg
     1141 atatcatgta tatttcagct ttttgacaaa gataattgtg atgaagatga ttgtctaaaa
     1201 actataaaag aaaatatgaa aaatgataag tctggatatt ttaattataa cttagataat
     1261 gaaaaattta tgttaaacta tcagccactt ggagttaatg attggtatgt tttttctgtt
     1321 gtgccaaata aaatagtgtc tattcagtca gagaatatat cttatgatgt atatattttt
     1381 gcattagaaa tactactagt tatattgatt tttataatat atatagcatg tattataaat
     1441 aataacacca
//
LOCUS       DAEPOZ010000401         1446 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.405, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000401 DAEPOZ010000000
VERSION     DAEPOZ010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.405"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(195..377)
                     /locus_tag="KR440_003689"
     CDS             complement(195..377)
                     /locus_tag="KR440_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700655.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMVKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            690..1109
                     /locus_tag="KR440_003690"
     CDS             690..1109
                     /locus_tag="KR440_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HBH0700656.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
BASE COUNT          586 a          124 c          202 g          534 t
ORIGIN      
        1 ttaaattata tcatattaga tagtcaaatt ttgaaaaaaa actaacttat ttaagtatac
       61 attttgaaaa aattaaaaaa gctatatata gatttttaaa gaaatctata tatagctttt
      121 aagtttatgt caatcttttt ttattaatga ataaataata tagaatacaa tatacaaaaa
      181 agtttcttaa aatgctaaga taatccccat ttacaaagca acatcattat actgtgtaaa
      241 tttgaatcaa caatgttttc accatatttc accataaaat cttctaattg tgtgtcaata
      301 aatgaataat atccacttat actatcgatg tatgcagtca ttaagtgtat acattcatca
      361 cttttatctt tatacatata tatctccccc tatatacaat atataaattt agatactaaa
      421 tcgtatcata ttaaatagtt aagtgtgtaa catctatgca gttatatatt tatatcctta
      481 tattaataac taaagtgagt tttgacaata gattttggga aactggatgt tcaaagtgta
      541 aacttggatt ttaaaatcat taattaagat agtgtatagg aatatatgta tacataactt
      601 aattataaag attataaaca attactttta ttaactttat ttaagtggta gaataacttg
      661 gagattaata attataagga ggatataata tgagtttata caagtttata tatgatgata
      721 aggaatattt attaaaagag gagaactgta gtgctttaat aaatgatgaa gaaaatccag
      781 ttcaaggagt aagtatttca aaaataattg atatacttaa tgaagcagaa gaagttgatt
      841 ttgatgtaga atattatcaa gaagcatgtc cattatgttt agaaggagta aaagaaaaga
      901 aaaaattctt tcctttttta gaatatcact tttatatttt cagtaaagat ggagaatatg
      961 taataagcaa tatatctgtt gattataaag gactatcttt taataaacta tcaagggcaa
     1021 ataaagttga taatagttat attgtaagtg tgataatttg tgaaaattgt caggattata
     1081 tagttcagat agaaaattgt atagtttagt gtaaagtatg tcttaattta agagaaattt
     1141 ctaaattgag acatcaattt ttttcaaaaa tagtatggaa aattactaaa tttatgagat
     1201 aatgtaagtc ggaggtgagt tacaatatat agtaaatatt tggaagagat agatgataca
     1261 attttagatt caatgtttaa actaaccttg aatgaaagat tattgactaa taacgatttt
     1321 gttgaaaata aaattggtgt agtaaagaat ttaattgatt gctataaaac tgaacaaaat
     1381 agtttaatgt tattaaaatc aaattttttg ctagctttat tatatggtat tcaagggttt
     1441 agtgaa
//
LOCUS       DAEPOZ010000402         1421 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.406, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000402 DAEPOZ010000000
VERSION     DAEPOZ010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.406"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(124..342)
                     /locus_tag="KR440_003691"
     CDS             complement(124..342)
                     /locus_tag="KR440_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0700657.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
     gene            complement(343..750)
                     /locus_tag="KR440_003692"
     CDS             complement(343..750)
                     /locus_tag="KR440_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700658.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            complement(836..1324)
                     /locus_tag="KR440_003693"
     CDS             complement(836..1324)
                     /locus_tag="KR440_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HBH0700659.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
BASE COUNT          498 a          242 c          123 g          558 t
ORIGIN      
        1 aagtcattta ttctctaatc tataagcttg gttttttacc tataattaat tcattctcta
       61 atacataaac ttgaattttt tatctattaa ttcattctct aatgcataag taatacatga
      121 aatttaaata ttattttctt caataaattc aaacaactct tctacagttg tatcaaatac
      181 cttagcaata tccattgcca gttttaaaga aggattgtag cgaccatttt ctaaatgtac
      241 aatagtttca cgtcttactc ctactgactt tgcaagttca ccttgtgtca taagattctt
      301 ttctctatag gatttaattt ttgtctttag taatactgac acctaattgt atatcccctt
      361 tctgtcaaaa tatataaata gaattaactt caacaaggat tgaataaaca aaatgactaa
      421 taaagacatt cctatattta aatttgacac atttatgtcc aaagttacaa gaaatataga
      481 tactatagct acagaaaaaa acaatacttt tattgcaata tcattaactc ttgatagtat
      541 acattttgat gcctcatcaa ttaaatcatc atacttttta ttatagaccc actgagctcc
      601 catagctaat acccaaataa ttataggaag atattcaata ctacttcctt taaaaaaata
      661 cttatttact aatgtagctc caatacaata agaaattccc aatatttctg aaataagtgt
      721 tgatttaata ccagtttgtt gtttagacat ataaaatccc ccttaaaaag ttatgtattt
      781 ataacacctc ttgttataaa tatataactt ttttgaataa atttcaataa aaatattatt
      841 ttgcaacaaa ttcttttatt aattttacat tagatagagg cactttagat gtcactcctt
      901 gcaatgtcaa atcttctata ttaacttcat ttaattttga acacagttct aagtcaaata
      961 ttctttccca acttaccttc ttttcaattt gttcatcttc ataaagctct acagtaatat
     1021 ccattaaagg taaatgccat aaatcaaatt ctgataataa cacattgtct tcacttattt
     1081 ctatttcaag taaaacacct ttttttcctt tttctaaata accactacat cttaaatcag
     1141 gtctcttcgt ccaaacccat atcatactat catcaaattc atatattcta tttgtcattt
     1201 gactatacat ccatttgtat ggaatatcaa agtcttctaa tataaaagaa taatcatttg
     1261 atagatattt attgttttta ttgttgttcc aaaattctaa agtctgtata gtataaaatt
     1321 tcatatataa atcaacccct ttgtttttct ataagaagga gatagttcaa acttttattt
     1381 tgaatcatct ccttagctta tatatctctt tgtatctact a
//
LOCUS       DAEPOZ010000403         1409 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.407, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000403 DAEPOZ010000000
VERSION     DAEPOZ010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.407"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            79..588
                     /gene="vanZ1"
                     /locus_tag="KR440_003694"
     CDS             79..588
                     /gene="vanZ1"
                     /locus_tag="KR440_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ1"
                     /protein_id="HBH0700660.1"
                     /translation="MKSRKHNITKGLFIVYIIILTWIILFKLQFDISSLETMNLRSIN
                     LVPFAGSLIINNRVDISEIILNVAIFVPFGIYVCMLKEEWSFIKKVIPIFITSLAFET
                     LQYIFALGASDITDLIGNTLGGIIGIAVFMLLSKIFKNNTIKIINVLALIVTIIVVLF
                     LGLVIFANL"
     gene            complement(690..884)
                     /locus_tag="KR440_003695"
     CDS             complement(690..884)
                     /locus_tag="KR440_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HBH0700661.1"
                     /translation="MSVIVMCSRCGKEIDKYRNKYITYYEKNTNAEIKVCINCALDLL
                     EKTRENQELNIREFQDTIIL"
BASE COUNT          549 a          137 c          174 g          549 t
ORIGIN      
        1 aaatagttga cagataaatt accttactat attattgtat taataaaata atacaataat
       61 atagtaagga ggtaataaat gaaatctaga aaacataata ttacaaaagg attatttata
      121 gtttacataa ttattttaac atggataata ctatttaagt tgcaatttga tatctcatct
      181 cttgaaacca tgaatcttag aagcataaac ttagtacctt ttgctgggtc acttataatt
      241 aataatagag tagatatatc agagattata ttaaatgtgg ctatatttgt accatttggg
      301 atatatgtgt gtatgctaaa agaagaatgg agctttataa aaaaagttat acctattttt
      361 ataactagtt tagcatttga aacacttcag tatatatttg cactaggtgc aagtgatata
      421 accgatttga taggaaacac actaggagga atcataggaa tagctgtttt tatgctatta
      481 tcgaaaatat ttaagaataa taccataaaa attatcaatg tattggcatt gattgtgaca
      541 attattgttg tattattttt aggattagtt atttttgcaa acttatagaa ataagtatat
      601 aggtttagct taaaatagaa cgtaagatac agttacatat aaaattattt acacataaaa
      661 agcacctata taggtgcttt cctaaattat taaagaatta ttgtatcttg aaactctcgt
      721 atgttcaatt cttgattttc tcttgttttt tctaataaat ctaatgcaca atttatacaa
      781 actttaattt ctgcattggt atttttctca taataagtta tatatttatt tctatattta
      841 tctatttctt ttccacaacg actacacatt acaattacgc tcatatctta gttcctccat
      901 ctctaattat tcttaatata ttcaatagta tcacttttct gaaaataatg attgttaaaa
      961 ctgtatcaaa tttagattta ctttttatta taatgcattt taataaaaaa ataaaggtta
     1021 tgggtaaaat atttatagaa aaaagtatat tattgaatag attataaaat tttattattt
     1081 ttcatcagtc ttaaatacac attaatatct ataaaatgac aaaataaaaa atactattta
     1141 aatagagatt aaaaaattga tgcgaagtat ttgtttgcta tatgaataac aatttattta
     1201 gaagttttga tttgacactg tataataata aagtaattgc taaaaaagtt tgatgatttg
     1261 gatgtatttt tagaaattag aaaaatatat acttactgac taatgataat cttatatata
     1321 ttttagtttg agtgatttaa gaaaagtgtt ttgaaagggg attattttat agaataaata
     1381 tatatatata gtagaaaatt acatataaa
//
LOCUS       DAEPOZ010000404         1402 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.408, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000404 DAEPOZ010000000
VERSION     DAEPOZ010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.408"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            87..1238
                     /locus_tag="KR440_003696"
     CDS             87..1238
                     /locus_tag="KR440_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HBH0700662.1"
                     /translation="MPIKYWLPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSV
                     GILYINFIVFYLFKFIIDKTKLSMKYEILEKEIIHKEELRLSNECYKIIVEQTDSVVF
                     EWNIKENKSFVSQAWTEKFGYNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNI
                     HSQAVYRLKKSNGEYIWCRTSITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAES
                     DSLTNIYNKGTFEKLVEETIVMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTF
                     ADKIQASFGNKDLVGRIGGDEFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFD
                     SSVSIGIARYPQDGTSFFELFKNADRALYSIKASGKNSYCLFEEELYVQ"
BASE COUNT          527 a          119 c          231 g          525 t
ORIGIN      
        1 gagtattggc gacaatttta tctaaaattg tctttttgtt gacttgtaaa attatttgtc
       61 tatttaaaaa agatgtacat ttagatatgc ccataaagta ttggctccct ttatttttaa
      121 tacctatatt tagtttattt ctgtctgttt ctatctttga tgtaagtaaa ttttttagtc
      181 ttgaaagttt aaaatttcta agtttaatat ctagtgttgg cattttatat attaatttta
      241 ttgtatttta tttatttaaa tttattatag ataaaactaa actatctatg aaatatgaaa
      301 tacttgaaaa agaaataatt cataaggaag aattaagact tagtaatgag tgttataaaa
      361 ttattgttga acagactgat tctgtcgttt tcgaatggaa tatcaaagaa aataaatcat
      421 ttgtatcgca ggcttggact gaaaaatttg gatataacaa tgcatgtaag aatattttta
      481 aagaaattaa agataaagac ttagttcatt ctgaagataa agctatattt gaaggttttc
      541 ttgaaagtat taaaaaaaag aatatacaca gtcaagcagt atatcgttta aaaaaatcta
      601 atggtgaata tatttggtgt agaacatcaa ttacaagcat ttataatgat gaaaatgaac
      661 ttttaagagt tgtaggagta attgtagacg tagatagtga tataaaaaaa tatgaagagt
      721 taagaactag agcagaaagt gactcattga ccaatatata caataaaggt acatttgaaa
      781 aattagtgga agaaactata gttatgaata caggtgataa aaaagatgca ttattcatta
      841 ttgatttaga tgattttaaa gaaataaatg ataattttgg tcatccattt ggtgattttg
      901 tactaaaaac atttgctgat aagatacaag ctagttttgg taataaagac ttagtcggaa
      961 gaattggtgg agatgaattt gttgtatata tgcaagatta tgttacagaa gtaaatttgc
     1021 ataaaaaagc taaagaatta aatagagtac tttcggataa ttacactgat ttgagtttta
     1081 gttttgattc ttctgtcagt ataggtattg caaggtatcc tcaagatgga acttcatttt
     1141 ttgaattgtt taaaaatgca gatagagcac tttatagcat aaaagcatca ggtaaaaatt
     1201 catattgttt atttgaagaa gaactatatg tacagtgaat ttgaatttag attttatgtg
     1261 agagtaagat ttatatatac ttgtggctgt ctgatttata aaatatgtta ctttgtatat
     1321 ttttatatgt ttcagtaagg ttaattaaca taagcaaagg tgtaagtaaa tttatattag
     1381 tttaagttta cttacacctt ta
//
LOCUS       DAEPOZ010000405         1395 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.409, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000405 DAEPOZ010000000
VERSION     DAEPOZ010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.409"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..1324
                     /locus_tag="KR440_003697"
     CDS             <1..1324
                     /locus_tag="KR440_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBH0700663.1"
                     /translation="DGEINTYNSKVKDILDINEDIYGKYIDDIFVDSLKVLLSEKEIL
                     DKVVVFNKKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYT
                     FKDIKTNSPKMFECIDLAKKVSKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIA
                     VNCAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTK
                     LLRVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIP
                     PLRERKEDILIIMEDLINKKLVITPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIV
                     LEKDLPPNLRSSDNKNTSLKLKYSKNDILNILEILILNKESDVGIGRGLILKALLDKN
                     LQITEGKIKKIFEYLKKEELIICSSGRYGSKITQKGEDFYNKLKYKGL"
BASE COUNT          629 a          146 c          212 g          408 t
ORIGIN      
        1 agatggtgaa ataaatacat ataatagtaa agtaaaagat attttagaca taaatgagga
       61 tatatatgga aaatacattg atgatatatt tgtagatagt ttaaaagttt tattgagtga
      121 gaaagagata ctagataaag ttgtcgtatt caataagaaa tatataaatg taaataaaaa
      181 aaacatatac aatagggatg aaaaaatggg cacatattat agtttacaag aaataacata
      241 cataaaaaaa ttagaacaaa atctcactaa aaaattaaga gaaaaaggac aaatagcaaa
      301 atatacattt aaggatataa aaacaaatag tcctaaaatg tttgaatgta tagatttagc
      361 taagaaggtt tctaaatctg acttaagcat acttatcaga ggtgaaagtg gaactggtaa
      421 agaactcata gctcagtcta tacataacaa ctcaaataga aaaaatcaac cttttatagc
      481 agtaaattgt gcagccgttc ctgaaaattt attagaaagc cagctattcg gatatgacaa
      541 gggaactttt actggaggtc taaaagatgg aaaacaagga ttatttgaac ttgctaataa
      601 tggaacaata ttcttagatg aaataggcga tatgccttta gaattacaaa caaaactact
      661 tagagttctt caagagaagc agataatgcc tgttgggtct cacaatgtaa ttaatataga
      721 tgttagaatc atatctgcta caaataagaa tttagaacaa atgattgata atagccaatt
      781 tagagaggat ttatattatc gattaaatac aattccaata aatatacctc ctctaagaga
      841 aagaaaagaa gacatattaa ttattatgga agatttaatt aataagaaat tagtaatcac
      901 accagaagca aaaaaactta tacaaaacta tatgtggaaa ggtaatataa gagaactcca
      961 aaatgttaca tcctatttaa acattatgtg tgaagatatt gtattagaga aagatttacc
     1021 tccaaactta aggtcttcag ataacaaaaa tacatcatta aaactaaaat atagtaagaa
     1081 tgatatattg aatatattag agatactaat tttaaataag gaatcagatg ttggtattgg
     1141 taggggatta attttaaaag cactactcga taagaacctt caaattacag aaggaaaaat
     1201 taaaaaaata tttgaatatc taaaaaaaga agaacttata atttgtagtt caggaagata
     1261 tggaagtaaa ataactcaaa aaggagaaga tttttacaat aaattaaaat ataaaggtct
     1321 ataaaattat aatttatcaa acaaagaaat ctattcagga ttaaagtact ccttcaatag
     1381 gtttttttat ttttc
//
LOCUS       DAEPOZ010000406         1386 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.410, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000406 DAEPOZ010000000
VERSION     DAEPOZ010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.410"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..1315
                     /locus_tag="KR440_003698"
     CDS             <1..1315
                     /locus_tag="KR440_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HBH0700664.1"
                     /translation="KKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLK
                     DIKYILLHELQHFKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLK
                     KLDKDSHIEYGQTLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLK
                     FKSILIFTLIALIVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYD
                     LKSKQYHIYNKEKSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWN
                     EDQDLFSAMENSVNWYFQTLDKKTGLKNIQTYLKQIGYGNYDVSGGISNFWLESSLKI
                     SPIEQVELLESFYTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGW
                     FIGYVEKNENTYFFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
BASE COUNT          564 a          174 c          189 g          459 t
ORIGIN      
        1 taaaaaagat attggtataa ataaggattt tatactatgc aaatcaccta ttataaatac
       61 acctattaca tttggatact ttaaaccaca cattatatta ccagataagt ctatatctaa
      121 attatcttta aaagatataa aatatatctt acttcatgaa ttacaacact ttaaaaataa
      181 agatatactg ataaattata taatgtgctt ccttcaaata ttatattggt ttaatcctct
      241 tgtatggtat gcatttaaag aaatgcgtat agaccgtgaa attgcatgtg atatttcagt
      301 tttaaaaaag cttgataaag atagtcatat agaatatgga caaactctaa taaactttgt
      361 ggataatatt tcaagaccat caaatcttac acttacatca gatattggtg ggtcaaaaaa
      421 acaaataaaa aagcgactat tgaagataat ggattttcat aatgaatcaa atacgttaaa
      481 attcaaaagc attttaattt ttacattgat agctttaatt gtatttggaa atattcccaa
      541 actatctatt atggcagata tcaacgaaaa atatagacct acaaatgaac aaactgtgta
      601 cgaggactta agtcagtatt ttaaaggttt tgatggaagt tttgttatgt atgatttaaa
      661 atccaagcaa tatcatattt acaataaaga aaaaagtaaa acaagaattt ctccaaattc
      721 tacttataag atttatagtg ccttattggg attggaaaat gggataataa caagaaataa
      781 ttcacatatc ccttgggatg gaaagcatca ttctaatagt tcttggaatg aagaccaaga
      841 cttattttct gctatggaaa actccgtaaa ctggtacttt cagactctag acaaaaaaac
      901 agggctaaaa aacatacaaa cttatcttaa gcaaattgga tatggcaatt atgatgtttc
      961 aggtggtatc tcaaactttt ggctagaatc ttcattaaaa atatcaccta ttgaacaggt
     1021 agaattactt gaaagttttt atacaaataa gtttaaattt gaagataaaa atatagaaac
     1081 agtaaaagat tcattgttaa tatcaaaaaa ggatggggta tcactttttg gtaaaacagg
     1141 cactgctaat ataaatggaa aagatgtaaa tggttggttt ataggttatg tggaaaaaaa
     1201 tgaaaatact tatttctttg ctacaaatat tcaaagcaaa gattattcta atggtagttc
     1261 agcatctaag ataacattat ccatattaaa tgacaaaaaa atctataatc cttaatatat
     1321 ttactgtaca ttctaaaaaa ttcacataat tttcatatta attatcgatt aaattcatat
     1381 acaagg
//
LOCUS       DAEPOZ010000407         1382 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.411, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000407 DAEPOZ010000000
VERSION     DAEPOZ010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.411"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..579
                     /gene="rgbR"
                     /locus_tag="KR440_003699"
     CDS             <1..579
                     /gene="rgbR"
                     /locus_tag="KR440_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HBH0700665.1"
                     /translation="SNLKDIDILLLDIKMEKLNGMDTARKIREVDDEMEIIFVTSLID
                     YVQEGYEVRAYRYLLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEI
                     KYIEVQKKDMLIHTINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNA
                     AILESGEEVPISRYRYKEVKEKFLKFLGDTIC"
     gene            573..>1382
                     /locus_tag="KR440_003700"
     CDS             573..>1382
                     /locus_tag="KR440_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBH0700666.1"
                     /translation="MLNFMNELLVVLSIFIQFSIMNGILIYKKDSKKNEKIVFYIVLF
                     LIYIIIQISRLFNGGSYFSNINYLFYILAFIAWGGYYNYFYKMDYIKYSIFFYLFSVY
                     YKCLDAVIYRILFFAITGNVISSKESLLVPHLESIKIQIYSNLIIILIYLVFSFFRKS
                     IKLISQDKRNYVYLLFALLANTMNMLVNYVLKKLERFGSLHSEGYYFDNFVNPKLVGA
                     SSIFLILLFKEIIKENRLKSQAELIKNKLDMQYAHYLSIQESHMRVKKLYHD"
BASE COUNT          573 a          106 c          202 g          501 t
ORIGIN      
        1 agtaatttaa aagatataga tatattactt cttgatataa aaatggaaaa attaaatgga
       61 atggatactg caagaaagat tagagaagta gacgatgaaa tggaaataat atttgtaact
      121 tcattaatag attatgtgca agaaggttat gaggtaagag cttataggta tttactaaag
      181 ccaatagagt tggaagaact aaaaaaacat gtgctaactt gtatcaaaga tattgaaata
      241 aataaggaaa gtcatattac aataaaaaat aagtctaata catataagat ttatttaaat
      301 gaaataaaat atatagaagt tcaaaaaaaa gatatgctaa tacacacaat aaacaagaat
      361 tttgatataa aatatagttt aggtaaaata gaaaaagaat taaatccata taaatttata
      421 agatgccata aaagttttat agtaaattta agatatgttg aaaatataaa gcctaatgct
      481 gcaatactgg aaagtggaga agaggtacct attagtagat atagatataa agaagttaag
      541 gagaaatttt taaaatttct tggtgataca atatgctaaa ttttatgaat gagctattag
      601 tagttttatc tatatttata cagttttcta taatgaacgg aattttaatt tataaaaagg
      661 atagtaaaaa aaatgaaaaa atagtattct atattgtctt gtttcttatt tatattataa
      721 ttcagataag tagattattt aatggaggaa gttactttag taatataaac tatttatttt
      781 atatattagc atttattgct tggggaggat actataatta tttttataag atggattata
      841 ttaagtattc cattttcttt tatctattta gtgtatatta taaatgtttg gatgctgtta
      901 tatatagaat actatttttt gcaattacag gtaatgttat ttcatctaaa gaatccttat
      961 tagtacctca cttagaatct ataaaaattc agatatatag taatttaata attattctta
     1021 tatatcttgt atttagcttt tttaggaagt ctataaaatt aattagccaa gataagagaa
     1081 attatgtata tttattattt gccttattag ctaatacaat gaatatgctt gtaaattatg
     1141 tattaaaaaa attagaacgg tttgggtcat tacattcaga aggatactat tttgataatt
     1201 ttgttaatcc aaagttggta ggagctagta gtatatttct tatattattg tttaaagaaa
     1261 taataaaaga gaatagatta aaatctcaag ctgaattaat aaaaaataag ttagatatgc
     1321 aatatgcgca ttatttaagt attcaagaat ctcatatgcg agttaagaaa ctatatcacg
     1381 at
//
LOCUS       DAEPOZ010000408         1336 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.412, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000408 DAEPOZ010000000
VERSION     DAEPOZ010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.412"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            27..101
                     /locus_tag="KR440_003701"
     tRNA            27..101
                     /locus_tag="KR440_003701"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="KR440_003702"
     tRNA            116..200
                     /locus_tag="KR440_003702"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            209..292
                     /locus_tag="KR440_003703"
     tRNA            209..292
                     /locus_tag="KR440_003703"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:243..245,aa:Leu,seq:tag)
     gene            322..398
                     /locus_tag="KR440_003704"
     tRNA            322..398
                     /locus_tag="KR440_003704"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Arg,seq:tct)
     gene            406..481
                     /locus_tag="KR440_003705"
     tRNA            406..481
                     /locus_tag="KR440_003705"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:439..441,aa:Gln,seq:ttg)
     gene            570..658
                     /locus_tag="KR440_003706"
     tRNA            570..658
                     /locus_tag="KR440_003706"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:604..606,aa:Ser,seq:tga)
     gene            662..737
                     /locus_tag="KR440_003707"
     tRNA            662..737
                     /locus_tag="KR440_003707"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:695..697,aa:Phe,seq:gaa)
     gene            744..817
                     /locus_tag="KR440_003708"
     tRNA            744..817
                     /locus_tag="KR440_003708"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:778..780,aa:Met,seq:cat)
     gene            832..908
                     /locus_tag="KR440_003709"
     tRNA            832..908
                     /locus_tag="KR440_003709"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:866..868,aa:Ile,seq:cat)
     gene            932..1008
                     /locus_tag="KR440_003710"
     tRNA            932..1008
                     /locus_tag="KR440_003710"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:966..968,aa:Pro,seq:tgg)
     gene            1016..1092
                     /locus_tag="KR440_003711"
     tRNA            1016..1092
                     /locus_tag="KR440_003711"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1050..1052,aa:His,seq:gtg)
     gene            1101..1176
                     /locus_tag="KR440_003712"
     tRNA            1101..1176
                     /locus_tag="KR440_003712"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1134..1136,aa:Lys,seq:ttt)
     gene            1184..1257
                     /locus_tag="KR440_003713"
     tRNA            1184..1257
                     /locus_tag="KR440_003713"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1216..1218,aa:Cys,seq:gca)
     gene            1264..1335
                     /locus_tag="KR440_003714"
     tRNA            1264..1335
                     /locus_tag="KR440_003714"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1296..1298,aa:Asn,seq:gtt)
BASE COUNT          320 a          310 c          355 g          351 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atgtaaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaatgcgc ggatgtggcg gaattggcag acgcactaga
      241 cttaggatct agcgccattg gcgtgggggt tcgactccct tcatccgcac catttttgtt
      301 tgatatacat atataaattg tgggtgcata gctcagctgg atagagcaac gcccttctaa
      361 ggcgtgtgtc cggggttcga atccctgtgc gctcaccaca tatattgggg attagccaag
      421 tcggtaaggc acatgacttt gactcatgta tgcgtaggtt cgagtcctgc atccccagcc
      481 aaatgatgta ttactaagct atatttgaac tatttttatt ttaaatatag ttaggttcaa
      541 ttttagaata agtttaagta tccgaatgtg gagaggtgtc cgagtggttt aaggagctgg
      601 tcttgaaaac cagtgacttc gaaaggggcc gtgggttcga atcccaccct ctccgccaaa
      661 tgcccagata gctcagtcgg tagagcaggg gactgaaaat ccccgtgtcg gtggttcgat
      721 tccgcctctg ggcaccaaaa tatggcggta tagcttagtt ggctagagcg ttcggttcat
      781 acccgaaagg tcataggttc gactcctatt accgctacta aaatttaatg tgggccatta
      841 gctcagttgg ttagagcgcc cggctcataa ccggtaggtc tggggttcga gtccctgatg
      901 gcccaccaca ttaaattaat taatataaat tcgaggtgta gcgcagtttg gtagcgcaca
      961 tggtttggga ccatggggcc gggggttcga gtcccttcac ctcgaccaat ataaggtggg
     1021 tatagctcag ttggttagag cgccagattg tggctctgga ggtcgtgagt tcgactctca
     1081 ttatccaccc caattcatat ggtctattag ctcagtcggt agagcacctg acttttaatc
     1141 agggtgtccc gcgttcgagt cgcggataga tcaccaaaaa ggtggcgaca tagccaagtg
     1201 gtaaggcagt ggactgcaac tccttgatcc ccagttcgaa tctgggtgtc gcctccaaaa
     1261 tattccaagg tagctcaacg gtggagcaac cggctgttaa ccggtaggct gtgggttcga
     1321 gccccaccct tggagc
//
LOCUS       DAEPOZ010000409         1331 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.413, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000409 DAEPOZ010000000
VERSION     DAEPOZ010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.413"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            5..961
                     /locus_tag="KR440_003715"
     CDS             5..961
                     /locus_tag="KR440_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700667.1"
                     /translation="MQSQNKKIKQKYYLTKDGWLLGNFAKLLSDYHIKSYSKAINNLS
                     KLNRKKDFYFVSLPHKTNMLKHLYPSGIEGKNNIDINKENLEKQLTDINFLDIDDYFY
                     RSFTEKEREKFFLKTDHHGNSLGAFEEYKFILDKLDLGLSKSEIDSHLKRYSTRYVND
                     KVFIGSYNKKMGYPLKKKESISYVEISDANYKYFISNGTKNTEINENIIKAKLKDKRE
                     WDYGGAYMRGCDCHILKIRNSSSLTNKKALIFRDSYQAPMTWMLADTFKELEILDPRY
                     IKNIDMTYDEIIKNSNANVVLFMYNSFGFAGMIQEMIDKGIA"
BASE COUNT          556 a          156 c          182 g          437 t
ORIGIN      
        1 tattatgcag agtcaaaata aaaagattaa acaaaagtat tatctaacta aagatggatg
       61 gctattaggc aactttgcta aattattatc tgattatcat ataaaatctt actccaaagc
      121 cattaataac ttgagtaagt taaatagaaa aaaagatttt tactttgttt cacttcctca
      181 taagacaaat atgcttaagc acttgtatcc aagtggtata gaaggtaaaa ataatataga
      241 tattaacaaa gaaaacttag aaaagcagtt aacagacata aatttcttag atatagatga
      301 ttacttttat agaagtttta ccgaaaaaga gagagaaaaa ttctttttaa aaacagacca
      361 tcacggaaat tcattaggag catttgaaga atataagttt attcttgata aattagacct
      421 tggcttatct aaatcagaaa tagatagtca tttaaaaaga tacagtactc gatatgtaaa
      481 tgacaaggtc tttataggta gttataataa aaagatggga tatccactta agaaaaaaga
      541 gtctataagc tatgttgaaa taagtgatgc aaattacaaa tattttatta gtaatggtac
      601 taaaaatact gagattaatg aaaatatcat aaaagcaaaa ttaaaagata aaagagaatg
      661 ggactatggt ggagcttata tgagaggttg tgattgtcac atacttaaga ttagaaattc
      721 aagctcttta actaataaaa aggcccttat atttagagat tcgtaccaag cacctatgac
      781 ttggatgttg gctgatactt ttaaggaact cgaaatacta gatcctagat atataaaaaa
      841 tatagatatg acctatgatg agataattaa aaacagtaat gcgaatgtag ttttatttat
      901 gtataatagc tttggatttg ctggaatgat acaagaaatg attgacaagg gaatagcata
      961 acaatttgaa tcagctcctt tttatacaaa aaatagttct caataattcc taatctatat
     1021 gataatttag tacatctctt taacaataaa aataaaatca caaaaattct tataccatca
     1081 cttatcttta tgataaattg gttgagaaaa aattttataa tttatatgat atgcaatctt
     1141 aattaattct ttatctactg caacctttaa taaaagaaac catccaagac tattaatatg
     1201 aatttatata aaaattactt tcaaataaag tactgcagta tttttattta aagtatctat
     1261 aatctttaac cacaaaaagc cacaaaatac aatgtatact tatggctttt tataaattta
     1321 tataattgta t
//
LOCUS       DAEPOZ010000410         1306 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.414, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000410 DAEPOZ010000000
VERSION     DAEPOZ010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.414"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..>1306
                     /locus_tag="KR440_003716"
     CDS             <1..>1306
                     /locus_tag="KR440_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBH0700668.1"
                     /translation="DILNKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIK
                     SSELDTINKEFNMQNENLKNVNNRHKELSINLQDSISEHNKLEDEIQKSKYNLNGYNS
                     KLNVYIDMENHYEGFNRGVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQ
                     NIITDNEFSAKSAINYLKQNNLGRVTFLPLNIIKSNKVSLGNLKANTKFIGIASDLIT
                     FDEKYRNIIENILGRTILINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLK
                     TNGNILSRKRYINEYTEKISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLE
                     KSIIIKSTSIKNVESEIESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDL
                     YNKNKEKIDALNEEIKRYNDLYDKEKSEFDELNLSLVKKTEAY"
BASE COUNT          635 a          100 c          206 g          365 t
ORIGIN      
        1 gatatattaa ataagaaaca agagtttagt aataagctgt ccactttaaa cgcaaataaa
       61 gaaaatatga atatcagaga tgaaaatata aattcagaga taacagagtt aaacaaaaat
      121 attgaaataa aatcatctga attagatact ataaataaag aatttaatat gcaaaatgaa
      181 aatttaaaga atgtaaataa tagacataaa gaattatcaa ttaatttgca ggattcaatt
      241 agtgaacaca ataaactaga agatgaaata cagaaaagta aatataattt aaatggatat
      301 aattcaaagt taaatgtcta tatagatatg gaaaatcatt atgaaggatt taatagagga
      361 gtaaaagaag tactaaaaaa taaaaattta aaaggagttc atggagcatt aggacaaatt
      421 ataaatgtac ctgaaaaata tgagaaatct atagaagcag ctcttggtgc ttatatgcaa
      481 aatataataa ctgataatga gtttagtgct aagtctgcaa taaactatct aaaacagaat
      541 aatttaggta gagtaacatt tcttccatta aatataatta aatcaaataa agtaagtcta
      601 ggaaatttaa aagcaaatac taagttcata ggaatagcaa gtgatttaat tacatttgat
      661 gaaaagtata gaaatataat tgaaaatata cttggaagaa caatacttat aaataatata
      721 gatgagggca taaaatttgc aaaagaaact ggtcataggt ttaaaatagt aactttagat
      781 ggtgaaatct taaatccagg aggttctttg actgggggaa gtctaaaaac taatgggaat
      841 atactatcga gaaaaagata cattaatgaa tatacagaaa agataagtaa tatcaagaat
      901 gaaatttcac atctagaatt aaaaagagaa tctttagaca aagatgtgaa aaatatcaaa
      961 aatgaaattg attcacatga aagtaaaata aaagatttag aaaaaagtat aataataaaa
     1021 agtacaagta taaagaatgt tgaatctgaa attgaatcgt taaaaggtag tattactaaa
     1081 ttagaaaatg agaagaatga tttaaattct aatctaaatt atacacttga aaaaagcgat
     1141 gatgttagaa aagatatgga agagttagat gatttataca ataaaaataa agaaaaaatt
     1201 gatgcattga atgaggaaat aaaaagatac aatgatttat atgataaaga aaaatctgaa
     1261 tttgatgaat tgaacttaag tttagtgaag aaaacagaag cttata
//
LOCUS       DAEPOZ010000411         1296 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.415, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000411 DAEPOZ010000000
VERSION     DAEPOZ010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.415"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(388..588)
                     /locus_tag="KR440_003717"
     CDS             complement(388..588)
                     /locus_tag="KR440_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBH0700669.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
     gene            complement(813..989)
                     /locus_tag="KR440_003718"
     CDS             complement(813..989)
                     /locus_tag="KR440_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700670.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(1043..1132)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          486 a          181 c          129 g          500 t
ORIGIN      
        1 taactaattt tatatttatt atatttcagt gaaaaaatag aacattgaaa catatagata
       61 tgttttaatt gctttttatt aacaaattac taaaagtgtt atatcagaat tgaatacaaa
      121 agaaagagta tgtatttatg agatagttga aaattttgat gtgccacaac taaaattatt
      181 atattactat atattctaat taaaagagta cttgtaggtt taagtacata ctttataaat
      241 atatttttgt cagatttatt ttataaaaag tatttagact aagtattatt tttaagtatt
      301 ttcttgttga agttattttt tcctttttta caaaatgcgt aaataaaaaa aagactctta
      361 aaagagtctt tccgaaaaat ctatatatta taatatagtt atattttctg cttgaggacc
      421 tctagcgcct ttaactatat caaagcttac tttttgacct tcttctaatg atttaaatcc
      481 tgaagtttgt atagctgaga aatgagcaaa tacgtcatct ccactttcca ttgatataaa
      541 tccaaatcct ttttcattat taaaccattt tactattcca ttattcatta aaaatactcc
      601 tattaaccta tatcaagata tacttgattt acttgtgtat taccacaaat actattatat
      661 catagattaa cataaatgtc tatttaatta taatttttag ttaacatttt tatttattct
      721 taatatgaaa ataaatttaa acattttata ttaccaatat attaatgtaa atgtagataa
      781 aactaaaaag ggttttctag aagaaaatct gtctaattat acttacttac caataccatc
      841 acttgatgca agcaagattc cacaactttt tctccgtact ctaatataaa atcatctaac
      901 tgtgtatcta taaaatcaca ataatcatat gaactaccaa tataagcagt tagcatatgt
      961 gtacatttat ccattttatc tctaaacata atgtatttat ccccctaaaa aactttcatg
     1021 aaaaacaaaa agaaaatatc tttttggcaa ctggctgaca taacttattt tataagcgtt
     1081 agtccctata gctttgcgtc actaaatttc tctagttttg ccgatttagt tttattctac
     1141 agattaataa tacaatagaa ttagctatta ttcaacatat ctagctgaaa aattaataaa
     1201 tttatatatg aacgttaaat tatttgaaaa aataaaaagc ccctctgtct gaactatacc
     1261 cctcttagga gacagttgaa atatgtaaaa ctgtct
//
LOCUS       DAEPOZ010000412         1286 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.416, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000412 DAEPOZ010000000
VERSION     DAEPOZ010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.416"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            306..1211
                     /locus_tag="KR440_003719"
     CDS             306..1211
                     /locus_tag="KR440_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P63C domain-containing protein"
                     /protein_id="HBH0700671.1"
                     /translation="MESKEKSILRATHFGIIKIGEKDLNCAVLENGSRIINKSTVFKA
                     FGRTKRGRKKDEIRVPSKPNLPSFIDAKNLSDYISDELEDMLINTIKYKTKNGRIVEG
                     YPAEIIPLICDVYLQARLDNKLAVQQEGLAIASEILVRSLSKIGIVALVDEATGYQYD
                     RDRDELQKILSMYISKEFLPWTKRFPDEFYRQMFRLKNWTYPRPSSKRPGIVGTYTNK
                     YVYDLLPPGVKEELQRVNPTVKPGQRKHKHHQFLTEDIGNDHLKNHLLKVITLMQASK
                     DWKDFNVLFNRAFNLPVQLEIEYDE"
BASE COUNT          538 a          155 c          191 g          402 t
ORIGIN      
        1 tgcaatattt gcaatagtaa gttttcaatt acaactaata ccattatgca taaaactaaa
       61 ctagactaca gaaaatggct acttgctatt aatttattta tatttgatga aaatattagc
      121 tatagaaaat tatcaaaaaa tatcgttgtt aataaaaata ctgcgtacaa gatgataaaa
      181 aaactcaatt atctatatac atattttaaa attgatatta ttagtcttat aaatactaat
      241 ttatctaatg aagagatact aagtaatatc ttacttatta aaataaaata aaggatggtg
      301 ttaaattgga atcaaaagaa aaaagtatat tgagagcaac acattttggt attataaaaa
      361 taggtgaaaa agatttaaat tgtgctgtac tagaaaatgg ttcaagaatc ataaataaaa
      421 gtacagtatt caaagctttt ggaagaacaa agcgtggtag aaaaaaagat gaaattcgtg
      481 taccttctaa gcctaatcta ccaagtttta ttgatgcaaa gaatctatct gattatattt
      541 cagatgaatt agaagatatg cttataaata caataaaata taagactaaa aacggaagaa
      601 ttgtggaagg ttatccagct gaaataatac ctcttatatg tgatgtatat ttacaagcaa
      661 ggcttgataa taaattagct gttcagcaag aaggactagc tatagcaagc gaaattttag
      721 taagaagttt atccaaaata gggattgtag cattagttga tgaagctaca ggatatcaat
      781 atgatagaga tcgtgatgag ctgcaaaaaa tattgtctat gtatatttct aaagagtttt
      841 taccttggac aaaaagattt cctgatgaat tttatagaca aatgttcaga ttaaaaaact
      901 ggacataccc tagaccaagt tctaagagac ctggaatagt tggaacatac acaaataaat
      961 atgtttatga tttattacct cctggagtta aagaagaact tcaaagagtt aaccccacag
     1021 taaaacctgg gcaaagaaaa cataaacatc atcaattttt aacagaagat ataggtaatg
     1081 atcatttaaa aaatcattta ttaaaggtta taaccttaat gcaagcatca aaagactgga
     1141 aagatttcaa tgtgcttttt aatagagctt tcaatctacc tgtacagctc gaaatcgaat
     1201 atgatgaata atagactata gcctttaaca acgctttaaa tacttttaat ttatattata
     1261 aaaatagaat taacttatat cataaa
//
LOCUS       DAEPOZ010000413         1285 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.417, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000413 DAEPOZ010000000
VERSION     DAEPOZ010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.417"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(360..749)
                     /locus_tag="KR440_003720"
     CDS             complement(360..749)
                     /locus_tag="KR440_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH0700672.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
BASE COUNT          433 a          210 c           90 g          552 t
ORIGIN      
        1 tttaaatatc ttcattaatt cctatttatt ttatcatctt aaatacgaaa acactgtgtc
       61 atatttgcaa aatatatatt gtcatctata ttaaagcttc acaatttata tttaaaaatt
      121 ctctttaatc acactacctc tatttaaaaa gctaccttaa ttatacttat aagtaaaggt
      181 agctttttaa atttaattaa atagaatatc cactatcaat ttatatttaa tttatttaga
      241 ttcacattat agtatcataa tccacttata atcatttgtc atattactta agtagactaa
      301 tatattacat aaatacactt acatacactt tatattaatg ctataagaaa tacgtttgct
      361 tatacgtctt tctcctcttc ttttagattt tttatccact cttcaataag taagatatca
      421 tctttgctca aattaatatt ttcatgtaat gacaacaata aactttttat agaagaatca
      481 tgaatatttc taaaaaaatc tcttgtctca actcctatgt attccttttc tttaattaaa
      541 acttcataat gtgtatattt atctatcttt tgagaattta gaaatctttt tactactaat
      601 ctagataaaa gagtcaaagt tgtcgtttgt ttccattgat atttttgctc cattcctaaa
      661 acaatatctc tagatgttac tttggaatct gatttccata taaatttcat tatctttaat
      721 tctgattgtg gcaacttttt tatagtcatg ataaaacctt gctactcaga atatagacaa
      781 gcaatatcca taataaaatt tttcgattaa atacatctta tacggattta tataagctca
      841 gacttataag atatttttca atcttaatcc taaatttagc tatttgtaca cggtaaatat
      901 atataaataa tgtcaaaatt ctcttgtatt atattgctta attttatgag atattttgta
      961 ctcagctatc tattttggat acttgtccag tagctctcct ttcttttaaa ctctttgatt
     1021 ttaaatattt gtatttcagt aaatcctact tcctaaattt tttatttact acaacttatt
     1081 actgaacttc ctacttccta aacttttcat ttactacaac ttattactga acttctcact
     1141 tactaaattt cttacttact ataaattata actaaacttc tcacttacta aatttcttac
     1201 ttactataaa ttactactaa acttcctact ttctaaattt cttacttgct ataaattatt
     1261 actaaacttc ctactttcta aattt
//
LOCUS       DAEPOZ010000414         1283 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.418, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000414 DAEPOZ010000000
VERSION     DAEPOZ010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.418"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(126..1046)
                     /locus_tag="KR440_003721"
                     /pseudo
     CDS             complement(126..1046)
                     /locus_tag="KR440_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428810.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
BASE COUNT          437 a          170 c          161 g          515 t
ORIGIN      
        1 atattattat ataatataat gataaatata tattaacaaa taataaaaaa attaatttga
       61 ttgtatataa attagtttgt tattaaatta gtattaaaaa gttcaaaaat ttacttagaa
      121 agaatctata attcgatttc tatgtttgtt gagaatgcaa ttacagcttt tccaattatt
      181 tttacttcaa ctggcttatt atctgaaata attactttta ctaacatctt gcctttgcgt
      241 tttattgctt ctccttgaac tgcttcaaat ataaactctg tgttgggtat aggtattact
      301 ttataatgta caaggtacgc acctaaaggt ccattagcat ttcctgtaac agggtcttct
      361 gaaataccaa ttgcaggagc aaacattctt ccatgaactt gtagagtttc tttattatgt
      421 aatgtaaaaa tataatatcc attacatttt atttcttgac ttattttcga taattcatat
      481 aaatttggag ataaactatt taatgttgat aattccttta ttccaatcat aacttttgaa
      541 tgtcctgtag atgatatggc aataggacaa tctgtacgaa tatcttcatt taaaatacca
      601 aatgcattaa gtaatctttt ttgataggtt tgttgtaatg gtggagatac ttcaattttt
      661 ccttgtgtca taattatttc ataatcatta tcttttttta taatatcaac tggtaatatt
      721 ccagcaccag ttttttgaaa tactgttttg ttattgaatt ggttttctac tgctctaaca
      781 taatgggcag caatagtagc atgaccacat ataggaactt cattagttgg tgtaaaaaat
      841 ctgatatgta catcataatt ttcttgatga tttttagcca atttattgaa aataaaagca
      901 gtttatgaat tatttaattc tcttgttatt ttttgcattt gtgattcaga taagccatca
      961 gcatttgtaa ttacaccagc tggattacct gtgaatcttt cttttgtaaa tgagtcaact
     1021 tgataaagac gaaatttttt attcatatta tataatcctt tctaaacatt agattagttt
     1081 atctacatag aaaacttgag ataaattatt atttattatt atctgtttaa ataaaattaa
     1141 taaaataaca tatgtctata ttaaaattta aaaatataca tctaaaaaag tagtattatg
     1201 taaggctcta tgctacactg aacctcccat gactaaagtc acagggttcc tgcttcatag
     1261 aattttgcat actaaaatat gtc
//
LOCUS       DAEPOZ010000415         1240 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.419, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000415 DAEPOZ010000000
VERSION     DAEPOZ010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.419"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            142..609
                     /locus_tag="KR440_003722"
     CDS             142..609
                     /locus_tag="KR440_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBH0700673.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFSDYFIVILFLIFIINSTKYSPFILENVKRFKVMGCCLLINTIIECVIG
                     YNSNTSLIMIFGDNSGGISPLMVISFIFALMCFVIAETFDKAIKIKEDNDLTI"
     gene            620..835
                     /locus_tag="KR440_003723"
     CDS             620..835
                     /locus_tag="KR440_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBH0700674.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
BASE COUNT          446 a          145 c          149 g          500 t
ORIGIN      
        1 ataaatttac caccttatac attcttattt ttagtgtata ttataatggt tttatatatt
       61 atatcaacaa tttattattg aaattcaata aattatatat tataatcaat aatatacttt
      121 atataaagga gatgatattt tatgattaca aaatttttta gtaatatatt atcagtattt
      181 ctatattttc tattatcact tttaggagtt gctggtataa tattcttatg tatgatacct
      241 atgtttttat atgattttag ttctgacaaa gtaatcgctt taattgcaag aatactatat
      301 ttttctgact actttatagt catcttattc ttgatattta taattaatag tactaagtat
      361 tccccattca tacttgaaaa tgtaaaaagg tttaaagtaa tgggatgttg tttattgata
      421 aacactataa ttgaatgtgt aattggttat aactcaaata ctagtcttat aatgatattt
      481 ggagataata gtggcggtat ctcaccactt atggttattt cttttatatt tgcactaatg
      541 tgctttgtaa ttgcagaaac atttgataaa gctataaaaa taaaagaaga taatgattta
      601 acgatataga ggtgatgtta tggcaatagt agttaaccta gatgtaatga tggcaaaaag
      661 gaaaatatca cttaaagatt tggcagaaaa aatagatatt acaaatgcaa atttatcaat
      721 tttaaaaact ggaaaagcaa aagctattag atttaccact ctaaacgaaa tttgcaaagc
      781 tttagactgt cagccaggtg atatacttga atatgtagaa gatgaaaatg attaaaaaag
      841 agtgtttaga aacactcttt tttattatac tatatgattt ttattctttt actatattat
      901 tgacattaat attaactttg tttaaagtca taacaaaata cgttctaaga aagaaactgt
      961 tcctttttct atttgttatc cattacctat aagaactgta tcacactaaa tcatactttt
     1021 aaattgataa ttttgccaca gattcctata tacattcttt tctacctaat atttatttaa
     1081 actgttagca aaacatggaa ctttttcatt tacttatacc tccatttata ttattgcata
     1141 gtttatagct acgtgttata aattacatta ttgatgattt atctaacatt tgtaatcttt
     1201 tagatactct ttgaagttac aacactgtta tactctaatt
//
LOCUS       DAEPOZ010000416         1231 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.420, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000416 DAEPOZ010000000
VERSION     DAEPOZ010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.420"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            221..514
                     /locus_tag="KR440_003724"
     CDS             221..514
                     /locus_tag="KR440_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBH0700675.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            495..1136
                     /locus_tag="KR440_003725"
     CDS             495..1136
                     /locus_tag="KR440_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBH0700676.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVILQI
                     LMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKQNYSVGIILPWTLDDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISGKNN"
BASE COUNT          465 a          122 c          186 g          458 t
ORIGIN      
        1 tttaacaatt gtggtgcaat acacttttgt taaattaaaa aaattagata aaataagatt
       61 atataaaagc gacttaagta aattgagaat cttaatatga aaattttcaa taaatatttg
      121 gaaataacta tttagaaatt tttctaaata gttattgaaa taaaatggat gttatgttat
      181 attctattta gatgaaattc taaatagcga ggtgatggtg ttggggtttc cagaaacatt
      241 caaagctctt tcagacccta taagaagaga aatattggtt atgttgaggg agaataaaat
      301 gtcagcagga gaaattagtg aaaaatttaa tatgacgaat gctacaattt cttatcatct
      361 atctcaacta aaaaaggcag gactcatatt tgaaacaaag tataagaact ttattttcta
      421 cgaaataaat atttcagttt tcgaagaggt tatgctttgg ttttcacaat ttatggagaa
      481 gggagaaaaa gataatgaaa aaagcgataa ataaacaatt tattttatct actttaatat
      541 gttttatacc atttattgta tcaatatatt tttataatag attaccaaat gaagttgcaa
      601 ttcattttga taattatgga aatccagata attatgctcc aaaggttata gcagcttttg
      661 gtgtaccatt gttaatgcta tgtatccatt tgtatacatg gtttagactt gagaatgagc
      721 ctaaaaaagc taatttttca aatgttatac aaaatttatc aaaatgggga gttgctatac
      781 tttcagtaat tctacaaata ctgatggttt tatattcaac aggagttagt ttggagtcta
      841 atttttatac aggattaatt ttaggaataa tagttatttt atttggaaac tatcttccta
      901 aatgtaaaca aaattactca gtaggaataa ttttgccatg gactcttgat gatgaagata
      961 attggaataa gacacaccat ttagctggat ggatttggct tattggtgga atattattaa
     1021 ttattaatgc atttattaat attccttttt ataatatctt tgtaatcttt gtaattgtaa
     1081 tactaccatt tatatattct tatttgatgt ataaaatttc aggtaaaaat aactaatata
     1141 attaatcact ataataatgt ttggataaga ataatatatt tcagaactga gatagataat
     1201 aaagaaatta ctaagatttc tattttagtt c
//
LOCUS       DAEPOZ010000417         1206 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.421, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000417 DAEPOZ010000000
VERSION     DAEPOZ010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.421"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            23..>1206
                     /locus_tag="KR440_003726"
     CDS             23..>1206
                     /locus_tag="KR440_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBH0700677.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVGPSKIRTFGIPVEKSFLSHRDKD
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNVKSIRKSNSAQNIA
                     NL"
BASE COUNT          461 a          162 c          195 g          388 t
ORIGIN      
        1 tacattttag gaggatttta ttatgagcaa aaaagtatta ataatgtctg cttccactgg
       61 tggtgggcat aatagggctg ctctagctat taaagaagaa cttacttcca aaacacttga
      121 tggagagcca attgaatgtg aaataataga tagtttgaaa ttagttaata atacaatgga
      181 taaaataatt tcaagaggat acgaaaaatc tgctatatat actcccaaag cctatggaag
      241 tgtatataga ttatctgaaa caaatctttt gtcaaaaaat gaatttaaag ataatctact
      301 aataacattc atggctaaaa aatttaaaaa gcttattaga agcgaaaaac cagacttgat
      361 tataggaaca catccttttc caatgattgc tctaagcaca ttgaaaaaaa actttaattt
      421 acataataat gaatcaaatg cttacacaga gcatttttat aaacattata caaataccat
      481 taatgttcct ccgcttattt ctgtattaac cgattatact actcattcta catggataca
      541 aaatgaaatt gattactata ttgttggtca tgaatatgta aaagaattat tggtgtttga
      601 tggagttgga ccgagtaaaa ttagaacatt tggaatccct gttgaaaaat cttttctctc
      661 tcacagagat aaagatattg ttctttctga gttgaattta tctcctgata aattaactgt
      721 tttacttatg gggggtagct ttggggctgg aaatataaaa gagacacttg atgaattact
      781 ggatactgat agagattttc aaatacttgt aattactggt aagaatgaaa gcctaaaaga
      841 aaaaatcgaa aaaaaactta tgtctcgtta tcatgataaa aatgtatgtg ttcttggata
      901 cactgataag atgaatgata ttttagcttc tatagatgta ttagtaagca aacctggtgg
      961 acttactaca actgaggctc ttttaaaaga tgtacctatg atagttcctt attatatacc
     1021 aggtcaggaa gaagagaatc ttgatttctt atctaattgt ggtgcagcac ttagaactac
     1081 aaaaaaatat aacttgtcaa tattactaaa agttctaata gatgacccta gcagattaga
     1141 aatgctaaaa agaaatgtta aatcaataag aaagtcaaat tcagcccaaa atatagctaa
     1201 tttaat
//
LOCUS       DAEPOZ010000418         1198 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.422, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000418 DAEPOZ010000000
VERSION     DAEPOZ010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.422"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..891)
                     /locus_tag="KR440_003727"
     CDS             complement(<1..891)
                     /locus_tag="KR440_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0700678.1"
                     /translation="MKYLIEIAKKERLLVFIYLALGIGIELLNSFSANYYQSLIDRFN
                     NSTINFHIIFIYGTVLVTLCLLRYIDEYPGRRLEHSFFLDLKLKALEKMSKIDYLEYQ
                     MLGTGKLIQKIENGANAGRNIFFNFLLAVVRKIIPSILFSMVFIYRIDKKVMFTILIG
                     YFIVFIITNLLIKALYQIKEHILINEEKMNHFLVRGFVEMVVFRVNKRFEYEICQSKF
                     AKKEIIDSRVKMTLIHEAFFTIFSLIVTFIKMLIIVYSWKTKTISIGSIIALITLLDN
                     AYTPIAIFNVLYIQFKLDKAA"
BASE COUNT          444 a          171 c          100 g          483 t
ORIGIN      
        1 tgctgcttta tctagtttaa attgaatata caatacatta aaaatagcta taggagtata
       61 tgcattatct agtaaagtta tcaatgcaat tatagaacct atacttattg ttttagtttt
      121 ccaactataa actattatta acatttttat gaacgtcact attaatgaaa aaatcgtgaa
      181 gaaagcttca tgaatcagtg tcattttaac cctagaatca ataatttctt ttttcgcaaa
      241 ttttgactga caaatttcat actcaaatct tttgtttacc cgaaatacaa ccatttctac
      301 aaatccacga actaaaaaat gattcatctt ctcttcatta attaatatat gctctttgat
      361 ttgatataga gcttttatta gtaaattagt aataataaaa actataaaat atccaattaa
      421 tatggtaaac ataacttttt tatcaattct ataaataaaa accatactaa ataaaattga
      481 tggaattatt tttctaacaa cggctaataa aaaattaaaa aatatatttc tgccagcatt
      541 agctccattt tcaatttttt gaattaattt tccagtgcct aacatctgat attctaagta
      601 atctatttta ctcatttttt ctaaagcctt taatttcaaa tctaaaaaaa aactatgttc
      661 tagtcttcta cctggatatt catcaatata tcttaaaaga cataaagtaa ctaatacagt
      721 tccatagata aaaattatgt gaaaatttat agtactgtta ttaaatctat ctattaggct
      781 ctgataataa ttagcactaa aactatttaa aagttctatt cctataccta gtgctaaata
      841 tataaaaact aataacctct ctttttttgc tatttctatt aaatatttca taactgtaca
      901 cctatcctta tttattttac tttattaact taaaatagtt ttatagataa aatgtttctt
      961 tatttgttta aatttagtgt gattattaat catttcaagt taacaaaacc taaagttttt
     1021 agcagtacag ttctatttgt tagcaaatta ttattttact cagtacatta tgctaacata
     1081 taatctaact gtactgagta aatatctcgt ttcatcatat aaaatctcct ttttatagtt
     1141 taatatatat agttaaattt actatataat ctgaatattt atttttctat caccttta
//
LOCUS       DAEPOZ010000419         1186 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.423, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000419 DAEPOZ010000000
VERSION     DAEPOZ010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.423"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            256..1152
                     /gene="ylqF"
                     /locus_tag="KR440_003728"
     CDS             256..1152
                     /gene="ylqF"
                     /locus_tag="KR440_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBH0700679.1"
                     /translation="MSNEYEEYLMDDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINNIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIAATV
                     LNEFRDGKIGNITLEVPENVKR"
BASE COUNT          515 a          111 c          224 g          336 t
ORIGIN      
        1 cagcaaataa ttatactaga aataaataga aatatttgtt gaaaatatac attatttatc
       61 tatgaattta tagcttttaa aatgaaaaat aaaattggta aatattggat tatattttgt
      121 aacgatatta aaaataagag aacagtatta tgtaaagtat gacgttaaaa agatgacaat
      181 attatagttt gtgaagatag attttttaag tggaattaga atataataat ataatattaa
      241 taaggagtga tacatgtgtc aaatgaatac gaagagtatc ttatggatga caatctacat
      301 ataaactggt atccaggtca tatgaaaaaa actaaggagt tagtaaaaaa taacttaaaa
      361 ttgattgatg tagtaattga acttttggat gctagaatcc cgtttagtag taaaaatcca
      421 gacatagaca ggcttgtagg agataagcca agagttgtcg tacttaataa gagtgatatg
      481 gcagataggg ataaattgaa tcaatggatt gaatactata agaaaattaa tatcaaagct
      541 ataccagtag atacaataaa aggtgttggt ataaataata taatcgaaga atgtaaaaat
      601 gtaactagag aaaaaatgag ttctttaaaa gacaaaggga gaaaagaaag agctatacga
      661 ataatgatag ttggagttcc aaacgttggt aaatcgtctc ttataaacaa attaacaggt
      721 agaaaaagca ctcaaactgg agataagcca ggagtaacga aagggaaaca atgggttaga
      781 ttaaagggaa atcttgaact tttagataca cctggaatac tttggcctaa gtttgaagac
      841 caagaagttg cattaaattt agcatttagt agagctataa aggatgagat tttagatact
      901 gagactttgg ctttaagact tatagaaaaa cttatgaaaa tagaacctga gaagttgaaa
      961 gccagatata agttagattg tctaggagaa actccaattg aaactatgga tatgattggc
     1021 cataaaagag ggtttataac aggtaaaaaa gaactagatt atacacgcat agccgcaact
     1081 gtattaaatg aatttagaga tggaaaaata ggaaatataa cattggaagt tccagaaaac
     1141 gttaaaagat agattttaaa acaaaatcaa agatttaatg ttaaaa
//
LOCUS       DAEPOZ010000420         1174 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.424, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000420 DAEPOZ010000000
VERSION     DAEPOZ010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.424"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..915
                     /locus_tag="KR440_003729"
     CDS             <1..915
                     /locus_tag="KR440_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBH0700680.1"
                     /translation="FNYYIWLVIVFRMLLFLFNYTVMYEVKEPKENTVGNNITQIDVS
                     IDNDFMLYLVYLWLFVTIAIAVYTFMEYTKFKNLVVDVSYDIEDHDVNCIYKNLLKEL
                     DIKKKIELRGSDELISPAGMGLFKSYIFLPDYTYSKDELTWILKHELMHFKNKDILIK
                     FLVLSVKIMYWFNPLVYIMSNKVNLDCELCCDESVLTDCSLKDKKAYALALIKSIKLS
                     KNYNSGILTTEFNKTNLEKRLDSIIKKKGKSGILMLLILFTLFSVTYFNFESISFDNS
                     NYLRNIGILEKTGADLKEEDDISMRVVN"
BASE COUNT          466 a           91 c          187 g          430 t
ORIGIN      
        1 tttaattatt atatttggtt ggtaatagtc tttagaatgt tactttttct atttaattat
       61 acagtaatgt atgaagtaaa agagcccaag gaaaatacag taggaaataa tattacacaa
      121 attgatgttt ctatagataa tgattttatg ctgtatttag tttatttatg gttatttgta
      181 actatagcca ttgcagttta tacatttatg gaatacacaa aatttaaaaa tctcgttgtt
      241 gatgtatctt atgatattga agatcatgat gtaaattgta tctataaaaa tttgttaaaa
      301 gaattagaca taaaaaagaa aattgagtta agaggttctg atgaattgat aagtccagca
      361 ggtatgggat tatttaaatc ttacatattc ttaccagact atacttatag taaagatgag
      421 ttaacttgga ttttgaaaca tgaattaatg cattttaaaa acaaagatat tttaataaag
      481 tttttagttt tgtctgttaa aataatgtat tggtttaatc cacttgttta tataatgagc
      541 aataaagtaa atctagattg tgaattatgt tgtgatgaaa gtgttttgac tgattgctct
      601 ttaaaagata agaaagcata tgcgttagca cttataaagt caattaaact tagtaagaat
      661 tataatagtg gtattttaac aactgaattt aataagacaa atttggaaaa gagacttgat
      721 agtattatta agaaaaaagg gaaaagtgga atattgatgt tattgatttt gtttacatta
      781 ttttcagtta catattttaa ttttgaaagt attagttttg ataattcaaa ttatttaaga
      841 aatataggga ttttagaaaa aacaggcgca gatcttaagg aagaagatga tattagcatg
      901 agagttgtaa attaatagat ttttatagta ttataattta ttaacaaatt aggagaagtt
      961 ttgaaattaa aaatctaaaa aaaatcttag tagtcacagg gagggtgact ctaatatcta
     1021 ttagtttagt atttgcaaat actaataatg atgtaaatgt atgaaaaagt aatgaagagc
     1081 aaacaacaaa ttcatttaca ataactggtt acgaatttat ttatgaaaat gtaccacaac
     1141 ctataaaatc tgattatgaa aaaaatgtaa agaa
//
LOCUS       DAEPOZ010000421         1167 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.425, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000421 DAEPOZ010000000
VERSION     DAEPOZ010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.425"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(256..1125)
                     /locus_tag="KR440_003730"
                     /pseudo
     CDS             complement(256..1125)
                     /locus_tag="KR440_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
BASE COUNT          447 a          179 c          107 g          434 t
ORIGIN      
        1 tctaaaataa atatttataa attctaatca atcctaaata ataatctaaa atacatcaaa
       61 aaataaacta aaaaatatcg aaaaataatt taaaagacat caaaactaaa tcttctaatc
      121 aacaattaaa agtctatttt cttagtttaa aaaataaaga tagatttttt caaaacactg
      181 gataaatagg taaaaaaata aacagttagt aaaataaatt tttactaact gtttattttt
      241 attgtttaat atgtcctata aatcctctaa gtaatatttt ccttgcaaaa actctgattc
      301 taaacctaga tttttaaaat aatcaaccat gcccatacac gcaaattttt ctgtagaata
      361 atgagtagca cctataacat taattaaatt atcttttgct atttgatgga actctaatac
      421 tggttcaaaa tgttttaaag gcttagtaaa cccagtaata tatagattta taccaagttc
      481 agcaacttct cttgctacaa atggataact tcctccccct gctgcaatag caactaaacc
      541 atcttttaat acagaatctc cataatcata caatttcaca tcatgaccaa cagatttttt
      601 aattaattcc ttaatctctt ctattgattt tagtgatgtt ttacatataa ctcctacctc
      661 aattttatcg tcatatttac aaaatggttt gactatttct aaacccatag cttttgcaaa
      721 actaacagaa gttgaatagt cactatagtt gtctagtgga gaatgtaata cataaaaaga
      781 aattctacga tttttcatct cttccatata agatagtggt atgtcgtgaa atggaaaacc
      841 ttcatctgat gctatgtatc ccattgcgtg atgtgaaaaa attaaaatat ctttttcatc
      901 tttatctata acttgcttaa ttatttcttt atcaggaaaa gtagttgtgt atactttatt
      961 aatattttga gcattatcta atactaatca tatgtacttt tttctaaatt ctggagtgat
     1021 aaaatcattt tcaaaattca taaaactcca atcatcatta atatttttaa tattaaattc
     1081 taaatctaat tttttataaa gttcaattgc cttaataata accactcctt tataataata
     1141 ttgaattact acatattaat atagaaa
//
LOCUS       DAEPOZ010000422         1146 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.427, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000422 DAEPOZ010000000
VERSION     DAEPOZ010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.427"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..>1146
                     /locus_tag="KR440_003731"
     rRNA            <1..>1146
                     /locus_tag="KR440_003731"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          293 a          253 c          352 g          248 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtactag gtgtcggggg ttaccccctt
      601 cggtgccgca gctaacgcat taagtactcc gcctgggaag tacgctcgca agagtgaaac
      661 tcaaaggaat tgacggggac ccgcacaagt agcggagcat gtggtttaat tcgaagcaac
      721 gcgaagaacc ttacctaagc ttgacatccc aatgacatct ccttaatcgg agagttccct
      781 tcggggacat tggtgacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt gagatgttgg
      841 gttaagtccc gcaacgagcg caacccttgt ctttagttgc catcattaag ttgggcactc
      901 tagagagact gccagggata acctggagga aggtggggat gacgtcaaat catcatgccc
      961 cttatgctta gggctacaca cgtgctacaa tgggtagtac agagggttgc caagccgtaa
     1021 ggtggagcta atcccttaaa gctactctca gttcggattg taggctgaaa ctcgcctaca
     1081 tgaagctgga gttactagta atcgcagatc agaatgctgc ggtgaatgcg ttcccgggtc
     1141 ttgtac
//
LOCUS       DAEPOZ010000423         1134 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.428, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000423 DAEPOZ010000000
VERSION     DAEPOZ010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.428"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            119..898
                     /gene="hisJ"
                     /locus_tag="KR440_003732"
     CDS             119..898
                     /gene="hisJ"
                     /locus_tag="KR440_003732"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HBH0700681.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGKELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNTILEDLIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLELVKKYDIPLVLGSDSHCAEEINKYDII"
BASE COUNT          479 a           90 c          187 g          378 t
ORIGIN      
        1 taaaattact ctaatattag atataataaa aattagcatg attaaaaata ttattttgaa
       61 gttaaaaaat ttatgcattt atattagatt ttttctttaa aaaaggagtg aattatttat
      121 gaaagatggt catatacatt caccttattg tcctcatgga agtaaggatg attttgaaaa
      181 atatgtacaa agagcaatgg atgttggaat aacagaaatg acatttacag aacattttcc
      241 gtatgcaaat ggatttagag accctgctcc tgaaaatgat agttgtatga gtattgaaga
      301 tttaccaaag tattttaatg atgtcaagaa gttgaaagaa aagtatgagg gtaaaatcaa
      361 gataaatgtt gggtcggaag tggattttat agaaggatac gaagaaggga taaaggagaa
      421 tttaaataaa tatggaaagg aattagaaga ctctctttta tcagtacata taattaaaat
      481 tgatgatgat tattattgtg tagattacag tgtagaggag tttcaaaatt taatcgacaa
      541 attaggaagt attgaagctg tttataataa gtattatgaa acattgataa aagctgtaaa
      601 ttcagattta ggtatacata aaccaaaaag aataggtcat ttaaatttag ttagaaagtt
      661 taatcaggta tttccatacg attataaagg aaatacaatt cttgaagact taataaagtt
      721 aattaaagaa aaaggttatg aacttgatta taatgtttct ggaaatagaa aagagtattg
      781 caaagagcct tatatagatg gatacttatt agagttggta aaaaaatacg atattccatt
      841 agttttaggt tctgattctc actgtgcaga ggaaataaat aaatacgata ttatttagat
      901 atattcacac aaaagactgt agataagttt gtataaaata aatttatgta cagtcttttg
      961 taaatttata tgattgaaaa tagtaataga acttaaataa aataacaata agttctaagt
     1021 aaaataacaa tagaatctaa gtaaaataat aataaaattc aatagaataa taggacttag
     1081 aattaatcta tcttatttat ataaacattc ttatataaga ttgtagataa gatt
//
LOCUS       DAEPOZ010000424         1124 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.429, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000424 DAEPOZ010000000
VERSION     DAEPOZ010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.429"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            151..405
                     /locus_tag="KR440_003733"
     CDS             151..405
                     /locus_tag="KR440_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700682.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            450..773
                     /locus_tag="KR440_003734"
     CDS             450..773
                     /locus_tag="KR440_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700683.1"
                     /translation="MATEIFTVKITPLEAQKMIKENIDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTEKTVIKATSTGSAIGVLKIDWGAGGNLLKRVKNIL
                     AEYII"
BASE COUNT          471 a           85 c          170 g          398 t
ORIGIN      
        1 tgtatttttt gtttaaaaaa tttaaatata taaaactata aaggttacaa ttttggaaat
       61 agatgtaaat atatgttcta ttgtataata agagtaatat aaaatctagt aaaaataatc
      121 aaataaattt aacttatgag agggataact atggaaaata aggattttaa attaacaata
      181 tgtatatttg cttttgtaat tggagtagta gtattattct gctgtatagg tttgggtgaa
      241 tttagtgcag agcatattat tcaacaaaat ggtggaatca tggatactga ccaatataat
      301 atttatctag accaatctat ttctcaatat agaaattttg gttctatatt agctctgtta
      361 ggaggaatag ggatattgtt tgataaatat atcagaaaat actagagatt atgttttgtc
      421 taaaataaat tcttatgagg agataaaaaa tggcaacaga aatatttact gtaaaaatta
      481 cacccttaga agcacaaaaa atgataaaag aaaatattga tgcagattta gtttttagtg
      541 attcttataa tttaggagat gataaattta tactaattac aacttttgag aagtattacg
      601 caagaaataa tagtgatgca ggattaatcg tagtttatga aaataccaca gaaaaaacag
      661 ttattaaagc tacttcaaca ggcagtgcaa ttggagtttt aaaaattgat tggggtgcag
      721 gtggaaattt acttaaaaga gtaaaaaaca tacttgctga atatattatt taattatttt
      781 atctaactga taatatgtca agaaaatctc gtaaaagagg ttaagaatat ttttataata
      841 tatagataaa taaggatttg aaggtaaagt ttttaatgta taaagaaaaa taatataata
      901 aaacgaattg gctttataat tctttataag gcaaagagta catttcttta tataattgtt
      961 ggatattaaa taatatgtaa atacaccatt tacatataaa agtttaatta atatgttaaa
     1021 agaaaatgaa atggaaatta ctaaaaaata cttttaaatt ggataatgat tgatattacg
     1081 tttatacaaa aatcaagtag aattttatat attttaaata tgat
//
LOCUS       DAEPOZ010000425         1093 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.430, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000425 DAEPOZ010000000
VERSION     DAEPOZ010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.430"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..922
                     /locus_tag="KR440_003735"
     CDS             <1..922
                     /locus_tag="KR440_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0700684.1"
                     /translation="EAIAIVILFTLVNTVFHFYIYNFLSVKFNYINNISKNIKNIENG
                     NLKYKLEVIGDDEISTLAKSINNITGGLETAVDANIKNERMKAELITNVSHDLKTPLT
                     SILSYVDMLKNNDFDRETINDYINILDKKSQRLKLLIDDIFEASKLSSGDVEFNITKT
                     DIRELLIQSIVEFEDKIQNSSLDFIIETPEYAVFTMIDGKKTWRVFENLICNILKYSM
                     PNTRVYIDMFIKEENIILTFKNISNDKLNLKPEELIERFRRGDISRKTDGSGLGLSIA
                     QNIIELENAHMEIIIDADLFKVMLTFRRVN"
BASE COUNT          451 a          111 c          154 g          377 t
ORIGIN      
        1 tgaggcaata gctatagtaa ttttatttac acttgtaaac acagtttttc atttttatat
       61 atacaacttt ttatctgtta agtttaatta cataaataac atatcaaaaa atataaaaaa
      121 tatagaaaat ggcaatttaa agtataaatt agaagttata ggagatgatg aaatatctac
      181 tttagccaaa agtataaata atataacagg tggtcttgaa actgctgtag atgctaatat
      241 aaaaaatgaa aggatgaagg cagagcttat cacaaacgta tctcatgact taaaaactcc
      301 acttacatct atattaagct atgttgatat gttaaaaaat aatgactttg acagagagac
      361 tataaatgac tatataaata ttttagataa aaaatcacaa agattaaaat tattaataga
      421 tgacatattt gaagcatcta agctaagtag tggagatgta gaatttaata taacaaaaac
      481 cgatataaga gaacttttaa ttcaaagtat tgtagagttt gaagacaaaa ttcaaaatag
      541 cagtttagat tttataatag aaacacctga atatgctgtt tttaccatga tagatggtaa
      601 aaaaacttgg agagtttttg agaatctaat ttgcaatata ctaaaatact ctatgcctaa
      661 cactagggtc tatatagata tgtttataaa ggaagaaaat ataatattaa cgtttaaaaa
      721 tatttcaaat gataaactaa atctaaagcc agaagagtta atagaaagat ttagaagggg
      781 tgatatatct agaaaaacag atggctctgg tcttggttta tcaattgctc aaaatataat
      841 tgaattagaa aatgcacaca tggaaataat tatagatgct gatttattta aggtaatgct
      901 tacttttaga agagttaatt gatatattaa tataaaaata tattttaagt tatttaaaca
      961 agaaaaaaac ctccttagag gtctttttct aaatgttggg gtttatattt acatattttt
     1021 tgattatacc tttttggtgt gatattaata cattttaaat tatatttctc tataattcat
     1081 cacatatttc atc
//
LOCUS       DAEPOZ010000426         1079 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.431, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000426 DAEPOZ010000000
VERSION     DAEPOZ010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.431"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            203..760
                     /locus_tag="KR440_003736"
     CDS             203..760
                     /locus_tag="KR440_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBH0700685.1"
                     /translation="MRTLKYAILGLLNRNPMTGYDIVKEFNFQLAEFWNAKHSQIYPE
                     LKKLVNEKLIVYDIKISGDVLEKKLYTITEKGKDEFLKWLEKDEPMERTPKDIFRLRM
                     YFSNNLDIDTRLILLEHQLLQHQERITFLYSQMERYSEVPPLDSNDFGDYIVLDGAII
                     REQGAIKWLTNCINHHKNLDNTTLK"
BASE COUNT          405 a          118 c          144 g          412 t
ORIGIN      
        1 atcacaattt tttctgtatg taaatataaa catgtaaata cttacatgtt tatatttaca
       61 taatttcatt tttatatata gttttattta ttttttgttc ctcataattt aaaagttctt
      121 attaaaacat agctatatta attaattcaa actaaatata tcgtgttata ataagtatgt
      181 aattaaagaa agaggtgtat ttatgcgaac tttaaagtat gcaattttag gattattaaa
      241 tagaaatcct atgactggtt atgatatcgt aaaggagttc aattttcaat tagctgagtt
      301 ttggaatgca aagcatagtc aaatatatcc tgaactaaaa aaattggtaa atgaaaagct
      361 tattgtatat gatattaaaa ttagtggtga tgtattagag aaaaagttat acacaatcac
      421 agaaaaaggt aaagatgagt tcttgaaatg gcttgaaaag gatgagccaa tggaaagaac
      481 ccctaaggat atttttcgtc ttagaatgta tttttctaat aatttagata tagatactag
      541 gcttatttta ctagagcatc aattgcttca acaccaagaa agaattacgt tcttgtacag
      601 tcaaatggag cgttatagtg aagttcctcc tcttgatagt aatgattttg gtgattatat
      661 agttcttgat ggagctatta tcagggaaca aggtgctata aaatggctaa caaattgtat
      721 taaccatcat aaaaatttgg ataatactac tttgaaataa taacttattg attttaatag
      781 tgtatttaat tatagacatt aaatttataa aaaagactgt acacaacttt gtctttgtat
      841 acagtcttct atggttgatt attcaagata gataattata atatttaaaa cagttaatat
      901 aaaagttaaa tattttttat taagctatca attttatctt cttattgccc ttcaaaatca
      961 tctttatatt tacaatcttt cacatgaaaa ttactatctc ttcttatata aaaatccagt
     1021 tgatattttg ttactaaatt taatggtaat caaattcttt accaatgaat ataaaaatt
//
LOCUS       DAEPOZ010000427         1034 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.432, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000427 DAEPOZ010000000
VERSION     DAEPOZ010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.432"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..785
                     /locus_tag="KR440_003737"
     CDS             <1..785
                     /locus_tag="KR440_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBH0700686.1"
                     /translation="ISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNISDIWKLNLI
                     KEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKENIIKRLSSIE
                     SVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRFNILGNNNIG
                     SAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNSNNKMYMEKV
                     FKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITK"
BASE COUNT          440 a           75 c          189 g          330 t
ORIGIN      
        1 agatatctaa aatatatgga ataggtagag attcattaat gtattatgaa gaaataggca
       61 ttctaagacc agttagagat ataaatggat atagaatgta taatatatca gatatatgga
      121 aattaaattt aataaaagaa tttagaagct tgaactttcc aatgaagaaa ataaaagaat
      181 acctagatga cagaagtata gagtctacga aaaatatttt aaatgaagaa ctcgatttaa
      241 ttgataaaaa aatagctgaa tttataagcc ataaggaaaa tattataaaa agattgagtt
      301 cgattgaaag tgtaattcaa aatacaaaaa tagatgagat agaggttgta tatatagaaa
      361 aaagaaaagc tttagaatta aatgctgata taaagaggga tgaagacttc gattttttaa
      421 ttcagaaact tcagaaagaa tatgaagata gatttaatat acttggaaac aacaatattg
      481 gttctgcctt ttctactgaa gcaattaata aaggtatatt taatgagttt aaatctgtat
      541 tttgttttct ggaaagtaat gagaaagtat ataatatagt ttttgatgaa ggatattatg
      601 taactttaaa ttatactggg agtaattcta ataataagat gtatatggag aaggtgttta
      661 aatttataga agaaaataat tataagataa ttgctgaccc tatagaaatc tataagatag
      721 atatacatga gactggaatt gtagaagagt ttgtaacgga aattcaagtt cctataacta
      781 aatagccata tttcaatgga ttagatggta aatagaatga aaaaatgttg atttgccacc
      841 gatttgctac tgtgttataa aatcggtagc aaattatatt ttaaattata agatagcatg
      901 aaaaaataat tgtttagggg gttataaatg tgaaatgttc taattgtgga agtgtaaata
      961 ttgaaaaggg tattttaaca actggtggca tatatgctga aactattgga ttaaaatata
     1021 gaggagatgg aaaa
//
LOCUS       DAEPOZ010000428         1019 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.433, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000428 DAEPOZ010000000
VERSION     DAEPOZ010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.433"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            42..416
                     /locus_tag="KR440_003738"
     CDS             42..416
                     /locus_tag="KR440_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0700687.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            422..>1019
                     /locus_tag="KR440_003739"
     CDS             422..>1019
                     /locus_tag="KR440_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0700688.1"
                     /translation="MDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGKTTT
                     LKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIMSKI
                     YKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATSSLD
                     PIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYI"
BASE COUNT          421 a          111 c          176 g          311 t
ORIGIN      
        1 aatgtgtata tactatatat acaatttaag gaggtgactt attgaatata gttattagta
       61 attcgagtgg gaagccaata tatgaacaga ttactacaca aattaaaagt atgattataa
      121 gcggagagtt accagaaggc tcagctcttc caagtatgag actattggca aaggaactta
      181 gaataagtgt aattactaca aaacgagcat atagcgattt agaacgagac ggatttattg
      241 agacagttac tggtaaaggc agttttgtat ctagtaaaaa catggatttt gtcaaagaag
      301 aacaatttcg tttaattgaa gaatacctac aaaaagcagt tgatacagca aaaagtagtg
      361 atattacata tgacgaatta aaagatattt tatttttatt atataaagga gaatgataag
      421 tatggatgca ataactataa aaaatttaaa taaaacttat aaagatttta gcctacaaga
      481 tatatctttt tcagtaccaa aagggagtgt aatgggattt gtaggtgaaa atggtgcagg
      541 aaaaacaact acattaaaag caattttaaa tttaatatct tatgatagtg gaaatataga
      601 aatatttggt cttgatagta aaaaaaatga aaaagaaatt aaagagcaaa ttggggttgt
      661 atttgaggga agcaactttc atgaaaattt aaacactgac catgtttcaa aaatcatgag
      721 taaaatctat aaaaattgga atgacacatt atttaaagac tacttaaaaa aattgagagt
      781 accagacaat aaattaatta aggagttttc aaaggggaat aagatgaaac ttagtattgc
      841 tgtagcctta tctcataaac caaaattatt aatactagat gaggcaacta gttcacttga
      901 ccctatcgtt cgtgaagaaa tactagatat atttttagat tttatacaag atgaagaaca
      961 ctctattatc ttatcttcac atattacaag tgatttagat aagatagcag actatatta
//
LOCUS       DAEPOZ010000429         1000 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.434, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000429 DAEPOZ010000000
VERSION     DAEPOZ010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.434"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            181..693
                     /locus_tag="KR440_003740"
     CDS             181..693
                     /locus_tag="KR440_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700689.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEDYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKAKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
BASE COUNT          457 a           75 c          185 g          283 t
ORIGIN      
        1 aatatagata aaatcttatt atttaacttc aactattcct taaaaaagca acttttaagg
       61 aatagttgaa acaaaaagtt tggttacata atagtaattt tagttacaag aatgaaactg
      121 taaaacttag gattttcttt atataatttc attgagaaca tttaatttag gaggtaaggt
      181 atgaaagtaa aaaaatttaa aagtggtata gtaatggcat taacagtaat ggcaattgga
      241 gcatcattga caaatgtatc agctatggaa ttaaacaaga gtaaagagtt tgaaactaaa
      301 tacaatgaaa tgaagaggga ttttcaagag gattatggaa aagaagacaa atttaatata
      361 acattggatg atgaatttac aaaagaagaa gtaaatggag ctaaagggat tataaagaca
      421 actaataaaa tgaataatga ggttgtagta ttaaattatt ttgatgaaat gaaatctggg
      481 attgaatttg agttgaaggc aaaagcagaa aaaaatgcaa ttttgagaga tgaatttcaa
      541 caaggttatg gaaaggaaga taaattcaaa gttgtgttag atgatgaatt tacaaaagaa
      601 gaggtaaatg gagctaaggg cattataaag actacagata aagagacagg tgaggttatc
      661 gtatataatt actttgatga gcttgaaaaa taatgcttaa agattacttt gattgaatga
      721 aaaggtaatt gtgttgagaa tattaaatga ctgaataaaa acagttgtat caaaaacaaa
      781 caataataga atgtaagaat gattgtgtcg aaaatattaa atgactgaat aaaaaatagt
      841 tgcgtcaaaa acaaacaata atagaatgta agaatgattg tatcaagaat ataaaatgat
      901 tcaacaaaaa acgattgtgt taaaaacaaa caacaataga atgtaagaat gattgtatca
      961 agaatataaa atgactgaat aaaaacgatt gcgtcaaaaa
//
LOCUS       DAEPOZ010000430          987 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.435, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000430 DAEPOZ010000000
VERSION     DAEPOZ010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.435"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(172..828)
                     /locus_tag="KR440_003741"
     CDS             complement(172..828)
                     /locus_tag="KR440_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700690.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGARILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
BASE COUNT          353 a          188 c           87 g          359 t
ORIGIN      
        1 ttatttaaaa tgctacttct cacttagaat attactcctt attatttaaa acactatcta
       61 tcacttagaa tattccttct tatctaaatt attatttatt ataaatataa ataatattaa
      121 tttaaaaagt tattcataat ttaaataata ctattatctt catcagtgaa cctaaactgc
      181 aactttagac ttattagcta aagccaatat tagacctaaa gctatagatg caaatgcaga
      241 agcaattact ctatactcag cagggaataa gaatacaatt gtatgacatg gtatccagaa
      301 aaatggtata gtttttaaaa cactaaaacc aaggtaagca ttccagtcca caatatttac
      361 cacatcttta agttttacct taatcccctt tccaacctca tattttttat ctataaaaca
      421 gtccataacc ttatggaata aaaatacagt tggtgacatt agtatattca tagttgcact
      481 cttaaataga gcatttgcaa aagctattcc ctcaaatgga agcataccat ccacttgtgc
      541 ttgacctaca cctgatgagt agattgacat tccatatgtg gtaactacac ctattattcc
      601 ccaaactatt gctctataaa atactcctac tggtattttc caatccttat acaaaattct
      661 agcaccaagt aaatctccca ttgtagctaa tacagcaaac tttaaaaaac ccccaatata
      721 tggatgtgca cctgttgctt ccataaatac ttctctacta cttggtattg ctaagacagc
      781 aataatacaa ataagaactc cactccatat aaaatcgcct tttttcatat ttataaatac
      841 tatttaatat ataaatagtt ctcctttcta ttaaagattt atttaattta caatctatct
      901 acaattattt attaatcact tattattaga tattcatttt ccatatttaa atttattcta
      961 tccctttatc tatttatttt cttacta
//
LOCUS       DAEPOZ010000431          985 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.437, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000431 DAEPOZ010000000
VERSION     DAEPOZ010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.437"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            47..781
                     /locus_tag="KR440_003742"
     CDS             47..781
                     /locus_tag="KR440_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis protein TtcA"
                     /protein_id="HBH0700691.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMIYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
BASE COUNT          390 a          106 c          132 g          357 t
ORIGIN      
        1 tcttaaataa atcttaaatt taatattttt aaaggatggt gataaaatgt ctatacaaaa
       61 acttctaagt aaagctcgta aggctataca agattttgat atgatacaag aaaatgataa
      121 aattgcggtt ggactttctg gtggaaaaga tagtttaact ctacttcata tacttaaaag
      181 ttatcaaaga ttttctccac aaaactttga attgatagcc ataacattaa atactggagg
      241 tgttgataat tctccactag ataaattgtg taaagaaata aatgttcctt tttatgaatt
      301 tcaaactgac ataaaagaaa ttgtatttga tataagacaa gaaaaaaatc cttgtgcatt
      361 atgtgctaat ttaagaagag gtgctttgaa cgataatgct aaaaaacttg gatgtaataa
      421 agtagctctt ggtcatcata aagatgatgc aattgagaca tttttaatgt ctatgtttta
      481 tgagggtaga gtaaattgtt tttccccaaa gacataccta gatagacagg atttaacagt
      541 tataagacct atgatttaca tagaagagta tatgactaag aaaatttcaa aagattctaa
      601 ctatcctatt atcacaaatc cctgccctgc aaatggtcat actaggagag agtacataaa
      661 aaatcttatt gctaatttaa ataaagagat gcctgatttt aaaagaaatg tatttggcgc
      721 tttaaataat tctgagaaat tgtttatatg ggataaagag aagataaaaa attttaaata
      781 ataattaaaa tttttaaata aaaaagcaag attgtattaa ttataaataa tctttatata
      841 atctacacaa tcttactttt ttatttatta tatgttatta tctttaatct atattttaat
      901 agttcttaat agtatttatt ttgtctaata cattaattat aacttaaata ttttatatat
      961 taatttaata tattaattca ttcaa
//
LOCUS       DAEPOZ010000432          964 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.438, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000432 DAEPOZ010000000
VERSION     DAEPOZ010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.438"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            30..782
                     /gene="cwlD"
                     /locus_tag="KR440_003743"
     CDS             30..782
                     /gene="cwlD"
                     /locus_tag="KR440_003743"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HBH0700692.1"
                     /translation="MEYYSVFISIHIQGDVVRKYIKHIIFSFAMICLVVVSIFEIKNI
                     SEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGL
                     VILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYG
                     AQTFYPKDKQDSKELSKCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGF
                     LSNEKECKLLTDETYQEKIAWAIYIGIQKYLS"
BASE COUNT          422 a          101 c          153 g          288 t
ORIGIN      
        1 taaaaaaatt taaatgtttg tactaatata tggaatatta tagtgttttt ataagtatac
       61 atatacaggg tgatgttgtg agaaagtaca taaaacatat aatttttagt tttgcaatga
      121 tatgtctagt agtagtatcc atatttgaaa ttaaaaatat ttctgaagat gttatcaagt
      181 atatgccagt aacaaacaaa actataattt tagatgctgg tcatggaggc attgatccag
      241 gtgcattaaa taaggataag agtacatctg aaaaggatat taacctagca ataacactta
      301 agcttagaga gcttatagaa tcaagtggtg gccttgtaat attaactcga gaagatgaca
      361 gtagccttta taaagaagaa aataataaaa ctacaagaca aaaatataat gagaatttga
      421 aaaatagaaa agaaataata tctaattcta atgccaatat gtttgtctct atacatctta
      481 atgcatttga acaatcaaaa tactatggag ctcaaacttt ttatcctaaa gacaaacaag
      541 acagtaaaga attatcgaag tgtattcaag aagaacttaa aagagtagta gataagacaa
      601 acaacagaga agtcaaacca agggatgata tatatctttt gaaagaaaat aatattccat
      661 cagtactgat agaatgtggt tttttatcaa atgaaaaaga gtgtaaactc ttaactgatg
      721 aaacatatca agaaaaaata gcatgggcaa tctacatagg aatacaaaaa tatttaagtt
      781 gaaataagta taaaaatagg tctaaatatg taaatagctt gtattaaagc agttaaaatg
      841 cattaatata ggctattttt attttgacaa aaaaatattt aaaataaaag ttaaaaagtt
      901 gttgacttag aataatatag atgatattat atatgagtgc ccaaaaggag caccaaaata
      961 agac
//
LOCUS       DAEPOZ010000433          951 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.439, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000433 DAEPOZ010000000
VERSION     DAEPOZ010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.439"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..>951
                     /locus_tag="KR440_003744"
     CDS             <1..>951
                     /locus_tag="KR440_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HBH0700693.1"
                     /translation="YTPKIIVDYIVKKTLKNHDIIKNPYPRILDISCGCGNFLLEVYD
                     ILYDLFEENIYELKKKYDENYWTVDNIHRHILNYCIYGADIDEKAISILKDSLTNKKV
                     VNDLDESDIKINLFCCDSLKKKWRYKFDYIVGNPPYIGHKKLEKKYKKFLLEKYSEVY
                     KDKADLYFCFYKKIIDILKQGGVGSVITPRYFLESLSGKDLREYIKSNVNVQEIVDFL
                     GANIFKNIGVSSCILTFDKKKTKETYIDVFKIKNEDICINKFETLEELLKSSKFEHFN
                     INQRLLSDEWILVNKDDETFYNKIQEKCKYSLEDIAISFQ"
BASE COUNT          404 a           78 c          151 g          318 t
ORIGIN      
        1 attatactcc taaaataatt gtagattata ttgtaaaaaa aactttaaaa aatcacgata
       61 taattaaaaa tccatatcca cgaatacttg atatttcctg tgggtgtgga aattttcttt
      121 tagaagtata tgatatttta tatgacttat ttgaggaaaa tatatatgaa ttgaaaaaga
      181 agtatgatga aaattattgg actgttgata acatacaccg tcatatatta aactattgta
      241 tatatggtgc tgatattgat gaaaaagcta taagtatatt aaaagatagc ttaacaaata
      301 aaaaggtagt aaatgattta gatgaatctg atataaagat taatctattt tgttgtgata
      361 gtcttaaaaa gaaatggcga tataagtttg attacatagt gggtaatcca ccctatattg
      421 gtcataaaaa acttgaaaag aaatataaaa aatttctcct agaaaaatat agtgaagttt
      481 ataaggataa agctgactta tatttttgtt tttataaaaa aattatagat attttaaagc
      541 aaggaggggt tggcagtgtt ataactccaa gatatttctt agaaagtcta tcaggtaaag
      601 atttaagaga atatattaaa tcaaatgtca atgttcaaga aatagtggat tttttaggtg
      661 caaatatatt taaaaacata ggggtatcta gttgtattct aacttttgat aagaaaaaaa
      721 caaaagagac atatatagat gtatttaaaa tcaaaaatga ggatatatgt ataaataaat
      781 ttgaaacatt ggaagaactt ctaaagagta gcaaatttga acattttaat ataaatcaga
      841 gattgctatc tgacgaatgg atacttgtaa ataaagatga tgaaactttt tataataaga
      901 tacaagaaaa atgtaaatat agcttggaag atatagcaat aagttttcaa g
//
LOCUS       DAEPOZ010000434          937 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.440, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000434 DAEPOZ010000000
VERSION     DAEPOZ010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.440"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            279..749
                     /locus_tag="KR440_003745"
     CDS             279..749
                     /locus_tag="KR440_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBH0700694.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIEFVKQCENSKE"
BASE COUNT          448 a           64 c          162 g          263 t
ORIGIN      
        1 ggtagaaaca aaagtgttaa tagaagatat tgaaaaaata aaaacaaaag attaataaaa
       61 ggtactgaag agatagagat aaaagtgtta atagaagata ttgaaaaata aaaataaaag
      121 attgatataa tatactgaag aggtagaaac aaaagtgtta atagaagata ctaaaaaagt
      181 aaaaataaag gactaataaa aggtactgaa gagatagaga aaaaaatgtt aatagaagat
      241 actgaaaaaa tagaaataaa aaattaggag gaaaatttat gaaatatcag gtttcattat
      301 tagcagtgaa ggatattgaa gtatcaaaaa aattttacaa agaattattt gagcaagaaa
      361 ttgaattgga cttaggcaaa aatgtaacat ttaaaggagg atttgctata caagaagact
      421 tcgcatggct tacagggatt aatccagata caattataca aaaatcaaat aatatggaac
      481 tttattttga ggttgatgat tttgacgaat ttattgaaaa actcaattat tataaagatg
      541 tagagtttgt ccataagcca ctaaaacatg aatggcaaca aagagtagtg cgtatttatg
      601 acccagataa gcatattatt gagataggag agtctatgga agttatagct agaagatatt
      661 tatctgaggg atactctgta gaagaaactt ctaaaataat tcaacatcca attgaatttg
      721 taaagcagtg tgaaaatagt aaggaatgat tgtttaaatt ataattattt agtagaaatt
      781 aattgtagtg gaactaattc aagataatat ataggactag tatttgacaa atgtcataaa
      841 ttaaattaaa ataaagaaaa tattaataaa gaaaaataag gggaatatta ataaagaaaa
      901 aataaatata atattaaaat atattaataa atttgaa
//
LOCUS       DAEPOZ010000435          920 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.441, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000435 DAEPOZ010000000
VERSION     DAEPOZ010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.441"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            156..>920
                     /locus_tag="KR440_003746"
     CDS             156..>920
                     /locus_tag="KR440_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBH0700695.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCM
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLFLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLL"
BASE COUNT          348 a           84 c          137 g          351 t
ORIGIN      
        1 tagcaaattt agtgatataa ttaaaaaaga aaagaaatat ggacttattt ggaataattt
       61 tgacaatcta tttactacac gtgtagagtc gtatataatt aaattataca taaaatataa
      121 aaaaataatt tagtaatacg ttaggagaaa gtattatgat aaagtcaagc ttattttgga
      181 atattttcat tgcgatatcc tttatgatag aatggtttct ttgtaagaaa atgctagatt
      241 atacaagtga gaaaaggatt tctaacaaaa aaataaactt ttgtatggta ggtattattg
      301 tttttgctga cataatatat ttttttgatg tacctccaaa tataagagta attatatcta
      361 tgataattac tattgcattt tataaaatct catatgatgt gggatttata aagtctgtat
      421 taatatcctt aatttattgg atgttactaa tcgccacaga tgctttgagt atatctttaa
      481 cagtttggct taattctata aatgacatga gtagtatggg gacgggagat atatacagaa
      541 ttgagtctat aatattggga aagatacttt tgattgtgtc tttgttttta tatagagcct
      601 taaagataaa acttaatata gaaataaata aaaggacatt attttatata gttattccta
      661 tagtcgctaa tatagcatct ttttttgtaa tttttgaata catatttaat ccagggtctg
      721 gtagcttaat acataatttc caattcgtta tgatttctgt tgtattgctt atatcaagta
      781 tcactttagt tttaattgct aaaaaattac agcaatataa tactttagtt gtagaaaaca
      841 atatgattaa aaatactttg aagtatgaaa atgagtatta tgaagatata gaaaagaaat
      901 atttaaaaac tagattacta
//
LOCUS       DAEPOZ010000436          915 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.442, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000436 DAEPOZ010000000
VERSION     DAEPOZ010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.442"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..276
                     /locus_tag="KR440_003747"
     CDS             <1..276
                     /locus_tag="KR440_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH0700696.1"
                     /translation="LSRLISDVSWSEFIHQLKYKANWYGRQIVKVGKFFASSQICNKC
                     GYKNEEVKDLNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            435..878
                     /locus_tag="KR440_003748"
     CDS             435..878
                     /locus_tag="KR440_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700697.1"
                     /translation="MKKNMLYLGIVYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GICMIFQYMYWSKPERAVDYEEKIKNQRIEMNDERRVMLRDKAGRITNQIMSYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKKL"
BASE COUNT          327 a           82 c          184 g          322 t
ORIGIN      
        1 ctatctcgct taatttctga tgtatcatgg tcagaattta ttcatcaatt aaaatacaag
       61 gctaattggt atggtagaca aattgtaaaa gtaggcaaat tctttgcaag ttcacaaata
      121 tgcaataaat gtgggtataa aaacgaggaa gttaaggact taaatataag agaatggatt
      181 tgtcctagtt gtaatgaaac tcatgataga gatataaatg caagtataaa tatattaaaa
      241 gaaggattaa gattaataac aattcaaaat aaataacgta tgtaagaacc gtaggaacta
      301 cggggatggc ctgtggagaa ttagtaagac atattttagt atgcaaaatt ctgtgaagca
      361 ggaaccctgt gactttagtc atgggaggtt cagatgagta ttgaagaaat atttatttat
      421 gaggaggagt aaaaatgaaa aagaatatgt tatatcttgg aatagtatat tttatttgtg
      481 gaattttact tgttttattg gctacgttta ctgagttttc atttgaggct tttatttggg
      541 gattatctgg agctgcttta ggtccaggta tatgtatgat ttttcaatat atgtattgga
      601 gtaagcctga aagagcagta gattatgaag aaaagataaa aaaccaaagg atagagatga
      661 atgatgaaag aagagttatg ttacgtgata aagctggacg tatcactaat caaatcatgt
      721 cctatgttct tgtaattctt atatttattg tttctatatt gagtgtattt tctgtaatgg
      781 taataagcaa gtgggtttta gttgttcttg gattgcttat tttatttcaa tttatttgta
      841 gtgttgttgt atacaataaa ttagataaaa agttgtaata aaaagtttaa attcacaata
      901 aaaatattgg atttt
//
LOCUS       DAEPOZ010000437          912 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.443, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000437 DAEPOZ010000000
VERSION     DAEPOZ010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.443"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     repeat_region   474..899
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=540..568
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
BASE COUNT          339 a           79 c          153 g          341 t
ORIGIN      
        1 ttttttagat agtaaacttt aaaatataga tattaattat atgaatataa taaaaaaagt
       61 actaatgagt tacactagta ctttataact acttttacat gttttaactg tataaaacag
      121 tgggtatagt tcagcaggag tgactttagt tttgaactaa aaatcaagtt caaaaagaat
      181 aaacattagt atttgaactt cactctacgt ctaaatagat tgtagttctt cttgttttta
      241 ttataccaca aattggtaca gatattcaaa aataatatat ttatgatata ataaaaatgt
      301 aaatagtttt gcagtgagcg atatttgtta caaagtaggg cttaatgctt gaaatataag
      361 gtgttgaggg tatatgataa actttatcat ttgcactact catggttcac tgcaaattta
      421 agagagttgc acatgtgtaa gtattgaaaa tgcccagttt attttggggt aaagttttat
      481 attaactaag tggtatggaa agaggttttt cagaagtata tgtacgtcta tattgtaaag
      541 ttttatatta actaagtggt atgtaaataa ggaaggtgga agtcaatatg catgggtcaa
      601 atgggtttta tattaactaa gtggtatgta aatacgaaat tagagtttga aatatatgac
      661 tacataatag ttttatatta actaagtggt atgtaaattt gtgtcacatc attgtcactt
      721 ctttagaata tcaaaagttt tatattaact aagtggtatg taaattttta agcaggtgat
      781 taaatgatta aaaaagtaga aaagttttat attaactaag tggtatgtaa ataataactt
      841 gtcaatgtag ttatatgcaa caacttctat gttttatatt aactaagtgg tatgtaaatg
      901 aattatatat ta
//
LOCUS       DAEPOZ010000438          902 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.444, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000438 DAEPOZ010000000
VERSION     DAEPOZ010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.444"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..745
                     /locus_tag="KR440_003749"
     CDS             <1..745
                     /locus_tag="KR440_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0700698.1"
                     /translation="ERFFQNNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQC
                     LSFNQFLASELSDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSN
                     LLDNFFDKNLFIVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDD
                     FLAIPHPMELSSIRTKICISILNEPIYWSEDSTVKLIMMLAINKDDYIKINSIYDILL
                     KIIHDNDIRDSISNCNDFDNFLSIIKSIV"
BASE COUNT          343 a           88 c          117 g          354 t
ORIGIN      
        1 agagagattt tttcaaaata atattaagtg taaaaacgta gtattagtat gtggttcagg
       61 gtatggttca agtaggttat tagaggttca attaaacaaa gtttttcatg ataaaataaa
      121 tatacttcaa tgtctatcat ttaatcaatt tttagctagt gaattgtcag atgtagatat
      181 aataatatcg acaattcctt taaatcatga tagtattcct attgtattag tggatttaaa
      241 actattaaaa aaagatatag agaatatatc taagtcaatc actaataatt cccatattta
      301 tagtaatctt ttggacaatt tctttgataa aaatttattt attgttaatc ctaaaatcaa
      361 agataaagat gagcttataa agttgatgtg caacaaatta actcaaagtg aaattgtatt
      421 tccatcattt gctgaatctg tattttacag agaaagttta tcttctacca atatagatga
      481 ttttttagct atacctcatc ctatggaatt aagctctata agaactaaga tttgtatttc
      541 catattgaat gaaccaatat attggagtga agattctact gttaaattaa tcatgatgtt
      601 agctataaat aaagatgatt atataaaaat aaactccatt tatgatatcc ttttaaagat
      661 aattcatgac aatgatatta gagatagtat atctaattgt aatgattttg ataattttct
      721 ttccataatc aagtctatag tatagatttt tatattacat aagttttaaa aactttaata
      781 aaatattttt tgtttttagc ataaatagta taagtaagtt ttgtataatt tgttattatt
      841 ttgtattgat tataattatt atacaataaa gattctgtct caaaatagag agagaaactt
      901 gt
//
LOCUS       DAEPOZ010000439          901 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.445, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000439 DAEPOZ010000000
VERSION     DAEPOZ010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.445"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..621)
                     /locus_tag="KR440_003750"
     CDS             complement(<1..621)
                     /locus_tag="KR440_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBH0700699.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKFMRENDLENVERVLREHWKTIP"
BASE COUNT          286 a          150 c           92 g          373 t
ORIGIN      
        1 aggtatcgtt ttccaatgtt ctctcaaaac tctttcaaca ttttctaaat cattctccct
       61 cataaatttt actacttcaa tatgttgatt attgctgtat gtcaacattt tatataactc
      121 atcttcatta tcactagtat atgactttga cataaaatta tatcttaaag agttcaatag
      181 atttactaac acctgattat ctgatttagt tatgtatata tcgtgaaatt cattacttaa
      241 ttttaaatag ctattgtaat ttttatactt tatagagacc gccatcattt ctgttaattc
      301 ttccattttt actaggtctg attcagttaa attttgtaat gctgaagaac cggctaacgc
      361 atccaatact ccaattacgg aataggtgtc taatttctcc ttttcctgaa attcttttac
      421 tacaaatccc ttattaggta ttttttctag taaattttca cctgctaacc tagttaatgc
      481 ttctctagct ggtgttctac taactccgat agatttacaa atctcagatt cagtaattct
      541 ctgattagct tcatactctc cattttggat tttctttgat atatagtcat atacttgagt
      601 tgtaagtgga tctaccttca taagacgttc ctccatatta agattataaa agttaaatct
      661 ccctacaact ttggtagaga gttcagtttt atcttactaa tttatataat atcacaatta
      721 tgatattttt tatacttctt tgtaatattt tgaaacacta aaattcttat gataatttat
      781 acaaaaaaag tagaaaaagt tttgataaaa caaaactctt tctacattca ccttattttc
      841 tatttatttt tctaaaaaaa gatatttaat ttactctaaa aattctaaat gatttagagt
      901 c
//
LOCUS       DAEPOZ010000440          840 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.446, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000440 DAEPOZ010000000
VERSION     DAEPOZ010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.446"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            157..657
                     /locus_tag="KR440_003751"
     CDS             157..657
                     /locus_tag="KR440_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBH0700700.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNTINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSSLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
BASE COUNT          359 a           69 c          134 g          278 t
ORIGIN      
        1 taatagagat acaagagata gatatattgg ttttaatttt atagaaatat acaaagatat
       61 tttaagctaa tatatagtaa tttatatagc attataaact agaatcatat aaaataagtt
      121 aactacaatc ataaattaaa aaggagaaat gttaagttga tagaaggaat aaaatttata
      181 aaggcagaag aaaagtatat ttattcgtat tggcaaactt ttgataaaat agcaaaagag
      241 aggaaatatc tagctatgga tgaagccttt ccttttgaag aaacagttga atttataaaa
      301 aatactataa ataaaaactt accacaactg tttataattg atttagaatc agataactgt
      361 attggttggt gtgacgtttt acctaaaact gaaaaagttg gttatttagg catgggtatt
      421 cttaaagagt atagggaaaa aggtatcgga agtagtcttt taaaacaaat tattgattta
      481 tcaaaagaat atgggtatga aaaaattgag cttgatgtat ttaaaagtaa tagtagagct
      541 attcatgttt ataaatcttt agggtttgta gaggtaaata caatatctag tggttttaca
      601 tggaatgata gacctgtaaa agaagaagta attcaaatgg agcttacact tatataaaat
      661 aaagttaaag tctttaaaat tccatataca cttgtataaa ataaagttaa agcccttaaa
      721 attccatata aaagaaatat ttttaagggc tttttaataa tggttctttt gaaggtagct
      781 aaatatctaa aattttatat attaaattta aagaaaatat aaagatggaa gatacaacca
//
LOCUS       DAEPOZ010000441          823 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.447, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000441 DAEPOZ010000000
VERSION     DAEPOZ010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.447"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..>823
                     /locus_tag="KR440_003752"
     CDS             <1..>823
                     /locus_tag="KR440_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBH0700701.1"
                     /translation="STIHQMLEAELDDHLGYDRYDNKNKNTKNSRNRYRNKNVKSDFG
                     EITLNIPRDRNGDFEPKVIRNYENDISGIENQVIGMYSKGMSTRDISSHLKSIYGIDI
                     SHSLIAKITDRVLPLAHEWQSRALDMVYPIVFMGAIHYKVRSEGRIINKAAYIAIGVN
                     LEGITEVLGIWIGENESSKYWLKVLTDVKNRGVKDILITSVDGLVGFSDAIKAVFPDT
                     EIQRCVVHQIRNTLNYVSYKNRKEFAKDLKKVYAAITEEVALSELSLLEEKWGNQY"
BASE COUNT          331 a           89 c          161 g          242 t
ORIGIN      
        1 agtacaattc atcaaatgtt agaagcggag cttgacgacc atttaggtta tgatagatat
       61 gacaataaga ataaaaatac taaaaactct agaaatagat atagaaataa aaatgttaaa
      121 tcagattttg gagaaataac tttaaatatt ccaagagatc gaaatggaga ttttgaacca
      181 aaggtaatta gaaattatga aaatgatata tcaggaatag agaaccaagt gattggaatg
      241 tactcaaaag gtatgtctac tcgagatata tcaagccacc ttaagagtat ttacggaatt
      301 gatatatctc attctctaat agctaaaatt actgatagag ttctaccttt agctcatgaa
      361 tggcaaagca gagctcttga tatggtttat ccaattgtat ttatgggtgc tatacattat
      421 aaagttagaa gtgaaggtag aataataaac aaagctgcat atatagccat aggtgttaac
      481 ttagaaggta taacggaggt cttaggaata tggattggag aaaatgaaag ttcaaagtat
      541 tggctaaagg tgcttacaga tgtcaaaaac agaggagtta aggatatatt aataacttct
      601 gtagatggat tagttggatt ttctgatgca attaaagcag tatttcctga tactgaaata
      661 caaagatgtg ttgtacatca aattagaaat actttaaatt atgtatcata taaaaataga
      721 aaagaatttg caaaagattt aaagaaagta tatgctgcaa ttacagagga agtagcatta
      781 agtgagctat cgctcttaga ggaaaaatgg ggtaatcaat atg
//
LOCUS       DAEPOZ010000442          802 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.448, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000442 DAEPOZ010000000
VERSION     DAEPOZ010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.448"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            346..651
                     /locus_tag="KR440_003753"
     CDS             346..651
                     /locus_tag="KR440_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700702.1"
                     /translation="MPSMIMEVVGVLFIAIGLILTFIEIKGNFPSFYGFGQNSIGKNA
                     VGIIAAGIALIFFRNGIDSIDSIATYGVFLAIELVVIVAYFLIRKKFTKEDKHLRTN"
BASE COUNT          340 a           73 c          100 g          289 t
ORIGIN      
        1 ttataaataa aaaccaagct tatagattag agaatgaatt aattataaat aaaaaccaag
       61 cttatagata agaagtccat gcttataagc cagaaaatga atactttaat agattaaaaa
      121 atacatattt gtgaattata gaatgaataa ctccataaac tagaaaattt ataataatat
      181 ctatatgaaa attagaatta ttttatcaca aaaaaattaa gttaaataaa tatgaaatta
      241 cttccaaata tacttccaaa aacatgtaat cgcatatata atataattat aaaaataata
      301 aatattttta tagtagtagt taattattaa taaggagggg tcaaaatgcc tagcatgatt
      361 atggaagttg taggagtact atttatagct attggattaa tcttgacatt cattgaaata
      421 aaaggaaatt tcccttcttt ttatggtttt ggtcaaaatt ctatagggaa aaatgctgta
      481 ggtattatag cagcaggaat tgctttaatt ttctttagaa atggcataga tagcatagat
      541 tctatagcta cttatggtgt atttttagca atagaacttg ttgtaatagt tgcatatttc
      601 ttaataagaa aaaaatttac taaagaagat aaacatttaa gaactaatta actaaattat
      661 ctactataaa attggaaaat taaaaacaaa taaaagtttg tttttaatta tttaaaatcc
      721 caatttgatt atttttaaag aaactatatt ctgtataaaa ctagaatgtg gtttcttttt
      781 tatgtataaa atatttttta gc
//
LOCUS       DAEPOZ010000443          801 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.449, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000443 DAEPOZ010000000
VERSION     DAEPOZ010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.449"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..>801
                     /locus_tag="KR440_003754"
     CDS             <1..>801
                     /locus_tag="KR440_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HBH0700703.1"
                     /translation="NKIRKIFKLYALEISKNFERDCLNSFQNLKQISDRGLSLGEKYI
                     EESFKKGIETIVSFGIITIDIDTFKSVYCEKYLDFKRLFNNLNKTFIIPNKNKKNGHA
                     SFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITAVHSYIDEESIKKSNALFNNY
                     KDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDFSKEIERLTEFLGYDIKEYKA
                     SLMDIYINELMESDENDVENAKEKIEKYARYIGCSDENLYVARVNAIYT"
BASE COUNT          338 a           62 c          140 g          261 t
ORIGIN      
        1 aataagataa ggaaaatctt taaattatat gctttagaaa tatctaaaaa ctttgagaga
       61 gattgtttaa atagttttca aaatctaaag caaatatctg atagaggttt aagtttagga
      121 gaaaaatata tagaagagtc ttttaagaaa ggaattgaga ctatagtaag ttttggaatt
      181 ataactatag atatcgatac ctttaaaagc gtttattgtg aaaaatattt agattttaag
      241 agacttttta ataacttgaa taaaactttt attataccaa ataaaaataa aaaaaatggg
      301 cacgctagct tcttagaaat agaatcgaca ataaagaaaa tgagcaatta tttatacaat
      361 gattgtttta aaattcatta tgctgttgta gatgccctta tagaaaatcg tataactgcg
      421 gtacattctt atattgatga agaatctata aaaaaatcta atgccttatt taataattat
      481 aaagatggtt ttattgggaa ggctgacgaa tgcaaagtag taaaacaaat aataacttta
      541 aatccatata gggaagatgt atacgaattt ttggtaaaag aagatggaga ttttagtaag
      601 gaaattgaga gattgactga atttttaggc tatgatataa aagaatataa agcgtcatta
      661 atggatattt atataaatga gttaatggag agcgatgaaa atgatgttga aaatgcaaaa
      721 gaaaagattg aaaaatatgc tagatatatt ggatgtagtg atgagaattt atatgtagca
      781 agagttaatg ccatatatac t
//
LOCUS       DAEPOZ010000444          770 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.450, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000444 DAEPOZ010000000
VERSION     DAEPOZ010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.450"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            19..>770
                     /locus_tag="KR440_003755"
     CDS             19..>770
                     /locus_tag="KR440_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBH0700704.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVVGNASNLKLYTEDLIIDKLLDKIFRDKK"
BASE COUNT          347 a           60 c          135 g          228 t
ORIGIN      
        1 actgagggag gtggggatat gagagaacgc attattcttg aaacattaaa aaatagtagt
       61 ggttatctaa acatagatta ttttgcagat aagttggggg tatctactcg aactatcaga
      121 aatgaaataa aaaagataga aactataaaa gaatgtaatg gttttaaatt agaatataag
      181 tctaaattag gatatattct aaatattaaa gaccaagata aattcgagca ttatctaaga
      241 aacttaccct cagatttagt tgaaaatcca gaacaacggt tggagtctat aatagtagag
      301 cttttagtaa atgaaggtta taaaactata gaacagttaa gtaaaaaatt tctagtaagt
      361 tcatcccaaa taaaaaatga tttaaaaaag atagatgaaa agataaagga tacagaatta
      421 aaactagagc gaaaggctca ttatggaatt aaaatagaag gttctattaa agaaattcaa
      481 agtatattgg tggatagtta ttttagaggg aatagaaata taacagagta caggaataag
      541 tttatagata atttaaaatt agctaatatt agaagtacaa taaaaaatgt acttaatgaa
      601 catgatttag aagcaaattt aacagaactt gaggaaatat tagcacaaat tattatattg
      661 tatataagag ttagtatgag agtagtggga aatgctagca atttaaaatt atatacagaa
      721 gatttaatta tagataaatt attagataaa atttttagag ataaaaaata
//
LOCUS       DAEPOZ010000445          770 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.451, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000445 DAEPOZ010000000
VERSION     DAEPOZ010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.451"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
BASE COUNT          307 a          108 c           42 g          313 t
ORIGIN      
        1 taaatttttc tagaaaaacc tttttagatt tatctactct aataaatata taattttttg
       61 tactttataa gtaactataa cttctgctaa agtaaagatg tattgggagt acatctttac
      121 tttcccatta tagaagttaa aagcatacac cataaaatca atcatccaat acaaattata
      181 tatttcctat gtctaactta atttacaaga atcattctat atgcatattc tgcaatccac
      241 taaacatgct ctaaaccttt actattttat acattctcta taaatcatac aaatcatata
      301 taaaatcccc ctaatatgtt taaaccacct ataaaataac accaatattt agaatattca
      361 atatcaactg cataactggt caataaaaac gtataactag acaaatttca atatttatat
      421 aaataataca agaaaaacaa gcattttatt tatataaatt ttttattttt tgtttatatt
      481 aaaaaactat attgattaat ttattttatt tagtaattat gaaaattcgt cttagaatat
      541 attctatatt tgtagattag ctttaatatt tttaatttca caaaaattaa actcccaata
      601 tttttatcca cttttatctc attaattaat tatattattt ttatgctttt tacaattttt
      661 cctatttaaa tatatattaa tataatcctc tttcataaaa taaatattac tcttattaat
      721 taaaaaaatt ataaccactt ttgattacta atcaaaagaa aatatctaag
//
LOCUS       DAEPOZ010000446          752 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.452, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000446 DAEPOZ010000000
VERSION     DAEPOZ010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.452"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..>752
                     /gene="tuf"
                     /locus_tag="KR440_003756"
     CDS             <1..>752
                     /gene="tuf"
                     /locus_tag="KR440_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBH0700705.1"
                     /translation="ELLELVEMEVRDLLTEYDFPGDDTPIVRGSALMALEDPKSEWGD
                     KIVELFEQIDEYIPAPERDTDKPFLMPVEDVFSITGRGTVATGRVERGVLKVQDEVEL
                     VGLTEAPRKVVVTGVEMFRKLLDQAQAGDNIGALLRGVQRNEIERGQVLAKTGSVKAH
                     TKFTAEVYVLKKEEGGRHTPFFDGYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTME
                     VDLINSIVVEEGLRFSIREGGRTVASGVVATI"
BASE COUNT          310 a           94 c          187 g          161 t
ORIGIN      
        1 gagttattag agttagtaga gatggaagta agagatttat taacagaata tgatttccca
       61 ggagatgaca ctccaatagt aagaggttca gcattaatgg cattagaaga tccaaagagc
      121 gagtggggag ataagatagt agaattattc gagcaaatag atgagtatat accagctcca
      181 gagagagata cagataagcc attcttaatg ccagtagagg atgtattctc aatcacagga
      241 agaggaacag ttgcaacagg aagagtggaa agaggagtac taaaagtaca agacgaagta
      301 gaattagtag gattaacaga agcaccaaga aaagtagtag taacaggagt agagatgttc
      361 agaaaattat tagaccaagc acaagcaggg gataatatag gagcattatt aagaggagta
      421 caaagaaacg agatagaaag aggacaagta ctagcaaaga ctggatcagt aaaggcacac
      481 acaaagttta cagcagaagt atatgtactt aaaaaagaag agggtggaag acatacacca
      541 ttctttgatg gatatagacc acaattctat ttcagaacaa cagacgtaac aggagcttgt
      601 aagttaccag aaggaataga gatggtaatg cctggagata acgtaacaat ggaagtagac
      661 ttaataaact caatagttgt agaagaggga ttaagattct caataagaga aggtggaaga
      721 acagtagctt caggagttgt tgctacaata at
//
LOCUS       DAEPOZ010000447          737 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.453, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000447 DAEPOZ010000000
VERSION     DAEPOZ010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.453"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..>737
                     /locus_tag="KR440_003757"
     CDS             <1..>737
                     /locus_tag="KR440_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HBH0700706.1"
                     /translation="SILTIFAYSNIKYNNNKLIYSNMIDKKTQNSVKEILKENKINEK
                     DIDTFIKAVNNYNKLQVKILQNNINISKSGYSSINAKQVPYNLEKLQDNWVKKFPDYM
                     DVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNKDAKFSTKDVEKFINFFSA
                     IPAKDTDDTIKIAEQIKNEWKKRKISFKNNKNISIINGFLRYPETKNVFIGHTGICIK
                     TKNGILFLEKYGVTSPYQVTKFKNKKG"
BASE COUNT          342 a           80 c           85 g          230 t
ORIGIN      
        1 aagtatactt acgatatttg catattcaaa tattaaatat aataataata aattgatata
       61 ttctaatatg atagataaaa aaactcaaaa tagtgtaaag gaaatcctaa aagaaaataa
      121 aattaatgaa aaagacatag atacatttat taaagcagtc aacaattata ataaattgca
      181 agttaaaata cttcaaaata atataaatat atctaaatct ggatattctt caatcaatgc
      241 aaaacaagtt ccatataacc tagaaaaatt gcaagataat tgggtgaaaa aattccctga
      301 ttatatggat gtaaattgta gaataactgc ttttagactt tttaaagatt ttataaattc
      361 aaataagaaa tttactggag actctattga tttgaatgtc gacttagaca ctataatgaa
      421 taataaagat gctaaattta gcaccaaaga tgtagaaaaa tttatcaact tcttttctgc
      481 aataccagct aaagatacag atgatacaat caaaattgca gaacaaataa aaaatgaatg
      541 gaaaaaaaga aaaatatctt ttaaaaataa taaaaatata tctattataa atggatttct
      601 tcgttatcca gaaactaaaa acgtatttat aggtcataca ggcatatgta ttaaaactaa
      661 aaatggcatt ctatttttag aaaaatatgg tgttacatca ccttatcaag ttactaaatt
      721 taaaaataaa aaaggtg
//
LOCUS       DAEPOZ010000448          723 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.454, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000448 DAEPOZ010000000
VERSION     DAEPOZ010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.454"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..>723
                     /locus_tag="KR440_003758"
     CDS             <1..>723
                     /locus_tag="KR440_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HBH0700707.1"
                     /translation="KNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGD
                     STTMYPSQNISDEIKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRT
                     VPYISKVSGVPIVDLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEV
                     SLGPEMKSTGEVLGVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAK
                     DMKELGYTFVATEGTAKSLRENG"
BASE COUNT          292 a           75 c          154 g          202 t
ORIGIN      
        1 aaaaatccag tacttataga taaatatcta actggtagag aaatagaagt agatgctata
       61 tgtgataagg aagatatatt gatacctggt ataatggaac atttagaaag agctggagtt
      121 cactctggag atagtacaac aatgtatcca agtcaaaata tatctgatga aataaaagaa
      181 aagatagtag aatacactaa aaaaatagct ttagacctta atgtgcttgg tatggttaat
      241 atacaattta tagaatttca aaatgaatta tatataattg aggttaaccc tagagctagt
      301 agaacagttc catatataag taaagtaagt ggagtaccaa tagttgactt ggctactaag
      361 tgtatgttag gagctaaatt aaaggattta ggatatggta caggagttta caaagagcca
      421 aaattagtat cagtaaaagt accagtattc tcaatgtcta aattatctaa agttgaagta
      481 agtctaggac ctgaaatgaa gtctacagga gaagtcctag gtgtaggaga aaatttagaa
      541 gaagccttat ataaagggtt cttggcagca ggtagacata tgtctgatga aagaggagta
      601 gtacttgcta ctgtaaataa tcatgataaa gatgagttta tagagatagc aaaagatatg
      661 aaagaattag gatatacttt tgttgctaca gaaggaacag cgaagagttt aagagaaaat
      721 gga
//
LOCUS       DAEPOZ010000449          723 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.455, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000449 DAEPOZ010000000
VERSION     DAEPOZ010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.455"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
BASE COUNT          229 a          117 c           43 g          334 t
ORIGIN      
        1 tttgagcccc atcattatat aaaattaacc tacttgatat cttaatttaa tatatgtaca
       61 ctattcatat cttaatagtc aatattattc atttttaata gctcttttta gatgtcagaa
      121 ttattatatt ttgaaattta ttaaattttt gcattaaaaa aactagaaat taatttaagt
      181 cttgaattac ttataacttt ctacgtatct acctttatat tcttactctg attttatatc
      241 taatattaac tcttcatcaa agctattaaa tatacctcta attgagtcaa ttatttcact
      301 tacttcacct ttaattttta tattaacttc aaccatttca aatctcatct ttttatctta
      361 taatttctat aagttttttt atttattgtg ttgcttactt tgaccagcac ttttttattt
      421 aatagtctaa atgatatttt ttaccagttt taatatcctt aaatactata tttactaaaa
      481 acttataatt tcttttaata cattcttatt actaataaca atactcaaca attttatcgc
      541 cttttttcaa atatgattta acttttttat tcctaattat ttaaaaatag ttgtccaatt
      601 ttcttctatg agaaagtgct aagtattagc aacttctcta ttctcttaac tttctactgt
      661 ctcaactttc ttcatagcta tattaatagc tacttcttta gctacttcat tgaactcttc
      721 cca
//
LOCUS       DAEPOZ010000450          722 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.456, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000450 DAEPOZ010000000
VERSION     DAEPOZ010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.456"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..>722
                     /locus_tag="KR440_003759"
     CDS             <1..>722
                     /locus_tag="KR440_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700708.1"
                     /translation="NFKEKYKIIKTRFEIDNELDNSTKLYKEVKQELNKMKLQVQDLE
                     NNKKQLEKELEDLQNYKDIIFEEEILTDLERNGIKPIEGMKYLLTLDGDLEYKNNLIS
                     KNPLLPYSIIITNKEYQKLKQINLKNKTKISMPIFVRDNIEDTFITTSSNLVISNGLN
                     ILSSFDNKALDPEKREKDMNLIKEQINSIRDKVEQYKAEIEILEEFKLLTNKIDFTAE
                     DLSIDVKIENYKKIIFEINNKI"
BASE COUNT          359 a           76 c           87 g          200 t
ORIGIN      
        1 ataattttaa agaaaaatat aaaatcatta aaacaagatt tgaaatagac aatgaattag
       61 ataactctac taaactatat aaagaggtaa aacaagagct taacaaaatg aaacttcaag
      121 tacaggactt agaaaataat aaaaagcaat tagaaaaaga gctagaagat ttacaaaatt
      181 ataaagatat aattttcgaa gaagaaatat taacagatct agaaagaaat ggaataaaac
      241 ctattgaagg catgaaatat ctattaacct tagatggaga tttagaatac aaaaataatc
      301 tcatatctaa aaatccattg ctaccatatt ctataataat tacaaataaa gagtatcaaa
      361 aactgaagca aataaactta aaaaataaaa ctaaaatctc aatgcctata tttgtaagag
      421 ataacataga agatactttt ataactacat ctagtaacct tgtaattagt aatggattaa
      481 atatactatc ttctttcgat aacaaagctt tagatcctga aaaaagagaa aaggatatga
      541 atctaatcaa agaacaaata aatagtattc gagataaagt ggagcaatat aaagcagaaa
      601 ttgaaatatt agaagaattt aaacttctaa ctaataaaat tgattttaca gctgaagact
      661 tatccataga tgtgaaaatt gaaaattata aaaaaattat atttgaaata aacaataaaa
      721 tt
//
LOCUS       DAEPOZ010000451          700 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.457, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000451 DAEPOZ010000000
VERSION     DAEPOZ010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.457"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..423
                     /locus_tag="KR440_003760"
     CDS             <1..423
                     /locus_tag="KR440_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBH0700709.1"
                     /translation="TISKKYKMRSVNPLKAWVKEYNLHKEIKETPKGGLFMETRKTTL
                     DERVEIVKDCIEHDFNYNLIAKKYFVSYSQVRRWTLKYKENGIVGLEDRRGKAKKEDE
                     LTQLDEIKLELKIVKAEKHKLQLENAFLKKLKNIERGW"
     gene            417..>700
                     /locus_tag="KR440_003761"
     CDS             417..>700
                     /locus_tag="KR440_003761"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBH0700710.1"
                     /translation="MVITLSKNENKYKAIQSLFNEKKASLMQLCEIAKVNRSGYYKWL
                     NRDKSNLELENIKLATLITNIYEEKKGVFCSLRMTLQLNREYKLNVNHKRV"
BASE COUNT          307 a           78 c          126 g          189 t
ORIGIN      
        1 actatctcaa aaaaatataa aatgaggtct gttaatccac taaaagcttg ggttaaggag
       61 tataatcttc ataaggagat aaaagaaacc ccgaagggag gactctttat ggagactaga
      121 aaaacaacat tagatgaaag ggttgaaatt gttaaagatt gtattgagca tgattttaat
      181 tacaatttaa tagcaaaaaa atactttgtt tcatacagcc aagttagaag atggactctt
      241 aaatataaag aaaatggtat agttggttta gaagaccgaa gaggtaaagc aaaaaaagaa
      301 gatgagttaa ctcaattaga tgaaataaag ttggaattga aaattgtaaa agcagaaaaa
      361 cacaaattac aactggagaa tgcattctta aaaaagttaa aaaacataga aagagggtgg
      421 taattacact atccaagaat gaaaataaat ataaagctat tcaatcctta ttcaatgaaa
      481 aaaaagcttc gcttatgcaa ttatgtgaaa tcgctaaagt aaatcgttct ggatactata
      541 aatggctcaa tcgagataaa agtaatttag aacttgaaaa cattaaatta gcaactttga
      601 taactaatat atatgaagaa aagaagggag ttttttgctc tttaaggatg accttgcagt
      661 taaacagaga gtataaactt aatgtcaatc acaagagagt
//
LOCUS       DAEPOZ010000452          696 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.458, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000452 DAEPOZ010000000
VERSION     DAEPOZ010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.458"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            32..>696
                     /locus_tag="KR440_003762"
     CDS             32..>696
                     /locus_tag="KR440_003762"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HBH0700711.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YIL"
BASE COUNT          283 a           73 c          116 g          224 t
ORIGIN      
        1 gtctaattaa tttaaattag gagagtaata tatgaataaa aaacttttag aaatactatc
       61 aaaatactat ggatatacaa gttttagaaa aggtcaggaa tctataataa attctatctt
      121 aagcgaaaaa gatgtgttag ctattatgcc aacaggaggg gggaaatcaa tatgttatca
      181 aattccagct ttgattttag atggaatgac aatagtaatt tctccactta tatcacttat
      241 gaaagaccaa gtagatgcct taaagactat gggaattagt gctgctttta taaatagttc
      301 attaagcagc aaagaattta atgaaatatt aaataatatt agaaaaaata attataaaat
      361 actctatatt gctccagaga gattagatgc acaagagttt attgagctaa taaataataa
      421 caatgtaagt caagttgcga tagatgaagc acattgtgta tctcaatggg ggcatgattt
      481 taggttgagt tatagaagga tttctgattt tataaaaaat ttgcctaaaa gacctatagt
      541 tacagcattt actgcaacag cttcagaaga ggtaagaact gatataataa atttactgtg
      601 tttagaaaat ccagattatt atataactgg atttgataga gagaatttat caataaatat
      661 agtaaaatca tctagtaaaa ataagtatat tttaga
//
LOCUS       DAEPOZ010000453          676 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.459, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000453 DAEPOZ010000000
VERSION     DAEPOZ010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.459"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            251..>676
                     /locus_tag="KR440_003763"
     CDS             251..>676
                     /locus_tag="KR440_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBH0700712.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGDENEVDKIIKVEKQNIIKNKNLTIF"
BASE COUNT          288 a           37 c          109 g          242 t
ORIGIN      
        1 aaaagattat aaaaataaag agatatttag ataattagtg taaataggtt ttaaatcaac
       61 ataataactt aaattatatg gtataataaa ttaaataatt gttttaattc aaaatttttt
      121 gttattttaa attatttcaa aactcttagt tttagtgatt ttatttatga tatttatggg
      181 attaaaagat ttcaatagat atttttggta agtatttaag aagtctttaa ctttatagga
      241 gtgataattt atgctaatag gtacatctaa attaagcaga aatgttgata ctgcatgtgt
      301 agaagaagta gggattccaa tgattgtatt gatggaaaat gcggttatat ctgcaatgaa
      361 aaatatggat atagacatgt ataatagtta tacagttgta tgtggtgttg gaaataatgg
      421 aggagatggt cttggaatag caagacatct aaatacatat ggaaaagaag taaacgtatt
      481 tttagtaggt aatcttgaaa aactaagtga atgctctaaa ataaattaca atgtactttt
      541 aaatatggga attaatatta taaatattaa taaaaatgat ttaagtgaaa aaaattatgg
      601 agatgaaaat gaagtagata aaattattaa agttgagaaa caaaatatta taaaaaataa
      661 aaatttaaca attttt
//
LOCUS       DAEPOZ010000454          670 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.460, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000454 DAEPOZ010000000
VERSION     DAEPOZ010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.460"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..229
                     /locus_tag="KR440_003764"
     CDS             <1..229
                     /locus_tag="KR440_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0700713.1"
                     /translation="SENKDELIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLK
                     DKHRFISRNPNCIVDNTSIEEIMLFYVRGDK"
     gene            229..>670
                     /locus_tag="KR440_003765"
     CDS             229..>670
                     /locus_tag="KR440_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBH0700714.1"
                     /translation="MKGLILKDLLNLKGNVKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERAR"
BASE COUNT          234 a           61 c          103 g          272 t
ORIGIN      
        1 tagtgaaaat aaggatgaat taattgatac tatgggagtt ttaaaatgta aacctagtga
       61 ttttgataac ctttcaagag aggattattc atattatcgt aaaagccaat ttggatatga
      121 agtacttctt aaagataaac atagatttat atcaagaaat ccaaattgta ttgttgataa
      181 tacgtctata gaagaaatta tgttatttta tgtgagaggt gataaataat gaaaggttta
      241 attttaaaag atttacttaa tttgaaagga aatgtaaagt ttatactttt attcatcatt
      301 atgtttgggt ttatgtcttc tttaggagat ggaaatgtta ataactttat aggtgttatt
      361 atagttttat gtactacaat gattgtttca acattttcat atgatgattt aaataaatgg
      421 gatagttatg tacttacaat gcctattaat cgcaatgata ttgtattaag taaatattta
      481 actatgttaa tatttagctt tataggtgtt cttgtttcac ttattgttag tgtaactatt
      541 ggttatttta aaaatacact tatattaaat gaaacactac ttattaatgc actaatctta
      601 tcaatttcag tatgttttgg tagccttatt ctaccactta tttataaatt tggtacagaa
      661 agagctcgtt
//
LOCUS       DAEPOZ010000455          647 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.461, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000455 DAEPOZ010000000
VERSION     DAEPOZ010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.461"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..530
                     /locus_tag="KR440_003766"
     CDS             <1..530
                     /locus_tag="KR440_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBH0700715.1"
                     /translation="TNFTFKGEEGLDIYTYKWEDENIKKPKAVIQIAHGMAETAQRYE
                     TFAKVLTKNGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIKKEN
                     EDLPIYLFGHSMGSFASQRYIMDYSNNLSGLILCGSNGKQGIILNLAHLIINREIKKY
                     GRRSKSNKINNLIKK"
BASE COUNT          262 a           78 c          121 g          186 t
ORIGIN      
        1 gtacaaactt tactttcaaa ggagaggaag gtttagatat atatacatat aaatgggaag
       61 atgaaaatat aaaaaaacca aaggcagtta tccaaattgc acatggaatg gctgaaactg
      121 cacaaaggta tgagactttt gccaaggtac ttactaaaaa tggttatata gtatatatca
      181 atgaccatag aggtcatgga aaaactgcaa agataataga aaatgttgga catttagctg
      241 aaaaagaagg atttagatgt cttgtagagg acatgtatac tttaacaaat attataaaga
      301 aagaaaatga agatttgcca atttatctat ttggacacag catgggttca tttgcaagtc
      361 aaagatatat aatggattac agtaataatt tatctggtct tatactatgt ggttcaaatg
      421 gaaagcaagg aatcattcta aatcttgctc atctgataat caatcgtgag attaaaaagt
      481 atggaagacg ttctaaaagt aataaaataa ataatttgat taaaaaatag ctatgcacac
      541 ttgtgacttt agtcatgagt tagtagctaa aatagtcagc atatagggaa acctatatgt
      601 agagatagga tagaacctat ccaatactac ttgaattgct ggaaacc
//
LOCUS       DAEPOZ010000456          639 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.463, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000456 DAEPOZ010000000
VERSION     DAEPOZ010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.463"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(110..472)
                     /locus_tag="KR440_003767"
     CDS             complement(110..472)
                     /locus_tag="KR440_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBH0700716.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
BASE COUNT          222 a           99 c           39 g          279 t
ORIGIN      
        1 tgaatttttt tgatttgaat ttgctaatta atatttataa agattagatt taatacataa
       61 atttataggt gtattaatta ataattttaa tcaacacacc tatctcttcc taatcttctt
      121 ttagcaaagt acttttccat acatcttctt taaagcccaa taatacattt ttaccatcca
      181 aaagtaatgg tcttttaata agcataccat cacttaccaa tatttctaaa agtttatcat
      241 cagattcagt ttttacaata tctttaagac ccaattctct atatttcata ccacttgtat
      301 taaaaaattt ctttatatca tatccactag tcttataaat tgattttaac tcctctttac
      361 taggagggtt ttgaaccata tctatatctt caaattctaa attattttcc ttaagccaat
      421 ttttagcttt tttaacagta gagcattttg tatatccata taatttaatc attttataaa
      481 aattcctttc taatttttta ttgttatacc cataaattgt atactataac caatttatat
      541 cataatcatt ataagctttc aacataaaaa attatcatat ttcaaattat taaacattta
      601 ttcacattat atatttaaat agtcctttta tttttatta
//
LOCUS       DAEPOZ010000457          638 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.464, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000457 DAEPOZ010000000
VERSION     DAEPOZ010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.464"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..>638
                     /locus_tag="KR440_003768"
     CDS             <1..>638
                     /locus_tag="KR440_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBH0700717.1"
                     /translation="LEKKHPQPLLDLEIFRNFKFSLNLICALTSFICIASSSILIPFY
                     LQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKIILIGLLVMSFGFFLMS
                     RLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKLGVVGSINSLVRNLGQV
                     IGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYIIVTLVCLIGA"
BASE COUNT          211 a           83 c           90 g          254 t
ORIGIN      
        1 attagaaaaa aaacatcctc aaccgcttct tgatcttgaa atttttagaa attttaaatt
       61 ttcattgaat ttaatttgtg ctcttacatc attcatttgt attgcatcat caagtatttt
      121 aattccattt tacttacaaa gtactatgaa acttccccct attcaagcag gtttatttat
      181 gatactatca cctttgattt tagccatttt ttcacctata tttgggaata tatcagataa
      241 aatcaagtca gaaaaaatta ttttaattgg tcttctcgtt atgagttttg gattctttct
      301 aatgtcaaga ttgaaagaaa gttctgccct tattttattt gtaatttata tatcgataat
      361 atcaatagga caagctatat ttcaacctgc taacaatgct ttgataatgt caagctgttc
      421 tagaagtaag ctaggagttg taggaagcat aaattccctt gtaagaaatt taggacaagt
      481 tataggtatt actatttcta caacactttt atataatttt atgagtatta aagctggata
      541 tagggtcaat gattatgtga ttaataatga taaaatattt gtatttggaa tgagaaatgt
      601 ttatattatt gttacacttg tttgcttaat aggtgcaa
//
LOCUS       DAEPOZ010000458          632 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.465, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000458 DAEPOZ010000000
VERSION     DAEPOZ010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.465"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..188)
                     /locus_tag="KR440_003769"
     CDS             complement(<1..188)
                     /locus_tag="KR440_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBH0700718.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIG"
     gene            395..>632
                     /locus_tag="KR440_003770"
     CDS             395..>632
                     /locus_tag="KR440_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700719.1"
                     /translation="MLVRVKIVIGATIRYLIKHKVNYCNKKSLDKLELKYGNIDREKA
                     VKLEIFYQYLIELEYIEMGLLIRRLDTAITSLALV"
BASE COUNT          238 a           66 c           77 g          251 t
ORIGIN      
        1 attcctattt ctcttaatga ttgtaatata tcaagtgtat catattgata aactgagtaa
       61 tatctataat tatttttatc tttatattca ggagaaaaaa tacctatttt gtcatagaaa
      121 attaatgtct gcttagatat accacacaat ttagcaaatt cacctgttgt aaaatactgt
      181 ttttccaagg gttatacact ccttttcttt ttgttgtcta tatagttact atattcttta
      241 ttatttacta aatcaagtag ttattatttt agaaaatcaa tatggttttg attttaaagc
      301 ttaaatatca gttagatgtc agttataaat attttatcca ttcctctcat aaaaaagata
      361 tcccctctta acttttggat ggaatattat atttatgctt gttagagtga aaattgtaat
      421 aggtgctaca ataagatatt taattaaaca taaagttaat tattgtaata aaaaatcttt
      481 agataaatta gagttaaagt atggaaatat agatagagaa aaagctgtta aactagaaat
      541 attttatcaa tatttaatag aacttgaata tatagaaatg ggtttattaa taagaagatt
      601 agatactgca ataacatcat tagcattagt at
//
LOCUS       DAEPOZ010000459          625 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.466, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000459 DAEPOZ010000000
VERSION     DAEPOZ010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.466"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
BASE COUNT          266 a           42 c           86 g          231 t
ORIGIN      
        1 tttcatataa tatttcaagt ttgtataatg taacaattaa accaaaagac tcattgtata
       61 tttgaaatta agttttttat agtttatagg tgttttatgt ggattacgaa tgatatagta
      121 tcgatacatt atttaatcga ttcttattcc aagaaacaat taatttatag aaattcttaa
      181 ttgttacaat ataaatatct agataagtta taagatactg ataaataatt tataaagttt
      241 attaatctaa tattgtgtgt gaatgttaag tggtcaagta gtctaaaaaa tgaaaaagat
      301 ttaaaaaagt gttttaacta aacacataag tgttaaattc atgtataagt ataggttaga
      361 tacataagaa ataattatgt aatatctatc tttgtaaaaa aatattaaat tttgtaattt
      421 gtggataaat atgtttataa tgtggataat atttaatgta ataagttaaa gcaatatgaa
      481 tcaactgttt ataatgtgga taatattata aatgtatgaa taattgcagt atttaaaaac
      541 aaaaaaagtt aaaaaagttg ttgactctaa aagaaaaagt tggtatagta atacttgtcc
      601 tcaagaaaag ggcaaacaaa taaag
//
LOCUS       DAEPOZ010000460          624 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.467, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000460 DAEPOZ010000000
VERSION     DAEPOZ010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.467"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(118..597)
                     /locus_tag="KR440_003771"
     CDS             complement(118..597)
                     /locus_tag="KR440_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBH0700720.1"
                     /translation="MPNKLIKNLLSGILQILFFLLGLVIVVGGFKSFMYLCFSGEATL
                     QGTISGILMFILGVSYFIIIKSLIEVLSSSEHSLFVKDNVKRFRIIGYLLLLNSIMEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT          244 a          104 c           49 g          227 t
ORIGIN      
        1 taatctttta attttagcat tatagtttat gtattatacc ctaatatatt ttattttaaa
       61 ttactagtta atcactcttt tctggttatt tatctcctat atattaaagt gtcacaccta
      121 aatagttaaa tcattatcct cttttatctt tattgccttt acaaatccat ctgcaattac
      181 aaaactcata agagaagtta taaaatatac aaatactgga acagtaaaat aaaatccaaa
      241 tcctaaatct aggaatctca ttccttttcc agtagtacca aaagttgaaa taaactccat
      301 tatagagttt aaaagcaaaa gatatccaat aatcctaaat ctttttacat tatcttttac
      361 aaataagcta tgttcagaac tacttaatac ttctatcaat gattttataa tgataaaata
      421 tgatacacct aaaataaaca ttaatattcc agaaatagtg ccttgtagag ttgcttctcc
      481 actaaaacag aggtacataa aacttttaaa tcctcccaca acaattacta accctaaaag
      541 aaaaaataaa atttgtaata taccacttag taaatttttt ataagtttat taggcatcat
      601 accaactcct ttaaaatatt ttat
//
LOCUS       DAEPOZ010000461          620 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.468, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000461 DAEPOZ010000000
VERSION     DAEPOZ010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.468"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..>620
                     /locus_tag="KR440_003772"
     CDS             <1..>620
                     /locus_tag="KR440_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBH0700721.1"
                     /translation="TDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLKNFIIHLA
                     ITISRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVYLYNHFIT
                     KSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKSILNSKSY
                     NLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYV"
BASE COUNT          244 a           58 c           82 g          236 t
ORIGIN      
        1 tacagataga gaaatagaat tatttaataa tgtagattta atagagcttc aaagggtagt
       61 gttaagtgaa attaataaat ttaatataaa ttttctagat tttaatctaa aaaactttat
      121 tatccattta gctattacta taagtagaat acttgatggt tactgcttag ataatgtttt
      181 agatgttgtt ttaacagatt ttcaatcgaa tacaactgtt gaaaatatat ttaattatat
      241 tgagagtaaa tatactataa ttatttcaaa ggcagacaga gtttatctat ataatcattt
      301 tataactaaa agttctcttt tggataacgt ttccaatagg gtggatacta aaattataga
      361 gtatgttgaa gaaattttag aagtaatcaa caatcaatat acttttgacc ttagaaatga
      421 tagtgtatta tttgatgatt tagtactgca ctttaaatca attctcaatt ctaaatctta
      481 taatttaaat aaggtgaatc cccttataaa tacaataaag agtaattatc ctttagcatt
      541 tgaaataact ttaaatgcta tagaaaaagt ttttaaaaat agtatttact ctttaacaga
      601 agatgaaatt ggatatgtat
//
LOCUS       DAEPOZ010000462          598 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.469, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000462 DAEPOZ010000000
VERSION     DAEPOZ010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.469"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..540
                     /locus_tag="KR440_003773"
     CDS             <1..540
                     /locus_tag="KR440_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HBH0700722.1"
                     /translation="VVIIGGGEVAYRKCKNFLEFNKNVTIVSKQILNKFYDLKGNIKI
                     IKDDYKEIYIKDASVIIAATNNRELNMEIGLYCRAKNKLVNVVDNIEISNFTVPSYIK
                     RGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRKKVIKKYEDKSKRK
                     DVLNMLVTLDLEELKKFDI"
BASE COUNT          271 a           37 c          107 g          183 t
ORIGIN      
        1 gtagttataa ttggtggtgg agaagttgca tataggaaat gtaaaaattt tttagagttt
       61 aacaaaaatg ttactatagt ttctaaacaa attttaaata aattttatga tttaaaagga
      121 aatataaaaa taataaaaga tgattataaa gaaatatata taaaagatgc ttctgtaata
      181 attgctgcca caaataatag agagttaaat atggaaatag gattatattg tagagcaaaa
      241 aataagctag tgaatgtagt tgataatata gaaatatcga actttactgt tccatcatat
      301 ataaaaagag gagatttact tataagtgtg tctactgggg gcaagagtcc ttcattatca
      361 tcaaagataa aaaaagaaat tgaagagaga tatactgaag attatgagga gtatttaaat
      421 gtattaggag atataagaaa aaaggttata aaaaaatacg aagataaaag taaaagaaaa
      481 gatgtgctta atatgttggt aacacttgac ctagaagaac ttaagaagtt tgatatataa
      541 aaaataaaat aaaaaatgtt aaaaaggtgt tgacggatta tttttaagat gatatagt
//
LOCUS       DAEPOZ010000463          597 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.470, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000463 DAEPOZ010000000
VERSION     DAEPOZ010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.470"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            2..286
                     /locus_tag="KR440_003774"
     CDS             2..286
                     /locus_tag="KR440_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700723.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGRICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
BASE COUNT          258 a           49 c           74 g          216 t
ORIGIN      
        1 aatgcttgta tataataaaa gtttttatcc taatgacata tttccaagat tagatttttc
       61 aaaaataaaa aaacagttaa aattgataga taatgacctg tcagattttg gaagaatatg
      121 tataatagaa aaagaacatt atacgataag tgtaaacagt ataggtgaaa taaatgtgta
      181 ttatgattta gagtacgaaa ataaggtgta tagaatagtt tatgagattg aaaagttatt
      241 taaatctcaa gttggaaggt ttagtatatc tacatacaga aattgataat taaaaaagta
      301 gaaattaaaa aacttaatac taaagatata gataaatata taaggtaaca gctagagaga
      361 gttgttaccc tttttaaata ctaagtatat attattctta ttaatttttt gtaaattcac
      421 taaaagacct ttttatagaa tattattttc tttttttatt aaataggtaa atcagtaaaa
      481 atgcaattcc agttccaatt attattccaa tgatattttc tataattaat ttttttaata
      541 taaaagtaaa taataaaaat gctatacata aacctaaaat atatccttta aaatagg
//
LOCUS       DAEPOZ010000464          594 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.471, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000464 DAEPOZ010000000
VERSION     DAEPOZ010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.471"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..587
                     /locus_tag="KR440_003775"
     CDS             <1..587
                     /locus_tag="KR440_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700724.1"
                     /translation="GYKFYKNNDRFKDCYLLARLLLLNLSMDNSQNNQEAFSILFEIN
                     TLYEEYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYE
                     IIIDTKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWKLS
                     ESFKGKFIEAKTVRLDDIKNTKNDLKKIIFNYKF"
BASE COUNT          263 a           47 c           84 g          200 t
ORIGIN      
        1 tgggttataa attttataaa aacaatgata gatttaaaga ttgttattta ctagctagac
       61 ttctactttt aaatctatct atggataata gtcaaaataa tcaagaggca ttttctatat
      121 tatttgaaat aaatactttg tatgaagaat atataggaat tttaatcaaa agtatatggg
      181 acaattcatt tagagaaaca tatatacaag ataagagtaa atttcttttg aaaaatgaac
      241 aaacaggtaa gaaaaacttt aatttaagac cggatatagt tttatatgac ttaaaaaatg
      301 aatatgaaat tataattgac actaaatgga aagctattga agtagattca aatgtttttt
      361 ataggtcaag tgatatatac cagatgtatg catatataac agcatatgaa aatgcaaaaa
      421 gatgtatatt attatatcct tgtattcaaa aagataagaa ttatagttcc tggaaattat
      481 cagaatcatt taagggtaag tttatagaag caaaaactgt gaggttagat gatataaaaa
      541 acacaaaaaa tgatttaaaa aagataattt ttaattataa attttaaaaa taag
//
LOCUS       DAEPOZ010000465          581 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.472, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000465 DAEPOZ010000000
VERSION     DAEPOZ010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.472"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..>581
                     /locus_tag="KR440_003776"
     CDS             <1..>581
                     /locus_tag="KR440_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBH0700725.1"
                     /translation="ISFLYNNKREIFNKLSLSIKSGEKVAIVGESGSGKSTLIKLLIG
                     LLKPSEGTIFIDDYNLLEMNLNSYYNYVTYVSQDSPIFDGTLRENIVFGKTIKSNDII
                     EVLEKVGLKNLYLKLEAGLDTKLGEKGTTLSGGEKQRLALARLWFSDAKIIILDEATS
                     ALDNITEEKVMLNVMEFLKDKTTIMIAHRFNSI"
BASE COUNT          228 a           55 c          106 g          192 t
ORIGIN      
        1 agattagctt cttgtataat aataagcgag agatatttaa taaacttagt ttaagtatta
       61 aaagtggaga aaaagtagct attgttggag agagtggttc tggtaaatca actttaatta
      121 aactattaat tggattatta aagcccagcg aaggaactat atttatagat gattataatc
      181 tacttgagat gaatctaaat agttactata attatgttac atatgtgtca caggattcac
      241 ctatatttga tggaacttta cgtgagaata ttgtatttgg aaaaactata aaaagtaatg
      301 atattattga agtcttagaa aaagtaggtt taaaaaattt atacttaaaa ttagaagctg
      361 gtttagatac taaattaggt gaaaaaggaa ctacattatc aggtggagaa aagcagaggc
      421 tagcattagc aaggctttgg ttttcagatg ctaagataat tatattagat gaagctacat
      481 cagctttaga caatattaca gaggaaaaag ttatgctaaa tgttatggaa tttttgaaag
      541 ataaaacaac tattatgatt gctcacagat ttaactcaat t
//
LOCUS       DAEPOZ010000466          580 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.473, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000466 DAEPOZ010000000
VERSION     DAEPOZ010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.473"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            47..415
                     /locus_tag="KR440_003777"
     CDS             47..415
                     /locus_tag="KR440_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBH0700726.1"
                     /translation="MKKIPKSELLVMNFIWDKGIDKIPAREVANYMEISHGWTKGTTG
                     KVLSRLSDKGFLEFEKDGRNTLYTIKVKHEEYLKFETKDFLELIHKNSIHSFISSLGC
                     GNEISDDDIKELEQWLKKRK"
BASE COUNT          234 a           51 c           89 g          206 t
ORIGIN      
        1 attagacatt tgtctaatat aatcaagtta aaggtgagtg attatattga aaaaaatacc
       61 aaaatccgag ttgttagtta tgaatttcat atgggataaa ggcatagata aaataccagc
      121 cagagaagta gctaattata tggaaatttc tcatggatgg acaaaaggta ctactggtaa
      181 agtactttct agattaagtg ataaaggttt tttagaattt gaaaaagatg gtagaaatac
      241 tttatacact attaaagtta aacatgagga atatctaaaa tttgaaacaa aggatttttt
      301 agagcttata cataaaaatt ctattcatag ctttatatca tcattaggtt gtggaaatga
      361 aatatcagat gatgacataa aagaacttga acaatggcta aaaaaaagaa aataggagac
      421 gtattttatg gaattattta tagaagttat taaatttaat atttacataa gtctactaat
      481 tctttttttc attttattta ctaaaaagat aagcaaatat acttttaagt ttaattattt
      541 tatatgtctt ttaatagtag tcagaatgat gtttatatct
//
LOCUS       DAEPOZ010000467          579 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.474, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000467 DAEPOZ010000000
VERSION     DAEPOZ010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.474"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..>579)
                     /locus_tag="KR440_003778"
     CDS             complement(<1..>579)
                     /locus_tag="KR440_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HBH0700727.1"
                     /translation="NKNFFKKGTIITDTTGIKETLINDVLQIIPDDIDFIFGHPMAGR
                     EKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLILEIGFKRVKKLTSQKHDEII
                     AFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRIANMNEDLWSELFLGNRDNLL
                     KVIENFESEVNLVKEAIFNNDKNKLIEYFKKSS"
BASE COUNT          185 a           99 c           52 g          243 t
ORIGIN      
        1 gatgattttt taaaatactc tatcaattta tttttatcat tgttaaatat agcttctttt
       61 actaaattta cttctgattc aaagttttct ataactttta gtaaattatc tctatttcct
      121 aaaaaaagtt cactccataa gtcttcattc atatttgcaa ttctagttaa atctctataa
      181 ctatctccaa taaatttacc tgtatctcta ccttcttcat cactatttat aagagcaact
      241 gccataacat gaggtaactg agaagtaaaa gctataatct catcatgttt ttgtgaagtt
      301 aactttttta ctcttttaaa tccaatctct aaaattaaat tttcaactaa ctctaaattt
      361 tttatgttgt ttctgccagt tggagtaatt atataatttg cgccattaaa aacttgttca
      421 cttgcaaaat caataccctt tttttcccta cctgccattg gatgtccaaa tataaaatct
      481 atatcatctg gaataatttg caacacatca ttgataagtg tttcttttat tcctgtagta
      541 tcagtaatta tagtcccttt tttaaaaaag tttttatta
//
LOCUS       DAEPOZ010000468          547 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.475, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000468 DAEPOZ010000000
VERSION     DAEPOZ010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.475"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
BASE COUNT          221 a           74 c           59 g          193 t
ORIGIN      
        1 cccagtgcca taatcgataa aattaaactt tgcgtcatta cagatataat acctgacata
       61 aataaaacac ccctttgaaa atattttaaa tggtttttta aatttggttt taaaaaatga
      121 ttttgatata aaaaaaccac acagtatata taactgtatg gcaaaaattc gaataagcca
      181 cacagtataa tccatgtggc tctatattat gatgataaaa taaaaacttt aacaacaatt
      241 actatagcca acatagatac aatccttaaa aatcataatc ctaaggggct tgtatctatg
      301 ttatgacaat ttgtcttaac ttaggtacaa accctaacta aattggctac taaaaaatcg
      361 tacttgtttg tttaataaag tcttcatatt tacctctcct tttaatttaa taattttgaa
      421 aattttgttt tctgttttgt atagtaatta gtttaataca gtaaaaaata aaagtcaact
      481 ataaattttg aaaaattatc aagttatcag aaaattgagt ttaaataaaa aaatttttac
      541 atgtaga
//
LOCUS       DAEPOZ010000469          543 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.476, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000469 DAEPOZ010000000
VERSION     DAEPOZ010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.476"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            298..450
                     /locus_tag="KR440_003779"
                     /pseudo
     CDS             298..450
                     /locus_tag="KR440_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010982.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
BASE COUNT          222 a           44 c           78 g          199 t
ORIGIN      
        1 aattacttta tatttcgtag ttatatataa aattactata aaaaatgaaa tatattaaac
       61 aatcaactaa aaaatgaaat attaataaaa aaaattcaaa ttataaatat taaatgaata
      121 taattaaaac aaatatataa atttaagtat aacactcaca ttttaatgta ttgtgaaatt
      181 tttgttgaac tttatgtacc ataacttata aaatttatgt catgttatat ttgaataagt
      241 aataaaattg tagatgcaaa ttaatctaca aaatttatat gaaatggagt tgatgctatg
      301 acagaattaa tgattataag tgggttagta ctattgattt gtataacttc tagcaaggta
      361 ttgtataaat ttggagtacc aatattattg atttttatct tactagggat gttatttggg
      421 tctgatggaa tggtaggaat ctattttgat taaaaaatag ctacgcacac ttgtgacttt
      481 agtcatgagt tagtagctaa aatagtcagc atatagggaa acctatatgt agagatagga
      541 tag
//
LOCUS       DAEPOZ010000470          513 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.477, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000470 DAEPOZ010000000
VERSION     DAEPOZ010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.477"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..>513)
                     /locus_tag="KR440_003780"
     CDS             complement(<1..>513)
                     /locus_tag="KR440_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBH0700728.1"
                     /translation="KVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGN
                     IYLDLDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIAT
                     TEIGNMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIF
                     SKIYKRVDSIA"
BASE COUNT          166 a           91 c           41 g          215 t
ORIGIN      
        1 gctatagaat ctactctttt atatattttt gaaaatatta cttcataatc taatatttta
       61 tctttatcaa gggctaacat tattattaaa cgaacatctg atatttccca agaaataggc
      121 ttctttaata taccaattgc tattttatta tcaaaaattt ttccaatagc tccatgtggt
      181 atagttacca tattccctat ttctgttgtt gctatttttt ctctctcaaa gtaagatttt
      241 ttcatatcct catctatata gcccttttgt acaagtttac ttgccataaa atctataact
      301 tcttctttcc ttttaaacac caaatcagta aaaaataatt cactatcaaa caggcttttt
      361 atatctaaat ctaggtatat atttcctgtt tctatatatt tttcaattaa tctaatttct
      421 ttctcactta aaattggaga tatatttatt actggcacat ctcctacttc aataggaaca
      481 gtagatatta caaaatcaac atctataact ttt
//
LOCUS       DAEPOZ010000471          501 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.478, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000471 DAEPOZ010000000
VERSION     DAEPOZ010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.478"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            180..>501
                     /gene="tcdR"
                     /locus_tag="KR440_003781"
     CDS             180..>501
                     /gene="tcdR"
                     /locus_tag="KR440_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HBH0700729.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDE
                     NILNM"
BASE COUNT          207 a           35 c           61 g          198 t
ORIGIN      
        1 actttattta ttagaaaaaa attactaatt aattattaaa attaatgtat ttataatgcg
       61 tattttcata taaaatttaa tttatttgcc gattatataa ttataatgac tgatttaatt
      121 ccaatgttgt caaaattttt aaataaatca tcattataaa tataagagag gatgatttta
      181 tgcaaaagtc tttttatgaa ttaattgttt tagcaagaaa taactcagta gatgatttgc
      241 aagaaatttt atttatgttt aagccattag taaaaaaact tagtagagtt ttacattatg
      301 aagagggaga aacagattta ataatatttt ttattgaatt aataaaaaat attaaattaa
      361 gtagcttttc agaaaaaagc gatgctatta tagtcaaata tattcataaa tcattactga
      421 ataagacttt tgagttgtct agaagatatt ctaaaatgaa gtttaatttt gtagaatttg
      481 atgaaaatat cttaaatatg a
//
LOCUS       DAEPOZ010000472          475 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.479, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000472 DAEPOZ010000000
VERSION     DAEPOZ010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.479"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..393)
                     /locus_tag="KR440_003782"
     rRNA            complement(<1..393)
                     /locus_tag="KR440_003782"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          126 a          114 c           75 g          160 t
ORIGIN      
        1 aagtcttctc gtttaacata caggactatt accttctgtg gtgggtcttt ccaaacctct
       61 tcaattacga tacctcttcg ttatgatatg tccgcaaccc caataaagaa aactttattg
      121 gtttgggcta atccgcgttc gctcgccgct acttacggaa tcgaatttct ttctcttcct
      181 tcaggtactt agatgtttca gttccctgag ttcccctcat taagctatgt attcacttaa
      241 tgatacttag acattactct aagtgagttt cctcattcgg aaatcttcgg atcaaagttt
      301 acgtgcaact ccccgaagct tatcgcagct tatcacgtcc ttcatcggct cctagtgcca
      361 aggcatccgc cctgcaccct taataacttg accagttaaa aaggtttgat agattagaag
      421 ctatcaactt cgataatttt aaaaagttat cagctttata tattacaaat tagat
//
LOCUS       DAEPOZ010000473          452 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.480, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000473 DAEPOZ010000000
VERSION     DAEPOZ010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.480"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
BASE COUNT          213 a           33 c           42 g          164 t
ORIGIN      
        1 aataaattta gtaaacctaa attgttgaat attaaaataa gttaatttaa tacataaaaa
       61 ctattttaga tgttattcta ttgctatgtt caataatatc attagtagtt caatttataa
      121 aatgaataaa ttatataaat aagattagaa agtagtaaat agtcttatta aatataagta
      181 ataaaacaat aaatacctag tagatattaa taataaaaaa ataaaatact accaaaataa
      241 acttgatagt attttattta aaatacttaa atacagagac atgtatagta tattttatac
      301 ttctttttta ctatagattt ttgatgatat aaaatatgaa atcacaaata gtaaaatagc
      361 aacaaatgga agagaatata ctagtgtatt aagtataatt tctatagaaa taggtgaact
      421 aatattttct aaaatacttt taaaaactaa ta
//
LOCUS       DAEPOZ010000474          449 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.481, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000474 DAEPOZ010000000
VERSION     DAEPOZ010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.481"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..424
                     /locus_tag="KR440_003783"
     CDS             <1..424
                     /locus_tag="KR440_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0700730.1"
                     /translation="EKKEVCDKNDIELKIGINFSCCEAVELVDICHIFSNILDNAIEA
                     CNKIDSGNRVICVKGDVMQNFYLIRVENTKMNKIITKNGDILTDKKGSLHGLGIKSVK
                     NSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT          185 a           24 c           86 g          154 t
ORIGIN      
        1 tgaaaagaaa gaagtttgtg ataagaatga tatagaacta aaaataggaa taaattttag
       61 ttgttgtgaa gctgttgaat tagtagatat atgtcatata ttttcaaata ttttagacaa
      121 tgcaattgag gcatgtaata aaattgatag tggaaataga gttatttgtg ttaaaggtga
      181 tgttatgcaa aatttttatt taataagagt tgaaaatact aagatgaata agattattac
      241 taaaaatgga gatattttga cagacaaaaa aggttctctt catggacttg gaataaaaag
      301 tgtaaaaaac tctgtaaata agtatggtgg taatgttgta attgaatatg atgataaaaa
      361 atttgtagtt aaaattgcta ttccacttag aaatgtgaag aaagcagtta atcatgtgat
      421 ttaaatgtat ataaatttga tgtgtagaa
//
LOCUS       DAEPOZ010000475          432 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.484, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000475 DAEPOZ010000000
VERSION     DAEPOZ010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.484"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..183
                     /locus_tag="KR440_003784"
     CDS             <1..183
                     /locus_tag="KR440_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBH0700731.1"
                     /translation="NIDKKKTVKLEVLYRYVTGLEYISIGLFTRRLDITIIAIILVAT
                     ITVILYYLVRKRYITI"
BASE COUNT          195 a           39 c           56 g          142 t
ORIGIN      
        1 aatatagata agaaaaaaac tgttaaacta gaagtacttt atcgctatgt aacaggtctt
       61 gagtatatat caataggctt atttacaaga agattagata ttacaataat agcaataata
      121 ttagtagcta ctataacagt aatattatat tacttagtta gaaagagata tataactata
      181 taaaatctaa tagaattaat atcaaaagaa agtagcaact aatcaccagt aatgtaaaat
      241 aatttgtttt tcaaagttaa taaaagtctt ctttatggca atgatttaaa tataatcaaa
      301 tatcgtaacg gagatttttt tatgaataat ttaagaaaac aattaatcaa agaaattata
      361 taagatggag aacttataaa atctacagat ataagtagtt ttaggagtaa atacagatga
      421 cttatttcaa ag
//
LOCUS       DAEPOZ010000476          422 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.485, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000476 DAEPOZ010000000
VERSION     DAEPOZ010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.485"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            109..300
                     /locus_tag="KR440_003785"
     CDS             109..300
                     /locus_tag="KR440_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBH0700732.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
BASE COUNT          155 a           34 c           72 g          161 t
ORIGIN      
        1 ttttttgtca aaaaaaacaa aaaatacgta tttatttttt catttcggga aaactaaaca
       61 caggaattat ggactttata aaaatttaat tcttgaggag tgatataagt gaaaaaagtt
      121 gctttaattt tagctataat aggtgcgctt aactggggtg caattggtat tctaggtaca
      181 gacttaatag gtagtatctt tggtggaaca tatgaaatgg taagtagaat aatatacttc
      241 attgtaggtt tagctggatt atacttaata ccaagtttaa tgtctgatga tagagaataa
      301 tttatgcttt tatgttaaat acctcattct aaatgtttag aatgaggtat ttaattttat
      361 aaaaatatta atttgtttct tttttaatgg taaaatagat aagaattgaa gttttaaaaa
      421 ga
//
LOCUS       DAEPOZ010000477          420 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.486, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000477 DAEPOZ010000000
VERSION     DAEPOZ010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.486"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..>420
                     /locus_tag="KR440_003786"
     rRNA            <1..>420
                     /locus_tag="KR440_003786"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          106 a           97 c          130 g           87 t
ORIGIN      
        1 cgcctcccaa aaggtaacgg aggcgctcaa aggttctctc agtacggtcg gaaatcgtac
       61 gtagagtgta aaggcaaaag agagcttgat tgcaagacat acaggtcgag caaggatgaa
      121 aatcggactt agtgatccgg tggttctgcg tggaagggcc atcgctcaac ggataaaagc
      181 taccctgggg ataacaggct tatctccccc aagagtccac atcgacgggg aggtttggca
      241 cctcgatgtc ggctcatcac atcctggggc tgtagtaggt cccaagggtt gggctgttcg
      301 cccattaaag tggtacgcga gctgggttca gaacgtcgtg agacagttcg gtccctatcc
      361 gtcgcaggcg taggaaattt gagaagacct gtccttagta cgagaggacc gggatggacg
//
LOCUS       DAEPOZ010000478          380 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.487, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000478 DAEPOZ010000000
VERSION     DAEPOZ010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.487"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
BASE COUNT          152 a           26 c           36 g          166 t
ORIGIN      
        1 cttataaaat tatatataaa taataaaatt aattttacta tatacccata tatttagtta
       61 tattgcaggc agaatgtggt ttaccataag caaaaggaca ataaaaaata tttttctata
      121 tttttattat ctttttgtaa aagatttatt atttattgca ttaaattaat aggcagtaga
      181 ggcatagttg ataaaattaa tgaagaaatt aaacttatga atataaattt attattgagt
      241 tttaatatta catttattat tctctttgat tttaatattg tatatttaat aattattaca
      301 ttcgtctggt taataaataa taattaaata aatcaaatga ttttaattta ctatatgatt
      361 ataatataac gttgattttt
//
LOCUS       DAEPOZ010000479          373 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.488, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000479 DAEPOZ010000000
VERSION     DAEPOZ010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.488"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            177..293
                     /gene="rrf"
                     /locus_tag="KR440_003787"
     rRNA            177..293
                     /gene="rrf"
                     /locus_tag="KR440_003787"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            300..371
                     /locus_tag="KR440_003788"
     tRNA            300..371
                     /locus_tag="KR440_003788"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:332..334,aa:Asn,seq:gtt)
BASE COUNT          118 a           61 c           80 g          114 t
ORIGIN      
        1 tttgatgtta ttcattaaga tatatgtgca gtttttagag tgttaactct atgtaaagta
       61 ctaacttcaa ttgatgaagt atatatcgta aaaacaatta ctatttagta cttcaaaact
      121 attgacattt aaaaataaag atgttaatat aatacttgtc ttataaatag gcaatatgtg
      181 gttataatag caaagaggat acacctgttc ccattccgaa cacagaagtt aagctcttta
      241 gcgctgatgg tacttggtgg gaaactgcct gggagagtag gacgtagcca cgtgaatatt
      301 ccaaggtagc tcaatggtgg agcaaccggc tgttaaccgg taggctgtgg gttcgagccc
      361 cacccttgga gcc
//
LOCUS       DAEPOZ010000480          308 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.491, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000480 DAEPOZ010000000
VERSION     DAEPOZ010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.491"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..>308
                     /locus_tag="KR440_003789"
     CDS             <1..>308
                     /locus_tag="KR440_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBH0700733.1"
                     /translation="DVKLNLVDDKFLKCWIKSKNINKYIVDKSEYRLIYSNDIDNENT
                     NKKILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKKIMYPYKSNENRFA
                     "
BASE COUNT          150 a           18 c           55 g           85 t
ORIGIN      
        1 atgatgtaaa gttgaacctt gtagatgata aatttttaaa atgttggata aaaagtaaga
       61 atataaataa atatatagta gataaaagtg aatataggct aatatactca aatgatatag
      121 ataatgagaa tacaaataaa aagatactag atgaaattat agggctatat aaaacaaaat
      181 tagaaaacag aagagaatgt aaaagtggaa taaggaaatg gtatgaactt caatggggta
      241 gagaaaagct tttctttgaa agaaaaaaga taatgtatcc ttataaatca aatgaaaata
      301 gatttgct
//
LOCUS       DAEPOZ010000481          306 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.denovo.492, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000481 DAEPOZ010000000
VERSION     DAEPOZ010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.denovo.492"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            complement(<1..291)
                     /locus_tag="KR440_003790"
     CDS             complement(<1..291)
                     /locus_tag="KR440_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0700734.1"
                     /translation="MSADGSYTMGASIVAFSLTNGISPVVATLMAILCGCTAGLVTGI
                     LHIKFKISNLLSGILVMGMLYSINLRIMGKSNIPLFSFKHLFNGEISPIVLAL"
BASE COUNT          123 a           52 c           49 g           82 t
ORIGIN      
        1 taaggctaaa actataggag atatttcccc attgaataag tgtttaaagc taaatagagg
       61 aatatttgat tttcccatta ttcttaaatt gatagagtat agcattccca taactaaaat
      121 accagaaagt aagttagata tcttaaactt aatgtgaaga atccctgtaa caagaccagc
      181 agtacaacca cataagatag ccattaaagt tgctactaca ggagaaatac cattagtaag
      241 agaaaatgct acaatagaag cacccattgt atagctacca tcagcagaca tgtctggaaa
      301 atctag
//
LOCUS       DAEPOZ010000482         3881 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.guided.284, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000482 DAEPOZ010000000
VERSION     DAEPOZ010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.guided.284"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            19..957
                     /gene="blaCDD"
                     /locus_tag="KR440_003791"
     CDS             19..957
                     /gene="blaCDD"
                     /locus_tag="KR440_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBH0700735.1"
                     /translation="MKRKNNFIWIVILLVVVAMGVYYVGRKHNNTKQKHDKNHINSEL
                     NKSKVENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETRRSPCS
                     TFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYYKKLID
                     KVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQTMAKI
                     FEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGMLEREGD
                     TYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            1301..1984
                     /locus_tag="KR440_003792"
     CDS             1301..1984
                     /locus_tag="KR440_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBH0700736.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            1981..>3881
                     /locus_tag="KR440_003793"
     CDS             1981..>3881
                     /locus_tag="KR440_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBH0700737.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAQKSKGESFKKISETTLTIVGKVDVSTSSVIPAYTALGYLDRTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKMS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYINSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIG"
BASE COUNT         1504 a          455 c          673 g         1249 t
ORIGIN      
        1 gaggagatgg tataaagtat gaaaagaaaa aataatttta tttggatagt tattcttcta
       61 gttgttgtag ctatgggagt atattatgta ggaagaaagc ataacaacac aaaacaaaaa
      121 catgataaga atcatataaa ctctgaatta aataaatcca aagttgaaaa caatgataaa
      181 aacaaaaaag aaaaaattaa tatggttgac tatagtgact gttttgaagg tattagtgga
      241 ggagctattt tttataatac taaaaataaa gaatataata tatataacaa agaattaatt
      301 gaaacaagaa ggtcaccatg ttctactttt aaaatagttt caactttaat tggtttagaa
      361 aaaggggtaa taaactcaaa agaatcagtt atgggatatg atggtacaga gtatccaaat
      421 aaaaattgga ataaaaatct tagtttagaa gaggcattta aggagtcttg tgtttggtac
      481 tacaaaaaat tgatagataa agttgatgct aaatctgtcc aaaacatttt agatgattta
      541 aagtatggga attgcgatat tagcgaatgg gaaggagatt taaaaaatgg aaagggacat
      601 ttaaatggat tttggttaga atcttcatta caaatatctc caaaagaaca agtgcaaaca
      661 atggcaaaaa tatttgaggg agatacaaac tttaaaaaag agcatattaa tattttaaga
      721 gatattatga aaatagatgt aaatgataaa aacataaatg tatatggaaa aactggaaca
      781 ggatttgatg aaaaaaataa acgtgtggat gcatggtttg ttggtatgct agagcgtgaa
      841 ggtgacacct attattttgc tattaaatca gatgattcca ataaagaaat tacagggcca
      901 aaggtaaagg aaattgcaat taatataatt aagaaatatt atagtgtaag ggaataaact
      961 taattttatt tgtaaatatt atttacctat agtctgtaat atttattaaa taattttact
     1021 aaattgagtc tatatagagc aaaactcctt tagtacataa aatacttgct attgattatt
     1081 aaattgaata cctatagatt taaagttaat accttttaaa gactatgtaa tatattaaaa
     1141 gtataaataa aatatattgc atagtttttg ttttatatta taattttaaa taatgtgcaa
     1201 aaactaaaca atatttttaa tagtaattta tatttgtatt catgtcacta taatgtcatt
     1261 ttaggttgct aaaatttaac cataaaggag atgattgtat atggaaatat taaaatgtga
     1321 aaatctcaca aaaatatatg gttcaaatca aaccagagtg acagccctta ataatgtaaa
     1381 tttatcagtt caaaaaggtg attttgtatc cattgtagga gcatctggtt ctggcaaatc
     1441 tacattgtta catctgcttg ggggagtaga tagacctaca tctggaaaga tttatgttga
     1501 agatacagaa atatcaagtt taaaggaaga agctcttgca gtttttagac gtagaaaagt
     1561 aggtcttatt tatcaatttt ataatcttat acctaccctt gatgttagaa aaaatatatt
     1621 attacccatg ttacttgata aaagaaaggt ggatgaggat agattctctg agattgtatc
     1681 tatattggga ttatctgata gattaaacca tcttccaagt caactttcag gtggacaaca
     1741 acaaagggta tctatagcta gaagtttaat ctatagacca gctattttat tagcagatga
     1801 gccaactggt aatcttgata gaaaaaattc agaagaaatt gtagacttgt taaacttatc
     1861 aaataaacga tttaatcaga caatacttct tattactcat gatgagaaaa tagcactaga
     1921 ggcaaatcgt attgtaacta tggaagatgg agtgattgtc tctgaaaagg tggtgaagaa
     1981 atgaatattc ttcaagaata caccttagac tttgtacgac gcaataaacg tagtagtata
     2041 gctataatgt tagctatatt acttactaca accatgatgt cttctttgtg tggactgttg
     2101 tataccatgt ggacagattt tataagactt tcaattgaga aagaaggtaa ctggcatggt
     2161 gaattgtttg actatactta tggaagtgac ttaccattaa ttgaaaattt ttcttcagtt
     2221 gatgatgtaa tggtaaaagg acaatggtat gtaggcaaaa ttaatgatga gaaaagagat
     2281 tatataattt atagaaatgc tacatcagag tattggaatt ctatgccaga aaaggatact
     2341 ataatagagg gtcgtcgacc aaataatgct aatgaaattg cattatcaaa acaatatttt
     2401 gagaataatc caagtgtaaa aattggggat aaaattactc ttccaatagg agatagaatc
     2461 ctaaatggaa agcctcttga acccatagct caaaaatcga aaggggaaag ttttaaaaaa
     2521 atttcagaga ctacattgac aatagtagga aaggtagatg tttccacttc atcagtaata
     2581 cctgcatata cagctcttgg atatcttgat agaacaagta taaaacctaa tgatagtata
     2641 actgtgtact tgcgatttaa aaatataaga gacacataca aggaacttcc aaaacttgct
     2701 aaatcattgg ggtggaagac taatgaatat ggaaaataca atcttaaata taatagtaat
     2761 tatcttttaa aaatgttagt cttttcccca gaacagaaag catctatgag cagtatatca
     2821 aaattttcaa ctcctattat gtatcttaca atagcaattt ttacagtagc tgtgtttgta
     2881 atggtgattt acaacgcgtt ttcactatct gcaaatgcta gacttactca attagggata
     2941 ttatctagtg ttggtgcatc accaaaacag attaaaatgt ctgtagtgtt tgaaggtttt
     3001 ttgcttacaa ttataccatt gccaataggt ttatttttag gatggttatt gtgtaatagg
     3061 cttatagttt atattaattc agttaattat catactgatg ttccagaagt ggtatttact
     3121 tatggtattc ctgcttttct gccagcagtt ttacttacaa tagtaacagt ctggatttct
     3181 gcacttatac cagcacgaaa agtatcaaag atatctccta tagaagcaat acgacaagga
     3241 gatagtgtta aaatcaaaaa gtcgcataaa tattcattag gaaagatatt tggaatagaa
     3301 ggagaacttg catcaaacgc actacgtgct cgtaaaaaat cttatcgtac tgcaacaata
     3361 tctttaacat tatctttttt attacttaca tgttttttgc atataattgc aaatcaagaa
     3421 ggagcaaaag ctgtttatgg aagtgataat tataaggacc aaagagatat agcagtttat
     3481 ttggaaaata gtgaacctgt agagtctgct ttcattgaaa aattagataa tacagaaggt
     3541 gtaaaaagtt cactgtatta tcaaaaaatg tctgcttcca cttggctgac tgaaaaagat
     3601 gcatcaaaag aacttgcgaa aaatggagga tttaaaagta tagtttcatc taaaaaatac
     3661 tctcctatag aacgtgatgg caagtttcgt ataatttcaa atataattgg gcttgatgat
     3721 aagagtttta cagaatattg taggcaaatt tctgtagacc caaagttatt ttatgataca
     3781 aaaaatccaa cctcaattgt atataataag gaggaagata ttaatcgtag tacacgtaga
     3841 aataaagtat atatagatta tttaaatctt tcaataggag a
//
LOCUS       DAEPOZ010000483         2448 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.guided.347, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000483 DAEPOZ010000000
VERSION     DAEPOZ010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.guided.347"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            156..2294
                     /gene="vanT"
                     /locus_tag="KR440_003794"
     CDS             156..2294
                     /gene="vanT"
                     /locus_tag="KR440_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBH0700738.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
BASE COUNT          857 a          283 c          409 g          899 t
ORIGIN      
        1 attatagaaa tcttttatgt gcctattcat actaaagaag aagtgagaat ctctatacct
       61 gaacaatgtg tatatcaaat atcgggtaac aataaggatg gttttattgt gacattttgg
      121 agaaatagaa aggactaaga aatggaggaa caagaatgat taggaataaa tcttatgcag
      181 ggcttgatta ttttaggatt atagctgcat tacttataat tgcaattcat acatctccat
      241 tattatcata tagtaaaatg gctgactttg tgttaactag tataattgct cgtattgctg
      301 tgccattctt ttttatgcta tcaggctttt ttctaatttc aacatataat tataattgta
      361 ataaactgaa atcttttcta aaaaatgtat tatttattta cgggatagct attgtaattt
      421 atattccctt aaatatttac actggatatt tttccatgaa taatttagtg cccaatatta
      481 ttaaggacat tgtatttgat gggactttat atcatttatg gtatctacct gcatcaatta
      541 ttggtgcaag tattgcatac tttcttgtaa aaaaactagg tttaaagtac tcattagtaa
      601 tcactattgt tatttatgta attggtatgt ttggtgatag ttattatggt tttttagaaa
      661 aaataccact attaaagaat ttatatgggt atatttttga agtatcagat tatacacgaa
      721 atggtatatt ttttgcacct gtatttttca tacttggtgg aataatatct aataaatcta
      781 attatatgtc aactaaaaat agcttgattg gatttcttat atcattctta cttatgttat
      841 gtgagggaat gtttttacat aaactagggg tacagcgtca tgatagcatg tatattatgt
      901 tattgccatg tatgtatttc ttatttacag ccttaacatt tttaaaaggt tctcgagcta
      961 ggttattgcg aacatcatca ttaattattt atattataca tcctatgatg attgttgtta
     1021 ttcgtatgtt ttctaaaata ttcggaatac aaactgtctt ggttgacaat agccttattc
     1081 attatatagt ggtaagtata gcatctttta tagcttctat aattattata ttgattctta
     1141 aacctctaaa aaaagacttg aaaggagaac ataaggtaaa taccaataga gcttggattg
     1201 aaattgattt aaataatttg gaacataatg taaagatgat acaaaaagct atgccagaag
     1261 attgtgaatt gatggcagtt gtaaaagcta atgcctatgg tcatagtgct tttgaagtag
     1321 cgacttgtgc aaaccaaata ggtgtaaaag catttgcagt agcaacaata gatgagggga
     1381 ttgatttacg tcgatatgga atactaggag aaatattgat tcttggatat actaacccaa
     1441 taagggcaaa ggaacttcat aaatacaatc ttacacagag tgttattgac cttaatcatg
     1501 ctatctcttt aaacaaacag aaatacaaca taaaatcaca cattaaaatt gatacaggta
     1561 tgcacagact gggttttgat gttaatgatg tgaggtcaat tttaaaggca tttaaattaa
     1621 aatacttaga tatatgtggc atatttacac acttgtgtgt ttcagatagt ctgaaagatg
     1681 aagatgtaga ctttacaaat aaacaaattg aatgttttta taagttaatt gattttctgt
     1741 taaaaaaagg tataaaaatt cctaaagtac atattcaaag tagttatggt ttttttaatt
     1801 atcccaatct taagtgtaat tatgtaagaa ttggaattgc attatacggg atattaagtt
     1861 ctccaaatga tacgactaaa ttgcaacttg atttacgacc tgttttatcc ttaaaatcac
     1921 aagtaattct tattagaaaa atacaaaaag atgatagttt tggatatgga agagtcttta
     1981 ttgcaagcag agatagcgta attgctatat tatctattgg ctatgccgat ggtataccta
     2041 gaaatttatc ctgtggaaaa agttacgtaa taataaatgg ttgtagagca gcaatagtgg
     2101 gtcgaatttg catggaccaa cttgctgttg atattacaga tatacctaat gtagaagttg
     2161 gtaatacggc aataataata ggaagagata atttatctga attatctgct tctgaagttg
     2221 caaataactc atgtagtata tcgaatgaat tattgagcag agtaggaaga aggttaaatg
     2281 ttataaagaa gtaactcaat taaatagtaa tatttgttgt actgtgtaat ttattttact
     2341 ctgactagac atttttagta atattatgtc taatatatat tttttataac ttacatgaca
     2401 atttaagtta taaaaaggtg atgtttcaga tttttatttt gaaacatc
//
LOCUS       DAEPOZ010000484         2226 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.guided.364, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000484 DAEPOZ010000000
VERSION     DAEPOZ010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.guided.364"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            130..831
                     /gene="vanR"
                     /locus_tag="KR440_003795"
     CDS             130..831
                     /gene="vanR"
                     /locus_tag="KR440_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HBH0700739.1"
                     /translation="MNENILIVDDKKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KDLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            881..1963
                     /locus_tag="KR440_003796"
     CDS             881..1963
                     /locus_tag="KR440_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBH0700740.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
BASE COUNT          850 a          244 c          385 g          747 t
ORIGIN      
        1 aacacattct gatgtagtag ttcttttctt ataaaaatta gttacaaata tgcttgtaat
       61 tatttgatat gtaaatttac caatattata aatgatgacc tcgataataa ggagagtgaa
      121 aaataatcta tgaacgaaaa tattttgatt gtggatgata aaaaagaaat agcagattta
      181 attgaagtat atttaaaaaa tgaaggtttt acagtataca agttttatac aggtaaggaa
      241 gctctagaat gtattgaatc taaagatttg gatatggcta ttttagatat tatgcttcct
      301 gatattgatg gatttcatat ttgccagaaa attcgagaaa aatactatta tcctatcatt
      361 atgttaacag ctaaaattga agatatagac aagataatgg gtttaaccat aggtgctgat
      421 gattatataa caaagccatt taaccctttg gaagttgttg caagagtaaa gacacaatta
      481 agaagatata tgcgatataa taattcttat gaacaacagt cgataattgt taatgagtat
      541 gatattaaag gattgattat caataaagaa acacataaat gtagtttatt tgggaaagaa
      601 gtagcattaa caccgattga attttctatt ctttggtatt tatgtgaaca tcaaggaaaa
      661 gtggttcctt ctgaagaatt gtttgaagct gtatggggag aaaaatatct tgataataat
      721 aacacagtta tggctcatat agggcgatta cgtgaaaaaa ttcatgaacc ttctaaaaac
      781 ccgaaattta taaaaactgt atggggagta ggttatacaa ttgaaaagta aaaatattaa
      841 agtcaatcct gactttactc aagtgagaaa taaaattttt atgagaacag ctttaatggc
      901 atttatagcc attattttca tatggatttt atattctttt atattgtatg gtaagttttc
      961 tatctggata gtatcattct ttgaaaatgc ttttcgtatg gaatataaag ctgcattgaa
     1021 tttatatcag cagattttcc gtaatagcat ggagctcttc atgatactag ctatggtgat
     1081 tgtgtttttt ataatattcc gtgtttattt aaattggttt acaaaatatt ttagtgaaat
     1141 aaatcaaggt attgactctc ttatcaaaga agatgttgga gaagtggctt tatctccaga
     1201 gttattggca atagaaaaga aaataaactc aattaaacat atacttgaac aacgtaaatt
     1261 tgagacacaa atggcagaac aacgtaagaa tgaacttatt gtttatcttg cacatgattt
     1321 aaaaacacca cttacatcag ttatagggta tctgacacta cttcgagatg aaagtcagat
     1381 ttctgaagaa ctgcgaaaaa aatatttatc tatttcgttg gataaggcag agcgtcttga
     1441 agatttaatt aatgaattct ttgaaattac aaggtttaat ttatcaaata tatcacttga
     1501 atacagtaca gtaagcttaa ctcgtatgct tgaacaacta acatatgagt ttaaacctac
     1561 tttgatgagt aagaatttaa aatatacact gaatgttaca ccaaatatga tgataaggtg
     1621 tgatgttaat aaaatgcaac gtgtatttga taacttatta cgaaatgctg tttattatag
     1681 ttttgaagat actactattg aaataacagc tgtacaggag gatgattata taaagcttaa
     1741 atttataaat catagcaata cgattccaga ggaaaaactt gagcgtattt ttgaacaatt
     1801 ttacaggttg gatacatctc gtaattcaaa cagtggtaga gctgggttgg gacttgctat
     1861 agctaaagaa atagtggagc ttcataacgg gacaataaca gctcatagcg agaacaatat
     1921 aattaccttt aaggttatta ttccgattgt aggaaatttg taagaattta agtataaaaa
     1981 aataggaaat tctttaggat aaataaagag cactcttgtt attgatataa ttactgtatc
     2041 aaaacaagag tgcttttatt ttgatacagt aattatatca atagaaaaat gtgaaaatat
     2101 attatgtgga acagatgcaa ttgtaagtga aggttgattg gatttgaaat agaaaaagta
     2161 ttttaacatg tttctgtaga ttgtataaaa ataaataaat caacaaagga gaatcaaaga
     2221 tgaata
//
LOCUS       DAEPOZ010000485         1466 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.guided.401, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000485 DAEPOZ010000000
VERSION     DAEPOZ010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.guided.401"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..598
                     /locus_tag="KR440_003797"
     CDS             <1..598
                     /locus_tag="KR440_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBH0700741.1"
                     /translation="SNDYQTVYLKNVITRENIEVGDYTIYNDFYNDPRDFEKNNVLYH
                     YPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEWKLDINHITDAWD
                     NKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVSPYSIVGGVPAKIIKKR
                     FNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
     gene            complement(969..>1466)
                     /locus_tag="KR440_003798"
     CDS             complement(969..>1466)
                     /locus_tag="KR440_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBH0700742.1"
                     /translation="MIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKSGIKLSATV
                     IAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGEFTILEDKA
                     VLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYLSNQKLKRE
                     EYRRL"
BASE COUNT          530 a          204 c          171 g          561 t
ORIGIN      
        1 aagtaatgat tatcaaactg tatatcttaa aaatgtaata acaagggaaa atatagaagt
       61 tggagattac acaatatata atgactttta taatgaccca agagattttg agaaaaacaa
      121 tgtattatat cattacccta taaacaatga taaattaata attggtaagt tttgctcaat
      181 agcatgtaat gcaaaatttc taatgactag tggaaatcac tcaatgaaat caaaatccac
      241 atatactttt cccatctttt atgaagaatg gaaactcgat atcaaccata taacggacgc
      301 ttgggacaat aagggtaaca ttataattgg aaatgatgtg tggataggtt atgattctat
      361 aattatgtca ggtgtaaata ttggtgatgg tgcaattatt ggaacaagag cagtagttac
      421 taatgacgtc tcaccgtatt ctattgttgg aggagtacct gcaaaaatta taaaaaagag
      481 atttaataat gatacaattt caaaattatt agaaattaaa tggtgggatt ggccatataa
      541 aaaaattcaa tcaaacatta agcatattca atcaggaaat attgataaat tagtttaata
      601 ttttataatt tatttatatt aaactttact ttaataatcc ttttataaga gctttttcaa
      661 aatttgtata ttattttaat atatataaat ttaatacaac attaaataaa ataaaaagta
      721 aagttactta ataaaaagtt actttacttt ttattattta aattcctgtt tttattataa
      781 taggtaaaat tctattaaac tagttcacat tttcattatt attgatatat tatatatgaa
      841 tataccattt tgttttttaa taaaaaaatc atgctacatc agtagtaagc aactattaat
      901 gtagcatgat tttttgtatc taaataatct tagtatttac ctatcaatat agattgatat
      961 atcaaaaact atagtcttct atattcttct ctttttagct tttggttact tagatagtaa
     1021 tcaatttcat caattagacg ttgtttatca tcaggtaata caccttttaa attagcataa
     1081 tttgatgcat gattacttct aaatacaatt ttttcattag catctatatt ctttatcatc
     1141 tctttaattt cttgtaacac agccttatcc tctagtattg taaactctcc tgccttaagt
     1201 aaatcgtaaa gttcagtatt aggttctacc attaaactaa gtacacccac ataatctggt
     1261 gaaattgctc ctaacatttt tcctgtatca actgcatgct gatgtgtttt tttagttcct
     1321 ccaagacctg ctattacagt ggctgataat ttaatcccac tttttttaac ttttctacca
     1381 acttttatta attgttcact actaaaacct ttcttaatat cttctaatac ttcattgctt
     1441 ccactttcaa gccctagata aatcat
//
LOCUS       DAEPOZ010000486         1158 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.guided.426, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000486 DAEPOZ010000000
VERSION     DAEPOZ010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.guided.426"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            443..1141
                     /gene="vanR"
                     /locus_tag="KR440_003799"
     CDS             443..1141
                     /gene="vanR"
                     /locus_tag="KR440_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HBH0700743.1"
                     /translation="MSGKILVVDDERDIADLIEVYLTNENYIVYKFYSGLDALNCINL
                     NEFDLAILDVMLPGVNGFSLCQKIREKYTYPIIMLTAKDEETDKITGLTLGADDYVTK
                     PFRPLELVARVKAQIRRYKKYSGSLQSGEELSHIFSFLGLELNTQTYECHLNGKALSL
                     TPTEFSILHVLLKNKGSVVNSENLFHAIWKDEYYNKSNNTITVHIRHLREKLGDTVDE
                     AKYIKTIWGVGYKI"
BASE COUNT          453 a          132 c          165 g          408 t
ORIGIN      
        1 taaaaatcct cagaaaatta taacaataag aggagcagga tattatttta ataaagaata
       61 ataaatatat taatctcatt aataattgtt ataaatgtga aatgctaacg atatctaaac
      121 taaattatta taaaatagct gactgtaatt taattgataa agcctattct tctagacttt
      181 gccagttaaa ttacagtcag ttttattata tgaaaaaatg cgtaacagaa ctattatttt
      241 atagattata tttgaaaaga tagataaatt gtatatctaa atctatatat attcattttt
      301 atctttaata tatgttaaat aataaaatag ttacataata agattaagaa tttcttaata
      361 aattctaatg taaaaaatca tataaaaaaa ttaaatttag tataatatat ataaatatat
      421 atagaaagga tgtttaaagt ctatgagtgg aaaaatttta gttgtagatg acgaacgtga
      481 tatagctgat ttaatagaag tctaccttac aaatgaaaac tatattgttt ataaatttta
      541 ttctggtctt gatgcactaa actgcattaa tttaaatgaa tttgaccttg caattttaga
      601 tgttatgttg ccaggagtaa atggattttc tttatgccaa aaaatccgag aaaaatatac
      661 ttatccaatc attatgttaa cagctaagga tgaagaaaca gacaagatta caggcttaac
      721 tttaggtgca gatgattatg ttactaaacc tttccgacct ttggaacttg ttgctagagt
      781 gaaggcacag attagaagat ataagaaata cagtggaagt ttacaatctg gtgaagaact
      841 ttcacatatt ttttcttttt taggtctgga attgaatacc caaacatatg aatgccattt
      901 aaatggtaaa gctctttcac ttactccaac agagttttct attctacatg tattattgaa
      961 aaataaaggc tctgtggtaa attcagaaaa tctttttcat gctatctgga aagatgaata
     1021 ttacaataaa agtaacaata ctataacagt tcatattcgc catcttcgtg aaaaattagg
     1081 tgatactgta gatgaagcaa aatatataaa aacaatttgg ggtgttggtt acaagatttg
     1141 acacctattt ttcttgtc
//
LOCUS       DAEPOZ010000487          985 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.guided.436, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000487 DAEPOZ010000000
VERSION     DAEPOZ010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.guided.436"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..970
                     /locus_tag="KR440_003800"
     CDS             <1..970
                     /locus_tag="KR440_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBH0700744.1"
                     /translation="REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEF
                     LIPDAVSQNHIPNSAIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIG
                     CGTLSSALCMDKDKSHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRA
                     GSSFGISKIYKKEELQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEI
                     ELSKGFFDYTEKYTLKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLT
                     PSGEIIFNEVNTIPGFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
BASE COUNT          352 a          106 c          193 g          334 t
ORIGIN      
        1 aagggagggt aattggtatc attatattgg agattataat aatatccaga ataacacttg
       61 gtttgaaagg gatactgata taattccagt tattgtttcg cagaaccgtg tcaaaaatgg
      121 tattattgaa tttttaattc ctgatgctgt ttctcagaat catattccaa atagtgctat
      181 tgaatcatgt gaaaatgata ttaaatacac taaacttgat tttgtatttc ctgtattgca
      241 tggaaaaaat ggagaagatg gaacagtgca ggggttattt gaattggcag gtatacaggt
      301 tattggatgt ggtactcttt cttcagcatt atgtatggac aaagacaaat cacataaact
      361 tgttcattca gaaggtatta aagtaccaaa atcaatatta ctaaaatact cagataaatt
      421 taatgtggct gaactgtcaa aagacttaac ttatccatta tttataaaac ctgtgcgtgc
      481 aggttcatca tttggtatta gtaaaattta taaaaaagaa gagttacaag tagcagtaga
      541 cttggctttt gaacatgata atgaagtaat aattgaggaa aatattgatg gttttgaggt
      601 agggtgtgca gttttaggaa acgatgactt acttgttgga agagtagatg aaatagaatt
      661 gtcaaaagga ttttttgatt atacagaaaa atatacattg aaaagttcaa aaattcatat
      721 gccagctaga attgatttgg attgtgagaa aagaatacaa gagacagcaa aaattattta
      781 tagaactctt ggatgttctg gatttgctag agtagatatg tttttaactc catctggtga
      841 aattattttt aatgaagtca atacaattcc tggctttact tctcatagtc gttatccgaa
      901 tatgatgaaa ggtattggac tgtcttttga agaaatattg gataagataa ttgaggtgta
      961 cataaaatga tgaataatat tttat
//
LOCUS       DAEPOZ010000488          639 bp    DNA     linear   BCT 17-JUN-2021
DEFINITION  TPA_asm: Clostridioides difficile strain ZZV14-6368
            SAMN15890978-rid12466893.guided.462, whole genome shotgun sequence.
ACCESSION   DAEPOZ010000488 DAEPOZ010000000
VERSION     DAEPOZ010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15890978
            Sequence Read Archive: SRR12506241
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JUN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001067914.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001067914.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/12/2021 23:07:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,801
            CDSs (total)                      :: 3,718
            Genes (coding)                    :: 3,667
            CDSs (with protein)               :: 3,667
            Genes (RNA)                       :: 83
            rRNAs                             :: 6, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 28 of 51
            Pseudo Genes (incomplete)         :: 20 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 8 of 51
            CRISPR Arrays                     :: 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15890978-rid12466893.guided.462"
                     /strain="ZZV14-6368"
                     /isolation_source="shoe sole"
                     /db_xref="taxon:1496"
                     /country="Slovenia: Maribor"
                     /lat_lon="46.55 N 15.64 E"
                     /collection_date="2014-07-05"
                     /collected_by="NLZOH"
                     /note="genotype: 027"
     gene            <1..>639
                     /locus_tag="KR440_003801"
     CDS             <1..>639
                     /locus_tag="KR440_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBH0700745.1"
                     /translation="DKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSEDIDLIPV
                     DTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLRDNGKDFT
                     KKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYGFIERYQC
                     KKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKIE"
BASE COUNT          247 a           81 c          111 g          200 t
ORIGIN      
        1 agataaaaat aaagaagata aaatgacaaa tactatctta ttgactaaag ataaagttta
       61 tgatgggaat ctaattctag taaatgcttt tttaccagtt aaaacatctg aagatataga
      121 tttaattcct gtagatacaa ggtttccaag tattcttatg aaacgtgaag ctacaaatat
      181 tttacaaaat attttaaaaa gtatatgtgg aataaatgaa atagtacctg taagtggata
      241 tcgtactgca gaggaacaac aagatatata ttcaagctct ctgcgtgaca atggaaaaga
      301 ctttactaaa aagtttgtag cacttccaaa tcacagtgaa catcagacag ggcttgctat
      361 tgatttaggg ttgaagaaag aggttataga ttttatctgc cctgaatttc cttataatgg
      421 tatttgtaat gagttccgta aggcagcact acattatggt tttatagagc gttatcagtg
      481 taaaaaagaa gaaataacag gaatatctca agaaccatgg cattttcgtt atgtcggata
      541 tccacattct gagattatca ataaaatgaa tctcgccctt gaagaatata tagaatatat
      601 taagagattt tcgtatacaa attgtttaaa aatagaaga
//
